Science.gov

Sample records for sevier county tn

  1. 75 FR 6257 - Watts Bar Reservoir Land Management Plan, Loudon, Meigs, Rhea, and Roane Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ... Watts Bar Reservoir Land Management Plan, Loudon, Meigs, Rhea, and Roane Counties, TN AGENCY: Tennessee... procedures for implementing the National Environmental Policy Act (NEPA). TVA has updated its 1988 land management plan (1988 Plan) for 16,036 acres of TVA public land on Watts Bar Reservoir in Tennessee....

  2. Testing the Extensional Detachment Paradigm: A Borehole Observatory in the Sevier Desert Basin

    E-print Network

    Christie-Blick, Nicholas

    Testing the Extensional Detachment Paradigm: A Borehole Observatory in the Sevier Desert Basin Mountain Resort in the adjacent Wasatch Range), with a one-day field trip to the Sevier Desert basin geology of the Sevier Desert detachment (Canyon Range). Interest in the Sevier Desert detachment (Fig. 3

  3. Particle Flow Visualizer Matt Moody and Matt Sevier

    E-print Network

    van den Berg, Jur

    Particle Flow Visualizer Matt Moody and Matt Sevier Advisor: Dr. Rob Stoll Project Description an economical and mobile wind tunnel that can be used in classrooms. Primary and secondary educators will use

  4. Superfund record of decision amendment (EPA Region 4): Wrigley Charcoal Superfund Site, Hickman County, Wrigley, TN, February 2, 1995

    SciTech Connect

    1995-03-01

    This decision document presents the selected Interim Remedial Action (IRA) for the Wrigley Charcoal Site, in Wrigley, Hickman County, Tennessee. The U.S. EPA has modified a wide variety of items that require immediate response action for the first step of cleanup activities to be taken at the Wrigley Charcoal Site. The major goal of these cleanup activities is to address the most serious threats at the Wrigley Charcoal Site by removing contaminated media from the Primary Site flood plain, remediating wastes at the Storage Basin, and through limited access restrictions at the Primary Site and the Storage Basin. The cleanup activities as presented in this IRA Record of Decision (ROD) Amendment will achieve significant risk reduction and will prepare the Site for future remedial activities.

  5. Relations between hinterland and foreland shortening: Sevier orogeny, central North American Cordillera

    E-print Network

    Taylor, Wanda J.; Bartley, John M.; Martin, Mark W.; Geissman, John W.; Walker, J. Douglas; Armstrong, Phillip A.; Fryxell, Joan E.

    2000-12-01

    Jurassic to early Tertiary Sevier belt) and the more internal zones to the west (central Nevada thrust belt) provide data critical to understanding the development of internal and external parts of orogens. The Garden Valley thrust system, part...

  6. 77 FR 60373 - Monroe Mountain Aspen Ecosystems Restoration Project Fishlake National Forest; Sevier and Piute...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-03

    ... Forest Service Monroe Mountain Aspen Ecosystems Restoration Project Fishlake National Forest; Sevier and... alternatives, within the Monroe Mountain Aspen Ecosystems Restoration Project area. The purpose of the Monroe Mountain Aspen Ecosystems Restoration Project is to implement land management activities that...

  7. Anatexis and metamorphism in tectonically thickened continental crust exemplified by the Sevier hinterland, western North America

    NASA Technical Reports Server (NTRS)

    Patino Douce, Alberto E.; Humphreys, Eugene D.; Johnston, A. Dana

    1990-01-01

    This paper presents a thermal and petrologic model of anatexis and metamorphism in regions of crustal thickening exemplified by the Sevier hinterland in western North America, and uses the model to examine the geological and physical processes leading to crustally derived magmatism. The results of numerical experiments show that anatexis was an inevitable end-product of Barrovian metamorphism in the thickened crust of the late Mesozoic Sevier orogenic belt and that the advection of heat across the lithosphere, in the form of mantle-derived mafic magmas, was not required for melting of metasedimentary rocks. It is suggested that, in the Sevier belt, as in other intracontinental orogenic belts, anatexis occurred in the midcrust and not at the base of the crust.

  8. Miocene unroofing of the Canyon Range during extension along the Sevier Desert Detachment, west central Utah

    E-print Network

    Stockli, Daniel F.; Linn, Jonathan K.; Walker, J. Douglas; Dumitru, Trevor A.

    2001-06-01

    fault, the Sevier Desert Detachment (SDD) fault [McDonald, 1976; Wernicke, 1981; Wernicke and Burchfiel, 1982; Allmendinger et al., 1983; Von Tish et al., 1985; Mitchell and McDonald, 1987; Planke and Smith, 1991]. The magnitude of displacement... along the proposed SSD ranges from Tish et al., 1985; Coogan and DeCelles, 1996; Mitra and Sussman, 1997]. The hypothesized SDD has become one of the most famous and classic examples for low...

  9. Inventory of wetlands and agricultural land cover in the upper Sevier River Basin, Utah

    NASA Technical Reports Server (NTRS)

    Jaynes, R. A.; Clark, L. D., Jr.; Landgraf, K. F. (principal investigator)

    1981-01-01

    The use of color infrared aerial photography in the mapping of agricultural land use and wetlands in the Sevier River Basin of south central utah is described. The efficiency and cost effectiveness of utilizing LANDSAT multispectral scanner digital data to augment photographic interpretations are discussed. Transparent overlays for 27 quadrangles showing delineations of wetlands and agricultural land cover were produced. A table summarizing the acreage represented by each class on each quadrangle overlay is provided.

  10. Influence of preexisting tectonic trends on geometries of Sevier orogenic belt and its foreland in Utah

    SciTech Connect

    Picha, F.; Gibson, R.I.

    1985-05-01

    The tectonic style of the late Mesozoic Sevier orogenic belt in Utah was greatly affected by preexisting structural trends that date from the late Precambrian rifting and fragmentation of the North American continent. The late Precambrian cratonic margin (Cordilleran hinge line) was marked by a system of prominent faults including the north-south-trending ancestral Wasatch and ancient Ephraim faults and the southwest-northeast-trending Leamington, Scipio, Cove Fort, and Paragonah faults. During the Paleozoic and Mesozoic, renewed activity on these faults affected the geometries of the late Paleozoic Paradox and Oquirrh basins, the boundaries of the Jurassic Arapien Formation, and the sedimentary pattern of the Cretaceous foreland basin. Many of these fault zones were reactivated as tectonic ramps (e.g., the ancient Ephraim fault) and tear faults (e.g., the Leamington fault) during the compressional Sevier tectonism. The Fillmore arch and some other structural highs situated along the edge of the late Precambrian craton caused ramping of the inner Keystone-Pavant-Canyon thrust sheets and telescoping of the frontal thrust sheets. Post-thrust uplift of basement highs led to tectonic denudation and to the development of low-angle, extensional faults, such as the Sevier detachment. Northeast-trending lineaments, such as the Cove Fort and Paragonah lineaments, were reactivated as right-lateral strike-slip faults. They also affected the extent of the Marysvale volcanic field.

  11. 75 FR 20774 - Establishment of Class E Airspace; Mountain City, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-21

    ... FR 63976), Docket No. FAA-2009-0061; Airspace Docket No. 09-ASO-10. The FAA uses the direct final... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Mountain City, TN AGENCY... December 7, 2009 that establishes Class E airspace at Johnson County Airport, Mountain City, TN....

  12. Preliminary Feasibility Assessment of Geologic Carbon Sequestration Potential for TVA's John Sevier and Kingston Power Plants

    SciTech Connect

    Smith, Ellen D; Saulsbury, Bo

    2008-03-01

    This is a preliminary assessment of the potential for geologic carbon sequestration for the Tennessee Valley Authority's (TVA) John Sevier and Kingston power plants. The purpose of this assessment is to make a 'first cut' determination of whether there is sufficient potential for geologic carbon sequestration within 200 miles of the plants for TVA and Oak Ridge National Laboratory (ORNL) to proceed with a joint proposal for a larger project with a strong carbon management element. This assessment does not consider alternative technologies for carbon capture, but assumes the existence of a segregated CO{sub 2} stream suitable for sequestration.

  13. Late Cretaceous extension in the hinterland of the Sevier thrust belt, northwestern Utah and southern Idaho

    SciTech Connect

    Wells, M.L.; Allmendinger, R.W. ); Dallmeyer, R.D. )

    1990-10-01

    Cover rocks of the Raft River metamorphic core complex, located in the Sevier belt hinterland, preserve a structural and metamorphic history that predates the middle Tertiary extension of the region. In the eastern Raft River Mountains, Cambrian( )-Permian rocks form two allochthons that occur in the hanging wall of the mid-Miocene Raft River detachment fault. Dramatically attenuated, metamorphosed Cambrian( )-Pennsylvanian strata of the lower allochthon yield Late Cretaceous muscovite {sup 40}Ar/{sup 39}Ar plateau ages (ca. 82-90 Ma) that are interpreted to date cooling during final stages of layer parallel extension. Devonian-Permian rocks exposed in the Black Pine Mountains underwent east-west extension ({approximately} 160%) associated with syntectonic growth of white mica along cleavage. {sup 40}Ar/{sup 39}Ar whole-rock spectra of associated slates also indicate a Late Cretaceous metamorphism. Together, field and {sup 40}Ar/{sup 39}Ar results suggest that Late Cretaceous extension occurred in the Sevier belt hinterland at the same time as shortening in the eastern foreland and at depth in the hinterland. Sufficient topography must have been present to drive upper-crustal extension in the eastern hinterland.

  14. Cretaceous crustal structure and metamorphism in the hinterland of the Sevier thrust belt, western US Cordillera

    SciTech Connect

    Miller, E.L.; Gans, P.B. )

    1989-01-01

    Combined structural and geochronologic studies to the west of the Sevier belt at lat 39{degree}-40{degree}N indicate that peak metamorphism of the upper 15 km of crust occurred during the Late Cretaceous (70-90 Ma), overlapping in time with the intrusion of muscovite-bearing granites and pegmatites. Metamorphism was accompanied by penetrative deformation (top-to-the-east layer-parallel shear); both were localized at the currently exposed structural levels, resulting in impressive vertical and lateral strain gradients and T{sub max} gradients of > 50 C/km. Inception of thrust faulting in the Sevier belt may have predated peak metamorphism and intrusion of granites in the hinterland by 10-15 m.y., but it continued during and ended simultaneously with the end of metamorphism at about 70 Ma. The rise of muscovite-bearing granites and hot fluids, generated by metamorphism and crustal anatexis at greater depth, provides and explanation for the observed localized metamorphism and deformation at relatively high structural levels.

  15. 15. Gatlinburg spur with little Pigeon River, looking toward Pigeon ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Gatlinburg spur with little Pigeon River, looking toward Pigeon Forge. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

  16. 1. Roaring Fork Motor Nature Trail, entrance sign. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  17. 7. Roaring Fork Motor Nature Trail, rocks along edge of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  18. 3. Roaring Fork Motor Nature Trail, view between second and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  19. 12. Roaring Fork Motor Nature Trail, place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  20. 5. Roaring Fork Motor Nature Trail, vista at stop three. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  1. 8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  2. 10. Reagan's Tub Mill and Roadway looking NE. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Reagan's Tub Mill and Roadway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  3. 2. Roaring Fork Motor Nature Trail, road view before first ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  4. 4. Overgrown overlook number 1 looking W. Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Overgrown overlook number 1 looking W. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  5. Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  6. 11. Roaring Fork Motor Nature Trail, boulders along road after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  7. 6. Roaring Fork Motor Nature Trail, road view after stop ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  8. 9. Roaring Fork Motor Nature Trail, Reagan House. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  9. 10. Area of landslide on eastern section of Foothills Parkway ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Area of landslide on eastern section of Foothills Parkway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

  10. 2. Deep Creek Road, old bridge at campground entrance. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Deep Creek Road, old bridge at campground entrance. - Great Smoky Mountains National Park Roads & Bridges, Deep Creek Road, Between Park Boundary near Bryson City & Deep Creek Campground, Gatlinburg, Sevier County, TN

  11. 1. Deep Creek Road, picnic pavilion Great Smoky Mountains ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Deep Creek Road, picnic pavilion - Great Smoky Mountains National Park Roads & Bridges, Deep Creek Road, Between Park Boundary near Bryson City & Deep Creek Campground, Gatlinburg, Sevier County, TN

  12. 1. View of Cades Cove Valley from first overlook on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Cades Cove Valley from first overlook on Rich Mountain Road looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  13. 24. View of barn, fields and Cades Cove loop road ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. View of barn, fields and Cades Cove loop road looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  14. 13. Cades Cove Road, with barbed wire fence and mountain ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Cades Cove Road, with barbed wire fence and mountain view. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  15. 21. View down Hyatt Lane in Cades Cove looking NNE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View down Hyatt Lane in Cades Cove looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  16. 17. Methodist Church in Cades Cove looking NW. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Methodist Church in Cades Cove looking NW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  17. 10. Concrete bridge on road to Tremont looking S. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Concrete bridge on road to Tremont looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  18. 16. First Baptist Church in Cades Cove looking SSW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. First Baptist Church in Cades Cove looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  19. 6. 1930's revetment wall next to 1886 constructed revetment wall ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. 1930's revetment wall next to 1886 constructed revetment wall on Laurel Creek Road looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  20. 28. Ford near the end of Cades Cove Loop Road ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Ford near the end of Cades Cove Loop Road looking NNW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  1. 27. Abandoned treelined road in Cades Cove looking N. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. Abandoned tree-lined road in Cades Cove looking N. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  2. 2. Switchback and General Road view looking S. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Switchback and General Road view looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  3. 14. Cades Cove Road, mountain view with single tree. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Cades Cove Road, mountain view with single tree. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  4. 1. View of Laurel Creek Road, revetment wall and cliff ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Laurel Creek Road, revetment wall and cliff looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  5. 8. Double arch culvert on Laurel Creek Road looking WSW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Double arch culvert on Laurel Creek Road looking WSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  6. 23. Henry Whitehead place in Cades Cove looking ESE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Henry Whitehead place in Cades Cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  7. 12. Cades Cove Road, view toward entrance with ruts in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Cades Cove Road, view toward entrance with ruts in road. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  8. 25. Dan Lawson place in Cades Cove looking S. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. Dan Lawson place in Cades Cove looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  9. 4. Ford near the northern end of road looking NNE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Ford near the northern end of road looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  10. 5. Laurel Creek Road, revetment wall and creek. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Laurel Creek Road, revetment wall and creek. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  11. 15. Cades Cove Road, mountain view with horses. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Cades Cove Road, mountain view with horses. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  12. 9. Double arch culvert on Laurel Creek Road looking ENE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Double arch culvert on Laurel Creek Road looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  13. 22. Cades Cove Valley from Hyatt Lane looking E. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Cades Cove Valley from Hyatt Lane looking E. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  14. 26. View of Cades Cove Loop Road with fields and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. View of Cades Cove Loop Road with fields and mountains looking NE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  15. 2. View of west tunnel portal and stream on Laurel ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View of west tunnel portal and stream on Laurel Creek Road looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  16. 18. View of fields, mountains and mist in Cades Cove ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of fields, mountains and mist in Cades Cove looking SW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  17. 7. Elevation of single arch stone bridge on Laurel Creek ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Elevation of single arch stone bridge on Laurel Creek Road looking N. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  18. 19. Cantilevered barn in Cades Cove looking SSW. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Cantilevered barn in Cades Cove looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  19. 2. Big Creek Road, worm fence and road at trailhead. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Big Creek Road, worm fence and road at trailhead. - Great Smoky Mountains National Park Roads & Bridges, Big Creek Road, Between State Route 284 & Big Creek Campground, Gatlinburg, Sevier County, TN

  20. 18. Bridge over state route 321 on unopened section of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. Bridge over state route 321 on unopened section of Foothills Parkway in wear cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

  1. 9. Bridge over State Route 321 on unopened section of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Bridge over State Route 321 on unopened section of Foothills Parkway in wear cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

  2. 6. LITTLE GREENBRIER SCHOOL CEMETERY AND PARKING AREA LOOKING S, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. LITTLE GREENBRIER SCHOOL CEMETERY AND PARKING AREA LOOKING S, OFF SPUR FROM METCALF BOTTOMS TO WEAR COVE. - Great Smoky Mountains National Park Roads & Bridges, Little River Road, Between Sugarlands Visitor Center & Townsend Wye, Gatlinburg, Sevier County, TN

  3. Stratigraphy and structure of the Sevier thrust belt and proximal foreland-basin system in central Utah: A transect from the Sevier Desert to the Wasatch Plateau

    USGS Publications Warehouse

    Lawton, T.F.; Sprinkel, D.A.; Decelles, P.G.; Mitra, G.; Sussman, A.J.; Weiss, M.P.

    1997-01-01

    The Sevier orogenic belt in central Utah comprises four north-northwest trending thrust plates and two structural culminations that record crustal shortening and uplift in late Mesozoic and early Tertiary time. Synorogenic clastic rocks, mostly conglomerate and sandstone, exposed within the thrust belt were deposited in wedge-top and foredeep depozones within the proximal part of the foreland-basin system. The geologic relations preserved between thrust structures and synorogenic deposits demonstrate a foreland-breaking sequence of thrust deformation that was modified by minor out-of-sequence thrust displacement. Structural culminations in the interior part of the thrust belt deformed and uplifted some of the thrust sheets following their emplacement. Strata in the foreland basin indicate that the thrust sheets of central Utah were emplaced between latest Jurassic and Eocene time. The oldest strata of the foredeep depozone (Cedar Mountain Formation) are Neocomian and were derived from the hanging wall of the Canyon Range thrust. The foredeep depozone subsided most rapidly during Albian through Santonian or early Campanian time and accumulated about 2.5 km of conglomeratic strata (Indianola Group). The overlying North Horn Formation accumulated in a wedge-top basin from the Campanian to the Eocene and records propagation of the Gunnison thrust beneath the former foredeep. The Canyon Range Conglomerate of the Canyon Mountains, equivalent to the Indianola Group and the North Horn Formation, was deposited exclusively in a wedge-top setting on the Canyon Range and Pavant thrust sheets. This field trip, a three day, west-to-east traverse of the Sevier orogenic belt in central Utah, visits localities where timing of thrust structures is demonstrated by geometry of cross-cutting relations, growth strata associated with faults and folds, or deformation of foredeep deposits. Stops in the Canyon Mountains emphasize geometry of late structural culminations and relationships of the Canyon Range thrust to growth strata deposited in the wedge-top depozone. Stops in the San Pitch Mountains illustrate deposits of the foredeep depozone and younger, superjacent wedge-top depozone. Stops in the Sanpete Valley and western part of the Wasatch Plateau examine the evolution of the foreland-basin system from foredeep to wedge-top during growth of a triangle zone near the front of the Gunnison thrust.

  4. Upper Oligocene evaporites in basin fill of Sevier Desert region, western Utah ( USA).

    USGS Publications Warehouse

    Lindsey, D.A.; Glanzman, R.K.; Naeser, C.W.; Nicholas, D.J.

    1981-01-01

    The basin fill beneath the Sevier Desert of western Utah contains evaporites that were deposited in a broad closed basin. All of the basin fill penetrated by the Gulf Oil 1 Gronning contains abundant volcanic detritus and its alteration products of Cenozoic age. Fission-track dating of tuffaceous sandstone yields ages of 26-28 m.y. Fossil pollen from mudstone in the evaporite-bearing strata includes forms no older than late Oligocene, in good agreement with the fission-track ages. Thus the age of evaporites is late Oligocene and younger. Anhydrite is present throughout approximately 900m of volcaniclastic rocks in the lower part of the section cut by the Gulf hole. Fossil pollen are indicative of an arid to semiarid flora.-from Authors

  5. Role of crustal thickening and extensional collapse in the tectonic evolution of the Sevier-Laramide orogeny, western United States

    SciTech Connect

    Livaccari, R.F. )

    1991-11-01

    The effects of crustal thickening and extensional collapse of continental crust, combined with variations in plate-convergence forces, provide a coherent explanation for evolution of the Cretaceous-early Tertiary Sevier-Laramide orogeny. Crustal thickening of the Sevier-Laramide hinterland region was triggered by the combination of compressive plate-convergence forces and subduction-induced conductive heating of the crust. Eastward progradation of the locus of Sevier-Laramide deformation through time reflects the progressive widening of the region of crustal thickening. The region of maximum crustal thickening migrated outward until about 80 to 75 Ma, when it encountered excessively strong lithosphere of the Colorado Plateau. The locus of deformation was then transmitted laterally across both a hinterland that had attained maximum crustal thickness and the rigid Colorado Plateau, into the Laramide Rocky Mountain foreland. An episode of vigorous extensional collapse of orogenically thickened crust affected the southern Cordillera between about 75 and 35 Ma. Early Tertiary waning of plate-convergence rates and continued subduction-induced conductive heating of thickened crust allowed extensional collapse along the southern Cordillera region to drive the Colorado Plateau block northward. The result was the late Laramide phase of Rocky Mountain foreland deformation in the central and southern Laramide Rocky Mountain region continued through Eocene time, rather than ceasing at 56 Ma, as it did in the northern Cordillera.

  6. 3. BIG HOUSE (left) AND CORN CRIB (right) IN THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. BIG HOUSE (left) AND CORN CRIB (right) IN THE BACKGROUND. See also individual HABS documentation: Walker Family Farm, Big House (HABS No. TN-121 A), and Walker Family Farm, Corn Crib (HABS No. TN-121 C). - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

  7. Upper Oligocene evaporites in basin fill of Sevier Desert region, western Utah

    SciTech Connect

    Lindsey, D.A.; Glanzman, R.K.; Naeser, C.W.; Nichols, D.J.

    1981-02-01

    The basin fill beneath the Sevier Desert of western Utah contains evaporites of late Oligocene age that were deposited in a broad closed basin. All of the basin fill penetrated by the Gulf Oil 1 Gronning (2458 m TD), one of only three deep holes in the basin, contains abundant volcanic detritus and its alteration products of Cenozoic age, when volcanism was intense in western Utah. Fission-track dating of tuffaceous sandstone, interbedded with evaporite minerals and representative of sandstone in the lower formations penetrated, yields ages of 26 to 28 m.y.; concordance of ages indicates no thermal resetting. Fossil pollen from mudstone in the evaporite-bearing strata includes forms no older than late Oligocene, in good agreement with the fission-track ages. Thus the age of the evaporites, and of all the basin fill penetrated by drilling, is late Oligocene and younger; previous work had assigned them ages ranging from Triassic to Eocene. Evaporite-bearing rocks were deposited during late Oligocene time in a broad closed basin under arid or semiarid conditions much like those of the Great Basin today. Anhydrite is present throughout approximately 900 m of volcaniclastic rocks in the lower part of the section cut by the Gulf hole, and more than 1500 m of anhydrite and halite is present in possibly equivalent strata in the nearby Argonaut dry hole. Fossil pollen from anhydrite-bearing rocks in the Gulf hole are indicative of an arid to semiarid flora, including plants similar to Mormon tea and possibly saltbush or buffalo berry. Evaporite minerals formed in the basin during dry periods; tuff was erupted from nearby volcanic centers, reworked by water, and deposited in the basin.

  8. A 3-D Model of Stacked Thrusts in the Sevier Thrust Belt, Eastern Idaho

    NASA Astrophysics Data System (ADS)

    Clayton, R. W.; Clayton, S. R.

    2014-12-01

    Using published and new geologic map data and two exploratory wells for control, we constructed a three-dimensional geological model of the Pine Creek area in the Big Hole Mountains of eastern Idaho, where stacked Sevier thrust sheets are exposed at the surface. In this area, Cretaceous crustal shortening displaced and folded strata from Cambrian to Cretaceous in age. Using geologic map data as a primary input to a 3-D model presents a number of challenges, especially representing fault geometries at depth and maintaining strata thicknesses. The highly variable attitudes measured at the surface are also difficult to represent in a subsurface model because they require extensive extrapolation to depth. To overcome these challenges we EarthVision software, which has tools for model construction with minimal data inputs and uses a minimum tension algorithm to create geologically realistic surfaces. We also constructed two primary cross-sections to constrain strata and fault geometries according to structural principles, and used these to guide construction of fault and horizon surfaces. We then designated horizons with the best control as reference horizons to constrain strata geometries, and built the remaining horizons using isochores to add or subtract from those surfaces. The model shows classic flat-ramp thrust geometries as seen farther southeast in the Wyoming section of the thrust belt. The model also shows uniform southwestward tilting of faults and strata in the north end above younger thrusts, but strong effects from a duplex on a younger thrust fault encountered in the southern well, which rotated the strata and older faults above it.

  9. Recognition of Escherichia coli attTn7 by transposon Tn7: lack of specific sequence requirements at the point of Tn7 insertion.

    PubMed Central

    Gringauz, E; Orle, K A; Waddell, C S; Craig, N L

    1988-01-01

    Transposon Tn7 inserts at high frequency into a specific site in the Escherichia coli chromosome called attTn7. We show that the point of Tn7 insertion in attTn7 lies within the transcriptional terminator of the bacterial glmS gene. We have exploited the glmS transcription terminator to isolate mutants with altered sequences at the point of Tn7 insertion and have used these mutants to show that the nucleotide sequence at the point of Tn7 insertion is irrelevant to attTn7 target activity. Thus, the nucleotides which provide attTn7 target activity are distinct from the point of Tn7 insertion. We have also examined the effect of transcription on the capacity of attTn7 to act as a target for Tn7 transposition. Our results suggest that transcription of attTn7 does not modulate its Tn7 target activity. PMID:2836374

  10. 75 FR 52367 - Notice of Inventory Completion: Memphis Pink Palace Museum, Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... minimum of one individual were removed from the Pelegrin site, which is a component of the Carson Mounds... Hatchie Mound,'' Tipton County, TN, by Elbert L. Roper, an avocational archeologist. Mr. Roper excavated... the `Big Hatchie Country' and I think that `mound' just got added on. Certainly the bulk of his...

  11. Rapid Intradeformational Emplacement of the Big Hole Canyon Pluton Into the Sevier Fold-Thrust Belt, Southwest Montana.

    NASA Astrophysics Data System (ADS)

    Hespenheide, M. A.

    2002-12-01

    The Big Hole Canyon pluton (BHCp) is a Late Cretaceous pluton emplaced within the Sevier fold-and-thrust belt of the western North American Cordillera. The pluton is exposed over 60km2 and a thickness of ~1400m. Combined anisotropy of magnetic susceptibility (AMS), structural, and field studies document a clear pattern of magmatic flow radiating from at least three subvertical conduits <100m wide and ~300 to ~800m long. Interpreted flow plunges change rapidly to subhorizontal fabrics across the rest of the pluton, matching the expected pattern for laccolithic emplacement. Ascent conduits within the Big Hole Canyon pluton are coincident with the fold axis of an anticline above a thrust ramp, suggesting that the magma ascended up the fault of the fault-bend-fold. Geobarometry and stratigraphic reconstructions indicate an emplacement depth of approximately ~3km. Preliminary thermal modeling indicates that the BHCp was emplaced in 250,000 years, likely between periods of regional shortening deformation. Rapid magma ascent rates calculated by dike flow modeling and implied by entrained wall-rock xenoliths may indicate sequential magma injection into the pluton; an absence of chill margins between phases within the pluton indicates that sequential injections must have taken place quickly enough that the magmas did not have time to cool below the solidus temperature. The geometry and location of the BHCp suggest that magma used a pre-existing fault as a mechanical discontinuity for both ascent and emplacement. Continued intrusion of magma had a sufficient amount of driving pressure to stretch, shear, and lift the roof of the pluton. Detailed field mapping, structural studies, AMS, and thermobarometry indicate that the Late Cretaceous Big Hole Canyon pluton was emplaced as a laccolith at the top of a pre-existing fault-bend-fold in the frontal portion of the Sevier fold-thrust belt.

  12. ERDC/TN ANSRP-06-__ November 2006

    E-print Network

    : The Upper Mississippi River (UMR) system starts at the confluence of the Ohio River at Cairo, IllinoisERDC/TN ANSRP-06-__ November 2006 1 Life History Attributes of Asian Carps in the Upper Mississippi River System by James E. Garvey, Kelly L. DeGrandchamp, and Christopher J. Williamson INTRODUCTION

  13. Ignite Serves Home Town: Johnson City, TN

    E-print Network

    Wang, Xiaorui "Ray"

    advice for new students: Go to class, make new friends, and enjoy college it goes by fast. Favorite UT's so fun seeing everyone on campus show their UT pride. Home Town: Memphis, TN High School: St sushi, getting dressed up and going out, drinking Starbucks, a White Chocolate Mocha to be exact Kiah

  14. 78 FR 48762 - Tennessee Disaster #TN-00076

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-09

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00076 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... Application Deadline Date: 05/02/2014. ADDRESSES: Submit completed loan applications to: U.S. Small...

  15. Neotectonics of the Sevier Desert basin, Utah as seen through the lens of multi-scale geophysical investigations

    NASA Astrophysics Data System (ADS)

    McBride, John H.; Nelson, Stephen T.; Heiner, Brandon D.; Tingey, David G.; Morris, Thomas H.; Rey, Kevin A.

    2015-07-01

    Geophysical surveys conducted in the Clear Lake fault zone (Utah) constrain the interpretation of neotectonics and volcanism along a portion of the Colorado Plateau-Basin-and-Range Transition Zone. The fault zone lies within the Sevier Desert, a region marked by numerous Quaternary fault scarps and bimodal volcanism. Lavas as young as 660 a B.P. are usually cut by faults, and many scarps have significant offsets implying the potential for M 7 + earthquakes. The range of scarp heights and uncertainty in scarp-magnitude regressions suggest variations on the order of 0.75 moment magnitude units. In order to characterize the range of deformation styles in the Clear Lake fault zone, three fault scarps were investigated for their patterns of shallow (< 1 km depth) deformation. A suite of methods, including seismic P- and SH-wave surveys, ground-penetrating radar, auger holes, and surface geological mapping produces a more complete view of near-surface Quaternary deformation. Sub-surface faults are expressed as scarps at the ground surface, but with differing tectonic styles: (1) growth strata in the hanging wall of a fault imply aseismic creep; (2) buried lavas draped over buried paleo-scarps indicate multiple slip events; and (3) a complex fault zone is expressed as ramps that step-over from one scarp to another. On a larger scale, the results bear directly on estimating seismic and volcanic hazards. In one locality, evidence of aseismic creep suggests hazard is lower than that inferred from the exposed scarp height or length. By contrast, multiple slip events at other locations indicate that hazards could be underestimated if scarps are assumed to represent single slip events. Finally, the intimate spatial association of Quaternary volcanic vents and faults implies that volcanic and seismic hazards are interrelated, both resulting from crustal extension where eruptive events exploited faults as conduits in the shallow crust.

  16. ATP-dependent specific binding of Tn3 transposase to Tn3 inverted repeats

    NASA Astrophysics Data System (ADS)

    Wishart, W. L.; Broach, J. R.; Ohtsubo, E.

    1985-04-01

    Transposons are discrete segments of DNA which are capable of moving from one site in a genome to many different sites1,2. Tn3 is a prokaryotic transposon which is 4,957 base pairs (bp) long and encodes a transposase protein which is essential for transposition3-7. We report here a simple method for purifying Tn3 transposase and demonstrate that the transposase protein binds specifically to the ends of the Tn3 transposon in an ATP-dependent manner. The transposase protein binds to linear double-stranded DNA both nonspecifically and specifically; the nonspecific DNA binding activity is sensitive to challenge with heparin. Site-specific DNA binding to the ends (inverted repeats) of Tn3 is observed only when binding is performed in the presence of ATP; this ATP-dependent site-specific DNA binding activity is resistant to heparin challenge. Our results indicate that ATP qualitatively alters the DNA binding activity of the transposase protein so that the protein is able to bind specifically to the ends of the Tn3 transposon.

  17. An Early Cretaceous garnet pressure-temperature path recording synconvergent burial and exhumation from the hinterland of the Sevier orogenic belt, Albion Mountains, Idaho

    NASA Astrophysics Data System (ADS)

    Kelly, Eric D.; Hoisch, Thomas D.; Wells, Michael L.; Vervoort, Jeffrey D.; Beyene, Mengesha A.

    2015-08-01

    Rocks may undergo complex pressure-temperature ( P- T) histories during orogenesis in response to alternating episodes of synconvergent burial and exhumation. In this study, chemical zoning in garnets combined with textural and chemical evidence from the schist of Willow Creek in the Albion Mountains of south-central Idaho (USA), reveals a complex P- T path during the early stages of Sevier orogenesis. The distribution of quartz inclusions combined with internal resorption features establishes a hiatus in garnet growth. Chemical zoning was simulated using a G-minimization approach to yield a P- T path consisting of three distinct pressure changes during increasing temperature, defining an "N" shape. Lu-Hf isochron ages from multiple garnet fractions and whole-rock analyses in two samples are 132.1 2.4 and 138.7 3.5 Ma. The samples were collected from the hanging wall of the Basin-Elba thrust fault and yielded results similar to those previously obtained from the footwall. This leads to several conclusions: (1) Both the hanging wall and footwall experienced the same metamorphic event, (2) the paths document a previously unrecognized crustal thickening and synorogenic extension cycle that fills an important time gap in the shortening history of the Sevier retroarc, suggesting progressive eastward growth of the orogen rather than a two-stage history, and (3) episodes of extensional exhumation during protracted convergent orogenesis are increasingly well recognized and highlight the dynamic behavior of orogenic belts.

  18. The Drinfeld double gl(n) ? tn

    NASA Astrophysics Data System (ADS)

    Ballesteros, A.; Celeghini, E.; del Olmo, M. A.

    2006-07-01

    We present a self-dual Drinfeld double structure underlying the An series of simple Lie algebras. Such double is constructed through a central extension tn of gl(n), and is obtained by pairing two disjoint solvable subalgebras coming from positive and negative roots. The Cartan-Weyl basis of gl(n) is shown to be completely determined by the compatibility conditions in the double. A natural Lie bialgebra structure on gl(n) is obtained that offers a new perspective for the construction of its quantum deformations.

  19. Licensing the TN-40 at Prairie Island

    SciTech Connect

    Kapitz, J.; McCarten, L. )

    1991-11-01

    Northern States Power Company (SNP) is currently in the process of licensing an independent spent-fuel storage installation (ISFSI) at its Prairie Island nuclear generating plant. The proposed facility would utilize the TN-40 dry-metal storage casks, each holding 40 Prairie Island spent-fuel assemblies. The ISFSI consists of two concrete pads with a combined capacity of 48 casks for a total capacity of 1,920 fuel assemblies. In addition to a 10CRF72 license from the US Nuclear Regulatory Commission (NRC), a certificate of need from the Minnesota Public Utilities Commission is required by state law.

  20. 14. Roaring Fork Motor Nature Trail, Place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  1. Challenges in Antibody Development against Tn and Sialyl-Tn Antigens

    PubMed Central

    Loureiro, Liliana R.; Carrascal, Mylne A.; Barbas, Ana; Ramalho, Jos S.; Novo, Carlos; Delannoy, Philippe; Videira, Paula A.

    2015-01-01

    The carbohydrate antigens Tn and sialyl-Tn (STn) are expressed in most carcinomas and usually absent in healthy tissues. These antigens have been correlated with cancer progression and poor prognosis, and associated with immunosuppressive microenvironment. Presently they are used in clinical trials as therapeutic vaccination, but with limited success due to their low immunogenicity. Alternatively, anti-Tn and/or STn antibodies may be used to harness the immune system against tumor cells. Whilst the development of antibodies against these antigens had a boost two decades ago for diagnostic use, so far no such antibody entered into clinical trials. Possible limitations are the low specificity and efficiency of existing antibodies and that novel antibodies are still necessary. The vast array of methodologies available today will allow rapid antibody development and novel formats. Following the advent of hybridoma technology, the immortalization of human B cells became a methodology to obtain human monoclonal antibodies with better specificity. Advances in molecular biology including phage display technology for high throughput screening, transgenic mice and more recently molecularly engineered antibodies enhanced the field of antibody production. The development of novel antibodies against Tn and STn taking advantage of innovative technologies and engineering techniques may result in innovative therapeutic antibodies for cancer treatment. PMID:26270678

  2. Structural and microstructural evolution of the Rattlesnake Mountain Anticline (Wyoming, USA): New insights into the Sevier and Laramide orogenic stress build-up in the Bighorn Basin

    NASA Astrophysics Data System (ADS)

    Beaudoin, Nicolas; Leprtre, Rmi; Bellahsen, Nicolas; Lacombe, Olivier; Amrouch, Khalid; Callot, Jean-Paul; Emmanuel, Laurent; Daniel, Jean-Marc

    2012-11-01

    The Rocky Mountains in western US provide among the best examples of thick-skinned tectonics: following a period of thin-skinned tectonics related to the Sevier orogeny, the compressional reactivation of basement faults gave birth to the so-called Laramide uplifts/arches. The Bighorn basin, located in Wyoming, is therefore a key place to study the transition from thin- to thick-skinned tectonics in orogenic forelands, especially in terms of microstructural and stress/strain evolution. Our study focuses on a classic Laramide structure: the Rattlesnake Mountain Anticline (RMA, Wyoming, USA), a basement-cored anticline located in the western part of the Bighorn basin. Stress and strain evolution analysis in folded sedimentary layers and underlying faulted basement rocks were performed on the basis of combined analyses of fractures, fault-slip data and calcite twinning paleopiezometry. Most of the fractures are related to three main tectonic events: the Sevier thin-skinned contraction, the Laramide thick-skinned contraction, and the Basin and Range extension. Serial balanced cross-sections of RMA and displacement profiles suggest that all thrust faults were coeval, evidencing strain distribution in the basement during faulting. The comparison of RMA with another structure located in the eastern edge of the Bighorn basin, i.e. the Sheep Mountain Anticline (SMA), allows to propose a conceptual model for the geometric and kinematic evolution of Laramide-related basement-cored anticlines. Finally, the stress evolution is reconstructed at both the fold scale and the basin scale. We show that the evolution of stress trends and magnitudes was quite similar in both structures (RMA and SMA) during Laramide times (thick-skinned tectonics), in spite of different stress regimes. During Sevier (thin-skinned tectonics) and post-Laramide times, stress trends and fracture patterns were different in these two structures. These results suggest that the distance to the orogenic front influenced the fracture patterns but not the foreland stress magnitudes, which were likely controlled by the structural style.

  3. A review of the TN theory and its cousins

    NASA Astrophysics Data System (ADS)

    Tachikawa, Yuji

    2015-11-01

    The T_N theory is a four-dimensional N = 2 superconformal field theory that has played a central role in the analysis of supersymmetric dualities in the last few years. The aim of this review is to collect known properties of the T_N theory and its cousins in one place as a quick reference.

  4. 77 FR 61653 - Notice of Intent To Rule on Request To Release Airport Property at the Smyrna-Rutherford County...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-10

    ... Smyrna-Rutherford County Airport, Smyrna, TN AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... the release of land at the Smyrna-Rutherford County Airport, Smyrna, Tennessee. This property... review at the Smyrna--Rutherford County Airport, 278 Doug Warpoole Road, Smyrna, Tennessee 37167 and...

  5. Insertion sites and the terminal nucleotide sequences of the Tn4 transposon.

    PubMed Central

    Hyde, D R; Tu, C P

    1982-01-01

    The nucleotide sequences at the ends of the Tn4 transposon (mercury spectinomycin and sulfonamide resistance) have been determined. They are inverted repeated sequences of 38 nucleotides with three mismatched base pairs. These sequences are strongly homologous with the terminal sequences of Tn501 (mercury resistance) but less so with those of Tn3 (ampicillin resistance). The Tn4 transposon generates pentanucleotide members (Tn3, Tn1000, Tn501, Tn551, IS2) with the exception of Tn1721 and bacteriophage Mu. Among the three Tn4 insertion sites examined here, two of them occurred near a nonanucleotide sequence in perfect homology with part of the terminal inverted-repeat sequence of Tn4 and the third insertion occurred near a sequence of partial homology to one end of Tn4. All three insertions were in the same orientation such that IRb is proximal to its homologous sequence on the recipient DNA. Images PMID:6287429

  6. 75 FR 27008 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: Small Business Administration. ACTION: Amendment 3. SUMMARY: This... declaration remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James...

  7. 75 FR 27010 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 2. SUMMARY: This... Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance. BILLING...

  8. 75 FR 35103 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: Small Business Administration. ACTION: Amendment 7. SUMMARY: This... remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E....

  9. 75 FR 27009 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 1. SUMMARY: This... remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E....

  10. 75 FR 27008 - TENNESSEE Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION TENNESSEE Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 3. SUMMARY: This... unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera,...

