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Sample records for santo perspectivas da

  1. Vin Santo.

    PubMed

    Domizio, Paola; Lencioni, Livio

    2011-01-01

    Vin Santo is the typical dessert wine produced in Tuscany, in particular, and in other areas of central and northern Italy, as well as on the Greek island of Santorini. It remains one of the most important "meditation wines", whose glamour starts with its ancient and mysterious origin. Italy is the country with the World's greatest tradition for sweet wine production. In this contest most of the steps in the Vin Santo making process are still linked to old local traditions. This chapter provides an overview of the different styles of Vin Santo produced in Italy together with its marketing. The main factors affecting grapes drying, alcoholic fermentation, maturation in barrels, are also described. So far there have been few scientific studies that have focused on Vin Santo. However, the increasing demand registered in the last few years for this kind of wine, is now greatly stimulating the research aimed to better manage its peculiar production process. PMID:21867892

  2. The Response of Sediments and Dissolved Organic Matter to Rapid Rainfall in the Santa Maria da Vitoria Watershed, Espírito Santo, BR

    NASA Astrophysics Data System (ADS)

    Ward, N. D.; Firme de Almeida, L.; Dias, G.; Gould, R.; Tan, A.; Bianchi, T. S.; Krusche, A. V.; Keil, R. G.; Richey, J. E.

    2015-12-01

    The Santa Maria da Vitória River supplies over 30% of the water for the greater Vitória, Espírito Santo, BR metropolitan area, which has a population of roughly 1.6 million people. The availability of clean freshwater is severely limited during periods of heavy rainfall because water sanitation facilities are "clogged" by high sediment discharge. The headwaters of the Santa Maria da Vitória River are characterized by relatively pristine forested environments, transitioning into primarily agricultural and rural land uses, and finally reaching the large urban center of Vitória near its marine receiving waters. The discharge of suspended sediments and dissolved organic matter (DOM) was examined at a 3 hour frequency during heavy storm flows from October 2013 to May 2015 in the Santa Maria da Vitória River main channel and a small tributary, the Mangaraí River. Bulk isotopic analyses were used to determine potential sediment sources and whether specific landscape/land use features were functionalized during periods of high runoff. Likewise, time of flight mass spectrometry (GC-ToF-MS) was used to identify a broad suite of DOM compounds that responded positively with river discharge in an effort to determine the influence of land use on the delivery of dissolved components to the river. For example, the abundance of compounds related to specific agricultural settings increased during storm flow along with anthropogenic DOM sources such as plasticizer and pesticide-derived compounds. Suspended sediment concentrations increased by as much as 70 times during peak river discharge relative to base flow several days earlier with similar increases in particulate organic carbon and nitrogen observed. Results from this study and previous field measurements were integrated into a coupled hydrology-sediment transport model, DHSVM, as part of a dynamic information framework with the goal of predicting water/sediment discharge to inform management and policy sectors of the

  3. Interview with Boaventura de Sousa Santos

    ERIC Educational Resources Information Center

    Dalea, Roger; Robertson, Susan

    2004-01-01

    Boaventura de Sousa Santos is Professor of Sociology, School of Economics, University of Coimbra and Distinguished Legal Scholar, Law School, University of Wisconsin-Madison. He is also director of the Center for Social Studies at the University of Coimbra. Santos is one of the outstanding theorists whose work is engaged with pressing social and…

  4. [Haitian migration to Santo Domingo].

    PubMed

    Latortue, P R

    1985-01-01

    This work examines the history of Haitian migration to the Dominican Republic, the central role of Haitian migration in Dominican society, working conditions of Haitian migrants in the Dominican Republic, and the relationship of the migration to economic development on the island of Hispaniola. Lack of data, the difficulty of measuring illegal movement, and the problem of defining Haitians in Santo Domingo have impeded understanding of migration to the Dominican Republic. It is believed by many authorities that Haitian migration to Santo Domingo is considerable and perhaps exceeds that to the US. Haitian migration to the Dominican Republic began after 1915 with the fall of the Haitian president, a worsening of economic conditions partly caused by stagnation in the agricultural sector, and the newly dominant role of the US in Haitian economic affairs. The Great Depression of the 1930s was a direct antecedent of the massacre of Haitians by Dominican police in which some 30 thousand persons were killed; the economic recession of the early 1980s has also caused an outburst of antiHaitian feeling in the Dominican Republic although 80% of laborers in the sugar industry are Haitians. Sugar is extremely important to the Dominican economy: in 1974, sugar covered 12% of cultivated land, produced 40% of foreign exchange earnings, and was responsable for 21% of taxable income. Dominicans however refuse to work in sugar plantations under the current technological. conditions and wage system. Although the government periodically demands the Dominicanization of the sugar work force, no such changes have been made. Sugar will probably continue to play a decisive role in the generation of foreign exchange despite introduction of more technologically advanced sectors which benefit from better prices in the international market. Possibilities of mechanizing sugar production in the Dominican Republic appear remote, and failure to modernize an important sector of the economy has

  5. Teaching and Popularization of Astronomy in Latin America by the Liada Perspective. (Spanish Title: Enseñanza y Divulgación de la Astronomía en la América Latina en la Perspectiva de la Liada.) Ensino e Divulgação da Astronomia na América Latina na Perspectiva da Liada

    NASA Astrophysics Data System (ADS)

    Bretones, Paulo Sergio

    2008-12-01

    The goal of this work is to present an analysis of the developed activities of the Teaching and Popularization Section (SEDA) of the Liga Ibero-Americana de Astronomía (LIADA). After a history of the LIADA, are presented the projects of the section that have the support of 16 coordinators from most Latin-American countries. The projects that aim to attract the attention of the general public, teachers and students to encourage the observation and send reports to be posted at the Internet page are presented. More specifically, the projects and reports related with eclipses ocurriesd since the year 2000. Using the available files on the page of the section, an analysis and discussion about their importance for scientific education is done. It is presented a data form as a suggestion for reports buy individuais or institutions and the importance of the systematization of experiences to give more visibility and changes of informations in the area. It is concluded with an assessment of the projects, their potential and limitations, as well as suggestions of future projects looking for more interaction between the Latin American countries and making the Section available to this goal.

    Esto trabajo visa divulgar y analizar las actividades de La Sección de Enseñanza y Divulgación de la Astronomía (SEDA) de la Liga Iberoamericana de Astronomía (LIADA). Después de un histórico da la LIADA, son presentados los diversos proyectos de la Sección que cuenta con la colaboración de los coordinadores locales en la mayoría de los países de la América Latina. Son presentados los proyectos que visan chamar la atención de público en general, estudiantes y profesores para la observación del cielo e posterior envío de los relatos para colocación en la página de La Sección en la Internet. Más específicamente son analizados los proyectos y relatos relacionados con los eclipses ocurridos desde el año 2000. Utilizando-se los ficheros

  6. UNDERSTANDING--SANTO DOMINGO'S RX FOR THE "CULTURAL SHOCK."

    ERIC Educational Resources Information Center

    LOPEZ, REBECCA

    THE SANTO DOMINGO, NEW MEXICO, PUBLIC ELEMENTARY SCHOOL ENROLLS 720 INDIAN PUPILS IN GRADES 1 THROUGH 8 AND UTILIZES 30 TEACHERS IN ITS INSTRUCTIONAL PROGRAM. ALTHOUGH THE PUPILS COME FROM A CULTURE WHICH IS UNFAMILIAR TO MOST OF THEIR TEACHERS, A PRIMARY OBJECTIVE OF THE SCHOOL IS THAT THE LIFE, CUSTOMS, BELIEFS, AND CULTURE OF THE INDIAN…

  7. Diaspididae (Hemiptera: Coccoidea) of Espírito Santo, Brazil

    PubMed Central

    Culik, Mark P.; Martins, David S.; Ventura, José A.; Wolff, Vera S.

    2008-01-01

    Twenty-seven species of armored scale insects (Hemiptera: Diaspididae) are newly recorded from Espírito Santo, Brazil, and information on the host plants and geographic distribution of the 31 species of Diaspididae that have been identified in the State is provided. New plant host records are reported for 11 of the diaspidid species studied and results are discussed with respect to development of agriculture in this and similar areas with objectives of modernization and diversification. PMID:20337558

  8. Continuous Mantle Exhumation at the Outer Continental Margin of the Santos, Campos and Espírito Santo Basins, Brazil

    NASA Astrophysics Data System (ADS)

    Zalan, P. V.; Severino, M. G.; Rigoti, C. A.; Magnavita, L. P.; Oliveira, J. B.; Viana, A. R.

    2011-12-01

    The interpretation of 12,000 km of very deep (PSTM to 16 sec., PSDM to 25 km) 2D seismic sections, coupled with gravimetric and magnetometric modeling line-by-line, and the integration of the results with the regional data bank of Petrobras, all together viewed in terms of the recent tectonic models developed for the rupturing and separation of mega-plates, led to a regional (500,000 km2), first-time ever, 3D-view of the deep structure underlying the prolific sedimentary basins of Santos, Campos and Espírito Santo in southeastern Brazil. The three basins are situated onto a continental margin that narrows gradually, from south to north, from a very wide (Santos), through an intermediate (Campos), and then to a narrow (Espírito Santo) passive margin. The seismic sections shows very well the dual rheological behavior of the continental crust, consisting of a deeper and plastic lower crust (with numerous short and strong reflections that display sub-horizontal ductile flow) and a shallower and brittle upper crust (represented by a mostly transparent and faulted seismic facies topped by the sedimentary sections of the rift and thermal subsidence phases). The crustal structure of the Santos Basin shows a zonation from west to east of alternating bands of NE-SW-trending thin (plastic basement terrains) and thick (resistant basement terrains) stretched continental crust. In vertical section this zonation is displayed as a series of necking zones, leading to a highly irregular, low to moderate crustal taper. Such zonation is less developed in the Campos Basin, where the crustal taper is moderate and regular, and practically non-existent in the Espírito Santo Basin, where the crustal taper is high. The most outstanding crustal feature shared in common by the three basins is the exhumation of mantle between the tip of the hyper-extended continental crust and the tabular-shaped oceanic crust. Although the crustal taper varies significantly from basin to basin their

  9. Other Worlds Are Possible: An Interview with Boaventura de Sousa Santos

    ERIC Educational Resources Information Center

    Phipps, Alison

    2007-01-01

    This article presents an interview with Professor Boaventura de Sousa Santos. Santos is a professor of Sociology at the University of Coimbra, where he is the director of the internationally renowned Centro de Estudos Sociais. He offers a rich vein of theoretical reflection and translation for language(s) and intercultural communication.

  10. The Role of Rhetoric in the Judicial Opinion: The Rhetoric of Boaventura De Sousa Santos.

    ERIC Educational Resources Information Center

    Anapol, Malthon M.

    Boaventura De Sousa Santos, a Portuguese law professor, appears to be the first legal scholar and theorist who works with rhetorical theories and who is accepted by the legal profession. Santos's major works have centered on the legal system of Pasargada, a Brazilian favela (squatter village) whose residents try to settle disputes outside the…

  11. Forbidden therapies: Santo Daime, ayahuasca, and the prohibition of entheogens in Western society.

    PubMed

    Blainey, Marc G

    2015-02-01

    Santo Daime, a Brazilian religion organized around a potent psychoactive beverage called ayahuasca, is now being practiced across Europe and North America. Deeming ayahuasca a dangerous "hallucinogen," most Western governments prosecute people who participate in Santo Daime. On the contrary, members of Santo Daime (called "daimistas") consider ayahuasca a medicinal sacrament (or "entheogen"). Empirical studies corroborate daimistas' claim that entheogens are benign and can be beneficial when employed in controlled contexts. Following from anthropology's goal of rendering different cultural logics as mutually explicable, this article intercedes in a misunderstanding between policies of prohibition and an emergent subculture of entheogenic therapy. PMID:24477460

  12. HIV infection and risk behaviors among male port workers in Santos, Brazil.

    PubMed Central

    Larcerda, R; Stall, R; Gravato, N; Tellini, R; Hudes, E S; Hearst, N

    1996-01-01

    OBJECTIVES. This paper measured the extent to which human immunodeficiency virus (HIV) infection has spread among the male working-class population of Santos, Brazil. METHODS. Questionnaires on risk behaviors and blood tests were administered to a random sample (n = 395) of male port workers employed by the Santos Port Authority. RESULTS. Although the rate of HIV infection among these men- the working-class male population of Santos-remains low (1.1%), self-reported behavioral risks for HIV infection are common. CONCLUSIONS. There is still time to prevent a widespread outbreak of HIV infection among the hetero-sexual population of Santos and of the transportation corridors emanating from that city. PMID:8712280

  13. Palaeomagnetic Investigations on Santo Antao, Cape Verde Islands

    NASA Astrophysics Data System (ADS)

    Knudsen, M. F.; Abrahamsen, N.

    2001-12-01

    Samples from five hitherto fairly unexplored young volcanic islands in the Cape Verde Archipelago have been collected for palaeomagnetic studies. Magnetic results from lava flows in 5 profiles located on the island Santo Antão are reported. The Cape Verde Rise situated over the Mesozoic marine anomalies M2-M16, is the result of a slow eastward movement of the Atlantic oceanic lithosphere relative to a hotspot commencing around 20 Ma ago. The uplifted segment of deformed Jurassic oceanic crust seems to be unrelated to the bulk of the igneous activity, which took place during the Neogene. The oldest outcrops encountered on Santo Antão are deeply weathered basalts with an age of 7.57 +/- 0.56 Ma, whereas the investigated profiles cover a time interval from 2.67 +/- 0.05 Ma to 0.38 +/- 0.05 Ma. Investigation of some basic mineral magnetic properties reveal titanomagnetite composition as a general feature, and a pseudo-single-domain behaviour of all lava flows subjected to hysteresis-experiments are found. Flows of normal polarity characterise the Tarrafal Profile, being confined to the Brunhes Chron by 40Ar/39Ar-datings. Two events of reverse polarity are recorded in the stratigraphic column and appear to represent the Big Lost and Emperor Subchrons. The minimum eruption frequency is estimated to be one flow every 2.5 +/- 2.2 Ka. The Agua Nova Profile is likewise dominated by flows of normal polarity belonging to the Brunhes Chron and further contains a few events of reverse polarity. The relatively complex stratigraphy of accessible flows encountered in the Ribeira Grande Area makes the correlation with the global polarity time-scale tentative. The Escabecada Profile records the transition between the lower part of Matuyama and the upper part of the Gauss Chron, and a minimum eruption frequency is estimated to be one flow every 10 +/- 7.1 Ka for the lower part of this profile. The Chã de Morte Profile consists merely of reverse-polarity lava flows belonging to the

  14. Bathymetry, Chirp and Deep Crustal Structure of the Santos Basin SÃO Paulo Ridge Complex (sbspr)

    NASA Astrophysics Data System (ADS)

    Aslanian, D.; Klingelhoefer, F.; Moulin, M.; Schnurle, P.; Rabineau, M.; Afilhado, A.; Roest, W. R.; Feld, A.; Evain, M.; Rochat, A.; Rousic, D.; Rigoti, C. A.; Capechi, E.; Bochenek, G.; Viana, A. R.; Magnavita, L. P.; Szatmari, P.; Neto, M.; Soares, J. P.; Fuck, R. A.; Paula Ribas, M.; De Lima, M.; Corela, C.; Duarte, J.; Matias, L. M.; OBS Team of Sanba Cruise

    2011-12-01

    The SanBa (Santos Basin- Seismic Research experiment) research experiment is a joint project of the Department of Marine Geosciences (IFREMER: Institut Français de Recherche pour l'Exploitation de la MER, France), the Laboratory of "Oceanic Domain" (Institut Universitaire et Européen de la Mer, France), the Faculdade de Ciências da Universidade de Lisboa (Lattex and CGUL, Portugal), the Universidade de Brasilia (Brazil) and PETROBRAS. Its aim is to test hypotheses that have been proposed such as the existence of failed rift and a micro-block (Moulin et al., GSL submitted) or the presence of exhumed mantle on its south-eastern part (Zalan et al., AAPG 2009). Six wide-angle seismic data were acquired together with coincident deep frequency reflection seismic data during the SanBa cruise in Dec 2010 - Jan. 2011 (total > 850 Nm). Chirp and Bathymetry were also acquired during the cruise. The preliminary results suggest a very thin crust (< 5km) in the center and in the south-eastern part of the SBSPR. Both refraction and reflection data present a clear signal of the Moho in the distalmost part of the study area, which seems to preclude the exhumed mantle hypothesis."

  15. New stable isotope results from a 173-year coral from Espiritu Santo, Vanuatu

    NASA Astrophysics Data System (ADS)

    Quinn, Terrence M.; Crowley, Thomas J.; Taylor, Frederick W.

    Long coral records provide valuable information on pentadal-decadal scale climate variability. Previously we have reported on a preliminary analysis of a 173-year record (1806-1978) from the island of Espiritu Santo (Republic of Vanuatu). Although this record contained information in the pentadal-decadal band, low sampling resolution raised questions about the validity of the results. Herein we report new data from the Santo record, in which the sampling resolution has been doubled and the validation database expanded. Variations in coral δ18O at Santo record the combined effects of variations in SST and rainfall-induced salinity changes. The most prominent spectral peaks in the time series are at the annual cycle and 14-15 and 7.4 years. The robust occurrence of the 14-15 year peak provides additional support to the importance of this oscillation as a cause of decadal-scale climate variability. Overall, our new results provide greater credibility to the conclusions raised in the earlier study and indicate that the Santo record can more confidently be incorporated into the still-small network of multicentury coral records of climate change.

  16. Laurie R. Santos: Award for Distinguished Scientific Early Career Contributions to Psychology

    ERIC Educational Resources Information Center

    American Psychologist, 2012

    2012-01-01

    Presents a short biography of one of the winners of the American Psychological Association's Award for Distinguished Scientific Early Career Contributions to Psychology. The 2012 winner is Laurie R. Santos for creative and insightful investigations of cognition across a broad range of species and psychological domains, illuminating cognitive…

  17. Male-specific contributions to the Brazilian population of Espirito Santo.

    PubMed

    de F Figueiredo, Raquel; Ambrosio, Isabela B; Braganholi, Danilo F; Chemale, Gustavo; Martins, Joyce A; Gomes, Veronica; Gusmão, Leonor; Cicarelli, Regina M B

    2016-05-01

    Y chromosome markers have been widely studied due to their various applications in the fields of forensic and evolutionary genetics. In this study, 35 Y-SNPs and 17 Y-STRs were genotyped in 253 males from the State of Espirito Santo, Brazil. A total of 18 haplogroups and 243 haplotypes were detected; the haplogroup and haplotype diversities were 0.7794 and 0.9997, respectively. Genetic distance analysis using the Y-STR data showed no statistically significant differences between Espirito Santo and other admixed populations from Brazil. The classification of paternal lineages based on haplogroups showed a predominant European contribution (85.88 %), followed by African (11.37 %) and Amerindian (2.75 %) contributions. PMID:26076592

  18. The Oldest Case of Decapitation in the New World (Lapa do Santo, East-Central Brazil).

    PubMed

    Strauss, André; Oliveira, Rodrigo Elias; Bernardo, Danilo V; Salazar-García, Domingo C; Talamo, Sahra; Jaouen, Klervia; Hubbe, Mark; Black, Sue; Wilkinson, Caroline; Richards, Michael Phillip; Araujo, Astolfo G M; Kipnis, Renato; Neves, Walter Alves

    2015-01-01

    We present here evidence for an early Holocene case of decapitation in the New World (Burial 26), found in the rock shelter of Lapa do Santo in 2007. Lapa do Santo is an archaeological site located in the Lagoa Santa karst in east-central Brazil with evidence of human occupation dating as far back as 11.7-12.7 cal kyBP (95.4% interval). An ultra-filtered AMS age determination on a fragment of the sphenoid provided an age range of 9.1-9.4 cal kyBP (95.4% interval) for Burial 26. The interment was composed of an articulated cranium, mandible and first six cervical vertebrae. Cut marks with a v-shaped profile were observed in the mandible and sixth cervical vertebra. The right hand was amputated and laid over the left side of the face with distal phalanges pointing to the chin and the left hand was amputated and laid over the right side of the face with distal phalanges pointing to the forehead. Strontium analysis comparing Burial 26's isotopic signature to other specimens from Lapa do Santo suggests this was a local member of the group. Therefore, we suggest a ritualized decapitation instead of trophy-taking, testifying for the sophistication of mortuary rituals among hunter-gatherers in the Americas during the early Archaic period. In the apparent absence of wealth goods or elaborated architecture, Lapa do Santo's inhabitants seemed to use the human body to express their cosmological principles regarding death. PMID:26397983

  19. Complex intrasalt deformation in the Santos Basin, offshore Brazil: the role of density inversion

    NASA Astrophysics Data System (ADS)

    Dooley, Tim; Jackson, Chris; Jackson, Martin; Hudec, Mike; Rodriguez, Clara

    2016-04-01

    Understanding intrasalt structure may elucidate the fundamental kinematics and, ultimately, the mechanics of diapir growth. However, there have been relatively few studies of the internal structure of salt diapirs outside the mining industry because their cores are only partly exposed in the field and poorly imaged in seismic reflection data. 3D seismic reflection from the Santos Basin, offshore Brazil reveal enigmatic allochthonous salt sheets of older evaporites emplaced above an overlying stratified evaporite sequence. Seismic-stratigraphic observations form the basis for a kinematic model invoking: (i) initial inward flow and thickening of the lower mobile salt within the rising wall, and arching of stratified overburden; (ii) breaching of the overburden, ascent of mobile lower evaporites along single or multiple feeders, and emplacement of upper-wall sheets or canopies; and (iii) later diapir squeezing due to regional shortening. We designed and ran physical models to explain how and why these structures occur, and to provide a mechanical basis for our kinematic model. Our first two models simulated salt having uniform internal density, with walls growing by (i) initially symmetric differential loading; and (ii) initially symmetric differential loading plus shortening. These models reproduced anticlines and injection folds seen in the simpler deformed walls in the Santos Basin. However, neither model reproduced the most complex structures (e.g. allochthonous intrusions, steep feeders, recumbent synclines) seen within the Santos evaporites. Thus, we argue differential loading and shortening alone are insufficient to generate these complex structures. In our third model, a less-dense lower evaporite was overlain by denser upper evaporites, similar to the density structure found in Santos Basin wellbores. In this model the wall rose solely by differential loading, with the lower mobile unit breaching the overlying stratified evaporites to form vertical diapirs

  20. The Oldest Case of Decapitation in the New World (Lapa do Santo, East-Central Brazil)

    PubMed Central

    Strauss, André; Oliveira, Rodrigo Elias; Bernardo, Danilo V.; Salazar-García, Domingo C.; Talamo, Sahra; Jaouen, Klervia; Hubbe, Mark; Black, Sue; Wilkinson, Caroline; Richards, Michael Phillip; Araujo, Astolfo G. M.; Kipnis, Renato; Neves, Walter Alves

    2015-01-01

    We present here evidence for an early Holocene case of decapitation in the New World (Burial 26), found in the rock shelter of Lapa do Santo in 2007. Lapa do Santo is an archaeological site located in the Lagoa Santa karst in east-central Brazil with evidence of human occupation dating as far back as 11.7–12.7 cal kyBP (95.4% interval). An ultra-filtered AMS age determination on a fragment of the sphenoid provided an age range of 9.1–9.4 cal kyBP (95.4% interval) for Burial 26. The interment was composed of an articulated cranium, mandible and first six cervical vertebrae. Cut marks with a v-shaped profile were observed in the mandible and sixth cervical vertebra. The right hand was amputated and laid over the left side of the face with distal phalanges pointing to the chin and the left hand was amputated and laid over the right side of the face with distal phalanges pointing to the forehead. Strontium analysis comparing Burial 26’s isotopic signature to other specimens from Lapa do Santo suggests this was a local member of the group. Therefore, we suggest a ritualized decapitation instead of trophy-taking, testifying for the sophistication of mortuary rituals among hunter-gatherers in the Americas during the early Archaic period. In the apparent absence of wealth goods or elaborated architecture, Lapa do Santo’s inhabitants seemed to use the human body to express their cosmological principles regarding death. PMID:26397983

  1. Gravity and Seismic Investigations of the Santo Domingo Basin, Rio Grande Rift, New Mexico

    NASA Astrophysics Data System (ADS)

    Braile, L. W.; Ferguson, J. F.; Boucher, C.; Novitsky, C. G.; O'Shea, P. M.; Daves, J.; Marzen, R.; Mendoza, K.; Rasmussen, T.; Wei, W.; Baldridge, W. S.; Biehler, S.; Claytor, J. M.; Bischoff, S. H.; Ranasinghe, N. R.; Corredor, A.

    2014-12-01

    The SAGE (Summer of Applied Geophysical Experience) program collected new gravity, seismic, electromagnetic and down-hole temperature data in 2014 in the Santo Domingo Basin and adjacent areas of the Rio Grande Rift (RGR) area of northern New Mexico. The SAGE 2014 data collection was part of an integrated geophysical study of the area initiated in 2010 and adds data and interpretations to a W to E transect of the RGR. The transect includes previous SAGE seismic refraction and CMP reflection profiles recorded in 2010 and 2011, some industry seismic reflection data, and detailed gravity observations. Seismic data consisted of a 4.8 km NW to SE profile (120 three-component stations in four overlapping deployments, 20 m station spacing, using a Vibroseis source - 20 m spacing for reflection VPs; 800 m spacing for refraction VPs) along the Borrego Canyon road with both refraction and CMP reflection coverage. About 50,000 seismograms were recorded. The surface conditions (dry unconsolidated sediments) increased surface wave energy and limited the signal-to-noise level of reflection arrivals although some wide-angle reflections with two-way times as great as 1.8 s were visible. The refraction data were modeled with first arrival travel time methods and mainly helped identify the velocity and minimum thickness of the Tertiary Santa Fe group sedimentary rocks in the Santo Domingo Basin. Interpretation of the seismic and gravity data along the transect was aided by refraction velocities, the existence of a nearby regional seismic reflection profile from industry, and lithologies and well-logs from a deep well. Gravity modeling, with significant control on depths of interfaces and densities from the seismic and drill hole data, indicates that the Santo Domingo sedimentary basin has a total depth of about 6 km.

  2. Sampling the Cape Verde Mantle Plume: Evolution of Santo Antão, Cape Verde Islands

    NASA Astrophysics Data System (ADS)

    Holm, P. M.; Wilson, J. R.; Christensen, B. P.; Hansen, L.; Hansen, S. L.; Hein, K. M.; Mortensen, A. K.; Pedersen, R.; Plesner, S.; Runge, M. K.

    2003-12-01

    The 7.5 - 0.1 Ma old volcanics of the northwesternmost Cape Verde Island of Santo Antão show a change from early incompatible element enriched basanite-phonolite series to more enriched nephelinite/melilite nephelinite-phonolite series volcanics all of HIMU OIB type. Mantle melts were derived by 1-4 % melting and had around 12 wt.% MgO. Olivine Fo88-91 is found in many primitive volcanics. Incompatible element modelling shows that the geochemical change of the composition of the primary magmas requires source enrichment by silicate melts of mainly two compositional types. One of these is MORB. Isotopically the > 2 Ma Old Volcanics group can largely be explained by mixing of two components both with relatively radiogenic Sr and unradiogenic Nd of which one is a young HIMU type source (Δ 8/4 ˜ 0 and Δ 7/4 ˜ -5). The period 2 - 0.7 Ma saw two component mixing of two other end members of which one is a young HIMU source with less radiogenic Sr, more radiogenic Nd, Δ 8/4 ˜ -38 and Δ 7/4 ˜ -5, which isotopically is identical to an end member of carbonatites from the neighbouring island of São Vicente and the southern island Santiago. The youngest volcanics show stronger source enrichment, the most silica undersaturated magmas and an old HIMU-type component (Δ 7/4 > 2). The characteristic EM1-type enrichment of the southern Cape Verde Island is not detected on Santo Antão. We argue that the main components of Santo Antão volcanism are plume derived and reflect vertical variation in composition of rising plume material. The inter island variation of the Cape Verdes may reflect a lateral variation of the plume or lithosphere derived components in the southern island volcanics

  3. Cytherellid species (Ostracoda) and their significance to the Late Quaternary events in the Santos Basin, Brazil

    USGS Publications Warehouse

    Bergue, C.T.; Coimbra, J.C.; Cronin, T. M.

    2007-01-01

    Four autochthonous cytherellid species (Cytherella serratula (BRADY, 1880), C. hermargentina WHATLEY et al. 1998, C. pleistocenica sp. nov. and C. santosensis sp. nov.) have been identified from two offshore cores (44 samples) within the Santos Basin. The distribution of these ostracodes is controlled by local hydrological conditions such as the temperature and, possibly, the oxygen minimum zone (OMZ). However, these factors cannot explain completely the species occurrence in the analysed cores, and relative sea level changes and productivity variation driven by climatic changes are proposed as additional explanations for the faunal distribution pattern. ?? E. Schweizerbart'sche Verlagsbuchhandlung (Na??gele u. Obermiller), 2007.

  4. Outbreak of Hantavirus Pulmonary Syndrome, Los Santos, Panama, 1999–2000

    PubMed Central

    Bayard, Vicente; Barria, Eduardo O.; Ruedas, Luis A.; Tinnin, David S.; Muñoz, Carlos; de Mosca, Itza B.; Guerrero, Gladys; Kant, Rudick; Garcia, Arsenio; Caceres, Lorenzo; Gracia, Fernando G.; Quiroz, Evelia; de Castillo, Zoila; Armien, Blas; Libel, Marlo; Mills, James N.; Khan, Ali S.; Nichol, Stuart T.; Rollin, Pierre E.; Ksiazek, Thomas G.; Peters, Clarence J.

    2004-01-01

    An outbreak of hantavirus pulmonary syndrome occurred in the province of Los Santos, Panama, in late 1999 and early 2000. Eleven cases were identified; 9 were confirmed by serology. Three cases were fatal; however, no confirmed case-patient died. Case-neighborhood serologic surveys resulted in an overall hantavirus antibody prevalence of 13% among household and neighborhood members from the outbreak foci. Epidemiologic investigations did not suggest person-to-person transmission of hantavirus infection. By use of Sin Nombre virus antigen, hantavirus antibodies were detected in Oligoryzomys fulvescens and Zygodontomys brevicauda cherriei. This outbreak resulted in the first documented cases of human hantavirus infections in Central America. PMID:15498167

  5. A 4D-variational ocean data assimilation application for Santos Basin, Brazil

    NASA Astrophysics Data System (ADS)

    da Rocha Fragoso, Mauricio; de Carvalho, Gabriel Vieira; Soares, Felipe Lobo Mendes; Faller, Daiane Gracieli; de Freitas Assad, Luiz Paulo; Toste, Raquel; Sancho, Lívia Maria Barbosa; Passos, Elisa Nóbrega; Böck, Carina Stefoni; Reis, Bruna; Landau, Luiz; Arango, Hernan G.; Moore, Andrew M.

    2016-03-01

    Aiming to achieve systematic ocean forecasting for the southeastern Brazilian coast, an incremental 4D-Var data assimilation system is applied to a regional ocean model focused mainly in the Santos Basin region. This implementation is performed within the scope of The Santos Basin Ocean Observing System (or Project Azul), a pilot project designed to collect oceanographic data with enough frequency and spatial coverage so to improve regional forecasts through data assimilation. The ocean modeling and data assimilation system of Project Azul is performed with the Regional Ocean Modeling System (ROMS). The observations used in the assimilation cycles include the following: 1-day gridded, 0.1° resolution SST from POES AVHRR; 1-day gridded, 0.3° composite of the MDT SSH from AVISO; and surface and subsurface hydrographic measurements of temperature and salinity collected with gliders and ARGO floats from Project Azul and from UK Met-Office EN3 project dataset. The assimilative model results are compared to forward model results and independent observations, both from remote sensing and in situ sources. The results clearly show that 4D-Var data assimilation leads to an improvement in the skill of ocean hindcast in the studied region.

  6. A Comparative Analysis of Oral Health on the Santo Domingo Pueblo Reservation.

    PubMed

    Batliner, Terrence; Wilson, Anne; Davis, Elaine; Gallegos, Joaquin; Thomas, Jacob; Tiwari, Tamanna; Fehringer, Karen; Wilson, Katherine; Albino, Judith

    2016-06-01

    The study was done to compare oral health data from a tribe in a relatively accessible location between Santa Fe and Albuquerque, New Mexico to national American Indian data and broader US data sets. Participants (N = 399) were recruited via random sampling of housing units. Dental health measures included DMFT/dmft and dental sealants. Comparisons were made using data from large-scale oral health surveillance studies. There was no difference in oral health for 3-5 year olds compared to a recent study of AI/AN preschool children. Compared to the general US population, Santo Domingo Pueblo children and adults showed higher prevalence of untreated decay. Children ages 5-19 had higher rates of sealant retention on permanent teeth, and adults showed lower prevalence of complete tooth retention. The children ages 5-19 and 12-19 with at least one sealant have significantly lower DMFT and less untreated decay than those without sealants. However, the percentage of children with and without sealants who had untreated decay was still more than two times higher than the general US population. Oral health of American Indian children and adults in Santo Domingo Pueblo was worse compared to the general US population but similar to previous results reported for the same Indian Health Service Area even though their location is less isolated than many other tribes. PMID:26611694

  7. Metal concentration in muscle of two species of flatfish from Santos Bay, Southeastern Brazilian coast

    NASA Astrophysics Data System (ADS)

    Rocha, M. L. F.; Dias, J. F.; Boufleur, L. A.; Santos, C. E. I.; Dias, J. F.

    2014-01-01

    The aim of this study was to investigate metals in muscles of two species of flatfish, using Particle Induced X-ray Emission (PIXE). Specimens were caught monthly throughout the year 2005. Sampling was done at six points in the Santos Bay under different anthropogenic influences. Analysis of 56 samples of muscle showed detectable amounts of Al, As, Pb, Cu, Cr, Fe, Sr, Mn, Hg, Ni, Se and Zn. Except Cu, there were no correlations with the levels of sediment contamination by metals and those found in muscles. Moreover, there were significant differences between juveniles and adults concerning the concentration of Al, Mn and As. According to Brazilian Health Surveillance Agency, some elements detected in the samples of muscle were above of permitted by law for consumption: Hg and Pb (in samples from the internal area), As (from the west side) and Se and Cr (from east side of the bay).

  8. Shellfish from Todos os Santos Bay, Bahia, Brazil: treat or threat?

    PubMed

    de Souza, Manuel M; Windmöller, Cláudia C; Hatje, Vanessa

    2011-10-01

    This study determined the concentrations of major and trace elements in shellfish (oysters, clams and mussels) and conducted an assessment of the health risks due to the consumption of contaminated seafood. Samples were collected at 34 sites along Todos os Santos Bay, Brazil. The elements were determined by ICP OES and Hg by Direct Mercury Analysis. Relatively high concentrations of trace elements (As, Zn, Se and Cu) were found in seafood tissues. Potential daily intake of As, Co, Se, Zn and Cu associated to shellfish consumption suggested relevant non-carcinogenic risk for all studied locations. Copper was the element that posed the greatest non-carcinogenic risk, while Pb posed the highest carcinogenic risk. Health risks for humans were greatest from the consumption of mussels. Contaminated shellfish offer the greatest risk for children, subsistence fishers and subsistence shellfish consumers. PMID:21803378

  9. [Enteroparasitoses in the V Region, Chile. A study of rural school children from Santo Domingo, 1987].

    PubMed

    Neira, P; Muñoz, N; Carabelli, M; Subercaseaux, B; Tardío, M T; Villalón, L; Herrera, G

    1990-01-01

    The results of a new enteroparasitological survey carried out by the authors are analyzed with the aim of contributing to the knowledge of the situation of these infections in the V Region, Chile. In 1987 the children of five rural schools of Santo Domingo were studied by means of the modified Telemann method, the Ziehl-Neelsen stain and seried Graham test. The parasites more frequently found were: E. vermicularis (50.4%) and G. lamblia (10.8%). No E. histolytica was found in these children. The most frequent commensal was E. nana (21.9%). Cryptosporidium sp. presented a low frequency (0.9%), a figure that in these asymptomatic subjects is in accordance with that found in outpatients with chronic diarrhea in Valparaíso, V Region. PMID:1726862

  10. Paleomagnetism of the Todos Santos Formation in the Maya Block, Chiapas, Mexico: Preliminary Results

    NASA Astrophysics Data System (ADS)

    Godinez-Urban, A.; Molina-Garza, R. S.; Iriondo, A.; Geissman, J. W.

    2008-12-01

    Preliminary results of a paleomagnetic study on jurassic volcanic rocks (U-Pb 188.8 +/- 3.2Ma) locally interbedded with red beds assigned to the Todos Santos Formation, sampled in the Homoclinal Tectonic Province of the Neogene Fold Belt, Chiapas-Mexico, reveal multi component magnetizations acquired during pre- and post- folding of these rocks. The samples responded well to thermal demagnetization, but not so to AF demagnetization, suggesting that a high coercivity mineral phase like hematite is the main remanence carrier. The post-folding B-component direction of Dec=174.3 Inc=-30.6 (k=46; alpha95=13.6; N=4) represents a recent Tertiary? overprint; while the pre-folding C-component direction of Dec=329.9 Inc=7.8 (k=12.5; alpha95=16.3; N=8) is in agreement with a previously reported small data set for the Todos Santos Formation. When compared to the North American reference direction (Jurassic Kayenta Formation) the observed direction indicates a counterclockwise rotation of 35.9 +/- 16.6 degrees, and moderate north to south latitudinal displacement. If a reference pole from NE North America is used, the amount of counterclockwise rotation and latitudinal displacement are both slightly reduced. If the assumption that Jurassic strata in Chiapas reflect displacement of the Maya Block, then these data are consistent with reconstructions of the Maya Block in the Gulf of Mexico region. Other sites sampled in Jurassic strata suggest that in addition to the interpreted regional rotation, local (vertical-axis) rotations may have affected the region in more recent times.

  11. Diseases Neglected by the Media in Espírito Santo, Brazil in 2011–2012

    PubMed Central

    Vasconcellos-Silva, Paulo Roberto; Oliveira, Adauto Emmerich

    2016-01-01

    Background The aims of the present study were to identify and analyse the Diseases Neglected by the Media (DNMs) via a comparison between the most important health issues to the population of Espírito Santo, Brazil, from the epidemiological perspective (health value) and their effective coverage by the print media, and to analyse the DNMs considering the perspective of key journalists involved in the dissemination of health topics in the state media. Methodology Morbidity and mortality data were collected from official documents and from Health Information Systems. In parallel, the diseases reported in the two major newspapers of Espírito Santo in 2011–2012 were identified from 10,771 news articles. Concomitantly, eight interviews were conducted with reporters from the two newspapers to understand the journalists’ reasons for the coverage or neglect of certain health/disease topics. Principal Findings Quantitatively, the DNMs identified diseases associated with poverty, including tuberculosis, leprosy, schistosomiasis, leishmaniasis, and trachoma. Apart from these, diseases with outbreaks in the period evaluated, including whooping cough and meningitis, some cancers, respiratory diseases, ischaemic heart disease, and stroke, were also seldom addressed by the media. In contrast, dengue fever, acquired immune deficiency syndrome (AIDS), diabetes, breast cancer, prostate cancer, tracheal cancer, and bronchial and lung cancers were broadly covered in the period analysed, corroborating the tradition of media disclosure of these diseases. Qualitatively, the DNMs included rare diseases, such as amyotrophic lateral sclerosis (ALS), leishmaniasis, Down syndrome, and verminoses. The reasons for the neglect of these topics by the media included the political and economic interests of the newspapers, their editorial line, and the organizational routine of the newsrooms. Conclusions Media visibility acts as a strategy for legitimising priorities and contextualizing

  12. Gravity and Magnetotelluric Modeling of the Santo Domingo Basin, Northern New Mexico

    NASA Astrophysics Data System (ADS)

    Zamudio, K. D.; Keithline, N.; Blum, C.; Cunningham, E.; Fromont, A.; Jorgensen, M.; Lee, R.; McBride, K.; Saez Berrios, P.; Harper, C.; Pellerin, L.; McPhee, D.; Ferguson, J. F.

    2015-12-01

    The Santo Domingo Basin, one of a series of basins within the Rio Grande Rift, is located between Santa Fe and Albuquerque, NM, and has been the focus of research by the Summer of Geophysical Experience (SAGE) program since 2000. Gravity, magnetotelluric (MT), and seismic data have been collected throughout the region, although we are concentrating on gravity and MT data collected during SAGE 2014 and 2015. The study area is located in the center of the Santo Domingo basin, an extensional, Miocene age, rift basin, in an area that was minimally involved in the preceding local Laramide orogenic activity. Rift sediments (~3.5 km thick) are underlain by Eocene age sediments that were shed from adjacent uplifts. Up to 3 km of Mesozoic and Paleozoic sediments are preserved above the Precambrian basement. Geologic outcrop, borehole and seismic reflection data, and known density values were used in the construction of a ~100 km-long, generalized geologic cross section from which a gravity response was calculated. The modeled gravity response makes fairly definitive predictions about the geometry of the basin as well as the stratigraphy and faulting within and bounding the basin. MT data was collected at ten stations within the basin. The MT sounding curves exhibit one-dimensional behavior at short periods (<10 s), not surprisingly considering the relatively flat local structure in the area. Layered-earth MT models, without geologic constraints, show a conductive (<10 ohm-m) layer at ~1.5 km above a more resistive layer (>1000 ohm-m) at ~ 3.5-4 km. Conductivities of the major stratigraphic units have been determined from well logs and previous MT modeling. Forward and inverse MT models constrained by the gravity-modeled geologic cross section are used to develop a conductivity model consistent with the geology, and are a step towards a better unified treatment of MT, seismic and gravity data.

  13. Deep structure of the Santos Basin-São Paulo Plateau System, SE Brazil

    NASA Astrophysics Data System (ADS)

    Evain, M.; Afilhado, A.; Rigoti, C.; Loureiro, A.; Alves, D.; Klingelhoefer, F.; Schnurle, P.; Feld, A.; Fuck, R.; Soares, J.; Lima, M. Vinicius; Corela, C.; Matias, L.; Benabdellouahed, M.; Baltzer, A.; Rabineau, M.; Viana, A.; Moulin, M.; Aslanian, D.

    2015-08-01

    The structure and nature of the crust underlying the Santos Basin-São Paulo Plateau System (SSPS), in the SE Brazilian margin, are discussed based on five wide-angle seismic profiles acquired during the Santos Basin (SanBa) experiment in 2011. Velocity models allow us to precisely divide the SSPS in six domains from unthinned continental crust (Domain CC) to normal oceanic crust (Domain OC). A seventh domain (Domain D), a triangular shape region in the SE of the SSPS, is discussed by Klingelhoefer et al. (2014). Beneath the continental shelf, a ~100 km wide necking zone (Domain N) is imaged where the continental crust thins abruptly from ~40 km to less than 15 km. Toward the ocean, most of the SSPS (Domains A and C) shows velocity ranges, velocity gradients, and a Moho interface characteristic of the thinned continental crust. The central domain (Domain B) has, however, a very heterogeneous structure. While its southwestern part still exhibits extremely thinned (7 km) continental crust, its northeastern part depicts a 2-4 km thick upper layer (6.0-6.5 km/s) overlying an anomalous velocity layer (7.0-7.8 km/s) and no evidence of a Moho interface. This structure is interpreted as atypical oceanic crust, exhumed lower crust, or upper continental crust intruded by mafic material, overlying either altered mantle in the first two cases or intruded lower continental crust in the last case. The deep structure and v-shaped segmentation of the SSPS confirm that an initial episode of rifting occurred there obliquely to the general opening direction of the South Atlantic Central Segment.

  14. SANTOS - a two-dimensional finite element program for the quasistatic, large deformation, inelastic response of solids

    SciTech Connect

    Stone, C.M.

    1997-07-01

    SANTOS is a finite element program designed to compute the quasistatic, large deformation, inelastic response of two-dimensional planar or axisymmetric solids. The code is derived from the transient dynamic code PRONTO 2D. The solution strategy used to compute the equilibrium states is based on a self-adaptive dynamic relaxation solution scheme, which is based on explicit central difference pseudo-time integration and artificial mass proportional damping. The element used in SANTOS is a uniform strain 4-node quadrilateral element with an hourglass control scheme to control the spurious deformation modes. Finite strain constitutive models for many common engineering materials are included. A robust master-slave contact algorithm for modeling sliding contact is implemented. An interface for coupling to an external code is also provided. 43 refs., 22 figs.

  15. Phylogeography of rabies virus isolated from herbivores and bats in the Espírito Santo State, Brazil.

    PubMed

    Vieira, Luiz Fernando Pereira; Pereira, Sílvia Regina Ferreira Gonçalves; Carnieli, Pedro; Tavares, Luiz Carlos Barbosa; Kotait, Ivanete

    2013-04-01

    Rabies is enzootic in the State of Espírito Santo, Brazil. Every year, cattle and horses die from rabies that is transmitted by the vampire bat Desmodus rotundus. This paper describes the spread of the rabies virus by the continuous diffusion model using relaxed random walks with BEAST software. Forty-one (41) sequences of gene G from the rabies virus that was isolated from bats and domestic herbivores from several areas of the state between 2006 and 2010 were analyzed. The phylogenetic tree showed three main clusters as well as two sub-clusters under cluster 2. A spatial analysis showed that three strains of the rabies virus spread independently. In general, central Espírito Santo, which is mountainous, was the area where separation of the virus strains occurred. This physical barrier, however, was overcome at some point in time, as samples from different lineages were found in the same microarea. PMID:23264105

  16. Authigenic chlorite as a controlling factor in the exploration of Turonian/Coniacian turbidites in the Santos Basin

    SciTech Connect

    Porsche, E.; Lopes de Freitas, E.

    1996-08-01

    Upper Turonian/Coniacian and Campanian turbidites are major targets for petroleum exploration in the Santos Basin, southeastern Brazil. They occur between 140 and 1000 m of present water depth, are buried at about 4500 m, and reach thickness of up to 60 m. The main reservoir facies is composed of unstratified, fine to very fine grained, poorly sorted sandstones, which framework is compositionally immature, including a high proportion of feldspars and volcanic rock fragments. Early coating of grains by authigenic chlorite inhibited pressure solution and quartz cementation in the reservoir. This diagenetic characteristic allowed important preservation of primary porosity (>20%) in the reservoir; nevertheless its permeability never exceeds 30 ml. The study of sedimentary facies and related depositional processes has been conducted to predict the distribution of petroleum-bearing turbidites throughout the Santos Basin; this comprises a major challenge for the petroleum exploration in this important Brazilian exploration frontier.

  17. Integrated quality assessment of sediments from harbour areas in Santos-São Vicente Estuarine System, Southern Brazil

    NASA Astrophysics Data System (ADS)

    Buruaem, Lucas Moreira; de Castro, Ítalo Braga; Hortellani, Marcos Antonio; Taniguchi, Satie; Fillmann, Gilberto; Sasaki, Silvio Tarou; Varella Petti, Mônica Angélica; Sarkis, Jorge Eduardo de Souza; Bícego, Márcia Caruso; Maranho, Luciane Alves; Davanso, Marcela Bergo; Nonato, Edmundo Ferraz; Cesar, Augusto; Costa-Lotufo, Leticia Veras; Abessa, Denis Moledo de Souza

    2013-09-01

    Santos-São Vicente Estuarine System is a highly populated coastal zone in Brazil and where it is located the major port of Latin America. Historically, port activities, industrial and domestic effluents discharges have constituted the main sources of contaminants to estuarine system. This study aimed to assess the recent status of sediment quality from 5 zones of Port of Santos by applying a lines-of-evidence approach through integrating results of: (1) acute toxicity of whole sediment and chronic toxicity of liquid phases; (2) grain size, organic matter, organic carbon, nitrogen, phosphorus, trace metals, polycyclic aromatic hydrocarbons, linear alkylbenzenes and butyltins; (3) benthic community descriptors. Results revealed a gradient of increasing contamination for metals and organic compounds, alongside with their geochemical carriers. Sediment liquid phases were more toxic compared to whole sediment. Low number of species and individuals indicated the impoverishment of benthic community. The use of site-specific sediment quality guidelines was more appropriate to predict sediment toxicity. The integration of results through Sediment Quality Triad approach and principal component analysis allowed observing the effects of natural stressors and dredging on sediment quality and benthic distribution. Even with recent governmental efforts to control, pollution is still relevant in Port of Santos and a threat to local ecosystems.

  18. Human sporotrichosis beyond the epidemic front reveals classical transmission types in Espírito Santo, Brazil.

    PubMed

    de Araujo, Mariceli L; Rodrigues, Anderson M; Fernandes, Geisa F; de Camargo, Zoilo P; de Hoog, G Sybren

    2015-08-01

    Sporotrichosis has emerged as the main subcutaneous mycosis of humans and animals around the world. With particular differences in frequency, the major species includes Sporothrix brasiliensis, S. schenckii, S. globosa and S. luriei. In Brazil, the main aspect of this epidemic is based on the zoonotic transmission through the scratches and bites of diseased cats contaminated with S. brasiliensis. Areas free of feline sporotrichosis are poorly characterised in Brazil. We investigated by molecular tools the epidemiology of human sporotrichosis in the Espírito Santo (ES) state, an area adjacent to Rio de Janeiro where is the epicentre of the long-lasting outbreak of cat-transmitted sporotrichosis. The human cases in the ES state reveal the prevalence of classical transmission types where subjects are mainly infected by accidental traumatic inoculation during manipulation of contaminated plant material. In agreement with an environmental source, Sporothrix schenckii was the major aetiological agent in the classical transmission. Unlike Rio de Janeiro, this study shows that cat-transmitted epidemic in Espírito Santo is still scanty, although the geographic proximity and similar climatic features. Sporothrix brasiliensis was the agent in the feline-transmitted cases. Sporothrix globosa was isolated from a patient with fixed cutaneous lesions that did not report any contact with diseased animals. In conclusion, beyond the borders of Rio de Janeiro epidemic, agents of sporotrichosis in Espírito Santo show a scattered occurrence with high species diversity. PMID:26156862

  19. Characterization of Coffea arabica monofloral honey from Espírito Santo, Brazil.

    PubMed

    Kadri, Samir Moura; Zaluski, Rodrigo; Pereira Lima, Giuseppina Pace; Mazzafera, Paulo; de Oliveira Orsi, Ricardo

    2016-07-15

    In this study, samples of coffee honey produced in Espírito Santo State, Brazil, were characterized based on their melissopalynology, physicochemical and nutritional properties, and mineral and caffeine contents. The caffeine content in the nectar from coffee flowers was measured by high performance liquid chromatography (HPLC). Blends of honey were obtained from three Coffea arabica crops, each with 10 colonies of Africanized Apis mellifera. All honey samples contained monofloral (75-78%) pollen belonging to C. arabica. Physicochemical parameters (total acidity, pH, moisture, dry matter, ash, and qualitative hydroxymethylfurfural) were within the approved limits established by EU legislation. Coffee honey contains high levels of ascorbic acid (294.68 mg kg(-1)) and low amounts of total flavonoids (3.51 ± 0.18 mg QE kg(-1)). The most abundant minerals were potassium and calcium (962.59 ± 154.3 and 343.75 ± 25.56 mg kg(-1), respectively). The caffeine content in coffee nectar (1.64 mg kg(-1)) was approximately 8-fold lower than that in honey (12.02 ± 0.81 mg kg(-1)). PMID:26948612

  20. Determinants of health in seasonal migrants: coffee harvesters in Los Santos, Costa Rica.

    PubMed

    Loría Bolaños, Rocío; Partanen, Timo; Berrocal, Milena; Alvárez, Benjamín; Córdoba, Leonel

    2008-01-01

    In the agroexport zone of Los Santos Zone in Costa Rica, coffee is harvested by migrant labor. Most migrants are from Panama and Nicaragua. We describe migrants' housing- and service-related health determinants, with analyses of ethnicity, nationality and geography. We used interviews, observation-based assessments, and the Geographic Information System to assess a population of 8,783 seasonal migrants and 1,099 temporary dwellings at a total of 520 farms during 2004-2005. We identified determinants of poor health including widespread deficiencies in the quality of grower-provided dwellings, geographical isolation, crowding, lack of radio and television, and deficient toilets and cooking facilities. The indigenous and non-Costa Ricans shared the poorest conditions. Reluctance to use mainstream public health services was widespread, especially among foreign and indigenous migrants and the geographically isolated. Post-study, researchers organized workshops for audiences including workers, coffee producers, public officials and service providers. Topics have included migration, preventive health and hygiene, and child labor. This work was successful in convincing Costa Rican social security authorities to implement reforms that improve access to and quality of health care for the migrants. Special projects on ergonomics, psychosocial health hazards, and water quality, as well as a literacy program, are ongoing. PMID:18507290

  1. Survey for Tick-Borne Zoonoses in the State of Espirito Santo, Southeastern Brazil

    PubMed Central

    Spolidorio, Mariana G.; Labruna, Marcelo B.; Machado, Rosangela Z.; Moraes-Filho, Jonas; Zago, Augusto M.; Donatele, Dirlei M.; Pinheiro, Sônia R.; Silveira, Iara; Caliari, Késia M.; Yoshinari, Natalino H.

    2010-01-01

    Blood samples collected from 201 humans, 92 dogs, and 27 horses in the state of Espirito Santo, Brazil, were tested by polymerase chain reaction, indirect immunofluorescence assays, and indirect enzyme-linked immunosorbent assay for tick-borne diseases (rickettsiosis, ehrlichiosis, anaplasmosis, borreliosis, babesiosis). Our results indicated that the surveyed counties are endemic for spotted fever group rickettsiosis because sera from 70 (34.8%) humans, 7 (7.6%) dogs, and 7 (25.9%) horses were reactive to at least one of the six Rickettsia species tested. Although there was evidence of ehrlichiosis (Ehrlichia canis) and babesiosis (Babesia canis vogeli, Theileria equi) in domestic animals, no human was positive for babesiosis and only four individuals were serologically positive for E. canis. Borrelia burgdorferi-serologic reactive sera were rare among humans and horses, but encompassed 51% of the canine samples, suggesting that dogs and their ticks can be part of the epidemiological cycle of the causative agent of the Brazilian zoonosis, named Baggio-Yoshinari Syndrome. PMID:20595502

  2. Enrichment Factors (EF) on Superficial Sediments of Santos Estuarine System, Southeasthern Brazil

    NASA Astrophysics Data System (ADS)

    Kim, B. S. M.; Salaroli, A. B.; Mahiques, M.; Figueira, R.

    2014-12-01

    The Baixada Santista, located at southeastern Brazilian coast, hosts the largest harbor of South America, it is also the third most heavily populated region in São Paulo state. Many industries and domestic sewage have been contaminating the environment with heavy metals and arsenic since industrial revolution. This has been a major concern worldwide due to its toxicity and persistence. Levels of metals (Al, Cd, Cr, Cu, Ni, Pb, Zn, Fe, Mn, Sc, V) and As were determinated on 180 samples of surface sediments, by acid digestion and quantified by ICP-OES to assess enrichment factors in order to know the anthropogenic contribution of the investigated elements. The levels of metals indicated absence of contamination, but a higher enrichment of As (approximately 9.5) near Bertioga city. Ni and Cr had values between 0.5 - 2.0, Cu and Zn between 0.6 - 4.0 and 0.9 - 7.5 for Pb. Despite As, all others metals showed higher enrichment in Santos - São Vicente Estuary, especially near Cubatão. The analysis of the enrichment factor of As indicate that these values could be due to natural processes of weathering and sedimentation, meanwhile the other metals could be from antropogenic sources, since thoses values were found near industrial area.

  3. Mitochondrial DNA control region diversity in a population from Espirito Santo state, Brazil.

    PubMed

    Sanches, Naiara M; Paneto, Greiciane G; Figueiredo, Raquel F; de Mello, Aline O; Cicarelli, Regina M B

    2014-10-01

    Mitochondrial DNA (mtDNA) analysis has proved to be useful for forensic identification, especially in cases which nuclear DNA markers fail, as in degraded samples or in cases where the biological material has few traces or no nuclear DNA. Moreover, it can be applied in population genetics, inferring the origin of a population. In this work, the entire mtDNA control region of 97 individuals from the state of Espirito Santo, Brazil, was analyzed. We have found 94 different haplotypes yielding a high haplotype diversity of 0.9994 ± 0.0016. The probability of a random match calculated was 1.09. Haplogroup distribution analysis confirmed a highly admixed Latin American population: African lineages (43.3 %), European lineages (32.0 %), Native American lineages (23.7 %) and Asian lineages (1.0 %). We have concluded that this type of tool can be used both in forensic genetics to the study of different human populations, such as highly admixed populations, and in the study of migration's history and colonization of different states and countries of the world. PMID:24996288

  4. Geology and mineralogy of the Santo Nino Ag-Pb-Zn vein, Fresnillo District, Mexico

    SciTech Connect

    Gemmell, J.B.; Zantop, H.; Birnie, R.W.

    1985-01-01

    The Santo Nino Ag-Pb-Zn vein is the major producer of the Fresnillo District, located 750 km NW of Mexico City. It is over 2.4 km long, more than 480 m in vertical extent, more than 2.5 m wide overall, and has average grades of >600 gm/t Ag and <2% combined Pb and Zn. The vein is hosted by a tilted sequence of Cretaceous graywackes, shales and andesitic volcanics and extends upward into a Lower Tertiary conglomerate. Up to 5 separate opening events occurred along the vein, resulting in discontinuous stages of brecciation and crustiform banding. Ore mineral zonation is well developed both vertically and laterally and closely reflects metal and metal ratio distributions. Ore minerals are sphalerite, galena, pyrite, chalcopyrite, arsenopyrite, marcasite, pyrrhotite, acanthite, native silver, and three coexisting solid solution series, pyrargyrite-proustite, polybasite-arsenopolybasite, and tetrahedrite-tennantite in a gangue of quartz, calcite, clay, sericite,and chlorite. A 5-stage paragenetic sequence can be established: 1) pyrite, arsenopyrite, quartz, 2) sphalerite, galena, chalcopyrite, quartz, 3) tetrahedrite, pyrargyrite, polybasite, quartz, 4) acanthite, native silver, calcite, quartz, and 5) calcite. Preliminary microprobe analyses indicate that the Ag-rich solid solution series are Sb-rich in the central and upper portions of the vein and As-rich at deeper levels.

  5. Dynamic changes in microbiota and mycobiota during spontaneous 'Vino Santo Trentino' fermentation.

    PubMed

    Stefanini, Irene; Albanese, Davide; Cavazza, Agostino; Franciosi, Elena; De Filippo, Carlotta; Donati, Claudio; Cavalieri, Duccio

    2016-03-01

    Vino Santo is a sweet wine produced from late harvesting and pressing of Nosiola grapes in a small, well-defined geographical area in the Italian Alps. We used metagenomics to characterize the dynamics of microbial communities in the products of three wineries, resulting from spontaneous fermentation with almost the same timing and procedure. Comparing fermentation dynamics and grape microbial composition, we show a rapid increase in a small number of wine yeast species, with a parallel decrease in complexity. Despite the application of similar protocols, slight changes in the procedures led to significant differences in the microbiota in the three cases of fermentation: (i) fungal content of the must varied significantly in the different wineries, (ii) Pichia membranifaciens persisted in only one of the wineries, (iii) one fermentation was characterized by the balanced presence of Saccharomyces cerevisiae and Hanseniaspora osmophila during the later phases. We suggest the existence of a highly winery-specific 'microbial-terroir' contributing significantly to the final product rather than a regional 'terroir'. Analysis of changes in abundance during fermentation showed evident correlations between different species, suggesting that fermentation is the result of a continuum of interaction between different species and physical-chemical parameters. PMID:26780037

  6. Air pollution and its impacts on health in Vitoria, Espirito Santo, Brazil

    PubMed Central

    de Freitas, Clarice Umbelino; de Leon, Antonio Ponce; Juger, Washington; Gouveia, Nelson

    2016-01-01

    ABSTRACT OBJECTIVE To analyze the impact of air pollution on respiratory and cardiovascular morbidity of children and adults in the city of Vitoria, state of Espirito Santo. METHODS A study was carried out using time-series models via Poisson regression from hospitalization and pollutant data in Vitoria, ES, Southeastern Brazil, from 2001 to 2006. Fine particulate matter (PM10), sulfur dioxide (SO2), and ozone (O3) were tested as independent variables in simple and cumulative lags of up to five days. Temperature, humidity and variables indicating weekdays and city holidays were added as control variables in the models. RESULTS For each increment of 10 µg/m3 of the pollutants PM10, SO2, and O3, the percentage of relative risk (%RR) for hospitalizations due to total respiratory diseases increased 9.67 (95%CI 11.84-7.54), 6.98 (95%CI 9.98-4.17) and 1.93 (95%CI 2.95-0.93), respectively. We found %RR = 6.60 (95%CI 9.53-3.75), %RR = 5.19 (95%CI 9.01-1.5), and %RR = 3.68 (95%CI 5.07-2.31) for respiratory diseases in children under the age of five years for PM10, SO2, and O3, respectively. Cardiovascular diseases showed a significant relationship with O3, with %RR = 2.11 (95%CI 3.18-1.06). CONCLUSIONS Respiratory diseases presented a stronger and more consistent relationship with the pollutants researched in Vitoria. A better dose-response relationship was observed when using cumulative lags in polynomial distributed lag models. PMID:26982960

  7. Occurrence and significance of microbialites in the uplifted Tasmaloum reef (SW Espiritu Santo, SW Pacific)

    NASA Astrophysics Data System (ADS)

    Cabioch, Guy; Taylor, Frederick W.; Corrège, Thierry; Récy, Jacques; Edwards, Lawrence R.; Burr, George S.; Le Cornec, Florence; Banks, Kirsten A.

    1999-07-01

    In the SW Pacific Ocean, subduction of the d'Entrecasteaux ridge system has caused rapid uplift of the central New Hebrides Island Arc. The maximum uplift rate of 6 mm yr -1 occurs along the southwest coast of Espiritu Santo Island, near Tasmaloum. The Tasmaloum uplifted reef sequence internal structure, which is strongly linked to its tectonic context, was investigated through a series of drill-holes to depths up to 42 m. Although a stable tropical coast would undergo approximately 120 m of post-glacial sea-level rise, the net relative sea-level rise on such a rapidly uplifting coast is only about 20 m. Colonization of the Tasmaloum fringing reef occurred by 24 ka, upon a pre-reef substrate composed of a thick bioclastic sand formation accumulated during the last glacial period. During the post-glacial sea-level rise, the vertical succession of reef assemblages reflects environmental and bathymetric variations controlled by the interplay of rapid, but variable rates of sea-level rise and more or less constant uplift of 5-6 mm yr -1. Microbialite crusts, composed of high-magnesian calcite laminae, occur in the Tasmaloum reef from 20 to 6 ka and are particularly abundant from 16 to 10 ka. The development of microbialite crusts is related to nutrient enrichment of interstitial waters through mixing with meteoric groundwater. After 6 ka, when sea level ceased rising in the region and continuing uplift caused rapid emergence of the reef, microbialites disappear within the subtidal assemblages. Several explanations can be put forward for their disappearance. In particular, nutrient input changes are a likely cause. A new hydrologic and oceanographic regime was established when sea level ceased rising. This change was accompanied by warming of tropical waters.

  8. Family meals and eating practices among mothers in Santos, Brazil: A population-based study.

    PubMed

    Sato, Priscila de Morais; Lourenço, Bárbara Hatzlhoffer; Trude, Angela Cristina Bizzotto; Unsain, Ramiro Fernandez; Pereira, Patrícia Rocha; Martins, Paula Andrea; Scagliusi, Fernanda Baeza

    2016-08-01

    This study investigates family meals among mothers and explores associations between eating with family and sociodemographic characteristics, body mass index, and eating practices. A population-based cross-sectional study, using complex cluster-sampling, was conducted in the city of Santos, Brazil with 439 mothers. Frequency of family meals was assessed by asking if mothers did or did not usually have a) breakfast, b) lunch, and c) dinner with family. Linear regression analyses were conducted for the number of meals eaten with family per day and each of the potential explanatory variables, adjusting for the mother's age. Poisson regression with robust variance was used to analyze each factor associated with eating with family as classified categorically: a) sharing meals with family, b) not eating any meals with family. Only 16.4% (n = 72) of participants did not eat any meals with family. From the 83.6% (n = 367) of mothers that had at least one family meal per day, 69.70% (n = 306) ate dinner with their families. Mothers aged ≥40 years reported significantly fewer meals eaten with family compared to mothers aged 30-39 years (β: -0.26, p = 0.04). Having family meals was 54% more prevalent among mothers with ≥12 years of education (PR for no meals eaten with family: 0.54, 95% CI: 0.30; 0.96, p = 0.03), when compared to mothers with less than nine years of education. Eating no meals with family was 85% more prevalent among mothers who reported that eating was one of the biggest pleasures in their lives (PR: 1.85, 95% CI: 1.21; 2.82, p = 0.004). We suggest the need for further research investigating the effects of family meals on mothers' health through nutritional and phenomenological approaches. PMID:26994738

  9. Bioclimatic distribution and prevalence maps for Fasciola hepatica in Espírito Santo State, Brazil

    PubMed Central

    2014-01-01

    Background Fasciolosis affects different ruminant species and leads to great economic losses for cattle farmers worldwide. Thus, the current study aimed to evaluate bovine fasciolosis prevalence in the state of Espírito Santo, Brazil, using slaughter maps provided by slaughterhouses and verifying the origin of cattle. Methods A map was created based on analysis of epidemiological data. The ArcGIS/ArcINFO 10.1 software was employed in order to elaborate updated bioclimatic maps that displayed the fasciolosis prevalence within the state – per city– between 2009 and 2011. Results According to the bioclimatic map it was clear that 52.24% of the state’s total area comprise regions considered favorable for the development and survival of Fasciola hepatica. According to the data provided by slaughterhouses, the parasite was more frequent in the cities of Atílio Vivácqua, Itapemirim and Anchieta with respective prevalence of 28.41, 25.50 and 24.95%. Although the northern portion of the state is also favorable for the disease maintenance (reaching rates above 90%), several cities presented prevalence of only 0.99 and 1.94% respectively. These findings indicate that climatic and environmental factors only cannot be considered preponderant to fasciolosis occurrence. Regarding the slaughterhouse located in Anchieta city, the higher prevalence was registered in the cities of Jerônimo Monteiro, Alegre and Cachoeiro de Itapemirim, with mean prevalence of 1.21, 1.07 and 2.09% respectively. Conclusion Although the present findings suggest a pattern for the prevalence of fasciolosis, records of the cities for the occurrence of the disease usually do not reflect the true origin of animals. PMID:25101121

  10. Deep structure of the Santos Basin-São Paulo Plateau System, SE Brazil

    NASA Astrophysics Data System (ADS)

    Evain, Mikael; Afilhado, Alexandra; Rigoti, Caesar; Loureiro, Afonso; Alves, Daniela; Klingelhoefer, Frauke; Schnurle, Philippe; Feld, Aurelie; Fuck, Reinhardt; Soares, Jose; Vinicius de Lima, Marcus; Corela, Carlos; Matias, Luis; Benabdellouahed, Massinissa; Baltzer, Agnes; Rabineau, Marina; Viana, Adriano; Moulin, Maryline; Aslanian, Daniel

    2015-04-01

    The structure and nature of the crust underlying the Santos Basin-São Paulo Plateau System (SSPS), in the SE Brazilian margin, is discussed based on five wide-angle seismic profiles acquired during the SanBa experiment in 2011. Velocity models allow us to precisely divide the SSPS in six domains from unthinned continental crust (Domain CC) to normal oceanic crust (Domain OC). A seventh domain (Domain D), a triangular shape region in the SE of the SSPS, is discussed by [Klingelhoefer et al., GJI, 2014]⁠. Beneath the continental shelf, a ~100 km wide necking zone (Domain N) is imaged where continental crust thins abruptly from ~40 km to less than 15 km. Toward the ocean, most of the SSPS (Domain A and C) shows velocity ranges, velocity gradients and a Moho interface characteristic of thinned continental crust. The central domain (Domain B) has, however, a very heterogeneous structure. While its southwestern part still exhibits extremely thinned (7 km) continental crust, its northeastern part depicts a 2-4 km thick upper layer (6.0-6.5 km/s) overlying an anomalous velocity layer (7.0-7.8 km/s) and no evidence of a Moho interface. This structure is interpreted as atypical oceanic crust, exhumed lower crust or upper continental crust intruded by mafic material, overlying either altered mantle in the first two cases or intruded lower continental crust in the last case. The v-shaped structuration in this central domain confirms an initial episode of rifting within the SSPS oblique to the general opening direction of the South Atlantic central segment.

  11. Prevalence and severity of wheezing in the first year of life in the city of Santo André, Brazil☆

    PubMed Central

    Ferreira, Isabel Cristina C.; Wandalsen, Neusa Falbo

    2014-01-01

    Objective: To determine the prevalence and the severity of wheezing in the first year of life for infants who live in Santo André, São Paulo, Brazil. Methods: Cross sectional study with the administration of the Estudio Internacional de Sibilancias en Lactantes (EISL), which is a standardized and validated written questionnaire applied to parents and/or guardians of infants aged 12-24 months treated at primary health units, vaccination centers, day care centers, or kindergartens. The questionnaire consisted of questions regarding demographic characteristics, presence of wheezing, respiratory infections, and risk factors. Results were analyzed using the SPSS for Windows, 20.0 (SPSS Inc. - Chicago, Il, United States). Logistic regression was applied to verify variables associated to recurrent wheezing. Results: Among the 1,028 infants studied, 48.5% had one or more episodes of wheezing during the first 12 months of life (wheezing once), and 23.9% had three or more episodes (recurrent wheezing). Nocturnal symptoms, severe breathing difficulty, and visits to the emergency room were observed in 67.3%, 42.4%, and 60.7% of infants, respectively. Among the studied infants, 19.4% were hospitalized, and 11.0% had a medical diagnosis of asthma in the first year of life. Use of β2-agonists, inhaled corticosteroids, oral corticosteroids, and leukotriene receptor antagonists were observed in 88.8%, 21.0%, 54.9%, and 3.2% of children with wheezing, respectively. Use of oral corticosteroids, perception of breathlessness by parents, diagnosis of asthma, pneumonia, and hospitalization for pneumonia were more frequent among infants with recurrent wheezing (p<0.001). Conclusions: In the city of Santo André, approximately half of infants had at least one episode of wheezing in the first year of life, and almost 25% had recurrent wheezing. Wheezing disorders in Santo André have early onset and high morbidity. PMID:25479844

  12. Cryptotermes (Isoptera, Kalotermitidae) on Espiritu Santo, Vanuatu: Redescription of Cryptotermes albipes (Holmgren & Holmgren) and description of Cryptotermes penaoru sp. n.

    PubMed Central

    Roisin, Yves

    2011-01-01

    Abstract Complete series of two species of the phragmotic drywood termite genus, Cryptotermes (Isoptera: Kalotermitidae), were found on Espiritu Santo, Vanuatu. Here, I describe for the first time the soldier of Cryptotermes albipes (Holmgren & Holmgren), which resembles Cryptotermes domesticus but presents deep depressions on the head sides and vertex. The other species, here described as Cryptotermes penaoru sp. n., comes close to Cryptotermes tropicalis, a species known from the tropical rainforest of northern Queensland, from which its soldier is distinguished by its more elongated head capsule. PMID:22287889

  13. Cryptotermes (Isoptera, Kalotermitidae) on Espiritu Santo, Vanuatu: Redescription of Cryptotermes albipes (Holmgren & Holmgren) and description of Cryptotermes penaoru sp. n.

