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1

Seepage study of the Sevier River and the central Utah, McIntyre, and Leamington canals, Juab and Millard counties, Utah  

USGS Publications Warehouse

(0.25 cubic meter per second) in the Sevier River and about 1.3 cubic feet per second (0.04 cubic meter per second) in the Leamington Canal. It also showed a net loss of about 7 cubic feet per second (0.20 cubic meter per second) in the Central Utah Canal and about 0.8 cubic foot per second (0.02 cubic meter per second) in the McIntyre Canal. The gains in the Sevier River and Leamington Canal probably come chiefly as return seepage of water lost from the Central Utah and McIntyre Canals.

Herbert, L.R.; Cruff, R.W.; Holmes, Walter F.

1981-01-01

2

Extensional collapse along the Sevier Desert reflection, northern Sevier Desert basin, western United States  

NASA Astrophysics Data System (ADS)

Newly released and previously published seismic reflection data from the northern Sevier Desert basin provide a complete seismic transect between the tilted western margin of the basin and the eastern breakaway zone. When tied to well and surface age data, the transect delineates a continuum of extensional fault and basin fill geometries that developed between late Oligocene and Pleistocene time across the basin. A minimum of 18 km of top-to-the-west normal displacement is estimated across the Sevier Desert from only the most conspicuous growth geometries and offsets across listric normal faults that sole downward into the Sevier Desert reflection (SDR). The SDR clearly marks a normal fault zone beneath the entire basin, where stratal truncations are imaged for 50% of the 39 km length of the reflection east of the Cricket Mountains block. Restoration of extensional displacement along this entire 39 km fault length is necessary to reconstruct the pre-Oligocene configuration and erosion level of Sevier thrust sheets across the Sevier Desert area. The SDR normal fault zone underlies the former topographic crest of the Sevier orogenic belt, where it accommodated extensional collapse after cessation of regional contractile tectonism.

Coogan, James C.; Decelles, Peter G.

1996-10-01

3

Testing the Extensional Detachment Paradigm: A Borehole Observatory in the Sevier Desert Basin  

E-print Network

Testing the Extensional Detachment Paradigm: A Borehole Observatory in the Sevier Desert Basin Mountain Resort in the adjacent Wasatch Range), with a one-day field trip to the Sevier Desert basin geology of the Sevier Desert detachment (Canyon Range). Interest in the Sevier Desert detachment (Fig. 3

Christie-Blick, Nicholas

4

Superfund record of decision amendment (EPA Region 4): Wrigley Charcoal Superfund Site, Hickman County, Wrigley, TN, February 2, 1995  

SciTech Connect

This decision document presents the selected Interim Remedial Action (IRA) for the Wrigley Charcoal Site, in Wrigley, Hickman County, Tennessee. The U.S. EPA has modified a wide variety of items that require immediate response action for the first step of cleanup activities to be taken at the Wrigley Charcoal Site. The major goal of these cleanup activities is to address the most serious threats at the Wrigley Charcoal Site by removing contaminated media from the Primary Site flood plain, remediating wastes at the Storage Basin, and through limited access restrictions at the Primary Site and the Storage Basin. The cleanup activities as presented in this IRA Record of Decision (ROD) Amendment will achieve significant risk reduction and will prepare the Site for future remedial activities.

NONE

1995-03-01

5

Tertiary Normal Faulting in the Canyon Range, Eastern Sevier Desert.  

PubMed

The contact between pre-Mesozoic and Tertiary rocks in the western Canyon Range, west-central Utah, has been interpreted as a large, low-angle normal fault that marks the breakaway zone of the hypothesized, basin-forming Sevier Desert detachment. Recent fieldwork suggests that the contact may in fact be depositional along much or all of its length. Deformational fabric in the supposed footwall likely traces to the Mesozoic Sevier orogeny rather than to Tertiary detachment faulting. Kinematic indicators at the range front are not generally consistent with low-angle normal-fault motion; instead, well-exposed high-angle faults are the dominant range-bounding structures. The Tertiary conglomerates of the western Canyon Range foothills, previously viewed as an evolving syntectonic deposit related to detachment faulting, are here reinterpreted as three distinct units that reflect different periods and tectonic settings. The pattern in these conglomerates, and in fault-offset gravity-slide deposits that mantle the western foothills, is consistent with block faulting and rotation along several generations of high-angle structures. Local seismic-reflection data lend qualitative support to this interpretation, and underscore the need to consider alternative working hypotheses for evolution of the Sevier Desert basin. PMID:10517882

Wills; Anders

1999-11-01

6

COMINCO AMERICAN WELL: IMPLICATIONS FOR THE RECONSTRUCTION OF THE SEVIER OROGEN AND BASIN AND  

E-print Network

** ABSTRACT. Re-evaluation of acoustic, gamma ray and dip meter logs from the Cominco American Federal No. 2COMINCO AMERICAN WELL: IMPLICATIONS FOR THE RECONSTRUCTION OF THE SEVIER OROGEN AND BASIN AND RANGE well in the Sevier Desert basin of west-central Utah sheds new light on the interpretation

Christie-Blick, Nicholas

7

Elkmont Vehicle Bridge, Construction PeelAway Great Smoky Mountains National ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Elkmont Vehicle Bridge, Construction Peel-Away - Great Smoky Mountains National Park Roads & Bridges, Elkmont Vehicle Bridge, Spanning Little River at Elkmont Campground, Gatlinburg, Sevier County, TN

8

Anatexis and metamorphism in tectonically thickened continental crust exemplified by the Sevier hinterland, western North America  

NASA Technical Reports Server (NTRS)

This paper presents a thermal and petrologic model of anatexis and metamorphism in regions of crustal thickening exemplified by the Sevier hinterland in western North America, and uses the model to examine the geological and physical processes leading to crustally derived magmatism. The results of numerical experiments show that anatexis was an inevitable end-product of Barrovian metamorphism in the thickened crust of the late Mesozoic Sevier orogenic belt and that the advection of heat across the lithosphere, in the form of mantle-derived mafic magmas, was not required for melting of metasedimentary rocks. It is suggested that, in the Sevier belt, as in other intracontinental orogenic belts, anatexis occurred in the midcrust and not at the base of the crust.

Patino Douce, Alberto E.; Humphreys, Eugene D.; Johnston, A. Dana

1990-01-01

9

New data on the late Pleistocene history of lake fluctuations in the Sevier Desert, Utah  

NASA Astrophysics Data System (ADS)

The chronology of lake-level changes during the late Pleistocene in the Lake Bonneville basin, Utah, is currently understood in a general way. Because the regressive phase of the lake occurred during a critical time in the history of the basin (climate was changing rapidly, humans were moving into suitable habitats that were being made available by the decline of the large lake), it is important to improve the dating of paleohydrologic and paleoclimatic events. We have initiated field and laboratory studies in a part of the Sevier Desert that was studied at a reconnaissance scale 25 to 30 years ago. Our work consists of describing critical stratigraphic sections in natural exposures along the Sevier River and in a core of lake and fluvial sediments, and in dating suitable materials from these sections using radiocarbon and optically stimulated luminescence (OSL). The Sevier basin was integrated with the rest of Lake Bonneville during the time when its highest shorelines were being formed, including during the long period of development of the Provo shoreline (~17.6-14.6 cal ka). The lake regressed rapidly from the Provo shoreline (~1465 m) to the altitude of the divide between the Sevier basin and the Great Salt Lake (GSL) basin (~1390 m), then split into two lakes. In the GSL basin the lake continued to decline as a closed-basin lake, and the lake in the shallow Sevier basin overflowed into it along a large river (the ancestral Sevier River). An extensive wetland, fed by runoff from the Sevier and Beaver Rivers, and by groundwater discharge, developed over much of the Sevier Desert floor during the early Holocene. We have measured new OSL ages that compare favorably with previously obtained radiocarbon ages for the same stratigraphic units. Part of our work is aimed at evaluating the hypothesis that rapid draw-downs in lake level would cause increased groundwater discharge to the lake, and therefore a potential radiocarbon reservoir effect that would have been spatially and temporally heterogeneous.

Oviatt, C. G.; Spencer, J. Q.; Fan, Y.; Leggett, A.

2010-12-01

10

ELSEVIER Tectonophysics 305 (1999) 275286 SevierLaramide deformation of the continental interior from calcite  

E-print Network

from calcite twinning analysis, west-central North America John P. Craddock a,L , Ben A. van der Pluijm by mechanically twinned calcite. Shortening directions are generally parallel to the Sevier thrust as 2000 km into the plate interior. The inferred calcite twinning differential stress magnitudes generally

11

Preliminary Feasibility Assessment of Geologic Carbon Sequestration Potential for TVA's John Sevier and Kingston Power Plants  

SciTech Connect

This is a preliminary assessment of the potential for geologic carbon sequestration for the Tennessee Valley Authority's (TVA) John Sevier and Kingston power plants. The purpose of this assessment is to make a 'first cut' determination of whether there is sufficient potential for geologic carbon sequestration within 200 miles of the plants for TVA and Oak Ridge National Laboratory (ORNL) to proceed with a joint proposal for a larger project with a strong carbon management element. This assessment does not consider alternative technologies for carbon capture, but assumes the existence of a segregated CO{sub 2} stream suitable for sequestration.

Smith, Ellen D [ORNL; Saulsbury, Bo [ORNL

2008-03-01

12

76 FR 31885 - Prevailing Rate Systems; Redefinition of the Northern Mississippi and Memphis, TN, Appropriated...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Systems; Redefinition of the Northern Mississippi and Memphis, TN, Appropriated Fund...geographic boundaries of the Northern Mississippi and Memphis, Tennessee, appropriated...Panola County, MS, from the Northern Mississippi wage area to the Memphis wage...

2011-06-02

13

76 FR 70321 - Prevailing Rate Systems; Redefinition of the Northern Mississippi and Memphis, TN, Appropriated...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Systems; Redefinition of the Northern Mississippi and Memphis, TN, Appropriated Fund...geographic boundaries of the Northern Mississippi and Memphis, Tennessee, appropriated...Panola County, MS, from the Northern Mississippi wage area to the Memphis wage...

2011-11-14

14

1. Elkmont vehicle bridge at Elkmont Campground, galvanized corrugated arch. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Elkmont vehicle bridge at Elkmont Campground, galvanized corrugated arch. - Great Smoky Mountains National Park Roads & Bridges, Elkmont Vehicle Bridge, Spanning Little River at Elkmont Campground, Gatlinburg, Sevier County, TN

15

2. Big Creek Road, worm fence and road at trailhead. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Big Creek Road, worm fence and road at trailhead. - Great Smoky Mountains National Park Roads & Bridges, Big Creek Road, Between State Route 284 & Big Creek Campground, Gatlinburg, Sevier County, TN

16

1. Opposition sign posted on private property at the entrance ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Opposition sign posted on private property at the entrance to the road looking NW. - Great Smoky Mountains National Park Roads & Bridges, Northshore Road, 1 mile spur at Fontana Dam & Bryson City to Noland Creek, Gatlinburg, Sevier County, TN

17

1. View of Cades Cove Valley from first overlook on ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. View of Cades Cove Valley from first overlook on Rich Mountain Road looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

18

10. Reagan's Tub Mill and Roadway looking NE. Great ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Reagan's Tub Mill and Roadway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

19

3. Roaring Fork Motor Nature Trail, view between second and ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

20

7. Roaring Fork Motor Nature Trail, rocks along edge of ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

21

1. Roaring Fork Motor Nature Trail, entrance sign. Great ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

22

6. Roaring Fork Motor Nature Trail, road view after stop ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

23

2. Roaring Fork Motor Nature Trail, road view before first ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

24

9. Roaring Fork Motor Nature Trail, Reagan House. Great ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

25

5. Roaring Fork Motor Nature Trail, vista at stop three. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

26

Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

27

11. Roaring Fork Motor Nature Trail, boulders along road after ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

28

4. Overgrown overlook number 1 looking W. Great Smoky ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Overgrown overlook number 1 looking W. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

29

12. Roaring Fork Motor Nature Trail, place of a thousand ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

30

8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

31

10. Area of landslide on eastern section of Foothills Parkway ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Area of landslide on eastern section of Foothills Parkway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

32

Late Cretaceous extension in the hinterland of the Sevier thrust belt, northwestern Utah and southern Idaho  

SciTech Connect

Cover rocks of the Raft River metamorphic core complex, located in the Sevier belt hinterland, preserve a structural and metamorphic history that predates the middle Tertiary extension of the region. In the eastern Raft River Mountains, Cambrian( )-Permian rocks form two allochthons that occur in the hanging wall of the mid-Miocene Raft River detachment fault. Dramatically attenuated, metamorphosed Cambrian( )-Pennsylvanian strata of the lower allochthon yield Late Cretaceous muscovite {sup 40}Ar/{sup 39}Ar plateau ages (ca. 82-90 Ma) that are interpreted to date cooling during final stages of layer parallel extension. Devonian-Permian rocks exposed in the Black Pine Mountains underwent east-west extension ({approximately} 160%) associated with syntectonic growth of white mica along cleavage. {sup 40}Ar/{sup 39}Ar whole-rock spectra of associated slates also indicate a Late Cretaceous metamorphism. Together, field and {sup 40}Ar/{sup 39}Ar results suggest that Late Cretaceous extension occurred in the Sevier belt hinterland at the same time as shortening in the eastern foreland and at depth in the hinterland. Sufficient topography must have been present to drive upper-crustal extension in the eastern hinterland.