  11. 75 FR 35103 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 8. SUMMARY: This... Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance....

  12. Deformation along a salient-transverse zone junction: An example from the Leamington transverse zone, Utah, Sevier fold-thrust belt (USA)

    NASA Astrophysics Data System (ADS)

    Ismat, Zeshan; Toeneboehn, Kevin

    2015-06-01

    Within fold-thrust belts, the junctions between salients and recesses may hold critical clues to the overall kinematic history of fold-thrust belts. The deformation history within these junctions is best preserved in areas where thrust sheets extend from a salient through an adjacent recess. We examine one such junction within the Sevier fold-thrust belt (western United States) along the Leamington transverse zone, northern Utah. The Canyon Range thrust sheet can be traced continuously from the Leamington transverse zone to its adjacent salient to the south, the Central Utah segment. Deformation within the Canyon Range thrust sheet took place by faulting and cataclastic flow. Analyses of these fault networks preserved throughout the Canyon Range thrust sheet are used to develop a kinematic history of the Leamington transverse zone. Field data is supplemented by analog sandbox experiments. This study suggests that, in detail, deformation within the overlying thrust sheet may not directly reflect the underlying basement structure. Moreover, these junctions may contain several types of accommodating structures that helped to maintain critical-taper and that serve as potential targets for natural resource exploration.

  13. Creation of Staphylococcal Mutant Libraries Using Transposon Tn917.

    PubMed

    Rice, Kelly C

    2016-01-01

    Non-directed mutagenesis of the staphylococcal genome is a global approach that can be used to identify the genetic basis for phenotypes of interest as well as for identifying regulators of gene expression. One such approach that has been widely used in the study of S. aureus and S. epidermidis is transposon Tn917 mutagenesis to generate random libraries of mutants. This chapter describes the use of plasmid pLTV1 (containing Tn917-lac) to generate Tn917 transposon mutants. Through the use of temperature manipulation and antibiotic selection, staphylococcal strains harboring this plasmid can be effectively mutagenized to create random libraries amenable to subsequent phenotypic screening and identification of transposon insertion sites. PMID:25646610

  14. 40 CFR 81.343 - Tennessee.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... affecting 81.343, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... Robertson County X Rutherford County X Scott County X Sequatchie County X Sevier County X Shelby County X... Rutherford County X Scott County X Sequatchie County X Sevier County X Shelby County X Smith County X...

  15. 77 FR 40489 - Amendment of Class E Airspace; Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-10

    ... amend Class E airspace at Memphis, TN (77 FR 17360). Interested parties were invited to participate in... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a Regulatory Evaluation...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p....

  16. 78 FR 38555 - Amendment of Class E Airspace; Selmer, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-27

    ..., TN. (78 FR 16202). Interested parties were invited to participate in this rulemaking effort by... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a Regulatory Evaluation...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p....

  17. 76 FR 1512 - Amendment of Class E Airspace; Savannah, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... the surface, at Savannah, TN (75 FR 65584) Docket No. FAA-2010-1047. Interested parties were invited... 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034... continues to read as follows: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565,...

  18. 75 FR 27009 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: Small Business Administration. ACTION: Amendment 2. SUMMARY: This... 59008) James E. Rivera, Associate Administrator for Disaster Assistance. BILLING CODE 8025-01-P...

  19. 76 FR 48939 - Tennessee Disaster Number TN-00058

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00058 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY... Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance....

  20. 75 FR 27009 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Printing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY... 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance. BILLING CODE...

  1. The generalised Ramanujan-Nagell equation T.N. Shorey

    E-print Network

    Shorey, T.N.

    The generalised Ramanujan-Nagell equation T.N. Shorey Ramanujan considered the equation (1) x 2 + 7 = 2 n+2 in integers x > 0; n > 0: Ramanujan [11] observed in 1913 that the solutions of (1) are given and (1) is known as Ramanujan - Nagell equation. We shall consider a more general equation called

  2. PV SOLAR RESOURCE GUIDE FOR RURAL DISTRIBUTORS IN TN Introduction

    E-print Network

    McCarthy, John F.

    April 2013 Asa Roy PV SOLAR RESOURCE GUIDE FOR RURAL DISTRIBUTORS IN TN #12;1 Introduction experience with solar power. Research and interviews with the Tennessee's Green Power Providers Program, Green Power Providers Coordinators at several urban distributors, and solar installers in Tennessee

  3. 76 FR 36166 - Tennessee Disaster Number TN-00051

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ... From the Federal Register Online via the Government Printing Office U.S. SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4...: Submit completed loan applications to: U.S. Small Business Administration, Processing and...

  4. NASA TECHNICAL NOTE TN D-6738 iiiiiiiiii m

    E-print Network

    Rathbun, Julie A.

    THERMAL DESIGN OF CENTRAL STATION . THERMAL DESIGN OF EXPERIMENTS Passive Seismic Experiment LunarNASA TECHNICAL NOTE TN D-6738 iiiiiiiiii m n === r- AFWL APOLLO EXPERIENCE REPORT - THERMAL DESIGN !__ #12;KAFB, - 4. Title and Subtitle 5. Report Date APOLLO EXPERIENCE REPORT March 1972 THERMAL DESIGN

  5. * Middle Tennessee State University, Department of Physics and Astronomy, Murfreesboro, TN 37132 Vanderbilt University, Department of Chemistry, Nashville, TN 37235

    E-print Network

    Current silicon based solar cells are limited, not due to advances in technology, but due to the physics advantages over current silicon based solar cells. CdSe nanocrystals are facile and cost efficient to produce* Middle Tennessee State University, Department of Physics and Astronomy, Murfreesboro, TN 37132

  6. 78 FR 27029 - Modification of Class C Airspace; Nashville International Airport; TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ...No. 12-AWA-7] Modification of Class C Airspace; Nashville International Airport...Nashville International Airport, TN, Class C airspace area by removing a cutout from the...Nashville International Airport, TN, Class C airspace area (78 FR 6257)....

  7. The Lower Sevier River Basin Crop Monitor and Forecast Decision Support System: Exploiting Landsat Imagery to Provide Continuous Information to Farmers and Water Managers

    NASA Astrophysics Data System (ADS)

    Torres-Rua, A. F.; Walker, W. R.; McKee, M.

    2013-12-01

    The last century has seen a large number of innovations in agriculture such as better policies for water control and management, upgraded water conveyance, irrigation, distribution, and monitoring systems, and better weather forecasting products. In spite of this, irrigation management and irrigation water deliveries by farmers/water managers is still based on factors like water share amounts, tradition, and past experience on irrigation. These factors are not necessarily related to the actual crop water use; they are followed because of the absence of related information provided in a timely manner at an affordable cost. Thus, it is necessary to develop means to deliver continuous and personalized information about crop water requirements to water users/managers at the field and irrigation system levels so managers at these levels can better quantify the required versus available water for irrigation during the irrigation season. This study presents a new decision support system (DSS) platform that addresses the absence of information on actual crop water requirements and crop performance by providing continuous updated farm-based crop water use along with other farm performance indicators such as crop yield and farm management to irrigators and water managers. This DSS exploits the periodicity of the Landsat Satellite Mission (8 to 16 days, depending on the period of interest) to provide remote monitoring at the individual field and irrigation system levels. The Landsat satellite images are converted into information about crop water use, yield performance and field management through application of state-of-the-art semi-physical and statistical algorithms that provide this information at a pixel basis that are ultimately aggregated to field and irrigation system levels. A version of the DSS has been implemented for the agricultural lands in the Lower Sevier River, Utah, and has been operational since the beginning of the 2013 irrigation season. The main goal of this DSS implementation is to provide continuous and personalized information to farmers and water managers regarding crops in fields and the irrigation delivery system throughout the irrigation season so that decisions related to agricultural water use can result in water savings while not diminishing crop yields.

  8. A comparison of Anisotropy of Magnetic Susceptibility Studies to Clast Based Strain Analysis in Sandstones, From The Outer Margin Of The Sevier Orogenic Foreland, Western Wyoming.

    NASA Astrophysics Data System (ADS)

    Mc Carthy, Dave; Meere, Pat; Petronis, Mike; Mulchrone, Kieran

    2013-04-01

    The Cordilleran Mountain Belt of North America is one of the world's classic foreland fold and thrust belts. The Sevier Belt represents the thin skinned front of this orogeny, consisting of thrust faults and folds that shortened and transported sequences of Devonian to Cretaceous strata eastward. There is a general increase in deformation westwards which provides an ideal geological setting to explore the potential link between Anisotropy of Magnetic Susceptibility (AMS) and results from clast based strain analyses of sandstones. Studies attempting to define the relationship between AMS and finite strain have been in vogue since the link between layer parallel shortening and AMS was first established. The understanding of this relationship, despite proven strong correlations between the AMS tensors and tectonic directions, is complicated by competing sub-fabrics, as well as the various magnetic properties of the minerals contributing to the AMS fabric. It has become very clear that the sensitivity of AMS is capable of detecting incipient tectonic fabrics, <5% shortening, which is typically outside the range of most dedicated strain analyses. Despite this, there has been little published research into the relationship between the classic fabric analysis techniques or strain estimate methods and AMS. This may be due to the particularly laborious task of calculating strain estimates using large marker populations and/or the pitfalls of trying to relate the magnitudes of the magnetic ellipse to the magnitudes of the strain ellipse. Rather than trying to estimate finite strain directly from the AMS ellipsoid, we are using the ability of AMS to accurately and quickly qualify the petrofabric and determine the origin of that fabric (i.e., whether it is purely sedimentary, composite bedding/tectonic or dominantly tectonic etc.). Where as most methods of estimating strain have poor accuracy constraints in low strain regimes. In an attempt to account for this AMS is being utilised as a control for the sensitivity limits for these strain analysis methods, this is in part due to low sample counts, subjectivity and human error. This research uses recently developed and remodified strain analysis methods that are being utilised in a Mathematica environment. The use of Mathematica allows for objective, swift, reproducible and automated calculation of a suite of strain estimate techniques, such as the Fry method, a variation of Ramsay's Nearest Neighbour Method and the Mean Radial Length Method.

  9. Lightcurve Analysis of Near-Earth Asteroid 2010 TN54

    NASA Astrophysics Data System (ADS)

    Warner, Brian D.; Benishek, Vladimir; Ferrero, Andrea

    2014-01-01

    CCD photometry observations of the near-Earth asteroid (NEA) 2010 TN 54 indicate a period of either 6.14 h or 12.12 h, depending on whether a monomodal or bimodal lightcurve is adopted. The amplitude was only 0.07 0.01 mag, which - along with the period being nearly commensurate with an Earth day - made finding a definitive solution difficult, despite being observed from locations in North America and Europe.

  10. Characterization of Tn6238 with a new allele of aac(6')-Ib-cr.

    PubMed

    Quiroga, Mara P; Orman, Betina; Errecalde, Laura; Kaufman, Sara; Centrn, Daniela

    2015-05-01

    Here, we report that the genetic structure of Tn1331 remained conserved in Argentina from 1989 to 2013 (72 of 73 isolates), with the exception being the plasmid-borne Tn1331-like transposon Tn6238 containing a new aac(6')-Ib-cr allele recovered from a colistin-resistant Klebsiella pneumoniae clinical isolate. A bioinformatic analysis of aac(6')-Ib-like gene cassettes suggests that this new aac(6')-Ib-cr allele emerged through mutation or homologous recombination in the Tn1331 genetic platform. Tn6238 is a novel platform for the dissemination of aminoglycoside and fluoroquinolone resistance determinants. PMID:25691640

  11. 75 FR 32201 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ...species of mammals, fish, reptiles, and amphibians. Background The CCP Process The National...for non-game mammals, reptiles, amphibians and invertebrates. Similarly, within...for non-game mammals, reptiles, amphibians and invertebrates. Similarly,...

  12. 76 FR 5194 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN; Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ...birds, and 280 species of mammals, fish, reptiles, and amphibians. We announce our decision and the availability of the final...implement baseline inventories for non-game mammals, reptiles, amphibians, fish, and invertebrates. We will also consider...

  13. 75 FR 6257 - Watts Bar Reservoir Land Management Plan, Loudon, Meigs, Rhea, and Roane Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ...AUTHORITY Watts Bar Reservoir Land Management Plan, Loudon, Meigs, Rhea...has updated its 1988 land management plan (1988 Plan) for 16...thoroughfares and shorelines. Best management practices would be used on...erosion-control work would utilize seed mixes comprised of...

  14. 77 FR 24200 - American Drum & Pallet, Memphis, Shelby County, TN; Notice of Settlement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-23

    ...Painter.Paula@epa.gov. FOR FURTHER INFORMATION CONTACT: Paula V. Painter at 404/562-8887. Dated: April 9, 2012. Anita L. Davis, Chief, Superfund Enforcement & Information Management Branch, Superfund Division. [FR Doc. 2012-9696...

  15. 76 FR 5194 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN; Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ... (73 FR 17994). On December 28, 1945, President Harry S. Truman signed Executive Order No. 9670... period via Federal Register notice on June 7, 2010 (75 FR 32201). We received 43 comments on the Draft... mammals, fish, reptiles, and amphibians. We announce our decision and the availability of the final...

  16. 75 FR 32201 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ... started the process through a notice in the Federal Register on April 2, 2008 (73 FR 17994). On December... amphibians. Background The CCP Process The National Wildlife Refuge System Administration Act of 1966 (16 U.S... more baseline inventories for non-game mammals, reptiles, amphibians and invertebrates....

  17. 8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY VIEW OF ABUTMENT SUPPORT INTO THE GROUND - Lost Creek Road Bridge, Spanning Sevier River at County Road 243, 2 miles east of Aurora, Aurora, Sevier County, UT

  18. 78 FR 7993 - Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-05

    ...of Class E Airspace; Tri-City, TN AGENCY: Federal Aviation...Class E airspace in the Tri-Cities, TN, area, to accommodate the new Area Navigation (RNAV) Global Positioning System (GPS) Standard...Approach Procedures at Tri-Cities Regional Airport. This...

  19. 40 CFR 81.94 - Shreveport-Texarkana-Tyler Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...Little River County, Miller County, Sevier County. In the State of Louisiana...Parish. In the State of Oklahoma: McCurtain County. In the State of Texas...Camp County, Cass County, Cherokee County, Delta County,...

  20. Tn5 Transposase with an Altered Specificity for Transposon Ends

    PubMed Central

    Naumann, Todd A.; Reznikoff, William S.

    2002-01-01

    Tn5 is a composite bacterial transposon that encodes a protein, transposase (Tnp), required for movement of the transposon. The initial step in the transposition pathway involves specific binding of Tnp to 19-bp end recognition sequences. Tn5 contains two different specific end sequences, termed outside end (OE) and inside end (IE). In Escherichia coli, IE is methylated by Dam methylase (IEME). This methylation greatly inhibits recognition by Tnp and greatly reduces the ability of transposase to facilitate movement of IE defined transposons. Through use of a combinatorial random mutagenesis technique (DNA shuffling), we have isolated an IEME-specific hyperactive form of Tnp, Tnp sC7v.2.0, that is able to promote high levels of transposition of IEME defined transposons in vivo and in vitro while functioning at wild-type levels with OE transposons. This protein contains a critical glutamate-to-valine mutation at amino acid 58 that is responsible for this change in end specificity. PMID:11741865

  1. Doc: VIR-LES-TN-2338 Date: 28/10/2015

    E-print Network

    Meyer-Vernet, Nicole

    VIRTIS Doc: VIR-LES-TN-2338 Issue: 0.6 Date: 28/10/2015 Page: 1 VIRTIS Rosetta geometry files VIR/10/2015 Checked by F. Capaccioni Approved by Authorized by #12;VIRTIS Doc: VIR-LES-TN-2338 Issue: 0.6 Date: 28 .....................................................................................................20 #12;VIRTIS Doc: VIR-LES-TN-2338 Issue: 0.6 Date: 28/10/2015 Page: 3 DOCUMENT CHANGE RECORD Issue

  2. BWR - Spent Fuel Transport and Storage with the TN{sup TM}9/4 and TN{sup TM}24BH Casks

    SciTech Connect

    Wattez, L.; Marguerat, Y.; Hoesli, C.

    2006-07-01

    The Swiss Nuclear Utilities have started in 2001 to store spent fuel in dry metallic dual-purpose casks at ZWILAG, the Swiss interim storage facility. BKW FMB Energy Ltd., the Muehleberg Nuclear Power Plant owner, is involved in this process and has elected to store its BWR spent fuel in a new high capacity dual-purpose cask, the TNeTeM24BH from the COGEMA Logistics/TRANSNUCLEAR TN{sup TM}24 family. The Muehleberg BWR spent fuels are transported by road in a medium size shuttle transport cask and then transferred to a heavy transport/storage cask (dry transfer) in the hot cell of ZWILAG site. For that purpose, COGEMA Logistics designed and supplied: - Two shuttle casks, TN{sup TM}9/4, mainly devoted to transport of spent fuel from Muehleberg NPP to ZWILAG. Licensed according to IAEA 1996, the TN{sup TM}9/4 is a 40 ton transport cask, for 7 BWR high bum-up spent fuel assemblies. - A series of new high capacity dual-purpose casks, TN{sup TM}24BH, holding 69 BWR spent fuels. Two transport campaigns took place in 2003 and 2004. For each campaign, ten TN{sup TM}9/4 round trips are performed, and one TN{sup TM}24BH is loaded. 5 additional TN{sup TM}24BH are being manufactured for BKW, and the next transport campaigns are scheduled from 2006. The TN{sup TM}24BH high capacity dual purpose cask and the TN{sup TM}9/4 transport cask characteristics and capabilities will then be detailed. (authors)

  3. Target Immunity of the Tn3-Family Transposon Tn4430 Requires Specific Interactions between the Transposase and the Terminal Inverted Repeats of the Transposon ?

    PubMed Central

    Nicolas, Emilien; Lambin, Michal; Hallet, Bernard

    2010-01-01

    Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo and remained responsive to wild-type immunity conferred by the Tn4430 inverted repeat end. Intriguingly, the presence of lacO repeats within the target was found to strongly influence target site selection by Tn4430, but in a LacI-independent manner. PMID:20562304

  4. A bifunctional DNA binding region in Tn5 transposase

    PubMed Central

    Gradman, Richard J; Ptacin, Jerod L; Bhasin, Archna; Reznikoff, William S; Goryshin, Igor Y

    2008-01-01

    Tn5 transposition is a complicated process that requires the formation of a highly ordered proteinDNA structure, a synaptic complex, to catalyse the movement of a sequence of DNA (transposon) into a target DNA. Much is known about the structure of the synaptic complex and the positioning of proteinDNA contacts, although many proteinDNA contacts remain largely unstudied. In particular, there is little evidence for the positioning of donor DNA and target DNA. In this communication, we describe the isolation and analysis of mutant transposases that have, for the first time, provided genetic and biochemical evidence for the stage-specific positioning of both donor and target DNAs within the synaptic complex. Furthermore, we have provided evidence that some of the amino acids that contact donor DNA also contact target DNA, and therefore suggest that these amino acids help define a bifunctional DNA binding region responsible for these two transposaseDNA binding events. PMID:18086215

  5. Genetic Diversity of Tn916-Related Transposons among Drug-Resistant Streptococcus pneumoniae Isolates Colonizing Healthy Children in Venezuela?

    PubMed Central

    Quintero, Beatrz; Araque, Mara; van der Gaast-de Jongh, Christa; Hermans, Peter W. M.

    2011-01-01

    Among a collection of 48 multidrug-resistant pneumococcal strains colonizing healthy children in a small municipality of Mrida, Venezuela, we identified sequence types (STs) related to a variety of internationally spreading drug-resistant clones, as well as ST135, thus far isolated only in Europe. The clones invariably harbored one or more of the Tn916-related transposons Tn3872, Tn5253, Tn6002, Tn2009, and Tn2010. Finally, our data suggest both structural rearrangements in certain transposons and occurrence of novel transposable elements. PMID:21788464

  6. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ...South Fork National Recreation Area, TN/KY AGENCY: National Park...South Fork National Recreation Area, TN/KY...SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby...South Fork National Recreation Area, Tennessee and Kentucky,...

  7. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... contract for Big South Fork National Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public... National Park Service Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY... contract for the conduct of certain visitor services within Big South Fork National Recreation...

  8. Mutagenesis by insertion of drug resistance transposon Tn7 into a vibrio species.

    PubMed Central

    Thomson, J A; Hendson, M; Magnes, R M

    1981-01-01

    A halotolerant, collagenolytic strain of Vibrio sp. was conjugated with an Escherichia coli strain carrying plasmid RP4. The plasmid was transferred to and maintained in the Vibrio and could be subsequently transferred in matings to suitably marked stains of the same species. After conjugation with an E. coli carrying the cointegrate plasmid RP4::Mu cts61::Tn7, Vibrio transconjugants were selected that carried Tn7 inserted into the bacterial chromosome. A large proportion of these transconjugants were auxotrophic, showing that plasmid suicide by Mu can be used to isolate Tn7-derived mutants in Vibrio. Approximately half of the auxotrophs isolate Tn7-derived mutants in Vibrio. Approximately half of the auxotrophs isolated were ilv mutants, all of which exhibited the same phenotype. Thus, although Tn7 insertion can induce auxotrophy, including trp, thy, his and ura, in Vibrio, there does appear to be a hot spot for integration in the ilv operon. PMID:6270064

  9. Spectroscopic and ITC study of the conformational change upon Ca{sup 2+}-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle

    SciTech Connect

    Yumoto, Fumiaki; Tanaka, Hiroyuki; Nagata, Koji; Miyauchi, Yumiko; Miyakawa, Takuya; Ojima, Takao; Tanokura, Masaru

    2008-04-25

    Akazara scallop (Chlamys nipponensis akazara) troponin C (TnC) of striated adductor muscle binds only one Ca{sup 2+} ion at the C-terminal EF-hand motif (Site IV), but it works as the Ca{sup 2+}-dependent regulator in adductor muscle contraction. In addition, the scallop troponin (Tn) has been thought to regulate muscle contraction via activating mechanisms that involve the region spanning from the TnC C-lobe (C-lobe) binding site to the inhibitory region of the TnI, and no alternative binding of the TnI C-terminal region to TnC because of no similarity between second TnC-binding regions of vertebrate and the scallop TnIs. To clarify the Ca{sup 2+}-regulatory mechanism of muscle contraction by scallop Tn, we have analyzed the Ca{sup 2+}-binding properties of the complex of TnC C-lobe and TnI peptide, and their interaction using isothermal titration microcalorimetry, nuclear magnetic resonance, circular dichroism, and gel filtration chromatography. The results showed that single Ca{sup 2+}-binding to the Site IV leads to a structural transition not only in Site IV but also Site III through the structural network in the C-lobe of scallop TnC. We therefore assumed that the effect of Ca{sup 2+}-binding must lead to a change in the interaction mode between the C-lobe of TnC and the TnI peptide. The change should be the first event of the transmission of Ca{sup 2+} signal to TnI in Tn ternary complex.

  10. Center for Leadership and Service Community Partners American Cancer Society 871 Weisgarber Road, Knoxville, TN 37909 584-1668 Healthcare

    E-print Network

    McCarthy, John F.

    Populations Big Brothers Big Sisters 119 W. Summit Hill Dr. Suite 101 Knox, TN 37902 865-523-2179 x21 Literacy 1531 Dick Lonas Rd. Knox, TN 37909 865-602-2920 Women Fort Sanders Educational Development Center 501

  11. Tn6188 - A Novel Transposon in Listeria monocytogenes Responsible for Tolerance to Benzalkonium Chloride

    PubMed Central

    Muhterem-Uyar, Meryem; Zaiser, Andreas; Stessl, Beatrix; Guinane, Caitriona M.; Cotter, Paul D.; Wagner, Martin; Schmitz-Esser, Stephan

    2013-01-01

    Controlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S.) aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC) as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR) transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs) (28.5 4.7 mg/l) than strains without Tn6188 (14 3.2 mg/l). Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that ?qacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L. monocytogenes strains. PMID:24098567

  12. DNA Damage Differentially Activates Regional Chromosomal Loci for Tn7 Transposition in Escherichia coli

    PubMed Central

    Shi, Qiaojuan; Parks, Adam R.; Potter, Benjamin D.; Safir, Ilan J.; Luo, Yun; Forster, Brian M.; Peters, Joseph E.

    2008-01-01

    The bacterial transposon Tn7 recognizes replicating DNA as a target with a preference for the region where DNA replication terminates in the Escherichia coli chromosome. It was previously shown that DNA double-strand breaks in the chromosome stimulate Tn7 transposition where transposition events occur broadly around the point of the DNA break. We show that individual DNA breaks actually activate a series of small regional hotspots in the chromosome for Tn7 insertion. These hotspots are fixed and become active only when a DNA break occurs in the same region of the chromosome. We find that the distribution of insertions around the break is not explained by the exonuclease activity of RecBCD moving the position of the DNA break, and stimulation of Tn7 transposition is not dependent on RecBCD. We show that other forms of DNA damage, like exposure to UV light, mitomycin C, or phleomycin, also stimulate Tn7 transposition. However, inducing the SOS response does not stimulate transposition. Tn7 transposition is not dependent on any known specific pathway of replication fork reactivation as a means of recognizing DNA break repair. Our results are consistent with the idea that Tn7 recognizes DNA replication involved in DNA repair and reveals discrete regions of the chromosome that are differentially activated as transposition targets. PMID:18562643

  13. Brownfield to Brightfield Initiative in Oak Ridge, TN - 12346

    SciTech Connect

    Hough, Gil; Fairless, Chad

    2012-07-01

    Experience characterizing, permitting, and restoring 'Brownfield' sites-government or industrial sites with restricted future use due to the presence or potential presence of hazardous substances, pollutants, or contaminants-is being leveraged to identify opportunities for redevelopment into solar power generating facilities which, in this context, are called 'Brightfields'. Brownfield sites offer the expansive land necessary for large photovoltaic (PV) solar farms, but require an in-depth working knowledge of complicated regulatory restrictions and environmental constraints to develop them. As a part of the effort to identify opportunities for redevelopment of Brownfield sites for solar applications, a technical guide, was composed specifically for the development of solar generation on restricted use sites. The basis of the technical guide gives specific consideration to environmental requirements and installation methods breaking that into three areas for assessing: 1) levels of contamination, 2) ground penetration requirements, and 3) the requirements for aesthetics and maintenance. Brightfield projects are underway to support the technical guide and expand re-industrialization efforts for the former DOE Gaseous Diffusion Plant in Oak Ridge, TN. There are exciting opportunities to turn Brownfields into Brightfield solar energy solutions for meeting the future renewable energy needs of our country. Brownfields that offer the large surface area required for solar PV farms coupled with the technical guide for the installation of solar farms on restricted use sites supports efforts to develop the solar capacities and expertise to tap this future market. The initial projects designed following the technical guide will provide verification of the installation requirements and beneficial reuse of restricted use sites. (authors)

  14. Genetic analysis of the Tn21 mer operator-promoter.

    PubMed Central

    Park, S J; Wireman, J; Summers, A O

    1992-01-01

    The mercury resistance operon, mer, of the transposon Tn21 is transcribed from two overlapping divergent promoters: PR for the regulatory gene, merR, and PTPCAD for the structural genes, merTPCAD. Transcription of merTPCAD is repressed in the absence of Hg(II) and activated in the presence of Hg(II) by the regulatory protein, MerR. In addition, MerR represses its own expression regardless of the presence of Hg(II). MerR binds as a dimer to a single region of dyad symmetry lying between the -35 and -10 hexamers of PTPCAD. Analysis of the expression of transcriptional fusions to hydroxylamine- and oligonucleotide-generated mutants of this divergent operator-promoter region identified key bases involved in MerR-dependent repression of PTPCAD and of PR and in activation of PTPCAD. Six of the seven mutants affecting the palindromic region were altered in their ability to bind the MerR protein in vitro as measured by fragment retardation assays. These differences in in vitro MerR binding correlated well with the in vivo measurements of repression or of activation. Bases identified as functionally relevant by this genetic analysis coincide extensively with those previously identified as relevant via in vivo footprinting. Four major points emerge from this analysis: (i) transition and transversion mutations within the spacer between the -10 and -35 hexamers of PTPCAD generally have little effect on the MerR-independent (i.e., unregulated) expression of either promoter; (ii) alteration of certain bases in the MerR-binding dyad affects repression of PTPCAD differently than repression of PR; (iii) certain dyad changes can impair activation of PTPCAD more severely than repression of this promoter; and (iv) mutations in the -10 hexamer of PTPCAD which also effect PR expression define one of two potential -10 hexamers in PR as actually functional in vivo. Images PMID:1312997

  15. DRAFT LANDSAT DATA MOSAIC: MONTGOMERY COUNTY, TEXAS; HARRIS COUNTY, TEXAS; FORT BEND COUNTY, TEXAS; BRAZORIA COUNTY, TEXAS; GALVESTON COUNTY, TEXAS

    EPA Science Inventory

    This is a draft Landsat Data Mosaic, which contains remote sensing information for Montgomery County, Texas Harris County, Texas Fort Bend County, Texas Brazoria County, Texas Galveston County, and Texas Imagery dates on the following dates: October 6, 1999 and September 29, 200...

  16. Tn4563 transposition in Streptomyces coelicolor and its application to isolation of new morphological mutants.

    PubMed Central

    Schauer, A T; Nelson, A D; Daniel, J B

    1991-01-01

    The Tn3-like transposon Tn4556 (and its derivatives Tn4560 and Tn4563) has been used for insertion mapping of genetic loci cloned on plasmids, but it has been difficult to obtain chromosomal insertions, largely because of the lack of a strong selection against transposon donor molecules. In this communication, we report two efficient selection techniques for transposition and their use in the isolation of chromosomal insertion mutations. A number of independent Streptomyces coelicolor morphological mutants (bld and whi) were obtained. Two of the bld mutations were mapped to locations on the chromosome by SCP1-mediated conjugation; at least one mutation, bld-5m1, appears to define a novel locus involved in control of S. coelicolor morphogenesis and antibiotic production. Images PMID:1650343

  17. Asiago classification of ASASSN-15tn as a Type Ia SN

    NASA Astrophysics Data System (ADS)

    Tomasella, L.; Pastorello, A.; Benetti, S.; Cappellaro, E.; Elias-Rosa, N.; Ochner, P.; Tartaglia, L.; Terreran, G.; Turatto, M.

    2015-12-01

    The Asiago Transient Classification Program (Tomasella et al. 2014, AN, 335, 841) reports the spectroscopic classification of ASASSN-15tn discovered by All Sky Automated Survey for SuperNovae (ASAS-SN) in CGCG 496-51 (Atel#8376).

  18. A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production.

    PubMed

    Ben Salah, Riadh; Trabelsi, Imen; Ben Mansour, Riadh; Lassoued, Saloua; Chouayekh, Hichem; Bejar, Samir

    2012-08-01

    This study aimed to determine the probiotic potential of 100 strains of Lactic Acid Bacteria (LAB) isolated from different intestinal segments of indigenous poultry in Tunisia. The strains were submitted to a battery of standard tests and criteria commonly used for determining their probiotic properties and attributes. The findings revealed that 19 of the isolates exhibited antimicrobial activity against 4 pathogenic bacteria, and that 4 (TN1, TN8, TN7, and TN13) showed good resistance to pH 3 and 5% bovine bile. Three isolates, namely TN1, TN8, and TN13, showed sensitivity to several antibiotics and were, therefore, selected for further enzymatic activity assays. Two isolates, namely TN1 and TN8, showed high efficacy of adhesion to chicken enterocytes. The cytokines released after stimulation by the two isolates showed high anti-inflammatory profiles, with an increased rate of Interleukin-10 (IL-10) production for the TN8 strain. Showing the highest performance, TN8 was submitted to 16S rRNA gene sequencing, which revealed that the strain was of the species Lactobacillus plantarum. Overall, the findings indicate that the Lactobacilli from poultry intestine has a number of promising properties that make it candidate for application as a probiotic additive in poultry industry. PMID:22634330

  19. Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp.

    PubMed Central

    McHenney, M A; Baltz, R H

    1991-01-01

    Transposon Tn5096 was inserted into a derivative of the temperature-sensitive plasmid pMT660 containing the bacteriophage FP43 pac site. The resulting plasmid, pRHB126, was transduced by FP43 into several Streptomyces species. Tn5096 transposed from pRHB126 into different sites in the genomes of Streptomyces ambofaciens, Streptomyces cinnamonensis, Streptomyces coelicolor A3(2), Streptomyces fradiae, Streptomyces griseofuscus, and Streptomyces thermotolerans. Images PMID:1653214

  20. The Ramsey Numbers R(Tn, W6) for Yaojun CHEN

    E-print Network

    Zhang, Ke-Min

    loops. For two given graphs G1 and G2, the Ramsey number R(G1, G2) is the smallest positive integer n such that for any graph G of order n, either G contains G1 or G contains G2, where G is the complement of G. Let G a wheel of order m + 1. Baskoro et al. conjectured in [2] that if Tn is not a star, then R(Tn, Wm) = 2n

  1. Molecular Effects of Familial Hypertrophic Cardiomyopathy-Related Mutations in the TNT1 Domain of cTnT

    PubMed Central

    Manning, Edward P.; Tardiff, Jil C.; Schwartz, Steven D.

    2012-01-01

    Familial hypertrophic cardiomyopathy (FHC) is one of the most common genetic causes of heart disease. Approximately 15% of FHC-related mutations are found in cTnT [cardiac troponin (cTn) T]. Most of the cTnT FHC-related mutations are in or flanking the N-tail TNT1 domain that directly interacts with overlapping tropomyosin (Tm). We investigate two sets of cTnT mutations at opposite ends of TNT1, mutations in residue 92 in the TmTm overlap region of TNT1 and mutations in residues 160 and 163 in the C-terminal portion of TNT1 adjacent to the cTnT H1H2 linker. Though all the mutations are located within TNT1, they have widely different phenotypes clinically and biophysically. Using a complete atomistic model of the cTnTm complex, we identify mechanisms by which the effects of TNT1 mutations propagate to the cTn core and site II of cTnC, where calcium binding and dissociation occurs. We find that mutations in TNT1 alter the flexibility of TNT1, which is inversely proportional to the cooperativity of calcium activation of the thin filament. Further, we identify a pathway of propagation of structural and dynamic changes from TNT1 to site II of cTnC, including TNT1, cTnT linker, I-T arm, regulatory domain of cTnI, the D-E linker of cTnC, and site II cTnC. Mutationally induced changes at site II of cTnC alter calcium coordination that corresponds to biophysical measurements of calcium sensitivity. Finally, we compare this pathway of mutational propagation with that of the calcium activation of the thin filament and find that they are identical but opposite in direction. PMID:22579624

  2. Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903.

    PubMed Central

    Grindley, N D; Joyce, C M

    1980-01-01

    The kanamycin resistance transposon Tn903 consists of a unique region of about 1000 base pairs bounded by a pair of 1050-base-pair inverted repeat sequences. Each repeat contains two Pvu II endonuclease cleavage sites separated by 520 base pairs. We have constructed derivatives of Tn903 in which this 520-base-pair fragment is deleted from one or both repeats. Those derivatives that lack both 520-base-pair fragments cannot transpose, whereas those that lack just one remain transposition proficient. One such transposable derivative, Tn903 delta I, has been selected for further study. We have determined the sequence of the intact inverted repeat. The 18 base pairs at each end are identical and inverted relative to one another, a structure characteristic of insertion sequences. Additional experiments indicate that a single inverted repeat from Tn903 can, in fact, transpose; we propose that this element be called IS903. To correlate the DNA sequence with genetic activities, we have created mutations by inserting a 10-base-pair DNA fragment at several sites within the intact repeat of Tn903 delta 1, and we have examined the effect of such insertions on transposability. The results suggest that IS903 encodes a 307-amino-acid polypeptide (a "transposase") that is absolutely required for transposition of IS903 or Tn903. Images PMID:6261245

  3. A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2

    PubMed Central

    Gill, Sukhvinder; Krupovic, Mart; Desnoues, Nicole; Bguin, Pierre; Sezonov, Guennadi; Forterre, Patrick

    2014-01-01

    We report the characterization of a DNA primase/polymerase protein (PolpTN2) encoded by the pTN2 plasmid from Thermococcus nautilus. Sequence analysis revealed that this protein corresponds to a fusion between an N-terminal domain homologous to the small catalytic subunit PriS of heterodimeric archaeal and eukaryotic primases (AEP) and a C-terminal domain related to their large regulatory subunit PriL. This unique domain configuration is not found in other virus- and plasmid-encoded primases in which PriS-like domains are typically fused to different types of helicases. PolpTN2 exhibited primase, polymerase and nucleotidyl transferase activities and specifically incorporates dNTPs, to the exclusion of rNTPs. PolpTN2 could efficiently prime DNA synthesis by the T. nautilus PolB DNA polymerase, suggesting that it is used in vivo as a primase for pTN2 plasmid replication. The N-terminal PriS-like domain of PolpTN2 exhibited all activities of the full-length enzyme but was much less efficient in priming cellular DNA polymerases. Surprisingly, the N-terminal domain possesses reverse transcriptase activity. We speculate that this activity could reflect an ancestral function of AEP proteins in the transition from the RNA to the DNA world. PMID:24445805

  4. 78 FR 7993 - Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-05

    ... rulemaking (SNPRM) to amend Class D and Class E airspace in the Tri-Cities, TN, area (77 FR 59573). The SNPRM... read as follows: ] Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR... originally proposed in the NPRM of April 28, 2012 (77 FR 21505). Interested parties were invited...

  5. Tn6249, a new Tn6162 transposon derivative carrying a double-integron platform and involved with acquisition of the blaVIM-1 metallo-?-lactamase gene in Pseudomonas aeruginosa.

    PubMed

    Di Pilato, Vincenzo; Pollini, Simona; Rossolini, Gian Maria

    2015-03-01

    The In70.2 integron platform appears to be a conserved structure involved in the dissemination of the blaVIM-1 metallo-?-lactamase gene in Pseudomonas aeruginosa. The genetic context of the In70.2 integron platform from P. aeruginosa VR-143/97, the VIM-1-producing index strain isolated in Italy in 1997, was fully characterized by a next-generation sequencing approach refined by conventional sequencing. The In70.2 integron platform from VR-143/97 was found to be associated with a defective Tn402-like transposon inserted into the urf2 gene of a Tn3 family transposon of an original structure, named Tn6249, which also carried a partially deleted mer operon and an In90 integron platform in a tail-to-tail orientation. Tn6249 was inserted into a PACS171b-like genomic island, which was in turn inserted into the endA gene of the Pseudomonas chromosomal backbone. Tn6249 showed a similar structure and a conserved location with respect to that of Tn6060, a Tn3 family transposon associated with In70.2 and carrying a double-integron platform, which was detected in a VIM-1-producing P. aeruginosa strain isolated in Australia in 2008. Both Tn6249 and Tn6060 are apparently derived from Tn6162, a mercury resistance transposon carrying an integron platform, which was found in P. aeruginosa isolates from different geographic locations. The conservation of the genetic context of Tn6249 and Tn6060 suggests an in situ evolution of these elements after the insertion of a Tn6162-like ancestor into the PACS171b-like genomic island (GI) present in the genome of a successful widespread P. aeruginosa clonal lineage. PMID:25547348

  6. Appendix A - County Codes

    Cancer.gov

    January 1998 SEER Program Code Manual, 3 rd Edition A-1 APPENDIX A COUNTY CODES APPENDIX A COUNTY CODES A-2 SEER Program Code Manual, 3rd Edition January 1998 The following are the valid county codes for coding county of residence at diagnosis: Reference:

  7. A class 1 integron present in a human commensal has a hybrid transposition module compared to Tn402: evidence of interaction with mobile DNA from natural environments.

    PubMed

    Labbate, M; Roy Chowdhury, P; Stokes, H W

    2008-08-01

    In a survey of class 1 integrons from human stools, an unusual class 1 integron from a strain of Enterobacter cloacae was isolated and characterized in detail. Sequence analysis of a fosmid containing the class 1 integron revealed a complex set of transposons which included two Tn402-like transposons. One of these transposons, Tn6007, included a class 1 integron with two non-antibiotic-resistance-type gene cassettes and a complete transposition module. This tni module is a hybrid with a boundary within the res site compared to Tn402, implying that a site-specific recombination event generated either Tn6007 or Tn402. The second Tn402-like transposon, Tn6008, possesses neither a mer operon nor an integron, and most of its tni module has been deleted. Tn6007, Tn6008, and the 2,478 bases between them, collectively designated Tn6006, have transposed into a Tn5036/Tn3926-like transposon as a single unit. Tn6006, Tn6007, and Tn6008 could all transpose as discrete entities. Database analysis also revealed that a version of Tn6008 was present in the genome of Xanthomonas campestris pv. vesicatoria. Overall, the E. cloacae isolate further demonstrated that functional class 1 integrons/transposons are probably common in bacterial communities and have the potential to add substantially to the problem of multidrug-resistant nosocomial infections. PMID:18502858

  8. Purchasing in Texas Counties.

    E-print Network

    Hervey, E. J.; Bradshaw, H. C.

