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Sample records for requirements specification srs

  1. Surveillance Analysis Computer System (SACS) software requirements specification (SRS)

    SciTech Connect

    Glasscock, J.A.; Flanagan, M.J.

    1995-09-01

    This document is the primary document establishing requirements for the Surveillance Analysis Computer System (SACS) Database, an Impact Level 3Q system. The purpose is to provide the customer and the performing organization with the requirements for the SACS Project.

  2. Surveillance Analysis Computer System (SACS): Software requirements specification (SRS). Revision 2

    SciTech Connect

    Glasscock, J.A.

    1995-03-08

    This document is the primary document establishing requirements for the Surveillance Analysis Computer System (SACS) database, an Impact Level 3Q system. SACS stores information on tank temperatures, surface levels, and interstitial liquid levels. This information is retrieved by the customer through a PC-based interface and is then available to a number of other software tools. The software requirements specification (SRS) describes the system requirements for the SACS Project, and follows the Standard Engineering Practices (WHC-CM-6-1), Software Practices (WHC-CM-3-10) and Quality Assurance (WHC-CM-4-2, QR 19.0) policies.

  3. Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2

    SciTech Connect

    Armstrong, Anthony A.; Mohideen, Firaz; Lima, Christopher D.

    2013-04-08

    Ubiquitin (Ub) and ubiquitin-like (Ubl) modifiers such as SUMO (also known as Smt3 in Saccharomyces cerevisiae) mediate signal transduction through post-translational modification of substrate proteins in pathways that control differentiation, apoptosis and the cell cycle, and responses to stress such as the DNA damage response. In yeast, the proliferating cell nuclear antigen PCNA (also known as Pol30) is modified by ubiquitin in response to DNA damage and by SUMO during S phase. Whereas Ub-PCNA can signal for recruitment of translesion DNA polymerases, SUMO-PCNA signals for recruitment of the anti-recombinogenic DNA helicase Srs2. It remains unclear how receptors such as Srs2 specifically recognize substrates after conjugation to Ub and Ubls. Here we show, through structural, biochemical and functional studies, that the Srs2 carboxy-terminal domain harbors tandem receptor motifs that interact independently with PCNA and SUMO and that both motifs are required to recognize SUMO-PCNA specifically. The mechanism presented is pertinent to understanding how other receptors specifically recognize Ub- and Ubl-modified substrates to facilitate signal transduction.

  4. Business System Planning Project System Requirements Specification

    SciTech Connect

    NELSON, R.E.

    2000-09-08

    The purpose of the Business Systems Planning Project System Requirements Specification (SRS) is to provide the outline and contents of the requirements for the CH2M HILL Hanford Group, Inc. (CHG) integrated business and technical information systems. The SRS will translate proposed objectives into the statement of the functions that are to be performed and data and information flows that they require. The requirements gathering methodology will use (1) facilitated group requirement sessions; (2) individual interviews; (3) surveys; and (4) document reviews. The requirements will be verified and validated through coordination of the technical requirement team and CHG Managers. The SRS document used the content and format specified in Lockheed Martin Services, Inc. Organization Standard Software Practices in conjunction with the Institute of Electrical and Electronics Engineers Standard 8340-1984 for Systems Requirements Documents.

  5. Specifics of processing SRS lidar signals in GHz frequency range

    NASA Astrophysics Data System (ADS)

    Grishkanich, A. S.; Elizarov, V. V.; Kascheev, S. V.; Zhevlakov, A. P.; Sidorov, I. S.

    2015-12-01

    One of the highly effective methods of operative remote environmental monitoring on land and water surfaces is laser sensing. It knew that the Raman scattering cross section is very small (10-25-10-27), so in some cases radiation back into captivity to the target could be a few tens of photons. For high-speed sensing, speed of processing and ease of use lidar units required for the use of appropriate hardware and software systems used for the decision of tasks of collecting, processing, storing, organizing large amounts of data.

  6. Investigating the clinical usefulness of the Social Responsiveness Scale (SRS) in a tertiary level, autism spectrum disorder specific assessment clinic.

    PubMed

    Aldridge, Fiona J; Gibbs, Vicki M; Schmidhofer, Katherine; Williams, Megan

    2012-02-01

    The Social Responsiveness Scale (SRS; Constantino and Gruber in Social Responsiveness Scale (SRS). Western Psychological Services, Los Angeles, 2005) is a commonly used screening tool for identifying children with possible autism spectrum disorder (ASD). This study investigated the relationship between SRS scores and eventual diagnostic outcome for children referred to a tertiary level, autism specific assessment service. Forty eight children (mean age = 8.10; 92% male) underwent a comprehensive ASD assessment. Parent and teacher SRS scores were subsequently compared with diagnostic outcome. Sensitivity was high (91% for parent report; 84% for teacher report), however specificity was much lower (8% for parent report; 41% for teacher report). Results demonstrate a need for caution when interpreting SRS results based on current cut-off scores, particularly in children with previously identified social developmental problems. PMID:21516433

  7. Software Requirements Specification Verifiable Fuel Cycle Simulation (VISION) Model

    SciTech Connect

    D. E. Shropshire; W. H. West

    2005-11-01

    The purpose of this Software Requirements Specification (SRS) is to define the top-level requirements for a Verifiable Fuel Cycle Simulation Model (VISION) of the Advanced Fuel Cycle (AFC). This simulation model is intended to serve a broad systems analysis and study tool applicable to work conducted as part of the AFCI (including costs estimates) and Generation IV reactor development studies.

  8. Why SRS Matters - Introduction

    SciTech Connect

    Hunt, Paul

    2015-01-21

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode provides an introduction to the SRS mission and operations.

  9. ENVIRONMENTAL SPECIFICATION REQUIREMENTS

    SciTech Connect

    TIFFT, S.R.

    2003-10-07

    Through regulations, permitting or binding negotiations, Regulators establish requirements, limits, permit conditions and Notice of Construction (NOC) conditions with which the Office of River Protection (ORP) and the Tank Farm Contractor (TFC) must comply. Operating Specifications are technical limits which are set on a process to prevent injury to personnel, or damage to the facility or environment. The main purpose of this document is to provide specification limits and recovery actions for the TFC Environmental Surveillance Program at the Hanford Site. Specification limits are given for monitoring frequencies and permissible variation of readings from an established baseline or previous reading. The requirements in this document are driven by environmental considerations and data analysis issues, rather than facility design or personnel safety issues. This document is applicable to all single-shell tank (SST) and double-shell tank (DST) waste tanks, and the associated catch tanks and receiver tanks, and transfer systems. This Tank Farm Environmental Specifications Document (ESD) implements environmental-regulatory limits on the configuration and operation of the Hanford Tank Farms facility that have been established by Regulators. This ESD contains specific field operational limits and recovery actions for compliance with airborne effluent regulations and agreements, liquid effluents regulations and agreements, and environmental tank system requirements. The scope of this ESD is limited to conditions that have direct impact on Operations Projects or that Operations/Projects have direct impact upon. This document does not supercede or replace any DOE Orders, regulatory permits, notices of construction, or Regulatory agency agreements binding on the ORP or the TFC. Refer to the appropriate regulation, permit, or NOC for an inclusive listing of requirements.

  10. Solid Waste Information and Tracking System (SWITS) Software Requirements Specification

    SciTech Connect

    MAY, D.L.

    2000-03-22

    This document is the primary document establishing requirements for the Solid Waste Information and Tracking System (SWITS) as it is converted to a client-server architecture. The purpose is to provide the customer and the performing organizations with the requirements for the SWITS in the new environment. This Software Requirement Specification (SRS) describes the system requirements for the SWITS Project, and follows the PHMC Engineering Requirements, HNF-PRO-1819, and Computer Software Qualify Assurance Requirements, HNF-PRO-309, policies. This SRS includes sections on general description, specific requirements, references, appendices, and index. The SWITS system defined in this document stores information about the solid waste inventory on the Hanford site. Waste is tracked as it is generated, analyzed, shipped, stored, and treated. In addition to inventory reports a number of reports for regulatory agencies are produced.

  11. ENVIRONMENTAL SPECIFICATION REQUIREMENTS

    SciTech Connect

    TIFFT, S.R.

    2003-11-03

    Through regulations, permitting or binding negotiations, Regulators establish requirements, limits, permit conditions and Notice of Construction (NOC) conditions with which the Office of River Protection (ORP) and the Tank Farm Contractor (TFC) must comply. Operating Specifications are technical limits which are set on a process to prevent injury to personnel, or damage to the facility or environment. The main purpose of this document is to provide specification limits and recovery actions for the TFC Environmental Surveillance Program at the Hanford Site. Specification limits are given for monitoring frequencies and permissible variation of readings from an established baseline or previous reading. The requirements in this document are driven by environmental considerations and data analysis issues, rather than facility design or personnel safety issues. This document is applicable to all SST and DST waste tanks, and the associated catch tanks and receiver tanks, and transfer systems. This Tank Farm ESD implements environmental-regulatory limits on the configuration and operation of the Hanford Tank Farms facility that have been established by Regulators. This ESD contains specific field operational limits and recovery actions for compliance with airborne effluent regulations and agreements, liquid effluents regulations and agreements, and environmental tank system requirements. The scope of this ESD is limited to conditions that have direct impact on Operations/Projects or that Operations/Projects have direct impact upon. This document does not supercede or replace any DOE Orders, regulatory permits, notices of construction, or Regulatory agency agreements binding on the ORP or the TFC. Refer to the appropriate regulation, permit, or NOC for an inclusive listing of requirements.

  12. Master Software Requirements Specification

    NASA Technical Reports Server (NTRS)

    Hu, Chaumin

    2003-01-01

    A basic function of a computational grid such as the NASA Information Power Grid (IPG) is to allow users to execute applications on remote computer systems. The Globus Resource Allocation Manager (GRAM) provides this functionality in the IPG and many other grids at this time. While the functionality provided by GRAM clients is adequate, GRAM does not support useful features such as staging several sets of files, running more than one executable in a single job submission, and maintaining historical information about execution operations. This specification is intended to provide the environmental and software functional requirements for the IPG Job Manager V2.0 being developed by AMTI for NASA.

  13. Investigating the Clinical Usefulness of the Social Responsiveness Scale (SRS) in a Tertiary Level, Autism Spectrum Disorder Specific Assessment Clinic

    ERIC Educational Resources Information Center

    Aldridge, Fiona J.; Gibbs, Vicki M.; Schmidhofer, Katherine; Williams, Megan

    2012-01-01

    The Social Responsiveness Scale (SRS; Constantino and Gruber in Social Responsiveness Scale (SRS). Western Psychological Services, Los Angeles, 2005) is a commonly used screening tool for identifying children with possible autism spectrum disorder (ASD). This study investigated the relationship between SRS scores and eventual diagnostic outcome…

  14. The influence of Savannah River discharge and changing SRS cooling water requirements on the potential entrainment of ichthyoplankton at the SRS Savannah River intakes

    SciTech Connect

    Paller, M.H.

    1992-08-01

    Entrainment (i.e., withdrawal of fish larvae and eggs in cooling water) at the SRS Savannah River intakes is greatest when periods of high river water usage coincide with low river dischargeduring the spawning season. American shad and striped bass are the two species of greatest concern because of their recreational and/or commercial importance and because they produce drifting eggs and larvae vulnerable to entrainment. In the mid-reaches of the Savannah River, American shad and striped bass spawn primarily during April and May. An analysis of Savannah River discharge during April and May 1973--1989 indicated the potential for entrainment of 4--18% of the American shad and striped bass larvae and eggs that drifted past the SRS. This analysis assumed the concurrent operation of L-, K-, and P-Reactors. Additional scenarios investigated were: (1) shutting down L- and P-Reactors, and operating K-Reactor with a recycle cooling tower; and (2) shutting down L- and P-Reactors, eliminating minimum flows to Steel Creek, and operating K-Reactor with a recycle cooling tower. The former scenario reduced potential entrainment to 0.7--3.3%, and the latter scenario reduced potential entrainment to 0.20.8%. Thus, the currently favored scenario of operating K-Reactor with a cooling tower and not operating L- and P-Reactors represents a significant lessening of the impact of SRS operations.

  15. Requirements management system browser software requirements specification

    SciTech Connect

    Frank, D.D.

    1996-10-01

    The purpose of this document is to define the essential user requirements for the Requirements Management System Browser (RMSB) application. This includes specifications for the Graphical User Interface (GUI) and the supporting database structures. The RMSB application is needed to provide an easy to use PC-based interface to browse system engineering data stored and managed in a UNIX software application. The system engineering data include functions, requirements, and architectures that make up the Tank Waste Remediation System (TWRS) technical baseline. This document also covers the requirements for a software application titled ``RMSB Data Loader (RMSB- DL)``, referred to as the ``Parser.`` The Parser is needed to read and parse a data file and load the data structure supporting the Browser.

  16. Project X functional requirements specification

    SciTech Connect

    Holmes, S.D.; Henderson, S.D.; Kephart, R.; Kerby, J.; Mishra, S.; Nagaitsev, S.; Tschirhart, R.; /Fermilab

    2011-03-01

    Project X is a multi-megawatt proton facility being developed to support intensity frontier research in elementary particle physics, with possible applications to nuclear physics and nuclear energy research, at Fermilab. A Functional Requirements Specification has been developed in order to establish performance criteria for the Project X complex in support of these multiple missions. This paper will describe the Functional Requirements for the Project X facility and the rationale for these requirements.

  17. Project X functional requirements specification

    SciTech Connect

    Holmes, S.D.; Henderson, S.D.; Kephart, R.; Kerby, J.; Kourbanis, I.; Lebedev, V.; Mishra, S.; Nagaitsev, S.; Solyak, N.; Tschirhart, R.; /Fermilab

    2012-05-01

    Project X is a multi-megawatt proton facility being developed to support a world-leading program in Intensity Frontier physics at Fermilab. The facility is designed to support programs in elementary particle and nuclear physics, with possible applications to nuclear energy research. A Functional Requirements Specification has been developed in order to establish performance criteria for the Project X complex in support of these multiple missions, and to assure that the facility is designed with sufficient upgrade capability to provide U.S. leadership for many decades to come. This paper will briefly review the previously described Functional Requirements, and then discuss their recent evolution.

  18. Flight Guidance System Requirements Specification

    NASA Technical Reports Server (NTRS)

    Miller, Steven P.; Tribble, Alan C.; Carlson, Timothy M.; Danielson, Eric J.

    2003-01-01

    This report describes a requirements specification written in the RSML-e language for the mode logic of a Flight Guidance System of a typical regional jet aircraft. This model was created as one of the first steps in a five-year project sponsored by the NASA Langley Research Center, Rockwell Collins Inc., and the Critical Systems Research Group of the University of Minnesota to develop new methods and tools to improve the safety of avionics designs. This model will be used to demonstrate the application of a variety of methods and techniques, including safety analysis of system and subsystem requirements, verification of key properties using theorem provers and model checkers, identification of potential sources mode confusion in system designs, partitioning of applications based on the criticality of system hazards, and autogeneration of avionics quality code. While this model is representative of the mode logic of a typical regional jet aircraft, it does not describe an actual or planned product. Several aspects of a full Flight Guidance System, such as recovery from failed sensors, have been omitted, and no claims are made regarding the accuracy or completeness of this specification.

  19. Srs2 and Mus81-Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates.

    PubMed

    Keyamura, Kenji; Arai, Kota; Hishida, Takashi

    2016-07-01

    Homologous recombination is an evolutionally conserved mechanism that promotes genome stability through the faithful repair of double-strand breaks and single-strand gaps in DNA, and the recovery of stalled or collapsed replication forks. Saccharomyces cerevisiae ATP-dependent DNA helicase Srs2 (a member of the highly conserved UvrD family of helicases) has multiple roles in regulating homologous recombination. A mutation (srs2K41A) resulting in a helicase-dead mutant of Srs2 was found to be lethal in diploid, but not in haploid, cells. In diploid cells, Srs2K41A caused the accumulation of inter-homolog joint molecule intermediates, increased the levels of spontaneous Rad52 foci, and induced gross chromosomal rearrangements. Srs2K41A lethality and accumulation of joint molecules were suppressed by inactivating Rad51 or deleting the Rad51-interaction domain of Srs2, whereas phosphorylation and sumoylation of Srs2 and its interaction with sumoylated proliferating cell nuclear antigen (PCNA) were not required for lethality. The structure-specific complex of crossover junction endonucleases Mus81 and Mms4 was also required for viability of diploid, but not haploid, SRS2 deletion mutants (srs2Δ), and diploid srs2Δ mus81Δ mutants accumulated joint molecule intermediates. Our data suggest that Srs2 and Mus81-Mms4 have critical roles in preventing the formation of (or in resolving) toxic inter-homolog joint molecules, which could otherwise interfere with chromosome segregation and lead to genetic instability. PMID:27390022

  20. Cdk1 targets Srs2 to complete synthesis-dependent strand annealing and to promote recombinational repair.

    PubMed

    Saponaro, Marco; Callahan, Devon; Zheng, Xiuzhong; Krejci, Lumir; Haber, James E; Klein, Hannah L; Liberi, Giordano

    2010-02-01

    Cdk1 kinase phosphorylates budding yeast Srs2, a member of UvrD protein family, displays both DNA translocation and DNA unwinding activities in vitro. Srs2 prevents homologous recombination by dismantling Rad51 filaments and is also required for double-strand break (DSB) repair. Here we examine the biological significance of Cdk1-dependent phosphorylation of Srs2, using mutants that constitutively express the phosphorylated or unphosphorylated protein isoforms. We found that Cdk1 targets Srs2 to repair DSB and, in particular, to complete synthesis-dependent strand annealing, likely controlling the disassembly of a D-loop intermediate. Cdk1-dependent phosphorylation controls turnover of Srs2 at the invading strand; and, in absence of this modification, the turnover of Rad51 is not affected. Further analysis of the recombination phenotypes of the srs2 phospho-mutants showed that Srs2 phosphorylation is not required for the removal of toxic Rad51 nucleofilaments, although it is essential for cell survival, when DNA breaks are channeled into homologous recombinational repair. Cdk1-targeted Srs2 displays a PCNA-independent role and appears to have an attenuated ability to inhibit recombination. Finally, the recombination defects of unphosphorylatable Srs2 are primarily due to unscheduled accumulation of the Srs2 protein in a sumoylated form. Thus, the Srs2 anti-recombination function in removing toxic Rad51 filaments is genetically separable from its role in promoting recombinational repair, which depends exclusively on Cdk1-dependent phosphorylation. We suggest that Cdk1 kinase counteracts unscheduled sumoylation of Srs2 and targets Srs2 to dismantle specific DNA structures, such as the D-loops, in a helicase-dependent manner during homologous recombinational repair. PMID:20195513

  1. Why SRS Matters - E Area

    SciTech Connect

    Howell, Steve; Mooneyhan, Verne; Tempel, Kevin; Bullington, Michele

    2015-03-09

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features E Area's mission and operations.

  2. Why SRS Matters - L Area

    SciTech Connect

    Hunt, Paul

    2015-01-28

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features L Area's mission and operations.

  3. Why SRS Matters - H Canyon

    SciTech Connect

    Hunt, Paul; Lewczyk, Mike; Swain, Mike

    2015-02-17

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features H Canyon's mission and operations.

  4. Why SRS Matters - F Area

    SciTech Connect

    Howell, Steve; Tadlock, Bill; Beeler, Dewitt; Gardner, Curt

    2015-02-17

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features F Area's mission and operations.

  5. Why SRS Matters - K Area

    SciTech Connect

    Hunt, Paul; Lawson, Janice

    2015-02-04

    A video series presenting an overview of the Savannah River Site (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features K Area's mission and operations.

  6. The human F-Box DNA helicase FBH1 faces Saccharomyces cerevisiae Srs2 and postreplication repair pathway roles.

    PubMed

    Chiolo, Irene; Saponaro, Marco; Baryshnikova, Anastasia; Kim, Jeong-Hoon; Seo, Yeon-Soo; Liberi, Giordano

    2007-11-01

    The Saccharomyces cerevisiae Srs2 UvrD DNA helicase controls genome integrity by preventing unscheduled recombination events. While Srs2 orthologues have been identified in prokaryotic and lower eukaryotic organisms, human orthologues of Srs2 have not been described so far. We found that the human F-box DNA helicase hFBH1 suppresses specific recombination defects of S. cerevisiae srs2 mutants, consistent with the finding that the helicase domain of hFBH1 is highly conserved with that of Srs2. Surprisingly, hFBH1 in the absence of SRS2 also suppresses the DNA damage sensitivity caused by inactivation of postreplication repair-dependent functions leading to PCNA ubiquitylation. The F-box domain of hFBH1, which is not present in Srs2, is crucial for hFBH1 functions in substituting for Srs2 and postreplication repair factors. Furthermore, our findings indicate that an intact F-box domain, acting as an SCF ubiquitin ligase, is required for the DNA damage-induced degradation of hFBH1 itself. Overall, our findings suggest that the hFBH1 helicase is a functional human orthologue of budding yeast Srs2 that also possesses self-regulation properties necessary to execute its recombination functions. PMID:17724085

  7. Srs2 and Mus81–Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates

    PubMed Central

    Keyamura, Kenji; Arai, Kota

    2016-01-01

    Homologous recombination is an evolutionally conserved mechanism that promotes genome stability through the faithful repair of double-strand breaks and single-strand gaps in DNA, and the recovery of stalled or collapsed replication forks. Saccharomyces cerevisiae ATP-dependent DNA helicase Srs2 (a member of the highly conserved UvrD family of helicases) has multiple roles in regulating homologous recombination. A mutation (srs2K41A) resulting in a helicase-dead mutant of Srs2 was found to be lethal in diploid, but not in haploid, cells. In diploid cells, Srs2K41A caused the accumulation of inter-homolog joint molecule intermediates, increased the levels of spontaneous Rad52 foci, and induced gross chromosomal rearrangements. Srs2K41A lethality and accumulation of joint molecules were suppressed by inactivating Rad51 or deleting the Rad51-interaction domain of Srs2, whereas phosphorylation and sumoylation of Srs2 and its interaction with sumoylated proliferating cell nuclear antigen (PCNA) were not required for lethality. The structure-specific complex of crossover junction endonucleases Mus81 and Mms4 was also required for viability of diploid, but not haploid, SRS2 deletion mutants (srs2Δ), and diploid srs2Δ mus81Δ mutants accumulated joint molecule intermediates. Our data suggest that Srs2 and Mus81–Mms4 have critical roles in preventing the formation of (or in resolving) toxic inter-homolog joint molecules, which could otherwise interfere with chromosome segregation and lead to genetic instability. PMID:27390022

  8. Requirements Specification Language (RSL) and supporting tools

    NASA Technical Reports Server (NTRS)

    Frincke, Deborah; Wolber, Dave; Fisher, Gene; Cohen, Gerald C.

    1992-01-01

    This document describes a general purpose Requirement Specification Language (RSL). RSL is a hybrid of features found in several popular requirement specification languages. The purpose of RSL is to describe precisely the external structure of a system comprised of hardware, software, and human processing elements. To overcome the deficiencies of informal specification languages, RSL includes facilities for mathematical specification. Two RSL interface tools are described. The Browser view contains a complete document with all details of the objects and operations. The Dataflow view is a specialized, operation-centered depiction of a specification that shows how specified operations relate in terms of inputs and outputs.

  9. Structured representation for requirements and specifications

    NASA Technical Reports Server (NTRS)

    Cohen, Gerald C.; Fisher, Gene; Frincke, Deborah; Wolber, Dave

    1991-01-01

    This document was generated in support of NASA contract NAS1-18586, Design and Validation of Digital Flight Control Systems suitable for Fly-By-Wire Applications, Task Assignment 2. Task 2 is associated with a formal representation of requirements and specifications. In particular, this document contains results associated with the development of a Wide-Spectrum Requirements Specification Language (WSRSL) that can be used to express system requirements and specifications in both stylized and formal forms. Included with this development are prototype tools to support the specification language. In addition a preliminary requirements specification methodology based on the WSRSL has been developed. Lastly, the methodology has been applied to an Advanced Subsonic Civil Transport Flight Control System.

  10. Technical requirements specification for tank waste retrieval

    SciTech Connect

    Lamberd, D.L.

    1996-09-26

    This document provides the technical requirements specification for the retrieval of waste from the underground storage tanks at the Hanford Site. All activities covered by this scope are conducted in support of the Tank Waste Remediation System (TWRS) mission.

  11. Interference suppression of SRS

    SciTech Connect

    Kochanov, V P

    2011-01-24

    The theory of three-wave SRS is developed, which takes into account nonlinear dispersion of a medium for arbitrary phases of the pump waves at the input to the medium. The effect of interference suppression of SRS is predicted for values of the total phase of the three-wave pump (2n+1){pi} (n=0, {+-}1, {+-}2...), the effect being caused by the destructive interference of polarisations of the nonresonant dipole-allowed transitions. The relation between the contributions of the linear and nonlinear dispersions to the SRS is found. It is shown that at a sufficiently large wave detuning, the anti-Stokes wave amplitude experiences spatial oscillations. (nonlinear-optics phenomena)

  12. Improvements to Technical Specifications surveillance requirements

    SciTech Connect

    Lobel, R.; Tjader, T.R.

    1992-12-01

    In August 1983 an NRC task group was formed to investigate problems with surveillance testing required by Technical Specifications, and to recommend approaches to effect improvements. NUREG-1024 ( Technical Specifications-Enhancing Safety Impact'') resulted, and it contained recommendations to review the basis for test frequencies; to ensure that the tests promote safety and do not degrade equipment; and to review surveillance tests so that they do not unnecessarily burden personnel. The Technical Specifications Improvement Program (TSIP) was established in December 1984 to provide the framework for rewriting and improving the Technical Specifications. As an element of the TSIP, all Technical Specifications surveillance requirements were comprehensively examined as recommended in NUREG-1024. The results of that effort are presented in this report. The study found that while some testing at power is essential to verify equipment and system operability, safety can be improved, equipment degradation decreased, and unnecessary personnel burden relaxed by reducing the amount of testing at power.

  13. Testing of SRS and RFETS Nylon Bag Material

    SciTech Connect

    Laurinat, J.E.

    1998-11-03

    This report compares the effects of radiation and heating on nylon bagout materials used at the Savannah River Site (SRS) and the Rocky Flats Environmental Technology Site (RFETS). Recently, to simplify the processing of sand, slag, and crucible (SS and C), FB-Line has replaced the low-density polyethylene (LDPE) and polyvinyl chloride (PVC) bags normally used to package cans of plutonium-bearing material with nylon bags. LDPE and PVC are not soluble in the nitric acid dissolver solution used in F-Canyon, so cans bagged using these materials had to be repackaged before they were added to the dissolver. Because nylon dissolves in nitric acid, cans bagged in nylon can be charged to the F-Canyon dissolvers without repackaging, thereby reducing handling requirements and personnel exposure. As part of a program to process RFETS SS and C at SRS, RFETS has also begun to use a nylon bagout material. The RFETS bag materials is made from a copolymer of nylon 6 and nylon 6.9, while the SRS material is made from a nylon 6 monomer. In addition, the SRS nylon has an anti-static agent added. The RFETS nylon is slightly softer than the SRS nylon, but does not appear to be as resistant to flex cracks initiated by contact with sharp corners of the inner can containing the SS and C.2 FB-Line Operations has asked for measurement of the effects of radiation and heating on these materials. Specifically, they have requested a comparison of the material properties of the plastics before and after irradiation, a measurement of the amount of outgassing when the plastics are heated, and a calculation of the amount of radiolytic gas generation. Testing was performed on samples taken from material that is currently used in FB-Line (color coded orange) and at RFETS. The requested tests are the same tests previously performed on the original and replacement nylon and LDPE bag materials.3,4,5. To evaluate the effect of irradiation on material properties, tensile stresses and elongations to break

  14. Utilization of SRS pond ash in controlled low strength material. Technical report

    SciTech Connect

    Langton, C.A.; Rajendran, N.

    1995-12-01

    Design mixes for Controlled Low Strength Material (CLSM) were developed which incorporate pond ashes (fly ashes) from the A-Area Ash Pile, the old F-Area Ash Basin and the D-Area Ash Basin. CLSM is a pumpable, flowable, excavatable backfill used in a variety of construction applications at SRS. Results indicate that CLSM which meets all of the SRS design specifications for backfill, can be made with the A-, D-, and F-Area pond ashes. Formulations for the design mixes are provided in this report. Use of the pond ashes may result in a cost savings for CLSM used at SRS and will utilize a by-product waste material, thereby decreasing the amount of material requiring disposal.

  15. Enterprise.SRS = Business for Success at SRS

    ScienceCinema

    None

    2012-06-14

    Goals and accomplishments of SRS. The debut of enterprise.srs, a strategic vision that will refocus site talents and efforts on developing future missions by broadening its impact in existing and new areas of national service. An expansion of people and facility in 3 areas: National Security, Clean Energy, and Environmental Stewardship.

  16. SRS ECOLOGY ENVIRONMENTAL INFORMATION DOCUMENT

    SciTech Connect

    Wike, L; Doug Martin, D; Eric Nelson, E; Nancy Halverson, N; John Mayer, J; Michael Paller, M; Rodney Riley, R; Michael Serrato, M

    2006-03-01

    The SRS Ecology Environmental Information Document (EEID) provides a source of information on the ecology of Savannah River Site (SRS). The SRS is a U.S. Department of Energy (DOE)--owned property on the upper Atlantic Coastal Plain of South Carolina, centered approximately 40 kilometers (25 miles) southeast of Augusta, Georgia. The entire site was designated a National Environmental Research Park in 1972 by the Atomic Energy Commission, the predecessor of DOE. This document summarizes and synthesizes ecological research and monitoring conducted on the three main types of ecosystems found at SRS: terrestrial, wetland and aquatic. It also summarizes the available information on the threatened and endangered species found on the Savannah River Site. SRS is located along the Savannah River and encompasses an area of 80,267 hectares (310 square miles) in three South Carolina counties. It contains diverse habitats, flora, and fauna. Habitats include upland terrestrial areas, wetlands, streams, reservoirs, and the adjacent Savannah River. These diverse habitats support a variety of plants and animals, including many commercially or recreationally valuable species and several rare, threatened, or endangered species. Soils are the basic terrestrial resource, influencing the development of terrestrial biological communities. Many different soils exist on the SRS, from hydric to well-drained, and from sand to clay. In general, SRS soils are predominantly well-drained loamy sands.

  17. General specifications covering requirements of aeronautic instruments

    NASA Technical Reports Server (NTRS)

    1917-01-01

    Report includes specifications for the use and production of instruments used in the navigation and operation of aircraft. Specifications are included for the following instruments: barometer or altimeter, compass, air speed meter, inclinometer, drift meter, tachometer, oil gauge, oil pressure gauge, gasoline gauge, gasoline flow indicator, distance indicator, barograph, angle of attack indicator, radiator temperature indicator, gasoline feed system pressure indicator, sextant, airplane director.

  18. SRS: Site ranking system for hazardous chemical and radioactive waste

    SciTech Connect

    Rechard, R.P.; Chu, M.S.Y.; Brown, S.L.

    1988-05-01

    This report describes the rationale and presents instructions for a site ranking system (SRS). SRS ranks hazardous chemical and radioactive waste sites by scoring important and readily available factors that influence risk to human health. Using SRS, sites can be ranked for purposes of detailed site investigations. SRS evaluates the relative risk as a combination of potentially exposed population, chemical toxicity, and potential exposure of release from a waste site; hence, SRS uses the same concepts found in a detailed assessment of health risk. Basing SRS on the concepts of risk assessment tends to reduce the distortion of results found in other ranking schemes. More importantly, a clear logic helps ensure the successful application of the ranking procedure and increases its versatility when modifications are necessary for unique situations. Although one can rank sites using a detailed risk assessment, it is potentially costly because of data and resources required. SRS is an efficient approach to provide an order-of-magnitude ranking, requiring only readily available data (often only descriptive) and hand calculations. Worksheets are included to make the system easier to understand and use. 88 refs., 19 figs., 58 tabs.

  19. IMCS reflight certification requirements and design specifications

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The requirements for reflight certification are established. Software requirements encompass the software programs that are resident in the PCC, DEP, PDSS, EC, or any related GSE. A design approach for the reflight software packages is recommended. These designs will be of sufficient detail to permit the implementation of reflight software. The PDSS/IMC Reflight Certification system provides the tools and mechanisms for the user to perform the reflight certification test procedures, test data capture, test data display, and test data analysis. The system as defined will be structured to permit maximum automation of reflight certification procedures and test data analysis.

  20. System requirements specification for SMART structures mode

    NASA Technical Reports Server (NTRS)

    1992-01-01

    Specified here are the functional and informational requirements for software modules which address the geometric and data modeling needs of the aerospace structural engineer. The modules are to be included as part of the Solid Modeling Aerospace Research Tool (SMART) package developed for the Vehicle Analysis Branch (VAB) at the NASA Langley Research Center (LaRC). The purpose is to precisely state what the SMART Structures modules will do, without consideration of how it will be done. Each requirement is numbered for reference in development and testing.

  1. 29 CFR 1918.52 - Specific requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Vessel's Cargo Handling Gear § 1918.52 Specific... tails, fittings, or other means of making the preventers fast on the deck shall provide strength...

  2. 29 CFR 1918.52 - Specific requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Vessel's Cargo Handling Gear § 1918.52 Specific... tails, fittings, or other means of making the preventers fast on the deck shall provide strength...

  3. 29 CFR 1918.52 - Specific requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Vessel's Cargo Handling Gear § 1918.52 Specific... tails, fittings, or other means of making the preventers fast on the deck shall provide strength...

  4. 29 CFR 1918.52 - Specific requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Vessel's Cargo Handling Gear § 1918.52 Specific... tails, fittings, or other means of making the preventers fast on the deck shall provide strength...

  5. PDSS/IMC requirements and functional specifications

    NASA Technical Reports Server (NTRS)

    1983-01-01

    The system (software and hardware) requirements for the Payload Development Support System (PDSS)/Image Motion Compensator (IMC) are provided. The PDSS/IMC system provides the capability for performing Image Motion Compensator Electronics (IMCE) flight software test, checkout, and verification and provides the capability for monitoring the IMC flight computer system during qualification testing for fault detection and fault isolation.

  6. 29 CFR 1918.52 - Specific requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requirements. (a) Preventers. (1) When preventers are used they shall be of sufficient strength for the... cast fittings, the strength of the fitting exceeds the total strength of all lines secured to it. Any tails, fittings, or other means of making the preventers fast on the deck shall provide strength...

  7. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... and air. (5) Detectors of the flame type. Methane detectors of the flame type shall be subject to the requirements of the flame-lamp schedule then in force. (c) Safety against bodily hazard. Bodily hazard with... according to the manufacturer's instructions, it shall be possible to detect at least 1 percent methane...

  8. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... and air. (5) Detectors of the flame type. Methane detectors of the flame type shall be subject to the requirements of the flame-lamp schedule then in force. (c) Safety against bodily hazard. Bodily hazard with... according to the manufacturer's instructions, it shall be possible to detect at least 1 percent methane...

  9. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... and air. (5) Detectors of the flame type. Methane detectors of the flame type shall be subject to the requirements of the flame-lamp schedule then in force. (c) Safety against bodily hazard. Bodily hazard with... according to the manufacturer's instructions, it shall be possible to detect at least 1 percent methane...

  10. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... and air. (5) Detectors of the flame type. Methane detectors of the flame type shall be subject to the requirements of the flame-lamp schedule then in force. (c) Safety against bodily hazard. Bodily hazard with... according to the manufacturer's instructions, it shall be possible to detect at least 1 percent methane...

  11. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and air. (5) Detectors of the flame type. Methane detectors of the flame type shall be subject to the requirements of the flame-lamp schedule then in force. (c) Safety against bodily hazard. Bodily hazard with... according to the manufacturer's instructions, it shall be possible to detect at least 1 percent methane...

  12. Light duty utility arm software requirements specification

    SciTech Connect

    Kiebel, G.R.

    1995-12-18

    This document defines the software requirements for the integrated control and data acquisition system of the Light Duty Utility Arm (LDUA) System. It is intended to be used to guide the design of the application software, to be a basis for assessing the application software design, and to establish what is to be tested in the finished application software product.

  13. Hitchhiker: Customer Accommodations and Requirements Specifications (CARS)

    NASA Technical Reports Server (NTRS)

    1992-01-01

    In 1984, NASA Headquarters established projects at the Goddard Space Flight Center (GSFC) and the Marshall Space Flight Center (MSFC) to develop quick-reaction carrier systems for low-cost 'flight of opportunity' or secondary payloads on the Space Transportation System (STS). One of these projects is the Hitchhiker (HH) Program. GSFC has developed a family of carrier equipment known as the Shuttle Payload of Opportunity Carrier (SPOC) system for mounting small payloads such as HH to the side of the Orbiter payload bay. The side-mounted HHs are referred to as Hitchhiker-G (HH-G). MSFC developed a cross-bay 'bridge-type' carrier structure called the Hitchhiker-M (HH-M). In 1987, responsibility for the HH-M carrier was transferred to and is now managed by the HH Project Office at the GSFC. The HH-M carrier now uses the same interchangeable SPOC avionics unit and the same electrical interfaces and services developed for HH-G. National Aeronautics and Space Administration (NASA) has created this document to acquaint potential HH system customers with the facilities NASA provides and the requirements which customers must satisfy to use these facilities. This publication defines interface items required for integrating customer equipment with the HH carrier system. Those items such as mounting equipment and electrical inputs and outputs; configuration, environmental, command, telemetry, and operational constraints are described as well as weight, power, and communications. The purpose of this publication is to help the customer understand essential integration documentation requirements and to prepare a Customer Payload Requirements (CPR) document.

  14. Rad52 Sumoylation Prevents the Toxicity of Unproductive Rad51 Filaments Independently of the Anti-Recombinase Srs2

    PubMed Central

    Dupaigne, Pauline; Maloisel, Laurent; Guerois, Raphaël; Le Cam, Eric; Veaute, Xavier; Coïc, Eric

    2013-01-01

    The budding yeast Srs2 is the archetype of helicases that regulate several aspects of homologous recombination (HR) to maintain genomic stability. Srs2 inhibits HR at replication forks and prevents high frequencies of crossing-over. Additionally, sensitivity to DNA damage and synthetic lethality with replication and recombination mutants are phenotypes that can only be attributed to another role of Srs2: the elimination of lethal intermediates formed by recombination proteins. To shed light on these intermediates, we searched for mutations that bypass the requirement of Srs2 in DNA repair without affecting HR. Remarkably, we isolated rad52-L264P, a novel allele of RAD52, a gene that encodes one of the most central recombination proteins in yeast. This mutation suppresses a broad spectrum of srs2Δ phenotypes in haploid cells, such as UV and γ-ray sensitivities as well as synthetic lethality with replication and recombination mutants, while it does not significantly affect Rad52 functions in HR and DNA repair. Extensive analysis of the genetic interactions between rad52-L264P and srs2Δ shows that rad52-L264P bypasses the requirement for Srs2 specifically for the prevention of toxic Rad51 filaments. Conversely, this Rad52 mutant cannot restore viability of srs2Δ cells that accumulate intertwined recombination intermediates which are normally processed by Srs2 post-synaptic functions. The avoidance of toxic Rad51 filaments by Rad52-L264P can be explained by a modification of its Rad51 filament mediator activity, as indicated by Chromatin immunoprecipitation and biochemical analysis. Remarkably, sensitivity to DNA damage of srs2Δ cells can also be overcome by stimulating Rad52 sumoylation through overexpression of the sumo-ligase SIZ2, or by replacing Rad52 by a Rad52-SUMO fusion protein. We propose that, like the rad52-L264P mutation, sumoylation modifies Rad52 activity thereby changing the properties of Rad51 filaments. This conclusion is strengthened by the

  15. 10 CFR 810.8 - Activities requiring specific authorization.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Activities requiring specific authorization. 810.8 Section 810.8 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.8 Activities requiring specific authorization. Unless generally authorized by § 810.7, a person requires specific authorization by the Secretary of Energy...

  16. Engineering Specifications derived from Science Requirements

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip; Arnold, William; Bevan, Ryan M.; Smith, W. Scott; Kirk, Charles S.; Postman, Marc

    2013-01-01

    Advanced Mirror Technology Development (AMTD) is a multi-year effort to systematically mature to TRL-6 the critical technologies needed to produce 4-m or larger flight-qualified UVOIR mirrors by 2018 so that a viable mission can be considered by the 2020 Decadal Review. This technology must enable missions capable of both general astrophysics & ultra-high contrast observations of exoplanets. To accomplish our objective, we use a science-driven systems engineering approach. We mature technologies required to enable the highest priority science AND result in a high-performance low-cost low-risk system.

  17. ARIES: Acquisition of Requirements and Incremental Evolution of Specifications

    NASA Technical Reports Server (NTRS)

    Roberts, Nancy A.

    1993-01-01

    This paper describes a requirements/specification environment specifically designed for large-scale software systems. This environment is called ARIES (Acquisition of Requirements and Incremental Evolution of Specifications). ARIES provides assistance to requirements analysts for developing operational specifications of systems. This development begins with the acquisition of informal system requirements. The requirements are then formalized and gradually elaborated (transformed) into formal and complete specifications. ARIES provides guidance to the user in validating formal requirements by translating them into natural language representations and graphical diagrams. ARIES also provides ways of analyzing the specification to ensure that it is correct, e.g., testing the specification against a running simulation of the system to be built. Another important ARIES feature, especially when developing large systems, is the sharing and reuse of requirements knowledge. This leads to much less duplication of effort. ARIES combines all of its features in a single environment that makes the process of capturing a formal specification quicker and easier.

  18. Roadmap to the SRS computing architecture

    SciTech Connect

    Johnson, A.

    1994-07-05

    This document outlines the major steps that must be taken by the Savannah River Site (SRS) to migrate the SRS information technology (IT) environment to the new architecture described in the Savannah River Site Computing Architecture. This document proposes an IT environment that is {open_quotes}...standards-based, data-driven, and workstation-oriented, with larger systems being utilized for the delivery of needed information to users in a client-server relationship.{close_quotes} Achieving this vision will require many substantial changes in the computing applications, systems, and supporting infrastructure at the site. This document consists of a set of roadmaps which provide explanations of the necessary changes for IT at the site and describes the milestones that must be completed to finish the migration.

  19. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Specific control system requirements. 63.20-1 Section 63.20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY BOILERS Additional Control System Requirements § 63.20-1 Specific control system requirements. In addition to the requirements found...

  20. Specific energy requirement for compacting corn stover.

    PubMed

    Mani, Sudhagar; Tabil, Lope G; Sokhansanj, Shahab

    2006-08-01

    Corn stover is a major crop residue for biomass conversion to produce chemicals and fuels. One of the problems associated with the supply of corn stover to conversion plants is the delivery of feedstock at a low cost. Corn stover has low bulk density and it is difficult to handle. In this study, chopped corn stover samples were compacted in a piston cylinder under three pressure levels (5, 10, 15 MPa) and at three moisture content levels (5%, 10%, 15% (wb)) to produce briquettes. The total energy requirement to compress and extrude briquette ranged from 12 to 30 MJ/t. The briquette density ranged from 650 to 950 kg/m3 increasing with pressure. Moisture content had also a significant effect on briquette density, durability and stability. Low moisture stover (5-10%) resulted in denser, more stable and more durable briquettes than high moisture stover (15%). PMID:16139500

  1. SRS SLUDGE BATCH QUALIFICATION AND PROCESSING; HISTORICAL PERSPECTIVE AND LESSONS LEARNED

    SciTech Connect

    Cercy, M.; Peeler, D.; Stone, M.

    2013-09-25

    This report provides a historical overview and lessons learned associated with the SRS sludge batch (SB) qualification and processing programs. The report covers the framework of the requirements for waste form acceptance, the DWPF Glass Product Control Program (GPCP), waste feed acceptance, examples of how the program complies with the specifications, an overview of the Startup Program, and a summary of continuous improvements and lessons learned. The report includes a bibliography of previous reports and briefings on the topic.

