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1

Environmental sensing and response genes in Cnidaria: the chemical defensome in the sea anemone Nematostella vectensis  

PubMed Central

The starlet sea anemone Nematostella vectensis has been recently established as a new model system for the study of the evolution of developmental processes, as cnidaria occupy a key evolutionary position at the base of the bilateria. Cnidaria play important roles in estuarine and reef communities, but are exposed to many environmental stressors. Here I describe the genetic components of a ‘chemical defensome’ in the genome of N. vectensis, and review cnidarian molecular toxicology. Gene families that defend against chemical stressors and the transcription factors that regulate these genes have been termed a ‘chemical defensome,’ and include the cytochromes P450 and other oxidases, various conjugating enyzymes, the ATP-dependent efflux transporters, oxidative detoxification proteins, as well as various transcription factors. These genes account for about 1% (266/27200) of the predicted genes in the sea anemone genome, similar to the proportion observed in tunicates and humans, but lower than that observed in sea urchins. While there are comparable numbers of stress-response genes, the stress sensor genes appear to be reduced in N. vectensis relative to many model protostomes and deuterostomes. Cnidarian toxicology is understudied, especially given the important ecological roles of many cnidarian species. New genomic resources should stimulate the study of chemical stress sensing and response mechanisms in cnidaria, and allow us to further illuminate the evolution of chemical defense gene networks. PMID:18956243

Goldstone, J.V.

2010-01-01

2

Taxonomy and distribution of sea anemones (Cnidaria: Actiniaria, Corallimorpharia) from deep water of the northeastern Pacific  

E-print Network

and 2 corallimorpharian species in the deep sea of the northeastern Pacific, and fewer than half are known to science. I identified, using morphological characters, 14 of the largest and most abundant epibenthic deep-sea anemones including 12...

Eash-Loucks, Wendy Ellyn

2010-12-10

3

Sea anemones (Cnidaria, Anthozoa, Actiniaria) from coral reefs in the southern Gulf of Mexico  

PubMed Central

Abstract Seven sea anemone species from coral reefs in the southern Gulf of Mexico are taxonomically diagnosed and images from living specimens including external and internal features, and cnidae are provided. Furthermore, the known distribution ranges from another 10 species are extended. No species records of sea anemones have been previously published in the primary scientific literature for coral reefs in the southern Gulf of Mexico and thus, this study represents the first inventory for the local actiniarian fauna. PMID:24146599

González-Muñoz, Ricardo; Simões, Nuno; Tello-Musi, José Luis; Rodríguez, Estefanía

2013-01-01

4

Taxonomy and distribution of sea anemones (Cnidaria: Actiniaria and Corallimorpharia) from deep water of the northeastern Pacific  

E-print Network

Reserve, 505 Guana River Road, Ponte Vedra Beach, FL 32082, USA. Magnolia Press Auckland, New Zealand 3375 WENDY E. EASH-LOUCKS & DAPHNE G. FAUTINEASH-LOUCKS AND FAUTIN2 · Zootaxa 3375 © 2012 Magnolia Press Taxonomy and distribution of sea anemones... words: Biogeography, Coelenterata, Hexacorallia, Bathyal, Abyssal Introduction Animals belonging to cnidarian orders Actiniaria and Corallimorpharia (sea anemones sensu lato) in deep waters of the northeastern Pacific Ocean are poorly known. By contrast...

Eash-Loucks, Wendy E.; Fautin, Daphne G.

2012-07-04

5

Distribution of sea anemones (Cnidaria, Actiniaria) in Korea analyzed by environmental clustering  

E-print Network

, turbidity, and substratum. The Yellow Sea (YS) and East Sea (ES) are cold-water areas, seasonally influenced by the Yellow Sea Cold and Liman currents, respectively, from the north; the Korea Strait (KS) and Cheju Island

Fautin, Daphne

6

*Maractis rimicarivora*, a new genus and species of sea anemone (Cnidaria: Anthozoa: Actiniaria: Actinostolidae) from an Atlantic hydrothermal vent  

E-print Network

*Maractis rimicarivora* is a new genus and new species of medium-sized sea anemone (Actiniaria) from the TAG (Trans-Atlantic Geotraverse) hydrothermal vent fields (26°08.3'N, 44°49.6'W; 3650 m). The genus, which belongs to family Actinostolidae...

Fautin, Daphne G.; Barber, Brian R.

1999-01-01

7

The mitochondrial genome of the sea anemone Metridium senile (Cnidaria): introns, a paucity of tRNA genes, and a near-standard genetic code.  

PubMed Central

The circular, 17,443 nucleotide-pair mitochondrial (mt) DNA molecule of the sea anemone, Metridium senile (class Anthozoa, phylum Cnidaria) is presented. This molecule contains genes for 13 energy pathway proteins and two ribosomal (r) RNAs but, relative to other metazoan mtDNAs, has two unique features: only two transfer RNAs (tRNA(f-Met) and tRNA(Trp)) are encoded, and the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit 5 (ND5) genes each include a group I intron. The COI intron encodes a putative homing endonuclease, and the ND5 intron contains the molecule's ND1 and ND3 genes. Most of the unusual characteristics of other metazoan mtDNAs are not found in M. senile mtDNA: unorthodox translation initiation codons and partial translation termination codons are absent, the use of TGA to specify tryptophan is the only genetic code modification, and both encoded tRNAs have primary and secondary structures closely resembling those of standard tRNAs. Also, with regard to size and secondary structure potential, the mt-s-rRNA and mt-1-rRNA have the least deviation from Escherichia coli 16S and 23S rRNAs of all known metazoan mt-rRNAs. These observations indicate that most of the genetic variations previously reported in metazoan mtDNAs developed after Cnidaria diverged from the common ancestral line of all other Metazoa. PMID:9539427

Beagley, C T; Okimoto, R; Wolstenholme, D R

1998-01-01

8

Hidden among Sea Anemones: The First Comprehensive Phylogenetic Reconstruction of the Order Actiniaria (Cnidaria, Anthozoa, Hexacorallia) Reveals a Novel Group of Hexacorals  

PubMed Central

Sea anemones (order Actiniaria) are among the most diverse and successful members of the anthozoan subclass Hexacorallia, occupying benthic marine habitats across all depths and latitudes. Actiniaria comprises approximately 1,200 species of solitary and skeleton-less polyps and lacks any anatomical synapomorphy. Although monophyly is anticipated based on higher-level molecular phylogenies of Cnidaria, to date, monophyly has not been explicitly tested and at least some hypotheses on the diversification of Hexacorallia have suggested that actiniarians are para- or poly-phyletic. Published phylogenies have demonstrated the inadequacy of existing morphological-based classifications within Actiniaria. Superfamilial groups and most families and genera that have been rigorously studied are not monophyletic, indicating conflict with the current hierarchical classification. We test the monophyly of Actiniaria using two nuclear and three mitochondrial genes with multiple analytical methods. These analyses are the first to include representatives of all three currently-recognized suborders within Actiniaria. We do not recover Actiniaria as a monophyletic clade: the deep-sea anemone Boloceroides daphneae, previously included within the infraorder Boloceroidaria, is resolved outside of Actiniaria in several of the analyses. We erect a new genus and family for B. daphneae, and rank this taxon incerti ordinis. Based on our comprehensive phylogeny, we propose a new formal higher-level classification for Actiniaria composed of only two suborders, Anenthemonae and Enthemonae. Suborder Anenthemonae includes actiniarians with a unique arrangement of mesenteries (members of Edwardsiidae and former suborder Endocoelantheae). Suborder Enthemonae includes actiniarians with the typical arrangement of mesenteries for actiniarians (members of former suborders Protantheae, Ptychodacteae, and Nynantheae and subgroups therein). We also erect subgroups within these two newly-erected suborders. Although some relationships among these newly-defined groups are still ambiguous, morphological and molecular results are consistent enough to proceed with a new higher-level classification and to discuss the putative functional and evolutionary significance of several morphological attributes within Actiniaria. PMID:24806477

Rodríguez, Estefanía; Barbeitos, Marcos S.; Brugler, Mercer R.; Crowley, Louise M.; Grajales, Alejandro; Gusmão, Luciana; Häussermann, Verena; Reft, Abigail; Daly, Marymegan

2014-01-01

9

Sea Anemone: Investigations.  

ERIC Educational Resources Information Center

Several investigations can be undertaken with live sea anemones. A sea anemone's feeding response, fighting power, color, and symbiotic relationships to other invertebrates (such as a marine hermit crab) can be investigated in the high school classroom. Background information and laboratory procedures are provided. (Author/JN)

Hunt, John D.

1982-01-01

10

A new species of the sea anemone *Megalactis* (Cnidaria: Anthozoa: Actiniaria: Actinodendridae) from Taiwan and designation of a neotype for the type species of the genus  

E-print Network

*Megalactis comatus*, new species, from Taiwan is the third species in this genus of sea anemones with highly branched tentacles. The others are *M. hemprichii* Ehrenberg, 1834, from the Red Sea, and *M. griffithsi* Saville-Kent, 1893, from...

Ardelean, Adorian; Fautin, Daphne G.

2004-12-20

11

Stylobates birtlesi sp. n., a new species of carcinoecium-forming sea anemone (Cnidaria, Actiniaria, Actiniidae) from eastern Australia  

E-print Network

to International Code of Zoological Nomenclature Article 12.2.8 (International Commission on Zoological Nomenclature 1999), having been published before 1931, the name Stylobates aeneus is available for the anemone because it is a “description of the work..., 1903: p. 62 Isadamsia Carlgren, 1928a: p. 167 General. Because Dall (1903) had been under the impression that the carcinoecium upon which he based his description was that of a gastropod, the fi rst description of the anemone was by Carlgren (1928a...

Crowther, Andrea Louise; Fautin, Daphne G.; Wallace, Carden C.

2014-03-11

12

*Neoaiptasia morbilla* new species (Cnidaria: Actiniaria), a sea anemone symbiont of sand-dwelling gastropods on Saipan, Mariana Islands, with comments on some other such associations  

E-print Network

Very small, cryptic specimens of a new species of sea anemone attach to shells of living gastropods that burrow in subtidal sand on the shores of Saipan and Tinian, Mariana Islands. We have found members of the new species, ...

Fautin, Daphne G.; Goodwill, Roger H.

2009-01-01

13

Neoaiptasia morbilla new species (Cnidaria: Actiniaria), a sea anemone symbiont of sand-dwelling gastropods on Saipan, Mariana Islands, with comments on some other such associations  

Microsoft Academic Search

Very small, cryptic specimens of a new species of sea anemone attach to shells of living gastropods that burrow in subtidal sand on the shores of Saipan and Tinian, Mariana Islands. We have found members of the new species, which we describe as Neoaiptasia morbilla, on the shells of eight species of snails that belong to five families. We modify

DAPHNE G. FAUTIN; ROGER H. GOODWILL

14

Sea anemone exposed at low tide  

NSDL National Science Digital Library

Sea anemones are sensitive to drying out. To avoid drying out during low tide and periods of intense sunlight, the anemones roll up to keep their tentacles moist. The outer body of the anemone is thick and tolerant of heat.

Katie Hale (California State University, Fullerton; Student, Biological Sciences)

2007-01-05

15

A new genus and species of isanthid sea anemone (Cnidaria: Anthozoa: Actiniaria) from Chilean Patagonia, Anthoparactis fossii n. gen. et sp  

NASA Astrophysics Data System (ADS)

We describe a new genus and species of sea anemone from Chilean Patagonia. Anthoparactis fossii n. gen. et sp. adds another acontiate genus and species to the family Isanthidae Carlgren, 1938. Anthoparactis n. gen. differs from the other isanthid genera in having the same number of mesenteries distally and proximally, acontia with basitrichs only, and a column with verrucae distally. Anthoparactis fossii n. sp. differs from the most similar species, Isoparactis fionae Lauretta et al., 2013, in the number of cycles of mesenteries and tentacles, structures of the column, colour pattern of the oral disc, cnidae, and geographical distribution. Isanthidae now includes seven genera and 11 species.

Häussermann, Verena; Rodríguez, Estefanía

2014-09-01

16

Field Guide to Anemone Fishes and their Host Sea Anemones  

NSDL National Science Digital Library

Dr. Daphne G. Fautin of California Academy of Sciences and University of Kansas and Dr. Gerald R. Allen of Western Australian Museum wrote this fascinating field guide to anemone fishes and their sea anemone hosts. The five main chapters of this book cover classification and identification of sea anemones and anemone fishes in addition to their biologies, life histories, and the ecological interactions between them. Species-specific information includes common and scientific name, original description and color features/size, similar species, host species, and distribution. From students to clown fish enthusiasts to research scientists, offers a wealth of practical and intriguing information in this online book.

Allen, Gerald R.; Fautin, Daphne G.

1992-01-01

17

Genetic study of the extent and consequences of sexual and asexual reproduction in the deep-sea epizoic anemones Amphianthus inornata and Kadosactis commensalis (Cnidaria: Anthozoa)  

Microsoft Academic Search

The anemone Amphianthus inornata is found at bathyal depths living on colonies of the gorgonian Acanella arbuscula. Previous studies of the morphology and reproductive stage of this anemone, during different times of the year, have indicated\\u000a that it reproduces sexually on a seasonal basis. A small proportion of the study population were also reported to be undergoing\\u000a asexual reproduction by

S. K. Bronsdon; A. D. Rogers; P. A. Tyler; J. D. Gage

1997-01-01

18

Phylogenetic Relationships among Deep-Sea and Chemosynthetic Sea Anemones: Actinoscyphiidae and Actinostolidae (Actiniaria: Mesomyaria)  

PubMed Central

Sea anemones (Cnidaria, Actiniaria) are present in all marine ecosystems, including chemosynthetic environments. The high level of endemicity of sea anemones in chemosynthetic environments and the taxonomic confusion in many of the groups to which these animals belong makes their systematic relationships obscure. We use five molecular markers to explore the phylogenetic relationships of the superfamily Mesomyaria, which includes most of the species that live in chemosynthetic, deep-sea, and polar sea habitats and to test the monophyly of the recently defined clades Actinostolina and Chemosynthina. We found that sea anemones of chemosynthetic environments derive from at least two different lineages: one lineage including acontiate deep-sea taxa and the other primarily encompassing shallow-water taxa. PMID:20532040

Rodríguez, Estefanía; Daly, Marymegan

2010-01-01

19

Neurotoxin localization to ectodermal gland cells uncovers an alternative mechanism of venom delivery in sea anemones.  

PubMed

Jellyfish, hydras, corals and sea anemones (phylum Cnidaria) are known for their venomous stinging cells, nematocytes, used for prey and defence. Here we show, however, that the potent Type I neurotoxin of the sea anemone Nematostella vectensis, Nv1, is confined to ectodermal gland cells rather than nematocytes. We demonstrate massive Nv1 secretion upon encounter with a crustacean prey. Concomitant discharge of nematocysts probably pierces the prey, expediting toxin penetration. Toxin efficiency in sea water is further demonstrated by the rapid paralysis of fish or crustacean larvae upon application of recombinant Nv1 into their medium. Analysis of other anemone species reveals that in Anthopleura elegantissima, Type I neurotoxins also appear in gland cells, whereas in the common species Anemonia viridis, Type I toxins are localized to both nematocytes and ectodermal gland cells. The nematocyte-based and gland cell-based envenomation mechanisms may reflect substantial differences in the ecology and feeding habits of sea anemone species. Overall, the immunolocalization of neurotoxins to gland cells changes the common view in the literature that sea anemone neurotoxins are produced and delivered only by stinging nematocytes, and raises the possibility that this toxin-secretion mechanism is an ancestral evolutionary state of the venom delivery machinery in sea anemones. PMID:22048953

Moran, Yehu; Genikhovich, Grigory; Gordon, Dalia; Wienkoop, Stefanie; Zenkert, Claudia; Ozbek, Suat; Technau, Ulrich; Gurevitz, Michael

2012-04-01

20

Neurotoxin localization to ectodermal gland cells uncovers an alternative mechanism of venom delivery in sea anemones  

PubMed Central

Jellyfish, hydras, corals and sea anemones (phylum Cnidaria) are known for their venomous stinging cells, nematocytes, used for prey and defence. Here we show, however, that the potent Type I neurotoxin of the sea anemone Nematostella vectensis, Nv1, is confined to ectodermal gland cells rather than nematocytes. We demonstrate massive Nv1 secretion upon encounter with a crustacean prey. Concomitant discharge of nematocysts probably pierces the prey, expediting toxin penetration. Toxin efficiency in sea water is further demonstrated by the rapid paralysis of fish or crustacean larvae upon application of recombinant Nv1 into their medium. Analysis of other anemone species reveals that in Anthopleura elegantissima, Type I neurotoxins also appear in gland cells, whereas in the common species Anemonia viridis, Type I toxins are localized to both nematocytes and ectodermal gland cells. The nematocyte-based and gland cell-based envenomation mechanisms may reflect substantial differences in the ecology and feeding habits of sea anemone species. Overall, the immunolocalization of neurotoxins to gland cells changes the common view in the literature that sea anemone neurotoxins are produced and delivered only by stinging nematocytes, and raises the possibility that this toxin-secretion mechanism is an ancestral evolutionary state of the venom delivery machinery in sea anemones. PMID:22048953

Moran, Yehu; Genikhovich, Grigory; Gordon, Dalia; Wienkoop, Stefanie; Zenkert, Claudia; Özbek, Suat; Technau, Ulrich; Gurevitz, Michael

2012-01-01

21

The Assimilation and Distribution of Dissolved Organic Matter By Planulae of Sea Anemones  

Microsoft Academic Search

The free-living developmental stage of sea anemones (phylum Cnidaria) is the planula larva. In general the feeding biology of planulae is poorly known and there is only one report of the ability of planulae to assimilate dissolved organic materials (DaM, specifically amino acids) from seawater (Ben-David-Zaslow and Benayahu, 2000). We examined the ability of planula l arvae to assimilate dissolved

Slott Amy

2007-01-01

22

Sea anemones possess dynamic mitogenome structures.  

PubMed

A notable feature of hexacoral mitogenomes is the presence of complex self-catalytic group I introns. We investigated mitogenome structural variations and evolutionary mechanisms in actiniarian sea anemones based on the complete mitogenome sequence of the cold-water sea anemone species Urticina eques, Bolocera tuediae, Hormathia digitata and Metridium senile, and two isolates of the sub-tropical Aiptasia pulchella. Whole genome sequencing at 50 times coverage of B. tuediae and H. digitata indicated low mtDNA copy number of per haploid nuclear genome and presence of rare haplotypes. A group I intron inserted in ND5 was found to host essential mitochondrial protein genes in all species, and an additional truncated copy of ND5 in B. tuediae. A second group I intron (inserted in COI) that contained a homing endonuclease gene (HEG) was present in all mtDNA examined. Different variants of HEGs were observed, and included expressed elements fused in-frame with upstream exons and free-standing HEGs embedded within the intron. A notable hallmark of HEGs was a high extent of overlap with ribozyme structural elements; the U. eques HEG overlapped with the entire intron. We reconstructed the evolutionary history of the COI intron from insertion at unoccupied cognate sites, through HEG degradation, to intron loss. We also identified a novel insertion element in U. eques that contained two expressed protein-coding genes. An evolutionary analysis of the sea anemone mtDNA genes revealed higher substitution rates in the HEG and the insertion sequence as compared to the other loci, indicating relaxed selective pressures in these elements. We conclude that sea anemone mitogenomes are surprisingly dynamic in structure despite the economical organization and low sequence mutation rate. PMID:24613805

Emblem, Åse; Okkenhaug, Siri; Weiss, Emily S; Denver, Dee R; Karlsen, Bård Ove; Moum, Truls; Johansen, Steinar D

2014-06-01

23

NF-?B is required for cnidocyte development in the sea anemone Nematostella vectensis.  

PubMed

The sea anemone Nematostella vectensis (Nv) is a leading model organism for the phylum Cnidaria, which includes anemones, corals, jellyfishes and hydras. A defining trait across this phylum is the cnidocyte, an ectodermal cell type with a variety of functions including defense, prey capture and environmental sensing. Herein, we show that the Nv-NF-?B transcription factor and its inhibitor Nv-I?B are expressed in a subset of cnidocytes in the body column of juvenile and adult anemones. The size and distribution of the Nv-NF-?B-positive cnidocytes suggest that they are in a subtype known as basitrichous haplonema cnidocytes. Nv-NF-?B is primarily cytoplasmic in cnidocytes in juvenile and adult animals, but is nuclear when first detected in the 30-h post-fertilization embryo. Morpholino-mediated knockdown of Nv-NF-?B expression results in greatly reduced cnidocyte formation in the 5 day-old animal. Taken together, these results indicate that NF-?B plays a key role in the development of the phylum-specific cnidocyte cell type in Nematostella, likely by nuclear Nv-NF-?B-dependent activation of genes required for cnidocyte development. PMID:23063796

Wolenski, Francis S; Bradham, Cynthia A; Finnerty, John R; Gilmore, Thomas D

2013-01-01

24

High In Situ Repeatability of Behaviour Indicates Animal Personality in the Beadlet Anemone Actinia equina (Cnidaria)  

PubMed Central

‘Animal personality’ means that individuals differ from one another in either single behaviours or suites of related behaviours in a way that is consistent over time. It is usually assumed that such consistent individual differences in behaviour are driven by variation in how individuals respond to information about their environment, rather than by differences in external factors such as variation in microhabitat. Since behavioural variation is ubiquitous in nature we might expect ‘animal personality’ to be present in diverse taxa, including animals with relatively simple nervous systems. We investigated in situ startle responses in a sea anemone, Actinia equina, to determine whether personalities might be present in this example of an animal with a simple nervous system. We found very high levels of repeatability among individuals that were re-identified in the same locations over a three week sampling period. In a subset of the data, where we used tide-pool temperature measurements to control for a key element of variation in microhabitat, these high levels of repeatability remained. Although a range of other consistent differences in micro-habitat features could have contributed to consistent differences between the behaviour of individuals, these data suggest the presence of animal personality in A. equina. Rather than being restricted to certain groups, personality may be a general feature of animals and may be particularly pronounced in species with simple nervous systems. PMID:21755015

Briffa, Mark; Greenaway, Julie

2011-01-01

25

Effect of feeding regime and irradiance on the photophysiology of the symbiotic sea anemone Aiptasia pulchella  

Microsoft Academic Search

To determine how the animal and algal components of the symbiotic sea anemone Aiptasia pulchella respond to changes in food availability and culture irradiance, sea anemones from a single clone were maintained at four irradiance levels (320, 185, 115, and 45 µE m-2 s-1) and either starved or fed for 5 wk. Changes in protein biomass of sea anemones maintained

G. Muller-Parker

1985-01-01

26

Sea anemones (Cnidaria: Anthozoa: Actiniaria) of Moreton Bay  

E-print Network

-G59757, 5 spec., Cockle Bay, Magnetic I., NE Qld; NTM-C5550–51, South Shell I., Darwin, NT, 1988; NTM-C10072, East Point Sponge Gardens, Dar - win, NT, 1990; NTM-C11907, Nhulunbuy (Gove), NT, 1971; NTM-C12767, Darwin Harbour, NT, 1999; NTM-C14766...

Fautin, Daphne G.; Crowther, Andrea Louise; Wallace, Carden C.

2008-01-01

27

Mutualism with sea anemones triggered the adaptive radiation of clownfishes  

PubMed Central

Background Adaptive radiation is the process by which a single ancestral species diversifies into many descendants adapted to exploit a wide range of habitats. The appearance of ecological opportunities, or the colonisation or adaptation to novel ecological resources, has been documented to promote adaptive radiation in many classic examples. Mutualistic interactions allow species to access resources untapped by competitors, but evidence shows that the effect of mutualism on species diversification can greatly vary among mutualistic systems. Here, we test whether the development of obligate mutualism with sea anemones allowed the clownfishes to radiate adaptively across the Indian and western Pacific oceans reef habitats. Results We show that clownfishes morphological characters are linked with ecological niches associated with the sea anemones. This pattern is consistent with the ecological speciation hypothesis. Furthermore, the clownfishes show an increase in the rate of species diversification as well as rate of morphological evolution compared to their closest relatives without anemone mutualistic associations. Conclusions The effect of mutualism on species diversification has only been studied in a limited number of groups. We present a case of adaptive radiation where mutualistic interaction is the likely key innovation, providing new insights into the mechanisms involved in the buildup of biodiversity. Due to a lack of barriers to dispersal, ecological speciation is rare in marine environments. Particular life-history characteristics of clownfishes likely reinforced reproductive isolation between populations, allowing rapid species diversification. PMID:23122007

2012-01-01

28

Nanotoxicology using the sea anemone Nematostella vectensis: from developmental toxicity to genotoxicology.  

PubMed

Concomitant with the fast-growing advances in the synthesis and engineering of colloidal nanocrystals, an urgent evaluation of their toxicity on human beings and environment is strongly encouraged by public health organisations. Despite the in vitro approaches employed for toxicological screening of hazardous compounds, the use of simple and cost-effective living organisms may enormously contribute to solve unanswered questions related to embryotoxic and teratogenic effects of nanomaterials. Here, the sea anemone Nematostella vectensis (Cnidaria, Anthozoa) is presented as a novel model organism to profile bio/non-bio interactions and to show a comprehensive toxicological analysis performed on embryos, larvae and adults treated with fluorescent cadmium-based nanocrystals. Spanning from in vivo biodistribution to molecular investigations, different behaviours and effects depending on the composition and surface coatings are showed. Rod-shaped cadmium selenide/cadmium sulfide (CdSe/CdS) nanocrystals resulted in excellent imaging probes to track N. vectensis development with negligible adverse effects, while spherical CdTe nanocrystals severely impaired embryogenesis, resulting in aberrant phenotypes and deregulation of developmental genes, which raise severe worries for a safe use of this type of nanoparticles for human purposes and environmental contamination. PMID:23641943

Ambrosone, Alfredo; Marchesano, Valentina; Mazzarella, Veronica; Tortiglione, Claudia

2014-08-01

29

Two mitochondrial group I introns in a metazoan, the sea anemone Metridium senile: one intron contains genes for subunits 1 and 3 of NADH dehydrogenase.  

PubMed Central

Mitochondrial genes for cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit 5 (ND5) of the sea anemone Metridium senile (phylum Cnidaria) each contain a group I intron. This is in contrast to the reported absence of introns in all other metazoan mtDNAs so far examined. The ND5 intron is unusual in that it ends with A and contains two genes (ND1 and ND3) encoding additional subunits of NADH dehydrogenase. Correctly excised ND5 introns are not circularized but are precisely cleaved near their 3' ends and polyadenylylated to provide bicistronic transcripts of ND1 and ND3. COI introns, which encode a putative homing endonuclease, circularize, but in a way that retains the entire genome-encoded intron sequence (other group I introns are circularized with loss of a short segment of the intron 5' end). Introns were detected in the COI and ND5 genes of other sea anemones, but not in the COI and ND5 genes of other cnidarians. This suggests that the sea anemone mitochondrial introns may have been acquired relatively recently. Images Fig. 3 Fig. 4 Fig. 5 Fig. 6 PMID:8643626

Beagley, C T; Okada, N A; Wolstenholme, D R

1996-01-01

30

Detachment of Sea Anemones by Commensal Hermit Crabs and by Mechanical and Electrical Stimuli  

Microsoft Academic Search

THE ability of some hermit crabs to detach commensal sea anemones and place them on their shells has interested ecologists and ethologists for many years1. Anemones belonging to the genus Calliactis are perhaps best known in this respect. Excellent accounts of the behaviour of the Mediterranean hermit crab Dardanus (= Pagurus) arrosor Herbst, towards C. parasitica (Couch) have long been

D. M. Ross; L. Sutton

1968-01-01

31

Mobility of Stichodactyla Gigantea Sea Anemones and Implications for Resident False Clown Anemonefish, Amphiprion Ocellaris  

Microsoft Academic Search

For reef fishes that do not move between habitat patches following settlement, habitat selection is expected during settlement. Although false clown anemonefish, Amphiprion ocellaris, are sedentary following settlement, they are not especially discriminating during settlement, and are commonly found occupying anemones at which no apparent nest site exists. In this study I report on mobility of Stichodactyla gigantea sea anemones,

Jeremy S. Mitchell

2003-01-01

32

A sea anemone symbiotic with gastropods of eight species in the Mariana Islands  

E-print Network

Fifty-two specimens of the small sea anemone *Neoaiptasia morbilla* Fautin and Goodwill, 2009, were collected attached to shells of gastropods living in shallow subtidal sand on Saipan and Tinian, Mariana Islands, in 1988 ...

Goodwill, Roger H.; Fautin, Daphne G.; Furey, John; Daly, Marymegan

2009-01-01

33

Expansion of tandem repeats in sea anemone Nematostella vectensis proteome: A source for gene novelty?  

PubMed Central

Background The complete proteome of the starlet sea anemone, Nematostella vectensis, provides insights into gene invention dating back to the Cnidarian-Bilaterian ancestor. With the addition of the complete proteomes of Hydra magnipapillata and Monosiga brevicollis, the investigation of proteins having unique features in early metazoan life has become practical. We focused on the properties and the evolutionary trends of tandem repeat (TR) sequences in Cnidaria proteomes. Results We found that 11-16% of N. vectensis proteins contain tandem repeats. Most TRs cover 150 amino acid segments that are comprised of basic units of 5-20 amino acids. In total, the N. Vectensis proteome has about 3300 unique TR-units, but only a small fraction of them are shared with H. magnipapillata, M. brevicollis, or mammalian proteomes. The overall abundance of these TRs stands out relative to that of 14 proteomes representing the diversity among eukaryotes and within the metazoan world. TR-units are characterized by a unique composition of amino acids, with cysteine and histidine being over-represented. Structurally, most TR-segments are associated with coiled and disordered regions. Interestingly, 80% of the TR-segments can be read in more than one open reading frame. For over 100 of them, translation of the alternative frames would result in long proteins. Most domain families that are characterized as repeats in eukaryotes are found in the TR-proteomes from Nematostella and Hydra. Conclusions While most TR-proteins have originated from prediction tools and are still awaiting experimental validations, supportive evidence exists for hundreds of TR-units in Nematostella. The existence of TR-proteins in early metazoan life may have served as a robust mode for novel genes with previously overlooked structural and functional characteristics. PMID:20003297

2009-01-01

34

Continuous Drug Release by Sea Anemone Nematostella vectensis Stinging Microcapsules  

PubMed Central

Transdermal delivery is an attractive option for drug delivery. Nevertheless, the skin is a tough barrier and only a limited number of drugs can be delivered through it. The most difficult to deliver are hydrophilic drugs. The stinging mechanism of the cnidarians is a sophisticated injection system consisting of microcapsular nematocysts, which utilize built-in high osmotic pressures to inject a submicron tubule that penetrates and delivers their contents to the prey. Here we show, for the first time, that the nematocysts of the starlet sea anemone Nematostella vectensis can be isolated and incorporated into a topical formulation for continuous drug delivery. We demonstrate quantitative delivery of nicotinamide and lidocaine hydrochloride as a function of microcapsular dose or drug exposure. We also show how the released submicron tubules can be exploited as a skin penetration enhancer prior to and independently of drug application. The microcapsules are non-irritant and may offer an attractive alternative for hydrophilic transdermal drug delivery. PMID:24473172

Tal, Yossi; Ayalon, Ari; Sharaev, Agnesa; Kazir, Zoya; Brekhman, Vera; Lotan, Tamar

2014-01-01

35

Population genetic structure in brooding sea anemones ( Epiactis spp.) with contrasting reproductive modes  

Microsoft Academic Search

Effects of dispersal and mating systems on the genetic structure of populations were evaluated by comparing five sea anemones:\\u000a four Epiactis species that brood their offspring to the juvenile stage and one Anthopleura species that broadcasts gametes and has pelagic, planktotrophic larvae. The anemones were sampled at sites ranging from British\\u000a Columbia to southern California between 1988 and 1992 and

S. Edmands; D. C. Potts

1997-01-01

36

The Culture Sexual and Asexual Reproduction and Growth of the Sea Anemone Nematostella vectensis  

Microsoft Academic Search

Abstract. Nematostella vectensis, a widely distributed, burrowing sea anemone, was raised through successive sexual generations at room,temperature,in non-circulating seawater. It has separate sexes and,also reproduces,asex- ually by transverse fission. Cultures of animals,were,fed Artemia,sp. nauplii every second,day. Every eight days the culture water was changed, and the anemones were fed pieces,of Mytilus spp. tissue. This led to,regular spawning,by both sexes at

Cadet Hand; Kevin R. Uhlinger

37

Recruitment in a sea anemone population: juvenile substrate becomes adult prey.  

PubMed

Populations of the sea anemone Anthopleura xanthogrammica (Brandt) occur in tide pools and surge channels below intertidal mussel beds where they capture mussels dislodged by wave action and by sea star foraging. Dense concentrations of small juvenile anemones occur only within mussel beds and are probably the result of larval settlement or differential survival in that habitat. Areas experimentally cleared of anemones showed that recruitment was primarily by migrating juveniles and that the rate of immigration over a 2-year period was much higher in experimental removal areas near mussel beds than in those further away. Mussel beds thus function as an important juvenile habitat (refuge and nursery); juveniles later migrate downward and are then in a position to capture dislodged mussels and grow to adult size. PMID:17834588

Sebens, K P

1981-08-14

38

Cadherin-23 May Be Dynamic in Hair Bundles of the Model Sea Anemone Nematostella vectensis  

PubMed Central

Cadherin 23 (CDH23), a component of tip links in hair cells of vertebrate animals, is essential to mechanotransduction by hair cells in the inner ear. A homolog of CDH23 occurs in hair bundles of sea anemones. Anemone hair bundles are located on the tentacles where they detect the swimming movements of nearby prey. The anemone CDH23 is predicted to be a large polypeptide featuring a short exoplasmic C-terminal domain that is unique to sea anemones. Experimentally masking this domain with antibodies or mimicking this domain with free peptide rapidly disrupts mechanotransduction and morphology of anemone hair bundles. The loss of normal morphology is accompanied, or followed by a decrease in F-actin in stereocilia of the hair bundles. These effects were observed at very low concentrations of the reagents, 0.1–10 nM, and within minutes of exposure. The results presented herein suggest that: (1) the interaction between CDH23 and molecular partners on stereocilia of hair bundles is dynamic and; (2) the interaction is crucial for normal mechanotransduction and morphology of hair bundles. PMID:24465885

Tang, Pei-Ciao; Watson, Glen M.

2014-01-01

39

Effects of starvation, and light and dark on the energy metabolism of symbiotic and aposymbiotic sea anemones, Anthopleura elegantissima  

Microsoft Academic Search

Rates of oxygen and carbon-dioxide exhange were measured in symbiotic and aposymbiotic specimens of the sea anemone Anthopleura elegantissima while fed and starved under light or dark conditions. Respiratory quotients indicated that fed anemones switched from a carbohydrate to a fat catabolism when starved, with the exception that symbiotic individuals starved in the light showed a pronounced carbohydrate catabolism for

W. K. Fitt; R. L. Pardy

1981-01-01

40

Feeding behavior and acquisition of zooxanthellae by planula larvae of the sea anemone Anthopleura elegantissima  

Microsoft Academic Search

Symbiotic associations between cnidarians and photosynthetic dinoflagellates (i.e., zooxanthellae) are common in the marine environment. Many symbiotic cnidarians produce offspring that are initially nonsymbiotic. These new hosts must acquire symbiotic algae from environmental sources. We examined zooxanthella acquisition by laboratory-reared planula larvae of the temperate sea anemone Anthopleura elegantissima. Larvae ingested zooxanthellae while they were feeding. However, the signal that

J. A. Schwarz; V. M. Weis; D. C. Potts

2002-01-01

41

Sticholysins, two pore-forming toxins produced by the Caribbean Sea anemone Stichodactyla helianthus: Their interaction with membranes  

Microsoft Academic Search

Sticholysins (Sts) I and II (StI\\/II) are pore-forming toxins (PFTs) produced by the Caribbean Sea anemone Stichodactyla helianthus belonging to the actinoporin family, a unique class of eukaryotic PFTs exclusively found in sea anemones. As for the rest of the members of this family, Sts are cysteine-less proteins, with molecular weights around 20kDa, high isoelectric points (>9.5), and a preference

Carlos Álvarez; José M. Mancheño; Diana Martínez; Mayra Tejuca; Fabiola Pazos; María E. Lanio

2009-01-01

42

The cnidarian-bilaterian ancestor possessed at least 56 homeoboxes: evidence from the starlet sea anemone, Nematostella vectensis  

PubMed Central

Background Homeodomain transcription factors are key components in the developmental toolkits of animals. While this gene superclass predates the evolutionary split between animals, plants, and fungi, many homeobox genes appear unique to animals. The origin of particular homeobox genes may, therefore, be associated with the evolution of particular animal traits. Here we report the first near-complete set of homeodomains from a basal (diploblastic) animal. Results Phylogenetic analyses were performed on 130 homeodomains from the sequenced genome of the sea anemone Nematostella vectensis along with 228 homeodomains from human and 97 homeodomains from Drosophila. The Nematostella homeodomains appear to be distributed among established homeodomain classes in the following fashion: 72 ANTP class; one HNF class; four LIM class; five POU class; 33 PRD class; five SINE class; and six TALE class. For four of the Nematostella homeodomains, there is disagreement between neighbor-joining and Bayesian trees regarding their class membership. A putative Nematostella CUT class gene is also identified. Conclusion The homeodomain superclass underwent extensive radiations prior to the evolutionary split between Cnidaria and Bilateria. Fifty-six homeodomain families found in human and/or fruit fly are also found in Nematostella, though seventeen families shared by human and fly appear absent in Nematostella. Homeodomain loss is also apparent in the bilaterian taxa: eight homeodomain families shared by Drosophila and Nematostella appear absent from human (CG13424, EMXLX, HOMEOBRAIN, MSXLX, NK7, REPO, ROUGH, and UNC4), and six homeodomain families shared by human and Nematostella appear absent from fruit fly (ALX, DMBX, DUX, HNF, POU1, and VAX). PMID:16867185

Ryan, Joseph F; Burton, Patrick M; Mazza, Maureen E; Kwong, Grace K; Mullikin, James C; Finnerty, John R

2006-01-01

43

Seasonal gametogenesis of host sea anemone ( Entacmaea quadricolor) inhabiting Hong Kong waters  

NASA Astrophysics Data System (ADS)

Studying gonadal development of annual cycle can reveal the process of gametogenesis and reproductive period, and evaluate fertility and source utilization of a species. Host sea anemones are conspicuous members of tropical and subtropical reef ecosystems, but little is known about its biology including reproductive seasonality. Here we reported a one-year study on the gametogenesis and reproduction of host sea anemone ( Entacmaea quadricolor) inhabiting Hong Kong waters. E. quadricolor tissues were sampled in 12 occasions from 5 m and 15 m depths of water, respectively. Histological sectioning of the tissues showed that E. quadricolor was dioecious, and populational ratio of female to male was 1:1.6. The gonadal development was asynchronous within an annual cycle, which included proliferating, growing, maturing, spawning, and resting stages. The spawning occurred between August and October when surface seawater temperature reached the annual maximum (28°C), suggesting that temperature is an important factor modulating the gonadal development and mature of E. quadricolor.

Bi, Ying; Zhang, Bin; Zhang, Zhifeng; Qiu, Jianwen

2015-02-01

44

The Global Diversity of Sea Pens (Cnidaria: Octocorallia: Pennatulacea)  

PubMed Central

Recent advances in deep-sea exploration technology coupled with an increase in worldwide biotic surveys, biological research, and underwater photography in shallow water marine regions such as coral reefs, has allowed for a relatively rapid expansion of our knowledge in the global diversity of many groups of marine organisms. This paper is part of the PLoS ONE review collection of WoRMS (the Worldwide Register of Marine Species), on the global diversity of marine species, and treats the pennatulacean octocorals, a group of cnidarians commonly referred to as sea pens or sea feathers. This also includes sea pansies, some sea whips, and various vermiform taxa. Pennatulaceans are a morphologically diverse group with an estimated 200 or more valid species, displaying worldwide geographic and bathymetric distributions from polar seas to the equatorial tropics and from intertidal flats to over 6100 m in depth. The paper treats new discoveries and taxa new to science, and provides greater resolution in geographic and bathymetric distributions data than was previously known, as well as descriptions of life appearances in life and in situ observations at diverse depth. PMID:21829500

Williams, Gary C.

2011-01-01

45

Cellular changes associated with the acclimation of the intertidal sea anemone Actinia tenebrosa to ultraviolet radiation.  

PubMed

To assess the relative importance of long- and short-term cellular defense mechanisms in seasonally UV-R-acclimated Actinia tenebrosa (Anthozoa, Actiniidae), individuals were exposed to summer doses of PAR, UV-A, UV-B and enhanced UV-B (20%) for a period of 4 days. Mycosporine-like amino acids (MAAs) and cyclobutane pyrimidine dimer (CPD) concentrations were quantified, while oxidative damage to lipids and proteins, and the activities or levels of the antioxidant enzymes SOD, CAT, GR, GPOX and total glutathione were determined. Our results show that summer UV-R-acclimated individuals had a higher UV-R tolerance, with no significant increases in CPDs levels, than winter-acclimated sea anemones possibly due to higher MAA concentrations. Summer-acclimated individuals showed increased lipid and protein oxidation and GPOX activity only when they were exposed to UV-B at 20% above ambient UV-R levels. In contrast, winter-acclimated sea anemones showed elevated levels of oxidative damage, GPOX and SOD activities after exposure to UV-A or UV-B at ambient and elevated levels. Thus, this study indicates that long-term UV-R acclimation mechanisms such as the accumulation of MAAs could be more important than short-term increases in antioxidant defenses with respect to reducing indirect UV-R damage in intertidal sea anemones. PMID:25041232

Cubillos, Victor M; Lamare, Miles D; Peake, Barrie M; Burritt, David J

2014-01-01

46

Vertical and horizontal distribution patterns of the giant sea anemone Heteractis crispa with symbiotic anemonefish on a fringing coral reef  

Microsoft Academic Search

The distribution patterns of the leathery sea anemone, Heteractis crispa, which contains an algal endosymbiont (zooxanthellae) and anemonefish, were investigated in relation to size distribution on a shallow fringing reef (3.2 ha, 0–4 m depth) in Okinawa, Japan. Individual growth and movements were also examined. Large individuals (>1,000 cm2) inhabited reef edges up to a depth of 4 m, while small anemone (2)

Akihisa Hattori

2006-01-01

47

Short Toxin-like Proteins Abound in Cnidaria Genomes  

PubMed Central

Cnidaria is a rich phylum that includes thousands of marine species. In this study, we focused on Anthozoa and Hydrozoa that are represented by the Nematostella vectensis (Sea anemone) and Hydra magnipapillata genomes. We present a method for ranking the toxin-like candidates from complete proteomes of Cnidaria. Toxin-like functions were revealed using ClanTox, a statistical machine-learning predictor trained on ion channel inhibitors from venomous animals. Fundamental features that were emphasized in training ClanTox include cysteines and their spacing along the sequences. Among the 83,000 proteins derived from Cnidaria representatives, we found 170 candidates that fulfill the properties of toxin-like-proteins, the vast majority of which were previously unrecognized as toxins. An additional 394 short proteins exhibit characteristics of toxin-like proteins at a moderate degree of confidence. Remarkably, only 11% of the predicted toxin-like proteins were previously classified as toxins. Based on our prediction methodology and manual annotation, we inferred functions for over 400 of these proteins. Such functions include protease inhibitors, membrane pore formation, ion channel blockers and metal binding proteins. Many of the proteins belong to small families of paralogs. We conclude that the evolutionary expansion of toxin-like proteins in Cnidaria contributes to their fitness in the complex environment of the aquatic ecosystem. PMID:23202321

Tirosh, Yitshak; Linial, Itai; Askenazi, Manor; Linial, Michal

2012-01-01

48

Evidence for participation of GCS1 in fertilization of the starlet sea anemone Nematostella vectensis: implication of a common mechanism of sperm-egg fusion in plants and animals.  

PubMed

It has been reported that GCS1 (Generative Cell Specific 1) is a transmembrane protein that is exclusively expressed in sperm cells and is essential for gamete fusion in flowering plants. The GCS1 gene is present not only in angiosperms but also in unicellular organisms and animals, implying the occurrence of a common or ancestral mechanism of GCS1-mediated gamete fusion. In order to elucidate the common mechanism, we investigated the role of GCS1 in animal fertilization using a sea anemone (Cnidaria), Nematostella vectensis. Although the existence of the GCS1 gene in N. vectensis has been reported, the expression of GCS1 in sperm and the role of GCS1 in fertilization are not known. In this study, we showed that the GCS1 gene is expressed in the testis and that GCS1 protein exists in sperm by in situ hybridization and proteomic analysis, respectively. Then we made four peptide antibodies against the N-terminal extracellular region of NvGCS1. These antibodies specifically reacted to NvGCS1 among sperm proteins on the basis of Western analysis and potently inhibited fertilization in a concentration-dependent manner. These results indicate that sperm GCS1 plays a pivotal role in fertilization, most probably in sperm-egg fusion, in a starlet sea anemone, suggesting a common gamete-fusion mechanism shared by eukaryotic organisms. PMID:25111819

Ebchuqin, Eerdundagula; Yokota, Naoto; Yamada, Lixy; Yasuoka, Yuuri; Akasaka, Mari; Arakawa, Mio; Deguchi, Ryusaku; Mori, Toshiyuki; Sawada, Hitoshi

2014-09-01

49

Tiny Sea Anemone from the Lower Cambrian of China  

PubMed Central

Background Abundant fossils from the Ediacaran and Cambrian showing cnidarian grade grossly suggest that cnidarian diversification occurred earlier than that of other eumetazoans. However, fossils of possible soft-bodied polyps are scanty and modern corals are dated back only to the Middle Triassic, although molecular phylogenetic results support the idea that anthozoans represent the first major branch of the Cnidaria. Because of difficulties in taxonomic assignments owing to imperfect preservation of fossil cnidarian candidates, little is known about forms ancestral to those of living groups. Methods and Findings We have analyzed the soft-bodied polypoid microfossils Eolympia pediculata gen. et sp. nov. from the lowest Cambrian Kuanchuanpu Formation in southern China by scanning electron microscopy and computer-aided microtomography after isolating fossils from sedimentary rocks by acetic acid maceration. The fossils, about a half mm in body size, are preserved with 18 mesenteries including directives bilaterally arranged, 18 tentacles and a stalk-like pedicle. The pedicle suggests a sexual life cycle, while asexual reproduction by transverse fission also is inferred by circumferential grooves on the body column. Conclusions The features found in the present fossils fall within the morphological spectrum of modern Hexacorallia excluding Ceriantharia, and thus Eolympia pediculata could be a stem member for this group. The fossils also demonstrate that basic features characterizing modern hexacorallians such as bilateral symmetry and the reproductive system have deep roots in the Early Cambrian. PMID:20967244

Han, Jian; Kubota, Shin; Uchida, Hiro-omi; Stanley, George D.; Yao, Xiaoyong; Shu, Degan; Li, Yong; Yasui, Kinya

2010-01-01

50

Comparative effects of dissolved copper and copper oxide nanoparticle exposure to the sea anemone, Exaiptasia pallida.  

PubMed

Increasing use of metal oxide nanoparticles (NP) by various industries has resulted in substantial output of these NP into aquatic systems. At elevated concentrations, NP may interact with and potentially affect aquatic organisms. Environmental implications of increased NP use are largely unknown, particularly in marine systems. This research investigated and compared the effects of copper oxide (CuO) NP and dissolved copper, as copper chloride (CuCl2), on the sea anemone, Exaiptasia pallida. Sea anemones were collected over 21 days and tissue copper accumulation and activities of the enzymes: catalase, glutathione peroxidase, glutathione reductase, and carbonic anhydrase were quantified. The size and shape of CuO NP were observed using a ecanning electron microscope (SEM) and the presence of copper was confirmed by using Oxford energy dispersive spectroscopy systems (EDS/EDX). E. pallida accumulated copper in their tissues in a concentration- and time-dependent manner, with the animals exposed to CuCl2 accumulating higher tissue copper burdens than those exposed to CuO NP. As a consequence of increased copper exposure, as CuO NP or CuCl2, anemones increased activities of all of the antioxidant enzymes measured to some degree, and decreased the activity of carbonic anhydrase. Anemones exposed to CuO NP generally had higher anti-oxidant enzyme activities than those exposed to the same concentrations of CuCl2. This study is useful in discerning differences between CuO NP and dissolved copper exposure and the findings have implications for exposure of aquatic organisms to NP in the environment. PMID:25661886

Siddiqui, Samreen; Goddard, Russell H; Bielmyer-Fraser, Gretchen K

2015-03-01

51

Relationships between host and symbiont cell cycles in sea anemones and their symbiotic dinoflagellates.  

PubMed

The processes by which cnidarians and their algal endosymbionts achieve balanced growth and biomass could include coordination of host and symbiont cell cycles. We evaluated this theory with natural populations of sea anemones hosting symbiotic dinoflagellates, focusing on the temperate sea anemone Anthopleura elegantissima symbiotic with Symbiodinium muscatinei in Washington State, USA, and the tropical anemone Stichodactyla helianthus associating with unknown Symbiodinium spp. in Belize. By extruding symbiont-containing gastrodermal cells from the relatively large tentacles of these species and using nuclear staining and flow cytometry, we selectively analyzed cell cycle distributions of the symbionts and the host gastrodermal cells that house them. We found no indications of diel synchrony in host and symbiont G2/M phases, and we observed evidence of diel periodicity only in Symbiodinium spp. associated with S. helianthus but not in the anemone itself. Seasonally, S. muscatinei showed considerable G2/M phase variability among samples collected quarterly over an annual period, while the G2/M phase of its host varied much less. Within samples taken at different times of the year, correlations between host and symbiont G2/M phases ranged from very weakly to very strongly positive, with significant correlations in only half of the samples (two of four A. elegantissima samples and one of two S. helianthus samples). Overall, the G2/M phase relationships across species and sampling periods were positive. Thus, while we found no evidence of close cell cycle coupling, our results suggest a loose, positive relationship between cell cycle processes of the symbiotic partners. PMID:24243963

Dimond, James L; Pineda, Rea R; Ramos-Ascherl, Zullaylee; Bingham, Brian L

2013-10-01

52

*Anthopleura mariscali*, a new species of sea anemone (Cnidaria: Anthozoa: Actiniaria) from the Galápagos Islands  

E-print Network

. Sebens, K.P. & Paine, R.T. (1979) Biogeography of anthozoans along the west coast of South America: habi- tat, disturbance, and prey availability. Proceedings of the International Symposium on Marine Biogeogra- phy and Evolution in the Southern... A. nigrescens Tentacle Spirocyst (A) 4/4 56 14.0–22.0 x 1.8–3.3 10.3–28.8 x 1.8–3.8 Basitrich (B) 4/4 71 (8.9) 12.3–19.5 x (1.4) 1.7–3.1 (3.5) 12.0–14.4 x 1.2 Basitrich 15.6–24.0 x 1.2–2.4 Acrorhagus Holotrich I (H) 4/4 48 35.5–59.7 x 2.7–5.2 30...

Daly, Marymegan; Fautin, Daphne G.

2004-01-30

53

*Metridium farcimen*, the valid name of a common North Pacific sea anemone (Cnidaria: Actiniaria: Acontiaria)  

E-print Network

Three older names apply to the species described by Fautin et al. in 1990 as *Metridium giganteum*. They are *Actinia pripaus* Tilesius, 1809, *Actinia farcimen* Brandt, 1835, and *Isometridium rickettsi* Carlgren, 1949. ...

Fautin, Daphne G.; Hand, Cadet

2000-12-01

54

Biogeography of two species of Symbiodinium (Freudenthal) inhabiting the intertidal sea anemone Anthopleura elegantissima (Brandt).  

PubMed

We have analyzed the genetic profiles of dinoflagellate populations obtained from the Pacific coast sea anemone Anthopleura elegantissima (Brandt) at collection sites from Washington to California. Genetic differences within the symbiont populations of California anemones have been uncovered by restriction length polymorphism (RFLP) analysis of the small subunit (SSU) and large subunit (LSU) ribosomal RNA genes, and by denaturing gradient gel electrophoresis (DGGE) of the internal transcribed spacer region 2 (ITS 2). The existence of two Symbiodinium species is substantiated by sequence analysis of the variable regions V1, V2, and V3 of the SSUrDNA, which also establishes their phylogenetic relatedness to other members of the genus Symbiodinium. Anemones from Washington and Oregon harbor a single dinoflagellate species, for which we propose the name S. muscatinei sp. nov. At these northern locations, S. muscatinei either exists alone or co-occurs with the Chlorella-like green algal symbiont. Our results indicate that S. muscatinei co-occurs with a second dinoflagellate, S. californium, in mixed populations in central and southern California. We suggest that the geographic distribution of these dinoflagellates is related to the temperature cline created by latitude. PMID:11081711

Lajeunesse, T C; Trench, R K

2000-10-01

55

Abundance and diversity of anemonefishes and their host sea anemones at two mesophotic sites on the Great Barrier Reef, Australia  

NASA Astrophysics Data System (ADS)

Anemonefishes and their host sea anemones are iconic inhabitants of coral reef ecosystems. While studies have documented their abundance in shallow-water reef habitats in parts of the Indo-Pacific, none have examined these species on mesophotic reefs. In this study, we used autonomous underwater vehicle imagery to examine the abundance and diversity of anemones and anemonefishes at Viper Reef and Hydrographers Passage in the central Great Barrier Reef at depths between 50 and 65 m. A total of 37 host sea anemones (31 Entacmaea quadricolor and 6 Heteractis crispa) and 24 anemonefishes (23 Amphiprion akindynos and 1 A. perideraion) were observed. Densities were highest at Viper Reef, with 8.48 E. quadricolor and A. akindynos per 100 m2 of reef substratum. These results support the hypothesis that mesophotic reefs have many species common to shallow-water coral reefs and that many taxa may occur at depths greater than currently recognised.

Bridge, T.; Scott, A.; Steinberg, D.

2012-12-01

56

*Stylobates*: A shell-forming sea anemone (Coelenterata, Anthozoa, Actiniidae)  

E-print Network

Anatomy and cnidae distinguish two species of deep-sea actinians that produce coiled, chitinous shells inhabited by hermit crabs of the genus *Parapagurus*. The actinian type species, *Stylobates aeneus*, first assigned to the Mollusca, occurs...

Fautin, Daphne G.; Devaney, Dennis M.; Roth, Barry

1980-10-01

57

Asexual Propagation of Sea Anemones That Host Anemonefishes: Implications for the Marine Ornamental Aquarium Trade and Restocking Programs  

PubMed Central

Anemonefishes and their host sea anemones form an iconic symbiotic association in reef environments, and are highly sought after in the marine aquarium trade. This study examines asexual propagation as a method for culturing a geographically widespread and commonly traded species of host sea anemone, Entacmaea quadricolor. Two experiments were done: the first to establish whether size or colour morph influenced survival after cutting into halves or quarters; and the second to see whether feeding was needed to maximise survival and growth after cutting. Survival rates were high in both experiments, with 89.3 and 93.8% of the anemones cut in half, and 62.5 and 80.4% cut in quarters surviving in experiments 1 and 2, respectively. Anemones that were cut in half were larger in size, and healed and grew quicker than those cut in quarters. However, even though survival was lower when the individuals were cut in quarters, this treatment produced the greatest number of anemones. Feeding increased oral disc diameter growth and reduced wet weight loss, but did not significantly influence pedal disc diameter. Given that the anemones took up to 56 d to form an off-centre mouth, it is highly likely that feeding may have produced greater effect if the experiment was run for longer. This low technology method of propagation could be used to produce individuals throughout the year and the anemones could then be used to supply the aquarium trade or restock depleted habitats, thus supporting biodiversity conservation in coral reef areas. PMID:25314131

Scott, Anna; Hardefeldt, Jannah M.; Hall, Karina C.

2014-01-01

58

Introduction to Cnidaria  

NSDL National Science Digital Library

In depth site covers Cnidarian life history, ecology, morphology, systematics, and fossil record. Organisms described include corals, anemones, sea pens, box jellies, siphonophores, hydroids, fire corals, medusae, and true jellyfish. Nice explanations, diagrams, and photos.

59

Molecular characterization of two CuZn-superoxide dismutases in a sea anemone.  