    PubMed

    Roisin, Yves

    2011-01-01

    Complete series of two species of the phragmotic drywood termite genus, Cryptotermes (Isoptera: Kalotermitidae), were found on Espiritu Santo, Vanuatu. Here, I describe for the first time the soldier of Cryptotermes albipes (Holmgren & Holmgren), which resembles Cryptotermes domesticus but presents deep depressions on the head sides and vertex. The other species, here described as Cryptotermes penaorusp. n., comes close to Cryptotermes tropicalis, a species known from the tropical rainforest of northern Queensland, from which its soldier is distinguished by its more elongated head capsule. PMID:22287889

  14. Feasibility Study of Economics and Performance of Solar Photovoltaics at the Santo Domingo Pueblo in Sandoval County, New Mexico

    SciTech Connect

    Geiger, J.; Lisell, L.; Mosey, G.

    2013-07-01

    The U.S. Environmental Protection Agency (EPA), in accordance with the RE-Powering America's Land initiative, selected the Pueblo of Santo Domingo in Sandoval County, New Mexico, for a renewable energy production feasibility study. The National Renewable Energy Laboratory (NREL) provided technical assistance for this project. The purpose of this report is to assess specific areas on the Pueblo for potential installation of photovoltaic (PV) systems and to estimate the cost, performance, and site impacts of different PV options. The report also recommends financing options that could assist in the implementation of these PV systems.

  15. Effects of ayahuasca on psychometric measures of anxiety, panic-like and hopelessness in Santo Daime members.

    PubMed

    Santos, R G; Landeira-Fernandez, J; Strassman, R J; Motta, V; Cruz, A P M

    2007-07-25

    The use of the hallucinogenic brew ayahuasca, obtained from infusing the shredded stalk of the malpighiaceous plant Banisteriopsis caapi with the leaves of other plants such as Psychotria viridis, is growing in urban centers of Europe, South and North America in the last several decades. Despite this diffusion, little is known about its effects on emotional states. The present study investigated the effects of ayahuasca on psychometric measures of anxiety, panic-like and hopelessness in members of the Santo Daime, an ayahuasca-using religion. Standard questionnaires were used to evaluate state-anxiety (STAI-state), trait-anxiety (STAI-trait), panic-like (ASI-R) and hopelessness (BHS) in participants that ingested ayahuasca for at least 10 consecutive years. The study was done in the Santo Daime church, where the questionnaires were administered 1h after the ingestion of the brew, in a double-blind, placebo-controlled procedure. While under the acute effects of ayahuasca, participants scored lower on the scales for panic and hopelessness related states. Ayahuasca ingestion did not modify state- or trait-anxiety. The results are discussed in terms of the possible use of ayahuasca in alleviating signs of hopelessness and panic-like related symptoms. PMID:17532158

  16. Dead infants, cruel mothers, and heroic popes: the visual rhetoric of foundling care at the hospital of Santo Spirito, Rome.

    PubMed

    Presciutti, Diana Bullen

    2011-01-01

    The fresco cycle painted at the behest of Pope Sixtus IV in the late 1470s in the main ward of the hospital of Santo Spirito in rome comprises an extended pictorial biography of Sixtus, prefaced by scenes representing the legendary foundation of the hospital by his predecessor Innocent III. The legend, which tells how Innocent established Santo Spirito as a foundling hospital in response to the discovery of victims of infanticide in the Tiber River, positions the pope as the savior of the city's unwanted children. This article elucidates how the construction and renovation of the hospital is presented in the cycle as a generative product of papal will, with the care of foundlings situated as an integral part of the image of the pope as both Father of the Church and restorer of past glory to the city of Rome. While the frescoes engage with both widespread conventions for representing infanticide and commonplace notions of the social value of caring for abandoned children, I demonstrate that the ideologically potent visual rhetoric of foundling care was also flexible, and could be adapted to meet the specific needs of a particular institutional and patronal context. PMID:22165440

  17. O Universo das Sociedades Numa Perspectiva Relativa: Exercícios de Etnoastronomia

    NASA Astrophysics Data System (ADS)

    Fares, Érika Akel; Pessoa Martins, Karla; Maciel Araujo, Lidiane; Sauma Filho, Michel

    2004-12-01

    This work developed at the Planetarium of Pará employing workshops designed for visitor schools (public, private, specials groups), promotes the popularization of the Ethnoastronomy with the aim of diffusing values based on a cultural diversity tolerance and the need of harmonic interplay between people and environment. Using stories and debates, the relationship between humans and the Cosmos is traced from antiquity until today, with the help of slides as a didactic resource. Thus, many conceptions about the origin of the Universe are shown; as well as some constellations created along the History, ending with the present scientific manner of know and explain the World. We then demonstrate the links between space, time and culture with the Cosmos vision. The need to think the World within a plural context to create respect for other different persons is emphasized. Este trabalho, desenvolvido no Planetário do Pará através de oficinas com escolas visitantes (públicas, privadas e grupos especiais), promove a popularização da Etnoastronomia, com o objetivo de difundir valores pautados na tolerância à diversidade cultural e na necessidade da convivência harmônica entre o ser humano e o meio ambiente. Através da contação de histórias e promoção de debate, é tratada a relação dos humanos com o céu desde a antiguidade até os dias atuais, tendo como apoio didático o uso de slides. Assim, são mostradas diversas concepções de origem do Universo; algumas constelações criadas no decorrer da História; e a atual forma científica de conhecer e explicar o mundo. Demonstrando-se, então, a interligação entre espaço, tempo e cultura com a visão do Cosmo. Enfatiza-se a necessidade de se pensar o mundo numa perspectiva relativa ou plural, de forma a propiciar o respeito ao ser diferente.

  18. Owned dog demography in Todos Santos Cuchumatán, Guatemala.

    PubMed

    Pulczer, Andrew S; Jones-Bitton, Andria; Waltner-Toews, David; Dewey, Cate E

    2013-02-01

    A thorough knowledge of the dog demography should be an integral element in the planning, implementation and evaluation of dog population control measures. In May 2008, a door-to-door household census of human and owned canine populations was conducted in 12 contiguous neighbourhoods in the town of Todos Santos Cuchumatán, Guatemala. During the census, household and footpath data were recorded using a handheld Global Positioning System (GPS), and used to create digital census route maps, and perimeter and area estimates of the study region. Approximately 99% of all households (472/476) participated in the census, representing 2461 people in the overall estimated study region area of 80 hectares (ha). A total of 382 dogs were owned by 50.8% (240/472) of households, yielding means of 0.8 (382/472) owned dogs per household and 1.6 (382/240) dogs per dog-owning household. Of the total 382 owned dogs, 88.2% (337/382) were aged three months or older; of these, 68.5% (231/337) were reported as not normally being confined on the household property during the average day, and 9.7% (24/247) of the males and none of the females (0/81) were reported to be neutered. Of the households that owned female dogs, 89.7% (52/58) and 91.4% (53/58) reported that they would have females surgically or non-surgically neutered, respectively, if these services were available. Of the households that owned male dogs, 90.3% (176/195) and 92.3% (180/195) reported that they would have males surgically or non-surgically neutered, respectively, if these services were available. Approximately 72% (238/330) of owned dogs were vaccinated for rabies, and 80% (187/238) of these were males. The owned dog male:female ratio was 2.6:1 (275/107), the owned dog:human ratio was 1:6.4 (382/2461), and the absolute density was 478 (382/0.80) owned dogs/km(2). This knowledge of the owned dog demography was generated using simple means and has been of direct use in support of, and as baseline data for the planning

  19. Apatite Fission-Track Analysis of the Middle Jurassic Todos Santos Formation from Chiapas, Mexico.

    NASA Astrophysics Data System (ADS)

    Abdullin, Fanis; Solé, Jesús; Shchepetilnikova, Valentina; Solari, Luigi; Ortega-Obregón, Carlos

    2014-05-01

    The Sierra de Chiapas (SCH), located in the south of Mexico, is a complex geological province that can be divided on four different lithological or tectonic areas: (1) the Chiapas Massif Complex (CMC); (2) the Central Depression; (3) the Strike-slip Fault Province, and (4) the Chiapas Fold-and-thrust Belt. The CMC mostly consists of Permian granitoids and meta-granitoids, and represents the basement of the SCH. During the Jurassic period red beds and salt were deposited on this territory, related to the main pulse of rifting and opening of the Gulf of Mexico. Most of the Cretaceous stratigraphy contains limestones and dolomites deposited on a marine platform setting during the postrift stage of the Gulf of Mexico rift. During the Cenozoic Era took place the major clastic sedimentation along the SCH. According the published low-temperature geochronology data (Witt et al., 2012), SCH has three main phases of thermo-tectonic history: (1) slow exhumation between 35 and 25 Ma, that affected mainly the basement (CMC) and is probably related to the migration of the Chortís block; (2) fast exhumation during the Middle-Late Miocene caused by strike-slip deformation that affects almost all Chiapas territory; (3) period of rapid cooling from 6 to 5 Ma, that affects the Chiapas Fold-and-thrust Belt, coincident with the landward migration of the Caribbean-North America plate boundaries. The two last events were the most significant on the formation of the present-day topography of the SCH. However, the stratigraphy of the SCH shows traces of the existence of earlier tectonic events. This study presents preliminary results of apatite fission-track (AFT) dating of sandstones from the Todos Santos Formation (Middle Jurassic). The analyses are performed with in situ uranium determination using LA-ICP-MS (e.g., Hasebe et al., 2004). The AFT data indicate that this Formation has suffered high-grade diagenesis (probably over 150 ºC) and the obtained cooling ages, about 70-60 Ma

  20. Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil

    PubMed Central

    Vicentini, Fernando; Denadai, Wilson; Gomes, Yohanna Mayelle; Rose, Tatiana L.; Ferreira, Mônica S. R.; Le Moullac-Vaidye, Beatrice; Le Pendu, Jacques; Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira; Spano, Liliana Cruz

    2013-01-01

    Noroviruses (NoV) are the main etiological agents of gastroenteritis outbreaks worldwide and susceptibility to NoV infection has been related to the histo-blood group antigen (HBGA). This study aimed to determine the prevalence of NoV strains and to evaluate the HBGA phenotype and genotype of children from semi-isolated Quilombola communities, descendents of black slaves in Brazil. A total of 397 children up to eleven years old, with and without diarrhea, from Quilombola Communities in the Espirito Santo State, Brazil, were investigated for the presence of NoV from August 2007 to September 2009. Feces were collected from all the children, and blood from the NoV positive children. NoV was screened by reverse transcription-PCR with primers for the RNA-dependent RNA polymerase region; genogroup was determined by PCR with primers for the C and D regions and genotyped by sequencing. HBGA phenotype was performed by gel-spinning and FUT2 and FUT3 were analyzed by PCR or sequencing analysis. NoV were detected in 9.2% (12/131) of diarrheic and 1.5% (4/266) of non-diarrheic children (p<0.05, Fisher’s exact test). GI and GII genogroups were present in 12.5% and 87.5% of the samples, respectively. The following genotypes were characterized: GII.4 (25%), GII.12 (25%), GII.6 (12.5%) and GI.1 (6.3%), GI.3 (12.5%) and GI.4 (6.3%). Children infected with NoV showed the A (n = 6), O (n = 6), and B (n = 2) HBGA phenotypes, and 13 of them were classified as secretors (Se) and one as a non secretor (se). Mutations of Se40, 171,216,357,428,739,960 were found for the FUT2 gene and mutations of Le59, 202, 314 for the FUT3 gene. The only se child was infected by NoV GI, whereas the Se children were indiscriminately infected by GI or GII. This study showed rates of NoV infection in symptomatic and asymptomatic Quilombola children consistent with other studies. However, children under 12 months were seven times more affected than those between 1 and 5 years old. GII.12 was as

  1. A Future for the Excluded. Job Creation and Income Generation by the Poor: Clodomir Santos de Morais and the Organization Workshop.

    ERIC Educational Resources Information Center

    Carmen, Raff, Ed.; Sobrado, Miguel, Ed.

    This book, translated from Spanish, contains 20 chapters by various authors examining and expanding on the work of Clodomir Santos de Morais in educating and empowering the poor, mostly in Latin America, for entrepreneurship. One of the chapters is by de Morais. The following are included, organized in four sections: "Those Who Don't Eat and Those…

  2. SPCZ- and ENSO-related Salinity Variations Recorded in the Skeletal Geochemistry of a Porites Coral from Espiritu Santo, Vanuatu

    NASA Astrophysics Data System (ADS)

    Kilbourne, K. H.; Quinn, T. M.; Taylor, F. W.; Delcroix, T.; Gouriou, Y.

    2003-12-01

    Variations in sea surface temperature (SST) and sea surface salinity (SSS) are important components of climate variability in the tropical ocean-atmosphere system. Instrumental SST time series are much more readily available than are instrumental SSS time series, which are exceedingly rare. SSS variations are strongly linked to seawater δ 18O variations in the tropics, thus coral-based reconstructions of seawater δ 18O offer an opportunity to reconstruct the history of SSS variations in the tropical oceans. Seawater δ 18O is obtained by combining coral skeletal δ 18O, which varies in response to changes in seawater δ 18O and SST, with coral skeletal Sr/Ca, which varies in response to SST changes. This method has great potential for reconstructing past salinity variations. We explore this potential using a monthly-resolved, 65-year record of skeletal δ 18O and Sr/Ca variations in a Porites coral from Espiritu Santo, Vanuatu (16.0° S, 166.7° E). Santo is well positioned for such a study because ENSO-related climate variability strongly influences local salinity through changes in the position of the center of South Pacific Convergence Zone (SPCZ) convection/precipitation and associated salinity front movements. Moreover, a 24-year record of SSS variations is available from this region, which permits a ground-truth assessment of the robustness of the coral-based seawater δ 18O-SSS reconstruction. Investigation into different methods of combining coral δ 18O and Sr/Ca to reconstruct SSS reveals that the coral δ 18O anomaly time series provides the best fit to the SSS time series. A post-1976/1977 freshening trend in the δ 18O anomaly time series, which has been documented in other proxy time series, increases our confidence that this time series accurately reflects changes in the hydrologic balance in the western tropical Pacific. The Vanuatu coral δ 18O anomaly time series also correlates strongly with the Southern Oscillation Index, the Niño 3.4 SST

  3. Shelf-edge sedimentary systems off Rio de Janeiro State, northern Santos basin-Brazil

    NASA Astrophysics Data System (ADS)

    Maia, R. M. C.; Dos Reis, A. T.; Gorini, C.; Silva, C. G.; Rabineau, M.; Granjeon, D.

    2012-04-01

    elements provide a hint at a prevailing subsidence regime and effective sediment supply into the basin that clearly contrast with the conveyed idea of a sediment-starved and tectonic stable shelf. They naturally raise questions about the nature and origin of sediment supply, since no significant point siliciclastic fluvial source flows directly into the shelf. Stemming from that, we are forced to speculate about: (A) the role of neotectonic movements involving the Serra do Mar coastal mountain ranges to potentially source clastic influx into the basin during the Quaternary, or about the real importance of secondary drainage basins debouching today; and (B) the mechanical nature of a supposed subsidence during the Pliocene and the Quaternary time span (overloading ? sediment compaction ? thermal cooling ?). The interpretation of industrial seismic lines can provide the answers of many of these questions. The next step of this work is to make a stratigraphy model of the sedimentary systems of Santos basin to understand how the ancient creation of accommodation space can influence the recent sedimentary architecture and how is the change in sedimentary influx and the sedimentary records of different orders of cyclicity.

  4. Hydrography of Bahia Todos Santos, Baja California: Results of more than twenty five years of investigations.

    NASA Astrophysics Data System (ADS)

    Bustos-Serrano, H.; Canino-Herrera, S. R.; Morales-Chavez, R.; Martinez-Garcia, G. M.

    2007-05-01

    The first study of Bahia Todos Santos (BTS) was reported by Walton in 1955. We conducted oceanographic studies in BTS since 1979. The BTS has a connection with a coastal lagoon named Estero de Punta Banda (EPB), two islands at the western portion and the Port of Ensenada. The general hydrographic characteristics are: In winter the water became homogeneous, less saline (<33.4) and with low stability. During spring and summer a hydrographic structure of three layers is noticed. The deepest layer is composed of the southern California water mass with a relatively high salinity (>33.6) low temperature (13°C), low oxygen (~3mL L-1) and rich in nutrient concentration. The isothermal top layer has relatively high temperature (>17°C) and oxygen concentration (>6mL L-1). The intermediate transition layer (seasonal thermocline) has minimum salinity, maximum oxygen and high stability. During fall there is distribution of heat from the surface layer to the entire water column. The thermic waves propagate with decrease amplitude in ~3 months, from surface to bottom water. The California Current flow generally southward off the western United States and northern Mexico and is one of the major coastal upwelling of the word oceans. The upwelling events in BTS appear regularly at the SW portion and were typically characterized by an increase in pCO2, decrease of O2, increase of nutrients and a lower temperature. Upwelling activity increases surface nutrient availability causing rise in the primary productivity and hence increased zooplankton biomass. The annual upwelling event which had a maximum strength on May, the seasonal warming and cooling, and the water advection were the dominant modifying processes for the variability of seawater characteristics. Higher salinities are located close to the coastline and lower off the bay. The levels of oxygen, alkalinity, pH and chlorophyll indicate that the maximum concentration of phytoplankton is located at the center of the bay. The

  5. New records of rhodolith-forming species (Corallinales, Rhodophyta) from deep water in Espírito Santo State, Brazil

    NASA Astrophysics Data System (ADS)

    Henriques, Maria Carolina; Villas-Boas, Alexandre; Rodriguez, Rafael Riosmena; Figueiredo, Marcia A. O.

    2012-06-01

    Little is known about the diversity of non-geniculate coralline red algae (Rhodophyta, Corallinophycidae) from deep waters in Brazil. Most surveys undertaken in this country have been carried out in shallow waters. In 1994, however, the REVIZEE program surveyed the sustainable living resources potential of the Brazilian exclusive economic zone to depths of 500 m. In the present study, the rhodolith-forming coralline algae from the continental shelf of Espírito Santo State were identified. Samples were taken from 54 to 60 m depth by dredging during ship cruises in 1997. Three rhodolith-forming species were found: Spongites yendoi (Foslie) Chamberlain , Lithothamnion muelleri Lenormand ex Rosanoff and Lithothamnion glaciale Kjellman. These records extend the distribution ranges of these species into Brazilian waters and extend the depth distribution of non-geniculate coralline red algae into Brazilian water to 58 m.

  6. Tuberculosis and AIDS Co-Morbidity in Children: Linkage of Databases from Espírito Santo State, Brazil

    PubMed Central

    Dietze, Reynaldo; Maciel, Ethel L.; Prado, Thiago N.; Caus, Antonio L.; Silva, Murilo M.; Golub, Jonathan E.

    2011-01-01

    We assessed the prevalence of AIDS among children diagnosed with active tuberculosis (TB) in Espírito Santo State, Brazil, by linking TB and AIDS surveillance databases using Reclink software and SPSS. Among 411 pediatric TB cases from 2000 to 2006, 27 (7%) were co-infected with AIDS. Most children were unable to provide a sputum specimen; co-infected patients were more likely to be smear negative for acid-fast bacilli (83% vs 46%; p = 0.07) and culture positive for Mycobacterium tuberculosis (44% vs 19%; p < 0.001). In all, 57% of co-infected patients did not react to tuberculin skin test compared with 17% of TB patients (p < 0.001). This report emphasizes the significance of AIDS in pediatric TB cases and highlights the importance of evaluating surveillance databases for gaining a better understanding of the burden of co-infection. PMID:20876684

  7. Genetic analysis of 15 autosomal and 12 Y-STR loci in the Espirito Santo State population, Brazil.

    PubMed

    Wolfgramm, Eldamária de Vargas; Silva, Beatriz Candida; Aguiar, Vitor Resende da Costa; Malta, Frederico Scott Varela; de Castro, Amanda Mafia; Ferreira, Alessandro Clayton de Souza; Prezoti, Alessandra Nunes Loureiro; de Paula, Flavia; Louro, Iúri Drumond

    2011-06-01

    This study provides population genetic data for individuals of Vitoria, Espirito Santo, Brazil, a location not yet characterized for STR frequencies used for genetic identification studies. Allelic frequencies and other population data analysis are reported for the 15 autosomal-STR loci included in the PowerPlex(®)16 kit (CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, Penta D, Penta E, TPOX, TH01 and vWA). Allele and haplotype frequencies, gene diversity and discrimination capacity were also estimated for the PowerPlex(®) Y System (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Blood samples were obtained from 226 unrelated volunteers (135 males and 91 females) residents in the city of Vitoria, representing a typical sample of the mixed ethnicity present in the Espirito Santo State, Brazil. Within the tested population, the total number of individuals typed for specific markers is: 226 for D13S317, D21S11, D3S1358, D7S820, D8S1179 and FGA; 225 for D16S539 and D5S818; 224 for D18S51; 223 for CSF1PO; 222 for Penta D and vWA; 220 for Penta E; 207 for TPOX and 142 for TH01. Y-STR haplotypes were analyzed for 102 unrelated males, being 71 of them present in the 135 autosomal-STR sample, and 31 new males tested only for Y-STR markers. All autosomal markers were in Hardy-Weinberg Equilibrium. Y-STR analysis identified 101 haplotypes, being 100 of them unique. PMID:20965142

  8. Ethical proceedings against dentists in Espírito Santo for infringements to the code of dental ethics.

    PubMed

    Santos Pacheco, Karina Tonini dos; Silva Junior, Manoelito Ferreira; Meireles, Naiara Ribeiro

    2014-01-01

    Just like any other profession, dentistry requires ethical and moral responsibilities that must be fulfilled, and the duty of every professional is to meet his obligations under the law. In light of the Código de Ética Odontológica (CEO-Code of Dental Ethics), this research aims to expound on the ethical violations committed by dentists in their practice, according to the ethical review process proposed by the Conselho Regional de Odontologia do Estado do Espírito Santo (CRO/ES-Regional Council of Dentistry of the State of Espírito Santo). The study is both retrospective and descriptive, using a quantitative approach. Data collection comprised all the ethical proceedings filed at the CRO/ES, between the years 2000 and 2011. Considering the 529 cases examined, the most frequent reason for infringement was illicit advertising (39.7%), followed by technical error (18.7%), irregular registration (16.8%), patient/professional relationship (11.3%) tax lien foreclosure (6.6%), professional/professional relationship (4.0%), irregular/illegal cover up practice (0.9%), consumer relations (0.8%), failure to provide care (0.6%), false certification (0.4%), and disrespect for authority and colleagues (0.2%). Most (59.2%) of the dental surgeons (DSs) involved were male, 35.5% were between 31 and 40 years of age, 85.8% had graduated over five (5) years prior, and 73.2% were general practitioners. Only 22 (4.2%) cases went to trial, resulting in 8 (36.4%) acquittals and 14 (63.6%) convictions. The most commonly applied penalties were private warning+pecuniary penalty (8 or 57.1%). It was concluded that the CEO must be followed with more discipline, and that professionals should seek information about their duties and obligations under the law, on behalf of patient welfare. PMID:24820427

  9. El Niño-Southern Oscillation-related salinity variations recorded in the skeletal geochemistry of a Porites coral from Espiritu Santo, Vanuatu

    NASA Astrophysics Data System (ADS)

    Kilbourne, K. Halimeda; Quinn, Terrence M.; Taylor, Frederick W.; Delcroix, Thierry; Gouriou, Yves

    2004-12-01

    Coral skeletal geochemistry offers the potential to reconstruct the sea surface salinity (SSS) history of the tropical oceans on seasonal to interannual and perhaps centennial timescales because of the strong link between variation in SSS and seawater δ18O in tropical regions. We explore this potential using a monthly resolved, 65-year record of skeletal δ18O and Sr/Ca variations in a Porites coral from Espiritu Santo, Vanuatu. We demonstrate that El Niño-Southern Oscillation-related climate variability strongly influences coral δ18O at Santo through local salinity changes associated with the position of the South Pacific Convergence Zone and the movement of its associated salinity front. Such a demonstration provides the "ground truth" data that can be used to place paleoclimate variability estimated using existing fossil coral records from this region into a modern conceptual framework. We also evaluate different methods of combining coral δ18O and Sr/Ca to reconstruct SSS and conclude that the coral δ18O anomaly time series provides the best fit to recent in situ SSS data at Santo.

  10. Stratigraphy of the Pleistocene, phonolitic Cão Grande Formation on Santo Antão, Cape Verde

    NASA Astrophysics Data System (ADS)

    Eisele, S.; Freundt, A.; Kutterolf, S.; Ramalho, R. S.; Kwasnitschka, T.; Wang, K.-L.; Hemming, S. R.

    2015-08-01

    The Cão Grande Formation (CGF) on the western plateau of Santo Antão Island is part of the younger volcanic sequence that originated from both, basanitic and nephelinitic magmatic suites, respectively called COVA and COROA suites. Based on our detailed revised stratigraphy of the CGF, including two yet unknown tephra units, we can show that both suites produced multiple, highly differentiated eruptions over a contemporaneous period. Correlations of CGF tephras with marine ash layers provide distal dispersal data for Cão Grande I (CG I) and also identify two highly explosive, phonolitic eruptions that pre-date the CGF tephra deposits known on land. Within the CGF, the lowermost, 220 ± 7 ka old unit Canudo Tephra (CT; COVA suite) comprises phonolitic fall deposits and ignimbrites; it is partly eroded and overlain by debris flow deposits marking a hiatus in highly differentiated eruptions. The phonolitic CG I Tephra (COROA suite) consists of an initial major Plinian fall deposit and associated ignimbrite and terminal surge deposits. This is immediately overlain by the phonolitic to phono-tephritic Cão Grande II (CG II; COVA suite), a complex succession of numerous fallout layers and density-current deposits. CG I and CG II have radiometric ages of 106 ± 3 ka and 107 ± 15 ka, respectively, that are identical within their error limits. The youngest CGF unit, the Furninha Tephra (FT; COROA suite), consists of three foidic-phonolitic fall deposits interbedded with proximal scoria deposits from a different vent. The phonolitic eruptions switched to and fro between both magmatic suites, in each case with a stronger first followed by a weaker second eruption. Each eruption evolved from stable to unstable eruption columns. During their terminal phases, both magma systems also leaked evolved dome-forming lavas next to the tephras. Distal ashes increase the CG I tephra volume to ~ 10 km3, about twice the previously published estimate. The tephra volume of CG II is ~ 3 km

  11. The long-living Cao Grande magmatic system on Santo Antão, Cape Verde Islands - A revised model

    NASA Astrophysics Data System (ADS)

    Eisele, S.; Freundt, A.; Kutterolf, S.; Ramalho, R. S.

    2013-12-01

    Santo Antão represents an ocean island shield volcano in its early post-erosional stage. At this stage, mafic Strombolian volcanic activity had been replaced by Pleistocene highly explosive phonolitic eruptions. Two phonolitic tephra successions, Cão Grande Pumice units I and II (CG I and CG II), were previously identified and described (Holm et al. 2006; Mortensen et al. 2009). Our tephrostratigraphic fieldwork on Santo Antão identified a third older explosive phonolitic phase (CG 0) and two phonolitic domes. While CG 0 and CG I are separated by an erosional unconformity, CG II succeeds directly on CG I. This is also assisted by our preliminary 40Ar/39Ar ages that overlap for CG I and CG II and indicate a break in eruptive history of c. 40 ka between CG 0 (206 × 8 ka) and CG I (163 × 15 ka). Based on XRF bulk rock analyses Holm et al. (2006) assigned CG I and CG II to two discrete magmatic series, a nephelinite - phonolite trend and a basanite - phonolite trend respectively. According to this, our data shows that the older CG 0 is also related to the basanite - phonolite series. Although field observations and geochronology indicate that CG I and CG II erupted in rapid succession from the same area around the Monte Figueira dome, there is no evidence for direct interaction of both magmatic systems. Using major and trace element glass compositions we can show that all three units have distinct geochemical compositions but the initial stage of CG II can be related compositionally to CG 0. This suggests that both units derived from the same magmatic system, which is also supported by thermobarometry data displaying similar temperatures and pressures around 970 °C and 900 MPa for CG 0 and CG II and significant lower values (900 °C; 500 MPa) for CG I. Detailed stratigraphic linked glass analyses, representing the latest stage of magma evolution, show uniform compositions for CG I and therefore question the established magma chamber model of CG I from Mortensen

  12. Modern sedimentation processes in a wave-dominated coastal embayment: Espírito Santo Bay, southeast Brazil

    NASA Astrophysics Data System (ADS)

    Bastos, Alex Cardoso; Costa Moscon, Daphnne Moraes; Carmo, Dannilo; Neto, José Antonio Baptista; da Silva Quaresma, Valéria

    2015-02-01

    Sediment dynamics in wave-dominated coastal embayments are generally controlled by seasonal meteorological conditions, storms having a particularly strong influence. In the present study, such hydrodynamic processes and associated deposits have been investigated in a coastal embayment located along the southeast coast of Brazil, i.e. Espírito Santo Bay, in the winter (June/July) of 2008. The bay has undergone a series of human interventions that have altered the local hydrodynamic processes and, consequently, the sediment transport patterns. Facies distribution and sediment dynamics were examined by acoustic seabed mapping, sediment and core sampling, hydrodynamic measurements and sand transport modelling. The results show that sediment distribution can be described in terms of nearshore and offshore zones. The offshore bay sector is predominantly composed of "palimpsest" lithoclastic medium-coarse sands deposited in the course of the early Holocene transgression that peaked about 5,000 years ago. In the inner bay or nearshore zone (up to depths of 4-8 m), these older transgressive deposits are today overlain by a thin (up to 30-cm-thick) and partly patchy blanket of younger regressive fine sand/muddy fine sands. Both coarse- and fine-grained facies are being reworked during high-energy events (Hs>1.5 m) when fine sediment is resuspended, weak tide-induced drift currents causing the sand patches to be displaced. The coarser sediment, by contrast, is mobilized as bedload to produce wave ripples with spacings of up to 1.2 m. These processes lead to a sharp spatial delimitation between a fine sand/mud facies and a rippled coarse sand facies. The fine sand patches have a relief of about 20-30 cm and reveal a typical internal tempestite depositional sequence. Fair-weather wave-induced sediment transport (Hs<1 m), supported by weak tidal currents, seems to only affect the fine sediment facies. Sediment dynamics in Espírito Santo Bay is thus essentially controlled by

  13. Evolution of the Phreatomagmatic Cova de Paul Eruption, Santo Antao, Cape Verde Islands: Links Between Eruption Development and Crater Growth

    NASA Astrophysics Data System (ADS)

    Tarff, R.; Day, S. J.; Downes, H.; Seghedi, I.

    2013-12-01

    Episodes of phreatomagmatic explosive activity that excavate large deep craters occur at high-elevation vents on many oceanic island volcanoes. The water driving these explosions is sourced from freshwater aquifers within the volcanic edifices, whose location and other characteristics will influence crater growth and final geometry. Here we describe phreatomagmatic deposits from the single eruption that formed the large Cova de Paul crater on the island of Santo Antao, Cape Verde Islands. These deposits, emplaced after an initial Strombolian phase of activity, record wide fluctuations in the intensity of phreatomagmatic explosivity. The largest explosions produced low-temperature, indurated lithic-rich phreatomagmatic ignimbrites and surge deposits; these occur as isolated and often erosive-based units in more distal areas but are interbedded in proximal outcrops with airfall to mixed fall and flow breccia and ash beds containing varying proportions of lithic and juvenile clasts, pointing to a series of climactic explosions within an extended period of milder phreatomagmatic explosive activity. We find that whereas the lithic clasts in the mixed units can be matched with the varied rock units in the exposed crater walls, the ignimbrite and surge units also contain hydrothermally altered clasts that appear to have originated from deeper in the volcanic edifice. They imply that during the climactic explosions the crater was excavated to several hundred meters depth below the surface. The phreatomagmatic explosive phase of the CDPC eruption was initiated by shattering of the chilled margins of the eruptive conduit, which had formed during the early Strombolian activity. The remains of these chilled margins form a distinctive bed of large flow-banded angular sub-glassy juvenile clasts that separates the Strombolian deposits from the later phreatomagmatic deposits. At this point water inflow from pressurized aquifers into the conduit attained a critical coolant input

  14. Weapons of mass destruction preparedness and response for the XIV Pan-American Games, Santo Domingo, 2003.

    PubMed

    Báez, Amado Alejandro; Sztajnkrycer, Matthew D; Giráldez, Ediza M; Comprés, Hugo Pérez

    2006-01-01

    The Pan-American Games are considered to be the fourth most important international athletic event in the world. Hosted by the city of Santo Domingo, Dominican Republic, the XIV Pan-American Games lasted from 11-17 August 2003. In preparation for the Games, the Security Directorate developed and deployed a Weapons of Mass Destruction (WMD) Unit. For operational support, two strike teams (Alpha and Bravo) were active at any given time. Each team consisted of five members including a team leader, field physician, explosive ordinance disposal (EOD) officer, and two tactical officers. Three hospitals-two military and one civilian--were designated as primary medical centers for the event. With the assistance of the WMD Unit, emergency department staff were trained in the medical management of a WMD event, response protocols for WMD were created, and special decontamination areas were designated. Syndromic surveillance was performed by means of direct communications between the hospitals and units, as well as use of an electronic, Web-based surveillance tool. PMID:17076426

  15. [The genera of Bethylidae (Hymenoptera: Chrysidoidea) in four areas of Atlantic Rain Forest from Espírito Santo, Brazil].

    PubMed

    Mugrabi, Daniele F; Alencar, Isabel D C C; Barreto, Francisco C C; Azevedo, Celso O

    2008-01-01

    The generic richness and abundance of Bethylidae collected in four different hillside areas of Atlantic rain forest from Espírito Santo, Brazil were studied. The sites are Santa Maria de Jetibá (SMJ), Domingos Martins (DM), Pancas (P) and Atílio Vivacqua (AV). A total of 2,840 specimens of 12 genera were collected. Lepidosternopsis Ogloblin and Bakeriella Kieffer are first recorded from the State. Richness of taxa was calculated using first-order Jackknife richness with EstimateS program. Genera accumulation curves were ran to evaluate the samples. Abundance data were adjusted to the geometric distribution. Parameter k was used to compare areas. The generic profile was not equal for the sites we studied. The areas were considered disturbed. SMJ and DM presented genera richness bigger than in P and AV. The differences in the sites reflect the different preservation of each environment. Pseudisobrachium Kieffer and Dissomphalus Ashmead are most dominant genera in SMJ, DM and P, and Anisepyris Kieffer in AV. This study emphasizes the fact of Dissomphalus as the most abundant genus in rain forests. The generic profile found in AV is similar to that of some areas of Brazilian savannah. PMID:18506293

  16. Individual determinants of fish choosing in open-air street markets from Santo André, SP/Brazil.

    PubMed

    Vasconcellos, Juliana Parreira; Vasconcellos, Silvio Arruda; Pinheiro, Sonia Regina; de Oliveira, Thaís Helena Nishikata; Ribeiro, Naassom Almeida Souza; Martins, Cassia Neves; Porfírio, Bruno Augusti; Sanches, Sandra Abelardo; de Souza, Orlando Bispo; Telles, Evelise Oliveira; Balian, Simone de Carvalho

    2013-09-01

    The objective of this study was to identify the determinants of fish consumption in the population that attends open-air street markets in the city of Santo André, SP, Brazil.We performed a survey, covering approximately 482 people in 49 street markets.It consisted of free-answer questions, half open choice and half multiple-choice options, for the identification and evaluation of socioeconomic factors that facilitate and hinder fish consumption.A descriptive analysis of the data and further tests were used to determine the association between variables and linearity with consumption, with a significance level of 5%. The most commonly cited types of fish consumed were hake, sardine and dogfish. The factors that facilitate the purchase and consumption of fish are listed as follows: a preference for purchasing fish at street markets, appearance, firmness, fresh presentation, frozen presentation, as well as the respondent's education and individual monthly income. Limiting factors were identified as the price and the presence of spines. Perishability, odour, ethnicity, proximity to points of sale of residence and work, gender, age, number of people in the household, presence of children and acquisition supermarket were not characteristics that influenced decisions about fish consumption. PMID:23643568

  17. All different, all equal: Evidence of a heterogeneous Neolithic population at the Bom Santo Cave necropolis (Portugal).

    PubMed

    Gonçalves, D; Granja, R; Alves-Cardoso, F; Carvalho, A F

    2016-06-01

    The objective of this paper was to contribute to the discussion regarding the socio-political organization of south-western Iberian Middle Neolithic populations. To that end, the preservation and distribution of human remains and the dispersion of grave goods within two rooms of the Bom Santo Cave (Rooms A and B) were investigated and combined with genetic and isotopic data previously published. Grave goods distribution and skeletal analyses highlighted an important diversity in terms of funerary practices thus corroborating data from ancient DNA and Sr/O isotopic analyses that suggested a great genetic and geographic diversity. Grave goods presented an uneven spatial distribution and were made of raw materials from different sources and using different pottery manufacturing styles albeit typologically homogeneous. The preservation and distribution of human remains suggested that Room A was mainly used for secondary depositions while Room B was used for both primary and secondary depositions. No link between the two rooms was found since remains from the same individuals were apparently exclusive of one room or another. The results suggest that this society presented substantial inner genetic, social and geographical heterogeneity. Most probably, this was due to the presence of distinct but coeval groups in the cave that shared a larger-scale social identity (as in "segmentary societies") or, less likely, to the presence of one single, but internally heterogeneous society (as in fully sedentary societies) that assimilated foreigners. PMID:27016326

  18. Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador

    PubMed Central

    Rodríguez-Hidalgo, Richar Ivan; Contreras-Zamora, Javier; Benitez Ortiz, Washington; Guerrero-Viracocha, Karina; Salcan-Guaman, Holger; Minda, Elizabeth; Ron Garrido, Lenin

    2015-01-01

    The Province of Santo Domingo de los Tsáchilas in Ecuador represents the largest informal cattle market. Because of its strategic position, cattle movement is very high and therefore we selected this region, to determine the strain variation of Brucella sp. Part of the study aimed at the isolation, biotyping, and genotyping of Brucella species from milk and supra-mammary lymph nodes of sero-positive bovines, using selective Farrell medium, biochemical assays, and IS711-PCR, AMOS-PCR, and HOOF-Prints techniques. In total, 656 animals from 12 sero-positive dairy herds and from the provincial slaughterhouse were diagnosed by Rose Bengal and Wright’s Slow Agglutination test with EDTA. Amongst these animals, 50 animals were sero-positive for brucellosis. Twenty-five lymph nodes and 25 milk samples from each group of positive reactors were transferred to culture medium. Isolation was possible from 4 (16%) lymph nodes and 9 (36%) milk samples; out of these, 10 isolates were diagnosed as Brucella sp. All four isolates of lymphatic tissue corresponded to Brucella abortus biotype 1, confirmed as field strains by molecular analysis. Milk isolations, showed biochemically a more dispersed pattern in which B. abortus biotypes 1 and 4 were found; yet four samples gave a pattern similar to B. abortus biotype 2; however, only biotypes 1 and 4 were confirmed by molecular analysis. The concordance between biochemical and molecular diagnostic tests reached 76.9%. PMID:25806363

  19. Baseline trace elements in the seagrass Halodule wrightii Aschers (Cymodoceaceae) from Todos os Santos Bay, Bahia, Brazil.

    PubMed

    Brito, Geysa B; de Souza, Thaís L; do N Costa, Fernanda; Moura, Carlos W N; Korn, Maria Graças A

    2016-03-15

    Trace elements concentrations (As, Ba, Cd, Cr, Cu, Ni, Pb, V and Zn) were found in samples of Halodule wrightii Aschers (Cymodoceaceae) seagrass from Todos os Santos Bay, Bahia, Brazil by inductively coupled plasma mass spectrometry (ICP-MS). This bay has been impacted by the presence of industrial activity that includes chemical and petrochemical plants as well as an oil refinery and harbor activities located in the north and northeastern area. Samples were collected at 4 stations to evaluate the distribution of element concentrations in this area and under different climatic conditions. The mean concentrations, in mgkg(-1), found in dry tissues for all the studied metals and stations were: As (1.08-5.42), Ba (3.72-32.0), Cd (0.135-1.68), Cr (1.15-10.4), Cu (2.23-13.4), Ni (1.95-9.87), Pb (0.873-5.18), V (3.39-22.4) and Zn (13.1-39.5). Statistical analysis (ANOVA) of the seagrass data showed significant inter-site differences for all elements examined except nickel and vanadium. PMID:26874749

  20. Prevalence and risk factors associated with endoparasitosis of dogs and cats in Espírito Santo, Brazil.

    PubMed

    Campos, Diefrey Ribeiro; Oliveira, Luanna Castro; de Siqueira, Daniele Fassina; Perin, Lívia Reisen; Campos, Nayara Camatta; Aptekmann, Karina Preising; Martins, Isabella Vilhena Freire

    2016-09-01

    The objective of this study was to determine the prevalence and risk factors for the occurrence of endoparasitosis in dogs and cats in Espírito Santo, Brazil. For the study, 345 dogs and 160 cats were examined. Faecal samples from the animals were collected, and owners were interviewed about their handling of their animals. For the diagnosis of the infections, the Willis-Molley, simple centrifugal flotation and formalin-ether sedimentation techniques were performed. The data found in the tests were tabulated and analysed with a chi-square test (p <0.05), and calculation of odds ratios (OR) with confidence intervals of 95% were then performed to determine the association between the variables and the outcome of stool examinations. The prevalence of parasites was 59% for dogs and 54% for cats. The genus Ancylostoma was the most prevalent (45%). Supplying filtered water, not feeding raw foods, standardisation of a defecation site and cleaning up the faeces reduced the chances of developing intestinal parasites. An increased frequency of deworming was also shown to be a protective factor; specifically animals which received anthelmintic more than once a year had a lower predisposition for intestinal parasites. PMID:27447218

  1. Geochemical and biostratigraphic characterization of an upper Cretaceous organic-rich condensed section in the Santos Basin, Brazil

    SciTech Connect

    Trindade, L.A.F., Porsche, E.; Penteado, H.L.B. )

    1996-01-01

    The Cenomanian-Turonian section is worldwide recognized as an organic- rich interval associated with a widespread anoxic event. Samples from this interval, representing proximal sedimentary conditions have been well characterized in the Santos Basin, Brazil, where it reaches 300m thickness. The main objective of this study is to characterize the geochemical and paleoecological attributes as well as to ascertain the biostratigraphic position of this organic-rich section in a more distal location, at ODP Site 356. Biostratigraphic analyses identify die interval at ODP Site 356 as a condensed section ranging in age from Cenomanian to Coniacian (11 m), deposited under anoxic conditions associated with a warm, and climate in the adjacent continental areas. Geochemical analyses indicate high total organic carbon contents (up tp 6.7%), dominance of algal derived material, high relative abundances of homohopanes and methylsteranes, [delta][sup 13]C around -27[per thousand], indicating a typical marine anoxic section. Despite the high content of oil-prone organic matter, this section has not attained thermal evolution appropriated for hydrocarbon generation due to its shallow burial. In proximal areas of the basin, this section is interpreted as a transgressive system tract which is composed by a series of sequences, that are enriched in organic material at their bases. Basinward the interval is thinner, but presents higher organic carbon contents according to geochemical data of ODP Site 356, typical of a condensed section.

  2. Geochemical and biostratigraphic characterization of an upper Cretaceous organic-rich condensed section in the Santos Basin, Brazil

    SciTech Connect

    Trindade, L.A.F., Porsche, E.; Penteado, H.L.B.

    1996-12-31

    The Cenomanian-Turonian section is worldwide recognized as an organic- rich interval associated with a widespread anoxic event. Samples from this interval, representing proximal sedimentary conditions have been well characterized in the Santos Basin, Brazil, where it reaches 300m thickness. The main objective of this study is to characterize the geochemical and paleoecological attributes as well as to ascertain the biostratigraphic position of this organic-rich section in a more distal location, at ODP Site 356. Biostratigraphic analyses identify die interval at ODP Site 356 as a condensed section ranging in age from Cenomanian to Coniacian (11 m), deposited under anoxic conditions associated with a warm, and climate in the adjacent continental areas. Geochemical analyses indicate high total organic carbon contents (up tp 6.7%), dominance of algal derived material, high relative abundances of homohopanes and methylsteranes, {delta}{sup 13}C around -27{per_thousand}, indicating a typical marine anoxic section. Despite the high content of oil-prone organic matter, this section has not attained thermal evolution appropriated for hydrocarbon generation due to its shallow burial. In proximal areas of the basin, this section is interpreted as a transgressive system tract which is composed by a series of sequences, that are enriched in organic material at their bases. Basinward the interval is thinner, but presents higher organic carbon contents according to geochemical data of ODP Site 356, typical of a condensed section.

  3. Rovearinids (stemless crinoids) in the Albian carbonates of the offshore Santos Basin, southeastern Brazil: stratigraphic, palaeobiogeographic and palaeoceanographic significance

    NASA Astrophysics Data System (ADS)

    Dias-Brito, D.; Ferré, B.

    2001-06-01

    Microfacies analysis of marine carbonates cored by Petrobras well 1-SPS-6 in the offshore Santos Basin (southeastern Brazil) has revealed a remarkable fossil assemblage of calpionellids (colomiellids), favusellids, hedbergellids, globigerinelloidids, buliminids, radiolarians, inoceramid prisms, roveacrinids, and saccocomids(?) preserved in lower Albian calcimudstones-wackestones of the lower part of the Guarujá Formation. This assemblage represents an allochtonous accumulation in a deep neritic to shallow bathyal hypoxic environment. Besides 'saccocomid-like' sections, the only determinable sections of roveacrinids are thecal plates of Poecilocrinus dispandus elongatus Peck, 1943. This species was previously only known from the Weno Formation of Texas. The Brazilian material extends its records farther south from at least the lower Albian, which then represents the earliest occurrence of this peculiar family in the South Atlantic region. Taking into account their Albian global distribution and the location of their oldest representative (Hauterivian near Alicante, Spain), the Roveacrinidae dispersed westward throughout all of Cretaceous Tethys. The Tethyan origin of Roveacrinidae is further evidence that, during late Aptian-Albian times, the northern South Atlantic (north of the Walvis-São Paulo Ridge) was supplied by a Tethyan water mass.

  4. Applying GIS to develop a model for forest fire risk: A case study in Espírito Santo, Brazil.

    PubMed

    Eugenio, Fernando Coelho; dos Santos, Alexandre Rosa; Fiedler, Nilton Cesar; Ribeiro, Guido Assunção; da Silva, Aderbal Gomes; dos Santos, Áureo Banhos; Paneto, Greiciane Gaburro; Schettino, Vitor Roberto

    2016-05-15

    A forest fire risk map is a basic element for planning and protecting forested areas. The main goal of this study was to develop a statistical model for preparing a forest fire risk map using GIS. Such model is based on assigning weights to nine variables divided into two classes: physical factors of the site (terrain slope, land-use/occupation, proximity to roads, terrain orientation, and altitude) and climatic factors (precipitation, temperature, water deficit, and evapotranspiration). In regions where the climate is different from the conditions of this study, the model will require an adjustment of the variables weights according to the local climate. The study area, Espírito Santo State, exhibited approximately 3.81% low risk, 21.18% moderate risk, 30.10% high risk, 41.50% very high risk, and 3.40% extreme risk of forest fire. The areas classified as high risk, very high and extreme, contemplated a total of 78.92% of heat spots. PMID:26974239

  5. Lysosomal responses as a diagnostic tool for the detection of chronic petroleum pollution at Todos os Santos Bay, Brazil.

    PubMed

    Martins, L K P; Nascimento, I A; Fillmann, G; King, R; Evangelista, A J A; Readman, J W; Depledge, M H

    2005-11-01

    Coastal marine environments, especially semienclosed systems such as bays, are under unrelenting stress caused by urban and industrial development. Biomonitoring plays a vital role in strategies to identify, assess, and control stressors. However, due to the magnitude of the challenge there is a demand for new and innovative approaches to provide timely and accessible information to environmental managers and policy makers. The present work aimed to assess hydrocarbon levels in sediments from petroleum-related industrial areas at Todos os Santos Bay (Brazil) and associate them to toxicity-induced responses (neutral red retention (NRR) assay) by the burrowing clam Anomalocardia brasiliana. Surface sediments collected during the dry and rainy seasons were analyzed for aliphatic and aromatic hydrocarbons. At the control site, hydrocarbon levels were low and mainly biogenic. The aliphatic hydrocarbon ("total unresolved complex mixture," alkanes, and isoprenoids) concentrations indicated a chronic situation with very little "fresh" oil contamination at the oil-related sites. The polycyclic aromatic hydrocarbons indicated sites moderately contaminated by chronic oil and some pyrolytic input. The effects of those contaminants were assessed by the lysosomal NRR assay applied to A. brasiliana hemocytes. Sediment toxicity at the oil-related sites was evidenced by the lowered capacity of the lysosomes to retain the neutral red dye compared to results from the control site. This research indicates that the NRR assay is a useful and efficient screening technique able to discriminate polluted from clean sites. PMID:16307981

  6. A numerical modeling analysis of the phytoplankton and nutrients dynamics for Todos Santos Bay and northwestern Baja California

    NASA Astrophysics Data System (ADS)

    Cruz Rico, J. E., Sr.; Rivas, D.

    2015-12-01

    A tridimensional physical-biological numerical model is implemented for the Todos Santos Bay and the northwest of Baja California to investigate the mechanics and ecological processes associated with the regional plankton dynamics. An NPZD (Nitrate, Phytoplankton, Zooplankton, and Detritus) ecosystem simple model is used to describe the distribution and evolution of the lower trophic levels in the area of study. The model adequately reproduces the spatial distribution of the concentration of chlorophyll for the different seasons of the year. In general, the distribution of the subsurface chlorophyll maximum (SCM) depends primarily on the seasonal circulation patterns, the total solar irradiance, and the vertical flux of nutrients. Interannual variability shows two extreme years in the analyzed period: 2006 and 2007. Year 2006 was an anomalous warm year, with a weak upwelling activity and low chlorophyll concentrations compared to year 2011. These anomalies are related to the activity of the Pacific Decadal Oscillation, the El Niño+3, and the regional Outgoing Longwave Radiation. Thus, in spite of the simplicity of the NPZD model, both temporal and spatial patterns of distribution of chlorophyll and nutrients are generally reproduced.

  7. Seismic Site Characterization through Joint Modeling of Complementary Data Functionals, with Applications to Santo Domingo, Dominican Republic

    NASA Astrophysics Data System (ADS)

    Schwed, M.; Pulliam, J.; Sen, M. K.; Willemann, R. J.; Huerta-Lopez, C.; Moschetti, M. P.; Schmitz, M.; Louie, J. N.; Polanco, E.; Huerfano Moreno, V.; Pasyanos, M.

    2013-12-01

    New approaches suggest that it may be possible to determine ground shaking during earthquakes through low-cost, non-invasive seismic surveys that make use of ambient noise, and that the results can be used for 'shake-casting' to produce scenarios for the purposes of urban planning, improving community resilience, and emergency response. We will present a strategy for determining seismic 'site characterization' through joint modeling of and horizontal to vertical spectral ratios (HVSR) and surface wave dispersion, determined via spatial autocorrelation (SPAC), refraction microtremor (ReMi), and/or multi-channel analysis of surface waves (MASW). Fitting of data functionals by synthetics is driven by global optimization and the models are assessed quantitatively. The products of this approach are shear wave velocity profiles for the shallow subsurface, accompanied by posterior probability distributions and parameter correlation matrices that allow for the assessment of model reliability. Optimization strategies for solving nonlinear problems in geophysics have several advantages over linearized inversions. Jointly fitting dispersion curves and HVSR functionals via global optimization allows us to characterize the space of possible models, assess model reliability, identify parts of the 'best-fit' model that are poorly constrained, and guide us toward new data that might improve constraints on the model. Tools such as the posterior probability distribution and the parameter correlation matrix allow us to assess the relative contribution of both types of data to model constraints and how to choose the optimal weights between data types. The joint modeling technique is applied to data acquired in an NSF-funded Pan-American Advanced Studies Institute in Santo Domingo, Dominican Republic, entitled 'New Frontiers in Geophysical Research: Bringing New Tools and Techniques to Bear on Earthquake Hazard Analysis and Mitigation', as a proof-of-concept survey in a highly built

  8. Climate patterns governing the presence and permanence of salmonellae in coastal areas of Bahia de Todos Santos, Mexico.

    PubMed

    Simental, Lourdes; Martinez-Urtaza, Jaime

    2008-10-01

    Despite the importance of salmonellae as one of the major causes of food-borne infections worldwide, data regarding the presence of these organisms in the environment are limited. We investigated the presence of Salmonella spp. in Bahia de Todos Santos (Baja California, Mexico) and evaluated the environmental factors that affect the occurrence of Salmonella spp. in this arid region. A total of 1,331 samples collected from 21 sites along the coast during a period of 3 years were analyzed for Salmonella spp. Geographical and seasonal distribution of Salmonella spp. was evaluated in association with environmental parameters and with human infections in the area. The incidence of Salmonella bacteria throughout the study was 4.8%, with the highest incidence detected in wastewater (16.2%), followed by stream water (10.6%), mollusks (7.4%), and seawater (2.3%). Twenty different serotypes were identified among the 64 Salmonella isolates. The dominant serotype was Typhimurium (23.4%), followed by Vejle (6.2%). The presence of Salmonella spp. in coastal areas was mostly confined to rainy periods and areas of stream discharges, and runoff was identified as the predominant factor influencing the transport of Salmonella bacteria from source points to the sea via streams. Isolation of Salmonella spp. was negatively and significantly associated with temperature, probably because of the effect of solar radiation in the decline of permanence of Salmonella bacteria. Conversely, human infections prevailed during the warmest months and were negatively correlated with the presence of Salmonella spp. in the marine environment. PMID:18708509

  9. Salt roller growth as a controlling factors of the Albian rafts on Espírito Santo Basin, SE Brazil.

    NASA Astrophysics Data System (ADS)

    Piedade, Aldina; Alves, Tiago

    2014-05-01

    The SE Brazil continental margin is dominated by continental-slope embankment, incision of submarine channel system and significant mass-gravitational processes, with salt tectonics playing a significant role in upper-crust deformation. It is known to comprise a margin rich in oil and gas, in which raft tectonics plays an important role in their migration and accumulation. A combination of 3D pre-stack and post-stack time-migrated (PSTM) data was used to interpret the evolution of the Albian rafts in Espírito Santo Basin, SE Brazil was used in this work to: a) investigate the impact of continuing halokinesis in raft movement and, b) critically assess the parameters considered to control raft tectonics. In the study area Albian rafts, which are laterally constrained by salt rollers that were reactivated during the Late Cretaceous-Cenozoic, are seen to be ramped over triangular salt structures, and rotated by growing salt pillows. As a result, we observe complex compartmentalization styles in the rafts, with three main types of faults being recognised in four distinct rafts: a) rollover faults formed axially to large N-S rafts, b) radial faults to segments of rafts that record torsion and enhanced downslope movement, c) reactivated crestal and keystone faults formed on the flanks of growing salt diapirs onto which rafts are ramped upon. The space between the rafts was also analysed and quantified and show to be influenced by the weight of the sediments overburden. This work shows that slope gradient and overburden thickness had little control on raft segmentation. We interpret these data as a proof that underlying salt was a primary control on raft tectonics in the study area, overriding the effect of overburden thickness and variable slope gradients on the rafts' internal compartmentalisation. This study will show different styles of compartmentalization observed in Albian rafts from SE Brazil.

  10. Geological setting and paleomagnetism of the Eocene red beds of Laguna Brava Formation (Quebrada Santo Domingo, northwestern Argentina)

    NASA Astrophysics Data System (ADS)

    Vizán, H.; Geuna, S.; Melchor, R.; Bellosi, E. S.; Lagorio, S. L.; Vásquez, C.; Japas, M. S.; Ré, G.; Do Campo, M.

    2013-01-01

    The red bed succession cropping out in the Quebrada Santo Domingo in northwestern Argentina had been for long considered as Upper Triassic-Lower Jurassic in age based on weak radiometric and paleontological evidence. Preliminary paleomagnetic data confirmed the age and opened questions about the nature of fossil footprints with avian features discovered in the section. Recently the stratigraphic scheme was reviewed with the identification of previously unrecognized discontinuities, and a radiometric dating obtained in a tuff, indicated an Eocene age for the Laguna Brava Formation and the fossil bird footprints, much younger than the previously assigned. We present a detailed paleomagnetic study interpreted within a regional tectonic and stratigraphic framework, looking for an explanation for the misinterpretation of the preliminary paleomagnetic data. The characteristic remanent magnetizations pass a tilt test and a reversal test. The main magnetic carrier is interpreted to be low Ti titanomagnetites and to a lesser extent hematite. The characteristic remanent magnetization would be essentially detrital. The obtained paleomagnetic pole (PP) for the Laguna Brava Formation has the following geographic coordinates and statistical parameters: N = 29, Lon. = 184.5° E, Lat. = 75.0° S, A95 = 5.6° and K = 23.7. When this PP is compared with another one with similar age obtained in an undeformed area, a declination anomaly is recognized. This anomaly can be interpreted as Laguna Brava Formation belonging to a structural block that rotated about 16° clockwise along a vertical axis after about 34 Ma. This block rotation is consistent with the regional tectonic framework, and would have caused the fortuitous coincidence of the PP with Early Jurassic poles. According to the interpreted magnetostratigraphic correlation, the Laguna Brava Formation would have been deposited during the Late Eocene with a mean sedimentation rate of about 1.4 cm per thousand years, probably in

  11. Prevalence of liver diseases as referred by people living in the Santos and São Vicente Estuary.

    PubMed

    Carvalho, Daniele Fernandes Pena; Guimarães, M T; Ribeiro, T S; Campina, N N; Lobarinhas, M R; Lopes, A L J; Cunha, M G; Souza, I B; Oliveira, V L F; Braga E Braga, L; Martins, L C; Gomes, A; Pereira, L A A; Braga, A L F

    2015-10-01

    The Santos and São Vicente Estuary has suffered extensively over the years from irregular industrial deposits. The present study aimed to evaluate liver disease prevalence and potential associated risk factors in four of the Estuary's areas (Pilões and Água-Fria, Cubatão Center, Continental São Vicente, and Guarujá) and a reference area (Bertioga). This study consisted of a cross-sectional study design, in which a questionnaire was used to collect information in 820 households at each of the study areas. The proportion of total liver diseases, hepatitis, cirrhosis, and cancer (liver, biliary tract, and pancreas) per area were estimated. Pearson's chi-square test and two proportion differences test were applied in order to evaluate associations between disease occurrence and areas and to test differences between two proportions, respectively. Single and multiple logistic regression models were applied to assess associations between disease prevalence and the different study areas. Liver disease prevalence was 1.5 % among all inhabitants and 1.4 % among those without any type of exposure. Among those who reported the presence of liver disease, a higher percentage of the participants that reported hepatitis (27.7 %) or other liver disease (48.7 %) did not report occupational or alcohol exposures. Hepatitis (77.8 %) was the most reported disease, and a statistical association between living in Pilões and Água-Fria and the occurrence of hepatitis was observed (Pearson's χ (2): z = 18.1; p = 0.001). The consumption of locally-produced groceries (2.88; CI: 1.24-6.70) and water (5.88; CI: 2.24-15.45) were shown to be risk factors for the occurrence of liver disease. Thus, environmental exposure is still a public health problem present in the estuary region. PMID:24870289

  12. Constraining Basin Geometry and Fault Kinematics on the Santo Tomas Segment of the Agua Blanca Fault Through a Combined Geophysical and Structural Study

    NASA Astrophysics Data System (ADS)

    Springer, A.; Wetmore, P.; Fletcher, J.; Connor, C. B.; Callihan, S.; Beeson, J.; Wilson, J.

    2008-12-01

    The Santo Tomas basin, located in northern Baja California, formed at a right step in the dextral Agua Blanca fault (ABF). The ABF extends for more than 120km east from Punta Banda, with an east-west strike, and represents the southernmost fault in the San Andreas system of faulting. The basin is located roughly 40km south of Ensenada where the Agua Blanca fault intersects the Maximos fault. A detailed geophysical analysis defines the basin geometry, and helps to constrain the distribution and offset of mapped and concealed faults. Geophysical and structural data sets are combined to constrain the kinematic evolution of the Santo Tomas basin, including determining the relative amount of dip-slip and strike-slip motion on basin-bounding faults. Gravity data was collected over seven transects across and along the axis of the basin at 500 meter intervals, with 200 meter intervals at locations of known or inferred faults. Magnetic data were taken over the same lines, and are used in conjunction with gravity data to constrain the locations, geometries and displacements of intrabasinal faults. The combined gravity and magnetic data are modeled using Geosoft Oasis montaj software to create 2 3/4D models along profiles across the study area. Modeling of the geophysical data combined with structural mapping indicates that the Santo Tomas basin is bound by two major strike-slip faults, the ABF on the northeastern side and the Maximos fault on south, Based on offset markers, most of the strike-slip motion appears to be concentrated on the ABF on the north side of the basin. The ABF fault is characterized by multiple subparallel fault strands that appear to coalesce into single strands to the northwest and southeast of the basin. The Maximos is characterized by a single strand throughout the basin and it exhibits a minor dip-slip component. Basin sediments thicken slightly against the Maximos fault to as much as 1km. A third fault, cutting across the basin southeast of the

  13. A new turtle from the Upper Cretaceous Bauru Group of Brazil, updated phylogeny and implications for age of the Santo Anastácio Formation

    NASA Astrophysics Data System (ADS)

    Menegazzo, Mirian Costa; Bertini, Reinaldo José; Manzini, Flávio Fernando

    2015-03-01

    A new Podocnemidinura specimen from the Upper Cretaceous Bauru Group (Paraná Basin) of southeastern Brazil was described. The Bauru Group provided an important portrait of the Brazilian Mesozoic terrestrial biota, which boasts a vertebrate fauna formed from fishes, frogs, lacertilians, crocodyliforms, dinosaurs and mammals; records of palynomorphs; and invertebrate fauna consisted of gastropods, bivalves, ostracods and conchostracans. Nevertheless, the age of these continental deposits is not precisely estimated, which prevents global correlations, and its fauna is argued to be endemic. The new specimen described is the first turtle from the Santo Anastácio Formation, and its morphological comparison with other South American forms provided a significant advancement in the understanding of the age of this unit (Late Cretaceous). This study permitted a revision of the turtle taxa of the Bauru Group. As a result, some taxa were considered synonym, including the new Santo Anastácio form. The specimen is still unnamed due to the absence of skull characters that preclude its accurate positioning within the Bauru Group skull-based taxa. In addition, the phylogenetic affinities of this taxon were analyzed into Podocnemidinura clade.

  14. A Lagrangian Physical-Biological Model to Study Water Parcels Associated with Algal Blooms from Southern California Bight to Todos Santos Bay.

    NASA Astrophysics Data System (ADS)

    Vivas Téllez, I. E.; Rivas, D.

    2015-12-01

    Lagrangian ocean circulation and biological dynamics are numerically studied in Todos Santos Bay during the spring of 2007. This period is particularly interesting after an intense toxic algal bloom occurred in April 2007 in this area, which was associated with the wind-driven upwelling in the region. High resolution, numerical model simulations were carried out to study dynamical features along of the Southern California Bight (SCB), the coast of the northern Baja California (BC), and the interior of Todos Santos Bay (TSB). These simulations are used in a three-dimensional Lagrangian (particle tracking) analysis which provides information about the origin and distribution of the waters present in the Bay during the occurrence of the toxic bloom. After the selection of trajectories of particles showing coherent patterns, a Nitrate-Phytoplankton-Zooplankton-Detritus (NPZD) lower trophic model is implemented to study the influence of the environmental conditions that occur during the particle advection, solving the NPZD equations at every time-varying position of the advected particles. The model is also modified for phytoplankton growth as a function of the environmental temperature to somehow emulate the life cycle of Pseudo-nitzschia. The analysis of the trajectories shows that particles mainly come from two regions: from the north, in the southern portion of SCB and from regions west of the TSB. Knowing the regional circulation patterns and their phytoplankton dynamics can help to understand and even predict the origin and destination of the harmful algal blooms that occur in TSB and its surroundings.

  15. Spatial distribution and concentration assessment of total petroleum hydrocarbons in the intertidal zone surface sediment of Todos os Santos Bay, Brazil.

    PubMed

    Silva, Carine S; Moreira, Icaro T A; de Oliveira, Olivia M C; Queiroz, Antonio F S; Garcia, Karina S; Falcão, Brunno A; Escobar, Narayana F C; Rios, Mariana Cruz

    2014-02-01

    The primary objective of this study was to investigate the concentrations and spatial distribution of the total petroleum hydrocarbons (TPHs) in the intertidal zone surface sediment of Todos os Santos Bay, Brazil, to assess the distribution and degree of contamination by TPHs, measure the level of TPH degradation in the surface sediment, and identify the organic matter sources. The surface sediment used in this study was collected in 50 stations, and TPHs, isoprenoid alkanes (pristane and phytane), and unresolved complex mixture (UCM) were analyzed by gas chromatography with a flame ionization detector. The total concentrations ranged from 0.22 to 40,101 μg g(-1) dry weight and showed a strong correlation with the total organic carbon (TOC) content. The highest TPH concentrations were observed in samples from the mangrove sediments of a river located near a petroleum refinery. Compared with other studies in the world, the TPH concentrations in the intertidal surface sediment of Todos os Santos Bay were below average in certain stations and above average in others. An analysis of the magnitude of UCM (0.11 to 17,323 μg g(-1) dry weight) and the ratios nC17/Pr and nC18/Ph suggest that an advanced state of oil weathering, which indicates previous contamination. The molar C/N ratios varied between 5 and 43, which indicate organic matter with a mixed origin comprising marine and continental contributions. PMID:24100798

  16. 10Be dating of river terraces of Santo Domingo river, on Southeastern flank of the Mérida Andes, Venezuela: Tectonic and climatic implications

    NASA Astrophysics Data System (ADS)

    Guzmán, Oswaldo; Vassallo, Riccardo; Audemard, Franck; Mugnier, Jean-Louis; Oropeza, Javier; Yepez, Santiago; Carcaillet, Julien; Alvarado, Miguel; Carrillo, Eduardo

    2013-12-01

    In this study, we discuss the first cosmogenic 10Be dating of river terraces located in the lower reaches of the Santo Domingo river (Southeastern flank of the Mérida Andes, Western Venezuela). The geomorphic observations and dating allowed the restoration of the temporal evolution of incision rate, which was analysed in terms of tectonic, climatic and geomorphic processes. The long-term incision rate in the area has been constantly around 1.1 mm/a over the last 70 ka. Taking into account the geologic and geomorphologic setting, this value can be converted into the Late Pleistocene uplift rate of the Southeastern flank of the Mérida Andes. Our results show that the process of terraces formation in the lower reaches of the Santo Domingo river occurred at a higher frequency (103-104 years) than a glacial/interglacial cycle (104-105 years). According to the global and local climate curve, these terraces were abandoned during warm to cold transitions.

  17. Cadmium and phosphate variability during algal blooms of the dinoflagellate Lingulodinium polyedrum in Todos Santos Bay, Baja California, Mexico.

    PubMed

    Gutierrez-Mejia, E; Lares, M L; Huerta-Diaz, M A; Delgadillo-Hinojosa, F

    2016-01-15

    Dinoflagellate algal blooms (DABs), with Lingulodinium polyedrum as the dominant species, have increased over the past few years in coastal areas off Baja California, Mexico. Vertical and temporal variability of particulate cadmium (Cdp), dissolved Cd (Cdd), PO4(3-) and Cdd/PO4(3-) were investigated during two intense DABs of L. polyedrum that occurred during the fall of 2011 and 2012 in Todos Santos Bay. Results were then, compared with data gathered in the absence of algal blooms during the autumn of 2013. In both algal blooms, L. polyedrum tended to be concentrated near the surface throughout the duration; however, during DAB 2011 the number of cells was twice as abundant ([10.0 ± 8.0] × 10(5) cells L(-1)) as in DAB 2012 ([5.0 ± 4.4] × 10(5) cells L(-1)). During DAB 2011, Cdp increased significantly (up to 1.02 ± 0.99 nmol kg(-1)) and was positively correlated with the cell abundance of L. polyedrum, suggesting that this dinoflagellate is able to assimilate and concentrate Cdd. Likewise, Cdd (up to 0.71 ± 0.17 nM) increased in the days of highest cell abundance, which could be attributed to uptake and subsequent regeneration of Cdd resulting from the remineralization of organic particulate matter produced during the bloom, as well as with the presence of organic ligands secreted by L. polyedrum that could keep Cdd in solution. During DAB 2011, dissolved Cdd/PO4(3-) ratios exhibited high vertical and temporal variability in the upper 5 m of the water column, but remained virtually constant near the bottom, suggesting a depth-dependent decoupling between these two dissolved components during the bloom development. Given the observed differences in the vertical and temporal variability of Cdd, Cdp, and PO4(3-) between these two intense DABs, we propose the existence of an abundance threshold of approximately 10(6) cells L(-1) of L. polyedrum above which Cd and PO4(3-) significantly increased due to remineralization in coastal waters during the bloom

  18. Comparing progress toward the millennium development goal for under-five mortality in León and Cuatro Santos, Nicaragua, 1990–2008

    PubMed Central

    2014-01-01

    Background Social inequality in child survival hampers the achievement of Millennium Development Goal 4 (MDG4). Monitoring under-five mortality in different social strata may contribute to public health policies that strive to reduce social inequalities. This population-based study examines the trends, causes, and social inequality of mortality before the age of five years in rural and urban areas in Nicaragua. Methods The study was conducted in one rural (Cuatro Santos) and one urban/rural area (León) based on data from Health and Demographic Surveillance Systems. We analyzed live births from 1990 to 2005 in the urban/rural area and from 1990 to 2008 in the rural area. The annual average rate reduction (AARR) and social under-five mortality inequality were calculated using the education level of the mother as a proxy for socio-economic position. Causes of child death were based on systematic interviews (verbal autopsy). Results Under-five mortality in all areas is declining at a rate sufficient to achieve MDG4 by 2015. Urban León showed greater reduction (AARR = 8.5%) in mortality and inequality than rural León (AARR = 4.5%) or Cuatro Santos (AARR = 5.4%). Social inequality in mortality had increased in rural León and no improvement in survival was observed among mothers who had not completed primary school. However, the poor and remote rural area Cuatro Santos was on track to reach MDG4 with equitable child survival. Most of the deaths in both areas were due to neonatal conditions and infectious diseases. Conclusions All rural and urban areas in Nicaragua included in this study were on track to reach MDG4, but social stratification in child survival showed different patterns; unfavorable patterns with increasing inequity in the peri-urban rural zone and a more equitable development in the urban as well as the poor and remote rural area. An equitable progress in child survival may also be accelerated in very poor settings. PMID:24428933

  19. Spatial and vertical distribution of metals in sediment cores from Río Espíritu Santo estuary, Puerto Rico, United States.

