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Sample records for sea anemones cnidaria

  1. Latitudinal diversity of sea anemones (Cnidaria: Actiniaria).

    PubMed

    Fautin, Daphne Gail; Malarky, Lacey; Soberón, Jorge

    2013-04-01

    We sought to determine if the global distribution of sea anemones (cnidarian order Actiniaria) conforms to the classic pattern of biogeography--taxon richness at the equator with attenuation toward the poles--a pattern that is derived almost entirely from data on terrestrial plants and animals. We plotted the empirical distribution of species occurrences in 10° bands of latitude based on published information, then, using the Chao2 statistic, inferred the completeness of that inventory. We found the greatest species richness of sea anemones at 30-40° N and S, with lower numbers at tropical latitudes and the fewest species in polar areas. The Chao2 statistic allowed us to infer that the richness pattern we found is not due to particularly poor knowledge of tropical sea anemones. No 10° band of latitude has less than 60% of the theoretical number of species known, but for only about half of them could we reject the null hypothesis (P = 0.05) that information is complete; anemone diversity is best documented at high latitudes. We infer that the 1089 valid species currently known constitute about 70% of the theoretical total of about 1500 species of Actiniaria. The distribution pattern of sea anemone species resembles that of planktonic foraminiferans and benthic marine algae, although planktonic bacteria, marine bivalves, and shallow and deep scleractinian corals show the terrestrial pattern of equatorial richness attenuating with latitude. Sea anemone species richness is complementary to that of scleractinian corals at many scales; our findings affirm it at the global scale. PMID:23677974

  2. Sea Anemone (Cnidaria, Anthozoa, Actiniaria) Toxins: An Overview

    PubMed Central

    Frazão, Bárbara; Vasconcelos, Vitor; Antunes, Agostinho

    2012-01-01

    The Cnidaria phylum includes organisms that are among the most venomous animals. The Anthozoa class includes sea anemones, hard corals, soft corals and sea pens. The composition of cnidarian venoms is not known in detail, but they appear to contain a variety of compounds. Currently around 250 of those compounds have been identified (peptides, proteins, enzymes and proteinase inhibitors) and non-proteinaceous substances (purines, quaternary ammonium compounds, biogenic amines and betaines), but very few genes encoding toxins were described and only a few related protein three-dimensional structures are available. Toxins are used for prey acquisition, but also to deter potential predators (with neurotoxicity and cardiotoxicity effects) and even to fight territorial disputes. Cnidaria toxins have been identified on the nematocysts located on the tentacles, acrorhagi and acontia, and in the mucous coat that covers the animal body. Sea anemone toxins comprise mainly proteins and peptides that are cytolytic or neurotoxic with its potency varying with the structure and site of action and are efficient in targeting different animals, such as insects, crustaceans and vertebrates. Sea anemones toxins include voltage-gated Na+ and K+ channels toxins, acid-sensing ion channel toxins, Cytolysins, toxins with Kunitz-type protease inhibitors activity and toxins with Phospholipase A2 activity. In this review we assessed the phylogentic relationships of sea anemone toxins, characterized such toxins, the genes encoding them and the toxins three-dimensional structures, further providing a state-of-the-art description of the procedures involved in the isolation and purification of bioactive toxins. PMID:23015776

  3. Environmental sensing and response genes in Cnidaria: the chemical defensome in the sea anemone Nematostella vectensis

    PubMed Central

    Goldstone, J.V.

    2010-01-01

    The starlet sea anemone Nematostella vectensis has been recently established as a new model system for the study of the evolution of developmental processes, as cnidaria occupy a key evolutionary position at the base of the bilateria. Cnidaria play important roles in estuarine and reef communities, but are exposed to many environmental stressors. Here I describe the genetic components of a ‘chemical defensome’ in the genome of N. vectensis, and review cnidarian molecular toxicology. Gene families that defend against chemical stressors and the transcription factors that regulate these genes have been termed a ‘chemical defensome,’ and include the cytochromes P450 and other oxidases, various conjugating enyzymes, the ATP-dependent efflux transporters, oxidative detoxification proteins, as well as various transcription factors. These genes account for about 1% (266/27200) of the predicted genes in the sea anemone genome, similar to the proportion observed in tunicates and humans, but lower than that observed in sea urchins. While there are comparable numbers of stress-response genes, the stress sensor genes appear to be reduced in N. vectensis relative to many model protostomes and deuterostomes. Cnidarian toxicology is understudied, especially given the important ecological roles of many cnidarian species. New genomic resources should stimulate the study of chemical stress sensing and response mechanisms in cnidaria, and allow us to further illuminate the evolution of chemical defense gene networks. PMID:18956243

  4. Homeoboxes in sea anemones (Cnidaria:Anthozoa): a PCR-based survey of Nematostella vectensis and Metridium senile.

    PubMed

    Finnerty, J R; Martindale, M Q

    1997-08-01

    Homeobox genes belong to a phylogenetically widespread family of regulatory genes that play important roles in pattern formation and cell-fate specification in several model systems (e.g., Drosophila, mouse, and C. elegans). Although the evolution of many classes of homeobox genes predates the diversification of the Bilateria, comparatively little is known about homeobox genes in outgroups to the Bilateria, such as the Cnidaria. We used the polymerase chain reaction to recover 12 partial homeoboxes from 2 species of sea anemones, Metridium senile and Nematostella vectensis (phylum Cnidaria; class Anthozoa). These homeoboxes appear to represent 9 distinct, mutually paralogous homeobox genes, 5 of which belong to previously identified cnidarian homeobox classes, and 4 of which appear to represent previously unidentified classes. The evolutionary relationships between the homeodomains of sea anemones and of bilaterian animals were assessed through database searches and phylogenetic analyses. As many as 5 of the anemone homeoboxes may belong to the Hox class, which suggests that the Hox gene complement of cnidarians is larger than previously expected. Homologs of the even-skipped gene of Drosophila were also identified in both Metridium and Nematostella. PMID:9290214

  5. Sea anemones (Cnidaria, Anthozoa, Actiniaria) from coral reefs in the southern Gulf of Mexico

    PubMed Central

    González-Muñoz, Ricardo; Simões, Nuno; Tello-Musi, José Luis; Rodríguez, Estefanía

    2013-01-01

    Abstract Seven sea anemone species from coral reefs in the southern Gulf of Mexico are taxonomically diagnosed and images from living specimens including external and internal features, and cnidae are provided. Furthermore, the known distribution ranges from another 10 species are extended. No species records of sea anemones have been previously published in the primary scientific literature for coral reefs in the southern Gulf of Mexico and thus, this study represents the first inventory for the local actiniarian fauna. PMID:24146599

  6. Distribution of sea anemones (Cnidaria, Actiniaria) in Korea analyzed by environmental clustering

    USGS Publications Warehouse

    Cha, H.-R.; Buddemeier, R.W.; Fautin, D.G.; Sandhei, P.

    2004-01-01

    Using environmental data and the geospatial clustering tools LOICZView and DISCO, we empirically tested the postulated existence and boundaries of four biogeographic regions in the southern part of the Korean peninsula. Environmental variables used included wind speed, sea surface temperature (SST), salinity, tidal amplitude, and the chlorophyll spectral signal. Our analysis confirmed the existence of four biogeographic regions, but the details of the borders between them differ from those previously postulated. Specimen-level distribution records of intertidal sea anemones were mapped; their distribution relative to the environmental data supported the importance of the environmental parameters we selected in defining suitable habitats. From the geographic coincidence between anemone distribution and the clusters based on environmental variables, we infer that geospatial clustering has the power to delimit ranges for marine organisms within relatively small geographical areas.

  7. Edwardsia sojabio sp. n. (Cnidaria: Anthozoa: Actiniaria: Edwardsiidae), a new abyssal sea anemone from the Sea ofJapan

    NASA Astrophysics Data System (ADS)

    Sanamyan, Nadya; Sanamyan, Karen

    2013-02-01

    The paper describes new deep-water edwardsiid sea anemone Edwardsia sojabio sp. n. which is very common on soft muddy bottoms at lower bathyal and upper abyssal depths in the Sea of Japan. It was recorded in high quantity in depths between 2545 and 3550 m and is the second abyssal species of the genus Edwardsia.

  8. Patterns of Symbiodinium spp. associations within the family Aiptasiidae, a monophyletic lineage of symbiotic of sea anemones (Cnidaria, Actiniaria)

    NASA Astrophysics Data System (ADS)

    Grajales, Alejandro; Rodríguez, Estefanía; Thornhill, Daniel J.

    2016-03-01

    Although the symbiotic relationships between dinoflagellates and cnidarians are well recognized, few studies have examined these associations from an evolutionary perspective. This is especially true for symbiotic sea anemones, in which many reports consist of an approximate species identification of the host, followed by the identification of the dinoflagellate symbiont using molecular genetic markers. To further explore the evolutionary history of sea anemone-dinoflagellate associations, we documented the diversity of Symbiodinium spp. in a monophyletic clade of sea anemones, the family Aiptasiidae. We combined information from several molecular genetic markers, including nuclear ITS2 and plastid cp23S-rDNA, to evaluate the patterns of evolution and diversification of Symbiodinium in the light of an existing phylogenetic framework for the sea anemone host. At the host family level, we found no evidence for coevolution or reciprocal phylogenies between host and endosymbiont. However, within some individual host species, Symbiodinium spp. exhibited patterns of host specialization and cladogenesis. This pattern suggests that coevolution between host and symbiont occurred within species and genera lineages, but that this process was regularly disrupted and symbiotic partners were recombined during the longer-term evolutionary history of the Aiptasiidae. Furthermore, we observed independent cases of phylogeographical partitioning of Symbiodinium within a single host species, suggesting that ecological speciation along an environmental gradient contributed to the diversity of associations found in nature.

  9. Stylobates birtlesi sp. n., a new species of carcinoecium-forming sea anemone (Cnidaria, Actiniaria, Actiniidae) from eastern Australia

    PubMed Central

    Crowther, Andrea L.; Fautin, Daphne G.; Wallace, Carden C.

    2011-01-01

    Abstract We describe a new species of carcinoecium-forming sea anemone, Stylobates birtlesi sp. n., from sites 590–964 m deep in the Coral Sea, off the coast of Queensland, Australia. An anemone of this genus settles on a gastropod shell inhabited by a hermit crab, then covers and extends the shell to produce a chitinous structure termed a carcinoecium. Stylobates birtlesi sp. n. is symbiotic with the hermit crab Sympagurus trispinosus (Balss, 1911). The nature of marginal sphincter muscle and nematocyst size and distribution distinguish Stylobates birtlesi sp. n. from other species in the genus. The four known species of Stylobates are allopatric, each inhabiting a separate ocean basin of the Indo-West Pacific. We also extend the known range of Stylobates loisetteae in the Indian Ocean off the coast of Western Australia. PMID:21594082

  10. Hidden among Sea Anemones: The First Comprehensive Phylogenetic Reconstruction of the Order Actiniaria (Cnidaria, Anthozoa, Hexacorallia) Reveals a Novel Group of Hexacorals

    PubMed Central

    Rodríguez, Estefanía; Barbeitos, Marcos S.; Brugler, Mercer R.; Crowley, Louise M.; Grajales, Alejandro; Gusmão, Luciana; Häussermann, Verena; Reft, Abigail; Daly, Marymegan

    2014-01-01

    Sea anemones (order Actiniaria) are among the most diverse and successful members of the anthozoan subclass Hexacorallia, occupying benthic marine habitats across all depths and latitudes. Actiniaria comprises approximately 1,200 species of solitary and skeleton-less polyps and lacks any anatomical synapomorphy. Although monophyly is anticipated based on higher-level molecular phylogenies of Cnidaria, to date, monophyly has not been explicitly tested and at least some hypotheses on the diversification of Hexacorallia have suggested that actiniarians are para- or poly-phyletic. Published phylogenies have demonstrated the inadequacy of existing morphological-based classifications within Actiniaria. Superfamilial groups and most families and genera that have been rigorously studied are not monophyletic, indicating conflict with the current hierarchical classification. We test the monophyly of Actiniaria using two nuclear and three mitochondrial genes with multiple analytical methods. These analyses are the first to include representatives of all three currently-recognized suborders within Actiniaria. We do not recover Actiniaria as a monophyletic clade: the deep-sea anemone Boloceroides daphneae, previously included within the infraorder Boloceroidaria, is resolved outside of Actiniaria in several of the analyses. We erect a new genus and family for B. daphneae, and rank this taxon incerti ordinis. Based on our comprehensive phylogeny, we propose a new formal higher-level classification for Actiniaria composed of only two suborders, Anenthemonae and Enthemonae. Suborder Anenthemonae includes actiniarians with a unique arrangement of mesenteries (members of Edwardsiidae and former suborder Endocoelantheae). Suborder Enthemonae includes actiniarians with the typical arrangement of mesenteries for actiniarians (members of former suborders Protantheae, Ptychodacteae, and Nynantheae and subgroups therein). We also erect subgroups within these two newly-erected suborders. Although some relationships among these newly-defined groups are still ambiguous, morphological and molecular results are consistent enough to proceed with a new higher-level classification and to discuss the putative functional and evolutionary significance of several morphological attributes within Actiniaria. PMID:24806477

  11. Sea Anemone: Investigations.

    ERIC Educational Resources Information Center

    Hunt, John D.

    1982-01-01

    Several investigations can be undertaken with live sea anemones. A sea anemone's feeding response, fighting power, color, and symbiotic relationships to other invertebrates (such as a marine hermit crab) can be investigated in the high school classroom. Background information and laboratory procedures are provided. (Author/JN)

  12. A new genus and species of isanthid sea anemone (Cnidaria: Anthozoa: Actiniaria) from Chilean Patagonia, Anthoparactis fossii n. gen. et sp

    NASA Astrophysics Data System (ADS)

    Häussermann, Verena; Rodríguez, Estefanía

    2014-09-01

    We describe a new genus and species of sea anemone from Chilean Patagonia. Anthoparactis fossii n. gen. et sp. adds another acontiate genus and species to the family Isanthidae Carlgren, 1938. Anthoparactis n. gen. differs from the other isanthid genera in having the same number of mesenteries distally and proximally, acontia with basitrichs only, and a column with verrucae distally. Anthoparactis fossii n. sp. differs from the most similar species, Isoparactis fionae Lauretta et al., 2013, in the number of cycles of mesenteries and tentacles, structures of the column, colour pattern of the oral disc, cnidae, and geographical distribution. Isanthidae now includes seven genera and 11 species.

  13. Neurotoxin localization to ectodermal gland cells uncovers an alternative mechanism of venom delivery in sea anemones

    PubMed Central

    Moran, Yehu; Genikhovich, Grigory; Gordon, Dalia; Wienkoop, Stefanie; Zenkert, Claudia; Özbek, Suat; Technau, Ulrich; Gurevitz, Michael

    2012-01-01

    Jellyfish, hydras, corals and sea anemones (phylum Cnidaria) are known for their venomous stinging cells, nematocytes, used for prey and defence. Here we show, however, that the potent Type I neurotoxin of the sea anemone Nematostella vectensis, Nv1, is confined to ectodermal gland cells rather than nematocytes. We demonstrate massive Nv1 secretion upon encounter with a crustacean prey. Concomitant discharge of nematocysts probably pierces the prey, expediting toxin penetration. Toxin efficiency in sea water is further demonstrated by the rapid paralysis of fish or crustacean larvae upon application of recombinant Nv1 into their medium. Analysis of other anemone species reveals that in Anthopleura elegantissima, Type I neurotoxins also appear in gland cells, whereas in the common species Anemonia viridis, Type I toxins are localized to both nematocytes and ectodermal gland cells. The nematocyte-based and gland cell-based envenomation mechanisms may reflect substantial differences in the ecology and feeding habits of sea anemone species. Overall, the immunolocalization of neurotoxins to gland cells changes the common view in the literature that sea anemone neurotoxins are produced and delivered only by stinging nematocytes, and raises the possibility that this toxin-secretion mechanism is an ancestral evolutionary state of the venom delivery machinery in sea anemones. PMID:22048953

  14. Neurotoxin localization to ectodermal gland cells uncovers an alternative mechanism of venom delivery in sea anemones.

    PubMed

    Moran, Yehu; Genikhovich, Grigory; Gordon, Dalia; Wienkoop, Stefanie; Zenkert, Claudia; Ozbek, Suat; Technau, Ulrich; Gurevitz, Michael

    2012-04-01

    Jellyfish, hydras, corals and sea anemones (phylum Cnidaria) are known for their venomous stinging cells, nematocytes, used for prey and defence. Here we show, however, that the potent Type I neurotoxin of the sea anemone Nematostella vectensis, Nv1, is confined to ectodermal gland cells rather than nematocytes. We demonstrate massive Nv1 secretion upon encounter with a crustacean prey. Concomitant discharge of nematocysts probably pierces the prey, expediting toxin penetration. Toxin efficiency in sea water is further demonstrated by the rapid paralysis of fish or crustacean larvae upon application of recombinant Nv1 into their medium. Analysis of other anemone species reveals that in Anthopleura elegantissima, Type I neurotoxins also appear in gland cells, whereas in the common species Anemonia viridis, Type I toxins are localized to both nematocytes and ectodermal gland cells. The nematocyte-based and gland cell-based envenomation mechanisms may reflect substantial differences in the ecology and feeding habits of sea anemone species. Overall, the immunolocalization of neurotoxins to gland cells changes the common view in the literature that sea anemone neurotoxins are produced and delivered only by stinging nematocytes, and raises the possibility that this toxin-secretion mechanism is an ancestral evolutionary state of the venom delivery machinery in sea anemones. PMID:22048953

  15. Comprehensive EST analysis of the symbiotic sea anemone, Anemonia viridis

    PubMed Central

    Sabourault, Cécile; Ganot, Philippe; Deleury, Emeline; Allemand, Denis; Furla, Paola

    2009-01-01

    Background Coral reef ecosystems are renowned for their diversity and beauty. Their immense ecological success is due to a symbiotic association between cnidarian hosts and unicellular dinoflagellate algae, known as zooxanthellae. These algae are photosynthetic and the cnidarian-zooxanthellae association is based on nutritional exchanges. Maintenance of such an intimate cellular partnership involves many crosstalks between the partners. To better characterize symbiotic relationships between a cnidarian host and its dinoflagellate symbionts, we conducted a large-scale EST study on a symbiotic sea anemone, Anemonia viridis, in which the two tissue layers (epiderm and gastroderm) can be easily separated. Results A single cDNA library was constructed from symbiotic tissue of sea anemones A. viridis in various environmental conditions (both normal and stressed). We generated 39,939 high quality ESTs, which were assembled into 14,504 unique sequences (UniSeqs). Sequences were analysed and sorted according to their putative origin (animal, algal or bacterial). We identified many new repeated elements in the 3'UTR of most animal genes, suggesting that these elements potentially have a biological role, especially with respect to gene expression regulation. We identified genes of animal origin that have no homolog in the non-symbiotic starlet sea anemone Nematostella vectensis genome, but in other symbiotic cnidarians, and may therefore be involved in the symbiosis relationship in A. viridis. Comparison of protein domain occurrence in A. viridis with that in N. vectensis demonstrated an increase in abundance of some molecular functions, such as protein binding or antioxidant activity, suggesting that these functions are essential for the symbiotic state and may be specific adaptations. Conclusion This large dataset of sequences provides a valuable resource for future studies on symbiotic interactions in Cnidaria. The comparison with the closest available genome, the sea anemone N. vectensis, as well as with EST datasets from other symbiotic cnidarians provided a set of candidate genes involved in symbiosis-related molecular crosstalks. Altogether, these results provide new molecular insights that could be used as a starting-point for further functional genomics studies. PMID:19627569

  16. Sea anemones possess dynamic mitogenome structures.

    PubMed

    Emblem, Åse; Okkenhaug, Siri; Weiss, Emily S; Denver, Dee R; Karlsen, Bård Ove; Moum, Truls; Johansen, Steinar D

    2014-06-01

    A notable feature of hexacoral mitogenomes is the presence of complex self-catalytic group I introns. We investigated mitogenome structural variations and evolutionary mechanisms in actiniarian sea anemones based on the complete mitogenome sequence of the cold-water sea anemone species Urticina eques, Bolocera tuediae, Hormathia digitata and Metridium senile, and two isolates of the sub-tropical Aiptasia pulchella. Whole genome sequencing at 50 times coverage of B. tuediae and H. digitata indicated low mtDNA copy number of per haploid nuclear genome and presence of rare haplotypes. A group I intron inserted in ND5 was found to host essential mitochondrial protein genes in all species, and an additional truncated copy of ND5 in B. tuediae. A second group I intron (inserted in COI) that contained a homing endonuclease gene (HEG) was present in all mtDNA examined. Different variants of HEGs were observed, and included expressed elements fused in-frame with upstream exons and free-standing HEGs embedded within the intron. A notable hallmark of HEGs was a high extent of overlap with ribozyme structural elements; the U. eques HEG overlapped with the entire intron. We reconstructed the evolutionary history of the COI intron from insertion at unoccupied cognate sites, through HEG degradation, to intron loss. We also identified a novel insertion element in U. eques that contained two expressed protein-coding genes. An evolutionary analysis of the sea anemone mtDNA genes revealed higher substitution rates in the HEG and the insertion sequence as compared to the other loci, indicating relaxed selective pressures in these elements. We conclude that sea anemone mitogenomes are surprisingly dynamic in structure despite the economical organization and low sequence mutation rate. PMID:24613805

  17. NF-?B is required for cnidocyte development in the sea anemone Nematostella vectensis.

    PubMed

    Wolenski, Francis S; Bradham, Cynthia A; Finnerty, John R; Gilmore, Thomas D

    2013-01-01

    The sea anemone Nematostella vectensis (Nv) is a leading model organism for the phylum Cnidaria, which includes anemones, corals, jellyfishes and hydras. A defining trait across this phylum is the cnidocyte, an ectodermal cell type with a variety of functions including defense, prey capture and environmental sensing. Herein, we show that the Nv-NF-?B transcription factor and its inhibitor Nv-I?B are expressed in a subset of cnidocytes in the body column of juvenile and adult anemones. The size and distribution of the Nv-NF-?B-positive cnidocytes suggest that they are in a subtype known as basitrichous haplonema cnidocytes. Nv-NF-?B is primarily cytoplasmic in cnidocytes in juvenile and adult animals, but is nuclear when first detected in the 30-h post-fertilization embryo. Morpholino-mediated knockdown of Nv-NF-?B expression results in greatly reduced cnidocyte formation in the 5 day-old animal. Taken together, these results indicate that NF-?B plays a key role in the development of the phylum-specific cnidocyte cell type in Nematostella, likely by nuclear Nv-NF-?B-dependent activation of genes required for cnidocyte development. PMID:23063796

  18. Characterizing the spatiotemporal expression of RNAs and proteins in the starlet sea anemone, Nematostella vectensis

    PubMed Central

    Wolenski, Francis S; Layden, Michael J; Martindale, Mark Q; Gilmore, Thomas D; Finnerty, John R

    2016-01-01

    In an effort to reconstruct the early evolution of animal genes and proteins, there is an increasing focus on basal animal lineages such as sponges, cnidarians, ctenophores and placozoans. Among the basal animals, the starlet sea anemone Nematostella vectensis (phylum Cnidaria) has emerged as a leading laboratory model organism partly because it is well suited to experimental techniques for monitoring and manipulating gene expression. Here we describe protocols adapted for use in Nematostella to characterize the expression of RNAs by in situ hybridization using either chromogenic or fluorescence immunohistochemistry (~1 week), as well as to characterize protein expression by whole-mount immunofluorescence (~3 d). We also provide a protocol for labeling cnidocytes (~3 h), the phylum-specific sensory-effector cell type that performs a variety of functions in cnidarians, including the delivery of their venomous sting. PMID:23579779

  19. Biochemical and Electrophysiological Characterization of Two Sea Anemone Type 1 Potassium Toxins from a Geographically Distant Population of Bunodosoma caissarum

    PubMed Central

    Orts, Diego J. B.; Peigneur, Steve; Madio, Bruno; Cassoli, Juliana S.; Montandon, Gabriela G.; Pimenta, Adriano M. C.; Bicudo, José E. P. W.; Freitas, José C.; Zaharenko, André J.; Tytgat, Jan

    2013-01-01

    Sea anemone (Cnidaria, Anthozoa) venom is an important source of bioactive compounds used as tools to study the pharmacology and structure-function of voltage-gated K+ channels (KV). These neurotoxins can be divided into four different types, according to their structure and mode of action. In this work, for the first time, two toxins were purified from the venom of Bunodosoma caissarum population from Saint Peter and Saint Paul Archipelago, Brazil. Sequence alignment and phylogenetic analysis reveals that BcsTx1 and BcsTx2 are the newest members of the sea anemone type 1 potassium channel toxins. Their functional characterization was performed by means of a wide electrophysiological screening on 12 different subtypes of KV channels (KV1.1–KV1.6; KV2.1; KV3.1; KV4.2; KV4.3; hERG and Shaker IR). BcsTx1 shows a high affinity for rKv1.2 over rKv1.6, hKv1.3, Shaker IR and rKv1.1, while Bcstx2 potently blocked rKv1.6 over hKv1.3, rKv1.1, Shaker IR and rKv1.2. Furthermore, we also report for the first time a venom composition and biological activity comparison between two geographically distant populations of sea anemones. PMID:23466933

  20. Free amino acid pool of a sea anemone: exposure and recovery after an oil spill. [Sea Anemones

    SciTech Connect

    Kasschau, M.R.; Howard, C.L.

    1984-07-01

    A number of laboratory studies on marine invertebrates have shown changes in free amino acid (FAA) pools in response to various pollutants. During a nineteen-month field study to determine the effects of natural environmental parameters on the FAA pools to the Gulf Coast sea anemone, Bunodosoma cavernata, an oil tanker collision occurred about 8 miles off Galveston Island. The initial spill from the tanker Burma Agate occurred on November 1, 1979 with large leakages continuing for several weeks. There was no visible sign of oil on the first collection date 13 days after the spill, but 11 days later the anemones were covered with an oil sheen. As a result of this natural exposure to the oil, the authors decided to monitor the sea anemones for changes in the FAA pool during the oil exposure and recovery period.

  1. Nutrient enrichment coupled with sedimentation favors sea anemones over corals.

    PubMed

    Liu, Pi-Jen; Hsin, Min-Chieh; Huang, Yen-Hsun; Fan, Tung-Yung; Meng, Pei-Jie; Lu, Chung-Cheng; Lin, Hsing-Juh

    2015-01-01

    Fine sediments, which account for the majority of total fluvial sediment flux, have been suggested to degrade coral reefs on a global scale. Furthermore, sediment impacts can be exacerbated by extreme rainfall events associated with global climate change and anthropogenic nutrient enrichment. We report the findings from a series of mesocosm experiments exploring the effects of short-term sedimentation and nutrient enrichment on the interactions between the hard coral Acropora muricata, the sea anemone Mesactinia ganesis, and the green macroalga Codium edule. Mesocosms were manipulated to simulate either unimpacted reefs or reefs exposed to elevated levels of fine sediments for 10 or 14 days to simulate the effects of heavy rainfall. The first and second experiments were aimed to examine the effects of inorganic and organic sediments, respectively. The third experiment was designed to examine the interactive effects of nutrient enrichment and elevated sediment loads. Neither inorganic nor organic sediment loadings significantly affected the physiological performance of the coral, but, importantly, did reduce its ability to compete with other organisms. Photosynthetic efficiencies of both the green macroalga and the sea anemone increased in response to both sediment loadings when they were simultaneously exposed to nutrient enrichment. While organic sediment loading increased the nitrogen content of the green macroalga in the first experiment, inorganic sediment loading increased its phosphorus content in the second experiment. The coral mortality due to sea anemones attack was significantly greater upon exposure to enriched levels of organic sediments and nutrients. Our findings suggest that the combined effects of short-term sedimentation and nutrient enrichment could cause replacement of corals by sea anemones on certain coral reefs. PMID:25897844

  2. Nutrient Enrichment Coupled with Sedimentation Favors Sea Anemones over Corals

    PubMed Central

    Liu, Pi-Jen; Hsin, Min-Chieh; Huang, Yen-Hsun; Fan, Tung-Yung; Meng, Pei-Jie; Lu, Chung-Cheng; Lin, Hsing-Juh

    2015-01-01

    Fine sediments, which account for the majority of total fluvial sediment flux, have been suggested to degrade coral reefs on a global scale. Furthermore, sediment impacts can be exacerbated by extreme rainfall events associated with global climate change and anthropogenic nutrient enrichment. We report the findings from a series of mesocosm experiments exploring the effects of short-term sedimentation and nutrient enrichment on the interactions between the hard coral Acropora muricata, the sea anemone Mesactinia ganesis, and the green macroalga Codium edule. Mesocosms were manipulated to simulate either unimpacted reefs or reefs exposed to elevated levels of fine sediments for 10 or 14 days to simulate the effects of heavy rainfall. The first and second experiments were aimed to examine the effects of inorganic and organic sediments, respectively. The third experiment was designed to examine the interactive effects of nutrient enrichment and elevated sediment loads. Neither inorganic nor organic sediment loadings significantly affected the physiological performance of the coral, but, importantly, did reduce its ability to compete with other organisms. Photosynthetic efficiencies of both the green macroalga and the sea anemone increased in response to both sediment loadings when they were simultaneously exposed to nutrient enrichment. While organic sediment loading increased the nitrogen content of the green macroalga in the first experiment, inorganic sediment loading increased its phosphorus content in the second experiment. The coral mortality due to sea anemones attack was significantly greater upon exposure to enriched levels of organic sediments and nutrients. Our findings suggest that the combined effects of short-term sedimentation and nutrient enrichment could cause replacement of corals by sea anemones on certain coral reefs. PMID:25897844

  3. Mutualism with sea anemones triggered the adaptive radiation of clownfishes

    PubMed Central

    2012-01-01

    Background Adaptive radiation is the process by which a single ancestral species diversifies into many descendants adapted to exploit a wide range of habitats. The appearance of ecological opportunities, or the colonisation or adaptation to novel ecological resources, has been documented to promote adaptive radiation in many classic examples. Mutualistic interactions allow species to access resources untapped by competitors, but evidence shows that the effect of mutualism on species diversification can greatly vary among mutualistic systems. Here, we test whether the development of obligate mutualism with sea anemones allowed the clownfishes to radiate adaptively across the Indian and western Pacific oceans reef habitats. Results We show that clownfishes morphological characters are linked with ecological niches associated with the sea anemones. This pattern is consistent with the ecological speciation hypothesis. Furthermore, the clownfishes show an increase in the rate of species diversification as well as rate of morphological evolution compared to their closest relatives without anemone mutualistic associations. Conclusions The effect of mutualism on species diversification has only been studied in a limited number of groups. We present a case of adaptive radiation where mutualistic interaction is the likely key innovation, providing new insights into the mechanisms involved in the buildup of biodiversity. Due to a lack of barriers to dispersal, ecological speciation is rare in marine environments. Particular life-history characteristics of clownfishes likely reinforced reproductive isolation between populations, allowing rapid species diversification. PMID:23122007

  4. Nanotoxicology using the sea anemone Nematostella vectensis: from developmental toxicity to genotoxicology.

    PubMed

    Ambrosone, Alfredo; Marchesano, Valentina; Mazzarella, Veronica; Tortiglione, Claudia

    2014-08-01

    Concomitant with the fast-growing advances in the synthesis and engineering of colloidal nanocrystals, an urgent evaluation of their toxicity on human beings and environment is strongly encouraged by public health organisations. Despite the in vitro approaches employed for toxicological screening of hazardous compounds, the use of simple and cost-effective living organisms may enormously contribute to solve unanswered questions related to embryotoxic and teratogenic effects of nanomaterials. Here, the sea anemone Nematostella vectensis (Cnidaria, Anthozoa) is presented as a novel model organism to profile bio/non-bio interactions and to show a comprehensive toxicological analysis performed on embryos, larvae and adults treated with fluorescent cadmium-based nanocrystals. Spanning from in vivo biodistribution to molecular investigations, different behaviours and effects depending on the composition and surface coatings are showed. Rod-shaped cadmium selenide/cadmium sulfide (CdSe/CdS) nanocrystals resulted in excellent imaging probes to track N. vectensis development with negligible adverse effects, while spherical CdTe nanocrystals severely impaired embryogenesis, resulting in aberrant phenotypes and deregulation of developmental genes, which raise severe worries for a safe use of this type of nanoparticles for human purposes and environmental contamination. PMID:23641943

  5. Factors affecting zooplankton feeding by the sea anemone Aiptasia pallida

    NASA Astrophysics Data System (ADS)

    Clayton, William S.

    1986-03-01

    Effects of various treatments on prey capture, prey ingestion and ingestion time of individual Artemia salina nauplii by the sea anemone Aiptasia pallida Verrill were studied in the laboratory. Exposure to crude Artemia homogenate, 5 × 10-4 M reduced glutathione or 5 × 10-4 M proline significantly decreased the number of Artemia that were captured and ingested but had no significant effect on the ingestion time of individual Artemia. Multiple captures increased the total ingestion time but decreased ingestion time per prey item. Results suggest that, under these conditions, the prey capture phase of zooplankton feeding was somewhat distinct from the ingestion phase since chemical stimuli that significantly reduced prey capture had no significant effect on ingestion time.

  6. Expansion of tandem repeats in sea anemone Nematostella vectensis proteome: A source for gene novelty?

    PubMed Central

    2009-01-01

    Background The complete proteome of the starlet sea anemone, Nematostella vectensis, provides insights into gene invention dating back to the Cnidarian-Bilaterian ancestor. With the addition of the complete proteomes of Hydra magnipapillata and Monosiga brevicollis, the investigation of proteins having unique features in early metazoan life has become practical. We focused on the properties and the evolutionary trends of tandem repeat (TR) sequences in Cnidaria proteomes. Results We found that 11-16% of N. vectensis proteins contain tandem repeats. Most TRs cover 150 amino acid segments that are comprised of basic units of 5-20 amino acids. In total, the N. Vectensis proteome has about 3300 unique TR-units, but only a small fraction of them are shared with H. magnipapillata, M. brevicollis, or mammalian proteomes. The overall abundance of these TRs stands out relative to that of 14 proteomes representing the diversity among eukaryotes and within the metazoan world. TR-units are characterized by a unique composition of amino acids, with cysteine and histidine being over-represented. Structurally, most TR-segments are associated with coiled and disordered regions. Interestingly, 80% of the TR-segments can be read in more than one open reading frame. For over 100 of them, translation of the alternative frames would result in long proteins. Most domain families that are characterized as repeats in eukaryotes are found in the TR-proteomes from Nematostella and Hydra. Conclusions While most TR-proteins have originated from prediction tools and are still awaiting experimental validations, supportive evidence exists for hundreds of TR-units in Nematostella. The existence of TR-proteins in early metazoan life may have served as a robust mode for novel genes with previously overlooked structural and functional characteristics. PMID:20003297

  7. Light Entrained Rhythmic Gene Expression in the Sea Anemone Nematostella vectensis: The Evolution of the Animal Circadian Clock

    PubMed Central

    Reitzel, Adam M.; Behrendt, Lars; Tarrant, Ann M.

    2010-01-01

    Background Circadian rhythms in behavior and physiology are the observable phenotypes from cycles in expression of, interactions between, and degradation of the underlying molecular components. In bilaterian animals, the core molecular components include Timeless-Timeout, photoreceptive cryptochromes, and several members of the basic-loop-helix-Per-ARNT-Sim (bHLH-PAS) family. While many of core circadian genes are conserved throughout the Bilateria, their specific roles vary among species. Here, we identify and experimentally study the rhythmic gene expression of conserved circadian clock members in a sea anemone in order to characterize this gene network in a member of the phylum Cnidaria and to infer critical components of the clockwork used in the last common ancestor of cnidarians and bilaterians. Methodology/Principal Findings We identified homologs of circadian regulatory genes in the sea anemone Nematostella vectensis, including a gene most similar to Timeout, three cryptochromes, and several key bHLH-PAS transcription factors. We then maintained N. vectensis either in complete darkness or in a 12 hour light: 12 hour dark cycle in three different light treatments (blue only, full spectrum, blue-depleted). Gene expression varied in response to light cycle and light treatment, with a particularly strong pattern observed for NvClock. The cryptochromes more closely related to the light-sensitive clade of cryptochromes were upregulated in light treatments that included blue wavelengths. With co-immunoprecipitation, we determined that heterodimerization between CLOCK and CYCLE is conserved within N. vectensis. Additionally, we identified E-box motifs, DNA sequences recognized by the CLOCK:CYCLE heterodimer, upstream of genes showing rhythmic expression. Conclusions/Significance This study reveals conserved molecular and functional components of the circadian clock that were in place at the divergence of the Cnidaria and Bilateria, suggesting the animal circadian clockwork is more ancient than previous data suggest. Characterizing circadian regulation in a cnidarian provides insight into the early origins of animal circadian rhythms and molecular regulation of environmentally cued behaviors. PMID:20877728

  8. Population characteristics of the intertidal green sea anemone, Anthopleura xanthogrammica, on the Oregon coast

    NASA Astrophysics Data System (ADS)

    Batchelder, H. P.; Gonor, J. J.

    1981-09-01

    Field population censuses using a photographic quadrat method were used to describe the density and size structure of intertidal populations of the green sea anemone, Anthopleura xanthogrammica, on different types of rocky intertidal habitats on the Oregon coast. Anemone populations in marked plots were monitored for individual motility, growth, mortality and recruitment. Experiments were performed to evaluate anemone population responses to simulated harvesting. Rocky intertidal locations with dense populations of the green anemone are not abundant along the Oregon coast. No detectable fluctuations in anemone population density or size-frequency distribution were found during the 2-year study. Adult anemones showed little movement in either disturbed or undisturbed populations. No natural mortality or recruitment was found in study plots at any Oregon coastal location between July 1977 and May 1979. Removal of adult anemones from rock surfaces did not promote recruitment from the plankton onto the newly available surface, nor did individuals adjacent to depopulated plots rapidly immigrate into the cleared areas. Individual growth is inferred to be very slow in intertidal populations. Field studies indicate that A. xanthogrammica is a long-lived, slow growing species with limited, low or erratic recruitment and highly stable intertidal adult populations. This study indicates that harvest of A. xanthogrammica will probably not be possible on a continuing basis.

  9. A new Fenestrulina (Bryozoa, Cheilostomata) commensal with tube-dwelling anemones (Cnidaria, Ceriantharia) in the tropical southwestern Atlantic.

    PubMed

    Vieira, Leandro M; Stampar, Sergio N

    2014-01-01

    A new species of cheilostome bryozoan, Fenestrulina commensalis n. sp., was collected in December 2008 by scuba at 5-10 meters depth at Guaibura Beach, Guarapari, Espírito Santo state, southeastern Brazil. The specimen was found associated with tubes of the cerianthid Pachycerianthus sp., representing the first commensal association between a bryozoan and a tube-dwelling anemone. Fenestrulina commensalis n. sp. is the third species of the genus found in Brazilian waters; it is distinguished from other Atlantic species of Fenestrulina by its small angular orificial condyles, a single oral spine and basal anchoring rhizoids arising from abfrontal pore chambers. Morphological adaptations to encrust the tubes of cerianthids include anchoring rootlets and weakly contiguous zooids. These morphological features allow the colony the flexibility to grow around the tube and feed relatively undisturbed by silt and detritus, being raised well above the soft-sediment substratum in which the tube-anemone grows. PMID:24871841

  10. Pore formation by actinoporins, cytolysins from sea anemones.

    PubMed

    Rojko, Nejc; Dalla Serra, Mauro; Ma?ek, Peter; Anderluh, Gregor

    2016-03-01

    Actinoporins (APs) from sea anemones are ~20kDa pore forming toxins with a ?-sandwich structure flanked by two ?-helices. The molecular mechanism of APs pore formation is composed of several well-defined steps. APs bind to membrane by interfacial binding site composed of several aromatic amino acid residues that allow binding to phosphatidylcholine and specific recognition of sphingomyelin. Subsequently, the N-terminal ?-helix from the ?-sandwich has to be inserted into the lipid/water interphase in order to form a functional pore. Functional studies and single molecule imaging revealed that only several monomers, 3-4, oligomerise to form a functional pore. In this model the ?-helices and surrounding lipid molecules build toroidal pore. In agreement, AP pores are transient and electrically heterogeneous. On the contrary, crystallized oligomers of actinoporin fragaceatoxin C were found to be composed of eight monomers with no lipids present between the adjacent ?-helices. This article is part of a Special Issue entitled: Pore-Forming Toxins edited by Maur Dalla Serra and Franco Gambale. PMID:26351738

  11. Defending against pathogens – immunological priming and its molecular basis in a sea anemone, cnidarian

    PubMed Central

    Brown, Tanya; Rodriguez-Lanetty, Mauricio

    2015-01-01

    Cnidarians, in general, are long-lived organisms and hence may repeatedly encounter common pathogens during their lifespans. It remains unknown whether these early diverging animals possess some type of immunological reaction that strengthens the defense response upon repeated infections, such as that described in more evolutionary derived organisms. Here we show results that sea anemones that had previously encountered a pathogen under sub-lethal conditions had a higher survivorship during a subsequently lethal challenge than naïve anemones that encountered the pathogen for the first time. Anemones subjected to the lethal challenge two and four weeks after the sub-lethal exposure presented seven- and five-fold increases in survival, respectively, compared to the naïve anemones. However, anemones challenged six weeks after the sub-lethal exposure showed no increase in survivorship. We argue that this short-lasting priming of the defense response could be ecologically relevant if pathogen encounters are restricted to short seasons characterized by high stress. Furthermore, we discovered significant changes in proteomic profiles between naïve sea anemones and those primed after pathogen exposure suggesting a clear molecular signature associated with immunological priming in cnidarians. Our findings reveal that immunological priming may have evolved much earlier in the tree of life than previously thought. PMID:26628080

  12. Evolution of an ancient venom: recognition of a novel family of cnidarian toxins and the common evolutionary origin of sodium and potassium neurotoxins in sea anemone.

    PubMed

    Jouiaei, Mahdokht; Sunagar, Kartik; Federman Gross, Aya; Scheib, Holger; Alewood, Paul F; Moran, Yehu; Fry, Bryan G

    2015-06-01

    Despite Cnidaria (sea anemones, corals, jellyfish, and hydroids) being the oldest venomous animal lineage, structure-function relationships, phyletic distributions, and the molecular evolutionary regimes of toxins encoded by these intriguing animals are poorly understood. Hence, we have comprehensively elucidated the phylogenetic and molecular evolutionary histories of pharmacologically characterized cnidarian toxin families, including peptide neurotoxins (voltage-gated Na(+) and K(+) channel-targeting toxins: NaTxs and KTxs, respectively), pore-forming toxins (actinoporins, aerolysin-related toxins, and jellyfish toxins), and the newly discovered small cysteine-rich peptides (SCRiPs). We show that despite long evolutionary histories, most cnidarian toxins remain conserved under the strong influence of negative selection-a finding that is in striking contrast to the rapid evolution of toxin families in evolutionarily younger lineages, such as cone snails and advanced snakes. In contrast to the previous suggestions that implicated SCRiPs in the biomineralization process in corals, we demonstrate that they are potent neurotoxins that are likely involved in the envenoming function, and thus represent the first family of neurotoxins from corals. We also demonstrate the common evolutionary origin of type III KTxs and NaTxs in sea anemones. We show that type III KTxs have evolved from NaTxs under the regime of positive selection, and likely represent a unique evolutionary innovation of the Actinioidea lineage. We report a correlation between the accumulation of episodically adaptive sites and the emergence of novel pharmacological activities in this rapidly evolving neurotoxic clade. PMID:25757852

  13. Sea anemone venom as a source of insecticidal peptides acting on voltage-gated Na+ channels

    PubMed Central

    Bosmans, Frank; Tytgat, Jan

    2007-01-01

    Sea anemones produce a myriad of toxic peptides and proteins of which a large group acts on voltage-gated Na+ channels. However, in comparison to other organisms, their venoms and toxins are poorly studied. Most of the known voltage-gated Na+ channel toxins isolated from sea anemone venoms act on neurotoxin receptor site 3 and inhibit the inactivation of these channels. Furthermore, it seems that most of these toxins have a distinct preference for crustaceans. Given the close evolutionary relationship between crustaceans and insects, it is not surprising that sea anemone toxins also profoundly affect insect voltage-gated Na+ channels, which constitutes the scope of this review. For this reason, these peptides can be considered as insecticidal lead compounds in the development of insecticides. PMID:17224168

  14. [Structural Features of Cysteine-Stabilized Polypeptides from Sea Anemones Venoms].

    PubMed

    Mikov, A N; Kozlov, S A

    2015-01-01

    Nowadays, venom-based drug discovery becomes popular again: pharmaceutical companies evaluate animal venom potential as a combinatory library of biologically-active compounds. Collaborations with research groups from academia are intensified, new toxins are being investigated, among which polypeptides are of paramount importance. Sea anemones produce the most diversified, from structural point of view, polypep- tide venom components among other animals. This particular review considers known polypeptide toxins from sea anemones, basically taking into account its classification by primary structural features. The most important functional characteristics are analyzed in each structural class. PMID:26762088

  15. Cadherin-23 May Be Dynamic in Hair Bundles of the Model Sea Anemone Nematostella vectensis

    PubMed Central

    Tang, Pei-Ciao; Watson, Glen M.

    2014-01-01

    Cadherin 23 (CDH23), a component of tip links in hair cells of vertebrate animals, is essential to mechanotransduction by hair cells in the inner ear. A homolog of CDH23 occurs in hair bundles of sea anemones. Anemone hair bundles are located on the tentacles where they detect the swimming movements of nearby prey. The anemone CDH23 is predicted to be a large polypeptide featuring a short exoplasmic C-terminal domain that is unique to sea anemones. Experimentally masking this domain with antibodies or mimicking this domain with free peptide rapidly disrupts mechanotransduction and morphology of anemone hair bundles. The loss of normal morphology is accompanied, or followed by a decrease in F-actin in stereocilia of the hair bundles. These effects were observed at very low concentrations of the reagents, 0.1–10 nM, and within minutes of exposure. The results presented herein suggest that: (1) the interaction between CDH23 and molecular partners on stereocilia of hair bundles is dynamic and; (2) the interaction is crucial for normal mechanotransduction and morphology of hair bundles. PMID:24465885

  16. The Involvement of Arl-5b in the Repair of Hair Cells in Sea Anemones

    PubMed Central

    Graugnard, Erin M.; Mire, Patricia

    2007-01-01

    The subcellular processes involved in repair of hair cells are not well understood. Sea anemones repair hair bundle mechanoreceptors on their tentacles after severe trauma caused by 1-h exposure to calcium-depleted seawater. Repair is dependent on the synthesis and secretion of large protein complexes named “repair proteins.” A cDNA library on traumatized anemone tissue was probed using polyclonal antibodies raised to a specific chromatographic fraction of the repair protein mixture. An ADP-ribosylation factor-like protein, Arl-5b, was identified. The amino acid sequence of the Arl-5b protein in sea anemones is similar to that among several model vertebrates and humans. A polyclonal antibody raised to a peptide of the anemone Arl-5b labels some but not all hair bundles in healthy control animals. The abundance of labeled hair bundles significantly increases above healthy controls after trauma and continuing through the first hour of recovery. Dilute anti-Arl-5b blocks the spontaneous repair of hair bundle mechanoreceptors, suggesting that Arl-5b acts on the extracellular face of the plasma membrane. Immunoelectron microscopy indicates that Arl-5b is located along the length of stereocilia including sites in the vicinity of tip links. We propose that Arl-5b is involved in installing replacement linkages into damaged hair bundle mechanoreceptors. PMID:17332968

  17. Seasonal gametogenesis of host sea anemone ( Entacmaea quadricolor) inhabiting Hong Kong waters

    NASA Astrophysics Data System (ADS)

    Bi, Ying; Zhang, Bin; Zhang, Zhifeng; Qiu, Jianwen

    2015-02-01

    Studying gonadal development of annual cycle can reveal the process of gametogenesis and reproductive period, and evaluate fertility and source utilization of a species. Host sea anemones are conspicuous members of tropical and subtropical reef ecosystems, but little is known about its biology including reproductive seasonality. Here we reported a one-year study on the gametogenesis and reproduction of host sea anemone ( Entacmaea quadricolor) inhabiting Hong Kong waters. E. quadricolor tissues were sampled in 12 occasions from 5 m and 15 m depths of water, respectively. Histological sectioning of the tissues showed that E. quadricolor was dioecious, and populational ratio of female to male was 1:1.6. The gonadal development was asynchronous within an annual cycle, which included proliferating, growing, maturing, spawning, and resting stages. The spawning occurred between August and October when surface seawater temperature reached the annual maximum (28°C), suggesting that temperature is an important factor modulating the gonadal development and mature of E. quadricolor.

  18. GABA and glutamate immunoreactivity in tentacles of the sea anemone Phymactis papillosa (LESSON 1830).

    PubMed

    Delgado, Luz M; Couve, Eduardo; Schmachtenberg, Oliver

    2010-07-01

    Sea anemones have a structurally simple nervous system that controls behaviors like feeding, locomotion, aggression, and defense. Specific chemical and tactile stimuli are transduced by ectodermal sensory cells and transmitted via a neural network to cnidocytes and epithelio-muscular cells, but the nature of the neurotransmitters operating in these processes is still under discussion. Previous studies demonstrated an important role of peptidergic transmission in cnidarians, but during the last decade the contribution of conventional neurotransmitters became increasingly evident. Here, we used immunohistochemistry on light and electron microscopical preparations to investigate the localization of glutamate and GABA in tentacle cross-sections of the sea anemone Phymactis papillosa. Our results demonstrate strong glutamate immunoreactivity in the nerve plexus, while GABA labeling was most prominent in the underlying epithelio-muscular layer. Immunoreactivity for both molecules was also found in glandular epithelial cells, and putative sensory cells were GABA positive. Under electron microscopy, both glutamate and GABA immunogold labeling was found in putative neural processes within the neural plexus. These data support a function of glutamate and GABA as signaling molecules in the nervous system of sea anemones. PMID:20309875

  19. Another bipolar deep-sea anemone: new species of Iosactis (Actiniaria, Endomyaria) from Antarctica

    NASA Astrophysics Data System (ADS)

    Rodríguez, Estefanía

    2012-06-01

    A new species of deep-sea burrowing sea anemone is described and illustrated from Antarctica. Iosactis antarctica sp. nov. is characterised by easily deciduous tentacles with sphincters in the base, smooth column, endodermal marginal sphincter, same mesenteries proximally and distally, 24 perfect mesenteries regularly arranged, diffuse retractor musculature and basilar muscles well developed. Iosactis antarctica sp. nov. is the second species of the deep-sea abyssal genus Iosactis; it differs from I. vagabunda in internal anatomy, cnidae and geographic distribution. The description of I. antarctica sp. nov. provides the opportunity to revaluate the morphology of the proximal end of this genus.

  20. Tiny Sea Anemone from the Lower Cambrian of China

    PubMed Central

    Han, Jian; Kubota, Shin; Uchida, Hiro-omi; Stanley, George D.; Yao, Xiaoyong; Shu, Degan; Li, Yong; Yasui, Kinya

    2010-01-01

    Background Abundant fossils from the Ediacaran and Cambrian showing cnidarian grade grossly suggest that cnidarian diversification occurred earlier than that of other eumetazoans. However, fossils of possible soft-bodied polyps are scanty and modern corals are dated back only to the Middle Triassic, although molecular phylogenetic results support the idea that anthozoans represent the first major branch of the Cnidaria. Because of difficulties in taxonomic assignments owing to imperfect preservation of fossil cnidarian candidates, little is known about forms ancestral to those of living groups. Methods and Findings We have analyzed the soft-bodied polypoid microfossils Eolympia pediculata gen. et sp. nov. from the lowest Cambrian Kuanchuanpu Formation in southern China by scanning electron microscopy and computer-aided microtomography after isolating fossils from sedimentary rocks by acetic acid maceration. The fossils, about a half mm in body size, are preserved with 18 mesenteries including directives bilaterally arranged, 18 tentacles and a stalk-like pedicle. The pedicle suggests a sexual life cycle, while asexual reproduction by transverse fission also is inferred by circumferential grooves on the body column. Conclusions The features found in the present fossils fall within the morphological spectrum of modern Hexacorallia excluding Ceriantharia, and thus Eolympia pediculata could be a stem member for this group. The fossils also demonstrate that basic features characterizing modern hexacorallians such as bilateral symmetry and the reproductive system have deep roots in the Early Cambrian. PMID:20967244

  1. Relationships between host and symbiont cell cycles in sea anemones and their symbiotic dinoflagellates.

    PubMed

    Dimond, James L; Pineda, Rea R; Ramos-Ascherl, Zullaylee; Bingham, Brian L

    2013-10-01

    The processes by which cnidarians and their algal endosymbionts achieve balanced growth and biomass could include coordination of host and symbiont cell cycles. We evaluated this theory with natural populations of sea anemones hosting symbiotic dinoflagellates, focusing on the temperate sea anemone Anthopleura elegantissima symbiotic with Symbiodinium muscatinei in Washington State, USA, and the tropical anemone Stichodactyla helianthus associating with unknown Symbiodinium spp. in Belize. By extruding symbiont-containing gastrodermal cells from the relatively large tentacles of these species and using nuclear staining and flow cytometry, we selectively analyzed cell cycle distributions of the symbionts and the host gastrodermal cells that house them. We found no indications of diel synchrony in host and symbiont G2/M phases, and we observed evidence of diel periodicity only in Symbiodinium spp. associated with S. helianthus but not in the anemone itself. Seasonally, S. muscatinei showed considerable G2/M phase variability among samples collected quarterly over an annual period, while the G2/M phase of its host varied much less. Within samples taken at different times of the year, correlations between host and symbiont G2/M phases ranged from very weakly to very strongly positive, with significant correlations in only half of the samples (two of four A. elegantissima samples and one of two S. helianthus samples). Overall, the G2/M phase relationships across species and sampling periods were positive. Thus, while we found no evidence of close cell cycle coupling, our results suggest a loose, positive relationship between cell cycle processes of the symbiotic partners. PMID:24243963

  2. Evidence for participation of GCS1 in fertilization of the starlet sea anemone Nematostella vectensis: Implication of a common mechanism of sperm–egg fusion in plants and animals

    SciTech Connect

    Ebchuqin, Eerdundagula; Yokota, Naoto; Yamada, Lixy; Yasuoka, Yuuri; Akasaka, Mari; Arakawa, Mio; Deguchi, Ryusaku; Mori, Toshiyuki; Sawada, Hitoshi

    2014-09-05

    Highlights: • GCS1 is a sperm transmembrane protein that is essential for gamete fusion in flowering plants. • The GCS1 gene is present not only in angiosperms but also in unicellular organisms and animals. • NvGCS1 gene is expressed in the testis and GCS1 protein exists in sperm of a sea anemone. • Anti-GCS1 antibodies inhibited the fertilization, showing the participation in fertilization. - Abstract: It has been reported that GCS1 (Generative Cell Specific 1) is a transmembrane protein that is exclusively expressed in sperm cells and is essential for gamete fusion in flowering plants. The GCS1 gene is present not only in angiosperms but also in unicellular organisms and animals, implying the occurrence of a common or ancestral mechanism of GCS1-mediated gamete fusion. In order to elucidate the common mechanism, we investigated the role of GCS1 in animal fertilization using a sea anemone (Cnidaria), Nematostella vectensis. Although the existence of the GCS1 gene in N. vectensis has been reported, the expression of GCS1 in sperm and the role of GCS1 in fertilization are not known. In this study, we showed that the GCS1 gene is expressed in the testis and that GCS1 protein exists in sperm by in situ hybridization and proteomic analysis, respectively. Then we made four peptide antibodies against the N-terminal extracellular region of NvGCS1. These antibodies specifically reacted to NvGCS1 among sperm proteins on the basis of Western analysis and potently inhibited fertilization in a concentration-dependent manner. These results indicate that sperm GCS1 plays a pivotal role in fertilization, most probably in sperm–egg fusion, in a starlet sea anemone, suggesting a common gamete-fusion mechanism shared by eukaryotic organisms.

  3. Influence of possible in situ ionic environment on kinetics of purified citrate synthase from an osmoconformer sea anemone, Bunedosoma cavernata.

    PubMed

    Sarkissian, I V; Boatwright, D T

    1975-01-01

    Kinetics of utilization of acetyl coenzyme A by citrate synthase of a sea anemone, an osmoconformer, were compared with those of citrate synthases of various osmoregulators. The Kms of the latter enzymes were substantially increased by higher concentrations of salt and the enzyme exhibited hyperbolic substrate saturation curves. Citrate synthase from sea anemone, on the other hand, exhibited allosteric kinetics and minimal effects of salt on its Km. We suggest that the adaptive advantage of this enzymic property to a sedentary osmoconforming organism such as sea anemone is obvious since the osmoregulating creatures are apparently unable to maintain an appropriate low ionic environment in situ and thus probably the Km of their citrate synthases at a low level. PMID:236904

  4. Short Toxin-like Proteins Abound in Cnidaria Genomes

    PubMed Central

    Tirosh, Yitshak; Linial, Itai; Askenazi, Manor; Linial, Michal

    2012-01-01

    Cnidaria is a rich phylum that includes thousands of marine species. In this study, we focused on Anthozoa and Hydrozoa that are represented by the Nematostella vectensis (Sea anemone) and Hydra magnipapillata genomes. We present a method for ranking the toxin-like candidates from complete proteomes of Cnidaria. Toxin-like functions were revealed using ClanTox, a statistical machine-learning predictor trained on ion channel inhibitors from venomous animals. Fundamental features that were emphasized in training ClanTox include cysteines and their spacing along the sequences. Among the 83,000 proteins derived from Cnidaria representatives, we found 170 candidates that fulfill the properties of toxin-like-proteins, the vast majority of which were previously unrecognized as toxins. An additional 394 short proteins exhibit characteristics of toxin-like proteins at a moderate degree of confidence. Remarkably, only 11% of the predicted toxin-like proteins were previously classified as toxins. Based on our prediction methodology and manual annotation, we inferred functions for over 400 of these proteins. Such functions include protease inhibitors, membrane pore formation, ion channel blockers and metal binding proteins. Many of the proteins belong to small families of paralogs. We conclude that the evolutionary expansion of toxin-like proteins in Cnidaria contributes to their fitness in the complex environment of the aquatic ecosystem. PMID:23202321

  5. Short toxin-like proteins abound in Cnidaria genomes.

    PubMed

    Tirosh, Yitshak; Linial, Itai; Askenazi, Manor; Linial, Michal

    2012-11-01

    Cnidaria is a rich phylum that includes thousands of marine species. In this study, we focused on Anthozoa and Hydrozoa that are represented by the Nematostella vectensis (Sea anemone) and Hydra magnipapillata genomes. We present a method for ranking the toxin-like candidates from complete proteomes of Cnidaria. Toxin-like functions were revealed using ClanTox, a statistical machine-learning predictor trained on ion channel inhibitors from venomous animals. Fundamental features that were emphasized in training ClanTox include cysteines and their spacing along the sequences. Among the 83,000 proteins derived from Cnidaria representatives, we found 170 candidates that fulfill the properties of toxin-like-proteins, the vast majority of which were previously unrecognized as toxins. An additional 394 short proteins exhibit characteristics of toxin-like proteins at a moderate degree of confidence. Remarkably, only 11% of the predicted toxin-like proteins were previously classified as toxins. Based on our prediction methodology and manual annotation, we inferred functions for over 400 of these proteins. Such functions include protease inhibitors, membrane pore formation, ion channel blockers and metal binding proteins. Many of the proteins belong to small families of paralogs. We conclude that the evolutionary expansion of toxin-like proteins in Cnidaria contributes to their fitness in the complex environment of the aquatic ecosystem. PMID:23202321

  6. Responses of the sea anemone, Exaiptasia pallida, to ocean acidification conditions and copper exposure.

    PubMed

    Siddiqui, Samreen; Bielmyer-Fraser, Gretchen K

    2015-10-01

    Ocean acidification (OA) is a growing concern due to its deleterious effects on aquatic organisms. Additionally, the combined effects of OA and other local stressors like metal pollution are largely unknown. In this study, we examined physiological effects in the sea anemone, Exaiptasia pallida after exposure to the global stressor carbon dioxide (CO2), as well as the local stressor copper (Cu) over 7 days. Cu accumulated in the tissues of E. pallida in a concentration-dependent manner. At some time points, sea anemones exposed to 1000 ppm CO2 had higher tissue Cu concentrations than those exposed to 400 ppm CO2 at the same Cu exposure concentrations. In general, the activities of all anti-oxidant enzymes measured (catalase, CAT; glutathione peroxidase, GPx, glutathione reductase, GR) increased with exposure to increasing Cu concentrations. Significant differences in GR, CAT and to some degree GPx activity, were observed due to increasing CO2 exposure in control treatments. Sea anemones exposed to Cu in combination with higher CO2 generally had higher anti-oxidant enzyme activities than those exposed to the same concentration of Cu and lower CO2. Activity of the enzyme, carbonic anhydrase (CA), involved in acid-base balance, was significantly decreased with increasing Cu exposure. At the two lowest Cu concentrations, the extent of CA inhibition was lessened with increasing CO2 concentration. These results provide insight into toxic mechanisms of both Cu and CO2 exposure to the sensitive cnidarian E. pallida and have implications for environmental exposure of multiple contaminants. PMID:26363274

  7. Severe Toxic Skin Reaction Caused by a Common Anemone and Identification of the Culprit Organism.

    PubMed

    Tezcan, Özgür Deniz; Gözer, Özgür

    2015-01-01

    In a marine envenomation, identification of the culprit organism can be difficult. In this case report, we present our method to identify snakelocks anemone (Anemonia viridis or formerly Anemonia sulcata) as the culprit of a severe toxic skin reaction. A. viridis is one of the most common anemones of the Mediterranean Sea and the North Atlantic Ocean. It lives at a depth of up to 10 m. It is a member of the phylum Cnidaria, which includes jellyfish, anemones, hydroids, and corals. They have toxic organelles called cnidocysts that have the capacity to inject venom with microscopic harpoon-like structures. The cnidocysts of A. viridis may cause toxic and allergic reactions, and although its venom is one of the most studied cnidarian venoms, detailed case reports are rare. PMID:26146820

  8. The Global Diversity of Sea Pens (Cnidaria: Octocorallia: Pennatulacea)

    PubMed Central

    Williams, Gary C.

    2011-01-01

    Recent advances in deep-sea exploration technology coupled with an increase in worldwide biotic surveys, biological research, and underwater photography in shallow water marine regions such as coral reefs, has allowed for a relatively rapid expansion of our knowledge in the global diversity of many groups of marine organisms. This paper is part of the PLoS ONE review collection of WoRMS (the Worldwide Register of Marine Species), on the global diversity of marine species, and treats the pennatulacean octocorals, a group of cnidarians commonly referred to as sea pens or sea feathers. This also includes sea pansies, some sea whips, and various vermiform taxa. Pennatulaceans are a morphologically diverse group with an estimated 200 or more valid species, displaying worldwide geographic and bathymetric distributions from polar seas to the equatorial tropics and from intertidal flats to over 6100 m in depth. The paper treats new discoveries and taxa new to science, and provides greater resolution in geographic and bathymetric distributions data than was previously known, as well as descriptions of life appearances in life and in situ observations at diverse depth. PMID:21829500

  9. Indole Alkaloids from the Sea Anemone Heteractis aurora and Homarine from Octopus cyanea.

    PubMed

    Shaker, Kamel H; Göhl, Matthias; Müller, Tobias; Seifert, Karlheinz

    2015-11-01

    The two new indole alkaloids 2-amino-1,5-dihydro-5-(1H-indol-3-ylmethyl)-4H-imidazol-4-one (1), 2-amino-5-[(6-bromo-1H-indol-3-yl)methyl]-3,5-dihydro-3-methyl-4H-imidazol-4-one (2), and auramine (3) have been isolated from the sea anemone Heteractis aurora. Both indole alkaloids were synthesized for the confirmation of the structures. Homarine (4), along with uracil (5), hypoxanthine (6), and inosine (7) have been obtained from Octopus cyanea. PMID:26567952

  10. A novel fluorescent protein from the deep-sea anemone Cribrinopsis japonica (Anthozoa: Actiniaria).

    PubMed

    Tsutsui, Kenta; Shimada, Eriko; Ogawa, Tomohisa; Tsuruwaka, Yusuke

    2016-01-01

    A fluorescent protein was identified and cloned from the deep-sea anemone Cribrinopsis japonica. Bioluminescence and fluorescence expression were examined by direct observations of live specimens and RNA-Seq analysis. Both approaches revealed a novel green fluorescent protein in the tentacles of the anemone, but bioluminescence was not observed. Behavioural observations revealed that a blue light excited the fluorescence in the tentacles, and initiated a behavioural response whereby the fluorescent tentacles became fully exposed to the blue light. The excitation and emission peaks of C. japonica's fluorescent protein were at 500 and 510 nm, respectively, which were greener than those reported in homologs. Furthermore, this protein was highly tolerant of increased temperatures and repeated freeze-thaw treatments. The current study presents an example of fluorescence in a deep-sea cnidarian, demonstrating that fluorescent proteins could have important roles, regardless of the presence or absence of strong sunlight. It also demonstrates that this deep-sea fluorescent protein has unique characteristics, including high stability, perhaps as an adaptation to the extreme environment. PMID:27002644

  11. A novel fluorescent protein from the deep-sea anemone Cribrinopsis japonica (Anthozoa: Actiniaria)

    PubMed Central

    Tsutsui, Kenta; Shimada, Eriko; Ogawa, Tomohisa; Tsuruwaka, Yusuke

    2016-01-01

    A fluorescent protein was identified and cloned from the deep-sea anemone Cribrinopsis japonica. Bioluminescence and fluorescence expression were examined by direct observations of live specimens and RNA-Seq analysis. Both approaches revealed a novel green fluorescent protein in the tentacles of the anemone, but bioluminescence was not observed. Behavioural observations revealed that a blue light excited the fluorescence in the tentacles, and initiated a behavioural response whereby the fluorescent tentacles became fully exposed to the blue light. The excitation and emission peaks of C. japonica’s fluorescent protein were at 500 and 510 nm, respectively, which were greener than those reported in homologs. Furthermore, this protein was highly tolerant of increased temperatures and repeated freeze–thaw treatments. The current study presents an example of fluorescence in a deep-sea cnidarian, demonstrating that fluorescent proteins could have important roles, regardless of the presence or absence of strong sunlight. It also demonstrates that this deep-sea fluorescent protein has unique characteristics, including high stability, perhaps as an adaptation to the extreme environment. PMID:27002644

  12. Asexual Propagation of Sea Anemones That Host Anemonefishes: Implications for the Marine Ornamental Aquarium Trade and Restocking Programs

    PubMed Central

    Scott, Anna; Hardefeldt, Jannah M.; Hall, Karina C.

    2014-01-01

    Anemonefishes and their host sea anemones form an iconic symbiotic association in reef environments, and are highly sought after in the marine aquarium trade. This study examines asexual propagation as a method for culturing a geographically widespread and commonly traded species of host sea anemone, Entacmaea quadricolor. Two experiments were done: the first to establish whether size or colour morph influenced survival after cutting into halves or quarters; and the second to see whether feeding was needed to maximise survival and growth after cutting. Survival rates were high in both experiments, with 89.3 and 93.8% of the anemones cut in half, and 62.5 and 80.4% cut in quarters surviving in experiments 1 and 2, respectively. Anemones that were cut in half were larger in size, and healed and grew quicker than those cut in quarters. However, even though survival was lower when the individuals were cut in quarters, this treatment produced the greatest number of anemones. Feeding increased oral disc diameter growth and reduced wet weight loss, but did not significantly influence pedal disc diameter. Given that the anemones took up to 56 d to form an off-centre mouth, it is highly likely that feeding may have produced greater effect if the experiment was run for longer. This low technology method of propagation could be used to produce individuals throughout the year and the anemones could then be used to supply the aquarium trade or restock depleted habitats, thus supporting biodiversity conservation in coral reef areas. PMID:25314131

  13. Concerted evolution of sea anemone neurotoxin genes is revealed through analysis of the Nematostella vectensis genome.

    PubMed

    Moran, Yehu; Weinberger, Hagar; Sullivan, James C; Reitzel, Adam M; Finnerty, John R; Gurevitz, Michael

    2008-04-01

    Gene families, which encode toxins, are found in many poisonous animals, yet there is limited understanding of their evolution at the nucleotide level. The release of the genome draft sequence for the sea anemone Nematostella vectensis enabled a comprehensive study of a gene family whose neurotoxin products affect voltage-gated sodium channels. All gene family members are clustered in a highly repetitive approximately 30-kb genomic region and encode a single toxin, Nv1. These genes exhibit extreme conservation at the nucleotide level which cannot be explained by purifying selection. This conservation greatly differs from the toxin gene families of other animals (e.g., snakes, scorpions, and cone snails), whose evolution was driven by diversifying selection, thereby generating a high degree of genetic diversity. The low nucleotide diversity at the Nv1 genes is reminiscent of that reported for DNA encoding ribosomal RNA (rDNA) and 2 hsp70 genes from Drosophila, which have evolved via concerted evolution. This evolutionary pattern was experimentally demonstrated in yeast rDNA and was shown to involve unequal crossing-over. Through sequence analysis of toxin genes from multiple N. vectensis populations and 2 other anemone species, Anemonia viridis and Actinia equina, we observed that the toxin genes for each sea anemone species are more similar to one another than to those of other species, suggesting they evolved by manner of concerted evolution. Furthermore, in 2 of the species (A. viridis and A. equina) we found genes that evolved under diversifying selection, suggesting that concerted evolution and accelerated evolution may occur simultaneously. PMID:18222944

  14. Abundance and diversity of anemonefishes and their host sea anemones at two mesophotic sites on the Great Barrier Reef, Australia

    NASA Astrophysics Data System (ADS)

    Bridge, T.; Scott, A.; Steinberg, D.

    2012-12-01

    Anemonefishes and their host sea anemones are iconic inhabitants of coral reef ecosystems. While studies have documented their abundance in shallow-water reef habitats in parts of the Indo-Pacific, none have examined these species on mesophotic reefs. In this study, we used autonomous underwater vehicle imagery to examine the abundance and diversity of anemones and anemonefishes at Viper Reef and Hydrographers Passage in the central Great Barrier Reef at depths between 50 and 65 m. A total of 37 host sea anemones (31 Entacmaea quadricolor and 6 Heteractis crispa) and 24 anemonefishes (23 Amphiprion akindynos and 1 A. perideraion) were observed. Densities were highest at Viper Reef, with 8.48 E. quadricolor and A. akindynos per 100 m2 of reef substratum. These results support the hypothesis that mesophotic reefs have many species common to shallow-water coral reefs and that many taxa may occur at depths greater than currently recognised.

  15. New Kunitz-Type HCRG Polypeptides from the Sea Anemone Heteractis crispa.

    PubMed

    Gladkikh, Irina; Monastyrnaya, Margarita; Zelepuga, Elena; Sintsova, Oksana; Tabakmakher, Valentin; Gnedenko, Oksana; Ivanov, Alexis; Hua, Kuo-Feng; Kozlovskaya, Emma

    2015-10-01

    Sea anemones are a rich source of Kunitz-type polypeptides that possess not only protease inhibitor activity, but also Kv channels toxicity, analgesic, antihistamine, and anti-inflammatory activities. Two Kunitz-type inhibitors belonging to a new Heteractis crispa RG (HCRG) polypeptide subfamily have been isolated from the sea anemone Heteractis crispa. The amino acid sequences of HCRG1 and HCRG2 identified using the Edman degradation method share up to 95% of their identity with the representatives of the HCGS polypeptide multigene subfamily derived from H. crispa cDNA. Polypeptides are characterized by positively charged Arg at the N-terminus as well as P1 Lys residue at their canonical binding loop, identical to those of bovine pancreatic trypsin inhibitor (BPTI). These polypeptides are shown by our current evidence to be more potent inhibitors of trypsin than the known representatives of the HCGS subfamily with P1Thr. The kinetic and thermodynamic characteristics of the intermolecular interactions between inhibitors and serine proteases were determined by the surface plasmon resonance (SPR) method. Residues functionally important for polypeptide binding to trypsin were revealed using molecular modeling methods. Furthermore, HCRG1 and HCRG2 possess anti-inflammatory activity, reducing tumor necrosis factor-? (TNF-?) and interleukin 6 (IL-6) secretions, as well as proIL-1? expression in lipopolysaccharide (LPS)-activated macrophages. However, there was no effect on nitric oxide (NO) generation. PMID:26404319

  16. New Kunitz-Type HCRG Polypeptides from the Sea Anemone Heteractis crispa

    PubMed Central

    Gladkikh, Irina; Monastyrnaya, Margarita; Zelepuga, Elena; Sintsova, Oksana; Tabakmakher, Valentin; Gnedenko, Oksana; Ivanov, Alexis; Hua, Kuo-Feng; Kozlovskaya, Emma

    2015-01-01

    Sea anemones are a rich source of Kunitz-type polypeptides that possess not only protease inhibitor activity, but also Kv channels toxicity, analgesic, antihistamine, and anti-inflammatory activities. Two Kunitz-type inhibitors belonging to a new Heteractis crispa RG (HCRG) polypeptide subfamily have been isolated from the sea anemone Heteractis crispa. The amino acid sequences of HCRG1 and HCRG2 identified using the Edman degradation method share up to 95% of their identity with the representatives of the HCGS polypeptide multigene subfamily derived from H. crispa cDNA. Polypeptides are characterized by positively charged Arg at the N-terminus as well as P1 Lys residue at their canonical binding loop, identical to those of bovine pancreatic trypsin inhibitor (BPTI). These polypeptides are shown by our current evidence to be more potent inhibitors of trypsin than the known representatives of the HCGS subfamily with P1Thr. The kinetic and thermodynamic characteristics of the intermolecular interactions between inhibitors and serine proteases were determined by the surface plasmon resonance (SPR) method. Residues functionally important for polypeptide binding to trypsin were revealed using molecular modeling methods. Furthermore, HCRG1 and HCRG2 possess anti-inflammatory activity, reducing tumor necrosis factor-? (TNF-?) and interleukin 6 (IL-6) secretions, as well as proIL-1? expression in lipopolysaccharide (LPS)-activated macrophages. However, there was no effect on nitric oxide (NO) generation. PMID:26404319

  17. Molecular characterization of two CuZn-superoxide dismutases in a sea anemone.

    PubMed

    Plantivaux, Amandine; Furla, Paola; Zoccola, Didier; Garello, Ginette; Forcioli, Didier; Richier, Sophie; Merle, Pierre-Laurent; Tambutté, Eric; Tambutté, Sylvie; Allemand, Denis

    2004-10-15

    Cnidarians living in symbiosis with photosynthetic cells--called zooxanthellae--are submitted to high oxygen levels generated by photosynthesis. To cope with this hyperoxic state, symbiotic cnidarians present a high diversity of superoxide dismutases (SOD) isoforms. To understand better the mechanism of resistance of cnidarian hosts to hyperoxia, we studied copper- and zinc-containing SOD (CuZnSOD) from Anemonia viridis, a temperate symbiotic sea anemone. We cloned two CuZnSOD genes that we call AvCuZnSODa and AvCuZnSODb. Their molecular analysis suggests that the AvCuZnSODa transcript encodes an extracellular form of CuZnSOD, whereas the AvCuZnSODb transcript encodes an intracellular form. Using in situ hybridization, we showed that both AvCuZnSODa and AvCuZnSODb transcripts are expressed in the endodermal and ectodermal cells of the sea anemone, but not in the zooxanthellae. The genomic flanking sequences of AvCuZnSODa and AvCuZnSODb revealed different putative binding sites for transcription factors, suggesting different modes of regulation for the two genes. This study represents a first step in the understanding of the molecular mechanisms of host animal resistance to permanent hyperoxia status resulting from the photosynthetic symbiosis. Moreover, AvCuZnSODa and AvCuZnSODb are the first SODs cloned from a diploblastic animal, contributing to the evolutionary understanding of SODs. PMID:15451057

  18. Fusion and retrotransposition events in the evolution of the sea anemone Anemonia viridis neurotoxin genes.

    PubMed

    Moran, Yehu; Weinberger, Hagar; Lazarus, Nimrod; Gur, Maya; Kahn, Roy; Gordon, Dalia; Gurevitz, Michael

    2009-08-01

    Sea anemones are sessile predators that use a variety of toxins to paralyze prey and foe. Among these toxins, Types I, II and III are short peptides that affect voltage-gated sodium channels. Anemonia viridis is the only sea anemone species that produces both Types I and III neurotoxin. Although the two toxin types are unrelated in sequence and three-dimensional structure, cloning and comparative analysis of their loci revealed a highly similar sequence at the 5' region, which encodes a signal peptide. This similarity was likely generated by gene fusion and could be advantageous in transcript stability and intracellular trafficking and secretion. In addition, these analyses identified the processed pseudogenes of the two gene families in the genome of A. viridis, probably resulting from retrotransposition events. As presence of processed pseudogenes in the genome requires transcription in germ-line cells, we analyzed oocyte-rich ovaries and found that indeed they contain Types I and III transcripts. This result raises questions regarding the role of toxin transcripts in these tissues. Overall, the retrotransposition and gene fusion events suggest that the genes of both Types I and III neurotoxins evolved in a similar fashion and share a partial common ancestry. PMID:19609479

  19. Catalase characterization and implication in bleaching of a symbiotic sea anemone.

    PubMed

    Merle, Pierre-Laurent; Sabourault, Cécile; Richier, Sophie; Allemand, Denis; Furla, Paola

    2007-01-15

    Symbiotic cnidarians are marine invertebrates harboring photosynthesizing microalgae (named zooxanthellae), which produce great amounts of oxygen and free radicals upon illumination. Studying antioxidative balance is then crucial to understanding how symbiotic cnidarians cope with ROS production. In particular, it is suspected that oxidative stress triggers cnidarian bleaching, i.e., the expulsion of zooxanthellae from the animal host, responsible for symbiotic cnidarian mass mortality worldwide. This study therefore investigates catalase antioxidant enzymes and their role in bleaching of the temperate symbiotic sea anemone Anemonia viridis. Using specific separation of animal tissues (ectoderm and endoderm) from the symbionts (zooxanthellae), spectrophotometric assays and native PAGE revealed both tissue-specific and activity pattern distribution of two catalase electrophoretypes, E1 and E2. E1, expressed in all three tissues, presents high sensitivity to the catalase inhibitor aminotriazole (ATZ) and elevated temperatures. The ectodermal E1 form is responsible for 67% of total catalase activity. The E2 form, expressed only within zooxanthellae and their host endodermal cells, displays low sensitivity to ATZ and relative thermostability. We further cloned an ectodermal catalase, which shares 68% identity with mammalian monofunctional catalases. Last, 6 days of exposure of whole sea anemones to ATZ (0.5 mM) led to effective catalase inhibition and initiated symbiont expulsion. This demonstrates the crucial role of this enzyme in cnidarian bleaching, a phenomenon responsible for worldwide climate-change-induced mass mortalities, with catastrophic consequences for marine biodiversity. PMID:17189829

  20. Bacterial aggregates in the tentacles of the sea anemone Metridium senile

    NASA Astrophysics Data System (ADS)

    Schuett, Christian; Doepke, Hilke; Grathoff, Annette; Gedde, Michael

    2007-09-01

    This paper provides first information on organ-like bacterial aggregates in the tentacles of the sea anemone Metridium senile. The specimens were collected from waters near Helgoland (German Bight, North Sea) and the Orkney Islands. Tentacles were prepared for morphological inspection by light and scanning electron microscopy as well as for the phylogenetic analysis of endocytic bacteria. Bacterial aggregates are located in caverns of the tentacles’ epidermis. The aggregates are enwrapped in thin envelopes, which contain coccoid and/or rod-shaped tightly packed bacteria of different division states. Most of the bacterial cells are connected by fine filamentous structures. The phylogenetic determination is based on the sequence data of the 16S rDNA derived from tentacle material. Sequence analysis revealed three different subgroups of intratentacular proteobacteria. The dominant band, detected in all of the samples tested, showed a close relationship (98%) to a gram-negative Endozoicimonas elysicola. Two bands, only detected in tentacles of M. senile from Helgoland were assigned to Pseudomonas saccherophilia (99%), a knallgas bacterium, and to Ralstonia pickettii (100%). The bacteria represent a specific bacterial community. Their DGGE profiles do not correspond to the profiles of the planktonic bacteria generated from seawater close to the habitats of the anemones. The allocation of DNA sequences to the different morphotypes, their isolation, culturing and the elucidation of the physiological functions of intratentacular bacteria are in progress.

