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1

Geological and petrophysical characterization of the Ferron Sandstone for 3-D simulation of a fluvial-deltaic reservoir. Deliverable 2.5.4, Ferron Sandstone lithologic strip logs, Emergy & Sevier Counties, Utah: Volume I  

SciTech Connect

Strip logs for 491 wells were produced from a digital subsurface database of lithologic descriptions of the Ferron Sandstone Member of the Mancos Shale. This subsurface database covers wells from the parts of Emery and Sevier Counties in central Utah that occur between Ferron Creek on the north and Last Chance Creek on the south. The lithologic descriptions were imported into a logging software application designed for the display of stratigraphic data. Strip logs were produced at a scale of one inch equals 20 feet. The strip logs were created as part of a study by the Utah Geological Survey to develop a comprehensive, interdisciplinary, and qualitative characterization of a fluvial-deltaic reservoir using the Ferron Sandstone as a surface analogue. The study was funded by the U.S. Department of Energy (DOE) under the Geoscience/Engineering Reservoir Characterization Program.

Allison, M.L.

1995-12-08

2

Extensional collapse along the Sevier Desert reflection, northern Sevier Desert basin, western United States  

NASA Astrophysics Data System (ADS)

Newly released and previously published seismic reflection data from the northern Sevier Desert basin provide a complete seismic transect between the tilted western margin of the basin and the eastern breakaway zone. When tied to well and surface age data, the transect delineates a continuum of extensional fault and basin fill geometries that developed between late Oligocene and Pleistocene time across the basin. A minimum of 18 km of top-to-the-west normal displacement is estimated across the Sevier Desert from only the most conspicuous growth geometries and offsets across listric normal faults that sole downward into the Sevier Desert reflection (SDR). The SDR clearly marks a normal fault zone beneath the entire basin, where stratal truncations are imaged for 50% of the 39 km length of the reflection east of the Cricket Mountains block. Restoration of extensional displacement along this entire 39 km fault length is necessary to reconstruct the pre-Oligocene configuration and erosion level of Sevier thrust sheets across the Sevier Desert area. The SDR normal fault zone underlies the former topographic crest of the Sevier orogenic belt, where it accommodated extensional collapse after cessation of regional contractile tectonism.

Coogan, James C.; Decelles, Peter G.

1996-10-01

3

A Case Study of the Cocke County (TN) School System and Its Role as a Partner in the NSF-Supported Appalachian Rural Systemic Initiative.  

ERIC Educational Resources Information Center

This case study examines the history and current circumstances of education in Cocke County (Tennessee) in the context of its participation in the Appalachian Rural Systemic Initiative (ARSI), which aims to improve science and mathematics achievement through systemic reform. Sections of this report describe the county's history, demography, and…

Horn, Jerry G.; Oliver, Steve; Stufflebeam, Daniel

4

Elkmont Vehicle Bridge, Construction PeelAway Great Smoky Mountains National ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Elkmont Vehicle Bridge, Construction Peel-Away - Great Smoky Mountains National Park Roads & Bridges, Elkmont Vehicle Bridge, Spanning Little River at Elkmont Campground, Gatlinburg, Sevier County, TN

5

19. ELEVATION OF TOWNSEND WYE BRIDGE LOOKING SSW. Great ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. ELEVATION OF TOWNSEND WYE BRIDGE LOOKING SSW. - Great Smoky Mountains National Park Roads & Bridges, Little River Road, Between Sugarlands Visitor Center & Townsend Wye, Gatlinburg, Sevier County, TN

6

OBVERSE VIEW OF TN24911, LOOKING BACK FROM THE NORTHEAST ROOM ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

OBVERSE VIEW OF TN-249-11, LOOKING BACK FROM THE NORTHEAST ROOM TO THE STAIR HALL. THE DOOR TO THE NORTH (DINING) ROOM LIES AS LEFT - Hilderbrand House, 4571 Airways Boulevard, Memphis, Shelby County, TN

7

Inventory of Wetlands and Agricultural Land Cover in the Upper Sevier River Basin, Utah.  

National Technical Information Service (NTIS)

The use of color infrared aerial photography in the mapping of agricultural land use and wetlands in the Sevier River Basin of south central utah is described. The efficiency and cost effectiveness of utilizing LANDSAT multispectral scanner digital data t...

R. A. Jaynes L. D. Clark K. F. Landgraf

1981-01-01

8

Role of crustal thickening and extensional collapse in the tectonic evolution of the Sevier-Laramide orogeny, western United States  

Microsoft Academic Search

The effects of crustal thickening and extensional collapse of continental crust, combined with variations in plate-convergence forces, provide a coherent explanation for evolution of the Cretaceous-early Tertiary Sevier-Laramide orogeny. Crustal thickening of the Sevier-Laramide hinterland region was triggered by the combination of compressive plate-convergence forces and subduction-induced conductive heating of the crust. Eastward progradation of the locus of Sevier-Laramide deformation

Richard F. Livaccari

1991-01-01

9

Superfund Record of Decision (EPA Region 4): Oak Ridge Reservation (USDOE), (Operable Unit 4), Roane County, Oak Ridge, TN. (Third remedial action), September 1991  

SciTech Connect

The Oak Ridge Reservation (ORR) (USDOE) (Operable Unit 4) site is a former uranium isotope processing subsite in Oak Ridge, Roan County, Tennessee. The 1,700-acre K-25 site, which comprises Operable Unit 4 (OU4), is one of several hundred waste disposal sites or areas of contamination at the ORR site requiring Superfund remedial action. Land use in the area is predominantly residential. The storage yards contain 36,000 ninety-gallon drums of stabilized sludge, 29,000 ninety-gallon drums of raw sludge, and 16,000 gallons of raw sludge in tanks, contaminated with mixed radioactive and hazardous wastes. The Record of Decision (ROD) addresses the sludge stored at the storage yards as an interim action to prevent or mitigate releases to the environment. The selected remedial action includes eliminating free liquids in sludge through filter press, thermal drying, or similar methods; repacking dry sludge, followed by onsite storage of containers and processing liquids removed from the sludge through existing treatment facilities.

Not Available

1991-09-19

10

Superfund Record of Decision (EPA Rregion 4): Oak Ridge Reservation (USDOE), (Operable Unit 3), Anderson County, Oak Ridge, TN. (Second remedial action), September 1991  

SciTech Connect

The Oak Ridge Reservation (ORR) (USDOE) (Operable Unit 3) site is an active nuclear weapons component manufacturing facility located in Oak Ridge, Anderson County, Tennessee. The Y-12 plant, which is addressed as Operable Unit 3, is one of several hundred waste disposal sites or areas of contamination at the ORR site requiring Superfund remedial action. The site occupies the upper reaches of East Fork Poplar Creek (EFPC) in Bear Creek Valley. From 1940 to the present, the Y-12 plant has been used to produce nuclear weapons components. From 1955 to 1963, mercury was used in a column-exchange process to separate lithium isotopes. Testing of the three concrete tanks showed that the tank sediment contained mercury, and that contaminated waste is still being discharged into two of the three tanks. The Record of Decision (ROD) focuses on the contaminated sediment in the sedimentation tanks as an interim action. The primary contaminants of concern affecting the sediment are mercury, a metal and radioactive materials. The selected interim remedial action for the site is included.

Not Available

1991-09-19

11

Time of initial thrusting in the Sevier orogenic belt, Idaho-Wyoming and Utah  

NASA Astrophysics Data System (ADS)

Reexamination of the distribution of fossils found in the earliest preserved synorogenic conglomerates within the Sevier thrust belt suggests that initial thrust movement may be no older than Aptian age. This interpretation is corroborated by subsidence analyses of sedimentary sequences lying within and east of the Idaho-Wyoming and Utah thrust belts. A major episode of middle Cretaceous (Aptian Cenomanian) subsidence is interpreted as recording the initiation of thrust loading deformation in the adjacent Sevier orogenic belt. An earlier subsidence event took place during Middle Jurassic time, more than 30 m.y. prior to the Cretaceous event, and may be the result of tectonic events to the west that are unrelated to thrust deformation in the Idaho-Wyoming and Utah thrust belts. We find no verifiable evidence to support previous interpretations that Sevier belt deformation and uplift began in Late Jurassic time.

Heller, P. L.; Bowdler, S. S.; Chambers, H. P.; Coogan, J. C.; Hagen, E. S.; Shuster, M. W.; Winslow, N. S.; Lawton, T. F.

1986-05-01

12

Structural styles of the Guess Creek fault block beneath the Great Smoky thrust sheet, Blount County, Tennessee  

Microsoft Academic Search

A road cut along US 321 N, approximately 1 km NW of Walland, TN, exposes a previously unexposed complexly deformed section of Middle Ordovician clastic wedge [Chickamauga Group, Sevier Shale] sedimentary rocks. It provides an excellent opportunity to analyze both the lithologic assemblages and complex folding and faulting beneath the Great Smoky thrust sheet. Arkosic quartzite of the Lower Cambrian

M. W. Carter; G. L. Davidson; J. A. Heller; R. D. Jr. Hatcher

1993-01-01

13

Relations between hinterland and foreland shortening: Sevier orogeny, central North American Cordillera  

Microsoft Academic Search

The tectonic relations between foreland and hinterland deformation in noncollisional orogens are critical to understanding the overall development of orogens. The classic central Cordilleran foreland fold-and-thrust belt in the United States (Late Jurassic to early Tertiary Sevier belt) and the more internal zones to the west (central Nevada thrust belt) provide data critical to understanding the development of internal and

Wanda J. Taylor; John M. Bartley; Mark W. Martin; John W. Geissman; J. Douglas Walker; Phillip A. Armstrong; Joan E. Fryxell

2000-01-01

14

Time of initial thrusting in the Sevier orogenic belt, Idaho-Wyoming and Utah  

Microsoft Academic Search

Reexamination of the distribution of fossils found in the earliest preserved synorogenic conglomerates within the Sevier thrust belt suggests that initial thrust movement may be no older than Aptian age. This interpretation is corroborated by subsidence analyses of sedimentary sequences lying within and east of the Idaho-Wyoming and Utah thrust belts. A major episode of middle Cretaceous (Aptian Cenomanian) subsidence

P. L. Heller; S. S. Bowdler; H. P. Chambers; J. C. Coogan; E. S. Hagen; M. W. Shuster; N. S. Winslow; T. F. Lawton

1986-01-01

15

Time of initial thrusting in the Sevier orogenic belt, Idaho-Wyoming and Utah  

Microsoft Academic Search

Reexamination of the distribution of fossils found in the earliest preserved synorogenic conglomerates within the Sevier thrust belt suggests that initial thrust movement may be no older than Aptian age. This interpretation is corroborated by subsidence analyses of sedimentary sequences lying within and east of the Idaho-Wyoming and Utah thrust belts. A major episode of middle Cretaceous (Aptian-Cenomanian) subsidence is

P. L. Heller; S. S. Bowdler; H. P. Chambers; J. C. Coogan; E. S. Hagen; M. W. Shuster; N. S. Winslow; T. F. Lawton

1986-01-01

16

12. Deck view of bridge near Alum Cave parking area ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Deck view of bridge near Alum Cave parking area looking S. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

17

Mercury Resistance Determinants Related to Tn21, Tn1696, and Tn5053 in Enterobacteria from the Preantibiotic Era  

PubMed Central

Three mer transposons from the Murray collection of preantibiotic enterobacteria show >99% sequence identity to current isolates. Tn5073 is most closely related to Tn5036 and Tn1696, and Tn5074 is most closely related to Tn5053. Tn5075 is most closely related to Tn21 but lacks integron In2 and is flanked by insertion elements.

Essa, Ashraf M. M.; Julian, Daniel J.; Kidd, Stephen P.; Brown, Nigel L.; Hobman, Jon L.

2003-01-01

18

Influence of preexisting tectonic trends on geometries of Sevier orogenic belt and its foreland in Utah  

SciTech Connect

The tectonic style of the late Mesozoic Sevier orogenic belt in Utah was greatly affected by preexisting structural trends that date from the late Precambrian rifting and fragmentation of the North American continent. The late Precambrian cratonic margin (Cordilleran hinge line) was marked by a system of prominent faults including the north-south-trending ancestral Wasatch and ancient Ephraim faults and the southwest-northeast-trending Leamington, Scipio, Cove Fort, and Paragonah faults. During the Paleozoic and Mesozoic, renewed activity on these faults affected the geometries of the late Paleozoic Paradox and Oquirrh basins, the boundaries of the Jurassic Arapien Formation, and the sedimentary pattern of the Cretaceous foreland basin. Many of these fault zones were reactivated as tectonic ramps (e.g., the ancient Ephraim fault) and tear faults (e.g., the Leamington fault) during the compressional Sevier tectonism. The Fillmore arch and some other structural highs situated along the edge of the late Precambrian craton caused ramping of the inner Keystone-Pavant-Canyon thrust sheets and telescoping of the frontal thrust sheets. Post-thrust uplift of basement highs led to tectonic denudation and to the development of low-angle, extensional faults, such as the Sevier detachment. Northeast-trending lineaments, such as the Cove Fort and Paragonah lineaments, were reactivated as right-lateral strike-slip faults. They also affected the extent of the Marysvale volcanic field.

Picha, F.; Gibson, R.I.

1985-05-01

19

Relations between hinterland and foreland shortening: Sevier orogeny, central North American Cordillera  

NASA Astrophysics Data System (ADS)

The tectonic relations between foreland and hinterland deformation in noncollisional orogens are critical to understanding the overall development of orogens. The classic central Cordilleran foreland fold-and-thrust belt in the United States (Late Jurassic to early Tertiary Sevier belt) and the more internal zones to the west (central Nevada thrust belt) provide data critical to understanding the development of internal and external parts of orogens. The Garden Valley thrust system, part of the central Nevada thrust belt, crops out in south-central Nevada within a region generally considered to be the hinterland of the Jurassic to Eocene Sevier thrust belt. The thrust system consists of at least four principal thrust plates composed of strata as young as Pennsylvanian in age that are unconformably overlain by rocks as old as Oligocene, suggesting that contraction occurred between those times. New U/Pb dates on intrusions that postdate contraction, combined with new paleomagnetic data showing significant tilting of one area prior to intrusion, suggest that regionally these thrusts were active before ˜85-100 Ma. The thrust faults are characterized by long, relatively steeply dipping ramps and associated folds that are broad and open to close, upright and overturned. Although now fragmented by Cenozoic crustal extension, individual thrusts can be correlated from range to range for tens to hundreds of kilometers along strike. We correlate the structurally lowest thrust of the Garden Valley thrust system, the Golden Gate-Mount Irish thrust, southward with the Gass Peak thrust of southern Nevada. This correlation carries the following regional implications. At least some of the slip across Jurassic to mid-Cretaceous foreland thrusts in southern Nevada continues northward along the central Nevada thrust belt rather than northeastward into Utah. This continuation is consistent with age relations, which indicate that thrusts in the type Sevier belt in central Utah are synchronous with or younger than the youngest thrusts in southern Nevada. This in turn implies that geometrically similar Sevier belt thrusts in Utah must die out southward before they reach Nevada, that slip along the southern Nevada thrusts is partitioned between central Nevada and Utah thrusts, or that the Utah thrusts persist into southeastern Nevada but are located east of the longitude of the central Nevada thrust belt. As a result of overall cratonward migration of thrusting, the central Nevada thrust belt probably formed the Cordilleran foreland fold-thrust belt early in the shortening event but later lay in the hinterland of the Sevier fold-thrust belt of Idaho-Wyoming-Utah.

Taylor, Wanda J.; Bartley, John M.; Martin, Mark W.; Geissman, John W.; Walker, J. Douglas; Armstrong, Phillip A.; Fryxell, Joan E.

2000-12-01

20

75 FR 20774 - Establishment of Class E Airspace; Mountain City, TN  

Federal Register 2010, 2011, 2012, 2013

...effective date of a direct final rule published in the Federal Register December 7, 2009 that establishes Class E airspace at Johnson County Airport, Mountain City, TN. DATES: Effective Date: 0901 UTC, April 21, 2010. FOR FURTHER INFORMATION...

2010-04-21

21

The Mormon Peak and Tule Springs Detachments of Southern Nevada and Their Role in Interpreting the Subsurface Structure of the Sevier Desert Basin  

Microsoft Academic Search

The eastern Sevier front is commonly overprinted by west-dipping normal faults that have been active through much of Cenozoic time. Although examples of disparate age and geometry abound, the Sevier Desert detachment stands out as being: (1) a very large, low-angle structure, (2) probably still active, and (3) at drillable depth, and hence is a prime target for scientific drilling.

B. Wernicke; G. J. Axen

2007-01-01

22

Preliminary Feasibility Assessment of Geologic Carbon Sequestration Potential for TVA's John Sevier and Kingston Power Plants  

SciTech Connect

This is a preliminary assessment of the potential for geologic carbon sequestration for the Tennessee Valley Authority's (TVA) John Sevier and Kingston power plants. The purpose of this assessment is to make a 'first cut' determination of whether there is sufficient potential for geologic carbon sequestration within 200 miles of the plants for TVA and Oak Ridge National Laboratory (ORNL) to proceed with a joint proposal for a larger project with a strong carbon management element. This assessment does not consider alternative technologies for carbon capture, but assumes the existence of a segregated CO{sub 2} stream suitable for sequestration.

Smith, Ellen D [ORNL; Saulsbury, Bo [ORNL

2008-03-01

23

Public Health Assessment for Evaluation of Potential Exposures to Contaminated Off-Site Groundwater from the Oak Ridge Reservation (USDOE), Oak Ridge, Anderson County, Tennessee, July 31, 2006. EPA Facility ID: TN1890090003.  

National Technical Information Service (NTIS)

In 1942, the federal government established the Oak Ridge Reservation (ORR) in Anderson and Roane Counties in Tennessee as part of the Manhattan Project to research, develop, and produce special radioactive materials for nuclear weapons. Four facilities w...

2006-01-01

24

Late Cretaceous extension in the hinterland of the Sevier thrust belt, northwestern Utah and southern Idaho  

SciTech Connect

Cover rocks of the Raft River metamorphic core complex, located in the Sevier belt hinterland, preserve a structural and metamorphic history that predates the middle Tertiary extension of the region. In the eastern Raft River Mountains, Cambrian( )-Permian rocks form two allochthons that occur in the hanging wall of the mid-Miocene Raft River detachment fault. Dramatically attenuated, metamorphosed Cambrian( )-Pennsylvanian strata of the lower allochthon yield Late Cretaceous muscovite {sup 40}Ar/{sup 39}Ar plateau ages (ca. 82-90 Ma) that are interpreted to date cooling during final stages of layer parallel extension. Devonian-Permian rocks exposed in the Black Pine Mountains underwent east-west extension ({approximately} 160%) associated with syntectonic growth of white mica along cleavage. {sup 40}Ar/{sup 39}Ar whole-rock spectra of associated slates also indicate a Late Cretaceous metamorphism. Together, field and {sup 40}Ar/{sup 39}Ar results suggest that Late Cretaceous extension occurred in the Sevier belt hinterland at the same time as shortening in the eastern foreland and at depth in the hinterland. Sufficient topography must have been present to drive upper-crustal extension in the eastern hinterland.

Wells, M.L.; Allmendinger, R.W. (Cornell Univ., Ithaca, NY (USA)); Dallmeyer, R.D. (Univ. of Georgia, Athens (USA))

1990-10-01

25

Cretaceous crustal structure and metamorphism in the hinterland of the Sevier thrust belt, western US Cordillera  

SciTech Connect

Combined structural and geochronologic studies to the west of the Sevier belt at lat 39{degree}-40{degree}N indicate that peak metamorphism of the upper 15 km of crust occurred during the Late Cretaceous (70-90 Ma), overlapping in time with the intrusion of muscovite-bearing granites and pegmatites. Metamorphism was accompanied by penetrative deformation (top-to-the-east layer-parallel shear); both were localized at the currently exposed structural levels, resulting in impressive vertical and lateral strain gradients and T{sub max} gradients of > 50 C/km. Inception of thrust faulting in the Sevier belt may have predated peak metamorphism and intrusion of granites in the hinterland by 10-15 m.y., but it continued during and ended simultaneously with the end of metamorphism at about 70 Ma. The rise of muscovite-bearing granites and hot fluids, generated by metamorphism and crustal anatexis at greater depth, provides and explanation for the observed localized metamorphism and deformation at relatively high structural levels.

Miller, E.L.; Gans, P.B. (Stanford Univ., CA (USA))

1989-01-01

26

Coal Mine Safety and Health Report of Investigation: Underground Coal Mine, Fatal Fall of Roof or Back, September 26, 2012, Double Mountain Mine, Kopper Glo Mining, LLC, Clairfield, Claiborne County, TN. ID No. 40-03365.  

National Technical Information Service (NTIS)

The Double Mountain Mine is an underground coal mine owned and operated by Quintana Energy Partners, L.P. The mine is located in Claiborne County, Tennessee and is developed in the Rich Mountain Coal Seam. The mining height ranges from 55 to 63 inches and...

2012-01-01

27

Isotopic evidence for preservation of Cordilleran lithospheric mantle during the Sevier-Laramide orogeny, western United States  

Microsoft Academic Search

It has been inferred that Sevier-Laramide flat subduction imparted a large basal shear traction force to the overriding North American plate, resulting in Laramide Rocky Mountain foreland deformation and significant thinning of Cordilleran lithosphere. Additional inferred consequences include regional refrigeration of Cordilleran crust and suppression of synorogenic extensional collapse. Nd isotopic data from Cenozoic mafic volcanic rocks indicate that normal-thickness

Richard F. Livaccari; Frank V. Perry

1993-01-01

28

9. Bridge over State Route 321 on unopened section of ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Bridge over State Route 321 on unopened section of Foothills Parkway in wear cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

29

6. LITTLE GREENBRIER SCHOOL CEMETERY AND PARKING AREA LOOKING S, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. LITTLE GREENBRIER SCHOOL CEMETERY AND PARKING AREA LOOKING S, OFF SPUR FROM METCALF BOTTOMS TO WEAR COVE. - Great Smoky Mountains National Park Roads & Bridges, Little River Road, Between Sugarlands Visitor Center & Townsend Wye, Gatlinburg, Sevier County, TN

30

18. Bridge over state route 321 on unopened section of ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

18. Bridge over state route 321 on unopened section of Foothills Parkway in wear cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

31

1. Elkmont vehicle bridge at Elkmont Campground, galvanized corrugated arch. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Elkmont vehicle bridge at Elkmont Campground, galvanized corrugated arch. - Great Smoky Mountains National Park Roads & Bridges, Elkmont Vehicle Bridge, Spanning Little River at Elkmont Campground, Gatlinburg, Sevier County, TN

32

15. Gatlinburg spur with little Pigeon River, looking toward Pigeon ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. Gatlinburg spur with little Pigeon River, looking toward Pigeon Forge. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

33

18. VIEW OF TOWNSEND WYE BRIDGE FROM LITTLE RIVER ROAD ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

18. VIEW OF TOWNSEND WYE BRIDGE FROM LITTLE RIVER ROAD LOOKING W. - Great Smoky Mountains National Park Roads & Bridges, Little River Road, Between Sugarlands Visitor Center & Townsend Wye, Gatlinburg, Sevier County, TN

34

4. Ford near the northern end of road looking NNE. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Ford near the northern end of road looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

35

1. View of Cades Cove Valley from first overlook on ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. View of Cades Cove Valley from first overlook on Rich Mountain Road looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

36

2. Switchback and General Road view looking S. Great ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Switchback and General Road view looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

37

1. Opposition sign posted on private property at the entrance ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Opposition sign posted on private property at the entrance to the road looking NW. - Great Smoky Mountains National Park Roads & Bridges, Northshore Road, 1 mile spur at Fontana Dam & Bryson City to Noland Creek, Gatlinburg, Sevier County, TN

38

10. Area of landslide on eastern section of Foothills Parkway ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Area of landslide on eastern section of Foothills Parkway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

39

Geodynamics of synconvergent extension and tectonic mode switching: Constraints from the Sevier-Laramide orogen  

NASA Astrophysics Data System (ADS)

Many orogenic belts experience alternations in shortening and extension (tectonic mode switches) during continuous plate convergence. The geodynamics of such alternations are not well understood. We present a record of Late Cretaceous to Eocene alternations of shortening and extension from the interior of the retroarc Sevier-Laramide orogen of the western United States. We integrate new Lu-Hf garnet geochronometry with revised PT paths utilizing differential thermobarometry combined with isochemical G-minimization plots, and monazite Th-Pb inclusion geochronometry to produce a well-constrained "M" shaped PTt path. Two burial events (86 and 65 Ma) are separated by ˜3 kbar of decompression. The first burial episode is Late Cretaceous, records a 2 kbar pressure increase at ˜515-550 °C and is dated by a Lu-Hf garnet isochron age of 85.5 ± 1.9 Ma (2?); the second burial episode records ˜1 kbar of pressure increase at ˜585-615 °C, and is dated by radially decreasing Th-Pb ages of monazite inclusions in garnet between ˜65 and 45 Ma. We propose a synconvergent lithospheric delamination cycle, superimposed on a dynamic orogenic wedge, as a viable mechanism. Wedge tapers may evolve from critical to subcritical (amplification), to supercritical (separation), and back to subcritical (re-equilibration) owing to elevation changes resulting from isostatic adjustments during the amplification and separation of Rayleigh-Taylor instabilities, and post-separation thermal and rheological re-equilibration. For the Sevier-Laramide hinterland, the sequence of Late Cretaceous delamination, low-angle subduction, and slab rollback/foundering during continued plate convergence explains the burial-exhumation-burial-exhumation record and the "M-shaped" PTt path.

Wells, Michael L.; Hoisch, Thomas D.; Cruz-Uribe, Alicia M.; Vervoort, Jeffrey D.

2012-02-01

40

Cyanobacterial Transposons Tn5469 and Tn5541 Represent a Novel Noncomposite Transposon Family  

PubMed Central

A noncomposite transposon, designated Tn5541, was isolated from strain Fd33 of the filamentous cyanobacterium Fremyella diplosiphon UTEX 481. Sequence analysis showed that Tn5541 is structurally and genetically very similar to Tn5469, which is also endogenous to F. diplosiphon. Both Tn5469 and Tn5541 encode homologous forms of an unusual composite transposase and a protein of unknown function. DNA hybridization analysis showed that like Tn5469, Tn5541 was not widely distributed among cyanobacterial genera. A similar analysis showed that Tn5469 and Tn5541 were equally limited to and present as multiple genomic copies in three of six distinct strains comprising the Tolypothrix 1 cluster of heterocyst-forming filamentous cyanobacteria. These and other distinguishing features suggest that Tn5469 and Tn5541 represent a novel noncomposite transposon family.

Schaefer, Michael R.; Kahn, Katherine

1998-01-01

41

Transponson Tn916 Mutagenesis in 'Bacillus anthracis',  

National Technical Information Service (NTIS)

Mutagenesis of Bacillus anthracis by the streptococcal tetracycline resistance transponson, Tn916, is described. Tn916 was transferred from Streptococcus faecalis strain DS16C1 to B. anthracis VNR-1 by conjugation in a standard filter mating procedure. Te...

B. E. Ivins S. L. Welkos G. B. Knudson D. J. LeBlanc

1987-01-01

42

Thrust controlled sedimentation patterns of the earliest Cretaceous nonmarine sequence of the Montana-Idaho-Wyoming Sevier foreland basin  

Microsoft Academic Search

Recent research on the Upper Jurassic\\/Lower Cretaceous nonmarine deposits of the central Rocky Mountain Foreland indicate that the sedimentary-tectonic model developed for Laramide intermontane basins is valid for the Sevier foreland basin as well. Episodes of thin-skinned thrusting and tectonic stacking caused asymmetric subsidence of the foreland and the localization of its structural axis adjacent to the fold-thrust belt. A

E. P. Kvale; R. A. Beck

1985-01-01

43

Role of crustal thickening and extensional collapse in the tectonic evolution of the Sevier-Laramide orogeny, western United States  

SciTech Connect

The effects of crustal thickening and extensional collapse of continental crust, combined with variations in plate-convergence forces, provide a coherent explanation for evolution of the Cretaceous-early Tertiary Sevier-Laramide orogeny. Crustal thickening of the Sevier-Laramide hinterland region was triggered by the combination of compressive plate-convergence forces and subduction-induced conductive heating of the crust. Eastward progradation of the locus of Sevier-Laramide deformation through time reflects the progressive widening of the region of crustal thickening. The region of maximum crustal thickening migrated outward until about 80 to 75 Ma, when it encountered excessively strong lithosphere of the Colorado Plateau. The locus of deformation was then transmitted laterally across both a hinterland that had attained maximum crustal thickness and the rigid Colorado Plateau, into the Laramide Rocky Mountain foreland. An episode of vigorous extensional collapse of orogenically thickened crust affected the southern Cordillera between about 75 and 35 Ma. Early Tertiary waning of plate-convergence rates and continued subduction-induced conductive heating of thickened crust allowed extensional collapse along the southern Cordillera region to drive the Colorado Plateau block northward. The result was the late Laramide phase of Rocky Mountain foreland deformation in the central and southern Laramide Rocky Mountain region continued through Eocene time, rather than ceasing at 56 Ma, as it did in the northern Cordillera.

Livaccari, R.F. (Univ. of New Mexico, Albuquerque (United States))

1991-11-01

44

Influence of Late Cretaceous magmatism on the Sevier orogenic wedge, western Montana  

NASA Astrophysics Data System (ADS)

Late Cretaceous large-volume pluton emplacement and accompanying volcanism within the evolving western Montana thrust wedge may have played important roles in determining the geometric and kinematic development of the thrust wedge, thereby influencing patterns of sediment dispersal and subsidence in the adjacent foreland basin. Intrusion of the Upper Cretaceous Boulder batholith and coeval eruption of the Elkhorn Mountains Volcanics were focused mainly at the trailing margin of the Lombard-Eldorado allochthon. The resultant thick (16 17 km) igneous culmination evolved over a relatively short interval (80 70 Ma), thickening the orogenic wedge to the point of supercritical taper and facilitating continued motion along the Lombard-Eldorado thrust system, thrust imbrication at the wedge toe, and forelandward translation of the Helena salient. Delivery of eroded volcaniclastic detritus from the thickened thrust wedge and accelerated basin subsidence due to thrust loading resulted in accumulation of a thick (4 5 km) sequence of Campanian-Maastrichtian volcaniclastic strata in the foreland basin (Livingston Group). The structural and sedimentological effects of this structural-magmatic culmination are similar to those of basement-cored culminations elsewhere in the Sevier orogen and to the Neogene central Andean orogenic wedge.

Lageson, David R.; Schmitt, James G.; Horton, Brian K.; Kalakay, Thomas J.; Burton, Bradford R.

2001-08-01

45

78 FR 58489 - Proposed Amendment of Class E Airspace; McMinnville, TN  

Federal Register 2010, 2011, 2012, 2013

This action proposes to amend Class E Airspace at McMinnville, TN, as the Warri Non-Directional Beacon (NDB) has been decommissioned and new Standard Instrument Approach Procedures have been developed at Warren County Memorial Airport. This action would enhance the safety and airspace management of Instrument Flight Rules (IFR) operations at the...

2013-09-24

46

Hydrologic evaluation and water-supply considerations for five Paiute Indian land parcels, Millard, Sevier, and Iron counties, southwestern Utah  

USGS Publications Warehouse

The hydrologic resources in and adjacent to five parcels of land held in trust for the Paiute Indian Tribe of Utah were evaluated. The land, located in southwestern Utah, is generally arid and has had only limited use for grazing. The parcels are located near the towns of Cove Fort, Joseph, Koosharem, and Kanarraville. On the basis of available geohydrologic and hydrologic data, water of suitable quality is locally available in the areas of all parcels for domestic, stock, recreation, and limited irrigation use. Developing this water for use on the parcels would potentially involve obtaining water rights, drilling wells, and constructing diversion structures. Surface water apparently is the most favorable source of supply available for the Joseph parcel, and groundwater apparently is the most favorable source of supply available for the other parcels. (USGS)

Price, Don; Stephens, D. W.; Conroy, L. S.

1989-01-01

47

Thrust belt curvature: Structural and paleomagnetic analyses in the Catalunyan Pyrenees and Sevier orogen  

NASA Astrophysics Data System (ADS)

The question of how curved geologic structures form, from arcuate faults to curvilinear orogenic belts, is one that transcends scale as well as rock properties. Many scale-independent lines of evidence suggest that material within fold-thrust belts is transported in three dimensions. Paleomagnetic analysis is the primary method for determining distribution and magnitude of vertical-axis rotations in arcuate regions. Thus paleomagnetic information is essential to deciphering the three-dimensional kinematic evolution of a curved orogen. In addition, most studies of foreland fold-and-thrust belts focus on geometries and timing relationships, but do not identify the deformations internal to the thrust-bounded rock packages. The Southern Pyrenean and Sevier orogenic systems both provide an outstanding opportunity to investigate thrust-belt curvature by integrating paleomagnetic and structural studies. This dissertation addresses the interplay between three-dimensional motions and the structures they cause. In the Pyrenees, the Oliana anticline is a foreland structure related to the South Central Salient, a major curve in the thrust belt. Paleomagnetic data from the Oliana anticline document counter clockwise rotations about a vertical axis. The average rotation is R +/- Delta R = -19.6° +/- 10.5°. Based on the stratigraphic horizons that record the rotation, as well as the structural evolution of the Oliana anticline, the age of rotation is ?35 Ma. This rotation is attributed to continued motion along the Serres Marginals thrust, causing rotation along the blind thrusts that underlie the Oliana anticline. Mode I fracture data from this region highlights the progressive development of joints in growing structures. Measurements were taken from four regions around the anticline, and from four sequential synorogenic conglomerates. A consistent NW-SW orientation for the development of joints was determined by performing sequential restorations of the Oliana anticline and incrementally analyzing joint orientations. Reanalysis of previously published data from the Wyoming salient of the western United States demonstrates the relationship between irregular fault traces and the three-dimensional motions that caused them to form. Interaction between rotation, strain and net translation is a global phenomenon and suggests that geologic shortening estimates can be improved by incorporating the effects of tectonic rotations.

Sussman, Aviva Joy

2002-04-01

48

Transposon Tn 1 intra-molecular transposition  

Microsoft Academic Search

A system for the direct selection of intra- and inter-molecular transposition events has been used to show that intra-molecular transposition of Tn1 generates deletions and inversions and requires the tnpA but not the tnpR gene product, as predicted by current models of transposition. Intra-molecular Tn1 transposition is much less limited by ‘transposition immunity’ than inter-molecular transposition, and occurs at frequencies

Richard Bishop; David Sherratt

1984-01-01

49

Structural styles of the Guess Creek fault block beneath the Great Smoky thrust sheet, Blount County, Tennessee  

SciTech Connect

A road cut along US 321 N, approximately 1 km NW of Walland, TN, exposes a previously unexposed complexly deformed section of Middle Ordovician clastic wedge [Chickamauga Group, Sevier Shale] sedimentary rocks. It provides an excellent opportunity to analyze both the lithologic assemblages and complex folding and faulting beneath the Great Smoky thrust sheet. Arkosic quartzite of the Lower Cambrian Cochran Conglomerate [Chilhowee Group], has been thrust over weaker Sevier Shale in the hanging wall of the Guess Creek fault. Regionally, the Great Smoky fault separates metamorphosed Precambrian to Lower Cambrian clastic shelf, slope, and rift facies rocks of the western Blue Ridge from Cambro-Ordovician carbonate shelf and orogenic wedge deposits of the foreland fold and thrust belt. West of the Great Smoky fault, the Guess Creek fault has been interpreted to floor duplexed Cambro-Ordovician rocks exposed in windows beneath the Great Smoky thrust sheet in the vicinity of the Great Smoky Mountains National Park. The Sevier Shale here consists of variably cleaved shale, siltstone, sandstone, and conglomerate. It exhibits a variety of fold styles throughout the exposure, ranging from predominantly noncylindrical tight folds to broad, open structures. A weak axial-planar pencil cleavage is developed in the Middle Ordovician shale and siltstone, along with a secondary cleavage that transects the axial surfaces of the folds. Minor thrust faults within the Sevier Shale appear to have formed by propagation through tightened fold hinges or bedding-parallel slip. The fold pattern observed in the roadcut appears to be partly the result of movement along a tear fault that broke both the hanging wall and footwall of the Great Smoky thrust sheet after emplacement. Slickenline orientations along minor thrust surfaces in the Cochran Conglomerate indicate eastward-directed, oblique-slip movement of the tear fault.

Carter, M.W.; Davidson, G.L.; Heller, J.A. (Univ. of Tennessee, Knoxville, TN (United States). Dept. of Geological Sciences); Hatcher, R.D. Jr. (Oak Ridge National Lab., TN (United States))

1993-03-01

50

Transposition of the kanamycin-resistance transposon Tn903  

Microsoft Academic Search

The insertion of the kanamycin-resistance transposon, Tn903, into the Escherichia coli chromosome was studied. Tn903 is similar in structure to the well known transposons Tn5 and Tn10 in that it has a unique central sequence flanked by inverted repeat sequences extending more than a thousand base pairs. However, the central region of Tn903 has enough single-frame coding capacity only for

Ry Young; Dana Smith Grillo; Ralph Isberg; Michael Syvanen

1980-01-01

51

Genetic and molecular studies of a composite chromosomal element (Tn3705) containing a Tn916-modified structure (Tn3704) in Streptococcus anginosus F22.  

PubMed

The plasmid-free Streptococcus anginosus F22 contained a conjugative element, Tn3705, encoding resistance to erythromycin (Emr) and tetracycline-minocycline (Tcr-Mnr). We mapped a chromosomal region (> 52 kb) of F22, corresponding to the internal part of Tn3705. Molecular analysis of Tn3705 revealed it to be a composite structure: it included in its central part a transposon designated Tn3704 (20.3 kb +/- 0.5 kb), which had a modified structure in comparison with that of Tn916 and on which the Emr Tcr-Mn4 markers were localized. Tn3705 inserted from F22 into the chromosome of various streptococcal transconjugants as well as that of Enterococcus faecalis transconjugants without changing its structure. In contrast, from the chromosome of an E. faecalis::Tn3705 transconjugant only Tn3704 inserted, at various sites, into another E. faecalis chromosome. Sugar fermentations occurred after the insertion of Tn3704 into the chromosome of an asaccharolytic E. faecalis strain. Transposition of only Tn3704 from the chromosome of E. faecalis::Tn3705 onto pIP964, an E. faecalis hemolysin plasmid, yielded two different pIP964 derivatives. The size of the entire element Tn3705 was estimated to be about 70.0 kb by pulsed-field electrophoresis. PMID:8171124

Clermont, D; Horaud, T

1994-01-01

52

Transformation of Mycoplasma gallisepticum with Tn916, Tn4001, and integrative plasmid vectors.  

PubMed Central

Mycoplasma gallisepticum causes respiratory disease in avian species, but little is known about its mechanism(s) of pathogenesis. These studies were undertaken in order to develop genetic systems for analysis of potential virulence factors. M. gallisepticum was transformed with plasmids containing one of the gram-positive transposons Tn916 or Tn4001, which inserted randomly into the mycoplasmal chromosome. Plasmids containing cloned chromosomal DNA were also constructed and tested for integration into regions of DNA homology derived either from chromosomal fragments or from the gentamicin resistance marker from Tn4001. These studies demonstrate that M. gallisepticum is amenable to transformation with both transposons and integrative vectors. Images

Cao, J; Kapke, P A; Minion, F C

1994-01-01

53

Layer-parallel shortening across the Sevier fold-thrust belt and Laramide foreland of Wyoming: spatial and temporal evolution of a complex geodynamic system  

NASA Astrophysics Data System (ADS)

Varying patterns of layer-parallel shortening (LPS) and vertical-axis rotations from the thin-skin Sevier fold-thrust belt to the thick-skin Laramide foreland of Wyoming are quantified from integrated structural, anisotropy of magnetic susceptibility (AMS), and paleomagnetic analyses. Within the Sevier belt, widespread early LPS was accommodated by spaced cleavage, fracture sets, minor folds, and minor faults. LPS directions are subperpendicular to structural trends of systematically curved thrust sheets of the Wyoming salient, reflecting a combination of primary dispersion and secondary rotation during thrusting. Within the Laramide foreland, limited LPS was accommodated mostly by minor faults with conjugate wedge and strike-slip geometries. LPS directions in gentler fold limbs vary from perpendicular to acute with structural trends of variably oriented, anastomosing basement-cored arches. Steep forelimbs display more complex relations, including younger fault sets that developed during evolving stress states and localized vertical-axis rotations. Although internal strain is limited, weak AMS lineations defined by kinked and rotated phyllosilicates are widely developed and consistently oriented perpendicular to measured LPS directions. Palinspastically restored LPS directions, corrected for paleomagnetically determined vertical-axis rotations, vary on average from W-E in the Sevier belt to WSW-ENE in the Laramide foreland. In detail, LPS directions display deflections related to primary sedimentary wedge geometry and basement fabrics. LPS in the Sevier belt is interpreted to partly reflect stress transmitted from the hinterland through the growing orogenic wedge and topographic stress along the front of the wedge. LPS in the Laramide foreland is interpreted to partly reflect basal traction during flat-slab subduction beneath thick cratonic lithosphere, with spatial-temporal variations in stress trajectories related to basement heterogeneities and evolving fault systems. Evidence for significant late N-S Laramide shortening, as proposed by multi-stage shortening models, is not observed.

Weil, Arlo Brandon; Yonkee, W. Adolph

2012-12-01

54

Syntectonic Fluid-Rock Interactions Involving Surficial Waters in the Sevier Thrust Belt, Tendoy Mountains, Southwest Montana  

NASA Astrophysics Data System (ADS)

Calcite veins and Mississippian carbonates from the Sevier thrust front record syntectonic meteoric fluid infiltration and hydrocarbon migration. The Tendoy and Four Eyes Canyon thrust sheets were emplaced onto the western margin of the Late Cretaceous Western Interior Seaway \\{WIS\\}. Low salinity \\{Tice = -0.6° C to +3.6° C\\} and low temperature \\{110° C +/- 10\\} fluids interacted with hanging-wall carbonates at a depth of 5km. Most veins have single or multiple generations of varying apertures, composed predominately of large euhedral crystals with some finer grained layers and protolith inclusions. Orientation analysis of mutually cross-cutting, high-angle vein sets suggest development concurrent with Four Eyes Canyon thrusting but prior to Tendoy thrusting. These vein sets are generally cut by later synfolding bed-parallel shear veins. Reactivation of both the bed-parallel and bed-perpendicular vein sets \\{strike parallel and strike perpendicular\\} in the Four Eyes Canyon thrust sheet occurred subsequent to Sevier compression, creating wide, coarse crystalline veins that often transect Sevier structures. Oxygen and Carbon isotope analyses of veins allow for reconstruction of fluid-rock interactions during thrust sheet emplacement and later reactivation. All veins and variably deformed host-rocks were microsampled and analyzed for ? 18OV-SMOW and ? 13CV-PDB. Small Tendoy veins \\{1mm-1cm wide\\} have calcite ? 18O values of +8.9 to +28.8‰ and calculated fluid \\{as H2O\\} of -8.3 to +11.6‰ \\{100° C\\}, -7.3 to +12.6‰ \\{110° C\\}, and -6.3 to +13.6‰ \\{120° C\\}. Four Eyes Canyon veins \\{1cm-3m wide\\} have calcite ? 18O values of +5.9 to +17.0‰ and calculated fluid of -11.3 to -0.2‰ \\{100° C\\}, -10.3 to +0.8‰ \\{110° C\\}, and -9.3 to +1.8‰ \\{120° C\\}. While there is significant variation in ? 18O there is relatively little systematic variation seen in ? 13C. Protolith carbonate has ? 18O values of +22.2‰ +/- 3.2; and some multi-layered veins are more depleted in ? 18O in earlier-formed generations. For three sites in the Lost River Range \\{LRR\\}, Idaho, the calculated minimum fluid ? 18O is -7.5‰ \\{+150 to +250° C\\} \\{Bebout et al., 2001; GRL\\}. Although the uncertainty of the regional temperature is large, when assuming a temperature of 110° C +/- 10 the Tendoy has a minimum calculated ? 18O H2O value of -8.3 to -6.3‰ and the Four Eyes Canyon has a minimum calculated ? 18O H2O value of -11.3 to -9.3‰ . These fluid O-isotope compositions are similar to the minimum H2O ? 18O calculated for the LRR sites - all pointing to infiltration of the thrust sheets by meteoric waters, possibly relatively nearshore meteoric waters with isotopic compositions strongly influenced by the nearby WIS. Surficial fluids possibly infiltrated into the thrust sheets by topographic recharge and migrated updip towards the foreland, mixing to varying degrees with more deeply roused fluids. Smaller veins and longer travel times and distances favored more extensive fluid-rock interaction and thus more rock-controlled fluid compositions. Microfractures in veins healed by hydrocarbons indicate that hydrocarbons migrated with freshwater fluids. Calcite veins record a dynamic history of fluid pathways and fluid flow as permeability evolved during thrust emplacement.

Johnson, A. C.; Anastasio, D. J.; Bebout, G. E.

2002-05-01

55

Detection of Tn917-Like Sequences within a Tn916-like Conjugative Transposon (Tn3872) in Erythromycin-Resistant Isolates of Streptococcus pneumoniae  

PubMed Central

A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization. Like Tn1545, Tn917 also encodes an ermAM gene but does not mediate resistance to other antimicrobial agents. Furthermore, nucleotide sequence analyses of the DNAs flanking three of the Tn917-like elements revealed that they were inserted into orf9 of a Tn916-like element in a composite transposon-like structure (Tn3872). Other MLS-resistant strains appeared to contain Tn1545-like elements that had suffered a deletion of sequences including the aphA-3 sequences responsible for kanamycin resistance. Thus, the MLS resistance phenotype in pneumococci appears to be mediated by the ermAM present on a much wider variety of genetic elements than was previously appreciated.

McDougal, Linda K.; Tenover, Fred C.; Lee, Linda N.; Rasheed, J. Kamile; Patterson, Jan E.; Jorgensen, James H.; LeBlanc, Donald J.

1998-01-01

56

77 FR 61653 - Notice of Intent To Rule on Request To Release Airport Property at the Smyrna-Rutherford County...  

Federal Register 2010, 2011, 2012, 2013

...Notice of Intent To Rule on Request To Release Airport Property at the Smyrna-Rutherford County Airport, Smyrna, TN AGENCY: Federal Aviation Administration...of land at the Smyrna-Rutherford County Airport, Smyrna, Tennessee. This property,...

2012-10-10

57

Unconstrained bacterial promiscuity: the Tn 916–Tn 1545 family of conjugative transposons  

Microsoft Academic Search

Conjugative transposons are highly ubiquitous elements found throughout the bacterial world. Members of the Tn916–Tn1545 family carry the widely disseminated tetracycline-resistance determinant Tet M, as well as additional resistance genes. They have been found naturally in, or been introduced into, over 50 different species and 24 genera of bacteria. Recent investigations have led to insights into the molecular basis of

D. B. Clewell; S. E. Flannagan; D. D. Jaworski

1995-01-01

58

BAM Media M150: Trypticase Novobiocin (TN) Broth  

Center for Food Safety and Applied Nutrition (CFSAN)

... BAM Media M150: Trypticase Novobiocin (TN) Broth. January 2001. Bacteriological Analytical Manual. M150 Trypticase Novobiocin (TN) Broth. ... More results from www.fda.gov/food/foodscienceresearch/laboratorymethods

59

Mercury(II)-resistance transposons Tn502 and Tn512, from Pseudomonas clinical strains, are structurally different members of the Tn5053 family.  