    PubMed

    Williams, Nekesha; Block, Karin A

    2015-11-15

    The concentration and distribution of Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn were investigated in three sediment cores representing 100-150years of accumulation in upriver, midriver, and estuarine environments in Río Espíritu Santo (RES), Puerto Rico. Grain-size distribution, organic matter and carbonate content were determined to assess their influence on metal concentrations. Minimum biotoxicity levels of Ni and Cu were exceeded in the upriver and midriver sites, while the minimum biotoxicity level of Cu was exceeded in the estuarine location. Pb concentration decreased by ~35% in the upper portion of the midriver and estuarine cores compared to downcore concentrations as a consequence of leaded gasoline regulation. Enrichment Factors and Geoaccumulation Indices were calculated for each metal revealing high levels of Cu in all three cores, likely from an igneous source. Our results provide a baseline for metal contamination in an area facing further land use change. PMID:26337229

  20. Local Site Characterization Using HVSR, ReMi, and SPAC, Study Case: Soccer Field At Autonomous University of Santo Domingo, Dominican Republic

    NASA Astrophysics Data System (ADS)

    Upegui Botero, F. M.; Rojas Mercedes, N.; Huerta-Lopez, C.; Martinez-Cruzado, J. A.; Suárez, L.; Lopez, A. M.; Huerfano Moreno, V.

    2013-12-01

    Earthquake effects are frequently quantified by the energy liberated at the source, and the degree of damage produced in urban areas. The damage of historic events such as the Mw=8.3, September 19, 1985 Mexico City Earthquake was dominated by the amplification of seismic waves due to local site conditions. The assessment of local site effects can be carried out with site response analyses in order to determine the properties of the subsoil such as the dominant period, and the Vs30. The evaluation of the aforementioned properties is through the analysis of ground motion. However, in locations with low seismicity, the most convenient method to assess the site effect is the analysis of ambient vibration measurements. The Spatial Auto Correlation method (SPAC) can be used to determine a Vs30 model from ambient vibration measurements using a triangular array of sensors. Refraction Microtremor (ReMi) considers the phase velocity of the Rayleigh waves can be separated of apparent velocities; the aim of the ReMI method is to obtain the Vs30 model. The HVSR technique or Nakamura's method has been adopted to obtain the resonant frequency of the site from the calculation of ratio between the Fourier amplitude spectra or PSD spectrum of the horizontal and vertical components of ambient vibration. The aim of this work is to compare the results using different techniques to assess local site conditions in the urban area of Santo Domingo, Dominican Republic. The data used was collected during the Pan-American Advance Studies Institute (PASI), Workshop held in Santo Domingo, Dominican Republic from July 14 to 25, 2013. The PASI was sponsored by IRIS Consortium, NSF and DOE. Results obtained using SPAC, and ReMi, show a comparable model of surface waves velocities. In addition to the above, the HVSR method is combined with the stiffness matrices method for layered soils to calculate a model of velocities and the predominant period on the site. As part of this work a comparison with

  1. Review of Dissomphalus Ashmead, 1893 (Hymenoptera, Bethylidae) from Espírito Santo, Brazil, with description of twenty-one new species.

    PubMed

    Colombo, Wesley D; Azevedo, Celso O

    2016-01-01

    Forty-two species of Dissomphalus Ashmead, 1893 are reviewed, 21 of them are described and illustrated: Dissomphalus botocudus sp. nov., D. fredi sp. nov., D. tupinikim sp. nov., D. guarani sp. nov., D. congo sp. nov., D. rosangelae sp. nov., D. w-aedeagus sp. nov., D. amana sp. nov., D. potyra sp. nov., D. pyata sp. nov., D. clovisi sp. nov., D. kuara sp. nov., D. miriamae sp. nov., D. cacirus sp. nov., D. mirim sp. nov., D. secretus sp. nov., D. caparao sp. nov., D. capixaba sp. nov., D. ibirapitanga sp. nov., D. purius sp. nov. and D. taiabocu sp. nov. Six species are registered for the first time from Espírito Santo: D. brasiliensis Kieffer, D. bahiensis Redighieri & Azevedo, D. dumosus Evans, D. incomptus Evans, D. refertus Alencar & Azevedo and D. umbilicus Azevedo. Three species were reidentified: D. napo Evans, D. truncatus Azevedo and D. vallensis Azevedo. Among fifteen species-groups which recognized in the genus, amana species-group; amplus species-group and secretus species-group are newly proposed. PMID:27470834

  2. Molecular epidemiology of HFE gene polymorphic variants (C282Y, H63D and S65C) in the population of Espírito Santo, Brazil.

    PubMed

    Alves, L N R; Santos, E V W; Stur, E; Silva Conforti, A M A; Louro, I D

    2016-01-01

    Hereditary hemochromatosis (HH) is an autosomal recessive disorder that leads to progressive iron accumulation and may cause cirrhosis, hepatocellular carcinoma, diabetes, and heart failure. Most cases of HH have been linked to mutations in genes associated with iron homeostasis. There have been three major variants in the high Fe (HFE) gene associated with the disease: C282Y, H63D and S65C. In this context, we aimed to evaluate the prevalence of the polymorphic variants (C282Y, H63D and S65C) of the HFE gene in the population of the Espírito Santo State (ES), Brazil by analyzing three different groups: general population (N = 120), Pomeranian descendants (N = 59), and patients with HH (N = 20). Using genomic DNA extracted from peripheral blood, polymorphic variant identification was performed by polymerase chain reaction-restriction fragment length polymorphism. Statistically significant differences were observed for genotype distribution of C282Y (P < 0.001) and H63D (P = 0.013) between the general population and the patients diagnosed with HH. This is the first study to analyze HFE gene allele frequencies for the general population, Pomeranian subpopulation, and patients with HH of ES, Brazil. PMID:27173269

  3. [Effects of the installation of the Rosal hydroelectric power station, Itabapoana River, States of Espírito Santo and Rio de Janeiro, on anophelinae, planorbidae and phlebotominae].

    PubMed

    Rezende, Helder Ricas; Sessa, Paulo Augusto; Ferreira, Adelson Luiz; dos Santos, Claudiney Biral; Leite, Gustavo Rocha; Falqueto, Aloísio

    2009-01-01

    The construction of dams usually changes the composition of the fauna within their areas of influence. In the area where the Rosal hydroelectric power station has been installed, in the States of Espírito Santo and Rio de Janeiro, changes to the abundance of anophelinae, planorbidae and phlebotominae were evaluated before (1998-2000) and after (2000-2005) the damming. Nine sample collection points were defined, each represented by a home and its outbuildings, shelters for domestic animals and water collection points within a radius of 150m. One hundred and three adult anophelinae were collected before the damming and 313 afterwards; 200 immature anophelinae before and 708 afterwards; 868 planorbidae before and 486 afterwards; and 2,979 phlebotominae before and 912 afterwards. The vector record, consisting of anophelinae, planorbidae and phlebotominae, shows the potential of this area for transmission of malaria, schistosomiasis and American cutaneous leishmaniasis. However, the environmental changes did not increase the risk of the occurrence of these diseases. PMID:19448934

  4. Survey of Trypanosoma and Leishmania in wild and domestic animals in an Atlantic rainforest fragment and surroundings in the state of Espírito Santo, Brazil.

    PubMed

    Acosta, Igor da C L; Da Costa, Andrea P; Gennari, Solange M; Marcili, Arlei

    2014-05-01

    Trypanosoma and Leishmania infections affect wild and domestic animals and human populations. The growing process of deforestation and urbanization of Atlantic Rainforest areas has given rise to introduction of humans and domestic animals to the sylvatic cycles of Trypanosoma and Leishmania species. Serological, parasitological, and molecular surveys among wild and domestic animals in the Corrego do Veado Biological Reserve, which is an Atlantic Rainforest fragment in the state of Espírito Santo, southeastern Brazil, were evaluated. In total, 154 wild animals of 25 species and 67 domestic animals (47 dogs and 20 horses) were sampled. All the domestic animals were serologically negative for anti-Leishmania infantum chagasi antibodies and negative in parasitological approaches. Only the Order Chiroptera presented positive blood cultures and cryopreserved isolates. The phylogenetic trees based on SSU rDNA and gGAPDH genes confirmed the occurrence of Trypanosoma dionisii and provided the first record of Trypanosoma cruzi marinkellei in southeastern Brazil. The studies conducted in Atlantic Rainforest remaining trees provide the knowledge of parasite diversity or detect parasites that can accelerate the loss of hosts diversity. PMID:24897863

  5. Ossification Pattern of Estuarine Dolphin (Sotalia guianensis) Forelimbs, from the Coast of the State of Espírito Santo, Brazil

    PubMed Central

    Botta, Silvina; de Queiroz, Fábio Ferreira; Campos, Adélia Sepúlveda

    2015-01-01

    The estuarine dolphin, Sotalia guianensis, is one of the most abundant cetacean species in Brazil. Determination of age and of aspects associated with the development of this species is significant new studies. Counts of growth layer groups in dentin are used to estimate age of these animals, though other ways to evaluate development are also adopted, like the measurement of total length (TL). This study presents a procedure to evaluate the development of the estuarine dolphin based on the ossification pattern of forelimbs. Thirty-seven estuarine dolphins found in the state of Espírito Santo, Brazil, were examined. Age was estimated, TL was measured and ossification of epiphyses was examined by radiography. We analyzed results using the Spearman correlation. Inspection of radiographs allowed evaluation of the significance of the correlation between age and development of the proximal (r = 0.9109) and distal (r = 0.9092) radial epiphyses, and of the distal ulnar epiphyses (r = 0.9055). Radiographic analysis of forelimbs proved to be an appropriate method to evaluate physical maturity, and may be a helpful tool to estimate age of these animals in ecological and population studies. PMID:26017269

  6. Chilled margin fragmentation as a trigger for transition from Strombolian to phreatomagmatic explosive activity at Cova de Paul Crater, Santo Antao, Cape Verde Islands

    NASA Astrophysics Data System (ADS)

    Tarff, R. W.; Day, S. J.

    2013-07-01

    Sudden transitions from effusive or mildly explosive eruptions to violent phreatomagmatic explosive activity can occur at high-elevation vents on oceanic island volcanoes. Here, we describe near-vent deposits around the large Cova de Paul crater on Santo Antao, Cape Verde Islands, which provide insights into the transition from Strombolian magmatic to violently explosive phreatomagmatic activity during one such eruption. The Strombolian deposits change from welded spatter to bedded clinkery scoria to angular blocky scoria, and contain a distinct population of strongly banded, low-vesicularity angular sub-glassy clasts with strongly prolate vesicles. These sub-glassy clasts also form a discrete layer of larger blocks at the transition to the overlying phreatomagmatic deposits. They are compositionally identical to the Strombolian scoria and are interpreted to be fragments of flow-banded chilled margins from the walls of the eruptive conduit. The layer of large sub-glassy blocks records disintegration and expulsion of the chilled margins at the transition to phreatomagmatic activity. We interpret the sequence from spatter through clinkery scoria to blocky scoria as recording an increase in groundwater inflow into the conduit from pressurised aquifers, but at coolant input ratios below those required for explosions. The shattering of the chilled margins to produce the bed of large sub-glassy blocks appears to have allowed a much greater water flow into the conduit, leading to the phreatomagmatic explosive phase of the eruption.

  7. Evolutionary escape from the climate dilemma. Comment on "Climate change governance, cooperation and self-organization" by Pacheco, Vasconcelos and Santos

    NASA Astrophysics Data System (ADS)

    Tavoni, Alessandro

    2014-12-01

    Jorge M. Pacheco, Vítor V. Vasconcelos and Francisco C. Santos take stock of a growing body of research they have produced in recent years, with the aim of shedding light on whether bottom-up agreements can suffice in promoting the high levels of cooperation needed to avert dangerous climate change [1]. The current state of affairs leave little reason for optimism, given the steady rise in CO2 emissions [2]. The UNFCCC approach to seeking universal participation has thus been called into question, both by some policy makers and by academics who have established pessimistic theoretical predictions concerning the limited ability of international environmental agreements to improve upon what nation states would do in the absence of an agreement. Game theorists have predicted that self-enforcing agreements are likely to comprise only a handful of countries committing to unambitious emission abatement targets [3]. Clearly, this is incompatible with stabilizing CO2 concentrations at levels that significantly constrain the likelihood of dangerous climate change. The question than arises as to whether we can deliver on ambitious abatement targets by leveraging on unilateral action by countries or other sub- or supra-national entities (e.g. cities or blocs). This has recently been analyzed in game-theoretic contexts [4,5], as well as in the governance literature [6], with more optimistic predictions compared to traditional models focusing on overarching agreements.

  8. Ossification Pattern of Estuarine Dolphin (Sotalia guianensis) Forelimbs, from the Coast of the State of Espírito Santo, Brazil.

    PubMed

    de Carvalho, Anna Paula Martins; Lima, Juliana Ywasaki; Azevedo, Carolina Torres; Botta, Silvina; de Queiroz, Fábio Ferreira; Campos, Adélia Sepúlveda; Barbosa, Lupércio de Araújo; da Silveira, Leonardo Serafim

    2015-01-01

    The estuarine dolphin, Sotalia guianensis, is one of the most abundant cetacean species in Brazil. Determination of age and of aspects associated with the development of this species is significant new studies. Counts of growth layer groups in dentin are used to estimate age of these animals, though other ways to evaluate development are also adopted, like the measurement of total length (TL). This study presents a procedure to evaluate the development of the estuarine dolphin based on the ossification pattern of forelimbs. Thirty-seven estuarine dolphins found in the state of Espírito Santo, Brazil, were examined. Age was estimated, TL was measured and ossification of epiphyses was examined by radiography. We analyzed results using the Spearman correlation. Inspection of radiographs allowed evaluation of the significance of the correlation between age and development of the proximal (r = 0.9109) and distal (r = 0.9092) radial epiphyses, and of the distal ulnar epiphyses (r = 0.9055). Radiographic analysis of forelimbs proved to be an appropriate method to evaluate physical maturity, and may be a helpful tool to estimate age of these animals in ecological and population studies. PMID:26017269

  9. Perspectivas Pedagogicas. Documentos-Ponencias Y Ensayos Proyecto Teacher Corps, Ciclo XII (Pedagogical Perspectives. Documents, Papers and Essays, Teacher Corps Project, Cycle XII).

    ERIC Educational Resources Information Center

    Albino, Isidra, Ed.; Davila, Sonia, Ed.

    The purpose of this book is to stimulate ideas leading to a sharing of approaches, strategies, and methodologies applicable to the education of Puerto Ricans. Following introductory material, 18 papers are presented, the first 10 of which are in Spanish. Titles and authors are: (1) "El maestro que Puerto Rico necesita," Leonides Santos Vargas; (2)…

  10. Dolines in Sierra de Quemado and their relationship with the development of the Gran Caverna de Santo Tomàs, Vinales, Cuba

    NASA Astrophysics Data System (ADS)

    Farfan Gonzalez, Hermes; Diaz Guanche, Carlos; Aldana Vilas, Carlos

    2010-05-01

    Dolines are the most representative landforms of karst territories, and have a very significant development in the tropical karst. In the Sierra de los Órganos (West Cuba), the doline development in size and depth has been taken in consideration in several papers, especially after the classic works of Herbert Lehman in the 50's. Recent studies have revealed that horizontal and vertical development of doline is conditioned by tectonics, their spatial location, and is strongly controlled by the local base level. In the Sierra de Quemado, about 30 dolines were recognized with a remarkable variability in shape, size and depth. They are mainly collapsed dolines with different deepening phases, clearly marking the various evolutionary stages of the system, and working as individual entities to control the surface erosion, notably influencing the configuration of the main karst system such as the Gran Caverna de Santo Tomás. Collapse dolines originate as a consequence of the loss of mass, due to speleogenetic processes and the likely fall of the roofs of caves that developed below the dolines. The successive evolution of dolines often took place for the proximity of a cave (active or not), evolving parallelly. When doline deepening reached this level, the retraction of the walls began, which also had an influence on the hypodermic cavities observed in the dolines, and on some foot-caves ("marginal caves" or "füsshölen") that acted as important recharge points. Instability of part of the slope will cause its final collapse. Vertical jointing and faulting, together with the high values in the angles of internal friction, thoroughly contribute to the process of slope recession. Because of their different phases and evolutionary stages, the ranges in the morphometric values of dolines are high: some dolines have dimensions similar to "Tiankeng", with more than 100 metres in diameter and depth. As a rule, the different deepening phases that mark the evolutionary stages of

  11. Probing The Buried Remains of The Todos los Santos, City of San Salvador in Hoping Island with Shallow Subsurface Geophysics Method

    NASA Astrophysics Data System (ADS)

    Tsai, Yi-fan; Chang, Pin-yu; Eugenio Borao Mateo, José

    2013-04-01

    The study in ancient sites with GPR is widely documented over several decades. This non-invasive geophysical method provides a rapid measure for anthropogenic objects and therefore serves as a guide for possible excavation for the next stage of archaeological surveys. City of San Salvador, which is a Dutch colonial city consisted of fortress, hospitals and churches in 17 century, is located in the Hoping Island in Keelung, Taiwan. The fortress and its affiliated structures were abandoned and left collapsing since the mid-17th century. Some relics of the fortress wall were still remained until the early 20th century but the fast development projects in the island has caused the relics demolished or buried under building or road pavements. Many wells and bones have been found around the area belong to over three hundred years ago. As a consequence, the government initiated a new excavation project at the parking lot where the ancient convent of Todos los Santos is believed since 2011 in order to find the remains of the convent in city of San Salvador. Meanwhile we have surveyed with GPR to help guiding the excavation location. In this case, we surveyed with wide-angle-refraction/reflection (WARR) of GPR as well as common-offset array, to compensate the defect of traditional common-offset of lack of longitudinal resolution with velocity profile, and the combination of velocity profile and common-offset data helped distinguish the signals from other noises and further located the position of subsurface structures. After data analysis and numerical modeling of the buried materials, we have located the possible remains of walls of the convent or other structures around 0.9 to 1.3 meters in depth that can offer useful information to better plan the archaeological excavations.

  12. CUTANEOUS TUBERCULOSIS: A 26-YEAR RETROSPECTIVE STUDY IN AN ENDEMIC AREA OF TUBERCULOSIS, VITÓRIA, ESPÍRITO SANTO, BRAZIL

    PubMed Central

    SPELTA, Karla; DINIZ, Lucia M.

    2016-01-01

    SUMMARY Background: Tuberculosis is a serious health problem in Brazil so that the knowledge on the aspects of cutaneous tuberculosis is medically important. Objective: To assess the characteristics of patients with cutaneous tuberculosis treated at the Cassiano Antonio Moraes University Hospital, located in the city of Vitória, State of Espírito Santo, Brazil. Methods: This is a retrospective, descriptive, observational and cross-sectional study using the medical records of 29 patients with cutaneous tuberculosis treated at the Dermatology and Pulmonology services of the hospital from 1986 to 2011. The inclusion criterion was the confirmation of cutaneous tuberculosis taking into account clinical, epidemiological, immunological, and bacteriological findings, as well as the response to specific treatment. Results: Of the 29 studied patients; 18 (62%) were women with average age of 37 years; the predominant clinical condition was erythema induratum of Bazin in 12 (41.4%) cases; and the cutaneous lesions were in the lower limbs in 19 (65.8%) patients. Extra-cutaneous involvement occurred in eight (27.6%) cases. The tuberculin tests were positive in 15 (79%) individuals and the assessment of the infectious agent was negative in most of the investigated cases. Conclusion: The study found a low frequency (0.44%) of cutaneous tuberculosis in an endemic area of tuberculosis. There was a predominance of infection in women aged thirty to forty years. Erythema induratum was the most common clinical condition, affecting mainly the lower limbs, in contrast to other Brazilian studies that found scrofuloderma as the most common manifestation, predominating in the cervical region of male children and adolescents. PMID:27410909

  13. Transmitted Drug Resistance Among Antiretroviral-Naive Patients with Established HIV Type 1 Infection in Santo Domingo, Dominican Republic and Review of the Latin American and Caribbean Literature

    PubMed Central

    Taylor, Barbara S.; Rojas Fermín, Rita A.; Reyes, Emily Virginia; Vaughan, Catherine; José, Lina; Javier, Carmen; Franco Estévez, Ramona; Donastorg Cabral, Yeycy; Batista, Arelis; Lie, Yolanda; Coakley, Eoin; Hammer, Scott M.; Brudney, Karen

    2012-01-01

    Abstract Emergence of HIV resistance is a concerning consequence of global scale-up of antiretroviral therapy (ART). To date, there is no published information about HIV resistance from the Dominican Republic. The study's aim was to determine the prevalence of transmitted drug resistance (TDR) to reverse transcriptase and protease inhibitors in a sample of chronically HIV-1-infected patients in one clinic in Santo Domingo. The data are presented in the context of a review of the TDR literature from Latin America and the Caribbean. Genotype testing was successfully performed on 103 treatment-naive adults planning to initiate antiretroviral therapy; the World Health Organization (WHO) list of surveillance drug resistance mutations (SDRM) was used to determine the presence of TDR mutations. WHO SDRM were identified in eight patients (7.8%); none had received sdNVP. There were no significant differences in epidemiologic or clinical variables between those with or without WHO SDRM. The prevalence of WHO SDRM was 1.0% and 6.8% for nucleoside reverse transcriptase inhibitors and nonnucleoside reverse transcriptase inhibitors, respectively. No WHO SDRMs for protease inhibitors were identified. Among 12 studies of TDR in the region with a sample size of at least 100 subjects, the reported prevalence of SDRM ranged from 2.8% to 8.1%. The most commonly identified SDRM was K103N. This information adds to our understanding of the epidemiology of TDR in the region and the possible role such mutations could play in undermining first-line treatment. Ongoing surveillance is clearly needed to better understand the TDR phenomenon in the Caribbean. PMID:21851324

  14. Evaluation of optimal conditions for determination of low selenium content in shellfish samples collected at Todos os Santos Bay, Bahia, Brazil using HG-AFS.

    PubMed

    Lopes Dos Santos, Walter Nei; Macedo, Samuel Marques; Teixeira da Rocha, Sofia Negreiros; Souza de Jesus, Caio Niela; Cavalcante, Dannuza Dias; Hatje, Vanessa

    2014-08-01

    This work proposes a procedure for the determination of total selenium content in shellfish after digestion of samples in block using cold finger system and detection using atomic fluorescent spectrometry coupled hydride generation (HG AFS). The optimal conditions for HG such as effect and volume of prereduction KBr 10 % (m/v) (1.0 and 2.0 ml) and concentration of hydrochloric acid (3.0 and 6.0 mol L(-1)) were evaluated. The best results were obtained using 3 mL of HCl (6 mol L(-1)) and 1 mL of KBr 10 % (m/v), followed by 30 min of prereduction for the volume of 1 mL of the digested sample. The precision and accuracy were assessed by the analysis of the Certified Reference Material NIST 1566b. Under the optimized conditions, the detection and quantification limits were 6.06 and 21.21 μg kg(-1), respectively. The developed method was applied to samples of shellfish (oysters, clams, and mussels) collected at Todos os Santos Bay, Bahia, Brazil. Selenium concentrations ranged from 0.23 ± 0.02 to 3.70 ± 0.27 mg kg(-1) for Mytella guyanensis and Anomalocardia brasiliana, respectively. The developed method proved to be accurate, precise, cheap, fast, and could be used for monitoring Se in shellfish samples. PMID:24771464

  15. A survey of adulterants used to cut cocaine in samples seized in the Espírito Santo State by GC-MS allied to chemometric tools.

    PubMed

    de Souza, Lindamara M; Rodrigues, Rayza R T; Santos, Heloá; Costa, Helber B; Merlo, Bianca B; Filgueiras, Paulo R; Poppi, Ronei J; Vaz, Boniek G; Romão, Wanderson

    2016-03-01

    Cocaine is a stimulant drug of the central nervous system (CNS) extracted from the leaves of Erytroxylum coca. It is defined as a tropane alkaloid containing 1R-(exo,exo)-3-(benzoyloxy)-8-methyl-8-azabicyclo[3.2.1]octane-2-carboxylic acid methyl esther. However, despite its defined composition, a wide variety of chemical additives are present in cocaine found in the illicit market, such as benzocaine, lidocaine, caffeine, procaine and phenacetin. In this work, 512 cocaine samples seized by the Civil Police of Espirito Santo state (PC-ES, Brazil) were analyzed by gas chromatography mass spectrometry (GC-MS) allied to principal component analysis (PCA) in order to classify the samples as a function of seizure year (2008, 2009, 2010, 2011 and 2012) and location (metropolitan, north, south and central). The cocaine content (wt.%) and its adulterants were also estimated. Analyzing the samples seized between 2008 and 2011, three sample sets are clearly grouped according to the degree of adulteration with caffeine and lidocaine: 100-50 wt.% of cocaine; 50-20 wt.% of cocaine; and 20-80 wt.% of lidocaine and 60-80 wt.% of caffeine, simultaneously. The last group is formed by samples seized between 2008 and 2009, which proves the higher degree of adulteration during this period. In 2012, higher cocaine content was observed for the 191 analyzed samples than in samples from previous years. The PCA data also suggests that the metropolitan region samples had a higher degree of adulteration than the state countryside samples. PMID:26976463

  16. Bioavailability assessment of toxic metals using the technique "acid-volatile sulfide (AVS)-simultaneously extracted metals (SEM)" in marine sediments collected in Todos os Santos Bay, Brazil.

    PubMed

    Silva, Jucelino B; Nascimento, Rodrigo A; de Oliva, Sergio T; de Oliveira, Olívia M C; Ferreira, Sergio L C

    2015-10-01

    This paper reports the bioavailability of the metals (cadmium, copper, zinc, lead, and nickel) in sediment samples collected in seven stations from the São Paulo Estuary, Todos os Santos Bay, Brazil. The bioavailability was determined by employing the technique "acid-volatile sulfide (AVS) and simultaneously extracted metal (SEM)". The elements cadmium, copper, lead, and zinc were determined using differential pulse anodic stripping voltammetry (DPASV), while nickel was quantified utilizing electrothermal atomic absorption spectrometry (ET AAS). The accuracy of these methods was confirmed using a certified reference material of estuarine sediment (NIST 1646). The sulfide was quantified using potentiometry with selective electrode and the organic matter determination employing an indirect volumetric method using potassium dichromate and iron(II) sulfate solutions. The bioavailability of the metals was estimated by relationship between the concentration of AVS and the sum of the concentrations of the simultaneously extracted metals (ΣSEM), considering a significant toxicity when (ΣSEM)/(AVS) is higher than 1. The bioavailability values in the seven stations studied varied from 0.93 to 1.31 (June, 2014) and from 0.34 to 0.58 (September, 2014). These results demonstrated a critical condition of toxicity (bioavailability >1) in six of the seven sediment samples collected during the rainy season (June, 2014). In the other period (September, 2014), the bioavailability was always lower than 1 for all sediment samples collected in the seven stations. The individual values of the concentrations of the five metals were compared with the parameters PEL (probable effects level) and TEL (threshold effects level), which are commonly employed for characterization of ecological risk in environmental systems. This comparison revealed that all metals have concentrations lower than the PEL and only zinc and lead in some stations have contents higher than the TEL. The

  17. Petrography, geochemistry, and depositional setting of the San Pedro and Santo Tomas coal zones: anomalous algae-rich coals in the middle part of the Claiborne Group (Eocene) of Webb County, Texas

    USGS Publications Warehouse

    Warwick, P.D.; Hook, R.W.

    1995-01-01

    Two coal zones, the San Pedro and the overlying Santo Tomas, are presented for nearly 35 km in outcrop, surface and underground mines, and shallow drill holes along the strike of the middle part of the Claiborne Group (Eocene) in Webb County, Texas. A sandstone-dominated interval of 25 to 35 m separates the two coal zones, which range up to 3 m in thickness. The coal-bearing portion of the middle Claiborne Group in the Rio Grande area represents a fining-upward transition from sandstone-dominated, marine-influenced, lower delta plain depositional environments to more inland, mudstone-rich, predominantly freshwater deltaic settings. The less variable nature of the Santo Tomas coal zone reflects its origin in the upper part of an interlobe basin that received only minor clastic influx. Petrographic attributes of the nonbanded coals indicate that they formed subaqueously in fresh to possibly brackish waters. Petrographic study of polished blocks indicates that approximately 10% of the nonbanded coal from both coal zones is composed of green algae fructifications. -from Authors

  18. Assessment of Mass-Transport Deposits occurrence offshore Espírito Santo Basin (SE Brazil) using a bivariate statistical method

    NASA Astrophysics Data System (ADS)

    Piedade, Aldina; Alves, Tiago; Luís Zêzere, José

    2016-04-01

    Mass Transport Deposits (MTDs) are one of the most important process shaping passive and active margins. It is frequently happening and its characteristics, features and processes has been very well documented from diverse approaches and methodologies. In this work a methodology for evaluation of MTDs occurrence is tested in an area offshore Espírito Santo Basin, SE Brazil. MTDs inventory was made on three-dimensional (3D) seismic volume interpreting a high amplitude reflection which correspond to the top and base of the MTDs. The inventory consists of four MTDs which were integrated into a GIS database. MTDs favourability scores are computed using algorithms based on statistical/probabilistic analysis (Information Value Method) over unique condition terrain units in a raster basis. Terrain attributes derived from the Digital Terrain Model (DTM) are interpreted as proxies of driving factors of MTDs and are used as predictors in our models which are based on a set of different MTDs inventories. Three models are elaborated independently according to the area of the MTDs body (Model 1, Model 2 and Model 3). The final result is prepared by sorting all pixels according to the pixel favourability value in descending order. The robustness and accuracy of the MTDs favourability models are evaluated by the success-rate curves, which are used for the quantitative interpretation of the models expressing the goodness of fit of the MTDs. In addition, a sensitivity analysis was performed and the predisposing factors which have highest prediction performance on MTDs occurrence were identified. The obtained results allow to conclude the method is valid to apply to submarine slopes as it is demonstrated by the highest obtained goodness of fit (0.862). This work is very pioneer, the methodology used was never applied to submarine environment. It is a very promising and valid methodology within the prediction of submarine slopes regarding failing and instability to the industry. In

  19. GIS applied to agriclimatological zoning and agrotoxin residue monitoring in tomatoes: A case study in Espírito Santo state, Brazil.

    PubMed

    dos Santos, Gleissy Mary Amaral Dino Alves; Dos Santos, Alexandre Rosa; Teixeira, Luciano José Quintão; Saraiva, Sérgio Henriques; Freitas, Deivid França; Pereira, Olavo dos Santos; Ribeiro, Carlos Antonio Alvares Soares; Lorenzon, Alexandre Simões; Eugenio, Fernando Coelho; Neves, Antônio Augusto; de Queiroz, Maria Eliana Lopes Ribeiro; Scherer, Rodrigo

    2016-01-15

    Searches related to global warming have provided important insights into the response of terrestrial ecosystems, but few have examined the impacts on agricultural crops, particularly those associated with the monitoring of agrotoxin residues. In this context, the agriclimatological zoning is an important tool in the planning and consolidation of crops and should be considered in any initiative that involves such planning. This tool is particularly important in the analysis of agrotoxin residues and may be applied by the Program Analysis of Agrotoxin Residues in Food (PARA) created by the National Health Vigilance Agency of Brazil (ANVISA), which enables greater food security and contributes to the improvement of human health. The aim of this study was to elaborate the current and future agriclimatological zoning for the tomato crop, relating it with the monitoring of samples collected by PARA in Espírito Santo State, Brazil. The results indicate that a temperature increase of 5 °C creates a decrease in apt areas from 37.3% to 4.3%, for a total reduction of 33 percentage points (-88.5%). It is noted that of the 41 producing municipalities, only 26 have apt areas greater than 50%, highlighting the municipalities with apt areas greater than 90%, represented by Mantenópolis (100%), Guaçuí (98.5%), São José do Calçado (97.8%), Irupi (94.4%), Santa Teresa (92.3%), and Marechal Floriano (91.4%). The veracity of agriclimatological zoning is proved by a Kendall rank correlation coefficient of 0.876, indicating that the distribution of the variables of apt areas and productivity are similar at the significance level of 0.05 with a confidence interval 95%. After validation of the agriclimatological zoning for the tomato crop, it is recommended that the PARA should monitor 36 municipalities rather than the current 18, representing an increase of 100%. The methodology can be adjusted to agricultural crops of other countries. PMID:26555099

  20. Fruit flies (Diptera, Tephritidae) and their associations with native host plants in a remnant area of the highly endangered Atlantic Rain Forest in the State of Espírito Santo, Brazil.

    PubMed

    Uramoto, K; Martins, D S; Zucchi, R A

    2008-10-01

    The results presented in this paper refer to a host survey, lasting approximately three and a half years (February 2003-July 2006), undertaken in the Vale do Rio Doce Natural Reserve, a remnant area of the highly endangered Atlantic Rain Forest located in Linhares County, State of Espírito Santo, Brazil. A total of 330 fruit samples were collected from native plants, representing 248 species and 51 plant families. Myrtaceae was the most diverse family with 54 sampled species. Twenty-eight plant species, from ten families, are hosts of ten Anastrepha species and of Ceratitis capitata (Wiedemann). Among 33 associations between host plants and fruit flies, 20 constitute new records, including the records of host plants for A. fumipennis Lima and A. nascimentoi Zucchi. The findings were discussed in the light of their implications for rain forest conservation efforts and the study of evolutionary relationships between fruit flies and their hosts. PMID:18439337

  1. Henrique da Rocha Lima.

    PubMed

    Bernardes Filho, Fred; Avelleira, João Carlos Regazzi

    2015-01-01

    Brazilian physician and researcher Henrique da Rocha Lima was born in 1879 in the city of Rio de Janeiro, where he studied medicine and obtained the degree of M.D. in 1901. He specialized in Clinical Medicine in Germany and was the ambassador in European countries of the scientific medicine that emerged from the Oswaldo Cruz Institute in the early twentieth century. Rocha Lima has discovered the causative agent of typhus and had a major contribution to the studies of yellow fever, Chagas disease, Carrión's disease and histoplasmosis. His genius, his research and his discoveries projected his name, and, with it, the image of Brazil in the international scientific scene. PMID:26131867

  2. Henrique da Rocha Lima*

    PubMed Central

    Bernardes Filho, Fred; Avelleira, João Carlos Regazzi

    2015-01-01

    Brazilian physician and researcher Henrique da Rocha Lima was born in 1879 in the city of Rio de Janeiro, where he studied medicine and obtained the degree of M.D. in 1901. He specialized in Clinical Medicine in Germany and was the ambassador in European countries of the scientific medicine that emerged from the Oswaldo Cruz Institute in the early twentieth century. Rocha Lima has discovered the causative agent of typhus and had a major contribution to the studies of yellow fever, Chagas disease, Carrión’s disease and histoplasmosis. His genius, his research and his discoveries projected his name, and, with it, the image of Brazil in the international scientific scene. PMID:26131867

  3. The Moon Phases in a Paper Box. (Spanish Title: Las Fases de la Luna en Una Caja de Cartón.) As Fases da Lua Numa Caixa de Papelão

    NASA Astrophysics Data System (ADS)

    de Fátima O. Saraiva, Maria; Amador, Cláudio B.; Kemper, Érico; Goulart, Paulo; Muller, Angela

    2007-12-01

    material didático de baixo custo para demonstração do conceito de fases de um corpo iluminado. O principal objetivo de nosso material é facilitar a compreensão das fases da Lua da perspectiva de um observador na Terra. O material ajuda na visualização de dois efeitos importantes: (1º) mesmo tendo sempre a metade da "Lua" (representada por uma bolinha de isopor ou de ping-pong) iluminada pelo "Sol" (representado por uma fonte de luz natural ou artificial), nós vemos diferentes frações de sua superfície iluminada, dependendo do ângulo pelo qual a olhamos; (2º) a orientação da borda convexa da Lua nas fases Crescente e Minguante também depende da perspectiva pela qual a olhamos da Terra. O uso de uma caixa fechada permite observar o contraste entre as diferentes fases sem necessidade de estar em uma sala escurecida. Apresentamos também um texto explicativo sobre fases da Lua, enfatizando a dependência da aparência da parte iluminada com o ângulo de visada.

  4. Conduit Wall Failure as a Trigger for Transition From Strombolian to Phreatomagmatic Explosive Activity in the Cova de Paúl Crater Eruption on Santo Antão, Cape Verde Islands

    NASA Astrophysics Data System (ADS)

    Tarff, R. W.; Day, S. J.

    2011-12-01

    Episodes of hazardous phreatomagmatic explosive activity, including Surtseyan activity, occur within otherwise less dangerous effusive to mildly explosive magmatic eruptions at high-elevation vents on many oceanic island volcanoes. The water driving these explosions is sourced from freshwater aquifers within the volcanic edifices. Understanding volcanic and geophysical precursors to, and mechanisms of, the (frequently abrupt) transitions to explosive activity is required as a basis for effective warning and mitigation of the resulting hazards. Here we describe near-vent deposits around the large Cova de Paúl crater on the island of Santo Antão, Cape Verde Islands, which provide some insights into a transition from mild magmatic to violently explosive phreatomagmatic activity in one such eruption. This pre-historic but well-preserved crater formed in a single eruption that produced extensive low-temperature, lithic-rich phreatomagmatic pyroclastic flows and surge deposits; these are interbedded in proximal outcrops with airfall breccia and ash beds containing varying proportions of lithic and juvenile clasts, pointing to a series of climactic explosions within an extended period of milder explosive activity of broadly Surtseyan type. Prior to the transition to phreatomagmatic activity, the eruption had been characterized by mild Strombolian activity that produced scoria and spatter deposits of broadly tephritic composition. The Strombolian deposits contain a distinct population of strongly banded, low-vesicularity angular clasts with strongly prolate vesicles and a notably glassy appearance. These became markedly larger and more abundant just below the transition to the phreatomagmatic deposits. Comparisons of these clasts with the Strombolian scoria suggest that they are fragments of flow-banded chilled margins from the walls of the eruptive conduit. Thermal shattering of these margins to produce the angular glassy clasts may record the onset of groundwater flow

  5. Cost-effectiveness of community health worker versus home-based guardians for directly observed treatment of tuberculosis in Vitória, Espírito Santo State, Brazil

    PubMed Central

    do Prado, Thiago Nascimento; Wada, Nikolas; Guidoni, Leticia Molino; Golub, Jonathan E.; Dietze, Reynaldo; Maciel, Ethel Leonor Noia

    2013-01-01

    The objective of this study was to compare the costs and outcomes associated with guardian-supervised directly observed treatment relative to the standard of care Directly Observed Therapy, Short Course (DOTS) provided by community health workers (CHW). New cases of culture-positive pulmonary tuberculosis (TB) treated in Vitória, Espírito Santo State, Brazil, between January 2005 and December 2006 were interviewed and chose their preferred treatment strategy. Costs incurred by providers and patients (and patients’ families) were estimated, and cost-effectiveness was assessed by comparing costs per successfully treated patient. 130 patients were included in the study; 84 chose CHW-supervised DOTS and 46 chose guardian-supervised DOTS. 45 of 46 (98%) patients treated with guardian-supervised DOTS were cured or completed treatment compared to 70/84 (83%) of the CHW-supervised patients (p = 0.01). Logistic regression showed only the strategy of supervision to be a significant association with treatment outcome, with guardian-supervised care strongly protective. Cost per patient treated with guardian-supervised DOTS was US$398, compared to US$548 for CHW-supervised DOTS. The guardian-supervised DOTS is an attractive option to complement CHW-supervised DOTS. PMID:21655845

  6. Considerations on the effects of tidal regimes in the movement of floating litter in an estuarine environment: Case study of the estuarine system of Santos-São Vicente, Brazil.

    PubMed

    Fernandino, Gerson; Elliff, Carla Isobel; Frutuoso, Gabriela Amado; Silva, Eric Vinícius Nascimento Malaquias da; Gama, Guilherme Santiago; Sousa, João Henrique de Oliveira; Silva, Iracema Reimão

    2016-09-15

    Floating litter in the estuarine system of Santos-São Vicente is common and is part of day-to-day activities of various users of the area. The objective of the present study was to carry out a quali-quantitative evaluation of the occurrence of floating litter, to infer their sources, and to identify environmental factors that are likely to control occurrence and distribution, with particular emphasis on the effects of tidal regimes. Six sampling stations were selected along the aforementioned estuary and visited monthly between July 2010 and January 2012. Floating litter was counted from a fixed sampling station. Plastics prevailed (89.64%) and their main source was domestic activities (55.41%). More litter was found during ebb spring tides, with higher concentrations obeying confluence patterns of the estuary's channels. Results indicated that occurrence can be attributed to the deficiency in basic sewage system in the area and the deliberate disposal into the estuary by the local population. PMID:27263980

  7. Leonardo da Vinci and the Downburst.

    NASA Astrophysics Data System (ADS)

    Gedzelman, Stanley David

    1990-05-01

    Evidence from the drawings, experiments, and writings of Leonardo da Vinci are presented to demonstrate that da Vinci recognized and, possibly, discovered the downburst and understood its associated airflow. Other early references to vortex flows resembling downbursts are mentioned.

  8. The major histocompatibility complex genes impact pain response in DA and DA.1U rats.

    PubMed

    Guo, Yuan; Yao, Fan-Rong; Cao, Dong-Yuan; Li, Li; Wang, Hui-Sheng; Xie, Wen; Zhao, Yan

    2015-08-01

    Our recent studies have shown that the difference in basal pain sensitivity to mechanical and thermal stimulation between Dark-Agouti (DA) rats and a novel congenic DA.1U rats is major histocompatibility complex (MHC) genes dependent. In the present study, we further used DA and DA.1U rats to investigate the role of MHC genes in formalin-induced pain model by behavioral, electrophysiological and immunohistochemical methods. Behavioral results showed biphasic nociceptive behaviors increased significantly following the intraplantar injection of formalin in the hindpaw of DA and DA.1U rats. The main nociceptive behaviors were lifting and licking, especially in DA rats (P<0.001 and P<0.01). The composite pain scores (CPS) in DA rats were significantly higher than those in DA.1U rats in both phases of the formalin test (P<0.01). Electrophysiological results also showed the biphasic increase in discharge rates of C and Aδ fibers of L5 dorsal root in the two strains, and the net change of the discharge rate of DA rats was significantly higher than that of DA.1U rats (P<0.05). The mechanical thresholds decreased after formalin injection in both strains (P<0.01), and the net change in the mechanical threshold in DA was greater than that in DA.1U rats (P<0.05). The expression of RT1-B, representation of MHC class II molecule, in laminae I-II of L4/5 spinal cord in DA rats was significantly higher than that in DA.1U rats in the respective experimental group (P<0.05). These results suggested that both DA and DA.1U rats exhibited nociceptive responses in formalin-induced pain model and DA rats were more sensitive to noxious chemical stimulus than DA.1U rats, indicating that MHC genes might contribute to the difference in pain sensitivity. PMID:25861730

  9. Fluorine substitution enhanced photovoltaic performance of a D-A(1)-D-A(2) copolymer.

    PubMed

    Dang, Dongfeng; Chen, Weichao; Yang, Renqiang; Zhu, Weiguo; Mammo, Wendimagegn; Wang, Ergang

    2013-10-18

    A new alternating donor-acceptor (D-A1-D-A2) copolymer containing two electron-deficient moieties, isoindigo and quinoxaline, was synthesized. The photovoltaic performance of this polymer could be improved by incorporating fluorine atoms into the quinoxaline units, resulting in an efficiency of 6.32%. This result highlights the attractive promise of D-A1-D-A2 copolymers for high-performance bulk heterojunction solar cells. PMID:24000353

  10. Autocorrelation descriptor improvements for QSAR: 2DA_Sign and 3DA_Sign.

    PubMed

    Sliwoski, Gregory; Mendenhall, Jeffrey; Meiler, Jens

    2016-03-01

    Quantitative structure-activity relationship (QSAR) is a branch of computer aided drug discovery that relates chemical structures to biological activity. Two well established and related QSAR descriptors are two- and three-dimensional autocorrelation (2DA and 3DA). These descriptors encode the relative position of atoms or atom properties by calculating the separation between atom pairs in terms of number of bonds (2DA) or Euclidean distance (3DA). The sums of all values computed for a given small molecule are collected in a histogram. Atom properties can be added with a coefficient that is the product of atom properties for each pair. This procedure can lead to information loss when signed atom properties are considered such as partial charge. For example, the product of two positive charges is indistinguishable from the product of two equivalent negative charges. In this paper, we present variations of 2DA and 3DA called 2DA_Sign and 3DA_Sign that avoid information loss by splitting unique sign pairs into individual histograms. We evaluate these variations with models trained on nine datasets spanning a range of drug target classes. Both 2DA_Sign and 3DA_Sign significantly increase model performance across all datasets when compared with traditional 2DA and 3DA. Lastly, we find that limiting 3DA_Sign to maximum atom pair distances of 6 Å instead of 12 Å further increases model performance, suggesting that conformational flexibility may hinder performance with longer 3DA descriptors. Consistent with this finding, limiting the number of bonds in 2DA_Sign from 11 to 5 fails to improve performance. PMID:26721261

  11. Virtual observatory publishing with DaCHS

    NASA Astrophysics Data System (ADS)

    Demleitner, M.; Neves, M. C.; Rothmaier, F.; Wambsganss, J.

    2014-11-01

    The Data Center Helper Suite DaCHS is an integrated publication package for building VO and Web services, supporting the entire workflow from ingestion to data mapping to service definition. It implements all major data discovery, data access, and registry protocols defined by the VO. DaCHS in this sense works as glue between data produced by the data providers and the standard protocols and formats defined by the VO. This paper discusses central elements of the design of the package and gives two case studies of how VO protocols are implemented using DaCHS' concepts.

  12. Origin of the DA and non-DA white dwarf stars

    NASA Technical Reports Server (NTRS)

    Shipman, Harry L.

    1989-01-01

    Various proposals for the bifurcation of the white dwarf cooling sequence are reviewed. 'Primordial' theories, in which the basic bifurcation of the white dwarf sequence is rooted in events predating the white dwarf stage of stellar evolution, are discussed, along with the competing 'mixing' theories in which processes occurring during the white dwarf stage are responsible for the existence of DA or non-DA stars. A new proposal is suggested, representing a two-channel scenario. In the DA channel, some process reduces the hydrogen layer mass to the value of less than 10 to the -7th. The non-DA channel is similar to that in the primordial scenario. These considerations suggest that some mechanism operates in both channels to reduce the thickness of the outermost layer of the white dwarf. It is also noted that accretion from the interstellar medium has little to do with whether a particular white dwarf becomes a DA or a non-DA star.

  13. Measurement of D->A Momentum Aperture and Test of D->A Field Qualities

    SciTech Connect

    Halling, Mike

    1992-02-27

    The data presented here were taken during two shifts dedicated to D->A studies. The goal during both of these study periods was a test of the field quality of the D->A channel devices, but for the first study period on 10/21/91 the TBT system was not operational so we simply measured the transfer efficiency as a function of momentum. The conclusion from these measurements is as follows: (1) The momentum aperture of the D->A channel is smaller than expected. (2) The restriction is in physical space is somewhere near A:IKIK. (3) The field quality of the injection channel devices is adequate.

  14. The local effect of octreotide on mechanical pain sensitivity is more sensitive in DA rats than DA.1U rats.

    PubMed

    Yao, Fan-Rong; Wang, Hui-Sheng; Guo, Yuan; Zhao, Yan

    2016-02-01

    A recent study by the authors indicated that major histocompatibility complex (MHC) genes are associated with the differences in basal pain sensitivity and in formalin model between Dark-Agouti (DA) and novel congenic DA.1U rats, which have the same genetic background as DA rats except for the u alleles of MHC. The objective of the present study is to investigate whether there is a difference in the pristane-induced arthritis (PIA) model and local analgesic effect of octreotide (OCT) between DA and DA.1U rats. The hindpaw mechanical withdrawal threshold (MWT) and heat withdrawal latency (HWL) were observed. The C unit firings of the tibial nerve evoked by non-noxious and noxious toe movements were recorded by electrophysiological methods in normal and PIA models in DA and DA.1U rats before and after local OCT administration. The expression of somatostatin receptor 2A (SSTR2A) was observed by immunohistochemistry. The results demonstrate that DA rats have a higher mechanical sensitivity than DA.1U rats after PIA. Local OCT administration significantly elevated MWT in DA rats under normal and PIA sate, but not in DA.1U rats. The electrophysiological experiments showed OCT significantly attenuated the firings of C units evoked by non-noxious and noxious stimulation in DA rats more than those in DA.1U rats both in normal and PIA states. In addition, the expression of SSTR2A in the dorsal horn of the spinal cord was significantly higher in DA than in DA.1U rats. All of the findings suggest a higher local analgesic effect of OCT in DA rats than DA.1U rats, which might be associated with the MHC genes. PMID:26606866

  15. Common colds on Tristan da Cunha

    PubMed Central

    Shibli, M.; Gooch, S.; Lewis, H. E.; Tyrrell, D. A. J.

    1971-01-01

    Eight epidemics of respiratory disease have been observed among islanders of Tristan da Cunha. They seem to be initiated by the arrival of ships and transmission seemed to occur as a result of close human contact but could not always be traced. Islanders suffered from less colds than those in less isolated communities. PMID:5282927

  16. The PAN-DA data acquisition system

    SciTech Connect

    Petravick, D.; Berg, D.; Berman, E.; Bernett, M.; Constanta-Fanourakis, P.; Dorries, T.; Haire, M.; Kaczar, K; MacKinnon, B.; Moore, C.; Nicinski, T.; Oleynik, G.; Pordes, R.; Sergey, G.; Votava, M.; White, V.

    1989-05-01

    The Online and Data Acquisition software groups at Fermi National Accelerator Laboratory have extended the VAXONLINE data acquisition package to include a VME based data path. The resulting environment, PAN-DA, provides a high throughput for logging, filtering, formatting and selecting events. 10 refs., 1 fig.

  17. Hidden sketches by Leonardo da Vinci revealed

    NASA Astrophysics Data System (ADS)

    Dumé, Belle

    2009-02-01

    Three drawings on the back of Leonardo da Vinci's The Virgin and Child with St Anne (circa 1508) have been discovered by researchers led by Michel Menu from the Centre de Recherche et de Restauration des Musées de France (C2RMF) and the Louvre Museum in Paris.

  18. How to Think Like Leonardo da Vinci

    ERIC Educational Resources Information Center

    Caouette, Ralph

    2008-01-01

    To be effective and relevant in twenty-first-century learning, art needs to be more inclusive. In this article, the author discusses how teachers can find a good example in Leonardo da Vinci for building an art program. His art, design, and curiosity are the perfect foundation for any art program, at any level. (Contains 3 resources and 3 online…

  19. 40 CFR 60.51Da - Reporting requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Generating Units § 60.51Da Reporting requirements. (a) For SO2, NOX, PM, and NOX plus CO emissions, the... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Reporting requirements. 60.51Da Section 60.51Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS...

  20. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Commercial demonstration permit. 60.47Da Section 60.47Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... Steam Generating Units § 60.47Da Commercial demonstration permit. (a) An owner or operator of...

  1. 40 CFR 60.51Da - Reporting requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Generating Units § 60.51Da Reporting requirements. (a) For SO2, NOX, PM, and NOX plus CO emissions, the... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Reporting requirements. 60.51Da Section 60.51Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS...

  2. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Commercial demonstration permit. 60.47Da Section 60.47Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... Steam Generating Units § 60.47Da Commercial demonstration permit. (a) An owner or operator of...

  3. 40 CFR 60.51Da - Reporting requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Generating Units § 60.51Da Reporting requirements. (a) For SO2, NOX, PM, and NOX plus CO emissions, the... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Reporting requirements. 60.51Da Section 60.51Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS...

  4. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Commercial demonstration permit. 60.47Da Section 60.47Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... Steam Generating Units § 60.47Da Commercial demonstration permit. (a) An owner or operator of...

  5. 32 CFR 516.25 - DA Form 4.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Reporting Legal Proceedings to HQDA § 516.25 DA Form 4. (a) General. The DA Form 4 (See figure...

  6. 32 CFR 516.25 - DA Form 4.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Reporting Legal Proceedings to HQDA § 516.25 DA Form 4. (a) General. The DA Form 4 (See figure...

  7. A Day in the Life at DaVita Academy

    ERIC Educational Resources Information Center

    Weinstein, Margery

    2010-01-01

    When a company name means "giving life," the bar for learning and development programs is held high. In this article, the author describes what it takes to graduate from DaVita Academy, the soft skills training program dialysis services company DaVita offers all its employees. DaVita's chief executive officer, Kent Thiry, states that the Academy…

  8. The DA{phi}NE-Light Facility

    SciTech Connect

    Burattini, Emilio; Cinque, Gianfelice; Dabagov, Sultan; Grilli, Antonio; Marcelli, Augusto; Pace, Elisabetta; Piccinini, Massimo; Raco, Agostino; Monti, Francesca

    2004-05-12

    The new Synchrotron Radiation facility at Frascati exploits the intense photon emission from DA{phi}NE, the 0,51 GeV storage ring circulating over 1 A of electrons. Among the three beamlines commissioned, the Synchrotron INfrared Beamline At Da{phi}ne (SINBAD) is fully operational by a brilliant SR beam spanning the entire IR. Recently, the soft X-ray beamline has been characterized and, once implemented the double-crystal monocromator, X-ray Absorption Spectroscopy is applied on material standards in the distinguishing energy region below 4 keV. An UltraViolet line, presently dedicated to photobiology dosimetry, has also given first results on cell irradiation in the UVB band.

  9. The Real Code of Leonardo da Vinci

    PubMed Central

    Ose, Leiv

    2008-01-01

    Leonardo da Vinci was born in Italy. Among the researchers and scientists, he is favourably known for his remarkable efforts in scientific work. His investigations of atherosclerosis judiciously combine three separate fields of research. In 1506, he finished his masterpiece, painting of Mona Lisa. A careful clinical examination of the famous painting reveals a yellow irregular leather-like spot at the inner end of the left upper eyelid and a soft bumpy well-defined swelling of the dorsum of the right hand beneath the index finger about 3 cm long. This is probably the first case of familial hypercholesterolemia (FH). The FH code of Leonardo da Vinci was given immense consideration by scientists like Carl Muller, who described the xanthomas tuberosum and angina pectoris. On the contrary, Akira Endo searched for microbial metabolites that would inhibit HMG-CoA reductase, the rate-limiting enzyme in the synthesis of cholesterol and finally, Michael Brown and Joseph Goldstein published a remarkable series of elegant and insightful papers in the 70s and 80s. They established that the cellular uptake of low-density lipoprotein (LDL) essentially requires the LDL receptor. In conclusion: this was the real Code of Leonardo da Vinci. PMID:19924278

  10. 40 CFR 60.52Da - Recordkeeping requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Recordkeeping requirements. 60.52Da Section 60.52Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Electric Utility Steam Generating Units for...

  11. Asteroid 2012 DA14 To Whiz Past Earth Safely

    NASA Video Gallery

    The small near-Earth asteroid 2012 DA14 will pass very close to Earth on Feb. 15, 2013. Asteroid 2012 DA14 will be closest to Earth at about 11:24 a.m. PST (2:24 p.m. EST and 1924 UTC), on Feb. 15,...

  12. The Case: Bunche-Da Vinci Learning Partnership Academy

    ERIC Educational Resources Information Center

    Eisenberg, Nicole; Winters, Lynn; Alkin, Marvin C.

    2005-01-01

    The Bunche-Da Vinci case described in this article presents a situation at Bunche Elementary School that four theorists were asked to address in their evaluation designs (see EJ791771, EJ719772, EJ791773, and EJ792694). The Bunche-Da Vinci Learning Partnership Academy, an elementary school located between an urban port city and a historically…

  13. Da Costa's syndrome or neurocirculatory asthenia.

    PubMed Central

    Paul, O

    1987-01-01

    The syndrome variously called Da Costa's syndrome, effort syndrome, neurocirculatory asthenia, etc has been studied for more than 100 years by many distinguished physicians. Originally identified in men in wartime, it has been widely recognised as a common chronic condition in both sexes in civilian life. Although the symptoms may seem to appear after infections and various physical and psychological stresses, neurocirculatory asthenia is most often encountered as a familial disorder that is unrelated to these factors, although they may aggravate an existing tendency. Respiratory complaints (including breathlessness, with and without effort, and smothering sensations) are almost universal, and palpitation, chest discomfort, dizziness and faintness, and fatigue are common. The physical examination is normal. The aetiology is obscure but patients usually have a normal life span. Reassurance and measures to improve physical fitness are helpful. PMID:3314950

  14. 75 FR 52292 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Diamond Aircraft... new airworthiness directive (AD) for all Diamond Aircraft Industries GmbH Models DA 40 and DA...

  15. 75 FR 75868 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-07

    ... Register on August 25, 2010 (75 FR 52292). That NPRM proposed to require a retrofit of the rear passenger... 12866, (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034... Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes AGENCY: Federal Aviation Administration,...

  16. Metal Lines in DA White Dwarfs

    NASA Astrophysics Data System (ADS)

    Zuckerman, B.; Koester, D.; Reid, I. N.; Hünsch, M.

    2003-10-01

    We report Keck telescope HIRES echelle observations of DA white dwarfs in a continuation of an extensive search for metals. These spectra are supplemented with new JHK magnitudes that are used to determine improved atmospheric parameters. Of the DA white dwarfs not in binary or common proper motion systems, about 25% show Ca II lines. For these, Ca abundances are determined from comparison with theoretical equivalent widths from model atmosphere calculations; in a few cases we also obtain Mg, Fe, Si, and Al abundances. If Ca is not observed, we generally determine very stringent upper limits. We compare the data to predictions of previously published models involving the accretion/diffusion of interstellar matter and of comets. The derived abundances are not obviously compatible with the predictions of either model, which up to now could only be tested with traces of metals in helium-rich white dwarfs. By modifying certain assumptions in the published interstellar accretion model we are able to match the distribution of the elements in the white dwarf atmospheres, but, even so, tests of other expectations from this scenario are less successful. Because comet accretion appears unlikely to be the primary cause of the DAZ phenomenon, the data suggest that no more than about 20% of F-type main-sequence stars are accompanied by Oort-like comet clouds. This represents the first observational estimate of this fraction. A plausible alternative to the accretion of cometary or interstellar matter is disruption and accretion of asteroidal material, a model first suggested in 1990 to explain excess near-infrared emission from the DAZ G29-38. An asteroidal debris model to account for the general DAZ phenomenon does not presently disagree with the HIRES data, but neither is there any compelling evidence in support of such a model. The HIRES data indicate that in close red dwarf/white dwarf binaries not known to be cataclysmic variables there is, nonetheless, significant mass

  17. Der Telemanipulator daVinci als mechanisches Trackingsystem

    NASA Astrophysics Data System (ADS)

    Käst, Johannes; Neuhaus, Jochen; Nickel, Felix; Kenngott, Hannes; Engel, Markus; Short, Elaine; Reiter, Michael; Meinzer, Hans-Peter; Maier-Hein, Lena

    Der Telemanipulator daVinci (Intuitive Surgical, Sunnyvale, Kalifornien) ist ein M aster-Slave System für roboterassistierte minimalinvasive Chirurgie. Da er über integrierte Gelenksensoren verfügt, kann er unter Verwendung der daVinci-API als mechanisches Trackingsystem verwendet werden. In dieser Arbeit evaluieren wir die Präzision und Genauigkeit eines daVinci mit Hilfe eines Genauigkeitsphantoms mit bekannten Maßen. Der ermittelte Positionierungsfehler liegt in der Größenordnung von 6 mm und ist somit für einen Großteil der medizinischen Fragestellungen zu hoch. Zur Reduktion des Fehlers schlagen wir daher eine Kalibrierung der Gelenksensoren vor.

  18. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... affected unit that qualifies as a gas-fired or oil-fired unit, as defined in 40 CFR 72.2, may use, as an... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Emission monitoring. 60.49Da Section 60... Generating Units § 60.49Da Emission monitoring. (a) An owner or operator of an affected facility subject...

  19. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... affected unit that qualifies as a gas-fired or oil-fired unit, as defined in 40 CFR 72.2, may use, as an... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Emission monitoring. 60.49Da Section 60... Generating Units for Which Construction is Commenced After September 18, 1978 § 60.49Da Emission...

  20. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... affected unit that qualifies as a gas-fired or oil-fired unit, as defined in 40 CFR 72.2, may use, as an... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Emission monitoring. 60.49Da Section 60... Generating Units § 60.49Da Emission monitoring. (a) An owner or operator of an affected facility subject...

  1. Tree Branching: Leonardo da Vinci's Rule versus Biomechanical Models

    PubMed Central

    Minamino, Ryoko; Tateno, Masaki

    2014-01-01

    This study examined Leonardo da Vinci's rule (i.e., the sum of the cross-sectional area of all tree branches above a branching point at any height is equal to the cross-sectional area of the trunk or the branch immediately below the branching point) using simulations based on two biomechanical models: the uniform stress and elastic similarity models. Model calculations of the daughter/mother ratio (i.e., the ratio of the total cross-sectional area of the daughter branches to the cross-sectional area of the mother branch at the branching point) showed that both biomechanical models agreed with da Vinci's rule when the branching angles of daughter branches and the weights of lateral daughter branches were small; however, the models deviated from da Vinci's rule as the weights and/or the branching angles of lateral daughter branches increased. The calculated values of the two models were largely similar but differed in some ways. Field measurements of Fagus crenata and Abies homolepis also fit this trend, wherein models deviated from da Vinci's rule with increasing relative weights of lateral daughter branches. However, this deviation was small for a branching pattern in nature, where empirical measurements were taken under realistic measurement conditions; thus, da Vinci's rule did not critically contradict the biomechanical models in the case of real branching patterns, though the model calculations described the contradiction between da Vinci's rule and the biomechanical models. The field data for Fagus crenata fit the uniform stress model best, indicating that stress uniformity is the key constraint of branch morphology in Fagus crenata rather than elastic similarity or da Vinci's rule. On the other hand, mechanical constraints are not necessarily significant in the morphology of Abies homolepis branches, depending on the number of daughter branches. Rather, these branches were often in agreement with da Vinci's rule. PMID:24714065

  2. Migration of ATLAS PanDA to CERN

    NASA Astrophysics Data System (ADS)

    Stewart, Graeme Andrew; Klimentov, Alexei; Koblitz, Birger; Lamanna, Massimo; Maeno, Tadashi; Nevski, Pavel; Nowak, Marcin; Emanuel De Castro Faria Salgado, Pedro; Wenaus, Torre

    2010-04-01

    The ATLAS Production and Distributed Analysis System (PanDA) is a key component of the ATLAS distributed computing infrastructure. All ATLAS production jobs, and a substantial amount of user and group analysis jobs, pass through the PanDA system, which manages their execution on the grid. PanDA also plays a key role in production task definition and the data set replication request system. PanDA has recently been migrated from Brookhaven National Laboratory (BNL) to the European Organization for Nuclear Research (CERN), a process we describe here. We discuss how the new infrastructure for PanDA, which relies heavily on services provided by CERN IT, was introduced in order to make the service as reliable as possible and to allow it to be scaled to ATLAS's increasing need for distributed computing. The migration involved changing the backend database for PanDA from MySQL to Oracle, which impacted upon the database schemas. The process by which the client code was optimised for the new database backend is discussed. We describe the procedure by which the new database infrastructure was tested and commissioned for production use. Operations during the migration had to be planned carefully to minimise disruption to ongoing ATLAS offline computing. All parts of the migration were fully tested before commissioning the new infrastructure and the gradual migration of computing resources to the new system allowed any problems of scaling to be addressed.

  3. PKC phosphorylates residues in the N-terminal of the DA transporter to regulate amphetamine-induced DA efflux.

    PubMed

    Wang, Qiang; Bubula, Nancy; Brown, Jason; Wang, Yunliang; Kondev, Veronika; Vezina, Paul

    2016-05-27

    The DA transporter (DAT), a phosphoprotein, controls extracellular dopamine (DA) levels in the central nervous system through transport or reverse transport (efflux). Multiple lines of evidence support the claim that PKC significantly contributes to amphetamine-induced DA efflux. Other signaling pathways, involving CaMKII and ERK, have also been shown to regulate DAT mediated efflux. Here we assessed the contribution of putative PKC residues (S4, S7, S13) in the N-terminal of the DAT to amphetamine-induced DA efflux by transfecting DATs containing different serine to alanine (S-A) point mutations into DA pre-loaded HEK-293 cells and incubating these cells in amphetamine (2μM). The effects of a S-A mutation at the non-PKC residue S12 and a threonine to alanine (T-A) mutation at the ERK T53 residue were also assessed for comparison. WT-DATs were used as controls. In an initial experiment, we confirmed that inhibiting PKC with Go6976 (130nM) significantly reduced amphetamine-induced DA efflux. In subsequent experiments, cells transfected with the S4A, S12A, S13A, T53A and S4,7,13A mutants showed a reduction in amphetamine-induced DA efflux similar to that observed with Go6976. Interestingly, cells transfected with the S7A mutant, identified by some as a PKC-PKA residue, showed unperturbed WT-DAT levels of amphetamine-induced DA efflux. These results indicate that phosphorylation by PKC of select residues in the DAT N-terminal can regulate amphetamine-induced efflux. PKC can act either independently or in concert with other kinases such as ERK to produce this effect. PMID:27113203

  4. A cosmologia no ensino da geografia

    NASA Astrophysics Data System (ADS)

    Santos, S. C.; Chiaradia, A. P. M.

    2003-08-01

    O principal objetivo deste trabalho é auxiliar o professor de Geografia em sala de aula no ensino de tópicos relacionados com a Cosmologia. A idéia deste trabalho surgiu quando foi constatado que o professor de Geografia tem dificuldades de ensinar este tópico. Esta constatação foi feita por uma das autoras ao lecionar este tópico no ensino fundamental e em discussões com outros professores de Geografia. Da mesma maneira que ocorria desde os tempos mais antigos, os alunos têm muito interesse em conhecer os fenômenos que ocorrem no Cosmo, porém os livros didáticos de Geografia utilizados em sala de aula não são ricos em informações sobre este assunto. Assim, o professor de Geografia tem poucas informações para discutir este assunto em sala de aula e não dá a devida importância para este tópico. Então, foi desenvolvido um material de apoio para professores de Geografia sobre a origem do Universo, sua evolução e seu possível futuro evolutivo segundo as mais recentes teorias, com base em perguntas feitas pelos alunos de ensino fundamental e as informações trazidas nos livros didáticos Não cabe a este material inovar e tão pouco trazer uma metodologia de ensino de Cosmologia. Neste material o professor de Geografia pode encontrará um banco de informações, que constitui no estabelecimento de conceitos, teorias e hipóteses, sobre a Cosmologia, em linguagem simples e de fácil entendimento. Para desenvolvê-lo, foram feitas pesquisas não exaustivas em livros e revistas científicas, compilação e discussão em forma cronológica das teorias aceitas sobre modelos cosmológicos. Portanto, este material será apresentado neste trabalho.

  5. Overview of ATLAS PanDA Workload Management

    SciTech Connect

    Maeno T.; De K.; Wenaus T.; Nilsson P.; Stewart G. A.; Walker R.; Stradling A.; Caballero J.; Potekhin M.; Smith D.

    2011-01-01

    The Production and Distributed Analysis System (PanDA) plays a key role in the ATLAS distributed computing infrastructure. All ATLAS Monte-Carlo simulation and data reprocessing jobs pass through the PanDA system. We will describe how PanDA manages job execution on the grid using dynamic resource estimation and data replication together with intelligent brokerage in order to meet the scaling and automation requirements of ATLAS distributed computing. PanDA is also the primary ATLAS system for processing user and group analysis jobs, bringing further requirements for quick, flexible adaptation to the rapidly evolving analysis use cases of the early datataking phase, in addition to the high reliability, robustness and usability needed to provide efficient and transparent utilization of the grid for analysis users. We will describe how PanDA meets ATLAS requirements, the evolution of the system in light of operational experience, how the system has performed during the first LHC data-taking phase and plans for the future.

  6. The ATLAS PanDA Monitoring System and its Evolution

    NASA Astrophysics Data System (ADS)

    Klimentov, A.; Nevski, P.; Potekhin, M.; Wenaus, T.

    2011-12-01

    The PanDA (Production and Distributed Analysis) Workload Management System is used for ATLAS distributed production and analysis worldwide. The needs of ATLAS global computing imposed challenging requirements on the design of PanDA in areas such as scalability, robustness, automation, diagnostics, and usability for both production shifters and analysis users. Through a system-wide job database, the PanDA monitor provides a comprehensive and coherent view of the system and job execution, from high level summaries to detailed drill-down job diagnostics. It is (like the rest of PanDA) an Apache-based Python application backed by Oracle. The presentation layer is HTML code generated on the fly in the Python application which is also responsible for managing database queries. However, this approach is lacking in user interface flexibility, simplicity of communication with external systems, and ease of maintenance. A decision was therefore made to migrate the PanDA monitor server to Django Web Application Framework and apply JSON/AJAX technology in the browser front end. This allows us to greatly reduce the amount of application code, separate data preparation from presentation, leverage open source for tools such as authentication and authorization mechanisms, and provide a richer and more dynamic user experience. We describe our approach, design and initial experience with the migration process.

  7. Overview of ATLAS PanDA Workload Management

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Wenaus, T.; Nilsson, P.; Stewart, G. A.; Walker, R.; Stradling, A.; Caballero, J.; Potekhin, M.; Smith, D.; ATLAS Collaboration

    2011-12-01

    The Production and Distributed Analysis System (PanDA) plays a key role in the ATLAS distributed computing infrastructure. All ATLAS Monte-Carlo simulation and data reprocessing jobs pass through the PanDA system. We will describe how PanDA manages job execution on the grid using dynamic resource estimation and data replication together with intelligent brokerage in order to meet the scaling and automation requirements of ATLAS distributed computing. PanDA is also the primary ATLAS system for processing user and group analysis jobs, bringing further requirements for quick, flexible adaptation to the rapidly evolving analysis use cases of the early datataking phase, in addition to the high reliability, robustness and usability needed to provide efficient and transparent utilization of the grid for analysis users. We will describe how PanDA meets ATLAS requirements, the evolution of the system in light of operational experience, how the system has performed during the first LHC data-taking phase and plans for the future.

  8. 77 FR 39725 - Land Acquisitions: Pueblo of Santo Domingo

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ...: Douglas Hickman, Realty Officer, Southern Pueblos Agency, Bureau of Indian Affairs, 1001 Indian School... T. 17 N., R. 4 E., Sec. 25, lots 1 to 4, inclusive, and S\\1/2\\S\\1/2\\; Sec. 26, lots 1 to 4, inclusive, and S\\1/2\\S\\1/2\\; Sec. 27, lots 1 to 4, inclusive, and S\\1/2\\S\\1/2\\; Sec. 28, lots 1 to...

  9. Geomagnetic observations on tristan da cunha, south atlantic ocean

    USGS Publications Warehouse

    Matzka, J.; Olsen, N.; Maule, C.F.; Pedersen, L.W.; Berarducci, A.M.; Macmillan, S.

    2009-01-01

    Few geomagnetic ground observations exist of the Earth's strongest core field anomaly, the South Atlantic Anomaly (SAA). The geomagnetic repeat station on the island Tristan da Cunha, located half-way between South Africa and South America at 37?? 05' S, 12?? 18' W, is therefore of crucial importance. We have conducted several sets of repeat station measurements during magnetically quiet conditions (Kp 2o or less) in 2004. The procedures are described and the results are compared to those from earlier campaigns and to the predictions of various global field models. Features of the local crustal bias field and the solar quiet daily variation are discussed. We also evaluate the benefit of continuous magnetic field recordings from Tristan da Cunha, and argue that such a data set is a very valuable addition to geomagnetic satellite data. Recently, funds were set up to establish and operate a magnetometer station on Tristan da Cunha during the Swarm magnetic satellite mission (2011-2014).