PubMed

Cnidarians living in symbiosis with photosynthetic cells--called zooxanthellae--are submitted to high oxygen levels generated by photosynthesis. To cope with this hyperoxic state, symbiotic cnidarians present a high diversity of superoxide dismutases (SOD) isoforms. To understand better the mechanism of resistance of cnidarian hosts to hyperoxia, we studied copper- and zinc-containing SOD (CuZnSOD) from Anemonia viridis, a temperate symbiotic sea anemone. We cloned two CuZnSOD genes that we call AvCuZnSODa and AvCuZnSODb. Their molecular analysis suggests that the AvCuZnSODa transcript encodes an extracellular form of CuZnSOD, whereas the AvCuZnSODb transcript encodes an intracellular form. Using in situ hybridization, we showed that both AvCuZnSODa and AvCuZnSODb transcripts are expressed in the endodermal and ectodermal cells of the sea anemone, but not in the zooxanthellae. The genomic flanking sequences of AvCuZnSODa and AvCuZnSODb revealed different putative binding sites for transcription factors, suggesting different modes of regulation for the two genes. This study represents a first step in the understanding of the molecular mechanisms of host animal resistance to permanent hyperoxia status resulting from the photosynthetic symbiosis. Moreover, AvCuZnSODa and AvCuZnSODb are the first SODs cloned from a diploblastic animal, contributing to the evolutionary understanding of SODs. PMID:15451057

Plantivaux, Amandine; Furla, Paola; Zoccola, Didier; Garello, Ginette; Forcioli, Didier; Richier, Sophie; Merle, Pierre-Laurent; Tambutté, Eric; Tambutté, Sylvie; Allemand, Denis

2004-10-15

60

Fusion and retrotransposition events in the evolution of the sea anemone Anemonia viridis neurotoxin genes.  

PubMed

Sea anemones are sessile predators that use a variety of toxins to paralyze prey and foe. Among these toxins, Types I, II and III are short peptides that affect voltage-gated sodium channels. Anemonia viridis is the only sea anemone species that produces both Types I and III neurotoxin. Although the two toxin types are unrelated in sequence and three-dimensional structure, cloning and comparative analysis of their loci revealed a highly similar sequence at the 5' region, which encodes a signal peptide. This similarity was likely generated by gene fusion and could be advantageous in transcript stability and intracellular trafficking and secretion. In addition, these analyses identified the processed pseudogenes of the two gene families in the genome of A. viridis, probably resulting from retrotransposition events. As presence of processed pseudogenes in the genome requires transcription in germ-line cells, we analyzed oocyte-rich ovaries and found that indeed they contain Types I and III transcripts. This result raises questions regarding the role of toxin transcripts in these tissues. Overall, the retrotransposition and gene fusion events suggest that the genes of both Types I and III neurotoxins evolved in a similar fashion and share a partial common ancestry. PMID:19609479

Moran, Yehu; Weinberger, Hagar; Lazarus, Nimrod; Gur, Maya; Kahn, Roy; Gordon, Dalia; Gurevitz, Michael

2009-08-01

61

Catalase characterization and implication in bleaching of a symbiotic sea anemone.  

PubMed

Symbiotic cnidarians are marine invertebrates harboring photosynthesizing microalgae (named zooxanthellae), which produce great amounts of oxygen and free radicals upon illumination. Studying antioxidative balance is then crucial to understanding how symbiotic cnidarians cope with ROS production. In particular, it is suspected that oxidative stress triggers cnidarian bleaching, i.e., the expulsion of zooxanthellae from the animal host, responsible for symbiotic cnidarian mass mortality worldwide. This study therefore investigates catalase antioxidant enzymes and their role in bleaching of the temperate symbiotic sea anemone Anemonia viridis. Using specific separation of animal tissues (ectoderm and endoderm) from the symbionts (zooxanthellae), spectrophotometric assays and native PAGE revealed both tissue-specific and activity pattern distribution of two catalase electrophoretypes, E1 and E2. E1, expressed in all three tissues, presents high sensitivity to the catalase inhibitor aminotriazole (ATZ) and elevated temperatures. The ectodermal E1 form is responsible for 67% of total catalase activity. The E2 form, expressed only within zooxanthellae and their host endodermal cells, displays low sensitivity to ATZ and relative thermostability. We further cloned an ectodermal catalase, which shares 68% identity with mammalian monofunctional catalases. Last, 6 days of exposure of whole sea anemones to ATZ (0.5 mM) led to effective catalase inhibition and initiated symbiont expulsion. This demonstrates the crucial role of this enzyme in cnidarian bleaching, a phenomenon responsible for worldwide climate-change-induced mass mortalities, with catastrophic consequences for marine biodiversity. PMID:17189829

Merle, Pierre-Laurent; Sabourault, Cécile; Richier, Sophie; Allemand, Denis; Furla, Paola

2007-01-15

62

Bacterial aggregates in the tentacles of the sea anemone Metridium senile  

NASA Astrophysics Data System (ADS)

This paper provides first information on organ-like bacterial aggregates in the tentacles of the sea anemone Metridium senile. The specimens were collected from waters near Helgoland (German Bight, North Sea) and the Orkney Islands. Tentacles were prepared for morphological inspection by light and scanning electron microscopy as well as for the phylogenetic analysis of endocytic bacteria. Bacterial aggregates are located in caverns of the tentacles’ epidermis. The aggregates are enwrapped in thin envelopes, which contain coccoid and/or rod-shaped tightly packed bacteria of different division states. Most of the bacterial cells are connected by fine filamentous structures. The phylogenetic determination is based on the sequence data of the 16S rDNA derived from tentacle material. Sequence analysis revealed three different subgroups of intratentacular proteobacteria. The dominant band, detected in all of the samples tested, showed a close relationship (98%) to a gram-negative Endozoicimonas elysicola. Two bands, only detected in tentacles of M. senile from Helgoland were assigned to Pseudomonas saccherophilia (99%), a knallgas bacterium, and to Ralstonia pickettii (100%). The bacteria represent a specific bacterial community. Their DGGE profiles do not correspond to the profiles of the planktonic bacteria generated from seawater close to the habitats of the anemones. The allocation of DNA sequences to the different morphotypes, their isolation, culturing and the elucidation of the physiological functions of intratentacular bacteria are in progress.

Schuett, Christian; Doepke, Hilke; Grathoff, Annette; Gedde, Michael

2007-09-01

63

Animating the Escape Response of the Sea Anemone, Stomphia Coccinea from the Starfish, Dermasterias imbricata Modeled Using Implicit Surfaces  

Microsoft Academic Search

Many of the most interesting simulations in the biological world have to do with interactions between species. The predator-prey interactions among aquatic organisms are an interesting part of the natural world which has not been seen much in computer animation. This paper explores the interaction between various sea anemones and the starfish Dermasterias imbricata. Although a simulation between a specific

Mai Ali Nur; Xikun Liang; Brian Wyvill; George B. Bourne

2001-01-01

64

Effects of Lipid Composition on Membrane Permeabilization by Sticholysin I and II, Two Cytolysins of the Sea Anemone Stichodactyla helianthus  

Microsoft Academic Search

Sticholysin I and II (St I and St II), two basic cytolysins purified from the Caribbean sea anemone Stichodactyla helianthus, efficiently permeabilize lipid vesicles by forming pores in their membranes. A general characteristic of these toxins is their preference for membranes containing sphingomyelin (SM). As a consequence, vesicles formed by equimolar mixtures of SM with phosphatidylcholine (PC) are very good

Carlos Alvarez Valcarcel; Mauro Dalla Serra; Cristina Potrich; Ivonne Bernhart; Mayra Tejuca; Diana Martinez; Fabiola Pazos; Maria E. Lanio; Gianfranco Menestrina

2001-01-01

65

Trace element profiles of the sea anemone Anemonia viridis living nearby a natural CO2 vent  

PubMed Central

Ocean acidification (OA) is not an isolated threat, but acts in concert with other impacts on ecosystems and species. Coastal marine invertebrates will have to face the synergistic interactions of OA with other global and local stressors. One local factor, common in coastal environments, is trace element contamination. CO2 vent sites are extensively studied in the context of OA and are often considered analogous to the oceans in the next few decades. The CO2 vent found at Levante Bay (Vulcano, NE Sicily, Italy) also releases high concentrations of trace elements to its surrounding seawater, and is therefore a unique site to examine the effects of long-term exposure of nearby organisms to high pCO2 and trace element enrichment in situ. The sea anemone Anemonia viridis is prevalent next to the Vulcano vent and does not show signs of trace element poisoning/stress. The aim of our study was to compare A. viridis trace element profiles and compartmentalization between high pCO2 and control environments. Rather than examining whole anemone tissue, we analyzed two different body compartments—the pedal disc and the tentacles, and also examined the distribution of trace elements in the tentacles between the animal and the symbiotic algae. We found dramatic changes in trace element tissue concentrations between the high pCO2/high trace element and control sites, with strong accumulation of iron, lead, copper and cobalt, but decreased concentrations of cadmium, zinc and arsenic proximate to the vent. The pedal disc contained substantially more trace elements than the anemone’s tentacles, suggesting the pedal disc may serve as a detoxification/storage site for excess trace elements. Within the tentacles, the various trace elements displayed different partitioning patterns between animal tissue and algal symbionts. At both sites iron was found primarily in the algae, whereas cadmium, zinc and arsenic were primarily found in the animal tissue. Our data suggests that A. viridis regulates its internal trace element concentrations by compartmentalization and excretion and that these features contribute to its resilience and potential success at the trace element-rich high pCO2 vent. PMID:25250210

Borell, Esther M.; Fine, Maoz; Shaked, Yeala

2014-01-01

66

Phospholipase A2 in cnidaria.  

PubMed

Phospholipase A2 (PLA2) is an enzyme present in snake and other venoms and body fluids. We measured PLA2 catalytic activity in tissue homogenates of 22 species representing the classes Anthozoa, Hydrozoa, Scyphozoa and Cubozoa of the phylum Cnidaria. High PLA2 levels were found in the hydrozoan fire coral Millepora sp. (median 735 U/g protein) and the stony coral Pocillopora damicornis (693 U/g) that cause skin irritation upon contact. High levels of PLA2 activity were also found in the acontia of the sea anemone Adamsia carciniopados (293 U/g). Acontia are long threads containing nematocysts and are used in defense and aggression by the animal. Tentacles of scyphozoan and cubozoan species had high PLA2 activity levels: those of the multitentacled box jellyfish Chironex fleckeri contained 184 U/g PLA2 activity. The functions of cnidarian PLA2 may include roles in the capture and digestion of prey and defense of the animal. The current observations support the idea that cnidarian PLA2 may participate in the sting site irritation and systemic envenomation syndrome resulting from contact with cnidarians. PMID:15581805

Nevalainen, Timo J; Peuravuori, Heikki J; Quinn, Ronald J; Llewellyn, Lyndon E; Benzie, John A H; Fenner, Peter J; Winkel, Ken D

2004-12-01

67

Polyoxygenated 24,28-epoxyergosterols inhibiting the proliferation of glioma cells from sea anemone Anthopleura midori.  

PubMed

Eleven sterols (1-11) and one carotenoid (12) were isolated and identified from sea anemone Anthopleura midori. Compounds 1-6 are rare polyoxygenated ergosterols with a 24,28-epoxy moiety. The structures of these epoxyergosterols were determined by NMR and HRESIMS analyses as well as their chemical-physical properties. Epoxyergosterols 1 and 2 were found to be new natural products and 3-6 are new compounds. Bioactive assay showed that compounds 1, 2, 3, 5, 7, 8, 11, and 12 inhibited the proliferation of rat glioma C6 and human glioma U251 cells with IC50 in a range of 2.41-80.45 ?M. Further investigation suggested that 1 and 3 induced apoptosis in glioma cells and 1 blocked U251 cells at the G0/G1 phase. PMID:24973636

Yu, Siran; Ye, Xuewei; Chen, Lu; Lian, Xiao-Yuan; Zhang, Zhizhen

2014-10-01

68

Computational Insights of the Interaction among Sea Anemones Neurotoxins and Kv1.3 Channel  

PubMed Central

Sea anemone neurotoxins are peptides that interact with Na+ and K+ channels, resulting in specific alterations on their functions. Some of these neurotoxins (1ROO, 1BGK, 2K9E, 1BEI) are important for the treatment of about 80 autoimmune disorders because of their specificity for Kv1.3 channel. The aim of this study was to identify the common residues among these neurotoxins by computational methods, and establish whether there is a pattern useful for the future generation of a treatment for autoimmune diseases. Our results showed eight new key common residues between the studied neurotoxins interacting with a histidine ring and the selectivity filter of the receptor, thus showing a possible pattern of interaction. This knowledge may serve as an input for the design of more promising drugs for autoimmune treatments. PMID:24812496

Sabogal-Arango, Angélica; Barreto, George E; Ramírez-Sánchez, David; González-Mendoza, Juan; Barreto, Viviana; Morales, Ludis; González, Janneth

2014-01-01

69

Characterization of Circadian Behavior in the Starlet Sea Anemone, Nematostella vectensis  

PubMed Central

Background Although much is known about how circadian systems control daily cycles in the physiology and behavior of Drosophila and several vertebrate models, marine invertebrates have often been overlooked in circadian rhythms research. This study focuses on the starlet sea anemone, Nematostella vectensis, a species that has received increasing attention within the scientific community for its potential as a model research organism. The recently sequenced genome of N. vectensis makes it an especially attractive model for exploring the molecular evolution of circadian behavior. Critical behavioral data needed to correlate gene expression patterns to specific behaviors are currently lacking in N. vectensis. Methodology/Principal Findings To detect the presence of behavioral oscillations in N. vectensis, locomotor activity was evaluated using an automated system in an environmentally controlled chamber. Animals exposed to a 24 hr photoperiod (12 hr light: 12 hr dark) exhibited locomotor behavior that was both rhythmic and predominantly nocturnal. The activity peak occurred in the early half of the night with a 2-fold increase in locomotion. Upon transfer to constant lighting conditions (constant light or constant dark), an approximately 24 hr rhythm persisted in most animals, suggesting that the rhythm is controlled by an endogenous circadian mechanism. Fourier analysis revealed the presence of multiple peaks in some animals suggesting additional rhythmic components could be present. In particular, an approximately 12 hr oscillation was often observed. The nocturnal increase in generalized locomotion corresponded to a 24 hr oscillation in animal elongation. Conclusions/Significance These data confirm the presence of a light-entrainable circadian clock in Nematostella vectensis. Additional components observed in some individuals indicate that an endogenous clock of approximately 12 hr frequency may also be present. By describing rhythmic locomotor behavior in N. vectensis, we have made important progress in developing the sea anemone as a model organism for circadian rhythm research. PMID:23056482

Hendricks, William D.; Byrum, Christine A.; Meyer-Bernstein, Elizabeth L.

2012-01-01

70

A muscle-specific transgenic reporter line of the sea anemone, Nematostella vectensis.  

PubMed

The sea anemone, Nematostella vectensis, has become an attractive new model organism for comparative genomics and evolutionary developmental biology. Over the last few years, many genes have been isolated and their expression patterns studied to gain insight into their function. More recently, functional tools have been developed to manipulate gene function; however, most of these approaches rely on microinjection and are limited to early stages of development. Transgenic lines would significantly enhance the tractability of the system. In particular, the study of gene- or tissue-specific promoters would be most useful. Here we report the stable establishment of a transgenic line using the I-SceI meganuclease system to facilitate integration into the genome. We isolated a 1.6-kb fragment of the regulatory upstream region of the Myosin Heavy Chain1 (MyHC1) gene and found that the transgene is specifically expressed in the retractor and tentacle muscles of Nematostella polyps, faithfully reproducing the expression of the endogenous MyHC1 gene. This demonstrates that the 1.6-kb fragment contains all of the regulatory elements necessary to drive correct expression and suggests that retractor and tentacle muscles in Nematostella are distinct from other myoepithelial cells. The transgene is transmitted through the germline at high frequency, and G(1) transgenic polyps have only one integration site. The relatively high frequency of transgenesis, in combination with gene- or tissue-specific promoters, will foster experimental possibilities for studying in vivo gene functions in gene regulatory networks and developmental processes in the nonbilaterian sea anemone, Nematostella vectensis. PMID:20018670

Renfer, Eduard; Amon-Hassenzahl, Annette; Steinmetz, Patrick R H; Technau, Ulrich

2010-01-01

71

Modulation of neuronal sodium channels by the sea anemone peptide BDS-I  

PubMed Central

Blood-depressing substance I (BDS-I), a 43 amino-acid peptide from sea anemone venom, is used as a specific inhibitor of Kv3-family potassium channels. We found that BDS-I acts with even higher potency to modulate specific types of voltage-dependent sodium channels. In rat dorsal root ganglion (DRG) neurons, 3 ?M BDS-I strongly enhanced tetrodotoxin (TTX)-sensitive sodium current but weakly inhibited TTX-resistant sodium current. In rat superior cervical ganglion (SCG) neurons, which express only TTX-sensitive sodium current, BDS-I enhanced current elicited by small depolarizations and slowed decay of currents at all voltages (EC50 ? 300 nM). BDS-I acted with exceptionally high potency and efficacy on cloned human Nav1.7 channels, slowing inactivation by 6-fold, with an EC50 of approximately 3 nM. BDS-I also slowed inactivation of sodium currents in N1E-115 neuroblastoma cells (mainly from Nav1.3 channels), with an EC50 ? 600 nM. In hippocampal CA3 pyramidal neurons (mouse) and cerebellar Purkinje neurons (mouse and rat), BDS-I had only small effects on current decay (slowing inactivation by 20–50%), suggesting relatively weak sensitivity of Nav1.1 and Nav1.6 channels. The biggest effect of BDS-I in central neurons was to enhance resurgent current in Purkinje neurons, an effect reflected in enhancement of sodium current during the repolarization phase of Purkinje neuron action potentials. Overall, these results show that BDS-I acts to modulate sodium channel gating in a manner similar to previously known neurotoxin receptor site 3 anemone toxins but with different isoform sensitivity. Most notably, BDS-I acts with very high potency on human Nav1.7 channels. PMID:22442564

Liu, Pin; Jo, Sooyeon

2012-01-01

72

The sea anemone toxin AdE-1 modifies both sodium and potassium currents of rat cardiomyocytes.  

PubMed

AdE-1, a cardiotonic peptide recently isolated from the sea anemone Aiptasia diaphana, contains 44 amino acids and has a molecular mass of 4907 Da. It was previously found to resemble other sea anemone type 1 and 2 Na+ channel toxins, enhancing contractions of rat cardiomyocytes and slowing their twitch relaxation; however, it did not induce spontaneous twitches. AdE-1 increased the duration of the cardiomyocyte action potential and decreased its amplitude and its time-to-peak in a concentration-dependent manner, without affecting its threshold and cell resting potential. Nor did it generate the early and delayed after-depolarizations characteristic of sea anemone Na+ channel toxins. To further understand its mechanism of action we investigated the effect of AdE-1 on the major ion currents of rat cardiomyocytes. In the present study we show that AdE-1 markedly slowed inactivation of the Na+ current, enhancing and prolonging the current influx with no effect on current activation, possibly through direct interaction with the site 3 receptor of the Na+ channel. No significant effect of AdE-1 on the Ca2+ current was observed, but, unexpectedly, AdE-1 significantly increased the amplitude of the transient component of the K+ current, shifting the current threshold to more negative membrane potentials. This effect on the K+ current has not been found in any other sea anemone toxin and may explain the exclusive reduction in action potential amplitude and the absence of the action potential disorders found with other toxins, such as early and delayed after-depolarizations. PMID:24749540

Nesher, Nir; Zlotkin, Eliahu; Hochner, Binyamin

2014-07-01

73

Lipid composition of beef brain, beef liver, and the sea anemone: Two approaches to quantitative fractionation of complex lipid mixtures  

Microsoft Academic Search

Two new schemes for fractionation of complex lipid mixtures are presented. Their use for the study of lipids of beef brain,\\u000a beef liver, and the sea anemone are described. Apparatus and techniques for working in an inert atmosphere, evaporation of\\u000a solutions in the cold under nitrogen, use of infrared spectroscopy for examination of lipids and their hydrolysis products,\\u000a preparation and

George Rouser; Gene Kritchevsky; Dorothy Heller; Ellen Lieber

1963-01-01

74

Reproductive biology of the sea anemone shrimp Periclimenes rathbunae (Caridea, Palaemonidae, Pontoniinae), from the Caribbean coast of Costa Rica  

PubMed Central

Abstract Caridean shrimps are a highly diverse group and many species form symbiotic relationships with different marine invertebrates. Periclimenes rathbunae is a brightly colored shrimp that lives predominantly in association with sea anemones. Information about the reproductive ecology of the species is scarce. Therefore, we collected 70 ovigerous females inhabiting the sun sea anemone Stichodactyla helianthus in coral reefs from the southern Caribbean coast of Costa Rica. Females produced on average 289 ± 120 embryos. The volume of recently-produced embryos was on average 0.038 mm3, and embryo volume increased by 192% during the incubation period. The average embryo mortality during embryogenesis was 24%. The reproductive output was 0.24 ± 0.094, considerably higher than in many other pontoniine shrimps. Females carrying embryos close to hatching showed fully developed ovaries, suggesting consecutive spawning. We assume that the sheltered habitat, living on sea anemones, allows Periclimenes rathbunae to allocate more energy in embryo production than most other free-living caridean shrimps. This is the first record of Periclimenes rathbunae for Costa Rica. PMID:25561838

Azofeifa-Solano, Juan Carlos; Elizondo-Coto, Marcelo; Wehrtmann, Ingo S.

2014-01-01

75

Oxidative stress and apoptotic events during thermal stress in the symbiotic sea anemone, Anemonia viridis.  

PubMed

Symbiosis between cnidarian and photosynthetic protists is widely distributed over temperate and tropical seas. These symbioses can periodically breakdown, a phenomenon known as cnidarian bleaching. This event can be irreversible for some associations subjected to acute and/or prolonged environmental disturbances, and leads to the death of the animal host. During bleaching, oxidative stress has been described previously as acting at molecular level and apoptosis is suggested to be one of the mechanisms involved. We focused our study on the role of apoptosis in bleaching via oxidative stress in the association between the sea anemone Anemonia viridis and the dinoflagellates Symbiodinium species. Characterization of caspase-like enzymes were conducted at the biochemical and molecular level to confirm the presence of a caspase-dependent apoptotic phenomenon in the cnidarian host. We provide evidence of oxidative stress followed by induction of caspase-like activity in animal host cells after an elevated temperature stress, suggesting the concomitant action of these components in bleaching. PMID:16907933

Richier, Sophie; Sabourault, Cécile; Courtiade, Juliette; Zucchini, Nathalie; Allemand, Denis; Furla, Paola

2006-09-01

76

Algal genotype and photoacclimatory responses of the symbiotic alga Symbiodinium in natural populations of the sea anemone Anemonia viridis  

PubMed Central

As an approach to investigate the impact of solar radiation on an alga–invertebrate symbiosis, the genetic variation and photosynthetic responses of the dinoflagellate algal symbiosis in an intertidal and a subtidal population of the sea anemone Anemonia viridis were explored. Allozyme analysis of the anemones indicated that the two populations were genetically very similar, with a Nei's index value of genetic identity (I) of 0.998. The algae in all animals examined were identified as Symbiodinium of clade a by PCR-RFLP analysis of the small subunit ribosomal RNA gene. The symbiosis in the two populations did not differ significantly in algal population density, chlorophyll a content per algal cell or any photosynthetic parameter obtained from studies of the relationship between photosynthesis and irradiance. We conclude that there is not necessarily genetic variation or photosynthetic plasticity of the symbiotic algae in Anemonia viridis inhabiting environments characterized by the different solar irradiances of the subtidal and intertidal habitats.

Bythell, J. C.; Douglas, A. E.; Sharp, V. A.; Searle, J. B.; Brown, B. E.

1997-01-01

77

Antifouling and Fungicidal Resorcylic Acid Lactones from the Sea Anemone-Derived Fungus Cochliobolus lunatus.  

PubMed

Three new 14-membered resorcylic acid lactones, cochliomycins D-F, 1-3, and eight known analogues, 4-11, were isolated from the sea anemone-derived fungus Cochliobolus lunatus. Compounds 1-4 are diastereomers differing from each other by the absolute configurations of the 4',5'-diol chiral centers. The absolute configurations of 1-4 were established by the CD exciton chirality method and TDDFT ECD calculations. In antifouling assays, 1, 3-6, and 6a exhibited potent antifouling activities against the larval settlement of the barnacle Balanus amphitrite at nontoxic concentrations, with EC50 values ranging from 1.82 to 22.5 ?g/mL. Noticeably, fungicide whole-plant assays indicated that 6 showed excellent activity on the Plasmopara viticola preventative test at 6 ppm and concentration-dependent activity on the Phytophthora infestans preventative application at 200, 60, and 20 ppm. Preliminary structure-activity relationships are also discussed. PMID:24635109

Liu, Qing-Ai; Shao, Chang-Lun; Gu, Yu-Cheng; Blum, Mathias; Gan, Li-She; Wang, Kai-Ling; Chen, Min; Wang, Chang-Yun

2014-03-25

78

Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea  

PubMed Central

A new Cytolysin, termed as Gigantoxin-4, was isolated from the sea anemone Stichodactyla gigantea and found to be highly homologous with Cytolysin-3 (HMg III) from Heteractis magnifica, RTX-A from Radianthus macrodactylus, and Sticholysin-1 (St I) and Sticholysin-2 (St II) from Stichodactyla helianthus (homology 82%, 86%, 82% and 86% respectively). Its 20 N-terminal residues were identified and the full-length cDNA sequence was obtained by reverse transcription-polymerase chain reaction (RT-PCR). Multiple sequence alignments with other Cytolysins of the actinoporin family clearly indicated that Gigantoxin-4 belongs to this protein family. SDS-PAGE electrophoresis showed that this new actinoporin had a molecular mass of about 19 kDa, and possessed a high hemolytic activity to human erythrocytes (HA50= 40 ng/ml), which was inhibited by pre-incubation with sphingomyelin (SM) or SM-cholesterol mixtures. Our in vivo experiments showed that Gigantoxin-4 had wide toxicity to the rat cardiovascular system and the respiratory system. A concentration of 30?g/kg Gigantoxin-4, i.v. produced a positive inotropic effect on the rat heart although final cardiovascular failure was inevitable, and 60?g/kg Gigantoxin-4 caused respiratory arrest rapidly resulting in rat death. HE staining indicated pathological changes in various organs and tissues after i.v. administration of Gigantoxin-4. PMID:21697999

Hu, Bo; Guo, Wei; Wang, Liang-hua; Wang, Jian-guang; Liu, Xiao-yu; Jiao, Bing-hua

2011-01-01

79

Hct-A Is a New Actinoporin Family from the Heteractis Crispa Sea Anemone  

PubMed Central

Several new actinoporin isoforms with molecular weights of 18995.5 to 19398.7 Da exhibiting a high hemolytic activity were isolated from the tropical sea anemone Heteractis crispa using a combination of liquid chromatography techniques. The actinoporins were demonstrated to occur as mono-, di-, and trimers in aqueous solutions. The sequences of the genes encoding actinoporins were identified, and the amino acid sequences of the new polypeptides belonging to the Hct-A actinoporin family were obtained. The new acinoporins differ in their isoelectric points, the number and localization of charged amino acid residues at the functionally important N-terminal fragment of the molecule, as well as in the charge of a tetrapeptide (amino acid residues 74–77) involved in an electrostatic interaction with the cytoplasmic membrane. A recombinant actinoporin, rHct-A2, with a molecular weight of 19141 Da, pI of 9.64, and hemolytic activity of 4.0 × 104 HU/mg, was obtained. The conductivity of the ion channels formed by rHct-A2 in the BLM was demonstrated to be similar to that of the native actinoporin from H. crispa. The obtained data expand knowledge on the structural and functional relationships of actinoporins and contribute to our understanding of the functioning mechanism of these molecules, which is the basis for the development of compounds with a high biomedical potential. Currently, they are considered as models for obtaining antitumor, antibacterial, and cardiac-stimulating agents.

Leichenko, E. V.; Monastirnaya, M. M.; Zelepuga, E. A.; Tkacheva, E. S.; Isaeva, M. P.; Likhatskaya, G. N.; Anastyuk, S. D.; Kozlovskaya, E. P.

2014-01-01

80

Fast Neurotransmission Related Genes Are Expressed in Non Nervous Endoderm in the Sea Anemone Nematostella vectensis  

PubMed Central

Cnidarian nervous systems utilize chemical transmission to transfer signals through synapses and neurons. To date, ample evidence has been accumulated for the participation of neuropeptides, primarily RFamides, in neurotransmission. Yet, it is still not clear if this is the case for the classical fast neurotransmitters such as GABA, Glutamate, Acetylcholine and Monoamines. A large repertoire of cnidarian Fast Neurotransmitter related Genes (FNGs) has been recently identified in the genome of the sea anemone, Nematostella vectensis. In order to test whether FNGs are localized in cnidarian neurons, we characterized the expression patterns of eight Nematostella genes that are closely or distantly related to human central and peripheral nervous systems genes, in adult Nematostella and compared them to the RFamide localization. Our results show common expression patterns for all tested genes, in a single endodermal cell layer. These expressions did not correspond with the RFamide expressing nerve cell network. Following these results we suggest that the tested Nematostella genes may not be directly involved in vertebrate-like fast neurotransmission. PMID:24705400

Oren, Matan; Brikner, Itzchak; Appelbaum, Lior; Levy, Oren

2014-01-01

81

Interactions between the tropical sea anemone Aiptasia pallida and Serratia marcescens, an opportunistic pathogen of corals.  

PubMed

Coral reefs are under increasing stress caused by global and local environmental changes, which are thought to increase the susceptibility of corals to opportunistic pathogens. In the absence of an easily culturable model animal, the understanding of the mechanisms of disease progression in corals remains fairly limited. In the present study, we tested the susceptibility of the tropical sea anemone Aiptasia pallida to an opportunistic coral pathogen (Serratia marcescens). A.?pallida was susceptible to S. marcescens?PDL100 and responded to this opportunistic coral pathogen with darkening of the tissues and retraction of tentacles, followed by complete disintegration of polyp tissues. Histological observations revealed loss of zooxanthellae and structural changes in eosinophilic granular cells in response to pathogen infection. A screen of S.?marcescens mutants identified a motility and tetrathionate reductase mutants as defective in virulence in the A.?pallida infection model. In co-infections with the wild-type strain, the tetrathionate reductase mutant was less fit within the surface mucopolysaccharide layer of the host coral Acropora palmata. PMID:24983533

Krediet, Cory J; Meyer, Julie L; Gimbrone, Nicholas; Yanong, Roy; Berzins, Ilze; Alagely, Ali; Castro, Herman; Ritchie, Kim B; Paul, Valerie J; Teplitski, Max

2014-06-01

82

Outcomes of infections of sea anemone Aiptasia pallida with Vibrio spp. pathogenic to corals.  

PubMed

Incidents of coral disease are on the rise. However, in the absence of a surrogate animal host, understanding of the interactions between coral pathogens and their hosts remains relatively limited, compared to other pathosystems of similar global importance. A tropical sea anemone, Aiptasia pallida, has been investigated as a surrogate model to study certain aspects of coral biology. Therefore, to test whether the utility of this surrogate model can be extended to study coral diseases, in the present study, we tested its susceptibility to common coral pathogens (Vibrio coralliilyticus and Vibrio shiloi) as well as polymicrobial consortia recovered from the Caribbean Yellow Band Disease (CYBD) lesions. A. pallida was susceptible to each of the tested pathogens. A. pallida responded to the pathogens with darkening of the tissues (associated with an increased melanization) and retraction of tentacles, followed by complete disintegration of polyp tissues. Loss of zooxanthellae was not observed; however, the disease progression pattern is consistent with the behavior of necrotizing pathogens. Virulence of some coral pathogens in Aiptasia was paralleled with their glycosidase activities. PMID:24619233

Zaragoza, William J; Krediet, Cory J; Meyer, Julie L; Canas, Gabriela; Ritchie, Kim B; Teplitski, Max

2014-08-01

83

Fatty Acid and Phospholipid Syntheses Are Prerequisites for the Cell Cycle of Symbiodinium and Their Endosymbiosis within Sea Anemones  

PubMed Central

Lipids are a source of metabolic energy, as well as essential components of cellular membranes. Although they have been shown to be key players in the regulation of cell proliferation in various eukaryotes, including microalgae, their role in the cell cycle of cnidarian-dinoflagellate (genus Symbiodinium) endosymbioses remains to be elucidated. The present study examined the effects of a lipid synthesis inhibitor, cerulenin, on the cell cycle of both cultured Symbiodinium (clade B) and those engaged in an endosymbiotic association with the sea anemone Aiptasia pulchella. In the former, cerulenin exposure was found to inhibit free fatty acid (FFA) synthesis, as it does in other organisms. Additionally, while it also significantly inhibited the synthesis of phosphatidylethanolamine (PE), it did not affect the production of sterol ester (SE) or phosphatidylcholine (PC). Interestingly, cerulenin also significantly retarded cell division by arresting the cell cycles at the G0/G1 phase. Cerulenin-treated Symbiodinium were found to be taken up by anemone hosts at a significantly depressed quantity in comparison with control Symbiodinium. Furthermore, the uptake of cerulenin-treated Symbiodinium in host tentacles occurred much more slowly than in untreated controls. These results indicate that FFA and PE may play critical roles in the recognition, proliferation, and ultimately the success of endosymbiosis with anemones. PMID:24009685

Wang, Li-Hsueh; Lee, Hsieh-He; Fang, Lee-Shing; Mayfield, Anderson B.; Chen, Chii-Shiarng

2013-01-01

84

Ancient origins of axial patterning genes: Hox genes and ParaHox genes in the Cnidaria.  

PubMed

Among the bilaterally symmetrical, triploblastic animals (the Bilateria), a conserved set of developmental regulatory genes are known to function in patterning the anterior-posterior (AP) axis. This set includes the well-studied Hox cluster genes, and the recently described genes of the ParaHox cluster, which is believed to be the evolutionary sister of the Hox cluster (Brooke et al. 1998). The conserved role of these axial patterning genes in animals as diverse as frogs and flies is believed to reflect an underlying homology (i.e., all bilaterians derive from a common ancestor which possessed an AP axis and the developmental mechanisms responsible for patterning the axis). However, the origin and early evolution of Hox genes and ParaHox genes remain obscure. Repeated attempts have been made to reconstruct the early evolution of Hox genes by analyzing data from the triphoblastic animals, the Bilateria (Schubert et al. 1993; Zhang and Nei 1996). A more precise dating of Hox origins has been elusive due to a lack of sufficient information from outgroup taxa such as the phylum Cnidaria (corals, hydras, jellyfishes, and sea anemones). In combination with outgroup taxa, another potential source of information about Hox origins is outgroup genes (e.g., the genes of the ParaHox cluster). In this article, we present cDNA sequences of two Hox-like genes (anthox2 and anthox6) from the sea anemone, Nematostella vectensis. Phylogenetic analysis indicates that anthox2 (= Cnox2) is homologous to the GSX class of ParaHox genes, and anthox6 is homologous to the anterior class of Hox genes. Therefore, the origin of Hox genes and ParaHox genes occurred prior to the evolutionary split between the Cnidaria and the Bilateria and predated the evolution of the anterior-posterior axis of bilaterian animals. Our analysis also suggests that the central Hox class was invented in the bilaterian lineage, subsequent to their split from the Cnidaria. PMID:11324016

Finnerty, J R; Martindale, M Q

1999-01-01

85

Changes of cellular superficial configuration of symbiotic algae during cultivation from two anemones found in the South China Sea  

NASA Astrophysics Data System (ADS)

Symbiotic algae from two anemones, Radianthus macrodactylus and Stichodactyla mertensii, found in the South China Sea, were cultivated in ASP-8A medium in this study. Changes of superficial configuration of symbiotic algae during the cultivation were studied by means of a microscope and a scanning electron microscope (SEM). A number of small cavities appeared on the surfaces of symbiotic algae after they were cultivated for 10 h. The cavities enlarged and the cell contents were lost with extended cultivation. Our data suggested that the presence of cavities on symbiotic algae surfaces may be one of the main reasons for failure to culture symbiotic algae in an artificial medium.

Zhu, Baohua; Pan, Kehou; Wang, Guangce

2008-02-01

86

Effects of sea anemone (Heteractis magnifica and Actinia equina) cytolysins on synaptosomal uptake of GABA and choline.  

PubMed

Magnificalysin I and II (HMg I and II) and equinatoxin II (EqTx II) are cytolytic toxins extracted from sea anemones Heteractis magnifica and Actinia equina, respectively. They induced haemolysis in rat red blood cells and inhibited gamma amino butyric acid (GABA) and choline uptake into rat brain synaptosomes. These effects were concentration dependent. The inhibition of GABA and choline uptake could be overcome by the addition of exogenous sphingomyelin, suggesting that there might be interaction between these cytolysins and the phospholipid. Although the precise mechanisms involved in haemolysis and inhibition of GABA and choline uptake are unknown, they appeared to be different. PMID:8599187

Khoo, H E; Lim, J P; Tan, C H

1995-10-01

87

Functional polarity of the tentacle of the sea anemone Anemonia viridis: role in inorganic carbon acquisition.  

PubMed

The oral epithelial layers of anthozoans have a polarized morphology: photosynthetic endosymbionts live within endodermal cells facing the coelenteric cavity and are separated from the external seawater by the ectodermal layer and the mesoglea. To study if this morphology plays a role in the supply of inorganic carbon for symbiont photosynthesis, we measured the change in pH and the rate of OH- (H+) fluxes induced by each cell layer on a tentacle of the sea anemone Anemonia viridis. Light-induced pH increase of the medium bathing the endodermal layers led to the generation of a transepithelial pH gradient of approximately 0.8 pH units across the tentacle, whereas darkness induced acidification of this medium. The light-induced pH change was associated with an increase of total alkalinity. Only the endodermal layer was able to induce a net OH- secretion (H+ absorption). The light-induced OH- secretion by the endodermal cell layer was dependent on the presence of HCO3- in the compartment facing the ectoderm and was sensitive to several inhibitors of ion transport. [14C] HCO3- incorporation into photosynthates confirmed the ectodermal supply, the extent of which varied from 25 to > 90%, according to HCO3- availability. Our results suggest that the light-induced OH- secretion by the endodermal cell layer followed the polarized transport of HCO3- and its subsequent decarboxylation within the endodermal cell layer. This polarity may play a significant role both in inorganic carbon absorption and in the control of light-enhanced calcification in scleractinian corals. PMID:9486285

Furla, P; Bénazet-Tambutté, S; Jaubert, J; Allemand, D

1998-02-01

88

Comparative anatomy and histology of developmental and parasitic stages in the life cycle of the lined sea anemone Edwardsiella lineata.  

PubMed

The evolution of parasitism is often accompanied by profound changes to the developmental program. However, relatively few studies have directly examined the developmental evolution of parasitic species from free-living ancestors. The lined sea anemone Edwardsiella lineata is a relatively recently evolved parasite for which closely related free-living outgroups are known, including the starlet sea anemone Nematostella vectensis. The larva of E. lineata parasitizes the ctenophore Mnemiopsis leidyi, and, once embedded in its host, the anemone assumes a novel vermiform body plan. That we might begin to understand how the developmental program of this species has been transformed during the evolution of parasitism, we characterized the gross anatomy, histology, and cnidom of the parasitic stage, post-parasitic larval stage, and adult stage of the E. lineata life cycle. The distinct parasitic stage of the life cycle differs from the post-parasitic larva with respect to overall shape, external ciliation, cnida frequency, and tissue architecture. The parasitic stage and planula both contain holotrichs, a type of cnida not previously reported in Edwardsiidae. The internal morphology of the post-parasitic planula is extremely similar to the adult morphology, with a complete set of mesenterial tissue and musculature despite this stage having little external differentiation. Finally, we observed 2 previously undocumented aspects of asexual reproduction in E. lineata: (1) the parasitic stage undergoes transverse fission via physal pinching, the first report of asexual reproduction in a pre-adult stage in the Edwardsiidae; and (2) the juvenile polyp undergoes transverse fission via polarity reversal, the first time this form of fission has been reported in E. lineata. PMID:18656992

Reitzel, Adam M; Daly, Marymegan; Sullivan, James C; Finnerty, John R

2009-02-01

89

Sea Anemone Peptide with Uncommon ?-Hairpin Structure Inhibits Acid-sensing Ion Channel 3 (ASIC3) and Reveals Analgesic Activity*  

PubMed Central

Three novel peptides were isolated from the venom of the sea anemone Urticina grebelnyi. All of them are 29 amino acid peptides cross-linked by two disulfide bridges, with a primary structure similar to other sea anemone peptides belonging to structural group 9a. The structure of the gene encoding the shared precursor protein of the identified peptides was determined. One peptide, ?-AnmTX Ugr 9a-1 (short name Ugr 9-1), produced a reversible inhibition effect on both the transient and the sustained current of human ASIC3 channels expressed in Xenopus laevis oocytes. It completely blocked the transient component (IC50 10 ± 0.6 ?m) and partially (48 ± 2%) inhibited the amplitude of the sustained component (IC50 1.44 ± 0.19 ?m). Using in vivo tests in mice, Ugr 9-1 significantly reversed inflammatory and acid-induced pain. The other two novel peptides, AnmTX Ugr 9a-2 (Ugr 9-2) and AnmTX Ugr 9a-3 (Ugr 9-3), did not inhibit the ASIC3 current. NMR spectroscopy revealed that Ugr 9-1 has an uncommon spatial structure, stabilized by two S-S bridges, with three classical ?-turns and twisted ?-hairpin without interstrand disulfide bonds. This is a novel peptide spatial structure that we propose to name boundless ?-hairpin. PMID:23801332

Osmakov, Dmitry I.; Kozlov, Sergey A.; Andreev, Yaroslav A.; Koshelev, Sergey G.; Sanamyan, Nadezhda P.; Sanamyan, Karen E.; Dyachenko, Igor A.; Bondarenko, Dmitry A.; Murashev, Arkadii N.; Mineev, Konstantin S.; Arseniev, Alexander S.; Grishin, Eugene V.

2013-01-01

90

Incorporating fine-scale seascape composition in an assessment of habitat quality for the giant sea anemone Stichodactyla gigantea in a coral reef shore zone  

Microsoft Academic Search

Habitat loss due to land reclamation often occurs in sandy coral reef shore zones. The giant sea anemone Stichodactyla gigantea, which harbors the false clown anemonefish Amphiprion ocellaris, both of which are potentially flagship species, inhabit these places. To assess habitat quality for S. gigantea, we examined correlative associations between the number and the body size of S. gigantea and

Akihisa Hattori; Miyako Kobayashi

2009-01-01

91

The influence of salinity and copper exposure on copper accumulation and physiological impairment in the sea anemone, Exaiptasia pallida.  

PubMed

Copper is a common pollutant in many aquatic environments, particularly those surrounding densely populated areas with substantial anthropogenic inputs. These same areas may also experience changes in salinity due to freshwater discharge and tidal influence. Biota that inhabit near-shore coastal environments may be susceptible to both stressors. Although copper is a noted concern in marine environments, effects of copper and varying salinity on symbiotic cnidarians are only scarcely studied. The sea anemone, Exaiptasia pallida, was used to investigate effects of copper on physiological impairment (i.e. activities of anti-oxidant enzymes) at two different salinities (20 and 25ppt). E. pallida was exposed to a control and three elevated copper concentrations for up to 21d, and copper accumulation and activity of the enzymes: catalase, glutathione reductase, glutathione peroxidase, and carbonic anhydrase were measured in the anemones. Photosynthetic parameters in E. pallida's symbiotic dinoflagellate algae were also quantified. Over the course of the exposure, E. pallida accumulated copper in a concentration-dependent manner. Higher tissue copper concentrations were observed in anemones exposed to the lower salinity water (20ppt), and physiological impairment was observed as a consequence of both increased copper exposure and decreased salinity; however, changes in salinity caused a greater response than copper exposure, at the levels tested. In general, antioxidant enzyme activity increased as a consequence of decreased salinity and/or increased copper exposure. These results clearly demonstrated the influence of two local stressors, at environmentally realistic concentrations, on a sensitive cnidarian, and highlight the importance of characterizing combined exposure scenarios. PMID:25451077

Patel, Pratikkumar P; Bielmyer-Fraser, Gretchen K

2015-02-01

92

Expression and mutagenesis of the sea anemone toxin Av2 reveals key amino acid residues important for activity on voltage-gated sodium channels.  

PubMed

Type I sea anemone toxins are highly potent modulators of voltage-gated Na-channels (Na(v)s) and compete with the structurally dissimilar scorpion alpha-toxins on binding to receptor site-3. Although these features provide two structurally different probes for studying receptor site-3 and channel fast inactivation, the bioactive surface of sea anemone toxins has not been fully resolved. We established an efficient expression system for Av2 (known as ATX II), a highly insecticidal sea anemone toxin from Anemonia viridis (previously named A. sulcata), and mutagenized it throughout. Each toxin mutant was analyzed in toxicity and binding assays as well as by circular dichroism spectroscopy to discern the effects derived from structural perturbation from those related to bioactivity. Six residues were found to constitute the anti-insect bioactive surface of Av2 (Val-2, Leu-5, Asn-16, Leu-18, and Ile-41). Further analysis of nine Av2 mutants on the human heart channel Na(v)1.5 expressed in Xenopus oocytes indicated that the bioactive surfaces toward insects and mammals practically coincide but differ from the bioactive surface of a structurally similar sea anemone toxin, Anthopleurin B, from Anthopleura xanthogrammica. Hence, our results not only demonstrate clear differences in the bioactive surfaces of Av2 and scorpion alpha-toxins but also indicate that despite the general conservation in structure and importance of the Arg-14 loop and its flanking residues Gly-10 and Gly-20 for function, the surface of interaction between different sea anemone toxins and Na(v)s varies. PMID:16846229

Moran, Yehu; Cohen, Lior; Kahn, Roy; Karbat, Izhar; Gordon, Dalia; Gurevitz, Michael

2006-07-25

93

From Sea Anemone to Homo Sapiens: The Evolution of the p53 Family of Genes  

SciTech Connect

The human genome contains three transcription factors termed p53, p63 and p73 which are related orthologues. The function of the p53 protein is to respond to a wide variety of stresses which can disrupt the fidelity of DNA replication and cell division in somatic cells of the body. These stress signals, such as DNA damage, increase the mutation rate during DNA duplication and so an active p53 protein responds by eliminating clones of cells with mutations employing apoptosis, senescence or cell cycle arrest. In this way the p53 protein acts as a tumor suppressor preventing the mutations that can lead to cancers. The p63 and p73 proteins act in a similar fashion to protect the germ line cells in females (eggs). In addition the p63 protein plays a central role in the formation of epithelial cell layers and p73 plays a critical role in the formation of several structures in the central nervous system. Based upon their amino acid sequences and structural considerations the oldest organisms that contain an ancestor of the p53/p63/p73 gene are the sea anemone or hydra. The present day representatives of these animals contain a p63/p73 like ancestor gene and the protein functions in germ cells of this animal to enforce the fidelity of DNA replication after exposure to ultraviolet light. Thus the structure and functions of this gene family have been preserved for over one billion years of evolution. Other invertebrates such as the worm, the fly and the clam contain a very similar ancestor gene with a similar set of functions. The withdrawal of a food source from a worm results in the p63/p73 mediated apoptosis of the eggs so that new organisms will not be hatched into a poor environment. A similar response is thought to occur in humans. Thus this ancestor gene ensures the fidelity of the next generation of organisms. The first time a clearly distinct new p53 gene arises is in the cartilaginous fish and in the bony fish a separation of the p

Levine, Arnold (Institute for Advanced Study) [Institute for Advanced Study

2009-09-14

94

Trioecy, a unique breeding strategy in the sea anemone Aiptasia diaphana and its association with sex steroids.  

PubMed

Reproductive development of anthozoans reveals wide range of breeding strategies. Here, we report the occurrence of trioecy in the sea anemone Aiptasia diaphana (co-occurrence of males, females, and hermaphrodites), which so far was well documented only in plants. Age-homogeneous populations were obtained from pedal lacerates (asexual propagules) and cultured under control conditions. Careful documentation of growth, gamete morphology, and vertebrate-like steroid (i.e., progesterone, testosterone, and estradiol) levels were carried out over a 9-wk period between 4 and 12 wk postlaceration (wpl). First phenotypic signs of gametes development were observed in 6-wk-old anemones, pointing to the differentiation of males and hermaphrodites. While the males exhibited cellular progression of spermatogenesis, the hermaphrodites underwent a process of sex allocation, giving rise to male, female, and hermaphrodite phenotypes. Testosterone levels were relatively high prior to gamete appearance (4 wpl) and later on during gamete maturation (10 wpl). Conversely, estradiol levels steadily increased from 6 to 10 wpl, reaching their peak concomitant with oocyte maturation. Interestingly, increased oocyte atresia incidences were recorded during 9-12 wpl, coinciding with declining levels of steroid hormones. These results point to a strong similarity between the activity of sex steroids in vertebrates and that of vertebrate-like sex steroids on critical stages of A. diaphana's sexual differentiation and gametogenic cycle. The reproductive characteristics of A. diaphana make this anthozoan an important model species for the study of evolutionary drivers and processes underlying sexual development. PMID:24790160

Armoza-Zvuloni, Rachel; Kramarsky-Winter, Esti; Loya, Yossi; Schlesinger, Ami; Rosenfeld, Hanna

2014-06-01

95

Population impacts of collecting sea anemones and anemonefish for the marine aquarium trade in the Philippines  

Microsoft Academic Search

Tropical marine ornamentals comprise an increasingly important fishery worldwide. Although the potential for overexploitation\\u000a of marine ornamentals is great, few studies have addressed the population-level impacts of ornamental exploitation and few\\u000a ornamental fisheries are managed. Analysis of catch records obtained from collectors over a four-month period in the vicinity\\u000a of Cebu, Philippines, showed that anemonefish and anemones comprised close to

Craig S. Shuman; Gregor Hodgson; Richard F. Ambrose

2005-01-01

96

The feeding habits of three Mediterranean sea anemone species, Anemonia viridis (Forskål), Actinia equina (Linnaeus) and Cereus pedunculatus (Pennant)  

NASA Astrophysics Data System (ADS)

The feeding habits of the Mediterranean sea anemones Cereus pedunculatus, Actinia equina and Anemonia viridis were examined mainly by analysing their coelenteron contents. The three species are opportunistic omnivorous suspension feeders. Main source of food for A. viridis and C. pedunculatus are crustaceans (mainly amphipods and decapods, respectively), while for the midlittoral species A. equina, it is organic detritus. Using the same method, the temporal and spatial changes in the diet of A. viridis were examined. During the whole year, crustaceans seem to be the main source of food for A. viridis. The diet composition of this species, however, differs remarkably in space, possibly reflecting the different composition of the macrobenthic organismic assemblages in different areas. The data collected are compared with the limited bibliographical information.

Chintiroglou, Ch.; Koukouras, A.

1992-03-01

97

Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones  

PubMed Central

Marine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the transcriptomes from the adult polyp stage of two cold-water sea anemones, Bolocera tuediae and Hormathia digitata. We generated approximately 1.1 million quality-filtered sequencing reads by 454 pyrosequencing, which were assembled into approximately 120,000 contigs and 220,000 single reads. Based on annotation and gene ontology analysis we profiled the expressed mRNA transcripts according to known biological processes. As a proof-of-concept we identified polypeptide toxins with a potential blocking activity on sodium and potassium voltage-gated channels from digital transcriptome libraries. PMID:23170083

Urbarova, Ilona; Karlsen, Bård Ove; Okkenhaug, Siri; Seternes, Ole Morten; Johansen, Steinar D.; Emblem, Åse

2012-01-01

98

The 60-kDa heat shock protein (HSP60) of the sea anemone Anemonia viridis: a potential early warning system for environmental changes.  

PubMed

Expression of heat shock proteins (HSPs) is often correlated with adaptation to environmental stress. We examined the role of HSP60 (60 kDa) in acclimatization to thermal stress in the sea anemone Anemonia viridis. Using monoclonal antibodies, we identified HSP60 in sea anemones for the first time, and showed that its expression varied with changes in seawater temperature (SWT). Anemonia viridis displayed high levels of HSP60 when extreme temperatures prevailed in stressful habitats such as tidal pools. Specimens sampled from different temperature layers in the same tidal pool differed in their levels of HSP60. Specimens from subtidal zones exhibited a seasonal pattern of expression of HSP60, according to the seasonal SWT. The level of HSP60 was significantly higher in the summer (SWT, 31 degrees C) than in other seasons throughout the year. This study suggests the use of HSP60 expression as a tool for stress detection in marine invertebrates. PMID:14961344

Choresh, O; Ron, E; Loya, Y

2001-09-01

99

Vitamin B 1 thiazole derivative reduces transmembrane current through ionic channels formed by toxins from black widow spider venom and sea anemone in planar phospholipid membranes  

Microsoft Academic Search

The vitamin B1 (thiamine) structural analogue 3-decyloxycarbonylmethyl-4-methyl-5-(?-hydroxyethyl) thiazole chloride (DMHT) (0.1 mM) reversibly reduced transmembrane currents in CaCl2 and KCl solutions via ionic channels produced by latrotoxins (?-latrotoxin (?-LT) and ?-latroinsectotoxin (?-LIT)) from black widow spider venom and sea anemone toxin (RTX) in the bilayer lipid membranes (BLMs). Introduction of DMHT from the cis-side of BLM bathed in 10 mM CaCl2 inhibited

Oleg Ya. Shatursky; Tatyana M. Volkova; Olexander V. Romanenko; Nina H. Himmelreich; Eugene V. Grishin

2007-01-01

100

Exploiting the Nephrotoxic Effects of Venom from the Sea Anemone, Phyllodiscus semoni, to Create a Hemolytic Uremic Syndrome Model in the Rat  

PubMed Central

In the natural world, there are many creatures with venoms that have interesting and varied activities. Although the sea anemone, a member of the phylum Coelenterata, has venom that it uses to capture and immobilise small fishes and shrimp and for protection from predators, most sea anemones are harmless to man. However, a few species are highly toxic; some have venoms containing neurotoxins, recently suggested as potential immune-modulators for therapeutic application in immune diseases. Phyllodiscus semoni is a highly toxic sea anemone; the venom has multiple effects, including lethality, hemolysis and renal injuries. We previously reported that venom extracted from Phyllodiscus semoni induced acute glomerular endothelial injuries in rats resembling hemolytic uremic syndrome (HUS), accompanied with complement dysregulation in glomeruli and suggested that the model might be useful for analyses of pathology and development of therapeutic approaches in HUS. In this mini-review, we describe in detail the venom-induced acute renal injuries in rat and summarize how the venom of Phyllodiscus semoni could have potential as a tool for analyses of complement activation and therapeutic interventions in HUS. PMID:22851928

Mizuno, Masashi; Ito, Yasuhiko; Morgan, B. Paul

2012-01-01

101

The Evolution of MicroRNA Pathway Protein Components in Cnidaria  

PubMed Central

In the last decade, it became evident that posttranscriptional regulation of gene expression by microRNAs is a central biological process in both plants and animals. Yet, our knowledge about microRNA biogenesis and utilization in animals stems mostly from the study of Bilateria. In this study, we identified genes encoding the protein components of different parts of the microRNA pathway in Cnidaria, the likely sister phylum of Bilateria. These genes originated from three cnidarian lineages (sea anemones, stony corals, and hydras) that are separated by at least 500 My from one another. We studied the expression and phylogeny of the cnidarian homologs of Drosha and Pasha (DGCR8) that compose the microprocessor, the RNAse III enzyme Dicer and its partners, the HEN1 methyltransferase, the Argonaute protein effectors, as well as members of the GW182 protein family. We further reveal that whereas the bilaterian dicer partners Loquacious/TRBP and PACT are absent from Cnidaria, this phylum contains homologs of the double-stranded RNA-binding protein HYL1, the Dicer partner found in plants. We also identified HYL1 homologs in a sponge and a ctenophore. This finding raises questions regarding the independent evolution of the microRNA pathway in plants and animals, and together with the other results shed new light on the evolution of an important regulatory pathway. PMID:24030553

Moran, Yehu; Praher, Daniela; Fredman, David; Technau, Ulrich

2013-01-01

102

Pelagia benovici sp. nov. (Cnidaria, Scyphozoa): a new jellyfish in the Mediterranean Sea.  