  1. Atypical reactive center Kunitz-type inhibitor from the sea anemone Heteractis crispa.

    PubMed

    Gladkikh, Irina; Monastyrnaya, Margarita; Leychenko, Elena; Zelepuga, Elena; Chausova, Victoria; Isaeva, Marina; Anastyuk, Stanislav; Andreev, Yaroslav; Peigneur, Steve; Tytgat, Jan; Kozlovkaya, Emma

    2012-07-01

    The primary structure of a new Kunitz-type protease inhibitor InhVJ from the sea anemone Heteractis crispa (Radianthus macrodactylus) was determined by protein sequencing and cDNA cloning. InhVJ amino acid sequence was shown to share high sequence identity (up to 98%) with the other known Kunitz-type sea anemones sequences. It was determined that the P1 Thr at the reactive site resulted in a decrease of the K(i) of InhVJ to trypsin and ?-chymotrypsin (7.38 × 10(-8) M and 9.93 × 10(-7) M, respectively). By structure modeling the functional importance of amino acids at the reactive site as well as at the weak contact site were determined. The significant role of Glu45 for the orientation and stabilization of the InhVJ-trypsin complex was elucidated. We can suggest that there has been an adaptive evolution of the P1 residue at the inhibitor reactive site providing specialization or functional diversification of the paralogs. The appearance of a key so-called P1 Thr residue instead of Lys might lead to refinement of inhibitor specificity in the direction of subfamilies of serine proteases. The absence of Kv channel and TRPV1-receptor modulation activity was confirmed by electrophysiological screening tests. PMID:22851925

  2. Increased cell proliferation and mucocyte density in the sea anemone Aiptasia pallida recovering from bleaching.

    PubMed

    Fransolet, David; Roberty, Stéphane; Herman, Anne-Catherine; Tonk, Linda; Hoegh-Guldberg, Ove; Plumier, Jean-Christophe

    2013-01-01

    Recovery of coral after bleaching episodes is a critical period for the health of the reef ecosystem. While events such as symbiont (genus Symbiodinium) shifting/shuffling or tissue apoptosis have been demonstrated to occur following bleaching, little is known concerning tissue recovery or cell proliferation. Here, we studied the sea anemone Aiptasia pallida exposed to a transient elevation of water temperature combined with high illumination (33°C and 1900 µmol photons x m(-2) x s(-1) for 30 h). Following such treatment bleached anemones showed a significant reduction of their Symbiodinium density. Cell proliferation in the ectodermis and gastrodermis was determined by assessing the densities of cells labeled with a thymidine analogue (EdU). Cell proliferation significantly increased during the first day following stress in both tissue types. This increased cell proliferation returned to pre-stress values after one week. Although cell proliferation was higher in the ectodermis in absence of stress, it was relatively more pronounced in the gastrodermis of stressed anemones. In addition, the ratio of ectodermal mucocytes significantly increased three weeks after induced stress. These results suggest that thermal/photic stress coupled with the loss of the symbionts is able to enhance cell proliferation in both gastrodermis and ectodermis of cnidarians. While new cells formed in the gastrodermis are likely to host new Symbiodinium, the fate of new cells in the ectodermis was only partially revealed. Some new ectodermal cells may, in part, contribute to the increased number of mucocytes which could eventually help strengthen the heterotrophic state until restoration of the symbiosis. PMID:23724115

  3. Trace element profiles of the sea anemone Anemonia viridis living nearby a natural CO2 vent

    PubMed Central

    Borell, Esther M.; Fine, Maoz; Shaked, Yeala

    2014-01-01

    Ocean acidification (OA) is not an isolated threat, but acts in concert with other impacts on ecosystems and species. Coastal marine invertebrates will have to face the synergistic interactions of OA with other global and local stressors. One local factor, common in coastal environments, is trace element contamination. CO2 vent sites are extensively studied in the context of OA and are often considered analogous to the oceans in the next few decades. The CO2 vent found at Levante Bay (Vulcano, NE Sicily, Italy) also releases high concentrations of trace elements to its surrounding seawater, and is therefore a unique site to examine the effects of long-term exposure of nearby organisms to high pCO2 and trace element enrichment in situ. The sea anemone Anemonia viridis is prevalent next to the Vulcano vent and does not show signs of trace element poisoning/stress. The aim of our study was to compare A. viridis trace element profiles and compartmentalization between high pCO2 and control environments. Rather than examining whole anemone tissue, we analyzed two different body compartments—the pedal disc and the tentacles, and also examined the distribution of trace elements in the tentacles between the animal and the symbiotic algae. We found dramatic changes in trace element tissue concentrations between the high pCO2/high trace element and control sites, with strong accumulation of iron, lead, copper and cobalt, but decreased concentrations of cadmium, zinc and arsenic proximate to the vent. The pedal disc contained substantially more trace elements than the anemone’s tentacles, suggesting the pedal disc may serve as a detoxification/storage site for excess trace elements. Within the tentacles, the various trace elements displayed different partitioning patterns between animal tissue and algal symbionts. At both sites iron was found primarily in the algae, whereas cadmium, zinc and arsenic were primarily found in the animal tissue. Our data suggests that A. viridis regulates its internal trace element concentrations by compartmentalization and excretion and that these features contribute to its resilience and potential success at the trace element-rich high pCO2 vent. PMID:25250210

  4. Parazoanthines A-E, hydantoin alkaloids from the Mediterranean sea anemone Parazoanthus axinellae.

    PubMed

    Cachet, Nadja; Genta-Jouve, Grégory; Regalado, Erik L; Mokrini, Redouane; Amade, Philippe; Culioli, Gérald; Thomas, Olivier P

    2009-09-01

    Five new hydantoin alkaloids, named parazoanthines A-E (1-5), were isolated as the major constituents of the Mediterranean sea anemone Parazoanthus axinellae. Their structural elucidation was achieved through NMR spectroscopic and mass spectrometric analyses. The absolute configuration of the chiral compounds 1 and 4 was determined by comparison between experimental and TDDFT-calculated CD spectra. The configuration of the trisubstituted double bond of 2, 3, and 5 was deduced from the (3)J(H6-C4) coupling constant value. This family of alkaloids represents the first example of natural 3,5-disubstituted hydantoins that do not exhibit a methyl at N-3. All compounds were tested for their natural toxicity (Microtox assay), and parazoanthine C (3) exhibited the highest natural toxicity. PMID:19708637

  5. Edwardsiella andrillae, a New Species of Sea Anemone from Antarctic Ice

    PubMed Central

    Daly, Marymegan; Rack, Frank; Zook, Robert

    2013-01-01

    Exploration of the lower surface of the Ross Ice Shelf in Antarctica by the Submersible Capable of under-Ice Navigation and Imaging (SCINI) remotely operated vehicle discovered a new species of sea anemone living in this previously undocumented ecosystem. This discovery was a significant outcome of the Coulman High Project’s geophysical and environmental fieldwork in 2010-2011 as part of the ANDRILL (ANtarctic geologic DRILLing) program. Edwardsiella andrillae n. sp., lives with most of its column in the ice shelf, with only the tentacle crown extending into the seawater below. In addition to being the only Antarctic representative of the genus, Edwardsiella andrillae is distinguished from all other species of the genus in the number of tentacles and in the size and distribution of cnidae. The anatomy and histology of Edwardsiella andrillae present no features that explain how this animal withstands the challenges of life in such an unusual habitat. PMID:24349517

  6. Trace element profiles of the sea anemone Anemonia viridis living nearby a natural CO2 vent.

    PubMed

    Horwitz, Rael; Borell, Esther M; Fine, Maoz; Shaked, Yeala

    2014-01-01

    Ocean acidification (OA) is not an isolated threat, but acts in concert with other impacts on ecosystems and species. Coastal marine invertebrates will have to face the synergistic interactions of OA with other global and local stressors. One local factor, common in coastal environments, is trace element contamination. CO2 vent sites are extensively studied in the context of OA and are often considered analogous to the oceans in the next few decades. The CO2 vent found at Levante Bay (Vulcano, NE Sicily, Italy) also releases high concentrations of trace elements to its surrounding seawater, and is therefore a unique site to examine the effects of long-term exposure of nearby organisms to high pCO2 and trace element enrichment in situ. The sea anemone Anemonia viridis is prevalent next to the Vulcano vent and does not show signs of trace element poisoning/stress. The aim of our study was to compare A. viridis trace element profiles and compartmentalization between high pCO2 and control environments. Rather than examining whole anemone tissue, we analyzed two different body compartments-the pedal disc and the tentacles, and also examined the distribution of trace elements in the tentacles between the animal and the symbiotic algae. We found dramatic changes in trace element tissue concentrations between the high pCO2/high trace element and control sites, with strong accumulation of iron, lead, copper and cobalt, but decreased concentrations of cadmium, zinc and arsenic proximate to the vent. The pedal disc contained substantially more trace elements than the anemone's tentacles, suggesting the pedal disc may serve as a detoxification/storage site for excess trace elements. Within the tentacles, the various trace elements displayed different partitioning patterns between animal tissue and algal symbionts. At both sites iron was found primarily in the algae, whereas cadmium, zinc and arsenic were primarily found in the animal tissue. Our data suggests that A. viridis regulates its internal trace element concentrations by compartmentalization and excretion and that these features contribute to its resilience and potential success at the trace element-rich high pCO2 vent. PMID:25250210

  7. Analgesic Compound from Sea Anemone Heteractis crispa Is the First Polypeptide Inhibitor of Vanilloid Receptor 1 (TRPV1)*

    PubMed Central

    Andreev, Yaroslav A.; Kozlov, Sergey A.; Koshelev, Sergey G.; Ivanova, Ekaterina A.; Monastyrnaya, Margarita M.; Kozlovskaya, Emma P.; Grishin, Eugene V.

    2008-01-01

    Venomous animals from distinct phyla such as spiders, scorpions, snakes, cone snails, or sea anemones produce small toxic proteins interacting with a variety of cell targets. Their bites often cause pain. One of the ways of pain generation is the activation of TRPV1 channels. Screening of 30 different venoms from spiders and sea anemones for modulation of TRPV1 activity revealed inhibitors in tropical sea anemone Heteractis crispa venom. Several separation steps resulted in isolation of an inhibiting compound. This is a 56-residue-long polypeptide named APHC1 that has a Bos taurus trypsin inhibitor (BPTI)/Kunitz-type fold, mostly represented by serine protease inhibitors and ion channel blockers. APHC1 acted as a partial antagonist of capsaicin-induced currents (32 ± 9% inhibition) with half-maximal effective concentration (EC50) 54 ± 4 nm. In vivo, a 0.1 mg/kg dose of APHC1 significantly prolonged tail-flick latency and reduced capsaicin-induced acute pain. Therefore, our results can make an important contribution to the research into molecular mechanisms of TRPV1 modulation and help to solve the problem of overactivity of this receptor during a number of pathological processes in the organism. PMID:18579526

  8. Reproductive biology of the sea anemone shrimp Periclimenes rathbunae (Caridea, Palaemonidae, Pontoniinae), from the Caribbean coast of Costa Rica

    PubMed Central

    Azofeifa-Solano, Juan Carlos; Elizondo-Coto, Marcelo; Wehrtmann, Ingo S.

    2014-01-01

    Abstract Caridean shrimps are a highly diverse group and many species form symbiotic relationships with different marine invertebrates. Periclimenes rathbunae is a brightly colored shrimp that lives predominantly in association with sea anemones. Information about the reproductive ecology of the species is scarce. Therefore, we collected 70 ovigerous females inhabiting the sun sea anemone Stichodactyla helianthus in coral reefs from the southern Caribbean coast of Costa Rica. Females produced on average 289 ± 120 embryos. The volume of recently-produced embryos was on average 0.038 mm3, and embryo volume increased by 192% during the incubation period. The average embryo mortality during embryogenesis was 24%. The reproductive output was 0.24 ± 0.094, considerably higher than in many other pontoniine shrimps. Females carrying embryos close to hatching showed fully developed ovaries, suggesting consecutive spawning. We assume that the sheltered habitat, living on sea anemones, allows Periclimenes rathbunae to allocate more energy in embryo production than most other free-living caridean shrimps. This is the first record of Periclimenes rathbunae for Costa Rica. PMID:25561838

  9. Oxidative stress and apoptotic events during thermal stress in the symbiotic sea anemone, Anemonia viridis.

    PubMed

    Richier, Sophie; Sabourault, Cécile; Courtiade, Juliette; Zucchini, Nathalie; Allemand, Denis; Furla, Paola

    2006-09-01

    Symbiosis between cnidarian and photosynthetic protists is widely distributed over temperate and tropical seas. These symbioses can periodically breakdown, a phenomenon known as cnidarian bleaching. This event can be irreversible for some associations subjected to acute and/or prolonged environmental disturbances, and leads to the death of the animal host. During bleaching, oxidative stress has been described previously as acting at molecular level and apoptosis is suggested to be one of the mechanisms involved. We focused our study on the role of apoptosis in bleaching via oxidative stress in the association between the sea anemone Anemonia viridis and the dinoflagellates Symbiodinium species. Characterization of caspase-like enzymes were conducted at the biochemical and molecular level to confirm the presence of a caspase-dependent apoptotic phenomenon in the cnidarian host. We provide evidence of oxidative stress followed by induction of caspase-like activity in animal host cells after an elevated temperature stress, suggesting the concomitant action of these components in bleaching. PMID:16907933

  10. Mechanically Durable and Biologically Favorable Protein Hydrogel Based on Elastic Silklike Protein Derived from Sea Anemone.

    PubMed

    Yang, Yun Jung; Kim, Chang Sup; Choi, Bong-Hyuk; Cha, Hyung Joon

    2015-12-14

    As biodegradable scaffolds, protein hydrogels have considerable potential, particularly for bioartificial organs and three-dimensional space-filling materials. However, their low strength and stiffness have been considered to be limitations for enduring physiological stimuli. Therefore, protein hydrogels have been commonly utilized as delivery vehicles rather than as supporting materials. In this work, sea anemone tentacle-derived recombinant silk-like protein (aneroin) was evaluated as a potential material for a mechanically durable protein hydrogel. Inspired by the natural hardening mechanism, photoinitiated dityrosine cross-linking was employed to fabricate an aneroin hydrogel. It was determined that the fabricated aneroin hydrogel was approximately 10-fold stiffer than mammalian cardiac or skeletal muscle. The aneroin hydrogel provided not only structural support but also an adequate environment for cells. It exhibited an adequate swelling ability and microstructure, which are beneficial for facilitating mass transport and cell proliferation. Based on its mechanical and biological properties, this aneroin hydrogel could be used in various biomedical applications, such as cell-containing patches, biomolecule carriers, and artificial extracellular matrices. PMID:26539814

  11. Profiling molecular and behavioral circadian rhythms in the non-symbiotic sea anemone Nematostella vectensis

    PubMed Central

    Oren, Matan; Tarrant, Ann M.; Alon, Shahar; Simon-Blecher, Noa; Elbaz, Idan; Appelbaum, Lior; Levy, Oren

    2015-01-01

    Endogenous circadian clocks are poorly understood within early-diverging animal lineages. We have characterized circadian behavioral patterns and identified potential components of the circadian clock in the starlet sea anemone, Nematostella vectensis: a model cnidarian which lacks algal symbionts. Using automatic video tracking we showed that Nematostella exhibits rhythmic circadian locomotor activity, which is persistent in constant dark, shifted or disrupted by external dark/light cues and maintained the same rate at two different temperatures. This activity was inhibited by a casein kinase 1δ/ε inhibitor, suggesting a role for CK1 homologue(s) in Nematostella clock. Using high-throughput sequencing we profiled Nematostella transcriptomes over 48 hours under a light-dark cycle. We identified 180 Nematostella diurnally-oscillated transcripts and compared them with previously established databases of adult and larvae of the symbiotic coral Acropora millepora, revealing both shared homologues and unique rhythmic genes. Taken together, this study further establishes Nematostella as a non-symbiotic model organism to study circadian rhythms and increases our understanding about the fundamental elements of circadian regulation and their evolution within the Metazoa PMID:26081482

  12. Sexual Plasticity and Self-Fertilization in the Sea Anemone Aiptasia diaphana

    PubMed Central

    Schlesinger, Ami; Kramarsky-Winter, Esti; Rosenfeld, Hanna; Armoza-Zvoloni, Rachel; Loya, Yossi

    2010-01-01

    Traits that influence reproductive success and contribute to reproductive isolation in animal and plant populations are a central focus of evolutionary biology. In the present study we used an experimental approach to demonstrate the occurrence of environmental effects on sexual and asexual reproduction, and provide evidence for sexual plasticity and inter-clonal fertilization in laboratory-cultured lines of the sea anemone Aiptasia diaphana. We showed that in A. diaphana, both asexual reproduction by pedal laceration, and sexual reproduction have seasonal components. The rate of pedal laceration was ten-fold higher under summer photoperiod and water temperature conditions than under winter conditions. The onset of gametogenesis coincided with the rising water temperatures occurring in spring, and spawning occurred under parameters that emulated summer photoperiod and temperature conditions. In addition, we showed that under laboratory conditions, asexually produced clones derived from a single founder individual exhibit sexual plasticity, resulting in the development of both male and female individuals. Moreover, a single female founder produced not only males and females but also hermaphrodite individuals. We further demonstrated that A. diaphana can fertilize within and between clone lines, producing swimming planula larvae. These diverse reproductive strategies may explain the species success as invader of artificial marine substrates. We suggest that these diverse reproductive strategies, together with their unique evolutionary position, make Aiptasia diaphana an excellent model for studying the evolution of sex. PMID:20686700

  13. The genome of Aiptasia, a sea anemone model for coral symbiosis

    PubMed Central

    Baumgarten, Sebastian; Simakov, Oleg; Esherick, Lisl Y.; Liew, Yi Jin; Lehnert, Erik M.; Michell, Craig T.; Li, Yong; Hambleton, Elizabeth A.; Guse, Annika; Oates, Matt E.; Gough, Julian; Weis, Virginia M.; Aranda, Manuel; Pringle, John R.; Voolstra, Christian R.

    2015-01-01

    The most diverse marine ecosystems, coral reefs, depend upon a functional symbiosis between a cnidarian animal host (the coral) and intracellular photosynthetic dinoflagellate algae. The molecular and cellular mechanisms underlying this endosymbiosis are not well understood, in part because of the difficulties of experimental work with corals. The small sea anemone Aiptasia provides a tractable laboratory model for investigating these mechanisms. Here we report on the assembly and analysis of the Aiptasia genome, which will provide a foundation for future studies and has revealed several features that may be key to understanding the evolution and function of the endosymbiosis. These features include genomic rearrangements and taxonomically restricted genes that may be functionally related to the symbiosis, aspects of host dependence on alga-derived nutrients, a novel and expanded cnidarian-specific family of putative pattern-recognition receptors that might be involved in the animal–algal interactions, and extensive lineage-specific horizontal gene transfer. Extensive integration of genes of prokaryotic origin, including genes for antimicrobial peptides, presumably reflects an intimate association of the animal–algal pair also with its prokaryotic microbiome. PMID:26324906

  14. Mercury distribution, methylation and volatilization in microcosms with and without the sea anemone Bunodosoma caissarum.

    PubMed

    Rizzini Ansari, Nafisa; Correia, Raquel Rose Silva; Fernandez, Marcos Antônio; Cordeiro, Renato Campello; Guimarães, Jean Remy Davée

    2015-03-15

    Mercury (Hg) has a complex biogeochemical cycle in aquatic environments. Its most toxic form, methylmercury (MeHg), is produced by microorganisms. This study investigated how the sea anemone Bunodosoma caissarum affects Hg distribution, methylation and volatilization in laboratory model systems. (203)Hg was added to microcosms and its distribution in seawater, specimens and air was periodically measured by gamma spectrometry. MeHg was measured by liquid scintillation. After the uptake period, specimens had a bioconcentration factor of 70 and in microcosms with and without B. caissarum, respectively 0.05% and 0.32% of the initial spike was found as MeHg. After depuration, MeHg in specimens ranged from 0.2% to 2.4% of total Hg. Microcosms with B. caissarum had higher Hg volatilization (58%) than controls (17%), possibly due to Hg(2+) reduction mediated by microorganisms associated with its tissues and mucus secretions. Marine organisms and their associated microbiota may play a role in Hg and MeHg cycling. PMID:25599628

  15. Unexpected complexity of the Wnt gene family in a sea anemone.

    PubMed

    Kusserow, Arne; Pang, Kevin; Sturm, Carsten; Hrouda, Martina; Lentfer, Jan; Schmidt, Heiko A; Technau, Ulrich; von Haeseler, Arndt; Hobmayer, Bert; Martindale, Mark Q; Holstein, Thomas W

    2005-01-13

    The Wnt gene family encodes secreted signalling molecules that control cell fate in animal development and human diseases. Despite its significance, the evolution of this metazoan-specific protein family is unclear. In vertebrates, twelve Wnt subfamilies were defined, of which only six have counterparts in Ecdysozoa (for example, Drosophila and Caenorhabditis). Here, we report the isolation of twelve Wnt genes from the sea anemone Nematostella vectensis, a species representing the basal group within cnidarians. Cnidarians are diploblastic animals and the sister-group to bilaterian metazoans. Phylogenetic analyses of N. vectensis Wnt genes reveal a thus far unpredicted ancestral diversity within the Wnt family. Cnidarians and bilaterians have at least eleven of the twelve known Wnt gene subfamilies in common; five subfamilies appear to be lost in the protostome lineage. Expression patterns of Wnt genes during N. vectensis embryogenesis indicate distinct roles of Wnts in gastrulation, resulting in serial overlapping expression domains along the primary axis of the planula larva. This unexpectedly complex inventory of Wnt family signalling factors evolved in early multi-cellular animals about 650 million years (Myr) ago, predating the Cambrian explosion by at least 100 Myr (refs 5, 8). It emphasizes the crucial function of Wnt genes in the diversification of eumetazoan body plans. PMID:15650739

  16. [Conformational stability of serine proteinase inhibitor from the sea anemone Heteractis crispa].

    PubMed

    Vakorina, T I; Gladkikh, I N; Monastyrnaia, M M; Kozlovskaia, E P

    2011-01-01

    The influence of different environmental values of the pH and temperature on the spatial organization of serine proteinase inhibitor from the sea anemone Heteractis crispa (=Radianthus macrodactylus) on the level of tertiary and secondary structure was studied by CD spectroscopy. The molecule InhVJ was shown to possess a high conformational thermo- and pH-stability. We determined the point of conformational thermotransition of polypeptide (70 degrees C) after which the molecule gets denaturational stable state with conservation of 80% proteinase inhibitory activity. The significant partial reversible changes of molecule spatial organization were established to occur at the level of tertiary structure in the process of acid-base titration in the range of pH 11.0-13.0. This can be explained by of ionization of tyrosine residues. The molecule InhVJ is conformationally stable at the low pH values (2.0). The quenching of tyrosine residues by acrylamide showed that two of these residues are accessible to the quencher in full, while the third part is available. PMID:21899045

  17. Fast Neurotransmission Related Genes Are Expressed in Non Nervous Endoderm in the Sea Anemone Nematostella vectensis

    PubMed Central

    Oren, Matan; Brikner, Itzchak; Appelbaum, Lior; Levy, Oren

    2014-01-01

    Cnidarian nervous systems utilize chemical transmission to transfer signals through synapses and neurons. To date, ample evidence has been accumulated for the participation of neuropeptides, primarily RFamides, in neurotransmission. Yet, it is still not clear if this is the case for the classical fast neurotransmitters such as GABA, Glutamate, Acetylcholine and Monoamines. A large repertoire of cnidarian Fast Neurotransmitter related Genes (FNGs) has been recently identified in the genome of the sea anemone, Nematostella vectensis. In order to test whether FNGs are localized in cnidarian neurons, we characterized the expression patterns of eight Nematostella genes that are closely or distantly related to human central and peripheral nervous systems genes, in adult Nematostella and compared them to the RFamide localization. Our results show common expression patterns for all tested genes, in a single endodermal cell layer. These expressions did not correspond with the RFamide expressing nerve cell network. Following these results we suggest that the tested Nematostella genes may not be directly involved in vertebrate-like fast neurotransmission. PMID:24705400

  18. Outcomes of infections of sea anemone Aiptasia pallida with Vibrio spp. pathogenic to corals.

    PubMed

    Zaragoza, William J; Krediet, Cory J; Meyer, Julie L; Canas, Gabriela; Ritchie, Kim B; Teplitski, Max

    2014-08-01

    Incidents of coral disease are on the rise. However, in the absence of a surrogate animal host, understanding of the interactions between coral pathogens and their hosts remains relatively limited, compared to other pathosystems of similar global importance. A tropical sea anemone, Aiptasia pallida, has been investigated as a surrogate model to study certain aspects of coral biology. Therefore, to test whether the utility of this surrogate model can be extended to study coral diseases, in the present study, we tested its susceptibility to common coral pathogens (Vibrio coralliilyticus and Vibrio shiloi) as well as polymicrobial consortia recovered from the Caribbean Yellow Band Disease (CYBD) lesions. A. pallida was susceptible to each of the tested pathogens. A. pallida responded to the pathogens with darkening of the tissues (associated with an increased melanization) and retraction of tentacles, followed by complete disintegration of polyp tissues. Loss of zooxanthellae was not observed; however, the disease progression pattern is consistent with the behavior of necrotizing pathogens. Virulence of some coral pathogens in Aiptasia was paralleled with their glycosidase activities. PMID:24619233

  19. Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea

    PubMed Central

    Hu, Bo; Guo, Wei; Wang, Liang-hua; Wang, Jian-guang; Liu, Xiao-yu; Jiao, Bing-hua

    2011-01-01

    A new Cytolysin, termed as Gigantoxin-4, was isolated from the sea anemone Stichodactyla gigantea and found to be highly homologous with Cytolysin-3 (HMg III) from Heteractis magnifica, RTX-A from Radianthus macrodactylus, and Sticholysin-1 (St I) and Sticholysin-2 (St II) from Stichodactyla helianthus (homology 82%, 86%, 82% and 86% respectively). Its 20 N-terminal residues were identified and the full-length cDNA sequence was obtained by reverse transcription-polymerase chain reaction (RT-PCR). Multiple sequence alignments with other Cytolysins of the actinoporin family clearly indicated that Gigantoxin-4 belongs to this protein family. SDS-PAGE electrophoresis showed that this new actinoporin had a molecular mass of about 19 kDa, and possessed a high hemolytic activity to human erythrocytes (HA50= 40 ng/ml), which was inhibited by pre-incubation with sphingomyelin (SM) or SM-cholesterol mixtures. Our in vivo experiments showed that Gigantoxin-4 had wide toxicity to the rat cardiovascular system and the respiratory system. A concentration of 30?g/kg Gigantoxin-4, i.v. produced a positive inotropic effect on the rat heart although final cardiovascular failure was inevitable, and 60?g/kg Gigantoxin-4 caused respiratory arrest rapidly resulting in rat death. HE staining indicated pathological changes in various organs and tissues after i.v. administration of Gigantoxin-4. PMID:21697999

  20. Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis

    PubMed Central

    Amiel, Aldine R.; Johnston, Hereroa T.; Nedoncelle, Karine; Warner, Jacob F.; Ferreira, Solène; Röttinger, Eric

    2015-01-01

    Cnidarians, the extant sister group to bilateria, are well known for their impressive regenerative capacity. The sea anemone Nematostella vectensis is a well-established system for the study of development and evolution that is receiving increased attention for its regenerative capacity. Nematostella is able to regrow missing body parts within five to six days after its bisection, yet studies describing the morphological, cellular, and molecular events underlying this process are sparse and very heterogeneous in their experimental approaches. In this study, we lay down the basic framework to study oral regeneration in Nematostella vectensis. Using various imaging and staining techniques we characterize in detail the morphological, cellular, and global molecular events that define specific landmarks of this process. Furthermore, we describe in vivo assays to evaluate wound healing success and the initiation of pharynx reformation. Using our described landmarks for regeneration and in vivo assays, we analyze the effects of perturbing either transcription or cellular proliferation on the regenerative process. Interestingly, neither one of these experimental perturbations has major effects on wound closure, although they slightly delay or partially block it. We further show that while the inhibition of transcription blocks regeneration in a very early step, inhibiting cellular proliferation only affects later events such as pharynx reformation and tentacle elongation. PMID:26633371

  1. The genome of Aiptasia, a sea anemone model for coral symbiosis.

    PubMed

    Baumgarten, Sebastian; Simakov, Oleg; Esherick, Lisl Y; Liew, Yi Jin; Lehnert, Erik M; Michell, Craig T; Li, Yong; Hambleton, Elizabeth A; Guse, Annika; Oates, Matt E; Gough, Julian; Weis, Virginia M; Aranda, Manuel; Pringle, John R; Voolstra, Christian R

    2015-09-22

    The most diverse marine ecosystems, coral reefs, depend upon a functional symbiosis between a cnidarian animal host (the coral) and intracellular photosynthetic dinoflagellate algae. The molecular and cellular mechanisms underlying this endosymbiosis are not well understood, in part because of the difficulties of experimental work with corals. The small sea anemone Aiptasia provides a tractable laboratory model for investigating these mechanisms. Here we report on the assembly and analysis of the Aiptasia genome, which will provide a foundation for future studies and has revealed several features that may be key to understanding the evolution and function of the endosymbiosis. These features include genomic rearrangements and taxonomically restricted genes that may be functionally related to the symbiosis, aspects of host dependence on alga-derived nutrients, a novel and expanded cnidarian-specific family of putative pattern-recognition receptors that might be involved in the animal-algal interactions, and extensive lineage-specific horizontal gene transfer. Extensive integration of genes of prokaryotic origin, including genes for antimicrobial peptides, presumably reflects an intimate association of the animal-algal pair also with its prokaryotic microbiome. PMID:26324906

  2. Screening and cDNA Cloning of Kv1 Potassium Channel Toxins in Sea Anemones

    PubMed Central

    Yamaguchi, Yoshikazu; Hasegawa, Yuichi; Honma, Tomohiro; Nagashima, Yuji; Shiomi, Kazuo

    2010-01-01

    When 21 species of sea anemones were screened for Kv1 potassium channel toxins by competitive inhibition of the binding of 125I-α-dendrotoxin to rat synaptosomal membranes, 11 species (two species of Actiniidae, one species of Hormathiidae, five species of Stichodactylidae and three species of Thalassianthidae) were found to be positive. Furthermore, full-length cDNAs encoding type 1 potassium channel toxins from three species of Stichodactylidae and three species of Thalassianthidae were cloned by a combination of RT-PCR, 3′RACE and 5′RACE. The precursors of these six toxins are commonly composed of signal peptide, propart and mature peptide portions. As for the mature peptide (35 amino acid residues), the six toxins share more than 90% sequence identities with one another and with κ1.3-SHTX-She1a (Shk) from Stichodactyla helianthus but only 34–63% identities with the other type 1 potassium channel toxins. PMID:21339955

  3. Binding of sea anemone toxin to receptor sites associated with gating system of sodium channel in synaptic nerve endings in vitro.

    PubMed Central

    Vincent, J P; Balerna, M; Barhanin, J; Fosset, M; Lazdunski, M

    1980-01-01

    Iodination of toxin II from the sea anemone Anemonia sulcata gives a labeled monoiododerivative that retains 80% of the original neurotoxicity. This derivative binds specifically to rat brain synaptosomes at 20 degrees C and pH 7.4 with a second-order rate constant of association ka = 4.6 x 10(4) M-1 sec-1 and a first-order rate constant of dissociation kd = 1.1 x 10(-2) sec-1. The binding occurs on the Na+ channel at a binding site distinct from that of other gating system toxins like batrachotoxin, veratridine, grayanotoxin, aconitine, and pyrethroids. The maximal binding capacity Bmax is 3.2 pmol/mg of protein (i.e., about two sea anemone toxin binding sites per tetrodotoxin binding site) and the Kd is 240 nM for the monoiododerivative and 150 nM for the native toxin. Corresponding binding parameters for the association of a 125I-labeled derivative of toxin II from the scorpion Androctonus australis Hector are Bmax = 0.3 pmol/mg of protein and Kd = 1 nM, whereas the Kd of the unmodified scorpion toxin is 0.6 nM. Competition experiments involving scorpion toxins, sea anemone toxins, and synaptosomes demonstrate that, although the sea anemone toxin is able to displace the scorpion toxin bound to synaptosomes, the scorpion toxin does not displace the sea anemone toxin. The sea anemone toxin but not the scorpion toxin binds to depolarized synaptosomes. Differences between binding properties of the two polypeptide toxins are analyzed in the discussion. PMID:6103536

  4. Functional polarity of the tentacle of the sea anemone Anemonia viridis: role in inorganic carbon acquisition.

    PubMed

    Furla, P; Bénazet-Tambutté, S; Jaubert, J; Allemand, D

    1998-02-01

    The oral epithelial layers of anthozoans have a polarized morphology: photosynthetic endosymbionts live within endodermal cells facing the coelenteric cavity and are separated from the external seawater by the ectodermal layer and the mesoglea. To study if this morphology plays a role in the supply of inorganic carbon for symbiont photosynthesis, we measured the change in pH and the rate of OH- (H+) fluxes induced by each cell layer on a tentacle of the sea anemone Anemonia viridis. Light-induced pH increase of the medium bathing the endodermal layers led to the generation of a transepithelial pH gradient of approximately 0.8 pH units across the tentacle, whereas darkness induced acidification of this medium. The light-induced pH change was associated with an increase of total alkalinity. Only the endodermal layer was able to induce a net OH- secretion (H+ absorption). The light-induced OH- secretion by the endodermal cell layer was dependent on the presence of HCO3- in the compartment facing the ectoderm and was sensitive to several inhibitors of ion transport. [14C] HCO3- incorporation into photosynthates confirmed the ectodermal supply, the extent of which varied from 25 to > 90%, according to HCO3- availability. Our results suggest that the light-induced OH- secretion by the endodermal cell layer followed the polarized transport of HCO3- and its subsequent decarboxylation within the endodermal cell layer. This polarity may play a significant role both in inorganic carbon absorption and in the control of light-enhanced calcification in scleractinian corals. PMID:9486285

  5. Differential distribution of lipids in epidermis, gastrodermis and hosted Symbiodinium in the sea anemone Anemonia viridis.

    PubMed

    Revel, Johana; Massi, Lionel; Mehiri, Mohamed; Boutoute, Marc; Mayzaud, Patrick; Capron, Laure; Sabourault, Cécile

    2016-01-01

    Cnidarian-dinoflagellate symbiosis mainly relies on nutrient recycling, thus providing both partners with a competitive advantage in nutrient-poor waters. Essential processes related to lipid metabolism can be influenced by various factors, including hyperthermal stress. This can affect the lipid content and distribution in both partners, while contributing to symbiosis disruption and bleaching. In order to gain further insight into the role and distribution of lipids in the cnidarian metabolism, we investigated the lipid composition of the sea anemone Anemonia viridis and its photosynthetic dinoflagellate endosymbionts (Symbiodinium). We compared the lipid content and fatty acid profiles of the host cellular layers, non-symbiotic epidermal and symbiont-containing gastrodermal cells, and those of Symbiodinium, in a mass spectrometry-based assessment. Lipids were more concentrated in Symbiodinium cells, and the lipid class distribution was dominated by polar lipids in all tissues. The fatty acid distribution between host cell layers and Symbiodinium cells suggested potential lipid transfers between the partners. The lipid composition and distribution was modified during short-term hyperthermal stress, mainly in Symbiodinium cells and gastrodermis. Exposure to elevated temperature rapidly caused a decrease in polar lipid C18 unsaturated fatty acids and a strong and rapid decrease in the abundance of polar lipid fatty acids relative to sterols. These lipid indicators could therefore be used as sensitive biomarkers to assess the physiology of symbiotic cnidarians, especially the effect of thermal stress at the onset of cnidarian bleaching. Overall, the findings of this study provide some insight on key lipids that may regulate maintenance of the symbiotic interaction. PMID:26478191

  6. Mercury Distribution, Methylation and Volatilization in Microcosms with and without the Sea Anemone Bunodosoma caissarum

    NASA Astrophysics Data System (ADS)

    Ansari, N. R.; Correia, R. R. S.; Fernandez, M. A. S.; Cordeiro, R. C.; Guimarães, J. R. D.

    2014-12-01

    Mercury (Hg) can be a dangerous contaminant and has a complex biogeochemical cycling in aquatic environments. The sea anemone Bunodosoma caissarum is an endemic species in Brazil capable of bioaccumulating Hg from the ambient seawater. The radiotracer 203Hg was used in order to investigate mechanisms of Hg uptake and depuration of B. caissarum and the distribution of Hg in laboratory model systems, with and without B. caissarum. A single initial spike of 203Hg was added to each microcosm. Microcosms had continuous air renovation and trapping of Hg volatile forms. Total Hg in different compartments was measured by gamma spectrometry. In the uptake experiment 203Hg activity was determined periodically in seawater and specimens for 6 days. At the end, specimens had an average bioconcentration factor of 70. After the uptake experiment, methylmercury (MeHg) in seawater was extracted and measured by liquid scintillation. In microcosms with and without B. caissarum, respectively 0.05% and 0.32% of the initial spike was found as MeHg. Hg was probably less available for methylation in the first because of bioaccumulation and higher concentrations of suspended particulate matter that could form complexes with Hg. After that, specimens were transferred to unspiked microcosms. After a 48 day depuration specimens still retained 35 - 70% of the previously bioaccumulated Hg and 0.2 - 2.4% of the total Hg was MeHg. The presence of B. caissarum resulted in an unexpected higher volatilization of Hg (58%) compared to controls (17%). This increased volatilization is possibly a result of Hg2+ reduction mediated by microorganisms associated with its tissues and mucus secretions and/or an unknown defense mechanism of this species.

  7. The occurrence in and actions of amino acids on isolated supra oral sphincter preparations of the sea anemone Actinia equina

    PubMed Central

    Carlyle, R. F.

    1974-01-01

    1. L-glutamic acid reversibly inhibits the contractions of isolated circular muscle preparations from different body regions of the sea anemone Actinia equina to electrical stimulation. 2. L-glutamic acid does not cause inhibition of contraction by conversion to ammonia or ?-amino-butyric acid, but appears to act on a glutamate receptor. 3. It is postulated that in Actinia equina there is a receptor for glutamic acid whose structural requirements for activation are met by comparatively few analogues of glutamic acid. Of thirty-four analogues tested, only seven were active, most were less active, and only homocysteic acid had a greater activity than L-glutamic acid. 4. Glutamic acid does not depress conduction of contraction in circular muscle preparations. 5. The inhibition produced by L-glutamic acid does not appear to be conducted from one part of a preparation to another. 6. Glutamic acid occurs in high concentrations in supra oral sphincter preparations of Actinia equina. 7. Glutamic acid is released into sea water from sphincter preparations at rest and the amount released is increased by electrical stimulation of the preparation. However, many other amino acids are released at rest and following electrical stimulation, so that it is difficult to suggest that glutamic acid may be involved as a neurotransmitter in the sea anemone. PMID:4150801

  8. Upgrades to StellaBase facilitate medical and genetic studies on the starlet sea anemone, Nematostella vectensis.

    PubMed

    Sullivan, James C; Reitzel, Adam M; Finnerty, John R

    2008-01-01

    The starlet sea anemone, Nematostella vectensis, is a basal metazoan organism that has recently emerged as an important model system in developmental biology and evolutionary genomics. StellaBase, the Nematostella Genomics Database (http://stellabase.org), was developed in 2005 as a resource to support the Nematostella research community. Recently, it has become apparent that Nematostella may be a particularly useful system for studying (i) microevolutionary variation in natural populations, and (ii) the functional evolution of human disease genes. We have developed two new databases that will foster such studies: StellaBase Disease (http://stellabase.org/disease) is a relational database that houses 155 904 invertebrate homologous isoforms of human disease genes from four leading genomic model systems (fly, worm, yeast and Nematostella), including 14 874 predicted genes from the sea anemone itself. StellaBase SNP (http://stellabase.org/SNP) is a relational database that describes the location and underlying type of mutation for 20 063 single nucleotide polymorphisms. PMID:17982171

  9. Sea Anemone Peptide with Uncommon ?-Hairpin Structure Inhibits Acid-sensing Ion Channel 3 (ASIC3) and Reveals Analgesic Activity*

    PubMed Central

    Osmakov, Dmitry I.; Kozlov, Sergey A.; Andreev, Yaroslav A.; Koshelev, Sergey G.; Sanamyan, Nadezhda P.; Sanamyan, Karen E.; Dyachenko, Igor A.; Bondarenko, Dmitry A.; Murashev, Arkadii N.; Mineev, Konstantin S.; Arseniev, Alexander S.; Grishin, Eugene V.

    2013-01-01

    Three novel peptides were isolated from the venom of the sea anemone Urticina grebelnyi. All of them are 29 amino acid peptides cross-linked by two disulfide bridges, with a primary structure similar to other sea anemone peptides belonging to structural group 9a. The structure of the gene encoding the shared precursor protein of the identified peptides was determined. One peptide, ?-AnmTX Ugr 9a-1 (short name Ugr 9-1), produced a reversible inhibition effect on both the transient and the sustained current of human ASIC3 channels expressed in Xenopus laevis oocytes. It completely blocked the transient component (IC50 10 ± 0.6 ?m) and partially (48 ± 2%) inhibited the amplitude of the sustained component (IC50 1.44 ± 0.19 ?m). Using in vivo tests in mice, Ugr 9-1 significantly reversed inflammatory and acid-induced pain. The other two novel peptides, AnmTX Ugr 9a-2 (Ugr 9-2) and AnmTX Ugr 9a-3 (Ugr 9-3), did not inhibit the ASIC3 current. NMR spectroscopy revealed that Ugr 9-1 has an uncommon spatial structure, stabilized by two S-S bridges, with three classical ?-turns and twisted ?-hairpin without interstrand disulfide bonds. This is a novel peptide spatial structure that we propose to name boundless ?-hairpin. PMID:23801332

  10. Ancient origins of axial patterning genes: Hox genes and ParaHox genes in the Cnidaria.

    PubMed

    Finnerty, J R; Martindale, M Q

    1999-01-01

    Among the bilaterally symmetrical, triploblastic animals (the Bilateria), a conserved set of developmental regulatory genes are known to function in patterning the anterior-posterior (AP) axis. This set includes the well-studied Hox cluster genes, and the recently described genes of the ParaHox cluster, which is believed to be the evolutionary sister of the Hox cluster (Brooke et al. 1998). The conserved role of these axial patterning genes in animals as diverse as frogs and flies is believed to reflect an underlying homology (i.e., all bilaterians derive from a common ancestor which possessed an AP axis and the developmental mechanisms responsible for patterning the axis). However, the origin and early evolution of Hox genes and ParaHox genes remain obscure. Repeated attempts have been made to reconstruct the early evolution of Hox genes by analyzing data from the triphoblastic animals, the Bilateria (Schubert et al. 1993; Zhang and Nei 1996). A more precise dating of Hox origins has been elusive due to a lack of sufficient information from outgroup taxa such as the phylum Cnidaria (corals, hydras, jellyfishes, and sea anemones). In combination with outgroup taxa, another potential source of information about Hox origins is outgroup genes (e.g., the genes of the ParaHox cluster). In this article, we present cDNA sequences of two Hox-like genes (anthox2 and anthox6) from the sea anemone, Nematostella vectensis. Phylogenetic analysis indicates that anthox2 (= Cnox2) is homologous to the GSX class of ParaHox genes, and anthox6 is homologous to the anterior class of Hox genes. Therefore, the origin of Hox genes and ParaHox genes occurred prior to the evolutionary split between the Cnidaria and the Bilateria and predated the evolution of the anterior-posterior axis of bilaterian animals. Our analysis also suggests that the central Hox class was invented in the bilaterian lineage, subsequent to their split from the Cnidaria. PMID:11324016

  11. The influence of salinity and copper exposure on copper accumulation and physiological impairment in the sea anemone, Exaiptasia pallida.

    PubMed

    Patel, Pratikkumar P; Bielmyer-Fraser, Gretchen K

    2015-02-01

    Copper is a common pollutant in many aquatic environments, particularly those surrounding densely populated areas with substantial anthropogenic inputs. These same areas may also experience changes in salinity due to freshwater discharge and tidal influence. Biota that inhabit near-shore coastal environments may be susceptible to both stressors. Although copper is a noted concern in marine environments, effects of copper and varying salinity on symbiotic cnidarians are only scarcely studied. The sea anemone, Exaiptasia pallida, was used to investigate effects of copper on physiological impairment (i.e. activities of anti-oxidant enzymes) at two different salinities (20 and 25ppt). E. pallida was exposed to a control and three elevated copper concentrations for up to 21d, and copper accumulation and activity of the enzymes: catalase, glutathione reductase, glutathione peroxidase, and carbonic anhydrase were measured in the anemones. Photosynthetic parameters in E. pallida's symbiotic dinoflagellate algae were also quantified. Over the course of the exposure, E. pallida accumulated copper in a concentration-dependent manner. Higher tissue copper concentrations were observed in anemones exposed to the lower salinity water (20ppt), and physiological impairment was observed as a consequence of both increased copper exposure and decreased salinity; however, changes in salinity caused a greater response than copper exposure, at the levels tested. In general, antioxidant enzyme activity increased as a consequence of decreased salinity and/or increased copper exposure. These results clearly demonstrated the influence of two local stressors, at environmentally realistic concentrations, on a sensitive cnidarian, and highlight the importance of characterizing combined exposure scenarios. PMID:25451077

  12. From Sea Anemone to Homo sapiens: The Evolution of the p53 Family of Genes

    SciTech Connect

    Levine, Arnold

    2009-09-14

    The human genome contains three transcription factors termed p53, p63 and p73 which are related orthologues. The function of the p53 protein is to respond to a wide variety of stresses which can disrupt the fidelity of DNA replication and cell division in somatic cells of the body. These stress signals, such as DNA damage, increase the mutation rate during DNA duplication and so an active p53 protein responds by eliminating clones of cells with mutations employing apoptosis, senescence or cell cycle arrest. In this way the p53 protein acts as a tumor suppressor preventing the mutations that can lead to cancers. The p63 and p73 proteins act in a similar fashion to protect the germ line cells in females (eggs). In addition the p63 protein plays a central role in the formation of epithelial cell layers and p73 plays a critical role in the formation of several structures in the central nervous system. Based upon their amino acid sequences and structural considerations the oldest organisms that contain an ancestor of the p53/p63/p73 gene are the sea anemone or hydra. The present day representatives of these animals contain a p63/p73 like ancestor gene and the protein functions in germ cells of this animal to enforce the fidelity of DNA replication after exposure to ultraviolet light. Thus the structure and functions of this gene family have been preserved for over one billion years of evolution. Other invertebrates such as the worm, the fly and the clam contain a very similar ancestor gene with a similar set of functions. The withdrawal of a food source from a worm results in the p63/p73 mediated apoptosis of the eggs so that new organisms will not be hatched into a poor environment. A similar response is thought to occur in humans. Thus this ancestor gene ensures the fidelity of the next generation of organisms. The first time a clearly distinct new p53 gene arises is in the cartilaginous fish and in the bony fish a separation of the p

  13. From Sea Anemone to Homo Sapiens: The Evolution of the p53 Family of Genes

    SciTech Connect

    Levine, Arnold

    2009-09-14

    The human genome contains three transcription factors termed p53, p63 and p73 which are related orthologues. The function of the p53 protein is to respond to a wide variety of stresses which can disrupt the fidelity of DNA replication and cell division in somatic cells of the body. These stress signals, such as DNA damage, increase the mutation rate during DNA duplication and so an active p53 protein responds by eliminating clones of cells with mutations employing apoptosis, senescence or cell cycle arrest. In this way the p53 protein acts as a tumor suppressor preventing the mutations that can lead to cancers. The p63 and p73 proteins act in a similar fashion to protect the germ line cells in females (eggs). In addition the p63 protein plays a central role in the formation of epithelial cell layers and p73 plays a critical role in the formation of several structures in the central nervous system. Based upon their amino acid sequences and structural considerations the oldest organisms that contain an ancestor of the p53/p63/p73 gene are the sea anemone or hydra. The present day representatives of these animals contain a p63/p73 like ancestor gene and the protein functions in germ cells of this animal to enforce the fidelity of DNA replication after exposure to ultraviolet light. Thus the structure and functions of this gene family have been preserved for over one billion years of evolution. Other invertebrates such as the worm, the fly and the clam contain a very similar ancestor gene with a similar set of functions. The withdrawal of a food source from a worm results in the p63/p73 mediated apoptosis of the eggs so that new organisms will not be hatched into a poor environment. A similar response is thought to occur in humans. Thus this ancestor gene ensures the fidelity of the next generation of organisms. The first time a clearly distinct new p53 gene arises is in the cartilaginous fish and in the bony fish a separation of the p

  14. Expression and mutagenesis of the sea anemone toxin Av2 reveals key amino acid residues important for activity on voltage-gated sodium channels.

    PubMed

    Moran, Yehu; Cohen, Lior; Kahn, Roy; Karbat, Izhar; Gordon, Dalia; Gurevitz, Michael

    2006-07-25

    Type I sea anemone toxins are highly potent modulators of voltage-gated Na-channels (Na(v)s) and compete with the structurally dissimilar scorpion alpha-toxins on binding to receptor site-3. Although these features provide two structurally different probes for studying receptor site-3 and channel fast inactivation, the bioactive surface of sea anemone toxins has not been fully resolved. We established an efficient expression system for Av2 (known as ATX II), a highly insecticidal sea anemone toxin from Anemonia viridis (previously named A. sulcata), and mutagenized it throughout. Each toxin mutant was analyzed in toxicity and binding assays as well as by circular dichroism spectroscopy to discern the effects derived from structural perturbation from those related to bioactivity. Six residues were found to constitute the anti-insect bioactive surface of Av2 (Val-2, Leu-5, Asn-16, Leu-18, and Ile-41). Further analysis of nine Av2 mutants on the human heart channel Na(v)1.5 expressed in Xenopus oocytes indicated that the bioactive surfaces toward insects and mammals practically coincide but differ from the bioactive surface of a structurally similar sea anemone toxin, Anthopleurin B, from Anthopleura xanthogrammica. Hence, our results not only demonstrate clear differences in the bioactive surfaces of Av2 and scorpion alpha-toxins but also indicate that despite the general conservation in structure and importance of the Arg-14 loop and its flanking residues Gly-10 and Gly-20 for function, the surface of interaction between different sea anemone toxins and Na(v)s varies. PMID:16846229

  15. Symbiodinium diversity in the sea anemone Entacmaea quadricolor on the east Australian coast

    NASA Astrophysics Data System (ADS)

    Pontasch, S.; Scott, A.; Hill, R.; Bridge, T.; Fisher, P. L.; Davy, S. K.

    2014-06-01

    The diversity of Symbiodinium spp. in Entacmaea quadricolor was analysed from five locations along ~2,100 km on the east coast of Australia using denaturing gradient gel electrophoresis (DGGE) of the internal transcribed spacer 2 region (ITS2) combined with bacterial cloning. DGGE revealed that E. quadricolor predominantly associated with six types of clade C (four of which are novel) and that most anemones harboured multiple types simultaneously. Anemones from southern locations associated with a mixed assemblage of C25 and a variant of C3. This assemblage also dominated the central location, but was absent at the northern location. At central and northern sites, two novel variants of C42(type2) and C1 were found. Anemones hosting C42(type2) also showed a low abundance of variants of C3 and C1, and E1 was found in one sample, as revealed by bacterial cloning. The occurrence of geographically distinct ITS2 types or a consortium of types might reflect a need to optimise physiological performance of the symbiosis at different latitudes.

  16. Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones

    PubMed Central

    Urbarova, Ilona; Karlsen, Bård Ove; Okkenhaug, Siri; Seternes, Ole Morten; Johansen, Steinar D.; Emblem, Åse

    2012-01-01

    Marine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the transcriptomes from the adult polyp stage of two cold-water sea anemones, Bolocera tuediae and Hormathia digitata. We generated approximately 1.1 million quality-filtered sequencing reads by 454 pyrosequencing, which were assembled into approximately 120,000 contigs and 220,000 single reads. Based on annotation and gene ontology analysis we profiled the expressed mRNA transcripts according to known biological processes. As a proof-of-concept we identified polypeptide toxins with a potential blocking activity on sodium and potassium voltage-gated channels from digital transcriptome libraries. PMID:23170083

  17. The feeding habits of three Mediterranean sea anemone species, Anemonia viridis (Forskål), Actinia equina (Linnaeus) and Cereus pedunculatus (Pennant)

    NASA Astrophysics Data System (ADS)

    Chintiroglou, Ch.; Koukouras, A.

    1992-03-01

    The feeding habits of the Mediterranean sea anemones Cereus pedunculatus, Actinia equina and Anemonia viridis were examined mainly by analysing their coelenteron contents. The three species are opportunistic omnivorous suspension feeders. Main source of food for A. viridis and C. pedunculatus are crustaceans (mainly amphipods and decapods, respectively), while for the midlittoral species A. equina, it is organic detritus. Using the same method, the temporal and spatial changes in the diet of A. viridis were examined. During the whole year, crustaceans seem to be the main source of food for A. viridis. The diet composition of this species, however, differs remarkably in space, possibly reflecting the different composition of the macrobenthic organismic assemblages in different areas. The data collected are compared with the limited bibliographical information.

  18. Digital marine bioprospecting: mining new neurotoxin drug candidates from the transcriptomes of cold-water sea anemones.

    PubMed

    Urbarova, Ilona; Karlsen, Bård Ove; Okkenhaug, Siri; Seternes, Ole Morten; Johansen, Steinar D; Emblem, Ase

    2012-10-01

    Marine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the transcriptomes from the adult polyp stage of two cold-water sea anemones, Bolocera tuediae and Hormathia digitata. We generated approximately 1.1 million quality-filtered sequencing reads by 454 pyrosequencing, which were assembled into approximately 120,000 contigs and 220,000 single reads. Based on annotation and gene ontology analysis we profiled the expressed mRNA transcripts according to known biological processes. As a proof-of-concept we identified polypeptide toxins with a potential blocking activity on sodium and potassium voltage-gated channels from digital transcriptome libraries. PMID:23170083

  19. In silico assessment of interaction of sea anemone toxin APETx2 and acid sensing ion channel 3

    SciTech Connect

    Rahman, Taufiq Smith, Ewan St. John

    2014-07-18

    Highlights: • We have made a reasonable model of rat ASIC3 using published structure of chicken ASIC1. • We have docked sea anemone toxin APETx2 on the model. • We have identified two putative sites for toxin binding. • We have argued for plausibility one site over the other. • We have identified the residues that are likely to be critical for APETx2–ASIC3 interaction. - Abstract: Acid sensing ion channels (ASICs) are proton-gated cation channels that are expressed throughout the nervous system and have been implicated in mediating sensory perception of noxious stimuli. Amongst the six ASIC isoforms, ASIC1a, 1b, 2a and 3 form proton-gated homomers, which differ in their activation and inactivation kinetics, expression profiles and pharmacological modulation; protons do not gate ASIC2b and ASIC4. As with many other ion channels, structure-function studies of ASICs have been greatly aided by the discovery of some toxins that act in isoform-specific ways. ASIC3 is predominantly expressed by sensory neurons of the peripheral nervous system where it acts to detect acid as a noxious stimulus and thus plays an important role in nociception. ASIC3 is the only ASIC subunit that is inhibited by the sea anemone (Anthopleura elegantissima)-derived toxin APETx2. However, the molecular mechanism by which APETx2 interacts with ASIC3 remains largely unknown. In this study, we made a homology model of ASIC3 and used extensive protein–protein docking to predict for the first time, the probable sites of APETx2 interaction on ASIC3. Additionally, using computational alanine scanning, we also suggest the ‘hot-spots’ that are likely to be critical for ASIC3–APETx2 interaction.

  20. Predicting suitable habitat for the gold coral Savalia savaglia (Bertoloni, 1819) (Cnidaria, Zoantharia) in the South Tyrrhenian Sea

    NASA Astrophysics Data System (ADS)

    Giusti, Michela; Innocenti, Carlo; Canese, Simonepietro

    2014-06-01

    The gold coral Savalia savaglia (Cnidaria, Zoantharia) is a rare component of the mesophotic zone of the Mediterranean Sea and northeastern Atlantic Ocean. During two field campaigns along the Italian coast in the South Tyrrhenian Sea, two populations of this species were discovered. The specimens were filmed and photographed by means of a Remotely Operated Vehicle (ROV). To identify the role of local bathymetry and other derived variables on presence and distribution of S. savaglia we used a Habitat Suitability (HS) modeling technique based on Ecological Niche Factor Analysis (ENFA), utilizing high-resolution multibeam bathymetry and ROV data. Among the set of environmental variables derived from multibeam data, slope, rugosity, eastness and distance to rocks, appear to be the main variables involved in S. savaglia distribution, pointing out that the habitat differs considerably from the mean environmental conditions over the study area, and that S. savaglia ecological niche is significantly narrower than the available habitat. The HS map was developed to differentiate the sea floor into suitability classes. The comparison between suitability classes and presence data showed that the HS map is coherent with the observed spatial distribution of the species. The most suitable habitat for S. savaglia is characterized by a rough sea floor with rocks that is steeply sloped, oriented northeast, and within a water depth range of 34-77 m. Our study suggests that predictive modeling is an approach that can be applied to other deep coral species to locate areas with a suitable habitat. Considering the difficulties to reach the habitats in which these species live, this approach could be essential to planning further studies that help define areas where the species may be present.

  1. The 60-kDa heat shock protein (HSP60) of the sea anemone Anemonia viridis: a potential early warning system for environmental changes.

    PubMed

    Choresh, O; Ron, E; Loya, Y

    2001-09-01

    Expression of heat shock proteins (HSPs) is often correlated with adaptation to environmental stress. We examined the role of HSP60 (60 kDa) in acclimatization to thermal stress in the sea anemone Anemonia viridis. Using monoclonal antibodies, we identified HSP60 in sea anemones for the first time, and showed that its expression varied with changes in seawater temperature (SWT). Anemonia viridis displayed high levels of HSP60 when extreme temperatures prevailed in stressful habitats such as tidal pools. Specimens sampled from different temperature layers in the same tidal pool differed in their levels of HSP60. Specimens from subtidal zones exhibited a seasonal pattern of expression of HSP60, according to the seasonal SWT. The level of HSP60 was significantly higher in the summer (SWT, 31 degrees C) than in other seasons throughout the year. This study suggests the use of HSP60 expression as a tool for stress detection in marine invertebrates. PMID:14961344

  2. Daily cycle in oxygen consumption by the sea anemone Nematostella vectensis Stephenson.

    PubMed

    Maas, Amy E; Jones, Ian T; Reitzel, Adam M; Tarrant, Ann M

    2016-01-01

    In bilaterian animals, the circadian clock is intimately involved in regulating energetic metabolism. Although cnidarians exhibit diel behavioral rhythms including cycles in locomotor activity, tentacle extension and spawning, daily cycles in cnidarian metabolism have not been described. To explore a possible circadian metabolic cycle, we maintained the anemone Nematostella vectensis in a 12?h light/dark cycle, a reversed light cycle, or in constant darkness. Oxygen consumption rates were measured at intervals using an optical oxygen meter. Respiration rates responded to entrainment with higher rates during light periods. During a second experiment with higher temporal resolution, respiration rates peaked late in the light period. The diel pattern could be detected after six days in constant darkness. Together, our results suggest that respiration rates in Nematostella exhibit a daily cycle that may be under circadian control and that the cycle in respiration rate is not driven by the previously described nocturnal increase in locomotor activity in this species. PMID:26772201

  3. Variation in partner benefits in a shrimp—sea anemone symbiosis

    PubMed Central

    O’Donnell, James L.

    2015-01-01

    Symbiotic interactions, where two species occur in close physical proximity for the majority of the participants’ lifespans, may constrain the fitness of one or both of the participants. Host choice could result in lineage divergence in symbionts if fitness benefits vary across the interaction with hosts. Symbiotic interactions are common in the marine environment, particularly in the most diverse marine ecosystems: coral reefs. However, the variation in symbiotic interactions that may drive diversification is poorly understood in marine systems. We measured the fecundity of the symbiotic shrimp Periclimenes yucatanicus on two anemone hosts on coral reefs in Panama, and found that while fecundity varies among host species, this variation is explained largely by host size, not species. This suggests that shrimp on larger hosts may have higher fitness regardless of host species, which in turn could drive selection for host choice, a proposed driver of diversification in this group. PMID:26618082

  4. Exploiting the Nephrotoxic Effects of Venom from the Sea Anemone, Phyllodiscus semoni, to Create a Hemolytic Uremic Syndrome Model in the Rat

    PubMed Central

    Mizuno, Masashi; Ito, Yasuhiko; Morgan, B. Paul

    2012-01-01

    In the natural world, there are many creatures with venoms that have interesting and varied activities. Although the sea anemone, a member of the phylum Coelenterata, has venom that it uses to capture and immobilise small fishes and shrimp and for protection from predators, most sea anemones are harmless to man. However, a few species are highly toxic; some have venoms containing neurotoxins, recently suggested as potential immune-modulators for therapeutic application in immune diseases. Phyllodiscus semoni is a highly toxic sea anemone; the venom has multiple effects, including lethality, hemolysis and renal injuries. We previously reported that venom extracted from Phyllodiscus semoni induced acute glomerular endothelial injuries in rats resembling hemolytic uremic syndrome (HUS), accompanied with complement dysregulation in glomeruli and suggested that the model might be useful for analyses of pathology and development of therapeutic approaches in HUS. In this mini-review, we describe in detail the venom-induced acute renal injuries in rat and summarize how the venom of Phyllodiscus semoni could have potential as a tool for analyses of complement activation and therapeutic interventions in HUS. PMID:22851928

  5. Anemonefish oxygenate their anemone hosts at night.

    PubMed

    Szczebak, Joseph T; Henry, Raymond P; Al-Horani, Fuad A; Chadwick, Nanette E

    2013-03-15

    Many stony coral-dwelling fishes exhibit adaptations to deal with hypoxia among the branches of their hosts; however, no information exists on the respiratory ecophysiology of obligate fish associates of non-coral organisms such as sea anemones and sponges. This study investigated metabolic and behavioral interactions between two-band anemonefish (Amphiprion bicinctus) and bulb-tentacle sea anemones (Entacmaea quadricolor) at night. We measured the net dark oxygen uptake ( , ?mol O2 h(-1)) of fish-anemone pairs when partners were separate from each other, together as a unit, and together as a unit but separated by a mesh screen that prevented physical contact. We also measured the effects of water current on sea anemone and quantified the nocturnal behaviors of fish in the absence and presence of host anemones in order to discern the impacts of anemone presence on fish behavior. Net of united pairs was significantly higher than that of both separated pairs and united pairs that were separated by a mesh screen. Anemone increased with flow rate from 0.5 to 2.0 cm s(-1), after which remained constant up to a water flow rate of 8.0 cm s(-1). Furthermore, the percentage time and bout frequency of flow-modulating behaviors by fish increased significantly when anemones were present. We conclude that physical contact between anemonefish and sea anemones elevates the of at least one of the partners at night, and anemonefish behavior at night appears to oxygenate sea anemone hosts and to augment the metabolism of both partners. PMID:23447664

  6. Optimization of preservation and processing of sea anemones for microbial community analysis using molecular tools.

    PubMed

    Rocha, Joana; Coelho, Francisco J R C; Peixe, Luísa; Gomes, Newton C M; Calado, Ricardo

    2014-01-01

    For several years, knowledge on the microbiome associated with marine invertebrates was impaired by the challenges associated with the characterization of bacterial communities. With the advent of culture independent molecular tools it is possible to gain new insights on the diversity and richness of microorganisms associated with marine invertebrates. In the present study, we evaluated if different preservation and processing methodologies (prior to DNA extraction) can affect the bacterial diversity retrieved from snakelocks anemone Anemonia viridis. Denaturing gradient gel electrophoresis (DGGE) community fingerprints were used as proxy to determine the bacterial diversity retrieved (H'). Statistical analyses indicated that preservation significantly affects H'. The best approach to preserve and process A. viridis biomass for bacterial community fingerprint analysis was flash freezing in liquid nitrogen (preservation) followed by the use of a mechanical homogenizer (process), as it consistently yielded higher H'. Alternatively, biomass samples can be processed fresh followed by cell lyses using a mechanical homogenizer or mortar &pestle. The suitability of employing these two alternative procedures was further reinforced by the quantification of the 16S rRNA gene; no significant differences were recorded when comparing these two approaches and the use of liquid nitrogen followed by processing with a mechanical homogenizer. PMID:25384534

  7. Internal brooding favours pre-metamorphic chimerism in a non-colonial cnidarian, the sea anemone Urticina felina

    PubMed Central

    Mercier, Annie; Sun, Zhao; Hamel, Jean-François

    2011-01-01

    The concept of intraorganismal genetic heterogeneity resulting from allogeneic fusion (i.e. chimerism) has almost exclusively been explored in modular organisms that have the capacity to reproduce asexually, such as colonial ascidians and corals. Apart from medical conditions in mammals, the natural development of chimeras across ontogenetic stages has not been investigated in any unitary organism incapable of asexual propagation. Furthermore, chimerism was mainly studied among gregarious settlers to show that clustering of genetically similar individuals upon settlement promotes the occurrence of multi-chimeras exhibiting greater fitness. The possible occurrence of chimeric embryos and larvae prior to settlement has not received any attention. Here we document for the first time the presence of natural chimeras in brooded embryos and larvae of a unitary cnidarian, the sea anemone Urticina felina. Rates of visible bi- and multi-chimerism of up to 3.13 per cent were measured in the broods of 16 females. Apart from these sectorial chimeras, monitored fusion events also yielded homogeneous chimeric entities (mega-larvae) suggesting that the actual rates of natural chimerism in U. felina are greater than predicted by visual assessment. In support of this assumption, the broods of certain individuals comprised a dominant proportion (to 90%) of inexplicably large embryos and larvae (relative to oocyte size). Findings of fusion and chimerism in a unitary organism add a novel dimension to the framework within which the mechanisms and evolutionary significance of genetic heterogeneity in animal taxa can be explored. PMID:21508035

  8. Sequence-specific H NMR assignments and secondary structure in the sea anemone polypeptide Stichodactyla helianthus neurotoxin I

    SciTech Connect

    Fogh, R.H.; Mabbutt, B.C.; Kem, W.R.; Norton, R.S. )

    1989-02-21

    Sequence-specific assignments are reported for the 500-MHz H nuclear magnetic resonance (NMR) spectrum of the 48-residue polypeptide neurotoxin I from the sea anemone Stichodactyla helianthus (Sh I). Spin systems were first identified by using two-dimensional relayed or multiple quantum filtered correlation spectroscopy, double quantum spectroscopy, and spin lock experiments. Specific resonance assignments were then obtained from nuclear Overhauser enhancement (NOE) connectivities between protons from residues adjacent in the amino acid sequence. Of a total of 265 potentially observable resonances, 248 (i.e., 94%) were assigned, arising from 39 completely and 9 partially assigned amino acid spin systems. The secondary structure of Sh I was defined on the basis of the pattern of sequential NOE connectivities. NOEs between protons on separate strands of the polypeptide backbone, and backbone amide exchange rates. Sh I contains a four-stranded antiparallel {beta}-sheet encompassing residues 1-5, 16-24, 30-33, and 40-46, with a {beta}-bulge at residues 17 and 18 and a reverse turn, probably a type II {beta}-turn, involving residues 27-30. No evidence of {alpha}-helical structure was found.

  9. Changes in microbial communities associated with the sea anemone Anemonia viridis in a natural pH gradient.

    PubMed

    Meron, Dalit; Buia, Maria-Cristina; Fine, Maoz; Banin, Ehud

    2013-02-01

    Ocean acidification, resulting from rising atmospheric carbon dioxide concentrations, is a pervasive stressor that can affect many marine organisms and their symbionts. Studies which examine the host physiology and microbial communities have shown a variety of responses to the ocean acidification process. Recently, several studies were conducted based on field experiments, which take place in natural CO(2) vents, exposing the host to natural environmental conditions of varying pH. This study examines the sea anemone Anemonia viridis which is found naturally along the pH gradient in Ischia, Italy, with an aim to characterize whether exposure to pH impacts the holobiont. The physiological parameters of A. viridis (Symbiodinium density, protein, and chlorophyll a+c concentration) and its microbial community were monitored. Although reduction in pH was seen to have had an impact on composition and diversity of associated microbial communities, no significant changes were observed in A. viridis physiology, and no microbial stress indicators (i.e., pathogens, antibacterial activity, etc.) were detected. In light of these results, it appears that elevated CO(2) does not have a negative influence on A. viridis that live naturally in the site. This suggests that natural long-term exposure and dynamic diverse microbial communities may contribute to the acclimation process of the host in a changing pH environment. PMID:23011286

  10. The transcriptomic response to thermal stress is immediate, transient and potentiated by ultraviolet radiation in the sea anemone Anemonia viridis.

    PubMed

    Moya, A; Ganot, P; Furla, P; Sabourault, C

    2012-03-01

    Among the environmental threats to coral reef health, temperature and ultraviolet increases have been proposed as major agents, although the relative contribution of each in the cnidarian/zooxanthellae symbiosis breakdown has been poorly addressed. We have investigated the transcriptomic response to thermal stress, with and without ultraviolet radiation (UVR), in the symbiotic sea anemone Anemonia viridis. Using the Oligo2K A. viridis microarray, dedicated to genes potentially involved in the symbiosis interaction, we monitored the gene expression profiles after 1, 2 and 5?days of stresses that further lead to massive losses of zooxanthellae. Each stress showed a specific gene expression profile with very little overlap. We showed that the major response to thermal stress is immediate (24?h) but returns to the baseline gene expression profile after 2?days. UVR alone has little effect but potentiates thermal stress, as a second response at 5?days was observed when the two stresses were coupled. Several pathways were highlighted, such as mesoglea loosening, cell death and calcium homeostasis and described in more details. Finally, we showed that the dermatopontin gene family, potentially involved in collagen fibrillogenesis, issued from actinarian-specific duplication events, with one member preferentially expressed in the gastroderm and specifically responding to stress. Anemonia viridis EST sequences have been deposited into GenBank dbEST ([GenBank:FK719875–FK759813]. PMID:22288383

  11. Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis

    PubMed Central

    Layden, Michael J; Röttinger, Eric; Wolenski, Francis S; Gilmore, Thomas D; Martindale, Mark Q

    2016-01-01

    We describe a protocol for microinjection of embryos for an emerging model system, the cnidarian sea anemone, Nematostella vectensis. In addition, we provide protocols for carrying out overexpression and knockdown of gene function through microinjection of in vitro–translated mRNAs or gene-specific oligonucleotide morpholinos (Mos), respectively. our approach is simple, and it takes advantage of the natural adherence properties of the early embryo to position them in a single layer on a polystyrene dish. embryos are visualized on a dissecting microscope equipped with epifluorescence and injected with microinjection needles using a picospritzer forced-air injection system. a micromanipulator is used to guide the needle to impale individual embryos. Injection takes ∼1.5 h, and an experienced researcher can inject ∼2,000 embryos in a single session. With the availability of the published Nematostella genome, the entire protocol, including cloning and transcription of mRNAs, can be carried out in ∼1 week. PMID:23579781

  12. The effect of sea anemone (H. magnifica) venom on two human breast cancer lines: death by apoptosis.

    PubMed

    Ramezanpour, Mahnaz; da Silva, Karen Burke; Sanderson, Barbara J S

    2014-10-01

    Venom from the sea anemone, Heteractis magnifica, has multiple biological effects including, cytotoxic, cytolytic and hemolytic activities. In this study, cytotoxicity induced by H. magnifica venom was investigated using the crystal violet assay on human breast cancer T47D and MCF7 cell lines and normal human breast 184B5 cell line. Apoptosis was also assayed via Annexin V-flourescein isothiocyanate and propidium iodide (PI) staining followed by flow cytometric analysis. Cell cycle progression and mitochondria membrane potential were studied via flow cytometry following PI and JC-1 staining respectively. H. magnifica venom induced significant reductions in viable cell numbers and increases in apoptosis in T47D and MCF7 in dose-dependent manners. A significant apoptosis-related increase in the sub G1 peak of the cell cycle in both breast cancer cell lines was also observed. Moreover, treatment by venom cleaved caspase-8, caspase-9, and activated caspase-3. Overall, H. magnifica venom was highly cytotoxic to T47D and MCF7 human breast cancer cells, and the phenomenon could be the killing phenomenon via the death receptor-mediated and the mitochondria-mediated apoptotic pathways. Consequently, H. magnifica venom has potential for the development of a breast cancer therapeutic. PMID:23989939

  13. The making of an embryo in a basal metazoan: Proteomic analysis in the sea anemone Nematostella vectensis.

    PubMed

    Levitan, Shimrit; Sher, Noa; Brekhman, Vera; Ziv, Tamar; Lubzens, Esther; Lotan, Tamar

    2015-12-01

    Cnidarians are widely distributed basal metazoans that play an important role in the marine ecosystem. Their genetic diversity and dispersal depends on successful oogenesis, fertilization and embryogenesis. To understand the processes that lead to successful embryogenesis in these basal organisms, we conducted comparative proteomics on the model sea anemone Nematostella vectensis. We examined four developmental stages from oocyte maturation through early embryogenesis, as well as the oocyte jelly sac in which fertilization and embryogenesis take place. Our analysis revealed 37 stage-specifically expressed proteins, including cell cycle, cellular energy related and DNA replication proteins and transcription regulators. Using in situ hybridization, we show that within the mesenteria, two cell types support successful oocyte development and embryogenesis. Large somatic supporting cells synthesize vitellogenin, the most abundant egg yolk protein within the oocyte, whereas mesenteria gland cells synthesize mucin 5B, which was found to be the main component of the jelly sac. These findings shed light on the sexual reproduction program in cnidarians and suggest a high conservation with proteins governing oogenesis in Bilateria. PMID:26365810

  14. Evaluation of the sea anemone Anthothoe albocincta as an augmentative biocontrol agent for biofouling on artificial structures.

    PubMed

    Atalah, Javier; Bennett, Holly; Hopkins, Grant A; Forrest, Barrie M

    2013-01-01

    Augmentative biocontrol, defined as the use of indigenous natural enemies to control pest populations, has not been explored extensively in marine systems. This study tested the potential of the anemone Anthothoe albocincta as a biocontrol agent for biofouling on submerged artificial structures. Biofouling biomass was negatively related to anemone cover. Treatments with high anemone cover (>35%) led to significant changes in biofouling assemblages compared to controls. Taxa that contributed to these changes differed among sites, but included reductions in cover of problematic fouling organisms, such as solitary ascidians and bryozoans. In laboratory trials, A. albocincta substantially prevented the settlement of larvae of the bryozoan Bugula neritina when exposed to three levels of larval dose, suggesting predation as an important biocontrol mechanism, in addition to space pre-emption. This study demonstrated that augmentative biocontrol using anemones has the potential to reduce biofouling on marine artificial structures, although considerable further work is required to refine this tool before its application. PMID:23682610

  15. The phylum Cnidaria and investigations of its toxins and venoms until 1990.

    PubMed

    Turk, Tom; Kem, William R

    2009-12-15

    Cnidarians are the largest phylum of generally toxic animals, yet their toxins and venoms have not received as much scientific attention as those of many terrestrial (snakes, scorpions, spiders, etc.) and even some marine animals (i.e. cone snails). Approximately 13,000 living cnidarian species have been described by systematists. A major rationale for their study in the past, besides scientific curiosity, was to better treat victims of their envenomation. While that goal remains a high priority, it is now appreciated that the toxins of these mostly marine animals can be very useful molecular probes for the analysis of ion channels involved in electrical signaling, immune responses and other signal transduction processes of biomedical interest. For instance, anaphylaxis was discovered by Richet (1905) during experiments with sea anemone and hydrozoan tentacular extracts. Similarly, it has recently been shown that a toxin from another sea anemone is able to potently inhibit T-lymphocyte proliferation in models of certain autoimmune diseases. Thus, these natural substances continue to be of relevance for understanding and treating human diseases. In addition to introducing phylum Cnidaria (Coelenterata), we provide a short history of early (until about 1990) research on cnidarian toxins and venoms, to provide a perspective for appreciating the scientific advances of the past two decades that are summarized in the ensuing 19 papers in this special Toxicon issue. PMID:19576920

  16. The influence of UV radiation on number and ultrastructure of the endosymbiotic dinoflagellates in the sea anemone Cereus pedunculatus (Anthozoa: Actiniaria)

    NASA Astrophysics Data System (ADS)

    Hannack, K.; Kestler, P.; Sicken, O.; Westheide, W.

    1998-02-01

    The sea anemone Cereus pedunculatus was artificially UV-irradiated to test the effect of UV-light on the number of endosymbiotic dinoflagellates in its gastrodermis and on their ultrastructure. Anemones were kept in the laboratory in a light: dark cycle (LD 12?12; 13 W m-2) at 18 °C and briefly (2, 5 and 9 d) exposed to UV radiation at quasisolar intensities, 0.5 or 1 W m-2. Their tentacles were then examined in the electron microscope for qualitative and quantitative changes in the zooxanthellae. There was an intensity-dependent decrease in the number of symbionts, which in some cases were lost altogether (bleaching). Irradiated anemones contained a larger proportion of symbionts with ultrastructural abnormalities, namely diminished starch, some mitochondria with altered matrix and, in particular, characteristic changes in the chloroplasts; instead of being densely stacked, the thylakoids were spread apart and swollen at the ends of their membranes to form vesicle-like structures. Relatively large vesicles also appeared in the cytoplasm. The resulting enlargement of the whole dinoflagellate cell was documented morphometrically. Another intensity-dependent effect was a significant decrease in mitosis rate, established by counting dividing symbiont cells in TEM micrographs. *** DIRECT SUPPORT *** A03B6037 00006

  17. The Evolution of MicroRNA Pathway Protein Components in Cnidaria

    PubMed Central

    Moran, Yehu; Praher, Daniela; Fredman, David; Technau, Ulrich

    2013-01-01

    In the last decade, it became evident that posttranscriptional regulation of gene expression by microRNAs is a central biological process in both plants and animals. Yet, our knowledge about microRNA biogenesis and utilization in animals stems mostly from the study of Bilateria. In this study, we identified genes encoding the protein components of different parts of the microRNA pathway in Cnidaria, the likely sister phylum of Bilateria. These genes originated from three cnidarian lineages (sea anemones, stony corals, and hydras) that are separated by at least 500 My from one another. We studied the expression and phylogeny of the cnidarian homologs of Drosha and Pasha (DGCR8) that compose the microprocessor, the RNAse III enzyme Dicer and its partners, the HEN1 methyltransferase, the Argonaute protein effectors, as well as members of the GW182 protein family. We further reveal that whereas the bilaterian dicer partners Loquacious/TRBP and PACT are absent from Cnidaria, this phylum contains homologs of the double-stranded RNA-binding protein HYL1, the Dicer partner found in plants. We also identified HYL1 homologs in a sponge and a ctenophore. This finding raises questions regarding the independent evolution of the microRNA pathway in plants and animals, and together with the other results shed new light on the evolution of an important regulatory pathway. PMID:24030553

  18. Mechanical and electrophysiological effects of sea anemone (Anemonia sulcata) toxins on rat innervated and denervated skeletal muscle.