    1944-01-01

    were carefully studied. Following this detailed examination in four counties, purchasing pro- cedures were studied in considerably less detail in the following counties: Wharton, Waller, Liberty, Milam, Anderson, Harrison, Kaufman, Dallas, Tarrant... (After the program) 7,667 If the program had included a larger portion of the county road system, the saving would undoubtedly have been larger.' Harrison County In Harrison County in 1941, second grade (regular) gasoline was pur- chased in lots...

  9. [Variations of annual load of TN and TP in the deep bay watershed, Shenzhen].

    PubMed

    Zhao, Chen-Chen; Zhang, Shi-Yan; Mao, Xian-Zhong

    2014-11-01

    The empirical coefficient of sewage disposal, export coefficient model and mean concentration method were respectively used to estimate variations of annual load TN and TP from Shenzhen and Hong Kong areas in the Deep Bay Watershed from 1986 to 2011. The results showed that, the annual average loads of TN and TP were 10 388.2 t, 10 727.9 t, 10 937.3 t, and 2 694.5 t, 1 929.2 t, 1388.7 t, respectively in the whole watershed during three periods, 80s, 90s and years after 2000. With the rapid development of society, economy and the urbanization, annual pollution loading of TN and TP in Shenzhen area showed an obviously increase, 4373.6 t and 195.9 t, by 261.0% and 64.2% for point source, and 1067.2 t and 151.0 t, by 63.4% and 84.9% for non-point source, respectively. Non-point source with high pollution load was mainly caused by the expanding of land for construction and roads. The contribution ratios of TN and TP from Shenzhen area increased from 42.4% and 27.0% to 85.1% and 75.2%. Annual loads of TN and TP in Hong Kong area decreased 3 028.5 t and 1 031.5 t, by 66.3% and 79.0% reduced. PMID:25639083

  10. Distribution of the catabolic transposon Tn5271 in a groundwater bioremediation system.

    PubMed Central

    Wyndham, R C; Nakatsu, C; Peel, M; Cashore, A; Ng, J; Szilagyi, F

    1994-01-01

    The distribution of Tn5271-related DNA sequences in samples of groundwater and a groundwater bioremediation system at the Hyde Park (Niagara Falls, N.Y.) chemical landfill site was investigated. PCR amplification of target sequences within the cha genes of Tn5271 revealed similar sequences in the groundwater community and in samples from the sequencing batch reactors treating that groundwater. Cell dilution combined with PCR amplification indicated that cha sequences were carried in about 1 of 10 culturable bacteria from the treatment system. Characterization of isolates involved in chlorobenzoate and toluene biodegradation in the treatment system indicated that two phenotypic clusters, Alcaligenes faecalis type 2 and CDC group IVC-2, contained all of the Tn5271 probe-positive isolates from the community. These two groups differed phenotypically from recipient groups isolated following horizontal transfer of pBRC60 (Tn5271) in pristine freshwater microcosms. A genetic rearrangement in Tn5271 attributable to the intramolecular transposition of the flanking element IS1071R was detected in an isolate from the treatment system. Comparison of the structure of the intramolecular transposition derivative from groundwater isolate OCC13(pBRC13) with a laboratory-derived intramolecular transposition derivative of pBRC60 revealed similarities. The rearrangement was shown to increase the stability of the plasmid under starvation conditions. Images PMID:8117095

  11. TRANSIT--A Software Tool for Himar1 TnSeq Analysis.

    PubMed

    DeJesus, Michael A; Ambadipudi, Chaitra; Baker, Richard; Sassetti, Christopher; Ioerger, Thomas R

    2015-10-01

    TnSeq has become a popular technique for determining the essentiality of genomic regions in bacterial organisms. Several methods have been developed to analyze the wealth of data that has been obtained through TnSeq experiments. We developed a tool for analyzing Himar1 TnSeq data called TRANSIT. TRANSIT provides a graphical interface to three different statistical methods for analyzing TnSeq data. These methods cover a variety of approaches capable of identifying essential genes in individual datasets as well as comparative analysis between conditions. We demonstrate the utility of this software by analyzing TnSeq datasets of M. tuberculosis grown on glycerol and cholesterol. We show that TRANSIT can be used to discover genes which have been previously implicated for growth on these carbon sources. TRANSIT is written in Python, and thus can be run on Windows, OSX and Linux platforms. The source code is distributed under the GNU GPL v3 license and can be obtained from the following GitHub repository: https://github.com/mad-lab/transit. PMID:26447887

  12. TRANSIT - A Software Tool for Himar1 TnSeq Analysis

    PubMed Central

    DeJesus, Michael A.; Ambadipudi, Chaitra; Baker, Richard; Sassetti, Christopher; Ioerger, Thomas R.

    2015-01-01

    TnSeq has become a popular technique for determining the essentiality of genomic regions in bacterial organisms. Several methods have been developed to analyze the wealth of data that has been obtained through TnSeq experiments. We developed a tool for analyzing Himar1 TnSeq data called TRANSIT. TRANSIT provides a graphical interface to three different statistical methods for analyzing TnSeq data. These methods cover a variety of approaches capable of identifying essential genes in individual datasets as well as comparative analysis between conditions. We demonstrate the utility of this software by analyzing TnSeq datasets of M. tuberculosis grown on glycerol and cholesterol. We show that TRANSIT can be used to discover genes which have been previously implicated for growth on these carbon sources. TRANSIT is written in Python, and thus can be run on Windows, OSX and Linux platforms. The source code is distributed under the GNU GPL v3 license and can be obtained from the following GitHub repository: https://github.com/mad-lab/transit PMID:26447887

  13. Quantitative assessment of Tn antigen in breast tissue micro-arrays using CdSe aqueous quantum dots.

    PubMed

    Au, Giang H T; Mejias, Linette; Swami, Vanlila K; Brooks, Ari D; Shih, Wan Y; Shih, Wei-Heng

    2014-03-01

    In this study, we examined the use of CdSe aqueous quantum dots (AQDs) each conjugated to three streptavidin as a fluorescent label to image Tn antigen expression in various breast tissues via a sandwich staining procedure where the primary monoclonal anti-Tn antibody was bound to the Tn antigen on the tissue, a biotin-labeled secondary antibody was bound to the primary anti-Tn antibody, and finally the streptavidin-conjugated AQDs were bound to the biotin on the secondary antibody. We evaluated the AQD staining of Tn antigen on tissue microarrays consisting of 395 cores from 115 cases including three tumor cores and one normal-tissue core from each breast cancer case and three tumor cores from each benign case. The results indicated AQD-Tn staining was positive in more than 90% of the cells in the cancer cores but not the cells in the normal-tissue cores and the benign tumor cores. As a result, AQD-Tn staining exhibited 95% sensitivity and 90% specificity in differentiating breast cancer against normal breast tissues and benign breast conditions. These results were better than the 90% sensitivity and 80% specificity exhibited by the corresponding horse radish peroxidase (HRP) staining using the same antibodies on the same tissues and those of previous studies that used different fluorescent labels to image Tn antigen. In addition to sensitivity and specificity, the current AQD-Tn staining with a definitive threshold was quantitative. PMID:24411673

  14. 76 FR 53115 - Foreign-Trade Zone 77-Memphis, TN; Application for Temporary/Interim Manufacturing Authority...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-25

    ... Authority; Flextronics Logistics USA, Inc. (Cell Phone/Mobile Handset Kitting); Memphis, TN An application... 77 at the Flextronics Logistics USA, Inc. (Flextronics) facility, located in Memphis, Tennessee....

  15. 2014 County Commissioner Satisfaction Survey

    E-print Network

    2014 County Commissioner Satisfaction Survey: CSU Extension Services in Colorado Survey Results Summary Report April 2015 #12;Colorado State University Extension 2014 County Commissioner Survey Results Introduction: 2014 County Commissioner Survey The seventh annual County Commissioner Satisfaction Survey

  16. Balance : Lancaster County's tragedy

    E-print Network

    Gingrich, Valerie (Valerie J.)

    2007-01-01

    Lancaster County, Pennsylvania residents are proud of their agricultural heritage. They do not want to see their farmland disappear. But the County continues to be developed into residential subdivisions. This thesis ...

  17. Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome

    SciTech Connect

    Shoemaker, N.B.; Getty, C.; Gardner, J.F.; Salyers, A.A.

    1986-03-01

    The gene for resistance to erythromycin and clindamycin, which is carried on the conjugative Bacteroides plasmid, pBF4, has been shown previously to be part of an element (Tn4351) that transposes in Escherichia coli. The authors have now introduced Tn4351 into Bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range IncP plasmid R751 (R751::Tn4351) and (ii) pSS-2, a chimeric plasmid which contains 33 kilobases of pBF4 (including Tn4351) cloned into the IncQ plasmid RSF1010 and which is mobilized by R751. When E. coli HB101, carrying either R751::Tn4351 or R751 and pSS-2, was mated with B. uniformis under aerobic conditions, Em/sup r/ transconjugants were detected at a frequency of 10 /sup -6/ to 10/sup -5/ (R751::Tn4351) or 10/sup -8/ to 10/sup -6/ (R751 and pSS-2). In matings involving pSS-2, all Em/sup r/ transconjugants contained simple insertions of Tn4351 in the chromosome, whereas in matings involving R751::Tn4351, about half of the Em/sup r/ transconjugants had R751 cointegrated with Tn4351 in the chromosome. Of the Em/sup r/ transconjugants, 13% were auxotrophs. Bacteroides spp. which had R751 cointegrated with Tn4351 in the chromosome did not transfer R751 or Tn4351 to E. coli HB101 or to isogenic B. uniformis, nor did the integrated R751 mobilize pE5-2, an E. coli-Bacteroides shuttle vector that contains a transfer origin that is recognized by R751.

  18. Dona Ana County Experimental

    E-print Network

    Laughlin, Robert B.

    Reservoir Luna County Sierra County Otero County Lincoln National Forest 25 10 10 54 70 54 54 54 82 70 NM 1: Revisions to the National Landscape Conservation System included in Public Law 111-11 are not yet San Andres National Wildlife Refuge White Sands National Monument Caballo Reservoir Elephant Butte

  19. 77 FR 21505 - Proposed Amendment of Class D and E Airspace; Blountville, TN, and Revocation of Class E Airspace...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-10

    ... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a Regulatory Evaluation.... 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by reference in 14 CFR..., TN, and Revocation of Class E Airspace; Tri-City, TN AGENCY: Federal Aviation Administration...

  20. FATE OF TN5 MUTANTS OF ROOT GROWTH-INHIBITING PSEUDOMONAS SP. IN INTACT SOIL-CORE MICROCOSMS

    EPA Science Inventory

    Transposon Tn5 mutants of a wheat root growth-inhibiting nonfluorescent Pseudomonas sp. were inoculated into intact soil-core microcosms to determine the utility of intact soil cores for evaluating the fate and transport of microorganisms in agricultural ecosystems. ransposon Tn5...

  1. 76 FR 39379 - Expansion of Foreign-Trade Zone 78; Nashville, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... public comment has been given in the Federal Register (75 FR 69398, 11/12/10) and the application has... From the Federal Register Online via the Government Printing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Expansion of Foreign-Trade Zone 78; Nashville, TN Pursuant to its authority under...

  2. 75 FR 8414 - Tennessee Disaster # TN-00036 Declaration of Economic Injury

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-24

    ... Tennessee Disaster TN-00036 Declaration of Economic Injury AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Economic Injury Disaster Loan (EIDL) declaration for the... declaration, applications for economic injury disaster loans may be filed at the address listed above or...

  3. 75 FR 65584 - Proposed Amendment of Class E Airspace; Savannah, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-26

    ... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of..., 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by... action proposes to amend Class E Airspace at Savannah, TN, as the Pinhook Non-Directional Beacon...

  4. Insertion of Tn916 in Neisseria meningitidis resulting in loss of group B capsular polysaccharide.

    PubMed Central

    Stephens, D S; Swartley, J S; Kathariou, S; Morse, S A

    1991-01-01

    We recently found that the 16.4-kb conjugative transposon Tn916 could be introduced into Neisseria meningitidis by transformation and that it appeared to transpose to many different sites in the chromosome of recipient meningococci. In order to identify transposon-induced alterations of specific meningococcal virulence determinants, a library of meningococcal Tetr transformants containing Tn916 was made and screened for those altered in the production of group B capsular polysaccharide. A capsule-defective mutant, M7, was identified by using monoclonal and polyclonal antisera to group B polysaccharide in immunoblot and agar antiserum procedures. Growth of M7 was similar to that of the parent strain. M7 produced no group B capsular polysaccharide by rocket immunoelectrophoresis, and the mutation was stable during laboratory passage. The capsule-defective phenotype was linked to Tetr, as demonstrated by immunoblot and Southern blot analysis of progeny Tetr transformants (transformants of the parent strain obtained with DNA from M7). A capsule-deficient mutant, O8, was identified by using a similar approach. Analysis of the Tn916 insertions in M7 and O8 indicated that a significant portion of the transposon on either side of the tetM determinant had been lost. The ability of Tn916 to generate defined, stable mutations in meningococcal virulence determinants is demonstrated by our study. Images PMID:1657783

  5. 75 FR 28302 - American Food and Vending Spring Hill, TN; Notice of Negative Determination Regarding Application...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-20

    ...TA-W-72,606] American Food and Vending Spring Hill, TN; Notice of Negative Determination...workers at American Food and Vending, Spring Hill, Tennessee, was based on the findings...provided services to General Motors, Spring Hill, Tennessee, and were laid off...

  6. 77 FR 17360 - Proposed Amendment of Class E Airspace; Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of... continues to read as follows: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3... International Airport, TN (Lat. 35 02'33'' N., long. 89 58'36'' W.) ] Olive Branch, MS, Olive Branch...

  7. 77 FR 4007 - Grant of Authority for Subzone Status, Delta Faucet Company (Faucets), Jackson, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-26

    ... the Federal Register (75 FR 33765-33766, 6-15-2010) and the application has been processed pursuant to...-Trade Zones Board Grant of Authority for Subzone Status, Delta Faucet Company (Faucets), Jackson, TN... special-purpose subzone at the faucet manufacturing facility of Delta Faucet Company, in...

  8. Lactobacillus plantarum TN8 exhibits protective effects on lipid, hepatic and renal profiles in obese rat.

    PubMed

    Ben Salah, Riadh; Trabelsi, Imen; Hamden, Khaled; Chouayekh, Hichem; Bejar, Samir

    2013-10-01

    This study aimed to first investigate the immuno-modulatory effects of six newly isolated lactic acid bacteria (LAB) on the peripheral blood mononuclear cells (PBMC) of Wistar rats. Except for Lactobacillus plantarum TN8, all the other strains were noted to induce high levels of pro-inflammatory cytokine IL-12 and low levels of anti-inflammatory cytokine IL-10. The strains also generated low ratios of IL-10/IL-12 cytokine. Strain TN8 was, on the other hand, noted to induce an increase in anti-inflammatory IL-10 cytokine secretion rates and a decrease in pro-inflammatory IL-12, IFN-? and TNF-? cytokine production. The oral administration of TN8 improved the hepatic and urinary functions of obese rats by inducing decreases (P<0.05) in alanine amino transferase (ALAT), gamma glutamyl transferase (GGT), plasmatic triglycerides, total cholesterol concentrations, creatinine, urea, and body weight when compared to the control group of animals that underwent an increase in aspartate amino transferase (ASAT) and high density lipoprotein (HDL). Overall, the findings indicate that strain TN8 exhibited a number of attractive properties that might open new promising opportunities for the improvement of various parameters related to animal health performance and the avoidance of antibiotics and drugs as promoting factors. PMID:23891961

  9. 78 FR 27029 - Modification of Class C Airspace; Nashville International Airport; TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... International Airport, TN, Class C airspace area (78 FR 6257). Interested parties were invited to participate in... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a regulatory evaluation... FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The incorporation by reference in 14 CFR...

  10. 75 FR 28303 - Setco Automotive, Inc., Paris, TN; Notice of Revised Determination on Reconsideration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-20

    ...TA-W-71,401] Setco Automotive, Inc., Paris, TN; Notice of Revised Determination...that workers of Setco Automotive, Inc., Paris, Tennessee meet the worker group certification...All workers of Setco Automotive, Inc., Paris, Tennessee, who became totally or...

  11. 75 FR 52364 - Notice of Intent To Repatriate Cultural Items: Memphis Pink Palace Museum, Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF THE INTERIOR National Park Service Notice of Intent To Repatriate Cultural Items: Memphis Pink Palace Museum, Memphis, TN AGENCY: National Park Service, Interior. ACTION: Notice. Notice is here given in accordance with the Native American Graves Protection...

  12. 75 FR 28302 - American Food and Vending Spring Hill, TN; Notice of Negative Determination Regarding Application...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-20

    ... (janitorial, maintenance, and hazardous waste disposal) that were directly involved in the production process... and Training Administration American Food and Vending Spring Hill, TN; Notice of Negative... published in the Federal Register on April 23, 2010 (75 FR 21358). Pursuant to 29 CFR...

  13. 78 FR 9988 - Noise Exposure Map Notice Nashville Interntional Airport, Nashville, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-12

    ... Federal Aviation Administration Noise Exposure Map Notice Nashville Interntional Airport, Nashville, TN... Administration (FAA) announces its determination that the Noise Exposure Maps submitted by Metropolitan Nashville.... DATES: Effective Date: The effective date of the FAA's determination on the noise exposure maps...

  14. ION GPS '99, 14-17 September 1999, Nashville, TN Virtual Differential GPS & Road

    E-print Network

    Faulds, James E.

    ION GPS '99, 14-17 September 1999, Nashville, TN Virtual Differential GPS & Road Reduction company specializing in GPS and GIS R&D, and is also a lecturer in Geographical Information Science Fellowship to research GPS and digital mapping integra- tion for vehicle tracking and navigation. Professor

  15. Long-Term Variations of TN and TP in Four Typical Chinese Lakes

    NASA Astrophysics Data System (ADS)

    Huang, J.; Xu, Q.; Xi, B.; Wang, X.; Li, W.

    2014-12-01

    Few studies have examined long-term variations in the nutrients that cause lake eutrophication, namely total nitrogen (TN) and total phosphorous (TP). As a result, little is known about the peaks/troughs and trends in TN and TP in lakes known to be vulnerable to eutrophication such as Lake Dongting, Lake Poyang, Lake Chao, and Lake Tai, all of which are located in the Yangtze River basin of China. The objectives of this study were therefore to: 1) detect possible step changes in nutrient time series data; and 2) elucidate TN and TP temporal trends at multiple (annual, seasonal, and monthly) time scales, as influenced by factors such as river-lake connectivity and lake hydrologic conditions (water depth and inflow source). The distribution-free cumulative sum technique and modified Mann-Kendall approach were applied to the long-term nutrient data measured for these four typical lakes and revealed that both TN and TP in Lake Dongting and Lake Poyang exhibited an upward trend at annual, seasonal, and monthly time scales, although they are oligotrophic lakes. The nutrient concentrations in Lake Dongting underwent a step change around 2003, which can be attributed to point and non-point source pollution, overexploitation, and dam construction. The TN values for Lake Chao and Lake Tai experienced step changes in 1999 and 2002, respectively. Before the step-change years, a significant (p-value < 0.05) upward trend in both TN and TP was detected for Lake Chao at the annual scale as well as in summer and May, but for Lake Tai this was manifested at the annual scale only. In contrast, after the step-change years, a significant downward trend was detected for both lakes at the corresponding time scales. As expected, the TN and TP concentrations in Lake Chao and Lake Tai, which are located in the lower basin of the Yangtze River, were noticeably higher than those in Lake Dongting and Lake Poyang, which are located in the middle basin.

  16. 40 CFR 81.343 - Tennessee.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Editorial Note: For Federal Register citations affecting 81.343, see the List of CFR Sections Affected... Robertson County X Rutherford County X Scott County X Sequatchie County X Sevier County X Shelby County X... Putnam County X Rhea County X Roane County X Robertson County X Rutherford County X Scott County...

  17. 40 CFR 81.343 - Tennessee.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... affecting 81.343, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... Robertson County X Rutherford County X Scott County X Sequatchie County X Sevier County X Shelby County X... Putnam County X Rhea County X Roane County X Robertson County X Rutherford County X Scott County...

  18. 40 CFR 81.343 - Tennessee.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... Editorial Note: For Federal Register citations affecting 81.343, see the List of CFR Sections Affected... Robertson County X Rutherford County X Scott County X Sequatchie County X Sevier County X Shelby County X... Putnam County X Rhea County X Roane County X Robertson County X Rutherford County X Scott County...

  19. 5. Photocopy of May 21, 1936 photograph showing the interior ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Photocopy of May 21, 1936 photograph showing the interior of the Big House (main portion, living-bedroom). The woman is Hettie Walker. The fireplace is on the house's front side. The original photograph was taken by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8086. - Walker Family Farm, Big House, Gatlinburg, Sevier County, TN

  20. 4. Photocopy of photograph showing the corn crib as it ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Photocopy of photograph showing the corn crib as it looked on May 21, 1936. This structure was also used as a gear shed. Immediately to the right of the shed is Hettie Walker and an unidentified family member or friend. The original photograph was taken by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8091. - Walker Family Farm, Corn Crib, Gatlinburg, Sevier County, TN

  1. Tn-Seq Explorer: A Tool for Analysis of High-Throughput Sequencing Data of Transposon Mutant Libraries

    PubMed Central

    Solaimanpour, Sina; Sarmiento, Felipe; Mrzek, Jan

    2015-01-01

    Tn-seq is a high throughput technique for analysis of transposon mutant libraries. Tn-seq Explorer was developed as a convenient and easy-to-use package of tools for exploration of the Tn-seq data. In a typical application, the user will have obtained a collection of sequence reads adjacent to transposon insertions in a reference genome. The reads are first aligned to the reference genome using one of the tools available for this task. Tn-seq Explorer reads the alignment and the gene annotation, and provides the user with a set of tools to investigate the data and identify possibly essential or advantageous genes as those that contain significantly low counts of transposon insertions. Emphasis is placed on providing flexibility in selecting parameters and methodology most appropriate for each particular dataset. Tn-seq Explorer is written in Java as a menu-driven, stand-alone application. It was tested on Windows, Mac OS, and Linux operating systems. The source code is distributed under the terms of GNU General Public License. The program and the source code are available for download at http://www.cmbl.uga.edu/downloads/programs/Tn_seq_Explorer/ and https://github.com/sina-cb/Tn-seqExplorer. PMID:25938432

  2. Tn-seq explorer: a tool for analysis of high-throughput sequencing data of transposon mutant libraries.

    PubMed

    Solaimanpour, Sina; Sarmiento, Felipe; Mrzek, Jan

    2015-01-01

    Tn-seq is a high throughput technique for analysis of transposon mutant libraries. Tn-seq Explorer was developed as a convenient and easy-to-use package of tools for exploration of the Tn-seq data. In a typical application, the user will have obtained a collection of sequence reads adjacent to transposon insertions in a reference genome. The reads are first aligned to the reference genome using one of the tools available for this task. Tn-seq Explorer reads the alignment and the gene annotation, and provides the user with a set of tools to investigate the data and identify possibly essential or advantageous genes as those that contain significantly low counts of transposon insertions. Emphasis is placed on providing flexibility in selecting parameters and methodology most appropriate for each particular dataset. Tn-seq Explorer is written in Java as a menu-driven, stand-alone application. It was tested on Windows, Mac OS, and Linux operating systems. The source code is distributed under the terms of GNU General Public License. The program and the source code are available for download at http://www.cmbl.uga.edu/downloads/programs/Tn_seq_Explorer/ and https://github.com/sina-cb/Tn-seqExplorer. PMID:25938432

  3. [Effects of Total Nitrogen and BOD5/TN on Anaerobic Ammonium Oxidation-Denitrification Synergistic Interaction of Mature Landfill Leachate in Aged Refuse Bioreactor].

    PubMed

    Yang, Ying-ying; Chen, Yi; Lj, Ming-jie; Xie, Bing

    2015-04-01

    Mature landfill leachate, featured with high ammonium (NH4+) content and low biodegrade ability (low BOD5/COD ratio), is hard to be treated. This study mainly focused on the effects of influent TN (total nitrogen) loading and BOD5/TN ratios on the nitrogen removal efficiency of landfill leachate by landfill bioreactors. The results showed that when the influent total nitrogen loading was in the range of 15 g x (m3 x d)(-1) to 25 g x (m3 x d)(-1), the TN removal loading could remain stable between 10 g x (m3 x d)(-1) and 12 g x (m3 x d)(-1), while the TN removal efficiency decreased from 67.7% to 60.2% with the increasing loading. Therefore, TN loading shocks would lower the bioreactor's TN removal rate, but would not affect its TN removal loading. When the influent BOD5/TN ratio was increased from 0.3 to 0.4 and the TN loading was controlled at 9 g x (m3 x d)(-1), the TN removal rates were increased from 79.9% to 89.9% and 86.2% in anaerobic and aerobic, respectively. This implied that properly enhancing BOD5/TN ratio could significantly increase the TN removal efficiency of the bioreactor, and the effect was more significant under anaerobic condition. Analysis of nitrogen removal pathways showed that denitrification and anammox could take place synergistically in landfill bioreactor. PMID:26164920

  4. A Tn7-like transposon is present in the glmUS region of the obligately chemoautolithotrophic bacterium Thiobacillus ferrooxidans.

    PubMed

    Oppon, J C; Sarnovsky, R J; Craig, N L; Rawlings, D E

    1998-06-01

    The region downstream of the Thiobacillus ferrooxidans ATCC 33020 atp operon was examined, and the genes encoding N-acetylglucosamine-1-uridyltransferase (glmU) and glucosamine synthetase (glmS) were found. This atpEFHAGDC-glmUS gene order is identical to that of Escherichia coli. The T. ferrooxidans glmS gene was shown to complement E. coli glmS mutants for growth on minimal medium lacking glucosamine. A Tn7-like transposon, Tn5468, was found inserted into the region immediately downstream of the glmS gene in a manner similar to the site-specific insertion of transposon Tn7 within the termination region of the E. coli glmS gene. Tn5468 was sequenced, and Tn7-like terminal repeat sequences as well as several open reading frames which are related to the Tn7 transposition genes tnsA, tnsB, tnsC, and tnsD were found. Tn5468 is the closest relative of Tn7 to have been characterized to date. Southern blot hybridization indicated that a similar or identical transposon was present in three T. ferrooxidans strains isolated from different parts of the world but not in two Thiobacillus thiooxidans strains or a Leptospirillum ferrooxidans strain. Since T. ferrooxidans is an obligately acidophilic autotroph and E. coli is a heterotroph, ancestors of the Tn7-like transposons must have been active in a variety of physiologically different bacteria so that their descendants are now found in bacteria that occupy very different ecological niches. PMID:9603897

  5. Genechip-detecting mutations in exon 8 in cTnI gene associated with FHCM

    NASA Astrophysics Data System (ADS)

    Zhang, Yuanying; He, Nongyue; Guo, Huishi; Yang, Di; Wan, Wenhui; Bian, Zhiping; Zhang, Jinan

    2005-01-01

    As the rate of gene discovery accelerates, more efficient methods are needed to analyze genes in human tissues. Genechip, a kind of new device, is composed of DNA probes immobilized on a solid substrate. With the advantage of the high throughput information, genechip has become one of the best solutions to detect and analyse the mutations in genes. Hypertrophic cardiomyopathy (HCM), the most common cause of the sudden death in the young, is one of the diseases damaging people health most badly. It is an autosomal dominant disease. More than 55% of the HCM patients are genetic. The mutations of exon 8 in the Cardiac troponin I (cTnI) gene are closely associated with Family Hypertrophic Cardiomyopathy (FHCM). Our purpose is to perform the assay of the mutations in exon 8 in cTnI gene based on the genechip theory and technology. Special probes were designed to fabricate the genechip to detect the mutations in cTnI gene simultaneously. We designed two oligonucleotide sequences 5"-end labeled with fluorescein, one simulating wild-type and the other simulating mutant. We mixed oligonucleotide I and II together to simulate heterozygote. After optimizing the hybridization protocols, the fabricated genechip can detect the mutations in exon 8 in cTnI gene with relative high sensitivity and specificity. When applying the fabricated genechip to detect the target DNA sequence, we found that the fully complementary probe gave a fluorescent signal almost 50% stronger than that of the one base mismatched one, which is in accordance with the result from theoretic estimate. It is believed that an applicable special genechip can be developed for investigating and diagnosing FHCM after further improvement.

  6. Design analysis report for the TN-WHC cask and transportation system

    SciTech Connect

    Brisbin, S.A., Fluor Daniel Hanford

    1997-02-13

    This document presents the evaluation of the Spent Nuclear Fuel Cask and Transportation System. The system design was developed by Transnuclear, Inc. and its team members NAC International, Nelson Manufacturing, Precision Components Corporation, and Numatec, Inc. The cask is designated the TN-WHC cask. This report describes the design features and presents preliminary analyses performed to size critical dimensions of the system while meeting the requirements of the performance specification.

  7. ROLE OF NA+ IN TRANSPORT OF HG2+ AND INDUCTION OF THE TN21 "MER" OPERON

    EPA Science Inventory

    The effects of sodium ions on the uptake of Hg 2 + and induction of the TN21 mer operon were studied using E. coli HMS174 harboring the reporter plasmids pRB28 and pOS14. lasmid pRB28 carries merRT' and pOS14 carries merRTPC of the mer operon, both cloned upstream of a promoterle...

  8. Characterization of Tn917 insertion mutants of Staphylococcus epidermidis affected in biofilm formation.

    PubMed Central

    Heilmann, C; Gerke, C; Perdreau-Remington, F; Gtz, F

    1996-01-01

    Biofilm formation is thought to result from the concerted action of primary attachment to a specific surface and accumulation in multilayered cell clusters. Here we describe the isolation and characterization of transposon (Tn917) mutants of Staphylococcus epidermidis O-47 which were biofilm negative in the polystyrene microtiter plate assay. Among 5,000 Tn917 insertion mutants, 4 biofilm-negative mutants were isolated. Each mutant carried one copy of Tn917. The mutants were divided into two phenotypic classes: class A (mut1 and mut1a) and class B (mut2 and mut2a). Mutants of phenotypic class A lacked four cell surface proteins, were less hydrophobic, and were affected in primary attachment to polystyrene, but were still able to form multilayered cell clusters. They were able to form a biofilm on a glass surface, a trait that was even more pronounced than in the wild-type stain O-47. Loss of several surface proteins might have led to the reduced surface hydrophilic structures, thus favoring primary attachment to a glass surface and leading to subsequent biofilm formation. Mutants of phenotype class B were able to attach to polystyrene but were unable to form multilayered cell clusters, had unchanged cell surface proteins and hydrophobicity, and were unable to form a biofilm on a glass surface, mut1 and mut2 could be complemented by wild-type DNA fragments containing the Tn917 insertion sites of mut1 and mut2, respectively. The complemented biofilm-positive clone mut1 (pRC20) produced a 60-kDa protein which is postulated to function as the adhesin for binding to plastic. The traits of binding to polystyrene and the ability to form multilayered cell clusters are phenotypically and genetically distinct. PMID:8557351

  9. Silicon nanowire biosensors for detection of cardiac troponin I (cTnI) with high sensitivity.

    PubMed

    Kim, Kihyun; Park, Chanoh; Kwon, Donghoon; Kim, Donghoon; Meyyappan, M; Jeon, Sangmin; Lee, Jeong-Soo

    2016-03-15

    We have demonstrated highly sensitive and label-free detection of cardiac troponin I (cTnI), a biomarker for diagnosis of acute myocardial infarction, using silicon nanowire field-effect transistors. A honeycomb-like structure is utilized for nanowire configuration to offer improved electrical performance and increased sensing area. The fabricated devices show n-type behavior with a relatively high ON-OFF current ratio, small sub-threshold swing and low gate leakage current. Monoclonal antibodies for cTnI were covalently immobilized on the nanowire surface and the attachment of antibodies is clearly visualized by atomic force microscope. The sensitivity with various concentrations of buffer solution was also investigated in order to determine the optimal buffer condition. The devices exhibit highest sensitivity under buffer solutions with low ion concentration. In addition, the detection limit of the sensor is as low as ?5pg/mL, the lowest reported in the literature to date and nearly an order of magnitude smaller than the suggested threshold limit. The fabricated devices demonstrate a good selectivity for detecting cTnI. PMID:26496224

  10. Retrieving TN and TP Concentration of Urban River From High Resolution IKONOS Multispectral Imagery

    NASA Astrophysics Data System (ADS)

    Zhang, Y.; Liu, J.; Zhang, L.; Song, X.

    2014-12-01

    Total nitrogen (TN) and Total phosphorus (TP) are widely known as two important indexes to measure China urban rivers, and the technique of remote sensing plays an important role in quantitatively monitoring the dynamic change and timely grasping the status of urban rivers. Taking Wen-rui Tang River as examples, this paper develops both multiple regressions (MR) model and artificial neural networks (ANN) model to estimate TN and TP concentration from high resolution IKONOS image data and in situ water samples collected concurrently with satellite overpass. By analyzing determination coefficients (R2) and relative root mean square error (RMSE), it is found that the measured and estimated values of both MR and ANN models are in good agreement (R2>0.85 and RMSE<2.50), and the estimated accuracy using ANN model is better (R2>0.86 and RMSE<0.89). The results also present the potential of high resolution IKONOS multispectral imagery to apply to urban rivers. The spatial distribution maps of TP and TN concentration generated by ANN model present apparent spatial variations and inform the decision makers of water quality variations in Wen-rui Tang River. The approach developed in this study proves to be effective and has the potential to be applied over urban rivers for water quality monitoring.

  11. 75 FR 52367 - Notice of Inventory Completion: Memphis Pink Palace Museum, Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ...removed from the Togo site (3CS24), Cross County, AR, during amateur excavations. The human remains were donated to the museum...near the Walls site (22DS500), DeSoto County, MS, during amateur excavations. The human remains were donated to the...

  12. Comprehensive identification of virulence factors required for respiratory melioidosis using Tn-seq mutagenesis

    PubMed Central

    Gutierrez, Maria G.; Yoder-Himes, Deborah R.; Warawa, Jonathan M.

    2015-01-01

    Respiratory melioidosis is a disease presentation of the biodefense pathogen, Burkholderia pseudomallei, which is frequently associated with a lethal septicemic spread of the bacteria. We have recently developed an improved respiratory melioidosis model to study the pathogenesis of Burkholderia pseudomallei in the lung (intubation-mediated intratracheal [IMIT] inoculation), which more closely models descriptions of human melioidosis, including prominent septicemic spread from the lung and reduced involvement of the upper respiratory tract. We previously demonstrated that the Type 3 Secretion System cluster 3 (T3SS3) is a critical virulence determinant for B. pseudomallei when delivered directly into the lung. We decided to comprehensively identify all virulence determinants required for respiratory melioidosis using the Tn-seq phenotypic screen, as well as to investigate which virulence determinants are required for dissemination to the liver and spleen. While previous studies have used Tn-seq to identify essential genes for in vitro cultured B. pseudomallei, this represents the first study to use Tn-seq to identify genes required for in vivo fitness. Consistent with our previous findings, we identified T3SS3 as the largest genetic cluster required for fitness in the lung. Furthermore, we identified capsular polysaccharide and Type 6 Secretion System cluster 5 (T6SS5) as the two additional major genetic clusters facilitating respiratory melioidosis. Importantly, Tn-seq did not identify additional, novel large genetic systems supporting respiratory melioidosis, although these studies identified additional small gene clusters that may also play crucial roles in lung fitness. Interestingly, other previously identified virulence determinants do not appear to be required for lung fitness, such as lipopolysaccharide. The role of T3SS3, capsule, and T6SS5 in lung fitness was validated by competition studies, but only T3SS3 was found to be important for respiratory melioidosis when delivered as a single strain challenge, suggesting that competition studies may provide a higher resolution analysis of fitness factors in the lung. The use of Tn-seq phenotypic screening also provided key insights into the selective pressure encountered in the liver. PMID:26583079

  13. 75 FR 61696 - Foreign-Trade Zone 148-Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ...Subzone; Toho Tenax America, Inc. (Carbon Fiber and Oxidized Polyacrylonitrile Fiber Manufacturing); Rockwood, TN An application...special-purpose subzone status for the carbon fiber and oxidized polyacrylonitrile fiber (OPF)...

  14. 77 FR 73978 - Foreign-Trade Zone 148-Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-12

    ...Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing Authority), Opening of Comment...Tennessee, with authority to manufacture carbon fiber for export and oxidized polyacrylonitrile fiber (Board Order 1868, 77 FR 69435,...

  15. *Corresponding author. E-mail address: caspar@qt.tn.tudelft.nl (C.H. van der Wal)

    E-print Network

    van der Wal, Caspar H.

    *Corresponding author. E-mail address: caspar@qt.tn.tudelft.nl (C.H. van der Wal) Physica B 280 (2000) 243}244 Quantum transitions of a small Josephson junction array Caspar H. van der Wal*, P. Kuiper

  16. 77 FR 68818 - Notice of Inventory Completion: U.S. Department of Energy Oak Ridge Office, Oak Ridge, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-16

    ...of Inventory Completion: U.S. Department of Energy Oak Ridge Office, Oak Ridge, TN AGENCY: National Park Service, Interior...SUMMARY: The U.S. Department of Energy Oak Ridge Office has completed an inventory of human...

  17. 78 FR 2431 - Notice of Inventory Completion: U.S. Department of Energy Oak Ridge Office, Oak Ridge, TN...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-11

    ...of Inventory Completion: U.S. Department of Energy Oak Ridge Office, Oak Ridge, TN; Correction AGENCY: National Park Service...SUMMARY: The U.S. Department of Energy Oak Ridge Office has corrected an inventory of human...

  18. 76 FR 60802 - Foreign-Trade Zone 77-Memphis, TN; Withdrawal of Request for Temporary/Interim Manufacturing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-30

    ...Withdrawal of Request for Temporary/Interim Manufacturing Authority; Flextronics Logistics USA, Inc., (Cell Phone/Mobile Handset Kitting). Memphis, TN Notice is hereby given of the withdrawal of the application of the City of...

  19. Ca(2+)-regulatory function of the inhibitory peptide region of cardiac troponin I is aided by the C-terminus of cardiac troponin T: Effects of familial hypertrophic cardiomyopathy mutations cTnI R145G and cTnT R278C, alone and in combination, on filament sliding.

    PubMed

    Brunet, Nicolas M; Chase, P Bryant; Mihajlovi?, Goran; Schoffstall, Brenda

    2014-06-15

    Investigations of cardiomyopathy mutations in Ca(2+) regulatory proteins troponin and tropomyosin provide crucial information about cardiac disease mechanisms, and also provide insights into functional domains in the affected polypeptides. Hypertrophic cardiomyopathy-associated mutations TnI R145G, located within the inhibitory peptide (Ip) of human cardiac troponin I (hcTnI), and TnT R278C, located immediately C-terminal to the IT arm in human cardiac troponin T (hcTnT), share some remarkable features: structurally, biochemically, and pathologically. Using bioinformatics, we find compelling evidence that TnI and TnT, and more specifically the affected regions of hcTnI and hcTnT, may be related not just structurally but also evolutionarily. To test for functional interactions of these mutations on Ca(2+)-regulation, we generated and characterized Tn complexes containing either mutation alone, or both mutations simultaneously. The most important results from in vitro motility assays (varying [Ca(2+)], temperature or HMM density) show that the TnT mutant "rescued" some deleterious effects of the TnI mutant at high Ca(2+), but exacerbated the loss of function, i.e., switching off the actomyosin interaction, at low Ca(2+). Taken together, our experimental results suggest that the C-terminus of cTnT aids Ca(2+)-regulatory function of cTnI Ip within the troponin complex. PMID:24418317

  20. San Diego County Reservation

    E-print Network

    Laughlin, Robert B.