PubMed

The mercury(II)-resistance transposons Tn502 and Tn512 were sequenced and shown to be members of the Tn5053 family. They are currently the sole representatives from the clinical setting and were obtained from geographically disparate Pseudomonas aeruginosa strains. The family is comprised of six novel transposons that display genetic and structural variability that has arisen in different ways. The mer and tni arms of the transposons can be differently combined, suggesting that chimeric interchanges have occurred, possibly mediated by the TniR resolvase. The mer modules within the mer arms are remnants of Tn21/Tn501-like transposons that inserted into a tni-containing ancestral transposon. The mer modules themselves are polymorphic and that of Tn502 is a new type. It includes the putative urf2M gene, the 3'-end of which expresses a protein and hence is a bone fide gene (tniM). Homologues of tniM occur beyond the Tn5053 family and include Tn21 tnpM. Other studies have implicated tniM and tnpM in transposition. Based on sequence and compositional differences, members of the environmentally widespread Tn5053 family constitute a different lineage from the related and clinically successful intI-containing Tn402 family. PMID:20800080

Petrovski, Steve; Blackmore, Dale W; Jackson, Kerry L; Stanisich, Vilma A

2010-08-25

60

ATP-dependent specific binding of Tn3 transposase to Tn3 inverted repeats  

NASA Astrophysics Data System (ADS)

Transposons are discrete segments of DNA which are capable of moving from one site in a genome to many different sites1,2. Tn3 is a prokaryotic transposon which is 4,957 base pairs (bp) long and encodes a transposase protein which is essential for transposition3-7. We report here a simple method for purifying Tn3 transposase and demonstrate that the transposase protein binds specifically to the ends of the Tn3 transposon in an ATP-dependent manner. The transposase protein binds to linear double-stranded DNA both nonspecifically and specifically; the nonspecific DNA binding activity is sensitive to challenge with heparin. Site-specific DNA binding to the ends (inverted repeats) of Tn3 is observed only when binding is performed in the presence of ATP; this ATP-dependent site-specific DNA binding activity is resistant to heparin challenge. Our results indicate that ATP qualitatively alters the DNA binding activity of the transposase protein so that the protein is able to bind specifically to the ends of the Tn3 transposon.

Wishart, W. L.; Broach, J. R.; Ohtsubo, E.

1985-04-01

61

Nucleotide sequences required for Tn3 transposition immunity.  

PubMed Central

The Tn3 transposon inserts at a reduced frequency into a plasmid already containing a copy of Tn3, a phenomenon known as transposition immunity. The cis-acting site on Tn3 responsible for immunity was mapped by deletions from each side to be within the terminal 38-base-pair sequence that is inversely repeated at the ends of Tn3. Two palindromic sequences are present in the essential part of this region. Some deletions conferred only partial immunity, and others conferred negative immunity. Multiple copies of partially immune ends conferred additional immunity. No other part of Tn3 was necessary for immunity.

Kans, J A; Casadaban, M J

1989-01-01

62

Streptococcus pneumoniae Transposon Tn1545/Tn6003 Changes to Tn6002 Due to Spontaneous Excision in Circular Form of the erm(B)- and aphA3-Containing Macrolide-Aminoglycoside-Streptothricin (MAS) Element  

PubMed Central

The macrolide-aminoglycoside-streptothricin (MAS) element, an ?4.2-kb insertion containing erm(B) and aphA3 resistance determinants, distinguishes Streptococcus pneumoniae transposon Tn1545/Tn6003 from Tn6002. Here, it is shown to be an unstable genetic element that, although it lacks recombinase genes, can exploit long, erm(B)-containing direct repeats acting as att sites for spontaneous excision that may result in loss. Consequent to excision, which is RecA independent, Tn1545/Tn6003 changes to Tn6002. In pneumococcal populations harboring Tn1545/Tn6003, the latter appears to coexist with Tn6002.

Palmieri, Claudio; Mingoia, Marina; Massidda, Orietta; Giovanetti, Eleonora

2012-01-01

63

Characterization of the gentamicin resistance transposon Tn5281 from Enterococcus faecalis and comparison to staphylococcal transposons Tn4001 and Tn4031.  

PubMed Central

In Enterococcus faecalis, the genetic determinant encoding gentamicin resistance (Gmr) on the conjugative plasmid pBEM10 previously has been shown to be on a mobile element. In the current study, this element, termed Tn5281, was shown to relocate in the absence of homologous recombination in E. faecalis UV202. On the basis of restriction endonuclease analysis and DNA-DNA hybridization studies, Tn5281 was shown to be similar, if not identical, to the Gmr transposons Tn4001 found in Australian isolates of Staphylococcus aureus and Tn4031 found in U.S. isolates of Staphylococcus epidermidis, since all three of these transposons have symmetrically located HindIII (2.5 kb apart), ClaI (slightly more than 2.5 kb apart), and HaeIII (3.9 kb apart) sites. Restriction endonuclease digestion patterns of Tn5281 generated with HincII, ScaI, and AluI were also consistent with Tn4001 and Tn4031. By using a probe specific for the external portion of the terminal inverted repeat of Tn4031, it was determined that each terminus of Tn5281 contained a 0.35-kb HaeIII fragment and a 0.7-kb HindIII-HaeIII fragment. The sizes of these fragments are identical to those found in the staphylococcal transposons, which is a further indication that inverted repeats like IS256 are present in Tn5281. A 1-kb HaeIII fragment in pBEM10 also hybridized with this probe, which indicates that Tn5281 in pBEM10 contains a double copy of the inverted repeat at one end. Images

Hodel-Christian, S L; Murray, B E

1991-01-01

64

Site-specific Tn7 transposition into the human genome  

Microsoft Academic Search

The bacterial transposon, Tn7, inserts into a single site in the Escherichia coli chromosome termed attTn7 via the sequence-specific DNA binding of the target selector protein, TnsD. The target DNA sequence required for Tn7 transposition is located within the C-terminus of the glucosamine synthetase (glmS) gene, which is an essential, highly conserved gene found ubiquitously from bacteria to humans. Here,

Prasad N. Kuduvalli; Rupak Mitra; Nancy L. Craig

2005-01-01

65

Functional Characterization of Tn4401, a Tn3-Based Transposon Involved in blaKPC Gene Mobilization ?  

PubMed Central

The carbapenemase gene blaKPC, which is rapidly spreading worldwide, is located on a Tn3-based transposon, Tn4401. In a transposition-conjugation assay, Tn4401 was able to mobilize blaKPC-2 gene at a frequency of 4.4 × 10?6/recipient cell. A 5-bp target site duplication was evidenced upon each insertion without target site specificity. This study demonstrated that Tn4401 is an active transposon capable of mobilizing blaKPC genes at high frequency.

Cuzon, Gaelle; Naas, Thierry; Nordmann, Patrice

2011-01-01

66

48 CFR 1201.301-72 - TAC or TN numbering.  

Code of Federal Regulations, 2010 CFR

...Regulations System 5 2009-10-01 2009-10-01 false TAC or TN numbering. 1201.301-72 Section 1201.301-72...REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered...

2009-10-01

67

48 CFR 1201.301-72 - TAC or TN numbering.  

Code of Federal Regulations, 2012 CFR

...Regulations System 5 2012-10-01 2012-10-01 false TAC or TN numbering. 1201.301-72 Section 1201.301-72...REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered...

2012-10-01

68

48 CFR 1201.301-72 - TAC or TN numbering.  

Code of Federal Regulations, 2011 CFR

...Regulations System 5 2011-10-01 2011-10-01 false TAC or TN numbering. 1201.301-72 Section 1201.301-72...REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered...

2011-10-01

69

48 CFR 1201.301-72 - TAC or TN numbering.  

Code of Federal Regulations, 2010 CFR

...Regulations System 5 2010-10-01 2010-10-01 false TAC or TN numbering. 1201.301-72 Section 1201.301-72...REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered...

2010-10-01

70

76 FR 55232 - Establishment of Class E Airspace; Copperhill, TN  

Federal Register 2010, 2011, 2012, 2013

...Approach Procedures developed for Martin Campbell Field Airport. This...establishes controlled airspace at Martin Campbell Field Airport, Copperhill...DESIGNATION OF CLASS A, B, C, D AND E AIRSPACE AREAS; AIR TRAFFIC...TN E5 Copperhill, TN [New] Martin Campbell Field Airport,...

2011-09-07

71

78 FR 38555 - Amendment of Class E Airspace; Selmer, TN  

Federal Register 2010, 2011, 2012, 2013

...Instrument Flight Rules (IFR) operations at Robert Sibley Airport. This enhances the safety...NPRM) to amend Class E airspace at Robert Sibley Airport, Selmer, TN. (78 FR...surface within a 6.7-mile radius of Robert Sibley Airport, Selmer, TN....

2013-06-27

72

The catalytic residues of Tn3 resolvase  

PubMed Central

To characterize the residues that participate in the catalysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase, we mutated conserved polar or charged residues in the catalytic domain of an activated resolvase variant. We analysed the effects of mutations at 14 residues on proficiency in binding to the recombination site (‘site I’), formation of a synaptic complex between two site Is, DNA cleavage and recombination. Mutations of Y6, R8, S10, D36, R68 and R71 resulted in greatly reduced cleavage and recombination activity, suggesting crucial roles of these six residues in catalysis, whereas mutations of the other residues had less dramatic effects. No mutations strongly inhibited binding of resolvase to site I, but several caused conspicuous changes in the yield or stability of the synapse of two site Is observed by non-denaturing gel electrophoresis. The involvement of some residues in both synapsis and catalysis suggests that they contribute to a regulatory mechanism, in which engagement of catalytic residues with the substrate is coupled to correct assembly of the synapse.

Olorunniji, Femi J.; Stark, W. Marshall

2009-01-01

73

19. Photographer unknown, circa 1950 'THE LOOP OVER' BRIDGE WAS ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. Photographer unknown, circa 1950 'THE LOOP OVER' BRIDGE WAS CONSTRUCTED ON NEWFOUND GAP ROAD TO REPLACE A SERIES OF DANGEROUS SWITCHBACKS. THIS ENGINEERING FEATURE QUICKLY BECAME A POPULAR TOURIST ATTRACTION. - Great Smoky Mountains National Park Roads & Bridges, Gatlinburg, Sevier County, TN

74

Kinematic history of the Meade Thrust based on provenance of the Bechler conglomerate at Red Mountain, Idaho, Sevier Thrust Belt  

NASA Astrophysics Data System (ADS)

With up to ˜45 km of slip, the Meade thrust fault in southeastern Idaho is one of the major thrusts in the Sevier thrust belt, yet its age of displacement has remained enigmatic because of the lack of a derivative synorogenic deposit. We propose that the synorogenic conglomerate produced by initial Meade thrusting crops out on Red Mountain, in southeastern Idaho, ˜1.5 km east of the present trace of the Meade thrust. This conglomerate historically has been considered part of the Ephraim Formation of the Lower Cretaceous Gannett Group, but we suggest that it is a conglomeratic facies of the Bechler Formation localized to the area of Red Mountain. Regional stratigraphic considerations and paleontological dates from underlying and overlying strata indicate that the Bechler conglomerate facies (BCF) is Aptian in age. The BCF is 850 m thick and consists of pebble- to boulder-conglomerate, sandstone, and mudrock deposited by fluvial and mass flow processes on medial to distal parts of an alluvial fan. Paleocurrent data indicate an eastward, fan-shaped dispersal pattern. The BCF contains clasts of micritic limestone, chert-pebble conglomerate, and cherty litharenite that were derived from the Ephraim Formation. In addition, the conglomerate contains abundant clasts of Ordovician, Carboniferous, and lower Mesozoic rocks that crop out on both the Meade thrust sheet and the Paris thrust sheet ˜25-30 km to the west. The base of the BCF is marked by a local 27° angular unconformity on top of the Ephraim Formation. The BCF contains intraformational, progressively rotated angular unconformities, internal growth folds, and minor dip discontinuities that were produced by simultaneous folding and sediment accumulation on the proximal footwall of the Meade thrust. Provenance modeling indicates that the BCF was derived from Mesozoic strata that were exposed along the frontal part of the Meade thrust sheet and from Paleozoic strata that were coevally exposed in the hindward located Paris thrust sheet. Topography on the Paris sheet was rejuvenated as it was carried passively over a major ramp in the Meade thrust. Provenance and structural data are combined to produce an incremental, bulk-rock retrodeformation of initial Meade displacement. Clasts of Jurassic Twin Creek Limestone with pressure-solution cleavage in the lower part of the BCF indicate that an episode of layer-parallel shortening occurred in the Meade hanging wall prior to Meade displacement; this may have been related to emplacement of the Paris thrust sheet. The BCF recorded ˜6 km of initial Meade-related shortening by thrust slip and fault-propagation folding during Aptian time. Illite crystallinity, systematic fracture sets in the Ephraim Formation and BCF, finite strain in Meade footwall and hanging wall rocks, and thermal models by previous workers indicate that the Meade thrust sheet ultimately overrode the BCF and other footwall rocks, probably during Albian-Cenomanian time. The BCF and the Meade thrust were folded during slip on thrust faults related to the northern Crawford thrust during Coniacian time. A revised interpretation of Gannett Group provenance throughout the thrust belt suggests that the Ephraim Formation was derived from both the Paris thrust sheet and an older, hindward located thrust sheet. The Bechler was derived from the Meade and Paris sheets.

Decelles, P. G.; Pile, H. T.; Coogan, J. C.

1993-12-01

75

77 FR 13195 - Amendment of Class E Airspace; Springfield, TN  

Federal Register 2010, 2011, 2012, 2013

...geographic coordinates of the Springfield Robertson County Airport. DATES: Effective 0901...geographic coordinates for Springfield Robertson County Airport needed to be adjusted...geographic coordinates of the Springfield Robertson County Airport to be in concert...

2012-03-06

76

Tn7 elements: Engendering diversity from chromosomes to episomes  

PubMed Central

The bacterial transposon Tn7 maintains two distinct lifestyles, one in horizontally transferred DNA and the other in bacterial chromosomes. Access to these two DNA pools is mediated by two separate target selection pathways. The proteins involved in these pathways have evolved to specifically activate transposition into their cognate target-sites using entirely different recognition mechanisms, but the same core transposition machinery. In this review we discuss how the molecular mechanisms of Tn7-like elements contribute to their diversification and how they affect the evolution of their host genomes. The analysis of over 50 Tn7-like elements provides insight into the evolution of Tn7 and Tn7 relatives. In addition to the genes required for transposition, Tn7-like elements transport a wide variety of genes that contribute to the success of diverse organisms. We propose that by decisively moving between mobile and stationary DNA pools, Tn7-like elements accumulate a broad range of genetic material, providing a selective advantage for diverse host bacteria.

Parks, Adam R.; Peters, Joseph E.

2008-01-01

77

Site-specific Tn7 transposition into the human genome  

PubMed Central

The bacterial transposon, Tn7, inserts into a single site in the Escherichia coli chromosome termed attTn7 via the sequence-specific DNA binding of the target selector protein, TnsD. The target DNA sequence required for Tn7 transposition is located within the C-terminus of the glucosamine synthetase (glmS) gene, which is an essential, highly conserved gene found ubiquitously from bacteria to humans. Here, we show that Tn7 can transpose in vitro adjacent to two potential targets in the human genome: the gfpt-1 and gfpt-2 sequences, the human analogs of glmS. The frequency of transposition adjacent to the human gfpt-1 target is comparable with the E.coli glmS target; the human gfpt-2 target shows reduced transposition. The binding of TnsD to these sequences mirrors the transposition activity. In contrast to the human gfpt sequences, Tn7 does not transpose adjacent to the gfa-1 sequence, the glmS analog in Saccharomyces cerevisiae. We also report that a nucleosome core particle assembled on the human gfpt-1 sequence reduces Tn7 transposition by likely impairing the accessibility of target DNA to the Tns proteins. We discuss the implications of these findings for the potential use of Tn7 as a site-specific DNA delivery agent for gene therapy.

Kuduvalli, Prasad N.; Mitra, Rupak; Craig, Nancy L.

2005-01-01

78

Insertion sites and the terminal nucleotide sequences of the Tn4 transposon.  

PubMed Central

The nucleotide sequences at the ends of the Tn4 transposon (mercury spectinomycin and sulfonamide resistance) have been determined. They are inverted repeated sequences of 38 nucleotides with three mismatched base pairs. These sequences are strongly homologous with the terminal sequences of Tn501 (mercury resistance) but less so with those of Tn3 (ampicillin resistance). The Tn4 transposon generates pentanucleotide members (Tn3, Tn1000, Tn501, Tn551, IS2) with the exception of Tn1721 and bacteriophage Mu. Among the three Tn4 insertion sites examined here, two of them occurred near a nonanucleotide sequence in perfect homology with part of the terminal inverted-repeat sequence of Tn4 and the third insertion occurred near a sequence of partial homology to one end of Tn4. All three insertions were in the same orientation such that IRb is proximal to its homologous sequence on the recipient DNA. Images

Hyde, D R; Tu, C P

1982-01-01

79

Tn502 and Tn512 are res site hunters that provide evidence of resolvase-independent transposition to random sites.  

PubMed

In this study, we report on the transposition behavior of the mercury(II) resistance transposons Tn502 and Tn512, which are members of the Tn5053 family. These transposons exhibit targeted and oriented insertion in the par region of plasmid RP1, since par-encoded components, namely, the ParA resolvase and its cognate res region, are essential for such transposition. Tn502 and, under some circumstances, Tn512 can transpose when par is absent, providing evidence for an alternative, par-independent pathway of transposition. We show that the alternative pathway proceeds by a two-step replicative process involving random target selection and orientation of insertion, leading to the formation of cointegrates as the predominant product of the first stage of transposition. Cointegrates remain unresolved because the transposon-encoded (TniR) recombination system is relatively inefficient, as is the host-encoded (RecA) system. In the presence of the res-ParA recombination system, TniR-mediated (and RecA-mediated) cointegrate resolution is highly efficient, enabling resolution both of cointegrates involving functional transposons (Tn502 and Tn512) and of defective elements (In0 and In2). These findings implicate the target-encoded accessory functions in the second stage of transposition as well as in the first. We also show that the par-independent pathway enables the formation of deletions in the target molecule. PMID:20118251

Petrovski, Steve; Stanisich, Vilma A

2010-01-29

80

Tn502 and Tn512 Are res Site Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites ?  

PubMed Central

In this study, we report on the transposition behavior of the mercury(II) resistance transposons Tn502 and Tn512, which are members of the Tn5053 family. These transposons exhibit targeted and oriented insertion in the par region of plasmid RP1, since par-encoded components, namely, the ParA resolvase and its cognate res region, are essential for such transposition. Tn502 and, under some circumstances, Tn512 can transpose when par is absent, providing evidence for an alternative, par-independent pathway of transposition. We show that the alternative pathway proceeds by a two-step replicative process involving random target selection and orientation of insertion, leading to the formation of cointegrates as the predominant product of the first stage of transposition. Cointegrates remain unresolved because the transposon-encoded (TniR) recombination system is relatively inefficient, as is the host-encoded (RecA) system. In the presence of the res-ParA recombination system, TniR-mediated (and RecA-mediated) cointegrate resolution is highly efficient, enabling resolution both of cointegrates involving functional transposons (Tn502 and Tn512) and of defective elements (In0 and In2). These findings implicate the target-encoded accessory functions in the second stage of transposition as well as in the first. We also show that the par-independent pathway enables the formation of deletions in the target molecule.

Petrovski, Steve; Stanisich, Vilma A.

2010-01-01

81

76 FR 33656 - Television Broadcasting Services; Nashville, TN  

Federal Register 2010, 2011, 2012, 2013

...Part 73 [MB Docket No. 11-29; RM-11622, DA 11-949] Television Broadcasting Services; Nashville, TN AGENCY: Federal Communications...801(a)(1)(A). List of Subjects in 47 CFR Part 73 Television. Federal Communications Commission. Barbara A....

2011-06-09

82

76 FR 14855 - Television Broadcasting Services; Nashville, TN  

Federal Register 2010, 2011, 2012, 2013

...Docket No. 11-29, RM-11622; DA 11-335] Television Broadcasting Services; Nashville, TN AGENCY...and 1.420. List of Subjects in 47 CFR Part 73 Television, Television broadcasting. Federal Communications...

2011-03-18

83

Degradation of TN-C component of troponin by trypsin.  

PubMed

The peptide pattern obtained during degradation of TN-C component of troponin by trypsin markedly depends on the concentration of calcium ions. In the presence of calcium higher than 0.1 mM a peptide with a mass of about 16 000 daltons is formed, followed by its splitting into two peptides (8500 and 7500 daltons), which accumulate during further digestion. When calcium is sequestred by EGTA the degradation of TN-C is much faster. The first products of digestion are the 11 000 and 7500 dalton peptides. The former one is degraded to 9700 dalton peptide and, subsequently, both peptides are split to small fragments. In the presence of calcium only three -NH2 groups per molecule of TN-C appear during digestion, whereas in its absence almost all peptide bonds available for trypsin are cleaved. The fragments of TN-C obtained in the presence of calcium are still able to interact with TN-I component as judged by disc-electrophoresis performed in the presence of calcium. During digestion in the presence of calcium only a slight decrease of tyrosyl intrinsic fluorescence occurs and the further decrease after removal of calcium bound to TN-C is fully reversible. Similarly, the content of alpha-helix decreases only slowly during digestion in the presence of calcium. All the results suggest that calcium stabilizes the structure of TN-C molecule, so that trypsin in the presence of calcium releases one small peptide from N-terminal not involved in the first "EF-hand" and the other from the region between pairs 1-2 and 3-4 of "EF-hands" in the nomenclature of Kretsinger (see Kretsinger and Barry [16]). The obtained fragments, containing almost exclusively pairs of "EF-hands", restore most of the properties of original TN-C molecule. PMID:836870

Drabikowski, W; Grabarek, Z; Barylko, B

1977-01-25

84

Characterization of transposon Tn5469 from the cyanobacterium Fremyella diplosiphon.  

PubMed Central

A transposon, designated Tn5469, was isolated from mutant strain FdR1 of the filamentous cyanobacterium Fremyella diplosiphon following its insertion into the rcaC gene. Tn5469 is a 4,904-bp noncomposite transposon with 25-bp near-perfect terminal inverted repeats and has three tandemly arranged, slightly overlapping potential open reading frames (ORFs) encoding proteins of 104.6 kDa (909 residues), 42.5 kDa (375 residues), and 31.9 kDa (272 residues). Insertion of Tn5469 into the rcaC gene in strain FdR1 generated a duplicate 5-bp target sequence. On the basis of amino acid sequence identifies, the largest ORF, designated tnpA, is predicted to encode a composite transposase protein. A 230-residue domain near the amino terminus of the TnpA protein has 15.4% amino acid sequence identity with a corresponding domain for the putative transposase encoded by Lactococcus lactis insertion sequence S1 (ISS1). In addition, the sequence for the carboxyl-terminal 600 residues of the TnpA protein is 20.0% identical to that for the TniA transposase encoded by Tn5090 on Klebsiella aerogenes plasmid R751. The TnpA and TniA proteins contain the D,D(35)E motif characteristic of a recently defined superfamily consisting of bacterial transposases and integrase proteins of eukaryotic retroelements and retrotransposons. The two remaining ORFs on Tn5469 encode proteins of unknown function. Southern blot analysis showed that wild-type F. diplosiphon harbors five genomic copies of Tn5469. In comparison, mutant strain FdR1 harbors an extra genomic copy of Tn5469 which was localized to the inactivated rcaC gene. Among five morphologically distinct cyanobacterial strains examined, none was found to contain genomic sequences homologous to Tn5469.

Kahn, K; Schaefer, M R

1995-01-01

85

Combining paleomagnetism and structural geology to better understand complex orogenic systems: development of a robust kinematic model for Sevier fold-thrust belt and Laramide foreland of Wyoming, U.S.A.  

NASA Astrophysics Data System (ADS)

Varying patterns of layer-parallel shortening (LPS) and vertical-axis rotations from the thin-skin Sevier fold-thrust belt to the thick-skin Laramide foreland of Wyoming are quantified from integrated structural, anisotropy of magnetic susceptibility (AMS), and paleomagnetic analyses. Within the Sevier belt, widespread early LPS was accommodated by spaced cleavage, fracture sets, minor folds, and minor faults. LPS directions are subperpendicular to structural trends of systematically curved thrust sheets of the Wyoming salient, reflecting a combination of primary dispersion and secondary rotation during thrusting. Within the Laramide foreland, limited LPS was accommodated mostly by minor faults with conjugate wedge and strike-slip geometries. LPS directions in gentler fold limbs vary from perpendicular to acute with structural trends of variably oriented, anastomosing basement-cored arches. Steep forelimbs display more complex relations, including younger fault sets that developed during evolving stress states and localized vertical-axis rotations. Although internal strain is limited, weak AMS lineations defined by kinked and rotated phyllosilicates are widely developed and consistently oriented perpendicular to measured LPS directions. Palinspastically restored LPS directions, corrected for paleomagnetically determined vertical-axis rotations, vary on average from W-E in the Sevier belt to WSW-ENE in the Laramide foreland. In detail, LPS directions display deflections related to primary sedimentary wedge geometry and basement fabrics. LPS in the Sevier belt is interpreted to partly reflect stress transmitted from the hinterland through the growing orogenic wedge and topographic stress along the front of the wedge. LPS in the Laramide foreland is interpreted to partly reflect basal traction during flat-slab subduction beneath thick cratonic lithosphere, with spatial-temporal variations in stress trajectories related to basement heterogeneities and evolving fault systems. Evidence for significant late N-S Laramide shortening, as proposed by multi-stage shortening models, is not observed.

Weil, Arlo; Yonkee, Adolph

2013-04-01

86

Immune sera and monoclonal antibodies define two configurations for the sialyl Tn tumor antigen.  

PubMed

Sialyl Tn (sTn) is a mucin-associated carbohydrate antigen expressed in most types of human adenocarcinoma. Defining the configuration of tumor cell surface sTn recognized by antibodies is important for understanding the basis for the cancer cell specificity of sTn-reactive mAbs, for the development of more effective mAbs, and for designing cancer vaccines against sTn. In this study, we compared the immunogenicity of synthetic single sTn disaccharide epitopes and clusters [sTn(C)] of 3 sTn epitopes covalently linked via serine to keyhole limpet hemocyanin [KLH; sTn-KLH and sTn(C)-KLH, respectively]. The cell surface sTn configurations were analyzed with the use of sera from mice immunized with these neoglycoproteins and a panel of sTn-reactive mAb. Sera from mice immunized with sTn-KLH reacted in direct and inhibition assays with sTn-human serum albumin (HSA) but only weakly with sTn(C)-HSA, whereas sera from mice immunized with sTn(C)-KLH reacted with sTn(C)-HSA but not with sTn-HSA. Both anti-sTn and anti-sTn(C) sera reacted with ovine submaxillary mucin (a natural source of sTn) and with sTn-positive human tumor cell line LS-C but not with sTn-negative LS-B cells. With regard to the sTn-reactive mAbs, B72.3 reacted exclusively with clustered sTn, whereas mAb B195.3R11 reacted preferentially with unclustered sTn. Results with mAbs TKH2, B239.1, and CC49 were less clear, although all reacted more strongly with clustered sTn than with unclustered sTn. These results suggest that sTn is recognized at the tumor cell surface in at least two quite distinct configurations, as clustered and nonclustered arrays. PMID:7614472

Zhang, S; Walberg, L A; Ogata, S; Itzkowitz, S H; Koganty, R R; Reddish, M; Gandhi, S S; Longenecker, B M; Lloyd, K O; Livingston, P O

1995-08-01

87

Magnitude, Timing, and Geometry of Extension in the Southern Sevier Desert Basin From Piercing Points, Seismic-Stratigraphic Reconstruction, and Deep well Data  

NASA Astrophysics Data System (ADS)

Palinspastic reconstruction of Mesozoic thrust sheets provides the main constraint for an estimated 47 km of Cenozoic extensional displacement along the Sevier Desert detachment (SDD) in the central Sevier Desert Basin. Hanging wall and footwall piercing points indicate that the SDD accommodated a minimum of 35 km of extensional displacement in the narrower southern part of the basin. The piercing points for the SDD are defined by the intersection of the SDD, the Canyon Range thrust (CRT), and a regional early Cenozoic erosion surface (ES). The hanging wall piercing point lies immediately northeast of the Cricket Mountains, where the SDD-CRT- ES intersection is narrowly defined by intersecting structure maps derived from published seismic reflection data. The footwall piercing point lies in the southern foothills of the Canyon Range, where the SDD breakaway plane is well constrained by an industry seismic line that lies within 2 km of the exposed intersection of the CRT with the base of the Oligocene Oak City Formation. Timing of extension in the southern Sevier Desert basin is constrained by a kinematic reconstruction of detachment and imbricate fault displacement, footwall uplift, and supradetachment sedimentation for Oligocene, Miocene, and Plio-Pleistocene seismic sequences. The reconstruction is centered on a seismic reflection and gravity interpretation along the published Pan Canadian profiles 2 and 3 that is tied to dated intervals in six industry wells. Fault restoration indicates that Oligocene and Miocene phases of slip each accounted for about 40 percent of the total displacement. Simultaneous backstripping of the Oligocene, Miocene, and Plio-Pleistocene supradetachment sequences records hanging wall subsidence simultaneous with footwall uplift, with a footwall burial history that is consistent with published Miocene apatite and zircon fission-track ages of footwall samples. The geometric evolution of the southern SDD extensional system is consistent with its development above a broad westward-migrating "rolling hinge" zone associated with isostatic uplift of the detachment footwall. Hanging wall normal faults east of the footwall crest exhibit small post-Miocene displacement, with demonstrable Quaternary slip restricted to the crest and western limb of the uplift, most notably along the Black Rock and Clear Lake fault zones. Early abandonment of the eastern part of the detachment may explain the indistinct geomorphic and structural expression of the break-away zone at the surface. The deepest level of the southern SDD also presents a complex geometry and kinematic history. The 1996 Chevron 1-29 Black Rock Federal well through the western basin margin penetrated a normal fault that places Jurassic over lower Cambrian strata at 4650 m measured depth, well above the principal SDD seismic reflection. The fault is not correlated to any large- displacement high-angle fault at shallow levels, and may form the abandoned roof to an extensional duplex.

Coogan, J. C.; Decelles, P. G.

2007-12-01

88

Location of an ampicillin resistance transposon, Tn1701, in a group of small, nontransferring plasmids.  

PubMed Central

By restriction endonuclease cleavage mapping and electron microscopic examination of heteroduplexes, we have identified an ampicillin resistance determinant transposon, designated Tn1701, in a group of small, nontransferring plasmids which confer resistance to ampicillin (Ap), sulfonamide (Su), and streptomycin (Sm). Plasmid NTP1, which mediates Ap resistance, contains Tn1701. Recombinant plasmids NTP3 (Ap Su) and NTP4 (Ap Su Sm) contain Tn1701, indicating that they were derived by transposition of Tn1701 from NTP1 to an unrelated plasmid, NTP2 (Su Sm). The transposon Tn1701 is very similar to the known ampicillin resistance transposons Tn1, Tn2, and Tn3 in its size (3.2 x 10(6) daltons), base sequence homology observed by heteroduplex formation, restriction endonuclease cleavage sites, and possession of a short inverted repeat sequence at both ends. Like the other TnA elements, Tn1701 also specifies a type TEM beta-lactamase. Images

Yamada, Y; Calame, K L; Grindley, J N; Nakada, D

1979-01-01

89

In vivo Tn5-based transposon mutagenesis of Streptomycetes.  

PubMed

This paper reports the in vivo expression of the synthetic transposase gene tnp(a) from a hyperactive Tn5 tnp gene mutant in Streptomyces coelicolor. Using the synthetic tnp(a) gene adapted for Streptomyces codon usage, we showed random insertion of the transposon into the Streptomycetes genome. The insertion frequency for the hyperactive Tn5 derivative is 98% of transformed S. coelicolor cells. The random transposition has been confirmed by the recovery of ~1.1% of auxotrophs. The Tn5 insertions are stably inherited in the absence of apramycin selection. The transposon contains an apramycin resistance selection marker and an R6Kgamma origin of replication for transposon rescue. We identified the transposon insertion loci by random sequencing of 14 rescue plasmids. The majority of insertions (12 of 14) were mapped to putative open-reading frames on the S. coelicolor chromosome. These included two new regulatory genes affecting S. coelicolor growth and actinorhodin biosynthesis. PMID:19479250

Petzke, Lutz; Luzhetskyy, Andriy

2009-05-29

90

76 FR 27739 - Tennessee Disaster Number TN-00051  

Federal Register 2010, 2011, 2012, 2013

...the disaster: Primary Counties: (Physical Damage and Economic Injury Loans): Bledsoe, Cocke, Johnson, McMinn, Monroe, Rhea. Contiguous Counties: (Economic Injury Loans Only): Tennessee: Blount, Cumberland, Jefferson, Loudon, Roane,...

2011-05-12

91

Construction of an Enterococcus faecalis Tn917-Mediated-Gene-Disruption Library Offers Insight into Tn917 Insertion Patterns  

PubMed Central

Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TATAA, a 5-bp sequence that is duplicated during insertion. The regional insertion site preference at the chromosome terminus was not observed in another low-G+C gram-positive bacterium, Listeria monocytogenes, although the consensus insertion sequence was the same. The 8,865 Tn917 insertion sites sequenced in E. faecalis corresponded to only ?610 different open reading frames, far fewer than the predicted number of 2,400, assuming random insertion. There was no significant preference in orientation of the Tn917 insertions with either transcription or replication. Even though OG1RF has a smaller genome than strain V583 (2.8 Mb versus 3.2 Mb), the only E. faecalis strain whose sequence is in the public domain, over 10% of the Tn917 insertions appear to be in a OG1RF-specific sequence, suggesting that there are significant genomic differences among E. faecalis strains.

Garsin, Danielle A.; Urbach, Jonathan; Huguet-Tapia, Jose C.; Peters, Joseph E.; Ausubel, Frederick M.

2004-01-01

92

Genetic manipulations in Rhizobium meliloti utilizing two new transposon Tn 5 derivatives  

Microsoft Academic Search

Two derivatives of the prokaryotic transposon Tn5 were constructed in vitro. In Tn5-233, the central area of Tn5, which carries resistance to kanamycin\\/neomycin, bleomycin and streptomycin, is replaced by a fragment carrying resistance to the aminocyclitol antibiotics gentamycin\\/kanamycin and streptomycin\\/spectinomycin. In Tn5-235, the Escherichia coli ß-galactosidase gene is inserted within the streptomycin resistance gene of Tn5, and constitutively expressed from

Guido F. De Vos; Graham C. Walker; Ethan R. Signer

1986-01-01

93

Evaluation of TnT Tagger for Spanish  

Microsoft Academic Search

Part of speech (POS) tagger is a necessary module in many natural language text processing tasks. A POS tagger is a program that accepts an unprepared raw text in input and to each word adds a tag specifying its grammatical properties, such as part of speech, number, person, etc. One of popular POS taggers - TnT tagger - has been

Raul Morales Carrasco; Alexander F. Gelbukh

2003-01-01

94

Efficient chemoenzymatic synthesis of sialyl Tn-antigen and derivatives†  

PubMed Central

An N-terminal and C-terminal truncated recombinant ?2–6-sialyltransferase cloned from Photobacterium sp. JH-ISH-224, Psp2,6ST(15–501)-His6, was shown to be an efficient catalyst for one-pot three-enzyme synthesis of sialyl Tn (STn) antigens and derivatives containing natural and non-natural sialic acid forms.

Ding, Li; Yu, Hai; Lau, Kam; Li, Yanhong; Muthana, Saddam; Wang, Junru; Chen, Xi

2011-01-01

95

Tn 5 as a model for understanding DNA transposition  

Microsoft Academic Search

Summary Tn 5 is an excellent model system for understanding the molecular basis of DNA-mediated transposition. Mechanistic information has come from genetic and biochemical investigations of the transposase and its interactions with the recognition DNA sequences at the ends of the transposon. More recently, molecular structure analyses of catalytically active transposase; transposon DNA complexes have provided us with unprecedented insights

William S. Reznikoff

96

78 FR 36131 - Proposed Establishment of Class E Airspace; Cleveland, TN  

Federal Register 2010, 2011, 2012, 2013

...Proposed Establishment of Class E Airspace; Cleveland, TN AGENCY: Federal Aviation Administration...March 6, 2013, to establish Class E airspace at Cleveland Regional Jetport, Cleveland, TN, is being withdrawn. Upon review,...

2013-06-17

97

78 FR 14475 - Proposed Establishment of Class E Airspace; Cleveland, TN  

Federal Register 2010, 2011, 2012, 2013

...Proposed Establishment of Class E Airspace; Cleveland, TN AGENCY: Federal Aviation Administration...proposes to establish Class E Airspace at Cleveland, TN, to accommodate the Area Navigation...Standard Instrument Approach Procedures at Cleveland Regional Jetport. This action...

2013-03-06

98

Tn5 transposase-assisted transformation of indica rice.  

PubMed

Here, we describe experiments on Tn5 transposase-assisted transformation of indica rice. Transposomes were formed in vitro as a result of hyperactive Tn5 transposase complexing with a transposon that contained a 19-bp tetracycline operator (tetO) sequence. To form modified projectiles for transformation, the Tn10-derived prokaryotic tetracycline repressor (TetR) proteins, which can bind transposomes via the high affinity of TetR for tetO, were immobilized onto the surface of bare gold microscopic particles. These projectiles were introduced into cells of the indica rice cultivar Zhuxian B by particle bombardment. Once projectiles were inside the cell, tetracycline induced an allosteric conformational change in TetR that resulted in the dissociation of TetR from tetO, and thus generated free transposomes. Molecular evidence of transposition was obtained by the cloning of insertion sites from many transgenic plants. We also demonstrated that the introduced foreign DNA was inherited stably over several generations. This technique is a promising transformation method for other plant species as it is species independent. PMID:21635585

Wu, Jun; Du, Hongwei; Liao, Xuewei; Zhao, Yu; Li, Liguan; Yang, Liuyan

2011-07-18

99

Tn 5 carries a streptomycin resistance determinant downstream from the kanamycin resistance gene  

Microsoft Academic Search

In Rhizobium meliloti, Tn5 conferred resistance not only to kanamycin but to streptomycin, as well, in Escherichia coli, however only to kanamycin. Using in vitro recombinant DNA techniques, it was shown that the streptomycin resistance determinant was located downstream from the kanamycin resistance gene in the unique central region of Tn5. Expression of various cloned fragments of Tn5 suggested that

Peter Putnoky; György B. Kiss; Istvan Ott; Adam Kondorosi

1983-01-01

100

Renewed interest in basic and applied research involving monoclonal antibodies against an oncofetal Tn-antigen.  

PubMed

Tn-antigen (GalNAc?-Ser/Thr) is one of the most common aberrations associated with cancer progression and metastasis, and thus is an excellent target for development of cancer diagnostics and therapeutics. MLS128 monoclonal antibody (mAb), derived from a mouse immunized with human colon carcinoma cells, was reported to bind to two or three consecutive Tn-antigens (Tn2 or Tn3) with one-order higher affinity for Tn3 than for Tn2. Our recent studies demonstrated that MLS128 significantly inhibits breast and colon cancer cell growth. Molecular cloning of the variable regions of heavy (VH) and light (VL) chains revealed that the VH sequence of MLS128 shared 97% nucleotide sequence identity with the VH of 83D4 mAb, derived from breast cancer-immunized mice, which has a similar affinity for Tn2/Tn3. MLS128 single-chain antibodies (scFv) and scFv-Fc were constructed to confirm the affinity for synthetic Tn2/Tn3 peptides. Thermodynamic studies on MLS128 binding to Tn2/Tn3 revealed its unique nature of temperature-dependent binding. PMID:23740330

Fujita-Yamaguchi, Yoko

2013-06-04

101

mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320  

Microsoft Academic Search

We previously constructed a series of mini-Tn7 chromosome integration vectors that, when provided only with the site-specific transposition machinery, generally transpose to a naturally evolved, neutral attTn7 site that is located 25-bp downstream of the glmS gene. Here we provide a protocol for application of the mini-Tn7 system in Proteus mirabilis as an example of a bacterium with a secondary

Kyoung-Hee Choi; Herbert P Schweizer

2006-01-01

102

mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344  

Microsoft Academic Search

The mini-Tn7 vectors are universally applicable in Gram-negative bacteria and thereby facilitate the manipulation of many organisms for which few genetic systems are available. These vectors, when provided with only the Tn7 site-specific transposition machinery, insert site and orientation specifically in the bacterial chromosome at an attTn7 site downstream of the essential glmS gene. A few bacteria, including Burkholderia spp.,

Kyoung-Hee Choi; David DeShazer; Herbert P Schweizer

2006-01-01

103

Control of Neogene Extension in Idaho and Utah by Thrust Ramps in the Sevier Orogenic Belt: Evidence From Gravity Modeling in Cache and Cottonwood Valleys  

NASA Astrophysics Data System (ADS)

The subsurface structure of Cache and Cottonwood Valleys is largely due to two episodes of deformation. The Cretaceous to Eocene Sevier orogeny caused compressional folding and faulting across the region presently occupied by the basins. Following this, Neogene extension occurred along the Bannock detachment fault and other normal faults in Cache and Cottonwood Valleys. See-saw slippage of the west-dipping East Cache Valley fault and east-dipping West Cache fault in Utah and Idaho created a graben which filled with sediments of the Salt Lake Formation. Gravity modeling shows the subsurface structure of the basin using layer densities constrained by well logs and previously published densities. The base of the Tertiary was also constrained by seismic interpretations. The gravity model shows the location and dip of the East Cache Valley fault, West Cache fault, and Wasatch fault. It also indicates a ramp in the Paris thrust fault beneath the East Cache Valley fault and the Bear River Range (called the Logan ramp in this study), which puts higher density Proterozoic to middle Paleozoic units over lower density upper Paleozoic to Mesozoic units. In Cottonwood Valley, which is northeast of Cache Valley in Idaho, gravity data indicate that Neogene extension created a series of smaller northeast-dipping half grabens above the proposed breakaway zone of the southwest-dipping Bannock detachment fault. Like the East Cache Valley fault, the gravity data also indicate that the breakaway zone of the Bannock detachment fault in Cottonwood Valley formed above a ramp in the Paris thrust (called the Treasureton ramp in this and a previous study) which also placed higher density units over lower density ones. Based on the gravity and geologic data in this study, we interpret the East Cache Valley fault to be the breakaway zone for the Bannock detachment fault in southern and central Cache Valley. It is also likely that this fault connects to the Bannock detachment fault breakaway zone in Cottonwood Valley. Furthermore, we believe that the Logan and Treasureton ramps in the Paris thrust are connected, and that this ramp, as well as overlying thrust faults, were somehow responsible for localizing the breakaway zone of the Bannock detachment fault system tens of millions of years after thrusting ceased.

Groze, C. L.; Kruger, J. M.; Eversaul, M. L.

2006-12-01

104

A comparison of Anisotropy of Magnetic Susceptibility Studies to Clast Based Strain Analysis in Sandstones, From The Outer Margin Of The Sevier Orogenic Foreland, Western Wyoming.  

NASA Astrophysics Data System (ADS)

The Cordilleran Mountain Belt of North America is one of the world's classic foreland fold and thrust belts. The Sevier Belt represents the thin skinned front of this orogeny, consisting of thrust faults and folds that shortened and transported sequences of Devonian to Cretaceous strata eastward. There is a general increase in deformation westwards which provides an ideal geological setting to explore the potential link between Anisotropy of Magnetic Susceptibility (AMS) and results from clast based strain analyses of sandstones. Studies attempting to define the relationship between AMS and finite strain have been in vogue since the link between layer parallel shortening and AMS was first established. The understanding of this relationship, despite proven strong correlations between the AMS tensors and tectonic directions, is complicated by competing sub-fabrics, as well as the various magnetic properties of the minerals contributing to the AMS fabric. It has become very clear that the sensitivity of AMS is capable of detecting incipient tectonic fabrics, <5% shortening, which is typically outside the range of most dedicated strain analyses. Despite this, there has been little published research into the relationship between the classic fabric analysis techniques or strain estimate methods and AMS. This may be due to the particularly laborious task of calculating strain estimates using large marker populations and/or the pitfalls of trying to relate the magnitudes of the magnetic ellipse to the magnitudes of the strain ellipse. Rather than trying to estimate finite strain directly from the AMS ellipsoid, we are using the ability of AMS to accurately and quickly qualify the petrofabric and determine the origin of that fabric (i.e., whether it is purely sedimentary, composite bedding/tectonic or dominantly tectonic etc.). Where as most methods of estimating strain have poor accuracy constraints in low strain regimes. In an attempt to account for this AMS is being utilised as a control for the sensitivity limits for these strain analysis methods, this is in part due to low sample counts, subjectivity and human error. This research uses recently developed and remodified strain analysis methods that are being utilised in a Mathematica environment. The use of Mathematica allows for objective, swift, reproducible and automated calculation of a suite of strain estimate techniques, such as the Fry method, a variation of Ramsay's Nearest Neighbour Method and the Mean Radial Length Method.

Mc Carthy, Dave; Meere, Pat; Petronis, Mike; Mulchrone, Kieran

2013-04-01

105

IL17A-producing neutrophil-regulatory Tn lymphocytes  

Microsoft Academic Search

The proinflammatory cytokine IL-17A, mainly produced by specialized T cells, plays an important homeostatic role in regulating\\u000a neutrophil production and blood neutrophil counts. This review will assemble and discuss the evidence for this function of\\u000a IL-17A-producing cells, which are collectively called neutrophil-regulatory T cells or Tn cells. IL-17A-producing lymphocytes\\u000a are most abundant in the mesenteric lymph node, where they account

Klaus Ley; Emily Smith; Matthew A. Stark

2006-01-01

106

Evaluation of endosonography in TN staging of oesophageal cancer.  