    PubMed Central

    Alsen, C.; Harris, J. B.; Tesseraux, I.

    1981-01-01

    1 Some effects of the sea-anemone toxin ATX-II on mammalian nerve-muscle preparations have been described. 2 When ATX-II (10(-8)-10(-6) M) was applied to rat hemidiaphragm preparations, both directly and indirectly generated twitch responses were potentiated and prolonged. At the same time the resting tension of the preparations increased. 3 The increase in resting tension caused by ATX-II in innervated muscles was not prevented by curarization, but was reversed by exposure to tetrodotoxin. The increase in denervated muscles was not completely reversed by tetrodotoxin. 4 At concentrations exceeding 1 x 10(-7) M, ATX-II caused a sodium-dependent depolarization of both normal and denervated muscles. The depolarization of the denervated muscles was only partially reversed by tetrodotoxin. 5 In the presence of ATX-II repetitive endplate potentials (e.p.ps) were evoked by single shocks to the motor nerves in many fibres, and in those in which a single e.p.p. was still observed, the quantum content (m) was increased. Miniature e.p.p. frequency was not increased by ATX-II, even when muscle fibres were depolarized by 30 mV. 6 The indirectly and directly elicited action potentials of normal and denervated muscle fibres were much prolonged by ATX-II. The action potentials remained sodium-dependent. The sodium-dependent tetrodotoxin-resistant action potential of the denervated muscle fibre was also prolonged by ATX-II. 7 It is concluded that ATX-II both activates, and delays inactivation of, sodium channels in mammalian skeletal muscle fibres, probably in interacting with the channel "gate'. PMID:6115695

  19. Nuclear magnetic resonance study of the solution properties of the polypeptide neurotoxin I from the sea anemone Stichodactyla helianthus

    SciTech Connect

    Norton, R.S.; Cossins, A.I.; Kem, W.R. )

    1989-02-21

    The solution properties of the polypeptide neurotoxin I from the sea anemone Stichodactyla helianthus (Sh I) have been investigated by high-resolution H nuclear magnetic resonance (NMR) spectroscopy at 300 MHz. The pH dependence of the spectra has been examined over the range 1.1-12.2 at 27{degree}C. Individual pK{sub a} values have been obtained for the {alpha}-ammonium group of Ala-1 (8.6) and the side chains of Glu-8 (3.7), Tyr-36 (10.9), and Tyr-37 (10.8). For the remaining seven carboxyl groups in the molecule, four pK{sub a} values can be clearly identified. The five Lys residues titrate in the range 10.5-11, but individual pK{sub a} values could not be obtained because of peak overlap. Conformational changes associated with the protonation of carboxylates occur below pH 4, while in the alkaline pH range major unfolding occurs above pH 10. The molecule also unfolds at elevated temperatures. Exchange of the backbone amide protons has been monitored at various values of pH and temperature in the ranges pH 4-5 and 12-27{degree}C. Comparison of these properties of Sh I in solution with those of the related polypeptides anthopleurin A and Anemonia sulcata toxins I and II indicates that Sh I is less stable thermally and that there are some significant differences in the ionic interactions that maintain the tertiary structure. The solvent accessibility of aromatic residues has been probed with photochemically induced dynamic nuclear polarization NMR at 360 MHz.

  20. Neptunomonas phycophila sp. nov. isolated from a culture of Symbiodinium sp., a dinoflagellate symbiont of the sea anemone Aiptasia tagetes.

    PubMed

    Frommlet, Jörg; Guimarães, Bárbara; Sousa, Lígia; Serôdio, João; Alves, Artur

    2015-03-01

    A Gram-stain-negative, facultatively anaerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain SYM1(T), was isolated from a culture of Symbiodinium sp., an algal symbiont of the sea anemone Aiptasia tagetes collected in Puerto Rico. Growth was observed at 4-40 °C (optimum 30 °C), at pH 5.0-11.0 (optimum pH 8.0) and with 0.5-8?% (optimum 2?%) (w/v) NaCl. Phylogenetic analyses of 16S rRNA gene sequences showed that strain SYM1(T) was a member of the genus Neptunomonas with the type strain of Neptunomonas naphthovorans as the closest phylogenetic relative with a pairwise sequence similarity of 98.15?%. However, DNA-DNA relatedness between strain SYM1(T) and N. naphthovorans CIP 106451(T) was 24?%. Moreover, strain SYM1(T) could be distinguished from its closest relative by several phenotypic characteristics such as NaCl, pH and temperature tolerance, nitrate reduction and utilization of carbon substrates. The major cellular fatty acids were C16?:?0, C18?:?1?7c and summed feature 3 (comprising C16?:?1?7c and/or iso-C15?:?0 2-OH). The genomic DNA G+C content of strain SYM1(T) was 45 mol%. Ubiquinone-8 (Q-8) was the only respiratory quinone detected. Based on a polyphasic taxonomic characterization, strain SYM1(T) represents a novel species of the genus Neptunomonas, for which the name Neptunomonas phycophila sp. nov. is proposed. The type strain is SYM1(T) (?=?LMG 28329(T)?=?CECT 8716(T)). PMID:25563909

  1. Complex colony-level organization of the deep-sea siphonophore Bargmannia elongata (Cnidaria, Hydrozoa) is directionally asymmetric and arises by the subdivision of pro-buds.

    PubMed

    Dunn, Casey W

    2005-12-01

    Siphonophores are free-swimming colonial hydrozoans (Cnidaria) composed of asexually produced multicellular zooids. These zooids, which are homologous to solitary animals, are functionally specialized and arranged in complex species-specific patterns. The coloniality of siphonophores provides an opportunity to study the major transitions in evolution that give rise to new levels of biological organization, but siphonophores are poorly known because they are fragile and live in the open ocean. The organization and development of the deep-sea siphonophore Bargmannia elongata is described here using specimens collected with a remotely operated underwater vehicle. Each bud gives rise to a precise, directionally asymmetric sequence of zooids through a stereotypical series of subdivisions, rather than to a single zooid as in most other hydrozoans. This initial description of development in a deep-sea siphonophore provides an example of how precise colony-level organization can arise, and illustrates that the morphological complexity of cnidarians is greater than is often assumed. PMID:15986453

  2. Menthol-induced bleaching rapidly and effectively provides experimental aposymbiotic sea anemones (Aiptasia sp.) for symbiosis investigations.

    PubMed

    Matthews, Jennifer L; Sproles, Ashley E; Oakley, Clinton A; Grossman, Arthur R; Weis, Virginia M; Davy, Simon K

    2016-02-01

    Experimental manipulation of the symbiosis between cnidarians and photosynthetic dinoflagellates (Symbiodinium spp.) is crucial to advancing the understanding of the cellular mechanisms involved in host-symbiont interactions, and overall coral reef ecology. The anemone Aiptasia sp. is a model for cnidarian-dinoflagellate symbiosis, and notably it can be rendered aposymbiotic (i.e. dinoflagellate-free) and re-infected with a range of Symbiodinium types. Various methods exist for generating aposymbiotic hosts; however, they can be hugely time consuming and not wholly effective. Here, we optimise a method using menthol for production of aposymbiotic Aiptasia. The menthol treatment produced aposymbiotic hosts within just 4 weeks (97-100% symbiont loss), and the condition was maintained long after treatment when anemones were held under a standard light:dark cycle. The ability of Aiptasia to form a stable symbiosis appeared to be unaffected by menthol exposure, as demonstrated by successful re-establishment of the symbiosis when anemones were experimentally re-infected. Furthermore, there was no significant impact on photosynthetic or respiratory performance of re-infected anemones. PMID:26596538

  3. Reproduction in two deep-sea anemones (Actiniaria); Phelliactis hertwigi and P. robusta

    NASA Astrophysics Data System (ADS)

    van Praët, M.; Rice, A. L.; Thurston, M. H.

    Bathymetric distribution, abundance, substrate choice and gametogenesis have been investigated in two species of deep-sea actiniarians found in the northeast Atlantic Ocean. Phelliactis hertwigi occurs at 719-1448m in the Porcupine Seabight, usually encloses a bolus of sediment within its highly concave pedal disc (79.7%), and has abundances of up to 14.5 individuals 1000m -2. Phelliactis robusta occurs at 1600-2173m in the Porcupine Seabight, but extends deeper in the Bay of Biscay. In areas of soft sediment it is associated strongly with clinker (92.7%) and attains densities of 2.9 individuals 1000m -2. Both species are dioecious. Their sperm appear similar to those of the related intertidal Calliactis spp. Previtellogenesis and vitellogenesis of the oocytes have been defined by ultrastructural and histochemical studies. In P. hertwigi mature oocytes measure up to 180μm, and to 210μm in P. robusta. In P. hertwigi oogenesis takes 8-9 months, with spawning in October/November, whereas in the deeper-living P. robusta oogenesis occupies 15 to 19 months and spawning occurs in April/ May. Evidence is produced to suggest that these two contrasting cycles are related to the rate and seasonality of deposition of organic matter to the deep-sea floor.

  4. Development of highly selective Kv1.3-blocking peptides based on the sea anemone peptide ShK.

    PubMed

    Pennington, Michael W; Chang, Shih Chieh; Chauhan, Satendra; Huq, Redwan; Tajhya, Rajeev B; Chhabra, Sandeep; Norton, Raymond S; Beeton, Christine

    2015-01-01

    ShK, from the sea anemone Stichodactyla helianthus, is a 35-residue disulfide-rich peptide that blocks the voltage-gated potassium channel Kv1.3 at ca. 10 pM and the related channel Kv1.1 at ca. 16 pM. We developed an analog of this peptide, ShK-186, which is currently in Phase 1b-2a clinical trials for the treatment of autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. While ShK-186 displays a >100-fold improvement in selectivity for Kv1.3 over Kv1.1 compared with ShK, there is considerable interest in developing peptides with an even greater selectivity ratio. In this report, we describe several variants of ShK that incorporate p-phophono-phenylalanine at the N-terminus coupled with internal substitutions at Gln16 and Met21. In addition, we also explored the combinatorial effects of these internal substitutions with an alanine extension at the C-terminus. Their selectivity was determined by patch-clamp electrophysiology on Kv1.3 and Kv1.1 channels stably expressed in mouse fibroblasts. The peptides with an alanine extension blocked Kv1.3 at low pM concentrations and exhibited up to 2250-fold selectivity for Kv1.3 over Kv1.1. Analogs that incorporates p-phosphono-phenylalanine at the N-terminus blocked Kv1.3 with IC50s in the low pM range and did not affect Kv1.1 at concentrations up to 100 nM, displaying a selectivity enhancement of >10,000-fold for Kv1.3 over Kv1.1. Other potentially important Kv channels such as Kv1.4 and Kv1.6 were only partially blocked at 100 nM concentrations of each of the ShK analogs. PMID:25603346

  5. Structure-function relationships of the major neurotoxin from the sea anemone Stichodactyla helianthus with a new sodium channel receptor site

    SciTech Connect

    Pennington, M.W.

    1988-01-01

    We have determined that ShN I, a 48-residue type 2 sea anemone toxin, delays the inactivation of the Na channel in lobster olfactory somas. The receptor for ShN I was identified in vesicle preparations of neuronal tissues from both crustaceans and mammals; however, the K{sub D} values for the former is more than 1,000 fold lower for the later. The binding of ({sup 125}I)-ShN I to this receptor was determined to be unaffected by Anemonia sulcata II, depolarization of the membrane, or veratridine. ShN I was unable to displace ({sup 125}I)-Androctonus austrialis Hector II, whereas unlabeled AaH II and As II displaced the labeled scorpion toxin from rat brain synaptosomes. This is the first characterization of a new Na channel receptor site which specifically binds type 2 anemone toxins. To study the interactions that specific amino acid residues of ShN I have with this receptor, we developed a strategy using solid phase peptide synthesis. Prior to the synthesis of analogs to ShN I, we assembled the native ShN I sequence and reoxidized the three intramolecular disulfide bonds. Chemical, physical, and pharmacological characterization of the purified synthetic ShN I showed it to be indistinguishable from the natural toxin.

  6. The sea anemone Bunodosoma caissarum toxin BcIII modulates the sodium current kinetics of rat dorsal root ganglia neurons and is displaced in a voltage-dependent manner.

    PubMed

    Salceda, Emilio; López, Omar; Zaharenko, André J; Garateix, Anoland; Soto, Enrique

    2010-03-01

    Sea anemone toxins bind to site 3 of the sodium channels, which is partially formed by the extracellular linker connecting S3 and S4 segments of domain IV, slowing down the inactivation process. In this work we have characterized the actions of BcIII, a sea anemone polypeptide toxin isolated from Bunodosoma caissarum, on neuronal sodium currents using the patch clamp technique. Neurons of the dorsal root ganglia of Wistar rats (P5-9) in primary culture were used for this study (n=65). The main effects of BcIII were a concentration-dependent increase in the sodium current inactivation time course (IC(50)=2.8 microM) as well as an increase in the current peak amplitude. BcIII did not modify the voltage at which 50% of the channels are activated or inactivated, nor the reversal potential of sodium current. BcIII shows a voltage-dependent action. A progressive acceleration of sodium current fast inactivation with longer conditioning pulses was observed, which was steeper as more depolarizing were the prepulses. The same was observed for other two anemone toxins (CgNa, from Condylactis gigantea and ATX-II, from Anemonia viridis). These results suggest that the binding affinity of sea anemone toxins may be reduced in a voltage-dependent manner, as has been described for alpha-scorpion toxins. PMID:20015459

  7. Detrimental effects of host anemone bleaching on anemonefish populations

    NASA Astrophysics Data System (ADS)

    Saenz-Agudelo, P.; Jones, G. P.; Thorrold, S. R.; Planes, S.

    2011-06-01

    Coral bleaching and related reef degradation have caused significant declines in the abundance of reef-associated fishes. Most attention on the effects of bleaching has focused on corals, but bleaching is also prevalent in other cnidarians, including sea anemones. The consequences of anemone bleaching are unknown, and the demographic effects of bleaching on associated fish recruitment, survival, and reproduction are poorly understood. We examined the effect of habitat degradation including host anemone bleaching on fish abundance, egg production, and recruitment of the panda anemonefish ( Amphiprion polymnus) near Port Moresby, Papua New Guinea. Following a high-temperature anomaly in shallow waters of the region, most shallow anemones to a depth of 6 m (approximately 35% of all the anemones in this area) were severely bleached. Anemone mortality was low but bleached anemones underwent a ~34% reduction in body size. Total numbers of A. polymnus were not affected by bleaching and reduction in shelter area. While egg production of females living in bleached anemones was reduced by ~38% in 2009 compared to 2008, egg production of females on unbleached anemones did not differ significantly between years. Total recruitment in 2009 was much lower than in 2008. However, we found no evidence of recruiting larvae avoiding bleached anemones at settlement suggesting that other factors or different chemical cues were more important in determining recruitment than habitat quality. These results provide the first field evidence of detrimental effects of climate-induced bleaching and habitat degradation on reproduction and recruitment of anemonefish.

  8. Molecular analysis of the sea anemone toxin Av3 reveals selectivity to insects and demonstrates the heterogeneity of receptor site-3 on voltage-gated Na+ channels.

    PubMed

    Moran, Yehu; Kahn, Roy; Cohen, Lior; Gur, Maya; Karbat, Izhar; Gordon, Dalia; Gurevitz, Michael

    2007-08-15

    Av3 is a short peptide toxin from the sea anemone Anemonia viridis shown to be active on crustaceans and inactive on mammals. It inhibits inactivation of Na(v)s (voltage-gated Na+ channels) like the structurally dissimilar scorpion alpha-toxins and type I sea anemone toxins that bind to receptor site-3. To examine the potency and mode of interaction of Av3 with insect Na(v)s, we established a system for its expression, mutagenized it throughout, and analysed it in toxicity, binding and electrophysiological assays. The recombinant Av3 was found to be highly toxic to blowfly larvae (ED50=2.65+/-0.46 pmol/100 mg), to compete well with the site-3 toxin LqhalphaIT (from the scorpion Leiurus quinquestriatus) on binding to cockroach neuronal membranes (K(i)=21.4+/-7.1 nM), and to inhibit the inactivation of Drosophila melanogaster channel, DmNa(v)1, but not that of mammalian Na(v)s expressed in Xenopus oocytes. Moreover, like other site-3 toxins, the activity of Av3 was synergically enhanced by ligands of receptor site-4 (e.g. scorpion beta-toxins). The bioactive surface of Av3 was found to consist mainly of aromatic residues and did not resemble any of the bioactive surfaces of other site-3 toxins. These analyses have portrayed a toxin that might interact with receptor site-3 in a different fashion compared with other ligands of this site. This assumption was corroborated by a D1701R mutation in DmNa(v)1, which has been shown to abolish the activity of all other site-3 ligands, except Av3. All in all, the present study provides further evidence for the heterogeneity of receptor site-3, and raises Av3 as a unique model for design of selective anti-insect compounds. PMID:17492942

  9. Increasing pCO2 correlates with low concentrations of intracellular dimethylsulfoniopropionate in the sea anemone Anemonia viridis.

    PubMed

    Borell, Esther M; Steinke, Michael; Horwitz, Rael; Fine, Maoz

    2014-02-01

    Marine anthozoans maintain a mutualistic symbiosis with dinoflagellates that are prolific producers of the algal secondary metabolite dimethylsulfoniopropionate (DMSP), the precursor of the climate-cooling trace gas dimethyl sulfide (DMS). Surprisingly, little is known about the physiological role of DMSP in anthozoans and the environmental factors that regulate its production. Here, we assessed the potential functional role of DMSP as an antioxidant and determined how future increases in seawater pCO2 may affect DMSP concentrations in the anemone Anemonia viridis along a natural pCO2 gradient at the island of Vulcano, Italy. There was no significant difference in zooxanthellae genotype and characteristics (density of zooxanthellae, and chlorophyll a) as well as protein concentrations between anemones from three stations along the gradient, V1 (3232 ?atm CO2), V2 (682 ?atm) and control (463 ?atm), which indicated that A.?viridis can acclimate to various seawater pCO2. In contrast, DMSP concentrations in anemones from stations V1 (33.23?±?8.30 fmol cell(-1)) and V2 (34.78?±?8.69 fmol cell(-1)) were about 35% lower than concentrations in tentacles from the control station (51.85?±?12.96 fmol cell(-1)). Furthermore, low tissue concentrations of DMSP coincided with low activities of the antioxidant enzyme superoxide dismutase (SOD). Superoxide dismutase activity for both host (7.84?±?1.37?U·mg(-1) protein) and zooxanthellae (2.84?±?0.41?U·mg(-1) protein) at V1 was 40% lower than at the control station (host: 13.19?±?1.42; zooxanthellae: 4.72?±?0.57?U·mg(-1) protein). Our results provide insight into coastal DMSP production under predicted environmental change and support the function of DMSP as an antioxidant in symbiotic anthozoans. PMID:24634728

  10. Increasing pCO2 correlates with low concentrations of intracellular dimethylsulfoniopropionate in the sea anemone Anemonia viridis

    PubMed Central

    Borell, Esther M; Steinke, Michael; Horwitz, Rael; Fine, Maoz

    2014-01-01

    Marine anthozoans maintain a mutualistic symbiosis with dinoflagellates that are prolific producers of the algal secondary metabolite dimethylsulfoniopropionate (DMSP), the precursor of the climate-cooling trace gas dimethyl sulfide (DMS). Surprisingly, little is known about the physiological role of DMSP in anthozoans and the environmental factors that regulate its production. Here, we assessed the potential functional role of DMSP as an antioxidant and determined how future increases in seawater pCO2 may affect DMSP concentrations in the anemone Anemonia viridis along a natural pCO2 gradient at the island of Vulcano, Italy. There was no significant difference in zooxanthellae genotype and characteristics (density of zooxanthellae, and chlorophyll a) as well as protein concentrations between anemones from three stations along the gradient, V1 (3232 ?atm CO2), V2 (682 ?atm) and control (463 ?atm), which indicated that A.?viridis can acclimate to various seawater pCO2. In contrast, DMSP concentrations in anemones from stations V1 (33.23?±?8.30 fmol cell?1) and V2 (34.78?±?8.69 fmol cell?1) were about 35% lower than concentrations in tentacles from the control station (51.85?±?12.96 fmol cell?1). Furthermore, low tissue concentrations of DMSP coincided with low activities of the antioxidant enzyme superoxide dismutase (SOD). Superoxide dismutase activity for both host (7.84?±?1.37?U·mg?1 protein) and zooxanthellae (2.84?±?0.41?U·mg?1 protein) at V1 was 40% lower than at the control station (host: 13.19?±?1.42; zooxanthellae: 4.72?±?0.57?U·mg?1 protein). Our results provide insight into coastal DMSP production under predicted environmental change and support the function of DMSP as an antioxidant in symbiotic anthozoans. PMID:24634728

  11. CgNa, a type I toxin from the giant Caribbean sea anemone Condylactis gigantea shows structural similarities to both type I and II toxins, as well as distinctive structural and functional properties1

    PubMed Central

    Salceda, Emilio; Pérez-Castells, Javier; López-Méndez, Blanca; Garateix, Anoland; Salazar, Hector; López, Omar; Aneiros, Abel; Ständker, Ludger; Béress, Lászlo; Forssmann, Wolf-Georg; Soto, Enrique; Jiménez-Barbero, Jesús; Giménez-Gallego, Guillermo

    2007-01-01

    CgNa (Condylactis gigantea neurotoxin) is a 47-amino-acid- residue toxin from the giant Caribbean sea anemone Condylactis gigantea. The structure of CgNa, which was solved by 1H-NMR spectroscopy, is somewhat atypical and displays significant homology with both type I and II anemone toxins. CgNa also displays a considerable number of exceptions to the canonical structural elements that are thought to be essential for the activity of this group of toxins. Furthermore, unique residues in CgNa define a characteristic structure with strong negatively charged surface patches. These patches disrupt a surface-exposed cluster of hydrophobic residues present in all anemone-derived toxins described to date. A thorough characterization by patch–clamp analysis using rat DRG (dorsal root ganglion) neurons indicated that CgNa preferentially binds to TTX-S (tetrodotoxin-sensitive) voltage-gated sodium channels in the resting state. This association increased the inactivation time constant and the rate of recovery from inactivation, inducing a significant shift in the steady state of inactivation curve to the left. The specific structural features of CgNa may explain its weaker inhibitory capacity when compared with the other type I and II anemone toxins. PMID:17506725

  12. The effect of cholesterol on the long-range network of interactions established among sea anemone Sticholysin II residues at the water-membrane interface.

    PubMed

    García-Linares, Sara; Alm, Ida; Maula, Terhi; Gavilanes, José G; Slotte, Johan Peter; Martínez-Del-Pozo, Álvaro

    2015-04-01

    Actinoporins are ?-pore forming proteins with therapeutic potential, produced by sea anemones. Sticholysin II (StnII) from Stichodactyla helianthus is one of its most extensively characterized members. These proteins remain stably folded in water, but upon interaction with lipid bilayers, they oligomerize to form a pore. This event is triggered by the presence of sphingomyelin (SM), but cholesterol (Chol) facilitates pore formation. Membrane attachment and pore formation require changes involving long-distance rearrangements of residues located at the protein-membrane interface. The influence of Chol on membrane recognition, oligomerization, and/or pore formation is now studied using StnII variants, which are characterized in terms of their ability to interact with model membranes in the presence or absence of Chol. The results obtained frame Chol not only as an important partner for SM for functional membrane recognition but also as a molecule which significantly reduces the structural requirements for the mentioned conformational rearrangements to occur. However, given that the DOPC:SM:Chol vesicles employed display phase coexistence and have domain boundaries, the observed effects could be also due to the presence of these different phases on the membrane. In addition, it is also shown that the Arg51 guanidinium group is strictly required for membrane recognition, independently of the presence of Chol. PMID:25815890

  13. Effects of toxin isolated from the sea anemone Bolocera tuediae on electrical properties of isolated rat skeletal muscle and cultured myotubes.

    PubMed

    Tesseraux, I; Gülden, M; Schumann, G

    1989-01-01

    Electrophysiological effects of a polypeptide toxin isolated from the sea anemone Bolocera tuediae (BTTX II) on isolated fast and slow rat skeletal muscle and on cultured rat myotubes are described in this paper. Nanomolar concentrations of BTTX II cause a concentration-dependent depolarization. This effect is prevented by the presence of tetrodotoxin. Action potential duration is prolonged in all preparations investigated, with the fast muscle being the least sensitive. The positive overshoot and the maximum rate of rise of the action potential is decreased by BTTX II. Cultured myotubes which are less susceptible to the sodium channel blocking activity of tetrodotoxin have the highest sensitivity to all actions of BTTX II. It is further shown that the proportion of spontaneously contracting myotubes is diminished and the frequency of contractions is increased by the toxin. The effects of Bolocera tuediae toxin II resemble those observed with Anemonia sulcata toxin II on mammalian skeletal muscle. From the results it is concluded, that BTTX II may interfere with both the activation and inactivation of sodium channels of the muscle membrane. PMID:2566207

  14. Nitric oxide mediates coral bleaching through an apoptotic-like cell death pathway: evidence from a model sea anemone-dinoflagellate symbiosis.

    PubMed

    Hawkins, Thomas D; Bradley, Benjamin J; Davy, Simon K

    2013-12-01

    Coral bleaching (involving the loss of symbiotic algae from the cnidarian host) is a major threat to coral reefs and appears to be mediated at the cellular level by nitric oxide (NO). In this study, we examined the specific role of NO in bleaching using the sea anemone Aiptasia pulchella, a model system for the study of corals. Exposure of A. pulchella to high-temperature shock (26-33°C over <1 h) or an NO donor (S-nitrosoglutathione) resulted in significant increases in host caspase-like enzyme activity. These responses were reflected in the intensities of bleaching, which were significantly higher in heat- or NO-treated specimens than in controls maintained in seawater at 26°C. Notably, the inhibition of caspase-like activity prevented bleaching even in the presence of an NO donor or at elevated temperature. The additional use of an NO scavenger controlled for effects mediated by agents other than NO. We also exposed A. pulchella to a more ecologically relevant treatment (an increase from 26 to 33°C over 6-7 d). Again, host NO synthesis correlated with the activation of caspase-like enzyme activity. Therefore, we conclude that NO's involvement in cnidarian bleaching arises through the regulation of host apoptotic pathways. PMID:23934282

  15. A Protein Toxin from the Sea Anemone Phyllodiscus semoni Targets the Kidney and Causes a Severe Renal Injury with Predominant Glomerular Endothelial Damage

    PubMed Central

    Mizuno, Masashi; Nozaki, Masatoshi; Morine, Nobuya; Suzuki, Norihiko; Nishikawa, Kazuhiro; Morgan, B. Paul; Matsuo, Seiichi

    2007-01-01

    Envenomation by the sea anemone Phyllodiscus semoni causes fulminant dermatitis and, rarely, acute renal failure in humans. Here, we investigated whether the venom extracted from the nematocysts (PsTX-T) was nephrotoxic when administered intravenously in rats and whether PsTX-T induced activation of the complement system. Although small dose of PsTX-T induced acute tubular necrosis in rats resembling pathology seen in patients, kidneys displayed glomerular injury with glomerular endothelial damage, thrombus formation, mesangiolysis, and partial rupture of glomerular basement membrane, accompanied by severe tubular necrosis at 24 hours after administration of 0.03 mg of PsTX-T per animal, similar to the glomerular findings typical of severe hemolytic uremic syndrome. The early stage injury was accompanied by specific PsTX-T binding, massive complement C3b, and membrane attack complex deposition in glomeruli in the regions of injury and decreased glomerular expression of complement regulators. A pathogenic role for complement was confirmed by demonstrating that systemic complement inhibition reduced renal injury. The isolated nephrotoxic component, a 115-kd protein toxin (PsTX-115), was shown to cause identical renal pathology. The demonstration that PsTX-T and PsTX-115 were highly nephrotoxic acting via induction of complement activation suggests that inhibition of complement might be used to prevent acute renal damage following envenomation by P. semoni. PMID:17600120

  16. Improved purification and enzymatic properties of a mixture of Sticholysin I and II: isotoxins with hemolytic and phospholipase A(2) activities from the sea anemone Stichodactyla helianthus.

    PubMed

    del Monte-Martínez, Alberto; González-Bacerio, Jorge; Romero, Lázara; Aragón, Carlos; Martínez, Diana; de Los Á Chávez, María; Álvarez, Carlos; Lanio, María E; Guisán, José M; Díaz, Joaquín

    2014-03-01

    Sticholysin I and Sticholysin II (StI and StII) are two potent hemolysins which form pores in natural and model membranes at nanomolar concentrations. These proteins were purified from the aqueous extract of the sea anemone Stichodactyla helianthus, Ellis 1768, by gel filtration and ionic exchange chromatography. This procedure rendered StI and StII with high purity (purification factors: 36 and 50, respectively) but a low yield of hemolytic activity, HA (<3%). Additionally, these toxins exhibited very low phospholipase activity (10(-3)U/mg of protein). In this work, a mixture StI-StII was obtained (yield >95%, with an increase in specific activity: 14 times) from the animal extract using an oxidized phospholipid-based affinity chromatographic matrix binding phospholipases. Cytolysin identification in the mixture was performed by immunoblotting and N-terminal sequence analyses. Phospholipase A2 (PLA2) activity of StI-StII was relatively high (1.85U/mg) and dependent of Ca(2+). The activity resulted optimum when was measured with the mostly unsaturated soybean phosphatidylcholine (PC), when compared to the less unsaturated egg PC or completely saturated dipalmitoyl PC, in the presence of 40mM Ca(2+) at pH 8.0. This Ca(2+) concentration did not exert any effect on binding of StI-StII with soybean PC monolayers. Then, PLA2 activity seems not be required to binding to membranes. PMID:24326193

  17. The Effect of Cholesterol on the Long-Range Network of Interactions Established among Sea Anemone Sticholysin II Residues at the Water-Membrane Interface

    PubMed Central

    García-Linares, Sara; Alm, Ida; Maula, Terhi; Gavilanes, José G.; Slotte, Johan Peter; Martínez-del-Pozo, Álvaro

    2015-01-01

    Actinoporins are ?-pore forming proteins with therapeutic potential, produced by sea anemones. Sticholysin II (StnII) from Stichodactyla helianthus is one of its most extensively characterized members. These proteins remain stably folded in water, but upon interaction with lipid bilayers, they oligomerize to form a pore. This event is triggered by the presence of sphingomyelin (SM), but cholesterol (Chol) facilitates pore formation. Membrane attachment and pore formation require changes involving long-distance rearrangements of residues located at the protein-membrane interface. The influence of Chol on membrane recognition, oligomerization, and/or pore formation is now studied using StnII variants, which are characterized in terms of their ability to interact with model membranes in the presence or absence of Chol. The results obtained frame Chol not only as an important partner for SM for functional membrane recognition but also as a molecule which significantly reduces the structural requirements for the mentioned conformational rearrangements to occur. However, given that the DOPC:SM:Chol vesicles employed display phase coexistence and have domain boundaries, the observed effects could be also due to the presence of these different phases on the membrane. In addition, it is also shown that the Arg51 guanidinium group is strictly required for membrane recognition, independently of the presence of Chol. PMID:25815890

  18. Generation and analysis of transcriptomic resources for a model system on the rise: the sea anemone Aiptasia pallida and its dinoflagellate endosymbiont

    PubMed Central

    Sunagawa, Shinichi; Wilson, Emily C; Thaler, Michael; Smith, Marc L; Caruso, Carlo; Pringle, John R; Weis, Virginia M; Medina, Mónica; Schwarz, Jodi A

    2009-01-01

    Background The most diverse marine ecosystems, coral reefs, depend upon a functional symbiosis between cnidarian hosts and unicellular dinoflagellate algae. The molecular mechanisms underlying the establishment, maintenance, and breakdown of the symbiotic partnership are, however, not well understood. Efforts to dissect these questions have been slow, as corals are notoriously difficult to work with. In order to expedite this field of research, we generated and analyzed a collection of expressed sequence tags (ESTs) from the sea anemone Aiptasia pallida and its dinoflagellate symbiont (Symbiodinium sp.), a system that is gaining popularity as a model to study cellular, molecular, and genomic questions related to cnidarian-dinoflagellate symbioses. Results A set of 4,925 unique sequences (UniSeqs) comprising 1,427 clusters of 2 or more ESTs (contigs) and 3,498 unclustered ESTs (singletons) was generated by analyzing 10,285 high-quality ESTs from a mixed host/symbiont cDNA library. Using a BLAST-based approach to predict which unique sequences derived from the host versus symbiont genomes, we found that the contribution of the symbiont genome to the transcriptome was surprisingly small (1.6–6.4%). This may reflect low levels of gene expression in the symbionts, low coverage of alveolate genes in the sequence databases, a small number of symbiont cells relative to the total cellular content of the anemones, or failure to adequately lyse symbiont cells. Furthermore, we were able to identify groups of genes that are known or likely to play a role in cnidarian-dinoflagellate symbioses, including oxidative stress pathways that emerged as a prominent biological feature of this transcriptome. All ESTs and UniSeqs along with annotation results and other tools have been made accessible through the implementation of a publicly accessible database named AiptasiaBase. Conclusion We have established the first large-scale transcriptomic resource for Aiptasia pallida and its dinoflagellate symbiont. These data provide researchers with tools to study questions related to cnidarian-dinoflagellate symbioses on a molecular, cellular, and genomic level. This groundwork represents a crucial step towards the establishment of a tractable model system that can be utilized to better understand cnidarian-dinoflagellate symbioses. With the advent of next-generation sequencing methods, the transcriptomic inventory of A. pallida and its symbiont, and thus the extent of AiptasiaBase, should expand dramatically in the near future. PMID:19500365

  19. Production of a reference transcriptome and transcriptomic database (EdwardsiellaBase) for the lined sea anemone, Edwardsiella lineata, a parasitic cnidarian

    PubMed Central

    2014-01-01

    Background The lined sea anemone Edwardsiella lineata is an informative model system for evolutionary-developmental studies of parasitism. In this species, it is possible to compare alternate developmental pathways leading from a larva to either a free-living polyp or a vermiform parasite that inhabits the mesoglea of a ctenophore host. Additionally, E. lineata is confamilial with the model cnidarian Nematostella vectensis, providing an opportunity for comparative genomic, molecular and organismal studies. Description We generated a reference transcriptome for E. lineata via high-throughput sequencing of RNA isolated from five developmental stages (parasite; parasite-to-larva transition; larva; larva-to-adult transition; adult). The transcriptome comprises 90,440 contigs assembled from >15 billion nucleotides of DNA sequence. Using a molecular clock approach, we estimated the divergence between E. lineata and N. vectensis at 215–364 million years ago. Based on gene ontology and metabolic pathway analyses and gene family surveys (bHLH-PAS, deiodinases, Fox genes, LIM homeodomains, minicollagens, nuclear receptors, Sox genes, and Wnts), the transcriptome of E. lineata is comparable in depth and completeness to N. vectensis. Analyses of protein motifs and revealed extensive conservation between the proteins of these two edwardsiid anemones, although we show the NF-?B protein of E. lineata reflects the ancestral structure, while the NF-?B protein of N. vectensis has undergone a split that separates the DNA-binding domain from the inhibitory domain. All contigs have been deposited in a public database (EdwardsiellaBase), where they may be searched according to contig ID, gene ontology, protein family motif (Pfam), enzyme commission number, and BLAST. The alignment of the raw reads to the contigs can also be visualized via JBrowse. Conclusions The transcriptomic data and database described here provide a platform for studying the evolutionary developmental genomics of a derived parasitic life cycle. In addition, these data from E. lineata will aid in the interpretation of evolutionary novelties in gene sequence or structure that have been reported for the model cnidarian N. vectensis (e.g., the split NF-?B locus). Finally, we include custom computational tools to facilitate the annotation of a transcriptome based on high-throughput sequencing data obtained from a “non-model system.” PMID:24467778

  20. Giant Caribbean Anemone (Condylactis gigantea)

    USGS Multimedia Gallery

    Condylactis gigantea fluourescing on rocks. The anemones are in a tank illuminated by ultraviolet (black) light. The anemones absorb the invisible ultraviolet light and reflect it back as visible bright green light....

  1. Cyphastrea kausti sp. n. (Cnidaria, Anthozoa, Scleractinia), a new species of reef coral from the Red Sea

    PubMed Central

    Bouwmeester, Jessica; Benzoni, Francesca; Baird, Andrew H.; Berumen, Michael L.

    2015-01-01

    Abstract A new scleractinian coral species, Cyphastrea kausti sp. n., is described from 13 specimens from the Red Sea. It is characterised by the presence of eight primary septa, unlike the other species of the genus, which have six, ten or 12 primary septa. The new species has morphological affinities with Cyphastrea microphthalma, from which it can be distinguished by the lower number of septa (on average eight instead of ten), and smaller calices and corallites. This species was observed in the northern and central Red Sea and appears to be absent from the southern Red Sea. PMID:25931952

  2. The specificity of Av3 sea anemone toxin for arthropods is determined at linker DI/SS2-S6 in the pore module of target sodium channels.

    PubMed

    Gur Barzilai, Maya; Kahn, Roy; Regev, Noa; Gordon, Dalia; Moran, Yehu; Gurevitz, Michael

    2014-10-15

    Av3 is a peptide neurotoxin from the sea anemone Anemonia viridis that shows specificity for arthropod voltage-gated sodium channels (Navs). Interestingly, Av3 competes with a scorpion ?-toxin on binding to insect Navs and similarly inhibits the inactivation process, and thus has been classified as 'receptor site-3 toxin', although the two peptides are structurally unrelated. This raises questions as to commonalities and differences in the way both toxins interact with Navs. Recently, site-3 was partly resolved for scorpion ?-toxins highlighting S1-S2 and S3-S4 external linkers at the DIV voltage-sensor module and the juxtaposed external linkers at the DI pore module. To uncover channel determinants involved in Av3 specificity for arthropods, the toxin was examined on channel chimaeras constructed with the external linkers of the mammalian brain Nav1.2a, which is insensitive to Av3, in the background of the Drosophila DmNav1. This approach highlighted the role of linker DI/SS2-S6, adjacent to the channel pore, in determining Av3 specificity. Point mutagenesis at DI/SS2-S6 accompanied by functional assays highlighted Trp404 and His405 as a putative point of Av3 interaction with DmNav1. His405 conservation in arthropod Navs compared with tyrosine in vertebrate Navs may represent an ancient substitution that explains the contemporary selectivity of Av3. Trp404 and His405 localization near the membrane surface and the hydrophobic bioactive surface of Av3 suggest that the toxin possibly binds at a cleft by DI/S6. A partial overlap in receptor site-3 of both toxins nearby DI/S6 may explain their binding competition capabilities. PMID:25055135

  3. Phyla- and Subtype-Selectivity of CgNa, a Na+ Channel Toxin from the Venom of the Giant Caribbean Sea Anemone Condylactis Gigantea

    PubMed Central

    Billen, Bert; Debaveye, Sarah; Béress, Lászlo; Tytgat, Jan

    2010-01-01

    Because of their prominent role in electro-excitability, voltage-gated sodium (NaV) channels have become the foremost important target of animal toxins. These toxins have developed the ability to discriminate between closely related NaV subtypes, making them powerful tools to study NaV channel function and structure. CgNa is a 47-amino acid residue type I toxin isolated from the venom of the Giant Caribbean Sea Anemone Condylactis gigantea. Previous studies showed that this toxin slows the fast inactivation of tetrodotoxin-sensitive NaV currents in rat dorsal root ganglion neurons. To illuminate the underlying NaV subtype-selectivity pattern, we have assayed the effects of CgNa on a broad range of mammalian isoforms (NaV1.2–NaV1.8) expressed in Xenopus oocytes. This study demonstrates that CgNa selectively slows the fast inactivation of rNaV1.3/?1, mNaV1.6/?1 and, to a lesser extent, hNaV1.5/?1, while the other mammalian isoforms remain unaffected. Importantly, CgNa was also examined on the insect sodium channel DmNaV1/tipE, revealing a clear phyla-selectivity in the efficacious actions of the toxin. CgNa strongly inhibits the inactivation of the insect NaV channel, resulting in a dramatic increase in peak current amplitude and complete removal of fast and steady-state inactivation. Together with the previously determined solution structure, the subtype-selective effects revealed in this study make of CgNa an interesting pharmacological probe to investigate the functional role of specific NaV channel subtypes. Moreover, further structural studies could provide important information on the molecular mechanism of NaV channel inactivation. PMID:21833172

  4. Cooperativity in the two-domain arginine kinase from the sea anemone Anthopleura japonicus. II. Evidence from site-directed mutagenesis studies.

    PubMed

    Tada, Hiroshi; Suzuki, Tomohiko

    2010-08-01

    The arginine kinase (AK) from the sea anemone Anthopleura japonicus has an unusual two-domain structure (contiguous dimer; denoted by D1-D2). In a previous report, we suggested cooperativity in the contiguous dimer, which may be a result of domain-domain interactions, using MBP-fused enzymes. To further understand this observation, we inserted six-Lys residues into the linker region of the two-domain AK (D1-K6-D2 mutant) using His-tagged enzyme. The dissociation constants, K(a) and K(ia), of the mutant were similar to those of the wild-type enzyme but the catalytic constant, k(cat), was decreased to 28% that of the wild-type, indicating that some of the domain-domain interactions are lost due to the six-Lys insertion. Y68 plays a major role in arginine binding in the catalytic pocket in Limulus AK, and introduction of mutation at the Y68 position virtually abolishes catalytic activity. Thus, the constructed D1(Y68G)-D2 and D1-D2(Y68G) mutants mimic the D1(inactive)-D2(active) and D1(active)-D2(inactive) enzymes, respectively. The k(cat) values of both Y68 mutants were decreased to 13-18% that of the wild-type enzyme, which is much less than the 50% level of the two-domain enzyme. Thus, it is clear that substrate-binding to both domains is necessary for full expression of activity. In other words, substrate-binding appears to act as the trigger of the functional cooperativity in two-domain AK. PMID:20434482

  5. A study on the membrane depolarization of skeletal muscles caused by a scorpion toxin, sea anemone toxin II and crotamine and the interaction between toxins.

    PubMed Central

    Chang, C. C.; Hong, S. J.; Su, M. J.

    1983-01-01

    Quinquestriatus toxin (QTX) isolated from the venom of a scorpion (Leiurus quinquestriatus) and sea anemone (Anemonia sulcata) toxin II enhanced the twitch response of the rat and mouse diaphragms and like crotamine (isolated from the venom of Crotalus durissus terrificus) caused spontaneous fasciculation of the muscle. Trains of action potentials in muscles at 70-250 Hz, which could not be antagonized by (+)-tubocurarine, were triggered by single stimulation or occurred spontaneously after treatment with these toxins. QTX and toxin II prolonged the rat muscle action potential 3 to 4 fold whereas crotamine prolonged the action potential by only 30%. The membrane potential was depolarized from about -82 mV to -55 mV by crotamine 2 micrograms ml-1, -41 mV by toxin II 5 micrograms ml-1 and to -50 mV by QTX 1 microgram ml-1. The concentrations to induce 50% maximal depolarization (K0.5) were 0.07, 0.15 and greater than 0.4 microgram ml-1, respectively, for QTX, crotamine and toxin II, whereas the rates of depolarization were in the order toxin II greater than or equal to crotamine greater than QTX. The depolarizing effects of crotamine and QTX, but not of toxin II, were saturable. The depolarizing effects of all three toxins were irreversible whereas the membrane potential could be restored by tetrodotoxin non-competitively. Simultaneous treatment with crotamine and QTX or crotamine and toxin II at concentrations below K0.5 caused only additive effects on depolarization. When the muscle was depolarized by pretreating with a saturating concentration of crotamine, the onset of depolarization by QTX was greatly retarded whereas that by toxin II was unaffected.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:6197125

  6. Pachyseris inattesa sp. n. (Cnidaria, Anthozoa, Scleractinia): a new reef coral species from the Red Sea and its phylogenetic relationships

    PubMed Central

    Terraneo, Tullia I.; Berumen, Michael L.; Arrigoni, Roberto; Waheed, Zarinah; Bouwmeester, Jessica; Caragnano, Annalisa; Stefani, Fabrizio; Benzoni, Francesca

    2014-01-01

    Abstract A new scleractinian coral species, Pachyseris inattesa sp. n., is described from the Red Sea. Despite a superficial resemblance with some species in the agariciid genus Leptoseris with which it has been previously confused, P. inattesa sp. n. has micro-morphological characters typical of the genus Pachyseris. This genus, once part of the Agariciidae, is comprised of five extant species and is widely distributed throughout the tropical Indo-Pacific. It is currently incertae sedis as a result of recent molecular analysis and appears to be closely related to the Euphylliidae. A molecular phylogenetic reconstruction including P. inattesa sp. n., the genus type species P. rugosa, and P. speciosa, all present in the Red Sea, was performed using the mitochondrial intergenic spacer between COI and 16S-rRNA. The results confirm that P. inattesa sp. n. is a monophyletic lineage closely related to the other Pachyseris species examined. PMID:25152672

  7. Two Alleles of NF-κB in the Sea Anemone Nematostella vectensis Are Widely Dispersed in Nature and Encode Proteins with Distinct Activities

    PubMed Central

    Sullivan, James C.; Wolenski, Francis S.; Reitzel, Adam M.; French, Courtney E.; Traylor-Knowles, Nikki; Gilmore, Thomas D.; Finnerty, John R.

    2009-01-01

    Background NF-κB is an evolutionarily conserved transcription factor that controls the expression of genes involved in many key organismal processes, including innate immunity, development, and stress responses. NF-κB proteins contain a highly conserved DNA-binding/dimerization domain called the Rel homology domain. Methods/Principal Findings We characterized two NF-κB alleles in the sea anemone Nematostella vectensis that differ at nineteen single-nucleotide polymorphisms (SNPs). Ten of these SNPs result in amino acid substitutions, including six within the Rel homology domain. Both alleles are found in natural populations of Nematostella. The relative abundance of the two NF-κB alleles differs between populations, and departures from Hardy-Weinberg equilibrium within populations indicate that the locus may be under selection. The proteins encoded by the two Nv-NF-κB alleles have different molecular properties, in part due to a Cys/Ser polymorphism at residue 67, which resides within the DNA recognition loop. In nearly all previously characterized NF-κB proteins, the analogous residue is fixed for Cys, and conversion of human RHD proteins from Cys to Ser at this site has been shown to increase DNA-binding ability and increase resistance to inhibition by thiol-reactive compounds. However, the naturally-occurring Nematostella variant with Cys at position 67 binds DNA with a higher affinity than the Ser variant. On the other hand, the Ser variant activates transcription in reporter gene assays more effectively, and it is more resistant to inhibition by a thiol-reactive compound. Reciprocal Cys<->Ser mutations at residue 67 of the native Nv-NF-κB proteins affect DNA binding as in human NF-κB proteins, e.g., a Cys->Ser mutation increases DNA binding of the native Cys variant. Conclusions/Significance These results are the first demonstration of a naturally occurring and functionally significant polymorphism in NF-κB in any species. The functional differences between these alleles and their uneven distribution in the wild suggest that different genotypes could be favored in different environments, perhaps environments that vary in their levels of peroxides or thiol-reactive compounds. PMID:19806194

  8. Ecological and morphological characteristics of Ephelota gemmipara (Ciliophora, Suctoria), epibiontic on Eudendrium racemosum (Cnidaria, Hydrozoa) from the Adriatic Sea.

    PubMed

    Tazioli, Silvia; Di Camillo, Cristina Gioia

    2013-11-01

    The relationship between the suctorian Ephelota gemmipara and the large hydroid Eudendrium racemosum from the North Adriatic Sea has been studied over its full annual cycle. Ephelota gemmipara settles on the perisarc of the hydroid, usually close to the hydranths in order to exploit the hydroid's food discharges. The life cycle of E. gemmipara is influenced by temperature variations and by its relationship with the host. The hydroid shows an active phase in the summer, and it gets through the adverse winter season forming resting stages. In April, when temperature increases, the hydroid starts its active phase and it is colonized by suctorians. From May to September the suctorians produce multiple buds (swarmers) that detach from the parental cells to settle on an Eudendrium colony. The abundance of the suctorian peaks in September, with more than 1.2 million ind. m(-2). Their proliferation coincides with the maximal abundance of their host and the highest water temperatures. On the contrary, sexual reproduction and the encystment occur when the temperature and the abundance of E. racemosum decrease. Lastly, we also report the presence of symbionts such as bacteria and the parasitic protozoans Tachyblaston ephelotensis and Enigmocoma acinetarum. PMID:23711377

  9. Multi-copy venom genes hidden in de novo transcriptome assemblies, a cautionary tale with the snakelocks sea anemone Anemonia sulcata (Pennant, 1977).

    PubMed

    Macrander, Jason; Broe, Michael; Daly, Marymegan

    2015-12-15

    Using a partial transcriptome of the snakelocks anemone (Anemonia sulcata) we identify toxin gene candidates that were incorrectly assembled into several Trinity components. Our approach recovers hidden diversity found within some toxin gene families that would otherwise go undetected when using Trinity, a widely used program for venom-focused transcriptome reconstructions. Unidentified hidden transcripts may significantly impact conclusions made regarding venom composition (or other multi-copy conserved genes) when using Trinity or other de novo assembly programs. PMID:26464059

  10. Taxonomic, Spatial and Temporal Patterns of Bleaching in Anemones Inhabited by Anemonefishes

    PubMed Central

    Hobbs, Jean-Paul A.; Frisch, Ashley J.; Ford, Benjamin M.; Thums, Michele; Saenz-Agudelo, Pablo; Furby, Kathryn A.; Berumen, Michael L.

    2013-01-01

    Background Rising sea temperatures are causing significant destruction to coral reef ecosystems due to coral mortality from thermally-induced bleaching (loss of symbiotic algae and/or their photosynthetic pigments). Although bleaching has been intensively studied in corals, little is known about the causes and consequences of bleaching in other tropical symbiotic organisms. Methodology/Principal Findings This study used underwater visual surveys to investigate bleaching in the 10 species of anemones that host anemonefishes. Bleaching was confirmed in seven anemone species (with anecdotal reports of bleaching in the other three species) at 10 of 19 survey locations spanning the Indo-Pacific and Red Sea, indicating that anemone bleaching is taxonomically and geographically widespread. In total, bleaching was observed in 490 of the 13,896 surveyed anemones (3.5%); however, this percentage was much higher (19–100%) during five major bleaching events that were associated with periods of elevated water temperatures and coral bleaching. There was considerable spatial variation in anemone bleaching during most of these events, suggesting that certain sites and deeper waters might act as refuges. Susceptibility to bleaching varied between species, and in some species, bleaching caused reductions in size and abundance. Conclusions/Significance Anemones are long-lived with low natural mortality, which makes them particularly vulnerable to predicted increases in severity and frequency of bleaching events. Population viability will be severely compromised if anemones and their symbionts cannot acclimate or adapt to rising sea temperatures. Anemone bleaching also has negative effects to other species, particularly those that have an obligate relationship with anemones. These effects include reductions in abundance and reproductive output of anemonefishes. Therefore, the future of these iconic and commercially valuable coral reef fishes is inextricably linked to the ability of host anemones to cope with rising sea temperatures associated with climate change. PMID:23951056

  11. Evolutionary crossroads in developmental biology: Cnidaria

    PubMed Central

    Technau, Ulrich; Steele, Robert E.

    2011-01-01

    There is growing interest in the use of cnidarians (corals, sea anemones, jellyfish and hydroids) to investigate the evolution of key aspects of animal development, such as the formation of the third germ layer (mesoderm), the nervous system and the generation of bilaterality. The recent sequencing of the Nematostella and Hydra genomes, and the establishment of methods for manipulating gene expression, have inspired new research efforts using cnidarians. Here, we present the main features of cnidarian models and their advantages for research, and summarize key recent findings using these models that have informed our understanding of the evolution of the developmental processes underlying metazoan body plan formation. PMID:21389047

  12. Understanding the molecular basis of toxin promiscuity: the analgesic sea anemone peptide APETx2 interacts with acid-sensing ion channel 3 and hERG channels via overlapping pharmacophores.

    PubMed

    Jensen, Jonas E; Cristofori-Armstrong, Ben; Anangi, Raveendra; Rosengren, K Johan; Lau, Carus H Y; Mobli, Mehdi; Brust, Andreas; Alewood, Paul F; King, Glenn F; Rash, Lachlan D

    2014-11-13

    The sea anemone peptide APETx2 is a potent and selective blocker of acid-sensing ion channel 3 (ASIC3). APETx2 is analgesic in a variety of rodent pain models, but the lack of knowledge of its pharmacophore and binding site on ASIC3 has impeded development of improved analogues. Here we present a detailed structure-activity relationship study of APETx2. Determination of a high-resolution structure of APETx2 combined with scanning mutagenesis revealed a cluster of aromatic and basic residues that mediate its interaction with ASIC3. We show that APETx2 also inhibits the off-target hERG channel by reducing the maximal current amplitude and shifting the voltage dependence of activation to more positive potentials. Electrophysiological screening of selected APETx2 mutants revealed partial overlap between the surfaces on APETx2 that mediate its interaction with ASIC3 and hERG. Characterization of the molecular basis of these interactions is an important first step toward the rational design of more selective APETx2 analogues. PMID:25337890

  13. Distribution of CpG Motifs in Upstream Gene Domains in a Reef Coral and Sea Anemone: Implications for Epigenetics in Cnidarians.

    PubMed

    Marsh, Adam G; Hoadley, Kenneth D; Warner, Mark E

    2016-01-01

    Coral reefs are under assault from stressors including global warming, ocean acidification, and urbanization. Knowing how these factors impact the future fate of reefs requires delineating stress responses across ecological, organismal and cellular scales. Recent advances in coral reef biology have integrated molecular processes with ecological fitness and have identified putative suites of temperature acclimation genes in a Scleractinian coral Acropora hyacinthus. We wondered what unique characteristics of these genes determined their coordinate expression in response to temperature acclimation, and whether or not other corals and cnidarians would likewise possess these features. Here, we focus on cytosine methylation as an epigenetic DNA modification that is responsive to environmental stressors. We identify common conserved patterns of cytosine-guanosine dinucleotide (CpG) motif frequencies in upstream promoter domains of different functional gene groups in two cnidarian genomes: a coral (Acropora digitifera) and an anemone (Nematostella vectensis). Our analyses show that CpG motif frequencies are prominent in the promoter domains of functional genes associated with environmental adaptation, particularly those identified in A. hyacinthus. Densities of CpG sites in upstream promoter domains near the transcriptional start site (TSS) are 1.38x higher than genomic background levels upstream of -2000 bp from the TSS. The increase in CpG usage suggests selection to allow for DNA methylation events to occur more frequently within 1 kb of the TSS. In addition, observed shifts in CpG densities among functional groups of genes suggests a potential role for epigenetic DNA methylation within promoter domains to impact functional gene expression responses in A. digitifera and N. vectensis. Identifying promoter epigenetic sequence motifs among genes within specific functional groups establishes an approach to describe integrated cellular responses to environmental stress in reef corals and potential roles of epigenetics on survival and fitness in the face of global climate change. PMID:26950882

  14. Distribution of CpG Motifs in Upstream Gene Domains in a Reef Coral and Sea Anemone: Implications for Epigenetics in Cnidarians

    PubMed Central

    Marsh, Adam G.; Hoadley, Kenneth D.; Warner, Mark E.

    2016-01-01

    Coral reefs are under assault from stressors including global warming, ocean acidification, and urbanization. Knowing how these factors impact the future fate of reefs requires delineating stress responses across ecological, organismal and cellular scales. Recent advances in coral reef biology have integrated molecular processes with ecological fitness and have identified putative suites of temperature acclimation genes in a Scleractinian coral Acropora hyacinthus. We wondered what unique characteristics of these genes determined their coordinate expression in response to temperature acclimation, and whether or not other corals and cnidarians would likewise possess these features. Here, we focus on cytosine methylation as an epigenetic DNA modification that is responsive to environmental stressors. We identify common conserved patterns of cytosine-guanosine dinucleotide (CpG) motif frequencies in upstream promoter domains of different functional gene groups in two cnidarian genomes: a coral (Acropora digitifera) and an anemone (Nematostella vectensis). Our analyses show that CpG motif frequencies are prominent in the promoter domains of functional genes associated with environmental adaptation, particularly those identified in A. hyacinthus. Densities of CpG sites in upstream promoter domains near the transcriptional start site (TSS) are 1.38x higher than genomic background levels upstream of -2000 bp from the TSS. The increase in CpG usage suggests selection to allow for DNA methylation events to occur more frequently within 1 kb of the TSS. In addition, observed shifts in CpG densities among functional groups of genes suggests a potential role for epigenetic DNA methylation within promoter domains to impact functional gene expression responses in A. digitifera and N. vectensis. Identifying promoter epigenetic sequence motifs among genes within specific functional groups establishes an approach to describe integrated cellular responses to environmental stress in reef corals and potential roles of epigenetics on survival and fitness in the face of global climate change. PMID:26950882

  15. Developmental and light-entrained expression of melatonin and its relationship to the circadian clock in the sea anemone Nematostella vectensis

    PubMed Central

    2014-01-01

    Background The primary hormone of the vertebrate pineal gland, melatonin, has been identified broadly throughout the eukaryotes. While the role for melatonin in cyclic behavior via interactions with the circadian clock has only been reported in vertebrates, comparative research has shown that the transcription-translation loops of the animal circadian clock likely date to the cnidarian-bilaterian ancestor, leaving open significant questions about the evolutionary origin of melatonin signaling in circadian behavior by interacting with the molecular clock. Results Expression of melatonin in adult anemones showed peak expression at the end of light period (zeitgeber time (ZT)?=?12) when cultured under diel conditions, coinciding with expression of genes and enzyme activity for members of the melatonin synthesis pathway (tryptophan hydroxylase and hydroxyindol-O-methyltransferase), which also showed rhythmic expression. During embryogenesis and juvenile stages, melatonin showed cyclic oscillations in concentration, peaking in midday. Spatial (in situ hybridization) and quantitative (real-time PCR) transcription of clock genes during development of N. vectensis showed these ‘clock’ genes are expressed early in the development, prior to rhythmic oscillations, suggesting functions independent of a function in the circadian clock. Finally, time-course studies revealed that animals transferred from diel conditions to constant darkness lose circadian expression for most of the clock genes within 4 days, which can be reset by melatonin supplementation. Conclusions Our results support an ancient role for melatonin in the circadian behavior of animals by showing cyclic expression of this hormone under diel conditions, light-dependent oscillations in genes in the melatonin synthesis pathway, and the function of melatonin in initiating expression of circadian clock genes in the cnidarian N. vectensis. The differences in expression melatonin and the circadian clock gene network in the adult stage when compared with developmental stages of N. vectensis suggests new research directions to characterize stage-specific mechanisms of circadian clock function in animals. PMID:25243057

  16. Looking for long-term changes in hydroid assemblages (Cnidaria, Hydrozoa) in Alboran Sea (South-Western Mediterranean): a proposal of a monitoring point for the global warming

    NASA Astrophysics Data System (ADS)

    González-Duarte, Manuel María; Megina, Cesar; Piraino, Stefano

    2014-12-01

    In the last 20-30 years, the temperature of the Mediterranean Sea has increased and global warming is allowing the establishment of tropical-affinity species into more temperate zones. Sessile communities are particularly useful as a baseline for ecological monitoring; however, a lack of historical data series exists for sessile marine organisms without commercial interest. Hydroids are ubiquitous components of the benthic sessile fauna on rocky shores and have been used as bio-indicators of environmental conditions. In this study on the benthic hydroid assemblages of the Chafarinas Islands (Alboran Sea, South-Western Mediterranean), we characterized the hydroid assemblages, identified the bathymetric gradients, and compared them with a previous study carried out in 1991. Hydroid assemblages showed a significant difference both between year and among depths. Furthermore, eight species not present in 1991 were found, including two possible new species and the tropical and subtropical species Sertularia marginata. Due to its strategic position at the entrance of the Mediterranean and the existence of previous data on hydroid assemblages, the Chafarinas Islands are proposed as a possible monitoring point for entrance of Atlantic tropical species into the Mediterranean Sea.

  17. Telopathes magna gen. nov., spec. nov. (Cnidaria: Anthozoa: Antipatharia: Schizopathidae) from deep waters off Atlantic Canada and the first molecular phylogeny of the deep-sea family Schizopathidae.

    PubMed

    Macisaac, K G; Best, M; Brugler, M R; Kenchington, E L R; Anstey, L J; Jordan, T

    2013-01-01

    A new genus and species of deep-sea antipatharian, Telopathes magna gen. nov., spec. nov., is described from the western North Atlantic off the coast of Canada. Five additional paratypes, consisting ofjuvenile to adult forms, are reported from the New England and Corner Rise Seamounts (NW Atlantic). Preliminary sequencing of a subsection of the nuclear ribosomal cistron confirmed the phylogenetic affinity of T. magna to the order Antipatharia, and in particular the family Schizopathidae. Subsequent sequencing of three mitochondrial DNA segments from nine of the 11 currently-recognized genera within the Schizopathidae revealed a well-supported phylogenetic relationship between T. magna and Stauropathes. This is the first study to use molecular techniques to elucidate the evolutionary relationships of the Schizopathidae, a family of black corals almost exclusively found in the deep sea (depths > 200 m). Telopathes is distinguished from other genera within the family Schizopathidae by its largely pinnulated stalk, sparse branching pattern to the second degree that is not restricted to a single plane, two anterolateral rows of long, simple primary pinnules, arranged alternately to sub-opposite, and colony with an adhesive base. This record of T. magna brings the total number of nominal species of Antipatharia reported to occur off eastern Canada to 12 and represents the third new genus added to the Schizopathidae since a critical review of the family by Dennis Opresko in 2002. PMID:26106725

  18. Interannual variability, growth, reproduction and feeding of Pelagia noctiluca (Cnidaria: Scyphozoa) in the Straits of Messina (Central Mediterranean Sea): Linkages with temperature and diet

    NASA Astrophysics Data System (ADS)

    Rosa, S.; Pansera, M.; Granata, A.; Guglielmo, L.

    2013-02-01

    To identify some of the possible environmental factors stimulating the increasingly frequent outbreaks of the scyphomedusa Pelagia noctiluca in the Straits of Messina, we investigated its abundance, growth, reproduction and feeding over a 4-year period, from 2007 to 2011, at two coastal sites. Using either field investigations and manipulative experiments we show that, among the various factors considered, shifts in water temperature (influencing medusae metabolism, growth and reproduction rates) and the size structure of the zooplankton community (their natural preys) can promote the proliferation of P. noctiluca. In particular, we show that increased temperature let jellyfishes to grow more rapidly and reach exceptional sizes. We also report a peculiar opportunistic behavior of P. noctiluca, which makes this species a potentially strong competitor in the pelagic trophic web of the Straits ecosystem. We therefore propose that more frequent P. noctiluca outbreaks stimulated by increasing sea surface temperature and shifts in their prey availability and composition would become, in the near future, a major cause of ecosystem shift.

  19. Evolutionary Diversification of Banded Tube-Dwelling Anemones (Cnidaria; Ceriantharia; Isarachnanthus) in the Atlantic Ocean

    PubMed Central

    Stampar, Sergio N.; Maronna, Maximiliano M.; Vermeij, Mark J. A.; Silveira, Fabio L. d.; Morandini, André C.

    2012-01-01

    The use of molecular data for species delimitation in Anthozoa is still a very delicate issue. This is probably due to the low genetic variation found among the molecular markers (primarily mitochondrial) commonly used for Anthozoa. Ceriantharia is an anthozoan group that has not been tested for genetic divergence at the species level. Recently, all three Atlantic species described for the genus Isarachnanthus of Atlantic Ocean, were deemed synonyms based on morphological simmilarities of only one species: Isarachnanthus maderensis. Here, we aimed to verify whether genetic relationships (using COI, 16S, ITS1 and ITS2 molecular markers) confirmed morphological affinities among members of Isarachnanthus from different regions across the Atlantic Ocean. Results from four DNA markers were completely congruent and revealed that two different species exist in the Atlantic Ocean. The low identification success and substantial overlap between intra and interspecific COI distances render the Anthozoa unsuitable for DNA barcoding, which is not true for Ceriantharia. In addition, genetic divergence within and between Ceriantharia species is more similar to that found in Medusozoa (Hydrozoa and Scyphozoa) than Anthozoa and Porifera that have divergence rates similar to typical metazoans. The two genetic species could also be separated based on micromorphological characteristics of their cnidomes. Using a specimen of Isarachnanthus bandanensis from Pacific Ocean as an outgroup, it was possible to estimate the minimum date of divergence between the clades. The cladogenesis event that formed the species of the Atlantic Ocean is estimated to have occured around 8.5 million years ago (Miocene) and several possible speciation scenarios are discussed. PMID:22815928

  20. Statolith formation in Cnidaria: effects of cadmium on Aurelia statoliths

    NASA Technical Reports Server (NTRS)

    Spangenberg, D. B.

    1986-01-01

    Statolith formation in Cnidaria was reviewed with an emphasis on Aurelia statoliths. The review provides information on the chemical composition, mechanisms of initiation of mineralization, and effects of environmental factors on Cnidarian statolith formation. Environmental factors discussed included modified sea water ingredients, X-irradiation, clinostat rotation, and petroleum oil ingredients. A detailed account of the effects of cadmium on mineralization and demineralization of Aurelia statoliths is given. Cadmium at dosages of 2 to 4 micromoles significantly reduces statolith numbers in developing ephyrae. At a dosage of 3 micromoles, cadmium accelerates statolith loss in unfed ephyrae studied at 4 and 8 days following ephyrae release from strobilae. Cadmium, therefore, is shown to reduce statolith numbers in developing ephyrae and to cause greater reduction of statolith numbers in unfed ephyrae after 4 and 8 days than occurred in controls. Supplementation of Cd(2+)-containing artificial sea water (ASW) with calcium (3X and 5X ASW calcium content) results in higher numbers of statoliths at day 4 as compared with cadmium-treated ephyrae. At 8 days only the 5X calcium supplemented ASW is effective in enhancing statolith numbers in Cd(2+)-treated ephyrae. These results suggest that cadmium competes in some manner with calcium at the mineralizing sites of Aurelia.

  1. Changes in zooxanthellae density, morphology, and mitotic index in hermatypic corals and anemones exposed to cyanide.

    PubMed

    Cervino, J M; Hayes, R L; Honovich, M; Goreau, T J; Jones, S; Rubec, P J

    2003-05-01

    Sodium cyanide (NaCN) is widely used for the capture of reef fish throughout Southeast Asia and causes extensive fish mortality, but the effect of NaCN on reef corals remains debated. To document the impact of cyanide exposure on corals, the species Acropora millepora, Goniopora sp., Favites abdita, Trachyphyllia geoffrio, Plerogyra sp., Heliofungia actinformis, Euphyllia divisa, and Scarophyton sp., and the sea anemone Aiptasia pallida were exposed to varying concentrations of cyanide for varying time periods. Corals were exposed to 50, 100, 300, and 600 mg/l of cyanide ion (CN(-)) for 1-2 min (in seawater, the CN(-) forms hydrocyanic acid). These concentrations are much lower than those reportedly used by fish collectors. Exposed corals and anemones immediately retracted their tentacles and mesenterial filaments, and discharged copious amounts of mucus containing zooxanthellae. Gel electrophoreses techniques found changes in protein expression in both zooxanthellae and host tissue. Corals and anemones exposed to cyanide showed an immediate increase in mitotic cell division of their zooxenthellae, and a decrease in zooxanthellae density. In contrast, zooxanthellae cell division and density remained constant in controls. Histopathological changes included gastrodermal disruption, mesogleal degradation, and increased mucus in coral tissues. Zooxanthellae showed pigment loss, swelling, and deformation. Mortality occurred at all exposure levels. Exposed specimens experienced an increase in the ratio of gram-negative to gram-positive bacteria on the coral surface. The results demonstrate that exposure cyanide causes mortality to corals and anemones, even when applied at lower levels than that used by fish collectors. Even brief exposure to cyanide caused slow-acting and long-term damage to corals and their zooxanthellae. PMID:12735955

  2. Deep-water Hydrozoa (Cnidaria: Medusozoa) in the Sea of Japan, collected during the 51st Cruise of R/V Akademik M.A. Lavrentyev, with description Opercularella angelikae, sp. nov.

    NASA Astrophysics Data System (ADS)

    Stepanjants, Sofia D.

    2013-02-01

    A report is given about Hydrozoa collected at depths between 455 and 3666 m in the Sea of Japan during the Russian-German expedition on R/V Akademik M.A. Lavrentyev. Ten species were found, with four of them being typical bathyal-abyssal and abyssal zones. A new species, Opercularella angelikae, is described, and it was the dominant hydroid in samples from 970 to 3660 m. Four eurybathic species characteristics of the Sea of Japan were sampled between 455 and 582 m. Abyssal (pseudoabyssal after Andriashev, 1979) hydroid fauna in the Sea of Japan is reported. The hypothesis that an exclusively deep-water fauna is lacking in abyssal regions of the Sea of Japan is disputed. The author's personal opinion considered concerning the borders of 1000 m between shallow and deep hydrozoan species in the Sea of Japan.

  3. Macrofouling of deep-sea instrumentation after three years at 3690 m depth in the Charlie Gibbs fracture zone, mid-Atlantic ridge, with emphasis on hydroids (Cnidaria: Hydrozoa)

    NASA Astrophysics Data System (ADS)

    Blanco, R.; Shields, M. A.; Jamieson, A. J.

    2013-12-01

    Macrofouling is a common problem when deploying underwater instrumentation for long periods of time. It is a problem which can effect scientific experiments and monitoring missions though the creation of artificial reefs (thus increasing local biological activity) and reduce the quality of scientific data. Macrofouling is an issue typically considered to be restricted to the photic zones and is absent or negligible in the deep sea. To the contrary, the recovery of an accidentally lost deep-sea lander after 3 years submergence at 3960 m on the Mid-Atlantic Ridge (North Atlantic) revealed dense colonisation of macrofouling organisms. These organisms were found attached to all surfaces of the lander regardless of orientation and materials. The occurrence of such deep-sea macrofouling should be carefully investigated given the recent developments in long-term deep-sea observatory networks.

  4. AdE-1, a new inotropic Na(+) channel toxin from Aiptasia diaphana, is similar to, yet distinct from, known anemone Na(+) channel toxins.

    PubMed

    Nesher, Nir; Shapira, Eli; Sher, Daniel; Moran, Yehu; Tsveyer, Liora; Turchetti-Maia, Ana Luiza; Horowitz, Michal; Hochner, Binyamin; Zlotkin, Eliahu

    2013-04-01

    Heart failure is one of the most prevalent causes of death in the western world. Sea anemone contains a myriad of short peptide neurotoxins affecting many pharmacological targets, several of which possess cardiotonic activity. In the present study we describe the isolation and characterization of AdE-1 (ion channel modifier), a novel cardiotonic peptide from the sea anemone Aiptasia diaphana, which differs from other cnidarian toxins. Although AdE-1 has the same cysteine residue arrangement as sea anemone type 1 and 2 Na(+) channel toxins, its sequence contains many substitutions in conserved and essential sites and its overall homology to other toxins identified to date is low (<36%). Physiologically, AdE-1 increases the amplitude of cardiomyocyte contraction and slows the late phase of the twitch relaxation velocity with no induction of spontaneous twitching. It increases action potential duration of cardiomyocytes with no effect on its threshold and on the cell's resting potential. Similar to other sea anemone Na(+) channel toxins such as Av2 (Anemonia viridis toxin II), AdE-1 markedly inhibits Na(+) current inactivation with no significant effect on current activation, suggesting a similar mechanism of action. However, its effects on twitch relaxation velocity, action potential amplitude and on the time to peak suggest that this novel toxin affects cardiomyocyte function via a more complex mechanism. Additionally, Av2's characteristic delayed and early after-depolarizations were not observed. Despite its structural differences, AdE-1 physiologic effectiveness is comparable with Av2 with a similar ED(50) value to blowfly larvae. This finding raises questions regarding the extent of the universality of structure-function in sea anemone Na(+) channel toxins. PMID:23356888

  5. The Mucus of Actinia equina (Anthozoa, Cnidaria): An Unexplored Resource for Potential Applicative Purposes.

    PubMed

    Stabili, Loredana; Schirosi, Roberto; Parisi, Maria Giovanna; Piraino, Stefano; Cammarata, Matteo

    2015-08-01

    The mucus produced by many marine organisms is a complex mixture of proteins and polysaccharides forming a weak watery gel. It is essential for vital processes including locomotion, navigation, structural support, heterotrophic feeding and defence against a multitude of environmental stresses, predators, parasites, and pathogens. In the present study we focused on mucus produced by a benthic cnidarian, the sea anemone Actinia equina (Linnaeus, 1758) for preventing burial by excess sedimentation and for protection. We investigated some of the physico-chemical properties of this matrix such as viscosity, osmolarity, electrical conductivity, protein, carbohydrate, and total lipid contents. Some biological activities such as hemolytic, cytotoxic, and antibacterial lysozyme-like activities were also studied. The A. equina mucus is mainly composed by water (96.2% ± 0.3%), whereas its dry weight is made of 24.2% ± 1.3% proteins and 7.8% ± 0.2% carbohydrates, with the smallest and largest components referable to lipids (0.9%) and inorganic matter (67.1%). The A. equina mucus matrix exhibited hemolytic activity on rabbit erythrocytes, cytotoxic activity against the tumor cell line K562 (human erythromyeloblastoid leukemia) and antibacterial lysozyme-like activity. The findings from this study improve the available information on the mucus composition in invertebrates and have implications for future investigations related to exploitation of A. equina and other sea anemones' mucus as a source of bioactive compounds of high pharmaceutical and biotechnological interest. PMID:26295400

  6. Phylogenomic Analyses Support Traditional Relationships within Cnidaria

    PubMed Central

    Zapata, Felipe; Goetz, Freya E.; Smith, Stephen A.; Howison, Mark; Siebert, Stefan; Church, Samuel H.; Sanders, Steven M.; Ames, Cheryl Lewis; McFadden, Catherine S.; France, Scott C.; Daly, Marymegan; Collins, Allen G.; Haddock, Steven H. D.; Dunn, Casey W.; Cartwright, Paulyn

    2015-01-01

    Cnidaria, the sister group to Bilateria, is a highly diverse group of animals in terms of morphology, lifecycles, ecology, and development. How this diversity originated and evolved is not well understood because phylogenetic relationships among major cnidarian lineages are unclear, and recent studies present contrasting phylogenetic hypotheses. Here, we use transcriptome data from 15 newly-sequenced species in combination with 26 publicly available genomes and transcriptomes to assess phylogenetic relationships among major cnidarian lineages. Phylogenetic analyses using different partition schemes and models of molecular evolution, as well as topology tests for alternative phylogenetic relationships, support the monophyly of Medusozoa, Anthozoa, Octocorallia, Hydrozoa, and a clade consisting of Staurozoa, Cubozoa, and Scyphozoa. Support for the monophyly of Hexacorallia is weak due to the equivocal position of Ceriantharia. Taken together, these results further resolve deep cnidarian relationships, largely support traditional phylogenetic views on relationships, and provide a historical framework for studying the evolutionary processes involved in one of the most ancient animal radiations. PMID:26465609

  7. Molecular phylogenetics of the siphonophora (Cnidaria), with implications for the evolution of functional specialization.