Wells, M.L.; Allmendinger, R.W. (Cornell Univ., Ithaca, NY (USA)); Dallmeyer, R.D. (Univ. of Georgia, Athens (USA))

1990-10-01

33

Cretaceous crustal structure and metamorphism in the hinterland of the Sevier thrust belt, western US Cordillera  

SciTech Connect

Combined structural and geochronologic studies to the west of the Sevier belt at lat 39{degree}-40{degree}N indicate that peak metamorphism of the upper 15 km of crust occurred during the Late Cretaceous (70-90 Ma), overlapping in time with the intrusion of muscovite-bearing granites and pegmatites. Metamorphism was accompanied by penetrative deformation (top-to-the-east layer-parallel shear); both were localized at the currently exposed structural levels, resulting in impressive vertical and lateral strain gradients and T{sub max} gradients of > 50 C/km. Inception of thrust faulting in the Sevier belt may have predated peak metamorphism and intrusion of granites in the hinterland by 10-15 m.y., but it continued during and ended simultaneously with the end of metamorphism at about 70 Ma. The rise of muscovite-bearing granites and hot fluids, generated by metamorphism and crustal anatexis at greater depth, provides and explanation for the observed localized metamorphism and deformation at relatively high structural levels.

Miller, E.L.; Gans, P.B. (Stanford Univ., CA (USA))

1989-01-01

34

Sevier Laramide deformation of the continental interior from calcite twinning analysis, west-central North America  

NASA Astrophysics Data System (ADS)

Paleozoic-Mesozoic carbonates that cover cratonic western North America contain a regional layer-parallel shortening (LPS) fabric that is preserved by mechanically twinned calcite. Shortening directions are generally parallel to the Sevier thrust-transport direction (E-W) in carbonates of the Idaho-Wyoming portion of the thrust belt and within carbonates as far as 2000 km into the plate interior. The inferred calcite twinning differential stress magnitudes generally decrease across the thrust belt, and decrease exponentially away from the orogenic front into the craton. Synorogenic calcite cements and veins preserve a distinct twinning deformation history: in the thrust belt, twinning strains commonly record local, out-of-transport piggyback strain events with high differential stresses (<150 MPa), whereas in Laramide uplifts and adjacent basins as far east as the Black Hills, twinned vein calcite preserves a sub-horizontal, N-S-shortening strain, with differential stress magnitudes that decrease to the east. Deformation of the plate interior during the Sevier orogeny was dominated by E-W contraction at the plate margin, which changed into dominantly oblique contraction (˜N-S shortening) along western North America during the younger, basement-involved Laramide event.

Craddock, John P.; van der Pluijm, Ben A.

1999-05-01

35

Relationship of Cretaceous synorogenic conglomerate in central Utah to Sevier thrust events  

SciTech Connect

A boulder conglomerate facies is found almost continuously along the western edge of the Cretaceous seaway in central and southern Utah. At the present time, north-south continuity of the facies is disrupted by Tertiary volcanic cover and basin and range-type faulting, making the original distribution of conglomerate difficult to follow. Careful physical comparison of good conglomerate exposures and developing palynologic control in fine-grained equivalent sediments to the east allows some correlation of the facies trend and indicates a close time relationship with Sevier thrust events. Silicic and carbonate boulder conglomerates were limited to a relatively narrow east-west zone directly in front of the leading thrust edge. Rapid eastward transition to well-sorted sand and coastal marine conditions suggests deposition relatively close to base level. Major unconformities within the conglomerate sequence and the upper and lower limits of coarse clastic sedimentation define several depositional episodes. It appears that the conglomerate packages are related to specific Sevier orogenic events.

Pratt, R.M.

1985-05-01

36

Stratigraphy and structure of the Sevier thrust belt and proximal foreland-basin system in central Utah: A transect from the Sevier Desert to the Wasatch Plateau  

USGS Publications Warehouse

The Sevier orogenic belt in central Utah comprises four north-northwest trending thrust plates and two structural culminations that record crustal shortening and uplift in late Mesozoic and early Tertiary time. Synorogenic clastic rocks, mostly conglomerate and sandstone, exposed within the thrust belt were deposited in wedge-top and foredeep depozones within the proximal part of the foreland-basin system. The geologic relations preserved between thrust structures and synorogenic deposits demonstrate a foreland-breaking sequence of thrust deformation that was modified by minor out-of-sequence thrust displacement. Structural culminations in the interior part of the thrust belt deformed and uplifted some of the thrust sheets following their emplacement. Strata in the foreland basin indicate that the thrust sheets of central Utah were emplaced between latest Jurassic and Eocene time. The oldest strata of the foredeep depozone (Cedar Mountain Formation) are Neocomian and were derived from the hanging wall of the Canyon Range thrust. The foredeep depozone subsided most rapidly during Albian through Santonian or early Campanian time and accumulated about 2.5 km of conglomeratic strata (Indianola Group). The overlying North Horn Formation accumulated in a wedge-top basin from the Campanian to the Eocene and records propagation of the Gunnison thrust beneath the former foredeep. The Canyon Range Conglomerate of the Canyon Mountains, equivalent to the Indianola Group and the North Horn Formation, was deposited exclusively in a wedge-top setting on the Canyon Range and Pavant thrust sheets. This field trip, a three day, west-to-east traverse of the Sevier orogenic belt in central Utah, visits localities where timing of thrust structures is demonstrated by geometry of cross-cutting relations, growth strata associated with faults and folds, or deformation of foredeep deposits. Stops in the Canyon Mountains emphasize geometry of late structural culminations and relationships of the Canyon Range thrust to growth strata deposited in the wedge-top depozone. Stops in the San Pitch Mountains illustrate deposits of the foredeep depozone and younger, superjacent wedge-top depozone. Stops in the Sanpete Valley and western part of the Wasatch Plateau examine the evolution of the foreland-basin system from foredeep to wedge-top during growth of a triangle zone near the front of the Gunnison thrust.

Lawton, T.F.; Sprinkel, D.A.; Decelles, P.G.; Mitra, G.; Sussman, A.J.; Weiss, M.P.

1997-01-01

37

The Cave Canyon Detachment: A Standard for Deformation Expected of the Sevier Desert Detachment  

NASA Astrophysics Data System (ADS)

The Cave Canyon detachment (CCd)is a Neogene normal fault in the Mineral Mountains of west-central Utah. The Mineral Mountains are located directly south of the Sevier Desert basin. Because of its location, orientation and age constraints the Cave Canyon detachment has been proposed to be a non-active splay of the Sevier Desert detachment. The Sevier Desert detachment (SDd) is thought to be one of the largest displaced active normal faults in North America accruing as much as 47 km of displacement. There are some fundamental differences between the CCd and the SDd, one of those being that the CCd places Paleozoic limestone directly on a granitic pluton whereas the SDd beneath the basin places Neogene terrestrial sediments directly on Paleozoic limestones. Nevertheless, the temperature and depth at the time of movement are similar, thus making the CCd an appropriate analog to what might be expected of material potentially recovered by scientific drilling of the SDd. A suite of samples from above and below the CCd yields a distinctive pattern of brittle deformation. In the lower plate granites deformation is mostly brittle, and in the upper plate there is only a minor zone a few cm thick of mylonitized carbonate. The lower plate granites are characterized by an aureole of healed microfractures that decrease in abundance from the contact to background at over 100 m. Cataclasis is observed well over 100 m from the detachment, and is so intense at the contact that no grain is unaffected. Significant growth of new minerals and cross-cutting quartz veins are observed within a few meters of the detachment. In upper plate carbonate rocks, a cm-thick zone of dynamic recrystallization is observed, above which there is a meter or so of extensive undulose extinction of grains. Outward from this is a zone of strongly preferred oriented twinning. At a distance of 15 m from the contact only minor carbonate veinlets distinguish fault zone rock from unaffected limestone. If the SDd is an active or recently active fault zone with displacement as much as an order of magnitude greater than that of the CCd, and appreciably more deformation would be expected within the fault zone both above and below the detachment surface.

Anders, M. H.

2007-12-01

38

Ages of Sevier thrusting from dating of metamorphic garnet using the Lu-Hf method  

NASA Astrophysics Data System (ADS)

Combined thermodynamic modeling of garnet growth zoning and Lu-Hf dating of garnet yield well-constrained pressure-temperature-time (PTt) paths. Here we present PTt paths from amphibolite-facies pelitic garnet from the Raft River-Albion-Grouse Creek metamorphic core complex that constrain the timing of thrusting in the hinterland of the Sevier thrust belt. Three general times of thrust burial are indicated: 150, 138, and 85 Ma. Lu-Hf garnet dating of burial-related garnet growth in the Raft River Mountains yielded a Late Jurassic age of 149.9 ± 1.2 Ma (2?, MSWD = 1.1) based on three garnet fractions and a whole rock. A PT path from the schist of Mahogany Peaks in the Albion Range, Idaho, records an isothermal pressure increase indicating growth during thrusting. Lu-Hf dating of garnet from the same rock yielded an Early Cretaceous age of 138.7 ± 0.7 Ma (2?, MSWD = 1.6) based on seven garnet fractions. An additional PT path from a nearby outcrop also records an isothermal pressure increase and a similar Lu-Hf garnet age of 132.1 ± 5.1 Ma (2?, MSWD = 9.5) based on three garnet fractions and a whole rock. PT paths of multiple garnet grains from the schist of Stevens Spring in the Grouse Creek Mountains, Utah, exhibit isothermal pressure increases and yielded a Lu-Hf garnet age of 85.5 ± 1.9 Ma (2?, MSWD = 3.9) based on five garnet fractions and a whole rock. The Late Jurassic burial event recorded in the Raft River Mountains is older than the ages of inception of thrusting of the western thrusts of the Sevier fold-thrust belt including the Canyon Range and Paris - Willard thrusts, but consistent with an eastward progression in initial shortening in the orogenic wedge and development of an inferred thrust load responsible for the retroarc Morrison Formation basin. Early Cretaceous hinterland burial recorded in the Albion Range is permissively coeval with activity on the Willard and Canyon Range thrusts. Finally, renewed hinterland thrust burial during the Late Cretaceous, as documented in the Grouse Creek Mountains, is consistent in timing with previous interpretations of major thrusting in frontal thrust systems of the Sevier belt, such as the development of the Absaroka thrust.

Cruz-Uribe, A. M.; Hoisch, T. D.; Wells, M. L.; Vervoort, J. D.

2008-12-01

39

Upper Oligocene evaporites in basin fill of Sevier Desert region, western Utah ( USA).  

USGS Publications Warehouse

The basin fill beneath the Sevier Desert of western Utah contains evaporites that were deposited in a broad closed basin. All of the basin fill penetrated by the Gulf Oil 1 Gronning contains abundant volcanic detritus and its alteration products of Cenozoic age. Fission-track dating of tuffaceous sandstone yields ages of 26-28 m.y. Fossil pollen from mudstone in the evaporite-bearing strata includes forms no older than late Oligocene, in good agreement with the fission-track ages. Thus the age of evaporites is late Oligocene and younger. Anhydrite is present throughout approximately 900m of volcaniclastic rocks in the lower part of the section cut by the Gulf hole. Fossil pollen are indicative of an arid to semiarid flora.-from Authors

Lindsey, D.A.; Glanzman, R.K.; Naeser, C.W.; Nicholas, D.J.

1981-01-01

40

Role of crustal thickening and extensional collapse in the tectonic evolution of the Sevier-Laramide orogeny, western United States  

SciTech Connect

The effects of crustal thickening and extensional collapse of continental crust, combined with variations in plate-convergence forces, provide a coherent explanation for evolution of the Cretaceous-early Tertiary Sevier-Laramide orogeny. Crustal thickening of the Sevier-Laramide hinterland region was triggered by the combination of compressive plate-convergence forces and subduction-induced conductive heating of the crust. Eastward progradation of the locus of Sevier-Laramide deformation through time reflects the progressive widening of the region of crustal thickening. The region of maximum crustal thickening migrated outward until about 80 to 75 Ma, when it encountered excessively strong lithosphere of the Colorado Plateau. The locus of deformation was then transmitted laterally across both a hinterland that had attained maximum crustal thickness and the rigid Colorado Plateau, into the Laramide Rocky Mountain foreland. An episode of vigorous extensional collapse of orogenically thickened crust affected the southern Cordillera between about 75 and 35 Ma. Early Tertiary waning of plate-convergence rates and continued subduction-induced conductive heating of thickened crust allowed extensional collapse along the southern Cordillera region to drive the Colorado Plateau block northward. The result was the late Laramide phase of Rocky Mountain foreland deformation in the central and southern Laramide Rocky Mountain region continued through Eocene time, rather than ceasing at 56 Ma, as it did in the northern Cordillera.

Livaccari, R.F. (Univ. of New Mexico, Albuquerque (United States))

1991-11-01

41

3. BIG HOUSE (left) AND CORN CRIB (right) IN THE ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. BIG HOUSE (left) AND CORN CRIB (right) IN THE BACKGROUND. See also individual HABS documentation: Walker Family Farm, Big House (HABS No. TN-121 A), and Walker Family Farm, Corn Crib (HABS No. TN-121 C). - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

42

Testing the Extensional Detachment Paradigm: A Borehole Observatory in the Sevier Desert Basin, Utah  

NASA Astrophysics Data System (ADS)

The Sevier Desert basin, Utah represents a world-class target for scientific drilling and for the development of an in situ borehole observatory of active faulting, with potential for establishing that normal-sense slip can occur along a brittle low-angle fault and, by determining the conditions under which that may take place, for resolving the mechanical paradox associated with such structures. The Sevier Desert detachment was defined in the mid- 1970s on the basis seismic reflection data and commercial wells as the contact between Paleozoic carbonate rocks and Cenozoic basin fill over a depth range of ~0-4 km. Today, the interpreted fault is thought by most workers to root into the crust to the west, to have large estimated offset (< 47 km), to have been active over most of its history near its present 11° dip, and to be associated with contemporary surface extension (a 30- km-long zone of prominent Holocene fault scarps immediately west of Clear Lake). Although no seismicity has been documented on the detachment, its scale is consistent with earthquake magnitudes as large as M 7.0. A published alternative interpretation of the Paleozoic-Cenozoic contact as an unconformity rather than a fault has not been generally accepted. Deformation at detachment faults is commonly spatially restricted, and may have been missed in well cuttings. Exhumation of the detachment would have made it possible to remove critical footwall evidence prior to later sedimentary onlap, particularly at updip locations. The incomplete coverage and uneven quality of seismic reflection data on which the detachment interpretation depends, and an unresolved debate about stratigraphic ties to a critical well, leave room for discussion about interpretive details, including the possibility that deformation was distributed across several closely spaced faults. An apparent mismatch between stratigraphically based ages and fission-track evidence for the timing of footwall exhumation cannot be resolved with available well data. Drilling is now needed to make in situ measurements at depth, to obtain critical core of fault rocks at a down-dip site where offset should be large, and to establish more clearly the relationship between basin development and displacement along the interpreted fault. A workshop will take place from July 15-18, 2008, in Utah, under the auspices of the International Continental Scientific Drilling Program, to flesh out objectives, strategies and operational details, and to develop a consensus on the location of a drill site.