  2. MAXDOSE-SR: A routine release atmospheric dose model used at SRS

    SciTech Connect

    Simpkins, A.A.

    2000-02-09

    MAXDOSE-SR is a PC version of the dosimetry code MAXIGASP, which was used to calculate doses to the maximally exposed offsite individual for routine atmospheric releases of radioactive material at the Savannah River Site (SRS). Complete code description, verification of models, and user's manual have been included in this report. Minimal input is required to run the program, and site specific parameters are used when possible.

  3. Structuring Formal Requirements Specifications for Reuse and Product Families

    NASA Technical Reports Server (NTRS)

    Heimdahl, Mats P. E.

    2001-01-01

    In this project we have investigated how formal specifications should be structured to allow for requirements reuse, product family engineering, and ease of requirements change, The contributions of this work include (1) a requirements specification methodology specifically targeted for critical avionics applications, (2) guidelines for how to structure state-based specifications to facilitate ease of change and reuse, and (3) examples from the avionics domain demonstrating the proposed approach.

  4. 10 CFR 810.8 - Activities requiring specific authorization.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Activities requiring specific authorization. 810.8 Section 810.8 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.8 Activities requiring specific authorization. Unless generally authorized by § 810.7, a person requires...

  5. 10 CFR 810.8 - Activities requiring specific authorization.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Activities requiring specific authorization. 810.8 Section 810.8 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.8 Activities requiring specific authorization. Unless generally authorized by § 810.7, a person requires...

  6. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 4 2011-10-01 2011-10-01 false Specific EEO program requirements. 76.75 Section 76.75 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Equal Employment Opportunity Requirements § 76.75 Specific EEO program requirements. Under the terms...

  7. 49 CFR 173.62 - Specific packaging requirements for explosives.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Specific packaging requirements for explosives... SHIPPERS-GENERAL REQUIREMENTS FOR SHIPMENTS AND PACKAGINGS Definitions, Classification and Packaging for Class 1 § 173.62 Specific packaging requirements for explosives. (a) Except as provided in § 173.7...

  8. SRS ecology: Environmental information document

    SciTech Connect

    Wike, L.D.; Shipley, R.W.; Bowers, J.A.

    1993-09-01

    The purpose of this Document is to provide a source of ecological information based on the exiting knowledge gained from research conducted at the Savannah River Site. This document provides a summary and synthesis of ecological research in the three main ecosystem types found at SRS and information on the threatened and endangered species residing there.

  9. 29 CFR 1926.651 - Specific excavation requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 8 2010-07-01 2010-07-01 false Specific excavation requirements. 1926.651 Section 1926.651 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Excavations § 1926.651 Specific excavation requirements. (a)...

  10. 30 CFR 23.7 - Specific requirements for approval.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Specific requirements for approval. 23.7 Section 23.7 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR TESTING, EVALUATION, AND APPROVAL OF MINING PRODUCTS TELEPHONES AND SIGNALING DEVICES § 23.7 Specific requirements for approval. (a) The circuits external...

  11. RADIOIODINE GEOCHEMISTRY IN THE SRS SUBSURFACE ENVIRONMENT

    SciTech Connect

    Kaplan, D.; Emerson, H.; Powell, B.; Roberts, K.; Zhang, S.; Xu, C.; Schwer, K.; Li, H.; Ho, Y.; Denham, M.; Yeager, C.; Santschi, P.

    2013-05-16

    Iodine-129 is one of the key risk drivers for several Savannah River Site (SRS) performance assessments (PA), including that for the Low-Level Waste Disposal Facility in E-Area. In an effort to reduce the uncertainty associated with the conceptual model and the input values used in PA, several studies have recently been conducted dealing with radioiodine geochemistry at the SRS. The objective of this report was to review these recent studies and evaluate their implications on SRS PA calculations. For the first time, these studies measured iodine speciation in SRS groundwater and provided technical justification for assuming the presence of more strongly sorbing species (iodate and organo-iodine), and measured greater iodine sediment sorption when experiments included these newly identified species; specifically they measured greater sorption coefficients (K{sub d} values: the concentration ratio of iodine on the solid phase divided by the concentration in the aqueous phase). Based on these recent studies, new best estimates were proposed for future PA calculations. The new K{sub d} values are greater than previous recommended values. These proposed K{sub d} values reflect a better understanding of iodine geochemistry in the SRS subsurface environment, which permits reducing the associated conservatism included in the original estimates to account for uncertainty. Among the key contributing discoveries supporting the contention that the K{sub d} values should be increased are that: 1) not only iodide (I{sup -}), but also the more strongly sorbing iodate (IO{sub 3}{sup -}) species exists in SRS groundwater (average total iodine = 15% iodide, 42% iodate, and 43% organoiodine), 2) when iodine was added as iodate, the measured K{sub d} values were 2 to 6 times greater than when the iodine was added as iodide, and perhaps most importantly, 3) higher desorption (10 to 20 mL/g) than (ad)sorption (all previous studies) K{sub d} values were measured. The implications of this

  12. From Goal-Oriented Requirements to Event-B Specifications

    NASA Technical Reports Server (NTRS)

    Aziz, Benjamin; Arenas, Alvaro E.; Bicarregui, Juan; Ponsard, Christophe; Massonet, Philippe

    2009-01-01

    In goal-oriented requirements engineering methodologies, goals are structured into refinement trees from high-level system-wide goals down to fine-grained requirements assigned to specific software/ hardware/human agents that can realise them. Functional goals assigned to software agents need to be operationalised into specification of services that the agent should provide to realise those requirements. In this paper, we propose an approach for operationalising requirements into specifications expressed in the Event-B formalism. Our approach has the benefit of aiding software designers by bridging the gap between declarative requirements and operational system specifications in a rigorous manner, enabling powerful correctness proofs and allowing further refinements down to the implementation level. Our solution is based on verifying that a consistent Event-B machine exhibits properties corresponding to requirements.

  13. Electrical Bonding: A Survey of Requirement, Methods, and Specifications

    NASA Technical Reports Server (NTRS)

    Evans, R. W.

    1998-01-01

    This document provides information helpful to engineers imposing electrical bonding requirements, reviewing waiver requests, or modifying specifications on various space programs. Electrical bonding specifications and some of the processes used in the United States have been reviewed. This document discusses the specifications, the types of bonds, the intent of each, and the basic requirements where possible. Additional topics discussed are resistance versus impedance, bond straps, corrosion, finishes, and special applications.

  14. Robust requirements specifications for safety-critical systems

    NASA Astrophysics Data System (ADS)

    Saeed, A.; Anderson, T.; Delemos, R.

    1993-10-01

    Experience in safety-critical systems has shown that deviations from assumed behavior can and do cause accidents. This suggests that the development of requirements specifications for such systems should be supported with a risk analysis. In this paper, we present an approach to the development of robust requirements specifications (i.e. specifications that are adequate for the risks involved), based on qualitative and quantitative analyses.

  15. Saccharomyces cerevisiae Srs2 DNA helicase selectively blocks expansions of trinucleotide repeats.

    PubMed

    Bhattacharyya, Saumitri; Lahue, Robert S

    2004-09-01

    Trinucleotide repeats (TNRs) undergo frequent mutations in families afflicted with certain neurodegenerative disorders and in model organisms. TNR instability is modulated both by the repeat tract itself and by cellular proteins. Here we identified the Saccharomyces cerevisiae DNA helicase Srs2 as a potent and selective inhibitor of expansions. srs2 mutants had up to 40-fold increased expansion rates of CTG, CAG, and CGG repeats. The expansion phenotype was specific, as mutation rates at dinucleotide repeats, at unique sequences, or for TNR contractions in srs2 mutants were not altered. Srs2 is known to suppress inappropriate genetic recombination; however, the TNR expansion phenotype of srs2 mutants was largely independent of RAD51 and RAD52. Instead, Srs2 mainly functioned with DNA polymerase delta to block expansions. The helicase activity of Srs2 was important, because a point mutant lacking ATPase function was defective in blocking expansions. Purified Srs2 was substantially better than bacterial UvrD helicase at in vitro unwinding of a DNA substrate that mimicked a TNR hairpin. Disruption of the related helicase gene SGS1 did not lead to excess expansions, nor did wild-type SGS1 suppress the expansion phenotype of an srs2 strain. We conclude that Srs2 selectively blocks triplet repeat expansions through its helicase activity and primarily in conjunction with polymerase delta. PMID:15314145

  16. RADIOLYTIC HYDROGEN GENERATION INSAVANNAH RIVER SITE (SRS) HIGH LEVEL WASTETANKS COMPARISON OF SRS AND HANFORDMODELING PREDICTIONS

    SciTech Connect

    Crawford, C; Ned Bibler, N

    2009-04-15

    the waste. The difference in the two models has led to the questions of how different are the results predicted by the two models and which model predicts the more conservative (larger) G values. More conservative G values would predict higher H{sub 2} generation rates that would require higher ventilation rates in the SRS tanks. This report compares predictions based on the two models at various nitrate and nitrite concentrations in the SRS HLW tanks for both beta/gamma and for alpha radiation. It also compares predicted G values with those determined by actually measuring the H{sub 2} production from four SRS HLW tanks (Tanks 32H, 35H, 39H, and 42H). Lastly, the H{sub 2} generation rates predicted by the two models are compared for the 47 active SRS high level waste tanks using the most recent tank nitrate and nitrite concentrations and the beta/gamma and alpha heat loads for each tank. The predictions of the models for total H{sub 2} generation rates from the 47 active SRS waste were, for the most part, similar. For example, the predictions for both models applied to 25 tanks agreed within {+-}10% of each other. For the remaining 22 tanks, the SRS prediction was more conservative for 9 tanks (maximum 29% higher) and the Hanford prediction was more conservative for 13 tanks (maximum 19% higher). When comparing G values predicted by the equations presuming only alpha radiation or only beta/gamma was present the results were somewhat different. The results of predictions for alpha radiation, at the 47 current nitrate and nitrite concentrations in the SRS tanks indicated that all the SRS predictions were higher (up to 30%) than the Hanford predictions and thus more conservative. For beta/gamma radiation the predictions for both models agreed to {+-}10% for 18 of the combinations, the Hanford model predicted higher values (11 up to 17%) for 25 of the concentrations considered, and the SRS model predicted higher G values for the remaining two combinations (12 and 17

  17. UML activity diagrams in requirements specification of logic controllers

    NASA Astrophysics Data System (ADS)

    Grobelna, Iwona; Grobelny, Michał

    2015-12-01

    Logic controller specification can be prepared using various techniques. One of them is the wide understandable and user-friendly UML language and its activity diagrams. Using formal methods during the design phase increases the assurance that implemented system meets the project requirements. In the approach we use the model checking technique to formally verify a specification against user-defined behavioral requirements. The properties are usually defined as temporal logic formulas. In the paper we propose to use UML activity diagrams in requirements definition and then to formalize them as temporal logic formulas. As a result, UML activity diagrams can be used both for logic controller specification and for requirements definition, what simplifies the specification and verification process.

  18. 40 CFR 35.3550 - Specific capitalization grant agreement requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Drinking Water State Revolving Funds § 35.3550 Specific capitalization grant agreement requirements. (a) General. A State must agree to comply with this subpart, the general grant regulations at 40 CFR part 31, and specific conditions of the grant. A...

  19. 40 CFR 35.3135 - Specific capitalization grant agreement requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Specific capitalization grant agreement requirements. 35.3135 Section 35.3135 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE State Water Pollution Control Revolving Funds § 35.3135 Specific capitalization...

  20. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... addition to the requirements found in ASME CSD-1 (incorporated by reference; see 46 CFR 63.05-1), the... 46 Shipping 2 2010-10-01 2010-10-01 false Specific control system requirements. 63.20-1 Section 63.20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING...

  1. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Equal Employment Opportunity Requirements § 76.75 Specific EEO... necessary. Nothing in this section shall be interpreted to require a multichannel video programming...) In addition to using such recruitment sources, a multichannel video programming...

  2. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Equal Employment Opportunity Requirements § 76.75 Specific EEO... necessary. Nothing in this section shall be interpreted to require a multichannel video programming...) In addition to using such recruitment sources, a multichannel video programming...

  3. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Equal Employment Opportunity Requirements § 76.75 Specific EEO... necessary. Nothing in this section shall be interpreted to require a multichannel video programming...) In addition to using such recruitment sources, a multichannel video programming...

  4. SRS tank closure. Innovative technology summary report

    SciTech Connect

    Not Available

    1999-08-01

    High-level waste (HLW) tank closure technology is designed to stabilize any remaining radionuclides and hazardous constituents left in a tank after bulk waste removal. Two Savannah River Site (SRS) HLW tanks were closed after cleansing and then filling each tank with three layers of grout. The first layer consists of a chemically reducing grout. The fill material has chemical properties that retard the movement of some radionuclides and chemical constituents. A layer of controlled low-strength material (CLSM), a self-leveling fill material, is placed on top of the reducing grout. CLSM provides sufficient strength to support the overbearing weight. The final layer is a free-flowing, strong grout similar to normal concrete. After the main tank cavity is filled, risers are filled with grout, and all waste transfer piping connected to the tank is isolated. The tank ventilation system is dismantled, and the remaining systems are isolated. Equipment that remains with the tank is filled with grout. The tank and ancillary systems are left in a state requiring only limited surveillance. Administrative procedures are in place to control land use and access. DOE eventually plans to remove all of its HLW storage tanks from service. These tanks are located at SRS, Hanford, and Idaho National Engineering and Environmental Laboratory. Low-activity waste storage tanks at Oak Ridge Reservation are also scheduled for closure.

  5. Methodology for Estimating Ingestion Dose for Emergency Response at SRS

    SciTech Connect

    Simpkins, A.A.

    2003-07-21

    At the Savannah River Site (SRS), emergency response computer models are used to estimate dose following releases of radioactive materials to the environment. Downwind air and ground concentrations and their associated doses from inhalation and ground shine pathways are estimated. The emergency response model (PUFF-PLUME) uses real-time data to track either instantaneous (puff) or continuous (plume) releases. A site-specific ingestion dose model was developed for use with PUFF-PLUME that includes the following ingestion dose pathways pertinent to the surrounding SRS area: milk, beef, water, and fish. The model is simplistic and can be used with existing code output.

  6. Technical Review of SRS Dose Reconstrruction Methods Used By CDC

    SciTech Connect

    Simpkins, Ali, A

    2005-07-20

    At the request of the Centers for Disease Control and Prevention (CDC), a subcontractor Advanced Technologies and Laboratories International, Inc.(ATL) issued a draft report estimating offsite dose as a result of Savannah River Site operations for the period 1954-1992 in support of Phase III of the SRS Dose Reconstruction Project. The doses reported by ATL differed than those previously estimated by Savannah River Site SRS dose modelers for a variety of reasons, but primarily because (1) ATL used different source terms, (2) ATL considered trespasser/poacher scenarios and (3) ATL did not consistently use site-specific parameters or correct usage parameters. The receptors with the highest dose from atmospheric and liquid pathways were within about a factor of four greater than dose values previously reported by SRS. A complete set of technical comments have also been included.

  7. Independent verification and validation of large software requirement specification databases

    SciTech Connect

    Twitchell, K.E.

    1992-04-01

    To enhance quality, an independent verification and validation (IV V) review is conducted as software requirements are defined. Requirements are inspected for consistency and completeness. IV V strives to detect defects early in the software development life cycle and to prevent problems before they occur. The IV V review process of a massive software requirements specification, the Reserve Component Automation System (RCAS) Functional Description (FD) is explored. Analysis of the RCAS FD error history determined that there are no predictors of errors. The size of the FD mandates electronic analysis of the databases. Software which successfully performs automated consistency and completeness checks is discussed. The process of verifying the quality of analysis software is described. The use of intuitive ad hoc techniques, in addition to the automatic analysis of the databases, is required because of the varying content of the requirements databases. The ad hoc investigation process is discussed. Case studies are provided to illustrate how the process works. This thesis demonstrates that it is possible to perform an IV V review on a massive software requirements specification. Automatic analysis enables inspecting for completeness and consistency. The work with the RCAS FD clearly indicates that the IV V review process is not static; it must continually grow, adapt, and change as conditions warrant. The ad hoc investigation process provides this required flexibility This process also analyzes errors discovered by manual review and automatic processing. The analysis results in the development of new algorithms and the addition of new programs to the automatic inspection software.

  8. Information Requirements Specification II: Brainstorming Collective Decision-Making Technique.

    ERIC Educational Resources Information Center

    Telem, Moshe

    1988-01-01

    Information requirements specification (IRS) constitutes an Achilles heel in the system life cycle of management information systems. This article establishes a systematic overall IRS technique applicable to organizations of all types and sizes. The technique's integration of brainstorming and theory Z principles creates an effective, stimulating,…

  9. 18 CFR 12.35 - Specific inspection requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Specific inspection requirements. 12.35 Section 12.35 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT SAFETY OF WATER POWER PROJECTS...

  10. 18 CFR 12.35 - Specific inspection requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Specific inspection requirements. 12.35 Section 12.35 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT SAFETY OF WATER POWER PROJECTS...

  11. 30 CFR 23.7 - Specific requirements for approval.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., EVALUATION, AND APPROVAL OF MINING PRODUCTS TELEPHONES AND SIGNALING DEVICES § 23.7 Specific requirements for approval. (a) The circuits external to telephones and signal devices shall be intrinsically safe; that is... line power fails or is cut off. Manufacturers shall furnish instructions for the proper maintenance...

  12. 30 CFR 23.7 - Specific requirements for approval.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., EVALUATION, AND APPROVAL OF MINING PRODUCTS TELEPHONES AND SIGNALING DEVICES § 23.7 Specific requirements for approval. (a) The circuits external to telephones and signal devices shall be intrinsically safe; that is... line power fails or is cut off. Manufacturers shall furnish instructions for the proper maintenance...

  13. 30 CFR 23.7 - Specific requirements for approval.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., EVALUATION, AND APPROVAL OF MINING PRODUCTS TELEPHONES AND SIGNALING DEVICES § 23.7 Specific requirements for approval. (a) The circuits external to telephones and signal devices shall be intrinsically safe; that is... line power fails or is cut off. Manufacturers shall furnish instructions for the proper maintenance...

  14. 30 CFR 23.7 - Specific requirements for approval.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., EVALUATION, AND APPROVAL OF MINING PRODUCTS TELEPHONES AND SIGNALING DEVICES § 23.7 Specific requirements for approval. (a) The circuits external to telephones and signal devices shall be intrinsically safe; that is... line power fails or is cut off. Manufacturers shall furnish instructions for the proper maintenance...

  15. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... of part 107 of this chapter; or (2) For DOT Specifications 3B, 3BN, 3E, 4B, 4BA, 4D (water capacity... 3T (§ 178.45), if applicable. (3) Marking exceptions. A DOT 3E cylinder is not required to be...

  16. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... of part 107 of this chapter; or (2) For DOT Specifications 3B, 3BN, 3E, 4B, 4BA, 4D (water capacity... 3T (§ 178.45), if applicable. (3) Marking exceptions. A DOT 3E cylinder is not required to be...

  17. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of part 107 of this chapter; or (2) For DOT Specifications 3B, 3BN, 3E, 4B, 4BA, 4D (water capacity... 3T (§ 178.45), if applicable. (3) Marking exceptions. A DOT 3E cylinder is not required to be...

  18. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... of part 107 of this chapter; or (2) For DOT Specifications 3B, 3BN, 3E, 4B, 4BA, 4D (water capacity... 3T (§ 178.45), if applicable. (3) Marking exceptions. A DOT 3E cylinder is not required to be...

  19. 40 CFR 35.3550 - Specific capitalization grant agreement requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... subpart, the general grant regulations at 40 CFR part 31, and specific conditions of the grant. A State... match requirements associated with subsequent capitalization grants. (h) Provide match for State program... with all applicable Federal cross-cutting authorities. (p) Comply with provisions to avoid...

  20. 24 CFR 1003.302 - Project specific threshold requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Project specific threshold requirements. 1003.302 Section 1003.302 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR PUBLIC AND INDIAN HOUSING, DEPARTMENT OF HOUSING...

  1. 24 CFR 1003.302 - Project specific threshold requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Project specific threshold requirements. 1003.302 Section 1003.302 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN DEVELOPMENT (CONTINUED) OFFICE OF ASSISTANT SECRETARY FOR PUBLIC AND INDIAN HOUSING, DEPARTMENT OF HOUSING...

  2. 24 CFR 1003.302 - Project specific threshold requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 4 2014-04-01 2014-04-01 false Project specific threshold requirements. 1003.302 Section 1003.302 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN DEVELOPMENT (CONTINUED) OFFICE OF ASSISTANT SECRETARY FOR PUBLIC AND INDIAN HOUSING, DEPARTMENT OF HOUSING...

  3. 24 CFR 1003.302 - Project specific threshold requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 4 2013-04-01 2013-04-01 false Project specific threshold requirements. 1003.302 Section 1003.302 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN DEVELOPMENT (CONTINUED) OFFICE OF ASSISTANT SECRETARY FOR PUBLIC AND INDIAN HOUSING, DEPARTMENT OF HOUSING...

  4. Draft Geologic Disposal Requirements Basis for STAD Specification

    SciTech Connect

    Ilgen, Anastasia G.; Bryan, Charles R.; Hardin, Ernest

    2015-03-25

    This document provides the basis for requirements in the current version of Performance Specification for Standardized Transportation, Aging, and Disposal Canister Systems, (FCRD-NFST-2014-0000579) that are driven by storage and geologic disposal considerations. Performance requirements for the Standardized Transportation, Aging, and Disposal (STAD) canister are given in Section 3.1 of that report. Here, the requirements are reviewed and the rationale for each provided. Note that, while FCRD-NFST-2014-0000579 provides performance specifications for other components of the STAD storage system (e.g. storage overpack, transfer and transportation casks, and others), these have no impact on the canister performance during disposal, and are not discussed here.

  5. Software requirements: Guidance and control software development specification

    NASA Technical Reports Server (NTRS)

    Withers, B. Edward; Rich, Don C.; Lowman, Douglas S.; Buckland, R. C.

    1990-01-01

    The software requirements for an implementation of Guidance and Control Software (GCS) are specified. The purpose of the GCS is to provide guidance and engine control to a planetary landing vehicle during its terminal descent onto a planetary surface and to communicate sensory information about that vehicle and its descent to some receiving device. The specification was developed using the structured analysis for real time system specification methodology by Hatley and Pirbhai and was based on a simulation program used to study the probability of success of the 1976 Viking Lander missions to Mars. Three versions of GCS are being generated for use in software error studies.

  6. Rationale for windshield glass system specification requirements for shuttle orbiter

    NASA Technical Reports Server (NTRS)

    Hayashida, K.; King, G. L.; Tesinsiky, J.; Wittenburg, D. R.

    1972-01-01

    A preliminary procurement specification for the space shuttle orbiter windshield pane, and some of the design considerations and rationale leading to its development are presented. The windshield designer is given the necessary methods and procedures for assuring glass pane structural integrity by proof test. These methods and procedures are fully developed for annealed and thermally tempered aluminosilicate, borosilicate, and soda lime glass and for annealed fused silica. Application of the method to chemically tempered glass is considered. Other considerations are vision requirements, protection against bird impact, hail, frost, rain, and meteoroids. The functional requirements of the windshield system during landing, ferrying, boost, space flight, and entry are included.

  7. Commercial Submersible Mixing Pump For SRS Tank Waste Removal - 15223

    SciTech Connect

    Hubbard, Mike; Herbert, James E.; Scheele, Patrick W.

    2015-01-12

    The Savannah River Site Tank Farms have 45 active underground waste tanks used to store and process nuclear waste materials. There are 4 different tank types, ranging in capacity from 2839 m3 to 4921 m3 (750,000 to 1,300,000 gallons). Eighteen of the tanks are older style and do not meet all current federal standards for secondary containment. The older style tanks are the initial focus of waste removal efforts for tank closure and are referred to as closure tanks. Of the original 51 underground waste tanks, six of the original 24 older style tanks have completed waste removal and are filled with grout. The insoluble waste fraction that resides within most waste tanks at SRS requires vigorous agitation to suspend the solids within the waste liquid in order to transfer this material for eventual processing into glass filled canisters at the Defense Waste Processing Facility (DWPF). SRS suspends the solid waste by use of recirculating mixing pumps. Older style tanks generally have limited riser openings which will not support larger mixing pumps, since the riser access is typically 58.4 cm (23 inches) in diameter. Agitation for these tanks has been provided by four long shafted standard slurry pumps (SLP) powered by an above tank 112KW (150 HP) electric motor. The pump shaft is lubricated and cooled in a pressurized water column that is sealed from the surrounding waste in the tank. Closure of four waste tanks has been accomplished utilizing long shafted pump technology combined with heel removal using multiple technologies. Newer style waste tanks at SRS have larger riser openings, allowing the processing of waste solids to be accomplished with four large diameter SLPs equipped with 224KW (300 HP) motors. These tanks are used to process the waste from closure tanks for DWPF. In addition to the SLPs, a 224KW (300 HP) submersible mixer pump (SMP) has also been developed and deployed within older style tanks. The SMPs are product cooled and

  8. Phase matching of four-wave interactions of SRS components in birefringent SRS-active crystals

    SciTech Connect

    Smetanin, Sergei N; Basiev, Tasoltan T

    2012-03-31

    A new method has been proposed for achieving wave vector matching in four-wave interactions of frequency components upon SRS in birefringent SRS-active crystals. The method ensures anti-Stokes wave generation and enables a substantial reduction in higher order Stokes SRS generation thresholds. Phase matching directions in BaWO{sub 4} SRS-active negative uniaxial crystals and SrWO{sub 4} SRS-active positive uniaxial crystals have been found in the wavelength range 0.4 - 0.7 {mu}m.

  9. Independent verification and validation of large software requirement specification databases

    SciTech Connect

    Twitchell, K.E.

    1992-04-01

    To enhance quality, an independent verification and validation (IV&V) review is conducted as software requirements are defined. Requirements are inspected for consistency and completeness. IV&V strives to detect defects early in the software development life cycle and to prevent problems before they occur. The IV&V review process of a massive software requirements specification, the Reserve Component Automation System (RCAS) Functional Description (FD) is explored. Analysis of the RCAS FD error history determined that there are no predictors of errors. The size of the FD mandates electronic analysis of the databases. Software which successfully performs automated consistency and completeness checks is discussed. The process of verifying the quality of analysis software is described. The use of intuitive ad hoc techniques, in addition to the automatic analysis of the databases, is required because of the varying content of the requirements databases. The ad hoc investigation process is discussed. Case studies are provided to illustrate how the process works. This thesis demonstrates that it is possible to perform an IV&V review on a massive software requirements specification. Automatic analysis enables inspecting for completeness and consistency. The work with the RCAS FD clearly indicates that the IV&V review process is not static; it must continually grow, adapt, and change as conditions warrant. The ad hoc investigation process provides this required flexibility This process also analyzes errors discovered by manual review and automatic processing. The analysis results in the development of new algorithms and the addition of new programs to the automatic inspection software.

  10. Analyzing Tabular and State-Transition Requirements Specifications in PVS

    NASA Technical Reports Server (NTRS)

    Owre, Sam; Rushby, John; Shankar, Natarajan

    1997-01-01

    We describe PVS's capabilities for representing tabular specifications of the kind advocated by Parnas and others, and show how PVS's Type Correctness Conditions (TCCs) are used to ensure certain well-formedness properties. We then show how these and other capabilities of PVS can be used to represent the AND/OR tables of Leveson and the Decision Tables of Sherry, and we demonstrate how PVS's TCCs can expose and help isolate errors in the latter. We extend this approach to represent the mode transition tables of the Software Cost Reduction (SCR) method in an attractive manner. We show how PVS can check these tables for well-formedness, and how PVS's model checking capabilities can be used to verify invariants and reachability properties of SCR requirements specifications, and inclusion relations between the behaviors of different specifications. These examples demonstrate how several capabilities of the PVS language and verification system can be used in combination to provide customized support for specific methodologies for documenting and analyzing requirements. Because they use only the standard capabilities of PVS, users can adapt and extend these customizations to suit their own needs. Those developing dedicated tools for individual methodologies may find these constructions in PVS helpful for prototyping purposes, or as a useful adjunct to a dedicated tool when the capabilities of a full theorem prover are required. The examples also illustrate the power and utility of an integrated general-purpose system such as PVS. For example, there was no need to adapt or extend the PVS model checker to make it work with SCR specifications described using the PVS TABLE construct: the model checker is applicable to any transition relation, independently of the PVS language constructs used in its definition.

  11. Action Plan for updated Chapter 15 Accident Analysis in the SRS Production Reactor SAR

    SciTech Connect

    Hightower, N.T. III; Burnett, T.W.

    1989-11-15

    This report describes the Action Plan for the upgrade of the Chapter 15 Accident Analysis in the SRS Production Reactor SAR required for K-Restart. This Action Plan will be updated periodically to reflect task accomplishments and issue resolutions.

  12. Requirements specification for nickel cadmium battery expert system

    NASA Technical Reports Server (NTRS)

    1986-01-01

    The requirements for performance, design, test, and qualification of a computer program identified as NICBES, Nickel Cadmium Battery Expert System, is established. The specific spacecraft power system configuration selected was the Hubble Space Telescope (HST) Electrical Power System (EPS) Testbed. Power for the HST comes from a system of 13 Solar Panel Arrays (SPAs) linked to 6 Nickel Cadmium Batteries which are connected to 3 Busses. An expert system, NICBES, will be developed at Martin Marietta Aerospace to recognize a testbed anomaly, identify the malfunctioning component and recommend a course of action. Besides fault diagnosis, NICBES will be able to evaluate battery status, give advice on battery status and provide decision support for the operator. These requirements are detailed.

  13. Incubator Display Software Cost Reduction Toolset Software Requirements Specification

    NASA Technical Reports Server (NTRS)

    Moran, Susanne; Jeffords, Ralph

    2005-01-01

    The Incubator Display Software Requirements Specification was initially developed by Intrinsyx Technologies Corporation (Intrinsyx) under subcontract to Lockheed Martin, Contract Number NAS2-02090, for the National Aeronautics and Space Administration (NASA) Ames Research Center (ARC) Space Station Biological Research Project (SSBRP). The Incubator Display is a User Payload Application (UPA) used to control an Incubator subrack payload for the SSBRP. The Incubator Display functions on-orbit as part of the subrack payload laptop, on the ground as part of the Communication and Data System (CDS) ground control system, and also as part of the crew training environment.

  14. Tool-Guided Domain-Specific, Systematic Requirements Management

    NASA Astrophysics Data System (ADS)

    Gerlich, Rainer; Gerlich, Ralf

    2010-08-01

    The importance of the quality of requirements for successful execution and completion of a project from a technical and contractual point of view is being recognized more and more. Many methods are targeted to improve the support for collecting requirements while still focusing on natural language. However, the ambiguities in the semantics of natural language are the biggest obstacles towards success. The approach presented in this paper focuses on the elements of a domain while keeping the expressiveness of natural names and terms and introducing clear semantics. This brings the advantage that immediate verification of the human-provided inputs is possible, immediate contributions to validation are available and inconsistencies can be detected by a tool immediately. This leads to guidance of an engineer by a tool towards consistent, complete and correct requirements - requirements of high quality - and eases maintenance for the same reasons. As most of the complexity is handled by the tool due to its good knowledge on the domain, the approach is scalable towards large specifications. Several examples of application domains are described which illustrate the universality and feasibility of the approach across domain boundaries.

  15. Energy storage specification requirements for hybrid-electric vehicle

    SciTech Connect

    Burke, A.F.

    1993-09-01

    A study has been made of energy storage unit requirements for hybrid-electric vehicles. The drivelines for these vehicles included both primary energy storage units and/or pulse power units. The primary energy storage units were sized to provide ``primary energy`` ranges up to 60 km. The total power capability of the drivelines were such that the vehicles had 0 to 100 km/h acceleration times of 10 to 12 s. The power density requirements for primary energy storage devices to be used in hybrid vehicles are much higher than that for devices to be used in electric vehicles. The energy density and power density requirements for pulse-power devices for hybrid vehicles, are not much different than those in an electric vehicle. The cycle life requirements for primary energy-storage units for hybrid vehicles are about double that for electric vehicles, because of the reduced size of the storage units in the hybrid vehicles. The cycle life for pulse-power devices for hybrid vehicles is about the same as for electric vehicles having battery load leveling. Because of the need for additional components in the hybrid driveline, the cost of the energy storage units in hybrid vehicles should be much less (at least a factor of two) than those in electric vehicles. There are no presently available energy storage units that meet all the specifications for hybrid vehicle applications, but ultracapacitors and bipolar lead-acid batteries are under development that have the potential for meeting them. If flywheel systems having a mechanical system energy density of 40 to 50 W{center_dot}h/kg and an electrical system power density of 2 to 3 kw/kg can be developed, they would have the potential of meeting specifications for primary storage and pulse power units.

  16. Measuring layer-specific depth-of-focus requirements

    NASA Astrophysics Data System (ADS)

    Liegl, Bernhard; Gabor, Allen; Brodsky, Colin; Cotte, John; Krishnan, Mahadevaiyer

    2008-03-01

    As the Rayleigh equations already tell us, improvements in imaging resolution often come at the price of a depth-offocus loss. Often we balance the resolution versus DoF dilemma without regard of the imaging layers location in the overall film stack. E.g. often several via or metal layers are processed with the same optical settings despite facing different amount of depth-of-focus requirements. In actuality, however, substrate induced focus variation can vary greatly from layers at the bottom of a film stack to the layers higher up in the film stack. In the age of super-low k1 lithography this variance needs to be taken into account on a layer specific basis when evaluating the resolution versus DoF tradeoff. We have studied substrate induced focus variation for a 45nm technology test-site as function of film stack sequence and spatial frequency, combining various measurement techniques into an overall topography spectrum. These techniques include data extraction from the exposure tools optical leveling sensor, a mechanical air gauge to calibrate the former and interferometric profiling tools. As a result, we can quantify our DoF requirement for a given layer and product and use this information to optimize our process design on a layer-by-layer basis. This work was performed by the Research Alliance Teams at various IBM Research and Development Facilities

  17. CRISPR Immunological Memory Requires a Host Factor for Specificity.

    PubMed

    Nuñez, James K; Bai, Lawrence; Harrington, Lucas B; Hinder, Tracey L; Doudna, Jennifer A

    2016-06-16

    Bacteria and archaea employ adaptive immunity against foreign genetic elements using CRISPR-Cas systems. To generate immunological memory, the Cas1-Cas2 protein complex captures 30-40 base pair segments of foreign DNA and catalyzes their integration into the host genome as unique spacer sequences. Although spacers are inserted strictly at the A-T-rich leader end of CRISPR loci in vivo, the molecular mechanism of leader-specific spacer integration remains poorly understood. Here we show that the E. coli integration host factor (IHF) protein is required for spacer acquisition in vivo and for integration into linear DNA in vitro. IHF binds to the leader sequence and induces a sharp DNA bend, allowing the Cas1-Cas2 integrase to catalyze the first integration reaction at the leader-repeat border. Together, these results reveal that Cas1-Cas2-mediated spacer integration requires IHF-induced target DNA bending and explain the elusive role of CRISPR leader sequences during spacer acquisition. PMID:27211867

  18. Continuous-Wave Stimulated Raman Scattering (cwSRS) Microscopy

    PubMed Central

    Meng, Zhaokai; Petrov, Georgi I.; Yakovlev, Vladislav V.

    2013-01-01

    Stimulated Raman scattering (SRS) microscopy is a powerful tool for chemically-sensitive non-invasive optical imaging. However, ultrafast laser sources, which are currently employed, are still expensive and require substantial maintenance to provide temporal overlap and spectral tuning. SRS imaging, which utilizes continuous-wave laser sources, has a major advantage, as it eliminates the cell damage due to exposure to the high-intensity light radiation, while substantially reducing the cost and complexity of the set-up. As a proof-of-principle, we demonstrate microscopic imaging of dimethyl sulfoxide using two independent, commonly used lasers, a diode-pumped, intracavity doubled 532-nm laser and a He-Ne laser operating at 632.8-nm PMID:24058269

  19. ANALYSIS OF SPECIAL WASTE CONFIGURATIONS AT THE SRS WASTE MANAGEMENT FACILITIES

    SciTech Connect

    Casella, V; Raymond Dewberry, R

    2007-05-14

    Job Control Waste (JCW) at the Savannah River Site (SRS) Solid Waste Management Facilities (SWMF) may be disposed of in special containers, and the analysis of these containers requires developing specific analysis methodologies. A method has been developed for the routine assay of prohibited items (liquids, etc.) contained in a 30-gallon drum that is then placed into a 55-gallon drum. Method development consisted of system calibration with a NIST standard at various drum-to-detector distances, method verification with a liquid sample containing a known amount of Pu-238, and modeling the inner container using Ortec Isotopic software. Using this method for measurement of the known standard in the drum-in-drum configuration produced excellent agreement (within 15%) with the known value. Savannah River Site Solid Waste Management also requested analysis of waste contained in large black boxes (commonly 18-feet x 12-feet x 7-feet) stored at the SWMF. These boxes are frequently stored in high background areas and background radiation must be considered for each analysis. A detection limit of less than 150 fissile-gram-equivalents (FGE) of TRU waste is required for the black-box analyses. There is usually excellent agreement for the measurements at different distances and measurement uncertainties of about 50% are obtained at distances of at least twenty feet from the box. This paper discusses the experimental setup, analysis and data evaluation for drum-in-drum and black box waste configurations at SRS.

  20. Savannah River Site (SRS) environmental overview

    SciTech Connect

    O'Rear, M.G. ); Steele, J.L.; Kitchen, B.G. )

    1990-01-01

    The environmental surveillance activities at and in the vicinity of the Savannah River Site (SRS) (formerly the Savannah River Plant (SRP)) comprise one of the most comprehensive and extensive environmental monitoring programs in the United States. This overview contains monitoring data from routine and nonroutine radiological and nonradiological environmental surveillance activities, summaries of environmental protection programs in progress, a summary of National Environmental Policy Act (NEPA) activities, and a listing of environmental permits (Appendix A) issued by regulatory agencies. This overview provides information about the impact of SRS operations on the public and the environment. The SRS occupies a large area of approximately 300 square miles along the Savannah River, principally in Aiken and Barnwell counties of South Carolina. SRS's primary function is the production of tritium, plutonium, and other special nuclear materials for national defense, for other governmental uses, and for some civilian purposes. From August 1950 to March 31, 1989, SRS was operated for the Department of Energy (DOE) by E. I. du Pont de Nemours Co. On April 1, 1989 the Westinghouse Savannah River Company assumed responsibility as the prime contractor for the Savannah River Site.

  1. SRS Computer Animation and Drive Train System

    NASA Technical Reports Server (NTRS)

    Arthun, Daniel; Schachner, Christian

    2001-01-01

    The spinning rocket simulator (SRS) is an ongoing project at Oral Roberts University. The goal of the SRS is to gather crucial data concerning a spinning rocket under thrust for the purpose of analysis and correction of the coning motion experienced by this type of spacecraft maneuver. The computer animation simulates a virtual, scale model of the component of the SRS that represents the spacecraft itself. This component is known as the (VSM), or virtual spacecraft model. During actual physical simulation, this component of the SRS will experience a coning. The goal of the animation is to cone the VSM within that range to accurately represent the motion of the actual simulator. The drive system of the SRS is the apparatus that turns the actual simulator. It consists of a drive motor, motor mount and chain to power the simulator into motion. The motor mount is adjustable and rigid for high torque application. A digital stepper motor controller actuates the main drive motor for linear acceleration. The chain transfers power from the motor to the simulator via sprockets on both ends.

  2. Seismic Hazard Characterization at the DOE Savannah River Site (SRS): Status report

    SciTech Connect

    Savy, J.B.

    1994-06-24

    The purpose of the Seismic Hazard Characterization project for the Savannah River Site (SRS-SHC) is to develop estimates of the seismic hazard for several locations within the SRS. Given the differences in the geology and geotechnical characteristics at each location, the estimates of the seismic hazard are to allow for the specific local conditions at each site. Characterization of seismic hazard is a critical factor for the design of new facilities as well as for the review and potential retrofit of existing facilities at SRS. The scope of the SRS seismic hazard characterization reported in this document is limited to the Probabilistic Seismic Hazard Analysis (PSHA). The goal of the project is to provide seismic hazard estimates based on a state-of-the-art method which is consistent with developments and findings of several ongoing studies which are deemed to bring improvements in the state of the seismic hazard analyses.

  3. Examining Reuse in LaSRS++-Based Projects

    NASA Technical Reports Server (NTRS)

    Madden, Michael M.

    2001-01-01

    NASA Langley Research Center (LaRC) developed the Langley Standard Real-Time Simulation in C++ (LaSRS++) to consolidate all software development for its simulation facilities under one common framework. A common framework promised a decrease in the total development effort for a new simulation by encouraging software reuse. To judge the success of LaSRS++ in this regard, reuse metrics were extracted from 11 aircraft models. Three methods that employ static analysis of the code were used to identify the reusable components. For the method that provides the best estimate, reuse levels fall between 66% and 95% indicating a high degree of reuse. Additional metrics provide insight into the extent of the foundation that LaSRS++ provides to new simulation projects. When creating variants of an aircraft, LaRC developers use object-oriented design to manage the aircraft as a reusable resource. Variants modify the aircraft for a research project or embody an alternate configuration of the aircraft. The variants inherit from the aircraft model. The variants use polymorphism to extend or redefine aircraft behaviors to meet the research requirements or to match the alternate configuration. Reuse level metrics were extracted from 10 variants. Reuse levels of aircraft by variants were 60% - 99%.

  4. [A study for time-history waveform synthesis of algorithm in shock response spectrum (SRS)].

    PubMed

    Liu, Hong-ying; Ma, Ai-jun

    2002-12-01

    Objective. To present an effective on-line SRS time-history waveform synthesis method for simulating pyrotechnic shock environment with electrodynamic shakers. Method. A procedure was developed for synthesizing a SRS time-history waveform according to a general principle. The effect of three main parameters to waveform's shape, amplitude of acceleration and duration were investigated. A modification method of SRS's amplitude and an optimal algorithm of time-history waveform were presented. Result. The algorithm was used to generate a time-history waveform that could satisfy SRS's accuracy requirement and electrodynamic shaker's acceleration limitation. Conclusion. The numerical example indicates that the developed method is effective. The synthesized time-history waveform can be used to simulate pyrotechnic shock environment using electrodynamic shakers. PMID:12622083

  5. SRS Public Involvement in Waste Management Has Resulted in Effective Decisions Supported by the Public Including Disposal Changes and Top-to-Bottom Review Initiative Consensus

    SciTech Connect

    Goldston, W. T.; Villasor, H. P.

    2003-02-27

    In the Savannah River Site's (SRS') Solid Waste Management Program, a key to success is the Public Involvement Program. The Solid Waste Division at SRS manages the site's transuranic, low-level, mixed, and hazardous wastes. All decisions associated with management of this waste are of interest to the public and successful program implementation would be impossible without a vigorous public involvement program. The SRS Solid Waste Division (SWD) and its Department of Energy (DOE) customer developed, implemented, and maintain a comprehensive public participation and communications program. It is staffed by public participation and technical specialists to ensure information is presented in a manner that is technically accurate while being tailored for understanding by people without a technical background. The program provides the public with accurate, complete, timely information and early meaningful participation opportunities. It also fulfills the public participation activities required by laws, regulations, DOE Orders, and negotiated agreements. The primary goal of the SWD Public Participation Program is to fulfill the objectives of the SWD and SRS Strategic Plans to ''build trust and communicate openly, honestly, and responsibly with employees, customers, stakeholders, and regulators,'' and to ''work to extend the support of external stakeholders for the pursuit of SRS and DOE Complex business goals.'' This paper focuses on the public participation program goals, the implementation through formal plans and objectives, targeted waste management programs and specific audiences, and specific effects of the program on waste management activities. A discussion of the DOE and contractor teaming along with how plans are carried out is also included.