    PubMed

    Dunn, Casey W; Pugh, Philip R; Haddock, Steven H D

    2005-12-01

    Siphonophores are a group of pelagic colonial hydrozoans (Cnidaria) that have long been of general interest because of the division of labor between the polyps and medusae that make up these "superorganisms." These polyps and medusae are each homologous to free living animals but are generated by an incomplete asexual budding process that leaves them physiologically integrated. They are functionally specialized for different tasks and are precisely organized within each colony. The number of functional types of polyps and medusae varies across taxa, and different authors have used this character to construct phylogenies polarized in opposite directions, depending on whether they thought siphonophore evolution proceeded by a reduction or an increase in functional specialization. We have collected taxa across all major groups of siphonophores, many of which are found exclusively in the deep sea, using remotely operated underwater vehicles (ROVs) and by SCUBA diving from ships in the open ocean. We have used 52 siphonophores and four outgroup taxa to estimate the siphonophore phylogeny with molecular data from the nuclear small subunit ribosomal RNA gene (18S) and the mitochondrial large subunit ribosomal RNA gene (16S). Parsimony reconstructions indicate that functionally specialized polyps and medusae have been gained and lost across the phylogeny. Maximum likelihood and Bayesian analyses of morphological data suggest that the transition rate for decreased functional specialization is greater than the transition rate for increased functional specialization for three out of the four investigated categories of polyps and medusae. The present analysis also bears on several long-standing questions about siphonophore systematics. It indicates that the cystonects are sister to all other siphonophores, a group that we call the Codonophora. We also find that the Calycophorae are nested within the Physonectae, and that the Brachystelia, a historically recognized grouping of short-stemmed taxa, are polyphyletic. [Cnidaria; colonial animals; deep sea; division of labor; functional specialization; Hydrozoa; phylogenetics; Siphonophores.]. PMID:16338764

  8. Genomic insights into the evolutionary origin of Myxozoa within Cnidaria

    PubMed Central

    Chang, E. Sally; Neuhof, Moran; Rubinstein, Nimrod D.; Diamant, Arik; Philippe, Hervé; Huchon, Dorothée; Cartwright, Paulyn

    2015-01-01

    The Myxozoa comprise over 2,000 species of microscopic obligate parasites that use both invertebrate and vertebrate hosts as part of their life cycle. Although the evolutionary origin of myxozoans has been elusive, a close relationship with cnidarians, a group that includes corals, sea anemones, jellyfish, and hydroids, is supported by some phylogenetic studies and the observation that the distinctive myxozoan structure, the polar capsule, is remarkably similar to the stinging structures (nematocysts) in cnidarians. To gain insight into the extreme evolutionary transition from a free-living cnidarian to a microscopic endoparasite, we analyzed genomic and transcriptomic assemblies from two distantly related myxozoan species, Kudoa iwatai and Myxobolus cerebralis, and compared these to the transcriptome and genome of the less reduced cnidarian parasite, Polypodium hydriforme. A phylogenomic analysis, using for the first time to our knowledge, a taxonomic sampling that represents the breadth of myxozoan diversity, including four newly generated myxozoan assemblies, confirms that myxozoans are cnidarians and are a sister taxon to P. hydriforme. Estimations of genome size reveal that myxozoans have one of the smallest reported animal genomes. Gene enrichment analyses show depletion of expressed genes in categories related to development, cell differentiation, and cell–cell communication. In addition, a search for candidate genes indicates that myxozoans lack key elements of signaling pathways and transcriptional factors important for multicellular development. Our results suggest that the degeneration of the myxozoan body plan from a free-living cnidarian to a microscopic parasitic cnidarian was accompanied by extreme reduction in genome size and gene content. PMID:26627241

  9. Genomic insights into the evolutionary origin of Myxozoa within Cnidaria.

    PubMed

    Chang, E Sally; Neuhof, Moran; Rubinstein, Nimrod D; Diamant, Arik; Philippe, Hervé; Huchon, Dorothée; Cartwright, Paulyn

    2015-12-01

    The Myxozoa comprise over 2,000 species of microscopic obligate parasites that use both invertebrate and vertebrate hosts as part of their life cycle. Although the evolutionary origin of myxozoans has been elusive, a close relationship with cnidarians, a group that includes corals, sea anemones, jellyfish, and hydroids, is supported by some phylogenetic studies and the observation that the distinctive myxozoan structure, the polar capsule, is remarkably similar to the stinging structures (nematocysts) in cnidarians. To gain insight into the extreme evolutionary transition from a free-living cnidarian to a microscopic endoparasite, we analyzed genomic and transcriptomic assemblies from two distantly related myxozoan species, Kudoa iwatai and Myxobolus cerebralis, and compared these to the transcriptome and genome of the less reduced cnidarian parasite, Polypodium hydriforme. A phylogenomic analysis, using for the first time to our knowledge, a taxonomic sampling that represents the breadth of myxozoan diversity, including four newly generated myxozoan assemblies, confirms that myxozoans are cnidarians and are a sister taxon to P. hydriforme. Estimations of genome size reveal that myxozoans have one of the smallest reported animal genomes. Gene enrichment analyses show depletion of expressed genes in categories related to development, cell differentiation, and cell-cell communication. In addition, a search for candidate genes indicates that myxozoans lack key elements of signaling pathways and transcriptional factors important for multicellular development. Our results suggest that the degeneration of the myxozoan body plan from a free-living cnidarian to a microscopic parasitic cnidarian was accompanied by extreme reduction in genome size and gene content. PMID:26627241

  10. Searching for a Toxic Key to Unlock the Mystery of Anemonefish and Anemone Symbiosis

    PubMed Central

    Nedosyko, Anita M.; Young, Jeanne E.; Edwards, John W.; Burke da Silva, Karen

    2014-01-01

    Twenty-six species of anemonefish of the genera Amphiprion and monospecific Premnas, use only 10 species of anemones as hosts in the wild (Families: Actiniidae, Stichodactylidae and Thalassianthidae). Of these 10 anemone species some are used by multiple species of anemonefish while others have only a single anemonefish symbiont. Past studies have explored the different patterns of usage between anemonefish species and anemone species; however the evolution of this relationship remains unknown and has been little studied over the past decade. Here we reopen the case, comparing the toxicity of crude venoms obtained from anemones that host anemonefish as a way to investigate why some anemone species are used as a host more than others. Specifically, for each anemone species we investigated acute toxicity using Artemia francisca (LC50), haemolytic toxicity using ovine erythrocytes (EC50) and neurotoxicity using shore crabs (Ozius truncatus). We found that haemolytic and neurotoxic activity varied among host anemone species. Generally anemone species that displayed greater haemolytic activity also displayed high neurotoxic activity and tend to be more toxic on average as indicated by acute lethality analysis. An overall venom toxicity ranking for each anemone species was compared with the number of anemonefish species that are known to associate with each anemone species in the wild. Interestingly, anemones with intermediate toxicity had the highest number of anemonefish associates, whereas anemones with either very low or very high toxicity had the fewest anemonefish associates. These data demonstrate that variation in toxicity among host anemone species may be important in the establishment and maintenance of anemonefish anemone symbiosis. PMID:24878777

  11. STATISTICAL STUDY OF CHROMOSPHERIC ANEMONE JETS OBSERVED WITH HINODE/SOT

    SciTech Connect

    Nishizuka, N.; Nakamura, T.; Kawate, T.; Singh, K. A. P.; Shibata, K.

    2011-04-10

    The Solar Optical Telescope on board Hinode has revealed numerous tiny jets in all regions of the chromosphere outside of sunspots. A typical chromospheric anemone jet has a cusp-shaped structure and bright footpoint, similar to the shape of an X-ray anemone jet observed previously with the Soft X-ray Telescope on board Yohkoh. The similarity in the shapes of chromospheric and X-ray anemone jets suggests that chromospheric anemone jets are produced as a result of the magnetic reconnection between a small bipole (perhaps a tiny emerging flux) and a pre-existing uniform magnetic field in the lower chromosphere. We examine various chromospheric anemone jets in the solar active region near the solar limb and study the typical features (e.g., length, width, lifetime, and velocity) of the chromospheric anemone jets. Statistical studies show that chromospheric anemone jets have: (1) a typical length {approx}1.0-4.0 Mm, (2) a width {approx}100-400 km, (3) a lifetime {approx}100-500 s, and (4) a velocity {approx}5-20 km s{sup -1}. The velocity of the chromospheric anemone jets is comparable to the local Alfven speed in the lower solar chromosphere ({approx}10 km s{sup -1}). The histograms of chromospheric anemone jets near the limb and near the disk center show similar averages and shapes of distributions, suggesting that the characteristic behavior of chromospheric anemone jets is independent of whether they are observed on the disk or at the limb. The observed relationship between the velocity and length of chromospheric anemone jets shows that the jets do not follow ballistic motion but are more likely accelerated by some other mechanism. This is consistent with numerical simulations of chromospheric anemone jets.

  12. Elucidating the evolutionary relationships of the Aiptasiidae, a widespread cnidarian-dinoflagellate model system (Cnidaria: Anthozoa: Actiniaria: Metridioidea).

    PubMed

    Grajales, Alejandro; Rodríguez, Estefanía

    2016-01-01

    Sea anemones of the family Aiptasiidae sensu Grajales and Rodríguez (2014) are conspicuous members of shallow-water environments, including several species widely used as model systems for the study of cnidarian-dinoflagellate symbiosis and coral bleaching. Although previously published phylogenetic studies of sea anemones recovered Aiptasiidae as polyphyletic, they only included a sparse sample in terms of its taxonomic diversity and membership of the family had not been yet revised. This study explores the phylogenetic relationships of this family using five molecular markers and including newly collected material from the geographical distribution of most of the currently described genera and species. We find a monophyletic family Aiptasiidae. All the currently proposed genera were recovered as monophyletic units, a finding also supported by diagnostic morphological characters. Our results confirm Bellactis and Laviactis as members of Aiptasiidae, also in agreement with previous morphological studies. The monophyly of the group is congruent with the morphological homogeneity of the members of this family. The obtained results also allow discussing the evolution of morphological characters within the family. Furthermore, we find evidence for and describe a new cryptic species, Exaiptasia brasiliensis sp. nov., based on molecular data, geographical distribution, and the identity of its endosymbiotic dinoflagellate. PMID:26375331

  13. Chromospheric anemone jets as evidence of ubiquitous reconnection.

    PubMed

    Shibata, Kazunari; Nakamura, Tahei; Matsumoto, Takuma; Otsuji, Kenichi; Okamoto, Takenori J; Nishizuka, Naoto; Kawate, Tomoko; Watanabe, Hiroko; Nagata, Shin'ichi; Ueno, Satoru; Kitai, Reizaburo; Nozawa, Satoshi; Tsuneta, Saku; Suematsu, Yoshinori; Ichimoto, Kiyoshi; Shimizu, Toshifumi; Katsukawa, Yukio; Tarbell, Theodore D; Berger, Thomas E; Lites, Bruce W; Shine, Richard A; Title, Alan M

    2007-12-01

    The heating of the solar chromosphere and corona is a long-standing puzzle in solar physics. Hinode observations show the ubiquitous presence of chromospheric anemone jets outside sunspots in active regions. They are typically 3 to 7 arc seconds = 2000 to 5000 kilometers long and 0.2 to 0.4 arc second = 150 to 300 kilometers wide, and their velocity is 10 to 20 kilometers per second. These small jets have an inverted Y-shape, similar to the shape of x-ray anemone jets in the corona. These features imply that magnetic reconnection similar to that in the corona is occurring at a much smaller spatial scale throughout the chromosphere and suggest that the heating of the solar chromosphere and corona may be related to small-scale ubiquitous reconnection. PMID:18063790

  14. Lipids of gelatinous Antarctic zooplankton: Cnidaria and Ctenophora.

    PubMed

    Nelson, M M; Phleger, C F; Mooney, B D; Nichols, P D

    2000-05-01

    Cnidaria (Calycopsis borchgrevinki, Diphyes antarctica, Stygiomedusa gigantea, Atolla wyvillei, Dimophyes arctica) and Ctenophora (Beroe cucumis, B. forskalii, Pleurobrachia pileus, Bolinopsis infundibulum) were collected near Elephant Island, South Shetland Islands, during January and February 1997 and 1998. Total lipid was low in all zooplankton (0.1-5 mg g wet mass) and included primarily polar lipids (59-96% of total lipid). Triacylglycerols were 0-26% of total lipids, and wax esters were 0-11% in all species. Cholesterol was the major sterol in all Cnidaria (50-63% of total sterols) whereas in most ctenophores it was lower at 26-45%. These cholesterol levels are consistent with a combined carnivorous and phytoplanktivorous diet in the ctenophores, with the carnivorous diet more dominant in the Cnidaria. Other sterols included primarily trans-dehydrocholesterol, desmosterol, 24-methylcholest-5,22E-dien-3beta-ol, 24-nordehydrocholesterol, and 24-methylenecholesterol. Total stanols were 0-6% in all zooplankton. Eicosapentaenoic acid and docosahexaenoic acid were the major polyunsaturated fatty acids (PUFA) in all samples (7-25% of total fatty acids) except for A. wyvillei in which docosapentaenoic acid was 10% of total fatty acids. The PUFA 18:5n-3 was not detected in 1997 samples, but constituted 0.2-0.8% in most 1998 samples. Monounsaturated fatty acids included primarily 18:1n-9c, 16:1n-7c, and 18:1n-7c. The principal saturated fatty acids in all samples were 16:0, 18:0, and 14:0. These data are the first for many of these zooplankton species and the first sterol data for most species. The use of the signature lipid approach has enabled examination of aspects of trophodynamics not obtainable by conventional techniques. PMID:10907790

  15. Overview of the genus Briareum (Cnidaria, Octocorallia, Briareidae) in the Indo-Pacific, with the description of a new species

    PubMed Central

    Samimi-Namin, Kaveh; van Ofwegen, Leen P.

    2016-01-01

    Abstract The status of Indo-Pacific Briareum species (Cnidaria, Octocorallia, Briareidae) is reviewed by presenting their sclerite features and habitus descriptions. Following the re-examination of type material, museum specimens and newly collected specimens, a species identification key is provided. The species distributions are discussed and updated distribution ranges are depicted. Moreover, a new taxon, Briareum cylindrum sp. n. is described and depicted, whereas Briareum excavatum (Nutting, 1911) is synonymised with Briareum stechei (Kükenthal, 1908). Briareum hamrum (Gohar, 1948) is recorded from the Persian Gulf and Oman Sea for the first time. Consequently, in total four Briareum species are recognized in the Indo-Pacific; Briareum hamrum from the western Indian Ocean, and Briareum cylindrum sp. n., Briareum stechei, and Briareum violaceum from the central and eastern Indo-Pacific region. PMID:26877697

  16. Overview of the genus Briareum (Cnidaria, Octocorallia, Briareidae) in the Indo-Pacific, with the description of a new species.

    PubMed

    Samimi-Namin, Kaveh; van Ofwegen, Leen P

    2016-01-01

    The status of Indo-Pacific Briareum species (Cnidaria, Octocorallia, Briareidae) is reviewed by presenting their sclerite features and habitus descriptions. Following the re-examination of type material, museum specimens and newly collected specimens, a species identification key is provided. The species distributions are discussed and updated distribution ranges are depicted. Moreover, a new taxon, Briareum cylindrum sp. n. is described and depicted, whereas Briareum excavatum (Nutting, 1911) is synonymised with Briareum stechei (Kükenthal, 1908). Briareum hamrum (Gohar, 1948) is recorded from the Persian Gulf and Oman Sea for the first time. Consequently, in total four Briareum species are recognized in the Indo-Pacific; Briareum hamrum from the western Indian Ocean, and Briareum cylindrum sp. n., Briareum stechei, and Briareum violaceum from the central and eastern Indo-Pacific region. PMID:26877697

  17. Visual ecology and functional morphology of cubozoa (cnidaria).

    PubMed

    Coates, Melissa M

    2003-08-01

    Jellyfish belong to one of the oldest extant animal phyla, the Cnidaria. The first Cnidaria appear in the fossil record 600 million years ago, preceeding the Cambrian explosion. They are an extremely successful group present in all marine environments and some freshwater environments. In contrast to many animal phyla in which vision is a primary sense Cnidarians do not, generally, employ image forming eyes. One small class stands alone: the Cubozoa. Cubomedusae are commonly known as box jellyfish. They possess image forming eyes (Coates et al., 2001) which certainly evolved independently from other metazoans. Cubomedusae therefore offer a unique perspective on the evolution of image forming eyes. This literature review collects, into one place, what is known about: the multiple eye types of box jellyfish, cubomedusan life history and ecology, and the sensory and neural systems of box jellyfish. Here I discuss how these features set cubomedusae apart from scyphomedusae and hydromedusae. Knowledge in these areas is sparse; the work done to date inspires increased efforts. PMID:21680462

  18. The Mucus of Actinia equina (Anthozoa, Cnidaria): An Unexplored Resource for Potential Applicative Purposes

    PubMed Central

    Stabili, Loredana; Schirosi, Roberto; Parisi, Maria Giovanna; Piraino, Stefano; Cammarata, Matteo

    2015-01-01

    The mucus produced by many marine organisms is a complex mixture of proteins and polysaccharides forming a weak watery gel. It is essential for vital processes including locomotion, navigation, structural support, heterotrophic feeding and defence against a multitude of environmental stresses, predators, parasites, and pathogens. In the present study we focused on mucus produced by a benthic cnidarian, the sea anemone Actinia equina (Linnaeus, 1758) for preventing burial by excess sedimentation and for protection. We investigated some of the physico-chemical properties of this matrix such as viscosity, osmolarity, electrical conductivity, protein, carbohydrate, and total lipid contents. Some biological activities such as hemolytic, cytotoxic, and antibacterial lysozyme-like activities were also studied. The A. equina mucus is mainly composed by water (96.2% ± 0.3%), whereas its dry weight is made of 24.2% ± 1.3% proteins and 7.8% ± 0.2% carbohydrates, with the smallest and largest components referable to lipids (0.9%) and inorganic matter (67.1%). The A. equina mucus matrix exhibited hemolytic activity on rabbit erythrocytes, cytotoxic activity against the tumor cell line K562 (human erythromyeloblastoid leukemia) and antibacterial lysozyme-like activity. The findings from this study improve the available information on the mucus composition in invertebrates and have implications for future investigations related to exploitation of A. equina and other sea anemones’ mucus as a source of bioactive compounds of high pharmaceutical and biotechnological interest. PMID:26295400

  19. Transmission genetics of allorecognition in Hydractinia symbiolongicarpus (Cnidaria: Hydrozoa)

    SciTech Connect

    Mokady, O.; Buss, L.W.

    1996-06-01

    Allorecognition is ubiquitous, or nearly so, amongst colonial invertebrates. Despite the prominent role that such phenomena have played both in evolutionary theory and in speculations on the origin of the vertebrate immune system, unambiguous data on the transmission genetics of fusibility (i.e., the ability of two individuals to fuse upon tissue contact) is lacking for any metazoan outside of the phylum Chordata. We have developed lines of the hydroid Hydractinia symbiolongicarpus (Phylum Cnidaria) inbred for fusibility and here report results of breeding experiments establishing that fusibility segregates as expected for a single locus with codominantly expressed alleles, with one shared allele producing a fusible phenotype. Surveys of fusibility in field populations and additional breeding experiments indicate the presence of an extensive allele series. 21 refs., 1 fig., 3 tabs.

  20. Analysis of complete mitochondrial DNA sequences of three members of the Montastraea annularis coral species complex (Cnidaria, Anthozoa, Scleractinia)

    NASA Astrophysics Data System (ADS)

    Fukami, Hironobu; Knowlton, Nancy

    2005-11-01

    Complete mitochondrial nucleotide sequences of two individuals each of Montastraea annularis, Montastraea faveolata, and Montastraea franksi were determined. Gene composition and order differed substantially from the sea anemone Metridium senile, but were identical to that of the phylogenetically distant coral genus Acropora. However, characteristics of the non-coding regions differed between the two scleractinian genera. Among members of the M. annularis complex, only 25 of 16,134 base pair positions were variable. Sixteen of these occurred in one colony of M. franksi, which (together with additional data) indicates the existence of multiple divergent mitochondrial lineages in this species. Overall, rates of evolution for these mitochondrial genomes were extremely slow (0.03 0.04% per million years based on the fossil record of the M. annularis complex). At higher taxonomic levels, patterns of genetic divergence and synonymous/nonsynonymous substitutions suggest non-neutral and unequal rates of evolution between the two lineages to which Montastraea and Acropora belong.

  1. Circadian Clocks in the Cnidaria: Environmental Entrainment, Molecular Regulation, and Organismal Outputs

    PubMed Central

    Reitzel, Adam M.; Tarrant, Ann M.; Levy, Oren

    2013-01-01

    The circadian clock is a molecular network that translates predictable environmental signals, such as light levels, into organismal responses, including behavior and physiology. Regular oscillations of the molecular components of the clock enable individuals to anticipate regularly fluctuating environmental conditions. Cnidarians play important roles in benthic and pelagic marine environments and also occupy a key evolutionary position as the likely sister group to the bilaterians. Together, these attributes make members of this phylum attractive as models for testing hypotheses on roles for circadian clocks in regulating behavior, physiology, and reproduction as well as those regarding the deep evolutionary conservation of circadian regulatory pathways in animal evolution. Here, we review and synthesize the field of cnidarian circadian biology by discussing the diverse effects of daily light cycles on cnidarians, summarizing the molecular evidence for the conservation of a bilaterian-like circadian clock in anthozoan cnidarians, and presenting new empirical data supporting the presence of a conserved feed-forward loop in the starlet sea anemone, Nematostella vectensis. Furthermore, we discuss critical gaps in our current knowledge about the cnidarian clock, including the functions directly regulated by the clock and the precise molecular interactions that drive the oscillating gene-expression patterns. We conclude that the field of cnidarian circadian biology is moving rapidly toward linking molecular mechanisms with physiology and behavior. PMID:23620252

  2. New phylogenomic and comparative analyses provide corroborating evidence that Myxozoa is Cnidaria.

    PubMed

    Feng, Jin-Mei; Xiong, Jie; Zhang, Jin-Yong; Yang, Ya-Lin; Yao, Bin; Zhou, Zhi-Gang; Miao, Wei

    2014-12-01

    Myxozoa, a diverse group of morphologically simplified endoparasites, are well known fish parasites causing substantial economic losses in aquaculture. Despite active research, the phylogenetic position of Myxozoa remains ambiguous. After obtaining the genome and transcriptome data of the myxozoan Thelohanellus kitauei, we examined the phylogenetic position of Myxozoa from three different perspectives. First, phylogenomic analyses with the newly sequenced genomic data strongly supported the monophyly of Myxozoa and that Myxozoa is sister to Medusozoa within Cnidaria. Second, we detected two homologs to cnidarian-specific minicollagens in the T. kitauei genome with molecular characteristics similar to cnidarian-specific minicollagens, suggesting that the minicollagen homologs in T. kitauei may have functions similar to those in Cnidaria and that Myxozoa is Cnidaria. Additionally, phylogenetic analyses revealed that the minicollagens in myxozoans and medusozoans have a common ancestor. Third, we detected 11 of the 19 proto-mesodermalgenes in the T. kitauei genome, which were also present in the cnidarian Hydra magnipapillata, indicating Myxozoa is within Cnidaria. Thus, our results robustly support Myxozoa as a derived cnidarian taxon with an affinity to Medusozoa, helping to understand the diversity of the morphology, development and life cycle of Cnidaria and its evolution. PMID:25192780

  3. CHARACTERIZATION OF MICROSATELLITE LOCI IN THE WIDELY INTRODUCED ESTUARINE ANEMONE NEMATOSTELLA VECTENSIS

    EPA Science Inventory

    We characterized ten polymorphic microsatellite loci from Nematostella vectensis, a burrowing anemone recently introduced to estuaries along the Pacific coast of North America and the southeast coast of England. Preliminary results indicate high variability and significant depar...

  4. The evolution of colony-level development in the Siphonophora (Cnidaria:Hydrozoa).

    PubMed

    Dunn, Casey W; Wagner, Günter P

    2006-12-01

    Evolutionary developmental biology has focused almost exclusively on multicellular organisms, but there are other relevant levels of biological organization that have remained largely neglected. Animal colonies are made up of multiple physiologically integrated and genetically identical units called zooids that are each homologous to solitary, free-living animals. Siphonophores, a group of pelagic hydrozoans (Cnidaria), have the most complex colony-level organization of all animals. Here the colony-level development of five siphonophore species, strategically sampled across the siphonophore phylogeny, is described from specimens collected using deep-sea submersibles and by self-contained underwater breathing apparatus diving. These species include three cystonects, Bathyphysa sibogae, Rhizophysa filiformis, and Rhizophysa eysenhardti, and two "physonects", Agalma elegans and Nanomia bijuga. These data, together with previous findings, are analyzed in a phylogenetic framework to reconstruct key features of the history of colony-level organization and development in the Siphonophora. It is shown that gonodendra and gastrozooids of the examined cystonects arise as independent buds directly on the stem, whereas probud subdivision (the origin of feeding, reproductive, and other zooids from a single bud) is a synapomorphy of the Codonophora. The origin of probud subdivision is associated with the origin of cormidia as integrated units of colony organization, and may have allowed for greater morphological and ecological diversification in the Codonophora relative to the Cystonectae. It is also found that symmetry is labile in siphonophores, with multiple gains and/or losses of directional asymmetry in the group. This descriptive work will enable future mechanistic and molecular studies of colony-level development in the siphonophores. PMID:16983540

  5. A symbiogenetic theory for the origins of cnidocysts in Cnidaria.

    PubMed

    Shostak, S

    1993-01-01

    Did cnidarian cnidocysts originate from cnidocyst-bearing protoctistans living as symbiotic partners with an epithelial placula? If an increase in the fitness of symbiotic partners was "locked in" by an evolutionary stable strategy, co-evolution and compartmentalization could have led phyletically separate, eukaryotic symbionts to fuse and undergo nuclear merger. Traits originating in the symbiotic partners would have been brought to the "synthetic" organism and reworked through evolution into the development of an integrated organism. Support for the theory of symbiogenetic origins of Cnidaria rests on traces of symbiosis detected in the relationship of cnidarian epithelium to interstitial cells (I-cells), the precursors of cnidocyst-producing cnidoblasts: (1) epithelium and I-cell are autonomous and differ in morphology, cellular dynamics, the relationship of differentiation to proliferation and the variety of cell types formed; (2) hydras and planulas can be "cured" of I-cells and their derivatives, thereby creating "epithelial" animals which lack responsiveness but retain vegetative properties. (3) The reintroduction of I-cells into "epithelial" animals which lack responsiveness but retain vegetative properties. (3) The reintroduction of I-cells into "epithelial" animals restores missing differentiated cell and organismic characteristics. Symbiogenesis as a source of metazoan species has consequences for concepts of development, from the origins of cell lines to the evolution of differentiation. PMID:8100457

  6. Benthic Community Composition and Seabed Characteristics of a Chukchi Sea Pockmark

    NASA Astrophysics Data System (ADS)

    MacDonald, I. R.; Bluhm, B.; Iken, K.; Gagaev, S.; Robinson, S.

    2005-12-01

    Several dozen seafloor features were mapped by Larry Mayer and his colleagues using swath bathymetry during a 2003 cruise with the USCGC HEALY near the eastern edge of the Chukchi Plateau (Chukchi Sea 76.6N, 163.9W). These were sub-circular depressions ranging from approximately 250 to over 1000m in width, with depths of up to 50m below the surrounding seabed, and situated in water depths from 500 to 950m. The origin of these features was undetermined, but one possibility was that they were pockmarks formed as a result of gas or fluid expulsion processes. We report here on benthic sampling undertaken at one of these pockmarks on 18 July 2005, also from USCGC HEALY. This elongated feature had maximum water depth of approximately 940m, was 1200m in maximum width, and was depressed approximately 40m below the surrounding seabed. The ocean in the vicinity of the pockmark was heavily ice-covered, which tightly restricted the ship's mobility during sampling operations. We used an ROV to collect and photograph the benthic epifauna during a 6h transit that crossed from the outside of the pockmark to near the center over a distance of 900m. We used a down-looking digital camera to collect over 800 pictures of the benthos at altitudes of 2 to 3m above the seabed. We also collected three cores with a 25x25cm box corer. Our investigations did not provide any direct evidence for gas or fluid flux through the seabed of this feature. Neither did we see any secondary indications of methane flux such as authigenic carbonates or bacterial mats. The abundance and diversity of benthic epifauna at this station was the highest among 8 stations sampled using similar methods during a 30 day cruise. The ROV observed brittle stars, various types of anemones, shrimps, eel pouts, stalked crinoids, benthic ctenophore (likely new species), burrows and mounts, gooseneck barnacles, mysids. Holothurians (c.f. Peneagone sp.) were the single most abundant group and were often photographed in densities of over 50 individuals per square meter. Preliminary analysis of the box core samples: Polychaetes (e.g. Chaetozone setose, Aricidea sp., Ophelina sp., Progoniada sp., Proclea graffi, Protula globifera), Foraminifera, Nemertini, Coronata (Cnidaria tubes), Sipunculida (Golfingia), Bivalvia, Anthozoa.

  7. Chromospheric anemone jets and magnetic reconnection in partially ionized solar atmosphere

    SciTech Connect

    Singh, K. A. P.; Shibata, K.; Nishizuka, N.; Isobe, H.

    2011-11-15

    The solar optical telescope onboard Hinode with temporal resolution of less than 5 s and spatial resolution of 150 km has observed the lower solar atmosphere with an unprecedented detail. This has led to many important findings, one of them is the discovery of chromospheric anemone jets in the solar chromosphere. The chromospheric anemone jets are ubiquitous in solar chromosphere and statistical studies show that the typical length, life time and energy of the chromospheric anemone jets are much smaller than the coronal events (e.g., jets/flares/CMEs). Among various observational parameters, the apparent length and maximum velocity shows good correlation. The velocity of chromospheric anemone jets is comparable to the local Alfven speed in the lower solar chromosphere. Since the discovery of chromospheric anemone jets by Hinode, several evidences of magnetic reconnection in chromospheric anemone jets have been found and these observations are summarized in this paper. These observations clearly suggest that reconnection occurs quite rapidly as well as intermittently in the solar chromosphere. In the solar corona ({lambda}{sub i} > {delta}{sub SP}), anomalous resistivity arises due to various collisionless processes. Previous MHD simulations show that reconnection becomes fast as well as strongly time-dependent due to anomalous resistivity. Such processes would not arise in the solar chromosphere which is fully collisional and partially-ionized. So, it is unclear how the rapid and strongly time-dependent reconnection would occur in the solar chromosphere. It is quite likely that the Hall and ambipolar diffusion are present in the solar chromosphere and they could play an important role in driving such rapid, strongly time-dependent reconnection in the solar chromosphere.

  8. Agent of whirling disease meets orphan worm: phylogenomic analyses firmly place Myxozoa in Cnidaria.

    PubMed

    Nesnidal, Maximilian P; Helmkampf, Martin; Bruchhaus, Iris; El-Matbouli, Mansour; Hausdorf, Bernhard

    2013-01-01

    Myxozoa are microscopic obligate endoparasites with complex live cycles. Representatives are Myxobolus cerebralis, the causative agent of whirling disease in salmonids, and the enigmatic "orphan worm" Buddenbrockia plumatellae parasitizing in Bryozoa. Originally, Myxozoa were classified as protists, but later several metazoan characteristics were reported. However, their phylogenetic relationships remained doubtful. Some molecular phylogenetic analyses placed them as sister group to or even within Bilateria, whereas the possession of polar capsules that are similar to nematocysts of Cnidaria and of minicollagen genes suggest a close relationship between Myxozoa and Cnidaria. EST data of Buddenbrockia also indicated a cnidarian origin of Myxozoa, but were not sufficient to reject a closer relationship to bilaterians. Phylogenomic analyses of new genomic sequences of Myxobolus cerebralis firmly place Myxozoa as sister group to Medusozoa within Cnidaria. Based on the new dataset, the alternative hypothesis that Myxozoa form a clade with Bilateria can be rejected using topology tests. Sensitivity analyses indicate that this result is not affected by long branch attraction artifacts or compositional bias. PMID:23382916

  9. NUMERICAL SIMULATIONS OF CHROMOSPHERIC ANEMONE JETS ASSOCIATED WITH MOVING MAGNETIC FEATURES

    SciTech Connect

    Yang, Liping; He, Jiansen; Tu, Chuanyi; Zhang, Lei; Peter, Hardi; Feng, Xueshang; Zhang, Shaohua

    2013-11-01

    Observations with the space-based solar observatory Hinode show that small-scale magnetic structures in the photosphere are found to be associated with a particular class of jets of plasma in the chromosphere called anemone jets. The goal of our study is to conduct a numerical experiment of such chromospheric anemone jets related to the moving magnetic features (MMFs). We construct a 2.5 dimensional numerical MHD model to describe the process of magnetic reconnection between the MMFs and the pre-existing ambient magnetic field, which is driven by the horizontal motion of the magnetic structure in the photosphere. We include thermal conduction parallel to the magnetic field and optically thin radiative losses in the corona to account for a self-consistent description of the evaporation process during the heating of the plasma due to the reconnection process. The motion of the MMFs leads to the expected jet and our numerical results can reproduce many observed characteristics of chromospheric anemone jets, topologically and quantitatively. As a result of the tearing instability, plasmoids are generated in the reconnection process that are consistent with the observed bright moving blobs in the anemone jets. An increase in the thermal pressure at the base of the jet is also driven by the reconnection, which induces a train of slow-mode shocks propagating upward. These shocks are a secondary effect, and only modulate the outflow of the anemone jet. The jet itself is driven by the energy input due to the reconnection of the MMFs and the ambient magnetic field.

  10. Developing the anemone Aiptasia as a tractable model for cnidarian-dinoflagellate symbiosis: the transcriptome of aposymbiotic A. pallida

    PubMed Central

    2012-01-01

    Background Coral reefs are hotspots of oceanic biodiversity, forming the foundation of ecosystems that are important both ecologically and for their direct practical impacts on humans. Corals are declining globally due to a number of stressors, including rising sea-surface temperatures and pollution; such stresses can lead to a breakdown of the essential symbiotic relationship between the coral host and its endosymbiotic dinoflagellates, a process known as coral bleaching. Although the environmental stresses causing this breakdown are largely known, the cellular mechanisms of symbiosis establishment, maintenance, and breakdown are still largely obscure. Investigating the symbiosis using an experimentally tractable model organism, such as the small sea anemone Aiptasia, should improve our understanding of exactly how the environmental stressors affect coral survival and growth. Results We assembled the transcriptome of a clonal population of adult, aposymbiotic (dinoflagellate-free) Aiptasia pallida from ~208 million reads, yielding 58,018 contigs. We demonstrated that many of these contigs represent full-length or near-full-length transcripts that encode proteins similar to those from a diverse array of pathways in other organisms, including various metabolic enzymes, cytoskeletal proteins, and neuropeptide precursors. The contigs were annotated by sequence similarity, assigned GO terms, and scanned for conserved protein domains. We analyzed the frequency and types of single-nucleotide variants and estimated the size of the Aiptasia genome to be ~421?Mb. The contigs and annotations are available through NCBI (Transcription Shotgun Assembly database, accession numbers JV077153-JV134524) and at http://pringlelab.stanford.edu/projects.html. Conclusions The availability of an extensive transcriptome assembly for A. pallida will facilitate analyses of gene-expression changes, identification of proteins of interest, and other studies in this important emerging model system. PMID:22726260

  11. Development and epithelial organisation of muscle cells in the sea anemone Nematostella vectensis

    PubMed Central

    2014-01-01

    Introduction Nematostella vectensis, a member of the cnidarian class Anthozoa, has been established as a promising model system in developmental biology, but while information about the genetic regulation of embryonic development is rapidly increasing, little is known about the cellular organization of the various cell types in the adult. Here, we studied the anatomy and development of the muscular system of N. vectensis to obtain further insights into the evolution of muscle cells. Results The muscular system of N. vectensis is comprised of five distinct muscle groups, which are differentiated into a tentacle and a body column system. Both systems house longitudinal as well as circular portions. With the exception of the ectodermal tentacle longitudinal muscle, all muscle groups are of endodermal origin. The shape and epithelial organization of muscle cells vary considerably between different muscle groups. Ring muscle cells are formed as epitheliomuscular cells in which the myofilaments are housed in the basal part of the cell, while the apical part is connected to neighboring cells by apical cell-cell junctions. In the longitudinal muscles of the column, the muscular part at the basal side is connected to the apical part by a long and narrow cytoplasmic bridge. The organization of these cells, however, remains epitheliomuscular. A third type of muscle cell is represented in the longitudinal muscle of the tentacle. Using transgenic animals we show that the apical cell-cell junctions are lost during differentiation, resulting in a detachment of the muscle cells to a basiepithelial position. These muscle cells are still located within the epithelium and outside of the basal matrix, therefore constituting basiepithelial myocytes. We demonstrate that all muscle cells, including the longitudinal basiepithelial muscle cells of the tentacle, initially differentiate from regular epithelial cells before they alter their epithelial organisation. Conclusions A wide range of different muscle cell morphologies can already be found in a single animal. This suggests how a transition from an epithelially organized muscle system to a mesenchymal could have occurred. Our study on N. vectensis provides new insights into the organisation of a muscle system in a non-bilaterian organism. PMID:25009575

  12. Functional characterisation of a TRPM2 orthologue from the sea anemone Nematostella vectensis in human cells.

    PubMed

    Kühn, Frank J P; Kühn, Cornelia; Lückhoff, Andreas

    2015-01-01

    The human non-selective cation channel TRPM2 represents a mediator of apoptosis triggered by oxidative stress. The principal agonist ADP-ribose binds to the cytosolic domain of TRPM2, which is homologous to the human ADP-ribose pyrophosphatase NUDT9. To further elucidate the structure-function relationship of this channel, we characterised a TRPM2 orthologue from the cnidarian Nematostella vectensis, after its expression in a human cell line. This far distant relative shows only 31% total sequence similarity to hTRPM2, while its C-terminal domain has a greater resemblance to the NUDT9 enzyme. Current through nvTRPM2 was induced by ADPR, with a more pronounced sensitivity and faster kinetics than in hTRPM2. In contrast to hTRPM2, there was no response to H2O2 and hardly any modulatory effect by intracellular Ca(2+). The deletion of a stretch of 15 residues from the NUDT9 domain of nvTRPM2, which is absent in hTRPM2, did not change the response to ADPR but enabled activation of the channel by H2O2 and increased the effects of intracellular Ca(2+). These findings shed new light on the evolution of TRPM2 and establish nvTRPM2 as a promising tool to decipher its complex gating mechanisms. PMID:25620041

  13. Functional Characterisation of a TRPM2 orthologue from the sea anemone Nematostella vectensis in human cells

    PubMed Central

    Kühn, Frank J. P.; Kühn, Cornelia; Lückhoff, Andreas

    2015-01-01

    The human non-selective cation channel TRPM2 represents a mediator of apoptosis triggered by oxidative stress. The principal agonist ADP-ribose binds to the cytosolic domain of TRPM2, which is homologous to the human ADP-ribose pyrophosphatase NUDT9. To further elucidate the structure-function relationship of this channel, we characterised a TRPM2 orthologue from the cnidarian Nematostella vectensis, after its expression in a human cell line. This far distant relative shows only 31% total sequence similarity to hTRPM2, while its C-terminal domain has a greater resemblance to the NUDT9 enzyme. Current through nvTRPM2 was induced by ADPR, with a more pronounced sensitivity and faster kinetics than in hTRPM2. In contrast to hTRPM2, there was no response to H2O2 and hardly any modulatory effect by intracellular Ca2+. The deletion of a stretch of 15 residues from the NUDT9 domain of nvTRPM2, which is absent in hTRPM2, did not change the response to ADPR but enabled activation of the channel by H2O2 and increased the effects of intracellular Ca2+. These findings shed new light on the evolution of TRPM2 and establish nvTRPM2 as a promising tool to decipher its complex gating mechanisms. PMID:25620041

  14. The impact of the Tekay chromoviral elements on genome organisation and evolution of Anemone s.l. (Ranunculaceae).

    PubMed

    Mlinarec, J; Franjevi?, D; Harapin, J; Besendorfer, V

    2016-03-01

    We studied the highly abundant chromoviral Tekay clade in species from three sister genera - Anemone, Pulsatilla and Hepatica (Ranunculaceae). With this clade, we performed a concomitant survey of its phylogenetic diversity, chromosomal organisation and transcriptional activity in Anemone s.l. in order to investigate dynamics of the Tekay elements at a finer scale than previously achieved in this or any other flowering clade. The phylogenetic tree built from Tekay sequences conformed to expected evolutionary relationships of the species; exceptions being A. nemorosa and A. sylvestris, which appeared more closely related that expected, and we invoke hybridisation events to explain the observed topology. The separation of elements into six clusters could be explained by episodic bursts of activity since divergence from a common ancestor at different points in their respective evolutionary histories. In Anemone s.l. the Tekay elements do not have a preferential position on chromosomes, i.e. they can have a: (i) centromeric/pericentromeric position; (ii) interstitial position in DAPI-positive AT-rich heterochromatic regions; can be (iii) dispersed throughout chromosomes; or even (iv) be absent from large heterochromatic blocks. Widespread transcriptional activity of the Tekay elements in Anemone s.l. taxa indicate that some copies of Tekay elements could still be active in this plant group, contributing to genome evolution and speciation within Anemone s.l. Identification of Tekay elements in Anemone s.l. provides valuable information for understanding how different localisation patterns might help to facilitate plant genome organisation in a structural and functional manner. PMID:26370195

  15. MULTIPLE PLASMA EJECTIONS AND INTERMITTENT NATURE OF MAGNETIC RECONNECTION IN SOLAR CHROMOSPHERIC ANEMONE JETS

    SciTech Connect

    Singh, K. A. P.; Nishida, K.; Shibata, K.; Isobe, H.; Nishizuka, N. E-mail: nishida@kwasan.kyoto-u.ac.jp E-mail: isobe@kwasan.kyoto-u.ac.jp

    2012-11-01

    The recent discovery of chromospheric anemone jets with the Solar Optical Telescope (SOT) on board Hinode has shown an indirect evidence of magnetic reconnection in the solar chromosphere. However, the basic nature of magnetic reconnection in chromosphere is still unclear. We studied nine chromospheric anemone jets from SOT/Hinode using Ca II H filtergrams, and we found multiple bright, plasma ejections along the jets. In most cases, the major intensity enhancements (larger than 30% relative to the background intensity) of the loop correspond to the timing of the plasma ejections. The typical lifetime and size of the plasma ejecta are about 20-60 s and 0.3-1.5 Mm, respectively. The height-time plot of jet shows many sub-structures (or individual jets) and the typical lifetime of the individual jet is about one to five minutes. Before the onset of the jet activity, a loop appears in Ca II H and gradually increases in size, and after few minutes several jets are launched from the loop. Once the jet activity starts and several individual jets are launched, the loop starts shrinking with a speed of {approx}4 km s{sup -1}. In some events, a downward moving blob with a speed of {approx}35 km s{sup -1} was observed, associated with the upward moving plasma along one of the legs of the loop hosting the jets. The upward moving plasma gradually developed into jets. Multiple plasma ejections in chromospheric anemone jet show the strongly time-dependent as well as intermittent nature of magnetic reconnection in the solar chromosphere.

  16. Active nematocyst isolation via nudibranchs.

    PubMed

    Schlesinger, Ami; Kramarsky-Winter, Esti; Loya, Yossi

    2009-01-01

    Cnidarian venoms are potentially valuable tools for biomedical research and drug development. They are contained within nematocysts, the stinging organelles of cnidarians. Several methods exist for the isolation of nematocysts from cnidarian tissues; most are tedious and target nematocysts from specific tissues. We have discovered that the isolated active nematocyst complement (cnidome) of several sea anemone (Cnidaria: Anthozoa) species is readily accessible. These nematocysts are isolated, concentrated, and released to the aqueous environment as a by-product of aeolid nudibranch Spurilla neapolitana cultures. S. neapolitana feed on venomous sea anemones laden with stinging nematocysts. The ingested stinging organelles of several sea anemone species are effectively excreted in the nudibranch feces. We succeeded in purifying the active organelles and inducing their discharge. Thus, our current study presents the attractive possibility of using nudibranchs to produce nematocysts for the investigation of novel marine compounds. PMID:19184220

  17. Global Diversity and Review of Siphonophorae (Cnidaria: Hydrozoa)

    PubMed Central

    Mapstone, Gillian M.

    2014-01-01

    In this review the history of discovery of siphonophores, from the first formal description by Carl Linnaeus in 1785 to the present, is summarized, and species richness together with a summary of world-wide distribution of this pelagic group within the clade Hydrozoa discussed. Siphonophores exhibit three basic body plans which are briefly explained and figured, whilst other atypical body plans are also noted. Currently, 175 valid siphonophore species are recognized in the latest WoRMS world list, including 16 families and 65 genera. Much new information since the last review in 1987 is revealed from the first molecular analysis of the group, enabling identification of some new morphological characters diagnostic for physonect siphonophores. Ten types of nematocysts (stinging cells) are identified in siphonophores, more than in any other cnidarian; these are incorporated into batteries in the side branches of the tentacles in most species (here termed tentilla), and tentilla are reviewed in the last section of this paper. Their discharge mechanisms are explained and also how the tentilla of several physonect siphonophores are modified into lures. Of particular interest is the recent discovery of a previously unknown red fluorescent lure in the tentilla of the deep sea physonect Erenna, the first described example of emission of red light by an invertebrate to attract prey. PMID:24516560

  18. Global diversity and review of Siphonophorae (Cnidaria: Hydrozoa).

    PubMed

    Mapstone, Gillian M

    2014-01-01

    In this review the history of discovery of siphonophores, from the first formal description by Carl Linnaeus in 1785 to the present, is summarized, and species richness together with a summary of world-wide distribution of this pelagic group within the clade Hydrozoa discussed. Siphonophores exhibit three basic body plans which are briefly explained and figured, whilst other atypical body plans are also noted. Currently, 175 valid siphonophore species are recognized in the latest WoRMS world list, including 16 families and 65 genera. Much new information since the last review in 1987 is revealed from the first molecular analysis of the group, enabling identification of some new morphological characters diagnostic for physonect siphonophores. Ten types of nematocysts (stinging cells) are identified in siphonophores, more than in any other cnidarian; these are incorporated into batteries in the side branches of the tentacles in most species (here termed tentilla), and tentilla are reviewed in the last section of this paper. Their discharge mechanisms are explained and also how the tentilla of several physonect siphonophores are modified into lures. Of particular interest is the recent discovery of a previously unknown red fluorescent lure in the tentilla of the deep sea physonect Erenna, the first described example of emission of red light by an invertebrate to attract prey. PMID:24516560

  19. New records of Primnoidae (Cnidaria: Octocorallia) in Brazilian deep waters

    NASA Astrophysics Data System (ADS)

    Arantes, Renata C. M.; Loiola, Livia L.

    2014-01-01

    The knowledge of octocorals occurring in Brazilian deep waters is still lacking, with only a few studies conducted so far, most of which focused on large-scale marine habitats characterization. Primnoidae are common and characteristic of seamounts and deepwater coral banks, often providing habitat for other marine species. Although primnoids occur in all ocean basins, only Primnoella and Plumarella species were recorded along the Brazilian coast before this study. Primnoid specimens were obtained through dredging and remotely operated vehicles (ROV) sampling, collected by research projects conducted off the Brazilian coast, between 15 and 34°S. Taxonomic assessment resulted in 5 new records of Primnoidae genera in Brazil: Calyptrophora, Candidella, Dasystenella, Narella and Thouarella. The occurrences of Narella-off Salvador and Vitória, and in Campos Basin (935-1700 m), and Calyptrophora-in Campos Basin (1059-1152 m), are herein reported for the first time in the South Atlantic. Calyptrophora microdentata was previously known in Lesser Antilles, New England and Corner Rise Seamounts, between 686 and 2310 m. Candidella imbricata geographical distribution includes Western and Eastern Atlantic (514-2063 m and 815-2139 m, respectively), being registered herein in Campos Basin, between 1059 and 1605 m. Dasystenella acanthina collected off Rio Grande do Sul state (810 m) and occurs also off Argentina and Southern Ocean, between 150 and 5087 m. Plumarella diadema, which type locality is off São Sebastião, Brazil, has its geographical range extended northwards, occurring in Campos Basin (650 m). Thouarella koellikeri previously known for Patagonia and Antartic Peninsula, is registered for the off Brazil for the first time, in Campos Basin and off São Sebastião (609-659 m). There is a lot of work yet to be done in terms of taxonomic knowledge of Brazilian deep-sea octocorals. Research projects focusing on the investigations, including ROV sampling, of other geographical regions and depth ranges along Brazilian coast will certainly reveal other new octocorals occurrences and species.

  20. Isolation and identification of chitin in the black coral Parantipathes larix (Anthozoa: Cnidaria).

    PubMed

    Bo, Marzia; Bavestrello, Giorgio; Kurek, Denis; Paasch, Silvia; Brunner, Eike; Born, René; Galli, Roberta; Stelling, Allison L; Sivkov, Viktor N; Petrova, Olga V; Vyalikh, Denis; Kummer, Kurt; Molodtsov, Serguei L; Nowak, Dorota; Nowak, Jakub; Ehrlich, Hermann

    2012-01-01

    Until now, there is a lack of knowledge about the presence of chitin in numerous representatives of corals (Cnidaria). However, investigations concerning the chitin-based skeletal organization in different coral taxa are significant from biochemical, structural, developmental, ecological and evolutionary points of view. In this paper, we present a thorough screening for the presence of chitin within the skeletal formations of a poorly investigated Mediterranean black coral, Parantipathes larix (Esper, 1792), as a typical representative of the Schizopathidae family. Using a wide array variety of techniques ((13)C solid state NMR, Fourier transform infrared (FTIR), Raman, NEXAFS, Morgan-Elson assay and Calcofluor White Staining), we unambiguously show for the first time that chitin is an important component within the skeletal stalks as well as pinnules of this coral. PMID:22546360

  1. Phylogenetic placement of the enigmatic parasite, Polypodium hydriforme, within the Phylum Cnidaria

    PubMed Central

    2008-01-01

    Background Polypodium hydriforme is a parasite with an unusual life cycle and peculiar morphology, both of which have made its systematic position uncertain. Polypodium has traditionally been considered a cnidarian because it possesses nematocysts, the stinging structures characteristic of this phylum. However, recent molecular phylogenetic studies using 18S rDNA sequence data have challenged this interpretation, and have shown that Polypodium is a close relative to myxozoans and together they share a closer affinity to bilaterians than cnidarians. Due to the variable rates of 18S rDNA sequences, these results have been suggested to be an artifact of long-branch attraction (LBA). A recent study, using multiple protein coding markers, shows that the myxozoan Buddenbrockia, is nested within cnidarians. Polypodium was not included in this study. To further investigate the phylogenetic placement of Polypodium, we have performed phylogenetic analyses of metazoans with 18S and partial 28S rDNA sequences in a large dataset that includes Polypodium and a comprehensive sampling of cnidarian taxa. Results Analyses of a combined dataset of 18S and partial 28S sequences, and partial 28S alone, support the placement of Polypodium within Cnidaria. Removal of the long-branched myxozoans from the 18S dataset also results in Polypodium being nested within Cnidaria. These results suggest that previous reports showing that Polypodium and Myxozoa form a sister group to Bilateria were an artifact of long-branch attraction. Conclusion By including 28S rDNA sequences and a comprehensive sampling of cnidarian taxa, we demonstrate that previously conflicting hypotheses concerning the phylogenetic placement of Polypodium can be reconciled. Specifically, the data presented provide evidence that Polypodium is indeed a cnidarian and is either the sister taxon to Hydrozoa, or part of the hydrozoan clade, Leptothecata. The former hypothesis is consistent with the traditional view that Polypodium should be placed in its own cnidarian class, Polypodiozoa. PMID:18471296

  2. Phylogenetic analysis reveals an evolutionary transition from internal to external brooding in Epiactis Verrill (Cnidaria: Anthozoa: Actiniaria) and rejects the validity of the genus Cnidopus Carlgren.

    PubMed

    Larson, Paul G; Daly, Marymegan

    2016-01-01

    Reproductive behaviors in the sea anemone genus Epiactis provide an opportunity for investigating the evolution of reproductive phenomena such as brooding and sex allocation (hermaphroditic vs. gonochoric) in a group of closely related and easily accessible species. However, given its broad geographic distribution, the striking diversity in reproductive behaviors, and the lack of synapomorphy for the genus, the monophyly of Epiactis is questionable. Here we perform phylogenetic analyses to test the monophyly of Epiactis and the validity of Cnidopus, which consists entirely of species once assigned to Epiactis. We use the large number of brooding species in Epiactis to investigate evolutionary patterns in brooding modes and characteristics associated with them. We find a monophyletic group of North Pacific Epiactis species: this group includes the type species of the genus and species that brood internally or externally, and that are hermaphroditic or gonochoric. Based on the results, we reject the genus Cnidopus because its circumscription renders Epiactis sensu stricto paraphyletic. Ancestral character state reconstruction indicates that in the North Pacific, externally brooding species evolved from internally brooding ancestors and that sex allocation is highly labile. Species relationships in Epiactis and Aulactinia appear to conform to geographic patterns more strongly than to taxonomic hypotheses. Contrary to expectations based on other invertebrates, we fail to find a strong correlation between brooding and hermaphroditism. PMID:26477737

  3. Dangerous reef aquaristics: Palytoxin of a brown encrusting anemone causes toxic corneal reactions.

    PubMed

    Ruiz, Yasmin; Fuchs, Joan; Beuschel, Ralf; Tschopp, Markus; Goldblum, David

    2015-11-01

    Although frequently observed in domestic saltwater aquariums, literature on exposure to palytoxin (PTX) of encrusting anemones (Zoanthidea) kept in aquariums is rare. Handling these animals for propagation purposes or during cleaning work can lead to dermal, ocular or respiratory contact with the PTX generated by some Zoanthids. The present study describes a case of ocular exposure to liquid from a Zoanthid, which led to corneal ulcers. The patient also suffered from systemic symptoms of dyspnea and shivering and a suspected rhabdomyolysis, which required monitoring in the Intensive Care Unit. After symptomatic treatment provided insufficient results, the corneal ulcers improved with an amniotic membrane transplantation. A review of the literature regarding ocular exposures to this diverse order of Hexacorallia reveals that severe and systemic symptoms can develop with minimal contact. PMID:26365918

  4. Shallow-water zoantharians (Cnidaria, Hexacorallia) from the Central Indo-Pacific

    PubMed Central

    Reimer, James D.; Poliseno, Angelo; Hoeksema, Bert W.

    2014-01-01

    Abstract Despite the Central Indo-Pacific (CIP) and the Indonesian Archipelago being a well-known region of coral reef biodiversity, particularly in the ‘Coral Triangle’, little published information is available on its zoantharians (Cnidaria: Hexacorallia: Zoantharia). In order to provide a basis for future research on the Indo-Pacific zoantharian fauna and facilitate comparisons between more well-studied regions such as Japan and the Great Barrier Reef, this report deals with CIP zoantharian specimens in the Naturalis collection in Leiden, the Netherlands; 106 specimens were placed into 24 morpho-species and were supplemented with 88 in situ photographic records from Indonesia, the Philippines, and Papua New Guinea. At least nine morpho-species are likely to be undescribed species, indicating that the region needs more research in order to properly understand zoantharian diversity within the CIP. The Naturalis’ zoantharian specimens are listed by species, as well as all relevant collection information, and in situ images are provided to aid in future studies on zoantharians in the CIP. PMID:25349499

  5. Evolution of box jellyfish (Cnidaria: Cubozoa), a group of highly toxic invertebrates

    PubMed Central

    Bentlage, Bastian; Cartwright, Paulyn; Yanagihara, Angel A.; Lewis, Cheryl; Richards, Gemma S.; Collins, Allen G.

    2010-01-01

    Cubozoa (Cnidaria: Medusozoa) represents a small clade of approximately 50 described species, some of which cause serious human envenomations. Our understanding of the evolutionary history of Cubozoa has been limited by the lack of a sound phylogenetic hypothesis for the group. Here, we present a comprehensive cubozoan phylogeny based on ribosomal genes coding for near-complete nuclear 18S (small subunit) and 28S (large subunit) and partial mitochondrial 16S. We discuss the implications of this phylogeny for our understanding of cubozoan venom evolution, biogeography and life-history evolution. Our phylogenetic hypothesis suggests that: (i) the last common ancestor of Carybdeida probably possessed the mechanism(s) underlying Irukandji syndrome, (ii) deep divergences between Atlantic and Indo-Pacific clades may be explained by ancient vicariant events, and (iii) sexual dimorphism evolved a single time in concert with complex sexual behaviour. Furthermore, several cubozoan taxa are either para- or polyphyletic, and we address some of these taxonomic issues by designating a new family, Carukiidae, a new genus, Copula, and by redefining the families Tamoyidae and Tripedaliidae. Lastly, cubozoan species identities have long been misunderstood and the data presented here support many of the recent scientific descriptions of cubozoan species. However, the results of a phylogeographic analysis of Alatina moseri from Hawai'i and Alatina mordens from Australia indicate that these two nominal species represent a single species that has maintained metapopulation cohesion by natural or anthropogenic dispersal. PMID:19923131

  6. Gross and microscopic morphology of lesions in Cnidaria from Palmyra Atoll, Central Pacific

    USGS Publications Warehouse

    Williams, Gareth J.; Work, Thierry M.; Aeby, Greta S.; Knapp, Ingrid S.; Davy, Simon K.

    2011-01-01

    We conducted gross and microscopic characterizations of lesions in Cnidaria from Palmyra Atoll, Central Pacific. We found growth anomalies (GA) to be the most commonly encountered lesion. Cases of discoloration and tissue loss were rare. GAs had a focal or multi-focal distribution and were predominantly nodular, exophytic, and umbonate. In scleractinians, the majority of GAs manifested as hyperplasia of the basal body wall (52% of cases), with an associated absence or reduction of polyp structure (mesenteries and filaments, actinopharynx and tentacles), and depletion of zooxanthellae in the gastrodermis of the upper body wall. In the soft corals Sinularia sp. and Lobophytum sp., GAs exclusively manifested as prominent hyperplasia of the coenenchyme with an increased density of solenia. In contrast to scleractinians, soft coral GAs displayed an inflammatory and necrotizing component with marked edema of the mesoglea, accompanied by infiltrates of variably-sized granular amoebocytes. Fungi, algae, sponges, and Crustacea were present in some scleractinian GAs, but absent in soft coral GAs. Fragmentation of tissues was a common finding in Acropora acuminata and Montipora cf. dilatata colonies with tissue loss, although no obvious causative agents were seen. Discoloration in the zoanthid, Palythoa tuberculosa, was found to be the result of necrosis, while in Lobophytum sp. discoloration was the result of zooxanthellar depletion (bleaching). Soft corals with discoloration or tissue loss showed a marked inflammatory response, however no obvious causative organisms were seen. Lesions that appeared similar at the gross level were revealed to be distinct by microscopy, emphasizing the importance of histopathology.

  7. A new species of antipatharian coral (Cnidaria: Anthozoa: Antipatharia: Schizopathidae) from the Pacific coast of Costa Rica

    SciTech Connect

    Opresko, Dennis M; Breedy, Odalisca

    2010-09-01

    A new species of black coral, Aphanipathes colombiana (Cnidaria:Antipatharia) from the Caribbean coast of Colombia is described. The species forms small flabellate colonies with anisomorphic polypar spines. It is morphologically similar to the western Atlantic species A. thyoides (Pourtales) but its hypostomal polypar spines are not reduced in size. The new species also resembles the Indo-Pacific species A. reticulata van Pesch but it has smooth-surfaced polypar spines, whereas in A. reticulata these spines have small tubercles on their surface

  8. Observations of Chromospheric Anemone Jets with Hinode CaII Broadband Filtergraph and Hida CaII Spectroheliograph

    NASA Astrophysics Data System (ADS)

    Morita, Satoshi; Shibata, Kazunari; Ueno, Satoru; Ichimoto, Kiyoshi; Kitai, Reizaburo; Otsuji, Ken-Ichi

    2010-08-01

    We report on the first simultaneous observations of chromospheric ``anemone'' jets in solar active regions with the Hinode Solar Optical Telescope (SOT) CaII H broadband filtergraph and the CaII K spetroheliograph on the Domeless Solar Telescope (DST) at Hida Observatory. During the period of coordinated observations, nine chromospheric anemone jets were simultaneously observed with the two instruments. These observations revealed three important features: (1) the jets are generated in the lower chromosphere; i.e., these cannot be seen in CaII K3; (2) the length and lifetime of the jets are 0.4-5 Mm and 40-320 s, respectively; (3) the apparent velocity of the jets observed with the SOT is 3-24 km s-1, while the CaII K3 component at the jets shows a blueshift (in 5 events) in the range of 2-6 km s-1. The chromospheric anemone jets are associated with mixed polarity regions, which are either small emerging flux regions or moving magnetic features. It is found that the CaII K line often shows red or blue asymmetry in the K2/K1 component; the footpoint of the jets associated with emerging flux regions often shows a redshift (2-16 km s-1), while the one with moving magnetic features shows a blueshift (˜5 km s-1). A detailed analysis of the magnetic evolution of the jet-forming regions revealed that the reconnection rate (or canceling rate) of the total magnetic flux at the footpoint of the jets is on the order of 1016 Mx s-1, and the resulting magnetic energy release rate is (1.1-10) × 1024 erg s-1, with a total energy release of (1-13) × 1026 erg for the duration of the magnetic cancellation, ˜130 s. These are comparable to the estimated total energy, ˜1026 erg, in a single chromospheric anemone jet. In addition to the DST CaII K spectroheliogram and the SOT CaII H broadband filtergram, we also used for analysis an SOT magnetogram as well as a Hida H? filtergram. We present a physical model of the jet based on the observation, and discuss the relation between chromospheric anemone jets and Ellerman bombs.

  9. Evolution of Hox-like genes in Cnidaria: Study of Hydra Hox repertoire reveals tailor-made Hox-code for Cnidarians.

    PubMed

    Reddy, Puli Chandramouli; Unni, Manu K; Gungi, Akhila; Agarwal, Pallavi; Galande, Sanjeev

    2015-11-01

    Hox and ParaHox genes play decisive roles in patterning the anterior-posterior body axis in Bilateria. Evolutionary origin of Hox genes and primary body axis predate the divergence of Bilateria and Cnidaria. However, function of Cnidarian Hox-like genes and their regulation in axis determination is obscure due to studies limited to a few representative model systems. Present investigation is conducted using Hydra, a Hydrozoan member of phylum Cnidaria, to gain insights into the roles of Cnidarian Hox-like genes in primary axis formation. Here, we report identification of six Hox-like genes from our in-house transcriptome data. Phylogenetic analysis of these genes shows bilaterian counterparts of Hox1, Gsx and Mox. Additionally, we report CnoxB_HVUL, CnoxC2_HVUL and CnoxC3_HVUL belonging to two Cnidarian specific groups. In situ hybridization analysis of Hydra homologues provided important clues about their possible roles in pattern formation of polyps and bud development. Specifically, Hox1_HVUL is regulated by Wnt signaling and plays critical role in head formation. Collating information about expression patterns of different Hox-like genes from previous reports and this study reveals no conformity within Cnidaria. Indicating that unlike in Bilateria, there is no consolidated Hox-code determining primary body axis in Cnidaria. PMID:26278345

  10. Molecular cloning and characterization of a steroidogenic enzyme, 17β-hydroxysteroid dehydrogenase type 14, from the stony coral Euphyllia ancora (Cnidaria, Anthozoa).

    PubMed

    Shikina, Shinya; Chung, Yi-Jou; Chiu, Yi-Ling; Huang, Yi-Jie; Lee, Yan-Horn; Chang, Ching-Fong

    2016-03-01

    Sex steroids play a fundamental role not only in reproduction but also in various other biological processes in vertebrates. Although the presence of sex steroids has been confirmed in cnidarians (e.g., coral, sea anemone, jellyfish, and hydra), which are basal metazoans, only a few studies to date have characterized steroidogenesis-related genes in cnidarians. Based on a transcriptomic analysis of the stony coral Euphyllia ancora, we identified the steroidogenic enzyme 17β-hydroxysteroid dehydrogenase type 14 (17beta-hsd 14), an oxidative enzyme that catalyzes the NAD(+)-dependent inactivation of estrogen/androgen (estradiol to estrone and testosterone to androstenedione) in mammals. Phylogenetic analysis showed that E. ancora 17beta-Hsd 14 (Ea17beta-Hsd 14) clusters with other animal 17beta-HSD 14s but not with other members of the 17beta-HSD family. Subsequent quantitative RT-PCR analysis revealed a lack of correlation of Ea17beta-hsd 14 transcript levels with the coral's reproductive cycle. In addition, Ea17beta-hsd 14 transcript and protein were detected in all tissues examined, such as the tentacles, mesenterial filaments, and gonads, at similar levels in both sexes, as determined by quantitative RT-PCR analysis and Western blotting with an anti-Ea17beta-Hsd 14 antibody. Immunohistochemical analysis revealed that Ea17beta-Hsd 14 is mainly distributed in the endodermal regions of the polyps, but the protein was also observed in all tissues examined. These results suggest that Ea17beta-Hsd 14 is involved in important functions that commonly occur in endodermal cells or has multiple functions in different tissues. Our data provide information for comparison with advanced animals as well as insight into the evolution of steroidogenesis-related genes in metazoans. PMID:26868454

  11. Hypothesis--origin of parietal cells: transfer of the H+K+-ATPase gene from parasitic microorganisms to Cnidaria?

    PubMed

    Okabe, S

    1999-09-30

    Parietal cells present in the stomach and terminal ileum secrete a highly-concentrated hydrochloric acid into the lumen. The cells are characterized by the enzyme P-type H+K+-ATPase, which has an alpha-subunit with a high homology (>85%) for the amino acid sequences of frog, mouse and pig stomachs. Gastric H+K+-ATPase also exhibits a high homology to H+-ATPase in yeast and Na+K+-ATPase in many tissues, suggesting origination from a common ancestral ATPase. It is known that parietal cells first appeared in fish and were later expressed in evolutionarily-higher organisms. Primitive organisms, such as Cnidaria and Ctenophora, that possessed digestive organs, but not parietal cells, were abundant in the ocean more than 600 million years ago (Pre-Cambrian period). The author thus hypothesized that the genes of either H+-ATPase or H+K+-ATPase that were present in parasitic microorganisms, such as yeast, were transferred to the interstitial cells of host organisms, such as Cnidaria, eventually leading to the evolution of parietal cells. It appears that although parietal cells in the stomach developed by chance, such cells have greatly contributed to the evolution of advanced organisms, including humans, by affording safe ingestion of a large volume of various foods. PMID:10707885

  12. A latitudinal gradient in seed nutrients of the forest herb Anemone nemorosa.

    PubMed

    De Frenne, P; Kolb, A; Graae, B J; Decocq, G; Baltora, S; De Schrijver, A; Brunet, J; Chabrerie, O; Cousins, S A O; Dhondt, R; Diekmann, M; Gruwez, R; Heinken, T; Hermy, M; Liira, J; Saguez, R; Shevtsova, A; Baskin, C C; Verheyen, K

    2011-05-01

    The nutrient concentration in seeds determines many aspects of potential success of the sexual reproductive phase of plants, including the seed predation probability, efficiency of seed dispersal and seedling performance. Despite considerable research interest in latitudinal gradients of foliar nutrients, a similar gradient for seeds remains unexplored. We investigated a potential latitudinal gradient in seed nutrient concentrations within the widespread European understorey forest herb Anemone nemorosa L. We sampled seeds of A. nemorosa in 15 populations along a 1900-km long latitudinal gradient at three to seven seed collection dates post-anthesis and investigated the relative effects of growing degree-hours >5 °C, soil characteristics and latitude on seed nutrient concentrations. Seed nitrogen, nitrogen:phosphorus ratio and calcium concentration decreased towards northern latitudes, while carbon:nitrogen ratios increased. When taking differences in growing degree-hours and measured soil characteristics into account and only considering the most mature seeds, the latitudinal decline remained particularly significant for seed nitrogen concentration. We argue that the decline in seed nitrogen concentration can be attributed to northward decreasing seed provisioning due to lower soil nitrogen availability or greater investment in clonal reproduction. This pattern may have large implications for the reproductive performance of this forest herb as the degree of seed provisioning ultimately co-determines seedling survival and reproductive success. PMID:21489100

  13. Recent advances in deep-sea natural products.

    PubMed

    Skropeta, Danielle; Wei, Liangqian

    2014-08-01

    Covering: 2009 to 2013. This review covers the 188 novel marine natural products described since 2008, from deep-water (50->5000 m) marine fauna including bryozoa, chordata, cnidaria, echinodermata, microorganisms, mollusca and porifera. The structures of the new compounds and details of the source organism, depth of collection and country of origin are presented, along with any relevant biological activities of the metabolites. Where reported, synthetic studies on the deep-sea natural products have also been included. Most strikingly, 75% of the compounds were reported to possess bioactivity, with almost half exhibiting low micromolar cytotoxicity towards a range of human cancer cell lines, along with a significant increase in the number of microbial deep-sea natural products reported. PMID:24871201

  14. Biodiversity of the white coral bank off Cape Santa Maria di Leuca (Mediterranean Sea): An update

    NASA Astrophysics Data System (ADS)

    Mastrototaro, F.; D'Onghia, G.; Corriero, G.; Matarrese, A.; Maiorano, P.; Panetta, P.; Gherardi, M.; Longo, C.; Rosso, A.; Sciuto, F.; Sanfilippo, R.; Gravili, C.; Boero, F.; Taviani, M.; Tursi, A.

    2010-03-01

    The biodiversity of the Santa Maria di Leuca (SML) coral bank is summarized and its description is updated using data collected by means of underwater video systems, benthic samplers and fishing gears. A total of 222 living species have been recorded within the coral bank area in the depth range 280-1121 m. The most abundant benthic taxa recorded are Porifera (36 species) followed by Mollusca (35) and Cnidaria (31). The scleractinian corals Madrepora oculata and Lophelia pertusa are the main colonial species in the structure of the SML bank. Annelida, Crustacea and Bryozoa have been found with 24, 23 and 19 species, respectively. A total of 40 species of demersal fish have been recorded. Other faunal taxa were found with small numbers of species. One hundred and thirty-five species are new for the SML bank, 31 of which represent new records for the north-western Ionian Sea (2 Porifera, 17 Cnidaria, 1 Mollusca, 3 Annelida, 2 Crustacea, 4 Bryozoa and 4 Echinodermata). The finding of the annelid Harmothoë vesiculosa represents the first record for the Mediterranean Sea. The SML coral bank represents a biodiversity "hot-spot" on the bathyal bottoms of the Mediterranean Sea.

  15. Imaging intracellular pH in a reef coral and symbiotic anemone

    PubMed Central

    Venn, A. A.; Tambutté, E.; Lotto, S.; Zoccola, D.; Allemand, D.; Tambutté, S.

    2009-01-01

    The challenges corals and symbiotic cnidarians face from global environmental change brings new urgency to understanding fundamental elements of their physiology. Intracellular pH (pHi) influences almost all aspects of cellular physiology but has never been described in anthozoans or symbiotic cnidarians, despite its pivotal role in carbon concentration for photosynthesis and calcification. Using confocal microscopy and the pH sensitive probe carboxy SNARF-1, we mapped pHi in short-term light and dark-incubated cells of the reef coral Stylophora pistillata and the symbiotic anemone Anemonia viridis. In all cells isolated from both species, pHi was markedly lower than the surrounding seawater pH of 8.1. In cells that contained symbiotic algae, mean values of pHi were significantly higher in light treated cells than dark treated cells (7.41 ± 0.22 versus 7.13 ± 0.24 for S. pistillata; and 7.29 ± 0.15 versus 7.01 ± 0.27 for A. viridis). In contrast, there was no significant difference in pHi in light and dark treated cells without algal symbionts. Close inspection of the interface between host cytoplasm and algal symbionts revealed a distinct area of lower pH adjacent to the symbionts in both light and dark treated cells, possibly associated with the symbiosome membrane complex. These findings are significant developments for the elucidation of models of inorganic carbon transport for photosynthesis and calcification and also provide a cell imaging procedure for future investigations into how pHi and other fundamental intracellular parameters in corals respond to changes in the external environment such as reductions in seawater pH. PMID:19720994

  16. Imaging intracellular pH in a reef coral and symbiotic anemone.

    PubMed

    Venn, A A; Tambutté, E; Lotto, S; Zoccola, D; Allemand, D; Tambutté, S

    2009-09-29

    The challenges corals and symbiotic cnidarians face from global environmental change brings new urgency to understanding fundamental elements of their physiology. Intracellular pH (pHi) influences almost all aspects of cellular physiology but has never been described in anthozoans or symbiotic cnidarians, despite its pivotal role in carbon concentration for photosynthesis and calcification. Using confocal microscopy and the pH sensitive probe carboxy SNARF-1, we mapped pHi in short-term light and dark-incubated cells of the reef coral Stylophora pistillata and the symbiotic anemone Anemonia viridis. In all cells isolated from both species, pHi was markedly lower than the surrounding seawater pH of 8.1. In cells that contained symbiotic algae, mean values of pHi were significantly higher in light treated cells than dark treated cells (7.41 +/- 0.22 versus 7.13 +/- 0.24 for S. pistillata; and 7.29 +/- 0.15 versus 7.01 +/- 0.27 for A. viridis). In contrast, there was no significant difference in pHi in light and dark treated cells without algal symbionts. Close inspection of the interface between host cytoplasm and algal symbionts revealed a distinct area of lower pH adjacent to the symbionts in both light and dark treated cells, possibly associated with the symbiosome membrane complex. These findings are significant developments for the elucidation of models of inorganic carbon transport for photosynthesis and calcification and also provide a cell imaging procedure for future investigations into how pHi and other fundamental intracellular parameters in corals respond to changes in the external environment such as reductions in seawater pH. PMID:19720994

  17. Characterization of novel orange fluorescent protein cloned from cnidarian tube anemone Cerianthus sp.

    PubMed

    Ip, Denis Tsz-Ming; Wong, Kam-Bo; Wan, David Chi-Cheong

    2007-01-01

    A novel orange fluorescent protein (OFP) was cloned from the tentacles of Cnidarian tube anemone Cerianthus sp. It consists of 222 amino acid residues with a calculated molecular mass of 25.1 kDa. A BLAST protein sequence homology search revealed that native OFP has 81% sequence identity to Cerianthus membranaceus green fluorescent protein (cmFP512), 38% identity to Entacmaea quadricolor red fluorescent protein (eqFP611), 37% identity to Discosoma red fluorescent protein (DsRed), 36% identity to Fungia concinna Kusabira-orange fluorescent protein (KO), and a mere 21% identity to green fluorescent protein (GFP). It is most likely that OFP also adopts the 11-strand beta-barrel structure of fluorescent proteins. Spectroscopic analysis indicated that it has a wide absorption spectrum peak at 548 nm with two shoulders at 487 and 513 nm. A bright orange fluorescence maximum at 573 nm was observed when OFP was excited at 515 nm or above. When OFP was excited well below 515 nm, a considerable amount of green emission maximum at 513 nm was also observed. It has a fluorescence quantum yield (Phi) of 0.64 at 25 degrees C. The molar absorption coefficients (epsilon) of folded OFP at 278 and 548 nm are 47,000 and 60,000 M(-1) x cm(-1), respectively. Its fluorescent brightness (epsilon Phi) at 25 degrees C is 38,400 M(-1) x cm(-1). Like other orange-red fluorescent proteins, OFP is also tetrameric. It was readily expressed as soluble protein in Escherichia coli at 37 degrees C, and no aggregate was observed in transfected HeLa cells under our experimental conditions. Fluorescent intensity of OFP is detectable over a pH range of 3 to 12. PMID:17530459

  18. Anemone-like nanostructures for non-lithographic, reproducible, large-area, and ultra-sensitive SERS substrates.

    PubMed

    Daglar, Bihter; Demirel, Gokcen Birlik; Khudiyev, Tural; Dogan, Tamer; Tobail, Osama; Altuntas, Sevde; Buyukserin, Fatih; Bayindir, Mehmet

    2014-11-01

    The melt-infiltration technique enables the fabrication of complex nanostructures for a wide range of applications in optics, electronics, biomaterials, and catalysis. Here, anemone-like nanostructures are produced for the first time under the surface/interface principles of melt-infiltration as a non-lithographic method. Functionalized anodized aluminum oxide (AAO) membranes are used as templates to provide large-area production of nanostructures, and polycarbonate (PC) films are used as active phase materials. In order to understand formation dynamics of anemone-like structures finite element method (FEM) simulations are performed and it is found that wetting behaviour of the polymer is responsible for the formation of cavities at the caps of the structures. These nanostructures are examined in the surface-enhanced-Raman-spectroscopy (SERS) experiment and they exhibit great potential in this field. Reproducible SERS signals are detected with relative standard deviations (RSDs) of 7.2-12.6% for about 10,000 individual spots. SERS measurements are demonstrated at low concentrations of Rhodamine 6G (R6G), even at the picomolar level, with an enhancement factor of ?10(11). This high enhancement factor is ascribed to the significant electric field enhancement at the cavities of nanostructures and nanogaps between them, which is supported by finite difference time-domain (FDTD) simulations. These novel nanostructured films can be further optimized to be used in chemical and plasmonic sensors and as a single molecule SERS detection platform. PMID:25220106

  19. Specific inflammatory response of Anemonia sulcata (Cnidaria) after bacterial injection causes tissue reaction and enzymatic activity alteration.

    PubMed

    Trapani, M R; Parisi, M G; Parrinello, D; Sanfratello, M A; Benenati, G; Palla, F; Cammarata, M

    2016-03-01

    The evolution of multicellular organisms was marked by adaptations to protect against pathogens. The mechanisms for discriminating the ''self'' from ''non-self" have evolved into a long history of cellular and molecular strategies, from damage repair to the co-evolution of host-pathogen interactions. We investigated the inflammatory response in Anemonia sulcata (Cnidaria: Anthozoa) following injection of substances that varied in type and dimension, and observed clear, strong and specific reactions, especially after injection of Escherichia coli and Vibrio alginolyticus. Moreover, we analyzed enzymatic activity of protease, phosphatase and esterase, showing how the injection of different bacterial strains alters the expression of these enzymes and suggesting a correlation between the appearance of the inflammatory reaction and the modification of enzymatic activities. Our study shows for the first time, a specific reaction and enzymatic responses following injection of bacteria in a cnidarian. PMID:26836977

  20. Yolk formation in a stony coral Euphyllia ancora (Cnidaria, Anthozoa): insight into the evolution of vitellogenesis in nonbilaterian animals.

    PubMed

    Shikina, Shinya; Chen, Chieh-Jhen; Chung, Yi-Jou; Shao, Zi-Fan; Liou, Jhe-Yu; Tseng, Hua-Pin; Lee, Yan-Horn; Chang, Ching-Fong

    2013-09-01

    Vitellogenin (Vg) is a major yolk protein precursor in numerous oviparous animals. Numerous studies in bilateral oviparous animals have shown that Vg sequences are conserved across taxa and that Vgs are synthesized by somatic-cell lineages, transported to and accumulated in oocytes, and eventually used for supporting embryogenesis. In nonbilateral animals (Polifera, Cnidaria, and Ctenophora), which are regarded as evolutionarily primitive, although Vg cDNA has been identified in 2 coral species from Cnidaria, relatively little is known about the characteristics of yolk formation in their bodies. To address this issue, we identified and characterized 2 cDNA encoding yolk proteins, Vg and egg protein (Ep), in the stony coral Euphyllia ancora. RT-PCR analysis revealed that expression levels of both Vg and Ep increased in the female colonies as coral approached the spawning season. In addition, high levels of both Vg and Ep transcripts were detected in the putative ovarian tissue, as determined by tissue distribution analysis. Further analyses using mRNA in situ hybridization and immunohistochemistry determined that, within the putative ovarian tissue, these yolk proteins are synthesized in the mesenterial somatic cells but not in oocytes themselves. Furthermore, Vg proteins that accumulated in eggs were most likely consumed during the coral embryonic development, as assessed by immunoblotting. The characteristics of Vg that we identified in corals were somewhat similar to those of Vg in bilaterian oviparous animals, raising the hypothesis that such characteristics were likely present in the oogenesis of some common ancestor prior to divergence of the cnidarian and bilaterian lineages. PMID:23766130

  1. Anemone-like nanostructures for non-lithographic, reproducible, large-area, and ultra-sensitive SERS substrates

    NASA Astrophysics Data System (ADS)

    Daglar, Bihter; Demirel, Gokcen Birlik; Khudiyev, Tural; Dogan, Tamer; Tobail, Osama; Altuntas, Sevde; Buyukserin, Fatih; Bayindir, Mehmet

    2014-10-01

    The melt-infiltration technique enables the fabrication of complex nanostructures for a wide range of applications in optics, electronics, biomaterials, and catalysis. Here, anemone-like nanostructures are produced for the first time under the surface/interface principles of melt-infiltration as a non-lithographic method. Functionalized anodized aluminum oxide (AAO) membranes are used as templates to provide large-area production of nanostructures, and polycarbonate (PC) films are used as active phase materials. In order to understand formation dynamics of anemone-like structures finite element method (FEM) simulations are performed and it is found that wetting behaviour of the polymer is responsible for the formation of cavities at the caps of the structures. These nanostructures are examined in the surface-enhanced-Raman-spectroscopy (SERS) experiment and they exhibit great potential in this field. Reproducible SERS signals are detected with relative standard deviations (RSDs) of 7.2-12.6% for about 10 000 individual spots. SERS measurements are demonstrated at low concentrations of Rhodamine 6G (R6G), even at the picomolar level, with an enhancement factor of ~1011. This high enhancement factor is ascribed to the significant electric field enhancement at the cavities of nanostructures and nanogaps between them, which is supported by finite difference time-domain (FDTD) simulations. These novel nanostructured films can be further optimized to be used in chemical and plasmonic sensors and as a single molecule SERS detection platform.The melt-infiltration technique enables the fabrication of complex nanostructures for a wide range of applications in optics, electronics, biomaterials, and catalysis. Here, anemone-like nanostructures are produced for the first time under the surface/interface principles of melt-infiltration as a non-lithographic method. Functionalized anodized aluminum oxide (AAO) membranes are used as templates to provide large-area production of nanostructures, and polycarbonate (PC) films are used as active phase materials. In order to understand formation dynamics of anemone-like structures finite element method (FEM) simulations are performed and it is found that wetting behaviour of the polymer is responsible for the formation of cavities at the caps of the structures. These nanostructures are examined in the surface-enhanced-Raman-spectroscopy (SERS) experiment and they exhibit great potential in this field. Reproducible SERS signals are detected with relative standard deviations (RSDs) of 7.2-12.6% for about 10 000 individual spots. SERS measurements are demonstrated at low concentrations of Rhodamine 6G (R6G), even at the picomolar level, with an enhancement factor of ~1011. This high enhancement factor is ascribed to the significant electric field enhancement at the cavities of nanostructures and nanogaps between them, which is supported by finite difference time-domain (FDTD) simulations. These novel nanostructured films can be further optimized to be used in chemical and plasmonic sensors and as a single molecule SERS detection platform. Electronic supplementary information (ESI) available: SEM images of the AAO membrane and bare polymer film, FEM simulations of anemone-like polymeric nanopillars depending on the time and pressure, and detailed calculation of the enhancement factor both including experimental and theoretical approaches. See DOI: 10.1039/c4nr03909b

  2. Long-term fluctuations of Pelagia noctiluca (Cnidaria, Scyphomedusa) in the western Mediterranean Sea. Prediction by climatic variables

    NASA Astrophysics Data System (ADS)

    Goy, Jacquelinn; Morand, Pierre; Etienne, Michéle

    1989-02-01

    The archives of the Station Zoologique at Villefranche-sur-Mer contain records of "years with Pelagia noctiluca" and 'years without Pelagia". These records, plus additional data, indicate that over the past 200 years (1785-1985) outburst of Pelagia have occured about every 12 years. Using a forecasting model, climatic variables, notably temperature, rainfall and atmospheric pressure, appear to predict "years with Pelagia".