Christie-Blick, N.; Wernicke, B. P.

2007-12-01

43

Three-dimensional digital-computer model of the principal ground-water reservoir of the Sevier Desert, Utah  

USGS Publications Warehouse

The principal ground-water reservoir in part of the Sevier Desert, Utah was modeled using a three-dimensional finite-difference model. The model was calibrated by comparing computed and observed water-level measurements in 1952, simulating results of aquifer tests, and simulating ground-water withdrawals during 1952-81 and comparing computed water-level changes during 1952-82 to observed changes. The results indicate the model is capable of predicting future water-level changes. The report includes a complete listing of the model data for the 1952-82 simulation. (USGS)

Holmes, W.F.

1983-01-01

44

Upper Oligocene evaporites in basin fill of Sevier Desert region, western Utah  

SciTech Connect

The basin fill beneath the Sevier Desert of western Utah contains evaporites of late Oligocene age that were deposited in a broad closed basin. All of the basin fill penetrated by the Gulf Oil 1 Gronning (2458 m TD), one of only three deep holes in the basin, contains abundant volcanic detritus and its alteration products of Cenozoic age, when volcanism was intense in western Utah. Fission-track dating of tuffaceous sandstone, interbedded with evaporite minerals and representative of sandstone in the lower formations penetrated, yields ages of 26 to 28 m.y.; concordance of ages indicates no thermal resetting. Fossil pollen from mudstone in the evaporite-bearing strata includes forms no older than late Oligocene, in good agreement with the fission-track ages. Thus the age of the evaporites, and of all the basin fill penetrated by drilling, is late Oligocene and younger; previous work had assigned them ages ranging from Triassic to Eocene. Evaporite-bearing rocks were deposited during late Oligocene time in a broad closed basin under arid or semiarid conditions much like those of the Great Basin today. Anhydrite is present throughout approximately 900 m of volcaniclastic rocks in the lower part of the section cut by the Gulf hole, and more than 1500 m of anhydrite and halite is present in possibly equivalent strata in the nearby Argonaut dry hole. Fossil pollen from anhydrite-bearing rocks in the Gulf hole are indicative of an arid to semiarid flora, including plants similar to Mormon tea and possibly saltbush or buffalo berry. Evaporite minerals formed in the basin during dry periods; tuff was erupted from nearby volcanic centers, reworked by water, and deposited in the basin.

Lindsey, D.A. (Geological Survey, Denver, CO); Glanzman, R.K.; Naeser, C.W.; Nichols, D.J.

1981-02-01

45

Spatial and Temporal Distribution of Strain in the Sevier Desert Region from a Decade of BARGEN Continuous GPS Observations  

NASA Astrophysics Data System (ADS)

A transect of four BARGEN GPS sites from 1997-2000 at latitude 39°N revealed a linear increase in velocity from 0 mm/yr on the Colorado Plateau to ~4 mm/yr in east-central Nevada. These geodetic data, when combined with paleoseismic and neotectonic observations, were used to argue for present-day strain accumulation on the Sevier Desert detachment, a seismically-imaged, low-angle (12°) normal fault that underlies a significant portion of west central Utah [Niemi et al., 2004]. A new GPS velocity solution [Davis et al., 2006], including 5 additional years of observations and a new GPS station in the eastern half of the transect, serves to both clarify and complicate our understanding of the spatial and temporal distribution of strain in this region. Geodetic velocity gradients among the three original eastern sites (from east to west, CAST, SMEL, and FOOT), and including the new site, SPIC, located between CAST and SMEL, continue to suggest a linear strain gradient from the Colorado Plateau to westernmost Utah, on a transect spanning the Wasatch, Sevier Desert, and House Range normal faults. In contrast, the baseline between FOOT and EGAN, which underwent extension from 1997-2000, began to contract in 2000, as site EGAN slowed with respect to the Colorado Plateau. This deviation in velocity continued until 2003, when EGAN began moving westward, and has, as of 2006, returned to near its pre-2000 velocity with respect to the Colorado Plateau. The eastward excursion of GPS site EGAN is not unique, and similar excursions are observed in the time series of all BARGEN GPS sites that lie west of ~114.25°W over the time period 2000-2003 [Davis et al., 2006]. The origin of this velocity anomaly is uncertain, but the magnitude and spatial extent of the excursion, as well as the observation of a deep earthquake swarm (~30-40 km depth) coincident with dramatic motion of GPS site SLID, near Lake Tahoe, in 2003 [Smith et al., 2004] suggest a deep crustal or mantle influence on the observed GPS velocities. Bright, mid-crustal horizontal reflections observed in COCORP reflection seismic data west of 114°W have been postulated to represent a detachment that could accommodate shearing along the base of the crust in Nevada [Hauser et al., 1987], a hypothesis broadly consistent with the observed GPS velocities, with the crust shearing relatively east over the subcrustal lithosphere. A deep observatory in the Sevier Desert region, transecting the Sevier Desert detachment, would complement existing paleoseismic and neotectonic studies, and the decade of BARGEN continuous GPS observations, as well as new GPS data from 8 Earthscope Plate Boundary Observatory sites installed along this transect in the past few years. Key questions that could be assessed with a deep observatory are how slip, and strain, at depth on an inclined fault are reflected in geodetic observations of strain at the surface; whether the state of stress and strain rate on the Sevier Desert detachment change through time, as suggested by long-term periodicity in strain release as recorded in the geologic record, and what effect long-lived velocity excursions, such as observed from 2000-2003, have on the regional stress state, perhaps leading to a clearer understanding of the source of these anomalies, and the aseismic tectonic behavior of the lithosphere.

Niemi, N. A.; Wernicke, B. P.

2007-12-01

46

Learning to See a Brighter Future for Morgan County, TN  

Microsoft Academic Search

There is a critical need to develop land planning processes that can build the capacities of local communities to address stewardship and sustainability at both the individual and collective\\/landscape scales. Social learning has been advocated as a process by which to build the capacity of local communities to address these issues. This paper outlines a social learning process currently being

Jonah Fogel

47

Structural styles of the Guess Creek fault block beneath the Great Smoky thrust sheet, Blount County, Tennessee  

SciTech Connect

A road cut along US 321 N, approximately 1 km NW of Walland, TN, exposes a previously unexposed complexly deformed section of Middle Ordovician clastic wedge [Chickamauga Group, Sevier Shale] sedimentary rocks. It provides an excellent opportunity to analyze both the lithologic assemblages and complex folding and faulting beneath the Great Smoky thrust sheet. Arkosic quartzite of the Lower Cambrian Cochran Conglomerate [Chilhowee Group], has been thrust over weaker Sevier Shale in the hanging wall of the Guess Creek fault. Regionally, the Great Smoky fault separates metamorphosed Precambrian to Lower Cambrian clastic shelf, slope, and rift facies rocks of the western Blue Ridge from Cambro-Ordovician carbonate shelf and orogenic wedge deposits of the foreland fold and thrust belt. West of the Great Smoky fault, the Guess Creek fault has been interpreted to floor duplexed Cambro-Ordovician rocks exposed in windows beneath the Great Smoky thrust sheet in the vicinity of the Great Smoky Mountains National Park. The Sevier Shale here consists of variably cleaved shale, siltstone, sandstone, and conglomerate. It exhibits a variety of fold styles throughout the exposure, ranging from predominantly noncylindrical tight folds to broad, open structures. A weak axial-planar pencil cleavage is developed in the Middle Ordovician shale and siltstone, along with a secondary cleavage that transects the axial surfaces of the folds. Minor thrust faults within the Sevier Shale appear to have formed by propagation through tightened fold hinges or bedding-parallel slip. The fold pattern observed in the roadcut appears to be partly the result of movement along a tear fault that broke both the hanging wall and footwall of the Great Smoky thrust sheet after emplacement. Slickenline orientations along minor thrust surfaces in the Cochran Conglomerate indicate eastward-directed, oblique-slip movement of the tear fault.

Carter, M.W.; Davidson, G.L.; Heller, J.A. (Univ. of Tennessee, Knoxville, TN (United States). Dept. of Geological Sciences); Hatcher, R.D. Jr. (Oak Ridge National Lab., TN (United States))

1993-03-01

48

Cretaceous to early Tertiary paleogeography in the hinterland of the Sevier thrust belt, east-central Nevada  

SciTech Connect

The Cretaceous to early Tertiary hinterland of the Sevier thrust belt in east-central Nevada underwent two periods of sedimentary basin development. The older (Early Cretaceous) episode is characterized by basins that formed coeval with deformation within a belt of contractional structures. Basins were characterized by periods of through-flowing fluvial drainage. The younger (Late Cretaceous to Eocene) episode of basin development was characterized by internally drained alluvial basins that subsequently expanded into more regional lacustrine basins. Basal alluvial detritus indicates that basin inception occurred as a result of rapid development of topographic relief. Synsedimentary tectonism was characterized by horizontal extension due to attainment of equilibrium topography in response to crustal thickening.

Vandervoort, D.S.; Schmitt, J.G. (Montana State Univ., Bozeman (USA))

1990-06-01

49

Mucin associated Tn and sialosyl-Tn antigen expression in colorectal polyps  

Microsoft Academic Search

Sialosyl-Tn antigen and its immediate precursor, Tn antigen, are carbohydrate structures associated with the earliest steps of mucin O-linked glycosylation. Both antigens have been shown previously to be highly sensitive and specific markers of colorectal cancer. One hundred and three colorectal polyps (79 adenomatous; 24 hyperplastic) were examined for expression of Tn antigen using vicia villosa isolectin B4, and for

S H Itzkowitz; E J Bloom; T S Lau; Y S Kim

1992-01-01

50

Laserprobe 40Ar/39Ar Dating Of Strain Fringes: An Example From The Interior Of The Sevier Orogen  

NASA Astrophysics Data System (ADS)

Determining the absolute ages of deformation fabrics in metamorphic rocks is critical to understanding the nature of orogenesis in deeply exhumed ancient mountain belts. In situ isotopic analysis of fibrous strain fringes is particularly advantageous because the relationships between the analyzed mineral, deformation fabric, and isotopic age can be established, and mineral growth can be linked to the progressive strain history. Furthermore, when isotopic closure temperatures (Tc) are higher than crystallization temperatures, isotopic ages reflect deformation ages. We have applied in situ 40Ar/39Ar UV and CO2 laser techniques to dating phlogopite in quartz-phlogopite-calcite and muscovite in quartz-muscovite fibrous strain fringes in greenschist-facies metamorphic rocks from the Grouse Creek Mountains (Utah) and Black Pine Mountains (Idaho). In both cases, syntectonic mica growth occurred below the minerals Tc. Phlogopite 40Ar/39Ar ages for individual strain fringes in the Grouse Creek Mountains range from 92 Ma to 110 Ma, with the most reliable ages ranging from 101 Ma to 110 Ma (mean age, 105.0 plus or minus 5.8 Ma). Muscovite 40Ar/39Ar ages for individual strain fringes in the Black Pine Mountains range from 97 Ma to 112 Ma (mean age, 104.7 plus or minus 5.8 Ma). Strain fringes are associated with a sub-horizontal foliation and a generally N-trending elongation lineation exhibiting components of top-to-the-north simple shear and coaxial strain accommodating N-S extension and sub-vertical shortening. The new dates confirm prior correlation of fabrics between these ranges and yield a mid-Cretaceous age for the earliest ductile fabric preserved in the Raft River-Albion-Grouse Creek metamorphic core complex, in the interior (hinterland) of the retroarc Sevier orogenic belt. Mid-crustal northward flow at 105 (plus or minus 6) Ma within the interior of the Sevier orogen, coeval with east-directed shortening in the foreland and with plate convergence, records orogen-parallel synconvergent extension. Gravitational relaxation of structural culminations resulting from focused crustal shortening may be the driving mechanism for orogen-parallel flow.

Wells, M. L.; Spell, T. L.; Hoisch, T. D.; Arriola, T.; Zanetti, K. A.

2008-12-01

51

Genetic and molecular studies of a composite chromosomal element (Tn3705) containing a Tn916-modified structure (Tn3704) in Streptococcus anginosus F22.  

PubMed

The plasmid-free Streptococcus anginosus F22 contained a conjugative element, Tn3705, encoding resistance to erythromycin (Emr) and tetracycline-minocycline (Tcr-Mnr). We mapped a chromosomal region (> 52 kb) of F22, corresponding to the internal part of Tn3705. Molecular analysis of Tn3705 revealed it to be a composite structure: it included in its central part a transposon designated Tn3704 (20.3 kb +/- 0.5 kb), which had a modified structure in comparison with that of Tn916 and on which the Emr Tcr-Mn4 markers were localized. Tn3705 inserted from F22 into the chromosome of various streptococcal transconjugants as well as that of Enterococcus faecalis transconjugants without changing its structure. In contrast, from the chromosome of an E. faecalis::Tn3705 transconjugant only Tn3704 inserted, at various sites, into another E. faecalis chromosome. Sugar fermentations occurred after the insertion of Tn3704 into the chromosome of an asaccharolytic E. faecalis strain. Transposition of only Tn3704 from the chromosome of E. faecalis::Tn3705 onto pIP964, an E. faecalis hemolysin plasmid, yielded two different pIP964 derivatives. The size of the entire element Tn3705 was estimated to be about 70.0 kb by pulsed-field electrophoresis. PMID:8171124

Clermont, D; Horaud, T

1994-01-01

52

Tn10 mutagenesis in Azotobacter vinelandii.  

PubMed

The tetracycline-resistant transposon Tn10 and its "high-hopper" derivative Tn10HH104 were introduced into the Azotobacter vinelandii genome using suicide conjugative plasmids derived from pRK2013. Several types of mutants induced by either of these elements are described. Nif- mutants (deficient in nitrogen fixation) were easily isolated, whereas the isolation of other mutant types (auxotrophs, sugar non-users) required special selection conditions. The characterization of the mutations as transposon insertions was often complicated and sometimes required a combination of genetic and physical tests. A common source of complication, the existence of double inserts, was found among the mutants induced by Tn10HH104 but not among those induced by Tn10. Both the high-hopper and the wild-type element proved to undergo secondary transpositions, albeit at different frequencies. Another type of complication, the existence of heterozygotes, occurred because of the high level of redundancy of the A. vinelandii genome. PMID:17191343

Contreras, A; Casadesús, J

1987-09-01

53

Paleogeographic and paleotectonic setting of sedimentary basins in the Sevier thrust belt and hinterland, eastern Great Basin  

SciTech Connect

The eastern Great Basin contains a sparse record of broadly distributed Cretaceous sedimentary rocks which record: evolution of intermontane basins during development of the Sevier (Sv)contractional orogen and incipient extensional collapse of the elevated Sv hinterland (east-central NV), and complex tectono-sedimentary interactions between frontal thrust belt structures and the western margin of the adjacent foreland basin. Palinspastic restoration of these strata and associated structures to pre-Tertiary extension positions reveals a clearer pictures of Cretaceous basin paleogeography and allows comparison with the Puna/Altiplano plateau and precordillera thrust belt of the Neogene Andean orogen. Two syntectonic stratal assemblages are present in east-central NV. Lower Cretaceous alluvial strata (Newark Canyon Fm) record basin development coeval with emergence of contractional structures in the Sv hinterland. Localized early Cretaceous basins were possibly piggyback immature; periods of open drainage to the to the east and south suggest connection with the nascent Sv foreland basin to the east (Cedar Mountain/Sanpete Fms) prior to major thrust loading in central Utah. Development of hinterland structures is almost recorded by Aptian-Albian foreland basin alluvial deposits in SW Utah (Dakota Fm) and southern Nevada (Willow Tank Fm). Upper Cretaceous to Eocene strata (Sheep Pass Fm) record inception of regionally abundant alluvial-lacustrine basins which developed in response to onset of latest Cretaceous extension and associated collapse of the Sv hinterland. Evolution of the structurally complex western margin of the Sv foreland basin is recorded in Cretaceous through Eocene strata deposited in: piggyback basins which were at times hydrologically connected to the adjacent foreland basins, and thrust-proximal portions of the foreland basin. These proximal areas are characterized by folding and faulting of basin fill and development of intrabasinal unconformities.

Schmitt, J.G. (Montana State Univ., Bozeman, MT (United States). Dept. of Earth Sciences); Vandervoort, D.S. (Cornell Univ., Ithaca, NY (United States). Dept. of Geological Sciences); Suydam, J.D. (Washington State Univ., Pullman, WA (United States). Dept. of Geology)

1993-04-01

54

77 FR 13195 - Amendment of Class E Airspace; Springfield, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...in the Springfield, TN area. Aydelotte Airport has been abandoned and controlled airspace is no longer needed. Airspace reconfiguration...surface at Springfield, TN, as the Aydelotte Airport has been abandoned and is being removed from the airspace description....

2012-03-06

55

Characterization of the staphylococcal beta-lactamase transposon Tn552.  

PubMed Central

The staphylococcal beta-lactamase transposon Tn552 is a member of a novel group of transposable elements. The organization of genes in Tn552 resembles that of members of the Tn21 sub-group of Tn3 family transposons, which transpose replicatively by cointegrate formation and resolution. Thus, a possible resolution site ('resL') and a resolvase gene (tnpR or 'binL') have been identified. However, consistent with the fact that Tn552 generates 6 bp (rather than 5 bp) flanking direct repeats of target DNA, neither the putative transposase protein, nor the terminal inverted repeats of Tn552 are homologous to those of Tn3 elements. Tn552, like phage Mu and retroelements, is defined by the terminal dinucleotides 5' TG .. CA 3'. A naturally occurring staphylococcal plasmid, pI9789, contains a Tn552-derived resolution system ('resR-binR') that acts as a 'hotspot' for Tn552 transposition; insertion creates a segment of DNA flanked by inversely repeated resolution sites, one (resR) on pI9789 and the other (resL) on Tn552. The putative Tn552 resolvase, the most closely related of known resolvases to the homologous DNA invertases, initially was identified as a DNA invertase ('Bin') as a result of its ability to mediate efficient inversion of this segment in vivo. PMID:2555186

Rowland, S J; Dyke, K G

1989-01-01

56

14. Roaring Fork Motor Nature Trail, Place of a thousand ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

57

Superfund Record of Decision (EPA Region 4): Milan Army Ammunition Plant, Milan, TN, March 11, 1998  

SciTech Connect

This decision document presents the selected action for the Salvage Yard, Former Ammunition Burnout Area (ABA), and Sanitary Landfill at MLAAP, located in Gibson and Carroll Counties, TN. This ROD addresses the final response action planned for the Salvage Yard, Former ABA, and Sanitary Landfill, including soil and groundwater. NFA is the selected remedy for soil and groundwater at the Salvage Yard, Former ABA, and Sanitary landfill. The selected remedy manages the risk to acceptable levels for both human health and the environment and is the final action planned.

NONE

1998-09-01

58

Structural and microstructural evolution of the Rattlesnake Mountain Anticline (Wyoming, USA): New insights into the Sevier and Laramide orogenic stress build-up in the Bighorn Basin  

NASA Astrophysics Data System (ADS)

The Rocky Mountains in western US provide among the best examples of thick-skinned tectonics: following a period of thin-skinned tectonics related to the Sevier orogeny, the compressional reactivation of basement faults gave birth to the so-called Laramide uplifts/arches. The Bighorn basin, located in Wyoming, is therefore a key place to study the transition from thin- to thick-skinned tectonics in orogenic forelands, especially in terms of microstructural and stress/strain evolution. Our study focuses on a classic Laramide structure: the Rattlesnake Mountain Anticline (RMA, Wyoming, USA), a basement-cored anticline located in the western part of the Bighorn basin. Stress and strain evolution analysis in folded sedimentary layers and underlying faulted basement rocks were performed on the basis of combined analyses of fractures, fault-slip data and calcite twinning paleopiezometry. Most of the fractures are related to three main tectonic events: the Sevier thin-skinned contraction, the Laramide thick-skinned contraction, and the Basin and Range extension. Serial balanced cross-sections of RMA and displacement profiles suggest that all thrust faults were coeval, evidencing strain distribution in the basement during faulting. The comparison of RMA with another structure located in the eastern edge of the Bighorn basin, i.e. the Sheep Mountain Anticline (SMA), allows to propose a conceptual model for the geometric and kinematic evolution of Laramide-related basement-cored anticlines. Finally, the stress evolution is reconstructed at both the fold scale and the basin scale. We show that the evolution of stress trends and magnitudes was quite similar in both structures (RMA and SMA) during Laramide times (thick-skinned tectonics), in spite of different stress regimes. During Sevier (thin-skinned tectonics) and post-Laramide times, stress trends and fracture patterns were different in these two structures. These results suggest that the distance to the orogenic front influenced the fracture patterns but not the foreland stress magnitudes, which were likely controlled by the structural style.

Beaudoin, Nicolas; Leprêtre, Rémi; Bellahsen, Nicolas; Lacombe, Olivier; Amrouch, Khalid; Callot, Jean-Paul; Emmanuel, Laurent; Daniel, Jean-Marc

2012-11-01

59

Geologic Map of the Pahranagat Range 30' x 60' Quadrangle, Lincoln and Nye Counties, Nevada  

USGS Publications Warehouse

Introduction The Pahranagat Range 30' x 60' quadrangle lies within an arid, sparsely populated part of Lincoln and Nye Counties, southeastern Nevada. Much of the area is public land that includes the Desert National Wildlife Range, the Pahranagat National Wildlife Refuge, and the Nellis Air Force Base. The topography, typical of much of the Basin and Range Province, consists of north-south-trending ranges and intervening broad alluvial valleys. Elevations range from about 1,000 to 2,900 m. At the regional scale, the Pahranagat Range quadrangle lies within the Mesozoic and early Tertiary Sevier Fold-and-Thrust Belt and the Cenozoic Basin and Range Province. The quadrangle is underlain by a Proterozoic to Permian miogeoclinal section, a nonmarine clastic and volcanic section of middle Oligocene or older to late Miocene age, and alluvial deposits of late Cenozoic age. The structural features that are exposed reflect relatively shallow crustal deformation. Mesozoic deformation is dominated by thrust faults and asymmetric or open folds. Cenozoic deformation is dominated by faults that dip more than 45i and dominostyle tilted blocks. At least three major tectonic events have affected the area: Mesozoic (Sevier) folding and thrust faulting, pre-middle Oligocene extensional deformation, and late Cenozoic (mainly late Miocene to Holocene) extensional deformation. Continued tectonic activity is expressed in the Pahranagat Range area by seismicity and faults having scarps that cut alluvial deposits.

Jayko, A.S.

2007-01-01

60

Fraction A of armadillo submandibular glycoprotein and its desialylated product as sialyl-Tn and Tn receptors for lectins.  

PubMed

Fraction A of the armadillo submandibular glycoprotein (ASG-A) is one of the simplest glycoproteins among mammalian salivary mucins. The carbohydrate side chains of this mucous glycoprotein have one-third of the NeuAc alpha 2-->6GalNAc (sialyl-Tn) sequence and two thirds of Tn (GalNAc alpha-->Ser/Thr) residues. Those of the desialylated product (ASG-Tn) are almost exclusively unsubstituted GalNAc residues (Tn determinant). When the binding properties of these glycoproteins were tested by a precipitin assay with Gal, GalNAc and GlcNAc specific lectins, it was found that ASG-Tn reacted strongly with all of the Tn-active lectins and completely precipitated Vicia villosa (VVL both B4 and mixture of A and B), Maclura pomifera (MPA), and Artocarpus integrifolia (jacalin) lectins. However, it precipitated poorly or negligibly with Ricinus communis (RCA1); Dolichos biflorus (DBA); Viscum album, ML-I; Arachis hypogaea (PNA), and Triticum vulgaris (WGA). The reactivity of ASG-A (sialyl-Tn) was as active as that of ASG-Tn with MPA and less or slightly less active than that of ASG-Tn with VVL-A+B, VVL-B4, HPA, WFA, and jacalin, as one-third of its Tn was sialylated. These findings indicate that ASG-A and its desialylated product (ASG-Tn) are highly useful reagents for the differentiation of Tn, T (Gal beta 1-->3GalNAc), A (GalNAc alpha 1-->3Gal) or Gal specific lectins and monoclonal antibodies against such epitopes. PMID:7875333

Wu, A M; Shen, F; Herp, A; Song, S C; Wu, J H

1995-02-27

61

78 FR 38555 - Amendment of Class E Airspace; Selmer, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...Instrument Flight Rules (IFR) operations at Robert Sibley Airport. This enhances the safety...NPRM) to amend Class E airspace at Robert Sibley Airport, Selmer, TN. (78 FR...surface within a 6.7-mile radius of Robert Sibley Airport, Selmer, TN....

2013-06-27

62

POSITION INFORMATION Position # X1225 Lewis County Program Area  

E-print Network

. · Develop and maintain expertise about issues important to agriculture and natural resources in the county: Headquarters: Extension Agent Agriculture Adult and Youth Hohenwald, TN Screening of applicants' credentials . BENEFITS Group hospitalization and life insurance; State Retirement; Workers' Compensation; study, sick

Grissino-Mayer, Henri D.

63

P-156 / H. Y. Mak P-156: Transflective Double Cell TN Liquid Crystal Display  

E-print Network

TFT-LCD. Keywords: transflective LCD; double cell TN-LCD, DTN. 1. INTRODUCTION Transflective LCDs. A double cell TN-LCD (DTN-LCD) consists of two TN liquid crystal layers. The twisted angle of the TN liquidP-156 / H. Y. Mak P-156: Transflective Double Cell TN Liquid Crystal Display H. Y. Mak and V

64

Magnitude, Timing, and Geometry of Extension in the Southern Sevier Desert Basin From Piercing Points, Seismic-Stratigraphic Reconstruction, and Deep well Data  

NASA Astrophysics Data System (ADS)

Palinspastic reconstruction of Mesozoic thrust sheets provides the main constraint for an estimated 47 km of Cenozoic extensional displacement along the Sevier Desert detachment (SDD) in the central Sevier Desert Basin. Hanging wall and footwall piercing points indicate that the SDD accommodated a minimum of 35 km of extensional displacement in the narrower southern part of the basin. The piercing points for the SDD are defined by the intersection of the SDD, the Canyon Range thrust (CRT), and a regional early Cenozoic erosion surface (ES). The hanging wall piercing point lies immediately northeast of the Cricket Mountains, where the SDD-CRT- ES intersection is narrowly defined by intersecting structure maps derived from published seismic reflection data. The footwall piercing point lies in the southern foothills of the Canyon Range, where the SDD breakaway plane is well constrained by an industry seismic line that lies within 2 km of the exposed intersection of the CRT with the base of the Oligocene Oak City Formation. Timing of extension in the southern Sevier Desert basin is constrained by a kinematic reconstruction of detachment and imbricate fault displacement, footwall uplift, and supradetachment sedimentation for Oligocene, Miocene, and Plio-Pleistocene seismic sequences. The reconstruction is centered on a seismic reflection and gravity interpretation along the published Pan Canadian profiles 2 and 3 that is tied to dated intervals in six industry wells. Fault restoration indicates that Oligocene and Miocene phases of slip each accounted for about 40 percent of the total displacement. Simultaneous backstripping of the Oligocene, Miocene, and Plio-Pleistocene supradetachment sequences records hanging wall subsidence simultaneous with footwall uplift, with a footwall burial history that is consistent with published Miocene apatite and zircon fission-track ages of footwall samples. The geometric evolution of the southern SDD extensional system is consistent with its development above a broad westward-migrating "rolling hinge" zone associated with isostatic uplift of the detachment footwall. Hanging wall normal faults east of the footwall crest exhibit small post-Miocene displacement, with demonstrable Quaternary slip restricted to the crest and western limb of the uplift, most notably along the Black Rock and Clear Lake fault zones. Early abandonment of the eastern part of the detachment may explain the indistinct geomorphic and structural expression of the break-away zone at the surface. The deepest level of the southern SDD also presents a complex geometry and kinematic history. The 1996 Chevron 1-29 Black Rock Federal well through the western basin margin penetrated a normal fault that places Jurassic over lower Cambrian strata at 4650 m measured depth, well above the principal SDD seismic reflection. The fault is not correlated to any large- displacement high-angle fault at shallow levels, and may form the abandoned roof to an extensional duplex.

Coogan, J. C.; Decelles, P. G.

2007-12-01

65

From Source to Sink: Exhumation of the North America Cordillera Revealed by Multi-dating of Detrital Minerals from Upper Jurassic-Upper Cretaceous Sevier Foreland Basin  

NASA Astrophysics Data System (ADS)

We sampled twenty-two Late Jurassic to Late Cretaceous syn-orogenic conglomerate clasts in proximal units in the Sevier fold-thrust belt and their distal sandstone equivalents up to 300 km east of the thrust front, in Utah, Colorado, Wyoming, and South Dakota. To better constrain depositional ages, these samples were analyzed using detrital zircon U-Pb (DZ U-Pb) geochronology. To identify a thermochronometer that measures source exhumation in the North America Cordillera, both zircon (U-Th)/He and apatite fission track (AFT) thermochronology was utilized, on both the conglomerate cobbles and sandstone (detrital) samples. Eleven samples were analyzed with zircon (U-Th)/He; however, discordant ages in the conglomerate cobble samples suggest that this system was not fully reset and never experienced T> ~180 °C in the source stratigraphy during the Sevier orogeny. Eleven other samples are analyzed using apatite fission track thermochronology (AFT); AFT ages are generally similar or older than depositional ages indicating that the detrital ages record source exhumation signals, and that exhumation depth corresponds to T>~120 °C. In order to test whether or not the youngest cooling AFT age population represents a source exhumation signal or a co-magmatic signal we here performed double dating of the detrital AFT samples using apatite U-Pb thermochronology. Maximum depositional ages using DZ U-Pb match existing age controls on basin stratigraphy. Our study shows that AFT is an effective thermochronometer to detect source exhumation for Cretaceous foreland stratigraphy in the western U.S.A. Lag-times (i.e. the difference between the source exhumation age and depositional age) are ~0 to 5 Myr with relatively steady-state to slightly increasing exhumation rates suggesting orogenic growth at this time. The very short lag times also indicate limited to no storage time between source and sink. The AFT lag time of the Early Cretaceous Kelvin Formation is ~5 Myr and represents a time when the Canyon Range Thrust was active to the southwest. Upper Cretaceous units have an approximate lag time of 0 Myr, during a time when the Willard, Crawford, Medicine Butte and Absaroka Thrust systems were active to the west. These lag-time measurements correspond to exhumation rates of ~0.8 mm/yr to >>1 mm/yr.

Painter, C. S.; Carrapa, B.; DeCelles, P. G.; Gehrels, G. E.; Thomson, S. N.

2013-12-01

66

Tn7 elements: Engendering diversity from chromosomes to episomes  

PubMed Central

The bacterial transposon Tn7 maintains two distinct lifestyles, one in horizontally transferred DNA and the other in bacterial chromosomes. Access to these two DNA pools is mediated by two separate target selection pathways. The proteins involved in these pathways have evolved to specifically activate transposition into their cognate target-sites using entirely different recognition mechanisms, but the same core transposition machinery. In this review we discuss how the molecular mechanisms of Tn7-like elements contribute to their diversification and how they affect the evolution of their host genomes. The analysis of over 50 Tn7-like elements provides insight into the evolution of Tn7 and Tn7 relatives. In addition to the genes required for transposition, Tn7-like elements transport a wide variety of genes that contribute to the success of diverse organisms. We propose that by decisively moving between mobile and stationary DNA pools, Tn7-like elements accumulate a broad range of genetic material, providing a selective advantage for diverse host bacteria. PMID:18951916

Parks, Adam R.; Peters, Joseph E.

2008-01-01

67

Sedimentology and provenance of Late Cretaceous(. )-Eocene Sheep Pass Formation conglomerates in hinterland of Sevier Orogenic Belt, east-central Nevada  

SciTech Connect

The Late Cretaceous(.)-Eocene Sheep Pass Formation of east-central Nevada is a thick (1500-m) sequence of nonmarine conglomerate, sandstone, mud rock, and limestone. Detailed stratigraphic analyses of the basal conglomerate (50 to 450 m thick) in the Egan, Pancake, and Fish Creek ranges was performed. The presence of large (up to 400 m) Paleozoic limestone slide blocks and paucity of sandstone lithofacies within conglomeratic sequences in the Egan Range suggest deposition on the proximal to medial portion of streamflow-dominated alluvial fans. Clast imbrication measurements document east-northeast to west-southwest paleoflow directions throughout the region. In the Egan and Pancake Ranges, gravel is comprised predominantly of Pennsylvanian-Permian carbonate and Mississippian quartzarenite (Scotty Wash). Gravels in the Fish Creek Range consist of paleozoic dolomite and Ordovician quartzarenite (Eureka) at the base of the Sheep Pass, and Paleozoic limestone and dolomite, Mississippian quartzarenite, and Ordovician quartzarenite near the top. Preliminary interpretations of our data indicate that Late Cretaceous-early Tertiary sedimentation in the hinterland of the Sevier orogenic belt occurred in a series of small basins characterized by general westward sediment dispersal patterns. Further interpretations of hinterland basin development and structural setting of basin-bounding uplifts await further work on Cretaceous-Eocene strata now in progress.

Diem, D.A.; Schmitt, J.G.

1986-08-01

68

76 FR 33656 - Television Broadcasting Services; Nashville, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...Part 73 [MB Docket No. 11-29; RM-11622, DA 11-949] Television Broadcasting Services; Nashville, TN AGENCY: Federal Communications...801(a)(1)(A). List of Subjects in 47 CFR Part 73 Television. Federal Communications Commission. Barbara A....

2011-06-09

69

78 FR 52424 - Amendment of Class E Airspace; Dayton, TN, Establishment of Class E Airspace; Cleveland, TN, and...  

Federal Register 2010, 2011, 2012, 2013, 2014

...establishes Class E Airspace at Cleveland Regional Jetport, Cleveland, TN, to accommodate area navigation (RNAV) global positioning system (GPS) SIAPs at the airport. Information regarding Bradley Memorial Hospital is added to the Cleveland,...

2013-08-23

70

Vertical transmission of TnSNPV, TnCPV, AcMNPV, and possibly recombinant NPV in Trichoplusia ni  

E-print Network

(TnCPV), Autographa californica nucleopolyhedrovirus (AcMNPV), and AcMNPV engineered to express). All rights reserved. Keywords: Trichoplusia ni; Nucleopolyhedrovirus; Trichoplusia ni NPV; Autographa californica NPV; Recombinant NPV; Cypovirus; Trichoplusia ni CPV; Transmission, viral; Transmission, vertical

Hammock, Bruce D.

71

Transposon mutagenesis in Desulfovibrio desulfuricans: development of a random mutagenesis tool from Tn7.  

PubMed Central

The transposons Tn5, Tn7, Tn9, and Tn10 or their derivatives have been examined for transposition in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20. Tn7 inserted with a frequency of 10(-4) to 10(-3) into a unique attachment site that shows strong homology with those sites identified in other gram-negative bacteria. Inactivation of the tnsD gene in Tn7, encoding the function directing insertion into the unique site, yielded a derivative that transposed essentially randomly with a frequency of ca. 10(-6) per donor. Derivatives of Tn5, but not wild-type Tn5, were also found to undergo random transposition at a similar frequency. No evidence was obtained for transposition of Tn9 or Tn10. PMID:8837431

Wall, J D; Murnan, T; Argyle, J; English, R S; Rapp-Giles, B J

1996-01-01

72

Laser-probe 40Ar/39Ar dating of strain fringes: Mid-Cretaceous synconvergent orogen-parallel extension in the interior of the Sevier orogen  

NASA Astrophysics Data System (ADS)

UV and CO2 laser-probe 40Ar/39Ar in situ analyses of phlogopite and muscovite in fibrous strain fringes from greenschist-facies metamorphic rocks document mica growth ages at temperatures lower than their closure temperatures, and therefore directly date deformation. The new dates resolve the age of the earliest ductile fabric recorded in the Raft River-Albion-Grouse Creek metamorphic core complex of Utah and Idaho. Phlogopite was dated in quartz-calcite-phlogopite strain fringes around pyrite in Pennsylvanian-Permian rocks from the Grouse Creek Mountains (Utah) using both the UV and CO2 laser probe; muscovite was dated in quartz-muscovite strain fringes around pyrite in deformed Jurassic sills from the Black Pine Mountains (Idaho) using the CO2 laser probe. Phlogopite 40Ar/39Ar ages for individual strain fringes (Grouse Creek Mountains) range from 92 Ma to 110 Ma, with the most reliable ages ranging from 101 Ma to 110 Ma (mean age, 105.0 ± 5.8 Ma). Muscovite 40Ar/39Ar ages for individual strain fringes (Black Pine Mountains) range from 97 Ma to 112 Ma (mean age, 104.7 ± 5.8 Ma). Strain fringes are associated with a subhorizontal foliation and a generally N-trending elongation lineation exhibiting components of top-to-the-north simple shear and coaxial strain accommodating N-S extension and subvertical shortening. Midcrustal northward flow at 105 (±6) Ma within the interior of the Sevier orogen, coeval with east-directed shortening in the foreland and with plate convergence, records orogen-parallel synconvergent extension. We favor gravitational relaxation of structural culminations resulting from focused crustal shortening as a driving mechanism for orogen-parallel flow.

Wells, Michael L.; Spell, Terry L.; Hoisch, Thomas D.; Arriola, Tonia; Zanetti, Kathleen A.

2008-06-01

73

8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY VIEW OF ABUTMENT SUPPORT INTO THE GROUND - Lost Creek Road Bridge, Spanning Sevier River at County Road 243, 2 miles east of Aurora, Aurora, Sevier County, UT

74

75 FR 6257 - Watts Bar Reservoir Land Management Plan, Loudon, Meigs, Rhea, and Roane Counties, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...TENNESSEE VALLEY AUTHORITY Watts Bar Reservoir Land Management Plan, Loudon, Meigs...acres of TVA public land on Watts Bar Reservoir in Tennessee. On November 19, 2009...statement (FEIS) for the Watts Bar Reservoir Land Management Plan (WBRLMP)....

2010-02-08

75

76 FR 5194 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN; Final...  

Federal Register 2010, 2011, 2012, 2013, 2014

...FWS-R4-R-2010-N172; 40136-1265-0000-S3] Tennessee National Wildlife Refuge, Henry, Benton...for the environmental assessment for Tennessee National Wildlife Refuge (NWR). In...by writing to: Mr. Troy Littrell, Tennessee National Wildlife Refuge, 3006...

2011-01-28

76

75 FR 32201 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...FWS-R4-R-2010-N050; 40136-1265-0000-S3] Tennessee National Wildlife Refuge, Henry, Benton...environmental assessment (Draft CCP/EA) for Tennessee National Wildlife Refuge (NWR) for...notice, we continue the CCP process for Tennessee NWR. We started the process...

2010-06-07

77

Powers in arithmetic progress (III) T.N. Shorey  

E-print Network

Powers in arithmetic progress (III) T.N. Shorey in arithmetic progression This is in contiuation of [30] and [31]. For an integer > 1, we denote result is due to Euler [see [18]) that a product of four terms in arithmetic progression

Shorey, T.N.

78

A comparison of Anisotropy of Magnetic Susceptibility Studies to Clast Based Strain Analysis in Sandstones, From The Outer Margin Of The Sevier Orogenic Foreland, Western Wyoming.  