  10. Recent Improvements in the ATLAS PanDA Pilot

    NASA Astrophysics Data System (ADS)

    Nilsson, P.; Caballero Bejar, J.; Compostella, G.; Contreras, C.; De, K.; Dos Santos, T.; Maeno, T.; Potekhin, M.; Wenaus, T.

    2012-12-01

    The Production and Distributed Analysis system (PanDA) in the ATLAS experiment uses pilots to execute submitted jobs on the worker nodes. The pilots are designed to deal with different runtime conditions and failure scenarios, and support many storage systems. This talk will give a brief overview of the PanDA pilot system and will present major features and recent improvements including CernVM File System integration, the job retry mechanism, advanced job monitoring including JEM technology, and validation of new pilot code using the HammerCloud stress-testing system. PanDA is used for all ATLAS distributed production and is the primary system for distributed analysis. It is currently used at over 130 sites worldwide. We analyze the performance of the pilot system in processing LHC data on the OSG, EGI and Nordugrid infrastructures used by ATLAS, and describe plans for its further evolution.

  11. Situação da Mulher na Astronomia Brasileira

    NASA Astrophysics Data System (ADS)

    Silva, Adriana V. R.

    2007-07-01

    O conteúdo desse texto surgiu de uma apresentação de mesmo título que fiz na XXXI Reunião Anual da Sociedade Astronômica Brasileira (SAB) em 2005. Esse tema foi inspirado originalmente pela minha participação no "2nd UIPAP International Conference on Women in Physics" realizado entre 23 e 25 de maio de 2005 no Rio de Janeiro. Essa é uma conferência internacional que acontece de três em três anos, sendo que a primeira ocorreu em 2002 na cidade de Paris, França. Participei dessa conferência como membro da delegação da Sociedade Brasileira de Física e um dos trabalhos que apresentei versava sobre a situação das mulheres na Astronomia brasileira, cujos resultados principais discorro a seguir. A situação das astrônomas, baseada nos dados dos sócios da SAB coletados no final de 2004, é comparada com a das físicas brasileiras e também com as nossas colegas americanas. Os dados identificam ainda uma maior evasão da carreira por parte das mulheres do que os homens. Alguns dos possíveis motivos da evasão são discutidos, como o desejo de constituir família e/ou isolamento. Resultados um tanto preocupantes com relação à distribuição de bolsas de produtividade do CNPq também são apresentados. As principais discussões e estratégias recomendadas nesse congresso são mencionadas de forma resumida ao final.

  12. The future of PanDA in ATLAS distributed computing

    NASA Astrophysics Data System (ADS)

    De, K.; Klimentov, A.; Maeno, T.; Nilsson, P.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.

    2015-12-01

    Experiments at the Large Hadron Collider (LHC) face unprecedented computing challenges. Heterogeneous resources are distributed worldwide at hundreds of sites, thousands of physicists analyse the data remotely, the volume of processed data is beyond the exabyte scale, while data processing requires more than a few billion hours of computing usage per year. The PanDA (Production and Distributed Analysis) system was developed to meet the scale and complexity of LHC distributed computing for the ATLAS experiment. In the process, the old batch job paradigm of locally managed computing in HEP was discarded in favour of a far more automated, flexible and scalable model. The success of PanDA in ATLAS is leading to widespread adoption and testing by other experiments. PanDA is the first exascale workload management system in HEP, already operating at more than a million computing jobs per day, and processing over an exabyte of data in 2013. There are many new challenges that PanDA will face in the near future, in addition to new challenges of scale, heterogeneity and increasing user base. PanDA will need to handle rapidly changing computing infrastructure, will require factorization of code for easier deployment, will need to incorporate additional information sources including network metrics in decision making, be able to control network circuits, handle dynamically sized workload processing, provide improved visualization, and face many other challenges. In this talk we will focus on the new features, planned or recently implemented, that are relevant to the next decade of distributed computing workload management using PanDA.

  13. The role of transparency in da Vinci stereopsis.

    PubMed

    Zannoli, Marina; Mamassian, Pascal

    2011-10-15

    The majority of natural scenes contains zones that are visible to one eye only. Past studies have shown that these monocular regions can be seen at a precise depth even though there are no binocular disparities that uniquely constrain their locations in depth. In the so-called da Vinci stereopsis configuration, the monocular region is a vertical line placed next to a binocular rectangular occluder. The opacity of the occluder has been mentioned to be a necessary condition to obtain da Vinci stereopsis. However, this opacity constraint has never been empirically tested. In the present study, we tested whether da Vinci stereopsis and perceptual transparency can interact using a classical da Vinci configuration in which the opacity of the occluder varied. We used two different monocular objects: a line and a disk. We found no effect of the opacity of the occluder on the perceived depth of the monocular object. A careful analysis of the distribution of perceived depth revealed that the monocular object was perceived at a depth that increased with the distance between the object and the occluder. The analysis of the skewness of the distributions was not consistent with a double fusion explanation, favoring an implication of occlusion geometry in da Vinci stereopsis. A simple model that includes the geometry of the scene could account for the results. In summary, the mechanism responsible to locate monocular regions in depth is not sensitive to the material properties of objects, suggesting that da Vinci stereopsis is solved at relatively early stages of disparity processing. PMID:21906614

  14. Publishing Planetary Data to the VO using DaCHS

    NASA Astrophysics Data System (ADS)

    Demleitner, M.

    2014-04-01

    Publishing to the Virtual Observatory has many advantages, but between standards compliance, registration, data organisation, and server operation it is also requires a nontrivial effort if starting from scratch. The DaCHS software from the German Astrophysical Virtual Observatory offers a packaged solution letting publishers concentrate on working with their own data and metadata as much as possible. For the planetary community, publishing is further facilitated by built-in support for the EPN-TAP data model. This contribution will show a typical publishing workflow together with a brief overview of how DaCHS meshes in with the VO and Web-based data usage.

  15. Asteroid 1986 DA - Radar evidence for a metallic composition

    NASA Technical Reports Server (NTRS)

    Ostro, S. J.; Rosema, K. D.; Campbell, D. B.; Chandler, J. F.; Hine, A. A.; Hudson, R. S.

    1991-01-01

    Echoes from the near-earth object 1986 DA show it to be significantly more reflective than other radar-detected asteroids. This result supports the hypothesis that 1986 DA is a piece of NiFe metal derived from the interior of a much larger object that melted, differentiated, cooled, and subsequently was disrupted in a catastrophic collision. This 2-kilometer asteroid, which appears smooth at centimeter to meter scales but extremely irregular at 10- to 100-meter scales, might be (or have been a part of) the parent body of some iron meteorites.

  16. Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II.

    PubMed

    Tohri, Akihiko; Dohmae, Naoshi; Suzuki, Takehiro; Ohta, Hisataka; Inoue, Yasunori; Enami, Isao

    2004-03-01

    To elucidate the domains on the extrinsic 23 kDa protein involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II, we modified amino or carboxyl groups of the 23 kDa protein to uncharged methyl ester groups with N-succinimidyl propionate or glycine methyl ester in the presence of a water-soluble carbodiimide, respectively. The N-succinimidyl propionate-modified 23 kDa protein did not bind to the 33 kDa protein associated with PSII membranes, whereas the glycine methyl ester-modified 23 kDa protein completely bound. This indicates that positive charges on the 23 kDa protein are important for electrostatic interaction with the 33 kDa protein associated with the PSII membranes. Mapping of the N-succinimidyl propionate-modified sites of the 23 kDa protein was performed using Staphylococcus V8 protease digestion of the modified protein followed by determination of the mass of the resultant peptide fragments with MALDI-TOF MS. The results showed that six domains (Lys11-Lys14, Lys27-Lys38, Lys40, Lys90-Lys96, Lys143-Lys152, Lys166-Lys174) were modified with N-succinimidyl propionate. In these domains, Lys11, Lys13, Lys33, Lys38, Lys143, Lys166, Lys170 and Lys174 were wholly conserved in the 23 kDa protein from 12 species of higher plants. These positively charged lysyl residues on the 23 kDa protein may be involved in electrostatic interactions with the negatively charged carboxyl groups on the 33 kDa protein, the latter has been suggested to be important for the 23 kDa binding [Bricker, T.M. & Frankel, L.K. (2003) Biochemistry42, 2056-2061]. PMID:15009208

  17. DATA ASSESSMENT REPORTS FOR CEMS AT SUBPART DA FACILITIES

    EPA Science Inventory

    EPA promulgated minimum quality assurance (QA) requirements for Continuous Emission Monitoring Systems (CEMS) in 40 CFR Part 60 Appendix F. Appendix F requires Da source owners to develop site-specific QA plans and report the results of EPA specified QA activities each calendar q...

  18. Asteroid 1986 DA: Radar evidence for a metallic composition

    NASA Technical Reports Server (NTRS)

    Ostro, Steven J.; Campbell, D. B.; Chandler, J. F.; Hine, A. A.; Hudson, R. S.; Rosema, K. D.; Shapiro, Irwin I.

    1991-01-01

    Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece of NiFe metal derived from the interior of a much larger object that melted, differentiated, cooled and subsequently was disrupted in a catastrophic collision. This 2-km asteroid might be (or have been part of) the parent body of some iron meteorites. Or 1986 DA might share the parentage and/or part of the dynamical history of some meteorites without ever having contributed any of its own ejecta to our meteorite sample. Analysis of the samples returned from 1986 DA might ultimately involve economic considerations. Meteoritic metal is mostly iron with about 8 percent nickel, but also contains substantial concentrations of precious and strategic metals, including approx. 1 ppm of gold and approx. 10 ppm of platinum group elements. If these abundances apply to 1986 DA, it contains some 10(exp 16) g of iron, 10 (exp 15) g of nickel, 10(exp 11) g of platinum group metals, and 10(exp 10) g of gold.

  19. 40 CFR 60.45Da - Standard for mercury (Hg).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Standard for mercury (Hg). 60.45Da... for mercury (Hg). (a) For each coal-fired electric utility steam generating unit other than an IGCC... gases that contain mercury (Hg) emissions in excess of each Hg emissions limit in paragraphs...

  20. Training and Health. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles programs in the fields of health and medicine that are offered through the European Commission's Leonardo da Vinci program. The following programs are profiled: (1) CYTOTRAIN (a transnational vocational training program in cervical cancer screening); (2) Apollo (a program of open and distance learning for paramedical…

  1. The DaVinci Project: Multimedia in Art and Chemistry.

    ERIC Educational Resources Information Center

    Simonson, Michael; Schlosser, Charles

    1998-01-01

    Provides an overview of the DaVinci Project, a collaboration of students, teachers, and researchers in chemistry and art to develop multimedia materials for grades 3-12 visualizing basic concepts in chemistry and visual art. Topics addressed include standards in art and science; the conceptual framework for the project; and project goals,…

  2. The Potential da Vinci in All of Us

    ERIC Educational Resources Information Center

    Petto, Sarah; Petto, Andrew

    2009-01-01

    The study of the human form is fundamental to both science and art curricula. For vertebrates, perhaps no feature is more important than the skeleton to determine observable form and function. As Leonard da Vinci's famous Proportions of the Human Figure (Virtruvian Man) illustrates, the size, shape, and proportions of the human body are defined by…

  3. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... potential combustion concentration (80 percent reduction) for each 24-hour period of steam generator... owner or operator of a fluidized bed combustion electric utility steam generator (atmospheric or... Steam Generating Units for Which Construction is Commenced After September 18, 1978 § 60.47Da...

  4. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... potential combustion concentration (80 percent reduction) for each 24-hour period of steam generator... owner or operator of a fluidized bed combustion electric utility steam generator (atmospheric or... Steam Generating Units for Which Construction is Commenced After September 18, 1978 § 60.47Da...

  5. Women and Technical Professions. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles programs for women in technical professions that are offered through the European Commission's Leonardo da Vinci program. The following programs are profiled: (1) Artemis and Diana (vocational guidance programs to help direct girls toward technology-related careers); (2) CEEWIT (an Internet-based information and…

  6. Studying and Working Abroad. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles recent successful examples of students studying and working abroad as part of the European Commission's Leonardo da Vinci program, which is designed to give students across the European Union the opportunity to experience vocational training in a foreign country. The following examples are presented: (1) 3 Finnish students…

  7. Modelagem do vento e da fotosfera de AG Carinae

    NASA Astrophysics Data System (ADS)

    Groh, J. H.; Damineli, A.

    2003-08-01

    A trajetória evolutiva das estrelas de alta massa depende fortemente de suas taxas de perda de massa. Apesar do rápido progresso no estudo destas estrelas, a taxa de perda de massa e outros parâmetros físicos básicos, como a temperatura superficial e a velocidade terminal do vento ainda não estão bem determinados. Isto ocorre devido à presença de ventos irregulares, rápidos e fortes ao redor destas estrelas, tornando a interpretação dos seus espectros uma tarefa difícil. Assim, a modelagem do vento e da fotosfera dessas estrelas está sendo cada vez mais usada para obter tais parâmetros a partir dos espectros. O aumento da taxa de perda de massa durante a fase LBV (Variáveis Luminosas Azuis), comparado com outros tipos de estrelas, tem sido atribuído a instabilidades do tipo S Doradus. Dispomos de uma base de dados espectroscópicos cobrindo 22 anos de observações de AG Carinae, incluindo um ciclo S Doradus completo, com espectros CCD em alta resolução na faixa óptica e infravermelha. Utilizamos o programa desenvolvido por Schmutz (1997) para uma análise preliminar desse ciclo, obtendo a taxa de perda de massa a partir da linha do Ha. Não existe uma correlação clara da taxa de perda de massa com mudanças da temperatura efetiva, do raio da estrela e do fluxo na banda V. A estrela atingiu seu mínimo fotométrico (raio mínimo) em 1990 e o máximo fotométrico (raio máximo) em 1995, enquanto que o fluxo máximo da linha do Ha ocorreu em 1996. Além disso a taxa de perda de massa não segue esse ciclo, contrariamente às idéias correntes. Para fazer um modelo mais realista estamos usando o programa CMFGEN (Hillier & Miller), que trata a fotosfera e o vento estelar de forma consistente, considerando a radiação fora do equilíbrio termodinâmico (NLTE) e com blanketting total de linhas. Simulamos o espectro de AG Carinae em duas épocas extremas do ciclo S Dor para testar os resultados obtidos com o modelo mais simplificado.

  8. Roles and Delegation of Authority (R/DA) System

    SciTech Connect

    ABBOTT,JOHN P.; HUTCHINS,JAMES C.; SCHOCH,DAVID G.

    1999-11-01

    The processes of defining managerial roles and providing for delegation of authority are essential to any enterprise. At most large organizations, these processes are defined in policy manuals and through sets of standard operating procedures for many, if not all, business and administrative functions. Many of these staff-initiated, administrative functions require the routing of documents for approval to one or more levels of management. These employee-oriented, back office types of workflows tend to require more flexibility in determining to whom these documents should go to, while, at the same time, providing the responsible parties with the flexibility to delegate their approval authority or allow others to review their work. Although this practice is commonplace in manual, paper-based processes that exist in many organizations, it is difficult to provide the same flexibility in the more structured, electronic-based, workflow systems. The purpose of this report is to present a framework or architecture for creating a R/DA system and provide some insights associated with its design and utilization. To improve understanding and clarify subsequent discussion, the goals and requirements for the major R/DA system components, namely, the database and interface modules, are initially presented along with the identification of important concepts and the definition of critical terms. Next high-level functions relating the types of inputs to the outputs of the R/DA interface module are introduced and discussed. Then the relationships between the major R/DA modules and the primary components associated with its creation and maintenance are presented and analyzed. Finally, some conclusions are drawn relative to the advantages associated with developing a R/DA system for use in implementing an enterprise-wide, work-facilitating information system.

  9. 49 CFR 178.58 - Specification 4DA welded steel cylinders for aircraft use.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Specification 4DA welded steel cylinders for...) SPECIFICATIONS FOR PACKAGINGS Specifications for Cylinders § 178.58 Specification 4DA welded steel cylinders for aircraft use. (a) Type, size, and service pressure. A DOT 4DA is a welded steel sphere (two...

  10. 49 CFR 178.58 - Specification 4DA welded steel cylinders for aircraft use.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Specification 4DA welded steel cylinders for...) SPECIFICATIONS FOR PACKAGINGS Specifications for Cylinders § 178.58 Specification 4DA welded steel cylinders for aircraft use. (a) Type, size, and service pressure. A DOT 4DA is a welded steel sphere (two...

  11. In vivo breakdown products of the 32 kDa thylakoid herbicide binding protein. [Spirodela oligorrhiza

    SciTech Connect

    Greenberg, B.M.; Mattoo, A.K.; Gaba, V.; Edelman, M.

    1986-04-01

    The 32 kDa herbicide binding protein of PSII is degraded rapidly in the light. This phenomenon was investigated in Spirodela oligorrhiza. When fronds were radiolabeled in the presence of cycloheximide only the chloroplast-encoded proteins were labeled. Under these conditions, a breakdown product of 23.5 kDa was observed. This polypeptide was further degraded with kinetics similar to that of the 32 kDa protein. The 23.5 kDa polypeptide cross-reacted with an antibody specific to the 32 kDa protein. By protease digestion it was determined that the 23.5 kDa polypeptide is the intact N-terminal piece of the 32 kDa protein. Thus, this product corresponds to the membrane anchor of the 32 kDa protein. Using the same antibody, breakdown products of 16 kDa, 14 kDa and 12 kDa were observed. These products have also been observed in Zea mays and Solanum nigrum. Using DCMU during pulse-chase experiments at different light intensities, evidence was obtained that the 23.5 kDa breakdown product is generated in vivo.

  12. JCoDA: a tool for detecting evolutionary selection

    PubMed Central

    2010-01-01

    Background The incorporation of annotated sequence information from multiple related species in commonly used databases (Ensembl, Flybase, Saccharomyces Genome Database, Wormbase, etc.) has increased dramatically over the last few years. This influx of information has provided a considerable amount of raw material for evaluation of evolutionary relationships. To aid in the process, we have developed JCoDA (Java Codon Delimited Alignment) as a simple-to-use visualization tool for the detection of site specific and regional positive/negative evolutionary selection amongst homologous coding sequences. Results JCoDA accepts user-inputted unaligned or pre-aligned coding sequences, performs a codon-delimited alignment using ClustalW, and determines the dN/dS calculations using PAML (Phylogenetic Analysis Using Maximum Likelihood, yn00 and codeml) in order to identify regions and sites under evolutionary selection. The JCoDA package includes a graphical interface for Phylip (Phylogeny Inference Package) to generate phylogenetic trees, manages formatting of all required file types, and streamlines passage of information between underlying programs. The raw data are output to user configurable graphs with sliding window options for straightforward visualization of pairwise or gene family comparisons. Additionally, codon-delimited alignments are output in a variety of common formats and all dN/dS calculations can be output in comma-separated value (CSV) format for downstream analysis. To illustrate the types of analyses that are facilitated by JCoDA, we have taken advantage of the well studied sex determination pathway in nematodes as well as the extensive sequence information available to identify genes under positive selection, examples of regional positive selection, and differences in selection based on the role of genes in the sex determination pathway. Conclusions JCoDA is a configurable, open source, user-friendly visualization tool for performing evolutionary

  13. The Da Vinci code dynamically de-coded.

    PubMed

    Cohen, Mariam

    2005-01-01

    The novel The Da Vinci Code, by Dan Brown has been on best-seller lists for over two years. An examination of Brown's previous novels reveals a well-designed plot line shared by all four novels that not only makes them good "thrillers" but also creates a mythological structure to the novels that draws on common unconscious fantasies in the same way that fairy tales do. One aspect of this mythological structure is the use of evil conspiracies (and benign ones as well) for the protagonist to overcome. In addition, The Da Vinci Code presents a religious theme involving legends about Mary Magdalene. This theme touches on the role of a feminine aspect to divinity in allowing for an erotic connection with the divine. PMID:16448349

  14. [Lobectomy for lung cancer using the Da Vinci surgical system].

    PubMed

    Nakamura, Hiroshige

    2014-05-01

    Robot-assisted surgery using the da Vinci surgical system has attracted attention because of excellent operability without shaking by joint forceps under the clear vision of a three-dimensional high-definition camera in lung cancer surgery. Although this form of advanced medical care is not yet approved for insurance coverage, it is at the stage of clinical research and expected to be useful in hilar exposure, lymph node dissection, and suturing of the lung parenchyma or bronchus. Lung cancer surgery with the da Vinci system has the advantage of combining thoracotomy and minimally invasive surgery in video-assisted thoracic surgery. However, safety management, education, and significant cost are problems to be resolved. Several important issues such as sharing knowledge and technology of robotic surgery, education, training, development of new instruments, and acquisition of advanced medical insurance are discussed for the future development of robotic surgical systems. PMID:24946522

  15. DaVinci's Mona Lisa entering the next dimension.

    PubMed

    Carbon, Claus-Christian; Hesslinger, Vera M

    2013-01-01

    For several of Leonardo da Vinci's paintings, such as The Virgin and Child with St Anne or the Mona Lisa, there exist copies produced by his own studio. In case of the Mona Lisa, a quite exceptional, rediscovered studio copy was presented to the public in 2012 by the Prado Museum in Madrid. Not only does it mirror its famous counterpart superficially; it also features the very same corrections to the lower layers, which indicates that da Vinci and the 'copyist' must have elaborated their panels simultaneously. On the basis of subjective (thirty-two participants estimated painter-model constellations) as well as objective data (analysis of trajectories between landmarks of both paintings), we revealed that both versions differ slightly in perspective. We reconstructed the original studio setting and found evidence that the disparity between both paintings mimics human binocular disparity. This points to the possibility that the two Giocondas together might represent the first stereoscopic image in world history. PMID:24303752

  16. TA-DA: A TOOL FOR ASTROPHYSICAL DATA ANALYSIS

    SciTech Connect

    Da Rio, Nicola; Robberto, Massimo

    2012-12-01

    We present the Tool for Astrophysical Data Analysis (TA-DA), a new software aimed to greatly simplify and improve the analysis of stellar photometric data in comparison with theoretical models, and allow the derivation of stellar parameters from multi-band photometry. Its flexibility allows one to address a number of such problems: from the interpolation of stellar models, or sets of stellar physical parameters in general, to the computation of synthetic photometry in arbitrary filters or units; from the analysis of observed color-magnitude diagrams to a Bayesian derivation of stellar parameters (and extinction) based on multi-band data. TA-DA is available as a pre-compiled Interactive Data Language widget-based application; its graphical user interface makes it considerably user-friendly. In this paper, we describe the software and its functionalities.

  17. The da Vinci robot in right adrenalectomy: considerations on technique.

    PubMed

    D'Annibale, Annibale; Fiscon, Valentino; Trevisan, Paolo; Pozzobon, Maurizia; Gianfreda, Valeria; Sovernigo, Gianna; Morpurgo, Emilio; Orsini, Camillo; Del Monte, Daniele

    2004-02-01

    The da Vinci Robotic System (Intuitive Surgical, Mountain View, CA) became available at the General Surgery Department of Camposampiero Hospital in May 2001. From May 2001 to October 2002, 139 robotic operations were performed, one of which was a right adrenalectomy for a right adrenal mass. The progressive growth of the mass was the indication for surgical excision. Surgical adrenalectomy was successfully completed with da Vinci Robotic System using 5 ports (3 for the robotic system, 2 as service trocars). The wrist-like movements of the instrument's tip easily enabled the detachment of the right hepatic lobe from the gland and vessel isolation, while the 3-dimensional vision facilitated dissection of the veins from the vena cava. PMID:15259586

  18. PanDaTox: a tool for accelerated metabolic engineering

    SciTech Connect

    Amitai, Gil; Sorek, Rotem

    2012-04-18

    Metabolic engineering is often facilitated by cloning of genes encoding enzymes from various heterologous organisms into E. coli. Such engineering efforts are frequently hampered by foreign genes that are toxic to the E. coli host. We have developed PanDaTox (www.weizmann.ac.il/pandatox), a web-based resource that provides experimental toxicity information for more than 1.5 million genes from hundreds of different microbial genomes. The toxicity predictions, which were extensively experimentally verified, are based on serial cloning of genes into E. coli as part of the Sanger whole genome shotgun sequencing process. PanDaTox can accelerate metabolic engineering projects by allowing researchers to exclude toxic genes from the engineering plan and verify the clonability of selected genes before the actual metabolic engineering experiments are conducted.

  19. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... defined in 40 CFR 72.2, may use, as an alternative to the requirements specified in either paragraph (l... § 60.58b(i)(3) of subpart Eb of this part. (ii) Each 1-hour CO emissions average is calculated using... 40 Protection of Environment 6 2011-07-01 2011-07-01 false Emission monitoring. 60.49Da Section...

  20. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... affected unit that qualifies as a gas-fired or oil-fired unit, as defined in 40 CFR 72.2, may use, as an... § 60.58b(i)(3) of subpart Eb of this part. (ii) Each 1-hour CO emissions average is calculated using... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Emission monitoring. 60.49Da Section...

  1. Surgical treatment of parietal defects with "da Vinci" surgical robot.

    PubMed

    Vasilescu, D; Paun, S

    2012-06-12

    The robotic surgery has come through the development of telemedicine and minimally invasive surgery concepts, being developed in the military medicine by NASA during the years 1970-1980. The purpose of this paper is to briefly present our experience in the new field of the robotic surgery, by analyzing the results obtained over a lot of 20 patients operated with the "da Vinci" robot within the last 5 years in the Clinical Emergency Hospital Bucharest for various abdominal defects. PMID:22802899

  2. Improving Security in the ATLAS PanDA System

    NASA Astrophysics Data System (ADS)

    Caballero, J.; Maeno, T.; Nilsson, P.; Stewart, G.; Potekhin, M.; Wenaus, T.

    2011-12-01

    The security challenges faced by users of the grid are considerably different to those faced in previous environments. The adoption of pilot jobs systems by LHC experiments has mitigated many of the problems associated with the inhomogeneities found on the grid and has greatly improved job reliability; however, pilot jobs systems themselves must then address many security issues, including the execution of multiple users' code under a common 'grid' identity. In this paper we describe the improvements and evolution of the security model in the ATLAS PanDA (Production and Distributed Analysis) system. We describe the security in the PanDA server which is in place to ensure that only authorized members of the VO are allowed to submit work into the system and that jobs are properly audited and monitored. We discuss the security in place between the pilot code itself and the PanDA server, ensuring that only properly authenticated workload is delivered to the pilot for execution. When the code to be executed is from a 'normal' ATLAS user, as opposed to the production system or other privileged actor, then the pilot may use an EGEE developed identity switching tool called gLExec. This changes the grid proxy available to the job and also switches the UNIX user identity to protect the privileges of the pilot code proxy. We describe the problems in using this system and how they are overcome. Finally, we discuss security drills which have been run using PanDA and show how these improved our operational security procedures.

  3. Identification of an abundant 56 kDa protein implicated in food allergy as granule-bound starch synthase

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rice, the staple food of South and East Asian counties, is considered to be hypoallergenic. However, several clinical studies have documented rice-induced allergy in sensitive patients. Rice proteins with molecular weights of 14-16 kDa, 26 kDa, 33 kDa and 56 kDa have been identified as allergens. Re...

  4. Amelioration of Auditory Response by DA9801 in Diabetic Mouse

    PubMed Central

    Hong, Bin Na; Her, You Ri; Castañeda, Rodrigo; Moon, Hyo Won

    2015-01-01

    Diabetes mellitus (DM) is a metabolic disease that involves disorders such as diabetic retinopathy, diabetic neuropathy, and diabetic hearing loss. Recently, neurotrophin has become a treatment target that has shown to be an attractive alternative in recovering auditory function altered by DM. The aim of this study was to evaluate the effect of DA9801, a mixture of Dioscorea nipponica and Dioscorea japonica extracts, in the auditory function damage produced in a STZ-induced diabetic model and to provide evidence of the mechanisms involved in enhancing these protective effects. We found a potential application of DA9801 on hearing impairment in the STZ-induced diabetic model, demonstrated by reducing the deterioration produced by DM in ABR threshold in response to clicks and normalizing wave I–IV latencies and Pa latencies in AMLR. We also show evidence that these effects might be elicited by inducing NGF related through Nr3c1 and Akt. Therefore, this result suggests that the neuroprotective effects of DA9801 on the auditory damage produced by DM may be affected by NGF increase resulting from Nr3c1 via Akt transformation. PMID:25878713

  5. Amelioration of Auditory Response by DA9801 in Diabetic Mouse.

    PubMed

    Lee, Yeong Ro; Hong, Bin Na; Her, You Ri; Castañeda, Rodrigo; Moon, Hyo Won; Kang, Tong Ho

    2015-01-01

    Diabetes mellitus (DM) is a metabolic disease that involves disorders such as diabetic retinopathy, diabetic neuropathy, and diabetic hearing loss. Recently, neurotrophin has become a treatment target that has shown to be an attractive alternative in recovering auditory function altered by DM. The aim of this study was to evaluate the effect of DA9801, a mixture of Dioscorea nipponica and Dioscorea japonica extracts, in the auditory function damage produced in a STZ-induced diabetic model and to provide evidence of the mechanisms involved in enhancing these protective effects. We found a potential application of DA9801 on hearing impairment in the STZ-induced diabetic model, demonstrated by reducing the deterioration produced by DM in ABR threshold in response to clicks and normalizing wave I-IV latencies and Pa latencies in AMLR. We also show evidence that these effects might be elicited by inducing NGF related through Nr3c1 and Akt. Therefore, this result suggests that the neuroprotective effects of DA9801 on the auditory damage produced by DM may be affected by NGF increase resulting from Nr3c1 via Akt transformation. PMID:25878713

  6. OpenDA-NEMO framework for ocean data assimilation

    NASA Astrophysics Data System (ADS)

    van Velzen, Nils; Altaf, Muhammad Umer; Verlaan, Martin

    2016-05-01

    Data assimilation methods provide a means to handle the modeling errors and uncertainties in sophisticated ocean models. In this study, we have created an OpenDA-NEMO framework unlocking the data assimilation tools available in OpenDA for use with NEMO models. This includes data assimilation methods, automatic parallelization, and a recently implemented automatic localization algorithm that removes spurious correlations in the model based on uncertainties in the computed Kalman gain matrix. We have set up a twin experiment where we assimilate sea surface height (SSH) satellite measurements. From the experiments, we can conclude that the OpenDA-NEMO framework performs as expected and that the automatic localization significantly improves the performance of the data assimilation algorithm by successfully removing spurious correlations. Based on these results, it looks promising to extend the framework with new kinds of observations and work on improving the computational speed of the automatic localization technique such that it becomes feasible to include large number of observations.

  7. The ATLAS PanDA Pilot in Operation

    NASA Astrophysics Data System (ADS)

    Nilsson, P.; Caballero, J.; De, K.; Maeno, T.; Stradling, A.; Wenaus, T.; ATLAS Collaboration

    2011-12-01

    The Production and Distributed Analysis system (PanDA) [1-2] was designed to meet ATLAS [3] requirements for a data-driven workload management system capable of operating at LHC data processing scale. Submitted jobs are executed on worker nodes by pilot jobs sent to the grid sites by pilot factories. This paper provides an overview of the PanDA pilot [4] system and presents major features added in light of recent operational experience, including multi-job processing, advanced job recovery for jobs with output storage failures, gLExec [5-6] based identity switching from the generic pilot to the actual user, and other security measures. The PanDA system serves all ATLAS distributed processing and is the primary system for distributed analysis; it is currently used at over 100 sites worldwide. We analyze the performance of the pilot system in processing real LHC data on the OSG [7], EGI [8] and Nordugrid [9-10] infrastructures used by ATLAS, and describe plans for its evolution.

  8. Common commercial cosmetic products induce arthritis in the DA rat.

    PubMed Central

    Sverdrup, B; Klareskog, L; Kleinau, S

    1998-01-01

    Many different agents, including mineral oil and silicone, have the capacity to act as immunological adjuvants, i.e., they can contribute to the activation of the immune system. Some adjuvants, including mineral oil, are known to induce arthritis in certain strains of rats after intradermal injection or percutaneous application. The aim of this study was to determine if common commercial cosmetic products containing mineral oil could induce arthritis in the highly susceptible DA (Dark Agouti) rat. Intradermal injection of five out of eight assayed cosmetic products without further additives resulted in arthritis with synovitis. One of the products induced a very aggressive arthritis, which had declined after 5-9 weeks. When this product was also assayed for arthritogenicity upon percutaneous administration, it induced a mild and transient arthritis in 5 out of 10 DA rats, whereas control animals showed no clinical signs of joint involvement. No arthritic reaction was seen in rats after peroral feeding with the most arthritogenic product or by intravaginal application of Freund's adjuvants. Silicone gel implants in DA rats did not cause arthritis. We conclude that mineral oils included in common commercially available products retain their adjuvant properties and are arthritogenic in the presently investigated arthritis-prone rat strain. There is yet no evidence that mineral oils present in cosmetics may contribute to arthritis in humans, but we suggest that this question should be subject to further investigation. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 PMID:9417771

  9. OpenDA-NEMO framework for ocean data assimilation

    NASA Astrophysics Data System (ADS)

    van Velzen, Nils; Altaf, Muhammad Umer; Verlaan, Martin

    2016-03-01

    Data assimilation methods provide a means to handle the modeling errors and uncertainties in sophisticated ocean models. In this study, we have created an OpenDA-NEMO framework unlocking the data assimilation tools available in OpenDA for use with NEMO models. This includes data assimilation methods, automatic parallelization, and a recently implemented automatic localization algorithm that removes spurious correlations in the model based on uncertainties in the computed Kalman gain matrix. We have set up a twin experiment where we assimilate sea surface height (SSH) satellite measurements. From the experiments, we can conclude that the OpenDA-NEMO framework performs as expected and that the automatic localization significantly improves the performance of the data assimilation algorithm by successfully removing spurious correlations. Based on these results, it looks promising to extend the framework with new kinds of observations and work on improving the computational speed of the automatic localization technique such that it becomes feasible to include large number of observations.

  10. Basic principles of pharmaceutical science in Ayurvĕda.

    PubMed

    Subhose, Varanasi; Srinivas, Pitta; Narayana, Ala

    2005-01-01

    Pharmaceutical is one of the allied branches of science, which is closely associated with Medical science. Today pharmaceutical chemistry and pharmacognosy are playing important role in treatment for a disease and its prevention. Herbal medicines are being used by about 80% of the world population mostly in the developing countries in the primary health care. There has been an upsurge in demand for the Phyto-pharmaceutical products of Ayurvĕda in western nations, because of the fact that the synthetic drugs are considered to be unsafe. Due to this many national and multinational pharmaceutical companies are now concentrating on manufacturing of Ayurvĕdic Phyto-pharmaceutical products. Ayurvĕda is the Indian traditional system of medicine, which also deals about pharmaceutical science. The Ayurvĕdic knowledge of the pharmaceutical science is scattered in Ayurvĕdic classical texts. Săranghadhara Samhita, which is written by Săranghadhara, explain systematically about the information of the Ayurvĕdic pharmaceutical science and also updated it. Industrialized manufacturing of Ayurvĕdic dosage forms has brought in new challenges like deviation from basic concepts of medicine preparation. Săranghadhara Samrhită the devout text on pharmaceutics in Ayurvĕda comes handy to solve such problems, as the methods described are very lucid and easy to follow. PMID:17333665

  11. [The Arabic influence in the "Colóquios dos simples e drogas da India" of Garcia da Orta].

    PubMed

    Ricordel, Joëlle

    2015-09-01

    The "Colóquios dos simples e drogas he cousas medicinais de Índia" (Conversations on the simples, drugs and medicinal substances of India) (1563) of Garcia da Orta is a botanical and pharmacognosy book. The author is a Portuguese physician who studied in the Spanish universities and practiced medicine mainly in India. He studies in short chapters presented in the form of dialogues about sixty simples. Sources to which he refers are indicative of a "classical" training, but also the mark of a curious and open mind to different cultures. The Arabic sources are numerous and mainly concern the identification of substances by abundant synonyms of their names in foreign languages and different medicinal uses that may have been done by the ancient physicians. However, Da Orta is critical with respect to these sources, seeking contradictions and differences of opinion among authors. He confronts them with the oral information collected thanks to a wide network of contacts. PMID:26529894

  12. Seeking 'energy' vs. pain relief in spas in Brazil (Caldas da Imperatriz) and Portugal (Termas da Sulfurea).

    PubMed

    Quintela, Maria Manuel

    2011-04-01

    This paper is a comparative ethnography of the therapeutic practices at two different spa locations: Caldas da Imperatriz, SC, Brazil, and Termas da Sulfurea in Cabeco de Vide, Portugal. The comparison reveals the existence of contrasting 'explanatory models' held by the spa-goers as well as by the official medical systems. In the Portuguese context this model is highly medicalized; in the Brazilian case, spa treatments are viewed as 'alternative' or 'complementary' therapy and are also related to religious philosophies. Each model corresponds to a different idiom expressing certain experiences and world views, one focusing on 'pains' (dores) and the other on 'energy' (energia), the former leading to the rationale of 'curing', the latter to the notion of 'energizing'. In this paper the author intends to analyze and contrast the categories found in these models, which originate from different conceptions of health, illness and healing for Brazilian and Portuguese spa-goers. PMID:21563001

  13. The 29-kDa proteins phosphorylated ion thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein

    SciTech Connect

    Mendelsohn, M.E.; Yan Zhu; O'Neill, S. )

    1991-12-15

    Thrombin plays a critical role in platelet activation, hemostasis, and thrombosis. Cellular activation by thrombin leads to the phosphorylation of multiple proteins, most of which are unidentified. The authors have characterized several 29-kDa proteins that are rapidly phosphorylated following exposure of intact human platelets to thrombin. A murine monoclonal antibody raised to an unidentified estrogen receptor-related 29-kDa protein selectively recognized these proteins as well as a more basic, unphosphorylated 27-kDa protein. Cellular activation by thrombin led to a marked shift in the proportion of protein from the 27-kDa unphosphorylated form to the 29-kDa phosphoprotein species. Using this antibody, they isolated and sequenced a human cDNA clone encoding a protein that was identical to the mammalian 27-kDa heat shock protein (HSP27), a protein of uncertain function that is known to be phosphorylated to several forms and to be transcriptionally induced by estrogen. The 29-kDa proteins were confirmed to be phosphorylated forms of HSP27 by immunoprecipitation studies. Thus, the estrogen receptor-related protein is HSP27, and the three major 20-kDa proteins phosphorylated in thrombin-activated platelets are forms of HSP27. These data suggest a role for HSP27 in the signal transduction events of platelet activation.

  14. Identification of sequence similarity between 60 kDa and 70 kDa molecular chaperones: evidence for a common evolutionary background?

    PubMed Central

    Flores, A I; Cuezva, J M

    1997-01-01

    Recent findings support the premise that chaperonins (60 kDa stress-proteins) and alpha-subunits of F-type ATPases (alpha-ATPase) are evolutionary related protein families. Two-dimensional gel patterns of synthesized proteins in unstressed and heat-shocked embryonic Drosophila melanogaster SL2 cells revealed that antibodies raised against the alpha-subunit of the F1-ATPase complex from rat liver recognize an inducible p71 member of the 70 kDa stress-responsive protein family. Molecular recognition of this stress-responsive 70 kDa protein by antibodies raised against the F1-ATPase alpha-subunit suggests the possibility of partial sequence similarity within these ATP-binding protein families. A multiple sequence alignment between alpha-ATPases and 60 kDa and 70 kDa molecular chaperones is presented. Statistical evaluation of sequence similarity reveals a significant degree of sequence conservation within the three protein families. The finding suggests a common evolutionary origin for the ATPases and molecular chaperone protein families of 60 kDa and 70 kDa, despite the lack of obvious structural resemblance between them. PMID:9065788

  15. Stereoscopic augmented reality for da Vincii™ robotic biliary surgery

    PubMed Central

    Volonté, Francesco; Buchs, Nicolas C.; Pugin, François; Spaltenstein, Joël; Jung, Minoa; Ratib, Osman; Morel, Philippe

    2013-01-01

    INTRODUCTION New laparoscopic techniques put distance between the surgeon and his patient. PRESENTATION OF CASE 3D volume rendered images directly displayed in the da Vinci surgeon's console fill this gap by allowing the surgeon to fully immerse in its intervention. DISCUSSION During the robotic operation the surgeon has a greater control on the procedure because he can stay more focused not being obliged to turn is sight out of his operative field. Moreover, thanks to depth perception of the rendered images he had a precise view of important anatomical structures. CONCLUSION We describe our preliminary experience in the quest of computer-assisted robotic surgery. PMID:23466685

  16. Ultratight crystal packing of a 10 kDa protein

    SciTech Connect

    Trillo-Muyo, Sergio; Chruszcz, Maksymilian; Minor, Wladek; Kuisiene, Nomeda

    2013-03-01

    The crystal structure of the C-terminal domain of a putative U32 peptidase from G. thermoleovorans is reported; it is one of the most tightly packed protein structures reported to date. While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air-sensitive high-solvent-content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C-terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.

  17. A quasi-passive CMOS pipeline D/A converter

    NASA Technical Reports Server (NTRS)

    Wang, Fong-Jim; Temes, Gabor C.; Law, Simon

    1989-01-01

    A novel pipeline digital-to-analog converter configuration, based on switched-capacitor techniques, is described. An n-bit D/A conversion can be implemented by cascading n + 1 unit cells. The device count of the circuit increases linearly, not exponentially, with the conversion accuracy. The new configuration can be pipelined. Hence, the conversion rate can be increased without requiring a higher clock rate. An experimental 10-bit DAC prototype has been fabricated using a 3-micron CMOS process. The results show that high-speed, high-accuracy, and low-power operation can be achieved without special process or postprocess trimming.

  18. Leonardo da Vinci and the origin of semen

    PubMed Central

    Noble, Denis; DiFrancesco, Dario; Zancani, Diego

    2014-01-01

    It is well known that Leonardo da Vinci made several drawings of the human male anatomy. The early drawings (before 1500) were incorrect in identifying the origin of semen, where he followed accepted teaching of his time. It is widely thought that he did not correct this mistake, a view that is reflected in several biographies. In fact, he made a later drawing (after 1500) in which the description of the anatomy is remarkably accurate and must have been based on careful dissection. In addition to highlighting this fact, acknowledged previously in only one other source, this article reviews the background to Leonardo's knowledge of the relevant anatomy. PMID:27494016

  19. Visual tracking of da Vinci instruments for laparoscopic surgery

    NASA Astrophysics Data System (ADS)

    Speidel, S.; Kuhn, E.; Bodenstedt, S.; Röhl, S.; Kenngott, H.; Müller-Stich, B.; Dillmann, R.

    2014-03-01

    Intraoperative tracking of laparoscopic instruments is a prerequisite to realize further assistance functions. Since endoscopic images are always available, this sensor input can be used to localize the instruments without special devices or robot kinematics. In this paper, we present an image-based markerless 3D tracking of different da Vinci instruments in near real-time without an explicit model. The method is based on different visual cues to segment the instrument tip, calculates a tip point and uses a multiple object particle filter for tracking. The accuracy and robustness is evaluated with in vivo data.

  20. Da Vinci's codex and the anatomy of healthcare.

    PubMed

    Stephens-Borg, Keith

    2012-08-01

    We usually display a laid-back approach to medical jargon throughout our theatre work. The word 'perioperative' is built from the Greek word 'peri' (around) and the Latin 'operari' (to work). Latin and Greek became the prefixed language of choice for Leonardo da Vinci, and his research was pivotal in determining the way in which surgical procedures are documented. Ancient manuscripts aided the unfolding of the secrets of anatomy, and Leonardo revealed that art was the key in expressive detailed explanation. PMID:23248927

  1. Evolution of the ATLAS PanDA workload management system for exascale computational science

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Klimentov, A.; Nilsson, P.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.; Yu, D.; Atlas Collaboration

    2014-06-01

    An important foundation underlying the impressive success of data processing and analysis in the ATLAS experiment [1] at the LHC [2] is the Production and Distributed Analysis (PanDA) workload management system [3]. PanDA was designed specifically for ATLAS and proved to be highly successful in meeting all the distributed computing needs of the experiment. However, the core design of PanDA is not experiment specific. The PanDA workload management system is capable of meeting the needs of other data intensive scientific applications. Alpha-Magnetic Spectrometer [4], an astro-particle experiment on the International Space Station, and the Compact Muon Solenoid [5], an LHC experiment, have successfully evaluated PanDA and are pursuing its adoption. In this paper, a description of the new program of work to develop a generic version of PanDA will be given, as well as the progress in extending PanDA's capabilities to support supercomputers and clouds and to leverage intelligent networking. PanDA has demonstrated at a very large scale the value of automated dynamic brokering of diverse workloads across distributed computing resources. The next generation of PanDA will allow other data-intensive sciences and a wider exascale community employing a variety of computing platforms to benefit from ATLAS' experience and proven tools.

  2. Effects of DA-Phen, a dopamine-aminoacidic conjugate, on alcohol intake and forced abstinence.

    PubMed

    Sutera, Flavia Maria; De Caro, Viviana; Cannizzaro, Carla; Giannola, Libero Italo; Lavanco, Gianluca; Plescia, Fulvio

    2016-09-01

    The mesolimbic dopamine (DA) system plays a key role in drug reinforcement and is involved in the development of alcohol addiction. Manipulation of the DAergic system represents a promising strategy to control drug-seeking behavior. Previous studies on 2-amino-N-[2-(3,4-dihydroxy-phenyl)-ethyl]-3-phenyl-propionamide (DA-Phen) showed in vivo effects as a DA-ergic modulator. This study was aimed at investigate DA-Phen effects on operant behavior for alcohol seeking behavior, during reinstatement following subsequent periods of alcohol deprivation. For this purpose, male Wistar rats were tested in an operant paradigm of self-administration; behavioral reactivity and anxiety like-behavior during acute abstinence were evaluated. A characterization of DA-Phen CNS targeting by its quantification in the brain was also carried out. Our findings showed that DA-Phen administration was able to reduce relapse in alcohol drinking by 50% and reversed the alterations in behavioral reactivity and emotionality observed during acute abstinence. In conclusion, DA-Phen can reduce reinstatement of alcohol drinking in an operant-drinking paradigm following deprivation periods and reverse abstinence-induced behavioral phenotype. DA-Phen activity seems to be mediated by the modulation of the DAergic transmission. However further studies are needed to characterize DA-Phen pharmacodynamic and pharmacokinetic properties, and its potential therapeutic profile in alcohol addiction. PMID:27155501

  3. [Textual Research on Chiding Disease and Matixiang Herb in Da he wai ke (External Medicine of Da He)].

    PubMed

    Pu, Xiao-Lan; Zhang, Yi; Yang, Wen-Yu; Wu, Ya-Mei

    2012-11-01

    Recorded in Da he wai ke (The External Medicine of Da He) in the Ming dynasty without a corresponding disease title in contemporary scholarly concepts of Traditional Chinese Medicine (TCM), Chiding disease was described as an acute symptomatic disease with inevitable serious conditions of convulsion-like or mania-like behavior, and as a refractory ailment. It was also considered as an alias of Hongsiding (namely the acute lymphangitis) in few ancient books. Matixiang, a Chinese herb for treatment of Chiding, was regarded as a nonsuch drug by Da he wai ke, and was considered as a take-then-cure drug by Chuang yang jing yan quan shu (Compendium for Experience in Sores Diseases). The present studies about Chiding and Matixiang show that: (1) the name of Chiding was first seen in Zhong zang jing (Master Hua's Classic of the Viscera) ; (2) Chiding was rarely mentioned in medical literature of the Song, Yuan and Qing Dynasty, but mostly seen in the Ming Dynasty; (3) Xinding and Huoyanding were found as synonyms of Chiding ; (4) the distinction between Chiding and Hongsiding (flaming sore) was revealed by comparison analysis of their etiology, affected part(s), symptoms, prognosis and syndromic pictures; (5) Chiding on the tongue is the same as "Tongue Ding" or "Sublingual Ding" in contemporary TCM and as "Sublingual Space Infection" in Western Medicine. Moreover, Chiding on the hands, feet or other skin parts could be classified as "Hands/Feet Ding" in contemporary TCM and as "Furuncle" (i.e., Acute Suppurative Folliculitis and Perifolliculitis or cellulites of the tongue) in Western Medicine; (6) Matixiang is probably deduced as Asarum forbesii Maxim., A. ichangense C. Y. Cheng et C. S. Yang, family Aristolochiaceae, or Valeriana jatamansi Jones., family Valerianaceae. PMID:23363849

  4. Chandra Confirmation of a Pulsar Wind Nebula in DA 495

    NASA Technical Reports Server (NTRS)

    Arzoumanian, Z.; Safi-Harb, S.; Landecker, T.L.; Kothes, R.; Camilo, F.

    2008-01-01

    As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected X-ray source at the heart of the annular radio nebula, establishing the radiative properties of two key emission components: a soft unresolved source with a blackbody temperature of 1 MK consistent with a neutron star, surrounded by a nontherma1 nebula 40" in diameter exhibiting a power-law spectrum with photon index Gamma = 1.63, typical of a pulsar wind nebula. Morphologically, the nebula appears to be slightly extended along a direction, in projection on the sky, previously demonstrated to be of significance in radio and ASCA observations; we argue that this represents the orientation of the pulsar spin axis. At smaller scales, a narrow X-ray feature is seen extending out 5" from the point source, but energetic arguments suggest that it is not the resolved termination shock of the pulsar wind against the ambient medium. Finally, we argue based on synchrotron lifetimes in the nebular magnetic field that DA 495 represents the first example of a pulsar wind nebula in which electromagnetic flux makes up a significant part, together with particle flux, of the neutron star's wind.

  5. The DA+dMe Binary WD 0334-6400

    NASA Astrophysics Data System (ADS)

    Barrett, P. E.; Schlegel, E. M.; Dupuis, J.; Heathcote, B.

    2005-12-01

    Multiwavelength observations of the star WD 0334-6400 (or Ret 1 in A Catalog and Altas of Cataclysmic Variables: Living Edition) show it to be a DA+dMe binary. Our analysis of the far-ultraviolet continuum and spectral lines reveal that Teff = 36500 K; log g = 7.7; and the photosphere contains C, N, O, Si, P, and S. C/H = 2 x 10-6, which is the highest abundance seen in a DA white dwarf at this temperature. The dMe star is seen as a flux excess in near-infrared photometry and appears to show occasional flaring of about one magnitude as seen in the Harvard plates. There is no evidence of periodic variability in the spectroscopic or photometric data. This paper is based on observations made with the NASA-CNES-CSA FUSE. This research is partially supported by NASA FUSE grants NAG5-12413 and NNG04GL18G.

  6. The 37kDa/67kDa Laminin Receptor acts as a receptor for Aβ42 internalization

    PubMed Central

    Da Costa Dias, Bianca; Jovanovic, Katarina; Gonsalves, Danielle; Moodley, Kiashanee; Reusch, Uwe; Knackmuss, Stefan; Weinberg, Marc S.; Little, Melvyn; Weiss, Stefan F. T.

    2014-01-01

    Neuronal loss is a major neuropathological hallmark of Alzheimer's disease (AD). The associations between soluble Aβ oligomers and cellular components cause this neurotoxicity. The 37 kDa/67 kDa laminin receptor (LRP/LR) has recently been implicated in Aβ pathogenesis. In this study the mechanism underlying the pathological role of LRP/LR was elucidated. Försters Resonance Energy Transfer (FRET) revealed that LRP/LR and Aβ form a biologically relevant interaction. The ability of LRP/LR to form stable associations with endogenously shed Aβ was confirmed by pull down assays and Aβ-ELISAs. Antibody blockade of this association significantly lowered Aβ42 induced apoptosis. Furthermore, antibody blockade and shRNA mediated downregulation of LRP/LR significantly hampered Aβ42 internalization. These results suggest that LRP/LR is a receptor for Aβ42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide by facilitating intra-cellular Aβ42 accumulation. These findings recommend anti-LRP/LR specific antibodies and shRNAs as potential therapeutic tools for AD treatment. PMID:24990253

  7. The Indicative and Subjunctive "da"-complements in Serbian A Syntactic-Semantic Approach

    ERIC Educational Resources Information Center

    Todorovic, Natasa

    2012-01-01

    A syntactic-semantic investigation of subjunctive and indicative "da"-complements in Serbian is conducted in this project. After a careful comparison of Serbian sentence constructions with "da"-complements to the equivalent sentence structures in languages of the Balkans as well as other Slavic languages, it is clearly…

  8. Paśu Ayurvĕda (veterinary medicine) in Garudapurăņa.

    PubMed

    Varanasi, Subhose; Narayana, A

    2007-01-01

    The history of veterinary medicine is closely tied to the development of human medicine. Evidence of animal medicine has been found in ancient civilizations, such as those of the Hindu, Babylonians, Hebrews, Arabs, Greeks, and Romans. Ancient Indian literature in the form of the holy Vĕda, Purăna, Brăhmaņa, epics, etc. is flooded with information on animal care. The Purăņa are ancient scriptures discuss varied topics like devotion to God and his various aspects, traditional sciences like Ayurvĕda, Jyŏtişa (Astrology), cosmology, concepts like dharma, karma, reincarnation and many others. The treatment of animal diseases using Ayurvedic medicine has been mentioned in Garudapurăna, Agnipurăņa, Atri-samhită, Matsyapurăņa and many other texts. The Garudapurăņa is one of the important Săttvika purăna, the subject matter is divided into two parts, viz. Pŭrvakhaņda (first part) and an Uttarakhaņda (subsequent part). Gavăyurvĕda, Gajăyurvĕda narrated briefly and Aśvăyurvĕda described detailly in Pŭrvakhaņda. PMID:19580108

  9. A Value-Engaged Approach for Evaluating the Bunche-Da Vinci Learning Academy

    ERIC Educational Resources Information Center

    Greene, Jennifer C.

    2005-01-01

    In 2001, the Bunche Academy was chosen by its district to join in partnership with the Da Vinci Learning Corporation to embark on an ambitious whole-school reform initiative, especially designed by the corporation for low-performing schools. In this chapter, the author describes how, as illustrated in the Bunche-Da Vinci Learning Academy context,…

  10. Processing and Visualizing Planetary Data Using DaVinci: Updates for Portability and Scriptable Execution

    NASA Astrophysics Data System (ADS)

    Edwards, C. S.; Anwar, S.; Hagee, W.; Doerres, D.; Dickensheid, S.; Christensen, P. R.

    2015-06-01

    We present updates to the DaVinci software toolkit that enable stand-alone reading of ISIS3 files, a link between DaVinci and JMARS, the ingestion and use of geospatial information, and a series of executable THEMIS processing scripts.

  11. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form...

  12. Topological studies of spinach 22 kDa protein of Photosystem II.

    PubMed

    Kim, S; Pichersky, E; Yocum, C F

    1994-12-30

    An intrinsic 22 kDa polypeptide is associated with the O2-evolving Photosystem II core complex in a variety of green plants, although it does not appear to be required for O2 evolution. Digestion of thylakoid membranes and isolated Photosystem II preparations with trypsin, followed by immunoblotting using spinach anti-22 kDa antibodies, leads to two observations: (1) the domain between the 2nd and 3rd transmembrane helices of the 22 kDa protein is stromally exposed, and (2) only in a reaction center complex preparation, lacking the chlorophyll a/b-light harvesting complex II, is there extensive proteolytic cleavage of the 22 kDa protein. We also found that after, but not prior to, selective extraction of the 22 and 10 kDa proteins from Photosystem II membranes, the chlorophyll a/b-light harvesting complex II can be separated from the Photosystem II reaction center core by precipitation with MgCl2. This result suggests that the 22 kDa polypeptide is located between the Photosystem II reaction center polypeptides and light-harvesting complex II; it is possible that the protein serves as a link between the two protein complexes. The presence of the 22 kDa protein in several species was also examined by immunoblotting with polyclonal spinach anti-22 kDa antibodies. PMID:7803450

  13. 32 CFR Appendix E to Part 623 - Surety Bond (DA Form 4881-3-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Surety Bond (DA Form 4881-3-R) E Appendix E to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. E Appendix E to Part 623—Surety Bond (DA Form...

  14. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form...

  15. A Creative Approach to the Common Core Standards: The Da Vinci Curriculum

    ERIC Educational Resources Information Center

    Chaucer, Harry

    2012-01-01

    "A Creative Approach to the Common Core Standards: The Da Vinci Curriculum" challenges educators to design programs that boldly embrace the Common Core State Standards by imaginatively drawing from the genius of great men and women such as Leonardo da Vinci. A central figure in the High Renaissance, Leonardo made extraordinary contributions as a…

  16. The Effect of DA-9701 in Opioid-induced Bowel Dysfunction of Guinea Pig

    PubMed Central

    Hussain, Zahid; Rhee, Kwang Won; Lee, Young Ju; Park, Hyojin

    2016-01-01

    Background/Aims Opioid induced bowel dysfunction (OIBD) is associated with decreased gastrointestinal (GI) propulsive activity due to intake of opioid analgesics. DA-9701, a novel prokinetic agent formulated with Pharbitis Semen and Corydalis Tuber has promising effects on GI motor function. Therefore, we aim to evaluate the prokinetic effects of DA-9701 in an OIBD model of guinea pig. Methods The ileal and distal colon muscle contraction in presence of different doses of DA-9701, morphine, and combination (morphine + DA-9701) was measured by tissue bath study. The prokinetic effect of DA-9701 was assessed by charcoal transit and fecal pellet output assay in an OIBD model of guinea pig. Results DA-9701 significantly increased the amplitude and area under the curve of ileal muscle contraction, while there was insignificant effect on the distal colon compared to the control. The maximal amplitude of ileal muscle contraction was acquired at a concentration of 10 μg/mL of DA-9701. In contrast, morphine significantly decreased the amplitude of ileal and distal colon muscle contraction compared to the control. Morphine delayed both upper (P < 0.01) and lower (P < 0.05) GI transit, and delayed GI transit was restored by the administration of DA-9701. Morphine induced reduction of contractility was significantly ameliorated by addition of DA-9701 in both ileal and distal colon muscles. Conclusions DA-9701 significantly increased the amplitude of contraction of the ileal muscle, however the distal colon muscle contraction was insignificant. Additionally, it restored delayed upper and lower GI transit in an OIBD model of guinea pig, and it might prove to be a useful candidate drug in a clinical trial for OIBD. PMID:26932898

  17. Pharmacological action of DA-9701 on the motility of feline stomach circular smooth muscle.

    PubMed

    Nguyen, Thanh Thao; Song, Hyun Ju; Ko, Sung Kwon; Sohn, Uy Dong

    2015-03-01

    DA-9701, a new prokinetic agent for the treatment of functional dyspepsia, is formulated with Pharbitis semen and Corydalis tuber. This study wasconducted to determine the pharmacological action of DA-9701 and to identify the receptors involved in DA-9701 -induced contractile responsesin the feline gastric corporal, fundic and antral circular smooth muscle. Concentration-response curve to DA-9701 was established. The tissue trips were exposed to methylsergide, ketanserin, ondansetron, GR 113808, atropine and dopamine before administration of DA-9701. The contractile force was determined before and after administration of drugs by a polygraph.DA-9701 enhanced the spontaneous contractile amplitude of antrum, corpus and fundus. However, it did not change the spontaneous contractile frequency of antrum and corpus, but concentration-dependently reduced that of fundus. In the fundus, DA-9701 -induced tonic contractions were inhibited by dopamine, methylsergide, ketanserine, ondansetron or GR 113808 respectively, but not by atropine, indicating that the contractile responses are mediated by multiple receptors: 5-HT2, 5-HT3, 5-HT4, and dopamine receptors. In the corpus, DA-9701-induced contractions were blocked by atropine, dopamine or GR 113808, but not by methysergide, ketanserin or ondansetron, indicating that they are involved in receptors on both, smooth muscles and neurons: 5-HT4 and dopamine receptors. However, contractile responses to DA-9701 are mainly mediated by dopamine receptors in the antrum. These results suggest that DA-9701 has important roles in gastric accommodation by enhancing tonic activity of fundus, and in gastric emptying and gastrointestinal transit by phasic contractions of corpus and antrum mediated by multiple receptors. PMID:25980179

  18. A 92-kDa human immunostimulatory protein.

    PubMed Central

    Fontan, E; Briend, E; Saklani-Jusforgues, H; d'Alayer, J; Vandekerckhove, J; Fauve, R M

    1994-01-01

    We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 micrograms/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety. Images PMID:8078887

  19. DBS -- An rlogin multiplexer and output logger for DA systems

    SciTech Connect

    Oleynik, G.; Appleton, L.; Udumula, L.; Votava, M.

    1994-04-01

    DART Bootstrap Services (dbs) is the first component of run-control for the DART Data Acquisition system -- the DA for the 96` round of experiments at Fermilab -- though it has potential usefulness as a powerful tool in other distributed applications. dbs is an rlogin session multiplexer. It allows a user, running a single program, to start up any number of remote login sessions, feed shell commands to them, and collect their output into a single (or multiple) log files (a server keeps the sessions open and collects their output). From this program, any session can be attached to interactively so it appears just like an rlogin session -- dbs becomes transparent. When finished with this interactive mode, the user can escape back to dbs and attach to a different session if so desired. Among many other useful features, dbs supplies a mechanism for cleanup (deletion) of all processes created under a session, allowing a fresh start.

  20. VLBI Radar of the 2012 DA14 Asteroid

    NASA Astrophysics Data System (ADS)

    Nechaeva, M. B.; Dugin, N. A.; Antipenko, A. A.; Bezrukov, D. A.; Bezrukov, V. V.; Voytyuk, V. V.; Dement'ev, A. F.; Jekabsons, N.; Klapers, M.; Konovalenko, A. A.; Kulishenko, V. F.; Nabatov, A. S.; Nesteruk, V. N.; Putillo, D.; Reznichenko, A. M.; Salerno, E.; Snegirev, S. D.; Tikhomirov, Yu. V.; Khutornoy, R. V.; Skirmante, K.; Shmeld, I.; Chagunin, A. K.

    2015-03-01

    An experiment on VLBI radar of the 2012 DA14 asteroid was carried out on February 15-16, 2011 at the time of its closest approach to the Earth. The research teams of Kharkov (Institute of Radio Astronomy of the National Academy of Sciences of Ukraine), Evpatoria (National Space Facilities Control and Test Center), Nizhny Novgorod (Radiophysical Research Institute), Bologna (Istituto di Radioastronomia (INAF)), and Ventspils (Ventspils International Radioastronomy Center) took part in the experiment. The asteroid was irradiated by the RT-70 planetary radar (Evpatoria) at a frequency of 5 GHz. The reflected signal was received using two 32-m radio telescopes in Medicina (Italy) and Irbene (Latvia) in radiointerferometric mode. The Doppler frequency shifts in bi-static radar mode and interference frequency in VLBI mode were measured. Accuracy of the VLBI radar method for determining the radial and angular velocities of the asteroid were estimated.

  1. 70 DA WHITE DWARFS IDENTIFIED IN LAMOST PILOT SURVEY

    SciTech Connect

    Zhao, J. K.; Luo, A. L.; Zhao, G.; Oswalt, T. D. E-mail: gzhao@bao.ac.cn E-mail: toswalt@fit.edu

    2013-06-01

    We present a spectroscopically identified catalog of 70 DA white dwarfs (WDs) from the LAMOST pilot survey. Thirty-five are found to be new identifications after cross-correlation with the Eisenstein et al. and Villanova catalogs. The effective temperature and gravity of these WDs are estimated by Balmer lines fitting. Most of them are hot WDs. The cooling times and masses of these WDs are estimated by interpolation in theoretical evolution tracks. The peak of the mass distribution is found to be {approx}0.6 M {sub Sun }, which is consistent with prior work in the literature. The distances of these WDs are estimated using the method of synthetic spectral distances. All of these WDs are found to be in the Galactic disk from our analysis of space motions. Our sample supports the expectation that WDs with high mass are concentrated near the plane of the Galactic disk.

  2. DBS - an rlogin multiplexor and output logger for DA systems

    SciTech Connect

    Oleynik, G.; Appleton, L.; Udumula, L.; Votava, M.

    1994-12-31

    DART Bootstrap Services (dbs) is the first component of run-control for the DART Data Acquisition system - the DA for the 96{prime} round of experiments at Fermilab - though it has potential usefulness as a powerful tool in other distributed applications, dbs is an rlogin session multiplexer. It allows a user, running a single program, to start up any number of remote login sessions, feed shell commands to them, and collect their output into a single (or multiple) logfiles (a server keeps the sessions open and collects their output). From this program, any session can be attached to interactively so it appears just like an rlogin session - dbs becomes transparent. When finished with this interactive mode, the user can escape back to dbs and attach to a different session if so desired. Among many other useful features, dbs supplies a mechanism for cleanup (deletion) of all processes created under a session, allowing a fresh start.

  3. [Regarding the Manuscript D " Dell' occhio " of Leonardo da Vinci].

    PubMed

    Heitz, Robert F

    2009-01-01

    Leonardo da Vinci's Manuscript D consists of five double pages sheets, which, folded in two, comprise ten folios. This document, in the old Tuscan dialect and mirror writing, reveals the ideas of Leonardo on the anatomy of the eye in relation to the formation of images and visual perception. Leonardo explains in particular the behavior of the rays in the eye in terms of refraction and reflection, and is very mechanistic in his conception of the eye and of the visual process. The most significant innovations found in these folios are the concept of the eye as a camera obscura and the intersection of light rays in the interior of the eye. His texts nevertheless show hesitation, doubts and a troubled confusion, reflecting the ideas and uncertainties of his era. He did not share his results in his lifetime, despite both printing and etching being readily available to him. PMID:19852385

  4. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... SOURCES Standards of Performance for Electric Utility Steam Generating Units § 60.45Da Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  5. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... SOURCES Standards of Performance for Electric Utility Steam Generating Units § 60.45Da Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  6. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... SOURCES Standards of Performance for Electric Utility Steam Generating Units § 60.45Da Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  7. Antimicrobial Properties of an Immunomodulator - 15 kDa Human Granulysin

    PubMed Central

    Wei, Hung-Mu; Lin, Li-Chih; Wang, Chiu-Feng; Lee, Yi-Jang; Chen, Yuan-Tsong; Liao, You-Di

    2016-01-01

    Granulysin, a cationic protein expressed by human natural killer cells and cytotoxic T lymphocytes, is a mediator for drug-induced Stevens-Johnson syndrome and graft-versus-host disease. Some 15 kDa granulysin are processed into 9 kDa forms and sequestered in cytolytic granules, while others are constitutively secreted into body fluids. Both 9 and 15 kDa granulysin have been shown to be a serum marker for cell-mediated immunity. Furthermore, 15 kDa is able to activate monocyte differentiation. However, its antimicrobial properties have not been clearly addressed. Here, we report a novel method to prepare both the soluble 9 and 15 kDa granulysin and show that the 15 kDa form is more effective than the 9 kDa form in exerting specific antimicrobial activity against Pseudomonas aeruginosa within a range of few micromolars. We also show that the 15 kDa granulysin is able to hyperpolarize the membrane potential and increase membrane permeability of treated bacteria. Interestingly, the bactericidal activity and membrane permeability of the granulysins were markedly reduced at lower pH (pH 5.4) as a result of probable increase in hydrophobicity of the granulysins. Additionally, we’ve also shown the granulysin to inhibit biofilm formation by P. aeruginosa. These results suggest that the 15 kDa granulysin exhibits a novel mechanism in bacteria killing in a way that’s different from most antimicrobial peptides. Our novel granulysin preparation methodology will be useful for further study of action mechanisms of other antimicrobial, cytotoxic and immunomodulating properties in granulysin-mediated diseases. PMID:27276051

  8. Neospora caninum: identification of 19-, 38-, and 40-kDa surface antigens and a 33-kDa dense granule antigen using monoclonal antibodies.

    PubMed

    Schares, G; Dubremetz, J F; Dubey, J P; Bärwald, A; Loyens, A; Conraths, F J

    1999-06-01

    Neospora caninum, a coccidian parasite closely related to Toxoplasma gondii, can infect a broad host range and is regarded as an important cause of bovine abortion worldwide. In the present study, four antigens of N. caninum were partially characterized using monoclonal antibodies. Immunofluorescence of viable tachyzoites as well as the immunoprecipitation of antigens extracted from tachyzoites previously labeled by surface biotinylation revealed that three of these antigens with apparent molecular weights of 40, 38, and 19 kDa are located in the outer surface membrane of this parasite stage. Further evidence for the surface localization of the 38-kDa antigen was obtained by immunoelectron microscopy. In addition to the surface molecules, an antigen located in dense granules and in the tubular network of the parasitophorous vacuole was detected by another monoclonal antibody. When tachyzoite antigens separated under nonreducing conditions were probed on Western blots, this antibody reacted mainly with a 33-kDa antigen. Immunohistochemical analysis of infected tissue sections indicated that the 33-kDa dense granule antigen is present in both tachyzoites and bradyzoites, while the 38-kDa surface antigen from tachyzoites seems to be absent in bradyzoites. PMID:10366536

  9. Respostas religiosas à aids no Brasil: impressões de pesquisa acerca da Pastoral de DST/Aids da Igreja Católica1

    PubMed Central

    Seffner, Fernando; Silva, Cristiane Gonçalves Meireles da; Maksud, Ívia; Garcia, Jonathan; Rios, Luís Felipe; Natividade, Marcelo; Borges, Priscila Rodrigues; Parker, Richard; Terto, Veriano

    2009-01-01

    O texto encontra-se estruturado em quatro partes. Na primeira delas, apresentamos um conjunto de considerações e informações acerca da situação da aids no Brasil, das relações entre religião, sexualidade, aids e estado laico, bem como uma descrição mais clara do Projeto Respostas Religiosas ao HIV/Aids no Brasil, do qual este texto apresenta algumas impressões de pesquisa preliminares. A seguir, dedicamos um item a apresentação da Pastoral de DST/Aids, sua história, estrutura e objetivos. No item seguinte problematizamos diversas questões em particular no âmbito das relações Estado e Igreja, relações entre agentes de pastoral e hierarquia da Igreja, e questões ligadas mais diretamente à sexualidade e aids, todas referenciadas ao trabalho da Pastoral de DST/Aids. Ao final, apresentamos a bibliografia e fontes consultadas. PMID:20442806

  10. PD2P: PanDA Dynamic Data Placement for ATLAS

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Panitkin, S.

    2012-12-01

    The PanDA (Production and Distributed Analysis) system plays a key role in the ATLAS distributed computing infrastructure. PanDA is the ATLAS workload management system for processing all Monte-Carlo (MC) simulation and data reprocessing jobs in addition to user and group analysis jobs. The PanDA Dynamic Data Placement (PD2P) system has been developed to cope with difficulties of data placement for ATLAS. We will describe the design of the new system, its performance during the past year of data taking, dramatic improvements it has brought about in the efficient use of storage and processing resources, and plans for the future.

  11. Stability of new carbapenem DA-1131 to renal dipeptidase (dehydropeptidase I).

    PubMed

    Park, Sung Wook; We, Jeoung Soon; Kim, Gye Won; Choi, Seong Hak; Park, Haeng Soon

    2002-02-01

    The stability of DA-1131 to renal dipeptidase (RDPase) (EC 3.4.13.19) was compared with that of imipenem and meropenem by V(max)/K(m) ratios as an index of the enzyme's preference for substrates. Our results showed a decreasing order of imipenem (6.24), meropenem (2.41), and DA-1131 (1.39). The biochemical evaluation of DA-1131 as the least preferred substrate of RDPase suggests its potential use as a novel beta-lactam antibiotic which may be usable without coadministration of RDPase inhibitors once its clinical suitability is proven. PMID:11796382

  12. Arbitrary Amplitude DIA and DA Solitary Waves in Adiabatic Dusty Plasmas

    SciTech Connect

    Mamun, A. A.; Jahan, N.; Shukla, P. K.