    Corps Base Yuma Marine CorpsAir Station Yuma Proving Ground Havasu National Wildlife Refuge Imperial National Wildlife Refuge Kofa National Wildlife Refuge Cibola National Wildlife Refuge Bill Williams River National Wildlife Refuge Sonny Bono Salton Sea National Wildlife Refuge Yuma County Imperial County Mohave

  1. An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.

    PubMed

    Glassing, Angela; Lewis, Thomas A

    2015-11-01

    An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio. PMID:26341612

  2. nh Gi Tn Hi Ti Nguyn Thin Nhin do Trn Du Deep Horizon Khi phc b bin sng v cc

    E-print Network

    nh Gi Tn Hi Ti Nguyn Thin Nhin do Trn Du Deep Horizon Khi phc b bin sng v cc rng san h rng san h ti ca sng Mississippi c xut s phc hi cc rng san h ngp triu v cn triu, s dng k thut b sng nh, qua hn ch xi mn b bin v tn hi n khu m ly. Qua thi gian, cc chn sng, khu vc san h

  3. 76 FR 12306 - Revisions to the California State Implementation Plan, Imperial County, Kern County, and Ventura...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ...County, and Ventura County; Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District...

  4. 76 FR 12280 - Revisions to the California State Implementation Plan, for Imperial County, Kern County, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ...County, and Ventura County; Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District...

  5. Nitrogen removal from low COD/TN ratio manure-free piggery wastewater within an upflow microaerobic sludge reactor.

    PubMed

    Meng, Jia; Li, Jiuling; Li, Jianzheng; Antwi, Philip; Deng, Kaiwen; Wang, Cheng; Buelna, Gerardo

    2015-12-01

    An upflow microaerobic sludge reactor (UMSR) was constructed in treating manure-free piggery wastewater with high ammonium concentration and a COD/TN ratio as low as 0.84. The UMSR offered an outstanding removal of NH4(+)-N and TN at 35C and hydraulic retention time 8h subsequent to inoculated sludge acclimation. A short NO2(-)-N accumulation phase was observed whenever there was a considerable increase in TN loading rate (NLR), but decreased rapidly along with an evident increase in TN removal. Fed with raw wastewater at a NLR of 1.10kg/(m(3)d), the average COD, NH4(+)-N and TN removal reached 0.72, 0.76 and 0.94kg/(m(3)d), respectively. Inference drawn from stoichiometry based on the potential nitrogen removal pathways and the C/N ratio required by denitrification indicated that anammox was the main mechanism for NH4(+)-N and TN removal in the UMSR. PMID:26409858

  6. A new murine IgG1 anti-Tn monoclonal antibody with in vivo anti-tumor activity.

    PubMed

    Welinder, Charlotte; Baldetorp, Bo; Borrebaeck, Carl; Fredlund, Britt-Marie; Jansson, Bo

    2011-08-01

    The Tn antigen (GalNAc ?-O-Ser/Thr) is heterogeneously synthesized by a variety of tumors and contains an epitope defined by lectins and antibodies as a cluster of ?GalNAc carbohydrates synthesized within a peptide sequence, which is rich in serine and/or threonine. The Tn antigen has been utilized as a target in vaccine experiments and also used as a biomarker for prognosis of different cancer forms. In this paper, we present a new monoclonal antibody, GOD3-2C4, with the clear hallmarks of an anti-Tn antibody. It was generated through somatic cell hybridization after immunization of a mouse with a tumor cell line and a Tn carrying mucin. The antibody recognizes synthetic Tn antigen and binds breast, colon, lung, ovarian and pancreas cancer. The GOD3-2C4 antibody has antibody-dependent cellular cytotoxicity activity against Jurkat cells in vitro, and for the first time, it can be shown that an anti-Tn antibody has a significant in vivo effect on a human cancer cell line grown as a xenograft in severe combined immunodeficiency mice. PMID:21470982

  7. Phage ?C2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains

    PubMed Central

    Goh, Shan; Hussain, Haitham; Chang, Barbara J.; Emmett, Warren; Riley, Thomas V.; Mullany, Peter

    2013-01-01

    ABSTRACT In this work, we show that Clostridiumdifficile phage ?C2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10?6 per PFU. The transductants were lysogenic for ?C2 and contained the erm(B) gene in a novel transposon, Tn6215. This element is 13,008bp in length and contains 17 putative open reading frames (ORFs). It could also be transferred at a lower frequency by filter mating. PMID:24255122

  8. Metabolite Profiling Reveals Abiotic Stress Tolerance in Tn5 Mutant of Pseudomonas putida

    PubMed Central

    Chaudhry, Vasvi; Bhatia, Anil; Bharti, Santosh Kumar; Mishra, Shashank Kumar; Chauhan, Puneet Singh; Mishra, Aradhana; Sidhu, Om Prakash; Nautiyal, Chandra Shekhar

    2015-01-01

    Pseudomonas is an efficient plant growthpromoting rhizobacteria (PGPR); however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5) mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC) and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE) and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS) pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA) on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic stress tolerant metabolites from the NBRI1108T suggest that Tn5 mutagenesis enhanced tolerance towards high temperature and drought. Tolerance to drought was further confirmed in greenhouse experiments with maize as host plant, where NBRI1108T showed relatively high biomass under drought conditions. PMID:25629312

  9. Enhanced self-association of mucins possessing the T and Tn carbohydrate cancer antigens at the single-molecule level

    PubMed Central

    Haugstad, Kristin E.; Gerken, Thomas A.; Stokke, Bjrn T.; Dam, Tarun K.; Brewer, C. Fred; Sletmoen, Marit

    2012-01-01

    Mucins are linear O-glycosylated glycoproteins involved in inflammation, cell adhesion and tumorigenesis. Cancer associated mucins often possess increased expression of the T (Gal?1,3GalNAc?Thr/Ser) and Tn (GalNAc?Thr/Ser) cancer antigens, which are diagnotic markers for several cancers including colon cancer. We have used AFM based single-molecule forced unbinding under near physiological conditions to investigate the self-interactions between porcine submaxillary mucin (PSM) as well as between PSM analogs possessing various carbohydrates including the T- and Tn-antigen. Distributions of unbinding forces and corresponding force loading rates were determined for force loading rates from 0.18 nN/s to 39 nN/s, and processed to yield most probable unbinding forces f* and lifetimes of the interactions. Parameter f* varied in the range 27 to 50 pN at force loading rates of about 2 nN/s among the various mucins. All mucin samples investigated showed self-interaction, but the tendency was greatest for PSM displaying only the Tn-antigen (Tn-PSM) or a mixture of Tn-, T-antigen and the trisaccharide Fuc?1,2Gal?1,3GalNAc (Tri-PSM). Weaker self-interactions were observed for native PSM (Fd-PSM), which consists of a nearly equal mixture of the longer core 1 blood group A tetrasaccharide (GalNAc?1,3(Fuc?1,2)Gal?1,3GalNAc?Ser/Thr) and Tn-antigen. The data are consistent with the truncated Tn and T glycans enhancing self-interaction of the mucins. These carbohydrate cancer antigens may thus play an active role in the disease by constitutively activating mucin and mucin-type receptors by self-association on cells. PMID:22428527

  10. The Sugar Creek zinc deposit, Jackson Co. TN -- Exploration history, geology and mineralization

    SciTech Connect

    Reinbold, G.; Moran, A.V.; Stevens, D.L. )

    1993-03-01

    During the 60's and 70's zinc exploration of central TN and KY was active. The Sugar Creek Project was one of several investigated by Exxon. The discovery hole, Cu 15, was drilled in early 1973. The Sugar Creek Zinc Deposit was acquired by Independence Mining Co. in 1986 and I.M.C. has subsequently completed additional drilling, both stepout and confirmation holes. A total of 137 holes for 300,833 ft have been drilled. The Sugar Creek deposit is a typical Tennessee zinc deposit (Mississippi Valley Type) which occurs in solution collapse breccias in the Lower Ordovician, Knox Dolomite. The Knox consists of fine grained dolomite with interlayered limestones and crystalline dolomite. Only scattered residual limestone is found in the Sugar Creek area. Collapse breccias have formed which control zinc deposition and are similar to other TN Zn. deposits. At Sugar Creek the types of breccias include: a vertically exaggerated glory hole breakthrough breccia which extends to within 137 ft. of the Knox unconformity, has 500 ft. of zinc mineralization with 8 significant zinc intervals; holes with stacked zinc intervals interpreted to be sides of breakthrough breccia; and single zinc intervals in laterally positioned bedded mineral zones. A total of 99 holes were drilled in the more intense mineralized areas. The ratio of ore to non ore holes is nearly 1 to 1. The mineralization is typical M.V.T. with predominantly sphalerite and only minor occurrences of galena, fluorite, pyrite, etc.

  11. Modeling in-situ uranium(VI) bioreduction at Oak Ridge, TN

    NASA Astrophysics Data System (ADS)

    Luo, J.; Weber, F.; Cirpka, O. A.; Wu, W.; Nyman, J. L.; Carley, J. M.; Jardine, P. M.; Criddle, C. S.; Kitanidis, P. K.

    2005-12-01

    A travel-time based reactive-transport model is developed to simulate an in-situ bioremediation experiment for demonstrating enhanced bioreduction of uranium(VI) at Oak Ridge, TN. The space to travel-time transformation is obtained from a conservative-tracer test. Reactive transport is solved numerically in time and travel-time coordinates. Reaction kinetics include aquatic equilibrium geochemistry, uranium sorption and precipitation, and the microbial reduction of nitrate, sulfate and U(VI). U(VI) sorption/desorption is described by a surface-complexation model and the characteristics of kinetic sorption/desorption is described by mass transfer between stagnant micro-pores and mobile flow zones. The model characterizes the succession of terminal electron accepting processes and the growth and decay of sulfate-reducing bacteria, concurrent with the enzymatic reduction of aqueous U(VI) species. The effective U(VI) reduction rate and sorption site distributions are determined by fitting the model simulation to an in-situ experiment at Oak Ridge, TN. Results indicate that (1) nitrate inhibits U(VI) reduction at the site; (2) the effective reduction rate of U(VI) is much smaller than the values reported for laboratory experiments; (3) U(VI) sorption/desorption is kinetically controlled; (4) pH and bicarbonate concentration varies over the experimental period, significantly influencing the U(VI) sorption/desorption; and (5) calcium concentration influences the concentrations of biodegradable U(VI) species.

  12. Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha

    SciTech Connect

    Chandra, T.S.; Friedrich, C.G.

    1986-05-01

    Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the inability to grow with reduced sulfur compounds (Sox/sup -/). Four classes of Sox/sup -/ mutants were obtained. Three were of different pleiotropic phenotypes: (i) unable to grow with formate, nitrate, and xanthine; (this class strongly suggested the involvement of a molybdenum cofactor in inorganic sulfur-oxidizing ability); (ii) no growth with hydrogen; (iii) slight growth with hydrogen and formate. Two plasmids, pHG41 (about 450 kilobase pairs) and pHG42 (110 kilobases), were identified in lysates of T. pantotropha. In one Sox/sup -/ mutant pHG41 could not be detected. Revertant analysis suggested that pHG41 and pHG42 were not involved in the Sox character.

  13. Single-site chromosomal Tn5 insertions affect the export of pectolytic and cellulolytic enzymes in Erwinia chrysanthemi EC16.

    PubMed Central

    Thurn, K K; Chatterjee, A K

    1985-01-01

    Exponentially growing cells of Erwinia chrysanthemi EC16 usually export about 98% of their pectate lyase (PL) and protease, about 40% of their polygalacturonase (PG), and about 60% of their cellulase (endoglucanase or carboxymethyl cellulase; CL). By using the R plasmid, pJB4JI (pPH1JI::Mu::Tn5), three independent Tn5 insertion mutants were obtained that exported normal levels of protease but 10% or less of PL, PG, and CL. Physical analysis revealed that single copies of Tn5 had inserted into the E. chrysanthemi chromosome, producing a similar export-defective (Out-) phenotype. The synthesis of PL, PG, and CL was not affected by the Tn5 insertions. These enzymes were released from the mutants on spheroplast formation, indicating that they were located in the periplasmic space. Tn5 insertions caused the loss of a 35-kilodalton periplasmic protein, but did not alter the outer membrane protein composition. The findings are discussed with respect to the current knowledge on protein export in gram-negative bacteria. Images PMID:3002271

  14. One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI.

    PubMed

    Tuteja, Satish K; Kukkar, Manil; Suri, C R; Paul, A K; Deep, Akash

    2015-04-15

    2-Aminobenzyl amine (2-ABA) functionalized graphene is proposed for the ultrasensitive immunosensing of Cardiac Troponin I (cTnI). 2-ABA was electrochemically polymerized on the graphene decorated interdigitated electrode to obtain the amine functionalized graphene (f-GN). The f-GN electrode was then modified with monoclonal anti-cTnI antibodies via Schiff reaction based chemistry. Detailed characteristics of the processes involved and the finally developed antibody conjugated f-GN interdigitated electrode have been studied. The above micro-device was used in a drain source configuration for the sensing of cTnI. A wide dynamic linear range of antigen detection (0.01-1ng/mL) is achieved with the limit of detection of 0.01ng/mL. The utility of the proposed sensing technique is demonstrated by successfully testing the antigen concentration in spiked serum samples. PMID:25460893

  15. Vegetation Monitoring in Orange County

    E-print Network

    Rose, Michael R.

    Vegetation Monitoring in Orange County Douglas Deutschman Professor of Biology Ecological Research and Restora1on in Orange County Jan 27, 2012 #12;Outline Monitoring in an Adap1ve Management Framework Vegeta1on Monitoring in Orange

  16. Snohomish County Biodiesel Project

    SciTech Connect

    Terrill Chang; Deanna Carveth

    2010-02-01

    Snohomish County in western Washington State began converting its vehicle fleet to use a blend of biodiesel and petroleum diesel in 2005. As prices for biodiesel rose due to increased demand for this cleaner-burning fuel, Snohomish County looked to its farmers to ???¢????????grow???¢??????? this fuel locally. Suitable seed crops that can be crushed to extract oil for use as biodiesel feedstock include canola, mustard, and camelina. The residue, or mash, has high value as an animal feed. County farmers began with 52 acres of canola and mustard crops in 2006, increasing to 250 acres and 356 tons in 2008. In 2009, this number decreased to about 150 acres and 300 tons due to increased price for mustard seed.

  17. Analysis of Tn antigenicity with a panel of new IgM and IgG1 monoclonal antibodies raised against leukemic cells.

    PubMed

    Blixt, Ola; Lavrova, Olga I; Mazurov, Dmitriy V; Cl, Emiliano; Kracun, Stjepan K; Bovin, Nicolai V; Filatov, Alexander V

    2012-04-01

    CD175 or Tn antigen is a carbohydrate moiety of N-acetylgalactosamine (GalNAc)?1-O- linked to the residue of amino acid serine or threonine in a polypeptide chain. Despite the chemical simplicity of the Tn antigen, its antigenic structure is considered to be complex and the clear determinants of Tn antigenicity remain poorly understood. As a consequence, a broad variety of anti-Tn monoclonal antibodies (mAbs) have been generated. To further investigate the nature and complexity of the Tn antigen, we generated seven different anti-Tn mAbs of IgM and IgG classes raised against human Jurkat T cells, which are Tn-positive due to the low activity of T-synthase and mutation in specific chaperone Cosmc. The binding analysis of anti-Tn mAbs with the array of synthetic saccharides, glycopeptides and O-glycoproteins revealed unexpected differences in specificities of anti-Tn mAbs. IgM mAbs bound the terminal GalNAc residue of the Tn antigen irrespective of the peptide context or with low selectivity to the glycoproteins. In contrast, IgG mAbs recognized the Tn antigen in the context of a specific peptide motif. Particularly, JA3 mAb reacted to the GSPP or GSPAPP, and JA5 mAb recognized specifically the GSP motif (glycosylation sites are underlined). The major O-glycan carrier proteins CD43 and CD162 and isoforms of CD45 expressed on Jurkat cells were precipitated by anti-Tn mAbs with different affinities. In summary, our data suggest that Tn antigen-Ab binding capacity is determined by the peptide context of the Tn antigen, antigenic specificity of the Ab and class of the immunoglobulin. The newly generated anti-Tn IgG mAbs with the strong specificity to glycoprotein CD43 can be particularly interesting for the application in leukemia diagnostics and therapy. PMID:22143985

  18. Analysis of Tn antigenicity with a panel of new IgM and IgG1 monoclonal antibodies raised against leukemic cells

    PubMed Central

    Blixt, Ola; Lavrova, Olga I; Mazurov, Dmitriy V; Cl, Emiliano; Kra?un, Stjepan K; Bovin, Nicolai V; Filatov, Alexander V

    2012-01-01

    CD175 or Tn antigen is a carbohydrate moiety of N-acetylgalactosamine (GalNAc)?1-O- linked to the residue of amino acid serine or threonine in a polypeptide chain. Despite the chemical simplicity of the Tn antigen, its antigenic structure is considered to be complex and the clear determinants of Tn antigenicity remain poorly understood. As a consequence, a broad variety of anti-Tn monoclonal antibodies (mAbs) have been generated. To further investigate the nature and complexity of the Tn antigen, we generated seven different anti-Tn mAbs of IgM and IgG classes raised against human Jurkat T cells, which are Tn-positive due to the low activity of T-synthase and mutation in specific chaperone Cosmc. The binding analysis of anti-Tn mAbs with the array of synthetic saccharides, glycopeptides and O-glycoproteins revealed unexpected differences in specificities of anti-Tn mAbs. IgM mAbs bound the terminal GalNAc residue of the Tn antigen irrespective of the peptide context or with low selectivity to the glycoproteins. In contrast, IgG mAbs recognized the Tn antigen in the context of a specific peptide motif. Particularly, JA3 mAb reacted to the GSPP or GSPAPP, and JA5 mAb recognized specifically the GSP motif (glycosylation sites are underlined). The major O-glycan carrier proteins CD43 and CD162 and isoforms of CD45 expressed on Jurkat cells were precipitated by anti-Tn mAbs with different affinities. In summary, our data suggest that Tn antigenAb binding capacity is determined by the peptide context of the Tn antigen, antigenic specificity of the Ab and class of the immunoglobulin. The newly generated anti-Tn IgG mAbs with the strong specificity to glycoprotein CD43 can be particularly interesting for the application in leukemia diagnostics and therapy. PMID:22143985

  19. Structure of fosfomycin resistance protein FosA from transposon Tn2921

    PubMed Central

    Pakhomova, Svetlana; Rife, Chris L.; Armstrong, Richard N.; Newcomer, Marcia E.

    2004-01-01

    The crystal structure of fosfomycin resistance protein FosA from transposon Tn2921 has been established at a resolution of 2.5 . The protein crystallized without bound Mn(II) and K+, ions crucial for efficient catalysis, providing a structure of the apo enzyme. The protein maintains the three-dimensional domain-swapped arrangement of the paired ?????-motifs observed in the genomically encoded homologous enzyme from Pseudomonas aeruginosa (PA1129). The basic architecture of the active site is also maintained, despite the absence of the catalytically essential Mn(II). However, the absence of K+, which has been shown to enhance enzymatic activity, appears to contribute to conformational heterogeneity in the K+-binding loops. PMID:15075406

  20. The Travis County, Texas, meteorites

    NASA Astrophysics Data System (ADS)

    McCoy, T. J.; Ehlmann, A. J.; Keil, K.

    1995-05-01

    Studies of 52 specimens recovered from the find site of the original Travis County meteorite reveal the presence of two distinct meteorites. Travis County (a), which includes the original Travis County meteorite, is the more abundant meteorite and is classified as an H5(S4) shock-blackened chondrite. Travis County (b) is classified as an H4(S2) chondrite with rare chondritic clasts of H group parentage, indicating that the meteorite is a breccia.

  1. TN-68 Spent Fuel Transport Cask Analytical Evaluation for Drop Events

    SciTech Connect

    Shah, M. J.; Klymyshyn, Nicholas A.; Adkins, Harold E.; Koeppel, Brian J.

    2007-03-30

    The U.S. Nuclear Regulatory Commission (NRC) is responsible for licensing commercial spent nuclear fuel transported in casks certified by NRC under the Code of Federal Regulations (10 CFR), Title 10, Part 71 [1]. Both the International Atomic Energy Agency regulations for transporting radioactive materials [2, paragraph 727], and 10 CFR 71.73 require casks to be evaluated for hypothetical accident conditions, which includes a 9-meter (m) (30-ft) drop-impact event onto a flat, essentially unyielding, horizontal surface, in the most damaging orientation. This paper examines the behavior of one of the NRC certified transportation casks, the TN-68 [3], for drop-impact events. The specific area examined is the behavior of the bolted connections in the cask body and the closure lid, which are significantly loaded during the hypothetical drop-impact event. Analytical work to evaluate the NRC-certified TN-68 spent fuel transport cask [3] for a 9-m (30-ft) drop-impact event on a flat, unyielding, horizontal surface, was performed using the ANSYS [4] and LS DYNA [5] finite-element analysis codes. The models were sufficiently detailed, in the areas of bolt closure interfaces and containment boundaries, to evaluate the structural integrity of the bolted connections under 9-m (30-ft) free-drop hypothetical accident conditions, as specified in 10 CFR 71.73. Evaluation of the cask for puncture, caused by a free drop through a distance of 1-m (40-in.) onto a mild steel bar mounted on a flat, essentially unyielding, horizontal surface, required by 10 CFR 71.73, was not included in the current work, and will have to be addressed in the future. Based on the analyses performed to date, it is concluded that, even though brief separation of the flange and the lid surfaces may occur under some conditions, the seals would close at the end of the drop events, because the materials remain elastic during the duration of the event.

  2. Cruise No.: TN-179, Leg 1 and Leg 3 April 8, 2005 FOCI No.: 1TT05 and 2TT05

    E-print Network

    , Alaskan Ocean Observatory System, Steller Sea Lion Research Programs, and PMEL/FOCI base. Leg 1.4.2 Cruise TN-179 Leg 2 Gulf of Alaska. 1.4.3 Cruise TN-179 Leg 3 Eastern Bering Sea shelf. 2.0 CRUISE of the North Pacific Ocean and Bering Sea in order to discern the physical and biological processes

  3. Skeletal Muscle Troponin I (TnI) in Animal Fat Tissues to Be Used as Biomarker for the Identification of Fat Adulteration

    PubMed Central

    Park, Bong-Sup; Oh, Young-Kyoung; Kim, Min-Jin

    2014-01-01

    In this study, the existence of skeletal muscle troponin I (smTnI), well-known as a muscle protein in fat tissues, and the utilization of smTnI as a biomarker for the identification of fat adulteration were investigated. A commercial antibody (ab97427) specific to all of animals smTnI was used in this study. Fat and meat samples (cooked and non-cooked) of pork and beef, and chicken considered as representative meats were well minced and extracted by heating and non-heating methods, and the extracts from fat and meat tissues were probed by the antibody used in both enzyme-linked immunosorbent assay (ELISA) and immunoblot. The antibody exhibited a strong reaction to all meat and fat extracts in ELISA test. On the other hand, the results of immunoblot analsis revealed a 23 kDa high intensity band corresponding to the molecular weight of smTnI (23786 Da). These results demonstrate that the existence of smTnI in all animal fat tissues. Since there are monoclonal antibodies specific to each species smTnI, smTnI in fat tissues could be used as a biomarker to identify or determine animal species adulterated in meat products. Therefore, an analytical method to identify fraudulent fat adulteration can be developed with an antibody specific to each species smTnI.

  4. PHYSICAL LINKAGE OF MULTIPLE DRUG RESISTANCE TRANSPOSON TN21 TO COLICIN PLASMIDS AND DISINFECTANT RESISTANCE GENE MERB IN AVIAN ESCHERICHIA COLI

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Recently, we have reported on the widespread distribution of multiple-drug resistance transposon, Tn21 among clinical, avian Escherichia coli isolates in North Georgia. Tn21 is a 21 kb transposon that contains an integron, a mobile genetic element that captures antibiotic resistance gene(s), strep...

  5. Agenda for Sept 10-11 workshop in Oak Ridge, TN Sustainability of Bioenergy Systems: Cradle to Grave

    E-print Network

    Pennycook, Steve

    Agenda for Sept 10-11 workshop in Oak Ridge, TN Sustainability of Bioenergy Systems: Cradle for bioenergy Donna Perla (EPA) Paul Leiby (ORNL) o Components of sustainability to consider relevant to bioenergy Rebecca Efroymson (ORNL) - A Framework for Selecting Sustainability Indicators for Bioenergy

  6. 76 FR 34799 - Permanent Dam Safety Modification at Cherokee, Fort Loudoun, Tellico, and Watts Bar Dams, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-14

    ... Permanent Dam Safety Modification at Cherokee, Fort Loudoun, Tellico, and Watts Bar Dams, TN AGENCY... various alternatives for permanent modifications to the existing dam facilities at Cherokee, Fort Loudoun, Tellico, and Watts Bar dams in Tennessee. The level of review will be determined after the public...

  7. Identification of a catabolic transposon, Tn4371, carrying biphenyl and 4-chlorobiphenyl degradation genes in Alcaligenes eutrophus A5.

    PubMed Central

    Springael, D; Kreps, S; Mergeay, M

    1993-01-01

    Alcaligenes eutrophus A5 catabolizes biphenyl to CO2 via benzoate and 4-chlorobiphenyl to 4-chlorobenzoate. In curing and conjugation experiments, the A5 endogenous 51-kb IncP1 plasmid pSS50 was found to be dispensable for biphenyl and 4-chlorobiphenyl catabolism. Transfer of the biphenyl- and 4-chlorobiphenyl-degrading phenotype by means of pSS50 was observed at a frequency of 10(-5) per transferred plasmid in matings of A5 with other A. eutrophus strains. Transconjugants harbor enlarged pSS50 derivatives which contain additional genetic information governing the oxidation of biphenyl and 4-chlorobiphenyl to benzoate and 4-chlorobenzoate and originating from the chromosome of strain A5. The following observations indicate that the catabolic genes reside on a 59-kb large transposon (Tn4371) for which a restriction map is presented. (i) Tn4371 transposes between different replicons and at different locations of the same replicon. (ii) Transposition was observed in a Rec- strain of A. eutrophus. (iii) Tn4371 transposes as a single, contiguous piece of DNA. Although an RP4::Tn4371 plasmid was stably maintained in different hosts, the plasmid conferred growth on biphenyl only when present in strains of A. eutrophus and in an Acinetobacter sp. strain. Images PMID:8383664

  8. RADIOCARBON MEASUREMENT OF THE BIOGENIC CONTRIBUTION TO SUMMERTIME PM 2.5 AMBIENT AEROSOL IN NASHVILLE, TN

    EPA Science Inventory

    Radiocarbon (14C) measurements performed on PM-2.5 samples collected near Nashville, TN from June 21 to July 13, 1999, showed high levels of modern carbon, ranging from 56 to 80% of the total carbon in the samples. Radiocarbon measurements performed on dichloromethane extracts of...

  9. 75 FR 6636 - Foreign-Trade Zone 77-Memphis, TN Application for Subzone Cummins, Inc. (Engine Components...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-10

    ... distribution of foreign and domestic-origin internal combustion engine (diesel and CNG) parts and components...-Trade Zones Board Foreign-Trade Zone 77--Memphis, TN Application for Subzone Cummins, Inc. (Engine... internal-combustion engine parts warehousing and distribution facility of Cummins, Inc. (Cummins),...

  10. 77 FR 73978 - Foreign-Trade Zone 148-Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-12

    ...), located in Rockwood, Tennessee, with authority to manufacture carbon fiber for export and oxidized polyacrylonitrile fiber (Board Order 1868, 77 FR 69435, 11/19/2012). Board Order 1868 did not include authority to...-Trade Zones Board Foreign-Trade Zone 148--Knoxville, TN, Toho Tenax America, Inc. (Carbon...

  11. Arbitrary PCR for Rapid Mapping of Tn5 Insertions in Pyoverdine Genes of Pseudomonas fluorescens Pf-5

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A collection of 13 transposon mutants deficient in pyoverdine production was analyzed using an arbitrary polymerase chain reaction (PCR) approach to map the sites of Tn5 insertions in the genome of Pseudomonas fluorescens Pf-5. The arbitrary PCR method involved two rounds of reactions, with the fi...

  12. Mobility and Generation of Mosaic Non-Autonomous Transposons by Tn3-Derived Inverted-Repeat Miniature Elements (TIMEs)

    PubMed Central

    Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

    2014-01-01

    Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element captured with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not only of transposons and plasmids, but also of other types of mobile genetic elements. PMID:25121765

  13. Humboldt County Employer Survey.

    ERIC Educational Resources Information Center

    Lyons, Dave

    A project was undertaken in Humboldt County to collect information from large and small businesses in the areas of agriculture, mining, manufacturing, transportation, wholesale and retail, finance, services, and public information with respect to their employee requirements and needs. In all, 451 firms were surveyed to determine the size of the

  14. [City and County Records.

    ERIC Educational Resources Information Center

    Combs, Judith O.; And Others

    Six papers presented at the Institute were concerned with city and county records. They are: "EWEB and Its Records," which discusses the history, laws and records of the Eugene Water and Electric Board (EWEB);""Police Records: Eugene, Oregon," classifies police records, other than administrative, into three general categories: (1) case or

  15. 78 FR 43827 - Irish Potatoes Grown in Modoc and Siskiyou Counties, California, and in All Counties in Oregon...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-22

    ...Siskiyou Counties, California, and in All Counties in Oregon, Except Malheur County...Siskiyou Counties, California, and in all counties in Oregon, except Malheur County...at: http://www.regulations.gov. All comments submitted in response to this...

  16. 75 FR 49016 - County of Greenville, S.C.-Acquisition Exemption-Greenville County Economic Development Corporation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ...Exemption--Greenville County Economic Development Corporation The County of...acquire from Greenville County Economic Development Corporation (GCEDC) approximately...See Greenville County Economic Development...

  17. 77 FR 72968 - Revisions to the California State Implementation Plan, for Imperial County, Placer County and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-07

    ...Placer County and Ventura County Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD)...

  18. 77 FR 73005 - Revisions to the California State Implementation Plan, Imperial County, Placer County, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-07

    ...Placer County, and Ventura County Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD)...

  19. 75 FR 26709 - Clarke County Water Supply Project, Clarke County, IA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ...Resources Conservation Service Clarke County Water Supply Project, Clarke County, IA...being prepared for the Clarke County Water Supply Project, Clarke County, Iowa...comments by NRCS information related to water supply demand requirements for...

  20. pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

    SciTech Connect

    Matsumoto, Yasuyuki; Zhang, Qing; Akita, Kaoru; Nakada, Hiroshi; Hamamura, Kazunori; Tokuda, Noriyo; Tsuchida, Akiko; Noguchi Institute, 1-8-1 Kaga, Itabashi, Tokyo 173-0003 ; Matsubara, Takeshi; Hori, Tomoko; Okajima, Tetsuya; Furukawa, Keiko; Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, 1200 Matsumoto-cho, Kasugai 487-8501 ; Urano, Takeshi; Furukawa, Koichi

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer ppGalNAc-T13 was up-regulated in high metastatic sublines of Lewis lung cancer. Black-Right-Pointing-Pointer ppGalNAc-T13 expression enhanced cell invasion activity in low metastatic sublines. Black-Right-Pointing-Pointer Trimeric Tn antigen was induced in the transfectant cells of ppGalNAc-T13 cDNA. Black-Right-Pointing-Pointer A major protein carrying trimeric Tn structure was identified as Syndecan-1. Black-Right-Pointing-Pointer Silencing of ppGalNAc-T13 resulted in the reduction of invasion and of metastasis.. -- Abstract: In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. These sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated by subcutaneous injection of T13-KD clones showed lower coalescence to fascia and peritoneum, and significantly reduced lung metastasis than control clones. These data suggested that high expression of pp-GalNAc-T13 gene generated trimeric Tn antigen on Syndecan-1, leading to the enhanced metastasis.

  1. Insertions of mini-Tn10 transposon T-POP in Salmonella enterica sv. typhi.

    PubMed Central

    Hidalgo, Alejandro A; Trombert, A Nicole; Castro-Alonso, J C; Santiviago, Carlos A; Tesser, Bruno R; Youderian, Philip; Mora, Guido C

    2004-01-01

    We have mutagenized a clinical strain of Salmonella enterica sv. typhi with mini-transposon Tn10dTet (T-POP) to obtain conditional lethal (tetracycline-dependent) mutants with T-POP insertions upstream of essential genes. Generalized transducing phage P22 was used to introduce T-POP from a S. typhimurium donor into a S. typhi recipient. Chromosomal DNA was purified from the mutagenized donor strains, fragmented, and then electroporated into S. typhi to backcross the original T-POP insertions. Four tetracycline-dependent mutants with two distinct terminal phenotypes were found among 1700 mutants with T-POP insertions. When grown in the absence of tetracycline, two of the four tetracycline-dependent mutants arrest at a late stage in the cell cycle, can be rescued by outgrowth in media with tetracycline, and define a reversible checkpoint late in the cell cycle. One of these insertions creates an operon fusion with a gene, yqgF, that is conserved among gram-negative bacteria and likely encodes an essential Holliday junction resolvase. T-POP insertions can be used not only to identify essential S. typhi genes but also to reveal novel phenotypes resulting from the depletion of their products. PMID:15280224

  2. Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida.

    PubMed

    Zobel, Sebastian; Benedetti, Ilaria; Eisenbach, Lara; de Lorenzo, Victor; Wierckx, Nick; Blank, Lars M

    2015-12-18

    The soil bacterium Pseudomonas putida is increasingly attracting considerable interest as a platform for advanced metabolic engineering through synthetic biology approaches. However, genomic context, gene copy number, and transcription/translation interplay often introduce considerable uncertainty to the design of reliable genetic constructs. In this work, we have established a standardized heterologous expression device in which the promoter strength is the only variable; the remaining parameters of the flow have stable default values. To this end, we tailored a mini-Tn7 delivery transposon vector that inserts the constructs in a single genomic locus of P. putida's chromosome. This was then merged with a promoter insertion site, an unvarying translational coupler, and a downstream location for placing the gene(s) of interest under fixed assembly rules. This arrangement was exploited to benchmark a collection of synthetic promoters with low transcriptional noise in this bacterial host. Growth experiments and flow cytometry with single-copy promoter-GFP constructs revealed a robust, constitutive behavior of these promoters, whose strengths and properties could be faithfully compared. This standardized expression device significantly extends the repertoire of tools available for reliable metabolic engineering and other genetic enhancements of P. putida. PMID:26133359

  3. Conformational toggling controls target site choice for the heteromeric transposase element Tn7

    PubMed Central

    Shi, Qiaojuan; Straus, Marco R.; Caron, Jeremy J.; Wang, Huasheng; Chung, Yu Seon; Guarn, Alba; Peters, Joseph E.

    2015-01-01

    The bacterial transposon Tn7 facilitates horizontal transfer by directing transposition into actively replicating DNA with the element-encoded protein TnsE. Structural analysis of the C-terminal domain of wild-type TnsE identified a novel protein fold including a central V-shaped loop that toggles between two distinct conformations. The structure of a robust TnsE gain-of-activity variant has this loop locked in a single conformation, suggesting that conformational flexibility regulates TnsE activity. Structure-based analysis of a series of TnsE mutants relates transposition activity to DNA binding stability. Wild-type TnsE appears to naturally form an unstable complex with a target DNA, whereas mutant combinations required for large changes in transposition frequency and targeting stabilized this interaction. Collectively, our work unveils a unique structural proofreading mechanism where toggling between two conformations regulates target commitment by limiting the stability of target DNA engagement until an appropriate insertion site is identified. PMID:26384427

  4. Identification of multiple mercury sources to stream sediments near Oak Ridge, TN, USA.

    PubMed

    Donovan, Patrick M; Blum, Joel D; Demers, Jason D; Gu, Baohua; Brooks, Scott C; Peryam, John

    2014-04-01

    Sediments were analyzed for total Hg concentration (THg) and isotopic composition from streams and rivers in the vicinity of the Y-12 National Security Complex (Y12) in Oak Ridge, TN (USA). In the stream directly draining Y12, where industrial releases of mercury (Hg) have been documented, high THg (3.26 to 60.1 ?g/g) sediments had a distinct Hg isotopic composition (?(202)Hg of 0.02 0.15 and ?(199)Hg of -0.07 0.03; mean 1SD, n = 12) compared to sediments from relatively uncontaminated streams in the region (?(202)Hg = -1.40 0.06 and ?(199)Hg of -0.26 0.03; mean 1SD, n = 6). Additionally, several streams that are nearby but do not drain Y12 had sediments with intermediate THg (0.06 to 0.21 ?g/g) and anomalous ?(202)Hg (as low as -5.07). We suggest that the low ?(202)Hg values in these sediments provide evidence for the contribution of an additional Hg source to sediments, possibly derived from atmospheric deposition. In sediments directly downstream of Y12 this third Hg source is not discernible, and the Hg isotopic composition can be largely explained by the mixing of low THg sediments with high THg sediments contaminated by Y12 discharges. PMID:24588770

  5. Conformational toggling controls target site choice for the heteromeric transposase element Tn7.

    PubMed

    Shi, Qiaojuan; Straus, Marco R; Caron, Jeremy J; Wang, Huasheng; Chung, Yu Seon; Guarn, Alba; Peters, Joseph E

    2015-12-15

    The bacterial transposon Tn7 facilitates horizontal transfer by directing transposition into actively replicating DNA with the element-encoded protein TnsE. Structural analysis of the C-terminal domain of wild-type TnsE identified a novel protein fold including a central V-shaped loop that toggles between two distinct conformations. The structure of a robust TnsE gain-of-activity variant has this loop locked in a single conformation, suggesting that conformational flexibility regulates TnsE activity. Structure-based analysis of a series of TnsE mutants relates transposition activity to DNA binding stability. Wild-type TnsE appears to naturally form an unstable complex with a target DNA, whereas mutant combinations required for large changes in transposition frequency and targeting stabilized this interaction. Collectively, our work unveils a unique structural proofreading mechanism where toggling between two conformations regulates target commitment by limiting the stability of target DNA engagement until an appropriate insertion site is identified. PMID:26384427

  6. Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system.

    PubMed

    Wickham, T J; Nemerow, G R

    1993-01-01

    A novel insect cell line from Trichoplusia ni, BTI-Tn 5B1-4 (Tn 5), was compared to Spodoptera frugiperda, Sf 9, cells for production of two recombinant secreted proteins: truncated Epstein-Barr viral attachment protein (EBV gp105) and truncated, soluble tissue factor (sTF). Under optimum conditions for both cell lines, Tn 5 cells produced 28-fold more secreted sTF than Sf 9 cells, respectively, on a per cell basis. The total production of gp105 was similar for the two cell lines. However, Tn5 cells secreted gp105 much more efficiently, resulting in 5-fold higher levels in the extracellular medium. Despite these increases, Tn 5 cells are attachment-dependent, and protein production is sensitive to the cell density (cells/cm2), unlike the Sf9 cell line which can be easily grown and scaled up in cell suspension cultures without significantly affecting its per cell production. Thus, protein production from Tn 5 cells above 0.1 L scales was optimized with respect to cell density using standard techniques for the growth of attachment-dependent cells. Roller bottles precoated with DEAE-based microcarriers and suspension cultures employing collagen-coated microcarriers were found to be effective ways of culturing Tn 5 cells. Predetermined optimal cell densities were used to produce EBV gp105 in microcarrier-coated roller bottles or in suspension cultures using collagen-coated microcarriers at concentrations close to those observed in tissue culture flasks. PMID:7764044

  7. SIMCOE COUNTY 2001 TRAFFIC ZONES

    E-print Network

    Toronto, University of

    SIMCOE COUNTY CITY OF BARRIE B.51 CITY OF ORILLIA B.52 2001 TRAFFIC ZONES SIMCOE COUNTY ! B.7 B.46 FORCES BASE BORDEN TINY/FLOS TOW NLINE C O U N TY R D . 10 3743 2001 TRAFFIC ZONES SIMCOE COUNTY B.47 B RD. 90 HWY 11 HORSESHOE VALLEY RD. HW Y. 26 HWY. 26 TINY/FLOS TOWNLINE 2001 TRAFFIC ZONES SIMCOE

  8. Simcoe County 2006 TRAFFIC ZONES

    E-print Network

    Toronto, University of

    Simcoe County 2006 TRAFFIC ZONES SIMCOE COUNTY B.7 City of Barrie City of Orillia B.49 B.50 B.51 B 8601 8571 8572 8604 8667 8665 8575 8574 8578 8577 8573 8576 8646 8645 8612 2006 TRAFFIC ZONES SIMCOE 8583 8584 8657 8603 8660 8649 8582 8648 8647 8650 2006 TRAFFIC ZONES SIMCOE COUNTY B.50 B.53 B.51B.49 6

  9. Loss of catalase activity in Tn1545-induced mutants does not reduce growth of Listeria monocytogenes in vivo.

    PubMed Central

    Leblond-Francillard, M; Gaillard, J L; Berche, P

    1989-01-01

    Two catalase-negative mutants of Listeria monocytogenes were obtained by chromosomal insertions of the conjugative transposon Tn1545. The loss of catalase activity did not reduce the level of virulence of these mutants in mice. Indeed, both mutants were capable of growing in host tissues at the same rate as the parental catalase-positive strain. These results favor the view that catalase does not play a critical role in the resistance of L. monocytogenes to macrophage killing. PMID:2545629

  10. Berkeley County School District Rodney Thompson

    E-print Network

    Kunkle, Tom

    Berkeley County School District Rodney Thompson Chief Administrative Officer TO: Institutions, Superintendent DATE: October 1, 2010 SUBJECT: Research Request and Undergraduate Studies The Berkeley County standards or may request additional information. (Based on the volume of request, Berkeley County employees

  11. Testing and analyses of the TN-24P PWR spent-fuel dry storage cask loaded with consolidated fuel

    SciTech Connect

    McKinnon, M A; Michener, T E; Jensen, M F; Rodman, G R

    1989-02-01

    A performance test of a Transnuclear, Inc. TN-24P storage cask configured for pressurized water reactor (PWR) spent fuel was performed. The work was performed by the Pacific Northwest Laboratory (PNL) and Idaho National Engineering Laboratory (INEL) for the US Department of Energy Office of Civilian Radioactive Waste Management (OCRWM) and the Electric Power Research Institute. The performance test consisted of loading the TN-24P cask with 24 canisters of consolidated PWR spent fuel from Virginia Power's Surry and Florida Power and Light's Turkey Point reactors. Cask surface and fuel canister guide tube temperatures were measured, as were cask surface gamma and neutron dose rates. Testing was performed with vacuum, nitrogen, and helium backfill environments in both vertical and horizontal cask orientations. Transnuclear, Inc., arranged to have a partially insulated run added to the end of the test to simulate impact limiters. Limited spent fuel integrity data were also obtained. From both heat transfer and shielding perspectives, the TN-24P cask with minor refinements can be effectively implemented at reactor sites and central storage facilities for safe storage of unconsolidated and consolidated spent fuel. 35 refs., 93 figs., 17 tabs.