PubMed Central

Strategies for the treatment of cancer of the oesophagus depend on the tumour stage at the time of diagnosis. Resection, the only curative treatment, is confined to early tumour stages. Tumours with local infiltration are usually unresectable and require palliative treatment. Computed tomography has been widely used for preoperative staging but often fails to define this correctly. Endoscopic ultrasound allows direct visualisation of the parietal wall and may be useful in staging gastrointestinal tumours. In a comparative prospective study, 52 patients with tumours of the oesophagus were investigated preoperatively both by endoscopic ultrasound and computed tomography to determine the stage of tumour infiltration and local lymph node involvement. Thirty seven of these patients underwent operation, resection, or dissection and entered the study. The intraoperative findings or the histopathological assessment, or both, were taken as a reference. For all TN stages of oesophageal tumours, correct preoperative staging was accomplished by endoscopic ultrasound in 89% for T stage and 69% for N stage compared with 51% and 51% respectively by computed tomography (highly significant using Fisher's exact test). This study shows that endoscopic ultrasound is useful in preoperative TN staging of tumours of the oesophagus. Images Figure 1 Figure 2

Ziegler, K; Sanft, C; Zeitz, M; Friedrich, M; Stein, H; Haring, R; Riecken, E O

1991-01-01

107

BWR - Spent Fuel Transport and Storage with the TN{sup TM}9/4 and TN{sup TM}24BH Casks  

SciTech Connect

The Swiss Nuclear Utilities have started in 2001 to store spent fuel in dry metallic dual-purpose casks at ZWILAG, the Swiss interim storage facility. BKW FMB Energy Ltd., the Muehleberg Nuclear Power Plant owner, is involved in this process and has elected to store its BWR spent fuel in a new high capacity dual-purpose cask, the TNeTeM24BH from the COGEMA Logistics/TRANSNUCLEAR TN{sup TM}24 family. The Muehleberg BWR spent fuels are transported by road in a medium size shuttle transport cask and then transferred to a heavy transport/storage cask (dry transfer) in the hot cell of ZWILAG site. For that purpose, COGEMA Logistics designed and supplied: - Two shuttle casks, TN{sup TM}9/4, mainly devoted to transport of spent fuel from Muehleberg NPP to ZWILAG. Licensed according to IAEA 1996, the TN{sup TM}9/4 is a 40 ton transport cask, for 7 BWR high bum-up spent fuel assemblies. - A series of new high capacity dual-purpose casks, TN{sup TM}24BH, holding 69 BWR spent fuels. Two transport campaigns took place in 2003 and 2004. For each campaign, ten TN{sup TM}9/4 round trips are performed, and one TN{sup TM}24BH is loaded. 5 additional TN{sup TM}24BH are being manufactured for BKW, and the next transport campaigns are scheduled from 2006. The TN{sup TM}24BH high capacity dual purpose cask and the TN{sup TM}9/4 transport cask characteristics and capabilities will then be detailed. (authors)

Wattez, L. [COGEMA LOGISTICS - AREVA Group (France); Marguerat, Y. [BKW FMB Energy Ltd (Switzerland); Hoesli, C. [ZWILAG Zwischenlager Wuerenlingen AG (Switzerland)

2006-07-01

108

4. Photocopy of May 21, 1936 photograph showing the house ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Photocopy of May 21, 1936 photograph showing the house (south front). The original photograph was taken by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8083. - Walker Family Farm, Big House, Gatlinburg, Sevier County, TN

109

9. Photocopy of photograph showing myrtlecovered spring. The original photograph ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Photocopy of photograph showing myrtle-covered spring. The original photograph was taken on May 21, 1936 by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8096. - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

110

8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY VIEW OF ABUTMENT SUPPORT INTO THE GROUND - Lost Creek Road Bridge, Spanning Sevier River at County Road 243, 2 miles east of Aurora, Aurora, Sevier County, UT

111

Transfer of Tn5385, a Composite, Multiresistance Chromosomal Element from Enterococcus faecalis  

Microsoft Academic Search

Tn5385 is a ca. 65-kb element integrated into the chromosomes of clinical Enterococcus faecalis strains CH19 and CH116. It confers resistance to erythromycin, gentamicin, mercuric chloride, streptomycin, tetracycline- minocycline, and penicillin via b-lactamase production. Tn5385 is a composite structure containing regions previously found in staphylococcal and enterococcal plasmids. Several transposons and transposon-like ele- ments within Tn5385 have been identified, including

LOUIS B. RICE; LENORE L. CARIAS

1998-01-01

112

Physical and functional mapping of Tn 2603 , a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance  

Microsoft Academic Search

A map of cleavage sites for restriction endonucleases EcoR1, BamHI, HindIII, and SalI on Tn2603, a transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury, was constructed by an analysis of restriction cleavage patterns of plasmid pMK1.:: Tn2603 and its deletion derivative. By cloning the fragments generated from pMK1::Tn2603 with these restriction endonucleases to a pACYC184 plasmid vehicle, the regions

Tomoko Yamamoto; Michiyasu Tanaka; Rieko Baba; Saburo Yamagishi

1981-01-01

113

TN approximation to neutron transport equation and application to critical slab problem  

NASA Astrophysics Data System (ADS)

The critical slab problem has been studied in one-speed neutron transport equation with isotropic scattering by using the TN method. TN moment criticality solutions are obtained for the uniform finite slab using Mark and Marshak type vacuum boundary conditions. Results obtained by TN method, using the two type boundary conditions mentioned above, were presented in the Tables and also the Tables included the results obtained by PN method for the comparisons.

Anli, Fikret; Ya?a, Faruk; Güngör, Süleyman; Öztürk, Hakan

2006-09-01

114

A bifunctional DNA binding region in Tn5 transposase  

PubMed Central

Tn5 transposition is a complicated process that requires the formation of a highly ordered protein–DNA structure, a synaptic complex, to catalyse the movement of a sequence of DNA (transposon) into a target DNA. Much is known about the structure of the synaptic complex and the positioning of protein–DNA contacts, although many protein–DNA contacts remain largely unstudied. In particular, there is little evidence for the positioning of donor DNA and target DNA. In this communication, we describe the isolation and analysis of mutant transposases that have, for the first time, provided genetic and biochemical evidence for the stage-specific positioning of both donor and target DNAs within the synaptic complex. Furthermore, we have provided evidence that some of the amino acids that contact donor DNA also contact target DNA, and therefore suggest that these amino acids help define a bifunctional DNA binding region responsible for these two transposase–DNA binding events.

Gradman, Richard J; Ptacin, Jerod L; Bhasin, Archna; Reznikoff, William S; Goryshin, Igor Y

2008-01-01

115

Recombination of knotted substrates by Tn3 resolvase.  

PubMed Central

We studied the site orientation specificity for recombination by purified Tn3 resolvase. With standard plasmid substrates, resolvase acts only on directly repeated recombination sites. Knotting, however, makes inverted site substrates equally efficient. The structure of the knotted products of recombination shows that the DNA wrapped around resolvase in the synaptic intermediate has the same local geometry as the standard substrate but is reversed in topological sign. Similarly, the same strand exchange with the two substrates generates supercoils with opposite signs. Thus, DNA geometry rather than topology is critical for these features of recombination. The knotted inverse substrate like the direct site substrate must be (-) supercoiled under standard reaction conditions. However, under conditions in which supercoiling is not required, the structure of the knotted product is apparently the same. This indicates that the unique direction of strand exchange is determined by the structure of the synaptosome and not by (-) supercoiling of the substrate. Images

Droge, P; Cozzarelli, N R

1989-01-01

116

Transposon mutagenesis in Actinobacillus pleuropneumoniae with a Tn10 derivative.  

PubMed

A transposon mutagenesis procedure functional in the gram-negative swine pathogen Actinobacillus pleuropneumoniae was developed for the first time. The technique involved the use of a suicide conjugative plasmid, pLOF/Km, carrying a mini-Tn10 with an isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible transposase located outside the mobile element (M. Herrero, V. de Lorenzo, and K. N. Timmis, J. Bacteriol. 172:6557-6567, 1990). The plasmid was mobilized from Escherichia coli to A. pleuropneumoniae through the RP4-mediated broad-host-range conjugal transfer functions provided by the chromosome of the donor strain. When IPTG was present in the mating medium, A. pleuropneumoniae CM5 transposon mutants were obtained at a frequency of 10(-5), while no mutants were detected in the absence of IPTG. Since the frequency of conjugal transfer of the RP4 plasmid from E. coli to A. pleuropneumoniae CM5 was found to be as low as 10(-4), the above result indicated that the expression level of the transposase was a critical factor for obtaining a workable efficiency of transposon mutagenesis. The transposon insertions occurred at random, as determined by Southern blotting of chromosomal DNA of randomly selected mutants and by the ability to generate mutants defective for the selected phenotypes. Almost all the mutants analyzed resulted from a single insertion of the Tn10 element. About 1.2% of the mutants resulted from the cointegration of pLOF/Km into the A. pleuropneumoniae chromosome. The applicability of this transposon mutagenesis system was verified on other A. pleuropneumoniae strains of different serotypes. The usefulness of this transposon mutagenesis system in genetic studies of A. pleuropneumoniae is discussed. PMID:8396122

Tascon, R I; Rodriguez-Ferri, E F; Gutierrez-Martin, C B; Rodriguez-Barbosa, I; Berche, P; Vazquez-Boland, J A

1993-09-01

117

Partial Excision of blaKPC from Tn4401 in Carbapenem-Resistant Klebsiella pneumoniae  

PubMed Central

We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial blaKPC fragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding blaKPC is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic.

Chen, Liang; Chavda, Kalyan D.; Mediavilla, Jose R.; Jacobs, Michael R.; Levi, Michael H.; Bonomo, Robert A.

2012-01-01

118

Nonlinear pressure dependence of TN in almost multiferroic EuTiO3.  

PubMed

The antiferromagnetic (AFM) phase transition temperature TN of EuTiO3 has been studied as a function of pressure p. The data reveal a nonlinear dependence of TN on p with TN increasing with increasing pressure. The exchange interactions exhibit an analogous dependence on p as TN (if the absolute value of the nearest neighbor interaction is considered) and there is evidence that the AFM transition is robust with increasing pressure. The corresponding Weiss temperature ?W remains anomalous since it always exhibits positive values. The data are analyzed within the Bloch power law model and provide excellent agreement with experiment. PMID:23963024

Guguchia, Z; Caslin, K; Kremer, R K; Keller, H; Shengelaya, A; Maisuradze, A; Bettis, J L; Köhler, J; Bussmann-Holder, A; Whangbo, M-H

2013-08-21

119

Nonlinear pressure dependence of TN in almost multiferroic EuTiO3  

NASA Astrophysics Data System (ADS)

The antiferromagnetic (AFM) phase transition temperature TN of EuTiO3 has been studied as a function of pressure p. The data reveal a nonlinear dependence of TN on p with TN increasing with increasing pressure. The exchange interactions exhibit an analogous dependence on p as TN (if the absolute value of the nearest neighbor interaction is considered) and there is evidence that the AFM transition is robust with increasing pressure. The corresponding Weiss temperature ?W remains anomalous since it always exhibits positive values. The data are analyzed within the Bloch power law model and provide excellent agreement with experiment.

Guguchia, Z.; Caslin, K.; Kremer, R. K.; Keller, H.; Shengelaya, A.; Maisuradze, A.; Bettis, J. L., Jr.; Köhler, J.; Bussmann-Holder, A.; Whangbo, M.-H.

2013-09-01

120

Effect of Tn10/Tn5 transposons on the survival and mutation frequency of halogen light-irradiated AB1157 Escherichia coli K-12.  

PubMed

We show here that the Tn10/Tn5 transposon when inserted into the chromosome of strain AB1157 makes the bacteria more sensitive to and less mutable by halogen light irradiation. These effects are most probably caused by depletion of UmuD and UmuC proteins since: (i) transformation of the transposon-bearing bacteria with plasmids harbouring umuD'C (or umuDC) leads to recovery of the original survival and mutation frequencies; (ii) insertion of Tn10/Tn5 into chromosomal DNA has no effect on the level of mutation induced by ethyl methane-sulphonate treatment, a mutagen whose activity is umuDC-independent; (iii) the decline in survival is in about the same range for Tn10-bearing bacteria as for bacteria with deleted umuDC. However, whereas transformation of bacteria deleted in umuDC with plasmids carrying umuD'C/umuDC leads to full recovery of halogen light-induced mutability, recovery of survival is poor. This suggests that the mechanisms leading to umuDC-dependent mutagenesis and umuDC-dependent protection of cell survival are different. None of these effects occurs in bacteria bearing the Tn9 transposon in their DNA. PMID:10474834

Wójcik, A; Janion, C

1999-01-01

121

Interactions between sediment resuspension and sediment quality decrease the TN : TP ratio in a shallow lake  

Microsoft Academic Search

The relative contributions of sediment resuspension and external nutrient loading to seasonal fluctuations of the total nitrogen to total phosphorus (TN : TP) ratio in the water column of the shallow Kirkkojarvi basin were studied. In May, the TN : TP mass ratio was above 30, but it decreased to below 10 in July-August. In October, the ratio increased again.

Juha Niemisto; Heidi Holmroos; Zeynep Pekcan-Hekim; Jukka Horppila

2008-01-01

122

Effects of deletions in transposon Tn7 on its frequency of transposition.  

PubMed Central

Deletions in transposon Tn7 either abolished transposition or reduced transposition frequency. Except for a deletion in the right-hand terminus, these deletions could be complemented in trans. A 2.1-kilobase fragment of Tn7 encodes a diffusible gene product which stimulates transposition above the wild-type frequency. No cointegrate formation was detected.

Smith, G M; Jones, P

1984-01-01

123

Immunization of breast cancer patients using a synthetic sialyl-Tn glycoconjugate plus Detox adjuvant  

Microsoft Academic Search

We have synthesized various formulations that have potential for active specific immunotherapy (ASI) of human cancers. Sialyl-Tn (STn) is a potentially important target structure for ASI because its expression on mucins is a strong, independent predictor of poor prognosis, suggesting that it may have functional significance in the metastatic process. In this first pilot study of synthetic sialyl-Tn hapten conjugated

Grant D. MacLean; Mark Reddish; R. Rao Koganty; Ting Wong; Sham Gandhi; Mark Smolenski; John Samuel; Jean Marc Nabholtzl; B. Michael Longenecker

1993-01-01

124

76 FR 35370 - Proposed Establishment of Class E Airspace; Copperhill, TN  

Federal Register 2010, 2011, 2012, 2013

...instrument approach procedures for Martin Campbell Field Airport. Controlled...establish Class E airspace at Martin Campbell Field Airport, Copperhill...DESIGNATION OF CLASS A, B, C, D, AND E AIRSPACE AREAS; AIR...TN E5 Copperhill, TN [New] Martin Campbell Field Airport...

2011-06-17

125

76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY  

Federal Register 2010, 2011, 2012, 2013

...South Fork National Recreation Area, TN/KY AGENCY: National Park...South Fork National Recreation Area, TN/KY...SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby...South Fork National Recreation Area, Tennessee and Kentucky,...

2011-06-20

126

78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN  

Federal Register 2010, 2011, 2012, 2013

...13-ASO-15] Amendment of Class E Airspace; Tri-Cities, TN AGENCY: Federal Aviation...This action amends Class E airspace for the Tri-Cities, TN area, by correcting the regulatory text of the Class E surface airspace at Tri-Cities Regional Airport,...

2013-07-23

127

TN approximation to reflected slab and computation of the critical half thicknesses  

NASA Astrophysics Data System (ADS)

The criticality solution to one-speed neutron transport equation using the TN approximation is described for reflected slab. In the solution, Marshak type boundary condition is used. The critical half thicknesses are computed for different values of c and reflection coefficients. Computations are made by using the both TN and PN approximation for the comparison.

Anli, F.; Güngör, S.; Ya?a, F.; Öztürk, H.

2006-09-01

128

International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21?  

PubMed Central

TEM-24 remains one of the most widespread TEM-type extended-spectrum ?-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-?Tn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly penetrating clones and highly promiscuous plasmids in the spread of antibiotic resistance, and it contributes to the elucidation of the origin and evolution of TEM-2 ESBL derivatives.

Novais, Angela; Baquero, Fernando; Machado, Elisabete; Canton, Rafael; Peixe, Luisa; Coque, Teresa M.

2010-01-01

129

International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21.  

PubMed

TEM-24 remains one of the most widespread TEM-type extended-spectrum beta-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-DeltaTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly penetrating clones and highly promiscuous plasmids in the spread of antibiotic resistance, and it contributes to the elucidation of the origin and evolution of TEM-2 ESBL derivatives. PMID:19995930

Novais, Angela; Baquero, Fernando; Machado, Elisabete; Cantón, Rafael; Peixe, Luísa; Coque, Teresa M

2009-12-07

130

Tn6188 - A Novel Transposon in Listeria monocytogenes Responsible for Tolerance to Benzalkonium Chloride.  

PubMed

Controlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S.) aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC) as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR) transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs) (28.5 ± 4.7 mg/l) than strains without Tn6188 (14 ± 3.2 mg/l). Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that ?qacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L. monocytogenes strains. PMID:24098567

Müller, Anneliese; Rychli, Kathrin; Muhterem-Uyar, Meryem; Zaiser, Andreas; Stessl, Beatrix; Guinane, Caitriona M; Cotter, Paul D; Wagner, Martin; Schmitz-Esser, Stephan

2013-10-02

131

Tn6188 - A Novel Transposon in Listeria monocytogenes Responsible for Tolerance to Benzalkonium Chloride  

PubMed Central

Controlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S.) aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC) as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR) transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs) (28.5 ± 4.7 mg/l) than strains without Tn6188 (14 ± 3.2 mg/l). Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that ?qacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L. monocytogenes strains.

Muhterem-Uyar, Meryem; Zaiser, Andreas; Stessl, Beatrix; Guinane, Caitriona M.; Cotter, Paul D.; Wagner, Martin; Schmitz-Esser, Stephan

2013-01-01

132

Adult teleost heart expresses two distinct troponin C paralogs: cardiac TnC and a novel and teleost-specific ssTnC in a chamber- and temperature-dependent manner.  

PubMed

The teleost-specific whole genome duplication created multiple copies of genes allowing for subfunctionalization of isoforms. In this study, we show that the teleost cardiac Ca(2+)-binding troponin C (TnC) is the product of two distinct genes: cardiac TnC (cTnC, TnnC1a) and a fish-specific slow skeletal TnC (ssTnC, TnnC1b). The ssTnC gene is novel to teleosts as mammals have a single gene commonly referred as cTnC but which is also expressed in slow skeletal muscle. In teleosts, the data strongly indicate that these are two TnC genes are different paralogs. Because we determined that ssTnC exists across many teleosts but not in basal ray-finned fish (e.g., bichir), we propose that these paralogs are the result of an ancestral tandem gene duplication persisting only in teleosts. Quantification of mRNA levels was used to demonstrate distinct expression localization patterns of the paralogs within the chambers of the heart. In the adult zebrafish acclimated at 28°C, ssTnC mRNA levels are twofold greater than cTnC mRNA levels in the atrium, whereas cTnC mRNA was almost exclusively expressed in the ventricle. Meanwhile, rainbow trout acclimated at 5°C showed cTnC mRNA levels in both chambers significantly greater than ssTnC. Distinct responses to temperature acclimation were also quantified in both adult zebrafish and rainbow trout, with mRNA in both chambers shifting to express higher levels of cTnC in 18°C acclimated zebrafish and 5°C acclimated trout. Possible subfunctionalization of TnC isoforms may provide insight into how teleosts achieve physiological versatility in chamber-specific contractile properties. PMID:23881286

Genge, Christine E; Davidson, William S; Tibbits, Glen F

2013-07-23

133

Spectroscopic and ITC study of the conformational change upon Ca{sup 2+}-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle  

SciTech Connect

Akazara scallop (Chlamys nipponensis akazara) troponin C (TnC) of striated adductor muscle binds only one Ca{sup 2+} ion at the C-terminal EF-hand motif (Site IV), but it works as the Ca{sup 2+}-dependent regulator in adductor muscle contraction. In addition, the scallop troponin (Tn) has been thought to regulate muscle contraction via activating mechanisms that involve the region spanning from the TnC C-lobe (C-lobe) binding site to the inhibitory region of the TnI, and no alternative binding of the TnI C-terminal region to TnC because of no similarity between second TnC-binding regions of vertebrate and the scallop TnIs. To clarify the Ca{sup 2+}-regulatory mechanism of muscle contraction by scallop Tn, we have analyzed the Ca{sup 2+}-binding properties of the complex of TnC C-lobe and TnI peptide, and their interaction using isothermal titration microcalorimetry, nuclear magnetic resonance, circular dichroism, and gel filtration chromatography. The results showed that single Ca{sup 2+}-binding to the Site IV leads to a structural transition not only in Site IV but also Site III through the structural network in the C-lobe of scallop TnC. We therefore assumed that the effect of Ca{sup 2+}-binding must lead to a change in the interaction mode between the C-lobe of TnC and the TnI peptide. The change should be the first event of the transmission of Ca{sup 2+} signal to TnI in Tn ternary complex.

Yumoto, Fumiaki [Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan); Department of Physiology II, Jikei University School of Medicine, Tokyo (Japan); Tanaka, Hiroyuki [Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido (Japan); Nagata, Koji; Miyauchi, Yumiko; Miyakawa, Takuya [Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan); Ojima, Takao [Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido (Japan); Tanokura, Masaru [Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)], E-mail: amtanok@mail.ecc.u-tokyo.ac.jp

2008-04-25

134

Corynebacterium striatum chloramphenicol resistance transposon Tn5564: genetic organization and transposition in Corynebacterium glutamicum.  

PubMed

The clinical isolate Corynebacterium striatum M82B (formerly Corynebacterium xerosis M82B) carries the 50-kb R-plasmid pTP10 conferring resistance to the antibiotics chloramphenicol, erythromycin, kanamycin, and tetracycline. DNA sequence analysis of the chloramphenicol resistance region revealed the presence of the 4155-bp transposable element Tn5564. The ends of Tn5564 are identical 22-bp inverted repeats flanked by a 6-bp target site duplication. The central region of Tn5564 encodes the chloramphenicol resistance gene cmx, specifying a transmembrane chloramphenicol efflux protein, and an open reading frame homologous to transposases of insertion sequences identified in Arthrobacter nicotinovorans and Bordetella pertussis. Furthermore, the 1715-bp insertion sequence IS1513 encoding a putative transposase of the IS30 family is an integral part of Tn5564 and is located upstream of cmx. For transposon mutagenesis, Tn5564 was transferred to Corynebacterium glutamicum on a mobilizable Escherichia coli plasmid using RP4-mediated intergeneric conjugation. Transposition of Tn5564 in C. glutamicum occurred with a frequency of 3.3 x 10(-8) and resulted in an insertion into target sites containing the central palindromic tetranucleotide CTAG. A Tn5564-induced mutant strain of C. glutamicum was found to carry the transposon in the ftsZ gene region. PMID:9735314

Tauch, A; Zheng, Z; Pühler, A; Kalinowski, J

1998-09-01

135

Genetic analysis of the Tn21 mer operator-promoter.  

PubMed Central

The mercury resistance operon, mer, of the transposon Tn21 is transcribed from two overlapping divergent promoters: PR for the regulatory gene, merR, and PTPCAD for the structural genes, merTPCAD. Transcription of merTPCAD is repressed in the absence of Hg(II) and activated in the presence of Hg(II) by the regulatory protein, MerR. In addition, MerR represses its own expression regardless of the presence of Hg(II). MerR binds as a dimer to a single region of dyad symmetry lying between the -35 and -10 hexamers of PTPCAD. Analysis of the expression of transcriptional fusions to hydroxylamine- and oligonucleotide-generated mutants of this divergent operator-promoter region identified key bases involved in MerR-dependent repression of PTPCAD and of PR and in activation of PTPCAD. Six of the seven mutants affecting the palindromic region were altered in their ability to bind the MerR protein in vitro as measured by fragment retardation assays. These differences in in vitro MerR binding correlated well with the in vivo measurements of repression or of activation. Bases identified as functionally relevant by this genetic analysis coincide extensively with those previously identified as relevant via in vivo footprinting. Four major points emerge from this analysis: (i) transition and transversion mutations within the spacer between the -10 and -35 hexamers of PTPCAD generally have little effect on the MerR-independent (i.e., unregulated) expression of either promoter; (ii) alteration of certain bases in the MerR-binding dyad affects repression of PTPCAD differently than repression of PR; (iii) certain dyad changes can impair activation of PTPCAD more severely than repression of this promoter; and (iv) mutations in the -10 hexamer of PTPCAD which also effect PR expression define one of two potential -10 hexamers in PR as actually functional in vivo. Images

Park, S J; Wireman, J; Summers, A O

1992-01-01

136

The development of Tn Nuc and its use for the isolation of novel secretion signals in Lactococcus lactis  

Microsoft Academic Search

We have previously used Tn917 for the identification and characterization of regulated promoters from Lactococcus lactis [Israelsen et al., Appl. Environ. Microbiol. 61 (1995) 2540–2547]. We describe here the construction of a new Tn917-transposon derivative, termed TnNuc, which includes the Staphylococcus aureus nuclease gene (nuc) as a reporter for secretion. Transposition of TnNuc into the L. lactis chromosome allows the

Peter Ravn; José Arnau; Søren M. Madsen; Astrid Vrang; Hans Israelsen

2000-01-01

137

Photo-Addressed Liquid Crystal SLM with Twisted Nematic ECB (TN-ECB) Mode  

NASA Astrophysics Data System (ADS)

A novel photo-addressed LC-SLM featuring TN-ECB (twisted nematic electrically controlled birefringence) is presented. The LC-SLM consists of an a-Si photoconductor, a dielectric mirror and a TN-ECB liquid crystal layer. TN-ECB is a kind of ECB mode which has been developed by simulating light propagation in a reflective twisted nematic configuration. The features of the LC-SLM are 25 lp/mm resolution, high-speed response of less than 10 ms, and a single polarizer system. The resolution limitation by the dielectric mirror is also discussed.

Sonehara, Tomio

1990-07-01

138

Inhibition of fungi and gram-negative bacteria by bacteriocin BacTN635 produced by Lactobacillus plantarum sp. TN635.  

PubMed

The aim of this study was to evaluate 54 lactic acid bacteria (LAB) strains isolated from meat, fermented vegetables and dairy products for their capacity to produce antimicrobial activities against several bacteria and fungi. The strain designed TN635 has been selected for advanced studies. The supernatant culture of this strain inhibits the growth of all tested pathogenic including the four Gram-negative bacteria (Salmonella enterica ATCC43972, Pseudomonas aeruginosa ATCC 49189, Hafnia sp. and Serratia sp.) and the pathogenic fungus Candida tropicalis R2 CIP203. Based on the nucleotide sequence of the 16S rRNA gene of the strain TN635 (1,540 pb accession no FN252881) and the phylogenetic analysis, we propose the assignment of our new isolate bacterium as Lactobacillus plantarum sp. TN635 strain. Its antimicrobial compound was determined as a proteinaceous substance, stable to heat and to treatment with surfactants and organic solvents. Highest antimicrobial activity was found between pH 3 and 11 with an optimum at pH = 7. The BacTN635 was purified to homogeneity by a four-step protocol involving ammonium sulfate precipitation, centrifugal microconcentrators with a 10-kDa membrane cutoff, gel filtration Sephadex G-25, and C18 reverse-phase HPLC. SDS-PAGE analysis of the purified BacTN635, revealed a single band with an estimated molecular mass of approximately 4 kDa. The maximum bacteriocin production (5,000 AU/ml) was recorded after a 16-h incubation in Man, Rogosa, and Sharpe (MRS) medium at 30 degrees C. The mode of action of the partial purified BacTN635 was identified as bactericidal against Listeria ivanovii BUG 496 and as fungistatic against C. tropicalis R2 CIP203. PMID:19888697

Smaoui, Slim; Elleuch, Lobna; Bejar, Wacim; Karray-Rebai, Ines; Ayadi, Imen; Jaouadi, Bassem; Mathieu, Florence; Chouayekh, Hichem; Bejar, Samir; Mellouli, Lotfi

2009-11-04

139

Conjugal transfer of Tn916, Tn916 delta E, and pAM beta 1 from Enterococcus faecalis to Butyrivibrio fibrisolvens strains.  

PubMed Central

Anaerobic filter matings of Butyrivibrio fibrisolvens H17c, CF3, D1, or GS113, representing different DNA relatedness groups, were done with Enterococcus faecalis CG110, which contains chromosomally inserted Tn916. Tetracycline-resistant transconjugants were obtained with each mating pair at average frequencies of 4.4 x 10(-6) (per recipient) and 5.2 x 10(-6) (per donor). The transfer frequencies of Tn916 into B. fibrisolvens varied 5- to 10-fold with mating time, strain, and growth stage. By using Southern hybridization with pAM120 as the probe, Tn916 was shown to insert at one or more separate chromosomal sites for each strain of B. fibrisolvens. Retransfer of Tn916 from B. fibrisolvens H17c or CF3 to E. faecalis OG1-X or JH 2-2 or to B. fibrisolvens D1 or GS113 could not be shown. Matings of E. faecalis RH110, which contains chromosomally inserted Tn916 delta E, with B. fibrisolvens 49, H17c, D1, CF3, GS113, or VV-1 resulted in erythromycin-resistant transconjugants at average frequencies of 5.3 x 10(-7) (per recipient) and 2.5 x 10(-7) (per donor). Tn916 delta E was shown by Southern hybridization with pAM120 to insert at one or more sites in the chromosome of each strain. B. fibrisolvens H17c was anaerobically filter mated with E. faecalis JH 2-SS, which contains pAM beta 1. Erythromycin-resistant transconjugants were obtained at frequencies of 2 x 10(-5) (per recipient) and 6 x 10(-5) (per donor). The presence of pAM beta 1 in these transconjugants could not be shown by agarose gel electrophoresis of plasmid minilysates but could be shown by Southern hybridization analysis.(ABSTRACT TRUNCATED AT 250 WORDS) Images

Hespell, R B; Whitehead, T R

1991-01-01

140

78 FR 16202 - Proposed Amendment of Class E Airspace; Selmer, TN  

Federal Register 2010, 2011, 2012, 2013

...Approach Procedures have been developed at Robert Sibley Airport. This action would enhance...Instrument Approach Procedures developed at Robert Sibley Airport, Selmer, TN. The geographic coordinates of Robert Sibley Airport would be adjusted to...

2013-03-14

141

Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii.  

PubMed

Acinetobacter baumannii is an emerging pathogen that causes serious infections with high mortality rates in immunocompromised individuals. Genetic manipulations in this medically-relevant pathogen are limited by the paucity of molecular tools. In this study, we show the application of the mini-Tn7-based single copy insertion system in A. baumannii. Mini-Tn7 elements are known to integrate at a naturally evolved, therefore presumably neutral location (intergenic region) downstream of the glmS gene (glucosamine-fructose-6-phosphate aminotransferase) in Gram-negative bacteria. We identified the site of insertion of mini-Tn7 in A. baumannii and demonstrated application of this useful cloning tool by inserting the gfp gene into the chromosome. Our work shows that mini-Tn7 elements are useful tools for genetic studies in this important pathogen. PMID:20638421

Kumar, Ayush; Dalton, Courtney; Cortez-Cordova, Jenny; Schweizer, Herbert P

2010-07-16

142

Immunoreactive T and Tn epitopes in cancer diagnosis, prognosis, and immunotherapy  

Microsoft Academic Search

Aberrant glycosylation is a hallmark of cancer cells. The blood group precursors T (Thomsen-Friedenreich) and Tn epitopes\\u000a are shielded in healthy and benign-diseased tissues but uncovered in approx. 90% of carcinomas. T and Tn glycoproteins are\\u000a specific, autoimmunogenic pancarcinoma antigens. These antigens may also be found in neoplastic blood cells (and on LTV-II\\u000a infected T lymphocytes). Fundamental chemical and physical

Georg F. Springer

1997-01-01

143

Interactions of Tn7 and temperate phage F116L of Pseudomonas aeruginosa  

Microsoft Academic Search

Tn7 insertions into the genome of F116L, a Pseudomonas aeruginosa generalized transducing phage, were isolated by repeated cycles of transducing phage, were of strains lysogenic for F116cts mutants with selection for trimethoprim resistance (Tp1). Two non-defective F116Lcts:Tn7 phage were characterized. They have reduced plaquing ability, produced non-lysogenic Tpr transductants, and have yielded a deletion mutant of the phage genome upon

M. Caruso; J. A. Shapiro

1982-01-01

144

Analysis of a heterogeneous group of human breast carcinoma associated glycoproteins bearing the Tn determinant  

Microsoft Academic Search

The Tn determinant (GalNAca-O-Ser\\/Thr) is expressed by about 90% of human carcinomas, but is cryptic in most normal human tissues. A murine monoclonal antibody (MAb) 83D4, developed following immunization with human breast carcinoma cells, reacts with a Tn-related epitope. In the present study we characterized the glycoprotein antigen identified by 83D4 in the human breast carcinoma cell line MCF-7. We

Eduardo Osinaga; Gianfranco Pancino; Nicole Porchet; Nora Berois; Patricia De Cremoux; Dominique Mistro; Jean Pierre Aubert; Fabian Calvo; Alberto Roseto

1994-01-01

145

Analysis of hemolytic determinants of plasmid pHly185 by Tn5 mutagenesis.  

PubMed Central

A nonconjugative hemolysin plasmid, pHly185, was isolated from Escherichia coli 185. Tn5 mutagenesis produced nonhemolytic derivatives that either expressed no hemolytic activity or did not secrete activity. These Tn5 insertions were located in three contiguous EcoRI fragments. Insertions inactivating hemolysin structural gene(s) were identified on all three EcoRI fragments. Mutations affecting the secretory function were clustered in one portion of a single EcoRI fragment. Images

Stark, J M; Shuster, C W

1982-01-01

146

Enterococcal transposon Tn5384: evolution of a composite transposon through cointegration of enterococcal and staphylococcal plasmids.  

PubMed

Mechanisms for the possible transfer of antimicrobial resistance genes between staphylococci and enterococci remain poorly defined. We have previously reported the transfer between Enterococcus faecalis strains of a multiresistance chromosomal element (beta-lactamase positive and resistance to erythromycin, gentamicin, mercuric chloride, streptomycin, and tetracycline) which we have tentatively designated Tn5385. Tn5385 is a composite of several smaller transposable elements, including Tn5384, a 26-kb composite transposon conferring resistance to erythromycin, gentamicin, and mercuric chloride. Analyses of 7 kb within Tn5384 and flanking sequences within the larger element revealed sequences characteristic of staphylococcal beta-lactamase and small, mobilizable plasmids flanking a region with a sequence identical to those of the replication genes previously described for enterococcal and streptococcal broad-host-range plasmids. These diverse regions are linked by insertion sequences IS256 and IS257 in a manner which suggests a series of cointegration events as the genesis of the current relationship. Taken together, these data suggest that Tn5384 and the larger element within which it is incorporated (Tn5385) evolved at least in part as a result of cointegration between an enterococcal broad-host-range plasmid and staphylococcal beta-lactamase and small mobilizable plasmids. These results implicate broad-host-range plasmids in the transfer of resistance determinants from staphylococci to enterococci. PMID:9303373

Bonafede, M E; Carias, L L; Rice, L B

1997-09-01

147

Tn7 Transposition Creates a Hotspot for Homologous Recombination at the Transposon Donor Site  

PubMed Central

Homologous recombination at the bacterial transposon Tn7 donor site is stimulated 10-fold when Tn7 is activated to transpose at high frequency in RecD(-) Escherichia coli, where recombination is focused near the ends of double-chain breaks. This is observed as an increase in recombination between two lacZ heteroalleles when one copy of lacZ carries within it a Tn7 that is transposing at high frequency. This stimulation of recombination is dependent upon the presence of homology with the donor site, is independent of SOS induction, and is not due to a global stimulation of recombination. When stimulated by Tn7 transposition, the conversion events giving rise to Lac(+) recombinants occur preferentially at the site of Tn7, suggesting that transposition is stimulating gene conversion at the donor site. These results support the model that Tn7 transposition occurs by a ``cut and paste'' mechanism, leaving a double-chain break at the donor site that is repaired by the host homologous recombination machinery; normally, repair would use homology in a sister chromosome to regenerate a copy of the transposon. This proposed series of events allows transposition that is nonreplicative, per se, to be effectively replicative.

Hagemann, A. T.; Craig, N. L.

1993-01-01

148

A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production.  

PubMed

This study aimed to determine the probiotic potential of 100 strains of Lactic Acid Bacteria (LAB) isolated from different intestinal segments of indigenous poultry in Tunisia. The strains were submitted to a battery of standard tests and criteria commonly used for determining their probiotic properties and attributes. The findings revealed that 19 of the isolates exhibited antimicrobial activity against 4 pathogenic bacteria, and that 4 (TN1, TN8, TN7, and TN13) showed good resistance to pH 3 and 5% bovine bile. Three isolates, namely TN1, TN8, and TN13, showed sensitivity to several antibiotics and were, therefore, selected for further enzymatic activity assays. Two isolates, namely TN1 and TN8, showed high efficacy of adhesion to chicken enterocytes. The cytokines released after stimulation by the two isolates showed high anti-inflammatory profiles, with an increased rate of Interleukin-10 (IL-10) production for the TN8 strain. Showing the highest performance, TN8 was submitted to 16S rRNA gene sequencing, which revealed that the strain was of the species Lactobacillus plantarum. Overall, the findings indicate that the Lactobacilli from poultry intestine has a number of promising properties that make it candidate for application as a probiotic additive in poultry industry. PMID:22634330

Ben Salah, Riadh; Trabelsi, Imen; Ben Mansour, Riadh; Lassoued, Saloua; Chouayekh, Hichem; Bejar, Samir

2012-05-22

149

Geological and petrophysical characterization of the Ferron Sandstone for 3-D simulation of a fluvial-deltaic reservoir. Deliverable 1.4.4: Ferron Sandstone lithofacies and case-study areas, Emery and Sevier Counties, Utah  

SciTech Connect

The types of dominantly sandstone lithofacies that characterize the Cretaceous Ferron Sandstone reservoir types were tentatively identified before the project began. These reservoir types were defined and mapped at the regional scale and are the subject of the detailed, highly focused case studies. The purpose of conducting detailed case-study analysis is to develop a comprehensive, interdisciplinary, and quantitative characterization of fluvial-deltaic reservoirs which will allow realistic inter-well and reservoir-scale modeling to be used for improved oil-field development in actual reservoirs world-wide. The resulting benefits and value may: (1) increase recoverable reserves by identifying untapped compartments created by reservoir heterogeneity, (2) reduce development costs by more efficiently siting infill drilling locations, (3) increase deliverability by exploiting the reservoir along optimal fluid-flow paths, (4) enhance the application of new technologies, such as horizontal drilling, by identifying optimal drilling directions to maximize fluid-flow, and (5) identify reservoir trends for field extension drilling. Various geologic studies of the Ferron Sandstone were reviewed to compile a list of locations and types of lithofacies in the Ferron Sandstone to be examined in greater detail as part of the subsequent case studies. Preliminary regional interpretations were also used to help select the type and location of lithofacies for case studies. Potential case-study sites were delineated during several reconnaissance field trips by the geologic team. Two case-study sites were selected for the project: Ivie Creek and Willow Springs Wash, in the central and southern parts respectively of the project study area. Results are discussed.

Allison, M.L.

1996-01-04

150

County Home Rule.  

National Technical Information Service (NTIS)

Contents: The home rule concept; What can home rule do for counties; Criticisms of home rule; The ideals of county government; Legislative benefits of home rule; Home rule answers Arizona's problems; If home rule fails to be adopted.

1973-01-01

151

Fis plays a role in Tn5 and IS50 transposition.  

PubMed Central

The Fis (factor for inversion stimulation) protein of Escherichia coli was found to influence the frequency of transposon Tn5 and insertion sequence IS50 transposition. Fis stimulated both Tn5 and IS50 transposition events and also inhibited IS50 transposition in Dam-bacteria. This influence was not due to regulation by Fis of the expression of the Tn5 transposition proteins. We localized, by DNase I footprinting, one Fis site overlapping the inside end of IS50 and give evidence to strongly suggest that when Fis binds to this site, IS50 transposition is inhibited. The Fis site at the inside end overlaps three Dam GATC sites, and Fis bound efficiently only to the unmethylated substrate. Using a mobility shift assay, we also identified another potential Fis site within IS50. Given the growth phase-dependent expression of Fis and its differential effect on Tn5 versus IS50 transposition in Dam-bacteria, we propose that the high levels of Fis present during exponential growth stimulate transposition events and might bias those events toward Tn5 and away from IS50 transposition. Images

Weinreich, M D; Reznikoff, W S

1992-01-01

152

Mucin 6 and Tn antigen expression in canine mammary tumours: correlation with pathological features.  

PubMed

Overexpression of mucins is known to decrease cell-to-cell adhesion and thus to facilitate the invasion of cancer cells through the extracellular matrix. Mucin 6 (MUC6) is overexpressed and aberrantly O-glycosylated in human breast cancer, serving as a carrier for one of the most specific cancer-associated antigens, Tn antigen. Despite its relevance in breast cancer, MUC6 expression has not yet been characterized in canine mammary tumours (CMTs). The aims of this study were to assess the expression of MUC6 and Tn antigen in 55 benign and 77 malignant CMTs of different histological types and to investigate possible correlations with pathological features. MUC6 and Tn antigen were found to be significantly overexpressed in malignant compared with benign CMTs. MUC6 was significantly overexpressed in simple and complex carcinomas compared with simple and complex adenomas, respectively. When considering only the epithelial population, significant MUC6 overexpression was observed in carcinosarcomas when compared with benign mixed tumours. In addition, MUC6 was significantly overexpressed in simple compared with complex carcinomas. Finally, double-labelling immunofluorescence performed on seven malignant CMTs showed MUC6 and Tn co-expression. Therefore, MUC6 and Tn antigen overexpression is associated with malignant phenotypes of CMTs. PMID:22595635

Tavares, C; de Oliveira, J T; Lopes, C; Carvalheira, J; Matos, A J F; Rutteman, G; Reis, C A; Gärtner, F

2012-05-15

153

Expression of slow skeletal TnI in adult mouse hearts confers metabolic protection to ischemia  

PubMed Central

Changes in metabolic and myofilament phenotypes coincide in developing hearts. Posttranslational modification of sarcomere proteins influences contractility, affecting the energetic cost of contraction. However, metabolic adaptations to sarcomeric phenotypes are not well understood, particularly during pathophysiological stress. This study explored metabolic adaptations to expression of the fetal, slow skeletal muscle troponin I (ssTnI). Hearts expressing ssTnI exhibited no significant ATP loss during 5 minutes of global ischemia, while non-transgenic littermates (NTG) showed continual ATP loss. At 7 min ischemia TG-ssTnI hearts retained 80±12% of ATP vs. 49±6% in NTG (P<0.05). Hearts expressing ssTnI also had increased AMPK phosphorylation. The mechanism of ATP preservation was augmented glycolysis. Glycolytic end products (lactate and alanine) were 38% higher in TG-ssTnI than NTG at 2 min and 27% higher at 5 min. This additional glycolysis was supported exclusively by exogenous glucose, and not glycogen. Thus, expression of a fetal myofilament protein in adult mouse hearts induced elevated anaerobic ATP production during ischemia via metabolic adaptations consistent with the resistance to hypoxia of fetal hearts. The general findings hold important relevance to both our current understanding of the association between metabolic and contractile phenotypes and the potential for invoking cardioprotective mechanisms against ischemic stress.

Pound, Kayla M.; Arteaga, Grace M.; Fasano, Mathew; Wilder, Tanganyika; Fischer, Susan K.; Warren, Chad M.; Wende, Adam R.; Farjah, Mariam; Abel, E. Dale; Solaro, R. John; Lewandowski, E. Douglas

2011-01-01

154

40 CFR Appendix III to Part 1068 - High-Altitude Counties  

Code of Federal Regulations, 2010 CFR

...OF UTAH Beaver Box Elder Cache Carbon Daggett Davis Duchesne Emery Garfield Grand Iron Juab Kane Millard Morgan Piute Rich Salt Lake San Juan Sanpete Sevier Summit Tooele Uintah Utah Wasatch Wayne...

2010-07-01

155

High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn 5 Mob transposon  

Microsoft Academic Search

A DNA fragment of the broad host range plasmid RP4 carrying the cis-acting DNA recognition site for conjugative DNA transfer between bacterial cells (Mobsite) was cloned into the kanamycin-neomycin resistance transposon Tn5. Using conventrional transposon mutagenesis techniques the new transposon, called Tn5-Mob, can easily be inserted into the host DNA of gram-negative bacteria. A host replicon carrying Tn5-Mob is then

Reinhard Simon

1984-01-01

156

Construction of Tn5 lac, a Transposon That Fuses lacZ Expression to Exogenous Promoters, and Its Introduction into Myxococcus xanthus  

Microsoft Academic Search

A promoterless trp-lac fusion fragment was inserted near one end of the bacterial transposon Tn5 in the correct orientation to fuse lacZ gene expression to promoters outside Tn5. The resulting transposon, Tn5 lac, retains the kanamycin-resistance gene of Tn5 and transposes in Escherichia coli at 6% the frequency of Tn5 to many different sites in a bacteriophage lambda target. Expression

Lee Kroos; Dale Kaiser

1984-01-01

157

An Enterobacter plasmid as a new genetic background for the transposon Tn1331  

PubMed Central

Background Genus Enterobacter includes important opportunistic nosocomial pathogens that could infect complex wounds. The presence of antibiotic resistance genes in these microorganisms represents a challenging clinical problem in the treatment of these wounds. In the authors’ screening of antibiotic-resistant bacteria from complex wounds, an Enterobacter species was isolated that harbors antibiotic-resistant plasmids conferring resistance to Escherichia coli. The aim of this study was to identify the resistance genes carried by one of these plasmids. Methods The plasmids from the Enterobacter isolate were propagated in E. coli and one of the plasmids, designated as pR23, was sequenced by the Sanger method using fluorescent dyeterminator chemistry on a genetic analyzer. The assembled sequence was annotated by search of the GenBank database. Results Plasmid pR23 is composed of the transposon Tn1331 and a backbone plasmid that is identical to the plasmid pPIGDM1 from Enterobacter agglomerans. The multidrug-resistance transposon Tn1331, which confers resistance to aminoglycoside and beta lactam antibiotics, has been previously isolated only from Klebsiella. The Enterobacter plasmid pPIGDM1, which carries a ColE1-like origin of replication and has no apparent selective marker, appears to provide a backbone for propagation of Tn1331 in Enterobacter. The recognition sequence of Tn1331 transposase for insertion into pPIGDM1 is the pentanucleotide TATTA, which occurs only once throughout the length of this plasmid. Conclusion Transposition of Tn1331 into the Enterobacter plasmid pPIGDM1 enables this transposon to propagate in this Enterobacter. Since Tn1331 was previously isolated only from Klebsiella, this report suggests horizontal transfer of this transposon between the two bacterial genera.

Alavi, Mohammad R; Antonic, Vlado; Ravizee, Adrien; Weina, Peter J; Izadjoo, Mina; Stojadinovic, Alexander

2011-01-01

158

Taxonomy, purification and chemical characterization of four bioactive compounds from new Streptomyces sp. TN256 strain.  

PubMed

A new actinomycete strain designated TN256, producing antimicrobial activity against pathogenic bacteria and fungi, was isolated from a Tunisian Saharan soil. Morphological and chemical studies indicated that strain TN256 belonged to the genus Streptomyces. Analysis of the 16S rDNA sequence of strain TN256 showed a similarity level ranging between 99.79 and 97.8% within Streptomyces microflavus DSM 40331(T) and Streptomyces griseorubiginosus DSM 40469(T) respectively. The comparison of its physiological characteristics showed significant differences with the nearest species. Combined analysis of the 16 S rRNA gene sequences (FN687758), fatty acids profile, and results of physiological and biochemical tests indicated that there were genotypic and phenotypic differentiations of that isolate from other Streptomyces species neighbours. These date strongly suggest that strain TN256 represents a novel species with the type strain Streptomyces TN256 (=CTM50228(T)). Experimental validation by DNA-DNA hybridization would be required for conclusive confirmation. Four active products (1-4) were isolated from the culture broth of Streptomyces TN256 using various separation and purification steps and procedures. 1: N-[2-(1H-indol-3-yl)-2 oxo-ethyl] acetamide 'alkaloid' derivative; 2: di-(2-ethylhexyl) phthalate, a phthalate derivative; 3: 1-Nonadecene and 4: Cyclo (L: -Pro-L: -Tyr) a diketopiperazine 'DKP' derivative. The chemical structure of these four active compounds was established on the basis of spectroscopic studies NMR and by comparing with data from the literature. According to our biological studies, we showed in this work that the pure compounds (1-4) possess antibacterial and antifungal activities. PMID:22805798

Smaoui, S; Mathieu, F; Elleuch, L; Coppel, Y; Merlina, G; Karray-Rebai, I; Mellouli, L

2011-09-14

159

Novel Tn4371-ICE like element in Ralstonia pickettii and Genome mining for comparative elements  

PubMed Central

Background Integrative Conjugative Elements (ICEs) are important factors in the plasticity of microbial genomes. An element related to the ICE Tn4371 was discovered during a bioinformatic search of the Ralstonia pickettii 12J genome. This element was analysed and further searches carried out for additional elements. A PCR method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight Ralstonia pickettii and Ralstonia insidiosa strains were analysed for the presence of the element. Results Comparative sequence analysis of bacterial genomes has revealed the presence of a number of uncharacterised Tn4371-like ICEs in the genomes of several ? and ?- Proteobacteria. These elements vary in size, GC content, putative function and have a mosaic-like structure of plasmid- and phage-like sequences which is typical of Tn4371-like ICEs. These elements were found after a through search of the GenBank database. The elements, which are found in Ralstonia, Delftia, Acidovorax, Bordetella, Comamonas, Acidovorax, Congregibacter, Shewanella, Pseudomonas Stenotrophomonas, Thioalkalivibrio sp. HL-EbGR7, Polaromonas, Burkholderia and Diaphorobacter sp. share a common scaffold. A PCR method was designed (based on the Tn4371- like element detected in the Ralstonia pickettii 12J genome) to detect and characterise new elements of this type. Conclusion All elements found in this study possess a common scaffold of core genes but contain different accessory genes. A new uniform nomenclature is suggested for ICEs of the Tn4371 family. Two novel Tn4371-like ICE were discovered and characterised, using the novel PCR method described in two different isolates of Ralstonia pickettii from laboratory purified water.

2009-01-01

160

The global regulator H-NS acts directly on the transpososome to promote Tn10 transposition.  