    2008-10-15

    The dust-ion-acoustic (DIA) as well as the dust-acoustic (DA) solitary waves (SWs) in an adiabatic dusty plasma are investigated by the pseudo-potential approach which is valid for arbitrary amplitude SWs. The role of the adiabaticity of electrons and ions in modifying the basic features (polarity, speed, amplitude and width) of arbitrary amplitude DIA and DA SWs are explicitly examined. It is found that the effects of the adiabaticity of electrons and ions significantly modify the basic features (polarity, speed, amplitude and width) of the DIA and DA SWs. The implications of our results in space and laboratory dusty plasmas are briefly discussed.

  13. 2012 DA14 and Chelyabinsk: Same Day Coincidence?

    NASA Astrophysics Data System (ADS)

    Chapman, Clark R.

    2013-10-01

    A long anticipated near-miss by near-Earth asteroid (NEA) 2012 DA14 on 15 February 2013 was upstaged 16 hours earlier by the impact and atmospheric explosion of a ~20 m NEA over Chelyabinsk, Russia. DA14 was earlier estimated to be 45 m across and passage at a distance under geosynchronous satellites was considered to be a record close approach by an object of that size, a once-in-40-years event. Actual Earth impact by a 20 m NEA had been estimated to be a once-in-200-years event. A simplistic calculation gives a probability of these happening on the same day of 1-in-a-billion. Within hours of the Chelyabinsk impact, it was recognized and widely reported that the two asteroids were in very different orbits and could not, at least in any readily understandable way, be physically related to each other (e.g. fragments of the same precursor body). Also, some early reports suggested that the two asteroids had very different compositions, further undermining the possibility they were pieces of the same body. (It seems unlikely, though certainly conceivable, that the two NEAs could have different compositions yet have some kind of causal connection resulting in the same-day events.) Nevertheless, incredibly tiny probabilities beg for an explanation. Here, with the benefit of hindsight, I re-examine issues that affect the probabilities, such as size, albedo, probable composition, and numbers of NEAs of these sizes. I also consider difficult issues of framing the apparent coincidence, which dominate other uncertainties. Updated information about the physical nature of the two asteroids somewhat modifies the original estimates of probabilities. Moreover, more proper ways of framing elements of the coincidence considerably reduce the improbability of the same-day events, but not nearly to the level (e.g. 1-in-1000 or perhaps 1-in-10,000) where we could rationalize dismissing the events as “just a coincidence.” The events of 15 February 2013 deserve more sophisticated

  14. Near-UV absorption in very cool DA white dwarfs

    SciTech Connect

    Saumon, D.; Holberg, J. B.; Kowalski, P. M. E-mail: holberg@argus.lpl.arizona.edu

    2014-07-20

    The atmospheres of very cool, hydrogen-rich white dwarfs (WDs) (T{sub eff} < 6000 K) are challenging to model because of the increased complexity of the equation of state, chemical equilibrium, and opacity sources in a low-temperature, weakly ionized dense gas. In particular, many models that assume relatively simple models for the broadening of atomic levels and mostly ideal gas physics overestimate the flux in the blue part of their spectra. A solution to this problem that has met with some success is that additional opacity at short wavelengths comes for the extreme broadening of the Lyman α line of atomic H by collisions primarily with H{sub 2}. For the purpose of validating this model more rigorously, we acquired Hubble Space Telescope STIS spectra of eight very cool WDs (five DA and three DC stars). Combined with their known parallaxes, BVRIJHK, and Spitzer IRAC photometry, we analyze their entire spectral energy distribution (from 0.24 to 9.3 μm) with a large grid of model atmospheres and synthetic spectra. We find that the red wing of the Lyman α line reproduces the rapidly decreasing near-UV flux of these very cool stars very well. We determine better constrained values of T{sub eff} and gravity as well as upper limits to the helium abundance in their atmospheres.

  15. Granite geomorphology and its geological controls, Serra da Estrela, Portugal

    NASA Astrophysics Data System (ADS)

    Migoń, Piotr; Vieira, Gonçalo

    2014-12-01

    Serra da Estrela is an elevated granite massif in central Portugal, characterized by extensive plateau surfaces incised by deep valleys affected by Quaternary glaciation, bounded by steep fault-generated escarpments. The presence of seven major textural variants of granite provides an opportunity to study the relationships between lithology and relief, whereas DEM analysis helped to show the relationships between lithology and topography objectively. The higher ground is associated with fine- to medium-grained granites and is typified by planar surfaces of low gradient, with occasional angular tors and rock pedestals. Block fields built by angular material are common in the parts that were not previously glaciated. Less elevated parts of the plateau are supported by medium- to coarse-grained granites and show more varied topography, with an abundance of tors, boulder piles, and depressions. Lithological boundaries locally coincide with slope breaks but this is not the rule. In the northern part of the massif a deep topographic basin has evolved in biotite granite, whereas deeply incised valleys follow major fault lines. Geological controls show a hierarchy, in that gross relief reflects the pattern of tectonic uplift and subsidence, whereas lithology and then fracture patterns become more and more important if one focuses on smaller and smaller landforms.

  16. D.A. Henderson: acting globally, thinking locally.

    PubMed

    Henderson, D A

    2003-08-01

    They had said that it couldn't be done-the worldwide eradication of smallpox. To hear D.A. Henderson tell it, the job of leading the World Health Organization's initiative to conquer the disease in the 1960s and 1970s rather fell into his lap. In fact, he describes each of the posts that he has held with great modesty, beginning with his military service at the Centers for Disease Control and Prevention all the way through his assignments as Dean of Public Health at Johns Hopkins, Associate Director of the Office of Science and Technology Policy in the Executive Office of the President, and more recently as Director of the Office of Public Health Preparedness under Secretary Thompson at the Department of Health and Human Services. Confronted with enormous challenges in terms of public health initiatives, Henderson describes each assignment as a matter of communicating with the people he works with and the people that he serves, and drawing on their insights to devise strategies for accomplishing the task at hand. With bioterrorism posing one of the major public health concerns to face the United States and the world, it's gratifying to know that someone with Henderson's track record and wide-ranging expertise is paying attention and making sure that medical and government officials are preparing to respond to the threat. Again and again, Henderson appears to have the knack for showing up in the right place at the right time with just the right idea. PMID:14993437

  17. Robotic Partial Nephrectomy with the Da Vinci Xi

    PubMed Central

    Kallingal, George J. S.; Swain, Sanjaya; Darwiche, Fadi; Punnen, Sanoj; Manoharan, Murugesan; Gonzalgo, Mark L.; Parekh, Dipen J.

    2016-01-01

    Purpose. The surgical expertise to perform robotic partial nephrectomy is heavily dependent on technology. The Da Vinci Xi (XI) is the latest robotic surgical platform with significant advancements compared to its predecessor. We describe our operative technique and experience with the XI system for robotic partial nephrectomy (RPN). Materials and Methods. Patients with clinical T1 renal masses were offered RPN with the XI. We used laser targeting, autopositioning, and a novel “in-line” port placement to perform RPN. Results. 15 patients underwent RPN with the XI. There were no intraoperative complications and no operative conversions. Mean console time was 101.3 minutes (range 44–176 minutes). Mean ischemia time was 17.5 minutes and estimated blood loss was 120 mLs. 12 of 15 patients had renal cell carcinoma. Two patients had oncocytoma and one had benign cystic disease. All patients had negative surgical margins and pathologic T1 disease. Two postoperative complications were encountered, including one patient who developed a pseudoaneurysm and one readmitted for presumed urinary tract infection. Conclusions. RPN with the XI system can be safely performed. Combining our surgical technique with the technological advancements on the XI offers patients acceptable pathologic and perioperative outcomes. PMID:26977144

  18. Audio annotation watermarking with robustness against DA/AD conversion

    NASA Astrophysics Data System (ADS)

    Qian, Kun; Kraetzer, Christian; Biermann, Michael; Dittmann, Jana

    2010-01-01

    In the paper we present a watermarking scheme developed to meet the specific requirements of audio annotation watermarking robust against DA/AD conversion (watermark detection after playback by loudspeaker and recording with a microphone). Additionally the described approach tries to achieve a comparably low detection complexity, so it could be embedded in the near future in low-end devices (e.g. mobile phones or other portable devices). We assume in the field of annotation watermarking that there is no specific motivation for attackers to the developed scheme. The basic idea for the watermark generation and embedding scheme is to combine traditional frequency domain spread spectrum watermarking with psychoacoustic modeling to guarantee transparency and alphabet substitution to improve the robustness. The synchronization and extraction scheme is designed to be much less computational complex than the embedder. The performance of the scheme is evaluated in the aspects of transparency, robustness, complexity and capacity. The tests reveals that 44% out of 375 tested audio files pass the simulation test for robustness, while the most appropriate category shows even 100% robustness. Additionally the introduced prototype shows an averge transparency of -1.69 in SDG, while at the same time having a capacity satisfactory to the chosen application scenario.

  19. DA WHITE DWARFS OBSERVED IN THE LAMOST PILOT SURVEY

    SciTech Connect

    Zhang Yueyang; Deng Licai; Liu Chao; Carrell, Kenneth; Yang Fan; Gao Shuang; Xu Yan; Li Jing; Zhang Haotong; Zhao Yongheng; Luo Ali; Bai Zhongrui; Yuan Hailong; Lepine, Sebastien; Newberg, Heidi Jo; Carlin, Jeffrey L.; Jin Ge

    2013-08-01

    A total of {approx}640, 000 objects from the LAMOST pilot survey have been publicly released. In this work, we present a catalog of DA white dwarfs (DAWDs) from the entire pilot survey. We outline a new algorithm for the selection of white dwarfs (WDs) by fitting Sersic profiles to the Balmer H{beta}, H{gamma}, and H{delta} lines of the spectra, and calculating the equivalent width of the Ca II K line. Two thousand nine hundred sixty-four candidates are selected by constraining the fitting parameters and the equivalent width of the Ca II K line. All the spectra of candidates are visually inspected. We identify 230 DAWDs (59 of which are already included in the Villanova and SDSS WD catalogs), 20 of which are DAWDs with non-degenerate companions. In addition, 128 candidates are classified as DAWDs/subdwarfs, which means the classifications are ambiguous. The result is consistent with the expected DAWD number estimated based on the LEGUE target selection algorithm.

  20. Adenovirus type 2 encoded early 11 kDa protein

    SciTech Connect

    Murthy, S.V.K.N.; Kapoor, Q.S.

    1986-05-01

    Several adenovirus type 2 (Ad2) encoded early proteins have been identified in viral infected human KB cells. These proteins are of great interest as they play key roles in cell transformation, viral DNA synthesis and gene expression. They have partially purified an AD2 encoded early polypeptide of an apparent molecular weight of 11 kilodaltons from the nuclei of viral infected cells labelled with /sup 35/S-methionine. After DNA removal from the nuclear extracts, the polypeptide was isolated using DEAE-Sephacel anion exchange and Biogel P-10 gel filtration columns. This simple two step procedure yielded several fold purification of the polypeptide. Antisera raised in mice against an Ad2 transformed rat cell line 8617 was found to immunoprecipitate the 11 kDa polypeptide from the nuclear extract of Ad2 infected KB cells. After relating this protein to an open reading frame of an Ad2 early gene block by matching the amino acid sequences to the nucleotide sequences of early genes, they plan to functionally characterize this protein by using monoclonal antibodies in in vivo and in vitro experiments.

  1. Biogeochemical Indicators of Paleoenvironmental Changes in a Humid Region of Amazonia (Lagoa da Pata, Sao Gabriel da Cachoeira)

    NASA Astrophysics Data System (ADS)

    Cordeiro, R. C.; Turcq, B.; Mendes, L. G.; Azeredo, J. B.; Moura, M. S.; de Oliveira, A. R.; Monteiro, F.; Santelli, R. E.

    2004-12-01

    Analysis of four sediment core collected in Lagoa da Pata lake show significant changes in the environmental history of Amazonia during the last 40,000 years. The Lagoa da Pata Lake (0°17'S, 66°40'W) is located on the top of the Morro dos Seis Lagos, an isolated hill in the extensive forested plane surface of high Rio Negro basin. The climate in the region presents mean annual precipitation around 3000 mm without dry season. Three distinct sections are clearly identified in the LPT III, LPT IV and LPT V cores. They consist of upper and lower organic-rich layers, separated by a clastic layer which represents a short period of rapid accumulation around 18 ka BP. Analysis of the organic matter composition by d13C and d15N, total Carbon and Nitrogen, sedimentary chlorophyll concentration and organic matter petrography show three different phases related to organic matter deposition. Between, at least 46,000 and 31,000 14C yr B.P., the lake presented a high water level attested by high accumulation rates of organic carbon. Values of d13C (around -32%) associated to high C/N ratio demonstrate a lignocellulosic material contribution in this phase; the second phase between the 31000 yr 14C B.P. and 18000 14C B.P. is characterised by a lower productivity attested by low sedimentary chlorophyll and total organic carbon values. The d13C values increase with medium values around -28% that may correspond to an input of organic matter rich in C4 plants (grasses). The sedimentation rate decreases significantly. High charcoal fluxes were observed in this period. At around 18000 14C yr BP, there appears to have been a sudden input of clastic material. This is represented by a sandy facies, which exhibits lower carbon contents. From 18000 14C yr B.P. to the present time occurred an increase in lacustrine productivity marked by an increase in carbon and chlorophyll derivate accumulation rates. The C/N values dropped indicting an algae organic matter contribution. It probably

  2. Temperature and Gravity Dependence of Trace Element Abundances in Hot DA White Dwarfs (94-EUVE-094)

    NASA Technical Reports Server (NTRS)

    Finley, David S.

    1998-01-01

    EUV spectroscopy has shown that DA white dwarfs hotter than about 45,000 K may contain trace heavy elements, while those hotter than about 50,000 K almost always have significant abundances of trace heavy elements. One of our continuing challenges is to identify and determine the abundances of these trace constituents, and then to relate the observed abundance patterns to the present conditions and previous evolutionary histories of the hot DA white dwarfs.

  3. The 32 kDa Enamelin Undergoes Conformational Transitions upon Calcium Binding

    PubMed Central

    Fan, Daming; Lakshminarayanan, Rajamani; Moradian-Oldak, Janet

    2008-01-01

    The 32 kDa hydrophilic and acidic enamelin, the most stable cleavage fragment of the enamel specific glycoprotein, is believed to play vital roles in controlling crystal nucleation or growth during enamel biomineralization. Circular dichroism and Fourier transform infrared spectra demonstrate that the secondary structure of the 32 kDa enamelin has a high content of α-helix (81.5%). Quantitative analysis on the circular dichroism data revealed that the 32 kDa enamelin undergoes conformational changes with a structural preference to β-sheet as a function of calcium ions. We suggest that the increase of β-sheet conformation upon presence of Ca2+ may allow preferable interaction of the 32 kDa enamelin with apatite crystal surfaces during enamel biomineralization. The calcium association constant of the 32 kDa enamelin calculated from the fitting curve of ellipticity at 222 nm is Ka = 1.55 (±0.13) × 103 M−1, indicating a relatively low affinity. Our current biophysical studies on the 32 kDa enamelin structure provide novel insights towards understanding the enamelin-mineral interaction and subsequently the functions of enamelin during enamel formation. PMID:18508280

  4. OpenDA: Open Source Generic Data Assimilation Environment and its Application in Geophysical Process Models

    NASA Astrophysics Data System (ADS)

    Weerts, A.; van Velzen, N.; Verlaan, M.; Sumihar, J.; Hummel, S.; El Serafy, G.; Dhondia, J.; Gerritsen, H.; Vermeer-Ooms, S.; Loots, E.; Markus, A.; Kockx, A.

    2011-12-01

    Data Assimilation techniques are essential elements in state-of-the-art development of models and their use in operational forecasting and real-time control in the fields of groundwater, surface water and soil systems. In meteorological and atmospheric sciences, steady improvements in numerical weather forecasting and climate prediction over the last couple of decades have been enabled to a large degree by the development of community-based models and data assimilation systems. The hydrologic community should learn from the experiences of the meteorological and atmospheric communities by accelerating the transition of hydrologic DA research into operations and developing community-supported, open-source modeling and forecasting systems and data assimilation tools. In 2010, a community based open source initiative named OpenDA was started. The openDA initiative bears similarities with the well-known openMI initiative. OpenDA makes use of a set of interfaces that describe the interaction between models, observations and data assimilation algorithms. It focuses on flexible applications in portable systems for modeling geophysical processes. It provides a generic interfacing protocol that allows combination of the implemented data assimilation techniques with, in principle, any time-stepping model describing a process (atmospheric processes, 3D circulation, 2D water flow, rainfall-runoff, unsaturated flow, groundwater flow, etc.). Presently, openDA features filtering techniques and calibration techniques. The presentation will give an overview of openDA and the results of some of its practical applications.

  5. In vitro degradation of the 32kDa PS II reaction centre protein

    SciTech Connect

    Eckenswiller, L.C.; Greenberg, B.M. )

    1989-04-01

    The 32kDa thylakoid membrane protein is an integral component of the PS II reaction centre. The protein, although stable in the dark, undergoes light dependent turnover. Light from the UV, visible and far-red spectral regions induce 32kDa protein degradation. To better understand 32kDa protein metabolism, an in vitro degradation system is being developed. It consists of isolated thylakoid membranes than contain radiolabelled protein. The 32kDa protein is actively and specifically degraded when the thylakoid preparation is exposed to UV or visible radiation. The protein is stable in the dark. The herbicides (atrazine and DCMU) inhibit degradation in the in vitro system as they do in vivo. Additionally, several methods of isolating thylakoids are being compared to optimize the 32kDa protein degradation reaction. The preparations will be evaluated based on their ability to permit light dependent degradation of the 32kDa protein without affecting the other membrane components.

  6. [The behavior of superoxide dismutase (SOD) in serum of cows with abomasal displacement (DA)].

    PubMed

    Fürll, M; Dabbagh, M N; Fürll, B; Sattler, T

    2004-01-01

    After surgical reposition of displaced organs (abomasum, uterus, intestines) restoration of blood flow and oxygen supply generates oxygen radicals and other reactive oxygen species. SOD indicates radical stress of the organism. Subject of the study was the question if SOD can be detected in blood serum samples of cows and if there are differences in SOD activity between healthy cows and cows with Dislocatio abomasi (DA). We also wanted to investigate the influence of breed "Schwarzbunte" with DA (16 left/5 rights). The samples were drawn before and 1, 3 and 24 post op. Ten healthy cows of the same breed were also examined (2 weeks and 4-6 weeks after calving). There are no significant differences between the SOD activity of healthy cows and cows with DA, but the SOD activity of cows with left DA is significant lower than the activity of cows with right DA. Post op. SOD activity decreases; 24 h after surgery cows with left but not with right DA show an increase of SOD activity similar to values before surgery. There is a close positive correlation between SOD activity and protein concentration as well as negative correlation to concentration of free fatty acids after surgery. The behaviour of SOD activity shows that the surgical replacement of the displaced abomasum can generate a depression of the antioxidative capacity of the organism. PMID:14983749

  7. The sandfly fauna (Diptera: Psychodidae: Phlebotominae) of the Parque Estadual da Serra da Tiririca, Rio de Janeiro, Brazil

    PubMed Central

    Rodrigues, Andressa Alencastre Fuzari; Barbosa, Vanessa de Araújo; Andrade, José Dilermando; Brazil, Reginaldo Peçanha

    2013-01-01

    Cutaneous leishmaniasis (CL) in the state of Rio de Janeiro is sporadic and can be characterised as a peridomestic transmission that occurs in modified natural environments. The aim of this work was to study the fauna and ecological characteristics of sandflies in an environmentally protected area (the State Park of Serra da Tiririca) within the remnants of the Atlantic Forest in the municipalities of Niterói and Maricá and their possible relationship with leishmaniasis. Captures were performed using light traps during the night once a month for one year in both sylvatic environments and areas surrounding homes near the park. A total of 1,037 sandflies were captured, belonging to nine genera and 12 species: Evandromyia tupynambai (34.1%), Migonemyia migonei (20.6%), Brumptomyia cunhai (13.8%), Micropygomyia schreiberi (9.7%), Psathyromyia lanei (6.5%), Brumptomyia nitzulescui (5.7%), Evandromyia edwardsi (5.4%), Nyssomyia intermedia (2.8%), Evandromyia cortelezzii (0.6%), Pintomyia bianchigalatiae (0.5%), Lutzomyia longipalpis (0.2%) and Sciopemyia microps (0.1%). Both Mg. migonei and Ny. intermedia may be acting as vectors of CL in this area. PMID:24141956

  8. Negative regulation of human immunodeficiency virus type 1 expression in monocytes: role of the 65-kDa plus 50-kDa NF-kappa B dimer.

    PubMed

    Raziuddin; Mikovits, J A; Calvert, I; Ghosh, S; Kung, H F; Ruscetti, F W

    1991-11-01

    Although monocytic cells can provide a reservoir for viral production in vivo, their regulation of human immunodeficiency virus type 1 (HIV-1) transcription can be either latent, restricted, or productive. These differences in gene expression have not been molecularly defined. In THP-1 cells with restricted HIV expression, there is an absence of DNA-protein binding complex formation with the HIV-1 promoter-enhancer associated with markedly less viral RNA production. This absence of binding was localized to the NF-kappa B region of the HIV-1 enhancer; the 65-kDa plus 50-kDa NF-kappa B heterodimer was preferentially lost. Adding purified NF-kappa B protein to nuclear extracts from cells with restricted expression overcomes this lack of binding. In addition, treatment of these nuclear extracts with sodium deoxycholate restored their ability to form the heterodimer, suggesting the presence of an inhibitor of NF-kappa B activity. Furthermore, treatment of nuclear extracts from these cells that had restricted expression with lipopolysaccharide increased viral production and NF-kappa B activity. Antiserum specific for NF-kappa B binding proteins, but not c-rel-specific antiserum, disrupted heterodimer complex formation. Thus, both NF-kappa B-binding complexes are needed for optimal viral transcription. Binding of the 65-kDa plus 50-kDa heterodimer to the HIV-1 enhancer can be negatively regulated in monocytes, providing one mechanism restricting HIV-1 gene expression. PMID:1946356

  9. Myelin management by the 18.5–kDa and 21.5–kDa classic myelin basic protein isoforms

    PubMed Central

    Harauz, George; Boggs, Joan M.

    2013-01-01

    The classic myelin basic protein (MBP) splice isoforms range in nominal molecular mass from 14 to 21.5 kDa, and arise from the gene in the oligodendrocyte lineage (Golli) in maturing oligodendrocytes. The 18.5-kDa isoform that predominates in adult myelin adheres the cytosolic surfaces of oligodendrocyte membranes together, and forms a two-dimensional molecular sieve restricting protein diffusion into compact myelin. However, this protein has additional roles including cytoskeletal assembly and membrane extension, binding to SH3-domains, participation in Fyn-mediated signaling pathways, sequestration of phosphoinositides, and maintenance of calcium homeostasis. Of the diverse post-translational modifications of this isoform, phosphorylation is the most dynamic, and modulates 18.5-kDa MBP’s protein-membrane and protein-protein interactions, indicative of a rich repertoire of functions. In developing and mature myelin, phosphorylation can result in microdomain or even nuclear targeting of the protein, supporting the conclusion that 18.5-kDa MBP has significant roles beyond membrane adhesion. The full-length, early-developmental 21.5-kDa splice isoform is predominantly karyophilic due to a non-traditional P-Y nuclear localization signal, with effects such as promotion of oligodendrocyte proliferation. We discuss in vitro and recent in vivo evidence for multifunctionality of these classic basic proteins of myelin, and argue for a systematic evaluation of the temporal and spatial distributions of these protein isoforms, and their modified variants, during oligodendrocyte differentiation. PMID:23398367

  10. Characteristics of 26 kDa antigen of H. Pylori by Monoclonal Antibody.

    PubMed

    Ghahremani, Hossein; Farshad, Shohreh; Amini Najafabadi, Hossein; Kashanian, Susan; Momeni Moghaddam, Mohammad Amin; Moradi, Nariman; Paknejad, Maliheh

    2015-02-01

    Alkylhydroperoxide reductase (AhpC, the 26 kDa antigen) is one of the abundant antioxidant enzymes in Helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect H. pylori infection in clinical specimens. This study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. Five established hybridoma cell lines secreting monoclonal antibodies (MAbs) against 26 kDa antigen of H. pylori were cultivated and MAbs were purified by affinity chromatography. Subsequently, MAbs were conjugated with biotin, and different combinations of capture and tracer antibodies used in sandwich ELISA. Immunoblotting of bacterial extracts were performed to estimate aggregation status of the antigen. Release of antigen from the cultivated bacteria on solid media was examined by sandwich ELISA, and also, existence of interference in fecal extract was investigated by immunoblotting and sandwich ELISA. Our findings showed that the MAbs against 26 kDa antigen of H. pylori could recognize three bands of nearly 25 kDa, 50 kDa, and 75 kDa in immunoblotting. This study also indicated presence of more antigens in the culture medium around the bacteria than the bacterial extract itself. The results of sandwich ELISA and immunoblotting on fecal extracts suggest the presence of interfering agents that prevent detection of antigen by antibody in ELISA but not in immunoblotting. In this study the oligomerization of the 26 kDa antigen, presence of interfering agents in stool matrix, and release of antigen to outside of bacteria, were demonstrated. PMID:25530147

  11. Naloxone-induced jumping activity in morphine-dependent mice: a pharmacological study on the role of DA2 receptors.

    PubMed

    Cerbo, R; Boni, B; De Lena, C; Cesarino, F; Meco, G; Feliciani, M; Formisano, R; Casacchia, M

    1984-03-30

    The morphine withdrawal syndrome is strictly related to modifications on neurotransmittorial systems at central and peripheral levels. Dopamine seems to play an important role. Particularly in the experimental withdrawal syndrome the jumping activity seems to be related to a hypersensitivity of DA receptors. While the role of DA1 receptors has been extensively studied, using agonist and antagonist drugs, the role of DA2 receptors is still little known. In our study we evaluated the changes of the jumping response that was induced by substances active on DA2 receptors (lisuride, tiapride) in mice made acutely dependent on morphine, in which we induced a withdrawal syndrome with naloxone. Haloperidol (DA1-DA2 blocker) did not provoke significant changes. Either tiapride or lisuride determined a sharp increase of jumping. PMID:6712813

  12. 11 CFR 111.11 - Written questions under order (2 U.S.C. 437d(a)(1)).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 11 Federal Elections 1 2011-01-01 2011-01-01 false Written questions under order (2 U.S.C. 437d(a... PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111.11 Written questions under order (2 U.S.C. 437d(a)(1... submit sworn written answers to written questions and may specify a date by which such answers must...

  13. 11 CFR 111.11 - Written questions under order (2 U.S.C. 437d(a)(1)).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 11 Federal Elections 1 2010-01-01 2010-01-01 false Written questions under order (2 U.S.C. 437d(a... PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111.11 Written questions under order (2 U.S.C. 437d(a)(1... submit sworn written answers to written questions and may specify a date by which such answers must...

  14. 82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.

    PubMed

    Winick-Ng, Warren; Caetano, Fabiana A; Winick-Ng, Jennifer; Morey, Trevor M; Heit, Bryan; Rylett, R Jane

    2016-01-01

    The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1-42 (Aβ1-42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1-42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generation sequencing (ChIP-seq), we identified that Aβ1-42-exposure increases 82-kDa ChAT association with gene promoters and introns. The Aβ1-42-induced 82-kDa ChAT aggregates co-localize with special AT-rich binding protein 1 (SATB1), which anchors DNA to scaffolding/matrix attachment regions (S/MARs). SATB1 had a similar genomic association as 82-kDa ChAT, with both proteins associating with synapse and cell stress genes. After Aβ1-42 -exposure, both SATB1 and 82-kDa ChAT are enriched at the same S/MAR on the APP gene, with 82-kDa ChAT expression attenuating an increase in an isoform-specific APP mRNA transcript. Finally, 82-kDa ChAT and SATB1 have patterned genomic association at regions enriched with S/MAR binding motifs. These results demonstrate that 82-kDa ChAT and SATB1 play critical roles in the response of neural cells to acute Aβ-exposure. PMID:27052102

  15. An Introduction to the DA-T Gibbs Sampler for the Two-Parameter Logistic (2PL) Model and beyond

    ERIC Educational Resources Information Center

    Maris, Gunter; Bechger, Timo M.

    2005-01-01

    The DA-T Gibbs sampler is proposed by Maris and Maris (2002) as a Bayesian estimation method for a wide variety of "Item Response Theory (IRT) models". The present paper provides an expository account of the DA-T Gibbs sampler for the 2PL model. However, the scope is not limited to the 2PL model. It is demonstrated how the DA-T Gibbs sampler for…

  16. 82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions

    PubMed Central

    Winick-Ng, Warren; Caetano, Fabiana A.; Winick-Ng, Jennifer; Morey, Trevor M.; Heit, Bryan; Rylett, R. Jane

    2016-01-01

    The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1–42 (Aβ1–42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1–42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generation sequencing (ChIP-seq), we identified that Aβ1–42 -exposure increases 82-kDa ChAT association with gene promoters and introns. The Aβ1–42 -induced 82-kDa ChAT aggregates co-localize with special AT-rich binding protein 1 (SATB1), which anchors DNA to scaffolding/matrix attachment regions (S/MARs). SATB1 had a similar genomic association as 82-kDa ChAT, with both proteins associating with synapse and cell stress genes. After Aβ1–42 -exposure, both SATB1 and 82-kDa ChAT are enriched at the same S/MAR on the APP gene, with 82-kDa ChAT expression attenuating an increase in an isoform-specific APP mRNA transcript. Finally, 82-kDa ChAT and SATB1 have patterned genomic association at regions enriched with S/MAR binding motifs. These results demonstrate that 82-kDa ChAT and SATB1 play critical roles in the response of neural cells to acute Aβ -exposure. PMID:27052102

  17. High resolution structure of cleaved Serpin 42 Da from Drosophila melanogaster

    PubMed Central

    2014-01-01

    Background The Drosophila melanogaster Serpin 42 Da gene (previously Serpin 4) encodes a serine protease inhibitor that is capable of remarkable functional diversity through the alternative splicing of four different reactive centre loop exons. Eight protein isoforms of Serpin 42 Da have been identified to date, targeting the protease inhibitor to both different proteases and cellular locations. Biochemical and genetic studies suggest that Serpin 42 Da inhibits target proteases through the classical serpin ‘suicide’ inhibition mechanism, however the crystal structure of a representative Serpin 42 Da isoform remains to be determined. Results We report two high-resolution crystal structures of Serpin 42 Da representing the A/B isoforms in the cleaved conformation, belonging to two different space-groups and diffracting to 1.7 Å and 1.8 Å. Structural analysis reveals the archetypal serpin fold, with the major elements of secondary structure displaying significant homology to the vertebrate serpin, neuroserpin. Key residues known to have central roles in the serpin inhibitory mechanism are conserved in both the hinge and shutter regions of Serpin 42 Da. Furthermore, these structures identify important conserved interactions that appear to be of crucial importance in allowing the Serpin 42 Da fold to act as a versatile template for multiple reactive centre loops that have different sequences and protease specificities. Conclusions In combination with previous biochemical and genetic studies, these structures confirm for the first time that the Serpin 42 Da isoforms are typical inhibitory serpin family members with the conserved serpin fold and inhibitory mechanism. Additionally, these data reveal the remarkable structural plasticity of serpins, whereby the basic fold is harnessed as a template for inhibition of a large spectrum of proteases by reactive centre loop exon ‘switching’. This is the first structure of a Drosophila serpin reported to date

  18. Characterization of the 105-kDa molecular chaperone. Identification, biochemical properties, and localization.

    PubMed

    Matsumori, Mika; Itoh, Hideaki; Toyoshima, Itaru; Komatsuda, Atsushi; Sawada, Ken-Ichi; Fukuda, Jun; Tanaka, Toshinobu; Okubo, Atsuya; Kinouchi, Hiroyuki; Mizoi, Kazuo; Hama, Tokiko; Suzuki, Akira; Hamada, Fumio; Otaka, Michiro; Shoji, Yutaka; Takada, Goro

    2002-11-01

    We have characterized the biochemical properties of the testis and brain-specific 105-kDa protein which is cross-reacted with an anti-bovine HSP90 antibody. The protein was induced in germ cells by heat stress, resulting in a protein which is one of the heat shock proteins [Kumagai, J., Fukuda, J., Kodama, H., Murata, M., Kawamura, K., Itoh, H. & Tanaka, T. (2000) Eur. J. Biochem.267, 3073-3078]. In the present study, we characterized the biochemical properties of the protein. The 105-kDa protein inhibited the aggregation of citrate synthase as a molecular chaperone in vitro. ATP/MgCl2 has a slight influence of the suppression of the citrate synthase aggregation by the 105-kDa protein. The protein possessed chaperone activity. The protein was able to bind to ATP-Sepharose like the other molecular chaperone HSP70. A partial amino-acid sequence (24 amino-acid residues) of the protein was determined and coincided with those of the mouse testis- and brain-specific APG-1 and osmotic stress protein 94 (OSP94). The 105-kDa protein was detected only in the medulla of the rat kidney sections similar to OSP94 upon immunoblotting. The purified 105-kDa protein was cross-reacted with an antibody against APG-1. These results suggested that APG-1 and OSP94 are both identical to the 105-kDa protein. There were highly homologous regions between the 105-kDa protein/APG-1/OSP94 and HSP90. The region of HSP90 was also an immunoreactive site. An anti-bovine HSP90 antibody may cross-react with the 105-kDa protein similar to HSP90 in the rat testis and brain. We have investigated the localization and developmental induction of the protein in the rat brain. In the immunohistochemical analysis, the protein was mainly detected in the cytoplasm of the nerve and glial cells of the rat brain. Although the 105-kDa protein was localized in all rat brain segments, the expression pattern was fast in the cerebral cortex and hippocampus and slow in the cerebellum. PMID:12423363

  19. The rare DAT coding variant Val559 perturbs DA neuron function, changes behavior, and alters in vivo responses to psychostimulants.

    PubMed

    Mergy, Marc A; Gowrishankar, Raajaram; Gresch, Paul J; Gantz, Stephanie C; Williams, John; Davis, Gwynne L; Wheeler, C Austin; Stanwood, Gregg D; Hahn, Maureen K; Blakely, Randy D

    2014-11-01

    Despite the critical role of the presynaptic dopamine (DA) transporter (DAT, SLC6A3) in DA clearance and psychostimulant responses, evidence that DAT dysfunction supports risk for mental illness is indirect. Recently, we identified a rare, nonsynonymous Slc6a3 variant that produces the DAT substitution Ala559Val in two male siblings who share a diagnosis of attention-deficit hyperactivity disorder (ADHD), with other studies identifying the variant in subjects with bipolar disorder (BPD) and autism spectrum disorder (ASD). Previously, using transfected cell studies, we observed that although DAT Val559 displays normal total and surface DAT protein levels, and normal DA recognition and uptake, the variant transporter exhibits anomalous DA efflux (ADE) and lacks capacity for amphetamine (AMPH)-stimulated DA release. To pursue the significance of these findings in vivo, we engineered DAT Val559 knock-in mice, and here we demonstrate in this model the presence of elevated extracellular DA levels, altered somatodendritic and presynaptic D2 DA receptor (D2R) function, a blunted ability of DA terminals to support depolarization and AMPH-evoked DA release, and disruptions in basal and psychostimulant-evoked locomotor behavior. Together, our studies demonstrate an in vivo functional impact of the DAT Val559 variant, providing support for the ability of DAT dysfunction to impact risk for mental illness. PMID:25331903

  20. The rare DAT coding variant Val559 perturbs DA neuron function, changes behavior, and alters in vivo responses to psychostimulants

    PubMed Central

    Mergy, Marc A.; Gowrishankar, Raajaram; Gresch, Paul J.; Gantz, Stephanie C.; Williams, John; Davis, Gwynne L.; Wheeler, C. Austin; Stanwood, Gregg D.; Hahn, Maureen K.; Blakely, Randy D.

    2014-01-01

    Despite the critical role of the presynaptic dopamine (DA) transporter (DAT, SLC6A3) in DA clearance and psychostimulant responses, evidence that DAT dysfunction supports risk for mental illness is indirect. Recently, we identified a rare, nonsynonymous Slc6a3 variant that produces the DAT substitution Ala559Val in two male siblings who share a diagnosis of attention-deficit hyperactivity disorder (ADHD), with other studies identifying the variant in subjects with bipolar disorder (BPD) and autism spectrum disorder (ASD). Previously, using transfected cell studies, we observed that although DAT Val559 displays normal total and surface DAT protein levels, and normal DA recognition and uptake, the variant transporter exhibits anomalous DA efflux (ADE) and lacks capacity for amphetamine (AMPH)-stimulated DA release. To pursue the significance of these findings in vivo, we engineered DAT Val559 knock-in mice, and here we demonstrate in this model the presence of elevated extracellular DA levels, altered somatodendritic and presynaptic D2 DA receptor (D2R) function, a blunted ability of DA terminals to support depolarization and AMPH-evoked DA release, and disruptions in basal and psychostimulant-evoked locomotor behavior. Together, our studies demonstrate an in vivo functional impact of the DAT Val559 variant, providing support for the ability of DAT dysfunction to impact risk for mental illness. PMID:25331903

  1. A Influência da Escola e da Mídia na Obtenção dos Conhecimentos Astronômicos

    NASA Astrophysics Data System (ADS)

    da Cunha, W. S.; Voelzke, M. R.; Amaral, L. H.

    2006-08-01

    O universo em que vivemos possui segredos jamais imaginados pelo homem, fruto da pequena proporção que ocupamos diante de um cosmos infinito. A astronomia funciona como um instrumento que sacia a sede humana em desvendar esses segredos, estudando as galáxias, quasares, pulsares além da existência de todos os elementos que preenchem o espaço cósmico, o vácuo, não mais vazio como se pensou em outros tempos. A mídia explora esse campo de forma mística e também científica. A difusão adequada e dentro dos padrões educacionais vigentes, de conceitos astronômicos, se faz necessária, devido sua presença nos conteúdos escolares, centros de ciência e na mídia, de modo geral. A reforma do Currículo Básico da Escola Pública da maioria dos Estados brasileiros introduziu conceitos astronômicos desde a pré-escola até o Ensino Médio. Num estudo realizado com 1.032 alunos do Ensino Médio de seis escolas estaduais, revelou-se através da aplicação de um questionário que abordava conheciment os básicos em astronomia, como estes foram adquiridos e qual era a infra-estrutura oferecida pela escola: para 66,2% dos entrevistados o professor já fez alguma apresentação a respeito de astronomia. O presente estudo revelou também que 44,5% dos alunos entrevistados adquiriram seus conhecimentos astronômicos na escola, 28,1% através da televisão e 27,4% afirmaram que seus conhecimentos foram obtidos através de outros tipos de mídia (internet, filmes e revistas). Embora o estudo revele claramente que há certo equilíbrio na forma em que os alunos obtiveram seus conhecimentos astronômicos, a mídia ainda explora e muitas vezes desperta o interesse pela astronomia com assuntos que não fazem parte de nosso cotidiano como, por exemplo, o homem na Lua, os projetos espaciais e a primeira viagem de um astronauta brasileiro no espaço. Porém, em certos casos, a mídia pode promover conhecimentos incompletos ou mesmo equivocados sobre os conceitos que envolvam

  2. In vitro study on the interaction between the 32 kDa enamelin and amelogenin

    PubMed Central

    Fan, Daming; Du, Chang; Sun, Zhi; Lakshminarayanan, Rajamani; Moradian-Oldak, Janet

    2015-01-01

    Enamel extracelluar matrix components play vital roles in controlling crystal nucleation and growth during enamel formation. We investigated the interaction between the 32 kDa enamelin fragment and amelogenin using immunochemical and biophysical methods. Immunoprecipitation studies revealed that the 32 kDa enamelin and amelogenin eluted together from a Protein A column. Dynamic light scattering results showed that the 32 kDa enamelin had a profound effect on amelogenin assembly at pH 8.0, causing partial dissociation of the nanospheres, in a dose-dependent manner. The appearance of an isodichroic point and the shifting and intensity decrease of the ellipticity minima in the circular dichroism spectra of amelogenin following the addition of the 32 kDa enamelin were indicative of conformational changes in amelogenin and of a direct interaction between the two macromolecules. Our results collectively demonstrate that the 32 kDa enamelin has a direct interaction with amelogenin in vitro. Our current studies provide novel insights into understanding possible cooperation between enamelin and amelogenin in macromolecular self-assembly and in controlling enamel mineral formation. PMID:19263522

  3. Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron.

    PubMed

    Shen, Hung-Chang; Wei, Jia-Yi; Chu, Sao-Yu; Chung, Pei-Chi; Hsu, Tsai-Chi; Yu, Hung-Hsiang

    2016-01-01

    In the Drosophila olfactory system, odorant information is sensed by olfactory sensory neurons and relayed from the primary olfactory center, the antennal lobe (AL), to higher olfactory centers via olfactory projection neurons (PNs). A major portion of the AL is constituted with dendrites of four groups of PNs, anterodorsal PNs (adPNs), lateral PNs (lPNs), lateroventral PNs (lvPNs) and ventral PNs (vPNs). Previous studies have been focused on the development and function of adPNs and lPNs, while the investigation on those of lvPNs and vPNs received less attention. Here, we study the molecular and cellular mechanisms underlying the morphogenesis of a putative male-pheromone responding vPN, the DA1 vPN. Using an intersection strategy to remove background neurons labeled within a DA1 vPN-containing GAL4 line, we depicted morphological changes of the DA1 vPN that occurs at the pupal stage. We then conducted a pilot screen using RNA interference knock-down approach to identify cell surface molecules, including Down syndrome cell adhesion molecule 1 and Semaphorin-1a, that might play essential roles for the DA1 vPN morphogenesis. Taken together, by revealing molecular and cellular basis of the DA1 vPN morphogenesis, we should provide insights into future comprehension of how vPNs are assembled into the olfactory neural circuitry. PMID:27163287

  4. Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron

    PubMed Central

    Yu, Hung-Hsiang

    2016-01-01

    In the Drosophila olfactory system, odorant information is sensed by olfactory sensory neurons and relayed from the primary olfactory center, the antennal lobe (AL), to higher olfactory centers via olfactory projection neurons (PNs). A major portion of the AL is constituted with dendrites of four groups of PNs, anterodorsal PNs (adPNs), lateral PNs (lPNs), lateroventral PNs (lvPNs) and ventral PNs (vPNs). Previous studies have been focused on the development and function of adPNs and lPNs, while the investigation on those of lvPNs and vPNs received less attention. Here, we study the molecular and cellular mechanisms underlying the morphogenesis of a putative male-pheromone responding vPN, the DA1 vPN. Using an intersection strategy to remove background neurons labeled within a DA1 vPN-containing GAL4 line, we depicted morphological changes of the DA1 vPN that occurs at the pupal stage. We then conducted a pilot screen using RNA interference knock-down approach to identify cell surface molecules, including Down syndrome cell adhesion molecule 1 and Semaphorin-1a, that might play essential roles for the DA1 vPN morphogenesis. Taken together, by revealing molecular and cellular basis of the DA1 vPN morphogenesis, we should provide insights into future comprehension of how vPNs are assembled into the olfactory neural circuitry. PMID:27163287

  5. Low strain, long life creep fatigue of AF2-1DA and INCO 718

    SciTech Connect

    Thakker, A.B.; Cowles, B.A.

    1983-04-01

    Two aircraft turbine disk alloys, GATORIZED AF2-DA and INCO 718 were evaluated for their low strain long life creep-fatigue behavior. Static (tensile and creep rupture) and cyclic properties of both alloys were characterized. The cntrolled strain LCF tests were conducted at 760 C (1400 F) and 649 C (1200 F) for AF2-1DA and INCO 718, respectively. Hold times were varied for tensile, compressive and tensile/compressive strain dwell (relaxation) tests. Stress (creep) hold behavior of AF2-1DA was also evaluated. Generally, INCO 718 exhibited more pronounced reduction in cyclic life due to hold than AF2-1DA. The percent reduction in life for both alloys for strain dwell tests was greater at low strain ranges (longer life regime). Changing hold time from 0 to 0.5, 2.0 and 15.0 min. resulted in corresponding reductions in life. The continuous cycle and cyclic/dwell initiation failure mechanism was predominantly transgranular for AF2-1DA and intergranular for INCO 718.

  6. PanDA: Exascale Federation of Resources for the ATLAS Experiment at the LHC

    NASA Astrophysics Data System (ADS)

    Barreiro Megino, Fernando; Caballero Bejar, Jose; De, Kaushik; Hover, John; Klimentov, Alexei; Maeno, Tadashi; Nilsson, Paul; Oleynik, Danila; Padolski, Siarhei; Panitkin, Sergey; Petrosyan, Artem; Wenaus, Torre

    2016-02-01

    After a scheduled maintenance and upgrade period, the world's largest and most powerful machine - the Large Hadron Collider(LHC) - is about to enter its second run at unprecedented energies. In order to exploit the scientific potential of the machine, the experiments at the LHC face computational challenges with enormous data volumes that need to be analysed by thousand of physics users and compared to simulated data. Given diverse funding constraints, the computational resources for the LHC have been deployed in a worldwide mesh of data centres, connected to each other through Grid technologies. The PanDA (Production and Distributed Analysis) system was developed in 2005 for the ATLAS experiment on top of this heterogeneous infrastructure to seamlessly integrate the computational resources and give the users the feeling of a unique system. Since its origins, PanDA has evolved together with upcoming computing paradigms in and outside HEP, such as changes in the networking model, Cloud Computing and HPC. It is currently running steadily up to 200 thousand simultaneous cores (limited by the available resources for ATLAS), up to two million aggregated jobs per day and processes over an exabyte of data per year. The success of PanDA in ATLAS is triggering the widespread adoption and testing by other experiments. In this contribution we will give an overview of the PanDA components and focus on the new features and upcoming challenges that are relevant to the next decade of distributed computing workload management using PanDA.

  7. Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis

    PubMed Central

    Song, Hyun-Ouk

    2016-01-01

    Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis. PMID:26951982

  8. Experience with ATLAS MySQL PanDA database service

    NASA Astrophysics Data System (ADS)

    Smirnov, Y.; Wlodek, T.; De, K.; Hover, J.; Ozturk, N.; Smith, J.; Wenaus, T.; Yu, D.

    2010-04-01

    The PanDA distributed production and analysis system has been in production use for ATLAS data processing and analysis since late 2005 in the US, and globally throughout ATLAS since early 2008. Its core architecture is based on a set of stateless web services served by Apache and backed by a suite of MySQL databases that are the repository for all PanDA information: active and archival job queues, dataset and file catalogs, site configuration information, monitoring information, system control parameters, and so on. This database system is one of the most critical components of PanDA, and has successfully delivered the functional and scaling performance required by PanDA, currently operating at a scale of half a million jobs per week, with much growth still to come. In this paper we describe the design and implementation of the PanDA database system, its architecture of MySQL servers deployed at BNL and CERN, backup strategy and monitoring tools. The system has been developed, thoroughly tested, and brought to production to provide highly reliable, scalable, flexible and available database services for ATLAS Monte Carlo production, reconstruction and physics analysis.

  9. Synergistic effects of melatonin and deprenyl against MPTP-induced mitochondrial damage and DA depletion.

    PubMed

    Khaldy, Hoda; Escames, Germaine; León, Josefa; Bikjdaouene, Leila; Acuña-Castroviejo, Darío

    2003-01-01

    Previous studies showed a synergistic effect of melatonin and deprenyl against dopamine (DA) autoxidation in vitro. Since oxidative stress is implicated in Parkinson's disease (PD), we explored the effects of melatonin plus deprenyl administration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD in C57/Bl6 mice. Melatonin, but not deprenyl prevents the inhibition of mitochondrial complex I and the oxidative damage in nigrostriatal neurons induced by MPTP. With the dose used deprenyl recovers 50% DA levels and tyrosine hydroxylase activity depressed by the neurotoxin, normalizing locomotor activity of mice. Melatonin, which was unable to counteract MPTP-induced DA depletion and inhibition of tyrosine hydroxylase activity, potentiates the effect of deprenyl on catecholamine turnover and mice ambulatory activity. These results suggest a dissociation of complex I inhibition from DA depletion in this model of Parkinson's disease. The data also support that a combination of melatonin, which improves mitochondrial electron transport chain and reduces oxidative damage, and deprenyl, which promotes the specific function of the rescued neurons, i.e. DA turnover, may be a promising strategy for the treatment of PD. PMID:12600724

  10. Low strain, long life creep fatigue of AF2-1DA and INCO 718

    NASA Technical Reports Server (NTRS)

    Thakker, A. B.; Cowles, B. A.

    1983-01-01

    Two aircraft turbine disk alloys, GATORIZED AF2-DA and INCO 718 were evaluated for their low strain long life creep-fatigue behavior. Static (tensile and creep rupture) and cyclic properties of both alloys were characterized. The cntrolled strain LCF tests were conducted at 760 C (1400 F) and 649 C (1200 F) for AF2-1DA and INCO 718, respectively. Hold times were varied for tensile, compressive and tensile/compressive strain dwell (relaxation) tests. Stress (creep) hold behavior of AF2-1DA was also evaluated. Generally, INCO 718 exhibited more pronounced reduction in cyclic life due to hold than AF2-1DA. The percent reduction in life for both alloys for strain dwell tests was greater at low strain ranges (longer life regime). Changing hold time from 0 to 0.5, 2.0 and 15.0 min. resulted in corresponding reductions in life. The continuous cycle and cyclic/dwell initiation failure mechanism was predominantly transgranular for AF2-1DA and intergranular for INCO 718.

  11. Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase

    SciTech Connect

    Shoseyov, O.; Doi, R.H. )

    1990-03-01

    The cellulase complex from Clostridium cellulovorans has been purified and its subunit composition determined. The complex exhibits cellulase activity against crystalline cellulose as well as carboxymethylcellulase (CMCase) and cellobiohydrolase activities. Three major subunits are present with molecular masses of 170, 100, and 70 kDa. The 100-kDa subunit is the major CMCase, although at least four other, minor subunits show CMCase activity. The 170-kDa subunit has the highest affinity for cellulose, does not have detectable enzymatic activity, but is necessary for cellulase activity. Immunological studies indicate that the 170-kDa subunit is not required for binding of the catalytic subunits to cellulose and therefore does not function solely as an anchor protein. Thus this core subunit must have multiple functions. The authors propose a working hypothesis that the binding of the 170-kDa subunit converts the crystalline cellulose to a form that is capable of being hydrolyzed in a cooperative fashion by the associated catalytic subunits.

  12. Detection of trace helium in G104 - 27, a 26,000 K DA white dwarf

    NASA Technical Reports Server (NTRS)

    Holberg, J. B.; Kidder, K. M.; Wesemael, F.

    1990-01-01

    The detection of the He I 4471 A feature in the moderately hot DA white dwarf G104 - 27 places this star within the rather sparse class of DAB white dwarfs. It does not appear possible, on the basis of the present data, to distinguish between a stratified and a homogeneously mixed atmosphere. While a stratified structure would be natural for a DA white dwarf, G104 - 27 resides near the red edge of the DB gap, in a region where DA stars could transform into DB stars through convective mixing in the underlying He envelope and thereby destroy envelope stratification. Both atmospheres are consistent with the existing soft X-ray flux limit for this star.

  13. Fluorescence spectroscopy coupled with PARAFAC and PLS DA for characterization and classification of honey.

    PubMed

    Lenhardt, Lea; Bro, Rasmus; Zeković, Ivana; Dramićanin, Tatjana; Dramićanin, Miroslav D

    2015-05-15

    Fluorescence spectroscopy coupled with parallel factor analysis (PARAFAC) and Partial least squares Discriminant Analysis (PLS DA) were used for characterization and classification of honey. Excitation emission spectra were obtained for 95 honey samples of different botanical origin (acacia, sunflower, linden, meadow, and fake honey) by recording emission from 270 to 640 nm with excitation in the range of 240-500 nm. The number of fluorophores present in honey, excitation and emission spectra of each fluorophore, and their relative concentration are determined using a six-component PARAFAC model. Emissions from phenolic compounds and Maillard reaction products exhibited the largest difference among classes of honey of different botanical origin. The PLS DA classification model, constructed from PARAFAC model scores, detected fake honey samples with 100% sensitivity and specificity. Honey samples were also classified using PLS DA with errors of 0.5% for linden, 10% for acacia, and about 20% for both sunflower and meadow mix. PMID:25577082

  14. gLExec Integration with the ATLAS PanDA Workload Management System

    NASA Astrophysics Data System (ADS)

    Karavakis, E.; Barreiro, F.; Campana, S.; De, K.; Di Girolamo, A.; Litmaath, M.; Maeno, T.; Medrano, R.; Nilsson, P.; Wenaus, T.

    2015-12-01

    ATLAS user jobs are executed on Worker Nodes (WNs) by pilots sent to sites by pilot factories. This paradigm serves to allow a high job reliability and although it has clear advantages, such as making the working environment homogeneous, the approach presents security and traceability challenges. To address these challenges, gLExec can be used to let the payloads for each user be executed under a different UNIX user id that uniquely identifies the ATLAS user. This paper describes the recent improvements and evolution of the security model within the ATLAS PanDA system, including improvements in the PanDA pilot, in the PanDA server and their integration with MyProxy, a credential caching system that entitles a person or a service to act in the name of the issuer of the credential. Finally, it presents results from ATLAS user jobs running with gLExec and describes the deployment campaign within ATLAS.

  15. Chronic 5-HT transporter blockade reduces DA signaling to elicit basal ganglia dysfunction.

    PubMed

    Morelli, Emanuela; Moore, Holly; Rebello, Tahilia J; Gray, Neil; Steele, Kelly; Esposito, Ennio; Gingrich, Jay A; Ansorge, Mark S

    2011-11-01

    Serotonin (5-HT)-selective reuptake inhibitors (SSRIs) are widely administered for the treatment of depression, anxiety, and other neuropsychiatric disorders, but response rates are low, and side effects often lead to discontinuation. Side effect profiles suggest that SSRIs inhibit dopaminergic activity, but mechanistic insight remains scarce. Here we show that in mice, chronic 5-HT transporter (5-HTT) blockade during adulthood but not during development impairs basal ganglia-dependent behaviors in a dose-dependent and reversible fashion. Furthermore, chronic 5-HTT blockade reduces striatal dopamine (DA) content and metabolism. A causal relationship between reduced DA signaling and impaired basal ganglia-dependent behavior is indicated by the reversal of behavioral deficits through L-DOPA administration. Our data suggest that augmentation of DA signaling would reduce side effects and increase efficacies of SSRI-based therapy. PMID:22049417

  16. The Da Vinci European BioBank: A Metabolomics-Driven Infrastructure

    PubMed Central

    Carotenuto, Dario; Luchinat, Claudio; Marcon, Giordana; Rosato, Antonio; Turano, Paola

    2015-01-01

    We present here the organization of the recently-constituted da Vinci European BioBank (daVEB, https://www.davincieuropeanbiobank.org/it). The biobank was created as an infrastructure to support the activities of the Fiorgen Foundation (http://www.fiorgen.net/), a nonprofit organization that promotes research in the field of pharmacogenomics and personalized medicine. The way operating procedures concerning samples and data have been developed at daVEB largely stems from the strong metabolomics connotation of Fiorgen and from the involvement of the scientific collaborators of the foundation in international/European projects aimed to tackle the standardization of pre-analytical procedures and the promotion of data standards in metabolomics. PMID:25913579

  17. Evolution of the ATLAS PanDA Production and Distributed Analysis System

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Wenaus, T.; Nilsson, P.; Walker, R.; Stradling, A.; Fine, V.; Potekhin, M.; Panitkin, S.; Compostella, G.

    2012-12-01

    The PanDA (Production and Distributed Analysis) system has been developed to meet ATLAS production and analysis requirements for a data-driven workload management system capable of operating at LHC data processing scale. PanDA has performed well with high reliability and robustness during the two years of LHC data-taking, while being actively evolved to meet the rapidly changing requirements for analysis use cases. We will present an overview of system evolution including automatic rebrokerage and reattempt for analysis jobs, adaptation for the CernVM File System, support for the multi-cloud model through which Tier-2 sites act as members of multiple clouds, pledged resource management and preferential brokerage, and monitoring improvements. We will also describe results from the analysis of two years of PanDA usage statistics, current issues, and plans for the future.

  18. The coolest DA white dwarfs detected at soft X-ray wavelengths

    NASA Technical Reports Server (NTRS)

    Kidder, K. M.; Holberg, J. B.; Barstow, M. A.; Tweedy, R. W.; Wesemael, F.

    1992-01-01

    New soft X-ray/EUV photometric observations of the DA white dwarfs KPD 0631 + 1043 = WD 0631 + 107 and PG 1113 + 413 = WD 1113 + 413 are analyzed. Previously reported soft X-ray detections of three other DAs and the failure to detect a fourth DA in deep Exosat observations are investigated. New ground-based spectra are presented for all of the objects, with IUE Ly-alpha spectra for some. These data are used to constrain the effective temperatures and surface gravities. The improved estimates of these parameters are employed to refer a photospheric He abundance for the hotter objects and to elucidate an effective observational low-temperature threshold for the detection of pure hydrogen DA white dwarfs at soft X-ray wavelengths.

  19. Integration of PanDA workload management system with Titan supercomputer at OLCF

    NASA Astrophysics Data System (ADS)

    De, K.; Klimentov, A.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.

    2015-12-01

    The PanDA (Production and Distributed Analysis) workload management system (WMS) was developed to meet the scale and complexity of LHC distributed computing for the ATLAS experiment. While PanDA currently distributes jobs to more than 100,000 cores at well over 100 Grid sites, the future LHC data taking runs will require more resources than Grid computing can possibly provide. To alleviate these challenges, ATLAS is engaged in an ambitious program to expand the current computing model to include additional resources such as the opportunistic use of supercomputers. We will describe a project aimed at integration of PanDA WMS with Titan supercomputer at Oak Ridge Leadership Computing Facility (OLCF). The current approach utilizes a modified PanDA pilot framework for job submission to Titan's batch queues and local data management, with light-weight MPI wrappers to run single threaded workloads in parallel on Titan's multicore worker nodes. It also gives PanDA new capability to collect, in real time, information about unused worker nodes on Titan, which allows precise definition of the size and duration of jobs submitted to Titan according to available free resources. This capability significantly reduces PanDA job wait time while improving Titan's utilization efficiency. This implementation was tested with a variety of Monte-Carlo workloads on Titan and is being tested on several other supercomputing platforms. Notice: This manuscript has been authored, by employees of Brookhaven Science Associates, LLC under Contract No. DE-AC02-98CH10886 with the U.S. Department of Energy. The publisher by accepting the manuscript for publication acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes.

  20. Recurring X-ray outbursts in the supernova impostor SN 2010da in NGC 300

    NASA Astrophysics Data System (ADS)

    Binder, B.; Williams, B. F.; Kong, A. K. H.; Gaetz, T. J.; Plucinsky, P. P.; Skillman, E. D.; Dolphin, A.

    2016-04-01

    We present new observations of the `supernova impostor' SN 2010da using the Chandra X-ray Observatory and the Hubble Space Telescope. During the initial 2010 outburst, the 0.3-10 keV luminosity was observed by Swift to be ˜5 × 1038 erg s-1 and faded by a factor of ˜25 in a four month period. Our two new Chandra observations show a factor of ˜10 increase in the 0.35-8 keV X-ray luminosity, from ˜4 × 1036 to 4 × 1037 erg s-1 in ˜6 months, and the X-ray spectrum is consistent in both observations with a power-law with a photon index of Γ ˜ 0. We find evidence of X-ray spectral state changes: when SN 2010da is in a high-luminosity state, the X-ray spectrum is harder (Γ ˜0) compared to the low-luminosity state (Γ ˜ 1.2 ± 0.8). Using our Hubble observations, we fit the colour-magnitude diagram of the coeval stellar population to estimate a time since formation of the SN 2010da progenitor system of ≲5 Myr. Our observations are consistent with SN 2010da being a high-mass X-ray binary (HMXB) composed of a neutron star and a luminous blue variable-like companion, although we cannot rule out the possibility that SN 2010da is an unusually X-ray bright massive star. The ≲5 Myr age is consistent with the theoretically predicted delay time between the formation of a massive binary and the onset of the HMXB phase. It is possible that the initial 2010 outburst marked the beginning of X-ray production in the system, making SN 2010da possibly the first massive progenitor binary ever observed to evolve into an HMXB.

  1. Characterization of the 70 kDa polypeptide of the Na/Ca exchanger.

    PubMed Central

    Saba, R I; Bollen, A; Herchuelz, A

    1999-01-01

    The Na/Ca exchanger is associated with 160, 120 and 70 kDa polypeptides whose nature is poorly understood. We have purified and characterized the Na/Ca exchanger from bovine cardiac sarcolemmal vesicles (SLVs) by using ion-exchange and affinity chromatographies. The Na/Ca exchanger-enriched fraction was reconstituted into asolectin liposomes [lipid to protein ratio 10:1 (w/w)] that showed Na/Ca exchange activity. Under non-reducing conditions, SDS/PAGE showed a single 70 kDa polypeptide, which was further characterized by immunoblots with different antibodies: SWant, raised against the purified exchanger protein; NH2-terminus, residues 1-21; NCX1, residues 393-406; and Exon F, residues 622-644. Immunoblots under reducing conditions with SWant, NH2-terminus and NCX1 showed three bands migrating at 160, 120 and 70 kDa for SLV preparations, whereas Exon F reacted only with the 160 and 120 kDa bands. Under non-reducing conditions, immunoblots with purified reconstituted Na/Ca exchanger showed a single band at 70 kDa reacting with SWant, NH2-terminus and NCX1 but not with Exon F. We conclude that the 70 kDa protein is associated with Na/Ca exchange activity, has the same N-terminal sequence as the cloned bovine cardiac exchanger, and has its length decreased by at least 35% from its C-terminal portion as compared with that of the wild-type exchanger. PMID:9931309

  2. Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil

    PubMed Central

    Song, Ok-Ryul; Lee, Seung-Jin; Lee, Yong-Seok; Lee, Sang-Cheol; Kim, Keun-Ki; Choi, Yong-Lark

    2008-01-01

    A mineral phosphate solubilizing bacterium, Burkholderia cepacia DA23 has been isolated from cultivated soils. Phosphate-solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. When 3% of glucose concentration was used for carbon source, the strain had a marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was directly related to the pH drop by the strain. Analysis of the culture medium by high pressure liquid chromatography identified gluconic acid as the main organic acid released by Burkholderia cepacia DA23. Gluconic acid production was apparently the result of the glucose dehydrogenase activity and glucose dehydrogenase was affected by phosphate regulation. PMID:24031195

  3. Spectroscopy of the DA white dwarfs - Automatic atmospheric parameterization and mass distribution

    NASA Technical Reports Server (NTRS)

    Mcmahan, Robert K.

    1989-01-01

    A method for the automatic calculation of the atmospheric parameters (Teff and log g) of hydrogen-rich degenerate stars from low-resolution spectra is described, and then applied to the spectra of 53 DA white dwarfs. A value for the width of the DA mass distribution of sigma M/solar-M not greater than +0.10 is obtained using the proposed approach. The data indicate that the distribution is asymmetrically skewed to low masses; however, there is also evidence of a high-mass non-Gaussian tail.

  4. [Euclides da Cunha in the Amazon: historical discontinuities in how the forest is seen and narrated].

    PubMed

    Guimarães, Leandro Belinaso

    2010-01-01

    The article examines how the discursive, social, and political processes that nationalized the Amazon gained firm footing during Brazil's First Republic. It is argued that Euclides da Cunha's texts on the Amazon see the forest and narrate its story in a way that differs from the travel accounts of nineteenth-century naturalists, above all foreign ones. Drawing inspiration from Latin American cultural studies, the article reinforces the argument that there is a historical discontinuity between the romantic foreign travel literature of the nineteenth century and the early twentieth-century writings of Euclides da Cunha. PMID:21461535

  5. Aerodynamic design of the Cal Poly Da Vinci Human-Powered Helicopter

    NASA Technical Reports Server (NTRS)

    Larwood, Scott; Saiki, Neal

    1990-01-01

    This paper will discuss the methodology used in designing the rotor and drive propellers for the third generation Cal Poly Da Vinci Human-Powered Helicopter. The rotor was designed using a lifting surface, uniform inflow hover analysis code and the propeller was designed using a minimum induced-loss method. Construction, geometry, and operating considerations are discussed as they impact the designs. Optimization of the design performance is also explained. The propellers were tested in a wind tunnel and results are compared with theoretical data. Successful flight tests of the Da Vinci III are discussed.

  6. The prototypical 4.1R-10-kDa domain and the 4.1g-10-kDa paralog mediate fodrin-actin complex formation.

    PubMed

    Kontrogianni-Konstantopoulos, A; Frye, C S; Benz, E J; Huang, S C

    2001-06-01

    A complex family of 4.1R isoforms has been identified in non-erythroid tissues. In this study we characterized the exonic composition of brain 4.1R-10-kDa or spectrin/actin binding (SAB) domain and identified the minimal sequences required to stimulate fodrin/F-actin association. Adult rat brain expresses predominantly 4.1R mRNAs that carry an extended SAB, consisting of the alternative exons 14/15/16 and part of the constitutive exon 17. Exon 16 along with sequences carried by exon 17 is necessary and sufficient to induce formation of fodrin-actin-4.1R ternary complexes. The ability of the respective SAB domains of 4.1 homologs to sediment fodrin/actin was also investigated. 4.1G-SAB stimulates association of fodrin/actin, although with an approximately 2-fold reduced efficiency compared with 4.1R-10-kDa, whereas 4.1N and 4.1B do not. Sequencing of the corresponding domains revealed that 4.1G-SAB carries a cassette that shares significant homology with 4.1R exon 16, whereas the respective sequence is divergent in 4.1N and absent from brain 4.1B. An approximately 150-kDa 4.1R and an approximately 160-kDa 4.1G isoforms are present in PC12 lysates that occur in vivo in a supramolecular complex with fodrin and F-actin. Moreover, proteins 4.1R and 4.1G are distributed underneath the plasma membrane in PC12 cells. Collectively, these observations suggest that brain 4.1R and 4.1G may modulate the membrane mechanical properties of neuronal cells by promoting fodrin/actin association. PMID:11274145

  7. Análise da aplicação e dos resultados do modelo OPM3® para a área da saúde

    PubMed Central

    Augusto dos Santos, Luis; de Fátima Marin, Heimar

    2015-01-01

    Esta pesquisa procurou analisar se um modelo de questionário criado por uma comunidade internacional de gerenciamento de projetos e se é aplicavél a organizações voltadas a área de saúde. O modelo OPM3® (Organizational Project Management Maturity Model) foi criado para que organizações de qualquer área ou porte pudessem identificar a presença, ou ausência, de boas práticas de gerenciamento. O objetivo da aplicação desse modelo é avaliar sempre a organização e não o entrevistado. No presente artigo, são apresentados os resultados da aplicação desse modelo em uma organização que possuía produtos e serviços de tecnologia da informação aplicados à área de saúde. Este estudo verificou que o modelo é aplicável de forma rápida e que a organização analisada possuía um número expressivo de boas práticas. PMID:26924862

  8. Detection and differentiation of 22 kDa and 20 kDa Growth Hormone proteoforms in human plasma by LC-MS/MS.

    PubMed

    Such-Sanmartín, Gerard; Bache, Nicolai; Bosch, Jaume; Gutiérrez-Gallego, Ricardo; Segura, Jordi; Jensen, Ole N

    2015-04-01

    Human growth hormone (GH) is suspected to be widely and illegally used in sport to improve athletes' performance. For the detection of GH abuse, blood samples are screened for abnormal ratios between the 22 and 20 kDa GH proteoforms that demonstrate the administration of the synthetic hormone. Current detection methods are based on classical immunoassays as they provide sufficient sensitivity for the detection of GH proteoforms. These antibody based methods, however, suffer from unclear selectivity and potential cross-reactivity towards similar proteins. For unambiguous GH detection, we report a Mass Spectrometry ImmunoAssay (MSIA) that first enriches GH from plasma with an antibody of relatively low specificity, and subsequently quantifies the 22 and 20 kDa proteoforms by Selected Reaction Monitoring (SRM) LC-MS/MS analysis. This method proved superior to an antibody-free strategy based on GH purification by protein precipitation. Using GH-MSIA we successfully quantified the 22/20 kDa GH ratio in post-exercise capillary plasma extracted from two individuals. This GH-MSIA is applicable to anti-doping and GH-related disease analysis. PMID:25556001

  9. 76 FR 56455 - DaVita, Inc.; Analysis of Agreement Containing Consent Orders To Aid Public Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-13

    ... terms of the Consent Agreement, DaVita is required to divest 28 dialysis clinics and terminate one... Commission Act, as amended, 15 U.S.C. 45, by lessening competition for the provision of outpatient dialysis... provider of outpatient dialysis services in the United States. DaVita operates 1,612 outpatient...

  10. The 37/67kDa laminin receptor (LR) inhibitor, NSC47924, affects 37/67kDa LR cell surface localization and interaction with the cellular prion protein

    PubMed Central

    Sarnataro, Daniela; Pepe, Anna; Altamura, Gennaro; De Simone, Imma; Pesapane, Ada; Nitsch, Lucio; Montuori, Nunzia; Lavecchia, Antonio; Zurzolo, Chiara

    2016-01-01

    The 37/67 kDa laminin receptor (LR) is a non-integrin protein, which binds both laminin-1 of the extracellular matrix and prion proteins, that hold a central role in prion diseases. The 37/67 kDa LR has been identified as interactor for the prion protein (PrPC) and to be required for pathological PrP (PrPSc) propagation in scrapie-infected neuronal cells, leading to the possibility that 37/67 kDa LR-PrPC interaction is related to the pathogenesis of prion diseases. A relationship between 37/67 kDa LR and PrPC in the presence of specific LR inhibitor compounds has not been investigated yet. We have characterized the trafficking of 37/67 kDa LR in both neuronal and non-neuronal cells, finding the receptor on the cell surface and nuclei, and identified the 67 kDa LR as the almost exclusive isoform interacting with PrPC. Here, we show that the treatment with the 37/67 kDa LR inhibitor, NSC47924, affects both the direct 37/67 kDa LR-PrPC interaction in vitro and the formation of the immunocomplex in live cells, inducing a progressive internalization of 37/67 kDa LR and stabilization of PrPC on the cell surface. These data reveal NSC47924 as a useful tool to regulate PrPC and 37/67 kDa LR trafficking and degradation, representing a novel small molecule to be tested against prion diseases. PMID:27071549

  11. Effect of DA-9701 on the Normal Motility and Clonidine-induced Hypomotility of the Gastric Antrum in Rats

    PubMed Central

    Kang, Je Wook; Han, Dae Kyeong; Kim, Ock Nyun; Lee, Kwang Jae

    2016-01-01

    Background/Aims DA-9701 is a novel prokinetic agent. In the present study, we investigated the effect of DA-9701 on the motility of the gastric antrum in the normal and clonidine-induced hypomotility in an in vivo animal model. Methods A strain gauge force transducer was sutured on the gastric antrum to measure the contractile activity in rats. A total of 28 rats were subclassified into the 4 groups: (1) the placebo group, (2) the DA-9701 group, (3) the placebo group in the clonidine-pretreated rats, and (4) the DA-9701 group in the clonidine-pretreated rats. After the basal recording, either placebo (3% [w/v] hydroxypropylmethyl cellulose) or DA-9701 was administered. Contractile signals were measured after the administration and after a meal. In the clonidine-pretreated rats, either placebo or DA-9701 was administered. Contractile signals were measured after the administration and after a meal. Results Oral administration of DA-9701 did not significantly alter the motility index of the gastric antrum in the preprandial and postprandial periods, compared with the placebo group. The administration of clonidine decreased the motility index of the gastric antrum in the preprandial and postprandial periods, compared with the administration of placebo. This reduction of the antral motility by the administration of clonidine was not observed in the clonidine-pretreated DA-9701 group. The percentage of the motility index in the postprandial period was significantly greater in the clonidine-pretreated DA-9701 group, compared with the clonidine-pretreated placebo group. Conclusions DA-9701 improves the hypomotility of the gastric antrum induced by clonidine, suggesting its gastroprokinetic effect in the pathologic condition. PMID:26755679

  12. 32 CFR 516.14 - Service of process on DA or Secretary of Army.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Service of process on DA or Secretary of Army. 516.14 Section 516.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Service of Process § 516.14 Service of process on...