PubMed

A bloom of an unknown semaestome jellyfish species was recorded in the North Adriatic Sea from September 2013 to early 2014. Morphological analysis of several specimens showed distinct differences from other known semaestome species in the Mediterranean Sea and unquestionably identified them as belonging to a new pelagiid species within genus Pelagia. The new species is morphologically distinct from P. noctiluca, currently the only recognized valid species in the genus, and from other doubtful Pelagia species recorded from other areas of the world. Molecular analyses of mitochondrial cytochrome c oxidase subunit I (COI) and nuclear 28S ribosomal DNA genes corroborate its specific distinction from P. noctiluca and other pelagiid taxa, supporting the monophyly of Pelagiidae. Thus, we describe Pelagia benovici sp. nov. Piraino, Aglieri, Scorrano & Boero. PMID:24870334

Piraino, Stefano; Aglieri, Giorgio; Martell, Luis; Mazzoldi, Carlotta; Melli, Valentina; Milisenda, Giacomo; Scorrano, Simonetta; Boero, Ferdinando

2014-01-01

103

Reproduction of the colonial hydroid Obelia geniculata (L., 1758) (Cnidaria, Hydrozoa) in the White Sea  

Microsoft Academic Search

Populations of the colonial hydroid Obelia geniculata in the White Sea reproduce asexually by frustule formation. Young medusae appear in the plankton during July and August.\\u000a The number of medusae rarely exceeds 36 per m3, and the average number varies every year from 0.4 to 10 per m3. The size of medusae is smaller than reported from other regions. The

Sergei A. Slobodov; Nickolai N. Marfenin

104

Changes in microbial communities associated with the sea anemone Anemonia viridis in a natural pH gradient.  

PubMed

Ocean acidification, resulting from rising atmospheric carbon dioxide concentrations, is a pervasive stressor that can affect many marine organisms and their symbionts. Studies which examine the host physiology and microbial communities have shown a variety of responses to the ocean acidification process. Recently, several studies were conducted based on field experiments, which take place in natural CO(2) vents, exposing the host to natural environmental conditions of varying pH. This study examines the sea anemone Anemonia viridis which is found naturally along the pH gradient in Ischia, Italy, with an aim to characterize whether exposure to pH impacts the holobiont. The physiological parameters of A. viridis (Symbiodinium density, protein, and chlorophyll a+c concentration) and its microbial community were monitored. Although reduction in pH was seen to have had an impact on composition and diversity of associated microbial communities, no significant changes were observed in A. viridis physiology, and no microbial stress indicators (i.e., pathogens, antibacterial activity, etc.) were detected. In light of these results, it appears that elevated CO(2) does not have a negative influence on A. viridis that live naturally in the site. This suggests that natural long-term exposure and dynamic diverse microbial communities may contribute to the acclimation process of the host in a changing pH environment. PMID:23011286

Meron, Dalit; Buia, Maria-Cristina; Fine, Maoz; Banin, Ehud

2013-02-01

105

The transcriptomic response to thermal stress is immediate, transient and potentiated by ultraviolet radiation in the sea anemone Anemonia viridis.  

PubMed

Among the environmental threats to coral reef health, temperature and ultraviolet increases have been proposed as major agents, although the relative contribution of each in the cnidarian/zooxanthellae symbiosis breakdown has been poorly addressed. We have investigated the transcriptomic response to thermal stress, with and without ultraviolet radiation (UVR), in the symbiotic sea anemone Anemonia viridis. Using the Oligo2K A. viridis microarray, dedicated to genes potentially involved in the symbiosis interaction, we monitored the gene expression profiles after 1, 2 and 5?days of stresses that further lead to massive losses of zooxanthellae. Each stress showed a specific gene expression profile with very little overlap. We showed that the major response to thermal stress is immediate (24?h) but returns to the baseline gene expression profile after 2?days. UVR alone has little effect but potentiates thermal stress, as a second response at 5?days was observed when the two stresses were coupled. Several pathways were highlighted, such as mesoglea loosening, cell death and calcium homeostasis and described in more details. Finally, we showed that the dermatopontin gene family, potentially involved in collagen fibrillogenesis, issued from actinarian-specific duplication events, with one member preferentially expressed in the gastroderm and specifically responding to stress. Anemonia viridis EST sequences have been deposited into GenBank dbEST ([GenBank:FK719875–FK759813]. PMID:22288383

Moya, A; Ganot, P; Furla, P; Sabourault, C

2012-03-01

106

Optimization of preservation and processing of sea anemones for microbial community analysis using molecular tools.  

PubMed

For several years, knowledge on the microbiome associated with marine invertebrates was impaired by the challenges associated with the characterization of bacterial communities. With the advent of culture independent molecular tools it is possible to gain new insights on the diversity and richness of microorganisms associated with marine invertebrates. In the present study, we evaluated if different preservation and processing methodologies (prior to DNA extraction) can affect the bacterial diversity retrieved from snakelocks anemone Anemonia viridis. Denaturing gradient gel electrophoresis (DGGE) community fingerprints were used as proxy to determine the bacterial diversity retrieved (H'). Statistical analyses indicated that preservation significantly affects H'. The best approach to preserve and process A. viridis biomass for bacterial community fingerprint analysis was flash freezing in liquid nitrogen (preservation) followed by the use of a mechanical homogenizer (process), as it consistently yielded higher H'. Alternatively, biomass samples can be processed fresh followed by cell lyses using a mechanical homogenizer or mortar &pestle. The suitability of employing these two alternative procedures was further reinforced by the quantification of the 16S rRNA gene; no significant differences were recorded when comparing these two approaches and the use of liquid nitrogen followed by processing with a mechanical homogenizer. PMID:25384534

Rocha, Joana; Coelho, Francisco J R C; Peixe, Luísa; Gomes, Newton C M; Calado, Ricardo

2014-01-01

107

Ecological and developmental dynamics of a host-parasite system involving a sea anemone and two ctenophores.  

PubMed

The lined sea anemone Edwardsiella lineata has evolved a derived parasitic life history that includes a novel body plan adapted for life inside its ctenophore hosts. Reputedly its sole host is the sea walnut, Mnemiopsis leidyi, a voracious planktivore and a seasonally abundant member of many pelagic ecosystems. However, we have observed substantially higher E. lineata prevalence in a second ctenophore species, the ctenophore predator Beroë ovata. The interplay among these 3 species has important conservation consequences as M. leidyi introductions are thought to be responsible for the severe depletion of numerous commercial fisheries in the Mediterranean basin, and both E. lineata and B. ovata have been proposed as biological controls for invasive M. leidyi. Over a 3-yr period (2004-2006), we collected 8,253 ctenophores from Woods Hole, Massachusetts, including M. leidyi, B. ovata, and a third ctenophore, Pleurobrachia pileus, and we recorded E. lineata infection frequencies, parasite load, and parasite location. We also conducted laboratory experiments to determine the likely mechanisms for parasite introduction and the effect of each host on parasite development. We observed peak E. lineata infection frequencies of 0% in P. pileus, 59% in M. leidyi, and 100% in B. ovata, suggesting that B. ovata could be an important natural host for E. lineata. However, in laboratory experiments, E. lineata larvae proved far more successful at infecting M. leidyi than B. ovata, and E. lineata parasites excised from M. leidyi exhibited greater developmental competence than parasites excised from B. ovata. Although we show that E. lineata is efficiently transferred from M. leidyi to B. ovata when the latter preys upon the former, we conclude that E. lineata larvae are not well adapted for parasitizing the latter species and that the E. lineata parasite is not well adapted for feeding in B. ovata; these developmental and ecological factors underlie the host specificity of this recently evolved parasite. PMID:18314686

Reitzel, Adam M; Sullivan, James C; Brown, Briana K; Chin, Diana W; Cira, Emily K; Edquist, Sara K; Genco, Brandon M; Joseph, Oliver C; Kaufman, Christian A; Kovitvongsa, Kathryn; Muñoz, Martha M; Negri, Tiffany L; Taffel, Jonathan R; Zuehlke, Robert T; Finnerty, John R

2007-12-01

108

Spatial gene expression quantification: a tool for analysis of in situ hybridizations in sea anemone Nematostella vectensis  

PubMed Central

Background Spatial gene expression quantification is required for modeling gene regulation in developing organisms. The fruit fly Drosophila melanogaster is the model system most widely applied for spatial gene expression analysis due to its unique embryonic properties: the shape does not change significantly during its early cleavage cycles and most genes are differentially expressed along a straight axis. This system of development is quite exceptional in the animal kingdom. In the sea anemone Nematostella vectensis the embryo changes its shape during early development; there are cell divisions and cell movement, like in most other metazoans. Nematostella is an attractive case study for spatial gene expression since its transparent body wall makes it accessible to various imaging techniques. Findings Our new quantification method produces standardized gene expression profiles from raw or annotated Nematostella in situ hybridizations by measuring the expression intensity along its cell layer. The procedure is based on digital morphologies derived from high-resolution fluorescence pictures. Additionally, complete descriptions of nonsymmetric expression patterns have been constructed by transforming the gene expression images into a three-dimensional representation. Conclusions We created a standard format for gene expression data, which enables quantitative analysis of in situ hybridizations from embryos with various shapes in different developmental stages. The obtained expression profiles are suitable as input for optimization of gene regulatory network models, and for correlation analysis of genes from dissimilar Nematostella morphologies. This approach is potentially applicable to many other metazoan model organisms and may also be suitable for processing data from three-dimensional imaging techniques. PMID:23039089

2012-01-01

109

Reproduction in two deep-sea anemones (Actiniaria); Phelliactis hertwigi and P. robusta  

NASA Astrophysics Data System (ADS)

Bathymetric distribution, abundance, substrate choice and gametogenesis have been investigated in two species of deep-sea actiniarians found in the northeast Atlantic Ocean. Phelliactis hertwigi occurs at 719-1448m in the Porcupine Seabight, usually encloses a bolus of sediment within its highly concave pedal disc (79.7%), and has abundances of up to 14.5 individuals 1000m -2. Phelliactis robusta occurs at 1600-2173m in the Porcupine Seabight, but extends deeper in the Bay of Biscay. In areas of soft sediment it is associated strongly with clinker (92.7%) and attains densities of 2.9 individuals 1000m -2. Both species are dioecious. Their sperm appear similar to those of the related intertidal Calliactis spp. Previtellogenesis and vitellogenesis of the oocytes have been defined by ultrastructural and histochemical studies. In P. hertwigi mature oocytes measure up to 180?m, and to 210?m in P. robusta. In P. hertwigi oogenesis takes 8-9 months, with spawning in October/November, whereas in the deeper-living P. robusta oogenesis occupies 15 to 19 months and spawning occurs in April/ May. Evidence is produced to suggest that these two contrasting cycles are related to the rate and seasonality of deposition of organic matter to the deep-sea floor.

van Praët, M.; Rice, A. L.; Thurston, M. H.

110

Development of Highly Selective Kv1.3-Blocking Peptides Based on the Sea Anemone Peptide ShK  

PubMed Central

ShK, from the sea anemone Stichodactyla helianthus, is a 35-residue disulfide-rich peptide that blocks the voltage-gated potassium channel Kv1.3 at ca. 10 pM and the related channel Kv1.1 at ca. 16 pM. We developed an analog of this peptide, ShK-186, which is currently in Phase 1b-2a clinical trials for the treatment of autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. While ShK-186 displays a >100-fold improvement in selectivity for Kv1.3 over Kv1.1 compared with ShK, there is considerable interest in developing peptides with an even greater selectivity ratio. In this report, we describe several variants of ShK that incorporate p-phophono-phenylalanine at the N-terminus coupled with internal substitutions at Gln16 and Met21. In addition, we also explored the combinatorial effects of these internal substitutions with an alanine extension at the C-terminus. Their selectivity was determined by patch-clamp electrophysiology on Kv1.3 and Kv1.1 channels stably expressed in mouse fibroblasts. The peptides with an alanine extension blocked Kv1.3 at low pM concentrations and exhibited up to 2250-fold selectivity for Kv1.3 over Kv1.1. Analogs that incorporates p-phosphono-phenylalanine at the N-terminus blocked Kv1.3 with IC50s in the low pM range and did not affect Kv1.1 at concentrations up to 100 nM, displaying a selectivity enhancement of >10,000-fold for Kv1.3 over Kv1.1. Other potentially important Kv channels such as Kv1.4 and Kv1.6 were only partially blocked at 100 nM concentrations of each of the ShK analogs. PMID:25603346

Pennington, Michael W.; Chang, Shih Chieh; Chauhan, Satendra; Huq, Redwan; Tajhya, Rajeev B.; Chhabra, Sandeep; Norton, Raymond S.; Beeton, Christine

2015-01-01

111

Development of Highly Selective Kv1.3-Blocking Peptides Based on the Sea Anemone Peptide ShK.  

PubMed

ShK, from the sea anemone Stichodactyla helianthus, is a 35-residue disulfide-rich peptide that blocks the voltage-gated potassium channel Kv1.3 at ca. 10 pM and the related channel Kv1.1 at ca. 16 pM. We developed an analog of this peptide, ShK-186, which is currently in Phase 1b-2a clinical trials for the treatment of autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. While ShK-186 displays a >100-fold improvement in selectivity for Kv1.3 over Kv1.1 compared with ShK, there is considerable interest in developing peptides with an even greater selectivity ratio. In this report, we describe several variants of ShK that incorporate p-phophono-phenylalanine at the N-terminus coupled with internal substitutions at Gln16 and Met21. In addition, we also explored the combinatorial effects of these internal substitutions with an alanine extension at the C-terminus. Their selectivity was determined by patch-clamp electrophysiology on Kv1.3 and Kv1.1 channels stably expressed in mouse fibroblasts. The peptides with an alanine extension blocked Kv1.3 at low pM concentrations and exhibited up to 2250-fold selectivity for Kv1.3 over Kv1.1. Analogs that incorporates p-phosphono-phenylalanine at the N-terminus blocked Kv1.3 with IC50s in the low pM range and did not affect Kv1.1 at concentrations up to 100 nM, displaying a selectivity enhancement of >10,000-fold for Kv1.3 over Kv1.1. Other potentially important Kv channels such as Kv1.4 and Kv1.6 were only partially blocked at 100 nM concentrations of each of the ShK analogs. PMID:25603346

Pennington, Michael W; Chang, Shih Chieh; Chauhan, Satendra; Huq, Redwan; Tajhya, Rajeev B; Chhabra, Sandeep; Norton, Raymond S; Beeton, Christine

2015-01-01

112

The partial purification and bioassay of a toxin present in extracts of the sea anemone, Tealia felina (L.).  

PubMed Central

1. Column chromatography with Agarose A50m followed by Sephadex G100 was used to separate a fraction (extract II) in the molecular weight range 12,000 to 14,000 daltons from saline extracts of the sea anemone, Tealia felina. 2. Extract II inhibited histamine-induced contractions of the guinea-pig ileum and produced haemolysis of human blood, effects on which bioassays were based. 3. The potency of extracts was assayed. A standard unit of activity (= AU) was defined such that 100 AU produced 90% inhibition of histamine-induced contractions of the guinea-pig ileum after 30 to 35 min exposure. 4. The relationship between activity of the extracts measured on the ileum and their haemolytic activity was studied, providing a second assay method based on the latter property. 5 Based on values from both methods of assay, the calculated yield in AU at the end of the separation procedure was 0.53 AU for each AU present in the original extract. In crude extract there were 5.0 AU/mg dry weight and 36.7 AU/mg protein, and after separation (extract II) there were 11.2 AU/mg dry weight and 312.2 AU/mg protein. 6 The acute LD50 values determined in mice (i.v.) were: for crude extract 124 mg/kg for extract I, 76 mg/kg and for extract II, 69 mg/kg. 7 Extract II (0.18 to 0.72 AU/ml) produced a slowly developing contraction of guinea-pig ileum. Indomethacin (2.8 x 10(-5) M) substantially reduced this response. 8 Extract II (0.03 AU/ml) reduced the contractile response of the guinea-pig ileum to acetylcholine by 39 +/- 8%, n = 6, and the response to histamine by 26 +/- 6.6%, n = 6. The response to 5-hydroxytryptamine (5-HT) was not reduced by 0.08 AU/ml of extract II, a concentration that actually increased the contractile response to KC1 by 32 +/- 11.2% n = 7. 9 It is proposed that for future work on the extract a new AU should be used. This AU is defined such that 50 AU produce 50% inhibition of histamine-induced contractions of the guinea-pig ileum after 30 to 35 min exposure. PMID:6111370

Aldeen, S. I.; Elliott, R. C.; Sheardown, M.

1981-01-01

113

The sea anemone Bunodosoma caissarum toxin BcIII modulates the sodium current kinetics of rat dorsal root ganglia neurons and is displaced in a voltage-dependent manner.  

PubMed

Sea anemone toxins bind to site 3 of the sodium channels, which is partially formed by the extracellular linker connecting S3 and S4 segments of domain IV, slowing down the inactivation process. In this work we have characterized the actions of BcIII, a sea anemone polypeptide toxin isolated from Bunodosoma caissarum, on neuronal sodium currents using the patch clamp technique. Neurons of the dorsal root ganglia of Wistar rats (P5-9) in primary culture were used for this study (n=65). The main effects of BcIII were a concentration-dependent increase in the sodium current inactivation time course (IC(50)=2.8 microM) as well as an increase in the current peak amplitude. BcIII did not modify the voltage at which 50% of the channels are activated or inactivated, nor the reversal potential of sodium current. BcIII shows a voltage-dependent action. A progressive acceleration of sodium current fast inactivation with longer conditioning pulses was observed, which was steeper as more depolarizing were the prepulses. The same was observed for other two anemone toxins (CgNa, from Condylactis gigantea and ATX-II, from Anemonia viridis). These results suggest that the binding affinity of sea anemone toxins may be reduced in a voltage-dependent manner, as has been described for alpha-scorpion toxins. PMID:20015459

Salceda, Emilio; López, Omar; Zaharenko, André J; Garateix, Anoland; Soto, Enrique

2010-03-01

114

Structure-function relationships of the major neurotoxin from the sea anemone Stichodactyla helianthus with a new sodium channel receptor site  

SciTech Connect

We have determined that ShN I, a 48-residue type 2 sea anemone toxin, delays the inactivation of the Na channel in lobster olfactory somas. The receptor for ShN I was identified in vesicle preparations of neuronal tissues from both crustaceans and mammals; however, the K{sub D} values for the former is more than 1,000 fold lower for the later. The binding of ({sup 125}I)-ShN I to this receptor was determined to be unaffected by Anemonia sulcata II, depolarization of the membrane, or veratridine. ShN I was unable to displace ({sup 125}I)-Androctonus austrialis Hector II, whereas unlabeled AaH II and As II displaced the labeled scorpion toxin from rat brain synaptosomes. This is the first characterization of a new Na channel receptor site which specifically binds type 2 anemone toxins. To study the interactions that specific amino acid residues of ShN I have with this receptor, we developed a strategy using solid phase peptide synthesis. Prior to the synthesis of analogs to ShN I, we assembled the native ShN I sequence and reoxidized the three intramolecular disulfide bonds. Chemical, physical, and pharmacological characterization of the purified synthetic ShN I showed it to be indistinguishable from the natural toxin.

Pennington, M.W.

1988-01-01

115

Detrimental effects of host anemone bleaching on anemonefish populations  

NASA Astrophysics Data System (ADS)

Coral bleaching and related reef degradation have caused significant declines in the abundance of reef-associated fishes. Most attention on the effects of bleaching has focused on corals, but bleaching is also prevalent in other cnidarians, including sea anemones. The consequences of anemone bleaching are unknown, and the demographic effects of bleaching on associated fish recruitment, survival, and reproduction are poorly understood. We examined the effect of habitat degradation including host anemone bleaching on fish abundance, egg production, and recruitment of the panda anemonefish ( Amphiprion polymnus) near Port Moresby, Papua New Guinea. Following a high-temperature anomaly in shallow waters of the region, most shallow anemones to a depth of 6 m (approximately 35% of all the anemones in this area) were severely bleached. Anemone mortality was low but bleached anemones underwent a ~34% reduction in body size. Total numbers of A. polymnus were not affected by bleaching and reduction in shelter area. While egg production of females living in bleached anemones was reduced by ~38% in 2009 compared to 2008, egg production of females on unbleached anemones did not differ significantly between years. Total recruitment in 2009 was much lower than in 2008. However, we found no evidence of recruiting larvae avoiding bleached anemones at settlement suggesting that other factors or different chemical cues were more important in determining recruitment than habitat quality. These results provide the first field evidence of detrimental effects of climate-induced bleaching and habitat degradation on reproduction and recruitment of anemonefish.

Saenz-Agudelo, P.; Jones, G. P.; Thorrold, S. R.; Planes, S.

2011-06-01

116

Molecular analysis of the sea anemone toxin Av3 reveals selectivity to insects and demonstrates the heterogeneity of receptor site-3 on voltage-gated Na+ channels  

PubMed Central

Av3 is a short peptide toxin from the sea anemone Anemonia viridis shown to be active on crustaceans and inactive on mammals. It inhibits inactivation of Navs (voltage-gated Na+ channels) like the structurally dissimilar scorpion ?-toxins and type I sea anemone toxins that bind to receptor site-3. To examine the potency and mode of interaction of Av3 with insect Navs, we established a system for its expression, mutagenized it throughout, and analysed it in toxicity, binding and electrophysiological assays. The recombinant Av3 was found to be highly toxic to blowfly larvae (ED50=2.65±0.46 pmol/100 mg), to compete well with the site-3 toxin Lqh?IT (from the scorpion Leiurus quinquestriatus) on binding to cockroach neuronal membranes (Ki=21.4±7.1 nM), and to inhibit the inactivation of Drosophila melanogaster channel, DmNav1, but not that of mammalian Navs expressed in Xenopus oocytes. Moreover, like other site-3 toxins, the activity of Av3 was synergically enhanced by ligands of receptor site-4 (e.g. scorpion ?-toxins). The bioactive surface of Av3 was found to consist mainly of aromatic residues and did not resemble any of the bioactive surfaces of other site-3 toxins. These analyses have portrayed a toxin that might interact with receptor site-3 in a different fashion compared with other ligands of this site. This assumption was corroborated by a D1701R mutation in DmNav1, which has been shown to abolish the activity of all other site-3 ligands, except Av3. All in all, the present study provides further evidence for the heterogeneity of receptor site-3, and raises Av3 as a unique model for design of selective anti-insect compounds. PMID:17492942

Moran, Yehu; Kahn, Roy; Cohen, Lior; Gur, Maya; Karbat, Izhar; Gordon, Dalia; Gurevitz, Michael

2007-01-01

117

Molecular analysis of the sea anemone toxin Av3 reveals selectivity to insects and demonstrates the heterogeneity of receptor site-3 on voltage-gated Na+ channels.  

PubMed

Av3 is a short peptide toxin from the sea anemone Anemonia viridis shown to be active on crustaceans and inactive on mammals. It inhibits inactivation of Na(v)s (voltage-gated Na+ channels) like the structurally dissimilar scorpion alpha-toxins and type I sea anemone toxins that bind to receptor site-3. To examine the potency and mode of interaction of Av3 with insect Na(v)s, we established a system for its expression, mutagenized it throughout, and analysed it in toxicity, binding and electrophysiological assays. The recombinant Av3 was found to be highly toxic to blowfly larvae (ED50=2.65+/-0.46 pmol/100 mg), to compete well with the site-3 toxin LqhalphaIT (from the scorpion Leiurus quinquestriatus) on binding to cockroach neuronal membranes (K(i)=21.4+/-7.1 nM), and to inhibit the inactivation of Drosophila melanogaster channel, DmNa(v)1, but not that of mammalian Na(v)s expressed in Xenopus oocytes. Moreover, like other site-3 toxins, the activity of Av3 was synergically enhanced by ligands of receptor site-4 (e.g. scorpion beta-toxins). The bioactive surface of Av3 was found to consist mainly of aromatic residues and did not resemble any of the bioactive surfaces of other site-3 toxins. These analyses have portrayed a toxin that might interact with receptor site-3 in a different fashion compared with other ligands of this site. This assumption was corroborated by a D1701R mutation in DmNa(v)1, which has been shown to abolish the activity of all other site-3 ligands, except Av3. All in all, the present study provides further evidence for the heterogeneity of receptor site-3, and raises Av3 as a unique model for design of selective anti-insect compounds. PMID:17492942

Moran, Yehu; Kahn, Roy; Cohen, Lior; Gur, Maya; Karbat, Izhar; Gordon, Dalia; Gurevitz, Michael

2007-08-15

118

Daly, M. and D.G. Fautin. 2004. Anthopleura mariscali, a new species of sea anemone (Cnidaria: Anthozoa: Actiniaria) from the Galapagos Islands. Zootaxa 416:1-8.  

E-print Network

. Sebens, K.P. & Paine, R.T. (1979) Biogeography of anthozoans along the west coast of South America: habi- tat, disturbance, and prey availability. Proceedings of the International Symposium on Marine Biogeogra- phy and Evolution in the Southern... A. nigrescens Tentacle Spirocyst (A) 4/4 56 14.0–22.0 x 1.8–3.3 10.3–28.8 x 1.8–3.8 Basitrich (B) 4/4 71 (8.9) 12.3–19.5 x (1.4) 1.7–3.1 (3.5) 12.0–14.4 x 1.2 Basitrich 15.6–24.0 x 1.2–2.4 Acrorhagus Holotrich I (H) 4/4 48 35.5–59.7 x 2.7–5.2 30...

Daly, Marymegan; Fautin, Daphne G.

2004-01-30

119

A new species, *Adamsia obvolva* (Cnidaria: Anthozoa: Actiniaria), from the Gulf of Mexico, and a discussion of the taxonomy of carcinoecium-forming sea anemones  

E-print Network

rather than the number of sterile mesenteries to distinguish between hormathiid species that live attached to gas- tropod shells inhabited by hermit crabs facilitates identi?cation, especially of infertile specimens. We interpret a second cycle... was supported by NSF grant DEB 99-78106 (PEET: Partnerships for Enhancing Expertise in Taxonomy) to D. G. Fautin, NSF grant OCE 00-03970 (NOPP: National Oceano- graphic Partnership Program) to D.G. Fautin and R. W. Buddemeier, and an NSF Graduate Stu- dent...

Daly, Marymegan; Ardelean, Adorian; Cha, Ha-Rim; Campbell, Andrew C.; Fautin, Daphne G.

2004-01-01

120

Increasing pCO2 correlates with low concentrations of intracellular dimethylsulfoniopropionate in the sea anemone Anemonia viridis  

PubMed Central

Marine anthozoans maintain a mutualistic symbiosis with dinoflagellates that are prolific producers of the algal secondary metabolite dimethylsulfoniopropionate (DMSP), the precursor of the climate-cooling trace gas dimethyl sulfide (DMS). Surprisingly, little is known about the physiological role of DMSP in anthozoans and the environmental factors that regulate its production. Here, we assessed the potential functional role of DMSP as an antioxidant and determined how future increases in seawater pCO2 may affect DMSP concentrations in the anemone Anemonia viridis along a natural pCO2 gradient at the island of Vulcano, Italy. There was no significant difference in zooxanthellae genotype and characteristics (density of zooxanthellae, and chlorophyll a) as well as protein concentrations between anemones from three stations along the gradient, V1 (3232 ?atm CO2), V2 (682 ?atm) and control (463 ?atm), which indicated that A.?viridis can acclimate to various seawater pCO2. In contrast, DMSP concentrations in anemones from stations V1 (33.23?±?8.30 fmol cell?1) and V2 (34.78?±?8.69 fmol cell?1) were about 35% lower than concentrations in tentacles from the control station (51.85?±?12.96 fmol cell?1). Furthermore, low tissue concentrations of DMSP coincided with low activities of the antioxidant enzyme superoxide dismutase (SOD). Superoxide dismutase activity for both host (7.84?±?1.37?U·mg?1 protein) and zooxanthellae (2.84?±?0.41?U·mg?1 protein) at V1 was 40% lower than at the control station (host: 13.19?±?1.42; zooxanthellae: 4.72?±?0.57?U·mg?1 protein). Our results provide insight into coastal DMSP production under predicted environmental change and support the function of DMSP as an antioxidant in symbiotic anthozoans. PMID:24634728

Borell, Esther M; Steinke, Michael; Horwitz, Rael; Fine, Maoz

2014-01-01

121

Increasing pCO2 correlates with low concentrations of intracellular dimethylsulfoniopropionate in the sea anemone Anemonia viridis.  

PubMed

Marine anthozoans maintain a mutualistic symbiosis with dinoflagellates that are prolific producers of the algal secondary metabolite dimethylsulfoniopropionate (DMSP), the precursor of the climate-cooling trace gas dimethyl sulfide (DMS). Surprisingly, little is known about the physiological role of DMSP in anthozoans and the environmental factors that regulate its production. Here, we assessed the potential functional role of DMSP as an antioxidant and determined how future increases in seawater pCO2 may affect DMSP concentrations in the anemone Anemonia viridis along a natural pCO2 gradient at the island of Vulcano, Italy. There was no significant difference in zooxanthellae genotype and characteristics (density of zooxanthellae, and chlorophyll a) as well as protein concentrations between anemones from three stations along the gradient, V1 (3232 ?atm CO2), V2 (682 ?atm) and control (463 ?atm), which indicated that A.?viridis can acclimate to various seawater pCO2. In contrast, DMSP concentrations in anemones from stations V1 (33.23?±?8.30 fmol cell(-1)) and V2 (34.78?±?8.69 fmol cell(-1)) were about 35% lower than concentrations in tentacles from the control station (51.85?±?12.96 fmol cell(-1)). Furthermore, low tissue concentrations of DMSP coincided with low activities of the antioxidant enzyme superoxide dismutase (SOD). Superoxide dismutase activity for both host (7.84?±?1.37?U·mg(-1) protein) and zooxanthellae (2.84?±?0.41?U·mg(-1) protein) at V1 was 40% lower than at the control station (host: 13.19?±?1.42; zooxanthellae: 4.72?±?0.57?U·mg(-1) protein). Our results provide insight into coastal DMSP production under predicted environmental change and support the function of DMSP as an antioxidant in symbiotic anthozoans. PMID:24634728

Borell, Esther M; Steinke, Michael; Horwitz, Rael; Fine, Maoz

2014-02-01

122

Nitric oxide mediates coral bleaching through an apoptotic-like cell death pathway: evidence from a model sea anemone-dinoflagellate symbiosis.  

PubMed

Coral bleaching (involving the loss of symbiotic algae from the cnidarian host) is a major threat to coral reefs and appears to be mediated at the cellular level by nitric oxide (NO). In this study, we examined the specific role of NO in bleaching using the sea anemone Aiptasia pulchella, a model system for the study of corals. Exposure of A. pulchella to high-temperature shock (26-33°C over <1 h) or an NO donor (S-nitrosoglutathione) resulted in significant increases in host caspase-like enzyme activity. These responses were reflected in the intensities of bleaching, which were significantly higher in heat- or NO-treated specimens than in controls maintained in seawater at 26°C. Notably, the inhibition of caspase-like activity prevented bleaching even in the presence of an NO donor or at elevated temperature. The additional use of an NO scavenger controlled for effects mediated by agents other than NO. We also exposed A. pulchella to a more ecologically relevant treatment (an increase from 26 to 33°C over 6-7 d). Again, host NO synthesis correlated with the activation of caspase-like enzyme activity. Therefore, we conclude that NO's involvement in cnidarian bleaching arises through the regulation of host apoptotic pathways. PMID:23934282

Hawkins, Thomas D; Bradley, Benjamin J; Davy, Simon K

2013-12-01

123

A Protein Toxin from the Sea Anemone Phyllodiscus semoni Targets the Kidney and Causes a Severe Renal Injury with Predominant Glomerular Endothelial Damage  

PubMed Central

Envenomation by the sea anemone Phyllodiscus semoni causes fulminant dermatitis and, rarely, acute renal failure in humans. Here, we investigated whether the venom extracted from the nematocysts (PsTX-T) was nephrotoxic when administered intravenously in rats and whether PsTX-T induced activation of the complement system. Although small dose of PsTX-T induced acute tubular necrosis in rats resembling pathology seen in patients, kidneys displayed glomerular injury with glomerular endothelial damage, thrombus formation, mesangiolysis, and partial rupture of glomerular basement membrane, accompanied by severe tubular necrosis at 24 hours after administration of 0.03 mg of PsTX-T per animal, similar to the glomerular findings typical of severe hemolytic uremic syndrome. The early stage injury was accompanied by specific PsTX-T binding, massive complement C3b, and membrane attack complex deposition in glomeruli in the regions of injury and decreased glomerular expression of complement regulators. A pathogenic role for complement was confirmed by demonstrating that systemic complement inhibition reduced renal injury. The isolated nephrotoxic component, a 115-kd protein toxin (PsTX-115), was shown to cause identical renal pathology. The demonstration that PsTX-T and PsTX-115 were highly nephrotoxic acting via induction of complement activation suggests that inhibition of complement might be used to prevent acute renal damage following envenomation by P. semoni. PMID:17600120

Mizuno, Masashi; Nozaki, Masatoshi; Morine, Nobuya; Suzuki, Norihiko; Nishikawa, Kazuhiro; Morgan, B. Paul; Matsuo, Seiichi

2007-01-01

124

Is boldness a resource-holding potential trait? Fighting prowess and changes in startle response in the sea anemone, Actinia equina  

PubMed Central

Contest theory predicts the evolution of a stable mixture of different strategies for fighting. Here, we investigate the possibility that stable between-individual differences in startle-response durations influence fighting ability or ‘resource-holding potential’ (RHP) in the beadlet sea anemone, Actinia equina. Both winners and losers showed significant repeatability of pre-fight startle-response durations but mean pre-fight startle-response durations were greater for eventual losers than for eventual winners, indicating that RHP varies with boldness. In particular, individuals with short startle responses inflicted more attacks on their opponent. Both repeatability and mean-level responses were changed by the experience of fighting, and these changes varied with outcome. In losers, repeatability was disrupted to a greater extent and the mean startle-response durations were subject to a greater increase than in winners. Thus, following a fight, this behavioural correlate of RHP behaves in a way similar to post-fight changes in physiological status, which can also vary between winners and losers. Understanding the links between aggression and boldness therefore has the potential to enhance our understanding of both the evolution of animal personality and the ‘winner and loser effects’ of post-fight changes in RHP. PMID:22171080

Rudin, Fabian S.; Briffa, Mark

2012-01-01

125

Cyclisation increases the stability of the sea anemone peptide APETx2 but decreases its activity at acid-sensing ion channel 3.  

PubMed

APETx2 is a peptide isolated from the sea anemone Anthopleura elegantissima. It is the most potent and selective inhibitor of acid-sensing ion channel 3 (ASIC3) and it is currently in preclinical studies as a novel analgesic for the treatment of chronic inflammatory pain. As a peptide it faces many challenges in the drug development process, including the potential lack of stability often associated with therapeutic peptides. In this study we determined the susceptibility of wild-type APETx2 to trypsin and pepsin and tested the applicability of backbone cyclisation as a strategy to improve its resistance to enzymatic degradation. Cyclisation with either a six-, seven- or eight-residue linker vastly improved the protease resistance of APETx2 but substantially decreased its potency against ASIC3. This suggests that either the N- or C-terminus of APETx2 is involved in its interaction with the channel, which we confirmed by making N- and C-terminal truncations. Truncation of either terminus, but especially the N-terminus, has detrimental effects on the ability of APETx2 to inhibit ASIC3. The current work indicates that cyclisation is unlikely to be a suitable strategy for stabilising APETx2, unless linkers can be engineered that do not interfere with binding to ASIC3. PMID:22851922

Jensen, Jonas E; Mobli, Mehdi; Brust, Andreas; Alewood, Paul F; King, Glenn F; Rash, Lachlan D

2012-07-01

126

Cyclisation Increases the Stability of the Sea Anemone Peptide APETx2 but Decreases Its Activity at Acid-Sensing Ion Channel 3  

PubMed Central

APETx2 is a peptide isolated from the sea anemone Anthopleura elegantissima. It is the most potent and selective inhibitor of acid-sensing ion channel 3 (ASIC3) and it is currently in preclinical studies as a novel analgesic for the treatment of chronic inflammatory pain. As a peptide it faces many challenges in the drug development process, including the potential lack of stability often associated with therapeutic peptides. In this study we determined the susceptibility of wild-type APETx2 to trypsin and pepsin and tested the applicability of backbone cyclisation as a strategy to improve its resistance to enzymatic degradation. Cyclisation with either a six-, seven- or eight-residue linker vastly improved the protease resistance of APETx2 but substantially decreased its potency against ASIC3. This suggests that either the N- or C-terminus of APETx2 is involved in its interaction with the channel, which we confirmed by making N- and C-terminal truncations. Truncation of either terminus, but especially the N-terminus, has detrimental effects on the ability of APETx2 to inhibit ASIC3. The current work indicates that cyclisation is unlikely to be a suitable strategy for stabilising APETx2, unless linkers can be engineered that do not interfere with binding to ASIC3. PMID:22851922

Jensen, Jonas E.; Mobli, Mehdi; Brust, Andreas; Alewood, Paul F.; King, Glenn F.; Rash, Lachlan D.

2012-01-01

127

Improved purification and enzymatic properties of a mixture of Sticholysin I and II: isotoxins with hemolytic and phospholipase A(2) activities from the sea anemone Stichodactyla helianthus.  

PubMed

Sticholysin I and Sticholysin II (StI and StII) are two potent hemolysins which form pores in natural and model membranes at nanomolar concentrations. These proteins were purified from the aqueous extract of the sea anemone Stichodactyla helianthus, Ellis 1768, by gel filtration and ionic exchange chromatography. This procedure rendered StI and StII with high purity (purification factors: 36 and 50, respectively) but a low yield of hemolytic activity, HA (<3%). Additionally, these toxins exhibited very low phospholipase activity (10(-3)U/mg of protein). In this work, a mixture StI-StII was obtained (yield >95%, with an increase in specific activity: 14 times) from the animal extract using an oxidized phospholipid-based affinity chromatographic matrix binding phospholipases. Cytolysin identification in the mixture was performed by immunoblotting and N-terminal sequence analyses. Phospholipase A2 (PLA2) activity of StI-StII was relatively high (1.85U/mg) and dependent of Ca(2+). The activity resulted optimum when was measured with the mostly unsaturated soybean phosphatidylcholine (PC), when compared to the less unsaturated egg PC or completely saturated dipalmitoyl PC, in the presence of 40mM Ca(2+) at pH 8.0. This Ca(2+) concentration did not exert any effect on binding of StI-StII with soybean PC monolayers. Then, PLA2 activity seems not be required to binding to membranes. PMID:24326193

del Monte-Martínez, Alberto; González-Bacerio, Jorge; Romero, Lázara; Aragón, Carlos; Martínez, Diana; de Los Á Chávez, María; Álvarez, Carlos; Lanio, María E; Guisán, José M; Díaz, Joaquín

2014-03-01

128

Production of a reference transcriptome and transcriptomic database (EdwardsiellaBase) for the lined sea anemone, Edwardsiella lineata, a parasitic cnidarian  

PubMed Central

Background The lined sea anemone Edwardsiella lineata is an informative model system for evolutionary-developmental studies of parasitism. In this species, it is possible to compare alternate developmental pathways leading from a larva to either a free-living polyp or a vermiform parasite that inhabits the mesoglea of a ctenophore host. Additionally, E. lineata is confamilial with the model cnidarian Nematostella vectensis, providing an opportunity for comparative genomic, molecular and organismal studies. Description We generated a reference transcriptome for E. lineata via high-throughput sequencing of RNA isolated from five developmental stages (parasite; parasite-to-larva transition; larva; larva-to-adult transition; adult). The transcriptome comprises 90,440 contigs assembled from >15 billion nucleotides of DNA sequence. Using a molecular clock approach, we estimated the divergence between E. lineata and N. vectensis at 215–364 million years ago. Based on gene ontology and metabolic pathway analyses and gene family surveys (bHLH-PAS, deiodinases, Fox genes, LIM homeodomains, minicollagens, nuclear receptors, Sox genes, and Wnts), the transcriptome of E. lineata is comparable in depth and completeness to N. vectensis. Analyses of protein motifs and revealed extensive conservation between the proteins of these two edwardsiid anemones, although we show the NF-?B protein of E. lineata reflects the ancestral structure, while the NF-?B protein of N. vectensis has undergone a split that separates the DNA-binding domain from the inhibitory domain. All contigs have been deposited in a public database (EdwardsiellaBase), where they may be searched according to contig ID, gene ontology, protein family motif (Pfam), enzyme commission number, and BLAST. The alignment of the raw reads to the contigs can also be visualized via JBrowse. Conclusions The transcriptomic data and database described here provide a platform for studying the evolutionary developmental genomics of a derived parasitic life cycle. In addition, these data from E. lineata will aid in the interpretation of evolutionary novelties in gene sequence or structure that have been reported for the model cnidarian N. vectensis (e.g., the split NF-?B locus). Finally, we include custom computational tools to facilitate the annotation of a transcriptome based on high-throughput sequencing data obtained from a “non-model system.” PMID:24467778

2014-01-01

129

Pachyseris inattesa sp. n. (Cnidaria, Anthozoa, Scleractinia): a new reef coral species from the Red Sea and its phylogenetic relationships  

PubMed Central

Abstract A new scleractinian coral species, Pachyseris inattesa sp. n., is described from the Red Sea. Despite a superficial resemblance with some species in the agariciid genus Leptoseris with which it has been previously confused, P. inattesa sp. n. has micro-morphological characters typical of the genus Pachyseris. This genus, once part of the Agariciidae, is comprised of five extant species and is widely distributed throughout the tropical Indo-Pacific. It is currently incertae sedis as a result of recent molecular analysis and appears to be closely related to the Euphylliidae. A molecular phylogenetic reconstruction including P. inattesa sp. n., the genus type species P. rugosa, and P. speciosa, all present in the Red Sea, was performed using the mitochondrial intergenic spacer between COI and 16S-rRNA. The results confirm that P. inattesa sp. n. is a monophyletic lineage closely related to the other Pachyseris species examined. PMID:25152672

Terraneo, Tullia I.; Berumen, Michael L.; Arrigoni, Roberto; Waheed, Zarinah; Bouwmeester, Jessica; Caragnano, Annalisa; Stefani, Fabrizio; Benzoni, Francesca

2014-01-01

130

[The thiazole derivative reduces transmembrane currents via ionic channels formed by alpha-latrotoxin and sea anemone toxin in the bilayer lipid membranes].  

PubMed

It was shown that the thiazole derivative 3-decyloxycarbonylmethyl-4-methyl- 5-(2-hydroxyethyl)thiazole chloride (DMHT) (0.1 mM) reversibly reduced the transmembrane current in solutions of 10 mM CaCl2 and 100 mM KCl via ionic channels produced by alpha-latrotoxin from black widow spider (alpha-LT) and sea anemone toxin (RTX) in the bilayer lipid membranes (BLM). Introduction of DMHT from the cis-side of BLM inhibited transmembrane current by 31.6 +/- 3% and by 61.8 +/- 3% from the trans-side of BLM for alpha-LT channels. Application of DMHT to the cis-side BLM decreased the inward current through the RTX channels by 50 +/- 5%. Addition of Cd(2+) (0.1 mM) to the cis- or trans-side of a membrane after the DMHT induced depression of transmembrane current across the alpha-LT channels caused its further decrease by 85 +/- 5% that coincides completely with the intensity of Cd(2+)-inhibition in the control experiments without DMHT. These data suggest that DMHT may exert its inhibitory action on alpha-LT channels without considerable influence on the ionogenic groups inside the channel cavity. The comparative analysis of effective radii measured for alpha-LT and RTX channels on the cis- (0.9 nm and 0.55 nm, respectively) and the trans-side of BLM (< 0.467 nm for alpha-LT) allowed to propose the blocking action of DMHT for alpha-LT and RTX channels to result from direct penetration into the channel, achieved due to similar hydrodynamic size of blocking molecules and the size of toxin pores. PMID:16568603

Shaturski?, O Ia; Romanenko, A V

2005-01-01

131

Phyla- and Subtype-Selectivity of CgNa, a Na+ Channel Toxin from the Venom of the Giant Caribbean Sea Anemone Condylactis Gigantea  

PubMed Central

Because of their prominent role in electro-excitability, voltage-gated sodium (NaV) channels have become the foremost important target of animal toxins. These toxins have developed the ability to discriminate between closely related NaV subtypes, making them powerful tools to study NaV channel function and structure. CgNa is a 47-amino acid residue type I toxin isolated from the venom of the Giant Caribbean Sea Anemone Condylactis gigantea. Previous studies showed that this toxin slows the fast inactivation of tetrodotoxin-sensitive NaV currents in rat dorsal root ganglion neurons. To illuminate the underlying NaV subtype-selectivity pattern, we have assayed the effects of CgNa on a broad range of mammalian isoforms (NaV1.2–NaV1.8) expressed in Xenopus oocytes. This study demonstrates that CgNa selectively slows the fast inactivation of rNaV1.3/?1, mNaV1.6/?1 and, to a lesser extent, hNaV1.5/?1, while the other mammalian isoforms remain unaffected. Importantly, CgNa was also examined on the insect sodium channel DmNaV1/tipE, revealing a clear phyla-selectivity in the efficacious actions of the toxin. CgNa strongly inhibits the inactivation of the insect NaV channel, resulting in a dramatic increase in peak current amplitude and complete removal of fast and steady-state inactivation. Together with the previously determined solution structure, the subtype-selective effects revealed in this study make of CgNa an interesting pharmacological probe to investigate the functional role of specific NaV channel subtypes. Moreover, further structural studies could provide important information on the molecular mechanism of NaV channel inactivation. PMID:21833172

Billen, Bert; Debaveye, Sarah; Béress, Lászlo; Tytgat, Jan

2010-01-01

132

The specificity of Av3 sea anemone toxin for arthropods is determined at linker DI/SS2-S6 in the pore module of target sodium channels.  

PubMed

Av3 is a peptide neurotoxin from the sea anemone Anemonia viridis that shows specificity for arthropod voltage-gated sodium channels (Navs). Interestingly, Av3 competes with a scorpion ?-toxin on binding to insect Navs and similarly inhibits the inactivation process, and thus has been classified as 'receptor site-3 toxin', although the two peptides are structurally unrelated. This raises questions as to commonalities and differences in the way both toxins interact with Navs. Recently, site-3 was partly resolved for scorpion ?-toxins highlighting S1-S2 and S3-S4 external linkers at the DIV voltage-sensor module and the juxtaposed external linkers at the DI pore module. To uncover channel determinants involved in Av3 specificity for arthropods, the toxin was examined on channel chimaeras constructed with the external linkers of the mammalian brain Nav1.2a, which is insensitive to Av3, in the background of the Drosophila DmNav1. This approach highlighted the role of linker DI/SS2-S6, adjacent to the channel pore, in determining Av3 specificity. Point mutagenesis at DI/SS2-S6 accompanied by functional assays highlighted Trp404 and His405 as a putative point of Av3 interaction with DmNav1. His405 conservation in arthropod Navs compared with tyrosine in vertebrate Navs may represent an ancient substitution that explains the contemporary selectivity of Av3. Trp404 and His405 localization near the membrane surface and the hydrophobic bioactive surface of Av3 suggest that the toxin possibly binds at a cleft by DI/S6. A partial overlap in receptor site-3 of both toxins nearby DI/S6 may explain their binding competition capabilities. PMID:25055135

Gur Barzilai, Maya; Kahn, Roy; Regev, Noa; Gordon, Dalia; Moran, Yehu; Gurevitz, Michael

2014-10-15

133

Cooperativity in the two-domain arginine kinase from the sea anemone Anthopleura japonicus. II. Evidence from site-directed mutagenesis studies.  

PubMed

The arginine kinase (AK) from the sea anemone Anthopleura japonicus has an unusual two-domain structure (contiguous dimer; denoted by D1-D2). In a previous report, we suggested cooperativity in the contiguous dimer, which may be a result of domain-domain interactions, using MBP-fused enzymes. To further understand this observation, we inserted six-Lys residues into the linker region of the two-domain AK (D1-K6-D2 mutant) using His-tagged enzyme. The dissociation constants, K(a) and K(ia), of the mutant were similar to those of the wild-type enzyme but the catalytic constant, k(cat), was decreased to 28% that of the wild-type, indicating that some of the domain-domain interactions are lost due to the six-Lys insertion. Y68 plays a major role in arginine binding in the catalytic pocket in Limulus AK, and introduction of mutation at the Y68 position virtually abolishes catalytic activity. Thus, the constructed D1(Y68G)-D2 and D1-D2(Y68G) mutants mimic the D1(inactive)-D2(active) and D1(active)-D2(inactive) enzymes, respectively. The k(cat) values of both Y68 mutants were decreased to 13-18% that of the wild-type enzyme, which is much less than the 50% level of the two-domain enzyme. Thus, it is clear that substrate-binding to both domains is necessary for full expression of activity. In other words, substrate-binding appears to act as the trigger of the functional cooperativity in two-domain AK. PMID:20434482

Tada, Hiroshi; Suzuki, Tomohiko

2010-08-01

134

Giant Caribbean Anemone (Condylactis gigantea)  

USGS Multimedia Gallery

Condylactis gigantea fluourescing on rocks. The anemones are in a tank illuminated by ultraviolet (black) light. The anemones absorb the invisible ultraviolet light and reflect it back as visible bright green light....

135

Phylum Cnidaria Origin of Diversity  

E-print Network

found on the hermit crab shell. How has so much diversity been possible in the Phylum Cnidaria 3. Coral all polyps through stolons Hydractinia #12;3 Metamorphosis is triggered by unknown cues from bacteria

Cochran-Stafira, D. Liane

136

Vitamin B1 thiazole derivative reduces transmembrane current through ionic channels formed by toxins from black widow spider venom and sea anemone in planar phospholipid membranes.  

PubMed

The vitamin B1 (thiamine) structural analogue 3-decyloxycarbonylmethyl-4-methyl-5-(beta-hydroxyethyl) thiazole chloride (DMHT) (0.1 mM) reversibly reduced transmembrane currents in CaCl2 and KCl solutions via ionic channels produced by latrotoxins (alpha-latrotoxin (alpha-LT) and alpha-latroinsectotoxin (alpha-LIT)) from black widow spider venom and sea anemone toxin (RTX) in the bilayer lipid membranes (BLMs). Introduction of DMHT from the cis-side of BLM bathed in 10 mM CaCl2 inhibited transmembrane current by 31.6+/-3% and by 61.8+/-3% from the trans-side of BLM for alpha-LT channels. Application of DMHT in the solution of 10 mM CaCl2 to the cis-side of BLM decreased the current through the alpha-LIT and RTX channels by 52+/-4% and 50+/-5%, respectively. Addition of Cd2+ (1 mM) to the cis- or trans-side of the membrane after the DMHT-induced depression of Ca2+-current across the alpha-LT channels caused its further decrease by 85+/-5% that coincides favorably with the intensity of Cd2+ blocking in control experiments without DMHT. These data suggest that DMHT inhibiting is not specific for latrotoxin channels only and DMHT may exert its action on alpha-LT channels without considerable influence on the ionogenic groups of Ca2+-selective site inside the channel cavity. The binding kinetics of DMHT with the alpha-LT channel shows no cooperativity and allows to expect that the DMHT binding site of the toxin is formed by one ionogenic group as the slopes of inhibition rate determined in log-log coordinates are 1.25 on the trans-side and 0.68 on the cis-side. Similar pK of binding (5.4 on the trans-side and 5.7 on the cis-side) also suggest that DMHT may interact with the same high affinity site of alpha-LT channel on either side of the BLM. The comparative analysis of effective radii measured for alpha-LT, alpha-LIT and RTX channels on the cis-side (0.9 nm, 0.53 nm and 0.55 nm, correspondingly) and for alpha-LT channel on the trans-side (0.28+/-0.18 nm) with the intensity of DMHT inhibitory action obtained on these channels allowed to conclude that the potency of DMHT inhibition increased on toxin pores of smaller lumen. PMID:17150177

Shatursky, Oleg Ya; Volkova, Tatyana M; Romanenko, Olexander V; Himmelreich, Nina H; Grishin, Eugene V

2007-02-01

137

Evolutionary crossroads in developmental biology: Cnidaria  

PubMed Central

There is growing interest in the use of cnidarians (corals, sea anemones, jellyfish and hydroids) to investigate the evolution of key aspects of animal development, such as the formation of the third germ layer (mesoderm), the nervous system and the generation of bilaterality. The recent sequencing of the Nematostella and Hydra genomes, and the establishment of methods for manipulating gene expression, have inspired new research efforts using cnidarians. Here, we present the main features of cnidarian models and their advantages for research, and summarize key recent findings using these models that have informed our understanding of the evolution of the developmental processes underlying metazoan body plan formation. PMID:21389047

Technau, Ulrich; Steele, Robert E.

2011-01-01

138

Pyrosequencing reveals diverse microbial community associated with the zoanthid Palythoa australiae from the South China Sea.  

PubMed

Diverse sessile organisms inhabit the coral reef ecosystems, including corals, sponges, and sea anemones. In the past decades, scleractinian corals (Cnidaria, Anthozoa, Scleractinia) and their associated microorganisms have attracted much attention. Zoanthids (Cnidaria, Anthozoa, Zoanthidea) are commonly found in coral reefs. However, little is known about the community structure of zoanthid-associated microbiota. In this study, the microbial community associated with the zoanthid Palythoa australiae in the South China Sea was investigated by 454 pyrosequencing. As a result, 2,353 bacterial, 583 archaeal, and 36 eukaryotic microbial ribotypes were detected, respectively. A total of 22 bacterial phyla (16 formally described phyla and six candidate phyla) were recovered. Proteobacteria was the most abundant group, followed by Chloroflexi and Actinobacteria. High-abundance Rhizobiales and diverse Chloroflexi were observed in the bacterial community. The archaeal population was composed of Crenarchaeota and Euryarchaeota, with Marine Group I as the dominant lineage. In particular, Candidatus Nitrosopumilus dominated the archaeal community. Besides bacteria and archaea, the zoanthid harbored eukaryotic microorganisms including fungi and algae though their diversity was very low. This study provided the first insights into the microbial community associated with P. australiae by 454 pyrosequencing, consequently laid a basis for the understanding of the association of P. australiae-microbes symbioses. PMID:24682342

Sun, Wei; Zhang, Fengli; He, Liming; Li, Zhiyong

2014-05-01

139

SeaBase: a multispecies transcriptomic resource and platform for gene network inference.  

PubMed

Marine and aquatic animals are extraordinarily useful as models for identifying mechanisms of development and evolution, regeneration, resistance to cancer, longevity and symbiosis, among many other areas of research. This is due to the great diversity of these organisms and their wide-ranging capabilities. Genomics tools are essential for taking advantage of these "free lessons" of nature. However, genomics and transcriptomics are challenging in emerging model systems. Here, we present SeaBase, a tool for helping to meet these needs. Specifically, SeaBase provides a platform for sharing and searching transcriptome data. More importantly, SeaBase will support a growing number of tools for inferring gene network mechanisms. The first dataset available on SeaBase is a developmental transcriptomic profile of the sea anemone Nematostella vectensis (Anthozoa, Cnidaria). Additional datasets are currently being prepared and we are aiming to expand SeaBase to include user-supplied data for any number of marine and aquatic organisms, thereby supporting many potentially new models for gene network studies. SeaBase can be accessed online at: http://seabase.core.cli.mbl.edu. PMID:24907201

Fischer, Antje H L; Mozzherin, Dmitry; Eren, A Murat; Lans, Kristen D; Wilson, Nathan; Cosentino, Carlo; Smith, Joel

2014-07-01

140

Actiniaria and Scleractinia (Cnidaria, Anthozoa) from the Adriatic Sea (Croatia): First records, confirmed occurrences and significant range extensions of certain species  

Microsoft Academic Search

Among the Anthozoa collected in recent years from Croatian waters, two species of Actiniaria and eight species of Scleractinia (from 21 stations) deserve special mention. Here we document the first records in the Adriatic Sea of the actiniarians Alicia mirabilis and Halcampoides purpurea and the scleractinians Coenocyathus cylindricus, Spbenotrochus andrewianus and Bala?nophyllia regia, while the previously uncertain occurrence in this

Petar Kruzic; Helmut Zibrowius

2002-01-01

141

Interannual variability, growth, reproduction and feeding of Pelagia noctiluca (Cnidaria: Scyphozoa) in the Straits of Messina (Central Mediterranean Sea): Linkages with temperature and diet  

NASA Astrophysics Data System (ADS)

To identify some of the possible environmental factors stimulating the increasingly frequent outbreaks of the scyphomedusa Pelagia noctiluca in the Straits of Messina, we investigated its abundance, growth, reproduction and feeding over a 4-year period, from 2007 to 2011, at two coastal sites. Using either field investigations and manipulative experiments we show that, among the various factors considered, shifts in water temperature (influencing medusae metabolism, growth and reproduction rates) and the size structure of the zooplankton community (their natural preys) can promote the proliferation of P. noctiluca. In particular, we show that increased temperature let jellyfishes to grow more rapidly and reach exceptional sizes. We also report a peculiar opportunistic behavior of P. noctiluca, which makes this species a potentially strong competitor in the pelagic trophic web of the Straits ecosystem. We therefore propose that more frequent P. noctiluca outbreaks stimulated by increasing sea surface temperature and shifts in their prey availability and composition would become, in the near future, a major cause of ecosystem shift.