PubMed

The histone-like nucleoid structuring (H-NS) protein is a global transcriptional regulator that is known to regulate stress response pathways and virulence genes in bacteria. It has also been implicated in the regulation of bacterial transposition systems, including Tn10. We demonstrate here that H-NS promotes Tn10 transposition by binding directly to the transposition complex (or transpososome). We present evidence that, upon binding, H-NS induces the unfolding of the Tn10 transpososome and helps to maintain the transpososome in an unfolded state. This ensures that intermolecular (as opposed to self-destructive intramolecular) transposition events are favored. We present evidence that H-NS binding to the flanking donor DNA of the transpososome is the initiating event in the unfolding process. We propose that by recruiting H-NS as a modulator of transposition, Tn10 has evolved a means of sensing changes in host physiology, as the amount of H-NS in the cell, as well its activity, are responsive to changes in environmental conditions. Sensing of environmental changes through H-NS would allow transposition to occur when it is most opportune for both the transposon and the host. PMID:16166383

Wardle, Simon J; O'Carroll, Michelle; Derbyshire, Keith M; Haniford, David B

2005-09-15

161

Identification of a second tetracycline-inducible polypeptide encoded by Tn10.  

PubMed Central

Three Tn10 polypeptides were detected by analyzing the proteins synthesized in ultraviolet light-irradiated Escherichia coli cells after infection with lambda::Tn10. One of these polypeptides was the previously identified 36,000-dalton TET polypeptide. The other two had approximate sizes of 25,000 and 13,000 daltons. The syntheses of both the TET polypeptide and the 25,000-dalton polypeptide were inducible by tetracycline in lambda-immune hosts. Similarly, the synthesis of the TET polypeptide was inducible in nonimmune hosts. However, the synthesis of the 25,000-dalton polypeptide was constitutive in nonimmune hosts. An amber mutation in a gene required for tetracycline resistance on lambda::Tn10 was isolated that eliminated the synthesis of the TET polypeptide in sup+ hosts but not the synthesis of the 25,000-dalton or the 13,000-dalton polypeptides. The expression of tetracycline resistance from wild-type Tn10 was found to be anomalous in E. coli strains carrying the amber suppressors supD, supE, and supF. In general, strains containing these nonsense suppressors were less resistant to tetracycline. Images

Zupancic, T J; King, S R; Pogue-Geile, K L; Jaskunas, S R

1980-01-01

162

Transposon Tn5 Excision in Yeast: Influence of DNA Polymerases alpha, delta, and ? and Repair Genes  

Microsoft Academic Search

Interaction between short repeats may be a source of genomic rearrangements and deletions. We investigated possible interactions between short (9 base pairs) direct repeats in yeast by using our previously described system for analyzing bacterial transposon Tn5 excision in yeast. Mutations of either POL3 or POL1, the proposed structural genes for polymerases delta and alpha, respectively, yield high levels of

D. A. Gordenin; A. L. Malkova; A. Peterzen; V. N. Kulikov; Y. I. Pavlov; E. Perkins; M. A. Resnick

1992-01-01

163

Lactobacillus plantarum TN8 exhibits protective effects on lipid, hepatic and renal profiles in obese rat.  

PubMed

This study aimed to first investigate the immuno-modulatory effects of six newly isolated lactic acid bacteria (LAB) on the peripheral blood mononuclear cells (PBMC) of Wistar rats. Except for Lactobacillus plantarum TN8, all the other strains were noted to induce high levels of pro-inflammatory cytokine IL-12 and low levels of anti-inflammatory cytokine IL-10. The strains also generated low ratios of IL-10/IL-12 cytokine. Strain TN8 was, on the other hand, noted to induce an increase in anti-inflammatory IL-10 cytokine secretion rates and a decrease in pro-inflammatory IL-12, IFN-? and TNF-? cytokine production. The oral administration of TN8 improved the hepatic and urinary functions of obese rats by inducing decreases (P < 0.05) in alanine amino transferase (ALAT), gamma glutamyl transferase (GGT), plasmatic triglycerides, total cholesterol concentrations, creatinine, urea, and body weight when compared to the control group of animals that underwent an increase in aspartate amino transferase (ASAT) and high density lipoprotein (HDL). Overall, the findings indicate that strain TN8 exhibited a number of attractive properties that might open new promising opportunities for the improvement of various parameters related to animal health performance and the avoidance of antibiotics and drugs as promoting factors. PMID:23891961

Ben Salah, Riadh; Trabelsi, Imen; Hamden, Khaled; Chouayekh, Hichem; Bejar, Samir

2013-07-26

164

Transposon mutagenesis of the anaerobic commensal, Bacteroides fragilis, using the EZ?TN5 transposome  

PubMed Central

Genetic analysis of Bacteroides fragilis (BF) is hindered due to the lack of efficient transposon mutagenesis methods. Here we describe a simple method for transposon mutagenesis using EZ?TN5, a commercially available system that we optimized for use in BF638R. The modified EZ?TN5 transposon contains an E. coli conditional origin of replication, a kanamycin resistance gene for E. coli, an erythromycin resistance gene for BF and 19 basepair transposase recognition sequences on either ends. Electroporation of the transposome (transposon-transposase complex) into BF638R yielded 3.2± 0.35×103 CFU/?g of transposon DNA. Modification of the transposon by the BF638R restriction/modification system increased transposition efficiency 6-fold. Electroporation of the EZ?TN5 transposome results in a single copy insertion of the transposon evenly distributed across the genome of BF638R and can be used to construct a BF638R transposon library. The transposon was also effective in mutating a BF clinical isolate and a strain of the related species, B. thetaiotaomicron. The EZ?TN5 based mutagenesis described here is more efficient than other transposon mutagenesis approaches previously reported for BF.

Veeranagouda, Yaligara; Husain, Fasahath; Wexler, Hannah M.

2012-01-01

165

75 FR 28302 - American Food and Vending Spring Hill, TN; Notice of Negative Determination Regarding Application...  

Federal Register 2010, 2011, 2012, 2013

...TA-W-72,606] American Food and Vending Spring Hill, TN; Notice of Negative Determination...workers at American Food and Vending, Spring Hill, Tennessee, was based on the findings...provided services to General Motors, Spring Hill, Tennessee, and were laid off...

2010-05-20

166

Tn7-Based Genome-Wide Random Insertional Mutagenesis of Candida glabrata  

PubMed Central

We describe and characterize a method for insertional mutagenesis of the yeast pathogen Candida glabrata using the bacterial transposon Tn7. Tn7 was used to mutagenize a C. glabrata genomic fosmid library. Pools of random Tn7 insertions in individual fosmids were recovered by transformation into Escherichia coli. Subsequently, these were introduced by recombination into the C. glabrata genome. We found that C. glabrata genomic fragments carrying a Tn7 insertion could integrate into the genome by nonhomologous recombination, by single crossover (generating a duplication of the insertionally mutagenized locus), and by double crossover, yielding an allele replacement. We were able to generate a highly representative set of ?104 allele replacements in C. glabrata, and an initial characterization of these shows that a wide diversity of genes were targeted in the mutagenesis. Because the identity of disrupted genes for any mutant of interest can be rapidly identified, this method should be of general utility in functional genomic characterization of this important yeast pathogen. In addition, the method might be broadly applicable to mutational analysis of other organisms.

Castano, Irene; Kaur, Rupinder; Pan, Shihjung; Cregg, Robert; Penas, Alejandro De Las; Guo, Nini; Biery, Matthew C.; Craig, Nancy L.; Cormack, Brendan P.

2003-01-01

167

The low TN:TP ratio, a cause or a result of Microcystis blooms?  

Microsoft Academic Search

An enclosure experiment in the shallow, subtropical Lake Donghu, China, was performed in the summer of 2001 to examine the effect of TN:TP (total phosphorus) ratios and P-reduction on the occurrence of Microcysitis blooms. The treatments were performed with enough amounts of N but with different amounts of P in the water column and sediment. Microcystis blooms occurred in the

Liqiang Xie; Ping Xie; Sixin Li; Huijuan Tang; Hong Liu

2003-01-01

168

77 FR 12759 - Proposed Amendment of Class E Airspace; Tullahoma, TN  

Federal Register 2010, 2011, 2012, 2013

...Arnold Air Force Base has been closed and therefore controlled airspace...triplicate to the Docket Management System (see ADDRESSES section for...Proposed Rulemaking distribution System, which describes the application...more above the surface of the earth. * * * * * ASO TN E5...

2012-03-02

169

The global regulator H-NS acts directly on the transpososome to promote Tn10 transposition  

PubMed Central

The histone-like nucleoid structuring (H-NS) protein is a global transcriptional regulator that is known to regulate stress response pathways and virulence genes in bacteria. It has also been implicated in the regulation of bacterial transposition systems, including Tn10. We demonstrate here that H-NS promotes Tn10 transposition by binding directly to the transposition complex (or transpososome). We present evidence that, upon binding, H-NS induces the unfolding of the Tn10 transpososome and helps to maintain the transpososome in an unfolded state. This ensures that intermolecular (as opposed to self-destructive intramolecular) transposition events are favored. We present evidence that H-NS binding to the flanking donor DNA of the transpososome is the initiating event in the unfolding process. We propose that by recruiting H-NS as a modulator of transposition, Tn10 has evolved a means of sensing changes in host physiology, as the amount of H-NS in the cell, as well its activity, are responsive to changes in environmental conditions. Sensing of environmental changes through H-NS would allow transposition to occur when it is most opportune for both the transposon and the host.

Wardle, Simon J.; O'Carroll, Michelle; Derbyshire, Keith M.; Haniford, David B.

2005-01-01

170

Tn 916 -induced mutants of Clostridium acetobutylicum defective in regulation of solvent formation  

Microsoft Academic Search

Sixteen Tn916-induced mutants of Clostridium acetobutylicum were selected that were defective in the production of acetone and butanol. Formation of ethanol, however, was only partially affected. The strains differed with respect to the degree of solvent formation ability and could be assigned to three different groups. Type I mutants (2 strains) were completely defective in acetone and butanol production and

Joachim Bertram; Anita Kuhn; Peter Diirre

1990-01-01

171

A high-throughput assay for Tn5 Tnp-induced DNA cleavage  

PubMed Central

Transposition causes genomic instability by mobilizing DNA elements. This phenomenon is mechanistically related to other DNA rearrangements, such as V(D)J recombination and retroviral DNA integration. A conserved active site architecture within the transposase/integrase superfamily catalyzes these distinct phenomena. The Tn5 transposase (Tnp) falls within this protein class, and many intermediates of the Tn5 transposition reaction have been characterized. Here, we describe a method for the rapid identification of Tn5 Tnp small molecule effectors. This high-throughput screening strategy will aid in the identification of compounds that perturb Tnp-induced DNA cleavage. This method is advantageous, since it identifies effectors that specifically inhibit catalysis without inhibiting Tnp–DNA binding interactions. Effectors identified using this method will serve as a valuable aid both in the isolation and characterization of metal-bound reaction intermediates and in co-crystallization studies involving the effector, Tnp and DNA, to identify the structural basis of the interaction. Furthermore, since Tn5 Tnp shares a similar active site architecture to other transposase/integrase superfamily members, this strategy and any effectors identified using this method will be readily applicable to these other systems.

Ason, Brandon; Reznikoff, William S.

2004-01-01

172

Targeting Tn5 Transposase Identifies Human Immunodeficiency Virus Type 1 Inhibitors  

PubMed Central

Human immunodeficiency virus (HIV) type 1 (HIV-1) integrase is an underutilized drug target for the treatment of HIV infection. One limiting factor is the lack of costructural data for use in the rational design or modification of integrase inhibitors. Tn5 transposase is a structurally well characterized, related protein that may serve as a useful surrogate. However, little data exist on inhibitor cross-reactivity. Here we screened 16,000 compounds using Tn5 transposase as the target and identified 20 compounds that appear to specifically inhibit complex assembly. Six were found to also inhibit HIV-1 integrase. These compounds likely interact with a highly conserved region presumably within the catalytic core. Most promising, several cinnamoyl derivatives were found to inhibit HIV transduction in cells. The identification of integrase inhibitors from a screen using Tn5 transposase as the target illustrates the utility of Tn5 as a surrogate for HIV-1 integration even though the relationship between the two systems is limited to the active site architecture and catalytic mechanism.

Ason, Brandon; Knauss, Daniel J.; Balke, Allison M.; Merkel, George; Skalka, Anna Marie; Reznikoff, William S.

2005-01-01

173

Genome-Scale Identification of Resistance Functions in Pseudomonas aeruginosa Using Tn-seq  

PubMed Central

We describe a deep-sequencing procedure for tracking large numbers of transposon mutants of Pseudomonas aeruginosa. The procedure employs a new Tn-seq methodology based on the generation and amplification of single-strand circles carrying transposon junction sequences (the Tn-seq circle method), a method which can be used with virtually any transposon. The procedure reliably identified more than 100,000 transposon insertions in a single experiment, providing near-saturation coverage of the genome. To test the effectiveness of the procedure for mutant identification, we screened for mutations reducing intrinsic resistance to the aminoglycoside antibiotic tobramycin. Intrinsic tobramycin resistance had been previously analyzed at genome scale using mutant-by-mutant screening and thus provided a benchmark for evaluating the new method. The new Tn-seq procedure identified 117 tobramycin resistance genes, the majority of which were then verified with individual mutants. The group of genes with the strongest mutant phenotypes included nearly all (13 of 14) of those with strong mutant phenotypes identified in the previous screening, as well as a nearly equal number of new genes. The results thus show the effectiveness of the Tn-seq method in defining the genetic basis of a complex resistance trait of P. aeruginosa and indicate that it can be used to analyze a variety of growth-related processes.

Gallagher, Larry A.; Shendure, Jay; Manoil, Colin

2011-01-01

174

Luisa Valenzuela at Elvis Presley’s house in Memphis, TN, April 1988  

Microsoft Academic Search

Luisa Valenzuela at Elvis Presley’s house in Memphis, TN, April 1988. Valenzuela is an Argentinian novelist and short story writer celebrated for her avant-garde style and feminist critique of social hierarchy and Argentinian dictatorship. Photographer: Fernando Burgos. Related article: Issue 28, En Memphis con Luisa Valenzuela: Voces y Viajes, an interview with Valenzuela by Fernando Burgos and M. J. Fenwick,

Fernando Burgos

1988-01-01

175

Elution of Nitrate at the NABIR Field Research Center, Oak Ridge Reservation, Oak Ridge, TN  

Microsoft Academic Search

As part of a bioremediation project for the in situ bioreduction of uranium at the Department of Energy Natural and Accelerated Bioremediation Research (NABIR) Field Research Center (FRC) in Oak Ridge, TN, aquifer and groundwater conditioning is required before conducting the remediation experiment. One step includes flushing of the aquifer with pH-adjusted fresh water in order to remove extremely high

M. N. Fienen; C. S. Criddle; P. M. Jardine; P. K. Kitanidis; T. L. Mehlhorn; D. B. Watson; W. Wu

2003-01-01

176

The global bacterial regulator H-NS promotes transpososome formation and transposition in the Tn5 system.  

PubMed

The histone-like nucleoid structuring protein (H-NS) is an important regulator of stress response and virulence genes in gram-negative bacteria. In addition to binding regulatory regions of genes in a structure-specific manner, H-NS also binds in a structure-specific manner to sites in the Tn10 transpososome, allowing it to act as a positive regulator of Tn10 transposition. This is the only example to date of H-NS regulating a transposition system by interacting directly with the transposition machinery. In general, transposition complexes tend to include segments of deformed DNA and given the capacity of H-NS to bind such structures, and the results from the Tn10 system, we asked if H-NS might regulate another transposition system (Tn5) by directly binding the transposition machinery. We show in the current work that H-NS does bind Tn5 transposition complexes and use hydroxyl radical footprinting to characterize the H-NS interaction with the Tn5 transpososome. We also show that H-NS can promote Tn5 transpososome formation in vitro, which correlates with the Tn5 system showing a dependence on H-NS for transposition in vivo. Taken together the results suggest that H-NS might play an important role in the regulation of many different bacterial transposition systems and thereby contribute directly to lateral gene transfer. PMID:19042975

Whitfield, Crystal R; Wardle, Simon J; Haniford, David B

2008-11-28

177

Techniques for Multistage A2 Lattice Structure Construction and Calculation Using Triangular Number (Tn) and Square Number (Sn)  

Microsoft Academic Search

In this paper, the connection between triangular number (Tn), square number (Sn) for construction and counting the A2 lattice number in lattice vector quantization is presented. We also observe that unique hexagonal A2 lattice can be formed from two triangular numbers (Tn) and a square number (Sn) combination. Furthermore, the number of multistage A2 lattice (Wn) is derived from A2

I. Syafalni; M. F. M. Salleh

2010-01-01

178

Josephine County Preliminary Comprehensive Plan.  

National Technical Information Service (NTIS)

Contents: General characteristics; Forest lands; Cleared land; Planning area analysis; Forecast of employment for Josephine County (1985); Forecasts of population for Josephine County (1985); Distribution of employment within the County (1985); Distributi...

1969-01-01

179

Two-stage soil infiltration treatment system for treating ammonium wastewaters of low COD/TN ratios.  

PubMed

Soil infiltration treatment (SIT) is ineffective to treat ammonium wastewaters of total nitrogen (TN) > 100 mg l(-1). This study applied a novel two-stage SIT process for effective TN removal from wastewaters of TN>100 mg l(-1) and of chemical oxygen demand (COD)/TN ratio of 3.2-8.6. The wastewater was first fed into the soil column (stage 1) at hydraulic loading rate (HLR) of 0.06 m(3) m(-2) d(-1) for COD removal and total phosphorus (TP) immobilization. Then the effluent from stage 1 was fed individually into four soil columns (stage 2) at 0.02 m(3) m(-2) d(-1) of HLR with different proportions of raw wastewater as additional carbon source. Over the one-year field test, balanced nitrification and denitrification in the two-stage SIT revealed excellent TN removal (>90%) from the tested wastewaters. PMID:23186658

Lei, Zhongfang; Wu, Ting; Zhang, Yi; Liu, Xiang; Wan, Chunli; Lee, Duu-Jong; Tay, Joo-Hwa

2012-11-02

180

Mono County update  

Microsoft Academic Search

On February 9, 1988, the Mono County Board of Supervisors voted to approve Bonneville Pacific Corporation's Mammoth Chance Geothermal Project. The project is an air-cooled, binary, geothermal power plant, 10 megawatts, net. The Mono County Board of Supervisors issued a project use-permit with vigorous and stringent conditions. Specific emphasis was placed on the establishment of a monitoring program designed to

Lyster

1987-01-01

181

Penobscot County Health Inquiry.  

National Technical Information Service (NTIS)

A community profile is presented for Penobscot County, Maine. The county is the fourth largest in Maine and is part of the service area of the Northeast Health Planning Council. The profile opens with a description of the geographic characteristics of the...

S. Fulton T. Ramsey J. M. Cauble

1974-01-01

182

Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha.  

PubMed Central

Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the inability to grow with reduced sulfur compounds (Sox-). Four classes of Sox- mutants were obtained. Three were of different pleiotropic phenotypes: (i) unable to grow with formate, nitrate, and xanthine; (this class strongly suggested the involvement of a molybdenum cofactor in inorganic sulfur-oxidizing ability); (ii) no growth with hydrogen; (iii) slight growth with hydrogen and formate. Two plasmids, pHG41 (about 450 kilobase pairs) and pHG42 (110 kilobases), were identified in lysates of T. pantotropha. In one Sox- mutant pHG41 could not be detected. Revertant analysis suggested that pHG41 and pHG42 were not involved in the Sox character. Images

Chandra, T S; Friedrich, C G

1986-01-01

183

Characterization of Tn917 insertion mutants of Staphylococcus epidermidis affected in biofilm formation.  

PubMed Central

Biofilm formation is thought to result from the concerted action of primary attachment to a specific surface and accumulation in multilayered cell clusters. Here we describe the isolation and characterization of transposon (Tn917) mutants of Staphylococcus epidermidis O-47 which were biofilm negative in the polystyrene microtiter plate assay. Among 5,000 Tn917 insertion mutants, 4 biofilm-negative mutants were isolated. Each mutant carried one copy of Tn917. The mutants were divided into two phenotypic classes: class A (mut1 and mut1a) and class B (mut2 and mut2a). Mutants of phenotypic class A lacked four cell surface proteins, were less hydrophobic, and were affected in primary attachment to polystyrene, but were still able to form multilayered cell clusters. They were able to form a biofilm on a glass surface, a trait that was even more pronounced than in the wild-type stain O-47. Loss of several surface proteins might have led to the reduced surface hydrophilic structures, thus favoring primary attachment to a glass surface and leading to subsequent biofilm formation. Mutants of phenotype class B were able to attach to polystyrene but were unable to form multilayered cell clusters, had unchanged cell surface proteins and hydrophobicity, and were unable to form a biofilm on a glass surface, mut1 and mut2 could be complemented by wild-type DNA fragments containing the Tn917 insertion sites of mut1 and mut2, respectively. The complemented biofilm-positive clone mut1 (pRC20) produced a 60-kDa protein which is postulated to function as the adhesin for binding to plastic. The traits of binding to polystyrene and the ability to form multilayered cell clusters are phenotypically and genetically distinct.

Heilmann, C; Gerke, C; Perdreau-Remington, F; Gotz, F

1996-01-01

184

Five naturally bioactive molecules including two rhamnopyranoside derivatives isolated from the Streptomyces sp. strain TN58  

Microsoft Academic Search

Extraction of 25 L fermentation broth of the newly isolated Streptomyces sp. strain TN58 and various separation and purification steps led to the isolation of five bioactive metabolites, namely brevianamide F (C1), reported from a streptomycete for the first time, N -acetyltryptamine (C2), thiazolidomycin (C3), and two rhamnopyranosides (C4 and C5). These two rhamnopyranosides were produced directly, without precursor addition.

Raoudha Ben Ameur Mehdi; Khaled A. Shaaban; Ines Karray Rebai; Slim Smaoui; Samir Bejar; Lotfi Mellouli

2009-01-01

185

Analysis of Tn 916 -induced mutants of Clostridium acetobutylicum altered in solventogenesis and sporulation  

Microsoft Academic Search

Summary The conjugative transposon Tn916 was used for mutagenesis ofClostridium acetobutylicum ATCC 824. Tetracycline-resistant mutants were screened for loss of granulose synthesis and five classes of granulose mutants, that contained single transposon insertions, were identified on the basis of altered solvent production. Class 1 mutants did not make acetone or butanol, lacked activity of enzymes induced during solventogenesis, and did

Donald M. Mattsson; Palmer Rogers

1994-01-01

186

Persistent repression of a functional allele can be responsible for galactosyltransferase deficiency in Tn syndrome.  

PubMed Central

A human hematopoietic disorder designated as Tn syndrome or permanent mixed-field polyagglutinability has been ascribed to a stem cell mutation leading to a specific deficiency of UDP-Gal:GalNAc alpha 1-O-Ser/Thr beta 1-3 galactosyltransferase (beta 3 Gal-T) activity in affected cells. To test for the possibility that an allele of the beta 3Gal-T gene might be repressed instead of mutated, we have investigated whether 5-azacytidine or sodium n-butyrate, both inducers of gene expression, would reactivate expression of beta 3Gal-T in cloned enzyme-deficient T cells derived from a patient affected by the Tn syndrome. Flow cytometry revealed that a single treatment induced de novo expression of the Thomsen-Friedenreich antigen (Gal beta 1-3GalNAc-R), the product of beta 3Gal-T activity. In addition, a sialylated epitope on CD43 (leukosialin), which is present on normal but not on beta 3Gal-T-deficient T cells, was also reexpressed. Although no beta 3Gal-T activity was detectable in untreated Tn syndrome T cells, after exposure to 5-azaC,beta 3Gal-T activity reached nearly normal values. Both agents failed to reactivate beta 3Gal-T in Jurkat T leukemic cells, which also lack beta 3Gal-T activity. These data demonstrate that Tn syndrome T cells contain an intact beta 3Gal-T gene copy and that the enzyme deficiency in this patient is due to a persistent and complete but reversible repression of a functional allele. In contrast, the cause of beta 3Gal-T deficiency appears to be different in Jurkat T cells. Images

Thurnher, M; Rusconi, S; Berger, E G

1993-01-01

187

The transposon Tn 1 as a probe for studying ColE1 structure and function  

Microsoft Academic Search

Insertion of the transposable genetic element Tn1 into different sites of plasmid ColE1 results in a number of mutant phenotypes. Whereas all plasmids examined were present in normal amount, all showed reduced immunity to killing by colicin E1. Of six insertions isolated after conjugation, five fail to produce colicin, are conjugally proficient (transmissible), and map within a 500 nucleotide region

Gordon Dougan; David Sherratt

1977-01-01

188

Bioactive Secondary Metabolites from a New Terrestrial Streptomyces sp. TN262  

Microsoft Academic Search

During our search for Streptomyces spp. as new producers of bioactive secondary metabolites, the ethyl acetate extract of the new terrestrial Streptomyces isolate TN262 delivered eight antimicrobially active compounds. They were identified as 1-acetyl-?-carboline (1), tryptophol (2), cineromycin B (3), 2,3-dihydrocineromycin B (4), cyclo-(tyrosylprolyl) (5), 3-(hydroxyacetyl)-indole (6), brevianamide F (7), and cis-cyclo-(l-prolyl-l-leucyl) (8). Three further metabolites were detected in the

Lobna Elleuch; Mohamed Shaaban; Slim Smaoui; Lotfi Mellouli; Ines Karray-Rebai; Lilia Fourati-Ben Fguira; Khaled A. Shaaban; Hartmut Laatsch

2010-01-01

189

A Tn7-like transposon is present in the glmUS region of the obligately chemoautolithotrophic bacterium Thiobacillus ferrooxidans.  

PubMed

The region downstream of the Thiobacillus ferrooxidans ATCC 33020 atp operon was examined, and the genes encoding N-acetylglucosamine-1-uridyltransferase (glmU) and glucosamine synthetase (glmS) were found. This atpEFHAGDC-glmUS gene order is identical to that of Escherichia coli. The T. ferrooxidans glmS gene was shown to complement E. coli glmS mutants for growth on minimal medium lacking glucosamine. A Tn7-like transposon, Tn5468, was found inserted into the region immediately downstream of the glmS gene in a manner similar to the site-specific insertion of transposon Tn7 within the termination region of the E. coli glmS gene. Tn5468 was sequenced, and Tn7-like terminal repeat sequences as well as several open reading frames which are related to the Tn7 transposition genes tnsA, tnsB, tnsC, and tnsD were found. Tn5468 is the closest relative of Tn7 to have been characterized to date. Southern blot hybridization indicated that a similar or identical transposon was present in three T. ferrooxidans strains isolated from different parts of the world but not in two Thiobacillus thiooxidans strains or a Leptospirillum ferrooxidans strain. Since T. ferrooxidans is an obligately acidophilic autotroph and E. coli is a heterotroph, ancestors of the Tn7-like transposons must have been active in a variety of physiologically different bacteria so that their descendants are now found in bacteria that occupy very different ecological niches. PMID:9603897

Oppon, J C; Sarnovsky, R J; Craig, N L; Rawlings, D E

1998-06-01

190

Alteration of surface properties in a Tn5 mutant strain of Rhizobium trifolii 0403.  

PubMed

A symbiotically defective mutant strain of Rhizobium trifolii, UR251, was obtained by transposon Tn5 mutagenesis of R. trifolii 0403 rif and recognized by its partially ineffective (Fix +/-) phenotype on white clover plants. UR251 had a single Tn5 insertion in plasmid DNA, a wild-type plasmid pattern, and no detectable Mu DNA sequences originally present in the vector used for Tn5 mutagenesis. Agglutination by the clover lectin trifoliin A and attachment to clover root hairs was higher with UR251 than with the wild-type strain. The capsular polysaccharide (CPS) of UR251 was altered, as shown by a slower rate of CPS depolymerization with a CPS beta-lyase, PD-I; more pyruvate and less acetate and 3-hydroxybutanoate noncarbohydrate substitutions as quantitated by 1H nuclear magnetic resonance; and a higher pyruvyl transferase activity (enzymatic pyruvylation of lipid-bound saccharides). The site of increased pyruvylation in the CPS of UR251 was on the terminal galactose of the branch of the repeating oligosaccharide unit. These results show that the level of noncarbohydrate substitutions of the CPS as well as pyruvyl transferase activity are altered in R. trifolii UR251 and that trifoliin A-binding ability and clover root hair attachment are improved in this mutant strain of R. trifolii 0403 rif. PMID:3029022

Gardiol, A E; Hollingsworth, R I; Dazzo, F B

1987-03-01

191

Alteration of surface properties in a Tn5 mutant strain of Rhizobium trifolii 0403.  

PubMed Central

A symbiotically defective mutant strain of Rhizobium trifolii, UR251, was obtained by transposon Tn5 mutagenesis of R. trifolii 0403 rif and recognized by its partially ineffective (Fix +/-) phenotype on white clover plants. UR251 had a single Tn5 insertion in plasmid DNA, a wild-type plasmid pattern, and no detectable Mu DNA sequences originally present in the vector used for Tn5 mutagenesis. Agglutination by the clover lectin trifoliin A and attachment to clover root hairs was higher with UR251 than with the wild-type strain. The capsular polysaccharide (CPS) of UR251 was altered, as shown by a slower rate of CPS depolymerization with a CPS beta-lyase, PD-I; more pyruvate and less acetate and 3-hydroxybutanoate noncarbohydrate substitutions as quantitated by 1H nuclear magnetic resonance; and a higher pyruvyl transferase activity (enzymatic pyruvylation of lipid-bound saccharides). The site of increased pyruvylation in the CPS of UR251 was on the terminal galactose of the branch of the repeating oligosaccharide unit. These results show that the level of noncarbohydrate substitutions of the CPS as well as pyruvyl transferase activity are altered in R. trifolii UR251 and that trifoliin A-binding ability and clover root hair attachment are improved in this mutant strain of R. trifolii 0403 rif. Images

Gardiol, A E; Hollingsworth, R I; Dazzo, F B

1987-01-01

192

Anomalies of the anaerobic tricarboxylic acid cycle in Shewanella oneidensis revealed by Tn-seq.  

PubMed

The availability of increasingly inexpensive sequencing combined with an ever-expanding molecular biology toolbox has transported classical bacterial genetics into the 21st century. Whole genome genetic fitness analysis using transposon mutagenesis combined with next-generation high-throughput sequencing (Tn-seq) promises to revolutionize systems level analysis of microbial metabolism. Tn-seq measures the frequency of actual members of a heterogeneous mutant pool undergoing purifying selection to determine the contribution of every non-essential gene in the genome to the fitness of an organism under a given condition. Here we use Tn-seq to assess gene function in the Gram negative ?-proteobacterium Shewanella oneidensis strain MR-1. In addition to being a model environmental organism, there is considerable interest in using S.?oneidensis as a platform organism for bioremediation and biotechnology, necessitating a complete understanding of the metabolic pathways that may be utilized. Our analysis reveals unique aspects of S.?oneidensis metabolism overlooked by over 30 years of classical genetic and systems level analysis. We report the utilization of an alternative citrate synthase and describe a dynamic branching of the S.?oneidensis anaerobic tricarboxylic acid cycle, unreported in any other organism, which may be a widespread strategy for microbes adept at dissipating reducing equivalents via anaerobic respiration. PMID:22925268

Brutinel, Evan D; Gralnick, Jeffrey A

2012-08-27

193

IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration  

PubMed Central

The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This strand transfer transpososome (STT) does appear to support an intermolecular disintegration reaction, but only at a very low level. Strikingly, assembly of the STT is not dependent on IHF, a host protein that is required for de novo assembly of all previously characterized Tn10 transpososomes. We suggest that disintegration substrates are able to form both transposon end and target type contacts with transposase because of their enhanced conformational flexibility. This probably allows the conformation of DNA within the complex that prevents the destructive disintegration reaction, and is responsible for relaxing the DNA sequence requirements for STT formation relative to other Tn10 transpososomes.

Stewart, Barry J.; Wardle, Simon J.; Haniford, David B.

2002-01-01

194

Tn10 insertions in the pfkB region of Escherichia coli.  

PubMed

The locus pfkB is known to determine expression of a minor phosphofructokinase (Pfk-2). Pfk-2 and pfkB seem to be dispensable, since Tn10 insertions in pfkB, as well as deletions from Tn10 nearby, are obtainable. Strains deleted for both pfkA and pgkB are unable to grow at all on sugars whose primary route of metabolism is via fructose 6-phosphate, confirming earlier reports implicating the low Pfk-2 activity, rather than the pentose-phosphate pathway, as needed for the slow growth on sugars of pfkA pfkB+ strains. The pfkB locus probably contains the structural gene for Pfk-2, since a mutation closely linked to pfkB1, which affects growth on glycerol, is found to alter the enzyme. Partial phenotypic suppression of the pfkA mutant phenotype results from Tn10 insertion very close to the pps gene, ca. 0.5 min from pgkB. The insertion does not clearly affect either Pfk-2 or phosphoenolpyruvate synthetase, and the mechanism of suppression is unclear. PMID:6268614

Daldal, F; Fraenkel, D G

1981-09-01

195

Incised valley fill interpretation for Mississippian Black Hand Sandstone, Appalachian Basin, USA: Implications for glacial eustasy at Kinderhookian Osagean (Tn2 Tn3) boundary  

NASA Astrophysics Data System (ADS)

Lower Mississippian strata of east-central Ohio are predominantly fine-grained marine deposits of the Cuyahoga and Logan formations. Within these sediments is the Black Hand Sandstone of the Cuyahoga Formation. The Black Hand Sandstone is a multistory, crossbedded, coarse-grained conglomeratic sandstone. The contact between the Black Hand Sandstone and the subjacent Cuyahoga Formation is sharp and scoured, with intraclasts of the Cuyahoga Formation incorporated into the basal Black Hand Sandstone. The Black Hand Sandstone was previously thought to represent a distributary channel deposit; however, the combination of lithofacies and architectural elements indicates deposition in a braided stream setting. The Cuyahoga Formation was deposited in a shallow marine setting. The erosional basal contact of the Black Hand Sandstone and the juxtaposition of fluvial and marine sediments suggests a sequence boundary. The geographic distribution of the Black Hand Sandstone combined with the evidence for a sequence boundary suggests deposition in an incised valley. The age of the Black Hand Sandstone is key to inferring the causes of valley incision. The Black Hand Sandstone is nearly devoid of body fossils, necessitating a biostratigraphic analysis of the surrounding Cuyahoga and Logan formations. Analysis indicates the Logan Formation is early Osagean age. Data from the Cuyahoga Formation suggest a Kinderhookian age with a possible transition to the Osagean in the uppermost Cuyahoga Formation. This constrains the age of the Black Hand Sandstone to the transition at the Kinderhookian Osagean boundary. Recent reports indicate late Kinderhookian (Tournaisian, Tn2) Gondwanan glaciation based upon tillites and sharp excursions in stable-isotope curves. A glacio-eustatic fall in sea level is inferred to have caused incision of the Cuyahoga Formation, followed by deposition of the Black Hand Sandstone and Logan Formation during the subsequent sea level rise. The associated unconformity correlates to the sequence boundary at the Kinderhookian Osagean boundary in the stratotype area of North America, and the correlative Tn2 Tn3 boundary worldwide, supporting the hypothesis of a global eustatic event at this time.

Matchen, David L.; Kammer, Thomas W.

2006-09-01

196

County Amenities and Net Migration  

Microsoft Academic Search

U.S. county-level net migration data and a general spatial model are used to examine the effects of various amenities on migration decisions. Results suggest that higher county cancer risks and the presence of superfund sites in a county, or a higher ranking on the Environmental Protection Agency's hazard ranking system, reduce the relative attractiveness of a county to prospective migrants,

Anil Rupasingha; Stephan J. Goetz

2004-01-01

197

TN method for the critical thickness of one-speed neutrons in a slab with forward and backward scattering  

NASA Astrophysics Data System (ADS)

The critical slab problem in the case of combination of forward and backward scattering with usual isotropic scattering is studied for one-speed neutrons in a uniform finite slab by using TN method based on Chebyshev polynomial approximation and Marshak boundary conditions. It is shown that TN method gives accurate results in one-dimensional geometry and is very efficient both in derivation of equations and rapid convergence. Numerical results obtained by TN method are compared against the PN method in tabular form, which agreed quite well.

Öztürk, Hakan; Anli, Fikret; Güngör, Süleyman

2007-06-01

198

Mutational analysis of the Escherichia coli glpFK region with Tn5 mutagenesis and the polymerase chain reaction.  

PubMed Central

Transposon Tn5 mutagenesis of the Escherichia coli chromosome was used to isolate 21 independent insertion mutations conferring an altered colony color phenotype on MacConkey-glycerol plates. The polymerase chain reaction was used to map 16 of these Tn5 insertions within the glpFK region at 88 min. The most polar Tn5 insertion was shown by nucleotide sequencing to be in the proposed glpF open reading frame. The data suggest that the glpF and glpK genes are in an operon with a bent DNA segment (BENT-6) involved in transcriptional regulation of this operon. Images

Lupski, J R; Zhang, Y H; Rieger, M; Minter, M; Hsu, B; Ooi, B G; Koeuth, T; McCabe, E R

1990-01-01

199

Simple mucin-type carbohydrate antigens (Tn, sialosyl-Tn, T and sialosyl-T) and gp 230 mucin-like glycoprotein are candidate markers for neoplastic transformation of the human cervix  

Microsoft Academic Search

Mucins and simple mucin-type carbohydrates are cancer-associated antigens in several human tumors. Expression of Tn, sialosyl-Tn,\\u000a Thomsen-Friedenreich (T), sialosyl-T and of a recently identified mucin-like glycoprotein (gp230) has not yet been thoroughly\\u000a investigated in human cervix carcinogenesis. In the present study sections from normal cervix (n=10), CIN III lesions (n=10), and invasive carcinomas (n=47) were evaluated immunohistochemically using monoclonal antibodies.

Carla Carrilho; Martha Cantel; Patricia Gouveia; Leonor David

2000-01-01

200

76 FR 60802 - Foreign-Trade Zone 77-Memphis, TN; Withdrawal of Request for Temporary/Interim Manufacturing...  

Federal Register 2010, 2011, 2012, 2013

...Withdrawal of Request for Temporary/Interim Manufacturing Authority; Flextronics Logistics USA, Inc., (Cell Phone/Mobile Handset Kitting). Memphis, TN Notice is hereby given of the withdrawal of the application of the City of...

2011-09-30

201

76 FR 34799 - Permanent Dam Safety Modification at Cherokee, Fort Loudoun, Tellico, and Watts Bar Dams, TN  

Federal Register 2010, 2011, 2012, 2013

...TENNESSEE VALLEY AUTHORITY Permanent Dam Safety Modification at Cherokee, Fort Loudoun, Tellico, and Watts Bar Dams, TN AGENCY: Tennessee Valley Authority...permanent modifications to the existing dam facilities at Cherokee, Fort...

2011-06-14

202

75 FR 6636 - Foreign-Trade Zone 77-Memphis, TN Application for Subzone Cummins, Inc. (Engine Components...  

Federal Register 2010, 2011, 2012, 2013

...DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 8-2010] Foreign-Trade Zone 77--Memphis, TN Application for...An application has been submitted to the Foreign-Trade Zones Board (the Board) by the City...

2010-02-10

203

10. Photocopy of photograph showing the three Walker sisters ginning ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Photocopy of photograph showing the three Walker sisters ginning cotton. Misses Hettie, Martha and Louisa are from left to right. The original photograph was taken on May 21, 1936 by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is III-A-HSE-9642. - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

204

5. Photocopy of May 21, 1936 photograph showing the interior ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Photocopy of May 21, 1936 photograph showing the interior of the Big House (main portion, living-bedroom). The woman is Hettie Walker. The fireplace is on the house's front side. The original photograph was taken by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8086. - Walker Family Farm, Big House, Gatlinburg, Sevier County, TN

205

The global bacterial regulator H-NS promotes transpososome formation and transposition in the Tn5 system  

Microsoft Academic Search

The histone-like nucleoid structuring protein (H-NS) is an important regulator of stress response and virulence genes in gram-negative bacteria. In addition to binding regulatory regions of genes in a structure-specific manner, H-NS also binds in a structure-specific manner to sites in the Tn10 trans- pososome, allowing it to act as a positive regulator of Tn10 transposition. This is the only

Crystal R. Whitfield; Simon J. Wardle; David B. Haniford

2008-01-01

206

The Global Bacterial Regulator H-NS Promotes Transpososome Formation and Transposition in the Tn5 System  

Microsoft Academic Search

The histone-like nucleoid structuring protein (H-NS) is an important regulator of stress response and virulence genes in gram-negative bacteria. In addition to binding regulatory regions of genes in a structure-specific manner, H-NS also binds in a structure-specific manner to sites in the Tn10 transpososome, allowing it to act as a positive regulator of Tn10 transposition. This is the only example

Crystal R. Whitfield; Simon J. Wardle; David B. Haniford

2009-01-01

207

Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant  

Microsoft Academic Search

Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain\\u000a PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was\\u000a to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5™Tnp Transposome™ (Epicentre).\\u000a Up

Luc F. M. Rouws; Jean L. Simões-Araújo; Adriana S. Hemerly; José I. Baldani

2008-01-01

208

Phenotypic Screening of Escherichia coli K-12 Tn5 Insertion Libraries, Using Whole-Genome Oligonucleotide Microarrays  

Microsoft Academic Search

Complete genome sequences in combination with global screening methods allow parallel analysis of multiple mutant loci to determine the requirement for specific genes in different environments. In this paper we describe a high-definition microarray approach for investigating the growth effects of Tn5 insertions in Escherichia coli K-12. Libraries of insertion mutants generated by a unique Tn5 mutagenesis system were grown

Kelly M. Winterberg; John Luecke; Amanda S. Bruegl; William S. Reznikoff

2005-01-01

209

Identification of Genes Involved in Swarming Motility Using a Pseudomonas aeruginosa PAO1 Mini-Tn5-lux Mutant Library  

Microsoft Academic Search

During a screening of a mini-Tn5-luxCDABE transposon mutant library of Pseudomonas aeruginosa PAO1 for alterations in swarming motility, 36 mutants were identified with Tn5 insertions in genes for the synthesis or function of flagellin and type IV pilus, in genes for the Xcp-related type II secretion system, and in regulatory, metabolic, chemosensory, and hypothetical genes with unknown functions. These mutants

Joerg Overhage; Shawn Lewenza; Alexandra K. Marr; Robert E. W. Hancock

2007-01-01

210

A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn 917 insertions  

Microsoft Academic Search

A rapid and general procedure has been devised for the pBR322-mediated cloning in Escherichia coli of Bacillus subtilis chromosomal DNA extending in a specified direction from any Tn917 insertion. Derivatives of Tn917 have been constructed that contain a pBR322-derived replicon, together with a chloramphenicol-resistance (Cmr) gene of Gram-positive origin (selectable in B. subtilis), inserted by ligation in two orientations into

Philip Youngman; John B. Perkins; Richard Losick

1984-01-01

211

Performance characteristics of the Erenna™ cTnI assay for in vitro diagnostic use in the Singulex® Clinical Laboratory  

Microsoft Academic Search

Background: Measurement of cardiac Troponin-I (cTnI) has been designated the gold standard for diagnosis of acute myocardial infarction and as indicative of cardiac disease. Measurement of cTnI can be used for diagnosis of acute myocardial infarction and in the risk stratification of patients with non-ST segment elevation acute coronary syndromes, with respect to relative risk of mortality, myocardial infarction, or

Saker Karimi; Peter Simpson; Khanh Bui; Emerson Quan; Loan Le; Jenny Hsu; Sara Agee; Guido Baechler

212

Snohomish County Biodiesel Project  

SciTech Connect

Snohomish County in western Washington State began converting its vehicle fleet to use a blend of biodiesel and petroleum diesel in 2005. As prices for biodiesel rose due to increased demand for this cleaner-burning fuel, Snohomish County looked to its farmers to ���¢��������grow���¢������� this fuel locally. Suitable seed crops that can be crushed to extract oil for use as biodiesel feedstock include canola, mustard, and camelina. The residue, or mash, has high value as an animal feed. County farmers began with 52 acres of canola and mustard crops in 2006, increasing to 250 acres and 356 tons in 2008. In 2009, this number decreased to about 150 acres and 300 tons due to increased price for mustard seed.

Terrill Chang; Deanna Carveth

2010-02-01

213

County Waterford Image Archive  

NSDL National Science Digital Library

Using volunteer help and a locally written image management system, the Waterford County Museum in Ireland has created this collection of close to 3,000 historical images that document Waterford County and its parishes from the late 1890s to the present. The Web interface to the collection includes a number of helpful features - for those familiar with Waterford county and its place and family names, there is a search box, but for those less familiar there are several ways to browse the collection, by selecting photographer, location, image format, or subjects from drop down lists, as well as selecting parishes from a map to see the pictures associated with each. A photo of the week is posted on the site, and visitors can sign up to receive an RSS feed or, alternatively, an email message when new images are added. There are also easy ways to submit photographs for inclusion in the archive, and for locals to contribute identifying information.

214

A Functional Origin of Transfer (oriT) on the Conjugative Transposon Tn916  

Microsoft Academic Search

Theoriginoftransfer(oriT)ofthe18-kbconjugativetransposonTn916hasbeenlocalizedtoa466-bpregion which spans nucleotides 15215 to 15681 on the transposon map. TheoriTlies within an intercistronic region between open reading frames ORF20 and ORF21 that contains six sets of inverted repeats ranging from 10 to 20bpinsize.Thesegmentcontainsthreesequencesshowingidentityin9of12bptotheconsensusnickingsite (nic) of the IncP family of conjugative plasmids found in gram-negative bacteria. Overlapping one of these sequences is a region similar to the nic site

DEBORAH D. JAWORSKI; ANDDON B. CLEWELL

1995-01-01

215

Characterization of a transposon Tn916-generated mutant of Haemophilus ducreyi 35000 defective in lipooligosaccharide biosynthesis.  

PubMed Central

To define the role of the surface lipooligosaccharide (LOS) of Haemophilus ducreyi in the pathogenesis of chancroid, Tn916 mutants of H. ducreyi 35000 defective in expression of the murine monoclonal antibody (MAb) 3F11 epitope on H. ducreyi LOS were identified by immunologic screening. One mutant, designated 1381, has an LOS which lacks the MAb 3F11 epitope and migrates with an increased mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The gene disrupted by the Tn916 element in strain 1381 was identified by cloning the sequences flanking the Tn916 element. The sequences were then used to probe a lambda DASHII genomic library. In strain 1381, Tn916 interrupts a gene which encodes an open reading frame (ORF) with an Mr of 40,246. This ORF has homology to the product of the rfaK gene of Escherichia coli. The major LOS glycoform produced by strain 1381 was analyzed by using a combination of mass spectrometry, linkage and composition analysis, and 1H nuclear magnetic resonance spectroscopy. The major LOS species was found to terminate in a single glucose attached to the heptose (L-glycero-D-manno-heptose, or Hep) trisaccharide core. In the wild-type strain 35000, glucose serves as the acceptor for the addition of the D-glycero-D-manno-heptose (or DDHep), which extends to form the mature branch of the H. ducreyi LOS. This mature oligosaccharide is in turn partially capped by the addition of sialic acid (NeuAc), i.e., NeuAc2 alpha-->3Gal beta1-->4GlcNAc beta1-->3Gal beta1-->4DDHep alpha1-->6Glc beta1 (W. Melaugh et al., Biochemistry 33:13070-13078, 1994). Since this LOS terminates prior to the addition of the branch DD-heptose, this gene is likely to encode the D-glycero-D-manno-heptosyltransferase. Strain 1381 exhibits a significant reduction in adherence to and invasion of primary human keratinocytes. This defect was complemented by the cloned heptosyltransferase gene, indicating that the terminal portion of the LOS oligosaccharide plays an important role in adherence to human keratinocytes.