  13. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 4 2011-07-01 2011-07-01 false DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  14. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 4 2013-07-01 2013-07-01 false DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  15. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 4 2012-07-01 2011-07-01 true DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  16. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  17. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 4 2014-07-01 2013-07-01 true DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  18. Purification and characterization of the acid soluble 26-kDa polypeptide from soybean seeds.

    PubMed

    Momma, M; Haraguchi, K; Saito, M; Chikuni, K; Harada, K

    1997-08-01

    Whey proteins from soybean seeds of Japanese varieties were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Among 11 varieties of soybean, three green and one black soybeans lacked a 26-kDa band that was found in all yellow soybeans. In this paper, the 26-kDa protein was named AS26k (acid soluble 26-kDa protein) temporarily. The AS26k protein was purified from Glycine max cv. Nattosyoryu, which is yellow soybean, through four purification steps: 30-35% saturated ammonium sulfate fractionation, ion exchange chromatography on S Sepharose Fast Flow, gel filtration on Sephadex G-100, and hydrophobic chromatography on phenyl Sepharose CL-4B. Purified AS26k was cleaved with V8 proteinase from Staphylococcus aureus or CNBr. The cleaved polypeptide contained two typical dehydrin motif sequences: DEYGNPV and (M)DKIKEKLPG, and a 19 amino acids sequence similar to a pea dehydrin. Native AS26k had a molecular mass of 32 kDa on gel filtration and a pl of 7.2 on two-dimensional PAGE. Similarly to other dehydrins and late embryogenesis abundant (LEA) proteins, AS26k was rich in hydrophilic amino acids, and highly heat stable. These results showed that AS26k was a dehydrin, a group II LEA protein in soybean seeds. PMID:9301109

  19. Assessments of the economic impacts of natural hazards in Albania: application of DaLA methodology.

    NASA Astrophysics Data System (ADS)

    Massabo, Marco; Toto, Emanuela; Deda, Miranda; Prenci, Shemsi; Dhima, Maksimiljan

    2015-04-01

    Disaster loss datasets are built using economic assessments done by different parties and using different approaches which are in many cases inconsistent. This is observed in more than 75% of the Alabanian records with missing economic evaluations. To assess the economic impact of disasters at all scales in Global Assessment Report on Disaster Risk Reduction 2011 and 2013 has used the DaLA methodology that takes advantage of a common and homogeneous set of quantitative physical damage indicators contained in the disaster databases. These have to be taken as the lower bound of the damage, which in general is higher. The UN-ECLAC DaLA Methodology takes into account in its assessments the overall status of economy of the affected country. It uses the national accounts and statistics of the country government as baseline data to assess damage and loss. It also factors in the impact of disasters on individual livelihoods and incomes to fully define the needs for recovery and reconstruction. Similarly to national disaster loss databases DaLA assessments have a national level of observation and data produced at sub-national resolution, resulting in highly detailed and accurate data sets. In 2013 in Albania was implemented a collection of disaster losses based on Desinventar. We applied the DaLa methodology to the Albanian database of impacts of natural disasters.

  20. 40 CFR 60.40Da - Applicability and designation of affected facility.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... combination with any other fuel) in the combustion turbine engine and associated heat recovery steam generator... Performance for Electric Utility Steam Generating Units § 60.40Da Applicability and designation of affected... subpart applies is each electric utility steam generating unit: (1) That is capable of combusting...

  1. 40 CFR 60.40Da - Applicability and designation of affected facility.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... combination with any other fuel) in the combustion turbine engine and associated heat recovery steam generator... Performance for Electric Utility Steam Generating Units § 60.40Da Applicability and designation of affected... subpart applies is each electric utility steam generating unit: (1) That is capable of combusting...

  2. 40 CFR 60.40Da - Applicability and designation of affected facility.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... combination with any other fuel) in the combustion turbine engine and associated heat recovery steam generator... Performance for Electric Utility Steam Generating Units § 60.40Da Applicability and designation of affected... subpart applies is each electric utility steam generating unit: (1) That is capable of combusting...

  3. Long-term evolution of 1991 DA: A dynamically evolved extinct Halley-type comet

    NASA Technical Reports Server (NTRS)

    Hahn, Gerhard; Bailey, M. E.

    1992-01-01

    The long-term dynamical evolution of 21 variational orbits for the intermediate-period asteroid 1991 DA was followed for up to +/-10(exp 5) years from the present. 1991 DA is close to the 2:7 resonance with Jupiter; it has avoided close encounters, within 1 AU, with this planet for at least the past 30,000 years, even at the node crossing. The future evolution typically shows no close encounters with Jupiter within at least 50,000 years. This corresponds to the mean time between node crossings with either Jupiter or Saturn. Close encounters with Saturn and Jupiter lead to a chaotic evolution for the whole ensemble, while secular perturbations cause large-amplitude swings in eccentricity and inclination (the latter covering the range 15 deg approximately less than i approximately less than 85 deg) which correlate with deep excursions of the perihelion distance to values much less than 1 AU. These variations are similar to those found in P/Machholz and a variety of other high-inclination orbits, e.g., P/Hartley-IRAS. We emphasize the connection between the orbital evolution of 1991 DA and that of Halley-type comets. If 1991 DA was once a comet, it is not surprising that it is now extinct.

  4. Computerized Dynamic Assessment (C-DA): Diagnosing L2 Development According to Learner Responsiveness to Mediation

    ERIC Educational Resources Information Center

    Poehner, Matthew E.; Zhang, Jie; Lu, Xiaofei

    2015-01-01

    Dynamic assessment (DA) derives from the sociocultural theory of mind as elaborated by Russian psychologist L. S. Vygotsky. By offering mediation when individuals experience difficulties and carefully tracing their responsiveness, Vygotsky (1998) proposed that diagnoses may uncover abilities that have fully formed as well as those still in the…

  5. GS32, a novel Golgi SNARE of 32 kDa, interacts preferentially with syntaxin 6.

    PubMed

    Wong, S H; Xu, Y; Zhang, T; Griffiths, G; Lowe, S L; Subramaniam, V N; Seow, K T; Hong, W

    1999-01-01

    Syntaxin 1, synaptobrevins or vesicle-associated membrane proteins, and the synaptosome-associated protein of 25 kDa (SNAP-25) are key molecules involved in the docking and fusion of synaptic vesicles with the presynaptic membrane. We report here the molecular, cell biological, and biochemical characterization of a 32-kDa protein homologous to both SNAP-25 (20% amino acid sequence identity) and the recently identified SNAP-23 (19% amino acid sequence identity). Northern blot analysis shows that the mRNA for this protein is widely expressed. Polyclonal antibodies against this protein detect a 32-kDa protein present in both cytosol and membrane fractions. The membrane-bound form of this protein is revealed to be primarily localized to the Golgi apparatus by indirect immunofluorescence microscopy, a finding that is further established by electron microscopy immunogold labeling showing that this protein is present in tubular-vesicular structures of the Golgi apparatus. Biochemical characterizations establish that this protein behaves like a SNAP receptor and is thus named Golgi SNARE of 32 kDa (GS32). GS32 in the Golgi extract is preferentially retained by the immobilized GST-syntaxin 6 fusion protein. The coimmunoprecipitation of syntaxin 6 but not syntaxin 5 or GS28 from the Golgi extract by antibodies against GS32 further sustains the preferential interaction of GS32 with Golgi syntaxin 6. PMID:9880331

  6. Tumour-infiltrating lymphocytes bear the 75 kDa tumour necrosis factor receptor.

    PubMed Central

    Trentin, L.; Zambello, R.; Bulian, P.; Cerutti, A.; Enthammer, C.; Cassatella, M.; Nitti, D.; Lise, M.; Agostini, C.; Semenzato, G.

    1995-01-01

    Tumour necrosis factor alpha (TNF-alpha) is a cytokine with a variety of immunological properties. The identification of two receptors for this molecule, i.e. the 75 kDa and the 55 kDa TNF receptors (TNF-R), recently clarified the mechanisms through which this cytokine provides its wide range of immunomodulatory activities. In this study we have investigated the expression and the functional properties of these receptors on tumour-infiltrating lymphocytes (TILs) recovered from 17 patients with solid cancers (melanoma, colorectal carcinoma and lung cancer). To this end, TIL lines and freshly isolated TILs were evaluated for (a) the expression and the functional role of TNF receptors following culture in the presence of interleukin 2 (IL-2) and (b) the production of TNF-alpha following culture with IL-2 and the role of this cytokine in IL-2-driven TIL proliferation. Flow cytometry analysis demonstrated that TILs bear the 75 kDa TNF-R. Moreover, TIL lines express detectable messages for TNF-alpha and release this cytokine. Functional in vitro studies have shown that anti-TNF-alpha, as well as anti-75 kDa TNF-R antibodies, are able to inhibit the IL-2-induced TIL proliferation. These data demonstrate that TILs are equipped with a fully functional TNF-R system and suggest a putative role for this receptor and its ligand in the activation and expression of TILs following immunotherapy with IL-2. Images Figure 2 PMID:7841036

  7. Translocation of an 89-kDa periplasmic protein is associated with Holospora infection

    SciTech Connect

    Iwatani, Koichi; Dohra, Hideo; Lang, B. Franz; Burger, Gertraud; Hori, Manabu; Fujishima, Masahiro . E-mail: fujishim@yamaguchi-u.ac.jp

    2005-12-02

    The symbiotic bacterium Holospora obtusa infects the macronucleus of the ciliate Paramecium caudatum. After ingestion by its host, an infectious form of Holospora with an electron-translucent tip passes through the host digestive vacuole and penetrates the macronuclear envelope with this tip. To investigate the underlying molecular mechanism of this process, we raised a monoclonal antibody against the tip-specific 89-kDa protein, sequenced this partially, and identified the corresponding complete gene. The deduced protein sequence carries two actin-binding motifs. Indirect immunofluorescence microscopy shows that during escape from the host digestive vacuole, the 89-kDa proteins translocates from the inside to the outside of the tip. When the bacterium invades the macronucleus, the 89-kDa protein is left behind at the entry point of the nuclear envelope. Transmission electron microscopy shows the formation of fine fibrous structures that co-localize with the antibody-labeled regions of the bacterium. Our findings suggest that the 89-kDa protein plays a role in Holospora's escape from the host digestive vacuole, the migration through the host cytoplasm, and the invasion into the macronucleus.

  8. 40 CFR 60.50Da - Compliance determination procedures and methods.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Electric Utility Steam Generating Units § 60.50Da Compliance determination procedures and methods. (a) In... operator of an electric utility combined cycle gas turbine that does not meet the definition of an IGCC... of this part. The SO2 and NOX emission rates calculations from the gas turbine used in Method 19...

  9. 40 CFR 60.50Da - Compliance determination procedures and methods.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Electric Utility Steam Generating Units § 60.50Da Compliance determination procedures and methods. (a) In... calculations from the gas turbine used in Method 19 of appendix A-7 of this part are determined when the gas turbine is performance tested under subpart GG of this part. The potential uncontrolled PM emission...

  10. 40 CFR 60.50Da - Compliance determination procedures and methods.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Electric Utility Steam Generating Units § 60.50Da Compliance determination procedures and methods. (a) In... operator of an electric utility combined cycle gas turbine that does not meet the definition of an IGCC... of this part. The SO2 and NOX emission rates calculations from the gas turbine used in Method 19...

  11. A Integracao de Ensino das Ciencias da Saude (An Integrated Medical Education Program [in Brazil]).

    ERIC Educational Resources Information Center

    Pourchet-Campos, M. A.; Guimaraes Junior, Paulino

    At the Sixth Annual Reunion of the Brazilian Association of Medical Schools (VI Reuniao Anual da Associacao Brasileira de Escolas Medicas) leaders in the Brazilian medical profession proposed an integrated educational program for training students in the fields of medicine and public health. Under Brazil's present system of education, all…

  12. 40 CFR 60.44Da - Standards for nitrogen oxides (NOX).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Standards for nitrogen oxides (NOX). 60... Steam Generating Units § 60.44Da Standards for nitrogen oxides (NOX). (a) Except as provided in....20 Liquid fuels: Coal-derived fuels 210 0.50 Shale oil 210 0.50 All other fuels 130 0.30 Solid...

  13. 40 CFR 60.44Da - Standards for nitrogen oxides (NOX).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Standards for nitrogen oxides (NOX). 60... Steam Generating Units § 60.44Da Standards for nitrogen oxides (NOX). (a) Except as provided in....20 Liquid fuels: Coal-derived fuels 210 0.50 Shale oil 210 0.50 All other fuels 130 0.30 Solid...

  14. 40 CFR 60.44Da - Standards for nitrogen oxides (NOX).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Standards for nitrogen oxides (NOX). 60... Steam Generating Units § 60.44Da Standards for nitrogen oxides (NOX). (a) Except as provided in....20 Liquid fuels: Coal-derived fuels 210 0.50 Shale oil 210 0.50 All other fuels 130 0.30 Solid...

  15. 32 CFR 635.23 - DA Form 4833 (Commander's Report of Disciplinary or Administrative Action).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true DA Form 4833 (Commander's Report of Disciplinary or Administrative Action). 635.23 Section 635.23 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS LAW ENFORCEMENT...

  16. Brominated and radioiodinated derivatives of methylphenidate (MP): Potential imaging agents for the dopamine (DA) transporter

    SciTech Connect

    Pan, D.; Gatley, S.J.; Dewey, S.L.

    1994-05-01

    MP (Ritalin) is a psychomotor stimulant used in the treatment of attention-deficit hyperactivity disorder. The therapeutic properties of MP are thought to be mediated by its binding to a site on the DA transporter, resulting in inhibition of DA reuptake and enhanced levels of synaptic dopamine. MP also inhibits reuptake of norepinephrine (NE) in vitro. MP has two chiral centers, but its pharmacological activity is believed due solely to the d-threo isomer. We have found that d,l-threo-C-11 MP has favorable properties for PET studies, and therefore examined the effects of incorporating halogen atoms into the phenyl ring of MP, with a view to preparing C-11 and I-123 MP analogs as potential PET/SPECT tracers. We synthesized the 2-, 3- and 4-bromo MP analogs from the corresponding bromophenylacetonitriles by modification of the original synthesis of MP. In in vitro binding assays all three d,l-threo bromo compounds had higher affinities than MP for DA transporter sites labeled with tritiated WIN 35,428 (3->4-, 2->MP). They also showed high activity with NE reuptake sites labeled with tritiated nisoxetine. They were active in vivo as demonstrated by inhibition of heart uptake of tritiated NE in the mouse, and elevation of striatal extracellular DA (microdialysis) and stimulation of locomotor activity in the rat.

  17. Next Generation PanDA Pilot for ATLAS and Other Experiments

    NASA Astrophysics Data System (ADS)

    Nilsson, P.; Barreiro Megino, F.; Caballero Bejar, J.; De, K.; Hover, J.; Love, P.; Maeno, T.; Medrano Llamas, R.; Walker, R.; Wenaus, T.; Atlas Collaboration

    2014-06-01

    The Production and Distributed Analysis system (PanDA) has been in use in the ATLAS Experiment since 2005. It uses a sophisticated pilot system to execute submitted jobs on the worker nodes. While originally designed for ATLAS, the PanDA Pilot has recently been refactored to facilitate use outside of ATLAS. Experiments are now handled as plug-ins such that a new PanDA Pilot user only has to implement a set of prototyped methods in the plug-in classes, and provide a script that configures and runs the experiment-specific payload. We will give an overview of the Next Generation PanDA Pilot system and will present major features and recent improvements including live user payload debugging, data access via the Federated XRootD system, stage-out to alternative storage elements, support for the new ATLAS DDM system (Rucio), and an improved integration with glExec, as well as a description of the experiment-specific plug-in classes. The performance of the pilot system in processing LHC data on the OSG, LCG and Nordugrid infrastructures used by ATLAS will also be presented. We will describe plans for future development on the time scale of the next few years.

  18. 32 CFR 516.14 - Service of process on DA or Secretary of Army.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Service of process on DA or Secretary of Army. 516.14 Section 516.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Service of Process § 516.14 Service of process on...

  19. 76 FR 55293 - Special Conditions: Diamond Aircraft Industries, Model DA-40NG; Electronic Engine Control (EEC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ... cantilever low wing, T-tail airplane with the Austro Engine GmbH model E4 diesel engine and an increased..., Model DA-40NG; Electronic Engine Control (EEC) System AGENCY: Federal Aviation Administration (FAA), DOT... design feature(s) associated with an electronic engine control (EEC), also known as a Full...

  20. 76 FR 72087 - Special Conditions: Diamond Aircraft Industries, Model DA-40NG; Electronic Engine Control (EEC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-22

    .... A47CE to include the new model DA- 40NG with the Austro Engine GmbH model E4 Aircraft Diesel Engine (ADE...-tail airplane with the Austro Engine GmbH model E4 diesel engine and an increased maximum takeoff gross... Engine GmbH model E4 aircraft diesel engine. 1. Electronic Engine Control a. For electronic...

  1. Views and Voices of the Working Class: Aída and Rosario

    ERIC Educational Resources Information Center

    Pao, Maria T.

    2014-01-01

    In 2005, Spanish television audiences saw the debut of the nation's first spinoff, the sitcom "Aída." The show featured the tribulations of its title character and her working-class family in their struggle to "llegar a fin de mes." It seemed to promise a sensibility enacted in the US series "Roseanne," where…

  2. Understanding and Interpreting Japanese NP1 "wa" NP2 "da" Sentences: Mechanism and Contextual Factors

    ERIC Educational Resources Information Center

    Yoshida, Megumi

    2013-01-01

    This dissertation investigates the contextual factors that affect the understanding and interpretation of one Japanese topicalized construction, NP[subscript 1] wa NP[subscript 2] da sentences, by native speakers of Japanese. The construction allows two possibilities in the relation between the NP[subscript 1] and the NP[subscript 2]. When the two…

  3. In response to Dr. Jose AP da Silva: fall prevention with vitamin D clarifications needed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We agree with Dr. da Silva, and wish to clarify three issues raised by the recent IOM report concerning our 2009 meta-analysis on vitamin D and fall prevention. 1. The IOM questioned the inclusion of Broe et al., which did not pre-specify falls as a primary or secondary outcome. While this did viol...

  4. 40 CFR 60.43Da - Standards for sulfur dioxide (SO2).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Standards for sulfur dioxide (SO2). 60... Steam Generating Units § 60.43Da Standards for sulfur dioxide (SO2). (a) On and after the date on which... the percent reduction requirement is determined on a 24-hour basis. (d) Sulfur dioxide emissions...

  5. 40 CFR 60.43Da - Standards for sulfur dioxide (SO2).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Standards for sulfur dioxide (SO2). 60... Steam Generating Units § 60.43Da Standards for sulfur dioxide (SO2). (a) On and after the date on which... the percent reduction requirement is determined on a 24-hour basis. (d) Sulfur dioxide emissions...

  6. 40 CFR 60.43Da - Standards for sulfur dioxide (SO2).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Standards for sulfur dioxide (SO2). 60... Steam Generating Units § 60.43Da Standards for sulfur dioxide (SO2). (a) On and after the date on which... the percent reduction requirement is determined on a 24-hour basis. (d) Sulfur dioxide emissions...

  7. MATERNAL ATRAZINE (ATR) ALTERS HYPOTHALAMIC DOPAMINE (HYP-DA) AND SERUM PROLACTIN (SPRL) IN MALE PUPS

    EPA Science Inventory

    Maternal Atrazine (ATR) alters hypothalamic dopamine (HYP-DA) and serum prolactin (sPRL) in male pups. 1Christopher Langdale, 2Tammy Stoker and 2Ralph Cooper. 1 Dept. of Cell Biology, North Carolina State University College of Veterinary Medicine, Raleigh, NC. 2 Endocrinology ...

  8. Leonardo da Vinci, One Year on...a Different Look at Vocational Training in Europe.

    ERIC Educational Resources Information Center

    Le Magazine, 1996

    1996-01-01

    Discusses the success of the Leonardo da Vinci program, a European laboratory of innovation in vocational training, a priority focus of investment in human resources and intelligence, and a way to mobilize innovative forces beyond national boundaries. Trends identified by the program focus on new information and communication technologies. (JOW)

  9. Transparency of Vocational Qualifications: The Leonardo da Vinci Approach. CEDEFOP Panorama Series.

    ERIC Educational Resources Information Center

    Bjornavold, Jens; Pettersson, Sten

    This report gives an overview of the situation of transparency of vocational qualifications by presenting measures introduced at the European Community level and by drawing attention to projects within the Leonardo da Vinci Program dealing with the issue. A 16-page executive summary appears first. Chapter 1 provides general background and aims.…

  10. Sobre a largura da última superfície de espalhamento

    NASA Astrophysics Data System (ADS)

    Nobre, M. A. S.; Pires, N.; Lima, J. A. S.

    2003-08-01

    De acordo com o modelo do "Big-Bang", no universo primordial a matéria estava em equilíbrio térmico com a radiação. Com a expansão a temperatura da radiação cai. Quando a temperatura chega em torno dos 4.000K, os espalhamentos diminuem, começando a recombinação dos prótons e elétrons em Hidrogênio neutro (era conhecida como da recombinação). Ao final da recombinação, os fótons se propagam livremente sofrendo, em princípio, somente os efeitos do "redshift" cosmológico. Esses fótons nos alcançam hoje como a radiação cósmica de fundo (RCF), e parecem vir de uma superfície esférica ao nosso redor, tal que o raio dela é a distância que ele viajou desde seu último espalhamento na época da recombinação. Naturalmente, esse processo não ocorreu abruptamente, implicando na existência de uma largura no espaço dos "redshifts" que deve depender do modelo cosmológico específico e dos processos físicos considerados. Neste trabalho analisamos os efeitos de diferentes modelos - a saber, aqueles com decaimento do vácuo L(t), criação de matéria, quintessência e gás de Chaplygin - sobre a última superfície de espalhamento da RCF, em particular sua largura e a função visibilidade, que determina a probabilidade de um fóton ter tido seu último espalhamento num "redshift" z. No caso particular dos modelos com decaimento do vácuo, existe uma forte dependência da função visibilidade com L(t). Tais efeitos poderão ser testados através da análise dos resultados de experimentos mais precisos que estão atualmente em andamento, como por exemplo, o WMAP.

  11. PanDA Pilot Submission using Condor-G: Experience and Improvements

    SciTech Connect

    Zhao X.; Hover John; Wlodek Tomasz; Wenaus Torre; Frey Jaime; Tannenbaum Todd; Livny Miron

    2011-01-01

    PanDA (Production and Distributed Analysis) is the workload management system of the ATLAS experiment, used to run managed production and user analysis jobs on the grid. As a late-binding, pilot-based system, the maintenance of a smooth and steady stream of pilot jobs to all grid sites is critical for PanDA operation. The ATLAS Computing Facility (ACF) at BNL, as the ATLAS Tier1 center in the US, operates the pilot submission systems for the US. This is done using the PanDA 'AutoPilot' scheduler component which submits pilot jobs via Condor-G, a grid job scheduling system developed at the University of Wisconsin-Madison. In this paper, we discuss the operation and performance of the Condor-G pilot submission at BNL, with emphasis on the challenges and issues encountered in the real grid production environment. With the close collaboration of Condor and PanDA teams, the scalability and stability of the overall system has been greatly improved over the last year. We review improvements made to Condor-G resulting from this collaboration, including isolation of site-based issues by running a separate Gridmanager for each remote site, introduction of the 'Nonessential' job attribute to allow Condor to optimize its behavior for the specific character of pilot jobs, better understanding and handling of the Gridmonitor process, as well as better scheduling in the PanDA pilot scheduler component. We will also cover the monitoring of the health of the system.

  12. PanDA Pilot Submission using Condor-G: Experience and Improvements

    NASA Astrophysics Data System (ADS)

    Zhao, Xin; Hover, John; Wlodek, Tomasz; Wenaus, Torre; Frey, Jaime; Tannenbaum, Todd; Livny, Miron; ATLAS Collaboration

    2011-12-01

    PanDA (Production and Distributed Analysis) is the workload management system of the ATLAS experiment, used to run managed production and user analysis jobs on the grid. As a late-binding, pilot-based system, the maintenance of a smooth and steady stream of pilot jobs to all grid sites is critical for PanDA operation. The ATLAS Computing Facility (ACF) at BNL, as the ATLAS Tier1 center in the US, operates the pilot submission systems for the US. This is done using the PanDA "AutoPilot" scheduler component which submits pilot jobs via Condor-G, a grid job scheduling system developed at the University of Wisconsin-Madison. In this paper, we discuss the operation and performance of the Condor-G pilot submission at BNL, with emphasis on the challenges and issues encountered in the real grid production environment. With the close collaboration of Condor and PanDA teams, the scalability and stability of the overall system has been greatly improved over the last year. We review improvements made to Condor-G resulting from this collaboration, including isolation of site-based issues by running a separate Gridmanager for each remote site, introduction of the 'Nonessential' job attribute to allow Condor to optimize its behavior for the specific character of pilot jobs, better understanding and handling of the Gridmonitor process, as well as better scheduling in the PanDA pilot scheduler component. We will also cover the monitoring of the health of the system.

  13. Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N terminus

    PubMed Central

    Havukainen, Heli; Halskau, Øyvind; Skjaerven, Lars; Smedal, Bente; Amdam, Gro V.

    2011-01-01

    Vitellogenin, an egg-yolk protein precursor common to oviparous animals, is found abundantly in honeybee workers – a caste of helpers that do not usually lay eggs. Instead, honeybee vitellogenin (180 kDa) participates in processes other than reproduction: it influences hormone signaling, food-related behavior, immunity, stress resistance and longevity. The molecular basis of these functions is largely unknown. Here, we establish and compare the molecular properties of vitellogenin from honeybee hemolymph (blood) and abdominal fat body, two compartments that are linked to vitellogenin functions. Our results reveal a novel 40 kDa vitellogenin fragment in abdominal fat body tissue, the main site for vitellogenin synthesis and storage. Using MALDI-TOF combined with MS/MS mass-spectroscopy, we assign the 40 kDa fragment to the N terminus of vitellogenin, whereas a previously observed 150 kDa fragment corresponded to the remainder of the protein. We show that both protein units are N glycosylated and phosphorylated. Focusing on the novel 40 kDa fragment, we present a homology model based on the structure of lamprey lipovitellin that includes a conserved β-barrel-like shape, with a lipophilic cavity in the interior and two insect-specific loops that have not been described before. Our data indicate that the honeybee fat body vitellogenin experiences cleavage unlike hemolymph vitellogenin, a pattern that can suggest a tissue-specific role. Our experiments advance the molecular understanding of vitellogenin, of which the multiple physiological and behavioral effects in honeybees are well established. PMID:21270306

  14. Site effects inferred from HVSR methodology in Praia da Vitória city (Portugal)

    NASA Astrophysics Data System (ADS)

    Fontiela, João; Borges, José; Bezzeghoud, Mourad; Rosset, Philippe; Rodrigues, Francisco

    2016-04-01

    Terceira island (Azores) have experienced several strong earthquakes, especially on the eastern and south-eastern part of the island with maximum MM intensity of VIII in 1800, 1801 and 1912 and IX in 1614 and 1841. Seismicity of the eastern part of the island is largely dominated by the tectonic of the NW-SE Lajes graben where are located Praia da Vitória and other setlements. Praia da Vitória is settled partially over pyroclastic material(pumice and ignimbrite) deposited around 20'000 years ago and forming part of the UpperTerceira Group. Its thickness is 15m on the north but in Praia da Vitoria has less than one meter.. It is sited on by basaltic flow of the upper Basaltic Terceira Group (2,000y BP) and a thick andesitic lava flow. A narrow band with sand dunes and beach crossed this deposits along Praia da Vitória and behind it appear a flat area with 7-10m of round cobbles that is interpreted as an old boulders'beach. Thickness of these different deposits are tricky to estimate in urban areas since very few outcrops are present. In order to assess site amplification of seismic waves due to the soil conditions, we perform horizontal-to-vertical spectral ratio (HVSR) method from broad band ambient noise measurements in 51 different sites within Praia da Vitoria. The first results show that the frequencies of the study area are variable, lying between 0.6-12 Hz. ACKNOWLEDGMENTS: João Fontiela is supported by grant M3.1.2/F/060/2011 provided by the Regional Science Fund of the Azores Regional Government. This study was partially funded by the Institute of Earth Sciences, Portugal, under the contract with the FCT, ICT-UID/GEO/04683/2013 (Portugal).

  15. Intestinal mucins from normal subjects and patients with cystic fibrosis. Variable contents of the disulphide-bound 118 kDa glycoprotein and different reactivities with an anti-(118 kDa glycoprotein) antibody.

    PubMed Central

    Mantle, M; Stewart, G

    1989-01-01

    1. A specific antibody was developed against the disulphide-bound 118 kDa glycoprotein of human intestinal mucin and used to establish an e.l.i.s.a. Fourteen purified mucins [eight normal (N) and six cystic fibrosis (CF)] had the same affinity for the antibody in the e.l.i.s.a., but their relative immunoreactivities varied widely (approx. 100,000-fold). In general, CF mucins were more antigenic than N mucins. 2. Variations (approx. 10-fold) were detected in the 118 kDa glycoprotein content of both N and CF mucins (assessed from Coomassie Blue-stained polyacrylamide gels), but these did not appear to be responsible for the differences in mucin immunoreactivity. 3. Variations (approx. 6-fold) were also observed in the size of the 118 kDa peak produced by N and CF mucins on Western blots. These were mostly due to differences in the 118 kDa glycoprotein content of mucins, although a small proportion resulted from changes in the number of antigenic determinants within individual 118 kDa glycoproteins. 4. After concanavalin A affinity chromatography of four reduced mucins (two N and two CF), purified 118 kDa glycoprotein was recovered in the bound fractions from the column, specifically eluted by methyl alpha-mannoside. 5. The amounts of 118 kDa glycoprotein isolated from the four mucins varied as predicted from the size of their 118 kDa bands on Coomassie Blue-stained gels. 6. Three 118 kDa glycoproteins (one N and two CF) showed almost identical reactivity in the e.l.i.s.a.; the fourth had fewer antigenic determinants. 7. Since differences in 118 kDa glycoprotein content and in the number of antigenic determinants within the 118 kDa glycoprotein did not account for variations in the reactivity of native mucins in the e.l.i.s.a., it appeared that accessibility of the 118 kDa glycoprotein to antibody binding may be critical in determining mucin immunoreactivity. This suggests that the three-dimensional conformation of CF mucins may differ from that of N mucins, leading

  16. Qual o Papel da Escola e da Mídia na Obtenção dos Conhecimentos Astronômicos?

    NASA Astrophysics Data System (ADS)

    da Cunha, W. S.; Voelzke, M. R.; Amaral, L. H.

    2007-08-01

    É amplamente reconhecido que as pessoas em geral têm grande fascínio e interesse pela Astronomia. Por outro lado, o conteúdo dessa área incluído no ensino formal está longe de ser abrangente e suficiente para suprir a demanda. Esse interesse permite aperfeiçoar a divulgação e o ensino não formal de Astronomia através da mídia aliando a adequação do conteúdo à expectativa das pessoas. A reforma do Currículo Básico da Escola Pública da maioria dos Estados brasileiros tem introduzido Astronomia desde a pré-escola até o Ensino Médio. Num estudo realizado com 1180 alunos do Ensino Médio de seis escolas estaduais, revelou-se através da aplicação de um formulário contendo questões que abordavam conhecimentos básicos em Astronomia, como foram adquiridos tais conhecimentos e à infra-estrutura da escola. Para 66,1% dos entrevistados o professor já fez alguma apresentação a respeito de Astronomia. O presente estudo revelou também que 54,4% dos alunos entrevistados adquiriram seus conhecimentos astronômicos na escola, 18,4% através da televisão e 27,1% afirmaram que seus conhecimentos foram obtidos através de outros tipos de mídia (internet, livros didáticos, filmes e revistas). Embora o estudo revele claramente que há certo equilíbrio no meio em que os alunos obtiveram seus conhecimentos astronômicos, a mídia busca não somente sua difusão, mas a disponibilização desse conhecimento de maneira correta, que às vezes, não é atingido, apresentando conceitos sem fundamentação e incorretos. Por outro lado, no dia-a-dia escolar, é comum encontrar professores que vêm para as aulas com concepções prévias, que podem diferir substancialmente das idéias a serem ensinadas, dificultando o aprendizado de futuros conceitos científicos. Uma avaliação criteriosa, avaliando as dificuldades dos professores em determinar o sentido correto dos conceitos e observando a metodologia que utilizam no ensino, poderá proporcionar uma melhor

  17. Dioscorea Extract (DA-9801) Modulates Markers of Peripheral Neuropathy in Type 2 Diabetic db/db Mice

    PubMed Central

    Moon, Eunjung; Lee, Sung Ok; Kang, Tong Ho; Kim, Hye Ju; Choi, Sang Zin; Son, Mi-Won; Kim, Sun Yeou

    2014-01-01

    The purpose of this study was to investigate the therapeutic effects of DA-9801, an optimized extract of Dioscorea species, on diabetic peripheral neuropathy in a type 2 diabetic animal model. In this study, db/db mice were treated with DA-9801 (30 and 100 mg/kg, daily, p.o.) for 12 weeks. DA-9801 reduced the blood glucose levels and increased the withdrawal latencies in hot plate tests. Moreover, it prevented nerve damage based on increased nerve conduction velocity and ultrastructural changes. Decrease of nerve growth factor (NGF) may have a detrimental effect on diabetic neuropathy. We previously reported NGF regulatory properties of the Dioscorea genus. In this study, DA-9801 induced NGF production in rat primary astrocytes. In addition, it increased NGF levels in the sciatic nerve and the plasma of type 2 diabetic animals. DA-9801 also increased neurite outgrowth and mRNA expression of Tieg1/Klf10, an NGF target gene, in PC12 cells. These results demonstrated the attenuation of diabetic peripheral neuropathy by oral treatment with DA-9801 via NGF regulation. DA-9801 is currently being evaluated in a phase II clinical study. PMID:25414776

  18. The Effect of DA-6034 on Intestinal Permeability in an Indomethacin-Induced Small Intestinal Injury Model

    PubMed Central

    Kwak, Dong Shin; Lee, Oh Young; Lee, Kang Nyeong; Jun, Dae Won; Lee, Hang Lak; Yoon, Byung Chul; Choi, Ho Soon

    2016-01-01

    Background/Aims DA-6034 has anti-inflammatory activities and exhibits cytoprotective effects in acute gastric injury models. However, explanations for the protective effects of DA-6034 on intestinal permeability are limited. This study sought to investigate the effect of DA-6034 on intestinal permeability in an indomethacin-induced small intestinal injury model and its protective effect against small intestinal injury. Methods Rats in the treatment group received DA-6034 from days 0 to 2 and indomethacin from days 1 to 2. Rats in the control group received indomethacin from days 1 to 2. On the fourth day, the small intestines were examined to compare the severity of inflammation. Intestinal permeability was evaluated by using fluorescein isothiocyanate-labeled dextran. Western blotting was performed to confirm the association between DA-6034 and the extracellular signal-regulated kinase (ERK) pathway. Results The inflammation scores in the treatment group were lower than those in the control group, but the difference was statistically insignificant. Hemorrhagic lesions in the treatment group were broader than those in the control group, but the difference was statistically insignificant. Intestinal permeability was lower in the treatment group than in the control group. DA-6034 enhanced extracellular signal-regulated kinase expression, and intestinal permeability was negatively correlated with ERK expression. Conclusions DA-6034 may decrease intestinal permeability in an indomethacin-induced intestinal injury model via the ERK pathway. PMID:27114435

  19. Lack of Apoptosis of Infiltrating Cells as the Mechanism of High Susceptibility to EAE in DA Rats

    PubMed Central

    Mensah-Brown, Eric; Galadari, Sehamuddin; Shahin, Allen

    2001-01-01

    Dark Agouti (DA) rats are highly susceptible to induction of Th-l-mediated autoimmunity disease, including experimental allergic encephalomyelitis (EAE). In contrast to other susceptible rat strains in which disease is induced only with encephalitogen emulsified in complete Freund's adjuvants (CFA), in DA rats EAE develops after injection of encephalitogen in incomplete Freund's adjuvants (IFA) or Titermax, putative Th-2 directed adjuvant. Lymph node cells derived from immunized DA rats and stimulated in vitro produce significantly more Interferon-γ (IFN-γ) than resistant Albino Oxford (AO) rats. However, cells derived from both strains produce large amounts of IL-10 but not IL-4. Immunized lymph node cells derived from EAE susceptible (AO × DA) F1rats induce clinical signs of disease in sublethally irradiated parental DA but not AO rats. The pathohistology of the target tissue in these recipients clearly demonstrated infiltration of mononuclear cells in both parental strains. However, the number of CD4+ cells was significantly higher and number of apoptotic cells significantly lower in DA rats sacrificed 8 days after passive transfer. We postulate that in addition to higher IFN-γ and TNF-α production, resistance to early apoptosis of the invading cells in the target tissue possibly due to lack of downregulation by TGF-β leads to exceptional susceptibility to EAE in DA rats. PMID:11785669

  20. Loss of 45 Da from a2 Ions and Preferential Loss of 48 Da from a2 Ions Containing Methionine in Peptide Ion Tandem Mass Spectra

    NASA Astrophysics Data System (ADS)

    Simón-Manso, Yamil; Neta, Pedatsur; Yang, Xiaoyu; Stein, Stephen E.

    2011-02-01

    While analyzing tandem mass spectra of tryptic tripeptides, intense unassigned peaks were observed, corresponding to neutral loss of 45 Da from a2 ions. This process was confirmed by MS3 experiments. Based on exact mass analysis, the loss was ascribed to (NH3 + CO) or formamide. The proposed mechanism involves a cyclic form of the a2 ions. The structure of the a2 - 45 ions was confirmed by their fragmentation in MS3 experiments. Loss of (NH3 + CO) from the a2 ions occurs in competition with other paths, such as the loss of H2O or the formation of immonium ions. However, if the a2 ion contains methionine, a neutral loss of 48 Da (ascribed to CH3SH) predominates, and is followed by the loss of (NH3 + CO). These processes were confirmed by MS3 experiments. The intensity of the a2 - 48 peak formed from XaaMet has a maximum value of 42% (of the total intensity of all ions) for Xaa=Gly, varies between 15% and 40% for most other Xaa residues, is lower for residues that can undergo loss of water or ammonia, and is very low for Lys or Arg. When the order of the residues is reversed to MetXaa, the loss of 48 Da is much smaller. This effect can be used to determine the sequence of b2 ions containing Met in proteomic studies. Considerable loss of CH3SH is observed from doubly protonated tryptic tripeptides with N-terminal Met, but the loss is much less when they are singly protonated or when Met is in the center position.

  1. (3H)domperidone binding to the kidney inner medullary collecting duct dopamine-2K (DA2K) receptor

    SciTech Connect

    Huo, T.; Healy, D.P. )

    1991-08-01

    Previous studies by the authors laboratory have indicated that inner medullary collecting ducts (IMCDs) express a novel dopamine (DA) receptor, designated DA2K, that is linked to stimulation of prostaglandin E2 production. This receptor has a distinct pharmacological profile and is similar in size, but not homologous to, the brain D2 receptor. Because the DA2-selective antagonist domperidone blocks DA-mediated stimulation of prostaglandin E2 production in IMCD cells, we utilized (3H)domperidone to study the binding characteristics of the DA2K receptor in IMCD cells. (3H)Domperidone binding was saturable and best fit to a single high density site (KD, 57.6 {plus minus} 10.5 nM; Bmax, 14.9 {plus minus} 2.7 pmol/mg protein). The specificity of (3H)domperidone binding in IMCD cells was verified by competition analysis with a variety of dopaminergic and nondopaminergic agents. Dopaminergic drugs were less potent competitors for (3H)domperidone binding in IMCD cells than previously reported for brain DA receptors, but the rank order was consistent with the labeling of a DA receptor (antagonists: domperidone greater than spiperone greater than haloperidol greater than Sch 23390 much greater than (-)-sulpiride; agonists: norapomorphine greater than fenoldopam much greater than dopamine = quinpirole), and was better correlated with the pharmacological profile for the brain D2 receptor than the brain D3 receptor. In addition, quinpirole, the most D3-selective ligand currently available, did not compete for (3H)domperidone binding in IMCD cells. These results add further support to the existence of a novel high density DA receptor, DA2K, expressed in IMCD cells.

  2. [The nuclear matrix proteins (mol. mass 38 and 50 kDa) are transported by chromosomes in mitosis].

    PubMed

    Murasheva, M I; Chentsov, Iu S

    2010-01-01

    It was shown by immunofluorescence method that serum M68 and serum K43 from patients with autoimmune disease stain interphase nuclei and periphery of mitotic chromosomes of pig kidney cells. Western blotting reveals the polypeptide with mol. mass of 50 kDa in serum M68, and the polypeptide with mol. mass of 38 kDa in serum K43. In the nuclear protein matrix, the antibodies to protein with mol. mass of 38 kDa stained only nucleolar periphery, while the antibodies to the protein with mol. mass of 50 kDa stained both the nucleolar periphery and all the interphase nucleus. It shows that among all components of nuclear protein matrix (lamina, internuclear network, residual nucleoli) only nucleolar periphery contains the 38 kDa protein, while the 50 kDa protein is a part of residual nucleolar periphery and takes part in nuclear protein network formation. In the interphase cells, both proteins were in situ localized in the nuclei, but one of them with mol. mass of 50 kDa was in the form of small clearly outlined granules, while the other (38 kDa) was in the form of small bright granules against the background of diffusely stained nuclei. Both proteins were also revealed as continuous ring around nucleolar periphery. During all mitotic stages, the 50 kDa protein was seen on the chromosomal periphery as a cover, and the 38 kDa protein formed separate fragments and granules around them. After nuclear and chromosome decondensation induced by hypotonic treatment, both antibodies stain interphase nuclei in diffuse manner, but in mitotic cells they stained the surface of the swollen chromosomes. The polypeptide with mol. mass of 50 kDa maintained strong connection with chromosome periphery both in norm and under condition of decondensation induced by hypotonic treatment and at subsequent recondensation in isotonic medium. In contrast, the protein with mol. mass of 38 kDa partially lost the contact with a chromosome during recondensation appearing also in the form of granules in

  3. Approaches to Sample Size Determination for Multivariate Data: Applications to PCA and PLS-DA of Omics Data.

    PubMed

    Saccenti, Edoardo; Timmerman, Marieke E

    2016-08-01

    Sample size determination is a fundamental step in the design of experiments. Methods for sample size determination are abundant for univariate analysis methods, but scarce in the multivariate case. Omics data are multivariate in nature and are commonly investigated using multivariate statistical methods, such as principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA). No simple approaches to sample size determination exist for PCA and PLS-DA. In this paper we will introduce important concepts and offer strategies for (minimally) required sample size estimation when planning experiments to be analyzed using PCA and/or PLS-DA. PMID:27322847

  4. Hunting for the Tristan plume - An upper mantle tomography around the volcanic island Tristan da Cunha

    NASA Astrophysics Data System (ADS)

    Schlömer, Antje; Geissler, Wolfram H.; Jokat, Wilfried; Jegen, Marion

    2016-04-01

    Tristan da Cunha is a volcanic island in the South Atlantic close to the Mid-Atlantic Ridge. It is part of an area consisting of widely scattered seamounts and small islands at the western and youngest end of the aseismic Walvis Ridge. Tristan da Cunha together with the Walvis Ridge represents the classical example of a mantle plume track, because of the connection to the Cretaceous Etendeka flood basalt province in NW Namibia. The genesis of the island has so far remained enigmatic. It is hotly debated, if Tristan da Cunha sits actually above a deep mantle plume or if it is only originated by upwelling material from weak (leaky) fracture zones. It also has to be clarified if there are any indications for a plume-ridge interaction. Geochemical investigations have shown complex compositions of magmatic samples from Tristan da Cunha, which could be interpreted as a mixing of plume-derived melts and depleted upper mantle sources. To improve our understanding about the origin of Tristan and to test the mantle plume hypothesis, we deployed 24 broadband ocean-bottom seismometers and 2 seismological land stations around and on the island during an expedition in January 2012 with the German research vessel Maria S. Merian. After acquiring continuous seismological data for almost one year, the seismometers were recovered in early January 2013. We cross-correlated the arrival times of teleseismic P and PKP phases to perform a finite-frequency tomography of the upper mantle beneath the study area. Here we show the 3D mantle structure in terms of velocity variations: We do not image a "classical" plume-like structure directly beneath Tristan da Cunha, but we observe regions of low velocities at the edges of our array that we relate to local mantle upwelling from potentially deeper sources. Additionally we discuss local seismicity within the Tristan da Cunha region, which show processes along the nearby mid-ocean ridge and transform faults. Furthermore, the local seismicity

  5. Estudo de não gaussianidade nas anisotropias da RCF medidas Wmap

    NASA Astrophysics Data System (ADS)

    Andrade, A. P. A.; Wuensche, C. A.; Ribeiro, A. L. B.

    2003-08-01

    A investigação do campo de flutuações da Radiação Cósmica de Fundo (RCF) pode oferecer um importante teste para os modelos cosmológicos que descrevem a origem e a evolução das flutuações primordiais. De um lado, apresenta-se o modelo inflacionário que prevê um espectro de flutuações adiabáticas distribuídas segundo uma gaussiana e, de outro, os modelos de defeitos topológicos (dentre outros) que descrevem um mecanismo para a geração de flutuações de isocurvatura que obedecem a uma distribuição não gaussiana. Este trabalho tem como objetivo caracterizar traços do modelo não gaussiano de campo misto (entre flutuações adiabáticas e de isocurvatura) nos mapas do Wilkinson Microwave Anisotropy Probe (WMAP). Simulações das anisotropias da RCF no contexto de mistura indicam traços marcantes na distribuição das flutuações de temperatura, mesmo quando consideradas pequenas contribuições do campo de isocurvatura (da ordem de 0.001). O efeito da mistura entre os campos resulta na transferência de potência de flutuações em escalas angulares intermediárias para flutuações em pequenas escalas angulares. Este efeito pode ser caracterizado pela relação entre as amplitudes dos primeiros picos acústicos no espectro de potência da RCF. Neste trabalho, investigamos a contribuição do campo de isocurvatura, no contexto de mistura, sobre as observações recentes da RCF realizadas pelo WMAP. As previsões do modelo de campo misto, uma vez confrontadas com as observações em pequenas escalas angulares, podem ajudar a revelar a natureza das flutuações primordiais.

  6. O Imperio como Argumento: Um Contraponto entre Joaquim Nabuco e o Bispo D. Jose Mauricio da Rocha (The Empire as Argument: A Counterpoint between Joaquim Nabuco and the Bishop Dom Jose Mauricio da Rocha).

    ERIC Educational Resources Information Center

    Marson, Izabel Andrade

    1998-01-01

    Dialogs with the previous article establishing a comparative analysis between the political acceptance present in the reactionary speech of Dom Jose Mauricio da Rocha and in the monarchist words of Joaquim Nabuco. (PA)

  7. Iron abundance in the hot DA white dwarfs Feige 24 and G191 B2B

    NASA Technical Reports Server (NTRS)

    Vennes, Stephane; Chayer, Pierre; Thorstensen, John R.; Bowyer, Stuart; Shipman, Harry L.

    1992-01-01

    Attention is given to model calculations of the far- and extreme-UV line spectra of highly ionized Fe species (Fe IV, Fe V, and Fe VI) for hot high-gravity H-rich stars. A spectral analysis of 31 hr of exposure of the DA white dwarf Feige 24 with IUE in the echelle mode reveals the presence of Fe with an abundance relative to H by number of (5-10) x 10 exp -6 with an uncertainty dominated by the determination of stellar parameters. An analysis of IUE data from the white dwarf G191 B2B results in a similar Fe abundance if this star shares similar atmospheric parameters (Teff, g) with Feige 24. Fe is thus the second most abundant photospheric element in hot DA white dwarfs.

  8. Crosslinking of hemin to a specific site on the 90-kDa ferritin repressor protein

    SciTech Connect

    Lin, Jihjing; Thach, R.E. ); Patino, M.M.; Gaffield, L.; Walden. W.E. ); Smith, A. )

    1991-07-15

    Incubation of a 90-kDa ferritin repressor protein (FRP) with small amounts of radiolabeled hemin resulted in the formation of a strong interaction between the two that was stable to SDS/PAGE. Of seven other proteins tested individually, only apohemopexin and bovine serum albumin showed similar crosslinking ability, albeit to a much lower extent. ({sup 14}C)Hemin specifically crosslinked to FRP in the presence of a 50-fold excess of total wheat germ proteins. Inclusion of catalase did not prevent the reaction of hemin with FRP, suggesting that H{sub 2}O{sub 2} is not involved. The subsequent addition of a stoichiometric amount of apohemopexin did not reverse the reaction. Exhaustive digestion of the complex with Staphylococcus aureus V8 protease produced a major labeled peptide of 17 kDa. These results show the existence of a highly specific, uniquely reactive hemin binding site on FRP.

  9. [Freud's identification with men who had 2 mothers: Oedipus, Leonardo da Vinci, Michealangelo and Moses].

    PubMed

    Harsch, H E

    1994-02-01

    In view of the fact that as a child Sigmund Freud was looked after by two mothers--his actual mother and a nursemaid--it is hardly surprising that traces of this pre-oedipal situation, fraught as it was with traumatisation and loss, should be discernible in the works of the creator of psychoanalysis. Freud's continued preoccupation with the Oedipus myth, his interest in "great men" like da Vinci and Michelangelo, and finally his identification with the figure of Moses are pointers not only to the paternal dimension (as long suggested by Freud's biographers) but also to the maternal dimension and its significance for Freud's life and work. The author demonstrates that those mythical and historical figures which Freud identified with--Oedipus, da Vinci, Michelangelo, Moses--themselves all had two mothers and sublimated this traumatic experience into outstanding achievements, the same being true of Freud himself "who solved the famous riddle and was a most powerful man". PMID:8153361

  10. Towards the Implementation of an Autonomous Camera Algorithm on the da Vinci Platform.

    PubMed

    Eslamian, Shahab; Reisner, Luke A; King, Brady W; Pandya, Abhilash K

    2016-01-01

    Camera positioning is critical for all telerobotic surgical systems. Inadequate visualization of the remote site can lead to serious errors that can jeopardize the patient. An autonomous camera algorithm has been developed on a medical robot (da Vinci) simulator. It is found to be robust in key scenarios of operation. This system behaves with predictable and expected actions for the camera arm with respect to the tool positions. The implementation of this system is described herein. The simulation closely models the methodology needed to implement autonomous camera control in a real hardware system. The camera control algorithm follows three rules: (1) keep the view centered on the tools, (2) keep the zoom level optimized such that the tools never leave the field of view, and (3) avoid unnecessary movement of the camera that may distract/disorient the surgeon. Our future work will apply this algorithm to the real da Vinci hardware. PMID:27046563

  11. PSI-O, a new 10-kDa subunit of eukaryotic photosystem I.

    PubMed

    Knoetzel, Jürgen; Mant, Alexandra; Haldrup, Anna; Jensen, Poul Erik; Scheller, Henrik Vibe

    2002-01-16

    A novel polypeptide with an apparent molecular mass of 9 kDa was detected after sodium dodecyl sulphate-polyacrylamide gel electrophoresis of Arabidopsis photosystem I (PSI) and was N-terminally sequenced. Corresponding cDNA clones encode a precursor protein of 140 amino acid residues which was imported into isolated intact chloroplasts and processed to the mature protein, designated PSI-O. The mature protein has two transmembrane helices and a calculated mass of 10104 Da. The PSI-O protein was also shown to be present in PSI isolated from barley and spinach, and was essentially absent in chloroplast grana. Expressed sequences encoding similar proteins are available from many species of plants and green algae. PMID:11801243

  12. A 32-kDa protein associated with phospholipase A2-inhibitory activity from human placenta.

    PubMed

    Hayashi, H; Owada, M K; Sonobe, S; Kakunaga, T; Kawakatsu, H; Yano, J

    1987-11-01

    Two monomeric 32-kDa proteins, termed 32K-I (pI 5.8) and 32K-II (pI 5.1), were isolated from human placenta, which was solubilized by a Ca2+-chelator. Only 32K-I was associated with PLA2-inhibitory activity. CNBr peptide mapping indicated that 32K-I was distinct from 32K-II and two 36-kDa proteins, called calpactin I and II or lipocortin II and I, which have been shown to possess PLA2-inhibitory activity. 32K-I bound to PS in a Ca2+-dependent manner. 32K-I was detected in many tissues except brain, cardiac and skeletal muscle. PMID:3666152

  13. Solid state of a new PDE-5 inhibitor DA-8159: characterization, dissolution, transformation.

    PubMed

    Ryu, Ji Yoon; Sohn, Young-Taek

    2012-05-01

    The polymorphic forms of a new PDE-5 inhibitor DA-8159 were prepared and characterized by differential scanning calorimetry (DSC), powder X-ray diffractometry (PXRD) and thermogravimetric analysis (TG). Two crystal forms and one amorphous form of DA-8159 have been isolated by recrystallization and characterized by DSC, TG and PXRD. From the TG data it was confirmed that two crystal forms are neither solvates nor hydrates. The PXRD patterns of the two crystal forms were different. In the dissolution studies in simulated intestinal fluid at 37 ± 0.5°C, the solubility decreased in the order of amorphous form > Form 1 > Form 2. After storage of 60 days, Form 1 was transformed to Form 2. Form 2 was not transformed. The amorphous form was transformed to Form 2 at 52% R.H. and 95% R.H., but it did not transform at 0% R.H. PMID:22644853

  14. Maximizing use of robot-arm no. 3 in daVinci-assisted thoracic surgery.

    PubMed

    Kajiwara, Naohiro; Maeda, Junichi; Yoshida, Koichi; Kato, Yasufumi; Hagiwara, Masaru; Kakihana, Masatoshi; Ohira, Tatsuo; Kawate, Norihiko; Ikeda, Norihiko

    2015-05-01

    We have previously reported on the importance of appropriate robot-arm settings and replacement of instrument ports in robot-assisted thoracic surgery, because the thoracic cavity requires a large space to access all lesions in various areas of the thoracic cavity from the apex to the diaphragm and mediastinum and the chest wall. (1 - 3) Moreover, it can be difficult to manipulate the da Vinci Surgical System using only arms No. 1 and No. 2 depending on the tumor location. However, arm No. 3 is usually positioned on the same side as arm No. 2, and sometimes it is only used as an assisting-arm to avoid conflict with other arms ( Fig. 1 ). In this report, we show how robot-arm No. 3 can be used with maximum effectiveness in da Vinci-assisted thoracic surgery. [Figure: see text]. PMID:26011219

  15. [Da Vinci robot-assisted thoracoscopy for primary hyperparathyroidism: a new application in endocrine surgery].

    PubMed

    Brunaud, L; Ayav, A; Bresler, L; Schjött, B

    2008-01-01

    Primary hyperparathyroidism is a relatively frequent disease whose incidence is often underestimated. It is caused by one or more hyperfunctioning parathyroid glands. Almost all pathologic glands (hyperplasia or adenoma) are located in the neck but 1-2% may be located in the mediastinum and may require a sternotomy or thoracotomy approach for resection. The thoracoscopic approach was proposed in 1994 and the DaVinci robotic system allows performance of this thoracoscopic procedure with the benefits of 3-dimensional vision, and improved surgical dexterity and ergonomics. We report a case of a patient with a parathyroid adenoma located in the aorto-pulmonary window which was resected using a left thoracoscopic approach aided by the Da Vinci robotic system. PMID:18645560

  16. Visual degradation in Leonardo da Vinci's iconic self-portrait: A nanoscale study

    NASA Astrophysics Data System (ADS)

    Conte, A. Mosca; Pulci, O.; Misiti, M. C.; Lojewska, J.; Teodonio, L.; Violante, C.; Missori, M.

    2014-06-01

    The discoloration of ancient paper, due to the development of oxidized groups acting as chromophores in its chief component, cellulose, is responsible for severe visual degradation in ancient artifacts. By adopting a non-destructive approach based on the combination of optical reflectance measurements and time-dependent density functional theory ab-initio calculations, we describe and quantify the chromophores affecting Leonardo da Vinci's iconic self-portrait. Their relative concentrations are very similar to those measured in modern and ancient samples aged in humid environments. This analysis quantifies the present level of optical degradation of the Leonardo da Vinci's self-portrait which, compared with future measurements, will assess its degradation rate. This is a fundamental information in order to plan appropriate conservation strategies.

  17. First experience DaTSCAN imaging using cadmium-zinc-telluride gamma camera SPECT.

    PubMed

    Farid, Karim; Queneau, Mathieu; Guernou, Mohamed; Lussato, David; Poullias, Xavier; Petras, Slavomir; Caillat-Vigneron, Nadine; Songy, Bernard

    2012-08-01

    We report our first experience of brain DaTSCAN SPECT imaging using cadmium-zinc-telluride gamma camera (CZT-GC) in 2 cases: a 64-year-old patient suffering from essential tremor and a 73-year-old patient presenting with atypical bilateral extrapyramidal syndrome. In both cases, 2 different acquisitions were performed and compared, using a double-head Anger-GC, followed immediately by a second acquisition on CZT-GC. There were no significant visual differences between images generated by different GC. Our first result suggests that DaTSCAN SPECT is feasible on CZT-GC, allowing both injected dose and acquisition time reductions without compromising image quality. This experience needs to be evaluated in larger series. PMID:22785531

  18. Development of noSQL data storage for the ATLAS PanDA Monitoring System

    NASA Astrophysics Data System (ADS)

    Potekhin, M.; ATLAS Collaboration

    2012-06-01

    For several years the PanDA Workload Management System has been the basis for distributed production and analysis for the ATLAS experiment at the LHC. Since the start of data taking PanDA usage has ramped up steadily, typically exceeding 500k completed jobs/day by June 2011. The associated monitoring data volume has been rising as well, to levels that present a new set of challenges in the areas of database scalability and monitoring system performance and efficiency. These challenges are being met with a R&D effort aimed at implementing a scalable and efficient monitoring data storage based on a noSQL solution (Cassandra). We present our motivations for using this technology, as well as data design and the techniques used for efficient indexing of the data. We also discuss the hardware requirements as they were determined by testing with actual data and realistic loads.

  19. VizieR Online Data Catalog: The Solar Twin Planet Search. IV. (dos Santos+, 2016)

    NASA Astrophysics Data System (ADS)

    Dos Santos, L. A.; Melendez, J.; Do Nascimento, J.-D. Jr; Bedell, M.; Ramirez, I.; Bean, J. L.; Asplund, M.; Spina, L.; Dreizler, S.; Alves-Brito, A.; Casagrande, L.

    2016-07-01

    Ages, the measured projected rotational velocities (vsini) and stellar parameters of the 81 solar twins and the Sun. The ages of all solar twins and stellar parameters for HIP68468 were obtained by Tucci Maia et al. (2016A&A...590A..32T). Stellar parameters for the other solar twins were obtained by Ramirez et al. (2014A&A...572A..48R). The vsini were measured by line profile fitting using red spectra from HARPS (R=115000). Macroturbulence velocities were inferred from the scaling Eq. 2 in our study. (1 data file).

  20. Source-rock evaluation of outcrop samples from Vanuatu (Malakula, Espiritu Santo, Maewo, and Pentecost)

    USGS Publications Warehouse

    Buchbinder, Binyamin; Halley, Robert B.

    1988-01-01

    The samples collected for the present study represent only a portion of the sedimentary column in the various sedimentary basins of Vanuatu.  The characterize only the outer margins of the sedimentary basins and do not necessarily reflect the source-rock potential of the deeper (offshore) parts of the basins.

  1. Groundwater Resources of Ribeira Paul Basin, Island of Santo Antao, Cape Verde, West Africa

    USGS Publications Warehouse

    Heilweil, Victor M.; Gingerich, Stephen B.; Verstraeten, Ingrid M.

    2010-01-01

    Groundwater resources in Cape Verde provide water for agriculture, industry, and human consumption. These resources are limited and susceptible to contamination. Additional groundwater resources are needed for continued agricultural development, particularly during times of drought, but increased use and (or) climatic change may have adverse effects on the quantity and quality of freshwater available. In volcanic island aquifers such as those of Cape Verde, a lens of fresh groundwater typically ?floats? upon a layer of brackish water at the freshwater/saltwater boundary, and increased pumping may cause salt water intrusion or other contamination. A recent U.S. Geological Survey study assessed baseline groundwater conditions in watersheds on three islands of Cape Verde to provide the scientific basis for sustainably developing water resources and minimizing future groundwater depletion and contamination.

  2. VizieR Online Data Catalog: SWEETCat I. Stellar parameters for host stars (Santos+, 2013)

    NASA Astrophysics Data System (ADS)

    Santos, N. C.; Sousa, S. G.; Mortier, A.; Neves, V.; Adibekyan, V.; Tsantaki, M.; Delgado Mena, E.; Bonfils, X.; Israelian, G.; Mayor, M.; Udry; S.

    2013-07-01

    The file sweetcat.dat contains the spectroscopic parameters for all the planet hosts compiled for this work (data as of July 2013). Up to date tables can be found at http://www.astro.up.pt/resources/sweet-cat . The spectra were gathered through observations, made by our team, and by the use of the ESO archive. In total, six different spectrographs were used: FEROS (2.2m ESO/MPI telescope, La Silla, Chile), FIES (Nordic Optical Telescope, La Palma, Spain), HARPS (3.6m ESO telescope, La Silla, Chile), SARG (TNG Telescope, La Palma, Spain), SOPHIE (1.93m telescope, OHP, France), and UVES (VLT Kueyen telescope, Paranal, Chile). (2 data files).

  3. "Being Present in the World": A Conversation with Professor Jose Marques dos Santos

    ERIC Educational Resources Information Center

    Katz, Richard N.; Dodds, Ted

    2008-01-01

    The University of Porto was awarded the European University Information Systems (EUNIS) Elite Award for its innovative application of information technology to a European university. This university--nearly one hundred years old--is pressing hard, on a number of fronts, to place itself in the top rank of European universities. The Rector of the…

  4. On the interpretation of the Exosat photometry of two hot DA white dwarfs

    NASA Technical Reports Server (NTRS)

    Vennes, Stephane; Shipman, Harry L.; Petre, Robert

    1990-01-01

    Exosat photometric observations of the hot DA white dwarfs GD 2 and EG 70 are presented and analyzed using model atmosphere and synthetic spectrum calculations. The numerical accuracy of the calculations and their impact on the reliability of the results are addressed. The optimum atmospheric parameters that explain the complete spectral coverage are derived. The consequences of these conjectures for the spectral evolution theory of white dwarf stars are considered.

  5. 76 FR 19903 - Special Conditions: Diamond Aircraft Industry Model DA-40NG; Diesel Cycle Engine

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ...These special conditions are issued for the Diamond Aircraft Industry (DAI) GmbH model DA-40NG the Austro Engine GmbH model E4 aircraft diesel engine (ADE) using turbine (jet) fuel. This airplane will have a novel or unusual design feature(s) associated with the installation of a diesel cycle engine utilizing turbine (jet) fuel. The applicable airworthiness regulations do not contain adequate......

  6. STRESS AND FAILURE ANALYSIS OF RAPIDLY ROTATING ASTEROID (29075) 1950 DA

    SciTech Connect

    Hirabayashi, Masatoshi; Scheeres, Daniel J.

    2015-01-01

    Rozitis et al. recently reported that near-Earth asteroid (29075) 1950 DA, whose bulk density ranges from 1.0 g cm{sup –3} to 2.4 g cm{sup –3}, is a rubble pile and requires a cohesive strength of at least 44-76 Pa to keep from failing due to its fast spin period. Since their technique for giving failure conditions required the averaged stress over the whole volume, it discarded information about the asteroid's failure mode and internal stress condition. This paper develops a finite element model and revisits the stress and failure analysis of 1950 DA. For the modeling, we do not consider material hardening and softening. Under the assumption of an associated flow rule and uniform material distribution, we identify the deformation process of 1950 DA when its constant cohesion reaches the lowest value that keeps its current shape. The results show that to avoid structural failure the internal core requires a cohesive strength of at least 75-85 Pa. It suggests that for the failure mode of this body, the internal core first fails structurally, followed by the surface region. This implies that if cohesion is constant over the whole volume, the equatorial ridge of 1950 DA results from a material flow going outward along the equatorial plane in the internal core, but not from a landslide as has been hypothesized. This has additional implications for the likely density of the interior of the body.

  7. Purification and properties of an oestrogen-stimulated mouse uterine glycoprotein (approx. 70 kDa).

    PubMed Central

    Teng, C T; Walker, M P; Bhattacharyya, S N; Klapper, D G; DiAugustine, R P; McLachlan, J A

    1986-01-01

    An oestrogen-induced secretory protein from mouse uterine luminal fluid was purified by CM-Affi-Gel Blue chromatography and reverse-phase h.p.l.c. This protein has an apparent molecular mass of approx. 70 kDa both by SDS/polyacrylamide-gel electrophoresis (with or without 2-mercaptoethanol) and by gel-filtration column chromatography, indicating that it exists as a single-chain polypeptide. Further analysis of the protein revealed that it is highly basic (pI greater than or equal to 10) and is a glycoprotein. The N-terminus appears to be blocked to Edman degradation. The partial amino acid sequence of a fragment was obtained by cleavage with CNBr; no sequence homology was apparent between the analysed fragment and other known sequences. The incorporation of [35S]methionine into uterine proteins in vitro revealed that oestrogen treatment of immature mice stimulates both synthesis and secretion of the 70 kDa protein. An enzyme-linked immunosorbent assay with polyclonal antibody was used to determine the tissue distribution of the protein. Tissues such as lung, brain, spleen, muscle, intestine, liver, kidney and ovary of oestrogen-treated mice did not have detectable amounts of the 70 kDa protein. Immunoreactivity was present in uterine and vaginal tissues from oestrogen-treated animals. The 70 kDa protein was not induced by testosterone or progesterone. Although the function of this protein is unknown, it is useful as a marker for the study of oestrogen action in the mammalian uterus as well as regulation of gene expression at the molecular level. Images Fig. 1. Fig. 2. Fig. 3. Fig. 4. Fig. 6. PMID:3814091

  8. The Handedness of Leonardo da Vinci: A Tale of the Complexities of Lateralisation

    ERIC Educational Resources Information Center

    McManus, I. C.; Drury, Helena

    2004-01-01

    The handedness of Leonardo da Vinci is controversial. Although there is little doubt that many of his well-attributed drawings were drawn with the left hand, the hatch marks of the shading going downwards from left to right, it is not clear that he was a natural left-hander, there being some suggestion that he may have become left-handed as the…

  9. Diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT: a clinical follow up study

    PubMed Central

    Menéndez-González, Manuel; Tavares, Francisco; Zeidan, Nahla; Salas-Pacheco, José M.; Arias-Carrión, Oscar

    2014-01-01

    The [123I]ioflupane—a dopamine transporter radioligand—SPECT (DaT-SPECT) has proven to be useful in the differential diagnosis of tremor. Here, we investigate the diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT. Therefore, 30 patients with tremor and normal DaT-SPECT were followed up for 2 years. In 18 cases we were able to make a diagnosis. The residual 12 patients underwent a second DaT-SPECT, were then followed for additional 12 months and thereafter the diagnosis was reconsidered again. The final diagnoses included cases of essential tremor, dystonic tremor, multisystem atrophy, vascular parkinsonism, progressive supranuclear palsy, corticobasal degeneration, fragile X–associated tremor ataxia syndrome, psychogenic parkinsonism, iatrogenic parkinsonism and Parkinson's disease. However, for 6 patients the diagnosis remained uncertain. Larger series are needed to better establish the relative frequency of the different conditions behind these cases. PMID:24744729

  10. Laparoscopically assisted total daVinci aorto bifemoral graft bypass with a unique system of graft delivery.

    PubMed

    Martinez, B D; George, P M; Ameer, A; Gerhardinger, A M; Moses, J T; Hansen, J M

    2009-03-01

    We report the first known example of a fully robotic aortobifemoral graft and aortic endarterectomy using the daVinci Surgical System and a unique method for graft delivery using the Endo-Vein Harvester. PMID:18657387

  11. Diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT: a clinical follow up study.

    PubMed

    Menéndez-González, Manuel; Tavares, Francisco; Zeidan, Nahla; Salas-Pacheco, José M; Arias-Carrión, Oscar

    2014-01-01

    The [(123)I]ioflupane-a dopamine transporter radioligand-SPECT (DaT-SPECT) has proven to be useful in the differential diagnosis of tremor. Here, we investigate the diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT. Therefore, 30 patients with tremor and normal DaT-SPECT were followed up for 2 years. In 18 cases we were able to make a diagnosis. The residual 12 patients underwent a second DaT-SPECT, were then followed for additional 12 months and thereafter the diagnosis was reconsidered again. The final diagnoses included cases of essential tremor, dystonic tremor, multisystem atrophy, vascular parkinsonism, progressive supranuclear palsy, corticobasal degeneration, fragile X-associated tremor ataxia syndrome, psychogenic parkinsonism, iatrogenic parkinsonism and Parkinson's disease. However, for 6 patients the diagnosis remained uncertain. Larger series are needed to better establish the relative frequency of the different conditions behind these cases. PMID:24744729

  12. Fully digital controlled A.C. servo engraving machine based on DEC4DA

    NASA Astrophysics Data System (ADS)

    Shu, Zhibing; Chen, Xianfeng; Zhang, Hairong; Huang, Yiqun; Yan, Caizhong

    2005-12-01

    A novel engraving machine (NUT-1A) is presented, in which fully digital controlled AC system based on DEC4DA was used to improve the machining precision and sensitivity. This engraving machine was constructed around AC servo motor with encoder, controlled by a servo motor control card - DEC4DA. As the upper unit of AC servo motor, DEC4DA was a numerical control generator, which received pulses form CPU by ISA bus, and these pulses were amplified and converted to drive AC servo actuator. This novel engraving machine can achieve a higher positioning accuracy of +/-0.01mm and positioning repetition of +/-0.005mm, and its resolution is 0.001mm/0.0001mm. Moreover, because of multi-closed loops were used in the system, the steady and transient performances are more excellent. This system ensures a much quicker current regulation in closed-loop operation, of acceleration and braking in both directions, as well as stable speed characteristics. Amplifier boards are protected against excessive current, excessive temperature and short circuiting of the motor supply cables.