Rosa, S.; Pansera, M.; Granata, A.; Guglielmo, L.

2013-02-01

142

Anthozoa -Corals and Anemones  

E-print Network

· They will sting other corals and kill many of the hard and soft corals and polyps Corals and Coral Reefs · Types differs from hydrozoan polyps · Food enters mouth and then goes through tubular pharynx before entering ­ Longitudinal and circular muscles ­ Sea water in gastrovascular cavity acts as hydrostatic skeleton ­ Some

Cochran-Stafira, D. Liane

143

Phylogenetic relationships within the class Anthozoa (phylum Cnidaria) based on nuclear 18S rDNA sequences.  

PubMed

Taxonomic relationships within the corals and anemones (Phylum Cnidaria: Class Anthozoa) are based upon few morphological characters. The significance of any given character is debatable, and there is little fossil record available for deriving evolutionary relationships. We analyzed complete 18S ribosomal sequences to examine subclass-level and ordinal-level organization within the Anthozoa. We suggest that the Subclass Ceriantipatharia is not an evolutionarily relevant grouping. The Order Corallimorpharia appears paraphyletic and closely related to the Order Scleractinia. The 18S rRNA gene may be insufficient for establishing robust phylogenetic hypotheses concerning the specific relationships of the Corallimorpharia and the Ceriantharia and the branching sequence for the orders within the Hexacorallia. The 18S rRNA gene has sufficient phylogenetic signal, however, to distinguish among the major groupings within the Class Anthozoa, and we use this information to suggest relationships for the enigmatic taxa Dactylanthus and Dendrobrachia. PMID:10603268

Berntson, E A; France, S C; Mullineaux, L S

1999-11-01

144

Evolutionary Diversification of Banded Tube-Dwelling Anemones (Cnidaria; Ceriantharia; Isarachnanthus) in the Atlantic Ocean  

PubMed Central

The use of molecular data for species delimitation in Anthozoa is still a very delicate issue. This is probably due to the low genetic variation found among the molecular markers (primarily mitochondrial) commonly used for Anthozoa. Ceriantharia is an anthozoan group that has not been tested for genetic divergence at the species level. Recently, all three Atlantic species described for the genus Isarachnanthus of Atlantic Ocean, were deemed synonyms based on morphological simmilarities of only one species: Isarachnanthus maderensis. Here, we aimed to verify whether genetic relationships (using COI, 16S, ITS1 and ITS2 molecular markers) confirmed morphological affinities among members of Isarachnanthus from different regions across the Atlantic Ocean. Results from four DNA markers were completely congruent and revealed that two different species exist in the Atlantic Ocean. The low identification success and substantial overlap between intra and interspecific COI distances render the Anthozoa unsuitable for DNA barcoding, which is not true for Ceriantharia. In addition, genetic divergence within and between Ceriantharia species is more similar to that found in Medusozoa (Hydrozoa and Scyphozoa) than Anthozoa and Porifera that have divergence rates similar to typical metazoans. The two genetic species could also be separated based on micromorphological characteristics of their cnidomes. Using a specimen of Isarachnanthus bandanensis from Pacific Ocean as an outgroup, it was possible to estimate the minimum date of divergence between the clades. The cladogenesis event that formed the species of the Atlantic Ocean is estimated to have occured around 8.5 million years ago (Miocene) and several possible speciation scenarios are discussed. PMID:22815928

Stampar, Sergio N.; Maronna, Maximiliano M.; Vermeij, Mark J. A.; Silveira, Fabio L. d.; Morandini, André C.

2012-01-01

145

Characterization of Colletotrichum acutatum Causing Anthracnose of Anemone (Anemone coronaria L.)†  

PubMed Central

Anthracnose, or leaf-curl disease of anemone, caused by Colletotrichum sp., has been reported to occur in Australia, western Europe, and Japan. Symptoms include tissue necrosis, corm rot, leaf crinkles, and characteristic spiral twisting of floral peduncles. Three epidemics of the disease have been recorded in Israel: in 1978, in 1990 to 1993, and in 1996 to 1998. We characterized 92 Colletotrichum isolates associated with anthracnose of anemone (Anemone coronaria L.) for vegetative compatibility (72 isolates) and for molecular genotype (92 isolates) and virulence (4 isolates). Eighty-six of the isolates represented the three epidemics in Israel, one isolate was from Australia, and five isolates originated from western Europe. We divided these isolates into three vegetative-compatibility groups (VCGs). One VCG (ANE-A) included all 10 isolates from the first and second epidemics, and 13 of 62 examined isolates from the third epidemic in Israel, along with the isolate from Australia and 4 of 5 isolates from Europe. Another VCG (ANE-F) included most of the examined isolates (49 of the 62) from the third epidemic, as well as Colletotrichum acutatum from strawberry, in Israel. Based on PCR amplification with species-specific primers, all of the anemone isolates were identified as C. acutatum. Anemone and strawberry isolates of the two VCGs were genotypically similar and indistinguishable when compared by arbitrarily primed PCR of genomic DNA. Only isolate NL-12 from The Netherlands, confirmed as C. acutatum but not compatible with either VCG, had a distinct genotype; this isolate represents a third VCG of C. acutatum. Isolates from anemone and strawberry could infect both plant species in artificial inoculations. VCG ANE-F was recovered from natural infections of both anemone and strawberry, but VCG ANE-A was recovered only from anemone. This study of C. acutatum from anemone illustrates the potential of VCG analysis to reveal distinct subspecific groups within a pathogen population which appears to be genotypically homogeneous by molecular assays. PMID:11097901

Freeman, Stanley; Shabi, Ezra; Katan, Talma

2000-01-01

146

Macrofouling of deep-sea instrumentation after three years at 3690 m depth in the Charlie Gibbs fracture zone, mid-Atlantic ridge, with emphasis on hydroids (Cnidaria: Hydrozoa)  

NASA Astrophysics Data System (ADS)

Macrofouling is a common problem when deploying underwater instrumentation for long periods of time. It is a problem which can effect scientific experiments and monitoring missions though the creation of artificial reefs (thus increasing local biological activity) and reduce the quality of scientific data. Macrofouling is an issue typically considered to be restricted to the photic zones and is absent or negligible in the deep sea. To the contrary, the recovery of an accidentally lost deep-sea lander after 3 years submergence at 3960 m on the Mid-Atlantic Ridge (North Atlantic) revealed dense colonisation of macrofouling organisms. These organisms were found attached to all surfaces of the lander regardless of orientation and materials. The occurrence of such deep-sea macrofouling should be carefully investigated given the recent developments in long-term deep-sea observatory networks.

Blanco, R.; Shields, M. A.; Jamieson, A. J.

2013-12-01

147

Changes in zooxanthellae density, morphology, and mitotic index in hermatypic corals and anemones exposed to cyanide.  

PubMed

Sodium cyanide (NaCN) is widely used for the capture of reef fish throughout Southeast Asia and causes extensive fish mortality, but the effect of NaCN on reef corals remains debated. To document the impact of cyanide exposure on corals, the species Acropora millepora, Goniopora sp., Favites abdita, Trachyphyllia geoffrio, Plerogyra sp., Heliofungia actinformis, Euphyllia divisa, and Scarophyton sp., and the sea anemone Aiptasia pallida were exposed to varying concentrations of cyanide for varying time periods. Corals were exposed to 50, 100, 300, and 600 mg/l of cyanide ion (CN(-)) for 1-2 min (in seawater, the CN(-) forms hydrocyanic acid). These concentrations are much lower than those reportedly used by fish collectors. Exposed corals and anemones immediately retracted their tentacles and mesenterial filaments, and discharged copious amounts of mucus containing zooxanthellae. Gel electrophoreses techniques found changes in protein expression in both zooxanthellae and host tissue. Corals and anemones exposed to cyanide showed an immediate increase in mitotic cell division of their zooxenthellae, and a decrease in zooxanthellae density. In contrast, zooxanthellae cell division and density remained constant in controls. Histopathological changes included gastrodermal disruption, mesogleal degradation, and increased mucus in coral tissues. Zooxanthellae showed pigment loss, swelling, and deformation. Mortality occurred at all exposure levels. Exposed specimens experienced an increase in the ratio of gram-negative to gram-positive bacteria on the coral surface. The results demonstrate that exposure cyanide causes mortality to corals and anemones, even when applied at lower levels than that used by fish collectors. Even brief exposure to cyanide caused slow-acting and long-term damage to corals and their zooxanthellae. PMID:12735955

Cervino, J M; Hayes, R L; Honovich, M; Goreau, T J; Jones, S; Rubec, P J

2003-05-01

148

Distributed Anemone: Transparent Low-Latency Access to Remote Memory  

E-print Network

together the collective memory resources of multiple machines across a gi- gabit Ethernet LAN. Anemone treats remote memory as another level in the memory hierarchy between very fast local memory and very, and support for 'jumbo' Ethernet frames. Anemone achieves low page-fault latencies of 160µs average

Wang, Jian

149

AdE-1, a new inotropic Na(+) channel toxin from Aiptasia diaphana, is similar to, yet distinct from, known anemone Na(+) channel toxins.  

PubMed

Heart failure is one of the most prevalent causes of death in the western world. Sea anemone contains a myriad of short peptide neurotoxins affecting many pharmacological targets, several of which possess cardiotonic activity. In the present study we describe the isolation and characterization of AdE-1 (ion channel modifier), a novel cardiotonic peptide from the sea anemone Aiptasia diaphana, which differs from other cnidarian toxins. Although AdE-1 has the same cysteine residue arrangement as sea anemone type 1 and 2 Na(+) channel toxins, its sequence contains many substitutions in conserved and essential sites and its overall homology to other toxins identified to date is low (<36%). Physiologically, AdE-1 increases the amplitude of cardiomyocyte contraction and slows the late phase of the twitch relaxation velocity with no induction of spontaneous twitching. It increases action potential duration of cardiomyocytes with no effect on its threshold and on the cell's resting potential. Similar to other sea anemone Na(+) channel toxins such as Av2 (Anemonia viridis toxin II), AdE-1 markedly inhibits Na(+) current inactivation with no significant effect on current activation, suggesting a similar mechanism of action. However, its effects on twitch relaxation velocity, action potential amplitude and on the time to peak suggest that this novel toxin affects cardiomyocyte function via a more complex mechanism. Additionally, Av2's characteristic delayed and early after-depolarizations were not observed. Despite its structural differences, AdE-1 physiologic effectiveness is comparable with Av2 with a similar ED(50) value to blowfly larvae. This finding raises questions regarding the extent of the universality of structure-function in sea anemone Na(+) channel toxins. PMID:23356888

Nesher, Nir; Shapira, Eli; Sher, Daniel; Moran, Yehu; Tsveyer, Liora; Turchetti-Maia, Ana Luiza; Horowitz, Michal; Hochner, Binyamin; Zlotkin, Eliahu

2013-04-01

150

Microscaling: why larger anemones have longer cnidae.  

PubMed

Scaling analysis provides a quantitative method for describing and comparing how qualities of organisms vary as a function of body size. However, cell level phenomena have been notoriously hard to analyze because animal cells and organelles have such irregular shapes. The intracellular cnidae make good models of scaling at the cell level because they are durable and easy to image and measure. The mean length of unfired tentacle cnidae (spirocysts) varies continuously, and reversibly, with body size for three macrophagous anemone species. Significant differences in spirocyst shape and size relative to body mass are related to differences in tissue functions and species ecologies, strongly suggesting that cnida size, shape, and scaling patterns respond to natural selection. Cnida scaling patterns can be treated as features of cnidarian life histories. Spirocyst scaling exponents (slopes of log cnida dimension vs. log body weight) are similar to each other (0.05-0.09) and to reported values for animal somatic cells (0.017-0.17), but are much smaller than reported values for anemone basal diameters (0.30-0.38). I propose, here, a general, mechanical explanation for microscaling of structural secretory cells and their secretions, including the cnidae. Larger bodies require thicker, pliant sheets of sluggishly respiring extracellular support materials such as mesoglea and basement membrane. Thicker mesoglea can support larger, taller epithelial cells, which in turn provide additional maintenance services for these progressively thicker acellular layers. Ultimately, larger, taller cells can secrete and support larger, longer cnidae. PMID:15501853

Francis, Lisbeth

2004-10-01

151

The European athecate hydroids and their medusae (Hydrozoa, Cnidaria): Filifera Part 3  

E-print Network

belonging to Turritopsis dohrnii (Weismann, 1883). Keywords: Cnidaria - marine - Hydrozoa - Hydractiniidae of Turritopsis polycirrha (Keferstein, 1862). Hydractinia areolata Alder, 1862 is selected as type species

Schuchert, Peter

152

Mitochondrial genome of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa): A linear DNA molecule  

E-print Network

Mitochondrial genome of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa): A linear DNA molecule sequence of the linear mitochondrial DNA (mt-DNA) molecule of the moon jelly Aurelia aurita (Cnidaria are among the most atypical among Metazoa. First, among the four traditionally Gene 381 (2006) 92­101 www

Lavrov, Dennis V.

153

Lipids of gelatinous antarctic zooplankton: Cnidaria and Ctenophora  

Microsoft Academic Search

Antarctic gelatinous zooplankton, including Cnidaria (Calycopsis borchgrevinki, Diphyes antarctica, Stygiomedusa gigantea, Atolla wyvillei, Dimophyes arctica) and Ctenophora (Beroe cucumis, B. forskalii, Pleurobrachia pileus, Bolinopsis infundibulum) were collected near Elephant Island, South Shetland Islands, during January and February 1997 and 1998. Total lipid was\\u000a low in all zooplankton (0.1–5 mg g?1 wet mass) and included primarily polar lipids (59–96% of total

Matthew M. Nelson; Charles F. Phleger; Ben D. Mooney; Peter D. Nichols

2000-01-01

154

Searching for a Toxic Key to Unlock the Mystery of Anemonefish and Anemone Symbiosis  

PubMed Central

Twenty-six species of anemonefish of the genera Amphiprion and monospecific Premnas, use only 10 species of anemones as hosts in the wild (Families: Actiniidae, Stichodactylidae and Thalassianthidae). Of these 10 anemone species some are used by multiple species of anemonefish while others have only a single anemonefish symbiont. Past studies have explored the different patterns of usage between anemonefish species and anemone species; however the evolution of this relationship remains unknown and has been little studied over the past decade. Here we reopen the case, comparing the toxicity of crude venoms obtained from anemones that host anemonefish as a way to investigate why some anemone species are used as a host more than others. Specifically, for each anemone species we investigated acute toxicity using Artemia francisca (LC50), haemolytic toxicity using ovine erythrocytes (EC50) and neurotoxicity using shore crabs (Ozius truncatus). We found that haemolytic and neurotoxic activity varied among host anemone species. Generally anemone species that displayed greater haemolytic activity also displayed high neurotoxic activity and tend to be more toxic on average as indicated by acute lethality analysis. An overall venom toxicity ranking for each anemone species was compared with the number of anemonefish species that are known to associate with each anemone species in the wild. Interestingly, anemones with intermediate toxicity had the highest number of anemonefish associates, whereas anemones with either very low or very high toxicity had the fewest anemonefish associates. These data demonstrate that variation in toxicity among host anemone species may be important in the establishment and maintenance of anemonefish anemone symbiosis. PMID:24878777

Nedosyko, Anita M.; Young, Jeanne E.; Edwards, John W.; Burke da Silva, Karen

2014-01-01

155

Bioluminescent and Red-Fluorescent Lures in a Deep-Sea Siphonophore  

E-print Network

Bioluminescent and Red-Fluorescent Lures in a Deep-Sea Siphonophore Steven H. D. Haddock,1 * Casey W. Dunn,2 Philip R. Pugh,3 Christine E. Schnitzler1 Examples of bioluminescent lures in the sea (phylum Cnidaria) that are dominant predators in the ocean, some reaching tens of meters in length. Nearly

Dunn, Casey

156

Interannual variability in abundance of North Sea jellyfish and links to the North Atlantic Oscillation  

Microsoft Academic Search

Pronounced interannual variability in the abundance of medusae of the jellyfish species Aurelia aurita, Cyanea lamarckii, and Cyanea capillata (Phylum Cnidaria, Class Scyphozoa) in the North Sea was evident in data arising from the International Council for the Exploration of the Seas International 0-group Gadoid Surveys between 1971 and 1986. Possible climatic forcing of jellyfish abundance, via the North Atlantic

Christopher P. Lynam; Stephen J. Hay; Andrew S. Brierley

2004-01-01

157

Sea anemones (Actiniaria and Corallimorpharia) of Madang Province  

E-print Network

Eighteen species of Actiniaria and four of Corallimorpharia have been identified from waters less than 30 m deep in Madang Province. These include eight actinians host to nine species of anemonefishes.

Fautin, Daphne G.

1988-01-01

158

Visual ecology and functional morphology of cubozoa (cnidaria).  

PubMed

Jellyfish belong to one of the oldest extant animal phyla, the Cnidaria. The first Cnidaria appear in the fossil record 600 million years ago, preceeding the Cambrian explosion. They are an extremely successful group present in all marine environments and some freshwater environments. In contrast to many animal phyla in which vision is a primary sense Cnidarians do not, generally, employ image forming eyes. One small class stands alone: the Cubozoa. Cubomedusae are commonly known as box jellyfish. They possess image forming eyes (Coates et al., 2001) which certainly evolved independently from other metazoans. Cubomedusae therefore offer a unique perspective on the evolution of image forming eyes. This literature review collects, into one place, what is known about: the multiple eye types of box jellyfish, cubomedusan life history and ecology, and the sensory and neural systems of box jellyfish. Here I discuss how these features set cubomedusae apart from scyphomedusae and hydromedusae. Knowledge in these areas is sparse; the work done to date inspires increased efforts. PMID:21680462

Coates, Melissa M

2003-08-01

159

Transmission genetics of allorecognition in Hydractinia symbiolongicarpus (Cnidaria: Hydrozoa)  

SciTech Connect

Allorecognition is ubiquitous, or nearly so, amongst colonial invertebrates. Despite the prominent role that such phenomena have played both in evolutionary theory and in speculations on the origin of the vertebrate immune system, unambiguous data on the transmission genetics of fusibility (i.e., the ability of two individuals to fuse upon tissue contact) is lacking for any metazoan outside of the phylum Chordata. We have developed lines of the hydroid Hydractinia symbiolongicarpus (Phylum Cnidaria) inbred for fusibility and here report results of breeding experiments establishing that fusibility segregates as expected for a single locus with codominantly expressed alleles, with one shared allele producing a fusible phenotype. Surveys of fusibility in field populations and additional breeding experiments indicate the presence of an extensive allele series. 21 refs., 1 fig., 3 tabs.

Mokady, O.; Buss, L.W. [Yale Univ., New Haven, CT (United States)

1996-06-01

160

Circadian Clocks in the Cnidaria: Environmental Entrainment, Molecular Regulation, and Organismal Outputs  

PubMed Central

The circadian clock is a molecular network that translates predictable environmental signals, such as light levels, into organismal responses, including behavior and physiology. Regular oscillations of the molecular components of the clock enable individuals to anticipate regularly fluctuating environmental conditions. Cnidarians play important roles in benthic and pelagic marine environments and also occupy a key evolutionary position as the likely sister group to the bilaterians. Together, these attributes make members of this phylum attractive as models for testing hypotheses on roles for circadian clocks in regulating behavior, physiology, and reproduction as well as those regarding the deep evolutionary conservation of circadian regulatory pathways in animal evolution. Here, we review and synthesize the field of cnidarian circadian biology by discussing the diverse effects of daily light cycles on cnidarians, summarizing the molecular evidence for the conservation of a bilaterian-like circadian clock in anthozoan cnidarians, and presenting new empirical data supporting the presence of a conserved feed-forward loop in the starlet sea anemone, Nematostella vectensis. Furthermore, we discuss critical gaps in our current knowledge about the cnidarian clock, including the functions directly regulated by the clock and the precise molecular interactions that drive the oscillating gene-expression patterns. We conclude that the field of cnidarian circadian biology is moving rapidly toward linking molecular mechanisms with physiology and behavior. PMID:23620252

Reitzel, Adam M.; Tarrant, Ann M.; Levy, Oren

2013-01-01

161

New phylogenomic and comparative analyses provide corroborating evidence that Myxozoa is Cnidaria.  

PubMed

Myxozoa, a diverse group of morphologically simplified endoparasites, are well known fish parasites causing substantial economic losses in aquaculture. Despite active research, the phylogenetic position of Myxozoa remains ambiguous. After obtaining the genome and transcriptome data of the myxozoan Thelohanellus kitauei, we examined the phylogenetic position of Myxozoa from three different perspectives. First, phylogenomic analyses with the newly sequenced genomic data strongly supported the monophyly of Myxozoa and that Myxozoa is sister to Medusozoa within Cnidaria. Second, we detected two homologs to cnidarian-specific minicollagens in the T. kitauei genome with molecular characteristics similar to cnidarian-specific minicollagens, suggesting that the minicollagen homologs in T. kitauei may have functions similar to those in Cnidaria and that Myxozoa is Cnidaria. Additionally, phylogenetic analyses revealed that the minicollagens in myxozoans and medusozoans have a common ancestor. Third, we detected 11 of the 19 proto-mesodermalgenes in the T. kitauei genome, which were also present in the cnidarian Hydra magnipapillata, indicating Myxozoa is within Cnidaria. Thus, our results robustly support Myxozoa as a derived cnidarian taxon with an affinity to Medusozoa, helping to understand the diversity of the morphology, development and life cycle of Cnidaria and its evolution. PMID:25192780

Feng, Jin-Mei; Xiong, Jie; Zhang, Jin-Yong; Yang, Ya-Lin; Yao, Bin; Zhou, Zhi-Gang; Miao, Wei

2014-12-01

162

Benthic Community Composition and Seabed Characteristics of a Chukchi Sea Pockmark  

NASA Astrophysics Data System (ADS)

Several dozen seafloor features were mapped by Larry Mayer and his colleagues using swath bathymetry during a 2003 cruise with the USCGC HEALY near the eastern edge of the Chukchi Plateau (Chukchi Sea 76.6N, 163.9W). These were sub-circular depressions ranging from approximately 250 to over 1000m in width, with depths of up to 50m below the surrounding seabed, and situated in water depths from 500 to 950m. The origin of these features was undetermined, but one possibility was that they were pockmarks formed as a result of gas or fluid expulsion processes. We report here on benthic sampling undertaken at one of these pockmarks on 18 July 2005, also from USCGC HEALY. This elongated feature had maximum water depth of approximately 940m, was 1200m in maximum width, and was depressed approximately 40m below the surrounding seabed. The ocean in the vicinity of the pockmark was heavily ice-covered, which tightly restricted the ship's mobility during sampling operations. We used an ROV to collect and photograph the benthic epifauna during a 6h transit that crossed from the outside of the pockmark to near the center over a distance of 900m. We used a down-looking digital camera to collect over 800 pictures of the benthos at altitudes of 2 to 3m above the seabed. We also collected three cores with a 25x25cm box corer. Our investigations did not provide any direct evidence for gas or fluid flux through the seabed of this feature. Neither did we see any secondary indications of methane flux such as authigenic carbonates or bacterial mats. The abundance and diversity of benthic epifauna at this station was the highest among 8 stations sampled using similar methods during a 30 day cruise. The ROV observed brittle stars, various types of anemones, shrimps, eel pouts, stalked crinoids, benthic ctenophore (likely new species), burrows and mounts, gooseneck barnacles, mysids. Holothurians (c.f. Peneagone sp.) were the single most abundant group and were often photographed in densities of over 50 individuals per square meter. Preliminary analysis of the box core samples: Polychaetes (e.g. Chaetozone setose, Aricidea sp., Ophelina sp., Progoniada sp., Proclea graffi, Protula globifera), Foraminifera, Nemertini, Coronata (Cnidaria tubes), Sipunculida (Golfingia), Bivalvia, Anthozoa.

MacDonald, I. R.; Bluhm, B.; Iken, K.; Gagaev, S.; Robinson, S.

2005-12-01

163

Are Hox Genes Ancestrally Involved in Axial Patterning? Evidence from the Hydrozoan Clytia hemisphaerica (Cnidaria)  

PubMed Central

Background The early evolution and diversification of Hox-related genes in eumetazoans has been the subject of conflicting hypotheses concerning the evolutionary conservation of their role in axial patterning and the pre-bilaterian origin of the Hox and ParaHox clusters. The diversification of Hox/ParaHox genes clearly predates the origin of bilaterians. However, the existence of a “Hox code” predating the cnidarian-bilaterian ancestor and supporting the deep homology of axes is more controversial. This assumption was mainly based on the interpretation of Hox expression data from the sea anemone, but growing evidence from other cnidarian taxa puts into question this hypothesis. Methodology/Principal Findings Hox, ParaHox and Hox-related genes have been investigated here by phylogenetic analysis and in situ hybridisation in Clytia hemisphaerica, an hydrozoan species with medusa and polyp stages alternating in the life cycle. Our phylogenetic analyses do not support an origin of ParaHox and Hox genes by duplication of an ancestral ProtoHox cluster, and reveal a diversification of the cnidarian HOX9-14 genes into three groups called A, B, C. Among the 7 examined genes, only those belonging to the HOX9-14 and the CDX groups exhibit a restricted expression along the oral-aboral axis during development and in the planula larva, while the others are expressed in very specialised areas at the medusa stage. Conclusions/Significance Cross species comparison reveals a strong variability of gene expression along the oral-aboral axis and during the life cycle among cnidarian lineages. The most parsimonious interpretation is that the Hox code, collinearity and conservative role along the antero-posterior axis are bilaterian innovations. PMID:19156208

Chiori, Roxane; Jager, Muriel; Denker, Elsa; Wincker, Patrick; Da Silva, Corinne; Le Guyader, Hervé; Manuel, Michaël; Quéinnec, Eric

2009-01-01

164

CHARACTERIZATION OF MICROSATELLITE LOCI IN THE WIDELY INTRODUCED ESTUARINE ANEMONE NEMATOSTELLA VECTENSIS  

EPA Science Inventory

We characterized ten polymorphic microsatellite loci from Nematostella vectensis, a burrowing anemone recently introduced to estuaries along the Pacific coast of North America and the southeast coast of England. Preliminary results indicate high variability and significant depar...

165

Two new triterpenoid saponins from rhizome of Anemone amurensis.  

PubMed

Two new triterpenoid saponins were isolated from the 70% ethanol extract of the rhizome of Anemone amurensis, they are oleanolic acid 28-O-?-d-glucopyranosyl-(1 ? 3)-?-l-rhamnopyranosyl-(1 ? 4)-?-d-glucopyranosyl-(1 ? 6)-?-d-glucopyranosyl ester (1) and 23,27-dihydroxy oleanolic acid 3-O-?-l-arabinopyranoside (2). The structures of 1 and 2 were elucidated on the basis of chemical and spectral analysis, including 1D and 2D NMR data and HR-ESI-MS. Compounds 1 and 2 were tested for cytotoxicities against three human cancer cell lines (A549, Hep-G2, and MCF-7). Compound 1 showed potent cytotoxicity with IC50 values of 34.76, 41.17, and 28.92 ?M, respectively, while compound 2 with IC50>100 ?M. PMID:25486328

Lv, Chong-Ning; Fan, Li; Wang, Jing; Qin, Ru-Lan; Xu, Tan-Ye; Lei, Tian-Li; Lu, Jin-Cai

2014-12-01

166

Taxonomy and paleobiology of some Middle Cambrian Scenella (Cnidaria) and Hyolithids (Mollusca) from western North America  

E-print Network

THE UNIVERSITY OF KANSAS PALEONTOLOGICAL CONTRIBUTIONS December 29, 1988 Paper 121 TAXONOMY AND PALEOBIOLOGY OF SOME MIDDLE CAMBRIAN SCENELLA (CNIDARIA) AND HYOLITHIDS (MOLLUSCA) FROM WESTERN NORTH AMERICA' L. E. Babcock and R. A. Robison... MOLLUSCA Class HYOLITHA Order HYOLITHIDA Family HYOLITHIDAE Nicholson Remarks. —The emended family diagnosis of Malinky (1988:219-220) is followed here. The definition of Hyolithes was recently re- vised (Malinky, 1988; see also Malinky, Mapes...

Babcock, Loren E.; Robison, Richard A.

1988-12-29

167

UndertheSeaHouse JaimeMoskowitz,ColtonReavesandSavannaSalassi,ProgramDirectors  

E-print Network

relationship (such as clown fish and sea anemone). ACTION STEPS Members will create a PowerPoint presentation life as a hobby. By sharing our collective interests in fish, crustaceans, and marine mammals, we plan on sharing responsibility, such as having different jobs in regards to caring for our freshwater fish tank

Hayden, Nancy J.

168

Agent of Whirling Disease Meets Orphan Worm: Phylogenomic Analyses Firmly Place Myxozoa in Cnidaria  

PubMed Central

Myxozoa are microscopic obligate endoparasites with complex live cycles. Representatives are Myxobolus cerebralis, the causative agent of whirling disease in salmonids, and the enigmatic “orphan worm” Buddenbrockia plumatellae parasitizing in Bryozoa. Originally, Myxozoa were classified as protists, but later several metazoan characteristics were reported. However, their phylogenetic relationships remained doubtful. Some molecular phylogenetic analyses placed them as sister group to or even within Bilateria, whereas the possession of polar capsules that are similar to nematocysts of Cnidaria and of minicollagen genes suggest a close relationship between Myxozoa and Cnidaria. EST data of Buddenbrockia also indicated a cnidarian origin of Myxozoa, but were not sufficient to reject a closer relationship to bilaterians. Phylogenomic analyses of new genomic sequences of Myxobolus cerebralis firmly place Myxozoa as sister group to Medusozoa within Cnidaria. Based on the new dataset, the alternative hypothesis that Myxozoa form a clade with Bilateria can be rejected using topology tests. Sensitivity analyses indicate that this result is not affected by long branch attraction artifacts or compositional bias. PMID:23382916

Nesnidal, Maximilian P.; Helmkampf, Martin; Bruchhaus, Iris; El-Matbouli, Mansour; Hausdorf, Bernhard

2013-01-01

169

Influence of host anemone (Stichodactyla haddoni, saville-kent, 1893) locomotion on its resident anemonefish reproduction.  

PubMed

Anemonefish are found commonly in reef habitats and they select only limited anemone species to exist their life. This study describes the movement of anemone, Stichodactyla haddoni and its impact on the reproduction of anemonefishes in captivity. The anemone movement was found to be creeping and in straight-line between successive positions (based on overlay mapping). The distance traveled by the host with (60%) and without substrate (20%) varied significantly (P<0.05) at a random direction. Spawning interval of the resident fish (3-4 days) increased significantly to 8-12 days (P<0.001) because of anemone mobility. Furthermore, host locomotion affected the embryonic development of harbored fish clutches. Percentage of egg maturity (3.5-35.3%) showed an exponential decrease with increase in the distance of substrate from the clutches, from 98 to 100% in the absence of host mobility to 3.5-35% with movement of >60cm. This study concludes that the sedentary habitat would enhance the reproduction value of the harbored fish. Further, in captivity providing with a substrate, supplying regular feed and good light intensity could prevent anemone migration and its implications on the reproductive success of the anemonefish. PMID:23701833

Balamurugan, R J; Kumar, T T Ajith; Balasubramanian, T

2013-07-01

170

Similar specificities of symbiont uptake by adults and larvae in an anemone model system for coral biology.  

PubMed

Reef-building corals depend for much of their energy on photosynthesis by symbiotic dinoflagellate algae (genus Symbiodinium) that live within their gastrodermal cells. However, the cellular mechanisms underpinning this ecologically critical symbiosis, including those governing the specificity of symbiont uptake by the host, remain poorly understood, in part because of the difficulties of working with corals in the laboratory. Here, we used the small symbiotic sea anemone Aiptasia as an experimentally tractable model system to analyze the specificity and timing of symbiosis onset in larval and adult animals under controlled laboratory conditions. Using four clonal, axenic Symbiodinium strains, we found no difference in uptake specificity between larvae (even when very young) and adults. Although both compatible and incompatible algal strains were found within the larval guts, only the former appeared to be internalized by gastrodermal cells, and they (but not incompatible algae) proliferated rapidly within the larvae in the absence of detectable exchange with other larvae. Older larvae showed reduced ingestion of both compatible and incompatible algae, and the addition of food failed to promote the uptake of an incompatible algal strain. Thus, Aiptasia adults and larvae appear to have similar mechanisms for discriminating between compatible and incompatible dinoflagellate types prior to phagocytosis by host gastrodermal cells. Whether a particular algal strain is compatible or incompatible appears to be stable during years of axenic culture in the absence of a host. These studies provide a foundation for future analyses of the mechanisms of symbiont-uptake specificity in this emerging model system. PMID:24526722

Hambleton, Elizabeth A; Guse, Annika; Pringle, John R

2014-05-01

171

!Yes~igation of the Matina Svstem and Reproduction in The Pasque Flower (Anemone patens)  

Microsoft Academic Search

Klein Anne The buttercup family (Ranunculaceae) exhlbits a lot of variation in pollination methods and breeding strategies, where outcrossing (xenogamy) is typical, but self-fertilization (autogamy) has been observed. This study is an investigation of the breeding system and the pollination biology of Anemone patens, which exhibits both xenogamy and autogamy. Treatment groups were set up and analyzed to look at

Anne Mein

172

NUMERICAL SIMULATIONS OF CHROMOSPHERIC ANEMONE JETS ASSOCIATED WITH MOVING MAGNETIC FEATURES  

SciTech Connect

Observations with the space-based solar observatory Hinode show that small-scale magnetic structures in the photosphere are found to be associated with a particular class of jets of plasma in the chromosphere called anemone jets. The goal of our study is to conduct a numerical experiment of such chromospheric anemone jets related to the moving magnetic features (MMFs). We construct a 2.5 dimensional numerical MHD model to describe the process of magnetic reconnection between the MMFs and the pre-existing ambient magnetic field, which is driven by the horizontal motion of the magnetic structure in the photosphere. We include thermal conduction parallel to the magnetic field and optically thin radiative losses in the corona to account for a self-consistent description of the evaporation process during the heating of the plasma due to the reconnection process. The motion of the MMFs leads to the expected jet and our numerical results can reproduce many observed characteristics of chromospheric anemone jets, topologically and quantitatively. As a result of the tearing instability, plasmoids are generated in the reconnection process that are consistent with the observed bright moving blobs in the anemone jets. An increase in the thermal pressure at the base of the jet is also driven by the reconnection, which induces a train of slow-mode shocks propagating upward. These shocks are a secondary effect, and only modulate the outflow of the anemone jet. The jet itself is driven by the energy input due to the reconnection of the MMFs and the ambient magnetic field.

Yang, Liping; He, Jiansen; Tu, Chuanyi; Zhang, Lei [School of Earth and Space Sciences, Peking University, 100871 Beijing (China); Peter, Hardi [Max Planck Institute for Solar System Research, D-37191 Katlenburg-Lindau (Germany); Feng, Xueshang [SIGMA Weather Group, State Key Laboratory for Space Weather, Center for Space Science and Applied Research, Chinese Academy of Sciences, 100871 Beijing (China); Zhang, Shaohua, E-mail: jshept@gmail.com [Institute of Geology and Geophysics, Chinese Academy of Sciences, 100871 Beijing (China)

2013-11-01

173

P. Schuchert 2001a. Hydroids of Greenland and Iceland (Cnidaria, Hydrozoa). Meddelelser om Grnland 53: 1-184.  

E-print Network

P. Schuchert 2001a. Hydroids of Greenland and Iceland (Cnidaria, Hydrozoa). Meddelelser om Grønland 53: 1-184. Errata During the printing process, the Icelandic letter ð (eth) was occasionally changed be replaced by "C, BIOICE 2516..." page 172: "Material of Icelandic Museum of Natural History BIOICE material

Schuchert, Peter

174

A new species of Pseudomacrochiron Reddiah, 1969 (Crustacea: Copepoda: Macrochironidae) associated with scyphistomae of the moon jellyfish Aurelia sp. (Cnidaria: Scyphozoa) off Japan.  

PubMed

A new species of the Macrochironidae Humes & Boxshall, 1996 (Copepoda: Cyclopoida), Pseudomacrochiron aureliae n. sp., is described based on adult specimens extracted from the gastrovacular cavity of the scyphistomae of Aurelia sp. (Cnidaria: Scyphozoa) collected in the Seto Inland Sea and Ise Bay off the coast of Japan. The new species differs from its congeners by having the following combination of characters: a caudal ramus with a length to width ratio of 3.1; an accessory flagellum on caudal setae II, III and VI; three apical setae on the maxillule; only setae I and II on the maxillary basis; two short spines on the female maxilliped claw (endopod); an armature of III, I, 4 on the terminal exopodal segment of leg 3; an armature of I, II, 2 on the terminal endopodal segment of leg 3; an armature of II, I, 4 on the terminal exopodal segment of leg 4; and a short free exopodal segment of leg 5 (length to width ratio of 1.4) armed with a long seta and short spine. P. aureliae n. sp. is the first member of the genus reported from off Japan and from the scyphistomae of its scyphozoan host. PMID:22183922

Tang, Danny; Yasuda, Akira; Yamada, Satoshi; Nagasawa, Kazuya

2012-02-01

175

Developing the anemone Aiptasia as a tractable model for cnidarian-dinoflagellate symbiosis: the transcriptome of aposymbiotic A. pallida  

PubMed Central

Background Coral reefs are hotspots of oceanic biodiversity, forming the foundation of ecosystems that are important both ecologically and for their direct practical impacts on humans. Corals are declining globally due to a number of stressors, including rising sea-surface temperatures and pollution; such stresses can lead to a breakdown of the essential symbiotic relationship between the coral host and its endosymbiotic dinoflagellates, a process known as coral bleaching. Although the environmental stresses causing this breakdown are largely known, the cellular mechanisms of symbiosis establishment, maintenance, and breakdown are still largely obscure. Investigating the symbiosis using an experimentally tractable model organism, such as the small sea anemone Aiptasia, should improve our understanding of exactly how the environmental stressors affect coral survival and growth. Results We assembled the transcriptome of a clonal population of adult, aposymbiotic (dinoflagellate-free) Aiptasia pallida from ~208 million reads, yielding 58,018 contigs. We demonstrated that many of these contigs represent full-length or near-full-length transcripts that encode proteins similar to those from a diverse array of pathways in other organisms, including various metabolic enzymes, cytoskeletal proteins, and neuropeptide precursors. The contigs were annotated by sequence similarity, assigned GO terms, and scanned for conserved protein domains. We analyzed the frequency and types of single-nucleotide variants and estimated the size of the Aiptasia genome to be ~421?Mb. The contigs and annotations are available through NCBI (Transcription Shotgun Assembly database, accession numbers JV077153-JV134524) and at http://pringlelab.stanford.edu/projects.html. Conclusions The availability of an extensive transcriptome assembly for A. pallida will facilitate analyses of gene-expression changes, identification of proteins of interest, and other studies in this important emerging model system. PMID:22726260

2012-01-01

176

Molecular structure and chromosome distribution of three repetitive DNA families in Anemone hortensis L. (Ranunculaceae)  

Microsoft Academic Search

The structure, abundance and location of repetitive DNA sequences on chromosomes can characterize the nature of higher plant\\u000a genomes. Here we report on three new repeat DNA families isolated from Anemone hortensis L.; (i) AhTR1, a family of satellite DNA (stDNA) composed of a 554–561 bp long EcoRV monomer; (ii) AhTR2, a stDNA family composed of a 743 bp long HindIII monomer

Jelena Mlinarec; Mike Chester; Sonja Siljak-Yakovlev; Dražena Papeš; Andrew R. Leitch; Višnja Besendorfer

2009-01-01

177

Comparison of chromosome size in species of anemone and their hybrids  

Microsoft Academic Search

Total complement length has been determined in six species ofAnemone L. comprising 4 diploids (A. silvestris L.,A. virginiana L.,A. riparia Fern., andA. cylindrica Gray), 1 tetraploid (A. multifida Poir.), and 1 hexaploid (A. drummondii S. Wats.). Since chromosome complements of the parents fall in three definite size classes, the fate of the parental chromosomes can be followed in interclass hybrids.

Margaret Heimburger

1962-01-01

178

New records of Primnoidae (Cnidaria: Octocorallia) in Brazilian deep waters  

NASA Astrophysics Data System (ADS)

The knowledge of octocorals occurring in Brazilian deep waters is still lacking, with only a few studies conducted so far, most of which focused on large-scale marine habitats characterization. Primnoidae are common and characteristic of seamounts and deepwater coral banks, often providing habitat for other marine species. Although primnoids occur in all ocean basins, only Primnoella and Plumarella species were recorded along the Brazilian coast before this study. Primnoid specimens were obtained through dredging and remotely operated vehicles (ROV) sampling, collected by research projects conducted off the Brazilian coast, between 15 and 34°S. Taxonomic assessment resulted in 5 new records of Primnoidae genera in Brazil: Calyptrophora, Candidella, Dasystenella, Narella and Thouarella. The occurrences of Narella-off Salvador and Vitória, and in Campos Basin (935-1700 m), and Calyptrophora-in Campos Basin (1059-1152 m), are herein reported for the first time in the South Atlantic. Calyptrophora microdentata was previously known in Lesser Antilles, New England and Corner Rise Seamounts, between 686 and 2310 m. Candidella imbricata geographical distribution includes Western and Eastern Atlantic (514-2063 m and 815-2139 m, respectively), being registered herein in Campos Basin, between 1059 and 1605 m. Dasystenella acanthina collected off Rio Grande do Sul state (810 m) and occurs also off Argentina and Southern Ocean, between 150 and 5087 m. Plumarella diadema, which type locality is off São Sebastião, Brazil, has its geographical range extended northwards, occurring in Campos Basin (650 m). Thouarella koellikeri previously known for Patagonia and Antartic Peninsula, is registered for the off Brazil for the first time, in Campos Basin and off São Sebastião (609-659 m). There is a lot of work yet to be done in terms of taxonomic knowledge of Brazilian deep-sea octocorals. Research projects focusing on the investigations, including ROV sampling, of other geographical regions and depth ranges along Brazilian coast will certainly reveal other new octocorals occurrences and species.

Arantes, Renata C. M.; Loiola, Livia L.

2014-01-01

179

The Antitumor Effects of Triterpenoid Saponins from the Anemone flaccida and the Underlying Mechanism  

PubMed Central

Anemone flaccida Fr. Schmidt, a family of ancient hopanoids, have been used as traditional Asian herbs for the treatments of inflammation and convulsant diseases. Previous study on HeLa cells suggested that triterpenoid saponins from Anemone flaccida Fr. Schmidt may have potential antitumor effect due to their apoptotic activities. Here, we confirmed the apoptotic activities of the following five triterpenoid saponins: glycoside St-I4a (1), glycoside St-J (2), anhuienoside E (3), hedera saponin B (4), and flaccidoside II (5) on human BEL-7402 and HepG2 hepatoma cell lines, as well as the model of HeLa cells treated with lipopolysaccharide (LPS). We found that COX-2/PGE2 signaling pathway, which plays key roles in the development of cancer, is involved in the antitumor activities of these saponins. These data provide the evidence that triterpenoid saponins can induce apoptosis via COX-2/PGE2 pathway, implying a preventive role of saponins from Anemone flaccida in tumor. PMID:24191167

Han, Lin-Tao; Fang, Ying; Li, Ming-Ming; Yang, Hong-Bing; Huang, Fang

2013-01-01

180

The Antitumor Effects of Triterpenoid Saponins from the Anemone flaccida and the Underlying Mechanism.  

PubMed

Anemone flaccida Fr. Schmidt, a family of ancient hopanoids, have been used as traditional Asian herbs for the treatments of inflammation and convulsant diseases. Previous study on HeLa cells suggested that triterpenoid saponins from Anemone flaccida Fr. Schmidt may have potential antitumor effect due to their apoptotic activities. Here, we confirmed the apoptotic activities of the following five triterpenoid saponins: glycoside St-I4a (1), glycoside St-J (2), anhuienoside E (3), hedera saponin B (4), and flaccidoside II (5) on human BEL-7402 and HepG2 hepatoma cell lines, as well as the model of HeLa cells treated with lipopolysaccharide (LPS). We found that COX-2/PGE2 signaling pathway, which plays key roles in the development of cancer, is involved in the antitumor activities of these saponins. These data provide the evidence that triterpenoid saponins can induce apoptosis via COX-2/PGE2 pathway, implying a preventive role of saponins from Anemone flaccida in tumor. PMID:24191167

Han, Lin-Tao; Fang, Ying; Li, Ming-Ming; Yang, Hong-Bing; Huang, Fang

2013-01-01

181

Oregon's Rocky Shore Species: Anemones Giant Green Anemones get their bright coloration from symbiotic, single-celled  

E-print Network

Species: Molluscs Black Katy aka the leather chiton for it's leathery appearance Whelks, small littorine snails, and barnacles A common species, the finger limpet A small, colorful lined chiton A lemon nudibranch aka sea slug The underside of a gumboot chiton, the worlds largest chiton The mossy chiton gets

Wright, Dawn Jeannine

182

Functional Characterisation of a TRPM2 orthologue from the sea anemone Nematostella vectensis in human cells.  

PubMed

The human non-selective cation channel TRPM2 represents a mediator of apoptosis triggered by oxidative stress. The principal agonist ADP-ribose binds to the cytosolic domain of TRPM2, which is homologous to the human ADP-ribose pyrophosphatase NUDT9. To further elucidate the structure-function relationship of this channel, we characterised a TRPM2 orthologue from the cnidarian Nematostella vectensis, after its expression in a human cell line. This far distant relative shows only 31% total sequence similarity to hTRPM2, while its C-terminal domain has a greater resemblance to the NUDT9 enzyme. Current through nvTRPM2 was induced by ADPR, with a more pronounced sensitivity and faster kinetics than in hTRPM2. In contrast to hTRPM2, there was no response to H2O2 and hardly any modulatory effect by intracellular Ca(2+). The deletion of a stretch of 15 residues from the NUDT9 domain of nvTRPM2, which is absent in hTRPM2, did not change the response to ADPR but enabled activation of the channel by H2O2 and increased the effects of intracellular Ca(2+). These findings shed new light on the evolution of TRPM2 and establish nvTRPM2 as a promising tool to decipher its complex gating mechanisms. PMID:25620041

Kühn, Frank J P; Kühn, Cornelia; Lückhoff, Andreas

2015-01-01

183

The Association between the Hermit Crab Dardanus arrosor (Herbst) and the Sea Anemone Calliactis parasitica (Couch)  

Microsoft Academic Search

The association between Calliactis parasitica and the Mediterranean hermit crab, Dardanus arrosor, has been re-examined. It was confirmed that, unlike the Atlantic crab, Pagurus bernhardus, Dardanus arrosor can display an active behaviour pattern which assists the transfer of Calliactis to its shell. Extensive trials showed, however, a marked tendency for crabs to divide into two groups: 'performers', which show this

D. M. Ross; L. Sutton

1961-01-01

184

The Response of the Sea Anemone Calliactis parasitica to Shells of the Hermit Crab Pagurus bernhardus  

Microsoft Academic Search

The five stages in the behaviour pattern by which Calliactis parasitica transfers to shells of Buccinum, normally occupied by Pagurus bernhardus, are described. The first of these, the clinging of tentacles to shell, is a trigger for the pattern as a whole; it occurs in Calliactis already settled on glass or plastic, and more consistently, in Calliactis whose pedal disks

D. M. Ross; L. Sutton

1961-01-01

185

Protection of a hermit crab by its symbiotic sea anemone Calliactis tricolor  

Microsoft Academic Search

Zusammenfassung Experimenteller Nachweis, dass Einsiedlerkrebse,Pagurus pollicaris, mit symbiontischer Seeanemone,Calliactis tricolor, anf ihrer Schale vom natürlichen Feind der Krabbe,Calappa flammea, weitgehend gemieden werden.

R. B. McLean; R. N. Mariscal

1973-01-01

186

RGM Regulates BMP-Mediated Secondary Axis Formation in the Sea Anemone Nematostella vectensis.  

PubMed

Patterning of the metazoan dorsoventral axis is mediated by a complex interplay of BMP signaling regulators. Repulsive guidance molecule (RGM) is a conserved BMP coreceptor that has not been implicated in axis specification. We show that NvRGM is a key positive regulator of BMP signaling during secondary axis establishment in the cnidarian Nematostella vectensis. NvRGM regulates first the generation and later the shape of a BMP-dependent Smad1/5/8 gradient with peak activity on the side opposite the NvBMP/NvRGM/NvChordin expression domain. Full knockdown of Smad1/5/8 signaling blocks the formation of endodermal structures, the mesenteries, and the establishment of bilateral symmetry, while altering the gradient through partial NvRGM or NvBMP knockdown shifts the boundaries of asymmetric gene expression and the positioning of the mesenteries along the secondary axis. These findings provide insight into the diversification of axis specification mechanisms and identify a previously unrecognized role for RGM in BMP-mediated axial patterning. PMID:25482565

Leclère, Lucas; Rentzsch, Fabian

2014-12-11

187

Sexual reproduction of two intertidal sea anemones (Coelenteria: Actiniaria) in Malaysia  

E-print Network

Sexual reproduction in the actinians *Anthopleura handi* Dunn and *Haliplanella luciae* (Verrill) was studied through one year at Jeram, on the Malacca Straits. No evidence could be marshalled to support periodicity in gametogenesis of either...

Fautin, Daphne G.

1982-01-01

188

The effects of sexual and asexual reproduction on geographic variation in the sea anemone Actinia tenebrosa  

Microsoft Academic Search

Allelic and genotypic frequencies were determined for samples from 35 widely distributed Australasian colonies of Actinia tenebrosa and 2 South African colonies of A. equina. These data provided no evidence of gene flow between Australisian and South African Actinia colonies and indicated that there may be some restriction of gene flow between widely separated Australasian colonies.

D. J. Ayre

1984-01-01

189

Development and epithelial organisation of muscle cells in the sea anemone Nematostella vectensis  

PubMed Central

Introduction Nematostella vectensis, a member of the cnidarian class Anthozoa, has been established as a promising model system in developmental biology, but while information about the genetic regulation of embryonic development is rapidly increasing, little is known about the cellular organization of the various cell types in the adult. Here, we studied the anatomy and development of the muscular system of N. vectensis to obtain further insights into the evolution of muscle cells. Results The muscular system of N. vectensis is comprised of five distinct muscle groups, which are differentiated into a tentacle and a body column system. Both systems house longitudinal as well as circular portions. With the exception of the ectodermal tentacle longitudinal muscle, all muscle groups are of endodermal origin. The shape and epithelial organization of muscle cells vary considerably between different muscle groups. Ring muscle cells are formed as epitheliomuscular cells in which the myofilaments are housed in the basal part of the cell, while the apical part is connected to neighboring cells by apical cell-cell junctions. In the longitudinal muscles of the column, the muscular part at the basal side is connected to the apical part by a long and narrow cytoplasmic bridge. The organization of these cells, however, remains epitheliomuscular. A third type of muscle cell is represented in the longitudinal muscle of the tentacle. Using transgenic animals we show that the apical cell-cell junctions are lost during differentiation, resulting in a detachment of the muscle cells to a basiepithelial position. These muscle cells are still located within the epithelium and outside of the basal matrix, therefore constituting basiepithelial myocytes. We demonstrate that all muscle cells, including the longitudinal basiepithelial muscle cells of the tentacle, initially differentiate from regular epithelial cells before they alter their epithelial organisation. Conclusions A wide range of different muscle cell morphologies can already be found in a single animal. This suggests how a transition from an epithelially organized muscle system to a mesenchymal could have occurred. Our study on N. vectensis provides new insights into the organisation of a muscle system in a non-bilaterian organism. PMID:25009575

2014-01-01

190

Properties of a toxin from the sea anemone Stoichacis helianthus, including specific binding to sphingomyelin.  

PubMed Central

Stoichactis helianthus toxin, a protein derived presumably from the nematocysts, was purified to homogeneity. It has a molecular weight of about 16,000, an isoelectric pH of 9.8, and it contains approximately 3.7% carbohydrate. It is powerfully hemolytic for erythrocytes derived from a variety of animal species, those of the cat being the most sensitive and those of the guinea pig the most resistant. The toxin is lytic also for rabbit blood platelets, and it destroys cultured fibroblasts but is inactive for several kinds of bacterial protoplasts and spheroplasts. The hemolytic activity is specifically inhibited by sphingomyelin, and it is proposed that this phospholipid is the constituent of the membrane which functions as receptor for the toxin. Supporting evidence includes the findings that enzymes known to destroy sphingomyelin (a) prevent erythrocyte membranes from inhibiting hemolysis, and (b) render erythrocytes resistant to lysis by the toxin. The mechanism underlying hemolysis may involve translocation of membrane sphingomyelin by virtue of a specific affinity of the coelenterate protein for this phospholipid. PMID:1757

Bernheimer, A W; Avigad, L S

1976-01-01

191

From Sea Anemone to Homo sapiens: The Evolution of the p53 Family of Genes  

SciTech Connect

Levine, a leading authority on the molecular basis of cancer, gives a lecture on how the genes for p53 (genes that prevent cancer and preserve life) and related proteins have survived through a billion years of evolution.

Arnold Levine

2009-09-14

192

Unexpected complexity of the Wnt gene family in a sea anemone  

Microsoft Academic Search

The Wnt gene family encodes secreted signalling molecules that control cell fate in animal development and human diseases. Despite its significance, the evolution of this metazoan-specific protein family is unclear. In vertebrates, twelve Wnt subfamilies were defined, of which only six have counterparts in Ecdysozoa (for example, Drosophila and Caenorhabditis). Here, we report the isolation of twelve Wnt genes from

Arne Kusserow; Kevin Pang; Carsten Sturm; Martina Hrouda; Jan Lentfer; Heiko A. Schmidt; Ulrich Technau; Arndt von Haeseler; Bert Hobmayer; Mark Q. Martindale; Thomas W. Holstein

2005-01-01

193

Functional Characterisation of a TRPM2 orthologue from the sea anemone Nematostella vectensis in human cells  

PubMed Central

The human non-selective cation channel TRPM2 represents a mediator of apoptosis triggered by oxidative stress. The principal agonist ADP-ribose binds to the cytosolic domain of TRPM2, which is homologous to the human ADP-ribose pyrophosphatase NUDT9. To further elucidate the structure-function relationship of this channel, we characterised a TRPM2 orthologue from the cnidarian Nematostella vectensis, after its expression in a human cell line. This far distant relative shows only 31% total sequence similarity to hTRPM2, while its C-terminal domain has a greater resemblance to the NUDT9 enzyme. Current through nvTRPM2 was induced by ADPR, with a more pronounced sensitivity and faster kinetics than in hTRPM2. In contrast to hTRPM2, there was no response to H2O2 and hardly any modulatory effect by intracellular Ca2+. The deletion of a stretch of 15 residues from the NUDT9 domain of nvTRPM2, which is absent in hTRPM2, did not change the response to ADPR but enabled activation of the channel by H2O2 and increased the effects of intracellular Ca2+. These findings shed new light on the evolution of TRPM2 and establish nvTRPM2 as a promising tool to decipher its complex gating mechanisms. PMID:25620041

Kühn, Frank J. P.; Kühn, Cornelia; Lückhoff, Andreas

2015-01-01

194

Shallow-water zoantharians (Cnidaria, Hexacorallia) from the Central Indo-Pacific.  