  3. Phenotypic selection and characterization of randomly produced non-haemolytic mutants of the toxic sea anemone protein sticholysin II.

    PubMed

    Alegre-Cebollada, Jorge; Lacadena, Valle; Oñaderra, Mercedes; Mancheño, José M; Gavilanes, José G; del Pozo, Alvaro Martínez

    2004-09-24

    A rapid screening method for haemolytic activity, using blood agar plates, has been developed to analyze randomly produced mutant variants of the pore-forming protein sticholysin II (Stn II). Those exhibiting a reduced activity were selected and the DNA corresponding to each Stn II variant sequenced. Once the mutation produced was determined, protein variants were isolated and characterized in terms of structure (circular dichroism spectra and thermal stability) and haemolytic activity. Three single mutation protein variants, at residues K19, F106 and Y111, showed a significantly decreased haemolytic activity while their thermostability was identical to that of the wild-type protein. Considering the obtained data and based on the three-dimensional structure of the protein, the role of these residues on the mechanism of haemolysis has been analyzed. PMID:15388326

  4. Leptohelia flexibilis gen. nov. et sp. nov., a remarkable deep-sea stylasterid (Cnidaria: Hydrozoa: Stylasteridae) from the southwest Pacific.

    PubMed

    Lindner, Alberto; Cairns, Stephen D; Zibrowius, Helmut

    2014-01-01

    Leptohelia flexibilis gen. nov. et sp. nov., the first stylasterid with a combined calcified and non-calcified skeleton, is described from seamounts and the slope off the islands of New Caledonia, in the southwestern Pacific. The new species is distinguished from all other species of the family Stylasteridae by having a non-calcified organic axis, internal to the basal portion of the calcified corallum. The internal axis is flexible and enclosed by a series of up to 10 calcified annuli, allowing passive lateral bending of the colony. Molecular phylogenetic analyses confirm that Leptohelia flexibilis is a stylasterid coral and reveal that the species is closely related to Leptohelia microstylus comb. nov., a southwestern Pacific stylasterid that lacks an internal axis. PMID:25543758

  5. Nanoporous TiO2 nanoparticle assemblies with mesoscale morphologies: nano-cabbage versus sea-anemone

    NASA Astrophysics Data System (ADS)

    Darbandi, Masih; Gebre, Tesfaye; Mitchell, Lucas; Erwin, William; Bardhan, Rizia; Levan, M. Douglas; Mochena, Mogus D.; Dickerson, James H.

    2014-05-01

    We report the novel synthesis of nanoporous TiO2 nanoparticle ensembles with unique mesoscale morphologies. Constituent nanoparticles evolved into multifaceted assemblies, exhibiting excellent crystallinity and enhanced photocatalytic activity compared with commercial TiO2. Such materials could be exploited for applications, like organic pollutant degradation.We report the novel synthesis of nanoporous TiO2 nanoparticle ensembles with unique mesoscale morphologies. Constituent nanoparticles evolved into multifaceted assemblies, exhibiting excellent crystallinity and enhanced photocatalytic activity compared with commercial TiO2. Such materials could be exploited for applications, like organic pollutant degradation. Electronic supplementary information (ESI) available: Synthesis and characterization procedures, TEM/XRD of samples prepared at different temperature and water content, table of nitrogen adsorption-desorption values of different samples. See DOI: 10.1039/c3nr06154j

  6. Evolutionary origins of germline segregation in Metazoa: evidence for a germ stem cell lineage in the coral Orbicella faveolata (Cnidaria, Anthozoa).

    PubMed

    Barfield, Sarah; Aglyamova, Galina V; Matz, Mikhail V

    2016-01-13

    The ability to segregate a committed germ stem cell (GSC) lineage distinct from somatic cell lineages is a characteristic of bilaterian Metazoans. However, the occurrence of GSC lineage specification in basally branching Metazoan phyla, such as Cnidaria, is uncertain. Without an independently segregated GSC lineage, germ cells and their precursors must be specified throughout adulthood from continuously dividing somatic stem cells, generating the risk of propagating somatic mutations within the individual and its gametes. To address the potential for existence of a GSC lineage in Anthozoa, the sister-group to all remaining Cnidaria, we identified moderate- to high-frequency somatic mutations and their potential for gametic transfer in the long-lived coral Orbicella faveolata (Anthozoa, Cnidaria) using a 2b-RAD sequencing approach. Our results demonstrate that somatic mutations can drift to high frequencies (up to 50%) and can also generate substantial intracolonial genetic diversity. However, these somatic mutations are not transferable to gametes, signifying the potential for an independently segregated GSC lineage in O. faveolata. In conjunction with previous research on germ cell development in other basally branching Metazoan species, our results suggest that the GSC system may be a Eumetazoan characteristic that evolved in association with the emergence of greater complexity in animal body plan organization and greater specificity of stem cell functions. PMID:26763699

  7. Cnidarian microRNAs frequently regulate targets by cleavage

    PubMed Central

    Moran, Yehu; Fredman, David; Praher, Daniela; Li, Xin Z.; Wee, Liang Meng; Rentzsch, Fabian; Zamore, Phillip D.; Technau, Ulrich; Seitz, Hervé

    2014-01-01

    In bilaterians, which comprise most of extant animals, microRNAs (miRNAs) regulate the majority of messenger RNAs (mRNAs) via base-pairing of a short sequence (the miRNA “seed”) to the target, subsequently promoting translational inhibition and transcript instability. In plants, many miRNAs guide endonucleolytic cleavage of highly complementary targets. Because little is known about miRNA function in nonbilaterian animals, we investigated the repertoire and biological activity of miRNAs in the sea anemone Nematostella vectensis, a representative of Cnidaria, the sister phylum of Bilateria. Our work uncovers scores of novel miRNAs in Nematostella, increasing the total miRNA gene count to 87. Yet only a handful are conserved in corals and hydras, suggesting that microRNA gene turnover in Cnidaria greatly exceeds that of other metazoan groups. We further show that Nematostella miRNAs frequently direct the cleavage of their mRNA targets via nearly perfect complementarity. This mode of action resembles that of small interfering RNAs (siRNAs) and plant miRNAs. It appears to be common in Cnidaria, as several of the miRNA target sites are conserved among distantly related anemone species, and we also detected miRNA-directed cleavage in Hydra. Unlike in bilaterians, Nematostella miRNAs are commonly coexpressed with their target transcripts. In light of these findings, we propose that post-transcriptional regulation by miRNAs functions differently in Cnidaria and Bilateria. The similar, siRNA-like mode of action of miRNAs in Cnidaria and plants suggests that this may be an ancestral state. PMID:24642861

  8. De Novo Assembly and Characterization of Four Anthozoan (Phylum Cnidaria) Transcriptomes

    PubMed Central

    Kitchen, Sheila A.; Crowder, Camerron M.; Poole, Angela Z.; Weis, Virginia M.; Meyer, Eli

    2015-01-01

    Many nonmodel species exemplify important biological questions but lack the sequence resources required to study the genes and genomic regions underlying traits of interest. Reef-building corals are famously sensitive to rising seawater temperatures, motivating ongoing research into their stress responses and long-term prospects in a changing climate. A comprehensive understanding of these processes will require extending beyond the sequenced coral genome (Acropora digitifera) to encompass diverse coral species and related anthozoans. Toward that end, we have assembled and annotated reference transcriptomes to develop catalogs of gene sequences for three scleractinian corals (Fungia scutaria, Montastraea cavernosa, Seriatopora hystrix) and a temperate anemone (Anthopleura elegantissima). High-throughput sequencing of cDNA libraries produced ?20–30 million reads per sample, and de novo assembly of these reads produced ?75,000–110,000 transcripts from each sample with size distributions (mean ?1.4 kb, N50 ?2 kb), comparable to the distribution of gene models from the coral genome (mean ?1.7 kb, N50 ?2.2 kb). Each assembly includes matches for more than half the gene models from A. digitifera (54–67%) and many reasonably complete transcripts (?5300–6700) spanning nearly the entire gene (ortholog hit ratios ?0.75). The catalogs of gene sequences developed in this study made it possible to identify hundreds to thousands of orthologs across diverse scleractinian species and related taxa. We used these sequences for phylogenetic inference, recovering known relationships and demonstrating superior performance over phylogenetic trees constructed using single mitochondrial loci. The resources developed in this study provide gene sequences and genetic markers for several anthozoan species. To enhance the utility of these resources for the research community, we developed searchable databases enabling researchers to rapidly recover sequences for genes of interest. Our analysis of de novo assembly quality highlights metrics that we expect will be useful for evaluating the relative quality of other de novo transcriptome assemblies. The identification of orthologous sequences and phylogenetic reconstruction demonstrates the feasibility of these methods for clarifying the substantial uncertainties in the existing scleractinian phylogeny. PMID:26384772

  9. Population genetics of the invasive cryptogenic anemone, Anemonia alicemartinae, along the southeastern Pacific coast

    NASA Astrophysics Data System (ADS)

    Canales-Aguirre, C. B.; Quiñones, A.; Hernández, C. E.; Neill, P. E.; Brante, A.

    2015-08-01

    One of the most important issues in biological invasions is understanding the factors and mechanisms determining the invasion success of non-native species. Theoretical and empirical works have shown that genetic diversity is a determinant of invasion success; thus, studying spatial patterns of genetic diversity, and exploring how biological and physical factors shape this population trait, are fundamental for understanding this phenomenon. Coastal marine ecosystems are one of the most susceptible habitats to invasion given the complex network of maritime transport. In this work we study the cryptogenic anemone, Anemonia alicemartinae, which has rapidly increased its geographical range southward during the last 50 years (approx. 2000 km) along the southeastern Pacific coast. Based on COI mtDNA sequences we evaluated three main hypotheses: a) the genetic diversity of A. alicemartinae decreases according to the direction of invasion (from north to south); b) there is biogeographic-phylogeographic concordance at the 30°S biogeographic break; and c) the demographic history is coherent with a recent geographic expansion. A total of 161 individual samples of A. alicemartinae were collected along the southeastern Pacific coast range of distribution, covering more than 2000 km, including samples along the 30°S biogeographical break. Results showed low genetic diversity (Hd = 0.253; π = 0.08) and a lack of geographic population genetic structure (FST = - 0.009, p-value = 0.656). The highest genetic diversity was observed in Peru (Chero and Mesas) and at localities close to the main Chilean seaports. We did not observe concordance between biogeographic and phylogeographic patterns or isolation by distance. Demographic indices (D = - 2.604, p < 0.001; Fu's = - 26.619, p < 0.001), as well as a star-like configuration of the haplotype network support recent population expansion of this species. Our results, together with historical field observations, support the idea that the current distribution of A. alicemartinae may be explained by an increase in population size from one small ancestral population probably from the south of Peru, with subsequent human-mediated southward transport, probably associated with regional-scale maritime activities.

  10. De Novo Assembly and Characterization of Four Anthozoan (Phylum Cnidaria) Transcriptomes.

    PubMed

    Kitchen, Sheila A; Crowder, Camerron M; Poole, Angela Z; Weis, Virginia M; Meyer, Eli

    2015-11-01

    Many nonmodel species exemplify important biological questions but lack the sequence resources required to study the genes and genomic regions underlying traits of interest. Reef-building corals are famously sensitive to rising seawater temperatures, motivating ongoing research into their stress responses and long-term prospects in a changing climate. A comprehensive understanding of these processes will require extending beyond the sequenced coral genome (Acropora digitifera) to encompass diverse coral species and related anthozoans. Toward that end, we have assembled and annotated reference transcriptomes to develop catalogs of gene sequences for three scleractinian corals (Fungia scutaria, Montastraea cavernosa, Seriatopora hystrix) and a temperate anemone (Anthopleura elegantissima). High-throughput sequencing of cDNA libraries produced ~20-30 million reads per sample, and de novo assembly of these reads produced ~75,000-110,000 transcripts from each sample with size distributions (mean ~1.4 kb, N50 ~2 kb), comparable to the distribution of gene models from the coral genome (mean ~1.7 kb, N50 ~2.2 kb). Each assembly includes matches for more than half the gene models from A. digitifera (54-67%) and many reasonably complete transcripts (~5300-6700) spanning nearly the entire gene (ortholog hit ratios ≥0.75). The catalogs of gene sequences developed in this study made it possible to identify hundreds to thousands of orthologs across diverse scleractinian species and related taxa. We used these sequences for phylogenetic inference, recovering known relationships and demonstrating superior performance over phylogenetic trees constructed using single mitochondrial loci. The resources developed in this study provide gene sequences and genetic markers for several anthozoan species. To enhance the utility of these resources for the research community, we developed searchable databases enabling researchers to rapidly recover sequences for genes of interest. Our analysis of de novo assembly quality highlights metrics that we expect will be useful for evaluating the relative quality of other de novo transcriptome assemblies. The identification of orthologous sequences and phylogenetic reconstruction demonstrates the feasibility of these methods for clarifying the substantial uncertainties in the existing scleractinian phylogeny. PMID:26384772

  11. A genomewide survey of bHLH transcription factors in the coral Acropora digitifera identifies three novel orthologous families, pearl, amber, and peridot.

    PubMed

    Gyoja, Fuki; Kawashima, Takeshi; Satoh, Nori

    2012-04-01

    Decoding the genome of the coral, Acropora digitifera, enabled us to characterize a nearly full set of 70 basic helix-loop-helix (bHLH) transcription factors in this organism. This number is comparable to 68 bHLH genes in the sea anemone, Nematostella vectensis, and larger than those in most other invertebrate metazoans. The 70 bHLH genes were assigned to 29 orthologous families previously reported. In addition, we identified three novel HLH orthologous families, which we designated pearl, amber, and peridot, increasing the number of orthologous families to 32. Pearl and amber orthologues were found in genomes and expressed sequenced tags (ESTs) of Mollusca and Annelida in addition to Cnidaria. Peridot orthologues were found in genomes and ESTs of Cephalochordata and Hemichordata in addition to Cnidaria. These three genes were likely lost in the clades of Drosophila melanogaster, Caenorhabditis elegans, and Homo sapiens during animal evolution. PMID:22419240

  12. Nematocyst discharge in Pelagia noctiluca (Cnidaria, Scyphozoa) oral arms can be affected by lidocaine, ethanol, ammonia and acetic acid.

    PubMed

    Morabito, Rossana; Marino, Angela; Dossena, Silvia; La Spada, Giuseppa

    2014-06-01

    Nematocyst discharge and concomitant delivery of toxins is triggered to perform both defence and predation strategies in Cnidarians, and may lead to serious local and systemic reactions in humans. Pelagia noctiluca (Cnidaria, Scyphozoa) is a jellyfish particularly abundant in the Strait of Messina (Italy). After accidental contact with this jellyfish, not discharged nematocysts or even fragments of tentacles or oral arms may tightly adhere to the human skin and, following discharge, severely increase pain and the other adverse consequences of the sting. The aim of the present study is to verify if the local anesthetic lidocaine and other compounds, like alcohols, acetic acid and ammonia, known to provide pain relief after jellyfish stings, may also affect in situ discharge of nematocysts. Discharge was induced by a combined physico-chemical stimulation of oral arms by chemosensitizers (such as N-acetylated sugars, aminoacids, proteins and nucleotides), in the presence or absence of 1% lidocaine, 70% ethanol, 5% acetic acid or 20% ammonia, followed by mechanical stimulation by a non-vibrating test probe. The above mentioned compounds failed to induce discharge per se, and dramatically impaired the chemosensitizer-induced discharge response. We therefore suggest that prompt local treatment of the stung epidermis with lidocaine, acetic acid, ethanol and ammonia may provide substantial pain relief and help in reducing possible harmful local and systemic adverse reaction following accidental contact with P. noctiluca specimens. PMID:24637105

  13. A Diploblastic Radiate Animal at the Dawn of Cambrian Diversification with a Simple Body Plan: Distinct from Cnidaria?

    PubMed Central

    Yasui, Kinya; Reimer, James D.; Liu, Yunhuan; Yao, Xiaoyong; Kubo, Daisuke; Shu, Degan; Li, Yong

    2013-01-01

    Background Microfossils of the genus Punctatus include developmental stages such as blastula, gastrula, and hatchlings, and represent the most complete developmental sequence of animals available from the earliest Cambrian. Despite the extremely well-preserved specimens, the evolutionary position of Punctatus has relied only on their conical remains and they have been tentatively assigned to cnidarians. We present a new interpretation of the Punctatus body plan based on the developmental reconstruction aided by recent advances in developmental biology. Results Punctatus developed from a rather large egg, gastrulated in a mode of invagination from a coeloblastura, and then formed a mouth directly from the blastopore. Spiny benthic hatchlings were distinguishable from swimming or crawling ciliate larvae found in cnidarians and sponges. A mouth appeared at the perihatching embryonic stage and was renewed periodically during growth, and old mouths transformed into the body wall, thus elongating the body. Growing animals retained a small blind gut in a large body cavity without partitioning by septa and did not form tentacles, pedal discs or holdfasts externally. A growth center at the oral pole was sufficient for body patterning throughout life, and the body patterning did not show any bias from radial symmetry. Conclusions Contrary to proposed cnidarian affinity, the Punctatus body plan has basic differences from that of cnidarians, especially concerning a spacious body cavity separating ectoderm from endoderm. The lack of many basic cnidarian characters in the body patterning of Punctatus leads us to consider its own taxonomic group, potentially outside of Cnidaria. PMID:23840375

  14. A new species of hydra (Cnidaria: Hydrozoa: Hydridae) and molecular phylogenetic analysis of six congeners from China.

    PubMed

    Wang, An-Tai; Deng, Li; Liu, Hong-Tao

    2012-12-01

    A new species of genus Hydra (Cnidaria: Hydrozoa: Hydridae), Hydra shenzhensis sp. nov. from Guangdong Province, China, is described and illustrated. Most polyps have five tentacles. Column length reaches 11 mm when relaxed. Buds do not acquire tentacles synchronously. Stenotele is broad and pyriform in shape, 1.2 times as long as its width. Holotrichous isorhiza is asymmetrical and slender (more than 2.7 times as long as its width), with transverse and slanting coils. Atrichous isorhiza is long, resembling a melon-seed in shape. Desmoneme is asymmetrically pyriform in shape. The new species, belonging to the vulgaris group, is dioecious; sexual reproduction was found to occur mostly during November and December under conditions of dense culture or food shortage. Two to thirteen testes, cone-like shape with papilla, formed beneath the tentacles. One to three ovaries, with an egg cup, milky white in color, formed on body column. Ninety percent of individuals developed only one ovum. On a mother polyp, a fertilized ovum developed an embryonic theca covering its surface. The embryotheca is brown, with a spine-like structure, covering a layer of transparent, membrane-like material. For phylogenetic analysis, the mitochondrial cytochrome oxidase subunit I gene (COI) of six hydra species collected from China was amplified by polymerase chain reaction (PCR) and sequenced. Morphological characters in combination with molecular evidence support the hydra described here as a new species. PMID:23215978

  15. Mitochondrial genome of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa): A linear DNA molecule encoding a putative DNA-dependent DNA polymerase.

    PubMed

    Shao, Zhiyong; Graf, Shannon; Chaga, Oleg Y; Lavrov, Dennis V

    2006-10-15

    The 16,937-nuceotide sequence of the linear mitochondrial DNA (mt-DNA) molecule of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa) - the first mtDNA sequence from the class Scypozoa and the first sequence of a linear mtDNA from Metazoa - has been determined. This sequence contains genes for 13 energy pathway proteins, small and large subunit rRNAs, and methionine and tryptophan tRNAs. In addition, two open reading frames of 324 and 969 base pairs in length have been found. The deduced amino-acid sequence of one of them, ORF969, displays extensive sequence similarity with the polymerase [but not the exonuclease] domain of family B DNA polymerases, and this ORF has been tentatively identified as dnab. This is the first report of dnab in animal mtDNA. The genes in A. aurita mtDNA are arranged in two clusters with opposite transcriptional polarities; transcription proceeding toward the ends of the molecule. The determined sequences at the ends of the molecule are nearly identical but inverted and lack any obvious potential secondary structures or telomere-like repeat elements. The acquisition of mitochondrial genomic data for the second class of Cnidaria allows us to reconstruct characteristic features of mitochondrial evolution in this animal phylum. PMID:16945488

  16. Differences in the protein profiles of cultured and endosymbiotic symbiodinium sp. (pyrrophyta) from the anemone aiptasia pallida (anthozoa)

    SciTech Connect

    Stochaj, W.R.; Grossman, A.R.

    1997-02-01

    One- and two-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunological analyses were used to visualize differences in polypeptides synthesized by Symbiodinium sp. from the anemone Aiptasia pallida when grown in the cultured and endosymbiotic states (freshly isolated zooxanthellae). Surprisingly, a comparison of proteins in cultured and endosymbiotic Symbiodinium sp. revealed only four major polypeptides with similar isoelectric and molecular mass characteristics. Using monospecific antibodies, we demonstrated differences in specific proteins synthesized by the dinoflagellate in the two different growth states. The dimeric, 14 kDa form of the peripheral membrane peridinin-chlorophyll a binding protein predominates under endosymbiotic conditions, whereas the monomeric, 35 kDa form predominates under the culture conditions used in this study. Antibodies to form II ribulose-1,5-bisphosphate carboxylase revealed 62 and 60 kDa forms of this protein in the alga grown as an endosymbiont and in culture, respectively. Differences in the integral membrane peridinin-chlorophyll a-c-binding proteins were also observed. These results demonstrate that there are major changes in the populations of proteins synthesized by Symbiodinium sp. in response to the conditions in hospite. Such changes may reflect a developmental switch that tailors the physiology of the alga to the conditions encountered in the endosymbiotic state. 77 refs., 6 figs.

  17. A new genus and species of pennatulacean octocoral from equatorial West Africa (Cnidaria, Anthozoa, Virgulariidae)

    PubMed Central

    Williams, Gary C.

    2015-01-01

    Abstract A new genus and species of sea pen or virgulariid pennatulacean from the Gulf of Guinea in the tropical eastern Atlantic is described, and a key to the genera of the Virgulariidae is included. The new genus and species described here adds to the previously described five other genera of the family. It is distinguished by unique sclerite and polyp leaf characters from the superficially-similar genus Virgularia, which lacks conspicuous sclerites in the polyp leaves and coenenchyme (other than minute oval bodies that are generally <0.01 mm in length). PMID:26798304

  18. Differential Gene Expression in the Siphonophore Nanomia bijuga (Cnidaria) Assessed with Multiple Next-Generation Sequencing Workflows

    PubMed Central

    Siebert, Stefan; Robinson, Mark D.; Tintori, Sophia C.; Goetz, Freya; Helm, Rebecca R.; Smith, Stephen A.; Shaner, Nathan; Haddock, Steven H. D.; Dunn, Casey W.

    2011-01-01

    We investigated differential gene expression between functionally specialized feeding polyps and swimming medusae in the siphonophore Nanomia bijuga (Cnidaria) with a hybrid long-read/short-read sequencing strategy. We assembled a set of partial gene reference sequences from long-read data (Roche 454), and generated short-read sequences from replicated tissue samples that were mapped to the references to quantify expression. We collected and compared expression data with three short-read expression workflows that differ in sample preparation, sequencing technology, and mapping tools. These workflows were Illumina mRNA-Seq, which generates sequence reads from random locations along each transcript, and two tag-based approaches, SOLiD SAGE and Helicos DGE, which generate reads from particular tag sites. Differences in expression results across workflows were mostly due to the differential impact of missing data in the partial reference sequences. When all 454-derived gene reference sequences were considered, Illumina mRNA-Seq detected more than twice as many differentially expressed (DE) reference sequences as the tag-based workflows. This discrepancy was largely due to missing tag sites in the partial reference that led to false negatives in the tag-based workflows. When only the subset of reference sequences that unambiguously have tag sites was considered, we found broad congruence across workflows, and they all identified a similar set of DE sequences. Our results are promising in several regards for gene expression studies in non-model organisms. First, we demonstrate that a hybrid long-read/short-read sequencing strategy is an effective way to collect gene expression data when an annotated genome sequence is not available. Second, our replicated sampling indicates that expression profiles are highly consistent across field-collected animals in this case. Third, the impacts of partial reference sequences on the ability to detect DE can be mitigated through workflow choice and deeper reference sequencing. PMID:21829563

  19. Differential gene expression in the siphonophore Nanomia bijuga (Cnidaria) assessed with multiple next-generation sequencing workflows.

    PubMed

    Siebert, Stefan; Robinson, Mark D; Tintori, Sophia C; Goetz, Freya; Helm, Rebecca R; Smith, Stephen A; Shaner, Nathan; Haddock, Steven H D; Dunn, Casey W

    2011-01-01

    We investigated differential gene expression between functionally specialized feeding polyps and swimming medusae in the siphonophore Nanomia bijuga (Cnidaria) with a hybrid long-read/short-read sequencing strategy. We assembled a set of partial gene reference sequences from long-read data (Roche 454), and generated short-read sequences from replicated tissue samples that were mapped to the references to quantify expression. We collected and compared expression data with three short-read expression workflows that differ in sample preparation, sequencing technology, and mapping tools. These workflows were Illumina mRNA-Seq, which generates sequence reads from random locations along each transcript, and two tag-based approaches, SOLiD SAGE and Helicos DGE, which generate reads from particular tag sites. Differences in expression results across workflows were mostly due to the differential impact of missing data in the partial reference sequences. When all 454-derived gene reference sequences were considered, Illumina mRNA-Seq detected more than twice as many differentially expressed (DE) reference sequences as the tag-based workflows. This discrepancy was largely due to missing tag sites in the partial reference that led to false negatives in the tag-based workflows. When only the subset of reference sequences that unambiguously have tag sites was considered, we found broad congruence across workflows, and they all identified a similar set of DE sequences. Our results are promising in several regards for gene expression studies in non-model organisms. First, we demonstrate that a hybrid long-read/short-read sequencing strategy is an effective way to collect gene expression data when an annotated genome sequence is not available. Second, our replicated sampling indicates that expression profiles are highly consistent across field-collected animals in this case. Third, the impacts of partial reference sequences on the ability to detect DE can be mitigated through workflow choice and deeper reference sequencing. PMID:21829563

  20. Comparison of morphological and genetic analyses reveals cryptic divergence and morphological plasticity in Stylophora (Cnidaria, Scleractinia)

    NASA Astrophysics Data System (ADS)

    Stefani, Fabrizio; Benzoni, F.; Yang, S.-Y.; Pichon, M.; Galli, P.; Chen, C. A.

    2011-12-01

    A combined morphological and genetic study of the coral genus Stylophora investigated species boundaries in the Gulf of Aden, Yemen. Two mitochondrial regions, including the hypervariable IGS9 spacer and the control region, and a fragment of rDNA were used for phylogenetic analysis. Results were compared by multivariate analysis on the basis of branch morphology and corallite morphometry. Two species were clearly discriminated by both approaches. The first species was characterised by small corallites and a low morphological variability and was ascribed to a new geographical record of Stylophora madagascarensis on the basis of its phylogenetic distinction and its morphological similarity to the type material. The second species was characterised by larger corallite size and greater morphological variability and was ascribed to Stylophora pistillata. The analysis was extended to the intrageneric level for other S. pistillata populations from the Red Sea and the Pacific Ocean. Strong internal divergence was evident in the genus Sty lophora. S. pistillata populations were split into two highly divergent Red Sea/Gulf of Aden and western Pacific lineages with significant morphological overlap, which suggests they represent two distinct cryptic species. The combined use of morphological and molecular approaches, so far proved to be a powerful tool for the re-delineation of species boundaries in corals, provided novel evidence of cryptic divergence in this group of marine metazoans.

  1. Metal bioaccumulation pattern by Cotylorhiza tuberculata (Cnidaria, Scyphozoa) in the Mar Menor coastal lagoon (SE Spain).

    PubMed

    Muñoz-Vera, Ana; García, Gregorio; García-Sánchez, Antonio

    2015-12-01

    Coastal lagoons are ecosystems highly vulnerable to human impacts because of their situation between terrestrial and marine environment. Mar Menor coastal lagoon is one of the largest lagoons of the Mediterranean Sea, placed in SE Spain and subjected to major human impacts, in particular the mining of metal sulphides. As a consequence, metal concentration in water column and sediments of this ecosystem is usually higher than in other areas. For monitoring ecosystem health, the present study has assessed the ability of Cotylorhiza tuberculata for bioaccumulating metals from sea water. Up to 65 individuals were sampled at 8 different sampling stations during the summer of 2012. Although the concentration values for different elements considered were moderate (Pb: 0.04-29.50 ppm, Zn: 2.27-93.44 ppm, Cd: 0-0.67 ppm, As: 0.56-130.31 ppm) by dry weight of the jellyfish tissues (bell and oral arms combined), bioconcentration levels in relation to seawater metal concentration were extremely high. In any case, the use or disposal of these organisms should consider their metal content because of their potential environmental and health implications. PMID:26250818

  2. Invasive Ponto-Caspian hydrozoan Cordylophora caspia (hydrozoa: Cnidaria) in southern Baltic coastal lakes

    NASA Astrophysics Data System (ADS)

    Obolewski, Krystian; Jarosiewicz, Anna; Ożgo, Małgorzata

    2015-12-01

    Cordylophora caspia Pall. is a highly invasive Ponto-Caspian colonial hydroid with a worldwide distribution. It is a biofouling organism colonizing industrial water installations and causing serious economic problems. Here, we give the first report of its occurrence in southern Baltic coastal lakes, and analyze its distribution in relation to environmental factors and likely colonization routes. Samples were collected from the stalks of Phragmites australis at the total of 102 sites in 15 lakes and lagoons. The species was most numerous in lagoons, i.e. ß-oligohaline water bodies with a surface hydrological connection with the sea, where it reached mean densities of 1200-4800 hydranths m-2. In regression tree analysis, chloride concentration, followed by pH, were the strongest explanatory variables for its occurrence, with highest densities observed at chloride concentration above 1.18 g Cl L-1 and pH 8.05-9.26. At pH 5.77-8.04 higher densities were observed at temperatures above 20.3 °C. Generally, within the range of parameters observed in our study, high densities of C. caspia were associated with high chloride concentration, pH, temperature and electrical conductivity values. The species was also present in freshwater lakes; these colonies may have the highest capacity for future invasions of such habitats. Within lakes, high densities were observed at canals connecting these water bodies with the sea, and at sites close to the inflow of rivers. This distribution pattern can facilitate its further spread into inland waters.

  3. Small-scale distribution of deep-sea demersal nekton and other megafauna in the Charlie-Gibbs Fracture Zone of the Mid-Atlantic Ridge

    NASA Astrophysics Data System (ADS)

    Felley, J. D.; Vecchione, M.; Wilson, R. R., Jr.

    2008-01-01

    Videotapes from manned submersibles diving in the area of the Charlie-Gibbs Fracture Zone of the Mid-Atlantic Ridge were used to investigate the distribution of fishes, large crustaceans, epifaunal and sessile organisms, and environmental features along a series of transects. Submersibles MIR 1 and MIR 2 conducted paired dives in an area of mixed sediment and rock (beginning depth ca. 3000 m) and on a large pocket of abyssal-like sediments (depth ca. 4000 m). In the shallower area, the submersibles passed over extremely heterogeneous terrain with a diversity of nekton, epifaunal forms and sessile forms. In the first pair of dives, MIR 1 rose along the Mid-Atlantic Ridge from 3000 to 1700 m, while MIR 2 remained near the 3000 m isobath. Nekton seen in these relatively shallow dives included large and small macrourids (genus Coryphaenoides), shrimp (infraorder Penaeidea), Halosauropsis macrochir, Aldrovandia sp., Antimora rostrata, and alepocephalids. The last two were more characteristic of the upper areas of the slope reached by MIR 1, as it rose along the Mid-Atlantic Ridge to depths less than 3000 m. Distributions of some forms seemed associated with depth and/or the presence of hard substrate. Sessile organisms such as sponges and large cnidaria were more likely to be found in rocky areas. The second pair of dives occurred in an abyssal area and the submersibles passed over sediment-covered plains, with little relief and many fewer countable organisms and features. The most evident of these were holes, mounds, small cerianthid anemones, small macrourids and the holothurian Benthodytes sp. A few large macrourids and shrimp also were seen in these deeper dives, as well as squat lobsters ( Munidopsis sp.). Sponges and larger cnidaria were mostly associated with a few small areas of rocky substrate. Holes and mounds showed distributions suggesting large-scale patterning. Over all dives, most sessile and epifaunal forms showed clumped distributions. However, large holothurians and large nekton often had distributions not significantly different from random.

  4. The Melithaeidae (Cnidaria: Octocorallia) of the Ryukyu Archipelago: molecular and morphological examinations.

    PubMed

    Aguilar-Hurtado, Catalina; Nonaka, Masanori; Reimer, James D

    2012-07-01

    The family Melithaeidae (Octocorallia: Alcyonacea) is distributed in the West Pacific, Indian Ocean and the Red Sea. They are most abundant in warmer waters but can also be found in temperate waters. At present six genera are assigned to this family (Melithaea, Mopsella, Clathraria, Acabaria, Wrightella and Asperaxis), however overlapping characteristics make this group's taxonomic identification difficult and their relationships unclear. There are only a few reports from the Ryukyu Archipelago in southern Japan of melithaeids and most other octocorals, despite the islands being an area of high octocoral diversity. To help resolve the taxonomic confusion in this family, samples from various Ryukyu Archipelago locations were collected and DNA sequences of nuclear 28S ribosomal DNA and mitochondrial cytochrome oxidase I (COI) were obtained. Additionally, SEM micrographs of the sclerites of specimens were taken to further confirm the molecular results. Three strongly supported clades were recovered from the COI and 28S rDNA analyses, corresponding to Melithaea, Acabaria, and Mopsella, and in most cases clades were clearly related with the sclerite shape reported for each genus. These results show clearly that molecular differences are present between the three genera, and also demonstrates the strong need of other molecular markers for resolving intra-generic phylogenies. Our results provide baseline data for future studies of this octocoral family, not only on taxonomy, but also with regards to their distribution in the Ryukyu Islands. PMID:22465401

  5. SYSTEMATIC MOTION OF FINE-SCALE JETS AND SUCCESSIVE RECONNECTION IN SOLAR CHROMOSPHERIC ANEMONE JET OBSERVED WITH THE SOLAR OPTICAL TELESCOPE/HINODE

    SciTech Connect

    Singh, K. A. P.; Nishida, K.; Shibata, K.; Isobe, H.

    2012-11-20

    The Solar Optical Telescope (SOT) on board Hinode allows observations with high spatiotemporal resolution and stable image quality. A {lambda}-shaped chromospheric anemone jet was observed in high resolution with SOT/Hinode. We found that several fine-scale jets were launched from one end of the footpoint to the other. These fine-scale jets ({approx}1.5-2.5 Mm) gradually move from one end of the footpoint to the other and finally merge into a single jet. This process occurs recurrently, and as time progresses the jet activity becomes more and more violent. The time evolution of the region below the jet in Ca II H filtergram images taken with SOT shows that various parts (or knots) appear at different positions. These bright knots gradually merge into each other during the maximum phase. The systematic motion of the fine-scale jets is observed when different knots merge into each other. Such morphology would arise due to the emergence of a three-dimensional twisted flux rope in which the axial component (or the guide field) appears in the later stages of the flux rope emergence. The partial appearance of the knots could be due to the azimuthal magnetic field that appears during the early stage of the flux rope emergence. If the guide field is strong and reconnection occurs between the emerging flux rope and an ambient magnetic field, this could explain the typical feature of systematic motion in chromospheric anemone jets.

  6. Cnidarian phylogenetic relationships as revealed by mitogenomics

    PubMed Central

    2013-01-01

    Background Cnidaria (corals, sea anemones, hydroids, jellyfish) is a phylum of relatively simple aquatic animals characterized by the presence of the cnidocyst: a cell containing a giant capsular organelle with an eversible tubule (cnida). Species within Cnidaria have life cycles that involve one or both of the two distinct body forms, a typically benthic polyp, which may or may not be colonial, and a typically pelagic mostly solitary medusa. The currently accepted taxonomic scheme subdivides Cnidaria into two main assemblages: Anthozoa (Hexacorallia + Octocorallia) – cnidarians with a reproductive polyp and the absence of a medusa stage – and Medusozoa (Cubozoa, Hydrozoa, Scyphozoa, Staurozoa) – cnidarians that usually possess a reproductive medusa stage. Hypothesized relationships among these taxa greatly impact interpretations of cnidarian character evolution. Results We expanded the sampling of cnidarian mitochondrial genomes, particularly from Medusozoa, to reevaluate phylogenetic relationships within Cnidaria. Our phylogenetic analyses based on a mitochogenomic dataset support many prior hypotheses, including monophyly of Hexacorallia, Octocorallia, Medusozoa, Cubozoa, Staurozoa, Hydrozoa, Carybdeida, Chirodropida, and Hydroidolina, but reject the monophyly of Anthozoa, indicating that the Octocorallia + Medusozoa relationship is not the result of sampling bias, as proposed earlier. Further, our analyses contradict Scyphozoa [Discomedusae + Coronatae], Acraspeda [Cubozoa + Scyphozoa], as well as the hypothesis that Staurozoa is the sister group to all the other medusozoans. Conclusions Cnidarian mitochondrial genomic data contain phylogenetic signal informative for understanding the evolutionary history of this phylum. Mitogenome-based phylogenies, which reject the monophyly of Anthozoa, provide further evidence for the polyp-first hypothesis. By rejecting the traditional Acraspeda and Scyphozoa hypotheses, these analyses suggest that the shared morphological characters in these groups are plesiomorphies, originated in the branch leading to Medusozoa. The expansion of mitogenomic data along with improvements in phylogenetic inference methods and use of additional nuclear markers will further enhance our understanding of the phylogenetic relationships and character evolution within Cnidaria. PMID:23302374

  7. The biology and ecology of black corals (Cnidaria: Anthozoa: Hexacorallia: Antipatharia).

    PubMed

    Wagner, Daniel; Luck, Daniel G; Toonen, Robert J

    2012-01-01

    Antipatharians, commonly known as black corals, are treasured by many cultures for medicinal purposes and to produce jewellery. Despite their economic and cultural importance, very little is known about the basic biology and ecology of black corals because most species inhabit deeper-water environments (>50m) which are logistically challenging to study. There has been a recent increase of studies focusing on antipatharians; however, these have not yet been comprehensively reviewed. This literature review seeks to summarize the available information on the biology and ecology of antipatharians. Although black corals occur throughout all oceans and from subtidal to abyssal depths, they are particularly common in tropical and subtropical regions at depths below 50m. Antipatharians are generally found in areas with hard substrates, low-light and strong currents. Under favourable conditions, some black coral species form dense aggregations to the point of becoming ecologically dominant. Zooplankton appears to be the major component of the diet of black corals, which feed as suspension feeders and use mucus and nematocysts to capture their prey. Previously categorized as azooxanthellate corals, recent research has revealed that many antipatharians appear capable of harbouring symbionts, but unlike other corals, dinoflagellates of the genus Symbiodinium are generally not important to the nutrition of black corals. Antipatharians reproduce through both sexual and asexual processes. In general, polyps and colonies are gonochoric, with fertilization and larval development likely occurring externally; however, to date antipatharian larvae have only been observed for a single species. Antipatharians are generally slow-growing and long-lived organisms with maximum longevities ranging from decades to millennia. Black corals are more abundant with depth, a pattern which has been hypothesized to avoid competition with obligate photosynthetic fauna. Additionally, antipatharians may compete for space by using sweeper tentacles and secondary metabolites. With the exception of a few predators such as gastropods and green sea turtles, antipatharians appear to be little impacted by predation. Like other corals, antipatharians can be habitat engineers of importance to a myriad of associated organisms including arthropods, annelids, echinoderms, mollusks, sponges and cnidarians, several of which are adapted to live exclusively on black corals. Given that most black coral species inhabit remote environments, our understanding of these organisms will depend on our ability to effectively sample and study them. Future collections, particularly in deeper waters (>50m), will be needed to determine whether antipatharian species have limited biogeographical distributions or whether this has simply been an artefact of low sampling efforts away from population centres and taxonomic uncertainties within this group. Additionally, biological and ecological studies require increased sample sizes because most information is currently derived from the examination of only a handful of specimens. PMID:22877611

  8. Total Saponin from Anemone flaccida Fr. Schmidt Prevents Bone Destruction in Experimental Rheumatoid Arthritis via Inhibiting Osteoclastogenesis.

    PubMed

    Liu, Chunfang; Yang, Yue; Sun, Danni; Wang, Chao; Wang, Hui; Jia, Shiwei; Liu, Liang; Lin, Na

    2015-12-01

    Anemone flaccida Fr. Schmidt is used in the clinical compound prescription for the treatment of rheumatoid arthritis (RA) in China and has the traditional use of draining dampness, diminishing swelling, and relieving pain. Total saponins (TS) are the characteristic components and also the main active ingredients of A. flaccida. Previous reports indicated that TS possess anti-inflammatory and immunoregulatory properties; however, the effects of TS on bone destruction of RA have not been evaluated. In this study, our data first showed the therapeutic effects of TS on severity of arthritis and arthritis progression in collagen-induced arthritis (CIA) rats. Then, by microfocal computed tomography (CT) quantification, TS significantly increased bone mineral density, bone volume fraction, and trabecular thickness and decreased trabecular separation of inflamed joints both at peri-articular and extra-articular locations. TS also diminished the level of the bone resorption marker CTX-I and simultaneously increased the bone formation marker osteocalcin in sera of CIA rats. Interestingly, TS prevented bone destruction by reducing the number of osteoclasts in inflamed joints, reducing the expression of receptor activator of nuclear factor-κF (RANK) ligand (RANKL) and RANK, increasing the expression of osteoprotegerin (OPG), at both mRNA and protein levels, and decreasing the ratio of RANKL to OPG in inflamed joints and sera of CIA rats. This was further confirmed in the co-culture system of human fibroblast-like synovial and peripheral blood mononuclear cells. In addition, TS inhibited the levels of pro-inflammatory cytokines implicated in bone resorption, such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNFα), IL-6, IL-17, and IL-23 in sera and joints. These findings offer convincing evidence that TS attenuate RA partially by preventing both focal bone destruction and systemic bone loss. This anti-erosive effect results in part from inhibiting osteoclastogenesis by regulating the RANKL/RANK/OPG signaling pathway. The suppression of systemic and local pro-osteoclastogenic cytokines by TS was also highly effective. PMID:26418168

  9. Life history and feeding biology of the deep-sea pycnogonid Nymphon hirtipes

    NASA Astrophysics Data System (ADS)

    Mercier, Annie; Baillon, Sandrine; Hamel, Jean-François

    2015-12-01

    Pycnogonids (sea spiders) are commonly collected at bathyal and abyssal depths all around the world; however, little is known about species from deep-water habitats. The present study explores the life history of Nymphon hirtipes collected in northeastern Newfoundland between 700 and 1450 m depth and monitored in mesocosms for over 2 years. The pycnogonids were found in association with octocorals, hydrozoans and sea anemones. Adult females developed mature oocytes between June and August. Paired mating followed by oviposition occurred between early July and mid-October. Up to three egg masses were brooded by each male. It took a maximum of 4 months for the propagules to hatch from the egg mass. Offspring developed from walking leg-bearing larvae to early juveniles over the next ~5 months before leaving the male's protection. Mating and oviposition coincided with the highest water temperatures of the annual cycle and dispersal of juveniles occurred in spring, when phytodetritus deposition was high and as ocean temperature rose markedly. All the females died after oviposition and the males ~9 months later, after juvenile dispersal. The sex ratio of mature individuals (~55-60 mm leg span) was 2 males for 3 females. Fecundity was estimated to be 184-288 eggs per adult female. Adults of N. hirtipes were seen feeding on hydrozoan polyps, small sea anemones (Stephanauge nexilis) and nudibranchs. Larvae and early juveniles did not feed while brooded by the male. Upon dispersal, their feeding apparatus became functional and they began feeding on hydrozoan polyps. After 13 months of growth post hatching, the juveniles reached ~21 mm leg span. Curve fitting estimated that ~7 years are required to reach the adult size of 55 mm leg span.

  10. Wnt signaling in the early sea urchin embryo.

    PubMed

    Kumburegama, Shalika; Wikramanayake, Athula H

    2008-01-01

    Wnt signaling regulates a remarkably diverse array of cellular and developmental events during animal embryogenesis and homeostasis. The crucial role that Wnt signaling plays in regulating axial patterning in early embryos has been particularly striking. Recent work has highlighted the conserved role that canonical Wnt signaling plays in patterning the animal-vegetal (A-V) axis in sea urchin and sea anemone embryos. In sea urchin embryos, the canonical Wnt signaling pathway is selectively turned on in vegetal cells as early as the 16-cell stage embryo, and signaling through this pathway is required for activation of the endomesodermal gene regulatory network. Loss of nuclear beta-catenin signaling animalizes the sea urchin embryo and blocks pattern formation along the entire A-V axis. Nuclear entry of beta-catenin into vegetal cells is regulated cell autonomously by maternal information that is present at the vegetal pole of the unfertilized egg. Analysis of Dishevelled (Dsh) regulation along the A-V axis has revealed the presence of a cytoarchitectural domain at the vegetal pole of the unfertilized sea urchin egg. This vegetal cortical domain appears to be crucial for the localized activation of Dsh at the vegetal pole, but the precise mechanisms are unknown. The elucidation of how Dsh is selectively activated at the vegetal cortical domain is likely to provide important insight into how this enigmatic protein is regulated during canonical Wnt signaling. Additionally, this information will shed light on the origins of embryonic polarity during animal evolution. This chapter examines the roles played by the canonical Wnt signaling pathway in the specification and patterning of the A-V axis in the sea urchin. These studies have led to the identification of a novel role for canonical Wnt signaling in regulating protein stability, and continued studies of Wnt signaling in this model system are likely to reveal additional roles for this pathway in regulating early patterning events in embryos. PMID:19109711

  11. Venom present in sea anemone (Heteractis magnifica) induces apoptosis in non-small-cell lung cancer A549 cells through activation of mitochondria-mediated pathway.

    PubMed

    Ramezanpour, Mahnaz; da Silva, Karen Burke; Sanderson, Barbara J S

    2014-03-01

    Lung cancer is a major cause of cancer deaths throughout the world and the complexity of apoptosis resistance in lung cancer is apparent. Venom from Heteractis magnifica caused dose-dependent decreases in survival of the human non-small-cell lung cancer cell line, as determined by the MTT and Crystal Violet assays. The H. magnifica venom induced cell cycle arrest and induced apoptosis of A549 cells, as confirmed by annexin V/propidium iodide staining. The venom-induced apoptosis in A549 cells was characterized by cleavage of caspase-3 and a reduction in the mitochondrial membrane potential. Interestingly, crude extracts from H. magnifica had less effect on the survival of non-cancer cell lines. In the non-cancer cells, the mechanism via which cell death occurred was through necrosis not apoptosis. These findings are important for future work using H. magnifica venom for pharmaceutical development to treat human lung cancer. PMID:24190482

  12. Phylogenetic analysis of higher-level relationships within Hydroidolina (Cnidaria: Hydrozoa) using mitochondrial genome data and insight into their mitochondrial transcription

    PubMed Central

    Bentlage, Bastian; Cartwright, Paulyn; Yanagihara, Angel A.; Lindsay, Dhugal J.; Hopcroft, Russell R.; Collins, Allen G.

    2015-01-01

    Hydrozoans display the most morphological diversity within the phylum Cnidaria. While recent molecular studies have provided some insights into their evolutionary history, sister group relationships remain mostly unresolved, particularly at mid-taxonomic levels. Specifically, within Hydroidolina, the most speciose hydrozoan subclass, the relationships and sometimes integrity of orders are highly unsettled. Here we obtained the near complete mitochondrial sequence of twenty-six hydroidolinan hydrozoan species from a range of sources (DNA and RNA-seq data, long-range PCR). Our analyses confirm previous inference of the evolution of mtDNA in Hydrozoa while introducing a novel genome organization. Using RNA-seq data, we propose a mechanism for the expression of mitochondrial mRNA in Hydroidolina that can be extrapolated to the other medusozoan taxa. Phylogenetic analyses using the full set of mitochondrial gene sequences provide some insights into the order-level relationships within Hydroidolina, including siphonophores as the first diverging clade, a well-supported clade comprised of Leptothecata-Filifera III–IV, and a second clade comprised of Aplanulata-Capitata s.s.-Filifera I–II. Finally, we describe our relatively inexpensive and accessible multiplexing strategy to sequence long-range PCR amplicons that can be adapted to most high-throughput sequencing platforms. PMID:26618080

  13. Giant deep-sea protist produces bilaterian-like traces.

    PubMed

    Matz, Mikhail V; Frank, Tamara M; Marshall, N Justin; Widder, Edith A; Johnsen, Sönke

    2008-12-01

    One of the strongest paleontological arguments in favor of the origin of bilaterally symmetrical animals (Bilateria) prior to their obvious and explosive appearance in the fossil record in the early Cambrian, 542 million years ago, is the occurrence of trace fossils shaped like elongated sinuous grooves or furrows in the Precambrian. Being restricted to the seafloor surface, these traces are relatively rare and of limited diversity, and they do not show any evidence of the use of hard appendages. They are commonly attributed to the activity of the early nonskeletonized bilaterians or, alternatively, large cnidarians such as sea anemones or sea pens. Here we describe macroscopic groove-like traces produced by a living giant protist and show that these traces bear a remarkable resemblance to the Precambrian trace fossils, including those as old as 1.8 billion years. This is the first evidence that organisms other than multicellular animals can produce such traces, and it prompts re-evaluation of the significance of Precambrian trace fossils as evidence of the early diversification of Bilateria. Our observations also render indirect support to the highly controversial interpretation of the enigmatic Ediacaran biota of the late Precambrian as giant protists. PMID:19026540

  14. Biomass of scyphozoan jellyfish, and its spatial association with 0-group fish in the Barents Sea.

    PubMed

    Eriksen, Elena; Prozorkevich, Dmitry; Trofimov, Aleksandr; Howell, Daniel

    2012-01-01

    An 0-group fish survey is conducted annually in the Barents Sea in order to estimate fish population abundance. Data on jellyfish by-catch have been recorded since 1980, although this dataset has never been analysed. In recent years, however, the ecological importance of jellyfish medusae has become widely recognized. In this paper the biomass of jellyfish (medusae) in 0-60 m depths is calculated for the period 1980-2010. During this period the climate changed from cold to warm, and changes in zooplankton and fish distribution and abundance were observed. This paper discusses the less well known ecosystem component; jellyfish medusae within the Phylum Cnidaria, and their spatial and temporal variation. The long term average was ca. 9×10⁸ kg, with some years showing biomasses in excess of 5×10⁹ kg. The biomasses were low during 1980s, increased during 1990s, and were highest in early 2000s with a subsequent decline. The bulk of the jellyfish were observed in the central parts of the Barents Sea, which is a core area for most 0-group fishes. Jellyfish were associated with haddock in the western area, with haddock and herring in the central and coastal area, and with capelin in the northern area of the Barents Sea. The jellyfish were present in the temperature interval 1°CSea jellyfish medusae; however their biomass has showed a recent moderate decline during years with record high temperatures in the Barents Sea. Jellyfish are undoubtedly an important component of the Barents Sea ecosystem, and the data presented here represent the best summary of jellyfish biomass and distribution yet published for the region. PMID:22457732

  15. Biomass of Scyphozoan Jellyfish, and Its Spatial Association with 0-Group Fish in the Barents Sea

    PubMed Central

    Eriksen, Elena; Prozorkevich, Dmitry; Trofimov, Aleksandr; Howell, Daniel

    2012-01-01

    An 0-group fish survey is conducted annually in the Barents Sea in order to estimate fish population abundance. Data on jellyfish by-catch have been recorded since 1980, although this dataset has never been analysed. In recent years, however, the ecological importance of jellyfish medusae has become widely recognized. In this paper the biomass of jellyfish (medusae) in 0–60 m depths is calculated for the period 1980–2010. During this period the climate changed from cold to warm, and changes in zooplankton and fish distribution and abundance were observed. This paper discusses the less well known ecosystem component; jellyfish medusae within the Phylum Cnidaria, and their spatial and temporal variation. The long term average was ca. 9×108 kg, with some years showing biomasses in excess of 5×109 kg. The biomasses were low during 1980s, increased during 1990s, and were highest in early 2000s with a subsequent decline. The bulk of the jellyfish were observed in the central parts of the Barents Sea, which is a core area for most 0-group fishes. Jellyfish were associated with haddock in the western area, with haddock and herring in the central and coastal area, and with capelin in the northern area of the Barents Sea. The jellyfish were present in the temperature interval 1°CSea jellyfish medusae; however their biomass has showed a recent moderate decline during years with record high temperatures in the Barents Sea. Jellyfish are undoubtedly an important component of the Barents Sea ecosystem, and the data presented here represent the best summary of jellyfish biomass and distribution yet published for the region. PMID:22457732

  16. Two Streptomyces species producing antibiotic, antitumor, and anti-inflammatory compounds are widespread among intertidal macroalgae and deep-sea coral reef invertebrates from the central Cantabrian Sea.

    PubMed

    Braña, Alfredo F; Braña, Afredo F; Fiedler, Hans-Peter; Nava, Herminio; González, Verónica; Sarmiento-Vizcaíno, Aida; Molina, Axayacatl; Acuña, José L; García, Luis A; Blanco, Gloria

    2015-04-01

    Streptomycetes are widely distributed in the marine environment, although only a few studies on their associations to algae and coral ecosystems have been reported. Using a culture-dependent approach, we have isolated antibiotic-active Streptomyces species associated to diverse intertidal marine macroalgae (Phyllum Heterokontophyta, Rhodophyta, and Chlorophyta), from the central Cantabrian Sea. Two strains, with diverse antibiotic and cytotoxic activities, were found to inhabit these coastal environments, being widespread and persistent over a 3-year observation time frame. Based on 16S rRNA sequence analysis, the strains were identified as Streptomyces cyaneofuscatus M-27 and Streptomyces carnosus M-40. Similar isolates to these two strains were also associated to corals and other invertebrates from deep-sea coral reef ecosystem (Phyllum Cnidaria, Echinodermata, Arthropoda, Sipuncula, and Anelida) living up to 4.700-m depth in the submarine Avilés Canyon, thus revealing their barotolerant feature. These two strains were also found to colonize terrestrial lichens and have been repeatedly isolated from precipitations from tropospheric clouds. Compounds with antibiotic and cytotoxic activities produced by these strains were identified by high-performance liquid chromatography (HPLC) and database comparison. Antitumor compounds with antibacterial activities and members of the anthracycline family (daunomycin, cosmomycin B, galtamycin B), antifungals (maltophilins), anti-inflamatory molecules also with antituberculosis properties (lobophorins) were identified in this work. Many other compounds produced by the studied strains still remain unidentified, suggesting that Streptomyces associated to algae and coral ecosystems might represent an underexplored promising source for pharmaceutical drug discovery. PMID:25319239

  17. Aral Sea

    Atmospheric Science Data Center

    2013-04-16

    ... the fourth-largest inland sea in the world. Since then, its water volume has dropped by about 80% due to extensive irrigation systems ... Sea has led to soil and water salination and agrochemical contamination. The retreating shoreline leaves the surface encrusted with salt ...

  18. Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection.

    PubMed

    Bince, Joanna M; Wikramanayake, Athula H

    2008-01-01

    The Wnt pathway is a highly conserved signal transduction pathway that plays many critical roles in early animal development. Recent studies have shown that this pathway plays a conserved role in the specification and patterning of the animal-vegetal (A-V) axis in sea urchins and sea anemones. These observations have suggested that the common ancestor to cnidarians and bilaterians used the Wnt signaling pathway for specifying and patterning this maternally established axis. Because the A-V axis plays a critical role in germ layer segregation, a better understanding of how the Wnt pathway is regulated along the A-V axis will provide key insight into the molecular mechanisms regulating germ layer segregation and germ layer evolution in animal embryos. Here, we provide a detailed protocol for using mRNA microinjection that can be used to analyze Wnt signaling in early sea urchin embryos. This protocol can also be adapted to introduce morpholino anti-sense oligonucleotides into sea urchin embryos. PMID:19109713

  19. Deep-sea food web analysis using cross-reacting antisera

    NASA Astrophysics Data System (ADS)

    Feller, Robert J.; Zagursky, Gregory; Day, Elizabeth A.

    1985-04-01

    The high incidence of unrecognizable prey in the stomachs of deep-sea predators prompted the application of serological methods for identification of trophic connections. Antisera to whole-organism extracts of estuarine taxa cross-reacted with antigenic protein extracts of mid-water and deep-sea taxa along phylogenetically correct lines, indicating their potential as tools for gut contents immunoassay. Stomach, intestine, and rectum contents of grenadiers ( Coryphaenoides armatus) trapped at 2500 m in the North Atlantic were analyzed visually and with 32 antisera representing taxa from 10 common deep-sea phyla. While visual analysis only revealed the presence of fluids, parasites, crustacean exoskeletons, and gastropod opercula, the immunoassay indicated the presence of antigenic proteins from holothurian, anemone, gastropod, decapod, and foraminiferan prey in the same samples. This qualitative serological identification of prey at non-specific taxonomic levels provides evidence that benthic predation may be important within deep-sea communities. The immunoassay technique, although not a panacea for elucidating food web dynamics in remote environments, may be useful when other methods fail to identify trophic pathways.

  20. Compound-Specific Amino Acid Isotopic Analysis of Benthic Food Webs in the Chukchi Sea

    NASA Astrophysics Data System (ADS)

    Zhang, M.; Cooper, L. W.; Biasatti, D. M.; Grebmeier, J. M.

    2014-12-01

    The Chukchi Sea is known for locally high standing stocks of benthic macrofauna and strong coupling between pelagic-benthic components of the ecosystem. However, benthic food structure is not fully understood, due to varied sources of particulate organic matter (POM) and the high diversity of benthic invertebrates. We provide the first demonstration of the application of compound-specific amino acid isotope analysis to study the dietary sources and trophic structure for this Arctic marginal sea. About 20 stations in Chukchi Sea were sampled during cruises in August of 2012 and 2013. At each station, phytoplankton, POM and benthic fauna were collected, processed and analyzed using GC-C-IRMS (gas chromatography-combustion-isotope ratio mass spectrometry). Among benthic fauna, dominant species included the following taxonomic groups: Ophiuroidea, Amphipoda, Polychaeta, Gastropoda, Bivalvia, and Cnidaria. The benthic fauna showed similar patterns of individual amino acid δ13C, with glycine the most enriched in 13C and leucine the most depleted in 13C. Specific amino acids including phenylalanine showed spatial variability in δ13C and δ15N values within the sampled area, indicating contributions of different dietary sources including phytoplankton, sea ice algae, benthic algae and terrestrial organic materials. δ15N values of individual amino acids such as the difference between glutamic acid and phenylalanine, i.e. Δ15Nglu-phe (δ15Nglu - δ15Nphe), were also used to identify trophic levels of benthic invertebrates relative to estimates available from bulk δ15N values. These data will ultimately be used to evaluate the spatial variability of organic carbon sources and trophic level interactions of dominant benthic species in the Chukchi Sea.

  1. Aspartic acid racemization and amino acid composition of the organic endoskeleton of the deep-water colonial anemone Gerardia : Determination of longevity from kinetic experiments

    NASA Astrophysics Data System (ADS)

    Goodfriend, Glenn A.

    1997-05-01

    An age of 250 (±70) yr is estimated for a specimen of the colonial anemone Gerardia collected alive from 630 m depth off the Bahamas. This age is calculated from (1) the difference in aspartic acid (Asp) racemization measured in the outermost (youngest) and innermost (oldest) layers of the trunk of the organic endoskeleton of the specimen and (2) the estimated rate of Asp racenlization, as extrapolated from heating experiments. A progressive increase in D/L Asp values was observed from the outermost (0.052) to the innermost (0.099) layer of the trunk. Asp racemization rates determined at 60, 80, and 100°C in the laboratory were used to determine the Arrhenius parameters, from which the rate at present ambient temperature (12.5°C) was calculated. The activation energy of Asp racemization was estimated to be 29.1 kcal/mol. The estimated age (250 yr) is much younger than that suggested by radiocarbon measurements of the specimen (ca. 1800 yr). The amino acid composition of the endoskeleton of Gerardia is unusual, with histidine comprising 30% of the total. The composition resembles proteins of the endoskeleton of antipatharians (black corals).

  2. Ross Sea

    Atmospheric Science Data Center

    2013-04-16

    ... (3794 meters). It overlooks McMurdo Station, a U.S. research facility located near the tip of the island's Hut Point Peninsula. MISR was ... 2000 Images:  Ross Sea location:  Antarctica thumbnail:  ...

  3. Red Sea

    Atmospheric Science Data Center

    2013-04-16

    ... and is surrounded by hot, dry deserts and steppes. More fish species (over 1000) live in the Red Sea than in any other body of water the same size. Data: August 13, 2000; MISR Level 1B2 Ellipsoid ...

  4. Arabian Sea

    Atmospheric Science Data Center

    2013-04-16

    ... sometimes results in copious phytoplankton production and oxygen depletion of the subsurface waters. Although red phytoplankton fluorescences have been associated with the low oxygen concentrations in the intermediate and deep waters of the Arabian Sea, ...

  5. Sea Legs

    NASA Astrophysics Data System (ADS)

    Macdonald, Kenneth C.

    Forty-foot, storm-swept seas, Spitzbergen polar bears roaming vast expanses of Arctic ice, furtive exchanges of forbidden manuscripts in Cold War Moscow, the New York city fashion scene, diving in mini-subs to the sea floor hot srings, life with the astronauts, romance and heartbreak, and invading the last bastions of male exclusivity: all are present in this fast-moving, non-fiction account of one woman' fascinating adventures in the world of marine geology and oceanography.

  6. A Vertical Wall Dominated by Acesta excavata and Neopycnodonte zibrowii, Part of an Undersampled Group of Deep-Sea Habitats

    PubMed Central

    Johnson, Mark P.; White, Martin; Wilson, Annette; Würzberg, Laura; Schwabe, Enrico; Folch, Helka; Allcock, A. Louise

    2013-01-01

    We describe a novel biotope at 633 to 762 m depth on a vertical wall in the Whittard Canyon, an extensive canyon system reaching from the shelf to the deep sea on Ireland’s continental margin. We explored this wall with an ROV and compiled a photomosaic of the habitat. The assemblage contributing to the biotope was dominated by large limid bivalves, Acesta excavata (mean shell height 10.4 cm), and deep-sea oysters, Neopycnodonte zibrowii, at high densities, particularly at overhangs. Mean density of N. zibrowii increased with depth, with densities of the most closely packed areas of A. excavata also increasing with depth. Other taxa associated with the assemblage included the solitary coral Desmophyllum dianthus, cerianthid anemones, comatulid crinoids, the trochid gastropod Margarites sp., the portunid crab Bathynectes longispina and small fish of the family Bythitidae. The scleractinian coral Madrepora oculata, the pencil urchin Cidaris cidaris and a species of Epizoanthus were also common. Prominent but less abundant species included the flytrap anemone Actinoscyphia saginata, the carrier crab Paramola cuvieri, and the fishes Lepidion eques and Conger conger. Observations of the hydrography of the canyon system identified that the upper 500 m was dominated by Eastern North Atlantic Water, with Mediterranean Outflow Water beneath it. The permanent thermocline is found between 600 and 1000 m depth, i.e., in the depth range of the vertical wall and the dense assemblage of filter feeders. Beam attenuation indicated nepheloid layers present in the canyon system with the greatest amounts of suspended material at the ROV dive site between 500 and 750 m. A cross-canyon CTD transect indicated the presence of internal waves between these depths. We hypothesise that internal waves concentrate suspended sediment at high concentrations at the foot of the vertical wall, possibly explaining the large size and high density of filter-feeding molluscs. PMID:24260319

  7. [Land and marine fauna constituting a threat for recreational divers in the tropics].

    PubMed

    Korzeniewski, Krzysztof

    2008-09-01

    Due to intensively growing international tourism, increasing numbers of people leave for countries with hot climates, where various threats for human health and life exist. Besides climatic and sanitary conditions, a rich fauna, represented by predators and venomous animals, can be included. Based on available world literature and their own observations, the authors present the threats that a tourist can possibly encounter whilst relaxing on the beach or during recreational diving in tropical waters. When staying in water, a large threat is posed by marine fish of prey (sharks, barracuda, muraena), Cnidaria (jellyfish, corals, anemones) and venomous animals (fish, sea snakes). On land, on the other hand, a threat can be posed by venomous arthropods (scorpions, spiders) and Hymenoptera insects. The study presents the most important representatives of fauna present in coastal areas frequently visited by diving enthusiasts. Also, clinical image and conduct in the case of body injures are discussed. PMID:19112854

  8. Methodological approaches for nanotoxicology using cnidarian models.

    PubMed

    Ambrosone, Alfredo; Tortiglione, Claudia

    2013-03-01

    The remarkable amenability of aquatic invertebrates to laboratory manipulation has already made a few species belonging to the phylum Cnidaria as attracting systems for exploring animal development. The proliferation of molecular and genomic tools, including the whole genomic sequence of the freshwater polyp Hydra vulgaris and the starlet sea anemone Nematostella vectensis, further enhances the promise of these species to investigate the evolution of key aspects of development biology. In addition, the facility with which cnidarian population can be investigated within their natural ecological context suggests that these models may be profitably expanded to address important questions in ecology and toxicology. In this review, we explore the traits that make Hydra and Nematostella exceptionally attractive model organisms in context of nanotoxicology, and highlight a number of methods and developments likely to further increase that utility in the near future. PMID:23193991

  9. Mini-review: the evolution of neuropeptide signaling.

    PubMed

    Grimmelikhuijzen, Cornelis J P; Hauser, Frank

    2012-08-10

    Neuropeptides and their G protein-coupled receptors (GPCRs) have an early evolutionary origin and are already abundant in basal animals with primitive nervous systems such as cnidarians (Hydra, jellyfishes, corals, and sea anemones). Most animals emerging after the Cnidaria belong to two evolutionary lineages, the Protostomia (to which the majority of invertebrates belong) and Deuterostomia (to which some minor groups of invertebrates, and all vertebrates belong). These two lineages split about 700 million years (Myr) ago. Many mammalian neuropeptide GPCRs have orthologues in the Protostomia and this is also true for some of the mammalian neuropeptides. Examples are oxytocin/vasopressin, GnRH, gastrin/CCK, and neuropeptide Y and their GPCRs. These results implicate that protostomes (for example insects and nematodes) can be used as models to study the biology of neuropeptide signaling. PMID:22726357

  10. Surficial geology of the sea floor in Long Island Sound offshore of Orient Point, New York

    USGS Publications Warehouse

    McMullen, K.Y.; Poppe, L.J.; Danforth, W.W.; Blackwood, D.S.; Schaer, J.D.; Guberski, M.R.; Wood, D.A.; Doran, E.F.

    2011-01-01

    The U.S. Geological Survey (USGS), the National Oceanic and Atmospheric Administration (NOAA), and the Connecticut Department of Environmental Protection (CT DEP) are working cooperatively to map and interpret features of the sea floor along the northeastern coast of the United States. This report presents multibeam bathymetry and sidescan-sonar data obtained during NOAA survey H11446, which was conducted in a 12-km2 area in Long Island Sound offshore of Orient Point, NY. In addition, sediment and photographic data from 26 stations obtained during a USGS verification cruise are presented. Overall, the sea floor slopes gently seaward, but topography is more complex in sand-wave and boulder areas, which are evident in the multibeam and sidescan-sonar data from the study area. Sand waves generally have north-south-oriented crests with 10- to 20-m wavelengths. Sand-wave asymmetry indicates eastward net sediment transport in the east and westward net sediment transport in the northern and western parts of the study area. Areas with boulders on the sea floor are typically hummocky and are part of a glacial moraine system. Boulders are typically encrusted with seaweed, sponges, and anemones as shown in the bottom photography.

  11. Aral Sea

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This series of MODIS images shows the dwindling Aral Sea. Once one of the world's largest freshwater lakes, the Aral Sea has decreased by as much as 60% over the past few decades due to diversion of the water to grow cotton and rice. These diversion have dropped the lake levels, increased salinity, and nearly decimated the fishing industry. The previous extent of the lake is clearly visible as a whitish perimeter in these image from April 16, May 18, and June 3, 2002. s. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  12. Sea Ice

    NASA Technical Reports Server (NTRS)

    Perovich, D.; Gerland, S.; Hendricks, S.; Meier, Walter N.; Nicolaus, M.; Richter-Menge, J.; Tschudi, M.

    2013-01-01

    During 2013, Arctic sea ice extent remained well below normal, but the September 2013 minimum extent was substantially higher than the record-breaking minimum in 2012. Nonetheless, the minimum was still much lower than normal and the long-term trend Arctic September extent is -13.7 per decade relative to the 1981-2010 average. The less extreme conditions this year compared to 2012 were due to cooler temperatures and wind patterns that favored retention of ice through the summer. Sea ice thickness and volume remained near record-low levels, though indications are of slightly thicker ice compared to the record low of 2012.

  13. Sea ice in the China Sea

    SciTech Connect

    Deng Shuqi

    1993-12-31

    In every winter, sea ice occurring in Bohai Sea and the North Yellow Sea is the first-year ice which is going through generating, developing and thawing processes. Therefore, it is necessary to spatially and temporally describe ice period, freezing range, thickness variations and general motion of sea ice. The purpose of this paper is to provide initial general situation and features of sea ice for forecasting and researching sea ice.

  14. NUMERICAL INVESTIGATION OF A CORONAL MASS EJECTION FROM AN ANEMONE ACTIVE REGION: RECONNECTION AND DEFLECTION OF THE 2005 AUGUST 22 ERUPTION

    SciTech Connect

    Lugaz, N.; Shibata, K.; Downs, C.; Roussev, I. I.; Asai, A.; Gombosi, T. I.

    2011-09-10

    We present a numerical investigation of the coronal evolution of a coronal mass ejection (CME) on 2005 August 22 using a three-dimensional thermodynamic magnetohydrodynamic model, the space weather modeling framework. The source region of the eruption was anemone active region (AR) 10798, which emerged inside a coronal hole. We validate our modeled corona by producing synthetic extreme-ultraviolet (EUV) images, which we compare to EIT images. We initiate the CME with an out-of-equilibrium flux rope with an orientation and chirality chosen in agreement with observations of an H{alpha} filament. During the eruption, one footpoint of the flux rope reconnects with streamer magnetic field lines and with open field lines from the adjacent coronal hole. It yields an eruption which has a mix of closed and open twisted field lines due to interchange reconnection and only one footpoint line-tied to the source region. Even with the large-scale reconnection, we find no evidence of strong rotation of the CME as it propagates. We study the CME deflection and find that the effect of the Lorentz force is a deflection of the CME by about 3{sup 0} R{sup -1}{sub sun} toward the east during the first 30 minutes of the propagation. We also produce coronagraphic and EUV images of the CME, which we compare with real images, identifying a dimming region associated with the reconnection process. We discuss the implication of our results for the arrival at Earth of CMEs originating from the limb and for models to explain the presence of open field lines in magnetic clouds.

  15. Tree litter and forest understorey vegetation: a conceptual framework to understand the effects of tree litter on a perennial geophyte, Anemone nemorosa

    PubMed Central

    Baltzinger, Marie; Archaux, Frédéric; Dumas, Yann

    2012-01-01

    Background and Aims Litter is a key factor in structuring plant populations, through positive or negative interactions. The litter layer forms a mechanical barrier that is often strongly selective against individuals lacking hypocotyle plasticity. Litter composition also interacts with plant growth by providing beneficial nutrients or, inversely, by allowing harmful allelopathic leaching. As conspicuous litter fall accumulation is often observed under deciduous forests, interactions between tree litter and understorey plant populations are worthy of study. Methods In a 1-year ex-situ experiment, the effects of tree litter on the growth of Anemone nemorosa, a small perennial forest geophyte, were investigated. Three ‘litter quantity’ treatments were defined, representative of forest floor litter (199, 356·5 and 514 g m?2), which were crossed with five ‘litter composition’ treatments (Quercus petraea, Fagus sylvatica, Carpinus betulus, Q. petraea + F. sylvatica and Q. petraea + C. betulus), plus a no-litter control. Path analysis was then used to investigate the pathways linking litter characteristics and components of adult plant growth. Key Results As expected, the heavier the litter, the longer the petiole; rhizome growth, however, was not depreciated by the litter-induced petiole lengthening. Both rhizome mass increment and number of initiated buds marginally increased with the amount of litter. Rhizome mass increment was in fact determined primarily by leaf area and leaf life span, neither of which was unequivocally correlated with any litter characteristics. However, the presence of litter significantly increased leafing success: following a late frost event, control rhizomes growing in the absence of litter experienced higher leaf mortality before leaf unfolding. Conclusions The study questions the role of litter as a physical or chemical barrier to ground vegetation; to better understand this role, there is a need for ex-situ, longer-term experiments coupled with in-situ observations in the forest. PMID:22419760

  16. Calibration of stable oxygen isotopes in Siderastrea radians (Cnidaria:Scleractinia): Implications for slow-growing corals

    NASA Astrophysics Data System (ADS)

    Moses, Christopher S.; Swart, Peter K.; Dodge, Richard E.

    2006-09-01

    Geochemical proxies in the skeletons of corals used for the purpose of reconstructing environmental records have typically been obtained from relatively fast-growing corals (usually >8 mm yr-1) and from only a few key genera (most commonly Porites and Montastraea). In many areas, however, there are no suitable fast-growing corals available for such reconstructions. Here, we investigate the potential of Siderastrea radians, a slow-growing Atlantic and Caribbean zooxanthellate coral, as an archive of sea surface temperature (SST) and salinity over the period from 1891 to 2002. Sampling the skeleton of three corals from the Cape Verde Islands, we were able to reproduce a clear seasonal signal, but with limited correlation to monthly SST, arising from inadequate chronologic constraint of the individual samples. The SST-δ18O calibration slopes for different sampling scales on several cores can range from about -9°C ‰-1 to +2°C ‰-1 (compared to other published values of around -5 to -4°C ‰-1). Careful treatment produced a δ18O-SST calibration equation where SST(°C) = 12.56(±1.20) - 3.86(±0.39)*(δc-δw). The recognition of the limitations of calibration at such small growth rates due to skeletal complexity and suspicion of environmental interferences suggests the need for careful consideration in the interpretation of climate proxy results from S. radians and other slow-growing corals.

  17. First Evidence of Inbreeding, Relatedness and Chaotic Genetic Patchiness in the Holoplanktonic Jellyfish Pelagia noctiluca (Scyphozoa, Cnidaria)

    PubMed Central

    Aglieri, Giorgio; Papetti, Chiara; Zane, Lorenzo; Milisenda, Giacomo; Boero, Ferdinando; Piraino, Stefano

    2014-01-01

    Genetic drift and non-random mating seldom influence species with large breeding populations and high dispersal potential, characterized by unstructured gene pool and panmixia at a scale lower than the minimum dispersal range of individuals. In the present study, a set of nine microsatellite markers was developed and used to investigate the spatio-temporal genetic patterns of the holoplanktonic jellyfish Pelagia noctiluca (Scyphozoa) in the Southern Tyrrhenian Sea. Homozygote excess was detected at eight loci, and individuals exhibited intra-population relatedness higher than expected by chance in at least three samples. This result was supported by the presence of siblings in at least 5 out 8 samples, 4 of which contained full-sib in addition to half-sib dyads. Having tested and ruled out alternative explanations as null alleles, our results suggest the influence of reproductive and behavioural features in shaping the genetic structure of P. noctiluca, as outcomes of population genetics analyses pointed out. Indeed, the genetic differentiation among populations was globally small but highlighted: a) a spatial genetic patchiness uncorrelated with distance between sampling locations, and b) a significant genetic heterogeneity between samples collected in the same locations in different years. Therefore, despite its extreme dispersal potential, P. noctiluca does not maintain a single homogenous population, but rather these jellyfish appear to have intra-bloom localized recruitment and/or individual cohesiveness, whereby siblings more likely swarm together as a single group and remain close after spawning events. These findings provide the first evidence of family structures and consequent genetic patchiness in a species with highly dispersive potential throughout its whole life cycle, contributing to understanding the patterns of dispersal and connectivity in marine environments. PMID:24977703

  18. From Sea to Shining Sea

    ERIC Educational Resources Information Center

    Scott, Beverly

    2005-01-01

    Deep down in the depths of the sea, beautiful fish, mysterious ocean life, and unusual plants glimmer and glow in the eerie atmosphere of an ever-changing ocean. This article describes how, with this vision and a purpose in mind, three teachers pulled open classroom walls and joined forces so their second graders could create a mammoth 30 x 75"…

  19. From Sea to Shining Sea

    ERIC Educational Resources Information Center

    Scott, Beverly

    2005-01-01

    Deep down in the depths of the sea, beautiful fish, mysterious ocean life, and unusual plants glimmer and glow in the eerie atmosphere of an ever-changing ocean. This article describes how, with this vision and a purpose in mind, three teachers pulled open classroom walls and joined forces so their second graders could create a mammoth 30 x 75"…

  20. Climate-mediated changes in zooplankton community structure for the eastern Bering Sea

    NASA Astrophysics Data System (ADS)

    Eisner, Lisa B.; Napp, Jeffrey M.; Mier, Kathryn L.; Pinchuk, Alexei I.; Andrews, Alexander G.