Gibson, B W; Campagnari, A A; Melaugh, W; Phillips, N J; Apicella, M A; Grass, S; Wang, J; Palmer, K L; Munson, R S

1997-01-01

216

Imperial County geothermal development  

NASA Astrophysics Data System (ADS)

The progress of geothermal development during the past 3 years, county activities in support of geothermal development, and current challenges and future needs of the geothermal industry and the county are summarized. Three additional Known Geothermal Resources Areas (KGRAs) were identified: the Westmorland KGRA, the East Brawley KGRA, and the South Brawley KGRA. Three 10 megawatt power plants began operations during the grant period. Three commercial power plants are scheduled to begin construction during late 1982 or early 1983. Site work was begun for the Heber Flash Power Plant. Two commercial power plants are in planning stages.

1982-10-01

217

Monroe County Urban Accessibility Study.  

National Technical Information Service (NTIS)

The objectives of this study were to survey primary ambulatory health services in Monroe County, N.Y., and to estimate the accessibility of these services to the county's population. Community group meetings were used to collect information on organizatio...

1975-01-01

218

Enhanced self-association of mucins possessing the T and Tn carbohydrate cancer antigens at the single-molecule level.  

PubMed

Mucins are linear O-glycosylated glycoproteins involved in inflammation, cell adhesion, and tumorigenesis. Cancer-associated mucins often possess increased expression of the T (Gal?1,3GalNAc?Thr/Ser) and Tn (GalNAc?Thr/Ser) cancer antigens, which are diagnostic markers for several cancers, including colon cancer. We have used AFM based single-molecule forced unbinding under near physiological conditions to investigate the self-interactions between porcine submaxillary mucin (PSM) as well as between PSM analogs possessing various carbohydrates including the T- and Tn-antigen. Distributions of unbinding forces and corresponding force loading rates were determined for force loading rates from 0.18 nN/s to 39 nN/s, and processed to yield most probable unbinding forces f* and lifetimes of the interactions. Parameter f* varied in the range 27 to 50 pN at force loading rates of about 2 nN/s among the various mucins. All mucin samples investigated showed self-interaction, but the tendency was greatest for PSM displaying only the Tn-antigen (Tn-PSM) or a mixture of Tn-, T-antigen, and the trisaccharide Fuc?1,2Gal?1,3GalNAc (Tri-PSM). Weaker self-interactions were observed for native PSM (Fd-PSM), which consists of a nearly equal mixture of the longer core 1 blood group A tetrasaccharide (GalNAc?1,3(Fuc?1,2)Gal?1,3GalNAc?Ser/Thr) and Tn-antigen. The data are consistent with the truncated Tn and T glycans enhancing self-interaction of the mucins. These carbohydrate cancer antigens may, thus, play an active role in the disease by constitutively activating mucin and mucin-type receptors by self-association on cells. PMID:22428527

Haugstad, Kristin E; Gerken, Thomas A; Stokke, Bjørn T; Dam, Tarun K; Brewer, C Fred; Sletmoen, Marit

2012-04-03

219

Comparable susceptibilities of human 293 cells and insect Tn-5B1-4 cells to photoactivated alpha-terthienyl.  

PubMed

The hope is that photoactive compounds acting as potential insecticides will have reduced environmental risk, but that is not necessarily the case. In an attempt to elucidate the risk by which photoactivated alpha-terthienyl (alpha-T) affects human health, the effects of exposure of human 293 cells and insect Tn-5B1-4 cells to photoactivated alpha-T at certain doses were characterized. Photoactivated alpha-T exhibited dose dependence of toxicity and time kinetics of phototoxic activation on the growth of 293 cells (EC(50) = 6.23 microg/mL) and Tn-5B1-4 cells (EC(50) = 3.36 microg/mL). 293 cells appeared to be anchorage-independent, inflated, and broken; Tn-5B1-4 cells showed significant necrosis. ROS productions and lipid peroxidation of 293 cells were always lower than that of Tn-5B1-4 cells in the treatments of alpha-T at the same dose. Moreover, photoactivated alpha-T caused nonselective DNA damage in 293 and Tn-5B1-4 cells at a 10 microg/mL dose and induced cell-cycle progression of 293 cells to increase apoptosis of cells and G1 arrest and decrease in S phase cell population, whereas Tn-5B1-4 cells showed S arrest accompanied by a dose-dependent decrease in G1 and G2 phase cells at a 5 microg/mL dose. These observations suggest that Tn-5B1-4 cells are more susceptible to the action of photoactivated alpha-T than 293 cells, but photoactivated alpha-T as an efficient insecticide might be a potential factor in human mutagenic progression. PMID:20014764

Huang, Qingchun; Liu, Yang; Zhan, Taisong; Deng, Yunfei; He, Yuan

2010-03-10

220

The Sugar Creek zinc deposit, Jackson Co. TN -- Exploration history, geology and mineralization  

SciTech Connect

During the 60's and 70's zinc exploration of central TN and KY was active. The Sugar Creek Project was one of several investigated by Exxon. The discovery hole, Cu 15, was drilled in early 1973. The Sugar Creek Zinc Deposit was acquired by Independence Mining Co. in 1986 and I.M.C. has subsequently completed additional drilling, both stepout and confirmation holes. A total of 137 holes for 300,833 ft have been drilled. The Sugar Creek deposit is a typical Tennessee zinc deposit (Mississippi Valley Type) which occurs in solution collapse breccias in the Lower Ordovician, Knox Dolomite. The Knox consists of fine grained dolomite with interlayered limestones and crystalline dolomite. Only scattered residual limestone is found in the Sugar Creek area. Collapse breccias have formed which control zinc deposition and are similar to other TN Zn. deposits. At Sugar Creek the types of breccias include: a vertically exaggerated glory hole breakthrough breccia which extends to within 137 ft. of the Knox unconformity, has 500 ft. of zinc mineralization with 8 significant zinc intervals; holes with stacked zinc intervals interpreted to be sides of breakthrough breccia; and single zinc intervals in laterally positioned bedded mineral zones. A total of 99 holes were drilled in the more intense mineralized areas. The ratio of ore to non ore holes is nearly 1 to 1. The mineralization is typical M.V.T. with predominantly sphalerite and only minor occurrences of galena, fluorite, pyrite, etc.

Reinbold, G.; Moran, A.V.; Stevens, D.L. (Independence Mining Co. Inc., Reno, NV (United States))

1993-03-01

221

A novel dihydrofolate reductase cassette inserted in an integron borne on a Tn21-like element.  

PubMed Central

In this study, a 498-bp dhfrXII gene coding for trimethoprim resistance was found inserted in a cassette-like manner in the recombinationally active locus, the integron, borne on a transposon Tn21-like element. The dhfrXII cassette is distinct from those cassettes earlier observed in integrons and was found here upstream of two similarly inserted cassettes. The second one carried the new unidentified orfF, which is 85% identical to the orfD cassette in R46. The third cassette contained the aadA2 gene mediating spectinomycin resistance. The plasmid carrying this Tn21-like element was originally isolated from a trimethoprim-resistant urinary tract pathogen, Escherichia coli, from Turku City Hospital, Turku, Finland. By colony hybridization and polymerase chain reaction, this group of three cassettes, including dhfrXII, was detected in four additional E. coli strains of similar origin and in four Shigella strains isolated in Finland but originating from Asia. The dihydrofolate reductase produced from dhfrXII showed an unusual drug resistance in that 50% of the enzymatic activity remained at a trimethoprim concentration of 1 mM.

Heikkila, E; Skurnik, M; Sundstrom, L; Huovinen, P

1993-01-01

222

Bioactive secondary metabolites from a new terrestrial Streptomyces sp. TN262.  

PubMed

During our search for Streptomyces spp. as new producers of bioactive secondary metabolites, the ethyl acetate extract of the new terrestrial Streptomyces isolate TN262 delivered eight antimicrobially active compounds. They were identified as 1-acetyl-beta-carboline (1), tryptophol (2), cineromycin B (3), 2,3-dihydrocineromycin B (4), cyclo-(tyrosylprolyl) (5), 3-(hydroxyacetyl)-indole (6), brevianamide F (7), and cis-cyclo-(L-prolyl-L-leucyl) (8). Three further metabolites were detected in the unpolar fractions using GC-MS and tentatively assigned as benzophenone (9), N-butyl-benzenesulfonamide (10), and hexanedioic acid-bis-(2-ethylhexyl) ester (11). This last compound is known as plasticizer derivatives, but it has never been described from natural sources. In this article, we describe the identification of the new Streptomyces sp. isolate TN262 using its cultural characteristics, the nucleotide sequence of the corresponding 16S rRNA gene and the phylogenetic analysis, followed by optimization, large-scale fermentation, isolation of the bioactive constituents, and determination of their structures. The biological activity of compounds (2), (3), (4), and those of the unpolar fractions was addressed as well. PMID:19842066

Elleuch, Lobna; Shaaban, Mohamed; Smaoui, Slim; Mellouli, Lotfi; Karray-Rebai, Ines; Fourati-Ben Fguira, Lilia; Shaaban, Khaled A; Laatsch, Hartmut

2009-10-20

223

Board of County Commissioners  

Microsoft Academic Search

Analysis: In an effort to more effectively respond to, investigate and where needed arrest child abuse offenders, the Wakulla County Sheriff's Office is requesting the funding of a Persons Crime Unit. The Persons Crime Investigator is a certified law enforcement officer who is trained in handling domestic violence cases, sex crimes on children and child abuse cases. The Persons Crime

Sheriff David Harvey

2008-01-01

224

Dade County: Paradise Lost?  

ERIC Educational Resources Information Center

A Dade county commissioner discusses problems that the greater Miami, Florida, area is facing due to the recent influx of Cuban and Haitian refugees. Calls for federal action in the areas of immigration policy, aid for the unemployed, and increased social services. (GC)

Shack, Ruth

1980-01-01

225

STATE/COUNTY BORDERS  

EPA Science Inventory

This data is available for the entire region. The level of detail is primarily suited for region/state/county/basin sized maps. The borders are not accurate enough for small areas like sub-basins and site areas. We use this coverage for general backgrounds and borders only. T...

226

Diversity of Tn1546 and its role in the dissemination of vancomycin-resistant enterococci in Portugal.  

PubMed

We characterized the molecular diversity of vanA vancomycin-resistant enterococci (VRE; 176 isolates/87 pulsed-field gel electrophoresis types) from different sources and cities in Portugal (1996 to 2004): (i) food animals (FA; n = 38 isolates out of 31 samples), hospitalized humans (HH; n = 101/101), healthy human volunteers (HV; n = 7/4), and environmental sources (n = 30/10). Some strains were isolated from different hosts and persistently recovered for years. Twenty-four Tn1546 variants were identified, all located on plasmids (30 to 250 kb). Some Tn1546 variants were associated with specific sources such as FA (3 types), HH (11 types), or HV (1 type), while others were recovered from isolates of different origins (8 types). Polymorphisms in the central vanRSHA region of Tn1546 were scarcely detected, while alterations upstream of vanR and downstream of vanA were frequently identified involving mutations (vanS and vanX), deletions (vanY), insertions (IS1216V, ISEf1, and IS19; sequences with or without homology with others available in GenBank databases), and different genetic rearrangements. Most Tn1546 variants contained IS1216V (14 types) or ISEf1 (6 types). IS1216V was found alone or associated with an IS3-like element at different orientations and positions in Tn1546 from human, animal, and environmental samples. ISEf1 was located within vanX-vanY region at nucleotide 9044 of Tn1546 variants mostly associated with clinical isolates, suggesting a common genetic platform. IS19 was observed within the vanX-vanY region in one Tn1546 variant from poultry. Recent spread of VRE in Portugal reflects a complex epidemiology involving both clonal spread and plasmid dissemination containing a variety of Tn1546 types. Apparent Tn1546 heterogeneity among enterococci from human, animal, and environmental sources might reflect frequent genetic exchange events and evolution of particular widely disseminated genetic elements. PMID:18180362

Novais, Carla; Freitas, Ana R; Sousa, João C; Baquero, Fernando; Coque, Teresa M; Peixe, Luísa V

2008-01-07

227

Diversity of Tn1546 and Its Role in the Dissemination of Vancomycin-Resistant Enterococci in Portugal?  

PubMed Central

We characterized the molecular diversity of vanA vancomycin-resistant enterococci (VRE; 176 isolates/87 pulsed-field gel electrophoresis types) from different sources and cities in Portugal (1996 to 2004): (i) food animals (FA; n = 38 isolates out of 31 samples), hospitalized humans (HH; n = 101/101), healthy human volunteers (HV; n = 7/4), and environmental sources (n = 30/10). Some strains were isolated from different hosts and persistently recovered for years. Twenty-four Tn1546 variants were identified, all located on plasmids (30 to 250 kb). Some Tn1546 variants were associated with specific sources such as FA (3 types), HH (11 types), or HV (1 type), while others were recovered from isolates of different origins (8 types). Polymorphisms in the central vanRSHA region of Tn1546 were scarcely detected, while alterations upstream of vanR and downstream of vanA were frequently identified involving mutations (vanS and vanX), deletions (vanY), insertions (IS1216V, ISEf1, and IS19; sequences with or without homology with others available in GenBank databases), and different genetic rearrangements. Most Tn1546 variants contained IS1216V (14 types) or ISEf1 (6 types). IS1216V was found alone or associated with an IS3-like element at different orientations and positions in Tn1546 from human, animal, and environmental samples. ISEf1 was located within vanX-vanY region at nucleotide 9044 of Tn1546 variants mostly associated with clinical isolates, suggesting a common genetic platform. IS19 was observed within the vanX-vanY region in one Tn1546 variant from poultry. Recent spread of VRE in Portugal reflects a complex epidemiology involving both clonal spread and plasmid dissemination containing a variety of Tn1546 types. Apparent Tn1546 heterogeneity among enterococci from human, animal, and environmental sources might reflect frequent genetic exchange events and evolution of particular widely disseminated genetic elements.

Novais, Carla; Freitas, Ana R.; Sousa, Joao C.; Baquero, Fernando; Coque, Teresa M.; Peixe, Luisa V.

2008-01-01

228

An in silico evaluation of Tn916 as a tool for generalized mutagenesis in Haemophilus influenzae Rd.  

PubMed

The transposon Tn916 was evaluated as a tool for generalized mutagenesis of the genome of Haemophilus influenzae. This was achieved in silico by searching the genome sequence of H. influenzae Rd for the published Tn916 target site consensus sequence 5' TT/ATTTT(N)6AAAAAA/TA. This search identified 16 putative target sites. In subsequent experiments, integration of Tn916 did not occur at any of these sites. Using the nucleotide sequences of these observed integration sites, a new consensus sequence, 5' TTTTT(N)xAAAAA (4 < or = x < or = 7), was derived. This sequence reflects the curve-twist-curve DNA topology which is a feature common to all Tn916 integration sites. A search of the H. influenzae Rd genome using the new consensus sequence identified 167 potential target sites, representing approximately 1% of the total genome. Only 80 of these sites were located within ORFs. The presence of such a limited number of target sites places severe constraints on the use of Tn916 as a tool for generalized mutagenesis of the genome of H. influenzae. PMID:9782500

Hosking, S L; Deadman, M E; Moxon, E R; Peden, J F; Saunders, N J; High, N J

1998-09-01

229

Identification and cloning of genes from Porphyromonas gingivalis after mutagenesis with a modified Tn4400 transposon from Bacteroides fragilis.  

PubMed

Porphyromonas gingivalis is a gram-negative, black-pigmented, oral anaerobe strongly associated with adult periodontitis. Previous transposon mutagenesis studies with this organism were based on the Bacteroides transposon Tn4351. Characterization of Tn4351-disrupted genes by cloning has not been an efficient way to analyze large numbers of mutants and is further complicated by the high rate of cointegration of the suicide delivery vector containing Tn4351. In this study, we mutagenized P. gingivalis with a modified version of the Bacteroides fragilis transposon Tn4400. Plasmid pYT646B carrying the transposon was mobilized from Escherichia coli to P. gingivalis ATCC 33277 by conjugation. Both normal and inverse transposition frequencies were similar (3 x 10(-8)). However, the inverse transposon (Tn4400') contains a pBR322 replicon and a beta-lactamase gene; thus, the cloning of disrupted genomic DNAs from inverse transposition mutants was easily accomplished after ligation of genomic fragments and transformation into E. coli. Thousands of transconjugants could be obtained in a single mating experiment, and inverse transposition was random as demonstrated by Southern hybridization. By this procedure the disrupted genes from P. gingivalis pleiotropic mutants were quickly cloned, sequenced, and identified. PMID:10603421

Chen, T; Dong, H; Tang, Y P; Dallas, M M; Malamy, M H; Duncan, M J

2000-01-01

230

Glycan elongation beyond the mucin associated tn antigen protects tumor cells from immune-mediated killing.  

PubMed

Membrane bound mucins are up-regulated and aberrantly glycosylated during malignant transformation in many cancer cells. This results in a negatively charged glycoprotein coat which may protect cancer cells from immune surveillance. However, only limited data have so far demonstrated the critical steps in glycan elongation that make aberrantly glycosylated mucins affect the interaction between cancer cells and cytotoxic effector cells of the immune system. Tn (GalNAc-Ser/Thr), STn (NeuAc?2-6GalNAc-Ser/Thr), T (Gal?1-3GalNAc-Ser/Thr), and ST (NeuAc?2-6Gal?1-3GalNAc-Ser/Thr) antigens are recognized as cancer associated truncated glycans, and are expressed in many adenocarcinomas, e.g. breast- and pancreatic cancer cells. To investigate the role of the cancer associated glycan truncations in immune-mediated killing we created glyco-engineered breast- and pancreatic cancer cells expressing only the shortest possible mucin-like glycans (Tn and STn). Glyco-engineering was performed by zinc finger nuclease (ZFN) knockout (KO) of the Core 1 enzyme chaperone COSMC, thereby preventing glycan elongation beyond the initial GalNAc residue in O-linked glycans. We find that COSMC KO in the breast and pancreatic cancer cell lines T47D and Capan-1 increases sensitivity to both NK cell mediated antibody-dependent cellular-cytotoxicity (ADCC) and cytotoxic T lymphocyte (CTL)-mediated killing. In addition, we investigated the association between total cell surface expression of MUC1/MUC16 and NK or CTL mediated killing, and observed an inverse correlation between MUC16/MUC1 expression and the sensitivity to ADCC and CTL-mediated killing. Together, these data suggest that up-regulation of membrane bound mucins protects cells from immune mediated killing, and that particular glycosylation steps, as demonstrated for glycan elongation beyond Tn and STn, can be important for fine tuning of the immune escape mechanisms in cancer cells. PMID:24039759

Madsen, Caroline B; Lavrsen, Kirstine; Steentoft, Catharina; Vester-Christensen, Malene B; Clausen, Henrik; Wandall, Hans H; Pedersen, Anders Elm

2013-09-06

231

Glycan Elongation Beyond the Mucin Associated Tn Antigen Protects Tumor Cells from Immune-Mediated Killing  

PubMed Central

Membrane bound mucins are up-regulated and aberrantly glycosylated during malignant transformation in many cancer cells. This results in a negatively charged glycoprotein coat which may protect cancer cells from immune surveillance. However, only limited data have so far demonstrated the critical steps in glycan elongation that make aberrantly glycosylated mucins affect the interaction between cancer cells and cytotoxic effector cells of the immune system. Tn (GalNAc-Ser/Thr), STn (NeuAc?2-6GalNAc-Ser/Thr), T (Gal?1–3GalNAc-Ser/Thr), and ST (NeuAc?2-6Gal?1–3GalNAc-Ser/Thr) antigens are recognized as cancer associated truncated glycans, and are expressed in many adenocarcinomas, e.g. breast- and pancreatic cancer cells. To investigate the role of the cancer associated glycan truncations in immune-mediated killing we created glyco-engineered breast- and pancreatic cancer cells expressing only the shortest possible mucin-like glycans (Tn and STn). Glyco-engineering was performed by zinc finger nuclease (ZFN) knockout (KO) of the Core 1 enzyme chaperone COSMC, thereby preventing glycan elongation beyond the initial GalNAc residue in O-linked glycans. We find that COSMC KO in the breast and pancreatic cancer cell lines T47D and Capan-1 increases sensitivity to both NK cell mediated antibody-dependent cellular-cytotoxicity (ADCC) and cytotoxic T lymphocyte (CTL)-mediated killing. In addition, we investigated the association between total cell surface expression of MUC1/MUC16 and NK or CTL mediated killing, and observed an inverse correlation between MUC16/MUC1 expression and the sensitivity to ADCC and CTL-mediated killing. Together, these data suggest that up-regulation of membrane bound mucins protects cells from immune mediated killing, and that particular glycosylation steps, as demonstrated for glycan elongation beyond Tn and STn, can be important for fine tuning of the immune escape mechanisms in cancer cells.

Madsen, Caroline B.; Lavrsen, Kirstine; Steentoft, Catharina; Vester-Christensen, Malene B.; Clausen, Henrik

2013-01-01

232

RUNOFF PROPERTIES OF SS, TN, AND TP AND POSSIBILITY OF THEIR CONTROL BY FLOCCULATION IN AGRICULTURAL AREAS IN IWATE  

NASA Astrophysics Data System (ADS)

We investigated the runoff properties of SS, TN, and TP in the Mimitori River and the Takizawa River streaming in agricultural areas in Iwate prefecture, Japan. Field observations provide that the following findings: 1) the concentrations of SS, TN, and TP increase in paddling and transplanting period in paddy fields, 2) TN and TP concentrations strongly correlate with SS, 3) the loads of SS and nutrients are much higher during rainfall time. To examine the possibility to control SS sorbing nutrients, we carried out experimental studies on the charging and flocculation of the suspended particles as well as the resuspension of flocculated sediments. The results clarified that the flocculation behaviors qualitatively follow the DLVO theory and the resuspension behaviors are similar to those of natural sediments. Finally, we suggest that increasing calcium ions over 2mM is effective to settle down the particles.

Kobayashi, Motoyoshi; Nitanai, Miki; Yamada, Kenta; Abe, Tsutomu; Harashina, Koji

233

Evolutionary relationship between Tn 21 -like elements and pBP201, a plasmid from Klebsiella pneumoniae mediating resistance to gentamicin and eight other drugs  

Microsoft Academic Search

We have characterized pBP201 one of the plasmids from a collection of 46 strains producing adenylyltransferase ANT (2?) (Schmidt 1984). It confers resistance to sulphonamides and produces aminoglycoside adenylyltransferases AAD (3?) and ANT (2?) and ß-lactamase TEM-1. Plasmid pBP201 has a size of 24.8 kilobases (kb) and contains TnA and a Tn21-related element, Tn4000?, with deletions in mer and the

Friedrich Schmidt; Ingrid Klopfer-Kaul

1984-01-01

234

A quantitative real-time PCR assay for the detection of tetR of Tn 10 in Escherichia coli using SYBR Green and the Opticon  

Microsoft Academic Search

Bacteria of implant infections are extremely resistant to antibiotics. One reason for this antibiotic resistance are transposons; the well-known transposon Tn10, for example, mediates tetracycline resistance to Escherichia coli. Two genes of Tn10, tetA and tetR, are essential for the mechanism of resistance. These genes encode a drug-specific efflux protein and a tetracycline repressor protein, respectively. Tn10 is also widely

Christian Morsczeck; Daniel Langendörfer; Jörg Michael Schierholz

2004-01-01

235

Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosomes deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR1  

Microsoft Academic Search

A magnetosome deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1 was generated by mini-Tn5 transposon mutagenesis, and a 5045-bp fragment flanking mini-Tn5 in NM4 was cloned by Anchored\\u000a PCR. Sequencing analysis showed that this fragment involved six putative open reading frames (ORFs); the mini-Tn5 was inserted\\u000a into ORF4. Functional complementary test indicated that the 5045-bp fragment was required for biosynthesis of

Feng Li; Ying Li; Wei Jiang; Zhenfang Wang; Jilun Li

2005-01-01

236

Tn10-encoded tet repressor can regulate an operator-containing plant promoter.  

PubMed Central

The Tn10-encoded tet repressor-operator system was used to regulate transcription from the cauliflower mosaic virus (CaMV) 35S promoter. Expression was monitored in a transient assay system by using electric field-mediated gene transfer ("electroporation") into tobacco protoplasts. The tet repressor, being expressed in the plant cells under the control of eukaryotic transcription signals, blocks transcription of a CaMV 35S promoter chloramphenicol acetyltransferase (cat) fusion gene when the two tet operators flank the "TATA" box. In the presence of the inducer tetracycline, expression is restored to full activity. Location of the operators 21 base pairs downstream of the transcription start site does not significantly affect transcription in the presence of the repressor. These experiments show that a prokaryotic regulatory protein can function in plant cells. The tet repressor-operator complex may be useful for specifically inducing transferred genes at different stages of plant development. Images

Gatz, C; Quail, P H

1988-01-01

237

Transposon Tn5-induced mutagenesis of Rhizobium japonicum yielding a wide variety of mutants.  

PubMed Central

When the "suicide" vector pSUP1011, which carries transposon Tn5 (Kmr), was introduced into Rhizobium japonicum USDA 110, kanamycin-resistant (Kmr) colonies were detected at a frequency (4.2 X 10-6) ca. 30 times greater than the spontaneous kanamycin resistance frequency (1.4 X 10-7). Ten thousand Kmr mutants were isolated and tested for nutritional auxotrophy. Auxotrophs were detected at a frequency of 0.5%. The following classes of auxotrophs were identified: adenine- (three), histidine- (three), glutamate- (five), adenine plus thiamine- (nine), uracil- (three), pantothenic acid- (one), tryptophan- (three), and methionine- (three). Mutants blocked in symbiotic nitrogen fixation (Fix-) were also identified at a frequency of 3%. The glutamate auxotrophs were studied in more detail, and all five showed an altered expression of nitrogenase activity in free-living cultures.

Hom, S S; Uratsu, S L; Hoang, F

1984-01-01

238

Resistance of a Tn4351-generated polysaccharide mutant of Porphyromonas gingivalis to polymorphonuclear leukocyte killing.  

PubMed Central

In this study, we describe the development of an efficient transpositional mutagenesis system for Porphyromonas gingivalis using the Bacteroides fragilis transposon Tn4351. Using this system, we have isolated and characterized a Tn4351-generated mutant of P. gingivalis A7436, designated MSM-1, which exhibits enhanced resistance to polymorphonuclear leukocyte (PMN) phagocytosis and killing. P. gingivalis MSM-1 was initially selected based on its colony morphology; MSM-1 appeared as a mucoid, beige-pigmented colony. Analysis of P. gingivalis MSM-1 by electron microscopy and staining with ruthenium red revealed the presence of a thick ruthenium red-staining layer that was twice the thickness of this layer observed in the parent strain. P. gingivalis MSM-1 was found to be more hydrophilic than strain A7436 by hydrocarbon partitioning. Analysis of phenol-water extracts prepared from P. gingivalis A7436 and MSM-1 by Western (immunoblot) analysis and immunodiffusion with hyperimmune sera raised against A7436 and MSM-1 revealed the loss of a high-molecular-weight anionic polysaccharide component in extracts prepared from MSM-1. P. gingivalis MSM-1 was also found to be more resistant to PMN phagocytosis and intracellular killing than the parent strain, as assessed in a fluorochrome phagocytosis microassay. These differences were statistically significant (P < 0.05) when comparing PMN phagocytosis in nonimmune serum and intracellular killing in nonimmune and immune sera. P. gingivalis MSM-1 was also more resistant to killing by crude granule extracts from PMNs than was P. gingivalis A7436. These results indicate that the increased evasion of PMN phagocytosis and killing exhibited by P. gingivalis MSM-1 may result from alterations in polysaccharide-containing antigens.

Genco, C A; Schifferle, R E; Njoroge, T; Forng, R Y; Cutler, C W

1995-01-01

239

75 FR 430 - Notice of Intent To Prepare an Environmental Impact Statement for the Sigurd-Red Butte...  

Federal Register 2010, 2011, 2012, 2013

...in preparation of the EIS. Currently, the U.S. Forest Service (Dixie and Fishlake National Forests), State of Utah, Millard County, Sevier County, Beaver County, Utah Division of Wildlife Resources, City of St. George, and City of...

2010-01-05

240

62 FR 27169 - Irish Potatoes Grown in Modoc and Siskiyou Counties, California, and in all Counties in Oregon...  

Federal Register 2010, 2011, 2012, 2013

...Siskiyou Counties, California, and in all Counties in Oregon, Except Malheur County...Siskiyou Counties, California, and in all counties in Oregon, except Malheur County...rate as issued herein will be applicable to all assessable potatoes beginning July 1,...

1997-05-19

241

78 FR 43827 - Irish Potatoes Grown in Modoc and Siskiyou Counties, California, and in All Counties in Oregon...  

Federal Register 2010, 2011, 2012, 2013

...Siskiyou Counties, California, and in All Counties in Oregon, Except Malheur County...Siskiyou Counties, California, and in all counties in Oregon, except Malheur County...at: http://www.regulations.gov. All comments submitted in response to this...

2013-07-22

242

Modular evolution of TnGBSs, a new family of integrative and conjugative elements associating insertion sequence transposition, plasmid replication, and conjugation for their spreading.  

PubMed

Integrative and conjugative elements (ICEs) have a major impact on gene flow and genome dynamics in bacteria. The ICEs TnGBS1 and TnGBS2, first identified in Streptococcus agalactiae, use a DDE transposase, unlike most characterized ICEs, which depend on a phage-like integrase for their mobility. Here we identified 56 additional TnGBS-related ICEs by systematic genome analysis. Interestingly, all except one are inserted in streptococcal genomes. Sequence comparison of the proteins conserved among these ICEs defined two subtypes related to TnGBS1 or TnGBS2. We showed that both types encode different conjugation modules: a type IV secretion system, a VirD4 coupling protein, and a relaxase and its cognate oriT site, shared with distinct lineages of conjugative elements of Firmicutes. Phylogenetic analysis suggested that TnGBSs evolved from two conjugative elements of different origins by the successive recruitment of a transposition module derived from insertion sequences (ISs). Furthermore, TnGBSs share replication modules with different plasmids. Mutational analyses and conjugation experiments showed that TnGBS1 and TnGBS2 combine replication and transposition upstream promoters for their transfer and stabilization. Despite an evolutionarily successful horizontal dissemination within the genus Streptococcus, these ICEs have a restricted host range. However, we reveal that for TnGBS1 and TnGBS2, this host restriction is not due to a transfer incompatibility linked to the conjugation machineries but most likely to their ability for transient maintenance through replication after their transfer. PMID:23435978

Guérillot, Romain; Da Cunha, Violette; Sauvage, Elisabeth; Bouchier, Christiane; Glaser, Philippe

2013-02-22

243

Heritage Awareness in County Wicklow  

Microsoft Academic Search

This research project investigated the community’s current level of heritage awareness in County Wicklow. The study was initiated by Wicklow County Council and the Heritage Council in response to objective 1, action 1.2 of the County Wicklow heritage plan 2004-2008, which pointed to the need to undertake a study to determine public attitudes towards heritage and to gauge the current

Anne Dagg

2008-01-01

244

Chlorobenzoate Catabolic Transposon Tn5271 is a Composite Class I Element with Flanking Class II Insertion Sequences  

Microsoft Academic Search

The structure of a transposon specifying the biodegradation of chlorobenzoate contaminants is described. Tn5271 is a 17-kilobase (kb) transposon that resides in the plasmid or chromosome of Alcaligenes sp. strain BR60 and allows this organism to grow on 3- and 4-chlorobenzoate. The transposon is flanked by a directly repeated sequence of 3201 base pairs (bp), which in turn is flanked

Cindy Nakatsu; James Ng; Rama Singh; Neil Straus; Campbell Wyndham

1991-01-01

245

RADIOCARBON MEASUREMENT OF THE BIOGENIC CONTRIBUTION TO SUMMERTIME PM 2.5 AMBIENT AEROSOL IN NASHVILLE, TN  

EPA Science Inventory

Radiocarbon (14C) measurements performed on PM-2.5 samples collected near Nashville, TN from June 21 to July 13, 1999, showed high levels of modern carbon, ranging from 56 to 80% of the total carbon in the samples. Radiocarbon measurements performed on dichloromethane extracts of...

246

78 FR 25403 - Proposed Amendment of Class E Airspace; Dayton, TN, Establishment of Class E Airspace; Cleveland...  

Federal Register 2010, 2011, 2012, 2013

...global positioning system (GPS) SIAPs at the...the Docket Management System (see ADDRESSES section...Rulemaking distribution System, which describes the...Airport as the airport has closed. This proposal also...Above the Surface of the Earth. * * * * * ASO TN...

2013-05-01

247

Correlating Calcium Binding, F?rster Resonance Energy Transfer, and Conformational Change in the Biosensor TN-XXL  

PubMed Central

Genetically encoded calcium indicators have become instrumental in imaging signaling in complex tissues and neuronal circuits in vivo. Despite their importance, structure-function relationships of these sensors often remain largely uncharacterized due to their artificial and multimodular composition. Here, we describe a combination of protein engineering and kinetic, spectroscopic, and biophysical analysis of the Förster resonance energy transfer (FRET)-based calcium biosensor TN-XXL. Using fluorescence spectroscopy of engineered tyrosines, we show that two of the four calcium binding EF-hands dominate the FRET output of TN-XXL and that local conformational changes of these hands match the kinetics of FRET change. Using small-angle x-ray scattering and NMR spectroscopy, we show that TN-XXL changes from a flexible elongated to a rigid globular shape upon binding calcium, thus resulting in FRET signal output. Furthermore, we compare calcium titrations using fluorescence lifetime spectroscopy with the ratiometric approach and investigate potential non-FRET effects that may affect the fluorophores. Thus, our data characterize the biophysics of TN-XXL in detail and may form a basis for further rational engineering of FRET-based biosensors.

Geiger, Anselm; Russo, Luigi; Gensch, Thomas; Thestrup, Thomas; Becker, Stefan; Hopfner, Karl-Peter; Griesinger, Christian; Witte, Gregor; Griesbeck, Oliver

2012-01-01

248

Isolation of Salmonella typhimurium Tn5 Mutants Defective for Survival on Egg Shell Surface Using Transposon Footprinting  

Microsoft Academic Search

The objective of this study was to screen the genome of Salmonella typhimurium for genes potentially required for survival on egg shell surfaces using a novel transposon footprinting method based on a previously developed Tn5 mutagenesis technique. Since the amplified DNA fragment length should usually be unique for each mutant, the polymerase chain reaction products separated on an agarose gel

Y. M. Kwon; L. F. Kubena; D. J. Nisbet; S. C. Ricke

2003-01-01

249

Chromosomal Deletion Formation System Based on Tn5 Double Transposition: Use For Making Minimal Genomes and Essential Gene Analysis  

Microsoft Academic Search

In this communication, we describe the use of specialized transposons (Tn5 derivatives) to create deletions in the Escherichia coli K-12 chromosome. These transposons are essentially rearranged composite transposons that have been assembled to promote the use of the internal transposon ends, resulting in intramolecular transposition events. Two similar transposons were developed. The first deletion transposon was utilized to create a

Igor Y. Goryshin; Todd A. Naumann; Jennifer Apodaca; William S. Reznikoff

2007-01-01

250

Relationship between summarizing chemical parameters like AOX, TOC, TN b , and toxicity tests for effluents from the chemical production  

Microsoft Academic Search

Therefore this study was undertaken to investigate, whether there are correlations between summarizing parameters, which potentially indicate hazardous water components, like AOX (adsorbable organic halogen), TOC (total organic carbon) and TN b (total bound nitrogen) and biological effects, observed from different bioassays conducted in the laboratory. The toxic effects were investigated on luminescent bacteria, microcrustaceans and algae in accordance with

G. Gellert

2000-01-01

251

76 FR 33401 - Environmental Impact Statement: Will and Kankakee Counties, Illinois and Lake County, IN  

Federal Register 2010, 2011, 2012, 2013

...Statement: Will and Kankakee Counties, Illinois and Lake County, IN AGENCY: Federal...Project in Will and Kankakee Counties, Illinois and Lake County, Indiana. FOR FURTHER...Executive Park Drive, Springfield, Illinois 62703, Phone: (217)...

2011-06-08

252

Digital Atlas of Texas Counties  

NSDL National Science Digital Library

Created by the Center for Geosptial Technology at Texas Tech University, this digital atlas is a fantastic find. It brings together information about all of Texas's counties, including satellite relief maps and data sets about the roads, rivers, lakes, and other features in each area. Visitors can use the interactive map to click on a county of interest, or use the drop down menu to select a region. Once users select a county, they can download information about it for future use. The site also contains a number of Featured Links to resources from the U.S. Census Bureau, the Texas Association of Counties, and several tourism agencies.

253

A rapid and simple method for the isolation of mutant variants regulating tissue-specific expression of the TnI gene through drug selection  

SciTech Connect

TnINEO fusion gene was constructed by fusing 3.4-kbp of quail TnI genomic DNA sequences spanning the promoter to exon 5 and a neo gene in frame. A myoblast cell line was established after transfection of pTnINEO. Since this cell line was passaged several times, a high frequency of neomycin (G418) sensitivity conversion was detected. Two drug-resistant variants were analyzed through genomic Southern blot and S1 nuclease protection assay. One variant has a mutation(s) in the regulatory element that activated the dormant TnI promoter-enhancer in myoblast, and the other has shown the geonomic rearrangement. This result presented the possibility of isolating factor(s) that activate the muscle-specific TnI promoter simply by screening drug-resistant cells having appropriate mutations. 12 refs., 4 fig.

Lee, Youngwon; Kim, Myoung Hee [Korea Research Institute of Bioscience and Biotechnology, Taejon (Korea, Republic of); Emerson, C.P. Jr. [Fox Chase Cancer Center, Philadelphia, PA (United States)

1995-12-01

254

Equity and County College Financing.  

ERIC Educational Resources Information Center

|An analysis by the Commission on Financial Postsecondary Education of the system by which New Jersey supports county colleges revealed disparities and inequities among the New Jersey counties in wealth and effort per full-time equivalent student, and current state financing practices that aggravate this problem. In response, alternative state…

New Jersey State Commission on Financing Postsecondary Education, Trenton.

255

Frederick County Community Perception Survey.  

ERIC Educational Resources Information Center

In 1997, Frederick Community College (FCC) in Maryland conducted a telephone survey of a random sample of 466 Frederick County residents to identify their perceptions of the college. In particular, the survey examined Frederick County residents' image of FCC, level of awareness of services and programs offered by FCC, and the types of services…

Frederick Community Coll., MD.

256

Bradley County Historic Site Survey.  

National Technical Information Service (NTIS)

The report is a study of areas and sites within Bradley County which have potential for historic preservation. The sites selected are considered to have substantial background and have been involved in the cultural development of the county to rate histor...

1973-01-01

257

POWER IN A RURAL COUNTY  

Microsoft Academic Search

To more fully understand power structures in rural settings, this article investigates the power structure of an economically depressed rural county in southwestern Pennsylvania. The power structure must be viewed in the context of the economic hard times that the county has confronted. In this regard, there has been a decline in the number of local economic notables over the

WILLIAM BEAVER; ERIC COHEN

2004-01-01

258

Galveston County Birth Data, 2002  

Microsoft Academic Search

The total of 3,872 county resident births in 2002 was 152 (4%) more than in 2001. Texas births increased 2%. By ethnicity, Galveston County increases were 11% among black residents, 5% among Hispanics, and 2% among all other residents. \\

Warren J. Holland; Chief Operating Officer

259

Development of a Colloid Sampling Technique for Suboxic Groundwaters in Memphis, TN  

NASA Astrophysics Data System (ADS)

Techniques for colloid isolation have been relatively well documented to date, but have been conducted primarily under oxic conditions. New methods to resolve sub- or anoxic conditions must be developed to facilitate further advances in understanding colloidal behaviors across all environments. Suboxic (diss. O2 ~ 45-57 ?g/L) conditions, characteristic of groundwater from the Memphis Aquifer (Memphis, TN), have been observed to generate iron precipitates via oxidation upon exposure to the atmosphere. It is believed this will confound any attempts toward the large volume (>1000 L) sampling of the colloid phase of groundwater flow using cross-flow filtration (CFF) methods. Integration of a high-pressure (40 psi) reservoir to the recycle loop design in CFF is proposed to concentrate colloids from groundwater samples. The results represent the testing of the modified Pellicon ultrafilter design, including a robust recharacterization of collection efficiency and establishment of the system's capacity to preserve in-situ oxygen concentrations sampled from non-oxic subsurface environments.

Todd, V. M.; Gentry, R. W.; McCarthy, J.; Ku, T.

2005-12-01

260

Alterations in peptidoglycan precursors and vancomycin susceptibility in Tn917 insertion mutants of Enterococcus faecalis 221.  

PubMed Central

Derivatives of the highly vancomycin-resistant Enterococcus faecalis strain 221 (MIC, 1,024 micrograms/ml) harboring Tn917 insertions in vanR, vanH, and vanA were compared with the parent strain and the susceptible plasmid-free strain JH2-2 (MIC, 2 micrograms/ml). Cytoplasmic pools of UDP-N-acetyl-muramyl-peptide precursors of strain 221 contained the depsipeptide-terminating precursor as well as elevated levels of both the tripeptide and tetrapeptide precursors. Insertional inactivation of vanR resulted in the loss of carboxypeptidase activity, full susceptibility to vancomycin, and precursor pools similar to those of JH2-2. For the vanA insertional mutant the MBC of vancomycin was fourfold higher than that for JH2-2, and the mutant had increased levels of tripeptide and tetrapeptide precursors compared with those for JH2-2. The vanH insertional mutant showed elevated levels of these precursors, as well as a small amount of depsipeptide, and both the MIC and the MBC of vancomycin were increased compared with those for JH2-2. These findings suggest that DD-carboxypeptidase activity, under the control of vanR, results in increased pools of both tripeptide and tetrapeptide precursors, which may contribute to survival in the presence of vancomycin.

Handwerger, S

1994-01-01

261

Public Health Assessment for White Oak Creek Radionuclide Releases, Oak Ridge Reservation (USDOE), Oak Ridge, Roane County, Tennessee, August 3, 2006. EPA Facility ID: TN1890090003.  

National Technical Information Service (NTIS)

This public health assessment evaluates the releases of radionuclides to the Clinch River (and the Lower Watts Bar Reservoir, or LWBR) from the ORR via White Oak Creek, assesses past, current, and future exposure to radionuclide releases for people who us...

2006-01-01

262

Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization.  

PubMed

We examined the release of cardiac troponin T (cTnT) and I (cTnI) into the blood of patients after acute myocardial infarction (AMI). Three postAMI serum samples were applied in separate analytical runs onto a calibrated gel filtration column (Sephacryl S-200), and the proteins were separated by molecular weight. Using commercial cTnT and cTnI assays measured on collected fractions, we found that troponin was released into blood as a ternary complex of cTnT-I-C, a binary complex of cTnI-C, and free cTnT, with no free cTnI within the limits of the analytical methodologies. The serum samples were also examined after incubation with EDTA and heparin. EDTA broke up troponin complexes into individual subunits, whereas heparin had no effect on the assays tested. We added free cTnC subunits to 24 AMI serum samples and found no marked increase in the total cTnI concentrations, using an immunoassay that gave higher values for the cTnI-C complex than free cTnI. To characterize the cross-reactivity of cTnT and cTnI assays, purified troponin standards in nine different forms were prepared, added to serum and plasma pools, and tested in nine quantitative commercial and pre-market assays for cTnI and one approved assay for cTnT. All nine cTnI assays recognized each of the troponin I forms (complexed and free). In five of these assays, the relative responses for cTnI were nearly equimolar. For the remainder, the response was substantially greater for complexed cTnI than for free cTnI. Moreover, there was a substantial difference in the absolute concentration of results between cTnI assays. The commercial cTnT assay recognized binary and ternary complexes of troponin on a near equimolar basis. We conclude that all assays are useful for detection of cardiac injury. However, there are differences in absolute cTnI results due to a lack of mass standardization and heterogeneity in the cross-reactivities of antibodies to various troponin I forms. PMID:9625043

Wu, A H; Feng, Y J; Moore, R; Apple, F S; McPherson, P H; Buechler, K F; Bodor, G

1998-06-01

263

King County Snapshots  

NSDL National Science Digital Library

This fine photographic archive serves as both a great repository of visual historical documentation of the King County area (which includes Seattle) in the state of Washington, as a good example of a collaborative partnership between various organizations. The partnership includes bringing together the visual collections of ten small historical organizations in tandem with the University of Washington and Seattle's Museum of History and Industry. Visitors can read about the working relationship between the organizations, view training materials from three workshops (such as one on image selection), and examine a list of online resources on subjects such as scanning digital images and metadata guidelines. Lest one forget the extensive visual materials (over 12,000 items as of the last count), visitors may search across each distinct collection, or elect to browse through each one individually. To get visitors started with using the archive, a number of sample searches are provided here as well.

264

Hydrology of Polk County, Florida  

USGS Publications Warehouse

Local water managers usually rely on information produced at the State and regional scale to make water-resource management decisions. Current assessments of hydrologic and water-quality conditions in Polk County, Florida, commonly end at the boundaries of two water management districts (South Florida Water Management District and the Southwest Florida Water Management District), which makes it difficult for managers to determine conditions throughout the county. The last comprehensive water-resources assessment of Polk County was published almost 40 years ago. To address the need for current countywide information, the U.S. Geological Survey began a 3?-year study in 2002 to update information about hydrologic and water-quality conditions in Polk County and identify changes that have occurred. Ground-water use in Polk County has decreased substantially since 1965. In 1965, total ground-water withdrawals in the county were about 350 million gallons per day. In 2002, withdrawals totaled about 285 million gallons per day, of which nearly 95 percent was from the Floridan aquifer system. Water-conservation practices mainly related to the phosphate-mining industry as well as the decrease in the number of mines in operation in Polk County have reduced total water use by about 65 million gallons per day since 1965. Polk County is underlain by three principal hydrogeologic units. The uppermost water-bearing unit is the surficial aquifer system, which is unconfined and composed primarily of clastic deposits. The surficial aquifer system is underlain by the intermediate confining unit, which grades into the intermediate aquifer system and consists of up to two water-bearing zones composed of interbedded clastic and carbonate rocks. The lowermost hydrogeologic unit is the Floridan aquifer system. The Floridan aquifer system, a thick sequence of permeable limestone and dolostone, consists of the Upper Floridan aquifer, a middle semiconfining unit, a middle confining unit, and the Lower Floridan aquifer. The Upper Floridan aquifer provides most of the water required to meet demand in Polk County. Data from about 300 geophysical and geologic logs were used to construct hydrogeologic maps showing the tops and thicknesses of the aquifers and confining units within Polk County. Thickness of the surficial aquifer system ranges from several feet thick or less in the extreme northwestern part of the county and along parts of the Peace River south of Bartow to more than 200 feet along the southern part of the Lake Wales Ridge in eastern Polk County. Thickness of the intermediate aquifer system/intermediate confining unit is highly variable throughout the county because of past erosional processes and sinkhole formation. Thickness of the unit ranges from less than 25 feet in the extreme northwestern part of the county to more than 300 feet in southwestern Polk County. The altitude of the top of the Upper Floridan aquifer in the county ranges from about 50 feet above National Geodetic Vertical Datum of 1929 (NGVD 29) in the northwestern part to more than 250 feet below NGVD 29 in the southern part. Water levels in the Upper Floridan aquifer fluctuate seasonally, increasing during the wet season (June through September) and decreasing during the rest of the year. Water levels in the Upper Floridan aquifer also can change from year to year, depending on such factors as pumpage and climatic variations. In the southwestern part of the county, fluctuations in water use related to phosphate mining have had a major impact on ground-water levels. Hydrographs of selected wells in southwestern Polk County show a general decline in water levels that ended in the mid-1970s. This water-level decline coincides with an increase in water use associated with phosphate mining. A substantial increase in water levels that began in the mid-1970s coincides with a period of decreasing water use in the county. Despite reductions in water use since 1970, howev

Spechler, Rick M.; Kroening, Sharon E.

2007-01-01

265

Bacteroides fragilis transfer factor Tn5520: the smallest bacterial mobilizable transposon containing single integrase and mobilization genes that function in Escherichia coli.  