PubMed

Despite the Central Indo-Pacific (CIP) and the Indonesian Archipelago being a well-known region of coral reef biodiversity, particularly in the 'Coral Triangle', little published information is available on its zoantharians (Cnidaria: Hexacorallia: Zoantharia). In order to provide a basis for future research on the Indo-Pacific zoantharian fauna and facilitate comparisons between more well-studied regions such as Japan and the Great Barrier Reef, this report deals with CIP zoantharian specimens in the Naturalis collection in Leiden, the Netherlands; 106 specimens were placed into 24 morpho-species and were supplemented with 88 in situ photographic records from Indonesia, the Philippines, and Papua New Guinea. At least nine morpho-species are likely to be undescribed species, indicating that the region needs more research in order to properly understand zoantharian diversity within the CIP. The Naturalis' zoantharian specimens are listed by species, as well as all relevant collection information, and in situ images are provided to aid in future studies on zoantharians in the CIP. PMID:25349499

Reimer, James D; Poliseno, Angelo; Hoeksema, Bert W

2014-01-01

195

Gross and microscopic morphology of lesions in Cnidaria from Palmyra Atoll, Central Pacific  

USGS Publications Warehouse

We conducted gross and microscopic characterizations of lesions in Cnidaria from Palmyra Atoll, Central Pacific. We found growth anomalies (GA) to be the most commonly encountered lesion. Cases of discoloration and tissue loss were rare. GAs had a focal or multi-focal distribution and were predominantly nodular, exophytic, and umbonate. In scleractinians, the majority of GAs manifested as hyperplasia of the basal body wall (52% of cases), with an associated absence or reduction of polyp structure (mesenteries and filaments, actinopharynx and tentacles), and depletion of zooxanthellae in the gastrodermis of the upper body wall. In the soft corals Sinularia sp. and Lobophytum sp., GAs exclusively manifested as prominent hyperplasia of the coenenchyme with an increased density of solenia. In contrast to scleractinians, soft coral GAs displayed an inflammatory and necrotizing component with marked edema of the mesoglea, accompanied by infiltrates of variably-sized granular amoebocytes. Fungi, algae, sponges, and Crustacea were present in some scleractinian GAs, but absent in soft coral GAs. Fragmentation of tissues was a common finding in Acropora acuminata and Montipora cf. dilatata colonies with tissue loss, although no obvious causative agents were seen. Discoloration in the zoanthid, Palythoa tuberculosa, was found to be the result of necrosis, while in Lobophytum sp. discoloration was the result of zooxanthellar depletion (bleaching). Soft corals with discoloration or tissue loss showed a marked inflammatory response, however no obvious causative organisms were seen. Lesions that appeared similar at the gross level were revealed to be distinct by microscopy, emphasizing the importance of histopathology.

Williams, Gareth J.; Work, Thierry M.; Aeby, Greta S.; Knapp, Ingrid S.; Davy, Simon K.

2011-01-01

196

Shallow-water zoantharians (Cnidaria, Hexacorallia) from the Central Indo-Pacific  

PubMed Central

Abstract Despite the Central Indo-Pacific (CIP) and the Indonesian Archipelago being a well-known region of coral reef biodiversity, particularly in the ‘Coral Triangle’, little published information is available on its zoantharians (Cnidaria: Hexacorallia: Zoantharia). In order to provide a basis for future research on the Indo-Pacific zoantharian fauna and facilitate comparisons between more well-studied regions such as Japan and the Great Barrier Reef, this report deals with CIP zoantharian specimens in the Naturalis collection in Leiden, the Netherlands; 106 specimens were placed into 24 morpho-species and were supplemented with 88 in situ photographic records from Indonesia, the Philippines, and Papua New Guinea. At least nine morpho-species are likely to be undescribed species, indicating that the region needs more research in order to properly understand zoantharian diversity within the CIP. The Naturalis’ zoantharian specimens are listed by species, as well as all relevant collection information, and in situ images are provided to aid in future studies on zoantharians in the CIP. PMID:25349499

Reimer, James D.; Poliseno, Angelo; Hoeksema, Bert W.

2014-01-01

197

Gross and microscopic morphology of lesions in Cnidaria from Palmyra Atoll, Central Pacific.  

PubMed

We conducted gross and microscopic characterizations of lesions in Cnidaria from Palmyra Atoll, Central Pacific. We found growth anomalies (GA) to be the most commonly encountered lesion. Cases of discoloration and tissue loss were rare. GAs had a focal or multi-focal distribution and were predominantly nodular, exophytic, and umbonate. In scleractinians, the majority of GAs manifested as hyperplasia of the basal body wall (52% of cases), with an associated absence or reduction of polyp structure (mesenteries and filaments, actinopharynx and tentacles), and depletion of zooxanthellae in the gastrodermis of the upper body wall. In the soft corals Sinularia sp. and Lobophytum sp., GAs exclusively manifested as prominent hyperplasia of the coenenchyme with an increased density of solenia. In contrast to scleractinians, soft coral GAs displayed an inflammatory and necrotizing component with marked edema of the mesoglea, accompanied by infiltrates of variably-sized granular amoebocytes. Fungi, algae, sponges, and Crustacea were present in some scleractinian GAs, but absent in soft coral GAs. Fragmentation of tissues was a common finding in Acropora acuminata and Montipora cf. dilatata colonies with tissue loss, although no obvious causative agents were seen. Discoloration in the zoanthid, Palythoa tuberculosa, was found to be the result of necrosis, while in Lobophytum sp. discoloration was the result of zooxanthellar depletion (bleaching). Soft corals with discoloration or tissue loss showed a marked inflammatory response, however no obvious causative organisms were seen. Lesions that appeared similar at the gross level were revealed to be distinct by microscopy, emphasizing the importance of histopathology. PMID:20709072

Williams, Gareth J; Work, Thierry M; Aeby, Greta S; Knapp, Ingrid S; Davy, Simon K

2011-02-01

198

A new species of antipatharian coral (Cnidaria: Anthozoa: Antipatharia: Schizopathidae) from the Pacific coast of Costa Rica  

SciTech Connect

A new species of black coral, Aphanipathes colombiana (Cnidaria:Antipatharia) from the Caribbean coast of Colombia is described. The species forms small flabellate colonies with anisomorphic polypar spines. It is morphologically similar to the western Atlantic species A. thyoides (Pourtales) but its hypostomal polypar spines are not reduced in size. The new species also resembles the Indo-Pacific species A. reticulata van Pesch but it has smooth-surfaced polypar spines, whereas in A. reticulata these spines have small tubercles on their surface

Opresko, Dennis M [ORNL; Breedy, Odalisca [University of Costa Rica

2010-09-01

199

SEA TURTLES Sea Turtles  

E-print Network

317 SEA TURTLES UNIT 24 Sea Turtles Unit 24 PROTECTED RESOURCES STAFF NMFS Office of Protected are listed as endangered. The authority to protect and conserve sea turtles in the marine environment for protection of sea turtles, their eggs, and hatchlings on land (nesting beaches). SPECIES AND STATUS Sea

200

Recent advances in deep-sea natural products.  

PubMed

Covering: 2009 to 2013. This review covers the 188 novel marine natural products described since 2008, from deep-water (50->5000 m) marine fauna including bryozoa, chordata, cnidaria, echinodermata, microorganisms, mollusca and porifera. The structures of the new compounds and details of the source organism, depth of collection and country of origin are presented, along with any relevant biological activities of the metabolites. Where reported, synthetic studies on the deep-sea natural products have also been included. Most strikingly, 75% of the compounds were reported to possess bioactivity, with almost half exhibiting low micromolar cytotoxicity towards a range of human cancer cell lines, along with a significant increase in the number of microbial deep-sea natural products reported. PMID:24871201

Skropeta, Danielle; Wei, Liangqian

2014-08-01

201

TREE vol. 14, no. 4 April 1999 0169-5347/99/$ see front matter 1999 Elsevier Science. All rights reserved. PII: S0169-5347(99)01588-8 127 Hydrozoa (Cnidaria) are common  

E-print Network

rights reserved. PII: S0169-5347(99)01588-8 127 Hydrozoa (Cnidaria) are common throughout the World Ocean- tiation of cells) and to bioluminescence with its associated green fluorescent pro- tein. The separate

Carrington, Emily

202

A conserved cluster of three PRD-class homeobox genes (homeobrain, rx and orthopedia) in the Cnidaria and Protostomia  

PubMed Central

Background Homeobox genes are a superclass of transcription factors with diverse developmental regulatory functions, which are found in plants, fungi and animals. In animals, several Antennapedia (ANTP)-class homeobox genes reside in extremely ancient gene clusters (for example, the Hox, ParaHox, and NKL clusters) and the evolution of these clusters has been implicated in the morphological diversification of animal bodyplans. By contrast, similarly ancient gene clusters have not been reported among the other classes of homeobox genes (that is, the LIM, POU, PRD and SIX classes). Results Using a combination of in silico queries and phylogenetic analyses, we found that a cluster of three PRD-class homeobox genes (Homeobrain (hbn), Rax (rx) and Orthopedia (otp)) is present in cnidarians, insects and mollusks (a partial cluster comprising hbn and rx is present in the placozoan Trichoplax adhaerens). We failed to identify this 'HRO' cluster in deuterostomes; in fact, the Homeobrain gene appears to be missing from the chordate genomes we examined, although it is present in hemichordates and echinoderms. To illuminate the ancestral organization and function of this ancient cluster, we mapped the constituent genes against the assembled genome of a model cnidarian, the sea anemone Nematostella vectensis, and characterized their spatiotemporal expression using in situ hybridization. In N. vectensis, these genes reside in a span of 33 kb with the same gene order as previously reported in insects. Comparisons of genomic sequences and expressed sequence tags revealed the presence of alternative transcripts of Nv-otp and two highly unusual protein-coding polymorphisms in the terminal helix of the Nv-rx homeodomain. A population genetic survey revealed the Rx polymorphisms to be widespread in natural populations. During larval development, all three genes are expressed in the ectoderm, in non-overlapping territories along the oral-aboral axis, with distinct temporal expression. Conclusion We report the first evidence for a PRD-class homeobox cluster that appears to have been conserved since the time of the cnidarian-bilaterian ancestor, and possibly even earlier, given the presence of a partial cluster in the placozoan Trichoplax. Very similar clusters comprising these three genes exist in Nematostella and diverse protostomes. Interestingly, in chordates, one member of the ancestral cluster (homeobrain) has apparently been lost, and there is no linkage between rx and orthopedia in any of the vertebrates. In Nematostella, the spatial expression of these three genes along the body column is not colinear with their physical order in the cluster but the temporal expression is, therefore, using the terminology that has been applied to the Hox cluster genes, the HRO cluster would appear to exhibit temporal but not spatial colinearity. It remains to be seen whether the mechanisms responsible for the evolutionary conservation of the HRO cluster are the same mechanisms responsible for cohesion of the Hox cluster and other ANTP-class homeobox clusters that have been widely conserved throughout animal evolution. PMID:20849646

2010-01-01

203

Biodiversity of the white coral bank off Cape Santa Maria di Leuca (Mediterranean Sea): An update  

NASA Astrophysics Data System (ADS)

The biodiversity of the Santa Maria di Leuca (SML) coral bank is summarized and its description is updated using data collected by means of underwater video systems, benthic samplers and fishing gears. A total of 222 living species have been recorded within the coral bank area in the depth range 280-1121 m. The most abundant benthic taxa recorded are Porifera (36 species) followed by Mollusca (35) and Cnidaria (31). The scleractinian corals Madrepora oculata and Lophelia pertusa are the main colonial species in the structure of the SML bank. Annelida, Crustacea and Bryozoa have been found with 24, 23 and 19 species, respectively. A total of 40 species of demersal fish have been recorded. Other faunal taxa were found with small numbers of species. One hundred and thirty-five species are new for the SML bank, 31 of which represent new records for the north-western Ionian Sea (2 Porifera, 17 Cnidaria, 1 Mollusca, 3 Annelida, 2 Crustacea, 4 Bryozoa and 4 Echinodermata). The finding of the annelid Harmothoë vesiculosa represents the first record for the Mediterranean Sea. The SML coral bank represents a biodiversity "hot-spot" on the bathyal bottoms of the Mediterranean Sea.

Mastrototaro, F.; D'Onghia, G.; Corriero, G.; Matarrese, A.; Maiorano, P.; Panetta, P.; Gherardi, M.; Longo, C.; Rosso, A.; Sciuto, F.; Sanfilippo, R.; Gravili, C.; Boero, F.; Taviani, M.; Tursi, A.

2010-03-01

204

The mitochondrial genome of Hydra oligactis (Cnidaria, Hydrozoa) sheds new light on animal mtDNA evolution and cnidarian phylogeny.  

PubMed

The 16,314-nuceotide sequence of the linear mitochondrial DNA (mtDNA) molecule of Hydra oligactis (Cnidaria, Hydrozoa)--the first from the class Hydrozoa--has been determined. This sequence contains genes for 13 energy pathway proteins, small and large subunit rRNAs, and methionine and tryptophan tRNAs, as is typical for cnidarians. All genes have the same transcriptional orientation and their arrangement in the genome is similar to that of the jellyfish Aurelia aurita. In addition, a partial copy of cox1 is present at one end of the molecule in a transcriptional orientation opposite to the rest of the genes, forming a part of inverted terminal repeat characteristic of linear mtDNA and linear mitochondrial plasmids. The sequence close to at least one end of the molecule contains several homonucleotide runs as well as small inverted repeats that are able to form strong secondary structures and may be involved in mtDNA maintenance and expression. Phylogenetic analysis of mitochondrial genes of H. oligactis and other cnidarians supports the Medusozoa hypothesis but also suggests that Anthozoa may be paraphyletic, with octocorallians more closely related to the Medusozoa than to the Hexacorallia. The latter inference implies that Anthozoa is paraphyletic and that the polyp (rather than a medusa) is the ancestral body type in Cnidaria. PMID:18222615

Kayal, Ehsan; Lavrov, Dennis V

2008-02-29

205

Growth of the Antarctic octocoral Primnoella scotiae and predation by the anemone Dactylanthus antarcticus  

NASA Astrophysics Data System (ADS)

Growth rates in Antarctic marine ectotherms have been demonstrated to be slowed by two to five times compared to shallow-water temperate species, with no previous reports for octocorals. Here growth rates were estimated in the single axis, non-branching Antarctic octocoral Primnoella scotiae using repeated in situ length measures covering both summer and winter periods, for tagged colonies from three sites at Signy Island over a two year period. Mean rates of length increase at the different sites ranged from 0.96 mm yr-1 to 55.3 mm yr-1. The fastest individual colony growth rate at any site ranged from 2.55 mm yr-1 to 175.6 mm yr-1. The mean of the fastest growth rates across all sites was 33.0 mm yr-1±14.7 (s.e.). Growth was significantly different between sites, and also between seasons and years. The mean overall increase in diameter of the average sized colony in the study (222.5 mm in axis length) was 0.053 mm yr-1. This is the slowest reported growth rate of any octocoral to date, and is five times slower than growth in most cold water octocorals. During the study it was noted that colonies were being attacked and consumed by the anemone Dactylanthus antarcticus. At one of the sites studied, between 5% and 8% of colonies surveyed were attacked each month. Anemone dispersal was via whole body inflation and drifting to new prey colonies that were attached to using tentacle-like column protuberances.

Peck, Lloyd S.; Brockington, Simon

2013-08-01

206

Leptohelia flexibilis gen. nov. et sp. nov., a remarkable deep-sea stylasterid (Cnidaria: Hydrozoa: Stylasteridae) from the southwest Pacific.  

PubMed

Leptohelia flexibilis gen. nov. et sp. nov., the first stylasterid with a combined calcified and non-calcified skeleton, is described from seamounts and the slope off the islands of New Caledonia, in the southwestern Pacific. The new species is distinguished from all other species of the family Stylasteridae by having a non-calcified organic axis, internal to the basal portion of the calcified corallum. The internal axis is flexible and enclosed by a series of up to 10 calcified annuli, allowing passive lateral bending of the colony. Molecular phylogenetic analyses confirm that Leptohelia flexibilis is a stylasterid coral and reveal that the species is closely related to Leptohelia microstylus comb. nov., a southwestern Pacific stylasterid that lacks an internal axis. PMID:25543758

Lindner, Alberto; Cairns, Stephen D; Zibrowius, Helmut

2014-01-01

207

Observations of Chromospheric Anemone Jets with Hinode CaII Broadband Filtergraph and Hida CaII Spectroheliograph  

NASA Astrophysics Data System (ADS)

We report on the first simultaneous observations of chromospheric ``anemone'' jets in solar active regions with the Hinode Solar Optical Telescope (SOT) CaII H broadband filtergraph and the CaII K spetroheliograph on the Domeless Solar Telescope (DST) at Hida Observatory. During the period of coordinated observations, nine chromospheric anemone jets were simultaneously observed with the two instruments. These observations revealed three important features: (1) the jets are generated in the lower chromosphere; i.e., these cannot be seen in CaII K3; (2) the length and lifetime of the jets are 0.4-5 Mm and 40-320 s, respectively; (3) the apparent velocity of the jets observed with the SOT is 3-24 km s-1, while the CaII K3 component at the jets shows a blueshift (in 5 events) in the range of 2-6 km s-1. The chromospheric anemone jets are associated with mixed polarity regions, which are either small emerging flux regions or moving magnetic features. It is found that the CaII K line often shows red or blue asymmetry in the K2/K1 component; the footpoint of the jets associated with emerging flux regions often shows a redshift (2-16 km s-1), while the one with moving magnetic features shows a blueshift (˜5 km s-1). A detailed analysis of the magnetic evolution of the jet-forming regions revealed that the reconnection rate (or canceling rate) of the total magnetic flux at the footpoint of the jets is on the order of 1016 Mx s-1, and the resulting magnetic energy release rate is (1.1-10) × 1024 erg s-1, with a total energy release of (1-13) × 1026 erg for the duration of the magnetic cancellation, ˜130 s. These are comparable to the estimated total energy, ˜1026 erg, in a single chromospheric anemone jet. In addition to the DST CaII K spectroheliogram and the SOT CaII H broadband filtergram, we also used for analysis an SOT magnetogram as well as a Hida H? filtergram. We present a physical model of the jet based on the observation, and discuss the relation between chromospheric anemone jets and Ellerman bombs.

Morita, Satoshi; Shibata, Kazunari; Ueno, Satoru; Ichimoto, Kiyoshi; Kitai, Reizaburo; Otsuji, Ken-Ichi

2010-08-01

208

Yolk formation in a stony coral Euphyllia ancora (Cnidaria, Anthozoa): insight into the evolution of vitellogenesis in nonbilaterian animals.  

PubMed

Vitellogenin (Vg) is a major yolk protein precursor in numerous oviparous animals. Numerous studies in bilateral oviparous animals have shown that Vg sequences are conserved across taxa and that Vgs are synthesized by somatic-cell lineages, transported to and accumulated in oocytes, and eventually used for supporting embryogenesis. In nonbilateral animals (Polifera, Cnidaria, and Ctenophora), which are regarded as evolutionarily primitive, although Vg cDNA has been identified in 2 coral species from Cnidaria, relatively little is known about the characteristics of yolk formation in their bodies. To address this issue, we identified and characterized 2 cDNA encoding yolk proteins, Vg and egg protein (Ep), in the stony coral Euphyllia ancora. RT-PCR analysis revealed that expression levels of both Vg and Ep increased in the female colonies as coral approached the spawning season. In addition, high levels of both Vg and Ep transcripts were detected in the putative ovarian tissue, as determined by tissue distribution analysis. Further analyses using mRNA in situ hybridization and immunohistochemistry determined that, within the putative ovarian tissue, these yolk proteins are synthesized in the mesenterial somatic cells but not in oocytes themselves. Furthermore, Vg proteins that accumulated in eggs were most likely consumed during the coral embryonic development, as assessed by immunoblotting. The characteristics of Vg that we identified in corals were somewhat similar to those of Vg in bilaterian oviparous animals, raising the hypothesis that such characteristics were likely present in the oogenesis of some common ancestor prior to divergence of the cnidarian and bilaterian lineages. PMID:23766130

Shikina, Shinya; Chen, Chieh-Jhen; Chung, Yi-Jou; Shao, Zi-Fan; Liou, Jhe-Yu; Tseng, Hua-Pin; Lee, Yan-Horn; Chang, Ching-Fong

2013-09-01

209

A Comprehensive Phylogenetic Analysis of the Scleractinia (Cnidaria, Anthozoa) Based on Mitochondrial CO1 Sequence Data  

PubMed Central

Background Classical morphological taxonomy places the approximately 1400 recognized species of Scleractinia (hard corals) into 27 families, but many aspects of coral evolution remain unclear despite the application of molecular phylogenetic methods. In part, this may be a consequence of such studies focusing on the reef-building (shallow water and zooxanthellate) Scleractinia, and largely ignoring the large number of deep-sea species. To better understand broad patterns of coral evolution, we generated molecular data for a broad and representative range of deep sea scleractinians collected off New Caledonia and Australia during the last decade, and conducted the most comprehensive molecular phylogenetic analysis to date of the order Scleractinia. Methodology Partial (595 bp) sequences of the mitochondrial cytochrome oxidase subunit 1 (CO1) gene were determined for 65 deep-sea (azooxanthellate) scleractinians and 11 shallow-water species. These new data were aligned with 158 published sequences, generating a 234 taxon dataset representing 25 of the 27 currently recognized scleractinian families. Principal Findings/Conclusions There was a striking discrepancy between the taxonomic validity of coral families consisting predominantly of deep-sea or shallow-water species. Most families composed predominantly of deep-sea azooxanthellate species were monophyletic in both maximum likelihood and Bayesian analyses but, by contrast (and consistent with previous studies), most families composed predominantly of shallow-water zooxanthellate taxa were polyphyletic, although Acroporidae, Poritidae, Pocilloporidae, and Fungiidae were exceptions to this general pattern. One factor contributing to this inconsistency may be the greater environmental stability of deep-sea environments, effectively removing taxonomic “noise” contributed by phenotypic plasticity. Our phylogenetic analyses imply that the most basal extant scleractinians are azooxanthellate solitary corals from deep-water, their divergence predating that of the robust and complex corals. Deep-sea corals are likely to be critical to understanding anthozoan evolution and the origins of the Scleractinia. PMID:20628613

Kitahara, Marcelo V.; Cairns, Stephen D.; Stolarski, Jaros?aw; Blair, David; Miller, David J.

2010-01-01

210

Imaging intracellular pH in a reef coral and symbiotic anemone.  

PubMed

The challenges corals and symbiotic cnidarians face from global environmental change brings new urgency to understanding fundamental elements of their physiology. Intracellular pH (pHi) influences almost all aspects of cellular physiology but has never been described in anthozoans or symbiotic cnidarians, despite its pivotal role in carbon concentration for photosynthesis and calcification. Using confocal microscopy and the pH sensitive probe carboxy SNARF-1, we mapped pHi in short-term light and dark-incubated cells of the reef coral Stylophora pistillata and the symbiotic anemone Anemonia viridis. In all cells isolated from both species, pHi was markedly lower than the surrounding seawater pH of 8.1. In cells that contained symbiotic algae, mean values of pHi were significantly higher in light treated cells than dark treated cells (7.41 +/- 0.22 versus 7.13 +/- 0.24 for S. pistillata; and 7.29 +/- 0.15 versus 7.01 +/- 0.27 for A. viridis). In contrast, there was no significant difference in pHi in light and dark treated cells without algal symbionts. Close inspection of the interface between host cytoplasm and algal symbionts revealed a distinct area of lower pH adjacent to the symbionts in both light and dark treated cells, possibly associated with the symbiosome membrane complex. These findings are significant developments for the elucidation of models of inorganic carbon transport for photosynthesis and calcification and also provide a cell imaging procedure for future investigations into how pHi and other fundamental intracellular parameters in corals respond to changes in the external environment such as reductions in seawater pH. PMID:19720994

Venn, A A; Tambutté, E; Lotto, S; Zoccola, D; Allemand, D; Tambutté, S

2009-09-29

211

Imaging intracellular pH in a reef coral and symbiotic anemone  

PubMed Central

The challenges corals and symbiotic cnidarians face from global environmental change brings new urgency to understanding fundamental elements of their physiology. Intracellular pH (pHi) influences almost all aspects of cellular physiology but has never been described in anthozoans or symbiotic cnidarians, despite its pivotal role in carbon concentration for photosynthesis and calcification. Using confocal microscopy and the pH sensitive probe carboxy SNARF-1, we mapped pHi in short-term light and dark-incubated cells of the reef coral Stylophora pistillata and the symbiotic anemone Anemonia viridis. In all cells isolated from both species, pHi was markedly lower than the surrounding seawater pH of 8.1. In cells that contained symbiotic algae, mean values of pHi were significantly higher in light treated cells than dark treated cells (7.41 ± 0.22 versus 7.13 ± 0.24 for S. pistillata; and 7.29 ± 0.15 versus 7.01 ± 0.27 for A. viridis). In contrast, there was no significant difference in pHi in light and dark treated cells without algal symbionts. Close inspection of the interface between host cytoplasm and algal symbionts revealed a distinct area of lower pH adjacent to the symbionts in both light and dark treated cells, possibly associated with the symbiosome membrane complex. These findings are significant developments for the elucidation of models of inorganic carbon transport for photosynthesis and calcification and also provide a cell imaging procedure for future investigations into how pHi and other fundamental intracellular parameters in corals respond to changes in the external environment such as reductions in seawater pH. PMID:19720994

Venn, A. A.; Tambutté, E.; Lotto, S.; Zoccola, D.; Allemand, D.; Tambutté, S.

2009-01-01

212

A genomewide survey of bHLH transcription factors in the coral Acropora digitifera identifies three novel orthologous families, pearl, amber, and peridot.  

PubMed

Decoding the genome of the coral, Acropora digitifera, enabled us to characterize a nearly full set of 70 basic helix-loop-helix (bHLH) transcription factors in this organism. This number is comparable to 68 bHLH genes in the sea anemone, Nematostella vectensis, and larger than those in most other invertebrate metazoans. The 70 bHLH genes were assigned to 29 orthologous families previously reported. In addition, we identified three novel HLH orthologous families, which we designated pearl, amber, and peridot, increasing the number of orthologous families to 32. Pearl and amber orthologues were found in genomes and expressed sequenced tags (ESTs) of Mollusca and Annelida in addition to Cnidaria. Peridot orthologues were found in genomes and ESTs of Cephalochordata and Hemichordata in addition to Cnidaria. These three genes were likely lost in the clades of Drosophila melanogaster, Caenorhabditis elegans, and Homo sapiens during animal evolution. PMID:22419240

Gyoja, Fuki; Kawashima, Takeshi; Satoh, Nori

2012-04-01

213

Micronesica 41(1): 117130, 2009 A sea anemone symbiotic with gastropods of eight species in the  

E-print Network

@ku.edu JOHN FUREY Department of Science and Mathematics, Northern Marianas College, Saipan, MP 96950 email: jfurey@saipan.com and MARYMEGAN DALY Department of Evolution, Ecology and Organismal Biology, Ohio State in shallow subtidal sand on Saipan and Tinian, Mariana Islands, in 1988 and from 2003 through 2007

Fautin, Daphne

214

Modeling habitat distribution from organism occurrences and environmental data: case study using anemonefishes and their sea anemone hosts  

E-print Network

. In this case study dealing with the continuum of qual- ity and/or extent inherent in habitat assessment, KGSMapper output ranks probability of matching habi- tat characteristics rather than a dichotomous occurrence or not of organisms; for this reason...: 269–283, 2006 base and front end (Oracle 9i RDBMS with Cold Fusion) with, on the server side, ArcIMS web-mapping software (www.esri.com/software/arcgis/arcims/index. html). Occurrence records are plotted in real time on a map through an XML-coded data...

Guinotte, J. M.; Bartley, J. D.; Iqbal, A.; Fautin, Daphne G.; Buddemeier, Robert W.

2006-07-03

215

Character evolution in light of phylogenetic analysis and taxonomic revision of the zooxanthellate sea anemone families Thalassianthidae and Aliciidae  

E-print Network

Aliciidae and Thalassianthidae look similar because they possess both morphological features of branched outgrowths and spherical defensive structures, and their identification can be confused because of their similarity. ...

Crowther, Andrea Louise

2013-05-31

216

vasa and nanos expression patterns in a sea anemone and the evolution of bilaterian germ cell specification mechanisms  

E-print Network

, but also in multiple somatic cell types during early embryogenesis. These results suggest one way in which Most bilaterians specify primordial germ cells (PGCs) during early embryogenesis using either inherited specified during embryogenesis (primordial germ cells or PGCs) is not significantly amplified, renewed

Extavour, Cassandra

217

Nanoporous TiO2 nanoparticle assemblies with mesoscale morphologies: nano-cabbage versus sea-anemone  

NASA Astrophysics Data System (ADS)

We report the novel synthesis of nanoporous TiO2 nanoparticle ensembles with unique mesoscale morphologies. Constituent nanoparticles evolved into multifaceted assemblies, exhibiting excellent crystallinity and enhanced photocatalytic activity compared with commercial TiO2. Such materials could be exploited for applications, like organic pollutant degradation.We report the novel synthesis of nanoporous TiO2 nanoparticle ensembles with unique mesoscale morphologies. Constituent nanoparticles evolved into multifaceted assemblies, exhibiting excellent crystallinity and enhanced photocatalytic activity compared with commercial TiO2. Such materials could be exploited for applications, like organic pollutant degradation. Electronic supplementary information (ESI) available: Synthesis and characterization procedures, TEM/XRD of samples prepared at different temperature and water content, table of nitrogen adsorption-desorption values of different samples. See DOI: 10.1039/c3nr06154j

Darbandi, Masih; Gebre, Tesfaye; Mitchell, Lucas; Erwin, William; Bardhan, Rizia; Levan, M. Douglas; Mochena, Mogus D.; Dickerson, James H.

2014-05-01

218

Introducing the starlet sea anemone Nematostella vectensis as a model for investigating microbial mediation of health and disease in hexacorals  

E-print Network

All animals in their natural state harbor complex communities of microbes including those that are beneficial (symbionts), neutral, or harmful (pathogens). The dynamic interactions between animals and their microbiota often ...

Har, Jia Yi

2009-01-01

219

Why is group size correlated with the size of the host sea anemone in the false clown anemonefish?  

Microsoft Academic Search

When social groups monopolize discrete habitat patches, group size may be positively correlated with patch size. The correlation can be a direct consequence of limited resources. Alternatively, it can be an indirect consequence of patch-size effects on a dominant group member. We asked which of these two mechanisms was responsible for a positive correlation between the group size of false

Jeremy S. Mitchell; Lawrence M. Dill

2005-01-01

220

Structure-function studies of tryptophan mutants of equinatoxin II, a sea anemone pore-forming protein.  

PubMed Central

Equinatoxin II (EqtII) is a eukaryotic cytolytic toxin that avidly creates pores in natural and model lipid membranes. It contains five tryptophan residues in three different regions of the molecule. In order to study its interaction with the lipid membranes, three tryptophan mutants, EqtII Trp(45), EqtII Trp(116/117) and EqtII Trp(149), were prepared in an Escherichia coli expression system [here, the tryptophan mutants are classified according to the position of the remaining tryptophan residue(s) in each mutated protein]. They all possess a single intrinsic fluorescent centre. All mutants were less haemolytically active than the wild-type, although the mechanism of erythrocyte damage was the same. EqtII Trp(116/117) resembles the wild-type in terms of its secondary structure content, as determined from Fourier-transform infrared (FTIR) spectra and its fluorescent properties. Tryptophans at these two positions are buried within the hydrophobic interior of the protein, and are transferred to the lipid phase during the interaction with the lipid membrane. The secondary structure of the other two mutants, EqtII Trp(45) and EqtII Trp(149), was altered to a certain extent. EqtII Trp(149) was the most dissimilar from the wild-type, displaying a higher content of random-coil structure. It also retained the lowest number of nitrogen-bound protons after exchange with (2)H(2)O, which might indicate a reduced compactness of the molecule. Tryptophans in EqtII Trp(45) and EqtII Trp(149) were more exposed to water, and also remained as such in the membrane-bound form. PMID:10657261

Malovrh, P; Barlic, A; Podlesek, Z; MaCek, P; Menestrina, G; Anderluh, G

2000-01-01

221

Anemone-like nanostructures for non-lithographic, reproducible, large-area, and ultra-sensitive SERS substrates.  

PubMed

The melt-infiltration technique enables the fabrication of complex nanostructures for a wide range of applications in optics, electronics, biomaterials, and catalysis. Here, anemone-like nanostructures are produced for the first time under the surface/interface principles of melt-infiltration as a non-lithographic method. Functionalized anodized aluminum oxide (AAO) membranes are used as templates to provide large-area production of nanostructures, and polycarbonate (PC) films are used as active phase materials. In order to understand formation dynamics of anemone-like structures finite element method (FEM) simulations are performed and it is found that wetting behaviour of the polymer is responsible for the formation of cavities at the caps of the structures. These nanostructures are examined in the surface-enhanced-Raman-spectroscopy (SERS) experiment and they exhibit great potential in this field. Reproducible SERS signals are detected with relative standard deviations (RSDs) of 7.2-12.6% for about 10,000 individual spots. SERS measurements are demonstrated at low concentrations of Rhodamine 6G (R6G), even at the picomolar level, with an enhancement factor of ?10(11). This high enhancement factor is ascribed to the significant electric field enhancement at the cavities of nanostructures and nanogaps between them, which is supported by finite difference time-domain (FDTD) simulations. These novel nanostructured films can be further optimized to be used in chemical and plasmonic sensors and as a single molecule SERS detection platform. PMID:25220106

Daglar, Bihter; Demirel, Gokcen Birlik; Khudiyev, Tural; Dogan, Tamer; Tobail, Osama; Altuntas, Sevde; Buyukserin, Fatih; Bayindir, Mehmet

2014-11-01

222

Anemone-like nanostructures for non-lithographic, reproducible, large-area, and ultra-sensitive SERS substrates  

NASA Astrophysics Data System (ADS)

The melt-infiltration technique enables the fabrication of complex nanostructures for a wide range of applications in optics, electronics, biomaterials, and catalysis. Here, anemone-like nanostructures are produced for the first time under the surface/interface principles of melt-infiltration as a non-lithographic method. Functionalized anodized aluminum oxide (AAO) membranes are used as templates to provide large-area production of nanostructures, and polycarbonate (PC) films are used as active phase materials. In order to understand formation dynamics of anemone-like structures finite element method (FEM) simulations are performed and it is found that wetting behaviour of the polymer is responsible for the formation of cavities at the caps of the structures. These nanostructures are examined in the surface-enhanced-Raman-spectroscopy (SERS) experiment and they exhibit great potential in this field. Reproducible SERS signals are detected with relative standard deviations (RSDs) of 7.2-12.6% for about 10 000 individual spots. SERS measurements are demonstrated at low concentrations of Rhodamine 6G (R6G), even at the picomolar level, with an enhancement factor of ~1011. This high enhancement factor is ascribed to the significant electric field enhancement at the cavities of nanostructures and nanogaps between them, which is supported by finite difference time-domain (FDTD) simulations. These novel nanostructured films can be further optimized to be used in chemical and plasmonic sensors and as a single molecule SERS detection platform.The melt-infiltration technique enables the fabrication of complex nanostructures for a wide range of applications in optics, electronics, biomaterials, and catalysis. Here, anemone-like nanostructures are produced for the first time under the surface/interface principles of melt-infiltration as a non-lithographic method. Functionalized anodized aluminum oxide (AAO) membranes are used as templates to provide large-area production of nanostructures, and polycarbonate (PC) films are used as active phase materials. In order to understand formation dynamics of anemone-like structures finite element method (FEM) simulations are performed and it is found that wetting behaviour of the polymer is responsible for the formation of cavities at the caps of the structures. These nanostructures are examined in the surface-enhanced-Raman-spectroscopy (SERS) experiment and they exhibit great potential in this field. Reproducible SERS signals are detected with relative standard deviations (RSDs) of 7.2-12.6% for about 10 000 individual spots. SERS measurements are demonstrated at low concentrations of Rhodamine 6G (R6G), even at the picomolar level, with an enhancement factor of ~1011. This high enhancement factor is ascribed to the significant electric field enhancement at the cavities of nanostructures and nanogaps between them, which is supported by finite difference time-domain (FDTD) simulations. These novel nanostructured films can be further optimized to be used in chemical and plasmonic sensors and as a single molecule SERS detection platform. Electronic supplementary information (ESI) available: SEM images of the AAO membrane and bare polymer film, FEM simulations of anemone-like polymeric nanopillars depending on the time and pressure, and detailed calculation of the enhancement factor both including experimental and theoretical approaches. See DOI: 10.1039/c4nr03909b

Daglar, Bihter; Demirel, Gokcen Birlik; Khudiyev, Tural; Dogan, Tamer; Tobail, Osama; Altuntas, Sevde; Buyukserin, Fatih; Bayindir, Mehmet

2014-10-01

223

Unravelling the nature of Waiparaconus, a pennatulacean (Cnidaria: Octocorallia) from the Late Mesozoic-Early Cainozoic of the Southern Hemisphere.  

PubMed

Enigmatic calcareous conical fossils have been known from marine Paleocene-Eocene sequences of New Zealand since the early 1870s. More recently, similar fossils have been recorded from both Late Cretaceous marine sequences of Western Australia, New Caledonia and Antarctica, and possibly from the Eocene of South America. The present paper extends the record to the late Cretaceous of New Caledonia. These remains are unlike any living taxa, and have been variously interpreted as molluscs (rudistid bivalves), cirripedes (stalked barnacles), annelids and inorganic structures. Assignation to the Cirripedia has been refuted by Buckeridge (1983, 1993), who proposed that the material would be better placed within the Cnidaria. We investigate this hypothesis in light of the New Caledonian material and by comparison with living gorgonians and pennatulaceans, and demonstrate that Waiparaconus is best placed within the Pennatulacea. Waiparaconus zelandicus varies in form somewhat, with 3 morphotypes defined and reinforced by geography. Comment is provided on the imperative to fit organic remains into known groups, with reflection on what may happen if taxa are left in insertae sedis. PMID:24673757

Buckeridge, John S; Campbell, Hamish J; Maurizot, Pierre

2014-03-01

224

Nematocyst discharge in Pelagia noctiluca (Cnidaria, Scyphozoa) oral arms can be affected by lidocaine, ethanol, ammonia and acetic acid.  

PubMed

Nematocyst discharge and concomitant delivery of toxins is triggered to perform both defence and predation strategies in Cnidarians, and may lead to serious local and systemic reactions in humans. Pelagia noctiluca (Cnidaria, Scyphozoa) is a jellyfish particularly abundant in the Strait of Messina (Italy). After accidental contact with this jellyfish, not discharged nematocysts or even fragments of tentacles or oral arms may tightly adhere to the human skin and, following discharge, severely increase pain and the other adverse consequences of the sting. The aim of the present study is to verify if the local anesthetic lidocaine and other compounds, like alcohols, acetic acid and ammonia, known to provide pain relief after jellyfish stings, may also affect in situ discharge of nematocysts. Discharge was induced by a combined physico-chemical stimulation of oral arms by chemosensitizers (such as N-acetylated sugars, aminoacids, proteins and nucleotides), in the presence or absence of 1% lidocaine, 70% ethanol, 5% acetic acid or 20% ammonia, followed by mechanical stimulation by a non-vibrating test probe. The above mentioned compounds failed to induce discharge per se, and dramatically impaired the chemosensitizer-induced discharge response. We therefore suggest that prompt local treatment of the stung epidermis with lidocaine, acetic acid, ethanol and ammonia may provide substantial pain relief and help in reducing possible harmful local and systemic adverse reaction following accidental contact with P. noctiluca specimens. PMID:24637105

Morabito, Rossana; Marino, Angela; Dossena, Silvia; La Spada, Giuseppa

2014-06-01

225

A Diploblastic Radiate Animal at the Dawn of Cambrian Diversification with a Simple Body Plan: Distinct from Cnidaria?  

PubMed Central

Background Microfossils of the genus Punctatus include developmental stages such as blastula, gastrula, and hatchlings, and represent the most complete developmental sequence of animals available from the earliest Cambrian. Despite the extremely well-preserved specimens, the evolutionary position of Punctatus has relied only on their conical remains and they have been tentatively assigned to cnidarians. We present a new interpretation of the Punctatus body plan based on the developmental reconstruction aided by recent advances in developmental biology. Results Punctatus developed from a rather large egg, gastrulated in a mode of invagination from a coeloblastura, and then formed a mouth directly from the blastopore. Spiny benthic hatchlings were distinguishable from swimming or crawling ciliate larvae found in cnidarians and sponges. A mouth appeared at the perihatching embryonic stage and was renewed periodically during growth, and old mouths transformed into the body wall, thus elongating the body. Growing animals retained a small blind gut in a large body cavity without partitioning by septa and did not form tentacles, pedal discs or holdfasts externally. A growth center at the oral pole was sufficient for body patterning throughout life, and the body patterning did not show any bias from radial symmetry. Conclusions Contrary to proposed cnidarian affinity, the Punctatus body plan has basic differences from that of cnidarians, especially concerning a spacious body cavity separating ectoderm from endoderm. The lack of many basic cnidarian characters in the body patterning of Punctatus leads us to consider its own taxonomic group, potentially outside of Cnidaria. PMID:23840375

Yasui, Kinya; Reimer, James D.; Liu, Yunhuan; Yao, Xiaoyong; Kubo, Daisuke; Shu, Degan; Li, Yong

2013-01-01

226

Sea Turtles  

NSDL National Science Digital Library

Sea World informational resource on all eight species of sea turtles. Excellent introduction to sea turtles including information on their classification, habitat, diet, reproduction, and much more. Includes photographs and illustrations throughout. Features two teaching activities for grades K-2.

227

Mitochondrial genome of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa): A linear DNA molecule encoding a putative DNA-dependent DNA polymerase.  

PubMed

The 16,937-nuceotide sequence of the linear mitochondrial DNA (mt-DNA) molecule of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa) - the first mtDNA sequence from the class Scypozoa and the first sequence of a linear mtDNA from Metazoa - has been determined. This sequence contains genes for 13 energy pathway proteins, small and large subunit rRNAs, and methionine and tryptophan tRNAs. In addition, two open reading frames of 324 and 969 base pairs in length have been found. The deduced amino-acid sequence of one of them, ORF969, displays extensive sequence similarity with the polymerase [but not the exonuclease] domain of family B DNA polymerases, and this ORF has been tentatively identified as dnab. This is the first report of dnab in animal mtDNA. The genes in A. aurita mtDNA are arranged in two clusters with opposite transcriptional polarities; transcription proceeding toward the ends of the molecule. The determined sequences at the ends of the molecule are nearly identical but inverted and lack any obvious potential secondary structures or telomere-like repeat elements. The acquisition of mitochondrial genomic data for the second class of Cnidaria allows us to reconstruct characteristic features of mitochondrial evolution in this animal phylum. PMID:16945488

Shao, Zhiyong; Graf, Shannon; Chaga, Oleg Y; Lavrov, Dennis V

2006-10-15

228

Identification of a new 6-bromo-5,9-eicosadienoic acid from the anemone Condylactis gigantea and the zoanthid Palythoa caribaeorum.  

PubMed

A new brominated fatty acid, 6-bromo-5,9-eicosadienoic acid [1] was identified in the phospholipids (mainly phosphatidylethanolamine) of the anemone Condylactis gigantea and the zoanthid Palythoa caribaeorum. The delta 5,9 fatty acids, 5,9-octadecadienoic acid, 5,9-eicosadienoic acid, 5,9-docosadienoic acid, and 5,9-tetracosadienoic acid, were also identified in both organisms. Structural elucidation was accomplished by spectroscopic and chemical means. Our results further corroborate that delta 5,9 phospholipid fatty acids are not unique to sponges, as recognized previously, but can be found in other marine invertebrates such as anemones and zoanthids. An improved procedure for the synthesis of picolinyl esters is also described. PMID:8594145

Carballeira, N M; Reyes, M

1995-11-01

229

RESEARCH ARTICLE Open Access Developing the anemone Aiptasia as a tractable  

E-print Network

Pringle* Abstract Background: Coral reefs are hotspots of oceanic biodiversity, forming the foundation, disease, destructive fishing practices, increased sea-surface temperatures, and ocean acidification all. Much recent research in coral biology has focused on the effects of stresses ­ particularly high

Pringle, John

230

Sea urchin  

NSDL National Science Digital Library

The sea urchin is a type of echinoderm. It is a consumer because it cannot make its own food and must eat other organisms to get energy. Sea urchins are omnivores, meaning they eat both plants and other animals to gain energy. Sea urchins have been known to eat algae, mussels, and sponges.

N/A N/A (NOAA;Channel Islands National Marine Sanctuary)

2004-12-23

231

Red Sea  

article title:  The Red Sea     View Larger Image ... Multi-angle Imaging SpectroRadiometer (MISR) image of the Red Sea was acquired on August 13, 2000. Located between the East African coast and the Saudi Arabian peninsula, the Red Sea got its name because the blooms of a type of algae,  Trichodesmium ...

2013-04-16

232

Cnidarian phylogenetic relationships as revealed by mitogenomics  

PubMed Central

Background Cnidaria (corals, sea anemones, hydroids, jellyfish) is a phylum of relatively simple aquatic animals characterized by the presence of the cnidocyst: a cell containing a giant capsular organelle with an eversible tubule (cnida). Species within Cnidaria have life cycles that involve one or both of the two distinct body forms, a typically benthic polyp, which may or may not be colonial, and a typically pelagic mostly solitary medusa. The currently accepted taxonomic scheme subdivides Cnidaria into two main assemblages: Anthozoa (Hexacorallia?+?Octocorallia) – cnidarians with a reproductive polyp and the absence of a medusa stage – and Medusozoa (Cubozoa, Hydrozoa, Scyphozoa, Staurozoa) – cnidarians that usually possess a reproductive medusa stage. Hypothesized relationships among these taxa greatly impact interpretations of cnidarian character evolution. Results We expanded the sampling of cnidarian mitochondrial genomes, particularly from Medusozoa, to reevaluate phylogenetic relationships within Cnidaria. Our phylogenetic analyses based on a mitochogenomic dataset support many prior hypotheses, including monophyly of Hexacorallia, Octocorallia, Medusozoa, Cubozoa, Staurozoa, Hydrozoa, Carybdeida, Chirodropida, and Hydroidolina, but reject the monophyly of Anthozoa, indicating that the Octocorallia?+?Medusozoa relationship is not the result of sampling bias, as proposed earlier. Further, our analyses contradict Scyphozoa [Discomedusae?+?Coronatae], Acraspeda [Cubozoa?+?Scyphozoa], as well as the hypothesis that Staurozoa is the sister group to all the other medusozoans. Conclusions Cnidarian mitochondrial genomic data contain phylogenetic signal informative for understanding the evolutionary history of this phylum. Mitogenome-based phylogenies, which reject the monophyly of Anthozoa, provide further evidence for the polyp-first hypothesis. By rejecting the traditional Acraspeda and Scyphozoa hypotheses, these analyses suggest that the shared morphological characters in these groups are plesiomorphies, originated in the branch leading to Medusozoa. The expansion of mitogenomic data along with improvements in phylogenetic inference methods and use of additional nuclear markers will further enhance our understanding of the phylogenetic relationships and character evolution within Cnidaria. PMID:23302374

2013-01-01

233

Comparative analysis of nuclear ribosomal DNA from the moon jelly Aurelia sp.1 (Cnidaria: Scyphozoa) with characterizations of the 18S, 28S genes, and the intergenic spacer (IGS)  

Microsoft Academic Search

Nuclear ribosomal DNAs (rDNA) constitute a multi-gene family with tandemly arranged units linked by an intergenic spacer (IGS).\\u000a Here we present the complete DNA sequence (7,731 bp) of a single repeat unit of an rDNA sequence from the moon jelly Aurelia sp.1 (Cnidaria: Scypozoa). The tandemly repeated rDNA units consisted of coding and non- coding regions, whose arrangement\\u000a was 18S

Jang-Seu Ki; Il-Chan Kim; Jae-Seong Lee

234

Sea Stars  

NSDL National Science Digital Library

At first glance, starfish, more properly called sea stars, aren’t doing much of anything. In this video, Jonathan’s investigations reveal a slow-motion predator that hunts and attacks its prey. Traveling the world, Jonathan investigates sea stars from the tropics to the Antarctic and uses time-lapse photography to reveal an amazing complexity to the world of the sea star. Please see the accompanying study guide for educational objectives and discussion points.

2012-07-28

235

Sea Turtles  

NSDL National Science Digital Library

In Malaysia there is an island known for more sea turtles than virtually anywhere on Earth. In this video, Jonathan visits this amazing ecosystem to learn about the life cycle of sea turtles. He is surprised to discover an amazingly complex and competitive environment. Please see the accompanying study guide for educational objectives and discussion points.

Productions, Jonathan B.

2010-03-29

236

The evolution of the four subunits of voltage-gated calcium channels: ancient roots, increasing complexity, and multiple losses.  

PubMed

The alpha subunits of voltage-gated calcium channels (Ca(v)s) are large transmembrane proteins responsible for crucial physiological processes in excitable cells. They are assisted by three auxiliary subunits that can modulate their electrical behavior. Little is known about the evolution and roles of the various subunits of Ca(v)s in nonbilaterian animals and in nonanimal lineages. For this reason, we mapped the phyletic distribution of the four channel subunits and reconstructed their phylogeny. Although alpha subunits have deep evolutionary roots as ancient as the split between plants and opistokonths, beta subunits appeared in the last common ancestor of animals and their close-relatives choanoflagellates, gamma subunits are a bilaterian novelty and alpha2/delta subunits appeared in the lineage of Placozoa, Cnidaria, and Bilateria. We note that gene losses were extremely common in the evolution of Ca(v)s, with noticeable losses in multiple clades of subfamilies and also of whole Ca(v) families. As in vertebrates, but not protostomes, Ca(v) channel genes duplicated in Cnidaria. We characterized by in situ hybridization the tissue distribution of alpha subunits in the sea anemone Nematostella vectensis, a nonbilaterian animal possessing all three Ca(v) subfamilies common to Bilateria. We find that some of the alpha subunit subtypes exhibit distinct spatiotemporal expression patterns. Further, all six sea anemone alpha subunit subtypes are conserved in stony corals, which separated from anemones 500 MA. This unexpected conservation together with the expression patterns strongly supports the notion that these subtypes carry unique functional roles. PMID:25146647

Moran, Yehu; Zakon, Harold H

2014-09-01

237

Caspian Sea  

NASA Technical Reports Server (NTRS)

In this Moderate-resolution Imaging Spectroradiometer (MODIS) image from December 3, 2001, winter sea ice can be seen forming in the shallow waters of the northern Caspian (left) and Aral (upper right) Seas. Despite the inflow of the Volga River (upper left), the northern portion of the Caspian Sea averages only 17 feet in depth, and responds to the region's continental climate, which is cold in winter and hot and dry in the summer. The southern part of the Sea is deeper and remains ice-free throughout the winter. The dirty appearance of the ice may be due to sediment in the water, but may also be due to wind-driven dust. The wind in the region can blow at hurricane-force strength and can cause the ice to pile up in hummocks that are anchored to the sea bottom. The eastern portion of the Aral Sea is also beginning to freeze. At least two characteristics of the Aral Sea 'compete' in determining whether its waters will freeze. The Sea is shallow, which increases the likelihood of freezing, but it is also very salty, which means that lower temperatures are required to freeze it than would be required for fresh water. With average December temperatures of 18oF, it's clearly cold enough to allow ice to form. As the waters that feed the Aral Sea continue to be diverted for agriculture, the Sea becomes shallower and the regional climate becomes even more continental. This is because large bodies of water absorb and retain heat, moderating seasonal changes in temperature. Image courtesy Jacques Descloitres, MODIS Land Rapid Response Team at NASA GSFC

2002-01-01

238

Biomass of Scyphozoan Jellyfish, and Its Spatial Association with 0-Group Fish in the Barents Sea  

PubMed Central

An 0-group fish survey is conducted annually in the Barents Sea in order to estimate fish population abundance. Data on jellyfish by-catch have been recorded since 1980, although this dataset has never been analysed. In recent years, however, the ecological importance of jellyfish medusae has become widely recognized. In this paper the biomass of jellyfish (medusae) in 0–60 m depths is calculated for the period 1980–2010. During this period the climate changed from cold to warm, and changes in zooplankton and fish distribution and abundance were observed. This paper discusses the less well known ecosystem component; jellyfish medusae within the Phylum Cnidaria, and their spatial and temporal variation. The long term average was ca. 9×108 kg, with some years showing biomasses in excess of 5×109 kg. The biomasses were low during 1980s, increased during 1990s, and were highest in early 2000s with a subsequent decline. The bulk of the jellyfish were observed in the central parts of the Barents Sea, which is a core area for most 0-group fishes. Jellyfish were associated with haddock in the western area, with haddock and herring in the central and coastal area, and with capelin in the northern area of the Barents Sea. The jellyfish were present in the temperature interval 1°CSea jellyfish medusae; however their biomass has showed a recent moderate decline during years with record high temperatures in the Barents Sea. Jellyfish are undoubtedly an important component of the Barents Sea ecosystem, and the data presented here represent the best summary of jellyfish biomass and distribution yet published for the region. PMID:22457732

Eriksen, Elena; Prozorkevich, Dmitry; Trofimov, Aleksandr; Howell, Daniel

2012-01-01

239

Sea Level  

NSDL National Science Digital Library

This problem provides an opportunity to increase familiarity with negative and positive numbers on a number line. The vertical number line is presented as black markings every one meter all the way up a lighthouse and on the underwater support going down to the sea bed, with sea level being "0". In answering the nine questions, children begin to calculate with negative numbers in the context of the distances between the sea creatures. The Teachers' Notes page offers suggestions for implementation, discussion questions, ideas for extension and support, and a link to a related resource, Swimming Pool (cataloged separately).

240

Two Streptomyces Species Producing Antibiotic, Antitumor, and Anti-Inflammatory Compounds Are Widespread Among Intertidal Macroalgae and Deep-Sea Coral Reef Invertebrates from the Central Cantabrian Sea.  

PubMed

Streptomycetes are widely distributed in the marine environment, although only a few studies on their associations to algae and coral ecosystems have been reported. Using a culture-dependent approach, we have isolated antibiotic-active Streptomyces species associated to diverse intertidal marine macroalgae (Phyllum Heterokontophyta, Rhodophyta, and Chlorophyta), from the central Cantabrian Sea. Two strains, with diverse antibiotic and cytotoxic activities, were found to inhabit these coastal environments, being widespread and persistent over a 3-year observation time frame. Based on 16S rRNA sequence analysis, the strains were identified as Streptomyces cyaneofuscatus M-27 and Streptomyces carnosus M-40. Similar isolates to these two strains were also associated to corals and other invertebrates from deep-sea coral reef ecosystem (Phyllum Cnidaria, Echinodermata, Arthropoda, Sipuncula, and Anelida) living up to 4.700-m depth in the submarine Avilés Canyon, thus revealing their barotolerant feature. These two strains were also found to colonize terrestrial lichens and have been repeatedly isolated from precipitations from tropospheric clouds. Compounds with antibiotic and cytotoxic activities produced by these strains were identified by high-performance liquid chromatography (HPLC) and database comparison. Antitumor compounds with antibacterial activities and members of the anthracycline family (daunomycin, cosmomycin B, galtamycin B), antifungals (maltophilins), anti-inflamatory molecules also with antituberculosis properties (lobophorins) were identified in this work. Many other compounds produced by the studied strains still remain unidentified, suggesting that Streptomyces associated to algae and coral ecosystems might represent an underexplored promising source for pharmaceutical drug discovery. PMID:25319239

Braña, Afredo F; Fiedler, Hans-Peter; Nava, Herminio; González, Verónica; Sarmiento-Vizcaíno, Aida; Molina, Axayacatl; Acuña, José L; García, Luis A; Blanco, Gloria

2014-10-16

241

Sea Legs  

NASA Astrophysics Data System (ADS)

Forty-foot, storm-swept seas, Spitzbergen polar bears roaming vast expanses of Arctic ice, furtive exchanges of forbidden manuscripts in Cold War Moscow, the New York city fashion scene, diving in mini-subs to the sea floor hot srings, life with the astronauts, romance and heartbreak, and invading the last bastions of male exclusivity: all are present in this fast-moving, non-fiction account of one woman' fascinating adventures in the world of marine geology and oceanography.

Macdonald, Kenneth C.

242

Student Experiments at Sea (SEAS)  

NSDL National Science Digital Library

Interdisciplinary curriculum introduces the scientific process, experimental design and data analysis. Units on plate tectonics, hydrothermal vents, ridge visualization, surveying deep sea organisms. Students become the scientists, developing proposals for at-sea investigations, teacher coordinates submission to researchers studying the East Pacific Rise. Students retrieve, organize, analyze and report data from their experiment. Past experimental journals are posted. Free registration required to access curriculum and submit proposal.

243

Parallel loss of nuclear-encoded mitochondrial aminoacyl-tRNA synthetases and mtDNA-encoded tRNAs in Cnidaria.  