  12. A fluorogenic heterogeneous immunoassay for cardiac muscle troponin cTnI on a digital microfluidic device.

    PubMed

    Tsaloglou, Maria-Nefeli; Jacobs, Adrian; Morgan, Hywel

    2014-09-01

    We describe a fluorogenic two-site noncompetitive heterogeneous immunoassay with magnetic beads on a low-voltage digital microfluidic platform using closed electrowetting-on-dielectric (EWOD). All the steps of an enzyme-linked immunosorbent assay (ELISA) were performed on the device using 9H-(1, 3-dichloro-9, 9-dimethylacridin-2-one-7-yl) phosphate as the fluorogenic substrate for the enzyme alkaline phosphatase. The performance of the system was demonstrated with cardiac marker Troponin I (cTnI) as a model analyte in phosphate-buffered saline samples. cTnI was detected within the diagnostically relevant range with a limit of detection of 2.0ng/mL (CV?=?6.47%). Washing of magnetic beads was achieved by movement through a narrow region of buffer bridging one drop to another with minimal fluid transfer. More than 90% of the unbound reagents were removed after five washes. Further experiments testing human blood serum on the same platform demonstrated a sample-to-answer time at ?18.5min detecting 6.79ng/mL cTnI. PMID:25074544

  13. Taiwan Nantou County earthquake 0327 Taiwan Nantou County earthquake

    E-print Network

    Chen, Sheng-Wei

    Taiwan Nantou County earthquake 20130327 1 #12;0327 Taiwan Nantou County earthquake Source, Intensity 5 #12;I II III IV V VI VII Intensity Shake map of the March 27 Earthquake The peak ground and Technology Center for Disaster Reduction (NCDR) #12;Earthquake Response and Evacuation are a Part of Students

  14. Bridging Brown County Case Study Bridging Brown County Case Study

    E-print Network

    Minnesota, University of

    . But we also belong to a larger rural community: the community formed by all residents of Brown County. We and Finding a Future Summary and Background Communities in the rural Midwest typically understand and solve unique issues facing rural communities? In Brown County, Minnesota, the community developed

  15. Visible-near infrared reflectance spectroscopy for estimating soil total nitrogen contents in the Sanjiang Yuan Regions, China: a case study of Yushu County and Maduo County, Qinghai province

    NASA Astrophysics Data System (ADS)

    Gao, Xiaohong; Yang, Yang; Zhang, Wei; Jia, Wei; Li, Jianshan; Tian, Chengming; Zhang, Yanjiao; He, Linhua

    2014-11-01

    The objective of this paper was to evaluate the prediction ability of visible and near-infrared reflectance spectroscopy(VNIRP) to estimate soil total nitrogen concentration (TN) in the Sanjiang Yuan regions, Qinghai province, China by using partial least squares regression (PLSR) method. A total of 149 soil samples (0-30cm) were collected respectively from Yushu county and Maduo county in August 2012. The soil total nitrogen concentrations were measured using Vario EL III elemental analyzer (Germany Elementar Inc), and the soil spectral reflectance of with spectral range 350-2500nm was measured using ASD FieldSpec 4 in the laboratory. PLSR model was used to relate the reflectance spectra and its five different pre-processing transformations including the first order derivative reflectance (FDR), the second order derivative reflectance(SDR), the logarithm of the inverse of the reflectance (Log (1/R)), the depth of the band (Band depth) and the first order derivative of the logarithm of reflectance (( Log R)') to the soil total nitrogen contents measured. The results showed that the combination of visible-near infrared reflectance spectroscopy and PLSR to estimate TN produced a good prediction ability with R2 < 0.76 and RPD < 2.01 in the Sanjiang Yuan regions. The best prediction ability was obtained by (Log R) ' with R2CV = 0.87, R2V =0.88 and RPD <2.67, followed by Log (1/R), Band Depth, SDR, FDR and Reflectance. Visible and near infrared spectroscopy provided a rapid and effective method for estimating soil TN from five different soil types in the Sanjiang Yuan Regions, Qinghai province.

  16. Transposon Tn5281 is the main distributor of the aminoglycoside modifying enzyme gene among isolates of Enterococcus faecalis in Tehran hospitals.

    PubMed

    Feizabadi, Mohammad Mehdi; Shokrzadeh, Leila; Sayady, Sara; Asadi, Soroor

    2008-10-01

    Infections with high levels of gentamicin-resistant (HLGR) isolates of Enterococcus faecalis are common in Tehran hospitals. Genes encoding such resistance are transmissible by conjugation at high frequency. The purpose of this study was to determine the existence of Tn5281 and its flanking aminoglycoside modifying enzyme gene aac(6')-aph(2") among 102 HLGR isolates of E. faecalis cultured from patients at three hospitals in Tehran, Iran. These isolates were detected by disks containing 120 microg of gentamicin and made 65% of all E. faecalis during the study period. DNA was extracted from HLGR isolates and subjected to PCR assays targeting aac(6')-aph(2") and conjugative transposon Tn5281. The amplified aac(6')-aph(2") gene was labeled with digoxigenin and probed with Tn5281 amplicons in dot blot hybridization assays. The aac(6')-aph(2") gene was detected in 91%-92% (n = 93) of the HLGR isolates. All isolates containing aac(6')-aph(2") were positive in long-PCR targeting Tn5281 and the probe hybridized with Tn5281 amplicons. The number of HLGR isolates of E. faecalis has increased considerably in Tehran hospitals. Tn5281 is the main cause of transmission of aac(6')-aph(2") to different isolates of E. faecalis in the hospitals studied. PMID:18923558

  17. Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition

    SciTech Connect

    Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi; Matsuura, Masaaki; Gomi, Yasuyuki; Takahashi, Michiaki; Yamanishi, Koichi; Mori, Yasuko

    2010-06-20

    Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZ{alpha}-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cells as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.

  18. Groundwater Transport of Organic Compounds in Old Salvage Yard, Oak Ridge, TN - 12089

    SciTech Connect

    Malek-Mohammadi, Siamak; Tachiev, Georgio; Roelant, David; Bostick, Kent; Daniel, Anamary

    2012-07-01

    In 1950's and early 1960's during production of nuclear weapons at the US Department of Energy Y-12 National Security Complex in Oak Ridge TN, volatile organic compounds (VOCs) as well as heavy metals, nitrates, and radionuclides were released to the environment. Field investigations revealed that much of this contamination is still present in soil, bedrock, and groundwater. Operational buildings and old disposal facilities at the site have been identified as major sources of contamination. The Old Salvage Yard (OSY) on the western side of the site has long been characterized as the major source of VOC contamination in soil and groundwater. In order to analyze the fate and transport of VOC contamination- including tetrachloroethene (PCE), 1,2- dichloroethene (1,2-DCE), cis-1,2-dichloroethene (cis-1,2-DCE) and vinyl chloride (VC) - in groundwater and soil at the vicinity of OSY, an integrated surface and subsurface flow and transport model has been developed for the Y-12 NSC using the hydrodynamic and transport numerical package, MIKE-SHE. Hydrogeological characteristics of the site such as hydraulic conductivity, and transport parameters such as partitioning coefficients were varied in an effort to delineate subsurface flow and transport pathways, potential downstream impacts on Upper East Fork Poplar Creek, and the potential risk to industrial workers involved in related Decontamination and Decommissioning (D and D) activities. The simulation results were compared with the analytical modeling results previously performed by McLane Environmental Inc. using SESOIL-AT123D. Specific simulations have been performed to investigate the effect of possible remedial action (removing the contaminated surface soil layers) on the fate and transport of VOCs. The results of the MIKE-SHE reported here can be considered as an upper limit for the predicted concentrations. Based on MIKE-SHE results, PCE, 1,2 DCE, cis-1,2-DCE, and VC are sources in soil with potential to equal or exceed industrial groundwater hazard and risk levels at the vicinity of OSY. VOC contaminants in soil and groundwater will decay below industrial groundwater risk and hazard levels within approximately 20 years. Excavation of surface soil layers at the site will considerably reduce the concentration of VOCs in groundwater and the possibility of migration of VOCs to surface waters. (authors)

  19. 75 FR 22100 - Nevada County and Placer County, CA, Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-27

    ... Forest Service Nevada County and Placer County, CA, Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Nevada County and Placer County Resource Advisory... National Forests in Nevada and Placer Counties. DATES: The meeting will be held Tuesday, May 4, 2010 at...

  20. San Jacinto County Jail Building

    E-print Network

    Raiford Stripling Associates, Inc.; Stripling, Raiford L.

    1973-01-01

    in the 1880s for indigent people. Housing and land for gardens were provided to those persons with no income. Also, this area contained a two-story brick jail for county prisoners and the mentally ill. The cemetery became the final resting place... dating to the 19th and 20th centuries. The Navarro County Cemetery was established in the 1880s and is the final resting place for indigent persons, mentally ill patients, and prisoners buried by the county. These sites will be avoided by the planned...

  1. 75 FR 25308 - Environmental Impact Statement: Winnebago County, IL and Rock County, WI

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ...Administration Environmental Impact Statement: Winnebago County, IL and Rock County, WI AGENCY: Federal Highway Administration (FHWA...Wisconsin Route 213 and Nye School Road northwest of Beloit, Rock County, Wisconsin to the interchange of Rockton Road and...

  2. The Ada County Internship Program.

    ERIC Educational Resources Information Center

    Tallmon, Jim; Carter, Kay

    1991-01-01

    Describes Ada County (Idaho) Juvenile Courts' internship program in which undergraduate and graduate students participate in sponsored work experience in juvenile detention settings. Outlines recruitment, budget, placement, expectations of students and institutions, supervision, evaluation, termination, and grievance procedures. (SK)

  3. VERMONT COUNTY HEALTH DATA2

    EPA Science Inventory

    This datalayer contains Vermont Population and Health data describing public health (1986-2000), by county, extracted from various sources, such as; the Vermont Department of Health, the Vermont Center for Justice Research, the National Institute on Alcohol Abuse & Alcoholis...

  4. Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi.

    PubMed Central

    Kotoujansky, A; Lemattre, M; Boistard, P

    1982-01-01

    A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled by this episome (Lac+, Tetr, Tra+) were expressed, and its replication was stopped at 40 degrees C and above. At 30 degrees C, the episome was easily transferred between strains derived from E. carotovora subsp. chrysanthemi 3937j and to E coli. Hfr donor strains were obtained from a F' strain of 3937j by selecting clones which grew at 40 degrees C on plates containing tetracycline. One of these strains, Hfrq, was examined in more detail: the characters Lac+ and Tetr were stabilized and did not segregate higher than its parental F' strain. The mating was most efficient at 37 degrees C on a membrane. Hfrq transferred its chromosome to recipient strains at high frequency and in a polarized fashion, as evidenced by the gradient of transfer frequencies, the kinetics of marker entry (in interrupted mating experiments), and the analysis of linkage between different markers. The chromosome of Hfrq was most probably transferred in the following sequence: origin...met...xyl...arg...ile...leu...thr...cys...pan...ura...gal...trp...his. ..pur... Moreover, this genetic transfer system proved to be efficient in strain construction. PMID:6277860

  5. Efficient role of BacTN635 on the safety properties, sensory attributes, and texture profile of raw minced meat beef and chicken breast.

    PubMed

    Smaoui, S; Elleuch, L; Ben Salah, R; Najah, S; Chakchouk-Mtibaa, A; Sellem, I; Besbes, S; Mellouli, L

    2014-01-01

    Bacteriocin BacTN635, produced by Lactobacillus plantarum sp. TN635, was purified and characterised in previous work. In this study we report the biotechnological application of this bacteriocin as a biopreservative during storage at 4C of raw minced meat beef and chicken breast. Overall, the results obtained showed that the addition of the semi-purified BacTN635 at 500 or 1000 AU g(-1) in raw minced meat beef and chicken breast can delay the proliferation of spoilage microorganisms, suppress the growth of the pathogenic microorganism Listeria monocytogenes, improve sensory quality, texture attributes, and extend the shelf-life of these two meat products during refrigerated storage. BacTN635 at 1000 AU g(-1) could extend the shelf-life, and the meat showed good sensory characteristics. Therefore, treatment with semi-purified BacTN635 can be used as a safe method for preservation of raw minced meat beef and chicken breast. PMID:24320082

  6. Liberty County State of the Community

    E-print Network

    Tedford, Emily; Gao, Fan; Reja, Md Yousuf

    2015-01-01

    Tyler County and parts of what later became Hardin, Chambers, San Jacinto, and Polk counties. The Countys early development focused on agriculture, especially timber production, since timber could be floated down the Trinity River to Houston-area ports... and part of the Houston metropolitan area. The County is bisected by the Trinity River from North to South and US Highway 90 from East to West. The County has a very strategic location, along the Trinity River, allowing access to the Port to the South...

  7. Excision of Tn10 from the donor site during transposition occurs by flush double-strand cleavages at the transposon termini.

    PubMed Central

    Benjamin, H W; Kleckner, N

    1992-01-01

    Tn10 transposition is accomplished without extensive replication of the transposon sequences. Replicative cointegrate formation is precluded by efficient separation of transposon sequences from flanking donor DNA at an early stage in the transposition reaction. We report here that excision of Tn10 from its donor site occurs by a pair of flush double-strand breaks. Breaks occur at each end of the element precisely between the terminal base pair of the element and the first base pair of flanking DNA. This observation provides definitive evidence that cleavage of both strands of the element occurs under the direct control of Tn10 transposase protein. It is highly likely that transposase itself is directly responsible for these cleavages. The implications of this possibility are discussed. Images PMID:1316613

  8. A Role for Tn6029 in the Evolution of the Complex Antibiotic Resistance Gene Loci in Genomic Island 3 in Enteroaggregative Hemorrhagic Escherichia coli O104:H4

    PubMed Central

    Chowdhury, Piklu Roy; Charles, Ian G.; Djordjevic, Steven P.

    2015-01-01

    In enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104 the complex antibiotic resistance gene loci (CRL) found in the region of divergence 1 (RD1) within E. coli genomic island 3 (GI3) contains blaTEM-1, strAB, sul2, tet(A)A, and dfrA7 genes encoding resistance to ampicillin, streptomycin, sulfamethoxazole, tetracycline and trimethoprim respectively. The precise arrangement of antibiotic resistance genes and the role of mobile elements that drove the evolutionary events and created the CRL have not been investigated. We used a combination of bioinformatics and iterative BLASTn searches to determine the micro-evolutionary events that likely led to the formation of the CRL in GI3 using the closed genome sequences of EAHEC O104:H4 strains 2011C-3493 and 2009EL-2050 and high quality draft genomes of EAHEC E. coli O104:H4 isolates from sporadic cases not associated with the initial outbreak. Our analyses indicate that the CRL in GI3 evolved from a progenitor structure that contained an In2-derived class 1 integron in a Tn21/Tn1721 hybrid backbone. Within the hybrid backbone, a Tn6029-family transposon, identified here as Tn6029C abuts the sul1 gene in the 3-Conserved Segment (-CS) of a class 1 integron generating a unique molecular signature that has only previously been observed in pASL01a, a small plasmid found in commensal E. coli in West Africa. From this common progenitor, independent IS26-mediated events created two novel transposons identified here as Tn6029D and Tn6222 in 2011C-3493 and 2009EL-2050 respectively. Analysis of RD1 within GI3 reveals IS26 has played a crucial role in the assembly of regions within the CRL. PMID:25675217

  9. A TALE of Transposition: Tn3-Like Transposons Play a Major Role in the Spread of Pathogenicity Determinants of Xanthomonas citri and Other Xanthomonads

    PubMed Central

    Ferreira, Rafael Marini; de Oliveira, Amanda Carolina P.; Moreira, Leandro M.; Belasque, Jos; Gourbeyre, Edith; Siguier, Patricia; Ferro, Maria Ins T.; Ferro, Jesus A.

    2015-01-01

    ABSTRACT Members of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomonas citri plasmid pXAC64. These are flanked by short inverted repeats (IRs). The region was identified as a transposon, TnXax1, with typical Tn3 family features, including a transposase and two recombination genes. Two 14-bp palindromic sequences within a 193-bp potential resolution site occur between the recombination genes. Additional derivatives carrying different T3SEs and other passenger genes occur in different Xanthomonas species. The T3SEs include transcription activator-like effectors (TALEs). Certain TALEs are flanked by the same IRs as found in TnXax1 to form mobile insertion cassettes (MICs), suggesting that they may be transmitted horizontally. A significant number of MICs carrying other passenger genes (including a number of TALE genes) were also identified, flanked by the same TnXax1 IRs and delimited by 5-bp target site duplications. We conclude that a large fraction of T3SEs, including individual TALEs and potential pathogenicity determinants, have spread by transposition and that TnXax1, which exhibits all of the essential characteristics of a functional transposon, may be involved in driving MIC transposition. We also propose that TALE genes may diversify by fork slippage during the replicative Tn3 family transposition. These mechanisms may play a crucial role in the emergence of Xanthomonas pathogenicity. PMID:25691597

  10. Fayette County Better Buildings Initiative

    SciTech Connect

    Capella, Arthur

    2015-03-04

    The Fayette County Better Buildings Initiative represented a comprehensive and collaborative approach to promoting and implementing energy efficiency improvements. The initiative was designed to focus on implementing energy efficiency improvements in residential units, while simultaneously supporting general marketing of the benefits of implementing energy efficiency measures. The ultimate goal of Fayette Countys Better Buildings Initiative was to implement a total of 1,067 residential energy efficiency retrofits with a minimum 15% estimated energy efficiency savings per unit. Program partners included: United States Department of Energy, Allegheny Power, and Private Industry Council of Westmoreland-Fayette, Fayette County Redevelopment Authority, and various local partners. The program was open to any Fayette County residents who own their home and meet the prequalifying conditions. The level of assistance offered depended upon household income and commitment to undergo a BPI Certified Audit and implement energy efficiency measures, which aimed to result in at least a 15% reduction in energy usage. The initiative was designed to focus on implementing energy efficiency improvements in residential units, while simultaneously supporting general marketing of the benefits of implementing energy efficiency measures. Additionally, the program had components that involved recruitment and training for employment of persons in the energy sector (green jobs), as well as marketing and implementation of a commercial or community facilities component. The residential component of Fayette Countys Better Buildings Initiative involved a comprehensive approach, providing assistance to low- moderate- and market-rate homeowners. The initiative will also coordinate activities with local utility providers to further incentivize energy efficiency improvements among qualifying homeowners. The commercial component of Fayette Countys Better Building Initiative involved grants and loans to assist up to $15,000 projects per commercial structure with a mixture of a grant and financing at 0% for up to three (3) years. The maximum award can be a $5,000 grant and a $10,000 loan. For projects less than $15,000, the award will have a ratio of 1/3 grant and 2/3 loan.

  11. Geothermal development plan: Yuma County

    SciTech Connect

    White, D.H.; Goldstone, L.A.

    1982-08-01

    The Yuma County Area Development Plan evaluated the county-wide market potential for utilizing geothermal energy. The study identified four potential geothermal resource areas with temperatures less than 90/sup 0/C (194/sup 0/F), and in addition, two areas are inferred to contain geothermal resources with intermediate (90/sup 0/C to 150/sup 0/C, 194/sup 0/F to 300/sup 0/F) temperature potential. The resource areas are isolated, although one resource area is located near Yuma, Arizona. One resource site is inferred to contain a hot dry rock resource. Anticipated population growth in the county is expected to be 2 percent per year over the next 40 years. The primary employment sector is agriculture, though some light industry is located in the county. Water supplies are found to be adequate to support future growth without advese affect on agriculture. Six firms were found in Yuma County which may be able to utilize geothermal energy for process heat needs. In addition, several agricultural processors were found, concentrated in citrus processing and livestock raising. Geothermal energy utilization projections suggest that by the year 2000, geothermal energy may economically provide the energy equivalent of 53,000 barrels of oil per year to the industrial sector if developed privately. Geothermal utilization projections increase to 132,000 barrels of oil per year by 2000 if a municipal utility developed the resource.

  12. Origin and structural implications of upper Miocene rhyolites in Kingston Canyon, Piute County, Utah.

    USGS Publications Warehouse

    Rowley, P.D.; Steven, T.A.; Mehnert, H.H.

    1981-01-01

    Kingston Canyon is one of the deepest antecedent canyons in the High Plateaus subprovince of the Colorado Plateaus. Here the E Fork of the Sevier River flows westward transversely across the gently E tilted Sevier Plateau, which is developed on a basin-range fault block uplifted more than 1500m along the Sevier fault zone on the W. Upper Tertiary rhyolites, uncommon in SW Utah, occur both on the northern rim and in the bottom of Kingston Canyon. Those on the northern rim consist of lava flows and volcanic domes of the rhyolite of Forshea Mountain, dated by K/Ar methods at 7.6Ma old. Those in the bottom of Kingston Canyon, the rhyolite of Phonolite Hill, are especially well exposed and provide spectacular examples of a pyroclastic cone whose base is about at river level and a steep-sided volcanic dome emplaced into and through these deposits. The pyroclastic deposits, formerly 500 or more metres thick, consist of airfall, mudflow, and ash-flow(?) material of rhyolite and foreign lithic fragments especially olivine basalt. The dome consists of flow-banded, mostly devitrified rhyolite as much as 500m thick; it has been dated by K/Ar methods at 5.4Ma. In addition to the rhyolites, a dome and lava-flow complex, the rhyodacite of Dry Lake, occurs near the northern rim and is considered to postdate the rhyolite of Forshea Mountain and predate the rhyolite of Phonolite Hill. -from Authors

  13. 76 FR 30152 - East Calloway County Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ...FRL-9310-4] East Calloway County Middle School Mercury Spill Site, Murray, Calloway County...the East Calloway County Middle School Mercury Spill Site located in Murray, Calloway...County [[Page 30153

  14. 75 FR 24944 - Adequacy Status of the Milwaukee-Racine, Door County, Manitowoc County, and Sheboygan County...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-06

    ...In this notice, EPA is notifying the public that we have found that the motor vehicle emissions budgets (MVEBs) for volatile organic compounds (VOCs) and oxides of nitrogen (NOx) in the Milwaukee-Racine area, Door County, Manitowoc County, and Sheboygan County, Wisconsin ozone nonattainment areas are adequate for use in transportation conformity determinations. Wisconsin submitted a......

  15. Executive Summary Multnomah County Drainage District #1

    E-print Network

    US Army Corps of Engineers

    District #1, Peninsula Drainage District #2, Multnomah County Drainage District #1, and Sandy Drainage certification requirements for FEMA accreditation. Any alternative selected to address this deficiency, Multnomah County, Metro, Port of Portland, USACE Portland District, FEMA Region X, Columbia Corridor

  16. The vascular flora of Madison County, Texas

    E-print Network

    Neill, Amanda Kathryn

    2000-01-01

    Vascular plants were collected in Madison County, Texas from 1996-2000 to create a vouchered record of species occurance, abundance, and habitat preference. Plants were collected from 233 sites in the county that are described by thirteen major...

  17. bakerCounty Number of volunteers 43

    E-print Network

    Hill, Jeffrey E.

    industries generate 1,462 jobs (15.2% of total) in Baker County. $48.04 million in revenues. 8 development, North Florida beef and forage Giving Recent donors residing in county 359 FY 2012 donors residing

  18. Spatial variability assessment of soil nutrients in an intense agricultural area, a case study of Rugao County in Yangtze River Delta Region, China

    NASA Astrophysics Data System (ADS)

    Zhao, Yongcun; Xu, Xianghua; Darilek, Jeremy Landon; Huang, Biao; Sun, Weixia; Shi, Xuezheng

    2009-05-01

    Topsoil samples (0-20 cm) ( n = 237) were collected from Rugao County, China. Geostatistical variogram analysis, sequential Gaussian simulation (SGS), and principal component (PC) analysis were applied to assess spatial variability of soil nutrients, identify the possible areas of nutrient deficiency, and explore spatial scale of variability of soil nutrients in the county. High variability of soil nutrient such as soil organic matter (SOM), total nitrogen (TN), available P, K, Fe, Mn, Cu, Zn, and B concentrations were observed. Soil nutrient properties displayed significant differences in their spatial structures, with available Cu having strong spatial dependence, SOM and available P having weak spatial dependence, and other nutrient properties having moderate spatial dependence. The soil nutrient deficiency, defined here as measured nutrient concentrations which do not meet the advisory threshold values specific to the county for dominant crops, namely rice, wheat, and rape seeds, was observed in available K and Zn, and the deficient areas covered 38 and 11%, respectively. The first three PCs of the nine soil nutrient properties explained 62.40% of the total variance. TN and SOM with higher loadings on PC1 are closely related to soil texture derived from different parent materials. The PC2 combined intermediate response variables such as available Zn and P that are likely to be controlled by land use and soil pH. Available B has the highest loading on PC3 and its variability of concentrations may be primarily ascribed to localized anthropogenic influence. The amelioration of soil physical properties (i.e. soil texture) and soil pH may improve the availability of soil nutrients and the sustainability of the agricultural system of Rugao County.

  19. Capability Description for NASA's F/A-18 TN 853 as a Testbed for the Integrated Resilient Aircraft Control Project

    NASA Technical Reports Server (NTRS)

    Hanson, Curt

    2009-01-01

    The NASA F/A-18 tail number (TN) 853 full-scale Integrated Resilient Aircraft Control (IRAC) testbed has been designed with a full array of capabilities in support of the Aviation Safety Program. Highlights of the system's capabilities include: 1) a quad-redundant research flight control system for safely interfacing controls experiments to the aircraft's control surfaces; 2) a dual-redundant airborne research test system for hosting multi-disciplinary state-of-the-art adaptive control experiments; 3) a robust reversionary configuration for recovery from unusual attitudes and configurations; 4) significant research instrumentation, particularly in the area of static loads; 5) extensive facilities for experiment simulation, data logging, real-time monitoring and post-flight analysis capabilities; and 6) significant growth capability in terms of interfaces and processing power.

  20. Identification of Pathogenicity-Related Genes in Biofilm-Defective Acidovorax citrulli by Transposon Tn5 Mutagenesis

    PubMed Central

    Luo, Jinyan; Qiu, Wen; Chen, Lei; Anjum, Syed Ishtiaq; Yu, Menghao; Shan, Changlin; Ilyas, Mehmoona; Li, Bin; Wang, Yanli; Sun, Guochang

    2015-01-01

    Biofilm formation is important for virulence of a large number of plant pathogenic bacteria. Indeed, some virulence genes have been found to be involved in the formation of biofilm in bacterial fruit blotch pathogen Acidovorax citrulli. However, some virulent strains of A. citrulli were unable to format biofilm, indicating the complexity between biofilm formation and virulence. In this study, virulence-related genes were identified in the biofilm-defective strain A1 of A. citrulli by using Tn5 insertion, pathogenicity test, and high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR). Results from this study indicated that 22 out of the obtained 301 mutants significantly decreased the virulence of strain A1 compared to the wild-type. Furthermore, sequence analysis indicated that the obtained 22 mutants were due to the insertion of Tn5 into eight genes, including Aave 4244 (cation diffusion facilitator family transporter), Aave 4286 (hypothetical protein), Aave 4189 (alpha/beta hydrolase fold), Aave 1911 (IMP dehydrogenase/GMP reductase domain), Aave 4383 (bacterial export proteins, family 1), Aave 4256 (Hsp70 protein), Aave 0003 (histidine kinase, DNA gyrase B, and HSP90-like ATPase), and Aave 2428 (pyridoxal-phosphate dependent enzyme). Furthermore, the growth of mutant Aave 2428 was unaffected and even increased by the change in incubation temperature, NaCl concentration and the pH of the LB broth, indicating that this gene may be directly involved in the bacterial virulence. Overall, the determination of the eight pathogenicity-related genes in strain A1 will be helpful to elucidate the pathogenesis of biofilm-defective A. citrulli. PMID:26602922

  1. Annual Performance Evaluation of a Pair of Energy Efficient Houses (WC3 and WC4) in Oak Ridge, TN

    SciTech Connect

    Biswas, Kaushik; Christian, Jeffrey E; Gehl, Anthony C; Jackson, Roderick K; Boudreaux, Philip R

    2012-04-01

    Beginning in 2008, two pairs of energy-saver houses were built at Wolf Creek in Oak Ridge, TN. These houses were designed to maximize energy efficiency using new ultra-high-efficiency components emerging from ORNL s Cooperative Research and Development Agreement (CRADA) partners and others. The first two houses contained 3713 square feet of conditioned area and were designated as WC1 and WC2; the second pair consisted of 2721 square feet conditioned area with crawlspace foundation and they re called WC3 and WC4. This report is focused on the annual energy performance of WC3 and WC4, and how they compare against a previously benchmarked maximum energy efficient house of a similar footprint. WC3 and WC4 are both about 55-60% more efficient than traditional new construction. Each house showcases a different envelope system: WC3 is built with advanced framing featured cellulose insulation partially mixed with phase change materials (PCM); and WC4 house has cladding composed of an exterior insulation and finish system (EIFS). The previously benchmarked house was one of three built at the Campbell Creek subdivision in Knoxville, TN. This house (CC3) was designed as a transformation of a builder house (CC1) with the most advanced energy-efficiency features, including solar electricity and hot water, which market conditions are likely to permit within the 2012 2015 period. The builder house itself was representative of a standard, IECC 2006 code-certified, all-electric house built by the builder to sell around 2005 2008.

  2. Identification of Pathogenicity-Related Genes in Biofilm-Defective Acidovorax citrulli by Transposon Tn5 Mutagenesis.

    PubMed

    Luo, Jinyan; Qiu, Wen; Chen, Lei; Anjum, Syed Ishtiaq; Yu, Menghao; Shan, Changlin; Ilyas, Mehmoona; Li, Bin; Wang, Yanli; Sun, Guochang

    2015-01-01

    Biofilm formation is important for virulence of a large number of plant pathogenic bacteria. Indeed, some virulence genes have been found to be involved in the formation of biofilm in bacterial fruit blotch pathogen Acidovorax citrulli. However, some virulent strains of A. citrulli were unable to format biofilm, indicating the complexity between biofilm formation and virulence. In this study, virulence-related genes were identified in the biofilm-defective strain A1 of A. citrulli by using Tn5 insertion, pathogenicity test, and high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR). Results from this study indicated that 22 out of the obtained 301 mutants significantly decreased the virulence of strain A1 compared to the wild-type. Furthermore, sequence analysis indicated that the obtained 22 mutants were due to the insertion of Tn5 into eight genes, including Aave 4244 (cation diffusion facilitator family transporter), Aave 4286 (hypothetical protein), Aave 4189 (alpha/beta hydrolase fold), Aave 1911 (IMP dehydrogenase/GMP reductase domain), Aave 4383 (bacterial export proteins, family 1), Aave 4256 (Hsp70 protein), Aave 0003 (histidine kinase, DNA gyrase B, and HSP90-like ATPase), and Aave 2428 (pyridoxal-phosphate dependent enzyme). Furthermore, the growth of mutant Aave 2428 was unaffected and even increased by the change in incubation temperature, NaCl concentration and the pH of the LB broth, indicating that this gene may be directly involved in the bacterial virulence. Overall, the determination of the eight pathogenicity-related genes in strain A1 will be helpful to elucidate the pathogenesis of biofilm-defective A. citrulli. PMID:26602922

  3. Archuleta County CO Lineaments

    SciTech Connect

    Zehner, Richard E.

    2012-01-01

    Citation Information: Originator: Earth Science &Observation Center (ESOC), CIRES, University of Colorado at Boulder Originator: Geothermal Development Associates, Reno, Nevada Publication Date: 2012 Title: Archuleta Lineaments Edition: First Publication Information: Publication Place: Reno Nevada Publisher: Geothermal Development Associates, Reno, Nevada Description: This layer traces apparent topographic and air-photo lineaments in the area around Pagosa springs in Archuleta County, Colorado. It was made in order to identify possible fault and fracture systems that might be conduits for geothermal fluids. Geothermal fluids commonly utilize fault and fractures in competent rocks as conduits for fluid flow. Geothermal exploration involves finding areas of high near-surface temperature gradients, along with a suitable plumbing system that can provide the necessary permeability. Geothermal power plants can sometimes be built where temperature and flow rates are high. To do this, georeferenced topographic maps and aerial photographs were utilized in an existing GIS, using ESRI ArcMap 10.0 software. The USA_Topo_Maps and World_Imagery map layers were chosen from the GIS Server at server.arcgisonline.com, using a UTM Zone 13 NAD27 projection. This line shapefile was then constructed over that which appeared to be through-going structural lineaments in both the aerial photographs and topographic layers, taking care to avoid manmade features such as roads, fence lines, and right-of-ways. These lineaments may be displaced somewhat from their actual location, due to such factors as shadow effects with low sun angles in the aerial photographs. Note: This shape file was constructed as an aid to geothermal exploration in preparation for a site visit for field checking. We make no claims as to the existence of the lineaments, their location, orientation, and nature. Spatial Domain: Extent: Top: 4132831.990103 m Left: 311979.997741 m Right: 331678.289280 m Bottom: 4116067.165795 m Contact Information: Contact Organization: Geothermal Development Associates, Reno, Nevada Contact Person: Richard Rick Zehner Address: 3740 Barron Way City: Reno State: NV Postal Code: 89511 Country: USA Contact Telephone: 775-737-7806 Spatial Reference Information: Coordinate System: Universal Transverse Mercator (UTM) WGS1984 Zone 13N False Easting: 500000.00000000 False Northing: 0.00000000 Central Meridian: -105.00000000 Scale Factor: 0.99960000 Latitude of Origin: 0.00000000 Linear Unit: Meter Datum: World Geodetic System 1984 (WGS 1984) Prime Meridian: Greenwich Angular Unit: Degree Digital Form: Format Name: Shape file

  4. Public Health County of Santa Cruz

    E-print Network

    California at Santa Cruz, University of

    Public Health Division County of Santa Cruz HEALTH SERVICES AGENCY POST OFFICE BOX 962, 1080 Date: December 3, 2009 Phone: 831-454-4000 Email: hsadocpio@co.santa-cruz.ca.us County Health Department Announces Flu Vaccine Clinic Santa Cruz County Public Health will hold an additional walk

  5. Community Types and Mortality in Georgia Counties

    ERIC Educational Resources Information Center

    Young, Frank W.

    2012-01-01

    Using an "ecological regional analysis" methodology for defining types of communities and their associated mortality rates, this study of Georgia's 159 counties finds that the suburban and town centered counties have low mortality while the city-centered type predicts low mortality for the whites. The military-centered counties do not predict. The

  6. Trouble Brewing in Orange County. Policy Brief

    ERIC Educational Resources Information Center

    Buck, Stuart

    2010-01-01

    Orange County will soon face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that Orange County faces a total $41.2 billion liability for retiree benefits that are underfunded--including $9.4 billion for the county pension system and an estimated

  7. University of Florida IFAS Extension Orange County

    E-print Network

    Hill, Jeffrey E.

    1 University of Florida IFAS Extension Orange County Bi-annual Report 2013 - 2014 #12;1 #12 Extension Agent in Orange County was Charles Henry Baker, who began work on February 23, 1914. Since then States Department of Agriculture is our Federal partner, the University of Florida and Orange County

  8. Once Distressed, Jackson County Moves On.

    ERIC Educational Resources Information Center

    Baldwin, Fred D.

    1998-01-01

    In 1981, Jackson County, North Carolina, appeared on the first list of "distressed counties" in Appalachia. Since then, the rural county has made significant improvements by investing in its physical infrastructure (which also promotes tourism); fostering economic-development partnerships among governments, small businesses, and local colleges;

  9. 76 FR 13172 - Placer County Water Agency

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-10

    ... Energy Regulatory Commission Placer County Water Agency Notice of Application Tendered for Filing with... Filed: February 23, 2011 d. Applicant: Placer County Water Agency e. Name of Project: Middle Fork... Manager, Placer County Water Agency, 144 Ferguson Road, Auburn, CA 95603; Telephone: (530) 823-4490....

  10. Complete Genome Sequence of Spiroplasma litorale TN-1T (DSM 21781), a Bacterium Isolated from a Green-Eyed Horsefly (Tabanus nigrovittatus)

    PubMed Central

    Lo, Wen-Sui; Lai, Yi-Ching; Lien, Yun-Wei; Wang, Tzu-Haw

    2015-01-01

    Spiroplasma litorale TN-1T (DSM 21781) was isolated from the gut of a green-eyed horsefly (Tabanus nigrovittatus), collected at Ocracoke Island in North Carolina in 1983. Here, we report the complete genome sequence of this bacterium to facilitate the investigation of its biology. PMID:26430038

  11. Directing enhancer-traps and iTol2 end-sequences to deleted BAC ends with loxP- and lox511-Tn10 transposons.

    PubMed

    Chatterjee, Pradeep K

    2015-01-01

    A step-by-step detailed procedure is presented to progressively truncate genomic DNA inserts from either end in BACs. The bacterial transposon Tn10 carrying a loxP or a lox511 site is inserted at random into BAC DNA inside the bacterial cell. The cells are then infected with bacteriophage P1. The Cre protein expressed by phage P1 generates end-deletions by specifically recombining the inserted loxP (or lox511) with the loxP (or lox511) endogenous to and flanking insert DNA in BACs from the respective end. The Cre protein also helps phage P1 transduce the BAC DNA by packaging it in P1 heads. This packaging by P1 not only recovers the rare BAC clones containing Tn10 insertions efficiently but also selects end-truncated BACs from those containing inversions of portions of their DNA caused by transposition of Tn10 in the opposite orientation. The libraries of end-deleted BACs generated by this procedure are suitable for numerous mapping studies. Because DNA in front of the loxP (or lox511) arrowheads in the Tn10 transposon is retained at the newly created BAC end, exogenous DNA cassettes such as enhancer-traps and iTol2 ends can be efficiently introduced into BAC ends for germline expression in zebrafish or mice. The methodology should facilitate functional mapping studies of long-range cis-acting gene regulatory sequences in these organisms. PMID:25239743

  12. ION GNSS+ 2013, Session D1, Nashville, TN, 16-20 September 2013 Page 1/15 Four-constellation Reliability in Challenging

    E-print Network

    Calgary, University of

    ION GNSS+ 2013, Session D1, Nashville, TN, 16-20 September 2013 Page 1/15 Four-constellation Kingdom in 2010. Her research interests include reliability of satellite navigation systems, multi-constellation of ranging signals from new GNSS constellations is expected to result in better system availability

  13. TN1 -Introduzione alla teoria dei numeri -A.A. 2006/2007 Esercitazione in classe in preparazione della I Prova di Valutazione

    E-print Network

    Fontana, Marco

    TN1 - Introduzione alla teoria dei numeri - A.A. 2006/2007 Esercitazione in classe in preparazione: Business Class 9,90 Euro e Economy Class 5,90 Euro. Se il ricavato della vendita dei biglietti ha fruttato (p - 1)((p - 2)!) -1((p - 2)!). Pertanto, moltiplicando ambo i membri per l'inverso aritmetico di -1

  14. nh Gi Tn Hi Ti Nguyn Thin Nhin do Trn Du Deep Horizon D n nng cp ng mn vo Khu

    E-print Network

    tht v dch v gii tr trong vng t thuc B ni V nm bang vng Vnh. Sau s c trn du Deep Horizon, mt lng du v cc hot ng i ph u lm tn tht cc c hi gii tr v lm gim cht lng tri nghim ca du khch n khu

  15. ION GNSS+ 2013, Session D3, Nashville, TN, 16-20 September 2013 Page 1 of 16 A Novel Multipath Estimation and Tracking

    E-print Network

    Calgary, University of

    ION GNSS+ 2013, Session D3, Nashville, TN, 16-20 September 2013 Page 1 of 16 A Novel Multipath the fields of digital signal processing and wireless communications. ABSTRACT It is recognized that multipath remains as a dominant source of ranging error in CDMA and Global Navigation Satellite Systems (GNSS

  16. 77 FR 59573 - Proposed Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ...11-ASO-28] Proposed Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation...Abington, VA. The Tri-Cities Class D airspace description would be amended to...the FAA published a NPRM to amend Class D and Class E airspace, Blountville,...