PubMed

Many bacterial genera, including Bacteroides spp., harbor mobilizable transposons, a class of transfer factors that carry genes for conjugal DNA transfer and, in some cases, antibiotic resistance. Mobilizable transposons are capable of inserting into and mobilizing other, nontransferable plasmids and are implicated in the dissemination of antibiotic resistance. This paper presents the isolation and characterization of Tn5520, a new mobilizable transposon from Bacteroides fragilis LV23. At 4,692 bp, it is the smallest mobilizable transposon reported from any bacterial genus. Tn5520 was captured from B. fragilis LV23 by using the transfer-deficient shuttle vector pGAT400DeltaBglII. The termini of Tn5520 contain a 22-bp imperfect inverted repeat, and transposition does not result in a target site repeat. Tn5520 also demonstrates insertion site sequence preferences characterized by A-T-rich nucleotide sequences. Tn5520 has been sequenced in its entirety, and two large open reading frames whose predicted protein products exhibit strong sequence similarity to recombinase-integrase enzymes and mobilization proteins, respectively, have been identified. The transfer, mobilization, and transposition properties of Tn5520 have been studied, revealing that Tn5520 mobilizes plasmids in both B. fragilis and Escherichia coli at high frequency and also transposes in E. coli. PMID:10198023

Vedantam, G; Novicki, T J; Hecht, D W

1999-04-01

266

Bacteroides fragilis Transfer Factor Tn5520: the Smallest Bacterial Mobilizable Transposon Containing Single Integrase and Mobilization Genes That Function in Escherichia coli  

PubMed Central

Many bacterial genera, including Bacteroides spp., harbor mobilizable transposons, a class of transfer factors that carry genes for conjugal DNA transfer and, in some cases, antibiotic resistance. Mobilizable transposons are capable of inserting into and mobilizing other, nontransferable plasmids and are implicated in the dissemination of antibiotic resistance. This paper presents the isolation and characterization of Tn5520, a new mobilizable transposon from Bacteroides fragilis LV23. At 4,692 bp, it is the smallest mobilizable transposon reported from any bacterial genus. Tn5520 was captured from B. fragilis LV23 by using the transfer-deficient shuttle vector pGAT400?BglII. The termini of Tn5520 contain a 22-bp imperfect inverted repeat, and transposition does not result in a target site repeat. Tn5520 also demonstrates insertion site sequence preferences characterized by A-T-rich nucleotide sequences. Tn5520 has been sequenced in its entirety, and two large open reading frames whose predicted protein products exhibit strong sequence similarity to recombinase-integrase enzymes and mobilization proteins, respectively, have been identified. The transfer, mobilization, and transposition properties of Tn5520 have been studied, revealing that Tn5520 mobilizes plasmids in both B. fragilis and Escherichia coli at high frequency and also transposes in E. coli.

Vedantam, Gayatri; Novicki, Thomas J.; Hecht, David W.

1999-01-01

267

Genetic analysis of transcriptional activation and repression in the Tn21 mer operon. [Bacteria  

SciTech Connect

Transcription of the Tn21 mercury resistance operon (mer) is controlled by the toxic metal cation Hg(II). This control is mediated by the product of the merR gene, a 144-amino-acid protein which represses transcription of the structural genes (merTPCAD) in the absence of Hg(II) and activates transcription in the presence of Hg(II). We have used a mer-lac transcriptional fusion to obtain regulatory mutants in this metal-responsive system. Some mutants were defective in Hg(II)-induced activation while retaining repression function, others were defective in repression but not activation, and some had lost both functions. Mutations in three of the four cysteine residues of merR resulted in complete loss of Hg(II)-inducible activation but retention of the repressor function. Other lesions adjacent to or very near these cysteines exhibited severely reduced activation and also retained repressor function. There were two putative helix-turn-helix (HTH) domains in merR, and mutants in each had very different phenotypes. A partially dominant mutation in the more amino-terminal region of the two putative HTH regions resulted in loss of both activation and repression, consistent with a role for this region in DNA binding. Mutations in the more centrally located HTH region resulted only in loss of Hg(II)-induced activation. Lesions in the central and in the carboxy-terminal regions of merR exhibited both Hg(II)-independent and Hg(II)-dependent transcriptional activation. The sole cis-acting mutant obtained with this operon fusion strategy, a down-promoter mutation, lies in a highly conserved base in the -35 region of the merTPCAD promoter.

Ross, W.; Park, S.J.; Summers, A.O. (Univ. of Georgia, Athens (USA))

1989-07-01

268

Explosive Demolition of a Fire-Water Tower At East Tennessee Technology Park, Oak Ridge TN  

SciTech Connect

On June 17, 2006, the Department of Energy (DOE) successfully demolished a {approx}60 year old fire-water tower (K-1206-E), located at the East Tennessee Technology Park (ETTP) in Oak Ridge, TN, using strategically placed explosive charges. The subject demolition project was executed by MCM Management Corporation and Demolition Dynamics under the management of DoE's prime contractor Bechtel Jacobs Company LLC (BJC). The K-1206-E Fire Water Tower (Tower) supported the ETTP fire water protection system from the mid- 1950's until 1991. The 378,500-L (100,000-gallon) Tower, elevated 53-m (175-feet) above grade, was located in a grassy area within 152-m (500-feet) of several other occupied facilities. Electrical, control circuits and supply water servicing the Tower were deactivated in 2003. Free liquids and sludge were removed from the tank prior to demolition. Demolition of a facility employing explosive demolition at a federal site in the 'post-9/11 era' was a substantial challenge. The subject paper discusses: - the planning and coordination steps that were taken to successfully overcome the challenges prior to the demolition of the empty, deactivated Tower; - the method used for the engineered demolition of the Tower; and - the factors responsible for the successful execution of this demolition project. At least two previous attempts were made to demolish the Tower. In the first attempt, the execution of the project was deferred by the re-allocation of funds. In the subsequent attempt in 2004, the execution of this project was postponed due to concerns that an adjacent facility would have to shut down operations during the duration of mobilization and execution of the project and thereby incur potential financial losses. A total of 51 cubic meters (1,800 cubic feet) of demolition debris was generated, which was compliantly disposed of at a local landfill followed by site restoration.

Brooksbank, R.D.; Rood, M.S.; Amrit, S.K.; Harper, M.S.; Dypolt, D.J.; Brehse, Mike [Bechtel Jacobs Company LLC, P.O. Box 4699 Oak Ridge, TN 37931 (United States)

2008-01-15

269

Loss of catalase activity in Tn1545-induced mutants does not reduce growth of Listeria monocytogenes in vivo.  

PubMed Central

Two catalase-negative mutants of Listeria monocytogenes were obtained by chromosomal insertions of the conjugative transposon Tn1545. The loss of catalase activity did not reduce the level of virulence of these mutants in mice. Indeed, both mutants were capable of growing in host tissues at the same rate as the parental catalase-positive strain. These results favor the view that catalase does not play a critical role in the resistance of L. monocytogenes to macrophage killing.

Leblond-Francillard, M; Gaillard, J L; Berche, P

1989-01-01

270

Development of stable reporter system cloning luxCDABE genes into chromosome of Salmonella enterica serotypes using Tn7 transposon  

Microsoft Academic Search

BACKGROUND: Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent Salmonella enterica serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based

Kevin Howe; Attila Karsi; Pierre Germon; Robert W Wills; Mark L Lawrence; Richard H Bailey

2010-01-01

271

Targeting of T/Tn Antigens with a Plant Lectin to Kill Human Leukemia Cells by Photochemotherapy  

PubMed Central

Photochemotherapy is used both for solid tumors and in extracorporeal treatment of various hematologic disorders. Nevertheless, its development in oncology remains limited, because of the low selectivity of photosensitizers (PS) towards human tumor cells. To enhance PS efficiency, we recently covalently linked a porphyrin (TrMPyP) to a plant lectin (Morniga G), known to recognize with high affinity tumor-associated T and Tn antigens. The conjugation allowed a quick uptake of PS by Tn-positive Jurkat leukemia cells and efficient PS-induced phototoxicity. The present study was performed: (i) to evaluate the targeting potential of the conjugate towards tumor and normal cells and its phototoxicity on various leukemia cells, (ii) to investigate the mechanism of conjugate-mediated cell death. The conjugate: (i) strongly increased (×1000) the PS phototoxicity towards leukemic Jurkat T cells through an O-glycan-dependent process; (ii) specifically purged tumor cells from a 1?1 mixture of Jurkat leukemia (Tn-positive) and healthy (Tn-negative) lymphocytes, preserving the activation potential of healthy lymphocytes; (iii) was effective against various leukemic cell lines with distinct phenotypes, as well as fresh human primary acute and chronic lymphoid leukemia cells; (iv) induced mostly a caspase-independent cell death, which might be an advantage as tumor cells often resist caspase-dependent cell death. Altogether, the present observations suggest that conjugation with plant lectins can allow targeting of photosensitizers towards aberrant glycosylation of tumor cells, e.g. to purge leukemia cells from blood and to preserve the normal leukocytes in extracorporeal photochemotherapy.

Poiroux, Guillaume; Lafont, Elodie; Segui, Bruno; Van Damme, Els J. M.; Peumans, Willy J.; Bernadou, Jean; Levade, Thierry; Rouge, Pierre; Barre, Annick; Benoist, Herve

2011-01-01

272

First report of an OXA-23 carbapenemase-producing Acinetobacter baumannii clinical isolate related to Tn2006 in Spain.  

PubMed

A carbapenem-resistant Acinetobacter baumannii clinical isolate belonging to European clone II and sequence type 2 was recovered from a patient in the Son Espases hospital in Mallorca, Spain. Genetic analysis showed the presence of the bla(OXA-23) gene in association with the widely disseminated transposon Tn2006. This is the first reported identification of A. baumannii carrying bla(OXA-23) in Spain. PMID:23070166

Espinal, P; Macià, M D; Roca, I; Gato, E; Ruíz, E; Fernández-Cuenca, F; Oliver, A; Rodríguez-Baño, J; Bou, G; Tomás, M; Vila, J

2012-10-15

273

Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif  

Microsoft Academic Search

Bacterial DNA transposition is an important model system for studying DNA recombination events such as HIV-1 DNA integration and RAG-1-mediated V(D)J recombination. This communication focuses on the role of protein-phosphate contacts in manipulating DNA structure as a requirement for transposition catalysis. In particular, the participation of the nontransferred strand (NTS) 5' phosphate in Tn5 transposition strand transfer is analyzed. The

Vadim A. Klenchin; Agata Czyz; Igor Y. Goryshin; Richard Gradman; Scott Lovell; Ivan Rayment; William S. Reznikoff

2009-01-01

274

A high-performance short-channel bottom-contact OTFT and its application to AM-TN-LCD  

Microsoft Academic Search

We have demonstrated an organic thin-film-transistor (OTFT)-driven active-matrix twisted-nematic liquid crystal display (AM-TN-LCD) on a glass substrate, with a resolution of 160×120 pixels, 79 ppi. Substrate temperature was kept below the plastic-compatible temperature of 180°C throughout the fabrication process. In order to realize an OTFT-driven display with fine resolution, we employed short-channel bottom-contact (BC) pentacene OTFTs. It has been known

Kazumasa Nomoto; Nobukazu Hirai; Nobuhide Yoneya; Noriyuki Kawashima; Makoto Noda; Masaru Wada; Jiro Kasahara

2005-01-01

275

A new ?-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for ?-galactosidase activity of gastrointestinal bacteria  

Microsoft Academic Search

The ?-galactosidase gene, galA17, was cloned from Flavobacterium sp. TN17, a symbiotic bacterium isolated from the gut of Batocera horsfieldi larvae. The 2,205-bp full-length gene encodes a 734-residue polypeptide (GalA17) containing a putative 28-residue signal\\u000a peptide and a catalytic domain belonging to glycosyl hydrolase family 36 (GH 36). The deduced amino acid sequence of galA17 was most similar to a

Junpei Zhou; Pengjun Shi; Huoqing Huang; Yanan Cao; Kun Meng; Peilong Yang; Rui Zhang; Xiaoyan Chen; Bin Yao

2010-01-01

276

Tn 1 insertion mutagenesis in Escherichia coli K-12 using a temperature-sensitive mutant of plasmid RP4  

Microsoft Academic Search

A method for Tn1 insertion mutagenesis in Escherichia coli has been developed using pTH10, a mutant plasmid of RP4 temperature-sensitive for maintenance. The mutagenesis involves three steps. Firstly, from strains carrying pTH10 showing resistance to the antibiotics kanamycin, tetracycline, and ampicillin at 30° C but not at 42° C, clones are isolated resistant to kanamycin at 42° C. Such temperature-independent,

Shigeaki Harayama; Masataka Tsuda; Tetsuo Iino

1981-01-01

277

Proposed Rutherford County Airport, Rutherford County, North Carolina.  

National Technical Information Service (NTIS)

Rutherford County is now preparing a request for Federal funds under the Airport and Airway Development Act for the following development: proposes land acquisition (approximately 162 acres with relocation of three houses and two mobile homes for the Ruth...

1973-01-01

278

Analysis for prevalence and physical linkages amongst integrons, ISEcp1, ISCR1, Tn21 and Tn7 encountered in Escherichia coli strains from hospitalized and non-hospitalized patients in Kenya during a 19-year period (1992-2011)  

PubMed Central

Background We determined the prevalence and evidence for physical linkage amongst integrons, insertion sequences, Tn21 and Tn7 transposons in a collection of 1327 E. coli obtained over a 19-year period from patients in Kenya. Results The prevalence of class 1 integrons was 35%, class 2 integrons were detected in 3 isolates but no isolate contained a class 3 integron. Integron lacking the 3’-CS or those linked to sul3 gene or IS26 or those containing the ISCR1 were only detected in multidrug resistant (MDR) strains. The dfrAs were the most common cassettes and their prevalence was: - dfrA1(28%), dfrA12(20%), dfA17(9%), dfrA7(9%), and dfrA16(5%). The aadA were the second most abundant cassettes and their prevalence was: - aadA1(25%), aadA2(21%), and aadA5(14%). Other cassettes occurred in lower prevalence of below 5%. Prevalence of Tn21, ISEcp1, ISCR1 and IS26 was 22%, 10%, 15%, and 7% respectively. Majority of Tn21 containing integrons carried a complete set of transposition genes while class 2 integrons were borne on Tn7 transposon. The qnrA genes were detected in 34(3%) isolates while 19(1%) carried qnrB. All qnr genes were in MDR strains carrying integrons containing the ISCR1. Close to 88% of blaTEM-52 were linked to IS26 while???80% of blaCTX-Ms and blaCMYs were linked to ISEcp1. Only a few studies have identified a blaCTX-M-9 containing an ISEcp1 element as reported in this study. Multiple genetic elements, especially those borne on incIl, incFII, and incL/M plasmids, and their associated resistance genes were transferrable en bloc to E. coli strain J53 in mating experiments. Conclusions This is the first detailed study on the prevalence of selected elements implicated in evolution of resistance determinants in a large collection of clinical E. coli in Africa. Proliferation of such strains carrying multiple resistance elements is likely to compromise the use of affordable and available treatment options for majority of poor patients in Africa. There is therefore a need to monitor the spread of these highly resistant strains in developing countries through proper infection control and appropriate use of antimicrobials.

2013-01-01

279

Testing and analyses of the TN-24P PWR spent-fuel dry storage cask loaded with consolidated fuel  

SciTech Connect

A performance test of a Transnuclear, Inc. TN-24P storage cask configured for pressurized water reactor (PWR) spent fuel was performed. The work was performed by the Pacific Northwest Laboratory (PNL) and Idaho National Engineering Laboratory (INEL) for the US Department of Energy Office of Civilian Radioactive Waste Management (OCRWM) and the Electric Power Research Institute. The performance test consisted of loading the TN-24P cask with 24 canisters of consolidated PWR spent fuel from Virginia Power's Surry and Florida Power and Light's Turkey Point reactors. Cask surface and fuel canister guide tube temperatures were measured, as were cask surface gamma and neutron dose rates. Testing was performed with vacuum, nitrogen, and helium backfill environments in both vertical and horizontal cask orientations. Transnuclear, Inc., arranged to have a partially insulated run added to the end of the test to simulate impact limiters. Limited spent fuel integrity data were also obtained. From both heat transfer and shielding perspectives, the TN-24P cask with minor refinements can be effectively implemented at reactor sites and central storage facilities for safe storage of unconsolidated and consolidated spent fuel. 35 refs., 93 figs., 17 tabs.

McKinnon, M A; Michener, T E; Jensen, M F; Rodman, G R

1989-02-01

280

Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant.  

PubMed

Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5Tnp Transposome (Epicentre). Up to 1 x 10(6) mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. PMID:18060666

Rouws, Luc F M; Simões-Araújo, Jean L; Hemerly, Adriana S; Baldani, José I

2007-12-05

281

Construction of a Tn5-tagged mutant library of Xanthomonas oryzae pv. oryzicola as an invaluable resource for functional genomics.  

PubMed

To genome-widely mine pathogenesis-related genes of Xanthomonas oryzae pv. oryzicola (Xoc), which is the casual agent of bacterial leaf streak resulting in significant yield loss and poor quality in rice, a Tn5 transposon-mediated mutation library was generated. Twenty-five thousand transformants were produced by using Tn5 transposome, appropriately corresponding to 5 × ORF coverage of the genome, and inoculated into rice and tobacco, individually and respectively, for screening candidate virulence genes. Southern blot and thermal asymmetric interlaced polymerase chain reaction analysis of Tn5 insertion sites of randomly selected mutants suggested a random mode of transposition and a saturation library. Characterization of extracellular polysaccharides, extracellular protease activity, and pigment production of individual mutants in the growth media revealed that 11 mutants enhanced in growth, 12 reduced extracellular polysaccharide production, 12 lost extracellular protease activity completely or partially, and 21 were pigment deficient. In planta pathogenicity assays revealed 253 mutants reduced virulence in rice, but kept triggering hypersensitive response in tobacco; 49 lost the ability to elicit HR in tobacco and pathogenicity in rice; and 3 still induced hypersensitive response in tobacco, but lost pathogenicity in rice. The achieved mutant library of Xoc is of high-quality and nearly saturated and candidate virulence mutants provided a strong basis for functional genomics of Xoc. PMID:21046389

Zou, Hua-Song; Yuan, Liang; Guo, Wei; Li, Yu-Rong; Che, Yi-Zhou; Zou, Li-Fang; Chen, Gong-You

2010-11-04

282

65 FR 66489 - Irish Potatoes Grown in Modoc and Siskiyou Counties, California, and in all Counties in Oregon...  

Federal Register 2010, 2011, 2012, 2013

...Siskiyou Counties, California, and in all Counties in Oregon, Except Malheur County...marketing order. This rule also suspends all reporting and assessment collection requirements...Siskiyou Counties, California, and in all Counties in Oregon, except Malheur...

2000-11-06

283

65 FR 42275 - Irish Potatoes Grown in Modoc and Siskiyou Counties, California, and in all Counties in Oregon...  

Federal Register 2010, 2011, 2012, 2013

...Siskiyou Counties, California, and in all Counties in Oregon, except Malheur County...potato marketing order. It also suspends all reporting and assessment collection requirements...Siskiyou Counties, California, and in all Counties in Oregon, except Malheur...

2000-07-10

284

New Transposon Tn6133 in Methicillin-Resistant Staphylococcus aureus ST398 Contains vga(E), a Novel Streptogramin A, Pleuromutilin, and Lincosamide Resistance Gene?  

PubMed Central

A novel streptogramin A, pleuromutilin, and lincosamide resistance determinant, Vga(E), was identified in porcine methicillin-resistant Staphylococcus aureus (MRSA) ST398. The vga(E) gene encoded a 524-amino-acid protein belonging to the ABC transporter family. It was found on a multidrug resistance-conferring transposon, Tn6133, which was comprised of Tn554 with a stably integrated 4,787-bp DNA sequence harboring vga(E). Detection of Tn6133 in several porcine MRSA ST398 isolates and its ability to circularize suggest a potential for dissemination.

Schwendener, Sybille; Perreten, Vincent

2011-01-01

285

New transposon Tn6133 in methicillin-resistant Staphylococcus aureus ST398 contains vga(E), a novel streptogramin A, pleuromutilin, and lincosamide resistance gene.  

PubMed

A novel streptogramin A, pleuromutilin, and lincosamide resistance determinant, Vga(E), was identified in porcine methicillin-resistant Staphylococcus aureus (MRSA) ST398. The vga(E) gene encoded a 524-amino-acid protein belonging to the ABC transporter family. It was found on a multidrug resistance-conferring transposon, Tn6133, which was comprised of Tn554 with a stably integrated 4,787-bp DNA sequence harboring vga(E). Detection of Tn6133 in several porcine MRSA ST398 isolates and its ability to circularize suggest a potential for dissemination. PMID:21768510

Schwendener, Sybille; Perreten, Vincent

2011-07-18

286

Physical Inactivity Estimates, by County  

MedlinePLUS

... to... Añadir en... Favorites Delicious Digg Google Bookmarks Physical Inactivity Estimates, by County Staying active during leisure ... of obesity, type 2 diabetes and related diseases. Physical inactivity can lead to obesity and type 2 ...

287

Hospital Study, Porter County, Indiana.  

National Technical Information Service (NTIS)

Results of a study of hospital facilities and future needs in Porter County, Indiana are presented in this report of the Northwest Indiana Comprehensive Health Planning Council, Inc. In reviewing health needs, goals and priorities with providers and consu...

1972-01-01

288

Michigan County Histories and Atlases  

NSDL National Science Digital Library

How does one tell the story of a county? It's a tough task, but the staff members at the University of Michigan's Library system worked with a range of partners (including the Michigan Council of Library Directors) to make these county histories widely accessible. On this site, visitors can look over 428 digitized titles that tell the history of the Wolverine State. Interested parties can perform keyword searches across all of these volumes or just browse around at their leisure. First-time visitors might do well to look at the "Account of Kalamazoo County" from 1928 and the 1900 tome "An account of Southwest Michigan and Calhoun County." Additionally, the site contains business directories for a number of communities across the state. These documents are a great way to learn about the retail and other commercial activities going on from the 19th to the early 20th centuries. [KMG

2007-10-19

289

Community Analysis, Stafford County, Virginia.  

National Technical Information Service (NTIS)

This study of the present and future library needs of residents of Stafford County, Virginia, with emphasis on the North Stafford areas, includes a review of demographic statistics and recommendations for library services. The currently increasing populat...

1978-01-01

290

Fault Trace: Marin County, California  

NSDL National Science Digital Library

This photograph shows the trace of a fault (in trench phase) as it passes beneath a barn. The trace developed during the April 18, 1906 San Francisco Earthquake. The location is the Skinner Ranch, near Olema, Marin County, California.

291

Geothermal Development Plan: Pima County  

SciTech Connect

Pima County is located entirely within the Basin and Range physiographic province in which geothermal resources are known to occur. Continued growth as indicated by such factors as population growth, employment and income will require large amounts of energy. It is believed that geothermal energy could provide some of the energy that will be needed. Potential users of geothermal energy within the county are identified.

White, D.H.

1981-01-01

292

Atlas of Historical County Boundaries  

NSDL National Science Digital Library

So you'd like to know the boundaries of Washington's King County in the early 20th century? Or perhaps you'd like to know more about the shape of Nassau County in New York back in the 19th century? The Atlas of Historical County Boundaries can provide you such details. The project is housed at the William M. Scholl Center for American History and Culture at The Newberry Library in Chicago and it was completed in 2010. The Atlas includes such features as all boundary changes in states and countries, non-county areas, separate map or polygon files for every different county configuration, and other helpful features. Users can get started by clicking on different states of interest and just exploring as they see fit. It's also useful to read over the Using the Atlas area for additional assistance. Finally, visitors can also look at the Publications section for more information on the source material for these county boundaries.

293

Response of high-risk of recurrence/progression bladder tumours expressing sialyl-Tn and sialyl-6-T to BCG immunotherapy.  

PubMed

Background:High risk of recurrence/progression bladder tumours is treated with Bacillus Calmette-Guérin (BCG) immunotherapy after complete resection of the tumour. Approximately 75% of these tumours express the uncommon carbohydrate antigen sialyl-Tn (Tn), a surrogate biomarker of tumour aggressiveness. Such changes in the glycosylation of cell-surface proteins influence tumour microenvironment and immune responses that may modulate treatment outcome and the course of disease. The aim of this work is to determine the efficiency of BCG immunotherapy against tumours expressing sTn and sTn-related antigen sialyl-6-T (s6T).Methods:In a retrospective design, 94 tumours from patients treated with BCG were screened for sTn and s6T expression. In vitro studies were conducted to determine the interaction of BCG with high-grade bladder cancer cell line overexpressing sTn.Results:From the 94 cases evaluated, 36 had recurrence after BCG treatment (38.3%). Treatment outcome was influenced by age over 65 years (HR=2.668; (1.344-5.254); P=0.005), maintenance schedule (HR=0.480; (0.246-0.936); P=0.031) and multifocallity (HR=2.065; (1.033-4.126); P=0.040). sTn or s6T expression was associated with BCG response (P=0.024; P<0.0001) and with increased recurrence-free survival (P=0.001). Multivariate analyses showed that sTn and/or s6T were independent predictive markers of recurrence after BCG immunotherapy (HR=0.296; (0.148-0.594); P=0.001). In vitro studies demonstrated higher adhesion and internalisation of the bacillus to cells expressing sTn, promoting cell death.Conclusion:s6T is described for the first time in bladder tumours. Our data strongly suggest that BCG immunotherapy is efficient against sTn- and s6T-positive tumours. Furthermore, sTn and s6T expression are independent predictive markers of BCG treatment response and may be useful in the identification of patients who could benefit more from this immunotherapy. PMID:24064971

Lima, L; Severino, P F; Silva, M; Miranda, A; Tavares, A; Pereira, S; Fernandes, E; Cruz, R; Amaro, T; Reis, C A; Dall'olio, F; Amado, F; Videira, P A; Santos, L; Ferreira, J A

2013-09-24

294

Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation.  

PubMed

Tn5520 is the smallest known bacterial mobilizable transposon and was isolated from an antibiotic resistant Bacteroides fragilis clinical isolate. When a conjugation apparatus is provided in trans, Tn5520 is mobilized (transferred) efficiently within, and from, both Bacteroides spp. and Escherichia coli. Only two genes are present on Tn5520; one encodes an integrase, and the other a multifunctional mobilization (Mob) protein BmpH. BmpH is essential for Tn5520 mobility. The focus of this study was to identify the Tn5520 origin of conjugative transfer (oriT) and to study BmpH-oriT binding. We delimited the functional Tn5520 oriT to a 71 bp sequence upstream of the bmpH gene. A plasmid vector harbouring this minimal 71 bp oriT was mobilized at the same frequency as that of intact Tn5520. The minimal oriT contains one 17 bp inverted repeat (IR) sequence. We constructed and tested multiple IR mutants and showed that the IR was essential in its entirety for mobilization. A nick site sequence (5'-GCTAC-3') was also identified within the minimal oriT; this sequence resembled nick sites found in plasmids of Gram positive origin. We further showed that mutation of a highly conserved GC dinucleotide in the nick site sequence completely abolished mobilization. We also purified BmpH and showed that it specifically bound a Tn5520 oriT fragment in electrophoretic mobility shift assays. We also identified non-nick site sequences within the minimal oriT that were essential for mobilization. We hypothesize that transposon-based single Mob protein systems may contribute to efficient gene dissemination from Bacteroides spp., because fewer DNA processing proteins are required for relaxosome formation. PMID:16359335

Vedantam, Gayatri; Knopf, Sarah; Hecht, David W

2006-01-01

295

The conjugative transposon Tn 925 : enhancement of conjugal transfer by tetracycline in Enterococcus faecalis and mobilization of chromosomal genes in Bacillus subtilis and E. faecalis  

Microsoft Academic Search

The effects of tetracycline on transfer of the conjugative, tetracycline-resistance transposon, Tn925, as well as the ability of the transposon to promote the transfer of chromosomal genes was examined in Enterococcus faecalis and Bacillus subtilis. To test for chromosomal transfer, multiply-marked strains of each organism, each carrying a single chromosomal copy of Tn925, were mated on filters with suitable recipient

Olga R. Torres; Ruth Z. KormanZ; Stanley A. Zahler; Gary M. Dunny

1991-01-01

296

A hisT::Tn5 mutation affects production of microcins B17, C7, and H47 and colicin V.  

PubMed

A Tn5 insertion decreasing the production of microcin B17 was mapped to 50.2 min on the Escherichia coli chromosome map. Sequence analysis showed that the insertion disrupted hisT, the gene encoding pseudouridine synthase I, a tRNA-modifying enzyme. hisT::Tn5 mutant cells were also shown to be defective for the production of other antibiotic peptides, such as microcin C7, microcin H47, and colicin V. PMID:1657874

Rodríguez-Sáinz, M C; Hernández-Chico, C; Moreno, F

1991-11-01

297

Use of the Centaur TnI-Ultra Assay for Detection of Myocardial Infarction and Adverse Events in Patients Presenting With Symptoms Suggestive of Acute Coronary Syndrome  

Microsoft Academic Search

BACKGROUND: We determined the diagnostic accuracy of the Advia Centaur TnI-Ultra assay for detecting myocardial infarction (MI) and assessing risk of ad- verseeventsinpatientspresentingwithischemicsymp- toms suggestive of acute coronary syndrome. METHODS: We measured cardiac troponin I (cTnI) on admission and 6-24 h after admission (follow-up) in plasma specimens from 371 consecutive patients. The end point was the first of cardiac event

Fred S. Apple; Stephen W. Smith; Lesly A. Pearce; Ranka Ler; MaryAnn M. Murakami

298

Characterization of the gene for the Fe-protein of the vanadium dependent alternative nitrogenase of Azotobacter vinelandii and construction of a Tn 5 mutant  

Microsoft Academic Search

A sequence homologous to the conventional nifH gene has been cloned from a different region of the Azotobacter vinelandii genome. Tn5 insertions were obtained in this clone and the mutagenized plasmid was used for marker exchange with A. vinelandii strain CA12 (?nifHDK) to obtain Tn5 mutants. These mutants exhibited a Nif- phenotype in the presence of vanadium, unlike CA12 which

Ramesh Raina; M. Amarender Reddy; Debabrota Ghosal; H. K. Das

1988-01-01

299

Regional Specificity of Illegitimate Recombination by the Translocatable Amplicillin-Resistance Element Tn1 in the Genome of Phage P22  

PubMed Central

Insertions of the translocatable ampicillin-resistance element Tn1 were selected in the genome of the temperate Salmonella phage P22 by growing the phage on hosts carrying the resistance plasmid RP4. Insertions of Tn1 into phage P22 are rare (10-10 per phage) and nonrandomly distributed in the P22 genome. They are found mainly in the vicinity of the P22 ant gene. Insertions within the ant gene are found at many (at least 15) genetically separable sites, are found equally frequently in both orientations and cause irreversible loss of gene function. Some insertions in ant appear to be associated with an adjecent deletion.—Prophage deletions were derived from P22::Tn1 phages by two methods. Low multiplicity transductants have nonrandomly distributed endpoints. One end is at or very near the site of the Tn1 insertion, and the other is in the vicinity of gene 12; however, there are many genetically distinguishable endpoints within gene 12. Prophage deletions selected as survivors of induction of a P22Ap mnt-ts lysogen have similarly nonrandom endpoints, with the Tn1-distal end frequently near the ant gene, as well as gene 12. Physical analysis of several prophage deletions suggests that the Tn1 is intact to the resolution of DNA electron microscopy and that the deletions begin at the end of the Tn1 insertion.—These results suggest that illegitimate recombination associated with Tn1 shows regional specificity (i.e., preference for some large areas of the P22 genome over other areas), but that within these regions is quite nonspecific.

Weinstock, George M.; Susskind, Miriam M.; Botstein, David

1979-01-01

300

Geothermal development plan: Yuma County  

SciTech Connect

The Yuma County Area Development Plan evaluated the county-wide market potential for utilizing geothermal energy. The study identified four potential geothermal resource areas with temperatures less than 90/sup 0/C (194/sup 0/F), and in addition, two areas are inferred to contain geothermal resources with intermediate (90/sup 0/C to 150/sup 0/C, 194/sup 0/F to 300/sup 0/F) temperature potential. The resource areas are isolated, although one resource area is located near Yuma, Arizona. One resource site is inferred to contain a hot dry rock resource. Anticipated population growth in the county is expected to be 2 percent per year over the next 40 years. The primary employment sector is agriculture, though some light industry is located in the county. Water supplies are found to be adequate to support future growth without advese affect on agriculture. Six firms were found in Yuma County which may be able to utilize geothermal energy for process heat needs. In addition, several agricultural processors were found, concentrated in citrus processing and livestock raising. Geothermal energy utilization projections suggest that by the year 2000, geothermal energy may economically provide the energy equivalent of 53,000 barrels of oil per year to the industrial sector if developed privately. Geothermal utilization projections increase to 132,000 barrels of oil per year by 2000 if a municipal utility developed the resource.

White, D.H.; Goldstone, L.A.

1982-08-01

301

Cardiac Myosin Heavy Chain Isoform Exchange Alters the Phenotype of cTnT-Related Cardiomyopathies in Mouse Hearts  

PubMed Central

Familial Hypertrophic Cardiomyopathy, FHC, is a clinically heterogeneous, autosomal-dominant disease of the cardiac sarcomere leading to extensive remodeling at both the whole heart and molecular levels. The remodeling patterns are mutation-specific, a finding that extends to the level of single amino acid substitutions at the same peptide residue. Here we utilize two well-characterized transgenic FHC mouse models carrying independent amino acid substitutions in the TM-binding region of cardiac troponin T (cTnT) at residue 92. R92Q and R92L cTnT domains have mutation-specific average peptide conformation and dynamics sufficient to alter thin filament flexibility and cross-bridge formation and R92 mutant myocytes demonstrate mutation-specific temporal molecular remodeling of Ca2+ kinetics and impaired cardiac contractility and relaxation. To determine if a greater economy of contraction at the crossbridge level would rescue the mechanical defects caused by the R92 cTnT mutations, we replaced the endogenous murine ?-myosin heavy chain (MyHC) with the ?-MyHC isoform. While ?-MyHC replacement rescued the systolic dysfunction in R92Q mice, it failed to rescue the defects in diastolic function common to FHC-associated R92 mutations. Surprisingly, a significant component of the whole heart and molecular contractile improvement in the R92Q mice was due to improvements in Ca2+ homeostasis including SR uptake, [Ca2+]i amplitude and phospholamban phosphorylation. Our data demonstrate that while genetically altering the myosin composition of the heart bearing a thin filament FHC mutation is sufficient to improve contractility, diastolic performance is refractory despite improved Ca2+ kinetics. These data reveal a previously unrecognized role for MyHC isoforms with respect to Ca2+ homeostasis in the setting of cardiomyopathic remodeling and demonstrate the overall dominance of the thin filament mutation in determining the degree of diastolic impairment at the myofilament level.

Rice, Ron; Guinto, Pia; Dowell-Martino, Candice; He, Huamei; Hoyer, Kirsten; Krenz, Maike; Robbins, Jeffrey; Ingwall, Joanne S.; Tardiff, Jil C.

2010-01-01

302

Elution of Nitrate at the NABIR Field Research Center, Oak Ridge Reservation, Oak Ridge, TN  

NASA Astrophysics Data System (ADS)

As part of a bioremediation project for the in situ bioreduction of uranium at the Department of Energy Natural and Accelerated Bioremediation Research (NABIR) Field Research Center (FRC) in Oak Ridge, TN, aquifer and groundwater conditioning is required before conducting the remediation experiment. One step includes flushing of the aquifer with pH-adjusted fresh water in order to remove extremely high concentrations of nitrate, calcium, and aluminum that would interfere with in situ bioreduction. The elution of nitrate from the test zone was used as an inverse tracer to discern contaminant transport pathways and model parameters. Concentration time series data augmented pressure tests, a bromide tracer study, and electromagnetic borehole flowmeter (EBF) measurements. The aquifer at the FRC is a fractured shale with strike of about 1.5 degrees north of west, and dip of about 30 degrees to the southwest, as inferred from area observations and EBF logging. A network of injection and extraction wells are aligned along strike, while a separate network of observation wells with multiple screen intervals (MLS wells) are oriented along dip at the midpoint of the injection/extraction well network. Flow generally occurs along strike in fractures associated with bedding planes, however other lesser fracture networks provide communication between the major fracture sets. Previous data have indicated a high hydraulic conductivity zone, approximately 10-50 cm thick located at a depth of about 12 m along the centerline of the injection/extraction well network. Above the major flow zone, the matrix weathers to saprolite, decreasing hydraulic conductivity. The elution tracer test was conducted by injecting clean, acidified tap water in the farthest upgradient injection well, and extracting at half the injection rate from the farthest downgradient extraction well. This flow ratio was chosen to produce flushing focused on a small cell of the aquifer which will later be used as an in situ bioreactor. Nitrate concentrations were measured frequently over 100 hours. Concentration time series indicate that flushing occurred predominantly in the previously identified high conductivity zone at 12 m depth. The strongest, most rapid nitrate concentration decrease was observed at a similar depth in each MLS well, which is consistent with the depth at which injection and extraction occurred. A decrease in downdip, nitrate concentration extended to 15m depth, whereas at the centerline and updip, little nitrate concentration reduction occurred below 13.75 m depth. This asymmetry is consistent with the competing contributions of bedding plane fractures and weathering depths. In the future, time series crosshole seismic and ground penetrating radar collected by Lawrence Berkeley National Laboratory, and borehole electrical conductivity measurements collected by Oak Ridge National Laboratory will be integrated with the concentration, pressure, and EBF data to obtain a comprehensive interpretation of this test.

Fienen, M. N.; Criddle, C. S.; Jardine, P. M.; Kitanidis, P. K.; Mehlhorn, T. L.; Watson, D. B.; Wu, W.

2003-12-01

303

Superfund Record of Decision (EPA Region 4): American Creosote Works, TN. (First Remedial Action), December 1988  

SciTech Connect

The American Creosote Works (ACW) site is located immediately southwest of Jackson, in central Madison County, Tennessee. ACW conducted wood-preserving operations using both creosote and PCP from the early 1930s until December 1981. Untreated process waste water and potential contaminated storm water runoff were discharged directly into Central Creek until 1973, at which time a levee was constructed to retain surface water runoff. The soil borrow pits used for the levee construction became sludge storage lagoons. A waste water treatment system was installed onsite during 1974 and 1975 and operated until 1981. The selected remedial action for the site includes consolidation and incineration of sludges in the vicinity of the buildings and tanks; on- or offsite incineration of the oils and sludges from the tanks; treatment of tanked process liquids onsite using a sand filter, filter press, and carbon adsorption unit, followed by discharge to a surface stream; decontamination and offsite disposal of site structures; construction of a flood-protection dike; deed restrictions and site fencing; and site stabilization including monitoring onsite water levels behind the dikes and pumping, treating (as needed), and discharging impounded water pending a final remedy.

Not Available

1989-01-05

304

Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis.  

PubMed

Twelve independent Tn10 insertion mutants of Escherichia coli K12 were isolated that were defective in the synthesis of enterobacterial common antigen (ECA). The mutants were identified by screening a random pool of Tn10 insertion mutants for their ECA phenotype using a colony-immunoblot assay. All 12 of the Tn10 insertion mutants were found to be located in the chromosomal region of the rff-rfe genes. Four of the Tn10 insertions were in rfe genes while the remaining eight Tn10 insertions were in rff genes. All of the rfe::Tn10 insertion mutants were defective in the synthesis of GlcNAc-pyrophosphorylundecaprenol (C55-PP-GlcNAc, lipid I), the first lipid-linked intermediate involved in ECA synthesis. Biochemical characterization of the rff::Tn10 insertion mutants revealed that they were defective in various steps of ECA synthesis subsequent to the synthesis of lipid I. These defects included: (i) the inability to synthesize UDP-ManNAcA due to Tn10 insertions in the structural genes for UDP-GlcNAc-2-epimerase (rffE) and UDP-ManNAcA (N-acetyl-D-mannosaminuronic acid) dehydrogenase (rffD), (ii) defects in the synthesis of C55-GlcNAc-ManNAcA (lipid II) due to insertion of transposon Tn10 in the structural gene for the UDP-ManNAcA transferase (rffM), (iii) the inability to synthesize TDP-Fuc4NAc (4-acetamido-4,6-dideoxy-D-galactose) due to Tn10 insertions in the structural gene for the transaminase that catalyzes the conversion of TDP-4-keto-6-deoxy-D-glucose to TDP-4-amino-4,6-dideoxy-D-galactose (rffA), and (iv) defects in steps subsequent to the synthesis of C55-GlcNAc-ManNAcA-Fuc4NAc (lipid III). In addition, a re-examination of a mutant possessing the rff-726 lesion revealed that it was defective in the synthesis of lipid III due to a defect in the structural gene for the Fuc4NAc transferase (rffT). PMID:2166030

Meier-Dieter, U; Starman, R; Barr, K; Mayer, H; Rick, P D

1990-08-15

305

Geothermal development plan: Maricopa county  

SciTech Connect

Maricopa county is the area of Arizona receiving top priority since it contains over half of the state's population. The county is located entirely within the Basin and Range physiographic region in which geothermal resources are known to occur. Several approaches were taken to match potential users to geothermal resources. One approach involved matching some of the largest facilities in the county to nearby geothermal resources. Other approaches involved identifying industrial processes whose heat requirements are less than the average assessed geothermal reservoir temperature of 110/sup 0/C (230/sup 0/F). Since many of the industries are located on or near geothermal resources, geothermal energy potentially could be adapted to many industrial processes.

White, D.H.

1981-01-01

306

Proposed Spink County Comprehensive Zoning Plan, Spink County, South Dakota.  

National Technical Information Service (NTIS)

A zoning plan is imperative to the orderly and economically sound growth of any county. It is therefore urged that great care and deliberation be taken in the efforts of implementing the proposed plan. Portions of the plan are designed to assist those per...

1973-01-01

307

Re-visiting the endogenous capacity for recombinant glycoprotein sialylation by baculovirus-infected Tn-4h and DpN1 cells  

PubMed Central

It was previously reported that Tn-4h and DpN1 cells have the endogenous capacity to efficiently sialylate secreted alkaline phosphatase (SEAP) when infected with a baculovirus expression vector. In contrast, it has been found that lepidopteran insect cell lines that are more widely used as hosts for baculovirus vectors typically fail to sialylate SEAP and other recombinant glycoproteins. Thus, the N-glycan processing capabilities of Tn-4h and DpN1 cells are of potential interest to investigators using the baculovirus expression system for recombinant glycoprotein production. In this study, we experimentally re-assessed the ability of Tn-4h and DpN1 cells to sialylate SEAP with Sf9 and glyco-engineered Sf9 cells (SfSWT-1) as negative and positive controls, respectively. Our results showed that the SEAP purified from SfSWT-1 cells was strongly sialylated and initially indicated that the SEAP purified from Tn-4h cells was weakly sialylated. However, further analyses suggested that the SEAP produced by Tn-4h cells only appeared to be sialylated because it was contaminated with an electrophoretically indistinguishable sialoglycoprotein derived from fetal bovine serum. We subsequently expressed, purified, and analyzed a second recombinant glycoprotein (GST-SfManI) from all four cell lines and found that only the SfSWT-1 cells were able to detectably sialylate this product. Together, these results showed that neither Tn-4h nor DpN1 cells efficiently sialylated SEAP or GST-SfManI when infected by baculovirus expression vectors. Furthermore, they suggested that previous reports of efficient SEAP sialylation by Tn-4h and DpN1 cells probably reflect contamination with a sialylated, co-migrating glycoprotein, perhaps bovine fetuin, derived from the serum used in the insect cell growth medium.

Hillar, Alexander; Jarvis, Donald L

2010-01-01

308

Transcription from Fusion Promoters Generated during Transposition of Transposon Tn4652 Is Positively Affected by Integration Host Factor in Pseudomonas putida  

PubMed Central

We have previously shown that both ends of the Tn3 family transposon Tn4652 contain integration host factor (IHF) binding sites and that IHF positively regulates expression of the Tn4652 transposase gene tnpA in Pseudomonas putida (R. Hõrak, and M. Kivisaar, J. Bacteriol. 180:2822–2829, 1998). Tn4652 can activate silent genes by creating fusion promoters during the transposition. The promoters are created as fusions between the ?35 hexamer provided by the terminal inverted repeats of Tn4652 and the ?10 hexamers in the target DNA. Two fusion promoters, PRA1 and PLA1, that contain sequences of the right and left termini of Tn4652, respectively, were chosen for the study of mechanisms of transcription activation. Gel mobility shift analysis using crude extracts from P. putida cells allowed us to detect specific binding of P. putida IHF to the ends of the transposon Tn4652. We found that the rate of transcription from the fusion promoter PRA1 is enhanced by IHF. Notably, the positive effect of IHF on transcription from the promoter PRA1 appeared only when cells of P. putida reached the stationary growth phase. We speculate that the intracellular concentration of IHF might be critical for the in vivo effect of IHF on transcription from the fusion promoters in P. putida. In the case of PLA1, the mechanism of transcription modulation by IHF is different than that observed for PRA1. Our results demonstrate that transcription of neighboring genes from outwardly directed promoters at the ends of a mobile DNA element could be influenced by the same factors that control transposition of the element.

Teras, Riho; Horak, Rita; Kivisaar, Maia

2000-01-01

309

Isolation and characterization of autolysis-defective mutants of Staphylococcus aureus created by Tn917-lacZ mutagenesis.  

PubMed

Two autolysis-defective mutants (Lyt-1 and Lyt-2) of Staphylococcus aureus have been isolated by transposon Tn917-lacZ mutagenesis. The mutants exhibited normal growth rate, cell division, cell size, and adaptive responses to environmental changes. No autolytic activities were detected in a crude autolytic enzyme preparation from the Lyt- mutants. The rate of autolysis of whole cells and cell walls in the mutants were negligible, but mutant cell wall preparations were degraded by crude enzyme preparations from the wild-type strain. Zymographic analyses of enzyme extracts from the mutants showed a single autolytic enzyme band, compared with more than 10 autolytic enzyme bands from the parent strain. Analyses of intracellular and exoprotein fractions gave results similar to those in experiments with total-cell extracts. Southern blot analysis indicated the insertion of a single copy of the transposon into the chromosome of Lyt mutants. Isogenic Lyt mutants constructed by phage phi 11 transduction showed similar phenotypes. Because both Lyt- mutants had Tn917-lacZ inserted in the appropriate orientation, it was possible to determine gene activity under various conditions by measuring beta-galactosidase activity. The gene activity was found to be induced by low pH, low temperature, and high sucrose and high sodium chloride concentrations. From these data, we propose that the mutation lies in either a master regulatory gene or a structural gene which is responsible for the synthesis or processing of a majority of the autolytic enzyme bands. PMID:8095258

Mani, N; Tobin, P; Jayaswal, R K

1993-03-01

310

A Tn3 derivative that can be used to make short in-frame insertions within genes.  

PubMed Central

A Tn3 derivative was constructed to make small in-frame insertions within genes. The transposon contains the URA3 gene, the tetA gene, a truncated lacZ, and phage P1 loxP recombination sites at either end. Insertions that have fused lacZ to an open reading frame are lac+ because they express the truncated lacZ. In the presence of the phage P1 cyclization recombinase cre, the transposon can delete the URA3, tetA, and lacZ genes between the two loxP sites. The remaining short imperfect palindrome contains the ends of Tn3 and a loxP site and does not contain a translational termination codon in the correct reading frame. We have analyzed several insertions within the yeast HO gene. Several insertions inactivate HO and prohibit initiation of mating-type switching. In contrast, an epitope inserted in the central portion encodes a functional HO endonuclease. Images

Hoekstra, M F; Burbee, D; Singer, J; Mull, E; Chiao, E; Heffron, F

1991-01-01

311

Spink County Comprehensive Plan. Part I.  

National Technical Information Service (NTIS)

The report discusses the historical background, geographical and physical factors, social and economic factors, major issues facing the County, problem areas, and opportunities that can assist the County toward achieving its goals and objectives.

1973-01-01

312

Profile of Health: Boone County, Arkansas.  