PubMed

Unlike most animal mitochondrial (mt) genomes, which encode a set of 22 transfer RNAs (tRNAs) sufficient for mt protein synthesis, those of cnidarians have only retained one or two tRNA genes. Whether the missing cnidarian mt-tRNA genes relocated outside the main mt chromosome or were lost remains unclear. It is also unknown what impact the loss of tRNA genes had on other components of the mt translational machinery. Here, we explored the nuclear genome of the cnidarian Nematostella vectensis for the presence of mt-tRNA genes and their corresponding mt aminoacyl-tRNA synthetases (mt-aaRS). We detected no candidates for mt-tRNA genes and only two mt-aaRS orthologs. At the same time, we found that all but one cytosolic aaRS appear to be targeted to mitochondria. These results indicate that the loss of mt-tRNAs in Cnidaria is genuine and occurred in parallel with the loss of nuclear-encoded mt-aaRS. Our phylogenetic analyses of individual aaRS revealed that although the nearly total loss of mt-aaRS is rare, aaRS gene deletion and replacement have occurred throughout the evolution of Metazoa. PMID:20439315

Haen, Karri M; Pett, Walker; Lavrov, Dennis V

2010-10-01

244

Savage Seas  

NSDL National Science Digital Library

This companion site to the new PBS series offers a collection of informative pieces and activities centered around the world's oceans. The site is divided into four principal sections, each of which features an article, brief sidebars, video clips, and in some cases, animations. The first, The Captain's Bridge, explores shipwrecks, stormy seas, and ocean rescues. The second, The Crow's Nest, dives into the power of waves. The Deep Sea section takes users to the nether regions of the ocean, while The Weather Factory touches on cyclones, ice and icebergs, and El Nino. Additional features at the site include Ask the Expert, Facts from the Sea, an annotated collection of related sites, and information about the series.

245

Comparative analysis of nuclear ribosomal DNA from the moon jelly Aurelia sp.1 (Cnidaria: Scyphozoa) with characterizations of the 18S, 28S genes, and the intergenic spacer (IGS)  

Microsoft Academic Search

Nuclear ribosomal DNAs (rDNA) constitute a multi-gene family with tandemly arranged units linked by an intergenic spacer (IGS).\\u000a Here we present the complete DNA sequence (7,731 bp) of a single repeat unit of an rDNA sequence from the moon jelly Aurelia sp.1 (Cnidaria: Scypozoa). The tandemly repeated rDNA units consisted of coding and non-coding regions, whose arrangement\\u000a was 18S rDNA (1,814 bp,

Jang-Seu Ki; Il-Chan Kim; Jae-Seong Lee

2009-01-01

246

Sea Ice  

NASA Technical Reports Server (NTRS)

During 2013, Arctic sea ice extent remained well below normal, but the September 2013 minimum extent was substantially higher than the record-breaking minimum in 2012. Nonetheless, the minimum was still much lower than normal and the long-term trend Arctic September extent is -13.7 per decade relative to the 1981-2010 average. The less extreme conditions this year compared to 2012 were due to cooler temperatures and wind patterns that favored retention of ice through the summer. Sea ice thickness and volume remained near record-low levels, though indications are of slightly thicker ice compared to the record low of 2012.

Perovich, D.; Gerland, S.; Hendricks, S.; Meier, Walter N.; Nicolaus, M.; Richter-Menge, J.; Tschudi, M.

2013-01-01

247

Sea Launch  

NSDL National Science Digital Library

Sea Launch is an international satellite launch service company that has a unique way of delivering payloads into space. With the launch platform situated on the equator in the middle of the Pacific Ocean, a specially designed rocket propels satellites into orbit with very good accuracy. The Sea Launch home page has plenty of information about its operation, including an overview of the technology, statistics about its successes and failures, and Webcasts of many of its launches. A 200+ page user's guide goes into detail about all the various stages of a mission; everything from design considerations for the spacecraft to transportation to the launch site is mentioned in the document.

248

Sea ice in the China Sea  

SciTech Connect

In every winter, sea ice occurring in Bohai Sea and the North Yellow Sea is the first-year ice which is going through generating, developing and thawing processes. Therefore, it is necessary to spatially and temporally describe ice period, freezing range, thickness variations and general motion of sea ice. The purpose of this paper is to provide initial general situation and features of sea ice for forecasting and researching sea ice.

Deng Shuqi [National Research Center for Marine Environmental Forecasts, Beijing (China)

1993-12-31

249

Aral Sea  

... The retreating shoreline leaves the surface encrusted with salt and with agrochemicals brought in by the rivers. As the Sea's moderating ... Large Aral, and may be associated with windblown snow and/or salt particles carried aloft. The Multi-angle Imaging SpectroRadiometer ...

2013-04-16

250

Ross Sea  

article title:  Icebergs in the Ross Sea     View Larger Image Two large icebergs, designated B-15A and C-16, are captured in this Multi-angle Imaging ... the longitudinal quadrant in which it is first seen, and new icebergs sighted in that quadrant are sequentially numbered. B-15 divided from ...

2013-04-16

251

New taxa and revisionary systematics of alcyonacean octocorals from the Pacific coast of North America (Cnidaria, Anthozoa)  

PubMed Central

Abstract A taxonomic assessment of four species of octocorals from the northeastern Pacific Ocean (British Columbia to California) is provided. Included here are a new species of clavulariid stolonifieran Cryptophyton, a new species of the nephtheid soft coral Gersemia, an undetermined species of soft coral in the genus Alcyonium that has been referred in the literature by several other names, and a new genus is named for a plexaurid sea fan originally described in the Indo-Pacific genus Euplexaura. Discussions are included that compare the species to related taxa, or provide revisionary assessments. PMID:23794840

Williams, Gary C.

2013-01-01

252

Surficial geology of the sea floor in Long Island Sound offshore of Orient Point, New York  

USGS Publications Warehouse

The U.S. Geological Survey (USGS), the National Oceanic and Atmospheric Administration (NOAA), and the Connecticut Department of Environmental Protection (CT DEP) are working cooperatively to map and interpret features of the sea floor along the northeastern coast of the United States. This report presents multibeam bathymetry and sidescan-sonar data obtained during NOAA survey H11446, which was conducted in a 12-km2 area in Long Island Sound offshore of Orient Point, NY. In addition, sediment and photographic data from 26 stations obtained during a USGS verification cruise are presented. Overall, the sea floor slopes gently seaward, but topography is more complex in sand-wave and boulder areas, which are evident in the multibeam and sidescan-sonar data from the study area. Sand waves generally have north-south-oriented crests with 10- to 20-m wavelengths. Sand-wave asymmetry indicates eastward net sediment transport in the east and westward net sediment transport in the northern and western parts of the study area. Areas with boulders on the sea floor are typically hummocky and are part of a glacial moraine system. Boulders are typically encrusted with seaweed, sponges, and anemones as shown in the bottom photography.

McMullen, K.Y.; Poppe, L.J.; Danforth, W.W.; Blackwood, D.S.; Schaer, J.D.; Guberski, M.R.; Wood, D.A.; Doran, E.F.

2011-01-01

253

Revision of sea anemone genus *Epiactis* (Coelenterata: Actiniaria) on the Pacific coast of North America, with descriptions of two new brooding species  

E-print Network

*, and its type species, *C. ritteri*, is restored to the genus in which it was originally described. Thus, four species of *Epiactis* are known on the Pacific coast of North America: external brooders *E. prolifera* (type species of the genus) and *E...

Fautin, Daphne G.; Chia, Fu-Shiang

1986-01-01

254

Venom present in sea anemone (Heteractis magnifica) induces apoptosis in non-small-cell lung cancer A549 cells through activation of mitochondria-mediated pathway.  

PubMed

Lung cancer is a major cause of cancer deaths throughout the world and the complexity of apoptosis resistance in lung cancer is apparent. Venom from Heteractis magnifica caused dose-dependent decreases in survival of the human non-small-cell lung cancer cell line, as determined by the MTT and Crystal Violet assays. The H. magnifica venom induced cell cycle arrest and induced apoptosis of A549 cells, as confirmed by annexin V/propidium iodide staining. The venom-induced apoptosis in A549 cells was characterized by cleavage of caspase-3 and a reduction in the mitochondrial membrane potential. Interestingly, crude extracts from H. magnifica had less effect on the survival of non-cancer cell lines. In the non-cancer cells, the mechanism via which cell death occurred was through necrosis not apoptosis. These findings are important for future work using H. magnifica venom for pharmaceutical development to treat human lung cancer. PMID:24190482

Ramezanpour, Mahnaz; da Silva, Karen Burke; Sanderson, Barbara J S

2014-03-01

255

Hyperthermic stress-induced increase in the expression of glutamate-cysteine ligase and glutathione levels in the symbiotic sea anemone Aiptasia pallida  

PubMed Central

Hyperthermic stress is known to trigger the loss of unicellular algae from a number of symbiotic cnidarians, a phenomenon commonly referred to as bleaching. Oxidative and nitrosative stress have been suggested to play a major role during the process of bleaching, however the underlying molecular mechanisms are still poorly understood. In animals, the intracellular tripeptide glutathione (GSH) is involved in antioxidant defense, redox homeostasis and intracellular redox signaling. Therefore, we tested the hypothesis that hyperthermal stress-induced bleaching in Aiptasia pallida, a model for symbiotic cnidarians, results in increased levels of GSH synthesis. We report the cDNA sequence and functional analysis of the catalytic subunit of glutamate-cysteine ligase (GCLC), which catalyzes the rate-limiting step in GSH biosynthesis. In a time-series experiment, both GCLC gene expression and total GSH levels increased 4- and 1.5-fold, respectively, in response to hyperthermal stress. These results suggest that hyperthermal stress triggers adaptive increases in intracellular GSH biosynthesis in cnidarians as a protective response to oxidative/ nitrosative stress. Our results show the conserved function of GCLC and GSH across animals while placing a new perspective on the role of GSH in redox signaling during cnidarian bleaching. PMID:18602489

Sunagawa, Shinichi; Choi, Jinah; Forman, Henry Jay; Medina, Mónica

2009-01-01

256

From Sea to Shining Sea  

ERIC Educational Resources Information Center

Deep down in the depths of the sea, beautiful fish, mysterious ocean life, and unusual plants glimmer and glow in the eerie atmosphere of an ever-changing ocean. This article describes how, with this vision and a purpose in mind, three teachers pulled open classroom walls and joined forces so their second graders could create a mammoth 30 x 75"…

Scott, Beverly

2005-01-01

257

A Vertical Wall Dominated by Acesta excavata and Neopycnodonte zibrowii, Part of an Undersampled Group of Deep-Sea Habitats  

PubMed Central

We describe a novel biotope at 633 to 762 m depth on a vertical wall in the Whittard Canyon, an extensive canyon system reaching from the shelf to the deep sea on Ireland’s continental margin. We explored this wall with an ROV and compiled a photomosaic of the habitat. The assemblage contributing to the biotope was dominated by large limid bivalves, Acesta excavata (mean shell height 10.4 cm), and deep-sea oysters, Neopycnodonte zibrowii, at high densities, particularly at overhangs. Mean density of N. zibrowii increased with depth, with densities of the most closely packed areas of A. excavata also increasing with depth. Other taxa associated with the assemblage included the solitary coral Desmophyllum dianthus, cerianthid anemones, comatulid crinoids, the trochid gastropod Margarites sp., the portunid crab Bathynectes longispina and small fish of the family Bythitidae. The scleractinian coral Madrepora oculata, the pencil urchin Cidaris cidaris and a species of Epizoanthus were also common. Prominent but less abundant species included the flytrap anemone Actinoscyphia saginata, the carrier crab Paramola cuvieri, and the fishes Lepidion eques and Conger conger. Observations of the hydrography of the canyon system identified that the upper 500 m was dominated by Eastern North Atlantic Water, with Mediterranean Outflow Water beneath it. The permanent thermocline is found between 600 and 1000 m depth, i.e., in the depth range of the vertical wall and the dense assemblage of filter feeders. Beam attenuation indicated nepheloid layers present in the canyon system with the greatest amounts of suspended material at the ROV dive site between 500 and 750 m. A cross-canyon CTD transect indicated the presence of internal waves between these depths. We hypothesise that internal waves concentrate suspended sediment at high concentrations at the foot of the vertical wall, possibly explaining the large size and high density of filter-feeding molluscs. PMID:24260319

Johnson, Mark P.; White, Martin; Wilson, Annette; Würzberg, Laura; Schwabe, Enrico; Folch, Helka; Allcock, A. Louise

2013-01-01

258

A vertical wall dominated by Acesta excavata and Neopycnodonte zibrowii, part of an undersampled group of deep-sea habitats.  

PubMed

We describe a novel biotope at 633 to 762 m depth on a vertical wall in the Whittard Canyon, an extensive canyon system reaching from the shelf to the deep sea on Ireland's continental margin. We explored this wall with an ROV and compiled a photomosaic of the habitat. The assemblage contributing to the biotope was dominated by large limid bivalves, Acesta excavata (mean shell height 10.4 cm), and deep-sea oysters, Neopycnodonte zibrowii, at high densities, particularly at overhangs. Mean density of N. zibrowii increased with depth, with densities of the most closely packed areas of A. excavata also increasing with depth. Other taxa associated with the assemblage included the solitary coral Desmophyllum dianthus, cerianthid anemones, comatulid crinoids, the trochid gastropod Margarites sp., the portunid crab Bathynectes longispina and small fish of the family Bythitidae. The scleractinian coral Madrepora oculata, the pencil urchin Cidaris cidaris and a species of Epizoanthus were also common. Prominent but less abundant species included the flytrap anemone Actinoscyphia saginata, the carrier crab Paramola cuvieri, and the fishes Lepidion eques and Conger conger. Observations of the hydrography of the canyon system identified that the upper 500 m was dominated by Eastern North Atlantic Water, with Mediterranean Outflow Water beneath it. The permanent thermocline is found between 600 and 1000 m depth, i.e., in the depth range of the vertical wall and the dense assemblage of filter feeders. Beam attenuation indicated nepheloid layers present in the canyon system with the greatest amounts of suspended material at the ROV dive site between 500 and 750 m. A cross-canyon CTD transect indicated the presence of internal waves between these depths. We hypothesise that internal waves concentrate suspended sediment at high concentrations at the foot of the vertical wall, possibly explaining the large size and high density of filter-feeding molluscs. PMID:24260319

Johnson, Mark P; White, Martin; Wilson, Annette; Würzberg, Laura; Schwabe, Enrico; Folch, Helka; Allcock, A Louise

2013-01-01

259

Sea Ice  

NSDL National Science Digital Library

In this resource, students will discover that there are notable differences between sea ice and fresh-water ice, such as density. In on segment, students learn that the first sign of freezing on the sea is an oily appearance of the water caused by the formation of needle-like crystals. The site explains the relationship between growth and the rate at which heat flows from the water and that the ice pack can alter its shape and dimension due to the movement of winds, currents, thermal expansion, and contraction of the ice. Types of ice described here include new ice, nilas, young ice, first-year ice, and old ice while the forms of ice covered include pancake ice, brash ice, ice cake, floe, and fast ice. The site also explains the meteorological and oceanographic factors that control the amount and movement of ice.

260

Climate-mediated changes in zooplankton community structure for the eastern Bering Sea  

NASA Astrophysics Data System (ADS)

Zooplankton are critical to energy transfer between higher and lower trophic levels in the eastern Bering Sea ecosystem. Previous studies from the southeastern Bering Sea shelf documented substantial differences in zooplankton taxa in the Middle and Inner Shelf Domains between warm and cold years. Our investigation expands this analysis into the northern Bering Sea and the south Outer Domain, looking at zooplankton community structure during a period of climate-mediated, large-scale change. Elevated air temperatures in the early 2000s resulted in regional warming and low sea-ice extent in the southern shelf whereas the late 2000s were characterized by cold winters, extensive spring sea ice, and a well-developed pool of cold water over the entire Middle Domain. The abundance of large zooplankton taxa such as Calanus spp. (C. marshallae and C. glacialis), and Parasagitta elegans, increased from warm to cold periods, while the abundance of gelatinous zooplankton (Cnidaria) and small taxa decreased. Biomass followed the same trends as abundance, except that the biomass of small taxa in the southeastern Bering Sea remained constant due to changes in abundance of small copepod taxa (increases in Acartia spp. and Pseudocalanus spp. and decreases in Oithona spp.). Statistically significant changes in zooplankton community structure and individual species were greatest in the Middle Domain, but were evident in all shelf domains, and in both the northern and southern portions of the eastern shelf. Changes in community structure did not occur abruptly during the transition from warm to cold, but seemed to begin gradually and build as the influence of the sea ice and cold water temperatures persisted. The change occurred one year earlier in the northern than the southern Middle Shelf. These and previous observations demonstrate that lower trophic levels within the eastern Bering Sea respond to climate-mediated changes on a variety of time scales, including those shorter than the commonly accepted quasi-decadal time periods. This lack of resilience or inertia at the lowest trophic levels affects production at higher trophic levels and must be considered in management strategy evaluations of living marine resources.

Eisner, Lisa B.; Napp, Jeffrey M.; Mier, Kathryn L.; Pinchuk, Alexei I.; Andrews, Alexander G.

2014-11-01

261

A purified Palythoa venom fraction delays sodium current inactivation in sympathetic neurons.  

PubMed

Palythoa caribaeorum is a zoanthid (Phylum Cnidaria, class Anthozoa) commonly found in shallow waters of coral reefs along the Mexican Atlantic coast. Little is known on the pharmacological and biochemical properties of the venom components of this animal group. Toxin peptides from other cnidarian venoms, like sea anemones, target sodium and potassium voltage-gated channels. In this study, we tested the activity of a low molecular weight fraction from the venom of P. caribaeorum on voltage-gated sodium channels of the superior cervical ganglion (SCG) neurons of the rat. Our results showed that this fraction delays tetrodotoxin (TTX)-sensitive sodium channel inactivation indicated by a reversible 2-fold increase of the current at the decay. A peptide responsible for this activity was isolated and characterized. Its sequence showed that it does not resemble any previously reported toxin. Together, these results evidence the presence of neurotoxins in P. caribaeorum that act on sodium channels. PMID:24593961

Lazcano-Pérez, Fernando; Vivas, Oscar; Román-González, Sergio A; Rodríguez-Bustamante, Eduardo; Castro, Héctor; Arenas, Isabel; García, David E; Sánchez-Puig, Nuria; Arreguín-Espinosa, Roberto

2014-05-01

262

Mini-review: the evolution of neuropeptide signaling.  

PubMed

Neuropeptides and their G protein-coupled receptors (GPCRs) have an early evolutionary origin and are already abundant in basal animals with primitive nervous systems such as cnidarians (Hydra, jellyfishes, corals, and sea anemones). Most animals emerging after the Cnidaria belong to two evolutionary lineages, the Protostomia (to which the majority of invertebrates belong) and Deuterostomia (to which some minor groups of invertebrates, and all vertebrates belong). These two lineages split about 700 million years (Myr) ago. Many mammalian neuropeptide GPCRs have orthologues in the Protostomia and this is also true for some of the mammalian neuropeptides. Examples are oxytocin/vasopressin, GnRH, gastrin/CCK, and neuropeptide Y and their GPCRs. These results implicate that protostomes (for example insects and nematodes) can be used as models to study the biology of neuropeptide signaling. PMID:22726357

Grimmelikhuijzen, Cornelis J P; Hauser, Frank

2012-08-10

263

Mammals of the Sea.  

ERIC Educational Resources Information Center

Presents information on sea mammals, including definitions and characteristics of cetaceans, pinnipeds, and sirenians. Contains descriptions of the teaching activities "Whale Music,""Draw A Whale to Scale,""Adopt a Sea Mammal," and "Sea Mammal Sleuths." (TW)

Naturescope, 1986

1986-01-01

264

Tracking Sea Otters  

USGS Multimedia Gallery

USGS sea otter researcher Tim Tinker drives the boat on an expedition to track and observe sea otters in Monterey Bay, California. USGS scientists study sea otters in efforts to help the threatened species continue to recover from near extinction....

265

The non-Siphonophoran Hydrozoa (Cnidaria) of Salento, Italy with notes on their life-cycles: an illustrated guide.  

PubMed

The majority of Hydrozoa is represented by not readily noticeable, small species. In recent decades, however, taxonomic knowledge of the group has increased worldwide, with a significant number of investigations focused on the Mediterranean Sea. Over more than two decades, 115 species of hydrozoans were recorded from coastal waters along nearly 300 km of the Salento Peninsula (Apulia, Italy). For each species, records from different collections were merged into single sheets of a general database.        For each species, the following information is reported: description, cnidome, biology, occurrence in Salento, worldwide distribution, and bibliography. Descriptions refer to the benthic hydroid stage and, when present, also to the planktonic medusa stage. The 115 species of Hydrozoa, recorded along the Salento coastline, represent 25% of the Mediterranean Hydrozoa fauna (totaling 461 species), and nearly 3% of 3,702 world's known species covered in a recent monograph. Four species are non-indigenous, three of them with invasive behavior (Clytia hummelincki, Clytia linearis, and Eudendrium carneum), and one species now very common (Eudendrium merulum) in Salento. The complete life cycle of Clytia paulensis (Vanhöffen, 1910) is described for the first time.  PMID:25661429

Gravili, Cinzia; Vito, Doris De; Camillo, Cristina Gioia Di; Martell, Luis; Piraino, Stefano; Boero, Ferdinando

2015-01-01

266

Benthic meiofaunal composition and community structure in the Sethukuda mangrove area and adjacent open sea, East coast of India  

NASA Astrophysics Data System (ADS)

The ecological aspects of meiofaunal communities in the Muthupettai mangrove forest, East coast of India, has not been investigated in the last two decades. Surface water temperature ranged from 23.5 °C to 31.8 °C. Salinity varied from 24 to 34 ppt, while water pH fluctuated from 7.4 to 8.3. Dissolved oxygen concentration ranged from 3.86 to 5.33 mg/l. Meiofauna analysis in this study identified a total of 106 species from the mangrove and adjacent open sea area of Sethukuda. Among these, 56 species of foraminiferans, 20 species of nematodes, 7 species of harpacticoid copepods, 4 species of ostrocodes, and 2 species of rotifers were identified. Furthermore, a single species was identified from the following groups: ciliophora, cnidaria, gnathostomulida, insecta, propulida, bryozoa and polychaete larvae. Meiofaunal density varied between 12029 to 23493 individuals 10 cm/m2. The diversity index ranged from 3.515 to 3.680, species richness index varied from 6.384 to 8.497, and evenness index varied from 0.839 to 0876 in the mangrove area and adjacent open sea.

Thilagavathi, Balasubramanaian; Das, Bandana; Saravanakumar, Ayyappan; Raja, Kuzhanthaivel

2011-06-01

267

Sea Ice  

NASA Technical Reports Server (NTRS)

Sea ice covers vast areas of the polar oceans, with ice extent in the Northern Hemisphere ranging from approximately 7 x 10(exp 6) sq km in September to approximately 15 x 10(exp 6) sq km in March and ice extent in the Southern Hemisphere ranging from approximately 3 x 10(exp 6) sq km in February to approximately 18 x 10(exp 6) sq km in September. These ice covers have major impacts on the atmosphere, oceans, and ecosystems of the polar regions, and so as changes occur in them there are potential widespread consequences. Satellite data reveal considerable interannual variability in both polar sea ice covers, and many studies suggest possible connections between the ice and various oscillations within the climate system, such as the Arctic Oscillation, North Atlantic Oscillation, and Antarctic Oscillation, or Southern Annular Mode. Nonetheless, statistically significant long-term trends are also apparent, including overall trends of decreased ice coverage in the Arctic and increased ice coverage in the Antarctic from late 1978 through the end of 2003, with the Antarctic ice increases following marked decreases in the Antarctic ice during the 1970s. For a detailed picture of the seasonally varying ice cover at the start of the 21st century, this chapter includes ice concentration maps for each month of 2001 for both the Arctic and the Antarctic, as well as an overview of what the satellite record has revealed about the two polar ice covers from the 1970s through 2003.

Parkinson, Claire L.; Cavalieri, Donald J.

2005-01-01

268

Metazoan meiofauna in deep-sea canyons and adjacent open slopes: A large-scale comparison with focus on the rare taxa  

NASA Astrophysics Data System (ADS)

Metazoan meiofaunal abundance, total biomass, nematode size and the richness of taxa were investigated along bathymetric gradients (from the shelf break down to ca. 5000-m depth) in six submarine canyons and on five adjacent open slopes of three deep-sea regions. The investigated areas were distributed along >2500 km, on the Portuguese to the Catalan and South Adriatic margins. The Portuguese and Catalan margins displayed the highest abundances, biomass and richness of taxa, while the lowest values were observed in the Central Mediterranean Sea. The comparison between canyons and the nearby open slopes showed the lack of significant differences in terms of meiofaunal abundance and biomass at any sampling depth. In most canyons and on most slopes, meiofaunal variables did not display consistent bathymetric patterns. Conversely, we found that the different topographic features were apparently responsible for significant differences in the abundance and distribution of the rare meiofaunal taxa (i.e. taxa accounting for <1% of total meiofaunal abundance). Several taxa belonging to the temporary meiofauna, such as larvae/juveniles of Priapulida, Holothuroidea, Ascidiacea and Cnidaria, were encountered exclusively on open slopes, while others (including the Tanaidacea and Echinodea larvae) were found exclusively in canyons sediments. Results reported here indicate that, at large spatial scales, differences in deep-sea meiofaunal abundance and biomass are not only controlled by the available food sources, but also by the region or habitat specific topographic features, which apparently play a key role in the distribution of rare benthic taxa.

Bianchelli, S.; Gambi, C.; Zeppilli, D.; Danovaro, R.

2010-03-01

269

First evidence of inbreeding, relatedness and chaotic genetic patchiness in the holoplanktonic jellyfish Pelagia noctiluca (Scyphozoa, Cnidaria).  

PubMed

Genetic drift and non-random mating seldom influence species with large breeding populations and high dispersal potential, characterized by unstructured gene pool and panmixia at a scale lower than the minimum dispersal range of individuals. In the present study, a set of nine microsatellite markers was developed and used to investigate the spatio-temporal genetic patterns of the holoplanktonic jellyfish Pelagia noctiluca (Scyphozoa) in the Southern Tyrrhenian Sea. Homozygote excess was detected at eight loci, and individuals exhibited intra-population relatedness higher than expected by chance in at least three samples. This result was supported by the presence of siblings in at least 5 out 8 samples, 4 of which contained full-sib in addition to half-sib dyads. Having tested and ruled out alternative explanations as null alleles, our results suggest the influence of reproductive and behavioural features in shaping the genetic structure of P. noctiluca, as outcomes of population genetics analyses pointed out. Indeed, the genetic differentiation among populations was globally small but highlighted: a) a spatial genetic patchiness uncorrelated with distance between sampling locations, and b) a significant genetic heterogeneity between samples collected in the same locations in different years. Therefore, despite its extreme dispersal potential, P. noctiluca does not maintain a single homogenous population, but rather these jellyfish appear to have intra-bloom localized recruitment and/or individual cohesiveness, whereby siblings more likely swarm together as a single group and remain close after spawning events. These findings provide the first evidence of family structures and consequent genetic patchiness in a species with highly dispersive potential throughout its whole life cycle, contributing to understanding the patterns of dispersal and connectivity in marine environments. PMID:24977703

Aglieri, Giorgio; Papetti, Chiara; Zane, Lorenzo; Milisenda, Giacomo; Boero, Ferdinando; Piraino, Stefano

2014-01-01

270

First Evidence of Inbreeding, Relatedness and Chaotic Genetic Patchiness in the Holoplanktonic Jellyfish Pelagia noctiluca (Scyphozoa, Cnidaria)  

PubMed Central

Genetic drift and non-random mating seldom influence species with large breeding populations and high dispersal potential, characterized by unstructured gene pool and panmixia at a scale lower than the minimum dispersal range of individuals. In the present study, a set of nine microsatellite markers was developed and used to investigate the spatio-temporal genetic patterns of the holoplanktonic jellyfish Pelagia noctiluca (Scyphozoa) in the Southern Tyrrhenian Sea. Homozygote excess was detected at eight loci, and individuals exhibited intra-population relatedness higher than expected by chance in at least three samples. This result was supported by the presence of siblings in at least 5 out 8 samples, 4 of which contained full-sib in addition to half-sib dyads. Having tested and ruled out alternative explanations as null alleles, our results suggest the influence of reproductive and behavioural features in shaping the genetic structure of P. noctiluca, as outcomes of population genetics analyses pointed out. Indeed, the genetic differentiation among populations was globally small but highlighted: a) a spatial genetic patchiness uncorrelated with distance between sampling locations, and b) a significant genetic heterogeneity between samples collected in the same locations in different years. Therefore, despite its extreme dispersal potential, P. noctiluca does not maintain a single homogenous population, but rather these jellyfish appear to have intra-bloom localized recruitment and/or individual cohesiveness, whereby siblings more likely swarm together as a single group and remain close after spawning events. These findings provide the first evidence of family structures and consequent genetic patchiness in a species with highly dispersive potential throughout its whole life cycle, contributing to understanding the patterns of dispersal and connectivity in marine environments. PMID:24977703

Aglieri, Giorgio; Papetti, Chiara; Zane, Lorenzo; Milisenda, Giacomo; Boero, Ferdinando; Piraino, Stefano

2014-01-01

271

Impact of Climate Change on Hawaiian Monk Seals Activity 2: The Odd Couple  

E-print Network

Questions: · Why do some hermit crabs have sea anemones attached to their shells? · What are symbiotic hermit crabs and sea anemones, as well as Hawaiian monk seals and coral. Materials: · The "Odd Couple and photographed a hermit crab with what appeared to be several sea anemones attached to its shell. While none

272

Surficial geology of the sea floor in Central Rhode Island Sound Southeast of Point Judith, Rhode Island  

USGS Publications Warehouse

The U.S. Geological Survey (USGS) and the National Oceanic and Atmospheric Administration (NOAA) are working together to study sea-floor environments off the northeast coast of the United States. During 2008, NOAA survey H11996 collected multibeam echosounder data in a 65-square kilometer area in central Rhode Island Sound, southeast of Point Judith, Rhode Island. During 2010, the USGS collected bottom photographs and sediment samples from 25 stations in this study area. The bathymetry, photography, and sediment data are used to interpret sea-floor features including scour depressions, sand waves, trawl marks, and dredge spoils. Scour depressions cover the bathymetric highs in much of the study area. Sand waves are located mostly in the southwest, and trawl marks tend to be in the northern regions. Dredge spoils are located at a disposal site in a bathymetric low in the western end of the study area. Most stations have a sea-floor surface of sand or silty sand, but eight of the stations have boulders to pea-sized gravel or gravelly sediment on the surface. Photographs show sandy areas typically have scattered burrows, shells, amphipod communities, and worm tubes. Boulders and cobbles are commonly overgrown with hydrozoans and anemones.

McMullen, K.Y.; Poppe, L.J.; Ackerman, S.D.; Blackwood, D.S.; Schaer, J.D.; Nadeau, M.A.; Wood, D.A.

2011-01-01

273

Diversity, Distribution and Nature of Faunal Associations with Deep-Sea Pennatulacean Corals in the Northwest Atlantic  

PubMed Central

Anthoptilum grandiflorum and Halipteris finmarchica are two deep-sea corals (Octocorallia: Pennatulacea) common on soft bottoms in the North Atlantic where they are believed to act as biogenic habitat. The former also has a worldwide distribution. To assist conservation efforts, this study examines spatial and temporal patterns in the abundance, diversity, and nature of their faunal associates. A total of 14 species were found on A. grandiflorum and 6 species on H. finmarchica during a multi-year and multi-site sampling campaign in eastern Canada. Among those, 7 and 5 species, respectively, were attached to the sea pens and categorized as close associates or symbionts. Rarefaction analyses suggest that the most common associates of both sea pens have been sampled. Biodiversity associated with each sea pen is analyzed according to season, depth and region using either close associates or the broader collection of species. Associated biodiversity generally increases from northern to southern locations and does not vary with depth (?100–1400 m). Seasonal patterns in A. grandiflorum show higher biodiversity during spring/summer due to the transient presence of early life stages of fishes and shrimps whereas it peaks in fall for H. finmarchica. Two distinct endoparasitic species of highly modified copepods (families Lamippidae and Corallovexiidae) commonly occur in the polyps of A. grandiflorum and H. finmarchica, and a commensal sea anemone frequently associates with H. finmarchica. Stable isotope analyses (?13C and ?15N) reveal potential trophic interactions between the parasites and their hosts. Overall, the diversity of obligate/permanent associates of sea pens is moderate; however the presence of mobile/transient associates highlights an ecological role that has yet to be fully elucidated and supports their key contribution to the enhancement of biodiversity in the Northwest Atlantic. PMID:25369515

Baillon, Sandrine; Hamel, Jean-François; Mercier, Annie

2014-01-01

274

The Discovery of New Deep-Sea Hydrothermal Vent Communities in the Southern Ocean and Implications for Biogeography  

PubMed Central

Since the first discovery of deep-sea hydrothermal vents along the Galápagos Rift in 1977, numerous vent sites and endemic faunal assemblages have been found along mid-ocean ridges and back-arc basins at low to mid latitudes. These discoveries have suggested the existence of separate biogeographic provinces in the Atlantic and the North West Pacific, the existence of a province including the South West Pacific and Indian Ocean, and a separation of the North East Pacific, North East Pacific Rise, and South East Pacific Rise. The Southern Ocean is known to be a region of high deep-sea species diversity and centre of origin for the global deep-sea fauna. It has also been proposed as a gateway connecting hydrothermal vents in different oceans but is little explored because of extreme conditions. Since 2009 we have explored two segments of the East Scotia Ridge (ESR) in the Southern Ocean using a remotely operated vehicle. In each segment we located deep-sea hydrothermal vents hosting high-temperature black smokers up to 382.8°C and diffuse venting. The chemosynthetic ecosystems hosted by these vents are dominated by a new yeti crab (Kiwa n. sp.), stalked barnacles, limpets, peltospiroid gastropods, anemones, and a predatory sea star. Taxa abundant in vent ecosystems in other oceans, including polychaete worms (Siboglinidae), bathymodiolid mussels, and alvinocaridid shrimps, are absent from the ESR vents. These groups, except the Siboglinidae, possess planktotrophic larvae, rare in Antarctic marine invertebrates, suggesting that the environmental conditions of the Southern Ocean may act as a dispersal filter for vent taxa. Evidence from the distinctive fauna, the unique community structure, and multivariate analyses suggest that the Antarctic vent ecosystems represent a new vent biogeographic province. However, multivariate analyses of species present at the ESR and at other deep-sea hydrothermal vents globally indicate that vent biogeography is more complex than previously recognised. PMID:22235194

Rogers, Alex D.; Tyler, Paul A.; Connelly, Douglas P.; Copley, Jon T.; James, Rachael; Larter, Robert D.; Linse, Katrin; Mills, Rachel A.; Garabato, Alfredo Naveira; Pancost, Richard D.; Pearce, David A.; Polunin, Nicholas V. C.; German, Christopher R.; Shank, Timothy; Boersch-Supan, Philipp H.; Alker, Belinda J.; Aquilina, Alfred; Bennett, Sarah A.; Clarke, Andrew; Dinley, Robert J. J.; Graham, Alastair G. C.; Green, Darryl R. H.; Hawkes, Jeffrey A.; Hepburn, Laura; Hilario, Ana; Huvenne, Veerle A. I.; Marsh, Leigh; Ramirez-Llodra, Eva; Reid, William D. K.; Roterman, Christopher N.; Sweeting, Christopher J.; Thatje, Sven; Zwirglmaier, Katrin

2012-01-01

275

Aspartic acid racemization and amino acid composition of the organic endoskeleton of the deep-water colonial anemone Gerardia : Determination of longevity from kinetic experiments  

NASA Astrophysics Data System (ADS)

An age of 250 (±70) yr is estimated for a specimen of the colonial anemone Gerardia collected alive from 630 m depth off the Bahamas. This age is calculated from (1) the difference in aspartic acid (Asp) racemization measured in the outermost (youngest) and innermost (oldest) layers of the trunk of the organic endoskeleton of the specimen and (2) the estimated rate of Asp racenlization, as extrapolated from heating experiments. A progressive increase in D/L Asp values was observed from the outermost (0.052) to the innermost (0.099) layer of the trunk. Asp racemization rates determined at 60, 80, and 100°C in the laboratory were used to determine the Arrhenius parameters, from which the rate at present ambient temperature (12.5°C) was calculated. The activation energy of Asp racemization was estimated to be 29.1 kcal/mol. The estimated age (250 yr) is much younger than that suggested by radiocarbon measurements of the specimen (ca. 1800 yr). The amino acid composition of the endoskeleton of Gerardia is unusual, with histidine comprising 30% of the total. The composition resembles proteins of the endoskeleton of antipatharians (black corals).

Goodfriend, Glenn A.

1997-05-01

276

Sea Turtle Conservancy  

NSDL National Science Digital Library

The Caribbean Conservation Corporation (CCC) was founded in 1959 by sea turtle champions such as ecologist Dr. Archie Carr, who served as the CCC's Scientific Director for nearly three decades. As the oldest sea turtle organization on the globe, the CCC "works to enact protective laws and establish refuges for the preservation of sea turtle habitats and coastal environments." The CCC created the Sea Turtle Survival League (STSL) in 1993 "as a public education and advocacy program to begin addressing the threats that face U.S. sea turtle populations." The CCC & STSL website contains information about a number of sea turtle programs and projects, tracking sea turtles, different sea turtle species, and ways to become a sea turtle conservationist. CCC also offers a public discussion board, a variety of downloadable publications (including activities for kids), and a collection of related links.

277

Adhesion networks of cnidarians: a postgenomic view.  

PubMed

Cell-extracellular matrix (ECM) and cell-cell adhesion systems are fundamental to the multicellularity of metazoans. Members of phylum Cnidaria were classified historically by their radial symmetry as an outgroup to bilaterian animals. Experimental study of Hydra and jellyfish has fascinated zoologists for many years. Laboratory studies, based on dissection, biochemical isolations, or perturbations of the living organism, have identified the ECM layer of cnidarians (mesoglea) and its components as important determinants of stem cell properties, cell migration and differentiation, tissue morphogenesis, repair, and regeneration. Studies of the ultrastructure and functions of intercellular gap and septate junctions identified parallel roles for these structures in intercellular communication and morphogenesis. More recently, the sequenced genomes of sea anemone Nematostella vectensis, Hydra magnipapillata, and coral Acropora digitifera have opened up a new frame of reference for analyzing the cell-ECM and cell-cell adhesion molecules of cnidarians and examining their conservation with bilaterians. This chapter integrates a review of literature on the structure and functions of cell-ECM and cell-cell adhesion systems in cnidarians with current analyses of genome-encoded repertoires of adhesion molecules. The postgenomic perspective provides a fresh view on fundamental similarities between cnidarian and bilaterian animals and is impelling wider adoption of species from phylum Cnidaria as model organisms. PMID:24411175

Tucker, Richard P; Adams, Josephine C

2014-01-01

278

Evolution and functional diversity of jellyfish opsins.  

PubMed

Cnidaria are the most basal animal phylum possessing complex eyes [1]. Their eyes predominantly use ciliary photoreceptor cells (c-PRCs) like vertebrates, whereas insect eyes use rhabdomeric photoreceptor cells (r-PRCs) [1-4]. These two cell types show not only different cytoarchitectures but distinct phototransduction cascades, which are triggered by the respective types of opsins (e.g., [5]), ciliary opsins (c-opsins) and rhabdomeric opsins (r-opsins) [6]. Recent reports suggested that the c- and r-PRCs and their respective opsins diverged at least before the deuterostome-protostome split [7-9]. To study the earlier evolution of animal PRCs and opsins, we investigated two hydrozoan jellyfishes. We report here the first-characterized cnidarian opsins. Molecular phylogeny revealed that the cloned 20 jellyfish opsins, together with all the opsins from a hydra and some from a sea anemone, are more closely related to the c-opsins than to any other major opsin subfamily, indicating that the divergence of c- and r-opsins antedates the Cnidaria-Bilateria split. Possible scenarios of animal PRC evolution are discussed. Furthermore, Cladonema opsins show several distinct tissue- and stage-specific expression patterns. The expression of specific opsins in the eyes suggests a role in vision, whereas that in the gonads suggests a role in light-controlled release of gametes. PMID:18160295

Suga, Hiroshi; Schmid, Volker; Gehring, Walter J

2008-01-01

279

All That Unplowed Sea  

ERIC Educational Resources Information Center

Hunting and gathering at sea may fast be approaching their productive limits. Aquaculture - farming at sea - linked to conservation represents the sea's promise. If the system works, it might prove to be the key to supplying large amounts of food and fresh water at no cost in nonrenewable energy resources. (BT)

MOSAIC, 1975

1975-01-01

280

Sea Education Association  

NSDL National Science Digital Library

Headquartered in Woods Hole, Massachusetts, SEA offers a variety of science programs at sea for high school and college students. Site features information on the vessels, the crew, current voyages, admissions information, and a wealth of photographs from past expeditions. Also includes a section where you can track the progress of the SEA boats and hear daily, and archived, audio reports.

281

Tracking Sea Otters  

USGS Multimedia Gallery

Sea otter researchers Michelle Staedler, Monterey Bay Aquarium, and Tim Tinker, USGS, work together to locate sea otters in their study project. USGS scientists and their partners study sea otters in efforts to help the threatened species continue to recover from near extinction....

282

``In Forbidden Seas''  

Microsoft Academic Search

``D. W. T.'', who writes a review of the sea-otter, or rather of a book called ``In Forbidden Seas'', in NATURE of January 26, tells us that he is not aware that any living naturalist has ever seen this animal in its natural state. Now, Captain H. J. Snow, who is the author of ``In Forbidden Seas'', is, from my

John Milne

1911-01-01

283

Mapping Sea Level Rise  

NSDL National Science Digital Library

In this activity related to climate change, learners create and explore topographical maps as a means of studying sea level rise. Learners use various everyday materials including ice and a potato to investigate the difference between sea ice and glaciers in relation to sea level rise, create and use a topographical map to predict sea level rise, and discuss how sea level rise will affect Alaska's coastline. This lesson plan includes detailed activity procedure guidelines, critical thinking questions, an overhead, and handouts. NOTE: material cost does not include cost to purchase DVD since it is not essential to the activity.

Fairbanks, University O.

2008-01-01

284

Dust Storm, Aral Sea  

NASA Technical Reports Server (NTRS)

The Aral Sea has shrunk to less than half its size since 1985. The Aral Sea receives little water (sometimes no water) from the two major rivers that empty into it-the Syr Darya and Amu Darya. Instead, the river water is diverted to support irrigation for the region's extensive cotton fields. Recently, water scarcity has increased due to a prolonged drought in Central Asia. As the Aral Sea recedes, its former sea bed is exposed. The Aral's sea bed is composed of fine sediments-including fertilizers and other agricultural chemicals-that are easily picked up by the region's strong winds, creating thick dust storms. The International Space Station crew observed and recorded a large dust storm blowing eastward from the Aral Sea in late June 2001. This image illustrates the strong coupling between human activities (water diversions and irrigation), and rapidly changing land, sea and atmospheric processes-the winds blow across the

2002-01-01

285

Australian Cretaceous Cnidaria and Porifera  

Microsoft Academic Search

Australian Cretaceous sponge and coral faunas are reviewed and increased with new discoveries. The largest new fauna described, from the very thin Maastrichtian Miria Formation, an uncemented chalky marl, in the Carnarvon Basin, Western Australia, includes a poriferan, Ventriculites sp., the hydrozoans, Stylaster cretaceous sp. nov. and Astya nielseni Wells, 1977 originally described from the Eocene of Tonga and the

John S. Jell; Alex G. Cook; Peter A. Jell

2011-01-01

286

Phylogenetics of Hydroidolina (Hydrozoa: Cnidaria)  

E-print Network

Nectadamas diomedeae EU305522 AY937348 AY935306 YPM 35352 Prayidae Nectopyramis sp./N. natans AY026377 AF358068 AY935307 Prayidae Praya dubia EU305526 AY937326 AY935285 YPM 35346 Prayidae Rosacea flaccida EU305529 AY937328 YPM 35041 Physonectae Agalmatidae...

Cartwright, Paulyn; Evans, Nathaniel M.; Dunn, Casey W.; Marques, Antonio C.; Miglietta, Maria Pia; Schuchert, Peter; Collins, Allen G.

2008-05-08

287

Numerical increases and distributional shifts of Chrysaora quinquecirrha (Desor) and Aurelia aurita (Linné) (Cnidaria: Scyphozoa) in the northern Gulf of Mexico  

Microsoft Academic Search

Fisheries resource trawl survey data from the National Marine Fisheries Service from a 11–13-year period to 1997 were examined to quantify numerical and distributional changes of two species of northern Gulf of Mexico scyphomedusae: the Atlantic sea nettle, Chrysaora quinquecirrha (Desor), and the moon jelly, Aurelia aurita (Linné). Trawl surveys were grouped into 10 statistical regions from Mobile Bay, Alabama

W. M. Graham

2001-01-01

288

the East Sea Sea of Japan  

Microsoft Academic Search

Sediment cores collected from the deep basins of the East Sea Sea of Japan provide an ongoing and historical record of artificial radionuclides contamination into one of the most highly publicized radioactive waste dumping areas in the world ocean. The depth distributions of 90Sr, 137Cs and 239,240Pu in sediment cores were investigated with 210 . the aid of Pb-derived sediment

Gi-Hoon Hong; Sang-Han Lee; Suk-Hyun Kim; Chang-Soo Chung; M. Baskaran

289

THE SEALS, SEA-LIONS, AND SEA OTTER OF  

E-print Network

THE SEALS, SEA-LIONS, AND SEA OTTER OF THE PACIFIC COAST Marine Biological Laboratory OODS HOLE, SEA-LIONS, AND SEA OTTER OF THE PACIFIC COAST Descriptions, Life History Notes, Photographs, and sea otter of the Pacific from Mexico to Point Barrow and the Hawaiian Islands. For each of twelve

290

Power from the sea  

Microsoft Academic Search

The feasibility of sea thermal power systems exploiting the temperature difference between water at the surface and in the depths of tropical seas is examined. It has been estimated that the cost of electricity from sea thermal plants based on projected 1985 prices may be as low as 25 mills\\/kW hr, in contrast with about 37 mills\\/kW hr for nuclear

M. Swann

1976-01-01

291

Focus on Sea Otters  

NSDL National Science Digital Library

A Monterey Bay Aquarium website where you can learn about the biology and population recovery of sea otters. Features include the opportunity to "meet" the otters on exhibit at the aquarium and viewing them through the live otter cam. Many sea otter-related games, activities, and resources. Links to other fascinating exhibits at the Aquarium. Several downloadable videos available, each with their own enjoyable sea otter antics.

292

Evolution of the tetraploid Anemone multifida (2n = 32) and hexaploid A. baldensis (2n = 48) (Ranunculaceae) was accompanied by rDNA loci loss and intergenomic translocation: evidence for their common genome origin  

PubMed Central

Background and Aims In the genus Anemone two small groups of taxa occur with the highest ploidy levels 2n = 6x = 48, belonging to the closely related clades: the montane/alpine Baldensis clade and the more temperate Multifida clade. To understand the formation of polyploids within these groups, the evolution of allohexaploid A. baldensis (AABBDD, 2n = 6x = 48) from Europe and allotetraploid Anemone multifida (BBDD, 2n = 4x = 32) from America was analysed. Methods Internal transcribed spacer and non-transcribed spacer sequences were used as molecular markers for phylogenetic analyses. Cytogenetic studies, including genomic in situ hybridization with genomic DNA of potential parental species as probe, fluorescence in situ hybridization with 5S and 18S rDNA as probes and 18S rDNA restriction analyses, were used to identify the parental origin of chromosomes and to study genomic changes following polyploidization. Key Results This study shows that A. multifida (BBDD, 2n= 4x = 32) and A. baldensis (AABBDD, 2n = 6x = 48) are allopolyploids originating from the crosses of diploid members of the Multifida (donor of the A and B subgenomes) and Baldensis groups (donor of the D subgenome). The A and B subgenomes are closely related to the genomes of A. sylvestris, A. virginiana and A. cylindrica, indicating that these species or their progeny might be the ancestral donors of the B subgenome of A. multifida and A and B subgenomes of A. baldensis. Both polyploids have undergone genomic changes such as interchromosomal translocation affecting B and D subgenomes and changes at rDNA sites. Anemone multifida has lost the 35S rDNA loci characteristic of the maternal donor (B subgenome) and maintained only the rDNA loci of the paternal donor (D subgenome). Conclusions It is proposed that A. multifida and A. baldensis probably had a common ancestor and their evolution was facilitated by vegetation changes during the Quaternary, resulting in their present disjunctive distribution. PMID:22711694

Mlinarec, J.; Šatovi?, Z.; Malenica, N.; Ivan?i?-Ba?e, I.; Besendorfer, V.

2012-01-01

293

Sea level extremes in the Caribbean Sea  

NASA Astrophysics Data System (ADS)

level extremes in the Caribbean Sea are analyzed on the basis of hourly records from 13 tide gauges. The largest sea level extreme observed is 83 cm at Port Spain. The largest nontidal residual in the records is 76 cm, forced by a category 5 hurricane. Storm surges in the Caribbean are primarily caused by tropical storms and stationary cold fronts intruding the basin. However, the seasonal signal and mesoscale eddies also contribute to the creation of extremes. The five stations that have more than 20 years of data show significant trends in the extremes suggesting that flooding events are expected to become more frequent in the future. The observed trends in extremes are caused by mean sea level rise. There is no evidence of secular changes in the storm activity. Sea level return periods have also been estimated. In the south Colombian Basin, where large hurricane-induced surges are rare, stable estimates can be obtained with 30 years of data or more. For the north of the basin, where large hurricane-induced surges are more frequent, at least 40 years of data are required. This suggests that the present data set is not sufficiently long for robust estimates of return periods. ENSO variability correlates with the nontidal extremes, indicating a reduction of the storm activity during positive ENSO events. The period with the highest extremes is around October, when the various sea level contributors' maxima coincide.

Torres, R. Ricardo; Tsimplis, Michael N.

2014-08-01

294

Pelagic sea snakes dehydrate at sea.  

PubMed

Secondarily marine vertebrates are thought to live independently of fresh water. Here, we demonstrate a paradigm shift for the widely distributed pelagic sea snake, Hydrophis (Pelamis) platurus, which dehydrates at sea and spends a significant part of its life in a dehydrated state corresponding to seasonal drought. Snakes that are captured following prolonged periods without rainfall have lower body water content, lower body condition and increased tendencies to drink fresh water than do snakes that are captured following seasonal periods of high rainfall. These animals do not drink seawater and must rehydrate by drinking from a freshwater lens that forms on the ocean surface during heavy precipitation. The new data based on field studies indicate unequivocally that this marine vertebrate dehydrates at sea where individuals may live in a dehydrated state for possibly six to seven months at a time. This information provides new insights for understanding water requirements of sea snakes, reasons for recent declines and extinctions of sea snakes and more accurate prediction for how changing patterns of precipitation might affect these and other secondarily marine vertebrates living in tropical oceans. PMID:24648228

Lillywhite, Harvey B; Sheehy, Coleman M; Brischoux, François; Grech, Alana

2014-05-01

295

Hawksbill Sea Turtle (Eretmochelys imbricata)  

USGS Multimedia Gallery

Hawksbill Sea Turtles (Eretmochelys imbricata), such as the one hiding here under a boulder, and Green Sea Turtles (Chelonia mydas) are occasionally seen in Hurricane Hole. Hawksbills feed mostly on sponges while Greens eat mostly sea grasses....

296

White Sea - Russia  

NASA Technical Reports Server (NTRS)

At bottom center of this true-color Moderate Resolution Imaging Spectroradiometer (MODIS) image from April 13, 2001, the White Sea in western Russia is becoming free of ice in its southern extent. Meanwhile, the blue-green waters along the coast of the peninsula jutting out into the Barents Sea to the northeast could be due to a phytoplankton bloom.

2002-01-01

297

Sea Lion Skeleton - Skull  

NSDL National Science Digital Library

The carnivorous sea lion uses its sharp pointed teeth and large mouth to shred and tear its prey. The large nose and large eyes on either side of the skull help the sea lion to detect prey. The skull protects the brain from damage and injury.

Ketan Patel (California State University, Fullerton;Student, Biological Sciences)

2007-07-26

298

Sea Lion Skeleton - Nostrils  

NSDL National Science Digital Library

The carnivorous sea lion uses its sharp pointed teeth and large mouth to shred and tear its prey. The large nose and large eyes on either side of the skull help the sea lion to detect prey. The skull protects the brain from damage and injury.

Ketan Patel (California State University, Fullerton;Student, Biological Sciences)

2007-07-26

299

Sea bed mechanics  

Microsoft Academic Search

This book provides a discussion on sea bed processes with engineering applications. It brings together the material currently available only in technical reports of research papers. It provides formulae and background references necessary for design calculation of problems such as sea bed or coastal erosion, and sub-marine pipeline stability. It also covers dissipation of wave energy, formation of ripples and

Sleath

1984-01-01

300

Spotting Sea Otters  

USGS Multimedia Gallery

USGS wildlife biologist Alisha Kage looks through a telescope to help her locate and identify tagged sea otters, then records the otter's location for a study aimed at learning more about the species. USGS scientists study sea otters in efforts to help the threatened species continue to recover from...

301

Tracking Sea Otters  

USGS Multimedia Gallery

USGS wildlife biologist Alisha Kage holds out a VHF receiver, hoping to hear the tell-tale beep that helps her locate sea otters that are part of study to monitor and learn more about the species. USGS scientists study sea otters in efforts to help the threatened species continue to recover from nea...

302

Sea Otter Unit  

NSDL National Science Digital Library

Teacher's manual on sea otters with lesson plans on: using the web as a research tool; reading and developing writing skills; social studies with role playing activity in community conflict resolution. Includes a glossary, links to additional resources, and background materials on sea otter history, distribution, vital statistics, behavior, role in food web, threats and conservation measures. Suggests conservation activities for classroom or school.

303

Kara Sea radioactivity assessment  

Microsoft Academic Search

Investigations following five international expeditions to the Kara Sea have shown that no radiologically significant contamination has occurred outside of the dumping sites in Novaya Zemlya bays. Increased levels of radionuclides in sediment have only been observed in Abrosimov and Stepovoy Bays very close to dumped containers. Evaluations of radionuclide inventories in water and sediment of the open Kara Sea

Iolanda Osvath; Pavel P Povinec; Murdoch S Baxter

1999-01-01

304

Red sea drillings.  

PubMed

Recent drilling in the Red Sea has shown that much of the basin is underlain by evaporites of a similar age to that of evaporites found in the Mediterranean Sea. These evaporites and their structural positions indicate that other brine areas are present-and, indeed, several others have been discovered. PMID:17843766

Ross, D A; Whitmarsh, R B; Ali, S A; Boudreaux, J E; Coleman, R; Fleisher, R L; Girdler, R; Manheim, F; Matter, A; Nigrini, C; Stoffers, P; Supko, P R

1973-01-26

305

Black Sea in Bloom  

NASA Technical Reports Server (NTRS)

This true-color image shows bright, turquoise-colored swirls across the surface of the Black Sea, signifying the presence of a large phytoplankton bloom. Scientists have observed similar blooms recurring annually, roughly this same time of year. The Sea of Azov, which is the smaller body of water located just north of the Black Sea in this image, also shows a high level of biological activity currently ongoing. The brownish pixels in the Azov are probably sediments carried in from high waters upstream. This scene was acquired by the Sea-viewing Wide Field-of-view Sensor (SeaWiFS), flying aboard the OrbView-2 satellite, on May 4, 2002. According to the Black Sea Environment Programme's Marine Hydrophysical Institute, the Black Sea is 'one of the marine areas of the world most damaged by human activities.' The coastal zone around these Eastern European inland water bodies is densely populated-supporting a permanent population of roughly 16 million people and another 4 million tourists each year. Six countries border with the Black Sea, including Ukraine to the north, Russia and Georgia to the east, Turkey to the south, and Bulgaria and Romania to the west. Because it is isolated from the world's oceans, and because there is an extensive drainage network of rivers that empty into it, the Black Sea has a unique and delicate water balance which is very important for supporting its marine ecosystem. Of particular concern to scientists is the salinity, water level, and nutrient levels of the Black Sea's waters, all of which are, unfortunately, being impacted by human activities. Within the last three decades the combination of increased nutrient loads from human sources together with pollution and over-harvesting of fisheries has resulted in a sharp decline in water quality. Scientists from each of the Black Sea's bordering nations are currently working together to study the issues and formulate a joint, international strategy for saving this unique marine ecosystem. Working with a spirit of placing more emphasis on joint ownership of the Black Sea's resources, and less emphasis on blame, it is hoped that the cooperating countries can strike an effective balance between both enjoying and preserving the Black Sea. Image courtesy the SeaWiFS Project, NASA GSFC, and ORBIMAGE

2002-01-01

306

Sea Cucumbers (Holothuroidea)  

NSDL National Science Digital Library

This Report's Topic in Depth explores the world of sea cucumbers, or Holothuroidea, a diverse group of intriguing marine animals. The first site (1), from the Tree of Life Web Project, provides nice clear images of sea cucumbers and brief concise sections on Characteristics, The Orders of Holothuroidea, Fossil History, and Discussion of Phylogenetic Relationships. The second site (2), from the Charles Darwin Research Station, displays short answers to commonly asked questions about sea cucumbers like: What is their importance within the marine communities?; How do sea cucumbers reproduce?; and What is the potential environment impact of overexploiting sea cucumber populations? From Enchanted Learning, the third site (3) features a diagrammed print-out of a s ea cucumber along with short descriptions of Holothuroidea anatomy, diet, classification, and predators. Hosted by the Royal BC Museum, the fourth site (4) contains a brief research paper by curator Philip Lambert on taxonomy issues concerning sea cucumbers. The fifth site (5), developed by Richard Fox of Lander University, contains detailed instructions for a laboratory exercise with Sclerodactyla briareus, a species of sea cucumber. From MoonDragon's Health & Wellness website, the sixth site (6) contains a sea cucumber recipe and briefly discusses sea cucumber cuisine and health benefits. Hosted by the Secretariat of the Pacific Community, the seventh site (7) provides information about an international conference titled: Conservation of s ea cucumbers in Malaysia, their Taxonomy, Ecology and Trade. The site contains concluding remarks, abstracts from papers presented at the conference, and a list of email contacts for conference participants. The final (8) site from the Environmental News Network'features a short article about an Ecuadorian court upholding sea cucumber fishing limits in the Galapagos islands.

307

Semi-Automated Image Analysis for the Assessment of Megafaunal Densities at the Arctic Deep-Sea Observatory HAUSGARTEN  

PubMed Central

Megafauna play an important role in benthic ecosystem function and are sensitive indicators of environmental change. Non-invasive monitoring of benthic communities can be accomplished by seafloor imaging. However, manual quantification of megafauna in images is labor-intensive and therefore, this organism size class is often neglected in ecosystem studies. Automated image analysis has been proposed as a possible approach to such analysis, but the heterogeneity of megafaunal communities poses a non-trivial challenge for such automated techniques. Here, the potential of a generalized object detection architecture, referred to as iSIS (intelligent Screening of underwater Image Sequences), for the quantification of a heterogenous group of megafauna taxa is investigated. The iSIS system is tuned for a particular image sequence (i.e. a transect) using a small subset of the images, in which megafauna taxa positions were previously marked by an expert. To investigate the potential of iSIS and compare its results with those obtained from human experts, a group of eight different taxa from one camera transect of seafloor images taken at the Arctic deep-sea observatory HAUSGARTEN is used. The results show that inter- and intra-observer agreements of human experts exhibit considerable variation between the species, with a similar degree of variation apparent in the automatically derived results obtained by iSIS. Whilst some taxa (e. g. Bathycrinus stalks, Kolga hyalina, small white sea anemone) were well detected by iSIS (i. e. overall Sensitivity: 87%, overall Positive Predictive Value: 67%), some taxa such as the small sea cucumber Elpidia heckeri remain challenging, for both human observers and iSIS. PMID:22719868

Schoening, Timm; Bergmann, Melanie; Ontrup, Jörg; Taylor, James; Dannheim, Jennifer; Gutt, Julian; Purser, Autun; Nattkemper, Tim W.

2012-01-01

308

Molecular phylogenetic evidence for the evolution of specialization in anemonefishes  

Microsoft Academic Search

Anemone¢shes (genera: Amphiprion and Premnas; family Pomacentridae) are a group of 28 species of coral reef ¢shes that are found in obligate symbiosis with large tropical sea anemones. A phylogenetic hypoth- esis based on morphological analyses of this group suggests that the ancestral anemone¢sh was a generalist with similar morphology to other pomacentrids, and that it gave rise to other

J. K. Elliott; S. C. Lougheed; B. Bateman; L. K. McPhee; P. T. Boag

1999-01-01

309

SEAS Safety Program SEAS SAFETY PROGRAM 2012-2103  

E-print Network

Safety Administrative Support · Linn Eichler & Christie Gilliland Safety Inspection Support · Lab Safety #12;SEAS Safety ProgramSEAS Safety Program Services Provided to SEAS Research Labs · Annual Lab Safety · Relay relevant information back to your lab group · Report incidents to SEAS safety committee · Attend

310

Global sea level rise  

SciTech Connect

Published values for the long-term, global mean sea level rise determined from tide gauge records exhibit considerable scatter, from about 1 mm to 3 mm/yr. This disparity is not attributable to instrument error; long-term trends computed at adjacent sites often agree to within a few tenths of a millimeter per year. Instead, the differing estimates of global sea level rise appear to be in large part due to authors' using data from gauges located at convergent tectonic plate boundaries, where changes of land elevation give fictitious sea level trends. In addition, virtually all gauges undergo subsidence or uplift due to postglacial rebound (PGR) from the last deglaciation at a rate comparable to or greater than the secular rise of sea level. Modeling PGR by the ICE-3G model of Tushingham and Peltier (1991) and avoiding tide gauge records in areas of converging tectonic plates produces a highly consistent set of long sea level records. The value for mean sea level rise obtained from a global set of 21 such stations in nine oceanic regions with an average record length of 76 years during the period 1880-1980 is 1.8 mm/yr {plus minus} 0.1. This result provides confidence that carefully selected long tide gauge records measure the same underlying trend of sea level and that many old tide gauge records are of very high quality.

Douglas, B.C. (NOAA, Rockville, MD (USA))

1991-04-15

311

Sea level variation  

NASA Technical Reports Server (NTRS)

Published values for the long-term, global mean sea level rise determined from tide gauge records range from about one to three mm per year. The scatter of the estimates appears to arise largely from the use of data from gauges located at convergent tectonic plate boundaries where changes of land elevation give fictitious sea level trends, and the effects of large interdecadal and longer sea level variations on short (less than 50+ years) or sappy records. In addition, virtually all gauges undergo subsidence or uplift due to isostatic rebound from the last deglaciation at a rate comparable to or greater than the secular rise of sea level. Modeling rebound by the ICE-3G model of Tushingham and Peltier (1990) and avoiding tide gauge records in areas of converging tectonic plates produces a highly consistent set of long sea level records. A global set of 21 such stations in nine oceanic regions with an average record length of 76 years during the period 1880-1980 yields the global sea level rise value 1.8 mm/year +/- 0.1. Greenhouse warming scenarios commonly forecast an additional acceleration of global sea level in the next 5 or 6+ decades in the range 0.1-0.2 mm/yr2. Because of the large power at low frequencies in the sea level spectrum, very long tide gauge records (75 years minimum) have been examined for past apparent sea level acceleration. For the 80-year period 1905-1985, 23 essentially complete tide gauge records in 10 geographic groups are available for analysis. These yielded the apparent global acceleration -0.011 (+/- 0.012) mm/yr2. A larger, less uniform set of 37 records in the same 10 groups with 92 years average length covering the 141 years from 1850-1991 gave 0.001 (+/- 0.008) mm/yr2. Thus there is no evidence for an apparent acceleration in the past 100+ years that is significant either statistically, or in comparison to values associated with global warming. Unfortunately, the large interdecadal fluctuations of sea level severely affect estimates of global sea level acceleration for time spans of less than about 50 years. This means that tide gauges alone cannot serve as a reliable leading indicator of climate change in less than many decades. This time required can be significantly reduced if the interdecadal fluctuations of sea level can be understood in terms of their forcing mechanisms, and then removed from the tide gauge records.

Douglas, Bruce C.

1992-01-01

312

Arctic Sea Ice  

NSDL National Science Digital Library

In this activity, learners explore how the area of Arctic sea ice has changed over recent years. First, learners graph the area of Arctic sea ice over time from 1979 to 2007. Then, learners use this information to extrapolate what the area will be in 2018 and graph their predictions. In part two of the activity, learners make a flip book to simulate the sea changes they just graphed. This resource includes background information related to the Northwest Passage and questions for learners to answer after completing this activity.

Meier, Beverly L.

2012-06-26

313

Sea Perch Project  

NASA Technical Reports Server (NTRS)

David Lalejini, an employee of the Naval Research Laboratory at NASA's John C. Stennis Space Center, helps a pair of teachers deploy a remotely-operated underwater Sea Perch robot during workshop activities Dec. 11. The Stennis Education Office teamed with Naval Research Laboratory counterparts to conduct a two-day workshop Dec. 10-11 for Louisiana and Mississippi teachers. During the no-cost workshop, teachers learned to build and operate Sea Perch robots. The teachers now can take the Sea Perch Program back to students.

2010-01-01

314

Is The Sea Level?  

NSDL National Science Digital Library

In this activity students will observe that the sea level changes and will hypothesize what causes this change. They will then check their hypothesis with a data set. Many students are surprised to learn that sea level is not the same everywhere on earth and that it changes with the seasons. The main cause of this change is the temperature change in the ocean - warmer waters are higher than colder waters. Students will discover this information as they complete the activity and then see if the temperature effect holds true on another data set showing temperature and sea height changes caused by the El Nino/Southern Oscillation (ENSO).

2007-12-12

315

Drag of the sea surface  

Microsoft Academic Search

It is shown how the drag of the sea surface can be computed from the wind speed and the sea state. The approach, applicable both for fully developed and for developing seas, is based on conservation of momentum in the boundary layer above the sea, which allows one to relate the drag to the properties of the momentum exchange between

V. K. Makin; V. N. Kudryavtsev; C. Mastenbroek

1995-01-01

316

3. The Sea Urchin Introduction  

E-print Network

3. The Sea Urchin leffHardin Introduction The sea urchin embryo has been used for more than of echinoderms and, in particular, of sea urchins, that was carried out at these marine stations was influential; Morgan, 1927). Later, in the early part of the twentieth century, the experiments performed on sea urchin

Hardin, Jeff

317

Epibenthic assemblages in the Celtic Sea and associated with the Jones Bank  

NASA Astrophysics Data System (ADS)

The epibenthic assemblages in the Celtic Sea are described from the catches from 2 m beam trawl surveys undertaken from 2000 to 2009. During this period 154 samples were collected. The most ubiquitous species in the study area were the natantid shrimps Processa spp. and Crangon allmanni, the hermit crabs Pagurus prideaux and Anapagurus laevis, sand star Astropecten irregularis and spotted dragonet Callionymus maculatus. Multivariate community analyses indicated that catches (numbers per tow) were distributed across six assemblages, two of which were predominant in the study area. Most catches were attributed to either a 'shelf edge assemblage', which was widespread in deeper waters (114-423 m water depth) or an 'outer shelf assemblage' that occurred across much of the Celtic Sea north of 49°N in waters 49-175 m deep. The dominant species along the edge of the continental shelf were the hormathid anemome Actinauge richardi, sea spider Pycnogonum littorale (which associated with A. richardi), Devonshire cup coral Caryophyllia smithii and the swimming crab Macropipus tuberculatus. The dominant species in the outer shelf assemblage included P. prideaux, C. allmanni, A. laevis and common starfish Asterias rubens. Stations closer to shore were relatively distinct and catches in this 'inner shelf assemblage' were composed primarily of an inshore fauna (e.g. Ophiura ophiura, C. allmanni and Liocarcinus holsatus). Stations in the southern part of the survey grid were also relatively distinct ('southern Celtic Sea assemblage'), and several large echinoderms (Porania pulvillus, Stichastrella rosea and Anseropoda placenta) dominated at these sites. Three of the deepest stations were also relatively distinct, as were a group of stations in the muddy habitat of the Celtic Deep and comparable grounds elsewhere in the region, where Nucula sulcata and Alpheus glaber were characteristic. Catches on the shallower parts of the Jones Bank (and on another bank in the region) were dominated by the anemone Paraphellia expansa, with off-bank sites comprising a greater number of species. In contrast to beam trawl sampling, baited camera observations on the Jones Bank showed a greater richness of species on the shallower part of the bank, and provided information on the nocturnal feeding behaviour of scavenging isopods.

Ellis, J. R.; Martinez, I.; Burt, G. J.; Scott, B. E.

2013-10-01

318

Processes driving sea ice variability in the Bering Sea in an eddying ocean/sea ice model: Mean seasonal cycle  

E-print Network

Processes driving sea ice variability in the Bering Sea in an eddying ocean/sea ice model: Mean 17 September 2014 Available online 28 September 2014 Keywords: Sea ice Ice growth/melt Sea ice motion Heat flux Climate dynamics Bering Sea a b s t r a c t The seasonal cycle of sea ice variability

Eisenman, Ian

319

Sea level change  

SciTech Connect

The IPCC (Intergovernmental Panel on Climate Change) 1995 Scientific Assessment, Chapter 7. Sea Level Change, presents a modest revision of the similar chapter in the 1990 Assessment. Principal conclusions on observed sea-level change and the principal terms in the sea-level equation (ocean thermal expansion, glaciers, ice sheets, and land hydrology), including our knowledge of the present-day (defined as the 20th Century) components of sea-level rise, and projections of these for the future, are presented here. Some of the interesting glaciological problems which are involved in these studies are discussed in more detail. The emphasis here is on trends over decades to a century, not on shorter variations nor on those of the geologic past. Unfortunately, some of the IPCC projections had not been agreed at the time of writing of this paper, and these projections will not be given here. 15 refs., 2 figs.

Meier, M.F. [Univ. of Colorado, Boulder, CO (United States)

1996-12-31

320

Purple sea urchin swarm  

NSDL National Science Digital Library

Sea urchins live in low tide regions and eat seaweed. Urchins have no arms but have five rows of tube feet for movement. They are found in holes and use their spines for protection and to burrow into the rocks.

Katie Hale (California State University, Fullerton;Student, Biological Sciences)

2007-01-04

321

Smart Sea Lions  

NSDL National Science Digital Library

This video segment adapted from NOVA scienceNOW explores whether animals and humans are more similar than we think. Meet Rio, a sea lion who demonstrates to researchers reasoning skills once thought limited to humans.

WGBH Educational Foundation

2009-08-31

322

Sea Floor Spreading I  

NSDL National Science Digital Library

In this introductory Excel tutorial (Activity I) students use Excel to explore the geodynamics model equation for ocean depth around a sea-floor spreading center. For students with no prior Excel experience.

Activity and Starting Point page by R.M. MacKay. Clark College, Physics and Meteorology.

323

Sea Ice Ecosystems  

NASA Astrophysics Data System (ADS)

Polar sea ice is one of the largest ecosystems on Earth. The liquid brine fraction of the ice matrix is home to a diverse array of organisms, ranging from tiny archaea to larger fish and invertebrates. These organisms can tolerate high brine salinity and low temperature but do best when conditions are milder. Thriving ice algal communities, generally dominated by diatoms, live at the ice/water interface and in recently flooded surface and interior layers, especially during spring, when temperatures begin to rise. Although protists dominate the sea ice biomass, heterotrophic bacteria are also abundant. The sea ice ecosystem provides food for a host of animals, with crustaceans being the most conspicuous. Uneaten organic matter from the ice sinks through the water column and feeds benthic ecosystems. As sea ice extent declines, ice algae likely contribute a shrinking fraction of the total amount of organic matter produced in polar waters.

Arrigo, Kevin R.

2014-01-01

324

Record Sea Ice Minimum  

NASA Technical Reports Server (NTRS)

Arctic sea ice reached a record low in September 2007, below the previous record set in 2005 and substantially below the long-term average. This image shows the Arctic as observed by the Advanced Microwave Scanning Radiometer for EOS (AMSR-E) aboard NASA's Aqua satellite on September 16, 2007. In this image, blue indicates open water, white indicates high sea ice concentration, and turquoise indicates loosely packed sea ice. The black circle at the North Pole results from an absence of data as the satellite does not make observations that far north. Three contour lines appear on this image. The red line is the 2007 minimum, as of September 15, about the same time the record low was reached, and it almost exactly fits the sea ice observed by AMSR-E. The green line indicates the 2005 minimum, the previous record low. The yellow line indicates the median minimum from 1979 to 2000.

2007-01-01

325

Stellar Sea Lion Research  

NSDL National Science Digital Library

The phenomenon is the decline in population of western Stellar Sea Lions from 1969 to 1986, shown in a series of three images. The accompanying text describes the possible factors that may be contributing to the change in population.

326

Black Sea Becomes Turquoise  

NASA Technical Reports Server (NTRS)

This true-color image shows bright, turquoise-colored swirls across the surface of the Black Sea, signifying the presence of a large phytoplankton bloom. Scientists have observed similar blooms recurring annually, roughly this same time of year. The Sea of Azov, which is the smaller body of water located just north of the Black Sea in this image, also shows a high level of color variance. The brownish pixels in the Azov are probably due to sediments carried in from high waters and snowmelt from upstream. This scene was acquired by the Moderate Resolution Imaging Spectroradiometer, flying aboard NASA's Terra satellite, on May 14, 2002. According to the Black Sea Environment Programme's Marine Hydrophysical Institute, the Black Sea is ?one of the marine areas of the world most damaged by human activities.? The coastal zone around these Eastern European inland water bodies is densely populated'supporting a permanent population of roughly 16 million people and another 4 million tourists each year. Six countries border with the Black Sea, including Ukraine to the north, Russia and Georgia to the east, Turkey to the south, and Bulgaria and Romania to the west. Because it is isolated from the world's oceans, and because there is an extensive drainage network of rivers that empty into it, the Black Sea has a unique and delicate water balance which is very important for supporting its marine ecosystem. Of particular concern to scientists is the salinity, water level, and nutrient levels of the Black Sea's waters, all of which are, unfortunately, being impacted by human activities. Within the last three decades the combination of increased nutrient loads from human sources together with pollution and over-harvesting of fisheries has resulted in a sharp decline in water quality. Scientists from each of the Black Sea's bordering nations are currently working together to study the issues and formulate a joint, international strategy for saving this unique marine ecosystem. Working with a spirit of placing more emphasis on joint ownership of the Black Sea's resources, and less emphasis on blame, it is hoped that the cooperating countries can strike an effective balance between both enjoying and preserving the Black Sea.

2002-01-01

327

Egypt and Red Sea  

NASA Technical Reports Server (NTRS)

A panaramic view of eastern Egypt, The Red Sea and Saudi Arabia beyond (24.0N, 33.0E). In this desert country, where water is life, the high Aswan Dam and the impounded waters of the Nile River in the foreground assure water availability into the next century. The Red Sea beyond, part of the Suez Canal seaway, serves as a commercial link to the world and separates Egypt from Saudi Arabia.

1982-01-01

328

Global sea level rise  

Microsoft Academic Search

Published values for the long-term, global mean sea level rise determined from tide gauge records exhibit considerable scatter, from about 1 mm to 3 mm\\/yr. This disparity is not attributable to instrument error; long-term trends computed at adjacent sites often agree to within a few tenths of a millimeter per year. Instead, the differing estimates of global sea level rise

Bruce C. Douglas

1991-01-01

329

Sea Level rise contributors  

NSDL National Science Digital Library

This web page from the National Snow and Ice Data Center contains two related visualizations. The first visualization gives an estimate of the percent contribution to sea level change since the 1990s from three contributors - small glaciers and ice caps, the Greenland Ice Sheet and the Antarctic Ice Sheet. The second visualization shows the cumulative contribution to sea level from small glaciers and ice caps plotted with the annual global surface air temperature anomaly.

Meier, M. F.; Dyurgerov, Mark; Center, National S.

330

All About Sea Ice  

NSDL National Science Digital Library

This comprehensive site is an introduction to sea ice: what it is, how it forms, how it is studied, how it affected historical expedition in the polar regions, and what role it plays in the global climate. The site contains a glossary of sea ice terms and references to additional information, which all serve as an excellent introduction. Data are also available from various collection methods for student interpretation.

331

Sea Slug Forum  

NSDL National Science Digital Library

Scientists, students, divers and aquarists can ask questions, post information, look up species profiles, references and links about nudibranchs, bubble-shells, sea hares and other sea slugs worldwide. Messages archived by scientific name; species profiles are presented by family and scientific name, and include classification, distribution, biological information and photo, plus links to messages concerning that species. General topics section covers anatomy, behavior, aquarium suitability, and more. Fairly technical.

332

Green Sea Turtles  

NSDL National Science Digital Library

Detailed information on the biology, natural history, factors influencing the population, and protection measures of green sea turtles (Chelonia mydas). Included are three in-class activities and one field activity all designed to raise awareness of the green sea turtle and what humans are doing to affect the population. The site is geared towards the Hawaiian sub-species, however, most of the facts and activities are applicable elsewhere.

333

Sea Level Viewer  

NSDL National Science Digital Library

This website presents ocean topography as measured by sea surface heights taken from space by NASA and CNES. Ocean heat circulation impacts weather and causes events such as Large El Nino, Hurricane Katrina, Indian Ocean Tsunami and La Nina. These events and the latest view of sea surface height are depicted with this 3D interactive viewer. Objectives of NASA missions TOPEX/Poseiden, Jason 1, and Jason 2/OSTM are charted. Closed Captioning is available.

334

Sea Urchin Embryology  

NSDL National Science Digital Library

Advanced high school level laboratory activities using sea urchins to observe fertilization and early developmental stages. This is a comprehensive site complete with multiple labs, support lessons, background information, animated graphics illustrating lab techniques, printable overheads (also available in Spanish and French), and a glossary of terms. A one-stop site for sea urchin information, experiments, suppliers, and research. Links to additional resources are available.

335

National Sea Grant Library  

NSDL National Science Digital Library

Only facility housing complete collection of Sea Grant funded work. An archive and lending library for reprints, books, reports, maps, newsletters, handbooks, videos, CD-roms and computer programs regarding: oceanography; marine education; aquaculture; fisheries; limnology; coastal zone management; marine recreation and law. Lends documents worldwide, aiding scientists, teachers, students, fishermen and others in research and study. Bibliographic database is searchable from the website, obtain citations, abstracts and over 20,000 downloadable texts of Sea Grant publications.

336

The genesis of sea level variability in the Barents Sea  

NASA Astrophysics Data System (ADS)

The regional variability of sea level is an integral indicator of changing oceanographic conditions due to different processes of oceanic, atmospheric, and terrestrial origin. The present study explores the nature of sea level variability in the Barents Sea-a marginal shelf sea of the Arctic Ocean. A characteristic feature that distinguishes this sea from other Arctic shelf seas is that it is largely ice free throughout the year. This allows continuous monitoring of sea level by space-borne altimeters. In this work we combine satellite altimetry, ocean gravity measurements by GRACE satellites, available hydrography data, and a high-resolution ocean data synthesis product to estimate the steric and mass-related components of sea level in the Barents Sea. We present one of the first observational evidence of the local importance of the mass-related sea level changes. The observed 1-3 month phase lag between the annual cycles of sea level in the Barents Sea and in the Nordic seas (Norwegian, Iceland, Greenland seas) is explained by the annual mass-related changes. The analysis of the barotropic vorticity budget shows that the mass-related sea level variability in the central part of the Barents Sea is determined by the combined effect of wind stress, flow over the varying bottom topography, and dissipation, while the impact of vorticity fluxes is negligible. Overall, the steric sea level has smaller amplitudes and mainly varies on the seasonal time scale. The thermosteric sea level is the main contributor to the steric sea level along the pathways of the Atlantic inflow into the Barents Sea. The relative contribution of the halosteric sea level is dominant in the southeastern, eastern, and northern parts of the Barents Sea, modulated by the seasonal sea ice formation/melt as well as by continental runoff. The variability of the thermosteric sea level in the Barents Sea is mostly driven by variations in the net surface heat flux, whereas the contribution of heat advection becomes as important as the ocean-atmosphere heat exchange at interannual time scales.

Volkov, Denis L.; Landerer, Felix W.; Kirillov, Sergey A.

2013-09-01

337

Sea level variations in the Mediterranean Sea and Black Sea from satellite altimetry and tide gauges  

Microsoft Academic Search

Present-day sea level changes in the Mediterranean Sea and Black Sea are studied using satellite altimetry. Analysis of altimetry data from Topex–Poseidon (T\\/P) between January 1993 and December 1998, and from ERS-1\\/2 between October 1992 and June 1996 shows that the mean rate of sea level rise is 7±1.5 mm\\/year over the Mediterranean Sea and 27±2.5 mm\\/year over the Black

A. Cazenave; P. Bonnefond; F. Mercier; K. Dominh; V. Toumazou

2002-01-01

338

The Dead Sea  

NASA Technical Reports Server (NTRS)

The Dead Sea is the lowest point on Earth at 418 meters below sea level, and also one of the saltiest bodies of water on Earth with a salinity of about 300 parts-per-thousand (nine times greater than ocean salinity). It is located on the border between Jordan and Israel, and is fed by the Jordan River. The Dead Sea is located in the Dead Sea Rift, formed as a result of the Arabian tectonic plate moving northward away from the African Plate. The mineral content of the Dead Sea is significantly different from that of ocean water, consisting of approximately 53% magnesium chloride, 37% potassium chloride and 8% sodium chloride. In the early part of the 20th century, the Dead Sea began to attract interest from chemists who deduced that the Sea was a natural deposit of potash and bromine. From the Dead Sea brine, Israel and Jordan produce 3.8 million tons potash, 200,000 tons elemental bromine, 45,000 tons caustic soda, 25, 000 tons magnesium metal, and sodium chloride. Both countries use extensive salt evaporation pans that have essentially diked the entire southern end of the Dead Sea.

With its 14 spectral bands from the visible to the thermal infrared wavelength region, and its high spatial resolution of 15 to 90 meters (about 50 to 300 feet), ASTER images Earth to map and monitor the changing surface of our planet.

ASTER is one of five Earth-observing instruments launched December 18, 1999, on NASA's Terra satellite. The instrument was built by Japan's Ministry of Economy, Trade and Industry. A joint U.S./Japan science team is responsible for validation and calibration of the instrument and the data products.

The broad spectral coverage and high spectral resolution of ASTER provides scientists in numerous disciplines with critical information for surface mapping, and monitoring of dynamic conditions and temporal change. Example applications are: monitoring glacial advances and retreats; monitoring potentially active volcanoes; identifying crop stress; determining cloud morphology and physical properties; wetlands evaluation; thermal pollution monitoring; coral reef degradation; surface temperature mapping of soils and geology; and measuring surface heat balance.

The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission Directorate.

Size: 18.5 by 48.1 kilometers (11.5 by 29.8 miles) Location: 31.4 degrees North latitude, 35.4 degrees East longitude Orientation: North at top Image Data: ASTER bands 3, 2, and 1 Original Data Resolution: 15 meters (49.2 feet) Dates Acquired: May 3, 2005

2006-01-01

339

Caribbean Sea Level Network  

NASA Astrophysics Data System (ADS)

Over the past 500 years almost 100 tsunamis have been observed in the Caribbean and Western Atlantic, with at least 3510 people having lost their lives to this hazard since 1842. Furthermore, with the dramatic increase in population and infrastructure along the Caribbean coasts, today, millions of coastal residents, workers and visitors are vulnerable to tsunamis. The UNESCO IOC Intergovernmental Coordination Group for Tsunamis and other Coastal Hazards for the Caribbean and Adjacent Regions (CARIBE EWS) was established in 2005 to coordinate and advance the regional tsunami warning system. The CARIBE EWS focuses on four areas/working groups: (1) Monitoring and Warning, (2) Hazard and Risk Assessment, (3) Communication and (4) Education, Preparedness and Readiness. The sea level monitoring component is under Working Group 1. Although in the current system, it's the seismic data and information that generate the initial tsunami bulletins, it is the data from deep ocean buoys (DARTS) and the coastal sea level gauges that are critical for the actual detection and forecasting of tsunamis impact. Despite multiple efforts and investments in the installation of sea level stations in the region, in 2004 there were only a handful of sea level stations operational in the region (Puerto Rico, US Virgin Islands, Bermuda, Bahamas). Over the past 5 years there has been a steady increase in the number of stations operating in the Caribbean region. As of mid 2012 there were 7 DARTS and 37 coastal gauges with additional ones being installed or funded. In order to reach the goal of 100 operational coastal sea level stations in the Caribbean, the CARIBE EWS recognizes also the importance of maintaining the current stations. For this, a trained workforce in the region for the installation, operation and data analysis and quality control is considered to be critical. Since 2008, three training courses have been offered to the sea level station operators and data analysts. Other requirements and factors have been considered for the sustainability of the stations. The sea level stations have to potentially sustain very aggressive conditions of not only tsunamis, but on a more regular basis, hurricanes. Given the requirement that the data be available in near real time, for tsunami and other coastal hazard application, robust communication systems are also essential. For the local operator, the ability to be able to visualize the data is critical and tools like the IOC Sea level Monitoring Facility and the Tide Tool program are very useful. It has also been emphasized the need for these stations to serve multiple purposes. For climate and other research applications the data need to be archived, QC'd and analyzed. Increasing the user base for the sea level data has also been seen as an important goal to gain the local buy in; local weather and meteorological offices are considered as key stakeholders but for whom applications still need to be developed. The CARIBE EWS continues to look forward to working with other IOC partners including the Global Sea Level Observing System (GLOSS) and Sub-Commission for the Caribbean and Adjacent Regions (IOCARIBE)/GOOS, as well as with local, national and global sea level station operators and agencies for the development of a sustainable sea level network.

von Hillebrandt-Andrade, C.; Crespo Jones, H.

2012-12-01

340

Mountains in the Sea  

NSDL National Science Digital Library

In this 6-7 day investigation, learners begin with an introduction to seamounts that are present in the Gulf of Alaska. They learn how seamounts were formed and look at a bathymetric map of a seamount. In Activity 3A, learners explore sea floor mapping techniques as they participate in an activity to create a map of a sea feature they have molded out of clay. In Activity 3B, learners watch a short animated presentation, "Who cares about Sea Floor Mapping?" and create a model of a seamount found in Alaska. They use pre-sonar techniques to collect data and create a graph of their seamount using Excel. This detailed lesson plan includes learner hand-outs, evaluation questions, curricular connections, and tips.

Alaska Sea Grant

2011-01-01

341

SeaWeb  

NSDL National Science Digital Library

SeaWeb is a nonprofit organization aimed at raising awareness of the ocean and marine life that play "a critical role in our everyday life and in the future of our planet." SeaWeb employs a team of professionals from biology, exploration, and various communication disciplines. The current campaigns include an effort to protect the declining Caspian Sea Sturgeon ("the source of most of the world's caviar"), an attempt to reduce overfishing of swordfish, and a report about the changes occurring in the world's oceans. This Web site is a robust source of information about many threats that are facing marine ecosystems, and an attempt to reduce the dangers by educating the public about the impacts of their behavior.

342

Geology of Barents Sea  

SciTech Connect

The Barents Sea is situated on the continental shelf between Norway, the Spitsbergen Islands, and Novaya Zemlya. The main structural framework of the area was formed during the Caledonian and Hercynian orogenies, whereas the western parts were reactivated by the Kimmerian and Alpine orogenies. Because of the complex opening of the Greenland Norwegian Sea, important tertiary reactivation of Mesozoic normal faults occurred along southwest-northeast-trending systems of wrench faults. Owing to substantial erosion in the late Tertiary, the subsidence history and thermal development are more difficult to unravel in this area than in other places along the Norwegian Shelf. The erosion products were deposited in a huge sedimentary wedge extending onto the oceanic crust. The hydrocarbon discoveries in the Troms area in the southern part of the Barents Sea are encouraging for further exploration. However, the petroleum potential for large areas is not well known at this stage.

Riis, F.; Vollset, J.

1984-09-01

343

Microdistribution of Faunal Assemblages at Deep-Sea Hydrothermal Vents in the Southern Ocean  

PubMed Central

Chemosynthetic primary production by microbes supports abundant faunal assemblages at deep-sea hydrothermal vents, with zonation of invertebrate species typically occurring along physico-chemical gradients. Recently discovered vent fields on the East Scotia Ridge (ESR) in the Southern Ocean represent a new province of vent biogeography, but the spatial dynamics of their distinct fauna have yet to be elucidated. This study determines patterns of faunal zonation, species associations, and relationships between faunal microdistribution and hydrothermal activity in a vent field at a depth of 2,400 m on the ESR. Remotely operated vehicle (ROV) dives obtained high-definition imagery of three chimney structures with varying levels of hydrothermal activity, and a mosaic image of >250 m2 of seafloor co-registered with temperature measurements. Analysis of faunal microdistribution within the mosaiced seafloor reveals a consistent pattern of faunal zonation with increasing distance from vent sources and peak temperatures. Assemblages closest to vent sources are visibly dominated by a new species of anomuran crab, Kiwa n. sp. (abundance >700 individuals m?2), followed by a peltospiroid gastropod (>1,500 individuals m?2), eolepadid barnacle (>1,500 individuals m?2), and carnivorous actinostolid anemone (>30 individuals m?2). Peripheral fauna are not dominated by a single taxon, but include predatory and scavenger taxa such as stichasterid seastars, pycnogonids and octopus. Variation in faunal microdistribution on chimneys with differing levels of activity suggests a possible successional sequence for vent fauna in this new biogeographic province. An increase in ?34S values of primary consumers with distance from vent sources, and variation in their ?13C values also indicate possible zonation of nutritional modes of the vent fauna. By using ROV videography to obtain a high-resolution representation of a vent environment over a greater extent than previous studies, these results provide a baseline for determining temporal change and investigations of processes structuring faunal assemblages at Southern Ocean vents. PMID:23144754

Marsh, Leigh; Copley, Jonathan T.; Huvenne, Veerle A. I.; Linse, Katrin; Reid, William D. K.; Rogers, Alex D.; Sweeting, Christopher J.; Tyler, Paul A.

2012-01-01

344

SEAS Safety Program SEAS SAFETY PROGRAM 2013-2014  

E-print Network

Support · Linn Eichler & Christie Gilliland Safety Inspection Support · Lab Safety Officers (~ 40 safety Provided to SEAS Research Labs · Annual Lab Safety Inspections · Report and Feedback from/after Lab to your lab group · Report incidents to SEAS safety committee · Attend annual lab inspection (SEAS + EHSEM

345

RADIOCARBON RESERVOIR AGES IN THE MEDITERRANEAN SEA AND BLACK SEA  

Microsoft Academic Search

We measured apparent marine radiocarbon ages for the Mediterranean Sea, Black Sea, and Red Sea by accel- erator mass spectrometry radiocarbon analyses of 26 modern, pre-bomb mollusk shells collected living between AD 1837 and 1950. The marine reservoir (R(t)) ages were estimated at some 390 ± 85 yr BP, 415 ± 90 yr BP and 440 ± 40 yr BP,

Nadine Tisnerat; Franck Bassinot

346

Spectacled Eiders Wintering in Northern Bering Sea  

USGS Multimedia Gallery

Large flock of Spectacled Eiders aggregated in a sea ice lead and on sea ice in the northern Bering Sea, south of St. Lawrence Island, Alaska.  Aerial photograph from a helicopter deployed from the USCG Cutter Polar Sea....

347

National Sea Grant Library  

NSDL National Science Digital Library

This is the website for the only facility housing a complete collection of Sea Grant funded work. An archive and lending library for reprints, books, reports, maps, newsletters, handbooks, videos, CD-roms and computer programs regarding: oceanography; marine education; aquaculture; fisheries; limnology; coastal zone management; marine recreation and law. Lends documents worldwide, aiding scientists, teachers, students, fishermen and others in research and study. Bibliographic database is searchable from the website, where users may obtain citations, abstracts and access to over 20,000 downloadable texts of Sea Grant publications.

348

Lighting Up the Sea  

NSDL National Science Digital Library

This National Geographic lesson plan explores bioluminescent organisms in the sea. In this activity, students explore the benefits of bioluminescence by conducting a simulation and viewing pictures of bioluminescent marine animals on the Web. The conclusion of the activity entails students pretending to be deep-sea divers and writing journal entries about their impressions of a bioluminescent animal they have encountered. In addition to a detailed procedure, the lesson plan includes suggestions for assessment, ideas for extending the lesson, and links to related websites.

Xpeditions, National G.

349

Transdermal Delivery of Scopolamine by Natural Submicron Injectors: In-Vivo Study in Pig  

PubMed Central

Transdermal drug delivery has made a notable contribution to medical practice, but has yet to fully achieve its potential as an alternative to oral delivery and hypodermic injections. While transdermal delivery systems would appear to provide an attractive solution for local and systemic drug delivery, only a limited number of drugs can be delivered through the outer layer of the skin. The most difficult to deliver in this way are hydrophilic drugs. The aquatic phylum Cnidaria, which includes sea anemones, corals, jellyfish and hydra, is one of the most ancient multicellular phyla that possess stinging cells containing organelles (cnidocysts), comprising a sophisticated injection system. The apparatus is folded within collagenous microcapsules and upon activation injects a thin tubule that immediately penetrates the prey and delivers its contents. Here we show that this natural microscopic injection system can be adapted for systemic transdermal drug delivery once it is isolated from the cells and uploaded with the drug. Using a topically applied gel containing isolated natural sea anemone injectors and the muscarinic receptor antagonist scopolamine, we found that the formulated injectors could penetrate porcine skin and immediately deliver this hydrophilic drug. An in-vivo study in pigs demonstrated, for the first time, rapid systemic delivery of scopolamine, with Tmax of 30 minutes and Cmax 5 times higher than in controls treated topically with a scopolamine-containing gel without cnidocysts. The ability of the formulated natural injection system to penetrate a barrier as thick as the skin and systemically deliver an exogenous compound presents an intriguing and attractive alternative for hydrophilic transdermal drug delivery. PMID:22363770

Shaoul, Esther; Ayalon, Ari; Tal, Yossi; Lotan, Tamar

2012-01-01

350

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles  

NASA Astrophysics Data System (ADS)

Endobiotic bacteria colonize the tentacles of cnidaria. This paper provides first insight into the bacterial spectrum and its potential of pathogenic activities inside four cnidarian species. Sample material originating from Scottish waters comprises the jellyfish species Cyanea capillata and C. lamarckii, hydrozoa Tubularia indivisa and sea anemone Sagartia elegans. Mixed cultures of endobiotic bacteria, pure cultures selected on basis of haemolysis, but also lyophilized samples were prepared from tentacles and used for DGGE-profiling with subsequent phylogenetic analysis of 16S rDNA fragments. Bacteria were detected in each of the cnidarian species tested. Twenty-one bacterial species including four groups of closely related organisms were found in culture material. The species within these groups could not be differentiated from each other (one group of Pseudoalteromonas spp., two groups of Shewanella spp., one group of Vibrio spp.). Each of the hosts exhibits a specific endobacterial spectrum. Solely Cyanea lamarckii harboured Moritella viscosa. Only in Cyanea capillata, members of the Shewanella group #2 and the species Pseudoalteromonas arctica, Shewanella violacea, Sulfitobacter pontiacus and Arcobacter butzleri were detected. Hydrozoa Tubularia indivisa provided an amazingly wide spectrum of nine bacterial species. Exclusively, in the sea anemone Sagartia elegans, the bacterial species P. aliena was found. Overall eleven bacterial species detected were described recently as novel species. Four 16S rDNA fragments generated from lyophilized material displayed extremely low relationship to their next neighbours. These organisms are regarded as members of the endobiotic “terra incognita”. Since the origin of cnidarian toxins is unclear, the possible pathogenic activity of endobiotic bacteria has to be taken into account. Literature data show that their next neighbours display an interesting diversity of haemolytic, septicaemic and necrotic actions including the production of cytotoxins, tetrodotoxin and R-toxin. Findings of haemolysis tests support the literature data. The potential producers are Endozoicimonas elysicola, Moritella viscosa, Photobacterium profundum, P. aliena, P. tetraodonis, Shewanella waksmanii, Vibrio splendidus, V. aestuarius, Arcobacter butzleri.

Schuett, Christian; Doepke, Hilke

2010-09-01

351

Jellyfish as prey: frequency of predation and selective foraging of Boops boops (Vertebrata, Actinopterygii) on the mauve stinger Pelagia noctiluca (Cnidaria, Scyphozoa).  

PubMed

In recent years, jellyfish blooms have attracted considerable scientific interest for their potential impacts on human activities and ecosystem functioning, with much attention paid to jellyfish as predators and to gelatinous biomass as a carbon sink. Other than qualitative data and observations, few studies have quantified direct predation of fish on jellyfish to clarify whether they may represent a seasonally abundant food source. Here we estimate predation frequency by the commercially valuable Mediterranean bogue, Boops boops on the mauve stinger jellyfish, Pelagia noctiluca, in the Strait of Messina (NE Sicily). A total of 1054 jellyfish were sampled throughout one year to quantify predation by B. boops from bite marks on partially eaten jellyfish and energy density of the jellyfish. Predation by B. boops in summer was almost twice that in winter, and they selectively fed according to medusa gender and body part. Calorimetric analysis and biochemical composition showed that female jellyfish gonads had significantly higher energy content than male gonads due to more lipids and that gonads had six-fold higher energy content than the somatic tissues due to higher lipid and protein concentrations. Energetically, jellyfish gonads represent a highly rewarding food source, largely available to B. boops throughout spring and summer. During the remainder of the year, when gonads were not very evident, fish predation switched towards less-selective foraging on the somatic gelatinous biomass. P. noctiluca, the most abundant jellyfish species in the Mediterranean Sea and a key planktonic predator, may represent not only a nuisance for human leisure activities and a source of mortality for fish eggs and larvae, but also an important resource for fish species of commercial value, such as B. boops. PMID:24727977

Milisenda, Giacomo; Rosa, Sara; Fuentes, Veronica L; Boero, Ferdinando; Guglielmo, Letterio; Purcell, Jennifer E; Piraino, Stefano

2014-01-01

352

FHL Organism Collection Form Name of Researcher or Student: ___________________________________________  

E-print Network

. NUMBER Sponges Cephalopods Anemones Nudibranchs Polychaetes Starfish Cancer crabs Urchins Other crabs Sea cucumbers Shrimp Ascidians Snails Flat fish Chitons Other fish Bivalves MULTIPLE COLLECTIONS (OF A FEW

Carrington, Emily

353

FHL Organism Collection Form Name of Researcher or Student: ___________________________________________  

E-print Network

ORGANISM APROX. NUMBER Sponges Cephalopods Anemones Nudibranchs Polychaetes Starfish Cancer crabs Urchins Other crabs Sea cucumbers Shrimp Ascidians Snails Flat fish Chitons Other fish Bivalves MULTIPLE

Carrington, Emily

354

The relationship of the abundance and distribution of the sea anemone Bunodosoma cavernata bosc to variations in the physical environment in the rocky intertidal habitats of the north Texas coast  

E-print Network

conspicuous species, occurring in large numbers on hard and soft substrates. Porcelain crabs (Petrolisthes armatus) and mall d ah (~githr harrisii) also ha da t. Bar 1 d the t-f 1 g mphlp d ~thrum 1 1 la m separately form thick layers on wave-swept slabs.... harrisii and P. armatus are common under most larger rocks while C. vittatus and M. mercenaria occur infrequently around the bases of the granite. S 11 st di11s (thai ~ht ) ho f d gth barnacles. In the outer third of the site all permanent surfaces...

Anderson, Elise Marie

1988-01-01

355

Sea Level : Frequently Asked Questions and Answers  

NSDL National Science Digital Library

Visitors can find answers to frequently asked questions regarding sea level and sea level changes. Topics addressed include how mean sea level is defined, how much sea level would rise if all the worlds ice were to melt, differences in sea level between oceans and at different latitudes, the meaning of altitude above sea level, and others.

2007-12-12

356

Chaotic dynamics of sea clutter  

Microsoft Academic Search

rules for the underlying sea clutter dynamics, in contrast to the stochastic approach where sea clutter is viewed as a random process with a large number of degrees of freedom. In this paper, we demonstrate, convincingly for the first time, the chaotic dynamics of sea clutter. We say so on the basis of results obtained using radar data collected from

Simon Haykin; Sadasivan Puthusserypady

1997-01-01

357

3, 637669, 2006 Mediterranean Sea  

E-print Network

OSD 3, 637­669, 2006 Central Mediterranean Sea forecast S. Natale et al. Title Page Abstract Mediterranean Sea forecast: effects of high-resolution atmospheric forcings S. Natale1 , R. Sorgente2 , S­669, 2006 Central Mediterranean Sea forecast S. Natale et al. Title Page Abstract Introduction Conclusions

Paris-Sud XI, Université de

358

SEA LAMPREY SPAWNING: MICHIGAN STREAMS  

E-print Network

SEA LAMPREY SPAWNING: MICHIGAN STREAMS OF LAKE SUPERIOR Marine Bioiogical Laboratory MAY 2 3 1952 Service, Albert M. Day, Director SEA LAMPREY SPAWNING s MICHIGAN STREAMS OF LAKE SUPERIOR by Howard A decades since the capture of the first specimen in Lake Erie in 1921, the sea lamprey ( Petromyzon marinus

359

Tides & Currents: Sea Level Trends  

NSDL National Science Digital Library

The Center for Operational Oceanographic Products and Services has been measuring sea level for over 150 years. This resource illustrates regional trends in sea level, with arrows representing the direction and magnitude of change including national and global stations. Impacts on changing sea levels in relation to atmospheric and oceanic processes as well as other Earth systems are explained and supported with educations resources.

2010-01-01

360

Sinking cities. [Rising sea level  

Microsoft Academic Search

Although the rapid subsidence of the last 50 years now appears slowed or even arrested in many cities, there has been almost no recovery in ground elevation, and many of the world's great cities remain vulnerable to flooding from the sea. The continuing rise in sea level challenges the engineering efforts designed to keep out the sea. Over the past

R. Dolan; H. G. Goodell

2009-01-01

361

National Sea Grant Educators Network  

NSDL National Science Digital Library

Compilation of Sea Grant marine education resources. Site includes the latest news, a pdf file discussing Sea Grant education initiatives, links to all Sea Grant Education websites, several teaching and learning resources, and several interactive classroom activities. An excellent site to begin preparations for a marine science or oceanography course.

362

SeaWeb  

NSDL National Science Digital Library

A multimedia public education project designed to raise awareness of the world ocean and the life within it. Find articles on the latest ocean issues, links to resources and audio clips of the radio show Ocean Report. Also features information on SeaWeb programs, such as aquaculture initiatives for both fish and their eggs (caviar), and publications.

363

Solar Sea Power  

ERIC Educational Resources Information Center

In their preoccupation with highly complex new energy systems, scientists and statesmen may be overlooking the possibilities of Ocean Thermal Energy Conversion (OTEC). That is the view of a Carnegie-Mellon University physicist who is in the forefront of solar sea power investigation. (Author/BT)

Zener, Clarence

1976-01-01

364

Ships to the Sea.  

ERIC Educational Resources Information Center

This lesson contains materials for the U.S. Navy Museum's "Ships to the Sea" program. The program is appropriate for students in grades 2-4 and was designed in accordance with local and national social studies standards. The materials introduce students to the world of ship technology and naval terminology. The lesson is presented in five…

Department of the Navy, Washington, DC.

365

Classroom of the Sea  

NSDL National Science Digital Library

Although most students do not have the opportunity to conduct in situ research projects until college, the Classroom of the Sea program at the American School for the Deaf (ASD) provides an unusual opportunity for students to work directly with scientists

Monte, Denise; Hupper, Mary L.; Scheifele, Peter

2000-03-01

366

Sea Lion Skeleton - Backbone  

NSDL National Science Digital Library

Sea lions are vertebrates with both backbones and ribs. The backbone is a gliding joint, allowing the animal to be flexible, while the ribs main function is to protect it's inner organs. The short tail helps to balance the animal while walking on land.

Ketan Patel (California State University, Fullerton; Student, Biological Sciences)

2007-07-27

367

Sea Turtle Skeleton - Skull  

NSDL National Science Digital Library

The carnivorous sea turtle's skull has two eye sockets for good vision on each side along with nostrils to detect prey. It does not have teeth, but has sharp edges on its jaw to rip and tear food. The skull protects the brain from damage and injury.

Ketan Patel (California State University, Fullerton;Student, Biological Sciences)

2007-07-26

368

Sea Turtle Skeleton - Nostrils  

NSDL National Science Digital Library

The carnivorous sea turtle's skull has two eye sockets for good vision on each side along with nostrils to detect prey. It does not have teeth, but has sharp edges on its jaw to rip and tear food. The skull protects the brain from damage and injury.

Ketan Patel (California State University, Fullerton;Student, Biological Sciences)

2007-07-26

369

Classroom of the Sea.  

ERIC Educational Resources Information Center

Introduces the Sea Program in which participant students were deaf and collaborated with a bioacoustician. Studies the underwater noise levels of the Gulf of Maine and the possible impacts on marine life. Explains implementing this project in the science curriculum. (YDS)

Hupper, Mary Laporta; Monte, Denise; Scheifele, Peter

2000-01-01

370

Sea Turtles Coloring Book  

NSDL National Science Digital Library

Using excellent line drawings, this coloring book provides great background information about sea turtles in both English and Spanish. Species profiles, biology and life cycle, ecology, distribution, uses and threats, like fishing and habitat destruction, are covered. Measures for protection and conservation are introduced.

371

Redlands Institute: Salton Sea  

NSDL National Science Digital Library

The Salton Sea Database Program (SSDP) at the University of Redlands, Redlands Institute (RI) was a project administered by the U.S. Environmental Protection Agency (EPA), Center for Special Programs. The purpose behind the SSDP was to bring a wide range of data management and analysis tools and professionals "to support multi-disciplinary and coordinated decision-making across all the professional and scientific teams and stakeholders involved in the restoration of California's largest inland body of water, the Salton Sea." Part of their outreach work includes this fine website, which includes sections titled "Ecological Issues", "Data & Research", and "Exploring the Area". First-time visitors may want to start by clicking on the "Ecological Issues" area. Here they can learn about the science of the area, the contemporary issues facing the survival of the Salton Sea, and some of the proposed solutions to restore the Sea. Journalists and scientists will appreciate the "Data & Research" area, as it features digital maps of the area, GIS data, public policy documents, and an image database. The casual traveler will enjoy the "Exploring the Area" section, and here they will find information about current weather conditions, fishing reports, and California State park materials.

372

Black Sea Battle  

NSDL National Science Digital Library

Story of the invasion of jellyfish into the Black Sea and the resulting affects on the native fish population. An excellent introduction into introduced species and their effects on an ecosystem. Site features an abundance of information on alien species and the delicacy that goes into eradicating them. Also links to additional non-traditional science-related news events.

373

Solomon's Sea and [Pi  

ERIC Educational Resources Information Center

This paper is a whimsical survey of the various explanations which might account for the biblical passage in I Kings 7:23 that describes a round object--a bronze basin called Solomon's Sea--as having diameter ten cubits and circumference thirty cubits. Can the biblical pi be any number other than 3? We offer seven different perspectives on this…

Simoson, Andrew J.

2009-01-01

374

Rising Sea Levels  

NSDL National Science Digital Library

In the past century, as the climate has warmed, sea level rise has accelerated. Scientists predict it will only increase, and they're studying changes in the ocean and land to better understand how and why the water is rising. "Changing Planet" is produced in partnership with the National Science Foundation.

Learn, Nbc

2010-10-07

375

Sea Level Viewer  

NSDL National Science Digital Library

This interactive program focuses on the role of sea level in climate change. Sections include an overview and a list of relevant NASA satellite missions and their objectives. A third section, entitled Global View, covers the following 5 topics: Latest View, Large El Niño, Hurricane Katrina, Indian Ocean Tsunami, and La Niña.

2013-07-09

376

Egyptian Sea Cave  

ERIC Educational Resources Information Center

This brief article describes an archaeological expedition to the Red Sea coast area of Egypt in 2004. Kathryn Bard, an associate professor of archaeology at Boston University, along with her team, discovered the well-preserved cedar timbers of an ancient Egyptian seafaring vessel near the entrance to a large man-made cave. Limestone tablets with…

Journal of College Science Teaching, 2005

2005-01-01

377

A closing Ligurian Sea?  

Microsoft Academic Search

Two earthquakes occurred in the Ligurian Sea in December 1989 and April 1990. Both were widely felt along the French and Italian Rivieras, thus reminding us of the seismic risk in this region. The significant increase in the number of seismic stations in the area facilitated the study of these two shocks and their related aftershocks. Using different techniques (absolute

N. Bethoux; J. Fréchet; F. Guyoton; F. Thouvenot; M. Cattaneo; C. Eva; M. Nicolas; M. Granet

1992-01-01

378

Steller Sea Lion  

NSDL National Science Digital Library

A comprehensive site featuring the threatened Steller Sea Lion. Site includes information on its critical habitat, protection measures, distribution information, and much more. Explore the Literature, Presentations, and Images section for an abundance of information from conferences, workshops, and research. Site features a wealth of photographs and video, as well as contact information for the program.

2011-01-10

379

The Sea Around Us  

Microsoft Academic Search

Published in 1951, The Sea Around Us is one of the most remarkably successful books ever written about the natural world. Rachel Carson's rare ability to combine scientific insight with moving, poetic prose catapulted her book to first place on The New York Times best-seller list, where it enjoyed wide attention for thirty-one consecutive weeks. It remained on the list

Rachel L. Carson

1991-01-01

380

SeaWiFS analysis of the Japan and East China Sea air/sea environment.  

E-print Network

??Using visible wavelength radiance data obtained from the spaceborne Sea-viewing Wide Fieldof- view Sensor (SeaWiFS), during the Aerosol Characterization Experiment-Asia (ACE-Asia), an analysis of the… (more)

Rocha, James D.

2001-01-01

381

The Weddell Sea  

NASA Technical Reports Server (NTRS)

Several large, irregularly shaped icebergs are floating in the Weddell Sea, east of the Antarctic Peninsula, in this true-color MODIS image from February 17, 2002. The location of several of the bergs has changed little over the last three months. Compared to an image acquired on November 13, 2001, the berg at the upper right of the image has spun around, but is still hanging around in the same general location. Similar slow-movers can be seen just to the east of the Larsen Ice Shelf, which hugs the eastern coast of the Peninsula. The northernmost of those two bergs is designated A38b; the southernmost one is A38a. These bergs were once part of an iceberg greater than 2,700 square miles that broke off the Ronne Ice Shelf (to the south) back in 1998. While the waters of the Weddell Sea in the area ought to be deep enough to float those bergs, it is possible that they have run aground on a topographic high, or ridge, in the sea floor. However, little is known about the underwater topography of that region, and it is also possible that the bergs are simply so massive that they resist being moved by surface wind or ocean currents. While four years might seem like a long time for an iceberg to hang around, these are certainly no record holders. A berg that broke off the Ross Ice Shelf (on the other side of Antarctica) drifted north and went aground south of Australia. That berg calved in 1987, and hasn't really moved in ten years. While the big bergs have not moved much in the span of time between these images, there is a big difference in the amount of sea ice present in the two images. In general, the rounder chunks of ice are more likely to be seasonal sea ice that forms from the freezing of sea water, while the larger, jagged-edged pieces of ice are more likely to be bergs that broke off an ice shelf at the margin of the continent. It's the height of summer in Antarctica in the February image, and much of the sea ice has melted or drifted away, leaving a relatively large expanse of clear ocean. Credit:

2002-01-01

382

Sea floor magnetic observatory  

NASA Astrophysics Data System (ADS)

The electromagnetic precursors of seismic hazards are widely accepted as strong evidence of the approaching earthquake or volcano eruption. The monitoring of these precursors are of main interest in densely populated areas, what creates serious problems to extract them at the strong industrial noise background. An interesting possibility to improve signal-to-noise ratio gives the installation of the observation points in the shelf zones near the possible earthquake places, what is fairly possible in most seismically active areas in Europe, e. g. in Greece and Italy. The serious restriction for this is the cost of the underwater instrumentation. To realize such experiments it requires the unification of efforts of several countries (e. g., GEOSTAR) or of the funds of some great companies (e. g., SIO magnetotelluric instrument). The progress in electronic components development as well as the appearance of inexpensive watertight glass spheres made it possible to decrease drastically the price of recently developed sea floor magnetic stations. The autonomous vector magnetometer LEMI-301 for sea bed application is described in the report. It is produced on the base of three-component flux-gate sensor. Non-magnetic housing and minimal magnetism of electronic components enable the instrument to be implemented as a monoblock construction where the electronic unit is placed close to the sensor. Automatic circuit provides convenient compensation of the initial field offset and readings of full value (6 digits) of the measured field. Timing by internal clock provides high accuracy synchronization of data. The internal flash memory assures long-term autonomous data storage. The system also has two-axes tilt measurement system. The methodological questions of magnetometer operation at sea bed were studied in order to avoid two types of errors appearing at such experimental cases. First is sea waving influence and second one magnetometer orientation at its random positioning on the sea floor in order to get experimental data in geomagnetic coordinates frames. The analysis executed showed that first error source can not be avoided at shallow water experiments but can be easily taken into account. The special methodology and the developed software allowed to solve the second problem. It was shown that it is possible to reduce the magnetometer data collected in randomly oriented coordinate system at arbitrary position on the sea floor to the data in the frame system connected with geomagnetic coordinates. The parameters of LEMI-302 sea bed magnetometer are discussed and the experimental results of its application are presented. The research work in Ukraine was partly supported by INTAS grant 99-1102.

Korepanov, V.; Prystai, A.; Vallianatos, F.; Makris, J.

2003-04-01

383

Effects of five oleanolic acid triterpenoid saponins from the rhizome of Anemone raddeana on stimulus-induced superoxide generation, phosphorylation of proteins and translocation of cytosolic compounds to cell membrane in human neutrophils.  

PubMed

Five oleanolic acid triterpenoid saponins (OTS-1, 2, 3, 4 and 5) were isolated from the rhizome of Anemone raddeana. The effect of these triterpenoid saponins on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of ferricytochrome c using a dual-beam spectrophotometer. The phosphorylation of neutrophil proteins, and translocation of p67(phox), p47(phox) and Rac to plasma membrane were investigated using specific monoclonal antibodies. The five oleanolic acid triterpenoid saponins used in this experiment suppressed N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in a concentration-dependent manner. OTS-1, 2 and 4 suppressed phorbol 12-myristate 13-acetate (PMA)- and arachidonic acid (AA)-induced superoxide generation in a concentration-dependent manner, but OTS-3 and 5 showed no effect. fMLP- and PMA-induced tyrosyl or serine/threonine phosphorylation, and fMLP-, PMA- and AA-induced translocation of p67(phox), p47(phox) and Rac to plasma membrane were in parallel with the suppression of the stimulus-induced superoxide generation. PMID:22178681

Wei, Shihu; He, Wenfei; Lu, Jincai; Wang, Zhonghuan; Yamashita, Koichi; Yokoyama, Masanori; Kodama, Hiroyuki

2012-03-01

384

Northern Sand Sea  

NASA Technical Reports Server (NTRS)

[figure removed for brevity, see original site]

Our topic for the weeks of April 4 and April 11 is dunes on Mars. We will look at the north polar sand sea and at isolated dune fields at lower latitudes. Sand seas on Earth are often called 'ergs,' an Arabic name for dune field. A sand sea differs from a dune field in two ways: 1) a sand sea has a large regional extent, and 2) the individual dunes are large in size and complex in form.

This VIS image was taken at 82 degrees North latitude during Northern spring. The image is completely dominated by dunes. In sand seas, it is very common for a single type of dune to occur, and for a single predominate wind to control the alignment of the dunes.

Image information: VIS instrument. Latitude 82.2, Longitude 152.5 East (207.5 West). 19 meter/pixel resolution.

Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time.

NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the California Institute of Technology in Pasadena.

2005-01-01

385

Sea ice radiative forcing, sea ice area, and climate sensitivity  

NASA Astrophysics Data System (ADS)

Changes in sea ice cover affect climate sensitivity by modifying albedo and surface heat flux exchange, which in turn affect the absorbed solar radiation at the surface as well as cloud cover, atmospheric water content and poleward atmospheric heat transport. Here, we use a configuration of the Community Earth System Model 1.0.4 with a slab ocean model and a thermodynamic-dynamic sea ice model to investigate the overall net effect of feedbacks associated with the sea ice loss. We analyze the strength of the overall sea ice feedback in terms of two factors: the sensitivity of sea ice area to changes in temperature, and the sensitivity of sea ice radiative forcing to changes in sea ice area. In this model configuration, sea ice area decreases by ~3 × 1012 m2 per K of global warming, while the effective global radiative forcing per square meter of sea ice loss is ~0.1 × 10-12 W m-2. The product of these two terms (~0.3 W m-2 K-1) approximately equals the difference in climate feedback parameter found in simulations with sea ice response (1.05 W m-2 K-1) and simulations without sea ice response (1.31 W m-2 K-1 or 1.35 W m-2 K-1, depending on the method used to disable changes in sea ice cover). Thus, we find that in our model simulations, sea ice response accounts for about 20% to 22% of the climate sensitivity to an imposed change in radiative forcing. In our model, the additional radiative forcing resulting from a loss of all sea ice in the 'pre-industrial' state is comparable to but somewhat less than the radiative forcing from a doubling of atmospheric CO2 content.

Caldeira, Ken; Cvijanovic, Ivana

2014-05-01

386

The North Sea - A shelf sea in the Anthropocene  

NASA Astrophysics Data System (ADS)

Global and regional change clearly affects the structure and functioning of ecosystems in shelf seas. However, complex interactions within the shelf seas hinder the identification and unambiguous attribution of observed changes to drivers. These include variability in the climate system, in ocean dynamics, in biogeochemistry, and in shelf sea resource exploitation in the widest sense by societies. Observational time series are commonly too short, and resolution, integration time, and complexity of models are often insufficient to unravel natural variability from anthropogenic perturbation. The North Sea is a shelf sea of the North Atlantic and is impacted by virtually all global and regional developments. Natural variability (from interannual to multidecadal time scales) as response to forcing in the North Atlantic is overlain by global trends (sea level, temperature, acidification) and alternating phases of direct human impacts and attempts to remedy those. Human intervention started some 1000 years ago (diking and associated loss of wetlands), expanded to near-coastal parts in the industrial revolution of the mid-19th century (river management, waste disposal in rivers), and greatly accelerated in the mid-1950s (eutrophication, pollution, fisheries). The North Sea is now a heavily regulated shelf sea, yet societal goals (good environmental status versus increased uses), demands for benefits and policies diverge increasingly. Likely, the southern North Sea will be re-zoned as riparian countries dedicate increasing sea space for offshore wind energy generation - with uncertain consequences for the system's environmental status. We review available observational and model data (predominantly from the southeastern North Sea region) to identify and describe effects of natural variability, of secular changes, and of human impacts on the North Sea ecosystem, and outline developments in the next decades in response to environmental legislation, and in response to increased use of shelf sea space.

Emeis, Kay-Christian; van Beusekom, Justus; Callies, Ulrich; Ebinghaus, Ralf; Kannen, Andreas; Kraus, Gerd; Kröncke, Ingrid; Lenhart, Hermann; Lorkowski, Ina; Matthias, Volker; Möllmann, Christian; Pätsch, Johannes; Scharfe, Mirco; Thomas, Helmuth; Weisse, Ralf; Zorita, Eduardo

2015-01-01

387

Early and late response of Nematostella vectensis transcriptome to heavy metals.  

PubMed

Environmental contamination from heavy metals poses a global concern for the marine environment, as heavy metals are passed up the food chain and persist in the environment long after the pollution source is contained. Cnidarians play an important role in shaping marine ecosystems, but environmental pollution profoundly affects their vitality. Among the cnidarians, the sea anemone Nematostella vectensis is an advantageous model for addressing questions in molecular ecology and toxicology as it tolerates extreme environments and its genome has been published. Here, we employed a transcriptome-wide RNA-Seq approach to analyse N. vectensis molecular defence mechanisms against four heavy metals: Hg, Cu, Cd and Zn. Altogether, more than 4800 transcripts showed significant changes in gene expression. Hg had the greatest impact on up-regulating transcripts, followed by Cu, Zn and Cd. We identified, for the first time in Cnidaria, co-up-regulation of immediate-early transcription factors such as Egr1, AP1 and NF-?B. Time-course analysis of these genes revealed their early expression as rapidly as one hour after exposure to heavy metals, suggesting that they may complement or substitute for the roles of the metal-mediating Mtf1 transcription factor. We further characterized the regulation of a large array of stress-response gene families, including Hsp, ABC, CYP members and phytochelatin synthase, that may regulate synthesis of the metal-binding phytochelatins instead of the metallothioneins that are absent from Cnidaria genome. This study provides mechanistic insight into heavy metal toxicity in N. vectensis and sheds light on ancestral stress adaptations. PMID:25145541

Elran, Ron; Raam, Maayan; Kraus, Roey; Brekhman, Vera; Sher, Noa; Plaschkes, Inbar; Chalifa-Caspi, Vered; Lotan, Tamar

2014-10-01

388

Biodiversity of the Deep-Sea Benthic Fauna in the Sangihe-Talaud Region, Indonesia: Observations from the INDEX-SATAL 2010 Expedition  

NASA Astrophysics Data System (ADS)

The benthic ecosystems found in the deep-sea promontories of Sangihe Talaud region were explored, between June and August 2010, using the ROV Little Hercules aboard the NOAA ship Okeanos Explorer. The Sangihe-Talaud region is part of the Coral Triangle (CT) an area known for harboring the most biodiverse shallow-water coral reefs in the world. Notwithstanding the significant research efforts that have been undertaken to catalog and protect the biodiversity of the CT prior this expedition, virtually nothing was known about the life inhabiting the deep sea. The high-resolution imagery obtained from the 27 ROV dives revealed remarkably high abundances and diversity of animal species, many of which appear to be novel. On hard bottom substrates, cold-water corals were the dominant sessile macrofauna, in terms of biomass, followed by glass sponges (Hexactinellida) and sea lilies (Crinoidea). The coral taxa observed in this area represent six large orders of cnidarians: antipatharians (black corals), scleractinians (stony corals), zoanthideans (gold corals), alcyonaceans (octocorals), pennatulaceans (sea pens), and anthoathecates (hydrocorals). Most sessile species, independently of their size class or taxonomic affiliation, harbor a wide variety of associated fauna. Brittle stars (Ophiuroidea), squat lobsters (Galatheoidea), shrimp (Caridea), amphipods (Amphipoda), anemones (Actinaria), zanthideans, barnacles (Cirripedia), hydroids (Hydrozoa) and worms (Polychaeta) are the animal groups most commonly found forming these associations. In contrast, soft bottom habitats were dominated by stalked sponges, sea pens, sea cucumbers (Holothuroidea) and brittle stars. Other conspicuous fauna include fish, hermit crabs (Paguridae), urchins (Echinoidea) and octopuses (Cephalopoda). The abundance of habitats generated by the high number of geological and biological features and depth ranges present in the deep coral triangle (e.g., ridges, seamounts, island margins, plains, and rock types), and the complex history of tectonic dynamics of this region are among the likely causes for the high biodiversity found during this mission. Tectonic history and diverse habitats may also be factors that have played a similar role shaping the diversity of shallow water assemblages of the region. Such parallels between the biodiversity of deep and shallow waters will be presented.

Herrera, S.; Munro, C.; Nganro, N.; Tunnicliffe, V.; Wirasantosa, S.; Sibert, E.; Hammond, S. R.; Bors, E.; Butterfield, D.; Holden, J. F.; Baker, E. T.; Sherrin, J.; Makarim, S.; Troa, R.; Shank, T. M.

2010-12-01

389

The Disappearing Aral Sea  

NSDL National Science Digital Library

In just 30 years, the Aral Sea has lost more than 60 percent of its water. Barring change, it may disappear entirely by 2020. In this visualization, satellite images dating from 1973 to 2000 show how water diverted from this inland lake for agriculture has caused it to shrink considerably over a short period of time. The feature can be run as an animation or as a series of slides. A background essay and discussion questions are included.

390

Changing Sea Levels  

NASA Astrophysics Data System (ADS)

Flooding of coastal communities is one of the major causes of environmental disasters world-wide. This textbook explains how sea levels are affected by astronomical tides, weather effects, ocean circulation and climate trends. Based on courses taught by the author in the U.K. and the U.S., it is aimed at undergraduate students at all levels, with non-basic mathematics being confined to Appendices and a website http://publishing.cambridge.org/resources/0521532183/.

Pugh, David

2004-04-01

391

Dauphin Island Sea Lab  

NSDL National Science Digital Library

Dauphin Island Sea Lab is Alabama's marine education and research center. Lab also provides a public aquarium that focuses solely on the native eco-systems of the Mobile Bay estuary. Site provides information on graduate programs, undergraduate opportunities, faculty, facilities, and news and events. Explore the Education and Aquarium sections for teacher resources and information on workshops, student summer camps, and academic-year programs.

392

WINDandSEA  

NSDL National Science Digital Library

Site was built in response to the many reference questions that are posed to the library and is meant to make internet searching more efficient for those concerned with oceanic and atmospheric issues, and the general public. Presently WINDandSEA has over 1,000 links to science and policy sites organized by topic and alphabetically within topic. All sites have been reviewed and annotated by NOAA staff.

393

Sea Floor Spreading  

NSDL National Science Digital Library

Sea floor spreading is demonstrated using a model consisting of two classroom desks and an 8-foot strip of paper. Changes in polarity are indicated using a felt marker. The investigation supports material presented in chapter 3, "What Heats the Earth's Interior?" in the textbook Energy flow, part of the Global System Science (GSS), an interdisciplinary course for high school students that emphasizes how scientists from a wide variety of fields work together to understand significant problems of global impact.

394

Sea & Ships: Explore online  

NSDL National Science Digital Library

The National Maritime Museum (NMM) in England notes that its goal is "working to illustrate for everyone the importance of the sea, ships, time and the stars and their relationship with people." There is so much to explore in the "Sea and Ships" portion of the NMM website, but a great way to see everything it has to offer is by using the "Sea and Ships Directory" at the bottom of the homepage. It divides the material up by "Subjects", "People", "Collections", "Online Galleries", and "Games and Interactives". Visitors interested in lessons about the ocean that come in the form of games, quizzes and stories, should definitely check out the "Your Ocean" link from the "Games and Interactives". The "Your Waste" lesson gives visitors the opportunity to test their skills at "managing an oil spill clean-up operation", in the game "Oil Crisis!" Keeping waste to a minimum is what the quiz "Pollution Solutions" addresses, and is also on the "Your Waste" page. Other lessons include "Your Energy", "Your Stuff" and "Your Climate".

395

Bering Sea in Bloom  

NASA Technical Reports Server (NTRS)

The surface waters of the Bering Sea around the Pribilof Islands, off Alaska's west coast, exhibited a dark green color on May 15, 2002, in this SeaWiFS true-color image. The green color of the currents there suggests the presence of a large phytoplankton bloom. Careful inspection reveals some a reddish tinges of light reflected by this bloom, particularly in a long east to west band just south of the Pribilof Islands, and just north of the Aleutian Island chain (disappearing toward the lower righthand corner of this scene beneath the cloud bank). Some scientists speculate this could be another Phaeocystis bloom, similar to the bloom of this species that was observed in these waters roughly this time last year. Such blooms are typically accompanied by a strong, unpleasant odor in the immediate vicinity. The light brown color of the surface waters along the Alaskan shoreline are probably due to suspended sediments washed off from the land. Image courtesy the SeaWiFS Project, NASA GSFC, and ORBIMAGE

2002-01-01

396

Aral Sea Evaporation (WMS)  

NSDL National Science Digital Library

The Aral Sea is actually not a sea at all, but an immense fresh water lake. In the last thirty years, more than sixty percent of the lake has disappeared because much of the river flow feeding the lake was diverted to irrigate cotton fields and rice paddies. Concentrations of salts and minerals began to rise in the shrinking body of water, leading to staggering alterations in the lakes ecology and precipitous drops in the Arals fish population. Powerful winds that blow across this part of Asia routinely pick up and deposit the now exposed lake bed soil. This has contributed to a significant reduction in breathable air quality, and crop yields have been appreciably affected due to heavily salt laden particles falling on arable land. This series of Landsat images taken in 1973, 1987 and 2000 show the profound reduction in overall area at the north end of the Aral, and a commensurate increase in land area as the floor of the sea now lies exposed.

Joycelyn Thomson

2005-02-15

397

Kara Sea radioactivity assessment.  

PubMed

Investigations following five international expeditions to the Kara Sea have shown that no radiologically significant contamination has occurred outside of the dumping sites in Novaya Zemlya bays. Increased levels of radionuclides in sediment have only been observed in Abrosimov and Stepovoy Bays very close to dumped containers. Evaluations of radionuclide inventories in water and sediment of the open Kara Sea and Novaya Zemlya bays as well as soil from the shore of Abrosimov bay have shown that radionuclide contamination of the open Kara Sea is mainly due to global fallout, with smaller contributions from the Sellafield reprocessing plant, the Chernobyl accident run-off from the Ob and Yenisey rivers and local fallout. Computer modelling results have shown that maximum annual doses of approximately 1 mSv are expected for a hypothetical critical group subsisting on fish caught in the Novaya Zemlya bays whereas populations living on the mainland can be expected to receive doses at least three orders of magnitude lower. PMID:10568274

Osvath, I; Povinec, P P; Baxter, M S

1999-09-30

398

Seasonal sea level cycle in the Caribbean Sea  

NASA Astrophysics Data System (ADS)

The seasonal sea level cycle has been investigated in the Caribbean Sea using altimetry and tide gauge time series from 27 stations and is characterized by large spatial variability. The coastal annual harmonic has amplitudes that range from 2 cm to 9 cm, peaking between August and October and semi-annual harmonic with maximum amplitude of 6 cm, with most stations peaking in April and October. The coastal seasonal sea level cycle contributes significantly at most areas to sea level variability and can explain the sea level variance up to 78%. The barometric effect on the coastal sea level seasonal cycles is insignificant in the annual component but dominant at 9 stations in the semi-annual cycle. The seasonal sea level cycle from 18 years of altimetry confirm the results obtained from the tide-gauges and allow us to identify some dominant sea level forcing parameters in the annual and semi-annual frequencies such as the Panama-Colombia gyre driven by the wind stress curl and the Caribbean Low Level Jet modulating the sea level in the northern coast of South America and linked to the local upwelling. The seasonal sea level cycle in the Caribbean Sea is unsteady in time, with large variations in amplitude and phase lag at most of the stations, where the 5-year amplitude in the coastal annual cycle can change over 6 cm in a 24 year period. The seasonal sea level cycle peaks about October when the probability of coastal impacts increases, especially in the northern coast of South America where the range is larger.

Torres, R. Ricardo; Tsimplis, Michael N.

2012-07-01

399

Arctic Sea Ice  

NASA Astrophysics Data System (ADS)

Of all the recent observed changes in the Arctic environment, the reduction of sea ice cover stands out most prominantly. Several independent analysis have established a trend in Arctic ice extent of -3% per decade from the late 1970s to the late 1990s, with a more pronounced trend in summer. The overall downward trend in ice cover is characterized by strong interannual variability, with a low September ice extent in one year typically followed by recovery the next September. Having two extreme minimum years, such as what was observed in 2002 and 2003 is unusual. 2004 marks the third year in a row of substantially below normal sea ice cover in the Arctic. Early summer 2004 appeared unusual in terms of ice extent, with May a record low for the satellite period (1979-present) and June also exhibiting below normal ice extent. August 2004 extent is below that of 2003 and large reductions in ice cover are observed once again off the coasts of Siberia and Alaska and the Greenland Sea. Neither the 2002 or 2003 anomaly appeared to be strongly linked to the positive phase of the Arctic Oscillation (AO) during the preceding winter. Similarly, the AO was negative during winter 2003/2004. In the previous AO framework of Rigor et al (2002), a positive winter AO implied preconditioning of the ice cover to extensive summer decay. In this hypothesis, the AO does not explain all aspects of the recent decline in Arctic ice cover, such as the extreme minima of 2002, 2003 and 2004. New analysis by Rigor and Wallace (2004) suggest that the very positive AO state from 1989-1995 can explain the recent sea ice minima in terms of changes in the Arctic surface wind field associated with the previous high AO state. However, it is also reasonable to expect that a general decrease in ice thickness accompanying warming would manifest itself as greater sensitivity of the ice pack to wind forcings and albedo feedbacks. The decrease in multiyear ice and attendant changes in ice thickness distribution could in turn precondition the Arctic ice cover to further reductions in the subsequent summer(s) regardless if the summer temperatures were anomalously warm. The NSIDC Sea Ice Index (http://nsidc.org/data/seaice_index/) can be used to view trends and anomalies from 1979 on.

Stroeve, J. C.; Fetterer, F.; Knowles, K.; Meier, W.; Serreze, M.; Arbetter, T.

2004-12-01

400

Hydrobiologia 420: 165184, 2000. A. M. Sole-Cava, C. A. M. Russo & J. P. Thorpe (eds), Marine Genetics.  

E-print Network

sponges to squid and include such diverse groups as sea cucumbers, barnacles, krill, octopuses, cuttlefish.g. barnacles, sponges, ascidians) or of very limited mobility (e.g. sea anemones, sea urchins, bivalves

Solé-Cava, Antonio M.

401

Isotope studies in the Caspian Sea  

Microsoft Academic Search

Oceanographic and isotopic investigations in the Caspian Sea and the analyses of the available data on the discharge to the sea and the observed sea level changes suggest that climatically caused changes of river inflow are the major cause of the sea level fluctuations over the last century. Hydrogen-3 and 3H–3He data indicate that the deep basins of the sea

K. Froehlich; K. Rozanski; P. Povinec; B. Oregioni; J. Gastaud

1999-01-01

402

The USGS Salton Sea Science Office  

USGS Publications Warehouse

The U.S. Geological Survey's (USGS) Salton Sea Science Office (SSSO) provides scientific information and evaluations to decisionmakers who are engaged in restoration planning and actions associated with the Salton Sea. The primary focus is the natural resources of the Salton Sea, including the sea?s ability to sustain biological resources and associated social and economic values.

Case, Harvey Lee, III; Barnum, Douglas A.

2007-01-01

403

Sea Otter, River Otter. The Wonder Series.  

ERIC Educational Resources Information Center

This curriculum guide is all about otters and provides information on both sea and river otters. Included are activities related to the diet of sea otters, the adaptations sea otters have made to live in the sea, their tool-using abilities, where they live and how to spot them, comparative anatomy of sea and river otters, and otter movement. The…

Robinson, Sandra Chisholm

404

Aerosols Over Yellow Sea Sediments  

NASA Technical Reports Server (NTRS)

This SeaWiFS image shows complex phytoplankton distribution patterns in the Bohai and Yellow seas. A wide band of brownish water along the coast north and south of the mouth of the Yangtze River indicates a heavy load of suspended sediment. The air over eastern central China and the Yellow Sea is thick with aerosols. Farther north over the Manchurian Plain and Greater Khingan Range, the air is much clearer.

2002-01-01

405

Sea Scallop Shell Lab Handout  

NSDL National Science Digital Library

Used in combination with the Sea Scallop Shell Lab teacher's guide, students will examine sea scallop shells to figure out as much as possible about the scallops living on the sea floor in one three important fishery grounds, Hudson Canyon, off New Bedford, MA, and George's Bank. The activity emphasizes observation, measurements, and basic calculations. The teacher's guide is available from the COSEE-NE OSEI resource site.

406

Sea Salt Source Function over the Baltic Sea  

NASA Astrophysics Data System (ADS)

Studies of production and transport of aerosol over the sea are very important for many areas of knowledge. Marine aerosol emitted from the sea surface helps to clean the boundary layer from other aerosol particles. The emitted droplets do not dry out in the highly humid surface layer air and because of their sizes most of them are deposited quickly at the sea surface. Therefore, marine aerosol has many features of rain i.e. the deposition in the marine boundary layer in high wind events is controlled not only by the "dry" processes but also by the "wet" scavenging. While many cruises conducted on board S/Y Oceania, we collected many data which were used to calculate sea salt source function over the Baltic Sea. Our cruises held between 2009 and 2012. Measurements were carried out using gradient method. For this method we used Laser Particle Counter (PMS model CSASP-100_HV) placed on one oft the mast of S/Y Oceania. Measurements were performed on five different levels around sea level: 8, 11, 14, 17 and 20 meters. Based on the averaged vertical concentration, profiles were calculated, using Monin-Obuchow theory, vertical sea spray fluxes in the near water layer. Based on fluxes calculated from vertical concentration profiles was calculated sea salt source function over the Baltic Sea. This function gives emission for different particle size, depending on environmental parameters. Emission of sea spray depends of the size of energy lost by the wind waves in process of collapse. Acknowledgements: The support for this study was provided by the project Satellite Monitoring of the Baltic Sea Environment - SatBa?tyk founded by European Union through European Regional Development Fund contract No. POIG 01.01.02-22-011/09.

Petelski, Tomasz; Makuch, Przemyslaw; Markuszewski, Piotr; Jankowski, Andrzej; Zieli?ski, Tymon

2013-04-01

407

Regime shifts in North Sea and Baltic Sea: A comparison  

NASA Astrophysics Data System (ADS)

The ICES subdivisions in the North Sea (SD IIIa, SD IVa, and SD IVb) and the subdivisions in the Baltic Sea (SD 29, SD 27/28-2, and SD 25/26) are selected to compare the response in long term monitoring data (1970-2000) with respect to climate regime shifts. A modified AMOEBA model is applied to the data sets to identify the status and development of the North Sea and Baltic Sea system during two recent regime shifts. Biological regime shifts can be identified 1989/1990 in SD IIIa in the North Sea and in SD 25/26 in the Baltic Sea. A synchronous appearance of regime shifts could only be identified in the central and southern Baltic Sea for both regime shifts 1975/76 and 1989/90 where the AMOEBA model indicated a high similarity in ecosystem response. A clear difference was identified in the response of the North Sea and the Baltic Sea. Inter-annual and inter-decadal variability as well as regime shifts are driven in the Baltic Sea by direct atmospheric forcing only. In contrast, the changes in the North Sea are influenced by both the direct atmospheric forcing and the indirect forcing from the changes in North Atlantic. The fact that regime shifts as well as their synchronous appearance can be identified with the AMOEBA model might be of major interest for the management of sustainable use of ecosystem goods and services, the development of ecosystem approach to management and the implementation of the Marine Strategy Framework Directive (MSFD) of the European Union (EU).

Dippner, Joachim W.; Möller, Caroline; Hänninen, Jari

2012-12-01

408

Polar Sea Ice Mapping Using SeaWinds Data Hyrum S. Anderson and David G. Long  

E-print Network

Polar Sea Ice Mapping Using SeaWinds Data Hyrum S. Anderson and David G. Long Brigham Young for mapping polar sea ice extent. In this study, a new al- gorithm for polar sea ice mapping is developed of Bayes detection to produce sea ice extent maps. Statistical models for sea ice and ocean are represented

Long, David G.

409

SeaWeb  

NSDL National Science Digital Library

SeaWeb is a project designed to raise awareness of the world's oceans and the lifeforms within them, and to encourage conservation efforts. Information provided here includes the latest news about ocean-related issues, audio files of the Ocean Report which provides a tour of the world's oceans, email updates, a bookstore, and an on-line book about issues facing our oceans. This includes habitats, fisheries and other issues. This site is searchable and provides links and resources for further information.

410

NOVA: Deep Sea Invasion  

NSDL National Science Digital Library

This is the companion Web site to "Deep Sea Invasion," a PBS NOVA documentary broadcast April 1, 2003. The program follows marine biologist Alexandre Meinesz and his scientific detective work to explain the rampant spread of the tropical alga Caulerpa taxifolia through the Mediterranean and his struggle to instigate control efforts. The features of this Web site include a timeline chronicling the invasion, an article by Meinesz on the impact of invasive species, another article addressing strategies for controlling invasives, and an interactive quiz in which users match up species with their invasive characteristics. With interesting material covering a range of ecological topics, this Web site should be of interest to any reader.

2003-01-01

411

Science Nation: Sea Turtles  

NSDL National Science Digital Library

Researchers at the University of North Carolina are studying how loggerhead and other sea turtles use the Earth's magnetic field for a journey of thousands of miles around the Atlantic Ocean. The turtles react to the Earth's magnetic field as they navigate a five-to-10-year journey that takes them from the east coast of the United States to the coast of Portugal, south toward the west coast of Africa, then back toward the beach where they were born. A better understanding of this turtle ability could help in research of both animal and human navigation.

412

The Sea Around Us  

NASA Astrophysics Data System (ADS)

Published in 1951, The Sea Around Us is one of the most remarkably successful books ever written about the natural world. Rachel Carson's rare ability to combine scientific insight with moving, poetic prose catapulted her book to first place on The New York Times best-seller list, where it enjoyed wide attention for thirty-one consecutive weeks. It remained on the list for more than a year and a half and ultimately sold well over a million copies, has been translated into 28 languages, inspired an Academy Award-winning documentary, and won both the 1952 National Book Award and the John Burroughs Medal. This classic work remains as fresh today as when it first appeared. Carson's writing teems with stunning, memorable images--the newly formed Earth cooling beneath an endlessly overcast sky; the centuries of nonstop rain that created the oceans; giant squids battling sperm whales hundreds of fathoms below the surface; and incredibly powerful tides moving 100 billion tons of water daily in the Bay of Fundy. Quite simply, she captures the mystery and allure of the ocean with a compelling blend of imagination and expertise. Reintroducing a classic work to a whole new generation of readers, this Special Edition features a new chapter written by Jeffrey Levinton, a leading expert in marine ecology, that brings the scientific side of The Sea Around Us completely up to date. Levinton incorporates the most recent thinking on continental drift, coral reefs, the spread of the ocean floor, the deterioration of the oceans, mass extinction of sea life, and many other topics. In addition, acclaimed nature writer Ann Zwinger has contributed a brief foreword. Today, with the oceans endangered by the dumping of medical waste and ecological disasters such as the Exxon oil spill in Alaska, this illuminating volume provides a timely reminder of both the fragility and the importance of the ocean and the life that abounds within it. Anyone who loves the sea, or who is concerned about our natural environment, will want to read this classic work.

Carson, Rachel L.

1991-12-01

413

Sea and Sky  

NSDL National Science Digital Library

This comprehensive site contains information about both astronomy and the oceans. The Sky section contains astronomical resources, news links, an image gallery, games and general links for more information. The Cosmos contains details about stars, planets, moons, pulsars, galaxies, black holes, quasars, star clusters, nebulas, dark matter and constellations. There is also a section about astronomers and a timeline of space exploration. The Sea section contains news links, aquarium resources, an image gallery, games and links for more information about the oceans. The Ocean Realm highlights the many creatures that live in the oceans. There are also details about ocean exploration and an exploration timeline.

Knight, J.

414

Lessons from Sea Launch  

NASA Astrophysics Data System (ADS)

This paper looks at the problems with space launch and what the United States can do to improve it. Specifically, the paper presents the argument that military space launch is not changing with the times and there are innovative ideas in the commercial launch sector, particularly the Sea Launch Program, from which the United States can learn. Primarily, the United States needs to make spacelift more affordable, reliable, and responsive because providing robust spacelift, that meets these standards, will support the US national security and economy. The research methodology included analysis of existing literature, congressional records, and interviews.

Cashin, Lina M.

2001-04-01

415

Two Sea-Level Challenges  

NASA Astrophysics Data System (ADS)

"No place on the sandy ocean shores of the world has been shown to be eroding because of sea level rise." This statement appeared nearly 19 years ago in bold print at the top of the page in a brief article published in Shore and Beach (Galvin,1990). The term "sea level rise" was defined in 1990 as follows: "In this statement, "sea level rise" has the meaning that the average person on the street usually attaches to that term. That is, sea level is rising; not, as in some places like the Mississippi River delta, land level is sinking." While still a subject of controversy, it is now (2008) increasingly plausible (Tornqvist et al,2008) that damage from Hurricane Katrina was significantly worse on the Mississippi River delta because floodwaters exploited wetlands and levees whose elevations had been lowered by decades of compaction in the underlying soil. (1) "Sea level" commonly appears in the literature as "relative sea level rise", occurring that way in 711 publications between 1980 and 2009 (GeoRef database on 8 Sep 08). "Relative sea level rise" does not appear in the 2005 AGI Glossary. The nearest Glossary term is "relative change in sea level", but that term occurs in only 12 publications between 1980 and 2009. The Glossary defines this term in a sequence stratigraphy sense, which infers that "relative sea level rise" is the sum of bottom subsidence and eustatic sea level rise. In plain English, "relative sea level rise" means "water depth increase". For present day coastal environments, "relative sea level rise" is commonly used where eustatic sea level rise is less than subsidence, that is, where the magnitude of actual sea level rise is smaller than the magnitude of subsidence. In that situation, "relative sea level rise" misleads both the average person and the scientist who is not a coastal geologist. Thus, the first challenge is to abandon "relative sea level rise" in favor of "water depth increase", in order that the words accurately descibe what happens. It would further clarify popular understanding if the term "actual sea level rise" were used in place of "eustatic sea level rise". (2)Geologists have approximated the the practice of paleontologists and biologists in establishing type examples of important geological features. This is a useful practice. A graduate geologist holds in mind clear conceptions of "beach cusps", "drumlin fields", "birdfoot deltas", and "igneous sills" based on seeing field examples accepted by professional geologists as representative of these features. However, although publications frequently report that sea level rise erodes a particular beach, no one identifies a type beach where that cause has been proven to produce the alleged effect. At the type beach, it is necessary to show that sea level is rising, and that the beach erodes primarily from this sea level rise, rather than from interrupted longshore transport. Thus, the second challenge is to identify a type ocean beach proven to erode because of sea level rise.

Galvin, C.

2008-12-01

416

Glacial sea surface temperature of the East Sea (Japan Sea) inferred from planktonic foraminiferal assemblage  

Microsoft Academic Search

In order to reconstruct the last glacial sea surface temperature (SST) of the East Sea, we investigated planktonic foraminiferal\\u000a assemblage in the marine sediments of a piston core recovered from the Ulleung Basin, East Sea. For core top, the most dominant\\u000a species is right coiling Neogloboquadrina incompta, while left coiling Neogloboquadrina pachyderma and Globigerina bulloides clearly dominate the glacial assemblages.

Kyung Eun Lee; Katsunori Kimoto; Dae Hyun Kim

2010-01-01

417

Spectacled Eiders Wintering in Northern Bering Sea  

USGS Multimedia Gallery

Large flock of Spectacled Eiders aggregated in a sea ice lead in the northern Bering Sea, south of St. Lawrence Island, Alaska.  Aerial photograph from a helicopter deployed from the USCG Cutter Polar Sea. ...

418

Spectacled Eiders Wintering in Northern Bering Sea  

USGS Multimedia Gallery

Large flock of Spectacled Eiders aggregated in a sea ice lead in the northern Bering Sea, south of St. Lawrence Island, Alaska.  Aerial photograph from a helicopter deployed from the USCG Cutter Polar Sea....

419

Spectacled Eiders Wintering in Northern Bering Sea  

USGS Multimedia Gallery

Large flock of Spectacled Eiders aggregated in a sea ice lead at sunset in the northern Bering Sea, south of St. Lawrence Island, Alaska.  Aerial photograph from a helicopter deployed from the USCG Cutter Polar Sea....

420

Carybdea marsupialis (Cubozoa) in the Mediterranean Sea: The First Case of a Sting Causing Cutaneous and Systemic Manifestations.  

PubMed

A woman stung by the box jellyfish Carybdea marsupialis (Cnidaria, Cubozoa) at a Spanish Mediterranean beach showed systemic manifestations over several months [pain far from the inoculation point, arthralgia, paresthesia, hyperesthesia, increase in eosinophils and immunoglobulin E (IgE)] in addition to the skin condition. PMID:25163356

Bordehore, Cesar; Nogué, Santiago; Gili, Josep-Maria; Acevedo, Melissa J; Fuentes, Verónica L

2015-01-01

421

Intraseasonal sea surface temperature variability in Indonesian seas  

NASA Astrophysics Data System (ADS)

The satellite-derived sea surface temperature (SST) data, 1998-mid 2012, are used to examine intraseasonal variability (ISV; 20-90 days) across the Indonesian seas. The most energetic ISV is observed in the Banda Sea and across the Indo-Australia basin with an The satellite-derived sea surface temperature (SST) data, 1998-mid 2012, are used to examine intraseasonal variability (ISV; 20-90 days) across the Indonesian seas. The most energetic ISV is observed in the Banda Sea and across the Indo-Australia basin with an average SST standard deviation (STD) between 0.4-0.5°C, with strongest signature during boreal winter. What physical processes force the SST ISV variability within the Indonesian seas? Ocean process, sea-air interaction, or both? To help identify the main forcing, the satellite derived outgoing longwave radiation (OLR) and wind stress data in the region are examined. The OLR shows robust intraseasonal variations and is significantly correlated with the SST, particularly for variability with periods of 30-60 days, with OLR accounting for ~60-70% of the SST variance. The OLR is also maximum during boreal winter. Conversely, the surface wind may play insignificant role in perturbing the SST at intraseasonal timescales as shown by weak correlation between wind stress and SST. We thus suspect that the surface solar flux (suggested by the OLR) is likely more dominant than the surface turbulent heat flux (indicated by the surface wind) as the main source for the ISV in the SST in Indonesian seas. Furthermore the maximum OLR phase, coupled with a period of minimum mixed layer depth, may explain the strong SST variation during boreal winter in Indonesian seas. The influence of the Madden-Julian Oscillation (MJO) on the OLR and SST variability is currently being evaluated.

Napitu, A. M.; Gordon, A. L.; Yuan, X.

2012-12-01

422

Ice in Caspian Sea and Aral Sea, Kazakhstan  

NASA Technical Reports Server (NTRS)

In this MODIS image from December 3, 2001, winter sea ice can be seen forming in the shallow waters of the northern Caspian (left) and Aral (upper right) Seas. Despite the inflow of the Volga River (upper left), the northern portion of the Caspian Sea averages only 17 ft in depth, and responds to the region's continental climate, which is cold in winter and hot and dry in the summer. The southern part of the Sea is deeper and remains ice-free throughout the winter. The dirty appearance of the ice may be due to sediment in the water, but may also be due to wind-driven dust. The wind in the region can blow at hurricane-force strength and can cause the ice to pile up in hummocks that are anchored to the sea bottom. The eastern portion of the Aral Sea is also beginning to freeze. At least two characteristics of the Aral Sea 'compete' in determining whether its waters will freeze. The Sea is shallow, which increases the likelihood of freezing, but it is also very salty, which means that lower temperatures are required to freeze it than would be required for fresh water. With average December temperatures of 18o F, it's clearly cold enough to allow ice to form. As the waters that feed the Aral Sea continue to be diverted for agriculture, the Sea becomes shallower and the regional climate becomes even more continental. This is because large bodies of water absorb and retain heat, moderating seasonal changes in temperature. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

2002-01-01

423

Sea modeling and rendering  

NASA Astrophysics Data System (ADS)

More and more defence and civil applications require simulation of marine synthetic environment. Currently, the "Future Anti-Surface-Guided-Weapon" (FASGW) or "anti-navire léger" (ANL) missile needs this kind of modelling. This paper presents a set of technical enhancement of the SE-Workbench that aim at better representing the sea profile and the interaction with targets. The operational scenario variability is a key criterion: the generic geographical area (e.g. Persian Gulf, coast of Somalia,...), the type of situation (e.g. peace keeping, peace enforcement, anti-piracy, drug interdiction,...)., the objectives (political, strategic, or military objectives), the description of the mission(s) (e.g. antipiracy) and operation(s) (e.g. surveillance and reconnaissance, escort, convoying) to achieve the objectives, the type of environment (Weather, Time of day, Geography [coastlines, islands, hills/mountains]). The paper insists on several points such as the dual rendering using either ray tracing [and the GP GPU optimization] or rasterization [and GPU shaders optimization], the modelling of sea-surface based on hypertextures and shaders, the wakes modelling, the buoyancy models for targets, the interaction of coast and littoral, the dielectric infrared modelling of water material.

Cathala, Thierry; Latger, Jean

2010-10-01

424

Sea ice terminology  

SciTech Connect

A group of definitions of terms related to sea ice is presented, as well as a graphic representation of late winter ice zonation of the Beaufort Sea Coast. Terms included in the definition list are belt, bergy bit, bight, brash ice, calving, close pack ice, compacting, compact pack ice, concentration, consolidated pack ice, crack, diffuse ice edge, fast ice, fast-ice boundary, fast-ice edge, first-year ice, flaw, flaw lead, floe, flooded ice, fractured, fractured zone, fracturing, glacier, grey ice, grey-white ice, growler, hummock, iceberg, iceberg tongue, ice blink, ice boundary, ice cake, ice edge, ice foot, ice free, ice island, ice shelf, large fracture, lead, medium fracture, multiyear ice, nilas, old ice, open pack ice, open water, pack ice, polar ice, polynya, puddle, rafted ice, rafting, ram, ridge, rotten ice, second-year ice, shearing, shore lead, shore polynya, small fracture, strip, tabular berg, thaw holes, very close pack ice, very open pack ice, water sky, young coastal ice, and young ice.

Not Available

1980-09-01

425

Greenland Sea observations  

SciTech Connect

ERS-1 SAR data have been acquired over the Greenland Sea and Fram Strait during two periods, the Ice Phase of three-day repeat cycle from January to March 1992 and a one-month period in the 35-day repeat cycle from 16 July to 15 August 1992. Most data became available by way of the Broadband Data Dissemination System, i.e. with a spatial resolution of about 100 m. With these data various algorithms have been tested to derive sea ice parameters such as ice extent, ice concentration and ice displacement. In the latter period data were collected to support the activities of a research vessel in the area mainly related to the large polynyas that form east and north of Greenland. The formation of polynyas could clearly be outlined but also other phenomena were observed related to the influence of wind streets and gravity waves associated with the atmospheric boundary layer. The data will have to be studied further including full-resolution data to substantiate the conclusions arrived at.

Gudmandsen, P.; Mortensen, H.B.; Pedersen, L.T.; Skriver, H. [Technical Univ. of Denmark, Lyngby (Denmark). Inst. of Electromagnetics; Minnett, P. [Brookhaven National Lab., Upton, NY (United States)

1992-12-31

426

Weibull-distributed sea clutter  

Microsoft Academic Search

Weibull-distributed sea clutter was measured using a fixed antenna of an L-band air-route surveillance radar at low grazing angles between 0.50 and 0.72 deg. It is shown that the sea-clutter amplitude statistics obey a Weibull distribution with a shape parameter of 1.585.

Matsuo Sekine; Toshimitsu Musha; Yuichi Tomita; Toshihiko Hagisawa; Takeru Irabu; Eiichi Kiuchi

1983-01-01

427

Weibull-distributed sea clutter  

NASA Astrophysics Data System (ADS)

Weibull-distributed sea clutter was measured using a fixed antenna of an L-band air-route surveillance radar at low grazing angles between 0.50 and 0.72 deg. It is shown that the sea-clutter amplitude statistics obey a Weibull distribution with a shape parameter of 1.585.

Sekine, M.; Musha, T.; Tomita, Y.; Hagisawa, T.; Irabu, T.; Kiuchi, E.

1983-08-01

428

Probability of sea level rise  

Microsoft Academic Search

The report develops probability-based projections that can be added to local tide-gage trends to estimate future sea level at particular locations. It uses the same models employed by previous assessments of sea level rise. The key coefficients in those models are based on subjective probability distributions supplied by a cross-section of climatologists, oceanographers, and glaciologists.

J. G. Titus; V. K. Narayanan

1995-01-01

429

Probability of sea level rise  

SciTech Connect

The report develops probability-based projections that can be added to local tide-gage trends to estimate future sea level at particular locations. It uses the same models employed by previous assessments of sea level rise. The key coefficients in those models are based on subjective probability distributions supplied by a cross-section of climatologists, oceanographers, and glaciologists.

Titus, J.G.; Narayanan, V.K.

1995-10-01

430

Evidence of Ice Free Seas  

NSDL National Science Digital Library

In this activity, students make a model sea floor sediment core using two types of buttons to represent fossil diatoms. They then compare the numbers of diatom fossils in the sediment at different depths to determine whether the seas were free of ice while the diatoms were alive.

LuAnn Dahlman

431

Connecting the Seas of Norden  

NASA Astrophysics Data System (ADS)

The Nordic Seas are highly sensitive to environmental change and have been extensively monitored and studied across a broad range of marine disciplines. For these reasons, the Nordic seas may serve as a pilot area for integrated policy development in response to ongoing climate change.

Paasche, Øyvind; Österblom, Henrik; Neuenfeldt, Stefan; Bonsdorff, Erik; Brander, Keith; Conley, Daniel J.; Durant, Joël M.; Eikeset, Anne M.; Goksøyr, Anders; Jónsson, Steingrímur; Kjesbu, Olav S.; Kuparinen, Anna; Stenseth, Nils Chr.

2015-02-01

432

Gallery: Sound in the Sea  

NSDL National Science Digital Library

Sound in the Sea offers a selection of audio recordings captured beneath the ocean surface. This page contains a selection of audio files of whales, ships, seismic disturbances, and unknown noises. There are also related video and animation products, and several spectrograms and other images of ocean sound. Students can click any image to listen and learn more about sound in the sea.

433

SEAS LABORATORY SAFETY OFFICER ORIENTATION  

E-print Network

/EH&S and lab · Communicate with lab members and PI · Get support from EH&S, ESCO and safety committees "The problems, and implement the safety program." SEAS Lab Safety Program Lab Safety Officer #12;· Attend monthly SEAS Safety Committee meetings · Assist in peer inspections and EH&S lab inspections · Inspect

434

SEA WATER RADIOLOGICAL MONITORING METHODS  

Microsoft Academic Search

The dispersal in time and sea of radioactive contamination produced by a ; deep underwater atomic blast was studied instrumentation methods obtained ; radiation intensity vs. depth information for several surface locations, and ; continuously monitored a sea-water intake line aboard ship. Surface ; contamination measuremerts were also made. (auth)

J. W. Duckworth; F. W. Chambers; W. H. Jr. Chapman; R. E. Severance

1958-01-01

435

The Barbados Sea Level Record  

NASA Astrophysics Data System (ADS)

Additional offshore drill cores, nearly 100 new radiometric dates, and more than 1000 kilometers of Multibeam mapping greatly enhance the Barbados Sea Level record. Extensive Multibeam mapping around the entire island covers approximately 2650 km2 of the sea bottom and now integrates the offshore reef topography and Barbados Sea Level Record with the unparalleled onshore core collection, digital elevation maps, and Pleistocene sea level record spanning the past one million years. The reef crest coral, Acropora palmata, remains the stalwart indicator of sea level for many reasons that are validated by our redundant sea level records and redundant dating via Th/U and Pa/U analyses. Microanalysis and densitometry studies better explain why Acropora palmata is so well preserved in the Pleistocene reef records and therefore why it is the species of choice for sea level reconstructions and radiometric dating. New drill cores into reefs that formed during Marine Isotope Stage 3 lead us to a model of diagenesis that allows us to better prospect for unaltered coral samples in older reefs that may be suitable for Th/U dating. Equally important, our diagenesis model reinforces our rigorous sample quality criteria in a more quantitative manner. The Barbados Sea Level record has a sampling resolution of better than 100 years throughout much of the last deglaciation showing unprecedented detail in redundant drill cores. The Melt Water Pulses (MWP1A and MWP1B) are well resolved and the intervening interval that includes the Younger Dryas reveals sea level changes in new detail that are consistent with the terrestrial records of ice margins (see Abdul et al., this section). More than 100 paired Th/U and radiocarbon ages place the Barbados Sea Level Record unambiguously on the radiocarbon time scale for direct comparisons with the terrestrial records of ice margin changes.

Fairbanks, R. G.; Mortlock, R. A.; Abdul, N. A.; Wright, J. D.; Cao, L.; Mey, J. L.

2013-12-01

436

ICESat measurements of sea ice freeboard and estimates of sea ice thickness in the Weddell Sea  

Microsoft Academic Search

Sea ice freeboard heights in the Weddell Sea of Antarctica are derived from the Ice, Cloud, and Land Elevation Satellite (ICESat) laser altimeter measurements, which have a unique range precision to flat surfaces of 2 cm within 70 m footprints spaced at 172 m along track. Although elevations of flat surfaces can be obtained to an accuracy of ?10 cm

H. Jay Zwally; Donghui Yi; Ron Kwok; Yunhe Zhao

2008-01-01

437

The Uptake of Dissolved Organic Matter by Juvenile Nematostella vectensis  

Microsoft Academic Search

Among marine invertebrates, nutrients can be acquired by consumption of particulate forms of food and through the absorption of organic molecules in seawater. We evaluated the ability of juvenile sea anemones (Nematostella vectensis) to take up dissolved organic matter (DOM) from seawater. ' As a cnidarian, the starlet sea anemone is diploblastic, composed of an endoderm, ectoderm, and an intervening

Yesensky Jessie; Hebron Allison

2008-01-01

438

Climate Projections of Sea state for the North Sea  

NASA Astrophysics Data System (ADS)

KLIWAS is a research program of the German Federal Ministry of Transport, Building and Urban Development to study the impacts of climate change on waterways and navigation and to provide options for adaptations. One aspect of the Research task is to analyse climate scenarios for the sea state, eg. Sea wave height (SWH), wave direction and wave periods for the North Sea. In addition, the prospective development of periods with wave heights below a certain threshold (periods of beneficial weather conditions) is discussed. Such periods of low sea state are important for offshore industry. The scenarios together with the wave climate of the recent years will give an approximation of projected changes of the sea state in coastal and open sea areas. Here we show the results for projected changes of sea state in the North Sea for the period 2000-2100 in comparison to 1961-2000, based on the wave model WAM4.5. The wave model is driven with wind data from two different regional atmosphere-ocean-models (DMI-HIRHAM and MPI-REMO) in the scenario A1B. The wind data are delivered in a horizontal resolution of about 20 km and a time resolution of one hour, while the wave model provides data of the calculated sea state with a horizontal grid of 5 km and the time resolution of one hour. It is seen, that in the eastern part of the North Sea and especially in the German Bight there is a trend to a increasing of the 99th percentile of SWH, in particular for the DMI wind data. In accordance with this increasing, there is a rotation of strongwind events from mainly north-west to mainly south-west directions for both regional models (DMI and REMO). As a consequence of this rotation, a decreasing of the 99th percentile of SWH is found in the western part of the North Sea. While there is a clear trend of SWH (positive in the eastern part, negative in the western part of the North Sea), there is not found any significant change of beneficial weather conditions.

Möller, Jens; Groll, Nikolaus; Heinrich, Hartmut; Rosenhagen, Gudrun

2013-04-01

439

Deep Sea Duel  

NSDL National Science Digital Library

This iOS app for the Illuminations online card game Deep Sea Duel (cataloged separately) helps users develop mental computation skills by finding sums of 3 or 4 numbers. A student and the opponent, Okta the octopus take turns selecting cards. The first one to reach the target sum with 3 cards (in the 9-card game) or 4 cards (in the 16-card game) wins the game. You can choose how many cards are presented (9 or 16), what types of numbers they display (small integers through tricky decimals), and Okta's level of strategy. The game is not timed but depends on strategic planning in order to defend against Okta's moves while trying to collect a winning group of cards.

2012-08-02

440

Deep Sea Duel  

NSDL National Science Digital Library

This Android app for the Illuminations online game Deep Sea Duel card game helps users develop mental computation skills by finding sums of 3 or 4 numbers. A student and the opponent, Okta the octopus take turns selecting cards. The first one to reach the target sum with 3 cards (in the 9-card game) or 4 cards (in the 16-card game) wins the game. You can choose how many cards are presented (9 or 16), what types of numbers they display (small integers through tricky decimals), and Okta's level of strategy. The game is not timed but depends on strategic planning in order to defend against Okta's moves while trying to collect a winning group of cards.

2012-08-29

441

Medicines for sea lice.  

PubMed

Sea louse (Family Caligidae: genera Caligus and Lepeophtheirus) infection of farmed salmonids represents a significant threat to animal welfare and undermines profitability. Lice may also act as vectors for the transmission of viral and bacterial pathogens. Pest-control programmes parallel those deployed in terrestrial livestock farming and include the use of parasiticides. The authorisation process for fish medicines varies widely between salmon farming countries and undue regulatory constraint may place farmers in one country at a competitive disadvantage. In many jurisdictions, fish are a 'minor' species and mounting demands for environmental assessment increase registration costs. A successful integrated louse-management strategy requires free access to a range of effective, chemically unrelated active ingredients deployed according to current best practice. Over-reliance on a limited number of products will lead, inevitably, to resistance, which is difficult to counter. PMID:12138618

Grant, Andrew N

2002-06-01

442

Kliwas - Climate Projections of Sea state for Coastal and Open Sea in the North Sea  

NASA Astrophysics Data System (ADS)

KLIWAS is a research program of the German Federal Ministry of Transport, Building and Urban Development to study the impacts of climate change on waterways and navigation and to provide options for adaptations. One specific aim of the project is to investigate potential changes in the wave fields in the North Sea. We have therefor analysed climate scenarios for the sea state, eg. significant wave height (SWH), wave direction and wave periods, for the North Sea. These scenarios together with the wave climate of the recent years will give an approximation of projected changes of the sea state in coastal and open sea areas. Here we show first results for projected changes of sea state in the North Sea for the period 2000-2100 in comparison to 1961-2000, based on the wave model WAM4.5. The wave model is forced with wind data from two different regional atmosphere-ocean-models (DMI-HIRHAM and MPI-REMO ) in the scenario A1B. The wind data have a horizontal resolution of about 20 km and a time resolution of one hour, while the wave model provides data of the calculated sea state with a horizontal grid of 5 km and the time resolution of one hour. We analysed the annual mean SWH as well as the 90- 95 and 99-percentile of SWH. It can be seen, that there is a trend to a slightly increasing SWH in the North Sea ,especially in the German Bight, in particular for the DMI wind data. While the increase is with the natural variability for the time period 2000-2050, it exceeds the variabiality in the second half of the century and shows a significant increase of SWH. The comparison with wave model runs for the scenarios A1 and B1 shows a similar increase of SWH, but a run with the scenario B2 displays no significant increase in the area of the German Bight and North Sea.

Möller, J.; Weisse, R.; Groll, N.; Heinrich, H.; Rosenhagen, G.

2012-04-01

443

Late Glacial to Holocene benthic foraminifera in the Marmara Sea: implications for Black Sea^Mediterranean Sea  

E-print Network

Late Glacial to Holocene benthic foraminifera in the Marmara Sea: implications for Black Sea^Mediterranean by the Mediterranean at V12 ka, allowing saline waters to penetrate the Marmara Sea. These saline waters reached Sea connections following the last deglaciation Michael A. Kaminski a;b;Ã? , Ali Aksu c , Matthew Box a

Kaminski, Michael A.

444

The mitochondrial 60-kDa heat shock protein in marine invertebrates: biochemical purification and molecular characterization  

PubMed Central

Sessile marine invertebrates undergo constant direct exposure to the surrounding environmental conditions, including local and global environmental fluctuations that may lead to fatal protein damage. Induction of heat shock proteins (Hsps) constitutes an important defense mechanism that protects these organisms from deleterious stress conditions. In a previous study, we reported the immunological detection of a 60-kDa Hsp (Hsp60) in the sea anemone Anemonia viridis (formerly called Anemonia sulcata) and studied its expression under a variety of stress conditions. In the present study, we show that the sponge Tetilla sp. from tidal habitats with a highly variable temperature regime is characterized by an increased level of Hsp60. Moreover, we show the expression of Hsp60 in various species among Porifera and Cnidaria, suggesting a general importance of this protein among marine invertebrates. We further cloned the hsp60 gene from A viridis, using a combination of conventional protein isolation methods and screening of a complementary deoxyribonucleic acid library by polymerase chain reaction. The cloned sequence (1764 bp) encodes for a protein of 62.8 kDa (588 amino acids). The 62.8-kDa protein, which contains an amino terminal extension that may serve as a mitochondrial targeting signal, shares a significant identity with mitochondrial Hsp60s from several animals but less identity with Hsp60s from either bacteria or plants. PMID:15270076

Choresh, Omer; Loya, Yossi; Müller, Werner E.G.; Wiedenmann, Jörg; Azem, Abdussalam

2004-01-01

445

The mitochondrial 60-kDa heat shock protein in marine invertebrates: biochemical purification and molecular characterization.  

PubMed

Sessile marine invertebrates undergo constant direct exposure to the surrounding environmental conditions, including local and global environmental fluctuations that may lead to fatal protein damage. Induction of heat shock proteins (Hsps) constitutes an important defense mechanism that protects these organisms from deleterious stress conditions. In a previous study, we reported the immunological detection of a 60-kDa Hsp (Hsp60) in the sea anemone Anemonia viridis (formerly called Anemonia sulcata) and studied its expression under a variety of stress conditions. In the present study, we show that the sponge Tetilla sp. from tidal habitats with a highly variable tempera