  6. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of part 107 of this chapter; or (2) For DOT Specifications 3B, 3BN, 3E, 4B, 4BA, 4D (water capacity... specification 3HT (§ 178.44) or 3T (§ 178.45), if applicable. (3) Marking exceptions. A DOT 3E cylinder is...

  7. Mixing in SRS Closure Business Unit Applications

    SciTech Connect

    POIRIER, MICHAELR.

    2004-06-23

    The following equipment is commonly used to mix fluids: mechanical agitators, jets (pumps), shrouded axial impeller mixers (Flygt mixers), spargers, pulsed jet mixers, boiling, static mixers, falling films, liquid sprays, and thermal convection. This discussion will focus on mechanical agitators, jets, shrouded axial impeller mixers, spargers, and pulsed jet mixers, as these devices are most likely to be employed in Savannah River Site (SRS) Closure Business applications. In addressing mixing problems in the SRS Tank Farm, one must distinguish between different mixing objectives. These objectives include sludge mixing (e.g., Extended Sludge Processing), sludge retrieval (e.g., sludge transfers between tanks), heel retrieval (e.g., Tanks 18F and 19F), chemical reactions (e.g., oxalic acid neutralization) and salt dissolution. For example, one should not apply sludge mixing guidelines to heel removal applications. Mixing effectiveness is a function of both the mixing device (e.g., slurry pump, agitator, air sparger) and the properties of the material to be mixed (e.g., yield stress, viscosity, density, and particle size). The objective of this document is to provide background mixing knowledge for the SRS Closure Business Unit personnel and to provide general recommendations for mixing in SRS applications.

  8. SRS Geology/Hydrogeology Environmental Information Document

    SciTech Connect

    Denham, M.E.

    1999-08-31

    The purpose of the Savannah River Site Geology and Hydrogeology Environmental Information Document (EID) is to provide geologic and hydrogeologic information to serve as a baseline to evaluate potential environmental impacts. This EID is based on a summary of knowledge accumulated from research conducted at the Savannah River Site (SRS) and surrounding areas.

  9. Groundwater Treatment at SRS: An Innovative Approach

    SciTech Connect

    Jorque, M.A.; Golshir, G.H.; Davis, B.

    1998-03-01

    The SRS is located in southwestern South Carolina, occupying an almost circular area of approximately 800 km{sub 2} within Aiken, Barnwell, and Allendale counties. The site lies approximately 36 km southeast of Augusta, Georgia, and is bounded by the Savannah River along its southwestern border. Prior to the establishment of the SRS in 1952, the area was largely a rural agricultural community. As part of the defense complex, the SRS produced special nuclear materials for the national defense. From 1955 until 1988, unlined earthen basins were used to dispose of wastewater from the SRS separations facilities located in the F and H areas. Approximately 300 million liters of wastewater was transported annually from the process area through underground piping to the basins. The wastewater was allowed to evaporate and to seep into the underlying formations. There were three basins in the F-Area covering a total of about 3 hectares; while the H-Area was served by four basins covering about 6 hectares. The seepage basins closure was started in 1989 and SCDHEC certified the closures as completed in 1991. Groundwater monitoring conducted in accordance with the provisions of the RCRA Permits determined that the underlying hydrogeologic units were contaminated by tritium, radioactive metals (primarily Cesium{sup 137}, Strontium{sup 90}, and Uranium{sup 235}), nitrate and heavy metals, some of which are defined as hazardous by RCRA. Under the terms and conditions of the RCRA Post-Closure Permits, it was necessary to remediate the contaminated groundwater plumes.

  10. Groundwater Treatment at SRS: An Innovative Approach

    SciTech Connect

    Jorque, M.A.; Golshir, G.H.; Davis, B.

    1998-03-01

    The SRS is located in southwestern South Carolina, occupying an almost circular area of approximately 800 km2 within Aiken, Barnwell, and Allendale counties. The site lies approximately 36 km southeast of Augusta, Georgia, and is bounded by the Savannah River along its southwestern border. Prior to the establishment of the SRS in 1952, the area was largely a rural agricultural community. As part of the defense complex, the SRS produced special nuclear materials for the national defense.From 1955 until 1988, unlined earthen basins were used to dispose of wastewater from the SRS separations facilities located in the F and H areas. Approximately 300 million liters of wastewater was transported annually from the process area through underground piping to the basins. The wastewater was allowed to evaporate and to seep into the underlying formations. There were three basins in the F-Area covering a total of about 3 hectares; while the H-Area was served by four basins covering about 6 hectares. The seepage basins closure was started in 1989 and SCDHEC certified the closures as completed in 1991.Groundwater monitoring conducted in accordance with the provisions of the RCRA Permits determined that the underlying hydrogeologic units were contaminated by tritium, radioactive metals (primarily Cesium 137, Strontium 90, and Uranium 235), nitrate and heavy metals, some of which are defined as hazardous by RCRA. Under the terms and conditions of the RCRA Post- Closure Permits, it was necessary to remediate the contaminated groundwater plumes.

  11. SRS Tank Structural Integrity Program

    SciTech Connect

    Maryak, Matthew

    2010-11-01

    The mission of the Structural Integrity Program is to ensure continued safe management and operation of the waste tanks for whatever period of time these tanks are required. Matthew Maryak provides an overview of the Structural Integrity Program to open Session 5 (Waste Storage and Tank Inspection) of the 2010 EM Waste Processing Technical Exchange.

  12. Brief Report: the Social Responsiveness Scale for Adults (SRS-A): initial results in a German cohort.

    PubMed

    Bölte, Sven

    2012-09-01

    The Social Responsiveness Scale (SRS) is a tool for quantitative autism assessment in children and adolescents. The SRS-A addresses social responsiveness in adulthood. Reliability and validity using the German adaptation of the SRS-A was examined in 20 adults with Autism Spectrum Disorder (ASD), 62 with other mental disorders (CLIN) and 163 typically developing (TD) participants. Cronbach's alpha ranged from .71 (TD) to .89 (ASD). A SRS-A total score of 67 had a sensitivity of .85, and a specificity of .83 for ASD versus CLIN/TD. Correlations with established autism scales (ADOS, AQ, SCQ) were moderate to high (r = .25-.83). Results provide adequate preliminary support for the application of the SRS-A. PMID:22183423

  13. TRANSIENT HEAT TRANSFER MODEL FOR SRS WASTE TANK OPERATIONS

    SciTech Connect

    Lee, S; Richard Dimenna, R

    2007-03-27

    A transient heat balance model was developed to assess the impact of a Submersible Mixer Pump (SMP) on waste temperature during the process of waste mixing and removal for the Type-I Savannah River Site (SRS) tanks. The model results will be mainly used to determine the SMP design impacts on the waste tank temperature during operations and to develop a specification for a new SMP design to replace existing long-shaft mixer pumps used during waste removal. The model will also be used to provide input to the operation planning. This planning will be used as input to pump run duration in order to maintain temperature requirements within the tank during SMP operation. The analysis model took a parametric approach. A series of the modeling analyses was performed to examine how submersible mixer pumps affect tank temperature during waste removal operation in the Type-I tank. The model domain included radioactive decay heat load, two SMP's, and one Submersible Transfer Pump (STP) as heat source terms. The present model was benchmarked against the test data obtained by the tank measurement to examine the quantitative thermal response of the tank and to establish the reference conditions of the operating variables under no SMP operation. The results showed that the model predictions agreed with the test data of the waste temperatures within about 10%. Transient modeling calculations for two potential scenarios of sludge mixing and removal operations have been made to estimate transient waste temperatures within a Type-I waste tank. When two 200-HP submersible mixers and 12 active cooling coils are continuously operated in 100-in tank level and 40 C initial temperature for 40 days since the initiation of mixing operation, waste temperature rises about 9 C in 48 hours at a maximum. Sensitivity studies for the key operating variables were performed. The sensitivity results showed that the chromate cooling coil system provided the primary cooling mechanism to remove process

  14. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... entrepreneurs to conduct business with all parts of its operation. For example, this requirement may be met by: (1) Recruiting as wide as possible a pool of qualified entrepreneurs from sources such as...

  15. ENHANCED UNCERTAINTY ANALYSIS FOR SRS COMPOSITE ANALYSIS

    SciTech Connect

    Smith, F.; Phifer, M.

    2011-06-30

    sand and clay), (b) Dose Parameters (34 parameters), (c) Material Properties (20 parameters), (d) Surface Water Flows (6 parameters), and (e) Vadose and Aquifer Flow (4 parameters). Results provided an assessment of which group of parameters is most significant in the dose uncertainty. It was found that K{sub d} and the vadose/aquifer flow parameters, both of which impact transport timing, had the greatest impact on dose uncertainty. Dose parameters had an intermediate level of impact while material properties and surface water flows had little impact on dose uncertainty. Results of the importance analysis are discussed further in Section 7 of this report. The objectives of this work were to address comments received during the CA review on the uncertainty analysis and to demonstrate an improved methodology for CA uncertainty calculations as part of CA maintenance. This report partially addresses the LFRG Review Team issue of producing an enhanced CA sensitivity and uncertainty analysis. This is described in Table 1-1 which provides specific responses to pertinent CA maintenance items extracted from Section 11 of the SRS CA (2009). As noted above, the original uncertainty analysis looked at each POA separately and only included the effects from at most five sources giving the highest peak doses at each POA. Only 17 of the 152 CA sources were used in the original uncertainty analysis and the simulation time was reduced from 10,000 to 2,000 years. A major constraint on the original uncertainty analysis was the limitation of only being able to use at most four distributed processes. This work expanded the analysis to 10,000 years using 39 of the CA sources, included cumulative dose effects at downstream POAs, with more realizations (1,000) and finer time steps. This was accomplished by using the GoldSim DP-Plus module and the 36 processors available on a new windows cluster. The last part of the work looked at the contribution to overall uncertainty from the main

  16. SRS in the strong-focusing regime for Raman amplifiers.

    PubMed

    McKay, Aaron; Mildren, Richard P; Coutts, David W; Spence, David J

    2015-06-01

    The theoretical analysis of stimulated Raman scattering (SRS) in crystalline amplifiers with a tightly-focused pump geometry is presented. We predict the minimum Stokes seed power required for an efficient Raman power amplifier and verify this result experimentally. Conversion of a pump to a Stokes beam in a single-pass diamond amplifier is demonstrated using nanosecond pulses with gains of 5.8 from a 1.2-kW peak-power Stokes seed beam. The results demonstrate the possibility of amplifying and combining high-power continuous-wave lasers using current diamond Raman laser technology. PMID:26072857

  17. Treatability Variance Petition for SRS Raschig Ring Packing Material

    SciTech Connect

    Hagstrom, T.

    1999-08-31

    The Department of Energy's Savannah River Site (SRS) is a vital component in the nation's nuclear weapons complex. When in full operation, SRS produced nuclear material by manufacturing fuel and target components that were then irradiated in nuclear reactors.

  18. Groundwater Monitoring Optimization of Post Closure Waste Sites at SRS - 13184

    SciTech Connect

    Ross, Jeff; O'Quinn, Sadika; Adams, Karen; Prater, Phil

    2013-07-01

    Groundwater monitoring at the Savannah River Site (SRS) is required at dozens of waste sites and includes sampling at over 1,000 monitoring wells. The expected longevity of groundwater contamination and associated groundwater monitoring and reporting constitutes a significant long-term cost that represents an increasing proportion of the environmental management budget as surface waste units are closed. Therefore, a comprehensive evaluation of the monitoring program for eighteen regulated waste units was conducted to identify areas where monitoring could be optimized. The units evaluated varied considerably in the scope of monitoring; ranging from two wells to hundreds of wells. In order to systematically evaluate such disparate monitoring networks, SRS developed a decision-logic analysis using flow sheets to address potential areas of optimization. Five areas were identified for evaluation, including: (1) Comparison of current monitoring to regulatory requirements, (2) Spatial distribution, (3) Temporal sampling, (4) Analyte requirements, and (5) Reporting frequency and content. Optimization recommendations were made for fifteen of the eighteen groundwater units. The spatial evaluation resulted in recommendations to suspend sampling in 79 wells and add sampling at 16 wells. The temporal evaluation resulted in recommendations to reduce the number of well visits per year by 504. Analyte reductions were recommended at three groundwater units, with increases at three other units. Reporting frequency reductions were recommended for five units. Approximately $700,000 (direct dollars) of potential annualized cost savings were identified for these groundwater units, provided all recommendations are approved. The largest area of savings was associated with reducing the reporting frequency. The optimization approach has been presented to the EPA and South Carolina Department of Environmental Control (SCHDEC), with unit-specific recommendations approved for all five units

  19. 7 CFR 1753.6 - Standards, specifications, and general requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., software systems, and firmware financed with loan funds must be year 2000 compliant, as defined in 7 CFR... construction financed with loan funds must meet the standards and specifications established by RUS. 7 CFR part... compliant, as defined in 7 CFR 1735.22(e). (d) All materials and equipment financed with loan funds...

  20. 7 CFR 1753.6 - Standards, specifications, and general requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., software systems, and firmware financed with loan funds must be year 2000 compliant, as defined in 7 CFR... construction financed with loan funds must meet the standards and specifications established by RUS. 7 CFR part... compliant, as defined in 7 CFR 1735.22(e). (d) All materials and equipment financed with loan funds...

  1. 49 CFR 179.401-1 - Individual specification requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) § 179.400-5(d). Standard heat transfer rate. (Btu per day per lb. of water capacity, max.) (see § 179... restrictions § 179.400-20 179.400-20. Transfer line insulation § 179.400-17 Not required....

  2. Requirements and specifications of the space telescope for scientific operations

    NASA Technical Reports Server (NTRS)

    West, D. K.

    1976-01-01

    Requirements for the scientific operations of the Space Telescope and the Science Institute are used to develop operational interfaces between user scientists and the NASA ground system. General data systems are defined for observatory scheduling, daily science planning, and science data management. Hardware, software, manpower, and space are specified for several science institute locations and support options.

  3. 49 CFR 173.62 - Specific packaging requirements for explosives.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... paper, multiwall, water resistantplastics woven plastics Receptacles fibreboard metal plastics wood... wood, sift-proof Sheets paper, water resistant paper, waxed plastics woven plastics (5H1/2/3).paper.... PARTICULAR PACKING REQUIREMENTS OR EXCEPTIONS:1. The Intermediate packagings must be filled with...

  4. 40 CFR 86.1406 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) Emission Regulations for New Gasoline-Fueled Otto-Cycle Light-Duty Vehicles and New Gasoline-Fueled Otto-Cycle Light-Duty Trucks; Certification Short Test Procedures § 86.1406 Equipment required and... adjusted to the lowest available inertia weight setting and must meet the load speed...

  5. 40 CFR 86.1406 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) Emission Regulations for New Gasoline-Fueled Otto-Cycle Light-Duty Vehicles and New Gasoline-Fueled Otto-Cycle Light-Duty Trucks; Certification Short Test Procedures § 86.1406 Equipment required and... adjusted to the lowest available inertia weight setting and must meet the load speed...

  6. 40 CFR 86.1406 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) Emission Regulations for New Gasoline-Fueled Otto-Cycle Light-Duty Vehicles and New Gasoline-Fueled Otto-Cycle Light-Duty Trucks; Certification Short Test Procedures § 86.1406 Equipment required and... adjusted to the lowest available inertia weight setting and must meet the load speed...

  7. 40 CFR 86.1406 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) Emission Regulations for New Gasoline-Fueled Otto-Cycle Light-Duty Vehicles and New Gasoline-Fueled Otto-Cycle Light-Duty Trucks; Certification Short Test Procedures § 86.1406 Equipment required and... adjusted to the lowest available inertia weight setting and must meet the load speed...

  8. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... addition to the requirements found in ASME CSD-1 (incorporated by reference; see 46 CFR 63.05-1), the... than pressure controls, water columns, drains, and steam gages may be installed on the float chamber or... into the boiler through the float chamber. The boiler feed piping must comply with the...

  9. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... addition to the requirements found in ASME CSD-1 (incorporated by reference; see 46 CFR 63.05-1), the... than pressure controls, water columns, drains, and steam gages may be installed on the float chamber or... into the boiler through the float chamber. The boiler feed piping must comply with the...

  10. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... addition to the requirements found in ASME CSD-1 (incorporated by reference; see 46 CFR 63.05-1), the... than pressure controls, water columns, drains, and steam gages may be installed on the float chamber or... into the boiler through the float chamber. The boiler feed piping must comply with the...

  11. End User Acceptance - Requirements or Specifications, Certification, Testing

    NASA Technical Reports Server (NTRS)

    Jeevarajan, Judith

    2013-01-01

    NASA follows top level safety requirement of two-failure tolerance (t hree levels of controls or design for minimum risk) to all catastroph ic hazards in the design of safe li-ion batteries for space use. ? R igorous development testing at appropriate levels to credible offnominal conditions and review of test data. ? Implement robust design con trols based on test results and test again to confirm safety at the a ppropriate levels. ? Stringent testing of all (100%) flight batteries (from button cells to large batteries).

  12. Software requirements specification document for the AREST code development

    SciTech Connect

    Engel, D.W.; McGrail, B.P.; Whitney, P.D.; Gray, W.J.; Williford, R.E.; White, M.D.; Eslinger, P.W.; Altenhofen, M.K.

    1993-11-01

    The Analysis of the Repository Source Term (AREST) computer code was selected in 1992 by the U.S. Department of Energy. The AREST code will be used to analyze the performance of an underground high level nuclear waste repository. The AREST code is being modified by the Pacific Northwest Laboratory (PNL) in order to evaluate the engineered barrier and waste package designs, model regulatory compliance, analyze sensitivities, and support total systems performance assessment modeling. The current version of the AREST code was developed to be a very useful tool for analyzing model uncertainties and sensitivities to input parameters. The code has also been used successfully in supplying source-terms that were used in a total systems performance assessment. The current version, however, has been found to be inadequate for the comparison and selection of a design for the waste package. This is due to the assumptions and simplifications made in the selection of the process and system models. Thus, the new version of the AREST code will be designed to focus on the details of the individual processes and implementation of more realistic models. This document describes the requirements of the new models that will be implemented. Included in this document is a section describing the near-field environmental conditions for this waste package modeling, description of the new process models that will be implemented, and a description of the computer requirements for the new version of the AREST code.

  13. Repackaging SRS Black Box TRU Waste

    SciTech Connect

    Swale, D. J.; Stone, K.A.; Milner, T. N.

    2006-01-09

    Historically, large items of TRU Waste, which were too large to be packaged in drums for disposal have been packaged in various sizes of custom made plywood boxes at the Savannah River Site (SRS), for many years. These boxes were subsequently packaged into large steel ''Black Boxes'' for storage at SRS, pending availability of Characterization and Certification capability, to facilitate disposal of larger items of TRU Waste. There are approximately 107 Black Boxes in inventory at SRS, each measuring some 18' x 12' x 7', and weighing up to 45,000 lbs. These Black Boxes have been stored since the early 1980s. The project to repackage this waste into Standard Large Boxes (SLBs), Standard Waste Boxes (SWB) and Ten Drum Overpacks (TDOP), for subsequent characterization and WIPP disposal, commenced in FY04. To date, 10 Black Boxes have been repackaged, resulting in 40 SLB-2's, and 37 B25 overpack boxes, these B25's will be overpacked in SLB-2's prior to shipping to WIPP. This paper will describe experience to date from this project.

  14. PAMS photo image retrieval prototype system requirements specification

    SciTech Connect

    Conner, M.L.

    1996-04-30

    This project is part of the Photo Audiovisual Management System (PAMS). The project was initially identified in 1989 and has since been has been worked on under various names such as Image Retrieval and Viewing System, Photo Image Retrieval Subsystem and Image Processing and Compression System. This document builds upon the information collected and the analysis performed in the earlier phases of this project. The PAMS Photo Imaging subsystem will provide the means of capturing low resolution digital images from Photography`s negative files and associating the digital images with a record in the PAMS photo database. The digital images and key photo identification information will be accessible to HAN users to assist in locating and identifying specific photographs. After identifying desired photographs, users may request photo prints or high resolution digital images directly from Photography. The digital images captured by this project are for identification purposes only and are not intended to be of sufficient quality for subsequent use.

  15. Are patient specific meshes required for EIT head imaging?

    PubMed

    Jehl, Markus; Aristovich, Kirill; Faulkner, Mayo; Holder, David

    2016-06-01

    Head imaging with electrical impedance tomography (EIT) is usually done with time-differential measurements, to reduce time-invariant modelling errors. Previous research suggested that more accurate head models improved image quality, but no thorough analysis has been done on the required accuracy. We propose a novel pipeline for creation of precise head meshes from magnetic resonance imaging and computed tomography scans, which was applied to four different heads. Voltages were simulated on all four heads for perturbations of different magnitude, haemorrhage and ischaemia, in five different positions and for three levels of instrumentation noise. Statistical analysis showed that reconstructions on the correct mesh were on average 25% better than on the other meshes. However, the stroke detection rates were not improved. We conclude that a generic head mesh is sufficient for monitoring patients for secondary strokes following head trauma. PMID:27206049

  16. HSCT4.0 Application: Software Requirements Specification

    NASA Technical Reports Server (NTRS)

    Salas, A. O.; Walsh, J. L.; Mason, B. H.; Weston, R. P.; Townsend, J. C.; Samareh, J. A.; Green, L. L.

    2001-01-01

    The software requirements for the High Performance Computing and Communication Program High Speed Civil Transport application project, referred to as HSCT4.0, are described. The objective of the HSCT4.0 application project is to demonstrate the application of high-performance computing techniques to the problem of multidisciplinary design optimization of a supersonic transport configuration, using high-fidelity analysis simulations. Descriptions of the various functions (and the relationships among them) that make up the multidisciplinary application as well as the constraints on the software design arc provided. This document serves to establish an agreement between the suppliers and the customer as to what the HSCT4.0 application should do and provides to the software developers the information necessary to design and implement the system.

  17. Specification of advanced safety modeling requirements (Rev. 0).

    SciTech Connect

    Fanning, T. H.; Tautges, T. J.

    2008-06-30

    The U.S. Department of Energy's Global Nuclear Energy Partnership has lead to renewed interest in liquid-metal-cooled fast reactors for the purpose of closing the nuclear fuel cycle and making more efficient use of future repository capacity. However, the U.S. has not designed or constructed a fast reactor in nearly 30 years. Accurate, high-fidelity, whole-plant dynamics safety simulations will play a crucial role by providing confidence that component and system designs will satisfy established design limits and safety margins under a wide variety of operational, design basis, and beyond design basis transient conditions. Current modeling capabilities for fast reactor safety analyses have resulted from several hundred person-years of code development effort supported by experimental validation. The broad spectrum of mechanistic and phenomenological models that have been developed represent an enormous amount of institutional knowledge that needs to be maintained. Complicating this, the existing code architectures for safety modeling evolved from programming practices of the 1970s. This has lead to monolithic applications with interdependent data models which require significant knowledge of the complexities of the entire code in order for each component to be maintained. In order to develop an advanced fast reactor safety modeling capability, the limitations of the existing code architecture must be overcome while preserving the capabilities that already exist. To accomplish this, a set of advanced safety modeling requirements is defined, based on modern programming practices, that focuses on modular development within a flexible coupling framework. An approach for integrating the existing capabilities of the SAS4A/SASSYS-1 fast reactor safety analysis code into the SHARP framework is provided in order to preserve existing capabilities while providing a smooth transition to advanced modeling capabilities. In doing this, the advanced fast reactor safety models will

  18. System Requirements Toward Specification of an Image Recorder

    NASA Astrophysics Data System (ADS)

    Corsover, S. L.; Herzog, D. G.; Donze, D. W.

    1986-07-01

    This paper approaches the topic of Laser Beam Image Recording from the user's perspective. It discusses the parameters of concern to a system group charged with specifying a high performance Laser Beam Image Recording Subsystem. The paper concentrates on those parameters that have significant impact on interface complexity, image quality and cost. A block diagram of a typical Laser Beam Recorder is shown along with a brief explanation of the following subsystems: Image Data Interface, Control Data Interface, Image Data Processor, Annotation, Scan Data Processor, Command and Control, Electro Optics, Film Transport, and Built-In-Test Equipment. Ranges of the following signal parameters are given: Line Rate, Data Rate, Data Duty Factor and Bytes/Line. Typical values of the following critical image quality parameters are given: Resolution, Raster and Banding, Geometric Fidelity, and Density Fidelity. Each of the critical parameters are defined and measuring techniques and equipment discussed. A list of system level questions which a user should consider when formulating the Laser Beam Recorder specification are also presented.

  19. Robust Entrainment of Circadian Oscillators Requires Specific Phase Response Curves

    PubMed Central

    Pfeuty, Benjamin; Thommen, Quentin; Lefranc, Marc

    2011-01-01

    The circadian clocks keeping time in many living organisms rely on self-sustained biochemical oscillations entrained by external cues, such as light, to the 24-h cycle induced by Earth's rotation. However, environmental cues are unreliable due to the variability of habitats, weather conditions, or cue-sensing mechanisms among individuals. A tempting hypothesis is that circadian clocks have evolved so as to be robust to fluctuations in the signal that entrains them. To support this hypothesis, we analyze the synchronization behavior of weakly and periodically forced oscillators in terms of their phase response curve (PRC), which measures phase changes induced by a perturbation applied at different times of the cycle. We establish a general relationship between the robustness of key entrainment properties, such as stability and oscillator phase, on the one hand, and the shape of the PRC as characterized by a specific curvature or the existence of a dead zone, on the other hand. The criteria obtained are applied to computational models of circadian clocks and account for the disparate robustness properties of various forcing schemes. Finally, the analysis of PRCs measured experimentally in several organisms strongly suggests a case of convergent evolution toward an optimal strategy for maintaining a clock that is accurate and robust to environmental fluctuations. PMID:21641300

  20. Search Hanford accessible reports electronically system requirements specification

    SciTech Connect

    Willers, K.J.

    1994-02-28

    The Emergency, Safety, & Quality (ESQ) Services organization of Westinghouse Hanford Company (WHC) is experiencing changes related to new programs. The programs include Corrective Actions Management Systems, Self-Assessment, Trend Analysis, and Lessons Learned analysis. The programs are pushing up organizational costs while funding and manpower levels are projected to be dropping. A large cost involved in implementing the new programs is the cost for people to locate unstructured information required to make decisions or write documentation. But, most resources for retrieving information are found in structured database systems. This means that unstructured information that must be located has to be found by one of these methods: (1) searching manually through documents, (2) searching individual documents one at a time with word processing programs, (3) searching through text fields with query systems primarily designed for structured database information, or (4) develop a text search and retrieval system designed for unstructured information. One example of this is using Occurrence Reporting Processing System (ORPS) and Quality, Environmental, and Safety Tracking (QUEST) documents. An explanation of this is in the following textual box.

  1. Solar powered biohydrogen production requires specific localization of the hydrogenase

    SciTech Connect

    Burroughs, Nigel J.; Boehm, Marko; Eckert, Carrie; Mastroianni, Giulia; Spence, Edward M.; Yu, Jianfeng; Nixon, Peter J.; Appel, Jens; Mullineaux, Conrad W.; Bryan, Samantha J.

    2014-09-04

    Cyanobacteria contain a bidirectional [NiFe] hydrogenase which transiently produces hydrogen upon exposure of anoxic cells to light, potentially acting as a “valve” releasing excess electrons from the electron transport chain. However, its interaction with the photosynthetic electron transport chain remains unclear. By GFP-tagging the HoxF diaphorase subunit we show that the hydrogenase is thylakoid associated, comprising a population dispersed uniformly through the thylakoids and a subpopulation localized to discrete puncta in the distal thylakoid. Thylakoid localisation of both the HoxH and HoxY hydrogenase subunits is confirmed by immunogold electron microscopy. The diaphorase HoxE subunit is essential for recruitment to the dispersed thylakoid population, potentially anchoring the hydrogenase to the membrane, but aggregation to puncta occurs through a distinct HoxE-independent mechanism. Membrane association does not require NDH-1. Localization is dynamic on a scale of minutes, with anoxia and high light inducing a significant redistribution between these populations in favour of puncta. Lastly, since HoxE is essential for access to its electron donor, electron supply to the hydrogenase depends on a physiologically controlled localization, potentially offering a new avenue to enhance photosynthetic hydrogen production by exploiting localization/aggregation signals.

  2. Solar powered biohydrogen production requires specific localization of the hydrogenase

    DOE PAGESBeta

    Burroughs, Nigel J.; Boehm, Marko; Eckert, Carrie; Mastroianni, Giulia; Spence, Edward M.; Yu, Jianfeng; Nixon, Peter J.; Appel, Jens; Mullineaux, Conrad W.; Bryan, Samantha J.

    2014-09-04

    Cyanobacteria contain a bidirectional [NiFe] hydrogenase which transiently produces hydrogen upon exposure of anoxic cells to light, potentially acting as a “valve” releasing excess electrons from the electron transport chain. However, its interaction with the photosynthetic electron transport chain remains unclear. By GFP-tagging the HoxF diaphorase subunit we show that the hydrogenase is thylakoid associated, comprising a population dispersed uniformly through the thylakoids and a subpopulation localized to discrete puncta in the distal thylakoid. Thylakoid localisation of both the HoxH and HoxY hydrogenase subunits is confirmed by immunogold electron microscopy. The diaphorase HoxE subunit is essential for recruitment to themore » dispersed thylakoid population, potentially anchoring the hydrogenase to the membrane, but aggregation to puncta occurs through a distinct HoxE-independent mechanism. Membrane association does not require NDH-1. Localization is dynamic on a scale of minutes, with anoxia and high light inducing a significant redistribution between these populations in favour of puncta. Lastly, since HoxE is essential for access to its electron donor, electron supply to the hydrogenase depends on a physiologically controlled localization, potentially offering a new avenue to enhance photosynthetic hydrogen production by exploiting localization/aggregation signals.« less

  3. JET MIXING ANALYSIS FOR SRS HIGH-LEVEL WASTE RECOVERY

    SciTech Connect

    Lee, S.

    2011-07-05

    The process of recovering the waste in storage tanks at the Savannah River Site (SRS) typically requires mixing the contents of the tank to ensure uniformity of the discharge stream. Mixing is accomplished with one to four slurry pumps located within the tank liquid. The slurry pump may be fixed in position or they may rotate depending on the specific mixing requirements. The high-level waste in Tank 48 contains insoluble solids in the form of potassium tetraphenyl borate compounds (KTPB), monosodium titanate (MST), and sludge. Tank 48 is equipped with 4 slurry pumps, which are intended to suspend the insoluble solids prior to transfer of the waste to the Fluidized Bed Steam Reformer (FBSR) process. The FBSR process is being designed for a normal feed of 3.05 wt% insoluble solids. A chemical characterization study has shown the insoluble solids concentration is approximately 3.05 wt% when well-mixed. The project is requesting a Computational Fluid Dynamics (CFD) mixing study from SRNL to determine the solids behavior with 2, 3, and 4 slurry pumps in operation and an estimate of the insoluble solids concentration at the suction of the transfer pump to the FBSR process. The impact of cooling coils is not considered in the current work. The work consists of two principal objectives by taking a CFD approach: (1) To estimate insoluble solids concentration transferred from Tank 48 to the Waste Feed Tank in the FBSR process and (2) To assess the impact of different combinations of four slurry pumps on insoluble solids suspension and mixing in Tank 48. For this work, several different combinations of a maximum of four pumps are considered to determine the resulting flow patterns and local flow velocities which are thought to be associated with sludge particle mixing. Two different elevations of pump nozzles are used for an assessment of the flow patterns on the tank mixing. Pump design and operating parameters used for the analysis are summarized in Table 1. The baseline

  4. Summary of the SRS Severe Accident Analysis Program, 1987--1992

    SciTech Connect

    Long, T.A.; Hyder, M.L.; Britt, T.E.; Allison, D.K.; Chow, S.; Graves, R.D.; DeWald, A.B. Jr.; Monson, P.R. Jr.; Wooten, L.A.

    1992-11-01

    The Severe Accident Analysis Program (SAAP) is a program of experimental and analytical studies aimed at characterizing severe accidents that might occur in the Savannah River Site Production Reactors. The goals of the Severe Accident Analysis Program are: To develop an understanding of severe accidents in SRS reactors that is adequate to support safety documentation for these reactors, including the Safety Analysis Report (SAR), the Probabilistic Risk Assessment (PRA), and other studies evaluating the safety of reactor operation; To provide tools and bases for the evaluation of existing or proposed safety related equipment in the SRS reactors; To provide bases for the development of accident management procedures for the SRS reactors; To develop and maintain on the site a sufficient body of knowledge, including documents, computer codes, and cognizant engineers and scientists, that can be used to authoritatively resolve questions or issues related to reactor accidents. The Severe Accident Analysis Program was instituted in 1987 and has already produced a substantial amount of information, and specialized calculational tools. Products of the Severe Accident Analysis Program (listed in Section 9 of this report) have been used in the development of the Safety Analysis Report (SAR) and the Probabilistic Risk Assessment (PRA), and in the development of technical specifications for the SRS reactors. A staff of about seven people is currently involved directly in the program and in providing input on severe accidents to other SRS activities.

  5. Review of seismicity and ground motion studies related to development of seismic design at SRS

    SciTech Connect

    Stephenson, D.E.; Acree, J.R.

    1992-08-01

    The NRC response spectra developed in Reg. Guide 1.60 is being used in the studies related to restarting of the existing Savannah River Site (SRS) reactors. Because it envelopes all the other site specific spectra which have been developed for SRS, it provides significant conservatism in the design and analysis of the reactor systems for ground motions of this value or with these probability levels. This spectral shape is also the shape used for the design of the recently licensed Vogtle Nuclear Station, located south of the Savannah River from the SRS. This report provides a summary of the data base used to develop the design basis earthquake. This includes the seismicity, rates of occurrence, magnitudes, and attenuation relationships. A summary is provided for the studies performed and methodologies used to establish the design basis earthquake for SRS. The ground motion response spectra developed from the various studies are also summarized. The seismic hazard and PGA`s developed for other critical facilities in the region are discussed, and the SRS seismic instrumentation is presented. The programs for resolving outstanding issues are discussed and conclusions are presented.

  6. Srs2 mediates PCNA-SUMO-dependent inhibition of DNA repair synthesis

    PubMed Central

    Burkovics, Peter; Sebesta, Marek; Sisakova, Alexandra; Plault, Nicolas; Szukacsov, Valeria; Robert, Thomas; Pinter, Lajos; Marini, Victoria; Kolesar, Peter; Haracska, Lajos; Gangloff, Serge; Krejci, Lumir

    2013-01-01

    Completion of DNA replication needs to be ensured even when challenged with fork progression problems or DNA damage. PCNA and its modifications constitute a molecular switch to control distinct repair pathways. In yeast, SUMOylated PCNA (S-PCNA) recruits Srs2 to sites of replication where Srs2 can disrupt Rad51 filaments and prevent homologous recombination (HR). We report here an unexpected additional mechanism by which S-PCNA and Srs2 block the synthesis-dependent extension of a recombination intermediate, thus limiting its potentially hazardous resolution in association with a cross-over. This new Srs2 activity requires the SUMO interaction motif at its C-terminus, but neither its translocase activity nor its interaction with Rad51. Srs2 binding to S-PCNA dissociates Polδ and Polη from the repair synthesis machinery, thus revealing a novel regulatory mechanism controlling spontaneous genome rearrangements. Our results suggest that cycling cells use the Siz1-dependent SUMOylation of PCNA to limit the extension of repair synthesis during template switch or HR and attenuate reciprocal DNA strand exchanges to maintain genome stability. PMID:23395907

  7. Srs2 mediates PCNA-SUMO-dependent inhibition of DNA repair synthesis.

    PubMed

    Burkovics, Peter; Sebesta, Marek; Sisakova, Alexandra; Plault, Nicolas; Szukacsov, Valeria; Robert, Thomas; Pinter, Lajos; Marini, Victoria; Kolesar, Peter; Haracska, Lajos; Gangloff, Serge; Krejci, Lumir

    2013-03-01

    Completion of DNA replication needs to be ensured even when challenged with fork progression problems or DNA damage. PCNA and its modifications constitute a molecular switch to control distinct repair pathways. In yeast, SUMOylated PCNA (S-PCNA) recruits Srs2 to sites of replication where Srs2 can disrupt Rad51 filaments and prevent homologous recombination (HR). We report here an unexpected additional mechanism by which S-PCNA and Srs2 block the synthesis-dependent extension of a recombination intermediate, thus limiting its potentially hazardous resolution in association with a cross-over. This new Srs2 activity requires the SUMO interaction motif at its C-terminus, but neither its translocase activity nor its interaction with Rad51. Srs2 binding to S-PCNA dissociates Polδ and Polη from the repair synthesis machinery, thus revealing a novel regulatory mechanism controlling spontaneous genome rearrangements. Our results suggest that cycling cells use the Siz1-dependent SUMOylation of PCNA to limit the extension of repair synthesis during template switch or HR and attenuate reciprocal DNA strand exchanges to maintain genome stability. PMID:23395907

  8. Dismantlement and decontamination of a plutonium-238 facility at SRS

    SciTech Connect

    Smith, R.H. Jr.; Hootman, H.E.

    1994-01-01

    There has been very little, documented decontamination and decommissioning (D&D) experience on which to project cleanup costs and schedules for plutonium facilities at SRS and other DOE sites. A portion of the HB-Line, a plutonium-238 processing facility at SRS, has been undergoing D&D intermittently since 1984. Although this cleanup effort was not originally intended to quantify results, some key data have been project has demonstrated effective methods of accumulated, and the performing D&D work, and has demonstrated cleanup equipment and techniques under conditions of high contamination. Plutonium facilities where D&D is already underway provide an opportunity for` timely field testing of characterization, size reduction, and decontamination techniques. Some data are presented here; however, more specific tests and data may be obtained during the remainder of this project. This project has been recommended as a candidate test facility for a DOE planned ``Integrated D&D Demonstration`` managed by EM-50 to develop and demonstrate technology for D&D and surplus facilities deactivation. Both the remainder of this project and the Integrated D&D Demonstration Program can benefit from a joint effort, and the, overall costs should be reduced.

  9. 10 CFR 40.32 - General requirements for issuance of specific licenses.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false General requirements for issuance of specific licenses. 40.32 Section 40.32 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE MATERIAL License Applications § 40.32 General requirements for issuance of specific licenses. An application for a specific license will be approved if: (a)...

  10. 10 CFR 40.34 - Special requirements for issuance of specific licenses.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Special requirements for issuance of specific licenses. 40.34 Section 40.34 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE MATERIAL License Applications § 40.34 Special requirements for issuance of specific licenses. (a) An application for a specific license to manufacture...

  11. 40 CFR 166.32 - Reporting and recordkeeping requirements for specific, quarantine, and public health exemptions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... requirements for specific, quarantine, and public health exemptions. 166.32 Section 166.32 Protection of... AGENCIES FOR USE OF PESTICIDES UNDER EMERGENCY CONDITIONS Specific, Quarantine, and Public Health Exemptions § 166.32 Reporting and recordkeeping requirements for specific, quarantine, and public...

  12. 40 CFR 166.32 - Reporting and recordkeeping requirements for specific, quarantine, and public health exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requirements for specific, quarantine, and public health exemptions. 166.32 Section 166.32 Protection of... AGENCIES FOR USE OF PESTICIDES UNDER EMERGENCY CONDITIONS Specific, Quarantine, and Public Health Exemptions § 166.32 Reporting and recordkeeping requirements for specific, quarantine, and public...

  13. 40 CFR 166.32 - Reporting and recordkeeping requirements for specific, quarantine, and public health exemptions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requirements for specific, quarantine, and public health exemptions. 166.32 Section 166.32 Protection of... AGENCIES FOR USE OF PESTICIDES UNDER EMERGENCY CONDITIONS Specific, Quarantine, and Public Health Exemptions § 166.32 Reporting and recordkeeping requirements for specific, quarantine, and public...

  14. 40 CFR 166.32 - Reporting and recordkeeping requirements for specific, quarantine, and public health exemptions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... requirements for specific, quarantine, and public health exemptions. 166.32 Section 166.32 Protection of... AGENCIES FOR USE OF PESTICIDES UNDER EMERGENCY CONDITIONS Specific, Quarantine, and Public Health Exemptions § 166.32 Reporting and recordkeeping requirements for specific, quarantine, and public...

  15. Thermodynamic Modeling of the SRS Evaporators: Part II. The 3H System

    SciTech Connect

    Jantzen, C.M.

    2001-10-02

    Accumulations of two solid phases have formed scale deposits in the Savannah River Site 2H Evaporator system since late 1996. The aluminosilicate scale deposits caused the evaporator pot to become inoperable in October 1999. Accumulations of the diuranate phase have caused criticality concerns in the SRS 2H Evaporator. In order to ensure that similar deposits are not and will not form in the SRS 3H Evaporator, thermodynamically derived activity diagrams specific to the feeds processed from Tanks 30 and 32 are evaluated in this report.

  16. Pilot study risk assessment for selected problems at the Savannah River Site (SRS)

    SciTech Connect

    Hamilton, L.D.; Holtzman, S.; Meinhold, A.; Morris, S.C.; Pardi, R.; Sun, C. ); Daniels, J.I.; Layton, D.; McKone, T.E.; Straume, T.; Anspaugh, L. )

    1993-03-01

    An assessment of the health risks was made for releases of tritium and [sup 137]Cs from the Savannah River Site (SRS) at water-receptor locations downriver. Although reactor operations were shut down at the SRS in 1989, liquid wastes continue to be released to the Savannah River either by direct discharges into onsite surface waters or by groundwater transport into surface waters from waste facilities. Existing state mandates will cause the liquid waste streams from future operations to go directly into surface waters. Two drinking water processing plants take water from the river approximately 129 km downriver from the SRS. Potential incremental risks of cancer fatality to individuals and each population were analyzed for either no further reactor operations or resumption of operation of one specific reactor.

  17. Pilot study risk assessment for selected problems at the Savannah River Site (SRS)

    SciTech Connect

    Hamilton, L.D.; Holtzman, S.; Meinhold, A.; Morris, S.C.; Pardi, R.; Sun, C.; Daniels, J.I.; Layton, D.; McKone, T.E.; Straume, T.; Anspaugh, L.

    1993-03-01

    An assessment of the health risks was made for releases of tritium and {sup 137}Cs from the Savannah River Site (SRS) at water-receptor locations downriver. Although reactor operations were shut down at the SRS in 1989, liquid wastes continue to be released to the Savannah River either by direct discharges into onsite surface waters or by groundwater transport into surface waters from waste facilities. Existing state mandates will cause the liquid waste streams from future operations to go directly into surface waters. Two drinking water processing plants take water from the river approximately 129 km downriver from the SRS. Potential incremental risks of cancer fatality to individuals and each population were analyzed for either no further reactor operations or resumption of operation of one specific reactor.

  18. Methodology for Estimating Ingestion Dose for Emergency Response at SRS

    SciTech Connect

    Simpkins, A.A.

    2002-04-22

    At the Savannah River Site (SRS), emergency response models estimate dose for inhalation and ground shine pathways. A methodology has been developed to incorporate ingestion doses into the emergency response models. The methodology follows a two-phase approach. The first phase estimates site-specific derived response levels (DRLs) which can be compared with predicted ground-level concentrations to determine if intervention is needed to protect the public. This phase uses accepted methods with little deviation from recommended guidance. The second phase uses site-specific data to estimate a 'best estimate' dose to offsite individuals from ingestion of foodstuffs. While this method deviates from recommended guidance, it is technically defensibly and more realistic. As guidance is updated, these methods also will need to be updated.