  17. Library Service in County Jails.

    ERIC Educational Resources Information Center

    Angelides, Cynthia; Berg, Karen

    In order to investigate administrative, financial, and staffing patterns in county jails, the Maine State Library surveyed 45 state library agencies. The specific use of funds and the total number of responses are included in statistical tables. Results from individual states have been categorized, and the survey questionnaire is included. (DS)

  18. Kinship Care in Walton County.

    ERIC Educational Resources Information Center

    Charon, Sara L.; Nackerud, Larry

    1996-01-01

    The quality of life of maltreated children placed with relatives was examined through interviews with nine families in Walton County (Georgia) who had taken in related children. Over half of the children had experienced some improvement in home life, school performance, and their physical and mental status. Kinship care families indicated needs

  19. Ingham County Child Care Centers

    E-print Network

    Feeny, Brian

    Ingham County Child Care Centers Ages Full-Time Part-time Before/AfterSchool Transportation Preschool and Child Development Center 1317 Mason, Dansvilee, MI 517-623-6904 0-17 years Site, East Lansing, MI 517-332-2311 12 months - 5 years EAST LANSING RECREATION AND ARTS CHILD CARE

  20. Truancy in Yolo County, California.

    ERIC Educational Resources Information Center

    Sommer, Barbara; Tyburczy, Jason

    The problem of truancy in the elementary and junior high schools of California's Yolo County was investigated during the 1980-81 academic year by means of questionnaires completed by 16 school principals and interviews with 30 people representing schools, school district offices, law enforcement agencies, the Department of Social Services, and the

  1. Different IncI1 plasmids from Escherichia coli carry ISEcp1-blaCTX-M-15 associated with different Tn2-derived elements.

    PubMed

    Zong, Zhiyong; Ginn, Andrew N; Dobiasova, Hana; Iredell, Jonathan R; Partridge, Sally R

    2015-07-01

    The bla(CTX-M-15) gene, encoding the globally dominant CTX-M-15 extended-spectrum ?-lactamase, has generally been found in a 2.971-kb ISEcp1-bla(CTX-M-15)-orf477? transposition unit, with ISEcp1 providing a promoter. In available IncF plasmid sequences from Escherichia coli, this transposition unit interrupts a truncated copy of transposon Tn2 that lies within larger multiresistance regions. In E. coli, bla(CTX-M-15) is also commonly associated with IncI1 plasmids and here three such plasmids from E. coli clinical isolates from western Sydney 2006-2007 have been sequenced. The plasmid backbones are organised similarly to those of other IncI1 plasmids, but have insertions and/or deletions and sequence differences. Each plasmid also has a different insertion carrying bla(CTX-M-15). pJIE113 (IncI1 sequence type ST31) is almost identical to plasmids isolated from the 2011 E. coli O104:H4 outbreak in Europe, where the typical bla(CTX-M-15) transposition unit interrupts a complete Tn2 inserted directly in the plasmid backbone. In the novel plasmid pJIE139 (ST88), ISEcp1-blaC(TX-M-15)-orf477? lies within a Tn2/3 hybrid transposon. Homologous recombination could explain movement of ISEcp1-bla(CTX-M-15)-orf477? between copies of Tn2 on IncF and IncI1 plasmids and generation of the Tn2/3 hybrid. pJIE174 (ST37) is almost identical to pESBL-12 from the Netherlands and in these plasmids bla(CTX-M-15) is flanked by two copies of IS26 that truncate the transposition unit within a larger region bounded by the ends of Tn2. bla(CTX-M-15) and the associated ISEcp1-derived promoter may be able to move from this structure by the actions of IS26, independently of both ISEcp1 and Tn2. PMID:25929173

  2. Tn5253 family integrative and conjugative elements carrying mef(I) and catQ determinants in Streptococcus pneumoniae and Streptococcus pyogenes.

    PubMed

    Mingoia, Marina; Morici, Eleonora; Morroni, Gianluca; Giovanetti, Eleonora; Del Grosso, Maria; Pantosti, Annalisa; Varaldo, Pietro E

    2014-10-01

    The linkage between the macrolide efflux gene mef(I) and the chloramphenicol inactivation gene catQ was first described in Streptococcus pneumoniae (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in Streptococcus pyogenes (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253 family integrative and conjugative elements (ICEs). The ICE from S. pneumoniae (ICESpn529IQ) was sequenced, whereas the ICEs from S. pyogenes (ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share the intSp 23FST81 integrase gene and an identical Tn916 fragment, whereas ICESpy005IQ has int5252 and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (intSp 23FST81 or int5252). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation, mef(I) and catQ were cotransferred at a high frequency from S. pyogenes Spy005 and at very low frequencies from S. pneumoniae Spn529 and S. pyogenes Spy029. PMID:25070090

  3. Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

    SciTech Connect

    Gunderson, Leonard L.; Moughan, Jennifer; Ajani, Jaffer A.; Pedersen, John E.; Winter, Kathryn A.; Benson, Al B.; Thomas, Charles R.; Haddock, Michael G.; Rich, Tyvin A.; Willett, Christopher G.

    2013-11-15

    Purpose: The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials: DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the logrank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray's test. Results: Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions: TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories.

  4. Analysis of the Shallow Groundwater Flow System at Fire Island National Seashore, Suffolk County, New York

    USGS Publications Warehouse

    Schubert, Christopher E.

    2010-01-01

    Fire Island National Seashore (FIIS) occupies 42 kilometers of the barrier island for which it is named that lies off the southern shore of Suffolk County, N.Y. Freshwater in the highly permeable, sandy aquifer underlying Fire Island is bounded laterally by marine surface waters and at depth by saline groundwater. Interspersed throughout FIIS are 17 pre-existing residential communities that in summer months greatly increase in population through the arrival of summer residents and vacationers; in addition, the National Park Service (NPS) has established several facilities on the island to accommodate visitors to FIIS. The 2.2 million people estimated by the NPS to visit Fire Island annually impact groundwater quality through the release of waste-derived contaminants, such as nutrients, pathogens, and organic compounds, into the environment. Waste-contaminated groundwater can move through the aquifer and threaten the ecological health of the adjacent back-barrier estuaries to which much of the groundwater ultimately discharges. In 2004, the U.S. Geological Survey (USGS), in cooperation with the NPS, began a 3-year investigation to (1) collect groundwater levels and water-quality (nutrient) samples, (2) develop a three-dimensional model of the shallow (water-table) aquifer system and adjacent marine surface waters, and (3) calculate nitrogen loads in simulated groundwater discharges from the aquifer to back-barrier estuaries and the ocean. The hydrogeology of the shallow aquifer system was characterized from the results of exploratory drilling, geophysical surveying, water-level monitoring, and water-quality sampling. The investigation focused on four areas-the communities of Kismet and Robbins Rest, the NPS Visitor Center at Watch Hill, and the undeveloped Otis Pike Fire Island High Dune Wilderness. Thirty-five observation wells were installed within FIIS to characterize subsurface hydrogeology and establish a water-table monitoring network in the four study areas. A variable-density model of the shallow aquifer system and adjacent marine surface waters was developed to simulate groundwater flow patterns and rates. Nitrogen loads from the shallow aquifer system were calculated from representative total nitrogen (TN) concentrations and simulated groundwater discharges to back-barrier estuaries and the ocean. The model simulates groundwater directions, velocities, and discharge rates under 2005 mean annual conditions. Groundwater budgets were developed for recharge areas of similar land use that contribute freshwater to back-barrier estuaries, the ocean, and subsea-discharge zones. Total freshwater discharge from the shallow aquifer system is about 43,500 cubic meters per day (m3/d) (79.8 percent) to back-barrier estuaries and about 10,200 m3/d (18.7 percent) to the ocean; about 836 m3/d (1.5 percent) may exit the system as subsea underflow. The total contribution of fresh groundwater to shoreline discharge zones amounts to about 53,700 m3/d (98.5 percent). The median age of freshwater discharged to back-barrier estuaries and the ocean was 3.4 years, and the 95th-percentile age was 20 years. The TN concentrations and loads under 2005 mean annual conditions for areas that contribute fresh groundwater to back-barrier estuaries and the ocean were calculated for the principal land uses on Fire Island. The overall TN load from the shallow aquifer system to shoreline discharge zones is about 16,200 kilograms per year (kg/yr) (82.2 percent) to back-barrier estuaries and about 3,500 kg/yr (17.8 percent) to the ocean. The overall TN load to marine surface waters amounts to about 19,700 kg/yr-roughly 6 percent of the annual TN load from shallow groundwater entering the South Shore Estuary Reserve (SSER) from the Suffolk County mainland, which is about 345,000 kg/yr. In contrast to the TN load from shallow groundwater for the SSER watershed, which annually yields about 353 kilograms per square kilometer (kg/km2), the overall TN loa

  5. Coherence and Variability of Ground Motion Over 600 m of the Nonvolcanic Tremor Array Site at Mooring, TN

    NASA Astrophysics Data System (ADS)

    Huda, M. M.; Langston, C. A.

    2014-12-01

    This study examines wave coherency for high frequency P and S waves in the New Madrid Seismic Zone at a site near Mooring, TN. An L-shaped array consisting of 19-seismometers and having arm lengths of 600m located on Holocene fluvial sediments of the Mississippi River was used to examine wave coherency appropriate for many sites throughout the Mississippi embayment and other sediment sites associate with large rivers. Data from local and regional earthquakes within a distance range of 300km show that coherency within the frequency band of 0.5 to 16 Hz degrades with interstation distance across the array according to an empirical exponential model. Vertical component P-waves are coherent over nearly 2 horizontal wavelengths. However, the coherency for horizontal component S-waves degrades more rapidly than the vertical component P waves. S-waves become significantly incoherent at distances of only 0.2 horizontal wavelength and become completely incoherent after 0.5 wavelength. The results of this study show clear and quick decay of wave coherence likely to occur in strong ground motions from nearby earthquakes. The observed incoherence can be a significant factor for the response of structures having foundation lengths of even 100m for a nearby large magnitude earthquake in New Madrid Seismic Zone.

  6. Characterization of transposon Tn1528, which confers amikacin resistance by synthesis of aminoglycoside 3'-O-phosphotransferase type VI.

    PubMed

    Lambert, T; Gerbaud, G; Courvalin, P

    1994-04-01

    Providencia stuartii BM2667, which was isolated from an abdominal abscess, was resistant to amikacin by synthesis of aminoglycoside 3'-O-phosphotransferase type VI. The corresponding gene, aph(3')-VIa, was carried by a 30-kb self-transferable plasmid of incompatibility group IncN. The resistance gene was cloned into pUC18, and the recombinant plasmid, pAT246, was transformed into Escherichia coli DH1 (recA) harboring pOX38Gm. The resulting clones were mixed with E. coli HB101 (recA), and transconjugants were used to transfer pAT246 by plasmid conduction to E. coli K802N (rec+). Analysis of plasmid DNAs from the transconjugants of K802N by agarose gel electrophoresis and Southern hybridization indicated the presence of a transposon, designated Tn1528, in various sites of pOX38Gm. This 5.2-kb composite element consisted of aph(3')-VIa flanked by two direct copies of IS15-delta and transposed at a frequency of 4 x 10(-5). It therefore appears that IS15-delta, an insertion sequence widely spread in gram-negative bacteria, is likely responsible for dissemination to members of the family Enterobacteriaceae of aph(3')-VIa, a gene previously confined to Acinetobacter spp. PMID:8031033

  7. Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif

    SciTech Connect

    Klenchin, Vadim A.; Czyz, Agata; Goryshin, Igor Y.; Gradman, Richard; Lovell, Scott; Rayment, Ivan; Reznikoff, William S.

    2009-01-15

    Bacterial DNA transposition is an important model system for studying DNA recombination events such as HIV-1 DNA integration and RAG-1-mediated V(D)J recombination. This communication focuses on the role of protein-phosphate contacts in manipulating DNA structure as a requirement for transposition catalysis. In particular, the participation of the nontransferred strand (NTS) 5' phosphate in Tn5 transposition strand transfer is analyzed. The 5' phosphate plays no direct catalytic role, nonetheless its presence stimulates strand transfer {approx}30-fold. X-ray crystallography indicates that transposase-DNA complexes formed with NTS 5' phosphorylated DNA have two properties that contrast with structures formed with complexes lacking the 5' phosphate or complexes generated from in-crystal hairpin cleavage. Transposase residues R210, Y319 and R322 of the (R)YREK motif coordinate the 5' phosphate rather than the subterminal NTS phosphate, and the 5' NTS end is moved away from the 3' transferred strand end. Mutation R210A impairs the 5' phosphate stimulation. It is posited that DNA phosphate coordination by R210, Y319 and R322 results in movement of the 5' NTS DNA away from the 3'-end thus allowing efficient target DNA binding. It is likely that this role for the newly identified RYR triad is utilized by other transposase-related proteins.

  8. Transportation of a 451 ton generator stator and a 234 ton generator rotor from Hartsville, TN, to Los Alamos, NM

    SciTech Connect

    Boenig, H.J.; Rogers, J.D.; McLelland, G.R.; Pelts, C.T.; McLelland Engineering, Dallas, TX; Reliance Crane and Rigging, Inc., Phoenix, AZ )

    1989-01-01

    A 1430 MVA steam turbine generator was acquired from a cancelled nuclear power plant in Tennessee to be used as the pulsed power and energy storage unit for the Confinement Physics Research Facility being built at Los Alamos, NM. The transportation from Hartsville, near Nashville, TN, to Los Alamos, NM, of the two largest single pieces of the generator, a 451 t stator and a 234 t rotor presented a special challenge. Details of the move, by barge from Hartsville to Catoosa, near Tulsa, OK, by rail from Catoosa to Lamy, near Santa Fe, NM, and by road from Lamy to Los Alamos are described. The greatest difficulty of the successful move was the crossing of the Rio Grande river on an existing reinforced concrete bridge. The two-lane wide road transporters for the stator and rotor were fitted with outriggers to provide a four-lane wide vehicle, thus spreading the load over the entire bridge width and meeting acceptable load distribution and bridge safety factors. 2 refs., 6 figs.

  9. Evaluation of analytical performance and comparison of clinical results of the new generation method AccuTnI+3 for the measurement of cardiac troponin I using both patients and quality control plasma samples.

    PubMed

    Storti, Simona; Masotti, Silvia; Prontera, Concetta; Franzini, Maria; Buzzi, Paola; Casagranda, Ivo; Ciofini, Enrica; Zucchelli, Gian Carlo; Ndreu, Rudina; Passino, Claudio; Clerico, Aldo

    2015-12-01

    The study aims are to evaluate the analytical performance and the clinical results of the chemiluminescent Access AccuTnI+3 immunoassay for the determination of cardiac troponin I (cTnI) with DxI 800 and Access2 platforms and to compare the clinical results obtained with this method with those of three cTnI immunoassays, recently introduced in the European market. The limits of blank (LoB), detection (LoD), and quantitation (LoQ) at 20% CV and 10% CV were 4.5ng/L and 10.9ng/L, 17.1 and 30.4ng/L, respectively. The results of STAT Architect high Sensitive TnI (Abbott Diagnostics), ADVIA Centaur Troponin I Ultra (Siemens Healthcare Diagnostics), ST AIA-Pack cTnI third generation (Tosoh Bioscience), and Access AccuTnI+3 (Beckman Coulter Diagnostics) showed very close correlations (R ranging from 0.901 to 0.994) in 122 samples of patients admitted to the emergency department. However, on average there was a difference up to 2.4-fold between the method measuring the highest (ADVIA method) and lowest cTnI values (AccuTnI+3 method). The consensus mean values between methods ranged from 6.2% to 29.6% in 18 quality control samples distributed in an external quality control study (cTnI concentrations ranging from 29.3ng/L to 1557.5ng/L). In conclusion, the results of our analytical evaluation concerning the AccuTnI+3 method, using the DxI platform, are well in agreement with those suggested by the manufacturer as well as those reported by some recent studies using the Access2 platform. Our results confirm that the AccuTnI+3 method for the Access2 and DxI 800 platforms is a clinically usable method for cTnI measurement. PMID:26391123

  10. Budgeting in Texas Counties, 1931-1940.

    E-print Network

    Bradshaw, H. C.

    1941-01-01

    ( ,QGRICIJLTURAIJ AND MECHANICAL COLLEGE OF T'EXAS T. 0. WALTON, President [Blank Page in Original Bulletin] The uniform budget law applying to Texas counties was passed in 1931. The law requires that each county, wieh the exception of Harris County which... is governed by a special statute, prepare a budget mually. A copy of the budget must be filed with the county clerk, and a second copy must be filed with the state comptroller of public accounts. Judging by the number of budgets filed with the state...

  11. County Extension Home Economics Program: Organizational Relationships.

    E-print Network

    Cline, June

    1982-01-01

    county residents determine tbdr grams to answep th agent@work with a lanned, logid, or the best results with *he most people. The Extension home economics program seeks to improve the quality af home and h i l y life, To do this, sion agents (home... treasurers dh&y to theLooWty Tern Extensioh Homemakers Association ch8inna~r. The county Texas ExtessiQJl Ua~qemakers Asmciatiw chairman attends county Extension h0menialrei-s council meetings regularly. The county chairman should be able to disass any...

  12. 75 FR 25308 - Environmental Impact Statement: Winnebago County, IL and Rock County, WI

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF TRANSPORTATION Federal Highway Administration Environmental Impact Statement: Winnebago County, IL and Rock County, WI... Beloit, Rock County, Wisconsin to the interchange of Rockton Road and I-90 southeast of South...

  13. 75 FR 26709 - Clarke County Water Supply Project, Clarke County, IA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... Natural Resources Conservation Service Clarke County Water Supply Project, Clarke County, IA AGENCY... Water Supply Project, Clarke County, Iowa. ] FOR FURTHER INFORMATION CONTACT: Richard Sims, State... comments by NRCS information related to water supply demand requirements for permitting by the State...

  14. The Economic Impact of Schenectady County Community College on Schenectady County, 1981-82.

    ERIC Educational Resources Information Center

    Chestnut, Erma Ruth

    This report on the economic impact of Schenectady County Community College (SCCC) uses a modification of the Caffrey and Isaacs model to assess SCCC-related local business volume, SCCC costs and benefits to the Schenectady County government, and the likely impact on the county if SCCC did not exist. Part I provides background to the study,

  15. Neutron diffraction study of the nuclear and magnetic structure of the quasi-one-dimensional compound CuSiO3 around TN=8 K

    NASA Astrophysics Data System (ADS)

    Wolfram, H.; Otto, H. H.; Cwik, M.; Braden, M.; Andr, G.; Boure, F.; Baenitz, M.; Steglich, F.

    2004-04-01

    We investigated the nuclear and magnetic structure of CuSiO3 by neutron powder diffraction methods at room temperature and around TN=8 K. With decreasing temperature the lattice parameters showed, similar to CuGeO3, decreasing a and b parameters and a slightly increasing c parameter down the chain direction. Below the second order phase transition at TN we observed for CuSiO3 the appearance of a small set of magnetic superstructure reflections; the determined antiferromagnetic order can be described by a commensurate propagation vector of q=(1/2, 0, 1/8) with respect to the chemical Pbmm unit cell. From the long modulation along the chain a ferromagnetic next neighbor magnetic exchange is deduced, whereas next nearest neighbor coupling is expected to be antiferromagnetic.

  16. Characterization of Tn3000, a Transposon Responsible for blaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India.

    PubMed

    Campos, Juliana Coutinho; da Silva, Maria Jos Flix; Dos Santos, Paulo Roberto Nascimento; Barros, Elaine Menezes; Pereira, Mayne de Oliveira; Seco, Bruna Mara Silva; Magagnin, Cibele Massotti; Leiroz, Leonardo Kalab; de Oliveira, Tho Gremen Mimary; Faria-Jnior, Clio de; Cerdeira, Louise Teixeira; Barth, Afonso Lus; Sampaio, Suely Carlos Ferreira; Zavascki, Alexandre Prehn; Poirel, Laurent; Sampaio, Jorge Luiz Mello

    2015-12-01

    In Enterobacteriaceae, the blaNDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the blaNDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFIIK and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the blaNDM-1 gene may be transmitted by Tn3000 in different parts of the world. PMID:26392506

  17. 77 FR 49779 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-17

    ...DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee...SUMMARY: The Big Horn County Resource Advisory Committee...ADDRESSES: The meeting will be held at the Big Horn County Weed and Pest Building,...

  18. 75 FR 49886 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-16

    ...DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee...SUMMARY: The Big Horn County Resource Advisory Committee...ADDRESSES: The meeting will be held at the Big Horn County Courthouse, Commissioners...

  19. 76 FR 26240 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-06

    ...DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee...SUMMARY: The Big Horn County Resource Advisory Committee...ADDRESSES: The meeting will be held at the Big Horn County Weed and Pest Building,...

  20. 78 FR 33326 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-04

    ...DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee...SUMMARY: The Big Horn County Resource Advisory Committee...ADDRESSES: The meeting will be held at Big Horn County Weed and Pest Building,...

  1. 75 FR 71069 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ...DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee...SUMMARY: The Big Horn County Resource Advisory Committee...ADDRESSES: The meeting will be held at the Big Horn County Weed and Pest Building,...

  2. 76 FR 47141 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-04

    ...DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee...SUMMARY: The Big Horn County Resource Advisory Committee...ADDRESSES: The meeting will be held at the Big Horn County Weed and Pest Building,...

  3. Silver City Grant County Airport (SVC) Pavement Condition and Analysis

    E-print Network

    Cal, Mark P.

    Silver City Grant County Airport (SVC) Pavement Condition and Analysis Submitted to: Jane M. Lucero ....................................1. Airport Conditions at Silver City Grant County Airport (SVC) 4 ..................................Figure 1. Geographic Location of Silver City Grant County Airport (SVC) 4 ......................Table 1

  4. 6. Photocopy of illustration on Map of Chester County Pennsylvania, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Photocopy of illustration on Map of Chester County Pennsylvania, by T.J. Kennedy, published by R.L. Barnes, Philadelphia, Pennsylvania, 1856 SOUTHWEST CORNER, 1856 - Bank of Chester County, 17 North High Street, West Chester, Chester County, PA

  5. NORTHERN SAN DIEGO COUNTY LAGOONS Historical Ecology Investigation

    E-print Network

    NORTHERN SAN DIEGO COUNTY LAGOONS Historical Ecology Investigation REGIONAL PATTERNS, LOCAL California State University at Northridge NORTHERN SAN DIEGO COUNTY LAGOONS Historical Ecology Investigation, Longcore TR, Stein ED, Dark SJ, Dusterhoff SR. 2014. Northern San Diego County Lagoons Historical Ecology

  6. 75 FR 54419 - Environmental Impact Statement: Yellowstone County, MT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-07

    ... Federal Highway Administration Environmental Impact Statement: Yellowstone County, MT AGENCY: Federal... highway project in Yellowstone County, Montana. FOR FURTHER INFORMATION CONTACT: Brian Hasselbach, Right... (I-90) and Old Highway 312 in or near the city of Billings, Yellowstone County, Montana....

  7. Digital computer processing of LANDSAT data for North Alabama. [Linestone County, Madison County, Jackson County, Marshall County, and DeKalb County

    NASA Technical Reports Server (NTRS)

    Bond, A. D.; Atkinson, R. J.; Lybanon, M.; Ramapriyan, H. K.

    1977-01-01

    Computer processing procedures and programs applied to Multispectral Scanner data from LANDSAT are described. The output product produced is a level 1 land use map in conformance with a Universal Transverse Mercator projection. The region studied was a five-county area in north Alabama.

  8. Genetic Characterization of a Tn5-Disrupted Glycosyltransferase Gene Homolog in Brucella abortus and Its Effect on Lipopolysaccharide Composition and Virulence

    PubMed Central

    McQuiston, J. R.; Vemulapalli, R.; Inzana, T. J.; Schurig, G. G.; Sriranganathan, N.; Fritzinger, D.; Hadfield, T. L.; Warren, R. A.; Snellings, N.; Hoover, D.; Halling, S. M.; Boyle, S. M.

    1999-01-01

    We constructed a rough mutant of Brucella abortus 2308 by transposon (Tn5) mutagenesis. Neither whole cells nor extracted lipopolysaccharide (LPS) from this mutant, designated RA1, reacted with a Brucella O-side-chain-specific monoclonal antibody (MAb), Bru-38, indicating the absence of O-side-chain synthesis. Compositional analyses of LPS from strain RA1 showed reduced levels of quinovosamine and mannose relative to the levels in the parental, wild-type strain, 2308. We isolated DNA flanking the Tn5 insertion in strain RA1 by cloning a 25-kb XbaI genomic fragment into pGEM-3Z to create plasmid pJM6. Allelic exchange of genomic DNA in B. abortus 2308 mediated by electroporation of pJM6 produced kanamycin-resistant clones that were not reactive with MAb Bru-38. Southern blot analysis of genomic DNA from these rough clones revealed Tn5 in a 25-kb XbaI genomic fragment. A homology search with the deduced amino acid sequence of the open reading frame disrupted by Tn5 revealed limited homology with various glycosyltransferases. This B. abortus gene has been named wboA. Transformation of strain RA1 with a broad-host-range plasmid bearing the wild-type B. abortus wboA gene resulted in the restoration of O-side-chain synthesis and the smooth phenotype. B. abortus RA1 was attenuated for survival in mice. However, strain RA1 persisted in mice spleens for a longer time than the B. abortus vaccine strain RB51, but as expected, neither strain induced antibodies specific for the O side chain. PMID:10417145

  9. Dissemination of blaOXA-23 in Acinetobacter spp. in China: main roles of conjugative plasmid pAZJ221 and transposon Tn2009.

    PubMed

    Liu, Li-Lin; Ji, Shu-Juan; Ruan, Zhi; Fu, Ying; Fu, Yi-Qi; Wang, Yan-Fei; Yu, Yun-Song

    2015-04-01

    Production of the OXA-23 carbapenemase is the most common reason for the increasing carbapenem resistance in Acinetobacter spp. This study was conducted to reveal the genetic basis of blaOXA-23 dissemination in Acinetobacter spp. in China. A total of 63 carbapenem-resistant OXA-23-producing Acinetobacter sp. isolates, representing different backgrounds, were selected from 28 hospitals in 18 provinces for this study. Generally, two patterns of plasmids carrying blaOXA-23 were detected according to S1-nuclease pulsed-field gel electrophoresis and Southern blot hybridization. A ca. 78-kb plasmid, designated pAZJ221, was found in 23 Acinetobacter baumannii and three Acinetobacter nosocomialis isolates, while a novel ca. 50-kb plasmid was carried by only two other A. baumannii isolates. Three of these isolates had an additional copy of blaOXA-23 on the chromosome. Transformation of the two plasmids succeeded, but only pAZJ221 was conjugative. Plasmid pAZJ221 was sequenced completely and found to carry no previously known resistance genes except blaOXA-23. The blaOXA-23 gene of the remaining 35 isolates was chromosome borne. The blaOXA-23 genetic environments were correlated with Tn2009 in 57 isolates, Tn2008 in 5 isolates, and Tn2006 in 1 isolate. The MIC values for the carbapenems with these isolates were not significantly associated with the genomic locations or the copy numbers of blaOXA-23. Overall, these observations suggest that the plasmid pAZJ221 and Tn2009 have effectively contributed to the wide dissemination of blaOXA-23 in Acinetobacter spp. in China and that horizontal gene transfer may play an important role in dissemination of the blaOXA-23 gene. PMID:25605357

  10. The Math of Space A space is a set of points, typically characterized as a tuple (t1, t2, . . . , tn) in terms of

    E-print Network

    Dahlquist, Kam D.

    The Math of Space 1 Overview · A space is a set of points, typically characterized as a tuple (t1, t2, . . . , tn) in terms of that space. We frequently speak about objects in space; such an object in a space S, can be viewed as a subset of points X such that X S. · Some examples of spaces are: ­ R2

  11. Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene.

    PubMed Central

    DeShazer, D; Brett, P J; Carlyon, R; Woods, D E

    1997-01-01

    Burkholderia pseudomallei is a human and animal pathogen in tropical regions, especially Southeast Asia and northern Australia. Currently little is known about the genetics and molecular biology of this organism. In this report, we describe the mutagenesis of B. pseudomallei with the transposon Tn5-OT182. B. pseudomallei 1026b transposon mutants were obtained at a frequency of 4.6 x 10(-4) per initial donor cell, and the transposon inserted randomly into the chromosome. We used Tn5-OT182 to identify the flagellin structural gene, fliC. We screened 3,500 transposon mutants and identified 28 motility mutants. Tn5-OT182 integrated into 19 unique genetic loci encoding proteins with homology to Escherichia coli and Salmonella typhimurium flagellar and chemotaxis proteins. Two mutants, MM35 and MM36, contained Tn5-OT182 integrations in fliC. We cloned and sequenced fliC and used it to complement MM35 and MM36 in trans. The fliC transcriptional start site and a sigmaF-like promoter were identified by primer extension analysis. We observed a significant difference in the expression of two distinct fliC-lacZ transcriptional fusions during bacterial growth, suggesting the presence of a latent intragenic transcriptional terminator in fliC. There was no significant difference in the virulence of 1026b compared to that of MM36 in diabetic rats or Syrian hamsters, suggesting that flagella and/or motility are probably not virulence determinants in these animal models of B. pseudomallei infection. A phylogenetic analysis based on the flagellins from a variety of bacterial species supported the recent transfer of B. pseudomallei from the genus Pseudomonas to Burkholderia. PMID:9079894

  12. Complete characterisation of Tn5530 from Burkholderia cepacia strain 2a (pIJB1) and studies of 2,4-dichlorophenoxyacetate uptake by the organism.

    PubMed

    Poh, R P-C; Smith, A R W; Bruce, I J

    2002-07-01

    The complete genetic characterisation of Tn5530 in Burkholderia cepacia strain 2a (pIJB1) has been accomplished, indicating that it is a Tn3-like transposon with a complex structure bearing operons for the catabolism of 2,4-dichlorophenoxyacetate (2,4-D) and malonate. Tn5530 is terminated at both ends by the IS1071::IS1471 element and the 2,4-D- and malonate-dissimilatory operons are separated by a region encoding a putA and lrp gene and a gene encoding a chloride channel protein. The chloride channel protein may have a role in the expulsion of chloride ions liberated by the dissimilation of 2,4-D. In addition, a putative transposase with a high level of sequence similarity to those of plasmid pGH1 from Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. glycinea, and a transcription factor similar to those of the TetR family with low but significant levels of sequence similarity to those identified in a number of other organisms was observed. The entire Tn5530 sequence length, including the IS1071::IS1471 elements, was found to be 40,956bp, and pIJB1 was replicon-typed and otherwise characterised as being of the IncP-1beta subgroup, bearing merA and merD genes conferring resistance to mercuric chloride. The rate of uptake of 2,4-D by B. cepacia strain 2a was observed to proceed more readily at acid pH, suggesting involvement of the undissociated form of the compound. Uptake did not show saturation kinetics, was concentration-dependent, and appeared to occur in two stages; an initial accumulation followed by a linear second phase. Uptake could be inhibited by sodium azide but not by arsenate, N,N(')-dicyclohexylcarbodi-imide (DCCD) or carbonylcyanide m-chlorophenyl-hydrazone (CCCP) suggesting that it is not energy-dependent. PMID:12206751

  13. nh gi Tn hi Ti nguyn Thin nhin do Trn du Deep Horizon Khi phc loi chim ng bin ti vng b bin Alabama

    E-print Network

    nh gi Tn hi Ti nguyn Thin nhin do Trn du Deep Horizon Khi phc loi chim ng bin ti vng b Alabama s hnh thnh 5 nn lm t ca chim ng dc theo vng b bin Mobile v Baldwin, Alabama tng c hi lm c th c tm thy quanh nm ti khu vc b bin Alabama. Chim ng bin i hi nhng khu vc lm t rng ri vi

  14. Efficiency and bacterial populations related to pollutant removal in an upflow microaerobic sludge reactor treating manure-free piggery wastewater with low COD/TN ratio.

    PubMed

    Meng, Jia; Li, Jiuling; Li, Jianzheng; Sun, Kai; Antwi, Philip; Deng, Kaiwen; Wang, Cheng; Buelna, Gerardo

    2016-02-01

    A novel upflow microaerobic sludge reactor (UMSR) had proved excellent in nitrogen removal from manure-free piggery wastewater characterized by high concentration of ammonium (NH4(+)-N) and low chemical oxygen demand (COD) to total nitrogen (TN) ratio, but the biological mechanism in the UMSR was still indeterminate. With a constant nitrogen loading rate of 1.10kg/(m(3)d) at hydraulic retention time 8h, the UMSR was kept performing for 67days in the present research and the average load removal of COD, NH4(+)-N and TN was as high as 0.72, 0.76 and 0.94kg/(m(3)d), respectively. Compared with the inoculated sludge, the acclimated sludge was richer in genera responsible for the biological removal of carbon, nitrogen and phosphorus. Ammonium oxidation bacteria, heterotrophic denitrifiers, autotrophic denitrifiers and phosphate accumulating organisms coexisted perfectly in the microaerobic system, and their synergistic action made the UMSR perform well in COD, NH4(+)-N, TN and phosphate removal. PMID:26649897

  15. 76 FR 19030 - Lyon & Mineral County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ...DEPARTMENT OF AGRICULTURE Forest Service Lyon & Mineral County Resource Advisory Committee AGENCY: Forest Service...SUMMARY: The Lyon and Mineral County Resource Advisory Committee will meet in Smith...

  16. Symbiotic Streptomyces sp. TN119 GH 11 xylanase: a new pH-stable, protease- and SDS-resistant xylanase.

    PubMed

    Zhou, Junpei; Shi, Pengjun; Zhang, Rui; Huang, Huoqing; Meng, Kun; Yang, Peilong; Yao, Bin

    2011-04-01

    A pH-stable and protease-resistant xylanase (XynB119) was identified from Streptomyces sp. TN119, a strain isolated from the gut luminal contents of longhorned beetle (Batocera horsfieldi) larvae. Using the GC TAIL-PCR method, the 1,026-bp coding gene (xynB119) with 67.3% GC content was successfully cloned and expressed in Escherichia coli. It encodes a 341-residue polypeptide with a calculated molecular mass of 35.9 kDa, including a putative 41-residue signal peptide, a catalytic domain of glycosyl hydrolase (GH) family 11, a short Gly/Pro-rich linker, and a family 2 cellulose-binding domain (CBM 2). The deduced amino acid sequence is most similar to (61.9% identity) an endo-1,4-?-xylanase from Streptomyces thermoviolaceus OPC-520. Purified recombinant XynB119 exhibited peak activity at 50 C and pH 7.0, remained stable over a broad pH range (retaining >70% activity after incubation at pH 1.0-11.0 for 1 h at 37 C without substrate), had strong protease resistance (retaining >90% activity after proteolytic treatment at 37 C for 1 h) and SDS resistance (at 100 mM). These properties make XynB119 promising for application in the feed industry and valuable for basic research. Compared to r-XynB119, the r-XynB119 derivative without CBM 2 and linker region (r-XynB119d) exhibited a decreased pH stability of >25% at extreme pHs (pH 1.0-3.0 and pH 11.0-12.0). PMID:20686913

  17. Impacts of cool cities on air quality: A preliminary modeling assessment for Nashville TN, Dallas TX and Atlanta GA

    SciTech Connect

    Taha, Haider

    1998-06-15

    Previous atmospheric modeling efforts that concentrated on the Los Angeles Basin suggested beneficial and significant air quality impacts from cool cities strategies. This paper discusses an extension of similar modeling efforts to three regions, Atlanta GA, Dallas - Ft. Worth TX, and Nashville TN, that experience smog and air quality problems. According to the older ozone air quality standard (120 ppb), these regions were classified as serious, moderate, and marginal, respectively, but may be out of compliance with respect to the newer, 80-ppb/8-hours standard. Results from this exploratory modeling work suggest a range of possible impacts on meteorological and air quality conditions. For example, peak ozone concentrations during each region's respective episode could be decreased by 1-6 ppb (conservative and optimistic scenarios, respectively) in Nashville, 5-15 ppb in Dallas - Fort Worth, and 5-12 ppb in Atlanta following implementation of cool cities. The reductions are generally smaller than those obtained from simulating the Los Angeles Basin but are still significant. In all regions, the simulations suggest, the net, domain-wide effects of cool cities are reductions in ozone mass and improvements in air quality. In Atlanta, Nashville, and Dallas, urban areas benefiting from reduced smog reach up to 8460, 7350, and 12870 km{sup 2} in area, respectively. Results presented in this paper should be taken as exploratory and preliminary. These will most likely change during a more comprehensive modeling study to be started soon with the support of the US Environmental Protection Agency. The main purpose of the present project was to obtain the initial data (emission inventories) for these regions, simulate meteorological conditions, and perform preliminary sensitivity analysis. In the future, additional regions will be simulated to assess the potential of cool cities in improving urban air quality.

  18. Geothermal development plan: Pinal county

    SciTech Connect

    White, D.H.

    1981-01-01

    Wells drilled in the county provide evidence of geothermal energy sufficient for process heat and space heating and cooling applications. Annual energy consumption was estimated for industries whose process heat requirements are less than 105/sup 0/C (221/sup 0/F). This information was then used to model the introduction of geothermal energy into the process heat market. Also, agriculture and agribusiness industries were identified. Many of these are located on or near a geothermal resource and might be able to utilize geothermal energy in their operations.

  19. TN International and ITS operational feedback regarding the decommissioning of obsolete casks dedicated to the transport and/or storage of nuclear raw materials, fuel and used fuel

    SciTech Connect

    Blachet, L.; Bimet, F.; Rennesson, N.

    2008-07-01

    Within the AREVA group, TN International is a major actor regarding the design of casks and transportation for the nuclear cycle. In the early 2005, TN International has started the project of decommissioning some of its own equipment and was hence the first company ever in the AREVA Group to implement this new approach. In order to do so, TN International has based this project by taking into account the AREVA Sustainable Development Charter, the French regulatory framework, the ANDRA (Agence Nationale pour la Gestion des Dechets Radioactifs - National Agency for the radioactive waste management) requirements and has deployed a step by step methodology such as radiological characterization following a logical route. The aim was to define a standardized process with optimized solutions regarding the diversity of the cask's fleet. As a general matter, decommissioning of nuclear casks is a brand new field as the nuclear field is more familiar with the dismantling of nuclear facilities and/or nuclear power plant. Nevertheless existing workshops, maintenance facilities, measurements equipments and techniques have been exploited and adapted by TN International in order to turn an ambitious project into a permanent and cost-effective activity. The decommissioning of the nuclear casks implemented by TN International regarding its own needs and the French regulatory framework is formalized by several processes and is materialized for instance by the final disposal of casks as they are or in ISO container packed with cut-off casks and big bags filled with crushed internal cask equipments, etc. The first part of this paper aims to describe the history of the project that started with a specific environmental analysis which took into account the values of AREVA as regards the Sustainable Development principles that were at the time and are still a topic of current concern in the world. The second part will deal with the definition, the design and the implementation of the decommissioning processes and the applied techniques. The third part will present a two years operational feedback. The last part will introduce new processes which are currently under investigation and will put into light that decommissioning of nuclear casks is a continuous activity that is in perpetual mutation. (authors)

  20. A Rural County's Response to Homelessness.

    ERIC Educational Resources Information Center

    Loughran, Elizabeth Lee; White, Priscilla

    This paper describes the response of one locality, a rural county in Western Massachusetts, to the reality of rural homelessness. Jessie's House, in Hampshire County, Massachusetts, is a short-term emergency shelter providing meals, housing, and advocacy to homeless families and individuals. The shelter has a staff of three full-time residents and

  1. Educational and Demographic Profile: Lake County.

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Lake County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced communication

  2. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... activities, specifically urban renewal and publicly assisted housing. In determining whether a county has the... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development...

