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Estagio atual de conhecimento da geoquimica organica das bacias de Campos e Espirito Santo. (Actual stage of organic geochemical knowledge from Campos and Espirito Santo basins, Brazil).  

National Technical Information Service (NTIS)

Campos and Espirito Santo basins display several similar geochemical aspects. The microbial and other degradation processes caused changes in the composition of the oils in the reservoir rocks. The oils are biodegraded in different degrees, with the reser...

P. C. Gaglianone L. A. F. Trindade



Vin Santo.  


Vin Santo is the typical dessert wine produced in Tuscany, in particular, and in other areas of central and northern Italy, as well as on the Greek island of Santorini. It remains one of the most important "meditation wines", whose glamour starts with its ancient and mysterious origin. Italy is the country with the World's greatest tradition for sweet wine production. In this contest most of the steps in the Vin Santo making process are still linked to old local traditions. This chapter provides an overview of the different styles of Vin Santo produced in Italy together with its marketing. The main factors affecting grapes drying, alcoholic fermentation, maturation in barrels, are also described. So far there have been few scientific studies that have focused on Vin Santo. However, the increasing demand registered in the last few years for this kind of wine, is now greatly stimulating the research aimed to better manage its peculiar production process. PMID:21867892

Domizio, Paola; Lencioni, Livio




Microsoft Academic Search

As Reservas Extrativistas (RESEX) são áreas de domínio público, com uso concedido às populações extrativistas tradicionais, cuja subsistência baseia-se no extrativismo e na agricultura de subsistência e na criação de animais de pequeno porte, e têm como objetivos básicos proteger os meios de vida e a cultura dessas populações, e assegurar o uso sustentável dos recursos naturais da unidade. Porém,

Francisco Carlos Da Silveira Cavalcanti; Raimundo Claudio Gomes Maciel; Joao Alfredo De Carvalho Mangabeira; Bastiaan Philip Reydon



Metáforas de Lula e Alckmin nos debates de 2006 em uma perspectiva da Lingüística de Corpus1  

Microsoft Academic Search

RESUMO: O objetivo deste trabalho é contrastar o uso de metáforas pelos dois candidatos no segundo turno das eleições presidenciais de 2006, Luís Inácio Lula da Silva e Geraldo Alckmin, para saber até que ponto Lula, que foi vitorioso nas urnas, usou metáforas de modo mais eficaz do que o outro candidato durante os debates televisados. Foi colhido um corpus

Tony Berber Sardinha


[Haitian migration to Santo Domingo].  


This work examines the history of Haitian migration to the Dominican Republic, the central role of Haitian migration in Dominican society, working conditions of Haitian migrants in the Dominican Republic, and the relationship of the migration to economic development on the island of Hispaniola. Lack of data, the difficulty of measuring illegal movement, and the problem of defining Haitians in Santo Domingo have impeded understanding of migration to the Dominican Republic. It is believed by many authorities that Haitian migration to Santo Domingo is considerable and perhaps exceeds that to the US. Haitian migration to the Dominican Republic began after 1915 with the fall of the Haitian president, a worsening of economic conditions partly caused by stagnation in the agricultural sector, and the newly dominant role of the US in Haitian economic affairs. The Great Depression of the 1930s was a direct antecedent of the massacre of Haitians by Dominican police in which some 30 thousand persons were killed; the economic recession of the early 1980s has also caused an outburst of antiHaitian feeling in the Dominican Republic although 80% of laborers in the sugar industry are Haitians. Sugar is extremely important to the Dominican economy: in 1974, sugar covered 12% of cultivated land, produced 40% of foreign exchange earnings, and was responsable for 21% of taxable income. Dominicans however refuse to work in sugar plantations under the current technological. conditions and wage system. Although the government periodically demands the Dominicanization of the sugar work force, no such changes have been made. Sugar will probably continue to play a decisive role in the generation of foreign exchange despite introduction of more technologically advanced sectors which benefit from better prices in the international market. Possibilities of mechanizing sugar production in the Dominican Republic appear remote, and failure to modernize an important sector of the economy has explained migration of unskilled labor from poorer countries in more than 1 case. Under such circumstances, the extremely poor working conditions of Haitians in the Dominican Republic are not surprising. A 1983 investigation by the International Labour Organization indicated that salaries were low, that a large proportion was routinely diverted from the migrants, that hours of work were long with no regular rest and few days off, that few workers had any contractual protection, that forced labor recruitment occurred, that a rigid system of vigilance with armed patrols was used to keep the immigrants in the work camps, and that living conditions were substandard--in short, that Haitian workers were "neo-slaves". In addition, the Dominicans consider themselves racially and culturally superior to Haitians. The Haitian government, in return for payments from the Dominican Republic, does nothing to stop the abuses. PMID:12179414

Latortue, P R



Carbon and Oxygen Stable Isotope Data as Paleoenvironmental Indicators for Limestones from the Campos, Santos and Espirito Santo Basins, Brazil.  

National Technical Information Service (NTIS)

Carbon and oxygen isotope data of limestones from Campos, Santos and Espirito Santo basins provided additional information on the sedimentation environments of these carbonates. The predominance of gamma sup 13 C values between + 1.0 per mille and - 10 pe...

T. Takaki R. Rodrigues



Ostracodes from the Aptian-Santonian of the Santos, Campos and Espírito Santo basins, Brazil  

NASA Astrophysics Data System (ADS)

The Lower and Upper Cretaceous deposits from the Brazilian marginal basins present a rich and diversified fauna of ostracodes in continental, marine and mixohaline paleoenvironments. While the Cretaceous ostracodes from the northeastern region have already been the subject of many taxonomic and biostratigraphical studies, data from the southeastern basins are still scarce and restricted to the record of a few species. The present study represents a comprehensive contribution to the systematic knowledge of the Aptian-Santonian ostracodes from the Brazilian southeastern marginal basins. A total of 1045 cutting samples were analyzed from 10 wells drilled offshore in Santos, Campos and Espírito Santo basins. Thirty-eight ostracode taxa were recorded from different depositional environments, including the following six new species: Amphicytherura fragilis sp. nov., Brachycythere multidifferentis sp. nov., Fossocytheridea ballentae sp. nov., Fossocytheridea elegans sp. nov., Nigeroloxoconcha itanhaensis sp. nov. and Paracypris eniotmetos sp. nov.

Piovesan, Enelise Katia; Nicolaidis, Demétrio Dias; Fauth, Gerson; Viviers, Marta Claudia



The ethnopharmacology of Madeira and Porto Santo Islands, a review  

Microsoft Academic Search

The ethnopharmacology of Madeira and Porto Santo Islands is extremely interesting because of the cultural and biogeographic features of this region, which make it a centre of medicinal plant diversity (richness of endemic flora, geographical isolation in the Atlantic sea, crosscultural influences, and past abundance of local healers). The medicinal flora of Madeira is composed of 259 species. Some noteworthy

D. Rivera; C. Obón



Continuous Mantle Exhumation at the Outer Continental Margin of the Santos, Campos and Espírito Santo Basins, Brazil  

NASA Astrophysics Data System (ADS)

The interpretation of 12,000 km of very deep (PSTM to 16 sec., PSDM to 25 km) 2D seismic sections, coupled with gravimetric and magnetometric modeling line-by-line, and the integration of the results with the regional data bank of Petrobras, all together viewed in terms of the recent tectonic models developed for the rupturing and separation of mega-plates, led to a regional (500,000 km2), first-time ever, 3D-view of the deep structure underlying the prolific sedimentary basins of Santos, Campos and Espírito Santo in southeastern Brazil. The three basins are situated onto a continental margin that narrows gradually, from south to north, from a very wide (Santos), through an intermediate (Campos), and then to a narrow (Espírito Santo) passive margin. The seismic sections shows very well the dual rheological behavior of the continental crust, consisting of a deeper and plastic lower crust (with numerous short and strong reflections that display sub-horizontal ductile flow) and a shallower and brittle upper crust (represented by a mostly transparent and faulted seismic facies topped by the sedimentary sections of the rift and thermal subsidence phases). The crustal structure of the Santos Basin shows a zonation from west to east of alternating bands of NE-SW-trending thin (plastic basement terrains) and thick (resistant basement terrains) stretched continental crust. In vertical section this zonation is displayed as a series of necking zones, leading to a highly irregular, low to moderate crustal taper. Such zonation is less developed in the Campos Basin, where the crustal taper is moderate and regular, and practically non-existent in the Espírito Santo Basin, where the crustal taper is high. The most outstanding crustal feature shared in common by the three basins is the exhumation of mantle between the tip of the hyper-extended continental crust and the tabular-shaped oceanic crust. Although the crustal taper varies significantly from basin to basin their continental crust pinches out invariably on the flanks of exhumed mantle. This gives rise to a remarkable long (900 km along a N-S direction and 600 km in E-W direction), relatively narrow (15 to 70 km wide) and continuous belt of exhumed mantle that marks the passage from continental crust to oceanic crust in all three basins. The Santos, Campos and Espírito Santo Basins thus form a typical magma-poor passive margin. These are in sharp contrast with the adjacent basin to the south, the Pelotas Basin, that in turn is a typical volcanic passive margin displaying a long (1000 km in a N-S direction) and wide (100 to 220 km) belt of seaward-dipping reflectors at its outer margin and no exhumation of the mantle at the continent-ocean boundary.

Zalan, P. V.; Severino, M. G.; Rigoti, C. A.; Magnavita, L. P.; Oliveira, J. B.; Viana, A. R.




Microsoft Academic Search

SUMMARY Blue marlin (Makaira nigricans), white marlin (Tetrapturus albidus), and sailfish (Istiophorus platypterus) are caught off the south and southeast Brazilian coasts by Brazilian and leased (Hon- duras and Barbados flags) longliners of Santos city, São Paulo State, Brazil. This paper reports statistics for the Santos fleet including blue marlin, white marlin and sailfish annual yield for 1971-99, monthly yield

A. F. Amorim; C. A. Arfelli


The Role of Rhetoric in the Judicial Opinion: The Rhetoric of Boaventura De Sousa Santos.  

ERIC Educational Resources Information Center

Boaventura De Sousa Santos, a Portuguese law professor, appears to be the first legal scholar and theorist who works with rhetorical theories and who is accepted by the legal profession. Santos's major works have centered on the legal system of Pasargada, a Brazilian favela (squatter village) whose residents try to settle disputes outside the…

Anapol, Malthon M.



Microsoft Academic Search

RESUMO: O estudo foi baseado no projeto da OEA e adaptado pela Escola de Enfermagem da UNIFESP, buscando analisar a situação sobre os conteúdos de álcool e outras drogas ministradas na graduação de enfermagem no Brasil. O objetivo desse trabalho foi avaliar a situação do ensino sobre a temática, álcool e drogas, na graduação em Enfermagem da Universidade Federal do

Robison Boni; Sandra C Pillon; Elisangela C. Santos; Marcio W. Camata; Marluce S. Macieira


Evolution of tricyclic alkanes in the Espirito Santo Basin, Brazil  

NASA Astrophysics Data System (ADS)

The distributions of tricyclic isoprenoid alkanes were characterized in the Neocomian sequence from the Espirito Santo Basin, Brazil, by high-resolution gas chromatography-mass spectrometry and co-injection with synthetic standards where available. A suite of homologous components ranging from C 20 to C 28 were present throughout the sequence as a mixture of stereoisomers whose relative abundance changed with depth. The epimerization rates were similar for the various tricyclic compounds irrespective of side-chain length, achieving equilibrium ratios before the main stage of oil generation. This new maturity parameter should be useful as a supplement to sterane/hopane measurements.

Aquino Neto, F. R.; Cardoso, J. N.; Rodrigues, R.; Trindade, L. A. F.



Bathymetry, Chirp and Deep Crustal Structure of the Santos Basin SÃO Paulo Ridge Complex (sbspr)  

NASA Astrophysics Data System (ADS)

The SanBa (Santos Basin- Seismic Research experiment) research experiment is a joint project of the Department of Marine Geosciences (IFREMER: Institut Français de Recherche pour l'Exploitation de la MER, France), the Laboratory of "Oceanic Domain" (Institut Universitaire et Européen de la Mer, France), the Faculdade de Ciências da Universidade de Lisboa (Lattex and CGUL, Portugal), the Universidade de Brasilia (Brazil) and PETROBRAS. Its aim is to test hypotheses that have been proposed such as the existence of failed rift and a micro-block (Moulin et al., GSL submitted) or the presence of exhumed mantle on its south-eastern part (Zalan et al., AAPG 2009). Six wide-angle seismic data were acquired together with coincident deep frequency reflection seismic data during the SanBa cruise in Dec 2010 - Jan. 2011 (total > 850 Nm). Chirp and Bathymetry were also acquired during the cruise. The preliminary results suggest a very thin crust (< 5km) in the center and in the south-eastern part of the SBSPR. Both refraction and reflection data present a clear signal of the Moho in the distalmost part of the study area, which seems to preclude the exhumed mantle hypothesis."

Aslanian, D.; Klingelhoefer, F.; Moulin, M.; Schnurle, P.; Rabineau, M.; Afilhado, A.; Roest, W. R.; Feld, A.; Evain, M.; Rochat, A.; Rousic, D.; Rigoti, C. A.; Capechi, E.; Bochenek, G.; Viana, A. R.; Magnavita, L. P.; Szatmari, P.; Neto, M.; Soares, J. P.; Fuck, R. A.; Paula Ribas, M.; De Lima, M.; Corela, C.; Duarte, J.; Matias, L. M.; OBS Team of Sanba Cruise



The ethnopharmacology of Madeira and Porto Santo Islands, a review.  


The ethnopharmacology of Madeira and Porto Santo Islands is extremely interesting because of the cultural and biogeographic features of this region, which make it a centre of medicinal plant diversity (richness of endemic flora, geographical isolation in the Atlantic sea, crosscultural influences, and past abundance of local healers). The medicinal flora of Madeira is composed of 259 species. Some noteworthy medicinal taxa, endemic or locally relevant, are: Acanthus mollis, Aeonium glandulosum, Aeonium glutinosum, Bidens pilosa, Borago officinalis, Chamaemelum nobile var. discoideum, Culcita macrocarpa, Echium nervosum, Euphorbia platiphylla, Helichrysum melaleucum, Helichrysum obconicum, Hypericum glandulosum, Hypericum humifussum, Kleinia repens, Laurus azorica, Monizia edulis, Ocotea foetens, Psoralea bituminosa, Rubus bollei, Rumex maderensis, Sambucus lanceolata, Scilla maderensis, Sedum brissemoretii, Sedum farinosum, Sedum nudum, Sibthorpia peregrina, Teucrium betonicum, Thymus caespititius, Trifolium squamosum and Vaccinium padifolium. Among the medicinal cryptogams, one can underline the parasitic fungus Laurobasidium lauri, which grows on the stems of Laurus azorica and is used as an antirheumatic, haemostatic, emmenagogue, insecticide and analeptic. PMID:7650952

Rivera, D; Obón, C



HIV infection and risk behaviors among male port workers in Santos, Brazil.  

PubMed Central

OBJECTIVES. This paper measured the extent to which human immunodeficiency virus (HIV) infection has spread among the male working-class population of Santos, Brazil. METHODS. Questionnaires on risk behaviors and blood tests were administered to a random sample (n = 395) of male port workers employed by the Santos Port Authority. RESULTS. Although the rate of HIV infection among these men- the working-class male population of Santos-remains low (1.1%), self-reported behavioral risks for HIV infection are common. CONCLUSIONS. There is still time to prevent a widespread outbreak of HIV infection among the hetero-sexual population of Santos and of the transportation corridors emanating from that city.

Larcerda, R; Stall, R; Gravato, N; Tellini, R; Hudes, E S; Hearst, N



Aliphatic and Aromatic Hydrocarbons in Sediments of Santos and Cananéia, SP, Brazil  

Microsoft Academic Search

Sediment samples from Santos and Cananéia, São Paulo Brazil were analysed by GC–FID and GC–MS for aliphatic and aromatic hydrocarbons in order to gather information on the degree of contamination by oil and other biogenic contributions. Concentrations of total n-alkanes in Santos varied from 1.05 to 4.29?gg?1 and aromatic hydrocarbons from 0.08 to 42.39?gg?1. In Cananéia total n-alkanes varied from

Fernando Noboru Nishigima; Rolf Roland Weber; Márcia Caruso B??cego



Palaeomagnetic Investigations on Santo Antao, Cape Verde Islands  

NASA Astrophysics Data System (ADS)

Samples from five hitherto fairly unexplored young volcanic islands in the Cape Verde Archipelago have been collected for palaeomagnetic studies. Magnetic results from lava flows in 5 profiles located on the island Santo Antão are reported. The Cape Verde Rise situated over the Mesozoic marine anomalies M2-M16, is the result of a slow eastward movement of the Atlantic oceanic lithosphere relative to a hotspot commencing around 20 Ma ago. The uplifted segment of deformed Jurassic oceanic crust seems to be unrelated to the bulk of the igneous activity, which took place during the Neogene. The oldest outcrops encountered on Santo Antão are deeply weathered basalts with an age of 7.57 +/- 0.56 Ma, whereas the investigated profiles cover a time interval from 2.67 +/- 0.05 Ma to 0.38 +/- 0.05 Ma. Investigation of some basic mineral magnetic properties reveal titanomagnetite composition as a general feature, and a pseudo-single-domain behaviour of all lava flows subjected to hysteresis-experiments are found. Flows of normal polarity characterise the Tarrafal Profile, being confined to the Brunhes Chron by 40Ar/39Ar-datings. Two events of reverse polarity are recorded in the stratigraphic column and appear to represent the Big Lost and Emperor Subchrons. The minimum eruption frequency is estimated to be one flow every 2.5 +/- 2.2 Ka. The Agua Nova Profile is likewise dominated by flows of normal polarity belonging to the Brunhes Chron and further contains a few events of reverse polarity. The relatively complex stratigraphy of accessible flows encountered in the Ribeira Grande Area makes the correlation with the global polarity time-scale tentative. The Escabecada Profile records the transition between the lower part of Matuyama and the upper part of the Gauss Chron, and a minimum eruption frequency is estimated to be one flow every 10 +/- 7.1 Ka for the lower part of this profile. The Chã de Morte Profile consists merely of reverse-polarity lava flows belonging to the Matuyama Chron. A tentative correlation between all 5 profiles has been attempted by the use of both radiometric datings and magnetic polarities. Polarities from the Escabecada and Agua Nova Profiles appear to indicate the existence of short events of fully reversed polarity not registered as subchrons in the global polarity time-scale. The presence of a reversed polarity event within the Brunhes Chron, as recorded by 8 lava flows in the Tarrafal Profile, may suggest that this geomagnetic event is of a more substantial and permanent nature than typically expected for large-amplitude secular variations.

Knudsen, M. F.; Abrahamsen, N.



No evidence for Brunhes age excursions, Santo Antão, Cape Verde  

NASA Astrophysics Data System (ADS)

We present new 40Ar/ 39Ar ages and palaeomagnetic directions from two re-examined sequences of basaltic lava flows on the island of Santo Antão, Cape Verde: Tarrafal and Agua Nova. We find no evidence for the fully-reversed, Brunhes age excursions reported in Knudsen et al., Earth Planet. Sci. Lett., 206, 199-214, 2003. At Tarrafal, complex geology, including repeated gravitational collapses and/or considerable erosion followed by in-filling of subsequent valleys by younger lavas, coupled with an insufficient number of dated reversely and normally magnetised flows, led earlier researchers to suggest a series of Brunhes age excursions. At Agua Nova, the use of single samples to determine palaeodirections resulted in sampling of anomalous reversed directions, creating apparent excursions. We report new palaeomagnetic directions from Agua Nova, based on multiple measurements per flow determined by both alternating field and thermal demagnetisation. All flows of Brunhes age have virtual geomagnetic pole latitudes > 60°. Our findings highlight the need for precise age constraints and a high sampling density of dated flows, with multiple directional results per flow, when using long lava sequences to reconstruct palaeofield behaviour.

Brown, Maxwell C.; Singer, Brad S.; Knudsen, Mads F.; Jicha, Brian R.; Finnes, Evan; Feinberg, Joshua M.



[The symbolic cartography of epidemiological risk: an incursion into the thinking of Boaventura de Sousa Santos].  


Adopting an interdisciplinary approach, the concept of epidemiological risk is critically discussed. The work of Elizabeth Teixeira, who uses the ideas of Boaventura de Sousa Santos, is presented. The proposal for symbolic cartography of epidemiological risk is sketched out, defining analytical scales ranging from exteriority and distancing from the field of science to the proximity of territory and place, conceptualized on the basis of the geography of Milton Santos, in which conceptions of risk are drawn up from the perspective of the everyday routine of social existence. Questions relating to space, territoriality, subjectivity and time give meaning to a cartography of risk which is proposed as a model for epidemiological investigations. PMID:23070378

Sevalho, Gil; Stotz, Eduardo



New stable isotope results from a 173-year coral from Espiritu Santo, Vanuatu  

NASA Astrophysics Data System (ADS)

Long coral records provide valuable information on pentadal-decadal scale climate variability. Previously we have reported on a preliminary analysis of a 173-year record (1806-1978) from the island of Espiritu Santo (Republic of Vanuatu). Although this record contained information in the pentadal-decadal band, low sampling resolution raised questions about the validity of the results. Herein we report new data from the Santo record, in which the sampling resolution has been doubled and the validation database expanded. Variations in coral ?18O at Santo record the combined effects of variations in SST and rainfall-induced salinity changes. The most prominent spectral peaks in the time series are at the annual cycle and 14-15 and 7.4 years. The robust occurrence of the 14-15 year peak provides additional support to the importance of this oscillation as a cause of decadal-scale climate variability. Overall, our new results provide greater credibility to the conclusions raised in the earlier study and indicate that the Santo record can more confidently be incorporated into the still-small network of multicentury coral records of climate change.

Quinn, Terrence M.; Crowley, Thomas J.; Taylor, Frederick W.


Intoxication in cattle, chicks and hamsters from seed of the palo santo tree (Bulnesia sarmientii).  


A case of intoxication in cattle in Paraguay from seed of the palo santo tree is discussed. Visible toxic signs and lesions observed at necropsy are described. The seed contained 3.5 per cent or more crude saponin. Laboratory studies established that aqueous extracts and crude saponin from the seeds were toxic to one-week-old chicks and adult hamsters. PMID:6523713

Williams, M C; Rodewijk, J C; Olsen, J D


Laurie R. Santos: Award for Distinguished Scientific Early Career Contributions to Psychology  

ERIC Educational Resources Information Center

Presents a short biography of one of the winners of the American Psychological Association's Award for Distinguished Scientific Early Career Contributions to Psychology. The 2012 winner is Laurie R. Santos for creative and insightful investigations of cognition across a broad range of species and psychological domains, illuminating cognitive…

American Psychologist, 2012



Winter limnology of a mountain lake: Lago Santo Parmense (Northern Appennines, Italy)  

Microsoft Academic Search

The evolution of some limnological parameters during the ice cover period was studied in a mountain lake in the Northern Apennines: Lago Santo Parmense (1507 m a.s.l.; maximum depth 22.5 m). The changes in the biomass and production rate of the phytoplankton were analyzed in relation to the changes in the various physical, chemical and biological parameters (the thickness of

I. Ferrari



Intestinal parasites in a quilombola community of the Northern State of Espírito Santo, Brazil.  


The aim of this study was to determine the occurrence of intestinal parasites in a quilombola community from the northern Espírito Santo, Brazil. Descendants of slaves who arrived in Brazil in the sixteenth century, this population settled in the municipality of São Mateus in 1858. Fresh fecal samples from 82 individuals who agreed to participate in the study were collected between August 2009 and July 2010, and immediately sent to the Clinical Laboratory of the Centro Universitário Norte do Espírito Santo of the Universidade Federal do Espírito Santo for analysis. Out of all the participants, 36 (43.9%) were male and 46 (56.1%) were female, whose ages ranged from six to 85 years. The study of the occurrence of intestinal parasites indicated that 35 individuals (42.7%) were infected with at least one intestinal parasite. Among helminths, the most frequent were hookworms, with a rate of 14.6%. With regard to protozoa, Entamoeba coli, Entamoeba histolytica/Entamoeba dispar and Endolimax nana stood out, with frequencies of 23.2%, 8.5% and 4.9%, respectively. The occurrence of biparasitism was observed in 13 of the 82 subjects, accounting for 15.8%, and no cases of multiple parasitic infections were observed. It was concluded that the reduction of cases of intestinal diseases due to parasites will only be achieved with the improvement of basic sanitation and quality of life of quilombola populations. PMID:23740008

Damazio, Schayra Minine; Lima, Marcela de Souza; Soares, Aparecida Rios; Souza, Marco Antônio Andrade de



Outbreak of Hantavirus Pulmonary Syndrome, Los Santos, Panama, 1999-2000  

PubMed Central

An outbreak of hantavirus pulmonary syndrome occurred in the province of Los Santos, Panama, in late 1999 and early 2000. Eleven cases were identified; 9 were confirmed by serology. Three cases were fatal; however, no confirmed case-patient died. Case-neighborhood serologic surveys resulted in an overall hantavirus antibody prevalence of 13% among household and neighborhood members from the outbreak foci. Epidemiologic investigations did not suggest person-to-person transmission of hantavirus infection. By use of Sin Nombre virus antigen, hantavirus antibodies were detected in Oligoryzomys fulvescens and Zygodontomys brevicauda cherriei. This outbreak resulted in the first documented cases of human hantavirus infections in Central America.

Bayard, Vicente; Barria, Eduardo O.; Ruedas, Luis A.; Tinnin, David S.; Munoz, Carlos; de Mosca, Itza B.; Guerrero, Gladys; Kant, Rudick; Garcia, Arsenio; Caceres, Lorenzo; Gracia, Fernando G.; Quiroz, Evelia; de Castillo, Zoila; Armien, Blas; Libel, Marlo; Mills, James N.; Khan, Ali S.; Nichol, Stuart T.; Rollin, Pierre E.; Ksiazek, Thomas G.; Peters, Clarence J.



Cytherellid species (Ostracoda) and their significance to the Late Quaternary events in the Santos Basin, Brazil  

USGS Publications Warehouse

Four autochthonous cytherellid species (Cytherella serratula (BRADY, 1880), C. hermargentina WHATLEY et al. 1998, C. pleistocenica sp. nov. and C. santosensis sp. nov.) have been identified from two offshore cores (44 samples) within the Santos Basin. The distribution of these ostracodes is controlled by local hydrological conditions such as the temperature and, possibly, the oxygen minimum zone (OMZ). However, these factors cannot explain completely the species occurrence in the analysed cores, and relative sea level changes and productivity variation driven by climatic changes are proposed as additional explanations for the faunal distribution pattern. ?? E. Schweizerbart'sche Verlagsbuchhandlung (Na??gele u. Obermiller), 2007.

Bergue, C. T.; Coimbra, J. C.; Cronin, T. M.



Two new species of Blaberidae (Blattaria) collected in the Santa Lúcia Station, Espírito Santo State, Brazil.  


This contribution presents two new species belonging to the genera Audreia (Shelford, 1910) and Epilampra Burmeister, 1838, subfamily Epilamprinae (Blattaria, Blaberidae). The Blaberidae include large and medium-bodied species with a high adaptive capacity. The new taxa were collected in Espírito Santo State and evaluated based on the analysis of subgenital and supranal plates and genital parts, by means of standard dissection techniques, by comparison with specimens of the corresponding genera deposited in the collection of the Museu Nacional and with published descriptions. PMID:24871743

Lopes, Sonia Maria; De Oliveira, Edivar Heeren; Assumpção, Mariana



Distribution of bovine fasciolosis and associated factors in south Espírito Santo, Brazil: an update.  


The geographical distribution and factors associated with bovine fasciolosis in the south of Espírito Santo were updated and the prevalences of this disease and of snails of the genus Lymnaea in the municipality of Jerônimo Monteiro were calculated. In the first stage, fecal samples were collected from 10% of the herds of 115 farms in 23 municipalities and interviews were conducted with owners. Generalized linear mixed models were used. In the second stage, in Jerônimo Monteiro municipality, feces and mollusks were collected from all farms registered in the milk cooperatives in the region. The mollusks were identified and examined for infection by Fasciola hepatica. Fasciolosis was diagnosed in 18 (78%) of the 23 municipalities. Of the 1157 fecal samples examined, 19.01% were positive for eggs of F. hepatica. The final model shows statistical evidence of associations between positive farms and previous cases of fasciolosis and concomitant grazing of cattle with other definitive hosts. In the evaluated farms from the studied municipality the prevalence of fasciolosis and Lymnaea was of 66.7% and 23.8%, respectively. Mollusks were found in flooded areas and the animals' drinking water troughs. The wide geographical distribution of bovine fasciolosis in the south of Espírito Santo requires control measures to prevent its expansion towards the north of this state and other places characterized as F. hepatica free-infection. PMID:24728357

Martins, Isabella Vilhena Freire; Avelar, Barbara Rauta de; Bernardo, Cintia das Chagas; Leão, Alann Casotti de; Salim, Maria Julia



The dynamics of internal tidal waves in Todos Santos Bay, Baja California, during experiment FLOO-2007  

NASA Astrophysics Data System (ADS)

The aim of this work is to describe the characteristics of internal waves in Todos Santos Bay, Baja California (Mexico) from direct measurements made during the international experiment Fluxes Linking the Offshore with the Onshore (FLOO), which was held in August 2007. Time series of temperature and currents were obtained in the study area, placing several submerged moorings with chains of HOBO-V2 sensors and an ADCP. In addition, transects were made using a towed undulating CTD system and chains of thermographs which had a response time of 5 s. These measurements allowed the construction of the scenarios of generation, propagation and disintegration of the internal tide in the Bay. The internal tidal waves are generated by the barotropic tide at the edge of the continental shelf north of the Bay, have wavelengths ~9 km, are distributed in the form of an arc, and travel to the southeast with phase velocity ~20 cm/s . In shallow waters near the coast, tidal waves are refracted and quickly divide into groups of short nonlinear internal waves with amplitudes 15-20 m, periods 5-20 minutes, and wavelengths 50-200 m. Nonlinear internal waves play an important role in the dynamics of Todos Santos Bay, especially in the coastal zone. During partial or total destruction, vertical mixing and sudden changes of stratification take place. This affects the formation of the vertical structure of water density and ocean biological productivity.

Filonov, A.; Lavin, M. F.; Ladah, L. B.; Tereshchenko, I.



Stress protein accumulation as an indicator of impact by the petroleum industry in Todos os Santos Bay, Brazil  

Microsoft Academic Search

The relative concentrations of stress proteins in juvenile mangrove oysters (Crassostrea rhizophorae), from six study sites in Todos os Santos Bay, Bahia, Brazil, were used to evaluate the possibility of chronic environmental impact induced by 40 years of exposure to the local petroleum industry. Replicated tissue samples from oysters at four sites associated with the extraction, transport and refinement of

I. A Nascimento; M. B. N Leite; G Sansone; S. A Pereira; D. H Smith



Assessment of oil refinery waste on Rhizophora mangle L. seedling growth in mangroves of Todos os Santos Bay, Bahia, Brazil  

Microsoft Academic Search

Seeds of Rhizophora mangle L. were planted and comparatively assessed in six mangroves of Todos os Santos Bay, Bahia, Brazil. Five of the mangroves were on the north of the bay, an area under the influence of oil activities: two areas around the Landulpho Alves Refinery in Mataripe, and in Madre de Deus Island, Pati Island, and Fontes Island. The

M. D. R Orge; I. J Porsché; M. C Costa; J. S Lima; S. E. D Soares; R Justino



Hepatozoon canis infecting dogs in the State of Espírito Santo, southeastern Brazil.  


From May 2007 to March 2008, blood samples were collected from 92 healthy dogs living in 21 households (17 farms in rural area, and 4 homes in urban area) in 6 counties of the State of Espírito Santo, southeastern Brazil. In addition, ticks were collected from these dogs. A mean of 4.4+/-3.0 dogs (range: 1-12) were sampled per household; 78 and 14 dogs were from rural and urban areas, respectively. Polymerase chain reaction (PCR) designed to amplify fragments of the 18S rDNA gene of Babesia spp or Hepatozoon spp revealed amplicons of the expected size in 20 (21.7%) dogs for Babesia, and 54 (58.7%) dogs for Hepatozoon. All Babesia-positive dogs were also Hepatozoon-positive. Among the 21 households, 15 (71.4%) from 3 counties had at least one PCR-positive dog, including 13 farms (rural area) and 2 homes (urban area). A total of 40 PCR products from the Hepatozoon-PCR, and 19 products from the Babesia-PCR were submitted to DNA sequencing. All generated sequences from Hepatozoon-PCR were identical to each other, and to corresponding 18S rDNA sequences of H. canis in GenBank. Surprisingly, all generated sequences from the Babesia PCR were also identical to corresponding 18S rDNA sequences of H. canis in GenBank. Dogs from 10 rural and 2 urban households were found infested by Rhipicephalus sanguineus ticks. Immature of Amblyomma cajennense ticks were found in dogs from only 4 rural households (also infested by R. sanguineus). All but one household with R. sanguineus-infested dogs had at least one Hepatozoon-infected dog. Statistical analysis showed that the presence of ticks (i.e. R. sanguineus) infesting dogs in the households was significantly (P<0.05) associated with at least one PCR-positive dog. There was no significant association (P>0.05) between PCR-positive dogs and urban or rural households. Canine hepatozoonosis caused by H. canis is a high frequent infection in Espírito Santo, Brazil, where it is possibly vectored by R. sanguineus. Since all infected dogs were found apparently healthy, the pathogenicity of H. canis for dogs in Espírito Santo is yet to be elucidated. PMID:19482427

Spolidorio, Mariana G; Labruna, Marcelo B; Zago, Augusto M; Donatele, Dirlei M; Caliari, Késia M; Yoshinari, Natalino H



Shellfish from Todos os Santos Bay, Bahia, Brazil: treat or threat?  


This study determined the concentrations of major and trace elements in shellfish (oysters, clams and mussels) and conducted an assessment of the health risks due to the consumption of contaminated seafood. Samples were collected at 34 sites along Todos os Santos Bay, Brazil. The elements were determined by ICP OES and Hg by Direct Mercury Analysis. Relatively high concentrations of trace elements (As, Zn, Se and Cu) were found in seafood tissues. Potential daily intake of As, Co, Se, Zn and Cu associated to shellfish consumption suggested relevant non-carcinogenic risk for all studied locations. Copper was the element that posed the greatest non-carcinogenic risk, while Pb posed the highest carcinogenic risk. Health risks for humans were greatest from the consumption of mussels. Contaminated shellfish offer the greatest risk for children, subsistence fishers and subsistence shellfish consumers. PMID:21803378

de Souza, Manuel M; Windmöller, Cláudia C; Hatje, Vanessa



Metal concentration in muscle of two species of flatfish from Santos Bay, Southeastern Brazilian coast  

NASA Astrophysics Data System (ADS)

The aim of this study was to investigate metals in muscles of two species of flatfish, using Particle Induced X-ray Emission (PIXE). Specimens were caught monthly throughout the year 2005. Sampling was done at six points in the Santos Bay under different anthropogenic influences. Analysis of 56 samples of muscle showed detectable amounts of Al, As, Pb, Cu, Cr, Fe, Sr, Mn, Hg, Ni, Se and Zn. Except Cu, there were no correlations with the levels of sediment contamination by metals and those found in muscles. Moreover, there were significant differences between juveniles and adults concerning the concentration of Al, Mn and As. According to Brazilian Health Surveillance Agency, some elements detected in the samples of muscle were above of permitted by law for consumption: Hg and Pb (in samples from the internal area), As (from the west side) and Se and Cr (from east side of the bay).

Rocha, M. L. F.; Dias, J. F.; Boufleur, L. A.; Santos, C. E. I.; Dias, J. F.



Ethical proceedings against dentists in Espírito Santo for infringements to the code of dental ethics.  


Just like any other profession, dentistry requires ethical and moral responsibilities that must be fulfilled, and the duty of every professional is to meet his obligations under the law. In light of the Código de Ética Odontológica (CEO - Code of Dental Ethics), this research aims to expound on the ethical violations committed by dentists in their practice, according to the ethical review process proposed by the Conselho Regional de Odontologia do Estado do Espírito Santo (CRO/ES - Regional Council of Dentistry of the State of Espírito Santo). The study is both retrospective and descriptive, using a quantitative approach. Data collection comprised all the ethical proceedings filed at the CRO/ES, between the years 2000 and 2011. Considering the 529 cases examined, the most frequent reason for infringement was illicit advertising (39.7%), followed by technical error (18.7%), irregular registration (16.8%), patient/professional relationship (11.3%) tax lien foreclosure (6.6%), professional/professional relationship (4.0%), irregular/illegal cover up practice (0.9%), consumer relations (0.8%), failure to provide care (0.6%), false certification (0.4%), and disrespect for authority and colleagues (0.2%). Most (59.2%) of the dental surgeons (DSs) involved were male, 35.5% were between 31 and 40 years of age, 85.8% had graduated over five (5) years prior, and 73.2% were general practitioners. Only 22 (4.2%) cases went to trial, resulting in 8 (36.4%) acquittals and 14 (63.6%) convictions. The most commonly applied penalties were private warning + pecuniary penalty (8 or 57.1%). It was concluded that the CEO must be followed with more discipline, and that professionals should seek information about their duties and obligations under the law, on behalf of patient welfare. PMID:24820427

Pacheco, Karina Tonini Dos Santos; Silva Junior, Manoelito Ferreira; Meireles, Naiara Ribeiro



Notes on African Haemaphysalis Ticks. X. H. (Kaiseriana) aciculifer Warburton and H. (K.) rugosa Santos Dias, the African Representatives of the Spinigera Subgroup (Ixodoidea: Ixodidae).  

National Technical Information Service (NTIS)

Haemaphysalis (Kaiseriana) aciculifer Warburton and H. (K.) rugosa Santos Dias have been confused in much literature on African ticks. The adult and immature stages of aciculifer are redescribed. The male of rugosa is described and the female redescribed;...

H. Hoogstraal K. M. El Kammah



Effects of dredging operations on sediment quality: contaminant mobilization in dredged sediments from the Port of Santos, SP, Brazil  

Microsoft Academic Search

Background, aim, and scope  Contaminated sediments are a worldwide problem, and mobilization of contaminants is one of the most critical issues in environmental\\u000a risk assessment insofar as dredging projects are concerned. The investigation of how toxic compounds are mobilized during\\u000a dredging operations in the channel of the Port of Santos, Brazil, was conducted in an attempt to assess changes in the

Ronaldo J. Torres; Denis M. S. Abessa; Fernando C. Santos; Luciane A. Maranho; Marcela B. Davanso; Marcos R. L. do Nascimento; Antonio A. Mozeto



Detecting Stress at the Whole-Ecosystem Level: The Case of a Mountain Lake (Lake Santo, Italy)  

Microsoft Academic Search

Detecting the early signs of stress is imperative for the conservation of natural ecosystems. They may, however, go unrecognized\\u000a because ecosystems, when disturbed, may act as sinks that absorb the external impact without showing any evident changes.\\u000a This seems to be the case for Lake Santo, a small water body located in a mountainous area of northern Italy. Tourism activity

Cristina Bondavalli; Antonio Bodini; Giampaolo Rossetti; Stefano Allesina



Sedimentologic and geomorphic evidence for seesaw subsidence of the Santo Domingo accommodation-zone basin, Rio Grande rift, New Mexico  

Microsoft Academic Search

Basin analysis of facies patterns and ba- sin geomorphology reveals a complex sub- sidence pattern for part of the Rio Grande rift in north-central New Mexico, United States. The Santo Domingo basin study site is located in the accommodation zone be- tween the east-tilted northern Albuquerque basin and the west-tilted Espanola basin half-graben. Upper Miocene through lower Pleistocene basin-fill sediment

Gary A. Smith; William McIntosh; Andrika J. Kuhle



Survey for Tick-Borne Zoonoses in the State of Espirito Santo, Southeastern Brazil  

PubMed Central

Blood samples collected from 201 humans, 92 dogs, and 27 horses in the state of Espirito Santo, Brazil, were tested by polymerase chain reaction, indirect immunofluorescence assays, and indirect enzyme-linked immunosorbent assay for tick-borne diseases (rickettsiosis, ehrlichiosis, anaplasmosis, borreliosis, babesiosis). Our results indicated that the surveyed counties are endemic for spotted fever group rickettsiosis because sera from 70 (34.8%) humans, 7 (7.6%) dogs, and 7 (25.9%) horses were reactive to at least one of the six Rickettsia species tested. Although there was evidence of ehrlichiosis (Ehrlichia canis) and babesiosis (Babesia canis vogeli, Theileria equi) in domestic animals, no human was positive for babesiosis and only four individuals were serologically positive for E. canis. Borrelia burgdorferi-serologic reactive sera were rare among humans and horses, but encompassed 51% of the canine samples, suggesting that dogs and their ticks can be part of the epidemiological cycle of the causative agent of the Brazilian zoonosis, named Baggio-Yoshinari Syndrome.

Spolidorio, Mariana G.; Labruna, Marcelo B.; Machado, Rosangela Z.; Moraes-Filho, Jonas; Zago, Augusto M.; Donatele, Dirlei M.; Pinheiro, Sonia R.; Silveira, Iara; Caliari, Kesia M.; Yoshinari, Natalino H.



Determinants of health in seasonal migrants: coffee harvesters in Los Santos, Costa Rica.  


In the agroexport zone of Los Santos Zone in Costa Rica, coffee is harvested by migrant labor. Most migrants are from Panama and Nicaragua. We describe migrants' housing- and service-related health determinants, with analyses of ethnicity, nationality and geography. We used interviews, observation-based assessments, and the Geographic Information System to assess a population of 8,783 seasonal migrants and 1,099 temporary dwellings at a total of 520 farms during 2004-2005. We identified determinants of poor health including widespread deficiencies in the quality of grower-provided dwellings, geographical isolation, crowding, lack of radio and television, and deficient toilets and cooking facilities. The indigenous and non-Costa Ricans shared the poorest conditions. Reluctance to use mainstream public health services was widespread, especially among foreign and indigenous migrants and the geographically isolated. Post-study, researchers organized workshops for audiences including workers, coffee producers, public officials and service providers. Topics have included migration, preventive health and hygiene, and child labor. This work was successful in convincing Costa Rican social security authorities to implement reforms that improve access to and quality of health care for the migrants. Special projects on ergonomics, psychosocial health hazards, and water quality, as well as a literacy program, are ongoing. PMID:18507290

Loría Bolaños, Rocío; Partanen, Timo; Berrocal, Milena; Alvárez, Benjamín; Córdoba, Leonel



Seasonal variation of the upwelling trap in the Todos Santos Bay, Baja California  

NASA Astrophysics Data System (ADS)

Todos Santos Bay is located on the Pacific coast of Baja California, a region characterized by coastal upwelling throughout the year, being more intense in spring and summer. Aquaculture activities are expanding in the bay, so it is important to understand the processes that modulate the water temperature and stratification, given that these in turn affect biological productivity. This work presents an analysis of the seasonal variation of the temperature of the bay and adjacent regions, with satellite data and CTD casts made in quarterly cruises between 2008-2012. It has been observed that, on average, the surface temperature in the bay is warmer than outside, suggesting that upwelled waters that enter the bay are trapped a sufficiently long time to be heated by solar radiation, hence the name upwelling trap. During upwelling events that drive cold waters to the surface in the regions adjacent to the bay, satellite images show that the surface temperature inside the bay can be 4-5 °C higher than the surrounding area. This thermal front is strongest in summer when solar radiation is greater. We have studied the vertical structure of the lens of warm water with CTD data, which shows that its depth does not exceed 10 m and the temperature is 3 to 4 °C warmer than the water below. Preliminary results on the processes involved in the formation and evolution of the upwelling trap at synoptic to monthly scales will be presented, based on the analysis of sea surface temperature images, winds, and currents in the northwestern entrance of the bay.

Calva, M. A.; Perez-Brunius, P.; Lopez, M.; Candela, J.; García, E.



Mountain-Block Recharge in the Santo Tomas Valley, Baja California, Mexico  

NASA Astrophysics Data System (ADS)

Mountain block recharge (MBR) to adjacent basin aquifers can be a significant source of groundwater in arid and semi-arid regions. Unfortunately, geologic complexities within the mountain block often limit our understanding of this indirect form of recharge. Secondary permeability, resulting from faults and fractures, allows rainwater to infiltrate crystalline mountain rock, ultimately recharging the basin aquifer. Therefore, it is essential to consider mountain block geological features, especially faults, in recharge studies. We attempt to better understand MBR by creating a detailed fracture-trace map and by sampling springs and groundwater throughout the Santo Tomas valley, located in Northern Baja California, Mexico. Bounded by active faults, the valley is heavily fractured. These fractures enable fluid flow within the mountain block. Stable isotope (?18O and ?2H) data show two distinct types of spring water within the watershed, possibly representing local and regional flow paths. Thermal springs, believed to be regional flow, display a -1.9‰ ?18O depletion when compared to all other spring water, indicative of recharge from higher elevations or older waters; both of the latter would be less affected under local drought conditions. This distinct isotopic signal was found 15 km downstream in the alluvial aquifer, indicating a significant amount of water is recharging the basin aquifer via the mountain block along this flow regime. A quantitative permeability value for the faults and fracture zones is difficult to attain due to their heterogeneous nature. However, the thermal system and most cold-water springs surface along active faults, which appear to transmit more water than undifferentiated fractures. Stable isotope (O and H) data. An isotopic distinction can be seen between the hot and cold springs within the watershed. Note that all the spring samples are taken between 400 - 550 m elevation, which includes the hot springs.

Thomas, W. M.; Kretzschmar, T.



Feasibility Study of Economics and Performance of Solar Photovoltaics at the Santo Domingo Pueblo in Sandoval County, New Mexico  

SciTech Connect

The U.S. Environmental Protection Agency (EPA), in accordance with the RE-Powering America's Land initiative, selected the Pueblo of Santo Domingo in Sandoval County, New Mexico, for a renewable energy production feasibility study. The National Renewable Energy Laboratory (NREL) provided technical assistance for this project. The purpose of this report is to assess specific areas on the Pueblo for potential installation of photovoltaic (PV) systems and to estimate the cost, performance, and site impacts of different PV options. The report also recommends financing options that could assist in the implementation of these PV systems.

Geiger, J.; Lisell, L.; Mosey, G.



Effects of ayahuasca on psychometric measures of anxiety, panic-like and hopelessness in Santo Daime members.  


The use of the hallucinogenic brew ayahuasca, obtained from infusing the shredded stalk of the malpighiaceous plant Banisteriopsis caapi with the leaves of other plants such as Psychotria viridis, is growing in urban centers of Europe, South and North America in the last several decades. Despite this diffusion, little is known about its effects on emotional states. The present study investigated the effects of ayahuasca on psychometric measures of anxiety, panic-like and hopelessness in members of the Santo Daime, an ayahuasca-using religion. Standard questionnaires were used to evaluate state-anxiety (STAI-state), trait-anxiety (STAI-trait), panic-like (ASI-R) and hopelessness (BHS) in participants that ingested ayahuasca for at least 10 consecutive years. The study was done in the Santo Daime church, where the questionnaires were administered 1h after the ingestion of the brew, in a double-blind, placebo-controlled procedure. While under the acute effects of ayahuasca, participants scored lower on the scales for panic and hopelessness related states. Ayahuasca ingestion did not modify state- or trait-anxiety. The results are discussed in terms of the possible use of ayahuasca in alleviating signs of hopelessness and panic-like related symptoms. PMID:17532158

Santos, R G; Landeira-Fernandez, J; Strassman, R J; Motta, V; Cruz, A P M



A study of multidrug-resistant tuberculosis in risk groups in the city of Santos, S?o Paulo, Brazil  

PubMed Central

Monitoring the extent of and trends in multidrug-resistant tuberculosis (MDR-TB) is a priority of the Brazilian National Tuberculosis Control Programme. The current study aimed to estimate the incidence of MDR-TB, describe the profile of TB drug resistance in risk groups and examine whether screening for MDR-TB adhered to the recommended guidelines. A descriptive study that examined diagnosed cases of pulmonary TB was conducted in the city of Santos, Brazil, between 2000–2004. Of the 2,176 pulmonary TB cases studied, 671 (30.8%) met the criteria for drug sensitivity testing and, of these cases, 31.7% (213/671) were tested. Among the tested cases, 9.4% were resistant to one anti-TB drug and 15% were MDR. MDR was observed in 11.6% of 86 new TB cases and 17.3% of 127 previously treated cases. The average annual incidence of MDR-TB was 1.9 per 100,000 inhabitants-years. The extent of known MDR-TB in the city of Santos is high, though likely to be underestimated. Our study therefore indicates an inadequate adherence to the guidelines for MDR-TB screening and suggests the necessity of alternative strategies of MDR-TB surveillance.

Coelho, Andrea Gobetti Vieira; Zamarioli, Liliana Aparecida; Telles, Maria Alice; Ferrazoli, Lucilaine; Waldman, Eliseu Alves



Sand fly vectors (Diptera, Psychodidae) of American visceral leishmaniasis areas in the Atlantic Forest, State of Espírito Santo, southeastern Brazil.  


The aim of this study was to evaluate the sand fly fauna of American visceral leishmaniasis (AVL) endemic areas within the Central Atlantic Forest Biodiversity Corridor, State of Espírito Santo, southeastern Brazil. The sand fly captures were performed between January, 1989 and December, 2003 in localities where autochthonous cases of AVL were recorded, as well as in their boundary areas. Sand flies were collected from surrounding houses and domestic animal shelters using two to five CDC automatic light traps, and manual captures were also performed using mouth aspirators in one illuminated Shannon trap during the first four hours of the night. We used cladistic analysis to determine the geographic relationships among the collected sand fly species as well as the index species for the occurrence of other sand flies. A total of 62,469 sand flies belonging to 17 species and eight genera was collected in 164 localities from nine municipalities with AVL records. The richness (S=17) and diversity (H=0.971) of sand flies were lower than in conservation areas and similar to modified environments in the Atlantic Forest of Espírito Santo. Lutzomyia longipalpis was identified in 79 localities. The cladistic analysis identified Evandromyia lenti as the index species for Lutzomyia longipalpis. The latter seems to be the main vector of AVL in the Central Atlantic Forest Biodiversity Corridor due to its high abundance and distribution matching the disease occurrence. Therefore, Evandromyia lenti may be used as an index species for the occurrence of Lutzomyia longipalpis. PMID:22548541

Pinto, Israel de Souza; Ferreira, Adelson Luiz; Valim, Valéria; Carvalho, Felipe dos Santos; da Silva, Giovana Marques; Falcão, Alda Lima; Dietze, Reynaldo; Falqueto, Aloísio



Molecular characterization of noroviruses and HBGA from infected Quilombola children in Espirito Santo State, Brazil.  


Noroviruses (NoV) are the main etiological agents of gastroenteritis outbreaks worldwide and susceptibility to NoV infection has been related to the histo-blood group antigen (HBGA). This study aimed to determine the prevalence of NoV strains and to evaluate the HBGA phenotype and genotype of children from semi-isolated Quilombola communities, descendents of black slaves in Brazil. A total of 397 children up to eleven years old, with and without diarrhea, from Quilombola Communities in the Espirito Santo State, Brazil, were investigated for the presence of NoV from August 2007 to September 2009. Feces were collected from all the children, and blood from the NoV positive children. NoV was screened by reverse transcription-PCR with primers for the RNA-dependent RNA polymerase region; genogroup was determined by PCR with primers for the C and D regions and genotyped by sequencing. HBGA phenotype was performed by gel-spinning and FUT2 and FUT3 were analyzed by PCR or sequencing analysis. NoV were detected in 9.2% (12/131) of diarrheic and 1.5% (4/266) of non-diarrheic children (p<0.05, Fisher's exact test). GI and GII genogroups were present in 12.5% and 87.5% of the samples, respectively. The following genotypes were characterized: GII.4 (25%), GII.12 (25%), GII.6 (12.5%) and GI.1 (6.3%), GI.3 (12.5%) and GI.4 (6.3%). Children infected with NoV showed the A (n?=?6), O (n?=?6), and B (n?=?2) HBGA phenotypes, and 13 of them were classified as secretors (Se) and one as a non secretor (se). Mutations of Se (40), (171,216,357,428,739,960) were found for the FUT2 gene and mutations of Le (59, 202, 314) for the FUT3 gene. The only se child was infected by NoV GI, whereas the Se children were indiscriminately infected by GI or GII. This study showed rates of NoV infection in symptomatic and asymptomatic Quilombola children consistent with other studies. However, children under 12 months were seven times more affected than those between 1 and 5 years old. GII.12 was as frequent as GII.4 and GI.1 and GI.4 were described for the first time in Brazil. Owing to the small number of cases studied, no clear pattern of susceptibility and/or HBGA resistance could be inferred. PMID:23894453

Vicentini, Fernando; Denadai, Wilson; Gomes, Yohanna Mayelle; Rose, Tatiana L; Ferreira, Mônica S R; Le Moullac-Vaidye, Beatrice; Le Pendu, Jacques; Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira; Spano, Liliana Cruz



Apatite Fission-Track Analysis of the Middle Jurassic Todos Santos Formation from Chiapas, Mexico.  

NASA Astrophysics Data System (ADS)

The Sierra de Chiapas (SCH), located in the south of Mexico, is a complex geological province that can be divided on four different lithological or tectonic areas: (1) the Chiapas Massif Complex (CMC); (2) the Central Depression; (3) the Strike-slip Fault Province, and (4) the Chiapas Fold-and-thrust Belt. The CMC mostly consists of Permian granitoids and meta-granitoids, and represents the basement of the SCH. During the Jurassic period red beds and salt were deposited on this territory, related to the main pulse of rifting and opening of the Gulf of Mexico. Most of the Cretaceous stratigraphy contains limestones and dolomites deposited on a marine platform setting during the postrift stage of the Gulf of Mexico rift. During the Cenozoic Era took place the major clastic sedimentation along the SCH. According the published low-temperature geochronology data (Witt et al., 2012), SCH has three main phases of thermo-tectonic history: (1) slow exhumation between 35 and 25 Ma, that affected mainly the basement (CMC) and is probably related to the migration of the Chortís block; (2) fast exhumation during the Middle-Late Miocene caused by strike-slip deformation that affects almost all Chiapas territory; (3) period of rapid cooling from 6 to 5 Ma, that affects the Chiapas Fold-and-thrust Belt, coincident with the landward migration of the Caribbean-North America plate boundaries. The two last events were the most significant on the formation of the present-day topography of the SCH. However, the stratigraphy of the SCH shows traces of the existence of earlier tectonic events. This study presents preliminary results of apatite fission-track (AFT) dating of sandstones from the Todos Santos Formation (Middle Jurassic). The analyses are performed with in situ uranium determination using LA-ICP-MS (e.g., Hasebe et al., 2004). The AFT data indicate that this Formation has suffered high-grade diagenesis (probably over 150 ºC) and the obtained cooling ages, about 70-60 Ma, correspond to a Late Cretaceous event. This tectonic event is contemporaneous with a startup of the Laramide Orogeny occurred in North America. The constructed time-temperature paths show the rapid cooling during the Middle-Late Miocene (15-10 Ma), like other published data. References: Hasebe et al. (2004) Chemical Geology, 207, 135-145 Witt et al. (2012) Tectonics, 31, TC6001, doi:10.1029/2012TC003141

Abdullin, Fanis; Solé, Jesús; Shchepetilnikova, Valentina; Solari, Luigi; Ortega-Obregón, Carlos



A Future for the Excluded. Job Creation and Income Generation by the Poor: Clodomir Santos de Morais and the Organization Workshop.  

ERIC Educational Resources Information Center

This book, translated from Spanish, contains 20 chapters by various authors examining and expanding on the work of Clodomir Santos de Morais in educating and empowering the poor, mostly in Latin America, for entrepreneurship. One of the chapters is by de Morais. The following are included, organized in four sections: "Those Who Don't Eat and Those…

Carmen, Raff, Ed.; Sobrado, Miguel, Ed.


Diel movement patterns of the scalloped hammerhead shark ( Sphyrna lewini ) in relation to El Bajo Espiritu Santo: a refuging central-position social system  

Microsoft Academic Search

Movement patterns of scalloped hammerhead sharks in the vicinity of El Bajo Espiritu Santo, a seamount in the Gulf of California, were determined by tracking by ultrasonic telemetry 13 sharks and marking 100 sharks. The 13 tracked sharks swam back and forth along the seamount ridge throughout the day. They did not swim in different directions to reduce swimming effort

A. Peter Klimley; Donald R. Nelson



Integration of the PSI technique and conventional ground-based methods for landslide characterization and monitoring: the case study of Santo Stefano d'Aveto; Northern Apennines, Italy  

Microsoft Academic Search

Santo Stefano village is located in the Northern Apennines (Italy) and is built up on an ancient landslide, defined as a complex phenomenon that is an earth rotational slide evolving into a flow. The landslide has an extension of 1,3 km2 and a volume of about 10 million of m3. The landslide can be defined as active and according to

Veronica Tofani; Valeria Pancioli; Filippo Catani; Nicola Casagli



Characterization of the benthic environment of a coastal area adjacent to an oil refinery, Todos os Santos Bay (NE-Brazil)  

Microsoft Academic Search

A B S T R A C T In order to determine the sedimentological and physico-chemical characteristics of the benthic environment and also the level, distribution and probable sources of aliphatic and polycyclic aromatic hydrocarbons, sediment samples were collected at 28 stations in the northeast portion of Todos os Santos Bay. The centre and the east region of the study

Natalia Venturini; Luiz Roberto Tommasi; Márcia C. Bícego; César C. Martins



Perspectivas no tratamento medicamentoso da obesidade Perspectives of drug treatment of obesity  

Microsoft Academic Search

The perspectives in the pharmacological treatment of obesity can be classified in two classes: drugs already in the market, in advanced clinical trial or in final approval, or drugs in early development. Among the first class are antiepileptic drugs like topiramate (although it was studied for obesity treatment it was descontinued for this indication because of the high drop-out rate

Alfredo Halpern; Marcio C. Mancini


FPGA implementation of Santos-Victor optical flow algorithm for real-time image processing: an useful attempt  

NASA Astrophysics Data System (ADS)

A FPGA based hardware implementation of the Santos-Victor optical flow algorithm, useful in robot guidance applications, is described in this paper. The system used to do contains an ALTERA FPGA (20K100), an interface with a digital camera, three VRAM memories to contain the data input and some output memories (a VRAM and a EDO) to contain the results. The system have been used previously to develop and test other vision algorithms, such as image compression, optical flow calculation with differential and correlation methods. The designed system let connect the digital camera, or the FPGA output (results of algorithms) to a PC, throw its Firewire or USB port. The problems take place in this occasion have motivated to adopt another hardware structure for certain vision algorithms with special requirements, that need a very hard code intensive processing.

Cobos Arribas, Pedro; Monasterio Huelin Maciá, Félix



The effect of iron-ore particles on the metal content of the brown alga Padina gymnospora (Espírito Santo Bay, Brazil).  


The iron-ore particles discharged by a pellet processing plant (Espírito Santo Bay, Brazil) cover the seabed of Camburi Beach and consequently, the epibenthic community. In order to determine the importance of the contribution of the iron-ore deposits to the metal concentration in macroalgae of Espirito Santo Bay, four methods of cleaning particulate material adhered to the surface of thalli were tested prior to metal tissue analysis (Al, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) of Padina gymnospora. In addition, heavy metal concentrations were determined in individuals of P. gymnospora from a site (Frade Island) not affected by the iron-ore particles. The most efficient cleaning treatment, a combination of scraping and washing with an ethanol-seawater solution (NA+SC+ET) removed a number of particles on the surface of thalli 10 times higher than that observed in the control (C). Using this treatment, the total-metal concentrations were reduced by 78% for Fe and 50% for Al respect to the control. However, Fe, Al and Cu concentrations after treatment NA+SC+ET were significantly higher than those found at Frade Island. It is suggested that the iron-ore deposit might be a source for metal availability to macroalgae exposed to the dumped material at Espirito Santo Bay. PMID:12628209

Nassar, Cristina A G; Salgado, Leonardo T; Yoneshigue-Valentin, Yocie; Amado Filho, Gilberto M



Ar/Ar Dates From Tarrafal, Santo Antao, Cape Verde: Re-evaluation of two Brunhes age Excursions  

NASA Astrophysics Data System (ADS)

We present new 40Ar/39Ar ages from a 500 m thick sequence of basaltic lava flows on the island of Santo Antão, Cape Verde, and assess their implications for two previously reported, fully reversed excursions in the Brunhes chron (Knudsen et al., Earth Planet. Sci. Lett., 206, 199-214, 2003). Paleodirectional experiments on a sequence of 52 lava flows at Tarrafal, Santo Antão, coupled with two argon dates, one from the bottom and one from near the top of the sequence, suggested that the majority of the volcanic stratigraphy was emplaced between 0.54 ± 0.09 Ma and 0.38 ± 0.05 Ma and that the two excursions could be related to geomagnetic instabilities within the Brunhes Chron that had not previously been observed in terrestrial volcanic records. New 40Ar/39Ar isochron ages, determined using the incremental heating method at the University of Wisconsin-Madison for nine groundmass separates from hand samples collected by Knudsen et al. (2003), range from 5.04 ± 0.10 Ma and 2.57 ± 0.02 Ma and indicate that that the stratigraphy of the Tarrafal section is more complex than originally thought. This more complicated stratigraphy can be explained by infilling of a flank collapse structure with younger Brunhes age lava flows and subsequent erosion of the sequence. The lower "excursion" reflects sampling across two normally magnetized Brunhes age flows at the base of the section and an older sequence of flows encompassing both reversed and normal polarity. The older reversed flows correspond to part of the Gilbert chron between 5.04 ± 0.10 Ma and 4.53 ± 0.09 Ma; the normal flows could relate to a normal polarity chron or subchron between 4.53 ± 0.09 Ma and 2.8 ± 0.02 Ma. The upper "excursion" marks the change from a volcanic event of reversed polarity (3.56 ± 0.29 Ma) to the normal polarity flows of the Brunhes chron (0.41 ± 0.02 Ma). Our findings further highlight the need for precise age determinations and a high sampling density of dated flows when using long lava sequences to reconstruct paleofield behaviour.

Knudsen, M. F.; Brown, M. C.; Singer, B. S.; Feinberg, J. M.



The long-living Cao Grande magmatic system on Santo Antão, Cape Verde Islands - A revised model  

NASA Astrophysics Data System (ADS)

Santo Antão represents an ocean island shield volcano in its early post-erosional stage. At this stage, mafic Strombolian volcanic activity had been replaced by Pleistocene highly explosive phonolitic eruptions. Two phonolitic tephra successions, Cão Grande Pumice units I and II (CG I and CG II), were previously identified and described (Holm et al. 2006; Mortensen et al. 2009). Our tephrostratigraphic fieldwork on Santo Antão identified a third older explosive phonolitic phase (CG 0) and two phonolitic domes. While CG 0 and CG I are separated by an erosional unconformity, CG II succeeds directly on CG I. This is also assisted by our preliminary 40Ar/39Ar ages that overlap for CG I and CG II and indicate a break in eruptive history of c. 40 ka between CG 0 (206 × 8 ka) and CG I (163 × 15 ka). Based on XRF bulk rock analyses Holm et al. (2006) assigned CG I and CG II to two discrete magmatic series, a nephelinite - phonolite trend and a basanite - phonolite trend respectively. According to this, our data shows that the older CG 0 is also related to the basanite - phonolite series. Although field observations and geochronology indicate that CG I and CG II erupted in rapid succession from the same area around the Monte Figueira dome, there is no evidence for direct interaction of both magmatic systems. Using major and trace element glass compositions we can show that all three units have distinct geochemical compositions but the initial stage of CG II can be related compositionally to CG 0. This suggests that both units derived from the same magmatic system, which is also supported by thermobarometry data displaying similar temperatures and pressures around 970 °C and 900 MPa for CG 0 and CG II and significant lower values (900 °C; 500 MPa) for CG I. Detailed stratigraphic linked glass analyses, representing the latest stage of magma evolution, show uniform compositions for CG I and therefore question the established magma chamber model of CG I from Mortensen et al. (2009) that is only based on XRF bulk rock analyses. Furthermore it indicates multiple magma chamber replenishments within the CG II unit. We will present new insights into the magma chamber conditions of both magmatic series of the Cão Grand Complex including a magma chamber model for all three units, based on major and trace element bulk rock composition using XRF and ICP-MS methods as well as major and trace element compositions of glass and mineral analyses by EMP.

Eisele, S.; Freundt, A.; Kutterolf, S.; Ramalho, R. S.



Environmental analyses of the parasitic profile found in the sandy soil from the Santos municipality beaches, SP, Brazil.  


The environmental contamination by geohelminths represents a world public health problem and has been well documented by several authors. However, few papers describe the presence of such contamination in saline soils of coastal beaches. A study was performed on the beaches of the municipality of Santos in the period between May 2004 to April 2005 with the aim of determining the degree of contamination, and the correlation between contamination level and seasonal conditions and characteristics of the environment. Of the 2,520 samples analyzed, 18.2% (458) were contaminated, 32.3% (148) of which were localized in children's recreational areas (playgrounds). The parasite profile found in the analyzed samples indicated the presence of several zoonotic parasites: Ancylostoma larvae (82.5%), Toxocara sp. eggs (59.4%), Ancylostomidae-like eggs (37.1%), coccid oocysts (13.5%), Trichostrongylus sp. eggs and larvae, Ascaris lumbricoides eggs, (11.6%), Entamoeba sp. cysts (10.0%), Strongyloides sp. (4.8%), several free nematoids and some non-identified parasitic structures (3.3%). It was established that the highest frequency of parasitic structures occurred in the months between May and October 2004, and from February to March 2005. An increase in the diversity of parasitic forms was documented in the months between February to December 2004 and from January to April 2005, these periods having the highest rainfall. PMID:22012454

Rocha, Silvana; Pinto, Rosa Maria Ferreiro; Floriano, Aline Petrollini; Teixeira, Lais Helena; Bassili, Bianca; Martinez, Araceles; Costa, Sergio Olavo Pinto da; Caseiro, Marcos Montani



[Access to the diagnosis of tuberculosis in health services in the municipality of Vitoria, state of Espírito Santo, Brazil].  


This study sought to assess the accessibility to the diagnosis of tuberculosis in health services in Victoria, state of Espírito Santo. It featured a cross-sectional study conducted in 2009 of patients with tuberculosis using the Primary Care Assessment Tool and statistical analysis with the Chi-square test (p <0,05). In relation to initial access to care, it was seen that the health service of first access most sought was Primary Care (37.6%), with most diagnoses occurring in the Tuberculosis Control Program Reference Units (61.3%). There was evidence of association between first health service accessed and the factors of time delay in: obtaining consultation at the first health service sought (p = 0.0182); diagnosis made by the first health service sought (p = 0.0001); request for sputum exam (p = 0,0003); request for X-ray exams (p = 0.0159); referral for X-rays at another institution (p = 0.0001); diagnosis by the same health service (p = 0.0001); exams conducted by the same health service that initially diagnosed tuberculosis (p = 0.0018); and proximity to the home (p = 0.0001). Therefore, the identification of important gaps in accessibility to diagnosis of tuberculosis seems to be related to the operational difficulties of organization of health care. PMID:24820606

Loureiro, Rafaela Borges; Villa, Tereza Cristina Scatena; Ruffino-Netto, Antônio; Peres, Renata Lyrio; Braga, Jose Ueleres; Zandonade, Eliana; Maciel, Ethel Leonor Noia



Lysosomal responses as a diagnostic tool for the detection of chronic petroleum pollution at Todos os Santos Bay, Brazil.  


Coastal marine environments, especially semienclosed systems such as bays, are under unrelenting stress caused by urban and industrial development. Biomonitoring plays a vital role in strategies to identify, assess, and control stressors. However, due to the magnitude of the challenge there is a demand for new and innovative approaches to provide timely and accessible information to environmental managers and policy makers. The present work aimed to assess hydrocarbon levels in sediments from petroleum-related industrial areas at Todos os Santos Bay (Brazil) and associate them to toxicity-induced responses (neutral red retention (NRR) assay) by the burrowing clam Anomalocardia brasiliana. Surface sediments collected during the dry and rainy seasons were analyzed for aliphatic and aromatic hydrocarbons. At the control site, hydrocarbon levels were low and mainly biogenic. The aliphatic hydrocarbon ("total unresolved complex mixture," alkanes, and isoprenoids) concentrations indicated a chronic situation with very little "fresh" oil contamination at the oil-related sites. The polycyclic aromatic hydrocarbons indicated sites moderately contaminated by chronic oil and some pyrolytic input. The effects of those contaminants were assessed by the lysosomal NRR assay applied to A. brasiliana hemocytes. Sediment toxicity at the oil-related sites was evidenced by the lowered capacity of the lysosomes to retain the neutral red dye compared to results from the control site. This research indicates that the NRR assay is a useful and efficient screening technique able to discriminate polluted from clean sites. PMID:16307981

Martins, L K P; Nascimento, I A; Fillmann, G; King, R; Evangelista, A J A; Readman, J W; Depledge, M H



Scale-relationships and geometry of normal faults reactivated during gravitational gliding of Albian rafts (Espírito Santo Basin, SE Brazil)  

NASA Astrophysics Data System (ADS)

Three-dimensional (3D) seismic-reflection data from SE Brazil are used to investigate the geometry and significance of normal faults reactivated during gravitational gliding of Albian rafts. Late Cretaceous gravitational instability in the study area led to the fragmentation of Albian strata in individual rafts separated by listric (roller) faults and associated sub-basins (Stage 1). Renewed gravitational gliding caused Albian rafts to be translated downslope until salt welds were formed and Albian strata became grounded over pre-salt sequences (Stage 2). Diachronous grounding promoted the reactivation of faults and folds, which at places developed into pop-up structures and broad anticlines sub-parallel to the strike of individual rafts (Stage 3). In this paper we present a new methodology to identify and date reactivated (i.e. shortened) fault families. Statistical analyses of six main fault families confirm that fault reactivation was diachronous in the study area, and its cessation depended on: i) the complete grounding of Albian rafts or, ii) gravitational stabilisation of the rafts imposed by sediment loading and cessation of tectonic tilting of the slope. The results of this study show that tectonically generated pulses of compression and uplift, commonly related to the Andean Orogeny, are not the only mechanisms deforming post-salt overburden units in proximal extensional-dominated regions of the southeast Brazilian margin. This is a crucial piece of information for geodynamic reconstructions and hydrocarbon exploration, as the close control of Albian rafts on fault reactivation induced a continuum of overburden deformation in the Espírito Santo Basin, rather than discrete episodes of inversion.

Alves, Tiago M.



Further Evidence for a new Brunhes Chron Excursion Recorded in Lava Flows at Agua Nova, Santo Antao, Cape Verde  

NASA Astrophysics Data System (ADS)

New paleodirectional and initial paleointensity results from a re-sampled Brunhes age sequence of 34 basaltic lava flows at Agua Nova, Santo Antão, Cape Verde are linked with precise ages determined using the 40Ar/39Ar incremental heating method on groundmass separated from oriented hand samples originally studied by Knudsen et al. (Earth Planet. Sci. Lett., 206, 199-214, 2003). Alternating field demagnetization experiments on one sample per flow by Knudsen et al. (2003) suggested that two directional excursions may have been recorded. Five new 40Ar/39Ar isochron ages, which bracket and include the two excursional flows, are indistinguishable from one another and imply that a single complex period of excursional behavior took place at about 480 ± 13 ka. This excursion is the first to be revealed in a terrestrial volcanic sequence of this age and could correspond to cryptochron C1n-1 (504 to 493 ka) in the marine magnetic anomaly record (Cande and Kent, J. Geophys. Res., 100, 6093-6095, 1995). It is too young to be associated with the widely recorded Big Lost excursion, which is 40Ar/39Ar dated at 579 ± 6 ka, or any of the five West Eifel excursions dated between 722 and 528 ka (Singer et al., Phys. Earth Planet. Inter., in press, 2008). Neither is it clearly correlative with significant directional or paleointensity features observed in several high sedimentation rate ODP cores. Accurate paleodirections and paleointenities will enable us to define the full vector behavior of the geomagnetic field during this brief period of geomagnetic instability and will help us to better understand the processes within the Earth's core that give rise to such events.

Brown, M. C.; Singer, B. S.; Knudsen, M. F.; Feinberg, J. M.



Salt roller growth as a controlling factors of the Albian rafts on Espírito Santo Basin, SE Brazil.  

NASA Astrophysics Data System (ADS)

The SE Brazil continental margin is dominated by continental-slope embankment, incision of submarine channel system and significant mass-gravitational processes, with salt tectonics playing a significant role in upper-crust deformation. It is known to comprise a margin rich in oil and gas, in which raft tectonics plays an important role in their migration and accumulation. A combination of 3D pre-stack and post-stack time-migrated (PSTM) data was used to interpret the evolution of the Albian rafts in Espírito Santo Basin, SE Brazil was used in this work to: a) investigate the impact of continuing halokinesis in raft movement and, b) critically assess the parameters considered to control raft tectonics. In the study area Albian rafts, which are laterally constrained by salt rollers that were reactivated during the Late Cretaceous-Cenozoic, are seen to be ramped over triangular salt structures, and rotated by growing salt pillows. As a result, we observe complex compartmentalization styles in the rafts, with three main types of faults being recognised in four distinct rafts: a) rollover faults formed axially to large N-S rafts, b) radial faults to segments of rafts that record torsion and enhanced downslope movement, c) reactivated crestal and keystone faults formed on the flanks of growing salt diapirs onto which rafts are ramped upon. The space between the rafts was also analysed and quantified and show to be influenced by the weight of the sediments overburden. This work shows that slope gradient and overburden thickness had little control on raft segmentation. We interpret these data as a proof that underlying salt was a primary control on raft tectonics in the study area, overriding the effect of overburden thickness and variable slope gradients on the rafts' internal compartmentalisation. This study will show different styles of compartmentalization observed in Albian rafts from SE Brazil.

Piedade, Aldina; Alves, Tiago



Climate Patterns Governing the Presence and Permanence of Salmonellae in Coastal Areas of Bahia de Todos Santos, Mexico?  

PubMed Central

Despite the importance of salmonellae as one of the major causes of food-borne infections worldwide, data regarding the presence of these organisms in the environment are limited. We investigated the presence of Salmonella spp. in Bahia de Todos Santos (Baja California, Mexico) and evaluated the environmental factors that affect the occurrence of Salmonella spp. in this arid region. A total of 1,331 samples collected from 21 sites along the coast during a period of 3 years were analyzed for Salmonella spp. Geographical and seasonal distribution of Salmonella spp. was evaluated in association with environmental parameters and with human infections in the area. The incidence of Salmonella bacteria throughout the study was 4.8%, with the highest incidence detected in wastewater (16.2%), followed by stream water (10.6%), mollusks (7.4%), and seawater (2.3%). Twenty different serotypes were identified among the 64 Salmonella isolates. The dominant serotype was Typhimurium (23.4%), followed by Vejle (6.2%). The presence of Salmonella spp. in coastal areas was mostly confined to rainy periods and areas of stream discharges, and runoff was identified as the predominant factor influencing the transport of Salmonella bacteria from source points to the sea via streams. Isolation of Salmonella spp. was negatively and significantly associated with temperature, probably because of the effect of solar radiation in the decline of permanence of Salmonella bacteria. Conversely, human infections prevailed during the warmest months and were negatively correlated with the presence of Salmonella spp. in the marine environment.

Simental, Lourdes; Martinez-Urtaza, Jaime



Geological setting and paleomagnetism of the Eocene red beds of Laguna Brava Formation (Quebrada Santo Domingo, northwestern Argentina)  

NASA Astrophysics Data System (ADS)

The red bed succession cropping out in the Quebrada Santo Domingo in northwestern Argentina had been for long considered as Upper Triassic-Lower Jurassic in age based on weak radiometric and paleontological evidence. Preliminary paleomagnetic data confirmed the age and opened questions about the nature of fossil footprints with avian features discovered in the section. Recently the stratigraphic scheme was reviewed with the identification of previously unrecognized discontinuities, and a radiometric dating obtained in a tuff, indicated an Eocene age for the Laguna Brava Formation and the fossil bird footprints, much younger than the previously assigned. We present a detailed paleomagnetic study interpreted within a regional tectonic and stratigraphic framework, looking for an explanation for the misinterpretation of the preliminary paleomagnetic data. The characteristic remanent magnetizations pass a tilt test and a reversal test. The main magnetic carrier is interpreted to be low Ti titanomagnetites and to a lesser extent hematite. The characteristic remanent magnetization would be essentially detrital. The obtained paleomagnetic pole (PP) for the Laguna Brava Formation has the following geographic coordinates and statistical parameters: N = 29, Lon. = 184.5° E, Lat. = 75.0° S, A95 = 5.6° and K = 23.7. When this PP is compared with another one with similar age obtained in an undeformed area, a declination anomaly is recognized. This anomaly can be interpreted as Laguna Brava Formation belonging to a structural block that rotated about 16° clockwise along a vertical axis after about 34 Ma. This block rotation is consistent with the regional tectonic framework, and would have caused the fortuitous coincidence of the PP with Early Jurassic poles. According to the interpreted magnetostratigraphic correlation, the Laguna Brava Formation would have been deposited during the Late Eocene with a mean sedimentation rate of about 1.4 cm per thousand years, probably in relation to the onset of the Andean deformation.

Vizán, H.; Geuna, S.; Melchor, R.; Bellosi, E. S.; Lagorio, S. L.; Vásquez, C.; Japas, M. S.; Ré, G.; Do Campo, M.



Constraining Basin Geometry and Fault Kinematics on the Santo Tomas Segment of the Agua Blanca Fault Through a Combined Geophysical and Structural Study  

NASA Astrophysics Data System (ADS)

The Santo Tomas basin, located in northern Baja California, formed at a right step in the dextral Agua Blanca fault (ABF). The ABF extends for more than 120km east from Punta Banda, with an east-west strike, and represents the southernmost fault in the San Andreas system of faulting. The basin is located roughly 40km south of Ensenada where the Agua Blanca fault intersects the Maximos fault. A detailed geophysical analysis defines the basin geometry, and helps to constrain the distribution and offset of mapped and concealed faults. Geophysical and structural data sets are combined to constrain the kinematic evolution of the Santo Tomas basin, including determining the relative amount of dip-slip and strike-slip motion on basin-bounding faults. Gravity data was collected over seven transects across and along the axis of the basin at 500 meter intervals, with 200 meter intervals at locations of known or inferred faults. Magnetic data were taken over the same lines, and are used in conjunction with gravity data to constrain the locations, geometries and displacements of intrabasinal faults. The combined gravity and magnetic data are modeled using Geosoft Oasis montaj software to create 2 3/4D models along profiles across the study area. Modeling of the geophysical data combined with structural mapping indicates that the Santo Tomas basin is bound by two major strike-slip faults, the ABF on the northeastern side and the Maximos fault on south, Based on offset markers, most of the strike-slip motion appears to be concentrated on the ABF on the north side of the basin. The ABF fault is characterized by multiple subparallel fault strands that appear to coalesce into single strands to the northwest and southeast of the basin. The Maximos is characterized by a single strand throughout the basin and it exhibits a minor dip-slip component. Basin sediments thicken slightly against the Maximos fault to as much as 1km. A third fault, cutting across the basin southeast of the town of Santo Tomas also exhibits a component of dip-slip motion. The total strike-slip offset on the two basin-bounding faults is inferred to be between 5 and 7 km (northern ABF) and <3 km (Maximos fault), for a combined ~9 km. This total is intermediate between the total slip on the ABF from studies to the southeast (22 km, Allen et al., 1960) and to the northwest (7 km, Callihan et al., 2008) suggesting that the fault is losing slip toward the northwest.

Springer, A.; Wetmore, P.; Fletcher, J.; Connor, C. B.; Callihan, S.; Beeson, J.; Wilson, J.



[Participation of the dog in the cycle of transmission of cutaneous leishmaniasis in the municipality of Viana, State of Espirito Santo, Brazil].  


In an endemic area of cutaneous leishmaniasis in the Viana municipality, Espírito Santo state, an investigation was performed on natural hosts of Leishmania among domestic animals, trying to correlate the presence of infected animals with the occurrence of the disease in humans. Within a three weeks period 186 dogs were examined and 32 (17.2%) were found infected. Eleven new cases of cutaneous leishmaniasis were recorded during one year, among people living in the endemic area. A close relationship was observed between the presence of infected dogs and the occurrence of human leishmaniasis. In the area studied, the disease seems to behave as a zoonosis maintained by domestic dogs. PMID:3586998

Falqueto, A; Coura, J R; Barros, G C; Grimaldi Filho, G; Sessa, P A; Carias, V R; dé Jesus, A C; de Alencar, J T



Inter-annual variations of macrobenthic communities over three decades in a land-locked coastal lagoon (Santo André, SW Portugal)  

NASA Astrophysics Data System (ADS)

Santo André is an enclosed brackish water coastal lagoon with temporary connections to the sea by a man-made channel. The exchange and mixture of saltwater and freshwater is irregular and the lagoon may show daily and seasonal fluctuations, but also long-term variation. Different benthic communities may be present along the annual cycle according to the magnitude of episodic freshwater and sea water inputs. In the last 30 years the communication with the sea has followed different regimes from year to year and, as a consequence, macrobenthic communities, assessed several times during the period before the opening to the sea, shifted from freshwater to marine affinities. Major differences were found between 1979 and 2010, with a preponderance of species with marine affinity, and the 1980s in which the organisms with freshwater affinity prevailed. Benthic communities are frequently used to assess aquatic environmental condition. Metrics used in the indices currently under discussion to assess ecological status of aquatic ecosystems within the scope of European Water Framework Directive were applied to Santo André data and the applicability of these metrics to assess quality in this coastal land-locked lagoon was discussed.

Correia, M. J.; Costa, J. L.; Chainho, P.; Félix, P. M.; Chaves, M. L.; Medeiros, J. P.; Silva, G.; Azeda, C.; Tavares, P.; Costa, A.; Costa, A. M.; Bernardo, J.; Cabral, H. N.; Costa, M. J.; Cancela da Fonseca, L.



Spatial distribution and concentration assessment of total petroleum hydrocarbons in the intertidal zone surface sediment of Todos os Santos Bay, Brazil.  


The primary objective of this study was to investigate the concentrations and spatial distribution of the total petroleum hydrocarbons (TPHs) in the intertidal zone surface sediment of Todos os Santos Bay, Brazil, to assess the distribution and degree of contamination by TPHs, measure the level of TPH degradation in the surface sediment, and identify the organic matter sources. The surface sediment used in this study was collected in 50 stations, and TPHs, isoprenoid alkanes (pristane and phytane), and unresolved complex mixture (UCM) were analyzed by gas chromatography with a flame ionization detector. The total concentrations ranged from 0.22 to 40,101 ?g g(-1) dry weight and showed a strong correlation with the total organic carbon (TOC) content. The highest TPH concentrations were observed in samples from the mangrove sediments of a river located near a petroleum refinery. Compared with other studies in the world, the TPH concentrations in the intertidal surface sediment of Todos os Santos Bay were below average in certain stations and above average in others. An analysis of the magnitude of UCM (0.11 to 17,323 ?g g(-1) dry weight) and the ratios nC17/Pr and nC18/Ph suggest that an advanced state of oil weathering, which indicates previous contamination. The molar C/N ratios varied between 5 and 43, which indicate organic matter with a mixed origin comprising marine and continental contributions. PMID:24100798

Silva, Carine S; Moreira, Icaro T A; de Oliveira, Olivia M C; Queiroz, Antonio F S; Garcia, Karina S; Falcão, Brunno A; Escobar, Narayana F C; Rios, Mariana Cruz



Tuning photochemical photophysical properties ruin(2 2'-bipyridine)2Lx complexes nonchromophoric ligand variations Ingle Simon D Lima Francisco C A Camilo Mariana R Daniel Julian F S Santos Evan D A Lima-Nett bandit S Carlos Rose M  

EPA Pesticide Factsheets

Did you mean: Tuning photochemical photophysical properties ruin(2 2'-bipyridine)2Lx complexes nonchromophoric ligand variations Ingle Simon D Lima Francisco C A Camilo Mariana R Daniel Julian F S Santos Evan D A Lima-Nett bandit S Carlos Rose M ?



Microsoft Academic Search

A long term (1989-1994) experiment with sugar cane (RB 739735) was carried out, in Linhares, ES, Brazil, in order to evaluate the effect of two systems of sugar cane harvesting: the first with previous burning (burned cane), and the second with crop residue remaining on the soil surface (green cane), on some physical properties of a tableland Yellow Podzolic soil.




Microsoft Academic Search

Sympathetic denervation has fallen into disfavour for operations. Dorso-perivertebral, paravertebral or retroperitoneal approaches are only of historic importance. The oblique retroperitoneal approach is popular because it provides good visibility, at the expense of a long skin incision. A videoscopic procedure has now been developed for lumbar sympathectomy. A transverse skin incision of 3–4cm is made in the hypochondrium, lateral to

J Wronski



[The Rondon Commission, diseases, and politics: "Região do Madeira: Santo Antônio," by Joaquim Augusto Tanajura - another view of the Alto Madeira in 1911].  


Physician Joaquim Augusto Tanajura (1878-1941), head of health services for the Rondon Commission from 1909 to 1912, wrote a paper about the first scientific journeys sponsored by the Oswaldo Cruz Institute. Entitled "Região do Madeira: Santo Antônio," it was published in the newspaper Jornal do Commercio de Manáos on June 5, 1911, at a time when rediscovery of Brazil's sertões had just begun and indictments were starting to be lodged about the state of abandonment and disease in which the interior of the country lay. Through Doctor Tanajura's article, the present text analyzes both the engagement of members of the Rondon Commission in local political dynamics and the era's controversies about construction of the image of the sertão and its inhabitants. PMID:21779698

Vital, André Vasques



[Malaria and hematological aspects among residents to be impacted by reservoirs for the Santo Antônio and Jirau Hydroelectric Power Stations, Rondônia State, Brazil].  


In Rondônia State, Brazil, two new hydroelectric plants, Santo Antônio and Jirau, are scheduled for construction on the Madeira River, upriver from the State capital, Porto Velho. The current study analyzes malaria prevalence before the construction and provides information on the possible impacts of malaria burden related to the influx of thousands of persons attracted by direct and indirect employment opportunities. According to the findings, malaria is present throughout the region, with varying prevalence rates. The existence of potential asymptomatic malaria carriers among the local population may be epidemiologically relevant and should be considered in the malaria control programs organized by public authorities and companies responsible for building the power plants, aimed at early diagnosis and treatment, vector control, water supply, and infrastructure in the urban areas. PMID:19578569

Katsuragawa, Tony Hiroshi; Cunha, Roberto Penna de Almeida; de Souza, Daniele Cristina Apoluceno; Gil, Luiz Herman Soares; Cruz, Rafael Bastos; Silva, Alexandre de Almeida E; Tada, Mauro Shugiro; da Silva, Luiz Hildebrando Pereira



Prevalence of potentially pathogenic free-living amoebae from Acanthamoeba and Naegleria genera in non-hospital, public, internal environments from the city of Santos, Brazil.  


Acanthamoeba and Naegleria species are free-living amoebae (FLA) found in a large variety of natural habitats. The prevalence of such amoebae was determined from dust samples taken from public non-hospital internal environments with good standards of cleanliness from two campuses of the same University in the city of Santos (SP), Brazil, and where young and apparently healthy people circulate. The frequency of free-living amoebae in both campuses was 39% and 17% respectively, with predominance of the genus Acanthamoeba. On the campus with a much larger number of circulating individuals, the observed frequency of free-living amoebae was 2.29 times larger (P< 0.00005). Two trophozoite forms of Naegleria fowleri, are the only species of this genus known to cause primary amoebian meningoencephalitis, a rare and non-opportunistic infection. We assume that the high frequency of these organisms in different internal locations represents some kind of public health risk. PMID:20464327

Teixeira, Lais Helena; Rocha, Silvana; Pinto, Rosa Maria Ferreiro; Caseiro, Marcos Montani; Costa, Sergio Olavo Pinto da



Molecular identification of Sporothrix species involved in the first familial outbreak of sporotrichosis in the state of Esp?rito Santo, southeastern Brazil  

PubMed Central

Sporotrichosis is a widespread subcutaneous mycosis caused by the dimorphic fungi now known as the Sporothrix schenckii complex. This complex is comprised of at least six species, including Sporothrix albicans, Sporothrix brasiliensis, Sporothrix globosa, Sporothrix luriei, Sporothrix mexicana and S. schenckii. Cases of sporotrichosis have significantly increased in Brazil over the past decade, especially in the state of Rio de Janeiro (RJ), where an epidemic among cat owners has been observed. The zoonotic transmission from cats to humans suggests a common source of infection and indicates that animals can act as vectors. We performed a molecular characterisation of samples collected during the first outbreak of familial sporotrichosis caused by S. brasiliensis in the state of Espírito Santo, Brazil. These results represent the first description of such an outbreak outside the endemic area of zoonotic sporotrichosis in RJ.

Oliveira, Manoel Marques Evangelista; Maifrede, Simone Bravim; Ribeiro, Mariceli Araujo; Zancope-Oliveira, Rosely Maria



Molecular identification of Sporothrix species involved in the first familial outbreak of sporotrichosis in the state of Espírito Santo, southeastern Brazil.  


Sporotrichosis is a widespread subcutaneous mycosis caused by the dimorphic fungi now known as the Sporothrix schenckii complex. This complex is comprised of at least six species, including Sporothrix albicans, Sporothrix brasiliensis, Sporothrix globosa, Sporothrix luriei, Sporothrix mexicana and S. schenckii. Cases of sporotrichosis have significantly increased in Brazil over the past decade, especially in the state of Rio de Janeiro (RJ), where an epidemic among cat owners has been observed. The zoonotic transmission from cats to humans suggests a common source of infection and indicates that animals can act as vectors. We performed a molecular characterisation of samples collected during the first outbreak of familial sporotrichosis caused by S. brasiliensis in the state of Espírito Santo, Brazil. These results represent the first description of such an outbreak outside the endemic area of zoonotic sporotrichosis in RJ. PMID:24141957

Oliveira, Manoel Marques Evangelista; Maifrede, Simone Bravim; Ribeiro, Mariceli Araújo; Zancope-Oliveira, Rosely Maria



Integration of the PSI technique and conventional ground-based methods for landslide characterization and monitoring: the case study of Santo Stefano d'Aveto; Northern Apennines, Italy  

NASA Astrophysics Data System (ADS)

Santo Stefano village is located in the Northern Apennines (Italy) and is built up on an ancient landslide, defined as a complex phenomenon that is an earth rotational slide evolving into a flow. The landslide has an extension of 1,3 km2 and a volume of about 10 million of m3. The landslide can be defined as active and according to Cruden & Varnes (1996) nomenclature the velocity range from very slow to extremely slow. The landslide poses and high risk to the buildings and infrastructures causing extensive direct damages. The Santo Stefano d'Aveto village is located inside a glacial valley made mainly of ophliotic rocks, sandstones and marls and filled with glacial deposits. The landslide occurred in the glacial deposits composed of debris in a sandy-clayey matrix. Santo Stefano d'Aveto landslide has been monitored through radar interferometry, in particular with the PS-InSAR technique (Ferretti et al., 2001) and through ground-based instruments such as inclinometers and piezometers. The PS-InSAR analysis has been performed using ascending SAR scenes and descending SAR scenes from ERS-1/-2 (1992-2001), and ascending and descending SAR scenes from ENVISAT (2002-2008). All the datasets have been processed in the advanced mode APSA that means for each PS has been provided deformation time series relative to a reference date (zero). The target points within the Santo Stefano landslide have a high density. In general for all the analysed datasets it has been observed a decrease of velocity from upslope portion to the downslope one of the landslide. The maximum velocity recorded in the ERS dataset is around 38 mm/y, while the maximum velocity recorded in the ENVISAT dataset is around 20 mm/y. The APSA analysis has provided information about the temporal evolution of target points. Both the majority of ERS and ENVISAT time series have shown a seasonal trend related to the variation of the water table level, which rises during rainfall season and decreases during dry season. The time series analysis show that the landslide deformation pattern is strongly controlled by the pore water pressure condition as demonstrated by the piezometric measurements. As usual an increase of water pressure condition led to a decrease of shear strength of the soil causing an increase of movement. Six inclinometric instruments have been installed in 2001 and measurements have been acquired until 2006. A comparison between the deformation measured by the PS techniques and the inclinometric acquisitions has been performed examining the velocity measurements along the same line of sight. In particular in order to perform the comparison, the displacements of the inclinometers, taken along the maximum slope angle direction have been plotted along the line of sight of the satellite. The results show that the displacements measured by the two different type of techniques are consistent. The PSI analysis performed has contributed to a more in depth investigation of the phenomenon, in particular the PS measurements and geomorphologic interpretation have allowed to redefine better the boundaries of landslide and the state of activity while the time series analysis has allowed to better understand the deformation pattern and its relations with the causes of the landslide itself. Once the relationship between actual displacements vectors (measured by in-situ instrumenation) and l.o.s. components is known, the APSA analysis can provide a much larger dataset on ground movement allowing a better modelling of the surface displacements field.

Tofani, Veronica; Pancioli, Valeria; Catani, Filippo; Casagli, Nicola



Overview of the 20th century impact of trace metal contamination in the estuaries of Todos os Santos Bay: past, present and future scenarios.  


This paper discusses the distribution patterns of trace metals in sediments and suspended particulate matter (SPM) in the three main estuaries of the Todos os Santos Bay (BTS), Brazil, during dry and rainy seasons. Data available up to 2012 was also reviewed to assess the status of contamination. For most elements, metal concentrations in sediments decreased from the tidal limits to the lower estuary. Metals in SPM presented more complex distributions along the salinity gradient. Metal variability between rainy and dry conditions was only significant for SPM data. Of the BTS estuaries, the levels of Cd, Zn, Pb and Cu are highest in the Subaé estuary, and they seem to be promoting harmful biological effects in macrofauna, and also may pose potential human health risks. Despite the evidence of important localized contamination, much of the data compiled indicates that the bay and its estuaries are still relatively preserved. PMID:22841387

Hatje, Vanessa; Barros, Francisco



Quality of life in leprosy: a comparative analysis between patients in the Amazon region and patients in Santo André in the ABC region of São Paulo, Brazil.  


This study analyzed the quality of life of individuals with leprosy and compared quality of life indexes of patients in two different socioeconomic scenarios. The study was conducted at the leprosy clinic of the ABC School of Medicine, São Paulo, Brazil and during visits to the populations living along the Purus River in the Brazilian state of Amazonas. The Dermatology Life Quality Index (DLQI) was used to evaluate the patients. Quality of life was found to be impaired in 76.9% of the patients evaluated in the Amazon compared to 19% of the patients in Santo André. In the group of patients in the Amazon who had sequelae of the disease, quality of life was impaired. PMID:21308328

Proto, Rodrigo Sestito; Machado Filho, Carlos D'Apparecida Santos; Rehder, José Ricardo Carvalho Lima; Paixão, Maurício Pedreira; Angelucci, Rodrigo Interlandi




Microsoft Academic Search

Dialogue among cultures is a main characteristic on the knowledge construction in the 21st century societies. In this document, following a tradition started around thirty years ago, we try a conversation between western science and eastern Mahayana Buddhist school. The goal of this conversation is to find what contribute this philosophical tradition may give to the development of sustained societies

Orlando Figueiredo; Escola Secundária de Peniche



Evaluación y perspectivas del Código Nacional de Recursos Naturales de Colombia en sus 30 años de vigencia  

Microsoft Academic Search

Los artículos presentados estudian la eficacia, vigencia y perspectivas del Código Nacional de Recursos Naturales, decreto 2811 de 1974, teniendo de presente el avanzado estado de la ciencia en Colombia establecido por la Constitución del 91, y la Ley 99 de 1993; de esta manera los doctrinantes retoman la historia y las funciones del Código, la biodiversidad, la diversidad de

Autores varios


Pregadoras murshidat como agentes de mudança no Marrocos: uma perspectiva comparativa  

Microsoft Academic Search

Resumo As feministas marroquinas tem como objetivo promover posições de poder para as mulheres através da educação, da auto- consciência e do conhecimento de novos direitos legais; e também disseminando informações a respeito do novo código da família e do novo código trabalhista por meio de suas ONGs e grupos comunitários. O ativismo das mulheres contribuiu de maneira significativa para

Moha Ennaji



Perspectivas Pedagogicas. Documentos-Ponencias Y Ensayos Proyecto Teacher Corps, Ciclo XII (Pedagogical Perspectives. Documents, Papers and Essays, Teacher Corps Project, Cycle XII).  

ERIC Educational Resources Information Center

The purpose of this book is to stimulate ideas leading to a sharing of approaches, strategies, and methodologies applicable to the education of Puerto Ricans. Following introductory material, 18 papers are presented, the first 10 of which are in Spanish. Titles and authors are: (1) "El maestro que Puerto Rico necesita," Leonides Santos Vargas; (2)…

Albino, Isidra, Ed.; Davila, Sonia, Ed.


Endemic and threatened tetrapods in the restingas of the biodiversity corridors of Serra do Mar and of the Central da Mata Atlântica in eastern Brazil.  


Biodiversity corridors comprise a mosaic of land uses connecting fragments of natural forest across a landscape. Two such corridors have been established along the eastern coast of Brazil: the Serra do Mar and the Central da Mata Atlântica corridors, along which most of the coastal plains are restinga areas. In this study, we analyze the present status of the endemic and endangered terrestrial vertebrates of both corridors. We sampled 10 restingas in both corridors, recording species of amphibians, reptiles, birds, and mammals. Some restingas harbor a relatively large number of endemic species,and two main regions of endemism can be identified along the restingas of both corridors: the coastal restingas from northern Espirito Santo State to southern Bahia State (between Linhares, ES, and Tarancoso, BA), and the coastal region between the restingas of Maricá and Jurubatiba, Rio de Janeiro State. Six species of terrestrial vertebrates considered threatened with extinction are found in the restingas of Serra do Mar and Central da Mata Atlântica biodiversity corridors (Liolaemus lutzae, Formicivora littoralis, Mimus gilvus, Schistochlamys melanopis, and Trinomys eliasi). The region located between the restinga of Maricá and that of Jurubatiba is of special relevance for the conservation of vertebrate species of the restingas of the corridors because a considerable number of threatened species of terrestrial vertebrates are found there. We strongly recommend efforts to develop checklists of threatened faunas for the States of Espirito Santo and Bahia. PMID:16025914

Rocha, C F D; Van Sluys, M; Bergallo, H G; Alves, M A S



Migratory flows and foraging habitat selection by shorebirds along the northeastern coast of Brazil: The case of Baía de Todos os Santos  

NASA Astrophysics Data System (ADS)

Large numbers of Nearctic shorebirds migrate and winter along the coast of northeastern Brazil, but there is little information on their migratory flows, foraging ecology, and on the structure of the species assemblages that they form with resident shorebirds. We studied these issues on intertidal flats of Baía de Todos os Santos (Bahia), the second largest bay in Brazil. During a full year cycle we carried out weekly bird counts in an intertidal area of 280 ha divided in sectors, where we also measured environmental parameters. The analyses of weekly counts resulted in a detailed phenology of use of the area by shorebirds. Five species were resident and ten were Nearctic migrants. Several of the latter had clear peaks in numbers in March and October, revealing the use of the bay as a stopover during both the north-bound and south-bound migration flows. A canonical correspondence analysis of the relationship between environmental parameters and bird numbers indicated that the foraging bird assemblage could be divided into five main groups, occupying distinct ecological gradients in the study area. The most important factors driving this structure were invertebrate prey abundance, percentage of fine sediments, area of mangrove cover and distance to channels. Our findings imply that maintenance of the diversity of intertidal habitats in this bay is crucial to satisfy the particular habitat requirements of resident and migrant shorebirds using the northeastern coastal regions of Brazil.

Lunardi, Vitor O.; Macedo, Regina H.; Granadeiro, José P.; Palmeirim, Jorge M.



Evaluation of optimal conditions for determination of low selenium content in shellfish samples collected at Todos os Santos Bay, Bahia, Brazil using HG-AFS.  


This work proposes a procedure for the determination of total selenium content in shellfish after digestion of samples in block using cold finger system and detection using atomic fluorescent spectrometry coupled hydride generation (HG AFS). The optimal conditions for HG such as effect and volume of prereduction KBr 10 % (m/v) (1.0 and 2.0 ml) and concentration of hydrochloric acid (3.0 and 6.0 mol L(-1)) were evaluated. The best results were obtained using 3 mL of HCl (6 mol L(-1)) and 1 mL of KBr 10 % (m/v), followed by 30 min of prereduction for the volume of 1 mL of the digested sample. The precision and accuracy were assessed by the analysis of the Certified Reference Material NIST 1566b. Under the optimized conditions, the detection and quantification limits were 6.06 and 21.21 ?g kg(-1), respectively. The developed method was applied to samples of shellfish (oysters, clams, and mussels) collected at Todos os Santos Bay, Bahia, Brazil. Selenium concentrations ranged from 0.23?±?0.02 to 3.70?±?0.27 mg kg(-1) for Mytella guyanensis and Anomalocardia brasiliana, respectively. The developed method proved to be accurate, precise, cheap, fast, and could be used for monitoring Se in shellfish samples. PMID:24771464

Lopes Dos Santos, Walter Nei; Macedo, Samuel Marques; Teixeira da Rocha, Sofia Negreiros; Souza de Jesus, Caio Niela; Cavalcante, Dannuza Dias; Hatje, Vanessa



Structural controls on a carbon dioxide-driven mud volcano field in the Northern Apennines (Pieve Santo Stefano, Italy): Relations with pre-existing steep discontinuities and seismicity  

NASA Astrophysics Data System (ADS)

The strong release of carbon dioxide (CO 2)-rich fluids in the axial zone of the Northern Apennines, near Pieve Santo Stefano, is analysed in relation to both structural controls and the microseismicity that followed the earthquake of 26 November 2001. Some vents recorded a transition from gas-dominated emission to post-seismic mud extrusion. The co-seismic rupture of a portion of the NW-trending Alto Tiberina Fault, which bounds the Quaternary Upper Tiber Basin, presumably broke the hydraulic seal trapping overpressured CO 2-rich fluid compartments and released a fluid pressure pulse. Fault pattern analysis has revealed that venting localises along NE-trending steep faults associated with the regional transverse Arbia-Val Marecchia Line bounding the basin to the northwest. It is proposed that the transverse faults have been acting as permeable pathways allowing the post-seismic transport of overpressured fluids to the surface, during which the mud was mobilised. A hypothetical correlation is also established between failure mechanisms and the 2001 aftershocks. Results of Mohr constructions indicate that frictional fault reactivation is a suitable mechanism for explaining the seismic events along the NW-trending normal fault, while hydraulic fracturing is the most suitable to describe the seismicity occurring along the transverse faults.

Bonini, Marco



Survey of Trypanosoma and Leishmania in wild and domestic animals in an Atlantic rainforest fragment and surroundings in the state of Espírito Santo, Brazil.  


Trypanosoma and Leishmania infections affect wild and domestic animals and human populations. The growing process of deforestation and urbanization of Atlantic Rainforest areas has given rise to introduction of humans and domestic animals to the sylvatic cycles of Trypanosoma and Leishmania species. Serological, parasitological, and molecular surveys among wild and domestic animals in the Corrego do Veado Biological Reserve, which is an Atlantic Rainforest fragment in the state of Espírito Santo, southeastern Brazil, were evaluated. In total, 154 wild animals of 25 species and 67 domestic animals (47 dogs and 20 horses) were sampled. All the domestic animals were serologically negative for anti-Leishmania infantum chagasi antibodies and negative in parasitological approaches. Only the Order Chiroptera presented positive blood cultures and cryopreserved isolates. The phylogenetic trees based on SSU rDNA and gGAPDH genes confirmed the occurrence of Trypanosoma dionisii and provided the first record of Trypanosoma cruzi marinkellei in southeastern Brazil. The studies conducted in Atlantic Rainforest remaining trees provide the knowledge of parasite diversity or detect parasites that can accelerate the loss of hosts diversity. PMID:24897863

Acosta, Igor da C L; Da Costa, Andrea P; Gennari, Solange M; Marcili, Arlei



[The prevalence of anemia and its association with socio-demographic and anthropometric aspects in children living in Vitória, State of Espírito Santo, Brazil].  


The high prevalence of iron deficiency in the population is widely acknowledged, but there are not yet sufficient studies that explain the reasons for this problem at local and national level. Thus, the scope of this study was to evaluate the prevalence of anemia and its relation with socio-demographic and anthropometric aspects of children living in Vitoria, Espírito Santo. This is a cross-sectional study conducted among 12- to 72-month-old children. Data were collected in Primary Healthcare Units and involved the hemoglobin dosage, height and weight measurement, in addition to the application of a semi-structured questionnaire to gather socio-demographic information. The evaluation included 980 children, 51.1% of which were female with a median age of 46.00 (95%CI: 45.52 - 48.09) months. Anemia was found in 37%, with a hemoglobin median of 11.4 (95%CI: 11.15 - 11.36) g/dL. The children's' height/age ratio (p = 0.049) and the number of people in the family (p < 0.001) were found to be determinants of anemia, and age was a factor of protection for this deficiency (p = 0.010). The high prevalence of anemia and its strong relation with demographic and anthropometric data suggest the need for more effective public policies than just food fortification and supplementation. PMID:24196892

de Oliveira, Ana Paula Della Nina; Pascoal, Mariana Nunes; dos Santos, Luana Caroline; Pereira, Simone Cardoso Lisboa; Justino, Liusani Elizia Honório; Petarli, Glenda Blaser; Kitoko, Pedro Makumbundu



Spatial variability of trace metals and inorganic nutrients in surface waters of Todos Santos Bay, México in the summer of 2005 during a red tide algal bloom.  


Dissolved and particulate metals (Ag, Cd, Co, Cu, Ni, and Zn) and nutrients (PO(4), NO(3), and H(4)SiO(4)) were measured in Todos Santos Bay (TSB) in August 2005. Two sources producing local gradients were identified: one from a dredge discharge area (DDA) and another south of the port and a creek. The average concentrations of dissolved Cd and Zn (1.3 and 15.6 nM, respectively) were higher by one order of magnitude than the surrounding Pacific waters, even during upwelling, and it is attributed to the presence of a widespread and long-lasting red tide coupled with some degree of local pollution. A clear spatial gradient (10 to 6 pM), from coast to offshore, of dissolved Ag was evident, indicating the influence of anthropogenic inputs. The particulate fraction of all metals, except Cu, showed a factor of ~3 decrease in concentrations from the DDA to the interior of the bay. The metal distributions were related to the bay's circulation by means of a numerical model that shows a basically surface-wind-driven offshore current with subsurface compensation currents toward the coast. Additionally, the model shows strong vertical currents over the DDA. Principal component analysis revealed three possible processes that could be influencing the metal concentrations within TSB: anthropogenic inputs (Cd, Ag, and Co), biological proceses (NO(3), Zn, and Cu), and upwelling and mixing (PO(4), H(4)SiO(4), Cd, and Ni). The most striking finding of this study was the extremely high Cd concentrations, which have been only reported in highly contaminated areas. As there was a strong red tide, it is hypothesized that the dinoflagellates are assimilating the Cd, which is rapidly remineralized and being concentrated on the stratified surface layers. PMID:18726538

Lares, M L; Marinone, S G; Rivera-Duarte, I; Beck, A; Sañudo-Wilhelmy, S



Probing The Buried Remains of The Todos los Santos, City of San Salvador in Hoping Island with Shallow Subsurface Geophysics Method  

NASA Astrophysics Data System (ADS)

The study in ancient sites with GPR is widely documented over several decades. This non-invasive geophysical method provides a rapid measure for anthropogenic objects and therefore serves as a guide for possible excavation for the next stage of archaeological surveys. City of San Salvador, which is a Dutch colonial city consisted of fortress, hospitals and churches in 17 century, is located in the Hoping Island in Keelung, Taiwan. The fortress and its affiliated structures were abandoned and left collapsing since the mid-17th century. Some relics of the fortress wall were still remained until the early 20th century but the fast development projects in the island has caused the relics demolished or buried under building or road pavements. Many wells and bones have been found around the area belong to over three hundred years ago. As a consequence, the government initiated a new excavation project at the parking lot where the ancient convent of Todos los Santos is believed since 2011 in order to find the remains of the convent in city of San Salvador. Meanwhile we have surveyed with GPR to help guiding the excavation location. In this case, we surveyed with wide-angle-refraction/reflection (WARR) of GPR as well as common-offset array, to compensate the defect of traditional common-offset of lack of longitudinal resolution with velocity profile, and the combination of velocity profile and common-offset data helped distinguish the signals from other noises and further located the position of subsurface structures. After data analysis and numerical modeling of the buried materials, we have located the possible remains of walls of the convent or other structures around 0.9 to 1.3 meters in depth that can offer useful information to better plan the archaeological excavations.

Tsai, Yi-fan; Chang, Pin-yu; Eugenio Borao Mateo, José



Origin of step-like and lobate seafloor features along the continental shelf off Rio de Janeiro State, Santos basin-Brazil  

NASA Astrophysics Data System (ADS)

A combined analysis of seismic and morphological features identified in a set of high-resolution seismic reflection and bathymetric data, shows a systematic relationship between major modern seafloor morphological traces and the basinward migration of Late Pleistocene coastlines along the continental shelf of the Santos basin (Rio de Janeiro State, SE Brazil). Observed fairly continuous and sinuous mid-outer shelf escarpments are related to the sea-level variations and shelf exposure during the Last Glacial cycle. A bathymetric step at - 110 m is an erosional remnant of offlapping detached forced-regressive wedges that spread over 50 km in the shelf-dip direction, probably developed during periods of falling sea level between MIS 3 and 2. A second major escarpment at - 130 m was interpreted as the shoreline during the LGM, at the time of most extensive subaerial exposure of the continental shelf. However, a distal escarpment at - 150 m is expressed as a straight contour feature along the two main shelf-edge embayments that characterize the shelf break. This escarpment is coupled with a basal seaward-inclined and highly eroded ramp, and was interpreted as the erosional action of bottom currents during the last transgression due to the displacement of the southward flowing Brazil Current towards the present-day outer shelf. Previously published articles have regarded the morphological features observed on the modern shelf as indicators of stillstands during the post-LGM transgression. We conclude that, on the contrary, most of these features are actually from earlier parts of the Late Pleistocene and were formed in a regressive scenario under oscillating and relative slow sea-level fall.

Reis, A. T.; Maia, R. M. C.; Silva, C. G.; Rabineau, M.; Guerra, J. V.; Gorini, C.; Ayres, A.; Arantes-Oliveira, R.; Benabdellouahed, M.; Simões, I.; Tardin, R.



Transmitted Drug Resistance Among Antiretroviral-Naive Patients with Established HIV Type 1 Infection in Santo Domingo, Dominican Republic and Review of the Latin American and Caribbean Literature  

PubMed Central

Abstract Emergence of HIV resistance is a concerning consequence of global scale-up of antiretroviral therapy (ART). To date, there is no published information about HIV resistance from the Dominican Republic. The study's aim was to determine the prevalence of transmitted drug resistance (TDR) to reverse transcriptase and protease inhibitors in a sample of chronically HIV-1-infected patients in one clinic in Santo Domingo. The data are presented in the context of a review of the TDR literature from Latin America and the Caribbean. Genotype testing was successfully performed on 103 treatment-naive adults planning to initiate antiretroviral therapy; the World Health Organization (WHO) list of surveillance drug resistance mutations (SDRM) was used to determine the presence of TDR mutations. WHO SDRM were identified in eight patients (7.8%); none had received sdNVP. There were no significant differences in epidemiologic or clinical variables between those with or without WHO SDRM. The prevalence of WHO SDRM was 1.0% and 6.8% for nucleoside reverse transcriptase inhibitors and nonnucleoside reverse transcriptase inhibitors, respectively. No WHO SDRMs for protease inhibitors were identified. Among 12 studies of TDR in the region with a sample size of at least 100 subjects, the reported prevalence of SDRM ranged from 2.8% to 8.1%. The most commonly identified SDRM was K103N. This information adds to our understanding of the epidemiology of TDR in the region and the possible role such mutations could play in undermining first-line treatment. Ongoing surveillance is clearly needed to better understand the TDR phenomenon in the Caribbean.

Taylor, Barbara S.; Rojas Fermin, Rita A.; Reyes, Emily Virginia; Vaughan, Catherine; Jose, Lina; Javier, Carmen; Franco Estevez, Ramona; Donastorg Cabral, Yeycy; Batista, Arelis; Lie, Yolanda; Coakley, Eoin; Hammer, Scott M.; Brudney, Karen



Transmitted drug resistance among antiretroviral-naive patients with established HIV type 1 infection in Santo Domingo, Dominican Republic and review of the Latin American and Caribbean literature.  


Emergence of HIV resistance is a concerning consequence of global scale-up of antiretroviral therapy (ART). To date, there is no published information about HIV resistance from the Dominican Republic. The study's aim was to determine the prevalence of transmitted drug resistance (TDR) to reverse transcriptase and protease inhibitors in a sample of chronically HIV-1-infected patients in one clinic in Santo Domingo. The data are presented in the context of a review of the TDR literature from Latin America and the Caribbean. Genotype testing was successfully performed on 103 treatment-naive adults planning to initiate antiretroviral therapy; the World Health Organization (WHO) list of surveillance drug resistance mutations (SDRM) was used to determine the presence of TDR mutations. WHO SDRM were identified in eight patients (7.8%); none had received sdNVP. There were no significant differences in epidemiologic or clinical variables between those with or without WHO SDRM. The prevalence of WHO SDRM was 1.0% and 6.8% for nucleoside reverse transcriptase inhibitors and nonnucleoside reverse transcriptase inhibitors, respectively. No WHO SDRMs for protease inhibitors were identified. Among 12 studies of TDR in the region with a sample size of at least 100 subjects, the reported prevalence of SDRM ranged from 2.8% to 8.1%. The most commonly identified SDRM was K103N. This information adds to our understanding of the epidemiology of TDR in the region and the possible role such mutations could play in undermining first-line treatment. Ongoing surveillance is clearly needed to better understand the TDR phenomenon in the Caribbean. PMID:21851324

Myers, Julie E; Taylor, Barbara S; Rojas Fermín, Rita A; Reyes, Emily Virginia; Vaughan, Catherine; José, Lina; Javier, Carmen; Franco Estévez, Ramona; Donastorg Cabral, Yeycy; Batista, Arelis; Lie, Yolanda; Coakley, Eoin; Hammer, Scott M; Brudney, Karen




Microsoft Academic Search

A tecnologia está mais acessível ao produtor. O valor de um computador hoje equivale a 1 tonelada de adubo (uréia). A medida que o custo da tecnologia caiu, os valores dos fatores de produção – fertilizantes e salários – subiram significamente. Assim, é vital o produtor encontrar formas de otimizar o uso de insumos e melhorar a produtividade da mão-de-obra

Margarete Boteon; Rafaela Cristina Da Silva; Joao Paulo Bernardes Deleo



Petrography, geochemistry, and depositional setting of the San Pedro and Santo Tomas coal zones: anomalous algae-rich coals in the middle part of the Claiborne Group (Eocene) of Webb County, Texas  

USGS Publications Warehouse

Two coal zones, the San Pedro and the overlying Santo Tomas, are presented for nearly 35 km in outcrop, surface and underground mines, and shallow drill holes along the strike of the middle part of the Claiborne Group (Eocene) in Webb County, Texas. A sandstone-dominated interval of 25 to 35 m separates the two coal zones, which range up to 3 m in thickness. The coal-bearing portion of the middle Claiborne Group in the Rio Grande area represents a fining-upward transition from sandstone-dominated, marine-influenced, lower delta plain depositional environments to more inland, mudstone-rich, predominantly freshwater deltaic settings. The less variable nature of the Santo Tomas coal zone reflects its origin in the upper part of an interlobe basin that received only minor clastic influx. Petrographic attributes of the nonbanded coals indicate that they formed subaqueously in fresh to possibly brackish waters. Petrographic study of polished blocks indicates that approximately 10% of the nonbanded coal from both coal zones is composed of green algae fructifications. -from Authors

Warwick, P. D.; Hook, R. W.



Comment on “Structural controls on a carbon dioxide-driven mud volcano field in the Northern Apennines (Pieve Santo Stefano, Italy): Relations with pre-existing steep discontinuities and seismicity”  

NASA Astrophysics Data System (ADS)

Bonini (2009, Structural controls on a carbon dioxide-driven mud volcano field in the Northern Apennines (Pieve Santo Stefano, Italy): relations with pre-existing steep discontinuities and seismicity. Journal of Structural Geology 31, 44-54) presents a 2D mechanical analysis to infer the failure conditions responsible for the seismicity distribution during an M w = 4.6 seismic sequence nucleating during 2001 in the Northern Apennines. In my view the mechanical analysis presented in this paper has some weakness or is not well constrained, in particular: 1) the assumption of a dip angle of 50°, is not consistent with the activated structures; 2) the P f = ?3 condition, difficult to be attained along a cohesionless fault dipping at 50°; 3) the isotropic stress state, i.e. ?2 = ?3, that is not consistent with the active or recent stress field in the area.

Collettini, Cristiano



[Occurrence of a hepatitis A outbreak in three neighborhoods of the city of Vitória, Espírito Santo State, and its relation with the quality of the water for human consumption].  


This work had as purpose to analyze the possible factors related with the occurrences of Hepatitis in the neighborhoods of Grande Vitória, Estrelinha and Inhanguetá located in the city of Vitória, State of the Espírito Santo, from April the June, 2006, and the quality of the water consumed by the population. The indicators for the water quality used were the Free Residual Chlorine concentrations (FRC) and the presence of thermotolerant coliforms. The microbiological analyses performed in the water consumed for the studied communities did not presented results indicating contamination by thermotolerants coliforms and the percentage of FRC determined between January and June were always above of the minimum limit demanded by the legislation (Act 518/2004). The results showed that the occurrence of a Hepatitis A outbreak can be related with the absence of physical barriers that impede the contact of people with the contaminated water and the practical deficiencies in the hygienic practices adopted, individually, by the local population. PMID:20069184

Silva, Lindalva Marques da; Souza, Edna Hollunder de; Arrebola, Tânia Maria; Jesus, Gabriel Dos Anjos de




Microsoft Academic Search

Considerando-se que existe um déficit na capacidade estática de armazenamento de grãos no Brasil, aliado a baixa margem de rentabilidade auferida pelos produtores de commodities agrícolas, notadamente a soja, bem como o fato de que a armazenagem em nível de propriedade rural pode vir a se constituir em um diferencial positivo ao produtor no momento da comercialização desta oleaginosa, buscou-se

Alberto Silva Dutra; Joao Armando Dessimon Machado; Regis Rathmann



Evolução recente do Ensino Secundário em Portugal e suas implicações nos currículos de Geologia; a perspectiva da Associação Portuguesa de Geólogos  

Microsoft Academic Search

Being aware of the Science Education\\/Training significance, the APG followed with concern the successive cur - ricular reorganizations experienced by the Portuguese Secondary School level in the last few years. The general structure of the current learning programmes is not completely unfavourable to Geology, recognising its educa- tional and cultural value. There are, however, various problems that must be solved

Edite Bolacha; António Mateus; D. Dinis; Rua Manuel Teixeira Gomes



[Evaluation of the quality of data in the Live Birth Information System and the Information System on Mortality during the neonatal period in the state of Espírito Santo, Brazil, between 2007 and 2009].  


Health information systems make it possible to be aware of health problems and often represent the sole source of information, thereby making it essential to assess their quality. The scope of this paper was to evaluate the quality of data about live births and deaths in the neonatal period in the Live Births Information System (SINASC) and Mortality Information System (SIM) in the state of Espírito Santo during the period from 2007 to 2009. Descriptive methodology with secondary data obtained from the electronic address of the Informatics Department of the Unified Health System was used to analyze the dimensions of quality of information, namely accessibility, timeliness and completeness. The results indicate that the information is accessible and timely, with a lag of three years compared to the beginning of the study. The completeness of the variables analyzed in SINASC was predominantly classified as excellent. SIM revealed a high percentage of missing data on the number of the Live Birth Declaration, mother's education, age and reproductive history. The conclusion reached is that SINASC is an excellent source of information about births, though SIM reveals a marked need for improvements in the completeness of its information. PMID:25014281

Silva, Laura Pedroza da; Moreira, Claudia Maria Marques; Amorim, Maria Helena Costa; Castro, Denise Silveira de; Zandonade, Eliana



[Analysis of motor performance associated with the nutritional status of the elderly enrolled in the Family Health Program in the municipality of Vitória de Santo Antão in the State of Pernambuco].  


The scope of this paper was to investigate the relationship between the nutritional status and the motor skills of the elderly registered in the Family Health Program in the urban area of Vitória de Santo Antão in the State of Pernambuco, by means of a transversal epistemological descriptive-analytical field study. The participants of this survey were 235 elders, both male and female of 60-years-old and over registered in the family health program in the urban area of the city. The nutritional status was evaluated by the body mass index and the circumference of the calf. Four motor tests were used to evaluate motor performance: Time Up&Go, Tinetti's Index, Functional Reach and Unipodal Support. The associations were checked using Spearman's correlation. The association of the nutritional status with the motor tests was statistically significant among males* or females**: (Time Up&Go rho = 0.290* / 350**; Functional Reach rho = 0.232* / 352**; Tinetti's Index rho = 243* / 363** and Unipodal Support rho = 221* / 475**. The evaluation of the results revealed an association between nutritional status and functional capacity of the elders no matter what gender; with obesity being a limiting nutritional condition to adequate performance in the tests. PMID:22634822

Soares, Lisandra Delfino de Albuquerque; Campos, Florisbela de Arruda Câmara e Siqueira; Araújo, Maria das Graças Rodrigues de; Falcão, Ana Patrícia Siqueira Tavares; Lima, Bruna Rafaela Dornelas de Andrade; Siqueira, Danielle Ferreira de; Fittipaldi, Etiene Oliveira da Silva; Arruda, Silvana Gonçalves Brito de; Faro, Zelyta Pinheiro de




Microsoft Academic Search

Foi o trabalho sistemático com a técnica da análise do caráter que levou Reich a descobrir que os distúrbios psico-emocionais apresentam como equivalente somático, a couraça muscular. Couraças são tensões que se formam ao longo da vida, cuja função é proteger o indivíduo (ego) de experiências dolorosas e ameaçadoras. Inclui tensão ou flacidez crônicas da musculatura esquelética que são resultados

José Henrique Volpi; Maria Beatriz de Paula


O Debate sobre a Homossexualidade Mediado por Representações Sociais: Perspectivas Homossexuais e Heterossexuais The Debate on Homosexuality Mediated by Social Representations: Homosexual and Heterosexual Perspectives  

Microsoft Academic Search

Resumo O objetivo deste trabalho foi estudar as representações sociais da homossexualidade entre estudantes universitários, segundo orientação sexual e sexo. Foram aplicadas uma questão de associação livre sobre a palavra homossexualidade e outra sobre as possíveis causas da mesma, sendo os dados analisados segundo os princípios da análise de conteúdo. Houve diferenças significativas entre os grupos, possibilitando-nos encontrar diferentes maneiras

Anderson Scardua; Edson Alves de Souza



Microsoft Academic Search

Na generalidade dos países desenvolvidos, os sistemas públicos de pensionamento têm-se confron- tado com o aumento da longevidade e com o decréscimo da natalidade das suas populações. Quase invariavelmente, a reacção dos decisores políticos traduziu-se no aumento da idade legal da reforma, isto é, da idade em que os trabalhadores são investidos no direito a receber uma pensão completa; na

Pedro Martins; Álvaro Novo; Pedro Portugal



Berengario da Carpi.  


Berengario da Carpi was magister of anatomy and surgery at the University of Bologna from 1502 to 1527. Eustachio and Falloppia defined him as 'the restaurator of anatomy'. He was a great surgeon, anatomist and physician of illustrious patients including Lorenzo II dei Medici, Giovanni dalle Bande Nere, Galeazzo Pallavicini, Cardinal Colonna, and Alessandro Soderini. He had strong links to the intellectuals of his time (Forni, Bonamici, Manuzio, Pomponazzi) as well as with the Medici family. He was respected by the Popes Julius II, Leo X and Clement VII. His main contributions are the Isogogae Breves, De Fractura calvae sive cranei, and the illustrated Commentaria on the Anatomy of Mondino de Liucci, a textbook utilized for more than 200 years, which Berengario aimed to restore to its initial text. The Commentaria constitutes the material for the last part of this paper which concludes with a personal translation of some passages on 'The kidney', where the author gives poignant examples of experimental ingenuity. PMID:10213820

De Santo, N G; Bisaccia, C; De Santo, L S; De Santo, R M; Di Leo, V A; Papalia, T; Cirillo, M; Touwaide, A



Detection of lamivudine-resistant variants and mutations related to reduced antigenicity of HBsAg in individuals from the cities of Santos and S?o Paulo, Brazil  

PubMed Central

Background Continuous long-term treatment is recommended to reduce the hepatitis B virus (HBV) viral load. However, as a consequence, resistance mutations can emerge and be transmitted to other individuals. The polymerase (POL) gene overlaps the surface (S) gene. Thus, during treatment, mutations in the POL gene may lead to changes in hepatitis B surface antigen (HBsAg). The purpose of this study was to evaluate the frequency of lamivudine and vaccine escape mutations in HBsAg-positive blood donors from the city of Santos and in untreated HBV mono-infected patients from the city of São Paulo, Brazil. Methods HBV DNA was extracted from 80 serum samples, of which 61 were from volunteer blood donors and 19 were from untreated HBV patients. A fragment of the POL/S genes containing 593 base pairs was amplified using nested PCR. Thirty four were PCR-positive and sequencing was performed using an ABI Prism 3130 Genetic Analyzer. Alignments and mutation mapping were performed using BioEdit software. Results HBV DNA from 21 blood donors and 13 untreated patient samples were characterized using nucleotide sequencing PCR products from the POL/S genes. We were able to detect one sample with the resistance mutation to lamivudine rtM204V?+?rtL180M (2.94%), which was found in a volunteer blood donor that has never used antiviral drugs. The other samples showed only compensatory mutations, such as rtL80F (5.88%), rtL80V (2.94%), rtL82V?+?rtV207L (2.94%), rtT128P (5.88%), rtT128N/S (2.94%) and rtS219A (5.88%). We found modifications in the S gene in 14 of the 34 samples (41.16%). The mutations detected were as follows: sM133L?+?sI195T (2.94%), sI195M (2.94%), sP120T (2.94%), sY100S/F (2.94%), sY100C (17.64%), sI/T126P?+?sQ129P (2.94%), sM198I?+?sF183C (2.94%) and sS210R (5.88%). Conclusions Our results suggest the transmission of lamivudine-resistant forms. Thus, the evaluation of HBV-infected subjects for lamivudine resistance would improve treatment regime. Moreover, the mutations in the S gene may impair HBsAg antigenicity and contribute to HBsAg failure detection and vaccine escape.



Fluorine substitution enhanced photovoltaic performance of a D-A(1)-D-A(2) copolymer.  


A new alternating donor-acceptor (D-A1-D-A2) copolymer containing two electron-deficient moieties, isoindigo and quinoxaline, was synthesized. The photovoltaic performance of this polymer could be improved by incorporating fluorine atoms into the quinoxaline units, resulting in an efficiency of 6.32%. This result highlights the attractive promise of D-A1-D-A2 copolymers for high-performance bulk heterojunction solar cells. PMID:24000353

Dang, Dongfeng; Chen, Weichao; Yang, Renqiang; Zhu, Weiguo; Mammo, Wendimagegn; Wang, Ergang




Microsoft Academic Search

RESUMO - A região cacaueira da Bahia vem enfrentando uma crise sem precedente em sua história com a redução continuada da renda e emprego em virtude de problemas enfrentados pela cultura do cacau. O crescimento do parque agroindustrial de polpa de frutas na região poderá contribuir na diversificação da economia regional ao criar uma opção de mercado para a produção

Antônio Carlos de Araújo; Lúcia Maria Ramos Silva; Ahmad Saeed Khan; José B. V. Leite; Aurélio F. Macedo


Obstáculos e potencialidades à competitividade das MPEs de calçados da Paraíba- um estudo da cooperação do aprendizado e da inovação  

Microsoft Academic Search

O objetivo deste trabalho é avaliar os obstáculos e potencialidades das micro e pequenas empresas da Paraíba, através do estudo da cooperação, aprendizado e inovação. Essas empresas estão concentradas espacialmente nos Arranjos Produtivos Locais (APLs) de Campina Grande e de Patos, onde há uma diversidade de atores públicos e privados, além de uma base institucional relevante. Para tanto, utilizou-se um

Janaina da Silva Alves



Microsoft Academic Search

O objetivo principal deste trabalho foi analisar o termo de consentimento para laqueadura de trompas perante a autonomia do corpo da mulher analisando-se os princípios e garantias constitucionais conseguidos a duras penas ao longo de muitos anos. Teve como método uma pesquisa bibliográfica envolvendo legislação e Doutrina, bem como contato direto com instituições e profissionais habilitados na área da saúde

Marcelo Nunes Apolinário; Carmen Liliam Rodrigues Arnoni




Microsoft Academic Search

RESUMO Neste trabalho, avaliou-se o efeito da posição da linha lateral de um equipamento de irrigação do tipo pivô central na uniformidade de distribuição de água, lâmina de irrigação aplicada, eficiência de aplicação, eficiência de armazenamento, adequabilidade da irrigação e velocidade de deslocamento da torre final. Foram avaliadas três posições: aclive d e 3 ,87%, nível e d eclive de

João Luis Zocoler; Luis Eduardo; Vilar Cesar; Luiz Sérgio Vanzela


Lyman Spectroscopy of Hot DA White Dwarfs  

NASA Astrophysics Data System (ADS)

Several hot (\\teff\\ > 20,000 K) DA white dwarfs were observed with the Hopkins Ultraviolet Telescope (HUT) during the recent ASTRO-2 mission. This is the first time that the full hydrogen Lyman series has been observed for a significant number of white dwarfs. The observations were undertaken as a critical test of Stark broadening theory and the Hummer-Mihalas occupation probability formalism (Hummer, D.G. and Mihalas, D. 1988, ApJ 331, 794); any departures were expected to be of much greater magnitude in the Lyman lines, given that they are much stronger than the Balmer lines. To span the widest possible range in \\teff\\ and \\logg the targets included Wolf 1346, GD 50, GD 71, GD 153, GD 394, RE0512-004, RE1738+669, HZ 43, and G191-B2B. These include one of the coolest DA without Lyman satellite features, Wolf 1346 (20,000 K), and the hottest known DA, RE1738+669 (95,000 K); one of the least massive DA, RE0512-004 (\\logg\\ = 7.3), and the most massive known DA, GD 50 (\\logg\\ = 9.2). (The temperatures and gravities listed are from pure H model fits to our optical spectra). The flight data are now being reduced, after which we will perform detailed comparisons of the Lyman and Balmer line profiles with models. We will be reporting on the initial results of that comparison.

Finley, David S.



Mulheres atletas: re-significações da corporalidade feminina  

Microsoft Academic Search

R R R R Resumo esumo esumo esumo esumo: A participação esportiva das mulheres contribui para uma re-significação da corporalidade feminina? Lembrando da idéia de Susan Brownmiller, para quem a feminilidade representa, na sociedade moderna, uma \\

Miriam Adelman



Geochemistry and origin of the early Mesoproterozoic mangerite-charnockite-rapakivi granite association of the Serra da Providência suite and associated gabbros, central-eastern Rondônia, SW Amazonian Craton, Brazil  

NASA Astrophysics Data System (ADS)

The Serra da Providência suite (SPS) is constituted of quartz mangerite-charnockite-rapakivi granite and coeval mafic rocks, intruded during the 1.60-1.53 Ga interval into Paleoproterozoic metamorphic basement (Jamari Complex) in the Juruena-Rondônia geochronologic province, SW Amazonian Craton. In this region the Serra da Providência suite consists of two batholiths and several small plutons represented by syeno-monzogranites and igneous quartz mangerite and charnockites. The Serra da Providência granites are ferroan, calc-alkalic to alkali-calcic and characterized by high Na2O + K2O, Rb, Zr, Y, Nb, Ta, Ce, Zn, Ga, and REE (except for Eu), moderate Ba, and low Sr, MgO, and CaO. Ga/Al, Y/Nb, FeOT/(FeOT + MgO) and K2O/Na2O ratios are high and Sr/Ba and Rb/Ba are low and are geochemically similar to typical A2-subtype granites. The SPS charnockites and quartz mangerites display values of FeOT, MgO, CaO, Ba, Rb, Sr, Nb/Ta and Zr/Hf similar to the SPS A-type granites. The occurrence of magnetite as an accessory phase and the high FeOT/(FeOT + MgO) in granites, charnockites and quartz mangerites suggest crystallization from a relatively oxidized magma and also explain the occurrence of titanite as a primary phase in these granites, similar to those reported in several localities of the Amazonian Craton. The parental magma of the mafic rocks of the SPS probably had mainly enriched sources and resulted from melting of heterogeneous mantle reservoirs with coherent chemical characteristics. Trace elements data indicate subduction-modified mantle sources for the mafic rocks that are in agreement with the post-collisional character of this magmatism. A large set of whole-rock Sm-Nd isotope data (Bettencourt et al., 1999; Scandolara, 2006; Santos et al., 2008 and this work) demonstrate that granites, charnockites and quartz mangerites of the study area are the product of magmas derived from interaction between enriched mantle derived magmas (in very subordinate proportions) and recycled crust in larger proportion. The source region is dominated by Paleoproterozoic material, but several samples yield Archaean model ages that are the first evidence for such ancient source materials in the SW Amazonian Craton. The Serra da Providência suite was emplaced during the post-collisional stage of the Juruena-Jamari arc and Madeirinha orogeny (Scandolara et al., 2011), and post-dates the collision between the Tapajós (Tapajós-Parima geochronological province, Pará, Brazil) and Bolívia (palaeocontinent which precedes Sunsás geochronological province) blocks. Its geochemical and structural features are coincident with those recognized in post-collisional granitoids. Geochemical data suggest that complex processes which involved crustal melting, fractional crystallization, magma mixing and, in some extent, crustal assimilation were responsible for the magmatism of the Serra da Providência suite. The magmas that constitute the Serra da Providência suite resulted from varying degrees of partial melting of a compositionally heterogeneous source. The nature of the source and the degree of melting exerted a significant control over compositional variation in some of the parental magmas which were also affected by subsequent fractional crystallization and mingling processes. Internal variations in composition observed in several bodies can be the result of incremental amalgamation of different magma pulses that varied mainly in the degree of partial melting.

Scandolara, Jaime E.; Fuck, Reinhardt A.; Dall'Agnol, Roberto; Dantas, Elton L.



Variable Importance in MANOVA and DA.  

ERIC Educational Resources Information Center

Some interpretations of relative variable importance in the contexts of multivariate analysis of variance (MANOVA) and discriminant analysis (DA) are presented. Some indices potentially useful for the interpretations are presented, and the assessment of variable importance is illustrated using real data sets. Both descriptive discriminant analysis…

Huberty, Carl J.; Wisenbaker, Joseph M.


IrDA: past, present and future  

Microsoft Academic Search

The core Internet technologies were in the hands of the research community 10 or more years before the World Wide Web happened and popularized the Internet as a place to find information, access service, and trade. The Infrared Data Association has been in existence for over six years. Products embedding the communication technology the IrDA defines have been around for

Stuart Williams



Excitation by gravity of DA and EDC waves  

NASA Astrophysics Data System (ADS)

Excitation of dust-acoustic (DA) and electrostatic dust-cyclotron (EDC) waves by gravity in a collisional plasma is considered. The fall of charged dust grains through the plasma may excite both DA and EDC waves. The case of an ionospheric plasma is considered, for which it is found that the EDC mode has a substantially larger growth than the DA mode.

D'Angelo, N.




Microsoft Academic Search

Ciro dos Anjos Abdias and A menina do sobrado O presente trabalho apresenta um breve estudo sobre os direitos fundamentais da mulher, fazendo um retrocesso de sua posição na sociedade, desde os tempos bíblicos, quando surgiu o mito de que fora responsável pela queda do homem, iniciando-se sua discriminação. Foi feito um levantamento da situação da mulher em diversos países,



SWEETCat I. Stellar parameters for host stars (Santos+, 2013)  

NASA Astrophysics Data System (ADS)

The file sweetcat.dat contains the spectroscopic parameters for all the planet hosts compiled for this work (data as of July 2013). Up to date tables can be found at . The spectra were gathered through observations, made by our team, and by the use of the ESO archive. In total, six different spectrographs were used: FEROS (2.2m ESO/MPI telescope, La Silla, Chile), FIES (Nordic Optical Telescope, La Palma, Spain), HARPS (3.6m ESO telescope, La Silla, Chile), SARG (TNG Telescope, La Palma, Spain), SOPHIE (1.93m telescope, OHP, France), and UVES (VLT Kueyen telescope, Paranal, Chile). (2 data files).

Santos, N. C.; Sousa, S. G.; Mortier, A.; Neves, V.; Adibekyan, V.; Tsantaki, M.; Delgado Mena, E.; Bonfils, X.; Israelian, G.; Mayor, M.; Udry; S.



Infant feeding practices in a poor district of Santo Domingo.  


During August-December 1989, in the Dominican Republic, local health promoters interviewed 103 primary caretakers and took anthropometric measurements from 103 children aged 3 years and under during home visits in three poor neighborhoods of Los Alcarrizos (Alto de Chavon, Barrio Landia, and Pueblo Nuevo) to examine infant feeding practices. 95% of the mothers had started breast feeding. The median duration of breast feeding was 7.5 months. Almost 33% had quit breast feeding by 3 months. Perception of insufficient milk was the leading reason for early discontinuation of exclusive breast feeding. The local pharmacies in all three communities offered powdered milk. About 25% of mothers began using breast milk substitutes within the first week. A non-infant formula milk powder was the most commonly used breast milk substitute. 30% of mothers stored prepared milk for later feeds, as long as half a day. Only 13% of households had a refrigerator. The most frequently used first weaning foods were orange juice, lime juice, and beans. 76% of mothers had used or were currently using baby bottles. 95% of them reported washing the bottle in boiling water. The median age of introducing the baby bottle was 3 days. Only 44% of mother covered prepared food during storage. The most commonly eaten foods among children aged at least 1 year were milk, beans, rice, and citrus fruits. Yet the children did not eat all these foods daily. 18% of 1-2 year olds and 13% of 2-3 year olds did not eat meats and alternatives regularly. 7% of 1-2 year olds and 10% of 2-3 year olds did not eat fruits and vegetables regularly. 4% of 1-2 year olds and 3% of 2-3 year olds did not eat breads and cereals regularly. About 14% of all children were not consuming any milk at the time of the survey. These findings will be used to refine nutrition education programs in the district to make them more effective. PMID:12290747

Mclennan, J D; Spady, D W




Microsoft Academic Search

Resumo: Procura o presente estudo traçar um breve panorama da situação da mulher no que concerne à proteção da sua dignidade. Em primeiro lugar, faz-se um breve histórico sobre o estabelecimento de convenções e outros instrumentos internacionais de proteção à dignidade da mulher. Em segundo lugar, apontam-se os grandes desafios enfrentados na atualidade para a efetiva proteção dos direitos da

Juliana Oliveira Domingues; Santa Marcelina


Immunochemical and immunohistochemical studies, using antisera against porcine 25 kDa amelogenin, 89 kDa enamelin and the 13–17 kDa nonamelogenins, on immature enamel of the pig and rat  

Microsoft Academic Search

Enamel proteins were extracted from the newly formed layer of immature porcine enamel, and the 25 kDa amelogenin, 89 kDa enamelin and 13–17 kDa nonamelogenins were purified. Specific antisera were raised against these proteins. Antibodies specific to the C-terminal region (residues 149–173) of the 25 kDa amelogenin were generated by absorption of the anti-25 kDa amelogenin serum with 20 kDa

T. Uchida; T. Tanabe; M. Fukae; M. Shimizu; Mo Yamada; K. Miake; S. Kobayashi



The Finuda Experiment at DA?NE  

NASA Astrophysics Data System (ADS)

The FINUDA (FIsica NUcleare a DA?NE) experiment was built in order to study the interactions of stopped K- on nuclei, with emphasis on Hypernuclear spectroscopy and Hypernuclei decays. Due to the nice performances of the spectrometer, it proved also suited to study, more generally, final states with several particles and able to recostruct secondary vertices, hence greatly enlarging the physical topics that could be addressed.

Lucherini, V.



The Finuda Experiment at DA?NE  

NASA Astrophysics Data System (ADS)

The FINUDA (FIsica NUcleare a DA?NE) experiment was built in order to study the interactions of stopped K- on nuclei, with emphasis on Hypernuclear spectroscopy and Hypernuclei decays. Due to the nice performances of the spectrometer, it proved also suited to study, more generally, final states with several particles and able to recostruct secondary vertices, hence greatly enlarging the physical topics that could be addressed.

Lucherini, V.


The Finuda Experiment on DA?NE  

NASA Astrophysics Data System (ADS)

FINUDA (FIsica NUcleare a DA?ne) is a spectrometer built to study the interactions of stopped K- on nuclei, with emphasis on spectroscopy and decay of ?-hypernuclei. Due to the nice performances of the apparatus and its ability to reconstruct secondary vertices, it proved suited to study also final states with several particles (including neutron), hence greatly enlarging the physical topics that could be addressed.

Lucherini, Vincenzo


Raízes pós-mitológicas da Astronomia Clássica  

NASA Astrophysics Data System (ADS)

Apresentamos como a invenção de um modelo astronômico e cosmológico fundado em causas naturais foi um processo gestado num ambiente cultural específico, o do advento do pensamento pós-mitológico, que tem como sua raiz mais funda a trajetória particular da Grécia Antiga, com ênfase na resolução de uma grave crise social e a evolução do mundo clássico para o contexto espiritual de um império universal.

di Bartolo, L.; Villas da Rocha, J. F.



Leonardo da Vinci's studies of the heart.  


Leonardo da Vinci's detailed drawings are justly celebrated; however, less well known are his accounts of the structures and functions of the organs. In this paper, we focus on his illustrations of the heart, his conjectures about heart and blood vessel function, his experiments on model systems to test those conjectures, and his unprecedented conclusions about the way in which the cardiovascular system operates. In particular, da Vinci seems to have been the first to recognize that the heart is a muscle and that systole is the active phase of the pump. He also seems to have understood the functions of the auricles and pulmonary veins, identified the relationship between the cardiac cycle and the pulse, and explained the hemodynamic mechanism of valve opening and closure. He also described anatomical variations and changes in structure and function that occurred with age. We outline da Vinci's varied career and suggest ways in which his personality, experience, skills and intellectual heritage contributed to these advances in understanding. We also consider his influence on later studies in anatomy and physiology. PMID:23044431

Shoja, Mohammadali M; Agutter, Paul S; Loukas, Marios; Benninger, Brion; Shokouhi, Ghaffar; Namdar, Husain; Ghabili, Kamyar; Khalili, Majid; Tubbs, R Shane



A computational theory of da Vinci stereopsis.  


In binocular vision, occlusion of one object by another gives rise to monocular occlusions—regions visible only in one eye. Although binocular disparities cannot be computed for these regions, monocular occlusions can be precisely localized in depth and can induce the perception of illusory occluding surfaces. The phenomenon of depth perception from monocular occlusions, known as da Vinci stereopsis, is intriguing, but its mechanisms are not well understood. We first propose a theory of the mechanisms underlying da Vinci stereopsis that is based on the psychophysical and computational literature on monocular occlusions. It postulates, among other principles, that monocular areas are detected explicitly, and depth from occlusions is calculated based on constraints imposed by occlusion geometry. Next, we describe a biologically inspired computational model based on this theory that successfully reconstructs depth in a large range of stimuli and produces results similar to those described in the psychophysical literature. These results demonstrate that the proposed neural architecture could underpin da Vinci stereopsis and other stereoscopic percepts. PMID:24914063

Tsirlin, Inna; Wilcox, Laurie M; Allison, Robert S



A Influência da Escola e da Mídia na Obtenção dos Conhecimentos Astronômicos  

NASA Astrophysics Data System (ADS)

O universo em que vivemos possui segredos jamais imaginados pelo homem, fruto da pequena proporção que ocupamos diante de um cosmos infinito. A astronomia funciona como um instrumento que sacia a sede humana em desvendar esses segredos, estudando as galáxias, quasares, pulsares além da existência de todos os elementos que preenchem o espaço cósmico, o vácuo, não mais vazio como se pensou em outros tempos. A mídia explora esse campo de forma mística e também científica. A difusão adequada e dentro dos padrões educacionais vigentes, de conceitos astronômicos, se faz necessária, devido sua presença nos conteúdos escolares, centros de ciência e na mídia, de modo geral. A reforma do Currículo Básico da Escola Pública da maioria dos Estados brasileiros introduziu conceitos astronômicos desde a pré-escola até o Ensino Médio. Num estudo realizado com 1.032 alunos do Ensino Médio de seis escolas estaduais, revelou-se através da aplicação de um questionário que abordava conheciment os básicos em astronomia, como estes foram adquiridos e qual era a infra-estrutura oferecida pela escola: para 66,2% dos entrevistados o professor já fez alguma apresentação a respeito de astronomia. O presente estudo revelou também que 44,5% dos alunos entrevistados adquiriram seus conhecimentos astronômicos na escola, 28,1% através da televisão e 27,4% afirmaram que seus conhecimentos foram obtidos através de outros tipos de mídia (internet, filmes e revistas). Embora o estudo revele claramente que há certo equilíbrio na forma em que os alunos obtiveram seus conhecimentos astronômicos, a mídia ainda explora e muitas vezes desperta o interesse pela astronomia com assuntos que não fazem parte de nosso cotidiano como, por exemplo, o homem na Lua, os projetos espaciais e a primeira viagem de um astronauta brasileiro no espaço. Porém, em certos casos, a mídia pode promover conhecimentos incompletos ou mesmo equivocados sobre os conceitos que envolvam a astronomia, como por exemplo a propagação do som no vácuo observado na maioria dos filmes de f icção científica ou da existência das estações do ano em função da distância da Terra ao Sol, em conformidade com o questionário aplicado.

da Cunha, W. S.; Voelzke, M. R.; Amaral, L. H.



Análise da Evolução e da Estrutura da Siderurgia Brasileira e do Uso de Carvão Vegetal no período de 1980 a 2006  

Microsoft Academic Search

O presente trabalho analisa a evolução e estrutura da siderurgia brasileira e do uso do carvão vegetal no período de 1980 a 2006. Os objetivos específicos são: 1) analisar a evolução da produção, consumo, exportação e importação dos principais produtos elaborados por esta indústria, separando os que usam carvão vegetal e os que usam carvão mineral; e 2) caracterizar a

Thais Hortense De Carvalho; Carlos Jose Caetano Bacha



The ATLAS PanDA Monitoring System and its Evolution  

Microsoft Academic Search

The PanDA (Production and Distributed Analysis) Workload Management System is used for ATLAS distributed production and analysis worldwide. The needs of ATLAS global computing imposed challenging requirements on the design of PanDA in areas such as scalability, robustness, automation, diagnostics, and usability for both production shifters and analysis users. Through a system-wide job database, the PanDA monitor provides a comprehensive

A Klimentov; P Nevski; M Potekhin; T Wenaus



Photospheric metals in hot DA white dwarfs  

NASA Astrophysics Data System (ADS)

Previous observations of the soft X-ray and EUV region with the Einstein, EXOSAT, and ROSAT satellites have indicated the presence of photospheric absorbers in most DA white dwarfs with {T_eff} ga 40000K. Several of these objects have now been observed with the Extreme Ultraviolet Explorer (EUVE). Since the detection of individual metal lines is rather difficult with EUVE spectra we chose the well studied DA G191-B2B as reference object for the analysis of other white dwarfs. In spectra obtained with the GHRS of the Hubble Space Telescope photospheric lines of nitrogen, silicon, iron, and nickel could be detected. With the abundances determined from these observations we were able to reproduce the EUVE spectrum of G191-B2B. The main source of EUV opacity turned out to be iron, followed by nickel. The analysis of both the EUV continuum and the hydrogen Balmer lines led to {T_eff} = 56000 +/- 2000K. For the analysis of other DA white dwarfs we used the same relative abundances as for G191-B2B but introduced a free scaling factor (``metallicity'') for the total amount of metals. From EUVE spectra we determined relative metallicities for 20 objects with {T_eff} ga 40000K. The main result is that all DAs with {T_eff} ga 50000K contain additional photospheric absorbers, whereas at lower temperatures all observations, with the exception of GD394, are compatible with pure hydrogen atmospheres. As in the case of G191-B2B, iron determines also the EUV opacity in the objects at {T_eff} ga 50000K. This is in agreement with radiative levitation theory which predicts the support of iron above this temperature. Based on observations with the HST and EUVE satellites, and on optical spectra obtained at the DSAZ Calar Alto

Wolff, B.; Koester, D.; Dreizler, S.; Haas, S.




Microsoft Academic Search

1. Introdução Este estudo tem por objetivo fazer uma reflexão, sob a luz da análise do discurso da linha francesa, acerca do humor na literatura, mais precisamente, da linguagem transgressiva que se instaura no discurso literário-teatral em decorrência de fenômenos como ironia e paródia. Esse enfoque será norteado a partir da peça Calabar - o elogio da traição (1973), de

Manuella Felicíssimo


Levantamento dos Fatores Limitantes da Produtividade Leiteira na Regi- ão de Anápolis-Go  

Microsoft Academic Search

O presente trabalho foi realizado no município de Anápolis-GO no mês de julho de 1995. Consistiu de uma avaliação da atividade pecuária em fazendas leiteiras com o objetivo de, através da análise dos principais índices zootécnicos, determinar quais os fatores limitantes para o aumento da produtividade. Constou da aplicação de um questionário de levantamento sócio-econômico da produção em uma amostra



40 CFR 60.49Da - Emission monitoring.  

Code of Federal Regulations, 2011 CFR

...60.44Da(d)(1), and 60.44Da(e). (1) The owner or operator of an affected facility with electricity generation shall install, calibrate, maintain, and operate a wattmeter; measure gross electrical output in MWh on...



Research on Wireless OPC DA Server for Modbus  

Microsoft Academic Search

Based on the study of the drawback of traditional wired automatic control system and current wireless remote monitoring system for Modbus, and the utilization of a Uniform Wireless OPC DA Server, a new solution to construct a wireless OPC DA server for Modbus was given out. It used mobile communication networks and OPC technology. Also, Modbus request command strategy was

Xiaoping Zhou; Xiaoxuan Ma; Hantao Zhang



The Case: Bunche-Da Vinci Learning Partnership Academy  

ERIC Educational Resources Information Center

The Bunche-Da Vinci case described in this article presents a situation at Bunche Elementary School that four theorists were asked to address in their evaluation designs (see EJ791771, EJ719772, EJ791773, and EJ792694). The Bunche-Da Vinci Learning Partnership Academy, an elementary school located between an urban port city and a historically…

Eisenberg, Nicole; Winters, Lynn; Alkin, Marvin C.



Asteroid 2012 DA14 To Whiz Past Earth Safely  

NASA Video Gallery

The small near-Earth asteroid 2012 DA14 will pass very close to Earth on Feb. 15, 2013. Asteroid 2012 DA14 will be closest to Earth at about 11:24 a.m. PST (2:24 p.m. EST and 1924 UTC), on Feb. 15,...


DA-6034 Induces [Ca2+]i Increase in Epithelial Cells  

PubMed Central

DA-6034, a eupatilin derivative of flavonoid, has shown potent effects on the protection of gastric mucosa and induced the increases in fluid and glycoprotein secretion in human and rat corneal and conjunctival cells, suggesting that it might be considered as a drug for the treatment of dry eye. However, whether DA-6034 induces Ca2+ signaling and its underlying mechanism in epithelial cells are not known. In the present study, we investigated the mechanism for actions of DA-6034 in Ca2+ signaling pathways of the epithelial cells (conjunctival and corneal cells) from human donor eyes and mouse salivary gland epithelial cells. DA-6034 activated Ca2+-activated Cl- channels (CaCCs) and increased intracellular calcium concentrations ([Ca2+]i) in primary cultured human conjunctival cells. DA-6034 also increased [Ca2+]i in mouse salivary gland cells and human corneal epithelial cells. [Ca2+]i increase of DA-6034 was dependent on the Ca2+ entry from extracellular and Ca2+ release from internal Ca2+ stores. Interestingly, these effects of DA-6034 were related to ryanodine receptors (RyRs) but not phospholipase C/inositol 1,4,5-triphosphate (IP3) pathway and lysosomal Ca2+ stores. These results suggest that DA-6034 induces Ca2+ signaling via extracellular Ca2+ entry and RyRs-sensitive Ca2+ release from internal Ca2+ stores in epithelial cells.

Yang, Yu-Mi; Park, Soonhong; Ji, HyeWon; Kim, Tae-im; Kim, Eung Kweon; Kang, Kyung Koo



Berengario da Carpi: a pioneer in neurotraumatology.  


Berengario da Carpi was one of the most famous physicians of the 16th century, a recognized master of anatomy and surgery, an emblematic "Renaissance man" who combined his medical experience and engineering knowledge to design new surgical instruments, and effectively used the arts of writing and drawing to describe state-of-the-art medicine and provide illustrations of anatomical structures. His greatest contribution to medicine was to write the most important work on craniocerebral surgery of the 16th century, the Tractatus de Fractura Calvae sive Cranei (Treatise on Fractures of the Calvaria or Cranium), in which he described an entire set of surgical instruments to be used for cranial operations to treat head traumas that became a reference for later generations of physicians. This was a systematic treatise covering the mechanisms, classification, and medical and surgical treatment of head traumas, and can be considered a milestone in the history of neurotraumatology. PMID:21128734

Di Ieva, Antonio; Gaetani, Paolo; Matula, Christian; Sherif, Camillo; Skopec, Manfred; Tschabitscher, Manfred



Estrés y dolor crónico: una perspectiva endocrinológica  

Microsoft Academic Search

Chronic stress, understood as a disturbance of the body homeostasis, is partially driven by many hormonal pathways. Prolactine, TSH (Thyrotropin), vasopresin, FSH (Follicle-Stimulating Hormone), LH (Luteinizing Hormone), and GH (Growth Hormone) have been involved in many stress reactions. In acute stress, there are many evidences for the increased both cathecolaminergic and hypothalamic-pituitary-adrenal axis. In chronic conditions, these hyperactivations are controversial

Guillem Cuatrecasas Cambra



Sobre a largura da última superfície de espalhamento  

NASA Astrophysics Data System (ADS)

De acordo com o modelo do "Big-Bang", no universo primordial a matéria estava em equilíbrio térmico com a radiação. Com a expansão a temperatura da radiação cai. Quando a temperatura chega em torno dos 4.000K, os espalhamentos diminuem, começando a recombinação dos prótons e elétrons em Hidrogênio neutro (era conhecida como da recombinação). Ao final da recombinação, os fótons se propagam livremente sofrendo, em princípio, somente os efeitos do "redshift" cosmológico. Esses fótons nos alcançam hoje como a radiação cósmica de fundo (RCF), e parecem vir de uma superfície esférica ao nosso redor, tal que o raio dela é a distância que ele viajou desde seu último espalhamento na época da recombinação. Naturalmente, esse processo não ocorreu abruptamente, implicando na existência de uma largura no espaço dos "redshifts" que deve depender do modelo cosmológico específico e dos processos físicos considerados. Neste trabalho analisamos os efeitos de diferentes modelos - a saber, aqueles com decaimento do vácuo L(t), criação de matéria, quintessência e gás de Chaplygin - sobre a última superfície de espalhamento da RCF, em particular sua largura e a função visibilidade, que determina a probabilidade de um fóton ter tido seu último espalhamento num "redshift" z. No caso particular dos modelos com decaimento do vácuo, existe uma forte dependência da função visibilidade com L(t). Tais efeitos poderão ser testados através da análise dos resultados de experimentos mais precisos que estão atualmente em andamento, como por exemplo, o WMAP.

Nobre, M. A. S.; Pires, N.; Lima, J. A. S.



HeDaM: The Herschel Database in Marseille  

NASA Astrophysics Data System (ADS)

The Herschel Database in Marseille (HeDaM) is an information system operated by the Laboratoire d'Astrophysique de Marseille (LAM) datacenter — CeSAM. Gathering data from various Herschel Space Observatory surveys, HeDaM serves both as a collaboration tool between scientists and as a way to distribute public data to a wider community. With HeDaM, users have access to the direct download of catalogs and maps, but also to value-adding services such as: multi-? FITS cut-outs, integration with Aladin applet and Topcat, and list-based cone searches.

Roehlly, Y.; Buat, V.; Heinis, S.; Moreau, C.; Gimenez, S.



Metal Lines in DA White Dwarfs  

NASA Astrophysics Data System (ADS)

We report Keck telescope HIRES echelle observations of DA white dwarfs in a continuation of an extensive search for metals. These spectra are supplemented with new JHK magnitudes that are used to determine improved atmospheric parameters. Of the DA white dwarfs not in binary or common proper motion systems, about 25% show Ca II lines. For these, Ca abundances are determined from comparison with theoretical equivalent widths from model atmosphere calculations; in a few cases we also obtain Mg, Fe, Si, and Al abundances. If Ca is not observed, we generally determine very stringent upper limits. We compare the data to predictions of previously published models involving the accretion/diffusion of interstellar matter and of comets. The derived abundances are not obviously compatible with the predictions of either model, which up to now could only be tested with traces of metals in helium-rich white dwarfs. By modifying certain assumptions in the published interstellar accretion model we are able to match the distribution of the elements in the white dwarf atmospheres, but, even so, tests of other expectations from this scenario are less successful. Because comet accretion appears unlikely to be the primary cause of the DAZ phenomenon, the data suggest that no more than about 20% of F-type main-sequence stars are accompanied by Oort-like comet clouds. This represents the first observational estimate of this fraction. A plausible alternative to the accretion of cometary or interstellar matter is disruption and accretion of asteroidal material, a model first suggested in 1990 to explain excess near-infrared emission from the DAZ G29-38. An asteroidal debris model to account for the general DAZ phenomenon does not presently disagree with the HIRES data, but neither is there any compelling evidence in support of such a model. The HIRES data indicate that in close red dwarf/white dwarf binaries not known to be cataclysmic variables there is, nonetheless, significant mass transfer, perhaps in the form of a wind flowing off the red dwarf. As a by-product we find from the kinematics of GD 165 a likely age of more than 2 Gyr for its probable brown dwarf companion GD 165B. This paper is based in part on observations obtained at the Calar Alto Observatory of the Deutsch-Spanisches Astronomisches Zentrum and at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and NASA. The Observatory was made possible by the generous financial suppport of the W. M. Keck Foundation. We have made use of the SIMBAD database at CDS.

Zuckerman, B.; Koester, D.; Reid, I. N.; Hünsch, M.



Circumstellar features in hot DA white dwarfs  

NASA Astrophysics Data System (ADS)

We present a phenomenological study of highly ionized, non-photospheric absorption features in high spectral resolution vacuum ultraviolet spectra of 23 hot DA white dwarfs. Prior to this study, four of the survey objects (Feige 24, REJ 0457-281, G191-B2B and REJ 1614-085) were known to possess these features. We find four new objects with multiple components in one or more of the principal resonance lines: REJ 1738+665, Ton 021, REJ 0558-373 and WD 2218+706. A fifth object, REJ 2156-546, also shows some evidence of multiple components, though further observations are required to confirm the detection. We discuss possible origins for these features including ionization of the local interstellar environment, the presence of material inside the gravitational well of the white dwarf, mass loss in a stellar wind and the existence of material in an ancient planetary nebula around the star. We propose ionization of the local interstellar medium as the origin of these features in G191-B2B and REJ 1738+665, and demonstrate the need for higher-resolution spectroscopy of the sample, to detect multiple interstellar medium velocity components and to identify circumstellar features that may lie close to the photospheric velocity.

Bannister, N. P.; Barstow, M. A.; Holberg, J. B.; Bruhweiler, F. C.




Microsoft Academic Search

RESUMO A presença cada vez mais marcante das tecnologias de comunicação e informação no cotidiano dos sujeitos tem alertado aos profissionais da educação para a necessidade de construir e utilizar a potencialidade dessas ferramentas. O objetivo deste artigo é mostrar que os pressupostos da Teoria da Complexidade, num ambiente virtual, contribuem de forma decisiva para potencializar a aprendizagem da língua

Dulci Marlise BOETTCHER


Tree Branching: Leonardo da Vinci's Rule versus Biomechanical Models  

PubMed Central

This study examined Leonardo da Vinci's rule (i.e., the sum of the cross-sectional area of all tree branches above a branching point at any height is equal to the cross-sectional area of the trunk or the branch immediately below the branching point) using simulations based on two biomechanical models: the uniform stress and elastic similarity models. Model calculations of the daughter/mother ratio (i.e., the ratio of the total cross-sectional area of the daughter branches to the cross-sectional area of the mother branch at the branching point) showed that both biomechanical models agreed with da Vinci's rule when the branching angles of daughter branches and the weights of lateral daughter branches were small; however, the models deviated from da Vinci's rule as the weights and/or the branching angles of lateral daughter branches increased. The calculated values of the two models were largely similar but differed in some ways. Field measurements of Fagus crenata and Abies homolepis also fit this trend, wherein models deviated from da Vinci's rule with increasing relative weights of lateral daughter branches. However, this deviation was small for a branching pattern in nature, where empirical measurements were taken under realistic measurement conditions; thus, da Vinci's rule did not critically contradict the biomechanical models in the case of real branching patterns, though the model calculations described the contradiction between da Vinci's rule and the biomechanical models. The field data for Fagus crenata fit the uniform stress model best, indicating that stress uniformity is the key constraint of branch morphology in Fagus crenata rather than elastic similarity or da Vinci's rule. On the other hand, mechanical constraints are not necessarily significant in the morphology of Abies homolepis branches, depending on the number of daughter branches. Rather, these branches were often in agreement with da Vinci's rule.

Minamino, Ryoko; Tateno, Masaki




Microsoft Academic Search

In the present article we review engineering and research efforts conducted by a group of volunteers with the help of advanced engineering commercial software (CFD-ACE+, ANSYS, CFD-FASTRAN, Matlab\\/Simulink, Autodesk Inventor, Maple) in support of the da Vinci Project, the first Canadian competitor in the International X Prize Competition. The da Vinci Project has an objective of launching the first commercial

Vladimir Kudriavtsev; Brian Feeney; Max Buneta; James Porcher; Asier Ania; Michael Trauttmansdorff; Ta-Liang Hsu; Marek Krzeminski; Kalman Rooz


Variação da qualidade da madeira entre grupos fenotípicos de clones de Eucalyptus urophylla S.T. Blake da Ilha de Flores, Indonesia Wood quality variation among phenotypic groups of clones Eucalyptus urophylla S.T. Blake from Flores Island, Indonesia  

Microsoft Academic Search

RESUMO: O objetivo do presente trabalho foi estudar parâmetros de qualidade da madeira (densidade básica, comprimento, largura, diâmetro e espessura da parede das fibras) de clones de Eucalyptus urophylla, da Ilha de Flores, Indonésia, agrupados pelas características silviculturais e botânicas. A população base constituiu-se de um banco clonal, com 6 anos de idade, implantado no Horto Florestal Nossa Senhora Aparecida,

Osmar Francisco Ruy; Mário Ferreira; Mário Tomazello Filho


Estudo de não gaussianidade nas anisotropias da RCF medidas Wmap  

NASA Astrophysics Data System (ADS)

A investigação do campo de flutuações da Radiação Cósmica de Fundo (RCF) pode oferecer um importante teste para os modelos cosmológicos que descrevem a origem e a evolução das flutuações primordiais. De um lado, apresenta-se o modelo inflacionário que prevê um espectro de flutuações adiabáticas distribuídas segundo uma gaussiana e, de outro, os modelos de defeitos topológicos (dentre outros) que descrevem um mecanismo para a geração de flutuações de isocurvatura que obedecem a uma distribuição não gaussiana. Este trabalho tem como objetivo caracterizar traços do modelo não gaussiano de campo misto (entre flutuações adiabáticas e de isocurvatura) nos mapas do Wilkinson Microwave Anisotropy Probe (WMAP). Simulações das anisotropias da RCF no contexto de mistura indicam traços marcantes na distribuição das flutuações de temperatura, mesmo quando consideradas pequenas contribuições do campo de isocurvatura (da ordem de 0.001). O efeito da mistura entre os campos resulta na transferência de potência de flutuações em escalas angulares intermediárias para flutuações em pequenas escalas angulares. Este efeito pode ser caracterizado pela relação entre as amplitudes dos primeiros picos acústicos no espectro de potência da RCF. Neste trabalho, investigamos a contribuição do campo de isocurvatura, no contexto de mistura, sobre as observações recentes da RCF realizadas pelo WMAP. As previsões do modelo de campo misto, uma vez confrontadas com as observações em pequenas escalas angulares, podem ajudar a revelar a natureza das flutuações primordiais.

Andrade, A. P. A.; Wuensche, C. A.; Ribeiro, A. L. B.



Hasty?yurv?da--a complete treatise on elephants.  


Ayurv?da, the oldest existing medical science of India, which is not only responsible for the health of human beings but also, plays an important in Veterinary sciences. In India, history of traditional Veterinary medicine dates back to the era of Mah?bh?rata i.e.5000 B.C., recorded in the form of "Nakula Samhhit?". Hast?yurv?da is a treatise on elephants, Palk?pya wrote this Samhit?. The present book is available as a complete Samhit?, edited by Pandit Shivadutta Sharma. Hast?yurv?da was printed at Anand??rama press in 1894. Hast?yurv?da has 160 Adhy?y?s. This Samhit? is based on fundamental principles of Ayurv?da, containing all aspects of Sharir including Anatomy & Physiology, Rog Vigy?na, Surgery and Treatment of elephants as well as their care. It is the one and only complete printed Samhit? and rare book on Hast?yurv?da by Palk?pya. PMID:18175649

Sharma, Mahesh Chandra



Autoras de obras didáticas e livros para o ensino da leitura produzidos no Rio Grande do Sul: contribuições à história da alfabetização (1950-1970)1  

Microsoft Academic Search

Resumo: A História da Alfabetização tem se constituído um importante campo de pesquisa no Brasil nos últimos anos, enfatizando, entre outras coisas, métodos e processos de ensino da leitura e da escrita, materiais de alfabetização, com destaque para as cartilhas escolares, histórias e trajetórias de vida de professoras alfabetizadoras, etc. Na pesquisa que realizamos desde 2001, temos privilegiado a análise

Eliane Peres


Modelagem do vento e da fotosfera de AG Carinae  

NASA Astrophysics Data System (ADS)

A trajetória evolutiva das estrelas de alta massa depende fortemente de suas taxas de perda de massa. Apesar do rápido progresso no estudo destas estrelas, a taxa de perda de massa e outros parâmetros físicos básicos, como a temperatura superficial e a velocidade terminal do vento ainda não estão bem determinados. Isto ocorre devido à presença de ventos irregulares, rápidos e fortes ao redor destas estrelas, tornando a interpretação dos seus espectros uma tarefa difícil. Assim, a modelagem do vento e da fotosfera dessas estrelas está sendo cada vez mais usada para obter tais parâmetros a partir dos espectros. O aumento da taxa de perda de massa durante a fase LBV (Variáveis Luminosas Azuis), comparado com outros tipos de estrelas, tem sido atribuído a instabilidades do tipo S Doradus. Dispomos de uma base de dados espectroscópicos cobrindo 22 anos de observações de AG Carinae, incluindo um ciclo S Doradus completo, com espectros CCD em alta resolução na faixa óptica e infravermelha. Utilizamos o programa desenvolvido por Schmutz (1997) para uma análise preliminar desse ciclo, obtendo a taxa de perda de massa a partir da linha do Ha. Não existe uma correlação clara da taxa de perda de massa com mudanças da temperatura efetiva, do raio da estrela e do fluxo na banda V. A estrela atingiu seu mínimo fotométrico (raio mínimo) em 1990 e o máximo fotométrico (raio máximo) em 1995, enquanto que o fluxo máximo da linha do Ha ocorreu em 1996. Além disso a taxa de perda de massa não segue esse ciclo, contrariamente às idéias correntes. Para fazer um modelo mais realista estamos usando o programa CMFGEN (Hillier & Miller), que trata a fotosfera e o vento estelar de forma consistente, considerando a radiação fora do equilíbrio termodinâmico (NLTE) e com blanketting total de linhas. Simulamos o espectro de AG Carinae em duas épocas extremas do ciclo S Dor para testar os resultados obtidos com o modelo mais simplificado.

Groh, J. H.; Damineli, A.



The Composition and Evolution of Extremely Hot DA White Dwarfs  

NASA Astrophysics Data System (ADS)

We propose high S/N {15:1 to 30:1} STIS far UV observations of the hottest known DA white dwarfs. With temperatures in the range 70, 000-130, 000K, these stars represent an important but little understood link between the H-rich central stars of planetary nebulae {CPN} and the general population of DA white dwarfs. Stellar temperatures are extremely difficult to determine in this range and depend upon knowledge of the photospheric composition, which is also a mystery. Consequently, their relationship to the cooler DA stars remains undefined. Far UV measurements of the abundances of heavy elements are essential for an ultimate determination of the stellar temperature. Coupling STIS data with an analysis of the Lyman lines obtained with planned FUSE observations, it will be possible to determine the physical parameters of these objects and finally track their evolution along the DA cooling sequence. In addition, we will search for evidence of mass outflow, in the form of blueward asymmetries in the heavy element line profiles, which is believed to cause departures from the compositions predicted by radiative levitation theory but which has been observed in only two DA white dwarfs.

Barstow, Martin



The ATLAS PanDA Monitoring System and its Evolution  

NASA Astrophysics Data System (ADS)

The PanDA (Production and Distributed Analysis) Workload Management System is used for ATLAS distributed production and analysis worldwide. The needs of ATLAS global computing imposed challenging requirements on the design of PanDA in areas such as scalability, robustness, automation, diagnostics, and usability for both production shifters and analysis users. Through a system-wide job database, the PanDA monitor provides a comprehensive and coherent view of the system and job execution, from high level summaries to detailed drill-down job diagnostics. It is (like the rest of PanDA) an Apache-based Python application backed by Oracle. The presentation layer is HTML code generated on the fly in the Python application which is also responsible for managing database queries. However, this approach is lacking in user interface flexibility, simplicity of communication with external systems, and ease of maintenance. A decision was therefore made to migrate the PanDA monitor server to Django Web Application Framework and apply JSON/AJAX technology in the browser front end. This allows us to greatly reduce the amount of application code, separate data preparation from presentation, leverage open source for tools such as authentication and authorization mechanisms, and provide a richer and more dynamic user experience. We describe our approach, design and initial experience with the migration process.

Klimentov, A.; Nevski, P.; Potekhin, M.; Wenaus, T.



1991 DA: An asteroid in a bizarre orbit  

NASA Technical Reports Server (NTRS)

Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that 1991 DA is an extinct or dormant comet. Previous CCD imaging has shown no indication of a coma; spectroscopic observations of 1991 DA which lack any evidence of strong comet-like emissions are reported. Numerical integrations of the orbit of this object were performed which show that is has been remarkably stable for the past approximately 20,000 yr, but chaotic before that. This may allow a new estimate to be made of the physical lifetimes of comets.

Steel, Duncan; Mcnaught, Robert H.; Asher, David



Geomagnetic observations on tristan da cunha, south atlantic ocean  

USGS Publications Warehouse

Few geomagnetic ground observations exist of the Earth's strongest core field anomaly, the South Atlantic Anomaly (SAA). The geomagnetic repeat station on the island Tristan da Cunha, located half-way between South Africa and South America at 37?? 05' S, 12?? 18' W, is therefore of crucial importance. We have conducted several sets of repeat station measurements during magnetically quiet conditions (Kp 2o or less) in 2004. The procedures are described and the results are compared to those from earlier campaigns and to the predictions of various global field models. Features of the local crustal bias field and the solar quiet daily variation are discussed. We also evaluate the benefit of continuous magnetic field recordings from Tristan da Cunha, and argue that such a data set is a very valuable addition to geomagnetic satellite data. Recently, funds were set up to establish and operate a magnetometer station on Tristan da Cunha during the Swarm magnetic satellite mission (2011-2014).

Matzka, J.; Olsen, N.; Maule, C. F.; Pedersen, L. W.; Berarducci, A. M.; Macmillan, S.



The Potential da Vinci in All of Us  

NSDL National Science Digital Library

The study of the human form is fundamental to both science and art curricula. For vertebrates, perhaps no feature is more important than the skeleton to determine observable form and function. As Leonard da Vinci's famous Proportions of the Human Figure (Virtruvian Man) illustrates, the size, shape, and proportions of the human body are defined by bones and their articulations. In this unit that focuses on the human skeleton, students are introduced to these concepts by asking them both to study da Vinci's drawing and build their own model of the human skeleton. Units on animal camouflage and architecture are also presented to further support the art and science connection.

Petto, Andrew; Petto, Sarah



The DaCHS Multi-protocol VO Server  

NASA Astrophysics Data System (ADS)

GAVO's Data Center Helper Suite (DaCHS) is a suite of tools for publishing data to the Virtual Observatory. It implements all major VO protocols (SCS, SIAP, SSAP, TAP, OAI-PMH). The integrated management and ingestion component allow defining metadata, structure, and services once and re-use the definition throughout the publication cycle from initial metadata aquisition to registry record generation. It has been driving GAVO's data center since 2008 and is now deployed in multiple locations around the globe. This poster briefly describes the design of the system as well as a bird's-eye view of data publishing with DaCHS.

Demleitner, M.



Mapeamento DA Vegetacao/USO DA Terra de Areas Indigenas Utilisando-Se Dados TM/LANDSAT (Mapping of Vegetation/Land Use of Indigenous Areas Utilizing TM/LANDSAT Data).  

National Technical Information Service (NTIS)

The objective of this work is to provide environmental information about indigenous areas (Ananas Ponta da Serra, Cajueiro, Ouro, Araca, Serra da Moca, Recanto da Saudade). The study area is located in Boa Vista, Bonfim, and Alto Alegre municipalities in ...

P. Hernandezfilho K. Defaria



A Run-time Environment for Da CaPo  

Microsoft Academic Search

It is a well known fact that the performance bottleneck of modern high speed networks is located in the end systems. Reasons are the complexity of the applied protocols and the embedding of protocols in the end systems. Da CaPo (D ynamic C onfigur ation of Pr otocols) provides an environment which allows the dynamic configuration of protocols with respect

M. Vogt; Bernhard Plattner; Thomas Plagemann; Thomas Walter




Microsoft Academic Search

o \\/h) e as matrizes de espécies por estação foram submetidas à análise de agrupamento e ordenação das amostras com base no índice de dissimilaridade de Bray-Curtis. Os dados indicaram a formação de distintas assembléias de espécies que representaram as diferentes regiões do talude ao longo da área de estudo. Variações significativas foram observadas entre médias e amplitudes de profundidade,



40 CFR 60.47Da - Commercial demonstration permit.  

Code of Federal Regulations, 2013 CFR

...Performance for Electric Utility Steam Generating Units § 60.47Da...for each 24-hour period of steam generator operation and to...following equivalent MW electrical generation capacity for any one equivalent MW electrical generation capacity for all...



How Computers shape Educational Activities at Casa da Música  

Microsoft Academic Search

In this paper we present the main ideas that shape musical experiences in the context of the Education Service program at Casa da Música, Porto and analyze how computers, applications and technology in general are part of a strategy that aims to provide a broad access to music making. A particular emphasis is given to technologies that have been purposely

P. M. Rodrigues; F. Lopes; A. P. Almeida; N. Peixoto; M. Mónica; J. Branco; P. J. Neto


Síndrome da cabeça caída em doença do neurônio motor  

Microsoft Academic Search

RESUMO - A síndrome da cabeça caída é causada por diminuição de força nos músculos extensores do pescoço sendo encontrada em diversas doenças neuro m u s c u l a res, bem como, na esclerose lateral amiotrófica. D e s c revemos o caso de três mulheres com diagnóstico de doença do neurônio motor com quadro clínico de disfagia

Paulo José Lorenzoni; Marcos Christiano Lange; Cláudia S. K. Kay; Luiz G. M. P. de Almeida; Hélio A. G. Teive; Rosana H. Scola; Lineu C. Werneck



Studying and Working Abroad. Leonardo da Vinci Series: Good Practices.  

ERIC Educational Resources Information Center

This document profiles recent successful examples of students studying and working abroad as part of the European Commission's Leonardo da Vinci program, which is designed to give students across the European Union the opportunity to experience vocational training in a foreign country. The following examples are presented: (1) 3 Finnish students…

Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.


MICROPROPAGAÇÃO DA AROEIRA (Myracrodruon urundeuva Fr. All) 1  

Microsoft Academic Search

RESUMO - A aroeira (Myracrodruon urundeuva Fr. All) é uma Anacardiaceae lenhosa que tem sido usada de forma predatória pela qualidade de sua madeira e pelo uso med i- cinal de sua casca desprovida de súber, por meio da qual se fabrica antiinflamatório. O objetivo deste trabalho foi o e s- tabelecimento \\



Vozes católicas no Congresso Nacional: aborto, defesa da vida  

Microsoft Academic Search

Este levantamento sobre os argumentos apresentados nos debates do Congresso Nacional faz parte de um trabalho mais amplo sobre a tramitação de projetos de lei relativos aos direitos sexuais e reprodutivos que tramitaram na década de 90. O aborto, um dos eventos da vida reprodutiva das mulheres, aparece na pauta dos debates mais polêmicos das últimas décadas, tanto em nível

Myriam Aldana




Microsoft Academic Search

Resumo No presente estudo foram aplicados os conceitos de Produção Mais Limpa no setor da serraria e de beneficiamento em uma madeireira, com o intuito de contribuir para a redução dos desperdícios de matéria-prima , água e energia; visando a integração deste setor industrial nos conceitos de Ecologia Industrial. Foi elaborado o seguinte objetivo geral: desenvolver uma proposta para a

Thompson Copperfield von Agner; Carlos Cezar Stadler


A fauna apícola do Parque Municipal da Cachoeirinha (Iporá, GO)  

Microsoft Academic Search

Bees are dependent on flower nectar and pollen (main sources of protein and energy, respectively) and have an important economic and ecological role as pollinators, representing about 40 to 90% of phanerogams pollinators in several ecosystems. This work presents a description of bee species from Parque Municipal da Cachoeirinha in Iporá, Goiás state, Brazil. A total of 58 hours of

Leandro Rodrigues Santiago; Rute Magalhães Brito; Thiago Mahlmann Vitoriano Lopes Muniz; Favízia Freitas de Oliveira; Flávio de Oliveira Francisco



A 16-bit 4'th order noise-shaping D\\/A converter  

Microsoft Academic Search

A 16-bit oversampling D\\/A (digital-to-analog) converter has been designed using a fourth-order all-digital noise-shaping loop followed by a 3-bit D\\/A converter. The 3-bit D\\/A converter, which uses a novel form of dynamic element matching achieves high accuracy and long-term stability without requiring precision matching of components. The harmonic distortion of the untrimmed monolithic CMOS prototype D\\/A converter is less than

L. Richard Carley; John Kenney



Yarkovsky-driven Impact Predictions: Apophis and 1950 DA  

NASA Astrophysics Data System (ADS)

Orbit determination for Near-Earth Asteroids presents unique technical challenges due to the imperative of early detection and careful assessment of the risk posed by specific Earth close approaches. The occurrence of an Earth impact can be decisively driven by the Yarkovsky effect, which is the most important nongravitational perturbation as it causes asteroids to undergo a secular variation in semimajor axis resulting in a quadratic effect in anomaly. We discuss the cases of (99942) Apophis and (29075) 1950 DA. The relevance of the Yarkovsky effect for Apophis is due to a scattering close approach in 2029 with minimum geocentric distance ~38000 km. For 1950 DA the influence of the Yarkovsky effect in 2880 is due to the long time interval preceding the impact. We use the available information from the astrometry and the asteroids' physical models and dynamical evolution as a starting point for a Monte Carlo method that allows us to measure how the Yarkovsky effect affects orbital predictions. We also find that 1950 DA has a 98% likelihood of being a retrograde rotator. For Apophis we map onto the 2029 close approach b-plane and analyze the keyholes corresponding to resonant close approaches. For 1950 DA we use the b-plane corresponding to the possible impact in 2880. We finally compute the impact probability from the mapped probability density function on the considered b-plane. For Apophis we have 4 in a million chances of an impact in 2068, while the probability of Earth impact in 2880 for 1950 DA is 0.04%.

Chesley, Steven R.; Farnocchia, D.; Chodas, P. W.; Milani, A.




Microsoft Academic Search

Resumo: Este artigo apresenta resultados de previsão da vazão afluente mensal do Rio São Francisco, que alimenta a usina de Sobradinho, usando-se redes neurais de Elman (RNA). As redes neurais de Elman utilizadas foram preparadas com o objetivo de receber como entrada amostras da série temporal da vazão afluente do Rio São Francisco deslocadas mês a mês. Para tal, nas

Luiz Biondi Neto; Pedro Henrique Gouvêa Coelho; Luís Chiganer; Lídia Angulo Meza; Luiz Palmeira Monteiro



First experiences with the da Vinci™ operating robot in thoracic surgery  

Microsoft Academic Search

Objectives: The da Vinci™ surgical robotic system was purchased at our institution in June 2001. The aim of this trial was to evaluate the applicability of the da Vinci™ operation robot for general thoracic procedures. Methods: The da Vinci™ surgical system consists of a console connected to a surgical arm cart, a manipulator unit with two instrument arms and a

J. Bodner; H. Wykypiel; G. Wetscher; T. Schmid



40 CFR 60.42Da - Standards for particulate matter (PM).  

Code of Federal Regulations, 2013 CFR

...false Standards for particulate matter (PM). 60.42Da Section 60.42Da Protection...42Da Standards for particulate matter (PM). (a) Except as provided in...March 1, 2005, any gases that contain PM in excess of 13 ng/J (0.03...



Impacto social da produção integrada de abacaxi no estado do Tocantins – Brasil  

Microsoft Academic Search

Além do objeto da produção em si, a produção integrada tem como princípios o bem-estar social e a preservação ambiental. Neste artigo avaliam-se os impactos sociais da produção integrada de abacaxi no Estado do Tocantins (Brasil) em oito estabelecimentos rurais. A ferramenta empregada na avaliação, denominada Ambitec Social, foi desenvolvida pela Embrapa, sob a liderança da Embrapa Meio Ambiente. O

Clovis Oliveira de Almeida; Aristoteles Pires de Matos; Carlos Estevao Leite Cardoso



Os determinantes da desigualdade de renda no Brasil: Luta de classes ou heterogeneidade educacional?  

Microsoft Academic Search

Este trabalho considera a questão: quais os principais determinantes da desigualdade de renda no Brasil? Após lembrar a importância da questão para a nossa economia, o artigo sugere um arcabouço genérico para categorizar os determinantes da distribuição de renda, baseado em cinco grupos de fatores: caraterísticas natas dos indivíduos; suas características adquiridas; o papel do mercado de trabalho; o papel

Francisco de Hollanda Guimarães Ferreira




Microsoft Academic Search

1 - INTRODUÇÃO A densidade da madeira é uma característica complexa resultante da combinação de diversos fatôres. Existem inúmeros trabalhos mostrando sua relação com as dimensões das fibras, particularmente espessura da parede, volume dos vasos e parênquimas, proporção entre madeira primaveril e outonal, e arranjo dos elementos anatômicos. Êste assunto tem sido intensivamente estudado para as coníferas, porém para as

Celso Edmundo; Bochetti Foelkel; Maria Aparecida Mourão Brasil; Luiz Ernesto; George Barrichelo


Application of DA-Preconditioned FINN for Electric Power System Fault Detection  

Microsoft Academic Search

This paper proposes a hybrid method of Deterministic Annealing (DA) and Fuzzy Inference Neural Network (FINN) for electric power system fault detection. It extracts features of input data with two-staged precondition of Fast Fourier Transform (FFT) and DA. FFT is useful for extracting the features of fault currents while DA plays a key role to classify input data into clusters

Tadahiro Itagaki; Hiroyuki Mori; Takeshi Yamada; Shoichi Urano



Novos Aspectos da Regulação do Trabalho no Brasil. Qual o Papel do Estado?  

Microsoft Academic Search

Uma inserção civilizada dos trabalhadores nos âmbitos econômico, social e político da vida brasileira depende da existência de relações assalariadas com direitos trabalhistas e previdenciários assegurados. Não obstante, ao longo dos anos 1990 e em parte dos atuais, os trabalhadores tenderam a assumir outras posições na estrutura de ocupações – posições quase sempre distintas da de assalariados formalmente registrados. Em

André Gambier Campos




Microsoft Academic Search

TABAI, K. C.; SALAY, E.; BARBOSA, C. G. Effects of a program for the analysis of food quality on consumer in the city of Campinas, São Paulo, Brazil. Revista de Ciências da Vida, Seropédica, RJ: EDUR, v. 29, n. 1, jan. - jun., p. 14-26, 2009. The Brazilian National Institute of Metrology & Industrial Quality (INMETRO) carries out a program




Microsoft Academic Search

They will be presented resulted gotten from the exe cuted digital mapping in the arenaceous pontal and in the dune field located in the beach of Atafona, São João da Barra (RJ), from geodesic surveys with GPS, in the ways relative and kinematic, with use of GTR-A\\/TechGeo dredges. Ahead of the ambient problem installed in this litt oral zone, next



Crescimento ponderal de filhotes de tartaruga gigante da Amazônia (Podocnemis expansa) submetidos a tratamento com rações isocalóricas contendo diferentes níveis de proteína bruta  

Microsoft Academic Search

RESUMO - A criação de Podocnemis expansa, a tartaruga-da-amazônia, com finalidade comercial foi implantada no Brasil em 1992. Contudo, muitas questões acerca do manejo, da nutrição, da sanidade e, principalmente, da reprodução ainda são obscuras. Visando contribuir para o desenvolvimento de uma metodologia alimentar da espécie, objetivou-se testar diferentes níveis de proteína da dieta. Uma amostra de 480 animais neonatos

Vinicius Augusto Sá; Leonardo C. Quintanilha; Gustavo Eduardo Freneau; Vera Lucia Ferreira Luz; Arcádio de Los Reyes Borja; Paulo César Silva



JCoDA: a tool for detecting evolutionary selection  

PubMed Central

Background The incorporation of annotated sequence information from multiple related species in commonly used databases (Ensembl, Flybase, Saccharomyces Genome Database, Wormbase, etc.) has increased dramatically over the last few years. This influx of information has provided a considerable amount of raw material for evaluation of evolutionary relationships. To aid in the process, we have developed JCoDA (Java Codon Delimited Alignment) as a simple-to-use visualization tool for the detection of site specific and regional positive/negative evolutionary selection amongst homologous coding sequences. Results JCoDA accepts user-inputted unaligned or pre-aligned coding sequences, performs a codon-delimited alignment using ClustalW, and determines the dN/dS calculations using PAML (Phylogenetic Analysis Using Maximum Likelihood, yn00 and codeml) in order to identify regions and sites under evolutionary selection. The JCoDA package includes a graphical interface for Phylip (Phylogeny Inference Package) to generate phylogenetic trees, manages formatting of all required file types, and streamlines passage of information between underlying programs. The raw data are output to user configurable graphs with sliding window options for straightforward visualization of pairwise or gene family comparisons. Additionally, codon-delimited alignments are output in a variety of common formats and all dN/dS calculations can be output in comma-separated value (CSV) format for downstream analysis. To illustrate the types of analyses that are facilitated by JCoDA, we have taken advantage of the well studied sex determination pathway in nematodes as well as the extensive sequence information available to identify genes under positive selection, examples of regional positive selection, and differences in selection based on the role of genes in the sex determination pathway. Conclusions JCoDA is a configurable, open source, user-friendly visualization tool for performing evolutionary analysis on homologous coding sequences. JCoDA can be used to rapidly screen for genes and regions of genes under selection using PAML. It can be freely downloaded at



The isolated massive DA white dwarf GD 50  

NASA Astrophysics Data System (ADS)

Analysis of accurate hydrogen line profiles from optical and ultraviolet spectrophotometry shows that the hot DA white dwarf GD 50 (WD 0346-011) has an exceptionally high surface gravity of log g = 9.0 + or - 0.15; the derived parameters imply a mass of 1.2 solar mass and a radius of 0.0057 solar mass, if an interior composed of carbon and oxygen is assumed. As such, it is the first well-studied, isolated DA white dwarf with a likely mass larger than that of Sirius B. Moreover, the derived mass is large enough to consider the possibility that the interior is composed of oxygen, neon, and magnesium. If GD 50 has evolved as a single object, it should be quite young. Alternatively, the star could have formed as the result of a merger of a close pair of white dwarfs.

Bergeron, P.; Kidder, K. M.; Holberg, J. B.; Liebert, James; Wesemael, F.; Saffer, Rex A.



The Da Vinci code dynamically de-coded.  


The novel The Da Vinci Code, by Dan Brown has been on best-seller lists for over two years. An examination of Brown's previous novels reveals a well-designed plot line shared by all four novels that not only makes them good "thrillers" but also creates a mythological structure to the novels that draws on common unconscious fantasies in the same way that fairy tales do. One aspect of this mythological structure is the use of evil conspiracies (and benign ones as well) for the protagonist to overcome. In addition, The Da Vinci Code presents a religious theme involving legends about Mary Magdalene. This theme touches on the role of a feminine aspect to divinity in allowing for an erotic connection with the divine. PMID:16448349

Cohen, Mariam




Microsoft Academic Search

1 RESUMO ñ Este trabalho teve por objetivo avaliar o desempenho de mudas da espØcie angico-branco (Anadenanthera colubrina (Vell.) Brenan), em condiÁies diferenciadas de disponibilidade de fÛsforo no solo e de saturaÁªo por bases. Os tratamentos foram representados por um fatorial de seis nÌveis de P (0, 100, 200, 300, 400 e 500 mg\\/dm 3 ) por cinco nÌveis de

Cristina de Oliveira Gomes; Haroldo Nogueira de Paiva; Lima Neves; Ricardo Silva



Improving Security in the ATLAS PanDA System  

NASA Astrophysics Data System (ADS)

The security challenges faced by users of the grid are considerably different to those faced in previous environments. The adoption of pilot jobs systems by LHC experiments has mitigated many of the problems associated with the inhomogeneities found on the grid and has greatly improved job reliability; however, pilot jobs systems themselves must then address many security issues, including the execution of multiple users' code under a common 'grid' identity. In this paper we describe the improvements and evolution of the security model in the ATLAS PanDA (Production and Distributed Analysis) system. We describe the security in the PanDA server which is in place to ensure that only authorized members of the VO are allowed to submit work into the system and that jobs are properly audited and monitored. We discuss the security in place between the pilot code itself and the PanDA server, ensuring that only properly authenticated workload is delivered to the pilot for execution. When the code to be executed is from a 'normal' ATLAS user, as opposed to the production system or other privileged actor, then the pilot may use an EGEE developed identity switching tool called gLExec. This changes the grid proxy available to the job and also switches the UNIX user identity to protect the privileges of the pilot code proxy. We describe the problems in using this system and how they are overcome. Finally, we discuss security drills which have been run using PanDA and show how these improved our operational security procedures.

Caballero, J.; Maeno, T.; Nilsson, P.; Stewart, G.; Potekhin, M.; Wenaus, T.




Microsoft Academic Search

RESUMO - Foi avaliada a ocorrŒncia e a distribuiÁªo de espØcies de fungos micorrÌzicos arbusculares (FMAs) e Acetobacter diazotrophicus em plantios de cana-de-aÁœcar em diferentes tipos de manejo nos Estados do Rio de Janeiro e Pernambuco. Foram feitas 35 coletas de amostras de solo da rizosfera e de raÌzes de 14 variedades de cana-de-aÁœcar para extraÁªo de esporos e isolamento



DaRT: A CALL System to Help Students Practice and Develop Reasoning Skills in Choosing English Articles.  

ERIC Educational Resources Information Center

Describes DaRT, a computer assisted language-learning system for helping English-as-a-Second-Language students master English articles. DaRT uses a diagrammatic reasoning tool to present communicative contexts for exercises in choosing appropriate articles. This paper describes the development of DaRT and DaRT's system components and concludes…

Yoshii, Rika; Milne, Alastair



Análise da medição do raio solar em ultravioleta  

NASA Astrophysics Data System (ADS)

A medição acurada do raio solar em qualquer banda do espectro eletromagnético é de relevância na formulação e calibração de modelos da estrutura e atmosfera solar. Esses modelos atribuem emissão do contínuo do Sol calmo em microondas à mesma região da linha Ha do Hell. Apresentamos a medição do raio solar em UV com imagens do EIT (Extreme Ultraviolet Image Telescope) entre 1996 e 2002, no comprimento de onda 30,9 nm (Ha do Hell), que se forma na região de transição/cromosfera solar. A técnica utilizada para o cálculo do raio UV foi baseada na transformada Wavelet B3spline. Fizemos um banco de dados com 1 imagem por dia durante o período citado. Obtivemos como resultado o raio médio da ordem de 975.61" e uma diminuição do mesmo para o período citado variando em média -0,45" /ano. Comparamos estes dados com os valores obtidos pelo ROI (Radio Observatório de Itapetinga) em 22/48 GHz e Nobeyama Radio Heliograph em 17 GHz mostrando que os raios médios são muito próximos o que indica que a região de formação nessas freqüências é a mesma conforme os modelos. Comparamos os resultados também com outros índices de atividade solar.

Saraiva, A. C. V.; Giménez de Castro, C. G.; Costa, J. E. R.; Selhorst, C. L.; Simões, P. J. A.



The 29-kDa proteins phosphorylated ion thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein  

SciTech Connect

Thrombin plays a critical role in platelet activation, hemostasis, and thrombosis. Cellular activation by thrombin leads to the phosphorylation of multiple proteins, most of which are unidentified. The authors have characterized several 29-kDa proteins that are rapidly phosphorylated following exposure of intact human platelets to thrombin. A murine monoclonal antibody raised to an unidentified estrogen receptor-related 29-kDa protein selectively recognized these proteins as well as a more basic, unphosphorylated 27-kDa protein. Cellular activation by thrombin led to a marked shift in the proportion of protein from the 27-kDa unphosphorylated form to the 29-kDa phosphoprotein species. Using this antibody, they isolated and sequenced a human cDNA clone encoding a protein that was identical to the mammalian 27-kDa heat shock protein (HSP27), a protein of uncertain function that is known to be phosphorylated to several forms and to be transcriptionally induced by estrogen. The 29-kDa proteins were confirmed to be phosphorylated forms of HSP27 by immunoprecipitation studies. Thus, the estrogen receptor-related protein is HSP27, and the three major 20-kDa proteins phosphorylated in thrombin-activated platelets are forms of HSP27. These data suggest a role for HSP27 in the signal transduction events of platelet activation.

Mendelsohn, M.E.; Yan Zhu; O'Neill, S. (Harvard Medical School, Boston, MA (United States))



Métodos de coleta de fezes e determinação dos coeficientes de digestibilidade da fração protéica e da energia de alimentos para o pacu, Piaractus mesopotamicus (Holmberg, 1887)  

Microsoft Academic Search

RESUMO - Para melhorar a alimentação do pacu (Piaracatus mesopotamicus), é necessário conhecer o nível de aproveitamento dos ingredientes utilizados em suas dietas. Neste trabalho, inicialmente, foram analisados quatro métodos de coleta de fezes, em função da precisão e da versatilidade (dissecação, extrusão, Guelph e Guelph modificado), e o tempo entre as coletas de fezes nos sistemas de Guelph, por

Eduardo Gianini Abimorad; Dalton José Carneiro



Purification and functional characterization of the 112 kDa adhesin of Entamoeba histolytica.  


The 112 kDa adhesin of E. histolytica is directly involved in the cytopathogenic activity of the parasite. We describe here the purification of the 112 kDa protein by electroelution and immunoaffinity chromatography using a monoclonal antibody against the adhesin. Two proteins of 70 and 50 kDa were eluted from the immunoaffinity column along with the 112 kDa adhesin. The three proteins were recognized by monospecific polyclonal antibodies against the adhesin. The same peptides (72 and 56 kDa) were also observed after incubation of the purified intact adhesin in diethylamine buffer. Proteins of 112 and 72 kDa were found to have protease activity, evidenced by their ability to degrade gelatin. Our results indicate that the 112 kDa adhesin was specifically broken down into two polypeptides of 50-56 and 70-72 kDa. The significance of this in vivo is as yet unclear. The adhesin has proteolytic activity, which is retained in the 70-72 kDa polypeptide but not in the 50-56 kDa one. PMID:1340303

Rigothier, M C; García-Rivera, G; Guaderrama, M; Orozco, E



Respostas religiosas ? aids no Brasil: impress?es de pesquisa acerca da Pastoral de DST/Aids da Igreja Cat?lica1  

PubMed Central

O texto encontra-se estruturado em quatro partes. Na primeira delas, apresentamos um conjunto de considerações e informações acerca da situação da aids no Brasil, das relações entre religião, sexualidade, aids e estado laico, bem como uma descrição mais clara do Projeto Respostas Religiosas ao HIV/Aids no Brasil, do qual este texto apresenta algumas impressões de pesquisa preliminares. A seguir, dedicamos um item a apresentação da Pastoral de DST/Aids, sua história, estrutura e objetivos. No item seguinte problematizamos diversas questões em particular no âmbito das relações Estado e Igreja, relações entre agentes de pastoral e hierarquia da Igreja, e questões ligadas mais diretamente à sexualidade e aids, todas referenciadas ao trabalho da Pastoral de DST/Aids. Ao final, apresentamos a bibliografia e fontes consultadas.

Seffner, Fernando; Silva, Cristiane Goncalves Meireles da; Maksud, Ivia; Garcia, Jonathan; Rios, Luis Felipe; Natividade, Marcelo; Borges, Priscila Rodrigues; Parker, Richard; Terto, Veriano



Evolução da morfologia de fases de blendas PA6\\/AES em extrusora de dupla rosca e moldagem por injeção  

Microsoft Academic Search

Resumo: A evolução da morfologia de fases em blendas não reativas e reativas de poliamida-6 com copolímero de acrilonitrila\\/EPDM\\/estireno (AES) em uma extrusora de rosca dupla co-rotacional foi investigada. A evolução da morfologia de fases ao longo da extrusora de rosca dupla foi monitorada através da coleta de pequenas amostras em válvulas no barril da extrusora e caracterização por microscopia

Adriane Bassani; Elias Hage Jr; Luiz A. Pessan; Ana V. Machado; José A. Covas



Reconstitution of surfactant activity by using the 6 kDa apoprotein associated with pulmonary surfactant.  

PubMed Central

Lipid extracts of bovine pulmonary surfactant containing the 6 kDa apoprotein, but lacking the 35 kDa apoprotein, can mimic the essential characteristics of pulmonary surfactant on a pulsating-bubble surfactometer. Reconstituted surfactant can be produced by combining silicic acid fractions containing 6 kDa apoprotein and phosphatidylglycerol with phosphatidylcholine. Treatment of the protein-containing fraction with proteolytic enzymes abolishes its efficacy. These results indicate that the presence of the 6 kDa apoprotein can account for some of the essential physical and biological characteristics of pulmonary surfactant. Immunodiffusion studies indicate that, contrary to earlier suggestions, the 6 kDa apoprotein is not structurally related to the major surfactant apoprotein that has a molecular mass of 35 kDa. Images Fig. 2. Fig. 3. Fig. 4.

Yu, S H; Possmayer, F



Mulheres de corpo e alma: aspectos biopsicossociais da meia-idade feminina  

Microsoft Academic Search

Resumo Este trabalho investigou na literatura cientÌfica os aspectos biolÛgicos, psicolÛgicos e socioculturais que se estªo em jogo na etapa da meia-idade feminina a fim de melhor compreender a vivŒncia dessa mulher. O evento da menopausa ñ caracterizada pela cessaÁªo da ovulaÁªo e por manifestaÁies fÌsicas e psÌquicas - marca este momento vital e impie questies que, se nªo forem

Maria Elizabeth Mori; Vera Lucia Decnop Coelho



Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)  

PubMed Central

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.

Sander, Jeffry D.; Dahlborg, Elizabeth J.; Goodwin, Mathew J.; Cade, Lindsay; Zhang, Feng; Cifuentes, Daniel; Curtin, Shaun J.; Blackburn, Jessica S.; Thibodeau-Beganny, Stacey; Qi, Yiping; Pierick, Christopher J.; Hoffman, Ellen; Maeder, Morgan L.; Khayter, Cyd; Reyon, Deepak; Dobbs, Drena; Langenau, David M.; Stupar, Robert M.; Giraldez, Antonio J.; Voytas, Daniel F.; Peterson, Randall T.; Yeh, Jing-Ruey J.; Joung, J. Keith



A 50-kDa variant form of human surfactant protein D  

Microsoft Academic Search

The dominant form of human surfactant protein D (SP-D) is a mul- timeric collagenous glycoprotein composed of monomeric subunits that have a molec- ular mass of 43 kDa under reducing conditions. However, in evaluating monoclonal antibodies to human SP-D, an additional monomeric subunit was identified with a reduced molecular mass of 50 kDa. This 50-kDa variant was detected in approximately

R. J. Mason; L. D. Nielsen; Y. Kuroki; E. Matsuura; J. H. Freed; J. M. Shannon



A 28-kDa cerebral neuropeptide from Manduca sexta: Relationship to the insect prothoracicotropic hormone  

Microsoft Academic Search

1.A 28-kDa peptide from the brain of the tobacco hornworm,Manduca sexta, was purifiedvia HPLC. The peptide copurified with the insect neurohormone, prothoracicotropic hormone (PTTH), through two HPLC columns.2.Immunocyctochemistry using polyclonal antibodies against the 28-kDa peptide revealed that the peptide was produced in the same protocerebral neurons that produce PTTH. Western blot analysis demonstrated that the 28-kDa peptide and big PTTH

Rosemary S. Gray; David P. Muehleisen; Eva J. Katahira; Walter E. Bollenbacher



Easy CD-DA Extractor 4.5.2  

NSDL National Science Digital Library

Billing itself as "the most complete CD-ripping solution available" as well as the fastest (a 40 percent increase in performance since the last edition), Easy CD-DA Extractor supports a variety of formats: mp3, Windows Media Player, Ogg Vorbis, TwinVQ, .wav, and .aiff. Using the program, you can copy CDs and convert from any of the supported formats (except .aiff) to any other. Users can define the length of the tracks they record; download in formation from the freedb, the Internet Compact Disc Database; normalize the music; and more. The program is shareware and costs $19.95.



Visual tracking of da Vinci instruments for laparoscopic surgery  

NASA Astrophysics Data System (ADS)

Intraoperative tracking of laparoscopic instruments is a prerequisite to realize further assistance functions. Since endoscopic images are always available, this sensor input can be used to localize the instruments without special devices or robot kinematics. In this paper, we present an image-based markerless 3D tracking of different da Vinci instruments in near real-time without an explicit model. The method is based on different visual cues to segment the instrument tip, calculates a tip point and uses a multiple object particle filter for tracking. The accuracy and robustness is evaluated with in vivo data.

Speidel, S.; Kuhn, E.; Bodenstedt, S.; Röhl, S.; Kenngott, H.; Müller-Stich, B.; Dillmann, R.



Chemical catalysis by the translocator protein (18 kDa).  


Translocator proteins (18 kDa) (TSPOs) are conserved integral membrane proteins. In both eukaryotes and prokaryotes, TSPOs interact with porphyrins, precursors of heme, and photosynthetic pigments. Here we demonstrate that bacterial TSPOs catalyze rapid porphyrin degradation in a light- and oxygen-dependent manner. The reaction is inhibited by a synthetic TSPO ligand PK11195 and by mutations of conserved residues, which affect either porphyrin binding or catalytic activity. We hypothesize that TSPOs are ancient enzymes mediating porphyrin catabolism with the consumption of reactive oxygen species. PMID:23651039

Ginter, Christopher; Kiburu, Irene; Boudker, Olga



Evolution of the ATLAS PanDA workload management system for exascale computational science  

NASA Astrophysics Data System (ADS)

An important foundation underlying the impressive success of data processing and analysis in the ATLAS experiment [1] at the LHC [2] is the Production and Distributed Analysis (PanDA) workload management system [3]. PanDA was designed specifically for ATLAS and proved to be highly successful in meeting all the distributed computing needs of the experiment. However, the core design of PanDA is not experiment specific. The PanDA workload management system is capable of meeting the needs of other data intensive scientific applications. Alpha-Magnetic Spectrometer [4], an astro-particle experiment on the International Space Station, and the Compact Muon Solenoid [5], an LHC experiment, have successfully evaluated PanDA and are pursuing its adoption. In this paper, a description of the new program of work to develop a generic version of PanDA will be given, as well as the progress in extending PanDA's capabilities to support supercomputers and clouds and to leverage intelligent networking. PanDA has demonstrated at a very large scale the value of automated dynamic brokering of diverse workloads across distributed computing resources. The next generation of PanDA will allow other data-intensive sciences and a wider exascale community employing a variety of computing platforms to benefit from ATLAS' experience and proven tools.

Maeno, T.; De, K.; Klimentov, A.; Nilsson, P.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.; Yu, D.; the Atlas Collaboration



SBS TDMA-DA system with VAC and DAC  

NASA Astrophysics Data System (ADS)

This paper describes many of the design considerations involved in developing the demand assignment (DA) subsystem of the TDMA satellite communication system for Satellite Business Systems. Each earth station has a satellite communications controller (SCC) which requests capacity to meet current voice and data traffic demands. A central reference station frequently reallocates capacity based on the requests of all the earth stations in a network. To minimize the amount of satellite capacity required, the SCC has a circuit-switching capability for voice calls as well as for digital data calls. Furthermore, the SCC employs voice activity compression (VAC) and data activity compression (DAC), in which the amount of capacity requested is based on measured average speech activity as well as the number of off-hook voice ports and the number of off-hook data ports. Data calls are queued on a first-come, first-served basis when capacity is not immediately available. The reference station distributes excess capacity according to a nonlinear table lookup procedure so that the voice call blocking probability is equalized across the network. The DA system makes much more efficient use of satellite transmission capacity than a design with fixed-capacity trunk routes.

Goode, B.



Metabonomics based NMR in Crohn's disease applying PLS-DA  

PubMed Central

Aim The aim of this study was to search for metabolic biomarkers of Crohn's disease (CD). Background Crohn's disease (CD) is a type of inflammatory bowel disease that causing a wide variety of symptoms. CD can influence any part of the gastrointestinal tract from mouth to anus. CD is not easily diagnosed because monitoring tools are currently insufficient. Thus, the discovery of proper methods is needed for early diagnosis of CD. Patients and methods We utilized metabolic profiling using proton nuclear magnetic resonance spectroscopy (1HNMR) to find the metabolites in serum. Classification of CD and healthy subject was done using partial least squares discriminant analysis (PLS-DA). Results According to PLS-DA model, we concluded that just using one descriptor CD and control groups could be classified separately. The level of lipid in blood serum of CD compared to healthy cohorts was decreased. For the external test set, the classification model showed a 94% correct classification of CD and healthy subject. Conclusion The result of classification model presents that NMR based metabonomics is key tool as well as insight into potential targets for disease therapy and prevention.

Fathi, Fariba; Oskouie, Afsaneh Arefi; Tafazzoli, Mohsen; Naderi, Nosratollah; Sohrabzedeh, Kaveh; Fathi, Soraya; Norouzinia, Mohsen



The 37kDa/67kDa Laminin Receptor acts as a receptor for A?42 internalization.  


Neuronal loss is a major neuropathological hallmark of Alzheimer's disease (AD). The associations between soluble A? oligomers and cellular components cause this neurotoxicity. The 37?kDa/67?kDa laminin receptor (LRP/LR) has recently been implicated in A? pathogenesis. In this study the mechanism underlying the pathological role of LRP/LR was elucidated. Försters Resonance Energy Transfer (FRET) revealed that LRP/LR and A? form a biologically relevant interaction. The ability of LRP/LR to form stable associations with endogenously shed A? was confirmed by pull down assays and A?-ELISAs. Antibody blockade of this association significantly lowered A?42 induced apoptosis. Furthermore, antibody blockade and shRNA mediated downregulation of LRP/LR significantly hampered A?42 internalization. These results suggest that LRP/LR is a receptor for A?42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide by facilitating intra-cellular A?42 accumulation. These findings recommend anti-LRP/LR specific antibodies and shRNAs as potential therapeutic tools for AD treatment. PMID:24990253

Da Costa Dias, Bianca; Jovanovic, Katarina; Gonsalves, Danielle; Moodley, Kiashanee; Reusch, Uwe; Knackmuss, Stefan; Weinberg, Marc S; Little, Melvyn; Weiss, Stefan F T



The 37kDa/67kDa Laminin Receptor acts as a receptor for A?42 internalization  

PubMed Central

Neuronal loss is a major neuropathological hallmark of Alzheimer's disease (AD). The associations between soluble A? oligomers and cellular components cause this neurotoxicity. The 37?kDa/67?kDa laminin receptor (LRP/LR) has recently been implicated in A? pathogenesis. In this study the mechanism underlying the pathological role of LRP/LR was elucidated. Försters Resonance Energy Transfer (FRET) revealed that LRP/LR and A? form a biologically relevant interaction. The ability of LRP/LR to form stable associations with endogenously shed A? was confirmed by pull down assays and A?-ELISAs. Antibody blockade of this association significantly lowered A?42 induced apoptosis. Furthermore, antibody blockade and shRNA mediated downregulation of LRP/LR significantly hampered A?42 internalization. These results suggest that LRP/LR is a receptor for A?42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide by facilitating intra-cellular A?42 accumulation. These findings recommend anti-LRP/LR specific antibodies and shRNAs as potential therapeutic tools for AD treatment.

Da Costa Dias, Bianca; Jovanovic, Katarina; Gonsalves, Danielle; Moodley, Kiashanee; Reusch, Uwe; Knackmuss, Stefan; Weinberg, Marc S.; Little, Melvyn; Weiss, Stefan F. T.



Identification of interaction domains of the prion protein with its 37-kDa/67-kDa laminin receptor  

PubMed Central

Cell-binding and internalization studies on neuronal and non-neuronal cells have demonstrated that the 37-kDa/67-kDa laminin receptor (LRP/LR) acts as the receptor for the cellular prion protein (PrP). Here we identify direct and heparan sulfate proteoglycan (HSPG)-dependent interaction sites mediating the binding of the cellular PrP to its receptor, which we demonstrated in vitro on recombinant proteins. Mapping analyses in the yeast two-hybrid system and cell-binding assays identified PrPLRPbd1 [amino acids (aa) 144–179] as a direct and PrPLRPbd2 (aa 53–93) as an indirect HSPG-dependent laminin receptor precursor (LRP)-binding site on PrP. The yeast two-hybrid system localized the direct PrP-binding domain on LRP between aa 161 and 179. Expression of an LRP mutant lacking the direct PrP-binding domain in wild-type and mutant HSPG-deficient Chinese hamster ovary cells by the Semliki Forest virus system demonstrates a second HSPG-dependent PrP-binding site on LRP. Considering the absence of LRP homodimerization and the direct and indirect LRP–PrP interaction sites, we propose a comprehensive model for the LRP–PrP–HSPG complex.

Hundt, Christoph; Peyrin, Jean-Michel; Haik, Stephane; Gauczynski, Sabine; Leucht, Christoph; Rieger, Roman; Riley, Maria Louise; Deslys, Jean-Philippe; Dormont, Dominique; Lasmezas, Corinne Ida; Weiss, Stefan




Microsoft Academic Search

Resumo: A preocupação com questões ambientais tem crescido nos últimos anos motivada, principalmente, pela busca da sociedade por conhecimentos que apontem amenização e soluções de problemas tanto em áreas urbanas como rurais. Diante desta preocupação e utilizando como base teórico-metodológica a Geomorfologia Ambiental, este trabalho apresenta um estudo geoambiental do curso médio da bacia hidrográfica do rio Verde, localizado no

Maristela Moresco Mezzomo; Andreia Quintão Soares


Effect of DA-9701 on Colorectal Distension-Induced Visceral Hypersensitivity in a Rat Model  

PubMed Central

Background/Aims DA-9701 is a newly developed drug made from the vegetal extracts of Pharbitidis semen and Co-rydalis tuber. The aim of this study was to evaluate the effect of DA-9701 on colorectal distension (CRD)-induced visceral hypersensitivity in a rat model. Methods Male Sprague-Dawley rats were subjected to neonatal colon irritation (CI) using CRD at 1 week after birth (CI group). At 6 weeks after birth, CRD was applied to these rats with a pressure of 20 to 90 mm Hg, and changes in the mean arterial pressure (MAP) were measured at baseline (i.e., without any drug administration) and after the administration of different doses of DA-9701. Results In the absence of DA-9701, the MAP changes after CRD were significantly higher in the CI group than in the control group at all applied pressures. In the control group, MAP changes after CRD were not significantly affected by the administration of DA-9701. In the CI group, however, the administration of DA-9701 resulted in a significant decrease in MAP changes after CRD. The administration of DA-9701 at a dose of 1.0 mg/kg produced a more significant decrease in MAP changes than the 0.3 mg/kg dose. Conclusions The administration of DA-9701 resulted in a significant increase in pain threshold in rats with CRD-induced visceral hypersensitivity.

Kim, Eun Ran; Min, Byung-Hoon; Lee, Tae Ho; Son, Miwon; Rhee, Poong-Lyul




Microsoft Academic Search

O complexo carnes é um dos principais componentes da pauta de exportação brasileira. O objetivo do artigo é analisar a evolução e a competitividade das exportações do complexo carnes brasileiro e os sub-complexos após 1990. Os procedimentos metodológicos envolvem a realização de uma revisão da literatura sobre competitividade e comércio internacional e coleta e tratamento de dados do complexo carnes

Luiz Gustavo Antonio De Souza; Vanderlei Jose Sereia; Marcia Regina Gabardo Camara; Marcia Goncalves Pizaia



O Papel da Certificação do Profissional de TI na Qualidade do Software: uma Investigação Preliminar  

Microsoft Academic Search

Resumo Desenvolver software com qualidade é muitas vezes considerado ilusório, dada a dificuldade de mensuração neste sentido. Além desta abordagem tradicional de determinar se o software é bom ou não, têm surgido outras abordagens alternativas para a determinação da qualidade do software, destacando-se hoje a determinação da maturidade do processo e o reconhecimento, via certificação, dos profissionais de desenvolvimento de

Alessandro Nunes de Souza; Edimara Mezzomo Luciano


Forma e funzioni delle reti di imprese: evidenze da una ricerca nel settore vitivinicolo  

Microsoft Academic Search

Il tema delle reti di imprese, già da tempo studiato, si presenta ora in tutta la sua rilevanza. La rete, infatti, costituisce uno strumento per incrementare la competitività delle imprese e per decretare il successo delle strategie da queste adottate. Il recente intervento che ha introdotto il contratto di rete testimonia anche il vivo interesse del legislatore. L’attuazione dello strumento

Federica Casarosa; Marco Gobbato



Tuning PEG-DA hydrogel properties via solvent-induced phase separation (SIPS)†  

PubMed Central

Poly(ethylene glycol) diacrylate (PEG-DA) hydrogels are widely utilized to probe cell-material interactions and ultimately for a material-guided approach to tissue regeneration. In this study, PEG-DA hydrogels were fabricated via solvent-induced phase separation (SIPS) to obtain hydrogels with a broader range of tunable physical properties including morphology (e.g. porosity), swelling and modulus (G?). In contrast to conventional PEG-DA hydrogels prepared from an aqueous precursor solution, the reported SIPS protocol utilized a dichloromethane (DCM) precursor solution which was sequentially photopolymerized, dried and hydrated. Physical properties were further tailored by varying the PEG-DA wt% concentration (5 wt%–25 wt%) and Mn (3.4k and 6k g mol ?1). SIPS produced PEG-DA hydrogels with a macroporous morphology as well as increased G? values versus the corresponding conventional PEG-DA hydrogels. Notably, since the total swelling was not significantly changed versus the corresponding conventional PEG-DA hydrogels, pairs or series of hydrogels represent scaffolds in which morphology and hydration or G? and hydration are uncoupled. In addition, PEG-DA hydrogels prepared via SIPS exhibited enhanced degradation rates.

Bailey, Brennan Margaret; Hui, Vivian; Fei, Ruochong



76 FR 19903 - Special Conditions: Diamond Aircraft Industry Model DA-40NG; Diesel Cycle Engine  

Federal Register 2010, 2011, 2012, 2013

...23-250-SC Special Conditions: Diamond Aircraft Industry Model DA-40NG; Diesel...conditions are issued for the Diamond Aircraft Industry (DAI) GmbH model DA-40NG the Austro Engine GmbH model E4 aircraft diesel engine (ADE) using...



Influência da Proximidade Geográfica na Dinâmica Inovativa de Firmas Localizadas em Sistemas Locais de Inovação  

Microsoft Academic Search

Propõe-se uma discussão a respeito da compreensão da dinâmica inovativa de firmas pertencentes a Sistemas Locais de Produção (SLPs). O objetivo é compreender a dinâmica inovativa de firmas localizadas em SLPs por meio das características dos fluxos de informação e conhecimento estabelecidos pelas firmas. Para tanto, foi realizada uma investigação de campo comparativa entre dois aglomerados do segmento produtor de

Janaína Ruffoni; Wilson Suzigan



Pa?u Ayurv?da (veterinary medicine) in Garudapur??a.  


The history of veterinary medicine is closely tied to the development of human medicine. Evidence of animal medicine has been found in ancient civilizations, such as those of the Hindu, Babylonians, Hebrews, Arabs, Greeks, and Romans. Ancient Indian literature in the form of the holy V?da, Pur?na, Br?hma?a, epics, etc. is flooded with information on animal care. The Pur??a are ancient scriptures discuss varied topics like devotion to God and his various aspects, traditional sciences like Ayurv?da, Jy?ti?a (Astrology), cosmology, concepts like dharma, karma, reincarnation and many others. The treatment of animal diseases using Ayurvedic medicine has been mentioned in Garudapur?na, Agnipur??a, Atri-samhit?, Matsyapur??a and many other texts. The Garudapur??a is one of the important S?ttvika pur?na, the subject matter is divided into two parts, viz. P?rvakha?da (first part) and an Uttarakha?da (subsequent part). Gav?yurv?da, Gaj?yurv?da narrated briefly and A?v?yurv?da described detailly in P?rvakha?da. PMID:19580108

Varanasi, Subhose; Narayana, A



Os direitos da mulher à terra e os movimentos sociais rurais na reforma agrária brasileira  

Microsoft Academic Search

R R R R Resumo: esumo: esumo: esumo: esumo: Este artigo examina a evolução da reivindicação dos direitos da mulher à terra na reforma agrária brasileira sob o prisma dos três principais movimentos sociais rurais: o Movimento dos Trabalhadores Rurais Sem Terra (MST), os sindicatos rurais e o movimento autônomo de mulheres rurais. O mérito maior por levantar a questão

Carmen Diana Deere



Associação movimento solidário colméia : um espaço para o resgate da cidadania das mulheres  

Microsoft Academic Search

Este trabalho tem como objetivo apresentar dados de uma investigação que o Grupo de Pesquisa Sexualidade e Escola (GESE) da Fundação Universidade Federal do Rio Grande (FURG) vem realizando junto às integrantes da Associação Movimento Solidário Colméia. Foram realizados vários encontros que funcionaram como um espaço narrativo em que as mulheres participaram ouvindo, contando e contrapondo histórias e fatos de

Guiomar Freitas Soares



Eunotiaceae (Bacillariophyceae) em igarapés da Amazônia Central, Manaus e Presidente Figueiredo, Brasil  

Microsoft Academic Search

RESUMO Estudos florísticos e taxonômicos envolvendo diatomáceas são escassos para a região amazônica. As publicações existentes incluem registros de diatomáceas da Amazônia brasileira, do Equador, da Colômbia e do Peru e comumente mostram que Eunotia e Actinella (Eunotiaceae) são gêneros bem representados nessa região. A maioria dos igarapés amazônicos costuma apresentar potencial hidrogeniônico (pH) ácido, característica aquática que promove o

Fernanda Ferrari; Letícia Knechtel Procopiak; Yamile Benaion Alencar; Thelma Alvim Veiga Ludwig




Microsoft Academic Search

Tendo em vista os recentes avanços da ciência, sobretudo na área de manipulação genética, a questão da clonagem humana surge como um dos grandes desafios a ser encarado. Nesse trabalho buscamos fazer uma análise não só jurídica, mas também técnica, ética e moral do tema, tendo como ponto de partida um estudo sobre a Bioética, passando pelo surgimento do Biodireito

Bernardo Abreu de Medeiros


A emergência da categoria “agricultor familiar” como sujeito de direitos na trajetória do sindicalismo rural brasileiro  

Microsoft Academic Search

O processo de reconhecimento dos agricultores familiares como sujeitos de direitos apesar de ser recente quando pensado a partir da trajetória do sindicalismo rural brasileiro demonstra ter suas primeiras raízes ainda na constituição da legislação trabalhista-sindical dos anos de 1930. Visando explorar esse processo o artigo tem por objetivo analisar a emergência dos agricultores familiares como sujeitos de direitos na

Everton Lazzaretti Picolotto



Impacto da redução dos encargos trabalhistas sobre a formalização e o salário das empregadas domésticas  

Microsoft Academic Search

O presente artigo faz uma avaliação da polarização de renda no Brasil para os anos de 1981 e 2003. Após diferenciar os conceitos de polarização e de desigualdade de renda, calculamos o índice de polarização da renda no Brasil, com base na metodologia proposta por Esteban, Gradin e Ray (1999). Os resultados indicam que a bipolarização se manteve praticamente constante

Maria Isabel Accoroni Theodoro; Luiz Guilherme Scorzafave



Compatibilizando Direitos Sociais com Competividade: Fiscais do Trabalho e a Implementação da Legislação Trabalhista no Brasil  

Microsoft Academic Search

Este estudo trata da seguinte questão: como compatibilizar direitos trabalhistas e proteção social com competitividade e produtividade das empresas? Na América Latina, há um intenso debate sobre a perversidade da legislação trabalhista (considerada prejudicial para os trabalhadores e para as empresas) e, em resposta, a política recomendada atualmente enfatiza a desregulamentação e a flexibilização dos direitos trabalhistas existentes. Em contraposição

Roberto Pires



DaRT: A Java Tool for Retrieving Archived Telescope Data  

NASA Astrophysics Data System (ADS)

DaRT is a client-side Java graphical user interface used for simultaneous retrieval of multiple documents and/or archived scientific instrument data sets which can be accessed via the World Wide Web. DaRT is currently used worldwide by astronomers to retrieve data sets from the BIMA Data Archive. DaRT can be configured to support data set retrieval from multiple data archives; an archivist can configure DaRT to support her archive simply by creating a short text file. Optionally, DaRT can support more features of an archive if more properties are added to this configuration file and/or by supplying an archive-specific Java class.

Mehringer, D. M.; Plante, R. L.


Changes in the pathogenicity of relapsed diminazene aceturate (DA)-resistant Trypanosoma brucei brucei as the trypanosomes are transmitted from DA-treated hosts to another set of animals  

Microsoft Academic Search

This study investigated the changes in pathogenicity of relapsed diminazene aceturate (DA)-resistant Trypanosoma brucei brucei (TBB) as the trypanosomes are transmitted from DA-treated hosts to another set of animals. The Federe strain of T. brucei brucei which was known to be DA resistant was used to infect two groups of rats, one of which was treated at the peak of

John Ikechukwu Ihedioha; Chinelo J. Onuma; Christian O. Okorie-Kanu; Thelma E. Ihedioha



Asteroseismology of pulsating DA white dwarfs with fully evolutionary models  

NASA Astrophysics Data System (ADS)

We present a new approach for asteroseismology of DA white dwarfs that consists in the employment of a large set of non-static, physically sound, fully evolutionary models representative of these stars. We already have applied this approach with success to pulsating PG1159 stars (GW Vir variables). Our white dwarf models, which cover a wide range of stellar masses, effective temperatures, and envelope thicknesses, are the result of fully evolutionary computations that take into account the complete history of the progenitor stars from the ZAMS. In particular, the models are characterized by self-consistent chemical structures from the centre to the surface, a crucial aspect of white dwarf asteroseismology. We apply this approach to an ensemble of 44 bright DAV (ZZ Ceti) stars.

Romero, A. D.; Córsico, A. H.; Althaus, L. G.; Miller Bertolami, M. M.



[Regarding the Manuscript D " Dell' occhio " of Leonardo da Vinci].  


Leonardo da Vinci's Manuscript D consists of five double pages sheets, which, folded in two, comprise ten folios. This document, in the old Tuscan dialect and mirror writing, reveals the ideas of Leonardo on the anatomy of the eye in relation to the formation of images and visual perception. Leonardo explains in particular the behavior of the rays in the eye in terms of refraction and reflection, and is very mechanistic in his conception of the eye and of the visual process. The most significant innovations found in these folios are the concept of the eye as a camera obscura and the intersection of light rays in the interior of the eye. His texts nevertheless show hesitation, doubts and a troubled confusion, reflecting the ideas and uncertainties of his era. He did not share his results in his lifetime, despite both printing and etching being readily available to him. PMID:19852385

Heitz, Robert F




SciTech Connect

We present a spectroscopically identified catalog of 70 DA white dwarfs (WDs) from the LAMOST pilot survey. Thirty-five are found to be new identifications after cross-correlation with the Eisenstein et al. and Villanova catalogs. The effective temperature and gravity of these WDs are estimated by Balmer lines fitting. Most of them are hot WDs. The cooling times and masses of these WDs are estimated by interpolation in theoretical evolution tracks. The peak of the mass distribution is found to be {approx}0.6 M {sub Sun }, which is consistent with prior work in the literature. The distances of these WDs are estimated using the method of synthetic spectral distances. All of these WDs are found to be in the Galactic disk from our analysis of space motions. Our sample supports the expectation that WDs with high mass are concentrated near the plane of the Galactic disk.

Zhao, J. K.; Luo, A. L.; Zhao, G. [Key Laboratory of Optical Astronomy, National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012 (China); Oswalt, T. D., E-mail:, E-mail:, E-mail:, E-mail: [Physics and Space Science Department, Florida Institute of Technology, Melbourne, FL 32901 (United States)



Discovery of Photospheric Germanium in Hot DA White Dwarfs  

NASA Astrophysics Data System (ADS)

We report the identification of Ge IV resonance lines in ultraviolet spectra of the hot DA white dwarfs Feige 24, G191-B2B, and GD 246. The lines originate in the stellar photosphere, and we measure low Ge/H abundance ratios ranging between -8.0 and -8.7. We also tentatively identify a resonance line of Sn IV blended with an Fe V line in the spectrum of G191-B2B. The presence of germanium extends our knowledge of the abundance pattern in hot white dwarfs beyond the iron group. The abundance ratio appears nearly solar, which implies either that the germanium abundance mixture in these stars has remained unaltered since leaving the main sequence or that diffusion processes (e.g., selective radiation pressure) are coincidentally reproducing a solar Ge/H ratio.

Vennes, Stéphane; Chayer, Pierre; Dupuis, Jean



Heavy-element abundance patterns in hot DA white dwarfs  

NASA Astrophysics Data System (ADS)

We present a series of systematic abundance measurements for 25 hot DA white dwarfs in the temperature range ~20 000-110 000 K, based on far-ultraviolet spectroscopy with the Space Telescope Imaging Spectrograph (STIS)/Goddard High Resolution Spectrograph (GHRS) on-board Hubble Space Telescope, IUE and FUSE. Using our latest heavy-element blanketed non-local thermodynamic equilibrium (non-LTE) stellar atmosphere calculations we have addressed the heavy-element abundance patterns, making completely objective measurements of abundance values and upper limits using a ?2 fitting technique to determine the uncertainties in the abundance measurements, which can be related to the formal upper limits in those stars where particular elements are not detected. We find that the presence or absence of heavy elements in the hot DA white dwarfs largely reflects what would be expected if radiative levitation is the supporting mechanism, although the measured abundances do not match the predicted values very well, as reported by other authors in the past. Almost all stars hotter than ~50 000 K contain heavy elements. For most of these the spread in element abundances is quite narrow and similar to the abundances measured in G191-B2B. However, there is an unexplained dichotomy at lower temperatures with some stars having apparently pure H envelopes and others having detectable quantities of heavy elements. The heavy elements present in these cooler stars are often stratified, lying in the outermost layers of the envelope. A few strong temperature/evolutionary effects are seen in the abundance measurements. There is a decreasing Si abundance with temperature, the N abundance pattern splits into two groups at lower temperature and there is a sharp decline in Fe and Ni abundance to zero, below ~50 000 K. When detected, the Fe and Ni abundances maintain an approximately constant ratio, close to the cosmic value of ~20. For the hottest white dwarfs observed by STIS, the strongest determinant of abundance appears to be gravity.

Barstow, M. A.; Good, S. A.; Holberg, J. B.; Hubeny, I.; Bannister, N. P.; Bruhweiler, F. C.; Burleigh, M. R.; Napiwotzki, R.



A estabilidade dos PAHS em função da energia da radiação interestelar nas faixas UV e raios-X  

NASA Astrophysics Data System (ADS)

A nebulosa CRL 618, uma proto-nebulosa planetária cuja nuvem molecular espessa envolve uma estrela B0, contém uma grande quantidade de C2H2 e CH4. Estas moléculas são consideradas os tijolos da criação de grandes moléculas carbonadas como os Hidrocarbonetos Policíclicos Aromáticos (PAHs). Esta nebulosa, por estar exposta a intensos campos de UV e Raios-X, é uma região de fotodissociação molecular que propicia a formação de novas moléculas, confirmada pela presença de C4H2 e C6H6 (Benzeno), que é a unidade básica dos PAHs. Atribui-se a esta família de moléculas orgânicas duas propriedades fundamentais, a resistência para sobreviver ao campo de radiação UV interestelar e a geração das bandas de emissão não identificadas (UIR) observadas no infravermelho. No entanto, alguns autores questionam a resistência dos PAHs ao campo de radiação UV interestelar. Empregando a técnica de Espectrometria de Massas por Tempo de Vôo, no modo de coincidência fotoelétron-fotoíon, estudamos a ionização e fragmentação das seguintes moléculas: Benzeno, Benzeno deuterado, Naftaleno, Antraceno e Fenantreno. Utilizamos uma fonte de Hélio monocromática em 21,21 eV (584,5 Å) e a radiação Síncroton do Laboratório Nacional de Luz Síncroton (LNLS) em diferentes energias nas proximidades da borda do C 1s ( 290 eV). Comprovamos a estabilidade dos PAHs sob ação de UV (21,21 eV), onde eles apresentam um baixo nível de fotodissociação, produzindo fragmentos ionizados com rendimento total na ordem de 5 por cento em relação ao íon molecular pai. Entretanto, em altas energias, na faixa de Raios-X, a quebra destas moléculas torna-se mais intensa, com a produção de muitos fragmentos. Como uma das rotas de fragmentação do Naftaleno é [(C10H8) = > (C6H6+) + (C4H2) + (e-)], e como temos as evidências observacionais da existência do C4H2 e C6H6 na nebulosa CRL 618, sugerimos que este ambiente também possui o Naftaleno.

Pinotti, R.; Costa, R. K.; Boechat-Roberty, H. M.; Lago, A.; Souza, G. B.




Microsoft Academic Search

SANTOS, I. B. dos; MIRANDA, L. H. M. de; OKAMOTO, T.; FIGUEIREDO, F. B.; SCHUBACH, T. M. P.; LEME, L. R. P.; QUINTELLA, L. P.; TORTELLY, R. American tegumentary leishmaniose in dogs from Rio de Janeiro, review. Revista Universidade Rural: Série Ciências da Vida, Seropédica, RJ: EDUR, v. 28 n 1, p. 27-38, jan-juh, 2008. American tegumentary leishmaniasis in dogs




Aspiração de corpo estranho na árvore traqueobrônquica em crianças: avaliação de seqüelas através de exame cintilográfico  

Microsoft Academic Search

Sequelae of foreign body aspiration in the respiratory tract of children Objective: The aim of this study was to detect late sequelae of foreign body aspiration in the respiratory tract of children. Methods: This study included children admitted from 1987 to 1999 to Hospital da Criança Santo Antônio, of Porto Alegre, with clinical symptoms of foreign body aspiration to the




[Bacteriological quality of Bendetti Lake (Santo Tomé, Santa Fe Province, Argentina) and associated environmental variables].  


The main objective was to determine the bacteriological quality of a shallow lake in the lower Salado River Basin (Bedetti, 31 degrees 40' S and 60 degrees 45' W). Seventy samples were tested by the most probable number (MPN) method, throughout three years. The number of thermotolerant coliforms was very variable: from 20 MPN/100 ml to 130,000 MPN/100 ml. These variations were mainly associated to the local rains (rs = 0.4853, p = 0.0001) and to the hydrometric level-h-(rs = 0.3835, p = 0.008). Statistically significant correlations between coliform bacteria and other variables (temperature, oxygen, pH, conductivity and transparency) were not found. The increase of coliforms associated to the increase of the hydrometric level indicates the presence of diffuse contamination sources. It was found that the rains affected the bacteriological quality, specially during the low water periods (h < 3.60). During the high water periods (h > 4.70) one of the main coliform contributor was represented by the incoming floating islans and aquatic macrophyte, from allochthonous fluvial systems. The coliform concentrations between the Bedetti lake and the Salado river were compared. The results show a better bacteriological quality of the lake (geometric average = 1,990/100 ml vs. 420/100 ml, n = 56) so that, connecting them by the construction of an artificial channel is not advisable. PMID:9629605

Emiliani, F; González de Paira, S M



HIV aspartyl protease inhibitors as promising compounds against Candida albicans Andr? Luis Souza dos Santos  

PubMed Central

Cells of Candida albicans (C. albicans) can invade humans and may lead to mucosal and skin infections or to deep-seated mycoses of almost all inner organs, especially in immunocompromised patients. In this context, both the host immune status and the ability of C. albicans to modulate the expression of its virulence factors are relevant aspects that drive the candidal susceptibility or resistance; in this last case, culminating in the establishment of successful infection known as candidiasis. C. albicans possesses a potent armamentarium consisting of several virulence molecules that help the fungal cells to escape of the host immune responses. There is no doubt that the secretion of aspartyl-type proteases, designated as Saps, are one of the major virulence attributes produced by C. albicans cells, since these hydrolytic enzymes participate in a wide range of fungal physiological processes as well as in different facets of the fungal-host interactions. For these reasons, Saps clearly hold promise as new potential drug targets. Corroborating this hypothesis, the introduction of new anti-human immunodeficiency virus drugs of the aspartyl protease inhibitor-type (HIV PIs) have emerged as new agents for the inhibition of Saps. The introduction of HIV PIs has revolutionized the treatment of HIV disease, reducing opportunistic infections, especially candidiasis. The attenuation of candidal infections in HIV-infected individuals might not solely have resulted from improved immunological status, but also as a result of direct inhibition of C. albicans Saps. In this article, we review updates on the beneficial effects of HIV PIs against the human fungal pathogen C. albicans, focusing on the effects of these compounds on Sap activity, growth behavior, morphological architecture, cellular differentiation, fungal adhesion to animal cells and abiotic materials, modulation of virulence factors, experimental candidiasis infection, and their synergistic actions with classical antifungal agents.

dos Santos, Andre Luis Souza



Assessing human–dog conflicts in Todos Santos, Guatemala: Bite incidences and public perception  

Microsoft Academic Search

The issues surrounding dog bites are a major public health concern, particularly in areas of low income where accessibility to adequate health care, veterinary medicine and sufficient management of canine population control is low. An understanding of the risk factors associated with human–dog conflicts may be important when establishing dog bite and disease prevention strategies. In May 2008, a census

Meg Lunney; Andria Jones; Enid Stiles; David Waltner-Toews



A Hospital-based Survey of BCG Coverage in Santo Domingo, the Dominican Republic1  

Microsoft Academic Search

A cross-sectional study was conducted in April-June 1992 at the outpatient clinic of the Roberf Reid Children's Hospifal in Sanfo Domingo to assess BCG vaccination coverage and facfors affecting BCG vaccination of children. A consecutive sample of 2 000 patients under 18 years of age was included in the study. The person accompanying the patient to the hospital was interviewed,



Groundwater Resources of Ribeira Paul Basin, Island of Santo Antao, Cape Verde, West Africa  

USGS Publications Warehouse

Groundwater resources in Cape Verde provide water for agriculture, industry, and human consumption. These resources are limited and susceptible to contamination. Additional groundwater resources are needed for continued agricultural development, particularly during times of drought, but increased use and (or) climatic change may have adverse effects on the quantity and quality of freshwater available. In volcanic island aquifers such as those of Cape Verde, a lens of fresh groundwater typically ?floats? upon a layer of brackish water at the freshwater/saltwater boundary, and increased pumping may cause salt water intrusion or other contamination. A recent U.S. Geological Survey study assessed baseline groundwater conditions in watersheds on three islands of Cape Verde to provide the scientific basis for sustainably developing water resources and minimizing future groundwater depletion and contamination.

Heilweil, Victor M.; Gingerich, Stephen B.; Verstraeten, Ingrid M.



Avanços no entendimento da base genética de doenças hered- itárias monogênicas da barreira epidérmica: novas pistas para os principais genes que podem estar envolvidos na patogênese da dermatite atópica.* Advances in understanding the genetic basis of inherited single gene skin barrier disorders: new clues to key genes that may be involved in the pathogenesis of atopic dermatitis  

Microsoft Academic Search

2 Resumo: O maior conhecimento sobre as de sequências genômicas de DNA e as bases de dados gené- ticas levou à caracterização da base molecular de várias doenças hereditárias de pele. Nesta revisão resumimos algumas das descobertas recentes mais importantes quanto à definição das mutações patogênicas que causam as doenças hereditárias da barreira cutânea, levando a descamação ou aumento da

Joey E Lai-Cheong; John A. McGrath


Endogenous dopamine (DA) modulates (3H)spiperone binding in vivo in rat brain  

SciTech Connect

(3H)spiperone (SPI) binding in vivo, biochemical parameters and behavior were measured after modulating DA levels by various drug treatments. DA releasers and uptake inhibitors increased SPI binding in rat striatum. In other brain areas, the effects were variable, but only the pituitary remained unaffected. Surprisingly, nomifensine decreased SPI binding in frontal cortex. The effects of these drugs were monitored by measuring DA, serotonin (5-HT) and their metabolites in the same rats. The increased SPI binding in striatum was parallel to the locomotor stimulation with the following rank order: amfonelic acid greater than nomifensine greater than D-amphetamine greater than or equal to methylphenidate greater than amineptine greater than bupropion. Decreasing DA levels with reserpine or alpha-methyl-para-tyrosine reduced SPI binding by 45% in striatum only when both drugs were combined. In contrast, reserpine enhanced SPI binding in pituitary. Thus, the amount of releasable DA seems to modulate SPI binding characteristics. It is suggested that in vivo, DA receptors are submitted to dynamic regulation in response to changes in intrasynaptic concentrations of DA.

Bischoff, S.; Krauss, J.; Grunenwald, C.; Gunst, F.; Heinrich, M.; Schaub, M.; Stoecklin, K.V.; Vassout, A.; Waldmeier, P.; Maitre, L. (Research Department, CIBA-GEIGY Ltd., Basel (Switzerland))



Characterization of a 95 kDa high affinity human high density lipoprotein-binding protein.  


A new human 95 kDa high density lipoprotein (HDL)-binding protein (HBP) corresponding to a high affinity HDL-binding site with K(d) = 1.67 microg/mL and a capacity of 13.4 ng/mg was identified in human fetal hepatocytes. The HDL binding with the 95 kDa HBP plateaus at 2.5-5 microg/mL under reducing and nonreducing conditions. The association of HDL(3) with the 95 kDa HBP plateaued in 15-30 min while dissociation was complete in 30 min. HDL(3), apoA-I, and apoA-II were recognized by the 95 kDa HBP while low density lipoproteins (LDL) and tetranitromethane-modified HDL were not. The 95 kDa HBP predominantly resides on the surface of cells since trypsin treatment of HepG2 cells eliminated nearly 70% of HDL binding. All studied human cells and cell lines (HepG2, Caco-2, HeLa, fibroblasts, SKOV-3, PA-I) demonstrated the presence of the 95 kDa protein. Both RT-PCR and Western blotting for HB-2/ALCAM were negative in human fetal hepatocytes while Gp96/GRP94 was clearly differentiated from the 95 kDa HBP by two-dimensional electrophoretic mobility. Moreover, deglycosylation of HepG2 membrane preparations did not affect either HDL binding to the 95 kDa HBP or its size, while in contrast it affected the molecular weights of HB-2/ALCAM and SR-BI/CLA-1. We conclude that the 95 kDa HBP is a new HDL receptor candidate widely expressed in human cells and cell lines. PMID:11284697

Bocharov, A V; Vishnyakova, T G; Baranova, I N; Patterson, A P; Eggerman, T L



High resolution structure of cleaved Serpin 42 Da from Drosophila melanogaster  

PubMed Central

Background The Drosophila melanogaster Serpin 42 Da gene (previously Serpin 4) encodes a serine protease inhibitor that is capable of remarkable functional diversity through the alternative splicing of four different reactive centre loop exons. Eight protein isoforms of Serpin 42 Da have been identified to date, targeting the protease inhibitor to both different proteases and cellular locations. Biochemical and genetic studies suggest that Serpin 42 Da inhibits target proteases through the classical serpin ‘suicide’ inhibition mechanism, however the crystal structure of a representative Serpin 42 Da isoform remains to be determined. Results We report two high-resolution crystal structures of Serpin 42 Da representing the A/B isoforms in the cleaved conformation, belonging to two different space-groups and diffracting to 1.7 Å and 1.8 Å. Structural analysis reveals the archetypal serpin fold, with the major elements of secondary structure displaying significant homology to the vertebrate serpin, neuroserpin. Key residues known to have central roles in the serpin inhibitory mechanism are conserved in both the hinge and shutter regions of Serpin 42 Da. Furthermore, these structures identify important conserved interactions that appear to be of crucial importance in allowing the Serpin 42 Da fold to act as a versatile template for multiple reactive centre loops that have different sequences and protease specificities. Conclusions In combination with previous biochemical and genetic studies, these structures confirm for the first time that the Serpin 42 Da isoforms are typical inhibitory serpin family members with the conserved serpin fold and inhibitory mechanism. Additionally, these data reveal the remarkable structural plasticity of serpins, whereby the basic fold is harnessed as a template for inhibition of a large spectrum of proteases by reactive centre loop exon ‘switching’. This is the first structure of a Drosophila serpin reported to date, and will provide a platform for future mutational studies in Drosophila to ascertain the functional role of each of the Serpin 42 Da isoforms.



Application of DA-Preconditioned FINN for Electric Power System Fault Detection  

NASA Astrophysics Data System (ADS)

This paper proposes a hybrid method of Deterministic Annealing (DA) and Fuzzy Inference Neural Network (FINN) for electric power system fault detection. It extracts features of input data with two-staged precondition of Fast Fourier Transform (FFT) and DA. FFT is useful for extracting the features of fault currents while DA plays a key role to classify input data into clusters in a sense of global classification. FINN is a more accurate estimation model than the conventional artificial neural networks (ANNs). The proposed method is successfully applied to data obtained by the Tokyo Electric Power Company (TEPCO) power simulator.

Itagaki, Tadahiro; Mori, Hiroyuki; Yamada, Takeshi; Urano, Shoichi


2012 DA14 and Chelyabinsk: Same Day Coincidence?  

NASA Astrophysics Data System (ADS)

A long anticipated near-miss by near-Earth asteroid (NEA) 2012 DA14 on 15 February 2013 was upstaged 16 hours earlier by the impact and atmospheric explosion of a ~20 m NEA over Chelyabinsk, Russia. DA14 was earlier estimated to be 45 m across and passage at a distance under geosynchronous satellites was considered to be a record close approach by an object of that size, a once-in-40-years event. Actual Earth impact by a 20 m NEA had been estimated to be a once-in-200-years event. A simplistic calculation gives a probability of these happening on the same day of 1-in-a-billion. Within hours of the Chelyabinsk impact, it was recognized and widely reported that the two asteroids were in very different orbits and could not, at least in any readily understandable way, be physically related to each other (e.g. fragments of the same precursor body). Also, some early reports suggested that the two asteroids had very different compositions, further undermining the possibility they were pieces of the same body. (It seems unlikely, though certainly conceivable, that the two NEAs could have different compositions yet have some kind of causal connection resulting in the same-day events.) Nevertheless, incredibly tiny probabilities beg for an explanation. Here, with the benefit of hindsight, I re-examine issues that affect the probabilities, such as size, albedo, probable composition, and numbers of NEAs of these sizes. I also consider difficult issues of framing the apparent coincidence, which dominate other uncertainties. Updated information about the physical nature of the two asteroids somewhat modifies the original estimates of probabilities. Moreover, more proper ways of framing elements of the coincidence considerably reduce the improbability of the same-day events, but not nearly to the level (e.g. 1-in-1000 or perhaps 1-in-10,000) where we could rationalize dismissing the events as “just a coincidence.” The events of 15 February 2013 deserve more sophisticated investigation.

Chapman, Clark R.




Microsoft Academic Search

Glial evolution still remains unknown, although this process should had required adaptations to the internal medium of the nervous system and to functional needs in the neuroaxis of each species. The hypothesis of the functional and adaptive importance of glia, based on the astrocytary functional multiplicity is supported here as the main objective. The number of glial cells increases in



Abordaje clínico de las disfunciones sexuales femeninas: perspectiva orgánica  

Microsoft Academic Search

What should be really categorized as female sexual dysfunction (FSD) is under debate, although there is agreement in its relatively high prevalence. The multidimensional and contextual nature of female sexual expression is also acknowledged, and that must be kept in mind when addressing FSD. It is also recognized that it relies on a biological support which can deteriorate by several

Manuel Mas; Delia Báez




Microsoft Academic Search

El presente ensayo analiza las principales teorias evolutivas desde Darwin hasta la fecha, entre ellas la teoría de Lamark y la Teoría de la Quimiosintética, todas ellas con un sesgo fuertemente biologico, donde los cambios más decisivos en la homnización que se observan son: la adquisición de la postura erecta, que liberó sus extremidades anteriores y el desarrollo del cerebro,

Fredy Hardy Wompner Gallardo




Microsoft Academic Search

This line considers three aims: 1. to identify the dimensions (structure factor) of the physical, personal and social self-concepts and to measure them (constructing measurement instruments); 2. to identify the variability of self-concept domains and dimensions in relation to factors such as age, gender, and social and personal facets, which, in turn, will enable us to verify the validity of

Igor Esnaola; Alfredo Goñi; José María Madariaga




Microsoft Academic Search

The discovery of molecular biology's secrets has marked a turning point in biological sciences. Genomics constitutes a fundamental part of this progress and it is defined as http:\\/\\/

Esteban R. Arrieta Bolaños


Neosporosis bovina: conceptos generales, inmunidad y perspectivas para la vacunación  

Microsoft Academic Search

SUMMARY Bovine neosporosis: general concepts, immunity and perspectives for vaccination. Neospora caninum causes abortions in cattle worldwide. The Neospora-cycle of life is heteroxenous. Dogs (Canis familiaris) and coyotes (Canis latrans) are the definitive hosts known at present. Although, transplacental infection is an efficiently mode of trans- mission in cattle; there are also experimental and field data that prove horizontal transmission.



Immigration Reform and Related Issues. Perspectivas Publicas. Issue Update.  

ERIC Educational Resources Information Center

Asserting that immigration reform and related issues have commanded sustained attention in the opening weeks of the 99th Congress, this paper provides an overview of important developments in this area, and highlights steps taken by the National Council of La Raza to help shape these developments. The developments discussed include: (1) The…

National Council of La Raza, Washington, DC.


Near-UV Absorption in Very Cool DA White Dwarfs  

NASA Astrophysics Data System (ADS)

The atmospheres of very cool, hydrogen-rich white dwarfs (WDs) (T eff < 6000 K) are challenging to model because of the increased complexity of the equation of state, chemical equilibrium, and opacity sources in a low-temperature, weakly ionized dense gas. In particular, many models that assume relatively simple models for the broadening of atomic levels and mostly ideal gas physics overestimate the flux in the blue part of their spectra. A solution to this problem that has met with some success is that additional opacity at short wavelengths comes for the extreme broadening of the Lyman ? line of atomic H by collisions primarily with H2. For the purpose of validating this model more rigorously, we acquired Hubble Space Telescope STIS spectra of eight very cool WDs (five DA and three DC stars). Combined with their known parallaxes, BVRIJHK, and Spitzer IRAC photometry, we analyze their entire spectral energy distribution (from 0.24 to 9.3 ?m) with a large grid of model atmospheres and synthetic spectra. We find that the red wing of the Lyman ? line reproduces the rapidly decreasing near-UV flux of these very cool stars very well. We determine better constrained values of T eff and gravity as well as upper limits to the helium abundance in their atmospheres.

Saumon, D.; Holberg, J. B.; Kowalski, P. M.



Audio annotation watermarking with robustness against DA/AD conversion  

NASA Astrophysics Data System (ADS)

In the paper we present a watermarking scheme developed to meet the specific requirements of audio annotation watermarking robust against DA/AD conversion (watermark detection after playback by loudspeaker and recording with a microphone). Additionally the described approach tries to achieve a comparably low detection complexity, so it could be embedded in the near future in low-end devices (e.g. mobile phones or other portable devices). We assume in the field of annotation watermarking that there is no specific motivation for attackers to the developed scheme. The basic idea for the watermark generation and embedding scheme is to combine traditional frequency domain spread spectrum watermarking with psychoacoustic modeling to guarantee transparency and alphabet substitution to improve the robustness. The synchronization and extraction scheme is designed to be much less computational complex than the embedder. The performance of the scheme is evaluated in the aspects of transparency, robustness, complexity and capacity. The tests reveals that 44% out of 375 tested audio files pass the simulation test for robustness, while the most appropriate category shows even 100% robustness. Additionally the introduced prototype shows an averge transparency of -1.69 in SDG, while at the same time having a capacity satisfactory to the chosen application scenario.

Qian, Kun; Kraetzer, Christian; Biermann, Michael; Dittmann, Jana




SciTech Connect

A total of {approx}640, 000 objects from the LAMOST pilot survey have been publicly released. In this work, we present a catalog of DA white dwarfs (DAWDs) from the entire pilot survey. We outline a new algorithm for the selection of white dwarfs (WDs) by fitting Sersic profiles to the Balmer H{beta}, H{gamma}, and H{delta} lines of the spectra, and calculating the equivalent width of the Ca II K line. Two thousand nine hundred sixty-four candidates are selected by constraining the fitting parameters and the equivalent width of the Ca II K line. All the spectra of candidates are visually inspected. We identify 230 DAWDs (59 of which are already included in the Villanova and SDSS WD catalogs), 20 of which are DAWDs with non-degenerate companions. In addition, 128 candidates are classified as DAWDs/subdwarfs, which means the classifications are ambiguous. The result is consistent with the expected DAWD number estimated based on the LEGUE target selection algorithm.

Zhang Yueyang; Deng Licai; Liu Chao; Carrell, Kenneth; Yang Fan; Gao Shuang; Xu Yan; Li Jing; Zhang Haotong; Zhao Yongheng; Luo Ali; Bai Zhongrui; Yuan Hailong [Key Lab for Optical Astronomy, National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012 (China); Lepine, Sebastien [Department of Astrophysics, Division of Physical Sciences, American Museum of Natural History, Central Park West at 79th Street, New York, NY (United States); Newberg, Heidi Jo; Carlin, Jeffrey L. [Department of Physics, Applied Physics and Astronomy, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180 (United States); Jin Ge [University of Science and Technology of China, Hefei 230026 (China)



First results from the FINUDA experiment at DA?NE  

NASA Astrophysics Data System (ADS)

In spring 2003 the FINUDA detector was installed at the ? factory DA?NE in the Laboratori Nazionali di Frascati of INFN (Italy). In October 2003 the commissioning of the apparatus was accomplished and the first data taking started with a set of nuclear targets 6Li, 7Li, natC, 27Al, 51V. The data collection will continue until a total integrated luminosity of 250 pb is recorded. Light and medium A hypernuclei will be abundantly produced by the strangeness exchange reaction induced by the stopped K coming from the decay of ?(1020) mesons. The aim of the experiment is to simultaneously measure the excitation energy spectra of the produced hypernuclei, with a resolution better than 1 MeV, the lifetime of the ? embedded in the different hypernuclei and the partial widths ?, ? and ? for mesonic and non-mesonic hypernuclear decays. Information on neutron-rich hypernuclei and rare hypernuclear two-body decays might be available too, with the statistics that will be collected at the end of the run. In the present paper, first results concerning in-beam detector calibration, spectrometer performances and very preliminary hypernuclear formation and decay spectra will be presented.

Agnello, M.; Beer, G.; Benussi, L.; Bertani, M.; Bianco, S.; Botta, E.; Bressani, T.; Busso, L.; Calvo, D.; Camerini, P.; Cerello, P.; Dalena, B.; De Mori, F.; D'Erasmo, G.; Di Santo, D.; Fabbri, F. L.; Faso, D.; Feliciello, A.; Filippi, A.; Filippini, V.; Fiore, E. M.; Fujioka, H.; Gianotti, P.; Grion, N.; Krasnoperov, A.; Lucherini, V.; Marcello, S.; Maruta, T.; Mirfakhrai, N.; Morra, O.; Olin, A.; Pace, E.; Palomba, M.; Pantaleo, A.; Panzarasa, A.; Paticchio, V.; Piano, S.; Pompili, F.; Rui, R.; Simonetti, G.; So, H.; Tomassini, S.; Wheadon, R.; Zenoni, A.



Immune response of electroeluted detergent soluble 29 kDa antigen from Setaria digitata (Von Linstow).  


Filariasis is one of the typical parasitic infections which cause immune suppression during the course of infection in both humans and experimental animals. A 29 kDa protein isolated from detergent soluble antigen of S. digitata showed maximum inhibition of cell mediated immune response. The heat inactivated 29 kDa protein was found to be devoid of property of suppression of immune response in the host. Histological study of spleen of BALB/C mice immunized with 29 kDa protein showed changes in regions of spleen such as follicle, trabeculae, capsule, reticuloendothelial cells and eosinophils. The 29 kDa protein, the most reactive of the detergent soluble proteins produced partial suppression of immune response, thereby contributing to the factors responsible for the survival of filarial parasites in hosts. PMID:9854424

John, L; Raj, R K



ARD1, a 64-kDa bifunctional protein containing an 18-kDa GTP-binding ADP-ribosylation factor domain and a 46-kDa GTPase-activating domain.  

PubMed Central

The alpha subunits of the heterotrimeric guanine nucleotide-binding proteins (G proteins) hydrolyze GTP at a rate significantly higher than do most members of the Ras family of approximatelly 20-kDa GTP-binding proteins, which depend on a GTPase-activating protein (GAP) for acceleration of GTP hydrolysis. It has been demonstrated that an inserted domain in the G-protein alpha subunit, not present in the much smaller Ras-like proteins, is responsible for this difference [Markby, D. W., Onrust, R. & Bourne, H. R. (1993) Science 262, 1895-1900]. We report here that ARD1, a 64-kDa protein with an 18-kDa carboxyl-terminal ADP-ribosylation factor (ARF) domain, exhibited significant GTPase activity, whereas the ARF domain, expressed as a recombinant protein in Escherichia coli, did not. Addition of the 46-kDa amino-terminal extension (similarly synthesized in E. coli) to the GTP-binding ARF-domain of ARD1 enhanced GTPase activity and inhibited GDP dissociation. The kinetic properties of mixtures of the ARF and non-ARF domains were similar to those of an intact recombinant ARD1. Physical association of the two proteins was demonstrated directly by gel filtration and by using the immobilized non-ARF domain. Thus, like the alpha subunits of heterotrimeric G proteins, ARD1 appears to consist of two domains that interact to regulate the biological activity of the protein. Images Fig. 1 Fig. 3 Fig. 4

Vitale, N; Moss, J; Vaughan, M



Biogeochemical Indicators of Paleoenvironmental Changes in a Humid Region of Amazonia (Lagoa da Pata, Sao Gabriel da Cachoeira)  

NASA Astrophysics Data System (ADS)

Analysis of four sediment core collected in Lagoa da Pata lake show significant changes in the environmental history of Amazonia during the last 40,000 years. The Lagoa da Pata Lake (0°17'S, 66°40'W) is located on the top of the Morro dos Seis Lagos, an isolated hill in the extensive forested plane surface of high Rio Negro basin. The climate in the region presents mean annual precipitation around 3000 mm without dry season. Three distinct sections are clearly identified in the LPT III, LPT IV and LPT V cores. They consist of upper and lower organic-rich layers, separated by a clastic layer which represents a short period of rapid accumulation around 18 ka BP. Analysis of the organic matter composition by d13C and d15N, total Carbon and Nitrogen, sedimentary chlorophyll concentration and organic matter petrography show three different phases related to organic matter deposition. Between, at least 46,000 and 31,000 14C yr B.P., the lake presented a high water level attested by high accumulation rates of organic carbon. Values of d13C (around -32%) associated to high C/N ratio demonstrate a lignocellulosic material contribution in this phase; the second phase between the 31000 yr 14C B.P. and 18000 14C B.P. is characterised by a lower productivity attested by low sedimentary chlorophyll and total organic carbon values. The d13C values increase with medium values around -28% that may correspond to an input of organic matter rich in C4 plants (grasses). The sedimentation rate decreases significantly. High charcoal fluxes were observed in this period. At around 18000 14C yr BP, there appears to have been a sudden input of clastic material. This is represented by a sandy facies, which exhibits lower carbon contents. From 18000 14C yr B.P. to the present time occurred an increase in lacustrine productivity marked by an increase in carbon and chlorophyll derivate accumulation rates. The C/N values dropped indicting an algae organic matter contribution. It probably corresponds to a lake level rise increasing the production of the system. Increases in charcoal deposition were observed during the upper and medium Holocene. This project is part of the PROFIX/CNPq (processo 54086/01-5) and CNPq (Brazil)-IRD(France) project "PALEOTROPICA" .

Cordeiro, R. C.; Turcq, B.; Mendes, L. G.; Azeredo, J. B.; Moura, M. S.; de Oliveira, A. R.; Monteiro, F.; Santelli, R. E.



Composição florística da vegetação arbórea de um trecho de floresta estacional semidecídua em Viçosa, Minas Gerais, e espécies de maior ocorrência na região  

Microsoft Academic Search

RESUMO - É grande a demanda por estudos ecológicos em florestas que possam embasar trabalhos de recuperação, conservação da biodiversidade e apoio à legislação ambiental. Entre esses estudos, primordialmente está o levantamento da flora. Nesse sentido, este trabalho teve como objetivo determinar a composição florística de um trecho da Reserva da Biologia, em Viçosa, MG, em regeneração natural há 80

Walnir Gomes Ferreira Júnior; Alexandre Francisco da Silva; João Augusto Alves Meira Neto; Carlos Ernesto Gonçalves Reynaud Schaefer; Arildo de Souza Dias; Maíra Ignácio; Maria Cláudia Melo Pacheco de Medeiros



A Novel 90-kDa Stress Protein Induced in Fish Cells by Fish Rhabdovirus Infection  

Microsoft Academic Search

A 90 kDa cellular protein in a fish cell, CHSE-214, showed increased expression by the infection of infectious hematopoietic necrosis virus (IHNV), heat shock, 2-mercaptoethanol, copper sulfate, and cadmium sulfate, and was detected in various kinds of cells such as human, rat, and mouse cells. The molecular mass of the 90 kDa protein was different from those of the hsp90

Wha-Ja Cho; Seung-Ju Cha; Jeong-Wan Do; Jeong-Yun Choi; Joo-Yang Lee; Choon-Soo Jeong; Kyung-Jae Cho; Wan-Sung Choi; Ho-Sung Kang; Han-Do Kim; Jeong-Woo Park



Direct binding of Torpedo syntrophin to dystrophin and the 87 kDa dystrophin homologue  

Microsoft Academic Search

Syntrophin, a 58-kDa membrane-associated protein, is one component of a protein complex associated with dystrophin and other members of the dystrophin family, including the 87-kDa homologue (87K protein). To characterize interactions between syntrophin and 87K protein, we used an in vitro overlay binding assay. We demonstrate that purified Torpedo syntrophin binds directly to dystrophin and 87K. By expressing overlapping regions

Timothy M. Dwyer; Stanley C. Froehner



Determinação de regiões de órbitas diretas estáveis ao redor da Lua  

NASA Astrophysics Data System (ADS)

Neste Trabalho estamos investigando regiões do sistema Terra-Lua que contêm órbitas diretas estáveis ao redor da Lua visando a utilização destas órbitas em futuras missões de veículos espaciais como alternativas de trajetórias com baixo custo de manutenção. Adotando-se o problema restrito de três corpos, Terra-Lua-partícula, a localização e a extensão destas regiões de estabilidade foram investigadas numericamente considerando como estável toda órbita que permanece ao redor a Lua por, no mínimo, 1000 dias com energia de dois corpos da partícula em relação à Lua negativa. A estabilidade de tais regiões está associada a duas famílias de órbitas periódicas simples H1 e H2 e a órbitas quase-periódicas associadas a elas. Uma vez identificadas as regiões de estabilidade passamos a analisá-las introduzindo, paulatinamente, outras interações relevantes ao sistema como as perturbações do Sol (via problema restrito de quatro corpos), das marés, da pressão de radiação e do achatamento terrestre. Os resultados encontrados até o momento mostram que a perturbação do Sol é a única que reduz significativamente o tamanho das regiões de estabilidade. Também estamos investigando o comportamento de tais regiões quando características intrínsecas do sistema como as excentricidades das órbitas da Terra e da Lua e a inclinação da órbita Lua são consideradas. Com este estudo estamos reunindo informações que nos permitirão compreender a evolução das regiões de estabilidade no sistema Terra-Lua, bem como estabelecer parâmetros adequados para a utilização das trajetórias estáveis em futuras missões espaciais. Agradecimentos: Este projeto conta com o apoio do CNPq, da Capes e da Fapesp.

de Melo, C. F.; Winter, O. C.; Vieira Neto, E.



Metal deposits in the Da Hinggan Mountains, NE China: styles, characteristics, and exploration potential  

Microsoft Academic Search

The Da Hinggan Mountains mineral province (DHMP), northeastern China, is divided into three tectonic units and corresponding metallogenic belts. The tectonic units of the Da Hinggan Mountains are the Erguna fold zone on the northwest, the Hercynian fold zone on the north, and the Hercynian fold zone on the south. The corresponding metallogenic belts are the Erguna Cu-Pb-Zn-Ag-Mo-Au belt of

Qingdong Zeng; Jianming Liu; Changming Yu; Jie Ye; Hongtao Liu



Detecção da fase impulsiva de uma explosão solar gigante até 405 GHz  

NASA Astrophysics Data System (ADS)

A explosão ocorrida no dia 25/08/2001 foi uma das mais intensas do presente ciclo solar em ondas de rádio de altas frequências. Foram medidas em ondas milimétricas e submilimétricas, aproximadamente, 105 e vários milhares de unidades de fluxo solar, respectivamente. Apresentamos um estudo deste evento em múltiplas frequências, desde microondas (1GHz), até ondas submilimétricas (405 GHz) detectadas pelo Telescópio Solar para ondas Submilimétricas (SST). Esta base de dados foi complementada utilizando-se o experimento Yohkoh, incluindo a emissão em raios-X duros e raios-g (até 100 MeV), e imagens em raios-X moles da região ativa envolvida. Enfocamos e discutimos principalmente os seguintes aspectos da fase impulsiva do evento: (i) as implicações deduzidas do espectro eletromagnético, obtido pela primeira vez até 405 GHz; (ii) a dinâmica da região ativa. Os resultados mostram que para explicar o espectro rádio observado, são necessários entre 3.5×1037 e 1.5×1039 elétrons acelerados acima de 20 keV em uma região de campo magnético entre 300 e 800 Gauss. A estimativa do fluxo de fótons que seria produzido por estes elétrons, mostra que grande parte deles não precipitou na baixa atmosfera. A evolução temporal da emissão em raios-X moles revela que a configuração magnética da região ativa foi muito dinâmica durante a fase impulsiva da explosão. Em particular, mostramos que a produção dos elétrons altamente energéticos foi iniciada junto com a aparição, na baixa coroa solar, de um novo sistema compacto de estruturas magnéticas. Este fato sugere que os locais de aceleração estão localizados na baixa atmosfera do Sol, como resultado da interação entre o novo sistema compacto e o campo magnético ambiente da região ativa.

Raulin, J.-P.; Makhmutov, V.; Kaufmann, P.; Pacini, A. A.; Luethi, T.; Hudson, H. S.; Gary, D. E.; Yoshimori, M.



The use of Therav?da Buddhist practices and perspectives in mindfulness-based cognitive therapy  

Microsoft Academic Search

This study explores and assesses the nature and practice of Mindfulness-Based Cognitive Therapy (MBCT) from the perspective of Therav?da Buddhism. It is particularly concerned with how both models of training understand and apply ‘mindfulness’. The approach here is, firstly, to examine how the Therav?da understands and employs mindfulness and, secondly, to explore, and more accurately contextualize, the work of MBCT.

Richard Gilpin



A 50-kDa variant form of human surfactant protein D.  


The dominant form of human surfactant protein D (SP-D) is a multimeric collagenous glycoprotein composed of monomeric subunits that have a molecular mass of 43 kDa under reducing conditions. However, in evaluating monoclonal antibodies to human SP-D, an additional monomeric subunit was identified with a reduced molecular mass of 50 kDa. This 50-kDa variant was detected in approximately half of the samples evaluated and was found in lavage fluid from normal subjects, patients with alveolar proteinosis or idiopathic pulmonary fibrosis and in amniotic fluid. This 50-kDa variant had the same amino-terminal sequence, amino acid composition and apparent size of the carboxy-terminal collagenase-resistant fragment (20 kDa) as the 43-kDa subunit. The major difference was in the amino-terminal portion of the molecule and was due to altered glycosylation, as determined by carbohydrate staining, chemical deglycosylation, treatment with N-glycanase and neuraminidase and reduced signals for threonine at positions 5, 9 and 10 during amino-terminal sequencing. After gel filtration chromatography, the 50-kDa form was not present in the high molecular weight fraction, which is commonly used in purification of SP-D, but was found only in the smaller molecular weight fraction of monomers and trimers of SP-D. In conclusion, the 50 kDa-form of surfactant protein D is produced by post-translational glycosylation and does not form higher ordered oligomers, but its precise physiological function remains to be determined. PMID:9864012

Mason, R J; Nielsen, L D; Kuroki, Y; Matsuura, E; Freed, J H; Shannon, J M



Temperature and Gravity Dependence of Trace Element Abundances in Hot DA White Dwarfs (94-EUVE-094)  

NASA Technical Reports Server (NTRS)

EUV spectroscopy has shown that DA white dwarfs hotter than about 45,000 K may contain trace heavy elements, while those hotter than about 50,000 K almost always have significant abundances of trace heavy elements. One of our continuing challenges is to identify and determine the abundances of these trace constituents, and then to relate the observed abundance patterns to the present conditions and previous evolutionary histories of the hot DA white dwarfs.

Finley, David S.



Multiple vital functions of the daughterless (da) gene in Drosophila melanogaster and factors influencing its expression.  


Multiple sub-vital effects, here designated as reductions in fitness S1-S5, for the second chromosome gene 'daughterless' (da) of Drosophila melanogaster were described as (S1) a recessive maternal lethality for daughters, (S2) a reduced fertility of da/da females, (S3) a recessive sub-vital zygotic effect, (S4) a recessive female-specific zygotic effect and (S5) a recessive maternal interaction effect on sons. (For S1-3 see also: Bell, 1954; Sandler, 1972; Cline, 1976). These five distinct effects were initially quantified from estimates of viability in single generation crossing experiments. Dynamic estimates of these fitness parameters were obtained by fitting the elimination rate of da from a series of large random mating cage populations to a recurrence equation by the method of minimum chi-square. The stability of these estimates discerns those effects which are truly pleiotropic versus those due to linked genes. The dynamic estimates supported only S1 and S4 effects. Evidence for S2 and S5 was indeterminate, but the S3 effect was rejected (P less than 0.01). The observed reduction in fitness, supposedly due to this recessive zygotic effect for da, was most likely the result of linked deleterious genes. These results indicate that pleiotropic vital effects observed in single generation test-cross matings may be caused by linked genes rather than the specific mutant per se. This problem is of particular importance when the mutant allele has been maintained with a balancer chromosome. Experiments on the rescue of daughters from da/da mothers with low temperatures during embryogenesis and with dechorionation of eggs were described in which the findings failed to confirm previously reported actions of the da gene. Modifying genes rather than environmental variables were cited as the probable cause for these conflicting results. PMID:3146524

Muir, W M; Bell, A E



Immunoreactivity of a 10-kDa antigen of Mycobacterium tuberculosis.  


Identification of Ag of Mycobacterium tuberculosis recognized by T cells is essential to understanding the pathogenesis of tuberculosis and mechanism(s) of resistance to infection. Previous studies evaluating the immunoreactivity of nitrocellulose transfers of M. tuberculosis Ag separated by SDS-PAGE indicated that a high proportion of M. tuberculosis-reactive T cell lines proliferate in response to a 10-kDa Ag. We therefore purified this Ag from M. tuberculosis culture filtrates and evaluated its immunoreactivity in patients with tuberculous infection. Proliferative responses of PBMC to the 10-kDa Ag were similar to those induced by whole M. tuberculosis and greater than those elicited by other proteins isolated from culture filtrate. Furthermore, in patients with tuberculous pleuritis, proliferative responses to the 10-kDa Ag were higher in pleural fluid mononuclear cells than in PBMC, indicating that T cell reactivity to this Ag is enhanced at the site of disease. The first 15 amino acids of the 10-kDa Ag were identical to those defined previously for Bacillus Calmette-Guérin-a (BCG-a), and a T cell clone recognized the 10-kDa Ag and a peptide of BCG-a, indicating that the 10-kDa Ag corresponds to BCG-a. This Ag elicited IFN-gamma production by pleural fluid mononuclear cells and by PBMC from healthy tuberculin reactors, suggesting that the 10-kDa Ag can enhance macrophage activation and resistance to mycobacterial infection. Our findings indicate that the 10-kDa Ag of M. tuberculosis is highly immunoreactive and should be evaluated for its capacity to elicit protective immunity. PMID:1371791

Barnes, P F; Mehra, V; Rivoire, B; Fong, S J; Brennan, P J; Voegtline, M S; Minden, P; Houghten, R A; Bloom, B R; Modlin, R L




Microsoft Academic Search

RESUMO: O trabalho tem como principal objetivo estimar as constantes elásticas da madeira, empre- gando-se uma metodologia não destrutiva, de ondas ultra-sonoras (ultra-som). Foram utilizadas madei- ras de quatro espécies: Fava Amargosa (Vataireopsis speciosa), Jequitiba Rosa (Cariniana micran- tha), Peroba d'Água (Rauwolfia paraensis) e Peroba Mico (Aspidosperma macrocarpum). Por meio da determinação das velocidades de propagação das ondas ultra-sonoras, baseando-se

Joaquim Carlos Gonçalez; Ailton Teixeira


In vitro degradation of the 32kDa PS II reaction centre protein  

SciTech Connect

The 32kDa thylakoid membrane protein is an integral component of the PS II reaction centre. The protein, although stable in the dark, undergoes light dependent turnover. Light from the UV, visible and far-red spectral regions induce 32kDa protein degradation. To better understand 32kDa protein metabolism, an in vitro degradation system is being developed. It consists of isolated thylakoid membranes than contain radiolabelled protein. The 32kDa protein is actively and specifically degraded when the thylakoid preparation is exposed to UV or visible radiation. The protein is stable in the dark. The herbicides (atrazine and DCMU) inhibit degradation in the in vitro system as they do in vivo. Additionally, several methods of isolating thylakoids are being compared to optimize the 32kDa protein degradation reaction. The preparations will be evaluated based on their ability to permit light dependent degradation of the 32kDa protein without affecting the other membrane components.

Eckenswiller, L.C.; Greenberg, B.M. (Univ. of Waterloo, Ontario (Canada))



OpenDA: Open Source Generic Data Assimilation Environment and its Application in Geophysical Process Models  

NASA Astrophysics Data System (ADS)

Data Assimilation techniques are essential elements in state-of-the-art development of models and their use in operational forecasting and real-time control in the fields of groundwater, surface water and soil systems. In meteorological and atmospheric sciences, steady improvements in numerical weather forecasting and climate prediction over the last couple of decades have been enabled to a large degree by the development of community-based models and data assimilation systems. The hydrologic community should learn from the experiences of the meteorological and atmospheric communities by accelerating the transition of hydrologic DA research into operations and developing community-supported, open-source modeling and forecasting systems and data assimilation tools. In 2010, a community based open source initiative named OpenDA was started. The openDA initiative bears similarities with the well-known openMI initiative. OpenDA makes use of a set of interfaces that describe the interaction between models, observations and data assimilation algorithms. It focuses on flexible applications in portable systems for modeling geophysical processes. It provides a generic interfacing protocol that allows combination of the implemented data assimilation techniques with, in principle, any time-stepping model describing a process (atmospheric processes, 3D circulation, 2D water flow, rainfall-runoff, unsaturated flow, groundwater flow, etc.). Presently, openDA features filtering techniques and calibration techniques. The presentation will give an overview of openDA and the results of some of its practical applications.

Weerts, A.; van Velzen, N.; Verlaan, M.; Sumihar, J.; Hummel, S.; El Serafy, G.; Dhondia, J.; Gerritsen, H.; Vermeer-Ooms, S.; Loots, E.; Markus, A.; Kockx, A.



A novel 550-kDa protein in skeletal muscle of chick embryo: purification and localization.  


We have found a novel protein with a molecular mass of 550 kDa on SDS-polyacrylamide gels, which is abundant in skeletal muscle tissues at an early stage of chick embryonic development. The 550-kDa protein decreased with the progress of development, and only a slight amount of the protein was present in adult chicken skeletal muscle. The 550-kDa protein was purified from the cytoplasm of 18 day embryos by a procedure including ultracentrifugation and gel filtration. The purified 550-kDa protein was essentially free of contaminants as judged by SDS-PAGE. By immunofluorescence and immunoelectron microscopy using the antibody raised against the 550-kDa protein, this protein was shown to be localized in the peripheries of adult muscle fibers and at the Z-disks of isolated myofibrils. These findings have led us to conclude that the 550-kDa protein is a novel myofibrillar protein in chicken skeletal muscle. PMID:7492577

Hattori, A; Wakamatsu, J; Ishii, T; Kuwahara, K; Tatsumi, R



The sandfly fauna (Diptera: Psychodidae: Phlebotominae) of the Parque Estadual da Serra da Tiririca, Rio de Janeiro, Brazil  

PubMed Central

Cutaneous leishmaniasis (CL) in the state of Rio de Janeiro is sporadic and can be characterised as a peridomestic transmission that occurs in modified natural environments. The aim of this work was to study the fauna and ecological characteristics of sandflies in an environmentally protected area (the State Park of Serra da Tiririca) within the remnants of the Atlantic Forest in the municipalities of Niterói and Maricá and their possible relationship with leishmaniasis. Captures were performed using light traps during the night once a month for one year in both sylvatic environments and areas surrounding homes near the park. A total of 1,037 sandflies were captured, belonging to nine genera and 12 species: Evandromyia tupynambai (34.1%), Migonemyia migonei (20.6%), Brumptomyia cunhai (13.8%), Micropygomyia schreiberi (9.7%), Psathyromyia lanei (6.5%), Brumptomyia nitzulescui (5.7%), Evandromyia edwardsi (5.4%), Nyssomyia intermedia (2.8%), Evandromyia cortelezzii (0.6%), Pintomyia bianchigalatiae (0.5%), Lutzomyia longipalpis (0.2%) and Sciopemyia microps (0.1%). Both Mg. migonei and Ny. intermedia may be acting as vectors of CL in this area.

Rodrigues, Andressa Alencastre Fuzari; Barbosa, Vanessa de Araujo; Andrade, Jose Dilermando; Brazil, Reginaldo Pecanha



D-amphetamine (A)-induced dopamine (DA) release is not strictly dependent on newly-synthesized transmitter  

SciTech Connect

A is thought to exert its stimulant effects by releasing DA from a newly synthesized transmitter pool. This hypothesis was evaluated directly by measuring the basal efflux and electrically-evoked release of endogenous DA and dihydroxyphenylacetic acid (DOPAC). In striatal slices from reserpine-treated rabbits A increased DA efflux, reduced DOPAC efflux, and inhibited electrically-evoked /sup 3/H-ACh release in a concentration-dependent manner. These effects could not be mimicked by inhibition of neuronal uptake or MAO, but were blocked by inhibition of DA synthesis or neuronal uptake, and were potentiated by inhibition of MAO. In slices with intact vesicular transmitter stores A induced DA efflux was 2-fold greater than that seen in slices having no vesicular stores. Inhibition of DA synthesis reduced A-induced DA efflux by 60%, but had little effect on the ability of A to inhibit /sup 3/H-ACh release. A also increased the electrical stimulation-evoked overflow of DA (an effect which was attenuated slightly by synthesis inhibition), and potently inhibited DOPAC overflow. These results suggest that: 1) A facilitates efflux of axoplasmic DA by an accelerated exchange diffusion mechanism. The releasable axoplasmic pool is derived from newly synthesized and vesicular transmitter pools; 2) postsynaptic indices of transmitter release may be misleading; and 3) A increases electrically-evoked DA release possibly by inhibiting neuronal uptake.

Parker, E.; Cubeddu, L.



Role of Fluosol-DA 20% in prehospital resuscitation.  


Fluosol-DA 20% (FDA-20) resuscitation has been proposed for prehospital therapy of hemorrhagic shock (HS). Acute HS (mean arterial pressure 60 mm Hg X 90 min, then 40 mm Hg X 30 min) in 24 splenectomized dogs was treated with 50 ml/kg of lactated Ringer's solution (RL) plus a volume equal to 105% of shed blood volume of FDA-20 (group 1), FDA-20 carrier Annex solution (group 2), or RL (group 3). Cardiopulmonary, hemopoietic, hemodynamic, and organ function parameters were measured preshock, at the end of shock, and at 30 and 60 min, and 24 h after resuscitation. FDA-20 produced effective volume expansion, oxygen delivery, and oxygen consumption. The FDA-20 appeared to affect organ function and cells adversely as reflected by a fall in red cell mass and platelet levels and by a rise in liver enzymes, BUN, and serum creatinine. Both the FDA-20 and Annex solution led to a reduction in serum proteins including serum albumin, serum globulin, immunoglobulin-G, and fibrinogen. These reductions are probably due to an oncotically driven factor which appears to maintain an optimal lymph to plasma oncotic ratio. The hydroxyethyl starch in the FDA-20 and the Annex solution, thus, would drive the plasma proteins into the interstitial space. The prolonged prothrombin times and the activated partial thromboplastin times after FDA-20 may be due, in part, to a reduction in the coagulation proteins, although these were not measured. Pending further studies designed to assess the effects of FDA-20 on possible cellular and multiple organ toxicity, clinical studies are premature and unwarranted. PMID:2914450

Elliott, L A; Ledgerwood, A M; Lucas, C E; McCoy, L E; McGonigal, M; Sullivan, M W



ALTERAÇÕES DA FASE ORAL DA DEGLUTIÇÃO EM CRIANÇAS COM PARALISIA CEREBRAL Changes in the oral phase of swallowing in children with cerebral palsy  

Microsoft Academic Search

RESUMO Objetivos: Verificar as alterações na fase oral da deglutição em crianças com paralisia cerebral. Método: Foi conduzido um estudo quantitativo, observacional e transversal, desenvolvido no período de junho a agosto de 2006 no NAMI - Núcleo de Atenção Médica Integrada, em Fortaleza-CE. A amostra constituiu-se de 20 crianças na faixa etária de 1 ano e 6 meses a 8

Karise Santos Vasconcelos



The 37-kDa/67-kDa laminin receptor acts as the cell-surface receptor for the cellular prion protein  

PubMed Central

Recently, we identified the 37-kDa laminin receptor precursor (LRP) as an interactor for the prion protein (PrP). Here, we show the presence of the 37-kDa LRP and its mature 67-kDa form termed high-affinity laminin receptor (LR) in plasma membrane fractions of N2a cells, whereas only the 37-kDa LRP was detected in baby hamster kidney (BHK) cells. PrP co-localizes with LRP/LR on the surface of N2a cells and Semliki Forest virus (SFV) RNA transfected BHK cells. Cell-binding assays reveal the LRP/LR-dependent binding of cellular PrP by neuronal and non-neuronal cells. Hyperexpression of LRP on the surface of BHK cells results in the binding of exogenous PrP. Cell binding is similar in PrP+/+ and PrP0/0 primary neurons, demonstrating that PrP does not act as a co-receptor of LRP/LR. LRP/LR-dependent internalization of PrP is blocked at 4°C. Secretion of an LRP mutant lacking the transmembrane domain (aa 86–101) from BHK cells abolishes PrP binding and internalization. Our results show that LRP/LR acts as the receptor for cellular PrP on the surface of mammalian cells.

Gauczynski, Sabine; Peyrin, Jean-Michel; Haik, Stephane; Leucht, Christoph; Hundt, Christoph; Rieger, Roman; Krasemann, Susanne; Deslys, Jean-Philippe; Dormont, Dominique; Lasmezas, Corinne Ida; Weiss, Stefan



Comparison of PLS1DA, PLS2DA and SIMCA for classification by origin of crude petroleum oils by MIR and virgin olive oils by NIR for different spectral regions  

Microsoft Academic Search

This study compares results obtained with several chemometric methods: SIMCA, PLS2-DA, PLS2-DA with SIMCA, and PLS1-DA in two infrared spectroscopic applications. The results were optimized by selecting spectral ranges containing discriminant information. In the first application, mid-infrared spectra of crude petroleum oils were classified according to their geographical origins. In the second application, near-infrared spectra of French virgin olive oils

O. Galtier; O. Abbas; Y. Le Dréau; C. Rebufa; J. Kister; J. Artaud; N. Dupuy



NADH:ubiquinone oxidoreductase from bovine heart mitochondria. cDNA sequences of the import precursors of the nuclear-encoded 39 kDa and 42 kDa subunits.  

PubMed Central

The 39 kDa and 42 kDa subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria are nuclear-coded components of the hydrophobic protein fraction of the enzyme. Their amino acid sequences have been deduced from the sequences of overlapping cDNA clones. These clones were amplified from total bovine heart cDNA by means of the polymerase chain reaction, with the use of complex mixtures of oligonucleotide primers based upon fragments of protein sequence determined at the N-terminals of the proteins and at internal sites. The protein sequences of the 39 kDa and 42 kDa subunits are 345 and 320 amino acid residues long respectively, and their calculated molecular masses are 39,115 Da and 36,693 Da. Both proteins are predominantly hydrophilic, but each contains one or two hydrophobic segments that could possibly be folded into transmembrane alpha-helices. The bovine 39 kDa protein sequence is related to that of a 40 kDa subunit from complex I from Neurospora crassa mitochondria; otherwise, it is not related significantly to any known sequence, including redox proteins and two polypeptides involved in import of proteins into mitochondria, known as the mitochondrial processing peptidase and the processing-enhancing protein. Therefore the functions of the 39 kDa and 42 kDa subunits of complex I are unknown. The mitochondrial gene product, ND4, a hydrophobic component of complex I with an apparent molecular mass of about 39 kDa, has been identified in preparations of the enzyme. This subunit stains faintly with Coomassie Blue dye, and in many gel systems it is not resolved from the nuclearcoded 36 kDa subunit. Images Fig. 1.

Fearnley, I M; Finel, M; Skehel, J M; Walker, J E



Nucleus-Localized 21.5-kDa Myelin Basic Protein Promotes Oligodendrocyte Proliferation and Enhances Neurite Outgrowth in Coculture, Unlike The Plasma Membrane-Associated 18.5-kDa Isoform  

PubMed Central

The classic myelin basic protein (MBP) family of central nervous system (CNS) myelin arises from transcription start site 3 of the Golli (gene of oligodendrocyte lineage) complex and comprises splice isoforms ranging in nominal molecular mass from 14 kDa to (full-length) 21.5 kDa. We have determined here a number of distinct functional differences between the major 18.5-kDa and minor 21.5-kDa isoforms of classic MBP with respect to oligodendrocyte (OLG) proliferation. We have found that, in contrast to 18.5-kDa MBP, 21.5-kDa MBP increases proliferation of early developmental immortalized N19-OLGs by elevating the levels of phosphorylated ERK1/2 and Akt1 kinases and of ribosomal protein S6. Coculture of N2a neuronal cells with N19-OLGs transfected with the 21.5-kDa isoform (or conditioned medium from), but not the 18.5-kDa isoform, caused the N2a cells to have increased neurite outgrowth and process branching complexity. These roles were dependent on subcellular localization of 21.5-kDa MBP to the nucleus and on the exon II-encoded segment, suggesting that the nuclear localization of early minor isoforms of MBP may play a crucial role in regulating and/or initiating myelin and neuronal development in the mammalian CNS.

Smith, Graham S.T.; Samborska, Bozena; Hawley, Steven P.; Klaiman, Jordan M.; Gillis, Todd E.; Jones, Nina; Boggs, Joan M.; Harauz, George



An Introduction to the DA-T Gibbs Sampler for the Two-Parameter Logistic (2PL) Model and beyond  

ERIC Educational Resources Information Center

The DA-T Gibbs sampler is proposed by Maris and Maris (2002) as a Bayesian estimation method for a wide variety of "Item Response Theory (IRT) models". The present paper provides an expository account of the DA-T Gibbs sampler for the 2PL model. However, the scope is not limited to the 2PL model. It is demonstrated how the DA-T Gibbs sampler for…

Maris, Gunter; Bechger, Timo M.



Percepção Astronômica de Alunos do Ensino Médio da Rede Estadual de São Paulo  

NASA Astrophysics Data System (ADS)

Embora a astronomia seja uma das ciências mais antigas da humanidade e muitos dos conceitos astronômicos serem populares, principalmente nesta época de alta globalização do conhecimento por intermédio de eficientes meios de comunicação e de obtenção da informação, notadamente através da internet, observa-se que uma parcela significativa dos estudantes encontra-se à margem dessas informações. O presente trabalho visa analisar o nível de conhecimento básico dos alunos de Ensino Médio da rede estadual da cidade de Suzano quanto aos fenômenos astronômicos que os rodeiam, tais como a sucessão dos dias e das estações do ano, além de questioná-los sobre fatos genéricos tais como: quais são os astros que se encontram mais próximos do planeta Terra, o que vem a ser o Sol, o Big Bang, um ano-luz, uma estrela cadente, a estrela de Bélem e o que ocasionou a extinção dos dinossauros. Para tanto foi elaborado um formulário constando de questões de múltipla escolha, o qual foi aplicado no primeiro colegial noturno da Escola Estadual Batista Renzi. Num espaço amostral de 34 alunos constatou-se que apenas 29,4% compreendiam a sucessão dos dias da semana, que apenas 20,6% explicaram corretamente as estações do ano, que apenas 20,6% tinham idéia de quais são os objetos celestes mais próximos da Terra, em contraposição 67,6% sabiam classificar corretamente o Sol como estrela, 55,9% relacionavam o Big Bang à origem do universo, apenas 20,6% identificavam um ano-luz como unidade de distância, 32,4% reconheciam uma estrela cadente como meteoro, 41,2% consideravam a estrela de Belém como um cometa e 50,0% explicaram corretamente a extinção dos dinossauros. A presente análise será expandida para as demais classes de primeiro colegial, não somente do período noturno, mas também do diurno da Escola Estadual Batista Renzi, bem como o formulário será devidamente ampliado. Já nesta primeira fase nota-se claramente o pequeno discernimento de eventos astronômicos dos alunos e principalmente a grande confusão sobre o significado correto de termos astronômicos populares.

de Oliveira, E. F.; Voelzke, M. R.; Amaral, L. H.



A constitutively expressed 36 kDa exochitinase from Bacillus thuringiensis HD-1.  


A 36 kDa chitinase was purified by ion exchange and gel filtration chromatography from the culture supernatant of Bacillus thuringiensis HD-1. The chitinase production was independent of the presence of chitin in the growth medium and was produced even in the presence of glucose. The purified chitinase was active at acidic pH, had an optimal activity at pH 6.5, and showed maximum activity at 65 degrees C. Of the various substrates, the enzyme catalyzed the hydrolysis of the disaccharide 4-MU(GlnAc)(2) most efficiently and was therefore classified as an exochitinase. The sequence of the tryptic peptides showed extensive homology with Bacillus cereus 36 kDa exochitinase. The 1083 bp open reading frame encoding 36 kDa chitinase was amplified with primers based on the gene sequence of B. cereus 36 kDa exochitinase. The deduced amino-acid sequence showed that the protein contained an N-terminal signal peptide and consisted of a single catalytic domain. The two conserved signature sequences characteristic of family 18 chitinases were mapped at positions 105-109 and 138-145 of Chi36. The recombinant chitinase was expressed in a catalytically active form in Escherichia coli in the vector pQE-32. The expressed 36 kDa chitinase potentiated the insecticidal effect of the vegetative insecticidal protein (Vip) when used against neonate larvae of Spodoptera litura. PMID:12893268

Arora, Naresh; Ahmad, Tarannum; Rajagopal, R; Bhatnagar, Raj K



mUbiSiDa: A Comprehensive Database for Protein Ubiquitination Sites in Mammals  

PubMed Central

Motivation Protein ubiquitination is one of the important post-translational modifications by attaching ubiquitin to specific lysine (K) residues in target proteins, and plays important regulatory roles in many cell processes. Recent studies indicated that abnormal protein ubiquitination have been implicated in many diseases by degradation of many key regulatory proteins including tumor suppressor, oncoprotein, and cell cycle regulator. The detailed information of protein ubiquitination sites is useful for scientists to investigate the mechanism of many cell activities and related diseases. Results In this study we established mUbiSida for mammalian Ubiquitination Site Database, which provides a scientific community with a comprehensive, freely and high-quality accessible resource of mammalian protein ubiquitination sites. In mUbiSida, we deposited about 35,494 experimentally validated ubiquitinated proteins with 110,976 ubiquitination sites from five species. The mUbiSiDa can also provide blast function to predict novel protein ubiquitination sites in other species by blast the query sequence in the deposit sequences in mUbiSiDa. The mUbiSiDa was designed to be a widely used tool for biologists and biomedical researchers with a user-friendly interface, and facilitate the further research of protein ubiquitination, biological networks and functional proteomics. The mUbiSiDa database is freely available at

He, Bing; Yu, Haiyan; Guo, Xuejiang; Song, Xiaofeng; Sha, Jiahao



Audio watermarking robust against D/A and A/D conversions  

NASA Astrophysics Data System (ADS)

Digital audio watermarking robust against digital-to-analog (D/A) and analog-to-digital (A/D) conversions is an important issue. In a number of watermark application scenarios, D/A and A/D conversions are involved. In this article, we first investigate the degradation due to DA/AD conversions via sound cards, which can be decomposed into volume change, additional noise, and time-scale modification (TSM). Then, we propose a solution for DA/AD conversions by considering the effect of the volume change, additional noise and TSM. For the volume change, we introduce relation-based watermarking method by modifying groups of the energy relation of three adjacent DWT coefficient sections. For the additional noise, we pick up the lowest-frequency coefficients for watermarking. For the TSM, the synchronization technique (with synchronization codes and an interpolation processing operation) is exploited. Simulation tests show the proposed audio watermarking algorithm provides a satisfactory performance to DA/AD conversions and those common audio processing manipulations.

Xiang, Shijun



Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes  

PubMed Central

This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species.

Park, Young-Jin; Park, Jeong-Hyun; Chai, Jong-Yil



Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes.  


This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species. PMID:24850966

Han, Eun-Taek; Yang, Hyun-Jong; Park, Young-Jin; Park, Jeong-Hyun; Chai, Jong-Yil



Effects of ?-Da1a a peptidic ?1A-adrenoceptor antagonist in human isolated prostatic adenoma and anaesthetized rats  

PubMed Central

Background and Purpose ?-Da1a, a 65 amino-acid peptide, has subnanomolar affinity and high selectivity for the human ?1A-adrenoceptor subtype. The purpose of this study was to characterize the pharmacological effects of ?-Da1a on prostatic function, both in vivo and in vitro. Experimental Approach ?-Da1a was tested as an antagonist of adrenaline-induced effects on COS cells transfected with the human ?1A-adrenoceptor as well as on human isolated prostatic adenoma obtained from patients suffering from benign prostatic hyperplasia. Moreover, we compared the effects of ?-Da1a and tamsulosin on phenylephrine (PHE)-induced increases in intra-urethral (IUP) and arterial pressures (AP) in anaesthetized rats, following i.v. or p.o. administration. Key Results On COS cells expressing human ?1A-adrenoceptors and on human prostatic strips, ?-Da1a inhibited adrenaline- and noradrenaline-induced effects. In anaesthetized rats, ?-Da1a and tamsulosin administered i.v. 30 min before PHE significantly antagonized the effects of PHE on IUP. The pKB values for tamsulosin and ?-Da1a for this effect were similar. With regards to AP, ?-Da1a only reduced the effect of PHE on AP at the lowest dose tested (10 ?g·kg?1), whereas tamsulosin significantly reduced PHE effects at doses between 10 and 150 ?g·kg?1. Conclusions and Implications ?-Da1a exhibited a relevant effect on IUP and a small effect on AP. In contrast, tamsulosin antagonized the effects of PHE on both IUP and AP. We conclude that ?-Da1a is more uroselective than tamsulosin. ?-Da1a is the most selective peptidic antagonist for ?1A-adenoceptors identified to date and could be a new treatment for various urological diseases.

Palea, S; Maiga, A; Guilloteau, V; Rekik, M; Guerard, M; Rouget, C; Rischmann, P; Botto, H; Camparo, P; Lluel, P; Gilles, N



Identification of an abundant 56 kDa protein implicated in food allergy as granule-bound starch synthase.  


Rice, the staple food of south and east Asian counties, is considered to be hypoallergenic. However, several clinical studies have documented rice-induced allergy in sensitive patients. Rice proteins with molecular weights of 14-16, 26, 33, and 56 kDa have been identified as allergens. Recently, it was documented that the 56 kDa rice allergen was responsible for rice-induced anaphylaxis. The 14-16 kDa allergens have been identified as ?-amylase inhibitors; the 26 kDa protein has been identified as ?-globulin; and the 33 kDa protein has been identified as glyoxalase I. However, the identity of the 56 kDa rice allergen has not yet been determined. In this study, we demonstrate that serum from patients allergic to maize shows IgE binding to a 56 kDa protein that was present in both maize and rice but not in the oil seeds soybean and peanut. The 56 kDa IgE-binding protein was abundant in the rice endosperm. We have purified this protein from rice endosperm and demonstrated its reactivity to IgE antibodies from the serum of maize-allergic patients. The purified protein was subjected to matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry analysis, resulting in identification of this rice allergen as granule-bound starch synthase, a product of the Waxy gene. Immunoblot analysis using protein extracts from a waxy mutant of rice revealed the absence of the 56 kDa IgE-binding protein. Our results demonstrate that the 56 kDa rice allergen is granule-bound starch synthase and raise the possibility of using waxy mutants of rice as a potential source of the hypoallergenic diet for patients sensitized to the 56 kDa rice allergen. PMID:23675783

Krishnan, Hari B; Chen, Ming-Hsuan




Microsoft Academic Search

A busca por um ensino de física que desperte o senso crítico de alunos e professore s sobre assuntos relacionados à C iência tem promovido a criação de diversas propostas e iniciativas que envolvam a discussão da Natureza da Ciência (NdC). A NdC pode ser definida como um conjunto de saberes sobre a C iência e sobre os processos que

Breno Arsioli Moura; Cibelle Celestino Silva



Solution Structure of the 128 kDa Enzyme I Dimer from Escherichia coli and Its 146 kDa Complex with HPr Using Residual Dipolar Couplings and Small and Wide-Angle X-ray Scattering  

Microsoft Academic Search

The solution structures of free Enzyme I (EI, 128 kDa, 575 x 2 residues), the first enzyme in the bacterial phosphotransferase system, and its complex with HPr (146 kDa) have been solved using novel methodology that makes use of prior structural knowledge (namely, the structures of the dimeric EIC domain and the isolated EIN domain both free and complexed to

Charles D. Schwieters; Jeong-Yong Suh; Alexander Grishaev; Rodolfo Ghirlando; Yuki Takayama; G. Marius Clore



Spectroscopy of the DA white dwarfs - Automatic atmospheric parameterization and mass distribution  

NASA Technical Reports Server (NTRS)

A method for the automatic calculation of the atmospheric parameters (Teff and log g) of hydrogen-rich degenerate stars from low-resolution spectra is described, and then applied to the spectra of 53 DA white dwarfs. A value for the width of the DA mass distribution of sigma M/solar-M not greater than +0.10 is obtained using the proposed approach. The data indicate that the distribution is asymmetrically skewed to low masses; however, there is also evidence of a high-mass non-Gaussian tail.

Mcmahan, Robert K.



DAEdALUS: A New Search for CP Violation in the Neutrino Sector  

NASA Astrophysics Data System (ADS)

The DAEdALUS experiment offers a novel approach to measuring CP violation in the neutrino sector. The design uses multiple cyclotrons to produce intense beams of neutrinos from the decay-at-rest of pions and muons. Short-baseline muon- to electron-antineutrino oscillations are observed via inverse beta decay in an ultra-large water Cerenkov detector at DUSEL. The experiment's high-statistics, low-background output yields a CP violation sensitivity competitive with that of LBNE. Together, the complementary designs of DAEdALUS and LBNE provide the highest sensitivity of any presently proposed CP violation search.

Carr, Rachel



Intervenções em consciência fonológica e aprendizagem da linguagem escrita 1 Interventions in phonological conscience and learning of the written language  

Microsoft Academic Search

Resumo: Vários estudos evidenciam alta associação entre a consciência fonológica e as habilidades de ler e escrever. Este trabalho tem como objetivo identificar e analisar pesquisas de intervenção em consciência fonológica e seus efeitos sobre a aprendizagem da linguagem escrita. São identificados três tipos de pesquisas: com adultos analfabetos, com crianças em dificuldade na aprendizagem da escrita e com pré-escolares.

Maria José dos Santos


Modello di valutazione dell'attitudine fisica del territorio per la realizzazione di impianti cedui da biomassa in Italia  

Microsoft Academic Search

Le piantagioni governate a ceduo a turno breve (short rotation forestry - SRF) rappresentano il modello di arboricoltura da legno maggiormente idoneo alla produzione di biomassa lignocellulosica da destinare a usi energetici. La limitata disponibilità di risorse e la rete sempre più complessa di relazioni che coinvolgono l'uso del territorio pon- gono l'esigenza di una attenta programmazione territoriale di questo

Riccardo Salvati; Gherardo Chirici; Piermaria Corona



Avaliação das normas de biossegurança nas clínicas odonto- lógicas da UFPE Evaluation of biosecutrity norms in UFPE dental clinics  

Microsoft Academic Search

Key-words O objetivo deste trabalho foi observar o cumprimento das normas de biossegurança e os cuidados com os riscos ocupacionais pelos alunos que atenderam nas clínicas de Odontologia Preventiva, Odontopediatria, Dentística, Endodontia e nos procedimentos cirúrgicos nas clínicas de Periodontia e Cirurgia, da Faculdade de Odontologia da Universidade Federal de Pernambuco. Este trabalho consistiu em um estudo observacional, no qual

Márcia Maria; Vendiciano Barbosa Vasconcelos; Catarina da Mot; Vasconcelos Brasi; Cláudia Cristina; Brainer de Oliveira Mot; Rabelo de Carvalho


Induction of fibroblast 92 kDa gelatinase/type IV collagenase expression by direct contact with metastatic tumor cells.  


Previous studies have correlated release of the 92 kDa type IV collagenase/gelatinase by tumor cells in culture with metastatic potential. We have now demonstrated that the ability of tumor cells that do not express the 92 kDa gelatinase to induce release of this metalloproteinase from normal fibroblasts may also be associated with the metastatic phenotype. A transformed rat embryo cell line, 2.8, failed to release the 92 kDa gelatinase alone in culture, but gave rise to metastatic tumors whose explants contained the 92 kDa gelatinase. In contrast, a non-metastatic transformed cell line, RA3, did not express the 92 kDa gelatinase alone in culture or in tumor explants. To explore the mechanisms that might govern host-tumor cell interactions in this system, we have studied the effects of co-culture of these transformed cell lines with rat embryo fibroblasts (REF) in culture. 92 kDa gelatinase expression was induced by co-culture of 2.8 with REF, but co-culture of the non-metastatic line RA3 with REF did not result in induction of the 92 kDa gelatinase. The 92 kDa gelatinase in these co-cultures was released by the fibroblasts; methanol-fixed 2.8 cells induced 92 kDa gelatinase expression in REF, but fixed REF cells did not induce enzyme expression in 2.8 cells. This suggested that cell contact was required for induction, which was confirmed by showing that 92 kDa gelatinase induction in co-culture was abolished by separating REF from 2.8 by solute-permissive membranes. In addition, REF could not be stimulated to produce the 92 kDa gelatinase by 2.8-derived conditioned medium, by 2.8-derived extracellular matrix, or by isolated matrix components. These data indicate that metastatic tumor cells can induce 92 kDa gelatinase expression in fibroblasts through a mechanism dependent upon cell contact. In situ hybridization of nude mouse tumors derived from these transformed cell lines revealed 92 kDa gelatinase expression in the stroma of tumors from 2.8, but not in tumors from RA3. Therefore, the experiments based on in vitro co-culture of tumor cells and fibroblasts, together with the in situ localization of mRNA to host cells, suggest that host production of the 92 kDa gelatinase may occur in response to direct contact with metastatic tumor cells. PMID:8207074

Himelstein, B P; Canete-Soler, R; Bernhard, E J; Muschel, R J



A 23-kDa protein as a substrate for protein kinase C in bovine neutrophils. Purification and partial characterization  

SciTech Connect

In {sup 32}P{sub i}-loaded bovine neutrophils stimulated with phorbol myristate acetate (PMA), radioactivity was preferentially incorporated into a protein of low molecular mass, suggesting a PKC-dependent phosphorylation. This protein, termed 23-kDa protein, was predominantly localized in the cytosol. The apparent molecular mass of the purified protein range between 20 and 23 kDa. In the absence of mercaptoethanol, a dimer accumulated. Homogeneity of the 23-kDa protein was verified by 2D-PAGE analysis. Gel isoelectric focusing (IEF) of the purified 23-kDa protein followed by Coomassie blue staining allowed the visualization of our discrete protein bands with isoelectric points ranging between pH 6.3 and 6.7. Phosphorylation of the 23-kDa protein by ({gamma}-{sup 32}P)ATP in the presence of bovine neutrophil PKC supplemented with Ca{sup 2+}, phosphatidylserine, and diacylglycerol or with PMA occurred on serine and required the presence of mercaptoethanol. IEF of the {sup 32}P-labeled 23-kDa protein followed by autoradiography revealed for discrete bands with distinct isoelectric points similar to those of the bands stained by Coomassie blue after IEF on nonlabeled 23-kDa protein. The bands of the 23-kDa protein resolved by IEF and transfered to nitrocellulose showed ability to bind ({sup 35}S)GTP-{gamma}-S. The immunoreactivity of antibodies raised in rabbits against the bovine neutrophil 23-kDa protein was demonstrated on immunoblots after SDS-PAGE. The 23-kDa protein differed also from several other proteins of similar molecular mass that have been identified in neutrophils, namely, calmodulin, the small subunit of the low-potential cytochrome b, and a low molecular weight protein which is ADP-ribosylated by the botulinum toxin.

Stasia, M.J.; Dianoux, A.C.; Vignais, P.V. (Centre d'Etudes Nucleaires (France))



Fermentação da proteína de seis alimentos por microrganismos ruminais, incubados puros ou com monensina ou rumensin®  

Microsoft Academic Search

RESUMO - Avaliaram-se os efeitos da fermentação in vitro de seis alimentos: fubá de milho (FM), farelo de soja (FS), farelo de trigo (FT), sorgo (SO), glúten de milho (GM) e uréia (UR), incubados puros ou com o antibiótico monensina, esse na forma pur a para análise (Monensina) ou comercial (Rumensin®). O experimento constituiu-se de 18 unidades experimentais (três alimentos

Natália Guarino Souza Barbosa; Rogério de Paula Lana; Antônio Bento Mâncio; Arnaldo Chaer Borges; Cesar de Queiroz; Juliana Silva Oliveira



O outro pé da sereia: História[s] na pós-colonialidade  

Microsoft Academic Search

This article discusses Mia Couto's novel O outro pé da sereia (2006) in terms of the novel's significant readings of Mozambican history, tradition and cultural context. Postcolonial theory provides a critical framework for the study of questions that appear in the novel and represent a set of issues that are central to both Mia Couto's work and to the broader

Elena Brugioni



Influência do veículo na eficácia da reposição de potássio em ratos hipocalêmicos  

Microsoft Academic Search

Resumo Introdução: Pacientes após cirurgia cardíaca são comumente tratados com diuréticos para controle de volume plasmático. A preocupação de distúrbios hipocalêmicos em adultos antes, durante ou após a cirurgia já foi ressaltada anteriormente, visto o risco de arritmias cardíacas. Clinicamente, a diluição da solução de potássio (K+) para administração por via intravenosa, em situações que requerem a sua reposição é

Márcio Petenusso; Vitor Engrácia Valenti; Luiz Carlos de Abreu; Eduardo Colombari; Fernando Luiz Affonso Fonseca; Monica Akemi Sato



Migration from can coatings: Part 2. Identification and quantification of migrating cyclic oligoesters below 1000?Da  

Microsoft Academic Search

Metal cans for food use can be coated with lacquers based on polyester resins. Recent research has focussed on the identification and quantification of migrants released by coatings that are potentially absorbable (below 1000?Da). The presented method describes a procedure that was optimized to hydrolyse the polyester migrants into their monomers, polyvalent acids and polyols. The polyols were identified by

A. Schaefer; V. A. Ohm; T. J. Simat



32 CFR 644.512 - DA-SBA joint set-aside determination.  

Code of Federal Regulations, 2013 CFR

...CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand and Stone § 644.512 DA-SBA joint set-aside determination. Section 15 of the Small Business Act (15 U.S.C. 644),...



Identification and isolation of a 155-kDa protein with neuropathy target esterase activity.  


A method is presented for the isolation of a 155-kDa protein that possesses phenyl valerate hydrolysis activity in the presence of paraoxon but is inhibited by mipafox; the functional definition of neuropathy target esterase (neurotoxic esterase; NTE). Microsomes, isolated from 18-day-old chicken embryos were treated with phospholipase A2 to solubilize the NTE activity. The extract was then combined with polyoxyethylene W1 detergent and resolved by gel filtration chromatography to yield an active fraction with an approximate mass of 200 kDa. This fraction was further purified by preparative isoelectric focusing and native electrophoresis to yield two separate bands possessing NTE activity. The slower migrating band was highly enriched in a 155-kDa protein that was identified as a source of the NTE activity by affinity chromatography using 3-(9'-mercaptononylthio)-1,1,1-trifluoro-propan-2-one bound to Sepharose CL6B. This represents the first report of the isolation of NTE in its active form and aids in the confirmation of the 155-kDa protein as the most likely candidate for NTE. PMID:8812209

Mackay, C E; Hammock, B D; Wilson, B W



Seasonal Mosquito Fluctuations in Da Nang, South Vietnam, Based on Three Prevalent Species.  

National Technical Information Service (NTIS)

A light trap survey was conducted in Da Nang, Vietnam for 42 weeks beginning 1 June 1969. Over 150,000 specimens were sorted and identified. The most numerous mosquitoes collected were C. tritaeniorhynchus, A. Sinensis, and A. vagus. C. tritaeniorhynchus ...

T. G. Floore R. H. Grothaus W. F. Miner



A Integracao de Ensino das Ciencias da Saude (An Integrated Medical Education Program [in Brazil]).  

ERIC Educational Resources Information Center

At the Sixth Annual Reunion of the Brazilian Association of Medical Schools (VI Reuniao Anual da Associacao Brasileira de Escolas Medicas) leaders in the Brazilian medical profession proposed an integrated educational program for training students in the fields of medicine and public health. Under Brazil's present system of education, all…

Pourchet-Campos, M. A.; Guimaraes Junior, Paulino


Long-term evolution of 1991 DA: A dynamically evolved extinct Halley-type comet  

NASA Astrophysics Data System (ADS)

The long-term dynamical evolution of 21 variational orbits for the intermediate-period asteroid 1991 DA was followed for up to +/-105 years from the present. 1991 DA is close to the 2:7 resonance with Jupiter; it has avoided close encounters, within 1 AU, with this planet for at least the past 30,000 years, even at the node crossing. The future evolution typically shows no close encounters with Jupiter within at least 50,000 years. This corresponds to the mean time between node crossings with either Jupiter or Saturn. Close encounters with Saturn and Jupiter lead to a chaotic evolution for the whole ensemble, while secular perturbations cause large-amplitude swings in eccentricity and inclination (the latter covering the range 15 deg approximately less than i approximately less than 85 deg) which correlate with deep excursions of the perihelion distance to values much less than 1 AU. These variations are similar to those found in P/Machholz and a variety of other high-inclination orbits, e.g., P/Hartley-IRAS. We emphasize the connection between the orbital evolution of 1991 DA and that of Halley-type comets. If 1991 DA was once a comet, it is not surprising that it is now extinct.

Hahn, Gerhard; Bailey, M. E.



Francis Bacon e as marés: a concepção da natureza e o mecanicismo  

Microsoft Academic Search

Esta breve introdução visa duas tarefas ao mesmo tempo: primeiro, informar sobre a época provável de composição e o lugar ocupado, no conjunto da obra de Francis Bacon (1561-1626), pelo pequeno tratado intitulado De fluxu et refluxu maris (Do fluxo e refluxo do mar); segundo, expor a solução que nele se apresenta para o problema das marés, mostrando tratar-se de

Pablo Rubén Mariconda



Precise Estimates of the Synthetic Spectral Distances to DA White Dwarfs  

Microsoft Academic Search

Using recent photometric calibrations, we develop greatly improved distance estimates for DA white dwarfs using multi-band synthetic photometry based on spectroscopic temperatures and gravities. Very good correlations are shown to exist between our spectroscopically based photometric distance estimates and those derived from trigonometric parallaxes. We investigate the uncertainties involved in our distance estimates, as well as discuss the circumstances where

J. B. Holberg; P. Bergeron; A. Gianninas



Preliminary Design Study of High-Power H2+ Cyclotrons for the DAEdALUS Experiment  

Microsoft Academic Search

This report provides a first design for H2+ accelerators as the DAEdALUS neutrino sources. A description of all aspects of the system, from the ion source to the extracted beam, is provided. The analysis provides a first proof of principle of a full cyclotron system which can provide the necessary beam power for the CP violation search proposed by the

L. Calabretta; L. Celona; S. Gammino; D. Rifuggiato; G. Ciavola; M. Maggiore; L. A. C. Piazza; J. R. Alonso; W. A. Barletta; A. Calanna; J. M. Conrad



Long-term evolution of 1991 DA: A dynamically evolved extinct Halley-type comet  

NASA Technical Reports Server (NTRS)

The long-term dynamical evolution of 21 variational orbits for the intermediate-period asteroid 1991 DA was followed for up to +/-10(exp 5) years from the present. 1991 DA is close to the 2:7 resonance with Jupiter; it has avoided close encounters, within 1 AU, with this planet for at least the past 30,000 years, even at the node crossing. The future evolution typically shows no close encounters with Jupiter within at least 50,000 years. This corresponds to the mean time between node crossings with either Jupiter or Saturn. Close encounters with Saturn and Jupiter lead to a chaotic evolution for the whole ensemble, while secular perturbations cause large-amplitude swings in eccentricity and inclination (the latter covering the range 15 deg approximately less than i approximately less than 85 deg) which correlate with deep excursions of the perihelion distance to values much less than 1 AU. These variations are similar to those found in P/Machholz and a variety of other high-inclination orbits, e.g., P/Hartley-IRAS. We emphasize the connection between the orbital evolution of 1991 DA and that of Halley-type comets. If 1991 DA was once a comet, it is not surprising that it is now extinct.

Hahn, Gerhard; Bailey, M. E.




EPA Science Inventory

Maternal Atrazine (ATR) alters hypothalamic dopamine (HYP-DA) and serum prolactin (sPRL) in male pups. 1Christopher Langdale, 2Tammy Stoker and 2Ralph Cooper. 1 Dept. of Cell Biology, North Carolina State University College of Veterinary Medicine, Raleigh, NC. 2 Endocrinology ...


Brominated and radioiodinated derivatives of methylphenidate (MP): Potential imaging agents for the dopamine (DA) transporter  

SciTech Connect

MP (Ritalin) is a psychomotor stimulant used in the treatment of attention-deficit hyperactivity disorder. The therapeutic properties of MP are thought to be mediated by its binding to a site on the DA transporter, resulting in inhibition of DA reuptake and enhanced levels of synaptic dopamine. MP also inhibits reuptake of norepinephrine (NE) in vitro. MP has two chiral centers, but its pharmacological activity is believed due solely to the d-threo isomer. We have found that d,l-threo-C-11 MP has favorable properties for PET studies, and therefore examined the effects of incorporating halogen atoms into the phenyl ring of MP, with a view to preparing C-11 and I-123 MP analogs as potential PET/SPECT tracers. We synthesized the 2-, 3- and 4-bromo MP analogs from the corresponding bromophenylacetonitriles by modification of the original synthesis of MP. In in vitro binding assays all three d,l-threo bromo compounds had higher affinities than MP for DA transporter sites labeled with tritiated WIN 35,428 (3->4-, 2->MP). They also showed high activity with NE reuptake sites labeled with tritiated nisoxetine. They were active in vivo as demonstrated by inhibition of heart uptake of tritiated NE in the mouse, and elevation of striatal extracellular DA (microdialysis) and stimulation of locomotor activity in the rat.

Pan, D.; Gatley, S.J.; Dewey, S.L. [Brookhaven National Lab., Upton, NY (United States)] [and others



Determinação do extremo de baixa luminosidade da função de luminosidade através de simulações de Monte Carlo  

NASA Astrophysics Data System (ADS)

Neste trabalho apresentamos os principais resultados do estudo da população de galáxias anãs de baixo brilho superficial dos grupos compactos HCG44 e HCG68. A inclinação do extremo de baixa luminosidade a da função de luminosidade desses grupos foi estimado através de simulações de Monte Carlo. O método consistiu em simular galáxias anãs de baixo brilho superficial em imagens reais seguindo uma distribuição de magnitudes dada uma função de Schechter. Foram testados vários modelos com diferentes valores para os parâmetros M* e a da função de Schechter, e para cada um dos modelos foi repetido o mesmo processo de recuperação, seleção e análise usados com as galáxias anãs dos grupos. Os resultados de cada modelo foram então comparados com o observado nos grupos. Nossos resultados indicam que a distribuição de magnitudes (para magnitudes absolutas até MR»-12) observadas nos grupos são compatíveis com uma função de luminosidade de Schechter dada por um M* = -16 e um a entre -1.0 e -1.4, e portanto de acordo com a tendência observada de uma inclinação do extremo de baixa luminosidade da função de luminosidade de grupos mais plana (~1.2, Trentham & Tully 2002, MNRAS, 335, 712) que esperado pelo modelo hierárquico de formação de estruturas CDM (Cold Dark Matter - Matéria Escura Fria).

Campos, P. E.; Mendes de Oliveira, C.



A Utilização da Astronomia como Tema Interdisciplinar e Aplicações de Objetos de Aprendizagem  

NASA Astrophysics Data System (ADS)

Este trabalho visa analisar a possibilidade de relacionar conteúdos aplicados no ensino fundamental e médio de forma interdisciplinar por intermédio da astronomia, com a intervenção de objetos de aprendizagem que possam integrar as disciplinas e a utilização de recursos tecnológicos. Em uma pesquisa prévia com 20 professores de uma escola estadual situada na cidade de Guarulhos foi observado que apenas 25% dos professores utilizam algum recurso tecnológico para o desenvolvimento de conteúdos pertinentes à sua disciplina, tais como sites e softwares educativos, sendo que a maioria absoluta continua ensinando apenas com livros didáticos. A maior parte dos professores apresenta dificuldades em trabalhar sua disciplina de forma interdisciplinar, ou seja, 75% dos professores preferem aplicar os conteúdos seguindo uma hierarquia linear de tópicos, evitando a discussão de temas que de alguma forma estão relacionados. A astronomia pode vir à fascinar o ser humano e despertar sua curiosidade promovendo um maior interesse no aprendizado, podendo favorecer análises interdisciplinares de forma lógica e objetiva, desta forma colocar a astronomia como tema motivador interdisciplinar, pode ser relevante no que se refere ao distanciamento da fragmentação dos conteúdos. No Estado de São Paulo, a implantação da proposta curricular no ensino fundamental e médio mostra claramente a inserção da astronomia na maior parte das séries, principalmente na 6ª série em que todo o bimestre se fala de astronomia.

da Silva, L. A.; Voelzke, M. R.



Determinação da massa de júpiter a partir das órbitas de seus satélites: um experimento didático  

NASA Astrophysics Data System (ADS)

Este trabalho apresenta o roteiro piloto de uma prática observacional em astronomia, junto com os primeiros resultados obtidos nesta fase de implementacão. O projeto, que será executado em duas etapas, visa introduzir noções de Astronomia a alunos do Ensino Médio e iniciantes nos cursos de Física. O experimento consiste em medir as órbitas dos satélites Galileanos e, a partir da análise dos dados coletados, verificar a validade da Lei das órbitas de Kepler, determinando a massa do planeta Júpiter. Em uma primeira etapa, as observações serão feitas utilizando um telescópio Meade LX200 10" e câmera CCD para obter uma seqüência de imagens do planeta, que possibilitará medir o movimento de seus satélites. A segunda etapa terá início a partir do funcionamento do telescópio em modo robótico, com a possibilidade de observações via internet por instituições de ensino. Para o desenvolvimento deste experimento foram inicialmente coletadas várias imagens de Júpiter obtidas com os instrumentos citados acima. Estas imagens serviram como base para confecção dos roteiros para a experiência no nível médio e superior. Os roteiros serão inicialmente apresentados em uma home-page. Nela também se buscará uma contextualização histórica da experiência bem como o estabelecimento de relações com professores e alunos, propostas metodológicas e a disponibilização dos programas computacionais necessários para a utilização "on-line" pelos usuários. O projeto conta com apoio da Fundação VITAE.

Schlickmann, M. S.; Saito, R. K.; Becker, D. A.; Rezende, M. F., Jr.; Cid Fernandes, R.



Kaonic Atoms/nuclei Measurements at DA?NE:. Siddharta and Amadeus  

NASA Astrophysics Data System (ADS)

The DA?NE electron-positron collider at the Laboratori Nazionali di Frascati dell'INFN has made available a unique quality negative kaons “beam”. Two experiments, SIDDHARTA and AMADEUS, will use this beam to perform unprecedented precision measurements on kaonic atoms and kaonic nuclei. The scientific cases, together with the experimental setups, are briefly presented in this paper.

Curceanu (Petrascu), C.; Zmeskal, J.


Kaonic atoms and nuclei search at the DA?NE accelerator  

NASA Astrophysics Data System (ADS)

The DA?NE electron-positron collider at the Laboratori Nazionali di Frascati dell'INFN has made available a unique quality low-energy negative kaons "beam". Two experiments, SIDDHARTA and AMADEUS, will use this kaon source to perform unprecedented precision measurements on kaonic atoms and kaonic nuclei. The scientific cases, together with the experimental setups, are briefly presented in this paper.

Curceanu (Petrascu), C.; Siddharta Collaboration; Amadeus Collaboration



Next Generation PanDA Pilot for ATLAS and Other Experiments  

NASA Astrophysics Data System (ADS)

The Production and Distributed Analysis system (PanDA) has been in use in the ATLAS Experiment since 2005. It uses a sophisticated pilot system to execute submitted jobs on the worker nodes. While originally designed for ATLAS, the PanDA Pilot has recently been refactored to facilitate use outside of ATLAS. Experiments are now handled as plug-ins such that a new PanDA Pilot user only has to implement a set of prototyped methods in the plug-in classes, and provide a script that configures and runs the experiment-specific payload. We will give an overview of the Next Generation PanDA Pilot system and will present major features and recent improvements including live user payload debugging, data access via the Federated XRootD system, stage-out to alternative storage elements, support for the new ATLAS DDM system (Rucio), and an improved integration with glExec, as well as a description of the experiment-specific plug-in classes. The performance of the pilot system in processing LHC data on the OSG, LCG and Nordugrid infrastructures used by ATLAS will also be presented. We will describe plans for future development on the time scale of the next few years.

Nilsson, P.; Barreiro Megino, F.; Caballero Bejar, J.; De, K.; Hover, J.; Love, P.; Maeno, T.; Medrano Llamas, R.; Walker, R.; Wenaus, T.; Atlas Collaboration



Molluscan mega-hemocyanin: an ancient oxygen carrier tuned by a ~550 kDa polypeptide  

Microsoft Academic Search

BACKGROUND: The allosteric respiratory protein hemocyanin occurs in gastropods as tubular di-, tri- and multimers of a 35 × 18 nm, ring-like decamer with a collar complex at one opening. The decamer comprises five subunit dimers. The subunit, a 400 kDa polypeptide, is a concatenation of eight paralogous functional units. Their exact topology within the quaternary structure has recently been

Bernhard Lieb; Wolfgang Gebauer; Christos Gatsogiannis; Frank Depoix; Nadja Hellmann; Myroslaw G Harasewych; Ellen E Strong; Jürgen Markl



As relações da Revista Estudos Feministas com os movimentos de mulheres  

Microsoft Academic Search

R R R R Resumo esumo esumo esumo esumo: Este artigo tem como objetivo refletir sobre o diálogo da REF, nesses últimos 15 anos, com os movimentos de mulheres no Brasil. Recupera os temas dos diversos dossiês que, em grande medida, focaram temáticas de extrema relevância e atualidade para a agenda política do feminismo. Resgata alguns desses dossiês como exemplos

Leila Linhares Barsted



Influência da irrigação e do genótipo na produção de castanha em cajueiro-anão-precoce  

Microsoft Academic Search

(2) Resumo - Avaliou-se a influência da irrigação e do genótipo na produção de castanha em cajueiro-anão- precoce (Anacardium occidentale L.) durante três anos. Foram estudados três clones (CP 09, CP 76 e CP 1001) e quatro regimes hídricos (testemunha sem irrigação e intervalos de irrigação de um, três e cinco dias). O delineamento experimental foi em blocos ao acaso,

Vitor Hugo de Oliveira; Levi de Moura Barros; Raimundo Nonato de Lima



[Release characteristics in vitro and pharmacokinetics of da chuanxiong fang multiunit drug delivery system in rats].  


The drug release characteristics ofDa Chuanxiong Fang multiunit drug delivery system (DCXFMDDS) in vivo and in vitro were evaluated. Ferulic acid (FA) and senkyunolide I (SI) were used as marker components, which were two of the effective components of Da Chuanxiong Fang. And their contents were determined by HPLC. Drug release characteristics in vitro of DCXFMDDS and Da Chuanxiong pills and pharmacokinetics characteristics of DCXFMDDS and Da Chuanxiong Fang active fraction (DCXFAF) in rats were compared. It was obvious that FA released from the DCXFMDDS in a sustained fashion but SI in a fast fashion both in vitro and in vivo. The releasing process and the releasing mechanism of FA and SI from DCXFMDDS were different, but the AUC value indicated that compared with DCXFAF the extent of absorption of FA and SI from DCXFMDDS was increased. Though from the same multiunit drug delivery system, FA an SI had different drug release characteristics both in vitro and in vivo, and that may be one of the reason why DCXFMDDS has the good properties such as rapid and long-lasting effect and high efficiency. PMID:22121790

Wei, Yuan-feng; Zhang, Ning; Lin, Xiao; Feng, Yi



Amplatzer Closure of Atrial Septal Defect and da Vinci Robot-Assisted Repair of Vascular Ring  

Microsoft Academic Search

Technology for minimally invasive approaches to congenital heart disease is a rapidly evolving field. This case report reviews a novel approach to combining two of the newer technologies available to treat a pediatric patient with an atrial septal defect (ASD) and a vascular ring. This report is the first to describe the use of the da Vinci surgical system to

R. G. Ohye; E. J. Devaney; J. Graziano; A. Ludomirsky



UMA EXPERIÊNCIA COM O PROJETO MANHATTAN NO ENSINO FUNDAMENTAL An experience with the Manhattan Project in the Elementary School  

Microsoft Academic Search

Resumo: O presente artigo discute uma dissertação de mestrado que utilizou uma perspectiva educacio- nal voltada à formação de cidadãos e defendida na proposta de Edgar Morin e no relatório da UNESCO sobre educação para o século XXI. Para isso, aborda a importância de uma nova prática escolar, preocupa- da em manter o estudante como centro das atividades em sala

Rafaela Samagaia; Luiz O. Q. Peduzzi



Microsoft Academic Search

A maior parte dos estudos que se debruçam sobre as tecnologias de informação e comunicação (TIC) e as crianças tentam demonstrar as potencialidades e benefícios das primeiras em relação às segundas. A perspectiva inversa - o que as crianças fazem das tecnologias - é muito menos documentada na pesquisa. A partir do quadro teórico de referência da Sociologia da Infância,

Sandra Marlene; Mendes Barra



Microsoft Academic Search

RESUMO: Propomos uma análise do discurso didático expositivo escrito, em textos do ensino fundamental, focalizando dois aspectos principais. Em primeiro lugar, consideramos o funcionamento cognitivo desse tipo de discurso, com base nas perspectivas de Bruner (1998; 2001) e Bronckart (1999). Em segundo lugar, examinamos algumas de suas principais estruturas semânticas, a partir da abordagem da lingüística cognitiva (Langacker. 1987; 1991;

Luís Passeggi


Automatic localization of the da Vinci surgical instrument tips in 3-D transrectal ultrasound.  


Robot-assisted laparoscopic radical prostatectomy (RALRP) using the da Vinci surgical system is the current state-of-the-art treatment option for clinically confined prostate cancer. Given the limited field of view of the surgical site in RALRP, several groups have proposed the integration of transrectal ultrasound (TRUS) imaging in the surgical workflow to assist with accurate resection of the prostate and the sparing of the neurovascular bundles (NVBs). We previously introduced a robotic TRUS manipulator and a method for automatically tracking da Vinci surgical instruments with the TRUS imaging plane, in order to facilitate the integration of intraoperative TRUS in RALRP. Rapid and automatic registration of the kinematic frames of the da Vinci surgical system and the robotic TRUS probe manipulator is a critical component of the instrument tracking system. In this paper, we propose a fully automatic registration technique based on automatic 3-D TRUS localization of robot instrument tips pressed against the air-tissue boundary anterior to the prostate. The detection approach uses a multiscale filtering technique to identify and localize surgical instrument tips in the TRUS volume, and could also be used to detect other surface fiducials in 3-D ultrasound. Experiments have been performed using a tissue phantom and two ex vivo tissue samples to show the feasibility of the proposed methods. Also, an initial in vivo evaluation of the system has been carried out on a live anaesthetized dog with a da Vinci Si surgical system and a target registration error (defined as the root mean square distance of corresponding points after registration) of 2.68 mm has been achieved. Results show this method's accuracy and consistency for automatic registration of TRUS images to the da Vinci surgical system. PMID:23674418

Mohareri, Omid; Ramezani, Mahdi; Adebar, Troy K; Abolmaesumi, Purang; Salcudean, Septimiu E



The cooperative self-assembly of 25 and 23kDa amelogenins.  


Self-assembly of the extracellular matrix protein amelogenin is believed to play an essential role in regulating the growth and organization of enamel crystals during enamel formation. The full-length amelogenin uniquely regulates the growth, shape, and arrangement of enamel crystals. Protein hydrolysis will ultimately facilitate a tissue with high mineral content. Protein processing is however highly specific suggesting a functional role of the cleaved amelogenins in enamel maturation. Here we hypothesize that the cooperative self-assembly of the recombinant full-length amelogenin 25kDa and the 23kDa proteolytic cleavage product is a function of pH, mixing ratio and incubation time and is associated with the isoelectric point of the protein. Self-assembly of amelogenin into nanospheres which increased in size with increasing pH was observed by atomic force microscopy. Elongated structures of about 100nm length and 25nm width formed over several days for amelogenin 25 and 23kDa predominantly at pH-values of 6.5 and 7.5, respectively. When both proteins 25 and 23kDa were mixed, self-assembled nanostrings of 200-300nm length consisting of fused nanospheres were obtained at pH around 7.0 within 24h. The protein nanostrings formed links over time and a continuous mesh was obtained after 7 days. Electrical conductivity data also showed gradual changes when both amelogenins were mixed in solutions supporting the idea that elongated structures form over extended periods of time. We propose that due to the difference in the isoelectric point, self-assembled nanospheres composed of 23 or 25kDa amelogenin have opposite ionic charges at pH-values around 7.0 and thus experience ionic attraction that enables cooperative self-assembly. PMID:18845261

He, Xiaodong; Li, Wu; Habelitz, Stefan



The cooperative self-assembly of 25 and 23 kDa amelogenins  

PubMed Central

Self-assembly of the extracellular matrix protein amelogenin is believed to play an essential role in regulating the growth and organization of enamel crystals during enamel formation. The full-length amelogenin uniquely regulates the growth, shape, and arrangement of enamel crystals. Protein hydrolysis will ultimately facilitate a tissue with high mineral content. Protein processing is however highly specific suggesting a functional role of the cleaved amelogenins in enamel maturation. Here we hypothesize that the cooperative self-assembly of the recombinant full-length amelogenin 25 kDa and the 23 kDa proteolytic cleavage product is a function of pH, mixing ratio and incubation time and is associated with the isoelectric point of the protein. Self-assembly of amelogenin into nanospheres which increased in size with increasing pH was observed by atomic force microscopy. Elongated structures of about 100nm length and 25 nm width formed over several days for amelogenin 25 and 23 kDa predominantly at pH-values of 6.5 and 7.5, respectively. When both proteins 25 and 23 kDa were mixed, self-assembled nanostrings of 200–300 nm length consisting of fused nanospheres were obtained at pH around 7.0 within 24 h. The protein nanostrings formed links over time and a continuous mesh was obtained after 7 days. Electrical conductivity data also showed gradual changes when both amelogenins were mixed in solutions supporting the idea that elongated structures form over extended periods of time. We propose that due to the difference in the isoelectric point, self-assembled nanospheres composed of 23 or 25 kDa amelogenin have opposite ionic charges at pH-values around 7.0 and thus experience ionic attraction that enables cooperative self-assembly.

He, Xiaodong; Li, Wu; Habelitz, Stefan



Comportamento da dose glandular versus contraste do objeto em mamografia: determinação de formalismo semi-empírico para diferentes combinações alvo-filtro  

Microsoft Academic Search

OBJETIVO: Verificar o efeito da mudança no contraste do objeto, tempo de exposição e dose de radiação quando diferentes espessuras de filtração de molibdênio (Mo) e ródio (Rh) são empregadas em mamógrafos. MATERIAIS E MÉTODOS: Realizaram-se medidas da exposição na entrada da pele com uma câmara de ionização para diferentes espessuras para os filtros de Mo e Rh. Para determinar

Gabriela Hoff; Carlos Eduardo de Almeida; Gary T. Barnes



11 CFR 111.12 - Subpoenas and subpoenas duces tecum; depositions (2 U.S.C. 437d(a) (3), (4)).  

Code of Federal Regulations, 2011 CFR

...subpoenas duces tecum; depositions (2 U.S.C. 437d(a) (3), (4)). 111.12...GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111...subpoenas duces tecum; depositions (2 U.S.C. 437d(a) (3), (4))....



11 CFR 111.13 - Service of subpoenas, orders and notifications (2 U.S.C. 437d(a) (3), (4)).  

Code of Federal Regulations, 2011 CFR

...subpoenas, orders and notifications (2 U.S.C. 437d(a) (3), (4)). 111.13...GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111...subpoenas, orders and notifications (2 U.S.C. 437d(a) (3), (4))....



11 CFR 111.15 - Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)).  

Code of Federal Regulations, 2011 CFR

...Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). 111.15...GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4))....




Microsoft Academic Search

L'approccio comunemente utilizzato per il calcolo teorico del campo di deformazione indotto da una sorgente sismica in un mezzo elastico è basato sull'equivalenza fra una dislocazione di taglio elementare e una doppia coppia di forze puntiformi. Il campo prodotto da una sorgente di dimensioni finite viene poi ottenuto tramite sovrapposizione lineare dei campi generati da una distribuzione di doppie coppie

S. Tinti; A. Armigliato; A. Manucci



Microsoft Academic Search

RESUMO: Este artigo propõe uma análise da imagem da mulher que se esboça em alguns artigos do Código Penal Brasileiro. Para isso, vale mo-nos de algumas orientações teóricas da Análise do Discurso, no que diz respeito a Formaçõe s Discursivas, Ação e Racionalidade e Intencionalidade. Através desse aparato teórico, o trabalho levanta algumas questões de ordem lingüístico-discursivas, fundadoras de um

Carla Roselma; Athayde GUIMARAES; Gustavo Miranda SILVA; Priscilla Chantal; Duarte MARCHESANI


In vitro expression of the 50-kDa and 30-kDa fragments of the P97 adhesin of Mycoplasma hyopneumoniae in Escherichia coli and their use for serodiagnosis  

PubMed Central

This article reports the cloning and expression of 2 fragments of the P97 adhesin of Mycoplasma hyopneumoniae for use in serodiagnosis: a 50-kDa fragment (including the N-terminal cleavage site) and a 30-kDa fragment (including the C-terminal R1 and R2 repeats, which are essential for adherence). The genes encoding the fragments were amplified, cloned, and expressed in the Escherichia coli expression system BL21(DE3)pLysS. Antiserum against the purified recombinant proteins reacted with the mycoplasmal 97-kDa intact protein and the 66-kDa major cleavage fragment, confirming that both cloned fragments could induce antigen-specific antibodies in mice. Of 70 serum samples from nonvaccinated pigs, 26 (37%) were seropositive when the 30-kDa fragment was used as an antigen for enzyme-linked immunosorbent assay, suggesting that natural mycoplasmal infection is quite common in Korea. However, only 4 samples were seropositive when the 50-kDa fragment was used; this fragment was therefore deemed unsuitable for serodiagnosis. The 30-kDa fragment protein might be useful for measuring antibody response to vaccination and for detecting mycoplasmal infection.

Jang, Eun Jin; Kim, Tae Jung



87-kDa protein, a major specific substrate for protein kinase C: purification from bovine brain and characterization  

SciTech Connect

The 87-kDa protein, a major specific substrate for protein kinase C, has been purified 500-fold to apparent homogeneity from bovine forebrain supernatant. The purification procedure included batch adsorption to DE-52 (DEAE-cellulose), (NH/sub 4/)/sub 2/SO/sub 4/ precipitation, and chromatography on DEAE-Sephacel, Bio-Gel HTP (hydroxylapatite), Sephacryl S-400, and fast protein liquid chromatography ProRPC. The amino acid composition was notable for its high proportion of alanine (28.6 mol%) and its enrichment in glutamate/glutamine (18.1 mol%), glycine (12.6 mol%), and proline (11.3 mol%). The partial specific volume was 0.702 ml/g; the Stokes radius and sedimentation coefficient were 85 A and 2.11 S, respectively. Although the relative molecular mass of the protein on NaDodSO/sub 4//8% PAGE was 87-90 kDa, the molecular mass as determined from the above values was 68 kDa. The frictional ratio was 3.2, and the axial ratio was 60, indicating that the 87-kDa protein is an extremely elongated monomer. The purified 87-kDa protein was phosphorylated by purified protein kinase C to a stoichiometry of 2.2 mol of /sup 32/P per mol of 87-kDa protein (calculated using a value of 68 kDa for the molecular mass). Phosphorylation was exclusively on serine residues.

Albert, K.A.; Nairn, A.C.; Greengard, P.



Whole-genome sequences of DA and F344 rats with different susceptibilities to arthritis, autoimmunity, inflammation and cancer.  


DA (D-blood group of Palm and Agouti, also known as Dark Agouti) and F344 (Fischer) are two inbred rat strains with differences in several phenotypes, including susceptibility to autoimmune disease models and inflammatory responses. While these strains have been extensively studied, little information is available about the DA and F344 genomes, as only the Brown Norway (BN) and spontaneously hypertensive rat strains have been sequenced to date. Here we report the sequencing of the DA and F344 genomes using next-generation Illumina paired-end read technology and the first de novo assembly of a rat genome. DA and F344 were sequenced with an average depth of 32-fold, covered 98.9% of the BN reference genome, and included 97.97% of known rat ESTs. New sequences could be assigned to 59 million positions with previously unknown data in the BN reference genome. Differences between DA, F344, and BN included 19 million positions in novel scaffolds, 4.09 million single nucleotide polymorphisms (SNPs) (including 1.37 million new SNPs), 458,224 short insertions and deletions, and 58,174 structural variants. Genetic differences between DA, F344, and BN, including high-impact SNPs and short insertions and deletions affecting >2500 genes, are likely to account for most of the phenotypic variation between these strains. The new DA and F344 genome sequencing data should facilitate gene discovery efforts in rat models of human disease. PMID:23695301

Guo, Xiaosen; Brenner, Max; Zhang, Xuemei; Laragione, Teresina; Tai, Shuaishuai; Li, Yanhong; Bu, Junjie; Yin, Ye; Shah, Anish A; Kwan, Kevin; Li, Yingrui; Jun, Wang; Gulko, Pércio S



Immunological screening and characterization of highly specific monoclonal antibodies against 20 kDa hGH.  


Background: hGH has been widely abused as a doping agent in sports for many years. There are some important approaches for the detection of hGH doping, and the ratio of 22:20 kDa GH was considered one of the most suitable detection indicators of GH abuse. Currently, effective anti-GH antibodies and related reagents are needed to develop a detection method, in particular, highly specific anti-20 kDa hGH monoclonal antibodies are a prerequisite. Herein we constructed the expression vector of 20 kDa hGH and prepared the corresponding antibodies by the immunization of the recombinant human 20 kDa into mice. Positive clones that can specifically recognize 20 kDa hGH were screened and characterized by enzyme immunoassay, Dot-ELISA and surface plasmon resonance. In total, 14 specific monoclonal cell lines were screened out. Results: By a series of characterization, it was found that the 6C8, 44H3, 12G7 and 33Y19 clones were showing much higher specificity and affinity to 20 kDa hGH, and P3H9 could recognize both 20 and 22 kDa hGH isoforms. 6C8 and 44H3 matched well with P3H9 in the surface plasmon resonance testing. The 12G7 clone had the best surface properties with an association constant of 3.4 × 10(9) M(-1) and a dissociation constant of 2.95 × 10(10) M. Conclusion: Highly specific monoclonal antibodies against 20 kDa hGH were generated, and also two paired antibodies (P3H9 and 6C8 or P3H9 and 44H3) were characterized, which can serve as the potential components for 22:20 kDa detection kit. PMID:23013398

Du, Hongwu; Chen, Guangyu; Wang, Shuang; Wang, Shan; Li, Shaoxu; Li, Chuanbao; Xun, Yiping; He, Chunji; Wu, Moutian



16-kDa Prolactin Reduces Angiogenesis, but Not Growth of Human Breast Cancer Tumors In Vivo  

Microsoft Academic Search

Prolactin (PRL) is a peptide hormone necessary for normal growth and development of the human breast. In addition, high levels\\u000a of PRL in plasma correlate with increased risk of breast cancer, especially among postmenopausal women. Several isoforms of\\u000a PRL exist in human circulation, including a 16-kDa isoform that is an N-terminal fragment of the full-length 23-kDa PRL. 16-kDa\\u000a PRL has

Jessica M. Faupel-Badger; Erika Ginsburg; Jodie M. Fleming; Leah Susser; Teresa Doucet; Barbara K. Vonderhaar



O Imperio como Argumento: Um Contraponto entre Joaquim Nabuco e o Bispo D. Jose Mauricio da Rocha (The Empire as Argument: A Counterpoint between Joaquim Nabuco and the Bishop Dom Jose Mauricio da Rocha).  

ERIC Educational Resources Information Center

Dialogs with the previous article establishing a comparative analysis between the political acceptance present in the reactionary speech of Dom Jose Mauricio da Rocha and in the monarchist words of Joaquim Nabuco. (PA)

Marson, Izabel Andrade



Seguranca radiologica no uso de tecnicas nucleares -atividades desenvolvidas pelo Centro de Desenvolvimento da Tecnologia Nuclear - CNEN. (Radiological safety in the use of nuclear technique-activities developed by Centro de Desenvolvimento da Tecnologia Nuclear - CNEN).  

National Technical Information Service (NTIS)

The activities developed by the Centro de Desenvolvimento da Tecnologia Nuclear - CDTN supporting the users of nuclear techniques are described. The methodology used and future plans for the group are shown. (E.G.). (Atomindex citation 22:055424)

T. Wakabayashi E. G. Pereira T. Hirayama



Monodisperse 130 kDa and 260 kDa Recombinant Human Hemoglobin Polymers as Scaffolds for Protein Engineering of Hemoglobin-Based Oxygen Carriers  

PubMed Central

A recombinant 130 kDa dihemoglobin which is made up of a single-chain tetra-? globin and four ? globins has been expressed as a soluble protein in E. coli. The sequence of the single chain tetra-? is: ?I-Gly-?II-(SerGlyGly)5Ser-?III-Gly-?IV. This dihemoglobin has been purified and characterized in vitro by size exclusion chromatography, electrospray mass spectroscopy, equilibrium oxygen binding, and analytical ultracentrifugation. The observed values of P50 and nmax for the dihemoglobin are slightly lower than those observed for the recombinant hemoglobin rHb1.1 (a “monohemoglobin” comprised of two ? globins and an ?I-Gly-?II di?-globin chain). Titration of the deoxy form of dihemoglobin with CO shows that all eight heme centers bind ligand. In vivo, dihemoglobin showed increased circulating halflife and a reduced pressor response in conscious rats when compared to rHb1.1. These observations suggest that dihemoglobin is an oxygen carrying molecule with desirable in vivo properties and provides a platform for an isooncotic hemoglobin solution derived solely from a recombinant source. A 260 kDa tetrahemoglobin has also been produced by chemical crosslinking of a dihemoglobin that contains a Lys16Cys mutation in the C-terminal ?-globin subunit. Tetrahemoglobin also shows reduced vasoactivity in conscious rats that is comparable to that observed for dihemoglobin.

Marquardt, David A.; Doyle, Michael P.; Davidson, Jeffrey S.; Epp, Janet K.; Aitken, Jacqueline F.; Lemon, Douglas D.; Anthony-Cahill, Spencer J.



The same nuclear proteins bind to the 5?-flanking regions of genes for the rice seed storage protein: 16 kDa albumin, 13 kDa prolamin and type II glutelin  

Microsoft Academic Search

Expression of rice seed storage-protein genes is dramatically regulated over a short period of seed maturation. To characterize the expression mechanism of the rice seed storage protein genes, their expression of major storage protein genes (16 kDa albumin, 13 kDa prolamin and type II glutelin) were compared by RNA blot analysis. Their coordinate expression suggests that the transcriptional regulatory machinery

Masayuki Nakase; Takehisa Yamada; Takahiro Kira; Junji Yamaguchi; Naohito Aoki; Ryo Nakamura; Tsukasa Matsuda; Takahiro Adachi



Iron abundance in the hot DA white dwarfs Feige 24 and G191 B2B  

NASA Technical Reports Server (NTRS)

Attention is given to model calculations of the far- and extreme-UV line spectra of highly ionized Fe species (Fe IV, Fe V, and Fe VI) for hot high-gravity H-rich stars. A spectral analysis of 31 hr of exposure of the DA white dwarf Feige 24 with IUE in the echelle mode reveals the presence of Fe with an abundance relative to H by number of (5-10) x 10 exp -6 with an uncertainty dominated by the determination of stellar parameters. An analysis of IUE data from the white dwarf G191 B2B results in a similar Fe abundance if this star shares similar atmospheric parameters (Teff, g) with Feige 24. Fe is thus the second most abundant photospheric element in hot DA white dwarfs.

Vennes, Stephane; Chayer, Pierre; Thorstensen, John R.; Bowyer, Stuart; Shipman, Harry L.



Visual degradation in Leonardo da Vinci's iconic self-portrait: A nanoscale study  

NASA Astrophysics Data System (ADS)

The discoloration of ancient paper, due to the development of oxidized groups acting as chromophores in its chief component, cellulose, is responsible for severe visual degradation in ancient artifacts. By adopting a non-destructive approach based on the combination of optical reflectance measurements and time-dependent density functional theory ab-initio calculations, we describe and quantify the chromophores affecting Leonardo da Vinci's iconic self-portrait. Their relative concentrations are very similar to those measured in modern and ancient samples aged in humid environments. This analysis quantifies the present level of optical degradation of the Leonardo da Vinci's self-portrait which, compared with future measurements, will assess its degradation rate. This is a fundamental information in order to plan appropriate conservation strategies.

Conte, A. Mosca; Pulci, O.; Misiti, M. C.; Lojewska, J.; Teodonio, L.; Violante, C.; Missori, M.



Antigenic secreted proteins from Haemophilus paragallinarum. A 110-kDa putative RTX protein.  


Haemophilus paragallinarum is the causal agent of infectious coryza, an economically important disease for the poultry industry. This bacterium secreted proteins of 25-110 kDa during its growth in brain heart infusion, tryptic soy broth, or Luria-Bertani glucose phosphate media, all lacking serum. Some of these proteins were recognized by sera from chickens experimentally infected with H. paragallinarum. A 110-kDa protein was recognized by a serum pool from convalescent-phase pigs naturally infected with Actinobacillus pleuropneumoniae, and also by a rabbit polyclonal serum against Apx I as well as a rabbit serum against Mannheimia haemolytica leukotoxin, suggesting the presence of an RTX-like protein in H. paragallinarum. H. paragallinarum secreted proteins could be important immunogens in the control of infectious coryza. PMID:15019738

Mena-Rojas, Erika; Vázquez Cruz, Candelario; Vaca Pacheco, Sergio; García González, Octavio; Pérez-Márquez, Víctor M; Pérez-Méndez, Alma; Ibarra-Caballero, Jorge; de la Garza, Mireya; Zenteno, Edgar; Negrete-Abascal, Erasmo



Optimizing IrDA throughput by including processing time with physical layer consideration  

NASA Astrophysics Data System (ADS)

Feature Issue on Optical Wireless Communications (OWC) We derive new equations linking the Infrared Data Association (IrDA) data link layer (IrLAP) throughput to the physical layer by providing the bit error rate in relation to link distance and various physical layer parameters. Furthermore, we optimize the link by simultaneously adapting the frame and window size. The optimum equations include the effect of processor speed. The mathematical analysis is validated by comparison with exact numerical methods. Analytical results presented in the paper explore the effect of frame processing time on improving the performance of IrDA links and identify the improvement in throughput performance by utilizing the derived optimum values.

Huang, Pi; Chatzimisios, P.; Boucouvalas, A. C.



Isolation and characterization of a 22 kDa protein with antifungal properties from maize seeds.  


We have purified a 22 kDa protein from maize seeds to homogeneity by ammonium sulfate precipitation, chitin extraction and Mono-S column chromatography. The purified protein inhibited the growth of the agronomically important pathogens of potato wilt (Fusarium oxysporum) and tomato early blight (Alternaria solani). Sequence analysis of the purified protein showed that it has 52% homology with the sweet protein thaumatin (Edens, L., Hselinga, L., Klok, R., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. (1982) Gene 18, 1-12), 57% homology with the pathogenesis-related protein (Cornelissen, B. J. C., Huijsduijnen, R. A. M., and Bol, J. F. (1986) Nature 321, 531-532) and 99% homology with the 22 kDa trypsin/alpha-amylase inhibitor (Richardson, M., Valdes-Rodriguez, S., and Blanco-Labra, A. (1987) Nature 327, 432-434). PMID:1731773

Huynh, Q K; Borgmeyer, J R; Zobel, J F



Development of noSQL data storage for the ATLAS PanDA Monitoring System  

NASA Astrophysics Data System (ADS)

For several years the PanDA Workload Management System has been the basis for distributed production and analysis for the ATLAS experiment at the LHC. Since the start of data taking PanDA usage has ramped up steadily, typically exceeding 500k completed jobs/day by June 2011. The associated monitoring data volume has been rising as well, to levels that present a new set of challenges in the areas of database scalability and monitoring system performance and efficiency. These challenges are being met with a R&D effort aimed at implementing a scalable and efficient monitoring data storage based on a noSQL solution (Cassandra). We present our motivations for using this technology, as well as data design and the techniques used for efficient indexing of the data. We also discuss the hardware requirements as they were determined by testing with actual data and realistic loads.

Potekhin, M.; ATLAS Collaboration



Analysis of DA White Dwarfs from the McCook & Sion Catalog  

NASA Astrophysics Data System (ADS)

The Sloan Digital Sky Survey has already doubled the number of spectroscopically identified white dwarfs. However, there remains a wealth of white dwarfs in the McCook & Sion catalog for which little or nothing is known. We have thus undertaken a spectroscopic survey of all Northern hemisphere DA white dwarfs from this catalog in order to (1) confirm the spectroscopic classification, and (2) provide measurements of Teff and log g for each star. As part of this project, we have also secured spectroscopic data for all DA stars with high-speed photometric measurements available, in order to study the purity of the ZZ Ceti instability strip, which has recently been challenged, once again.

Gianninas, A.; Bergeron, P.; Dufour, P.



Initial insight into the function of the lysosomal 66.3 kDa protein from mouse by means of X-ray crystallography  

PubMed Central

Background The lysosomal 66.3 kDa protein from mouse is a soluble, mannose 6-phosphate containing protein of so far unknown function. It is synthesized as a glycosylated 75 kDa precursor that undergoes limited proteolysis leading to a 28 kDa N- and a 40 kDa C-terminal fragment. Results In order to gain insight into the function and the post-translational maturation process of the glycosylated 66.3 kDa protein, three crystal structures were determined that represent different maturation states. These structures demonstrate that the 28 kDa and 40 kDa fragment which have been derived by a proteolytic cleavage remain associated. Mass spectrometric analysis confirmed the subsequent trimming of the C-terminus of the 28 kDa fragment making a large pocket accessible, at the bottom of which the putative active site is located. The crystal structures reveal a significant similarity of the 66.3 kDa protein to several bacterial hydrolases. The core ???? sandwich fold and a cysteine residue at the N-terminus of the 40 kDa fragment (C249) classify the 66.3 kDa protein as a member of the structurally defined N-terminal nucleophile (Ntn) hydrolase superfamily. Conclusion Due to the close resemblance of the 66.3 kDa protein to members of the Ntn hydrolase superfamily a hydrolytic activity on substrates containing a non-peptide amide bond seems reasonable. The structural homology which comprises both the overall fold and essential active site residues also implies an autocatalytic maturation process of the lysosomal 66.3 kDa protein. Upon the proteolytic cleavage between S248 and C249, a deep pocket becomes solvent accessible, which harbors the putative active site of the 66.3 kDa protein.

Lakomek, Kristina; Dickmanns, Achim; Kettwig, Matthias; Urlaub, Henning; Ficner, Ralf; Lubke, Torben



A constitutively expressed 36 kDa exochitinase from Bacillus thuringiensis HD1  

Microsoft Academic Search

A 36kDa chitinase was purified by ion exchange and gel filtration chromatography from the culture supernatant of Bacillus thuringiensis HD-1. The chitinase production was independent of the presence of chitin in the growth medium and was produced even in the presence of glucose. The purified chitinase was active at acidic pH, had an optimal activity at pH 6.5, and showed

Naresh Arora; Tarannum Ahmad; R Rajagopal; Raj K Bhatnagar



27 kDa Fasciola gigantica Glycoprotein for the Diagnosis of Prepatent Fasciolosis in Cattle  

Microsoft Academic Search

An indirect enzyme-linked immunosorbent assay (ELISA) using 27 kDa glycoprotein of Fasciola gigantica has been evaluated for its potential use in the diagnosis of bovine fasciolosis. Following experimental infection of rabbits, F. gigantica infection-induced antibodies were isolated and later used as ligands in affinity chromatography for isolation of infection-induced antibody-specific proteins. Among the five infection-specific proteins isolated, a glycoprotein of

S. Ghosh; P. Rawat; R. Velusamy; D. Joseph; S. C. Gupta; B. P. Singh



Separation of glucose oxidase and catalase using ultrafiltration with 300-kDa polyethersulfone membranes  

Microsoft Academic Search

This work examines the separation of glucose oxidase (GOx) and catalase by ultrafiltration using 300kDa MWCO polyethersulfone membranes. The effects of solution pH and ionic strength on the transmission of a single protein (either GOx or catalase) were quantified using the pulsed sample injection technique. Other effects including stirring speed and permeate flux on separation of the binary protein mixtures

Jianguo Liu; Junren Lu; Xiubo Zhao; Jianren Lu; Zhanfeng Cui



A 200 kDa protein is associated with haemagglutinating isolates of Moraxella (Branhamella) catarrhalis  

Microsoft Academic Search

Moraxella catarrhalis adheres to human erythrocytes by means of a proteinaceous, trypsin sensitive, heat modifiable haemagglutinin. A 200 kDa protein was found to be associated with haemagglutinating isolates of M. catarrhalis. This protein was present on all haemagglutinating isolates (n=17), but was absent on the non-haemagglutinating isolates (n=23) examined. This protein demonstrated heat-modifiable properties in sodium dodecyl sulfate and was

Margaret Fitzgerald; Riona Mulcahy; Susan Murphy; Conor Keane; Davis Coakley; Thomas Scott



Peculiarity of Hexamethylenetetratellurafulvalene (HMTTeF) Charge Transfer Complexes of Donor-Acceptor (DA) Type  

Microsoft Academic Search

The complex formation of hexamethylenetetratellurafulvalene (HMTTeF) with 28 kinds of organic electron acceptors yielded 31 charge transfer (CT) complexes. The infrared and ultra-violet–visible–near-infrared spectra of the complexes were examined to study the ionicity of their ground states in solid. A plot of CT transition energies and the difference of redox potentials; ?E(DA) of donor (D) and acceptor (A) molecules indicated

Sang-Soo Pac; Gunzi Saito



Involvement of the 90 kDa glycoprotein in adhesion of Nectria haematococca macroconidia  

Microsoft Academic Search

Previously we demonstrated thatNectria haematococcamacroconidia become adhesive within 5 min and adhere at the spore apices to substrata and to other spores. At the same time, the macroconidia produce mucilage at the spore tips which binds Concanavalin A (Con A), and produce a macroconidial-specific 90 kDa glycoprotein which binds Con A; Con A inhibits macroconidial adhesion. Here we demonstrate that

Y. H Kwon; L Epstein



Purification and properties of an oestrogen-stimulated mouse uterine glycoprotein (approx. 70 kDa).  

PubMed Central

An oestrogen-induced secretory protein from mouse uterine luminal fluid was purified by CM-Affi-Gel Blue chromatography and reverse-phase h.p.l.c. This protein has an apparent molecular mass of approx. 70 kDa both by SDS/polyacrylamide-gel electrophoresis (with or without 2-mercaptoethanol) and by gel-filtration column chromatography, indicating that it exists as a single-chain polypeptide. Further analysis of the protein revealed that it is highly basic (pI greater than or equal to 10) and is a glycoprotein. The N-terminus appears to be blocked to Edman degradation. The partial amino acid sequence of a fragment was obtained by cleavage with CNBr; no sequence homology was apparent between the analysed fragment and other known sequences. The incorporation of [35S]methionine into uterine proteins in vitro revealed that oestrogen treatment of immature mice stimulates both synthesis and secretion of the 70 kDa protein. An enzyme-linked immunosorbent assay with polyclonal antibody was used to determine the tissue distribution of the protein. Tissues such as lung, brain, spleen, muscle, intestine, liver, kidney and ovary of oestrogen-treated mice did not have detectable amounts of the 70 kDa protein. Immunoreactivity was present in uterine and vaginal tissues from oestrogen-treated animals. The 70 kDa protein was not induced by testosterone or progesterone. Although the function of this protein is unknown, it is useful as a marker for the study of oestrogen action in the mammalian uterus as well as regulation of gene expression at the molecular level. Images Fig. 1. Fig. 2. Fig. 3. Fig. 4. Fig. 6.

Teng, C T; Walker, M P; Bhattacharyya, S N; Klapper, D G; DiAugustine, R P; McLachlan, J A



Localization of 70-kDa stress protein induction in gerbil brain after ischemia  

Microsoft Academic Search

Induction of the 70-kDa heat shock protein, hsp70, has been demonstrated in brain following experimental stroke. In the present study, hsp70 was localized in gerbil brain at intervals after transient ischemia using a monoclonal antibody specific for stress-inducible forms of hsp70-related proteins. Induced immunoreactivity was found only in neurons, primarily in hippocampus, striatum, entorhinal cortex and some neocortical regions. Notably

K. Vass; W. J. Welch; T. S. Nowak



Protein 70 kDa in the control of sleep and thermoregulation  

Microsoft Academic Search

Studies of expression of molecular chaperones of the family of Heat Shock Proteins 70 kDa (HSP70) in the mouse and rat brain\\u000a during sleep deprivation do not answer the question whether the HSP70 produce somnogenic effect. In the present work there\\u000a are studied effects of exogenous Hsp70 that is known to be able to penetrate into living cells in vitro

Yu. F. Pastukhov; I. V. Ekimova; K. A. Khudik; I. V. Guzhova



Geological Structure of the Quaternary Sedimentary Sequence in the Klaip?da Strait, Southeastern Baltic  

Microsoft Academic Search

\\u000a The Klaip?da Strait is located between the Curonian Spit and the mainland coast of Lithuania. It links the Curonian Lagoon\\u000a with the Baltic Sea. The Quaternary sequence is represented here by Pleistocene sediments formed during a few glaciations\\u000a and interglacials. Its uppermost part is composed of Late glacial and Holocene sediments originating from different stages\\u000a of the Baltic Sea development.

Albertas Bitinas; Aldona Damušyt?; Anatoly Molodkov


Identification and localization of a 94 kDa membrane protein found in Mycoplasma bovoculi strains  

Microsoft Academic Search

Six isolates of Mycoplasma bovoculi obtained from cattle herds with bovine keratoconjunctivitis were analyzed by gel electrophoresis and immunoblotting techniques. All six strains showed similarity in their protein profiles although no two patterns were identical. Antigenic differences between strains were detected in immunoblots reacted with post-exposure calf serum. A common 94 kDa protein band designated p94 was detected in all

Barik A Salih; Ricardo F Rosenbusch



Microscopic Observations on the Origin of Defects During Machining of Direct Aged (DA) Inconel 718 Superalloy  

Microsoft Academic Search

Surface quality of advanced superalloys after machining is one of the major issues in the aerospace industry because it directly\\u000a affects service characteristics of the machined part. Tool life of cemented carbide inserts with the TiAlN coating during\\u000a machining of direct aged DA 718 alloys under roughing and finishing conditions has been under study. The defect origin on\\u000a the surface

G. K. Dosbaeva; S. C. Veldhuis; A. Elfizy; G. Fox-Rabinovich; T. Wagg



Comparação da sensibilidade de bovinos e búfalos à intoxicação por Palicourea marcgravii (Rubiaceae)1  

Microsoft Academic Search

Barbosa J. D., Oliveira C. M. C., Tokarnia C. H. & Riet-Correa F. 2003. (Comparison of the sensibility of cattle and buffaloes to poisoning by Palicourea marcgravii (Rubiaceae).) Comparação da sensibilidade de bovinos e búfalos à intoxicação por Palicourea marcgravii (Rubiaceae). Pesquisa Veterinária Brasileira 23(4):167-172. Escola de Medicina Veterinária, Campus Castanhal, Universidade Federal do Pará, Rua Maximino Porpino 1000, Castanhal,

José Diomedes Barbosa; Carlos Magno; Chaves de Oliveira; Carlos Hubinger


Photochemical modification of 10 kDa polyethersulfone ultrafiltration membranes for reduction of biofouling  

Microsoft Academic Search

Poly(ether sulfone) 10kDa ultrafiltration membranes were modified by photolysis using ultraviolet light and graft polymerization of hydrophilic monomers onto the membrane surface to create more hydrophilic and lower fouling membrane surfaces. The modified membrane surfaces were characterized by FTIR\\/ATR and captive bubble contact angle measurements to determine chemical and hydrophilicity changes during modification. The modified membranes were compared with an

John Pieracci; James V Crivello; Georges Belfort



Concertação e efetividade da ação política: o Conselho de Desenvolvimento Econômico e Social do governo Lula  

Microsoft Academic Search

A experiência internacional de Conselhos de Desenvolvimento remonta à metade do século passado com a criação dos primeiros conselhos na França (1946\\/1958) e Holanda (1950), vinculando-se à tradição de negociação e representação corporativa que fundamentou a experiência da social-democracia européia. Atualmente também existem conselhos na Áustria (1963), em Portugal (1991\\/1992), na Espanha (1991), na África do Sul (1995), além do

Sonia Fleury



76 FR 56455 - DaVita, Inc.; Analysis of Agreement Containing Consent Orders To Aid Public Comment  

Federal Register 2010, 2011, 2012, 2013

...for confidential treatment, and you have to...for confidential treatment that accompanies...end stage renal disease (``ESRD'') patients receive treatment. In 2010 DaVita's...function of the kidneys by removing...



Model-Independent Determination of Curvature Parameter Using H(z) and DA (z) Data Pairs from BAO Measurements  

NASA Astrophysics Data System (ADS)

We present a model-independent determination of the curvature parameter ? k using the Hubble parameter H(z) and the angular diameter distance DA (z) from recent baryon acoustic oscillation (BAO) measurements. Each H(z) and DA (z) pair from a BAO measurement can constrain a curvature parameter. The accuracy of the curvature measurement improves with increased redshift of H(z) and DA (z) data. By using the H(z) and DA (z) pair derived from a BAO Lyman ? forest measurement at z = 2.36, the ? k is confined to be –0.05 ± 0.06, which is consistent with the curvature of -0.037^{+0.044}_{-0.042} constrained by the nine year Wilkinson Microwave Anisotropy Probe data only. Considering future BAO measurements, at least one order of magnitude improvement of this curvature measurement can be expected.

Li, Yun-Long; Li, Shi-Yu; Zhang, Tong-Jie; Li, Ti-Pei



Geologic and Geotechnical Contributions to the Lagoa DA Rabicha and Cachoeira Mines Projects, Lagoa Real, State of Bahia, Brazil.  

National Technical Information Service (NTIS)

The procedures and systematics involved in obtaining basic geologic-geotechnical, geomechanical and hydrogeotechnical parameters to serve as an aid in the planning of mining activities for the Lagoa da Rabicha and Cachoeira deposits, located in the Lagoa ...

A. A. Tognon J. Costanzo Junior L. M. Ojima T. Oliveira Braga M. Mueller



Diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT: a clinical follow up study  

PubMed Central

The [123I]ioflupane—a dopamine transporter radioligand—SPECT (DaT-SPECT) has proven to be useful in the differential diagnosis of tremor. Here, we investigate the diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT. Therefore, 30 patients with tremor and normal DaT-SPECT were followed up for 2 years. In 18 cases we were able to make a diagnosis. The residual 12 patients underwent a second DaT-SPECT, were then followed for additional 12 months and thereafter the diagnosis was reconsidered again. The final diagnoses included cases of essential tremor, dystonic tremor, multisystem atrophy, vascular parkinsonism, progressive supranuclear palsy, corticobasal degeneration, fragile X–associated tremor ataxia syndrome, psychogenic parkinsonism, iatrogenic parkinsonism and Parkinson's disease. However, for 6 patients the diagnosis remained uncertain. Larger series are needed to better establish the relative frequency of the different conditions behind these cases.

Menendez-Gonzalez, Manuel; Tavares, Francisco; Zeidan, Nahla; Salas-Pacheco, Jose M.; Arias-Carrion, Oscar



A área de proteção ambiental 'Fazenda e Parque do Carmo': os problemas ambientais, as dificuldades de gestão e a influência na qualidade de vida da população The environmental protection area \\  

Microsoft Academic Search

RESUMO: A falta de planejamento urbano da Cidade de São Paulo contribuiu para a eliminação da maior parte da cobertura vegetal, dada à ocupação irregular, inclusive de áreas legalmente protegidas. Essas áreas verdes são reguladoras do equilíbrio ambiental e assim, interferem diretamente na qualidade de vida da população. Através de levantamento bibliográfico e documental e entrevista com o Conselho Consultivo

Mirtes Moreira Silva; José Luís Negrão Mucci; Maria Cecília Focesi Pelicioni


A importância da poeira e ondas de Alfvén na estabilidade de nuvens moleculares anãs  

NASA Astrophysics Data System (ADS)

Nuvens moleculares anãs se apresentam dinamicamente estáveis, embora possuam massas muito maiores que a massa de Jeans. Por este motivo, a estabilidade destes objetos não pode ser explicada considerando-se apenas a pressão térmica. Campos magnéticos, aproximadamente uniformes e de ~mG, exercem um termo extra de pressão que sustenta a nuvem, mas somente na direção perpendicular às linhas de campo. Para a direção paralela, ondas de Alfvén geradas por turbulências no meio, por exemplo, têm sido utilizadas. Estas, sendo supostamente fracamente amortecidas, poderiam sustentar a nuvem nesta direção. Entretanto, estes meios contêm grandes quantidades de poeira carregada eletricamente. Estes grãos de poeira possuem frequências cíclotron, que podem entrar em ressonância com as ondas. Neste trabalho calculamos os efeitos que o amortecimento cíclotron da poeira teriam na propagação da onda, e consequentemente na estabilidade da nuvem. Considerando um fluxo de ondas, com um dado espectro de frequências, e uma população de grãos de poeira, com distribuição de tamanho observada, foi possível mostrar que o amortecimento é eficiente em uma larga banda de frequências. Neste caso as ondas seriam rapidamente amortecidas gerando pequenas condensações de alta densidade, e não poderiam ser utilizadas para explicar a estabilidade de uma nuvem inteira. Desta forma, rotação e turbulência seriam candidatos alternativos para garantir a estabilidade destes objetos.

Falceta-Gonçalves, D.; de Juli, M. C.; Jatenco-Pereira, V.



Microvariabilidade da polarização óptica e do fluxo infravermelho de 3C 279  

NASA Astrophysics Data System (ADS)

Neste trabalho mostramos um estudo sobre a existência e o comportamento da microvariabilidade na polarização óptica no blazar 3C 279. As medidas de polarização foram feitas durante quatro noites em março/2002 com o telescópio de 2.15 m do CASLEO, utilizando o polarímetro CASPROF. As observações mostraram que o grau de polarização linear é composto por duas componentes: uma relativa ao flickering em escalas de tempo de minutos e outra com variações em escalas de tempo de dias. Neste último caso a variação do grau de polarização está correlacionada com variações intraday no infravermelho próximo, cujos dados foram obtidos simultaneamente durante duas noites no telescópio de 1.60 m do LNA, utilizando a CamIV. Além disso, o ângulo de polarização apresentou uma variação súbita de mais de 10 graus em algumas horas, o que poderia ser causado pela injeção de um novo choque no jato. Esta idéia é apoiada pelo comportamento da variabilidade no infravermelho em escalas de tempo mais longas, que foi acompanhado durante três meses após o término da campanha coordenada com observações adicionais no LNA.

Andruchow, I.; Cellone, S. A.; Romero, G. E.; Dominici, T. P.; Abraham, Z.



Identification of a 24 kDa excretory secretory protein in Anisakis simplex.  


A gene coding for a 24 kDa protein (22 U homologous; As22U) was isolated from the Anisakis simplex third-stage larvae cDNA library during expressed sequence tag analysis. As22U was 636 bp long, and was found to code for 212 amino acid residues with a calculated mass of 23.5 kDa and a PI of 9.06. The As22U deduced amino acid sequence harbored a signal peptide region and 16 highly conserved cysteine residues, and it was identified in both the total extracts and excretory secretory (ES) protein of A. simplex. Its molecular weight was measured at 24 kDa via western blot analysis. The expression levels of thymic stromal lymphopoietin, IL-25, and CXCL1 (Gro-?) genes were increased at 6h after recombinant As22U treatment in mouse intestinal epithelial cells. Additionally, thymus and activation-regulated chemokine gene levels were increased at 14 h after treatment. Although we do not currently have sufficient evidence to determine whether As22U plays a role as an allergen, this remains possible. Further in vivo studies may provide some insight as to the allergenic properties of As22U. PMID:22001446

Park, Ji Seoung; Cho, Min Kyoung; Yu, Hak Sun; Ahn, Soon Cheol



Microscopic Observations on the Origin of Defects During Machining of Direct Aged (DA) Inconel 718 Superalloy  

NASA Astrophysics Data System (ADS)

Surface quality of advanced superalloys after machining is one of the major issues in the aerospace industry because it directly affects service characteristics of the machined part. Tool life of cemented carbide inserts with the TiAlN coating during machining of direct aged DA 718 alloys under roughing and finishing conditions has been under study. The defect origin on the surface of the machined part was investigated. Metallographic observations of the DA 718 were made using optical metallography and SEM/EDS. To find out the origins of surface defect formation, the morphology of machined parts and cross sections of the machined surfaces have been investigated. Two major categories of defects were detected on the surface of the machined part: cracks and tears. The origin of the cracks on the machined surface is related to shearing of the primary complex TiC/NbC carbide revealed in a structure of DA 718 alloy. At the same time, Nb-rich regions of the primary complex carbide interact with the environment (oxygen from air) during machining with further formation of low strength oxide layer on the surface, forming tears.

Dosbaeva, G. K.; Veldhuis, S. C.; Elfizy, A.; Fox-Rabinovich, G.; Wagg, T.



Surgical Outcomes of Congenital Atrial Septal Defect Using da VinciTM Surgical Robot System  

PubMed Central

Background Minimally invasive cardiac surgery has emerged as an alternative to conventional open surgery. This report reviews our experience with atrial septal defect using the da VinciTM surgical robot system. Materials and Methods This retrospective study included 50 consecutive patients who underwent atrial septal defect repair using the da VinciTM surgical robot system between October 2007 and May 2011. Among these, 13 patients (26%) were approached through a totally endoscopic approach and the others by mini-thoracotomy. Nineteen patients had concomitant procedures including tricuspid annuloplasty (n=10), mitral valvuloplasty (n=9), and maze procedure (n=4). The mean follow-up duration was 16.9±10.4 months. Results No remnant interatrial shunt was detected by intraoperative or postoperative echocardiography. The atrial septal defects were mainly repaired by Gore-Tex patch closure (80%). There was no operative mortality or serious surgical complications. The aortic cross clamping time and cardiopulmonary bypass time were 74.1±32.2 and 157.6±49.7 minutes, respectively. The postoperative hospital stay was 5.5±3.3 days. Conclusion The atrial septal defect repair with concomitant procedures like mitral valve repair or tricuspid valve repair using the da VinciTM system is a feasible method. In addition, in selected patients, complete port access can be helpful for better cosmetic results and less musculoskeletal injury.

Kim, Ji Eon; Jung, Sung-Ho; Kim, Gwan Sic; Kim, Joon Bum; Choo, Suk Jung; Chung, Cheol Hyun



A new fibrinolytic enzyme (55?kDa) from Allium tuberosum: purification, characterization, and comparison.  


Chives have been used both as food and as medicine. Previously, two fibrinolytic enzymes, ATFE-I (90 kDa) and ATFE-II (55 kDa), were identified in chives (Allium tuberosum), a perennial herb. In the present work, ATFE-II was purified by ion-exchange chromatography followed by gel filtration. In addition, the enzyme properties of ATFE-I and ATFE-II were compared. The molecular mass and isoelectric point (pI value) of ATFE-II were 55 kDa and pI 4.0, respectively, as revealed using one- or two-dimensional fibrin zymography. ATFE-II was optimally active at pH 7.0 and 45°C. ATFE-II degraded the A?-chain of human fibrinogen but did not hydrolyze the B?-chain or the ?-chain, indicating that the enzyme is an ?-fibrinogenase. The proteolytic activity of ATFE-II was completely inhibited by 1 mM leupeptin, indicating that the enzyme belongs to the cysteine protease class. ATFE-II was also inhibited by 1 mM Fe²(+). ATFE-II exhibited high specificity for MeO-Suc-Arg-Pro-Tyr-p-nitroaniline (S-2586), a synthetic chromogenic substrate of chymotrypsin. Thus proteolytic enzymes from A. tuberosum may be useful as thrombolytic agents. PMID:20954802

Chung, Dong-Min; Choi, Nack-Shick; Chun, Hyo Kon; Maeng, Pil Jae; Park, Sang-Bong; Kim, Sung-Ho



Identification of a novel 19 kDa Echinococcus granulosus antigen.  


By screening an Echinococcus granulosus cDNA library with IgG4 from patients with active cystic echinococcosis (CE), we identified a cDNA encoding a protein of 19.0 kDa (Eg19). Eg19, in 12% SDS-PAGE in reducing and non-reducing conditions, showed several bands between 19 and 100 kDa. Immunoblotting (IB) analysis detected total IgG, IgG1 and IgG4 specific to the 38/40 kDa band of Eg19 in the 10% of patients' sera. The percentage of total IgG, IgG1 and IgG4-positive sera were significantly higher in sera from patients with active disease and cyst in multiple sites than from patients with inactive disease and cyst in the liver (P<10(-4)). ELISA analysis disclosed that during the follow-up anti-Eg19 antibody concentration decreased over the course of treatment in sera from patients with cured disease. Even if Eg19 appear to have no benefit in the diagnosis of the disease, our data, confirming the presence of antigens inducing both IgG1 and IgG4 during active development of CE, suggest that Eg19 might be a marker of disease status. PMID:19769934

Delunardo, Federica; Ortona, Elena; Margutti, Paola; Perdicchio, Maurizio; Vacirca, Davide; Teggi, Antonella; Sorice, Maurizio; Siracusano, Alessandra



A 23-kDa protein as a substrate for protein kinase C in bovine neutrophils. Purification and partial characterization  

Microsoft Academic Search

In ³²P{sub i}-loaded bovine neutrophils stimulated with phorbol myristate acetate (PMA), radioactivity was preferentially incorporated into a protein of low molecular mass, suggesting a PKC-dependent phosphorylation. This protein, termed 23-kDa protein, was predominantly localized in the cytosol. The apparent molecular mass of the purified protein range between 20 and 23 kDa. In the absence of mercaptoethanol, a dimer accumulated. Homogeneity

Marie Jose Stasia; Anne Christine Dianoux; Pierre V. Vignais



Subcellular localization of the 28 kDa protein of the triple- gene-block of bamboo mosaic potexvirus  

Microsoft Academic Search

Open reading frame 2 of the bamboo mosaic potexvirus (BaMV) genome encodes a 28 kDa pro- tein, the first of the 'triple-gene-block' of BaMV which is believed to play a role in cell-to-cell movement of the virus in host plants. The 28 kDa protein was expressed in Escherichia coli and polyclonal antiserum was raised in a rabbit. Western blot analyses

Ban-Yang Chang; Na-Sheng Lin; Dann-Ying Liou; Jia-Perng Chen; Gunn-Guang Liou; Yau-Heiu Hsu


Relocalization of an 82-kDa protein from lampbrush loops into the nucleoskeleton during amphibian oogenesis  

Microsoft Academic Search

Summary Affinity-purified antibodies directed against an 82-kDa oocyte nuclear protein ofPleurodeles waltl (Amphibia, Urodela) were prepared using antigen bound to nitrocellulose paper. The specificity of the antibody was controlled on two-dimensional electrophoretic gels of nuclear proteins. The intranuclear distribution of the 82-kDa protein was analyzed by the indirect immunofluorescence method on spreads of oocyte nuclear content. Localization of the protein

Nicole Moreau; Nicole Angelier; Nicole Lautredou



The Portuguese naturalist Correia da Serra (1751–1823) and his impact on early nineteenth-century botany  

Microsoft Academic Search

This paper focuses on the contributions to natural history, particularly in methods of plant classification of the Portuguese\\u000a botanist, man of letters, diplomat, and Freemason Abbé José Correia da Serra (1751–1823), placing them in their national and\\u000a international political and social contexts.\\u000a \\u000a Correia da Serra adopted the natural method of classification championed by the Frenchman Antoine-Laurent de Jussieu, and\\u000a introduced

Maria Paula Diogo; Ana Carneiro; Ana Simões



Purification, immunological characterization and cDNA cloning of a 47 kDa glycoprotein secreted by carrot suspension cells  

Microsoft Academic Search

A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted by a specific subset of non-embryogenic cells.

Fred A. van Engelen; Anke J. de Jong; Ellen A. Meijer; Cor W. Kuil; J. Kees Meyboom; Wim G. Dirkse; Hilbert Booij; Marijke V. Hartog; Joël Vandekerckhove; Sacco C. de Vries; Ab van Kammen



Identification and purification of a novel 120-kDa protein that recognizes the cAMP-responsive element  

SciTech Connect

The TGACGTCA (CRE) motif required for function by a number of cellular (somatostatin, enkephalin, alpha-human chorionic gonadotropin) and viral (Ad5 E1A-inducible, HTLV-1 TAX-inducible) genes is the site of interaction of multiple sequence-specific complexes. A protocol has been developed for the fractionation and purification of these activities. We report here the purification from HeLa nuclear extracts of a novel 120-kDa polypeptide which by Southwestern blots, gel retardation, and UV cross-linking assays displays CRE-specific binding. The CRE-affinity purified 120-kDa protein displays properties distinct from those of the 43-kDa CREB/ATF polypeptide. The 120-kDa protein is readily phosphorylated in vitro by protein kinase C but not by protein kinase A, suggesting that this molecule may mediate cellular signals distinct from the cAMP-responsive pathway. In vitro transcription-complementation assays utilizing the purified 120-kDa protein failed to transactivate the cAMP-responsive somatostatin promoter suggesting that the mode of action of this 120-kDa polypeptide may require an activation step distinct from the cAMP-signaling pathway.

Andrisani, O.; Dixon, J.E. (Purdue Univ., West Lafayette, IN (USA))



Projeto educação em ciências com observatórios virtuais: a participação da Escola Moppe no período 2000-2003  

NASA Astrophysics Data System (ADS)

O projeto Educação em Ciências com Observatórios Virtuais foi concebido pelo Instituto Astronômico e Geofísico da USP, agregando diversas instituições de ensino e pesquisa no país para desenvolver competências diversas na educação fundamental, média e superior utilizando a astronomia como ferramenta multidisciplinar. Este trabalho descreve a participação da MOPPE, escola-piloto do INPE no projeto, no período de 2000-2003. Serão apresentadas 1) a criação de um clube de ciências (1999 a 2001) cujo tema foi a colonização de Marte e 2) a ementa de astronomia trabalhada com as 7a. e 8a. séries do ensino fundamental. A proposta do projeto Colonizando Marte foi estudar diversos aspectos de uma missão interplanetária e construir experiementos que permitissem quantificar esses aspectos. Os resultados obtidos incluiram apresentações nas SBPC Jovem em 2000 e 2001. Discutiremos também as ementas de astronomia trabalhadas desde 2001 e o envolvimento dos alunos com atividades ligadas a astronomia, fora da aula de ciências. A inclusão de astronomia no currículo das últimas séries motivou a participação de mais alunos culminando com a conquista de duas medalhas para alunos da 7a. série na Olimpiada Brasileira de Astronomia em 2002. Houve também um aumento no número de participantes na OBA 2003 e nos projetos de astronomia mais elaborados nas Feira de Ciências de 2001 e 2002. Destacamos em 2003 a inclusão da MOPPE no projeto TIE - Telescopes in Education - da NASA, que utiliza o telescópio do Observatório de Mount Wilson (EUA) para observações remotas em projetos pedagógicos para o ensino de astronomia.

Wuensche, C. A.; Gavioli, E.; Oliveira, A. L. P. R. S.; da Silva, C.; Cardoso, H. P.; Estácio, S.



Effect of DA-8159, a Selective Phosphodiesterase Type 5 Inhibitor, on Electroretinogram and Retinal Histology in Rabbits  

PubMed Central

DA-8159, a selective inhibitor of phosphodiesterase type 5, was developed as a new drug for erectile dysfunction. The effect of DA-8159 on the electroretinogram (ERG) and the retinal histopathology were evaluated in rabbits. The ERG was performed prior to, and 1 and 5 hr after DA-8159 (5 to 30 mg/kg) administration. The plasma concentration of DA-8159 was determined at each time point, and retinal microscopic examination was also performed. There was no statistically significant ERG change at any dose or at any time. Though the 30 Hz flicker showed a prolongation of the implicit time at 5 hr after the administration of either DA-8159 15 mg or 30 mg/kg (p<0.05), but concurrent amplitude decreases were not statistically significant. At a dose of 5 mg/kg, no test drug was detected in the blood after either 1 or 5 hr. At either 15 mg/kg or 30 mg/kg, there was a dose-dependent increase in the blood concentration after 1 hr of drug administration, which decreased with time. In light and electron microscopic examinations of the retina, there was no remarkable change at any dose. These results suggest DA-8159 has a low risk potential to the retina, but further evaluation on the visual functions in human is needed.

Kang, Kyung Koo; Ahn, Gook Jun; Shim, Hyun Joo; Kim, Won Bae



Análise Temporal da Coma de CO+ no Cometa P/Halley  

NASA Astrophysics Data System (ADS)

Observações fotográficas e fotoelétricas da coma de gás ionizado do cometa P/Halley a nível de CO+ em 4250 angstroms fizeram parte do programa de Monitoramento do Halley desenvolvido pela Universidade de Bochum (Ruhr-Universitaet Bochum) na Alemanha, realizado de 17 de fevereiro a 17 de abril de 1986 no European Southern Observatory (ESO) em La Silla (Chile). Nesta faixa espectral é possível observar a contínua formação, bem como o movimento e expansão das estruturas de plasma. Para observar a morfologia destas estruturas foram analisadas 32 placas fotográficas de CO+ (placas de vidro) do cometa P/Halley. Tais placas possuem um campo de visão de 28,6 por 28,6 graus sendo obtidas entre 29 de março e 17 de abril de 1986 com tempos de exposição entre 20 e 120 minutos. Todas as placas foram digitadas com o auxílio de um microdensitômetro PDS 2020 GM (Photometric Data System) do Instituto Astronómico da Westfaelischen Wilhelms-Universitaet em Muenster, Alemanha (um pixel = 25 por 25 micrômetros correspondendo aproximadamente a 46,88 por 46,88 segundos de arco). Após a digitação os dados foram reduzidos à intensidades relativas, sendo que os posssíveis de calibração também foram reduzidos à intensidades absolutas, expressas em termos de densidade colunar utilizando-se dos sistemas de tratamento de imagens MIDAS (Munich Image Data Analysis System; ESO - Image Processing Group, 1988) e IHAP (Image Handling And Processing; Middleburg, 1983). Com o auxílio do método de teta mínimo de Stellingwerf (Stellingwerf, 1978) obteve-se um período de 2,22 +/- 0,09 dias a partir da análise de estruturas na coma de plasma através da subtração de imagens subsequentes. Este método foi comparado com o método de Fourier. Provavelmente exista um segundo ciclo com período aproximado de 3,6 dias. A idéia de subtrair imagens subsequentes é devido ao fato de que os efeitos de rotação são apenas 10% dos fenômenos de distribuição gasosa. Portanto as imagens de diferença são usadas para suprimir a componente estática da nuvem de gás. - ESO - Image Processing Group, 1988, in: MIDAS Manual, ESO, Garching. - Middleburg F., 1983, in: IHAP Manual, ESO, Garching. - Stellingwerf R.F., 1978, ApJ 224, 953.

Voelzke, M. R.; Schlosser, W.; Schmidt-Kaler, Th.



The Greek Database of Seismogenic Sources (GreDaSS): the new version.  

NASA Astrophysics Data System (ADS)

The Greek Database of Seismogenic Sources (GreDaSS) is a repository of geological, tectonic and active fault data for the Greek territory and its surroundings including sectors of western Anatolia, southern Bulgaria, FYR of Macedonia and Albania. The principal aim of this international project is to create a homogenized framework of all data relevant to the seismotectonics, and especially the seismic hazard assessment, of Greece and its surroundings as well as a common research platform for performing seismic hazard analyses, modelling and scenarios from specific seismogenic structures. GreDaSS represents an on-going project based on the results of decades of investigations by the GreDaSS Working Group* and by numerous other researchers working on the active tectonics of the broader Aegean Region. Members of the WG have contributed in several national and European projects like FAUST (Faults as a seismological tool, 1998-2000) and SHARE (Seismic Hazard and Armonization in Europe, 2009-2012). The database is continuously updated by the WG, with the aim of improving its completeness in terms of seismogenic sources analysed and included, but also of enriching and better defining all parametric and ancillary information on each source. Preliminary and partial versions of GreDaSS have been already presented at previous congresses (Pavlides et al., 2010; Caputo et al., 2012; Sboras et al., 2013), but at next EGU the whole area and the most recent version of the database will be displayed. *) the principal scientific contributors belonging to the GreDaSS WG are: Sboras. S., Chatzipetros A., Koukouvelas I., Michailidou A., Valkaniotis S., Tarabusi G. Caputo R., Chatzipetros A., Pavlides S. and Sboras S. (2012): Ann. Geophysics, 55(5), 859-894, doi: 10.4401/ag-5168 Pavlides S., Caputo R., Sboras S., Chatzipetros A., Papathanasiou G. & Valkaniotis S. (2010): Bull. Geol. Soc. Greece, 43(1), 486-494 Sboras S., Pavlides S., Caputo R., Chatzipetros A., Michailidou A., Valkaniotis S. and Papathanasiou G. (2013): Boll. Geofis. Teor. Appl., 13 pp. doi: 10.4430/bgta0101.

Caputo, Riccardo; Pavlides, Spyros; GreDaSS Working Group



El galanteo en la adolescencia. Una El galanteo en la adolescencia. Una El galanteo en la adolescencia. Una El galanteo en la adolescencia. Una El galanteo en la adolescencia. Una apr apr apr apr aproximación desde una perspectiva de géner oximación desde una perspectiva de géner oximación desde una perspectiva de géner oximación desde una perspectiva de géner oximación desde una perspectiva de género  

Microsoft Academic Search

Courtship is the phenomenon that starts the complex link between couples. It is mediated by the social representation of a man or woman for each culture and society. The aim of the present study is to demonstrate the importance of gender analysis for understanding the perpetuity of sexist patterns ant to determine the gender differences that influence the way in

Keytel García Rodríguez


Nuclear 82-kDa choline acetyltransferase decreases amyloidogenic APP metabolism in neurons from APP/PS1 transgenic mice.  


Alzheimer disease (AD) is associated with increased amyloidogenic processing of amyloid precursor protein (APP) to ?-amyloid peptides (A?), cholinergic neuron loss with decreased choline acetyltransferase (ChAT) activity, and cognitive dysfunction. Both 69-kDa ChAT and 82-kDa ChAT are expressed in cholinergic neurons in human brain and spinal cord with 82-kDa ChAT localized predominantly to neuronal nuclei, suggesting potential alternative functional roles for the enzyme. By gene microarray analysis, we found that 82-kDa ChAT-expressing IMR32 neural cells have altered expression of genes involved in diverse cellular functions. Importantly, genes for several proteins that regulate APP processing along amyloidogenic and non-amyloidogenic pathways are differentially expressed in 82-kDa ChAT-containing cells. The predicted net effect based on observed changes in expression patterns of these genes would be decreased amyloidogenic APP processing with decreased A? production. This functional outcome was verified experimentally as a significant decrease in BACE1 protein levels and activity and a concomitant reduction in the release of endogenous A?1-42 from neurons cultured from brains of AD-model APP/PS1 transgenic mice. The expression of 82-kDa ChAT in neurons increased levels of GGA3, which is involved in trafficking BACE1 to lysosomes for degradation. shRNA-induced decreases in GGA3 protein levels attenuated the 82-kDa ChAT-mediated decreases in BACE1 protein and activity and A?1-42 release. Evidence that 82-kDa ChAT can enhance GGA3 gene expression is shown by enhanced GGA3 gene promoter activity in SN56 neural cells expressing this ChAT protein. These studies indicate a novel relationship between cholinergic neurons and APP processing, with 82-kDa ChAT acting as a negative regulator of A? production. This decreased formation of A? could result in protection for cholinergic neurons, as well as protection of other cells in the vicinity that are sensitive to increased levels of A?. Decreasing levels of 82-kDa ChAT due to increasing age or neurodegeneration could alter the balance towards increasing A? production, with this potentiating the decline in function of cholinergic neurons. PMID:24844149

Albers, Shawn; Inthathirath, Fatima; Gill, Sandeep K; Winick-Ng, Warren; Jaworski, Ewa; Wong, Daisy Y L; Gros, Robert; Rylett, R Jane



Structure of yeast pGKL 128-kDa killer-toxin secretion signal sequence. Processing of the 128-kDa killer-toxin-secretion-signal-alpha-amylase fusion protein.  


The linear double-stranded DNA plasmid pGKL1 in yeast encodes a killer toxin consisting of 97-kDa, 31-kDa and 28-kDa subunits. A 128-kDa protein precursor of the 97-kDa and 31-kDa subunits, was first synthesized with a 29-amino-acid extension at its NH2-terminus as a secretion signal sequence. In the present study, the property of this signal sequence was studied by the analysis of a fusion protein with mouse alpha-amylase. Using the secretion signal sequence of the killer protein, the mouse alpha-amylase was successfully secreted into the culture medium. An intracellular precursor form of alpha-amylase was identified and purified. Analysis of the NH2-terminal sequence of this precursor molecule indicated that it corresponded to the secretory intermediate (pro form) of alpha-amylase with the removal of the hydrophobic segment (Met1-Gly16) of the secretion signal. Both the secretion of alpha-amylase into the culture medium and the detection of the pro-alpha-amylase species in the cells were prohibited by a sec 11 mutation, or by the conversion of Gly to Val at the 16th position of the secretion signal. These results strongly suggest that the cleavage occurs between Gly16 and Leu17 by a signal peptidase, and that this cleavage is required for the secretion of alpha-amylase into the medium. Based on the data from the NH2-terminal amino acid sequences of secreted alpha-amylases, we conclude that the 29-amino-acid secretion signal present in the 128-kDa killer toxin precursor protein is a prepro structure. PMID:1735428

Tokunaga, M; Kawamura, A; Omori, A; Hishinuma, F



Relationship of two ribonucleases with molecular masses of 45 kDa and 37 kDa from the culture medium of Lentinus edodes.  


Lentinus edodes (shiitake) produces three base non- specific and acid ribonucleases, RNase Le2, RNase Le37 and RNase Le45. The primary structures of the former two RNases, having molecular masses about 24 and 37 kDa, respectively, have been elucidated to be members of the RNase T2 family. The latter two are excreted from mycelia into the medium. In this report, we estimated the primary structure of RNase Le45 using the following experimental evidence. (i) The partial amino acid sequence of RNase Le45 determined that up to about 60% of total protein was identical with that of RNase Le37. (ii) The amino acid composition of RNase Le45 was identical to that of RNase Le37. (iii) The elution profiles on HPLC of lysylendopeptidase and Staphylococcus aureus V8 protease digests of RCM-RNase Le45 (reduced and S-carboxymethylated RNase Le45) were very similar to those of RNase Le37, except for the absence of C-terminus peptide which contained O-glycosylated peptides. However, RNase Le45 contained about 70 residues of mannose and 4 residues of hexosamine. These values were more than twice those of RNase Le37. (iv) RNase Le45 was immunologically indistinguishable from RNase Le37. (v) After treatment with both glycosidase EndoH and alpha-mannosidase, RNases Le37 and Le45 gave complex bands by slab-gel electrophoresis. However, one of the major bands with the highest mobility from RNase Le45 and Le37 showed the molecular mass of 29 kDa in common, which is slightly larger than that of RNase Le2 containing no carbohydrate. These results indicated that RNase Le45 is an enzyme which is a heavily glycosylated species of RNase Le37. PMID:10919355

Kobayashi, H; Hara, J; Itagaki, T; Inokuchi, N; Koyama, T; Sanda, A; Iwama, M; Ohgi, K; Irie, M



Volume balance of a submarine landslide in the Espírito Santo Basin, offshore Brazil: Quantifying seafloor erosion, sediment accumulation and depletion  

NASA Astrophysics Data System (ADS)

Degrees of seafloor erosion, sediment accumulation and depletion are quantified using a 3D seismic volume of a small submarine landslide offshore Brazil. In the study area, the upper boundaries of large remnant blocks — kept in situ during the main instability event — and the interpreted non-failed margins of the landslide comprise a reliable estimate for the position of the seafloor at the time the submarine landslide was generated. Remnant blocks of strata show little internal deformation, particularly at their base, and were kept upright during a principal instability event triggered in response to regional halokinesis and associated overburden faulting. They are laterally bounded by a mixed succession of mass-wasted chaotic strata, rafted blocks and post-slide sub-horizontal units. Despite the thin accumulation of failed strata now visible, the interpreted data indicate that submarine landslides of small areal dimensions can erode substantial volumes of seafloor sediment. Consequently, the more than 300 m of eroded seafloor strata offshore Brazil is of a similar scale to the largest failures recorded on continental margins (e.g. Storegga Slide; Amazon Fan complex; Cape Fear slide). The scale relationships presented in this paper indicate that the magnitude of seafloor erosion experienced during instability events may not be recorded by distal mass-wasted strata, which can be demonstrably thinner than the original volume of failed sediment. Thus, we conclude that the relative expression of accumulated slide strata is not directly related to the original volume of failed material. In addition, small-scale landslides can be responsible for the erosion of thick seafloor deposits, a factor that can potentially cause significant losses of seal capacity in overburden successions due to the sudden release of confining pressures in a vertical direction.

Alves, Tiago M.; Cartwright, Joseph A.



L'Astronomia del Venerdí Santo, l'eclissi di Luna e l'ora della Sindone  

NASA Astrophysics Data System (ADS)

Dating the crucifixion on friday 3 April 33, when, at sunset a partially eclipsed Moon rised allows to interprete the speech of Saint Peter (Acts 2:20=Joel 3:4) on the day of Pentecost as including an account of this eclipse, as fulfillment of the Scriptures. Few minutes later the apparition of a third star of medium magnitude stated the beginning of the sabbatical rest: before that time Jesus has been buried, wrapped into the shroud. The observations of the equinoctial moonrise from the Mount of Olives on September 2013 are strongly in favour of the hypotehsis of possible observation of the lunar eclipse of 33 AD by Jewish people, as well as the sunrises observed from the Temple Institute terrace in Southern-East Jerusalem demonstrated that this eclipse could have been seen from Jerusalem. The depression of the horizon and the refraction allow to see Sun and Moon more than 1 degree below the geometrical horizon in the azimuthal direction ranging from 90 to 116, prologing to 16 minutes the duration of the partial lunar eclipse observed in 33AD. Texts of Matthew, Mark and Cyrillus of Jerusalem are also commented.

Sigismondi, Costantino



Integrated assessment for establishing an oil environmental vulnerability map: case study for the Santos Basin region, Brazil.  


The growth of maritime transport and oil exploitation activities may increase the risk of oil spills. Thus, plans and actions to prevent or mitigate impacts are needed to minimize the effects caused by oil. However, tools used worldwide to support contingency plans have not been integrated, thus leading to failure in establishing priority areas. This investigation aimed to develop indices of environmental vulnerability to oil (IEVO), by combining information about environmental sensibility to oil and results of numerical modeling of spilled oil. To achieve that, a case study concerning to oil spills scenarios in a subtropical coastal area was designed, and IEVOs were calculated and presented in maps, in order to make the information about the areas' vulnerability more easily visualized. For summer, the extension of coastline potentially affected by oil was approximately 150 km, and most of the coastline presented medium to high vulnerability. For winter, 230 km coastline would be affected, from which 75% were classified as medium to high vulnerability. Thus, IEVO maps allowed a rapid and clearer interpretation of the vulnerability of the mapped region, facilitating the planning process and the actions in response to an oil spill. PMID:23896407

Romero, A F; Abessa, D M S; Fontes, R F C; Silva, G H



The Future Subjunctive in Galician-Portuguese: A Review of "Cantigas de Santa Maria" and "A Demanda do Santo Graal"  

ERIC Educational Resources Information Center

The usage and disappearance of the Central Ibero-Romance future subjunctive have been extensively researched through Old Spanish texts. Studies on the future subjunctive as it evolved in the farther Western Ibero-Romance languages, represented by Galician and Portuguese, have been scarce, if not incomplete. This dissertation partially fills the…

Schultheis, Maria Luiza Carrano



Lysosomal responses as a diagnostic tool for the detection of chronic petroleum pollution at Todos os Santos Bay, Brazil  

Microsoft Academic Search

Coastal marine environments, especially semienclosed systems such as bays, are under unrelenting stress caused by urban and industrial development. Biomonitoring plays a vital role in strategies to identify, assess, and control stressors. However, due to the magnitude of the challenge there is a demand for new and innovative approaches to provide timely and accessible information to environmental managers and policy

L. K. P. Martins; I. A. Nascimento; G. Fillmann; R. King; A. J. A. Evangelista; J. W. Readman; M. H. Depledge



Analyzing the relation between the microbial diversity of DaQu and the turbidity spoilage of traditional Chinese vinegar.  


Vinegar is a traditional fermented condiment, and the microbial diversity of DaQu makes the quality of vinegar products. Recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. In this study, the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar was analyzed by plating technique, PCR-denaturing gradient gel electrophoresis (DGGE), and high-performance liquid chromatography (HPLC). The 16S rRNA sequencing and DGGE analysis indicated that Bacillus (Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus thuringiensis) and Lactobacillus (including Lactobacillus acidipiscis and Lactobacillus pobuzihii) species were the dominant contaminants in vinegar products. Meanwhile, DGGE analysis showed that the dominant bacteria in DaQu belonged to genera Bacillus, Lactobacillus, Pseudomonas, Weissella, Saccharopolyspora, Enterobacter, and Pantoea. However, only two yeast species (Pichia kudriavzevii and Saccharomycopsis fibuligera) and seven mold species including Aspergillus oryzae, Aspergillus niger, Aspergillus candidus, Rhizopus microspores, Eurotium herbariorum, Absidia corymbifera, and Eupenicillium javanicum were detected in the DaQu. The population level of fungi was below 5 log CFU/g in DaQu. The chemical and physical properties of vinegar and sediments were also determined. On the basis of a combined microbial diversity-chemical analysis, we demonstrated that turbidity spoilage of vinegar was a result of cooperation among the low population level and abundance of fungal species in DaQu, the suitable climate conditions, and the contaminants in vinegar. This is the first report to analyze the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar. PMID:24691870

Li, Pan; Li, Sha; Cheng, Lili; Luo, Lixin



Heparin Binds 8 kDa Gelsolin Cross-?-Sheet Oligomers and Accelerates Amyloidogenesis by Hastening Fibril Extension†  

PubMed Central

Glycosaminoglycans (GAGs) are highly sulfated linear polysaccharides prevalent in the extracellular matrix, and they associate with virtually all amyloid deposits in vivo. GAGs accelerate the aggregation of many amyloidogenic peptides in vitro, but little mechanistic evidence is available to explain why. Herein, spectroscopic methods demonstrate that GAGs do not affect the secondary structure of the monomeric 8 kDa amyloidogenic fragment of human plasma gelsolin. Moreover, monomerized 8 kDa gelsolin does not bind to heparin under physiological conditions. In contrast, 8 kDa gelsolin cross-?-sheet oligomers and amyloid fibrils bind strongly to heparin, apparently due to electrostatic interactions between the negatively charged polysaccharide and a positively charged region of the 8 kDa gelsolin assemblies. Our observations are consistent with a scaffolding mechanism, whereby cross-?-sheet oligomers, upon formation, bind to GAGs, accelerating the fibril extension phase of amyloidogenesis, possibly by concentrating and orienting the oligomers to more efficiently form amyloid fibrils. Notably, heparin decreases the 8 kDa gelsolin concentration necessary for amyloid fibril formation, likely a consequence of fibril stabilization through heparin binding. Since GAG overexpression, which is common in amyloidosis, may represent a strategy to minimize cross-?-sheet oligomer toxicity by transforming them into amyloid fibrils, the mechanism described herein for GAG-mediated acceleration of 8 kDa gelsolin amyloidogenesis provides a starting point for therapeutic strategy development. The addition of GAG mimetics, small molecule sulfonates shown to reduce the amyloid load in animal models of amyloidosis, to a heparin-accelerated 8 kDa gelsolin aggregation reaction neither significantly alters the rate of amyloidogenesis nor prevents oligomers from binding to GAGs, calling into question their commonly accepted mechanism.

Solomon, James P.; Bourgault, Steve; Powers, Evan T.; Kelly, Jeffery W.



Nitrergic Pathway Is the Major Mechanism for the Effect of DA-9701 on the Rat Gastric Fundus Relaxation  

PubMed Central

Background/Aims DA-9701 significantly improved gastric accommodation by increasing the postprandial gastric volume. In this study, we investigated how DA-9701 affects the rat gastric fundus relaxation. Methods Gastric fundus muscle strips (9 longitudinal and 7 circular muscles) were obtained from rats. Electrical field stimulation (EFS) was performed at various frequencies (1, 5, 10 and 20 Hz) and train durations (1, 5, 10 and 20 seconds) to select optimal condition for experiments. Isometric force measurements were performed in response to EFS. Peak and nadir were observed during the first 1 minute after initiation of EFS in control state and after sequential addition of atropine (1 ?M), DA-9701 (0.5, 5, 25 and 50 ?g), N-nitro-L-arginine (L-NNA, 100 ?M), MRS2500 (1 ?M) and tetrodotoxin (TTX, 1 ?M) to the organ bath. Results The optimal frequency and duration of EFS to evoke nerve-mediated relaxation was determined as 5 Hz for 10 seconds. Addition of L-NNA in the presence of atropine and DA-9701 (50 ?g) decreased nadir by inhibiting relaxation from ?0.054 ± 0.021 g to ?0.022 ± 0.015 g (P = 0.026) in longitudinal muscles. However, subsequent application of MRS2500 in the presence of atropine, DA-9701 (50 ?g) and L-NNA did not affect nadir. In circular muscles, subsequent addition of L-NNA and MRS2500 in the presence of atropine and DA-9701 (50 ?g) did not show significant change of nadir. Conclusions Our data suggest that the effect of DA-9701 on the rat gastric fundus relaxation is mainly mediated by nitrergic rather than purinergic pathway.

Min, Yang Won; Ko, Eun-ju; Lee, Ji Yeon; Min, Byung-Hoon; Lee, Jun Haeng; Kim, Jae J; Rhee, Poong-Lyul



Cloning and expression in Escherichia coli of DNA encoding a 60 kDa stress protein of Mycobacterium paratuberculosis, the causative agent of Johne's disease  

Microsoft Academic Search

Polymerase chain reaction (PCR) was used to generate DNA encoding a 60 kDa stress protein of Mycobacterium paratuberculosis using primers complementary to sequences at the 5' and 3' ends of 60 kDa stress protein genes (encoding the '65 kDa antigens') of M. leprae and M. tuberculosis. The predicted PCR product of 1.8 kb contained the entire coding sequence of an

Angela Colston; Ian McConnell; R. Bujdoso



Vaccination of ducks with recombinant outer membrane protein (OmpA) and a 41 kDa partial protein (P45N?) of Riemerella anatipestifer  

Microsoft Academic Search

The generation of protective immunity against Riemerellaanatipestifer infection in ducks were investigated by immunizations with recombinant glutathione sulfatransferase (GST) fusion’s proteins of OmpA, a 42kDa major outer membrane protein, and P45N?, a 41kDa N-terminal fragment of a newly identified 45kDa potential surface protein from R. anatipestifer. The DNA encoding OmpA and P45N? were isolated from R. anatipestifer serotype 15 (field

Bin Huang; Sumathi Subramaniam; Joachim Frey; Hilda Loh; Hai-Meng Tan; Charlene J Fernandez; Jimmy Kwang; Kim-Lee Chua



A geochemical and geophysical approach to derive a conceptual circulation model of CO 2 -rich mineral waters: A case study of Vilarelho da Raia, northern Portugal  

Microsoft Academic Search

.   The Vilarelho da Raia-Chaves region, located in northern Portugal adjacent to the Spanish border, is characterized by both\\u000a hot and cold CO2-rich mineral waters issuing from springs and drilled wells. The present paper updates the conceptual circulation model of\\u000a the Vilarelho da Raia cold CO2-rich mineral waters. Vilarelho da Raia mineral waters, dominated by Na and HCO3 ions, have

J. M. Marques; Monteiro F. Santos; R. Graça; R. Castro; L. Aires-Barros; L. Mendes Victor



Bullous Pemphigoid and Linear IgA Dermatosis Sera Recognize a Similar 120-kDa Keratinocyte Collagenous Glycoprotein with Antigenic Cross-Reactivity to BP180  

Microsoft Academic Search

Circulating IgG from a large subset of bullous pemphigoid (BP) patients reacted on immunoblot with a 120-kDa protein in conditioned keratinocyte culture medium and in keratinocyte cell extracts. A protein with a similar molecular weight was recognized by circulating IgA from a subset of patients with linear IgA dermatosis (LAD). Both affinity-purified 120- kDa-specific BP IgG and 120-kDa-specific LAD IgA

Hendri H. Pas; Guus J. Kloosterhuis; Klaas Heeres; Jan B. Van der Meer; Marcel F. Jonkman



Antibodies elicited by a virosomally formulated Plasmodium falciparum serine repeat antigen-5 derived peptide detect the processed 47 kDa fragment both in sporozoites and merozoites  

Microsoft Academic Search

Serine repeat antigen-5 (SERA5) is a candidate antigen for inclusion into a malaria subunit vaccine. During merozoite release and reinvasion the 120kDa SERA5 precursor protein (P120) is processed, and a complex consisting of an N-terminal 47kDa (P47) and a C-terminal 18kDa (P18) processing product associates with the surface of merozoites. This complex is thought to be involved in merozoite invasion

Shinji L. Okitsu; Francesca Boato; Markus S. Mueller; Dong Bo Li; Denise Vogel; Nicole Westerfeld; Rinaldo Zurbriggen; John A. Robinson; Gerd Pluschke



The effect of DA8159, a novel PDE5 inhibitor, on erectile function in the rat model of hypercholesterolemic erectile dysfunction  

Microsoft Academic Search

This study examined the effects of a new phosphodiesterase type 5 inhibitor, DA-8159, on erectile function associated with hypercholesterolemia. First of all, in order to investigate whether chronic administration of DA-8159 prevents the development of erectile dysfunction associated with hypercholesterolemia, male SD rats were divided into four groups (normal control, hypercholesterolemic control, DA-8159 5 or 20 mg\\/kg\\/day). Over a 5-month

K K Kang; J Y Yu; M Yoo; J W Kwon



Isolation and Sequence of a cDNA Clone Encoding the 31-kDa Subunit of Bovine Kidney Vacuolar H+ATPase  

Microsoft Academic Search

We have isolated a cDNA encoding the 31-kDa subunit of the bovine kidney vacuolar H+-ATPase. The composite sequence contains 1219 base pairs, which includes the entire 678-base-pair coding region. A lysine-rich sequence previously found in the Na+, K+-ATPase alpha subunit and the H+, K+-ATPase was identified in the 31-kDa subunit. An RNA blot and an immunoblot demonstrated variable 31-kDa subunit

Sheldon Hirsch; Arnold Strauss; Khalid Masood; Stephen Lee; Vikas Sukhatme; Stephen Gluck



Immunoblot evaluation of the 100 and 130 kDa antigens in camel hydatid cyst fluid for the serodiagnosis of human cystic echinococcosis in Libya  

Microsoft Academic Search

Two high molecular weight antigens with molecular masses approximately 100 kDa and 130 kDa were identified by immunoblotting camel hydatid cyst fluid, with 94% sensitivity in sera from surgically confirmed Libyan cystic echinococcosis cases. 40% of sera from surgically confirmed alveolar echinococcosis cases cross-reacted with the 100 and 130 kDa antigens, as did 5·3% of sera from human Taenia solium

M. K. Shambesh; P. S. Craig; A. M. Gusbi; E. F. Echtuish; H. Wen



Purification and spectroscopic properties of 124-kDa oat phytochrome.  


A simplified procedure for the isolation and purification of 124-kDa phytochrome from etiolated Avena seedlings has been developed using the method of ammonium sulfate back-extraction. After hydroxyapatite chromatography of seedling tissue extracts, the pooled phytochrome was subjected to ammonium sulfate back-extraction instead of the usual application to an Affi-Gel Blue column. The resulting phytochrome had specific absorbance ratios (SAR = A666/A280) ranging from 0.85 to 0.95. Subsequent Bio-Gel filtration chromatography yielded highly pure 124-kDa phytochrome with SAR values ranging from 0.99 to 1.13. The absorption maxima of 124-kDa phytochrome were at 280, 379, and 666 nm for the red absorbing form of phytochrome (Pr) and at 280, 400 and 730 nm for the far-red absorbing form (Pfr). The A730/A673 ratio in Pfr was found to be 1.5 to 1.6. The mole fraction of Pfr under red light photoequilibrium was 0.88. No dark reversion was detected within 5 h at 3 degrees C. A photoreversible far-uv-circular dichroism was observable with all phytochrome preparations examined. Fluorescence and phosphorescence lifetimes were measured to further characterize the differences between the phytochromes prepared under different conditions. The Trp fluorescence and phosphorescence lifetimes of Pr and Pfr with the chromophore "X", probably polyphenolic in nature, were significantly shorter than those of phytochrome without the contaminant X. The short lifetime of the fluorescence of the Pr chromophore is attributable to X in the former. PMID:3661984

Chai, Y G; Singh, B R; Song, P S; Lee, J; Robinson, G W



Projeto do sistema anti-ressonante da fiação dos transdutores para o detector Mario Schenberg  

NASA Astrophysics Data System (ADS)

O detector de ondas gravitacionais Mario Schenberg está sendo projetado e construído pelo grupo Gráviton. Sua construção está ocorrendo no Laboratório de Estado Sólido e Baixas Temperaturas (LESBT) da Universidade de São Paulo, na cidade de São Paulo. Esse detector possui uma massa ressonante esférica de cobre-alumínio, com 65 cm de diâmetro, pesando aproximadamente 1150 Kg, suspensa por um sistema de isolamento vibracional, que se encontra em fase de testes preliminares. A real eficácia desse sistema, entretanto, só poderá ser comprovada quando o detector estiver aparelhado com, pelo menos, um transdutor eletromecânico de altíssima sensibilidade acoplado à massa ressonante. Neste momento, não só este sistema de isolamento vibracional será posto em teste, como o do projeto da fiação que transporta os sinais de microondas até os transdutores e destes para a pré-amplificação. Apesar dessa fiação ter sido projetada para não apresentar nenhum contato com a superfície esférica da antena, de maneira a não haver nenhuma transmissão de ruído vibracional do laboratório para esta, deve-se minimizar o ruído microfônico produzido nessa fiação por oscilações mecânicas, uma vez que ela não utiliza nenhum sistema de isolamento vibracional. Com o intuito de resolver este problema, projetamos uma estrutura, formada por pequenos cilindros conectados por barras, a qual não terá nenhuma ressonância mecânica na faixa de freqüências de interesse para detecção (3000 - 3400 Hz). Desta forma, as vibrações nessa faixa não serão amplificadas. O projeto foi feito usando iterativamente, de maneira a otimizar os resultados obtidos, o programa de elementos finitos Msc/Nastran. Através de simulações feitas neste programa, determinamos os parâmetros geométricos ideais a serem utilizados, os quais proporcionam a maior região espectral de interesse livre de ressonâncias.

Vieira, S. J.., Jr.; Melo, J. L.



Purification and spectral studies on the Ca2+-binding properties of 67 kDa calcimedin.  

PubMed Central

The 67 kDa calcimedin, isolated by using a phenyl-Sepharose affinity column followed by DEAE-cellulose and gel-filtration chromatographies, was homogeneous by the criterion of SDS/polyacrylamide-gel electrophoresis. In non-SDS gels, the protein moved faster in the presence of EDTA, suggesting that Ca2+ binding affects its mobility in a manner similar to other Ca2+-binding proteins such as calmodulin and S-100 proteins. The 67 kDa protein underwent a conformational change upon binding Ca2+, as revealed by u.v. difference spectroscopy and near-u.v. c.d. measurements. Tryptophan and tyrosine residues were perturbed upon Ca2+ binding, moving to a more non-polar environment in the presence of Ca2+. Upon excitation of the protein at 280 nm, the fluorescence emission maximum was centered around 325 nm, suggesting that the tryptophan residues are located in a fairly hydrophobic region. Ca2+ addition did not induce a significant change in the intrinsic protein fluorescence intensity at 325 nm. Addition of Ca2+ to the 67 kDa protein labelled with 2-p-toluidinylnaphthalene-6-sulphone (TNS) resulted in a 25% increase in fluorescence intensity, accompanied by a blue shift of the emission maximum from 442 to 432 nm. Hence, the probe in the presence of Ca2+ moves to a more non-polar microenvironment, like calmodulin and other Ca2+-binding proteins. Fluorescence titration with Ca2+ using TNS-labelled protein revealed one class of binding site, with a Kd value of 2 x 10(-5) M. Images Fig. 2.

Mani, R S; Kay, C M



Cloning and expression of human 75-kDa inositol polyphosphate-5-phosphatase.  


Inositol polyphosphate-5-phosphatase (5-phosphatase) hydrolyzes inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate and thereby functions as a signal terminating enzyme in cellular calcium ion mobilization. A cDNA encoding human platelet 5-phosphatase has been isolated by screening for beta-galactosidase fusion proteins that bind to inositol 1,3,4,5-tetrakisphosphate. The sensitivity of the screening procedure was enhanced 50- to 100-fold by amplification of "sublibraries" prior to carrying out binding assays. The sequences derived from the "expression clone" were used to screen human erythroleukemia cell line and human megakaryocytic cell line cDNA libraries. We obtained two additional clones which together consist of 2381 base pairs. The amino-terminal amino acid sequence from the 75-kDa 5-phosphatase purified from platelets is identical to amino acids 38-56 predicted from the cDNA. This suggests that the platelet 5-phosphatase is formed by proteolytic processing of a larger precursor. The cDNA predicts that the mature enzyme contains 635 amino acids (Mr 72, 891). Antibodies directed against recombinant TrpE fusion proteins of either an amino-terminal region or a carboxyl-terminal region immunoprecipitate the enzyme activity from a preparation of the 75-kDa form of platelet 5-phosphatase (Type II) but do not precipitate the distinct 47-kDa 5-phosphatase (Type I) also found in platelets. In addition, the recombinant protein expressed in Cos-7 cells has the same 5-phosphatase activity as the platelet 5-phosphatase. PMID:1718960

Ross, T S; Jefferson, A B; Mitchell, C A; Majerus, P W



Diagnostic potential of 16 kDa (HspX, ?-crystalline) antigen for serodiagnosis of tuberculosis  

PubMed Central

Background & objectives: Tuberculosis (TB) is a public health problem worldwide. Rapid and accurate diagnosis of tuberculosis is crucial to facilitate early treatment of infectious cases and to reduce its spread. The present study was aimed to evaluation of 16 kDa antigen as a serodiagnostic tool in pulmonary and extra-pulmonary tuberculosis patients in an effort to improve diagnostic algorithm for tuberculosis. Methods: In this study, 200 serum samples were collected from smear positive and culture confirmed pulmonary tuberculosis patients, 30 tubercular pleural effusions and 21 tubercular meningitis (TBM) patients. Serum samples from 36 healthy, age matched controls (hospital staff), along with 60 patients with non-tubercular respiratory diseases were also collected and evaluated. Humoral response (both IgG and IgA) was looked for 16 kDa antigen using indirect ELISA. Results: Sensitivity of detection in various categories of pulmonary TB patients ranged between 73.8 and 81.2 per cent. While in the extra-pulmonary TB samples the sensitivity was 42.8 per cent (TBM) and 63.3 per cent (tubercular pleural effusion). The test specificity in both the groups was high (94.7%). All of the non-disease controls were negative. Among non-tubercular disease controls, five patients gave a positive humoral response against 16 kDa. Interpretation & conclusions: Serodiagnostic tests for TB have always had drawbacks of suboptimal sensitivity and specificity. The antigen used in this study gave encouraging results in pulmonary TB only, while in extra-pulmonary TB (tubercular meningitis and tubercular pleural effusion), this has shown a limited role in terms of sensitivity. Further work is required to validate its role in serodiagnosis of TB especially extra-pulmonary TB.

Kaushik, Amit; Singh, Urvashi B.; Porwal, Chhavi; Venugopal, Shwetha J.; Mohan, Anant; Krishnan, Anand; Goyal, Vinay; Banavaliker, Jayant N.



Rectifying performance of D-?-A molecules based on cyanovinyl aniline derivatives  

NASA Astrophysics Data System (ADS)

Using the first-principles method, we investigate rectifying performances of D-?-A molecules based on cyanovinyl aniline derivatives. The calculated results show that different functional groups can change the location of molecular orbitals and thus change the rectifying properties of molecules. Interestingly, we find that although the electronic structure for our studied systems is in agreement with that proposed originally by Aviram and Ratner [Chem. Phys. Lett. 29, 277 (1974)], the rectifying direction is opposite from it due to the asymmetric shift of molecular levels under biases of different polarities. Only for model (M4), it shows a forward rectifying performance under larger bias.

Pan, J. B.; Zhang, Z. H.; Deng, X. Q.; Qiu, M.; Guo, C.



Response to the comments on "The formation of Pangea" by D.A. Ruban  

NASA Astrophysics Data System (ADS)

The Variscan structures of the Caucasus region are still quite difficult to decipher, they certainly deserved some in depth investigations in the future. Thus, it is right to question any paleogeographic models proposed in that area, as made by D.A. Ruban. We present here the arguments that we used to decide on the distribution of the terranes in that region. The Transcaucasus massif is regarded as pertaining to the Galatian super-terrane, whereas, the Great Caucasus terrane belongs to the Hanseatic ribbon terrane. The latter was a part of Hunia, detached from Laurussia in the Devonian.

Stampfli, Gérard M.



The strainrange partitioning behavior of an advanced gas turbine disk alloy, AF2-1DA  

NASA Technical Reports Server (NTRS)

The low-cycle, creep-fatigue characteristics of the advanced gas turbine disk alloy, AF2-1DA have been determined at 1400 F and are presented in terms of the method of strainrange partitioning (SRP). The mean stresses which develop in the PC and CP type SRP cycles at the lowest inelastic strainrange were observed to influence the cyclic lives to a greater extent than the creep effects and hence interfered with a conventional interpretation of the results by SRP. A procedure is proposed for dealing with the mean stress effects on life which is compatible with SRP.

Halford, G. R.; Nachtigall, A. J.



p56lck stably associates with a 115 kDa substrate.  


Using antibodies directed against p56lck, we have identified a 115 kDa protein (p115) that is specifically immunoprecipitated with p56lck from whole cell lysates. The p56lck/p115 complex is stable in the presence of nonionic detergents. p115 becomes phosphorylated on tyrosine residues in p56lck immune-complex kinase assays. Treatment of whole cells with 12-O-tetradecanoyl phorbol-13-acetate decreases the subsequent tyrosine phosphorylation of p115 in immune-complex kinase assays. PMID:1632788

Srinivas, P R; Hutchcroft, J E; Aube, B D; Harrison, M L



Utilities for Quantifying Separation in PCA/PLS-DA Scores Plots  

PubMed Central

Metabolic fingerprinting studies rely on interpretations drawn from low-dimensional representations of spectral data generated by methods of multivariate analysis such as PCA and PLS-DA. The growth of metabolic fingerprinting and chemometric analyses involving these low-dimensional scores plots necessitates the use of quantitative statistical measures to describe significant differences between experimental groups. Our updated version of the PCAtoTree software provides methods to reliably visualize and quantify separations in scores plots through dendrograms employing both nonparametric and parametric hypothesis testing to assess node significance, as well as scores plots identifying 95% confidence ellipsoids for all experimental groups.

Worley, Bradley; Halouska, Steven; Powers, Robert



Study of the hadronic interactions of kaons in light nuclei at DA?ne  

NASA Astrophysics Data System (ADS)

The AMADEUS experiment at the Da?ne accelerator of the Frascati National Laboratories (Italy) of INFN, will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, with a complete experimental program for the case of the kaonic clusters. Studying the absorption of antikaon by the nucleus will provide information concerning the KN interaction and the modification of the kaon mass in the nuclear medium. A preliminar study of these kind of hadronic interactions is being done by the AMADEUS collaboration by analyzing the existent KLOE data.

Vazquez Doce, O.



Towards a Standardized Line List for G 191-B2B and other DA Type Objects  

NASA Astrophysics Data System (ADS)

We present a comprehensive analysis of the far UV spectrum of G 191-B2B over the range of 900-1700Å using co-added data from the FUSE and STIS archives. While previous identifications made by Holberg et al. (2003) are reaffirmed in this work, it is found that many previously unidentified lines can now be attributed to Fe, Ni, and a few lighter metals. Future work includes extending this detailed analysis to a wider range of DA objects, in the expectation that a more complete analysis of their atmospheres can be realised.

Preval, S. P.; Barstow, M. A.; Holberg, J. B.; Dickinson, N. J.



Role of the 37 kDa laminin receptor precursor in the life cycle of prions.  


Prions are thought to consist of infectious proteins that cause, in the absence of detectable nucleic acid, a group of fatal neurodegenerative diseases, called transmissible spongiform encephalopathies (TSE). Among these diseases are bovine spongiform encephalopathy (BSE), scrapie of sheep and Creutzfeldt-Jakob disease (CJD) in humans. They occur as sporadic, infectious or genetic disorders and have in common the accumulation of an abnormal, pathogenic isoform of the cellular prion protein PrPc which is converted in a post-translational process into PrPSc concomitant with conformational changes of the protein. During this process PrPc acquires a high beta-sheet content and becomes partially resistant to proteases. The mechanism of this conversion as well as the physiological function of the cellular prion protein PrPc are poorly understood, but studies employing PrP knock-out mice demonstrated that PrPc is required for the development of prion diseases. The involvement of co-factors such as chaperones, receptors or an unknown protein, designated "protein X" in the conversion process are discussed. In a yeast two-hybrid screen we have identified the 37 kDa laminin receptor precursor (LRP) as an interactor of the cellular prion protein and this interaction could be confirmed by co-infection and co-transfection studies in mammalian and insect cells. LRP evolved from the ribosomal protein p40 essential for protein synthesis lacking any laminin binding activity to a cell surface receptor binding laminin, elastin and carbohydrates. The gene encoding 37 kDa LRP/p40 has been identified in a variety of species including the sea urchin Urechis caupo, Chlorohydra viridissima, the archaebacterium Haloarcula marismortui, the yeast Saccharomyces cerevisiae as well as in mammals where it is highly conserved. LRP works as a receptor for alphaviruses and is associated with the metastatic potential of solid tumors where it was first identified. The 37 kDa LRP forms its mature 67 kDa isoform with high laminin binding capacity by an unknown mechanism involving acylation. The multifunctionality of LRP as a ribosomal protein and a cell surface receptor for infectious agents such as viruses and prions might be extended by additional properties. PMID:10188208

Rieger, R; Lasmézas, C I; Weiss, S



Structural health monitoring of the church of Santa Casa da Misericordia of Aveiro using FBG sensors  

NASA Astrophysics Data System (ADS)

This paper presents a structural health monitoring system, based on fibre Bragg gratings, developed for the church of Santa Casa da Misericordia of Aveiro. This system comprises 19 displacement sensors and 5 temperature sensors. All the sensors were custom made according to the monitoring points' characteristics. The results obtained over the first months are presented. The objective of this work is to gather data that will bring a deeper knowledge of how this structure behaves and to help planning the recovering interventions in this historical building.

Lima, H. F.; Vicente, R.; Nogueira, R. N.; Abe, I.; André, P.; Fernandes, C.; Rodrigues, H.; Varum, H.; Kalinowski, H. J.; Costa, A.; Pinto, J. L.



Pharmacological profile of DA-6886, a novel 5-HT4 receptor agonist to accelerate colonic motor activity in mice.  


DA-6886, the gastrointestinal prokinetic benzamide derivative is a novel 5-HT4 receptor agonist being developed for the treatment of constipation-predominant irritable bowel syndrome (IBS-C). The purpose of this study was to characterize in vitro and in vivo pharmacological profile of DA-6886. We used various receptor binding assay, cAMP accumulation assay, organ bath experiment and colonic transit assay in normal and chemically constipated mice. DA-6886 exhibited high affinity and selectivity to human 5-HT4 receptor splice variants, with mean pKi of 7.1, 7.5, 7.9 for the human 5-HT4a, 5-HT4b and 5-HT4d, respectively. By contrast, DA-6886 did not show significant affinity for several receptors including dopamine D2 receptor, other 5-HT receptors except for 5-HT2B receptor (pKi value of 6.2). The affinity for 5-HT4 receptor was translated into functional agonist activity in Cos-7 cells expressing 5-HT4 receptor splice variants. Furthermore, DA-6886 induced relaxation of the rat oesophagus preparation (pEC50 value of 7.4) in a 5-HT4 receptor antagonist-sensitive manner. The evaluation of DA-6886 in CHO cells expressing hERG channels revealed that it inhibited hERG channel current with an pIC50 value of 4.3, indicating that the compound was 1000-fold more selective for the 5-HT4 receptor over hERG channels. In the normal ICR mice, oral administration of DA-6886 (0.4 and 2mg/kg) resulted in marked stimulation of colonic transit. Furthermore, in the loperamide-induced constipation mouse model, 2mg/kg of DA-6886 significantly improved the delay of colonic transit, similar to 10mg/kg of tegaserod. Taken together, DA-6886 is a highly potent and selective 5-HT4 receptor agonist to accelerate colonic transit in mice, which might be therapeutic agent having a favorable safety profile in the treatment of gastrointestinal motor disorders such as IBS-C and chronic constipation. PMID:24769304

Lee, Min Jung; Cho, Kang Hun; Park, Hyun Min; Sung, Hyun Jung; Choi, Sunghak; Im, Weonbin



New paleomagnetic constraints on the extrusion of Indochina: Late Cretaceous results from the Song Da terrane, northern Vietnam  

NASA Astrophysics Data System (ADS)

Samples were collected for paleomagnetic investigations from the Upper Cretaceous Yen Chau Formation in the Song Da terrane (21.7°N, 103.9°E) bounded by the Ailao Shan-Red River fault system and Song Ma fault, in an attempt to examine extrusion tectonics of East Asia. Primary nature of the high-temperature (600-690 °C) component of magnetization from 13 sites is supported by a positive fold test. The tectonic corrected data provides the characteristic Late Cretaceous paleomagnetic direction for the Song Da terrane ( D=6.4°, I=32.0° with ?95=8.5°, N=13 sites), corresponding to a paleopole lying at 82.9°N, 220.7°E ( A95=6.9°). Comparison with coeval paleomagnetic poles for the neighboring tectonic blocks indicates no latitudinal translation of the Son Da terrane with respect to the South China Block, whereas the Shan-Thai Block and the southern part of the Indochina Block revealed a southward displacement of 10.5±9.5° in latitude with respect to the Song Da terrane. We conclude that the southeastern segment of the Ailao Shan-Red River fault system to the east of the Dien Bien Phu fault is not a demarcation of the extruded Indochina Peninsula. The Indochina Block extruded probably along some faults between the Song Da terrane and the Khorat Plateau.

Takemoto, Kazuhiro; Halim, Nadir; Otofuji, Yo-ichiro; Van Tri, Tran; Van De, Le; Hada, Shigeki



Binding of TFIID and MEF2 to the TATA element activates transcription of the Xenopus MyoDa promoter.  

PubMed Central

Members of the MyoD family of helix-loop-helix proteins control expression of the muscle phenotype by regulating the activity of subordinate genes. To investigate processes that control the expression of myogenic factors and regulate the establishment and maintenance of the skeletal muscle phenotype, we have analyzed sequences necessary for transcription of the maternally expressed Xenopus MyoD (XMyoD) gene. A 3.5-kb DNA fragment containing the XMyoDa promoter was expressed in a somite-specific manner in injected frog embryos. The XMyoDa promoter was active in oocytes and cultured muscle cells but not in fibroblasts or nonmuscle cell lines. A 58-bp fragment containing the transcription initiation site, a GC-rich region, and overlapping binding sites for the general transcription factor TFIID and the muscle-specific factor MEF2 was sufficient for muscle-specific transcription. Transcription of the minimal XMyoDa promoter in nonmuscle cells was activated by expression of Xenopus MEF2 (XMEF2) and required binding of both MEF2 and TFIID to the TATA motif. These results demonstrate that the XMyoDa TATA motif is a target for a cell-type-specific regulatory factor and suggests that MEF2 stabilizes and amplifies XMyoDa transcription in mesodermal cells committed to the muscle phenotype. Images

Leibham, D; Wong, M W; Cheng, T C; Schroeder, S; Weil, P A; Olson, E N; Perry, M



Agarose and Polyacrylamide Gel Electrophoresis Methods for Molecular Mass Analysis of 5-500 kDa Hyaluronan  

PubMed Central

Agarose and polyacrylamide gel electrophoresis systems for the molecular mass-dependent separation of hyaluronan (HA) in the size range of approximately 5–500 kDa have been investigated. For agarose-based systems, the suitability of different agarose types, agarose concentrations, and buffers systems were determined. Using chemoenzymatically synthesized HA standards of low polydispersity, the molecular mass range was determined for each gel composition, over which the relationship between HA mobility and logarithm of the molecular mass was linear. Excellent linear calibration was obtained for HA molecular mass as low as approximately 9 kDa in agarose gels. For higher resolution separation, and for extension to molecular masses as low as approximately 5 kDa, gradient polyacrylamide gels were superior. Densitometric scanning of stained gels allowed analysis of the range of molecular masses present in a sample, and calculation of weight-average and number-average values. The methods were validated for polydisperse HA samples with viscosity-average molecular masses of 112, 59, 37, and 22 kDa, at sample loads of 0.5 µg (for polyacrylamide) to 2.5 µg (for agarose). Use of the methods for electrophoretic mobility shift assays was demonstrated for binding of the HA-binding region of aggrecan (recombinant human aggrecan G1-IGD-G2 domains) to a 150 kDa HA standard.

Bhilocha, Shardul; Amin, Ripal; Pandya, Monika; Yuan, Han; Tank, Mihir; LoBello, Jaclyn; Shytuhina, Anastasia; Wang, Wenlan; Wisniewski, Hans-Georg; de la Motte, Carol; Cowman, Mary K.



Pressure Equilibrium and Jump Study on Unfolding of 23-kDa Protein from Spinach Photosystem II  

PubMed Central

Pressure-induced unfolding of 23-kDa protein from spinach photosystem II has been systematically investigated at various experimental conditions. Thermodynamic equilibrium studies indicate that the protein is very sensitive to pressure. At 20°C and pH 5.5, 23-kDa protein shows a reversible two-state unfolding transition under pressure with a midpoint near 160 MPa, which is much lower than most natural proteins studied to date. The free energy (?Gu) and volume change (?Vu) for the unfolding are 5.9 kcal/mol and ?160 ml/mol, respectively. It was found that NaCl and sucrose significantly stabilize the protein from unfolding and the stabilization is associated not only with an increase in ?Gu but also with a decrease in ?Vu. The pressure-jump studies of 23-kDa protein reveal a negative activation volume for unfolding (?66.2 ml/mol) and a positive activation volume for refolding (84.1 ml/mol), indicating that, in terms of system volume, the protein transition state lies between the folded and unfolded states. Examination of the temperature effect on the unfolding kinetics indicates that the thermal expansibility of the transition state and the unfolded state of 23-kDa protein are closer to each other and they are larger than that of the native state. The diverse pressure-refolding pathways of 23-kDa protein in some conditions were revealed in pressure-jump kinetics.

Tan, Cui-Yan; Xu, Chun-He; Wong, Jun; Shen, Jian-Ren; Sakuma, Shinsuke; Yamamoto, Yasusi; Lange, Reinhard; Balny, Claude; Ruan, Kang-Cheng



Evidence that the 59-kDa protein synthesis initiation factor from wheat germ is functionally similar to the 80-kDa initiation factor 4B from mammalian cells.  


The results of this investigation show that the 59-kDa protein synthesis initiation factor from wheat germ, designated eukaryotic initiation factor (eIF)-4G by Browning et al. (Browning, K.S., Maia, D.M., Lax, S.R., and Ravel, J.M. (1987) J. Biol. Chem. 262, 539-541), cross-links to the 5'-terminal cap of oxidized mRNA in the presence of eIF-4A, eIF-4F, and ATP, stimulates the RNA-dependent ATPase activities of eIF-4A and a mixture of eIF-4A and eIF-4F, and stimulates the unwinding activities of eIF-4A, eIF-4F, and a mixture of eIF-4A and eIF-4F. These findings strongly suggest that the 59-kDa factor from wheat germ is the functional equivalent of the 80-kDa protein synthesis initiation factor, eIF-4B, from mammalian cells. Recent reports indicate that the wheat germ initiation factor which contains two subunits of 80 and 28 kDa and which was given the designation "eIF-4B" by Lax et al. (Lax, S.R., Lauer, S.J., Browning, K. S., and Ravel, J.M. (1986) Methods Enzymol. 118, 109-128) is an isozyme form of eIF-4F and not the functional equivalent of mammalian eIF-4B. On the basis of functional characteristics we propose that the designation for the wheat germ factor containing the 80- and 28-kDa polypeptides be changed from eIF-4B to eIF-(iso)4F and the designation for the 59-kDa factor be changed from eIF-4G to eIF-4B. PMID:2722784

Browning, K S; Fletcher, L; Lax, S R; Ravel, J M



Project Da Vinci: a study of long-range air pollution using a balloon-borne Lagrangian measurement platform. Volume 2. Reports of participants in Da Vinci 1. Final report  

SciTech Connect

Understanding long-range air pollution is becoming increasingly important for the development of cost-effective air-quality control strategies. Project Da Vinci was the beginning of a continuing effort at Sandia to address the need to study long-range air pollution. It consisted of a series of three instrumented, manned balloon flights to study the lower atmosphere in a Lagrangian frame of reference. These experiments gathered information on gases and aerosols, radiation, electrical fields, atmospheric structure, turbulence, diffusion, mesoscale motions, and their interrelationship. Volume II is a collection of the reports from researchers involved in Da Vinci I.

Zak, B.D. (ed.)