  13. A 115 kDa calmodulin-binding protein is located in rat liver endosome fractions.

    PubMed Central

    Enrich, C; Bachs, O; Evans, W H

    1988-01-01

    The distribution of calmodulin-binding polypeptides in various rat liver subcellular fractions was investigated. Plasma-membrane, endosome, Golgi and lysosome fractions were prepared by established procedures. The calmodulin-binding polypeptides present in the subcellular fractions were identified by using an overlay technique after transfer from gels to nitrocellulose sheets. Distinctive populations of calmodulin-binding polypeptides were present in all the fractions examined except lysosomes. A major 115 kDa calmodulin-binding polypeptide of pI 4.3 was located to the endosome subfractions, and it emerges as a candidate endosome-specific protein. Partitioning of endosome fractions between aqueous and Triton X-114 phases indicated that the calmodulin-binding polypeptide was hydrophobic. Major calmodulin-binding polypeptides of 140 and 240 kDa and minor polypeptides of 40-60 kDa were present in plasma membranes. The distribution of calmodulin in the various endosome and plasma-membrane fractions was also analysed, and the results indicated that the amounts were high compared with those in the cytosol. Images Fig. 1. Fig. 2. Fig. 3. Fig. 4. Fig. 5. PMID:3214436

  14. The validity of the autism spectrum disorders-diagnosis for intellectually disabled adults (ASD-DA).

    PubMed

    Matson, Johnny L; Wilkins, Jonathan; Boisjoli, Jessica A; Smith, Kimberly R

    2008-01-01

    Psychologists interviewed direct-care staff using a battery of assessment measures including the autism spectrum disorders-diagnosis for intellectually disabled adults (ASD-DA), the Diagnostic Assessment for the Severely Handicapped-II (DASH-II), the Matson Evaluation of Social Skills for Individuals with Severe Retardation (MESSIER), the Socialization domain of the Vineland Adaptive Behavior Scales (VABS), and a checklist containing criteria for autism and PDD-NOS from the DSM-IV-TR and ICD-10. Three hundred and seven intellectually disabled (ID) adolescents and adults ranging in age from 16 to 88 were assessed. Participants were diagnosed with either ID and ASD (autism or PDD-NOS; n=156) or ID and no Axis I diagnosis (n=151). A modification of the multitrait-multimethod approach was used to establish the convergent and discriminant validity of the ASD-DA. The scale proved to have robust convergent validity when correlated with the DSM-IV-TR/ICD-10 checklist, MESSIER, and Socialization domain of the VABS. Additionally, discriminant validity was demonstrated by comparing the ASD-DA to items from the DASH-II (measure of general psychopathology). The implications of these data are discussed. PMID:17981435

  15. Petrology of potassic alkaline ultramafic and carbonatitic rocks from Planalto da Serra (Mato Grosso State), Brazil

    NASA Astrophysics Data System (ADS)

    Comin-Chiaramonti, Piero; Gomes, Celso B.; De Min, Angelo; Ruberti, Excelso; Girardi, Vicente A. V.; Slejko, Francesca; Neder, Renato D.; Pinho, Francisco E. C.

    2014-12-01

    The Planalto da Serra igneous rocks form plugs, necks and dykes of carbonate-rich ultramafic lamprophyres (aillikites and glimmerites with kamafugitic affinity) and carbonatites (alvikites and beforsites). Phlogopite and/or tetraphlogopite, diopside and melanitic garnet are restricted to aillikitic rock-types, whereas pyroclore occurs only in carbonatites. Aillikites and carbonatites are altered to hydrotermalites, having chlorite and serpentine as dominant minerals. Planalto da Serra igneous rock association has kamafugitic affinity (i.e. effusive, ultrapotassic. High LREE/HREE fractionation, incompatible elements data and Sr-Nd isotopes, suggest that the K-ultramafic alkaline and carbonatite rocks originated from a variably metasomatized mantle source enriched in radiogenic Sr. Crustal contamination is negligible or absent. Age values of 600 Ma rule out the geochronological relationship between the investigated intrusions and the Mesozoic alkaline bodies from the Azimuth 125° lineament. The TDM model ages allow to conclude that Planalto da Serra magma is derived from the partial melting of a mantle source metasomatised by K-rich carbonatated melt during the Early to Late Neoproterozoic. On the basis of alkaline magmatism repetitions at 600 Ma and 90-80 Ma we question the subsistence of a stationary mantle plume for so long time.

  16. da Vinci robot-assisted keyhole neurosurgery: a cadaver study on feasibility and safety.

    PubMed

    Marcus, Hani J; Hughes-Hallett, Archie; Cundy, Thomas P; Yang, Guang-Zhong; Darzi, Ara; Nandi, Dipankar

    2015-04-01

    The goal of this cadaver study was to evaluate the feasibility and safety of da Vinci robot-assisted keyhole neurosurgery. Several keyhole craniotomies were fashioned including supraorbital subfrontal, retrosigmoid and supracerebellar infratentorial. In each case, a simple durotomy was performed, and the flap was retracted. The da Vinci surgical system was then used to perform arachnoid dissection towards the deep-seated intracranial cisterns. It was not possible to simultaneously pass the 12-mm endoscope and instruments through the keyhole craniotomy in any of the approaches performed, limiting visualization. The articulated instruments provided greater dexterity than existing tools, but the instrument arms could not be placed in parallel through the keyhole craniotomy and, therefore, could not be advanced to the deep cisterns without significant clashing. The da Vinci console offered considerable ergonomic advantages over the existing operating room arrangement, allowing the operating surgeon to remain non-sterile and seated comfortably throughout the procedure. However, the lack of haptic feedback was a notable limitation. In conclusion, while robotic platforms have the potential to greatly enhance the performance of transcranial approaches, there is strong justification for research into next-generation robots, better suited to keyhole neurosurgery. PMID:25516094

  17. New Insights into the Problem of the Surface Gravity Distribution of Cool DA White Dwarfs

    NASA Astrophysics Data System (ADS)

    Tremblay, P.-E.; Bergeron, P.; Kalirai, J. S.; Gianninas, A.

    2010-04-01

    We review at length the longstanding problem in the spectroscopic analysis of cool hydrogen-line (DA) white dwarfs (T eff< 13,000 K) where gravities are significantly higher than those found in hotter DA stars. The first solution that has been proposed for this problem is a mild and systematic helium contamination from convective mixing that would mimic the high gravities. We constrain this scenario by determining the helium abundances in six cool DA white dwarfs using high-resolution spectra from the Keck I 10 m telescope. We obtain no detections, with upper limits as low as He/H = 0.04 in some cases. This allows us to put this scenario to rest for good. We also extend our model grid to lower temperatures using improved Stark profiles with non-ideal gas effects from Tremblay & Bergeron and find that the gravity distribution of cool objects remains suspiciously high. Finally, we find that photometric masses are, on average, in agreement with expected values, and that the high-log g problem is so far unique to the spectroscopic approach.

  18. NEW INSIGHTS INTO THE PROBLEM OF THE SURFACE GRAVITY DISTRIBUTION OF COOL DA WHITE DWARFS

    SciTech Connect

    Tremblay, P.-E.; Bergeron, P.; Gianninas, A.; Kalirai, J. S. E-mail: bergeron@astro.umontreal.c E-mail: gianninas@astro.umontreal.c

    2010-04-01

    We review at length the longstanding problem in the spectroscopic analysis of cool hydrogen-line (DA) white dwarfs (T{sub eff}< 13,000 K) where gravities are significantly higher than those found in hotter DA stars. The first solution that has been proposed for this problem is a mild and systematic helium contamination from convective mixing that would mimic the high gravities. We constrain this scenario by determining the helium abundances in six cool DA white dwarfs using high-resolution spectra from the Keck I 10 m telescope. We obtain no detections, with upper limits as low as He/H = 0.04 in some cases. This allows us to put this scenario to rest for good. We also extend our model grid to lower temperatures using improved Stark profiles with non-ideal gas effects from Tremblay and Bergeron and find that the gravity distribution of cool objects remains suspiciously high. Finally, we find that photometric masses are, on average, in agreement with expected values, and that the high-log g problem is so far unique to the spectroscopic approach.

  19. Shark cartilage 14 kDa protein as a dendritic cells activator.

    PubMed

    Safari, Elahe; Hassan, Zuhair M; Moazzeni, Seyed Mohammad

    2015-04-01

    Low molecular weight components of shark cartilage are reported to have anti-tumor as well as immuno-stimulating effects. Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that have a key role in establishment of anti-cancer immune response. In this study, the effect of 14 kDa protein from shark cartilage was investigated on stimulation and maturation of dendritic cells. The isolated 14 kDa protein from shark cartilage extract was added to DCs medium during overnight culture and their maturation and T cells stimulation potential was investigated. The majority of shark-cartilage-treated DCs expressed higher levels of maturation markers and were more effective in stimulation of allogenic T cells compared with non-treated DCs (p < 0.05). Our results showed that shark cartilage 14 kDa protein can potentially be used in DC-mediated T-cells stimulation and induction of desirable immune responses in clinical trials such as cancer immunotherapy. However, further studies are required to examine this proposal. PMID:25669314

  20. OnDA: online data analysis and feedback for serial X-ray imaging1

    PubMed Central

    Mariani, Valerio; Morgan, Andrew; Yoon, Chun Hong; Lane, Thomas J.; White, Thomas A.; O’Grady, Christopher; Kuhn, Manuela; Aplin, Steve; Koglin, Jason; Barty, Anton; Chapman, Henry N.

    2016-01-01

    This article describes a free and open-source data analysis utility designed for fast online feedback during serial X-ray diffraction and scattering experiments: OnDA (online data analysis). Three complete real-time monitors for common types of serial X-ray imaging experiments are presented. These monitors are capable of providing the essential information required for quick decision making in the face of extreme rates of data collection. In addition, a set of modules, functions and algorithms that allow developers to modify the provided monitors or develop new ones are provided. The emphasis here is on simple, modular and scalable code that is based on open-source libraries and protocols. OnDA monitors have already proven to be invaluable tools in several experiments, especially for scoring and monitoring of diffraction data during serial crystallography experiments at both free-electron laser and synchrotron facilities. It is felt that in the future the kind of fast feedback that OnDA monitors provide will help researchers to deal with the expected very high throughput data flow at next-generation facilities such as the European X-ray free-electron laser. PMID:27275150

  1. Albumin interacts specifically with a 60-kDa microvascular endothelial glycoprotein.

    PubMed Central

    Schnitzer, J E; Carley, W W; Palade, G E

    1988-01-01

    Confluent monolayers of microvascular endothelial cells, derived from the rat epididymal fat pad and grown in culture, were radioiodinated by using the lactoper-oxidase method. Their radioiodinated surface polypeptides were detected by NaDodSO4/PAGE (followed by autoradiography) and were characterized by both lectin affinity chromatography and protease digestion to identify the proteins involved in albumin binding. All detected polypeptides were sensitive to Pronase digestion, whereas several polypeptides were resistant to trypsin. Pronase treatment of the cell monolayer significantly reduced the specific binding of radioiodinated rat serum albumin, but trypsin digestion did not. Limax flavus, Ricinus communis, and Triticum vulgaris agglutinins competed significantly with radioiodinated rat serum albumin binding, whereas other lectins did not. A single 60-kDa glyco-protein was precipitated in common by these three lectins and was trypsin-resistant and Pronase-sensitive. Rat serum albumin affinity chromatography columns weakly but specifically bound a 60-kDa polypeptide from cell lysates derived from radioiodinated cell monolayers. These findings indicate that the 60-kDa glycoprotein is directly involved in a specific interaction of albumin with the cultured microvascular endothelial cells used in these experiments. Images PMID:3413125

  2. 32 CFR Appendix C to Part 623 - Agreement for Loan of US Army Materiel (DA Form 4881-R)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Agreement for Loan of US Army Materiel (DA Form... ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. C Appendix C to Part 623—Agreement for Loan of US Army Materiel (DA Form 4881-R) EC24OC91.013 EC24OC91.014 EC24OC91.015 EC24OC91.016...

  3. 32 CFR Appendix C to Part 623 - Agreement for Loan of US Army Materiel (DA Form 4881-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Agreement for Loan of US Army Materiel (DA Form... ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. C Appendix C to Part 623—Agreement for Loan of US Army Materiel (DA Form 4881-R) EC24OC91.013 EC24OC91.014 EC24OC91.015 EC24OC91.016...

  4. Evaluation of the immunomodulatory effect of the 14 kDa protein isolated from aged garlic extract on dendritic cells.

    PubMed

    Ahmadabad, Hasan Namdar; Hassan, Zuhair Mohammad; Safari, Elahe; Bozorgmehr, Mahmood; Ghazanfari, Tooba; Moazzeni, Seyed Mohammad

    2011-01-01

    Garlic is used all over the world for treatment of different diseases. A wide range of biological activities of garlic has been verified in vitro and in vivo. One of major proteins of garlic which has been isolated and purified is the 14 kDa protein. This protein has been shown to have immunomodulatory effects. In this study, the effect of the 14 kDa protein isolated from aged garlic extract (AGE) was investigated on maturation and immunomodulatory activity of dendritic cells (DC). Proteins were purified from AGE by biochemical method; the semi-purified 14 kDa protein was run on gel filtration Sephadex G50 and its purity was checked by SDS-PAGE. DC were isolated from spleen of BALB/c mice by Nycodenz centrifugation and their adhesiveness to plastic dish. 14 kDa protein from AGE was added to overnight culture of DC medium and the expression percentage of CD40, CD86, and MHC-II was evaluated by flowcytometric analysis. Also, proliferation of T-cells was measured by allogenic mixed lymphocyte reaction (MLR) test. The purified 14 kDa protein isolated from AGE increased the expression of CD40 molecule on DC, but it did not influence CD86 and MHCII molecules. Furthermore, no significant differences were noticed in the pulsed-DC with 14 kDa protein and non-pulsed DC on the MLR. PMID:21570063

  5. Histopathological effects and determination of the putative receptor of Bacillus thuringiensis Cry1Da toxin in Spodoptera littoralis midgut.

    PubMed

    BenFarhat-Touzri, Dalel; Saadaoui, Marwa; Abdelkefi-Mesrati, Lobna; Saadaoui, Imen; Azzouz, Hichem; Tounsi, Slim

    2013-02-01

    Bacillus thuringiensis subsp. aizawai strain HD133, known by its effectiveness against Spodoptera species, produces many insecticidal proteins including Cry1Ab, Cry1Ca and Cry1Da. In the present study, the insecticidal activity of Cry1Da against Spodoptera littoralis was investigated. It showed toxicity with an LC(50) of 224.4 ng/cm(2) with 95% confidence limits of (178.61-270.19) and an LC(90) of 467.77 ng/cm(2) with 95% confidence limits of (392.89-542.65). The midgut histopathology of Cry1Da fed larvae showed vesicle formation in the apical region, vacuolization and destruction of epithelial cells. Biotinylated-activated Cry1Da toxin bound protein of about 65 kDa on blots of S. littoralis brush border membrane preparations. This putative receptor differs in molecular size from those recognized by Cry1C and Vip3A which are active against this polyphagous insect. This difference in midgut receptors strongly supports the use of Cry1Da as insecticidal agent, particularly in case of Cry and/or Vip-resistance management. PMID:23220238

  6. AN IMPROVED SPECTROSCOPIC ANALYSIS OF DA WHITE DWARFS FROM THE SLOAN DIGITAL SKY SURVEY DATA RELEASE 4

    SciTech Connect

    Tremblay, P.-E.; Bergeron, P.; Gianninas, A. E-mail: bergeron@astro.umontreal.ca

    2011-04-01

    We present an improved spectroscopic and photometric analysis of hydrogen-line DA white dwarfs from the Sloan Digital Sky Survey Data Release 4 (SDSS DR4) based on model atmospheres that include improved Stark broadening profiles with non-ideal gas effects. We also perform a careful visual inspection of all spectroscopic fits with high signal-to-noise ratios (S/Ns > 12) and present improved atmospheric parameters (T{sub eff} and log g) for each white dwarf. Through a comparison of spectroscopic and photometric temperatures, we report the discovery of 35 DA+DB/DC double degenerate candidates and two helium-rich DA stars. We also determine that a cutoff at S/N = 15 optimizes the size and quality of the sample for computing the mean mass of DA white dwarfs, for which we report a value of 0.613 M{sub sun}. We compare our results to previous analyses of the SDSS DR4 and find a good agreement if we account for the shift produced by the improved Stark profiles. Finally, the properties of DA white dwarfs in the SDSS are weighed against those of the Villanova White Dwarf Catalog sample of Gianninas et al. We find systematically lower masses (by about 3% on average), a difference that we trace back to the data reduction procedure of the SDSS. We conclude that a better understanding of these differences will be important to determine the absolute temperature scale and mean mass of DA white dwarfs.

  7. Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells.

    PubMed Central

    Sirito, M; Lin, Q; Maity, T; Sawadogo, M

    1994-01-01

    USF is a helix-loop-helix transcription factor that, like Myc, recognizes the DNA binding motif CACGTG. Two different forms of USF, characterized by apparent molecular weights of 43,000 and 44,000, were originally identified in HeLa cells by biochemical analysis. Clones for the 43-kDa USF were first characterized, but only partial clones for the human 44-kDa USF (USF2, or FIP) have been reported. Here we describe a complete cDNA for the 44-kDa USF from murine cells. Analysis of this clone has revealed that the various USF family members are quite divergent in their N-terminal amino acid sequences, while a high degree of conservation characterizes their dimerization and DNA-binding domains. Interestingly, the 3' noncoding region of the 44-kDa USF cDNAs displayed an unusual degree of conservation between human and mouse. In vitro transcription/translation experiments indicated a possible role for this region in translation regulation. Alternative splicing forms of the 44-kDa USF messages exist in both mouse and human. Examination of the tissue and cell-type distribution of USF by Northern blot and gel retardation assays revealed that while expression of both the 43- and 44-kDa USF species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cells. Images PMID:8127680

  8. The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs.

    PubMed Central

    Takagaki, Y; MacDonald, C C; Shenk, T; Manley, J L

    1992-01-01

    Cleavage stimulation factor is one of the multiple factors required for 3'-end cleavage of mammalian pre-mRNAs. We have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that the 64-kDa subunit can be UV-crosslinked to RNA in a polyadenylylation signal (AAUAAA)-dependent manner. We have now isolated cDNAs encoding the 64-kDa subunit of human cleavage stimulation factor. The 64-kDa subunit contains a ribonucleoprotein-type RNA binding domain in the N-terminal region and a repeat structure in the C-terminal region in which a pentapeptide sequence (consensus MEARA/G) is repeated 12 times and the formation of a long alpha-helix stabilized by salt bridges is predicted. An approximately 270-amino acid segment surrounding this repeat structure is highly enriched in proline and glycine residues (approximately 20% for each). When cloned 64-kDa subunit was expressed in Escherichia coli, an N-terminal fragment containing the RNA binding domain bound to RNAs in a polyadenylylation-signal-independent manner, suggesting that the RNA binding domain is directly involved in the binding of the 64-kDa subunit to pre-mRNAs. Images PMID:1741396

  9. Identification and purification of a novel 120-kDa protein that recognizes the cAMP-responsive element

    SciTech Connect

    Andrisani, O.; Dixon, J.E. )

    1990-02-25

    The TGACGTCA (CRE) motif required for function by a number of cellular (somatostatin, enkephalin, alpha-human chorionic gonadotropin) and viral (Ad5 E1A-inducible, HTLV-1 TAX-inducible) genes is the site of interaction of multiple sequence-specific complexes. A protocol has been developed for the fractionation and purification of these activities. We report here the purification from HeLa nuclear extracts of a novel 120-kDa polypeptide which by Southwestern blots, gel retardation, and UV cross-linking assays displays CRE-specific binding. The CRE-affinity purified 120-kDa protein displays properties distinct from those of the 43-kDa CREB/ATF polypeptide. The 120-kDa protein is readily phosphorylated in vitro by protein kinase C but not by protein kinase A, suggesting that this molecule may mediate cellular signals distinct from the cAMP-responsive pathway. In vitro transcription-complementation assays utilizing the purified 120-kDa protein failed to transactivate the cAMP-responsive somatostatin promoter suggesting that the mode of action of this 120-kDa polypeptide may require an activation step distinct from the cAMP-signaling pathway.

  10. Atmospheric parameters of a soft X ray selected set of hot DA white dwarfs

    NASA Astrophysics Data System (ADS)

    Kidder, Kenneth Mark

    Soft x-ray/EUV observations provide a sensitive means of probing the structure and composition of the atmospheres of hot hydrogen-rich (DA) white dwarf stars. This is due to the fact that soft x-rays originate from hotter, deeper layers of the photosphere than do longer wavelengths. A primary aim of this research is to expand the sample of DA's observed with soft x-ray instrumentation through a search for serendipitous observations of catalogued hot DA white dwarfs in existing soft x-ray databases. The positional coincidences of 31 catalogued DA's (McCook and Sion 1987) were examined in Einstein IPC and EXOSAT LE fields. As a result, three soft x-ray sources have been identified corresponding to the white dwarfs (WD0631 + 107, WD1113 + 413 and WD0425 + 168) and a fourth source which is probably not due to photospheric thermal emission from the coincident white dwarf. The three detected DA's have relatively low effective temperatures (27,200 +/- 400 K, 26,200 +/- 1100 K and 24,000 +/- 500 K, respectively), as determined independently using complementary optical and UV spectroscopy. Applying these temperature constraints to the soft x-ray photometric data, the photospheres of these stars must be composed of effectively pure hydrogen (n(He)/n(H) less than 10 exp -5), although the hottest star, WD0631 + 107, can have trace levels of homogeneously mixed helium less than 10 exp -4.2. The analysis of the soft x-ray observations are supplemented with independent determinations of temperature, gravity, and V magnitude. The method of determination employed is to fit the well-sampled wings of the broad hydrogen absorption profiles with self-consistent model atmosphere predictions. Precise temperatures and gravities are obtained by fitting the observed profiles independently, then jointly determining a consistent solution for each object. In this effort, a ground-based observation program was established to obtain high quality spectra of the Balmer delta and gamma lines and in

  11. OpenDA Open Source Generic Data Assimilation Environment and its Application in Process Models

    NASA Astrophysics Data System (ADS)

    El Serafy, Ghada; Verlaan, Martin; Hummel, Stef; Weerts, Albrecht; Dhondia, Juzer

    2010-05-01

    Data Assimilation techniques are essential elements in state-of-the-art development of models and their optimization with data in the field of groundwater, surface water and soil systems. They are essential tools in calibration of complex modelling systems and improvement of model forecasts. The OpenDA is a new and generic open source data assimilation environment for application to a choice of physical process models, applied to case dependent domains. OpenDA was introduced recently when the developers of Costa, an open-source TU Delft project [http://www.costapse.org; Van Velzen and Verlaan; 2007] and those of the DATools from the former WL|Delft Hydraulics [El Serafy et al 2007; Weerts et al. 2009] decided to join forces. OpenDA makes use of a set of interfaces that describe the interaction between models, observations and data assimilation algorithms. It focuses on flexible applications in portable systems for modelling geophysical processes. It provides a generic interfacing protocol that allows combination of the implemented data assimilation techniques with, in principle, any time-stepping model duscribing a process(atmospheric processes, 3D circulation, 2D water level, sea surface temperature, soil systems, groundwater etc.). Presently, OpenDA features filtering techniques and calibration techniques. The presentation will give an overview of the OpenDA and the results of some of its practical applications. Application of data assimilation in portable operational forecasting systems—the DATools assimilation environment, El Serafy G.Y., H. Gerritsen, S. Hummel, A. H. Weerts, A.E. Mynett and M. Tanaka (2007), Journal of Ocean Dynamics, DOI 10.1007/s10236-007-0124-3, pp.485-499. COSTA a problem solving environment for data assimilation applied for hydrodynamical modelling, Van Velzen and Verlaan (2007), Meteorologische Zeitschrift, Volume 16, Number 6, December 2007 , pp. 777-793(17). Application of generic data assimilation tools (DATools) for flood

  12. The Near-Earth Flyby of Asteroid 2012 DA14 (Invited)

    NASA Astrophysics Data System (ADS)

    Moskovitz, N. A.; Endicott, T.; Lister, T.; Ryan, B.; Ryan, E. V.; Willman, M.; Hergenrother, C.; Binzel, R. P.; Polishook, D.; DeMeo, F.; Bennechi, S.; Sheppard, S.; Marchis, F.; Angusteijn, T.; Birthwhistle, P.; Verveer, A.; Gulbis, A.; Nagayama, T.; Gilmore, A.; Kilmartin, P.

    2013-12-01

    On UT 2013 February 15, the elongated 20x40 meter near-Earth asteroid 2012 DA14 made a close flyby of the Earth at a distance of approximately 27,000 km or 4 Earth-radii. This passage was inside the orbital distance of geosynchronous satellites and close to the Earth's Roche limit. This close encounter was unprecedented in our advance knowledge of the incoming trajectory, thereby allowing us to forge a detailed coordinated plan for observation and testing of physical models predicting tidal effects near our planet's Roche limit. Predicted outcomes of such close encounters include tidal disruption, changes in rotation state, and mass loss or mass redistribution due to seismic shaking. Each of these effects sensitively depend on how tidal energy is dissipated in the interior of the asteroid: a rubble pile will dissipate tidal energy differently than a solid or partially fractured monolith. The internal properties of asteroids in general and particularly those in the size range of 2012 DA14 are largely unknown. This encounter represents one of the first ever opportunities to observationally test models of the interior structure of small planetary bodies. We will present the results of a large multi-observatory campaign focused on 2012 DA14. This campaign includes data from shortly after discovery in 2012 and surrounding the close approach of the asteroid in February of 2013. These data were collected with over a dozen telescopes worldwide, ranging in aperture from 1m to 8m and include major facilities in Chile, Australia, New Zealand, South Africa, Israel, Hawaii, Arizona, New Mexico, and the Canary Islands. We will highlight the outcome of pre- and post-flyby visible-wavelength photometric and spectroscopic observations. This will include clear evidence that 2012 DA14 is now in a non-principal axis rotation state, requiring a multi-periodic tumbling model to fit its post-flyby rotational light curve. Such a complex rotation state is consistent with models for the

  13. The Greek Database of Seismogenic Sources (GreDaSS): the new version.

    NASA Astrophysics Data System (ADS)

    Caputo, Riccardo; Pavlides, Spyros; GreDaSS Working Group

    2014-05-01

    The Greek Database of Seismogenic Sources (GreDaSS) is a repository of geological, tectonic and active fault data for the Greek territory and its surroundings including sectors of western Anatolia, southern Bulgaria, FYR of Macedonia and Albania. The principal aim of this international project is to create a homogenized framework of all data relevant to the seismotectonics, and especially the seismic hazard assessment, of Greece and its surroundings as well as a common research platform for performing seismic hazard analyses, modelling and scenarios from specific seismogenic structures. GreDaSS represents an on-going project based on the results of decades of investigations by the GreDaSS Working Group* and by numerous other researchers working on the active tectonics of the broader Aegean Region. Members of the WG have contributed in several national and European projects like FAUST (Faults as a seismological tool, 1998-2000) and SHARE (Seismic Hazard and Armonization in Europe, 2009-2012). The database is continuously updated by the WG, with the aim of improving its completeness in terms of seismogenic sources analysed and included, but also of enriching and better defining all parametric and ancillary information on each source. Preliminary and partial versions of GreDaSS have been already presented at previous congresses (Pavlides et al., 2010; Caputo et al., 2012; Sboras et al., 2013), but at next EGU the whole area and the most recent version of the database will be displayed. *) the principal scientific contributors belonging to the GreDaSS WG are: Sboras. S., Chatzipetros A., Koukouvelas I., Michailidou A., Valkaniotis S., Tarabusi G. Caputo R., Chatzipetros A., Pavlides S. and Sboras S. (2012): Ann. Geophysics, 55(5), 859-894, doi: 10.4401/ag-5168 Pavlides S., Caputo R., Sboras S., Chatzipetros A., Papathanasiou G. & Valkaniotis S. (2010): Bull. Geol. Soc. Greece, 43(1), 486-494 Sboras S., Pavlides S., Caputo R., Chatzipetros A., Michailidou A

  14. Rapid purification and direct microassay of calbindin9kDa utilizing its solubility in perchloric acid.

    PubMed Central

    Hubbard, M J

    1993-01-01

    The 9 kDa calcium-binding protein, calbindin9kDa, was found to be soluble in 7% (v/v) perchloric acid. Calbindin9kDa was easily purified from rat duodenum in 1 day with perchloric acid precipitation followed by reverse-phase h.p.l.c. The yield was 21.4 +/- 2.3 nmol/g wet weight of tissue (mean +/- S.E.M.; n = 3) from normally fed 7-8-week-old rats (approx. 70% recovery). The purification was also effective with rabbit duodenum calbindin9kDa, but not with various other EF-hand calcium-binding proteins tested in the rat. Several criteria (h.p.l.c., u.v. spectrum, denaturing two-dimensional PAGE, N-terminal sequencing) indicated that the rat calbindin9kDa was purified to homogeneity and was not affected by proteolysis. High-affinity calcium-binding properties were retained and no evidence of isoforms or charge modification was observed. Residue 59, identified as Asn (not Asp as previously reported), was fully amidated. When adopted as a microassay with isocratic h.p.l.c., the perchloric acid procedure enabled rapid (less than 6 min) and direct (peptide bond absorbance) quantification of less than 1 pmol of calbindin9kDa. This new approach to purification and assay will be of particular utility for investigations of calbindin9kDa in previously intractable low-abundance sources (e.g. cultured cells). Images Figure 1 Figure 2 PMID:8392333

  15. 15 kDa Granulysin versus GM-CSF for monocytes differentiation: analogies and differences at the transcriptome level

    PubMed Central

    2011-01-01

    Background Granulysin is an antimicrobial and proinflammatory protein with several isoforms. While the 9 kDa isoform is a well described cytolytic molecule with pro-inflammatory activity, the functions of the 15 kDa isoform is less well understood. Recently it was shown that 15 kDa Granulysin can act as an alarmin that is able to activate monocytes and immature dendritic cells. Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor widely used in immunotherapy both for in vivo and ex vivo applications, especially for its proliferative effects. Methods We analyzed gene expression profiles of monocytes cultured with 15 kDa Granulysin or GM-CSF for 4, 12, 24 and 48 hours to unravel both similarities and differences between the effects of these stimulators. Results The analysis revealed a common signature induced by both factors at each time point, but over time, a more specific signature for each factor became evident. At all time points, 15 kDa Granulysin induced immune response, chemotaxis and cell adhesion genes. In addition, only 15 kDa Granulsyin induced the activation of pathways related to fundamental dendritic cell functions, such as co-stimulation of T-cell activation and Th1 development. GM-CSF specifically down-regulated genes related to cell cycle arrest and the immune response. More specifically, cytokine production, lymphocyte mediated immunity and humoral immune response were down-regulated at late time points. Conclusion This study provides important insights on the effects of a novel agent, 15 kDa granulysin, that holds promise for therapeutic applications aimed at the activation of the immune response. PMID:21501511

  16. Synergetic effects of DA-6/GA₃ with EDTA on plant growth, extraction and detoxification of Cd by Lolium perenne.

    PubMed

    He, Shanying; Wu, Qiuling; He, Zhenli

    2014-12-01

    Research is needed to improve efficiency of phytoextraction of heavy metals from contaminated soils. A pot experiment was carried out to study the effects of plant growth regulators (PGRs) (diethyl aminoethyl hexanoate (C18H33NO8, DA-6) and gibberellic acid 3 (C19H22O6, GA3)) and/or EDTA on Cd extraction, subcellular distribution and chemical forms in Lolium perenne. The addition of EDTA or PGRs significantly enhanced Cd extraction efficiency (P<0.05), with the decreasing order of: 1 μM DA-6>10 μM DA-6>10 μM GA3>2.5 mmol kg(-1) EDTA>other treatments of PGR alone. PGRs+EDTA resulted in a further increase in Cd extraction efficiency, with EDTA+1 μM DA-6 being the most efficient. At the subcellular level, about 44-57% of Cd was soluble fraction, 18-44% in cell walls, and 12-25% in cellular organelles fraction. Chemical speciation analysis showed that 40-54% of Cd was NaCl extractable, 7-23% HAc extractable, followed by other fractions. EDTA increased the proportions of Cd in soluble and cellular organelles fraction, as well as the metal migration in shoot; therefore, the toxicity to plant increased and plant growth was inhibited. Conversely, PGRs fixed more Cd in cell walls and reduced Cd migration in shoot; thus, metal toxicity was reduced. In addition, PGRs promoted plant biomass growth significantly (P<0.05), with 1 μM DA-6 being the most effective. A combination of DA-6/GA3 with EDTA can alleviate the adverse effect of EDTA on plant growth, and the treatment of EDTA+1 μM DA-6 appears to be optimal for improving the remediation efficiency of L. perenne for Cd contaminated soil. PMID:24999226

  17. Sequence and expression of an Eisenia-fetida-derived cDNA clone that encodes the 40-kDa fetidin antibacterial protein.

    PubMed

    Lassegues, M; Milochau, A; Doignon, F; Du Pasquier, L; Valembois, P

    1997-06-15

    Fetidins are 40-kDa and 45-kDa hemolytic and antibacterial glycoproteins present in the coelomic fluid of the earthworm Eisenia fetida andrei. By screening a cDNA library with a polyclonal antifetidin serum, we have cloned a cDNA that encoded the 40-kDa fetidin. The clone contains an insert of 1.44 kb encoding a protein of 34 kDa, which corresponds to the size of deglycosylated fetidins. The recombinant protein inhibits Bacillus megaterium growth. Restriction fragment polymorphisms were observed on Southern blots and correspond to a known protein polymorphism. The sequence of the cDNA contains a peroxidase signature and fetidins from earthworm coelomic fluid have peroxidase activity. The 40-kDa and 45-kDa fetidins therefore represent two related polymorphic defence factors in invertebrates. PMID:9219536

  18. 11 CFR 111.15 - Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 11 Federal Elections 1 2012-01-01 2012-01-01 false Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). 111.15 Section 111.15 Federal Elections FEDERAL ELECTION COMMISSION GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111.15 Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). (a) Any person...

  19. 11 CFR 111.15 - Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 11 Federal Elections 1 2013-01-01 2012-01-01 true Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). 111.15 Section 111.15 Federal Elections FEDERAL ELECTION COMMISSION GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111.15 Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). (a) Any person...

  20. Evolution of DA white dwarfs in the context of a new theory of convection

    NASA Astrophysics Data System (ADS)

    Althaus, L. G.; Benvenuto, O. G.

    1998-05-01

    In this study we compute the structure and evolution of carbon-oxygen DA (hydrogen-rich envelope) white dwarf models by means of a detailed and updated evolutionary code. We consider models with masses from 0.5 to 1.0Msolar and we vary the hydrogen layer mass in the interval 10^-13<=M_H/M≮10^-4. In particular, we treat the energy transport by convection within the formalism of the full-spectrum turbulence theory, as given by the Canuto, Goldman & Mazzitelli (CGM) model. We explore the effect of various hydrogen layer masses on both the surface gravity and the hydrogen burning. Convective mixing at low luminosities is also considered. One of our main interests in this work has been to study the evolution of ZZ Ceti models, with the aim of comparing the CGM and mixing-length theory (MLT) predictions. In this connection, we find that the temperature profile given by the CGM model is markedly different from that of the ML1 and ML2 versions of the MLT. In addition, the evolving outer convection zone behaves differently in both theories. We have also computed approximate effective temperatures for the theoretical blue edge of the DA instability strip by using thermal time-scale arguments for our evolving DA models. In this context, we found that the CGM theory leads to blue edges that are cooler than the observed ones. However, because the determination of atmospheric parameters of ZZ Ceti stars is dependent on the assumed convection description in model atmosphere calculations, observed blue edges based on model atmospheres computed considering the CGM theory are required in order to perform a self-consistent comparison of our results with observations. Finally, detailed non-adiabatic pulsational computations of ZZ Ceti models considering the CGM convection would be necessary to place the results found in this paper on a firmer basis.

  1. [The Vitruvian Man: an anatomical drawing for proportions by Leonardo Da Vinci].

    PubMed

    Le Floch-Prigent, P

    2008-12-01

    The aim of the study was to find out and to analyse the text by Vitruvius which inspired the famous drawing by Leonardo da Vinci (circa 1490) kept in the Galleria dell'Accademia, in Venezia, Italy: the man inscribed in one circle and in one square. The book "de Architectura" by Vitruvius Marcus Pollio was printed several times since the Renaissance when both the roman architecture of antiquity and this text became very popular. From a French translation by Claude Perrault in 1864, it became easy to find a French translation with the original text in Latin (Paris, 2003, Les Belles Lettres, French text by Pierre Gros). The drawing by Leonardo da Vinci illustrates with great accuracy and fidelity the quotation of Vitruvius (with the exception of two of the 12 main relationships). The genius of Leonardo da Vinci was to keep only one trunk, head and neck for two pairs of limbs: scapular and pelvic; to make the circle tangent to the lower edge of the square; to adjust a few features of the quotation for the equilibrium of the whole figure; and of course to bring his incredible skill as a drawer (one of the best of his century). The drawing was made on a sheet of paper 344x245mm, in black ink which became dark brown with time; several lines complete the figure above and below; a short caption and a horizontal scale appear just under the drawing. The celebrity of the drawing, a symbol of the Renaissance, of the equilibrium of man and mankind, of the universality of the artists and intellectuals of the time (Humanism) made it iconic and it has been constantly reproduced and adapted especially for advertisement and logos, not only in the medical field. PMID:18951824

  2. Structural requirements to obtain highly potent and selective 18 kDa Translocator Protein (TSPO) Ligands.

    PubMed

    Taliani, Sabrina; Pugliesi, Isabella; Da Settimo, Federico

    2011-01-01

    The (18 kDa) Translocator Protein (TSPO), was initially identified in 1977 as peripheral binding site for the benzodiazepine diazepam and named "Peripheral-type benzodiazepine receptor (PBR)". It is an evolutionarily well-conserved protein particularly located at the outer/inner mitochondrial membrane contact sites, in closely association with the 32 kDa voltage-dependent anion channel (VDAC) and the 30 kDa adenine nucleotide translocase (ANT), thus forming the mitochondrial permeability transition pore (MPTP). TSPO is ubiquitary expressed in peripheral tissues (steroid producing tissues, liver, heart, kidney, lung, immune system) and in lower levels in the central nervous system, where it is mainly located in glial cells, and in neurons. TSPO is involved in a variety of biological processes such as cholesterol transport, steroidogenesis, calcium homeostasis, lipid metabolism, mitochondrial oxidation, cell growth and differentiation, apoptosis induction, and regulation of immune functions. In the last decade, many studies have reported that TSPO basal expression is altered in a number of human pathologies, such as cancer and neurodegenerative disorders (Huntington's and Alzheimer's diseases), as well as in various forms of brain injury and inflammation and anxiety. Consequently, TSPO has not only been suggested as a promising drug target for a number of therapeutic applications (anticonvulsant, anxiolytic, immunomodulating, etc.), but also as valid diagnostic marker for related-disease state and progression, prompting the development of specific labelled ligands as powerful tools for imaging techniques. A number of structurally different classes of ligands have been reported, showing high affinity and selectivity towards TSPO. Indeed, most of these ligands have been designed starting from selective CBR ligands which were structurally modified in order to shift their affinity towards TSPO. Extensive structure-activity relationship studies were performed allowing to

  3. Análise da medição do raio solar em ultravioleta

    NASA Astrophysics Data System (ADS)

    Saraiva, A. C. V.; Giménez de Castro, C. G.; Costa, J. E. R.; Selhorst, C. L.; Simões, P. J. A.

    2003-08-01

    A medição acurada do raio solar em qualquer banda do espectro eletromagnético é de relevância na formulação e calibração de modelos da estrutura e atmosfera solar. Esses modelos atribuem emissão do contínuo do Sol calmo em microondas à mesma região da linha Ha do Hell. Apresentamos a medição do raio solar em UV com imagens do EIT (Extreme Ultraviolet Image Telescope) entre 1996 e 2002, no comprimento de onda 30,9 nm (Ha do Hell), que se forma na região de transição/cromosfera solar. A técnica utilizada para o cálculo do raio UV foi baseada na transformada Wavelet B3spline. Fizemos um banco de dados com 1 imagem por dia durante o período citado. Obtivemos como resultado o raio médio da ordem de 975.61" e uma diminuição do mesmo para o período citado variando em média -0,45" /ano. Comparamos estes dados com os valores obtidos pelo ROI (Radio Observatório de Itapetinga) em 22/48 GHz e Nobeyama Radio Heliograph em 17 GHz mostrando que os raios médios são muito próximos o que indica que a região de formação nessas freqüências é a mesma conforme os modelos. Comparamos os resultados também com outros índices de atividade solar.

  4. Crystal structure of the 500-kDa yeast acetyl-CoA carboxylase holoenzyme dimer.

    PubMed

    Wei, Jia; Tong, Liang

    2015-10-29

    Acetyl-CoA carboxylase (ACC) has crucial roles in fatty acid metabolism and is an attractive target for drug discovery against diabetes, cancer and other diseases. Saccharomyces cerevisiae ACC (ScACC) is crucial for the production of very-long-chain fatty acids and the maintenance of the nuclear envelope. ACC contains biotin carboxylase (BC) and carboxyltransferase (CT) activities, and its biotin is linked covalently to the biotin carboxyl carrier protein (BCCP). Most eukaryotic ACCs are 250-kilodalton (kDa), multi-domain enzymes and function as homodimers and higher oligomers. They contain a unique, 80-kDa central region that shares no homology with other proteins. Although the structures of the BC, CT and BCCP domains and other biotin-dependent carboxylase holoenzymes are known, there is currently no structural information on the ACC holoenzyme. Here we report the crystal structure of the full-length, 500-kDa holoenzyme dimer of ScACC. The structure is remarkably different from that of the other biotin-dependent carboxylases. The central region contains five domains and is important for positioning the BC and CT domains for catalysis. The structure unexpectedly reveals a dimer of the BC domain and extensive conformational differences compared to the structure of the BC domain alone, which is a monomer. These structural changes reveal why the BC domain alone is catalytically inactive and define the molecular mechanism for the inhibition of eukaryotic ACC by the natural product soraphen A and by phosphorylation of a Ser residue just before the BC domain core in mammalian ACC. The BC and CT active sites are separated by 80 Å, and the entire BCCP domain must translocate during catalysis. PMID:26458104

  5. Quadrupole Ion Mass Spectrometer for Masses of 2 to 50 Da

    NASA Technical Reports Server (NTRS)

    Helms, William; Griffin, Timothy P.; Ottens, Andrew; Harrison, Willard

    2005-01-01

    A customized quadrupole ion-trap mass spectrometer (QITMS) has been built to satisfy a need for a compact, rugged instrument for measuring small concentrations of hydrogen, helium, oxygen, and argon in a nitrogen atmosphere. This QITMS can also be used to perform quantitative analyses of other gases within its molecular-mass range, which is 2 to 50 daltons (Da). (More precisely, it can be used to perform quantitative analysis of gases that, when ionized, are characterized by m/Z ratios between 2 and 50, where m is the mass of an ion in daltons and Z is the number of fundamental electric charges on the ion.

  6. The strainrange partitioning behavior of an advanced gas turbine disk alloy, AF2-1DA

    NASA Technical Reports Server (NTRS)

    Halford, G. R.; Nachtigall, A. J.

    1979-01-01

    The low-cycle, creep-fatigue characteristics of the advanced gas turbine disk alloy, AF2-1DA have been determined at 1400 F and are presented in terms of the method of strainrange partitioning (SRP). The mean stresses which develop in the PC and CP type SRP cycles at the lowest inelastic strainrange were observed to influence the cyclic lives to a greater extent than the creep effects and hence interfered with a conventional interpretation of the results by SRP. A procedure is proposed for dealing with the mean stress effects on life which is compatible with SRP.

  7. Study of the hadronic interactions of kaons in light nuclei at DA{Phi}ne

    SciTech Connect

    Vazquez Doce, O.

    2010-08-05

    The AMADEUS experiment at the Da{Phi}ne accelerator of the Frascati National Laboratories (Italy) of INFN, will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, with a complete experimental program for the case of the kaonic clusters. Studying the absorption of antikaon by the nucleus will provide information concerning the K-barN interaction and the modification of the kaon mass in the nuclear medium.A preliminar study of these kind of hadronic interactions is being done by the AMADEUS collaboration by analyzing the existent KLOE data.

  8. The magmatic source of the Tristan da Cunha hotspot: Implication from electrical conductivity

    NASA Astrophysics Data System (ADS)

    Chen, Jin; Baba, Kiyoshi; Jegen, Marion; Utada, Hisashi; Geissler, Wolfram; Jokat, Wilfried

    2016-04-01

    Tristan da Cunha Island is one of the classical hot spots in the Atlantic Ocean, situated at the western end of the aseismic Walvis Ridge which forms a connection to the Cretaceous Etendeka flood basalt province in northwestern Namibia. The discussion about its source (in shallow asthenosphere or deeper mantle) have not reached consensus yet because of lack of the geophysical observations in the area. A marine magnetotelluric (MT) experiment was conducted together with seismological observations in the area in 2012-2013 through a German-Japanese collaboration with the goal to constrain the physical state of the mantle beneath the area. A total of 26 MT seafloor stations were deployed around the Tristan da Cunha Islands and available data were retrieved and processed from 24 stations. We applied iterative topographic effect correction and one-dimensional (1-D) conductivity structure inversion to the data. Then, three-dimensional (3-D) inversion analysis incorporating the topographic effect was carried out, using the 1-D model as the initial model. The local small-scale topography and the far continental coast effects are incorporated as the distortion term in the 3-D inversion. The preliminary result of our analysis shows no evidence of a significant conductive anomaly arising from the mantle transition zone, suggesting that the current magmatic source (major place of melting) of the hotspot activity is in the shallow upper mantle. This is in contrast to results from geochemical analysis, in which samples along the Tristan track exhibit an ocean-island-basalt-type incompatible element pattern pointing to a deep mantle source of the melt. Our findings therefore might indicate that the deep mantle up-welling underneath Tristan da Cunha Islands may be almost dead. A conductive anomaly at approx. 100 km depth in our derived conductivity model to the southwest of Tristan da Cunha Islands suggests an interaction between the mid-ocean ridge and/or up-welling further south

  9. Middle Paleolithic human remains from the Gruta da Oliveira (Torres Novas), Portugal.

    PubMed

    Trinkaus, Erik; Maki, Julia; Zilhão, João

    2007-10-01

    Ongoing excavations in the Middle Paleolithic levels at the Gruta da Oliveira, Portugal have yielded four fragmentary human remains, a manual phalanx and an ulna from levels 9 and 10, and a humerus and a tibia from levels 18 and 19. The first two remains date to approximately 39 ka 14C BP ( approximately 43.5 ka cal BP), whereas the latter two derive from earlier in oxygen isotope stage 3. The preserved portions of the phalanx, humerus, and tibia align them morphologically with the Neandertals. In addition, the Oliveira 4 tibial diaphysis shows evidence of carnivore (probably canid) gnawing. PMID:17632802

  10. Implementação de um algoritmo para a limpeza de mapas da RCFM

    NASA Astrophysics Data System (ADS)

    Souza, C. L.; Wuensche, C. A.

    2003-08-01

    A Radiação Cósmica de Fundo em Microondas (RCFM), descoberta por Penzias e Wilson em 1965, é uma das ferramentas mais poderosas para o estudo da cosmologia. Com a descoberta de flutuações de temperatura na RCFM, da ordem de uma parte em 105, pelo COBE (1992), uma nova era teve início. Nos últimos onze anos, diversos instrumentos fizeram novas medidas de alta precisão, refinando os resultados apresentados pelo COBE, culminando com os resultados recentes do satélite WMAP. A análise de dados da RCFM, especialmente no caso de experimentos com pequena cobertura do céu, apresenta uma série de dificuldades devido a emissões de contaminantes externos, tais como a emissão da Galáxia e de fontes pontuais, e de ruídos intrínsecos tanto ao sistema de detecção quanto à estratégia de observação do céu. Uma das soluções típicas para a filtragem de dados brutos de um experimento para medir flutuações de temperatura é aplicar um gabarito (template) e um filtro passa alta ao produzir mapas simplificados (sem considerar matrizes de correlação ou covariância). No caso de experimentos que utilizam detectores HEMT, essa combinação de filtros remove, satisfatoriamente, ruídos do tipo 1/f gerados pela instabilidade no ganho do detector acoplado ao movimento do instrumento, definido pela estratégia de observação. Entretanto, o sinal resultante medido, tanto em simulações quanto em séries temporais reais, sugere que parte do sinal cosmológico pode estar sendo removido junto com o ruído dos detectores. Este trabalho descreve as etapas para a produção de um mapa típico (simulado) e os testes preliminares de um algoritmo para remover ruídos do tipo 1/f introduzidos pela estratégia de observação sem prejudicar a qualidade do sinal cosmológico presente no mapa.

  11. [Examples of textual research on gynecological literature included in Yong le da dian (Yongle's Great Canon)].

    PubMed

    Zhang, Xue-dan; Zhang, Ru-qing

    2010-07-01

    Yongle's Great Canon is a series of books to be read exclusively by the emperors of the Ming Dynasty, so all of the books included are from many rare books collected in Wen Yuan Ge of early Ming Dynasty. These rare books, ranging from Song, Jin, and Yuan Dynasties or from folk sources preserve the original appearance of the ancient books almost without any changes. From a philological viewpoint, the gynecological books included in Yong le da dian are studied, proof-read and checked for its lost texts and different texts. This can serve as a basis for the reprinting and re-publishing of ancient TCM books. PMID:21122346

  12. Prospective EUV observations of hot DA white dwarfs with the EUV Explorer

    NASA Technical Reports Server (NTRS)

    Finley, David S.; Malina, Roger F.; Bowyer, Stuart

    1987-01-01

    The Extreme Ultraviolet Explorer (EUVE) will perform a high sensitivity EUV all-sky survey. A major category of sources which will be detected with the EUVE instruments consists of hot white dwarfs. Detailed preliminary studies of synthetic EUV observations of white dwarfs have been carried out using the predicted EUVE instrumental response functions. Using available information regarding space densities of white dwarfs and the distribution of neutral hydrogen in the interstellar medium, the numbers of DA white dwarfs which will be detectable in the different EUV bandpasses have been estimated.

  13. Molluscan mega-hemocyanin: an ancient oxygen carrier tuned by a ~550 kDa polypeptide

    PubMed Central

    2010-01-01

    Background The allosteric respiratory protein hemocyanin occurs in gastropods as tubular di-, tri- and multimers of a 35 × 18 nm, ring-like decamer with a collar complex at one opening. The decamer comprises five subunit dimers. The subunit, a 400 kDa polypeptide, is a concatenation of eight paralogous functional units. Their exact topology within the quaternary structure has recently been solved by 3D electron microscopy, providing a molecular model of an entire didecamer (two conjoined decamers). Here we study keyhole limpet hemocyanin (KLH2) tridecamers to unravel the exact association mode of the third decamer. Moreover, we introduce and describe a more complex type of hemocyanin tridecamer discovered in fresh/brackish-water cerithioid snails (Leptoxis, Melanoides, Terebralia). Results The "typical" KLH2 tridecamer is partially hollow, whereas the cerithioid tridecamer is almost completely filled with material; it was therefore termed "mega-hemocyanin". In both types, the staggering angle between adjoining decamers is 36°. The cerithioid tridecamer comprises two typical decamers based on the canonical 400 kDa subunit, flanking a central "mega-decamer" composed of ten unique ~550 kDa subunits. The additional ~150 kDa per subunit substantially enlarge the internal collar complex. Preliminary oxygen binding measurements indicate a moderate hemocyanin oxygen affinity in Leptoxis (p50 ~9 mmHg), and a very high affinity in Melanoides (~3 mmHg) and Terebralia (~2 mmHg). Species-specific and individual variation in the proportions of the two subunit types was also observed, leading to differences in the oligomeric states found in the hemolymph. Conclusions In cerithioid hemocyanin tridecamers ("mega-hemocyanin") the collar complex of the central decamer is substantially enlarged and modified. The preliminary O2 binding curves indicate that there are species-specific functional differences in the cerithioid mega-hemocyanins which might reflect different physiological

  14. [Science and nation: romanticism and natural history in the works of E. J. da Silva Maia].

    PubMed

    Kury, L

    1998-01-01

    The works of physician and naturalist Emílio Joaquim da Silva Maia (1808-59) can be viewed as a scientific project that discovers Brazil and its inhabitants. Maia's nationalism and his romantic view of nature formed the underpinnings of his scientific theories, especially his studies on zoological geography. He subordinated the issue of the biological specificity of different regions of the world to his era's debates on the construction of Brazil as an independent nation. In his interpretations of European natural history, Maia endeavored to understand Brazilian nature as a specific achievement of the Cosmos, in keeping with Alexander von Humboldt's approach. PMID:16676427

  15. The discontinuity near 1600 A in the spectra of DA white dwarfs

    NASA Technical Reports Server (NTRS)

    Wegner, G.

    1984-01-01

    Ultraviolet spectroscopic observations of two relatively cool DA white dwarfs, L481 - 60 (= WD 1544 - 37) and BPM 1266 ( = WD 2105 - 82), with the International Ultraviolet Explorer (IUE) satellite show a strong drop in their spectral energy distributions below 1600 A. Published model atmospheres and thier visual spectra suggest that these two stars have effective temperatures in the vicinity of 9,000-10,000 K, and it is proposed that the 1600 A feature could be due to the 342(1S) 3s2(1S) photoionization edge of Mg I.

  16. OCT structural examination of Madonna dei Fusi by Leonardo da Vinci

    NASA Astrophysics Data System (ADS)

    Targowski, Piotr; Iwanicka, Magdalena; Sylwestrzak, Marcin; Kaszewska, Ewa A.; Frosinini, Cecilia

    2013-05-01

    Madonna dei Fusi (`Madonna of the Yarnwider') is a spectacular example of Italian Renaissance painting, attributed to Leonardo da Vinci. The aim of this study is to give an account of past restoration procedures. The evidence of a former retouching campaign will be presented with cross-sectional images obtained non-invasively with Optical Coherence Tomography (OCT). Specifically, the locations of overpaintings/retouchings with respect to the original paint layer and secondary varnishes will be given. Additionally, the evidence of a former transfer of the pictorial layer to the new canvas support by detecting the presence of its structure incised into paint layer will be shown.

  17. Pietro Anzolino da Eboli and the thermal therapy of renal pathologies.

    PubMed

    Mezzogiorno, Antonio; Caruso, Arturo Armone; Lorio, Luigi; Papa, Michele; Esposito, Vincenzo

    2004-01-01

    In the Campi Flegrei area of Campania, a region of Southern Italy, some thermal baths still exist, known for the therapeutical properties of their waters. Utilized since remote ages for the treatment of a variety of pathologies. In "De Balneis Terrae Laboris", an Italian poet of the Middle Ages and scientist, Pietro Anzolino da Eboli, reports every detail about more than thirty different thermal sites in the Campi Flegrei, including all therapeutic effects of their waters. Based on Pietro's manuscript, we report in the current paper all the sites whose waters were - among other indications - also recommended for the treatment of some urinary diseases. PMID:15293539

  18. Purification and characterization of a novel 88 kDa protein from serum and vitreous of patients with Eales' disease.

    PubMed

    Sulochana, K N; Rajesh, M; Ramakrishnan, S

    2001-10-01

    Eales' disease is a perivasculitis that affects the peripheral retina of young adults and results in recurrent vitreous hemorrhage. Although increased oxidative stress and decreased antioxidant defense have been reported to be associated with Eales' disease, the exact cause for the disease and its pathogenesis are not known. Here is reported the identification, purification and characterization of a new protein from the serum and vitreous of patients with Eales' disease. This protein was purified using preparative electrophoresis and HPLC. The purified protein had a retention time of 9.2 min in RP HPLC. Its molecular weight as determined by gel permeation chromatography was 88 kDa hence, it was termed as 88 kDa protein. Alcian blue and Schiffs staining revealed 88 kDa protein to be a glycoprotein. Proteins purified from both serum and vitreous exhibited anti lipid peroxidation effect on erythrocyte when added during in vitro assay of thiobarbuteric acid reactive substances (TBARS). In addition to this property the protein also has Fe(2+)sequestering effect. The anti TBARS activity of 88 kDa protein was completely inhibited by 0.1 m M concentration of parachlromercuric benzoate (PCMB) and 5,5' dithiobis(2-nitrobenzoic acid) DTNB. The total thiol content (cysteine) of the purified 88 kDa protein was found to be 8% by mass. Eighty eight kDa protein from both the sources namely vitreous and serum are immunologically identical when studied using polyclonal antibodies raised in goat against purified serum protein. The N terminal sequence of 88 kDa protein by automated Edman's degradation chemistry is A D D P N S L S P S A F A E A L A L L R D S X L A R F V. The protein and DNA data base search revealed no match to 88 kDa protein and hence this was considered as unique protein. Further knowledge on the in vivo function of 88 kDa protein is very important to understand its role in the pathogenesis of Eales' disease. PMID:11825025

  19. Circulating non-22 kDa growth hormone isoforms after a repeated GHRH stimulus in normal subjects.

    PubMed

    Coya, R; Algorta, J; Boguszewski, C L; Vela, A; Carlsson, L M S; Aniel-Quiroga, A; Busturia, M A; Martul, P

    2005-04-01

    The aim of this study was to evaluate the proportion of non-22 kDa GH isoforms in relation to total GH concentration after a repeated GHRH stimulus in healthy subjects. We studied 25 normal volunteers (12 males and 13 females, mean age 13.1 years, range 6-35), who received two GHRH bolus (1.5 mug/kg body weight, i.v.) administered separately by an interval of 120 minutes. The proportion of non-22 kDa GH was determined by the 22 kDa GH exclusion assay (GHEA), which is based on immunomagnetic extraction of monomeric and dimeric 22 kDa GH from serum, and quantitation of non-22 kDa GH isoforms using a polyclonal GH assay. Samples were collected at baseline and at 15-30 min intervals up to 240 min for total GH concentration. Non-22 kDa GH isoforms were measured in samples where peak GH after GHRH was observed. Total GH peaked after the first GHRH bolus in all subjects (median 37.2 ng/ml; range: 10.4-94.6). According to GH response to the second GHRH stimulus, the study group was divided in "non-responders" (n=7; 28%), with GH peak levels lower than 10 ng/ml (median GH: 8.7 ng/ml; range 7.3-9.6) and "responders" (n=18; 72%), who showed a GH response greater than 10 ng/ml (median 17 ng/ml; range 10.1-47.0). The median proportion of non-22 kDa GH on the peak of GH secretion after the first GHRH administration was similar in both groups ("responders" median: 8.6%, range 7-10.9%; "non-responders" median: 8.7%, range 6.7-10.3%), independently of the type of response after the second GHRH. In contrast, the median proportion of non-22 kDa GH was greater at time of GH peak after the second GHRH bolus in the "non-responders" (median 11.4%; range 9.1-14.3%) in comparison with the "responders" (median 9.1%; range 6.7-11.9%; p=0.003). A significant negative correlation between the total GH secreted and the percentage of non-22 kDa isoforms was seen in the "non-responders" (p=0.003). These differences in GH response to repeated GHRH stimulation and in the pattern of GH isoforms at GH

  20. Da0324, an inhibitor of nuclear factor-κB activation, demonstrates selective antitumor activity on human gastric cancer cells

    PubMed Central

    Jin, Rong; Xia, Yiqun; Chen, Qiuxiang; Li, Wulan; Chen, Dahui; Ye, Hui; Zhao, Chengguang; Du, Xiaojing; Shi, Dengjian; Wu, Jianzhang; Liang, Guang

    2016-01-01

    Background The transcription factor nuclear factor-κB (NF-κB) is constitutively activated in a variety of human cancers, including gastric cancer. NF-κB inhibitors that selectively kill cancer cells are urgently needed for cancer treatment. Curcumin is a potent inhibitor of NF-κB activation. Unfortunately, the therapeutic potential of curcumin is limited by its relatively low potency and poor cellular bioavailability. In this study, we presented a novel NF-κB inhibitor named Da0324, a synthetic asymmetric mono-carbonyl analog of curcumin. The purpose of this study is to research the expression of NF-κB in gastric cancer and the antitumor activity and mechanism of Da0324 on human gastric cancer cells. Methods The expressions between gastric cancer tissues/cells and normal gastric tissues/cells of NF-κB were evaluated by Western blot. The inhibition viability of compounds on human gastric cancer cell lines SGC-7901, BGC-823, MGC-803, and normal gastric mucosa epithelial cell line GES-1 was assessed with the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Absorption spectrum method and high-performance liquid chromatography method detected the stability of the compound in vitro. The compound-induced changes of inducible NF-κB activation in the SGC-7901 and BGC-823 cells were examined by Western blot analysis and immunofluorescence methods. The antitumor activity of compound was performed by clonogenic assay, matrigel invasion assay, flow cytometric analysis, Western blot analysis, and Hoechst 33258 staining assay. Results High levels of p65 were found in gastric cancer tissues and cells. Da0324 displayed higher growth inhibition against several types of gastric cancer cell lines and showed relatively low toxicity to GES-1. Moreover, Da0324 was more stable than curcumin in vitro. Western blot analysis and immunofluorescence methods showed that Da0324 blocked NF-κB activation. In addition, Da0324 significantly inhibited tumor proliferation

  1. The Ubiquitin Receptor DA1 Regulates Seed and Organ Size by Modulating the Stability of the Ubiquitin-Specific Protease UBP15/SOD2 in Arabidopsis[W

    PubMed Central

    Du, Liang; Li, Na; Chen, Liangliang; Xu, Yingxiu; Li, Yu; Zhang, Yueying; Li, Chuanyou; Li, Yunhai

    2014-01-01

    Although the control of organ size is a fundamental question in developmental biology, little is known about the genetic and molecular mechanisms that determine the final size of seeds in plants. We previously demonstrated that the ubiquitin receptor DA1 acts synergistically with the E3 ubiquitin ligases DA2 and ENHANCER1 OF DA1 (EOD1)/BIG BROTHER to restrict seed growth in Arabidopsis thaliana. Here, we describe UBIQUITIN-SPECIFIC PROTEASE15 (UBP15), encoded by SUPPRESSOR2 OF DA1 (SOD2), which acts maternally to regulate seed size by promoting cell proliferation in the integuments of ovules and developing seeds. The sod2/ubp15 mutants form small seeds, while overexpression of UBP15 increases seed size of wild-type plants. Genetic analyses indicate that UBP15 functions antagonistically in a common pathway with DA1 to influence seed size, but does so independently of DA2 and EOD1. Further results reveal that DA1 physically associates with UBP15 in vitro and in vivo and modulates the stability of UBP15. Therefore, our findings establish a genetic and molecular framework for the regulation of seed size by four ubiquitin-related proteins DA1, DA2, EOD1, and UBP15 and suggest that they are promising targets for increasing seed size in crops. PMID:24585836

  2. Increased susceptibility of ethanol-treated gastric mucosa to naproxen and its inhibition by DA-9601, an Artemisia asiatica extract

    PubMed Central

    Oh, Tae Young; Ahn, Gook Jun; Choi, Seul Min; Ahn, Byoung Ok; Kim, Won Bae

    2005-01-01

    AIM: To examine the effect of DA-9601, a new gastroprotective agent, on the vulnerability of ethanol-treated rat’s stomach to naproxen (NAP). METHODS: Male Sprague-Dawley rats were pretreated with 1 mL of 50% ethanol twice a day for 5 d and then NAP (50 mg/kg) was administered. DA-9601 was administered 1 h before NAP. Four hours after NAP, the rats were killed to examine gross injury index (mm2), histologic change and to determine mucosal levels of malondialdehyde (MDA), prostaglandin E2 (PGE2), glutathione (GSH) and myeloperoxidase (MPO). RESULTS: Pretreatment of ethanol significantly increased NAP-induced gastric lesions, as well as an increase in MDA and MPO. On the contrary, mucosal PGE2 and GSH contents were decreased dramatically by ethanol pretreatment, which were aggravated by NAP. DA-9601 significantly reduced NAP-induced gastric injury grossly and microscopically, regardless of pretreatment with ethanol. DA-9601 preserved, or rather, increased mucosal PGE2 and GSH in NAP-treated rats (P<0.05), with reduction in mucosal MDA and MPO levels. CONCLUSION: These results suggest that repeated alcohol consumption renders gastric mucosa more susceptible to NSAIDs though, at least in part, reduction of endogenous cytoprotectants including PGE2 and GSH, and increase in MPO activation, and that DA-9601, a new gastroprotectant, can reduce the increased vulnerability of ethanol consumers to NSAIDs-induced gastric damage via the mechanism in which PGE2 and GSH are involved. PMID:16437715

  3. Evaluating the potential of marine Bacteriovorax sp. DA5 as a biocontrol agent against vibriosis in Litopenaeus vannamei larvae.

    PubMed

    Wen, Chongqing; Xue, Ming; Liang, Huafang; Zhou, Shining

    2014-09-17

    The potential application of Bdellovibrio-and-like organisms (BALOs) for the biocontrol of bacterial diseases has been widely recognized. However, no marine BALOs have been reported for Vibrio-related infections in penaeid shrimp. In the present study, the bacteriolytic ability of the marine Bacteriovorax strain DA5 against Vibrio alginolyticus zouA was examined by cocultivation and electron microscopy, and optimal lysis was observed at 30-35°C and 20-30‰ salinity along with a high multiplicity of infection. Then, we showed that experimentally infected Litopenaeus vannamei larvae exhibited significantly higher survival with incremental DA5 levels. Finally, variation in the bacterial counts and the bacterial community in larval rearing water was investigated after prophylactic application of DA5. The elimination effect of DA5 on vibrios was visible at early time points, whereas only a few non-dominant bacteria, rather than the predominant populations, were affected through analysis of denaturing gradient gel electrophoresis of the 16S rDNA V3 region. Accordingly, the prophylactic and therapeutic efficacies of DA5 on vibriosis associated with L. vannamei could markedly enhance larval survivability, and these results will facilitate the application of marine Bacteriovorax to control vibriosis in shrimp larviculture. PMID:25139659

  4. Autoradiographic localization of dopamine DA1 receptors in rat kidney with ( sup 3 H)Sch 23390

    SciTech Connect

    Huo, T.; Healy, D.P. )

    1989-09-01

    Dopamine receptors in the rat kidney were characterized by homogenate binding and in vitro autoradiography using the dopamine 1 (DA1)-selective antagonist ({sup 3}H)Sch 23390. ({sup 3}H)Sch 23390 binding in cortical membrane preparations was saturable, stereoselective, and competed for by DA agonists and antagonists with a rank order of potency consistent with the labeling of the DA1 receptor. ({sup 3}H)Sch 22390 binding was best fit to a two-site model: a high affinity-low density site (KD1 4.9 +/- 1.4 nM, Bmax 1 31.4 +/- 13.8 fmol/mg protein) and a low affinity-high density site (KD2 86.4 +/- 23.9 nM, Bmax 2 848.0 +/- 227.4 fmol/mg protein). In vitro autoradiography indicated that ({sup 3}H)Sch 22390 binding sites were restricted to the cortex. High-resolution autoradiography further indicated that ({sup 3}H)Sch 22390 binding sites were localized primarily on proximal tubules. Glomeruli and other vascular elements were devoid of ({sup 3}H)Sch 23390 binding sites. These results suggest that DA and DA1 agonists may affect sodium excretion by a direct action on proximal tubule sodium reabsorption.

  5. Agarose and Polyacrylamide Gel Electrophoresis Methods for Molecular Mass Analysis of 5–500 kDa Hyaluronan

    PubMed Central

    Bhilocha, Shardul; Amin, Ripal; Pandya, Monika; Yuan, Han; Tank, Mihir; LoBello, Jaclyn; Shytuhina, Anastasia; Wang, Wenlan; Wisniewski, Hans-Georg; de la Motte, Carol; Cowman, Mary K.

    2011-01-01

    Agarose and polyacrylamide gel electrophoresis systems for the molecular mass-dependent separation of hyaluronan (HA) in the size range of approximately 5–500 kDa have been investigated. For agarose-based systems, the suitability of different agarose types, agarose concentrations, and buffers systems were determined. Using chemoenzymatically synthesized HA standards of low polydispersity, the molecular mass range was determined for each gel composition, over which the relationship between HA mobility and logarithm of the molecular mass was linear. Excellent linear calibration was obtained for HA molecular mass as low as approximately 9 kDa in agarose gels. For higher resolution separation, and for extension to molecular masses as low as approximately 5 kDa, gradient polyacrylamide gels were superior. Densitometric scanning of stained gels allowed analysis of the range of molecular masses present in a sample, and calculation of weight-average and number-average values. The methods were validated for polydisperse HA samples with viscosity-average molecular masses of 112, 59, 37, and 22 kDa, at sample loads of 0.5 µg (for polyacrylamide) to 2.5 µg (for agarose). Use of the methods for electrophoretic mobility shift assays was demonstrated for binding of the HA-binding region of aggrecan (recombinant human aggrecan G1-IGD-G2 domains) to a 150 kDa HA standard. PMID:21684248

  6. A estabilidade dos PAHS em função da energia da radiação interestelar nas faixas UV e raios-X

    NASA Astrophysics Data System (ADS)

    Pinotti, R.; Costa, R. K.; Boechat-Roberty, H. M.; Lago, A.; Souza, G. B.

    2003-08-01

    A nebulosa CRL 618, uma proto-nebulosa planetária cuja nuvem molecular espessa envolve uma estrela B0, contém uma grande quantidade de C2H2 e CH4. Estas moléculas são consideradas os tijolos da criação de grandes moléculas carbonadas como os Hidrocarbonetos Policíclicos Aromáticos (PAHs). Esta nebulosa, por estar exposta a intensos campos de UV e Raios-X, é uma região de fotodissociação molecular que propicia a formação de novas moléculas, confirmada pela presença de C4H2 e C6H6 (Benzeno), que é a unidade básica dos PAHs. Atribui-se a esta família de moléculas orgânicas duas propriedades fundamentais, a resistência para sobreviver ao campo de radiação UV interestelar e a geração das bandas de emissão não identificadas (UIR) observadas no infravermelho. No entanto, alguns autores questionam a resistência dos PAHs ao campo de radiação UV interestelar. Empregando a técnica de Espectrometria de Massas por Tempo de Vôo, no modo de coincidência fotoelétron-fotoíon, estudamos a ionização e fragmentação das seguintes moléculas: Benzeno, Benzeno deuterado, Naftaleno, Antraceno e Fenantreno. Utilizamos uma fonte de Hélio monocromática em 21,21 eV (584,5 Å) e a radiação Síncroton do Laboratório Nacional de Luz Síncroton (LNLS) em diferentes energias nas proximidades da borda do C 1s ( 290 eV). Comprovamos a estabilidade dos PAHs sob ação de UV (21,21 eV), onde eles apresentam um baixo nível de fotodissociação, produzindo fragmentos ionizados com rendimento total na ordem de 5 por cento em relação ao íon molecular pai. Entretanto, em altas energias, na faixa de Raios-X, a quebra destas moléculas torna-se mais intensa, com a produção de muitos fragmentos. Como uma das rotas de fragmentação do Naftaleno é [(C10H8) = > (C6H6+) + (C4H2) + (e-)], e como temos as evidências observacionais da existência do C4H2 e C6H6 na nebulosa CRL 618, sugerimos que este ambiente também possui o Naftaleno.

  7. Divergent regulation by growth factors and cytokines of 95 kDa and 72 kDa gelatinases and tissue inhibitors or metalloproteinases-1, -2, and -3 in rabbit aortic smooth muscle cells.