  3. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... activities, specifically urban renewal and publicly assisted housing. In determining whether a county has the... 24 Housing and Urban Development 3 2012-04-01 2012-04-01 false Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development...

  4. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... activities, specifically urban renewal and publicly assisted housing. In determining whether a county has the... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development...

  5. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... activities, specifically urban renewal and publicly assisted housing. In determining whether a county has the... 24 Housing and Urban Development 3 2011-04-01 2010-04-01 true Urban counties. 570.307 Section 570.307 Housing and Urban Development Regulations Relating to Housing and Urban Development...

  6. Public Health County of Santa Cruz

    E-print Network

    California at Santa Cruz, University of

    Public Health Division County of Santa Cruz HEALTH SERVICES AGENCY POST OFFICE BOX 962, 1080 at the two Public Health clinics located at 1080 Emeline Ave. in Santa Cruz and at 9 Crestview Dr. in Watsonville, on a walk-in basis. County health clinic services are limited to those who reside in Santa Cruz

  7. [A Profile of Kershaw County, South Carolina].

    ERIC Educational Resources Information Center

    Lilley, Stephen C.; McLean, Edward L.

    Careful long-range planning and slow, deliberate growth have benefitted Kershaw County's 500,000 pleasantly varied acres near the state capitol. The county, famous for its equestrian activity, boasts prestige stables, riding clubs, and trails. In addition there are lakes and numerous parks. Leaders want to maintain this aesthetic appeal while

  8. A Profile of Anson County, North Carolina.

    ERIC Educational Resources Information Center

    Farr, M. Gaston; And Others

    Since 1950 Anson County, North Carolina, has had major contributions to economic development, a source of great concern to residents of the almost entirely rural area. The increased capacity of the Blewitt Falls Dam power output and the county-wide water filtration system (one of only a few in the United States today) are attractive to industry.

  9. NORTH ST. LOUIS COUNTY SITES ANNUAL ENVIRONMENTAL

    E-print Network

    US Army Corps of Engineers

    REVISION 0 NORTH ST. LOUIS COUNTY SITES ANNUAL ENVIRONMENTAL MONITORING DATA AND ANALYSIS REPORT FOR CALENDAR YEAR 2014 ST. LOUIS, MISSOURI JUNE 30, 2015 U.S. Army Corps of Engineers St. Louis District Office Formerly Utilized Sites Remedial Action Program #12;#12;REVISION 0 NORTH ST. LOUIS COUNTY SITES ANNUAL

  10. NORTH ST. LOUIS COUNTY SITES ANNUAL ENVIRONMENTAL

    E-print Network

    US Army Corps of Engineers

    REVISION 0 NORTH ST. LOUIS COUNTY SITES ANNUAL ENVIRONMENTAL MONITORING DATA AND ANALYSIS REPORT FOR CALENDAR YEAR 2010 ST. LOUIS, MISSOURI JULY 8, 2011 U.S. Army Corps of Engineers St. Louis District Office Formerly Utilized Sites Remedial Action Program #12;#12;REVISION 0 NORTH ST. LOUIS COUNTY SITES ANNUAL

  11. NORTH ST. LOUIS COUNTY SITES ANNUAL ENVIRONMENTAL

    E-print Network

    US Army Corps of Engineers

    REVISION 0 NORTH ST. LOUIS COUNTY SITES ANNUAL ENVIRONMENTAL MONITORING DATA AND ANALYSIS REPORT FOR CALENDAR YEAR 2012 ST. LOUIS, MISSOURI JULY 19, 2013 U.S. Army Corps of Engineers St. Louis District Office Formerly Utilized Sites Remedial Action Program #12;#12;REVISION 0 NORTH ST. LOUIS COUNTY SITES ANNUAL

  12. NORTH ST. LOUIS COUNTY SITES ANNUAL ENVIRONMENTAL

    E-print Network

    US Army Corps of Engineers

    REVISION 0 NORTH ST. LOUIS COUNTY SITES ANNUAL ENVIRONMENTAL MONITORING DATA AND ANALYSIS REPORT FOR CALENDAR YEAR 2013 ST. LOUIS, MISSOURI JULY 23, 2014 U.S. Army Corps of Engineers St. Louis District Office Formerly Utilized Sites Remedial Action Program #12;#12;REVISION 0 NORTH ST. LOUIS COUNTY SITES ANNUAL

  13. Santa Clara County Asian Health Survey: Methodology.

    ERIC Educational Resources Information Center

    Tom, Soleng; And Others

    This paper describes the methodology used in a study assessing the health needs of the Asian population, specifically the Chinese, Cambodian, and Vietnamese populations, in Santa Clara County, California. The study by the Santa Clara County Health Department is part of a wide-ranging needs assessment project primarily intended to guide

  14. A Profile of Hardee County, Florida.

    ERIC Educational Resources Information Center

    Beaulieu, Lionel J.; Anderson, Deborah S.

    In 1977 leaders of Hardee County, Florida, listed relationships and attitudes of residents, rural atmosphere, environmental conditions, and economic potential among the county's strong points, and public service and facility improvements, developing economic potential, recreational and entertainment development, and planning and zoning as its most

  15. COUNTIES OF CALIFORNIA - WITH FARM STATISTICS

    EPA Science Inventory

    County farming statistics polygon coverage of California. The statistics were taken from the 1997 Census of Agriculture and added to a coverage extracted from TIGER county boundaries. A farm is any place from which $1,000 or more of agricultural products were sold, or normally w...

  16. Telephone Survey of Dade County Voters.

    ERIC Educational Resources Information Center

    Romanik, Dale G.

    The Dade County (Florida) Public Schools contracted with Strategy Research, Incorporated, to conduct a telephone survey of 400 Dade County registered voters. Focus was on determining the extent of support for a bond issue/tax increase to provide funds for the building and renovation of schools. Survey findings indicated that respondents: (1)

  17. A Profile of Suwannee County, Florida.

    ERIC Educational Resources Information Center

    Beaulieu, Lionel J.

    Agriculture and the railroad were significant forces in the development of Suwannee County, Florida, formally created in 1858 but explored and settled beginning some 300 years earlier. Lumber and cotton caused an early 20th century boom in the county which soon saw the negative effects of both industries. The introduction of tobacco in the late

  18. The Farm Crisis and Decatur County.

    ERIC Educational Resources Information Center

    Flora, Jan L.; And Others

    This case study assesses the impact of the farm sector on the economy and social organization of Decatur County (Kansas), a county which has historically depended on agriculture for its livelihood. Data were obtained from analysis of time series statistical indicators for the period between 1966 and 1984, questionnaire responses of local

  19. Latinos and Education in Ventura County.

    ERIC Educational Resources Information Center

    Segura, Denise A.

    This paper provides an overview of demographic and educational attainment data on Latinos in Ventura County, California, and describes an ongoing initiative to increase Latino college attendance through improved K-12 preparation and family involvement. In both its urban and rural areas, Ventura County harbors large Latino populations who are

  20. Analysis of County School Districts in Arkansas.

    ERIC Educational Resources Information Center

    Budd, Karol B.; Charlton, J.L.

    The 1948, Arkansas School District Reorganization Act was passed in an effort to reduce the 1589 small school districts to a smaller number. Those districts not consolidated would form county districts. As of the 1967-68 school year, 26 of these county districts remained. The purpose of this study was to provide information drawing attention to

  1. Geothermal development issues: Recommendations to Deschutes County

    SciTech Connect

    Gebhard, C.

    1982-07-01

    This report discusses processes and issues related to geothermal development. It is intended to inform planners and interested individuals in Deschutes County about geothermal energy, and advise County officials as to steps that can be taken in anticipation of resource development. (ACR)

  2. Groundwater management and protection Madison County, Alabama

    SciTech Connect

    French, J.H.; Strunk, J.W.

    1990-07-01

    Groundwater is extremely important to Madison County as it provides nearly three quarters of the county's drinking water. In recent years, Madison County has increasingly recognized the need to protect its groundwater resource. A supply of usable groundwater is one element of a high quality environment, which can help spur economic development and provide for the needs of a growing population. Without planning protection and understanding of possible consequences, however, economic development and population pressures can cause a gradual degradation of groundwater. In April 1987, the Tennessee Valley Authority (TVA) convened a local groundwater steering group in Madison County. At the first meeting the ground agreed upon these goals: (1) to seek incorporate groundwater protection into the planning and development process for Madison County, (2) to support efforts by Madison County to obtain authority to adopt zoning ordinances and subdivision regulations, and (3) to develop a groundwater management plan for the county. This report provides essential information needed in developing a plan and is based on the following assumptions: the citizens of Madison County value the environment in which they live and wish to protect it from pollution; continued economic development is necessary for a healthy local economy; and a healthy economy can be sustained and nurtured, without degradation of the groundwater resource, through countywide planning, education, and participation.

  3. Educational and Demographic Profile: Santa Cruz County

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Santa Cruz County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation provides a framework for enhanced communication and

  4. Educational and Demographic Profile: Yolo County

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Yolo County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced communication

  5. Commodity Flow Study - Clark County, Nevada, USA

    SciTech Connect

    Conway, S.Ph.D.; Navis, I.

    2008-07-01

    The United States Department of Energy has designated Clark County, Nevada as an 'Affected Unit of Local Government' due to the potential for impacts by activities associated with the Yucca Mountain High Level Nuclear Waste Repository project. Urban Transit, LLC has led a project team of transportation including experts from the University of Nevada Las Vegas Transportation Research Center to conduct a hazardous materials community flow study along Clark County's rail and truck corridors. In addition, a critical infrastructure analysis has also been carried out in order to assess the potential impacts of transportation within Clark County of high level nuclear waste and spent nuclear fuel to a proposed repository 90 miles away in an adjacent county on the critical infrastructure in Clark County. These studies were designed to obtain information relating to the transportation, identification and routing of hazardous materials through Clark County. Coordinating with the United States Department of Energy, the U.S. Department of Agriculture, the U. S. Federal Highway Administration, the Nevada Department of Transportation, and various other stakeholders, these studies and future research will examine the risk factors along the entire transportation corridor within Clark County and provide a context for understanding the additional vulnerability associated with shipping spent fuel through Clark County. (authors)

  6. First report of a clinical, multidrug-resistant Enterobacteriaceae isolate coharboring fosfomycin resistance gene fosA3 and carbapenemase gene blaKPC-2 on the same transposon, Tn1721.

    PubMed

    Li, Gang; Zhang, Ying; Bi, Dexi; Shen, Pinghua; Ai, Fuqi; Liu, Hong; Tian, Yueru; Ma, Yiming; Wang, Bei; Rajakumar, Kumar; Ou, Hong-Yu; Jiang, Xiaofei

    2015-01-01

    In order to understand the genetic background and dissemination mechanism of carbapenem resistance and fosfomycin resistance in Enterobacteriaceae isolates, we studied a clinical Escherichia coli strain HS102707 isolate and an Enterobacter aerogenes strain HS112625 isolate, both of which were resistant to carbapenem and fosfomycin and positive for the bla(KPC-2) and fosA3 genes. In addition, a clinical Klebsiella pneumoniae strain HS092839 isolate which was resistant to carbapenem was also studied. A 70-kb plasmid was successfully transferred to recipient E. coli J53 by a conjugation test. PCR and Southern blot analysis showed that bla(KPC-2) was located on this plasmid. The complete sequence of pHS102707 showed that this plasmid belongs to the P11 subfamily (IncP1) and has a replication gene, several plasmid-stable genes, an intact type IV secretion system gene cluster, and a composite transposon Tn1721-Tn3 that harbored bla(KPC-2). Interestingly, a composite IS26 transposon carrying fosA3 was inserted in the Tn1721-tnpA gene in pHS102707 and pHS112625, leading to the disruption of Tn1721-tnpA and the deletion of Tn1721-tnpR. However, only IS26 with a truncated Tn21-tnpR was inserted in pHS092839 at the same position. To our knowledge, this is the first report of fosA3 and bla(KPC-2) colocated in the same Tn1721-Tn3-like composite transposon on a novel IncP group plasmid. PMID:25367902

  7. Imperial County geothermal development annual meeting: summary

    SciTech Connect

    Not Available

    1983-01-01

    All phases of current geothermal development in Imperial County are discussed and future plans for development are reviewed. Topics covered include: Heber status update, Heber binary project, direct geothermal use for high-fructose corn sweetener production, update on county planning activities, Brawley and Salton Sea facility status, status of Imperial County projects, status of South Brawley Prospect 1983, Niland geothermal energy program, recent and pending changes in federal procedures/organizations, plant indicators of geothermal fluid on East Mesa, state lands activities in Imperial County, environmental interests in Imperial County, offshore exploration, strategic metals in geothermal fluids rebuilding of East Mesa Power Plant, direct use geothermal potential for Calipatria industrial Park, the Audubon Society case, status report of the Cerro Prieto geothermal field, East Brawley Prospect, and precision gravity survey at Heber and Cerro Prieto geothermal fields. (MHR)

  8. Environmental assessment: Deaf Smith County site, Texas

    SciTech Connect

    Not Available

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and the eight other potentially sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines. On the basis of these findings, the DOE is nominating the Deaf Smith County site as one of the five sites suitable for characterization. 591 refs., 147 figs., 173 tabs.

  9. lLLiad TN:

    E-print Network

    502509 lLL Number: 4009998 .... expressed in complex numbers, and is studied in detail in the field of high polymers. .... better; the vibrating plate of a telephone re- ...... than H, showing that Peter snow has a trace of horizontal layer structure.

  10. Association Nashville, TN

    E-print Network

    Berleant, Daniel

    interesting #12;History of The Future ''Study the past if you would divine the future.'' Confucius (from-Optimist Views Transhumanism (h+) Pic of kurzweil?pic of ,,when humans transcend...? Pic of movie? Exponential

  11. 75 FR 21983 - Standard Instrument Approach Procedures, and Takeoff Minimums and Obstacle Departure Procedures...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-27

    ...Fayetteville, TN, Fayetteville Muni, RNAV (GPS) RWY 20, Amdt 1A Paris, TN, Henry County, ILS OR LOC/NDB RWY 2, Amdt 1 Paris, TN, Henry County, NDB RWY 2, Amdt 3 Paris, TN, Henry County, RNAV (GPS) RWY 2, Orig Paris, TN,...

  12. 78 FR 68060 - Availability of FY 12 Grantee Performance Evaluation Reports for the Eight States of EPA Region 4...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ...County Land Use and Environmental Services Agency, NC; Western North Carolina Regional Air Quality Agency, NC; Chattanooga-Hamilton County Air Pollution Control Bureau, TN; Shelby County Health Department, TN; Knox County Department of Air Quality...

  13. 77 FR 52022 - Availability of FY 11 Grantee Performance Evaluation Reports for the Eight States of EPA Region 4...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-28

    ...County Land Use and Environmental Services Agency, NC; Western North Carolina Regional Air Quality Agency, NC; Chattanooga-Hamilton County Air Pollution Control Bureau, TN; Shelby County Health Department, TN; Knox County Department of Air Quality...

  14. Tn5-induced mutants of Azotobacter vinelandii affected in nitrogen fixation under Mo-deficient and Mo-sufficient conditions

    SciTech Connect

    Joerger, R.D.; Premakumar, R.; Bishop, P.E.

    1986-11-01

    Mutants of Azotobacter vinelandii affected in N/sub 2/ fixation in the presence of 1 ..mu..M Na/sub 2/MoO/sub 4/ (conventional system), 50 nM V/sub 2/O/sub 5/, or under Mo deficiency (alternative system) have been isolated after Tn5 mutagenesis with the suicide plasmid pSUP1011. These mutants are grouped into four broad phenotypic classes. Mutants in the first class are Nif/sup -/ under Mo sufficiency but Nif/sup +/ under Mo deficiency or in the presence of V/sub 2/O/sub 5/. Mutants in the second class are Nif/sup -/ under all conditions. An FeMo-cofactor-negative mutant (NifB/sup -/) belongs to this class. The third mutant class consists of mutants incapable of N/sub 2/-dependent growth under Mo deficiency. Most of the mutants of this class are also affected in N/sub 2/ fixation in the presence of 1 ..mu..M Na/sub 2/MoO/sub 4/, with acetylene reduction rates ranging from 28 to 51% of the rates of the wild type. Strains constructed by genetic transfer of the Kan/sup r/ marker of mutants from this class into nifHDK or nifK deletion mutants showed N/sub 2/-dependent growth only in the presence of V/sup 2/O/sub 5/. The only mutant in the fourth class shows wild-type nitrogenase activity under Mo sufficiency, but only 10% of the acetylene reduction activity of the wild type in the presence of 50 nM V/sub 2/O/sub 5/. The acetylene reduction rates of whole cells of this mutant are identical in Mo-deficient medium and in medium containing V/sub 2/O/sub 5/. The conventional nitrogenase subunits are expressed in this mutant even under Mo deficiency or in the presence of V/sub 2/O/sub 5/; however, the NH/sub 4//sup +/-and Mo-repressible proteins normally seen under these conditions could not be detected on two-dimensional gels.

  15. UF/IFAS Extension Hardee County February 2015

    E-print Network

    Hill, Jeffrey E.

    County Fair! Carolyn Hendry Wyatt UF/IFAS Interim County Extension Director Family & Consumer Science/4-HUF/IFAS Extension Hardee County NEWS February 2015 It's Fair Time! The month of February marks fair time for the Florida State Fair and the Hardee County Fair, and the two actually overlap by a few days

  16. 7 CFR 7.23 - County committee duties.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false County committee duties. 7.23 Section 7.23 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF FARM SERVICE AGENCY STATE AND COUNTY COMMITTEES 7.23 County committee duties. (a) The county committee, subject to the general direction and supervision of the State committee,...

  17. 7 CFR 7.25 - County executive director duties.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false County executive director duties. 7.25 Section 7.25 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF FARM SERVICE AGENCY STATE AND COUNTY COMMITTEES 7.25 County executive director duties. (a) The county executive director will execute the policies established by the...

  18. Costilla County Biodiesel Pilot Project

    SciTech Connect

    Doon, Ben; Quintana, Dan

    2011-08-25

    The Costilla County Biodiesel Pilot Project has demonstrated the compatibility of biodiesel technology and economics on a local scale. The project has been committed to making homegrown biodiesel a viable form of community economic development. The project has benefited by reducing risks by building the facility gradually and avoiding large initial outlays of money for facilities and technologies. A primary advantage of this type of community-scale biodiesel production is that it allows for a relatively independent, local solution to fuel production. Successfully using locally sourced feedstocks and putting the fuel into local use emphasizes the feasibility of different business models under the biodiesel tent and that there is more than just a one size fits all template for successful biodiesel production.

  19. Characteristics of the Las Vegas/Clark County visitor economy

    SciTech Connect

    1988-06-01

    The purpose of this report is to present the results of a review of the Clark County visitor economy and the Clark County visitor. The review, undertaken in support of NWPO`s two objectives mentioned above, addressed a number of topics including performance of the Clark County visitor economy as a generator of employment, earnings and tax base; importance of the Clark County visitor economy to the Nevada economy as a whole; elements of the Clark County visitor economy outside the Las Vegas strip and downtown areas; current trends in the Clark County visitor industry; and indirect economic effects of Clark County casino/hotel purchases.

  20. Presidio County State of the Community Reprort

    E-print Network

    Xiao, Dong; Hwang, Jinuk; McCharen, Rob

    2015-01-01

    stream_source_info Jinuk Robert Dong_Presidio SOC Poster Final.pdf.txt stream_content_type text/plain stream_size 6288 Content-Encoding UTF-8 stream_name Jinuk Robert Dong_Presidio SOC Poster Final.pdf.txt Content-Type text... dividing it into 3 other counties giving way to its current shape, covering 3,856 square miles making it the fourth largest county in Texas. The population fluctuated with the coming and going of US Troops supported at two Army bases in the county from...

  1. Tri-county pilot study. [Texas

    NASA Technical Reports Server (NTRS)

    Reeves, C. A. (principal investigator); Austin, T. W.; Kerber, A. G.

    1976-01-01

    The author has identified the following significant results. An area inventory was performed for three southeast Texas counties (Montgomery, Walker, and San Jacinto) totaling 0.65 million hectares. The inventory was performed using a two level hierarchy. Level 1 was divided into forestland, rangeland, and other land. Forestland was separated into Level 2 categories: pine, hardwood, and mixed; rangeland was not separated further. Results consisted of area statistics for each county and for the entire study site for pine, hardwood, mixed, rangeland, and other land. Color coded county classification maps were produced for the May data set, and procedures were developed and tested.

  2. 1918 influenza: a Winnebago County, Wisconsin perspective.

    PubMed

    Shors, Teri; McFadden, Susan H

    2009-12-01

    The population of Winnebago County in 1918 was approximately 62,000 residents. It consisted of towns supporting diverse manufacturers surrounded by farming country. For this study, records were revisited, and 1918 to 1920 influenza survivors were interviewed. A pharmacological investigation encompassing the various influenza treatments used in Wisconsin from 1918 to 1920 was documented. In 1918, over 180 individuals perished from influenza, and over 2000 cases were reported in Winnebago County, Wisconsin. Influenza returned in 1920, which some researchers refer to as the "fourth wave," claiming nearly 50 lives in Winnebago County, Wisconsin. This study also documents the 1920 influenza wave. PMID:19889943

  3. 1918 Influenza: A Winnebago County, Wisconsin Perspective

    PubMed Central

    Shors, Teri; McFadden, Susan H.

    2009-01-01

    The population of Winnebago County in 1918 was approximately 62,000 residents. It consisted of towns supporting diverse manufacturers surrounded by farming country. For this study, records were revisited, and 1918 to 1920 influenza survivors were interviewed. A pharmacological investigation encompassing the various influenza treatments used in Wisconsin from 1918 to 1920 was documented. In 1918, over 180 individuals perished from influenza, and over 2000 cases were reported in Winnebago County, Wisconsin. Influenza returned in 1920, which some researchers refer to as the fourth wave, claiming nearly 50 lives in Winnebago County, Wisconsin. This study also documents the 1920 influenza wave. PMID:19889943

  4. Drainage basins in Duval County, Florida

    USGS Publications Warehouse

    Stone, Roy B.; Largen, Joseph B.

    1983-01-01

    The drainage basins and subbasins in Duval County, Florida, are delineated on this atlas map. The county 's 840 square-mile area is drained by three major river systems; the St. Johns, 668 square miles; Nassau, 113 square miles; and St. Marys, 59 square miles. The remainder of the county is drained by a network of small streams that flow into either the Intracoastal Waterway or directly into the Atlantic Ocean. The sub-basins range in size from less than one square mile to more than 50 square miles. (USGS)

  5. Lea County 001, an H5 chondrite, and Lea County 002, an ungrouped type 3 chondrite

    SciTech Connect

    Zolensky, M.E.; Score, R.; Clayton, R.N.; Mayeda, T.K.; Schutt, J.W. Lockheed Engineering and Sciences Co., Houston, TX Chicago Univ., IL )

    1989-12-01

    A search of active deflation basins near Jal, Lea County, New Mexico resulted in the discovery of two meteorites, Lea County 001 and 002. Lea County 001 has mean olivine and low-Ca pyroxene compositions of Fa(19) and Fs(17), respectively. These and all other mineralogical and petrological data collected indicate a classification of H5 for this stone. Lea County 002 has mean olivine and low-Ca pyroxene compositions of Fa(2) and Fs(4), and is unequilibrated. Although it is mineralogically most similar to Kakangari and chondritic clasts within Cumberland Falls, the high modal amount of forsterite makes Lea County a unique type 3 chondrite. Oxygen isotope data for Lea County 002 fall on an 0-16-mixing line through those of the enstatite meteorites and IAB irons, a feature shared by Kakangari. 28 refs.

  6. A novel arginine to tryptophan (R144W) mutation in troponin T (cTnT) gene in an indian multigenerational family with dilated cardiomyopathy (FDCM).

    PubMed

    Rani, Deepa Selvi; Dhandapany, Perundurai S; Nallari, Pratibha; Narasimhan, Calambur; Thangaraj, Kumarasamy

    2014-01-01

    Cardiomyopathy is a major cause of heart failure and sudden cardiac death; several mutations in sarcomeric protein genes have been associated with this disease. Our aim in the present study is to investigate the genetic variations in Troponin T (cTnT) gene and its association with dilated cardiomyopathy (DCM) in south-Indian patients. Analyses of all the exons and exon-intron boundaries of cTnT in 147 DCM and in 207 healthy controls had revealed a total of 15 SNPs and a 5 bp INDEL; of which, polymorphic SNPs were compared with the HapMap population data. Interestingly, a novel R144W mutation, that substitutes polar-neutral tryptophan for a highly conserved basic arginine in cTnT, altering the charge drastically, was identified in a DCM, with a family history of sudden-cardiac death (SCD). This mutation was found within the tropomyosin (TPM1) binding domain, and was evolutionarily conserved across species, therefore it is expected to have a significant impact on the structure and function of the protein. Family studies had revealed that the R144W is co-segregating with disease in the family as an autosomal dominant trait, but it was completely absent in 207 healthy controls and in 162 previously studied HCM patients. Further screening of the proband and three of his family members (positive for R144W mutant) with eight other genes ?-MYH7, MYBPC3, TPM1, TNNI3, TTN, ACTC, MYL2 and MYL3, did not reveal any disease causing mutation, proposing the absence of compound heterozygosity. Therefore, we strongly suggest that the novel R144W unique/private mutant identified in this study is associated with FDCM. This is furthermore signifying the unique genetic architecture of Indian population. PMID:24992688

  7. An Oral Fayette County, Texas Rural School History: Perspectives of a County Superintendent and a Long-Time Teacher.

    ERIC Educational Resources Information Center

    Linden, Mary Frances

    This paper represents a brief history of rural education in Fayette County (Texas) from 1918 through 1975. The paper focuses on the personal narratives of Harry Loeb, the last official superintendent of Fayette County and Emma Diedt, a 50-year Fayette County teacher. The narratives describe Fayette County schools, school politics, and the

  8. 75 FR 49016 - County of Greenville, S.C.-Acquisition Exemption-Greenville County Economic Development Corporation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ... Greenville County Economic Development Corporation (GCEDC) approximately 11.8 miles of rail line between... accordance with the National Trails System Act, 16 U.S.C. 1247(d). See Greenville County Economic Development... Surface Transportation Board County of Greenville, S.C.--Acquisition Exemption--Greenville County...

  9. 78 FR 60686 - Establishment of the Big Valley District-Lake County and Kelsey Bench-Lake County Viticultural...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    ... on April 5, 2013 (78 FR 20544), proposing to establish the Big Valley District-Lake County and Kelsey... District-Lake County and Kelsey Bench-Lake County Viticultural Areas and Modification of the Red Hills Lake... approximately 11,000-acre ``Big Valley District-Lake County'' viticultural area and the approximately...

  10. 76 FR 30152 - East Calloway County Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... AGENCY East Calloway County Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of... response costs concerning the East Calloway County Middle School Mercury Spill Site located in Murray... Site name East Calloway County ] Middle School Mercury Spill Site by one of the following methods:...

  11. The Soils of Bowie, Denton, Freestone, and Red River Counties.

    E-print Network

    Fraps, G. S. (George Stronach)

    1928-01-01

    in Denton County. The subsoils are likewise low in plant food and they are more acid than the surface soils. The upland prairie soils are better supplied with plant food and with lime than the forested soils. They contain two or three times as much... of analyses of soils averaged by groups. - upland (Forested) Surface Soil Bowie County. .................................. Denton County (Cross Timbers). .................. Freestone County. ............................... Red River County...

  12. 69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C.L. Wood, the county engineer. Updated through the mid-1930s to show new federal aid-state roads. Compares modern system with older county system. Original scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

  13. A novel gene, ardD, determines antirestriction activity of the non-conjugative transposon Tn5053 and is located antisense within the tniA gene

    PubMed Central

    Balabanov, Vladimir P; Kotova, Vera Yu; Kholodii, Gennady Y; Mindlin, Sofia Z; Zavilgelsky, Gennadii B

    2012-01-01

    Abstract The mercury-resistance transposon Tn5053 inhibits restriction activity of the type I restriction-modification endonuclease EcoKI in Escherichia coli K12 cells. This is the first report of antirestriction activity of a non-conjugative transposon. The gene (ardD) coding for the antirestriction protein has been cloned. The ardD gene is located within the tniA gene, coding for transposase, on the complementary strand. The direction of transcription is opposite to transcription of the tniA gene. PMID:22967207

  14. Prognostic Role of Adjuvant Chemotherapy in Node-Negative (N0), Triple-Negative (TN), Medullary Breast Cancer (MBC) in the Korean Population

    PubMed Central

    Lim, SeungTaek; Park, Se Ho; Park, Heong Kyu; Hur, Min Hee; Oh, Se Jeong; Suh, Young Jin

    2015-01-01

    Background Despite the favorable prognosis for medullary breast cancer (MBC), the guidelines for the use of adjuvant chemotherapy for MBC have not been clearly established. This study investigated the prognostic role of adjuvant chemotherapy in Korean patients with node-negative (N0), triple-negative (TN) MBC patients. Methods We included data from 252 patients with N0 TN MBC, obtained from the Korean Breast Cancer Registry database. Patients were categorized as those who did not undergo adjuvant chemotherapy (group I) or those who did (group II). Clinicopathological characteristics, breast cancer-specific survival (BCSS), and overall survival (OS) were compared between the groups. In addition, a subgroup analysis for survival based on tumor size was conducted. Results A total of 252 N0 TN MBC patients with tumor sizes >1 cm who were diagnosed between April 1997 and March 2011 were enrolled. The median age was 44.95 years (range, 2572 years), and the median follow-up period was 93.94 months (range, 23195 months). Overall, the BCSS and OS in group II (97.3% and 97.3%, respectively) were significantly better compared with those in group I (89.2% and 86.2%, respectively). In the subgroup analysis, in patients with tumors >2 cm in size, those in group II had significant better BCSS and OS (97.5% and 97.5%, respectively) compared with those in group I (78.3% and 73.9%, respectively). In contrast in those with tumors 12 cm in size, there were no significant differences in BCSS and OS between the groups (both 97.1% for group I, and 95.2% and 92.9%, respectively for group II). Multivariate analysis revealed that adjuvant chemotherapy significantly improved BCSS (P = 0.009) and OS (P = 0.007), but only for patients with larger tumors (>2 cm). Conclusions In patients with N0 TN MBC, adjuvant chemotherapy had a significant clinical survival benefit, but only in those with tumors >2 cm. PMID:26562837

  15. Charging Up in King County, Washington

    SciTech Connect

    Constantine, Dow; Oliver, LeAnn; Inslee, Jay; Sahandy, Sheida; Posthuma, Ron; Morrison, David;

    2011-01-01

    King County, Washington is spearheading a regional effort to develop a network of electric vehicle charging stations. It is also improving its vehicle fleet and made significant improvements to a low-income senior housing development.

  16. Environmental assessment, Deaf Smith County site, Texas

    SciTech Connect

    Not Available

    1986-05-01

    The Nuclear Waste Policy Act of 1982 (42 USC sections 10101-10226) requires the environmental assessment of a proposed site to include a statement of the basis for nominating a site as suitable for characterization. Volume 2 provides a detailed statement evaluating the site suitability of the Deaf Smith County Site under DOE siting guidelines, as well as a comparison of the Deaf Smith County Site to the other sites under consideration. The evaluation of the Deaf Smith County Site is based on the impacts associated with the reference repository design, but the evaluation will not change if based on the Mission Plan repository concept. The second part of this document compares the Deaf Smith County Site to Davis Canyon, Hanford, Richton Dome and Yucca Mountain. This comparison is required under DOE guidelines and is not intended to directly support subsequent recommendation of three sites for characterization as candidate sites. 259 refs., 29 figs., 66 refs. (MHB)

  17. DUST TRANSPORT IN MARICOPA COUNTY, ARIZONA

    EPA Science Inventory

    Numerical simulations have been carried out for ambient air supermicrometric particulate concentrations in Maricopa County, Arizona during late fall and winter periods of atmospheric stability. Results of model studies are in approximate agreement with limited field observational...

  18. 29 CFR 780.812 - County.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...UNDER THE FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) County...

  19. 29 CFR 780.812 - County.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...UNDER THE FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) County...

  20. 29 CFR 780.812 - County.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...UNDER THE FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) County...

  1. Charging Up in King County, Washington

    ScienceCinema

    Constantine, Dow; Oliver, LeAnn; Inslee, Jay; Sahandy, Sheida; Posthuma, Ron; Morrison, David;

    2013-05-29

    King County, Washington is spearheading a regional effort to develop a network of electric vehicle charging stations. It is also improving its vehicle fleet and made significant improvements to a low-income senior housing development.

  2. Jefferson County State of the Community Report

    E-print Network

    Chen, Xi; Lee, Kanghyun; Aldalbahi, Faisal

    2015-01-01

    of Incorporation 1861 Square Miles 773 Square Miles Unincorporated Je?erson County Area 653 Miles Percentage days of Sunshine 70% sunshine Hottest Month July (74 degree average) Coldest Month January (30 degrees average) Annual Precipitation 15.4 inches...

  3. Environmental assessment: Deaf Smith County site, Texas

    SciTech Connect

    Not Available

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of the nine potentially acceptable sites for mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines.

  4. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...assistance activities means community renewal and lower income housing activities, specifically urban renewal and publicly assisted housing. In determining... (1) The qualification by HUD of an urban county shall remain effective for...

  5. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...assistance activities means community renewal and lower income housing activities, specifically urban renewal and publicly assisted housing. In determining... (1) The qualification by HUD of an urban county shall remain effective for...

  6. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...assistance activities means community renewal and lower income housing activities, specifically urban renewal and publicly assisted housing. In determining... (1) The qualification by HUD of an urban county shall remain effective for...

  7. Digital geologic map of Texas County, Oklahoma

    USGS Publications Warehouse

    Cederstrand, J.R.

    1997-01-01

    This data set consists of digital data and accompanying documentation for the surficial geology of Texas County, Oklahoma. The original data are from the Hydrogeologic Map, sheet 1 of 3, included in the U.S. Geological Survey publication, Availability of ground water in Texas County, Oklahoma, Hydrologic Investigations Atlas HA-250, Wood and Hart, 1967. The geology was compiled by S.L. Schoff, 1939 and 1953.

  8. The Water Cycle in Volusia County

    USGS Publications Warehouse

    German, Edward R.

    2009-01-01

    Earth's water is always in motion. The water cycle, also known as the hydrologic cycle, describes the continuous movement of water on, above, and below the Earth's surface. This fact sheet provides information about how much water moves into and out of Volusia County, and where it is stored. It also illustrates the seasonal variation in water quantity and movement using data from some of the hydrologic data collection sites in or near Volusia County, Florida.

  9. SOLAR PANELS ON HUDSON COUNTY FACILITIES

    SciTech Connect

    BARRY, KEVIN

    2014-06-06

    This project involved the installation of an 83 kW grid-connected photovoltaic system tied into the energy management system of Hudson County's new 60,000 square foot Emergency Operations and Command Center and staff offices. Other renewable energy features of the building include a 15 kW wind turbine, geothermal heating and cooling, natural daylighting, natural ventilation, gray water plumbing system and a green roof. The County intends to seek Silver LEED certification for the facility.

  10. Water resources of Langlade County, Wisconsin

    USGS Publications Warehouse

    Batten, W. G.

    1987-01-01

    An average of about 4.7 million gallons of water was pumped daily in Langlade County in 1983. Irrigation and fish rearing are the major ground-water uses in the county. An average of about 4.2 million gallons per day was pumped for irrigation during the months of June, July, and August. Results of this study show that present irrigation pumpage rates have little effect on groundwater levels in the Antigo Flats area.

  11. 76 FR 30025 - Revisions to the California State Implementation Plan, Placer County Air Pollution Control...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ...Implementation Plan, Placer County Air Pollution Control District and Ventura County Air Pollution Control District AGENCY: Environmental...revisions to the Placer County Air Pollution Control District (PCAPCD)...

  12. 76 FR 30080 - Revisions to the California State Implementation Plan, Placer County Air Pollution Control...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ...Implementation Plan, Placer County Air Pollution Control District and Ventura County Air Pollution Control District AGENCY: Environmental...revisions to the Placer County Air Pollution Control District (PCAPCD)...

  13. The flora of Oktibbeha County, Mississippi

    USGS Publications Warehouse

    Leidolf, A.; McDaniel, S.; Nuttle, T.

    2002-01-01

    We surveyed the flora of Oktibbeha County, Mississippi, U.S.A., from February 1994 to 1996. Occupying 118 square kilometers in east-central Mississippi, Oktibbeha County lies among 3 physiographic regions that include, from west to east, Interior Flatwoods, Pontotoc Ridge, and Black Prairie. Accordingly, the county harbors a diverse flora. Based on field work, as well as an extensive review of published literature and herbarium records at IBE and MISSA, we recorded a total of 1,148 taxa (1,125 species, 7 hybrids, 16 infraspecific taxa) belonging to 514 genera in 160 families, over 85% of all taxa documented were native. Compared to 3 other counties in east-central Mississippi, Oktibbeha County has the second largest recorded flora. The number of state-listed (endangered, threatened, or of special concern) taxa (67) documented in this survey far exceeds that reported from any other county in the region. Three introduced species, Ilex cornuta Lindl. & Paxton, Mahonia bealei (Fortune) Carrie??re, and Nandina domestica Thunb., are reported in a naturalized state for the first time from Mississippi. We also describe 16 different plant communities belonging to 5 broad habitat categories: bottomland forests, upland forests and prairies, aquatic habitats, seepage areas, and human-influenced habitats. A detailed description of the vegetation associated with each of these communities is provided.

  14. 76 FR 38809 - Federal Property Suitable as Facilities To Assist the Homeless

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ...Bldgs. Fort McClellan Ft. McClellan Co: Calhoun AL 36205-5000 Landholding Agency: Army...deterioration 11 Bldgs., Anniston Army Depot Calhoun AL 36201 Landholding Agency: Army Property...Secured Area Bldgs. 00001, 00003, 00030 John Sevier Range Knoxville TN 37918...

  15. 75 FR 51535 - Federal Property Suitable as Facilities to Assist the Homeless

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-20

    ...Bldgs. Fort McClellan Ft. McClellan Co: Calhoun AL 36205-5000 Landholding Agency: Army...deterioration 11 Bldgs. Anniston Army Depot Calhoun AL 36201 Landholding Agency: Army Property...deterioration Bldgs. 00001, 00003, 00030 John Sevier Range Knoxville TN 37918...

  16. 75 FR 13347 - Federal Property Suitable as Facilities To Assist the Homeless

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ...01271, Fort McClellan Ft. McClellan Co: Calhoun AL 36205-5000 Landholding Agency: Army...deterioration 10 Bldgs., Anniston Army Depot Calhoun AL 36201 Landholding Agency: Army Property...deterioration Bldgs. 00001, 00003, 00030 John Sevier Range Knoxville TN 37918...

  17. Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms

    PubMed Central

    van Opijnen, Tim; Lazinski, David W.; Camilli, Andrew

    2015-01-01

    The lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobials and vaccines. Here we present the method Tn-seq, with which it has become possible to quantitatively determine fitness for most genes in a microorganism and to screen for quantitative genetic interactions on a genome-wide scale and in a high-throughput fashion. Tn-seq can thus direct studies in the annotation of genes and untangle complex phenotypes. The method is based on the construction of a saturated transposon insertion library. After library selection, changes in frequency of each insertion mutant are determined by sequencing of the flanking regions en masse. These changes are used to calculate each mutant's fitness. The method was originally developed for the Gram-positive bacterium Streptococcus pneumoniae, a causative agent of pneumonia and meningitis, but has now been applied to several different microbial species. PMID:24733243

  18. A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I.

    PubMed Central

    Davies, C J; Hutchison, C A

    1991-01-01

    We have developed a directed DNA sequencing strategy based upon the Escherichia coli transposon Tn3. This transposon displays little sequence specificity for transposition and is thus well suited to this task. Both mini-Tn3 transposons and sequencing vectors bearing the phage f1 single stranded origin of replication have been constructed. Upon mutagenesis of a target sequence, a population is produced in which each clone has two f1 origins of replication, one of which is at a variable position depending upon the transposon insertion site. When helper phage is added to the mutagenised population, the two f1 origins present on each clone are nicked, dividing the packaged strand into two segments, each of which is packaged into a separate phage particle. One of these segments contains no resistance markers and is lost, whilst the other is recovered as a deleted clone with a single chimeric f1 origin. A unidirectionally, variably-deleted set of sequencing clones is produced, and appropriately sized clones are sequenced using a primer complimentary to the transposon end. In addition to being inexpensive, the method does not require the same degree of technical expertise needed for many in vitro, enzymatically based methods. The strategy has been used to determine 2.6 kilobases of nucleotide sequence in the Saccharomyces cerevisiae ADE 1 locus. Images PMID:1658741

  19. Estimation methods and monitoring network issues in the quantitative estimation of land-based COD and TN loads entering the sea: a case study in Qingdao City, China.