National Technical Information Service (NTIS)

The 1970 Boone County, Arkansas community education report on the availability of health facilities in the county includes a brief description of each of the 43 areas of concern (disease, disabilities, or environmental hazards) and operational facilities....

1970-01-01

313

Profile of Health Baxter County, Arkansas.  

National Technical Information Service (NTIS)

The 1970 Baxter County, Arkansas community education report on the availability of health facilities in the county includes a brief description of each of the 43 areas of concern (diseases, disabilities, or environmental hazards) and the operating facilit...

1970-01-01

314

Profile of Health: Newton County, Arkansas.  

National Technical Information Service (NTIS)

The 1970 Newton County, Arkansas community education report on the availability of health facilities in the county also includes a brief description of each of the 43 areas of concern (diseases, disabilities, or environmental hazards) and the operating fa...

1970-01-01

315

Suffolk County Comprehensive Ambulatory Health Center Model.  

National Technical Information Service (NTIS)

A model for the organization and delivery of ambulatory health services in county health centers is presented. Developed by the Suffolk County (New York) Department of Health Services, the model is structured by program function. Each service is modular i...

K. E. Gaul W. L. Markowitz

1975-01-01

316

Brevard County Near Shore Ocean Nutrification Analysis.  

National Technical Information Service (NTIS)

In response to public concerns regarding the possibility of elevated nutrients in the Brevard County surf zone, Brevard County and Canaveral Port Authority officials contracted the National Oceanic and Atmospheric Administration (NOAA). Atlantic Oceanogra...

T. P. Carsey J. R. Proni R. Ferry K. D. Goodwin J. Z. Zhang

2005-01-01

317

County Profiles. Health Service Area 7 Texas.  

National Technical Information Service (NTIS)

Information is provided by county on disease, mortality, and morbidity to accompany the health systems plan and annual implementation plan developed by the Northeast Texas Health Systems Agency, Inc. The section titled Profiles by County contains statisti...

1978-01-01

318

33. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PLAN OF STONE PIER FOR EAST END, SUTLIFF'S FERRY BRIDGE, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

319

35. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. SPECIFICATIONS FOR BRIDGE OVER THE CEDAR RIVER AT SUTLIFF'S FERRY JOHNSON COUNTY, IOWA - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

320

1. Historic American Buildings Survey Colusa County Chamber of Commerce ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Historic American Buildings Survey Colusa County Chamber of Commerce Booklet - Album of the 'County of Good Luck' Original: 1908 Re-photo: September 1940 - Hall of Records & County Jail, Colusa, Colusa County, CA

321

The Changing Responsibilities of County Governments: Data from a National Survey of County Leaders  

Microsoft Academic Search

This article reports findings from a national survey of county administrators, executives, and commission chairs from counties with an executive or an administrator that indicate counties are battling to meet rising administrative and policy demands with inadequate authority. An examination of 23 different policy areas revealed that modern counties confront some of our society's most vexing problems, most of which

Gregory Streib; William L. Waugh

1991-01-01

322

33 CFR 100.905 - Door County Triathlon; Door County, WI.  

Code of Federal Regulations, 2010 CFR

... 1 2010-07-01 2010-07-01 false Door County Triathlon; Door County, WI. 100.905 Section 100.905 Navigation...SAFETY OF LIFE ON NAVIGABLE WATERS § 100.905 Door County Triathlon; Door County, WI. (a) Regulated Area. A regulated...

2010-07-01

323

33 CFR 100.905 - Door County Triathlon; Door County, WI.  

Code of Federal Regulations, 2010 CFR

... 1 2009-07-01 2009-07-01 false Door County Triathlon; Door County, WI. 100.905 Section 100.905 Navigation...SAFETY OF LIFE ON NAVIGABLE WATERS § 100.905 Door County Triathlon; Door County, WI. (a) Regulated Area. A regulated...

2009-07-01

324

Fresno County Mock Trial Competition.  

ERIC Educational Resources Information Center

THE FOLLOWING IS THE FULL TEXT OF THIS DOCUMENT: The Fresno County Office of Education and the Fresno Unified School District hosted the Mock Trial Competition. The state competition is sponsored by the Constitutional Rights Foundation, with cosponsorship from the California State Bar Association and the California Young Lawyer's Association. This…

Fresno City Unified School District, CA.

325

Groundwater Availability in Champaign County.  

National Technical Information Service (NTIS)

Groundwater in Champaign County normally can be obtained from one of three primary water-bearing sand and gravel units within the glacial drift materials. These materials were deposited on top of the bedrock formations during three ages of glaciation. The...

E. W. Sanderson E. Zewde

1976-01-01

326

Hydrogeology of Webb County, Texas  

USGS Publications Warehouse

Introduction: Webb County, in semiarid South Texas on the U.S.-Mexico border, is a region confronted by increasing stresses on natural resources. Laredo (fig. 1), the largest city in Webb County (population 193,000 in 2000), was one of the 10 fastest-growing metropolitan areas in the country during 1990-2000 (Perry and Mackun, 2001). Commercial and industrial activities have expanded throughout the region to support the maquiladora industry (manufacturing plants in Mexico) along the border and other growth as a result of the passage of the North American Free Trade Agreement. The Rio Grande currently (2002) is the primary source of public water supply for Laredo and other cities along the border in Webb County (fig. 1). Other cities, such as Bruni and Mirando City in the southeastern part of the county, rely on ground-water supplies to meet municipal demands. Increased water demand associated with development and population growth in the region has increased the need for the City of Laredo and Webb County to evaluate alternative water sources to meet future demand. Possible options include (1) supplementing the surface-water supply with ground water, and (2) applying artificial storage and recovery (ASR) technology to recharge local aquifers. These options raise issues regarding the hydraulic capability of the aquifers to store economically substantial quantities of water, current or potential uses of the resource, and possible effects on the quality of water resulting from mixing ground water with alternative source waters. To address some of these issues, the U.S. Geological Survey (USGS), in cooperation with the City of Laredo, began a study in 1996 to assess the ground-water resources of Webb County. A hydrogeologic study was conducted to review and analyze available information on the hydrogeologic units (aquifers and confining units) in Webb County, to locate available wells in the region with water-level and water-quality information from the aquifers, and to analyze the hydraulic properties of the aquifers. The purpose of this report is to document the findings of the study. The information is organized by hydrogeologic unit and presented on this and six other sheets.

Lambert, Rebecca B.

2004-01-01

327

Tourism Potential in Lincoln County, Nevada  

Microsoft Academic Search

SUMMARY Lincoln County has a large potential for tourism growth. The quiet rural lifestyle found in the county is a complete contrast to that found in the populated areas around Las Vegas. The county has extensive resources to offer, such as: abundant nature; wildlife; country life experiences; and cultural heritage in mining, ghost towns, farming, railway and ancient Native American

Holly Gatzke

328

Community Types and Mortality in Georgia Counties  

ERIC Educational Resources Information Center

|Using an "ecological regional analysis" methodology for defining types of communities and their associated mortality rates, this study of Georgia's 159 counties finds that the suburban and town centered counties have low mortality while the city-centered type predicts low mortality for the whites. The military-centered counties do not predict.…

Young, Frank W.

2012-01-01

329

Overview energy analysis of Sarasota County  

Microsoft Academic Search

The objective of this study is to provide county decision-makers and citizens with an overview of the energy situation in Sarasota County; and identify the major energy supply and demand problems which will face Sarasota County in the near future. Simple overview models are used to help describe and understand the complex interactions of energy supply and demand. The following

Sipe

1978-01-01

330

Once Distressed, Jackson County Moves On.  

ERIC Educational Resources Information Center

In 1981, Jackson County, North Carolina, appeared on the first list of "distressed counties" in Appalachia. Since then, the rural county has made significant improvements by investing in its physical infrastructure (which also promotes tourism); fostering economic-development partnerships among governments, small businesses, and local colleges;…

Baldwin, Fred D.

1998-01-01

331

Government Study Commissions and County Home Rule  

Microsoft Academic Search

Most of the state action on county home rule occurred in the 1970s and most of the 149 charters were adopted in the 1970s. Although there is still interest in home rule, it is largely confined to a few states and specific counties. In Pennsylvania there are six county home rule charters, and five of the six were approved in

David Hamilton

2004-01-01

332

Determinants of county charter home rule  

Microsoft Academic Search

The article explores the issue of county charter home rule. Using the comparative state policy framework, the determinants of state decisions to grant counties charter home rule are identified. The data in this study suggest that growth spawns citizen pressures for more urban services and consequently more county discretionary authority through the mechanism of charter home rule. Whether states respond

Lawrence L. Martin; Ronald C. Nyhan

1994-01-01

333

76 FR 30152 - East Calloway County Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of...  

Federal Register 2010, 2011, 2012, 2013

...FRL-9310-4] East Calloway County Middle School Mercury Spill Site, Murray, Calloway County...the East Calloway County Middle School Mercury Spill Site located in Murray, Calloway...County [[Page 30153

2011-05-24

334

High-frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by broad-host-range plasmid RP1 carrying mercury transposon Tn501.  

PubMed Central

Insertion of the mercury resistance transposon Tn501 into broad-host-range plasmid RP1 greatly enhanced the ability of this plasmid to promote chromosome transfer in the photosynthetic bacterium Rhodopseudomonas sphaeroides. Compared with the wild-type RP1, which produced less than 10(-8) recombinants per donor cell, RP1::Tn501 produced between 10(-3) and 10(-7) recombinants per donor cell depending upon the marker selected. Plasmid RP1::Tn501 promoted polarized transfer of the chromosome from one or perhaps two origins on the chromosome, giving rise to two linkage groups. All of the biosynthetic and antibiotic resistance genes that have been mapped, including those involved in photosynthesis, occur on one or another of these linkage groups.

Pemberton, J M; Bowen, A R

1981-01-01

335

Isolation of high frequency of recombination donors from Tn5 chromosomal mutants of Pseudomonas putida PPN and recalibration of the genetic map.  

PubMed

A Tn5 loaded derivative of the IncP-10 plasmid R91-5 (pMO75) was used as a suicide vector to generate random chromosomal insertion mutations in Pseudomonas putida PPN. Reintroduction of pMO75 into such mutants resulted in integration of the plasmid at the site of Tn5 insertion, giving rise to two classes of high frequency of donors recombination (Hfr) donors, transferring chromosome at high frequency (greater than 10(-1) per donor cell) in opposite directions. Consequently, Tn5 induced auxotrophic mutations could be equated with or distinguished from previously mapped mutations, and closely linked markers ordered, on the basis of marker recovery using the two classes of Hfr donor. The isolation of many new transfer origins allowed more accurate time-of-entry analysis than previously possible and resulted in the reduction of the genetic map from 103 min to 88 min. PMID:2174392

Strom, A D; Hirst, R; Petering, J; Morgan, A

1990-11-01

336

Nucleotide sequence analysis of a transposon (Tn5393) carrying streptomycin resistance genes in Erwinia amylovora and other gram-negative bacteria.  

PubMed Central

A class II Tn3-type transposable element, designated Tn5393 and located on plasmid pEa34 from streptomycin-resistant strain CA11 of Erwinia amylovora, was identified by its ability to move from pEa34 to different sites in plasmids pGEM3Zf(+) and pUCD800. Nucleotide sequence analysis reveals that Tn5393 consists of 6,705 bp with 81-bp terminal inverted repeats and generates 5-bp duplications of the target DNA following insertion. Tn5393 contains open reading frames that encode a putative transposase (tnpA) and resolvase (tnpR) of 961 and 181 amino acids, respectively. The two open reading frames are separated by a putative recombination site (res) consisting of 194 bp. Two streptomycin resistance genes, strA and strB, were identified on the basis of their DNA sequence homology to streptomycin resistance genes in plasmid RSF1010. StrA is separated from tnpR by a 1.2-kb insertion element designated IS1133. The tnpA-res-tnpR region of Tn5393 was detected in Pseudomonas syringae pv. papulans Psp36 and in many other gram-negative bacteria harboring strA and strB. Except for some strains of Erwinia herbicola, these other gram-negative bacteria lacked insertion sequence IS1133. The prevalence of strA and strB could be accounted for by transposition of Tn5393 to conjugative plasmids that are then disseminated widely among gram-negative bacteria. Images

Chiou, C S; Jones, A L

1993-01-01

337

The Chromate-Inducible chrBACF Operon from the Transposable Element TnOtChr Confers Resistance to Chromium(VI) and Superoxide?  

PubMed Central

Large-scale industrial use of chromium(VI) has resulted in widespread contamination with carcinogenic chromium(VI). The abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. Here we report identification of the transposon-located (TnOtChr) chromate resistance genes from the highly tolerant strain Ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mM. The 7,189-bp-long TnOtChr of the mixed Tn21/Tn3 transposon subfamily contains a group of chrB, chrA, chrC, and chrF genes situated between divergently transcribed resolvase and transposase genes. The chrB and chrA genes, but not chrF or chrC, were essential for establishment of high resistance in chromium-sensitive O. tritici. The chr promoter was strongly induced by chromate or dichromate, but it was completely unresponsive to Cr(III), oxidants, sulfate, or other oxyanions. Plasmid reporter experiments identified ChrB as a chromate-sensing regulator of chr expression. Induction of the chr operon suppressed accumulation of cellular Cr through the activity of a chromate efflux pump encoded by chrA. Expression of chrB, chrC, or chrF in an Escherichia coli sodA sodB double mutant restored its aerobic growth in minimal medium and conferred resistance to superoxide-generating agents menadione and paraquat. Nitroblue tetrazolium staining on native gels showed that ChrC protein had superoxide dismutase activity. TnOtChr appears to represent a mobile genetic system for the distribution of the chromate-regulated resistance operon. The presence of three genes protecting against superoxide toxicity should provide an additional survival advantage to TnOtChr-containing cells in the environments with multiple redox-active contaminants.

Branco, Rita; Chung, Ana Paula; Johnston, Tatiana; Gurel, Volkan; Morais, Paula; Zhitkovich, Anatoly

2008-01-01

338

Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system.  

PubMed Central

The human alpha-3/4-fucosyltransferase III (Fuc-TIII) participates in the synthesis of Lewis determinants. The enzyme from human sources is scarce and heterogeneous. In this paper we describe the expression of a secreted form of Fuc-TIII (SFT3) in two insect cell lines, Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn), using the baculovirus expression system. The Sf9 cells secreted approx. 0.4 unit/l (1 mg/l) of the enzyme. The Tn cells secreted approx. 3-fold this amount. A large proportion of active protein was accumulated in the two cell lines (50 and 75% respectively for Sf9 and Tn cells, on the fourth day after infection) indicating a possible limitation not only of the folding machinery, but also a saturation of the secretory pathway. SFT3 was purified by cation-exchange chromatography followed by affinity chromatography. The enzyme from the Tn cell line had a lower global charge, possibly due to post-translational modifications, such as phosphorylation or sulphation. The two glycosylation sites from SFT3 were occupied. SFT3 secreted by Sf9 cells was completely deglycosylated by peptide-N-glycanase F, whereas 50% of SFT3 secreted by Tn cells was resistant to deglycosylation by this enzyme. The apparent kinetic parameters determined with the type I acceptor were k(cat)=0.4 s(-1) and K(m)=0.87 mM for the SFT3 secreted by Tn cells, and k(cat)=0.09 s(-1) and K(m)=0.76 mM for the SFT3 secreted by Sf9 cells, indicating that the enzymes had substrate affinities within the same order of magnitude as their mammalian counterpart. Furthermore, SFT3 secreted by either cell type showed a clear preference for type 1 carbohydrate acceptors, similarly to human Fuc-TIII.

Morais, V A; Serpa, J; Palma, A S; Costa, T; Maranga, L; Costa, J

2001-01-01

339

Annual Performance Evaluation of a Pair of Energy Efficient Houses (WC3 and WC4) in Oak Ridge, TN  

SciTech Connect

Beginning in 2008, two pairs of energy-saver houses were built at Wolf Creek in Oak Ridge, TN. These houses were designed to maximize energy efficiency using new ultra-high-efficiency components emerging from ORNL s Cooperative Research and Development Agreement (CRADA) partners and others. The first two houses contained 3713 square feet of conditioned area and were designated as WC1 and WC2; the second pair consisted of 2721 square feet conditioned area with crawlspace foundation and they re called WC3 and WC4. This report is focused on the annual energy performance of WC3 and WC4, and how they compare against a previously benchmarked maximum energy efficient house of a similar footprint. WC3 and WC4 are both about 55-60% more efficient than traditional new construction. Each house showcases a different envelope system: WC3 is built with advanced framing featured cellulose insulation partially mixed with phase change materials (PCM); and WC4 house has cladding composed of an exterior insulation and finish system (EIFS). The previously benchmarked house was one of three built at the Campbell Creek subdivision in Knoxville, TN. This house (CC3) was designed as a transformation of a builder house (CC1) with the most advanced energy-efficiency features, including solar electricity and hot water, which market conditions are likely to permit within the 2012 2015 period. The builder house itself was representative of a standard, IECC 2006 code-certified, all-electric house built by the builder to sell around 2005 2008.

Biswas, Kaushik [ORNL; Christian, Jeffrey E [ORNL; Gehl, Anthony C [ORNL; Jackson, Roderick K [ORNL; Boudreaux, Philip R [ORNL

2012-04-01

340

Monroe County Library System: Pathfinders  

NSDL National Science Digital Library

Named after President James Monroe, Monroe County is part of upstate New York. The industrial metropolis of Rochester sits within its boundaries, and one of its key institutions is the Monroe County Library System. Over the past several years, the System has created this treasure trove of digital collections which dissect and organize the history of the area through historic maps, architectural guides, and an online history tour of places in Rochester that played a role in the abolition movement and the Civil War. First-time visitors might want to start out by looking over the "Raising the Flag: Patriotism in Rochester, 1892-1922" photo gallery and then move on the Susan B. Anthony Letters Collection. Library staff members have also created nine fine research guides for scholars and others, and they include a guide to Rochester immigrants and a house history guide.

341

The global regulator H-NS binds to two distinct classes of sites within the Tn10 transpososome to promote transposition.  

PubMed

The histone-like nucleoid structuring protein (H-NS) is a global transcriptional regulator that influences stress response and virulence pathways in Gram-negative bacteria. H-NS also promotes Tn10 transposition by binding directly to the transpososome and inducing a conformational change in the transpososome that favours intermolecular transposition events. H-NS binds preferentially to curved DNA and can bend non-curved DNA, it self-oligomerizes and can interact with other proteins. To determine what functions of H-NS are important in promoting Tn10 transposition, we have examined the ability of two mutant forms of H-NS, P116S and 1-64, to act in Tn10 transposition. We provide evidence that the initial interaction of H-NS with the transpososome is dependent on H-NS binding to a specific structure in DNA flanking the transposon end. Additional molecules of H-NS then bind within the transposon end. This latter event appears to be directed by H-NS binding to the Tn10 transposase protein, and is important in maintaining the transpososome in a conformation that promotes intermolecular transposition. The binding of H-NS to a transposase protein is a novel function for this important regulatory molecule. PMID:17501923

Ward, Chris M; Wardle, Simon J; Singh, Randeep K; Haniford, David B

2007-05-01

342

The Nucleoid Binding Protein H-NS Acts as an Anti-Channeling Factor to Favor Intermolecular Tn10 Transposition and Dissemination  

Microsoft Academic Search

Dissemination of the bacterial transposon Tn10 is limited by target site channeling, a process wherein the transposon ends are forced to interact with and insert into a target site located within the transposon. Integration host factor (IHF) promotes this self-destructive event by binding to the transpososome and forming a DNA loop close to one or both transposon ends; this loop

Randeep K. Singh; Janine Liburd; Simon J. Wardle; David B. Haniford

2008-01-01

343

Molecular Analysis of Tn1546-Like Elements in Vancomycin-Resistant Enterococci Isolated from Patients in Europe Shows Geographic Transposon Type Clustering  

PubMed Central

Resistance mechanism relatedness was studied in 18 clinical, European vanA vancomycin-resistant enterococci. Molecular analysis revealed 10 Tn1546-like elements, suggesting two evolutionary lineages. Lineage I dominated the European mainland, and lineage II dominated the United Kingdom and Israel. Geographic clustering reflected different types of meat consumption between countries, since each lineage is associated with colonization of different animals.

Schouten, Marinus A.; Willems, Rob J. L.; Kraak, Wilma A. G.; Top, Janetta; Hoogkamp-Korstanje, Jacomina A. A.; Voss, Andreas

2001-01-01

344

Bacteroides fragilis Transfer Factor Tn5520: the Smallest Bacterial Mobilizable Transposon Containing Single Integrase and Mobilization Genes That Function in Escherichia coli  

Microsoft Academic Search

Many bacterial genera, including Bacteroides spp., harbor mobilizable transposons, a class of transfer factors that carry genes for conjugal DNA transfer and, in some cases, antibiotic resistance. Mobilizable transposons are capable of inserting into and mobilizing other, nontransferable plasmids and are implicated in the dissem- ination of antibiotic resistance. This paper presents the isolation and characterization of Tn5520, a new

GAYATRI VEDANTAM; THOMAS J. NOVICKI; DAVID W. HECHT

1999-01-01

345

Organization of the Antiseptic Resistance Gene qacA and Tn552Related  Lactamase Genes in Multidrug- Resistant Staphylococcus haemolyticus Strains of Animal and Human Origins  

Microsoft Academic Search

A part (12 kb) of a plasmid containing the -lactamase genes of Tn552, the disinfectant resistance gene qacA, and flanking DNA has been cloned from a Staphylococcus haemolyticus isolate and sequenced. This region was used to map the corresponding regions in six other multiresistant S. haemolyticus isolates of human and animal origin. The organizations of the genetic structures were almost

I.-L. Anthonisen; M. Sunde; T. M. Steinum; M. S. Sidhu; H. Sorum

2002-01-01

346

Generation of Mini-Tn10 transposon insertion mutant library of Bacillus thuringiensis for the investigation of genes required for its bacteriocin production.  

PubMed

Bacillus thuringiensis strain BUPM4 is known for its ability to produce a bacteriocin, called Bacthuricin F4 (BF4), which inhibits the growth of several Gram-positive bacteria and particularly Bacillaceae. This study aimed to use the insertional transposon mutagenesis approach for disrupting and thus identifying genes associated with BF4 synthesis. Here, the mini-Tn10 transposon was used to generate a library of B. thuringiensis mutants. Twenty thousand clones were screened for the search of mutants with affected bacteriocin synthesis. By molecular hybridization, it was demonstrated that the mini-Tn10 transposition occurred in different sites. Clone MB1, containing a mini-Tn10 single-copy insertion, lost the BF4 synthesis, but maintained its immunity to BF4. The flanking sequences surrounding the mini-Tn10 insertion were cloned and sequenced. Homology searches of the surrounding ORFs revealed a strong similarity to a phage tail component, which allowed us to postulate that BUPM4 bacteriocin could be a phage tail-like one. PMID:19431236

Kamoun, Fakher; Fguira, Ines Ben; Tounsi, Afef; Abdelkefi-Mesrati, Lobna; Sanchis, Vincent; Lereclus, Didier; Jaoua, Samir

2009-05-01

347

Drainage basins in St. Johns County, Florida  

USGS Publications Warehouse

The highest point in St. Johns County, Florida is only slightly above 65 feet above sea level and drainage divides are indistinct and readily influenced by cultural features. This report delineates some 80 drainage basins (or artificial equivalents to natural basins such as the Intracoastal Waterway) within the county and their areas in square miles. Total area of St. Johns County is about 670 square miles. (USGS)

Foose, Donald W.

1984-01-01

348

Iowa County Citizens Speak Out on Health. Results of the Iow County Health Attitudes Survey.  

National Technical Information Service (NTIS)

Results of a survey conducted by the Iowa County Health Resource Committee to determine the health attitudes of Iowa County, Wisconsin citizens are reported. A questionnaire was mailed to 340 providers and consumers; 225 citizens responded (66 percent) . ...

1973-01-01

349

Water resources of Lehigh County, Pennsylvania  

USGS Publications Warehouse

Lehigh County occupies an area of 347 square miles in southeastern Pennsylvania. The northern part of Lehigh County is underlain by the Martinsburg Formation, which consists chiefly of shale and slate. The central part of the county, where most of the population centers are located and much of the urbanization is occurring, is underlain by alternating beds of limestone and dolomite. From oldest to youngest, these carbonate rocks are the Leithsville Formation, the Allentown Formation, the Beekmantown Group, and the Jacksonburg Formation. The southern part of the county is underlain chiefly by the shales, sandstones, and conglomerates of the Brunswick Formation and by metamorphic and igneous rocks.

Wood, Charles R.; Flippo, Herbert N., Jr.; Lescinsky, Joseph B.; Barker, James L.

1972-01-01

350

32. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

32. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PROFILE OF HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY IN JOHNSON COUNTY, IOWA, 1897. (RIGHT 1/4 OF DRAWING) PLAN OF APPROACH FOR SUTLIFF'S FERRY BRIDGE OVER CEDAR RIVER, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

351

Landsliding in Allegheny County, Pennsylvania  

USGS Publications Warehouse

Man should proceed with caution if modifications such as loading, excavation, or changes of the water regime are contemplated for slopes in Allegheny County, especially those slopes described on the map as highly sensitive to disturbance by man. Features indicative of unstable slope conditions include: cracks in buildings, yard walls, and pools; doors and windows that jam; fences and other linear features bowed out of line; tilted trees and utility poles; cracks and steplike ground features; hummocky ground; and water seeps. Geologic factors related to the landsliding process include rock types, layering, fracturing, and attitude; nature of soil cover; permeability of rocks and soils; and steepness of slope.

Briggs, Reginald Peter; Pomeroy, John S.; Davies, William E.

1975-01-01

352

Transposon Tn7 Preferentially Inserts into GAAoTTC Triplet Repeats under Conditions Conducive to YoRoY Triplex Formation  

PubMed Central

Background Expansion of an unstable GAA•TTC repeat in the first intron of the FXN gene causes Friedreich ataxia by reducing frataxin expression. Structure formation by the repeat has been implicated in both frataxin repression and GAA•TTC instability. The GAA•TTC sequence is capable of adopting multiple non-B DNA structures including Y•R•Y and R•R•Y triplexes. Lower pH promotes the formation of Y•R•Y triplexes by GAA•TTC. Here we used the bacterial transposon Tn7 as an in vitro tool to probe whether GAA•TTC repeats can attract a well-characterized recombinase. Methodology/Principal Findings Tn7 showed a pH-dependent preference for insertion into uninterrupted regions of a Friedreich ataxia patient-derived repeat, inserting 48, 39 and 14 percent of the time at pH 7, pH 8 and pH 9, respectively. Moreover, Tn7 also showed orientation and region specific insertion within the repeat at pH 7 and pH 8, but not at pH 9. In contrast, transposon Tn5 showed no strong preference for or against the repeat during in vitro transposition at any pH tested. Y•R•Y triplex formation was reduced in predictable ways by transposon interruption of the GAA•TTC repeat. However, transposon interruptions in the GAA•TTC repeats did not increase the in vitro transcription efficiency of the templates. Conclusions/Significance We have demonstrated that transposon Tn7 will recognize structures that form spontaneously in GAA•TTC repeats and insert in a specific orientation within the repeat. The conditions used for in vitro transposition span the physiologically relevant range suggesting that long GAA•TTC repeats can form triplex structures in vivo, attracting enzymes involved in DNA repair, recombination and chromatin modification.

Mancuso, Miriam; Sammarco, Mimi C.; Grabczyk, Ed

2010-01-01

353

77 FR 73005 - Revisions to the California State Implementation Plan, Imperial County, Placer County, and...  

Federal Register 2010, 2011, 2012, 2013

EPA is proposing to approve revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD) and Ventura County Air Pollution Control District (PCAPCD) portions of the California State Implementation Plan (SIP). Under authority of the Clean Air Act as amended in 1990 (CAA or the Act), we are proposing to approve local rules that......

2012-12-07

354

76 FR 12280 - Revisions to the California State Implementation Plan, for Imperial County, Kern County, and...  

Federal Register 2010, 2011, 2012, 2013

EPA is taking direct final action to approve revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District (KCAPCD), and Ventura County Air Pollution Control District (VCAPCD) portions of the California State Implementation Plan (SIP). Under authority of the Clean Air Act as amended in 1990 (CAA or the Act), we are approving local rules that......

2011-03-07

355

76 FR 12306 - Revisions to the California State Implementation Plan, Imperial County, Kern County, and Ventura...  

Federal Register 2010, 2011, 2012, 2013

EPA is proposing to approve revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District (KCAPCD), and Ventura County Air Pollution Control District (VCAPCD) portions of the California State Implementation Plan (SIP). We are proposing to approve revisions to local rules that define terms used in other air pollution regulations in these areas......

2011-03-07

356

The Tn Antigen-Specific Lectin from Ground Ivy Is an Insecticidal Protein with an Unusual Physiology1  

PubMed Central

Leaves of ground ivy (Glechoma hederacea) contain a lectin (called Gleheda) that is structurally and evolutionary related to the classical legume lectins. Screening of a population of wild plants revealed that Gleheda accounts for more than one-third of the total leaf protein in some clones, whereas it cannot be detected in other clones growing in the same environment. Gleheda is predominantly expressed in the leaves where it accumulates during early leaf maturation. The lectin is not uniformly distributed over the leaves but exhibits a unique localization pattern characterized by an almost exclusive confinement to a single layer of palisade parenchyma cells. Insect feeding trials demonstrated that Gleheda is a potent insecticidal protein for larvae of the Colorado potato beetle (Leptinotarsa decemlineata). Because Gleheda is not cytotoxic, it is suggested that the insecticidal activity is linked to the carbohydrate-binding specificity of the lectin, which as could be demonstrated by agglutination assays with different types of polyagglutinable human erythrocytes is specifically directed against the Tn antigen structure (N-acetylgalactosamine O-linked to serine or threonine residues of proteins).

Wang, Weifang; Hause, Bettina; Peumans, Willy J.; Smagghe, Guy; Mackie, Anne; Fraser, Robin; Van Damme, Els J.M.

2003-01-01

357

Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif.  

PubMed

Bacterial DNA transposition is an important model system for studying DNA recombination events such as HIV-1 DNA integration and RAG-1-mediated V(D)J recombination. This communication focuses on the role of protein-phosphate contacts in manipulating DNA structure as a requirement for transposition catalysis. In particular, the participation of the nontransferred strand (NTS) 5' phosphate in Tn5 transposition strand transfer is analyzed. The 5' phosphate plays no direct catalytic role, nonetheless its presence stimulates strand transfer approximately 30-fold. X-ray crystallography indicates that transposase-DNA complexes formed with NTS 5' phosphorylated DNA have two properties that contrast with structures formed with complexes lacking the 5' phosphate or complexes generated from in-crystal hairpin cleavage. Transposase residues R210, Y319 and R322 of the (R)YREK motif coordinate the 5' phosphate rather than the subterminal NTS phosphate, and the 5' NTS end is moved away from the 3' transferred strand end. Mutation R210A impairs the 5' phosphate stimulation. It is posited that DNA phosphate coordination by R210, Y319 and R322 results in movement of the 5' NTS DNA away from the 3'-end thus allowing efficient target DNA binding. It is likely that this role for the newly identified RYR triad is utilized by other transposase-related proteins. PMID:18790806

Klenchin, Vadim A; Czyz, Agata; Goryshin, Igor Y; Gradman, Richard; Lovell, Scott; Rayment, Ivan; Reznikoff, William S

2008-09-12

358

Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif  

PubMed Central

Bacterial DNA transposition is an important model system for studying DNA recombination events such as HIV-1 DNA integration and RAG-1-mediated V(D)J recombination. This communication focuses on the role of protein–phosphate contacts in manipulating DNA structure as a requirement for transposition catalysis. In particular, the participation of the nontransferred strand (NTS) 5? phosphate in Tn5 transposition strand transfer is analyzed. The 5? phosphate plays no direct catalytic role, nonetheless its presence stimulates strand transfer ?30-fold. X-ray crystallography indicates that transposase–DNA complexes formed with NTS 5? phosphorylated DNA have two properties that contrast with structures formed with complexes lacking the 5? phosphate or complexes generated from in-crystal hairpin cleavage. Transposase residues R210, Y319 and R322 of the (R)YREK motif coordinate the 5? phosphate rather than the subterminal NTS phosphate, and the 5? NTS end is moved away from the 3? transferred strand end. Mutation R210A impairs the 5? phosphate stimulation. It is posited that DNA phosphate coordination by R210, Y319 and R322 results in movement of the 5? NTS DNA away from the 3?-end thus allowing efficient target DNA binding. It is likely that this role for the newly identified RYR triad is utilized by other transposase-related proteins.

Klenchin, Vadim A.; Czyz, Agata; Goryshin, Igor Y.; Gradman, Richard; Lovell, Scott; Rayment, Ivan; Reznikoff, William S.

2008-01-01

359

The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region.  

PubMed Central

We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed the same amounts of beta-galactosidase, while translational fusions varied 35-fold in expression efficiency. The mRNA half-lives varied 24-fold, with 9.6 min for the most highly expressed mRNA and 0.4 min for the least efficiently expressed mRNA. Toeprint experiments were performed to distinguish whether these mutations define a determinant of mRNA stability or influence translation initiation. The highly expressed mRNA was 24-fold more efficient in forming the initiation complex in vitro than the low-expression mutant. It was concluded that this sequence, albeit located upstream of the ribosome-binding sequence, is an important determinant for efficient initiation of translation. Secondary-structure calculations of the mRNAs revealed no correlation of the potential to form double strands masking the ribosome-binding sequence with expression efficiency. Images

Flache, P; Baumeister, R; Hillen, W

1992-01-01

360

Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif  

SciTech Connect

Bacterial DNA transposition is an important model system for studying DNA recombination events such as HIV-1 DNA integration and RAG-1-mediated V(D)J recombination. This communication focuses on the role of protein-phosphate contacts in manipulating DNA structure as a requirement for transposition catalysis. In particular, the participation of the nontransferred strand (NTS) 5' phosphate in Tn5 transposition strand transfer is analyzed. The 5' phosphate plays no direct catalytic role, nonetheless its presence stimulates strand transfer {approx}30-fold. X-ray crystallography indicates that transposase-DNA complexes formed with NTS 5' phosphorylated DNA have two properties that contrast with structures formed with complexes lacking the 5' phosphate or complexes generated from in-crystal hairpin cleavage. Transposase residues R210, Y319 and R322 of the (R)YREK motif coordinate the 5' phosphate rather than the subterminal NTS phosphate, and the 5' NTS end is moved away from the 3' transferred strand end. Mutation R210A impairs the 5' phosphate stimulation. It is posited that DNA phosphate coordination by R210, Y319 and R322 results in movement of the 5' NTS DNA away from the 3'-end thus allowing efficient target DNA binding. It is likely that this role for the newly identified RYR triad is utilized by other transposase-related proteins.

Klenchin, Vadim A.; Czyz, Agata; Goryshin, Igor Y.; Gradman, Richard; Lovell, Scott; Rayment, Ivan; Reznikoff, William S. (UW); (Polish)

2009-01-15

361

Transposon Mutagenesis in Bifidobacterium breve: Construction and Characterization of a Tn5 Transposon Mutant Library for Bifidobacterium breve UCC2003  

PubMed Central

Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities.

Ruiz, Lorena; Motherway, Mary O'Connell; Lanigan, Noreen; van Sinderen, Douwe

2013-01-01

362

112. 'PROPOSED CIRCULATION SYSTEM.' FROM FAIRFIELD COUNTY PLANNING ASSOCIATION, FAIRFIELD: ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

112. 'PROPOSED CIRCULATION SYSTEM.' FROM FAIRFIELD COUNTY PLANNING ASSOCIATION, FAIRFIELD: THE FIRST PLANNED COUNTY IN NEW ENGLAND. JUNE 1933. - Merritt Parkway, Beginning in Greenwich & running 38 miles to Stratford, Greenwich, Fairfield County, CT

363

113. "EXISTING MAJOR HIGHWAYS". FROM FAIRFIELD COUNTY PLANNING ASSOCIATION, FAIRFIELD: ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

113. "EXISTING MAJOR HIGHWAYS". FROM FAIRFIELD COUNTY PLANNING ASSOCIATION, FAIRFIELD: THE FIRST PLANNED COUNTY IN NEW ENGLAND. JUNE 1933 - Merritt Parkway, Beginning in Greenwich & running 38 miles to Stratford, Greenwich, Fairfield County, CT

364

111. 'PROPOSED CIRCULATION SYSTEM.' FROM FAIRFIELD COUNTY PLANNING ASSOCIATION, FAIRFIELD: ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

111. 'PROPOSED CIRCULATION SYSTEM.' FROM FAIRFIELD COUNTY PLANNING ASSOCIATION, FAIRFIELD: THE FIRST PLANNED COUNTY IN NEW ENGLAND, JUNE 1933. - Merritt Parkway, Beginning in Greenwich & running 38 miles to Stratford, Greenwich, Fairfield County, CT

365

75 FR 23222 - Kern and Tulare Counties Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013

...DEPARTMENT OF AGRICULTURE Forest Service Kern and Tulare Counties Resource Advisory Committee...SUMMARY: The first meeting of the Kern and Tulare Counties Resource Advisory Committee...funding. The purpose of the Tulare and Kern Counties Resource Advisory Committee...

2010-05-03

366

75 FR 54846 - Kern and Tulare Counties Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013

...DEPARTMENT OF AGRICULTURE Forest Service Kern and Tulare Counties Resource Advisory Committee...SUMMARY: The Kern and Tulare Counties Resource Advisory Committee...meetings will be held at the County of Kern Administrative Office, 1115 Truxtun...

2010-09-09

367

75 FR 69154 - Environmental Impact Statement: Kern County, CA  

Federal Register 2010, 2011, 2012, 2013

...Administration Environmental Impact Statement: Kern County, CA AGENCY: Federal Highway Administration...proposed 24th Street Improvement Project in Kern County, California, is being rescinded...the 24th Street Improvement Project in Kern County, California. The proposed...

2010-11-10

368

76 FR 22863 - Kern and Tulare Counties Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013

...DEPARTMENT OF AGRICULTURE Forest Service Kern and Tulare Counties Resource Advisory Committee...SUMMARY: The Kern and Tulare Counties Resource Advisory Committee...meetings will be held at the County of Kern Administrative Office, 1115 Truxtun...

2011-04-25

369

77 FR 43571 - Kern and Tulare Counties Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013

...DEPARTMENT OF AGRICULTURE Forest Service Kern and Tulare Counties Resource Advisory Committee...SUMMARY: The Kern and Tulare Counties Resource Advisory Committee...floor conference room of the County of Kern Administrative Office, 1115 Truxtun...

2012-07-25

370

75 FR 54419 - Environmental Impact Statement: Yellowstone County, MT  

Federal Register 2010, 2011, 2012, 2013

...Administration Environmental Impact Statement: Yellowstone County, MT AGENCY: Federal Highway...prepared for a proposed highway project in Yellowstone County, Montana. FOR FURTHER INFORMATION...312 in or near the city of Billings, Yellowstone County, Montana. FHWA previously...

2010-09-07

371

75 FR 54297 - Tri-County Advisory Committee Meeting  

Federal Register 2010, 2011, 2012, 2013

...DEPARTMENT OF AGRICULTURE Forest Service Tri-County Advisory Committee Meeting AGENCY...Beaverhead-Deerlodge National Forest's Tri-County Resource Advisory Committee will...FURTHER INFORMATION CONTACT: Patty Bates, Tri County Resource Advisory Committee...

2010-09-07

372

Educational and Demographic Profile: Kern County.  

ERIC Educational Resources Information Center

This profile derived from U.S. Census 2000 data presents educational and socioeconomic information for Kern County, California, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for…

California Postsecondary Education Commission, 2004

2004-01-01

373

Public Library Service for San Benito County.  

ERIC Educational Resources Information Center

|A sparsely populated, agricultural area, San Benito County (California) provides library services in conjunction with the Hollister city library and in cooperation with the San Juan Bautista city library. Financing comes from the county general fund. There are no written goals or policy statements and no professionally trained librarians. As…

McGovern, Gail

374

Commodity Flow Study - Clark County, Nevada, USA  

SciTech Connect

The United States Department of Energy has designated Clark County, Nevada as an 'Affected Unit of Local Government' due to the potential for impacts by activities associated with the Yucca Mountain High Level Nuclear Waste Repository project. Urban Transit, LLC has led a project team of transportation including experts from the University of Nevada Las Vegas Transportation Research Center to conduct a hazardous materials community flow study along Clark County's rail and truck corridors. In addition, a critical infrastructure analysis has also been carried out in order to assess the potential impacts of transportation within Clark County of high level nuclear waste and spent nuclear fuel to a proposed repository 90 miles away in an adjacent county on the critical infrastructure in Clark County. These studies were designed to obtain information relating to the transportation, identification and routing of hazardous materials through Clark County. Coordinating with the United States Department of Energy, the U.S. Department of Agriculture, the U. S. Federal Highway Administration, the Nevada Department of Transportation, and various other stakeholders, these studies and future research will examine the risk factors along the entire transportation corridor within Clark County and provide a context for understanding the additional vulnerability associated with shipping spent fuel through Clark County. (authors)

Conway, S.Ph.D. [Urban Environmental Research LLC, Las Vegas, NV (United States); Navis, I. [AICP Planning Manager, Clark County Nuclear Waste Division, Department of Comprehensive Planning, Las Vegas, NV (United States)

2008-07-01

375

COUNTIES OF CALIFORNIA - WITH FARM STATISTICS  

EPA Science Inventory

County farming statistics polygon coverage of California. The statistics were taken from the 1997 Census of Agriculture and added to a coverage extracted from TIGER county boundaries. A farm is any place from which $1,000 or more of agricultural products were sold, or normally w...

376

A Profile of Hardee County, Florida.  

ERIC Educational Resources Information Center

|In 1977 leaders of Hardee County, Florida, listed relationships and attitudes of residents, rural atmosphere, environmental conditions, and economic potential among the county's strong points, and public service and facility improvements, developing economic potential, recreational and entertainment development, and planning and zoning as its…

Beaulieu, Lionel J.; Anderson, Deborah S.

377

NEW HAZARD MAPS FOR RIVERSIDE COUNTY, CALIFORNIA  

Microsoft Academic Search

Riverside County, California is a complex area of differing geologic and hydrologic conditions representing many differing hazards. Hazard iden- tification defines the magnitude and associated probabilities of natural haz- ards that may pose threats to human interests in specific areas. Utilizing geographical information systems (GIS), the following maps of the Temecula area of Riverside County were developed: raster image, digital

W. Richard Laton; Rene Perez; Doug Bausch

378

Educational and Demographic Profile: Sacramento County.  

ERIC Educational Resources Information Center

|This profile uniquely presents a variety of educational and socioeconomic information for Sacramento County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

California Postsecondary Education Commission, 2004

2004-01-01

379

COUNTY BOUNDARIES FOR THE MAIA STUDY REGION  

EPA Science Inventory

This data set is a geographic information system (GIS) coverage of the county boundaries in the United States Environmental Protection Agency (USEPA) Mid-Atlantic Integrated Assessment (MAIA) Project region. The coverage was produced using the US Geological Survey county bound...

380

Groundwater management and protection Madison County, Alabama  

SciTech Connect

Groundwater is extremely important to Madison County as it provides nearly three quarters of the county's drinking water. In recent years, Madison County has increasingly recognized the need to protect its groundwater resource. A supply of usable groundwater is one element of a high quality environment, which can help spur economic development and provide for the needs of a growing population. Without planning protection and understanding of possible consequences, however, economic development and population pressures can cause a gradual degradation of groundwater. In April 1987, the Tennessee Valley Authority (TVA) convened a local groundwater steering group in Madison County. At the first meeting the ground agreed upon these goals: (1) to seek incorporate groundwater protection into the planning and development process for Madison County, (2) to support efforts by Madison County to obtain authority to adopt zoning ordinances and subdivision regulations, and (3) to develop a groundwater management plan for the county. This report provides essential information needed in developing a plan and is based on the following assumptions: the citizens of Madison County value the environment in which they live and wish to protect it from pollution; continued economic development is necessary for a healthy local economy; and a healthy economy can be sustained and nurtured, without degradation of the groundwater resource, through countywide planning, education, and participation.

French, J.H.; Strunk, J.W.

1990-07-01

381

Analysis of County School Districts in Arkansas.  

ERIC Educational Resources Information Center

|The 1948, Arkansas School District Reorganization Act was passed in an effort to reduce the 1589 small school districts to a smaller number. Those districts not consolidated would form county districts. As of the 1967-68 school year, 26 of these county districts remained. The purpose of this study was to provide information drawing attention to…

Budd, Karol B.; Charlton, J.L.

382

Socioeconomic Heterogeneity of Mining-Dependent Counties.  

ERIC Educational Resources Information Center

|Although the socioeconomic well-being of all U.S. mining-dependent counties was slightly above the national average in 1990, disaggregation reveals substantial effects of region and mining subsector. In particular, southern and Great Lakes coal-mining counties had significantly lower high school graduation rates and higher poverty and…

Nord, Mark; Luloff, A. E.

1993-01-01

383

Mobile Homes Study, Sullivan County, New York.  

National Technical Information Service (NTIS)

The study was initiated with the recognition that the mobile homes will be increasing in Sullivan County and that methods of controlling them are essential if the county is to maintain its essential character and support its tax base. Mobile homes impose ...

1971-01-01

384

Geothermal development issues: Recommendations to Deschutes County  

SciTech Connect

This report discusses processes and issues related to geothermal development. It is intended to inform planners and interested individuals in Deschutes County about geothermal energy, and advise County officials as to steps that can be taken in anticipation of resource development. (ACR)

Gebhard, C.

1982-07-01

385

Ada County Sheriff's Office Internet Site.  

National Technical Information Service (NTIS)

The purpose of this report is to document the development and current status of the Ada County Sheriff's office Internet site. On January 1, 1997, Boise State University and the Ada County Sheriff's Office entered into a partnership whose purpose was to a...

2002-01-01

386

County Data Book 1995: Kentucky Kids Count.  

ERIC Educational Resources Information Center

This data book presents findings of the Kids Count project on current conditions faced by Kentucky children age birth through 19. For each county, and for the state, comparisons are provided between the base years of 1980-1982 and the most recent years 1992-1994. Counties are ranked against each other and trend graphs are provided for the studied…

Kentucky Youth Advocates, Inc., Louisville.

387

County Data Book 1997: Kentucky Kids Count.  

ERIC Educational Resources Information Center

This Kids Count data book examines trends in the well-being of Kentucky's children on a statewide and county basis. An introduction summarizes some of the trends for Kentucky's children in the 1990s. The bulk of the report presents statewide and county data grouped into five categories: (1) poverty rates and programs (persons in poverty; median…

Kentucky Kids Count Consortium.

388

Land Use Plan, Saluda County, South Carolina.  

National Technical Information Service (NTIS)

The report presents a plan for future land use in Saluda County, S.C. It is based upon analyses of physical and socio-economic determinants which are expected to influence development in the county between now and 1990. The report suggests programs for im...

1973-01-01

389

A FLORISTIC STUDY OF WASHINGTON COUNTY, OKLAHOMA  

Microsoft Academic Search

The county is located principally within the Claremore Cuesta Plains geomorphic province. The Eastern Sandstone Cuesta Plains province extends into the western edge of the county at various points. These two provinces contain Pennsylvanian series sandstones and limestones that cap ridges between broad shale plains (3). The ridges have a steep face on the east and a gentle backslope with

Charles B. McDonald

1976-01-01

390

Cowley County Community College Plan of Action.  

ERIC Educational Resources Information Center

|The document presents a comprehensive plan for developing Cowley County Community College and Vocational-Technical School into a stronger community institution. It currently has over 1,400 students enrolled in a variety of general, occupational, and continuing education programs with more than half of the students coming from five counties in…

Nelson, Gwen

391

Environmental assessment: Deaf Smith County site, Texas  

SciTech Connect

In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and the eight other potentially sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines. On the basis of these findings, the DOE is nominating the Deaf Smith County site as one of the five sites suitable for characterization. 591 refs., 147 figs., 173 tabs.