    2014-11-01

    Zooplankton are critical to energy transfer between higher and lower trophic levels in the eastern Bering Sea ecosystem. Previous studies from the southeastern Bering Sea shelf documented substantial differences in zooplankton taxa in the Middle and Inner Shelf Domains between warm and cold years. Our investigation expands this analysis into the northern Bering Sea and the south Outer Domain, looking at zooplankton community structure during a period of climate-mediated, large-scale change. Elevated air temperatures in the early 2000s resulted in regional warming and low sea-ice extent in the southern shelf whereas the late 2000s were characterized by cold winters, extensive spring sea ice, and a well-developed pool of cold water over the entire Middle Domain. The abundance of large zooplankton taxa such as Calanus spp. (C. marshallae and C. glacialis), and Parasagitta elegans, increased from warm to cold periods, while the abundance of gelatinous zooplankton (Cnidaria) and small taxa decreased. Biomass followed the same trends as abundance, except that the biomass of small taxa in the southeastern Bering Sea remained constant due to changes in abundance of small copepod taxa (increases in Acartia spp. and Pseudocalanus spp. and decreases in Oithona spp.). Statistically significant changes in zooplankton community structure and individual species were greatest in the Middle Domain, but were evident in all shelf domains, and in both the northern and southern portions of the eastern shelf. Changes in community structure did not occur abruptly during the transition from warm to cold, but seemed to begin gradually and build as the influence of the sea ice and cold water temperatures persisted. The change occurred one year earlier in the northern than the southern Middle Shelf. These and previous observations demonstrate that lower trophic levels within the eastern Bering Sea respond to climate-mediated changes on a variety of time scales, including those shorter than the commonly accepted quasi-decadal time periods. This lack of resilience or inertia at the lowest trophic levels affects production at higher trophic levels and must be considered in management strategy evaluations of living marine resources.

  1. Benthic meiofaunal composition and community structure in the Sethukuda mangrove area and adjacent open sea, East coast of India

    NASA Astrophysics Data System (ADS)

    Thilagavathi, Balasubramanaian; Das, Bandana; Saravanakumar, Ayyappan; Raja, Kuzhanthaivel

    2011-06-01

    The ecological aspects of meiofaunal communities in the Muthupettai mangrove forest, East coast of India, has not been investigated in the last two decades. Surface water temperature ranged from 23.5 °C to 31.8 °C. Salinity varied from 24 to 34 ppt, while water pH fluctuated from 7.4 to 8.3. Dissolved oxygen concentration ranged from 3.86 to 5.33 mg/l. Meiofauna analysis in this study identified a total of 106 species from the mangrove and adjacent open sea area of Sethukuda. Among these, 56 species of foraminiferans, 20 species of nematodes, 7 species of harpacticoid copepods, 4 species of ostrocodes, and 2 species of rotifers were identified. Furthermore, a single species was identified from the following groups: ciliophora, cnidaria, gnathostomulida, insecta, propulida, bryozoa and polychaete larvae. Meiofaunal density varied between 12029 to 23493 individuals 10 cm/m2. The diversity index ranged from 3.515 to 3.680, species richness index varied from 6.384 to 8.497, and evenness index varied from 0.839 to 0876 in the mangrove area and adjacent open sea.

  2. Metazoan meiofauna in deep-sea canyons and adjacent open slopes: A large-scale comparison with focus on the rare taxa

    NASA Astrophysics Data System (ADS)

    Bianchelli, S.; Gambi, C.; Zeppilli, D.; Danovaro, R.

    2010-03-01

    Metazoan meiofaunal abundance, total biomass, nematode size and the richness of taxa were investigated along bathymetric gradients (from the shelf break down to ca. 5000-m depth) in six submarine canyons and on five adjacent open slopes of three deep-sea regions. The investigated areas were distributed along >2500 km, on the Portuguese to the Catalan and South Adriatic margins. The Portuguese and Catalan margins displayed the highest abundances, biomass and richness of taxa, while the lowest values were observed in the Central Mediterranean Sea. The comparison between canyons and the nearby open slopes showed the lack of significant differences in terms of meiofaunal abundance and biomass at any sampling depth. In most canyons and on most slopes, meiofaunal variables did not display consistent bathymetric patterns. Conversely, we found that the different topographic features were apparently responsible for significant differences in the abundance and distribution of the rare meiofaunal taxa (i.e. taxa accounting for <1% of total meiofaunal abundance). Several taxa belonging to the temporary meiofauna, such as larvae/juveniles of Priapulida, Holothuroidea, Ascidiacea and Cnidaria, were encountered exclusively on open slopes, while others (including the Tanaidacea and Echinodea larvae) were found exclusively in canyons sediments. Results reported here indicate that, at large spatial scales, differences in deep-sea meiofaunal abundance and biomass are not only controlled by the available food sources, but also by the region or habitat specific topographic features, which apparently play a key role in the distribution of rare benthic taxa.

  3. The macrofauna of a stony sand area in the German Bight (North Sea)

    NASA Astrophysics Data System (ADS)

    Kühne, S.; Rachor, E.

    1996-12-01

    The sublittoral macrofauna of the Steingrund, a stony area east-northeast of Helgoland, was investigated from May till October 1991 using a van Veen grab and a small dredge. The diverse endo- and epifauna of the sandy bottoms, pebbles and boulders of this Saalian end moraine comprised 289 taxa, whereby the polychaetes Magelona papillicornis, Lanice conchilega and Spiophanes bombyx dominated in terms of abundance. Species rare in the German Bight, such as the sponge Leucandra fistulosa, the sea urchin Echinus esculentus, and the sea anemone Haliplanella lineata, were also found. The two sample sets were processed separately with multivariate techniques, and differentiated on the basis of occurrence and numbers of the abundant species. The analysis of the grab samples revealed two types of the Tellina-fabula-community. These associations were differentiated by the presence of species of the coarse-sand-inhabiting Goniadella-Spisula-community and were related to the distribution of the grain size of the sediment. Likewise, two epifaunal assemblages were distinguished. Sandy bottoms were characterized by Ophiura albida, Liocarcinus holsatus and Pagurus bernhardus, while boulders and pebbles were covered by a varied sessile and mobile epifauna dominated by the sessile bryozoan Flustra foliacea and the mobile pantopode Aechelia echinata. Numerical density, biomass and annual production estimates are in the range of values determined for the macrobenthos of the German Bight, while annual P/B ratios mounted up to 5.

  4. Evolution and functional diversity of jellyfish opsins.

    PubMed

    Suga, Hiroshi; Schmid, Volker; Gehring, Walter J

    2008-01-01

    Cnidaria are the most basal animal phylum possessing complex eyes [1]. Their eyes predominantly use ciliary photoreceptor cells (c-PRCs) like vertebrates, whereas insect eyes use rhabdomeric photoreceptor cells (r-PRCs) [1-4]. These two cell types show not only different cytoarchitectures but distinct phototransduction cascades, which are triggered by the respective types of opsins (e.g., [5]), ciliary opsins (c-opsins) and rhabdomeric opsins (r-opsins) [6]. Recent reports suggested that the c- and r-PRCs and their respective opsins diverged at least before the deuterostome-protostome split [7-9]. To study the earlier evolution of animal PRCs and opsins, we investigated two hydrozoan jellyfishes. We report here the first-characterized cnidarian opsins. Molecular phylogeny revealed that the cloned 20 jellyfish opsins, together with all the opsins from a hydra and some from a sea anemone, are more closely related to the c-opsins than to any other major opsin subfamily, indicating that the divergence of c- and r-opsins antedates the Cnidaria-Bilateria split. Possible scenarios of animal PRC evolution are discussed. Furthermore, Cladonema opsins show several distinct tissue- and stage-specific expression patterns. The expression of specific opsins in the eyes suggests a role in vision, whereas that in the gonads suggests a role in light-controlled release of gametes. PMID:18160295

  5. Surficial geology of the sea floor in Central Rhode Island Sound Southeast of Point Judith, Rhode Island

    USGS Publications Warehouse

    McMullen, K.Y.; Poppe, L.J.; Ackerman, S.D.; Blackwood, D.S.; Schaer, J.D.; Nadeau, M.A.; Wood, D.A.

    2011-01-01

    The U.S. Geological Survey (USGS) and the National Oceanic and Atmospheric Administration (NOAA) are working together to study sea-floor environments off the northeast coast of the United States. During 2008, NOAA survey H11996 collected multibeam echosounder data in a 65-square kilometer area in central Rhode Island Sound, southeast of Point Judith, Rhode Island. During 2010, the USGS collected bottom photographs and sediment samples from 25 stations in this study area. The bathymetry, photography, and sediment data are used to interpret sea-floor features including scour depressions, sand waves, trawl marks, and dredge spoils. Scour depressions cover the bathymetric highs in much of the study area. Sand waves are located mostly in the southwest, and trawl marks tend to be in the northern regions. Dredge spoils are located at a disposal site in a bathymetric low in the western end of the study area. Most stations have a sea-floor surface of sand or silty sand, but eight of the stations have boulders to pea-sized gravel or gravelly sediment on the surface. Photographs show sandy areas typically have scattered burrows, shells, amphipod communities, and worm tubes. Boulders and cobbles are commonly overgrown with hydrozoans and anemones.

  6. Sea Ice

    NASA Technical Reports Server (NTRS)

    Parkinson, Claire L.; Cavalieri, Donald J.

    2005-01-01

    Sea ice covers vast areas of the polar oceans, with ice extent in the Northern Hemisphere ranging from approximately 7 x 10(exp 6) sq km in September to approximately 15 x 10(exp 6) sq km in March and ice extent in the Southern Hemisphere ranging from approximately 3 x 10(exp 6) sq km in February to approximately 18 x 10(exp 6) sq km in September. These ice covers have major impacts on the atmosphere, oceans, and ecosystems of the polar regions, and so as changes occur in them there are potential widespread consequences. Satellite data reveal considerable interannual variability in both polar sea ice covers, and many studies suggest possible connections between the ice and various oscillations within the climate system, such as the Arctic Oscillation, North Atlantic Oscillation, and Antarctic Oscillation, or Southern Annular Mode. Nonetheless, statistically significant long-term trends are also apparent, including overall trends of decreased ice coverage in the Arctic and increased ice coverage in the Antarctic from late 1978 through the end of 2003, with the Antarctic ice increases following marked decreases in the Antarctic ice during the 1970s. For a detailed picture of the seasonally varying ice cover at the start of the 21st century, this chapter includes ice concentration maps for each month of 2001 for both the Arctic and the Antarctic, as well as an overview of what the satellite record has revealed about the two polar ice covers from the 1970s through 2003.

  7. Evolution of the tetraploid Anemone multifida (2n = 32) and hexaploid A. baldensis (2n = 48) (Ranunculaceae) was accompanied by rDNA loci loss and intergenomic translocation: evidence for their common genome origin

    PubMed Central

    Mlinarec, J.; Šatovi?, Z.; Malenica, N.; Ivan?i?-Ba?e, I.; Besendorfer, V.

    2012-01-01

    Background and Aims In the genus Anemone two small groups of taxa occur with the highest ploidy levels 2n = 6x = 48, belonging to the closely related clades: the montane/alpine Baldensis clade and the more temperate Multifida clade. To understand the formation of polyploids within these groups, the evolution of allohexaploid A. baldensis (AABBDD, 2n = 6x = 48) from Europe and allotetraploid Anemone multifida (BBDD, 2n = 4x = 32) from America was analysed. Methods Internal transcribed spacer and non-transcribed spacer sequences were used as molecular markers for phylogenetic analyses. Cytogenetic studies, including genomic in situ hybridization with genomic DNA of potential parental species as probe, fluorescence in situ hybridization with 5S and 18S rDNA as probes and 18S rDNA restriction analyses, were used to identify the parental origin of chromosomes and to study genomic changes following polyploidization. Key Results This study shows that A. multifida (BBDD, 2n= 4x = 32) and A. baldensis (AABBDD, 2n = 6x = 48) are allopolyploids originating from the crosses of diploid members of the Multifida (donor of the A and B subgenomes) and Baldensis groups (donor of the D subgenome). The A and B subgenomes are closely related to the genomes of A. sylvestris, A. virginiana and A. cylindrica, indicating that these species or their progeny might be the ancestral donors of the B subgenome of A. multifida and A and B subgenomes of A. baldensis. Both polyploids have undergone genomic changes such as interchromosomal translocation affecting B and D subgenomes and changes at rDNA sites. Anemone multifida has lost the 35S rDNA loci characteristic of the maternal donor (B subgenome) and maintained only the rDNA loci of the paternal donor (D subgenome). Conclusions It is proposed that A. multifida and A. baldensis probably had a common ancestor and their evolution was facilitated by vegetation changes during the Quaternary, resulting in their present disjunctive distribution. PMID:22711694

  8. Mammals of the Sea.

    ERIC Educational Resources Information Center

    Naturescope, 1986

    1986-01-01

    Presents information on sea mammals, including definitions and characteristics of cetaceans, pinnipeds, and sirenians. Contains descriptions of the teaching activities "Whale Music,""Draw A Whale to Scale,""Adopt a Sea Mammal," and "Sea Mammal Sleuths." (TW)

  9. Tracking Sea Otters

    USGS Multimedia Gallery

    USGS sea otter researcher Tim Tinker drives the boat on an expedition to track and observe sea otters in Monterey Bay, California. USGS scientists study sea otters in efforts to help the threatened species continue to recover from near extinction....

  10. Studying Sea Otters

    USGS Multimedia Gallery

    Sea otter researcher Michelle Staedler, of the Monterey Bay Aquarium, records sea otter behavior in her logbook as part of a study with the USGS and the University of California at Santa Cruz on sea otter behavior. ...

  11. The discovery of new deep-sea hydrothermal vent communities in the southern ocean and implications for biogeography.

    PubMed

    Rogers, Alex D; Tyler, Paul A; Connelly, Douglas P; Copley, Jon T; James, Rachael; Larter, Robert D; Linse, Katrin; Mills, Rachel A; Garabato, Alfredo Naveira; Pancost, Richard D; Pearce, David A; Polunin, Nicholas V C; German, Christopher R; Shank, Timothy; Boersch-Supan, Philipp H; Alker, Belinda J; Aquilina, Alfred; Bennett, Sarah A; Clarke, Andrew; Dinley, Robert J J; Graham, Alastair G C; Green, Darryl R H; Hawkes, Jeffrey A; Hepburn, Laura; Hilario, Ana; Huvenne, Veerle A I; Marsh, Leigh; Ramirez-Llodra, Eva; Reid, William D K; Roterman, Christopher N; Sweeting, Christopher J; Thatje, Sven; Zwirglmaier, Katrin

    2012-01-01

    Since the first discovery of deep-sea hydrothermal vents along the Galápagos Rift in 1977, numerous vent sites and endemic faunal assemblages have been found along mid-ocean ridges and back-arc basins at low to mid latitudes. These discoveries have suggested the existence of separate biogeographic provinces in the Atlantic and the North West Pacific, the existence of a province including the South West Pacific and Indian Ocean, and a separation of the North East Pacific, North East Pacific Rise, and South East Pacific Rise. The Southern Ocean is known to be a region of high deep-sea species diversity and centre of origin for the global deep-sea fauna. It has also been proposed as a gateway connecting hydrothermal vents in different oceans but is little explored because of extreme conditions. Since 2009 we have explored two segments of the East Scotia Ridge (ESR) in the Southern Ocean using a remotely operated vehicle. In each segment we located deep-sea hydrothermal vents hosting high-temperature black smokers up to 382.8°C and diffuse venting. The chemosynthetic ecosystems hosted by these vents are dominated by a new yeti crab (Kiwa n. sp.), stalked barnacles, limpets, peltospiroid gastropods, anemones, and a predatory sea star. Taxa abundant in vent ecosystems in other oceans, including polychaete worms (Siboglinidae), bathymodiolid mussels, and alvinocaridid shrimps, are absent from the ESR vents. These groups, except the Siboglinidae, possess planktotrophic larvae, rare in Antarctic marine invertebrates, suggesting that the environmental conditions of the Southern Ocean may act as a dispersal filter for vent taxa. Evidence from the distinctive fauna, the unique community structure, and multivariate analyses suggest that the Antarctic vent ecosystems represent a new vent biogeographic province. However, multivariate analyses of species present at the ESR and at other deep-sea hydrothermal vents globally indicate that vent biogeography is more complex than previously recognised. PMID:22235194

  12. The Discovery of New Deep-Sea Hydrothermal Vent Communities in the Southern Ocean and Implications for Biogeography

    PubMed Central

    Rogers, Alex D.; Tyler, Paul A.; Connelly, Douglas P.; Copley, Jon T.; James, Rachael; Larter, Robert D.; Linse, Katrin; Mills, Rachel A.; Garabato, Alfredo Naveira; Pancost, Richard D.; Pearce, David A.; Polunin, Nicholas V. C.; German, Christopher R.; Shank, Timothy; Boersch-Supan, Philipp H.; Alker, Belinda J.; Aquilina, Alfred; Bennett, Sarah A.; Clarke, Andrew; Dinley, Robert J. J.; Graham, Alastair G. C.; Green, Darryl R. H.; Hawkes, Jeffrey A.; Hepburn, Laura; Hilario, Ana; Huvenne, Veerle A. I.; Marsh, Leigh; Ramirez-Llodra, Eva; Reid, William D. K.; Roterman, Christopher N.; Sweeting, Christopher J.; Thatje, Sven; Zwirglmaier, Katrin

    2012-01-01

    Since the first discovery of deep-sea hydrothermal vents along the Galápagos Rift in 1977, numerous vent sites and endemic faunal assemblages have been found along mid-ocean ridges and back-arc basins at low to mid latitudes. These discoveries have suggested the existence of separate biogeographic provinces in the Atlantic and the North West Pacific, the existence of a province including the South West Pacific and Indian Ocean, and a separation of the North East Pacific, North East Pacific Rise, and South East Pacific Rise. The Southern Ocean is known to be a region of high deep-sea species diversity and centre of origin for the global deep-sea fauna. It has also been proposed as a gateway connecting hydrothermal vents in different oceans but is little explored because of extreme conditions. Since 2009 we have explored two segments of the East Scotia Ridge (ESR) in the Southern Ocean using a remotely operated vehicle. In each segment we located deep-sea hydrothermal vents hosting high-temperature black smokers up to 382.8°C and diffuse venting. The chemosynthetic ecosystems hosted by these vents are dominated by a new yeti crab (Kiwa n. sp.), stalked barnacles, limpets, peltospiroid gastropods, anemones, and a predatory sea star. Taxa abundant in vent ecosystems in other oceans, including polychaete worms (Siboglinidae), bathymodiolid mussels, and alvinocaridid shrimps, are absent from the ESR vents. These groups, except the Siboglinidae, possess planktotrophic larvae, rare in Antarctic marine invertebrates, suggesting that the environmental conditions of the Southern Ocean may act as a dispersal filter for vent taxa. Evidence from the distinctive fauna, the unique community structure, and multivariate analyses suggest that the Antarctic vent ecosystems represent a new vent biogeographic province. However, multivariate analyses of species present at the ESR and at other deep-sea hydrothermal vents globally indicate that vent biogeography is more complex than previously recognised. PMID:22235194

  13. Diversity, Distribution and Nature of Faunal Associations with Deep-Sea Pennatulacean Corals in the Northwest Atlantic

    PubMed Central

    Baillon, Sandrine; Hamel, Jean-François; Mercier, Annie

    2014-01-01

    Anthoptilum grandiflorum and Halipteris finmarchica are two deep-sea corals (Octocorallia: Pennatulacea) common on soft bottoms in the North Atlantic where they are believed to act as biogenic habitat. The former also has a worldwide distribution. To assist conservation efforts, this study examines spatial and temporal patterns in the abundance, diversity, and nature of their faunal associates. A total of 14 species were found on A. grandiflorum and 6 species on H. finmarchica during a multi-year and multi-site sampling campaign in eastern Canada. Among those, 7 and 5 species, respectively, were attached to the sea pens and categorized as close associates or symbionts. Rarefaction analyses suggest that the most common associates of both sea pens have been sampled. Biodiversity associated with each sea pen is analyzed according to season, depth and region using either close associates or the broader collection of species. Associated biodiversity generally increases from northern to southern locations and does not vary with depth (?100–1400 m). Seasonal patterns in A. grandiflorum show higher biodiversity during spring/summer due to the transient presence of early life stages of fishes and shrimps whereas it peaks in fall for H. finmarchica. Two distinct endoparasitic species of highly modified copepods (families Lamippidae and Corallovexiidae) commonly occur in the polyps of A. grandiflorum and H. finmarchica, and a commensal sea anemone frequently associates with H. finmarchica. Stable isotope analyses (?13C and ?15N) reveal potential trophic interactions between the parasites and their hosts. Overall, the diversity of obligate/permanent associates of sea pens is moderate; however the presence of mobile/transient associates highlights an ecological role that has yet to be fully elucidated and supports their key contribution to the enhancement of biodiversity in the Northwest Atlantic. PMID:25369515

  14. Regulation of intracellular pH in cnidarians: response to acidosis in Anemonia viridis.

    PubMed

    Laurent, Julien; Venn, Alexander; Tambutté, Éric; Ganot, Philippe; Allemand, Denis; Tambutté, Sylvie

    2014-02-01

    The regulation of intracellular pH (pHi) is a fundamental aspect of cell physiology that has received little attention in studies of the phylum Cnidaria, which includes ecologically important sea anemones and reef-building corals. Like all organisms, cnidarians must maintain pH homeostasis to counterbalance reductions in pHi, which can arise because of changes in either intrinsic or extrinsic parameters. Corals and sea anemones face natural daily changes in internal fluids, where the extracellular pH can range from 8.9 during the day to 7.4 at night. Furthermore, cnidarians are likely to experience future CO?-driven declines in seawater pH, a process known as ocean acidification. Here, we carried out the first mechanistic investigation to determine how cnidarian pHi regulation responds to decreases in extracellular and intracellular pH. Using the anemone Anemonia viridis, we employed confocal live cell imaging and a pH-sensitive dye to track the dynamics of pHi after intracellular acidosis induced by acute exposure to decreases in seawater pH and NH?Cl prepulses. The investigation was conducted on cells that contained intracellular symbiotic algae (Symbiodinium sp.) and on symbiont-free endoderm cells. Experiments using inhibitors and Na?-free seawater indicate a potential role of Na?/H? plasma membrane exchangers (NHEs) in mediating pHi recovery following intracellular acidosis in both cell types. We also measured the buffering capacity of cells, and obtained values between 20.8 and 43.8 mM per pH unit, which are comparable to those in other invertebrates. Our findings provide the first steps towards a better understanding of acid-base regulation in these basal metazoans, for which information on cell physiology is extremely limited. PMID:24256552

  15. The organizer in evolution-gastrulation and organizer gene expression highlight the importance of Brachyury during development of the coral, Acropora millepora.

    PubMed

    Hayward, David C; Grasso, Lauretta C; Saint, Robert; Miller, David J; Ball, Eldon E

    2015-03-15

    Organizer activity, once thought to be restricted to vertebrates, has ancient origins. However, among non-bilaterians, it has only been subjected to detailed investigation during embryonic development of the sea anemone, Nematostella vectensis. As a step toward establishing the extent to which findings in Nematostella can be generalized across the large and diverse phylum Cnidaria, we examined the expression of some key organizer and gastrulation genes during the embryonic development of the coral Acropora millepora. Although anemones and corals both belong to the cnidarian class Anthozoa, the two lineages diverged during the Cambrian and the morphological development of Acropora differs in several important respects from that of Nematostella. While the expression patterns of the key genes brachyury, bmp2/4, chordin, goosecoid and forkhead are broadly similar, developmental differences between the two species enable novel observations, and new interpretations of their significance. Specifically, brachyury expression during the flattened prawnchip stage before gastrulation, a developmental peculiarity of Acropora, leads us to suggest that it is the key gene demarcating ectoderm from endoderm in Acropora, and by implication in other cnidarians, whereas previous studies in Nematostella proposed that forkhead plays this role. Other novel observations include the transient expression of Acropora forkhead in scattered ectodermal cells shortly after gastrulation, and in the developing mesenterial filaments, with no corresponding expression reported in Nematostella. In addition, the expression patterns of goosecoid and bmp2/4 confirm the fundamental bilaterality of the Anthozoa. PMID:25601451

  16. Colorful Underwater Sea Creatures

    ERIC Educational Resources Information Center

    McCutcheon, Heather

    2011-01-01

    In this article, the author describes a project wherein students created colorful underwater sea creatures. This project began with a discussion about underwater sea creatures and how they live. The first step was making the multi-colored tissue paper that would become sea creatures and seaweed. Once students had the shapes of their sea creatures…

  17. Colorful Underwater Sea Creatures

    ERIC Educational Resources Information Center

    McCutcheon, Heather

    2011-01-01

    In this article, the author describes a project wherein students created colorful underwater sea creatures. This project began with a discussion about underwater sea creatures and how they live. The first step was making the multi-colored tissue paper that would become sea creatures and seaweed. Once students had the shapes of their sea creatures…

  18. Beaufort Sea: information update

    SciTech Connect

    Becker, P.R.

    1988-04-01

    The report is based on a multi-disciplinary meeting held March 6-7, 1985, as part of preparations for the Beaufort Sea Sale 97. The chapters are based on presentations given: The causeway effect: Modification of nearshore thermal regime resulting from causeways; Summertime sea ice intrusions in the Chukchi Sea; The deepwater limit of ice gouging on the Beaufort Sea shelf; Distribution, abundance, migration, harvest, and stock identity of Belukha Whales in the Beaufort Sea; Ringed seals in the Beaufort Sea; Beaufort Sea socioeconomics; The Baffin Island Oil Spill, (BIOS) Project.

  19. Age, Growth, Reproduction and Feeding of the Spurdog ( Squalus acanthias Linnaeus, 1758) in the South-eastern Black Sea

    NASA Astrophysics Data System (ADS)

    Avsar, D.

    2001-02-01

    Life-history parameters and diet of spurdog ( Squalus acanthias) sampled from the SE Black Sea were studied. Spurdog from age classes I to XIV were identified, with a dominance of age class VIII for both sexes. The length-weight relationship was W=0·0040*L 2·95and the mean annual growth rates in length and weight were 7·2 cm and 540·1 g, respectively. The estimated von Bertalanffy growth parameters were: W ?=12021 (g), L ?=157 (cm), K=0·12 (year -1) and t 0=-1·30 (year). The size at first maturity was 82 cm for males and 88 cm for females. Mean biennial fecundity was also found to be 8 pups/female. The relationships between fecundity-length, fecundity-weight and fecundity-age were found to be: F=-17·0842+0·2369*L (R=0·93), F=0·3780+0·0018*W (R=0·89) and F =-0·7859+1·1609*A (R=0·94) respectively. The spurdog can be considered an opportunistic feeder. Their natural diet was composed mainly of teleost fishes, followed by Crustaceans, Nematodes and Actinarians (=sea anemones). Whiting ( Merlangius merlangus euxinus) was the predominant prey item among their fish prey. Demersal teleosts formed the majority of the diet, and there was no difference ( P>0·05) among the food items of immature, maturing and mature individuals of both sexes.

  20. 7 CFR 1437.310 - Sea grass and sea oats.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false Sea grass and sea oats. 1437.310 Section 1437.310 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT... Determining Coverage Using Value § 1437.310 Sea grass and sea oats. (a) Sea grass and sea oats are value...

  1. 7 CFR 1437.310 - Sea grass and sea oats.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false Sea grass and sea oats. 1437.310 Section 1437.310 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT... Determining Coverage Using Value § 1437.310 Sea grass and sea oats. (a) Sea grass and sea oats are value...

  2. 7 CFR 1437.310 - Sea grass and sea oats.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false Sea grass and sea oats. 1437.310 Section 1437.310 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT... Determining Coverage Using Value § 1437.310 Sea grass and sea oats. (a) Sea grass and sea oats are value...

  3. Deep sea waste disposal

    SciTech Connect

    Kester, D.R.; Burt, W.V.; Capuzzo, J.M.; Park, P.K.; Ketchum, B.W.; Duedall, I.W.

    1985-01-01

    The book presents papers on the marine disposal of wastes. Topics considered include incineration at sea, the modelling and biological effects of industrial wastes, microbial studies of ocean dumping, deep-sea mining wastes, the chemical analysis of ferromanganese nodules, and economic aspects of deep-sea disposal.

  4. All That Unplowed Sea

    ERIC Educational Resources Information Center

    MOSAIC, 1975

    1975-01-01

    Hunting and gathering at sea may fast be approaching their productive limits. Aquaculture - farming at sea - linked to conservation represents the sea's promise. If the system works, it might prove to be the key to supplying large amounts of food and fresh water at no cost in nonrenewable energy resources. (BT)

  5. Tracking Sea Otters

    USGS Multimedia Gallery

    Sea otter researchers Michelle Staedler, Monterey Bay Aquarium, and Tim Tinker, USGS, work together to locate sea otters in their study project. USGS scientists and their partners study sea otters in efforts to help the threatened species continue to recover from near extinction....

  6. Regional sea level variability in the Bohai Sea, Yellow Sea, and East China Sea

    NASA Astrophysics Data System (ADS)

    Cheng, Yongcun; Plag, Hans-Peter; Hamlington, Benjamin D.; Xu, Qing; He, Yijun

    2015-12-01

    The regional sea level variability in the Bohai Sea (BS), Yellow Sea (YS) and East China Sea (ECS) is investigated based on tide gauge, satellite altimeter data and an independent oceanic general circulation model for the Earth Simulator (OFES) model outputs. It is found that atmospheric forcing significantly affects local sea level variability in the BS and YS and local sea level variability at the Southern ECS is highly correlated with along-shore currents. Particularly, the annual sea level fluctuations potentially change inundation risk and the frequency and magnitude of flooding in regions with high annual sea level. Hence, the cyclostationary empirical orthogonal function (CSEOF) analysis is carried out to investigate the variations of annual sea level cycle amplitude. Similar spatial distribution characteristics of annual sea level amplitude fluctuations are presented from satellite altimeter data and model outputs. The variability of annual sea level amplitude estimated from the satellite altimeter data agrees well with that from the tide gauge data, and positively (negatively) correlates with Southern Oscillation Index (Pacific Decadal Oscillation). The OFES model, however, underestimates the fluctuation of the annual cycle. After removing the annual signal, the low-passed (i.e., 13-month running mean) tide gauge data shows high correlations with SOI and PDO on time scales over 8 years in the BS and ECS.

  7. Dust Storm, Aral Sea

    NASA Technical Reports Server (NTRS)

    2002-01-01

    The Aral Sea has shrunk to less than half its size since 1985. The Aral Sea receives little water (sometimes no water) from the two major rivers that empty into it-the Syr Darya and Amu Darya. Instead, the river water is diverted to support irrigation for the region's extensive cotton fields. Recently, water scarcity has increased due to a prolonged drought in Central Asia. As the Aral Sea recedes, its former sea bed is exposed. The Aral's sea bed is composed of fine sediments-including fertilizers and other agricultural chemicals-that are easily picked up by the region's strong winds, creating thick dust storms. The International Space Station crew observed and recorded a large dust storm blowing eastward from the Aral Sea in late June 2001. This image illustrates the strong coupling between human activities (water diversions and irrigation), and rapidly changing land, sea and atmospheric processes-the winds blow across the

  8. Sea level extremes in the Caribbean Sea

    NASA Astrophysics Data System (ADS)

    Torres, R. Ricardo; Tsimplis, Michael N.

    2014-08-01

    Sea level extremes in the Caribbean Sea are analyzed on the basis of hourly records from 13 tide gauges. The largest sea level extreme observed is 83 cm at Port Spain. The largest nontidal residual in the records is 76 cm, forced by a category 5 hurricane. Storm surges in the Caribbean are primarily caused by tropical storms and stationary cold fronts intruding the basin. However, the seasonal signal and mesoscale eddies also contribute to the creation of extremes. The five stations that have more than 20 years of data show significant trends in the extremes suggesting that flooding events are expected to become more frequent in the future. The observed trends in extremes are caused by mean sea level rise. There is no evidence of secular changes in the storm activity. Sea level return periods have also been estimated. In the south Colombian Basin, where large hurricane-induced surges are rare, stable estimates can be obtained with 30 years of data or more. For the north of the basin, where large hurricane-induced surges are more frequent, at least 40 years of data are required. This suggests that the present data set is not sufficiently long for robust estimates of return periods. ENSO variability correlates with the nontidal extremes, indicating a reduction of the storm activity during positive ENSO events. The period with the highest extremes is around October, when the various sea level contributors' maxima coincide.

  9. East Siberian Sea, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    The winter sea ice in the east Siberian Sea is looking a bit like a cracked windshield in these true-color Moderate Resolution Imaging Spectroradiometer (MODIS) images from June 16 and 23, 2002. North of the thawing tundra, the sea ice takes on its cracked, bright blue appearance as it thins, which allows the reflection of the water to show through. Numerous still-frozen lakes dot the tundra. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  10. Semi-Automated Image Analysis for the Assessment of Megafaunal Densities at the Arctic Deep-Sea Observatory HAUSGARTEN

    PubMed Central

    Schoening, Timm; Bergmann, Melanie; Ontrup, Jörg; Taylor, James; Dannheim, Jennifer; Gutt, Julian; Purser, Autun; Nattkemper, Tim W.

    2012-01-01

    Megafauna play an important role in benthic ecosystem function and are sensitive indicators of environmental change. Non-invasive monitoring of benthic communities can be accomplished by seafloor imaging. However, manual quantification of megafauna in images is labor-intensive and therefore, this organism size class is often neglected in ecosystem studies. Automated image analysis has been proposed as a possible approach to such analysis, but the heterogeneity of megafaunal communities poses a non-trivial challenge for such automated techniques. Here, the potential of a generalized object detection architecture, referred to as iSIS (intelligent Screening of underwater Image Sequences), for the quantification of a heterogenous group of megafauna taxa is investigated. The iSIS system is tuned for a particular image sequence (i.e. a transect) using a small subset of the images, in which megafauna taxa positions were previously marked by an expert. To investigate the potential of iSIS and compare its results with those obtained from human experts, a group of eight different taxa from one camera transect of seafloor images taken at the Arctic deep-sea observatory HAUSGARTEN is used. The results show that inter- and intra-observer agreements of human experts exhibit considerable variation between the species, with a similar degree of variation apparent in the automatically derived results obtained by iSIS. Whilst some taxa (e. g. Bathycrinus stalks, Kolga hyalina, small white sea anemone) were well detected by iSIS (i. e. overall Sensitivity: 87%, overall Positive Predictive Value: 67%), some taxa such as the small sea cucumber Elpidia heckeri remain challenging, for both human observers and iSIS. PMID:22719868

  11. Semi-automated image analysis for the assessment of megafaunal densities at the Arctic deep-sea observatory HAUSGARTEN.

    PubMed

    Schoening, Timm; Bergmann, Melanie; Ontrup, Jörg; Taylor, James; Dannheim, Jennifer; Gutt, Julian; Purser, Autun; Nattkemper, Tim W

    2012-01-01

    Megafauna play an important role in benthic ecosystem function and are sensitive indicators of environmental change. Non-invasive monitoring of benthic communities can be accomplished by seafloor imaging. However, manual quantification of megafauna in images is labor-intensive and therefore, this organism size class is often neglected in ecosystem studies. Automated image analysis has been proposed as a possible approach to such analysis, but the heterogeneity of megafaunal communities poses a non-trivial challenge for such automated techniques. Here, the potential of a generalized object detection architecture, referred to as iSIS (intelligent Screening of underwater Image Sequences), for the quantification of a heterogenous group of megafauna taxa is investigated. The iSIS system is tuned for a particular image sequence (i.e. a transect) using a small subset of the images, in which megafauna taxa positions were previously marked by an expert. To investigate the potential of iSIS and compare its results with those obtained from human experts, a group of eight different taxa from one camera transect of seafloor images taken at the Arctic deep-sea observatory HAUSGARTEN is used. The results show that inter- and intra-observer agreements of human experts exhibit considerable variation between the species, with a similar degree of variation apparent in the automatically derived results obtained by iSIS. Whilst some taxa (e. g. Bathycrinus stalks, Kolga hyalina, small white sea anemone) were well detected by iSIS (i. e. overall Sensitivity: 87%, overall Positive Predictive Value: 67%), some taxa such as the small sea cucumber Elpidia heckeri remain challenging, for both human observers and iSIS. PMID:22719868

  12. Pelagic sea snakes dehydrate at sea.

    PubMed

    Lillywhite, Harvey B; Sheehy, Coleman M; Brischoux, François; Grech, Alana

    2014-05-01

    Secondarily marine vertebrates are thought to live independently of fresh water. Here, we demonstrate a paradigm shift for the widely distributed pelagic sea snake, Hydrophis (Pelamis) platurus, which dehydrates at sea and spends a significant part of its life in a dehydrated state corresponding to seasonal drought. Snakes that are captured following prolonged periods without rainfall have lower body water content, lower body condition and increased tendencies to drink fresh water than do snakes that are captured following seasonal periods of high rainfall. These animals do not drink seawater and must rehydrate by drinking from a freshwater lens that forms on the ocean surface during heavy precipitation. The new data based on field studies indicate unequivocally that this marine vertebrate dehydrates at sea where individuals may live in a dehydrated state for possibly six to seven months at a time. This information provides new insights for understanding water requirements of sea snakes, reasons for recent declines and extinctions of sea snakes and more accurate prediction for how changing patterns of precipitation might affect these and other secondarily marine vertebrates living in tropical oceans. PMID:24648228

  13. Pelagic sea snakes dehydrate at sea

    PubMed Central

    Lillywhite, Harvey B.; Sheehy, Coleman M.; Brischoux, François; Grech, Alana

    2014-01-01

    Secondarily marine vertebrates are thought to live independently of fresh water. Here, we demonstrate a paradigm shift for the widely distributed pelagic sea snake, Hydrophis (Pelamis) platurus, which dehydrates at sea and spends a significant part of its life in a dehydrated state corresponding to seasonal drought. Snakes that are captured following prolonged periods without rainfall have lower body water content, lower body condition and increased tendencies to drink fresh water than do snakes that are captured following seasonal periods of high rainfall. These animals do not drink seawater and must rehydrate by drinking from a freshwater lens that forms on the ocean surface during heavy precipitation. The new data based on field studies indicate unequivocally that this marine vertebrate dehydrates at sea where individuals may live in a dehydrated state for possibly six to seven months at a time. This information provides new insights for understanding water requirements of sea snakes, reasons for recent declines and extinctions of sea snakes and more accurate prediction for how changing patterns of precipitation might affect these and other secondarily marine vertebrates living in tropical oceans. PMID:24648228

  14. Hawksbill Sea Turtle (Eretmochelys imbricata)

    USGS Multimedia Gallery

    Hawksbill Sea Turtles (Eretmochelys imbricata), such as the one hiding here under a boulder, and Green Sea Turtles (Chelonia mydas) are occasionally seen in Hurricane Hole. Hawksbills feed mostly on sponges while Greens eat mostly sea grasses....

  15. Tracking Sea Otters

    USGS Multimedia Gallery

    USGS wildlife biologist Alisha Kage holds out a VHF receiver, hoping to hear the tell-tale beep that helps her locate sea otters that are part of study to monitor and learn more about the species. USGS scientists study sea otters in efforts to help the threatened species continue to recover from nea...

  16. Spotting Sea Otters

    USGS Multimedia Gallery

    USGS wildlife biologist Alisha Kage looks through a telescope to help her locate and identify tagged sea otters, then records the otter's location for a study aimed at learning more about the species. USGS scientists study sea otters in efforts to help the threatened species continue to recover from...

  17. Red sea drillings.

    PubMed

    Ross, D A; Whitmarsh, R B; Ali, S A; Boudreaux, J E; Coleman, R; Fleisher, R L; Girdler, R; Manheim, F; Matter, A; Nigrini, C; Stoffers, P; Supko, P R

    1973-01-26

    Recent drilling in the Red Sea has shown that much of the basin is underlain by evaporites of a similar age to that of evaporites found in the Mediterranean Sea. These evaporites and their structural positions indicate that other brine areas are present-and, indeed, several others have been discovered. PMID:17843766

  18. White Sea - Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    At bottom center of this true-color Moderate Resolution Imaging Spectroradiometer (MODIS) image from April 13, 2001, the White Sea in western Russia is becoming free of ice in its southern extent. Meanwhile, the blue-green waters along the coast of the peninsula jutting out into the Barents Sea to the northeast could be due to a phytoplankton bloom.

  19. Getting Your Sea Legs

    PubMed Central

    Stoffregen, Thomas A.; Chen, Fu-Chen; Varlet, Manuel; Alcantara, Cristina; Bardy, Benoît G.

    2013-01-01

    Sea travel mandates changes in the control of the body. The process by which we adapt bodily control to life at sea is known as getting one's sea legs. We conducted the first experimental study of bodily control as maritime novices adapted to motion of a ship at sea. We evaluated postural activity (stance width, stance angle, and the kinematics of body sway) before and during a sea voyage. In addition, we evaluated the role of the visible horizon in the control of body sway. Finally, we related data on postural activity to two subjective experiences that are associated with sea travel; seasickness, and mal de debarquement. Our results revealed rapid changes in postural activity among novices at sea. Before the beginning of the voyage, the temporal dynamics of body sway differed among participants as a function of their (subsequent) severity of seasickness. Body sway measured at sea differed among participants as a function of their (subsequent) experience of mal de debarquement. We discuss implications of these results for general theories of the perception and control of bodily orientation, for the etiology of motion sickness, and for general phenomena of perceptual-motor adaptation and learning. PMID:23840560

  20. Black Sea in Bloom

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This true-color image shows bright, turquoise-colored swirls across the surface of the Black Sea, signifying the presence of a large phytoplankton bloom. Scientists have observed similar blooms recurring annually, roughly this same time of year. The Sea of Azov, which is the smaller body of water located just north of the Black Sea in this image, also shows a high level of biological activity currently ongoing. The brownish pixels in the Azov are probably sediments carried in from high waters upstream. This scene was acquired by the Sea-viewing Wide Field-of-view Sensor (SeaWiFS), flying aboard the OrbView-2 satellite, on May 4, 2002. According to the Black Sea Environment Programme's Marine Hydrophysical Institute, the Black Sea is 'one of the marine areas of the world most damaged by human activities.' The coastal zone around these Eastern European inland water bodies is densely populated-supporting a permanent population of roughly 16 million people and another 4 million tourists each year. Six countries border with the Black Sea, including Ukraine to the north, Russia and Georgia to the east, Turkey to the south, and Bulgaria and Romania to the west. Because it is isolated from the world's oceans, and because there is an extensive drainage network of rivers that empty into it, the Black Sea has a unique and delicate water balance which is very important for supporting its marine ecosystem. Of particular concern to scientists is the salinity, water level, and nutrient levels of the Black Sea's waters, all of which are, unfortunately, being impacted by human activities. Within the last three decades the combination of increased nutrient loads from human sources together with pollution and over-harvesting of fisheries has resulted in a sharp decline in water quality. Scientists from each of the Black Sea's bordering nations are currently working together to study the issues and formulate a joint, international strategy for saving this unique marine ecosystem. Working with a spirit of placing more emphasis on joint ownership of the Black Sea's resources, and less emphasis on blame, it is hoped that the cooperating countries can strike an effective balance between both enjoying and preserving the Black Sea. Image courtesy the SeaWiFS Project, NASA GSFC, and ORBIMAGE

  1. Epibenthic assemblages in the Celtic Sea and associated with the Jones Bank

    NASA Astrophysics Data System (ADS)

    Ellis, J. R.; Martinez, I.; Burt, G. J.; Scott, B. E.

    2013-10-01

    The epibenthic assemblages in the Celtic Sea are described from the catches from 2 m beam trawl surveys undertaken from 2000 to 2009. During this period 154 samples were collected. The most ubiquitous species in the study area were the natantid shrimps Processa spp. and Crangon allmanni, the hermit crabs Pagurus prideaux and Anapagurus laevis, sand star Astropecten irregularis and spotted dragonet Callionymus maculatus. Multivariate community analyses indicated that catches (numbers per tow) were distributed across six assemblages, two of which were predominant in the study area. Most catches were attributed to either a 'shelf edge assemblage', which was widespread in deeper waters (114-423 m water depth) or an 'outer shelf assemblage' that occurred across much of the Celtic Sea north of 49°N in waters 49-175 m deep. The dominant species along the edge of the continental shelf were the hormathid anemome Actinauge richardi, sea spider Pycnogonum littorale (which associated with A. richardi), Devonshire cup coral Caryophyllia smithii and the swimming crab Macropipus tuberculatus. The dominant species in the outer shelf assemblage included P. prideaux, C. allmanni, A. laevis and common starfish Asterias rubens. Stations closer to shore were relatively distinct and catches in this 'inner shelf assemblage' were composed primarily of an inshore fauna (e.g. Ophiura ophiura, C. allmanni and Liocarcinus holsatus). Stations in the southern part of the survey grid were also relatively distinct ('southern Celtic Sea assemblage'), and several large echinoderms (Porania pulvillus, Stichastrella rosea and Anseropoda placenta) dominated at these sites. Three of the deepest stations were also relatively distinct, as were a group of stations in the muddy habitat of the Celtic Deep and comparable grounds elsewhere in the region, where Nucula sulcata and Alpheus glaber were characteristic. Catches on the shallower parts of the Jones Bank (and on another bank in the region) were dominated by the anemone Paraphellia expansa, with off-bank sites comprising a greater number of species. In contrast to beam trawl sampling, baited camera observations on the Jones Bank showed a greater richness of species on the shallower part of the bank, and provided information on the nocturnal feeding behaviour of scavenging isopods.

  2. Offshore dispersion of ephyrae and medusae of Aurelia aurita s.l. (Cnidaria: Scyphozoa) from port enclosures: Physical and biological factors

    NASA Astrophysics Data System (ADS)

    Makabe, Ryosuke; Takeoka, Hidetaka; Uye, Shin-ichi

    2015-12-01

    Recurrent outbreaks of the common jellyfish Aurelia aurita s.l. have been increasingly significant, particularly in human perturbed coastal waters, where numerous artificial constructions increase suitable habitat for polyp populations. We examined the spatiotemporal dispersion process in 6 ports of ephyrae of A. aurita after release from strobilating polyps, to offshore waters of northern Harima Nada (eutrophic eastern Inland Sea of Japan) from January to May 2010. Almost exclusive occurrence of the ephyra stage in the ports demonstrated that their seeding polyps reside in the port enclosures, and liberated ephyrae are rapidly exported offshore by tidal water exchange. Post-ephyra stages occurred primarily outside the ports, and their age increased gradually offshore, ca. up to 9 km off the ports, and the pattern of age increase could be simulated by a simple diffusion model. However, there was an abrupt decline in A. aurita density beyond ca. 3 km off the shore, where jellyfish-eating Chrysaora pacifica medusae were prevalent. We conclude that physical forces are primarily responsible for offshore dispersion of A. aurita, and a biological factor, i.e. predation by C. pacifica, jointly affects the distribution pattern of A. aurita.

  3. Simulation and observations of annual density banding in skeletons of Montastraea (Cnidaria: Scleractinia) growing under thermal stress associated with ocean warming

    USGS Publications Warehouse

    Worum, F.P.; Carricart-Ganivet, J. P.; Benson, L.; Golicher, D.

    2007-01-01

    We present a model of annual density banding in skeletons of Montastraea coral species growing under thermal stress associated with an ocean-warming scenario. The model predicts that at sea-surface temperatures (SSTs) <29??C, high-density bands (HDBs) are formed during the warmest months of the year. As temperature rises and oscillates around the optimal calcification temperature, an annual doublet in the HDB (dHDB) occurs that consists of two narrow HDBs. The presence of such dHDBs in skeletons of Montastraea species is a clear indication of thermal stress. When all monthly SSTs exceed the optimal calcification temperature, HDBs form during the coldest, not the warmest, months of the year. In addition, a decline in mean-annual calcification rate also occurs during this period of elevated SST. A comparison of our model results with annual density patterns observed in skeletons of M. faveolata and M. franksi, collected from several localities in the Mexican Caribbean, indicates that elevated SSTs are already resulting in the presence of dHDBs as a first sign of thermal stress, which occurs even without coral bleaching. ?? 2007, by the American Society of Limnology and Oceanography, Inc.

  4. Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

    NASA Astrophysics Data System (ADS)

    Schuett, Christian; Doepke, Hilke

    2010-09-01

    Endobiotic bacteria colonize the tentacles of cnidaria. This paper provides first insight into the bacterial spectrum and its potential of pathogenic activities inside four cnidarian species. Sample material originating from Scottish waters comprises the jellyfish species Cyanea capillata and C. lamarckii, hydrozoa Tubularia indivisa and sea anemone Sagartia elegans. Mixed cultures of endobiotic bacteria, pure cultures selected on basis of haemolysis, but also lyophilized samples were prepared from tentacles and used for DGGE-profiling with subsequent phylogenetic analysis of 16S rDNA fragments. Bacteria were detected in each of the cnidarian species tested. Twenty-one bacterial species including four groups of closely related organisms were found in culture material. The species within these groups could not be differentiated from each other (one group of Pseudoalteromonas spp., two groups of Shewanella spp., one group of Vibrio spp.). Each of the hosts exhibits a specific endobacterial spectrum. Solely Cyanea lamarckii harboured Moritella viscosa. Only in Cyanea capillata, members of the Shewanella group #2 and the species Pseudoalteromonas arctica, Shewanella violacea, Sulfitobacter pontiacus and Arcobacter butzleri were detected. Hydrozoa Tubularia indivisa provided an amazingly wide spectrum of nine bacterial species. Exclusively, in the sea anemone Sagartia elegans, the bacterial species P. aliena was found. Overall eleven bacterial species detected were described recently as novel species. Four 16S rDNA fragments generated from lyophilized material displayed extremely low relationship to their next neighbours. These organisms are regarded as members of the endobiotic “terra incognita”. Since the origin of cnidarian toxins is unclear, the possible pathogenic activity of endobiotic bacteria has to be taken into account. Literature data show that their next neighbours display an interesting diversity of haemolytic, septicaemic and necrotic actions including the production of cytotoxins, tetrodotoxin and R-toxin. Findings of haemolysis tests support the literature data. The potential producers are Endozoicimonas elysicola, Moritella viscosa, Photobacterium profundum, P. aliena, P. tetraodonis, Shewanella waksmanii, Vibrio splendidus, V. aestuarius, Arcobacter butzleri.

  5. Global sea level rise

    SciTech Connect

    Douglas, B.C. )

    1991-04-15

    Published values for the long-term, global mean sea level rise determined from tide gauge records exhibit considerable scatter, from about 1 mm to 3 mm/yr. This disparity is not attributable to instrument error; long-term trends computed at adjacent sites often agree to within a few tenths of a millimeter per year. Instead, the differing estimates of global sea level rise appear to be in large part due to authors' using data from gauges located at convergent tectonic plate boundaries, where changes of land elevation give fictitious sea level trends. In addition, virtually all gauges undergo subsidence or uplift due to postglacial rebound (PGR) from the last deglaciation at a rate comparable to or greater than the secular rise of sea level. Modeling PGR by the ICE-3G model of Tushingham and Peltier (1991) and avoiding tide gauge records in areas of converging tectonic plates produces a highly consistent set of long sea level records. The value for mean sea level rise obtained from a global set of 21 such stations in nine oceanic regions with an average record length of 76 years during the period 1880-1980 is 1.8 mm/yr {plus minus} 0.1. This result provides confidence that carefully selected long tide gauge records measure the same underlying trend of sea level and that many old tide gauge records are of very high quality.

  6. Dead sea water intoxication.

    PubMed

    Levy-Khademi, Floris; Brooks, Rebecca; Maayan, Channa; Tenenbaum, Ariel; Wexler, Isaiah D

    2012-08-01

    Near drowning in the Dead Sea is associated with both respiratory manifestations and severe electrolyte abnormalities. It is often difficult to distinguish between the contributions of sea water aspiration or ingestion to clinical manifestations. We present a unique case of accidental ingestion of a large amount of Dead Sea water through a gastrostomy tube in which a patient with familial dysautonomia presented with severe electrolyte disturbances. Forced diuresis with large amounts of intravenous fluids resulted in clinical and biochemical improvement. Full recovery was achieved after 2 days of treatment. PMID:22863826

  7. Sea level variation

    NASA Technical Reports Server (NTRS)

    Douglas, Bruce C.

    1992-01-01

    Published values for the long-term, global mean sea level rise determined from tide gauge records range from about one to three mm per year. The scatter of the estimates appears to arise largely from the use of data from gauges located at convergent tectonic plate boundaries where changes of land elevation give fictitious sea level trends, and the effects of large interdecadal and longer sea level variations on short (less than 50+ years) or sappy records. In addition, virtually all gauges undergo subsidence or uplift due to isostatic rebound from the last deglaciation at a rate comparable to or greater than the secular rise of sea level. Modeling rebound by the ICE-3G model of Tushingham and Peltier (1990) and avoiding tide gauge records in areas of converging tectonic plates produces a highly consistent set of long sea level records. A global set of 21 such stations in nine oceanic regions with an average record length of 76 years during the period 1880-1980 yields the global sea level rise value 1.8 mm/year +/- 0.1. Greenhouse warming scenarios commonly forecast an additional acceleration of global sea level in the next 5 or 6+ decades in the range 0.1-0.2 mm/yr2. Because of the large power at low frequencies in the sea level spectrum, very long tide gauge records (75 years minimum) have been examined for past apparent sea level acceleration. For the 80-year period 1905-1985, 23 essentially complete tide gauge records in 10 geographic groups are available for analysis. These yielded the apparent global acceleration -0.011 (+/- 0.012) mm/yr2. A larger, less uniform set of 37 records in the same 10 groups with 92 years average length covering the 141 years from 1850-1991 gave 0.001 (+/- 0.008) mm/yr2. Thus there is no evidence for an apparent acceleration in the past 100+ years that is significant either statistically, or in comparison to values associated with global warming. Unfortunately, the large interdecadal fluctuations of sea level severely affect estimates of global sea level acceleration for time spans of less than about 50 years. This means that tide gauges alone cannot serve as a reliable leading indicator of climate change in less than many decades. This time required can be significantly reduced if the interdecadal fluctuations of sea level can be understood in terms of their forcing mechanisms, and then removed from the tide gauge records.

  8. Sea Perch Project

    NASA Technical Reports Server (NTRS)

    2010-01-01

    David Lalejini, an employee of the Naval Research Laboratory at NASA's John C. Stennis Space Center, helps a pair of teachers deploy a remotely-operated underwater Sea Perch robot during workshop activities Dec. 11. The Stennis Education Office teamed with Naval Research Laboratory counterparts to conduct a two-day workshop Dec. 10-11 for Louisiana and Mississippi teachers. During the no-cost workshop, teachers learned to build and operate Sea Perch robots. The teachers now can take the Sea Perch Program back to students.

  9. Jellyfish as Prey: Frequency of Predation and Selective Foraging of Boops boops (Vertebrata, Actinopterygii) on the Mauve Stinger Pelagia noctiluca (Cnidaria, Scyphozoa)

    PubMed Central

    Fuentes, Veronica L.; Boero, Ferdinando; Guglielmo, Letterio; Purcell, Jennifer E.; Piraino, Stefano

    2014-01-01

    In recent years, jellyfish blooms have attracted considerable scientific interest for their potential impacts on human activities and ecosystem functioning, with much attention paid to jellyfish as predators and to gelatinous biomass as a carbon sink. Other than qualitative data and observations, few studies have quantified direct predation of fish on jellyfish to clarify whether they may represent a seasonally abundant food source. Here we estimate predation frequency by the commercially valuable Mediterranean bogue, Boops boops on the mauve stinger jellyfish, Pelagia noctiluca, in the Strait of Messina (NE Sicily). A total of 1054 jellyfish were sampled throughout one year to quantify predation by B. boops from bite marks on partially eaten jellyfish and energy density of the jellyfish. Predation by B. boops in summer was almost twice that in winter, and they selectively fed according to medusa gender and body part. Calorimetric analysis and biochemical composition showed that female jellyfish gonads had significantly higher energy content than male gonads due to more lipids and that gonads had six-fold higher energy content than the somatic tissues due to higher lipid and protein concentrations. Energetically, jellyfish gonads represent a highly rewarding food source, largely available to B. boops throughout spring and summer. During the remainder of the year, when gonads were not very evident, fish predation switched towards less-selective foraging on the somatic gelatinous biomass. P. noctiluca, the most abundant jellyfish species in the Mediterranean Sea and a key planktonic predator, may represent not only a nuisance for human leisure activities and a source of mortality for fish eggs and larvae, but also an important resource for fish species of commercial value, such as B. boops. PMID:24727977

  10. Timoides agassizii Bigelow, 1904, little-known hydromedusa (Cnidaria), appears briefly in large numbers off Oman, March 2011, with additional notes about species of the genus Timoides.

    PubMed

    Purushothaman, Jasmine; Kharusi, Lubna Al; Mills, Claudia E; Ghielani, Hamed; Marzouki, Mohammad Al

    2013-01-01

    A bloom of the hydromedusan jellyfish, Timoides agassizii, occurred in February 2011 off the coast of Sohar, Al Batinah, Sultanate of Oman, in the Gulf of Oman. This species was first observed in 1902 in great numbers off Haddummati Atoll in the Maldive Islands in the Indian Ocean and has rarely been seen since. The species appeared briefly in large numbers off Oman in 2011 and subsequent observation of our 2009 samples of zooplankton from Sohar revealed that it was also present in low numbers (two collected) in one sample in 2009; these are the first records in the Indian Ocean north of the Maldives. Medusae collected off Oman were almost identical to those recorded previously from the Maldive Islands, Papua New Guinea, the Marshall Islands, Guam, the South China Sea, and Okinawa. T. agassizii is a species that likely lives for several months. It was present in our plankton samples together with large numbers of the oceanic siphonophore Physalia physalis only during a single month's samples, suggesting that the temporary bloom off Oman was likely due to the arrival of mature, open ocean medusae into nearshore waters. We see no evidence that T. agassizii has established a new population along Oman, since if so, it would likely have been present in more than one sample period. We are unable to deduce further details of the life cycle of this species from blooms of many mature individuals nearshore, about a century apart. Examination of a single damaged T. agassizii medusa from Guam, calls into question the existence of its congener, T. latistyla, known only from a single specimen. PMID:25113482

  11. Sea Ice Ecosystems

    NASA Astrophysics Data System (ADS)

    Arrigo, Kevin R.

    2014-01-01

    Polar sea ice is one of the largest ecosystems on Earth. The liquid brine fraction of the ice matrix is home to a diverse array of organisms, ranging from tiny archaea to larger fish and invertebrates. These organisms can tolerate high brine salinity and low temperature but do best when conditions are milder. Thriving ice algal communities, generally dominated by diatoms, live at the ice/water interface and in recently flooded surface and interior layers, especially during spring, when temperatures begin to rise. Although protists dominate the sea ice biomass, heterotrophic bacteria are also abundant. The sea ice ecosystem provides food for a host of animals, with crustaceans being the most conspicuous. Uneaten organic matter from the ice sinks through the water column and feeds benthic ecosystems. As sea ice extent declines, ice algae likely contribute a shrinking fraction of the total amount of organic matter produced in polar waters.

  12. Sea level change

    SciTech Connect

    Meier, M.F.

    1996-12-31

    The IPCC (Intergovernmental Panel on Climate Change) 1995 Scientific Assessment, Chapter 7. Sea Level Change, presents a modest revision of the similar chapter in the 1990 Assessment. Principal conclusions on observed sea-level change and the principal terms in the sea-level equation (ocean thermal expansion, glaciers, ice sheets, and land hydrology), including our knowledge of the present-day (defined as the 20th Century) components of sea-level rise, and projections of these for the future, are presented here. Some of the interesting glaciological problems which are involved in these studies are discussed in more detail. The emphasis here is on trends over decades to a century, not on shorter variations nor on those of the geologic past. Unfortunately, some of the IPCC projections had not been agreed at the time of writing of this paper, and these projections will not be given here. 15 refs., 2 figs.

  13. North Sea rift system

    NASA Astrophysics Data System (ADS)

    Ziegler, P. A.

    1992-07-01

    The Mesozoic North Sea rift forms an integral part of the Arctic-North Atlantic rift system. Rifting activity in the North Sea commenced during the earliest Triassic. During the mid-Jurassic the central North Sea underwent a temporary doming stage. Crustal extension peaked during the Late Jurassic-earliest Cretaceous, abated subsequently and terminated altogether during the Paleocene. The saucer-shaped Cenozoic post-rift basin straddles the axis of the Viking and Central grabens. This speaks for the applicability of a depth dependent pure shear model rather than a simple shear model. Geophysical data indicate an important discrepancy between upper and lower crustal attenuation across the North Sea rift, suggesting that during rifting processes the Mono-discontinuity was seriously destabilized. This requires a basic modification of currently favoured crustal stretching models.

  14. Dead Sea Scrolls

    NASA Technical Reports Server (NTRS)

    1994-01-01

    A consortium of researchers from Jet Propulsion Laboratory and three other organizations used charged coupled devices (CCDs) and other imaging enhancement technology to decipher previously unreadable portions of the Dead Sea Scrolls. The technique has potentially important implications for archeology.

  15. Alaska: Beaufort Sea

    Atmospheric Science Data Center

    2014-05-15

    ... satellite remote sensing instruments; the synthetic aperture radar (SAR) on board the RADARSAT satellite and the Multi-angle Imaging ... by how the surface and subsurface roughness influence radar backscatter. In the SAR image, white lines delineate different sea ice ...

  16. Record Sea Ice Minimum

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Arctic sea ice reached a record low in September 2007, below the previous record set in 2005 and substantially below the long-term average. This image shows the Arctic as observed by the Advanced Microwave Scanning Radiometer for EOS (AMSR-E) aboard NASA's Aqua satellite on September 16, 2007. In this image, blue indicates open water, white indicates high sea ice concentration, and turquoise indicates loosely packed sea ice. The black circle at the North Pole results from an absence of data as the satellite does not make observations that far north. Three contour lines appear on this image. The red line is the 2007 minimum, as of September 15, about the same time the record low was reached, and it almost exactly fits the sea ice observed by AMSR-E. The green line indicates the 2005 minimum, the previous record low. The yellow line indicates the median minimum from 1979 to 2000.

  17. Sensing the sea bed

    NASA Astrophysics Data System (ADS)

    2009-07-01

    William Wilcock and a team of scientists and engineers drilled holes in the sea floor, and inadvertently provided a breeding ground for octopuses, in their attempt to understand deep-ocean hydrothermal venting.

  18. Teacher at Sea.

    ERIC Educational Resources Information Center

    Beighley, Karl

    1998-01-01

    Outlines the experiences of a teacher in the National Oceanic and Atmospheric Administration's (NOAA) Teacher At Sea Program in which teachers are placed on NOAA vessels to work with professional scientists doing critical, real world research. (DDR)

  19. Black Sea Becomes Turquoise

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This true-color image shows bright, turquoise-colored swirls across the surface of the Black Sea, signifying the presence of a large phytoplankton bloom. Scientists have observed similar blooms recurring annually, roughly this same time of year. The Sea of Azov, which is the smaller body of water located just north of the Black Sea in this image, also shows a high level of color variance. The brownish pixels in the Azov are probably due to sediments carried in from high waters and snowmelt from upstream. This scene was acquired by the Moderate Resolution Imaging Spectroradiometer, flying aboard NASA's Terra satellite, on May 14, 2002. According to the Black Sea Environment Programme's Marine Hydrophysical Institute, the Black Sea is ?one of the marine areas of the world most damaged by human activities.? The coastal zone around these Eastern European inland water bodies is densely populated'supporting a permanent population of roughly 16 million people and another 4 million tourists each year. Six countries border with the Black Sea, including Ukraine to the north, Russia and Georgia to the east, Turkey to the south, and Bulgaria and Romania to the west. Because it is isolated from the world's oceans, and because there is an extensive drainage network of rivers that empty into it, the Black Sea has a unique and delicate water balance which is very important for supporting its marine ecosystem. Of particular concern to scientists is the salinity, water level, and nutrient levels of the Black Sea's waters, all of which are, unfortunately, being impacted by human activities. Within the last three decades the combination of increased nutrient loads from human sources together with pollution and over-harvesting of fisheries has resulted in a sharp decline in water quality. Scientists from each of the Black Sea's bordering nations are currently working together to study the issues and formulate a joint, international strategy for saving this unique marine ecosystem. Working with a spirit of placing more emphasis on joint ownership of the Black Sea's resources, and less emphasis on blame, it is hoped that the cooperating countries can strike an effective balance between both enjoying and preserving the Black Sea.

  20. South China Sea.

    PubMed

    Morton, B; Blackmore, G

    2001-12-01

    The South China Sea is poorly understood in terms of its marine biota, ecology and the human impacts upon it. What is known is most often contained in reports and workshop and conference documents that are not available to the wider scientific community. The South China Sea has an area of some 3.3 million km2 and depths range from the shallowest coastal fringe to 5377 m in the Manila Trench. It is also studded with numerous islets, atolls and reefs many of which are just awash at low tide. It is largely confined within the Tropic of Cancer and, therefore, experiences a monsoonal climate being influenced by the Southwest Monsoon in summer and the Northeast Monsoon in winter. The South China Sea is a marginal sea and, therefore, largely surrounded by land. Countries that have a major influence on and claims to the sea include China, Malaysia, the Philippines and Vietnam, although Thailand, Indonesia and Taiwan have some too. The coastal fringes of the South China Sea are home to about 270 million people that have had some of the fastest developing and most vibrant economies on the globe. Consequently, anthropogenic impacts, such as over-exploitation of resources and pollution, are anticipated to be huge although, in reality, relatively little is known about them. The Indo-West Pacific biogeographic province, at the centre of which the South China Sea lies, is probably the world's most diverse shallow-water marine area. Of three major nearshore habitat types, i.e., coral reefs, mangroves and seagrasses, 45 mangrove species out of a global total of 51, most of the currently recognised 70 coral genera and 20 of 50 known seagrass species have been recorded from the South China Sea. The island groups of the South China Sea are all disputed and sovereignty is claimed over them by a number of countries. Conflicts have in recent decades arisen over them because of perceived national rights. It is perhaps because of this that so little research has been undertaken on the South China Sea. What data are available, however, and if Hong Kong is used, as it is herein, as an indicator of what the perturbations of other regional cities upon the South China Sea are like, then it is impacted grossly and an ecological disaster has probably already, but unknowingly, happened. PMID:11827109

  1. Microdistribution of Faunal Assemblages at Deep-Sea Hydrothermal Vents in the Southern Ocean

    PubMed Central

    Marsh, Leigh; Copley, Jonathan T.; Huvenne, Veerle A. I.; Linse, Katrin; Reid, William D. K.; Rogers, Alex D.; Sweeting, Christopher J.; Tyler, Paul A.

    2012-01-01

    Chemosynthetic primary production by microbes supports abundant faunal assemblages at deep-sea hydrothermal vents, with zonation of invertebrate species typically occurring along physico-chemical gradients. Recently discovered vent fields on the East Scotia Ridge (ESR) in the Southern Ocean represent a new province of vent biogeography, but the spatial dynamics of their distinct fauna have yet to be elucidated. This study determines patterns of faunal zonation, species associations, and relationships between faunal microdistribution and hydrothermal activity in a vent field at a depth of 2,400 m on the ESR. Remotely operated vehicle (ROV) dives obtained high-definition imagery of three chimney structures with varying levels of hydrothermal activity, and a mosaic image of >250 m2 of seafloor co-registered with temperature measurements. Analysis of faunal microdistribution within the mosaiced seafloor reveals a consistent pattern of faunal zonation with increasing distance from vent sources and peak temperatures. Assemblages closest to vent sources are visibly dominated by a new species of anomuran crab, Kiwa n. sp. (abundance >700 individuals m−2), followed by a peltospiroid gastropod (>1,500 individuals m−2), eolepadid barnacle (>1,500 individuals m−2), and carnivorous actinostolid anemone (>30 individuals m−2). Peripheral fauna are not dominated by a single taxon, but include predatory and scavenger taxa such as stichasterid seastars, pycnogonids and octopus. Variation in faunal microdistribution on chimneys with differing levels of activity suggests a possible successional sequence for vent fauna in this new biogeographic province. An increase in δ34S values of primary consumers with distance from vent sources, and variation in their δ13C values also indicate possible zonation of nutritional modes of the vent fauna. By using ROV videography to obtain a high-resolution representation of a vent environment over a greater extent than previous studies, these results provide a baseline for determining temporal change and investigations of processes structuring faunal assemblages at Southern Ocean vents. PMID:23144754

  2. Microdistribution of faunal assemblages at deep-sea hydrothermal vents in the Southern Ocean.

    PubMed

    Marsh, Leigh; Copley, Jonathan T; Huvenne, Veerle A I; Linse, Katrin; Reid, William D K; Rogers, Alex D; Sweeting, Christopher J; Tyler, Paul A

    2012-01-01

    Chemosynthetic primary production by microbes supports abundant faunal assemblages at deep-sea hydrothermal vents, with zonation of invertebrate species typically occurring along physico-chemical gradients. Recently discovered vent fields on the East Scotia Ridge (ESR) in the Southern Ocean represent a new province of vent biogeography, but the spatial dynamics of their distinct fauna have yet to be elucidated. This study determines patterns of faunal zonation, species associations, and relationships between faunal microdistribution and hydrothermal activity in a vent field at a depth of 2,400 m on the ESR. Remotely operated vehicle (ROV) dives obtained high-definition imagery of three chimney structures with varying levels of hydrothermal activity, and a mosaic image of >250 m(2) of seafloor co-registered with temperature measurements. Analysis of faunal microdistribution within the mosaiced seafloor reveals a consistent pattern of faunal zonation with increasing distance from vent sources and peak temperatures. Assemblages closest to vent sources are visibly dominated by a new species of anomuran crab, Kiwa n. sp. (abundance >700 individuals m(-2)), followed by a peltospiroid gastropod (>1,500 individuals m(-2)), eolepadid barnacle (>1,500 individuals m(-2)), and carnivorous actinostolid anemone (>30 individuals m(-2)). Peripheral fauna are not dominated by a single taxon, but include predatory and scavenger taxa such as stichasterid seastars, pycnogonids and octopus. Variation in faunal microdistribution on chimneys with differing levels of activity suggests a possible successional sequence for vent fauna in this new biogeographic province. An increase in ?(34)S values of primary consumers with distance from vent sources, and variation in their ?(13)C values also indicate possible zonation of nutritional modes of the vent fauna. By using ROV videography to obtain a high-resolution representation of a vent environment over a greater extent than previous studies, these results provide a baseline for determining temporal change and investigations of processes structuring faunal assemblages at Southern Ocean vents. PMID:23144754

  3. Late Holocene radiocarbon and aspartic acid racemization dating of deep-sea octocorals

    NASA Astrophysics Data System (ADS)

    Sherwood, Owen A.; Scott, David B.; Risk, Michael J.

    2006-06-01

    Primnoa resedaeformis is a deep-sea gorgonian coral with a two-part skeleton of calcite and gorgonin (a fibrillar protein), potentially containing long-term records of valuable paleo-environmental information. For various reasons, both radiocarbon and U/Th dating of these corals is problematic over the last few centuries. This paper explores aspartic acid racemization dating of the gorgonin fraction in modern and fossil specimens collected from the NW Atlantic Ocean. Radiocarbon dating of the fossil specimen indicates a lifespan of 700 ± 100 years, the longest yet documented for any octocoral. Gorgonin amino acid compositions were identical in the fossil and modern specimens, indicating resistance to organic diagenesis. Similar to bone collagen, the fibrillar protein of gorgonin may impose conformational constraints on the racemization of Asp at low temperatures. The rate of racemization of aspartic acid ( D/ L-Asp) was similar to previously published results from an 1800 year old anemone ( Gerardia). The age equation was: age (years BP 2000 AD) = [( D/ L - 0.020 (±.002))/.0011 (±.0001)] 2 ( r2 = 0.97, p < .001). The error in an age estimate calculated by D/ L-Asp was marginally better than that for 14C dating over the most recent 50-200 years, although the dating error may be improved by inclusion of more samples over a broader time range. These results suggest that D/ L-Asp dating may be useful in augmenting 14C dating in cases where 14C calibrations yield two or more intercept ages, or in screening samples for further 14C or U/Th dating.

  4. Contemporary sea level rise.

    PubMed

    Cazenave, Anny; Llovel, William

    2010-01-01

    Measuring sea level change and understanding its causes has considerably improved in the recent years, essentially because new in situ and remote sensing observations have become available. Here we report on most recent results on contemporary sea level rise. We first present sea level observations from tide gauges over the twentieth century and from satellite altimetry since the early 1990s. We next discuss the most recent progress made in quantifying the processes causing sea level change on timescales ranging from years to decades, i.e., thermal expansion of the oceans, land ice mass loss, and land water-storage change. We show that for the 1993-2007 time span, the sum of climate-related contributions (2.85 +/- 0.35 mm year(-1)) is only slightly less than altimetry-based sea level rise (3.3 +/- 0.4 mm year(-1)): approximately 30% of the observed rate of rise is due to ocean thermal expansion and approximately 55% results from land ice melt. Recent acceleration in glacier melting and ice mass loss from the ice sheets increases the latter contribution up to 80% for the past five years. We also review the main causes of regional variability in sea level trends: The dominant contribution results from nonuniform changes in ocean thermal expansion. PMID:21141661

  5. Early and late response of Nematostella vectensis transcriptome to heavy metals.

    PubMed

    Elran, Ron; Raam, Maayan; Kraus, Roey; Brekhman, Vera; Sher, Noa; Plaschkes, Inbar; Chalifa-Caspi, Vered; Lotan, Tamar

    2014-10-01

    Environmental contamination from heavy metals poses a global concern for the marine environment, as heavy metals are passed up the food chain and persist in the environment long after the pollution source is contained. Cnidarians play an important role in shaping marine ecosystems, but environmental pollution profoundly affects their vitality. Among the cnidarians, the sea anemone Nematostella vectensis is an advantageous model for addressing questions in molecular ecology and toxicology as it tolerates extreme environments and its genome has been published. Here, we employed a transcriptome-wide RNA-Seq approach to analyse N. vectensis molecular defence mechanisms against four heavy metals: Hg, Cu, Cd and Zn. Altogether, more than 4800 transcripts showed significant changes in gene expression. Hg had the greatest impact on up-regulating transcripts, followed by Cu, Zn and Cd. We identified, for the first time in Cnidaria, co-up-regulation of immediate-early transcription factors such as Egr1, AP1 and NF-?B. Time-course analysis of these genes revealed their early expression as rapidly as one hour after exposure to heavy metals, suggesting that they may complement or substitute for the roles of the metal-mediating Mtf1 transcription factor. We further characterized the regulation of a large array of stress-response gene families, including Hsp, ABC, CYP members and phytochelatin synthase, that may regulate synthesis of the metal-binding phytochelatins instead of the metallothioneins that are absent from Cnidaria genome. This study provides mechanistic insight into heavy metal toxicity in N. vectensis and sheds light on ancestral stress adaptations. PMID:25145541

  6. Annual sea ice. An air-sea gas exchange moderator

    SciTech Connect

    Gosink, T.A.; Kelley, J.J.

    1982-01-01

    Arctic annual sea ice, particularly when it is relatively warm (> -15/sup 0/C) permits significant gas exchange between the sea and air throughout the entire year. Sea ice, particularly annual sea ice, differs from freshwater ice with respect to its permeability to gases. The presence of brine allows for significant air-sea-ice exchange of CO/sub 2/ throughout the winter, which may significantly affect the global carbon dioxide balance. Other trace gases are also noted to be enriched in sea ice, but less is known about their importance to air-sea-interactions at this time. Both physical and biological factors cause and modify evolution of gases from the surface of sea ice. Quantitative and qualitative descriptions of the nature and physical behavior of sea ice with respect to brine and gases are discussed.

  7. The White Sea, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Editor's Note: The caption below, published on May 10, 2001, is incorrect. According to Masha Vorontsova, director of the International Fund for Animal Welfare in Moscow, the situation with the seal pups in the White Sea is normal. There is no disaster and there never was. For more details, refer to the article entitled 'No Danger' on the New Scientist home page. The Earth Observatory regrets the earlier errant report. Original Caption According to the Russian Polar Research Institute for Fisheries and Oceanography, between 250,000 and 300,000 Greenland seal pups face death by starvation over the next two months due to a cruel trick by mother nature. The seals, most of them less than two months old, are trapped on ice sheets that remain locked in the White Sea, located near Archangel in Northern Russia. Typically, during the spring thaw the ice sheets break up and flow with the currents northward into the Barents Sea, the seals' spring feeding grounds. The seal pups hitch a ride on the ice floes, living on their own individual stores of fat until they arrive in the Barents Sea. Their mothers departed for the Barents Sea weeks ago. In a normal year, the seal pups' trip from the White Sea out to the Barents takes about six weeks and the seals have adapted to rely upon this mechanism of mother nature. During their yearly migration, the mother seals usually stay with their pups and feed them until their pelts turn from white to grey--a sign that the pups are mature enough to swim and feed themselves. Unfortunately, this year unusually strong northerly winds created a bottleneck of ice near the mouth of the white sea, thus blocking the flow of ice and trapping the pups. These true-color images of the White Sea were acquired by the Moderate-resolution Imaging Spectroradiometer (MODIS), flying aboard NASA's Terra spacecraft. This image, taken May 2, 2000 that there is usually much less ice in the White Sea this time of year as most of it is typically en route to the Barents Sea.

  8. The Dead Sea

    NASA Technical Reports Server (NTRS)

    2006-01-01

    The Dead Sea is the lowest point on Earth at 418 meters below sea level, and also one of the saltiest bodies of water on Earth with a salinity of about 300 parts-per-thousand (nine times greater than ocean salinity). It is located on the border between Jordan and Israel, and is fed by the Jordan River. The Dead Sea is located in the Dead Sea Rift, formed as a result of the Arabian tectonic plate moving northward away from the African Plate. The mineral content of the Dead Sea is significantly different from that of ocean water, consisting of approximately 53% magnesium chloride, 37% potassium chloride and 8% sodium chloride. In the early part of the 20th century, the Dead Sea began to attract interest from chemists who deduced that the Sea was a natural deposit of potash and bromine. From the Dead Sea brine, Israel and Jordan produce 3.8 million tons potash, 200,000 tons elemental bromine, 45,000 tons caustic soda, 25, 000 tons magnesium metal, and sodium chloride. Both countries use extensive salt evaporation pans that have essentially diked the entire southern end of the Dead Sea.

    With its 14 spectral bands from the visible to the thermal infrared wavelength region, and its high spatial resolution of 15 to 90 meters (about 50 to 300 feet), ASTER images Earth to map and monitor the changing surface of our planet.

    ASTER is one of five Earth-observing instruments launched December 18, 1999, on NASA's Terra satellite. The instrument was built by Japan's Ministry of Economy, Trade and Industry. A joint U.S./Japan science team is responsible for validation and calibration of the instrument and the data products.

    The broad spectral coverage and high spectral resolution of ASTER provides scientists in numerous disciplines with critical information for surface mapping, and monitoring of dynamic conditions and temporal change. Example applications are: monitoring glacial advances and retreats; monitoring potentially active volcanoes; identifying crop stress; determining cloud morphology and physical properties; wetlands evaluation; thermal pollution monitoring; coral reef degradation; surface temperature mapping of soils and geology; and measuring surface heat balance.

    The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission Directorate.

    Size: 18.5 by 48.1 kilometers (11.5 by 29.8 miles) Location: 31.4 degrees North latitude, 35.4 degrees East longitude Orientation: North at top Image Data: ASTER bands 3, 2, and 1 Original Data Resolution: 15 meters (49.2 feet) Dates Acquired: May 3, 2005

  9. Caribbean Sea Level Network

    NASA Astrophysics Data System (ADS)

    von Hillebrandt-Andrade, C.; Crespo Jones, H.

    2012-12-01

    Over the past 500 years almost 100 tsunamis have been observed in the Caribbean and Western Atlantic, with at least 3510 people having lost their lives to this hazard since 1842. Furthermore, with the dramatic increase in population and infrastructure along the Caribbean coasts, today, millions of coastal residents, workers and visitors are vulnerable to tsunamis. The UNESCO IOC Intergovernmental Coordination Group for Tsunamis and other Coastal Hazards for the Caribbean and Adjacent Regions (CARIBE EWS) was established in 2005 to coordinate and advance the regional tsunami warning system. The CARIBE EWS focuses on four areas/working groups: (1) Monitoring and Warning, (2) Hazard and Risk Assessment, (3) Communication and (4) Education, Preparedness and Readiness. The sea level monitoring component is under Working Group 1. Although in the current system, it's the seismic data and information that generate the initial tsunami bulletins, it is the data from deep ocean buoys (DARTS) and the coastal sea level gauges that are critical for the actual detection and forecasting of tsunamis impact. Despite multiple efforts and investments in the installation of sea level stations in the region, in 2004 there were only a handful of sea level stations operational in the region (Puerto Rico, US Virgin Islands, Bermuda, Bahamas). Over the past 5 years there has been a steady increase in the number of stations operating in the Caribbean region. As of mid 2012 there were 7 DARTS and 37 coastal gauges with additional ones being installed or funded. In order to reach the goal of 100 operational coastal sea level stations in the Caribbean, the CARIBE EWS recognizes also the importance of maintaining the current stations. For this, a trained workforce in the region for the installation, operation and data analysis and quality control is considered to be critical. Since 2008, three training courses have been offered to the sea level station operators and data analysts. Other requirements and factors have been considered for the sustainability of the stations. The sea level stations have to potentially sustain very aggressive conditions of not only tsunamis, but on a more regular basis, hurricanes. Given the requirement that the data be available in near real time, for tsunami and other coastal hazard application, robust communication systems are also essential. For the local operator, the ability to be able to visualize the data is critical and tools like the IOC Sea level Monitoring Facility and the Tide Tool program are very useful. It has also been emphasized the need for these stations to serve multiple purposes. For climate and other research applications the data need to be archived, QC'd and analyzed. Increasing the user base for the sea level data has also been seen as an important goal to gain the local buy in; local weather and meteorological offices are considered as key stakeholders but for whom applications still need to be developed. The CARIBE EWS continues to look forward to working with other IOC partners including the Global Sea Level Observing System (GLOSS) and Sub-Commission for the Caribbean and Adjacent Regions (IOCARIBE)/GOOS, as well as with local, national and global sea level station operators and agencies for the development of a sustainable sea level network.

  10. Geology of Barents Sea

    SciTech Connect

    Riis, F.; Vollset, J.

    1984-09-01

    The Barents Sea is situated on the continental shelf between Norway, the Spitsbergen Islands, and Novaya Zemlya. The main structural framework of the area was formed during the Caledonian and Hercynian orogenies, whereas the western parts were reactivated by the Kimmerian and Alpine orogenies. Because of the complex opening of the Greenland Norwegian Sea, important tertiary reactivation of Mesozoic normal faults occurred along southwest-northeast-trending systems of wrench faults. Owing to substantial erosion in the late Tertiary, the subsidence history and thermal development are more difficult to unravel in this area than in other places along the Norwegian Shelf. The erosion products were deposited in a huge sedimentary wedge extending onto the oceanic crust. The hydrocarbon discoveries in the Troms area in the southern part of the Barents Sea are encouraging for further exploration. However, the petroleum potential for large areas is not well known at this stage.

  11. Understanding Sea Level Changes

    NASA Technical Reports Server (NTRS)

    Chao, Benjamin F.

    2004-01-01

    Today more than 100 million people worldwide live on coastlines within one meter of mean sea level; any short-term or long-term sea level change relative to vertical ground motion is of great societal and economic concern. As palm-environment and historical data have clearly indicated the existence and prevalence of such changes in the past, new scientific information regarding to the nature and causes and a prediction capability are of utmost importance for the future. The 10-20 cm global sea-level rise recorded over the last century has been broadly attributed to two effects: (1) the steric effect (thermal expansion and salinity-density compensation of sea water) following global climate; (2) mass-budget changes due to a number of competing geophysical and hydrological processes in the Earth-atmosphere-hydrosphere-cryosphere system, including water exchange from polar ice sheets and mountain glaciers to the ocean, atmospheric water vapor and land hydrological variations, and anthropogenic effects such as water impoundment in artificial reservoirs and extraction of groundwater, all superimposed on the vertical motions of solid Earth due to tectonics, rebound of the mantle from past and present deglaciation, and other local ground motions. As remote-sensing tools, a number of space geodetic measurements of sea surface topography (e.g., TOPEX/Poseidon, Jason), ice mass (e.g., ICESat), time-variable gravity (e.g. GRACE), and ground motions (SLR, VLBI, GPS, InSAR, Laser altimetry, etc.) become directly relevant. Understanding sea level changes "anywhere, anytime" in a well-defined terrestrial reference frame in terms of climate change and interactions among ice masses, oceans, and the solid Earth, and being able to predict them, emerge as one of the scientific challenges in the Solid Earth Science Working Group (SESWG, 2003) conclusions.

  12. Spectacled Eiders Wintering in Northern Bering Sea

    USGS Multimedia Gallery

    Large flock of Spectacled Eiders aggregated in a sea ice lead and on sea ice in the northern Bering Sea, south of St. Lawrence Island, Alaska.  Aerial photograph from a helicopter deployed from the USCG Cutter Polar Sea....

  13. Seafloor Control on Sea Ice

    NASA Technical Reports Server (NTRS)

    Nghiem, S. V.; Clemente-Colon, P.; Rigor, I. G.; Hall, D. K.; Neumann, G.

    2011-01-01

    The seafloor has a profound role in Arctic sea ice formation and seasonal evolution. Ocean bathymetry controls the distribution and mixing of warm and cold waters, which may originate from different sources, thereby dictating the pattern of sea ice on the ocean surface. Sea ice dynamics, forced by surface winds, are also guided by seafloor features in preferential directions. Here, satellite mapping of sea ice together with buoy measurements are used to reveal the bathymetric control on sea ice growth and dynamics. Bathymetric effects on sea ice formation are clearly observed in the conformation between sea ice patterns and bathymetric characteristics in the peripheral seas. Beyond local features, bathymetric control appears over extensive ice-prone regions across the Arctic Ocean. The large-scale conformation between bathymetry and patterns of different synoptic sea ice classes, including seasonal and perennial sea ice, is identified. An implication of the bathymetric influence is that the maximum extent of the total sea ice cover is relatively stable, as observed by scatterometer data in the decade of the 2000s, while the minimum ice extent has decreased drastically. Because of the geologic control, the sea ice cover can expand only as far as it reaches the seashore, the continental shelf break, or other pronounced bathymetric features in the peripheral seas. Since the seafloor does not change significantly for decades or centuries, sea ice patterns can be recurrent around certain bathymetric features, which, once identified, may help improve short-term forecast and seasonal outlook of the sea ice cover. Moreover, the seafloor can indirectly influence cloud cover by its control on sea ice distribution, which differentially modulates the latent heat flux through ice covered and open water areas.

  14. Egyptian Sea Cave

    ERIC Educational Resources Information Center

    Journal of College Science Teaching, 2005

    2005-01-01

    This brief article describes an archaeological expedition to the Red Sea coast area of Egypt in 2004. Kathryn Bard, an associate professor of archaeology at Boston University, along with her team, discovered the well-preserved cedar timbers of an ancient Egyptian seafaring vessel near the entrance to a large man-made cave. Limestone tablets with…

  15. The Provident Sea

    NASA Astrophysics Data System (ADS)

    Cushing, David H.

    1988-09-01

    The Provident Sea describes the history of fish stock management (including whales and seals). The book traces, on the basis of the original scientific material, the history of the management of "the provident sea" up to recent times when problems of over-exploitation have had dramatic effects upon stocks. The need for management arose mainly from the increasing industrialization of capture. Hence the preindustrial fisheries are covered, in particular the old cod fishery on the Grand Bank and the herring fishery in the North Sea, as an essential background to current problems. The origins of fisheries and whaling science are described, as is the development up to 1965 of the science and institution in fisheries, whaling, and sealing. In the sixties and seventies, certain major fishing nations took a heavy harvest of fish stocks using sophisticated and efficient gathering methods. This in turn led to conflict and one consequence was the "Law of the Sea" conference set up to try and resolve these issues.

  16. S.E.A.

    ERIC Educational Resources Information Center

    Hawkins, Richard H.

    1976-01-01

    Sea Semester combines a six-week apprenticeship on a sailing ship with an intensive shore preparation component. Through Boston University, students learn marine and nautical sciences before putting some of this information to practice. Students, having completed the shore and sailing components, can enroll in more advanced shore component…

  17. S.E.A.

    ERIC Educational Resources Information Center

    Hawkins, Richard H.

    1976-01-01

    Sea Semester combines a six-week apprenticeship on a sailing ship with an intensive shore preparation component. Through Boston University, students learn marine and nautical sciences before putting some of this information to practice. Students, having completed the shore and sailing components, can enroll in more advanced shore component…

  18. Solomon's Sea and [Pi

    ERIC Educational Resources Information Center

    Simoson, Andrew J.

    2009-01-01

    This paper is a whimsical survey of the various explanations which might account for the biblical passage in I Kings 7:23 that describes a round object--a bronze basin called Solomon's Sea--as having diameter ten cubits and circumference thirty cubits. Can the biblical pi be any number other than 3? We offer seven different perspectives on this…

  19. Sea Surface Temperature

    EPA Science Inventory

    This indicator presents global mean sea surface temperature (SST) anomalies from 1880 to 2010. SST is a critical physical attribute of marine and coastal ecosystems that directly affects biological and physical process rates, water column stability, and the presence and health...

  20. Solar Sea Power

    ERIC Educational Resources Information Center

    Zener, Clarence

    1976-01-01

    In their preoccupation with highly complex new energy systems, scientists and statesmen may be overlooking the possibilities of Ocean Thermal Energy Conversion (OTEC). That is the view of a Carnegie-Mellon University physicist who is in the forefront of solar sea power investigation. (Author/BT)

  1. Harvesting the Sea.

    ERIC Educational Resources Information Center

    Markle, Sandra

    1989-01-01

    Information on commercial fishing is provided, and a board game is described which requires students to watch for ocean signs of good fishing, choose equipment to catch specific types of fish, and consider effects of weather on working at sea. A reproducible copy of the game, with instructions, is included. (IAH)

  2. Farming the Sea

    ERIC Educational Resources Information Center

    Morgan, William

    1971-01-01

    Florida has initiated a training program in an entirely new dimension--Sea Farming. Presented is a description of the vocational agriculture program designed to teach propagation, cultivation, harvesting, marketing, and conservation practices related to production of oysters, shrimp, scallops, crabs, and fin fishes. (Editor/GB)

  3. Learning from the Sea

    ERIC Educational Resources Information Center

    Acker, Susan

    1973-01-01

    Introduces a series of articles relating to learning from the sea. Marine science programs provide a rich experience for students making them aware of interrelationships and concerned about preserving marine habitats and solving environmental problems. Stresses the importance of involvement in successfully studying the ocean world. (JR)

  4. Ships to the Sea.

    ERIC Educational Resources Information Center

    Department of the Navy, Washington, DC.

    This lesson contains materials for the U.S. Navy Museum's "Ships to the Sea" program. The program is appropriate for students in grades 2-4 and was designed in accordance with local and national social studies standards. The materials introduce students to the world of ship technology and naval terminology. The lesson is presented in five…

  5. Egyptian Sea Cave

    ERIC Educational Resources Information Center

    Journal of College Science Teaching, 2005

    2005-01-01

    This brief article describes an archaeological expedition to the Red Sea coast area of Egypt in 2004. Kathryn Bard, an associate professor of archaeology at Boston University, along with her team, discovered the well-preserved cedar timbers of an ancient Egyptian seafaring vessel near the entrance to a large man-made cave. Limestone tablets with…

  6. Sea Grant's Education Mission.

    ERIC Educational Resources Information Center

    Sherman, Douglas

    1998-01-01

    Considers the status of the education agenda of the Sea Grant Program as it turns 30. Projects described include Operation Pathfinder, which aims to educate minority teachers and/or teachers of minority students. Also described are a program in which seafood processors and resellers are trained in safety and health areas, and programs to train…

  7. Virtual sea border

    NASA Astrophysics Data System (ADS)

    Ferriere, D.; Rucinski, A.; Jankowski, T.

    2007-04-01

    Establishing a Virtual Sea Border by performing a real-time, satellite-accessible Internet-based bio-metric supported threat assessment of arriving foreign-flagged cargo ships, their management and ownership, their arrival terminal operator and owner, and rewarding proven legitimate operators with an economic incentive for their transparency will simultaneously improve port security and maritime transportation efficiencies.

  8. Classroom of the Sea.

    ERIC Educational Resources Information Center

    Hupper, Mary Laporta; Monte, Denise; Scheifele, Peter

    2000-01-01

    Introduces the Sea Program in which participant students were deaf and collaborated with a bioacoustician. Studies the underwater noise levels of the Gulf of Maine and the possible impacts on marine life. Explains implementing this project in the science curriculum. (YDS)

  9. The Weddell Sea

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Several large, irregularly shaped icebergs are floating in the Weddell Sea, east of the Antarctic Peninsula, in this true-color MODIS image from February 17, 2002. The location of several of the bergs has changed little over the last three months. Compared to an image acquired on November 13, 2001, the berg at the upper right of the image has spun around, but is still hanging around in the same general location. Similar slow-movers can be seen just to the east of the Larsen Ice Shelf, which hugs the eastern coast of the Peninsula. The northernmost of those two bergs is designated A38b; the southernmost one is A38a. These bergs were once part of an iceberg greater than 2,700 square miles that broke off the Ronne Ice Shelf (to the south) back in 1998. While the waters of the Weddell Sea in the area ought to be deep enough to float those bergs, it is possible that they have run aground on a topographic high, or ridge, in the sea floor. However, little is known about the underwater topography of that region, and it is also possible that the bergs are simply so massive that they resist being moved by surface wind or ocean currents. While four years might seem like a long time for an iceberg to hang around, these are certainly no record holders. A berg that broke off the Ross Ice Shelf (on the other side of Antarctica) drifted north and went aground south of Australia. That berg calved in 1987, and hasn't really moved in ten years. While the big bergs have not moved much in the span of time between these images, there is a big difference in the amount of sea ice present in the two images. In general, the rounder chunks of ice are more likely to be seasonal sea ice that forms from the freezing of sea water, while the larger, jagged-edged pieces of ice are more likely to be bergs that broke off an ice shelf at the margin of the continent. It's the height of summer in Antarctica in the February image, and much of the sea ice has melted or drifted away, leaving a relatively large expanse of clear ocean. Credit:

  10. Northern Sand Sea

    NASA Technical Reports Server (NTRS)

    2005-01-01

    [figure removed for brevity, see original site]

    Our topic for the weeks of April 4 and April 11 is dunes on Mars. We will look at the north polar sand sea and at isolated dune fields at lower latitudes. Sand seas on Earth are often called 'ergs,' an Arabic name for dune field. A sand sea differs from a dune field in two ways: 1) a sand sea has a large regional extent, and 2) the individual dunes are large in size and complex in form.

    This VIS image was taken at 82 degrees North latitude during Northern spring. The image is completely dominated by dunes. In sand seas, it is very common for a single type of dune to occur, and for a single predominate wind to control the alignment of the dunes.

    Image information: VIS instrument. Latitude 82.2, Longitude 152.5 East (207.5 West). 19 meter/pixel resolution.

    Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time.

    NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the California Institute of Technology in Pasadena.

  11. Biodiversity of the Deep-Sea Benthic Fauna in the Sangihe-Talaud Region, Indonesia: Observations from the INDEX-SATAL 2010 Expedition

    NASA Astrophysics Data System (ADS)

    Herrera, S.; Munro, C.; Nganro, N.; Tunnicliffe, V.; Wirasantosa, S.; Sibert, E.; Hammond, S. R.; Bors, E.; Butterfield, D.; Holden, J. F.; Baker, E. T.; Sherrin, J.; Makarim, S.; Troa, R.; Shank, T. M.

    2010-12-01

    The benthic ecosystems found in the deep-sea promontories of Sangihe Talaud region were explored, between June and August 2010, using the ROV Little Hercules aboard the NOAA ship Okeanos Explorer. The Sangihe-Talaud region is part of the Coral Triangle (CT) an area known for harboring the most biodiverse shallow-water coral reefs in the world. Notwithstanding the significant research efforts that have been undertaken to catalog and protect the biodiversity of the CT prior this expedition, virtually nothing was known about the life inhabiting the deep sea. The high-resolution imagery obtained from the 27 ROV dives revealed remarkably high abundances and diversity of animal species, many of which appear to be novel. On hard bottom substrates, cold-water corals were the dominant sessile macrofauna, in terms of biomass, followed by glass sponges (Hexactinellida) and sea lilies (Crinoidea). The coral taxa observed in this area represent six large orders of cnidarians: antipatharians (black corals), scleractinians (stony corals), zoanthideans (gold corals), alcyonaceans (octocorals), pennatulaceans (sea pens), and anthoathecates (hydrocorals). Most sessile species, independently of their size class or taxonomic affiliation, harbor a wide variety of associated fauna. Brittle stars (Ophiuroidea), squat lobsters (Galatheoidea), shrimp (Caridea), amphipods (Amphipoda), anemones (Actinaria), zanthideans, barnacles (Cirripedia), hydroids (Hydrozoa) and worms (Polychaeta) are the animal groups most commonly found forming these associations. In contrast, soft bottom habitats were dominated by stalked sponges, sea pens, sea cucumbers (Holothuroidea) and brittle stars. Other conspicuous fauna include fish, hermit crabs (Paguridae), urchins (Echinoidea) and octopuses (Cephalopoda). The abundance of habitats generated by the high number of geological and biological features and depth ranges present in the deep coral triangle (e.g., ridges, seamounts, island margins, plains, and rock types), and the complex history of tectonic dynamics of this region are among the likely causes for the high biodiversity found during this mission. Tectonic history and diverse habitats may also be factors that have played a similar role shaping the diversity of shallow water assemblages of the region. Such parallels between the biodiversity of deep and shallow waters will be presented.

  12. Sea-Level Projections from the SeaRISE Initiative

    NASA Technical Reports Server (NTRS)

    Nowicki, Sophie; Bindschadler, Robert

    2011-01-01

    SeaRISE (Sea-level Response to Ice Sheet Evolution) is a community organized modeling effort, whose goal is to inform the fifth IPCC of the potential sea-level contribution from the Greenland and Antarctic ice sheets in the 21st and 22nd century. SeaRISE seeks to determine the most likely ice sheet response to imposed climatic forcing by initializing an ensemble of models with common datasets and applying the same forcing to each model. Sensitivity experiments were designed to quantify the sea-level rise associated with a change in: 1) surface mass balance, 2) basal lubrication, and 3) ocean induced basal melt. The range of responses, resulting from the multi-model approach, is interpreted as a proxy of uncertainty in our sea-level projections. http://websrv.cs .umt.edu/isis/index.php/SeaRISE_Assessment.

  13. North Sea Sediment Dynamics

    NASA Astrophysics Data System (ADS)

    Paetsch, Johannes; Kuehn, Wilfried; Serna, Alexandra; Lahajnar, Niko; Emeis, Kay

    2014-05-01

    The North Sea is a semi-enclosed shelf sea attached to the North-east Atlantic and in its south-eastern part receives significant matter from river discharge and atmospheric deposition: Anthropogenic nutrients, dissolved carbon and organic compounds have elevated pristine background concentrations in the coastal strip of the North Sea since about 250 years as a consequence of intensified agriculture and industrialisation since then, In such a marine environment the legacy of old inputs and the influence of processes in the sediment on the biogeochemistry of the overlying water column increases with decreasing water depth. On the other hand the flux of particulate organic matter into the surface sediment (~15 cm depth) also increases with decreasing water depth or distance from the coast. Both effects are also described by results of ECOHAM-M simulations, the coupled pelagic-benthic ecosystem model that includes a prognostic multi-layer sediment module adopted from the global biogeochemical model HAMOCC as part of the MPI-earth-system model (MPI-ESM).The transfer from deep sea to coastal applications required different adaptations, such as changes in porosity values, in dissolution and organic matter remineralisation rates, and stoichiometries. In the model, two pelagic detritus pools, one slowly sinking with semi-labile organic matter, and one fast sinking with labile organic matter are collected in one sediment pool. After a spin-up of several hundred years the 3d-coupled model for the southern and central North Sea attains equilibrium, where the local inputs and outputs of the pelagic and benthic module are balanced. From this equilibrium state we initialised a realistic decadal run (2000-2009). Analysing the seasonal and interannual model results we found that the variability of sediment efflux in the model is low compared to the more dynamic pelagic system components, but simulated (and observed) sediment pore water profiles clearly show concentration gradients from the coast into the central North Sea. Data on organic matter concentration and quality in surface sediments along a comparable transect suggest that not only the loading with POM decreases with distance to land, but also the reactivity of organic matter, indicated by amino acid composition as a measure of the degree of protein degradation. This ageing or degradation effect can also be seen in a near-shore surface sediment core where the C/N ratio increases with depth, while the relative concentration of labile material in sedimentary organic matter decreases. We discuss how the quality of organic particulate matter could be integrated into the sediment model equations.

  14. Marginal seas—Terminological crisis

    NASA Astrophysics Data System (ADS)

    Mazarovich, A. O.

    2011-07-01

    The terms marginal sea, peripheral sea, and backarc sea are widely used in the contemporary Russian geological literature as synonyms but do not have, in my opinion, unequivocal treatment. The application of the term marginal sea is briefly discussed. The seas of the Pacific transitional zone are reviewed. It is proposed to define a marginal sea as a marine basin a few thousand kilometers in extent and connected with the open ocean. Domains underlain by crust of the continental and oceanic types must coexist therein. The domains with oceanic crust are expressed in the topography as deepwater basins (one or several), where fragments of continental crust may also occur. A marginal sea must be bounded by at least one island arc.

  15. The North Sea - A shelf sea in the Anthropocene

    NASA Astrophysics Data System (ADS)

    Emeis, Kay-Christian; van Beusekom, Justus; Callies, Ulrich; Ebinghaus, Ralf; Kannen, Andreas; Kraus, Gerd; Kröncke, Ingrid; Lenhart, Hermann; Lorkowski, Ina; Matthias, Volker; Möllmann, Christian; Pätsch, Johannes; Scharfe, Mirco; Thomas, Helmuth; Weisse, Ralf; Zorita, Eduardo

    2015-01-01

    Global and regional change clearly affects the structure and functioning of ecosystems in shelf seas. However, complex interactions within the shelf seas hinder the identification and unambiguous attribution of observed changes to drivers. These include variability in the climate system, in ocean dynamics, in biogeochemistry, and in shelf sea resource exploitation in the widest sense by societies. Observational time series are commonly too short, and resolution, integration time, and complexity of models are often insufficient to unravel natural variability from anthropogenic perturbation. The North Sea is a shelf sea of the North Atlantic and is impacted by virtually all global and regional developments. Natural variability (from interannual to multidecadal time scales) as response to forcing in the North Atlantic is overlain by global trends (sea level, temperature, acidification) and alternating phases of direct human impacts and attempts to remedy those. Human intervention started some 1000 years ago (diking and associated loss of wetlands), expanded to near-coastal parts in the industrial revolution of the mid-19th century (river management, waste disposal in rivers), and greatly accelerated in the mid-1950s (eutrophication, pollution, fisheries). The North Sea is now a heavily regulated shelf sea, yet societal goals (good environmental status versus increased uses), demands for benefits and policies diverge increasingly. Likely, the southern North Sea will be re-zoned as riparian countries dedicate increasing sea space for offshore wind energy generation - with uncertain consequences for the system's environmental status. We review available observational and model data (predominantly from the southeastern North Sea region) to identify and describe effects of natural variability, of secular changes, and of human impacts on the North Sea ecosystem, and outline developments in the next decades in response to environmental legislation, and in response to increased use of shelf sea space.

  16. The Aral Sea Disaster

    NASA Astrophysics Data System (ADS)

    Micklin, Philip

    2007-05-01

    The Aral Sea is a huge terminal lake located among the deserts of Central Asia. Over the past 10 millennia, it has repeatedly filled and dried, owing both to natural and human forces. The most recent desiccation started in the early 1960s and owes overwhelmingly to the expansion of irrigation that has drained its two tributary rivers. Lake level has fallen 23 m, area shrunk 74%, volume decreased 90%, and salinity grew from 10 to more than 100g/l, causing negative ecological changes, including decimation of native fish species, initiation of dust/salt storms, degradation of deltaic biotic communities, and climate change around the former shoreline. The population residing around the lake has also been negatively impacted. There is little hope in the foreseeable future to fully restore the Aral Sea, but measures to preserve/rehabilitate parts of the water body and the deltas are feasible.

  17. Establishment of primary cell culture from the temperate symbiotic cnidarian, Anemonia viridis.

    PubMed

    Barnay-Verdier, Stéphanie; Dall'osso, Diane; Joli, Nathalie; Olivré, Juliette; Priouzeau, Fabrice; Zamoum, Thamilla; Merle, Pierre-Laurent; Furla, Paola

    2013-10-01

    The temperate symbiotic sea anemone Anemonia viridis, a member of the Cnidaria phylum, is a relevant experimental model to investigate the molecular and cellular events involved in the preservation or in the rupture of the symbiosis between the animal cells and their symbiotic microalgae, commonly named zooxanthellae. In order to increase research tools for this model, we developed a primary culture from A. viridis animal cells. By adapting enzymatic dissociation protocols, we isolated animal host cells from a whole tentacle in regeneration state. Each plating resulted in a heterogeneous primary culture consisted of free zooxanthellae and many regular, small rounded and adherent cells (of 3-5 ?m diameter). Molecular analyses conducted on primary cultures, maintained for 2 weeks, confirmed a specific signature of A. viridis cells. Further serial dilutions and micromanipulation allowed us to obtain homogenous primary cultures of the small rounded cells, corresponding to A. viridis "epithelial-like cells". The maintenance and the propagation over a 4 weeks period of primary cells provide, for in vitro cnidarian studies, a preliminary step for further investigations on cnidarian cellular pathways notably in regard to symbiosis interactions. PMID:23595421

  18. The mitochondrial 60-kDa heat shock protein in marine invertebrates: biochemical purification and molecular characterization.

    PubMed

    Choresh, Omer; Loya, Yossi; Müller, Werner E G; Wiedenmann, Jörg; Azem, Abdussalam

    2004-03-01

    Sessile marine invertebrates undergo constant direct exposure to the surrounding environmental conditions, including local and global environmental fluctuations that may lead to fatal protein damage. Induction of heat shock proteins (Hsps) constitutes an important defense mechanism that protects these organisms from deleterious stress conditions. In a previous study, we reported the immunological detection of a 60-kDa Hsp (Hsp60) in the sea anemone Anemonia viridis (formerly called Anemonia sulcata) and studied its expression under a variety of stress conditions. In the present study, we show that the sponge Tetilla sp. from tidal habitats with a highly variable temperature regime is characterized by an increased level of Hsp60. Moreover, we show the expression of Hsp60 in various species among Porifera and Cnidaria, suggesting a general importance of this protein among marine invertebrates. We further cloned the hsp60 gene from A viridis, using a combination of conventional protein isolation methods and screening of a complementary deoxyribonucleic acid library by polymerase chain reaction. The cloned sequence (1764 bp) encodes for a protein of 62.8 kDa (588 amino acids). The 62.8-kDa protein, which contains an amino terminal extension that may serve as a mitochondrial targeting signal, shares a significant identity with mitochondrial Hsp60s from several animals but less identity with Hsp60s from either bacteria or plants. PMID:15270076

  19. An ancient role for nuclear beta-catenin in the evolution of axial polarity and germ layer segregation.

    PubMed

    Wikramanayake, Athula H; Hong, Melanie; Lee, Patricia N; Pang, Kevin; Byrum, Christine A; Bince, Joanna M; Xu, Ronghui; Martindale, Mark Q

    2003-11-27

    The human oncogene beta-catenin is a bifunctional protein with critical roles in both cell adhesion and transcriptional regulation in the Wnt pathway. Wnt/beta-catenin signalling has been implicated in developmental processes as diverse as elaboration of embryonic polarity, formation of germ layers, neural patterning, spindle orientation and gap junction communication, but the ancestral function of beta-catenin remains unclear. In many animal embryos, activation of beta-catenin signalling occurs in blastomeres that mark the site of gastrulation and endomesoderm formation, raising the possibility that asymmetric activation of beta-catenin signalling specified embryonic polarity and segregated germ layers in the common ancestor of bilaterally symmetrical animals. To test whether nuclear translocation of beta-catenin is involved in axial identity and/or germ layer formation in 'pre-bilaterians', we examined the in vivo distribution, stability and function of beta-catenin protein in embryos of the sea anemone Nematostella vectensis (Cnidaria, Anthozoa). Here we show that N. vectensis beta-catenin is differentially stabilized along the oral-aboral axis, translocated into nuclei in cells at the site of gastrulation and used to specify entoderm, indicating an evolutionarily ancient role for this protein in early pattern formation. PMID:14647383

  20. Evolutionary conservation of the eumetazoan gene regulatory landscape

    PubMed Central

    Schwaiger, Michaela; Schönauer, Anna; Rendeiro, André F.; Pribitzer, Carina; Schauer, Alexandra; Gilles, Anna F.; Schinko, Johannes B.; Renfer, Eduard; Fredman, David; Technau, Ulrich

    2014-01-01

    Despite considerable differences in morphology and complexity of body plans among animals, a great part of the gene set is shared among Bilateria and their basally branching sister group, the Cnidaria. This suggests that the common ancestor of eumetazoans already had a highly complex gene repertoire. At present it is therefore unclear how morphological diversification is encoded in the genome. Here we address the possibility that differences in gene regulation could contribute to the large morphological divergence between cnidarians and bilaterians. To this end, we generated the first genome-wide map of gene regulatory elements in a nonbilaterian animal, the sea anemone Nematostella vectensis. Using chromatin immunoprecipitation followed by deep sequencing of five chromatin modifications and a transcriptional cofactor, we identified over 5000 enhancers in the Nematostella genome and could validate 75% of the tested enhancers in vivo. We found that in Nematostella, but not in yeast, enhancers are characterized by the same combination of histone modifications as in bilaterians, and these enhancers preferentially target developmental regulatory genes. Surprisingly, the distribution and abundance of gene regulatory elements relative to these genes are shared between Nematostella and bilaterian model organisms. Our results suggest that complex gene regulation originated at least 600 million yr ago, predating the common ancestor of eumetazoans. PMID:24642862

  1. An ancient role for nuclear beta-catenin in the evolution of axial polarity and germ layer segregation

    NASA Technical Reports Server (NTRS)

    Wikramanayake, Athula H.; Hong, Melanie; Lee, Patricia N.; Pang, Kevin; Byrum, Christine A.; Bince, Joanna M.; Xu, Ronghui; Martindale, Mark Q.

    2003-01-01

    The human oncogene beta-catenin is a bifunctional protein with critical roles in both cell adhesion and transcriptional regulation in the Wnt pathway. Wnt/beta-catenin signalling has been implicated in developmental processes as diverse as elaboration of embryonic polarity, formation of germ layers, neural patterning, spindle orientation and gap junction communication, but the ancestral function of beta-catenin remains unclear. In many animal embryos, activation of beta-catenin signalling occurs in blastomeres that mark the site of gastrulation and endomesoderm formation, raising the possibility that asymmetric activation of beta-catenin signalling specified embryonic polarity and segregated germ layers in the common ancestor of bilaterally symmetrical animals. To test whether nuclear translocation of beta-catenin is involved in axial identity and/or germ layer formation in 'pre-bilaterians', we examined the in vivo distribution, stability and function of beta-catenin protein in embryos of the sea anemone Nematostella vectensis (Cnidaria, Anthozoa). Here we show that N. vectensis beta-catenin is differentially stabilized along the oral-aboral axis, translocated into nuclei in cells at the site of gastrulation and used to specify entoderm, indicating an evolutionarily ancient role for this protein in early pattern formation.

  2. Sterol patterns of cultured zooxanthellae isolated from marine invertebrates: Synthesis of gorgosterol and 23-desmethylgorgosterol by aposymbiotic algae

    PubMed Central

    Withers, Nancy W.; Kokke, W. C. M. C.; Fenical, William; Djerassi, Carl

    1982-01-01

    Quantitative sterol compositions of cultured zooxanthellae isolated from various Pacific and Atlantic invertebrate hosts: Zoanthus sociatus (a zoanthid), Oculina diffusa (a scleractian coral), Tridacna gigas (a giant clam), Melibe pilosa (a nudibranch), and Aiptasia pulchella (a sea anemone) are reported. The results clearly demonstrate large differences in sterol patterns of zooxanthellae and that there is no obvious relationship between the taxonomic affiliation of the host and the sterol pattern of its isolated symbiont. The sterols of the zooxanthellae of O. diffusa (Cnidaria) and T. gigas (Mollusca) are qualitatively equivalent. Based on the structures of the two major free sterols synthesized by each alga, the zooxanthellae from different hosts were separated into three distinct groups. It was also found that an aposymbiotic alga can synthesize the unique marine sterols gorgosterol and 23-desmethylgorgosterol. Most of the sterols were identified by using mass spectroscopy and 360-MHz proton magnetic resonance. Spectroscopic data are reported for four novel sterols—(23,24R)-dimethyl-5α-cholest-(22E)-en-3β-o l, 23-methyl-5α-cholest-22E-en-3β-ol, cholesta-5,14-dien-3β-ol, and 4α-methyl-5α-cholesta-8(14)-24-dien-3β-ol. PMID:16593195

  3. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer.

    PubMed

    Hashimshony, Tamar; Feder, Martin; Levin, Michal; Hall, Brian K; Yanai, Itai

    2015-03-12

    The concept of germ layers has been one of the foremost organizing principles in developmental biology, classification, systematics and evolution for 150 years (refs 1 - 3). Of the three germ layers, the mesoderm is found in bilaterian animals but is absent in species in the phyla Cnidaria and Ctenophora, which has been taken as evidence that the mesoderm was the final germ layer to evolve. The origin of the ectoderm and endoderm germ layers, however, remains unclear, with models supporting the antecedence of each as well as a simultaneous origin. Here we determine the temporal and spatial components of gene expression spanning embryonic development for all Caenorhabditis elegans genes and use it to determine the evolutionary ages of the germ layers. The gene expression program of the mesoderm is induced after those of the ectoderm and endoderm, thus making it the last germ layer both to evolve and to develop. Strikingly, the C. elegans endoderm and ectoderm expression programs do not co-induce; rather the endoderm activates earlier, and this is also observed in the expression of endoderm orthologues during the embryology of the frog Xenopus tropicalis, the sea anemone Nematostella vectensis and the sponge Amphimedon queenslandica. Querying the phylogenetic ages of specifically expressed genes reveals that the endoderm comprises older genes. Taken together, we propose that the endoderm program dates back to the origin of multicellularity, whereas the ectoderm originated as a secondary germ layer freed from ancestral feeding functions. PMID:25487147

  4. Drillship for rough seas

    SciTech Connect

    Penn, J.T.

    1982-11-01

    PENN ENGINEERING of Orange, Texas, extending a concept previously applied to a drilling tender, has designed a pair of economical new drillships that will enlarge the weather window for drilling in many severe environments. Two factors make this possible: an external motion suppression system that reduces roll by as much as 90% and heave by as much as 40%, and A central mooring system that makes it possible to turn the vessel 90/sup 0/ in either direction to head into the seas.

  5. Dead Sea rhodopsins revisited.

    PubMed

    Bodaker, Idan; Suzuki, Marcelino T; Oren, Aharon; Béjà, Oded

    2012-12-01

    The Dead Sea is a unique hypersaline ecosystem with near toxic magnesium levels (?2?M), dominance of divalent cations and a slightly acidic pH. Previously, we reported a haloarchaeon related to Halobacterium salinarum to dominate in a microbial bloom that developed in 1992 in the upper water layers of the lake following massive freshwater runoff. Whether this clade also dominated an earlier bloom in 1980-1982 cannot be ascertained as no samples for cultivation-independent analysis were preserved. The presence of the light-driven proton pump bacteriorhodopsin was reported in the 1980-1982 bloom of prokaryotes that had developed in the Dead Sea. To test the hypothesis that bacteriorhodopsin proton pumping may play a major role in determining what type of haloarchaea may dominate in specific bloom conditions, we compared rhodopsin genes recovered from Dead Sea biomass collected in different periods with genes coding for retinal proteins in isolated haloarchaea. Novel bacteriorhodopsin and sensory rhodopsin genes were found in samples collected in 2007 and 2010. The fact that no rhodopsin genes were recovered from samples collected during the 1992 bloom, which was dominated by a single species, suggests that different clades were present in the 1980-1982 and 1992 blooms, and that bacteriorhodopsin proton pumping did not necessarily play a determinative role in the dominance of specific halophiles in the blooms. PMID:23760932

  6. Curonian Spit, Baltic Sea

    NASA Technical Reports Server (NTRS)

    2006-01-01

    On July 25, 2006, the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), flying on NASA's Terra satellite, captured this image of the Curonian (or Courland)spit, the Curonian Lagoon (also known as the Courland Lagoon) it encloses, and part of the Baltic Sea. Just 3,800 meters (about 12,460 feet) at its widest point, the spit encloses a lagoon of some 1,620 square kilometers (625 square miles). In this image, dark blue indicates deep water, and lighter blue indicates shallow and/or sediment-laden water. Different shades of blue distinguish the deeper Baltic Sea and the shallower Curonian Lagoon. Vegetation appears in varying shades of green, paved surfaces and bare ground appear in shades of beige and gray, and sandy areas appear off-white. Obvious sandy areas appear along the length of the spit. On the Baltic Sea side, a thin off-white band of beach runs the length of the spit; on the Curonian Lagoon side, intermittent beaches carve their way into the narrow strip of land.

  7. Kara Sea radioactivity assessment.

    PubMed

    Osvath, I; Povinec, P P; Baxter, M S

    1999-09-30

    Investigations following five international expeditions to the Kara Sea have shown that no radiologically significant contamination has occurred outside of the dumping sites in Novaya Zemlya bays. Increased levels of radionuclides in sediment have only been observed in Abrosimov and Stepovoy Bays very close to dumped containers. Evaluations of radionuclide inventories in water and sediment of the open Kara Sea and Novaya Zemlya bays as well as soil from the shore of Abrosimov bay have shown that radionuclide contamination of the open Kara Sea is mainly due to global fallout, with smaller contributions from the Sellafield reprocessing plant, the Chernobyl accident run-off from the Ob and Yenisey rivers and local fallout. Computer modelling results have shown that maximum annual doses of approximately 1 mSv are expected for a hypothetical critical group subsisting on fish caught in the Novaya Zemlya bays whereas populations living on the mainland can be expected to receive doses at least three orders of magnitude lower. PMID:10568274

  8. Stylasteridae (Cnidaria, Hydrozoa, Filifera) from South Africa.

    PubMed

    Cairns, Stephen D; Zibrowius, Helmut

    2013-01-01

    We describe and figure the 20 species of stylasterid hydrozoans known from South Africa. These consist of seven new species, six range extensions, and seven species previously known from South African. Most of the specimens reported resulted from expeditions of the Pieter Faure (1898-1903) and Meiring Naude (1975-1987), and are deposited primarily in the South African Museum (Cape Town). A replacement name (Errina australis) is proposed for the junior homonym Errina hicksoni Cairns, 1991. A brief history of species discovery of the South African stylasterids is presented. The new morphological term dactyloglossa is introrduced to define a baffle-like structure found in dactylopores of some species. Of the 20 South African species, 12 (60%) are not yet known outside that area. Three patterns of distribution were noted: species confined to the tropical region of South Africa, those found in both the tropical and warm temperate regions of S. Africa, and those found only in the warm temperate regions, three in the latter group sharing their distribution with cold temperate southern South America and two with warm temperate New Zealand, representing a circum-southern temperate distribution. PMID:26167568

  9. Water Mass Properties and Distribution between South China Sea, Sulu Sea and Celebes Sea

    NASA Astrophysics Data System (ADS)

    Fadzil Akhir, Mohd; Arsad, Shukri

    2013-04-01

    A definition of water mass properties, characteristics and its origin along the coast of northern Borneo are presented based on 55 CTD casts cruises in July 2009 combined with five Argo profiling floats at surrounding seas. The T-S relation in the study area, which includes South China Sea, Sulu Sea and Celebes Sea, show existences of eight water masses. In reference of earlier studies, we define water masses in the surface mixed layer with strong mixed of Open Sea Water (OSW), Continental Shelf Water (CSW) and Tropical Surface Water (TSW). Below the layer of this active mixing is a zone of rapid transition called the Seasonal Thermocline Water (STW). Meanwhile, the Maximum Salinity Water (MSW), Permanent Thermocline Water (PTW), North Pacific Intermediate Water (NPIW) and Deep Water (DW) were found at the depth range from below the seasonal thermocline to about 1000 m. In addition, study of BRAN (BLUElink ReANalysis) global ocean model was conducted to demonstrate how current circulation influence the exchange of water mass between the 3 seas. Sulu Sea sits in the middle and has very limited connection between the other two seas. Connection with Celebes Sea occurs at the inlet of 200m depth. Water exchange happens in two ways; surface inflow and subsurface outflow. While in South China Sea, inlet is limited to 50m depth and surface flow is mostly dominant. The current circulation of the adjacent sea demonstrate some of the water mass were origiated as far as north South China Sea and Pacific Ocean. Water mass differences between the seas were further classified to distinguish dissimilarities and define the origin the difference. Given the unique geographical background and current circulation of the area, the characteristics of the interaction between water mass distribution and current circulation has provide important overview to the area which previously not well understood.

  10. Sea Salt Source Function over the Baltic Sea

    NASA Astrophysics Data System (ADS)

    Petelski, Tomasz; Makuch, Przemyslaw; Markuszewski, Piotr; Jankowski, Andrzej; Zieli?ski, Tymon

    2013-04-01

    Studies of production and transport of aerosol over the sea are very important for many areas of knowledge. Marine aerosol emitted from the sea surface helps to clean the boundary layer from other aerosol particles. The emitted droplets do not dry out in the highly humid surface layer air and because of their sizes most of them are deposited quickly at the sea surface. Therefore, marine aerosol has many features of rain i.e. the deposition in the marine boundary layer in high wind events is controlled not only by the "dry" processes but also by the "wet" scavenging. While many cruises conducted on board S/Y Oceania, we collected many data which were used to calculate sea salt source function over the Baltic Sea. Our cruises held between 2009 and 2012. Measurements were carried out using gradient method. For this method we used Laser Particle Counter (PMS model CSASP-100_HV) placed on one oft the mast of S/Y Oceania. Measurements were performed on five different levels around sea level: 8, 11, 14, 17 and 20 meters. Based on the averaged vertical concentration, profiles were calculated, using Monin-Obuchow theory, vertical sea spray fluxes in the near water layer. Based on fluxes calculated from vertical concentration profiles was calculated sea salt source function over the Baltic Sea. This function gives emission for different particle size, depending on environmental parameters. Emission of sea spray depends of the size of energy lost by the wind waves in process of collapse. Acknowledgements: The support for this study was provided by the project Satellite Monitoring of the Baltic Sea Environment - SatBa?tyk founded by European Union through European Regional Development Fund contract No. POIG 01.01.02-22-011/09.

  11. Experiencing the Full Research Process at Sea Education Association (SEA)

    NASA Astrophysics Data System (ADS)

    Harris, S. E.; Joyce, P.; Jaroslow, G.; Graziano, L.; Lea, C.; Witting, J.; Bower, A.

    2003-12-01

    While some undergraduate research experiences include only a small piece of the research process, students attending Sea Education Association's SEA Semester complete all aspects of oceanographic research in an intensive 12 week program that earns a full semester's credit. In the first half of the program, students read and discuss background literature on a subject, ask questions, pose hypotheses, and develop a written research proposal, which they defend orally. The second half of the course takes place at sea on one of SEA's state-of-the-art oceanographic research vessels where students carry out their sampling plans, analyze samples and data, write a final paper and present their results before the vessel reaches port, completing the course. At sea, students participate in sample collection and analysis for all student projects in addition to learning the general oceanography along their cruise track. This structure exposes students to the realities of research from start to finish and allows them to take full ownership of their projects. In addition to honing writing, public speaking, and problem-solving skills, students learn that research requires dedication, flexibility, and creativity, particularly when their results are unexpected or negate their hypothesis. SEA's undergraduate research program has been developing since 1971. Over that time, SEA has collected an extensive historical oceanographic database in the western Atlantic and Caribbean, plus Pacific data since 2001. This database is available to both students and outside research scientists. Collaborations with scientists outside SEA enhance the student experience and help facilitate oceanographic research by providing "ship-of-opportunity" sampling in remote locations. SEA Semester provides an excellent model for undergraduate research experiences with over 5000 alumni, about 30% of whom enter graduate school. About half the students in SEA's undergraduate programs are non-science majors. Although their experience at SEA may be their only hands-on exposure to scientific research, they take away an understanding of the process and an ability to think critically about scientific problems.

  12. Regime shifts in North Sea and Baltic Sea: A comparison

    NASA Astrophysics Data System (ADS)

    Dippner, Joachim W.; Möller, Caroline; Hänninen, Jari

    2012-12-01

    The ICES subdivisions in the North Sea (SD IIIa, SD IVa, and SD IVb) and the subdivisions in the Baltic Sea (SD 29, SD 27/28-2, and SD 25/26) are selected to compare the response in long term monitoring data (1970-2000) with respect to climate regime shifts. A modified AMOEBA model is applied to the data sets to identify the status and development of the North Sea and Baltic Sea system during two recent regime shifts. Biological regime shifts can be identified 1989/1990 in SD IIIa in the North Sea and in SD 25/26 in the Baltic Sea. A synchronous appearance of regime shifts could only be identified in the central and southern Baltic Sea for both regime shifts 1975/76 and 1989/90 where the AMOEBA model indicated a high similarity in ecosystem response. A clear difference was identified in the response of the North Sea and the Baltic Sea. Inter-annual and inter-decadal variability as well as regime shifts are driven in the Baltic Sea by direct atmospheric forcing only. In contrast, the changes in the North Sea are influenced by both the direct atmospheric forcing and the indirect forcing from the changes in North Atlantic. The fact that regime shifts as well as their synchronous appearance can be identified with the AMOEBA model might be of major interest for the management of sustainable use of ecosystem goods and services, the development of ecosystem approach to management and the implementation of the Marine Strategy Framework Directive (MSFD) of the European Union (EU).

  13. Arctic Sea Level Since 1950

    NASA Astrophysics Data System (ADS)

    Cazenave, A. A.; Henry, O.; Prandi, P.; Llovel, W.; Jevrejeva, S.

    2011-12-01

    We investigate sea level change and variability in the Arctic region over the 1950-2009 time span. Analysis of >60 long tide gauge records available since 1950 along the Norwegian and Russian sectors shows that coastal mean sea level was almost stable until about 1980 but since then displayed a clear increasing trend. In fact until the mid-1990s, the mean sea level closely followed the Arctic Oscillation (AO) index variations. However, since about 1995, the mea sea level curve departs from the AO influence and presents a large increasing trend of ~ 4 mm/yr. Using in situ ocean temperature data down to 700 m (from the WOD09 and JAMSTEC data bases), we estimated the thermosteric sea level at the tide gauge sites of the Norwegian sector and found that the recent increase in sea level has a dominant thermal origin. This suggests that inflow of warm North Atlantic waters may be responsible for the recent sea level changes observed along the Norwegian and Russian coasts. Comparison, over the altimetry era (since 1993), of altimetry-based and steric spatial trend patterns in sea level in the North Atlantic (>50°N) and Nordic Seas shows on the other hand that observed (altimetry-based) patterns essentially result from a combination of temperature and salinity effects, themselves driven by natural climate modes (AO and NAO).

  14. Sea Otter, River Otter. The Wonder Series.

    ERIC Educational Resources Information Center

    Robinson, Sandra Chisholm

    This curriculum guide is all about otters and provides information on both sea and river otters. Included are activities related to the diet of sea otters, the adaptations sea otters have made to live in the sea, their tool-using abilities, where they live and how to spot them, comparative anatomy of sea and river otters, and otter movement. The…

  15. Sea Otter, River Otter. The Wonder Series.

    ERIC Educational Resources Information Center

    Robinson, Sandra Chisholm

    This curriculum guide is all about otters and provides information on both sea and river otters. Included are activities related to the diet of sea otters, the adaptations sea otters have made to live in the sea, their tool-using abilities, where they live and how to spot them, comparative anatomy of sea and river otters, and otter movement. The…

  16. The USGS Salton Sea Science Office

    USGS Publications Warehouse

    Case, Harvey Lee, III; Barnum, Douglas A.

    2007-01-01

    The U.S. Geological Survey's (USGS) Salton Sea Science Office (SSSO) provides scientific information and evaluations to decisionmakers who are engaged in restoration planning and actions associated with the Salton Sea. The primary focus is the natural resources of the Salton Sea, including the sea?s ability to sustain biological resources and associated social and economic values.

  17. The Sea Around Us

    NASA Astrophysics Data System (ADS)

    Carson, Rachel L.

    1991-12-01

    Published in 1951, The Sea Around Us is one of the most remarkably successful books ever written about the natural world. Rachel Carson's rare ability to combine scientific insight with moving, poetic prose catapulted her book to first place on The New York Times best-seller list, where it enjoyed wide attention for thirty-one consecutive weeks. It remained on the list for more than a year and a half and ultimately sold well over a million copies, has been translated into 28 languages, inspired an Academy Award-winning documentary, and won both the 1952 National Book Award and the John Burroughs Medal. This classic work remains as fresh today as when it first appeared. Carson's writing teems with stunning, memorable images--the newly formed Earth cooling beneath an endlessly overcast sky; the centuries of nonstop rain that created the oceans; giant squids battling sperm whales hundreds of fathoms below the surface; and incredibly powerful tides moving 100 billion tons of water daily in the Bay of Fundy. Quite simply, she captures the mystery and allure of the ocean with a compelling blend of imagination and expertise. Reintroducing a classic work to a whole new generation of readers, this Special Edition features a new chapter written by Jeffrey Levinton, a leading expert in marine ecology, that brings the scientific side of The Sea Around Us completely up to date. Levinton incorporates the most recent thinking on continental drift, coral reefs, the spread of the ocean floor, the deterioration of the oceans, mass extinction of sea life, and many other topics. In addition, acclaimed nature writer Ann Zwinger has contributed a brief foreword. Today, with the oceans endangered by the dumping of medical waste and ecological disasters such as the Exxon oil spill in Alaska, this illuminating volume provides a timely reminder of both the fragility and the importance of the ocean and the life that abounds within it. Anyone who loves the sea, or who is concerned about our natural environment, will want to read this classic work.

  18. Two Sea-Level Challenges

    NASA Astrophysics Data System (ADS)

    Galvin, C.

    2008-12-01

    "No place on the sandy ocean shores of the world has been shown to be eroding because of sea level rise." This statement appeared nearly 19 years ago in bold print at the top of the page in a brief article published in Shore and Beach (Galvin,1990). The term "sea level rise" was defined in 1990 as follows: "In this statement, "sea level rise" has the meaning that the average person on the street usually attaches to that term. That is, sea level is rising; not, as in some places like the Mississippi River delta, land level is sinking." While still a subject of controversy, it is now (2008) increasingly plausible (Tornqvist et al,2008) that damage from Hurricane Katrina was significantly worse on the Mississippi River delta because floodwaters exploited wetlands and levees whose elevations had been lowered by decades of compaction in the underlying soil. (1) "Sea level" commonly appears in the literature as "relative sea level rise", occurring that way in 711 publications between 1980 and 2009 (GeoRef database on 8 Sep 08). "Relative sea level rise" does not appear in the 2005 AGI Glossary. The nearest Glossary term is "relative change in sea level", but that term occurs in only 12 publications between 1980 and 2009. The Glossary defines this term in a sequence stratigraphy sense, which infers that "relative sea level rise" is the sum of bottom subsidence and eustatic sea level rise. In plain English, "relative sea level rise" means "water depth increase". For present day coastal environments, "relative sea level rise" is commonly used where eustatic sea level rise is less than subsidence, that is, where the magnitude of actual sea level rise is smaller than the magnitude of subsidence. In that situation, "relative sea level rise" misleads both the average person and the scientist who is not a coastal geologist. Thus, the first challenge is to abandon "relative sea level rise" in favor of "water depth increase", in order that the words accurately descibe what happens. It would further clarify popular understanding if the term "actual sea level rise" were used in place of "eustatic sea level rise". (2)Geologists have approximated the the practice of paleontologists and biologists in establishing type examples of important geological features. This is a useful practice. A graduate geologist holds in mind clear conceptions of "beach cusps", "drumlin fields", "birdfoot deltas", and "igneous sills" based on seeing field examples accepted by professional geologists as representative of these features. However, although publications frequently report that sea level rise erodes a particular beach, no one identifies a type beach where that cause has been proven to produce the alleged effect. At the type beach, it is necessary to show that sea level is rising, and that the beach erodes primarily from this sea level rise, rather than from interrupted longshore transport. Thus, the second challenge is to identify a type ocean beach proven to erode because of sea level rise.

  19. Holocene sea surface temperature and sea ice extent in the Okhotsk and Bering Seas

    NASA Astrophysics Data System (ADS)

    Harada, Naomi; Katsuki, Kota; Nakagawa, Mitsuhiro; Matsumoto, Akiko; Seki, Osamu; Addison, Jason A.; Finney, Bruce P.; Sato, Miyako

    2014-08-01

    Accurate prediction of future climate requires an understanding of the mechanisms of the Holocene climate; however, the driving forces, mechanisms, and processes of climate change in the Holocene associated with different time scales remain unclear. We investigated the drivers of Holocene sea surface temperature (SST) and sea ice extent in the North Pacific Ocean, and the Okhotsk and Bering Seas, as inferred from sediment core records, by using the alkenone unsaturation index as a biomarker of SST and abundances of sea ice-related diatoms (F. cylindrus and F. oceanica) as an indicator of sea ice extent to explore controlling mechanisms in the high-latitude Pacific. Temporal changes in alkenone content suggest that alkenone production was relatively high during the middle Holocene in the Okhotsk Sea and the western North Pacific, but highest in the late Holocene in the eastern Bering Sea and the eastern North Pacific. The Holocene variations of alkenone-SSTs at sites near Kamchatka in the Northwest Pacific, as well as in the western and eastern regions of the Bering Sea, and in the eastern North Pacific track the changes of Holocene summer insolation at 50°N, but at other sites in the western North Pacific, in the southern Okhotsk Sea, and the eastern Bering Sea they do not. In addition to insolation, other atmosphere and ocean climate drivers, such as sea ice distribution and changes in the position and activity of the Aleutian Low, may have systematically influenced the timing and magnitude of warming and cooling during the Holocene within the subarctic North Pacific. Periods of high sea ice extent in both the Okhotsk and Bering Seas may correspond to some periods of frequent or strong winter-spring dust storms in the Mongolian Gobi Desert, particularly one centered at ?4-3 thousand years before present (kyr BP). Variation in storm activity in the Mongolian Gobi Desert region may reflect changes in the strength and positions of the Aleutian Low and Siberian High. We suggest that periods of eastward displacement or increased intensity of the Aleutian Low correspond with times of increased extent of sea ice in the western Okhotsk Sea and eastern Bering Sea.

  20. Sea modeling and rendering

    NASA Astrophysics Data System (ADS)

    Cathala, Thierry; Latger, Jean

    2010-10-01

    More and more defence and civil applications require simulation of marine synthetic environment. Currently, the "Future Anti-Surface-Guided-Weapon" (FASGW) or "anti-navire léger" (ANL) missile needs this kind of modelling. This paper presents a set of technical enhancement of the SE-Workbench that aim at better representing the sea profile and the interaction with targets. The operational scenario variability is a key criterion: the generic geographical area (e.g. Persian Gulf, coast of Somalia,...), the type of situation (e.g. peace keeping, peace enforcement, anti-piracy, drug interdiction,...)., the objectives (political, strategic, or military objectives), the description of the mission(s) (e.g. antipiracy) and operation(s) (e.g. surveillance and reconnaissance, escort, convoying) to achieve the objectives, the type of environment (Weather, Time of day, Geography [coastlines, islands, hills/mountains]). The paper insists on several points such as the dual rendering using either ray tracing [and the GP GPU optimization] or rasterization [and GPU shaders optimization], the modelling of sea-surface based on hypertextures and shaders, the wakes modelling, the buoyancy models for targets, the interaction of coast and littoral, the dielectric infrared modelling of water material.

  1. A Silurian sea spider.

    PubMed

    Siveter, Derek J; Sutton, Mark D; Briggs, Derek E G; Siveter, David J

    2004-10-21

    Pycnogonids (sea spiders) are marine arthropods numbering some 1,160 extant species. They are globally distributed in depths of up to 6,000 metres, and locally abundant; however, their typically delicate form and non-biomineralized cuticle has resulted in an extremely sparse fossil record that is not accepted universally. There are two opposing views of their phylogenetic position: either within Chelicerata as sister group to the euchelicerates, or as a sister taxon to all other euarthropods. The Silurian Herefordshire Konservat-Lagerstatte in England (approximately 425 million years (Myr) bp) yields exceptionally preserved three-dimensional fossils that provide unrivalled insights into the palaeobiology of a variety of invertebrates. The fossils are preserved as calcitic void in-fills in carbonate concretions within a volcaniclastic horizon, and are reconstructed digitally. Here we describe a new pycnogonid from this deposit, which is the oldest adult sea spider by approximately 35 Myr and the most completely known fossil species. The large chelate first appendage is consistent with a chelicerate affinity for the pycnogonids. Cladistic analyses place the new species near the base of the pycnogonid crown group, implying that the latter had arisen by the Silurian period. PMID:15496921

  2. Greenland Sea observations

    SciTech Connect

    Gudmandsen, P.; Mortensen, H.B.; Pedersen, L.T.; Skriver, H.; Minnett, P.

    1992-12-31

    ERS-1 SAR data have been acquired over the Greenland Sea and Fram Strait during two periods, the Ice Phase of three-day repeat cycle from January to March 1992 and a one-month period in the 35-day repeat cycle from 16 July to 15 August 1992. Most data became available by way of the Broadband Data Dissemination System, i.e. with a spatial resolution of about 100 m. With these data various algorithms have been tested to derive sea ice parameters such as ice extent, ice concentration and ice displacement. In the latter period data were collected to support the activities of a research vessel in the area mainly related to the large polynyas that form east and north of Greenland. The formation of polynyas could clearly be outlined but also other phenomena were observed related to the influence of wind streets and gravity waves associated with the atmospheric boundary layer. The data will have to be studied further including full-resolution data to substantiate the conclusions arrived at.

  3. Spectacled Eiders Wintering in Northern Bering Sea

    USGS Multimedia Gallery

    Large flock of Spectacled Eiders aggregated in a sea ice lead in the northern Bering Sea, south of St. Lawrence Island, Alaska.  Aerial photograph from a helicopter deployed from the USCG Cutter Polar Sea....

  4. Spectacled Eiders Wintering in Northern Bering Sea

    USGS Multimedia Gallery

    Large flock of Spectacled Eiders aggregated in a sea ice lead in the northern Bering Sea, south of St. Lawrence Island, Alaska.  Aerial photograph from a helicopter deployed from the USCG Cutter Polar Sea. ...

  5. Spectacled Eiders Wintering in Northern Bering Sea

    USGS Multimedia Gallery

    Large flock of Spectacled Eiders aggregated in a sea ice lead at sunset in the northern Bering Sea, south of St. Lawrence Island, Alaska.  Aerial photograph from a helicopter deployed from the USCG Cutter Polar Sea....

  6. ROC Sampling Deep Sea Urchin

    USGS Multimedia Gallery

    Images of the remotely operated vehicle Jason2 sampling a sea urchin in a deep sea mussel community found near a gas seep on the U.S. outer continental shelf. Images courtesy Deepwater Canyons 2013 - Pathways to the Abyss expedition, NOAA-OER/BOEM/USGS....

  7. Arctic Sea ice model sensitivities.

    SciTech Connect

    Peterson, Kara J.; Bochev, Pavel Blagoveston; Paskaleva, Biliana Stefanova

    2010-12-01

    Arctic sea ice is an important component of the global climate system and, due to feedback effects, the Arctic ice cover is changing rapidly. Predictive mathematical models are of paramount importance for accurate estimates of the future ice trajectory. However, the sea ice components of Global Climate Models (GCMs) vary significantly in their prediction of the future state of Arctic sea ice and have generally underestimated the rate of decline in minimum sea ice extent seen over the past thirty years. One of the contributing factors to this variability is the sensitivity of the sea ice state to internal model parameters. A new sea ice model that holds some promise for improving sea ice predictions incorporates an anisotropic elastic-decohesive rheology and dynamics solved using the material-point method (MPM), which combines Lagrangian particles for advection with a background grid for gradient computations. We evaluate the variability of this MPM sea ice code and compare it with the Los Alamos National Laboratory CICE code for a single year simulation of the Arctic basin using consistent ocean and atmospheric forcing. Sensitivities of ice volume, ice area, ice extent, root mean square (RMS) ice speed, central Arctic ice thickness,and central Arctic ice speed with respect to ten different dynamic and thermodynamic parameters are evaluated both individually and in combination using the Design Analysis Kit for Optimization and Terascale Applications (DAKOTA). We find similar responses for the two codes and some interesting seasonal variability in the strength of the parameters on the solution.

  8. Probability of sea level rise

    SciTech Connect

    Titus, J.G.; Narayanan, V.K.

    1995-10-01

    The report develops probability-based projections that can be added to local tide-gage trends to estimate future sea level at particular locations. It uses the same models employed by previous assessments of sea level rise. The key coefficients in those models are based on subjective probability distributions supplied by a cross-section of climatologists, oceanographers, and glaciologists.

  9. The Barbados Sea Level Record

    NASA Astrophysics Data System (ADS)

    Fairbanks, R. G.; Mortlock, R. A.; Abdul, N. A.; Wright, J. D.; Cao, L.; Mey, J. L.

    2013-12-01

    Additional offshore drill cores, nearly 100 new radiometric dates, and more than 1000 kilometers of Multibeam mapping greatly enhance the Barbados Sea Level record. Extensive Multibeam mapping around the entire island covers approximately 2650 km2 of the sea bottom and now integrates the offshore reef topography and Barbados Sea Level Record with the unparalleled onshore core collection, digital elevation maps, and Pleistocene sea level record spanning the past one million years. The reef crest coral, Acropora palmata, remains the stalwart indicator of sea level for many reasons that are validated by our redundant sea level records and redundant dating via Th/U and Pa/U analyses. Microanalysis and densitometry studies better explain why Acropora palmata is so well preserved in the Pleistocene reef records and therefore why it is the species of choice for sea level reconstructions and radiometric dating. New drill cores into reefs that formed during Marine Isotope Stage 3 lead us to a model of diagenesis that allows us to better prospect for unaltered coral samples in older reefs that may be suitable for Th/U dating. Equally important, our diagenesis model reinforces our rigorous sample quality criteria in a more quantitative manner. The Barbados Sea Level record has a sampling resolution of better than 100 years throughout much of the last deglaciation showing unprecedented detail in redundant drill cores. The Melt Water Pulses (MWP1A and MWP1B) are well resolved and the intervening interval that includes the Younger Dryas reveals sea level changes in new detail that are consistent with the terrestrial records of ice margins (see Abdul et al., this section). More than 100 paired Th/U and radiocarbon ages place the Barbados Sea Level Record unambiguously on the radiocarbon time scale for direct comparisons with the terrestrial records of ice margin changes.

  10. Recovery of benthic megafauna from anthropogenic disturbance at a hydrocarbon drilling well (380 m depth in the Norwegian Sea).

    PubMed

    Gates, Andrew R; Jones, Daniel O B

    2012-01-01

    Recovery from disturbance in deep water is poorly understood, but as anthropogenic impacts increase in deeper water it is important to quantify the process. Exploratory hydrocarbon drilling causes physical disturbance, smothering the seabed near the well. Video transects obtained by remotely operated vehicles were used to assess the change in invertebrate megafaunal density and diversity caused by drilling a well at 380 m depth in the Norwegian Sea in 2006. Transects were carried out one day before drilling commenced and 27 days, 76 days, and three years later. A background survey, further from the well, was also carried out in 2009. Porifera (45% of observations) and Cnidaria (40%) dominated the megafauna. Porifera accounted for 94% of hard-substratum organisms and cnidarians (Pennatulacea) dominated on the soft sediment (78%). Twenty seven and 76 days after drilling commenced, drill cuttings were visible, extending over 100 m from the well. In this area there were low invertebrate megafaunal densities (0.08 and 0.10 individuals m(-2)) in comparison to pre-drill conditions (0.21 individuals m(-2)). Three years later the visible extent of the cuttings had reduced, reaching 60 m from the well. Within this area the megafaunal density (0.05 individuals m(-2)) was lower than pre-drill and reference transects (0.23 individuals m(-2)). There was a significant increase in total megafaunal invertebrate densities with both distance from drilling and time since drilling although no significant interaction. Beyond the visible disturbance there were similar megafaunal densities (0.14 individuals m(-2)) to pre-drilling and background surveys. Species richness, Shannon-Weiner diversity and multivariate techniques showed similar patterns to density. At this site the effects of exploratory drilling on megafaunal invertebrate density and diversity seem confined to the extent of the visible cuttings pile. However, elevated Barium concentration and reduced sediment grain size suggest persistence of disturbance for three years, with unclear consequences for other components of the benthic fauna. PMID:23056177

  11. Recovery of Benthic Megafauna from Anthropogenic Disturbance at a Hydrocarbon Drilling Well (380 m Depth in the Norwegian Sea)

    PubMed Central

    Gates, Andrew R.; Jones, Daniel O. B.

    2012-01-01

    Recovery from disturbance in deep water is poorly understood, but as anthropogenic impacts increase in deeper water it is important to quantify the process. Exploratory hydrocarbon drilling causes physical disturbance, smothering the seabed near the well. Video transects obtained by remotely operated vehicles were used to assess the change in invertebrate megafaunal density and diversity caused by drilling a well at 380 m depth in the Norwegian Sea in 2006. Transects were carried out one day before drilling commenced and 27 days, 76 days, and three years later. A background survey, further from the well, was also carried out in 2009. Porifera (45% of observations) and Cnidaria (40%) dominated the megafauna. Porifera accounted for 94% of hard-substratum organisms and cnidarians (Pennatulacea) dominated on the soft sediment (78%). Twenty seven and 76 days after drilling commenced, drill cuttings were visible, extending over 100 m from the well. In this area there were low invertebrate megafaunal densities (0.08 and 0.10 individuals m−2) in comparison to pre-drill conditions (0.21 individuals m−2). Three years later the visible extent of the cuttings had reduced, reaching 60 m from the well. Within this area the megafaunal density (0.05 individuals m−2) was lower than pre-drill and reference transects (0.23 individuals m−2). There was a significant increase in total megafaunal invertebrate densities with both distance from drilling and time since drilling although no significant interaction. Beyond the visible disturbance there were similar megafaunal densities (0.14 individuals m−2) to pre-drilling and background surveys. Species richness, Shannon-Weiner diversity and multivariate techniques showed similar patterns to density. At this site the effects of exploratory drilling on megafaunal invertebrate density and diversity seem confined to the extent of the visible cuttings pile. However, elevated Barium concentration and reduced sediment grain size suggest persistence of disturbance for three years, with unclear consequences for other components of the benthic fauna. PMID:23056177

  12. Deep-sea soundings

    NASA Astrophysics Data System (ADS)

    Brooks, David A.

    Bathymetric charts for many areas of the ocean are cheap and accurate, and we usually take their availability for granted. In these times of abundant information, it is easy to forget the wonder and excitement of the last century, when mechanical sounding machines revealed for the first time the major features of the ocean depths. Who would not be awed by the graceful sweep of the Blake Plateau or the plunging depths of the Puerto Rico Trench, and who could remain unimpressed by undersea mountain ranges more majestic than any in view? In his 1888 book, entitled Three Cruises of the Blake, Alexander Agassiz has this to say about the spectacular Caribbean bottom topography: “Compared to such panoramas the finest views of the range of the Alps sink into insignificance; it is only when we get a view of portions of the Andes from the sea-coast…that we get anything approximating to it in grandeur.”

  13. Deep sea cores available

    NASA Astrophysics Data System (ADS)

    Scientists aboard the Glomar Challenger collected a 235-m core of marine sediment specifically for geochemical study. This core, obtained with the hydraulic piston corer from site 532 (leg 75) in the South Atlantic, was frozen immediately upon its retrieval to preserve its organic geochemical properties. Samples from this core are now available to researchers.Site 532 is a reoccupation of deep-sea drilling (DSDP) site 362 of leg 40. The organic carbon content in this bioturbated core ranges between 1 and 6% and appears to fluctuate markedly on a time scale of 20,000-50,000 years. The lowest values occur in deeper sediments, and they generally are higher in younger sediments, reflecting an intensification of upwelling conditions at this location. An organic carbon maximum in upper Pliocene sediments records stronger upwelling conditions during that time.

  14. Fire in the Sea

    NASA Astrophysics Data System (ADS)

    Friedrich, Walter L.

    2000-05-01

    The legend of the lost city of Atlantis has captivated the human imagination for centuries. Did this city actually exist, and, if so, what happened to it? Was it destroyed in the greatest cataclysmic event of the Bronze Age? While the truth behind the legend of Atlantis may never be known, Fire in the Sea tells the story of one of the largest and most devastating natural disasters of classical history that may also hold vital clues to the possible existence and fate of the lost city. In vivid prose, author Walter L. Friedrich describes the eruption of the Greek island of Santorini, or Thera, sometime in the 17th or 16th century BC. This eruption, perhaps one of the largest explosions ever witnessed by humankind, sent a giant cloud of volcanic ash into the air that eventually covered settlements on the island. Friedrich relates how this event forever altered the course of civilization in the region, and inspired a mystery that has fired humanity's imagination ever since. More than 160 elegant, full-color photographs and vivid prose capture the beauty, the geology, archaeology, history, peoples and environmental setting of Santorini. Fire in the Sea will readily appeal to the general reader interested in natural catastrophies as well as the beauty of the region. It will also enchant anyone who has ever dreamt about uncovering the mystery of the legend of Atlantis. Walter Friedrich is currently an associate professor at the Department of Earth Sciences, University of Aarhus, Denmark. He has visited Santorini at least 35 times since 1975 and has published numerous scientific articles in such international journals as Nature, Lethaia, Spektrum der Wissenschaft, and other publications.

  15. Arctic Sea Ice Predictability and the Sea Ice Prediction Network

    NASA Astrophysics Data System (ADS)

    Wiggins, H. V.; Stroeve, J. C.

    2014-12-01

    Drastic reductions in Arctic sea ice cover have increased the demand for Arctic sea ice predictions by a range of stakeholders, including local communities, resource managers, industry and the public. The science of sea-ice prediction has been challenged to keep up with these developments. Efforts such as the SEARCH Sea Ice Outlook (SIO; http://www.arcus.org/sipn/sea-ice-outlook) and the Sea Ice for Walrus Outlook have provided a forum for the international sea-ice prediction and observing community to explore and compare different approaches. The SIO, originally organized by the Study of Environmental Change (SEARCH), is now managed by the new Sea Ice Prediction Network (SIPN), which is building a collaborative network of scientists and stakeholders to improve arctic sea ice prediction. The SIO synthesizes predictions from a variety of methods, including heuristic and from a statistical and/or dynamical model. In a recent study, SIO data from 2008 to 2013 were analyzed. The analysis revealed that in some years the predictions were very successful, in other years they were not. Years that were anomalous compared to the long-term trend have proven more difficult to predict, regardless of which method was employed. This year, in response to feedback from users and contributors to the SIO, several enhancements have been made to the SIO reports. One is to encourage contributors to provide spatial probability maps of sea ice cover in September and the first day each location becomes ice-free; these are an example of subseasonal to seasonal, local-scale predictions. Another enhancement is a separate analysis of the modeling contributions. In the June 2014 SIO report, 10 of 28 outlooks were produced from models that explicitly simulate sea ice from dynamic-thermodynamic sea ice models. Half of the models included fully-coupled (atmosphere, ice, and ocean) models that additionally employ data assimilation. Both of these subsets (models and coupled models with data assimilation) have a far narrower spread in their prediction, indicating that the results of these more sophisticated methods are converging. Here we summarize and synthesize the 2014 contributions to the SIO, highlight the important questions and challenges that remain to be addressed, and present data on stakeholder uses of the SIO and related SIPN products.

  16. Contrasts in Arctic shelf sea-ice regimes and some implications: Beaufort Sea versus Laptev Sea

    USGS Publications Warehouse

    Reimnitz, E.; Dethleff, D.; Nurnberg, D.

    1994-01-01

    The winter ice-regime of the 500 km) from the mainland than in the Beaufort Sea. As a result, the annual freeze-up does not incorporate old, deep-draft ice, and with a lack of compression, such deep-draft ice is not generated in situ, as on the Beaufort Sea shelf. The Laptev Sea has as much as 1000 km of fetch at the end of summer, when freezing storms move in and large (6 m) waves can form. Also, for the first three winter months, the polynya lies inshore at a water depth of only 10 m. Turbulence and freezing are excellent conditions for sediment entrainment by frazil and anchor ice, when compared to conditions in the short-fetched Beaufort Sea. We expect entrainment to occur yearly. Different from the intensely ice-gouged Beaufort Sea shelf, hydraulic bedforms probably dominate in the Laptev Sea. Corresponding with the large volume of ice produced, more dense water is generated in the Laptev Sea, possibly accompanied by downslope sediment transport. Thermohaline convection at the midshelf polynya, together with the reduced rate of bottom disruption by ice keels, may enhance benthic productivity and permit establishment of open-shelf benthic communities which in the Beaufort Sea can thrive only in the protection of barrier islands. Indirect evidence for high benthic productivity is found in the presence of walrus, who also require year-round open water. By contrast, lack of a suitable environment restricts walrus from the Beaufort Sea, although over 700 km farther to the south. We could speculate on other consequences of the different ice regimes in the Beaufort and Laptev Seas, but these few examples serve to point out the dangers of exptrapolating from knowledge gained in the North American Arctic to other shallow Arctic shelf settings. ?? 1994.

  17. Polarimetric backscattering from sea ice

    NASA Technical Reports Server (NTRS)

    Nghiem, S. V.; Kwok, R.; Yueh, S. H.

    1993-01-01

    Polarimetric backscattering from sea ice is presented. Theoretical models for backscattering are first presented for various ice types. Then, theoretical results are compared with experimental data for new thin ice, first-year ice, and multi-year ice. Sea ice is modeled as a layer medium containing random scatterers and rough interfaces. For multi-year sea ice with snow cover, the sea ice layer is modeled as an ice background with embedded spheroidal air bubbles and the snow layer as air with ice grains. The hummocky topography on multi-year ice is characterized by a Gaussian distribution which has an averaging effect on backscattering coefficients. First-year sea ice is described by an ice medium hosting ellipsoidal brine inclusions. These inclusions are oriented preferentially in the vertical direction due to the columnar structure of first-year sea ice. Azimuthally, the orientation of the brine pockets are random corresponding to the random c-axes in sea ice, unless the axes are oriented by sea currents. For thin ice in newly opened leads, it has been observed that there exists a thin brine layer with very high salinity on the top surface of the new ice. This brine layer is depicted as a medium with high permittivity which can significantly affect electromagnetic scattering signatures from the lower thin ice layer, with a higher fractional volume of brine inclusions due to higher salinity as compared to thick first-year sea ice. The rough medium interfaces are described as Gaussian rough surfaces characterized by root-mean-square heights and surface correlation lengths. The contribution from rough surface, calculated under the Kirchhoff approximation or small perturbation method, is assumed to be independent from volume scattering. The total loss including absorption and scattering losses in the scattering media is represented by the imaginary part of effective permittivity obtained from the strong fluctuation theory. The polarimetric scattering coefficients for different ice types are then derived under the distorted Born approximation.

  18. Sea Star Succumbing to Sea Star Wasting Disease

    USGS Multimedia Gallery

    Unlike their smiling cartoon brethren on television, since 2013, real-life sea stars have been suffering from a wasting disease epidemic in which they lose limbs and literally disintegrate in a matter of days. ...

  19. Tortuosity of the Antarctic Sea Ice over the Weddell Sea

    NASA Astrophysics Data System (ADS)

    Beard, J.; Heinrichs, J. F.

    2011-12-01

    The objective of the research was to mathematically characterize the edge of the Antarctic sea ice in the Weddell Sea. Because the sea ice may reflect processes involved in the atmosphere and ocean near the ice edge, it may suggest broader changes on the ice. The chosen method was to compare the tortuosity of the edge over time and across seasons. Because the sea ice may reflect processes involved in the atmosphere and ocean near the ice edge, it may suggest broader changes on the ice. Throughout the research, the shapefiles for the Antarctic sea ice were collected from the National Snow and Ice Data Center website and the coordinates were extracted using an add-in for the MapWindow GIS. These points were then put into Excel separated by year and then the distance factor (an approximation to the tortuosity) was calculated and compared by month over time. Preliminary data has shown that the closer to the winter months, the higher the tortuosity. Statistical analysis has shown that there is no clear relationship between tortuosity and the area of the sea ice, and the tortuosity exhibits a weak negative trend over the past 32 years.

  20. A Can of Sea Worms

    ERIC Educational Resources Information Center

    Zinn, Donald J.

    1977-01-01

    A comprehensive discussion of the free-living worms that inhabit the beaches and subtidal bottoms of the Cape Cod shoreline is presented. Methods for the location, collection, preservation, and identification of sea worms are identified. (BT)

  1. Doppler characteristics of sea clutter.

    SciTech Connect

    Raynal, Ann Marie; Doerry, Armin Walter

    2010-06-01

    Doppler radars can distinguish targets from clutter if the target's velocity along the radar line of sight is beyond that of the clutter. Some targets of interest may have a Doppler shift similar to that of clutter. The nature of sea clutter is different in the clutter and exo-clutter regions. This behavior requires special consideration regarding where a radar can expect to find sea-clutter returns in Doppler space and what detection algorithms are most appropriate to help mitigate false alarms and increase probability of detection of a target. This paper studies the existing state-of-the-art in the understanding of Doppler characteristics of sea clutter and scattering from the ocean to better understand the design and performance choices of a radar in differentiating targets from clutter under prevailing sea conditions.