  19. Input to the PRAST computer code used in the SRS probabilistic risk assessment

    SciTech Connect

    Kearnaghan, D.P.

    1992-10-15

    The PRAST (Production Reactor Algorithm for Source Terms) computer code was developed by Westinghouse Savannah River Company and Science Application International Corporation for the quantification of source terms for the SRS Savannah River Site (SRS) Reactor Probabilistic Risk Assessment. PRAST requires as input a set of release fractions, decontamination factors, transfer fractions and source term characteristics that accurately reflect the conditions that are evaluated by PRAST. This document links the analyses which form the basis for the PRAST input parameters. In addition, it gives the distribution of the input parameters that are uncertain and considered to be important to the evaluation of the source terms to the environment.

  20. Improved Methodology Application for 12-Rad Analysis in a Shielded Facility at SRS

    SciTech Connect

    Paul, P.

    2003-01-31

    The DOE Order 420.1 requires establishing 12-rad evacuation zone boundaries and installing Criticality Accident Alarm System (CAAS) per ANS-8.3 standard for facilities having a probability of criticality greater than 10-6 per year. The H-Canyon at the Savannah River Site (SRS) is one of the reprocessing facilities where SRS reactor fuels, research reactor fuels, and other fissile materials are processed and purified using a modified Purex process called H-Modified or HM Process. This paper discusses an improved methodology for 12-rad zone analysis and its implementation within this large shielded facility that has a large variety of criticality sources and scenarios.

  1. Reference manual for a Requirements Specification Language (RSL), version 2.0

    NASA Technical Reports Server (NTRS)

    Fisher, Gene L.; Cohen, Gerald C.

    1993-01-01

    This report is a Reference Manual for a general-purpose Requirements Specification Language, RSL. The purpose of RSL is to specify precisely the external structure of a mechanized system and to define requirements that the system must meet. A system can be comprised of a mixture of hardware, software, and human processing elements. RSL is a hybrid of features found in several popular requirements specification languages and includes constructs for formal mathematical specification.

  2. Functions and Requirements and Specifications for Replacement of the Computer Automated Surveillance System (CASS)

    SciTech Connect

    SCAIEF, C.C.

    1999-12-16

    This functions, requirements and specifications document defines the baseline requirements and criteria for the design, purchase, fabrication, construction, installation, and operation of the system to replace the Computer Automated Surveillance System (CASS) alarm monitoring.

  3. SRS stainless steel beneficial reuse program

    SciTech Connect

    Boettinger, W.L.

    1997-02-01

    The US Department of Energy`s (DOE) Savannah River Site (SRS) has thousands of tons of stainless steel radioactive scrap metal (RSNI). Much of the metal is volumetrically contaminated. There is no {open_quotes}de minimis{close_quotes} free release level for volumetric material, and therefore no way to recycle the metal into the normal commercial market. If declared waste, the metal would qualify as low level radioactive waste (LLW) and ultimately be dispositioned through shallow land buried at a cost of millions of dollars. The metal however could be recycled in a {open_quotes}controlled release{close_quote} manner, in the form of containers to hold other types of radioactive waste. This form of recycle is generally referred to as {open_quotes}Beneficial Reuse{close_quotes}. Beneficial reuse reduces the amount of disposal space needed and reduces the need for virgin containers which would themselves become contaminated. Stainless steel is particularly suited for long term storage because of its resistance to corrosion. To assess the practicality of stainless steel RSM recycle the SRS Benficial Reuse Program began a demonstration in 1994, funded by the DOE Office of Science and Technology. This paper discusses the experiences gained in this program.

  4. SRS baseline hydrogeologic investigation: Summary report

    SciTech Connect

    Bledsoe, H.W.; Aadland, R.K. ); Sargent, K.A. . Dept. of Geology)

    1990-11-01

    Work on the Savannah River Site (SRS) Baseline Hydrogeologic Investigation began in 1983 when it was determined that the knowledge of the plant hydrogeologic systems needed to be expanded and improved in response to changing stratigraphic and hydrostratigraphic terminology and increased involvement by regulatory agencies (Bledsoe, 1984). Additionally, site-wide data were needed to determine flow paths, gradients, and velocities associated with the different aquifers underlying the plant site. The program was divided into three phases in order to allow the results of one phase to be evaluated and necessary changes and improvements incorporated into the following phases. This report summarizes the results of all three phases and includes modified graphic logs, lithologic descriptions of the different geologic formations, profiles of each cluster site, hydrostratigraphic cross sections, hydrographs of selected wells within each cluster for the first full year of uninterrupted water level measurements, potentiometric maps developed from data collected from all clusters, completion diagrams for each well, and a summary of laboratory tests. Additionally, the proposed new classification of hydrostratigraphic units at SRS (Aadland and Bledsoe, 1990) has been incorporated.

  5. CH2M Hill Hanford Group, Inc. Standards and Requirements Identification Document (SRID) Requirements Management System and Requirements Specification

    SciTech Connect

    JOHNSON, A.L.

    2000-11-30

    The current Tank Farm Contractor (TFC) for the U. S. Department of Energy, Office of River Protection (ORP), River Protection Project (RPP), CH2M Hill Hanford Group, Inc. (CHG), will use a computer based requirements management system. The system will serve as a tool to assist in identifying, capturing, and maintaining the Standards/Requirements Identification Document (S/RID) requirements and links to implementing procedures and other documents. By managing requirements as one integrated set, CHG will be able to carry out its mission more efficiently and effectively. CHG has chosen the Dynamic Object Oriented Requirements System (DOORS{trademark}) as the preferred computer based requirements management system. Accordingly, the S/RID program will use DOORS{trademark}. DOORS{trademark} will replace the Environmental Requirements Management Interface (ERMI) system as the tool for S/RID data management. The DOORS{trademark} S/RID test project currently resides on the DOORSTM test server. The S/RID project will be migrated to the DOORS{trademark} production server. After the migration the S/RID project will be considered a production project and will no longer reside on the test server.

  6. Assessing autistic traits in a Taiwan preschool population: cross-cultural validation of the Social Responsiveness Scale (SRS).

    PubMed

    Wang, Jessica; Lee, Li-Ching; Chen, Ying-Sheue; Hsu, Ju-Wei

    2012-11-01

    The cross-cultural validity of the Mandarin-adaptation of the social responsiveness scale (SRS) was examined in a sample of N = 307 participants in Taiwan, 140 typically developing and 167 with clinically-diagnosed developmental disorders. This scale is an autism assessment tool that provides a quantitative rather than categorical measure of social impairment in the general population. SRS total and subscale scores distinguished significantly between autism spectrum disorder and other developmental disorders (p < 0.01). Total SRS scores and sensitivity and specificity of the scale for diagnosing developmental disorders in the Taiwan study were similar to those observed in Western studies. These findings support the cross-cultural validity of the SRS scale for detecting autistic traits and for distinguishing between autism and other neuropsychiatric conditions. PMID:22407579

  7. Development of a Rotary Microfilter for SRS HLW Applications

    SciTech Connect

    MICHAEL, POIRIER

    2004-11-24

    The processing rate of Savannah River Site high level waste decontamination processes are limited by the flow rate of the solid-liquid separation. The baseline process, using a 0.1 micron cross flow filter, produces 0.02 gpm/ft2 of filtrate under expected operating conditions. Savannah River National Laboratory personnel identified the rotary microfilter as a technology that could significantly increase filter flux, with throughput improvements of as much as 10X for that specific operation. With funding from the Department of Energy Office of Cleanup Technologies, SRNL personnel are evaluating and developing the rotary microfilter for radioactive service at SRS. This work includes pilot-scale and actual waste testing to evaluate system reliability, the impact of radiation on system components, the filter flux for a variety of waste streams, and relative performance for alternative filter media.

  8. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  9. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  10. 40 CFR 60.2095 - What site-specific documentation is required?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false What site-specific documentation is... Training and Qualification § 60.2095 What site-specific documentation is required? (a) Documentation must... required under §§ 60.2055 through 60.2065. (9) Procedures for handling ash. (10) A list of the...

  11. 78 FR 47015 - Software Requirement Specifications for Digital Computer Software Used in Safety Systems of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-02

    ... issued with a temporary identification as Draft Regulatory Guide, DG-1209 on August 22, 2012 (77 FR 50726... COMMISSION Software Requirement Specifications for Digital Computer Software Used in Safety Systems of... 1 of RG 1.172, ``Software Requirement Specifications for Digital Computer Software used in...

  12. 40 CFR 62.14620 - What site-specific documentation is required?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 8 2011-07-01 2011-07-01 false What site-specific documentation is... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That... site-specific documentation is required? (a) Documentation must be available at the facility...

  13. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  14. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  15. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  16. Tools reference manual for a Requirements Specification Language (RSL), version 2.0

    NASA Technical Reports Server (NTRS)

    Fisher, Gene L.; Cohen, Gerald C.

    1993-01-01

    This report describes a general-purpose Requirements Specification Language, RSL. The purpose of RSL is to specify precisely the external structure of a mechanized system and to define requirements that the system must meet. A system can be comprised of a mixture of hardware, software, and human processing elements. RSL is a hybrid of features found in several popular requirements specification languages, such as SADT (Structured Analysis and Design Technique), PSL (Problem Statement Language), and RMF (Requirements Modeling Framework). While languages such as these have useful features for structuring a specification, they generally lack formality. To overcome the deficiencies of informal requirements languages, RSL has constructs for formal mathematical specification. These constructs are similar to those found in formal specification languages such as EHDM (Enhanced Hierarchical Development Methodology), Larch, and OBJ3.

  17. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201 Individual specification requirements applicable to non-pressure tank car tanks....

  18. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.101 Individual specification requirements applicable to pressure tank...

  19. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.301 Individual specification requirements for multi-unit tank car tanks. (a) In addition...

  20. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.301 Individual specification requirements for multi-unit tank car tanks. (a) In addition...

  1. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201 Individual specification requirements applicable to non-pressure tank car tanks....

  2. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.101 Individual specification requirements applicable to pressure tank...

  3. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.101 Individual specification requirements applicable to pressure tank...

  4. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.101 Individual specification requirements applicable to pressure tank...

  5. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.301 Individual specification requirements for multi-unit tank car tanks. (a) In addition...

  6. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201 Individual specification requirements applicable to non-pressure tank car tanks....

  7. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201 Individual specification requirements applicable to non-pressure tank car tanks....

  8. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.301 Individual specification requirements for multi-unit tank car tanks. (a) In addition...

  9. Status of The Indian SRS Indus-2

    SciTech Connect

    Sahni, V. C.

    2009-07-07

    Raja Ramanna Centre for Advanced Technology (RRCAT, formerly called Centre for Advanced Technology) is a prime R and D laboratory of Indian Department of Atomic Energy devoted to developing technologies related to accelerators and lasers as well as their applications. RRCAT is home to 2 synchrotron radiation sources (SRS): Indus-1 (a 100 mA, 450 MeV storage ring) and Indus-2 (a 2.5 GeV booster cum storage ring designed for a current of up to 300 mA), sharing a common injector system, comprising of 20 MeV microtron and 450-700 MeV range booster synchrotron. Most of the accelerator hardware has been built indigenously. Normally beam is injected into Indus-2 (and accumulated) at 550 MeV, and ramped to 2 or 2.5 GeV depending on the user needs. At present we have permission from Atomic Energy Regulatory Board (Indian agency charged with radiation protection responsibility in the country) to operate Indus-2 at 2.5 GeV with up to 50 mA and in the next stage we will get authorization to go up to 100 mA. Currently 5 beam lines on Indus-1 and 3 on Indus-2 are operational and work is going on 4 more beam lines on Indus-2 and is progressing well. The 3 completed beam lines on Indus-2 are: high resolution XRD, position sensitive detector based multi channel EXAFS (Extended X-ray Absorption Fine Structure) and EDXRD (Energy Dispersive X-ray Diffraction). The paper gives an overview of how the SRS program at RRCAT has evolved over the years, where we stand today and also some of our future plans.

  10. 49 CFR 173.301a - Additional general requirements for shipment of specification cylinders.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... REGULATIONS SHIPPERS-GENERAL REQUIREMENTS FOR SHIPMENTS AND PACKAGINGS Gases; Preparation and Packaging § 173.301a Additional general requirements for shipment of specification cylinders. (a) General. The... 49 Transportation 2 2013-10-01 2013-10-01 false Additional general requirements for shipment...

  11. Tandemization of a Subregion of the Enhancer Sequences from SRS 19-6 Murine Leukemia Virus Associated with T-Lymphoid but Not Other Leukemias

    PubMed Central

    Granger, Steven W.; Bundy, Linda M.; Fan, Hung

    1999-01-01

    Most simple retroviruses induce tumors of a single cell type when infected into susceptible hosts. The SRS 19-6 murine leukemia virus (MuLV), which originated in mainland China, induces leukemias of multiple cellular origins. Indeed, infected mice often harbor more than one tumor type. Since the enhancers of many MuLVs are major determinants of tumor specificity, we tested the role of the SRS 19-6 MuLV enhancers in its broad disease specificity. The enhancer elements of the Moloney MuLV (M-MuLV) were replaced by the 170-bp enhancers of SRS 19-6 MuLV, yielding the recombinants ΔMo+SRS+ and ΔMo+SRS− M-MuLV. M-MuLV normally induces T-lymphoid tumors in all infected mice. Surprisingly, when neonatal mice were inoculated with ΔMo+SRS+ or ΔMo+SRS− M-MuLV, all tumors were of T-lymphoid origin, typical of M-MuLV rather than SRS 19-6 MuLV. Thus, the SRS 19-6 MuLV enhancers did not confer the broad disease specificity of SRS 19-6 MuLV to M-MuLV. However, all tumors contained ΔMo+SRS M-MuLV proviruses with common enhancer alterations. These alterations consisted of tandem multimerization of a subregion of the SRS 19-6 enhancers, encompassing the conserved LVb and core sites and adjacent sequences. Moreover, when tumors induced by the parental SRS 19-6 MuLV were analyzed, most of the T-lymphoid tumors had similar enhancer alterations in the same region whereas tumors of other lineages retained the parental SRS 19-6 MuLV enhancers. These results emphasize the importance of a subregion of the SRS 19-6 MuLV enhancer in induction of T-cell lymphoma. The relevant sequences were consistent with crucial sequences for T-cell lymphomagenesis identified for other MuLVs such as M-MuLV and SL3-3 MuLV. These results also suggest that other regions of the SRS 19-6 MuLV genome contribute to its broad leukemogenic spectrum. PMID:10438804

  12. Significance of Soft Zone Sediments at the SRS

    SciTech Connect

    Aadland, R.K.

    2000-02-03

    The purpose of this report is to provide information on the origin, extent and stability of ''soft zones'' in the carbonate bearing strata at the Savannah River Site (SRS). As part of this study, a comprehensive historical compendium of how soft zones have been addressed during the past 47 years at SRS is reviewed.

  13. American College of Radiology (ACR) and American Society for Radiation Oncology (ASTRO) Practice Guideline for the Performance of Stereotactic Radiosurgery (SRS)

    PubMed Central

    Seung, Steven K.; Larson, David A.; Galvin, James M.; Mehta, Minesh P.; Potters, Louis; Schultz, Christopher J.; Yajnik, Santosh V.; Hartford, Alan C.; Rosenthal, Seth A.

    2014-01-01

    American College of Radiology and American Society for Radiation Oncology Practice Guideline for the Performance of Stereotactic Radiosurgery (SRS). SRS is a safe and efficacious treatment option of a variety of benign and malignant disorders involving intracranial structures and selected extracranial lesions. SRS involves a high dose of ionizing radiation with a high degree of precision and spatial accuracy. A quality SRS program requires a multidisciplinary team involved in the patient management. Organization, appropriate staffing, and careful adherence to detail and to established SRS standards is important to ensure operational efficiency and to improve the likelihood of procedural success. A collaborative effort of the American College of Radiology and American Society for Therapeutic Radiation Oncology has produced a practice guideline for SRS. The guideline defines the qualifications and responsibilities of all the involved personnel, including the radiation oncologist, neurosurgeon, and qualified medical physicist. Quality assurance is essential for safe and accurate delivery of treatment with SRS. Quality assurance issues for the treatment unit, stereotactic accessories, medical imaging, and treatment-planning system are presented and discussed. Adherence to these practice guidelines can be part of ensuring quality and patient safety in a successful SRS program. PMID:23681017

  14. Mrc1 and Srs2 are major actors in the regulation of spontaneous crossover

    PubMed Central

    Robert, Thomas; Dervins, Delphine; Fabre, Francis; Gangloff, Serge

    2006-01-01

    In vegetative cells, most recombination intermediates are metabolized without an association with a crossover (CO). The avoidance of COs allows for repair and prevents genomic rearrangements, potentially deleterious if the sequences involved are at ectopic locations. We have designed a system that permits to screen spontaneous intragenic recombination events in Saccharomyces cerevisiae and to investigate the CO outcome in different genetic contexts. We have analyzed the CO outcome in the absence of the Srs2 and Sgs1 helicases, DNA damage checkpoint proteins as well as in a mutant proliferating cell nuclear antigen (PCNA) and found that they all contribute to genome stability. Remarkably high effects on COs are mediated by srs2Δ, mrc1Δ and a pol30-RR mutation in PCNA. Our results support the view that Mrc1 plays a specific role in DNA replication, promoting the Srs2 recruitment to PCNA independently of checkpoint signaling. Srs2 would prevent formation of double Holliday junctions (dHJs) and thus CO formation. Sgs1 also negatively regulates CO formation but through a different process that resolves dHJs to yield non-CO products. PMID:16724109

  15. Engineering Specification for Large-aperture UVO Space Telescopes Derived from Science Requirements

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip; Arnold, William; Bevan, Ryan M.; Smith, W. Scott.; Kirk, Charles S.; Postman, Mark

    2013-01-01

    An advanced large aperture UV/optical UVO space telescope is required for the next generation of astrophysics and exoplanet science. The science requirements of proposed exoplanet and astrophysics missions were used to determine the encircled energy, point spread function stability and thermal environment requirements. These requirements then determine the optical wavefront specification for potential telescope assemblies which can fit inside current and planned launch vehicles. The optical wavefront specification becomes the top level of the error budget that is split into various sources that control the structural, thermal and optical design.

  16. 41 CFR 301-2.5 - What travel arrangements require specific authorization or prior approval?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... a specific authorization or prior approval for: (a) Use of other than coach-class service on common... attendance at a conference; and (p) Due to an employee's medical requirements or religious beliefs,...

  17. 40 CFR 60.3019 - What site-specific documentation is required?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Model Rule-Operator Training and Qualification § 60.3019 What site-specific documentation is required... air supply levels. (5) Procedures for operating the incinerator and associated air pollution...

  18. 40 CFR 60.3019 - What site-specific documentation is required?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Model Rule-Operator Training and Qualification § 60.3019 What site-specific documentation is required... air supply levels. (5) Procedures for operating the incinerator and associated air pollution...

  19. 40 CFR 60.3019 - What site-specific documentation is required?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Model Rule-Operator Training and Qualification § 60.3019 What site-specific documentation is required... air supply levels. (5) Procedures for operating the incinerator and associated air pollution...

  20. 40 CFR 60.3019 - What site-specific documentation is required?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Model Rule-Operator Training and Qualification § 60.3019 What site-specific documentation is required... air supply levels. (5) Procedures for operating the incinerator and associated air pollution...

  1. AMTD: Update of Engineering Specifications Derived from Science Requirements for Future UVOIR Space Telescopes

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip

    2014-01-01

    AMTD is using a Science Driven Systems Engineering approach to develop Engineering Specifications based on Science Measurement Requirements and Implementation Constraints. Science requirements meet the needs of both Exoplanet and General Astrophysics science. Engineering Specifications are guiding our effort to mature to TRL-6 the critical technologies needed to produce 4-m or larger flight-qualified UVOIR mirrors by 2018 so that a viable mission can be considered by the 2020 Decadal Review.

  2. 40 CFR 270.18 - Specific part B information requirements for waste piles.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Specific part B information requirements for waste piles. 270.18 Section 270.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) EPA ADMINISTERED PERMIT PROGRAMS: THE HAZARDOUS WASTE PERMIT PROGRAM Permit Application § 270.18 Specific part...

  3. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... specification tank cars. 180.509 Section 180.509 Transportation Other Regulations Relating to Transportation...) CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.509 Requirements for inspection and test of specification tank cars. (a) General. Each tank car owner must...

  4. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... specification tank cars. 180.509 Section 180.509 Transportation Other Regulations Relating to Transportation...) CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.509 Requirements for inspection and test of specification tank cars. (a) General. Each tank car owner must...

  5. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Individual specification requirements for multi-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS FOR TANK...

  6. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Individual specification requirements applicable to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS...

  7. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Individual specification requirements applicable to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS...

  8. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Plans and specifications required for new construction... new construction. (a) General. (1) Specifications. (2) General Arrangement Plan of decks, holds, inner... facilities for all crewmembers. (i) Navigation bridge visibility. For vessels of 100 meters (328 feet)...

  9. 40 CFR 270.19 - Specific part B information requirements for incinerators.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Specific part B information requirements for incinerators. 270.19 Section 270.19 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) EPA ADMINISTERED PERMIT PROGRAMS: THE HAZARDOUS WASTE PERMIT PROGRAM Permit Application § 270.19 Specific part...

  10. 46 CFR 164.009-3 - Noncombustible materials not requiring specific approval.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Noncombustible materials not requiring specific approval. 164.009-3 Section 164.009-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL MATERIALS Noncombustible Materials for Merchant Vessels § 164.009-3...

  11. 7 CFR 1724.54 - Requirements for RUS approval of plans and specifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the application. (See 7 CFR part 1710, subpart F.) Prior to issuing the plans and specifications for... DESIGN POLICIES AND PROCEDURES Electric System Design § 1724.54 Requirements for RUS approval of plans... specifications for transmission construction projects which are not based on RUS approved line design data or...

  12. 40 CFR 60.2095 - What site-specific documentation is required?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false What site-specific documentation is... site-specific documentation is required? (a) Documentation must be available at the facility and....2055 through 60.2065. (9) Procedures for handling ash. (10) A list of the wastes burned during...

  13. 40 CFR 62.14620 - What site-specific documentation is required?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 9 2014-07-01 2014-07-01 false What site-specific documentation is... site-specific documentation is required? (a) Documentation must be available at the facility and....14580 through 62.14590. (9) Procedures for handling ash. (10) A list of the wastes burned during...

  14. 40 CFR 60.2095 - What site-specific documentation is required?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false What site-specific documentation is... site-specific documentation is required? (a) Documentation must be available at the facility and....2055 through 60.2065. (9) Procedures for handling ash. (10) A list of the wastes burned during...

  15. 40 CFR 62.14620 - What site-specific documentation is required?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 9 2012-07-01 2012-07-01 false What site-specific documentation is... site-specific documentation is required? (a) Documentation must be available at the facility and....14580 through 62.14590. (9) Procedures for handling ash. (10) A list of the wastes burned during...

  16. 40 CFR 62.14620 - What site-specific documentation is required?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 9 2013-07-01 2013-07-01 false What site-specific documentation is... site-specific documentation is required? (a) Documentation must be available at the facility and....14580 through 62.14590. (9) Procedures for handling ash. (10) A list of the wastes burned during...

  17. 40 CFR 270.25 - Specific part B information requirements for equipment.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Specific part B information requirements for equipment. 270.25 Section 270.25 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) EPA ADMINISTERED PERMIT PROGRAMS: THE HAZARDOUS WASTE PERMIT PROGRAM Permit Application § 270.25 Specific part...

  18. 40 CFR 60.2095 - What site-specific documentation is required?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Commercial and Industrial Solid Waste Incineration Units for Which Construction Is Commenced After November... 40 Protection of Environment 6 2011-07-01 2011-07-01 false What site-specific documentation is... Training and Qualification § 60.2095 What site-specific documentation is required? (a) Documentation...

  19. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Specific requirements for spent fuel storage cask approval and fabrication. 72.236 Section 72.236 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND...

  20. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Specific requirements for spent fuel storage cask approval and fabrication. 72.236 Section 72.236 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND...

  1. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Specific requirements for spent fuel storage cask approval and fabrication. 72.236 Section 72.236 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE...

  2. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 2 2013-01-01 2013-01-01 false Specific requirements for spent fuel storage cask approval and fabrication. 72.236 Section 72.236 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND...

  3. 40 CFR 270.18 - Specific part B information requirements for waste piles.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requirements for waste piles. 270.18 Section 270.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) EPA ADMINISTERED PERMIT PROGRAMS: THE HAZARDOUS WASTE PERMIT PROGRAM Permit Application § 270.18 Specific part B information requirements for waste piles. Except as...

  4. 40 CFR 270.17 - Specific part B information requirements for surface impoundments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requirements for surface impoundments. 270.17 Section 270.17 Protection of Environment ENVIRONMENTAL PROTECTION... PROGRAM Permit Application § 270.17 Specific part B information requirements for surface impoundments... of hazardous waste in surface impoundments must provide the following additional information: (a)...

  5. 40 CFR 270.17 - Specific part B information requirements for surface impoundments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requirements for surface impoundments. 270.17 Section 270.17 Protection of Environment ENVIRONMENTAL PROTECTION... PROGRAM Permit Application § 270.17 Specific part B information requirements for surface impoundments... of hazardous waste in surface impoundments must provide the following additional information: (a)...

  6. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE Approval of Spent Fuel Storage Casks § 72.236 Specific requirements for... storage cask, maximum heat designed to be dissipated, maximum spent fuel loading limit, condition of...

  7. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... HOUSING AND URBAN DEVELOPMENT GENERAL INTRODUCTION TO FHA PROGRAMS Minimum Property Standards § 200.936 Supplementary specific procedural requirements under HUD building products certification program for solid...

  8. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... HOUSING AND URBAN DEVELOPMENT GENERAL INTRODUCTION TO FHA PROGRAMS Minimum Property Standards § 200.936 Supplementary specific procedural requirements under HUD building products certification program for solid...

  9. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... HOUSING AND URBAN DEVELOPMENT GENERAL INTRODUCTION TO FHA PROGRAMS Minimum Property Standards § 200.936 Supplementary specific procedural requirements under HUD building products certification program for solid...

  10. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... HOUSING AND URBAN DEVELOPMENT GENERAL INTRODUCTION TO FHA PROGRAMS Minimum Property Standards § 200.936 Supplementary specific procedural requirements under HUD building products certification program for solid...

  11. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... HOUSING AND URBAN DEVELOPMENT GENERAL INTRODUCTION TO FHA PROGRAMS Minimum Property Standards § 200.936 Supplementary specific procedural requirements under HUD building products certification program for solid...

  12. 49 CFR 180.205 - General requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false General requirements for requalification of....205 General requirements for requalification of specification cylinders. (a) General. Each cylinder... for steel and nickel cylinders (IBR, see § 171.7 of this subchapter); C-6.1 for seamless...

  13. 49 CFR 180.205 - General requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false General requirements for requalification of....205 General requirements for requalification of specification cylinders. (a) General. Each cylinder... for steel and nickel cylinders (IBR, see § 171.7 of this subchapter); C-6.1 for seamless...

  14. 49 CFR 180.205 - General requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false General requirements for requalification of....205 General requirements for requalification of specification cylinders. (a) General. Each cylinder... for steel and nickel cylinders (IBR, see § 171.7 of this subchapter); C-6.1 for seamless...

  15. Treated effluent disposal system process control computer software requirements and specification

    SciTech Connect

    Graf, F.A. Jr.

    1994-06-03

    The software requirements for the monitor and control system that will be associated with the effluent collection pipeline system known as the 200 Area Treated Effluent Disposal System is covered. The control logic for the two pump stations and specific requirements for the graphic displays are detailed.

  16. Operational Safety Requirements and Operating Specification Documentation compliance instrumentation matrices: 200 East Area Tank Farms

    SciTech Connect

    Story, D.R.

    1995-03-01

    This document contains information about matrices complied of instrumentation used to comply with the existing Operational Safety Requirements from Safety Analysis Reports and Operating, Specification Documentation requirements for 200 East Area Tank Farms. These matrices contain the primary instrumentation needed to comply with each OSR and/or OSD requirement as well as any backup instrumentation that may be used should the primary device be out of service. The referenced matrices are provided as attachments to this document.

  17. TRANSFORMING THE SRS ENVIRONMENTAL BUSINESS: COMMUNICATION AND APPLIED PROJECT MANAGEMENT PRINCIPLES

    SciTech Connect

    Saldivar, E.

    2010-01-20

    A process for communicating information relating to core business functions that also encourages improving internal communications has been established at SRS. This process continues to grow and strengthen as the multiple Contractors, Regulators and DOE-SR relationships mature. A number of management communication tools have been initiated, retooled, rebooted or continued with enhancements to ensure appropriate information is communicated to all levels with environmental responsibility at SRS. The types of information that are the focus of this improved process are feedback from the customer and from informational exchange forums (i.e., Challenge Opportunity and Resolution (COR), SRS Regulatory Integration Team (SRIT), Environmental Quality Management Division (EQMD), Senior Environmental Managers Council (SEMC), etc.). These forums, SRS environmental functions centralization, and the creation of a Regulatory Integration process allows for cross-functional decision making, problem solving and information sharing that involves the field organizations, Environmental Compliance Authorities (ECA), Subject Matter Experts (SME), DOE and the Regulators. Numerous examples of effective decision-making and problem solving will be shared. Lessons Learned involving inadequate communications and the resulting impacts on the environment, customer satisfaction, and relationships will also be discussed. Additionally, the focus on improved communications also includes maintaining awareness of business activities. The tools being utilized to facilitate the continuing improvement of internal communications include weekly staff meetings for all individuals within the organization, quarterly ECA and SME meeting, quarterly Regulatory Integration & Environmental Services (RI&ES) All-Hands meetings hosted by the Director, bi-weekly EQMD and EQMD Lite meetings with the customer, bi-annual SRIT meetings, and COR meetings on an as need basis. In addition, an existing Required Reading Program

  18. CEMENTITIOUS GROUT FOR CLOSING SRS HIGH LEVEL WASTE TANKS - #12315

    SciTech Connect

    Langton, C.; Burns, H.; Stefanko, D.

    2012-01-10

    In 1997, the first two United States Department of Energy (US DOE) high level waste tanks (Tanks 17-F and 20-F: Type IV, single shell tanks) were taken out of service (permanently closed) at the Savannah River Site (SRS). In 2012, the DOE plans to remove from service two additional Savannah River Site (SRS) Type IV high-level waste tanks, Tanks 18-F and 19-F. These tanks were constructed in the late 1950's and received low-heat waste and do not contain cooling coils. Operational closure of Tanks 18-F and 19-F is intended to be consistent with the applicable requirements of the Resource Conservation and Recovery Act (RCRA) and the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) and will be performed in accordance with South Carolina Department of Health and Environmental Control (SCDHEC). The closure will physically stabilize two 4.92E+04 cubic meter (1.3 E+06 gallon) carbon steel tanks and isolate and stabilize any residual contaminants left in the tanks. The closure will also fill, physically stabilize and isolate ancillary equipment abandoned in the tanks. A Performance Assessment (PA) has been developed to assess the long-term fate and transport of residual contamination in the environment resulting from the operational closure of the F-Area Tank Farm (FTF) waste tanks. Next generation flowable, zero-bleed cementitious grouts were designed, tested, and specified for closing Tanks 18-F and 19-F and for filling the abandoned equipment. Fill requirements were developed for both the tank and equipment grouts. All grout formulations were required to be alkaline with a pH of 12.4 and chemically reduction potential (Eh) of -200 to -400 to stabilize selected potential contaminants of concern. This was achieved by including Portland cement and Grade 100 slag in the mixes, respectively. Ingredients and proportions of cementitious reagents were selected and adjusted, respectively, to support the mass placement strategy developed by closure

  19. U.S. Space Station Freedom Supplemental Reboost System (SRS) design and operational impacts

    NASA Astrophysics Data System (ADS)

    Winters, Brian A.; Winters, Donna M.

    1992-07-01

    Current designs of the U.S. Space Station Freedom Propulsion System require the capability to perform reboost, attitude control, and other orbit adjustment maneuvers as part of normal operations. In order to reduce the quantity of hydrazine consumed by the Primary Propulsion System, the propulsion system includes the capability to propulsively dispose waste gases that are generated by various station systems and experiments. The impulses generated by the Supplemental Reboost System (SRS) are used to offset orbital decay due to atmospheric drag and reduce propellant requirements. This paper presents the designs of the SRS including schematics of the waste gas collection architecture, various system and component characteristics, and methods of system operation. Estimates of waste gas quantities, propellant savings, and operational altitude impacts are provided.

  20. An introduction to requirements capture using PVS: Specification of a simple autopilot

    NASA Technical Reports Server (NTRS)

    Butler, Ricky W.

    1996-01-01

    This paper presents an introduction to capturing software requirements in the PVS formal language. The object of study is a simplified digital autopilot that was motivated in part by the mode control panel of NASA Langley's Boeing 737 research aircraft. The paper first presents the requirements for this autopilot in English and then steps the reader through a translation of these requirements into formal mathematics. Along the way deficiencies in the English specification are noted and repaired. Once completed, the formal PVS requirement is analyzed using the PVS theorem prover and shown to maintain an invariant over its state space.

  1. 75 FR 32158 - Information Collection; SRS Publications Evaluation Card

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ...In accordance with the Paperwork Reduction Act of 1995, the Forest Service is seeking comments from all interested individuals and organizations on the extension, with no revision, of a currently approved information collection, SRS Publications Evaluation...

  2. Towards the formal specification of the requirements and design of a processor interface unit: HOL listings

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1993-01-01

    This technical report contains the HOL listings of the specification of the design and major portions of the requirements for a commercially developed processor interface unit (or PIU). The PIU is an interface chip performing memory interface, bus interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance-free operation, or both. This report contains the actual HOL listings of the PIU specification as it currently exists. Section two of this report contains general-purpose HOL theories that support the PIU specification. These theories include definitions for the hardware components used in the PIU, our implementation of bit words, and our implementation of temporal logic. Section three contains the HOL listings for the PIU design specification. Aside from the PIU internal bus (I-Bus), this specification is complete. Section four contains the HOL listings for a major portion of the PIU requirements specification. Specifically, it contains most of the definition for the PIU behavior associated with memory accesses initiated by the local processor.

  3. A fast shutdown system for SRS (Savannah River Site) reactors

    SciTech Connect

    Baumann, N.P.

    1990-01-01

    Power has been sharply reduced at Savannah River Site (SRS) reactors in large part to ensure that no bulk boiling occurs during hypothesized loss of coolant accidents. A fast shutdown system is essential to regain much of this lost power. Computations and experiments indicate that a He-3 injection system will serve this function. Instrumented tests of a full system are planned for early 1991 for one of the SRS reactors. 4 refs., 7 figs., 1 tab.

  4. A Methodology for Writing High Quality Requirements Specification and Evaluating Existing Ones

    NASA Technical Reports Server (NTRS)

    Rosenberg, Linda; Hammer, Theodore

    1999-01-01

    Requirements development and management have always been critical in the implementation of software systems; engineers are unable to build what analysts can't define. It is generally accepted that the earlier in the life cycle potential risks are identified the easier it is to eliminate or manage the conditions that introduce that risk. Problems that are not found until testing are approximately 14 times more costly to fix than if the problem was found in the requirement phase. The requirements specification, as the first tangible representation of the capability to be produced, establishes the basis for all of the project's engineering management and assurance functions. If the quality of the requirements specification is poor it can give rise to risks in all areas of the project. Recently, automated tools have become available to support requirements management. The use of these tools not only provides support in the definition and tracing of requirements, but it also opens the door to effective use of metrics in characterizing and assessing the quality of the requirement specifications.

  5. A Methodology for Writing High Quality Requirement Specifications and for Evaluating Existing Ones

    NASA Technical Reports Server (NTRS)

    Rosenberg, Linda; Hammer, Theodore

    1999-01-01

    Requirements development and management have always been critical in the implementation of software systems-engineers are unable to build what analysts can not define. It is generally accepted that the earlier in the life cycle potential risks are identified the easier it is to eliminate or manage the conditions that introduce that risk. Problems that are not found until testing are approximately 14 times more costly to fix than if the problem was found in the requirement phase. The requirements specification, as the first tangible representation of the capability to be produced, establishes the basis for all of the project's engineering management and assurance functions. If the quality of the requirements specification is poor it can give rise to risks in all areas of the project. Recently, automated tools have become available to support requirements management. The use of these tools not only provides support in the definition and tracing of requirements, but it also opens the door to effective use of metrics in characterizing and assessing the quality of the requirement specifications.

  6. Discrete Notch signaling requirements in the specification of hematopoietic stem cells

    PubMed Central

    Kim, Albert D; Melick, Chase H; Clements, Wilson K; Stachura, David L; Distel, Martin; Panáková, Daniela; MacRae, Calum; Mork, Lindsey A; Crump, J Gage; Traver, David

    2014-01-01

    Hematopoietic stem cells (HSCs) require multiple molecular inputs for proper specification, including activity of the Notch signaling pathway. A requirement for the Notch1 and dispensability of the Notch2 receptor has been demonstrated in mice, but the role of the remaining Notch receptors has not been investigated. Here, we demonstrate that three of the four Notch receptors are independently required for the specification of HSCs in the zebrafish. The orthologues of the murine Notch1 receptor, Notch1a and Notch1b, are each required intrinsically to fate HSCs, just prior to their emergence from aortic hemogenic endothelium. By contrast, the Notch3 receptor is required earlier within the developing somite to regulate HSC emergence in a non-cell-autonomous manner. Epistatic analyses demonstrate that Notch3 function lies downstream of Wnt16, which is required for HSC specification through its regulation of two Notch ligands, dlc and dld. Collectively, these findings demonstrate for the first time that multiple Notch signaling inputs are required to specify HSCs and that Notch3 performs a novel role within the somite to regulate the neighboring precursors of hemogenic endothelium. PMID:25230933

  7. Cementitious Grout for Closing SRS High Level Waste Tanks - 12315

    SciTech Connect

    Langton, C.A.; Stefanko, D.B.; Burns, H.H.; Waymer, J.; Mhyre, W.B.; Herbert, J.E.; Jolly, J.C. Jr.

    2012-07-01

    In 1997, the first two United States Department of Energy (US DOE) high level waste tanks (Tanks 17-F and 20-F: Type IV, single shell tanks) were taken out of service (permanently closed) at the Savannah River Site (SRS). In 2012, the DOE plans to remove from service two additional Savannah River Site (SRS) Type IV high-level waste tanks, Tanks 18-F and 19-F. These tanks were constructed in the late 1950's and received low-heat waste and do not contain cooling coils. Operational closure of Tanks 18-F and 19-F is intended to be consistent with the applicable requirements of the Resource Conservation and Recovery Act (RCRA) and the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) and will be performed in accordance with South Carolina Department of Health and Environmental Control (SCDHEC). The closure will physically stabilize two 4.92E+04 cubic meter (1.3 E+06 gallon) carbon steel tanks and isolate and stabilize any residual contaminants left in the tanks. Ancillary equipment abandoned in the tanks will also be filled to the extent practical. A Performance Assessment (PA) has been developed to assess the long-term fate and transport of residual contamination in the environment resulting from the operational closure of the F-Area Tank Farm (FTF) waste tanks. Next generation flowable, zero-bleed cementitious grouts were designed, tested, and specified for closing Tanks 18-F and 19-F and for filling the abandoned equipment. Fill requirements were developed for both the tank and equipment grouts. All grout formulations were required to be alkaline with a pH of 12.4 and to be chemically reducing with a reduction potential (Eh) of -200 to -400. Grouts with this chemistry stabilize potential contaminants of concern. This was achieved by including Portland cement and Grade 100 slag in the mixes, respectively. Ingredients and proportions of cementitious reagents were selected and adjusted to support the mass placement strategy developed by

  8. Computer software requirements specification for the world model light duty utility arm system

    SciTech Connect

    Ellis, J.E.

    1996-02-01

    This Computer Software Requirements Specification defines the software requirements for the world model of the Light Duty Utility Arm (LDUA) System. It is intended to be used to guide the design of the application software, to be a basis for assessing the application software design, and to establish what is to be tested in the finished application software product. (This deploys end effectors into underground storage tanks by means of robotic arm on end of telescoping mast.)

  9. ALTERNATE APPROACH TO HAZARD CATEGORIZATION FOR SALTSTONE FACILITY AT SRS

    SciTech Connect

    Roy, B.

    2009-04-28

    The Saltstone Facility at Savannah River Site (SRS) was originally segmented into two segments: the Saltstone Production Facility (SPF) and the Saltstone Disposal Facility (SDF). Based on the inventory of radionuclides available for release the SPF and SDF were categorized as Nonreactor Hazard Category (HC)-3. The hazard categorization recognized the SDF will contain contributions of radionuclides which would exceed the HC-2 Threshold Quantity (TQ) in the form of grout. However it was determined not to impact the facility hazard categorization based on the grout being in a solid, monolithic form which was not easily dispersible. But, the impact of a quantity of unset grout expected to be present at the vault following operation of the process was not addressed. A Potential Inadequacy in Safety Analysis (PISA) was later issued based on the hazard categorization determination for the facility not addressing unset grout. This initiated a re-evaluation of the accident scenarios within the hazards analysis. During this re-evaluation, the segmentation of the facility was challenged based on the potential interaction between facility segments; specifically, the leachate return line and the grout transfer line, which were considered separate segments, are located in close proximity at one point. such that for certain events (NPH as well as External Vehicle Impact) both could be damaged simultaneously and spill contents on the ground that could commingle. This would violate the guideline for segmentation. Therefore, the Hazard Categorization (HC) was reevaluated based on the facility being a single segment and including the additional unset grout as part of total inventory. This total inventory far exceeded the limit for HC-2 TQ and made the facility's initial categorization as HC-2. However, alternative analysis methodology based on credible release fractions allowed in DOE-STD-1027-92 (Ref.1) showed that the Saltstone facility could still be categorized as Hazard Category

  10. Repeat Courses of Stereotactic Radiosurgery (SRS), Deferring Whole-Brain Irradiation, for New Brain Metastases After Initial SRS

    SciTech Connect

    Shultz, David B.; Modlin, Leslie A.; Jayachandran, Priya; Von Eyben, Rie; Gibbs, Iris C.; Choi, Clara Y.H.; Chang, Steven D.; Harsh, Griffith R.; Li, Gordon; Adler, John R.; Hancock, Steven L.; Soltys, Scott G.

    2015-08-01

    Purpose: To report the outcomes of repeat stereotactic radiosurgery (SRS), deferring whole-brain radiation therapy (WBRT), for distant intracranial recurrences and identify factors associated with prolonged overall survival (OS). Patients and Methods: We retrospectively identified 652 metastases in 95 patients treated with 2 or more courses of SRS for brain metastases, deferring WBRT. Cox regression analyzed factors predictive for OS. Results: Patients had a median of 2 metastases (range, 1-14) treated per course, with a median of 2 courses (range, 2-14) of SRS per patient. With a median follow-up after first SRS of 15 months (range, 3-98 months), the median OS from the time of the first and second course of SRS was 18 (95% confidence interval [CI] 15-24) and 11 months (95% CI 6-17), respectively. On multivariate analysis, histology, graded prognostic assessment score, aggregate tumor volume (but not number of metastases), and performance status correlated with OS. The 1-year cumulative incidence, with death as a competing risk, of local failure was 5% (95% CI 4-8%). Eighteen (24%) of 75 deaths were from neurologic causes. Nineteen patients (20%) eventually received WBRT. Adverse radiation events developed in 2% of SRS sites. Conclusion: Multiple courses of SRS, deferring WBRT, for distant brain metastases after initial SRS, seem to be a safe and effective approach. The graded prognostic assessment score, updated at each course, and aggregate tumor volume may help select patients in whom the deferral of WBRT might be most beneficial.

  11. Treatment of SRS Tank 48H Simulants Using Fenton's Reagent

    SciTech Connect

    Taylor, PA

    2003-11-18

    High-level-waste Tank 48H at the Savannah River Site (SRS) contains about 50,000 lb of tetraphenylborate (TPB), which must be destroyed to return the tank to active service. Laboratory-scale tests were conducted to evaluate the use of Fenton's Reagent (hydrogen peroxide and a metal catalyst) to treat simulants of the Tank 48H waste. Samples of the treated slurry and the off-gas were analyzed to determine the reaction products. Process parameters developed earlier by AEA Technology were used for these tests; namely (for 500 mL of waste simulant), reduce pH to 7.5 with nitric acid, heat to boiling, add hydrogen peroxide at 1 mL/min for 1 h, reduce pH to 3.5, and add the remaining peroxide at 2 mL/min. These parameters were developed to minimize the formation of tarry materials during the early part of the reaction and to minimize the concentration of total organic carbon in the final treated slurry. The treated samples contained low concentrations of total organic carbon (TOC) and no detectable TPB. Tests using a mixture of iron and copper salts as the Fenton's catalyst had a lower TOC concentration in the final treated slurry than did tests that used a copper-only catalyst. TPB is known to hydrolyze to benzene, particularly at high temperature and low pH, and copper is known to increase the rate of hydrolysis. Significant amounts of benzene were present in the off-gas from the tests, especially during the early portion of the treatment, indicating that the hydrolysis reaction was occurring in parallel with the oxidation of the TPB by Fenton's reagent. For the reaction conditions used in these tests, approximately equal fractions of the TPB were converted to benzene and carbon dioxide. Minimizing the formation of benzene is important to SRS personnel; however, this consideration was not addressed in the AEA-recommended parameters, since they did not analyze for benzene in the off-gas. Smaller amounts of carbon monoxide and other organics were also produced. One test

  12. ENVIRONMENTAL ASSESSMENT FOR THE NPDES STORM WATER COMPLIANCE ALTERNATIVES AT THE SRS

    SciTech Connect

    Shedrow, C

    2006-11-01

    The U.S. Department of Energy (DOE) prepared this environmental assessment (EA) to evaluate the potential environmental impacts associated with proposed and alternative actions to achieve water quality permit compliance at 38 storm water outfalls located at the Savannah River Site (SRS) (Figure 1-1). Effluent monitoring data indicates that some of these outfalls may not presently comply with new National Pollutant Discharge Elimination System (NPDES) Storm Water General Permit effluent standards that became effective July 1, 2005 (SCR000000). The NPDES permit requires that best management practices (BMPs) be implemented and maintained, as necessary, to ensure that storm water discharges at SRS do not cause or contribute to the contravention of applicable state water quality standards (WQS).

  13. Benefits of a Unified LaSRS++ Simulation for NAS-Wide and High-Fidelity Modeling

    NASA Technical Reports Server (NTRS)

    Glaab, Patricia; Madden, Michael

    2014-01-01

    The LaSRS++ high-fidelity vehicle simulation was extended in 2012 to support a NAS-wide simulation mode. Since the initial proof-of-concept, the LaSRS++ NAS-wide simulation is maturing into a research-ready tool. A primary benefit of this new capability is the consolidation of the two modeling paradigms under a single framework to save cost, facilitate iterative concept testing between the two tools, and to promote communication and model sharing between user communities at Langley. Specific benefits of each type of modeling are discussed along with the expected benefits of the unified framework. Current capability details of the LaSRS++ NAS-wide simulations are provided, including the visualization tool, live data interface, trajectory generators, terminal routing for arrivals and departures, maneuvering, re-routing, navigation, winds, and turbulence. The plan for future development is also described.

  14. Formal specification of requirements for analytical redundancy-based fault-tolerant flight control systems

    NASA Astrophysics Data System (ADS)

    Del Gobbo, Diego

    2000-10-01

    Flight control systems are undergoing a rapid process of automation. The use of Fly-By-Wire digital flight control systems in commercial aviation (Airbus 320 and Boeing FBW-B777) is a clear sign of this trend. The increased automation goes in parallel with an increased complexity of flight control systems with obvious consequences on reliability and safety. Flight control systems must meet strict fault-tolerance requirements. The standard solution to achieving fault tolerance capability relies on multi-string architectures. On the other hand, multi-string architectures further increase the complexity of the system inducing a reduction of overall reliability. In the past two decades a variety of techniques based on analytical redundancy have been suggested for fault diagnosis purposes. While research on analytical redundancy has obtained desirable results, a design methodology involving requirements specification and feasibility analysis of analytical redundancy based fault tolerant flight control systems is missing. The main objective of this research work is to describe within a formal framework the implications of adopting analytical redundancy as a basis to achieve fault tolerance. The research activity involves analysis of the analytical redundancy approach, analysis of flight control system informal requirements, and re-engineering (modeling and specification) of the fault tolerance requirements. The USAF military specification MIL-F-9490D and supporting documents are adopted as source for the flight control informal requirements. The De Havilland DHC-2 general aviation aircraft equipped with standard autopilot control functions is adopted as pilot application. Relational algebra is adopted as formal framework for the specification of the requirements. The detailed analysis and formalization of the requirements resulted in a better definition of the fault tolerance problem in the framework of analytical redundancy. Fault tolerance requirements and related

  15. 33 CFR 149.615 - What construction drawings and specifications are required?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false What construction drawings and... and Equipment General § 149.615 What construction drawings and specifications are required? (a) To... accepted Certifying Entity (CE) at least three copies of: (1) Each construction drawing and...

  16. 40 CFR 63.1103 - Source category-specific applicability, definitions, and requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 11 2013-07-01 2013-07-01 false Source category-specific applicability, definitions, and requirements. 63.1103 Section 63.1103 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES...

  17. 40 CFR 270.20 - Specific part B information requirements for land treatment facilities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Specific part B information requirements for land treatment facilities. 270.20 Section 270.20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) EPA ADMINISTERED PERMIT PROGRAMS: THE HAZARDOUS WASTE PERMIT PROGRAM Permit Application §...

  18. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Requirements for inspection and test of specification tank cars. 180.509 Section 180.509 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS CONTINUING QUALIFICATION...

  19. 77 FR 50726 - Software Requirement Specifications for Digital Computer Software and Complex Electronics Used in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ...The U.S. Nuclear Regulatory Commission (NRC or the Commission) is issuing for public comment draft regulatory guide (DG), DG-1209, ``Software Requirement Specifications for Digital Computer Software and Complex Electronics used in Safety Systems of Nuclear Power Plants.'' The DG-1209 is proposed Revision 1 of RG 1.172, dated September 1997. This revision endorses, with clarifications, the......

  20. 33 CFR 149.615 - What construction drawings and specifications are required?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false What construction drawings and... and Equipment General § 149.615 What construction drawings and specifications are required? (a) To... accepted Certifying Entity (CE) at least three copies of: (1) Each construction drawing and...

  1. 33 CFR 149.615 - What construction drawings and specifications are required?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false What construction drawings and... and Equipment General § 149.615 What construction drawings and specifications are required? (a) To... accepted Certifying Entity (CE) at least three copies of: (1) Each construction drawing and...

  2. 33 CFR 149.615 - What construction drawings and specifications are required?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false What construction drawings and... and Equipment General § 149.615 What construction drawings and specifications are required? (a) To... accepted Certifying Entity (CE) at least three copies of: (1) Each construction drawing and...

  3. 40 CFR 60.3019 - What site-specific documentation is required?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... procedures. (8) The waste management plan required under §§ 60.3010 through 60.3012. (9) Procedures for... Times for Other Solid Waste Incineration Units That Commenced Construction On or Before December 9, 2004 Model Rule-Operator Training and Qualification § 60.3019 What site-specific documentation is...

  4. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. It is available from the U.S. Department of... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for...

  5. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. It is available from the U.S. Department of... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for...

  6. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. It is available from the U.S. Department of... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for...

  7. 7 CFR 1724.54 - Requirements for RUS approval of plans and specifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... the application. (See 7 CFR part 1710, subpart F.) Prior to issuing the plans and specifications for...) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND PROCEDURES Electric System Design § 1724.54 Requirements for RUS approval of...

  8. 7 CFR 1724.54 - Requirements for RUS approval of plans and specifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... the application. (See 7 CFR part 1710, subpart F.) Prior to issuing the plans and specifications for...) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND PROCEDURES Electric System Design § 1724.54 Requirements for RUS approval of...

  9. 7 CFR 1724.54 - Requirements for RUS approval of plans and specifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... the application. (See 7 CFR part 1710, subpart F.) Prior to issuing the plans and specifications for...) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND PROCEDURES Electric System Design § 1724.54 Requirements for RUS approval of...

  10. A Skills Map: The General and Specific Competencies Required in Different Occupations. Progress Report.

    ERIC Educational Resources Information Center

    Gottfredson, Linda S.

    To aid in assessing the employability of individuals and of various groups of individuals in different types of occupations, the general abilities and specific skills required of workers in different occupations were studied. Data on a comprehensive set of occupations were obtained from the Dictionary of Occupational Titles (DOT) job ratings and…

  11. Educational Specifications and User Requirements for Elementary (K-6) Schools. SEF Report E1.

    ERIC Educational Resources Information Center

    Metropolitan Toronto School Board (Ontario). Study of Educational Facilities.

    Designed to be a working guideline for educators developing academic specifications and user requirements for the K-6 schools in metropolitan Toronto, this report has three major divisions. Part 1 contains material on the aims and objectives of education, the teaching-learning process, curriculum, and current educational trends. Part 2 relates the…

  12. Educational Specifications and User Requirements for Intermediate Schools. SEF E2.

    ERIC Educational Resources Information Center

    Metropolitan Toronto School Board (Ontario). Study of Educational Facilities.

    Educational specifications and user requirements for all types of intermediate schools (the middle school, the senior public school, and junior high schools) are presented. The essential purpose of this report is to provide educators and architects with general guidelines for designing improved school facilities. Part 1 focuses on the early…

  13. 24 CFR 200.944 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Supplementary specific requirements under the HUD building product standards and certification program for plywood and other performance rated wood-based structural-use panels. 200.944 Section 200.944 Housing and Urban Development Regulations Relating to Housing and Urban...

  14. 49 CFR 178.320 - General requirements applicable to all DOT specification cargo tank motor vehicles.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false General requirements applicable to all DOT specification cargo tank motor vehicles. 178.320 Section 178.320 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS...

  15. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Supplementary specific procedural requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section 200.937 Housing and Urban...

  16. 24 CFR 200.952 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Supplementary specific requirements under the HUD building product standards and certification program for particleboard interior stair treads. 200.952 Section 200.952 Housing and Urban Development Regulations Relating to Housing and...

  17. 49 CFR 173.301a - Additional general requirements for shipment of specification cylinders.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 2 2014-10-01 2014-10-01 false Additional general requirements for shipment of specification cylinders. 173.301a Section 173.301a Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS...

  18. 10 CFR 72.75 - Reporting requirements for specific events and conditions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 2 2013-01-01 2013-01-01 false Reporting requirements for specific events and conditions... events and conditions. (a) Emergency notifications: Each licensee shall notify the NRC Headquarters... possible but not later than four hours after the discovery of any of the following events or...

  19. 10 CFR 72.75 - Reporting requirements for specific events and conditions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Reporting requirements for specific events and conditions... events and conditions. (a) Emergency notifications: Each licensee shall notify the NRC Headquarters... possible but not later than four hours after the discovery of any of the following events or...

  20. 10 CFR 72.75 - Reporting requirements for specific events and conditions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 2 2012-01-01 2012-01-01 false Reporting requirements for specific events and conditions... events and conditions. (a) Emergency notifications: Each licensee shall notify the NRC Headquarters... possible but not later than four hours after the discovery of any of the following events or...

  1. 10 CFR 72.75 - Reporting requirements for specific events and conditions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Reporting requirements for specific events and conditions... events and conditions. (a) Emergency notifications: Each licensee shall notify the NRC Headquarters... possible but not later than four hours after the discovery of any of the following events or...

  2. 10 CFR 72.75 - Reporting requirements for specific events and conditions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Reporting requirements for specific events and conditions... events and conditions. (a) Emergency notifications: Each licensee shall notify the NRC Headquarters... possible but not later than four hours after the discovery of any of the following events or...

  3. 49 CFR 180.205 - General requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false General requirements for requalification of specification cylinders. 180.205 Section 180.205 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS CONTINUING QUALIFICATION...

  4. 40 CFR 166.32 - Reporting and recordkeeping requirements for specific, quarantine, and public health exemptions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Reporting and recordkeeping requirements for specific, quarantine, and public health exemptions. 166.32 Section 166.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS EXEMPTION OF FEDERAL AND STATE AGENCIES FOR USE OF PESTICIDES UNDER...

  5. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 6 2013-07-01 2013-07-01 false Demonstration of compliance with design specifications and manufacturing and test requirements. 53.51 Section 53.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) AMBIENT AIR MONITORING REFERENCE AND EQUIVALENT METHODS Procedures for...

  6. PILOT SCALE TESTING OF MONOSODIUM TITANATE MIXING FOR THE SRS SMALL COLUMN ION EXCHANGE PROCESS - 11224

    SciTech Connect

    Poirier, M.; Restivo, M.; Williams, M.; Herman, D.; Steeper, T.

    2011-01-25

    The Small Column Ion Exchange (SCIX) process is being developed to remove cesium, strontium, and select actinides from Savannah River Site (SRS) Liquid Waste using an existing waste tank (i.e., Tank 41H) to house the process. Savannah River National Laboratory (SRNL) is conducting pilot-scale mixing tests to determine the pump requirements for suspending monosodium titanate (MST), crystalline silicotitanate (CST), and simulated sludge. The purpose of this pilot scale testing is to determine the requirements for the pumps to suspend the MST particles so that they can contact the strontium and actinides in the liquid and be removed from the tank. The pilot-scale tank is a 1/10.85 linear scaled model of SRS Tank 41H. The tank diameter, tank liquid level, pump nozzle diameter, pump elevation, and cooling coil diameter are all 1/10.85 of their dimensions in Tank 41H. The pump locations correspond to the proposed locations in Tank 41H by the SCIX program (Risers B5 and B2 for two pump configurations and Risers B5, B3, and B1 for three pump configurations). The conclusions from this work follow: (i) Neither two standard slurry pumps nor two quad volute slurry pumps will provide sufficient power to initially suspend MST in an SRS waste tank. (ii) Two Submersible Mixer Pumps (SMPs) will provide sufficient power to initially suspend MST in an SRS waste tank. However, the testing shows the required pump discharge velocity is close to the maximum discharge velocity of the pump (within 12%). (iii) Three SMPs will provide sufficient power to initially suspend MST in an SRS waste tank. The testing shows the required pump discharge velocity is 66% of the maximum discharge velocity of the pump. (iv) Three SMPs are needed to resuspend MST that has settled in a waste tank at nominal 45 C for four weeks. The testing shows the required pump discharge velocity is 77% of the maximum discharge velocity of the pump. Two SMPs are not sufficient to resuspend MST that settled under these

  7. International Low Impact Docking System (iLIDS) Project Technical Requirements Specification, Revision F

    NASA Technical Reports Server (NTRS)

    Lewis, James L.

    2011-01-01

    The NASA Docking System (NDS) is NASA's implementation for the emerging International Docking System Standard (IDSS) using low impact docking technology. The NASA Docking System Project (NDSP) is the International Space Station (ISS) Program's project to produce the NDS, Common Docking Adapter (CDA) and Docking Hub. The NDS design evolved from the Low Impact Docking System (LIDS). The acronym international Low Impact Docking System (iLIDS) is also used to describe this system as well as the Government Furnished Equipment (GFE) project designing the NDS for the NDSP. NDS and iLIDS may be used interchangeability. This document will use the acronym iLIDS. Some of the heritage documentation and implementations (e.g., software command names, requirement identification (ID), figures, etc.) used on NDS will continue to use the LIDS acronym. This specification defines the technical requirements for the iLIDS GFE delivered to the NDSP by the iLIDS project. This document contains requirements for two iLIDS configurations, SEZ29101800-301 and SEZ29101800-302. Requirements with the statement, iLIDS shall, are for all configurations. Examples of requirements that are unique to a single configuration may be identified as iLIDS (-301) shall or iLIDS (-302) shall. Furthermore, to allow a requirement to encompass all configurations with an exception, the requirement may be designated as iLIDS (excluding -302) shall. Verification requirements for the iLIDS project are identified in the Verification Matrix (VM) provided in the iLIDS Verification and Validation Document, JSC-63966. The following definitions differentiate between requirements and other statements: Shall: This is the only verb used for the binding requirements. Should/May: These verbs are used for stating non-mandatory goals. Will: This verb is used for stating facts or declaration of purpose. A Definition of Terms table is provided in Appendix B to define those terms with specific tailored uses in this document.

  8. NAIP proteins are required for cytosolic detection of specific bacterial ligands in vivo.

    PubMed

    Rauch, Isabella; Tenthorey, Jeannette L; Nichols, Randilea D; Al Moussawi, Khatoun; Kang, James J; Kang, Chulho; Kazmierczak, Barbara I; Vance, Russell E

    2016-05-01

    NLRs (nucleotide-binding domain [NBD] leucine-rich repeat [LRR]-containing proteins) exhibit diverse functions in innate and adaptive immunity. NAIPs (NLR family, apoptosis inhibitory proteins) are NLRs that appear to function as cytosolic immunoreceptors for specific bacterial proteins, including flagellin and the inner rod and needle proteins of bacterial type III secretion systems (T3SSs). Despite strong biochemical evidence implicating NAIPs in specific detection of bacterial ligands, genetic evidence has been lacking. Here we report the use of CRISPR/Cas9 to generate Naip1(-/-) and Naip2(-/-) mice, as well as Naip1-6(Δ/Δ) mice lacking all functional Naip genes. By challenging Naip1(-/-) or Naip2(-/-) mice with specific bacterial ligands in vivo, we demonstrate that Naip1 is uniquely required to detect T3SS needle protein and Naip2 is uniquely required to detect T3SS inner rod protein, but neither Naip1 nor Naip2 is required for detection of flagellin. Previously generated Naip5(-/-) mice retain some residual responsiveness to flagellin in vivo, whereas Naip1-6(Δ/Δ) mice fail to respond to cytosolic flagellin, consistent with previous biochemical data implicating NAIP6 in flagellin detection. Our results provide genetic evidence that specific NAIP proteins function to detect specific bacterial proteins in vivo. PMID:27045008

  9. SRS BEDROCK PROBABILISTIC SEISMIC HAZARD ANALYSIS (PSHA) DESIGN BASIS JUSTIFICATION (U)

    SciTech Connect

    , R

    2005-12-14

    This represents an assessment of the available Savannah River Site (SRS) hard-rock probabilistic seismic hazard assessments (PSHAs), including PSHAs recently completed, for incorporation in the SRS seismic hazard update. The prior assessment of the SRS seismic design basis (WSRC, 1997) incorporated the results from two PSHAs that were published in 1988 and 1993. Because of the vintage of these studies, an assessment is necessary to establish the value of these PSHAs considering more recently collected data affecting seismic hazards and the availability of more recent PSHAs. This task is consistent with the Department of Energy (DOE) order, DOE O 420.1B and DOE guidance document DOE G 420.1-2. Following DOE guidance, the National Map Hazard was reviewed and incorporated in this assessment. In addition to the National Map hazard, alternative ground motion attenuation models (GMAMs) are used with the National Map source model to produce alternate hazard assessments for the SRS. These hazard assessments are the basis for the updated hard-rock hazard recommendation made in this report. The development and comparison of hazard based on the National Map models and PSHAs completed using alternate GMAMs provides increased confidence in this hazard recommendation. The alternate GMAMs are the EPRI (2004), USGS (2002) and a regional specific model (Silva et al., 2004). Weights of 0.6, 0.3 and 0.1 are recommended for EPRI (2004), USGS (2002) and Silva et al. (2004) respectively. This weighting gives cluster weights of .39, .29, .15, .17 for the 1-corner, 2-corner, hybrid, and Greens-function models, respectively. This assessment is judged to be conservative as compared to WSRC (1997) and incorporates the range of prevailing expert opinion pertinent to the development of seismic hazard at the SRS. The corresponding SRS hard-rock uniform hazard spectra are greater than the design spectra developed in WSRC (1997) that were based on the LLNL (1993) and EPRI (1988) PSHAs. The

  10. Towards the formal specification of the requirements and design of a processor interface unit

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1993-01-01

    Work to formally specify the requirements and design of a Processor Interface Unit (PIU), a single-chip subsystem providing memory interface, bus interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system, is described. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance free operation, or both. The approaches that were developed for modeling the PIU requirements and for composition of the PIU subcomponents at high levels of abstraction are described. These approaches were used to specify and verify a nontrivial subset of the PIU behavior. The PIU specification in Higher Order Logic (HOL) is documented in a companion NASA contractor report entitled 'Towards the Formal Specification of the Requirements and Design of a Processor Interfacs Unit - HOL Listings.' The subsequent verification approach and HOL listings are documented in NASA contractor report entitled 'Towards the Formal Verification of the Requirements and Design of a Processor Interface Unit' and NASA contractor report entitled 'Towards the Formal Verification of the Requirements and Design of a Processor Interface Unit - HOL Listings.'

  11. NASA specification for manufacturing and performance requirements of NASA standard aerospace nickel-cadmium cells

    NASA Technical Reports Server (NTRS)

    1988-01-01

    On November 25, 1985, the NASA Chief Engineer established a NASA-wide policy to maintain and to require the use of the NASA standard for aerospace nickel-cadmium cells and batteries. The Associate Administrator for Safety, Reliability, Maintainability, and Quality Assurance stated on December 29, 1986, the intent to retain the NASA standard cell usage policy established by the Office of the Chief Engineer. The current NASA policy is also to incorporate technological advances as they are tested and proven for spaceflight applications. This policy will be implemented by modifying the existing standard cells or by developing new NASA standards and their specifications in accordance with the NASA's Aerospace Battery Systems Program Plan. This NASA Specification for Manufacturing and Performance Requirements of NASA Standard Aerospace Nickel-Cadmium Cells is prepared to provide requirements for the NASA standard nickel-cadmium cell. It is an interim specification pending resolution of the separator material availability. This specification has evolved from over 15 years of nickel-cadmium cell experience by NASA. Consequently, considerable experience has been collected and cell performance has been well characterized from many years of ground testing and from in-flight operations in both geosynchronous (GEO) and low earth orbit (LEO) applications. NASA has developed and successfully used two standard flight qualified cell designs.

  12. A discussion of higher order software concepts as they apply to functional requirements and specifications. [space shuttles and guidance

    NASA Technical Reports Server (NTRS)

    Hamilton, M.

    1973-01-01

    The entry guidance software functional requirements (requirements design phase), its architectural requirements (specifications design phase), and the entry guidance software verified code are discussed. It was found that the proper integration of designs at both the requirements and specifications levels are of high priority consideration.

  13. Shedding new light on lipid functions with CARS and SRS microscopy

    PubMed Central

    Yu, Yong; Ramachandran, Prasanna V.; Wang, Meng C.

    2014-01-01

    Modern optical microscopy has granted biomedical scientists unprecedented access to the inner workings of a cell, and revolutionized our understanding of the molecular mechanisms underlying physiological and disease states. In spite of these advances, however, visualization of certain classes of molecules (e.g. lipids) at the sub-cellular level has remained elusive. Recently developed chemical imaging modalities – Coherent Anti-Stokes Raman Scattering (CARS) microscopy and Stimulated Raman Scattering (SRS) microscopy – have helped bridge this gap. By selectively imaging the vibration of a specific chemical group, these non-invasive techniques allow high-resolution imaging of individual molecules in vivo, and circumvent the need for potentially perturbative extrinsic labels. These tools have already been applied to the study of fat metabolism, helping uncover novel regulators of lipid storage. Here we review the underlying principle of CARS and SRS microscopy, and discuss the advantages and caveats of each technique. We also review recent applications of these tools in the study of lipids as well as other biomolecules, and conclude with a brief guide for interested researchers to build and use CARS/SRS systems for their own research. PMID:24576891

  14. Relaxation of rod misalignment technical specification requirements: An increased operational flexibility

    SciTech Connect

    John, G.; Leipner-Gomes, C.I.; Johansen, B.J.

    1995-12-31

    The pressurized water reactor technical specification (T/S) limiting condition for operation (LCO) for rod misalignment and rod position indication (RPI) typically requires all rod cluster control assemblies to be positioned with {+-}12 steps of the group demand position. The LCO is approved by the U.S. Nuclear Regulatory Commission (NRC) for use by all Westinghouse plants, and the core design incorporates a peaking factor uncertainty to accommodate potential rod misalignments within this approved LCO. Also, the LCO requires all RPI channels and the demand position indicator system to be operable and capable of determining the control rod position within {+-}12 steps.

  15. Assessment of SRS radiological liquid and airborne contaminants and pathways

    SciTech Connect

    Jannik, G.T.

    1997-04-01

    This report compiles and documents the radiological critical-contaminant/critical-pathway analysis performed for SRS. The analysis covers radiological releases to the atmosphere and to surface water, which are the principal media that carry contaminants off site. During routine operations at SRS, limited amounts of radionuclides are released to the environment through atmospheric and/or liquid pathways. These releases potentially result in exposure to offsite people. Though the groundwater beneath an estimated 5 to 10 percent of SRS has been contaminated by radionuclides, there is no evidence that groundwater contaminated with these constituents has migrated offsite (Arnett, 1996). Therefore, with the notable exception of radiological source terms originating from shallow surface water migration into site streams, onsite groundwater was not considered as a potential exposure pathway to offsite people.

  16. Gamma Ray Observatory (GRO) dynamics simulator requirements and mathematical specifications, revision 1

    NASA Technical Reports Server (NTRS)

    Harman, R.; Blejer, D.

    1990-01-01

    The requirements and mathematical specifications for the Gamma Ray Observatory (GRO) Dynamics Simulator are presented. The complete simulator system, which consists of the profie subsystem, simulation control and input/output subsystem, truth model subsystem, onboard computer model subsystem, and postprocessor, is described. The simulator will be used to evaluate and test the attitude determination and control models to be used on board GRO under conditions that simulate the expected in-flight environment.

  17. Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid*

    PubMed Central

    Patel, Avnish; Mohl, Bjorn-Patrick; Roy, Polly

    2016-01-01

    The entry of viruses into host cells is one of the key processes of infection. The mechanisms of cellular entry for enveloped virus have been well studied. The fusion proteins as well as the facilitating cellular lipid factors involved in the viral fusion entry process have been well characterized. The process of non-enveloped virus cell entry, in comparison, remains poorly defined, particularly for large complex capsid viruses of the family Reoviridae, which comprises a range of mammalian pathogens. These viruses enter cells without the aid of a limiting membrane and thus cannot fuse with host cell membranes to enter cells. Instead, these viruses are believed to penetrate membranes of the host cell during endocytosis. However, the molecular mechanism of this process is largely undefined. Here we show, utilizing an in vitro liposome penetration assay and cell biology, that bluetongue virus (BTV), an archetypal member of the Reoviridae, utilizes the late endosome-specific lipid lysobisphosphatidic acid for productive membrane penetration and viral entry. Further, we provide preliminary evidence that lipid lysobisphosphatidic acid facilitates pore expansion during membrane penetration, suggesting a mechanism for lipid factor requirement of BTV. This finding indicates that despite the lack of a membrane envelope, the entry process of BTV is similar in specific lipid requirements to enveloped viruses that enter cells through the late endosome. These results are the first, to our knowledge, to demonstrate that a large non-enveloped virus of the Reoviridae has specific lipid requirements for membrane penetration and host cell entry. PMID:27036941

  18. Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation.

    PubMed

    Baker, Charli D; Basu Ball, Writoban; Pryce, Erin N; Gohil, Vishal M

    2016-07-15

    Mitochondrial membrane phospholipid composition affects mitochondrial function by influencing the assembly of the mitochondrial respiratory chain (MRC) complexes into supercomplexes. For example, the loss of cardiolipin (CL), a signature non-bilayer-forming phospholipid of mitochondria, results in disruption of MRC supercomplexes. However, the functions of the most abundant mitochondrial phospholipids, bilayer-forming phosphatidylcholine (PC) and non-bilayer-forming phosphatidylethanolamine (PE), are not clearly defined. Using yeast mutants of PE and PC biosynthetic pathways, we show a specific requirement for mitochondrial PE in MRC complex III and IV activities but not for their formation, whereas loss of PC does not affect MRC function or formation. Unlike CL, mitochondrial PE or PC is not required for MRC supercomplex formation, emphasizing the specific requirement of CL in supercomplex assembly. Of interest, PE biosynthesized in the endoplasmic reticulum (ER) can functionally substitute for the lack of mitochondrial PE biosynthesis, suggesting the existence of PE transport pathway from ER to mitochondria. To understand the mechanism of PE transport, we disrupted ER-mitochondrial contact sites formed by the ERMES complex and found that, although not essential for PE transport, ERMES facilitates the efficient rescue of mitochondrial PE deficiency. Our work highlights specific roles of non-bilayer-forming phospholipids in MRC function and formation. PMID:27226479

  19. Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid.

    PubMed

    Patel, Avnish; Mohl, Bjorn-Patrick; Roy, Polly

    2016-06-01

    The entry of viruses into host cells is one of the key processes of infection. The mechanisms of cellular entry for enveloped virus have been well studied. The fusion proteins as well as the facilitating cellular lipid factors involved in the viral fusion entry process have been well characterized. The process of non-enveloped virus cell entry, in comparison, remains poorly defined, particularly for large complex capsid viruses of the family Reoviridae, which comprises a range of mammalian pathogens. These viruses enter cells without the aid of a limiting membrane and thus cannot fuse with host cell membranes to enter cells. Instead, these viruses are believed to penetrate membranes of the host cell during endocytosis. However, the molecular mechanism of this process is largely undefined. Here we show, utilizing an in vitro liposome penetration assay and cell biology, that bluetongue virus (BTV), an archetypal member of the Reoviridae, utilizes the late endosome-specific lipid lysobisphosphatidic acid for productive membrane penetration and viral entry. Further, we provide preliminary evidence that lipid lysobisphosphatidic acid facilitates pore expansion during membrane penetration, suggesting a mechanism for lipid factor requirement of BTV. This finding indicates that despite the lack of a membrane envelope, the entry process of BTV is similar in specific lipid requirements to enveloped viruses that enter cells through the late endosome. These results are the first, to our knowledge, to demonstrate that a large non-enveloped virus of the Reoviridae has specific lipid requirements for membrane penetration and host cell entry. PMID:27036941

  20. Savannah River Site (SRS) implementation program plan for DNFSB Recommendation 90-2

    SciTech Connect

    Talukdar, B.K.; Loceff, F.

    1993-10-01

    The Defense Nuclear Facilities Safety Board (DNFSB) based on its review and evaluation of the content and implementation of standards relating to design, construction, operation, and decommissioning of Defense Nuclear Facilities has made the recommendations (90-2) which when implemented would assure comparable or equivalent levels of safety to the environment, public and workers as required for the commercial nuclear facilities. DOE has accepted the DNFSB 90-2 recommendations and have directed SRS and other M&Os to implement them. This report discusses implementation program which commits to developing Requirement Identification Documents (RID`s) for all defense nuclear facilities in the DOE complex.

  1. Glia-derived neurons are required for sex-specific learning in C. elegans

    PubMed Central

    Sammut, Michele; Cook, Steven J.; Nguyen, Ken C.Q.; Felton, Terry; Hall, David H.; Emmons, Scott W.

    2015-01-01

    Sex differences in behaviour extend to cognitive-like processes such as learning but the underlying dimorphisms in neural circuit development and organization that generate these behavioural differences are largely unknown. Here we define at the single-cell level, from development, through neural circuit connectivity, to function, the neural basis of a sex-specific learning in the nematode C. elegans. We show that sexual conditioning, a form of associative learning, requires a pair of male-specific interneurons whose progenitors are fully differentiated glia. These neurons are born during sexual maturation and incorporated into pre-exisiting sex-shared circuits to couple chemotactic responses to reproductive priorities. Our findings reveal a general role for glia as neural progenitors across metazoan taxa and demonstrate that the addition of sex-specific neuron types to brain circuits during sexual maturation is an important mechanism for the generation of sexually dimorphic plasticity in learning. PMID:26469050

  2. Glia-derived neurons are required for sex-specific learning in C. elegans.

    PubMed

    Sammut, Michele; Cook, Steven J; Nguyen, Ken C Q; Felton, Terry; Hall, David H; Emmons, Scott W; Poole, Richard J; Barrios, Arantza

    2015-10-15

    Sex differences in behaviour extend to cognitive-like processes such as learning, but the underlying dimorphisms in neural circuit development and organization that generate these behavioural differences are largely unknown. Here we define at the single-cell level-from development, through neural circuit connectivity, to function-the neural basis of a sex-specific learning in the nematode Caenorhabditis elegans. We show that sexual conditioning, a form of associative learning, requires a pair of male-specific interneurons whose progenitors are fully differentiated glia. These neurons are generated during sexual maturation and incorporated into pre-exisiting sex-shared circuits to couple chemotactic responses to reproductive priorities. Our findings reveal a general role for glia as neural progenitors across metazoan taxa and demonstrate that the addition of sex-specific neuron types to brain circuits during sexual maturation is an important mechanism for the generation of sexually dimorphic plasticity in learning. PMID:26469050

  3. AMTD: Update of Engineering Specifications Derived from Science Requirements for Future UVOIR Space Telescopes

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip; Postman, Marc; Mosier, Gary; Smith, W. Scott; Blaurock, Carl; Ha, Kong; Stark, Christopher C.

    2014-01-01

    The Advance Mirror Technology Development (AMTD) project is in Phase 2 of a multiyear effort, initiated in FY12, to mature by at least a half TRL step six critical technologies required to enable 4 meter or larger UVOIR space telescope primary mirror assemblies for both general astrophysics and ultra-high contrast observations of exoplanets. AMTD uses a science-driven systems engineering approach. We mature technologies required to enable the highest priority science AND provide a high-performance low-cost low-risk system. To give the science community options, we are pursuing multiple technology paths. A key task is deriving engineering specifications for advanced normal-incidence monolithic and segmented mirror systems needed to enable both general astrophysics and ultra-high contrast observations of exoplanets missions as a function of potential launch vehicles and their mass and volume constraints. A key finding of this effort is that the science requires an 8 meter or larger aperture telescope

  4. AMTD: update of engineering specifications derived from science requirements for future UVOIR space telescopes

    NASA Astrophysics Data System (ADS)

    Stahl, H. Philip; Postman, Marc; Mosier, Gary; Smith, W. Scott; Blaurock, Carl; Ha, Kong; Stark, Christopher C.

    2014-08-01

    The Advance Mirror Technology Development (AMTD) project is in Phase 2 of a multiyear effort, initiated in FY12, to mature by at least a half TRL step six critical technologies required to enable 4 meter or larger UVOIR space telescope primary mirror assemblies for both general astrophysics and ultra-high contrast observations of exoplanets. AMTD uses a science-driven systems engineering approach. We mature technologies required to enable the highest priority science AND provide a high-performance low-cost low-risk system. To give the science community options, we are pursuing multiple technology paths. A key task is deriving engineering specifications for advanced normal-incidence monolithic and segmented mirror systems needed to enable both general astrophysics and ultra-high contrast observations of exoplanets missions as a function of potential launch vehicles and their mass and volume constraints. A key finding of this effort is that the science requires an 8 meter or larger aperture telescope.

  5. Body size and human energy requirements: Reduced mass-specific total energy expenditure in tall adults.

    PubMed

    Heymsfield, Steven B; Pietrobelli, Angelo

    2010-01-01

    Mammalian resting energy expenditure (REE) increases as approximately weight(0.75) while mass-specific REE scales as approximately weight(-0.25). Energy needs for replacing resting losses are thus less relative to weight (W) in large compared with small mammals, a classic observation with biological implications. Human weight scales as approximately height(2) and tall adults thus have a greater weight than their short counterparts. However, it remains unknown if mass-specific energy requirements are less in tall adults; allometric models linking total energy expenditure (TEE) and weight with height (H) are lacking. We tested the hypothesis that mass-specific energy requirements scale inversely to height in adults by evaluating TEE (doubly labeled water) data collected by the National Academy of Sciences. Activity energy expenditure (AEE) was calculated from TEE, REE (indirect calorimetry), and estimated diet-induced energy expenditure. Main analyses focused on nonmorbidly obese subjects < or =50 yrs of age with non-negative AEE values (n = 404), although results were directionally similar for all samples. Allometric models, including age as a covariate, revealed significantly (P < 0.05) greater REE, AEE, and TEE as a function of height (range H(1.5-1.7)) in both men and women. TEE/W scaled negatively to height ( approximately H(-0.7), P < 0.01) with predicted mass-specific TEE (kcal/kg/d) at +/-2 SD for US height lower in tall compared with short men (40.3 vs. 46.5) and women (37.7 vs. 42.7). REE/W also scaled negatively to height in men (P < 0.001) and women (P < 0.01). Results were generally robust across several different analytic strategies. These observations reveal previously unforeseen associations between human stature and energy requirements that have implications for modeling efforts and provide new links to mammalian biology as a whole. PMID:19856424

  6. Screening Leishmania donovani complex-specific genes required for visceral disease.

    PubMed

    Zhang, Wen-Wei; Matlashewski, Greg

    2015-01-01

    Leishmania protozoan parasites are the causing agent of leishmaniasis. Depending on the infecting species, Leishmania infection can causes a wide variety of diseases such as self-healing cutaneous lesions by L. major and fatal visceral leishmaniasis by L. donovani and L. infantum. Comparison of the visceral disease causing L. infantum genome with cutaneous disease causing L. major and L. braziliensis genomes has identified 25 L. infantum (L. donovani complex) species-specific genes that are absent or pseudogenes in L. major and L. braziliensis. To investigate whether these L. donovani complex species-specific genes are involved in visceral infection, we cloned these genes from L. donovani and introduced them into L. major and then determined whether the transgenic L. major had an increased ability to survive in liver and spleen of BALB/c mice. Several of these L. donovani complex specific genes were found to significantly increase L. major survival in visceral organs in BALB/c mice including the A2 and Ld2834 genes, while down regulation of these genes in L. donovani by either antisense RNA or gene knockout dramatically reduced L. donovani virulence in BALB/c mice. This demonstrated that L. donovani complex species-specific genes play important roles in visceral infection. In this chapter, we describe procedures to screen L. donovani complex specific genes required for visceral infection by cross species transgenic expression, gene deletion targeting and measuring infection levels in mice. PMID:25388124

  7. Extraembryonic but not embryonic SUMO-specific protease 2 is required for heart development

    PubMed Central

    Maruyama, Eri O.; Lin, Heng; Chiu, Shang-Yi; Yu, H.-M. Ivy; Porter, George A.; Hsu, Wei

    2016-01-01

    SUMO-specific protease 2 (SENP2) activities to remove SUMO from its substrates is essential for development of trophoblast stem cells, niches and lineages. Global deletion of SENP2 leads to midgestation lethality, and causes severe defects in the placenta which is accompanied by embryonic brain and heart abnormalities. Because of the placental deficiencies, the role of SENP2 in development of the embryonic tissues has not been properly determined. The brain and heart abnormalities may be secondary to placental insufficiency. Here we have created a new mouse strain permitting conditional inactivation of SENP2. Mice homozygous for germline deletion of the conditional allele exhibit trophoblast defects and embryonic abnormalities resembling the global SENP2 knockout. However, tissue-specific disruptions of SENP2 demonstrate its dispensable role in embryogenesis. Placental expression of SENP2 is necessary and sufficient for embryonic heart and brain development. Using a protease deficient model, we further demonstrate the requirement of SENP2-dependent SUMO modification in development of all major trophoblast lineages. SENP2 regulates sumoylation of Mdm2 which controls p53 activities critical for G-S transition of mitotic division and endoreduplication in trophoblast proliferation and differentiation, respectively. The differentiation of trophoblasts is also dependent on SENP2-mediated activation of p57Kip2, a CDK-specific inhibitor required for endoreduplication. PMID:26883797

  8. Temporal and Region-Specific Requirements of αCaMKII in Spatial and Contextual Learning

    PubMed Central

    Achterberg, Katharina G.; Buitendijk, Gabriëlle H.S.; Kool, Martijn J.; Goorden, Susanna M.I.; Post, Laura; Slump, Denise E.; Silva, Alcino J.; van Woerden, Geeske M.

    2014-01-01

    The α isoform of the calcium/calmodulin-dependent protein kinase II (αCaMKII) has been implicated extensively in molecular and cellular mechanisms underlying spatial and contextual learning in a wide variety of species. Germline deletion of Camk2a leads to severe deficits in spatial and contextual learning in mice. However, the temporal and region-specific requirements for αCaMKII have remained largely unexplored. Here, we generated conditional Camk2a mutants to examine the influence of spatially restricted and temporally controlled expression of αCaMKII. Forebrain-specific deletion of the Camk2a gene resulted in severe deficits in water maze and contextual fear learning, whereas mice with deletion restricted to the cerebellum learned normally. Furthermore, we found that temporally controlled deletion of the Camk2a gene in adult mice is as detrimental as germline deletion for learning and synaptic plasticity. Together, we confirm the requirement for αCaMKII in the forebrain, but not the cerebellum, in spatial and contextual learning. Moreover, we highlight the absolute requirement for intact αCaMKII expression at the time of learning. PMID:25143599

  9. SRS Software Verification Pre Operational and Startup Test

    SciTech Connect

    HILL, L.F.

    2000-10-18

    This document defines testing for the software used to control the Sodium Removal System (SRS). The testing is conducted off-line from the. physical plant by using a simulator built-in to the software. This provides verification of proper software operation prior to performing the operational acceptance tests with the actual plant hardware.

  10. Wastewaters at SRS where heavy metals are a potential problem

    SciTech Connect

    Wilde, E.W.; Radway, J.C.

    1994-11-01

    The principal objective of this report is to identify and prioritize heavy metal-containing wastewaters at the Savannah River Site (SRS) in terms of their suitability for testing of and clean-up by a novel bioremediation process being developed by SRTC. This process involves the use of algal biomass for sequestering heavy metal and radionuclides from wastewaters. Two categories of SRS wastewaters were considered for this investigation: (1) waste sites (primarily non-contained wastes managed by Environmental Restoration), and (2) waste streams (primarily contained wastes managed by Waste Management). An attempt was made to evaluate all sources of both categories of waste throughout the site so that rational decisions could be made with regard to selecting the most appropriate wastewaters for present study and potential future treatment. The investigation included a review of information on surface and/or groundwater associated with all known SRS waste sites, as well as waters associated with all known SRS waste streams. Following the initial review, wastewaters known or suspected to contain potentially problematic concentrations of one or more of the toxic metals were given further consideration.

  11. Risk-Dominant Scenarios from Several SRS Facilities

    SciTech Connect

    Blanchard, A.

    1999-03-15

    This report and associated spreadsheets describe the SRS safety analysis provided for four selected transuranic storage and stabilization facilities. For each of the four sets of analysis, the bounding events in each frequency category are identified, key inputs and assumptions are stated, and final doses tabulated.