Not Available

1986-05-01

392

Imperial County geothermal development annual meeting: summary  

SciTech Connect

All phases of current geothermal development in Imperial County are discussed and future plans for development are reviewed. Topics covered include: Heber status update, Heber binary project, direct geothermal use for high-fructose corn sweetener production, update on county planning activities, Brawley and Salton Sea facility status, status of Imperial County projects, status of South Brawley Prospect 1983, Niland geothermal energy program, recent and pending changes in federal procedures/organizations, plant indicators of geothermal fluid on East Mesa, state lands activities in Imperial County, environmental interests in Imperial County, offshore exploration, strategic metals in geothermal fluids rebuilding of East Mesa Power Plant, direct use geothermal potential for Calipatria industrial Park, the Audubon Society case, status report of the Cerro Prieto geothermal field, East Brawley Prospect, and precision gravity survey at Heber and Cerro Prieto geothermal fields. (MHR)

Not Available

1983-01-01

393

77 FR 61020 - Notice of Availability of Final Environmental Impact Statement for the Sigurd to Red Butte No. 2...  

Federal Register 2010, 2011, 2012, 2013

...Corps of Engineers; National Park Service; the State of Utah; Utah School and Institutional Trust Lands Administration; Millard, Sevier, Beaver, Iron, and Washington Counties, Utah; and the cities of St. George and Enterprise, Utah. In...

2012-10-05

394

76 FR 53926 - Utah; Major Disaster and Related Determinations  

Federal Register 2010, 2011, 2012, 2013

...have been designated as adversely affected by this major disaster: Beaver, Box Elder, Cache, Daggett, Duchesne, Emery, Millard, Morgan, Piute, Salt Lake, Sanpete, Sevier, Summit, Tooele, Uintah, Utah, Wasatch, and Weber Counties and the...

2011-08-30

395

76 FR 30962 - Notice of Availability of Draft Environmental Impact Statement for the Sigurd to Red Butte No. 2...  

Federal Register 2010, 2011, 2012, 2013

...USFS (Dixie and Fishlake National Forests); U.S. Army Corps of Engineers; National Park Service; State of Utah; Millard, Sevier, Beaver, Iron, and Washington counties, Utah; and the cities of St. George and Enterprise, Utah. In...

2011-05-27

396

76 FR 65509 - Environmental Impact Statements; Notice of Availability  

Federal Register 2010, 2011, 2012, 2013

...Development, and Production of Mineral and Energy Resources and Reclamation of Activities, Beaver, Garfield, Iron, Juab, Millard, Piute, Sanpete, Sevier, and Wayne Counties, UT, Comment Period Ends: 12/05/2011, Contact: Diane Freeman...

2011-10-21

397

76 FR 50807 - Utah Disaster #UT-00010  

Federal Register 2010, 2011, 2012, 2013

...determined to be adversely affected by the disaster: Primary Counties: Beaver, Box Elder, Cache, Daggett, Duchesne, Emery, Millard, Morgan, Piute, Salt Lake, Sanpete, Sevier, Summit, Tooele, Uintah, Utah, Wasatch, Weber, and the Uintah and...

2011-08-16

398

77 FR 9652 - Environmental Impacts Statements; Notice of Availability  

Federal Register 2010, 2011, 2012, 2013

...Development, and Production of Mineral and Energy Resources and Reclamation of Activities, Beaver, Garfield, Iron, Juab, Millard, Piute, Sanpete, Sevier, and Wayne Counties, UT, Comment Period Ends: 04/02/2012, Contact: Diane Freeman...

2012-02-17

399

Analysis of the Shallow Groundwater Flow System at Fire Island National Seashore, Suffolk County, New York  

USGS Publications Warehouse

Fire Island National Seashore (FIIS) occupies 42 kilometers of the barrier island for which it is named that lies off the southern shore of Suffolk County, N.Y. Freshwater in the highly permeable, sandy aquifer underlying Fire Island is bounded laterally by marine surface waters and at depth by saline groundwater. Interspersed throughout FIIS are 17 pre-existing residential communities that in summer months greatly increase in population through the arrival of summer residents and vacationers; in addition, the National Park Service (NPS) has established several facilities on the island to accommodate visitors to FIIS. The 2.2 million people estimated by the NPS to visit Fire Island annually impact groundwater quality through the release of waste-derived contaminants, such as nutrients, pathogens, and organic compounds, into the environment. Waste-contaminated groundwater can move through the aquifer and threaten the ecological health of the adjacent back-barrier estuaries to which much of the groundwater ultimately discharges. In 2004, the U.S. Geological Survey (USGS), in cooperation with the NPS, began a 3-year investigation to (1) collect groundwater levels and water-quality (nutrient) samples, (2) develop a three-dimensional model of the shallow (water-table) aquifer system and adjacent marine surface waters, and (3) calculate nitrogen loads in simulated groundwater discharges from the aquifer to back-barrier estuaries and the ocean. The hydrogeology of the shallow aquifer system was characterized from the results of exploratory drilling, geophysical surveying, water-level monitoring, and water-quality sampling. The investigation focused on four areas-the communities of Kismet and Robbins Rest, the NPS Visitor Center at Watch Hill, and the undeveloped Otis Pike Fire Island High Dune Wilderness. Thirty-five observation wells were installed within FIIS to characterize subsurface hydrogeology and establish a water-table monitoring network in the four study areas. A variable-density model of the shallow aquifer system and adjacent marine surface waters was developed to simulate groundwater flow patterns and rates. Nitrogen loads from the shallow aquifer system were calculated from representative total nitrogen (TN) concentrations and simulated groundwater discharges to back-barrier estuaries and the ocean. The model simulates groundwater directions, velocities, and discharge rates under 2005 mean annual conditions. Groundwater budgets were developed for recharge areas of similar land use that contribute freshwater to back-barrier estuaries, the ocean, and subsea-discharge zones. Total freshwater discharge from the shallow aquifer system is about 43,500 cubic meters per day (m3/d) (79.8 percent) to back-barrier estuaries and about 10,200 m3/d (18.7 percent) to the ocean; about 836 m3/d (1.5 percent) may exit the system as subsea underflow. The total contribution of fresh groundwater to shoreline discharge zones amounts to about 53,700 m3/d (98.5 percent). The median age of freshwater discharged to back-barrier estuaries and the ocean was 3.4 years, and the 95th-percentile age was 20 years. The TN concentrations and loads under 2005 mean annual conditions for areas that contribute fresh groundwater to back-barrier estuaries and the ocean were calculated for the principal land uses on Fire Island. The overall TN load from the shallow aquifer system to shoreline discharge zones is about 16,200 kilograms per year (kg/yr) (82.2 percent) to back-barrier estuaries and about 3,500 kg/yr (17.8 percent) to the ocean. The overall TN load to marine surface waters amounts to about 19,700 kg/yr-roughly 6 percent of the annual TN load from shallow groundwater entering the South Shore Estuary Reserve (SSER) from the Suffolk County mainland, which is about 345,000 kg/yr. In contrast to the TN load from shallow groundwater for the SSER watershed, which annually yields about 353 kilograms per square kilometer (kg/km2), the overall TN loa

Schubert, Christopher E.

2010-01-01

400

Shoreham Railroad Bridge, Former Addison County Railroad (later, Rutland Railroad, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Shoreham Railroad Bridge, Former Addison County Railroad (later, Rutland Railroad, Addison Branch), spanning Lemon Fair River above Richville Pond, west of East Shoreham Road, Shoreham, Addison County, VT

401

7. Photocopy of photograph (from Broome County Historical Society) showing ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photocopy of photograph (from Broome County Historical Society) showing SWIMMERS, PHOTOGRAPH TAKEN FACING NORTHEAST - Charles F. Johnson Pool, Charles F. Johnson Park, Johnson City, Broome County, NY

402

Costilla County Biodiesel Pilot Project  

SciTech Connect

The Costilla County Biodiesel Pilot Project has demonstrated the compatibility of biodiesel technology and economics on a local scale. The project has been committed to making homegrown biodiesel a viable form of community economic development. The project has benefited by reducing risks by building the facility gradually and avoiding large initial outlays of money for facilities and technologies. A primary advantage of this type of community-scale biodiesel production is that it allows for a relatively independent, local solution to fuel production. Successfully using locally sourced feedstocks and putting the fuel into local use emphasizes the feasibility of different business models under the biodiesel tent and that there is more than just a one size fits all template for successful biodiesel production.

Doon, Ben; Quintana, Dan

2011-08-25

403

Nolley v. County of Erie.  

PubMed

The U.S. District Court, Western District of New York, held that the Erie County Holding Center (ECHC) had violated an HIV-positive inmate's privacy rights under the U.S. Constitution and the New York Public Health Law by placing red stickers on the inmate's paperwork and belongings, and by segregating her from the general inmate population. The court reasoned that, pursuant to the federal Centers for Disease Control's recommendation that corrections staff take HIV precautions with all inmates, the ECHC's treatment constituted an unjustifiable isolation based solely on her HIV status. The court also held that the ECHC further violated the inmate's constitutional rights by denying her access to the law library and to religious services. Although the court found the inmate's incarceration conditions "deplorable," the conditions did not constitute "cruel and unusual punishment." The court also found no federal Rehabilitation Act violation because the ECHC did not receive federal financial assistance. PMID:11648225

1991-10-31

404

Hydrologic conditions: Dade County, Florida  

USGS Publications Warehouse

Thin layers of dense limestone of low permeability that occur near the top of the Biscayne aquifer in the vicinity of the north end of Levee 30 in Dade County, Florida are of hydrologic importance because they retard the downward infiltration of ponded water in Conservation Area No. 3. This retarding effect frequently results in high head differentials across the levee. Tests made in a small area adjacent to Levee 30 indicate that the coefficient of transmissibility of the aquifer is 3,600,000 gpd (gallons per day) per foot, and the coefficient of vertical permeability of the dense limestones is 13 gpd per square foot. If ground-water flow beneath the levee is laminar, the total inflow to the Levee 30 Canal from Conservation Area No. 3 will be about 350 mgd (million gallons per day), or 540 cfs (cubic feet per second), per mile length of levee when the head difference across the levee is 10 feet.

Kohout, Francis Anthony; Klein, Howard; Sherwood, C. B.; Leach, Stanley D.

1964-01-01

405

Nucleotide sequence analysis of the termini and chromosomal locus involved in site-specific integration of the streptococcal conjugative transposon Tn5252.  

PubMed Central

The 47-kb, broad-host-range, streptococcal conjugative transposon Tn5252 is capable of site-specific integration into the pneumococcal chromosome. We present the nucleotide sequence of the terminal regions of the transposon and its target site in the pneumococcal genome. No inverted repeats were found at the termini of the transposon. A 72-bp region of the target was present on either side following the insertion of Tn5252 and appeared to serve as a signal for its integration and excision. The data suggest that the left copy of the 72-bp segment was a part of the conjugative element, the crossover point of integration was nonrandom within this region, and the mechanism of insertion could resemble that of the site-specific temperate phages. Images

Vijayakumar, M N; Ayalew, S

1993-01-01

406

Cyclic changes in the affinity of protein-DNA interactions drive the progression and regulate the outcome of the Tn10 transposition reaction  

PubMed Central

The Tn10 transpososome is a DNA processing machine in which two transposon ends, a transposase dimer and the host protein integration host factor (IHF), are united in an asymmetrical complex. The transitions that occur during one transposition cycle are not limited to chemical cleavage events at the transposon ends, but also involve a reorganization of the protein and DNA components. Here, we demonstrate multiple pathways for Tn10 transposition. We show that one series of events is favored over all others and involves cyclic changes in the affinity of IHF for its binding site. During transpososome assembly, IHF is bound with high affinity. However, the affinity for IHF drops dramatically after cleavage of the first transposon end, leading to IHF ejection and unfolding of the complex. The ejection of IHF promotes cleavage of the second end, which is followed by restoration of the high affinity state which in turn regulates target interactions.

Liu, Danxu; Crellin, Paul; Chalmers, Ronald

2005-01-01

407

Hfq restructures RNA-IN and RNA-OUT and facilitates antisense pairing in the Tn10/IS10 system  

PubMed Central

Hfq functions in post-transcriptional gene regulation in a wide range of bacteria, usually by promoting base-pairing of mRNAs and trans-encoded sRNAs that share partial sequence complementarity. It is less clear if Hfq is required for pairing of cis-encoded RNAs (i.e., antisense RNAs) with their target mRNAs. In the current work, we have characterized the interactions between Escherichia coli Hfq and the components of the Tn10/IS10 antisense system, RNA-IN and RNA-OUT. We show that Hfq interacts with RNA-OUT through its proximal RNA-binding surface, as is typical for Hfq and trans-encoded sRNAs. In contrast, RNA-IN binds both proximal and distal RNA-binding surfaces in Hfq with a higher affinity for the latter, as is typical for mRNA interactions in canonical sRNA-mRNA pairs. Importantly, an amino acid substitution in Hfq that interferes with RNA binding to the proximal site negatively impacts RNA-IN:OUT pairing in vitro and suppresses the ability of Hfq to negatively regulate IS10 transposition in vivo. We also show that Hfq binding to RNA-IN and RNA-OUT alters secondary structure elements in both of these RNAs and speculate that this could be important in how Hfq facilitates RNA-IN:OUT pairing. Based on the results presented here, we suggest that Hfq could be involved in regulating RNA pairing in other antisense systems, including systems encoded by other transposable elements.

Ross, Joseph A.; Ellis, Michael J.; Hossain, Shahan; Haniford, David B.

2013-01-01

408

Impacts of cool cities on air quality: A preliminary modeling assessment for Nashville TN, Dallas TX and Atlanta GA  

SciTech Connect

Previous atmospheric modeling efforts that concentrated on the Los Angeles Basin suggested beneficial and significant air quality impacts from cool cities strategies. This paper discusses an extension of similar modeling efforts to three regions, Atlanta GA, Dallas - Ft. Worth TX, and Nashville TN, that experience smog and air quality problems. According to the older ozone air quality standard (120 ppb), these regions were classified as serious, moderate, and marginal, respectively, but may be out of compliance with respect to the newer, 80-ppb/8-hours standard. Results from this exploratory modeling work suggest a range of possible impacts on meteorological and air quality conditions. For example, peak ozone concentrations during each region's respective episode could be decreased by 1-6 ppb (conservative and optimistic scenarios, respectively) in Nashville, 5-15 ppb in Dallas - Fort Worth, and 5-12 ppb in Atlanta following implementation of cool cities. The reductions are generally smaller than those obtained from simulating the Los Angeles Basin but are still significant. In all regions, the simulations suggest, the net, domain-wide effects of cool cities are reductions in ozone mass and improvements in air quality. In Atlanta, Nashville, and Dallas, urban areas benefiting from reduced smog reach up to 8460, 7350, and 12870 km{sup 2} in area, respectively. Results presented in this paper should be taken as exploratory and preliminary. These will most likely change during a more comprehensive modeling study to be started soon with the support of the US Environmental Protection Agency. The main purpose of the present project was to obtain the initial data (emission inventories) for these regions, simulate meteorological conditions, and perform preliminary sensitivity analysis. In the future, additional regions will be simulated to assess the potential of cool cities in improving urban air quality.

Taha, Haider

1998-06-15

409

Identification and Characterization of Non-Cellulose-Producing Mutants of Gluconacetobacter hansenii Generated by Tn5 Transposon Mutagenesis.  

PubMed

The acs operon of Gluconacetobacter is thought to encode AcsA, AcsB, AcsC, and AcsD proteins that constitute the cellulose synthase complex, required for the synthesis and secretion of crystalline cellulose microfibrils. A few other genes have been shown to be involved in this process, but their precise role is unclear. We report here the use of Tn5 transposon insertion mutagenesis to identify and characterize six non-cellulose-producing (Cel(-)) mutants of Gluconacetobacter hansenii ATCC 23769. The genes disrupted were acsA, acsC, ccpAx (encoding cellulose-complementing protein [the subscript "Ax" indicates genes from organisms formerly classified as Acetobacter xylinum]), dgc1 (encoding guanylate dicyclase), and crp-fnr (encoding a cyclic AMP receptor protein/fumarate nitrate reductase transcriptional regulator). Protein blot analysis revealed that (i) AcsB and AcsC were absent in the acsA mutant, (ii) the levels of AcsB and AcsC were significantly reduced in the ccpAx mutant, and (iii) the level of AcsD was not affected in any of the Cel(-) mutants. Promoter analysis showed that the acs operon does not include acsD, unlike the organization of the acs operon of several strains of closely related Gluconacetobacter xylinus. Complementation experiments confirmed that the gene disrupted in each Cel(-) mutant was responsible for the phenotype. Quantitative real-time PCR and protein blotting results suggest that the transcription of bglAx (encoding ?-glucosidase and located immediately downstream from acsD) was strongly dependent on Crp/Fnr. A bglAx knockout mutant, generated via homologous recombination, produced only ?16% of the wild-type cellulose level. Since the crp-fnr mutant did not produce any cellulose, Crp/Fnr may regulate the expression of other gene(s) involved in cellulose biosynthesis. PMID:24013627

Deng, Ying; Nagachar, Nivedita; Xiao, Chaowen; Tien, Ming; Kao, Teh-Hui

2013-09-06

410

B lymphoblastoid cell lines with normal and defective O-glycosylation established from an individual with blood group Tn.  

PubMed

Individuals with the Tn blood group contain terminal serine/threonine-linked N-acetylgalactosamine residues in their blood cells. This is due to lack of UDP-D-galactose: D-N-acetyl galactosamine beta-D-galactosyl transferase from part of their red cells and probably from their leukocytes. We have established B lymphoblastoid cell lines from such an individual by in vitro infection of his lymphocytes with Epstein-Barr virus. The original line contained a mixture of cells reactive and nonreactive with Helix pomatia lectin (Hp). These cells were subcloned after staining with fluorescent Hp by a fluorescence-activated cell sorter (FACS) into homogeneous, phenotypically stable lines of Hp-positive (Hp+) and Hp-negative (Hp-) cells. The molecular differences between the membrane glycoproteins were characterized by carbohydrate-specific surface labeling techniques, Hp affinity chromatography, polyacrylamide slab gel electrophoresis and glycopeptide/oligosaccharide analysis. The major O-glycosidic membrane glycoprotein (GP105) was retained on Hp-Sepharose columns only from Hp+ cells, whereas the common leukocyte antigen (GP160-200) was partially retained on Hp columns from both lines. These proteins were isolated by immune precipitation with monoclonal antibodies and characterized. The results show that the GP105 glycoprotein from Hp+ cells contains terminal N-acetylgalactosamine residues but also more complex oligosaccharides. The common leukocyte antigen showed different electrophoretic mobilities in Hp+ and Hp- cells. UDP-galactose D-N-acetyl galactosamine beta-galactosyl transferase was almost absent in the Hp+ cells. These cell lines are useful for studies on the functional role and regulation of the biosynthesis of O-glycosidic carbohydrates. PMID:3006838

Gahmberg, C G; Peltokorpi, L; Andersson, L C

1986-04-01

411

Fe2+-Tetracycline-Mediated Cleavage of the Tn10 Tetracycline Efflux Protein TetA Reveals a Substrate Binding Site near Glutamine 225 in Transmembrane Helix 7  

Microsoft Academic Search

TetA specified by Tn10 is a class B member of a group of related bacterial transport proteins of 12 transmembrane alpha helices that mediate resistance to the antibiotic tetracycline. A tetracycline-divalent metal cation complex is expelled from the cell in exchange for a entering proton. The site(s) where tetracycline binds to this export pump is not known. We found that,

Laura M. McMurry; Mila L. Aldema-Ramos; Stuart B. Levy

2002-01-01

412

Characterization of Tn 1547, a composite transposon flanked by the IS 16 and IS 256-like elements, that confers vancomycin resistance in Enterococcus faecalis BM4281  

Microsoft Academic Search

A 64-kb genetic element harboring a vanB vancomycin-resistance (VmR) gene cluster was shown to translocate from the chromosome of Enterococcus faecalis BM4281 into the hemolysin (Hly) plasmid, pIP964. Sequence analysis of the resulting junction fragments indicated that the VmR genes were carried by a composite transposon, Tn1547, bounded by two distantly related insertion sequences (IS), designated IS256-like and IS16, in

Richard Quintiliani; Patrice Courvalin

1996-01-01

413

Isolation and Characterization of Mini-Tn5Km2 Insertion Mutants of Brucella abortus Deficient in Internalization and Intracellular Growth in HeLa Cells  

Microsoft Academic Search

Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in profes- sional and nonprofessional phagocytes and cause abortion in domestic animals and undulant fever in humans. The mechanism and factors of virulence are not fully understood. To identify genes related to internalization and multiplication in host cells, Brucella abortus was mutagenized by mini-Tn5Km2 transposon that

Suk Kim; Masahisa Watarai; Yuki Kondo; Janchivdorj Erdenebaatar; Sou-ichi Makino; Toshikazu Shirahata

2003-01-01

414

Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage 119 using the gentamicin resistance gene (aacC1) of Tn1696 as reporter  

Microsoft Academic Search

A promoter-probe shuttle plasmid (pGL7011) containing the promoterless aminoglycoside-O-acetyltransferase I gene (aacC7) of Tn1696 was used to isolate DNA fragments from Streptomyces ghanaensis phage 119 that possessed promoter activity in Streptomyces liwidans TK23. Analysis of gentamicin (Gm) resistance levels in Escherichia coli and in 5. liwidans TK23, and of aacC7 mRNA levels in 5. liwidans, identified a fragment (F14) that

Gabriele Labes; Mervyn Bibb; W. Wohlleben

1997-01-01

415

The isolation and characterisation of a plaque-forming derivative of bacteriophage Mu carrying a fragment of Tn 3 conferring ampicillin resistance  

Microsoft Academic Search

We have isolated a plaque-forming derivative of phage Mu which carries a determinant for ApR. The biological properties of this MuAp phage are similar to those of normal Mu. Its genome contains a 1.1 kb substitution where Mu DNA from the right end of the G region has been replaced by a similar length of DNA from the transposon Tn3.

David Leach; Neville Symonds

1979-01-01

416

Characterization of an Azospirillum brasilense Tn5 mutant with enhanced N2 ¢xation: the e¡ect of ORF280 on nifH expression  

Microsoft Academic Search

Disruption of an open reading frame (ORF) of 840 bp (280 amino acids; ORF280) in an Azospirillum brasilense Tn5 mutant resulted in a pleiotrophic phenotype. Besides an enhanced N2-fixing capacity and altered expression pattern of a nifH-gusA fusion, growth on the charged polar amino acids glutamate and arginine was severely affected. ORF280, similar to previously identified ORFs present in Bradyrhizobium

Luis Fernando Revers; Luciane Maria Pereira Passaglia; Kathleen Marchal; Jeverson Frazzon; Carlos Galindo Blaha; Jos Vanderleyden; Irene Silveira Schrank

2000-01-01

417

The Chromate-Inducible chrBACF Operon from the Transposable Element TnOtChr Confers Resistance to Chromium(VI) and Superoxide  

Microsoft Academic Search

Large-scale industrial use of chromium(VI) has resulted in widespread contamination with carcinogenic chromium(VI). The abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. Here we report identification of the transposon-located (TnOtChr) chromate resistance genes from the highly tolerant strain Ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mM. The 7,189-bp-long

Rita Branco; Ana Paula Chung; Tatiana Johnston; Volkan Gurel; Paula Morais; Anatoly Zhitkovich

2008-01-01

418

Effect of the polymer concentration on the ON/OFF states of a TN-LCD: polyvinyl alcohol vs. soy lecithin  

NASA Astrophysics Data System (ADS)

In this work we study the response of a Twisted Nematic Liquid Crystal Display (TN-LCD) by varying both the concentration and the polymer used for the microgroove. We compare the performance of two polymers: polyvinyl alcohol and soy lecithin. In particular, the light transmission for the ON/OFF states is evaluated. The polyvinyl alcohol is a polymer widely used in LCDs while lecithin soy is a natural polymer.

de Coss Martinez, Romeo; Gonzalez Murguia, Jose Luis

2011-03-01

419

Trimeric Tn Antigen on Syndecan 1 Produced by ppGalNAc-T13 Enhances Cancer Metastasis via a Complex Formation with Integrin ?5?1 and Matrix Metalloproteinase 9.  

PubMed

We demonstrated previously that ppGalNAc-T13 (T13), identified as an up-regulated gene with increased metastasis in a DNA microarray, generated trimeric Tn (tTn) antigen (GalNAc?1-Ser/Thr)3 on Syndecan 1 in highly metastatic sublines of Lewis lung cancer. However, it is not known how tTn antigen regulates cancer metastasis. Here, we analyzed the roles of tTn antigen in cancer properties. tTn antigen on Syndecan 1 increased cell adhesion to fibronectin in an integrin-dependent manner. Furthermore, cell adhesion to fibronectin induced phosphorylation of focal adhesion kinase and paxillin in T13-transfectant cells. In the search of Syndecan 1-interacting molecules, it was demonstrated that tTn antigen-carrying Syndecan 1 interacted with integrin ?5?1 and matrix metalloproteinase 9 and that these molecules shifted to a glycolipid-enriched microdomain/rafts along with increased metastatic potential in T13-transfectant cells. We also identified a tTn substitution site on Syndecan 1, demonstrating that tTn on Syndecan 1 is essential for the interaction with integrin ?5?1 as well as for the reaction with mAb MLS128. These data suggest that high expression of the ppGalNAc-T13 gene generates tTn antigen on Syndecan 1 under reduced expression of GM1, leading to enhanced invasion and metastasis via the formation of a molecular complex consisting of integrin ?5?1, Syndecan 1, and MMP-9 in the glycolipid-enriched microdomain/rafts. PMID:23814067

Matsumoto, Yasuyuki; Zhang, Qing; Akita, Kaoru; Nakada, Hiroshi; Hamamura, Kazunori; Tsuchida, Akiko; Okajima, Tetsuya; Furukawa, Keiko; Urano, Takeshi; Furukawa, Koichi

2013-06-27

420

The nucleoid binding protein H-NS acts as an anti-channeling factor to favor intermolecular Tn10 transposition and dissemination.  

PubMed

Dissemination of the bacterial transposon Tn10 is limited by target site channeling, a process wherein the transposon ends are forced to interact with and insert into a target site located within the transposon. Integration host factor (IHF) promotes this self-destructive event by binding to the transpososome and forming a DNA loop close to one or both transposon ends; this loop imposes geometric and topological constraints that are responsible for channeling. We demonstrate that a second 'host' protein, histone-like nucleoid structuring protein (H-NS), acts as an anti-channeling factor to limit self-destructive intramolecular transposition events in vitro. Evidence that H-NS competes with IHF for binding to the Tn10 transpososome to block channeling and that this event is relatively insensitive to the level of DNA supercoiling present in the Tn10-containing substrate plasmid are presented. This latter observation is atypical for H-NS, as H-NS binding to other DNA sequences, such as promoters, is generally affected by subtle changes in DNA structure. PMID:18191147

Singh, Randeep K; Liburd, Janine; Wardle, Simon J; Haniford, David B

2007-12-23

421

Drainage basins in Duval County, Florida  

USGS Publications Warehouse

The drainage basins and subbasins in Duval County, Florida, are delineated on this atlas map. The county 's 840 square-mile area is drained by three major river systems; the St. Johns, 668 square miles; Nassau, 113 square miles; and St. Marys, 59 square miles. The remainder of the county is drained by a network of small streams that flow into either the Intracoastal Waterway or directly into the Atlantic Ocean. The sub-basins range in size from less than one square mile to more than 50 square miles. (USGS)

Stone, Roy B.; Largen, Joseph B.

1983-01-01

422

1918 Influenza: A Winnebago County, Wisconsin Perspective  

PubMed Central

The population of Winnebago County in 1918 was approximately 62,000 residents. It consisted of towns supporting diverse manufacturers surrounded by farming country. For this study, records were revisited, and 1918 to 1920 influenza survivors were interviewed. A pharmacological investigation encompassing the various influenza treatments used in Wisconsin from 1918 to 1920 was documented. In 1918, over 180 individuals perished from influenza, and over 2000 cases were reported in Winnebago County, Wisconsin. Influenza returned in 1920, which some researchers refer to as the “fourth wave,” claiming nearly 50 lives in Winnebago County, Wisconsin. This study also documents the 1920 influenza wave.

Shors, Teri; McFadden, Susan H.

2009-01-01

423

34. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

34. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PLAN OF RIVER PIERS FOR HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

424

31. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PROFILE OF HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY IN JOHNSON COUNTY, IOWA, 1897. (LEFT 3/4 OF DRAWING) - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

425

Middlesex County Academy Roborocks 2004 Journal  

NSDL National Science Digital Library

Attached is the Middlesex County Academy journal for the Roborocks 2004 competition. The document details the team's progress in designing, implementing and troubleshooting the robot, course and program. Target Audience: General Public

Paterno, Enzo

2010-01-15

426

Surface-water availability, Tuscaloosa County, Alabama  

USGS Publications Warehouse

The average annual runoff, about 1,270 mgd (million gallons per day), originating in Tuscaloosa County is equivalent to 20 inches or 0.95 mgd per square mile. The Black Warrior and Sipsey Rivers, the largest streams in the county, have average flows of 5,230 mgd and 580 mgd, respectively, where they leave the county, and median annual 7-day low flows in excess of 150 mgd and 35 mgd, respectively. North River, Big Sandy Creek, and Hurricane Creek have average flows in excess of 100 mgd and median annual 7-day low flows in excess of 2 mgd. Surface water generally contains less than 100 mg/l (milligrams per liter) dissolved solids, less than 10 mg/l chloride, and is soft to moderately hard. Streams having the higher hardness and the higher dissolved-solids content are in eastern Tuscaloosa County.

Knight, Alfred L.; Davis, Marvin E.

1975-01-01

427

Camden County Land Use Update, 1986.  

National Technical Information Service (NTIS)

During the 1980 planning process, the county adopted a set of general goals concerning Resource Protection, Resource Production, and Economic and Community Development. The information contained in the 1980 Land Use Plan provided the starting point for th...

1988-01-01

428

Economic Potential Study: Avery County, North Carolina.  

National Technical Information Service (NTIS)

The report presents an analysis of population and economic potential in Avery County. Projection of total population is projected to the year 2000. The economy section includes details of education, income and the influence of major employers. The third s...

1970-01-01

429

PENNSYLVANIA GEOGRAPHIC INFORMATION SYSTEM LIBRARY: COUNTY BOUNDARIES  

EPA Science Inventory

Pennsylvania Geographic Information System (GIS) Library provides a data set of complete digitized county boundaries of the Commonwealth Of Pennsylvania at a scale of 1:24,000 from USGS topographic maps. The data format is ARC/INFO Export....

430

Environmental assessment, Deaf Smith County site, Texas  

SciTech Connect

The Nuclear Waste Policy Act of 1982 (42 USC sections 10101-10226) requires the environmental assessment of a proposed site to include a statement of the basis for nominating a site as suitable for characterization. Volume 2 provides a detailed statement evaluating the site suitability of the Deaf Smith County Site under DOE siting guidelines, as well as a comparison of the Deaf Smith County Site to the other sites under consideration. The evaluation of the Deaf Smith County Site is based on the impacts associated with the reference repository design, but the evaluation will not change if based on the Mission Plan repository concept. The second part of this document compares the Deaf Smith County Site to Davis Canyon, Hanford, Richton Dome and Yucca Mountain. This comparison is required under DOE guidelines and is not intended to directly support subsequent recommendation of three sites for characterization as candidate sites. 259 refs., 29 figs., 66 refs. (MHB)

Not Available

1986-05-01

431

American Indians of Pacific Region by County.  

National Technical Information Service (NTIS)

The report presents previously unpublished data on the American Indian population from the 1970 Census for counties in the Pacific region with 400 or more American Indians. Detailed tables contain data on characteristics such as age, sex, marital status, ...

J. R. Corn

1980-01-01

432

Stormwater Management Plan for Volusia County, Florida.  

National Technical Information Service (NTIS)

The report is concerned with the management of storm water in Volusia County, Florida. The report examines existing storm water conditions, identifies major drainage basins, sub-basins, and drainage outfalls. It also identifies needs for more detailed inv...

1974-01-01

433

Materials Inventory of Sherman County, Kansas.  

National Technical Information Service (NTIS)

The construction material resources of Sherman County are restricted to the Ogallala Formation and the more recent alluvial deposits found in the major stream valleys. The Ogallala Formation is composed chiefly of silt, sand, and gravel with some localize...

G. Hargadine D. Johnson D. Mahan

1964-01-01

434

Planning Work Program: Cumberland County, North Carolina.  

National Technical Information Service (NTIS)

The report identifies several major problems facing the Cumberland County Joint Planning Board; identifies major studies undertaken during the past ten years; and identifies those studies which should be undertaken during the next five years. Specific att...

1970-01-01

435

Environmental assessment: Deaf Smith County site, Texas  

SciTech Connect

In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of the nine potentially acceptable sites for mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines.

Not Available

1986-05-01

436

Zoning Ordinance: Wilkes County, North Carolina.  

National Technical Information Service (NTIS)

The ordinance was designed to provide zoning regulations that would be applicable to the entire Wilkes County area, exclusive of Wilkesboro and North Wilkesboro and their one-mile planning and zoning districts, and that land within the incorporated limits...

1969-01-01

437

Archeological Testing in Atoka County, Southeastern Oklahoma.  

National Technical Information Service (NTIS)

Proposed Impoundment 20 of the Lower Clear Boggy Creek Watershed Project is a single purpose floodwater retarding structure on Birch Creek in Atoka County, Oklahoma. Three archeological sites in the direct impact zone of the maximum flood pool were tested...

D. T. Hughes

1977-01-01

438

North Carolina: Caldwell County Manager, Bobby White  

Center for Drug Evaluation (CDER)

... Under current Federal law, if a county government establishes a drug ... In response to the deficiencies described in the Minnesota pharmacy board ... More results from www.fda.gov/drugs/drugsafety

439

Sope Creek Drainage Area, Cobb County, Georgia.  

National Technical Information Service (NTIS)

The amendment provides additional information about the proposed interceptor sewer system's environmental impact on the Sope Creek watershed in Cobb County, and elaborates on the steps that have been taken to preserve the historical and scenic aspects of ...

1973-01-01

440

Sope Creek Drainage Area, Cobb County, Georgia.  

National Technical Information Service (NTIS)

The Sope Creek Interceptor Sewer System is a part of Cobb County's overall program for providing regionalized wastewater collection and treatment. The primary long-term beneficial impact will be the elimination of a source of contamination upstream from A...

1973-01-01

441

Tourism Market Analysis: Cheyenne-Laramie County.  

National Technical Information Service (NTIS)

A tourism market analysis was accomplished to provide information on the characteristics of this industry in Cheyenne-Laramie County. The analysis explores opportunities for strengthening this sector of the economy. The study draws on interviews with prof...

1986-01-01

442

Agricultural Market Analysis: Cheyenne-Laramie County.  

National Technical Information Service (NTIS)

The agricultural industry is the oldest basic, or export industry in Laramie County. It has historically provided substantial income and employment opportunities for farmers, ranchers and local businesses such as retail establishments, repair and other se...

E. A. Fischer

1986-01-01

443

Western Baldwin County, AL Grid Interconnection Project  

SciTech Connect

The Objective of this Project was to provide an additional supply of electricity to the affected portions of Baldwin County, AL through the purchase, installation, and operation of certain substation equipment.

Thomas DeBell

2011-09-30

444

Primary Care Development Guide - Pierce County.  

National Technical Information Service (NTIS)

A development guide for the provision of primary health care in Pierce County, Washington is discussed. Primary care is defined as care which takes place in a practitioner's private office; mobile van; home; public, private, or free clinic; hospital outpa...

1974-01-01

445

Proposed Zoning Resolution for Pierce County.  

National Technical Information Service (NTIS)

The document constitutes a resolution by the county of Pierce, Georgia, to regulate land use distribution and placement as well as the specifications of standards for development. The Resolution also provides for the administration and enforcement of the ...

1973-01-01

446

Pierce County Nursing Home Development Guide.  

National Technical Information Service (NTIS)

In 1976 the Pierce County (Wash.) Health Council initiated a project with the objectives of (1) assessing the status of the area's long-term health care resources and (2) formulating recommendations concerning the development of these resources. This docu...

1978-01-01

447

Cobb Neck Master Plan, Charles County, Maryland.  

National Technical Information Service (NTIS)

The purpose of this Plan is to evaluate the development potentials and constraints affecting Cobb Neck, a peninsula in southern Charles County. This study evaluates the existing and proposed development patterns and their effect on environmental resources...

1988-01-01

448

Health Systems Plan for Riverside County 1976.  

National Technical Information Service (NTIS)

A health systems plan (HSP), representing the basis from which health services and facilities planning decisions will be made, is presented for Riverside County, California by the Comprehensive Health Planning Association of Imperial, Riverside, and San D...

1976-01-01

449

The Bridge of Mandolin County  

NASA Astrophysics Data System (ADS)

The Bridge of Mandolin County is a case designed to teach the general chemistry principles of molar mass, ions and aqueous reactions, solubility rules, and inorganic nomenclature. Through the instructor-facilitated class discussion, students consider the options before the Mandolin Town Council regarding deicing the newly constructed bridge connecting Mandolin with a large nearby city. The students must decipher contradictory claims made on behalf of sodium chloride, the traditional deicer, and calcium magnesium acetate, a new environmentally friendly deicer, to arrive at the most cost-effective and environmentally appropriate deicing product. As they work through the analysis they raise questions that can be addressed in a laboratory setting. Four optional role-playing experiments are included, which can be used by the students to gather information helpful to resolution of the case. The case is intended to be used over two class periods, with a laboratory period in between, though suggestions for other models are provided. Laboratory procedures include an EDTA titration for Ca2+ and Mg2+, a gravimetric analysis, a qualitative examination of ions and solubility, an introduction to freezing point depression and measurement, and an experimental design activity. This case can also successfully be used without alteration in non-majors chemistry or environmental chemistry courses, or upper-level analytical or environmental chemistry courses.

Lantz, Juliette M.; Feindt, Jenny E.; Lewellyn, Eric P. B.; Walczak, Mary M.

1999-12-01

450

Geothermal development plan: Maricopa County  

SciTech Connect

The Maricopa County Geothermal Development Plan evaluated the market potential for utilizing geothermal energy. The study identified six potential geothermal resource areas with temperatures less than 100{sup 0}C (212{sup 0}F) and in addition, four suspected intermediate temperature areas (90{sup 0} to 150{sup 0}C, 194{sup 0} to 300{sup 0}F). Geothermal resources are found to occur in and near the Phoenix metropolitan area where average population growth rates of two to three percent per year are expected over the next 40 years. Rapid growth in the manufacturing, trade and service sectors of the regional economy provides opportunities for the direct utilization of geothermal energy. A regional energy use analysis is included containing energy use and price projections. Water supplies are found to be adequate to support this growth, though agricultural water use is expected to diminish. The study also contains a detailed section matching geothermal resources to potential users. Two comparative analyses providing economic details for space heating projects are incorporated.

White, D.H.; Goldstone, L.A.

1982-08-01

451

The Water Cycle in Volusia County  

USGS Publications Warehouse

Earth's water is always in motion. The water cycle, also known as the hydrologic cycle, describes the continuous movement of water on, above, and below the Earth's surface. This fact sheet provides information about how much water moves into and out of Volusia County, and where it is stored. It also illustrates the seasonal variation in water quantity and movement using data from some of the hydrologic data collection sites in or near Volusia County, Florida.

German, Edward R.

2009-01-01

452

Edwards Aquifer Evaluation: Kinney County, Texas  

NASA Astrophysics Data System (ADS)

The Edwards Aquifer is one of the most studied and most prolific aquifers in the United States. The aquifer is a heavily fractured and faulted carbonate aquifer with transmissivities in excess of 100 ft2/s. The City of San Antonio relies upon the Edwards Aquifer as its sole source for water. Much work has been done on quantifying recharge to the aquifer and discharge from wells and acquiring aquifer characteristics from pumping tests, specific capacity tests, and geophysical logs. Although the aquifer has been well studied in Bexar County, much less is known about the Edwards Aquifer in Kinney County. This is partly due to the lower population within the county (approximately 3,500 people) relative to the eastern counties (Uvalde, Medina, Bexar, Comal, and Hays) and the great distance of Kinney County from high profile discharge areas such as the City of San Antonio and Comal and San Marcos Springs. Three key products resulted from this study: (1) exploratory well drilling and the largest aquifer test in the county that were conducted to evaluate the well yields within a 10,000 acre study area in which a drawdown of 2.5 ft approximately 1.2 miles away was observed while pumping at approximately 4,600 gpm; (2) a recharge estimate for the Edwards Aquifer within Kinney County of approximately 71,382 ac-ft/yr; and (3) locating the Brackettville Groundwater Divide from an evaluation of ground water flow direction and hydrograph analysis. These results help evaluate the complex hydraulics occurring within Kinney County and aid in development of ground water modeling that will be used in managing the Edwards Aquifer.

Khorzad, Kaveh

2003-10-01

453

Carbonizing properties of Butler County, PA, coals  

Microsoft Academic Search

Carbonizing properties of the principal coal beds of Butler County, PA, are described in this report. Eight samples, representing the Upper Freeport, Lower Freeport, Upper Kittanning, Middle Kittanning, Lower Kittanning, Clarion, and Brookville beds, were carbonized at 900°C by the Bureau of Mines-American Gas Association (BM-AGA) method. Butler County coals rank as high-volatile A bituminous and are generally characterized by

G. W. Birge; D. E. Wolfson

1962-01-01

454

Coffee and Cigarette Consumption and Perceived Effects in Recovering Alcoholics Participating in Alcoholics Anonymous in Nashville, TN  

PubMed Central

Background Alcoholics Anonymous (AA) members represent an important and relatively understudied population for improving our understanding of alcohol dependence recovery since over one million Americans participate in the program. Further insight into coffee and cigarette use by these individuals is necessary given AA members’ apparent widespread consumption and the recognized health consequences and psychopharmacological actions of these substances. Methods Volunteers were sought from all open-AA meetings in Nashville, TN during the summer of 2007 to complete a questionnaire (n=289, completion rate=94.1%) including timeline followback for coffee, cigarette, and alcohol consumption; the Alcoholics Anonymous Affiliation Scale; coffee consumption and effects questions; the Fagerstrom Test for Nicotine Dependence (FTND); and the Smoking Effects Questionnaire. Results Mean (±SD) age of onset of alcohol consumption was 15.4±4.2 years and mean lifetime alcohol consumption was 1026.0±772.8 kg ethanol. Median declared alcohol abstinence was 2.1 years (range: 0 days–41.1 years) and median lifetime AA attendance was 1000.0 meetings (range: 4–44209 meetings); average AA affiliation score was 7.6±1.5. Most (88.5%) individuals consumed coffee and approximately 33% of coffee consumers drank more than four cups per day (M=3.9±3.9). The most common self-reported reasons for coffee consumption and coffee-associated behavioral changes were related to stimulatory effects. More than half (56.9%) of individuals in AA smoked cigarettes. Of those who smoked, 78.7% consumed at least half a pack of cigarettes per day (M=21.8±12.3). Smokers’ FTND scores were 5.8±2.4; over 60% of smokers were highly or very highly dependent. Reduced negative affect was the most important subjective effect of smoking. Conclusions A greater proportion of AA participants drink coffee and smoke cigarettes in larger per capita amounts than observed in general US populations. The effects of these products as described by AA participants suggest significant stimulation and negative affect reduction. Fundamental knowledge of the quantitative and qualitative aspects of coffee and cigarette consumption among AA members will enable future research to discern their impact on alcohol abstinence and recovery.

Reich, Michael S.; Dietrich, Mary S.; Finlayson, A.J. Reid; Fischer, Edward F.; Martin, Peter R.

2008-01-01

455

69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C.L. Wood, the county engineer. Updated through the mid-1930s to show new federal aid-state roads. Compares modern system with older county system. Original scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

456

Comparison of Tn5397 from Clostridium difficile ,T n916 from Enterococcus faecalis and the CW459tet(M) element from Clostridium perfringens shows that they have similar conjugation regions but different insertion and excision modules  

Microsoft Academic Search

Comparative analysis of the conjugative transposons Tn5397 from Clostridium difficile and Tn916 from Enterococcus faecalis, and the CW459tet(M) element from Clostridium perfringens, has revealed that these tetracycline-resistance elements are closely related. All three elements contain the tet(M) resistance gene and have sequence similarity throughout their central region. However, they have very different integration\\/excision modules. Instead of the int and xis

Adam P. Roberts; Priscilla A. Johanesen; Dena Lyras; Peter Mullany; Julian I. Rood

2001-01-01

457

Immunization of Rhesus monkeys with a SialylTn–mAb17-1A conjugate vaccine co-formulated with QS-21 induces a temporary systemic cytokine release and NK cytotoxicity against tumor cells  

Microsoft Academic Search

Tumor-associated antigens resulting from aberrant glycosylation, such as the SialylTn carbohydrate antigen, are frequently\\u000a over-expressed on cancer cells and provide potential targets for cancer vaccination. Immunization of Rhesus monkeys with SialylTn\\u000a coupled to a highly immunogenic carrier molecule and formulated on aluminum hydroxide induced a strong immune response against\\u000a the carrier protein but only a moderate IgM immune response against

Ralf Kircheis; Petra Siegl; Susanne Grunt; Nicole Halanek; Hans Loibner; Geert C. Mudde; Andreas Nechansky

2007-01-01

458

The nifH, nifM and nifN genes of Azotobacter vinelandii : Characterisation by Tn 5 mutagenesis and isolation from pLAFR1 gene banks  

Microsoft Academic Search

Tn5 was introduced into Azotobacter vinelandii on a suicide vector, pGS9. Three Nif- mutants were found to carry Tn5 in nifH (MV6), in nifN (MV22), and in or near nifM (MV21), from the results of hybridisation experiments. For MV21 and MV22 this was also shown by complementation with the nif genes of Klebsiella pneumoniae on pRD1. MV6 failed to synthesis

Christina Kennedy; Rawia Gamal; Richard Humphrey; Juan Ramos; Kevin Brigle; Dennis Dean

1986-01-01

459

Beginnings of geothermal impact on county population and leadership, Imperial County, California  

SciTech Connect

A major geothermal energy development scenario is about to begin in Imperial County. Initial energy-socioeconomic interactions in the areas of population and county leadership structure are discussed. These include immigration of energy company workers, a sewage plant dispute, conflict over union affiliation of geothermal laborers, and a transmission corridor routing dispute.

Pick, J.B.; Butler, E.W.

1980-09-01

460

Reformed county government structure and service delivery performance: An integrated study of Florida counties  

Microsoft Academic Search

The most fundamental and challenging function of government is the effective and efficient delivery of services to local taxpayers and businesses. Counties, once known as the “dark continent” of American government, have recently become a major player in the provision of services. Population growth and suburbanization have increased service demands while the counties' role as service provider to incorporated residents

Alejandro Rodriguez

1999-01-01

461

76 FR 4254 - Irish Potatoes Grown in Certain Designated Counties in Idaho, and Malheur County, Oregon...  

Federal Register 2010, 2011, 2012, 2013

...AMS-FV-10-0109; FV11-945-1] Irish Potatoes Grown in Certain Designated Counties...conducted among eligible producers of Irish potatoes in certain designated counties in Idaho...order regulating the handling of Irish potatoes grown in the production area....

2011-01-25

462

Ground-Water Data in Orange County and Adjacent Counties, Texas, 1985- 90.  

National Technical Information Service (NTIS)

The lower unit of the Chicot aquifer is a major source of freshwater for Orange County Texas. In 1989 the average rate of ground-water withdrawal from the lower unit of the Chicot aquifer in Orange County for municipal and industrial use was 13.8 million ...

M. C. Kasmarek

1999-01-01

463

Public Health Services in Sixty-Seven Rural Counties: Are They Meeting the County's Medical Needs?  

ERIC Educational Resources Information Center

|Forty-four counties in Florida and 23 in Oregon with 1966 populations of less than 50,000 were selected for a study of the adequacy of public health services in rural areas. The counties were ranked on the following need variables: number of children under 5 years, persons over 65 years, Negroes, and families with incomes under $3,000. The seven…

Turk, William L.