    PubMed

    Fabunmi, R P; Baker, A H; Murray, E J; Booth, R F; Newby, A C

    1996-04-01

    The migration and proliferation of vascular smooth muscle cells (SMCs) during neointima formation in atherosclerosis and angioplasty restenosis is mediated by certain growth factors and cytokines, one action of which may be to promote basement-membrane degradation. To test this hypothesis further, the effects of such growth factors and cytokines on the synthesis of two basement-membrane-degrading metalloproteinases, namely the 72 kDa gelatinase (MMP-2, gelatinase A) and the 95 kDa gelatinase (MMP-9, gelatinase B) and three tissue inhibitors of metalloproteinases (TIMPs) was studied in primary cultured rabbit aortic SMCs. Expression of the 95 kDa gelatinase was increased by phorbol myristate acetate, foetal calf serum, thrombin and interleukin-1alpha (IL-1alpha); platelet-derived growth factor (PDGF) BB alone had no effect but acted synergistically with IL-1alpha. A selective protein kinase C inhibitor, Ro 31-8220, abolished induction of the 95 kDa gelatinase. In contrast, none of the agents tested modulated the synthesis of the 72 kDa gelatinase. We conclude that maximal up-regulation of 95 kDa gelatinase expression requires the concerted action of growth factors and inflammatory cytokines mediated, in part, by a protein kinase C-dependent pathway. TIMP-1 and TIMP-2 were highly expressed, and their synthesis was not affected by growth factors or cytokines. Expression of TIMP-3 mRNAs was, however, increased by PDGF and transforming growth factor beta, especially in combination. Divergent regulation of gelatinase and TIMP expression implies that either net synthesis or net degradation of basement membrane can be mediated by appropriate combinations of growth factors and cytokines. PMID:8670128

  8. Divergent regulation by growth factors and cytokines of 95 kDa and 72 kDa gelatinases and tissue inhibitors or metalloproteinases-1, -2, and -3 in rabbit aortic smooth muscle cells.

    PubMed Central

    Fabunmi, R P; Baker, A H; Murray, E J; Booth, R F; Newby, A C

    1996-01-01

    The migration and proliferation of vascular smooth muscle cells (SMCs) during neointima formation in atherosclerosis and angioplasty restenosis is mediated by certain growth factors and cytokines, one action of which may be to promote basement-membrane degradation. To test this hypothesis further, the effects of such growth factors and cytokines on the synthesis of two basement-membrane-degrading metalloproteinases, namely the 72 kDa gelatinase (MMP-2, gelatinase A) and the 95 kDa gelatinase (MMP-9, gelatinase B) and three tissue inhibitors of metalloproteinases (TIMPs) was studied in primary cultured rabbit aortic SMCs. Expression of the 95 kDa gelatinase was increased by phorbol myristate acetate, foetal calf serum, thrombin and interleukin-1alpha (IL-1alpha); platelet-derived growth factor (PDGF) BB alone had no effect but acted synergistically with IL-1alpha. A selective protein kinase C inhibitor, Ro 31-8220, abolished induction of the 95 kDa gelatinase. In contrast, none of the agents tested modulated the synthesis of the 72 kDa gelatinase. We conclude that maximal up-regulation of 95 kDa gelatinase expression requires the concerted action of growth factors and inflammatory cytokines mediated, in part, by a protein kinase C-dependent pathway. TIMP-1 and TIMP-2 were highly expressed, and their synthesis was not affected by growth factors or cytokines. Expression of TIMP-3 mRNAs was, however, increased by PDGF and transforming growth factor beta, especially in combination. Divergent regulation of gelatinase and TIMP expression implies that either net synthesis or net degradation of basement membrane can be mediated by appropriate combinations of growth factors and cytokines. PMID:8670128

  9. Genes influencing spinal bone mineral density in inbred F344, LEW, COP, and DA rats

    PubMed Central

    Alam, Imranul; Sun, Qiwei; Koller, Daniel L.; Liu, Lixiang; Liu, Yunlong; Edenberg, Howard J.; Foroud, Tatiana

    2009-01-01

    Previously, we identified the regions of chromosomes 10q12–q31 and 15p16–q21 harbor quantitative trait loci (QTLs) for lumbar volumetric bone mineral density (vBMD) in female F2 rats derived from Fischer 344 (F344) × Lewis (LEW) and Copenhagen 2331 (COP) × Dark Agouti (DA) crosses. The purpose of this study is to identify the candidate genes within these QTL regions contributing to the variation in lumbar vBMD. RNA was extracted from bone tissue of F344, LEW, COP, and DA rats. Microarray analysis was performed using Affymetrix Rat Genome 230 2.0 Arrays. Genes differentially expressed among the rat strains were then ranked based on the strength of the correlation with lumbar vBMD in F2 animals derived from these rats. Quantitative PCR (qPCR) analysis was performed to confirm the prioritized candidate genes. A total of 285 genes were differentially expressed among all strains of rats with a false discovery rate less than 10%. Among these genes, 18 candidate genes were prioritized based on their strong correlation (r2 > 0.90) with lumbar vBMD. Of these, 14 genes (Akap1, Asgr2, Esd, Fam101b, Irf1, Lcp1, Ltc4s, Mdp-1, Pdhb, Plxdc1, Rabep1, Rhot1, Slc2a4, Xpo4) were confirmed by qPCR. We identified several novel candidate genes influencing spinal vBMD in rats. PMID:19841953

  10. Studies in pig heart tissue on various 60,000 Da phosphoproteins.

    PubMed

    Guesdon, F; David-Pfeuty, T

    1989-03-01

    Pig heart tissue have been shown to contain 3 different 60,000 Da phosphoproteins. Different purification procedures were used in order to separate them, suggesting that the 3 phosphoproteins differ in their environmental parameters. The 2 major ones appear essentially as peripheral phosphoproteins that are associated with cellular membranes through ionic forces, whereas the third minor phosphoprotein behaves as an integral plasma membrane protein. The three phosphoproteins also differ in their relative amount of phosphorylated serine, threonine and tyrosine residues after in vitro protein kinase assay. Evidence that the 3 phosphoproteins are related arises from the similarity between their respective phosphopeptide maps after partial hydrolysis with proteases, an experiment that also points out relatedness in primary structure between them and the transforming protein of Rous sarcoma virus, pp60v-src. The 3 phosphoproteins, however, do not appear to be immunologically related to pp60v-src since none of them is immunoprecipitated by sera that precipitate pp60v-src. The possibility that the three 60,000 Da phosphoproteins under study represent 3 differentially localized and phosphorylated products of c-src and/or c-src related genes is discussed. PMID:2472841

  11. 3D-printing of transparent bio-microfluidic devices in PEG-DA.

    PubMed

    Urrios, Arturo; Parra-Cabrera, Cesar; Bhattacharjee, Nirveek; Gonzalez-Suarez, Alan M; Rigat-Brugarolas, Luis G; Nallapatti, Umashree; Samitier, Josep; DeForest, Cole A; Posas, Francesc; Garcia-Cordero, José L; Folch, Albert

    2016-06-21

    The vast majority of microfluidic systems are molded in poly(dimethylsiloxane) (PDMS) by soft lithography due to the favorable properties of PDMS: biocompatible, elastomeric, transparent, gas-permeable, inexpensive, and copyright-free. However, PDMS molding involves tedious manual labor, which makes PDMS devices prone to assembly failures and difficult to disseminate to research and clinical settings. Furthermore, the fabrication procedures limit the 3D complexity of the devices to layered designs. Stereolithography (SL), a form of 3D-printing, has recently attracted attention as a way to customize the fabrication of biomedical devices due to its automated, assembly-free 3D fabrication, rapidly decreasing costs, and fast-improving resolution and throughput. However, existing SL resins are not biocompatible and patterning transparent resins at high resolution remains difficult. Here we report procedures for the preparation and patterning of a transparent resin based on low-MW poly(ethylene glycol) diacrylate (MW 250) (PEG-DA-250). The 3D-printed devices are highly transparent and cells can be cultured on PEG-DA-250 prints for several days. This biocompatible SL resin and printing process solves some of the main drawbacks of 3D-printed microfluidic devices: biocompatibility and transparency. In addition, it should also enable the production of non-microfluidic biomedical devices. PMID:27217203

  12. Integration of Globus Online with the ATLAS PanDA Workload Management System

    NASA Astrophysics Data System (ADS)

    Contreras, C.; Deng, W.; Maeno, T.; Nilsson, P.; Potekhin, M.

    2012-12-01

    The PanDA Workload Management System is the basis for distributed production and analysis for the ATLAS experiment at the LHC. In this role, it relies on sophisticated dynamic data movement facilities developed in ATLAS. In certain scenarios, such as small research teams in ATLAS Tier-3 sites and non-ATLAS Virtual Organizations, the overhead of installation and operation of these components makes their use not very cost effective. Globus Online is an emerging new tool from the Globus Alliance, which already proved popular within the research community. It provides the users with fast and robust file transfer capabilities that can also be managed from a Web interface, and in addition to grid sites, can have individual workstations and laptops serving as data transmission endpoints. We will describe the integration of the Globus Online functionality into the PanDA suite of software, in order to give more flexibility in choosing the method of data transfer to ATLAS Tier-3 and Open Science Grid (OSG) users.

  13. VaccineDA: Prediction, design and genome-wide screening of oligodeoxynucleotide-based vaccine adjuvants

    PubMed Central

    Nagpal, Gandharva; Gupta, Sudheer; Chaudhary, Kumardeep; Kumar Dhanda, Sandeep; Prakash, Satya; Raghava, Gajendra P. S.

    2015-01-01

    Immunomodulatory oligodeoxynucleotides (IMODNs) are the short DNA sequences that activate the innate immune system via toll-like receptor 9. These sequences predominantly contain unmethylated CpG motifs. In this work, we describe VaccineDA (Vaccine DNA adjuvants), a web-based resource developed to design IMODN-based vaccine adjuvants. We collected and analyzed 2193 experimentally validated IMODNs obtained from the literature. Certain types of nucleotides (e.g., T, GT, TC, TT, CGT, TCG, TTT) are dominant in IMODNs. Based on these observations, we developed support vector machine-based models to predict IMODNs using various compositions. The developed models achieved the maximum Matthews Correlation Coefficient (MCC) of 0.75 with an accuracy of 87.57% using the pentanucleotide composition. The integration of motif information further improved the performance of our model from the MCC of 0.75 to 0.77. Similarly, models were developed to predict palindromic IMODNs and attained a maximum MCC of 0.84 with the accuracy of 91.94%. These models were evaluated using a five-fold cross-validation technique as well as validated on an independent dataset. The models developed in this study were integrated into VaccineDA to provide a wide range of services that facilitate the design of DNA-based vaccine adjuvants (http://crdd.osdd.net/raghava/vaccineda/). PMID:26212482

  14. Crystal Structure of the 25 kDa Subunit of Human Cleavage Factor I{m}

    SciTech Connect

    Coseno,M.; Martin, G.; Berger, C.; Gilmartin, G.; Keller, W.; Doublie, S.

    2008-01-01

    Cleavage factor Im is an essential component of the pre-messenger RNA 3'-end processing machinery in higher eukaryotes, participating in both the polyadenylation and cleavage steps. Cleavage factor Im is an oligomer composed of a small 25 kDa subunit (CF Im25) and a variable larger subunit of either 59, 68 or 72 kDa. The small subunit also interacts with RNA, poly(A) polymerase, and the nuclear poly(A)-binding protein. These protein-protein interactions are thought to be facilitated by the Nudix domain of CF Im25, a hydrolase motif with a characteristic {alpha}/{beta}/{alpha} fold and a conserved catalytic sequence or Nudix box. We present here the crystal structures of human CF Im25 in its free and diadenosine tetraphosphate (Ap4A) bound forms at 1.85 and 1.80 Angstroms, respectively. CF Im25 crystallizes as a dimer and presents the classical Nudix fold. Results from crystallographic and biochemical experiments suggest that CF Im25 makes use of its Nudix fold to bind but not hydrolyze ATP and Ap4A. The complex and apo protein structures provide insight into the active oligomeric state of CF Im and suggest a possible role of nucleotide binding in either the polyadenylation and/or cleavage steps of pre-messenger RNA 3'-end processing.

  15. Leonardo da Vinci and Andreas Vesalius; the shoulder girdle and the spine, a comparison.

    PubMed

    Ganseman, Y; Broos, P

    2008-01-01

    Leonardo Da Vinci and Andreas Vesalius were two important renaissance persons; Vesalius was a surgeon-anatomist who delivered innovative work on the study of the human body, Leonardo da Vinci was an artist who delivered strikingly accurate and beautiful drawings on the human body. Below we compare both masters with regard to their knowledge of the working of the muscles, their method and system of dissection and their system and presentation of the drawings. The investigation consisted of a comparison between both anatomists, in particular concerning their study on the shoulder girdle and spine, by reviewing their original work as well as already existing literature on this subject. The investigation led to the conclusion that the drawings mentioned meant a change in history, and were of high quality, centuries ahead of their time. Both were anatomists, both were revolutionary, only one changed history at the moment itself, while the other changed history centuries later. Leonardo has made beautiful drawings that are at a match with the drawings of today or are even better. Vesalius set the start for medicine as a science as it is until this day. Their lives differed as strongly as their impact. In the light of their time, the achievement they made was extraordinary. PMID:18807610

  16. UniDA: Uniform Device Access Framework for Human Interaction Environments

    PubMed Central

    Varela, Gervasio; Paz-Lopez, Alejandro; Becerra, Jose Antonio; Vazquez-Rodriguez, Santiago; Duro, Richard José

    2011-01-01

    Human interaction environments (HIE) must be understood as any place where people carry out their daily life, including their work, family life, leisure and social life, interacting with technology to enhance or facilitate the experience. The integration of technology in these environments has been achieved in a disorderly and incompatible way, with devices operating in isolated islands with artificial edges delimited by the manufacturers. In this paper we are presenting the UniDA framework, an integral solution for the development of systems that require the integration and interoperation of devices and technologies in HIEs. It provides developers and installers with a uniform conceptual framework capable of modelling an HIE, together with a set of libraries, tools and devices to build distributed instrumentation networks with support for transparent integration of other technologies. A series of use case examples and a comparison to many of the existing technologies in the field has been included in order to show the benefits of using UniDA. PMID:22163700

  17. Blood species identification using Near-Infrared diffuse transmitted spectra and PLS-DA method

    NASA Astrophysics Data System (ADS)

    Zhang, Linna; Zhang, Shengzhao; Sun, Meixiu; Wang, Zhennan; Li, Hongxiao; Li, Yingxin; Li, Gang; Lin, Ling

    2016-05-01

    Blood species identification is of great significance for blood supervision and wildlife investigations. The current methods used to identify the blood species are destructive. Near-Infrared spectroscopy method is known for its non-invasive properties. In this research, we combined Near-Infrared diffuse transmitted spectra and Partial Least Square Discrimination Analysis (PLS-DA) to identify three blood species, including macaque, human and mouse. Blind test and external test were used to assess the PLS-DA model. The model performed 100% accuracy in its identification between three blood species. This approach does not require a specific knowledge of biochemical features for each individual class but relies on a spectroscopic statistical differentiation of the whole components. This is the first time to demonstrate Near-Infrared diffuse transmitted spectra have the ability to identify the species of origin of blood samples. The results also support a good potential of absorption and scattering spectroscopy for species identification in practical applications for real-time detection.

  18. Interactions of the 67 kDa laminin receptor and its precursor with laminin

    PubMed Central

    Fatehullah, Aliya; Doherty, Caroline; Pivato, Géraldine; Allen, George; Devine, Lynda; Nelson, John; Timson, David J.

    2009-01-01

    The 67LR (67 kDa laminin receptor) enables cells to interact with components of the extracellular matrix. The molecule is derived from the 37LRP (37 kDa laminin receptor precursor); however, the precise molecular mechanism of this conversion is unknown. Recombinant 37LRP, expressed in and purified from Escherichia coli, bound to human laminin in a SPR (surface plasmon resonance) experiment. 67LR isolated from human breast-cancer-derived cells in culture was also shown to bind to laminin by SPR. However, the kinetics of association are qualitatively different. 37LRP, but not 67LR, binds to heparan sulfate. The binding of 37LRP to heparan sulfate did not affect the interaction of 37LRP with laminin. In contrast, heparan sulfate reduces the extent of binding of laminin to 67LR. Taken together, these results show that 37LRP has some of the biological activities of 67LR, even prior to the conversion event. However, the conversion affects the sites of interaction with both laminin and heparan sulfate. PMID:19691449

  19. The uncatchable smile in Leonardo da Vinci's La Bella Principessa portrait.

    PubMed

    Soranzo, Alessandro; Newberry, Michelle

    2015-08-01

    A portrait of uncertain origin recently came to light which, after extensive research and examination, was shown to be that rarest of things: a newly discovered Leonardo da Vinci painting entitled La Bella Principessa. This research presents a new illusion which is similar to that identified in the Mona Lisa; La Bella Principessa's mouth appears to change slant depending on both the Viewing Distance and the Level of Blur applied to a digital version of the portrait. Through a series of psychophysics experiments, it was found that a perceived change in the slant of the La Bella Principessa's mouth influences her expression of contentment thus generating an illusion that we have coined the "uncatchable smile". The elusive quality of the Mona Lisa's smile has been previously reported (Science, 290 (2000) 1299) and so the existence of a similar illusion in a portrait painted prior to the Mona Lisa becomes more interesting. The question remains whether Leonardo da Vinci intended this illusion. In any case, it can be argued that the ambiguity created adds to the portrait's allure. PMID:26049039

  20. Gentile da Foligno, a pioneer of cardionephrology: commentary on Carmina de urinarum iudiciis and De pulsibus.

    PubMed

    Timio, M

    1999-01-01

    Gentile da Foligno was an influential professor and teacher of medicine at the Universities of Bologna, Padua, Siena and Perugia in the first part of the 14th century. He was one of the first physicians to perform human dissection. He authored many important texts and commentaries, among which is the Consilla, a collection of therapeutic advices for many diseases. He made a commentary on Carmina de urinarum iudiciis (Songs of urinary judgements) and to De pulsibus (About pulses) composed by Egidius Corbaliensis. This work is more than a commentary: it is a text attempting to conceptualise the physiology of urine formation. According to his writing, urine associated to the blood passes 'per poros euritides' (through the porous tubules) of the kidney and is then delivered to the bladder. Commenting and explaining the writing on De pulsibus, he stressed the importance of heart disease on modulating the colour and output of urine and the relationship between fast pulse rate and urine output. For the originality of his thought and for the conceptualization of the relationship between pulse rate and urine characteristic, Gentile da Foligno could be indicated as the 'first' cardionephrologist in the history of medicine. PMID:10213818

  1. Magnetisches Tracking für die Navigation mit dem da Vinci® Surgical System

    NASA Astrophysics Data System (ADS)

    Nickel, Felix; Wegner, Ingmar; Kenngott, Hannes; Neuhaus, Jochen; Müller-Stich, Beat P.; Meinzer, Hans-Peter; Gutt, Carsten N.

    In dieser Studie wurde untersucht ob in einem typischen OP-Aufbau mit dem da Vinci® Telemanipulator elektromagnetisches Tracking für die Realisation eines Navigationssystems eingesetzt werden kann. Hierfür wurde in einem realen OP-Aufbau untersucht, wie stark metallische und ferromagnetisch wirksame Objekte wie Operationstisch und Telemanipulator das elektromagnetische Feld des Trackingsystems beeinflussen. Die Ergebnisse zeigen, dass der Telemanipulator nur unwesentlich die Störung des Magnetfeldes durch den OP-Tisch verstärkt. Insbesondere die Bewegung der Instrumente im Trackingvolumen verursachte keine zusätzliche relevante Störung des Magnetfeldes. Bei Begrenzung des Trackingvolumens auf eine Länge von 190 mm, Höhe von 200mm und Breite von 400 mm war der maximale Fehler in diesem Bereich an allen Messpunkten kleiner 10 mm. Der Einsatz von elektromagnetischem Tracking für die Navigation mit dem da Vinci® Surgical System ist somit in einem begrenzten Arbeitsvolumen mit hinreichender Genauigkeit möglich.

  2. Time Resolved Detection of Infrared Synchrotron Radiation at DA{phi}NE

    SciTech Connect

    Bocci, A.; Marcelli, A.; Drago, A.; Guidi, M. Cestelli; Pace, E.; Piccinini, M.; Sali, D.; Morini, P.

    2007-01-19

    Synchrotron radiation is characterized by a very wide spectral emission from IR to X-ray wavelengths and a pulsed structure that is a function of the source time structure. In a storage ring, the typical temporal distance between two bunches, whose duration is a few hundreds of picoseconds, is on the nanosecond scale. Therefore, synchrotron radiation sources are a very powerful tools to perform time-resolved experiments that however need extremely fast detectors. Uncooled IR devices optimized for the mid-IR range with sub-nanosecond response time, are now available and can be used for fast detection of intense IR sources such as synchrotron radiation storage rings. We present here different measurements of the pulsed synchrotron radiation emission at DA{phi}NE (Double Annular {phi}-factory for Nice Experiments), the collider of the Laboratori Nazionali of Frascati (LNF) of the Istituto Nazionale di Fisica Nucleare (INFN), performed with very fast uncooled infrared detectors with a time resolution of a few hundreds of picoseconds. We resolved the emission time structure of the electron bunches of the DA{phi}NE collider when it works in a normal condition for high energy physics experiments with both photovoltaic and photoconductive detectors. Such a technology should pave the way to new diagnostic methods in storage rings, monitoring also source instabilities and bunch dynamics.

  3. [History of robotics: from Archytas of Tarentum until da Vinci robot. (Part I)].

    PubMed

    Sánchez Martín, F M; Millán Rodríguez, F; Salvador Bayarri, J; Palou Redorta, J; Rodríguez Escovar, F; Esquena Fernández, S; Villavicencio Mavrich, H

    2007-02-01

    Robotic surgery is the newst technologic option in urology. To understand how new robots work is interesting to know their history. The desire to design machines imitating humans continued for more than 4000 years. There are references to King-su Tse (clasic China) making up automaton at 500 a. C. Archytas of Tarentum (at around 400 a.C.) is considered the father of mechanical engineering, and one of the occidental robotics classic referents. Heron of Alexandria, Hsieh-Fec, Al-Jazari, Roger Bacon, Juanelo Turriano, Leonardo da Vinci, Vaucanson o von Kempelen were robot inventors in the middle age, renaissance and classicism. At the XIXth century, automaton production underwent a peak and all engineering branches suffered a great development. At 1942 Asimov published the three robotics laws, based on mechanics, electronics and informatics advances. At XXth century robots able to do very complex self governing works were developed, like da Vinci Surgical System (Intuitive Surgical Inc, Sunnyvale, CA, USA), a very sophisticated robot to assist surgeons. PMID:17645084

  4. Development of noSQL data storage for the ATLAS PanDA Monitoring System

    NASA Astrophysics Data System (ADS)

    Ito, H.; Potekhin, M.; Wenaus, T.

    2012-12-01

    For several years the PanDA Workload Management System has been the basis for distributed production and analysis for the ATLAS experiment at the LHC. Since the start of data taking PanDA usage has ramped up steadily, typically exceeding 500k completed jobs/day by June 2011. The associated monitoring data volume has been rising as well, to levels that present a new set of challenges in the areas of database scalability and monitoring system performance and efficiency. These challenges are being met with an R&D effort aimed at implementing a scalable and efficient monitoring data storage based on a noSQL solution (Cassandra). We present our motivations for using this technology, as well as data design and the techniques used for efficient indexing of the data. We also discuss the hardware requirements as they were determined by testing with actual data and realistic rate of queries. In conclusion, we present our experience with operating a Cassandra cluster over an extended period of time and with data load adequate for planned application.

  5. Food residue recycling by swine breeders in a developing economy: a case study in Da Nang, Viet Nam.

    PubMed

    Kato, Takaaki; Pham, Dung Thi Xuan; Hoang, Hai; Xue, Yonghai; Tran, Quang Van

    2012-12-01

    This study provides a detailed description of food residue collection by swine breeders in Da Nang, Viet Nam. In January 2011, the study surveyed 30 swine breeders in two villages with respect to locations, methods, prices, quantities, and prospects for food residue collection. The sampled swine breeders regularly visited 55 locations in central Da Nang to collect raw food residue. They then transferred the food residue to their piggeries, boiled it, and fed it to their swine. A regression analysis revealed that the total amount of food residue collected by a farm depends on the number of swine in the farm and the number of collections made per day. Swine breeders in Da Nang were estimated to collect 26.3 metric tons of organic waste per day, which amounted to 4.1% of domestic waste collected by the local government. Among the sampled swine breeders, 93% answered that they would continue using food residue for the next five years. PMID:22883688

  6. Análise Temporal da Coma de CO+ no Cometa P/Halley

    NASA Astrophysics Data System (ADS)

    Voelzke, M. R.; Schlosser, W.; Schmidt-Kaler, Th.

    1995-08-01

    Observações fotográficas e fotoelétricas da coma de gás ionizado do cometa P/Halley a nível de CO+ em 4250 angstroms fizeram parte do programa de Monitoramento do Halley desenvolvido pela Universidade de Bochum (Ruhr-Universitaet Bochum) na Alemanha, realizado de 17 de fevereiro a 17 de abril de 1986 no European Southern Observatory (ESO) em La Silla (Chile). Nesta faixa espectral é possível observar a contínua formação, bem como o movimento e expansão das estruturas de plasma. Para observar a morfologia destas estruturas foram analisadas 32 placas fotográficas de CO+ (placas de vidro) do cometa P/Halley. Tais placas possuem um campo de visão de 28,6 por 28,6 graus sendo obtidas entre 29 de março e 17 de abril de 1986 com tempos de exposição entre 20 e 120 minutos. Todas as placas foram digitadas com o auxílio de um microdensitômetro PDS 2020 GM (Photometric Data System) do Instituto Astronómico da Westfaelischen Wilhelms-Universitaet em Muenster, Alemanha (um pixel = 25 por 25 micrômetros correspondendo aproximadamente a 46,88 por 46,88 segundos de arco). Após a digitação os dados foram reduzidos à intensidades relativas, sendo que os posssíveis de calibração também foram reduzidos à intensidades absolutas, expressas em termos de densidade colunar utilizando-se dos sistemas de tratamento de imagens MIDAS (Munich Image Data Analysis System; ESO - Image Processing Group, 1988) e IHAP (Image Handling And Processing; Middleburg, 1983). Com o auxílio do método de teta mínimo de Stellingwerf (Stellingwerf, 1978) obteve-se um período de 2,22 +/- 0,09 dias a partir da análise de estruturas na coma de plasma através da subtração de imagens subsequentes. Este método foi comparado com o método de Fourier. Provavelmente exista um segundo ciclo com período aproximado de 3,6 dias. A idéia de subtrair imagens subsequentes é devido ao fato de que os efeitos de rotação são apenas 10% dos fenômenos de distribuição gasosa. Portanto as

  7. Expression, purification, and characterization of a bifunctional 99-kDa peptidoglycan hydrolase from Pediococcus acidilactici ATCC 8042.

    PubMed

    García-Cano, Israel; Campos-Gómez, Manuel; Contreras-Cruz, Mariana; Serrano-Maldonado, Carlos Eduardo; González-Canto, Augusto; Peña-Montes, Carolina; Rodríguez-Sanoja, Romina; Sánchez, Sergio; Farrés, Amelia

    2015-10-01

    Pediococcus acidilactici ATCC 8042 is a lactic acid bacteria that inhibits pathogenic microorganisms such as Staphylococcus aureus through the production of two proteins with lytic activity, one of 110 kDa and the other of 99 kDa. The 99-kDa one has high homology to a putative peptidoglycan hydrolase (PGH) enzyme reported in the genome of P. acidilactici 7_4, where two different lytic domains have been identified but not characterized. The aim of this work was the biochemical characterization of the recombinant enzyme of 99 kDa. The enzyme was cloned and expressed successfully and retains its activity against Micrococcus lysodeikticus. It has a higher N-acetylglucosaminidase activity, but the N-acetylmuramoyl-L-alanine amidase can also be detected spectrophotometrically. The protein was then purified using gel filtration chromatography. Antibacterial activity showed an optimal pH of 6.0 and was stable between 5.0 and 7.0. The optimal temperature for activity was 60 °C, and all activity was lost after 1 h of incubation at 70 °C. The number of strains susceptible to the recombinant 99-kDa enzyme was lower than that susceptible to the mixture of the 110- and 99-kDa PGHs of P. acidilactici, a result that suggests synergy between these two enzymes. This is the first PGH from LAB that has been shown to possess two lytic sites. The results of this study will aid in the design of new antibacterial agents from natural origin that can combat foodborne disease and improve hygienic practices in the industrial sector. PMID:25940238

  8. Culture and Dehydration: A Comparative Study of Caída de la Mollera (Fallen Fontanel) in Three Latino Populations.

    PubMed

    Pachter, Lee M; Weller, Susan C; Baer, Roberta D; de Alba Garcia, Javier E Garcia; Glazer, Mark; Trotter, Robert; Klein, Robert E; Gonzalez, Eduardo

    2016-10-01

    A sunken soft-spot or fontanel is a sign for dehydration in infants. Around the world, folk illnesses, such as caída de la mollera in some Latin American cultures, often incorporate this sign as a hallmark of illness, but may or may not incorporate re-hydration therapies in treatment strategies. This report describes a study of lay descriptions of causes, symptoms, and treatments for caída de la mollera in three diverse Latin American populations. A mixed-methods approach was used. Representative community-based samples were interviewed in rural Guatemala, Guadalajara, Mexico, and Edinburgh, Texas, with a 132 item questionnaire on the causes, susceptibility, symptoms, and therapies for caída de la mollera. Cultural consensus analysis was used to estimate community beliefs about caída. Interviews conducted in rural Guatemala (n = 60), urban Mexico (n = 62), and rural Texas on the Mexican border (n = 61) indicated consistency in thematic elements within and among these three diverse communities. The high degree of consistency in the illness explanatory models indicated shared beliefs about caída de la mollera in each of the communities and a core model shared across communities. However, an important aspect of the community beliefs was that rehydration therapies were not widely endorsed. The consistency in explanatory models in such diverse communities, as well as the high degree of recognition and experience with this illness, may facilitate communication between community members, and health care providers/public health intervention planners to increase use of rehydration therapies for caída de la mollera. Recommendations for culturally informed and respectful approaches to clinical communication are provided. PMID:26239172

  9. Membrane-associated 41-kDa GTP-binding protein in collagen-induced platelet activation

    SciTech Connect

    Walker, G.; Bourguignon, L.Y. )

    1990-08-01

    Initially we established that the binding of collagen to human blood platelets stimulates both the rapid loss of PIP2 and the generation of inositol-4,5-bisphosphate (IP2) and inositol-1,4,5-triphosphate (IP3). These results indicate that the binding of collagen stimulates inositol phospholipid-specific phospholipase C during platelet activation. The fact that GTP or GTP-gamma-S augments, and pertussis toxin inhibits, collagen-induced IP3 formation suggests that a GTP-binding protein or (or proteins) may be directly involved in the regulation of phospholipase C-mediated phosphoinositide turnover in human platelets. We have used several complementary techniques to isolate and characterize a platelet 41-kDa polypeptide (or polypeptides) that has a number of structural and functional similarities to the regulatory alpha i subunit of the GTP-binding proteins isolated from bovine brain. This 41-kDa polypeptide (or polypeptides) is found to be closely associated with at least four membrane glycoproteins (e.g., gp180, gp110, gp95, and gp75) in a 330-kDa complex that can be dissociated by treatment with high salt plus urea. Most important, we have demonstrated that antilymphoma 41-kDa (alpha i subunit of GTP-binding proteins) antibody cross-reacts with the platelet 41-kDa protein (or proteins) and the alpha i subunit of bovine brain Gi alpha proteins, and blocks GTP/collagen-induced IP3 formation. These data provide strong evidence that the 41-kDa platelet GTP-binding protein (or proteins) is directly involved in collagen-induced signal transduction during platelet activation.

  10. Purification and characteristics of a 30 kDa Cd-binding protein from rat testis cytosol

    SciTech Connect

    Kinoshita, C.M.

    1986-01-01

    The main objective of this dissertation was to purify the 30 kDa Cd-binding protein from rat testis cytosol (Cd-testin) in a form retaining 1 or more moles of Cd per mole of protein, following the addition of /sup 109/CdCl/sub 2/ to cytosol in amounts just sufficient to saturate the 30 kDa peak. Two successful procedures were developed based on (1) ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration; or (2) heat treatment (90/sup 0/C, 5 min) and gel filtration. Application of Method 1 to cytosol labeled with /sup 65/ZnCL/sub 2/ showed that Zn formed a complex analogous to Cd-testin. The Cd content (moles/mole protein) in two preparations of 30 kDa protein was 2.1 to 2.6 (Method 1); Method 2 gave more variable results (1.1 to 3.4). In neither case was a 30 kDa polypeptide detected by 20 SDS PAGE, but a major subunit (22 kDa/pl 4.6) was observed in both preparations, along with smaller subunits. Method 1 gave higher purity (3 spots) but lower yield (about 4% of cytosolic 30 kDa Cd) whereas Method 2 gave a much higher yield (about 45%) but somewhat lower purity based on 2D SDS PAGE. On the basis of these results, Cd-testin consists of subunits rather than a single polypeptide chain. It is concluded that rat testis contains novel proteins binding Cd or Zn, and can be isolated in forms that retain a high Cd content. The manner in which the Cd is bound, and the subunits involved, have not been determined.

  11. gLExec and MyProxy integration in the ATLAS/OSG PanDA workload management system

    NASA Astrophysics Data System (ADS)

    Caballero, J.; Hover, J.; Litmaath, M.; Maeno, T.; Nilsson, P.; Potekhin, M.; Wenaus, T.; Zhao, X.

    2010-04-01

    Worker nodes on the grid exhibit great diversity, making it difficult to offer uniform processing resources. A pilot job architecture, which probes the environment on the remote worker node before pulling down a payload job, can help. Pilot jobs become smart wrappers, preparing an appropriate environment for job execution and providing logging and monitoring capabilities. PanDA (Production and Distributed Analysis), an ATLAS and OSG workload management system, follows this design. However, in the simplest (and most efficient) pilot submission approach of identical pilots carrying the same identifying grid proxy, end-user accounting by the site can only be done with application-level information (PanDA maintains its own end-user accounting), and end-user jobs run with the identity and privileges of the proxy carried by the pilots, which may be seen as a security risk. To address these issues, we have enabled PanDA to use gLExec, a tool provided by EGEE which runs payload jobs under an end-user's identity. End-user proxies are pre-staged in a credential caching service, MyProxy, and the information needed by the pilots to access them is stored in the PanDA DB. gLExec then extracts from the user's proxy the proper identity under which to run. We describe the deployment, installation, and configuration of gLExec, and how PanDA components have been augmented to use it. We describe how difficulties were overcome, and how security risks have been mitigated. Results are presented from OSG and EGEE Grid environments performing ATLAS analysis using PanDA and gLExec.

  12. Ontogeny of DA1 receptor-mediated natriuresis in the rat: in vivo and in vitro correlations.

    PubMed

    Kaneko, S; Albrecht, F; Asico, L D; Eisner, G M; Robillard, J E; Jose, P A

    1992-09-01

    The natriuretic and diuretic effects of dopamine are attenuated in the young. Because dopamine has actions on receptors (e.g., adrenergic, serotonin) other than dopamine, we studied a novel dopamine agonist, pramipexole, which has a selectivity to both DA1 and DA2-receptor subtypes. Intravenous administration of pramipexole resulted in a dose-related (1, 10, and 100 micrograms.kg-1.min-1) increase in urine flow and absolute and fractional sodium excretion and a decrease in mean arterial pressure (MAP) in three groups of rats studied. Pramipexole induced a greater decrease in MAP in 6- to 7- (n = 5) and 9- to 16- (n = 6) than in 3- to 4-wk-old (n = 8) rats; the natriuresis and diuresis were greatest in 12- to 16- and least in 3- to 4-wk-old rats. The renal effects of pramipexole were mainly due to actions at the DA1 receptor, since these effects were completely blocked by the coinfusion of a DA1 antagonist, SKF 83742. To explore further a cause of the attenuated natriuretic effect of pramipexole in the young, we studied the effect of a selective DA1-receptor agonist, fenoldopam, on amiloride-sensitive 22Na+ uptake in renal brush-border membrane vesicles. The 3-s amiloride-sensitive uptake was inhibited (45%) by fenoldopam (5 x 10(-5)M) in 9- to 16- (n = 6) but not in 3- to 4-wk-old (n = 5) rats. These studies suggest that the attenuated natriuretic effect of dopamine in the young is in part due to decreased DA1 action on the brush-border membrane Na(+)-H+ exchanger. PMID:1357983

  13. Hopf-Ranãda linked and knotted light beam solution viewed as a null electromagnetic field.

    PubMed

    Besieris, Ioannis M; Shaarawi, Amr M

    2009-12-15

    The Hopf-Ranãda linked and knotted light beam solution, which has been interpreted physically and extended analytically by Irvine and Bouwmeester recently, is viewed in this Letter as a null electromagnetic field. It is shown, in particular, that the Hopf-Ranãda solution is a variant of a luminal null electromagnetic wave due originally to Robinson and Troutman and reported by Bialynicki-Birula recently. This analogy is motivated by means of a method due to Whittaker and Bateman, and a relationship to well-known scalar luminal localized waves is examined. PMID:20016647

  14. Genome Sequence of the Psychrophilic Bacterium Tenacibaculum ovolyticum Strain da5A-8 Isolated from Deep Seawater

    PubMed Central

    Zhai, Zhenyu; Komatsu, Ayumi; Shibayama, Keigo

    2016-01-01

    Some bacterial species of the genus Tenacibaculum, including Tenacibaculum ovolyticum, have been known as fish pathogens in the sea. So far, the only published genome sequence for this genus is for Tenacibaculum dicentrarchi, which could also be a fish pathogen. Strain da5A-8, showing 100% identity to the 16S rRNA gene sequence of T. ovolyticum DSM 18103T, was isolated from seawater at a depth of 344 m in Kochi, Japan, and grew optimally at 10 to 20°C. The genome sequence of strain da5A-8 revealed the possible virulence genes commonly observed in the genus Tenacibaculum. PMID:27365358

  15. L151-81A/B - A unique white dwarf binary with DB and DA components

    NASA Technical Reports Server (NTRS)

    Oswalt, Terry D.; Hintzen, Paul M.; Liebert, James W.; Sion, Edward M.

    1988-01-01

    Spectroscopic observations of the wide binary L151-81A/B reveal that both components are white dwarfs with spectral types DB3 and DA4, the first such binary identified in a comprehensive survey of Luyten and Giclas common proper motion pairs. Assuming log g = 8, measurements of the helium line profiles in the DB primary yield Teff = 16,000 + or - 2,000, while the hydrogen profiles for the DA companion yield Teff =12,000 + or - 2,000. The existence of a helium-rich/hydrogen-rich degenerate pair offers several interesting tests of theoretical white dwarf formation channels and surface abundance evolution.

  16. Purification, characterization and allergenicity assessment of 26kDa protein, a major allergen from Cicer arietinum.

    PubMed

    Verma, Alok Kumar; Sharma, Akanksha; Kumar, Sandeep; Gupta, Rinkesh Kumar; Kumar, Dinesh; Gupta, Kriti; Giridhar, B H; Das, Mukul; Dwivedi, Premendra D

    2016-06-01

    Chickpea (CP), a legume of the family Fabaceae, is an important nutrient-rich food providing protein, essential amino acids, vitamins, dietary fibre, and minerals. Unfortunately, several IgE-binding proteins in CP have been detected that are responsible for allergic manifestations in sensitized population. Therefore, the prevalence of CP induced allergy prompted us towards purification, characterization and allergenicity assessment of a major ∼26kDa protein from chickpea crude protein extract (CP-CPE). Purification of CP 26kDa protein was done using a combination of fractionation and anion exchange chromatography. This protein was further characterized as "Chain A, crystal structure of a plant albumin" from Cicer arietinum with Mol wt 25.8kDa by Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Further, allergenic potential of purified 25.8kDa protein was assessed using in vivo and in vitro model. Purified protein showed IgE-binding capacity with sensitized BALB/c mice and CP allergic patient's sera. Enhanced levels of specific and total IgE, MCP-1, MCPT-1, myeloperoxidase, histamine, prostaglandin D2, and cysteinyl leukotriene were found in sera of mice treated with CP ∼26kDa protein. Further, expressions of Th2 cytokines (i.e. IL-4, IL-5, IL-13), transcription factors (i.e. GATA-3, STAT-6, SOCS-3) and mast cell signaling proteins (Lyn, cFgr, Syk, PLC-γ2, PI-3K, PKC) were also found increased at mRNA and protein levels in the intestines of mice treated with CP ∼26kDa protein. In addition, enhanced release of β-hexosaminidase, histamine, cysteinyl leukotriene and prostaglandin D2 were observed in RBL2H3 cell line when treated (125μg) with CP 26kDa protein. Conclusively, in vivo and in vitro studies revealed the allergenic potential of purified CP 26kDa protein. Being a potential allergen, plant albumin may play a pivotal role in CP induced allergenicity. Current study will be helpful for better development of therapeutic approaches to

  17. Genome Sequence of the Psychrophilic Bacterium Tenacibaculum ovolyticum Strain da5A-8 Isolated from Deep Seawater.

    PubMed

    Teramoto, Maki; Zhai, Zhenyu; Komatsu, Ayumi; Shibayama, Keigo; Suzuki, Masato

    2016-01-01

    Some bacterial species of the genus Tenacibaculum, including Tenacibaculum ovolyticum, have been known as fish pathogens in the sea. So far, the only published genome sequence for this genus is for Tenacibaculum dicentrarchi, which could also be a fish pathogen. Strain da5A-8, showing 100% identity to the 16S rRNA gene sequence of T. ovolyticum DSM 18103(T), was isolated from seawater at a depth of 344 m in Kochi, Japan, and grew optimally at 10 to 20°C. The genome sequence of strain da5A-8 revealed the possible virulence genes commonly observed in the genus Tenacibaculum. PMID:27365358

  18. In para totale...una cosa da panico...sulla lingua dei giovani in Italia (In para totale...una cosa da panico...The Language of Young People in Italy).

    ERIC Educational Resources Information Center

    Marcato, Carla

    1997-01-01

    Describes and analyzes the language of young people in Italy today. Particular focus is on the expressions using "para" (e.g., "in para totale" = to be very bored or worried) and the phrase "una cosa da panico" (something terrible or its opposite something wonderful). (CFM)

  19. DaVIE: Database for the Visualization and Integration of Epigenetic data

    PubMed Central

    Fejes, Anthony P.; Jones, Meaghan J.; Kobor, Michael S.

    2014-01-01

    One of the challenges in the analysis of large data sets, particularly in a population-based setting, is the ability to perform comparisons across projects. This has to be done in such a way that the integrity of each individual project is maintained, while ensuring that the data are comparable across projects. These issues are beginning to be observed in human DNA methylation studies, as the Illumina 450k platform and next generation sequencing-based assays grow in popularity and decrease in price. This increase in productivity is enabling new insights into epigenetics, but also requires the development of pipelines and software capable of handling the large volumes of data. The specific problems inherent in creating a platform for the storage, comparison, integration, and visualization of DNA methylation data include data storage, algorithm efficiency and ability to interpret the results to derive biological meaning from them. Databases provide a ready-made solution to these issues, but as yet no tools exist that that leverage these advantages while providing an intuitive user interface for interpreting results in a genomic context. We have addressed this void by integrating a database to store DNA methylation data with a web interface to query and visualize the database and a set of libraries for more complex analysis. The resulting platform is called DaVIE: Database for the Visualization and Integration of Epigenetics data. DaVIE can use data culled from a variety of sources, and the web interface includes the ability to group samples by sub-type, compare multiple projects and visualize genomic features in relation to sites of interest. We have used DaVIE to identify patterns of DNA methylation in specific projects and across different projects, identify outlier samples, and cross-check differentially methylated CpG sites identified in specific projects across large numbers of samples. A demonstration server has been setup using GEO data at http

  20. Micromorphology of selected relict slope deposits from Serra da Estrela, Portugal

    NASA Astrophysics Data System (ADS)

    Nieuwendam, Alexandre; Vieira, Gonçalo; Schaefer, Carlos

    2013-04-01

    Serra da Estrela is the highest mountain in Portugal (1,993 m ASL) and part of the Iberian Central Cordillera. The mountain has a strong relief and a lithological diversity with several types of granitoids and metasediments. Most of the western plateau area was glaciated during the Last Glacial Maximum and its morphology is dominated by glacial landforms. Vieira (2004) produced a detailed geomorphological map of Serra da Estrela and described several sites showing stratified slope, head and debris-flow deposits. Based on the geomorphological analysis of the relationships between glacial and periglacial evidence, a first relative chronology was presented. However, a detailed and systematical sedimentological analysis has not been conducted before and absolute ages are also lacking. Micromorphology analysis has proven to be of considerable value in the interpretation of mountain soils and sediments. Such interpretation depends on identifying diagnostic features, indicating factors as the presence or absence of permafrost, thickness of the active layer, ice segregation and the operation of processes of mass-wasting. In this study, micromorphology was used to answer questions concerning the composition, structure, origin and depositional processes of relict slope deposits. Micromorphology allowed a systematic description of the physical characteristics of the sediments. Lamination and sorting, when preserved, are good evidence for overland flow. Features due to deformation (folds, boudins, coatings and tails due to the rotation of clasts) are associated with sliding. Other mass-movements such as debris flows, earth flows, and to a certain extent, dry grain flows may be characterized by similar microscopic facies, typically a poorly sorted, porphyric material. Porosity gives evidence for both liquefaction (debris flows) and frost-induced mass-movement (solifluction). The relict slope deposits of the Serra da Estrela show an increase in cryogenic micromorphological

  1. Detection of cohesive forces in the rubble-pile asteroid (29075) 1950 DA

    NASA Astrophysics Data System (ADS)

    Rozitis, Benjamin; MacLennan, Eric; Emery, Joshua P.

    2014-11-01

    The physical behavior of rubble-pile asteroids has traditionally been described using only gravitational and frictional forces within a granular material. Cohesive forces in the form of small van der Waals forces between constituent grains have recently been predicted to be important for small rubble-pile asteroids (less than 10 kilometers in size), and could potentially explain how small fast spinning asteroids remain intact. It is possible to infer the existence of cohesive forces within a rubble-pile asteroid by determining if it has insufficient self-gravity, dictated by its bulk density, to prevent rotational breakup by centrifugal forces. The kilometer-sized and potentially-hazardous asteroid (29075) 1950 DA is one of the largest known candidates for being held together by cohesive forces, as it has a rotation period of 2.1216 h that is just beyond the critical spin limit of 2.2 h estimated for a cohesionless asteroid. Using the Advanced Thermophysical Model (or ATPM), in combination with the radar shape model, WISE thermal-infrared data, and Yarkovsky orbital drift measurement, we determined the thermal inertia, bulk density, and cohesive strength of (29075) 1950 DA (Rozitis et al., 2014, Nature, 512, 174-176). The thermal inertia value is remarkably low at 24 +20/-14 SI units, which gives a corresponding bulk density of 1.7 ± 0.7 g/cm^3 in the Yarkovsky orbital drift analysis. This bulk density is typical of a rubble-pile asteroid, and a minimum cohesive strength of 64 +12/-20 Pa is therefore required to prevent surface mass shedding and structural failure by centrifugal forces. This strength is comparable to, though somewhat less than, the cohesive forces found between the grains of lunar regolith. Finally, as (29075) 1950 DA has a 1 in 19,800 chance of impacting the Earth in 2880, and has the potential to disrupt like main-belt comet P/2013 R3, it raises new implications for impact mitigation against fast spinning rubble-pile asteroids.

  2. Toxicokinetics of the phytoestrogen daidzein in female DA/Han rats.

    PubMed

    Janning, P; Schuhmacher, U S; Upmeier, A; Diel, P; Michna, H; Degen, G H; Bolt, H M

    2000-10-01

    Female DA/Han rats were given the phytoestrogen daidzein, either intravenously (10 mg/kg b.w.) or orally by gavage (10 or 100 mg/kg b.w.). The plasma concentration-time curve determined after i.v. administration of daidzein was fitted to a triexponential model, resulting in a final half-life (gamma-phase) of approximately 4 h. The oral bioavailability of 10 mg daidzein/kg was 9.7%, while that of 100 mg/kg was 2.2%; the higher dose (100 mg/kg) was apparently absorbed to a four- to fivefold lower extent than the smaller dose. The plasma concentration time curves after oral administration of daidzein to female DA/Han rats revealed pronounced interindividual differences and multiple peaks, pointing to extensive enterohepatic circulation and/or protracted absorption from the gastrointestinal tract. As shown in a separate experiment with bile duct-cannulated rats, daidzein (i.p. 10 mg/kg b.w.) is efficiently excreted with bile: glucuronide/sulfate metabolites amounting to approximately 30% of the dose in 8 h. Conjugates were also the main circulating metabolites upon i.v. or gavage administration of daidzein, indicating efficient phase II metabolism in female DA/Han rats. Since only few data have been published on tissue levels of isoflavones, their concentrations were measured in various organs and compared to plasma levels determined at the time the animals were killed, with one exception 32 or 48 h after rats had received a single dose of daidzein (i.v. or per os). As expected, the daidzein concentrations depended upon dose and administration route. Despite notable differences in the absolute amounts of total daidzein (free plus hydrolyzed conjugates), the levels were usually three- to fivefold higher in liver and kidney than in plasma; in most samples of uteri, the concentrations were similar, or up to twofold higher, than the respective plasma levels. These data point to an uptake and storage of isoflavones and metabolites in tissues. Experimental toxicokinetics

  3. Chlorophyll antenna proteins of photosystem I: topology, synthesis, and regulation of the 20-kDa subunit of Chlamydomonas light-harvesting complex of photosystem I

    SciTech Connect

    Herrin, D.L.; Plumley, F.G.; Ikeuchi, M.; Michaels, A.S.; Schmidt, G.W.

    1987-05-01

    The light-harvesting complex of photosystem I (LHCI) was isolated from wild-type cells of Chlamydomonas reinhardtii; the Chl a/b-protein complex contains four major polypeptides of approximately 27, 26, 24, and 20 kDa (polypeptides 14, 15, 17.2, and 22, respectively, in the nomenclature for Chlamydomonas thylakoid proteins). Antiserum against the 20-kDa subunit of LHCI was prepared and used to determine the membrane topology, subcellular site of synthesis, and cell-cycle regulation of this polypeptide. The results indicate that the 20-kDa subunit as well as the other major LHCI polypeptides are integral membrane proteins. Moreover, protease digestion experiments reveal that the 20-kDa polypeptide is completely protected by the membrane bilayer but the 27- and 26-kDa LHCI polypeptides are exposed at the membrane surface. In vivo synthesis of the 20-kDa polypeptide is sensitive to cycloheximide but not to chloramphenicol; the form of the polypeptide recovered from in vitro translations of polyadenylated RNA is approximately 24 kDa, 4 kDa larger than the mature polypeptide. It is concluded that this LHCI polypeptide is nuclear encoded and synthesized in the cytoplasm as a higher molecular weight precursor. Synthesis of the 20-kDa polypeptide is restricted to the light period in light-dark synchronized cells. Translatable mRNA for this polypeptide accumulates during the light but levels are dramatically reduced during the dark period. Thus, synthesis of the 20-kDa subunit of LHCI appears to be transcriptionally regulated during the cell cycle.

  4. Molecular cloning, tissue distribution, and expression of a 14-kDa bile acid-binding protein from rat ileal cytosol.

    PubMed Central

    Gong, Y Z; Everett, E T; Schwartz, D A; Norris, J S; Wilson, F A

    1994-01-01

    A cDNA clone encoding the major intestinal cytosolic 14-kDa bile acid-binding protein (14-kDa I-BABP) was isolated from a rat ileal lambda gt22A library following immunoscreening using a monospecific antiserum raised against a 14-kDa polypeptide found in the rat ileal cytosol. One clone of 516 bp encoded a 128-amino acid protein with a predicted molecular mass of 14,544 Da. The deduced amino acid sequence of 14-kDa I-BABP showed 100% homology to rat intestinal 15-kDa protein (I-15P) and 72% homology to porcine 15-kDa gastrotropin, whereas comparison of I-BABP to rat 14-kDa fatty acid-binding proteins of liver, intestine, and heart revealed homologies of 44%, 25%, and 28%, respectively. Northern blot analysis revealed a single transcript of approximately 0.5 kb in ileum and ovary; however, the abundance of I-BABP mRNA was much greater in ileum than in ovary. No transcript was seen in RNA extracted from stomach, jejunum, colon, liver, adrenal, brain, heart, kidney, or testis. Transfection of the I-BABP cDNA into COS-7 cells resulted in the expression of a 14-kDa protein that was identical to the ileal cytosolic I-BABP as determined by immunoblotting. Photoaffinity labeling of expressed 14-kDa protein was saturable with respect to increasing concentrations of 7,7-azo[3H]taurocholate (Km, 83.3 microM; Vmax, 6.7 pmol/mg per 5 min). Taurocholate inhibited 7,7-azotaurocholate labeling by > 96% with lesser inhibition by taurochenodeoxycholate (83.1%), chenodeoxycholate (74.6%), cholate (50.5%), and progesterone (38.5%), whereas oleic acid and estradiol did not inhibit binding. Images PMID:8197128

  5. Chlorophyll antenna proteins of photosystem I: topology, synthesis, and regulation of the 20-kDa subunit of Chlamydomonas light-harvesting complex of photosystem I.

    PubMed

    Herrin, D L; Plumley, F G; Ikeuchi, M; Michaels, A S; Schmidt, G W

    1987-05-01

    The light-harvesting complex of photosystem I (LHCI) was isolated from wild-type cells of Chlamydomonas reinhardtii; the Chl a/b-protein complex contains four major polypeptides of approximately 27, 26, 24, and 20 kDa (polypeptides 14, 15, 17.2, and 22, respectively, in the nomenclature for Chlamydomonas thylakoid proteins). Antiserum against the 20-kDa subunit of LHCI was prepared and used to determine the membrane topology, subcellular site of synthesis, and cell-cycle regulation of this polypeptide. The results indicate that the 20-kDa subunit as well as the other major LHCI polypeptides are integral membrane proteins. Moreover, protease digestion experiments reveal that the 20-kDa polypeptide is completely protected by the membrane bilayer but the 27- and 26-kDa LHCI polypeptides are exposed at the membrane surface. In vivo synthesis of the 20-kDa polypeptide is sensitive to cycloheximide but not to chloramphenicol; the form of the polypeptide recovered from in vitro translations of polyadenylated RNA is approximately 24 kDa, 4 kDa larger than the mature polypeptide. It is concluded that this LHCI polypeptide is nuclear encoded and synthesized in the cytoplasm as a higher molecular weight precursor. Synthesis of the 20-kDa polypeptide is restricted to the light period in light-dark synchronized cells. Translatable mRNA for this polypeptide accumulates during the light but levels are dramatically reduced during the dark period. Thus, synthesis of the 20-kDa subunit of LHCI appears to be transcriptionally regulated during the cell cycle. PMID:3555343

  6. Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants.

    PubMed

    Byun, Mi Young; Lee, Jungeun; Cui, Li Hua; Kang, Yoonjee; Oh, Tae Kyung; Park, Hyun; Lee, Hyoungseok; Kim, Woo Taek

    2015-07-01

    Deschampsia antarctica is an Antarctic hairgrass that grows on the west coast of the Antarctic peninsula. In this report, we have identified and characterized a transcription factor, D. antarctica C-repeat binding factor 7 (DaCBF7), that is a member of the monocot group V CBF homologs. The protein contains a single AP2 domain, a putative nuclear localization signal, and the typical CBF signature. DaCBF7, like other monocot group V homologs, contains a distinct polypeptide stretch composed of 43 amino acids in front of the AP2 motif. DaCBF7 was predominantly localized to nuclei and interacted with the C-repeat/dehydration responsive element (CRT/DRE) core sequence (ACCGAC) in vitro. DaCBF7 was induced by abiotic stresses, including drought, cold, and salinity. To investigate its possible cellular role in cold tolerance, a transgenic rice system was employed. DaCBF7-overexpressing transgenic rice plants (Ubi:DaCBF7) exhibited markedly increased tolerance to cold stress compared to wild-type plants without growth defects; however, overexpression of DaCBF7 exerted little effect on tolerance to drought or salt stress. Transcriptome analysis of a Ubi:DaCBF7 transgenic line revealed 13 genes that were up-regulated in DaCBF7-overexpressing plants compared to wild-type plants in the absence of cold stress and in short- or long-term cold stress. Five of these genes, dehydrin, remorin, Os03g63870, Os11g34790, and Os10g22630, contained putative CRT/DRE or low-temperature responsive elements in their promoter regions. These results suggest that overexpression of DaCBF7 directly and indirectly induces diverse genes in transgenic rice plants and confers enhanced tolerance to cold stress. PMID:26025521

  7. Test of ''Crab-Waist'' Collisions at the DA{Phi}NE {Phi} Factory

    SciTech Connect

    Zobov, M.; Alesini, D.; Biagini, M. E.; Biscari, C.; Bocci, A.; Boni, R.; Boscolo, M.; Bossi, F.; Buonomo, B.; Clozza, A.; Delle Monache, G. O.; Demma, T.; Di Pasquale, E.; Di Pirro, G.; Drago, A.; Gallo, A.; Ghigo, A.; Guiducci, S.; Ligi, C.; Marcellini, F.

    2010-04-30

    The electron-positron collider DA{Phi}NE, the Italian {Phi} factory, has been recently upgraded in order to implement an innovative collision scheme based on large crossing angle, small beam sizes at the crossing point, and compensation of beam-beam interaction by means of sextupole pairs creating a ''crab-waist'' configuration in the interaction region. Experimental tests of the novel scheme exhibited an increase by a factor of 3 in the peak luminosity of the collider with respect to the performances reached before the upgrade. In this Letter we present the new collision scheme, discuss its advantages, describe the hardware modifications realized for the upgrade, and report the results of the experimental tests carried out during commissioning of the machine in the new configuration and standard operation for the users.

  8. Toward a Network of Faint DA White Dwarfs as High-precision Spectrophotometric Standards

    NASA Astrophysics Data System (ADS)

    Narayan, G.; Axelrod, T.; Holberg, J. B.; Matheson, T.; Saha, A.; Olszewski, E.; Claver, J.; Stubbs, C. W.; Bohlin, R. C.; Deustua, S.; Rest, A.

    2016-05-01

    We present the initial results from a program aimed at establishing a network of hot DA white dwarfs to serve as spectrophotometric standards for present and future wide-field surveys. These stars span the equatorial zone and are faint enough to be conveniently observed throughout the year with large-aperture telescopes. The spectra of these white dwarfs are analyzed in order to generate a non-local-thermodynamic-equilibrium model atmosphere normalized to Hubble Space Telescope colors, including adjustments for wavelength-dependent interstellar extinction. Once established, this standard star network will serve ground-based observatories in both hemispheres as well as space-based instrumentation from the UV to the near IR. We demonstrate the effectiveness of this concept and show how two different approaches to the problem using somewhat different assumptions produce equivalent results. We discuss the lessons learned and the resulting corrective actions applied to our program.

  9. The binary Feige 24 - The mass, radius, and gravitational redshift of the DA white dwarf

    NASA Technical Reports Server (NTRS)

    Vennes, Stephane; Shipman, Harry L.; Thorstensen, John R.; Thejll, Peter

    1991-01-01

    Observations are reported which refine the binary ephemeris of the Feige 24 system, which contains a peculiar hot DA white dwarf and an M dwarf with an atmosphere illuminated by extreme ultraviolet radiation from the white dwarf. With the new ephemeris and a set of IUE high-dispersion spectra, showing phase-dependent redshifted C IV, N V, and Si IV resonance lines, the orbital velocity, and hence the mass (0.54 + or - 0.20 solar masses), and the gravitational redshift of the white dwarf (14.1 + or - 5.2 km/s) are determined independently. It is shown that the measured Einstein redshift is consistent with an estimated radius for the white dwarf obtained from a model atmosphere solid angle and a parallax measurement. This radius is twice the Hamada-Salpeter radius for the given mass and offers a prospect to investigate the presence of a massive hydrogen envelope in that white dwarf star.

  10. THE KEPLER LIGHT CURVE OF THE UNIQUE DA WHITE DWARF BOKS 53856

    SciTech Connect

    Holberg, J. B.; Howell, Steve B. E-mail: howell@noao.edu

    2011-08-15

    The faint (g = 16.9) hot white dwarf BOKS 53856 was observed by the Kepler Mission in short cadence mode during mid-2009. Analysis of these observations reveals a highly stable modulation with a period of 6.1375 hr and a 2.46% half-amplitude. The folded light curve has an unusual shape that is difficult to explain in terms of a binary system containing an unseen companion more luminous than an L0 brown dwarf. Optical spectra of BOKS 53856 show a T{sub eff} = 34,000 K, log g = 8.0 DA white dwarf. There are few, if any, known white dwarfs in this temperature range exhibiting photometric variations similar to those we describe. A magnetic spin-modulated white dwarf model can in principle explain the light curve, an interpretation supported by spectral observations of the H{alpha} line showing evidence of Zeeman splitting.

  11. Sensitivity of EIA in the diagnosis of tuberculosis using 38-kDa antigen.

    PubMed

    Chander, J; Subrahmanyan, S; Gupta, R

    1996-10-01

    Patients, who reported to Government Medical College Hospital, Chandigarh with suspected signs and symptoms of pulmonary tuberculosis, were examined. Their sputum was investigated for the presence of acid-fast bacilli, using the routine Ziehl-Neelsen staining, for 3 consecutive days. The positive cases were referred for treatment and the rest were referred for supplementary tests like x-ray, Mantoux test, FNAC and the presence of IgG antibodies in their serum against recombinant 38-kDa antigen derived from Mycobacterium tuberculosis, using micro-ELISA plates. It was found that 37.8% of the cases who were negative for acid-fast bacilli found to be positive for the presence of antimycobacterial IgG antibodies with an antibody titre greater than the cut off value in addition to one of the above mentioned positive supplementary finding. Hence, EIA for tuberculosis is more sensitive as compared to the routine acid-fast bacilli staining. PMID:9141876

  12. First Results of the VLBI Experiment on Radar Location of the Asteroid 2012 DA14

    NASA Astrophysics Data System (ADS)

    Nechaeva, M.; Antipenko, A.; Bezrukov, D.; Bezrukovs, Vl.; Dementjev, A.; Dugin, N.; Jekabsons, N.; Khutornoy, R.; Klapers, M.; Konovalenko, A.; Kulishenko, V.; Nabatov, A.; Nesteruk, V.; Pupillo, G.; Reznichenko, A.; Salerno, E.; Shmeld, I.; Skirmante, K.; Tikhomirov, Yu.; Voytyuk, V.

    An international VLBI experiment on radio location of the asteroid 2012 DA14 was organized on 2013 February 15--16, during its flyby close to Earth. The purpose of observations was to investigate and specify orbital parameters of the asteroid, as well as to evaluate its rotation period and other characteristics. The irradiation of the asteroid was performed by the RT-70 transmitter at Evpatoria (Crimea, Ukraine), while the reflected signals were successfully accepted by the two 32 m radio telescopes at Medicina (Bologna, Italy) and Irbene (Ventspils, Latvia). Processing and interpretation of the data were performed both in the Radiophysical Research Institute and in the Ventspils International Radio Astronomy Center. The first results of this experiment are presented and discussed.

  13. Shadowing and absorption effects on J/psi production in dA collisions

    SciTech Connect

    Vogt, R.

    2004-11-01

    The authors study medium modifications of J/{psi} production in cold nuclear media in deuterium-nucleus collisions. They discuss several parameterizations of the modifications of the parton densities in the nucleus, known as shadowing, an initial-state effect. They also include absorption of the produced J/{psi} by nucleons, a final-state effect. Both spatially homogeneous and inhomogeneous shadowing and absorption are considered. They use the number of binary nucleon-nucleon collisions as a centrality measure. Results are presented for d+Au collisions at {radical}S{sub NN} = 200 GeV and for d+Pb collisions at {radical}S{sub NN} = 6.2 TeV. To contrast the centrality dependence in pA and dA collisions, they also present pPb results at {radical}S{sub NN} = 8.8 TeV.

  14. A 138-kDa glycoprotein from Dictyostelium membranes with folate deaminase and folate binding activity.

    PubMed

    Greiner, R A; Jacobs-Krahnen, D; Mutzel, R; Malchow, D; Wurster, B

    1992-03-15

    A 138-kDa glycoprotein comprising folate deaminase activity was purified to apparent homogeneity from membranes of Dictyostelium discoideum. Deaminase activity could be effectively inhibited by p-chloromercuriphenylsulfonate. This treatment protected folate from deamination and thus allowed investigation of folate binding to deaminase fractions. Two types of folate binding sites, differing in affinity and specificity, were detected on the folate deaminase glycoprotein. One type displays high affinity and binds folate stronger than N10-methylfolate. This binding site appears to be identical with the catalytic site of folate deaminase. The other type of binding site shows lower affinity but prefers N10-methylfolate relative to folate. A similar preference for N10-methylfolate was observed in chemotaxis tests pointing to the possibility that the second type of binding site is involved in chemotactic perception of folate compounds. Folate perception and deamination could thus be performed by activities residing on the same polypeptide. PMID:1544893

  15. Preliminary XCOV26 Results For The Da White Dwarf EC14012-1446

    NASA Astrophysics Data System (ADS)

    Provencal, Judith L.; Thompson, S.; Montgomery, M.; Shipman, H.; Dalessio, J.; Whole Earth Telescope Team

    2010-01-01

    EC14012-1446 is a hydrogen atmosphere (DA) white dwarf pulsator. Its rich pulsation spectrum displays a range of excited modes with complex multiplet structure, in addition to numerous combination frequencies. In April 2008, EC14012-1446 was the primary target of XCOV26. We obtained over 300 hrs of nearly continuous high speed photometry with the goal of using the nonlinear pulse shapes to empirically determine the parameters of the convection zone. The Fourier transform (FT) of the light curve contains power between 1000 to 4000 microHz, with the dominant peak at 1234 microHz. We find 13 independent frequencies distributed in 8 modes, as well as a myriad of combination frequencies. We present preliminary results of our asteroseismological investigation and lay the groundwork for future investigation leading to light curve fitting of EC14012-1446. This research is funded by Mt. Cuba Observatory (Greenville, DE) and the Crystal Trust Foundation.

  16. Middle Paleolithic human remains from the Gruta Da Oliveira (Torres Novas), Portugal.

    PubMed

    Willman, John C; Maki, Julia; Bayle, Priscilla; Trinkaus, Erik; Zilhão, João

    2012-09-01

    Additional Middle Paleolithic human remains from layers 17, 18, and 22 of the Gruta da Oliveira, Portugal consist of a proximal manual phalanx 2 (Oliveira 5), a partial postcanine tooth (Oliveira 6), a humeral diaphysis (Oliveira 7), a distal mandibular molar (Oliveira 8), and a mandibular premolar (P(3) ) (Oliveira 9). Oliveira 5, 6, and 8 are unremarkable for Late Pleistocene humans. The Oliveira 7 right humerus is moderately robust or the individual had the stocky body proportions of other European (including Iberian) Neandertals. The Oliveira 9 P(3) has a large and symmetrical crown and lacks a distal accessory ridge and accessory lingual cusps, overlapping both Neandertal and recent human ranges of variation. It contrasts with at least recent human P(3) s in having relatively thin enamel. These join the Oliveira 1 to 4 remains in further documenting early MIS 3 Neandertal morphology in western Iberia. PMID:22610966

  17. A NEW TIMESCALE FOR PERIOD CHANGE IN THE PULSATING DA WHITE DWARF WD 0111+0018

    SciTech Connect

    Hermes, J. J.; Montgomery, M. H.; Winget, D. E.; Mullally, Fergal; Bischoff-Kim, A.

    2013-03-20

    We report the most rapid rate of period change measured to date for a pulsating DA (hydrogen atmosphere) white dwarf (WD), observed in the 292.9 s mode of WD 0111+0018. The observed period change, faster than 10{sup -12} s s{sup -1}, exceeds by more than two orders of magnitude the expected rate from cooling alone for this class of slow and simply evolving pulsating WDs. This result indicates the presence of an additional timescale for period evolution in these pulsating objects. We also measure the rates of period change of nonlinear combination frequencies and show that they share the evolutionary characteristics of their parent modes, confirming that these combination frequencies are not independent modes but rather artifacts of some nonlinear distortion in the outer layers of the star.

  18. Basil as functional and preserving ingredient in "Serra da Estrela" cheese.

    PubMed

    Carocho, Márcio; Barros, Lillian; Barreira, João C M; Calhelha, Ricardo C; Soković, Marina; Fernández-Ruiz, Virginia; Buelga, Celestino Santos; Morales, Patricia; Ferreira, Isabel C F R

    2016-09-15

    Antitumor, antimicrobial and antioxidant activities of basil were studied, along with its characterization in phenolic compounds, organic acids and soluble sugars. The results placed basil as a valuable candidate for functionalization and conservation of food products, maintaining their nutritional properties, while increasing their shelf life and potential health effects. The basil leaves were then incorporated in "Serra da Estrela Cheese", either in its dehydrated form or as a decoction. The cheeses were then subject to a nutritional evaluation, being characterized for their fatty acids, minerals and CIE color parameters. To assess the combined effects of plant incorporation and storage time, a 2-way ANOVA was used to process the results, further analysed through a linear discriminant analysis. Overall, basil leaves provided antioxidant activity to the cheeses, reduced the moisture, and preserved the unsaturated fatty acids and proteins. Comparing both incorporation types, the decoctions had a higher functionalizing and conservative effect. PMID:27080879

  19. Application of da Vinci® Robot in simple or radical hysterectomy: Tips and tricks

    PubMed Central

    Iavazzo, Christos; Gkegkes, Ioannis D.

    2016-01-01

    The first robotic simple hysterectomy was performed more than 10 years ago. These days, robotic-assisted hysterectomy is accepted as an alternative surgical approach and is applied both in benign and malignant surgical entities. The two important points that should be taken into account to optimize postoperative outcomes in the early period of a surgeon’s training are how to achieve optimal oncological and functional results. Overcoming any technical challenge, as with any innovative surgical method, leads to an improved surgical operation timewise as well as for patients’ safety. The standardization of the technique and recognition of critical anatomical landmarks are essential for optimal oncological and clinical outcomes on both simple and radical robotic-assisted hysterectomy. Based on our experience, our intention is to present user-friendly tips and tricks to optimize the application of a da Vinci® robot in simple or radical hysterectomies. PMID:27403078

  20. Microbiological Analysis of Surfaces of Leonardo Da Vinci's Atlantic Codex: Biodeterioration Risk

    PubMed Central

    Moroni, Catia; Pasquariello, Giovanna; Maggi, Oriana

    2014-01-01

    Following the discovery of discoloration on some pages of the Atlantic Codex (AC) of Leonardo da Vinci kept in the Biblioteca Ambrosiana in Milan, some investigations have been carried out to verify the presence of microorganisms, such as bacteria and fungi. To verify the presence of microorganisms a noninvasive method of sampling has been used that was efficient and allowed us to highlight the microbial facies of the material that was examined using conventional microbiological techniques. The microclimatic conditions in the storage room as well as the water content of the volume were also assessed. The combined observations allowed the conclusion that the discoloration of suspected biological origin on some pages of AC is not related to the presence or current attack of microbial agents. PMID:25574171