  2. 50 CFR 648.11 - At-sea sea sampler/observer coverage.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Atlantic deep-sea red crab; or a moratorium permit for summer flounder; to carry a NMFS-certified fisheries... Atlantic deep-sea red crab permit, a skate permit, or a tilefish permit, if requested by the sea sampler... flounder, scup, black sea bass, bluefish, spiny dogfish, Atlantic herring, Atlantic deep-sea red...

  3. North Sea platforms revamped

    SciTech Connect

    O'Hare, J.

    1999-12-01

    Many of the early North Sea platforms are reaching their end-of-field life. Most are still equipped with their original drilling package. In a few cases the package has either been removed or decommissioned. The early installations were designed for much simpler and less demanding wells than the horizontal, extended-reach or designer wells common today. Extended-reach wells now can be drilled realistically from ageing platforms, without incurring massive capital expenditure. This can be achieved using the existing drilling package to the limit of its capabilities and supplementing where necessary with relatively minor upgrades or the use of temporary equipment. Drilling even a few more wells from existing platforms not only prolongs field life, it enables any surplus processing capacity to be made available to develop near-field potential with extended-reach drilling (ERD) or by tying back subsea satellite wells, or for processing third-party fluids. The paper describes well design, surface equipment, mud pumps, shakers and solids control equipment, drill cuttings disposal systems, derrick and hoisting system, top drive and drillstring, downhole equipment, well planning, casing wear, logistics, rig preparations, and ERD vs. subsea tie-backs.

  4. Exploring the deeper seas

    SciTech Connect

    Witt, R.R., Jr.

    1980-06-05

    The history, current status, and economics of offshore exploration for crude oil and natural gas in water depths over 600 ft since it became a reality in 1965 are reviewed. Development has not progressed as originally anticipated, and only three deepwater areas - the Gulf of Mexico, California, and the North Sea - account for 54% of all wells drilled but have less than 10% of the world's prospective deepwater acreage. Deepwater drilling has barely begun - only one deepwater well has been drilled per 17 million prospective deepwater acres. To date expenditures for deepwater drilling have been uneconomical; however, the tension leg platform is seen as possibly an economical means of producing hydrocarbons from deepwater areas. The enormous front-end expenditure has greatly limited the development of deepwater production. Only the largest oil companies can enter into the risky and very capital-intensive ventures, and even the largest companies can only afford to develop the largest fields which offer the best potential for return of capital. Increased oil prices and stable or good economic conditions of the major industralized nations will probably impact the volume of petroleum consumed in such a way as to render development of deepwater areas economical in the future. (BLM)

  5. Applied Sea Ice Research

    NASA Astrophysics Data System (ADS)

    Løset, S.

    2009-04-01

    In the late 1960s oil and gas development became an issue in the northern coastal areas of Alaska and Canada. More lately this has also become an issue in the Euroasian Arctic with the Barents and Kara Seas as example on where offshore hydrocarbon production now is being planned. In such waters the key questions prior to a development are related to water depths at the site and in case of ice, how frequent and what type of ice features will be met. Especially the ice conditions and knowledge about them are very decisive for the field development solutions to be chosen. The paper will highlight examples on development solutions where the ice conditions have played a paramount role in the field development plans. An example is the consequences of iceberg threaten in an area and the effect sudden changes in ice drift directions may have on the exploration and drilling solutions chosen. The paper will also discuss how to derive design ice actions values for such waters including scaling from nature to model ice basins.

  6. A Sea of Lava

    NASA Technical Reports Server (NTRS)

    2003-01-01

    [figure removed for brevity, see original site]

    Islands of older high-standing terrain rise above a sea of relatively young, platy lava flows between two of the largest volcanoes in the solar system.

    Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time.

    NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the California Institute of Technology in Pasadena.

    Image information: VIS instrument. Latitude 10.6, Longitude 158.5 East (201.5 West). 19 meter/pixel resolution.

  7. Arctic Sea Ice Model Sensitivities

    NASA Astrophysics Data System (ADS)

    Peterson, K. J.; Bochev, P.; Paskaleva, B.

    2010-12-01

    Arctic sea ice is an important component of the global climate system and, due to feedback effects, the Arctic ice cover is changing rapidly. Predictive mathematical models are of paramount importance for accurate estimates of the future ice trajectory. However, the sea ice components of Global Climate Models (GCMs) vary significantly in their prediction of the future state of Arctic sea ice and have generally underestimated the rate of decline in minimum sea ice extent seen over the past thirty years. One of the contributing factors to this variability is the sensitivity of the sea ice state to internal model parameters. A new sea ice model that holds some promise for improving sea ice predictions incorporates an anisotropic elastic-decohesive rheology and dynamics solved using the material-point method (MPM), which combines Lagrangian particles for advection with a background grid for gradient computations. We evaluate the variability of this MPM sea ice code and compare it with the Los Alamos National Laboratory CICE code for a single year simulation of the Arctic basin using consistent ocean and atmospheric forcing. Sensitivities of ice volume, ice area, ice extent, root mean square (RMS) ice speed, central Arctic ice thickness,and central Arctic ice speed with respect to ten different dynamic and thermodynamic parameters are evaluated both individually and in combination using the Design Analysis Kit for Optimization and Terascale Applications (DAKOTA). We find similar responses for the two codes and some interesting seasonal variability in the strength of the parameters on the solution. Sandia National Laboratories is a multi-program laboratory operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U. S. Department of Energy’s National Nuclear Security Administration under Contract DE-AC04-94-AL85000.

  8. DESERVE - Dead Sea Research Venue

    NASA Astrophysics Data System (ADS)

    Mohsen, A.; Weber, M. H.; Kottmeier, C.

    2013-12-01

    DESERVE 'Dead Sea Research Venue' focuses on the Dead Sea region as it is a unique environment and may be considered as one of the most inspiring natural laboratories on Earth. The Dead Sea Region is an exceptional ecosystem whose seismic activity has influenced all facets of the development, from ground water availability to human evolution. DESERVE addresses three grand challenges: Environmental Risks, Water Availability, Climate Change and comprises long term monitoring of geophysical parameters, studies of coupled processes in the atmosphere, hydrosphere and lithosphere as well as modeling of prediction and remediation strategies of geogenic risks. The Dead Sea has been selected for this integrated approach because it constitutes an outstanding 'natural laboratory' to study these phenomena, as - all 3 challenges are critical in this region. - the region is especially sensitive to climate change and human influences such as ground and surface water over-exploitation for agriculture and industrial purposes. - environmental processes are subject to boundary conditions that cannot be found elsewhere on Earth - understanding their interactions and the future evolution of the whole Dead Sea region are of key importance for economic development in peaceful cooperation. Results obtained in the Dead Sea region are also of prototype relevance for other (semi)-arid terminal basins of the world.

  9. Past and present Aral Sea

    NASA Astrophysics Data System (ADS)

    Dukhovniy, Viktor; Stulina, Galina; Eshchanov, Odylbek

    2013-04-01

    The tragedy of disappearing of Aral Sea is well known to the World. Before and after collapse of Soviet Union, a huge quantity of scientific and popular editions described with grief the situation around the Aral Sea. After the NIS states became independent, World Bank, UNDP, UNEP in proper competition with each other had provided some assessment of the situation through presentation of some small and medium grants, but after 2000, the local population remained alone with own problems. Although on the eyes of the present generation a unique transformation of great water body into deserts took place, the global scientific community did not find forces and financing for real and detail investigation of the processes accompanying the Sea shrinking and land formation. We should acknowledge and give big respect to NATO, later to German Government that through GTZ (now GIZ) - German International Collaboration Agency - and GFZ (Potzdam) paid attention to this area of environment crisis and organized scientific and protective design in the so-called Priaralie - the territory around the drying Sea and delta of the two rivers - Amudarya and Syrdarya. Thank to this assistance, the local specialists in collaboration with limited a number of foreign scientists (N.Aladin, P.Zavialov, Joop de Schutter, Hans Wilps, Hedi Oberhansli) organized significant works for detail socioeconomic, ecological and hydrological assessment situation in Priaralie and on the Aral sea coast. On this base, Ministry of Agriculture and Water resources of Uzbekistan and State Committee of Water resources of Kazakhstan developed a plan of rehabilitation of Amudarya and Syrdarya deltas and started implementation of these projects. If Kazakh water authority moved ahead in wetland restoration faster, a forestation of delta and drying bed of Aral Sea got big success in Uzbek territory. 244 thousands hectares of saxsaul and tamarix were planted for protection of the Priaralie. By request of GTZ SIC, ICWC organized in 2005-2009 sixth expeditions for complex remote sensing and ground investigations Aral Sea former bottom that were complemented in 2010 -2011 by two expeditions with GFZ. As a result, the landscape, soils and environment mapping was done with determination of ecologically unstable zones and assessment total change of lands situation compared with the pre-independence time. Moreover - methodic of monitoring water, environment and hydro geological indicators on the all deltas area was elaborated, organized its testing and combined with remote sensing data on Amudarya delta for 2009-2012. It permits to SIC ICWC to organize systematic permanent (decadal) monitoring and recording of size, volume and level of water in Aral Sea. Since the beginning of regular observations over the Aral Sea level, 2 periods can be emphasized: 1. Conditionally natural period - 1911-1960 - characterized by a relatively stable hydrological regime, with fluctuations in the level around 53 m and the range of inter-annual fluctuations at no more than 1 m., when the sea received annually about a half of the run-off in the Syrdarya and Amudarya Rivers, i.e. 50-60 km3/yr. 2. Intensive anthropogenic impact period - since the 1960s, a vast extension of irrigable land was carried out in Central Asia that resulted in intensive diversion of river run-off. Since then, the sea level has been falling steadily, causing a dramatic reduction in the water surface area, a decrease in water volume and depths, great changes in shoreline configuration and an expansion of the desert areas adjacent to the Aral Sea. From 1960-1985, when the sea was an integral water body, slight lowering in the sea level took place until the 1970s, when the sea-level decreased with the mean level lowering 1 m. The desiccation process accelerated visibly from the mid 1970s. In 1975-1980, the level decreased by 0.65 m a year on average. Moreover, the level dropped greatly, when the run-off of the Amudarya did not reach the Aral Sea any more (1980-1990). Kokaral was the first of the large islands becoming a peninsula, separating the Small Aral Sea in the north-east by joining the shoreline in the west. By 1986, the peninsula practically detached the small Aral Sea from the large Aral Sea, leaving only a narrow flow passage in the east. Since that time, the hydrological regimes of the Small and Large Seas have become separated. The construction of Kokaral dam in Kazakhstan, 12 km long and 8 m high, then completely separated the small Aral Sea from the large Aral Sea and changed the hydrological regimes of the water bodies. Level of this part of Sea became from this moment permanently higher than in the large Aral Sea on 42 m a.s.l. The eastern part of the sea, where the bed is much shallower and the slope is gentler is more subjected to shrinking then the western part. 2005 year became threshold, from which Eastern Aral Sea began new story - deviation from almost empty water body to almost 4 meters depth. Present assessment of water balance of Aral Sea and delta at whole dependent from delivery water river and drainage flow through control section of Samanbay on the Amudarya and some cross sections on the enter main collectors to the delta boundary. These hydrological characteristics accepted on the base of information from BWO Amudarya and our monitoring of allocation of different waters on the delta. Water volume and water surface area of Eastern and Western Aral Sea bowls were definite on the result RS data from Landsat. Bathymetric curves gave ability to assess dynamic levels of Seas. After series of enough water years 2002 - 2005 with average water income to south Priaralie 12.5 km3 period of water scarce years lead to sharp decrease of surface water area of the Eastern bowl from 1010,5 th.ha on average on two time with failure of level from 31,1 m up to average 28,5 m. But phase of permanent reducing all indicators water body changed in 2008 on deviation in range from 26.3 m to 29.5 m. Some time sharp changes in the level of water in 2.0 m take place in time one year. These changes same as degree of deltas' watering depends fully from inflow water to boundary of deltas. At the same time, the Western bowl remained more or less stable and without direct flow of surface water supported own water stability based on balance between evaporation and precipitation plus presumably the inflow of deep ground water.

  10. Magnetohydrodynamic sea water propulsion

    SciTech Connect

    Petrick, M.; Thomas, A.; Genens, L.; Libera, J.; Nietert, R.; Bouillard, J.; Pierson, E.; Hill, D.; Picologlou, B.; Ohlsson, O.; Kasprzyk, T.; Berry, G.

    1991-12-31

    An experimental and theoretical investigation of a large scale MHD propulsor has been undertaken whose objectives are to (1) investigate the transient and steady state performance of the thruster over operating parameter ranges that are compatible with achievement of high efficiency, (2) to quantify the principal loss mechanisms within the thruster and (3) to obtain preliminary hydroacoustic data. The performance of the thruster was first investigated theoretically with a 3-D code to quantify the loss mechanisms and identify experimental parameter ranges of interest. The loss mechanisms of interest are ohmic losses within the channel and those resulting from electrical currents at the entrance and exit of the thruster, and enhanced frictional losses. The analysis indicated that the relative importance of the loss mechanisms was a function of the thruster design and operating parameters. The experimental investigation of the large scale propulsor is being conducted on a sea water test facility that was designed to match the capabilities of a large 6-T superconducting magnet. The facility design was such that {approximately}90{degrees} of all losses occurred within the propulsion test train (inlet nozzle, propulsor and diffuser) thus facilitating isolation of the loss mechanisms. The test thruster itself is heavily instrumented to provide local measurements of velocity, pressure, and electric fields. The predicted overall thruster performance and value of the loss mechanisms will be compared with measured values. Comparisons will also be presented of the voltage gradients between electrodes, overall thruster efficiency, axial pressure gradients across the propulsor, change in velocity profiles, axial and vertical current distributions and exit distribution of the electrolytic gases.

  11. Magnetohydrodynamic sea water propulsion

    SciTech Connect

    Petrick, M.; Thomas, A.; Genens, L.; Libera, J.; Nietert, R.; Bouillard, J.; Pierson, E.; Hill, D.; Picologlou, B.; Ohlsson, O.; Kasprzyk, T.; Berry, G.

    1991-01-01

    An experimental and theoretical investigation of a large scale MHD propulsor has been undertaken whose objectives are to (1) investigate the transient and steady state performance of the thruster over operating parameter ranges that are compatible with achievement of high efficiency, (2) to quantify the principal loss mechanisms within the thruster and (3) to obtain preliminary hydroacoustic data. The performance of the thruster was first investigated theoretically with a 3-D code to quantify the loss mechanisms and identify experimental parameter ranges of interest. The loss mechanisms of interest are ohmic losses within the channel and those resulting from electrical currents at the entrance and exit of the thruster, and enhanced frictional losses. The analysis indicated that the relative importance of the loss mechanisms was a function of the thruster design and operating parameters. The experimental investigation of the large scale propulsor is being conducted on a sea water test facility that was designed to match the capabilities of a large 6-T superconducting magnet. The facility design was such that {approximately}90{degrees} of all losses occurred within the propulsion test train (inlet nozzle, propulsor and diffuser) thus facilitating isolation of the loss mechanisms. The test thruster itself is heavily instrumented to provide local measurements of velocity, pressure, and electric fields. The predicted overall thruster performance and value of the loss mechanisms will be compared with measured values. Comparisons will also be presented of the voltage gradients between electrodes, overall thruster efficiency, axial pressure gradients across the propulsor, change in velocity profiles, axial and vertical current distributions and exit distribution of the electrolytic gases.

  12. SeaWinds - Greenland

    NASA Technical Reports Server (NTRS)

    1999-01-01

    The frequent coverage provided by NASA's SeaWinds instrument on the QuikScat satellite provides unprecedented capability to monitor daily and seasonal changes in the key melt zones of Greenland, which is covered with a thick ice sheet that resulted from snow accumulating over tens of thousands of years. The thickness of the snow layers reveals details about the past global climate, and comparing snow accumulation and snow melting can provide insight into climate change and global warming. In particular, the extent of summer melting of snow in Greenland is considered a sensitive indicator of global change.

    Earlier scatterometer data has suggested that Greenland has experienced significantly more melting in recent years. This figure compares the melting observed over 15 days during July 1999 in Greenland. The red areas around the central blue and white areas are the main melt zones and have lower radar back scatter because of water on the surface that saturates the surface snow. As the days warm up, the melt extent dramatically increases. Comparing this data with computer models and past scatterometer data will help scientists evaluate the inter-annual variability of the melting as a step toward understanding potential climate change.

    The world's large ice sheets in Greenland and Antarctica act as vast storehouses of freshwater. Summer season melting releases large quantities of freshwater into the ocean, and year-to-year variations can have a significant impact on global sea level. Furthermore, long-term changes in the patterns and extent of melting on the large ice sheets reflect the effects of climate variability; thus Greenland is considered a sensitive indicator of global warming.

    Satellite microwave radars are extremely sensitive to melting and can provide the only effective means of accurately measuring the year-round picture of the extent and variability in ice sheet melting. Daily mean images were produced from QuikScat data collected over the Greenland ice sheet at the height of the present summer melt period. In the top row, four images are shown at intervals of 5 days, for (a) day 203, (b) 208,(c) 213, and (d) 218 in 1999. Blue and white colors indicate surfaces which are cold and dry, while read and black indicate wet snow surfaces experiencing melting. The coastal regions are lower in elevation and begin to melt first. As summer progresses, the area of melting expands inland and northwards along the western coast of Greenland as air temperatures warm. A large pale and dark blue region in the central, high-elevation part of the ice sheet survives each summer without experiencing any melting. This is known as the dry snow region, and its area is a measure of the stability of the central part of the ice sheet. The line dividing the melt area and the dry snow is very sensitive to climate conditions and monitoring this line will help scientists determine whether the Earth's climate is changing.

    The lower series of four images shows the daily variability in the radar data within each image. White patches in these images identify regions where the most rapid changes are taking place. Air temperature and precipitation variations are responsible for the patterns, with the greatest impact over the southern tip of Greenland occurring from storms originating over the Atlantic. Note that the red areas of central and northern Greenland experience much smaller or slower changes, with the central ice sheet showing little change during this summer period.

    With its frequent coverage, the SeaWinds instrument is a power and unique tool for monitoring the health of the large ice sheets. The continuing time-series of data is a valuable contribution to assessments of the effects and impact of global change in the polar regions.

    NASA's Earth Science Enterprise is a long-term research and technology program designed to examine Earth's land, oceans, atmosphere, ice and life as a total integrated system. JPL is a division of the California Institute of Technology, Pasadena, CA.

  13. SeaWinds - Greenland

    NASA Technical Reports Server (NTRS)

    1999-01-01

    The frequent coverage provided by NASA's SeaWinds instrument on the QuikScat satellite provides unprecedented capability to monitor daily and seasonal changes in the key melt zones of Greenland, which is covered with a thick ice sheet that resulted from snow accumulating over tens of thousands of years. The thickness of the snow layers reveals details about the past global climate, and comparing snow accumulation and snow melting can provide insight into climate change and global warming. In particular, the extent of summer melting of snow in Greenland is considered a sensitive indicator of global change.

    Earlier scatterometer data has suggested that Greenland has experienced significantly more melting in recent years. This figure compares the melting observed over 15 days during July 1999 in Greenland. The red areas around the central blue and white areas are the main melt zones and have lower radar back scatter because of water on the surface that saturates the surface snow. As the days warm up, the melt extent dramatically increases. Comparing this data with computer models and past scatterometer data will help scientists evaluate the inter-annual variability of the melting as a step toward understanding potential climate change.

    The world's large ice sheets in Greenland and Antarctica act as vast storehouses of freshwater. Summer season melting releases large quantities of freshwater into the ocean, and year-to-year variations can have a significant impact on global sea level. Furthermore, long-term changes in the patterns and extent of melting on the large ice sheets reflect the effects of climate variability; thus Greenland is considered a sensitive indicator of global warming.

    Satellite microwave radars are extremely sensitive to melting and can provide the only effective means of accurately measuring the year-round picture of the extent and variability in ice sheet melting. Daily mean images were produced from QuikScat data collected over the Greenland ice sheet at the height of the present summer melt period. In the top row, four images are shown at intervals of 5 days, for (a) day 203, (b) 208,(c) 213, and (d) 218 in 1999. Blue and white colors indicate surfaces which are cold and dry, while read and black indicate wet snow surfaces experiencing melting. The coastal regions are lower in elevation and begin to melt first. As summer progresses, the area of melting expands inland and northwards along the western coast of Greenland as air temperatures warm. A large pale and dark blue region in the central, high-elevation part of the ice sheet survives each summer without experiencing any melting. This is known as the dry snow region, and its area is a measure of the stability of the central part of the ice sheet. The line dividing the melt area and the dry snow is very sensitive to climate conditions and monitoring this line will help scientists determine whether the Earth's climate is changing.

    The lower series of four images shows the daily variability in the radar data within each image. White patches in these images identify regions where the most rapid changes are taking place. Air temperature and precipitation variations are responsible for the patterns, with the greatest impact over the southern tip of Greenland occurring from storms originating over the Atlantic. Note that the red areas of central and northern Greenland experience much smaller or slower changes, with the central ice sheet showing little change during this summer period.

    With its frequent coverage, the SeaWinds instrument is a power and unique tool for monitoring the health of the large ice sheets. The continuing time-series of data is a valuable contribution to assessments of the effects and impact of global change in the polar regions.

    NASA's Earth Science Enterprise is a long-term research and technology program designed to examine Earth's land, oceans, atmosphere, ice and life as a total integrated system. JPL is a division of the California Institute of Technology, Pasadena

  14. Deep-sea Hexactinellida (Porifera) of the Weddell Sea

    NASA Astrophysics Data System (ADS)

    Janussen, Dorte; Tabachnick, Konstantin R.; Tendal, Ole S.

    2004-07-01

    New Hexactinellida from the deep Weddel Sea are described. This moderately diverse hexactinellid fauna includes 14 species belonging to 12 genera, of which five species and one subgenus are new to science: Periphragella antarctica n. sp., Holascus pseudostellatus n. sp., Caulophacus (Caulophacus) discohexactinus n. sp., C. ( Caulodiscus) brandti n. sp., C. ( Oxydiscus) weddelli n. sp., and C. ( Oxydiscus) n. subgen. So far, 20 hexactinellid species have been reported from the deep Weddell Sea, 15 are known from the northern part and 10 only from here, while 10 came from the southern area, and five of these only from there. However, this apparent high "endemism" of Antarctic hexactinellid sponges is most likely the result of severe undersampling of the deep-sea fauna. We find no reason to believe that a division between an oceanic and a more continental group of species exists. The current poor database indicates that a substantial part of the deep hexactinellid fauna of the Weddell Sea is shared with other deep-sea regions, but it does not indicate a special biogeographic relationship with any other ocean.

  15. Cenozoic stratigraphic evolution, North Sea and Labrador Sea

    SciTech Connect

    Gradstein, F.M.; Grant, A.C.; Mudford, B.S. ); Berggren, W.A. ); Kaminski, M.A. ); D'Lorio, M.A. ); Cloetingh, S. ); Griffiths, C.M. )

    1990-05-01

    The authors are studying Cenozoic correlation patterns, burial trends, and subsidence history of the Central North Sea, Labrador, and Orphan basins. The authors objectives are (1) to detail intraregional mid-high latitude biozonations using noise filtering and probabilistic zonation techniques; (2) to detail paleobathymetric trends from basin margins to centers; (3) to apply this knowledge to model basin evolution, in the perspective of the evolving North Atlantic Ocean; (4) to evaluate causes for the occurrence of major hiatuses and rapid changes of subsidence; and (5) to relate rapid changes in sedimentation in the last few millions of years to model observed undercompaction trends. Cenozoic microfossil assemblages in these basins are similar, related to similarities in sedimentary and paleoeceanographic conditions. In more basinal wells, flysch-type agglutinated foraminiferal assemblages occur, also known from Carpathians, Trinidad, and Moroccan foredeeps. Over 90% of agglutinated taxa are common between these basins, although local stratigraphic ranges vary sufficiently to rely on the concept of average ranges, rather than total ones for correlations. Cenozoic stratigraphic resolution in the North Sea and Labrador basins generally is in 3-5-Ma units. and paleobathymetric zonations define a minimum of five niches, from inner shelf to middle slope regimes. Significant hiatuses occurred in the late Eocene through the Miocene, particularly in northern Labrador and northern North Sea. Subsidence in the Labrador/Grand Banks passive margin half grabens was strongly influenced by Labrador Sea opening between anomalies 34 (Campanian) and 13 (early Oligocene), when subsidence exceeded sedimentation and bathyal conditions prevailed along the margin. Thermally induced subsidence in the central North Sea grabens was considerable in the late Paleocene, when the Norwegian Sea started to open.

  16. Sea Ice Concentration and Extent

    NASA Technical Reports Server (NTRS)

    Comiso, Josefino C.

    2014-01-01

    Among the most seasonal and most dynamic parameters on the surface of the Earth is sea ice which at any one time covers about 3-6% of the planet. In the Northern Hemisphere, sea ice grows in extent from about 6 x 10(exp 6) sq km to 16 x 10(exp 6) sq km, while in the Southern Hemisphere, it grows from about 3 x 10(exp 6) sq km to about 19 x 10(exp 6) sq km (Comiso, 2010; Gloersen et al., 1992). Sea ice is up to about 2-3 m thick in the Northern Hemisphere and about 1 m thick in the Southern Hemisphere (Wadhams, 2002), and compared to the average ocean depth of about 3 km, it is a relatively thin, fragile sheet that can break due to waves and winds or melt due to upwelling of warm water. Being constantly advected by winds, waves, and currents, sea ice is very dynamic and usually follows the directions of the many gyres in the polar regions. Despite its vast expanse, the sea ice cover was previously left largely unstudied and it was only in recent years that we have understood its true impact and significance as related to the Earths climate, the oceans, and marine life.

  17. Satellite views of the Bohai Sea, Yellow Sea, and East China Sea

    NASA Astrophysics Data System (ADS)

    Shi, Wei; Wang, Menghua

    2012-10-01

    A comprehensive study of water properties for the Bohai Sea (BS), Yellow Sea (YS), and East China Sea (ECS) has been carried out with 8-year observations between 2002 and 2009 from the Moderate Resolution Imaging Spectroradiometer (MODIS) onboard the Aqua platform. Normalized water-leaving radiance spectra (nLw(?)), chlorophyll-a concentration (Chl-a), diffuse attenuation coefficient at the wavelength of 490 nm (Kd(490)), total suspended matter (TSM), and sea surface temperature (SST) are used to quantify and characterize the physical, optical, biological, and biogeochemical properties and their seasonal and interannual variability in the BS, YS, and ECS regions. The BS, YS, and ECS feature highly turbid waters in the coastal regions and river estuaries with high Kd(490) over ?3 m-1 and TSM concentrations reach over ?50 g m-3. The optical, biological, and biogeochemical property features in these three seas show considerable seasonal variability. The dominant empirical orthogonal function (EOF) mode for Kd(490) and TSM variability in the BS, YS, and ECS regions is the seasonal mode, which accounts for about two-thirds of the total variance. Phytoplankton dynamics in open oceans of the BS, YS, and ECS is also found to play an important role in the Kd(490) variation, while its impact on the ocean turbidity (Kd(490)) is much less than that of seasonal winds and sea surface thermodynamics in coastal regions. The first EOF mode in SST for the regions is seasonal and accounts for nearly 90% of the total SST variance. The major mechanisms that drive ocean color property variations in the BS, YS, and ECS are the seasonal winds, ocean stratification, and sea surface thermodynamics due to the seasonal climate change, as well as coastal bathymetry, seasonal phytoplankton blooms, and river discharges.

  18. Global Sea Rise: a Redetermination

    NASA Astrophysics Data System (ADS)

    Douglas, Bruce C.

    It is well established that sea level trends obtained from tide gauge records shorter than about 50-60 years are corrupted by interdecadal sea level variation. However, only a fraction (<25%) of even the long records exhibit globally consistent trends, because of vertical crustal movements. The coherent trends are from tide gauges not at collisional plate boundaries, and not located in or near areas deeply ice-covered during the last glaciation. Douglas (1991), using ICE-3G values for the postglacial (PGR) rebound correction, found 21 usable records (minimum length 60 years, average 76) in 9 oceanographic groups that gave a mean trend for global sea level rise of 1.8 mm/yr ± 0.1 for the period 1880-1980. In that analysis, a significant inconsistency of PGR-corrected U.S. east coast trends was noted, but not resolved. Now, even after eliminating those trends, more (24) long records (minimum 60 years, average 83) are available, including series in the southern hemisphere not previously used. The mean trend of 9 groups made up of the newly-selected records is also 1.8 mm/yr ± 0.1 for global sea level rise over the last 100+ years. A somewhat smaller set of longer records in 8 groups (minimum 70 years, average 91) gives 1.9 mm/yr ± 0.1 for the mean trend. These values are about an order of magnitude larger than the average over the last few millennia. The recent (in historical terms) dramatic increase in the rate of global sea level rise has not been explained, and no acceleration during the last century has been detected. This situation requires additional investigation and confirmation. VLBI/GPS/absolute gravity measurements of crustal motions can be employed to correct many long (60+ years) tide gauge records not now usable because of vertical crustal movements, improving the geographic coverage of sea level trends. Direct altimetric satellite determinations of global sea level rise from satellites such as TOPEX/POSEIDON and its successors can provide an independent estimate in possibly a decade or so, and thereby ascertain whether or not there has been any recent change in the rate of global sea level rise.

  19. Internal and external forcing of sea level variability in the Black Sea

    NASA Astrophysics Data System (ADS)

    Volkov, Denis L.; Landerer, Felix W.

    2015-11-01

    The variability of sea level in the Black Sea is forced by a combination of internal and external processes of atmospheric, oceanic, and terrestrial origin. We use a combination of satellite altimetry and gravity, tide gauge, river discharge, and atmospheric re-analysis data to provide a comprehensive up-to-date analysis of sea level variability in the Black Sea and to quantify the role of different environmental factors that force the variability. The Black Sea is part of a large-scale climatic system that includes the Mediterranean and the North Atlantic. The seasonal sea level budget shows similar contributions of fresh water fluxes (precipitation, evaporation, and river discharge) and the Black Sea outflow, while the impact of the net surface heat flux is smaller although not negligible. We find that the nonseasonal sea level time series in the Black and Aegean seas are significantly correlated, the latter leading by 1 month. This lag is attributed to the adjustment of sea level in the Black Sea to externally forced changes of sea level in the Aegean Sea and to the impact of river discharge. The nonseasonal sea level budget in the Black Sea is dominated by precipitation and evaporation over the sea itself, but external processes such as river discharge and changes in the outflow can also cause some large synoptic-scale sea level anomalies. Sea level is strongly coupled to terrestrial water storage over the Black Sea drainage basin, which is modulated by the North Atlantic Oscillation (NAO). We show that during the low/high NAO southwesterly/northeasterly winds near the Strait of Gibraltar and southerly/northerly winds over the Aegean Sea are able to dynamically increase/decrease sea level in the Mediterranean and Black seas, respectively.

  20. Orientation and open-sea navigation in sea turtles

    PubMed

    Lohmann; Lohmann

    1996-01-01

    Loggerhead sea turtle hatchlings (Caretta caretta L.) emerge from underground nests, scramble to the sea and begin a transoceanic migration by swimming away from their natal beach and into the open ocean. Evidence suggests that hatchlings sequentially use three different sets of cues to maintain orientation during their initial migration offshore. While on the beach, hatchlings find the ocean by crawling towards the lower, brighter seaward horizon and away from the dark, elevated silhouettes of vegetation and dunes. Upon entering the ocean, turtles initially orient seawards by swimming into waves, which can be detected as orbital movements from under water. Laboratory experiments have demonstrated that turtles can transfer a course initiated on the basis of waves or visual cues to a course mediated by a magnetic compass. Thus, by setting a magnetic course on the basis of nearshore cues that indicate the seaward direction, hatchlings may continue on offshore headings after entering deep water beyond sight of land. Sea turtles may use the earth's magnetic field not only as a cue for compass orientation but also as a source of world-wide positional information. Recent experiments have demonstrated that loggerheads can detect subtle differences in magnetic field inclination and intensity, two geomagnetic features that vary across the surface of the earth. Because most nesting beaches and oceanic regions are marked by a unique combination of these features, these findings raise the possibility that adult sea turtles navigate using a bicoordinate magnetic map. PMID:9317364

  1. Future sea-level rise in the Mediterranean Sea

    NASA Astrophysics Data System (ADS)

    Galassi, Gaia; Spada, Giorgio

    2014-05-01

    Secular sea level variations in the Mediterranean Sea are the result of a number of processes characterized by distinct time scales and spatial patterns. Here we predict the future sea level variations in the Mediterranean Sea to year 2050 combining the contributions from terrestrial ice melt (TIM), glacial isostatic adjustment (GIA), and the ocean response (OR) that includes the thermal expansion and the ocean circulation contributions. The three contributions are characterized by comparable magnitudes but distinctly different sea-level fingerprints across the Mediterranean basin. The TIM component of future sea-level rise is taken from Spada et al. (2013) and it is mainly driven by the melt of small glaciers and ice caps and by the dynamic ice loss from Antarctica. The sea-level fingerprint associated with GIA is studied using two distinct models available from the literature: ICE-5G(VM2) (Peltier, 2004) and the ice model progressively developed at the Research School of Earth Sciences (RSES) of the National Australian University (KL05) (see Fleming and Lambeck, 2004 and references therein). Both the GIA and the TIM sea-level predictions have been obtained with the aid of the SELEN program (Spada and Stocchi, 2007). The spatially-averaged OR component, which includes thermosteric and halosteric sea-level variations, recently obtained using a regional coupled ocean-atmosphere model (Carillo et al., 2012), vary between 2 and 7 cm according to scenarios adopted (EA1B and EA1B2, see Meehl at al., 2007). Since the sea-level variations associated with TIM mainly result from the gravitational interactions between the cryosphere components, the oceans and the solid Earth, and long-wavelength rotational variations, they are characterized by a very smooth global pattern and by a marked zonal symmetry reflecting the dipole geometry of the ice sources. Since the Mediterranean Sea is located in the intermediate far-field of major ice sources, TIM sea-level changes have sub-eustatic values (i.e. they do not exceed the global average) and show little (but still significant) variations across the basin associated with the subsidence driven by the meltwater load. For year 2050, TIM calculations predict a sea-level rise of ~10 and ~30 cm for the mid range and the high end scenarios, respectively. Mainly because of the distinct mantle viscosity profiles adopted in ICE-5G(VM2) and KL05, the GIA patterns differ significantly and, in contrast with the TIM fingerprint, are both characterized by strong variations across the Mediterranean Sea, showing maximum values in the bulk of the basin. For the OR component, a significant geographical variation is observed across the Mediterranean sub-basins, corresponding to different Atlantic boundary conditionsAccording to this study, the total future sea-level rise for year 2050 will reach maximum values for the extreme scenario (hig-hend prediction for TIM, KL05 for GIA and EA1B2 for OR) of ˜ 27 cm in average with peak of ˜ 30 cm in the central sub-basins. Our results show that when these three components of future sea-level rise are simultaneously considered, the spatial variability is enhanced because of the neatly distinct geometry of the three fingerprints. References: Carillo, A., Sannino, G., Artale, V., Ruti, P., Calmanti, S., DellAquila, A., 2012, Clim. Dyn. 39 (9-10), 2167-2184; Fleming, K. and Lambeck, K., 2004, Quat. Sci. Rev. 23 (9-10), 1053-1077; Meehl, G.A., and 11 others, 2007, in Climate Change 2007: The Physical Science Basis, Cambridge University Press; Peltier W.R., 2004, Annu. Rev. Earth Pl. Sc., 32, 111-149; Spada, G. and Stocchi, P., 2007, Comput. and Geosci., 33(4), 538-562; Spada G., Bamber J. L., Hurkmans R. T. W. L., 2013, Geophys. Res. Lett., 1-5, 40.

  2. Factors controlling ebro deep-sea fan growth, Mediterranean Sea

    SciTech Connect

    Nelson, C.H.; Maldonado, A.; Alonso, B.; Palanques, A.; Ryan, W.B.F.; Kastens, K.; O'Connel, S.

    1985-01-01

    Tectonic, sediment-source and sea-level factors control depositional patterns of the Ebro deep-sea fan system. In unstable, steep continental slope terrain, mass movement of material results in wide gullied canyons and formation of non-channelized debris aprons. These fan channels develop low sinuosity and generally traverse the continental rise without feeding into depositional lobes because of steep gradients (1:50 to 1:100) and sediment draining into the subsiding Valencia Valley graben. An abundance of sediment input points from mass failure and many river-fed canyons contributes to a depositional pattern of side-by-side debris aprons and separate channel-levee complexes. When a large sediment supply feeds a channel for a relatively long period 1) fan valley sinuosity increases: 2) channel walls are modified through undercutting, slumping, and crevasse splays: 3) channel bifurcation occurs: 4) incipient depositional lobe formation begins. Lowering of sea levels in Late Pleistocene time permitted the access of coarse river sediment to slope valleys and promoted deposition of numerous turbidites and active growth of the fan. During the Holocene, when sea levels have been high, a regime of hemipelagic sedimentation, mass movement, and debris apron sedimentation has dominated.

  3. Sea level annual cycle in sea level from tide gauges, altimetry and ocean models in the Polar Seas

    NASA Astrophysics Data System (ADS)

    Cheng, Y.; Andersen, O. B.

    2012-04-01

    Lack of adequate spatial and temporal sea level observations in the Polar Seas is one of the most challenging problems in the study of sea level variation and ocean circulation in the regions. The tide gauge data captures local sea level variability with long time span of data. Most of the stations are situated at the Norwegian and Russian border and hereby only observing the easternmost part of the Polar Seas.On the other hand, obtaining satellite data in high latitude regions is generally very problematic. In the Polar Seas, sea level has a significant annual cycle over the coastal regions. In this work, Simple Ocean Data Assimilation (SODA, 1950-2008) and DRAKKAR (ORCA025-G70, 1958-2004) ocean reanalysis are used in conjunction with tide gauge data and altimetric data to investigate the sea level annual cycle in the Polar Seas. The preliminary results show that the amplitude and phase of sea level annual cycle from different data sources are quite difference with each other for the period 1950-1979 and 1980-2008 over the selected regions. To compare with the results from gridded altimetric sea level anomaly maps (ERS-1, ERS-2 and Envisat), more investigation is performed from 1993 to 2009. Moreover, the annual sea level variation from CryoSat-2 data is used for the cross validation.

  4. Sea-Floor Spreading and Transform Faults

    ERIC Educational Resources Information Center

    Armstrong, Ronald E.; And Others

    1978-01-01

    Presents the Crustal Evolution Education Project (CEEP) instructional module on Sea-Floor Spreading and Transform Faults. The module includes activities and materials required, procedures, summary questions, and extension ideas for teaching Sea-Floor Spreading. (SL)

  5. Sea Otter Pup Wants the Worm

    USGS Multimedia Gallery

    A sea otter pup watches eagerly as its mother eats a fat innkeeper worm in Monterey Bay, California. USGS scientists study sea otters in efforts to help the threatened species continue to recover from near extinction....

  6. Antarctic sea ice mapping using the AVHRR

    SciTech Connect

    Zibordi, G. ); Van Woert, M.L. . SeaSpace, Inc.)

    1993-08-01

    A sea ice mapping scheme based on Advanced Very High Resolution Radiometer (AVHRR) data from the National Oceanic and Atmospheric Administration (NOAA) polar orbiting satellites has been developed and applied to daylight images taken between November 1989 to January 1990 and November 1990 to January 1991 over the Weddell and the Ross Seas. After masking the continent and ice shelves, sea ice is discriminated from clouds and open sea using thresholds applied to the multidimensional space formed by AVHRR Channel 2, 3, and 4 radiances. Sea ice concentrations in cloud-free regions are then computed using the tie-point method. Results based on the analysis of more than 70 images show that the proposed scheme is capable of properly discriminating between sea ice, open sea, and clouds, under most conditions, thus allowing high resolution sea ice maps to be produced during the austral summer season.

  7. Annual sea level variability of the coastal ocean: The Baltic Sea-North Sea transition zone

    NASA Astrophysics Data System (ADS)

    Passaro, M.; Cipollini, P.; Benveniste, J.

    2015-04-01

    The annual cycle is a major contribution to the nontidal variability in sea level. Its characteristics can vary substantially even at a regional scale, particularly in an area of high variability such as the coastal ocean. This study uses previously validated coastal altimetry solutions (from ALES data set) and the reference ESA Sea Level Climate Change Initiative data set to improve the understanding of the annual cycle during the Envisat years (2002-2010) in the North Sea-Baltic Sea transition area. This area of study is chosen because of the complex coastal morphology and the availability of in situ measurements. To our knowledge, this is the first time that the improvements brought by coastal satellite altimetry to the description of the annual variability of the sea level have been evaluated and discussed. The findings are interpreted with the help of a local climatology and wind stress from a reanalysis model. The coastal amplitude of the annual cycle estimated from ALES altimetry data is in better agreement with estimations derived from in situ data than the one from the reference data set. Wind stress is found to be the main driver of annual cycle variability throughout the domain, while different steric contributions are responsible for the differences within and among the subbasins. We conclude that the ALES coastal altimetry product is a reliable data set to study the annual cycle of the sea level at a regional scale, and the strategy described in this research can be applied to other areas of the coastal ocean where the coverage from the tide gauges is not sufficient.

  8. Tides of the British Seas

    ERIC Educational Resources Information Center

    Sandon, Frank

    1975-01-01

    Examines the gravitational effects and the way that local conditions interact with these effects to produce the tides characteristic of the British seas. Presents some effects of tides including the possibility of harnessing tidal energy and the effect of tidal friction on the use of the earth as a clock. (GS)

  9. Sea Lamprey, an Invasive Fish

    USGS Multimedia Gallery

    Invasive sea lamprey prey on commercially important fish species such as lake trout, living off of the blood and body fluids of adult fish. It is one of many fish species that USGS scientists study from the USGS Research Vessel Muskie. These lamprey belong to the Great Lakes Fisheries Commissio...

  10. Sea Lamprey, an Invasive Fish

    USGS Multimedia Gallery

    Invasive sea lamprey prey on commercially important fish species such as lake trout, living off of the blood and body fluids of adult fish.  It is one of many fish species that USGS scientists study from the USGS Research Vessel Muskie. These lamprey belong to the Great Lakes Fisheries Com...

  11. Photonic aspect of sea shell

    NASA Astrophysics Data System (ADS)

    Sarkar, Aditi; Gangopadhyay, Arnab; Sarkar, A.

    2013-02-01

    Sea shells are the bio-originating layered tough shelters of mollusks. Optical aspects and the functional nature of it are studied in this work using experimental probes like Fourier transform infra-red spectroscopy (FTIR) and UVVIS spectroscopy. It shows Photonic behavior in IR region of electromagnetic wave. Corresponding photonic band gap is estimated.

  12. Salton Sea Ecosystem Monitoring Project

    USGS Publications Warehouse

    Miles, A. Keith; Ricca, Mark A.; Meckstroth, Anne; Spring, Sarah E.

    2009-01-01

    The Salton Sea is critically important for wintering and breeding waterbirds, but faces an uncertain future due to water delivery reductions imposed by the Interstate and Federal Quantification Settlement Agreement of 2003. The current preferred alternative for wetland restoration at the Salton Sea is saline habitat impoundments created to mitigate the anticipated loss of wetland habitat. In 2006, a 50-hectare experimental complex that consisted of four inter-connected, shallow water saline habitat ponds (SHP) was constructed at the southeastern shoreline of the Salton Sea and flooded with blended waters from the Alamo River and Salton Sea. The present study evaluated ecological risks and benefits of the SHP concept prior to widespread restoration actions. This study was designed to evaluate (1) baseline chemical, nutrient, and contaminant measures from physical and biological constituents, (2) aquatic invertebrate community structure and colonization patterns, and (3) productivity of and contaminant risks to nesting waterbirds at the SHP. These factors were evaluated and compared with those of nearby waterbird habitat, that is, reference sites.

  13. Energy from the sea waves

    SciTech Connect

    Blandino, A.; Brighenti, A.; Vielmo, P.

    1980-01-01

    The main results relevant to certain themes are shown and discussed together with the computerized procedures by which they have been obtained. These themes include the following: (a) Evaluation of the sea wave energy content; (b) Interaction between the incident wave field and the absorbing device; and (c) Economic assessment of the wave power generator.

  14. Westward intensification in marginal seas

    NASA Astrophysics Data System (ADS)

    Chen, Gengxin; Xue, Huijie

    2014-03-01

    An idealized model was used to examine why the strong western boundary current (WBC) is observed in the South China Sea (SCS) but not in the Gulf of Mexico (GOM) and Japan/East Sea (JES). Results suggested that the stronger WBC in the SCS is mainly attributed to the direct contribution of the inflow and the strong monsoon. Although the Gulf Stream transports a large amount of water into the GOM, the passage in the southeast corner guides the inflow out of the gulf and inhibits the inflow from intensifying the WBC. Meanwhile, the wind stress in the GOM is weakest among the three marginal seas. The meridional ocean ridge and the particular layout of the continental slope of JES prevent the whole basin from participating in the westward intensification. Besides, the throughflow has adverse effects on the formulation of WBC in JES. The variation of Coriolis parameter with latitude leads to the westward intensification in marginal seas. However, a strong WBC cannot be observed in the absence of reasonable collocation of wind, inflow, and topography.

  15. A Deep-Sea Simulation.

    ERIC Educational Resources Information Center

    Montes, Georgia E.

    1997-01-01

    Describes an activity that simulates exploration techniques used in deep-sea explorations and teaches students how this technology can be used to take a closer look inside volcanoes, inspect hazardous waste sites such as nuclear reactors, and explore other environments dangerous to humans. (DDR)

  16. The Sea Ice Board Game

    ERIC Educational Resources Information Center

    Bertram, Kathryn Berry

    2008-01-01

    The National Science Foundation-funded Arctic Climate Modeling Program (ACMP) provides "curriculum resource-based professional development" materials that combine current science information with practical classroom instruction embedded with "best practice" techniques for teaching science to diverse students. The Sea Ice Board Game, described…

  17. Sea Ice and Oceanographic Conditions.

    ERIC Educational Resources Information Center

    Oceanus, 1986

    1986-01-01

    The coastal waters of the Beaufort Sea are covered with ice three-fourths of the year. These waters (during winter) are discussed by considering: consolidation of coastal ice; under-ice water; brine circulation; biological energy; life under the ice (including kelp and larger animals); food chains; and ice break-up. (JN)

  18. Ploughing the deep sea floor.

    PubMed

    Puig, Pere; Canals, Miquel; Company, Joan B; Martín, Jacobo; Amblas, David; Lastras, Galderic; Palanques, Albert

    2012-09-13

    Bottom trawling is a non-selective commercial fishing technique whereby heavy nets and gear are pulled along the sea floor. The direct impact of this technique on fish populations and benthic communities has received much attention, but trawling can also modify the physical properties of seafloor sediments, water–sediment chemical exchanges and sediment fluxes. Most of the studies addressing the physical disturbances of trawl gear on the seabed have been undertaken in coastal and shelf environments, however, where the capacity of trawling to modify the seafloor morphology coexists with high-energy natural processes driving sediment erosion, transport and deposition. Here we show that on upper continental slopes, the reworking of the deep sea floor by trawling gradually modifies the shape of the submarine landscape over large spatial scales. We found that trawling-induced sediment displacement and removal from fishing grounds causes the morphology of the deep sea floor to become smoother over time, reducing its original complexity as shown by high-resolution seafloor relief maps. Our results suggest that in recent decades, following the industrialization of fishing fleets, bottom trawling has become an important driver of deep seascape evolution. Given the global dimension of this type of fishery, we anticipate that the morphology of the upper continental slope in many parts of the world’s oceans could be altered by intensive bottom trawling, producing comparable effects on the deep sea floor to those generated by agricultural ploughing on land. PMID:22951970

  19. The Sea Ice Board Game

    ERIC Educational Resources Information Center

    Bertram, Kathryn Berry

    2008-01-01

    The National Science Foundation-funded Arctic Climate Modeling Program (ACMP) provides "curriculum resource-based professional development" materials that combine current science information with practical classroom instruction embedded with "best practice" techniques for teaching science to diverse students. The Sea Ice Board Game, described…

  20. A “Rosetta Stone” for metazoan zooplankton: DNA barcode analysis of species diversity of the Sargasso Sea (Northwest Atlantic Ocean)

    NASA Astrophysics Data System (ADS)

    Bucklin, Ann; Ortman, Brian D.; Jennings, Robert M.; Nigro, Lisa M.; Sweetman, Christopher J.; Copley, Nancy J.; Sutton, Tracey; Wiebe, Peter H.

    2010-12-01

    Species diversity of the metazoan holozooplankton assemblage of the Sargasso Sea, Northwest Atlantic Ocean, was examined through coordinated morphological taxonomic identification of species and DNA sequencing of a ˜650 base-pair region of mitochondrial cytochrome oxidase I (mtCOI) as a DNA barcode (i.e., short sequence for species recognition and discrimination). Zooplankton collections were made from the surface to 5,000 meters during April, 2006 on the R/V R.H. Brown. Samples were examined by a ship-board team of morphological taxonomists; DNA barcoding was carried out in both ship-board and land-based DNA sequencing laboratories. DNA barcodes were determined for a total of 297 individuals of 175 holozooplankton species in four phyla, including: Cnidaria (Hydromedusae, 4 species; Siphonophora, 47); Arthropoda (Amphipoda, 10; Copepoda, 34; Decapoda, 9; Euphausiacea, 10; Mysidacea, 1; Ostracoda, 27); and Mollusca (Cephalopoda, 8; Heteropoda, 6; Pteropoda, 15); and Chaetognatha (4). Thirty species of fish (Teleostei) were also barcoded. For all seven zooplankton groups for which sufficient data were available, Kimura-2-Parameter genetic distances were significantly lower between individuals of the same species (mean=0.0114; S.D. 0.0117) than between individuals of different species within the same group (mean=0.3166; S.D. 0.0378). This difference, known as the barcode gap, ensures that mtCOI sequences are reliable characters for species identification for the oceanic holozooplankton assemblage. In addition, DNA barcodes allow recognition of new or undescribed species, reveal cryptic species within known taxa, and inform phylogeographic and population genetic studies of geographic variation. The growing database of "gold standard" DNA barcodes serves as a Rosetta Stone for marine zooplankton, providing the key for decoding species diversity by linking species names, morphology, and DNA sequence variation. In light of the pivotal position of zooplankton in ocean food webs, their usefulness as rapid responders to environmental change, and the increasing scarcity of taxonomists, the use of DNA barcodes is an important and useful approach for rapid analysis of species diversity and distribution in the pelagic community.

  1. Sea Turtle Tracks at Nesting Site

    USGS Multimedia Gallery

    A morning photo depicts evidence of a sea turtle nesting the previous night (from the sea, left; return to sea, right). The protective stakes mark a nest from an earlier week as part of a county research program that marks and records every eighth nest....

  2. Sea Grant: Enhancing K-12 Education.

    ERIC Educational Resources Information Center

    Fortner, Rosanne W.

    1998-01-01

    Sea Grant is a major contributor to marine and aquatic education in K-12 classrooms through curriculum development, teacher education, school programs at field sites, and educational research. Describes Sea Grant's efforts in these areas. Specific programs outlined include Operation Pathfinder, Ohio Sea Grant Partnerships for Great Lakes…

  3. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true SEA flexibility. 300.230 Section 300.230 Education... DISABILITIES Local Educational Agency Eligibility § 300.230 SEA flexibility. (a) Adjustment to State fiscal... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and...

  4. 33 CFR 2.22 - Territorial sea.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Territorial sea. 2.22 Section 2... Jurisdictional Terms § 2.22 Territorial sea. (a) With respect to the United States, the following apply— (1) Territorial sea means the waters, 12 nautical miles wide, adjacent to the coast of the United States...

  5. 33 CFR 2.22 - Territorial sea.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Territorial sea. 2.22 Section 2... Jurisdictional Terms § 2.22 Territorial sea. (a) With respect to the United States, the following apply— (1) Territorial sea means the waters, 12 nautical miles wide, adjacent to the coast of the United States...

  6. 33 CFR 2.22 - Territorial sea.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Territorial sea. 2.22 Section 2... Jurisdictional Terms § 2.22 Territorial sea. (a) With respect to the United States, the following apply— (1) Territorial sea means the waters, 12 nautical miles wide, adjacent to the coast of the United States...

  7. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false SEA flexibility. 300.230 Section 300.230 Education... DISABILITIES Local Educational Agency Eligibility § 300.230 SEA flexibility. (a) Adjustment to State fiscal... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and...

  8. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false SEA flexibility. 300.230 Section 300.230 Education... DISABILITIES Local Educational Agency Eligibility § 300.230 SEA flexibility. (a) Adjustment to State fiscal... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and...

  9. 33 CFR 2.22 - Territorial sea.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Territorial sea. 2.22 Section 2... Jurisdictional Terms § 2.22 Territorial sea. (a) With respect to the United States, the following apply— (1) Territorial sea means the waters, 12 nautical miles wide, adjacent to the coast of the United States...

  10. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true SEA flexibility. 300.230 Section 300.230 Education... DISABILITIES Local Educational Agency Eligibility § 300.230 SEA flexibility. (a) Adjustment to State fiscal... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and...

  11. 33 CFR 2.22 - Territorial sea.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Territorial sea. 2.22 Section 2... Jurisdictional Terms § 2.22 Territorial sea. (a) With respect to the United States, the following apply— (1) Territorial sea means the waters, 12 nautical miles wide, adjacent to the coast of the United States...

  12. Isotopic composition and origin of the red sea and salton sea geothermal brines.

    PubMed

    Craig, H

    1966-12-23

    Abstract. Deuterium and oxygen-18 measurements show that the Red Sea and Salton Sea brines are the results of a single process, the leaching of sediments by surface water circulating downward to a geothermal reservoir. The Salton Sea brine is derived from local precipitation but the Red Sea brine originates 1000 kilometers south of its basin, on the shallow sill which controls the circulation of the Red Sea. On this sill sea water penetrates a thick evaporite sequence to a depth of 2000 meters, and, driven by its increased density relative to sea water, flows northward to emerge in the brine-filled deeps. PMID:17807292

  13. The Impact of Sea Surface Temperature Front on Stratus-Sea Fog over the Yellow and East China Seas

    NASA Astrophysics Data System (ADS)

    Zhang, S.; Li, M.; Liu, F.

    2013-12-01

    A stratus-sea fog event occurred on 3 June 2011 over the Yellow and East China Seas (as shown in figure) is investigated observationally and numerically. Emphasis is put on the influences of the sea surface temperature front (SSTF) and of the synoptic circulations on the transition of stratus to sea fog. The southerly winds from a synoptic high pressure transport water vapor from the East China Sea to the Yellow Sea, while the subsidence induced by the high contributes to the formation of the temperature inversion on the top of the stratus or stratocumulus that appears mainly over the warm flank of a sea surface temperature front in the East China Sea. Forced by the SSTF, there is a secondary cell within the atmospheric boundary layer (ABL), with a sinking branch on the cold flank and a rising one on the warm flank of the SSTF. This sinking branch, in phase with the synoptic subsidence, forces the stratus or stratocumulus to lower in the elevation getting close to the sea surface as these clouds move northward driven by the southerly winds. The cloud droplets can either reach to the sea surface directly or evaporate into water vapor that may condense again when coming close to the cold sea surface to form fog. In this later case, the stratus and fog may separate. The cooling effect of cold sea surface counteracts the adiabatic heating induced by the subsidence and thus helps the transition of stratus to sea fog in the southern Yellow Sea. By smoothing the SSTF in the numerical experiment, the secondary cell weakens and the sea fog patches shrink obviously over the cold flank of the SSTF though the synoptic subsidence and moist advection still exist. A conceptual model is suggested for the transition of stratus to sea fog in the Yellow and East China Seas, which is helpful for the forecast of sea fog over these areas. The satellite visible image of the stratus-fog event. The fog appears in the Yellow Sea and the stratocumulus in the East China Sea.

  14. Facing up to sea rise.

    PubMed

    Pernetta, J

    1994-01-01

    A milder and less extreme climate, abundance of fish and mollusks, transport and communication, and fertile land at low altitudes have drawn humans to coastal areas and river valleys for centuries. More than 60% of the world's population occupy the 150 km closest to the coast. Millions of tourists come to coastlines and small tropical islands for recreation. About 80% of the global fish supply originates from the 19 km closest to the shore. Fish are the only protein source for the rapidly growing populations in many developing countries. Global climate change is increasing the sea level so much that by 2050, the mean increase will be about 38 cm (24-52 cm). The Intergovernmental Panel on Climate Change (IPCC) notes that the effects of sea level rise will differ from place to place. Direct effects are flooding of low-lying coastal areas and increased erosion rates. An indirect effect includes higher water tables and intrusion of saline water into aquifers, resulting in loss of fresh ground water resources. These effects may make coastal areas less suitable for settlement and agriculture. Coral sand settled on top of coral reefs makes up the unstable, small atoll islands of the Pacific and Indian Oceans. People on these islands depend almost entirely on the sea for their livelihood and on the small amount of fresh water with which they grow root crops and drink. Rising sea levels also threaten low lying countries, e.g., Bangladesh, and densely populated deltas, e.g., the Nile. Changes in the frequency and severity of flooding will increase Bangladesh's dependence on foreign aid. IPCC sees 3 possible responses to the rising sea level: defense, retreat, and accommodation. Accommodation is the only practical choice for many developing countries. A switch from rice cultivation to mariculture of prawns and fish is an example of accommodation. We need to more completely understand the natural processes in coastal environments to be better prepared for climate change. PMID:12287486

  15. Management of the Wadden Sea

    NASA Astrophysics Data System (ADS)

    Wolff, W. J.; Zijlstra, J. J.

    1980-03-01

    The Wadden Sea situated along the North Sea coasts of Denmark, the Federal Republic of Germany and The Netherlands represents one of the world's largest bar-built type of estuaries. The area is a typical sedimentation and mineralization basin, with a large influx of organic matter from the adjoining North Sea, consequently a delicate oxygen balance and a rich benthic macrofauna, poor in species, which serves as food for juveniles of some commercially important North Sea fishes and for large numbers of migrating and wintering waders and waterfowl. Past and present activities of the human society in the area include fisheries (mainly for shrimp and mussels, semi-culture), shipping, land reclamation, recreation, dredging for sand and shells, and waste discharge from industries and human communities. Until the present these activities, although sometimes conflicting, did not fundamentally affect the area and its biota (pollution excluded), but future claims, including the construction of large deep-sea harbours, drilling for natural gas and oil, large-scale land reclamation and increased industrialization etc., might gradually induce degradation. For instance, area reduction by continued land reclamation could lead to irreversible losses of specific biotopes (e. g. salt-marshes, mud-flats), which could affect the size of bird and fish populations in a much wider region. Increased pollution, which has already inflicted damage on bird and seal populations, could reduce the fauna and hence the value of the area as a natural sanctuary. In the event of a proposal for a new human activity in the area, the present standing practice in the countries concerned requires an evaluation of its safety and economic aspects and its environmental impact. However, the various plans are considered separately and there is a general need for integrated management of the area.

  16. Thermal biology of sea snakes and sea kraits.

    PubMed

    Heatwole, Harold; Grech, Alana; Monahan, John F; King, Susan; Marsh, Helene

    2012-08-01

    Temperature probably had no direct effect on the evolution of sea kraits within their center of origin, a geologically stable thermal zone straddling the equator, but may have indirectly affected expansions and contractions in distributions beyond that zone through global fluctuations that caused alternation of higher and lower sea levels. The northern limit of the Laticauda colubrina complex seems to be the 20°C isotherm; in the south, the range does not reach that isotherm because there is no land (also a habitat requirement of sea kraits) within the zone of suitable temperature. The relationship of temperature to the pattern of geographic variation in morphology supports either the hypothesis of peripheral convergence or the developmental hypothesis but does not distinguish between them. Quadratic surfaces relating cumulative scores for coloration and morphological characters to global position showed a strong latitudinal component and an even stronger longitudinal one in which the direction of the latitudinal effect was reversed between east and west. A multivariate analysis revealed that while morphological characters vary significantly by location and climate when tested separately, when the influence of location on morphology is taken into account, no residual relationship between climate and morphology remains. Most marine snakes have mean upper temperature tolerances between 39°C and 40°C and operate at temperatures much nearer their upper thermal limits than their lower limits but still avoid deleterious extremes by diving from excessively hot water to deeper, cooler strata, and by surfacing when water is cold. At the surface in still water in sunlight, Pelamis can maintain its body temperature slightly above that of the water, but whether this is significant in nature is questionable. As temperature falls below 18-20°C, survival time is progressively reduced, accompanied by the successive occurrence of cessation of feeding, cessation of swimming, and failure to orient. Acclimation does not seem to be in this species' repertoire. In the water column, marine snakes track water temperature; on land, sea kraits can thermoregulate by basking, selecting favorable locations, and by kleptothermy. Laticauda colubrina adjusts its reproductive cycle geographically in ways that avoid breeding in the coldest months. Mean voluntary diving time is not temperature-dependent within the normal range of temperatures experienced by marine snakes in the field, but is reduced in water colder than 20°C. On land, much as while diving in the sea, sea kraits maintain long periods of apnea; intervals between breaths are inversely related to temperature. PMID:22669175

  17. High resolution trace element records from the deep sea hydrocoral Stylaster venustus: Implications for stylasterids as a paleoceanographic archive

    NASA Astrophysics Data System (ADS)

    Aranha, R. S.; Layne, G. D.; Edinger, E.; Piercey, G.

    2009-12-01

    Stylasterids are one of the lesser known groups of deep sea corals, but appear to have potential to serve as viable geochemical archives for reconstructing temperature, salinity and nutrient regimes in the deep ocean. This group of hydrocorals are present in most, if not all of the world’s major oceans. Stylasterid species dominantly have aragonitic skeletons, with a small percentage of species having calcitic skeletons (1). A recent study on the biomineralization of a deep sea stylasterid (Errina dabneyi) has revealed that during the organism’s growth, a steady dissolution and reprecipitation of skeletal material occurs in the central canals of the skeleton. This skeletal modification likely alters the stable isotope and/or trace element profiles of these corals, making them potentially less reliable as geochemical archives, depending on the scale of sampling (2). Recent specimens of Stylaster venustus were collected in July, 2008 from the Olympic Coast National Marine sanctuary off the coast of Washington at depths of 200 - 350 m. We used a Cameca IMS 4f Secondary Ion Mass Spectrometer (SIMS) to perform high spatial resolution (<25 µm) spot analyses of Sr/Ca, Mg/Ca and Na/Ca in detailed traverses across the basal cross-sections from three of these specimens. We identified the remineralized material by remnant porous texture and/or a substantially different trace element composition. Spot analyses corresponding to the remineralized material were eliminated from the dataset. In all three specimens we observed a pronounced inverse correlation (r = -0.36) of Mg/Ca and Sr/Ca profiles throughout the length of the transects . A positive correlation (r =0.46) between Na/Ca and Mg/Ca profiles was also noted in two of the specimens analyzed. These correlations strongly imply that the coral skeleton is recording either cyclical or episodic variations in temperature, with possible overprinting from other environmental variation. The exact relationship between the visible banding in the skeletal cross-section and any cyclicity of trace element profiles is currently ambiguous. However, our analyses demonstrate that microanalytical techniques are a viable means of extracting trace element records from these corals. Further statistical analysis of the trace element transects, in combination with a variety of imaging analyses of the same samples, should help us elucidate what portion of the geochemical signal is temperature dependent and what magnitude of temperature change is actually being recorded. Correlating these trace element profiles with instrumental temperature records will help confirm that useful geochemical archives are preserved by stylasterid skeletons. References: (1) Cairns SD and Macintyre IG. 1992. Phylogenetic implications of calcium carbonate mineralogy in the Stylasteridae (Cnidaria:Hydrozoa).Palaios 7: 96-107. (2) Wisshak M, López Correa M, Zibrowius H, Jakobsen J & Freiwald. (in press). Skeletal reorganisation affects geochemical signals, exemplified in the stylasterid hydrocoral Errina dabneyi (Azores Archipelago). Marine Ecology Progress Series.

  18. Distribution characteristics of marine litter on the sea bed of the East China Sea and the South Sea of Korea

    NASA Astrophysics Data System (ADS)

    Lee, Dae-In; Cho, Hyeon-Seo; Jeong, Sun-Beom

    2006-10-01

    The types, quantities, and distribution of marine litter found on the sea bed of the East China Sea and the South Sea of Korea are surveyed. Surveys were evaluated using bottom trawl nets during 1996-2005 cruises. Mean distribution densities were high in coastal seas, especially in the South Sea of Korea offshore from Yeosu, with 109.8 kg km -2, and low in the East China Sea, with densities of 30.6 kg km -2. Fishing gear, such as pots, nets, octopus jars, and fishing lines, accounted for about 42-72% and 37-62% of litter items in the East China Sea and the South Sea of Korea, respectively, whereas the contributions of rubber, vinyl, metal, plastic, glass, wood, and clothing were below 30% mainly. Rope and drum composition fluctuated greatly, between 54% and 0%. Eel and net pots dominated the marine debris of the South Sea of Korea, and some vinyl, plastics, and fishing gear made in Korea, China, and Japan were collected in abundance in the East China Sea. Fishing gear was probably discarded into the sea, deliberately or inadvertently, by fishing operations. A comprehensive joint approach by Korea, China, and Japan is needed for the continuous monitoring of input sources, the actual conditions, and the behavior of marine litter for protection against litter pollution and fisheries resource management in this area.

  19. On the Sea, From the Sea, Of the Sea: The Physics of maritime Governance

    NASA Astrophysics Data System (ADS)

    James, Royce; Page, Eric

    2014-03-01

    The United States Coast Guard Academy Physics Section is proud to present our initial conceptions of ``On the Sea, From the Sea, Of the Sea: The Physics of Maritime Governance,'' a program funded by an APS Outreach Grant in 2013. In our classes, the Physics Section has focused on active student engagement for the past ten years. Recently, we have refined our program to make heavy use of Interactive Lecture Demonstrations (ILDs) and our own highly interactive adaptation which we call Interactive Lecture Labs (ILLs). ``On the Sea'' is a unique opportunity to investigate their use in a different learning modality from our standard college level military academic use. Multigenerational science, technology, engineering, and mathematics (STEM) projects are a prolific source of academic discourse, while learning through play has been touted as an effective learning tool. We plan to investigate group and individual participation, intragroup communication, demographics, and prior skill (or education) in comparison to outcomes in learning objectives through projects designed to educate the Coast Guard Academy and surrounding community on the physics of the Coast Guard's missions. Progress on the lab and demonstration designs, community participation, and our emerging ILL and ILD pedagogical methods, will be reported.

  20. Flavor Structure of Intrinsic Nucleon Sea

    NASA Astrophysics Data System (ADS)

    Peng, Jen-Chieh; Chang, Wen-Chen; Cheng, Hai-Yang; Liu, Keh-Fei

    2015-09-01

    The concept of intrinsic charm suggested by Brodsky et al. is extended to lighter quarks. Extraction of the intrinsic ?, d¯, and s¯ seas is obtained from an analysis of the d¯ - ?, s + s¯, and ? + d¯ - s -s¯ distributions. The connection between the intrinsic/extrinsic seas and the connected/disconnected seas in lattice QCD is also examined. It is shown that the connected and disconnected components for the ?(x) + d¯( x) sea can be separated. The striking x-dependence of the [ s( x) + s¯( x)]/[ ?(x) + d¯( x)] ratio is interpreted as an interplay between the connected and disconnected seas.

  1. Sea ice in the Baltic Sea A review

    NASA Astrophysics Data System (ADS)

    Granskog, Mats; Kaartokallio, Hermanni; Kuosa, Harri; Thomas, David N.; Vainio, Jouni

    2006-10-01

    Although the seasonal ice cover of the Baltic Sea has many similarities to its oceanic counterpart in Polar Seas and Oceans, there are many unique characteristics that mainly result from the brackish waters from which the ice is formed, resulting in low bulk salinities and porosities. In addition, due to the milder climate than Polar regions, the annual maximum ice extent is highly variable, and rain and freeze-melt cycles can occur throughout winter. Up to 35% of the sea ice mass can be composed from metamorphic snow, rather than frozen seawater, and in places snow and superimposed ice can make up to 50% of the total ice thickness. There is pronounced atmospheric deposition of inorganic nutrients and heavy metals onto the ice, and in the Bothnian Bay it is estimated that 5% of the total annual flux of nitrogen and phosphorus and 20-40% of lead and cadmium may be deposited onto the ice fields from the atmosphere. It is yet unclear whether or not the ice is simply a passive store for atmospherically deposited compounds, or if they are transformed through photochemical processes or biological accumulation before released at ice and snow melt. As in Polar sea ice, the Baltic ice can harbour rich biological assemblages, both within the ice itself, and on the peripheries of the ice at the ice/water interface. Much progress has been made in recent years to study the composition of these assemblages as well as measuring biogeochemical processes within the ice related to those in underlying waters. The high dissolved organic matter loading of Baltic waters and ice result in the ice having quite different chemical characteristics than those known from Polar Oceans. The high dissolved organic material load is also responsible in large degree to shape the optical properties of Baltic Sea ice, with high absorption of solar radiation at shorter wavelengths, a prerequisite for active photochemistry of dissolved organic matter. Land-fast ice in the Baltic also greatly alters the mixing characteristics of river waters flowing into coastal waters. River plumes extend under the ice to a much greater distance, and with greater stability than in ice-free conditions. Under-ice plumes not only alter the mixing properties of the waters, but also result in changed ice growth dynamics, and ice biological assemblages, with the underside of the ice being encased, in the extreme case, with a frozen freshwater layer. There is a pronounced gradient in ice types from more saline ice in the south to freshwater ice in the north. The former is characteristically more porous and supports more ice-associated biology than the latter. Ice conditions also vary considerably in different parts of the Baltic Sea, with ice persisting for over half a year in the northernmost part of the Baltic Sea, the Bothnian Bay. In the southern Baltic Sea, ice appears only during severe winters.

  2. Alkenone production in the East Sea/Japan Sea

    NASA Astrophysics Data System (ADS)

    Lee, Kyung Eun; Lee, Sunghye; Park, Yonggi; Lee, Ho Jin; Harada, Naomi

    2014-02-01

    To test the applicability of alkenones as a proxy for past sea surface temperature (SST) in the East Sea (Japan Sea), this study investigated the season and depth of alkenone production in the area. Surface and subsurface seawater samples were collected from the East Sea during cruises carried out by the National Fisheries Research and Development Institute of Korea in 2008-2010. Surface samples were filtered for suspended material at two-month intervals. Subsurface samples were collected at water depths of 20, 50, 70 and 100 m by CTD bottle casts at two stations, one a coastal station and the other an offshore station. The results of alkenone analysis show that the concentration of total C37 alkenones was generally high in the surface mixed layer and decreased with depth, indicating that alkenones were most likely produced in or close to the surface mixed layer. Alkenone concentration varied seasonally: high in spring to fall and significantly reduced in winter. Comparisons of alkenone-based temperatures with in situ seawater temperatures show that alkenone temperatures measured from suspended particles in the surface waters were close to in situ SST in summer but were lower in winter. During winter, when alkenone production is significantly reduced, alkenones may be suspended for relatively long times and are likely to be advected from the north by eddies from Subpolar Front meanders. In summer when new production of alkenones increases, the settling velocity of alkenones appears to increase and residence time becomes shorter than in winter, suggesting that particles are less likely to be significantly advected at that time. Importantly, at the offshore station, coretop alkenone temperature corresponds to annual-averaged SST, while at the coastal station it corresponds to summer-to-fall averaged SST.

  3. The distribution and diversity of sea cucumbers in the coral reefs of the South China Sea, Sulu Sea and Sulawesi Sea

    NASA Astrophysics Data System (ADS)

    Woo, Sau Pinn; Yasin, Zulfigar; Ismail, Siti Hasmah; Tan, Shau Hwai

    2013-11-01

    A study on the distribution and diversity of sea cucumbers in the coral reefs of the South China Sea, Sulu Sea and Sulawesi Sea was carried out in July 2009. The survey was done using wandering transect underwater with SCUBA. Twelve species of sea cucumber were found from four different families and nine genera. The most dominant family was Holothuriidae (five species), followed by Stichopodidae (three species), Synaptidae (three species) and Cucumariidae with only one species. The most dominant species found around the island was Pearsonothuria graffei, which can be found abundantly on substrate of dead corals in a wide range of depth (6-15 m). The Sulawesi Sea showed a higher diversity of sea cucumber with seven different species compared to the South China Sea with only six different species and Sulu Sea with only two species. Ordination by multidimensional scaling of Bray-Curtis similarities clustered the sampling locations to three main clusters with two outgroups. Previous studies done indicated a higher diversity of sea cucumber as compared to this study. This can be indication that the population and diversity of sea cucumbers in the reef is under threat.

  4. Winter snow cover on sea ice in the Weddell Sea

    NASA Astrophysics Data System (ADS)

    Massom, Robert A.; Drinkwater, Mark R.; Haas, Christian

    1997-01-01

    Measurements of snow thickness, temperature, salinity, density, and stratigraphy acquired during the 1992 Winter Weddell Gyre Study are presented. Results indicate that the winter snow cover on sea ice in the Weddell Sea is extremely variable. Extreme fluctuations in Antarctic synoptic conditions (air temperature, precipitation, humidity, and wind speed) occur during the austral winter. They result in unique modifications and additions to the snow layer during the aging process and act to stabilize an otherwise easily wind-redistributed shallow snow cover and develop well-packed drift features. The latter occur even over relatively undeformed areas of sea ice and have a significant localized effect on the snow thickness distribution. Significant variability in snow grain size (mean 2.73±3.12 mm) and density (0.32±0.09 g cm-3) is observed as a result of cyclical switches between high- and low-temperature gradient metamorphism. Multiple icy layers indicate multiple thaw-freeze events. One such event occurred during a 3-day station, during which the air temperature rose by 22°C in 12 hours (to approximately 0°C). This paper also examines mechanisms for flooding of the snow-ice interface, including snow loading. Even where the latter is not a factor, the layer of snow immediately above the snow-ice interface is commonly damp and saline (>10‰). Limitations in the data set are discussed, and comparisons are drawn with other experiments.

  5. Climate modulation on sea surface height in China seas

    NASA Astrophysics Data System (ADS)

    Zhang, Xiaoshuang; Wang, Xidong; Cao, Yingzhi; Zhang, Lianxin; Shao, Caixia; Sun, Chunjian; Wu, Xinrong; Fu, Hongli; Xuan, Lili

    2015-09-01

    The climate modulation on the sea surface height (SSH) in China seas is investigated using a China Ocean Reanalysis (CORA) dataset from 1958-2008. The dataset is constructed by assimilating the temperature/salinity profiles derived from the satellite altimetry data and historical observational temperature/salinity profiles. Based on the Empirical Orthogonal Function (EOF), the CORA sea surface height anomaly (SSHa) is decomposed, and the interannual and decadal variability of the first three leading modes are analyzed. On the interannual timescale, the first principal component (PC1) is significant positively correlated with the El Niño/Southern Oscillation (ENSO). On the decadal timescale, North Pacific Gyre Oscillation (NPGO) has significant negative correlation with PC1 whereas Pacific Decadal Oscillation (PDO) is in phase with PC3. Analysis shows that the decadal variability of SSH is mainly modulated by the wind stress curl variability related to the NPGO and PDO. In addition, the effect of net heat flux associated to the NPGO and PDO on SSH is also investigated, with net heat flux variability in the Luzon strait and tropic Pacific found to influence the decadal variability of SSH.

  6. Cabled ocean observatories in Sea of Oman and Arabian Sea

    NASA Astrophysics Data System (ADS)

    DiMarco, Steven F.; Wang, Zhankun; Jochens, Ann; Stoessel, Marion; Howard, Matthew K.; Belabbassi, Leila; Ingle, Stephanie; du Vall, Ken

    2012-07-01

    An ocean observatory—consisting of a real-time, cabled array in the Sea of Oman and an internally recording, autonomous mooring array recently upgraded to a cabled array in the northern Arabian Sea—celebrated more than 2500 days of continuous operation in July 2012. The observatory, which measures a range of properties, such as water current velocities, temperature, salinity, pressure, dissolved oxygen, and turbidity, is part of the Lighthouse Ocean Research Initiative (LORI) project [du Vall et al., 2011], which was designed as a pilot project and installed in 2005 in the region off Abu Bakara (Figures 1a and 1b). The initial goal of the project was to prove that an in situ, cabled ocean observatory can return high-quality scientific data on a real-time basis over longer time periods than conventional moored systems. That same year, an autonomous array was deployed off Ras al Hadd and on Murray Ridge in the Arabian Sea (Figure 1a).

  7. Air-Sea heat fluxes over the Iceland Sea

    NASA Astrophysics Data System (ADS)

    Petersen, Gudrun Nina; Harden, Ben E.; Renfrew, Ian A.

    2015-04-01

    On a monthly mean scale the Iceland Sea has been shown to be a region of local heat flux minimum while there are also indications that there may be dense water formation in the region. To investigate if the atmospheric conditions over the Iceland Sea can results in significant high heat flux events on shorter time scales data from a meteorological buoy, that was deployed in the area for about two years, is analysed. The observations are compared to ERA-Interim data, which is shown to generally perform very well for this central Iceland Sea location. Synoptic-scale weather patterns during different heat flux events are then examined. The high flux events with winds from the north are often found to be short lived and in between there are long period of low fluxes. Thus the high flux events are disguised in annual and monthly means. However, it still remains to be seen whether the heat fluxes in the region are large enough to drive the ocean convection needed to produce dense water.

  8. A sea of worms: polychaete checklist of the Adriatic Sea.

    PubMed

    Mikac, Barbara

    2015-01-01

    The checklist of polychaetes of the Adriatic Sea (Mediterranean) based on bibliographic sources published from 1840 to 2014, as well as on novel data, with 49 new records for the area, is herein presented. The Adriatic Sea polychaete fauna comprises at present of 764 species in 360 genera and 62 families. The richest family is the Syllidae, with 112 species (c.a. 15% of the all taxa). Eight families account for as much as 50% of the diversity (Syllidae, Serpulidae, Sabellidae, Phyllodocidae, Spionidae, Polynoidae, Terebellidae and Nereididae). Among the three Adriatic sectors (Northern, Central and Southern Adriatic), the Northern Adriatic is the richest one, whereas the composition of the most diverse families is very similar in all sectors. Data on endemisms (6), aliens (29) and valid species with the type locality in the Adriatic Sea (90) are also discussed. The list of all relevant papers citing each species in the Adriatic is included, allowing future detailed information retrievals for distinct purposes. Results suggest that the number of species will keep increasing in the future, as new surveys will be undertaken, so regular updates of the present list will be necessary. PMID:25947537

  9. Micromechanics of Sea Urchin Spines

    PubMed Central

    Tsafnat, Naomi; Fitz Gerald, John D.; Le, Hai N.; Stachurski, Zbigniew H.

    2012-01-01

    The endoskeletal structure of the Sea Urchin, Centrostephanus rodgersii, has numerous long spines whose known functions include locomotion, sensing, and protection against predators. These spines have a remarkable internal microstructure and are made of single-crystal calcite. A finite-element model of the spine’s unique porous structure, based on micro-computed tomography (microCT) and incorporating anisotropic material properties, was developed to study its response to mechanical loading. Simulations show that high stress concentrations occur at certain points in the spine’s architecture; brittle cracking would likely initiate in these regions. These analyses demonstrate that the organization of single-c