    PubMed

    Su, Ying; Wang, Xiulin; Li, Keqiang; Liang, Shengkang; Qian, Guodong; Jin, Hong; Dai, Aiquan

    2014-09-01

    At present, the monitoring network of China cannot provide sufficient data to estimate land-based pollutant loads that enter the sea, and estimation methods are imprecisely used. In this study, the selection of monitoring stations, monitoring frequency, and pollutant load estimation methods was studied in Qingdao City, a typical coastal city in China, taken as an example. Land-based pollutant loads from Qingdao were estimated, and load distribution, density, and composition were analyzed to identify the key pollution source regions (SRs) that need to be monitored and controlled. Results show that the administrative land area of Qingdao can be divided into 25 sea-sink source regions (SSRs). A total of 14 more rivers and 62 industrial enterprises should be monitored to determine the comprehensive pollutant loads of the city. Furthermore, the monitoring frequency of rivers should not be less than three times/year; a monitoring frequency of five or more times is preferable. The findings on pollutant load estimation with the use of different estimation methods substantially vary; estimation results with the use of ratio-based methods were 10 and 22 % higher than those with the use of monitoring-based methods in terms of chemical oxygen demand (COD) and total nitrogen (TN), respectively. None-point sources contributed the majority of the pollutant loads at about 70 % of the total COD and 60 % of the total TN. PMID:24865505

  20. Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms

    PubMed Central

    van Opijnen, Tim; Lazinski, David W.; Camilli, Andrew

    2015-01-01

    The lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobial agents and vaccines. This unit presents Tn-seq, a method that has made it possible to quantitatively determine fitness for most genes in a microorganism and to screen for quantitative genetic interactions on a genome-wide scale and in a high-throughput fashion. Tn-seq can thus direct studies on the annotation of genes and untangle complex phenotypes. The method is based on the construction of a saturated transposon insertion library. After library selection, changes in the frequency of each insertion mutant are determined by sequencing flanking regions en masse. These changes are used to calculate each mutants fitness. The method was originally developed for the Gram-positive bacterium Streptococcus pneumoniae, a causative agent of pneumonia and meningitis, but has now been applied to several different microbial species. PMID:25641100

  1. Citrus County Schools Copyright Guidelines Recommended by the Citrus County Association of School Media Specialists.

    ERIC Educational Resources Information Center

    Citrus County School District, Inverness, FL.

    This document contains copyright guidelines determined appropriate for the Citrus County School System (Florida) by the Citrus County Association of School Media Specialists in May, 1992. These guidelines are based on interpretation and understanding of current copyright law as applied to education and implemented in school districts in the United

  2. 77 FR 16318 - Environmental Assessment: Jessamine County and Madison County, KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ...Record of Decision (ROD) for a new connector between US 27 in Jessamine County, KY...SAFETEA-LU. The purpose of the 1-75 Connector project is to improve regional access...Record of Decision (ROD) for a new connector between US 27 in Jessamine County,...

  3. 75 FR 13297 - Southeastern Lincoln County Habitat Conservation Plan, Lincoln County, NV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... intent to prepare an EIS for this project in the Federal Register on July 5, 2001 (66 FR 35451), and held..., and draft IA in the Federal Register (73 FR 74185). The draft documents were available for a 75-day... Fish and Wildlife Service Southeastern Lincoln County Habitat Conservation Plan, Lincoln County,...

  4. 75 FR 31463 - Comal County Regional Habitat Conservation Plan, Comal County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... Federal Register on October 16, 2008 (73 FR 61433). A public scoping meeting was held on December 4, 2008... Fish and Wildlife Service Comal County Regional Habitat Conservation Plan, Comal County, TX AGENCY... statement, draft habitat conservation plan, and permit application; announcement of a public...

  5. Constraints of Implementing Free Secondary Education in Mandera West Sub-County, Mandera County, Kenya

    ERIC Educational Resources Information Center

    Adan, Mohammed Abdi; Orodho, John Aluko

    2015-01-01

    This study sought to find out the constraints of implementing free secondary education (FSE) in secondary schools in Mandera West Sub-County, Mandera County, Kenya. The study is based on the theory of constraints as the researcher examines the factors constraining the achievement of FSE objectives. The study used the survey design. The main

  6. Beginnings of geothermal impact on county population and leadership, Imperial County, California

    SciTech Connect

    Pick, J.B.; Butler, E.W.

    1980-09-01

    A major geothermal energy development scenario is about to begin in Imperial County. Initial energy-socioeconomic interactions in the areas of population and county leadership structure are discussed. These include immigration of energy company workers, a sewage plant dispute, conflict over union affiliation of geothermal laborers, and a transmission corridor routing dispute.

  7. STATE AND COUNTY PLANNING OF A PROGRAM OF SERVICES FOR A COUNTY SCHOOLS OFFICE.

    ERIC Educational Resources Information Center

    MARCHUS, FLOYD

    THE COUNTY SCHOOLS OFFICE IS A STATE AGENCY WITH CONSIDERABLE LOCAL AUTONOMY. IT IS IN A UNIQUE POSITION TO ADMINISTER SERVICES TO SCHOOL DISTRICTS AND TO COORDINATE COOPERATIVE ACTIVITIES AMONG SCHOOL DISTRICTS, THEREBY HELPING TO ENSURE EQUALIZATION OF EDUCATIONAL OPPORTUNITIES. A REVIEW OF CONTRA COSTA COUNTY'S SCHOOLS OFFICE REVEALS 12

  8. Geology of Griggs and Steele Counties

    USGS Publications Warehouse

    Bluemle, John P.

    1975-01-01

    Griggs and Steele Counties, located at the eastern edge of the Williston basin, are underlain by 400 to 2,600 feet of Paleozoic and Mesozoic rocks that dip gently to the west. The Cretaceous Greenhorn, Carlile, Niobrara, and Pierre Formations lie directly beneath the glacial drift, and shale of the Pierre Formation is exposed in several places along the Sheyenne River. The Pleistocene Coleharbor Formation, which covers most of the area, consists mainly of glacial, fluvial, and lake sediment. The Coleharbor Formation averages 200 to 300 feet thick, but it is as much as 550 feet thick in some of the buried valleys. The Holocene Walsh Formation occurs in parts of the area, chiefly sloughs and river bottomland. It consists mainly of alluvial and eolian sediment. Griggs County and the western two-thirds of Steele County are part of the Drift Prairie, which is characterized by flat to gently rolling topography that is rugged in areas of end moraines and intense ice thrusting, subdued on the ground moraine and outwash plains. Associated with these major landforms are numerous washboard moraines, drumlins, eskers, kames, meltwater trenches, and water-washed areas. The eastern third of Steele County is a nearly flat area covered by lake deposits of the glacial Lake Agassiz. As the Late Wisconsinan glacier in eastern North Dakota thinned and receded eastward, it was increasingly affected by the topography over which it was flowing. This resulted in lobation of the glacier. Locally intense areas of thrusting developed within the lobate glacier, and large blocks of subglacial material were moved short distances. Large areas of Griggs County were washed by water flowing from the glacier, and in some areas gravel and sand were deposited. Continued withdrawal of the glacier resulted in ponding of melt water in parts of the two counties. These and other ponds tended to coalesce at lower and lower elevations, eventually forming Lake Agassiz, which flooded part of eastern Steele County.

  9. 77 FR 12830 - Pershing County Water Conservation District; Notice of Intent To File License Application, Filing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-02

    ...14327-000] Pershing County Water Conservation District; Notice of Intent...Submitted by: Pershing County Water Conservation District (Pershing County...Bennie Hodges, Pershing County Water Conservation District, P.O. Box...

  10. Dutchess County Resource Recovery Task Force report: Dutchess County Pyrolysis Program

    SciTech Connect

    1980-07-01

    Dutchess County initiated development of a long-range master plan for Solid Waste Management in 1971. The plan included development of a resource recovery facility to service the municipalities in the County population center. Based on early recommendations, a pyrolysis facility employing Purox technology was to be implemented. A feasibility study, paid for by County funds was completed in 1975. The study provided siting recommendations, estimation of available waste, and preliminary facility design. Because of various considerations, the project was not developed. Under the Department of Energy grant, the County reassessed the feasibility of a resource recovery facility, with emphasis on confirming previous conclusions supporting the Purox technology, waste availability, energy recovery and sale and siting of the plant. The conclusions reached in the new study were: a resource recovery facility is feasible for the County; sufficient waste for such a facility is available and subject to control; While Purox technology was feasible it is not the most appropriate available technoloy for the County; that mass burning with steam recovery is the most appropriate technology; and that resource recovery while presently more expensive than landfilling, represents the only cost effective, energy efficient, and environmentally sound way to handle the solid waste problem in the County.

  11. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...The Red Hill Douglas County, Oregon viticultural area is located in Douglas County, Oregon, east of Interstate 5 near the hamlet of Rice Hill, between the villages of Yoncalla and Oakland. (1) Beginning on the Yoncalla map along the...

  12. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...The Red Hill Douglas County, Oregon viticultural area is located in Douglas County, Oregon, east of Interstate 5 near the hamlet of Rice Hill, between the villages of Yoncalla and Oakland. (1) Beginning on the Yoncalla map along the...

  13. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...The Red Hill Douglas County, Oregon viticultural area is located in Douglas County, Oregon, east of Interstate 5 near the hamlet of Rice Hill, between the villages of Yoncalla and Oakland. (1) Beginning on the Yoncalla map along the...

  14. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...The Red Hill Douglas County, Oregon viticultural area is located in Douglas County, Oregon, east of Interstate 5 near the hamlet of Rice Hill, between the villages of Yoncalla and Oakland. (1) Beginning on the Yoncalla map along the...

  15. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...The Red Hill Douglas County, Oregon viticultural area is located in Douglas County, Oregon, east of Interstate 5 near the hamlet of Rice Hill, between the villages of Yoncalla and Oakland. (1) Beginning on the Yoncalla map along the...

  16. 76 FR 7810 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-11

    ...DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee...SUMMARY: The Big Horn County Resource Advisory Committee...ADDRESSES: The meeting will be held at the Big Horn Federal Savings Bank, 8 East...

  17. Los Angeles County Poor Farm, Patient Ward Nos. 210 & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Los Angeles County Poor Farm, Patient Ward Nos. 210 & 211 - Type B Plan, 7601 Imperial Highway; bounded by Esperanza Street, Laurel Street, Flores Street, and Descanso Street, Downey, Los Angeles County, CA

  18. 28. Photocopy of drawing (original in possession of Allegany County, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Photocopy of drawing (original in possession of Allegany County, Cumberland, MD) FACTORY BUILDING, PLAN OF FLOOR B, MACHINERY LAYOUT, 1943, NOTE INFILLED COURTS - Kelly-Springfield Tire Plant, Factory Building, 701 Kelly Road, Cumberland, Allegany County, MD

  19. 27. Photocopy of drawing (original in possession of Allegany County, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. Photocopy of drawing (original in possession of Allegany County, Cumberland, MD) FACTORY BUILDING, PLAN OF FLOOR B, 1928, NOTE OPEN COURTS - Kelly-Springfield Tire Plant, Factory Building, 701 Kelly Road, Cumberland, Allegany County, MD

  20. 78 FR 68135 - Environmental Impact Statement: Los Angeles County, California

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ...Administration Environmental Impact Statement: Los Angeles County, California AGENCY: Federal...prepared for a proposed highway project in Los Angeles County, California. DATES: Public...Transportation (Caltrans), 100 S. Main Street, Los Angeles, CA 90012, telephone (213)...

  1. 76 FR 13017 - Environmental Impact Statement: Los Angeles County, CA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-09

    ...Administration Environmental Impact Statement: Los Angeles County, CA AGENCY: Federal Highway...prepared for a proposed highway project in Los Angeles County, California. DATES: Public...Gymnasium), 2241 N. Eastern Ave., Los Angeles, CA 90032. Pasadena--Lake...

  2. Los Angeles County Poor Farm, Patient Wards 201, 202, 203, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Los Angeles County Poor Farm, Patient Wards 201, 202, 203, 204, 205, 206, 207, 208 & 209 - Type A Plan, 7601 Imperial Highway; bounded by Esperanza Street, Hawthorn Avenue, Laurel Street, and Descanso Street, Downey, Los Angeles County, CA

  3. 75 FR 22892 - Environmental Impact Statement: Salt Lake County, UT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ...Administration Environmental Impact Statement: Salt Lake County, UT AGENCY: Federal Highway...proposed transportation improvement project in Salt Lake County, Utah. FOR FURTHER INFORMATION...Administration, 2520 West 4700 South, Suite 9A, Salt Lake City, UT 84118, telephone...

  4. 75 FR 9476 - Environmental Impact Statement: Salt Lake County, UT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ...Administration Environmental Impact Statement: Salt Lake County, UT AGENCY: Federal Highway...proposed transportation improvement project in Salt Lake County, Utah. FOR FURTHER INFORMATION...Administration, 2520 West 4700 South, Suite 9A, Salt Lake City, UT 84118, Telephone:...

  5. 76 FR 13017 - Environmental Impact Statement: Los Angeles County, CA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-09

    ... Federal Highway Administration Environmental Impact Statement: Los Angeles County, CA AGENCY: Federal... Environmental Impact Statement will be prepared for a proposed highway project in Los Angeles County, California... District Director, California Department of Transportation, District 7, Division of Environmental...

  6. 22. Historic American Buildings Survey Alameda County Recorder Office Map ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Historic American Buildings Survey Alameda County Recorder Office Map Book 6, page 17 October 1960 SURVEY OF 1868 - Mission San Jose de Guadalupe, Mission & Washington Boulevards, Fremont, Alameda County, CA

  7. 76 FR 31297 - Ravalli County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF AGRICULTURE Forest Service Ravalli County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. ] SUMMARY: The Ravalli County Resource Advisory Committee will meet in Hamilton, Montana....

  8. 76 FR 12691 - Ravalli County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF AGRICULTURE Forest Service Ravalli County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Ravalli County Resource Advisory Committee will meet in Hamilton, Montana....

  9. 75 FR 18482 - Ravalli County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-12

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF AGRICULTURE Forest Service Ravalli County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Ravalli County Resource Advisory Committee will meet in Hamilton, Montana....

  10. 76 FR 21701 - Ravalli County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-18

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF AGRICULTURE Forest Service Ravalli County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Ravalli County Resource Advisory Committee will meet in Hamilton, Montana....

  11. 75 FR 28543 - Ravalli County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-21

    ... From the Federal Register Online via the Government Printing Office ] DEPARTMENT OF AGRICULTURE Forest Service Ravalli County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Ravalli County Resource Advisory Committee will meet in Hamilton, Montana....

  12. 75 FR 14124 - Ravalli County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-24

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF AGRICULTURE Forest Service Ravalli County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Ravalli County Resource Advisory Committee will meet in Hamilton, Montana....

  13. 76 FR 16725 - Ravalli County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF AGRICULTURE Forest Service Ravalli County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Ravalli County Resource Advisory Committee will meet in Hamilton, Montana....

  14. 15. Photocopy from Futhey and Cope's History of Chester County, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Photocopy from Futhey and Cope's History of Chester County, Pennsylvania, 1881, facing page 27 VIEW OF FARM - Joseph Jackson House, Old Baltimore Pike (London Grove Township), West Grove, Chester County, PA

  15. 4. VIEW OF SILVER BRIDGE (ST. MARY'S BRIDGE), CARRYING COUNTY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW OF SILVER BRIDGE (ST. MARY'S BRIDGE), CARRYING COUNTY ROAD OVER SOURIS RIVER NEAR SOUTH END OF REFUGE, LOOKING NORTHEAST - Upper Souris National Wildlife Refuge Dams, Souris River Basin, Foxholm, Ward County, ND

  16. 76 FR 19971 - El Dorado County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ... From the Federal Register Online via the Government Printing Office DEPARTMENT OF AGRICULTURE Forest Service El Dorado County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The El Dorado County Resource Advisory Committee will meet in...

  17. Photocopy of drawing (original in possession of Allegany County, Cumberland, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of drawing (original in possession of Allegany County, Cumberland, MD) CEMENT HOUSE FLOOR PLAN, 1942 - Kelly-Springfield Tire Plant, Cement House, 701 Kelly Road, Cumberland, Allegany County, MD

  18. 44. Photographer unknown Date unknown MENDOCINO COUNTY, SECTION, HIGHWAY 1. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    44. Photographer unknown Date unknown MENDOCINO COUNTY, SECTION, HIGHWAY 1. 1-MEN-1-I #9, MOVING CAMP. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  19. 51. Photographer unknown 1930 HUMBOLDT COUNTY, SECTION A, HIGHWAY 1. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    51. Photographer unknown 1930 HUMBOLDT COUNTY, SECTION A, HIGHWAY 1. 1-HUM-1-A #101, MEASURING BETWEEN TREES, 1930. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  20. 48. Photographer unknown February 1925 MENDOCINO COUNTY, SECTION K, HIGHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    48. Photographer unknown February 1925 MENDOCINO COUNTY, SECTION K, HIGHWAY 1. 1-MEN-1-K #39, LOG GUARD RAIL, 2-25. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  1. 5. TUNNEL TREE AT DRIVETHROUGHTREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. TUNNEL TREE AT DRIVE-THROUGH-TREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NE. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  2. 75 FR 1051 - Christian County Generation, LLC; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-08

    ...Commission [Docket No. EL10-27-000] Christian County Generation, LLC; Notice of Filing December 30, 2009. Take notice that on December 23, 2009, Christian County Generation, LLC pursuant to section 207 of the Federal Energy...

  3. Photocopy of drawing (original In possession of Allegany County, Cumberland, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of drawing (original In possession of Allegany County, Cumberland, MD) WELL WATER RESERVOIR, 1926 - Kelly-Springfield Tire Plant, Cooling Tower, 701 Kelly Road, Cumberland, Allegany County, MD

  4. GENERAL SITE PLAN, HAMILTON AIR FORCE BASE, MARIN COUNTY, CALIFORNIA. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    GENERAL SITE PLAN, HAMILTON AIR FORCE BASE, MARIN COUNTY, CALIFORNIA. Pencil on paper, dated December 4, 1952. Also marked "PWC 103474." By J.Y. Long Company, Engineers, Oakland, California - Hamilton Field, East of Nave Drive, Novato, Marin County, CA

  5. Air pollution and fatal lung disease in three Utah counties

    SciTech Connect

    Archer, V.E. )

    1990-11-01

    A unique situation found in two Utah counties has made it possible to estimate the fraction of respiratory cancer and nonmalignant respiratory disease (NMRD) deaths, which are attributable to community air pollution (CAP) in one county. The two counties were very similar in many ways, including low smoking rates, until a steel mill constructed during WW II caused substantial CAP in one of them. Subsequent differences in mortality rates from both respiratory cancer and NMRD are striking. A third county, similar to many counties outside Utah, was included in the analysis for comparison. In one county, 30-40% of the respiratory cancer and NMRD deaths were attributable to CAP. In this county, NMRD deaths (but not respiratory cancer deaths) were slightly more frequent than in Salt Lake County where smoking rates were twice as high.

  6. View west, barn, east elevation Woods Homestead, Barn, County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View west, barn, east elevation - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  7. View south, barn, north elevation Woods Homestead, Barn, County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View south, barn, north elevation - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  8. View north, barn, south elevation Woods Homestead, Barn, County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View north, barn, south elevation - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  9. View east, barn, west elevation Woods Homestead, Barn, County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View east, barn, west elevation - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  10. The pleistocene geology of Vilas County, Wisconsin

    NASA Astrophysics Data System (ADS)

    Attig, J. W., Jr.

    1984-12-01

    The Wisconsin Valley, Langlade and Ontonagon Lobes of the Laurentide Ice Sheet flowed across all or part of Villas County between 23,000 and 10,000 years ago. These lobes deposited the Wildcat Lake, Nashville and Crab Lake Members of the Cooper Falls formation respectively. Each member is defined by the physical characteristics of a till, and each member also contains supraglacial debris flow sediment, and subglacial and proglacial fluvial sediment. A number of former ice margin positions are indicated in Vilas County by moraines and the ice contact slope of fluvial landforms. Regional correlation shows that the Langlade and Green Bay Lobes remained near their maximum Late Wisconsin extent when the Wisconsin Valley and Chippewa Lobes had retreated up to 100 km. The Ontonagon Lobe advanced into northern Vilas County and deposited the Winegar Moraine, probably just before growth of the Two Creeks Forest Bed in east central Wisconsin.

  11. Selenium status of Utah County residents

    SciTech Connect

    Bown, J.W.; Christensen, M.J.

    1986-03-01

    Counties of low and high soil selenium (Se) content appear to be in close proximity in the state of Utah. The Se status of Utah County residents was evaluated by measurement of plasma Se concentration, and plasma, platelet, and erythrocyte (RBC) Se-glutathione peroxidase (Se-GSH-Px) activity. A Random Digit Dialing procedure was employed to stratify subjects according to sex and annual income (< $10,000, $10-20,000, > $20,000) in a 2 x 3 factorial design, 7 subjects per cell. There were no significant differences due to sex or income. These results suggest (1) the Se status of Utah County residents is similar to that reported for residents of other regions in the US, and (2) no special consideration for income or gender need be made in surveys of population groups to determine Se status.

  12. Energy Efficient Buildings, Salt Lake County, Utah

    SciTech Connect

    Barnett, Kimberly

    2012-04-30

    Executive Summary Salt Lake County's Solar Photovoltaic Project - an unprecedented public/private partnership Salt Lake County is pleased to announce the completion of its unprecedented solar photovoltaic (PV) installation on the Calvin R. Rampton Salt Palace Convention Center. This 1.65 MW installation will be one the largest solar roof top installations in the country and will more than double the current installed solar capacity in the state of Utah. Construction is complete and the system will be operational in May 2012. The County has accomplished this project using a Power Purchase Agreement (PPA) financing model. In a PPA model a third-party solar developer will finance, develop, own, operate, and maintain the solar array. Salt Lake County will lease its roof, and purchase the power from this third-party under a long-term Power Purchase Agreement contract. In fact, this will be one of the first projects in the state of Utah to take advantage of the recent (March 2010) legislation which makes PPA models possible for projects of this type. In addition to utilizing a PPA, this solar project will employ public and private capital, Energy Efficiency and Conservation Block Grants (EECBG), and public/private subsidized bonds that are able to work together efficiently because of the recent stimulus bill. The project also makes use of recent changes to federal tax rules, and the recent re-awakening of private capital markets that make a significant public-private partnership possible. This is an extremely innovative project, and will mark the first time that all of these incentives (EECBG grants, Qualified Energy Conservation Bonds, New Markets tax credits, investment tax credits, public and private funds) have been packaged into one project. All of Salt Lake County's research documents and studies, agreements, and technical information is available to the public. In addition, the County has already shared a variety of information with the public through webinars, site tours, presentations, and written correspondence.

  13. Farmer Adjustments to Drouth In a Texas County.

    E-print Network

    Skrabanek, R. L.; Banks, Vera J.; Bowles, Gladys Kleinwort

    1964-01-01

    by Soil Conservation Service.) Background In formation The climate of Mills County is mild and dry, with extremes in temoerature being. rare. The sum- " Mil/s County' mers are warm and dry. The average summer I Thic county is only a few miles east... and German derivation with approximately 1 percent being of Spanish extraction. The Mills County economy is largely dependent on agriculture. The types of agriculture and changes Figure 5. Typical rolling terrain and scrub vegetation in the range country...

  14. 70. MISSISSIPPI, LOWNDES CO., NORTHWEST CORNER MAP OF LOWNDES COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    70. MISSISSIPPI, LOWNDES CO., NORTHWEST CORNER MAP OF LOWNDES COUNTY, 1931 Detail of ROAD OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C. L. wood, county engineer. Detail of section NW of Columbus (12 mi. N, 9 mi. W), including Plymouth, Waverly, Barton, Buttahatchie R. Orig. scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

  15. 71. MISSISSIPPI, LOWNDES CO. COLUMBUSSTEENS MAP OF LOWNDES COUNTY, 1931 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    71. MISSISSIPPI, LOWNDES CO. COLUMBUS-STEENS MAP OF LOWNDES COUNTY, 1931 Detail of ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C. L. wood, county engineer. Orig. scale: I in. to I mi. Includes Columbus and area NE to steens. Extent: 12 mi. East-West, 9 mi. North-South. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

  16. Selective methanol or formate production during continuous CO? fermentation by the acetogen biocatalysts engineered via integration of synthetic pathways using Tn7-tool.

    PubMed

    Tyurin, Michael; Kiriukhin, Michael

    2013-09-01

    Methanol-resistant mutant acetogen Clostridium sp. MT1424 originally producing only 365 mM acetate from CO?/CO was engineered to eliminate acetate production and spore formation using Cre-lox66/lox71-system to power subsequent methanol production via expressing synthetic methanol dehydrogenase, formaldehyde dehydrogenase and formate dehydrogenase, three copies of each, assembled in cluster and integrated to chromosome using Tn7-based approach. Production of 2.2 M methanol was steady (p < 0.005) in single step fermentations of 20 % CO? + 80 % H? blend (v/v) 25 day runs each in five independent repeats. If the integrated cluster comprised only three copies of formate dehydrogenase the respective recombinants produced 95 mM formate (p < 0.005) under the same conditions. For commercialization, the suggested source of inorganic carbon would be CO? waste of IGCC power plant. Hydrogen may be produced in situ via powered by solar panels electrolysis. PMID:23519429

  17. Day Fire in Ventura County

    NASA Technical Reports Server (NTRS)

    2006-01-01

    [figure removed for brevity, see original site] Annotated Version

    The Day fire has been burning in Ventura County in Southern California since Labor Day, and has consumed more than 160,000 acres. As of September 29, it was 63 percent contained. The Advanced Spaceborne Thermal Emission and Reflection Radiometer on NASA's Terra satellite flew over the fire at 10 p.m. Pacific Time on September 28, and imaged the fire with its infrared camera. The hottest areas of active burning appear as red spots on the image. The blue-green background is a daytime image acquired in June, used as a background to allow firefighters to localize the hot spots.

    With its 14 spectral bands from the visible to the thermal infrared wavelength region, and its high spatial resolution of 15 to 90 meters (about 50 to 300 feet), ASTER images Earth to map and monitor the changing surface of our planet.

    ASTER is one of five Earth-observing instruments launched December 18, 1999, on NASA's Terra satellite. The instrument was built by Japan's Ministry of Economy, Trade and Industry. A joint U.S./Japan science team is responsible for validation and calibration of the instrument and the data products.

    The broad spectral coverage and high spectral resolution of ASTER provides scientists in numerous disciplines with critical information for surface mapping, and monitoring of dynamic conditions and temporal change. Example applications are: monitoring glacial advances and retreats; monitoring potentially active volcanoes; identifying crop stress; determining cloud morphology and physical properties; wetlands evaluation; thermal pollution monitoring; coral reef degradation; surface temperature mapping of soils and geology; and measuring surface heat balance.

    The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission directorate.

    Size: 22.5 by 31.0 kilometers (12.6 by 15.2 miles) Location: 34.6 degrees North latitude, 119.1 degrees West longitude Orientation: North at top Image Data: ASTER Bands 4, 2, and 1 Original Data Resolution: ASTER 15 meters (49.2 feet) and 30 meters (98.4 feet) Dates Acquired: September 28, 2006 and June 19 2006

  18. Bacterial Hypoxic Responses Revealed as Critical Determinants of the Host-Pathogen Outcome by TnSeq Analysis of Staphylococcus aureus Invasive Infection.

    PubMed

    Wilde, Aimee D; Snyder, Daniel J; Putnam, Nicole E; Valentino, Michael D; Hammer, Neal D; Lonergan, Zachery R; Hinger, Scott A; Aysanoa, Esar E; Blanchard, Catlyn; Dunman, Paul M; Wasserman, Gregory A; Chen, John; Shopsin, Bo; Gilmore, Michael S; Skaar, Eric P; Cassat, James E

    2015-12-01

    Staphylococcus aureus is capable of infecting nearly every organ in the human body. In order to infiltrate and thrive in such diverse host tissues, staphylococci must possess remarkable flexibility in both metabolic and virulence programs. To investigate the genetic requirements for bacterial survival during invasive infection, we performed a transposon sequencing (TnSeq) analysis of S. aureus during experimental osteomyelitis. TnSeq identified 65 genes essential for staphylococcal survival in infected bone and an additional 148 mutants with compromised fitness in vivo. Among the loci essential for in vivo survival was SrrAB, a staphylococcal two-component system previously reported to coordinate hypoxic and nitrosative stress responses in vitro. Healthy bone is intrinsically hypoxic, and intravital oxygen monitoring revealed further decreases in skeletal oxygen concentrations upon S. aureus infection. The fitness of an srrAB mutant during osteomyelitis was significantly increased by depletion of neutrophils, suggesting that neutrophils impose hypoxic and/or nitrosative stresses on invading bacteria. To more globally evaluate staphylococcal responses to changing oxygenation, we examined quorum sensing and virulence factor production in staphylococci grown under aerobic or hypoxic conditions. Hypoxic growth resulted in a profound increase in quorum sensing-dependent toxin production, and a concomitant increase in cytotoxicity toward mammalian cells. Moreover, aerobic growth limited quorum sensing and cytotoxicity in an SrrAB-dependent manner, suggesting a mechanism by which S. aureus modulates quorum sensing and toxin production in response to environmental oxygenation. Collectively, our results demonstrate that bacterial hypoxic responses are key determinants of the staphylococcal-host interaction. PMID:26684646

  19. Bacterial Hypoxic Responses Revealed as Critical Determinants of the Host-Pathogen Outcome by TnSeq Analysis of Staphylococcus aureus Invasive Infection

    PubMed Central

    Wilde, Aimee D.; Snyder, Daniel J.; Putnam, Nicole E.; Valentino, Michael D.; Hammer, Neal D.; Lonergan, Zachery R.; Hinger, Scott A.; Aysanoa, Esar E.; Blanchard, Catlyn; Dunman, Paul M.; Wasserman, Gregory A.; Chen, John; Shopsin, Bo; Gilmore, Michael S.; Skaar, Eric P.; Cassat, James E.

    2015-01-01

    Staphylococcus aureus is capable of infecting nearly every organ in the human body. In order to infiltrate and thrive in such diverse host tissues, staphylococci must possess remarkable flexibility in both metabolic and virulence programs. To investigate the genetic requirements for bacterial survival during invasive infection, we performed a transposon sequencing (TnSeq) analysis of S. aureus during experimental osteomyelitis. TnSeq identified 65 genes essential for staphylococcal survival in infected bone and an additional 148 mutants with compromised fitness in vivo. Among the loci essential for in vivo survival was SrrAB, a staphylococcal two-component system previously reported to coordinate hypoxic and nitrosative stress responses in vitro. Healthy bone is intrinsically hypoxic, and intravital oxygen monitoring revealed further decreases in skeletal oxygen concentrations upon S. aureus infection. The fitness of an srrAB mutant during osteomyelitis was significantly increased by depletion of neutrophils, suggesting that neutrophils impose hypoxic and/or nitrosative stresses on invading bacteria. To more globally evaluate staphylococcal responses to changing oxygenation, we examined quorum sensing and virulence factor production in staphylococci grown under aerobic or hypoxic conditions. Hypoxic growth resulted in a profound increase in quorum sensing-dependent toxin production, and a concomitant increase in cytotoxicity toward mammalian cells. Moreover, aerobic growth limited quorum sensing and cytotoxicity in an SrrAB-dependent manner, suggesting a mechanism by which S. aureus modulates quorum sensing and toxin production in response to environmental oxygenation. Collectively, our results demonstrate that bacterial hypoxic responses are key determinants of the staphylococcal-host interaction. PMID:26684646

  20. Functional Analysis of a Bacitracin Resistance Determinant Located on ICECp1, a Novel Tn916-Like Element from a Conjugative Plasmid in Clostridium perfringens.

    PubMed

    Han, Xiaoyan; Du, Xiang-Dang; Southey, Luke; Bulach, Dieter M; Seemann, Torsten; Yan, Xu-Xia; Bannam, Trudi L; Rood, Julian I

    2015-11-01

    Bacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. They act by interfering with the biosynthesis of the bacterial cell wall. In this study, we analyzed an avian necrotic enteritis strain of Clostridium perfringens that was resistant to bacitracin and produced NetB toxin. We identified a bacitracin resistance locus that resembled a bacitracin resistance determinant from Enterococcus faecalis. It contained the structural genes bcrABD and a putative regulatory gene, bcrR. Mutagenesis studies provided evidence that both bcrA and bcrB are essential for bacitracin resistance, and that evidence was supported by the results of experiments in which the introduction of both the bcrA and bcrB genes into a bacitracin-susceptible C. perfringens strain was required to confer bacitracin resistance. The wild-type strain was shown to contain at least three large, putatively conjugative plasmids, and the bcrRABD locus was localized to an 89.7-kb plasmid, pJIR4150. This plasmid was experimentally shown to be conjugative and was sequenced. The sequence revealed that it also carries a tpeL toxin gene and is related to the pCW3 family of conjugative antibiotic resistance and toxin plasmids from C. perfringens. The bcr genes were located on a genetic element, ICECp1, which is related to the Tn916 family of integrative conjugative elements (ICEs). ICECp1 appears to be the first Tn916-like element shown to confer bacitracin resistance. In summary, we identified in a toxin-producing C. perfringens strain a novel mobile bacitracin resistance element which was experimentally shown to be essential for bacitracin resistance and is carried by a putative ICE located on a conjugative plasmid. PMID:26282424

  1. An Archaeological Survey for the Parker County Special Utility District Water System Improvements in Parker County, Texas

    E-print Network

    Moore, William

    2015-07-31

    moved to the county. The county's population and economy continued to expand during most of the late nineteenth century, encouraged by the construction of railroads that linked the area to national markets, attracted newcomers, and led...

  2. 15. 'Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. 'Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919,' showing general plan, plan of top chord, elevation of main girder, transverse section, plan section at deck level. - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA

  3. 18. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919," showing elevation of center pier, elevation and plan of north and south abutments, sections of abutments, pier, and pier footings - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA

  4. 17. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919," showing plan of bars in top flange, elevation of girder reinforcement, plan of bars in bottom flange - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA

  5. A TURKEY NESTING STUDY IN GREGORY COUNTY, SOUTH DAKOTA

    E-print Network

    A TURKEY NESTING STUDY IN GREGORY COUNTY, SOUTH DAKOTA by Tara L. Wertz A thesis submitted Sciences (Wildlife Option) South Dakota State University 1986 #12;A TURKEY NESTING STUDY IN GREGORY COUNTY. of Wildlife and Fisheries Sciences 11 Date Date #12;A TURKEY NESTING STUDY IN GREGORY COUNTY, SOUTH DAKOTA

  6. School Progress Report 2012. Montgomery County Public Schools

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2013

    2013-01-01

    The 2012 School Progress Report for Montgomery County Public Schools (MCPS) provides state, county, and individual school performance data, as well as information on student attendance, high school graduation rates, and the professional qualifications of teachers at the state, district, and school levels. Montgomery County primary schools are

  7. REVIEW PLAN John Redmond Dam Reservoir, Coffee County, Kansas

    E-print Network

    US Army Corps of Engineers

    #12;#12;REVIEW PLAN John Redmond Dam Reservoir, Coffee County, Kansas Reallocation Study Tulsa PLAN John Redmond Dam Reservoir, Coffee County, Kansas Reallocation Study Tulsa District TABLE review for the Final Reallocation Report to the John Redmond Dam and Reservoir, Coffee County, Kansas

  8. 27 CFR 9.231 - Moon Mountain District Sonoma County.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...2014-04-01 2014-04-01 false Moon Mountain District Sonoma County. 9.231 ...Viticultural Areas 9.231 Moon Mountain District Sonoma County. (a) Name...described in this section is Moon Mountain District Sonoma County. For...

  9. 75 FR 68662 - Environmental Impact Statement: St. Louis County, MO

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-08

    ... Federal Highway Administration Environmental Impact Statement: St. Louis County, MO AGENCY: Federal... Peres Boulevard and Lansdowne Avenue in St. Louis County, Missouri. FOR FURTHER INFORMATION CONTACT: Ms... Development Analyst, St. Louis County Department of Highways and Traffic, 121 S. Meramec Avenue,...

  10. 7. Photocopy of map (from Superior Court of Chatham County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photocopy of map (from Superior Court of Chatham County Land Records, Book 2G, p. 299) John McKinnon (County Surveyor), delineator 11 January 1817 PLAT OF SITE, SHOWING EASTERN PORTION OF BUILDING - William Taylor Store, 204 West Bay Street, Savannah, Chatham County, GA

  11. NORTHERN SAN DIEGO COUNTY LAGOONS Historical Ecology Investigation

    E-print Network

    NORTHERN SAN DIEGO COUNTY LAGOONS Historical Ecology Investigation REGIONAL PATTERNS, LOCAL california state University at northridge NORTHERN SAN DIEGO COUNTY LAGOONS Historical Ecology Investigation, Grossinger rm, longcore tr, stein ed, dark sJ, dusterhoff sr. 2014. northern san diego county lagoons

  12. Inpatient Mental Health Services in Metropolitan and Nonmetropolitan Counties.

    ERIC Educational Resources Information Center

    Wagenfeld, Morton O.; And Others

    1988-01-01

    Analyzes the 1983 distribution of inpatient mental health facilities and estimated inpatient beds for all 3,137 U.S. counties categorized on an 18-position urbanization scale. Finds services available in only 13 percent of all nonmetro counties and in virtually none of the most rural, nonadjacent, nonmetro counties. Contains 30 references. (SV)

  13. Hesburgh Libraries and St. Joseph County Public Library partner

    E-print Network

    Pfrender, Michael

    partnership between Notre Dame's main Hesburgh Library and the county library system. Access current bestHesburgh Libraries and St. Joseph County Public Library partner to offer new delivery services. Joseph County Public Library (SJCPL) materials at the Hesburgh Library. Individuals can also obtain

  14. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Red Hill Douglas County... Areas 9.190 Red Hill Douglas County, Oregon. (a) Name. The name of the viticultural area described in this section is Red Hill Douglas County, Oregon. For purposes of part 4 of this chapter, Red...

  15. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Red Hill Douglas County... Areas 9.190 Red Hill Douglas County, Oregon. (a) Name. The name of the viticultural area described in this section is Red Hill Douglas County, Oregon. For purposes of part 4 of this chapter, Red...

  16. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Red Hill Douglas County... Areas 9.190 Red Hill Douglas County, Oregon. (a) Name. The name of the viticultural area described in this section is Red Hill Douglas County, Oregon. For purposes of part 4 of this chapter, Red...

  17. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Red Hill Douglas County... Areas 9.190 Red Hill Douglas County, Oregon. (a) Name. The name of the viticultural area described in this section is Red Hill Douglas County, Oregon. For purposes of part 4 of this chapter, Red...

  18. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Red Hill Douglas County... Areas 9.190 Red Hill Douglas County, Oregon. (a) Name. The name of the viticultural area described in this section is Red Hill Douglas County, Oregon. For purposes of part 4 of this chapter, Red...

  19. 27 CFR 9.169 - Red Hills Lake County.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Red Hills Lake County. 9... Red Hills Lake County. (a) Name. The name of the viticultural area described in this section is Red Hills Lake County. (b) Approved Map. The appropriate maps for determining the boundary of the Red...

  20. 43. Photographer unknown February 1925 MENDOCINO COUNTY, SECTION, HIGHWAY 1. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    43. Photographer unknown February 1925 MENDOCINO COUNTY, SECTION, HIGHWAY 1. 1-MEN-1-1 #12, NEW HIGHWAY DISPLACING OLD COUNTY ROAD, 2-25. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA