Sample records for sevier county tn

  1. Sevier-age ramp-style thrust faults at Cedar Mountain, northwestern San Rafael swell (Colorado Plateau), Emery County, Utah

    Microsoft Academic Search

    K. R. Neuhauser

    1988-01-01

    Field evidence, combined with published and unpublished seismic data, supports a new, inferred, easternmost limit of Sevier thrusting during Late Cretaceous foreland deformation in east-central Utah. Imbricate thrust faults are exposed along the western margin of Cedar Mountain in the northern part of the San Rafael swell within the western bounds of the Colorado Plateau. The faults offset Jurassic Entrada,

  2. 77 FR 24200 - American Drum & Pallet, Memphis, Shelby County, TN; Notice of Settlement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-23

    ...CERCLA-04-2012-3770, FRL-9663-8] American Drum & Pallet, Memphis, Shelby County, TN...response costs concerning the American Drum and Pallet Superfund Site located in Memphis...CERCLA-04- 2012-3770 or Site name American Drum & Pallet Superfund Site by one of the...

  3. Geological and petrophysical characterization of the Ferron Sandstone for 3-D simulation of a fluvial-deltaic reservoir. Deliverable 2.5.4, Ferron Sandstone lithologic strip logs, Emergy & Sevier Counties, Utah: Volume I

    SciTech Connect

    Allison, M.L.

    1995-12-08

    Strip logs for 491 wells were produced from a digital subsurface database of lithologic descriptions of the Ferron Sandstone Member of the Mancos Shale. This subsurface database covers wells from the parts of Emery and Sevier Counties in central Utah that occur between Ferron Creek on the north and Last Chance Creek on the south. The lithologic descriptions were imported into a logging software application designed for the display of stratigraphic data. Strip logs were produced at a scale of one inch equals 20 feet. The strip logs were created as part of a study by the Utah Geological Survey to develop a comprehensive, interdisciplinary, and qualitative characterization of a fluvial-deltaic reservoir using the Ferron Sandstone as a surface analogue. The study was funded by the U.S. Department of Energy (DOE) under the Geoscience/Engineering Reservoir Characterization Program.

  4. Public health assessment for USA Defense Depot Memphis, Memphis, Shelby County, Tennessee, Region 4. Cerclis No. TN4210020570. Final report

    SciTech Connect

    NONE

    1995-11-08

    The Defense Depot Memphis Tennessee (DDMT) is located on 642 acres in Memphis, Shelby County, Tennessee. In 1988, DDMT began to investigate soil and groundwater contamination. The chemicals contaminants that have been most found are volatile organic compounds (VOCs- mainly cleaners and solvents), petroleum products, pesticides, and metals. The contamination issues at DDMT that will be evaluated for public health implications are: contamination buried in Dunn Field (reaching the groundwater under that area); the possibility of contamination in drainage channels; the possibility of air contamination; and isolated areas of soil contamination on the Main Installation.

  5. Superfund record of decision amendment (EPA Region 4): Wrigley Charcoal Superfund Site, Hickman County, Wrigley, TN, February 2, 1995

    SciTech Connect

    NONE

    1995-03-01

    This decision document presents the selected Interim Remedial Action (IRA) for the Wrigley Charcoal Site, in Wrigley, Hickman County, Tennessee. The U.S. EPA has modified a wide variety of items that require immediate response action for the first step of cleanup activities to be taken at the Wrigley Charcoal Site. The major goal of these cleanup activities is to address the most serious threats at the Wrigley Charcoal Site by removing contaminated media from the Primary Site flood plain, remediating wastes at the Storage Basin, and through limited access restrictions at the Primary Site and the Storage Basin. The cleanup activities as presented in this IRA Record of Decision (ROD) Amendment will achieve significant risk reduction and will prepare the Site for future remedial activities.

  6. Ground-water hydrology of the Sevier Desert, Utah

    USGS Publications Warehouse

    Mower, R.W.; Feltis, R.D.

    1968-01-01

    The Sevier Desert, as used in this report, comprises the main part of the Sevier Desert, the Tintic Valley, and the southeastern part of the Old River Bed. It covers an area of about 3,000 square miles and occupies a large basin in the eastern part of the Basin and Range physiographic province. Large alluvial fans extend from the mountain fronts into the basin where they interfinger with eolian and lacustrine deposits and with fluvial deposits of the Sevier River. These unconsolidated deposits form a multiaquifer artesian system that is more than 1,000 feet thick and that extends from near the area of main recharge along the east side of the basin to Sevier Lake. Most of the recharge to the ground-water reservoir results from water entering alluvial fans as percolation from streams, irrigation ditches, and irrigated fields. Another important source may be water in the limestone, quartzite, and other consolidated rocks in the mountains that border the basin. Leakage from the Central Utah Canal is a major source of recharge to the water-table aquifer. Flowing wells are common in the central lowland part of the Sevier Desert, but as a result of below-normal precipitation and an increase in withdrawals from wells during 1950-64, the area of flowing wells has decreased. The quantity of ground water being wasted from flowing wells is not more than a few hundred acre-feet a year. The amount of water discharged by withdrawal from wells has increased nearly 15 times since 1950 (from 2,000 acre-feet in 1950 to 30,000 acre-feet in 1964). As a result of this increasing withdrawal, the water levels in observation wells have declined 4 feet in areas of small withdrawals to more than 7 feet near centers of pumping for public supplies and irrigation. An estimated 135,000-175,000 acre-feet of ground water is consumed by evapotranspiration each year in the 440,000 acres of desert that mainly support phreatophytes. This rate of discharge has changed little since 1950. The consumptive waste of ground water by undesirable phreatophytes, principally saltcedar and pickleweed, was not a serious problem in 1964 but could become a serious problem in the near future if saltcedar is permitted to spread. Water levels in wells changed little during 1935-40. During 1941-50, however, water levels rose in response to the general above-normal precipitation during 1939-47. During 1950-64 water levels declined, partly in response to below-normal precipitation and partly in response to an increase in pumping from irrigation wells. Although the period 1961-63 was one of above-normal precipitation, water levels continued the overall decline that was started in 1950. The decline, therefore, probably is due .to increased pumping. The amount of water that could be obtained from storage if the piezometric surface in the artesian aquifer were lowered 20 feet is estimated to be 120,006 acre-feet. The specific capacities of wells used for irrigation and public supply range from 5 to 215 gallons per minute per foot of drawdown. Specific capacities generally decrease with increasing distances away from the edge of the basin.

  7. 12. Deck view of bridge near Alum Cave parking area ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Deck view of bridge near Alum Cave parking area looking S. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

  8. Anatexis and metamorphism in tectonically thickened continental crust exemplified by the Sevier hinterland, western North America

    NASA Technical Reports Server (NTRS)

    Patino Douce, Alberto E.; Humphreys, Eugene D.; Johnston, A. Dana

    1990-01-01

    This paper presents a thermal and petrologic model of anatexis and metamorphism in regions of crustal thickening exemplified by the Sevier hinterland in western North America, and uses the model to examine the geological and physical processes leading to crustally derived magmatism. The results of numerical experiments show that anatexis was an inevitable end-product of Barrovian metamorphism in the thickened crust of the late Mesozoic Sevier orogenic belt and that the advection of heat across the lithosphere, in the form of mantle-derived mafic magmas, was not required for melting of metasedimentary rocks. It is suggested that, in the Sevier belt, as in other intracontinental orogenic belts, anatexis occurred in the midcrust and not at the base of the crust.

  9. New data on the late Pleistocene history of lake fluctuations in the Sevier Desert, Utah

    NASA Astrophysics Data System (ADS)

    Oviatt, C. G.; Spencer, J. Q.; Fan, Y.; Leggett, A.

    2010-12-01

    The chronology of lake-level changes during the late Pleistocene in the Lake Bonneville basin, Utah, is currently understood in a general way. Because the regressive phase of the lake occurred during a critical time in the history of the basin (climate was changing rapidly, humans were moving into suitable habitats that were being made available by the decline of the large lake), it is important to improve the dating of paleohydrologic and paleoclimatic events. We have initiated field and laboratory studies in a part of the Sevier Desert that was studied at a reconnaissance scale 25 to 30 years ago. Our work consists of describing critical stratigraphic sections in natural exposures along the Sevier River and in a core of lake and fluvial sediments, and in dating suitable materials from these sections using radiocarbon and optically stimulated luminescence (OSL). The Sevier basin was integrated with the rest of Lake Bonneville during the time when its highest shorelines were being formed, including during the long period of development of the Provo shoreline (~17.6-14.6 cal ka). The lake regressed rapidly from the Provo shoreline (~1465 m) to the altitude of the divide between the Sevier basin and the Great Salt Lake (GSL) basin (~1390 m), then split into two lakes. In the GSL basin the lake continued to decline as a closed-basin lake, and the lake in the shallow Sevier basin overflowed into it along a large river (the ancestral Sevier River). An extensive wetland, fed by runoff from the Sevier and Beaver Rivers, and by groundwater discharge, developed over much of the Sevier Desert floor during the early Holocene. We have measured new OSL ages that compare favorably with previously obtained radiocarbon ages for the same stratigraphic units. Part of our work is aimed at evaluating the hypothesis that rapid draw-downs in lake level would cause increased groundwater discharge to the lake, and therefore a potential radiocarbon reservoir effect that would have been spatially and temporally heterogeneous.

  10. Late Cretaceous extension in the hinterland of the Sevier thrust belt, northwestern Utah and southern Idaho

    Microsoft Academic Search

    Michael L. Wells; R. W. Allmendinger; R. D. Dallmeyer

    1990-01-01

    Cover rocks of the Raft River metamorphic core complex, located in the Sevier belt hinterland, preserve a structural and metamorphic history that predates the middle Tertiary extension of the region. In the eastern Raft River Mountains, Cambrian( )-Permian rocks form two allochthons that occur in the hanging wall of the mid-Miocene Raft River detachment fault. Dramatically attenuated, metamorphosed Cambrian( )-Pennsylvanian

  11. Inventory of wetlands and agricultural land cover in the upper Sevier River Basin, Utah

    NASA Technical Reports Server (NTRS)

    Jaynes, R. A.; Clark, L. D., Jr.; Landgraf, K. F. (principal investigator)

    1981-01-01

    The use of color infrared aerial photography in the mapping of agricultural land use and wetlands in the Sevier River Basin of south central utah is described. The efficiency and cost effectiveness of utilizing LANDSAT multispectral scanner digital data to augment photographic interpretations are discussed. Transparent overlays for 27 quadrangles showing delineations of wetlands and agricultural land cover were produced. A table summarizing the acreage represented by each class on each quadrangle overlay is provided.

  12. Paired, facing monoclines in the Sanpete-Sevier Valley area, central Utah

    USGS Publications Warehouse

    Witkind, I.J.

    1992-01-01

    Several major monoclines that trend northward through the Sanpete-Sevier Valley area of central Utah are paired and face one another. This pairing of monoclines may have occurred when near-horizontal sedimentary and volcanic strata subsided into voids created as salt was removed from a salt diapir concealed beneath valley fill. Removal was mostly by dissolution or extrusion during Neogene time. The paired monoclines, thus, are viewed as collapse features rather than as normal synclinal folds. -from Author

  13. Late Cretaceous extension in the hinterland of the Sevier thrust belt, northwestern Utah and southern Idaho

    Microsoft Academic Search

    Michael L. Wells; R. David Dallmeyer; Richard W. Allmendinger

    1990-01-01

    Cover rocks of the Raft River metamorphic core complex, located in the Sevier belt hinterland, preserve a structural and metamorphic history that predates the middle Tertiary extension of the region. In the eastern Raft River Mountains, Cambrian(?)-Permian rocks form two allochthons that occur in the hanging wall of the mid-Miocene Raft River detachment fault. Dramatically attenuated, metamorphosed Cambrian(?)-Pennsylvanian strata of

  14. Influence of preexisting tectonic trends on geometries of Sevier orogenic belt and its foreland in Utah

    SciTech Connect

    Picha, F.; Gibson, R.I.

    1985-05-01

    The tectonic style of the late Mesozoic Sevier orogenic belt in Utah was greatly affected by preexisting structural trends that date from the late Precambrian rifting and fragmentation of the North American continent. The late Precambrian cratonic margin (Cordilleran hinge line) was marked by a system of prominent faults including the north-south-trending ancestral Wasatch and ancient Ephraim faults and the southwest-northeast-trending Leamington, Scipio, Cove Fort, and Paragonah faults. During the Paleozoic and Mesozoic, renewed activity on these faults affected the geometries of the late Paleozoic Paradox and Oquirrh basins, the boundaries of the Jurassic Arapien Formation, and the sedimentary pattern of the Cretaceous foreland basin. Many of these fault zones were reactivated as tectonic ramps (e.g., the ancient Ephraim fault) and tear faults (e.g., the Leamington fault) during the compressional Sevier tectonism. The Fillmore arch and some other structural highs situated along the edge of the late Precambrian craton caused ramping of the inner Keystone-Pavant-Canyon thrust sheets and telescoping of the frontal thrust sheets. Post-thrust uplift of basement highs led to tectonic denudation and to the development of low-angle, extensional faults, such as the Sevier detachment. Northeast-trending lineaments, such as the Cove Fort and Paragonah lineaments, were reactivated as right-lateral strike-slip faults. They also affected the extent of the Marysvale volcanic field.

  15. 77 FR 17408 - Foreign-Trade Zone 204-Tri-Cities Area, TN/VA; Application for Reorganization Under Alternative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ...Med-Tech Parkway, Johnson City, Washington County, TN; Site 7 (103 acres, expires 6/30/15)--Linden/ Hairston Industrial Park, Linden Drive at Bonham Road, Bristol, Washington County, VA; Site 8 (2,100 acres, expires...

  16. 1. Small steel Girder Bridge with wooden railing looking WSW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Small steel Girder Bridge with wooden railing looking WSW. - Great Smoky Mountains National Park Roads & Bridges, Greenbrier Road, Between U.S. Route 321 & Greenbrier Road, Gatlinburg, Sevier County, TN

  17. 2. Big Creek Road, worm fence and road at trailhead. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Big Creek Road, worm fence and road at trailhead. - Great Smoky Mountains National Park Roads & Bridges, Big Creek Road, Between State Route 284 & Big Creek Campground, Gatlinburg, Sevier County, TN

  18. 1. View of Cades Cove Valley from first overlook on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Cades Cove Valley from first overlook on Rich Mountain Road looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  19. 19. Cantilevered barn in Cades Cove looking SSW. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Cantilevered barn in Cades Cove looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  20. 8. Double arch culvert on Laurel Creek Road looking WSW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Double arch culvert on Laurel Creek Road looking WSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  1. 5. Laurel Creek Road, revetment wall and creek. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Laurel Creek Road, revetment wall and creek. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  2. 12. Cades Cove Road, view toward entrance with ruts in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Cades Cove Road, view toward entrance with ruts in road. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  3. 14. Cades Cove Road, mountain view with single tree. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Cades Cove Road, mountain view with single tree. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  4. 6. 1930's revetment wall next to 1886 constructed revetment wall ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. 1930's revetment wall next to 1886 constructed revetment wall on Laurel Creek Road looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  5. 7. Elevation of single arch stone bridge on Laurel Creek ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Elevation of single arch stone bridge on Laurel Creek Road looking N. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  6. 15. Cades Cove Road, mountain view with horses. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Cades Cove Road, mountain view with horses. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  7. 18. View of fields, mountains and mist in Cades Cove ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of fields, mountains and mist in Cades Cove looking SW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  8. 17. Methodist Church in Cades Cove looking NW. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Methodist Church in Cades Cove looking NW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  9. 2. Switchback and General Road view looking S. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Switchback and General Road view looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  10. 25. Dan Lawson place in Cades Cove looking S. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. Dan Lawson place in Cades Cove looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  11. 13. Cades Cove Road, with barbed wire fence and mountain ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Cades Cove Road, with barbed wire fence and mountain view. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  12. 27. Abandoned treelined road in Cades Cove looking N. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. Abandoned tree-lined road in Cades Cove looking N. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  13. 10. Concrete bridge on road to Tremont looking S. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Concrete bridge on road to Tremont looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  14. 22. Cades Cove Valley from Hyatt Lane looking E. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Cades Cove Valley from Hyatt Lane looking E. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  15. 1. View of Laurel Creek Road, revetment wall and cliff ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Laurel Creek Road, revetment wall and cliff looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  16. 16. First Baptist Church in Cades Cove looking SSW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. First Baptist Church in Cades Cove looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  17. 2. View of west tunnel portal and stream on Laurel ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View of west tunnel portal and stream on Laurel Creek Road looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  18. 28. Ford near the end of Cades Cove Loop Road ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Ford near the end of Cades Cove Loop Road looking NNW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  19. 9. Double arch culvert on Laurel Creek Road looking ENE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Double arch culvert on Laurel Creek Road looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  20. 23. Henry Whitehead place in Cades Cove looking ESE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Henry Whitehead place in Cades Cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  1. 26. View of Cades Cove Loop Road with fields and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. View of Cades Cove Loop Road with fields and mountains looking NE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  2. Preliminary Feasibility Assessment of Geologic Carbon Sequestration Potential for TVA's John Sevier and Kingston Power Plants

    SciTech Connect

    Smith, Ellen D [ORNL; Saulsbury, Bo [ORNL

    2008-03-01

    This is a preliminary assessment of the potential for geologic carbon sequestration for the Tennessee Valley Authority's (TVA) John Sevier and Kingston power plants. The purpose of this assessment is to make a 'first cut' determination of whether there is sufficient potential for geologic carbon sequestration within 200 miles of the plants for TVA and Oak Ridge National Laboratory (ORNL) to proceed with a joint proposal for a larger project with a strong carbon management element. This assessment does not consider alternative technologies for carbon capture, but assumes the existence of a segregated CO{sub 2} stream suitable for sequestration.

  3. Late Cretaceous extension in the hinterland of the Sevier thrust belt, northwestern Utah and southern Idaho

    SciTech Connect

    Wells, M.L.; Allmendinger, R.W. (Cornell Univ., Ithaca, NY (USA)); Dallmeyer, R.D. (Univ. of Georgia, Athens (USA))

    1990-10-01

    Cover rocks of the Raft River metamorphic core complex, located in the Sevier belt hinterland, preserve a structural and metamorphic history that predates the middle Tertiary extension of the region. In the eastern Raft River Mountains, Cambrian( )-Permian rocks form two allochthons that occur in the hanging wall of the mid-Miocene Raft River detachment fault. Dramatically attenuated, metamorphosed Cambrian( )-Pennsylvanian strata of the lower allochthon yield Late Cretaceous muscovite {sup 40}Ar/{sup 39}Ar plateau ages (ca. 82-90 Ma) that are interpreted to date cooling during final stages of layer parallel extension. Devonian-Permian rocks exposed in the Black Pine Mountains underwent east-west extension ({approximately} 160%) associated with syntectonic growth of white mica along cleavage. {sup 40}Ar/{sup 39}Ar whole-rock spectra of associated slates also indicate a Late Cretaceous metamorphism. Together, field and {sup 40}Ar/{sup 39}Ar results suggest that Late Cretaceous extension occurred in the Sevier belt hinterland at the same time as shortening in the eastern foreland and at depth in the hinterland. Sufficient topography must have been present to drive upper-crustal extension in the eastern hinterland.

  4. Stratigraphy and structure of the Sevier thrust belt and proximal foreland-basin system in central Utah: A transect from the Sevier Desert to the Wasatch Plateau

    USGS Publications Warehouse

    Lawton, T.F.; Sprinkel, D.A.; Decelles, P.G.; Mitra, G.; Sussman, A.J.; Weiss, M.P.

    1997-01-01

    The Sevier orogenic belt in central Utah comprises four north-northwest trending thrust plates and two structural culminations that record crustal shortening and uplift in late Mesozoic and early Tertiary time. Synorogenic clastic rocks, mostly conglomerate and sandstone, exposed within the thrust belt were deposited in wedge-top and foredeep depozones within the proximal part of the foreland-basin system. The geologic relations preserved between thrust structures and synorogenic deposits demonstrate a foreland-breaking sequence of thrust deformation that was modified by minor out-of-sequence thrust displacement. Structural culminations in the interior part of the thrust belt deformed and uplifted some of the thrust sheets following their emplacement. Strata in the foreland basin indicate that the thrust sheets of central Utah were emplaced between latest Jurassic and Eocene time. The oldest strata of the foredeep depozone (Cedar Mountain Formation) are Neocomian and were derived from the hanging wall of the Canyon Range thrust. The foredeep depozone subsided most rapidly during Albian through Santonian or early Campanian time and accumulated about 2.5 km of conglomeratic strata (Indianola Group). The overlying North Horn Formation accumulated in a wedge-top basin from the Campanian to the Eocene and records propagation of the Gunnison thrust beneath the former foredeep. The Canyon Range Conglomerate of the Canyon Mountains, equivalent to the Indianola Group and the North Horn Formation, was deposited exclusively in a wedge-top setting on the Canyon Range and Pavant thrust sheets. This field trip, a three day, west-to-east traverse of the Sevier orogenic belt in central Utah, visits localities where timing of thrust structures is demonstrated by geometry of cross-cutting relations, growth strata associated with faults and folds, or deformation of foredeep deposits. Stops in the Canyon Mountains emphasize geometry of late structural culminations and relationships of the Canyon Range thrust to growth strata deposited in the wedge-top depozone. Stops in the San Pitch Mountains illustrate deposits of the foredeep depozone and younger, superjacent wedge-top depozone. Stops in the Sanpete Valley and western part of the Wasatch Plateau examine the evolution of the foreland-basin system from foredeep to wedge-top during growth of a triangle zone near the front of the Gunnison thrust.

  5. Ages of Sevier thrusting from dating of metamorphic garnet using the Lu-Hf method

    NASA Astrophysics Data System (ADS)

    Cruz-Uribe, A. M.; Hoisch, T. D.; Wells, M. L.; Vervoort, J. D.

    2008-12-01

    Combined thermodynamic modeling of garnet growth zoning and Lu-Hf dating of garnet yield well-constrained pressure-temperature-time (PTt) paths. Here we present PTt paths from amphibolite-facies pelitic garnet from the Raft River-Albion-Grouse Creek metamorphic core complex that constrain the timing of thrusting in the hinterland of the Sevier thrust belt. Three general times of thrust burial are indicated: 150, 138, and 85 Ma. Lu-Hf garnet dating of burial-related garnet growth in the Raft River Mountains yielded a Late Jurassic age of 149.9 ± 1.2 Ma (2?, MSWD = 1.1) based on three garnet fractions and a whole rock. A PT path from the schist of Mahogany Peaks in the Albion Range, Idaho, records an isothermal pressure increase indicating growth during thrusting. Lu-Hf dating of garnet from the same rock yielded an Early Cretaceous age of 138.7 ± 0.7 Ma (2?, MSWD = 1.6) based on seven garnet fractions. An additional PT path from a nearby outcrop also records an isothermal pressure increase and a similar Lu-Hf garnet age of 132.1 ± 5.1 Ma (2?, MSWD = 9.5) based on three garnet fractions and a whole rock. PT paths of multiple garnet grains from the schist of Stevens Spring in the Grouse Creek Mountains, Utah, exhibit isothermal pressure increases and yielded a Lu-Hf garnet age of 85.5 ± 1.9 Ma (2?, MSWD = 3.9) based on five garnet fractions and a whole rock. The Late Jurassic burial event recorded in the Raft River Mountains is older than the ages of inception of thrusting of the western thrusts of the Sevier fold-thrust belt including the Canyon Range and Paris - Willard thrusts, but consistent with an eastward progression in initial shortening in the orogenic wedge and development of an inferred thrust load responsible for the retroarc Morrison Formation basin. Early Cretaceous hinterland burial recorded in the Albion Range is permissively coeval with activity on the Willard and Canyon Range thrusts. Finally, renewed hinterland thrust burial during the Late Cretaceous, as documented in the Grouse Creek Mountains, is consistent in timing with previous interpretations of major thrusting in frontal thrust systems of the Sevier belt, such as the development of the Absaroka thrust.

  6. Role of crustal thickening and extensional collapse in the tectonic evolution of the Sevier-Laramide orogeny, western United States

    SciTech Connect

    Livaccari, R.F. (Univ. of New Mexico, Albuquerque (United States))

    1991-11-01

    The effects of crustal thickening and extensional collapse of continental crust, combined with variations in plate-convergence forces, provide a coherent explanation for evolution of the Cretaceous-early Tertiary Sevier-Laramide orogeny. Crustal thickening of the Sevier-Laramide hinterland region was triggered by the combination of compressive plate-convergence forces and subduction-induced conductive heating of the crust. Eastward progradation of the locus of Sevier-Laramide deformation through time reflects the progressive widening of the region of crustal thickening. The region of maximum crustal thickening migrated outward until about 80 to 75 Ma, when it encountered excessively strong lithosphere of the Colorado Plateau. The locus of deformation was then transmitted laterally across both a hinterland that had attained maximum crustal thickness and the rigid Colorado Plateau, into the Laramide Rocky Mountain foreland. An episode of vigorous extensional collapse of orogenically thickened crust affected the southern Cordillera between about 75 and 35 Ma. Early Tertiary waning of plate-convergence rates and continued subduction-induced conductive heating of thickened crust allowed extensional collapse along the southern Cordillera region to drive the Colorado Plateau block northward. The result was the late Laramide phase of Rocky Mountain foreland deformation in the central and southern Laramide Rocky Mountain region continued through Eocene time, rather than ceasing at 56 Ma, as it did in the northern Cordillera.

  7. Immunochemical studies on Tn erythrocyte glycoprotein.

    PubMed

    Lee, L T; Frank, S; de Jongh, D S; Howe, C

    1981-12-01

    Glycoproteins were extracted from membranes of erythrocytes that displayed Tn polyagglutination and were compared chemically and immunologically with glycoproteins of group O, MN cells. Tn glycoprotein had lower than normal NANA : protein and sugar : protein ratios, as revealed by direct analysis and polyacrylamide gel electrophoresis, and displayed slower immunoelectrophoretic mobility than glycoproteins of group O, MN cells. Agglutination of Tn cells by Salvia sclarea lectin was inhibited by Tn glycoprotein but not by O, MN glycoprotein. Tn and MN glycoproteins were equally potent inhibitors of influenza virus HA. Our findings indicate than Tn-specific determinants are part of the glycophorin molecule. PMID:6171313

  8. Transposition of Tn1000: in vivo properties.

    PubMed Central

    Tsai, M M; Wong, R Y; Hoang, A T; Deonier, R C

    1987-01-01

    Transposition mediated by the Tn1000 transposase was investigated by using transposon variants carrying synthetic or wild-type termini but no intact Tn1000 genes. Transposon Tn1001, whose only homologies to Tn1000 are in its 38-base-pair terminal inverted repeats, transposed at the same rate as Tn1005, an artificial construct carrying wild-type Tn1000 termini and approximately 1 kilobase of flanking Tn1000 DNA at each end, when transposase was supplied in trans. The majority of the transpositions into pOX38 gave rise to cointegrates, but approximately 10% of the products expressed phenotypes of direct transpositions. The expression and temperature dependence of the tnpA gene product were examined by studying transposition of Tn1001 to bacteriophage lambda. The temperature optimum for transposition was 37 degrees C, and the transposase was stable for up to 2 h at this temperature. Images PMID:2824438

  9. Characterization of Tn5801.Sag, a variant of Staphylococcus aureus Tn916 family transposon Tn5801 that is widespread in clinical isolates of Streptococcus agalactiae.

    PubMed

    Mingoia, Marina; Morici, Eleonora; Tili, Emily; Giovanetti, Eleonora; Montanari, Maria Pia; Varaldo, Pietro E

    2013-09-01

    Tn5801, originally detected in Staphylococcus aureus Mu50, is a Tn916 family element in which a unique int gene (int5801) replaces the int and xis genes in Tn916 (int916 and xis916). Among 62 tet(M)-positive tetracycline-resistant Streptococcus agalactiae isolates, 43 harbored Tn916, whereas 19 harbored a Tn5801-like element (Tn5801.Sag, ?20.6 kb). Tn5801.Sag was characterized (PCR mapping, partial sequencing, and chromosomal integration) and compared to other Tn5801-like elements. Similar to Tn5801 from S. aureus Mu50, tested in parallel, Tn5801.Sag was unable to undergo circularization and conjugal transfer. PMID:23817370

  10. Characterization of Tn5801.Sag, a Variant of Staphylococcus aureus Tn916 Family Transposon Tn5801 That Is Widespread in Clinical Isolates of Streptococcus agalactiae

    PubMed Central

    Mingoia, Marina; Morici, Eleonora; Tili, Emily; Giovanetti, Eleonora; Montanari, Maria Pia

    2013-01-01

    Tn5801, originally detected in Staphylococcus aureus Mu50, is a Tn916 family element in which a unique int gene (int5801) replaces the int and xis genes in Tn916 (int916 and xis916). Among 62 tet(M)-positive tetracycline-resistant Streptococcus agalactiae isolates, 43 harbored Tn916, whereas 19 harbored a Tn5801-like element (Tn5801.Sag, ?20.6 kb). Tn5801.Sag was characterized (PCR mapping, partial sequencing, and chromosomal integration) and compared to other Tn5801-like elements. Similar to Tn5801 from S. aureus Mu50, tested in parallel, Tn5801.Sag was unable to undergo circularization and conjugal transfer. PMID:23817370

  11. A Fold Province in the Hinterland of the Sevier Orogenic Belt in Eastern Nevada: A Valley and Ridge in the Basin and Range

    NASA Astrophysics Data System (ADS)

    Long, S. P.

    2014-12-01

    The overprinting effects of Cenozoic extensional tectonism hinder analysis of the style, geometry, and timing of Cordilleran contractional deformation in the hinterland of the Sevier fold-thrust belt in Nevada. To address this problem, a 1:250,000-scale paleogeologic map of eastern Nevada, showing spatial distributions of Paleozoic-Mesozoic rocks exposed beneath a Paleogene unconformity, divided out at the formation scale, is integrated with maps of dip magnitude for Paleozoic-Mesozoic and Tertiary rocks, published sedimentary thickness records, and a published reconstruction of Basin and Range extension, in order to define and regionally-correlate thrust faults and folds, and estimate the pre-extensional amplitude, wavelength, and limb dips of folds. A new structural province, the Eastern Nevada fold belt (ENFB) is defined, and consists of a 100-150 km-wide region containing as many as five 1st-order, N-trending folds that can be traced for map distances between 100-250 km, have amplitudes between 2-4 km, wavelengths between 20-40 km, pre-extensional limb dips typically between 10-30?, and deform rocks as young as Jurassic and Early Cretaceous. No regional-scale thrust faults or decollemont horizons breach modern exposure levels in the ENFB. ENFB folds are interpreted to have formed above a deep (?10 km below the Paleogene unconformity), blind decollemont or shear zone, perhaps the westward projection of the master decollemont of the Sevier fold-thrust belt. Two additional structural provinces of the Sevier hinterland, the Central Nevada thrust belt (CNTB) and Western Utah thrust belt (WUTB), represent zones of N-striking, E-vergent thrust faults and folds that each accommodated ca. 10 km of shortening. Both thrust belts branch northward off of the Sevier thrust belt, and are interpreted to represent contemporary, interior components of the Sevier thrust system. The CNTB, WUTB, and the intervening ENFB collectively record low-magnitude (a few 10's of km) upper-crustal shortening that accompanied Cretaceous detachment and translation of the Cordilleran passive margin basin 220 km eastward during Sevier orogenesis. Low deformation magnitudes in the Sevier hinterland are attributed to the high rheological competence of this thick basin.

  12. The Structure and Properties of Functional Titanium Nickelide-Based TN-10, TN-20, and TN-1V Alloys

    NASA Astrophysics Data System (ADS)

    Khodorenko, V. N.; Kaftaranova, M. I.; Gunther, V. É.

    2015-05-01

    The interrelation among the structural features, martensitic transformation characteristics, and physical-mechanical properties of functional titanium nickelide-based TN-10, TN-20, and TN-1V alloys is investigated. Martensitic transformations in a TN-10 alloy at temperatures of 273 and 298 K are found to develop at lower martensite shear stresses than in TN-20 and TN-1V alloys. This is due to the internal structure formed in the production of the material. The degree of ultimate strain and the magnitude of ultimate stress for each of the alloys depend on the state of the matrix at a pre-assigned deformation temperature and on the contribution of the martensitic transformation mechanisms of deformation under applied load. The structure formed and the plastic component of deformation are shown to exert a dramatic effect on the type and characteristics of fracture.

  13. Cretaceous to early Tertiary paleogeography in the hinterland of the Sevier thrust belt, east-central Nevada

    SciTech Connect

    Vandervoort, D.S.; Schmitt, J.G. (Montana State Univ., Bozeman (USA))

    1990-06-01

    The Cretaceous to early Tertiary hinterland of the Sevier thrust belt in east-central Nevada underwent two periods of sedimentary basin development. The older (Early Cretaceous) episode is characterized by basins that formed coeval with deformation within a belt of contractional structures. Basins were characterized by periods of through-flowing fluvial drainage. The younger (Late Cretaceous to Eocene) episode of basin development was characterized by internally drained alluvial basins that subsequently expanded into more regional lacustrine basins. Basal alluvial detritus indicates that basin inception occurred as a result of rapid development of topographic relief. Synsedimentary tectonism was characterized by horizontal extension due to attainment of equilibrium topography in response to crustal thickening.

  14. Detection and characterization of Tn2501, a transposon included within the lactose transposon Tn951.

    PubMed Central

    Michiels, T; Cornelis, G

    1984-01-01

    The DNA sequence spanning coordinates 9.9 to 16.4 kilobases of the lactose transposon Tn951 ( Cornelis et al., Mol. Gen. Genet. 160:215-224, 1978) constitutes a transposable element by itself. Unlike Tn951 ( Cornelis et al., Mol. Gen. Genet. 184:241-248, 1981), this element, called Tn2501 , transposes in the absence of any other transposon. Transposition of Tn2501 proceeds through transient cointegration and duplicates 5 base pairs of host DNA. Tn2501 is flanked by nearly perfect inverted repeats (44 of 48), related to the inverted repeats of Tn21 ( Zheng et al., Nucleic Acids Res. 9:6265-6278, 1982). Unlike Tn21 , Tn2501 does not confer mercury resistance. Images PMID:6327643

  15. Characterization of Tn 10d Cam: A transposition-defective Tn 10 specifying chloramphenicol resistance

    Microsoft Academic Search

    Thomas Elliott; John R. Roth

    1988-01-01

    We have constructed a small, transposition-defective derivative of the transposon Tn10 that carries the chloramphenicol acetyltransferase gene of pACYC184. This new genetic element, Tn10d-Cam, transposes when Tn10 transposase is provided from a multi-copy plasmid. Transposon insertion mutagenesis of Salmonella typhimurium was performed by using a strain carrying a Tn10d-Cam insertion in an Escherichia coli F' episome as the donor in

  16. SHORT REPORT Open Access Insertion site preference of Mu, Tn5, and Tn7

    E-print Network

    Boyer, Edmond

    , Mu, Tn5, Mutagenesis, Insertion site, DNA transposon, Mobile element Background Transposons, mobile be a confounding factor for large scale transposon mutagenesis projects. A number of manuscripts reportingSHORT REPORT Open Access Insertion site preference of Mu, Tn5, and Tn7 transposons Brian Green1

  17. Technical Note TN-56 Power and Energy

    E-print Network

    Flinn, Jason

    WRL Technical Note TN-56 Power and Energy Characterization of the Itsy Pocket Computer (Version 1 Alto, California 94301 USA #12;WRL TECHNICAL NOTE TN- 1 Research group. Our focus is research on information technology that is relevant to the technical strategy

  18. Transposition of Tn7 in Pseudomonas aeruginosa and Isolation of alk::Tn7 Mutations

    PubMed Central

    Fennewald, Michael A.; Shapiro, James A.

    1979-01-01

    Conjugal crosses with Pseudomonas aeruginosa donors carrying the CAM-OCT and RP4::Tn7 plasmids result in transfer of the Tn7 trimethoprim resistance (Tpr) determinant independently of RP4 markers. All Tpr exconjugants which lack RP4 markers have CAM-OCT genes and therefore must have received CAM-OCT::Tn7 plasmids formed by transposition of Tn7 from RP4::Tn7 to CAM-OCT. Most crosses yield exconjugants carrying mutant CAM-OCT plasmids which no longer determine either camphor or alkane utilization and thus appear to carry Tn7 inserts in the cam or alk loci, respectively. Transduction and reversion experiments indicated that at least 13 alkane-negative, camphor-positive, Tpr CAM-OCT::Tn7 plasmids carry an alk::Tn7 mutation. Determination of linkage between the alk mutation and the Tpr determinant of Tn7 on these plasmids is complicated by the presence of multiple copies of the Tn7 element in the genome. Generalized transduction will remove Tn7 from a CAM-OCT alk::Tn7 plasmid to yield alk+ cells which carry no Tpr determinant on the CAM-OCT plasmid (as shown by transfer of the plasmid to a second strain). But the transduction to alk+ does not remove all Tpr determinants from the genome of the recipient cell because the alkane-positive transductants remain trimethoprim resistant. Thus, it appears that copies of Tn7 can accumulate in the genome of P. aeruginosa (CAM-OCT alk::Tn7) strains without leaving their original site. This result is consistent with transposition models that involve replication of the transposable element without excision from the original site. PMID:110782

  19. Mucin associated Tn and sialosyl-Tn antigen expression in colorectal polyps

    Microsoft Academic Search

    S H Itzkowitz; E J Bloom; T S Lau; Y S Kim

    1992-01-01

    Sialosyl-Tn antigen and its immediate precursor, Tn antigen, are carbohydrate structures associated with the earliest steps of mucin O-linked glycosylation. Both antigens have been shown previously to be highly sensitive and specific markers of colorectal cancer. One hundred and three colorectal polyps (79 adenomatous; 24 hyperplastic) were examined for expression of Tn antigen using vicia villosa isolectin B4, and for

  20. Tn polyagglutinability occurring in a patient with B cell lymphoma

    Microsoft Academic Search

    M. Wallner; R. Waldner

    1985-01-01

    A case of polymorphic immunocytoma (B cell lymphoma) coinciding with expression of Tn antigen on a population of erythrocytes is presented. Tn activation was found incidentally by screening blood samples of patients suffering from hematologic malignancies with a Tn specific lectin from Salvia sclarea. So far, Tn activation has been reported only in apparently healthy subjects or in subjects suffering

  1. Paleogeographic and paleotectonic setting of sedimentary basins in the Sevier thrust belt and hinterland, eastern Great Basin

    SciTech Connect

    Schmitt, J.G. (Montana State Univ., Bozeman, MT (United States). Dept. of Earth Sciences); Vandervoort, D.S. (Cornell Univ., Ithaca, NY (United States). Dept. of Geological Sciences); Suydam, J.D. (Washington State Univ., Pullman, WA (United States). Dept. of Geology)

    1993-04-01

    The eastern Great Basin contains a sparse record of broadly distributed Cretaceous sedimentary rocks which record: evolution of intermontane basins during development of the Sevier (Sv)contractional orogen and incipient extensional collapse of the elevated Sv hinterland (east-central NV), and complex tectono-sedimentary interactions between frontal thrust belt structures and the western margin of the adjacent foreland basin. Palinspastic restoration of these strata and associated structures to pre-Tertiary extension positions reveals a clearer pictures of Cretaceous basin paleogeography and allows comparison with the Puna/Altiplano plateau and precordillera thrust belt of the Neogene Andean orogen. Two syntectonic stratal assemblages are present in east-central NV. Lower Cretaceous alluvial strata (Newark Canyon Fm) record basin development coeval with emergence of contractional structures in the Sv hinterland. Localized early Cretaceous basins were possibly piggyback immature; periods of open drainage to the to the east and south suggest connection with the nascent Sv foreland basin to the east (Cedar Mountain/Sanpete Fms) prior to major thrust loading in central Utah. Development of hinterland structures is almost recorded by Aptian-Albian foreland basin alluvial deposits in SW Utah (Dakota Fm) and southern Nevada (Willow Tank Fm). Upper Cretaceous to Eocene strata (Sheep Pass Fm) record inception of regionally abundant alluvial-lacustrine basins which developed in response to onset of latest Cretaceous extension and associated collapse of the Sv hinterland. Evolution of the structurally complex western margin of the Sv foreland basin is recorded in Cretaceous through Eocene strata deposited in: piggyback basins which were at times hydrologically connected to the adjacent foreland basins, and thrust-proximal portions of the foreland basin. These proximal areas are characterized by folding and faulting of basin fill and development of intrabasinal unconformities.

  2. 19. Photographer unknown, circa 1950 'THE LOOP OVER' BRIDGE WAS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Photographer unknown, circa 1950 'THE LOOP OVER' BRIDGE WAS CONSTRUCTED ON NEWFOUND GAP ROAD TO REPLACE A SERIES OF DANGEROUS SWITCHBACKS. THIS ENGINEERING FEATURE QUICKLY BECAME A POPULAR TOURIST ATTRACTION. - Great Smoky Mountains National Park Roads & Bridges, Gatlinburg, Sevier County, TN

  3. COMPUTER ENG 4TN4 Image Processing

    E-print Network

    Haykin, Simon

    COMPUTER ENG 4TN4 Image Processing Academic year 2010-2011, term 2 Instructor: Xiaolin Wu, ITB-A315 for developing applications and for research in the field of image processing. · Provide training for the design and implementation of practical algorithms for image processing. Outline of Topics: · Introduction o Applications

  4. Tn polyagglutinability occurring in a patient with B cell lymphoma.

    PubMed

    Wallner, M; Waldner, R

    1985-11-01

    A case of polymorphic immunocytoma (B cell lymphoma) coinciding with expression of Tn antigen on a population of erythrocytes is presented. Tn activation was found incidentally by screening blood samples of patients suffering from hematologic malignancies with a Tn specific lectin from Salvia sclarea. So far, Tn activation has been reported only in apparently healthy subjects or in subjects suffering from or developing myeloid leukemia. PMID:3933597

  5. Structural and microstructural evolution of the Rattlesnake Mountain Anticline (Wyoming, USA): New insights into the Sevier and Laramide orogenic stress build-up in the Bighorn Basin

    NASA Astrophysics Data System (ADS)

    Beaudoin, Nicolas; Leprêtre, Rémi; Bellahsen, Nicolas; Lacombe, Olivier; Amrouch, Khalid; Callot, Jean-Paul; Emmanuel, Laurent; Daniel, Jean-Marc

    2012-11-01

    The Rocky Mountains in western US provide among the best examples of thick-skinned tectonics: following a period of thin-skinned tectonics related to the Sevier orogeny, the compressional reactivation of basement faults gave birth to the so-called Laramide uplifts/arches. The Bighorn basin, located in Wyoming, is therefore a key place to study the transition from thin- to thick-skinned tectonics in orogenic forelands, especially in terms of microstructural and stress/strain evolution. Our study focuses on a classic Laramide structure: the Rattlesnake Mountain Anticline (RMA, Wyoming, USA), a basement-cored anticline located in the western part of the Bighorn basin. Stress and strain evolution analysis in folded sedimentary layers and underlying faulted basement rocks were performed on the basis of combined analyses of fractures, fault-slip data and calcite twinning paleopiezometry. Most of the fractures are related to three main tectonic events: the Sevier thin-skinned contraction, the Laramide thick-skinned contraction, and the Basin and Range extension. Serial balanced cross-sections of RMA and displacement profiles suggest that all thrust faults were coeval, evidencing strain distribution in the basement during faulting. The comparison of RMA with another structure located in the eastern edge of the Bighorn basin, i.e. the Sheep Mountain Anticline (SMA), allows to propose a conceptual model for the geometric and kinematic evolution of Laramide-related basement-cored anticlines. Finally, the stress evolution is reconstructed at both the fold scale and the basin scale. We show that the evolution of stress trends and magnitudes was quite similar in both structures (RMA and SMA) during Laramide times (thick-skinned tectonics), in spite of different stress regimes. During Sevier (thin-skinned tectonics) and post-Laramide times, stress trends and fracture patterns were different in these two structures. These results suggest that the distance to the orogenic front influenced the fracture patterns but not the foreland stress magnitudes, which were likely controlled by the structural style.

  6. Geologic Map of the Pahranagat Range 30' x 60' Quadrangle, Lincoln and Nye Counties, Nevada

    USGS Publications Warehouse

    Jayko, A.S.

    2007-01-01

    Introduction The Pahranagat Range 30' x 60' quadrangle lies within an arid, sparsely populated part of Lincoln and Nye Counties, southeastern Nevada. Much of the area is public land that includes the Desert National Wildlife Range, the Pahranagat National Wildlife Refuge, and the Nellis Air Force Base. The topography, typical of much of the Basin and Range Province, consists of north-south-trending ranges and intervening broad alluvial valleys. Elevations range from about 1,000 to 2,900 m. At the regional scale, the Pahranagat Range quadrangle lies within the Mesozoic and early Tertiary Sevier Fold-and-Thrust Belt and the Cenozoic Basin and Range Province. The quadrangle is underlain by a Proterozoic to Permian miogeoclinal section, a nonmarine clastic and volcanic section of middle Oligocene or older to late Miocene age, and alluvial deposits of late Cenozoic age. The structural features that are exposed reflect relatively shallow crustal deformation. Mesozoic deformation is dominated by thrust faults and asymmetric or open folds. Cenozoic deformation is dominated by faults that dip more than 45i and dominostyle tilted blocks. At least three major tectonic events have affected the area: Mesozoic (Sevier) folding and thrust faulting, pre-middle Oligocene extensional deformation, and late Cenozoic (mainly late Miocene to Holocene) extensional deformation. Continued tectonic activity is expressed in the Pahranagat Range area by seismicity and faults having scarps that cut alluvial deposits.

  7. ATP-dependent specific binding of Tn3 transposase to Tn3 inverted repeats

    NASA Astrophysics Data System (ADS)

    Wishart, W. L.; Broach, J. R.; Ohtsubo, E.

    1985-04-01

    Transposons are discrete segments of DNA which are capable of moving from one site in a genome to many different sites1,2. Tn3 is a prokaryotic transposon which is 4,957 base pairs (bp) long and encodes a transposase protein which is essential for transposition3-7. We report here a simple method for purifying Tn3 transposase and demonstrate that the transposase protein binds specifically to the ends of the Tn3 transposon in an ATP-dependent manner. The transposase protein binds to linear double-stranded DNA both nonspecifically and specifically; the nonspecific DNA binding activity is sensitive to challenge with heparin. Site-specific DNA binding to the ends (inverted repeats) of Tn3 is observed only when binding is performed in the presence of ATP; this ATP-dependent site-specific DNA binding activity is resistant to heparin challenge. Our results indicate that ATP qualitatively alters the DNA binding activity of the transposase protein so that the protein is able to bind specifically to the ends of the Tn3 transposon.

  8. 76 FR 29284 - Tennessee Disaster #TN-00055

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ...Economic Injury (EIDL) Loan Application Deadline Date: 02/09/2012. ADDRESSES: Submit completed loan applications to: U.S...disaster: Primary Counties: Benton, Carroll, Crockett, Dyer, Gibson, Henderson, Henry, Houston, Lake, Lauderdale,...

  9. Abundance of Tn3, Tn21, and Tn501 transposase (tnpA) sequences in bacterial community DNA from marine environments.

    PubMed Central

    Dahlberg, C; Hermansson, M

    1995-01-01

    The occurrence of the tnpA genes of the transposons Tn3, Tn21, and Tn501 was assessed in total bacterial community DNA isolated from different marine environments. The PCR technique was employed, together with most probable number statistics, to determine the abundance of the target tnpA genes. All three genes could be detected, and the Tn21 tnpA sequences predominated in all samples. The smallest amount of total community DNA in which the Tn21 tnpA sequence could be detected was 0.037 ng, and on the basis of our results, we estimated that this sequence was present in 1 of 1,000 to 10,000 bacteria. Hybridization of the PCR products with the respective tnpA probes verified the Tn21 and Tn501 tnpA sequences but only some of the Tn3 tnpA amplification products. The distribution and dissemination of transposons in natural bacterial communities are discussed. PMID:7487037

  10. Development of new cancer vaccines derived from sTn

    Microsoft Academic Search

    Jian Wu

    2007-01-01

    sTn is a tumor-associated carbohydrate antigen (TACA) expressed in many types of human carcinoma. It is thus an important target for the development of new cancer therapies. Some sTn-based vaccines have been clinically examined for treating cancer, but the problem of immune tolerance of patients to sTn has severely hindered the clinical efficacy. To overcome this problem and develop useful

  11. Transposable elements Updated March 29, 2012 4 (none) Tpm kan (Tn903) Tn903 pkan none none --K CDE Epicentre

    E-print Network

    Borenstein, Elhanan

    Transposable elements Updated March 29, 2012 Vector(s)d Reference 4 (none) Tpm kan (Tn903) Tn903 pkan none none - - K CDE Epicentre (none) Tpm tp (dhfr ) Tn7 pdhfr none none - - N/D N/D Epicentre T1 TnphoA phage TnphoA Transduction kan ? phoA TL none - - H ABC [1] T2 TnlacZ phage Tnlac

  12. Functional characterization of Tn4401, a Tn3-based transposon involved in blaKPC gene mobilization.

    PubMed

    Cuzon, Gaelle; Naas, Thierry; Nordmann, Patrice

    2011-11-01

    The carbapenemase gene bla(KPC), which is rapidly spreading worldwide, is located on a Tn3-based transposon, Tn4401. In a transposition-conjugation assay, Tn4401 was able to mobilize bla(KPC-2) gene at a frequency of 4.4 × 10(-6)/recipient cell. A 5-bp target site duplication was evidenced upon each insertion without target site specificity. This study demonstrated that Tn4401 is an active transposon capable of mobilizing bla(KPC) genes at high frequency. PMID:21844325

  13. Magnitude, Timing, and Geometry of Extension in the Southern Sevier Desert Basin From Piercing Points, Seismic-Stratigraphic Reconstruction, and Deep well Data

    NASA Astrophysics Data System (ADS)

    Coogan, J. C.; Decelles, P. G.

    2007-12-01

    Palinspastic reconstruction of Mesozoic thrust sheets provides the main constraint for an estimated 47 km of Cenozoic extensional displacement along the Sevier Desert detachment (SDD) in the central Sevier Desert Basin. Hanging wall and footwall piercing points indicate that the SDD accommodated a minimum of 35 km of extensional displacement in the narrower southern part of the basin. The piercing points for the SDD are defined by the intersection of the SDD, the Canyon Range thrust (CRT), and a regional early Cenozoic erosion surface (ES). The hanging wall piercing point lies immediately northeast of the Cricket Mountains, where the SDD-CRT- ES intersection is narrowly defined by intersecting structure maps derived from published seismic reflection data. The footwall piercing point lies in the southern foothills of the Canyon Range, where the SDD breakaway plane is well constrained by an industry seismic line that lies within 2 km of the exposed intersection of the CRT with the base of the Oligocene Oak City Formation. Timing of extension in the southern Sevier Desert basin is constrained by a kinematic reconstruction of detachment and imbricate fault displacement, footwall uplift, and supradetachment sedimentation for Oligocene, Miocene, and Plio-Pleistocene seismic sequences. The reconstruction is centered on a seismic reflection and gravity interpretation along the published Pan Canadian profiles 2 and 3 that is tied to dated intervals in six industry wells. Fault restoration indicates that Oligocene and Miocene phases of slip each accounted for about 40 percent of the total displacement. Simultaneous backstripping of the Oligocene, Miocene, and Plio-Pleistocene supradetachment sequences records hanging wall subsidence simultaneous with footwall uplift, with a footwall burial history that is consistent with published Miocene apatite and zircon fission-track ages of footwall samples. The geometric evolution of the southern SDD extensional system is consistent with its development above a broad westward-migrating "rolling hinge" zone associated with isostatic uplift of the detachment footwall. Hanging wall normal faults east of the footwall crest exhibit small post-Miocene displacement, with demonstrable Quaternary slip restricted to the crest and western limb of the uplift, most notably along the Black Rock and Clear Lake fault zones. Early abandonment of the eastern part of the detachment may explain the indistinct geomorphic and structural expression of the break-away zone at the surface. The deepest level of the southern SDD also presents a complex geometry and kinematic history. The 1996 Chevron 1-29 Black Rock Federal well through the western basin margin penetrated a normal fault that places Jurassic over lower Cambrian strata at 4650 m measured depth, well above the principal SDD seismic reflection. The fault is not correlated to any large- displacement high-angle fault at shallow levels, and may form the abandoned roof to an extensional duplex.

  14. Genomic analysis of insertion behavior and target specificity of mini-Tn7 and Tn3 transposons in

    E-print Network

    Gerstein, Mark

    for insertion mutagenesis, large-scale gene disruption studies and gene tagging (1). Transposon-mediated geneGenomic analysis of insertion behavior and target specificity of mini-Tn7 and Tn3 transposons Transposons are widely employed as tools for gene disruption. Ideally, they should display unbiased insertion

  15. Surface plasmon resonance and NMR analyses of anti Tn-antigen MLS128 monoclonal antibody binding to two or three consecutive Tn-antigen clusters.

    PubMed

    Matsumoto-Takasaki, Ayano; Hanashima, Shinya; Aoki, Ami; Yuasa, Noriyuki; Ogawa, Haruhiko; Sato, Reiko; Kawakami, Hiroko; Mizuno, Mamoru; Nakada, Hiroshi; Yamaguchi, Yoshiki; Fujita-Yamaguchi, Yoko

    2012-03-01

    Tn-antigens are tumour-associated carbohydrate antigens that are involved in metastatic processes and are associated with a poor prognosis. MLS128 monoclonal antibody recognizes the structures of two or three consecutive Tn-antigens (Tn2 or Tn3). Since MLS128 treatment inhibits colon and breast cancer cell growth [Morita, N., Yajima, Y., Asanuma, H., Nakada, H., and Fujita-Yamaguchi, Y. (2009) Inhibition of cancer cell growth by anti-Tn monoclonal antibody MLS128. Biosci. Trends 3, 32-37.], understanding the interaction between MLS128 and Tn-clusters may allow us to the development of novel cancer therapeutics. Although MLS128 was previously reported to have specificity for Tn3 rather than Tn2, similar levels of Tn2/Tn3 binding were unexpectedly observed at 37°C. Thus, thermodynamic analyses were performed via surface plasmon resonance (SPR) using synthetic Tn2- and Tn3-peptides at 10, 15, 20, 25 and 30°C. SPR results revealed that MLS128's association constants for both antigens were highly temperature dependent. Below 25°C MLS128's association constant for Tn3-peptide was clearly higher than that for Tn2-peptide. At 30°C, however, the association constant for Tn2-peptide was higher than that for Tn3-peptide. This reversal of affinity is due to the sharp increase in K(d) for Tn3. These results were confirmed by NMR, which directly measured MLS128-Tn binding in solution. This study suggested that thermodynamic control plays a critical role in the interaction between MLS128/Tn2 and MLS128/Tn3. PMID:22161472

  16. The catalytic residues of Tn3 resolvase.

    PubMed

    Olorunniji, Femi J; Stark, W Marshall

    2009-12-01

    To characterize the residues that participate in the catalysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase, we mutated conserved polar or charged residues in the catalytic domain of an activated resolvase variant. We analysed the effects of mutations at 14 residues on proficiency in binding to the recombination site ('site I'), formation of a synaptic complex between two site Is, DNA cleavage and recombination. Mutations of Y6, R8, S10, D36, R68 and R71 resulted in greatly reduced cleavage and recombination activity, suggesting crucial roles of these six residues in catalysis, whereas mutations of the other residues had less dramatic effects. No mutations strongly inhibited binding of resolvase to site I, but several caused conspicuous changes in the yield or stability of the synapse of two site Is observed by non-denaturing gel electrophoresis. The involvement of some residues in both synapsis and catalysis suggests that they contribute to a regulatory mechanism, in which engagement of catalytic residues with the substrate is coupled to correct assembly of the synapse. PMID:19789272

  17. ERDC TN-DOER-E27 October 2010

    E-print Network

    US Army Corps of Engineers

    ERDC TN-DOER-E27 October 2010 The Relationship Between Acoustic Target Strength and Body Length: This technical note presents the results of field measurements of acoustic target strength (TS) for Atlantic

  18. NCAR/TN-480+STR NCAR TECHNICAL NOTE

    E-print Network

    NCAR/TN-480+STR NCAR TECHNICAL NOTE ________________________________________________________________________ April 2010 Technical Description of an Urban Parameterization for the Community Land Model (CLMU) Keith Edition 2153-2400 #12;NCAR TECHNICAL NOTES http

  19. Expression of a Tn-like epitope by carcinoma cells.

    PubMed Central

    Roxby, D. J.; Skinner, J. M.; Morley, A. A.; Weeks, S.; Burpee, M.

    1987-01-01

    A monoclonal antibody, FBT3, was raised against Tn positive erythrocytes and, using immunohistochemistry, fresh and fixed tissues from patients with cancer were studied to detect any expression of a Tn-like epitope. Expression was found in neoplastic cells, usually both in cytoplasm and on cell membranes, from 104 of 147 cases of carcinoma and 1 of 13 cases of lymphoma, but rarely in adjacent, morphologically normal cells. Tn expression was seen in some normal glandular cells but, unlike cancer cells, it was distributed as fine granules in a supranuclear position. Detection of a Tn-like epitope is of theoretical interest and may be of direct diagnostic value. Images Figure 1 Figure 2 Figure 3 PMID:2449235

  20. TnT -- A Statistical Part-of-Speech Tagger

    Microsoft Academic Search

    Thorsten Brants

    2000-01-01

    Trigrams'n'Tags (TnT) is an efficient statistical part-of-speech tagger. Contrary to claims found elsewhere in the literature, we argue that a tagger based on Markov models performs at least as well as other current approaches, including the Maximum Entropy framework. A recent comparison has even shown that TnT performs significantly better for the tested corpora. We describe the basic model of

  1. Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9

    Microsoft Academic Search

    N. Kirby Alton; Daniel Vapnek

    1979-01-01

    The transposable genetic element Tn9 consists of two direct repeats of the insertion sequence IS1 flanking a region of 1,102 base pairs which determines chloramphenicol resistance1,2. Transposition of Tn9 leads to the duplication of a 9-base pair sequence which preexists at the site of insertion. One copy of this sequence is found at each end of the inserted element3. The

  2. 78 FR 52424 - Amendment of Class E Airspace; Dayton, TN, Establishment of Class E Airspace; Cleveland, TN, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-23

    ...establishes Class E Airspace at Cleveland Regional Jetport, Cleveland, TN, to accommodate area navigation (RNAV) global positioning system (GPS) SIAPs at the airport. Information regarding Bradley Memorial Hospital is added to the Cleveland,...

  3. Vertical transmission of TnSNPV, TnCPV, AcMNPV, and possibly recombinant NPV in Trichoplusia ni

    E-print Network

    Hammock, Bruce D.

    (TnCPV), Autographa californica nucleopolyhedrovirus (AcMNPV), and AcMNPV engineered to express). All rights reserved. Keywords: Trichoplusia ni; Nucleopolyhedrovirus; Trichoplusia ni NPV; Autographa californica NPV; Recombinant NPV; Cypovirus; Trichoplusia ni CPV; Transmission, viral; Transmission, vertical

  4. Transposon mutagenesis in halophilic eubacteria: conjugal transfer and insertion of transposon Tn5 and Tn 1732 in Halomonas elongata

    Microsoft Academic Search

    Hans Jörg Kunte; Erwin A. Galinski

    1995-01-01

    Molecular genetic studies of halophilic eubacteria have been limited by the lack of a suitable method for mutagenesis. To overcome this, we established a transposon mutagenesis procedure for the ectoine-producing, halophilic bacterium Halomonas elongata. We used suicide plasmids pSUP101 and pSUP102-Gm to introduce the transposons Tn5 and Tn7732 respectively into H. elongata via Escherichia coli SM10 mediated conjugation. Our finding

  5. Transposon mutagenesis in Desulfovibrio desulfuricans: Development of a random mutagenesis tool from Tn7

    SciTech Connect

    Wall, J.D.; Murnan, T.; Argyle, J. [Univ. of Missouri, Columbia, MO (United States)] [and others

    1996-10-01

    The transposons Tn5, Tn7, Tn9, and Tn10 or their derivatives have been examined for transposition in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20. Tn7 inserted with a frequency of 10{sup {minus}4} to 10{sup {minus}3} into a unique attachment site that shows strong homology with those sites identified in other gram-negative bacteria. Inactivation of the tnsD gene in Tn7, encoding the function directing insertion into the unique site, yielded a derivative that transposed essentially randomly with a frequency of ca. 10{sup {minus}6} per donor. Derivatives of Tn5, but not wild-type Tn5, were also found to undergo random transposition at a similar frequency. No evidence was obtained for transposition of Tn9 or Tn10. 34 refs., 5 figs., 2 tabs.

  6. 76 FR 32387 - Tennessee Disaster Number TN-00053

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-06

    ...08/2011. EIDL Loan Application Deadline Date: 02/09/2012. ADDRESSES: Submit completed loan applications to: U.S...Primary Counties: (Physical Damage and Economic Injury Loans): Gibson, Lauderdale. Contiguous Counties: (Economic Injury...

  7. 75 FR 29590 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-26

    ...following areas as adversely affected by the disaster: Primary Counties: (Physical Damage and Economic Injury Loans): Cannon, Giles, Marshall. Contiguous Counties: (Economic Injury Loans Only): Alabama: Limestone. Tennessee: Lincoln. All other...

  8. TN 3.1b, Version 1.1, 07-Oct-2011 AE-TN-KNMI-GS-0031b-v1 1

    E-print Network

    Haak, Hein

    TN 3.1b, Version 1.1, 07-Oct-2011 AE-TN-KNMI-GS-0031b-v1 1 TN3.1b Test results for the L2B processor Reference: AE-TN-KNMI-GS-0031b-v1 1 Version 1.1, dated 07-Oct-2011 Authors Jos de Kloe, Ad Stoffelen, Gert-Jan Marseille, KNMI Alain Dabas M´et´eo-France Dorit Huber DoRiT 1/ 575 #12;TN 3.1b, Version

  9. The Lower Sevier River Basin Crop Monitor and Forecast Decision Support System: Exploiting Landsat Imagery to Provide Continuous Information to Farmers and Water Managers

    NASA Astrophysics Data System (ADS)

    Torres-Rua, A. F.; Walker, W. R.; McKee, M.

    2013-12-01

    The last century has seen a large number of innovations in agriculture such as better policies for water control and management, upgraded water conveyance, irrigation, distribution, and monitoring systems, and better weather forecasting products. In spite of this, irrigation management and irrigation water deliveries by farmers/water managers is still based on factors like water share amounts, tradition, and past experience on irrigation. These factors are not necessarily related to the actual crop water use; they are followed because of the absence of related information provided in a timely manner at an affordable cost. Thus, it is necessary to develop means to deliver continuous and personalized information about crop water requirements to water users/managers at the field and irrigation system levels so managers at these levels can better quantify the required versus available water for irrigation during the irrigation season. This study presents a new decision support system (DSS) platform that addresses the absence of information on actual crop water requirements and crop performance by providing continuous updated farm-based crop water use along with other farm performance indicators such as crop yield and farm management to irrigators and water managers. This DSS exploits the periodicity of the Landsat Satellite Mission (8 to 16 days, depending on the period of interest) to provide remote monitoring at the individual field and irrigation system levels. The Landsat satellite images are converted into information about crop water use, yield performance and field management through application of state-of-the-art semi-physical and statistical algorithms that provide this information at a pixel basis that are ultimately aggregated to field and irrigation system levels. A version of the DSS has been implemented for the agricultural lands in the Lower Sevier River, Utah, and has been operational since the beginning of the 2013 irrigation season. The main goal of this DSS implementation is to provide continuous and personalized information to farmers and water managers regarding crops in fields and the irrigation delivery system throughout the irrigation season so that decisions related to agricultural water use can result in water savings while not diminishing crop yields.

  10. NCAR/TN-487+STR NCAR TECHNICAL NOTE

    E-print Network

    1 NCAR/TN-487+STR NCAR TECHNICAL NOTE 2153-2397 ISSN Electronic Edition 2153-2400 #12;2 NCAR TECHNICAL NOTES http://www.ucar.edu/library/collections/technotes/technotes.jsp The Technical Notes series provides an outlet for a variety of NCAR Manuscripts that contribute in specialized

  11. Evaluation of TnT Tagger for Spanish

    Microsoft Academic Search

    Raul Morales Carrasco; Alexander F. Gelbukh

    2003-01-01

    Part of speech (POS) tagger is a necessary module in many natural language text processing tasks. A POS tagger is a program that accepts an unprepared raw text in input and to each word adds a tag specifying its grammatical properties, such as part of speech, number, person, etc. One of popular POS taggers - TnT tagger - has been

  12. Powers in arithmetic progress (III) T.N. Shorey

    E-print Network

    Shorey, T.N.

    Powers in arithmetic progress (III) T.N. Shorey in arithmetic progression This is in contiuation of [30] and [31]. For an integer > 1, we denote result is due to Euler [see [18]) that a product of four terms in arithmetic progression

  13. 75 FR 27846 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ...Robertson, Smith, Stewart, Trousdale, Wayne, Wilson. Contiguous Counties: (Economic Injury Loans Only): Tennessee: Overton, Pickett, Putnam, Warren, White. Alabama: Lauderdale. Kentucky: Calloway, Clinton, Cumberland, Logan,...

  14. 75 FR 27008 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ...Contiguous Counties: (Economic Injury Loans Only): Kentucky: Allen, Simpson. Tennessee: Bedford, Cannon, Coffee, Giles, Lawrence, Macon, Trousdale. All other information in the original declaration remains unchanged. (Catalog of...

  15. 75 FR 32201 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ...climate change to the Service's wildlife mission in environmental education programs and pursue opportunities for carbon sequestration with native trees. Alternative D would continue to manage cultural resources consistent with Section 106 of...

  16. 76 FR 5194 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN; Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ...303 species of birds, and 280 species of mammals, fish, reptiles, and amphibians. We announce our decision and the availability...and implement baseline inventories for non-game mammals, reptiles, amphibians, fish, and invertebrates. We will also...

  17. National Association of Counties

    NSDL National Science Digital Library

    2002-01-01

    With 3066 U.S. counties, the National Association of Counties (NACo) has the arduous task of making sure the nations counties are acknowledged and understood in the halls of the White House and Congress. NACo, the only national government organization devoted to highlighting and improving the understanding of county issues, "collects, researches, publishes and disseminates a variety of different information for, on and about counties." On their Web site, viewers have access to a comprehensive and in-depth database of county information including county population; census bureau quick facts; elected county officials; county codes and ordinances; county policies; links to Capitolimpact.com, which provides nationwide county statistics such as economic and demographic data; and much more. This site is easily navigable, has counties arranged alphabetically by state, and would be of value to anyone living inside a county domain --- which is just about everyone.

  18. Renewed interest in basic and applied research involving monoclonal antibodies against an oncofetal Tn-antigen.

    PubMed

    Fujita-Yamaguchi, Yoko

    2013-08-01

    Tn-antigen (GalNAc?-Ser/Thr) is one of the most common aberrations associated with cancer progression and metastasis, and thus is an excellent target for development of cancer diagnostics and therapeutics. MLS128 monoclonal antibody (mAb), derived from a mouse immunized with human colon carcinoma cells, was reported to bind to two or three consecutive Tn-antigens (Tn2 or Tn3) with one-order higher affinity for Tn3 than for Tn2. Our recent studies demonstrated that MLS128 significantly inhibits breast and colon cancer cell growth. Molecular cloning of the variable regions of heavy (VH) and light (VL) chains revealed that the VH sequence of MLS128 shared 97% nucleotide sequence identity with the VH of 83D4 mAb, derived from breast cancer-immunized mice, which has a similar affinity for Tn2/Tn3. MLS128 single-chain antibodies (scFv) and scFv-Fc were constructed to confirm the affinity for synthetic Tn2/Tn3 peptides. Thermodynamic studies on MLS128 binding to Tn2/Tn3 revealed its unique nature of temperature-dependent binding. PMID:23740330

  19. TnT --A Statistical Part-of-Speech Tagger Thorsten Brants

    E-print Network

    Wicentowski, Richard

    TnT -- A Statistical Part-of-Speech Tagger Thorsten Brants Saarland University Computational Linguistics D-66041 Saarbriicken, Germany thorst en@coli, uni-sb, de Abstract Trigrams'n'Tags (TnT the Maximum Entropy framework. A recent comparison has even shown that TnT performs significantlybetter

  20. Hydration of the dTn·dAn·dTn parallel triple helix: a Fourier transform infrared and gravimetric study correlated with molecular dynamics simulations

    Microsoft Academic Search

    Mohammed Ouali; Hervé Gousset; Frédéric Geinguenaud; Jean Liquier; Jacques Gabarro-Arpa; Marc Le Bret; Eliane Taillandier

    We present a comparative analysis of the water organization around the dTn·dAn·dTn triple helix and the Watson-Crick double helix dTn·dAn respectively by means of gravimetric measurements, infrared spectro- scopy and molecular dynamics simulations. The hydration per nucleotide determined by gravimetric and spectroscopic methods correlated with the molecular dynamics simulations shows that at high relative humidity (98% RH) the triple helix

  1. Topological vertex for Higgsed 5d $T_N$ theories

    E-print Network

    Hirotaka Hayashi; Gianluca Zoccarato

    2015-05-01

    We analyse the computation of the partition function of 5d $T_N$ theories in Higgs branches using the topological vertex. The theories are realised by a web of $(p,q)$ 5-branes whose dual description may be given by an M-theory compactification on a certain local non-toric Calabi-Yau threefold. We explicitly show how it is possible to directly apply the topological vertex to the non-toric geometry. Using this novel technique, which considerably simplifies the computation by the existing method, we are able to compute the partition function of the higher rank $E_6$, $E_7$ and $E_8$ theories. Moreover we show how in some specific cases similar results can be extended to the computation of the partition function of 5d $T_N$ theories in the Higgs branch using the refined topological vertex. These cases require a modification of the refined topological vertex.

  2. p2 and inhibition of Tn5 transposition.

    PubMed Central

    Yin, J C; Reznikoff, W S

    1988-01-01

    Mutations of Tn5 which decreased the amount of the shorter element-encoded protein (p2) were made. One mutation was a change in the translation initiation codon of the protein, while two other mutations were changes in the promoter of the transcript (T2) which codes for p2. Analysis of all three mutants indicates that they decreased the inhibition of transposition that the protein exerts (in trans) on another element. The mutants have complicated transposition behaviors. Analysis of the RNA and proteins synthesized from the mutants led to the proposal that p2 can inhibit transposition at normal physiological concentrations. Therefore p2 synthesized from a given element is partly responsible for controlling the transposition frequency of the element. The mutants also show that p1 is the only Tn5-encoded protein necessary for transposition. Images PMID:2838454

  3. Organization of the Tn6-related kanamycin resistance transposon Tn2680 carrying two copies of IS26 and an IS903 variant, IS903. B.

    PubMed

    Mollet, B; Clerget, M; Meyer, J; Iida, S

    1985-07-01

    The kanamycin resistance transposon Tn2680, which originates from the R plasmid Rts1, is homologous to Tn6 and carries two directly repeated copies of IS26, one at each end. The kanamycin resistance gene codes for type I aminoglycoside-3'-phosphotransferase. Tn2680 also contains, in the middle of the transposon, an additional IS element homologous to IS903. This element, designated IS903.B, is flanked by a 9-base-pair direct target duplication. A novel kanamycin resistance transposon. Tn2681, can be generated from Tn2680 by IS903.B-mediated cointegration and subsequent reciprocal recombination between the directly repeated IS26 sequences. Tn2681 carries a single IS26 element in the middle of the transposon and is flanked by two directly repeated copies of IS903.B. Possible evolutionary relationships between Tn2680 and other kanamycin resistance transposons such as Tn903 and Tn2350 are discussed, based on the gene organization and DNA sequences. PMID:2989253

  4. Organization of the Tn6-related kanamycin resistance transposon Tn2680 carrying two copies of IS26 and an IS903 variant, IS903. B.

    PubMed Central

    Mollet, B; Clerget, M; Meyer, J; Iida, S

    1985-01-01

    The kanamycin resistance transposon Tn2680, which originates from the R plasmid Rts1, is homologous to Tn6 and carries two directly repeated copies of IS26, one at each end. The kanamycin resistance gene codes for type I aminoglycoside-3'-phosphotransferase. Tn2680 also contains, in the middle of the transposon, an additional IS element homologous to IS903. This element, designated IS903.B, is flanked by a 9-base-pair direct target duplication. A novel kanamycin resistance transposon. Tn2681, can be generated from Tn2680 by IS903.B-mediated cointegration and subsequent reciprocal recombination between the directly repeated IS26 sequences. Tn2681 carries a single IS26 element in the middle of the transposon and is flanked by two directly repeated copies of IS903.B. Possible evolutionary relationships between Tn2680 and other kanamycin resistance transposons such as Tn903 and Tn2350 are discussed, based on the gene organization and DNA sequences. Images PMID:2989253

  5. Catalysis of site-specific recombination by Tn3 resolvase.

    PubMed

    Olorunniji, Femi J; Stark, W Marshall

    2010-04-01

    The active-site interactions involved in the catalysis of DNA site-specific recombination by the serine recombinases are still incompletely understood. Recent crystal structures of synaptic gammadelta resolvase-DNA intermediates and biochemical analysis of Tn3 resolvase mutants have provided new insights into the structure of the resolvase active site, and how interactions of the catalytic residues with the DNA substrate might promote the phosphoryl transfer reactions. PMID:20298194

  6. DRAFT LANDSAT DATA MOSAIC: MONTGOMERY COUNTY, TEXAS; HARRIS COUNTY, TEXAS; FORT BEND COUNTY, TEXAS; BRAZORIA COUNTY, TEXAS; GALVESTON COUNTY, TEXAS

    EPA Science Inventory

    This is a draft Landsat Data Mosaic, which contains remote sensing information for Montgomery County, Texas Harris County, Texas Fort Bend County, Texas Brazoria County, Texas Galveston County, and Texas Imagery dates on the following dates: October 6, 1999 and September 29, 200...

  7. Counties with Established Counties without Established

    E-print Network

    Counties with Established Burn Bans Counties without Established Burn Bans Outdoor Burn Bans:http://www.srh.noaa.gov/data/index.php Burn Ban RSS feed available at http://tfsfrp.tamu.edu/wildfires/BurnBan.xml County Burn Ban Orders is not responsible for establishing or removing burning bans. The Texas A&M Forest Service is only displaying

  8. Aberrant Cosmc genes result in Tn antigen expression in human colorectal carcinoma cell line HT-29

    PubMed Central

    Yu, Xiaofeng; Du, Zhenzhen; Sun, Xuhong; Shi, Chuanqin; Zhang, Huaixiang; Hu, Tao

    2015-01-01

    The Tn antigen, which arises from mutation in the Cosmc gene is one of the most common tumor associated carbohydrate antigens. Cosmc resides in X24 encoded by a single gene and functions as a specific molecular chaperone for T-synthase. While the Tn antigen cannot be detected in normal cells, Cosmc mutations inactivate T-synthase and consequently result in Tn antigen expression within certain cancers. In addition to this Cosmc mutation-induced expression, the Tn antigen is also expressed in such cell lines as Jurkat T, LSC and LS174T. Whether the Cosmc mutation is present in the colon cancer cell line HT-29 is still unclear. Here, we isolate HT-29-Tn+ cells from HT-29 cells derived from a female colon cancer patient. These HT-29-Tn+ cells show a loss of the Cosmc gene coding sequence (CDS) leading to an absence of T-synthase activity and Tn antigen expression. Additionally, almost no methylation of Cosmc CpG islands was detected in HT-29-Tn+ as well as in HT-29-Tn- and Tn- tumor cells from male patients. In contrast, the methylation frequency of CpG island of Cosmc in normal female cells was ~50%. Only one active allele of Cosmc existed in HT-29-Tn+ and HT-29-Tn- cells as based upon detection of SNP sites. These results indicate that Tn antigens expression and T-synthase inactivity in HT-29-Tn+ cells can be related to the absence of CDS in Cosmc active alleles, while an inactive allele deletion of Cosmc in HT-29 cells has no influence on Cosmc function.

  9. 76 FR 33805 - Tennessee Disaster Number TN-00052

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ...hereby amended to include the following areas as adversely affected by the disaster. Primary Counties: Fentress, Franklin, Giles, Hickman, Houston, Jackson, Knox, Lawrence, Lewis, Lincoln, Loudon, Marshall, Montgomery, Moore, Perry,...

  10. Dates Days Activity Location Deadline Cost June 9 Sun. Kayaking Harpeth River, TN (KS to GT) 5/31 $33

    E-print Network

    Bordenstein, Seth

    Dates Days Activity Location Deadline Cost June 9 Sun. Kayaking Harpeth River, TN (KS to GT) 5/31 $33 15 Sat. Canoeing * Families welcome * Harpeth River, TN (The Narrows) 6/7 $22 16 Sun. Hike, TN 6/14 $22 21-23 Fri. - Sun. Backpacking Great Smoky Mountain National Park, TN 6/14 $97 30 Sun

  11. Transfer of Tn5385, a Composite, Multiresistance Chromosomal Element from Enterococcus faecalis

    PubMed Central

    Rice, Louis B.; Carias, Lenore L.

    1998-01-01

    Tn5385 is a ca. 65-kb element integrated into the chromosomes of clinical Enterococcus faecalis strains CH19 and CH116. It confers resistance to erythromycin, gentamicin, mercuric chloride, streptomycin, tetracycline-minocycline, and penicillin via ?-lactamase production. Tn5385 is a composite structure containing regions previously found in staphylococcal and enterococcal plasmids. Several transposons and transposon-like elements within Tn5385 have been identified, including conjugative transposon Tn5381, composite transposon Tn5384, and elements indistinguishable from staphylococcal transposons Tn4001 and Tn552. The divergent regions of Tn5385 are linked by a series of insertion sequence (IS) elements (IS256, IS257, and IS1216) of staphylococcal and enterococcal origin. The ends of Tn5385 consist of directly repeated copies of enterococcal IS1216. Within the chromosomes of strains CH19 and CH116, Tn5385 has interrupted an open reading frame with substantial homology to previously described alkyl hydrogen peroxide reductase genes. Segments of this open reading frame in both CH19 and CH116 have been deleted, but the amount of deleted DNA differs for the two insertions. Transfer of Tn5385 from both donors into E. faecalis recipients occurs at a low frequency. Two types of transconjugants have been identified. In one type, the target alkyl hydrogen peroxide reductase open reading frame has been deleted, and sequences flanking Tn5385 in the respective donors are carried over to the transconjugants. These data suggest that the mechanism of Tn5385 insertion into the recipient chromosome in these transconjugants was recombination across flanking regions in the donors and homologous sequences in the recipients. The second type of transconjugant appears to have resulted from excision of Tn5385 from the CH19 chromosome by recombination across the terminal IS1216 elements and insertion into the recipient chromosome by recombination across Tn5381 (within Tn5385) and a previously transferred Tn5381 copy in the recipient chromosome. These data confirm that Tn5385 is a composite structure with genetic material from diverse genera and suggest that it is a functional transposon. They also suggest that chromosomal recombination is a mechanism of genetic exchange in enterococci. PMID:9457879

  12. Tn916 insertional inactivation of multiple genes on the chromosome of Streptococcus mutans GS-5.

    PubMed Central

    Procino, J K; Marri, L; Shockman, G D; Daneo-Moore, L

    1988-01-01

    Streptococcus mutans GS-5 was transformed with the Escherichia coli plasmid pAM150 containing the cloned streptococcal transposon Tn916. Southern blot analyses with the tetracycline-resistant determinant of Tn916 showed that Tn916 was inserted into the chromosome of S. mutans at a variety of different sites. Tn916 insertions resulted in the inactivation of genes that code for various steps in the biosynthesis of several different amino acids. Two auxotrophs which contained a single copy of Tn916 were shown to revert to prototrophy at frequencies of about 10(-8). All of the revertant prototrophs were susceptible to tetracycline, indicating regeneration of the functional gene by excision of Tn916. Images PMID:2844674

  13. Evaluation of endosonography in TN staging of oesophageal cancer.

    PubMed Central

    Ziegler, K; Sanft, C; Zeitz, M; Friedrich, M; Stein, H; Häring, R; Riecken, E O

    1991-01-01

    Strategies for the treatment of cancer of the oesophagus depend on the tumour stage at the time of diagnosis. Resection, the only curative treatment, is confined to early tumour stages. Tumours with local infiltration are usually unresectable and require palliative treatment. Computed tomography has been widely used for preoperative staging but often fails to define this correctly. Endoscopic ultrasound allows direct visualisation of the parietal wall and may be useful in staging gastrointestinal tumours. In a comparative prospective study, 52 patients with tumours of the oesophagus were investigated preoperatively both by endoscopic ultrasound and computed tomography to determine the stage of tumour infiltration and local lymph node involvement. Thirty seven of these patients underwent operation, resection, or dissection and entered the study. The intraoperative findings or the histopathological assessment, or both, were taken as a reference. For all TN stages of oesophageal tumours, correct preoperative staging was accomplished by endoscopic ultrasound in 89% for T stage and 69% for N stage compared with 51% and 51% respectively by computed tomography (highly significant using Fisher's exact test). This study shows that endoscopic ultrasound is useful in preoperative TN staging of tumours of the oesophagus. Images Figure 1 Figure 2 PMID:1991632

  14. Reduced Virulence of Group A Streptococcal Tn916 Mutants That Do Not Produce Streptolysin S

    Microsoft Academic Search

    STEPHEN D. BETSCHEL; SERGIO M. BORGIA; NEIL L. BARG; DONALD E. LOW; JOYCE C. S. DE AZAVEDO

    1998-01-01

    Streptolysin S (SLS) is a potent cytolytic toxin produced by nearly all group A streptococci (GAS). SLS- deficient Tn916 insertional mutants were generated from two clinical isolates of GAS, MGAS166s and T18Ps (M serotypes 1 and 18, respectively), by transposon mutagenesis using Tn916 donor strain Enterococcus faecalis CG110. Representative nonhemolytic transconjugants SBNH5 and SB30-2 each harbored a single Tn916 insertion

  15. Transposon Tn 5 mutagenesis of genes for the photosynthetic apparatus in Rhodopseudomonas capsulata

    Microsoft Academic Search

    Norbert Kaufmann; Hendrik Hüdig; Gerhart Drews

    1984-01-01

    Three plasmids containing the transposon Tn5, i.e. pSUP201::Tn5, pACYC184::Tn5 and pJB4JI were transferred from Escherichia coli to Rhodopseudomonas capsulata in order to mutagenize the genome. Mutants defective in bacteriochlorophyll and carotenoid synthesis and mutants unable to form the photochemical reaction center or one of the light-harvesting complexes were isolated. Of special interest were mutants that could not form the light-harvesting

  16. BWR - Spent Fuel Transport and Storage with the TN{sup TM}9/4 and TN{sup TM}24BH Casks

    SciTech Connect

    Wattez, L. [COGEMA LOGISTICS - AREVA Group (France); Marguerat, Y. [BKW FMB Energy Ltd (Switzerland); Hoesli, C. [ZWILAG Zwischenlager Wuerenlingen AG (Switzerland)

    2006-07-01

    The Swiss Nuclear Utilities have started in 2001 to store spent fuel in dry metallic dual-purpose casks at ZWILAG, the Swiss interim storage facility. BKW FMB Energy Ltd., the Muehleberg Nuclear Power Plant owner, is involved in this process and has elected to store its BWR spent fuel in a new high capacity dual-purpose cask, the TNeTeM24BH from the COGEMA Logistics/TRANSNUCLEAR TN{sup TM}24 family. The Muehleberg BWR spent fuels are transported by road in a medium size shuttle transport cask and then transferred to a heavy transport/storage cask (dry transfer) in the hot cell of ZWILAG site. For that purpose, COGEMA Logistics designed and supplied: - Two shuttle casks, TN{sup TM}9/4, mainly devoted to transport of spent fuel from Muehleberg NPP to ZWILAG. Licensed according to IAEA 1996, the TN{sup TM}9/4 is a 40 ton transport cask, for 7 BWR high bum-up spent fuel assemblies. - A series of new high capacity dual-purpose casks, TN{sup TM}24BH, holding 69 BWR spent fuels. Two transport campaigns took place in 2003 and 2004. For each campaign, ten TN{sup TM}9/4 round trips are performed, and one TN{sup TM}24BH is loaded. 5 additional TN{sup TM}24BH are being manufactured for BKW, and the next transport campaigns are scheduled from 2006. The TN{sup TM}24BH high capacity dual purpose cask and the TN{sup TM}9/4 transport cask characteristics and capabilities will then be detailed. (authors)

  17. Partial Excision of blaKPC from Tn4401 in Carbapenem-Resistant Klebsiella pneumoniae

    PubMed Central

    Chen, Liang; Chavda, Kalyan D.; Mediavilla, José R.; Jacobs, Michael R.; Levi, Michael H.; Bonomo, Robert A.

    2012-01-01

    We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial blaKPC fragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding blaKPC is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic. PMID:22203593

  18. Partial excision of blaKPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae.

    PubMed

    Chen, Liang; Chavda, Kalyan D; Mediavilla, José R; Jacobs, Michael R; Levi, Michael H; Bonomo, Robert A; Kreiswirth, Barry N

    2012-03-01

    We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial bla(KPC) fragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding bla(KPC) is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic. PMID:22203593

  19. Target immunity of the Tn3-family transposon Tn4430 requires specific interactions between the transposase and the terminal inverted repeats of the transposon.

    PubMed

    Nicolas, Emilien; Lambin, Michaël; Hallet, Bernard

    2010-08-01

    Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo and remained responsive to wild-type immunity conferred by the Tn4430 inverted repeat end. Intriguingly, the presence of lacO repeats within the target was found to strongly influence target site selection by Tn4430, but in a LacI-independent manner. PMID:20562304

  20. Regional Lead Agents and County Coordinators 2011 RESPONSIBILITY NAME COUNTY

    E-print Network

    Bolding, M. Chad

    #12;Regional Lead Agents and County Coordinators 2011 RESPONSIBILITY NAME COUNTY REGION 1 Regional Lead Millie Davenport HGIC County Coordinator Matt Burns Pickens County Coordinator Marty Watt Anderson County Coordinator Morris Warner Oconee REGION 2 Regional Lead Danny Howard Greenville County Coordinator

  1. Percent Uninsured by County

    NSDL National Science Digital Library

    ManyEyes

    This is a county by county visualization of the percentage of residents that are uninsured. The data are from a set available here: http://www-958.ibm.com/software/data/cognos/manyeyes/datasets/percent-uninsured-by-county/versions/1

  2. A review of the T_N theory and its cousins

    E-print Network

    Tachikawa, Yuji

    2015-01-01

    The T_N theory is a four-dimensional N=2 superconformal field theory that has played a central role in the analysis of supersymmetric dualities in the last few years. The aim of this review is to collect known properties of the T_N theory and its cousins in one place as a quick reference.

  3. A review of the T_N theory and its cousins

    E-print Network

    Yuji Tachikawa

    2015-04-07

    The T_N theory is a four-dimensional N=2 superconformal field theory that has played a central role in the analysis of supersymmetric dualities in the last few years. The aim of this review is to collect known properties of the T_N theory and its cousins in one place as a quick reference.

  4. Geological and petrophysical characterization of the Ferron Sandstone for 3-D simulation of a fluvial-deltaic reservoir. Deliverable 1.4.4: Ferron Sandstone lithofacies and case-study areas, Emery and Sevier Counties, Utah

    SciTech Connect

    Allison, M.L.

    1996-01-04

    The types of dominantly sandstone lithofacies that characterize the Cretaceous Ferron Sandstone reservoir types were tentatively identified before the project began. These reservoir types were defined and mapped at the regional scale and are the subject of the detailed, highly focused case studies. The purpose of conducting detailed case-study analysis is to develop a comprehensive, interdisciplinary, and quantitative characterization of fluvial-deltaic reservoirs which will allow realistic inter-well and reservoir-scale modeling to be used for improved oil-field development in actual reservoirs world-wide. The resulting benefits and value may: (1) increase recoverable reserves by identifying untapped compartments created by reservoir heterogeneity, (2) reduce development costs by more efficiently siting infill drilling locations, (3) increase deliverability by exploiting the reservoir along optimal fluid-flow paths, (4) enhance the application of new technologies, such as horizontal drilling, by identifying optimal drilling directions to maximize fluid-flow, and (5) identify reservoir trends for field extension drilling. Various geologic studies of the Ferron Sandstone were reviewed to compile a list of locations and types of lithofacies in the Ferron Sandstone to be examined in greater detail as part of the subsequent case studies. Preliminary regional interpretations were also used to help select the type and location of lithofacies for case studies. Potential case-study sites were delineated during several reconnaissance field trips by the geologic team. Two case-study sites were selected for the project: Ivie Creek and Willow Springs Wash, in the central and southern parts respectively of the project study area. Results are discussed.

  5. 77 FR 13073 - Designation for the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA Areas

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ...Lincoln, NE; Memphis, TN; and Sioux City, IA Areas AGENCY: Grain Inspection, Packers...Lincoln, NE; Memphis, TN; and Sioux City, IA areas, Lincoln, Midsouth, and Sioux City...Sioux City, IA......... (712) 255-8073...

  6. 75 FR 33765 - Foreign-Trade Zone 77-Memphis, TN, Application for Subzone, Delta Faucet Company (Faucets...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-15

    ...Zone 77--Memphis, TN, Application for Subzone, Delta Faucet Company (Faucets); Jackson, TN An application...subzone status for the faucet manufacturing facility of Delta Faucet Company (Delta), located in Jackson, Tennessee. The...

  7. Tn6188 - a novel transposon in Listeria monocytogenes responsible for tolerance to benzalkonium chloride.

    PubMed

    Müller, Anneliese; Rychli, Kathrin; Muhterem-Uyar, Meryem; Zaiser, Andreas; Stessl, Beatrix; Guinane, Caitriona M; Cotter, Paul D; Wagner, Martin; Schmitz-Esser, Stephan

    2013-01-01

    Controlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S.) aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC) as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR) transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs) (28.5 ± 4.7 mg/l) than strains without Tn6188 (14 ± 3.2 mg/l). Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that ?qacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L. monocytogenes strains. PMID:24098567

  8. International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21.

    PubMed

    Novais, Angela; Baquero, Fernando; Machado, Elisabete; Cantón, Rafael; Peixe, Luísa; Coque, Teresa M

    2010-02-01

    TEM-24 remains one of the most widespread TEM-type extended-spectrum beta-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-DeltaTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly penetrating clones and highly promiscuous plasmids in the spread of antibiotic resistance, and it contributes to the elucidation of the origin and evolution of TEM-2 ESBL derivatives. PMID:19995930

  9. 77 FR 21505 - Proposed Amendment of Class D and E Airspace; Blountville, TN, and Revocation of Class E Airspace...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-10

    ...11-ASO-28] Proposed Amendment of Class D and E Airspace; Blountville, TN, and Revocation of Class E Airspace; Tri-City, TN AGENCY: Federal Aviation...This action proposes to amend Class D and Class E surface airspace at Blountville, TN, and...

  10. Synapsis and catalysis by activated Tn3 resolvase mutants.

    PubMed

    Olorunniji, Femi J; He, Jiuya; Wenwieser, Sandra V C T; Boocock, Martin R; Stark, W Marshall

    2008-12-01

    The serine recombinase Tn3 resolvase catalyses recombination between two 114 bp res sites, each of which contains binding sites for three resolvase dimers. We have analysed the in vitro properties of resolvase variants with 'activating' mutations, which can catalyse recombination at binding site I of res when the rest of res is absent. Site I x site I recombination promoted by these variants can be as fast as res x res recombination promoted by wild-type resolvase. Activated variants have reduced topological selectivity and no longer require the 2-3' interface between subunits that is essential for wild-type resolvase-mediated recombination. They also promote formation of a stable synapse comprising a resolvase tetramer and two copies of site I. Cleavage of the DNA strands by the activated mutants is slow relative to the rate of synapsis. Stable resolvase tetramers were not detected in the absence of DNA or bound to a single site I. Our results lead us to conclude that the synapse is assembled by sequential binding of resolvase monomers to site I followed by interaction of two site I-dimer complexes. We discuss the implications of our results for the mechanisms of synapsis and regulation in recombination by wild-type resolvase. PMID:19015124

  11. Yeast plasmid 2-micron circle promotes recombination within bacterial transposon Tn5.

    PubMed Central

    Jayaram, M; Broach, J R

    1983-01-01

    The site-specific recombination system (FLP) encoded by the yeast plasmid 2-micron circle can also act in yeast on the inverted repeats of the bacterial transposon Tn5. The efficiency of this recombination is dependent on the location of Tn5 within the 2-micron circle genome but can be as high as that observed for 2-micron circle itself. Comparison of the DNA sequences between the Tn5 repeat and the 2-micron circle recombination region reveals certain strikingly similar structural features that might be important in the recombination reaction. Images PMID:6316350

  12. Multicopy integration of mini-Tn7 transposons into selected chromosomal sites of a Salmonella vaccine strain.

    PubMed

    Roos, Karen; Werner, Esther; Loessner, Holger

    2015-01-01

    Chromosomal integration of expression modules for transgenes is an important aspect for the development of novel Salmonella vectors. Mini-Tn7 transposons have been used for the insertion of one such module into the chromosomal site attTn7, present only once in most Gram-negative bacteria. However, integration of multiple mini-Tn7 copies might be suitable for expression of appropriate amounts of antigen or combination of different modules. Here we demonstrate that integration of a 9.6?kb mini-Tn7 harbouring the luciferase luxCDABE (lux) occurs at the natural attTn7 site and simultaneously other locations of the Salmonella chromosome, which were engineered using ?-Red recombinase to contain one or two additional artificial attTn7 sites (a-attTn7). Multicopy integration even at closely spaced attTn7 sites was unexpected in light of the previously reported distance-dependent Tn7 target immunity. Integration of multiple copies of a mini-Tn7 containing a gfp cassette resulted in increasing green fluorescence of bacteria. Stable consecutive integration of two mini-Tn7 encoding lacZ and lux was achieved by initial transposition of lacZ-mini-Tn7, subsequent chromosomal insertion of a-attTn7 and a second round of transposition with lux-mini-Tn7. Mini-Tn7 thus constitutes a versatile method for multicopy integration of expression cassettes into the chromosome of Salmonella and possibly other bacteria. PMID:25488129

  13. Inhibition of fungi and gram-negative bacteria by bacteriocin BacTN635 produced by Lactobacillus plantarum sp. TN635.

    PubMed

    Smaoui, Slim; Elleuch, Lobna; Bejar, Wacim; Karray-Rebai, Ines; Ayadi, Imen; Jaouadi, Bassem; Mathieu, Florence; Chouayekh, Hichem; Bejar, Samir; Mellouli, Lotfi

    2010-10-01

    The aim of this study was to evaluate 54 lactic acid bacteria (LAB) strains isolated from meat, fermented vegetables and dairy products for their capacity to produce antimicrobial activities against several bacteria and fungi. The strain designed TN635 has been selected for advanced studies. The supernatant culture of this strain inhibits the growth of all tested pathogenic including the four Gram-negative bacteria (Salmonella enterica ATCC43972, Pseudomonas aeruginosa ATCC 49189, Hafnia sp. and Serratia sp.) and the pathogenic fungus Candida tropicalis R2 CIP203. Based on the nucleotide sequence of the 16S rRNA gene of the strain TN635 (1,540 pb accession no FN252881) and the phylogenetic analysis, we propose the assignment of our new isolate bacterium as Lactobacillus plantarum sp. TN635 strain. Its antimicrobial compound was determined as a proteinaceous substance, stable to heat and to treatment with surfactants and organic solvents. Highest antimicrobial activity was found between pH 3 and 11 with an optimum at pH = 7. The BacTN635 was purified to homogeneity by a four-step protocol involving ammonium sulfate precipitation, centrifugal microconcentrators with a 10-kDa membrane cutoff, gel filtration Sephadex G-25, and C18 reverse-phase HPLC. SDS-PAGE analysis of the purified BacTN635, revealed a single band with an estimated molecular mass of approximately 4 kDa. The maximum bacteriocin production (5,000 AU/ml) was recorded after a 16-h incubation in Man, Rogosa, and Sharpe (MRS) medium at 30 degrees C. The mode of action of the partial purified BacTN635 was identified as bactericidal against Listeria ivanovii BUG 496 and as fungistatic against C. tropicalis R2 CIP203. PMID:19888697

  14. Continuing Education for County Officials The duties and responsibilities of county government

    E-print Network

    Continuing Education for County Officials The duties and responsibilities of county government, the V.G. Young Institute of County Government provides continuing education programs for local government officials, including county judges and commissioners, county treasurers, county and district

  15. 75 FR 28303 - Setco Automotive, Inc., Paris, TN; Notice of Revised Determination on Reconsideration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-20

    ...Administration [TA-W-71,401] Setco Automotive, Inc., Paris, TN; Notice of Revised...competitive with articles into which the automotive clutch products produced by the workers...subject firm sold component parts (automotive clutch products) to be...

  16. Submarine Ring of Fire 2003 Mariana Arc R/V T. G. Thompson Cruise TN-153

    E-print Network

    #12;i Submarine Ring of Fire 2003 ­ Mariana Arc R/V T. G. Thompson Cruise TN-153 February 9 - March: Susan Merle, Robert Embley, Edward Baker and Bill Chadwick TABLE OF CONTENTS 1.0 Submarine Ring of Fire

  17. 77 FR 59994 - Nuclear Fuel Services, Inc., Erwin, TN; Issuance of License Renewal

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-01

    ...Inc., Erwin, TN; Issuance of License Renewal AGENCY: U.S. Nuclear Regulatory Commission...ACTION: Notice of issuance of license renewal...providing notice of the issuance of License Renewal to Material License No. SNM-124,...

  18. Approved for public release; distribution is unlimited. ERDC/TN SAV-11-1

    E-print Network

    US Army Corps of Engineers

    the seed reproductive potential of two species of submersed aquatic angiosperms, Ruppia maritima (widgeon-bearing plant stems. #12;ERDC/TN SAV-11-1 May 2011 2 seed-bearing stems of Ruppia maritima (widgeon grass

  19. 75 FR 28302 - American Food and Vending Spring Hill, TN; Notice of Negative Determination Regarding Application...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-20

    ...Administration [TA-W-72,606] American Food and Vending Spring Hill, TN...workers and former workers at American Food and Vending, Spring Hill, Tennessee...previously-submitted information from American Food and Vending revealed no...

  20. Construction of Mini-Tn 4001tet and Its Use in Mycoplasma gallisepticum

    Microsoft Academic Search

    Ina Pour-El; Cary Adams; F. Chris Minion

    2002-01-01

    The Mollicutes are a group of cell-wall-less bacteria and are important plant and animal pathogens. Progress toward analyzing their pathogenic mechanisms has been hampered by the few available genetic tools. Of the two transposons shown to function in mycoplasmas, only Tn4001 is readily amenable to modification and development. One disadvantage of using Tn4001 in mycoplasmas has been independent insertion of

  1. Analysis of a heterogeneous group of human breast carcinoma associated glycoproteins bearing the Tn determinant

    Microsoft Academic Search

    Eduardo Osinaga; Gianfranco Pancino; Nicole Porchet; Nora Berois; Patricia De Cremoux; Dominique Mistro; Jean Pierre Aubert; Fabian Calvo; Alberto Roseto

    1994-01-01

    The Tn determinant (GalNAca-O-Ser\\/Thr) is expressed by about 90% of human carcinomas, but is cryptic in most normal human tissues. A murine monoclonal antibody (MAb) 83D4, developed following immunization with human breast carcinoma cells, reacts with a Tn-related epitope. In the present study we characterized the glycoprotein antigen identified by 83D4 in the human breast carcinoma cell line MCF-7. We

  2. Transposon Tn 5 mutagenesis in Azospirillum lipoferum : isolation of indole acetic acid mutants

    Microsoft Academic Search

    Mohammed Safwat Abdel-Salam; Walter Klingmiiller

    1987-01-01

    A method for transposon mutagenesis in Azospirillum lipoferum 29708 is reported with transposon Tn5. The suicide plasmid pSUP2021 was used to deliver Tn5 in A. lipoferum using Escherichia coli SM10 as the donor. Neomycin-resistant transconjugants were detected at a frequency of 6x10-6 per recipient. Different types of mutants were isolated, e.g. auxotrophic, coloured, IAA-negative, and IAA-overproducers. Among the auxotrophic mutants,

  3. Directed transposon Tn 5 mutagenesis and complementation in slow-growing, broad host range cowpea Rhizobium

    Microsoft Academic Search

    Mittur N. Jagadish; Aladar A. Szalay

    1984-01-01

    Site-directed Tn5 mutagenesis by gene replacement method, via homologous recombination, was used to identify symbiotically essential regions in the genome of cowpea Rhizobium spp. IRc78. Transposon insertions with-in the nifK hybridizing region or in the regions spanning 10 kb downstream of the nifK have revealed the presence of functional genes required for nitrogen fixation. Six single Tn5 insertions resulted in

  4. Mini-Tn 7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii

    Microsoft Academic Search

    Ayush Kumar; Courtney Dalton; Jenny Cortez-Cordova; Herbert P. Schweizer

    2010-01-01

    Acinetobacter baumannii is an emerging pathogen that causes serious infections with high mortality rates in immunocompromised individuals. Genetic manipulations in this medically-relevant pathogen are limited by the paucity of molecular tools. In this study, we show the application of the mini-Tn7-based single copy insertion system in A. baumannii. Mini-Tn7 elements are known to integrate at a naturally evolved, therefore presumably

  5. Biochemical and functional characterization of the Tn-specific lectin from Salvia sclarea seeds.

    PubMed

    Medeiros, A; Bianchi, S; Calvete, J J; Balter, H; Bay, S; Robles, A; Cantacuzène, D; Nimtz, M; Alzari, P M; Osinaga, E

    2000-03-01

    SSL, the lectin isolated from Salvia sclarea seeds, recognizes the Tn antigen (GalNAcalpha-O-Ser/Thr), a specific marker of many human carcinomas. Two-dimensional electrophoresis, amino-acid and amino-sugar analysis, and MALDI-TOF MS showed that SSL is an acidic (pI 5.5), 60-61-kDa dimeric glycoprotein composed of apparently identical subunits linked by a single disulfide bond. The apparent molecular mass of SSL in solution determined by equilibrium sedimentation analytical ultracentrifugation was 59 +/- 9 kDa. This value did not change in the pH range 2.5-8.5, indicating that SSL does not associate into higher order structures. Tandem mass spectrometry and methylation analysis of N-glycans released from SSL by hydrazinolysis indicated that SSL possesses 2-3 glycosylation sites occupied with the typical plant glycans Manalpha1-6[(Manalpha1-3)(Xylbeta1-2)]Manbeta1-4 -GlcNAcbeta1-4(Fucalp ha1-3)GlcNAc and [(Manalpha1-3/6)(Xylbeta1-2)]Manbeta1-4-GlcNAcbeta1 -4(Fucalpha1-3)Glc NAc. The influence of adjacent Tn structures on the binding of two Tn-specific lectins (SSL and the isolectin B4 from Vicia villosa) and an anti-Tn monoclonal antibody (mAb 83D4) was evaluated using synthetic Tn glycopeptides. The binding of both lectins to the synthetic Tn glycopeptides was independent of the density of Tn structures. On the other hand, mAb 83D4 only reacted with glycopeptides displaying two or three consecutive Tn structures. PMID:10691981

  6. Immunoreactive T and Tn epitopes in cancer diagnosis, prognosis, and immunotherapy

    Microsoft Academic Search

    Georg F. Springer

    1997-01-01

    Aberrant glycosylation is a hallmark of cancer cells. The blood group precursors T (Thomsen-Friedenreich) and Tn epitopes\\u000a are shielded in healthy and benign-diseased tissues but uncovered in approx. 90% of carcinomas. T and Tn glycoproteins are\\u000a specific, autoimmunogenic pancarcinoma antigens. These antigens may also be found in neoplastic blood cells (and on LTV-II\\u000a infected T lymphocytes). Fundamental chemical and physical

  7. A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2

    PubMed Central

    Gill, Sukhvinder; Krupovic, Mart; Desnoues, Nicole; Béguin, Pierre; Sezonov, Guennadi; Forterre, Patrick

    2014-01-01

    We report the characterization of a DNA primase/polymerase protein (PolpTN2) encoded by the pTN2 plasmid from Thermococcus nautilus. Sequence analysis revealed that this protein corresponds to a fusion between an N-terminal domain homologous to the small catalytic subunit PriS of heterodimeric archaeal and eukaryotic primases (AEP) and a C-terminal domain related to their large regulatory subunit PriL. This unique domain configuration is not found in other virus- and plasmid-encoded primases in which PriS-like domains are typically fused to different types of helicases. PolpTN2 exhibited primase, polymerase and nucleotidyl transferase activities and specifically incorporates dNTPs, to the exclusion of rNTPs. PolpTN2 could efficiently prime DNA synthesis by the T. nautilus PolB DNA polymerase, suggesting that it is used in vivo as a primase for pTN2 plasmid replication. The N-terminal PriS-like domain of PolpTN2 exhibited all activities of the full-length enzyme but was much less efficient in priming cellular DNA polymerases. Surprisingly, the N-terminal domain possesses reverse transcriptase activity. We speculate that this activity could reflect an ancestral function of AEP proteins in the transition from the RNA to the DNA world. PMID:24445805

  8. Enterococcal transposon Tn5384: evolution of a composite transposon through cointegration of enterococcal and staphylococcal plasmids.

    PubMed

    Bonafede, M E; Carias, L L; Rice, L B

    1997-09-01

    Mechanisms for the possible transfer of antimicrobial resistance genes between staphylococci and enterococci remain poorly defined. We have previously reported the transfer between Enterococcus faecalis strains of a multiresistance chromosomal element (beta-lactamase positive and resistance to erythromycin, gentamicin, mercuric chloride, streptomycin, and tetracycline) which we have tentatively designated Tn5385. Tn5385 is a composite of several smaller transposable elements, including Tn5384, a 26-kb composite transposon conferring resistance to erythromycin, gentamicin, and mercuric chloride. Analyses of 7 kb within Tn5384 and flanking sequences within the larger element revealed sequences characteristic of staphylococcal beta-lactamase and small, mobilizable plasmids flanking a region with a sequence identical to those of the replication genes previously described for enterococcal and streptococcal broad-host-range plasmids. These diverse regions are linked by insertion sequences IS256 and IS257 in a manner which suggests a series of cointegration events as the genesis of the current relationship. Taken together, these data suggest that Tn5384 and the larger element within which it is incorporated (Tn5385) evolved at least in part as a result of cointegration between an enterococcal broad-host-range plasmid and staphylococcal beta-lactamase and small mobilizable plasmids. These results implicate broad-host-range plasmids in the transfer of resistance determinants from staphylococci to enterococci. PMID:9303373

  9. Enterococcal transposon Tn5384: evolution of a composite transposon through cointegration of enterococcal and staphylococcal plasmids.

    PubMed Central

    Bonafede, M E; Carias, L L; Rice, L B

    1997-01-01

    Mechanisms for the possible transfer of antimicrobial resistance genes between staphylococci and enterococci remain poorly defined. We have previously reported the transfer between Enterococcus faecalis strains of a multiresistance chromosomal element (beta-lactamase positive and resistance to erythromycin, gentamicin, mercuric chloride, streptomycin, and tetracycline) which we have tentatively designated Tn5385. Tn5385 is a composite of several smaller transposable elements, including Tn5384, a 26-kb composite transposon conferring resistance to erythromycin, gentamicin, and mercuric chloride. Analyses of 7 kb within Tn5384 and flanking sequences within the larger element revealed sequences characteristic of staphylococcal beta-lactamase and small, mobilizable plasmids flanking a region with a sequence identical to those of the replication genes previously described for enterococcal and streptococcal broad-host-range plasmids. These diverse regions are linked by insertion sequences IS256 and IS257 in a manner which suggests a series of cointegration events as the genesis of the current relationship. Taken together, these data suggest that Tn5384 and the larger element within which it is incorporated (Tn5385) evolved at least in part as a result of cointegration between an enterococcal broad-host-range plasmid and staphylococcal beta-lactamase and small mobilizable plasmids. These results implicate broad-host-range plasmids in the transfer of resistance determinants from staphylococci to enterococci. PMID:9303373

  10. Tn6249, a new Tn6162 transposon derivative carrying a double-integron platform and involved with acquisition of the blaVIM-1 metallo-?-lactamase gene in Pseudomonas aeruginosa.

    PubMed

    Di Pilato, Vincenzo; Pollini, Simona; Rossolini, Gian Maria

    2015-03-01

    The In70.2 integron platform appears to be a conserved structure involved in the dissemination of the blaVIM-1 metallo-?-lactamase gene in Pseudomonas aeruginosa. The genetic context of the In70.2 integron platform from P. aeruginosa VR-143/97, the VIM-1-producing index strain isolated in Italy in 1997, was fully characterized by a next-generation sequencing approach refined by conventional sequencing. The In70.2 integron platform from VR-143/97 was found to be associated with a defective Tn402-like transposon inserted into the urf2 gene of a Tn3 family transposon of an original structure, named Tn6249, which also carried a partially deleted mer operon and an In90 integron platform in a tail-to-tail orientation. Tn6249 was inserted into a PACS171b-like genomic island, which was in turn inserted into the endA gene of the Pseudomonas chromosomal backbone. Tn6249 showed a similar structure and a conserved location with respect to that of Tn6060, a Tn3 family transposon associated with In70.2 and carrying a double-integron platform, which was detected in a VIM-1-producing P. aeruginosa strain isolated in Australia in 2008. Both Tn6249 and Tn6060 are apparently derived from Tn6162, a mercury resistance transposon carrying an integron platform, which was found in P. aeruginosa isolates from different geographic locations. The conservation of the genetic context of Tn6249 and Tn6060 suggests an in situ evolution of these elements after the insertion of a Tn6162-like ancestor into the PACS171b-like genomic island (GI) present in the genome of a successful widespread P. aeruginosa clonal lineage. PMID:25547348

  11. County by County in Ohio Genealogy.

    ERIC Educational Resources Information Center

    Khouw, Petta; And Others

    This comprehensive sourcebook and bibliography lists Ohio genealogical materials available at the State Library of Ohio for each county in the state. It serves as a guide to Ohio genealogical resources, including 14,000 volumes and 15,000 microforms in the non-circulating genealogy collection. The library's genealogy collection consists of a broad…

  12. Tn5045, a novel integron-containing antibiotic and chromate resistance transposon isolated from a permafrost bacterium.

    PubMed

    Petrova, Mayya; Gorlenko, Zhosephine; Mindlin, Sofia

    2011-04-01

    A novel antibiotic and chromate resistance transposon, Tn5045, was isolated from a permafrost strain of Pseudomonas sp. Tn5045 is a compound transposon composed of three distinct genetic elements. The backbone element is a Tn1013-like Tn3 family transposon, termed Tn1013?, that contains the tnpA and the tnpR genes, encoding the transposase and resolvase, respectively, the res-site and four genes (orfA, B, C, D) related to different house-keeping genes. The second element is class 1 integron, termed InC?, which is inserted into the Tn1013? res-region and contains 5'-CS-located integrase, 3'-CS-located qacE?1 and sulfonamide resistance sulI genes, and a single cassette encoding the streptomycin resistance aadA2-gene. The third element is a TnOtChr-like Tn3 family transposon termed TnOtChr?, which is inserted into the transposition module of the integron and contains genes of chromate resistance (chrB, A, C, F). Tn5045 is the first example of an ancient integron-containing mobile element and also the first characterized compound transposon coding for both antibiotic and chromate, resistance. Our data demonstrate that antibiotic and chromate resistance genes were distributed in environmental bacteria independently of human activities and provide important insights into the origin and evolution of antibiotic resistance integrons. PMID:21262357

  13. Mucin-type O-glycosylation in Fasciola hepatica: characterisation of carcinoma-associated Tn and sialyl-Tn antigens and evaluation of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase activity

    Microsoft Academic Search

    Teresa Freire; Cecilia Casaravilla; Carlos Carmona; Eduardo Osinaga

    2003-01-01

    Simple mucin-type cancer-associated O-glycan structures, such as the Tn antigen (GalNAc-O-Ser\\/Thr), are expressed by certain helminth parasites. These antigens are involved in several types of receptor–ligand interactions, and they are potential targets for immunotherapy. The aim of this work was to study the initiation pathway of mucin-type O-glycosylation in Fasciolahepatica, performing a biochemical and immunohistochemical characterisation of Tn and sialyl-Tn

  14. Nucleotide Sequence within Tn 3926 Confirms This as a Tn 21-like Transposable Element and Provides Evidence for the Origin of the mer Operon Carried by Plasmid pKLH2

    Microsoft Academic Search

    S. E. V. Osbourn; A. K. Turner; J. Grinsted

    1995-01-01

    The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21, supporting the idea that this insert transposed into a progenitor

  15. Tn5 transposase and tagmentation procedures for massively scaled sequencing projects

    PubMed Central

    Picelli, Simone; Björklund, Åsa K.; Reinius, Björn; Sagasser, Sven; Winberg, Gösta

    2014-01-01

    Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications. PMID:25079858

  16. Genetic analysis of Tn916-like elements conferring tetracycline resistance in clinical isolates of Clostridium difficile.

    PubMed

    Dong, Danfeng; Chen, Xu; Jiang, Cen; Zhang, Lihua; Cai, Gang; Han, Lizhong; Wang, Xuefeng; Mao, Enqiang; Peng, Yibing

    2014-01-01

    As an important clinically relevant pathogen, Clostridium difficile has a high multidrug resistance rate. Conjugative transposons play a vital role in its resistance phenotype. In the present study, 34 tetracycline-resistant clinical isolates of C. difficile were studied to detect tetracycline resistance genes and the presence of transposons. Thirty-two isolates were found to harbour Tn916-like elements carrying the tet(M) resistance gene, of which only one copy existed in the genome by Southern blot analysis. To characterise the genetic organisation of the Tn916-like elements, overlap PCR assays were performed with nine primer pairs, revealing three types of elements designated T1 to T3. The prevalent element T1 lacking PCRA (ORF23 to ORF21) and PCRB (ORF21 to ORF20) products, present in the epidemic ST37 clone, was further analysed by genome walking PCR in the left and right end sequences of the novel Tn916-like element. A gene coding for an FtsK/SpoIIIE family protein was found to replace the ORF24 to ORF21 region in Tn916. Moreover, the element could hardly conjugate between cells by filter mating experiments. These findings suggest that the dissemination of Tn916-like elements in epidemic ST37 strains in China was likely to have been conferred by clonal spread, signifying the importance of future surveillance and characterisation of conjugative transposons. PMID:24176599

  17. Insertion of Transposon Tn917 Derivatives into the Lactococcus lactis subsp. lactis Chromosome

    PubMed Central

    Israelsen, Hans; Hansen, Egon Bech

    1993-01-01

    Two transposition vectors, pTV32 and pLTV1, containing transposon Tn917 derivatives TV32 and LTV1, respectively, were introduced into Lactococcus lactis subsp. lactis MG1614. It was found that pTV32 and pLTV1 replicate and that TV32 and LTV1 transpose in this strain. A protocol for production of a collection of Tn917 insertions in L. lactis subsp. lactis was developed. The physical locations of TV32 on the chromosomal SmaI fragments of 62 independent transpositions were established by pulsed-field gel electrophoresis. These transpositions could be divided into at least 38 different groups that exhibited no Tn917-dominating hot spots on the L. lactis subsp. lactis chromosome. A total of 10 of the 62 transpositions resulted in strains that express ?-galactosidase. This indicates that there was fusion of the promoterless lacZ of the Tn917 derivatives to a chromosomal promoter. Thus, the Tn917-derived transposons should be powerful genetic tools for studying L. lactis subsp. lactis. Images PMID:16348845

  18. Cointegrate resolution following transposition of Tn1792 in Streptomyces avermitilis facilitates analysis of transposon-tagged genes.

    PubMed

    Pitman, Andrew; Herron, Paul; Dyson, Paul

    2002-03-01

    The insertion sequence IS6100, belonging to the IS6 family, normally forms a cointegrate as an end product of transposition. The IS6100-based minitransposon, Tn1792, has been developed as a genetic tool to mutagenise antibiotic-producing Streptomyces. Here, we describe resolution of Tn1792 cointegrates in Streptomyces avermitilis that can facilitate both the initial isolation of Tn1792 insertion mutants and also the subsequent rescue of Tn1792-tagged sequences. This is the first reported example of cointegrate resolution for an IS6-type transposable element. As a result of mutagenesis, several putative genes involved in morphological development and antibiotic production have been isolated. PMID:11777586

  19. In-site interaction evaluation of Tn density by inhibition/competition assays.

    PubMed

    Robles, Ana; Medeiros, Andrea; Berois, Nora; Balter, Henia S; Pauwels, Ernest K; Osinaga, Eduardo

    2010-05-01

    The tumor-associated structure N-acetyl-galactosamine-O-Ser/Thr (Tn antigen), which is overexpressed in various tumor cell types, notably of the breast, ovary and colon, is an interesting determinant that is useful for cancer diagnosis and follow-up. The aim of this research was to study different assay strategies in order to determine the most sensitive system for further application in epitope characterization and binding assessment. The tetrameric isolectin obtained from Vicia villosa seeds (VVLB(4)) shows high affinity for the tumor-associated structure. A monoclonal antibody against VVLB(4), MabVV(34), was generated, and the interaction between MabVV(34) and VVLB(4) was studied by means of binding and inhibition assays. Several synthetic peptides (10 amino acid sequences) designed from the amino acid sequence of VVLB(4) and obtained from trypsin digestion were tested to determine which amino acids were involved in the interaction between MabVV(34) and VVLB(4). The further unraveling of this epitope was investigated by inhibition using designed synthetic peptides as well as mixtures mimicking variable density effect. Under the experimental circumstances, MabVV(34) was able to inhibit the binding of VVLB(4) to Tn. Two of the four peptide sequences assayed showed better inhibition properties. Finally, mixtures containing these selected sequences allowed the evaluation of binding and inhibition as a function of Tn density. We conclude that the present study facilitates the further development of a specific Tn marker and may contribute to the development of Tn-like radiolabelled peptides or Tn-specific radiolabelled fragments providing a highly selective tool for cancer diagnosis and treatment. This strategy may contribute to characterize the new generation of radiopharmaceuticals for diagnosis and therapy based on biomolecules like antibodies, fragments or peptides, whose application is directly guided by their specific molecular recognition. PMID:20447557

  20. Persistent Tn polyagglutination syndrome during febrile neutropenia: a case report and review of the literature

    PubMed Central

    2011-01-01

    Introduction Tn polyagglutination syndrome is a rare disorder that has been reported on only a few occasions in the literature, and, to the best of our knowledge, never before in the context of febrile neutropenia. Case presentation We report the case of a 26-year-old Caucasian woman who presented to our emergency department complaining of a persistent fever over the previous three days. She had a history of long-standing refractory pancytopenia with multi-lineage dysplasia and severe neutropenia, but she had rarely experienced infection. The results of a physical examination and multiple laboratory tests were unremarkable. While investigating the possible causes of the refractory, long-standing pancytopenia, the possibility of a polyagglutinable state was suggested. Blood samples were sent to the laboratory for an analysis of mixed-field seed lectin agglutination assay. A serum lectin panel confirmed the final diagnosis of Tn-activation. Conclusions We should include Tn-activation in our differential whenever we encounter cases of refractory long-standing idiopathic cytopenias and inconclusive bone marrow results displaying multi-lineage dysplasia. Novel genetic techniques have recently revealed the interesting pathophysiology of this phenomenon. The recognition and inclusion of Tn polyagglutination syndrome in our differential diagnoses has important clinical implications, given its main associated features, such as severe thrombocytopenia and neutropenia, which are usually linked to a benign clinical course and prognosis. Increased awareness of the polyagglutinable disorders will potentially decrease the need for invasive and costly medical interventions and also raises the need for monitoring of this specific sub-set of patients. In addition, the study of the expression and implications of Tn, and other similar antigens, offers a fascinating perspective for the study of its role in the diagnosis, prognosis and immunotherapy of solid tumors and hematological malignancies. The infrequency with which Tn polyagglutination syndrome is encountered, its clinical features and its pathophysiology make it a formidable diagnostic challenge. PMID:21235740

  1. 75 FR 24944 - Adequacy Status of the Milwaukee-Racine, Door County, Manitowoc County, and Sheboygan County...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-06

    ...Manitowoc County, and Sheboygan County, Wisconsin Areas Submitted 8-Hour Ozone Redesignation...Manitowoc County, and Sheboygan County, Wisconsin ozone nonattainment areas are adequate...transportation conformity determinations. Wisconsin submitted a redesignation request...

  2. Synergistic template-free synthesis of dsDNA by Thermococcus nautili primase PolpTN2, DNA polymerase PolB, and pTN2 helicase.

    PubMed

    Béguin, Pierre; Gill, Sukhvinder; Charpin, Nicole; Forterre, Patrick

    2015-01-01

    A combination of three enzymes from the hyperthermophilic archaeon Thermococcus nautili, DNA primase PolpTN2, DNA polymerase PolB, and pTN2 DNA helicase, was found to synthesize up to 300-400 ng/µl dsDNA from deoxynucleotide triphosphates in less than 30 min in the absence of added template DNA and oligonucleotide primer. The reaction did not occur below 64 °C. No synthesis was observed if PolpTN2 or PolB were left out; helicase was not essential but accelerated the reaction. The DNA synthesized consisted of highly reiterated palindromic sequences reaching up to more that 10 kb. Sequence analysis of three independent reaction products synthesized at different temperatures showed that the palindromes shared a common pentanucleotide core, suggesting that random nucleic acid fragments were not responsible for priming the reaction. When enzymes were added sequentially, preincubation with primase plus helicase followed by PolB led to a shorter delay before the onset of the reaction as compared to preincubation with PolB plus helicase followed by primase. This suggests that the primase generates seeds that are subsequently amplified and elongated in synergy with PolB by a mechanism involving hairpin formation and slippage synthesis. PMID:25420601

  3. Tn125-Related Acquisition of blaNDM-Like Genes in Acinetobacter baumannii

    PubMed Central

    Poirel, Laurent; Bonnin, Rémy A.; Boulanger, Anne; Schrenzel, Jacques; Kaase, Martin

    2012-01-01

    A multidrug-resistant Acinetobacter baumannii isolate recovered from a patient hospitalized in Switzerland after a transfer from Serbia produced the NDM-1 carbapenemase. The blaNDM-1 gene was part of a chromosomally located Tn125 composite transposon bracketed by two copies of the same insertion sequence, ISAba125. This transposon was also associated with the acquisition and expression of the blaNDM-2 gene in an A. baumannii isolate in Germany. Tn125 appears to be the main vehicle for dissemination of blaNDM genes in that species. PMID:22143526

  4. Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome.

    PubMed Central

    Shoemaker, N B; Getty, C; Gardner, J F; Salyers, A A

    1986-01-01

    The gene for resistance to erythromycin and clindamycin, which is carried on the conjugative Bacteroides plasmid, pBF4, has been shown previously to be part of an element (Tn4351) that transposes in Escherichia coli. We have now introduced Tn4351 into Bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range IncP plasmid R751 (R751::Tn4351) and (ii) pSS-2, a chimeric plasmid which contains 33 kilobases of pBF4 (including Tn4351) cloned into the IncQ plasmid RSF1010 and which is mobilized by R751. When E. coli HB101, carrying either R751::Tn4351 or R751 and pSS-2, was mated with B. uniformis under aerobic conditions, Emr transconjugants were detected at a frequency of 10(-6) to 10(-5) (R751::Tn4351) or 10(-8) to 10(-6) (R751 and pSS-2). In matings involving pSS-2, all Emr transconjugants contained simple insertions of Tn4351 in the chromosome, whereas in matings involving R751::Tn4351, about half of the Emr transconjugants had R751 cointegrated with Tn4351 in the chromosome. Of the Emr transconjugants, 13% were auxotrophs. Bacteroides spp. which had R751 cointegrated with Tn4351 in the chromosome did not transfer R751 or Tn4351 to E. coli HB101 or to isogenic B. uniformis, nor did the intergrated R751 mobilize pE5-2, an E. coli-Bacteroides shuttle vector that contains a transfer origin that is recognized by R751. Images PMID:3005243

  5. Budgeting in Texas Counties, 1931-1940.

    E-print Network

    Bradshaw, H. C.

    1941-01-01

    the ~arions associations of county officers, particularly the county judges and commissioners, the county auditors, and the county dmlcs. CONTENTS Introduction 5 Purpose of Study 6 Procedure 6 Legal Provisions 9 The Budget Form 11 Number of Budgets...

  6. (Dengue Fever reported in Osceola County) OSCEOLA COUNTY HEALTH DEPARTMENT

    E-print Network

    Watson, Craig A.

    (Dengue Fever reported in Osceola County) OSCEOLA COUNTY HEALTH DEPARTMENT ISSUES PUBLIC HEALTH of two cases of dengue fever in Osceola County. Dengue is one of several mosquito-borne illnesses was not acquired locally. Symptoms of dengue fever vary according to the age of the patient. Infants and young

  7. Snohomish County Biodiesel Project

    SciTech Connect

    Terrill Chang; Deanna Carveth

    2010-02-01

    Snohomish County in western Washington State began converting its vehicle fleet to use a blend of biodiesel and petroleum diesel in 2005. As prices for biodiesel rose due to increased demand for this cleaner-burning fuel, Snohomish County looked to its farmers to ���¢��������grow���¢������� this fuel locally. Suitable seed crops that can be crushed to extract oil for use as biodiesel feedstock include canola, mustard, and camelina. The residue, or mash, has high value as an animal feed. County farmers began with 52 acres of canola and mustard crops in 2006, increasing to 250 acres and 356 tons in 2008. In 2009, this number decreased to about 150 acres and 300 tons due to increased price for mustard seed.

  8. County Waterford Image Archive

    NSDL National Science Digital Library

    Using volunteer help and a locally written image management system, the Waterford County Museum in Ireland has created this collection of close to 3,000 historical images that document Waterford County and its parishes from the late 1890s to the present. The Web interface to the collection includes a number of helpful features - for those familiar with Waterford county and its place and family names, there is a search box, but for those less familiar there are several ways to browse the collection, by selecting photographer, location, image format, or subjects from drop down lists, as well as selecting parishes from a map to see the pictures associated with each. A photo of the week is posted on the site, and visitors can sign up to receive an RSS feed or, alternatively, an email message when new images are added. There are also easy ways to submit photographs for inclusion in the archive, and for locals to contribute identifying information.

  9. FATE OF TN5 MUTANTS OF ROOT GROWTH-INHIBITING PSEUDOMONAS SP. IN INTACT SOIL-CORE MICROCOSMS

    EPA Science Inventory

    Transposon Tn5 mutants of a wheat root growth-inhibiting nonfluorescent Pseudomonas sp. were inoculated into intact soil-core microcosms to determine the utility of intact soil cores for evaluating the fate and transport of microorganisms in agricultural ecosystems. ransposon Tn5...

  10. Britney Jones 3900 Wolf Lane, Germantown, TN 38138 9015170022 bjones@memphis.edu

    E-print Network

    Dasgupta, Dipankar

    paper for financial analysis Supported my internship site by providing program development assistance Honors Program Expected Graduation Date: December 2012 GPA: 3.99 Candidate for Master of Science Program Office of Academic Internships Memphis, TN Summer 2011 Improved the accounting internship

  11. ON THE DrFF,US~VE CAVITATION OF ISOLATED GRAIN BOUNDARY FACETS TN

    E-print Network

    ON THE DrFF,US~VE CAVITATION OF ISOLATED GRAIN BOUNDARY FACETS TN CREEPING POLYCRYSTALS J. R. RICE-Following concepts introduced by B. F. Dyson (Metal Sci.349 19761,the dilTusive cavitation of grain facets is considered in circumstances for which the cavitated fasts are well separated from one another. In this case

  12. Lactobacillus plantarum TN8 exhibits protective effects on lipid, hepatic and renal profiles in obese rat.

    PubMed

    Ben Salah, Riadh; Trabelsi, Imen; Hamden, Khaled; Chouayekh, Hichem; Bejar, Samir

    2013-10-01

    This study aimed to first investigate the immuno-modulatory effects of six newly isolated lactic acid bacteria (LAB) on the peripheral blood mononuclear cells (PBMC) of Wistar rats. Except for Lactobacillus plantarum TN8, all the other strains were noted to induce high levels of pro-inflammatory cytokine IL-12 and low levels of anti-inflammatory cytokine IL-10. The strains also generated low ratios of IL-10/IL-12 cytokine. Strain TN8 was, on the other hand, noted to induce an increase in anti-inflammatory IL-10 cytokine secretion rates and a decrease in pro-inflammatory IL-12, IFN-? and TNF-? cytokine production. The oral administration of TN8 improved the hepatic and urinary functions of obese rats by inducing decreases (P < 0.05) in alanine amino transferase (ALAT), gamma glutamyl transferase (GGT), plasmatic triglycerides, total cholesterol concentrations, creatinine, urea, and body weight when compared to the control group of animals that underwent an increase in aspartate amino transferase (ASAT) and high density lipoprotein (HDL). Overall, the findings indicate that strain TN8 exhibited a number of attractive properties that might open new promising opportunities for the improvement of various parameters related to animal health performance and the avoidance of antibiotics and drugs as promoting factors. PMID:23891961

  13. H-1B E-3 TN J-1 Permanent Residency (Green Card)

    E-print Network

    Wisconsin at Madison, University of

    H-1B E-3 TN J-1 Permanent Residency (Green Card) Duration 6 years with exceptions to go beyond 6 or send I-129 Mexicans--go to Consulate or send I- 129 File request with IFSS Renew green card itself with new notation on I-94 card to include the names of all employers for whom you work. Only with prior

  14. H-1B E-3 TN J-1 B-1/B-2. VWP (Visa Waiver

    E-print Network

    H-1B E-3 TN J-1 B-1/B-2. VWP (Visa Waiver Program) Permanent Residency (Green Card) Duration 6 File request with IFSS B visitors may file I-539. VWP may not extend. Renew green card every 10 years with new notation on I-94 card to include the names of all employers for whom you work. Only with prior

  15. D E C E M B E R 1 9 9 0 Technical Note TN18

    E-print Network

    Krintz, Chandra

    ­ tions. #12; Predicting Program Behavior Using Real or Estimated Profiles David W. Wall December, 1990 dD E C E M B E R 1 9 9 0 WRL Technical Note TN­18 Predicting Program Behavior Using Real or Estimated Profiles David W. Wall d i g i t a l Western Research Laboratory 100 Hamilton Avenue Palo Alto

  16. Large-Scale Mutagenesis of the Yeast Genome Using a Tn7-Derived Multipurpose Transposon

    E-print Network

    Gerstein, Mark

    Large-Scale Mutagenesis of the Yeast Genome Using a Tn7-Derived Multipurpose Transposon Anuj Kumar-Macdonald et al. 1997). Transposon mutagenesis is scalable and, therefore, equally effective as applied insertional library of yeast genomic DNA generated by in vitro mutagenesis with a multipurpose element derived

  17. Submarine Ring of Fire 2004 -Mariana Arc R/V Thomas G. Thompson, Cruise TN167

    E-print Network

    #12;Submarine Ring of Fire 2004 - Mariana Arc R/V Thomas G. Thompson, Cruise TN167 March 27 - April..............................................................................................................................................3 1.0 SUBMARINE RING OF FIRE 2004 CRUISE SUMMARY (Bob Embley..........................................................................................................25 3.0 SUBMARINE RING OF FIRE (SROF) 2004 DISCIPLINE SUMMARIES

  18. ERDC TN-EQT-10-1 Polarized Illuminator for Very-Near

    E-print Network

    US Army Corps of Engineers

    ERDC TN-EQT-10-1 May 2010 Polarized Illuminator for Very-Near Infrared Imaging by John Furey and Cliff Morgan PURPOSE: This note describes the development of a polarized illuminator system of particulate contaminants in the field (Furey et al. 2008), the need for a broad beam polarized illumination

  19. A Tn7-based broad-range bacterial cloning and expression system

    E-print Network

    Cai, Long

    . Transposon delivery and transient expression of transposase are achieved with the help of suicide plasmidsA Tn7-based broad-range bacterial cloning and expression system Kyoung-Hee Choi, Jared B Gaynor For many bacteria, cloning and expression systems are either scarce or nonexistent. We constructed several

  20. A gene and its product required for transposition of resistance transposon Tn2603.

    PubMed Central

    Tanaka, M; Harafuji, H; Yamamoto, T

    1982-01-01

    Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined through construction and characterization of various deletion mutants affecting the efficiency of transposition. Deletions were introduced in plasmid pMK1::Tn2603 by partial digestion with restriction endonuclease EcoRI in vitro. Analysis of deletion mutants showed that the inverted repeat segments at both ends of the trans-acting diffusible product(s) encoded in the right-hand side of the central portion were required for the transposition of Tn2603. An essential gene product was revealed as a protein having a molecular weight of 110,000 by analysis of polypeptides synthesized in Escherichia coli minicells. This protein was assumed to be the so-called transposase. Images PMID:6284710

  1. Transposon mutagenesis by Tn4560 and applications with avermectin-producing Streptomyces avermitilis.

    PubMed

    Ikeda, H; Takada, Y; Pang, C H; Tanaka, H; Omura, S

    1993-04-01

    The Tn3-like Streptomyces transposon Tn4560 was used to mutagenize Streptomyces avermitilis, the producer of anthelmintic avermectins and the cell growth inhibitor oligomycin. Tn4560 transposed in this strain from a temperature-sensitive plasmid to the chromosome and from the chromosome to a plasmid with an apparent frequency of about 10(-4) to 10(-3) at both 30 and 39 degrees C. Auxotrophic and antibiotic nonproducing mutations were, however, obtained only with cultures that were kept at 37 or 39 degrees C. About 0.1% of the transposon inserts obtained at 39 degrees C caused auxotrophy or abolished antibiotic production. The sites of insertion into the S. avermitilis chromosome were mapped. Chromosomal DNA fragments containing Tn4560 insertions in antibiotic production genes were cloned onto a Streptomyces plasmid with temperature-sensitive replication and used to transport transposon mutations to other strains, using homologous recombination. This technique was used to construct an avermectin production strain that no longer makes the toxic oligomycin. PMID:8384619

  2. Transposon mutagenesis by Tn4560 and applications with avermectin-producing Streptomyces avermitilis.

    PubMed Central

    Ikeda, H; Takada, Y; Pang, C H; Tanaka, H; Omura, S

    1993-01-01

    The Tn3-like Streptomyces transposon Tn4560 was used to mutagenize Streptomyces avermitilis, the producer of anthelmintic avermectins and the cell growth inhibitor oligomycin. Tn4560 transposed in this strain from a temperature-sensitive plasmid to the chromosome and from the chromosome to a plasmid with an apparent frequency of about 10(-4) to 10(-3) at both 30 and 39 degrees C. Auxotrophic and antibiotic nonproducing mutations were, however, obtained only with cultures that were kept at 37 or 39 degrees C. About 0.1% of the transposon inserts obtained at 39 degrees C caused auxotrophy or abolished antibiotic production. The sites of insertion into the S. avermitilis chromosome were mapped. Chromosomal DNA fragments containing Tn4560 insertions in antibiotic production genes were cloned onto a Streptomyces plasmid with temperature-sensitive replication and used to transport transposon mutations to other strains, using homologous recombination. This technique was used to construct an avermectin production strain that no longer makes the toxic oligomycin. PMID:8384619

  3. Transposon mutagenesis of the anaerobic commensal, Bacteroides fragilis, using the EZ?TN5 transposome

    PubMed Central

    Veeranagouda, Yaligara; Husain, Fasahath; Wexler, Hannah M.

    2012-01-01

    Genetic analysis of Bacteroides fragilis (BF) is hindered due to the lack of efficient transposon mutagenesis methods. Here we describe a simple method for transposon mutagenesis using EZ?TN5, a commercially available system that we optimized for use in BF638R. The modified EZ?TN5 transposon contains an E. coli conditional origin of replication, a kanamycin resistance gene for E. coli, an erythromycin resistance gene for BF and 19 basepair transposase recognition sequences on either ends. Electroporation of the transposome (transposon-transposase complex) into BF638R yielded 3.2± 0.35×103 CFU/?g of transposon DNA. Modification of the transposon by the BF638R restriction/modification system increased transposition efficiency 6-fold. Electroporation of the EZ?TN5 transposome results in a single copy insertion of the transposon evenly distributed across the genome of BF638R and can be used to construct a BF638R transposon library. The transposon was also effective in mutating a BF clinical isolate and a strain of the related species, B. thetaiotaomicron. The EZ?TN5 based mutagenesis described here is more efficient than other transposon mutagenesis approaches previously reported for BF. PMID:22639975

  4. INTRODUCTION Tenascin-C (TN-C), a large glycoprotein of the extracellular

    E-print Network

    Erickson, Harold P.

    it can bind chondroitin sulfate pro- teoglycans derived from smooth muscle culture (Chiquet and Fambrough chondroitin sulfate proteoglycan from brain, were shown to bind TN-C in vitro and co-localize with it in vivo cell mutants with a total block in heparan and chondroitin sulfate production failed to assemble

  5. ERDC/EL TN-10-4 Release of the Biological Control Agent

    E-print Network

    US Army Corps of Engineers

    starthistle, Centaurea solstitialis L., at Fort Hunter Liggett, an Army training installation in CaliforniaERDC/EL TN-10-4 July 2010 Release of the Biological Control Agent Puccinia jaceae var. solstitialis fungus, Puccinia jaceae var. solstitialis, as a biological control agent for management of yellow

  6. P-156 / H. Y. Mak P-156: Transflective Double Cell TN Liquid Crystal Display

    E-print Network

    for transflective liquid crystal displays, using MOUSE-LCD software. Two configurations of such DTN-LCD were simulated using a double and a single cell gap approach. DTN-LCD exhibits a high contrast ratio, wide TFT-LCD. Keywords: transflective LCD; double cell TN-LCD, DTN. 1. INTRODUCTION Transflective LCDs

  7. Approved for public release; distribution is unlimited. ERDC/TN APCRP-BC-24

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited. ERDC/TN APCRP-BC-24 October 2011 Effect is primarily managed using herbicide applications and to some extent by using specialized harvesting equipment collectively known as mechanical control. The recent appearance of herbicide resistance in some populations

  8. Approved for public release; distribution is unlimited ERDC/TN APCRP-BC-22

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited ERDC/TN APCRP-BC-22 May 2011 Progress Report led to the development of herbicide resistance to the systemic ingredient fluridone (Michel et al of aquatic weeds and the emergence of herbicide resistance, using biological control for the management

  9. Approved for public release; distribution is unlimited. ERDC/TN APCRP-CC-15

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited. ERDC/TN APCRP-CC-15 March 2011 Endothall registered aquatic herbicides and experimental use permit (EUP) herbicides are currently being evaluated herbicides available for weed control in irrigation canals. This study was conducted because of the direct

  10. Approved for public release; distribution is unlimited. ERDC/EL TN-13-1

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited. ERDC/EL TN-13-1 April 2013 Chemical Control, Ernest Kafcas, and John Schafer PURPOSE: This field demonstration evaluated herbicide management.g., herbicide applications, disking, burning, flooding) have been undertaken to reduce the phragmites

  11. Approved for public release; distribution is unlimited. ERDC/EL TN-11-3

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited. ERDC/EL TN-11-3 August 2011 Evaluation of Commercially Available Herbicide Mixes for Control of Rosette Stage Yellow Starthistle (Centaurea solstitialis and clopyralid. Although these herbicides have been widely used and provide excellent control both pre-and post

  12. Approved for public release; distribution is unlimited. ERDC/TN APCRP-CC-16

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited. ERDC/TN APCRP-CC-16 May 2011 Utilizing a Small-scale Primary Screening Method to Evaluate Activity of the Bleaching Herbicide Topramezone evaluating potential use patterns of new aquatic herbicides, it is important to determine concentrations

  13. Approved for public release; distribution is unlimited. ERDC/TN APCRP-CC-18

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited. ERDC/TN APCRP-CC-18 March 2013 Small-scale Primary Screening Method to Predict Impacts of the Herbicide Flumioxazin on Native and Invasive Emergent aquatic herbicide, flumioxazin, against 23 native emergent plant species and five invasive emergent

  14. Approved for public release; distribution is unlimited. ERDC/TN APCRP-BC-28

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited. ERDC/TN APCRP-BC-28 June 2012 Progress of herbicide resistance. Resistance to the systemic herbicide, fluridone, has been reported in hydrilla@wbgcas.cn 2 U.S. Department of Agriculture ­ Agriculture Research Service (USDA-ARS), Australian Biological

  15. Approved for public release; distribution is unlimited. ERDC/TN APCRP-BC-33

    E-print Network

    US Army Corps of Engineers

    Approved for public release; distribution is unlimited. ERDC/TN APCRP-BC-33 July 2014 The Use Department of Agriculture/Natural Resources Conservation Service (USDA/NRCS) 2012). Waterhyacinth is capable exceeds $4 million.3 Primary control methods include the use of herbicides and release of insect

  16. US Geological Survey, Great Smokies Field Station, 1314 Cherokee Orchard Road, Gatlinburg, TN 37738. 2

    E-print Network

    Mathis, Wayne N.

    1 US Geological Survey, Great Smokies Field Station, 1314 Cherokee Orchard Road, Gatlinburg, TN, Washington, DC 20013-7012. 3 Department of Entomology, Purdue University, West Lafayette, IN 47904. 4). In addition, much of the south side of GSMNP borders Fontana Lake, a large Tennessee 159 The Great Smoky

  17. Purchasing in Texas Counties.

    E-print Network

    Hervey, E. J.; Bradshaw, H. C.

    1944-01-01

    8 r3' L \\, & #5, CnLpL"; 3' --%I k? TEXAS AGRICULTURAL EXPERIMENT STATION A. B. CONNER, Director College Station, Texas BULLETIN NO. 653 JULY 1944 PURCHASING IN TEXAS COUNTIES H. C. BRADSEAW and E. J, HERVEY Division of Farm and Ranch... Economics 00.: .*- AGRICULTURAL AND MECHANICAL COLLEGE OF TEXAS GIBB GILCHRIST, President D-21-744-4500 The use of good purchasing procedures in a number of Texas counties is reducing the cost price of materials, supplies and equipment by 15 to 20 per...

  18. 2013 County Commissioner Satisfaction Survey

    E-print Network

    commissioner is expected to complete the CSU Extension survey. Per-county responses (N = 56) are calculated surveys was 84, for an overall response rate of 39%. The per-county response rate was excellent, with 562013 County Commissioner Satisfaction Survey: CSU Extension Services in Colorado Survey Results

  19. i MARIN COUNTY~ ....-_.-"".~

    E-print Network

    : Initiate an amendment to the Building Code updating the County's green building requirements by taking by requiring green building through the building permit review Progess. The adopted Green Building Ordinance that Title 24, Part 6 of the California Building Code are the minimum energy efficiency requirements

  20. Wasp Watchers Albany County

    E-print Network

    Walter, M.Todd

    { Wasp Watchers Albany County Finding the Wasp that hunts the Emerald Ash Borer #12;Cerceris fumipennis is a solitary ground nesting wasp. The female wasp stocks her nest with Buprestid beetles ,this as 80% of the Cerceris fumipennis colonies daily catch. · This wasp is very wide spread in the United

  1. 7 CFR 7.11 - County committee members.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...county executive director, other employee of the county committee, or the county agricultural extension agent for the county. If the county agricultural extension agent is not selected as secretary to the county committee, that person...

  2. 77 FR 59573 - Proposed Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ...Rogersville, TN, and Virginia Highlands Airport...The Tri-Cities Class D airspace description...separation of existing Class E airspace surrounding Virginia Highlands Airport...Rogersville, TN, and Virginia Highlands Airport...amend existing Class E airspace...

  3. 78 FR 43827 - Irish Potatoes Grown in Modoc and Siskiyou Counties, California, and in All Counties in Oregon...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-22

    ...Siskiyou Counties, California, and in All Counties in Oregon, Except Malheur County...Siskiyou Counties, California, and in all counties in Oregon, except Malheur County...at: http://www.regulations.gov. All comments submitted in response to this...

  4. Effects of HCM cTnI mutation R145G on troponin structure and modulation by PKA phosphorylation elucidated by molecular dynamics simulations.

    PubMed

    Lindert, Steffen; Cheng, Yuanhua; Kekenes-Huskey, Peter; Regnier, Michael; McCammon, J Andrew

    2015-01-20

    Cardiac troponin (cTn) is a key molecule in the regulation of human cardiac muscle contraction. The N-terminal cardiac-specific peptide of the inhibitory subunit of troponin, cTnI (cTnI(1-39)), is a target for phosphorylation by protein kinase A (PKA) during ?-adrenergic stimulation. We recently presented evidence indicating that this peptide interacts with the inhibitory peptide (cTnl(137-147)) when S23 and S24 are phosphorylated. The inhibitory peptide is also the target of the point mutation cTnI-R145G, which is associated with hypertrophic cardiomyopathy (HCM), a disease associated with sudden death in apparently healthy young adults. It has been shown that both phosphorylation and this mutation alter the cTnC-cTnI (C-I) interaction, which plays a crucial role in modulating contractile activation. However, little is known about the molecular-level events underlying this modulation. Here, we computationally investigated the effects of the cTnI-R145G mutation on the dynamics of cTn, cTnC Ca(2+) handling, and the C-I interaction. Comparisons were made with the cTnI-R145G/S23D/S24D phosphomimic mutation, which has been used both experimentally and computationally to study the cTnI N-terminal specific effects of PKA phosphorylation. Additional comparisons between the phosphomimic mutations and the real phosphorylations were made. For this purpose, we ran triplicate 150 ns molecular dynamics simulations of cTnI-R145G Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), cTnI-R145G/S23D/S24D Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), and cTnI-R145G/PS23/PS24 Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), respectively. We found that the cTnI-R145G mutation did not impact the overall dynamics of cTn, but stabilized crucial Ca(2+)-coordinating interactions. However, the phosphomimic mutations increased overall cTn fluctuations and destabilized Ca(2+) coordination. Interestingly, cTnI-R145G blunted the intrasubunit interactions between the cTnI N-terminal extension and the cTnI inhibitory peptide, which have been suggested to play a crucial role in modulating troponin function during ?-adrenergic stimulation. These findings offer a molecular-level explanation for how the HCM mutation cTnI-R145G reduces the modulation of cTn by phosphorylation of S23/S24 during ?-adrenergic stimulation. PMID:25606687

  5. Burleson County State of the Community Report 

    E-print Network

    Jiang, Ying; Thapa, Jeewasmi; Safarova, Bara

    2015-01-01

    County. Title page image courtesy of: http://www.burlesoncountytx.com State of the Community Report: Burleson County Ying Jiang | Jeewasmi Thapa | Bara Safarova 3 1. Introduction 1.1. Basic information Burleson County is located in east central... of the county. Figure 5.3: Critical facilities distribution in Burleson County (Source: Burleson County Hazard Mitigation, 2013) 5.5. Oil and gas wells and pipes The petroleum in Burleson County is embedded in the Austin...

  6. [Effect of alpha-(p-thenoylphenyl)-propionic acid (TN-762) on acute inflammatory reactions and prostaglandin biosynthesis].

    PubMed

    Fujimura, H; Tsurumi, K; Nakayama, T; Kokuba, S; Hiramatsu, Y; Tamura, Y

    1981-03-01

    A series of compounds with the general structure of phenylpropionic acid was originally synthesized for anti-inflammatory screening. The title compound, TN-762, showed marked anti-inflammatory and analgesic activities and was less toxic. This compound was the same as suprofen which was reported by Janssen to have potent anti-inflammatory and anti-writhing activities. Effects of TN-762 on acute inflammatory reactions and prostaglandin biosynthesis were investigated in animal models and findings compared to those of ketoprofen and indomethacin. TN-762 showed a dose-dependent inhibition at low doses of 5-20 mg/kg, p.o. on an increased vascular permeability induced by histamine in rats and by acetic acid in mice and carrageenin-induced paw edema in rats. The anti-inflammatory activity of TN-762 was much the same as that of ketoprofen and indomethacin. The inhibitory effect of TN-762 on carrageenin-induced paw edema was not affected by successive administration for 14 days and/or by adrenalectomy. The compound was more active than the two reference compounds in inhibiting ultraviolet erythema in guinea pigs. TN-762 inhibited markedly the arachidonic acid potentiation of carrageenin-induced edema in rat paw, the sudden death following intraveneous administration of arachidonic acid to rabbits and the diarrhea produced by endotoxin in mice, all considered to be induced by biosynthetic prostaglandins. The activities of TN-762 were the same or were more potent than those of ketoprofen and indomethacin. On the contrary, the ulcerogenic activity of TN-762 on the gastrointestinal tract in rats was significantly less than that of ketoprofen and indomethacin. From the above results, TN-762 proved to be a potent inhibitor of acutely-induced inflammation and of prostaglandin biosynthesis, however, the ulcerogenic effects were comparatively diminished. PMID:7052360

  7. 75 FR 49016 - County of Greenville, S.C.-Acquisition Exemption-Greenville County Economic Development Corporation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ...Exemption--Greenville County Economic Development Corporation The County of...acquire from Greenville County Economic Development Corporation (GCEDC) approximately...See Greenville County Economic Development...

  8. Lake County Paratransit Survey Final Report Prepared for the Lake County Coordinated Transportation Services Committee

    E-print Network

    Illinois at Chicago, University of

    Lake County Paratransit Survey Final Report Prepared for the Lake County Coordinated Transportation............................................................................25 4. Appendix A: Lake County Paratransit Survey Instrument.................................27 #12;2 Lake County Paratransit Survey Final Report Executive Summary The Urban Transportation Center

  9. County Business Patterns 1995

    NSDL National Science Digital Library

    The US Census Bureau has made available the state publications for the 1995 version of its annual County Business Patterns 1995 series (Adobe Acrobat [.pdf] format only). Data available include employment, payroll, and number of establishments by Standard Industrial Code category. Establishments are further broken down by nine employment size classes ranging from 1-4 to 1,000 or more. The US volume is forthcoming. Complete 1993 and 1994 (also .pdf format) series are available at the site.

  10. ROLE OF NA+ IN TRANSPORT OF HG2+ AND INDUCTION OF THE TN21 "MER" OPERON

    EPA Science Inventory

    The effects of sodium ions on the uptake of Hg 2 + and induction of the TN21 mer operon were studied using E. coli HMS174 harboring the reporter plasmids pRB28 and pOS14. lasmid pRB28 carries merRT' and pOS14 carries merRTPC of the mer operon, both cloned upstream of a promoterle...

  11. Design analysis report for the TN-WHC cask and transportation system

    SciTech Connect

    Brisbin, S.A., Fluor Daniel Hanford

    1997-02-13

    This document presents the evaluation of the Spent Nuclear Fuel Cask and Transportation System. The system design was developed by Transnuclear, Inc. and its team members NAC International, Nelson Manufacturing, Precision Components Corporation, and Numatec, Inc. The cask is designated the TN-WHC cask. This report describes the design features and presents preliminary analyses performed to size critical dimensions of the system while meeting the requirements of the performance specification.

  12. Tn5 insertion mutants of Pseudomonas aeruginosa deficient in surface expression of ferripyochelin-binding protein

    SciTech Connect

    Sokol, P.A.

    1987-07-01

    Transposon (Tn5) insertion mutants were isolated in Pseudomonas aeruginosa PAO. These mutants were screened for expression of the ferripyochelin-binding protein with monoclonal antibody in a whole-cell immunoblot assay. Fourteen mutants were identified which did not express ferripyochelin-binding protein on the cell surface. These mutants did not take up /sup 59/Fe-labeled pyochelin and grew slowly in the presence of iron chelators.

  13. Tn5-induced lipopolysaccharide mutations in Bordetella pertussis that affect outer membrane function

    Microsoft Academic Search

    M. L. Turcotte; Denis Martin; Bernard R. Brodeur; Mark S. Peppler

    1997-01-01

    An LPSB-specific mAb was used to screen for ten TnS insertion mutants of Bordetella pertussis which have LPS which is phenotypically distinct from either wild-type LPSAB or LPSB. Silver-stained SDS-PAGE gels showed nine different LPS phenotypes, six of which contain two clinically undocumented LPS bands, designated lntA and lntB based on their proximity to the LPSA and LPSB bands, respectively.

  14. Transposon Tn 21 encodes a RecA -independent site-specific integration system

    Microsoft Academic Search

    Eduardo Martinez; Fernando de la Cruz

    1988-01-01

    The IncW plasmid R388 and the DNA region of Tn21 containing the Smr and the Sur genes are capable of RecA-independent recombination. This recombination occurs at a relatively high frequency (up to 10-4 recombinants per recipient molecule) and results in integration of the two plasmids. No detectable repeats are formed in the process. The crossover points have been confined to

  15. ESCHERICHIA COLI K-12 Auxotrophs Induced by Insertion of the Transposable Element Tn5

    PubMed Central

    Shaw, Karen J.; Berg, Claire M.

    1979-01-01

    The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess. PMID:395018

  16. The Sugar Creek zinc deposit, Jackson Co. TN -- Exploration history, geology and mineralization

    Microsoft Academic Search

    G. Reinbold; A. V. Moran; D. L. Stevens

    1993-01-01

    During the 60's and 70's zinc exploration of central TN and KY was active. The Sugar Creek Project was one of several investigated by Exxon. The discovery hole, Cu 15, was drilled in early 1973. The Sugar Creek Zinc Deposit was acquired by Independence Mining Co. in 1986 and I.M.C. has subsequently completed additional drilling, both stepout and confirmation holes.

  17. Morphological and Functional Diversity of Ectomycorrhizal Fungi on Roan Mountain (NC\\/TN)

    Microsoft Academic Search

    Claire Bird; Coleman McCleneghan

    2005-01-01

    A comparison of ectomycorrhizal morphotypes and hypogeous fungi (truffles and false-truffles) in northern hardwood and spruce-fir forests on Roan Mountain (NC\\/TN) was performed to increase our knowledge of the fungal communities in the Southern Appalachian high elevation forests. These forests are home to an endangered subspecies and mycophagist, the Carolina northern flying squirrel (Glaucomys sabrinus coloratus), as well as the

  18. 77 FR 66480 - Final Environmental Impact Statement, Narrows Project, Sanpete County, Utah

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-05

    ...diversion of up to 5,400 acre-feet of water annually from Gooseberry Creek drainage...Sevier River drainage basin. The water right used under the action alternatives...alternatives, the SWCD's conversion of water from irrigation to municipal...

  19. Incised valley fill interpretation for Mississippian Black Hand Sandstone, Appalachian Basin, USA: Implications for glacial eustasy at Kinderhookian Osagean (Tn2 Tn3) boundary

    NASA Astrophysics Data System (ADS)

    Matchen, David L.; Kammer, Thomas W.

    2006-09-01

    Lower Mississippian strata of east-central Ohio are predominantly fine-grained marine deposits of the Cuyahoga and Logan formations. Within these sediments is the Black Hand Sandstone of the Cuyahoga Formation. The Black Hand Sandstone is a multistory, crossbedded, coarse-grained conglomeratic sandstone. The contact between the Black Hand Sandstone and the subjacent Cuyahoga Formation is sharp and scoured, with intraclasts of the Cuyahoga Formation incorporated into the basal Black Hand Sandstone. The Black Hand Sandstone was previously thought to represent a distributary channel deposit; however, the combination of lithofacies and architectural elements indicates deposition in a braided stream setting. The Cuyahoga Formation was deposited in a shallow marine setting. The erosional basal contact of the Black Hand Sandstone and the juxtaposition of fluvial and marine sediments suggests a sequence boundary. The geographic distribution of the Black Hand Sandstone combined with the evidence for a sequence boundary suggests deposition in an incised valley. The age of the Black Hand Sandstone is key to inferring the causes of valley incision. The Black Hand Sandstone is nearly devoid of body fossils, necessitating a biostratigraphic analysis of the surrounding Cuyahoga and Logan formations. Analysis indicates the Logan Formation is early Osagean age. Data from the Cuyahoga Formation suggest a Kinderhookian age with a possible transition to the Osagean in the uppermost Cuyahoga Formation. This constrains the age of the Black Hand Sandstone to the transition at the Kinderhookian-Osagean boundary. Recent reports indicate late Kinderhookian (Tournaisian, Tn2) Gondwanan glaciation based upon tillites and sharp excursions in stable-isotope curves. A glacio-eustatic fall in sea level is inferred to have caused incision of the Cuyahoga Formation, followed by deposition of the Black Hand Sandstone and Logan Formation during the subsequent sea level rise. The associated unconformity correlates to the sequence boundary at the Kinderhookian-Osagean boundary in the stratotype area of North America, and the correlative Tn2-Tn3 boundary worldwide, supporting the hypothesis of a global eustatic event at this time.

  20. Heritage Awareness in County Wicklow

    Microsoft Academic Search

    Anne Dagg

    2008-01-01

    This research project investigated the community’s current level of heritage awareness in County Wicklow. The study was initiated by Wicklow County Council and the Heritage Council in response to objective 1, action 1.2 of the County Wicklow heritage plan 2004-2008, which pointed to the need to undertake a study to determine public attitudes towards heritage and to gauge the current

  1. 78 FR 25403 - Proposed Amendment of Class E Airspace; Dayton, TN, Establishment of Class E Airspace; Cleveland...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ...SIAPs at the airport. Information regarding Bradley Memorial Hospital would be added to the Cleveland...operations. Bradley Memorial Hospital information would be added to the Cleveland...listing as Bradley Memorial Hospital, Cleveland, TN, to...

  2. 76 FR 34799 - Permanent Dam Safety Modification at Cherokee, Fort Loudoun, Tellico, and Watts Bar Dams, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-14

    ...TENNESSEE VALLEY AUTHORITY Permanent Dam Safety Modification at Cherokee, Fort Loudoun, Tellico, and Watts Bar Dams, TN AGENCY: Tennessee Valley Authority...permanent modifications to the existing dam facilities at Cherokee, Fort...

  3. Retrofit Savings for Brazos County 

    E-print Network

    Baltazar-Cervantes, J. C.; Shao, X.; Claridge, D. E.

    2001-01-01

    &M University System BRAZOS COUNTY SumarySav2000m.xls 10/23/2001 BRAZOS COUNTY SumarySav2000(kW)m.xls 10/23/2001 Summary of energy savings from May 2000 to April 2001 Individual Reports BRAZOS COUNTY ENERGY SYSTEMS LABORATORY Texas Engineering Experiment Station... ENERGY SYSTEMS LABORATORY Texas Engineering Experiment Station Texas A&M University System SummaryModeling.xls 10/20/01 Summary of the electricity use and demand final modeling for Brazos County ...

  4. Digital Atlas of Texas Counties

    NSDL National Science Digital Library

    Created by the Center for Geosptial Technology at Texas Tech University, this digital atlas is a fantastic find. It brings together information about all of Texas's counties, including satellite relief maps and data sets about the roads, rivers, lakes, and other features in each area. Visitors can use the interactive map to click on a county of interest, or use the drop down menu to select a region. Once users select a county, they can download information about it for future use. The site also contains a number of Featured Links to resources from the U.S. Census Bureau, the Texas Association of Counties, and several tourism agencies.

  5. Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn552'phoA In Vitro Transposition System

    Microsoft Academic Search

    MIRIAM BRAUNSTEIN; THOMAS J. GRIFFIN; JORDAN I. KRIAKOV; SARAH T. FRIEDMAN; NIGEL D. F. GRINDLEY; WILLIAM R. JACOBS

    2000-01-01

    Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogen- esis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552*phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the transposon inserts randomly into target DNA. M. tuberculosis genomic libraries were targeted with Tn552*phoA trans-

  6. Protective action of tetramethylpyrazine phosphate against dilated cardiomyopathy in cTnTR141W transgenic mice

    Microsoft Academic Search

    Hai-ping Zhao; Dan Lü; Wei Zhang; Li Zhang; Shu-mei Wang; Chun-mei Ma; Chuan Qin; Lian-feng Zhang

    2010-01-01

    Aim:Dilated cardiomyopathy (DCM) is the most common cause of heart failure, and pharmacological intervention is not currently available. Here we investigate the effect of tetramethylpyrazine phosphate (TMPP) on the progression of DCM in the cTnTR141W transgenic mouse model.Methods:The cTnTR141W transgenic mice aged 2 months were divided into model group and TMPP group, whereas age-matched nontransgenic mice were used as wild-type

  7. Genetic map of the bacteriocinogenic plasmid CLO DF13 derived by insertion of the transposon Tn 901

    Microsoft Academic Search

    P. M. Andreoli; N. Overbeeke; E. Veltkamp; J. D. A. Embden; H. J. J. Nijkamp

    1978-01-01

    An ampicillin transposon Tn901 was used as a “mutagen” to isolate insertion mutants of the bacteriocinogenic plasmid Clo DF13. By combining the obtained heteroduplex and restriction maps of the Clo DF13::Tn901 plasmids (van Embden et al., 1977b) with their polypeptide pattern in minicells, we were able to map five genes on the Clo DF13 genome. These five genes designated A

  8. The Listeria monocytogenes transposon Tn6188 provides increased tolerance to various quaternary ammonium compounds and ethidium bromide.

    PubMed

    Müller, Anneliese; Rychli, Kathrin; Zaiser, Andreas; Wieser, Cornelia; Wagner, Martin; Schmitz-Esser, Stephan

    2014-12-01

    Tolerance of the foodborne pathogen Listeria monocytogenes to sublethal concentrations of disinfectants has been frequently reported. Particularly, quaternary ammonium compounds (QACs) such as benzalkonium chloride (BC) are often used in disinfectants and also as antiseptics in food industry and hospitals. Recently, we described Tn6188, a novel transposon in L. monocytogenes harbouring the transporter QacH, a molecular mechanism leading to increased tolerance to BC. In this study, we investigated the presence of Tn6188 within the genus Listeria spp. Our screening indicates that the distribution of Tn6188 may be limited to L. monocytogenes. We confirm that QacH is responsible for the observed increase in tolerance by complementation of a qacH deletion mutant and introducing qacH in a Tn6188 negative strain. We investigated the transporter's substrate spectrum by determining minimal inhibitory concentrations (MICs) and showed that QacH also confers higher tolerance towards other QACs and ethidium bromide (EtBr). This result was supported by increased expression of qacH in the presence of the various substrates as determined by quantitative reverse transcriptase PCR (qRT-PCR). In addition, we detected expression of a Tn6188 transposase gene and circular forms of Tn6188, suggesting activity and possible transfer of this transposon. PMID:25312720

  9. Taiwan Nantou County earthquake 0327 Taiwan Nantou County earthquake

    E-print Network

    Taiwan Nantou County earthquake 20130327 1 #12;0327 Taiwan Nantou County earthquake Source, Intensity 5 #12;I II III IV V VI VII Intensity Shake map of the March 27 Earthquake The peak ground and Technology Center for Disaster Reduction (NCDR) #12;Earthquake Response and Evacuation are a Part of Students

  10. Rosebud County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 4.6% 4.1% 6.0% All Sites Cancer 472.3 (Region 1) 455.5 543.2 1 Community County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Rosebud County Secondary Data Analysis

  11. Orange County Medical Association Headquarters

    E-print Network

    Cramer, Karina

    Anaphylaxis" Michael Ritter, MD Mission Hospital and Medical Center Assistant Clinical Professor UniversityOrange County Medical Association Headquarters 17322 Murphy Avenue Irvine, CA 92614 happelbaum@ocma.org 949) 398-8100 In partnership with the Orange County Medical Association and the UC Irvine School

  12. Treasure County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    of Death County1 Montana1,2 Nation2 1. Cancer 2. Cerebrovascular Disease 3. Heart Disease, Pneumonia, CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Treasure County Dept of Health and Human Services (2010) 2 Center for Disease Control and Prevention (CDC), National

  13. Systematic sequencing of cDNA clones using the transposon Tn5

    PubMed Central

    Shevchenko, Yuriy; Bouffard, Gerard G.; Butterfield, Yaron S. N.; Blakesley, Robert W.; Hartley, James L.; Young, Alice C.; Marra, Marco A.; Jones, Steven J. M.; Touchman, Jeffrey W.; Green, Eric D.

    2002-01-01

    In parallel with the production of genomic sequence data, attention is being focused on the generation of comprehensive cDNA-sequence resources. Such efforts are increasingly emphasizing the production of high-accuracy sequence corresponding to the entire insert of cDNA clones, especially those presumed to reflect the full-length mRNA. The complete sequencing of cDNA clones on a large scale presents unique challenges because of the generally small, yet heterogeneous, sizes of the cloned inserts. We have developed a strategy for high-throughput sequencing of cDNA clones using the transposon Tn5. This approach has been tailored for implementation within an existing large-scale ‘shotgun-style’ sequencing program, although it could be readily adapted for use in virtually any sequencing environment. In addition, we have developed a modified version of our strategy that can be applied to cDNA clones with large cloning vectors, thereby overcoming a potential limitation of transposon-based approaches. Here we describe the details of our cDNA-sequencing pipeline, including a summary of the experience in sequencing more than 4200 cDNA clones to produce more than 8 million base pairs of high-accuracy cDNA sequence. These data provide both convincing evidence that the insertion of Tn5 into cDNA clones is sufficiently random for its effective use in large-scale cDNA sequencing as well as interesting insight about the sequence context preferred for insertion by Tn5. PMID:12034835

  14. Mutagenesis of dimeric plasmids by the transposon. gamma. delta. (Tn1000)

    SciTech Connect

    Liu, L.; Berg, C.M. (Univ. of Connecticut, Storrs (USA))

    1990-05-01

    The Escherichia coli F factor mediates conjugal transfer of a plasmid such as pBR322 primarily by replicative transposition of transposon {gamma}{delta} (Tn1000) from F to that plasmid to form a cointegrate intermediate. Although resolution of this cointegrate always yields a plasmid containing a single {gamma}{delta} insertion, the occasional recovery of transposon-free plasmids after connuugal transfer has led to alternative hypotheses for F mobilization. The authors show here that {gamma}{delta}-free plasmids are found after F-mediated conjugal transfer only when the donor plasmid is a dimer and the recipient is Rec{sup +}.

  15. Direct repeats flanking the Bacteroides transposon Tn4351 are insertion sequence elements.

    PubMed Central

    Hwa, V; Shoemaker, N B; Salyers, A A

    1988-01-01

    The clindamycin-erythromycin resistance (Ccr Emr) region of the Bacteroides transposon Tn4351 is flanked by direct repeats. This study showed that the direct repeats are insertion sequence (IS) elements. Although both IS elements can mediate transfer of the chloramphenicol (Cmr) marker on pBR328 by cointegrate formation with the conjugal IncW plasmid R388, IS4351R-mediated transfer of Cmr occurred at a consistently lower frequency than did the transfer mediated by IS4351L. Analysis of plasmids from the resultant transconjugants revealed IS-mediated activities such as deletions, tandem duplication of IS4351L, and excision of IS4351R. Images PMID:2826402

  16. Metabolite profiling reveals abiotic stress tolerance in Tn5 mutant of Pseudomonas putida.

    PubMed

    Chaudhry, Vasvi; Bhatia, Anil; Bharti, Santosh Kumar; Mishra, Shashank Kumar; Chauhan, Puneet Singh; Mishra, Aradhana; Sidhu, Om Prakash; Nautiyal, Chandra Shekhar

    2015-01-01

    Pseudomonas is an efficient plant growth-promoting rhizobacteria (PGPR); however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5) mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC) and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE) and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS) pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA) on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic stress tolerant metabolites from the NBRI1108T suggest that Tn5 mutagenesis enhanced tolerance towards high temperature and drought. Tolerance to drought was further confirmed in greenhouse experiments with maize as host plant, where NBRI1108T showed relatively high biomass under drought conditions. PMID:25629312

  17. King County Snapshots

    NSDL National Science Digital Library

    This fine photographic archive serves as both a great repository of visual historical documentation of the King County area (which includes Seattle) in the state of Washington, as a good example of a collaborative partnership between various organizations. The partnership includes bringing together the visual collections of ten small historical organizations in tandem with the University of Washington and Seattle's Museum of History and Industry. Visitors can read about the working relationship between the organizations, view training materials from three workshops (such as one on image selection), and examine a list of online resources on subjects such as scanning digital images and metadata guidelines. Lest one forget the extensive visual materials (over 12,000 items as of the last count), visitors may search across each distinct collection, or elect to browse through each one individually. To get visitors started with using the archive, a number of sample searches are provided here as well.

  18. Tennessee Higher Education County Profiles, 2008

    ERIC Educational Resources Information Center

    Tennessee Higher Education Commission, 2008

    2008-01-01

    This report presents a localized perspective on Tennessee higher education, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; (3) number of county residents enrolled in Tennessee public institutions; and (4) number of county residents participating in the…

  19. Identification and cloning of genes from Porphyromonas gingivalis after mutagenesis with a modified Tn4400 transposon from Bacteroides fragilis.

    PubMed

    Chen, T; Dong, H; Tang, Y P; Dallas, M M; Malamy, M H; Duncan, M J

    2000-01-01

    Porphyromonas gingivalis is a gram-negative, black-pigmented, oral anaerobe strongly associated with adult periodontitis. Previous transposon mutagenesis studies with this organism were based on the Bacteroides transposon Tn4351. Characterization of Tn4351-disrupted genes by cloning has not been an efficient way to analyze large numbers of mutants and is further complicated by the high rate of cointegration of the suicide delivery vector containing Tn4351. In this study, we mutagenized P. gingivalis with a modified version of the Bacteroides fragilis transposon Tn4400. Plasmid pYT646B carrying the transposon was mobilized from Escherichia coli to P. gingivalis ATCC 33277 by conjugation. Both normal and inverse transposition frequencies were similar (3 x 10(-8)). However, the inverse transposon (Tn4400') contains a pBR322 replicon and a beta-lactamase gene; thus, the cloning of disrupted genomic DNAs from inverse transposition mutants was easily accomplished after ligation of genomic fragments and transformation into E. coli. Thousands of transconjugants could be obtained in a single mating experiment, and inverse transposition was random as demonstrated by Southern hybridization. By this procedure the disrupted genes from P. gingivalis pleiotropic mutants were quickly cloned, sequenced, and identified. PMID:10603421

  20. Acquisition of multidrug resistance transposon Tn6061 and IS6100-mediated large chromosomal inversions in Pseudomonas aeruginosa clinical isolates.

    PubMed

    Coyne, Sébastien; Courvalin, Patrice; Galimand, Marc

    2010-05-01

    Pseudomonas aeruginosa is a major human opportunistic pathogen, especially for patients in intensive care units or with cystic fibrosis. Multidrug resistance is a common feature of this species. In a previous study we detected the ant(4')-IIb gene in six multiresistant clinical isolates of P. aeruginosa, and determination of the environment of the gene led to characterization of Tn6061. This 26 586 bp element, a member of the Tn3 family of transposons, carried 10 genes conferring resistance to six drug classes. The ant(4')-IIb sequence was flanked by directly repeated copies of ISCR6 in all but one of the strains studied, consistent with ISCR6-mediated gene acquisition. Tn6061 was chromosomally located in six strains and plasmid-borne in the remaining isolate, suggesting horizontal acquisition. Duplication-insertion of IS6100, that ended Tn6061, was responsible for large chromosomal inversions. Acquisition of Tn6061 and chromosomal inversions are further examples of intricate mechanisms that contribute to the genome plasticity of P. aeruginosa. PMID:20110294

  1. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

    PubMed Central

    Sioud, Samiha; Aigle, Bertrand; Karray-Rebai, Ines; Smaoui, Slim; Bejar, Samir; Mellouli, Lotfi

    2009-01-01

    Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch. PMID:19547659

  2. In-house preparation of lectin panel and detection of Tn polyagglutination

    PubMed Central

    Das, Sudipta Sekhar

    2015-01-01

    Polyagglutination is a condition in which red cells are agglutinated by ABO-compatible adult human sera, but not by cord blood sera and may be acquired or inherited. Lectins are invaluable reagents in the investigation of red cells polyagglutination. We prepared in-house lectin panel and confirmed Tn polyagglutination in a pregnant lady. The lady was anemic and refused blood transfusion elsewhere due to serological discrepancy. We found ABO discrepancy and an incompatible minor cross-match in the initial investigation and suspected polyagglutination. Confirmation of polyagglutination was done using adult and cord sera. We then used the in-house lectin panels to detect the type of polyagglutination. The agglutination pattern with the various lectins was suggestive of Tn polyagglutination, which was further supported by the enzyme study. Most blood banks in India lack commercial lectin panels because of cost and procurement difficulty. Lectins play an important role in the diagnosis and differentiation of polyagglutination and immunohematological management of patient. The important and basic lectins can be prepared in-house using specific raw seeds following standardized protocol. PMID:25722587

  3. Isolation and characterization of transposon Tn5-induced symbiotic mutants of Rhizobium loti.

    PubMed Central

    Chua, K Y; Pankhurst, C E; Macdonald, P E; Hopcroft, D H; Jarvis, B D; Scott, D B

    1985-01-01

    Rhizobium loti NZP2037 and NZP2213, each cured of its single large indigenous plasmid, formed effective nodules on Lotus spp., suggesting that the symbiotic genes are carried on the chromosome of these strains. By using pSUP1011 as a vector for introducing transposon Tn5 into R. loti NZP2037, symbiotic mutants blocked in hair curling (Hac), nodule initiation (Noi), bacterial release (Bar), and nitrogen fixation (Nif/Cof) on Lotus pedunculatus were isolated. Cosmids complementing the Hac, Noi, and Bar mutants were isolated from a pLAFR1 gene library of NZP2037 DNA by in planta complementation and found to contain EcoRI fragments of identical sizes to those into which Tn5 had inserted in the mutants. The cosmids that complemented the mutants of these phenotypic classes did not share common fragments, nor did cosmids that complemented four mutants within the Noi class, suggesting that these symbiotically important regions are not tightly linked on the R. loti chromosome. Images PMID:2984178

  4. Characterization of the Tn916 conjugative transposon in a food-borne strain of Lactobacillus paracasei.

    PubMed

    Devirgiliis, Chiara; Coppola, Doriana; Barile, Simona; Colonna, Bianca; Perozzi, Giuditta

    2009-06-01

    Food-borne antibiotic-resistant lactic acid bacteria have received growing attention in the past few years. We have recently identified tetracycline-resistant Lactobacillus paracasei in samples of milk and natural whey starter cultures employed in the manufacturing process of a typical Italian fermented dairy product, Mozzarella di Bufala Campana. In the present study, we have characterized at the molecular level the genetic context of tetracycline resistance determinants in these natural strains, which we have identified as tet(M). This gene was present in 21 independent isolates, whose fingerprinting profiles were distributed into eight different repetitive extragenic palindromic groups by cluster analysis. We provide evidence that the gene is associated with the broad-host, conjugative transposon Tn916, which had never before been described to occur in L. paracasei. PCR analysis of four independent isolates by use of specifically designed primer pairs detected the presence of a circular intermediate form of the transposon, carrying a coupling sequence (GGCAAA) located between the two termini of Tn916. This novel coupling sequence conferred low conjugation frequency in mating experiments with the recipient strain JH2-2 of Enterococcus faecalis. PMID:19395574

  5. Separate structural and functional domains of Tn4430 transposase contribute to target immunity.

    PubMed

    Lambin, Michaël; Nicolas, Emilien; Oger, Cédric A; Nguyen, Nathan; Prozzi, Deborah; Hallet, Bernard

    2012-02-01

    Like other transposons of the Tn3 family, Tn4430 exhibits target immunity, a process that prevents multiple insertions of the transposon into the same DNA molecule. Immunity is conferred by the terminal inverted repeats of the transposon and is specific to each element of the family, indicating that the transposase TnpA is directly involved in the process.However, the molecular mechanism whereby this protein promotes efficient transposition into permissive targets while preventing transposition into immune targets remains unknown. Here, we demonstrate that both functions of TnpA can be uncoupled from each other by isolating and characterizing mutants that are proficient in transposition (T+) but impaired in immunity (I-). The identified T+/I- mutations are clustered into separate structural and functional domains of TnpA, indicating that different activities of the protein contribute to immunity.Combination of separate mutations had synergistic effects on target immunity but contrasting effects on transposition. One class of mutations was found to stimulate transposition, whereas other mutations appeared to reduce TnpA activity. The data are discussed with respect to alternative models in which TnpA acts as a specific determinant to both establish and respond to immunity. PMID:22624153

  6. The Sugar Creek zinc deposit, Jackson Co. TN -- Exploration history, geology and mineralization

    SciTech Connect

    Reinbold, G.; Moran, A.V.; Stevens, D.L. (Independence Mining Co. Inc., Reno, NV (United States))

    1993-03-01

    During the 60's and 70's zinc exploration of central TN and KY was active. The Sugar Creek Project was one of several investigated by Exxon. The discovery hole, Cu 15, was drilled in early 1973. The Sugar Creek Zinc Deposit was acquired by Independence Mining Co. in 1986 and I.M.C. has subsequently completed additional drilling, both stepout and confirmation holes. A total of 137 holes for 300,833 ft have been drilled. The Sugar Creek deposit is a typical Tennessee zinc deposit (Mississippi Valley Type) which occurs in solution collapse breccias in the Lower Ordovician, Knox Dolomite. The Knox consists of fine grained dolomite with interlayered limestones and crystalline dolomite. Only scattered residual limestone is found in the Sugar Creek area. Collapse breccias have formed which control zinc deposition and are similar to other TN Zn. deposits. At Sugar Creek the types of breccias include: a vertically exaggerated glory hole breakthrough breccia which extends to within 137 ft. of the Knox unconformity, has 500 ft. of zinc mineralization with 8 significant zinc intervals; holes with stacked zinc intervals interpreted to be sides of breakthrough breccia; and single zinc intervals in laterally positioned bedded mineral zones. A total of 99 holes were drilled in the more intense mineralized areas. The ratio of ore to non ore holes is nearly 1 to 1. The mineralization is typical M.V.T. with predominantly sphalerite and only minor occurrences of galena, fluorite, pyrite, etc.

  7. Establishment of an Arbitrary PCR for Rapid Identification of Tn917 Insertion Sites in Staphylococcus epidermidis: Characterization of Biofilm-Negative and Nonmucoid Mutants

    Microsoft Academic Search

    Johannes K.-M. Knobloch; Max Nedelmann; Kathrin Kiel; Katrin Bartscht; Matthias A. Horstkotte; Sabine Dobinsky; Holger Rohde; Dietrich Mack

    2003-01-01

    Transposon mutagenesis with the Enterococcus faecalis transposon Tn917 is a genetic approach frequently used to identify genes related with specific phenotypes in gram-positive bacteria. We established an arbitrary PCR for the rapid and easy identification of Tn917 insertion sites in Staphylococcus epidermidis with six independent, well-characterized biofilm-negative Tn917 transposon mutants, which were clustered in the icaADBC gene locus or harbor

  8. Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A 4, reacting with Tn (GalNA ?l ? Ser\\/Thr or galabiose (Gal ?1 ? 4Gal) containing ligands

    Microsoft Academic Search

    Shuh-Chyung Song; Elvin A. Kabat

    1996-01-01

    Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A4(GS I-A4) reacting with the Tn(GalNAc?1 ? Ser\\/Thr) sequence or human blood group Pk active dissacharide (E, Gal?1 ? 4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P1 or Tn active glycoproteins were tested by QPA, GS IA4 reacted strongly with both the Tn active glycoproteins purified from

  9. Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.

    PubMed

    Freire, Teresa; Zhang, Xiaoming; Dériaud, Edith; Ganneau, Christelle; Vichier-Guerre, Sophie; Azria, Elie; Launay, Odile; Lo-Man, Richard; Bay, Sylvie; Leclerc, Claude

    2010-11-01

    In vivo targeting of C-type lectin receptors is an effective strategy for increasing antigen uptake and presentation by dendritic cells (DCs). To induce efficient immune response, glycosylated tumor-associated Tn antigens were used to target DCs through binding to macrophage galactose-type lectin (MGL). The capacity of Tn-glycosylated antigens-and the multiple antigenic glycopeptide Tn3 therapeutic candidate vaccine-to target mouse and human MGL(+) DCs are demonstrated, especially regarding dermal DCs. In mice, MGL(+) CD103(-) dermal DCs efficiently captured and processed glycosylated Tn antigen in vivo, inducing a potent major histocompatibility complex (MHC) class II-restricted T-cell response. Intradermal immunization with Tn-glycopeptides induced high levels of Th2 cytokines-even in the presence of unmethylated cytosine-phosphate-guanosine-and was associated with increased expansion of the germinal center B-cell population. Therefore, MGL acts as an efficient endocytic antigen receptor on dermal DCs in vivo, able to prime Tn-specific T- and B-cell responses. Moreover, even in the absence of adjuvant, immunization with this glycosidic Tn-based vaccine induced high levels of anti-Tn antibody responses, recognizing human tumor cells. In vivo DC-targeting strategies, based on Tn-MGL interactions, constitute a promising strategy for enhancing antigen presentation and inducing potent antibody response. PMID:20720186

  10. Isolation and characterization of antibodies against three consecutive Tn-antigen clusters from a phage library displaying human single-chain variable fragments.

    PubMed

    Sakai, Keiko; Yuasa, Noriyuki; Tsukamoto, Kazuhiro; Takasaki-Matsumoto, Ayano; Yajima, Yukiko; Sato, Reiko; Kawakami, Hiroko; Mizuno, Mamoru; Takayanagi, Atsushi; Shimizu, Nobuyoshi; Nakata, Munehiro; Fujita-Yamaguchi, Yoko

    2010-06-01

    The Tn-antigen, GalNAcalpha-Ser/Thr, is a tumour-associated carbohydrate antigen that may provide a sensitive and specific marker for pre-clinical detection of carcinoma and a target for cancer therapies. We recently reported that MLS128 monoclonal antibody treatment significantly inhibited colon and breast cancer cell growth. On the basis of our observations, the present study aimed to produce human anti-Tn-antigen antibodies with specificity similar to that of MLS128 monoclonal antibody, which recognizes a structure of three consecutive Tn-antigens (Tn3). Six phage clones displaying human single-chain variable fragments (scFvs) were isolated from a newly constructed phage library by panning and screening with a synthetic Tn3-peptide. Deduced amino-acid sequences of six anti-Tn3 scFvs exhibited a high degree of homology. Of those, anti-Tn3 4E10 and 4G2 scFv proteins were successfully purified from phage-infected Escherichia coli to near homogeneity. Surface plasmon resonance analyses revealed a K(D) of purified scFv proteins for Tn3 on an order of 10(-7) M, which is high for carbohydrate-specific monovalent antibodies. Further analyses suggested that both scFv proteins also bind to Tn2 and cultured colon and breast cancer cells. These results demonstrated the potential for use of these scFvs in developing antibody therapeutics targeting colon and breast cancer. PMID:20147453

  11. Retrofit Savings for Brazos County

    E-print Network

    Baltazar-Cervantes, J. C.; Shao, X.; Claridge, D. E.

    2001-01-01

    &M University System BRAZOS COUNTY SumarySav2000m.xls 10/23/2001 BRAZOS COUNTY SumarySav2000(kW)m.xls 10/23/2001 Summary of energy savings from May 2000 to April 2001 Individual Reports BRAZOS COUNTY ENERGY SYSTEMS LABORATORY Texas Engineering Experiment Station... COUNTY ENERGY SYSTEMS LABORATORY Texas Engineering Experiment Station Texas A&M University System SAV-AGEXT JWCMMUUW W27/01 11:05 AM SAV-AGEXT 9/27/01 11:05 AM SAV-ARENAHALL 9/27/01 11:08 AM SAV-ARENAHALL 9/27/01 11:06 AM SAV-B2CENTER1 M7/01 1123 AM SAV...

  12. VERMONT COUNTY HEALTH DATA2

    EPA Science Inventory

    This datalayer contains Vermont Population and Health data describing public health (1986-2000), by county, extracted from various sources, such as; the Vermont Department of Health, the Vermont Center for Justice Research, the National Institute on Alcohol Abuse & Alcoholis...

  13. Sequencing and expression of the 6'-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae.

    PubMed

    Nobuta, K; Tolmasky, M E; Crosa, L M; Crosa, J H

    1988-08-01

    Plasmid-mediated amikacin resistance in Klebsiella pneumoniae resides on a 1.5-kilobase BamHI fragment which is part of the Tn3-related multiresistance transposon Tn1331. In this work, we present the complete nucleotide sequence of the amikacin resistance gene and the neighboring sequences. Maxicell experiments detected only one polypeptide of 23 kilodaltons, the product of one of the open reading frames identified as ORF I. Comparison of the complete sequence with that of Tn3 indicated that 396 base pairs located just upstream from ORF I are identical to a region between the end of the tnpR gene and the first six amino acids of the beta-lactamase transcript. Sequences which may act as hot spots for recombination were identified. One was located just after amino acid 6 of beta-lactamase, and the other was located at the end of the amikacin resistance gene. PMID:2841303

  14. 75 FR 25308 - Environmental Impact Statement: Winnebago County, IL and Rock County, WI

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ...Administration Environmental Impact Statement: Winnebago County, IL and Rock County, WI AGENCY: Federal Highway Administration (FHWA...Wisconsin Route 213 and Nye School Road northwest of Beloit, Rock County, Wisconsin to the interchange of Rockton Road and...

  15. Toole County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Infarction prevalence (Heart Attack) 4.4% 4.1% 6.0% All Sites Cancer 461.9 455.5 543.2 1 Community) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Toole County Secondary Data

  16. Hill County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 4.0% 4.1% 6.0% All Sites Cancer 461.9 (Region 2) 455.5 543.2 1 Community) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Hill County

  17. Lincoln County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 5.0% 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455.5 543.2 1 Community of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Lincoln County Secondary Data Analysis July 23

  18. Wibaux County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Infarction prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Wibaux County Secondary Data Analysis July 23, 2012 2

  19. Dawson County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Infarction prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Dawson County Secondary Data Analysis July 23

  20. Teton County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 4.4% 4.1% 6.0% All Sites Cancer 461.9 455.5 543.2 1 Community Health Data, MT County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Teton County Secondary Data Analysis July 23, 2012 2

  1. Phillips County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Myocardial Infarction prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Phillips County Secondary Data Analysis July 23

  2. Glacier County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 4.5% 4.1% 6.0% All Sites Cancer 461.9 (Region 2) 455.5 543.2 1 Community) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Glacier County

  3. Pondera County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 4.4% 4.1% 6.0% All Sites Cancer 461.9 455.5 543.2 1 Community Health Data, MT County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Pondera County Secondary Data Analysis

  4. Atlas of Historical County Boundaries

    NSDL National Science Digital Library

    So you'd like to know the boundaries of Washington's King County in the early 20th century? Or perhaps you'd like to know more about the shape of Nassau County in New York back in the 19th century? The Atlas of Historical County Boundaries can provide you such details. The project is housed at the William M. Scholl Center for American History and Culture at The Newberry Library in Chicago and it was completed in 2010. The Atlas includes such features as all boundary changes in states and countries, non-county areas, separate map or polygon files for every different county configuration, and other helpful features. Users can get started by clicking on different states of interest and just exploring as they see fit. It's also useful to read over the Using the Atlas area for additional assistance. Finally, visitors can also look at the Publications section for more information on the source material for these county boundaries.

  5. 75 FR 52367 - Notice of Inventory Completion: Memphis Pink Palace Museum, Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ...Roper referred to the Hatchie River bottoms in Lauderdale and Tipton counties as the `Big Hatchie Country' and I think that `mound' just got added on. Certainly the bulk of his stuff is from Morgan's Point/Bishop (40TP10).''...

  6. Revised TN Categorization for Colon Cancer Based on National Survival Outcomes Data

    PubMed Central

    Gunderson, Leonard L.; Jessup, John Milburn; Sargent, Daniel J.; Greene, Frederick L.; Stewart, Andrew K.

    2010-01-01

    Purpose The sixth edition of American Joint Committee on Cancer (AJCC) Cancer Staging Manual for colon cancer subdivided stage II into IIA (T3N0) and IIB (T4N0) and stage III into IIIA (T1-2N1M0), IIIB (T3-4N1M0), and IIIC (anyTN2M0). Subsequent analyses supported revised substaging of stage III because of improved survival for T1-2N2 versus T3-4N2 and T4N1 survival was more similar to T3-4N2 than to T3N1. The AJCC Hindgut Taskforce sought population-based validation that depth of invasion and nodal status interact to affect survival. Patients and Methods Surveillance, Epidemiology, and End Results (SEER) population-based data from January 1992 to December 2004 for 109,953 colon cancer patients were compared with National Cancer Data Base (NCDB) data on 134,206 patients. T4N0 cancers were stratified by tumors that perforate visceral peritoneum (T4a) versus tumors that invade or are adherent to adjacent organs or structures (T4b). N1 and N2 were stratified by number of involved positive lymph nodes (N+): N1a/N1b (1 v 2-3), N2a/N2b (4 to 6 v ? 7). Five-year observed and relative survival data were obtained for each TN category. Results SEER colon cancer analyses confirm that patients with T1-2N0 cancers have better survival than T3N0, T3N0 better than T4N0, T1-2N2 better than T3-4N2, and T4bN1 similar to T4N2. Patients with T4a lesions have better survival than T4b by N category. The number of positive nodes affects survival for each T category. Conclusion This SEER population-based colon cancer analysis is highly consistent with rectal cancer pooled analysis and SEER rectal cancer analyses, supporting the shift of T1-2N2 lesions from IIIC to IIIA/IIIB, shifting T4bN1 from IIIB to IIIC, subdividing T4/N1/N2, and revising substaging of stages II/III. Survival outcomes by TN category for colon and rectal cancer are strikingly similar. PMID:19949014

  7. Detachment faulting and its relationship to older structural events on Saddle Island, River Mountains, Clark County, Nevada

    Microsoft Academic Search

    Pierre Choukroune; Eugene I. Smith

    1985-01-01

    On Saddle Island a detachment fault of Tertiary age separates two structurally different plates. In the lower plate, Precambrian amphibolite and gneiss are strongly overprinted by a subhorizontal mylonitic foliation that may have formed during east-directed overthrusting of Sevier age. The mylonitic foliation is in turn overprinted by a later brittle deformation expressed by the detachment fault and by two

  8. Modular Evolution of TnGBSs, a New Family of Integrative and Conjugative Elements Associating Insertion Sequence Transposition, Plasmid Replication, and Conjugation for Their Spreading

    PubMed Central

    Guérillot, Romain; Da Cunha, Violette; Sauvage, Elisabeth; Bouchier, Christiane

    2013-01-01

    Integrative and conjugative elements (ICEs) have a major impact on gene flow and genome dynamics in bacteria. The ICEs TnGBS1 and TnGBS2, first identified in Streptococcus agalactiae, use a DDE transposase, unlike most characterized ICEs, which depend on a phage-like integrase for their mobility. Here we identified 56 additional TnGBS-related ICEs by systematic genome analysis. Interestingly, all except one are inserted in streptococcal genomes. Sequence comparison of the proteins conserved among these ICEs defined two subtypes related to TnGBS1 or TnGBS2. We showed that both types encode different conjugation modules: a type IV secretion system, a VirD4 coupling protein, and a relaxase and its cognate oriT site, shared with distinct lineages of conjugative elements of Firmicutes. Phylogenetic analysis suggested that TnGBSs evolved from two conjugative elements of different origins by the successive recruitment of a transposition module derived from insertion sequences (ISs). Furthermore, TnGBSs share replication modules with different plasmids. Mutational analyses and conjugation experiments showed that TnGBS1 and TnGBS2 combine replication and transposition upstream promoters for their transfer and stabilization. Despite an evolutionarily successful horizontal dissemination within the genus Streptococcus, these ICEs have a restricted host range. However, we reveal that for TnGBS1 and TnGBS2, this host restriction is not due to a transfer incompatibility linked to the conjugation machineries but most likely to their ability for transient maintenance through replication after their transfer. PMID:23435978

  9. Cointegrate-resolution of toluene-catabolic transposon Tn4651: determination of crossover site and the segment required for full resolution activity.

    PubMed

    Yano, Hirokazu; Genka, Hiroyuki; Ohtsubo, Yoshiyuki; Nagata, Yuji; Top, Eva M; Tsuda, Masataka

    2013-01-01

    Tn3-family transposon Tn4651 from Pseudomonas putida mt-2 plasmid pWW0 carries two divergently transcribed genes, tnpS and tnpT, for cointegrate-resolution. While tnpS encodes a tyrosine recombinase, tnpT encodes a protein that shows no homology to any other characterized protein. The Tn4651 resolution site was previously mapped within the 203-bp fragment that covered the tnpS and tnpT promoter region. To better understand the molecular mechanisms underlying the Tn4651 cointegrate-resolution, we determined the extent of the functional resolution site (designated the rst site) of Tn4651 and the location of the crossover site for the cointegrate-resolution. Deletion analysis of the rst region localized the fully functional rst site to a 136-bp segment. The analysis of the site-specific recombination between Tn4651 rst and a rst variant from the Tn4651-related transposon, Tn4661, indicated that the crossover occurs in the 33-bp inverted repeat region, which separates the 136-bp functional rst site into the tnpS- and tnpT-proximal segments. Electrophoretic mobility shift assays demonstrated specific binding of TnpT to the 20-bp inverted repeat region in the tnpT-proximal segment. The requirement for accessory sequences on both sides of the crossover site and the involvement of the unique DNA-binding protein TnpT suggest that the Tn4651-specified resolution system uses a different mechanism than other known resolution systems. Furthermore, comparative sequence analysis for Tn4651-related transposons revealed the occurrence of DNA exchange at the rst site among different transposons, suggesting an additional role of the TnpS-TnpT-rst system in the evolution of Tn4651-related transposons. PMID:22878084

  10. Visible-near infrared reflectance spectroscopy for estimating soil total nitrogen contents in the Sanjiang Yuan Regions, China: a case study of Yushu County and Maduo County, Qinghai province

    NASA Astrophysics Data System (ADS)

    Gao, Xiaohong; Yang, Yang; Zhang, Wei; Jia, Wei; Li, Jianshan; Tian, Chengming; Zhang, Yanjiao; He, Linhua

    2014-11-01

    The objective of this paper was to evaluate the prediction ability of visible and near-infrared reflectance spectroscopy(VNIRP) to estimate soil total nitrogen concentration (TN) in the Sanjiang Yuan regions, Qinghai province, China by using partial least squares regression (PLSR) method. A total of 149 soil samples (0-30cm) were collected respectively from Yushu county and Maduo county in August 2012. The soil total nitrogen concentrations were measured using Vario EL III elemental analyzer (Germany Elementar Inc), and the soil spectral reflectance of with spectral range 350-2500nm was measured using ASD FieldSpec 4 in the laboratory. PLSR model was used to relate the reflectance spectra and its five different pre-processing transformations including the first order derivative reflectance (FDR), the second order derivative reflectance(SDR), the logarithm of the inverse of the reflectance (Log (1/R)), the depth of the band (Band depth) and the first order derivative of the logarithm of reflectance (( Log R)') to the soil total nitrogen contents measured. The results showed that the combination of visible-near infrared reflectance spectroscopy and PLSR to estimate TN produced a good prediction ability with R2 < 0.76 and RPD < 2.01 in the Sanjiang Yuan regions. The best prediction ability was obtained by (Log R) ' with R2CV = 0.87, R2V =0.88 and RPD <2.67, followed by Log (1/R), Band Depth, SDR, FDR and Reflectance. Visible and near infrared spectroscopy provided a rapid and effective method for estimating soil TN from five different soil types in the Sanjiang Yuan Regions, Qinghai province.

  11. Five naturally bioactive molecules including two rhamnopyranoside derivatives isolated from the Streptomyces sp. strain TN58.

    PubMed

    Ben Ameur Mehdi, Raoudha; Shaaban, Khaled A; Rebai, Ines Karray; Smaoui, Slim; Bejar, Samir; Mellouli, Lotfi

    2009-01-01

    Extraction of 25 L fermentation broth of the newly isolated Streptomyces sp. strain TN58 and various separation and purification steps led to the isolation of five bioactive metabolites, namely brevianamide F (C1), reported from a streptomycete for the first time, N(beta)-acetyltryptamine (C2), thiazolidomycin (C3), and two rhamnopyranosides (C4 and C5). These two rhamnopyranosides were produced directly, without precursor addition. The chemical structure of these five active compounds was established on the basis of (1)H, (13)C/APT and 2D NMR spectra, ESI and EI-MS data, and by comparison with data from the literature. According to the biological studies, we show in this work that the compounds C1, C2, C4 and C5 possess antimicrobial activities. PMID:19662574

  12. TnBP?Triton X-45 Treatment of Plasma for Transfusion Efficiently Inactivates Hepatitis C Virus

    PubMed Central

    Chou, Ming-Li; Burnouf, Thierry; Chang, Shun-Pang; Hung, Ting-Chun; Lin, Chun-Ching; Richardson, Christopher D.; Lin, Liang-Tzung

    2015-01-01

    Risk of transmission of hepatitis C virus (HCV) by clinical plasma remains high in countries with a high prevalence of hepatitis C, justifying the implementation of viral inactivation treatments. In this study, we assessed the extent of inactivation of HCV during minipool solvent/detergent (SD; 1% TnBP / 1% Triton X-45) treatment of human plasma. Luciferase-tagged infectious cell culture-derived HCV (HCVcc) particles were used to spike human plasma prior to treatment by SD at 31 ± 0.5°C for 30 min. Samples were taken before and after SD treatment and filtered on a Sep-Pak Plus C18 cartridge to remove the SD agents. Risk of cytotoxicity was assessed by XTT cell viability assay. Viral infectivity was analyzed based on the luciferase signals, 50% tissue culture infectious dose viral titer, and immunofluorescence staining for HCV NS5A protein. Total protein, cholesterol, and triglyceride contents were determined before and after SD treatment and C18 cartridge filtration. Binding analysis, using patient-derived HCV clinical isolates, was also examined to validate the efficacy of the inactivation by SD. SD treatment effectively inactivated HCVcc within 30 min, as demonstrated by the baseline level of reporter signals, total loss of viral infectivity, and absence of viral protein NS5A. SD specifically targeted HCV particles to render them inactive, with essentially no effect on plasma protein content and hemostatic function. More importantly, the efficacy of the SD inactivation method was confirmed against various genotypes of patient-derived HCV clinical isolates and against HCVcc infection of primary human hepatocytes. Therefore, treatment by 1% TnBP / 1% Triton X-45 at 31°C is highly efficient to inactivate HCV in plasma for transfusion, showing its capacity to enhance the safety of therapeutic plasma products. We propose that the methodology used here to study HCV infectivity can be valuable in the validation of viral inactivation and removal processes of human plasma-derived products. PMID:25658612

  13. TnBP?Triton X-45 treatment of plasma for transfusion efficiently inactivates hepatitis C virus.

    PubMed

    Chou, Ming-Li; Burnouf, Thierry; Chang, Shun-Pang; Hung, Ting-Chun; Lin, Chun-Ching; Richardson, Christopher D; Lin, Liang-Tzung

    2015-01-01

    Risk of transmission of hepatitis C virus (HCV) by clinical plasma remains high in countries with a high prevalence of hepatitis C, justifying the implementation of viral inactivation treatments. In this study, we assessed the extent of inactivation of HCV during minipool solvent/detergent (SD; 1% TnBP / 1% Triton X-45) treatment of human plasma. Luciferase-tagged infectious cell culture-derived HCV (HCVcc) particles were used to spike human plasma prior to treatment by SD at 31 ± 0.5°C for 30 min. Samples were taken before and after SD treatment and filtered on a Sep-Pak Plus C18 cartridge to remove the SD agents. Risk of cytotoxicity was assessed by XTT cell viability assay. Viral infectivity was analyzed based on the luciferase signals, 50% tissue culture infectious dose viral titer, and immunofluorescence staining for HCV NS5A protein. Total protein, cholesterol, and triglyceride contents were determined before and after SD treatment and C18 cartridge filtration. Binding analysis, using patient-derived HCV clinical isolates, was also examined to validate the efficacy of the inactivation by SD. SD treatment effectively inactivated HCVcc within 30 min, as demonstrated by the baseline level of reporter signals, total loss of viral infectivity, and absence of viral protein NS5A. SD specifically targeted HCV particles to render them inactive, with essentially no effect on plasma protein content and hemostatic function. More importantly, the efficacy of the SD inactivation method was confirmed against various genotypes of patient-derived HCV clinical isolates and against HCVcc infection of primary human hepatocytes. Therefore, treatment by 1% TnBP / 1% Triton X-45 at 31°C is highly efficient to inactivate HCV in plasma for transfusion, showing its capacity to enhance the safety of therapeutic plasma products. We propose that the methodology used here to study HCV infectivity can be valuable in the validation of viral inactivation and removal processes of human plasma-derived products. PMID:25658612

  14. TN-68 Spent Fuel Transport Cask Analytical Evaluation for Drop Events

    SciTech Connect

    Shah, M. J.; Klymyshyn, Nicholas A.; Adkins, Harold E.; Koeppel, Brian J.

    2007-03-30

    The U.S. Nuclear Regulatory Commission (NRC) is responsible for licensing commercial spent nuclear fuel transported in casks certified by NRC under the Code of Federal Regulations (10 CFR), Title 10, Part 71 [1]. Both the International Atomic Energy Agency regulations for transporting radioactive materials [2, paragraph 727], and 10 CFR 71.73 require casks to be evaluated for hypothetical accident conditions, which includes a 9-meter (m) (30-ft) drop-impact event onto a flat, essentially unyielding, horizontal surface, in the most damaging orientation. This paper examines the behavior of one of the NRC certified transportation casks, the TN-68 [3], for drop-impact events. The specific area examined is the behavior of the bolted connections in the cask body and the closure lid, which are significantly loaded during the hypothetical drop-impact event. Analytical work to evaluate the NRC-certified TN-68 spent fuel transport cask [3] for a 9-m (30-ft) drop-impact event on a flat, unyielding, horizontal surface, was performed using the ANSYS® [4] and LS DYNA™ [5] finite-element analysis codes. The models were sufficiently detailed, in the areas of bolt closure interfaces and containment boundaries, to evaluate the structural integrity of the bolted connections under 9-m (30-ft) free-drop hypothetical accident conditions, as specified in 10 CFR 71.73. Evaluation of the cask for puncture, caused by a free drop through a distance of 1-m (40-in.) onto a mild steel bar mounted on a flat, essentially unyielding, horizontal surface, required by 10 CFR 71.73, was not included in the current work, and will have to be addressed in the future. Based on the analyses performed to date, it is concluded that, even though brief separation of the flange and the lid surfaces may occur under some conditions, the seals would close at the end of the drop events, because the materials remain elastic during the duration of the event.

  15. Population substructure in Cache County, Utah: the Cache County study

    PubMed Central

    2014-01-01

    Background Population stratification is a key concern for genetic association analyses. In addition, extreme homogeneity of ethnic origins of a population can make it difficult to interpret how genetic associations in that population may translate into other populations. Here we have evaluated the genetic substructure of samples from the Cache County study relative to the HapMap Reference populations and data from the Alzheimer's Disease Neuroimaging Initiative (ADNI). Results Our findings show that the Cache County study is similar in ethnic diversity to the self-reported "Whites" in the ADNI sample and less homogenous than the HapMap CEU population. Conclusions We conclude that the Cache County study is genetically representative of the general European American population in the USA and is an appropriate population for conducting broadly applicable genetic studies. PMID:25078123

  16. 313 Conference Center, Knoxville, TN 37996-4137 865-974-0150 Kids U Parental Consent Form

    E-print Network

    Wang, Xiaorui "Ray"

    in activities related to Kids U. Furthermore, we (I) are the parent(s) or legal guardian(s) of this participant313 Conference Center, Knoxville, TN 37996-4137 · 865-974-0150 Kids U Parental Consent Form Please and hereby grant permission for him/her to participate fully in Kids U 2013, and hereby give permission

  17. RADIOCARBON MEASUREMENT OF THE BIOGENIC CONTRIBUTION TO SUMMERTIME PM 2.5 AMBIENT AEROSOL IN NASHVILLE, TN

    EPA Science Inventory

    Radiocarbon (14C) measurements performed on PM-2.5 samples collected near Nashville, TN from June 21 to July 13, 1999, showed high levels of modern carbon, ranging from 56 to 80% of the total carbon in the samples. Radiocarbon measurements performed on dichloromethane extracts of...

  18. Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis

    PubMed Central

    Seier-Petersen, M. A.; Jasni, A.; Aarestrup, F. M.; Vigre, H.; Mullany, P.; Roberts, A. P.; Agersø, Y.

    2014-01-01

    Objectives Large amounts of biocides are used to reduce and control bacterial growth in the healthcare sector, food production and agriculture. This work explores the effect of subinhibitory concentrations of four commonly used biocides (ethanol, hydrogen peroxide, chlorhexidine digluconate and sodium hypochlorite) on the conjugative transposition of the mobile genetic element Tn916. Methods Conjugation assays were carried out between Bacillus subtilis strains. The donor containing Tn916 was pre-exposed to subinhibitory concentrations of each biocide for a defined length of time, which was determined by an analysis of the transcriptional response of the promoter upstream of tet(M) using ?-glucuronidase reporter assays. Results Ethanol significantly (P?=?0.01) increased the transfer of Tn916 by 5-fold, whereas hydrogen peroxide, chlorhexidine digluconate and sodium hypochlorite did not significantly affect the transfer frequency. Conclusions These results suggest that exposure to subinhibitory concentrations of ethanol may induce the transfer of Tn916-like elements and any resistance genes they contain. PMID:24092655

  19. BART goes multilingual: The UniTN / Essex submission to the CoNLL-2012 Shared Task

    E-print Network

    Moschitti, Alessandro

    ). They present a challeng- ing problem: the systems are required to pro- vide entity mention detection (EMD) and design a proper coreference resolver for both languages. At UniTN/Essex, we have focused on these parts corpus, such heuristics can be adjusted in a straightforward way. Creating a robust rule-based EMD module

  20. loredo@spacenet.tn.cornell.edu http://www.astro.cornell.edu/staff/loredo/bayes/ Outline Probability

    E-print Network

    Loredo, Thomas J.

    loredo@spacenet.tn.cornell.edu http://www.astro.cornell.edu/staff/loredo/bayes/ Outline #15; Parametric uncertainty Bernoulli, Laplace, Bayes, Gauss: : : p(x) describes how probability (plausibility) is distributed mathematically (large number theorems, Bayes's theorem). Frequentist: Probability describes \\randomness" Venn

  1. Minimization of the Escherichia coli genome using a Tn5-targeted Cre\\/loxP excision system

    Microsoft Academic Search

    Byung Jo Yu; Bong Hyun Sung; Michael D. Koob; Choong Hoon Lee; Jun Hyoung Lee; Won Sik Lee; Mi Sun Kim; Sun Chang Kim

    2002-01-01

    An increasing number of microbial genomes have been completely sequenced, and functional analyses of these genomic sequences are under way. To facilitate these analyses, we have developed a genome-engineering tool for determining essential genes and minimizing bacterial genomes. We made two large pools of independent transposon mutants in Escherichia coli using modified Tn5 transposons with two different selection markers and

  2. ION GPS '99, 14-17 September 1999, Nashville, TN Testing a GPS-based System for the Support of

    E-print Network

    Santerre, Rock

    ION GPS '99, 14-17 September 1999, Nashville, TN Testing a GPS-based System for the Support of Ship as research professional on the GPS OTF project within the GEOIDE Network of Centres of Excellence of Geomatic Sciences at Laval University, where he conducts research and teaching in precise GPS static

  3. Flexible Photoaligned Permanent Bistable TN-LCD Xihua Li, Fion S. Y. Yeung, V. G. Chigrinov, and H. S. Kwok

    E-print Network

    Flexible Photoaligned Permanent Bistable TN-LCD Xihua Li, Fion S. Y. Yeung, V. G. Chigrinov, and H (LCD) in many applications such as mobile phones, smart cards, electronic newspaper and Integrated display 1 . Compared to traditional glass LCD's, flexible displays have many advantages like being thin

  4. Mobility and Generation of Mosaic Non-Autonomous Transposons by Tn3-Derived Inverted-Repeat Miniature Elements (TIMEs)

    PubMed Central

    Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

    2014-01-01

    Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons – Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element “captured” with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not only of transposons and plasmids, but also of other types of mobile genetic elements. PMID:25121765

  5. Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs).

    PubMed

    Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

    2014-01-01

    Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons--Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element "captured" with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not only of transposons and plasmids, but also of other types of mobile genetic elements. PMID:25121765

  6. A rapid and simple method for the isolation of mutant variants regulating tissue-specific expression of the TnI gene through drug selection

    SciTech Connect

    Lee, Youngwon; Kim, Myoung Hee [Korea Research Institute of Bioscience and Biotechnology, Taejon (Korea, Republic of); Emerson, C.P. Jr. [Fox Chase Cancer Center, Philadelphia, PA (United States)

    1995-12-01

    TnINEO fusion gene was constructed by fusing 3.4-kbp of quail TnI genomic DNA sequences spanning the promoter to exon 5 and a neo gene in frame. A myoblast cell line was established after transfection of pTnINEO. Since this cell line was passaged several times, a high frequency of neomycin (G418) sensitivity conversion was detected. Two drug-resistant variants were analyzed through genomic Southern blot and S1 nuclease protection assay. One variant has a mutation(s) in the regulatory element that activated the dormant TnI promoter-enhancer in myoblast, and the other has shown the geonomic rearrangement. This result presented the possibility of isolating factor(s) that activate the muscle-specific TnI promoter simply by screening drug-resistant cells having appropriate mutations. 12 refs., 4 fig.

  7. Robust rat pulmonary radioprotection by a lipophilic Mn N-alkylpyridylporphyrin, MnTnHex-2-PyP5+?

    PubMed Central

    Gauter-Fleckenstein, Benjamin; Reboucas, Julio S.; Fleckenstein, Katharina; Tovmasyan, Artak; Owzar, Kouros; Jiang, Chen; Batinic-Haberle, Ines; Vujaskovic, Zeljko

    2014-01-01

    With the goal to enhance the distribution of cationic Mn porphyrins within mitochondria, the lipophilic Mn(III)meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin, MnTnHex-2-PyP5+ has been synthesized and tested in several different model of diseases, where it shows remarkable efficacy at as low as 50 µg/kg single or multiple doses. Yet, in a rat lung radioprotection study, at higher 0.6–1 mg/kg doses, due to its high accumulation and micellar character, it became toxic. To avoid the toxicity, herein the pulmonary radioprotection of MnTnHex-2-PyP5+ was assessed at 50 µg/kg. Fischer rats were irradiated to their right hemithorax (28 Gy) and treated with 0.05 mg/kg/day of MnTnHex-2-PyP5+ for 2 weeks by subcutaneously-implanted osmotic pumps, starting at 2 h post-radiation. The body weights and breathing frequencies were followed for 10 weeks post-radiation, when the histopathology and immunohistochemistry were assessed. Impact of MnTnHex-2-PyP5+ on macrophage recruitment (ED-1), DNA oxidative damage (8-OHdG), TGF-?1, VEGF(A) and HIF-1? were measured. MnTnHex-2-PyP5+ significantly decreased radiation-induced lung histopathological (H&E staining) and functional damage (breathing frequencies), suppressed oxidative stress directly (8-OHdG), or indirectly, affecting TGF-?1, VEGF (A) and HIF-1? pathways. The magnitude of the therapeutic effects is similar to the effects demonstrated under same experimental conditions with 120-fold higher dose of ~5000-fold less lipophilic Mn(III)meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin, MnTE-2-PyP5+. PMID:24624330

  8. Robust rat pulmonary radioprotection by a lipophilic Mn N-alkylpyridylporphyrin, MnTnHex-2-PyP(5+).

    PubMed

    Gauter-Fleckenstein, Benjamin; Reboucas, Julio S; Fleckenstein, Katharina; Tovmasyan, Artak; Owzar, Kouros; Jiang, Chen; Batinic-Haberle, Ines; Vujaskovic, Zeljko

    2014-01-01

    With the goal to enhance the distribution of cationic Mn porphyrins within mitochondria, the lipophilic Mn(III)meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin, MnTnHex-2-PyP(5+) has been synthesized and tested in several different model of diseases, where it shows remarkable efficacy at as low as 50 µg/kg single or multiple doses. Yet, in a rat lung radioprotection study, at higher 0.6-1 mg/kg doses, due to its high accumulation and micellar character, it became toxic. To avoid the toxicity, herein the pulmonary radioprotection of MnTnHex-2-PyP(5+) was assessed at 50 µg/kg. Fischer rats were irradiated to their right hemithorax (28 Gy) and treated with 0.05 mg/kg/day of MnTnHex-2-PyP(5+) for 2 weeks by subcutaneously-implanted osmotic pumps, starting at 2 h post-radiation. The body weights and breathing frequencies were followed for 10 weeks post-radiation, when the histopathology and immunohistochemistry were assessed. Impact of MnTnHex-2-PyP(5+) on macrophage recruitment (ED-1), DNA oxidative damage (8-OHdG), TGF-?1, VEGF(A) and HIF-1? were measured. MnTnHex-2-PyP(5+) significantly decreased radiation-induced lung histopathological (H&E staining) and functional damage (breathing frequencies), suppressed oxidative stress directly (8-OHdG), or indirectly, affecting TGF-?1, VEGF (A) and HIF-1? pathways. The magnitude of the therapeutic effects is similar to the effects demonstrated under same experimental conditions with 120-fold higher dose of ~5000-fold less lipophilic Mn(III)meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin, MnTE-2-PyP(5+). PMID:24624330

  9. Cooperative regulation of myosin-S1 binding to actin filaments by a continuous flexible Tm-Tn chain.

    PubMed

    Mijailovich, Srboljub M; Kayser-Herold, Oliver; Li, Xiaochuan; Griffiths, Hugh; Geeves, Michael A

    2012-12-01

    The regulation of striated muscle contraction involves cooperative interactions between actin filaments, myosin-S1 (S1), tropomyosin (Tm), troponin (Tn), and calcium. These interactions are modeled by treating overlapping tropomyosins as a continuous flexible chain (CFC), weakly confined by electrostatic interactions with actin. The CFC is displaced locally in opposite directions on the actin surface by the binding of either S1 or Troponin I (TnI) to actin. The apparent rate constants for myosin and TnI binding to and detachment from actin are then intrinsically coupled via the CFC model to the presence of neighboring bound S1s and TnIs. Monte Carlo simulations at prescribed values of the CFC stiffness, the CFC's degree of azimuthal confinement, and the angular displacements caused by the bound proteins were able to predict the stopped-flow transients of S1 binding to regulated F-actin. The transients collected over a large range of calcium concentrations could be well described by adjusting a single calcium-dependent parameter, the rate constant of TnI detachment from actin, k(-I). The resulting equilibrium constant K(B) ? 1/K(I) varied sigmoidally with the free calcium, increasing from 0.12 at low calcium (pCa >7) to 12 at high calcium (pCa <5.5) with a Hill coefficient of ~2.15. The similarity of the curves for excess-actin and excess-myosin data confirms their allosteric relationship. The spatially explicit calculations confirmed variable sizes for the cooperative units and clustering of bound myosins at low calcium concentrations. Moreover, inclusion of negative cooperativity between myosin units predicted the observed slowing of myosin binding at excess-myosin concentrations. PMID:23052974

  10. Mouse-human chimeric anti-Tn IgG1 induced anti-tumor activity against Jurkat cells in vitro and in vivo.

    PubMed

    Ando, Hiroshi; Matsushita, Takefumi; Wakitani, Masako; Sato, Takashi; Kodama-Nishida, Sachiko; Shibata, Kenji; Shitara, Kenya; Ohta, So

    2008-09-01

    Tn-antigen (alpha-N-acetyl-galactosamine(GalNAc)-Ser/Thr) is a cancer-associated carbohydrate antigen expressed in various epithelial and hematological cancers, and although a number of anti-Tn IgG and IgM antibodies have been generated, they have not been fully validated for cancer immunotherapy. In this study, we generated a novel murine anti-Tn IgG1 monoclonal antibody, KM3413, by immunization of mucins purified from a culture supernatant of LS180: a human colon cancer cell line. The binding of KM3413 was detected against consecutive Tn-antigens (Tn3 and Tn2), but not against monovalent antigens (Tn1). The affinity (K(D)) of KM3413 was determined to be about 10(-7) M with BIAcore. Cross-reactivity against type-A blood antigen, which shares a sugar residue, alpha-linked GalNAc, with Tn-antigen, was not detected. Next, we generated mouse-human chimeric IgG1 of KM3413 (cKM3413) and evaluated its anti-tumor activities against Jurkat: a human T-lymphoid leukemia cell line. In vitro assay revealed that cKM3413 induced antibody-dependent cellular cytotoxicity (ADCC) and direct killing activity with cross-link antibody. Furthermore, treatment of cKM3413 (1 or 10 mg/kg) showed significantly better survival of Jurkat-inoculated C.B-17/lcr-scid Jcl mice compared with controls using PBS treatment (p<0.001). These results suggest that humanized antibody against clustered Tn-antigens is a promising therapeutic antibody against Tn-positive cancers. PMID:18758069

  11. Monoclonal antibodies toward different Tn-amino acid backbones display distinct recognition patterns on human cancer cells. Implications for effective immuno-targeting of cancer.

    PubMed

    Mazal, Daniel; Lo-Man, Richard; Bay, Sylvie; Pritsch, Otto; Dériaud, Edith; Ganneau, Christelle; Medeiros, Andrea; Ubillos, Luis; Obal, Gonzalo; Berois, Nora; Bollati-Fogolin, Mariela; Leclerc, Claude; Osinaga, Eduardo

    2013-06-01

    The Tn antigen (GalNAc?-O-Ser/Thr) is a well-established tumor-associated marker which represents a good target for the design of anti-tumor vaccines. Several studies have established that the binding of some anti-Tn antibodies could be affected by the density of Tn determinant or/and by the amino acid residues neighboring O-glycosylation sites. In the present study, using synthetic Tn-based vaccines, we have generated a panel of anti-Tn monoclonal antibodies. Analysis of their binding to various synthetic glycopeptides, modifying the amino acid carrier of the GalNAc(*) (Ser* vs Thr*), showed subtle differences in their fine specificities. We found that the recognition of these glycopeptides by some of these MAbs was strongly affected by the Tn backbone, such as a S*S*S* specific MAb (15G9) which failed to recognize a S*T*T* or a T*T*T* structure. Different binding patterns of these antibodies were also observed in FACS and Western blot analysis using three human cancer cell lines (MCF-7, LS174T and Jurkat). Importantly, an immunohistochemical analysis of human tumors (72 breast cancer and 44 colon cancer) showed the existence of different recognition profiles among the five antibodies evaluated, demonstrating that the aglyconic part of the Tn structure (Ser vs Thr) plays a key role in the anti-Tn specificity for breast and colon cancer detection. This new structural feature of the Tn antigen could be of important clinical value, notably due to the increasing interest of this antigen in anticancer vaccine design as well as for the development of anti-Tn antibodies for in vivo diagnostic and therapeutic strategies. PMID:23604173

  12. The vascular flora of Madison County, Texas 

    E-print Network

    Neill, Amanda Kathryn

    2000-01-01

    Vascular plants were collected in Madison County, Texas from 1996-2000 to create a vouchered record of species occurance, abundance, and habitat preference. Plants were collected from 233 sites in the county that are ...

  13. libertyCounty Number of volunteers 50

    E-print Network

    Ma, Lena

    Ension: Early childhood training, Leadership development, Food nutrition and health programs, Life skill development for 4-H youth Giving Recent donors residing in county 50 FY 2012 donors residing in county 19

  14. Burleson County State of the Community Report

    E-print Network

    Doremus, Lindsay; De Oliveira, Thiago Coelho; Simcic, Lauren

    2015-01-01

    Burleson County, Texas State of the Community Report Lindsay Doremus, Thiago Coelho De Oliveira, Lauren Simcic Doremus. Oliveira. Simcic. Table of Contents INTRODUCTION... ............................................................................................................ 13 Burleson County ........................................................................................................................... 13 Residential Use...

  15. 76 FR 8808 - Supplemental Final Environmental Impact Statement: Clark County, Indiana, and Jefferson County, KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ...Clark County, Indiana, and Jefferson County, KY AGENCY: Federal...Clark County, Indiana and Jefferson County, Kentucky. FOR FURTHER...INFORMATION CONTACT: Mr. Duane Thomas, Project Manager, Federal...223-6720, e-mail: Duane.Thomas@dot.gov or Mr. Gary...

  16. Annual St. Lucie County's Southern Swine 4-H Club

    E-print Network

    Florida, University of

    2nd Annual St. Lucie County's Southern Swine 4-H Club and Indian River County Landfill Hog National Association of Counties (NACo) 2nd Annual St. Lucie County's Southern Swine 4-H Club Hog Challenge & Indian River Counties. Proceeds to benefit St. Lucie County's Southern Swine 4-H's Club's' "Behind

  17. Origin and structural implications of upper Miocene rhyolites in Kingston Canyon, Piute County, Utah.

    USGS Publications Warehouse

    Rowley, P.D.; Steven, T.A.; Mehnert, H.H.

    1981-01-01

    Kingston Canyon is one of the deepest antecedent canyons in the High Plateaus subprovince of the Colorado Plateaus. Here the E Fork of the Sevier River flows westward transversely across the gently E tilted Sevier Plateau, which is developed on a basin-range fault block uplifted more than 1500m along the Sevier fault zone on the W. Upper Tertiary rhyolites, uncommon in SW Utah, occur both on the northern rim and in the bottom of Kingston Canyon. Those on the northern rim consist of lava flows and volcanic domes of the rhyolite of Forshea Mountain, dated by K/Ar methods at 7.6Ma old. Those in the bottom of Kingston Canyon, the rhyolite of Phonolite Hill, are especially well exposed and provide spectacular examples of a pyroclastic cone whose base is about at river level and a steep-sided volcanic dome emplaced into and through these deposits. The pyroclastic deposits, formerly 500 or more metres thick, consist of airfall, mudflow, and ash-flow(?) material of rhyolite and foreign lithic fragments especially olivine basalt. The dome consists of flow-banded, mostly devitrified rhyolite as much as 500m thick; it has been dated by K/Ar methods at 5.4Ma. In addition to the rhyolites, a dome and lava-flow complex, the rhyodacite of Dry Lake, occurs near the northern rim and is considered to postdate the rhyolite of Forshea Mountain and predate the rhyolite of Phonolite Hill. -from Authors

  18. Presidio County State of the Community Reprort 

    E-print Network

    Xiao, Dong; Hwang, Jinuk; McCharen, Rob

    2015-01-01

    STATE OF THE COMMUNITY REPORT PRESIDIO COUNTY, TEXAS Dong Xiao|Jinuk Hwang|Rob McCharen Department of Landscape Architecture & Urban Planning State of the Community Report Presidio County, TX 1 Executive...PRESIDIO MARFA PRESIDIO COUNTY, TEXAS State of the Community Population Economy Housing Environment SWOT Analysis Department of Landscape Architecture & Urban Planning Jinuk Hwang | Robert McCharen | Dong Xiao Presidio County, TX Located...

  19. Burleson County State of the Community Report

    E-print Network

    Jiang, Ying; Thapa, Jeewasmi; Safarova, Bara

    2015-01-01

    STATE OF THE COMMUNITY REPORT BURLESON COUNTY, TEXAS Prepared by: Ying Jiang Jeewasmi Thapa Bara Safarova   State of the Community Report: Burleson County Ying Jiang | Jeewasmi Thapa | Bara Safarova 1... Composition 2.6 ?Population Projections 3. ?Economic analysis 3.1 ?Majority of population employed outside of the county 3.2 ?Major employers by industry sector 3.3 ?Burleson County industry sectors comparison with Texas benchmark 3.4 ?Basic sectors...

  20. Liberty County State of the Community

    E-print Network

    Tedford, Emily; Gao, Fan; Reja, Md Yousuf

    2015-01-01

    LIBERTY COUNTY State of the Community Emily Tedford Fan Gao Md Yousuf Reja 1 INTRODUCTION Liberty County, Texas is located in southeastern Texas and part of the Houston... metropolitan area. The County is bisected by the Trinity River from North to South and US Highway 90 from East to West. The County has a very strategic location, along the Trinity River, allowing access to the Port to the South, and along US Highway 90...

  1. Trouble Brewing in Orange County. Policy Brief

    ERIC Educational Resources Information Center

    Buck, Stuart

    2010-01-01

    Orange County will soon face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that Orange County faces a total $41.2 billion liability for retiree benefits that are underfunded--including $9.4 billion for the county pension system and an estimated…

  2. Lee County Government Division of Natural Resources

    E-print Network

    Demers, Nora Egan

    County that also rely directly good water quality... Fisheries, Real Estate, etc... #12;Caloosahatchee Resources #12;·Ecosystem Services - Lee County's Focus ·Lee County's Water Quality Based Economy & Impacts) or industrial (such as milling) processes or transportation, urban water supply. Water supply Storage

  3. Johnson County Business and Industry Training Needs.

    ERIC Educational Resources Information Center

    Johnson County Community Coll., Overland Park, KS. Office of Institutional Research.

    In fall 1982, Johnson County Community College (JCCC) conducted two surveys to determine the internal training needs of business and industry in Johnson County. One survey was mailed to the chief executives of some 2,000 businesses and industries, and another was distributed to about 2,000 employees of a large corporation in the county. Study…

  4. Tennessee Higher Education County Profiles, 2009

    ERIC Educational Resources Information Center

    Tennessee Higher Education Commission, 2009

    2009-01-01

    This report presents a localized perspective on Tennessee higher education for the year 2009, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; and (4) number of county residents participating in the state's primary need-based and merit-based financial aid…

  5. Once Distressed, Jackson County Moves On.

    ERIC Educational Resources Information Center

    Baldwin, Fred D.

    1998-01-01

    In 1981, Jackson County, North Carolina, appeared on the first list of "distressed counties" in Appalachia. Since then, the rural county has made significant improvements by investing in its physical infrastructure (which also promotes tourism); fostering economic-development partnerships among governments, small businesses, and local colleges;…

  6. Community Types and Mortality in Georgia Counties

    ERIC Educational Resources Information Center

    Young, Frank W.

    2012-01-01

    Using an "ecological regional analysis" methodology for defining types of communities and their associated mortality rates, this study of Georgia's 159 counties finds that the suburban and town centered counties have low mortality while the city-centered type predicts low mortality for the whites. The military-centered counties do not predict. The…

  7. Kinship Care in Walton County.

    ERIC Educational Resources Information Center

    Charon, Sara L.; Nackerud, Larry

    1996-01-01

    The quality of life of maltreated children placed with relatives was examined through interviews with nine families in Walton County (Georgia) who had taken in related children. Over half of the children had experienced some improvement in home life, school performance, and their physical and mental status. Kinship care families indicated needs…

  8. Garfield County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community Health Data, MT. Cancer 2. Heart Disease 3. Pneumonia, CLRD*, Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Garfield County Secondary Data Analysis July 23

  9. Valley County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Infarction prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. Diabetes 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Valley County Secondary Data Analysis July 23, 2012 2

  10. Prairie County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community Health Data, MT. Heart Disease, Cancer 2. CLRD* 3. Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Prairie County Secondary Data Analysis July 23, 2012 2

  11. Mineral County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 4.0% 4.1% 6.0% All Sites Cancer 466.5 455.5 543.2 1 Community Health Data, MT2 1. Cancer 2. Heart Disease 3. Unintentional Injuries**, CLRD*, Cerebrovascular Disease 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Mineral County

  12. Gallatin County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 2.4% 4.1% 6.0% All Sites Cancer 416.6 (Region 4) 455.5 543.2 1 Community Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3

  13. Sanders County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Infarction prevalence (Heart Attack) 4.0% (Region 5) 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455 Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3

  14. Missoula County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Myocardial Infarction prevalence (Heart Attack) 3.5% 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455 Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12

  15. Composting in Prince William County

    SciTech Connect

    Lyons, K.

    1995-10-01

    Hidden in a small industrial corner of Prince Williams County, in Northern Virginia, a composting facility, after its first flourishing year in business, has found itself part of a symbiotic triangle. Along with a landfill and a waste-to-energy (WTE) plant, the composting facility is one of three programs that make up a joint public-private venture and form a interjurisdictional solid waste/refuse exchange agreement. Faced with the prospects of having to close a landfill at the end of the year, a mandate on yard waste collection that was increasing collection tonnage, and no room for further landfill development, Fairfax County, with approximately 900,000 residents, needed help. In a turn-around situation, Prince William County--with approximately 240,000 residents, a low budget, and much space available for development--responded. Together the counties created a solid waste exchange. The basis for the program is a unity between the local governments on some solid waste issues and a composting facility. Composting, specifically yard waste composting, has been among the fastest-growing aspects of waste management. In 1990, the nation was composting 2% of its solid waste. By the end of 1995, according to the US EPA, between 4% and 7% of solid waste will be recovered through composting. The number of yard waste composting facilities operating has increased from 651 in 1988 to more than 3,000 in 1994.

  16. Yellowstone County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    36.5% 37.8% No Leisure time for physical activity1 20.9% 20.7% 0 5 10 15 20 25 30 35 Myocardial Infarction prevalence (Heart Attack) 4.1% 4.1% 6.0% All Sites Cancer 510.8 (Region 3) 455, Yellowstone, Big Horn, and Carbon Chronic Disease Hospitalization Rates County Montana Stroke1 Per

  17. pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

    SciTech Connect

    Matsumoto, Yasuyuki; Zhang, Qing [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Akita, Kaoru; Nakada, Hiroshi [Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-ku, Kyoto 603-8555 (Japan)] [Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-ku, Kyoto 603-8555 (Japan); Hamamura, Kazunori; Tokuda, Noriyo [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Tsuchida, Akiko [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan) [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Noguchi Institute, 1-8-1 Kaga, Itabashi, Tokyo 173-0003 (Japan); Matsubara, Takeshi; Hori, Tomoko; Okajima, Tetsuya [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Furukawa, Keiko [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan) [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, 1200 Matsumoto-cho, Kasugai 487-8501 (Japan); Urano, Takeshi [Department of Biochemistry, Shimane University School of Medicine, Izumo 693-8501 (Japan)] [Department of Biochemistry, Shimane University School of Medicine, Izumo 693-8501 (Japan); Furukawa, Koichi, E-mail: koichi@med.nagoya-u.ac.jp [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer ppGalNAc-T13 was up-regulated in high metastatic sublines of Lewis lung cancer. Black-Right-Pointing-Pointer ppGalNAc-T13 expression enhanced cell invasion activity in low metastatic sublines. Black-Right-Pointing-Pointer Trimeric Tn antigen was induced in the transfectant cells of ppGalNAc-T13 cDNA. Black-Right-Pointing-Pointer A major protein carrying trimeric Tn structure was identified as Syndecan-1. Black-Right-Pointing-Pointer Silencing of ppGalNAc-T13 resulted in the reduction of invasion and of metastasis.. -- Abstract: In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. These sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated by subcutaneous injection of T13-KD clones showed lower coalescence to fascia and peritoneum, and significantly reduced lung metastasis than control clones. These data suggested that high expression of pp-GalNAc-T13 gene generated trimeric Tn antigen on Syndecan-1, leading to the enhanced metastasis.

  18. Effects of MnTnHex-2-PyP on lung antioxidant defence system in asthma mice model.

    PubMed

    Dancheva, Violeta; Terziev, Lyudmil; Shopova, Veneta; Stavreva, Galya

    2012-12-01

    We aimed to study the MnTnHex-2-PyP effect on some markers of lung antioxidant defence system in mice asthma model.The study was carried out on 28 C57B1/6 mice divided into four treatment groups: group 1 - controls; group 2 - injected and inhaled with ovalbumin; group 3 - treated with MnTnHex-2-PyP and inhaled with phosphate buffered saline; group 4 - injected with ovalbumin and MnTnHex-2-PyP but also inhaled with ovalbumin. On days 24, 25 and 26, mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 ?l PBS, and those from groups 3 and 4 received a 100 ?l MnTnHex-2-PyP solution in PBS, ?ontaining 0,05mg/kg. The animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate. The activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. Ovalbumin decreased the activities of superoxide dismutase (p=0.01), catalase (p=0.002), glutathione peroxidase and non-protein sulphhydryl groups content (p<0.001) in comparison to controls. In group 4 (ovalbumin and MnTnHex-2-PyP) the activities of superoxide dismutase (p=0.044), catalase (p=0.045), glutathione peroxidase (p=0.002), and the non-protein sulphhydryl groups content (p<0.001) were significantly increased compared to ovalbumin (group 2).MnTnHex-2-PyP restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization. PMID:23264410

  19. Construction and expression of anti-Tn-antigen-specific single-chain antibody genes from hybridoma producing MLS128 monoclonal antibody.

    PubMed

    Yuasa, Noriyuki; Ogawa, Haruhiko; Koizumi, Tomoyuki; Tsukamoto, Kazuhiro; Matsumoto-Takasaki, Ayano; Asanuma, Hideki; Nakada, Hiroshi; Fujita-Yamaguchi, Yoko

    2012-04-01

    Anti-Tn-antigen monoclonal antibody MLS128 has affinity for three consecutive Tn-antigens (Tn3) more than Tn2. The major aim of this study was to isolate genes encoding MLS128 variable domains to produce a large quantity of recombinant MLS128 antibodies, in turn, allowing the conduct of studies on precise interactions between Tn3- or Tn2-epitopes and MLS128. This study describes cloning of the variable region genes of MLS128, construction of the variable region genes in single-chain variable fragments (scFv) and two scFvs conjugated with human IgG(1) hinge and Fc regions (scFv-Fc) types, and their respective expression in bacterial and mammalian cell. MLS128 scFv protein with the expected specificity and affinity was successfully prepared from inclusion bodies accumulating in Escherichia coli. Construction, expression and purification of two types of MLS128-scFv-Fc proteins with differing linker lengths in Chinese hamster ovary cells demonstrated that the purified scFv-Fc proteins had binding activity specific to the glycoprotein-expressing Tn-antigen clusters. These results revealed that VL and VH genes cloned from the hybridoma represent those of MLS128 and that recombinant antibodies produced from these genes should provide sufficient amounts of binding domains for use in 3D structural studies such as NMR and X-ray analysis. PMID:22318767

  20. Burleson County State of the Community Report 

    E-print Network

    Cousins, Tiffany; English, Jeffrey; Meng, Yi

    2015-01-01

    ://co.burleson.tx.us/ BurlesonCoFactSheet. http://www.burlesoncountytx.com/visit/Burleson%20County%20Resource%20Gui de%202008.pdf BVCOG Burleson Hazard Mitigation Plan http://www.co.burleson.tx.us/image/files/Emergency%20Management/Burleson %20County%20Mitigation%20Plan%20...Burleson County 1 1 Burleson County Seal: Edited from http://www.co.burleson.tx.us/image/images/Burleson%20County%20Seal.JPG 2015 STATE OF THE COMMUNITY REPORT TIFFANY COUSINS, JEFFREY...

  1. Up-promoter mutations in the positively-regulated mer promoter of Tn501.

    PubMed Central

    Lund, P; Brown, N

    1989-01-01

    Transcription from the mer promoter of transposon Tn501 is repressed by MerR (the product of the merR gene) in the absence of Hg2+, and activated by MerR in the presence of Hg2+. In the absence of MerR, the mer promoter has weak constitutive activity. The DNA sequence of the mer promoter shows candidate -35 and -10 sequences at the unusually high spacing of 19 base-pairs. We have selected for spontaneous mutations in the mer promoter that confer an up-promoter phenotype. Four different mutants have been isolated. Three of these are single base-pair deletions between the -10 and -35 sequences. A fourth removes the -10 sequence entirely, and places a second potential -10 sequence 17 base-pairs from the -35 sequence. None of these mutant promoters are induced by MerR in the presence of Hg2+. Two of them are repressed by MerR irrespective of the presence or absence of Hg2+. Models for the mode of action of the MerR protein are discussed in the light of these results. Our data support a mechanism in which the MerR protein in the presence of Hg2+ acts to change the conformation of DNA in the mer promoter. Images PMID:2548164

  2. Understanding the structural specificity of Tn antigen for its receptor: an NMR solution study.

    PubMed

    D'Amelio, Nicola; Coslovi, Anna; Rossi, Marco; Uggeri, Fulvio; Paoletti, Sergio

    2012-04-01

    The present work aims at understanding the structural basis of the biological recognition of Tn antigen (GalNAc-?-O-L-Ser), a specific epitope expressed by tumor cells, and the role of its amino acidic moiety in the interaction with its receptor (the isolectin B4 extracted from Vicia villosa). An NMR structural characterization of the ? and ? anomers, based on J couplings and molecular modeling revealed a structure in very good agreement with data reported in literature for variants of the same molecules. In order to demonstrate the involvement of the amino acid in the ligand-receptor recognition, also GalNAc-?-O-D-Ser was studied; the change in the stereochemistry is in fact expected to impact on the interaction only in case the serine is part of the epitope. Relaxation properties in the presence of the receptor clearly indicated a selective recognition of the natural L form, probably due to the formation of a water-mediated hydrogen bond with Asn 129 of the protein. PMID:22341503

  3. Antiferromagnetic pinning of a phase-like mode below TN in NdMnO3

    NASA Astrophysics Data System (ADS)

    Massa, Nestor; Del Campo, Leire; de Sousa Meneses, Domingos; Echegut, Patrick; Martínez-Lope, Maria Jesus; Alonso, Jose Antonio

    2012-02-01

    We report on reflectivity and emission far infrared spectra of NdMnO3 between4K and its dissociation temperature. Phonon bands at 300K are in agreement with orthorhombic Pbnm space group assignments. In addition, a broad strong band. reminiscent to a phase-mode in quasi-one dimension metals, is found at very low frequencies that it is understood originating in charge fluctuations in d-orbitals. There is no distinctive behaviors between 1073 K and 1173 K, where orthorhombic O and O' coexist. Beyond ˜700 K a mid-infrared polaron band turns into a Drude tail suggesting hopping conductivity double exchange due to air heating oxidation Mn^3+-> Mn^4+ +1e^-. Below 300 K phonons are better defined and the low frequency giant dipole acquires strength. Few degrees above the antiferromagnetic transition it broadens as electrons loosing coherence. At TN˜76 K we find strong phonon magnetostriction while the band turns asymmetric locking-in to the underlying magnetic order. Preliminary measurements of hexagonal TmMnO3, show that asymmetry split due to lower symmetry and the triangular magnetic lattice two exchange integrals J1 and J2 in the a-b plane. Similar to a soft mode those two bands and a lower frequency resonance undergo strong hardening down to 4 K.

  4. The novel tetramethylpyrazine bis-nitrone (TN-2) protects against MPTP/MPP+-induced neurotoxicity via inhibition of mitochondrial-dependent apoptosis.

    PubMed

    Xu, Daping; Duan, Hongwei; Zhang, Zaijun; Cui, Wei; Wang, Liang; Sun, Yewei; Lang, Ming; Hoi, Pui Man; Han, Yifan; Wang, Yuqiang; Lee, Simon MingYuen

    2014-03-01

    Mitochondrial-dependent apoptosis plays an important role in the degeneration of dopaminergic neurons in Parkinson's disease (PD). Methyl-4-phenyl-1,2,3,6-tetra- hydropyridine (MPTP), the most widely used neurotoxin to simulate PD, is converted to 1-methyl-4-phenylpyridinium (MPP(+)) in vivo. MPP(+) induces excessive intracellular reactive oxygen species (ROS), leading to mitochondrial-dependent apoptosis via sequentially opening mitochondria permeability transition pore (mPTP) to release cytochrome c from mitochondria into cytoplasm and activate pro-apoptotic caspase proteins. We have previously synthesized 2,5-[[(1,1-dimethylethyl)oxidoimino]methyl]-3,6-trimethylpyrazine (TN-2), a novel derivative of the Chinese herb medicine tetramethylpyrazine (TMP). TN-2 is armed with two powerful free radical-scavenging nitrone moieties. TN-2 significantly reversed the loss of dopaminergic neurons in the substantia nigra and the decrease in dopamine level in the striatum induced by MPTP in mice. TN-2 ameliorated the MPTP-induced decrease of brain superoxide dismutase activity and glutathione concentration and increase of brain malondialdehyde. In addition, TN-2 inhibited MPP(+)-induced neuronal damage/apoptosis in primary cerebellum granular neurons (CGNs) and SH-SY5Y cells. TN-2 decreased excessive intracellular ROS, prevented the loss of mitochondrial membrane potential, blocked the release of mitochondrial cytochrome c and inhibited the activation of caspase-3 and caspase-9. Moreover, TN-2 treatment increased the mRNA expression of mitochondrial biogenesis factors peroxisome proliferator-activated receptor (PPAR)-? coactivator-1 (PGC- 1? and ?) and mitochondrial transcription factor A (Tfam) in SH-SY5Y cells and CGNs. These results suggest that TN-2 protects dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity via the inhibition of mitochondrial-dependent apoptosis and possibly via the activation of mitochondrial biogenesis, indicating that TN-2 is a potential new treatment for PD. PMID:24233519

  5. Defining essential genes and identifying virulence factors of Porphyromonas gingivalis by massively parallel sequencing of transposon libraries (Tn-seq).

    PubMed

    Klein, Brian A; Duncan, Margaret J; Hu, Linden T

    2015-01-01

    Porphyromonas gingivalis is a keystone pathogen in the development and progression of periodontal disease. Obstacles to the development of saturated transposon libraries have previously limited transposon mutant-based screens as well as essential gene studies. We have developed a system for efficient transposon mutagenesis of P. gingivalis using a modified mariner transposon. Tn-seq is a technique that allows for quantitative assessment of individual mutants within a transposon mutant library by sequencing the transposon-genome junctions and then compiling mutant presence by mapping to a base genome. Using Tn-seq, it is possible to quickly define all the insertional mutants in a library and thus identify nonessential genes under the conditions in which the library was produced. Identification of fitness of individual mutants under specific conditions can be performed by exposing the library to selective pressures. PMID:25636611

  6. Identification of Genes Involved in Swarming Motility Using a Pseudomonas aeruginosa PAO1 Mini-Tn5-lux Mutant Library?

    PubMed Central

    Overhage, Joerg; Lewenza, Shawn; Marr, Alexandra K.; Hancock, Robert E. W.

    2007-01-01

    During a screening of a mini-Tn5-luxCDABE transposon mutant library of Pseudomonas aeruginosa PAO1 for alterations in swarming motility, 36 mutants were identified with Tn5 insertions in genes for the synthesis or function of flagellin and type IV pilus, in genes for the Xcp-related type II secretion system, and in regulatory, metabolic, chemosensory, and hypothetical genes with unknown functions. These mutants were differentially affected in swimming and twitching motility but in most cases had only a minor additional motility defect. Our data provide evidence that swarming is a more complex type of motility, since it is influenced by a large number of different genes in P. aeruginosa. Conversely, many of the swarming-negative mutants also showed an impairment in biofilm formation, indicating a strong relationship between these types of growth states. PMID:17158671

  7. First steps towards the genetic manipulation of Mycoplasma agalactiae and Mycoplasma bovis using the transposon Tn4001mod.

    PubMed

    Chopra-Dewasthaly, Rohini; Zimmermann, Martina; Rosengarten, Renate; Citti, Christine

    2005-01-01

    Mycoplasma agalactiae and M. bovis rank amongst the most serious pathogenic mycoplasmas infecting small ruminants and cattle, respectively. Despite considerable advances made in Mycoplasma molecular genetics in the past decade, there is still a complete lack of genetic tools to assess the pathogenic mechanisms of these two species. Studies were undertaken to develop a genetic system for the analysis of potential virulence factors of these pathogens. Transposon Tn4001mod was successfully introduced into various chromosomal sites of M. agalactiae and M. bovis with an optimal frequency of 10(-6) per viable colony-forming unit (CFU). This is the first report that demonstrates the amenability of these agents to transformation and to genetic manipulation. Furthermore, Tn4001 is implicated as the first potential genetic tool available for these ruminant pathogens. PMID:15715173

  8. Chester County ground-water atlas, Chester County, Pennsylvania

    USGS Publications Warehouse

    Ludlow, Russell A.; Loper, Connie A.

    2004-01-01

    Chester County encompasses 760 square miles in southeastern Pennsylvania. Groundwater- quality studies have been conducted in the county over several decades to address specific hydrologic issues. This report compiles and describes water-quality data collected during studies conducted mostly after 1990 and summarizes the data in a county-wide perspective. In this report, water-quality constituents are described in regard to what they are, why the constituents are important, and where constituent concentrations vary relative to geology or land use. Water-quality constituents are grouped into logical units to aid presentation: water-quality constituents measured in the field (pH, alkalinity, specific conductance, and dissolved oxygen), common ions, metals, radionuclides, bacteria, nutrients, pesticides, and volatile organic compounds.Waterquality constituents measured in the field, common ions (except chloride), metals, and radionuclides are discussed relative to geology. Bacteria, nutrients, pesticides, and volatile organic compounds are discussed relative to land use. If the U.S. Environmental Protection Agency (USEPA) or Chester County Health Department has drinkingwater standards for a constituent, the standards are included. Tables and maps are included to assist Chester County residents in understanding the water-quality constituents and their distribution in the county. Ground water in Chester County generally is of good quality and is mostly acidic except in the carbonate rocks and serpentinite, where it is neutral to strongly basic. Calcium carbonate and magnesium carbonate are major constituents of these rocks. Both compounds have high solubility, and, as such, both are major contributors to elevated pH, alkalinity, specific conductance, and the common ions. Elevated pH and alkalinity in carbonate rocks and serpentinite can indicate a potential for scaling in water heaters and household plumbing. Low pH and low alkalinity in the schist, quartzite, and gneiss rocks can indicate a potential for corrosive water. The only constituent measured in the field that has a USEPA Secondary Maximum Contaminant Level (SMCL) is pH. The SMCL for pH is 6.5-8.5; 64 percent of samples analyzed for pH were acidic (below pH 6.5). Only 1 percent of samples were basic (above pH 8.5). Of the common ions, the USEPA has SMCLs for chloride, sulfate, and total dissolved solids. The USEPA has a SMCL and a Primary Maximum Contaminant Level (PMCL) for fluoride. Chloride is more closely related to land use than geology. In Chester County, chloride exceeded the SMCL (250 mg/L) only in 5 percent of the services (commercial services, community services, and military) land-use areas. No samples analyzed for sulfate exceeded the SMCL (250 mg/L). Only 3 percent of samples analyzed for total dissolved solids exceeded the SMCL (500 milligrams per liter) (mg/L). No samples analyzed for fluoride equaled or exceeded the SMCL (2.0 mg/L) or PMCL (4.0 mg/L). Iron concentrations exceeded the USEPA SMCL in 11 percent of samples and were highest in schist (14 percent) and gneiss (13 percent). Manganese concentrations exceeded the SMCL in 19 percent of samples and were highest in quartzite and schist (both 28 percent). Lead and arsenic were present in low concentrations: the highest concentrations of lead occurred in water from quartzite (8 percent exceeded the USEPA Action Level), and arsenic was detected mostly in Triassic sedimentary rocks (9 percent exceeded the USEPA PMCL). The highest concentrations of copper occurred more frequently in quartzite rocks, and to a lesser extent were evenly distributed between ground water in gneiss, schist, and Triassic sedimentary rocks. Elevated concentrations of radon-222 and the combined radium-226/radium-228 radionuclides were common in water from quartzite and schist. Gross alpha and gross beta particle activities were elevated in water from quartzite and carbonate rocks. In contrast, elevated concentrations of uranium prima

  9. Sialyl-Tn antigen as a marker of colon cancer risk in ulcerative colitis: Relation to dysplasia and DNA aneuploidy

    Microsoft Academic Search

    Per Karlén; Eric Young; Olle Broström; Robert Löfberg; Bernhard Tribukait; Åke Öst; Carol Bodian; Steven Itzkowitz

    1998-01-01

    Background & Aims: Expression of the mucin-associated carbohydrate antigen sialyl-Tn (STn) and DNA aneuploidy has each been shown to correlate with malignant transformation in patients with sporadic colon cancer and in those with ulcerative colitis (UC). This study aimed to determine how STn expression topographically and temporally relates to aneuploidy and neoplasia in patients with long-standing UC. Methods: Twenty-six patients

  10. Molecular and biochemical characterization of a new alkaline ?-propeller phytase from the insect symbiotic bacterium Janthinobacterium sp. TN115

    Microsoft Academic Search

    Rui Zhang; Peilong Yang; Huoqing Huang; Tiezheng Yuan; Pengjun Shi; Kun Meng; Bin Yao

    A phytase-encoding gene (phyA115) was cloned from Janthinobacterium sp. TN115, a symbiotic bacterial strain isolated from the gut contents of Batocera horsfieldi larvae (Coleoptera: Cerambycidae), and expressed in Escherichia coli. The 1,884-bp full-length gene encodes a 28-residue putative signal peptide and a 599-residue mature protein with a calculated\\u000a mass of 64 kDa. The deduced PhyA115 shares low identity with known sequences

  11. First Report of an OXA-23 Carbapenemase-Producing Acinetobacter baumannii Clinical Isolate Related to Tn2006 in Spain

    PubMed Central

    Espinal, P.; Macià, M. D.; Roca, I.; Gato, E.; Ruíz, E.; Fernández-Cuenca, F.; Oliver, A.; Rodríguez-Baño, J.; Bou, G.; Tomás, M.

    2013-01-01

    A carbapenem-resistant Acinetobacter baumannii clinical isolate belonging to European clone II and sequence type 2 was recovered from a patient in the Son Espases hospital in Mallorca, Spain. Genetic analysis showed the presence of the blaOXA-23 gene in association with the widely disseminated transposon Tn2006. This is the first reported identification of A. baumannii carrying blaOXA-23 in Spain. PMID:23070166

  12. Precise Insertion of Antibiotic Resistance Determinants into Tn21Like Transposons: Nucleotide Sequence of the OXA-1 beta -lactamase Gene

    Microsoft Academic Search

    Marc Ouellette; Luc Bissonnette; Paul H. Roy

    1987-01-01

    Several plasmid-encoded beta -lactamase are on multiresistance transposable elements. The OXA-1 beta -lactamase gene is part of Tn2603, which is borne on the R plasmid RGN238. We report here the complete nucleotide sequence of the OXA-1 beta -lactamase gene and flanking sequences. The OXA-1 gene shows a >50% sequence divergence from the OXA-2 gene, yet there is significant functional similarity

  13. A high-performance short-channel bottom-contact OTFT and its application to AM-TN-LCD

    Microsoft Academic Search

    Kazumasa Nomoto; Nobukazu Hirai; Nobuhide Yoneya; Noriyuki Kawashima; Makoto Noda; Masaru Wada; Jiro Kasahara

    2005-01-01

    We have demonstrated an organic thin-film-transistor (OTFT)-driven active-matrix twisted-nematic liquid crystal display (AM-TN-LCD) on a glass substrate, with a resolution of 160×120 pixels, 79 ppi. Substrate temperature was kept below the plastic-compatible temperature of 180°C throughout the fabrication process. In order to realize an OTFT-driven display with fine resolution, we employed short-channel bottom-contact (BC) pentacene OTFTs. It has been known

  14. Isolation of transposon Tn 5 insertion mutants of Rhodobacter capsulatus unable to reduce trimethylamine-N-oxide and dimethylsulphoxide

    Microsoft Academic Search

    D. J. Kelly; D. J. Richardson; S. J. Ferguson; J. B. Jackson

    1988-01-01

    1) Rhodobacter capsulatus (formerly Rhodopseudomonas capsulata) strain 37b4 was subjected to transposon Tn5 mutagenesis. 2) Kanamycin-resistant transconjugants were screened for their inability to reduce trimethylamine-N-oxide (TMAO) as judged by the lack of alkali production during anaerobic growth on plates containing glucose as carbon source and cresol red as pH indicator. 3) Of 6 mutants examined, all were found to have

  15. Analysis for prevalence and physical linkages amongst integrons, ISEcp1, ISCR1, Tn21 and Tn7 encountered in Escherichia coli strains from hospitalized and non-hospitalized patients in Kenya during a 19-year period (1992–2011)

    PubMed Central

    2013-01-01

    Background We determined the prevalence and evidence for physical linkage amongst integrons, insertion sequences, Tn21 and Tn7 transposons in a collection of 1327 E. coli obtained over a 19-year period from patients in Kenya. Results The prevalence of class 1 integrons was 35%, class 2 integrons were detected in 3 isolates but no isolate contained a class 3 integron. Integron lacking the 3’-CS or those linked to sul3 gene or IS26 or those containing the ISCR1 were only detected in multidrug resistant (MDR) strains. The dfrAs were the most common cassettes and their prevalence was: - dfrA1(28%), dfrA12(20%), dfA17(9%), dfrA7(9%), and dfrA16(5%). The aadA were the second most abundant cassettes and their prevalence was: - aadA1(25%), aadA2(21%), and aadA5(14%). Other cassettes occurred in lower prevalence of below 5%. Prevalence of Tn21, ISEcp1, ISCR1 and IS26 was 22%, 10%, 15%, and 7% respectively. Majority of Tn21 containing integrons carried a complete set of transposition genes while class 2 integrons were borne on Tn7 transposon. The qnrA genes were detected in 34(3%) isolates while 19(1%) carried qnrB. All qnr genes were in MDR strains carrying integrons containing the ISCR1. Close to 88% of blaTEM-52 were linked to IS26 while???80% of blaCTX-Ms and blaCMYs were linked to ISEcp1. Only a few studies have identified a blaCTX-M-9 containing an ISEcp1 element as reported in this study. Multiple genetic elements, especially those borne on incIl, incFII, and incL/M plasmids, and their associated resistance genes were transferrable en bloc to E. coli strain J53 in mating experiments. Conclusions This is the first detailed study on the prevalence of selected elements implicated in evolution of resistance determinants in a large collection of clinical E. coli in Africa. Proliferation of such strains carrying multiple resistance elements is likely to compromise the use of affordable and available treatment options for majority of poor patients in Africa. There is therefore a need to monitor the spread of these highly resistant strains in developing countries through proper infection control and appropriate use of antimicrobials. PMID:23682924

  16. Characterization of transposon Tn5-facilitated donor strains and development of a chromosomal linkage map for Agrobacterium tumefaciens.

    PubMed Central

    Pischl, D L; Farrand, S K

    1984-01-01

    We have further characterized the transposon Tn5-facilitated chromosomal gene transfer system developed for Agrobacterium tumefaciens 15955. Using a strain whose chromosome contained Tn5, we compared the chromosome-mobilizing ability of plasmid pDP37 (containing Tn5) with that of its parent plasmid R68.45. For R68.45, we observed nonpolar transmission from multiple origins. For pDP37 we found polarized transmission from a single origin near ilv. When we examined the transmission gradients of a number of pDP37-containing donor strains each differing at the site of the chromosomal insertion we found just two classes. One set of donors transmitted markers with a gradient of Ilv+ greater than Rifr greater than His+ greater than Met+, whereas the second set transferred His+ greater than Rifr greater than Ilv+ greater than Met+. Using representatives from each transmission class of donor strains, we conducted matings to measure the degree of linkage between pairs of adjacent donor markers. From this information we developed a map of the A. tumefaciens 15955 chromosome. Attempts to isolate R-prime plasmids or Hfr-like donors were unsuccessful. PMID:6330023

  17. Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant.

    PubMed

    Rouws, Luc F M; Simões-Araújo, Jean L; Hemerly, Adriana S; Baldani, José I

    2008-04-01

    Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5Tnp Transposome (Epicentre). Up to 1 x 10(6) mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. PMID:18060666

  18. Spatial variability assessment of soil nutrients in an intense agricultural area, a case study of Rugao County in Yangtze River Delta Region, China

    NASA Astrophysics Data System (ADS)

    Zhao, Yongcun; Xu, Xianghua; Darilek, Jeremy Landon; Huang, Biao; Sun, Weixia; Shi, Xuezheng

    2009-05-01

    Topsoil samples (0-20 cm) ( n = 237) were collected from Rugao County, China. Geostatistical variogram analysis, sequential Gaussian simulation (SGS), and principal component (PC) analysis were applied to assess spatial variability of soil nutrients, identify the possible areas of nutrient deficiency, and explore spatial scale of variability of soil nutrients in the county. High variability of soil nutrient such as soil organic matter (SOM), total nitrogen (TN), available P, K, Fe, Mn, Cu, Zn, and B concentrations were observed. Soil nutrient properties displayed significant differences in their spatial structures, with available Cu having strong spatial dependence, SOM and available P having weak spatial dependence, and other nutrient properties having moderate spatial dependence. The soil nutrient deficiency, defined here as measured nutrient concentrations which do not meet the advisory threshold values specific to the county for dominant crops, namely rice, wheat, and rape seeds, was observed in available K and Zn, and the deficient areas covered 38 and 11%, respectively. The first three PCs of the nine soil nutrient properties explained 62.40% of the total variance. TN and SOM with higher loadings on PC1 are closely related to soil texture derived from different parent materials. The PC2 combined intermediate response variables such as available Zn and P that are likely to be controlled by land use and soil pH. Available B has the highest loading on PC3 and its variability of concentrations may be primarily ascribed to localized anthropogenic influence. The amelioration of soil physical properties (i.e. soil texture) and soil pH may improve the availability of soil nutrients and the sustainability of the agricultural system of Rugao County.

  19. Mn-oxidizing Bacteria in Oak Ridge, TN and the Potential for Mercury Remediation

    NASA Astrophysics Data System (ADS)

    Wright, K. L.; McNeal, K. S.; Han, F. X.

    2012-12-01

    East Fork Poplar Creek (EFPC) in Oak Ridge, TN was highly contaminated with elemental mercury in the 1950 and 1960. The area is still experiencing the effects of mercury contamination, and researchers are searching for ways to remediate the EFPC. One possible mechanism for bioremediation is the use of biogenic Mn oxides to remove heavy metals from water systems. Six native Pseudomonas bacteria species were isolated from the EFPC in order to examine biogenic Mn oxides production and bioremediation of Oak Ridge slurries. To investigate the biochemical interactions of Pseudomonas and the native microbial communities with Hg, Mn, Fe, S, six different slurry treatment groups were compared using inductively coupled plasma-atomic emission spectrometry (ICP-AES) and cold vapor atomic absorption spectrometry (CVAAS). Oak Ridge slurries were autoclaved to inhibit microbial growth (group 1), autoclaved and amended with HgS (group 2), autoclaved and amended with Pseudomonas isolates and additional HgS (group 3), untreated slurry (group 4), normal slurry amended with HgS (group 5), and normal slurry amended with Pseudomonas isolates and additional HgS (group 6). The comparison of the autoclaved groups with the counterpart untreated and normal Oak Ridge slurries highlighted important microbial interactions. Also, the Pseudomonas isolates were grown separately in a MnSO4 media, and the individual bacteria were monitored for Mn-oxidization using ICP-AES and transmission electron microscopy (TEM). In the slurry sediments, the Pseudomonas isolates did produce Mn oxides which bound to mercury, and mercury bound to organic matter significantly decreased. However, after a significant decrease of dissolved mercury in the water, dissolved mercury was cycled back into the water system on day 10 of the study. Additionally, two individual native Oak Ridge Pseudomonas isolates demonstrated Mn-oxidization. Biogenic Mn oxides have the potential to decrease mercury cycling, however there is need for more in depth and long-term studies to confirm their sustained use as Hg bioremediators.

  20. Monroe County Library System: Pathfinders

    NSDL National Science Digital Library

    Named after President James Monroe, Monroe County is part of upstate New York. The industrial metropolis of Rochester sits within its boundaries, and one of its key institutions is the Monroe County Library System. Over the past several years, the System has created this treasure trove of digital collections which dissect and organize the history of the area through historic maps, architectural guides, and an online history tour of places in Rochester that played a role in the abolition movement and the Civil War. First-time visitors might want to start out by looking over the "Raising the Flag: Patriotism in Rochester, 1892-1922" photo gallery and then move on the Susan B. Anthony Letters Collection. Library staff members have also created nine fine research guides for scholars and others, and they include a guide to Rochester immigrants and a house history guide.

  1. Lake County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    .6% Diabetes prevalence 6.7% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.5% 4.1% 6.0% All 152.7 115.4 Myocardial Infarction (Heart Attack) Per 100,000 population 1 161.2 147.3 1 Community County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. Unintentional Injuries** 1. Cancer 2. Heart

  2. Daniels County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community Health Data, MT Dept.2 Diabetes Per 100,000 population 1 156.4 (Region 1) 115.4 Myocardial Infarction (Heart Attack) Per 100 Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease

  3. Chouteau County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 4.4% 4.1% 6.0% All Sites Cancer 461.9 455.5 543.2 1 Community Health Data, MT Dept 180.2 182.2 Diabetes Per 100,000 population 1 86.4 115.4 Myocardial Infarction (Heart Attack) Per 100 Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease

  4. Cascade County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    prevalence (Heart Attack) 4.0% 4.1% 6.0% All Sites Cancer 461.9 (Region 2) 455.5 543.2 1 Community Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12

  5. Ravalli County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease.0% Breast Cancer (Mammogram in past 2 yrs) 1 82.1% 71.9% Blood Stool 1 25.0% 25.3% Sigmoidoscopy Attack) 4.9% 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455.5 543.2 1 Community Health Data, MT Dept

  6. Organisation of the bph gene cluster of transposon Tn 4371, encoding enzymes for the degradation of biphenyl and 4-chlorobiphenyl compounds

    Microsoft Academic Search

    J. W. Lengeler C. Merlin; D. Springael; M. Mergeay; A. Toussaint

    1997-01-01

    Tn4371 is a 55 kb transposon which encodes enzymes for the degradation of biphenyl and 4-chlorobiphenyl compounds into benzoate\\u000a and 4-chlorobenzo-ate derivatives. We constructed a cosmid library of Tn4371 DNA. The bph genes involved in biphenyl\\/4-chlorobiphenyl degradation were found to be clustered in the middle of the transposon. Sequencing\\u000a revealed an organisation of the bph genes similar to that previously

  7. Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen

    Microsoft Academic Search

    Maria Duk; Albert M. Wu; Elwira Lisowska

    1994-01-01

    Earlier studies showed thatMoluccella laevis lectin, which has anti-Tn specificity, reacts more strongly with native or desialylated blood group N glycophorin A than with the respective glycophorins of blood group M. We now present results indicating thatVicia villosa B4 anti-Tn lectin, which does not show detectable reaction with untreated glycophorins or erythrocytes, reacts better with desialylated blood group N antigen

  8. 71 FR 20072 - Clarke County Water Supply Project, Clarke County, IA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2006-04-19

    ...DEPARTMENT OF AGRICULTURE Natural Resources Conservation...Service Clarke County Water Supply Project, Clarke...U.S. Department of Agriculture gives notice that an...for the Clarke County Water Supply Project,...

  9. Genome-wide investigation of the genes involved in nicotine metabolism in Pseudomonas putida J5 by Tn5 transposon mutagenesis.

    PubMed

    Xia, Zhenyuan; Zhang, Wei; Lei, Liping; Liu, Xingzhong; Wei, Hai-Lei

    2015-08-01

    Pseudomonas putida J5 is an efficient nicotine-degrading bacterial strain isolated from the tobacco rhizosphere. We successfully performed a comprehensive whole-genome analysis of nicotine metabolism-associated genes by Tn5 transposon mutagenesis in P. putida J5. A total of 18 mutants with unique insertions screened from 16,324 Tn5-transformants failed to use nicotine as the sole carbon source. Flanking sequences of the Tn5 transposon were cloned with a shotgun method from all of the nicotine-growth-deficient mutants. The potentially essential products of mutated gene were classified as follows: oxidoreductases, protein and metal transport systems, proteases and peptidases, transcriptional and translational regulators, and unknown proteins. Bioinformatic analysis of the Tn5 insertion sites indicated that the nicotine metabolic genes were separated and widely distributed in the genome. One of the mutants, M2022, was a Tn5 insert into a gene encoding a homolog of 6-hydroxy-L-nicotine oxidase, the second enzyme of nicotine metabolism in Arthrobacter nicotinovorans. Genetic and biochemical analysis confirmed that three open reading frames (ORFs) from an approximately 13-kb fragment recovered from the mutant M2022 were responsible for the transformation of nicotine to 3-succinoyl-pyridine via pseudooxynicotine and 3-succinoyl semialdehyde-pyridine, the first three steps of nicotine degradation. Further research on these mutants and the Tn5-inserted genes will help us characterize nicotine metabolic processes in P. putida J5. PMID:25808517

  10. Involvement of a chlorobenzoate-catabolic transposon, Tn5271, in community adaptation to chlorobiphenyl, chloroaniline, and 2,4-dichlorophenoxyacetic acid in a freshwater ecosystem

    SciTech Connect

    Fulthorpe, R.R.; Wyndham, R.C. (Carleton Univ., Ottawa, Ontario (Canada))

    1992-01-01

    A chlorobenzoate-catabolic transposon (Tn5271) was introduced on a conjugative plasmid (pBRC60) in the natural host, Alcaligenes sp. strain BR60, into lake water and sediment flowthrough microcosms. Experimental microcosms were exposed to micromolar levels of 3-chlorobenzoate, 4-chloroaniline, 2,4-dichlorophenoxyacetate, or 3-chlorobiphenyl. The populations of the host, BR60, and organisms carrying Tn5271 were monitored over a 100-day period by use of selective plate counts and the most-probable-number-DNA hybridization method. Populations of Tn5271-carrying bacteria were significantly higher in microcosms dosed with 3-chlorobenzoate, 4-chloroaniline, and 3-chlorobiphenyl than in the control microcosms, indicating that each of these chemicals exerts a selective force on this particular genotype in natural systems. The rates of 3-chlorobenzoate uptake and respiration correlated with Tn5271-carrying populations, as did the rates of 4-chloroaniline uptake and respiration. Plasmid transfer in the 3-chlorobenzoate- and 3-chlorobiphenyl-dosed microcosms resulted in the selection of three phenotypic clusters of chlorobenzoate degraders, only one of which was closely related to the original pBRC60 (Tn5271) donor, Alcaligenes sp. strain BR60. Bacteria dominating 3-chloroaniline-dosed microcosms carried IS1071, the class II insertion sequence that brackets Tn5271, on a plasmid unrelated to pBRC60. The importance of plasmid transfer and transposition during chemical adaptation is discussed.

  11. Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition

    SciTech Connect

    Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Matsuura, Masaaki [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University (Japan); Gomi, Yasuyuki [Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University (Japan); Takahashi, Michiaki [Research Foundation for Microbial Diseases of Osaka University (Japan); Yamanishi, Koichi [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.j [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Division of Clinical Virology, Kobe University Graduate School of Medicine (Japan)

    2010-06-20

    Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZ{alpha}-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cells as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.

  12. Involvement of a chlorobenzoate-catabolic transposon, Tn5271, in community adaptation to chlorobiphenyl, chloroaniline, and 2,4-dichlorophenoxyacetic acid in a freshwater ecosystem.

    PubMed Central

    Fulthorpe, R R; Wyndham, R C

    1992-01-01

    A chlorobenzoate-catabolic transposon (Tn5271) was introduced on a conjugative plasmid (pBRC60) in the natural host, Alcaligenes sp. strain BR60, into lake water and sediment flowthrough microcosms. Experimental microcosms were exposed to micromolar levels of 3-chlorobenzoate, 4-chloroaniline, 2,4-dichlorophenoxyacetate, or 3-chlorobiphenyl. The populations of the host, BR60, and organisms carrying Tn5271 were monitored over a 100-day period by use of selective plate counts and the most-probable-number-DNA hybridization method. Populations of Tn5271-carrying bacteria were significantly higher in microcosms dosed with 3-chlorobenzoate, 4-chloroaniline, and 3-chlorobiphenyl than in the control microcosms, indicating that each of these chemicals exerts a selective force on this particular genotype in natural systems. The rates of 3-chlorobenzoate uptake and respiration correlated with Tn5271-carrying populations, as did the rates of 4-chloroaniline uptake and respiration. Plasmid transfer in the 3-chlorobenzoate- and 3-chlorobiphenyl-dosed microcosms resulted in the selection of three phenotypic clusters of chlorobenzoate degraders, only one of which was closely related to the original pBRC60 (Tn5271) donor, Alcaligenes sp. strain BR60. Bacteria dominating 4-chloroaniline-dosed microcosms carried IS1071, the class II insertion sequence that brackets Tn5271, on a plasmid unrelated to pBRC60. The importance of plasmid transfer and transposition during chemical adaptation is discussed. Images PMID:1311543

  13. Jefferson County State of the Community Report 

    E-print Network

    Chen, Xi; Lee, Kanghyun; Aldalbahi, Faisal

    2015-01-01

    courtesy to their neighbors and for the safety of pedestrians. Residents of incorporated areas are urged to check with their city governments for any policies regarding snow re- moval from sidewalks. Residents in unincorporated areas of Jefferson County... Thoroughfare Plan STATE OF COMMUNITY REPORT I 19 JEFFERSON COUNTY, COLORADO Source: Jefferson County Countywide Transportation Plan, 2014 Bicycle and Pedestrian Plan Bicycling and walking are increasingly important means of transportation for Jefferson...

  14. Liberty County State of the Community Report

    E-print Network

    Shakra, Vera Abou; Page, Hilary; Yan, Wenqian

    2015-01-01

    Liberty County PLAN 613, Spring 2015 LAUP Department Texas A&M University Vera Abou Shakra Hilary Page Wenqian Yan 1 Liberty County State of Community Executive Summary This State of Community Report was prepared... as the initial step in the plan-making process. It gathers data from a wide variety of sources in order to gain a more complete picture of current conditions in Liberty County. Due to resource limitations, there was no public participation, though it can...

  15. Welcome to Union County Extension. The service offered to the citizens of Union County is a part-

    E-print Network

    Jawitz, James W.

    Welcome to Union County Extension. The service offered to the citizens of Union County is a part- nership between Union County Board of County Commissioners and the University of Florida/IFAS. The mission provide by visiting us at our website: http://union.ifas.ufl.edu. Basil Bactawar, Director Union County

  16. ~~ COUNTY 208 WATER QUALITY MANAGEMENT PLAN CLARK COUNTY, NEVAD~

    E-print Network

    #12;· ~~ COUNTY 208 WATER QUALITY MANAGEMENT PLAN ",-- CLARK COUNTY, NEVAD~ 208 PLAN May 9, 1978 an Areawide Waste Tr~atment Management Planning Grant from the U.S. Environmental Protection Agency. SCHOOII OF LAW LIBRARY SOUTHERN ILLINOIS UNIVERSITY #12;CLARK COUNTY 208 WATER QUALITY MANAGEMENT PROGRAM

  17. Digital computer processing of LANDSAT data for North Alabama. [Linestone County, Madison County, Jackson County, Marshall County, and DeKalb County

    NASA Technical Reports Server (NTRS)

    Bond, A. D.; Atkinson, R. J.; Lybanon, M.; Ramapriyan, H. K.

    1977-01-01

    Computer processing procedures and programs applied to Multispectral Scanner data from LANDSAT are described. The output product produced is a level 1 land use map in conformance with a Universal Transverse Mercator projection. The region studied was a five-county area in north Alabama.

  18. Groundwater Transport of Organic Compounds in Old Salvage Yard, Oak Ridge, TN - 12089

    SciTech Connect

    Malek-Mohammadi, Siamak [Civil Engineering and Construction Department, Bradley University, Peoria, IL 61625 (United States); Tachiev, Georgio; Roelant, David [Applied Research Center, Florida International University, Miami, FL 33174 (United States); Bostick, Kent; Daniel, Anamary [Pro2Serve Professional Project Services, Inc., Oak Ridge, TN 37830 (United States)

    2012-07-01

    In 1950's and early 1960's during production of nuclear weapons at the US Department of Energy Y-12 National Security Complex in Oak Ridge TN, volatile organic compounds (VOCs) as well as heavy metals, nitrates, and radionuclides were released to the environment. Field investigations revealed that much of this contamination is still present in soil, bedrock, and groundwater. Operational buildings and old disposal facilities at the site have been identified as major sources of contamination. The Old Salvage Yard (OSY) on the western side of the site has long been characterized as the major source of VOC contamination in soil and groundwater. In order to analyze the fate and transport of VOC contamination- including tetrachloroethene (PCE), 1,2- dichloroethene (1,2-DCE), cis-1,2-dichloroethene (cis-1,2-DCE) and vinyl chloride (VC) - in groundwater and soil at the vicinity of OSY, an integrated surface and subsurface flow and transport model has been developed for the Y-12 NSC using the hydrodynamic and transport numerical package, MIKE-SHE. Hydrogeological characteristics of the site such as hydraulic conductivity, and transport parameters such as partitioning coefficients were varied in an effort to delineate subsurface flow and transport pathways, potential downstream impacts on Upper East Fork Poplar Creek, and the potential risk to industrial workers involved in related Decontamination and Decommissioning (D and D) activities. The simulation results were compared with the analytical modeling results previously performed by McLane Environmental Inc. using SESOIL-AT123D. Specific simulations have been performed to investigate the effect of possible remedial action (removing the contaminated surface soil layers) on the fate and transport of VOCs. The results of the MIKE-SHE reported here can be considered as an upper limit for the predicted concentrations. Based on MIKE-SHE results, PCE, 1,2 DCE, cis-1,2-DCE, and VC are sources in soil with potential to equal or exceed industrial groundwater hazard and risk levels at the vicinity of OSY. VOC contaminants in soil and groundwater will decay below industrial groundwater risk and hazard levels within approximately 20 years. Excavation of surface soil layers at the site will considerably reduce the concentration of VOCs in groundwater and the possibility of migration of VOCs to surface waters. (authors)

  19. First results from TN273 studies of the SE Mariana Forearc rift

    NASA Astrophysics Data System (ADS)

    Ribeiro, J. M.; Stern, R. J.; Kelley, K. A.; Shaw, A. M.; Shimizu, N.; Martinez, F.; Ishii, T.; Ishizuka, O.; Manton, W. I.

    2012-12-01

    TN 273 aboard R/V Thomas Thompson (Dec. 22 2011- Jan. 22 2012) studied an unusual region of rifting affecting the southern Mariana forearc S.W. of Guam. The S.E. Mariana Forearc Rift (SEMFR) formed by diffuse tectonic and volcanic deformation (Martinez and Sleeper, this meeting) ~2.7-3.7 Ma ago to accommodate opening of the southernmost Mariana Trough backarc basin. A total of 730 km linear-track of SEMFR seafloor was surveyed with deep-towed side-scan sonar IMI-30. 14 dredges provided samples of SEMFR igneous rocks, analyzed for whole rock (WR) and glass compositions. These new results coupled with results of earlier investigations confirm that SEMFR is dominated by Miocene lavas along with minor gabbro and diabase. SEMFR lavas range in major element composition from primitive basalt to fractionated andesite (Mg# = 0.36-0.73; SiO2 = 50-57 wt%), mainly controlled by crystal fractionation. Rare Earth Element (REE) patterns range from LREE-depleted, N-MORB-like to flat patterns, reflecting different mantle processes (i.e. different sources, degree of melting …). Glassy rinds and olivine-hosted melt inclusions in these lavas contain variable volatile compositions (F = 75-358 ppm, S = 35-1126 ppm, Cl= 74-1400 ppm, CO2 = 15-520 ppm, 0.36-2.36 wt% H2O). SEMFR lavas show spider diagrams with positive anomalies in LILE and negative anomalies in HSFE. SEMFR lavas have backarc basin-like (BAB-like) chemical composition (H2O < 2.5wt%, Ba/Yb~20, Nb/Yb~1 and ?Nd~9) along with stronger enrichment in Rb and Cs than arc and BAB lavas, as demonstrated by their higher Rb/Th and Cs/Ba ratios in WR and glasses, which may reflect the role of the ultra-shallow fluids. Ultra-shallow fluids are derived from the top of the subducting slab, beneath the forearc, where most of the water and the fluid-mobile elements (Rb, Cs, Ba,) are thought to be released (Schmidt and Poli, 1998, EPSL, Savov et al., 2005, G-3). Our results suggest that i) SEMFR lavas formed by metasomatism of a BAB mantle source by ultra-shallow fluids, likely released from subducted sediments and the altered oceanic crust; and ii) the ultra-shallow fluid is aqueous and is characterized by enrichment in Cs and Rb, suggesting that Cs and Rb are decoupled from Ba in ultra-shallow subduction processes.

  20. 75 FR 54419 - Environmental Impact Statement: Yellowstone County, MT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-07

    ...Administration Environmental Impact Statement: Yellowstone County, MT AGENCY: Federal Highway...prepared for a proposed highway project in Yellowstone County, Montana. FOR FURTHER INFORMATION...312 in or near the city of Billings, Yellowstone County, Montana. FHWA previously...

  1. 76 FR 66033 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  2. 76 FR 12316 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  3. 76 FR 29722 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-23

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  4. 76 FR 6116 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  5. 75 FR 50987 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  6. 75 FR 79334 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-20

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. The Madera...

  7. 76 FR 19970 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...responsibilities for the remainder of the year. The Madera County Resource Advisory Committee...

  8. 75 FR 31759 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...Send written comments to Julie Roberts, Madera County Resource Advisory Committee...

  9. 76 FR 61379 - Final Recovery Plan, Bexar County Karst Invertebrates

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-04

    ...Recovery Plan, Bexar County Karst Invertebrates AGENCY: Fish and Wildlife Service...plan, for the nine Bexar County Karst Invertebrates under the Endangered Species Act of...The following nine Bexar County karst invertebrates were listed as endangered species...

  10. 7 CFR 7.25 - County executive director duties.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...or property coming into the custody of the county committee; (3) Serve as counselor to the local administrative area and county convention chairperson on election procedures; and (4) Supervise, under the direction of the county...

  11. 18. Historic American Buildings Survey, 'MAP OF CHESTER COUNTY, PA. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. Historic American Buildings Survey, 'MAP OF CHESTER COUNTY, PA. CORRECTED EMBELISHED AND PUBLISHED BY R. L. BARNES, PHILA. 1856.' - Chester County Prison, 235 West Market Street, West Chester, Chester County, PA

  12. SEXUALLY TRANSMITTED DISEASES AMONG AFRICAN AMERICANS IN ALLEGHENY COUNTY

    E-print Network

    Sibille, Etienne

    SEXUALLY TRANSMITTED DISEASES AMONG AFRICAN AMERICANS IN ALLEGHENY COUNTY A BLACK PAPER County (hereafter referred to as "the County"). Although most sexually transmitted diseases characterized sexually transmitted diseases as "hidden epidemics." STDs are considered hidden because only

  13. 6. Photocopy of illustration on Map of Chester County Pennsylvania, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Photocopy of illustration on Map of Chester County Pennsylvania, by T.J. Kennedy, published by R.L. Barnes, Philadelphia, Pennsylvania, 1856 SOUTHWEST CORNER, 1856 - Bank of Chester County, 17 North High Street, West Chester, Chester County, PA

  14. Lewis & Clark County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Myocardial Infarction prevalence (Heart Attack) 3.3% 4.1% 6.0% All Sites Cancer 416.6 (Region 4) 455 of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Lewis & Clark County Secondary Data Analysis July

  15. Golden Valley County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Infarction prevalence (Heart Attack) 4.3% 4.1% 6.0% All Sites Cancer 510.8 455.5 543.2 1 Community County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. Unintentional Injuries** 1. Cancer 2. Heart Disease 3.CLRD* 1. Heart Disease 2. Cancer 3. CLRD* #12; Golden Valley County Secondary Data

  16. Educational and Demographic Profile: Madera County

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Madera County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

  17. Ex-Offender Populations in Milwaukee County

    E-print Network

    Saldin, Dilano

    up 400- 500 male admits each year. Around 400 African American women from Milwaukee County have been DOC facilities (Jan. 1993-June 2008). The prison population released into Milwaukee County is 88% male and predominately African American. Other 2% White 23% Hispanic 8% African American 67% The education level

  18. Educational and Demographic Profile: Mariposa County.

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Mariposa County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

  19. Socioeconomic Heterogeneity of Mining-Dependent Counties.

    ERIC Educational Resources Information Center

    Nord, Mark; Luloff, A. E.

    1993-01-01

    Although the socioeconomic well-being of all U.S. mining-dependent counties was slightly above the national average in 1990, disaggregation reveals substantial effects of region and mining subsector. In particular, southern and Great Lakes coal-mining counties had significantly lower high school graduation rates and higher poverty and unemployment…

  20. Educational and Demographic Profile: Santa Cruz County

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Santa Cruz County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation provides a framework for enhanced communication and…

  1. Educational and Demographic Profile: Lake County.

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Lake County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced communication…

  2. Geothermal development issues: Recommendations to Deschutes County

    SciTech Connect

    Gebhard, C.

    1982-07-01

    This report discusses processes and issues related to geothermal development. It is intended to inform planners and interested individuals in Deschutes County about geothermal energy, and advise County officials as to steps that can be taken in anticipation of resource development. (ACR)

  3. Groundwater management and protection Madison County, Alabama

    SciTech Connect

    French, J.H.; Strunk, J.W.

    1990-07-01

    Groundwater is extremely important to Madison County as it provides nearly three quarters of the county's drinking water. In recent years, Madison County has increasingly recognized the need to protect its groundwater resource. A supply of usable groundwater is one element of a high quality environment, which can help spur economic development and provide for the needs of a growing population. Without planning protection and understanding of possible consequences, however, economic development and population pressures can cause a gradual degradation of groundwater. In April 1987, the Tennessee Valley Authority (TVA) convened a local groundwater steering group in Madison County. At the first meeting the ground agreed upon these goals: (1) to seek incorporate groundwater protection into the planning and development process for Madison County, (2) to support efforts by Madison County to obtain authority to adopt zoning ordinances and subdivision regulations, and (3) to develop a groundwater management plan for the county. This report provides essential information needed in developing a plan and is based on the following assumptions: the citizens of Madison County value the environment in which they live and wish to protect it from pollution; continued economic development is necessary for a healthy local economy; and a healthy economy can be sustained and nurtured, without degradation of the groundwater resource, through countywide planning, education, and participation.

  4. Presidio County State of the Community Reprort

    E-print Network

    Xiao, Dong; Hwang, Jinuk; McCharen, Rob

    2015-01-01

    Presidio County, Texas is nestled in far west Texas (Trans-Pecos) next to Big Bend National Park. This county presented a unique challenge due to the limited amount of information available to the general public. Request for information were sent...

  5. Crawford County Adult Education Center Program Evaluation

    ERIC Educational Resources Information Center

    Faubus, Deborah L.

    2011-01-01

    The Crawford County Adult Education Program Evaluation was implemented to gather data that would assist in increasing the number of enrolled students at CCAEC. The data were compiled from state, federal, local extant data, a Community Opinion Survey and focus group interviews. Of the 1,015 Crawford County Residents who were emailed the COS, 213…

  6. Santa Clara County Asian Health Survey: Methodology.

    ERIC Educational Resources Information Center

    Tom, Soleng; And Others

    This paper describes the methodology used in a study assessing the health needs of the Asian population, specifically the Chinese, Cambodian, and Vietnamese populations, in Santa Clara County, California. The study by the Santa Clara County Health Department is part of a wide-ranging needs assessment project primarily intended to guide…

  7. A Profile of Anson County, North Carolina.

    ERIC Educational Resources Information Center

    Farr, M. Gaston; And Others

    Since 1950 Anson County, North Carolina, has had major contributions to economic development, a source of great concern to residents of the almost entirely rural area. The increased capacity of the Blewitt Falls Dam power output and the county-wide water filtration system (one of only a few in the United States today) are attractive to industry.…

  8. Fall 2010 Davis County Tuesday Workshop

    NSDL National Science Digital Library

    Brooke Robertshaw

    2010-09-22

    Participant materials for those participating in the Tuesday Fall, 2010 Davis County IA Workshop . For viewing and printing convenience each resource is a pdf. For your convenience, there is a link to download the free Adobe Reader. Download Adobe Reader Workshop Materials Handouts Day 1 Workshop Handout and Materials Davis County Tuesday Day 2 Handouts Davis County Tuesday Day 3 Handouts Reflection Paper Prompts Fall 2010 Davis County Workshops First Reflection Prompts Fall 2010 Davis County Workshops Second Reflection Prompts Sample Instructional Architect Projects Solving One Step equations: Pre-Algebra Standard 3 Objective 2: Students will solve one step equations. The students will use addition and subtraction to solve the equations. Cells: Students will have the opportunity to explore the cell in a 3D fashion and learn more ...

  9. Imperial County geothermal development annual meeting: summary

    SciTech Connect

    Not Available

    1983-01-01

    All phases of current geothermal development in Imperial County are discussed and future plans for development are reviewed. Topics covered include: Heber status update, Heber binary project, direct geothermal use for high-fructose corn sweetener production, update on county planning activities, Brawley and Salton Sea facility status, status of Imperial County projects, status of South Brawley Prospect 1983, Niland geothermal energy program, recent and pending changes in federal procedures/organizations, plant indicators of geothermal fluid on East Mesa, state lands activities in Imperial County, environmental interests in Imperial County, offshore exploration, strategic metals in geothermal fluids rebuilding of East Mesa Power Plant, direct use geothermal potential for Calipatria industrial Park, the Audubon Society case, status report of the Cerro Prieto geothermal field, East Brawley Prospect, and precision gravity survey at Heber and Cerro Prieto geothermal fields. (MHR)

  10. Radioprotection of the brain white matter by Mn(III) n-Butoxyethylpyridylporphyrin-based superoxide dismutase mimic MnTnBuOE-2-PyP5+.

    PubMed

    Weitzel, Douglas H; Tovmasyan, Artak; Ashcraft, Kathleen A; Rajic, Zrinka; Weitner, Tin; Liu, Chunlei; Li, Wei; Buckley, Anne F; Prasad, Mark R; Young, Kenneth H; Rodriguiz, Ramona M; Wetsel, William C; Peters, Katherine B; Spasojevic, Ivan; Herndon, James E; Batinic-Haberle, Ines; Dewhirst, Mark W

    2015-01-01

    Cranial irradiation is a standard therapy for primary and metastatic brain tumors. A major drawback of radiotherapy (RT), however, is long-term cognitive loss that affects quality of life. Radiation-induced oxidative stress in normal brain tissue is thought to contribute to cognitive decline. We evaluated the effectiveness of a novel mimic of superoxide dismutase enzyme (SOD), MnTnBuOE-2-PyP(5+)(Mn(III) meso-tetrakis(N-n-butoxyethylpyridinium-2-yl)porphyrin), to provide long-term neuroprotection following 8 Gy of whole brain irradiation. Long-term RT damage can only be assessed by brain imaging and neurocognitive studies. C57BL/6J mice were treated with MnTnBuOE-2-PyP(5+) before and after RT and evaluated three months later. At this time point, drug concentration in the brain was 25 nmol/L. Mice treated with MnTnBuOE-2-PyP(5+)/RT exhibited MRI evidence for myelin preservation in the corpus callosum compared with saline/RT treatment. Corpus callosum histology demonstrated a significant loss of axons in the saline/RT group that was rescued in the MnTnBuOE-2-PyP(5+)/RT group. In addition, the saline/RT groups exhibited deficits in motor proficiency as assessed by the rotorod test and running wheel tests. These deficits were ameliorated in groups treated with MnTnBuOE-2-PyP(5+)/RT. Our data demonstrate that MnTnBuOE-2-PyP(5+) is neuroprotective for oxidative stress damage caused by radiation exposure. In addition, glioblastoma cells were not protected by MnTnBuOE-2-PyP(5+) combination with radiation in vitro. Likewise, the combination of MnTnBuOE-2-PyP(5+) with radiation inhibited tumor growth more than RT alone in flank tumors. In summary, MnTnBuOE-2-PyP(5+) has dual activity as a neuroprotector and a tumor radiosensitizer. Thus, it is an attractive candidate for adjuvant therapy with RT in future studies with patients with brain cancer. PMID:25319393

  11. Analysis of the Shallow Groundwater Flow System at Fire Island National Seashore, Suffolk County, New York

    USGS Publications Warehouse

    Schubert, Christopher E.

    2010-01-01

    Fire Island National Seashore (FIIS) occupies 42 kilometers of the barrier island for which it is named that lies off the southern shore of Suffolk County, N.Y. Freshwater in the highly permeable, sandy aquifer underlying Fire Island is bounded laterally by marine surface waters and at depth by saline groundwater. Interspersed throughout FIIS are 17 pre-existing residential communities that in summer months greatly increase in population through the arrival of summer residents and vacationers; in addition, the National Park Service (NPS) has established several facilities on the island to accommodate visitors to FIIS. The 2.2 million people estimated by the NPS to visit Fire Island annually impact groundwater quality through the release of waste-derived contaminants, such as nutrients, pathogens, and organic compounds, into the environment. Waste-contaminated groundwater can move through the aquifer and threaten the ecological health of the adjacent back-barrier estuaries to which much of the groundwater ultimately discharges. In 2004, the U.S. Geological Survey (USGS), in cooperation with the NPS, began a 3-year investigation to (1) collect groundwater levels and water-quality (nutrient) samples, (2) develop a three-dimensional model of the shallow (water-table) aquifer system and adjacent marine surface waters, and (3) calculate nitrogen loads in simulated groundwater discharges from the aquifer to back-barrier estuaries and the ocean. The hydrogeology of the shallow aquifer system was characterized from the results of exploratory drilling, geophysical surveying, water-level monitoring, and water-quality sampling. The investigation focused on four areas-the communities of Kismet and Robbins Rest, the NPS Visitor Center at Watch Hill, and the undeveloped Otis Pike Fire Island High Dune Wilderness. Thirty-five observation wells were installed within FIIS to characterize subsurface hydrogeology and establish a water-table monitoring network in the four study areas. A variable-density model of the shallow aquifer system and adjacent marine surface waters was developed to simulate groundwater flow patterns and rates. Nitrogen loads from the shallow aquifer system were calculated from representative total nitrogen (TN) concentrations and simulated groundwater discharges to back-barrier estuaries and the ocean. The model simulates groundwater directions, velocities, and discharge rates under 2005 mean annual conditions. Groundwater budgets were developed for recharge areas of similar land use that contribute freshwater to back-barrier estuaries, the ocean, and subsea-discharge zones. Total freshwater discharge from the shallow aquifer system is about 43,500 cubic meters per day (m3/d) (79.8 percent) to back-barrier estuaries and about 10,200 m3/d (18.7 percent) to the ocean; about 836 m3/d (1.5 percent) may exit the system as subsea underflow. The total contribution of fresh groundwater to shoreline discharge zones amounts to about 53,700 m3/d (98.5 percent). The median age of freshwater discharged to back-barrier estuaries and the ocean was 3.4 years, and the 95th-percentile age was 20 years. The TN concentrations and loads under 2005 mean annual conditions for areas that contribute fresh groundwater to back-barrier estuaries and the ocean were calculated for the principal land uses on Fire Island. The overall TN load from the shallow aquifer system to shoreline discharge zones is about 16,200 kilograms per year (kg/yr) (82.2 percent) to back-barrier estuaries and about 3,500 kg/yr (17.8 percent) to the ocean. The overall TN load to marine surface waters amounts to about 19,700 kg/yr-roughly 6 percent of the annual TN load from shallow groundwater entering the South Shore Estuary Reserve (SSER) from the Suffolk County mainland, which is about 345,000 kg/yr. In contrast to the TN load from shallow groundwater for the SSER watershed, which annually yields about 353 kilograms per square kilometer (kg/km2), the overall TN loa

  12. TnpI recombinase: identification of sites within Tn5401 required for TnpI binding and site-specific recombination.

    PubMed Central

    Baum, J A

    1995-01-01

    The Bacillus thuringiensis class II transposon Tn5401 encodes a recombinase protein, TnpI, that mediates the resolution of cointegrate molecules generated as intermediates during Tn5401 transposition by the TnpA transposase. This recombination event requires a specific target site, or internal resolution site, at which TnpI binds and catalyzes the exchange of DNA strands. Gel mobility shift assays and DNase I footprinting analyses were used to localize the TnpI binding region to the sequence extending from nucleotides 637 to 747 of Tn5401. Deletions within this region blocked TnpI-mediated recombination in vivo. The 12-bp sequence ATGTCC RCTAAY, present in four copies within the TnpI binding region, is proposed to be the recognition sequence for TnpI binding. TnpI also binds to a single copy of this sequence located within the 53-bp terminal inverted repeats of Tn5401. The unique juxtaposition of recombinase and transposase binding sites at the terminal inverted repeats of Tn5401 suggests that TnpI regulates the binding and/or catalytic activity of TnpA transposase. PMID:7608077

  13. Hydrologic conditions: Dade County, Florida

    USGS Publications Warehouse

    Kohout, Francis Anthony; Klein, Howard; Sherwood, C.B.; Leach, Stanley D.

    1964-01-01

    Thin layers of dense limestone of low permeability that occur near the top of the Biscayne aquifer in the vicinity of the north end of Levee 30 in Dade County, Florida are of hydrologic importance because they retard the downward infiltration of ponded water in Conservation Area No. 3. This retarding effect frequently results in high head differentials across the levee. Tests made in a small area adjacent to Levee 30 indicate that the coefficient of transmissibility of the aquifer is 3,600,000 gpd (gallons per day) per foot, and the coefficient of vertical permeability of the dense limestones is 13 gpd per square foot. If ground-water flow beneath the levee is laminar, the total inflow to the Levee 30 Canal from Conservation Area No. 3 will be about 350 mgd (million gallons per day), or 540 cfs (cubic feet per second), per mile length of levee when the head difference across the levee is 10 feet.

  14. Costilla County Biodiesel Pilot Project

    SciTech Connect

    Doon, Ben; Quintana, Dan

    2011-08-25

    The Costilla County Biodiesel Pilot Project has demonstrated the compatibility of biodiesel technology and economics on a local scale. The project has been committed to making homegrown biodiesel a viable form of community economic development. The project has benefited by reducing risks by building the facility gradually and avoiding large initial outlays of money for facilities and technologies. A primary advantage of this type of community-scale biodiesel production is that it allows for a relatively independent, local solution to fuel production. Successfully using locally sourced feedstocks and putting the fuel into local use emphasizes the feasibility of different business models under the biodiesel tent and that there is more than just a one size fits all template for successful biodiesel production.

  15. County Agents for Children. Final Report. Part I.

    ERIC Educational Resources Information Center

    George Peabody Coll. for Teachers, Nashville, TN. John F. Kennedy Center for Research on Education and Human Development.

    The document, over two-thirds of which consists of attachments, presents Part I of the final report on a child advocacy research and demonstration program in Tennessee, called County Agents for Children. The program established four county agents as general advocates for children in four rural counties, with roles modeled after those of county…

  16. 7 CFR 7.10 - Conduct of county convention.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...2011-01-01 false Conduct of county convention. 7.10 Section 7.10 Agriculture...COMMITTEES § 7.10 Conduct of county convention. (a) The county committee serving...designating the place at which the county convention will be held and for the conduct...

  17. 7 CFR 7.10 - Conduct of county convention.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...2010-01-01 false Conduct of county convention. 7.10 Section 7.10 Agriculture...COMMITTEES § 7.10 Conduct of county convention. (a) The county committee serving...designating the place at which the county convention will be held and for the conduct...

  18. Tri-county pilot study. [Texas

    NASA Technical Reports Server (NTRS)

    Reeves, C. A. (principal investigator); Austin, T. W.; Kerber, A. G.

    1976-01-01

    The author has identified the following significant results. An area inventory was performed for three southeast Texas counties (Montgomery, Walker, and San Jacinto) totaling 0.65 million hectares. The inventory was performed using a two level hierarchy. Level 1 was divided into forestland, rangeland, and other land. Forestland was separated into Level 2 categories: pine, hardwood, and mixed; rangeland was not separated further. Results consisted of area statistics for each county and for the entire study site for pine, hardwood, mixed, rangeland, and other land. Color coded county classification maps were produced for the May data set, and procedures were developed and tested.

  19. Lea County 001, an H5 chondrite, and Lea County 002, an ungrouped type 3 chondrite

    SciTech Connect

    Zolensky, M.E.; Score, R.; Clayton, R.N.; Mayeda, T.K.; Schutt, J.W. (NASA, Johnson Space Center, Houston, TX (USA) Lockheed Engineering and Sciences Co., Houston, TX (USA) Chicago Univ., IL (USA))

    1989-12-01

    A search of active deflation basins near Jal, Lea County, New Mexico resulted in the discovery of two meteorites, Lea County 001 and 002. Lea County 001 has mean olivine and low-Ca pyroxene compositions of Fa(19) and Fs(17), respectively. These and all other mineralogical and petrological data collected indicate a classification of H5 for this stone. Lea County 002 has mean olivine and low-Ca pyroxene compositions of Fa(2) and Fs(4), and is unequilibrated. Although it is mineralogically most similar to Kakangari and chondritic clasts within Cumberland Falls, the high modal amount of forsterite makes Lea County a unique type 3 chondrite. Oxygen isotope data for Lea County 002 fall on an 0-16-mixing line through those of the enstatite meteorites and IAB irons, a feature shared by Kakangari. 28 refs.

  20. The chromate-inducible chrBACF operon from the transposable element TnOtChr confers resistance to chromium(VI) and superoxide.

    PubMed

    Branco, Rita; Chung, Ana Paula; Johnston, Tatiana; Gurel, Volkan; Morais, Paula; Zhitkovich, Anatoly

    2008-11-01

    Large-scale industrial use of chromium(VI) has resulted in widespread contamination with carcinogenic chromium(VI). The abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. Here we report identification of the transposon-located (TnOtChr) chromate resistance genes from the highly tolerant strain Ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mM. The 7,189-bp-long TnOtChr of the mixed Tn21/Tn3 transposon subfamily contains a group of chrB, chrA, chrC, and chrF genes situated between divergently transcribed resolvase and transposase genes. The chrB and chrA genes, but not chrF or chrC, were essential for establishment of high resistance in chromium-sensitive O. tritici. The chr promoter was strongly induced by chromate or dichromate, but it was completely unresponsive to Cr(III), oxidants, sulfate, or other oxyanions. Plasmid reporter experiments identified ChrB as a chromate-sensing regulator of chr expression. Induction of the chr operon suppressed accumulation of cellular Cr through the activity of a chromate efflux pump encoded by chrA. Expression of chrB, chrC, or chrF in an Escherichia coli sodA sodB double mutant restored its aerobic growth in minimal medium and conferred resistance to superoxide-generating agents menadione and paraquat. Nitroblue tetrazolium staining on native gels showed that ChrC protein had superoxide dismutase activity. TnOtChr appears to represent a mobile genetic system for the distribution of the chromate-regulated resistance operon. The presence of three genes protecting against superoxide toxicity should provide an additional survival advantage to TnOtChr-containing cells in the environments with multiple redox-active contaminants. PMID:18776016

  1. The Chromate-Inducible chrBACF Operon from the Transposable Element TnOtChr Confers Resistance to Chromium(VI) and Superoxide?

    PubMed Central

    Branco, Rita; Chung, Ana Paula; Johnston, Tatiana; Gurel, Volkan; Morais, Paula; Zhitkovich, Anatoly

    2008-01-01

    Large-scale industrial use of chromium(VI) has resulted in widespread contamination with carcinogenic chromium(VI). The abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. Here we report identification of the transposon-located (TnOtChr) chromate resistance genes from the highly tolerant strain Ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mM. The 7,189-bp-long TnOtChr of the mixed Tn21/Tn3 transposon subfamily contains a group of chrB, chrA, chrC, and chrF genes situated between divergently transcribed resolvase and transposase genes. The chrB and chrA genes, but not chrF or chrC, were essential for establishment of high resistance in chromium-sensitive O. tritici. The chr promoter was strongly induced by chromate or dichromate, but it was completely unresponsive to Cr(III), oxidants, sulfate, or other oxyanions. Plasmid reporter experiments identified ChrB as a chromate-sensing regulator of chr expression. Induction of the chr operon suppressed accumulation of cellular Cr through the activity of a chromate efflux pump encoded by chrA. Expression of chrB, chrC, or chrF in an Escherichia coli sodA sodB double mutant restored its aerobic growth in minimal medium and conferred resistance to superoxide-generating agents menadione and paraquat. Nitroblue tetrazolium staining on native gels showed that ChrC protein had superoxide dismutase activity. TnOtChr appears to represent a mobile genetic system for the distribution of the chromate-regulated resistance operon. The presence of three genes protecting against superoxide toxicity should provide an additional survival advantage to TnOtChr-containing cells in the environments with multiple redox-active contaminants. PMID:18776016

  2. A TALE of Transposition: Tn3-Like Transposons Play a Major Role in the Spread of Pathogenicity Determinants of Xanthomonas citri and Other Xanthomonads

    PubMed Central

    Ferreira, Rafael Marini; de Oliveira, Amanda Carolina P.; Moreira, Leandro M.; Belasque, José; Gourbeyre, Edith; Siguier, Patricia; Ferro, Maria Inês T.; Ferro, Jesus A.

    2015-01-01

    ABSTRACT Members of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomonas citri plasmid pXAC64. These are flanked by short inverted repeats (IRs). The region was identified as a transposon, TnXax1, with typical Tn3 family features, including a transposase and two recombination genes. Two 14-bp palindromic sequences within a 193-bp potential resolution site occur between the recombination genes. Additional derivatives carrying different T3SEs and other passenger genes occur in different Xanthomonas species. The T3SEs include transcription activator-like effectors (TALEs). Certain TALEs are flanked by the same IRs as found in TnXax1 to form mobile insertion cassettes (MICs), suggesting that they may be transmitted horizontally. A significant number of MICs carrying other passenger genes (including a number of TALE genes) were also identified, flanked by the same TnXax1 IRs and delimited by 5-bp target site duplications. We conclude that a large fraction of T3SEs, including individual TALEs and potential pathogenicity determinants, have spread by transposition and that TnXax1, which exhibits all of the essential characteristics of a functional transposon, may be involved in driving MIC transposition. We also propose that TALE genes may diversify by fork slippage during the replicative Tn3 family transposition. These mechanisms may play a crucial role in the emergence of Xanthomonas pathogenicity. PMID:25691597

  3. Welcome to Union County Extension. The ser-vice offered to the citizens of Union County is a

    E-print Network

    Jawitz, James W.

    Welcome to Union County Extension. The ser- vice offered to the citizens of Union County is a partnership between Union County Board of County Commissioners and the University of Florida/IFAS. The mission we provide by visiting us at our website: http:// union.ifas.ufl.edu. Basil Bactawar, Director Union

  4. 69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C.L. Wood, the county engineer. Updated through the mid-1930s to show new federal aid-state roads. Compares modern system with older county system. Original scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

  5. DUST TRANSPORT IN MARICOPA COUNTY, ARIZONA

    EPA Science Inventory

    Numerical simulations have been carried out for ambient air supermicrometric particulate concentrations in Maricopa County, Arizona during late fall and winter periods of atmospheric stability. Results of model studies are in approximate agreement with limited field observational...

  6. 29 CFR 780.812 - “County

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...UNDER THE FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) County...

  7. 29 CFR 780.812 - “County

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...UNDER THE FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) County...

  8. 29 CFR 780.812 - “County

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...UNDER THE FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) County...

  9. 29 CFR 780.812 - “County

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...UNDER THE FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) County...

  10. 29 CFR 780.812 - “County

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...UNDER THE FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) County...

  11. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    24 Housing and Urban Development 3 2011-04-01 2010-04-01 true Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE...

  12. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    24 Housing and Urban Development 3 2014-04-01 2013-04-01 true Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE...

  13. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued)...

  14. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued)...

  15. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    24 Housing and Urban Development 3 2012-04-01 2012-04-01 false Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued)...

  16. Burleson County State of the Community Report

    E-print Network

    Cousins, Tiffany; English, Jeffrey; Meng, Yi

    2015-01-01

    “Although bears, alligators, and buffaloes once roamed the area they disappeared in the nineteenth century, the county is still inhabited by many wild animal species, including white-tailed deer, coyotes, skunks, raccoons, and opossums, and such wild...

  17. Liberty County State of the Community Report

    E-print Network

    Perkes, Scott; Deng, Sitong; Rojas, Ricardo J. Maga

    2015-01-01

    , Hickories, Bald cypress, Spanish moss, Water lilies, Purple bladderwort, Prickly pear cactus, Yucca, Texas trailing phlox and etc. The Sam Houston National Forest (near Huntsville) is also located in Liberty County and contains many kinds of vegetation...

  18. Edwards Aquifer Evaluation: Kinney County, Texas

    NASA Astrophysics Data System (ADS)

    Khorzad, Kaveh

    2003-10-01

    The Edwards Aquifer is one of the most studied and most prolific aquifers in the United States. The aquifer is a heavily fractured and faulted carbonate aquifer with transmissivities in excess of 100 ft2/s. The City of San Antonio relies upon the Edwards Aquifer as its sole source for water. Much work has been done on quantifying recharge to the aquifer and discharge from wells and acquiring aquifer characteristics from pumping tests, specific capacity tests, and geophysical logs. Although the aquifer has been well studied in Bexar County, much less is known about the Edwards Aquifer in Kinney County. This is partly due to the lower population within the county (approximately 3,500 people) relative to the eastern counties (Uvalde, Medina, Bexar, Comal, and Hays) and the great distance of Kinney County from high profile discharge areas such as the City of San Antonio and Comal and San Marcos Springs. Three key products resulted from this study: (1) exploratory well drilling and the largest aquifer test in the county that were conducted to evaluate the well yields within a 10,000 acre study area in which a drawdown of 2.5 ft approximately 1.2 miles away was observed while pumping at approximately 4,600 gpm; (2) a recharge estimate for the Edwards Aquifer within Kinney County of approximately 71,382 ac-ft/yr; and (3) locating the Brackettville Groundwater Divide from an evaluation of ground water flow direction and hydrograph analysis. These results help evaluate the complex hydraulics occurring within Kinney County and aid in development of ground water modeling that will be used in managing the Edwards Aquifer.

  19. Purification and structure elucidation of three naturally bioactive molecules from the new terrestrial Streptomyces sp. TN17 strain.

    PubMed

    Smaoui, Slim; Mellouli, Lotfi; Lebrihi, Ahmed; Coppel, Yannick; Fguira, Lilia Fourati Ben; Mathieu, Florence

    2011-04-01

    Thirty litres of fermentation broth was extracted from the newly isolated Streptomyces sp. strain TN17 and various separation and purification steps led to the isolation of three pure bioactive compounds (1-3). Compound 1: cyclo (L-Leu-L-Arg), a diketopiperazine 'DKP' derivative; 2: di-(2-ethylhexyl) phthalate, a phthalate derivative; and 3: cyclo 1-[2-(cyclopentanecarbonyl-3-phenyl-propionyl]-pyrrolidine-2-carboxylic acid (1-carbamoyl-propyl)-amide, a cyclic tetrapeptide derivative. The chemical structure of these three active compounds was established on the basis of spectroscopic studies (MS and NMR) and by comparison with data from the literature. According to our biological studies, the pure compounds (1-3) possess antibacterial and antifungal activities. PMID:21331973

  20. The flora of Oktibbeha County, Mississippi

    USGS Publications Warehouse

    Leidolf, A.; McDaniel, S.; Nuttle, T.

    2002-01-01

    We surveyed the flora of Oktibbeha County, Mississippi, U.S.A., from February 1994 to 1996. Occupying 118 square kilometers in east-central Mississippi, Oktibbeha County lies among 3 physiographic regions that include, from west to east, Interior Flatwoods, Pontotoc Ridge, and Black Prairie. Accordingly, the county harbors a diverse flora. Based on field work, as well as an extensive review of published literature and herbarium records at IBE and MISSA, we recorded a total of 1,148 taxa (1,125 species, 7 hybrids, 16 infraspecific taxa) belonging to 514 genera in 160 families, over 85% of all taxa documented were native. Compared to 3 other counties in east-central Mississippi, Oktibbeha County has the second largest recorded flora. The number of state-listed (endangered, threatened, or of special concern) taxa (67) documented in this survey far exceeds that reported from any other county in the region. Three introduced species, Ilex cornuta Lindl. & Paxton, Mahonia bealei (Fortune) Carrie??re, and Nandina domestica Thunb., are reported in a naturalized state for the first time from Mississippi. We also describe 16 different plant communities belonging to 5 broad habitat categories: bottomland forests, upland forests and prairies, aquatic habitats, seepage areas, and human-influenced habitats. A detailed description of the vegetation associated with each of these communities is provided.

  1. Annual Performance Evaluation of a Pair of Energy Efficient Houses (WC3 and WC4) in Oak Ridge, TN

    SciTech Connect

    Biswas, Kaushik [ORNL; Christian, Jeffrey E [ORNL; Gehl, Anthony C [ORNL; Jackson, Roderick K [ORNL; Boudreaux, Philip R [ORNL

    2012-04-01

    Beginning in 2008, two pairs of energy-saver houses were built at Wolf Creek in Oak Ridge, TN. These houses were designed to maximize energy efficiency using new ultra-high-efficiency components emerging from ORNL s Cooperative Research and Development Agreement (CRADA) partners and others. The first two houses contained 3713 square feet of conditioned area and were designated as WC1 and WC2; the second pair consisted of 2721 square feet conditioned area with crawlspace foundation and they re called WC3 and WC4. This report is focused on the annual energy performance of WC3 and WC4, and how they compare against a previously benchmarked maximum energy efficient house of a similar footprint. WC3 and WC4 are both about 55-60% more efficient than traditional new construction. Each house showcases a different envelope system: WC3 is built with advanced framing featured cellulose insulation partially mixed with phase change materials (PCM); and WC4 house has cladding composed of an exterior insulation and finish system (EIFS). The previously benchmarked house was one of three built at the Campbell Creek subdivision in Knoxville, TN. This house (CC3) was designed as a transformation of a builder house (CC1) with the most advanced energy-efficiency features, including solar electricity and hot water, which market conditions are likely to permit within the 2012 2015 period. The builder house itself was representative of a standard, IECC 2006 code-certified, all-electric house built by the builder to sell around 2005 2008.

  2. The Bridge of Mandolin County

    NASA Astrophysics Data System (ADS)

    Lantz, Juliette M.; Feindt, Jenny E.; Lewellyn, Eric P. B.; Walczak, Mary M.

    1999-12-01

    The Bridge of Mandolin County is a case designed to teach the general chemistry principles of molar mass, ions and aqueous reactions, solubility rules, and inorganic nomenclature. Through the instructor-facilitated class discussion, students consider the options before the Mandolin Town Council regarding deicing the newly constructed bridge connecting Mandolin with a large nearby city. The students must decipher contradictory claims made on behalf of sodium chloride, the traditional deicer, and calcium magnesium acetate, a new environmentally friendly deicer, to arrive at the most cost-effective and environmentally appropriate deicing product. As they work through the analysis they raise questions that can be addressed in a laboratory setting. Four optional role-playing experiments are included, which can be used by the students to gather information helpful to resolution of the case. The case is intended to be used over two class periods, with a laboratory period in between, though suggestions for other models are provided. Laboratory procedures include an EDTA titration for Ca2+ and Mg2+, a gravimetric analysis, a qualitative examination of ions and solubility, an introduction to freezing point depression and measurement, and an experimental design activity. This case can also successfully be used without alteration in non-majors chemistry or environmental chemistry courses, or upper-level analytical or environmental chemistry courses.

  3. ION GNSS+ 2013, Session D1, Nashville, TN, 16-20 September 2013 Page 1/15 Four-constellation Reliability in Challenging

    E-print Network

    Calgary, University of

    ION GNSS+ 2013, Session D1, Nashville, TN, 16-20 September 2013 Page 1/15 Four-constellation Kingdom in 2010. Her research interests include reliability of satellite navigation systems, multi-constellation of ranging signals from new GNSS constellations is expected to result in better system availability

  4. Copyright0 1987 by the GeneticsSocietyof America A Tn10-lacZ-kanR-URA3Gene Fusion Transposon for Insertion

    E-print Network

    Botstein, David

    Copyright0 1987 by the GeneticsSocietyof America A Tn10-lacZ-kanR-URA3Gene Fusion Transposon for Insertion Mutagenesis and Fusion Analysis of Yeast and Bacterial Genes Olivier Huisman,**'Wendy Raymond December 23, 1986 Accepted March 2, 1987 ABSTRACT We describe here a new variant of transposon Tnl

  5. P-130 / F. S. Yeung P-130: Large-Cell-Gap Bistable TN-LCD Based on Dual-Frequency Operation

    E-print Network

    P-130 / F. S. Yeung P-130: Large-Cell-Gap Bistable TN-LCD Based on Dual-Frequency Operation Fion S-paper. There are many LCD configurations that are bistable and possess two stable states under no voltage conditions. Examples include bistable cholesteric LCD, bistable ferroelectric LCD, zenithal bistable display

  6. Organization of the Antiseptic Resistance Gene qacA and Tn552Related  Lactamase Genes in Multidrug- Resistant Staphylococcus haemolyticus Strains of Animal and Human Origins

    Microsoft Academic Search

    I.-L. Anthonisen; M. Sunde; T. M. Steinum; M. S. Sidhu; H. Sorum

    2002-01-01

    A part (12 kb) of a plasmid containing the -lactamase genes of Tn552, the disinfectant resistance gene qacA, and flanking DNA has been cloned from a Staphylococcus haemolyticus isolate and sequenced. This region was used to map the corresponding regions in six other multiresistant S. haemolyticus isolates of human and animal origin. The organizations of the genetic structures were almost

  7. Laparoscopic Image Guidance via Conoscopic Holography R. A. Lathrop, T. T. Cheng, and R. J. Webster III, Vanderbilt Univ., Nashville, TN, USA

    E-print Network

    Webster III, Robert James

    III, Vanderbilt Univ., Nashville, TN, USA Spatially registered 3D preoperative medical images can, Galloway RL, Miga MI, 2003, "Incorporation of a laser range scanner into image-guided liver surgery-of-sight access for laser triangulation (e.g. the system of Pathfinder Therapeutics, Inc.). We present

  8. THE MARKET FOR MODERN ART IN NEW YORK IN THE NINETEEN FORTIFS AND NIN1TN FIFTIES -A SThUCTURAL AND HISTORICAL

    E-print Network

    Blandford, Ann

    THE MARKET FOR MODERN ART IN NEW YORK IN THE NINETEEN FORTIFS AND NIN1TN FIFTIES - A STh-called 'New York School '), also witnessed its developient into a market for modern art, both European of this study identifies the key constituents of the 'support- system' in the New York art market in this period

  9. Progress tn Surface Science, Vol. 59, No. 1-4, pp. 255-264, 1998 0 1998 Elsevier Science Ltd. All rights reserved

    E-print Network

    Deymier, Pierre

    Pergamon Progress tn Surface Science, Vol. 59, No. 1-4, pp. 255-264, 1998 0 1998 Elsevier Science STOP BANDS AND DEFECT MODES IN ONE-DIMENSIONAL WAVEGUIDE WITH DANGLING SIDE BRANCHES B. DJAFARI ROUHANI, Mexico 3Department of Materials Science and Engineering, University of Arizona, Tucson, U.S.A. 4

  10. Directing enhancer-traps and iTol2 end-sequences to deleted BAC ends with loxP- and lox511-Tn10 transposons.

    PubMed

    Chatterjee, Pradeep K

    2015-01-01

    A step-by-step detailed procedure is presented to progressively truncate genomic DNA inserts from either end in BACs. The bacterial transposon Tn10 carrying a loxP or a lox511 site is inserted at random into BAC DNA inside the bacterial cell. The cells are then infected with bacteriophage P1. The Cre protein expressed by phage P1 generates end-deletions by specifically recombining the inserted loxP (or lox511) with the loxP (or lox511) endogenous to and flanking insert DNA in BACs from the respective end. The Cre protein also helps phage P1 transduce the BAC DNA by packaging it in P1 heads. This packaging by P1 not only recovers the rare BAC clones containing Tn10 insertions efficiently but also selects end-truncated BACs from those containing inversions of portions of their DNA caused by transposition of Tn10 in the opposite orientation. The libraries of end-deleted BACs generated by this procedure are suitable for numerous mapping studies. Because DNA in front of the loxP (or lox511) arrowheads in the Tn10 transposon is retained at the newly created BAC end, exogenous DNA cassettes such as enhancer-traps and iTol2 ends can be efficiently introduced into BAC ends for germline expression in zebrafish or mice. The methodology should facilitate functional mapping studies of long-range cis-acting gene regulatory sequences in these organisms. PMID:25239743

  11. 76 FR 4254 - Irish Potatoes Grown in Certain Designated Counties in Idaho, and Malheur County, Oregon...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    ...AMS-FV-10-0109; FV11-945-1] Irish Potatoes Grown in Certain Designated Counties...conducted among eligible producers of Irish potatoes in certain designated counties in Idaho...order regulating the handling of Irish potatoes grown in the production area....

  12. 75 FR 26709 - Clarke County Water Supply Project, Clarke County, IA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ...County Water Supply Project, Clarke County, IA AGENCY: Natural Resources Conservation...Walnut Street, Room 693, Des Moines, IA 50309-2180, telephone: 515-284- 4769...the Iowa NRCS Web site at http://www.ia.nrcs.usda.gov. A map of the...

  13. Clean Fuel Advanced Technology 2014 Grant Awardees Applicant Project Type County(s)

    E-print Network

    Clean Fuel Advanced Technology 2014 Grant Awardees Applicant Project Type County(s) Award Amount, Forsyth, Guilford $200,000 $90,525 $290,525 Greensboro Police Department Two electric Zero Motorcycles for use by the Police Department. Guilford $21,395 $5,349 $26,744 MacLeod Construction Nine CNG concrete

  14. Beginnings of geothermal impact on county population and leadership, Imperial County, California

    SciTech Connect

    Pick, J.B.; Butler, E.W.

    1980-09-01

    A major geothermal energy development scenario is about to begin in Imperial County. Initial energy-socioeconomic interactions in the areas of population and county leadership structure are discussed. These include immigration of energy company workers, a sewage plant dispute, conflict over union affiliation of geothermal laborers, and a transmission corridor routing dispute.

  15. COUNTY REP TIPS Learn what works from other experienced County Reps!

    E-print Network

    Kravets, Robin

    COUNTY REP TIPS Learn what works from other experienced County Reps! Gathering parent contact information 1. Contact the Parent Programs Office- all parents of current U of I students are automatically get-together 2. A local restaurant a. Counter service- reserve an area for about 1 ½ hours, and invite

  16. The State of Texas Children 1994: A County by County Fact Book.

    ERIC Educational Resources Information Center

    Texas Kids Count Project, Austin.

    As part of a nationwide effort to highlight the well-being of children in every state, Texas Kids Count is building a comprehensive database of indicators of child well-being to be used to produce annual data books and other analyses on the status of children in Texas. This report presents, county by county, data on several child well-being…

  17. Lectinochemical studies on the glyco-recognition factors of a Tn (GalNAcalpha1-->Ser/Thr) specific lectin isolated from the seeds of Salvia sclarea.

    PubMed

    Wu, Albert M

    2005-01-01

    The lectin extracted from the seeds of Salvia sclarea (SSL) recognizes the Tn antigen (GalNAc alpha1-->Ser/Thr) expressed in certain human carcinomas. In previous studies, knowledge of the binding properties of SSL was restricted to GalNAcalpha1--> related oligosaccharides and glycopeptides. Thus, the requirements of functional groups in monosaccharide and high-density polyvalent carbohydrate structural units for SSL binding and an updated affinity profile were further evaluated by enzyme-linked lectinosorbent (ELLSA) and inhibition assays. Among the glycoproteins (gps) tested for interaction, a high density of exposed Tn-containing glycoproteins such as in the armadillo salivary Tn glycoprotein and asialo ovine salivary glycoprotein reacted best with SSL. When the gps were tested for inhibition of SSL binding, which was expressed as 50% nanogram inhibition, the high density polyvalent Tn present in macromolecules was the most potent inhibitor. Among the monosaccharide and carbohydrate structural units studied, which were expressed as nanomole inhibition, GalNAc alpha1-->3GalNAc beta1-->3Gal alpha1-->4Gal beta1-->4Glc (Fp), GalNAc alpha1-->3Gal beta1-->4Glc (A(L)), GalNAc alpha1-->3GalNAc beta1-->Me (F beta), GalNAc alpha1-->3GalNAc alpha1-->Me (F alpha) and GalNAc alpha1--> Ser/Thr (Tn) were the most active ligands, being 2.5-5.0 x 10(3) and 1.25-2.5 times more active than Gal and GalNAc, respectively. From the results, it is suggested that the combining site of SSL is a shallow groove type, recognizing the monosaccharide of GalNAc as the major binding site or Tn up to the Forssman pentasaccharide (Fp). It can be concluded that the three critical factors for SSL binding are the -NH CH(3)CO at carbon-2 in Gal, the configuration of carbon-3 in GalNAc, and the polyvalent Tn (GalNAc alpha1-->Ser/Thr) present in macromolecules. These results should assist in understanding the glyco-recognition factors involved in carbohydrate-lectin interactions in biological processes. The effect of the polyvalent F alpha, F beta and GalNAc beta1-->3Gal alpha1--> (P alpha) glycotopes on binding should be examined. However, this is hampered by the lack of availability of suitable reagents. PMID:15864748

  18. Ground water in Creek County, Oklahoma

    USGS Publications Warehouse

    Cady, Richard Carlysle

    1937-01-01

    Creek County has been designated as a problem area by the Land Use Planning Section of the Resettlement Administration. Some of the earliest oil fields to brought into production were situated in and near this county, and new fields have been opened from time to time during the ensuing years. The production of the newer fields, however, has not kept pace with the exhaustion of the older fields, and the county now presents an excellent picture of the problems involved in adjusting a population to lands that are nearly depleted of their mineral wealth. Values of land have been greatly depressed; tax collection is far in arrears; tenancy is widespread; and in addition more people will apparently be forced to depend on the income from agriculture than the land seems capable of supporting. The county as a whole is at best indifferently suitable for general farming. The Land Use planning Section proposes to study the present and seemingly immanent maladjustments of population to the resources of the land, and make recommendations for their correction. The writer was detailed to the Land Use Planning Section of Region VIII for the purposes of making studies of ground water problems in the region. In Creek County two investigations were made. In September, 1936, the writer spent about ten days investigating the availability of ground water for the irrigation of garden crops during drouths. If it proved feasible to do this generally throughout the county, the Land Use Planning Section might be able to encourage this practice. The second investigation made by the writer was in regard to the extent to which ground water supplies have been damaged by oil well brines. He was in county for four days late in January 1937, and again in March, 1937. During part of the second field trip he was accompanied by R.M. Dixon, sanitary engineer of the Water Utilization Unit of the Resettlement Administration. (available as photostat copy only)

  19. 77 FR 12830 - Pershing County Water Conservation District; Notice of Intent To File License Application, Filing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-02

    ...14327-000] Pershing County Water Conservation District; Notice of Intent...Submitted by: Pershing County Water Conservation District (Pershing County...Bennie Hodges, Pershing County Water Conservation District, P.O. Box...

  20. 76 FR 71936 - Upper Deckers Creek Watershed, Preston County, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-21

    ...Conservation Service Upper Deckers Creek Watershed, Preston County, WV AGENCY: Natural...prepared for the Upper Deckers Creek Watershed, Preston County, West Virginia. FOR...Domestic Assistance under No. 10.904--Watershed Protection and Flood...

  1. 75 FR 22892 - Environmental Impact Statement: Salt Lake County, UT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ...Administration Environmental Impact Statement: Salt Lake County, UT AGENCY: Federal Highway...proposed transportation improvement project in Salt Lake County, Utah. FOR FURTHER INFORMATION...Administration, 2520 West 4700 South, Suite 9A, Salt Lake City, UT 84118, telephone...

  2. 75 FR 9476 - Environmental Impact Statement: Salt Lake County, UT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ...Administration Environmental Impact Statement: Salt Lake County, UT AGENCY: Federal Highway...proposed transportation improvement project in Salt Lake County, Utah. FOR FURTHER INFORMATION...Administration, 2520 West 4700 South, Suite 9A, Salt Lake City, UT 84118, Telephone:...

  3. 11. ROAD VIEW. SOUTH OF PIERCY, HUMBOLDT COUNTY, CALIFORNIA. OLD ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. ROAD VIEW. SOUTH OF PIERCY, HUMBOLDT COUNTY, CALIFORNIA. OLD HIGHWAY ON LEFT. NEW HIGHWAY ON RIGHT. LOOKING SW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  4. 15. HIGHWAY 101 BRIDGE IN RICHARDSON STATE PARK. HUMBOLDT COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. HIGHWAY 101 BRIDGE IN RICHARDSON STATE PARK. HUMBOLDT COUNTY, CALIFORNIA. LOOKING N. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  5. 67. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    67. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  6. 25. ROAD VIEW ON NEWTON DRURY PARKWAY. HUMBOLDT COUNTY, CALIFORNIA. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. ROAD VIEW ON NEWTON DRURY PARKWAY. HUMBOLDT COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  7. 24. ROAD VIEW AT PRAIRE CREEK STATE PARK. HUMBOLDT COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. ROAD VIEW AT PRAIRE CREEK STATE PARK. HUMBOLDT COUNTY, CALIFORNIA. LOOKING N. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  8. 17. BENBOW INN. SOUTH OF GARBERVILLE, HUMBOLDT COUNTY, CALIFORNIA. LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. BENBOW INN. SOUTH OF GARBERVILLE, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  9. 2. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  10. 10. ROAD VIEW. SOUTH OF PIERCY, HUMBOLDT COUNTY, CALIFORNIA. OLD ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. ROAD VIEW. SOUTH OF PIERCY, HUMBOLDT COUNTY, CALIFORNIA. OLD HIGHWAY ON LEFT. NEW HIGHWAY ON RIGHT. LOOKING W. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  11. 19. OLD HIGHWAY 101 AND ROAD VIEW. MIRANDA, HUMBOLDT COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. OLD HIGHWAY 101 AND ROAD VIEW. MIRANDA, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  12. Photocopy of drawing (original In possession of Allegany County, Cumberland, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of drawing (original In possession of Allegany County, Cumberland, MD) WELL WATER RESERVOIR, 1926 - Kelly-Springfield Tire Plant, Cooling Tower, 701 Kelly Road, Cumberland, Allegany County, MD

  13. 76 FR 12316 - El Dorado County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ...DEPARTMENT OF AGRICULTURE Forest Service El Dorado County Resource Advisory Committee...SUMMARY: The El Dorado County Resource Advisory Committee...ADDRESSES: The meeting will be held at the El Dorado Center of Folsom Lake College,...

  14. 75 FR 59680 - El Dorado County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-28

    ...DEPARTMENT OF AGRICULTURE Forest Service El Dorado County Resource Advisory Committee...SUMMARY: The El Dorado County Resource Advisory Committee...ADDRESSES: The meeting will be held at the El Dorado Center of Folsom Lake College,...

  15. 75 FR 32739 - El Dorado County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-09

    ...DEPARTMENT OF AGRICULTURE Forest Service El Dorado County Resource Advisory Committee...SUMMARY: The El Dorado County Resource Advisory Committee...ADDRESSES: The meeting will be held at the El Dorado Center of Folsom Lake College,...

  16. 77 FR 49776 - El Dorado County Resource Advisory COMMITTEE

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-17

    ...DEPARTMENT OF AGRICULTURE Forest Service El Dorado County Resource Advisory COMMITTEE...SUMMARY: The El Dorado County Resource Advisory Committee...Community Room at Folsom Lake College, El Dorado Center, 6699 Campus [[Page...

  17. 76 FR 24851 - El Dorado County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-03

    ...DEPARTMENT OF AGRICULTURE Forest Service El Dorado County Resource Advisory Committee...SUMMARY: The El Dorado County Resource Advisory Committee...ADDRESSES: The meeting will be held at the El Dorado Center of Folsom Lake College,...

  18. 76 FR 2081 - El Dorado County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-12

    ...DEPARTMENT OF AGRICULTURE Forest Service El Dorado County Resource Advisory Committee...SUMMARY: The El Dorado County Resource Advisory Committee...ADDRESSES: The meeting will be held at the El Dorado Center of Folsom Lake College,...

  19. 76 FR 53113 - El Dorado County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-25

    ...DEPARTMENT OF AGRICULTURE Forest Service El Dorado County Resource Advisory Committee...SUMMARY: The El Dorado County Resource Advisory Committee...ADDRESSES: The meeting will be held at the El Dorado Center of Folsom Lake College,...

  20. 23. Photocopy of photograph (from the Bucks County Historical Society) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Photocopy of photograph (from the Bucks County Historical Society) photographer and date unknown VIEW FROM THE SOUTHWEST - Fonthill, East Court Street, State Route 313 vicinity, Doylestown, Bucks County, PA

  1. 20. Photocopy of photograph (from the Bucks County Historical Society) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Photocopy of photograph (from the Bucks County Historical Society) photographer and date unknown GENERAL VIEW FROM THE SOUTHWEST - Moravian Pottery & Tile Works, Southwest side of State Route 313 (Swamp Road), Northwest of East Court Street, Doylestown, Bucks County, PA

  2. 12. Photocopy of map from Atlas of Westchester County by ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Photocopy of map from Atlas of Westchester County by Joseph E. Bien, published by Julius Bien & Company, New York, 1893 - Moquette Row Housing, Moquette Row North & Moquette Row South, Yonkers, Westchester County, NY

  3. Small County: An Interactive Exercise in Petroleum Exploration Geology

    NSDL National Science Digital Library

    Geoff Bohling

    2010-01-01

    The Small County project provides students with interactive instruction in the geological aspects of petroleum reservoir characterization employing a virtual subsurface closely reflecting the geological characteristics of the US mid-continent, in the fictional setting of Small County, Kansas.

  4. 1. BEGINNING OF RAILWAY SUPERSTRUCTURE IN PHILADELPHIA COUNTY. FACING EAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. BEGINNING OF RAILWAY SUPERSTRUCTURE IN PHILADELPHIA COUNTY. FACING EAST DOWN MARKET STREET. - Market Street Elevated Railway, Market Street between Sixty-ninth & Forty-sixth Streets, Philadelphia, Philadelphia County, PA

  5. 7 CFR 1230.606 - Farm Service Agency County Committee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...2010-01-01 false Farm Service Agency County Committee... AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND...PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures for the Conduct...Definitions § 1230.606 Farm Service Agency County Committee....

  6. 7 CFR 1220.604 - Farm Service Agency County Committee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...2010-01-01 false Farm Service Agency County Committee... AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND...PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum...Definitions § 1220.604 Farm Service Agency County Committee....

  7. 5. TUNNEL TREE AT DRIVETHROUGHTREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. TUNNEL TREE AT DRIVE-THROUGH-TREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NE. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  8. 9. Photocopy of lithograph (from Historical Atlas of Solano County, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Photocopy of lithograph (from Historical Atlas of Solano County, p. 25) H. Strauss, delineator published 1878 'U.S. ARSENAL, BENICIA, CAL.' VIEW FROM SOUTHWEST - Benicia Arsenal, Benicia Industrial Park, Benicia, Solano County, CA

  9. 4. VIEW OF SILVER BRIDGE (ST. MARY'S BRIDGE), CARRYING COUNTY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW OF SILVER BRIDGE (ST. MARY'S BRIDGE), CARRYING COUNTY ROAD OVER SOURIS RIVER NEAR SOUTH END OF REFUGE, LOOKING NORTHEAST - Upper Souris National Wildlife Refuge Dams, Souris River Basin, Foxholm, Ward County, ND

  10. Los Angeles County Poor Farm, Patient Wards 201, 202, 203, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Los Angeles County Poor Farm, Patient Wards 201, 202, 203, 204, 205, 206, 207, 208 & 209 - Type A Plan, 7601 Imperial Highway; bounded by Esperanza Street, Hawthorn Avenue, Laurel Street, and Descanso Street, Downey, Los Angeles County, CA

  11. Los Angeles County Poor Farm, Patient Ward Nos. 210 & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Los Angeles County Poor Farm, Patient Ward Nos. 210 & 211 - Type B Plan, 7601 Imperial Highway; bounded by Esperanza Street, Laurel Street, Flores Street, and Descanso Street, Downey, Los Angeles County, CA

  12. 22. Historic American Buildings Survey Alameda County Recorder Office Map ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Historic American Buildings Survey Alameda County Recorder Office Map Book 6, page 17 October 1960 SURVEY OF 1868 - Mission San Jose de Guadalupe, Mission & Washington Boulevards, Fremont, Alameda County, CA

  13. Energy Efficient Buildings, Salt Lake County, Utah

    SciTech Connect

    Barnett, Kimberly

    2012-04-30

    Executive Summary Salt Lake County's Solar Photovoltaic Project - an unprecedented public/private partnership Salt Lake County is pleased to announce the completion of its unprecedented solar photovoltaic (PV) installation on the Calvin R. Rampton Salt Palace Convention Center. This 1.65 MW installation will be one the largest solar roof top installations in the country and will more than double the current installed solar capacity in the state of Utah. Construction is complete and the system will be operational in May 2012. The County has accomplished this project using a Power Purchase Agreement (PPA) financing model. In a PPA model a third-party solar developer will finance, develop, own, operate, and maintain the solar array. Salt Lake County will lease its roof, and purchase the power from this third-party under a long-term Power Purchase Agreement contract. In fact, this will be one of the first projects in the state of Utah to take advantage of the recent (March 2010) legislation which makes PPA models possible for projects of this type. In addition to utilizing a PPA, this solar project will employ public and private capital, Energy Efficiency and Conservation Block Grants (EECBG), and public/private subsidized bonds that are able to work together efficiently because of the recent stimulus bill. The project also makes use of recent changes to federal tax rules, and the recent re-awakening of private capital markets that make a significant public-private partnership possible. This is an extremely innovative project, and will mark the first time that all of these incentives (EECBG grants, Qualified Energy Conservation Bonds, New Markets tax credits, investment tax credits, public and private funds) have been packaged into one project. All of Salt Lake County's research documents and studies, agreements, and technical information is available to the public. In addition, the County has already shared a variety of information with the public through webinars, site tours, presentations, and written correspondence.

  14. Day Fire in Ventura County

    NASA Technical Reports Server (NTRS)

    2006-01-01

    [figure removed for brevity, see original site] Annotated Version

    The Day fire has been burning in Ventura County in Southern California since Labor Day, and has consumed more than 160,000 acres. As of September 29, it was 63 percent contained. The Advanced Spaceborne Thermal Emission and Reflection Radiometer on NASA's Terra satellite flew over the fire at 10 p.m. Pacific Time on September 28, and imaged the fire with its infrared camera. The hottest areas of active burning appear as red spots on the image. The blue-green background is a daytime image acquired in June, used as a background to allow firefighters to localize the hot spots.

    With its 14 spectral bands from the visible to the thermal infrared wavelength region, and its high spatial resolution of 15 to 90 meters (about 50 to 300 feet), ASTER images Earth to map and monitor the changing surface of our planet.

    ASTER is one of five Earth-observing instruments launched December 18, 1999, on NASA's Terra satellite. The instrument was built by Japan's Ministry of Economy, Trade and Industry. A joint U.S./Japan science team is responsible for validation and calibration of the instrument and the data products.

    The broad spectral coverage and high spectral resolution of ASTER provides scientists in numerous disciplines with critical information for surface mapping, and monitoring of dynamic conditions and temporal change. Example applications are: monitoring glacial advances and retreats; monitoring potentially active volcanoes; identifying crop stress; determining cloud morphology and physical properties; wetlands evaluation; thermal pollution monitoring; coral reef degradation; surface temperature mapping of soils and geology; and measuring surface heat balance.

    The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission directorate.

    Size: 22.5 by 31.0 kilometers (12.6 by 15.2 miles) Location: 34.6 degrees North latitude, 119.1 degrees West longitude Orientation: North at top Image Data: ASTER Bands 4, 2, and 1 Original Data Resolution: ASTER 15 meters (49.2 feet) and 30 meters (98.4 feet) Dates Acquired: September 28, 2006 and June 19 2006

  15. 70. MISSISSIPPI, LOWNDES CO., NORTHWEST CORNER MAP OF LOWNDES COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    70. MISSISSIPPI, LOWNDES CO., NORTHWEST CORNER MAP OF LOWNDES COUNTY, 1931 Detail of ROAD OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C. L. wood, county engineer. Detail of section NW of Columbus (12 mi. N, 9 mi. W), including Plymouth, Waverly, Barton, Buttahatchie R. Orig. scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

  16. 71. MISSISSIPPI, LOWNDES CO. COLUMBUSSTEENS MAP OF LOWNDES COUNTY, 1931 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    71. MISSISSIPPI, LOWNDES CO. COLUMBUS-STEENS MAP OF LOWNDES COUNTY, 1931 Detail of ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C. L. wood, county engineer. Orig. scale: I in. to I mi. Includes Columbus and area NE to steens. Extent: 12 mi. East-West, 9 mi. North-South. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

  17. Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

    SciTech Connect

    Gunderson, Leonard L., E-mail: gunderson.leonard@mayo.edu [Mayo Clinic Cancer Center, Scottsdale, Arizona (United States); Moughan, Jennifer [Radiation Therapy Oncology Group, Philadelphia, Pennsylvania (United States); Ajani, Jaffer A. [The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Pedersen, John E. [Cross Cancer Institute, Edmonton, Alberta (Canada); Winter, Kathryn A. [Radiation Therapy Oncology Group, Philadelphia, Pennsylvania (United States); Benson, Al B. [Northwestern University, Chicago, Illinois (United States); Thomas, Charles R. [Knight Cancer Institute/Oregon Health and Science University, Portland, Oregon (United States); Mayer, Robert J. [Dana-Farber Cancer Institute, Boston, Massachusetts (United States); Haddock, Michael G. [Mayo Clinic Cancer Center, Rochester, Minnesota (United States); Rich, Tyvin A. [University of Virginia, Charlottesville, Virginia (United States); Willett, Christopher G. [Duke University, Durham, North Carolina (United States)

    2013-11-15

    Purpose: The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials: DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the log–rank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray's test. Results: Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions: TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories.

  18. THE ECONOMY OF MANATEE AND SARASOTA COUNTIES Effie Philippakos

    E-print Network

    Florida, University of

    i THE ECONOMY OF MANATEE AND SARASOTA COUNTIES By Effie Philippakos Alan W. Hodges David Mulkey Charles M. Adams Abstract This report is intended to characterize the economies of Manatee and Sarasota-county region. The overall size and seasonal variations in the economies of Manatee and Sarasota Counties were

  19. University of Maryland Baltimore County Office of Sponsored Programs

    E-print Network

    Adali, Tulay

    University of Maryland Baltimore County Office of Sponsored Programs SUBRECPIENT AND VENDOR. Will the University of Maryland Baltimore County be able to complete reports about the project without that is unique to the project and minimal supervision from the PI at the University of Maryland Baltimore County

  20. Impact of Extension in Shawano County. 1. Conclusions & Implications.

    ERIC Educational Resources Information Center

    Forest, Laverne B.; Marshall, Mary G.

    Two-thirds of the 33,000 residents of Shawano County, Wisconsin, live in rural areas; agricultural interests comprise 30% of the employment economy. Inventories of Extension programs from 1960 to 1975 and telephone interviews with county and community leaders and a random sample of 1192 county residents yielded data for this survey. Most adults…

  1. A Plan of Library Services for Pinellas County, Florida.

    ERIC Educational Resources Information Center

    Summers, F. William; Curran, Charles C.

    In order to determine the feasibility of establishing a county-wide library system, the Pinellas County (Florida) Board of County Commissioners authorized a study of existing library services and possible alternatives for reorganization. Data were gathered and analyzed from: (1) questionnaires sent to library directors; (2) interviews with these…

  2. Barton County Community College Transfer Program to University of Kansas

    E-print Network

    Barton County Community College Transfer Program to University of Kansas B.S. Mechanic Engineering for the degree. KU Course Requirements Barton County Equivalents Goal 1: Critical Thinking and Quantitative from outcome 1 and one from outcome 2 Outcome 1 GE21 #12;Barton County Community College Mechanical

  3. Barton County Community College Transfer Program to University of Kansas

    E-print Network

    Barton County Community College Transfer Program to University of Kansas B.S. in Aerospace for the degree. KU Course Requirements Barton County Equivalents Goal 1: Critical Thinking and Quantitative from outcome 1 and one from outcome 2 Outcome 1 GE21 #12;Barton County Community College Aerospace

  4. continued on pg 2 Barton County Community College

    E-print Network

    continued on pg 2 Barton County Community College TRANSFER PROGRAM TO THE UNIVERSITY OF KANSAS for the courses that will be required for the degree. KU Course Requirements Barton County Equivalents Goal 1 ALGEBRA 5 #12;Barton County Community College ~ Photo Media B.F.A. Updated August 30, 2013 2 MATH 101

  5. Barton County Community College Transfer Program to University of Kansas

    E-print Network

    Barton County Community College Transfer Program to University of Kansas Architecture, Civil for the courses that will be required for the degree. KU Course Requirements Barton County Equivalents Goal 1 Units) -two from outcome 1 and one from outcome 2 Outcome 1 GE21 #12;Barton County Community College

  6. 7. Photocopy of map (from Superior Court of Chatham County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photocopy of map (from Superior Court of Chatham County Land Records, Book 2G, p. 299) John McKinnon (County Surveyor), delineator 11 January 1817 PLAT OF SITE, SHOWING EASTERN PORTION OF BUILDING - William Taylor Store, 204 West Bay Street, Savannah, Chatham County, GA

  7. Wheeler County Riparian Buffers; 2002-2003 Annual Report

    Microsoft Academic Search

    Judy Potter; Will Homer

    2004-01-01

    Problems Encountered During Contract Year--Wheeler County residents are mostly non participants when it comes to Farm Services programs. Results of the counties non participation is the rental rates are the lowest in the state. There is a government fear factor as well as an obvious distance limitation. The FSA office is nearly 150 mile roundtrip from two of the counties

  8. Profile of the Minority Population Broome County, New York

    E-print Network

    Suzuki, Masatsugu

    Profile of the Minority Population Broome County, New York Migratory, Demographic, Socio;Profile of the Minority Population of Broome County, New York 1 Preface This report focuses on the Broome not been previously analyzed for Broome County, New York. Thus, while information is dated, it is the most

  9. 27 CFR 9.233 - Kelsey Bench-Lake County.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...section is “Kelsey Bench-Lake County”. For purposes of part...chapter, “Kelsey Bench-Lake County,” “Kelsey Bench...two United States Geological Survey (USGS) 1:24,000 scale...boundary of the Kelsey Bench-Lake County viticultural area...

  10. The Deepwater Horizon oil spill and Miami-Dade County

    E-print Network

    Jawitz, James W.

    The Deepwater Horizon oil spill and Miami-Dade County Issue 8.2 Background On Tuesday, April 20- ida's Gulf Coast shows that values are well below levels of concern for public health. Miami-Dade County It is still too soon to predict if or how Miami-Dade County will be impacted by the oil spill

  11. Santa Clara County Planar Solid Oxide Fuel Cell Demonstration Project

    Microsoft Academic Search

    Fred Mitlitsky; Sara Mulhauser; David Chien; Deepak Shukla; David Weingaertner

    2009-01-01

    The Santa Clara County Planar Solid Oxide Fuel Cell (PSOFC) project demonstrated the technical viability of pre-commercial PSOFC technology at the County 911 Communications headquarters, as well as the input fuel flexibility of the PSOFC. PSOFC operation was demonstrated on natural gas and denatured ethanol. The Santa Clara County Planar Solid Oxide Fuel Cell (PSOFC) project goals were to acquire,

  12. Sharpening the Focus of Yolo County Land Use Policy

    E-print Network

    Ferrara, Katherine W.

    Sharpening the Focus of Yolo County Land Use Policy Kurt R. Richter University of California Agricultural Issues Center October 2009 #12;Sharpening the Focus of Yolo County Land Use Policy II University of California Agricultural Issues Center #12;Sharpening the Focus of Yolo County Land Use Policy III Making

  13. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...2014-04-01 2014-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

  14. 27 CFR 9.169 - Red Hills Lake County.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...2011-04-01 2011-04-01 false Red Hills Lake County. 9.169 Section...American Viticultural Areas § 9.169 Red Hills Lake County. (a) Name. The...viticultural area described in this section is “Red Hills Lake County”. (b) Approved...

  15. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...2012-04-01 2012-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

  16. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...2010-04-01 2010-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

  17. 27 CFR 9.169 - Red Hills Lake County.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...2014-04-01 2014-04-01 false Red Hills Lake County. 9.169 Section...American Viticultural Areas § 9.169 Red Hills Lake County. (a) Name. The...viticultural area described in this section is “Red Hills Lake County”. (b) Approved...

  18. 27 CFR 9.169 - Red Hills Lake County.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...2012-04-01 2012-04-01 false Red Hills Lake County. 9.169 Section...American Viticultural Areas § 9.169 Red Hills Lake County. (a) Name. The...viticultural area described in this section is “Red Hills Lake County”. (b) Approved...

  19. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...2011-04-01 2011-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

  20. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...2013-04-01 2013-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...