  12. DNA sequence-specific recognition by a transcriptional regulator requires indirect readout of A-tracts

    PubMed Central

    Mendieta, Jesús; Pérez-Lago, Laura; Salas, Margarita; Camacho, Ana

    2007-01-01

    The bacteriophage Ø29 transcriptional regulator p4 binds to promoters of different intrinsic activities. The p4–DNA complex contains two identical protomers that make similar interactions with the target sequence 5′-AACTTTTT-15 bp-AAAATGTT-3′. To define how the various elements in the target sequence contribute to p4's affinity, we studied p4 binding to a series of mutated binding sites. The binding specificity depends critically on base pairs of the target sequence through both direct as well as indirect readout. There is only one specific contact between a base and an amino acid residue; other contacts take place with the phosphate backbone. Alteration of direct amino acid–base contacts, or mutation of non-contacted A·T base pairs at A-tracts abolished binding. We generated three 5 ns molecular dynamics (MD) simulations to investigate the basis for the p4–DNA complex specificity. Recognition is controlled by the protein and depends on DNA dynamic properties. MD results on protein–DNA contacts and the divergence of p4 affinity to modified binding sites reveal an inherent asymmetry, which is required for p4-specific binding and may be crucial for transcription regulation. PMID:17452358

  13. Specifications of the octupole magnets required for the ATF2 ultra-low ß* lattice

    SciTech Connect

    Marin, E.; Modena, M.; Tauchi, T.; Terunuma, N.; Tomas, R.; White, G.R.; /SLAC

    2014-05-28

    The Accelerator Test Facility 2 (ATF2) aims to test the novel chromaticity correction for higher chromaticity lattices as the one of CLIC. To this end the ATF2 ultra-low ß* lattice is designed to vertically focus the beam at the focal point or usually referred to as interaction point (IP), down to 23 nm. However when the measured multipole components of the ATF2 magnets are considered in the simulations, the evaluated spot sizes at the IP are well above the design value. The designed spot size is effectively recovered by inserting a pair of octupole magnets. In this note we address the technical specifications required for these octupole magnets.

  14. STATS SRS v11.0

    SciTech Connect

    Piscotty, M A; Nazario, O L

    2007-06-20

    The objective of this project is the delivery of an application that will provide a unified, web-based system for collecting, verifying and analyzing the achievements for Laboratory employees. The application will enable individual Directorates to manage and report achievement record data for their employees using an LLNL standard web browser. In addition, cross directorate data reporting and analysis will be available for such organizations as LSTO and programmatic directorates. This system is intended to store reference data and metadata for employee achievements. Abstracts and entire publications will not be stored in this system.Directorates are expected to use this system at all levels of management in preparing for Annual Self-Assessments, peer reviews, LDRD reviews, work force reviews, performance appraisals, and requests from sponsors. This document represents the primary deliverable for the Requirements Definition stage of system development. As part of a successful Requirements Definition, this document provides the development staff, the project sponsor, and the user community with a clear understanding of the product's operational, data, and other requirements. With this understanding, the development staff will take the opportunity to refine estimates regarding the cost, schedule, and deliverables reflected in it.

  15. 10 CFR 33.15 - Requirements for the issuance of a Type C specific license of broad scope.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Requirements for the issuance of a Type C specific license of broad scope. 33.15 Section 33.15 Energy NUCLEAR REGULATORY COMMISSION SPECIFIC DOMESTIC LICENSES OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.15 Requirements for the issuance of a Type C specific license of...

  16. 10 CFR 33.13 - Requirements for the issuance of a Type A specific license of broad scope.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Requirements for the issuance of a Type A specific license of broad scope. 33.13 Section 33.13 Energy NUCLEAR REGULATORY COMMISSION SPECIFIC DOMESTIC LICENSES OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.13 Requirements for the issuance of a Type A specific license of...

  17. 10 CFR 33.13 - Requirements for the issuance of a Type A specific license of broad scope.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Requirements for the issuance of a Type A specific license of broad scope. 33.13 Section 33.13 Energy NUCLEAR REGULATORY COMMISSION SPECIFIC DOMESTIC LICENSES OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.13 Requirements for the issuance of a Type A specific license of...

  18. 10 CFR 33.13 - Requirements for the issuance of a Type A specific license of broad scope.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Requirements for the issuance of a Type A specific license of broad scope. 33.13 Section 33.13 Energy NUCLEAR REGULATORY COMMISSION SPECIFIC DOMESTIC LICENSES OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.13 Requirements for the issuance of a Type A specific license of...

  19. The Efficacy of Pneumococcal Capsular Polysaccharide-specific Antibodies to Serotype 3 Streptococcus pneumoniae requires Macrophages

    PubMed Central

    Fabrizio, Kevin; Manix, Catherine; Tian, Haijun; van Rooijen, Nico; Pirofski, Liise-anne

    2010-01-01

    The efficacy of antibody immunity against Streptococcus pneumoniae stems from the ability of opsonic, serotype (ST)-specific antibodies to pneumococcal capsular polysaccharide (PPS) to facilitate killing of the homologous ST by host phagocytes. However, PPS-specific antibodies have been identified that are protective in mice, but do not promote opsonic killing in vitro, raising the question of how they mediate protection in vivo. To probe this question, we investigated the dependence of antibody efficacy against lethal systemic (intraperitoneal, i.p.) infection with Streptococcus pneumoniae serotype 3 (ST3) on macrophages and neutrophils for the following PPS3-specific monoclonal antibodies (MAbs) in survival experiments in mice using a non-opsonic human IgM (A7), a non-opsonic mouse IgG1 (1E2) and an opsonic mouse IgG1 (5F6). The survival of A7- and PPS3-specific and isotype control-MAb-treated neutrophil-depleted and neutrophil-sufficient and macrophage-depleted and macrophage-sufficient mice were determined after i.p. challenge with ST3 strains 6303 and WU2. Neutrophils were dispensable for A7 and the mouse MAbs to mediate protection in this model, but macrophages were required for the efficacy of A7 and optimal mouse MAb-mediated protection. For A7-treated mice, macrophage-depleted mice had higher blood CFU, cytokines and peripheral neutrophil levels than macrophage-sufficient mice, and macrophage-sufficient mice had lower tissue bacterial burdens than control MAb-treated mice. These findings demonstrate that macrophages contribute to opsonic and non-opsonic PPS3-specific MAb-mediated protection against ST3 infection by enhancing bacterial clearance and suggest that neutrophils do not compensate for the absence of macrophages in the model used in this study. PMID:20800700

  20. Fgf16 Is Required for Specification of GABAergic Neurons and Oligodendrocytes in the Zebrafish Forebrain

    PubMed Central

    Miyake, Ayumi; Chitose, Tatsuya; Kamei, Eriko; Murakami, Atsuko; Nakayama, Yoshiaki; Konishi, Morichika; Itoh, Nobuyuki

    2014-01-01

    Fibroblast growth factor (Fgf) signaling plays crucial roles in various developmental processes including those in the brain. We examined the role of Fgf16 in the formation of the zebrafish brain. The knockdown of fgf16 decreased cell proliferation in the forebrain and midbrain. fgf16 was also essential for development of the ventral telencephalon and diencephalon, whereas fgf16 was not required for dorsoventral patterning in the midbrain. fgf16 was additionally required for the specification and differentiation of γ–aminobutyric acid (GABA)ergic interneurons and oligodendrocytes, but not for those of glutamatergic neurons in the forebrain. Cross talk between Fgf and Hedgehog (Hh) signaling was critical for the specification of GABAergic interneurons and oligodendrocytes. The expression of fgf16 in the forebrain was down-regulated by the inhibition of Hh and Fgf19 signaling, but not by that of Fgf3/Fgf8 signaling. The fgf16 morphant phenotype was similar to that of the fgf19 morphant and embryos blocked Hh signaling. The results of the present study indicate that Fgf16 signaling, which is regulated by the downstream pathways of Hh-Fgf19 in the forebrain, is involved in forebrain development. PMID:25357195

  1. Hmga2 is required for neural crest cell specification in Xenopus laevis.

    PubMed

    Macrì, Simone; Simula, Luca; Pellarin, Ilenia; Pegoraro, Silvia; Onorati, Marco; Sgarra, Riccardo; Manfioletti, Guidalberto; Vignali, Robert

    2016-03-01

    HMGA proteins are small nuclear proteins that bind DNA by conserved AT-hook motifs, modify chromatin architecture and assist in gene expression. Two HMGAs (HMGA1 and HMGA2), encoded by distinct genes, exist in mammals and are highly expressed during embryogenesis or reactivated in tumour progression. We here addressed the in vivo role of Xenopus hmga2 in the neural crest cells (NCCs). We show that hmga2 is required for normal NCC specification and development. hmga2 knockdown leads to severe disruption of major skeletal derivatives of anterior NCCs. We show that, within the NCC genetic network, hmga2 acts downstream of msx1, and is required for msx1, pax3 and snail2 activities, thus participating at different levels of the network. Because of hmga2 early effects in NCC specification, the subsequent epithelial-mesenchymal transition (EMT) and migration of NCCs towards the branchial pouches are also compromised. Strictly paralleling results on embryos, interfering with Hmga2 in a breast cancer cell model for EMT leads to molecular effects largely consistent with those observed on NCCs. These data indicate that Hmga2 is recruited in key molecular events that are shared by both NCCs and tumour cells. PMID:26806704

  2. Neurotransmitter Transporter-Like: A Male Germline-specific SLC6 Transporter Required for Drosophila Spermiogenesis

    PubMed Central

    Chatterjee, Nabanita; Rollins, Janet; Mahowald, Anthony P.; Bazinet, Christopher

    2011-01-01

    The SLC6 class of membrane transporters, known primarily as neurotransmitter transporters, is increasingly appreciated for its roles in nutritional uptake of amino acids and other developmentally specific functions. A Drosophila SLC6 gene, Neurotransmitter transporter-like (Ntl), is expressed only in the male germline. Mobilization of a transposon inserted near the 3′ end of the Ntl coding region yields male-sterile mutants defining a single complementation group. Germline transformation with Ntl cDNAs under control of male germline-specific control elements restores Ntl/Ntl homozygotes to normal fertility, indicating that Ntl is required only in the germ cells. In mutant males, sperm morphogenesis appears normal, with elongated, individualized and coiled spermiogenic cysts accumulating at the base of the testes. However, no sperm are transferred to the seminal vesicle. The level of polyglycylation of Ntl mutant sperm tubulin appears to be significantly lower than that of wild type controls. Glycine transporters are the most closely related SLC6 transporters to Ntl, suggesting that Ntl functions as a glycine transporter in developing sperm, where augmentation of the cytosolic pool of glycine may be required for the polyglycylation of the massive amounts of tubulin in the fly's giant sperm. The male-sterile phenotype of Ntl mutants may provide a powerful genetic system for studying the function of an SLC6 transporter family in a model organism. PMID:21298005

  3. An Automated Method for Identifying Inconsistencies within Diagrammatic Software Requirements Specifications

    NASA Technical Reports Server (NTRS)

    Zhang, Zhong

    1997-01-01

    The development of large-scale, composite software in a geographically distributed environment is an evolutionary process. Often, in such evolving systems, striving for consistency is complicated by many factors, because development participants have various locations, skills, responsibilities, roles, opinions, languages, terminology and different degrees of abstraction they employ. This naturally leads to many partial specifications or viewpoints. These multiple views on the system being developed usually overlap. From another aspect, these multiple views give rise to the potential for inconsistency. Existing CASE tools do not efficiently manage inconsistencies in distributed development environment for a large-scale project. Based on the ViewPoints framework the WHERE (Web-Based Hypertext Environment for requirements Evolution) toolkit aims to tackle inconsistency management issues within geographically distributed software development projects. Consequently, WHERE project helps make more robust software and support software assurance process. The long term goal of WHERE tools aims to the inconsistency analysis and management in requirements specifications. A framework based on Graph Grammar theory and TCMJAVA toolkit is proposed to detect inconsistencies among viewpoints. This systematic approach uses three basic operations (UNION, DIFFERENCE, INTERSECTION) to study the static behaviors of graphic and tabular notations. From these operations, subgraphs Query, Selection, Merge, Replacement operations can be derived. This approach uses graph PRODUCTIONS (rewriting rules) to study the dynamic transformations of graphs. We discuss the feasibility of implementation these operations. Also, We present the process of porting original TCM (Toolkit for Conceptual Modeling) project from C++ to Java programming language in this thesis. A scenario based on NASA International Space Station Specification is discussed to show the applicability of our approach. Finally

  4. Enterprise SRS: Leveraging Ongoing Operations to Advance National Programs - 13108

    SciTech Connect

    Marra, J.E.; Murray, A.M.; McGuire, P.W.; Wheeler, V.B.

    2013-07-01

    The SRS is re-purposing its vast array of assets to solve future national issues regarding environmental stewardship, national security, and clean energy. The vehicle for this transformation is Enterprise SRS which presents a new, strategic view of SRS as a united endeavor for 'all things nuclear' as opposed to a group of distinct and separate entities with individual missions and organizations. Key among the Enterprise SRS strategic initiatives is the integration of research into facilities in conjunction with ongoing missions to provide researchers from other national laboratories, academic institutions, and commercial entities the opportunity to demonstrate their technologies in a relevant environment and scale prior to deployment. To manage that integration of research demonstrations into site facilities, The DOE Savannah River Operations Office, Savannah River Nuclear Solutions, and the Savannah River National Laboratory (SRNL) have established the Center for Applied Nuclear Materials Processing and Engineering Research (CANMPER). The key objective of this initiative is to bridge the gap between promising transformational nuclear materials management advancements and large-scale deployment of the technology by leveraging SRS assets (e.g. facilities, staff, and property) for those critical engineering-scale demonstrations necessary to assure the successful deployment of new technologies. CANMPER will coordinate the demonstration of R and D technologies and serve as the interface between the engineering-scale demonstration and the R and D programs, essentially providing cradle-to-grave support to the R and D team during the demonstration. While the initial focus of CANMPER will be on the effective use of SRS assets for these demonstrations, CANMPER also will work with research teams to identify opportunities to perform R and D demonstrations at other facilities. Unique to this approach is the fact that these SRS assets will continue to accomplish DOE's critical

  5. Mitochondria are required for antigen-specific T cell activation through reactive oxygen species signaling

    PubMed Central

    Sena, Laura A.; Li, Sha; Jairaman, Amit; Prakriya, Murali; Ezponda, Teresa; Hildeman, David A.; Wang, Chyung-Ru; Schumacker, Paul T.; Licht, Jonathan D.; Perlman, Harris; Bryce, Paul J.; Chandel, Navdeep S.

    2013-01-01

    SUMMARY It is widely appreciated that T cells increase glycolytic flux during activation, however the role of mitochondrial flux is unclear. Here we have shown that mitochondrial metabolism, in the absence of glucose metabolism, was sufficient to support interleukin-2 (IL-2) induction. Furthermore, we used mice with reduced mitochondrial reactive oxygen species (mROS) production in T cells (T-Uqcrfs−/− mice) to show that mitochondria are required for T cell activation to produce mROS for activation of nuclear factor of activated T cells (NFAT) and subsequent IL-2 induction. These mice could not induce antigen-specific expansion of T cells in vivo, however Uqcrfs1−/− T cells retained the ability to proliferate in vivo under lymphopenic conditions. This suggests that Uqcrfs1−/− T cells were not lacking bioenergetically, but rather lacked specific ROS-dependent signaling events needed for antigen-specific expansion. Thus, mitochondrial metabolism is a critical component of T cell activation through production of complex III ROS. PMID:23415911

  6. NPRA Q and A - 1; 1990 Fuel specifications will require process changes

    SciTech Connect

    Yuan, D.C. ); Soumagne, G.; Siegrist, M.R. )

    1990-02-26

    This paper discusses the alteration of specifications for gasoline and diesel fuels in the 1990:0090s. Higher-octane gasoline with lower vapor pressure, and diesel fuel with lower sulfur and aromatics, will require process changes to produce. These altered fuel specifications prompted several questions at the most recent National Petroleum Refiners Association annual question and answer session on refining and petrochemical technology held in New Orleans, October 4-6, 1989. At this meeting, moderated by Herbert W. Bruch, NPRA's technical director, engineers and managers from refineries and petrochemical plants around the world gather to discuss their operating experiences in the form of questions and answers that cover a wide range of topics. A panel of selected technical representatives of operating companies and suppliers first answer previously submitted questions, then the audience is invited to add its contributions. This excerpt from the transcript of the meeting examines operating conditions and catalysts that increase motor octane number in FCC units, the effects on processes of lower vapor pressure gasoline specifications, and processing routes to low sulfur and aromatic diesel fuels.

  7. Brg1 directly regulates Olig2 transcription and is required for oligodendrocyte progenitor cell specification.

    PubMed

    Matsumoto, Steven; Banine, Fatima; Feistel, Kerstin; Foster, Scott; Xing, Rubing; Struve, Jaime; Sherman, Larry S

    2016-05-15

    The Olig2 basic-helix-loop-helix transcription factor promotes oligodendrocyte specification in early neural progenitor cells (NPCs), including radial glial cells, in part by recruiting SWI/SNF chromatin remodeling complexes to the enhancers of genes involved in oligodendrocyte differentiation. How Olig2 expression is regulated during oligodendrogliogenesis is not clear. Here, we find that the Brg1 subunit of SWI/SNF complexes interacts with a proximal Olig2 promoter and represses Olig2 transcription in the mouse cortex at E14, when oligodendrocyte progenitors (OPCs) are not yet found in this location. Brg1 does not interact with the Olig2 promoter in the E14 ganglionic eminence, where NPCs differentiate into Olig2-positive OPCs. Consistent with these findings, Brg1-null NPCs demonstrate precocious expression of Olig2 in the cortex. However, these cells fail to differentiate into OPCs. We further find that Brg1 is necessary for neuroepithelial-to-radial glial cell transition, but not neuronal differentiation despite a reduction in expression of the pro-neural transcription factor Pax6. Collectively, these and earlier findings support a model whereby Brg1 promotes neurogenic radial glial progenitor cell specification but is dispensable for neuronal differentiation. Concurrently, Brg1 represses Olig2 expression and the specification of OPCs, but is required for OPC differentiation and oligodendrocyte maturation. PMID:27067865

  8. Arx is required for specification of the zona incerta and reticular nucleus of the thalamus.

    PubMed

    Sunnen, C Nicole; Simonet, Jacqueline C; Marsh, Eric D; Golden, Jeffrey A

    2014-03-01

    Mutations in the aristaless-related homeobox (ARX) gene result in a spectrum of structural and functional nervous system disorders including lissencephaly, movement disorders, intellectual disabilities, and epilepsy. Some patients also have symptoms indicating hypothalamic dysfunction, but little is known about the role of ARX in diencephalic development. To begin evaluating diencephalic defects, we examined the expression of a panel of known genes and gene products that label specific diencephalic nuclei in 2 different Arx mutant mouse lines at E18.5. Male mice engineered to have a polyalanine expansion mutation (Arx) revealed no expression differences in any diencephalic nucleus when compared with wild-type littermates. In contrast, mice null for Arx (Arx) lost expression of specific markers of the thalamic reticular nucleus and zona incerta (ZI) while retaining expression in other thalamic nuclei and in the hypothalamus. Tyrosine hydroxylase, a marker of the dopaminergic A13 subnucleus of ZI, was among those lost, suggesting a requirement for Arx in normal thalamic reticular nucleus and ZI development and, specifically, for A13 dopaminergic fate. Because the ZI and A13 regions make connections to several hypothalamic nuclei, such misspecification may contribute to the "hypothalamic dysfunction" observed in some patients. PMID:24487799

  9. Australin: a chromosomal passenger protein required specifically for Drosophila melanogaster male meiosis

    PubMed Central

    Gao, Shan; Giansanti, Maria Grazia; Buttrick, Graham J.; Ramasubramanyan, Sharada; Auton, Adam; Gatti, Maurizio; Wakefield, James G.

    2008-01-01

    The chromosomal passenger complex (CPC), which is composed of conserved proteins aurora B, inner centromere protein (INCENP), survivin, and Borealin/DASRA, localizes to chromatin, kinetochores, microtubules, and the cell cortex in a cell cycle–dependent manner. The CPC is required for multiple aspects of cell division. Here we find that Drosophila melanogaster encodes two Borealin paralogues, Borealin-related (Borr) and Australin (Aust). Although Borr is a passenger in all mitotic tissues studied, it is specifically replaced by Aust for the two male meiotic divisions. We analyzed aust mutant spermatocytes to assess the effects of fully inactivating the Aust-dependent functions of the CPC. Our results indicate that Aust is required for sister chromatid cohesion, recruitment of the CPC to kinetochores, and chromosome alignment and segregation but not for meiotic histone phosphorylation or spindle formation. Furthermore, we show that the CPC is required earlier in cytokinesis than previously thought; cells lacking Aust do not initiate central spindle formation, accumulate anillin or actin at the cell equator, or undergo equatorial constriction. PMID:18268101

  10. Point design targets, specifications, and requirements for the 2010 ignition campaign on the National Ignition Facility

    NASA Astrophysics Data System (ADS)

    Haan, S. W.; Lindl, J. D.; Callahan, D. A.; Clark, D. S.; Salmonson, J. D.; Hammel, B. A.; Atherton, L. J.; Cook, R. C.; Edwards, M. J.; Glenzer, S.; Hamza, A. V.; Hatchett, S. P.; Herrmann, M. C.; Hinkel, D. E.; Ho, D. D.; Huang, H.; Jones, O. S.; Kline, J.; Kyrala, G.; Landen, O. L.; MacGowan, B. J.; Marinak, M. M.; Meyerhofer, D. D.; Milovich, J. L.; Moreno, K. A.; Moses, E. I.; Munro, D. H.; Nikroo, A.; Olson, R. E.; Peterson, K.; Pollaine, S. M.; Ralph, J. E.; Robey, H. F.; Spears, B. K.; Springer, P. T.; Suter, L. J.; Thomas, C. A.; Town, R. P.; Vesey, R.; Weber, S. V.; Wilkens, H. L.; Wilson, D. C.

    2011-05-01

    Point design targets have been specified for the initial ignition campaign on the National Ignition Facility [G. H. Miller, E. I. Moses, and C. R. Wuest, Opt. Eng. 443, 2841 (2004)]. The targets contain D-T fusion fuel in an ablator of either CH with Ge doping, or Be with Cu. These shells are imploded in a U or Au hohlraum with a peak radiation temperature set between 270 and 300 eV. Considerations determining the point design include laser-plasma interactions, hydrodynamic instabilities, laser operations, and target fabrication. Simulations were used to evaluate choices, and to define requirements and specifications. Simulation techniques and their experimental validation are summarized. Simulations were used to estimate the sensitivity of target performance to uncertainties and variations in experimental conditions. A formalism is described that evaluates margin for ignition, summarized in a parameter the Ignition Threshold Factor (ITF). Uncertainty and shot-to-shot variability in ITF are evaluated, and sensitivity of the margin to characteristics of the experiment. The formalism is used to estimate probability of ignition. The ignition experiment will be preceded with an experimental campaign that determines features of the design that cannot be defined with simulations alone. The requirements for this campaign are summarized. Requirements are summarized for the laser and target fabrication.

  11. MEI4, a meiosis-specific yeast gene required for chromosome synapsis.

    PubMed Central

    Menees, T M; Ross-MacDonald, P B; Roeder, G S

    1992-01-01

    The MEI4 gene product is required for meiotic induction of recombination and viable spore production in the yeast Saccharomyces cerevisiae. DNA sequence analysis shows that the MEI4 gene encodes a 450-amino-acid protein bearing no homology to any previously identified protein. The MEI4 coding region is interrupted by a small intron located near the 5' end of the gene. Efficient splicing of the MEI4 transcript is not dependent on the MER1 protein, which is required for splicing the transcript of another meiotic gene, MER2. Expression of a mei4::lacZ fusion gene is meiosis-specific and depends on both heterozygosity at the mating-type locus and nutrient limitation. Northern (RNA) blot hybridization analysis suggests that MEI4 gene expression is regulated at the level of transcription. A functional MEI4 gene is not required for meiotic induction of transcription of the MER1, MER2, MEK1, RED1, SPO11, or RAD50 gene. Cytological analysis of mei4 mutant strains during meiotic prophase demonstrates that the chromosomes form long axial elements that fail to undergo synapsis. The meiosis II division is delayed in mei4 strains. Images PMID:1545815

  12. Sensitivity requirements of assisted BDS and GPS in specification 3GPP TS 36.171

    NASA Astrophysics Data System (ADS)

    Jin, Xiaoxi; Chen, Xin; Ying, Rendong; Yang, Genke

    2016-01-01

    With the needs of growing location-based service, a more high-performance satellite positioning technology - assisted global navigation satellite system (A-GNSS assisted-GNSS) becomes a new hotspot in area of navigation and positioning. Now, 3GPP has already provided supports for GPS, Galileo, GLONASS and QZSS, SBAS, so standardization work of introduction BDS into 3GPP organization is very imperative. In this paper, we first analysis the performance of GPS L1 C/A with assistant information, then by taking into account the difference between BDS and GPS, including the unique nature of GEO/NGEO satellites' navigation message data length and format, we design the sensitivity requirements of BDS B1 following A-GPS. The results between A-GPS and A-BDS of typical sensitivity test cases are shown in this paper, which show that the suggested sensitivity requirements satisfy the minimum performance requirements under technical specification of 3GPP TS 36.171.

  13. Stereotactic radiosurgery (SRS) in the modern management of patients with brain metastases

    PubMed Central

    Soliman, Hany; Das, Sunit; Larson, David A.; Sahgal, Arjun

    2016-01-01

    Stereotactic radiosurgery (SRS) is an established non-invasive ablative therapy for brain metastases. Early clinical trials with SRS proved that tumor control rates are superior to whole brain radiotherapy (WBRT) alone. As a result, WBRT plus SRS was widely adopted for patients with a limited number of brain metastases (“limited number” customarily means 1-4). Subsequent trials focused on answering whether WBRT upfront was necessary at all. Based on current randomized controlled trials (RCTs) and meta-analyses comparing SRS alone to SRS plus WBRT, adjuvant WBRT results in better intracranial control; however, at the expense of neurocognitive functioning and quality of life. These adverse effects of WBRT may also negatively impact on survival in younger patients. Based on the results of these studies, treatment has shifted to SRS alone in patients with a limited number of metastases. Additionally, RCTs are evaluating the role of SRS alone in patients with >4 brain metastases. New developments in SRS include fractionated SRS for large tumors and the integration of SRS with targeted systemic therapies that cross the blood brain barrier and/or stimulate an immune response. We present in this review the current high level evidence and rationale supporting SRS as the standard of care for patients with limited brain metastases, and emerging applications of SRS. PMID:26848525

  14. 49 CFR 178.345 - General design and construction requirements applicable to Specification DOT 406 (§ 178.346), DOT...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false General design and construction requirements applicable to Specification DOT 406 (§ 178.346), DOT 407 (§ 178.347), and DOT 412 (§ 178.348) cargo tank....345 General design and construction requirements applicable to Specification DOT 406 (§ 178.346),...

  15. 47 CFR 15.323 - Specific requirements for devices operating in the 1920-1930 MHz sub-band.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 1 2011-10-01 2011-10-01 false Specific requirements for devices operating in the 1920-1930 MHz sub-band. 15.323 Section 15.323 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL RADIO FREQUENCY DEVICES Unlicensed Personal Communications Service Devices § 15.323 Specific requirements for devices operating in the...

  16. 47 CFR 95.1121 - Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Specific requirements for wireless medical... SERVICES Wireless Medical Telemetry Service (WMTS) General Provisions § 95.1121 Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427-1432 MHz bands. Due to the...

  17. 47 CFR 95.1121 - Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Specific requirements for wireless medical... SERVICES Wireless Medical Telemetry Service (WMTS) General Provisions § 95.1121 Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427-1432 MHz bands. Due to the...

  18. The Specific Requirements for CR1 Retrotransposition Explain the Scarcity of Retrogenes in Birds.

    PubMed

    Suh, Alexander

    2015-08-01

    Chicken repeat 1 (CR1) retroposons are the most abundant superfamily of transposable elements in the genomes of birds, crocodilians, and turtles. However, CR1 mobilization remains poorly understood. In this article, I document that the diverse CR1 lineages of land vertebrates share a highly conserved hairpin structure and an octamer microsatellite motif at their very 3' ends. Together with the presence of these same motifs in the tails of CR1-mobilized short interspersed elements, this suggests that the minimum requirement for CR1 transcript recognition and retrotransposition is a complex >50-nt structure. Such a highly specific recognition sequence readily explains why CR1-dominated genomes generally contain very few retrogenes. Conversely, the mammalian richness in retrogenes results from CR1 extinction in their early evolution and subsequent establishment of L1 dominance. PMID:26223967

  19. Rapid amyloid fibril formation by a winter flounder antifreeze protein requires specific interaction with ice.

    PubMed

    Dubé, André; Leggiadro, Cindy; Ewart, Kathryn Vanya

    2016-05-01

    A typically α-helical antifreeze protein (wflAFP-6) from winter flounder, Pseudopleuronectes americanus, forms amyloid fibrils during freezing. In this study, the effects of distinct components of the freezing process were examined. Freezing of wflAFP-6 in the presence of template ice was shown to be necessary for rapid conversion to an amyloid conformation. Neither subfreezing temperature nor phase change was sufficient. Thus, specific interaction with the ice surface was essential. The ice-induced formation of amyloid appeared to be unique to this helical antifreeze, it required high concentrations of protein and it occurred over a range of pH values. These results define a method for rapid formation of amyloid by wflAFP-6 on demand under physiological conditions. PMID:27086686

  20. Specific regions within the embryonic midbrain and cerebellum require different levels of FGF signaling during development

    PubMed Central

    Basson, M. Albert; Echevarria, Diego; Ahn, Christina Petersen; Sudarov, Anamaria; Joyner, Alexandra L.; Mason, Ivor J.; Martinez, Salvador; Martin, Gail R.

    2008-01-01

    SUMMARY Development of the prospective midbrain and cerebellum are coordinated by FGF ligands produced by the isthmic organizer. Previous studies have suggested that the midbrain and cerebellum require different levels of FGF signaling for their development. However, little is known about the extent to which specific regions within these two parts of the brain differ in their requirement for FGF signaling during embryogenesis. In this study, we have explored the effects of inhibiting FGF signaling within the embryonic midbrain (mesencephalon) and cerebellum (rhombomere 1) by misexpressing Sprouty2 (Spry2) specifically in the mouse mesencephalon and rhombomere 1 from an early stage. We show that such Spry2 misexpression moderately reduces FGF signaling, and that this reduction causes the death of cells in the anterior mesencephalon, the region furthest from the source of FGF ligands. Interestingly, the remaining cells in the posterior mesencephalon develop into anterior midbrain, indicating that a low level of FGF signaling is sufficient to promote only anterior midbrain development. Spry2 misexpression also affects development of the vermis, the medial part of the cerebellum that spans the midline. We found that whereas misexpression of Spry2 alone caused loss of the anterior vermis, reducing FGF signaling further, by decreasing Fgf8 gene dosage, resulted in loss of the entire vermis. We provide evidence that cell death is not responsible for this tissue loss. Instead, our data suggest that the vermis fails to develop because reducing FGF signaling perturbs the balance between vermis and roof plate development in rhombomere 1. We suggest a molecular explanation for this phenomenon by providing evidence that FGF signaling functions to inhibit the BMP signaling that promotes roof plate development. PMID:18216176

  1. Environmental Molecular Sciences Laboratory Operations System: Version 4.0 - system requirements specification

    SciTech Connect

    Kashporenko, D.

    1996-07-01

    This document is intended to provide an operations standard for the Environmental Molecular Sciences Laboratory OPerations System (EMSL OPS). It is directed toward three primary audiences: (1) Environmental Molecular Sciences Laboratory (EMSL) facility and operations personnel; (2) laboratory line managers and staff; and (3) researchers, equipment operators, and laboratory users. It is also a statement of system requirements for software developers of EMSL OPS. The need for a finely tuned, superior research environment as provided by the US Department of Energy`s (DOE) Environmental Molecular Sciences Laboratory has never been greater. The abrupt end of the Cold War and the realignment of national priorities caused major US and competing overseas laboratories to reposition themselves in a highly competitive research marketplace. For a new laboratory such as the EMSL, this means coming into existence in a rapidly changing external environment. For any major laboratory, these changes create funding uncertainties and increasing global competition along with concomitant demands for higher standards of research product quality and innovation. While more laboratories are chasing fewer funding dollars, research ideas and proposals, especially for molecular-level research in the materials and biological sciences, are burgeoning. In such an economically constrained atmosphere, reduced costs, improved productivity, and strategic research project portfolio building become essential to establish and maintain any distinct competitive advantage. For EMSL, this environment and these demands require clear operational objectives, specific goals, and a well-crafted strategy. Specific goals will evolve and change with the evolution of the nature and definition of DOE`s environmental research needs. Hence, EMSL OPS is designed to facilitate migration of these changes with ease into every pertinent job function, creating a facile {open_quotes}learning organization.{close_quotes}

  2. Simulating NIF laser-plasma interaction with multiple SRS frequencies

    SciTech Connect

    Still, C H; Hinkel, D E; Langdon, A B; Palastro, J P; Williams, E A

    2009-10-05

    Understanding the energetics of a NIF ignition hohlraum is important to achieving ignition. Laser-plasma interactions (LPI) can reduce the radiation drive if backscatter occurs, and can also affect the hohlraum energetics by modifying the laser beam energy deposition which in turn can alter the implosion symmetry. The addition of a second SRS frequency to the modeling code pF3d can capture physics which would otherwise have been omitted. In the case of a wide or bi-modal SRS spectrum, this physics can be important. We discuss the modifications to the pF3d computational model, and exhibit its effect in a NIF ignition-relevant LPI simulation.

  3. Evaluation of Background Mercury Concentrations in the SRS Groundwater System

    SciTech Connect

    Looney, B.B.

    1999-03-03

    Mercury analyses associated with the A-01 Outfall have highlighted the importance of developing an understanding of mercury in the Savannah River Site groundwater system and associated surface water streams. This activity is critical based upon the fact that the EPA Ambient Water Quality Criteria (AWQC) for this constituent is 0.012mg/L, a level that is well below conventional detection limits of 0.1 to 0.2 mg/L. A first step in this process is obtained by utilizing the existing investment in groundwater mercury concentrations (20,242 records) maintained in the SRS geographical information management system (GIMS) database. Careful use of these data provides a technically defensible initial estimate for total recoverable mercury in background and contaminated SRS wells.

  4. Sex specific retinoic acid signaling is required for the initiation of urogenital sinus bud development.

    PubMed

    Bryant, Sarah L; Francis, Jeffrey C; Lokody, Isabel B; Wang, Hong; Risbridger, Gail P; Loveland, Kate L; Swain, Amanda

    2014-11-15

    The mammalian urogenital sinus (UGS) develops in a sex specific manner, giving rise to the prostate in the male and the sinus vagina in the embryonic female. Androgens, produced by the embryonic testis, have been shown to be crucial to this process. In this study we show that retinoic acid signaling is required for the initial stages of bud development from the male UGS. Enzymes involved in retinoic acid synthesis are expressed in the UGS mesenchyme in a sex specific manner and addition of ligand to female tissue is able to induce prostate-like bud formation in the absence of androgens, albeit at reduced potency. Functional studies in mouse organ cultures that faithfully reproduce the initiation of prostate development indicate that one of the roles of retinoic acid signaling in the male is to inhibit the expression of Inhba, which encodes the βA subunit of Activin, in the UGS mesenchyme. Through in vivo genetic analysis and culture studies we show that inhibition of Activin signaling in the female UGS leads to a similar phenotype to that of retinoic acid treatment, namely bud formation in the absence of androgens. Our data also reveals that both androgens and retinoic acid have extra independent roles to that of repressing Activin signaling in the development of the prostate during fetal stages. This study identifies a novel role for retinoic acid as a mesenchymal factor that acts together with androgens to determine the position and initiation of bud development in the male UGS epithelia. PMID:25261715

  5. Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification

    PubMed Central

    Boube, Muriel; Faucher, Christian; Joulia, Laurent; Cribbs, David L.; Bourbon, Henri-Marc

    2000-01-01

    The origins of specificity in gene expression are a central concern in understanding developmental control. Mediator protein complexes regulate transcriptional initiation, acting as modular adaptors linking specific transcription factors to core RNA polymerase II. Here, we identified the Drosophila homologs of 23 human mediator genes and mutations of two, dTRAP240 and of dTRAP80 (the putative fly homolog of yeast SRB4). Clonal analysis indicates a general role for dTRAP80 necessary for cell viability. The dTRAP240 gene is also essential, but cells lacking its function are viable and proliferate normally. Clones reveal localized developmental activities including a sex comb cell identity function. This contrasts with the ubiquitous nuclear accumulation of dTRAP240 protein in imaginal discs. Synergistic genetic interactions support shared developmental cell and segment identity functions of dTRAP240 and dTRAP80, potentially within a common complex. Further, they identify the homeotic Sex combs reduced product, required for the same cell/tissue identities, as a functional partner of these mediator proteins. PMID:11090137

  6. Sequence requirement for specific interaction of an enhancer binding protein (EBP1) with DNA.

    PubMed Central

    Clark, L; Hay, R T

    1989-01-01

    Short DNA sequence motifs have been identified in viral and cellular enhancers which represent the binding sites for a variety of trans- acting factors. One such HeLa cell factor, EBP1, has been purified and shown to bind to sequences in the SV40 enhancer. The PRDII element in the human beta-interferon gene regulatory element (IRE) shows strong sequence similarity to the EBP1 binding site in the SV40 enhancer. We demonstrate here that EBP1 binds to its sites in the SV40 enhancer and IRE in a similar manner, making base specific contacts over one complete turn of the DNA double helix. Mutational analysis of the EBP1 sites in the IRE and SV40 enhancer has identified the DNA sequence requirements necessary for specific EBP1/DNA complex formation. In addition, 34 DNA sequences related to the EBP1 binding site were analysed for their ability to bind EBP1. Sequences constituting high affinity binding sites possess the sequence 5'-GG(N)6CC-3'. Single base pair changes in the region between the conserved Gs and Cs can generally be tolerated although it is clear that these intervening bases contribute to binding affinity. Mutations in the recognition site which could lead to gross structural changes in the DNA abolish EBP1 binding. Images PMID:2536920

  7. A Symmetrical, Planar SOFC Design for NASA's High Specific Power Density Requirements

    NASA Technical Reports Server (NTRS)

    Cable, Thomas L.; Sofie, Stephen W.

    2007-01-01

    Solid oxide fuel cell (SOFC) systems for aircraft applications require an order of magnitude increase in specific power density (1.0 kW/kg) and long life. While significant research is underway to develop anode supported cells which operate at temperatures in the range of 650-800 C, concerns about Cr-contamination from the metal interconnect may drive the operating temperature down further, to 750 C and lower. Higher temperatures, 900-1000 C, are more favorable for SOFC stacks to achieve specific power densities of 1.0 kW/kg. Since metal interconnects are not practical at these high temperatures and can account for up to 75% of the weight of the stack, NASA is pursuing a design that uses a thin, LaCrO3-based ceramic interconnect that incorporates gas channels into the electrodes. The bi-electrode supported cell (BSC) uses porous YSZ scaffolds, on either side of a 10-20 microns electrolyte. The porous support regions are fabricated with graded porosity using the freeze-tape casting process which can be tailored for fuel and air flow. Removing gas channels from the interconnect simplifies the stack design and allows the ceramic interconnect to be kept thin, on the order of 50 -100 microns. The YSZ electrode scaffolds are infiltrated with active electrode materials following the high temperature sintering step. The NASA-BSC is symmetrical and CTE matched, providing balanced stresses and favorable mechanical properties for vibration and thermal cycling.

  8. The positive transcriptional elongation factor (P-TEFb) is required for neural crest specification.

    PubMed

    Hatch, Victoria L; Marin-Barba, Marta; Moxon, Simon; Ford, Christopher T; Ward, Nicole J; Tomlinson, Matthew L; Desanlis, Ines; Hendry, Adam E; Hontelez, Saartje; van Kruijsbergen, Ila; Veenstra, Gert Jan C; Münsterberg, Andrea E; Wheeler, Grant N

    2016-08-15

    Regulation of gene expression at the level of transcriptional elongation has been shown to be important in stem cells and tumour cells, but its role in the whole animal is only now being fully explored. Neural crest cells (NCCs) are a multipotent population of cells that migrate during early development from the dorsal neural tube throughout the embryo where they differentiate into a variety of cell types including pigment cells, cranio-facial skeleton and sensory neurons. Specification of NCCs is both spatially and temporally regulated during embryonic development. Here we show that components of the transcriptional elongation regulatory machinery, CDK9 and CYCLINT1 of the P-TEFb complex, are required to regulate neural crest specification. In particular, we show that expression of the proto-oncogene c-Myc and c-Myc responsive genes are affected. Our data suggest that P-TEFb is crucial to drive expression of c-Myc, which acts as a 'gate-keeper' for the correct temporal and spatial development of the neural crest. PMID:27343897

  9. Hybrid Microwave Treatment of SRS TRU and Mixed Wastes

    SciTech Connect

    Wicks, G.G.

    1999-11-18

    A new process, using hybrid microwave energy, has been developed as part of the Strategic Research and Development program and successfully applied to treatment of a wide variety of non-radioactive materials, representative of SRS transuranic (TRU) and mixed wastes. Over 35 simulated (non-radioactive) TRU and mixed waste materials were processed individually, as well as in mixed batches, using hybrid microwave energy, a new technology now being patented by Westinghouse Savannah River Company (WSRC).

  10. 10 CFR 33.14 - Requirements for the issuance of a Type B specific license of broad scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Requirements for the issuance of a Type B specific license... issuance of a Type B specific license of broad scope. An application for a Type B specific license of broad... officer who is qualified by training and experience in radiation protection, and who is available...

  11. 10 CFR 33.13 - Requirements for the issuance of a Type A specific license of broad scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Requirements for the issuance of a Type A specific license... issuance of a Type A specific license of broad scope. An application for a Type A specific license of broad... that are necessary to assure safe operations, including: (1) The establishment of a radiation...

  12. Direct effects of ionizing radiation on integral membrane proteins. Noncovalent energy transfer requires specific interpeptide interactions

    SciTech Connect

    Jhun, E.; Jhun, B.H.; Jones, L.R.; Jung, C.Y. )

    1991-05-25

    The 12 transmembrane alpha helices (TMHs) of human erythrocyte glucose transporter were individually cut by pepsin digestion as membrane-bound 2.5-3.5-kDa peptide fragments. Radiation-induced chemical degradation of these fragments showed an average target size of 34 kDa. This is 10-12 x larger than the average size of an individual TMH, demonstrating that a significant energy transfer occurs among these TMHs in the absence of covalent linkage. Heating this TMH preparation at 100{degree}C for 15 min reduced the target size to 5 kDa or less, suggesting that the noncovalent energy transfer requires specific helix-helix interactions. Purified phospholamban, a small (6-kDa) integral membrane protein containing a single TMH, formed a pentameric assembly in sodium dodecyl sulfate. The chemical degradation target size of this phospholamban pentamer was 5-6 kDa, illustrating that not all integral membrane protein assemblies permit intersubunit energy transfer. These findings together with other published observations suggest strongly that significant noncovalent energy transfer can occur within the tertiary and quaternary structure of membrane proteins and that as yet undefined proper molecular interactions are required for such covalent energy transfer. Our results with pepsin-digested glucose transporter also illustrate the importance of the interhelical interaction as a predominating force in maintaining the tertiary structure of a transmembrane protein.

  13. Specific requirement for ATP at an early step of in vitro transcription of human mitochondrial DNA

    SciTech Connect

    Narasimhan, N.; Attardi, G.

    1987-06-01

    The ATP concentrations allowing transcription of heavy- and light-strand of human mtDNA in a HeLa cell mitochrondrial lysate were found to cover a broad range, with a maximum around 2.5 mM, and with reproducible differences in the ATP response curves for the two transcription events. Direct measurements showed that nonspecific ATP degradation during the assay did not account for the high ATP requirement. 5'-Adenylyl imidodiphosphate (p(NH)ppA), an ATP analog with a nonhydrolyzable ..beta..-..gamma.. bond, was unable to substitute for ATP in supporting mtDNA transcription but greatly stimulated this transcription in the presence of a low concentration of exogenous APT, measured with (/sup 32/P)-labeled nucleotides. Evidence was obtained indicating that p(NH)ppA did not support an early event in mtDNA transcription (formation of preinitiation complex or initiation), whereas this analog could substitute effectively for ATP in the subsequent elongation steps. These results pointed to a specific requirement for ATP at an early step of the transcription process.

  14. Root hair-specific EXPANSIN A7 is required for root hair elongation in Arabidopsis.

    PubMed

    Lin, Changfa; Choi, Hee-Seung; Cho, Hyung-Taeg

    2011-04-01

    Expansins are non-hydrolytic cell wall-loosening proteins that are involved in the cell wall modifications that underlie many plant developmental processes. Root hair growth requires the accumulation of cell wall materials and dynamic cell wall modification at the tip region. Although several lines of indirect evidence support the idea that expansin-mediated wall modification occurs during root hair growth, the involvement of these proteins remains to be demonstrated in vivo. In this study, we used RNA interference (RNAi) to examine the biological function of Arabidopsis thaliana EXPANSIN A7 (AtEXPA7), which is expressed specifically in the root hair cell. The root hairspecific AtEXPA7 promoter was used to drive RNAi expression, which targeted two independent regions in the AtEXPA7 transcript. Quantitative reverse transcriptase-PCR analyses were used to examine AtEXPA7 transcript levels. In four independent RNAi transformant lines, RNAi expression reduced AtEXPA7 transcript levels by 25-58% compared to controls. Accordingly, the root hairs of RNAi transformant lines were 25-48% shorter than control plants and exhibited a broader range of lengths than the controls. Our results provide in vivo evidence that expansins are required for root hair tip growth. PMID:21359675

  15. Controlling instabilities in manipulation requires specific cortical-striatal-cerebellar networks

    PubMed Central

    Lau, Chad; Wang, Yang; Venkadesan, Madhusudhan; Valero-Cuevas, Francisco J.

    2011-01-01

    Dexterous manipulation requires both strength, the ability to produce fingertip forces of a specific magnitude, and dexterity, the ability to dynamically regulate the magnitude and direction of fingertip force vectors and finger motions. Although cortical activity in fronto-parietal networks has been established for stable grip and pinch forces, the cortical regulation in the dexterous control of unstable objects remains unknown. We used functional magnetic resonance imaging (fMRI) to interrogate cortical networks engaged in the control of four objects with increasing instabilities but requiring constant strength. In addition to expected activity in fronto-parietal networks we find that dexterous manipulation of increasingly unstable objects is associated with a linear increase in the amplitude of the BOLD signal in the basal ganglia (P = 0.007 and P = 0.023 for 2 compression tasks). A computational regression (connectivity) model identified independent subsets of cortical networks whose connection strengths were mutable and associated with object instability (P < 0.001). Our results suggest that in the presence of object instability, the basal ganglia may modulate the activity of premotor areas and subsequent motor output. This work, therefore, provides new evidence for the selectable cortical representation and execution of dynamic multifinger manipulation for grasp stability. PMID:21228301

  16. Differential requirements for Gli2 and Gli3 in the regional specification of the mouse hypothalamus

    PubMed Central

    Haddad-Tóvolli, Roberta; Paul, Fabian A.; Zhang, Yuanfeng; Zhou, Xunlei; Theil, Thomas; Puelles, Luis; Blaess, Sandra; Alvarez-Bolado, Gonzalo

    2015-01-01

    Secreted protein Sonic hedgehog (Shh) ventralizes the neural tube by modulating the crucial balance between activating and repressing functions (GliA, GliR) of transcription factors Gli2 and Gli3. This balance—the Shh-Gli code—is species- and context-dependent and has been elucidated for the mouse spinal cord. The hypothalamus, a forebrain region regulating vital functions like homeostasis and hormone secretion, shows dynamic and intricate Shh expression as well as complex regional differentiation. Here we asked if particular combinations of Gli2 and Gli3 and of GliA and GliR functions contribute to the variety of hypothalamic regions, i.e., we wanted to approach the question of a possible hypothalamic version of the Shh-Gli code. Based on mouse mutant analysis, we show that: (1) hypothalamic regional heterogeneity is based in part on differentially stringent requirements for Gli2 or Gli3; (2) another source of diversity are differential requirements for Shh of neural vs. non-neural origin; (3) the medial progenitor domain known to depend on Gli2 for its development generates several essential hypothalamic nuclei plus the pituitary and median eminence; (4) the suppression of Gli3R by neural and non-neural Shh is essential for hypothalamic specification. Finally, we have mapped our results on a recent model which considers the hypothalamus as a transverse region with alar and basal portions. Our data confirm the model and are explained by it. PMID:25859185

  17. Specific Requirements of Physiotherapists on the Practical Use of Software in the Therapeutical Process.

    PubMed

    Messer-Misak, Karin; Egger, Rudolf

    2016-01-01

    The current healthcare system requires more effective management. New media and technology are supposed to support the demands of the current healthcare system. By the example of physiotherapy, the primary objective of this study was to define the specific requirements of therapists on the practical use of software which cover the administration, documentation and evaluation of the entire therapy process, including a database with pictures/videos about exercises which can be adapted individually by the therapists. Another objective was to show what conditions for a successful implementation of advanced applications during the entire treatment process have to be fulfilled. The approach of mixed-methods designs was chosen. In the first part a two-stage qualitative study was carried out, followed by a quantitative survey. The results show that the use of the software regarding the therapy-related part is dependent on how adaptable the software is to the special needs of the therapists, that the whole treatment process is mapped on the software and that an additional training during the professional practice must be implemented in order to deploy the use of the software successfully in the therapeutic process. PMID:27139399

  18. Engineering Specification for Large-aperture UVO Space Telescopes Derived from Science Requirements

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip; Postman, Mark; Smith, W. Scott

    2013-01-01

    The Advance Mirror Technology Development (AMTD) project is a three year effort initiated in FY12 to mature by at least a half TRL step six critical technologies required to enable 4 to 8 meter UVOIR space telescope primary mirror assemblies for both general astrophysics and ultra-high contrast observations of exoplanets. AMTD uses a science-driven systems engineering approach. We mature technologies required to enable the highest priority science AND result in a high-performance low-cost low-risk system. To provide the science community with options, we are pursuing multiple technology paths. We have assembled an outstanding team from academia, industry, and government with extensive expertise in astrophysics and exoplanet characterization, and in the design/manufacture of monolithic and segmented space telescopes. A key accomplishment is deriving engineering specifications for advanced normal-incidence monolithic and segmented mirror systems needed to enable both general astrophysics and ultra-high contrast observations of exoplanets missions as a function of potential launch vehicles and their mass and volume constraints.

  19. Tolerance induction in memory CD4 T cells requires two rounds of antigen-specific activation.

    PubMed

    David, Alexandria; Crawford, Frances; Garside, Paul; Kappler, John W; Marrack, Philippa; MacLeod, Megan

    2014-05-27

    A major goal for immunotherapy is to tolerize the immune cells that coordinate tissue damage in autoimmune and alloantigen responses. CD4 T cells play a central role in many of these conditions and improved antigen-specific regulation or removal of these cells could revolutionize current treatments. A confounding factor is that little is known about whether and how tolerance is induced in memory CD4 T cells. We used MHC class II tetramers to track and analyze a population of endogenous antigen-specific memory CD4 T cells exposed to soluble peptide in the absence of adjuvant. We found that such memory T cells proliferated and reentered the memory pool apparently unperturbed by the incomplete activation signals provided by the peptide. Upon further restimulation in vivo, CD4 memory T cells that had been previously exposed to peptide proliferated, provided help to primary responding B cells, and migrated to inflamed sites. However, these reactivated memory cells failed to survive. The reduction in T-cell number was marked by low expression of the antiapoptotic molecule B cell lymphoma 2 (Bcl2) and increased expression of activated caspase molecules. Consequently, these cells failed to sustain a delayed-type hypersensitivity response. Moreover, following two separate exposures to soluble antigen, no T-cell recall response and no helper activity for B cells could be detected. These results suggest that the induction of tolerance in memory CD4 T cells is possible but that deletion and permanent removal of the antigen-specific T cells requires reactivation following exposure to the tolerogenic antigen. PMID:24821788

  20. A domain in the transcription activator Gln3 specifically required for rapamycin responsiveness.

    PubMed

    Rai, Rajendra; Tate, Jennifer J; Shanmuganatham, Karthik; Howe, Martha M; Cooper, Terrance G

    2014-07-01

    Nitrogen-responsive control of Gln3 localization is implemented through TorC1-dependent (rapamycin-responsive) and TorC1-independent (nitrogen catabolite repression-sensitive and methionine sulfoximine (Msx)-responsive) regulatory pathways. We previously demonstrated amino acid substitutions in a putative Gln3 α-helix(656-666), which are required for a two-hybrid Gln3-Tor1 interaction, also abolished rapamycin responsiveness of Gln3 localization and partially abrogated cytoplasmic Gln3 sequestration in cells cultured under nitrogen-repressive conditions. Here, we demonstrate these three characteristics are not inextricably linked together. A second distinct Gln3 region (Gln3(510-589)) is specifically required for rapamycin responsiveness of Gln3 localization, but not for cytoplasmic Gln3 sequestration under repressive growth conditions or relocation to the nucleus following Msx addition. Aspartate or alanine substitution mutations throughout this region uniformly abolish rapamycin responsiveness. Contained within this region is a sequence with a predicted propensity to form an α-helix(583-591), one side of which consists of three hydrophobic amino acids flanked by serine residues. Substitution of aspartate for even one of these serines abolishes rapamycin responsiveness and increases rapamycin resistance without affecting either of the other two Gln3 localization responses. In contrast, alanine substitutions decrease rapamycin resistance. Together, these data suggest that targets in the C-terminal portion of Gln3 required for the Gln3-Tor1 interaction, cytoplasmic Gln3 sequestration, and Gln3 responsiveness to Msx addition and growth in poor nitrogen sources are distinct from those needed for rapamycin responsiveness. PMID:24847055

  1. Functional requirements analysis and human machine interface specifications for handheld metal detector wands

    SciTech Connect

    Hoang, V.; Hartney, C.; Banks, W.

    1994-11-01

    Functional Requirements Analysis (FRA) and Human-Machine-Interface Design Specifications (HMIDs) are critical elements in the development of effective security systems. Handheld metal detector wands are currently used by security personnel to detect metal weapons and munitions that might be smuggled onboard an aircraft by terrorists or individuals who intend to do harm to passengers, aircraft, or other air carrier-related targets. The FAA has requested that Lawrence Livermore National Laboratory (LLNL) assist in developing functional requirements for handheld metal detector devices (wands) used at airports. This effort is focused on both defining and assuring adequate functional and human interface designs that are an integral part of airport security operations. In addition to developing functional requirements, LLNL was also requested to examine and review wanding procedures currently used by the airports and air carriers and provide comments, recommendations, and suggestions for enhanced security based upon this review. The phrase ``Human-Machine-Interface`` (HMI) is frequently used to describe the characteristics of a system that allows the human to interact and control the machine or system. Equipment used by checkpoint security Pre-Board Screeners (PBS`s) during rapid search of passengers must be designed to fit a broad range of anthropometric differences in height, hand size, grip strength, upper body strength, visual. acuity, auditory acuity, and other related human variables. In essence, if there is a high degree of compatibility between the end-user and the equipment, there will be a direct enhancement of total system performance and system operability. Thus, this document may also be used as, a guideline to enhance ergonomic compatibility between the PBS`s and the equipment they use.

  2. THERMAL MODELING ANALYSIS OF SRS 70 TON CASK

    SciTech Connect

    Lee, S.; Jordan, J.; Hensel, S.

    2011-03-08

    The primary objective of this work was to perform the thermal calculations to evaluate the Material Test Reactor (MTR) fuel assembly temperatures inside the SRS 70-Ton Cask loaded with various bundle powers. MTR fuel consists of HFBR, MURR, MIT, and NIST. The MURR fuel was used to develop a bounding case since it is the fuel with the highest heat load. The results will be provided for technical input for the SRS 70 Ton Cask Onsite Safety Assessment. The calculation results show that for the SRS 70 ton dry cask with 2750 watts total heat source with a maximum bundle heat of 670 watts and 9 bundles of MURR bounding fuel, the highest fuel assembly temperatures are below about 263 C. Maximum top surface temperature of the plastic cover is about 112 C, much lower than its melting temperature 260 C. For 12 bundles of MURR bounding fuel with 2750 watts total heat and a maximum fuel bundle of 482 watts, the highest fuel assembly temperatures are bounded by the 9 bundle case. The component temperatures of the cask were calculated by a three-dimensional computational fluid dynamics approach. The modeling calculations were performed by considering daily-averaged solar heat flux.

  3. Health effects of SRS non-radiological air emissions

    SciTech Connect

    Stewart, J.

    1997-06-16

    This report examines the potential health effects of non radiological emissions to the air resulting from operations at the Savannah River Site (SRS). The scope of this study was limited to the 55 air contaminants for which the US Environmental Protection Agency (EPA) has quantified risk by determining unit risk factors (excess cancer risks) and/or reference concentrations (deleterious non cancer risks). Potential health impacts have been assessed in relation to the maximally exposed individual. This is a hypothetical person who resides for a lifetime at the SRS boundary. The most recent (1994) quality assured SRS emissions data available were used. Estimated maximum site boundary concentrations of the air contaminants were calculated using air dispersion modeling and 24-hour and annual averaging times. For the emissions studied, the excess cancer risk was found to be less than the generally accepted risk level of 1 in 100,000 and, in most cases, was less than 1 in 1,000,000. Deleterious non cancer effects were also found to be very unlikely.

  4. Systems, methods and apparatus for implementation of formal specifications derived from informal requirements

    NASA Technical Reports Server (NTRS)

    Hinchey, Michael G. (Inventor); Rash, James L. (Inventor); Erickson, John D. (Inventor); Gracinin, Denis (Inventor); Rouff, Christopher A. (Inventor)

    2010-01-01

    Systems, methods and apparatus are provided through which in some embodiments an informal specification is translated without human intervention into a formal specification. In some embodiments the formal specification is a process-based specification. In some embodiments, the formal specification is translated into a high-level computer programming language which is further compiled into a set of executable computer instructions.

  5. Identification of Vaccine Candidate Peptides in the NcSRS2 Surface Protein of Neospora caninum by Using CD4+ Cytotoxic T Lymphocytes and Gamma Interferon-Secreting T Lymphocytes of Infected Holstein Cattle

    PubMed Central

    Staska, Lauren M.; Davies, Christopher J.; Brown, Wendy C.; McGuire, Travis C.; Suarez, Carlos E.; Park, Joo Youn; Mathison, Bruce A.; Abbott, Jeffrey R.; Baszler, Timothy V.

    2005-01-01

    Previously, our laboratory showed that Holstein cattle experimentally infected with Neospora caninum develop parasite-specific CD4+ cytotoxic T lymphocytes (CTL) that lyse infected, autologous target cells through a perforin-granzyme pathway. To identify specific parasite antigens inducing bovine CTL and helper T-lymphocyte responses for vaccine development against bovine neosporosis, the tachyzoite major surface proteins NcSAG1 and NcSRS2 were targeted. In whole tachyzoite antigen-expanded bovine T-lymphocyte lines, recombinant NcSRS2 induced potent memory CD4+- and CD8+-T-lymphocyte activation, as indicated by proliferation and gamma interferon (IFN-γ) secretion, while recombinant NcSAG1 induced a minimal memory response. Subsequently, T-lymphocyte epitope-bearing peptides of NcSRS2 were mapped by using overlapping peptides covering the entire NcSRS2 sequence. Four experimentally infected cattle with six different major histocompatibility complex (MHC) class II haplotypes were the source of immune cells used to identify NcSRS2 peptides presented by Holstein MHC haplotypes. NcSRS2 peptides were mapped by using IFN-γ secretion by rNcSRS2-stimulated, short-term T-lymphocyte cell lines, IFN-γ enzyme-linked immunospot (ELISPOT) assay with peripheral blood mononuclear cells, and 51Cr release cytotoxicity assay of rNcSRS2-stimulated effector cells. Four N. caninum-infected Holstein cattle developed NcSRS2 peptide-specific T lymphocytes detected ex vivo in peripheral blood by IFN-γ ELISPOT and in vitro by measuring T-lymphocyte IFN-γ production and cytotoxicity. An immunodominant region of NcSRS2 spanning amino acids 133 to 155 was recognized by CD4+ T lymphocytes from the four cattle. These findings support investigation of subunit N. caninum vaccines incorporating NcSRS2 gene sequences or peptides for induction of NcSRS2 peptide-specific CTL and IFN-γ-secreting T lymphocytes in cattle with varied MHC genotypes. PMID:15731029

  6. Dietary lipid requirement on non-specific immune responses in juvenile grass carp (Ctenopharyngodon idella).

    PubMed

    Jin, Yan; Tian, Li-xia; Zeng, Shuai-lin; Xie, Shi-wei; Yang, Hui-jun; Liang, Gui-ying; Liu, Yong-jian

    2013-05-01

    This study aimed to evaluate the dietary lipid requirement and its effects on liver oxidative status and non-specific immune responses of juvenile grass carp (Ctenopharyngodon idella). Purified diets with five dietary lipid levels (0%, 2.5%, 5%, 7.5% and 10%, fish oil/corn oil = 1:1) were each fed to triplicate groups of grass carp (mean initial weight: 6.57 ± 0.01 g) in a recirculating rearing system maintained at 27.5 ± 0.5 °C for 10 weeks. Percent weight gain was highest (P < 0.05) with 5% lipid and lowest in fish fed the lipid free control diet. Feed efficiency (FE) and protein efficiency ratio (PER) in fish followed the same pattern of percent weight gain. Fish fed with lipid containing diets had better non-specific immune response indexes (e.g. phagocytic activity, plasma peroxidase and lysozyme activity) and low-level of liver oxidation status than fish fed with the control diet. But excess dietary lipid supplement would bring over metabolic burden to liver. After the feeding trial, fish were challenged by Aeromonas hydrophila. Fish fed control diet obtained significantly (P < 0.05) lower survival rate. The survival rate was highest with 7.5% lipid. The results of this study indicated that proper dietary lipid supplementation enhanced the immune response of grass carp and improved the survival rate in the bacterial challenge, but excess dietary lipid may elevate liver oxidation rates of grass carp. Analysis by second-order regression of percent weight gain indicated that the optimal dietary lipid level in juvenile grass carp (6.6-35.5 g) is about 6.5%. PMID:23416225

  7. Specific NuRD components are required for fin regeneration in zebrafish

    PubMed Central

    2014-01-01

    Background Epimorphic regeneration of a missing appendage in fish and urodele amphibians involves the creation of a blastema, a heterogeneous pool of progenitor cells underneath the wound epidermis. Current evidence indicates that the blastema arises by dedifferentiation of stump tissues in the vicinity of the amputation. In response to tissue loss, silenced developmental programs are reactivated to form a near-perfect copy of the missing body part. However, the importance of chromatin regulation during epimorphic regeneration remains poorly understood. Results We found that specific components of the Nucleosome Remodeling and Deacetylase complex (NuRD) are required for fin regeneration in zebrafish. Transcripts of the chromatin remodeler chd4a/Mi-2, the histone deacetylase hdac1/HDAC1/2, the retinoblastoma-binding protein rbb4/RBBP4/7, and the metastasis-associated antigen mta2/MTA were specifically co-induced in the blastema during adult and embryonic fin regeneration, and these transcripts displayed a similar spatial and temporal expression patterns. In addition, chemical inhibition of Hdac1 and morpholino-mediated knockdown of chd4a, mta2, and rbb4 impaired regenerative outgrowth, resulting in reduction in blastema cell proliferation and in differentiation defects. Conclusion Altogether, our data suggest that specialized NuRD components are induced in the blastema during fin regeneration and are involved in blastema cell proliferation and redifferentiation of osteoblast precursor cells. These results provide in vivo evidence for the involvement of key epigenetic factors in the cellular reprogramming processes occurring during epimorphic regeneration in zebrafish. PMID:24779377

  8. Neuronal activity is required for the development of specific cortical interneuron subtypes.

    PubMed

    De Marco García, Natalia V; Karayannis, Theofanis; Fishell, Gord

    2011-04-21

    Electrical activity has been shown to regulate development in a variety of species and in various structures, including the retina, spinal cord and cortex. Within the mammalian cortex specifically, the development of dendrites and commissural axons in pyramidal cells is activity-dependent. However, little is known about the developmental role of activity in the other major cortical population of neurons, the GABA-producing interneurons. These neurons are morphologically and functionally heterogeneous and efforts over the past decade have focused on determining the mechanisms that contribute to this diversity. It was recently discovered that 30% of all cortical interneurons arise from a relatively novel source within the ventral telencephalon, the caudal ganglionic eminence (CGE). Owing to their late birth date, these interneurons populate the cortex only after the majority of other interneurons and pyramidal cells are already in place and have started to functionally integrate. Here we demonstrate in mice that for CGE-derived reelin (Re)-positive and calretinin (Cr)-positive (but not vasoactive intestinal peptide (VIP)-positive) interneurons, activity is essential before postnatal day 3 for correct migration, and that after postnatal day 3, glutamate-mediated activity controls the development of their axons and dendrites. Furthermore, we show that the engulfment and cell motility 1 gene (Elmo1), a target of the transcription factor distal-less homeobox 1 (Dlx1), is selectively expressed in Re(+) and Cr(+) interneurons and is both necessary and sufficient for activity-dependent interneuron migration. Our findings reveal a selective requirement for activity in shaping the cortical integration of specific neuronal subtypes. PMID:21460837

  9. Niche-Specific Requirement for Hyphal Wall protein 1 in Virulence of Candida albicans

    PubMed Central

    Staab, Janet F.; Datta, Kausik; Rhee, Peter

    2013-01-01

    Specialized Candida albicans cell surface proteins called adhesins mediate binding of the fungus to host cells. The mammalian transglutaminase (TG) substrate and adhesin, Hyphal wall protein 1 (Hwp1), is expressed on the hyphal form of C. albicans where it mediates fungal adhesion to epithelial cells. Hwp1 is also required for biofilm formation and mating thus the protein functions in both fungal-host and self-interactions. Hwp1 is required for full virulence of C. albicans in murine models of disseminated candidiasis and of esophageal candidiasis. Previous studies correlated TG activity on the surface of oral epithelial cells, produced by epithelial TG (TG1), with tight binding of C. albicans via Hwp1 to the host cell surfaces. However, the contribution of other Tgs, specifically tissue TG (TG2), to disseminated candidiasis mediated by Hwp1 was not known. A newly created hwp1 null strain in the wild type SC5314 background was as virulent as the parental strain in C57BL/6 mice, and virulence was retained in C57BL/6 mice deleted for Tgm2 (TG2). Further, the hwp1 null strains displayed modestly reduced virulence in BALB/c mice as did strain DD27-U1, an independently created hwp1Δ/Δ in CAI4 corrected for its ura3Δ defect at the URA3 locus. Hwp1 was still needed to produce wild type biofilms, and persist on murine tongues in an oral model of oropharyngeal candidiasis consistent with previous studies by us and others. Finally, lack of Hwp1 affected the translocation of C. albicans from the mouse intestine into the bloodstream of mice. Together, Hwp1 appears to have a minor role in disseminated candidiasis, independent of tissue TG, but a key function in host- and self-association to the surface of oral mucosa. PMID:24260489

  10. Structural variants of yeast prions show conformer-specific requirements for chaperone activity

    PubMed Central

    Stein, Kevin C.; True, Heather L.

    2016-01-01

    Summary Molecular chaperones monitor protein homeostasis and defend against the misfolding and aggregation of proteins that is associated with protein conformational disorders. In these diseases, a variety of different aggregate structures can form. These are called prion strains, or variants, in prion diseases, and cause variation in disease pathogenesis. Here, we use variants of the yeast prions [RNQ+] and [PSI+] to explore the interactions of chaperones with distinct aggregate structures. We found that prion variants show striking variation in their relationship with Hsp40s. Specifically, the yeast Hsp40 Sis1, and its human ortholog Hdj1, had differential capacities to process prion variants, suggesting that Hsp40 selectivity has likely changed through evolution. We further show that such selectivity involves different domains of Sis1, with some prion conformers having a greater dependence on particular Hsp40 domains. Moreover, [PSI+] variants were more sensitive to certain alterations in Hsp70 activity as compared to [RNQ+] variants. Collectively, our data indicate that distinct chaperone machinery is required, or has differential capacity, to process different aggregate structures. Elucidating the intricacies of chaperone-client interactions, and how these are altered by particular client structures, will be crucial to understanding how this system can go awry in disease and contribute to pathological variation. PMID:25060529

  11. A lophotrochozoan-specific nuclear hormone receptor is required for reproductive system development in the planarian

    PubMed Central

    Tharp, Marla E.; Collins, James J.; Newmark, Phillip A.

    2014-01-01

    Germ cells of sexually reproducing organisms receive an array of cues from somatic tissues that instruct developmental processes. Although the nature of these signals differs amongst organisms, the importance of germline-soma interactions is a common theme. Recently, peptide hormones from the nervous system have been shown to regulate germ cell development in the planarian Schmidtea mediterranea; thus, we sought to investigate a second class of hormones with a conserved role in reproduction, the lipophilic hormones. In order to study these signals, we identified a set of putative lipophilic hormone receptors, known as nuclear hormone receptors, and analyzed their functions in reproductive development. We found one gene, nhr-1, belonging to a small class of functionally uncharacterized lophotrochozoan-specific receptors, to be essential for the development of differentiated germ cells. Upon nhr-1 knockdown, germ cells in the testes and ovaries fail to mature, and remain as undifferentiated germline stem cells. Further analysis revealed that nhr-1 mRNA is expressed in the accessory reproductive organs and is required for their development, suggesting that this transcription factor functions cell non-autonomously in regulating germ cell development. Our studies identify a role for nuclear hormone receptors in planarian reproductive maturation and reinforce the significance of germline-soma interactions in sexual reproduction across metazoans. PMID:25278423

  12. A region-specific neurogenesis mode requires migratory progenitors in the Drosophila visual system

    PubMed Central

    Apitz, Holger; Salecker, Iris

    2014-01-01

    Brain areas each generate specific neuron subtypes during development. However, underlying regional variations in neurogenesis strategies and regulatory mechanisms remain poorly understood. In Drosophila, neurons in four optic lobe ganglia originate from two neuroepithelia, the outer (Opc) and inner (Ipc) proliferation centers. Using genetic manipulations, we demonstrate that one Ipc neuroepithelial domain progressively transforms into migratory progenitors that mature into neural stem cells/neuroblasts within a second domain. Progenitors emerge by an epithelial-mesenchymal transition-like mechanism, requiring the Snail-family member Escargot and, in subdomains, Decapentaplegic signaling. The proneural factors Lethal of scute and Asense differentially control progenitor supply and maturation into neuroblasts. These switch expression from Asense to a third proneural protein, Atonal. Dichaete and Tailless mediate this transition essential for generating two neuron populations at defined positions. We propose that this neurogenesis mode is central for setting-up a new proliferative zone to facilitate spatio-temporal matching of neurogenesis and connectivity across ganglia. PMID:25501037

  13. A region-specific neurogenesis mode requires migratory progenitors in the Drosophila visual system.

    PubMed

    Apitz, Holger; Salecker, Iris

    2015-01-01

    Brain areas each generate specific neuron subtypes during development. However, underlying regional variations in neurogenesis strategies and regulatory mechanisms remain poorly understood. In Drosophila, neurons in four optic lobe ganglia originate from two neuroepithelia, the outer (OPC) and inner (IPC) proliferation centers. Using genetic manipulations, we found that one IPC neuroepithelial domain progressively transformed into migratory progenitors that matured into neural stem cells (neuroblasts) in a second domain. Progenitors emerged by an epithelial-mesenchymal transition-like mechanism that required the Snail-family member Escargot and, in subdomains, Decapentaplegic signaling. The proneural factors Lethal of scute and Asense differentially controlled progenitor supply and maturation into neuroblasts. These switched expression from Asense to a third proneural protein, Atonal. Dichaete and Tailless mediated this transition, which was essential for generating two neuron populations at defined positions. We propose that this neurogenesis mode is central for setting up a new proliferative zone to facilitate spatio-temporal matching of neurogenesis and connectivity across ganglia. PMID:25501037

  14. Description and industrial applications of a standard machine vision system not requiring task-specific development

    NASA Astrophysics Data System (ADS)

    Bruehl, Wolfgang

    1994-11-01

    Modern machine vision systems won't require task specific hard- or software development in most cases. This means for the user short project realization times and minimized cost. Changes or new controls can easily be performed by a trained operator. Hard and software design of such a system is described in this paper, followed by two different applications, which were realized exclusively by menu-guided configuration of the built in set of control methods. The first is an in-line inspection of oven linings. To protect the following machine tool from being damaged, presence of holes and slits have to be controlled. Via serial communication, a host computer gets detailed information about the faulty item and the position of the defect on the lining. The second application describes an in-line inspection of brake pads. To ensure the lining is mounted to the correct side of the carrier, some characters on the carrier side have to be detected. According to the result the system triggers a turn over mechanism.

  15. Thiol redox requirements and substrate specificities of recombinant cytochrome c assembly systems II and III

    PubMed Central

    Richard-Fogal, Cynthia L.; Francisco, Brian San; Frawley, Elaine R.; Kranz, Robert G.

    2011-01-01

    The reconstitution of biosynthetic pathways from heterologous hosts can help define the minimal genetic requirements for pathway function and facilitate detailed mechanistic studies. Each of the three pathways for the assembly of cytochrome c in nature (called systems I, II, and III) has been shown to function recombinantly in Escherichia coli, covalently attaching heme to the cysteine residues of a CXXCH motif of a c-type cytochrome. However, recombinant systems I (CcmABCDEFGH) and II (CcsBA) function in the E. coli periplasm, while recombinant system III (CCHL) attaches heme to its cognate receptor in the cytoplasm of E. coli, which makes direct comparisons between the three systems difficult. Here we show that the human CCHL (with a secretion signal) attaches heme to the human cytochrome c (with a signal sequence) in the E.coli periplasm, which is bioenergetically (p-side) analogous to the mitochondrial intermembrane space. The human CCHL is specific for the human cytochrome c, whereas recombinant system II can attach heme to multiple non-cognate c-type cytochromes (possessing the CXXCH motif.) We also show that the recombinant periplasmic systems II and III use components of the natural E.coli periplasmic DsbC/DsbD thiol-reduction pathway. PMID:21945855

  16. Requirement for interleukin-1 to drive brain inflammation reveals tissue-specific mechanisms of innate immunity

    PubMed Central

    Giles, James A; Greenhalgh, Andrew D; Davies, Claire L; Denes, Adam; Shaw, Tovah; Coutts, Graham; Rothwell, Nancy J; McColl, Barry W; Allan, Stuart M

    2015-01-01

    The immune system is implicated in a wide range of disorders affecting the brain and is, therefore, an attractive target for therapy. Interleukin-1 (IL-1) is a potent regulator of the innate immune system important for host defense but is also associated with injury and disease in the brain. Here, we show that IL-1 is a key mediator driving an innate immune response to inflammatory challenge in the mouse brain but is dispensable in extracerebral tissues including the lung and peritoneum. We also demonstrate that IL-1α is an important ligand contributing to the CNS dependence on IL-1 and that IL-1 derived from the CNS compartment (most likely microglia) is the major source driving this effect. These data reveal previously unknown tissue-specific requirements for IL-1 in driving innate immunity and suggest that IL-1-mediated inflammation in the brain could be selectively targeted without compromising systemic innate immune responses that are important for resistance to infection. This property could be exploited to mitigate injury- and disease-associated inflammation in the brain without increasing susceptibility to systemic infection, an important complication in several neurological disorders. PMID:25367678

  17. SU-E-T-72: Commissioning of a Standardized SRS Cone Set: Determination of the Bolus Gap Factors in a Passively Scattered Proton Beam

    SciTech Connect

    Simpson, R; Gordon, I; Ghebremedhin, A; Wroe, A; Schulte, R; Bush, D; Slater, J; Patyal, B

    2014-06-01

    Purpose: To determine the proton output factors for an SRS cone set using standardized apertures and varied range compensators (bolus blanks); specifically, to determine the best method for modeling the bolus gap factor (BGF) and eliminate the need for patient specific calibrations. Methods: A Standard Imaging A-16 chamber was placed in a Plastic Water phantom to measure the change in dose/MU with different treatment combinations for a proton SRS cone, using standardized apertures and range compensators. Measurements were made with all apertures in the SRS cone set, with four different range compensator thicknesses and five different air gaps between the end of the SRS cone and the surface of the phantom. The chamber was located at iso-center and maintained at a constant depth at the center of modulation for all measurements. Each aperture was placed in the cone to measure the change in MU needed to maintain constant dose at the chamber, as the air gap was increased with different thicknesses of bolus. Results: The dose/MU varied significantly with decreasing aperture size, increasing bolus thickness, or increasing air gap. The measured data was fitted with the lowest order polynomials that accurately described the data, to create a model for determining the change in output for any potential combination of devices used to treat a patient. For a given standardized aperture, the BGF could be described by its constituent factors: the bolus thickness factor (BTF) and the nozzle extension factor (NEF). Conclusion: The methods used to model the dose at the calibration point could be used to accurately predict the change in output for SRS proton beams due to the BGF, eliminating the need for patient specific calibrations. This method for modeling SRS treatments could also be applied to model other treatments using passively scattered proton beams.

  18. Phenotype classification of single cells using SRS microscopy, RNA sequencing, and microfluidics (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Streets, Aaron M.; Cao, Chen; Zhang, Xiannian; Huang, Yanyi

    2016-03-01

    Phenotype classification of single cells reveals biological variation that is masked in ensemble measurement. This heterogeneity is found in gene and protein expression as well as in cell morphology. Many techniques are available to probe phenotypic heterogeneity at the single cell level, for example quantitative imaging and single-cell RNA sequencing, but it is difficult to perform multiple assays on the same single cell. In order to directly track correlation between morphology and gene expression at the single cell level, we developed a microfluidic platform for quantitative coherent Raman imaging and immediate RNA sequencing (RNA-Seq) of single cells. With this device we actively sort and trap cells for analysis with stimulated Raman scattering microscopy (SRS). The cells are then processed in parallel pipelines for lysis, and preparation of cDNA for high-throughput transcriptome sequencing. SRS microscopy offers three-dimensional imaging with chemical specificity for quantitative analysis of protein and lipid distribution in single cells. Meanwhile, the microfluidic platform facilitates single-cell manipulation, minimizes contamination, and furthermore, provides improved RNA-Seq detection sensitivity and measurement precision, which is necessary for differentiating biological variability from technical noise. By combining coherent Raman microscopy with RNA sequencing, we can better understand the relationship between cellular morphology and gene expression at the single-cell level.

  19. Rapid Separation Methods to Characterize Actinides and Metallic Impurities in Plutonium Scrap Materials at SRS

    SciTech Connect

    Maxwell, S.L. III; Jones, V.D.

    1998-07-01

    The Nuclear Materials Stabilization and Storage Division at SRS plans to stabilize selected plutonium scrap residue materials for long term storage by dissolution processing and plans to stabilize other plutonium vault materials via high-temperature furnace processing. To support these nuclear material stabilization activities, the SRS Analytical Laboratories Department (ALD) will provide characterization of materials required prior to the dissolution or the high-firing of these materials. Lab renovations to install new analytical instrumentation are underway to support these activities that include glove boxes with simulated-process dissolution and high- pressure microwave dissolution capability. Inductively-coupled plasma atomic emission spectrometry (ICP-AES), inductively- coupled mass spectrometry (ICP-MS) and thermal-ionization mass spectrometry (TIMS) will be used to measure actinide isotopics and metallic impurities. New high-speed actinide separation methods have been developed that will be applied to isotopic characterization of nuclear materials by TIMS and ICP-MS to eliminate isobaric interferences between Pu-238 /U- 238 and Pu-241/Am-241. TEVA Resin, UTEVA Resin, and TRU Resin columns will be used with vacuum-assisted flow rates to minimize TIMS and ICP-MS sample turnaround times. For metallic impurity analysis, rapid column removal methods using UTEVA Resin, AGMP-1 anion resin and AG MP-50 cation resin have also been developed to remove plutonium and uranium matrix interferences prior to ICP-AES and ICP- MS measurements.

  20. Performance Modeling Applied to the Treatment and Disposal of a Mixed Waste at the SRS

    SciTech Connect

    Pickett, J.B.; Jantzen, C.M.; Cook, J.R.; Whited, A.R.; Field, R.A.

    1997-05-01

    Performance modeling for Low Level Mixed Waste disposal was conducted using the measured leach rates from a number of vitrified waste formulations. The objective of the study was to determine if the improved durability of a vitrified mixed waste would allow trench disposal at the Savannah River Site (SRS). Leaching data were compiled from twenty-nine diverse reference glasses, encompassing a wide range of exposed glass surface area to leachant volume ratios (SA/V), and various leachant solutions; all of which had been leached at 90 degrees Celsius, using the MCC-1 or PCT procedures (ASTM Procedures C1220-92 and C1285-94, respectively). The normalized leach rates were scaled to the ambient disposal temperature of 25 degrees Celsius, and compared to the allowable leach rate of uranium - which would meet the performance assessment requirements. The results indicated that a glass of above average durability (vs. the reference glasses) would meet the uranium leaching concentration for direct SRS trench disposal.

  1. 10 CFR 110.32 - Information required in an application for a specific license/NRC Form 7.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Information required in an application for a specific license/NRC Form 7. 110.32 Section 110.32 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) EXPORT AND IMPORT OF NUCLEAR EQUIPMENT AND MATERIAL Licenses § 110.32 Information required in an application for a specific license/NRC Form 7. (a) Name...

  2. Cytoplasmic nanojunctions between lysosomes and sarcoplasmic reticulum are required for specific calcium signaling.

    PubMed

    Fameli, Nicola; Ogunbayo, Oluseye A; van Breemen, Cornelis; Evans, A Mark

    2014-01-01

    Herein we demonstrate how nanojunctions between lysosomes and sarcoplasmic reticulum (L-SR junctions) serve to couple lysosomal activation to regenerative, ryanodine receptor-mediated cellular Ca (2+) waves. In pulmonary artery smooth muscle cells (PASMCs) it has been proposed that nicotinic acid adenine dinucleotide phosphate (NAADP) triggers increases in cytoplasmic Ca (2+) via L-SR junctions, in a manner that requires initial Ca (2+) release from lysosomes and subsequent Ca (2+)-induced Ca (2+) release (CICR) via ryanodine receptor (RyR) subtype 3 on the SR membrane proximal to lysosomes. L-SR junction membrane separation has been estimated to be < 400 nm and thus beyond the resolution of light microscopy, which has restricted detailed investigations of the junctional coupling process. The present study utilizes standard and tomographic transmission electron microscopy to provide a thorough ultrastructural characterization of the L-SR junctions in PASMCs. We show that L-SR nanojunctions are prominent features within these cells and estimate that the junctional membrane separation and extension are about 15 nm and 300 nm, respectively. Furthermore, we develop a quantitative model of the L-SR junction using these measurements, prior kinetic and specific Ca (2+) signal information as input data. Simulations of NAADP-dependent junctional Ca (2+) transients demonstrate that the magnitude of these signals can breach the threshold for CICR via RyR3. By correlation analysis of live cell Ca (2+) signals and simulated Ca (2+) transients within L-SR junctions, we estimate that "trigger zones" comprising 60-100 junctions are required to confer a signal of similar magnitude. This is compatible with the 110 lysosomes/cell estimated from our ultrastructural observations. Most importantly, our model shows that increasing the L-SR junctional width above 50 nm lowers the magnitude of junctional [Ca (2+)] such that there is a failure to breach the threshold for CICR via RyR3. L

  3. 49 CFR 179.221 - Individual specification requirements applicable to tank car tanks consisting of an inner...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... to tank car tanks consisting of an inner container supported within an outer shell. 179.221 Section... Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.221 Individual specification requirements applicable to tank car tanks consisting of an inner container supported within an outer shell....

  4. 49 CFR 179.221 - Individual specification requirements applicable to tank car tanks consisting of an inner...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... to tank car tanks consisting of an inner container supported within an outer shell. 179.221 Section... Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.221 Individual specification requirements applicable to tank car tanks consisting of an inner container supported within an outer shell....

  5. 49 CFR 179.221 - Individual specification requirements applicable to tank car tanks consisting of an inner...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... to tank car tanks consisting of an inner container supported within an outer shell. 179.221 Section... Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.221 Individual specification requirements applicable to tank car tanks consisting of an inner container supported within an outer shell....

  6. 49 CFR 179.221 - Individual specification requirements applicable to tank car tanks consisting of an inner...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... to tank car tanks consisting of an inner container supported within an outer shell. 179.221 Section... Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.221 Individual specification requirements applicable to tank car tanks consisting of an inner container supported within an outer shell....

  7. Vitrification of actinide solutions in SRS separations facilities

    SciTech Connect

    Minichan, R.L.; Ramsey, W.G.

    1995-09-01

    The actinide vitrification system being developed at SRS provides the capability to convert specialized or unique forms of nuclear material into a stable solid glass product that can be safely shipped, stored or reprocessed according to the DOE complex mission. This project is an application of technology developed through funds from the Office of Technology Development (OTD). This technology is ideally suited for vitrifying relatively small quantities of fissile or special nuclear material since it is designed to be critically safe. Successful demonstration of this system to safely vitrify radioactive material could open up numerous opportunities for transferring this technology to applications throughout the DOE complex.

  8. Requirements Analysis Study for Master Pump Shutdown System Project Development Specification [SEC 1 and 2

    SciTech Connect

    BEVINS, R.R.

    2000-03-24

    This document has been updated during the definitive design portion of the first phase of the W-314 Project to capture additional software requirements and is planned to be updated during the second phase of the W-314 Project to cover the second phase of the Project's scope. The objective is to provide requirement traceability by recording the analysis/basis for the functional descriptions of the master pump shutdown system. This document identifies the sources of the requirements and/or how these were derived. Each requirement is validated either by quoting the source or an analysis process involving the required functionality, performance characteristics, operations input or engineering judgment.

  9. Recommendations for the treatment of aging in standard technical specifications

    SciTech Connect

    Orton, R.D.; Allen, R.P.

    1995-09-01

    As part of the US Nuclear Regulatory Commission`s Nuclear Plant Aging Research Program, Pacific Northwest Laboratory (PNL) evaluated the standard technical specifications for nuclear power plants to determine whether the current surveillance requirements (SRs) were effective in detecting age-related degradation. Nuclear Plant Aging Research findings for selected systems and components were reviewed to identify the stressors and operative aging mechanisms and to evaluate the methods available to detect, differentiate, and trend the resulting aging degradation. Current surveillance and testing requirements for these systems and components were reviewed for their effectiveness in detecting degraded conditions and for potential contributions to premature degradation. When the current surveillance and testing requirements appeared ineffective in detecting aging degradation or potentially could contribute to premature degradation, a possible deficiency in the SRs was identified that could result in undetected degradation. Based on this evaluation, PNL developed recommendations for inspection, surveillance, trending, and condition monitoring methods to be incorporated in the SRs to better detect age- related degradation of these selected systems and components.

  10. SRS MOX fuel lead assemblies data report for the surplus plutonium disposition environmental impact statement

    SciTech Connect

    O`Connor, D.G.; Fisher, S.E.; Holdaway, R.

    1998-08-01

    The purpose of this document is to support the US Department of Energy (DOE) Fissile Materials Disposition Program`s preparation of the draft surplus plutonium disposition environmental impact statement. This is one of several responses to data call requests for background information on activities associated with the operation of the lead assembly (LA) mixed-oxide (MOX) fuel fabrication facility. DOE-MD requested that the DOE Site Operations Offices nominate DOE sites that meet established minimum requirements that could produce MOX LAs. Six initial site combinations were proposed: (1) Argonne National Laboratory-West (ANL-W) with support from Idaho National Engineering and Environmental Laboratory (INEEL), (2) Hanford, (3) Los Alamos National Laboratory (LANL) with support from Pantex, (4) Lawrence Livermore National Laboratory (LLNL), (5) Oak Ridge Reservation (ORR), and (6) Savannah River Site(SRS). After further analysis by the sites and DOE-MD, five site combinations were established as possible candidates for producing MOX LAs: (1) ANL-W with support from INEEL, (2) Hanford, (3) LANL, (4) LLNL, and (5) SRS. SRS has proposed an LA MOX fuel fabrication approach that would be done entirely inside an S and S Category 1 area. An alternate approach would allow fabrication of fuel pellets and assembly of fuel rods in an S and S Category 2 or 3 facility with storage of bulk PuO{sub 2} and assembly, storage, and shipping of fuel bundles in an S and S Category 1 facility. The total Category 1 approach, which is the recommended option, would be done in the 221-H Canyon Building. A facility that was never in service will be removed from one area, and a hardened wall will be constructed in another area to accommodate execution of the LA fuel fabrication. The non-Category 1 approach would require removal of process equipment in the FB-Line metal production and packaging glove boxes, which requires work in a contamination area. The Immobilization Hot Demonstration Program

  11. Enterprise SRS: leveraging ongoing operations to advance nuclear fuel cycles research and development programs

    SciTech Connect

    Murray, A.M.; Marra, J.E.; Wilmarth, W.R.; McGuire, P.W.; Wheeler, V.B.

    2013-07-01

    The Savannah River Site (SRS) is re-purposing its vast array of assets (including H Canyon - a nuclear chemical separation plant) to solve issues regarding advanced nuclear fuel cycle technologies, nuclear materials processing, packaging, storage and disposition. The vehicle for this transformation is Enterprise SRS which presents a new, radical view of SRS as a united endeavor for 'all things nuclear' as opposed to a group of distinct and separate entities with individual missions and organizations. Key among the Enterprise SRS strategic initiatives is the integration of research into SRS facilities but also in other facilities in conjunction with on-going missions to provide researchers from other national laboratories, academic institutions, and commercial entities the opportunity to demonstrate their technologies in a relevant environment and scale prior to deployment. To manage that integration of research demonstrations into site facilities, a center for applied nuclear materials processing and engineering research has been established in SRS.

  12. Requirements Analysis Study for Master Pump Shutdown System Project Development Specification [SEC 1 and 2

    SciTech Connect

    BEVINS, R.R.

    2000-09-20

    This study is a requirements document that presents analysis for the functional description for the master pump shutdown system. This document identifies the sources of the requirements and/or how these were derived. Each requirement is validated either by quoting the source or an analysis process involving the required functionality, performance characteristics, operations input or engineering judgment. The requirements in this study apply to the first phase of the W314 Project. This document has been updated during the definitive design portion of the first phase of the W314 Project to capture additional software requirements and is planned to be updated during the second phase of the W314 Project to cover the second phase of the project's scope.

  13. Some Specific CASL Requirements for Advanced Multiphase Flow Simulation of Light Water Reactors

    SciTech Connect

    R. A. Berry

    2010-11-01

    Because of the diversity of physical phenomena occuring in boiling, flashing, and bubble collapse, and of the length and time scales of LWR systems, it is imperative that the models have the following features: • Both vapor and liquid phases (and noncondensible phases, if present) must be treated as compressible. • Models must be mathematically and numerically well-posed. • The models methodology must be multi-scale. A fundamental derivation of the multiphase governing equation system, that should be used as a basis for advanced multiphase modeling in LWR coolant systems, is given in the Appendix using the ensemble averaging method. The remainder of this work focuses specifically on the compressible, well-posed, and multi-scale requirements of advanced simulation methods for these LWR coolant systems, because without these are the most fundamental aspects, without which widespread advancement cannot be claimed. Because of the expense of developing multiple special-purpose codes and the inherent inability to couple information from the multiple, separate length- and time-scales, efforts within CASL should be focused toward development of a multi-scale approaches to solve those multiphase flow problems relevant to LWR design and safety analysis. Efforts should be aimed at developing well-designed unified physical/mathematical and high-resolution numerical models for compressible, all-speed multiphase flows spanning: (1) Well-posed general mixture level (true multiphase) models for fast transient situations and safety analysis, (2) DNS (Direct Numerical Simulation)-like models to resolve interface level phenmena like flashing and boiling flows, and critical heat flux determination (necessarily including conjugate heat transfer), and (3) Multi-scale methods to resolve both (1) and (2) automatically, depending upon specified mesh resolution, and to couple different flow models (single-phase, multiphase with several velocities and pressures, multiphase with single

  14. Conversion of the SRS to a higher brilliance lattice

    SciTech Connect

    Thompson, D. J.; Suller, V. P.

    1989-07-01

    The SRS at Daresbury has been upgraded by the addition of quadrupoles which reduce the emittance by a factor of 15. In order to do this without disturbing the beamlines, all 16 straight sections were rebuilt. Additional sextupole and octupole magnets were included, as was additional pumping capacity. Completely new beam monitor and vertical steering systems have been incorporated. The SRS was shut down on 1 October 1986 and, after recommissioning, regular scheduled operation for users at the rate of nearly 6000 h per year has been in effect again since June 1987. Scheduled operation is at 2 GeV with the 5T wiggler operational. The gain in brightness is as expected. Maximum beam current at 2 GeV is now 280 mA. Beam lifetime is 20 h at 200 mA. Diffraction patterns of small angle scattering from muscle obtained with an electronic area detector show that with the improved brilliance, diffraction lines corresponding to spacings exceeding 15 000 A can be clearly resolved. Work is about to start on the provision of a second superconducting wiggler with 6T field feeding five new experimental stations.

  15. 40 CFR 62.14620 - What site-specific documentation is required?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That... the incinerator and associated air pollution control systems within the standards established...

  16. Alternative Ultrafiltration Membrane Testing for the SRS Baseline Process

    SciTech Connect

    N. R. Mann; R. S. Herbst; T. G. Garn; M. R. Poirier; S. D. Fink

    2004-06-01

    The ability to more rapidly process high-level waste sludge and supernate, without sacrificing cost savings, continues to be a crucial challenge facing the Savannah River Site (SRS). There has, to date, not been any extensive investigation of alternative filter technologies for the SRS baseline process. To address this problem, a focused investigation into alternative, state-of-the art filtration technologies to facilitate the strontium and actinide removal process, which can be cost effectively implemented in existing facilities and current equipment designs, was completed. Filter technologies manufactured by Mott (0.1 µm and 0.5 µm) Graver (0.07 µm), Pall (0.1 µm and 0.8 µm) and GKN (0.1 µm) were evaluated. Membranes had a nominal inside diameter of 3/8 inches and an active membrane length of 2 feet. The investigation was performed in two phases. The first phase of testing evaluated the consistency or variability in flux through the different membranes using water and a standard 5.0 wt% strontium carbonate slurry. The second phase of testing evaluated the achievable permeate flux and clarity through the various membranes using the SRS average salt supernate simulant at solids loadings of 0.06, 0.29 and 4.5 wt%. Membrane variation data indicate that membranes having an asymmetric ceramic coating (Pall 0.1 µm and Graver 0.07 µm), typically displayed the lowest variability with water. Membranes without a ceramic asymmetric coating (Mott 0.5 µm and GKN 0.1 µm) displayed the highest variability. This is most likely associated with the experimental uncertainties in measuring large volumes of permeate in a short amount of time and to the impact of impurities in the water. In general, variability ranging from 4-56% was observed when using water for all membranes. In the case of variation testing using strontium carbonate, variability decreased to 3-12%. In addition, membrane structure or composition had little effect on the variability. Data obtained from SRS

  17. 23 CFR 230.109 - Implementation of specific Equal Employment Opportunity requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Implementation of specific Equal Employment Opportunity... CIVIL RIGHTS EXTERNAL PROGRAMS Equal Employment Opportunity on Federal and Federal-Aid Construction Contracts (Including Supportive Services) § 230.109 Implementation of specific Equal Employment...

  18. Label-free chemical imaging of live Euglena gracilis by high-speed SRS spectral microscopy (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Wakisaka, Yoshifumi; Suzuki, Yuta; Tokunaga, Kyoya; Hirose, Misa; Domon, Ryota; Akaho, Rina; Kuroshima, Mai; Tsumura, Norimichi; Shimobaba, Tomoyoshi; Iwata, Osamu; Suzuki, Kengo; Nakashima, Ayaka; Goda, Keisuke; Ozeki, Yasuyuki

    2016-03-01

    Microbes, especially microalgae, have recently been of great interest for developing novel biofuels, drugs, and biomaterials. Imaging-based screening of live cells can provide high selectivity and is attractive for efficient bio-production from microalgae. Although conventional cellular screening techniques use cell labeling, labeling of microbes is still under development and can interfere with their cellular functions. Furthermore, since live microbes move and change their shapes rapidly, a high-speed imaging technique is required to suppress motion artifacts. Stimulated Raman scattering (SRS) microscopy allows for label-free and high-speed spectral imaging, which helps us visualize chemical components inside biological cells and tissues. Here we demonstrate high-speed SRS imaging, with temporal resolution of 0.14 seconds, of intracellular distributions of lipid, polysaccharide, and chlorophyll concentrations in rapidly moving Euglena gracilis, a unicellular phytoflagellate. Furthermore, we show that our method allows us to analyze the amount of chemical components inside each living cell. Our results indicate that SRS imaging may be applied to label-free screening of living microbes based on chemical information.

  19. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... qualified and maintained in accordance with the following table. All qualification requirements need not be... thickness, minus 1/2 of the table value in paragraph (g) of this section). D. Condemning limit for general.... Condemning limit for localized corrosion (required minimum shell or head thickness, minus the table value...

  20. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The principal details of... stability. Plans and calculations required by subchapter S of this chapter. (d) Fire control. (1) Fire control diagram showing location and type of all required fire-screen insulation, including main fire...

  1. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The principal details of... stability. Plans and calculations required by subchapter S of this chapter. (d) Fire control. (1) Fire control diagram showing location and type of all required fire-screen insulation, including main fire...

  2. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The principal details of... stability. Plans and calculations required by subchapter S of this chapter. (d) Fire control. (1) Fire control diagram showing location and type of all required fire-screen insulation, including main fire...

  3. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section... procedural requirements under HUD building product standards and certification program for plastic...

  4. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section... procedural requirements under HUD building product standards and certification program for plastic...

  5. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section... procedural requirements under HUD building product standards and certification program for plastic...

  6. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section... procedural requirements under HUD building product standards and certification program for plastic...

  7. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... stability. Plans and calculations required by subchapter S of this chapter. (d) Fire control. (1) Fire control diagram showing location and type of all required fire-screen insulation, including main fire zone... numbers to the “fire control diagram”. (3) Ventilation diagram including dampers and other fire...

  8. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... stability. Plans and calculations required by subchapter S of this chapter. (d) Fire control. (1) Fire control diagram showing location and type of all required fire-screen insulation, including main fire zone... numbers to the “fire control diagram”. (3) Ventilation diagram including dampers and other fire...

  9. Long-term outcome of stereotactic radiosurgery (Srs) in patients with acoustic neuromas

    SciTech Connect

    Combs, Stephanie E. . E-mail: Stephanie.Combs@med.uni-heidelberg.de; Thilmann, Christoph; Debus, Juergen; Schulz-Ertner, Daniela

    2006-04-01

    Purpose: To evaluate the effectiveness and long-term outcome of stereotactic radiosurgery (SRS) for acoustic neuromas (AN). Patients and Methods: Between 1990 and 2001, we treated 26 patients with 27 AN with SRS. Two patients suffered from neurofibromatosis type 2. Before SRS, a subtotal or total resection had been performed in 3 and in 5 patients, respectively. For SRS, a median single dose of 13 Gy/80% isodose was applied. Results: The overall actuarial 5-year and 10-year tumor control probability in all patients was 91%. Two patients developed tumor progression after SRS at 36 and 48 months. Nineteen patients (73%) were at risk of treatment-related facial nerve toxicity; of these, 1 patient developed a complete facial nerve palsy after SRS (5%). A total of 93% of the lesions treated were at risk of radiation-induced trigeminal neuralgia. Two patients (8%) developed mild dysesthesia of the trigeminal nerve after SRS. The hearing preservation rate in patients with useful hearing before SRS was 55% at 9 years. Conclusion:: Stereotactic radiosurgery results in good local control rates of AN and the risk of cranial nerve toxicities is acceptable. As toxicity is lower with fractionated stereotactic radiotherapy, SRS should be reserved for smaller lesions.

  10. rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.

    PubMed

    Cui, X Y; Chen, Z Y; Wu, L; Liu, X Q; Dong, Y Y; Wang, F W; Li, H Y

    2015-01-01

    The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS promoter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter. PMID:26214418

  11. 7 CFR 1724.54 - Requirements for RUS approval of plans and specifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... the application. (See 7 CFR part 1710, subpart F.) Prior to issuing the plans and specifications for... communications systems, load control, and supervisory control and data acquisition (SCADA) systems. (2)...

  12. 49 CFR 180.209 - Requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...; nitrous oxide; oxygen; sulfur hexafluoride; xenon; chlorinated hydrocarbons, fluorinated hydrocarbons... footring or protective ring or fire damage); disposition of cylinder (returned to service, returned to.... (j) Cylinder used as a fire extinguisher. Only a DOT specification cylinder used as a...

  13. 49 CFR 180.209 - Requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...; neon; nitrogen; nitrous oxide; oxygen; sulfur hexafluoride; xenon; chlorinated hydrocarbons... footring or protective ring or fire damage); disposition of cylinder (returned to service, returned to.... (j) Cylinder used as a fire extinguisher. Only a DOT specification cylinder used as a...

  14. 40 CFR 86.306-79 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... for both gasoline-fueled and Diesel engine gaseous emission tests. Generally, the equipment required... Emission Regulations for New Gasoline-Fueled and Diesel-Fueled Heavy-Duty Engines; Gaseous Exhaust...

  15. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... necessary to demonstrate compliance with subpart D of part 130 of this subchapter. (2) Automation-test... or more gross tons, the plans required by § 110.25 of this chapter must be submitted. (f)...

  16. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... necessary to demonstrate compliance with subpart D of part 130 of this subchapter. (2) Automation-test... or more gross tons, the plans required by § 110.25 of this chapter must be submitted. (f)...

  17. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... necessary to demonstrate compliance with subpart D of part 130 of this subchapter. (2) Automation-test... or more gross tons, the plans required by § 110.25 of this chapter must be submitted. (f)...

  18. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... necessary to demonstrate compliance with subpart D of part 130 of this subchapter. (2) Automation-test... or more gross tons, the plans required by § 110.25 of this chapter must be submitted. (f)...

  19. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... necessary to demonstrate compliance with subpart D of part 130 of this subchapter. (2) Automation-test... or more gross tons, the plans required by § 110.25 of this chapter must be submitted. (f)...

  20. Experimental development based on mapping rule between requirements analysis model and web framework specific design model.

    PubMed

    Okuda, Hirotaka; Ogata, Shinpei; Matsuura, Saeko

    2013-12-01

    Model Driven Development is a promising approach to develop high quality software systems. We have proposed a method of model-driven requirements analysis using Unified Modeling Language (UML). The main feature of our method is to automatically generate a Web user interface prototype from UML requirements analysis model so that we can confirm validity of input/output data for each page and page transition on the system by directly operating the prototype. We proposes a mapping rule in which design information independent of each web application framework implementation is defined based on the requirements analysis model, so as to improve the traceability to the final product from the valid requirements analysis model. This paper discusses the result of applying our method to the development of a Group Work Support System that is currently running in our department. PMID:23565356

  1. 40 CFR 86.306-79 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... for both gasoline-fueled and Diesel engine gaseous emission tests. Generally, the equipment required... Emission Regulations for New Gasoline-Fueled and Diesel-Fueled Heavy-Duty Engines; Gaseous Exhaust...

  2. 40 CFR 86.306-79 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... for both gasoline-fueled and Diesel engine gaseous emission tests. Generally, the equipment required... Emission Regulations for New Gasoline-Fueled and Diesel-Fueled Heavy-Duty Engines; Gaseous Exhaust...

  3. 40 CFR 86.306-79 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... for both gasoline-fueled and Diesel engine gaseous emission tests. Generally, the equipment required... Emission Regulations for New Gasoline-Fueled and Diesel-Fueled Heavy-Duty Engines; Gaseous Exhaust...

  4. 49 CFR 173.301a - Additional general requirements for shipment of specification cylinders.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... REGULATIONS SHIPPERS-GENERAL REQUIREMENTS FOR SHIPMENTS AND PACKAGINGS Gases; Preparation and Packaging § 173... effective may be used if the cylinder has been properly tested and otherwise conforms to the...

  5. Waste Receiving and Processing Facility Module 1 Data Management System Software Requirements Specification

    SciTech Connect

    Brann, E.C. II

    1994-09-09

    This document provides the software requirements for Waste Receiving and Processing (WRAP) Module 1 Data Management System (DMS). The DMS is one of the plant computer systems for the new WRAP 1 facility (Project W-026). The DMS will collect, store and report data required to certify the low level waste (LLW) and transuranic (TRU) waste items processed at WRAP 1 as acceptable for shipment, storage, or disposal.

  6. Waste Receiving and Processing Facility Module 1 Data Management System software requirements specification

    SciTech Connect

    Rosnick, C.K.

    1996-04-19

    This document provides the software requirements for Waste Receiving and Processing (WRAP) Module 1 Data Management System (DMS). The DMS is one of the plant computer systems for the new WRAP 1 facility (Project W-0126). The DMS will collect, store and report data required to certify the low level waste (LLW) and transuranic (TRU) waste items processed at WRAP 1 as acceptable for shipment, storage, or disposal.

  7. Thirty-year solid waste generation forecast for facilities at SRS

    SciTech Connect

    Not Available

    1994-07-01

    The information supplied by this 30-year solid waste forecast has been compiled as a source document to the Waste Management Environmental Impact Statement (WMEIS). The WMEIS will help to select a sitewide strategic approach to managing present and future Savannah River Site (SRS) waste generated from ongoing operations, environmental restoration (ER) activities, transition from nuclear production to other missions, and decontamination and decommissioning (D&D) programs. The EIS will support project-level decisions on the operation of specific treatment, storage, and disposal facilities within the near term (10 years or less). In addition, the EIS will provide a baseline for analysis of future waste management activities and a basis for the evaluation of the specific waste management alternatives. This 30-year solid waste forecast will be used as the initial basis for the EIS decision-making process. The Site generates and manages many types and categories of waste. With a few exceptions, waste types are divided into two broad groups-high-level waste and solid waste. High-level waste consists primarily of liquid radioactive waste, which is addressed in a separate forecast and is not discussed further in this document. The waste types discussed in this solid waste forecast are sanitary waste, hazardous waste, low-level mixed waste, low-level radioactive waste, and transuranic waste. As activities at SRS change from primarily production to primarily decontamination and decommissioning and environmental restoration, the volume of each waste s being managed will change significantly. This report acknowledges the changes in Site Missions when developing the 30-year solid waste forecast.

  8. TESTING OF ENHANCED CHEMICAL CLEANING OF SRS ACTUAL WASTE TANK 5F AND TANK 12H SLUDGES

    SciTech Connect

    Martino, C.; King, W.

    2011-08-22

    Forty three of the High Level Waste (HLW) tanks at the Savannah River Site (SRS) have internal structures that hinder removal of the last approximately five thousand gallons of waste sludge solely by mechanical means. Chemical cleaning can be utilized to dissolve the sludge heel with oxalic acid (OA) and pump the material to a separate waste tank in preparation for final disposition. This dissolved sludge material is pH adjusted downstream of the dissolution process, precipitating the sludge components along with sodium oxalate solids. The large quantities of sodium oxalate and other metal oxalates formed impact downstream processes by requiring additional washing during sludge batch preparation and increase the amount of material that must be processed in the tank farm evaporator systems and the Saltstone Processing Facility. Enhanced Chemical Cleaning (ECC) was identified as a potential method for greatly reducing the impact of oxalate additions to the SRS Tank Farms without adding additional components to the waste that would extend processing or increase waste form volumes. In support of Savannah River Site (SRS) tank closure efforts, the Savannah River National Laboratory (SRNL) conducted Real Waste Testing (RWT) to evaluate an alternative to the baseline 8 wt. % OA chemical cleaning technology for tank sludge heel removal. The baseline OA technology results in the addition of significant volumes of oxalate salts to the SRS tank farm and there is insufficient space to accommodate the neutralized streams resulting from the treatment of the multiple remaining waste tanks requiring closure. ECC is a promising alternative to bulk OA cleaning, which utilizes a more dilute OA (nominally 2 wt. % at a pH of around 2) and an oxalate destruction technology. The technology is being adapted by AREVA from their decontamination technology for Nuclear Power Plant secondary side scale removal. This report contains results from the SRNL small scale testing of the ECC process

  9. Tethys: The Marine and Hydrokinetic Technology Environmental Impacts Knowledge Management System -- Requirements Specification -- Version 1.0

    SciTech Connect

    Butner, R. Scott; Snowden-Swan, Lesley J.; Ellis, Peter C.

    2010-11-09

    The marine and hydrokinetic (MHK) environmental impacts knowledge management system (KMS), dubbed Tethys after the mythical Greek goddess of the seas, is being developed for the U.S. Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy Wind and Hydropower Technologies Program (WHTP) by Pacific Northwest National Laboratory (PNNL). This requirements specification establishes the essential capabilities required of Tethys and clarifies for WHTP and the Tethys development team the results that must be achieved by the system.

  10. Tissue-specific DNA demethylation is required for proper B-cell differentiation and function.

    PubMed

    Orlanski, Shari; Labi, Verena; Reizel, Yitzhak; Spiro, Adam; Lichtenstein, Michal; Levin-Klein, Rena; Koralov, Sergei B; Skversky, Yael; Rajewsky, Klaus; Cedar, Howard; Bergman, Yehudit

    2016-05-01

    There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo. PMID:27091986

  11. The LIM Homeodomain Factor Lhx2 Is Required for Hypothalamic Tanycyte Specification and Differentiation

    PubMed Central

    Salvatierra, Juan; Lee, Daniel A.; Zibetti, Cristina; Duran-Moreno, Maria; Yoo, Sooyeon; Newman, Elizabeth A.; Wang, Hong; Bedont, Joseph L.; de Melo, Jimmy; Miranda-Angulo, Ana L.; Gil-Perotin, Sara; Garcia-Verdugo, Jose Manuel

    2014-01-01

    Hypothalamic tanycytes, a radial glial-like ependymal cell population that expresses numerous genes selectively enriched in embryonic hypothalamic progenitors and adult neural stem cells, have recently been observed to serve as a source of adult-born neurons in the mammalian brain. The genetic mechanisms that regulate the specification and maintenance of tanycyte identity are unknown, but are critical for understanding how these cells can act as adult neural progenitor cells. We observe that LIM (Lin-11, Isl-1, Mec-3)-homeodomain gene Lhx2 is selectively expressed in hypothalamic progenitor cells and tanycytes. To test the function of Lhx2 in tanycyte development, we used an intersectional genetic strategy to conditionally delete Lhx2 in posteroventral hypothalamic neuroepithelium, both embryonically and postnatally. We observed that tanycyte development was severely disrupted when Lhx2 function was ablated during embryonic development. Lhx2-deficient tanycytes lost expression of tanycyte-specific genes, such as Rax, while also displaying ectopic expression of genes specific to cuboid ependymal cells, such as Rarres2. Ultrastructural analysis revealed that mutant tanycytes exhibited a hybrid identity, retaining radial morphology while becoming multiciliated. In contrast, postnatal loss of function of Lhx2 resulted only in loss of expression of tanycyte-specific genes. Using chromatin immunoprecipitation, we further showed that Lhx2 directly regulated expression of Rax, an essential homeodomain factor for tanycyte development. This study identifies Lhx2 as a key intrinsic regulator of tanycyte differentiation, sustaining Rax-dependent activation of tanycyte-specific genes while also inhibiting expression of ependymal cell-specific genes. These findings provide key insights into the transcriptional regulatory network specifying this still poorly characterized cell type. PMID:25505333

  12. TECHNICAL BASIS FOR VENTILATION REQUIREMENTS IN TANK FARMS OPERATING SPECIFICATIONS DOCUMENTS

    SciTech Connect

    BERGLIN, E J

    2003-06-23

    This report provides the technical basis for high efficiency particulate air filter (HEPA) for Hanford tank farm ventilation systems (sometimes known as heating, ventilation and air conditioning [HVAC]) to support limits defined in Process Engineering Operating Specification Documents (OSDs). This technical basis included a review of older technical basis and provides clarifications, as necessary, to technical basis limit revisions or justification. This document provides an updated technical basis for tank farm ventilation systems related to Operation Specification Documents (OSDs) for double-shell tanks (DSTs), single-shell tanks (SSTs), double-contained receiver tanks (DCRTs), catch tanks, and various other miscellaneous facilities.

  13. Software Requirements Specification: Multi-scale Epidemiological and Economic Simulation and Analysis (MESA) Scenario Bank

    SciTech Connect

    Dahlgren, T L; Hazlett, S G; Slone, D M; Smith, S G

    2006-11-08

    This document builds on the discussion notes from September 21, 2006. It provides a summary of the ideas relating to the scenario bank tables and their associated requirements. Two conceptual groupings were identified for the contents requirements of the scenario bank. The first, called ProjectTemplate, shall consist of groups. The second, ProjectArchive, shall consist of groups of . The figure below illustrates the multiplicity of the associations between the different tables, with color coding used to distinguish between current MESA (brown) and USDA (light green) requirements. Scenario bank tables are shown in black with their general contents specified within the box. The metadata associated with each table is expected to include database key information as well as relevant timestamps. Each File is expected to be a file with an arbitrary format.

  14. APPLICATION OF NONSPHERICAL FISSILE CONFIGURATION IN WASTE CONTAINERS AT SRS

    SciTech Connect

    Eghbali, D; Michelle Abney, M

    2007-01-03

    Transuranic (TRU) solid waste that has been generated as a result of the production of nuclear material for the United States defense program at the Savannah River Site (SRS) has been stored in more than 30,000 55-gallon drums and carbon steel boxes since 1953. Nearly two thirds of those containers have been processed and shipped to the Waste Isolation Pilot Plant. Among the containers assayed so far, the results indicate several drums with fissile inventories significantly higher (600-1000 fissile grams equivalent (FGE) {sup 239}Pu) than their original assigned values. While part of this discrepancy can be attributed to the past limited assay capabilities, human errors are believed to be the primary contributor. This paper summarizes the application of nonspherical fissile material configuration in waste containers, resulting in less restrictive mass and spacing limits, increased storage capacity, and several administrative controls for handling and storage of waste containers being modified without compromising safety.

  15. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  16. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  17. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  18. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  19. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  20. Standard specification for general requirements for steel plates for pressure vessels. ASTM standard

    SciTech Connect

    1998-11-01

    This specification is under the jurisdiction of ASTM Committee A-1 on Steel, Stainless Steel, and Related Alloys and is the direct responsibility of Subcommittee A01.11 on Steel Plates for Boilers and Pressure Vessels. Current edition approved Sep. 10, 1997. Published November 1998. Originally published as A 20-50T. Last previous edition A 20/A 20M-97a.

  1. 78 FR 30245 - Electric Reliability Organization Interpretation of Specific Requirements of the Disturbance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... Environmental Policy Act, Order No. 486, 52 FR 47897 (Dec. 17, 1987), FERC Stats. & Regs. Regulations Preambles... Energy Regulatory Commission 18 CFR Part 40 Electric Reliability Organization Interpretation of Specific... for approval by the North American Electric Reliability Corporation, the Commission-certified...

  2. NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae

    SciTech Connect

    Xu, Liuzhong; Ajimura, M.; Padmore, R.; Klein, C.; Kleckner, N.

    1995-12-01

    This report describes the identification of a new meiosis-specific gene of Saccharomyces cerevisiae called NDT80. DNA cloning and molecular analysis revealed that the NDT80 gene maps on the right arm of chromosome 8 and is transcribed during middle meiotic prophase. 82 refs., 6 figs., 3 tabs.

  3. 24 CFR 200.955 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... incorporation by reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51, and is available from the... (AAMA) standard, AAMA/NWWDA 101/I.S.2-97 Voluntary Specifications for Aluminum, Vinyl (PVC) and...

  4. 49 CFR 180.205 - General requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... otherwise represent that a DOT specification or special permit cylinder has been requalified unless all... special permit number must be requalified and marked in conformance with this section and the terms of the applicable special permit. No cylinder may be filled with a hazardous material and offered for...

  5. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  6. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  7. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... a FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as...

  8. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  9. Experimental Determination and Thermodynamic Modeling of Electrical Conductivity of SRS Waste Tank Supernate

    SciTech Connect

    Pike, J.; Reboul, S.

    2015-06-01

    SRS High Level Waste Tank Farm personnel rely on conductivity probes for detection of incipient overflow conditions in waste tanks. Minimal information is available concerning the sensitivity that must be achieved such that that liquid detection is assured. Overly sensitive electronics results in numerous nuisance alarms for these safety-related instruments. In order to determine the minimum sensitivity required of the probe, Tank Farm Engineering personnel need adequate conductivity data to improve the existing designs. Little or no measurements of liquid waste conductivity exist; however, the liquid phase of the waste consists of inorganic electrolytes for which the conductivity may be calculated. Savannah River Remediation (SRR) Tank Farm Facility Engineering requested SRNL to determine the conductivity of the supernate resident in SRS waste Tank 40 experimentally as well as computationally. In addition, SRNL was requested to develop a correlation, if possible, that would be generally applicable to liquid waste resident in SRS waste tanks. A waste sample from Tank 40 was analyzed for composition and electrical conductivity as shown in Table 4-6, Table 4-7, and Table 4-9. The conductivity for undiluted Tank 40 sample was 0.087 S/cm. The accuracy of OLI Analyzer™ was determined using available literature data. Overall, 95% of computed estimates of electrical conductivity are within ±15% of literature values for component concentrations from 0 to 15 M and temperatures from 0 to 125 °C. Though the computational results are generally in good agreement with the measured data, a small portion of literature data deviates as much as ±76%. A simplified model was created that can be used readily to estimate electrical conductivity of waste solution in computer spreadsheets. The variability of this simplified approach deviates up to 140% from measured values. Generally, this model can be applied to estimate the conductivity within a factor of two. The comparison of the

  10. Colloid and ionic tracer migration within SRS sediments: Final summary

    SciTech Connect

    Strom, R.N.; Seaman, J.C.; Bertsch, P.M.; Miller, W.P.

    1996-04-09

    The generation of a stable colloidal suspension in geologic materials has a number of environmental implications. Mobile colloids may act as vectors for the transport of adsorbed contaminants through soils and within aquifers and can cause serious problems related to well monitoring and formation permeability in an injections well system. Colloid-facilitated transport has been implicated in the migration of contaminants from seepage basins on the Department of Energy`s Savannah River Site (SRS) at a rate greater than was predicted in two- phase transport models. From 1955 to 1988, seepage basins overlying the water-table aquifer received acidic wastes containing high levels of Na+ and nitric acid, as well as trace radionuclides and metals from the nuclear materials processing facilities. Numerical simulations predicted that metal contaminants would not reach the water table, but measurable quantities of these contaminants have been detected in monitoring wells down gradient from the basins. Lack of agreement between predicted and observed contaminant migration in this and other studies has been attributed to both local non equilibrium situation, preferential flow paths within the geologic material, and to transport of the contaminant in association with a mobile solid phase, i.e. dispersed colloids. Additionally, the association of contaminants with a mobile colloidal phase has important ramifications for groundwater sampling on SRS intended to evaluate the potential environmental hazards of a given contaminant. As part of the F- and H-Area reclamation project, the Department of Energy has proposed the capture and treatment of the contaminant plume followed by reinjection of the treated water into the water table and upper confined aquifers. (Abstract Truncated)

  11. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CFR 73.59 are exempt from the criminal history records check and background check requirements in... system not easily discernible by members of the public; (iii) Alarm system layouts showing the location of intrusion detection devices, alarm assessment equipment, alarm system wiring, emergency...

  12. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... CFR 73.59 are exempt from the criminal history records check and background check requirements in... system not easily discernible by members of the public; (iii) Alarm system layouts showing the location of intrusion detection devices, alarm assessment equipment, alarm system wiring, emergency...

  13. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... CFR 73.59 are exempt from the criminal history records check and background check requirements in... system not easily discernible by members of the public; (iii) Alarm system layouts showing the location of intrusion detection devices, alarm assessment equipment, alarm system wiring, emergency...

  14. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CFR 73.59 are exempt from the criminal history records check and background check requirements in... system not easily discernible by members of the public; (iii) Alarm system layouts showing the location of intrusion detection devices, alarm assessment equipment, alarm system wiring, emergency...

  15. 33 CFR 155.5035 - Nontank vessel response plan requirements: Specific content.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... pollution incident as required 33 CFR 151.26(b)(3)(iii). This information must also clearly specify who will.../78 (as set forth in 33 CFR 151.26 and 33 CFR part 153); and (ii) Any applicable State; (2....” See 33 CFR 155.5020, paragraph (5) of the definition of “Contract or other approved means.” (3)...

  16. 40 CFR 270.18 - Specific part B information requirements for waste piles.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... complied with; (h) A description of how hazardous waste residues and contaminated materials will be removed... requirements for waste piles. 270.18 Section 270.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) EPA ADMINISTERED PERMIT PROGRAMS: THE HAZARDOUS WASTE PERMIT...

  17. Activation-specific metabolic requirements for NK cell IFN-γ production1

    PubMed Central

    Keppel, Molly P.; Topcagic, Nermina; Mah, Annelise Y.; Vogel, Tiphanie P.; Cooper, Megan A.

    2014-01-01

    There has been increasing recognition of the importance of cellular metabolism and metabolic substrates for the function and differentiation of immune cells. Here, for the first time, we investigate the metabolic requirements for production of IFN-γ by freshly isolated NK cells. Primary murine NK cells mainly utilize mitochondrial oxidative phosphorylation at rest and with short-term activation. Remarkably, we discovered significant differences in the metabolic requirements of murine NK cell IFN-γ production depending upon the activation signal. Stimulation of NK cell IFN-γ production was independent of glycolysis or mitochondrial oxidative phosphorylation when cells were activated with IL-12+IL-18. By contrast, stimulation via activating NK receptors required glucose-driven oxidative phosphorylation. Prolonged treatment with high-dose, but not low dose, IL-15 eliminated the metabolic requirement for receptor stimulation. In summary, this study demonstrates that metabolism provides an essential second signal for induction of IFN-γ production by activating NK cell receptors that can be reversed with prolonged high-dose IL-15 treatment. PMID:25595780

  18. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Drains Penetrating Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The...) Subdivision and stability. Plans and calculations as required by Subchapter S of this chapter. (d) Fire.... (2) Ventilation diagram including dampers and other fire control features. (3) Details of...

  19. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Drains Penetrating Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The...) Subdivision and stability. Plans and calculations as required by Subchapter S of this chapter. (d) Fire.... (2) Ventilation diagram including dampers and other fire control features. (3) Details of...

  20. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Drains Penetrating Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The...) Subdivision and stability. Plans and calculations as required by Subchapter S of this chapter. (d) Fire.... (2) Ventilation diagram including dampers and other fire control features. (3) Details of...

  1. 40 CFR 60.2660 - What site-specific documentation is required?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... recordkeeping procedures. (8) The waste management plan required under §§ 60.2620 through 60.2630. (9... Compliance Times for Commercial and Industrial Solid Waste Incineration Units that Commenced Construction On or Before November 30, 1999 Model Rule-Operator Training and Qualification § 60.2660 What...

  2. 40 CFR 270.19 - Specific part B information requirements for incinerators.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... burned including: (i) Heat value of the waste in the form and composition in which it will be burned. (ii... (CONTINUED) SOLID WASTES (CONTINUED) EPA ADMINISTERED PERMIT PROGRAMS: THE HAZARDOUS WASTE PERMIT PROGRAM... incinerate hazardous waste must fulfill the requirements of paragraphs (a), (b), or (c) of this section....

  3. 40 CFR 270.18 - Specific part B information requirements for waste piles.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... complied with or detailed plans and an engineering report describing how the requirements of § 264.90(b)(2) will be met. (c) Detailed plans and an engineering report describing how the waste pile is designed and...(b) of this chapter, submit detailed plans, and engineering and hydrogeological reports,...

  4. 40 CFR 270.18 - Specific part B information requirements for waste piles.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... complied with or detailed plans and an engineering report describing how the requirements of § 264.90(b)(2) will be met. (c) Detailed plans and an engineering report describing how the waste pile is designed and...(b) of this chapter, submit detailed plans, and engineering and hydrogeological reports,...

  5. 40 CFR 270.24 - Specific part B information requirements for process vents.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... cannot install a closed-vent system and control device to comply with the provisions of 40 CFR 264 subpart AA on the effective date that the facility becomes subject to the provisions of 40 CFR 264 or 265... requirements for process vents. 270.24 Section 270.24 Protection of Environment ENVIRONMENTAL PROTECTION...

  6. 40 CFR 270.25 - Specific part B information requirements for equipment.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... closed-vent system and control device to comply with the provisions of 40 CFR 264 subpart BB on the effective date that the facility becomes subject to the provisions of 40 CFR 264 or 265 subpart BB, an... requirements for equipment. 270.25 Section 270.25 Protection of Environment ENVIRONMENTAL PROTECTION...

  7. 40 CFR 270.25 - Specific part B information requirements for equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... closed-vent system and control device to comply with the provisions of 40 CFR 264 subpart BB on the effective date that the facility becomes subject to the provisions of 40 CFR 264 or 265 subpart BB, an... requirements for equipment. 270.25 Section 270.25 Protection of Environment ENVIRONMENTAL PROTECTION...

  8. 40 CFR 270.25 - Specific part B information requirements for equipment.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... closed-vent system and control device to comply with the provisions of 40 CFR 264 subpart BB on the effective date that the facility becomes subject to the provisions of 40 CFR 264 or 265 subpart BB, an... requirements for equipment. 270.25 Section 270.25 Protection of Environment ENVIRONMENTAL PROTECTION...

  9. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... oxygen service conform to the requirements of this paragraph, except when the cylinder is used for...-belt pretensioners meeting the criteria for a Division 1.4G material. (3) Chemical oxygen generators. Chemical oxygen generators must be approved, classed, described, packaged, and transported in...

  10. Research in Workplace Literacy: The Level of Literacy Required in Specific Occupations. Final Report.

    ERIC Educational Resources Information Center

    Grover, Jane G.; And Others

    A study identified trends influencing the literacy requirements of two entry-level jobs at each of three manufacturing sites and three hospital sites. Data were collected through observation, interviews, and focus groups with workers, employers, and union representatives. Materials that workers were expected to read were analyzed. The study…

  11. Host specific differences alter the requirement for certain Salmonella genes during swine colonization

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The pathogenic potential of Salmonella is determined during the complex interaction between pathogen and host, requiring optimal regulation of multiple bacterial genetic systems within variable in vivo environments. The mouse model of systemic disease has been an extremely productive model to invest...

  12. Tissue-specific requirements of beta-catenin in external genitalia development.

    PubMed

    Lin, Congxing; Yin, Yan; Long, Fanxin; Ma, Liang

    2008-08-01

    External genitalia are body appendages specialized for internal fertilization. Their development can be divided into two phases, an early androgen-independent phase and a late androgen-dependent sexual differentiation phase. In the early phase, the embryonic anlage of external genitalia, the genital tubercle (GT), is morphologically identical in both sexes. Although congenital external genitalia malformations represent the second most common birth defect in humans, the genetic pathways governing early external genitalia development and urethra formation are poorly understood. Proper development of the GT requires coordinated outgrowth of the mesodermally derived mesenchyme and extension of the endodermal urethra within an ectodermal epithelial capsule. Here, we demonstrate that beta-catenin plays indispensable and distinct roles in each of the aforementioned three tissue layers in early androgen-independent GT development. WNT-beta-catenin signaling is required in the endodermal urethra to activate and maintain Fgf8 expression and direct GT outgrowth, as well as to maintain homeostasis of the urethra. Moreover, beta-catenin is required in the mesenchyme to promote cell proliferation. By contrast, beta-catenin is required in the ectoderm to maintain tissue integrity, possibly through cell-cell adhesion during GT outgrowth. The fact that both endodermal and ectodermal beta-catenin knockout animals develop severe hypospadias in both sexes raises the possibility that the deregulation of any of these functions can contribute to the etiology of congenital external genital defects in humans. PMID:18635608

  13. 49 CFR 173.472 - Requirements for exporting DOT Specification Type B and fissile packages.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of the quality assurance program required by 10 CFR part 71, subpart H, or 49 CFR 173.474 and 173.475... CFR part 71, subpart H, or 49 CFR 173.474 and 173.475, and a reproducible 22 cm × 30 cm (8.5″×11... received at least 90 days before the requested effective date....

  14. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., or a DOT exemption or special permit cylinder or a cylinder used as a fire extinguisher in... oxygen service conform to the requirements of this paragraph, except when the cylinder is used for... relief devices and provide appropriate guidance for exposure to fire. (b) Conditions and...

  15. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., or a DOT exemption or special permit cylinder or a cylinder used as a fire extinguisher in... oxygen service conform to the requirements of this paragraph, except when the cylinder is used for... relief devices and provide appropriate guidance for exposure to fire. (b) Conditions and...

  16. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., or a DOT exemption or special permit cylinder or a cylinder used as a fire extinguisher in... oxygen service conform to the requirements of this paragraph, except when the cylinder is used for... receptacles are not fitted with pressure relief devices and provide appropriate guidance for exposure to...

  17. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., or a DOT exemption or special permit cylinder or a cylinder used as a fire extinguisher in... oxygen service conform to the requirements of this paragraph, except when the cylinder is used for... relief devices and provide appropriate guidance for exposure to fire. (b) Conditions and...

  18. Specification of requirements for the virtual environment for reactor applications simulation environment

    SciTech Connect

    Hess, S. M.; Pytel, M.

    2012-07-01

    In 2010, the United States Dept. of Energy initiated a research and development effort to develop modern modeling and simulation methods that could utilize high performance computing capabilities to address issues important to nuclear power plant operation, safety and sustainability. To respond to this need, a consortium of national laboratories, academic institutions and industry partners (the Consortium for Advanced Simulation of Light Water Reactors - CASL) was formed to develop an integrated Virtual Environment for Reactor Applications (VERA) modeling and simulation capability. A critical element for the success of the CASL research and development effort was the development of an integrated set of overarching requirements that provides guidance in the planning, development, and management of the VERA modeling and simulation software. These requirements also provide a mechanism from which the needs of a broad array of external CASL stakeholders (e.g. reactor / fuel vendors, plant owner / operators, regulatory personnel, etc.) can be identified and integrated into the VERA development plans. This paper presents an overview of the initial set of requirements contained within the VERA Requirements Document (VRD) that currently is being used to govern development of the VERA software within the CASL program. The complex interdisciplinary nature of these requirements together with a multi-physics coupling approach to realize a core simulator capability pose a challenge to how the VRD should be derived and subsequently revised to accommodate the needs of different stakeholders. Thus, the VRD is viewed as an evolving document that will be updated periodically to reflect the changing needs of identified CASL stakeholders and lessons learned during the progress of the CASL modeling and simulation program. (authors)

  19. 10 CFR 110.32 - Information required in an application for a specific license/NRC Form 7.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Information required in an application for a specific license/NRC Form 7. 110.32 Section 110.32 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) EXPORT AND...) and its chemical and physical form. (2) For enriched uranium, the maximum weight percentage...

  20. Hemogenic endothelial cell specification requires c-kit, notch signaling, and p27-mediated cell-cycle control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Delineating the mechanism or mechanisms that regulate the specification of hemogenic endothelial cells from primordial endothelium is critical for optimizing their derivation from human stem cells for clinical therapies. We previously determined that retinoic acid (RA) is required for hemogenic spec...