NASA Astrophysics Data System (ADS)

The Cordilleran Mountain Belt of North America is one of the world's classic foreland fold and thrust belts. The Sevier Belt represents the thin skinned front of this orogeny, consisting of thrust faults and folds that shortened and transported sequences of Devonian to Cretaceous strata eastward. There is a general increase in deformation westwards which provides an ideal geological setting to explore the potential link between Anisotropy of Magnetic Susceptibility (AMS) and results from clast based strain analyses of sandstones. Studies attempting to define the relationship between AMS and finite strain have been in vogue since the link between layer parallel shortening and AMS was first established. The understanding of this relationship, despite proven strong correlations between the AMS tensors and tectonic directions, is complicated by competing sub-fabrics, as well as the various magnetic properties of the minerals contributing to the AMS fabric. It has become very clear that the sensitivity of AMS is capable of detecting incipient tectonic fabrics, <5% shortening, which is typically outside the range of most dedicated strain analyses. Despite this, there has been little published research into the relationship between the classic fabric analysis techniques or strain estimate methods and AMS. This may be due to the particularly laborious task of calculating strain estimates using large marker populations and/or the pitfalls of trying to relate the magnitudes of the magnetic ellipse to the magnitudes of the strain ellipse. Rather than trying to estimate finite strain directly from the AMS ellipsoid, we are using the ability of AMS to accurately and quickly qualify the petrofabric and determine the origin of that fabric (i.e., whether it is purely sedimentary, composite bedding/tectonic or dominantly tectonic etc.). Where as most methods of estimating strain have poor accuracy constraints in low strain regimes. In an attempt to account for this AMS is being utilised as a control for the sensitivity limits for these strain analysis methods, this is in part due to low sample counts, subjectivity and human error. This research uses recently developed and remodified strain analysis methods that are being utilised in a Mathematica environment. The use of Mathematica allows for objective, swift, reproducible and automated calculation of a suite of strain estimate techniques, such as the Fry method, a variation of Ramsay's Nearest Neighbour Method and the Mean Radial Length Method.

Mc Carthy, Dave; Meere, Pat; Petronis, Mike; Mulchrone, Kieran

2013-04-01

79

The Lower Sevier River Basin Crop Monitor and Forecast Decision Support System: Exploiting Landsat Imagery to Provide Continuous Information to Farmers and Water Managers  

NASA Astrophysics Data System (ADS)

The last century has seen a large number of innovations in agriculture such as better policies for water control and management, upgraded water conveyance, irrigation, distribution, and monitoring systems, and better weather forecasting products. In spite of this, irrigation management and irrigation water deliveries by farmers/water managers is still based on factors like water share amounts, tradition, and past experience on irrigation. These factors are not necessarily related to the actual crop water use; they are followed because of the absence of related information provided in a timely manner at an affordable cost. Thus, it is necessary to develop means to deliver continuous and personalized information about crop water requirements to water users/managers at the field and irrigation system levels so managers at these levels can better quantify the required versus available water for irrigation during the irrigation season. This study presents a new decision support system (DSS) platform that addresses the absence of information on actual crop water requirements and crop performance by providing continuous updated farm-based crop water use along with other farm performance indicators such as crop yield and farm management to irrigators and water managers. This DSS exploits the periodicity of the Landsat Satellite Mission (8 to 16 days, depending on the period of interest) to provide remote monitoring at the individual field and irrigation system levels. The Landsat satellite images are converted into information about crop water use, yield performance and field management through application of state-of-the-art semi-physical and statistical algorithms that provide this information at a pixel basis that are ultimately aggregated to field and irrigation system levels. A version of the DSS has been implemented for the agricultural lands in the Lower Sevier River, Utah, and has been operational since the beginning of the 2013 irrigation season. The main goal of this DSS implementation is to provide continuous and personalized information to farmers and water managers regarding crops in fields and the irrigation delivery system throughout the irrigation season so that decisions related to agricultural water use can result in water savings while not diminishing crop yields.

Torres-Rua, A. F.; Walker, W. R.; McKee, M.

2013-12-01

80

Month Dates Days Activity Location Deadline Cost Sept. 7 Sat. Hiking Great Stone Door, SCRA, TN 8/30 $26  

E-print Network

Month Dates Days Activity Location Deadline Cost Sept. 7 Sat. Hiking Great Stone Door, SCRA, TN 8! Oct. 5 Sat. Bouldering Stone Fort, TN 9/27 $41 5-6 Sat. - Sun. Camping and Hiking Bells Bend Park, TN Clean-up / Canoeing Buffalo River, TN 10/4* Free! 20 Sun. Hiking Walls of Jericho, TN 10/4* $24 26 Sat

Bordenstein, Seth

81

Interaction of hamster submaxillary sialyl-Tn and Tn glycoproteins with Gal, GalNAc and GlcNAc specific lectins.  

PubMed

Hamster submaxillary glycoprotein (HSM), one of the simplest glycoproteins among mammalian salivary mucins, is composed of approximately equivalent amounts of protein, hexosamine and sialic acid. The Thr and Ser residues in the protein core account for more than half of all of the amino acid residues, while Lys, Glu, Pro and Ala are the major components of the remaining portion of amino acids. The carbohydrate side chains of this mucous glycoprotein have mainly the NeuAc-GalNAc-(sialyl-Tn) sequence (HSM), and those of the desialylated product (HSM-Tn) are almost exclusively unsubstituted GalNAc residues (Tn determinants). The binding properties of sialyl-Tn (HSM) and asialo-HSM (HSM-Tn) glycoproteins were tested by precipitin assay with Gal, GalNAc and GlcNAc specific lectins. The HSM-Tn completely precipitated Vicia villosa (VVL both B4 and mixture of A and B), Maclura pomifera (MPL), and Artocarpus integrifolia (Jacalin) lectins; less than 2 micrograms of HSM-Tn were required for precipitating 50% of 5.0-6.3 micrograms lectin nitrogen added. HSM-Tn also reacted well with Helix pomatia lectin (HPL), Wistaria floribunda lectin (WFL) and Abrus precatorius agglutinin (APA) and precipitated in each case over 81% of the lectin nitrogen added. The reactivity of HSM-Tn with other lectins (Ricinus communis, RCA1; Dolichol biflorus, DBL; Viscum album, ML-I; Arachis hypogaea, PNA, and Triticum vulgaris, WGA) was weak or negligible. The activity of sialyl-Tn (HSM) was more restricted; HSM reacted well with Jacalin, moderately with MPL and VVL-B4, but was inactive or only weakly with the other lectins used. These findings indicate that HSM and its desialylated product (HSM-Tn) are highly useful reagents for the differentiation of Tn and T/Gal specific lectins and for anti-T, Tn and Af monoclonal antibodies. PMID:8183285

Wu, A M; Shen, F; Herp, A; Wu, J H

1994-04-01

82

76 FR 58726 - Proposed Amendment of Class E Airspace; Springfield, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...in the Springfield, TN area. Aydelotte Airport has been abandoned and controlled airspace is no longer needed. Airspace reconfiguration...in the Springfield, TN area. Aydelotte Airport has been abandoned making it necessary to remove controlled airspace...

2011-09-22

83

Site-specific properties of Tn 7 transposition into the E. coli Chromosome  

Microsoft Academic Search

A study has been made of the insertional properties of transposon Tn7, a 14 kilobase transposable element encoding resistances to trimethoprim, streptomycin and specitinomycin. It has previously been shown that Tn7 transposes at a low frequency and with low specificity into multiple sites in large transmissible plasmids. However, Tn7 transposes with extrame specificity and at high efficiency into the E.

Conrad Lichtenstein; Sydney Brenner

1981-01-01

84

Tumor-associated Neu5Ac-Tn and Neu5Gc-Tn antigens bind to C-type lectin CLEC10A (CD301, MGL).  

PubMed

In human tumors, glycoproteins often exhibit abnormal glycosylation patterns, e.g. certain Lewis structures, TF antigen, Tn antigen and/or their sialylated forms, creating additional binding sites for glycoreceptors. In the present study, we have analyzed the carbohydrate specificity of the C-type lectin CLEC10A using glycan profiling by enzyme-linked immunosorbent assay (ELISA). In addition to the known ligands, we show binding to two tumor-associated antigens, namely Neu5Ac?2,6-Tn and Neu5Gc?2,6-Tn, with an affinity of CLEC10A in the micromolar range. Detailed analyses of the glycan-lectin interactions were carried out by surface plasmon resonance (SPR) and saturation transfer difference (STD) NMR. CLEC10A binds Neu5Ac?2,6-Tn and Neu5Gc?2,6-Tn with dissociation constants of 297 and 80 µM, respectively, as determined by SPR. Comparison of the STD nuclear magnetic resonance (NMR) binding epitopes of Tn and Neu5Ac?2,6-Tn revealed a constant binding mode of the N-acetylgalactosamine moiety. This finding is in good agreement with binding studies of CLEC10A transfectomas, which show a well-defined interaction of transmembrane CLEC10A with 6-sialylated-Tn structures. Since both Neu5Ac?2,6-Tn and Neu5Gc?2,6-Tn together with the previously known Tn antigen are expressed in human tumors such as mammary carcinoma, the interaction with CLEC10A expressed by macrophages and dendritic cells could be of major functional significance in tumor progression. PMID:23507963

Mortezai, Naghmeh; Behnken, Henning N; Kurze, Anna-Katharina; Ludewig, Peter; Buck, Friedrich; Meyer, Bernd; Wagener, Christoph

2013-07-01

85

78 FR 7993 - Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...No. 11-ASO-28] Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN AGENCY: Federal Aviation...SUMMARY: This action amends Class D and Class E airspace and removes Class E airspace in the...

2013-02-05

86

Balance : Lancaster County's tragedy  

E-print Network

Lancaster County, Pennsylvania residents are proud of their agricultural heritage. They do not want to see their farmland disappear. But the County continues to be developed into residential subdivisions. This thesis ...

Gingrich, Valerie (Valerie J.)

2007-01-01

87

Nucleotide Sequence Analysis of Integrative Conjugative Element Tn5253 of Streptococcus pneumoniae  

PubMed Central

Conjugative transposon Tn5253, an integrative conjugative element (ICE) of Streptococcus pneumoniae carrying the cat and tet(M) genes, was shown to be 64,528 bp in size and to contain 79 open reading frames, of which only 38 could be annotated. Two distinct genetic elements were found integrated into Tn5253: Tn5251 (18,033 bp), of the Tn916-Tn1545 family of ICEs, and ?cat(pC194) (7,627 bp), which could not conjugate but was capable of intracellular mobility by excision, circularization, and integration by homologous recombination. The highest conjugation frequency of Tn5253 was observed when Streptococcus pyogenes was the donor (6.7 × 10?3 transconjugants/donor). PMID:24295984

Iannelli, Francesco; Santoro, Francesco; Oggioni, Marco R.

2014-01-01

88

Percent Uninsured by County  

NSDL National Science Digital Library

This is a county by county visualization of the percentage of residents that are uninsured. The data are from a set available here: http://www-958.ibm.com/software/data/cognos/manyeyes/datasets/percent-uninsured-by-county/versions/1

Manyeyes

89

A restriction enzyme cleavage map of Tn 5 and location of a region encoding neomycin resistance  

Microsoft Academic Search

This paper reports a cleavage site map of Tn5 for restriction enzymes BamHI, BglI, BglII, HindII, HindIII, HpaI, SalI, AvaI, SmaI, XhoI, PstI, PvuII, HaeII and HaeIII that was determined by the analysis of restriction enzyme cleavage patterns of ColEl, two independent ColE1::Tn5 plasmids, and a ColE1::Tn5 deletion derivative. BalI, EcoRI, KpnI, and PvuI do not cleave Tn5. Construction and

Richard A. Jorgensen; Steven J. Rothstein; Williams S. Reznikoff

1979-01-01

90

Conduction of pEC22, a plasmid coding for MR.EcoT22I, mediated by a resident Tn3-like transposon, Tn5396.  

PubMed Central

pEC22 is a small plasmid that encodes the restriction-modification system MR.EcoT22I. Restriction and functional analysis of the plasmid identified the positions of genes encoding that system. The plasmid is able to be conducted by conjugal plasmids, a process mediated by a transposon contained within pEC22. This cryptic transposon, called Tn5396, was isolated from pEC22 and partially sequenced. The sequence of Tn5396 is for the most part typical of transposons of the Tn3 family and is most similar to that of Tn1000. The transposon differs from closely related transposons in that it lacks well-conserved sequences in the inverted-repeat region and has an unusually long terminal inverted repeat. Consideration of regions of internal sequence similarity in this and other transposons in the Tn3 family supports a theory of the mechanism by which the ends of Tn3-like transposons may maintain substantial identity between their inverted repeats over the course of evolutionary time. PMID:8051018

Elhai, J; Cai, Y; Wolk, C P

1994-01-01

91

78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...the Tri-Cities, TN area, by correcting the regulatory text of the Class E surface airspace at Tri-Cities...Heliport in the Class E surface area airspace description for...Airspace Designated as Surface Areas * * * * * ASO TN E2...

2013-07-23

92

Molecular characterization of the class II multiresistance transposable element Tn1403 from Pseudomonas aeruginosa.  

PubMed Central

Transposon Tn1403 is a 19.9-kb multiresistance class II transposable element originally found on the RPL11 plasmid from a clinical isolate of Pseudomonas aeruginosa. It encodes resistance to ampicillin (PSE-1 beta-lactamase), streptomycin and spectinomycin (aadA and aphC), and chloramphenicol (cat). It has structural homology with the tnpM and tnpI sequences of Tn21 and inverted repeats and res and tnpR sequences of Tn501, but it has no structural homology nor functional complementation with the resolvase gene of Tn21 or Tn3. Sequence analysis revealed long inverted repeats at each extremity of Tn1403 containing 38-bp inverted repeats that were 97.4% similar to those of Tn1721 and 5-bp direct repeats. Transposition assays showed a low frequency of transposition (3.5 x 10(-6)) compared with that of Tn3 (3.3 x 10(-3)) and no resolution of cointegrates. Images PMID:1850969

Vézina, G; Levesque, R C

1991-01-01

93

Introduction of transposon Tn5 into Myxococcus for analysis of developmental and other nonselectable mutants  

PubMed Central

The transposon Tn5, which carries a gene for kanamycin resistance, can be introduced into Myxococcus xanthus, an organism that undergoes a primitive cycle of development, from Escherichia coli by the specialized transducing phage P1::Tn5. Tn5 DNA sequences, but no P1 sequences, are found in the stable kanamycin-resistant transductants. Tn5 transposes from P1 to many different chromosomal sites in Myxococcus. In each independent transductant of Myxococcus examined, the Tn5 element is found in a different DNA fragment produced by cleaving cell DNA with a restriction endonuclease. Moreover, different Tn5 insertions have been found linked to the first 20 different genetic sites tested. Once inserted into the Myxococcus chromosome, Tn5 remains fixed in position during growth and when transferred to another strain by generalized transduction. To analyze developmental or other mutants that have no selectable phenotype themselves, a general method has been devised, and tested, for the systematic isolation of a Tn5 insertion near any arbitrary locus. Images PMID:16592958

Kuner, Jerry M.; Kaiser, Dale

1981-01-01

94

ERDC TN-DOER-D4 Particle Tracking Model (PTM) in the SMS  

E-print Network

ERDC TN-DOER-D4 July 2005 Particle Tracking Model (PTM) in the SMS: I. Graphical Interface PURPOSE. The SMS interface of the PTM includes commands for layout of the model inputs including sediment sources simulation in SMS. Result files for these fields are then used as input to a PTM simulation. #12;ERDC TN

US Army Corps of Engineers

95

Geological and petrophysical characterization of the Ferron Sandstone for 3-D simulation of a fluvial-deltaic reservoir. Deliverable 1.4.4: Ferron Sandstone lithofacies and case-study areas, Emery and Sevier Counties, Utah  

SciTech Connect

The types of dominantly sandstone lithofacies that characterize the Cretaceous Ferron Sandstone reservoir types were tentatively identified before the project began. These reservoir types were defined and mapped at the regional scale and are the subject of the detailed, highly focused case studies. The purpose of conducting detailed case-study analysis is to develop a comprehensive, interdisciplinary, and quantitative characterization of fluvial-deltaic reservoirs which will allow realistic inter-well and reservoir-scale modeling to be used for improved oil-field development in actual reservoirs world-wide. The resulting benefits and value may: (1) increase recoverable reserves by identifying untapped compartments created by reservoir heterogeneity, (2) reduce development costs by more efficiently siting infill drilling locations, (3) increase deliverability by exploiting the reservoir along optimal fluid-flow paths, (4) enhance the application of new technologies, such as horizontal drilling, by identifying optimal drilling directions to maximize fluid-flow, and (5) identify reservoir trends for field extension drilling. Various geologic studies of the Ferron Sandstone were reviewed to compile a list of locations and types of lithofacies in the Ferron Sandstone to be examined in greater detail as part of the subsequent case studies. Preliminary regional interpretations were also used to help select the type and location of lithofacies for case studies. Potential case-study sites were delineated during several reconnaissance field trips by the geologic team. Two case-study sites were selected for the project: Ivie Creek and Willow Springs Wash, in the central and southern parts respectively of the project study area. Results are discussed.

Allison, M.L.

1996-01-04

96

Tn6188 - A Novel Transposon in Listeria monocytogenes Responsible for Tolerance to Benzalkonium Chloride  

PubMed Central

Controlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S.) aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC) as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR) transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs) (28.5 ± 4.7 mg/l) than strains without Tn6188 (14 ± 3.2 mg/l). Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that ?qacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L. monocytogenes strains. PMID:24098567

Muhterem-Uyar, Meryem; Zaiser, Andreas; Stessl, Beatrix; Guinane, Caitriona M.; Cotter, Paul D.; Wagner, Martin; Schmitz-Esser, Stephan

2013-01-01

97

International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21?  

PubMed Central

TEM-24 remains one of the most widespread TEM-type extended-spectrum ?-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-?Tn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly penetrating clones and highly promiscuous plasmids in the spread of antibiotic resistance, and it contributes to the elucidation of the origin and evolution of TEM-2 ESBL derivatives. PMID:19995930

Novais, Ângela; Baquero, Fernando; Machado, Elisabete; Cantón, Rafael; Peixe, Luísa; Coque, Teresa M.

2010-01-01

98

Adult teleost heart expresses two distinct troponin C paralogs: cardiac TnC and a novel and teleost-specific ssTnC in a chamber- and temperature-dependent manner.  

PubMed

The teleost-specific whole genome duplication created multiple copies of genes allowing for subfunctionalization of isoforms. In this study, we show that the teleost cardiac Ca2+-binding troponin C (TnC) is the product of two distinct genes: cardiac TnC (cTnC, TnnC1a) and a fish-specific slow skeletal TnC (ssTnC, TnnC1b). The ssTnC gene is novel to teleosts as mammals have a single gene commonly referred as cTnC but which is also expressed in slow skeletal muscle. In teleosts, the data strongly indicate that these are two TnC genes are different paralogs. Because we determined that ssTnC exists across many teleosts but not in basal ray-finned fish (e.g., bichir), we propose that these paralogs are the result of an ancestral tandem gene duplication persisting only in teleosts. Quantification of mRNA levels was used to demonstrate distinct expression localization patterns of the paralogs within the chambers of the heart. In the adult zebrafish acclimated at 28°C, ssTnC mRNA levels are twofold greater than cTnC mRNA levels in the atrium, whereas cTnC mRNA was almost exclusively expressed in the ventricle. Meanwhile, rainbow trout acclimated at 5°C showed cTnC mRNA levels in both chambers significantly greater than ssTnC. Distinct responses to temperature acclimation were also quantified in both adult zebrafish and rainbow trout, with mRNA in both chambers shifting to express higher levels of cTnC in 18°C acclimated zebrafish and 5°C acclimated trout. Possible subfunctionalization of TnC isoforms may provide insight into how teleosts achieve physiological versatility in chamber-specific contractile properties. PMID:23881286

Genge, Christine E; Davidson, William S; Tibbits, Glen F

2013-09-16

99

Careers 2011 April 7, 2011 1. 21st Mortgage Corp 620 Market ST Knoxville TN 37902 Gene Crabtree  

E-print Network

Kelly Mullins 5. Bristol TN Police Dept 801 Anderson ST Bristol TN 37620 Grant Hale 6. Carson Newman;33. TruPoint Bank PO Box 1010 Grundy VA 24614 Garnette Owens 34. Tusculum College 420 W. Morris Blvd

Karsai, Istvan

100

Expression of the Transposase Gene tnpA of Tn4652 Is Positively Affected by Integration Host Factor  

Microsoft Academic Search

Tn4652 is a derivative of the toluene degradation transposon Tn4651 that belongs to the Tn3 family of transposons (M. Tsuda and T. Iino, Mol. Gen. Genet. 210:270-276, 1987). We have sequenced the transposase gene tnpA of transposon Tn4652 and mapped its promoter to the right end of the element. The deduced amino acid sequence of tnpA revealed 96.2% identity with

RITA HORAK; MAIA KIVISAAR

1998-01-01

101

Month Dates Days Activity Location Deadline Cost Feb. 8 Sat. Hiking Honey Creek SNA, TN 1/31 $29  

E-print Network

Month Dates Days Activity Location Deadline Cost Feb. 8 Sat. Hiking Honey Creek SNA, TN 1/31 $29 15. Whitewater Kayaking Clear Creek, TN 3/21 $75 30 Sun. Hiking Fiery Gizzard Trail, TN 3/21 $21 April 4-5 Fri, TN 4/4 Free! 13 Sun. Hiking Short Springs and Old Stone Fort 4/4 $19 19 Sat. Climbing - Beginner

Simaan, Nabil

102

Epigenetic Silencing of the Chaperone Cosmc in Human Leukocytes Expressing Tn Antigen*  

PubMed Central

Cosmc is the specific molecular chaperone in the endoplasmic reticulum for T-synthase, a Golgi ?3-galactosyltransferase that generates the core 1 O-glycan, Gal?1–3GalNAc?-Ser/Thr, in glycoproteins. Dysfunctional Cosmc results in the formation of inactive T-synthase and consequent expression of the Tn antigen (GalNAc?1-Ser/Thr), which is associated with several human diseases. However, the molecular regulation of expression of Cosmc, which is encoded by a single gene on Xq24, is poorly understood. Here we show that epigenetic silencing of Cosmc through hypermethylation of its promoter leads to loss of Cosmc transcripts in Tn4 cells, an immortalized B cell line from a male patient with a Tn-syndrome-like phenotype. These cells lack T-synthase activity and express the Tn antigen. Treatment of cells with 5-aza-2?-deoxycytidine causes restoration of Cosmc transcripts, restores T-synthase activity, and reduces Tn antigen expression. Bisulfite sequencing shows that CG dinucleotides in the Cosmc core promoter are hypermethylated. Interestingly, several other X-linked genes associated with glycosylation are not silenced in Tn4 cells, and we observed no correlation of a particular DNA methyltransferase to aberrant methylation of Cosmc in these cells. Thus, hypermethylation of the Cosmc promoter in Tn4 cells is relatively specific. Epigenetic silencing of Cosmc provides another mechanism underlying the abnormal expression of the Tn antigen, which may be important in understanding aberrant Tn antigen expression in human diseases, including IgA nephropathy and cancer. PMID:23035125

Mi, Rongjuan; Song, Lina; Wang, Yingchun; Ding, Xiaokun; Zeng, Junwei; Lehoux, Sylvain; Aryal, Rajindra P.; Wang, Jianmei; Crew, Vanja K.; van Die, Irma; Chapman, Arlene B.; Cummings, Richard D.; Ju, Tongzhong

2012-01-01

103

Brownfield to Brightfield Initiative in Oak Ridge, TN - 12346  

SciTech Connect

Experience characterizing, permitting, and restoring 'Brownfield' sites-government or industrial sites with restricted future use due to the presence or potential presence of hazardous substances, pollutants, or contaminants-is being leveraged to identify opportunities for redevelopment into solar power generating facilities which, in this context, are called 'Brightfields'. Brownfield sites offer the expansive land necessary for large photovoltaic (PV) solar farms, but require an in-depth working knowledge of complicated regulatory restrictions and environmental constraints to develop them. As a part of the effort to identify opportunities for redevelopment of Brownfield sites for solar applications, a technical guide, was composed specifically for the development of solar generation on restricted use sites. The basis of the technical guide gives specific consideration to environmental requirements and installation methods breaking that into three areas for assessing: 1) levels of contamination, 2) ground penetration requirements, and 3) the requirements for aesthetics and maintenance. Brightfield projects are underway to support the technical guide and expand re-industrialization efforts for the former DOE Gaseous Diffusion Plant in Oak Ridge, TN. There are exciting opportunities to turn Brownfields into Brightfield solar energy solutions for meeting the future renewable energy needs of our country. Brownfields that offer the large surface area required for solar PV farms coupled with the technical guide for the installation of solar farms on restricted use sites supports efforts to develop the solar capacities and expertise to tap this future market. The initial projects designed following the technical guide will provide verification of the installation requirements and beneficial reuse of restricted use sites. (authors)

Hough, Gil; Fairless, Chad [Restoration Services, Inc. (RSI), Oak Ridge, Tennessee (United States)

2012-07-01

104

Highly preferred site of insertion of Tn7 into the chromosome of Vibrio anguillarum.  

PubMed

The possible usefulness of Tn7 as a tool for genetic studies in Vibrio anguillarum was examined. Using the plasmid pRK2073 as the transposon donor, Tn7 transposes at high frequency into the chromosome of V. anguillarum. However, hybridization analysis of the mutants DNA digested with different enzymes revealed that all isolates have the insertions in the same site. This indicates that like in many other gram-negative bacteria, Tn7 shows a specificity of transposition in the chromosome of V. anguillarum. Plasmid pRK2013 proved to be a very useful delivery vector for transposon mutagenesis in V. anguillarum. PMID:1319594

Lemos, M L; Crosa, J H

1992-03-01

105

Transfer of Vancomycin Resistance Transposon Tn1549 from Clostridium symbiosum to Enterococcus spp. in the Gut of Gnotobiotic Mice  

Microsoft Academic Search

The vancomycin resistance vanB2 gene cluster is disseminated worldwide and has been found in phyloge- netically remote bacterial genera. The vanB2 operon is part of conjugative transposons Tn1549\\/Tn5382, but conjugative transposition of these elements has not been demonstrated. We have obtained transfer of a Tn1549-like element (referred to herein as \\

Aline Launay; Susan A. Ballard; Paul D. R. Johnson; M. Lindsay Grayson; Thierry Lambert

2006-01-01

106

77 FR 21505 - Proposed Amendment of Class D and E Airspace; Blountville, TN, and Revocation of Class E Airspace...  

Federal Register 2010, 2011, 2012, 2013, 2014

...11-ASO-28] Proposed Amendment of Class D and E Airspace; Blountville, TN, and Revocation of Class E Airspace; Tri-City, TN AGENCY: Federal Aviation...This action proposes to amend Class D and Class E surface airspace at Blountville, TN, and...

2012-04-10

107

Pontotoc County Government Summer  

E-print Network

Pontotoc County Government Summer Youth Internship Program June 17 - 21, 2013 Sponsored By Government Summer Youth Internship Program June 17-21, 2013 Who: Youth ages 14-19 who attend a Pontotoc 24, 2013. What: Learn About YOUR Pontotoc County Government. Youth will spend time in each

Balasundaram, Balabhaskar "Baski"

108

Allegheny County Economic Trends  

E-print Network

. In 2005, economic activity in Allegheny County is estimated to produce over $77 billion in value added product. This value added production, called Gross Regional Product, accounts for over 72% of whatAllegheny County Economic Trends Prepared by: University Center for Social and Urban Research

Sibille, Etienne

109

Continuing Education for County Officials The duties and responsibilities of county government  

E-print Network

Continuing Education for County Officials The duties and responsibilities of county government, the V.G. Young Institute of County Government provides continuing education programs for local government officials, including county judges and commissioners, county treasurers, county and district

110

Direct interaction between the TnsA and TnsB subunits controls the heteromeric Tn7 transposase  

PubMed Central

The transposon Tn7 transposase that recognizes the transposon ends and mediates breakage and joining is heteromeric. It contains the Tn7-encoded proteins TnsB, which binds specifically to the transposon ends and carries out breakage and joining at the 3? ends, and TnsA, which carries out breakage at the 5? ends of Tn7. TnsA apparently does not bind specifically to DNA, and we have hypothesized that it is recruited to the ends by interaction with TnsB. In this work, we show that TnsA and TnsB interact directly and identify several TnsA and TnsB amino acids involved in this interaction. We also show that TnsA can stimulate two key activities of TnsB, specific binding to the ends and pairing of the Tn7 ends. The ends of Tn7 are structurally asymmetric (i.e., contain different numbers of TnsB-binding sites), and Tn7 also is functionally asymmetric, inserting into its specific target site, attachment site attTn7 (attTn7) in a single orientation. Moreover, Tn7 elements containing two Tn7 right ends can transpose, but elements with two Tn7 left ends cannot. We show here that TnsA + TnsB are unable to pair the ends of a Tn7 element containing two Tn7 left ends. This pairing defect likely contributes to the inability of Tn7 elements with two Tn7 left ends to transpose. PMID:23674682

Choi, Ki Young; Li, Ying; Sarnovsky, Robert; Craig, Nancy L.

2013-01-01

111

Multicopy integration of mini-Tn7 transposons into selected chromosomal sites of a Salmonella vaccine strain.  

PubMed

Chromosomal integration of expression modules for transgenes is an important aspect for the development of novel Salmonella vectors. Mini-Tn7 transposons have been used for the insertion of one such module into the chromosomal site attTn7, present only once in most Gram-negative bacteria. However, integration of multiple mini-Tn7 copies might be suitable for expression of appropriate amounts of antigen or combination of different modules. Here we demonstrate that integration of a 9.6?kb mini-Tn7 harbouring the luciferase luxCDABE (lux) occurs at the natural attTn7 site and simultaneously other locations of the Salmonella chromosome, which were engineered using ?-Red recombinase to contain one or two additional artificial attTn7 sites (a-attTn7). Multicopy integration even at closely spaced attTn7 sites was unexpected in light of the previously reported distance-dependent Tn7 target immunity. Integration of multiple copies of a mini-Tn7 containing a gfp cassette resulted in increasing green fluorescence of bacteria. Stable consecutive integration of two mini-Tn7 encoding lacZ and lux was achieved by initial transposition of lacZ-mini-Tn7, subsequent chromosomal insertion of a-attTn7 and a second round of transposition with lux-mini-Tn7. Mini-Tn7 thus constitutes a versatile method for multicopy integration of expression cassettes into the chromosome of Salmonella and possibly other bacteria. PMID:25488129

Roos, Karen; Werner, Esther; Loessner, Holger

2014-12-01

112

78 FR 16202 - Proposed Amendment of Class E Airspace; Selmer, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...Approach Procedures have been developed at Robert Sibley Airport. This action would enhance...Instrument Approach Procedures developed at Robert Sibley Airport, Selmer, TN. The geographic coordinates of Robert Sibley Airport would be adjusted to...

2013-03-14

113

Tn4563 transposition in Streptomyces coelicolor and its application to isolation of new morphological mutants.  

PubMed Central

The Tn3-like transposon Tn4556 (and its derivatives Tn4560 and Tn4563) has been used for insertion mapping of genetic loci cloned on plasmids, but it has been difficult to obtain chromosomal insertions, largely because of the lack of a strong selection against transposon donor molecules. In this communication, we report two efficient selection techniques for transposition and their use in the isolation of chromosomal insertion mutations. A number of independent Streptomyces coelicolor morphological mutants (bld and whi) were obtained. Two of the bld mutations were mapped to locations on the chromosome by SCP1-mediated conjugation; at least one mutation, bld-5m1, appears to define a novel locus involved in control of S. coelicolor morphogenesis and antibiotic production. Images PMID:1650343

Schauer, A T; Nelson, A D; Daniel, J B

1991-01-01

114

76 FR 12306 - Revisions to the California State Implementation Plan, Imperial County, Kern County, and Ventura...  

Federal Register 2010, 2011, 2012, 2013, 2014

...County, and Ventura County; Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District...

2011-03-07

115

76 FR 12280 - Revisions to the California State Implementation Plan, for Imperial County, Kern County, and...  

Federal Register 2010, 2011, 2012, 2013, 2014

...County, and Ventura County; Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District...

2011-03-07

116

A Tn7-based broad-range bacterial cloning and expression system  

Microsoft Academic Search

For many bacteria, cloning and expression systems are either scarce or nonexistent. We constructed several mini-Tn7 vectors and evaluated their potential as broad-range cloning and expression systems. In bacteria with a single chromosome, including Pseudomonas aeruginosa, Pseudomonas putida and Yersinia pestis, and in the presence of a helper plasmid encoding the site-specific transposition pathway, site- and orientation-specific Tn7 insertions occurred

Kyoung-Hee Choi; Jared B Gaynor; Kimberly G White; Carolina Lopez; Catharine M Bosio; RoxAnn R Karkhoff-Schweizer; Herbert P Schweizer

2005-01-01

117

Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903.  

PubMed Central

The kanamycin resistance transposon Tn903 consists of a unique region of about 1000 base pairs bounded by a pair of 1050-base-pair inverted repeat sequences. Each repeat contains two Pvu II endonuclease cleavage sites separated by 520 base pairs. We have constructed derivatives of Tn903 in which this 520-base-pair fragment is deleted from one or both repeats. Those derivatives that lack both 520-base-pair fragments cannot transpose, whereas those that lack just one remain transposition proficient. One such transposable derivative, Tn903 delta I, has been selected for further study. We have determined the sequence of the intact inverted repeat. The 18 base pairs at each end are identical and inverted relative to one another, a structure characteristic of insertion sequences. Additional experiments indicate that a single inverted repeat from Tn903 can, in fact, transpose; we propose that this element be called IS903. To correlate the DNA sequence with genetic activities, we have created mutations by inserting a 10-base-pair DNA fragment at several sites within the intact repeat of Tn903 delta 1, and we have examined the effect of such insertions on transposability. The results suggest that IS903 encodes a 307-amino-acid polypeptide (a "transposase") that is absolutely required for transposition of IS903 or Tn903. Images PMID:6261245

Grindley, N D; Joyce, C M

1980-01-01

118

Wisconsin County Histories  

NSDL National Science Digital Library

Whether you're interested in Reedsburg, Rhinelander, or Rubicon, the Wisconsin County Histories website will not fail those keen on the history of the Badger State. Created by the Wisconsin Historical Society, this archive provides access to more than 80 standard histories of Wisconsin counties, most of which were published between 1850 and 1920. The majority of the volumes are over several hundred pages long, and they include detailed passages on cities within their respective counties, along with sketches of prominent leaders. Visitors can use the drop-down menu available on the homepage to find specific volumes, or they can also perform a full text search across all of the histories.

119

Molecular Effects of Familial Hypertrophic Cardiomyopathy-Related Mutations in the TNT1 Domain of cTnT  

PubMed Central

Familial hypertrophic cardiomyopathy (FHC) is one of the most common genetic causes of heart disease. Approximately 15% of FHC-related mutations are found in cTnT [cardiac troponin (cTn) T]. Most of the cTnT FHC-related mutations are in or flanking the N-tail TNT1 domain that directly interacts with overlapping tropomyosin (Tm). We investigate two sets of cTnT mutations at opposite ends of TNT1, mutations in residue 92 in the Tm–Tm overlap region of TNT1 and mutations in residues 160 and 163 in the C-terminal portion of TNT1 adjacent to the cTnT H1–H2 linker. Though all the mutations are located within TNT1, they have widely different phenotypes clinically and biophysically. Using a complete atomistic model of the cTn–Tm complex, we identify mechanisms by which the effects of TNT1 mutations propagate to the cTn core and site II of cTnC, where calcium binding and dissociation occurs. We find that mutations in TNT1 alter the flexibility of TNT1, which is inversely proportional to the cooperativity of calcium activation of the thin filament. Further, we identify a pathway of propagation of structural and dynamic changes from TNT1 to site II of cTnC, including TNT1, cTnT linker, I-T arm, regulatory domain of cTnI, the D-E linker of cTnC, and site II cTnC. Mutationally induced changes at site II of cTnC alter calcium coordination that corresponds to biophysical measurements of calcium sensitivity. Finally, we compare this pathway of mutational propagation with that of the calcium activation of the thin filament and find that they are identical but opposite in direction. PMID:22579624

Manning, Edward P.; Tardiff, Jil C.; Schwartz, Steven D.

2012-01-01

120

The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916.  

PubMed Central

The Bacteroides mobilizable transposon Tn4555 is a 12.2-kb molecule that encodes resistance to cefoxitin. Conjugal transposition is hypothesized to occur via a circular intermediate and is stimulated by coresident tetracycline resistance elements and low levels of tetracycline. In this work, the ends of the transposon were identified and found to consist of 12-bp imperfect inverted repeats, with an extra base at one end. In the circular form, the ends were separated by a 6-bp "coupling sequence" which was associated with either the left or the right transposon terminus when the transposon was inserted into the chromosome. Tn4555 does not duplicate its target site upon insertion. Using a conjugation-based transposition assay, we showed that the coupling sequence originated from 6 bases of genomic DNA flanking either side of the transposon prior to excision. Tn4555 preferentially transposed into a 589-bp genomic locus containing a 207-bp direct repeat. Integration occurred before or after the repeated sequence, with one integration site between the two repeats. These observations are consistent with a transposition model based on site-specific recombination. In the bacteriophage lambda model for site-specific recombination, the bacteriophage recombines with the Escherichia coli chromosome via a 7-bp "crossover" region. We propose that the coupling sequence of Tn4555 is analogous in function to the crossover region of lambda but that unlike the situation in lambda, recombination occurs between regions of nonhomologous DNA. This ability to recombine into divergent target sites is also a feature of the gram-positive bacterial transposon Tn916. PMID:9098073

Tribble, G D; Parker, A C; Smith, C J

1997-01-01

121

Tn6249, a New Tn6162 Transposon Derivative Carrying a Double-Integron Platform and Involved with Acquisition of the blaVIM-1 Metallo-?-Lactamase Gene in Pseudomonas aeruginosa.  

PubMed

The In70.2 integron platform appears to be a conserved structure involved in the dissemination of the blaVIM-1 metallo-?-lactamase gene in Pseudomonas aeruginosa. The genetic context of the In70.2 integron platform from P. aeruginosa VR-143/97, the VIM-1-producing index strain isolated in Italy in 1997, was fully characterized by a next-generation sequencing approach refined by conventional sequencing. The In70.2 integron platform from VR-143/97 was found to be associated with a defective Tn402-like transposon inserted into the urf2 gene of a Tn3 family transposon of an original structure, named Tn6249, which also carried a partially deleted mer operon and an In90 integron platform in a tail-to-tail orientation. Tn6249 was inserted into a PACS171b-like genomic island, which was in turn inserted into the endA gene of the Pseudomonas chromosomal backbone. Tn6249 showed a similar structure and a conserved location with respect to that of Tn6060, a Tn3 family transposon associated with In70.2 and carrying a double-integron platform, which was detected in a VIM-1-producing P. aeruginosa strain isolated in Australia in 2008. Both Tn6249 and Tn6060 are apparently derived from Tn6162, a mercury resistance transposon carrying an integron platform, which was found in P. aeruginosa isolates from different geographic locations. The conservation of the genetic context of Tn6249 and Tn6060 suggests an in situ evolution of these elements after the insertion of a Tn6162-like ancestor into the PACS171b-like genomic island (GI) present in the genome of a successful widespread P. aeruginosa clonal lineage. PMID:25547348

Di Pilato, Vincenzo; Pollini, Simona; Rossolini, Gian Maria

2015-03-01

122

A newly generated functional antibody identifies Tn antigen as a novel determinant in the cancer cell–lymphatic endothelium interaction  

PubMed Central

Malignant transformation of epithelial cells is frequently associated with the alteration of glycosylation pathways. Tn is a common tumor-associated carbohydrate antigen present in 90% of human carcinomas and its expression correlates with metastatic potential and poor prognosis. Despite its relevance, the functional role of Tn in tumor biology has not been firmly established probably for the lack of appropriate experimental tools. Our aims were to produce highly reactive monoclonal antibodies against Tn making use of synthetically produced Tn and to test their usefulness for in vivo imaging as well as to define their potential functional activity in tumor cell spread. We immunized mice with Tn clustered on cationized BSA and screened the positive hybridomas with Tn-biotinylated alginate. Enzyme-linked immuno sorbent assay and immunofluorescence assays revealed that the most reactive anti-Tn IgM mAb (2154F12A4) selectively recognized Tn on the MCF7 breast cancer cell line since its binding to the cell membrane was completely abolished by preincubation with purified Tn. Importantly, QDot 800-conjugated mAb injected in MCF7-tumor bearing mice specifically bound to primary tumor lesions as well as to metastases in lymph nodes. In addition, this mAb was able to inhibit cancer cell adhesion to lymphatic endothelium suggesting a novel involvement of Tn in the lymphatic dissemination of cancer cells and hypothesizing future applications in inhibiting lymphatic metastases. PMID:19528665

Danussi, Carla; Coslovi, Anna; Campa, Cristiana; Mucignat, Maria T; Spessotto, Paola; Uggeri, Fulvio; Paoletti, Sergio; Colombatti, Alfonso

2009-01-01

123

Fis plays a role in Tn5 and IS50 transposition.  

PubMed Central

The Fis (factor for inversion stimulation) protein of Escherichia coli was found to influence the frequency of transposon Tn5 and insertion sequence IS50 transposition. Fis stimulated both Tn5 and IS50 transposition events and also inhibited IS50 transposition in Dam-bacteria. This influence was not due to regulation by Fis of the expression of the Tn5 transposition proteins. We localized, by DNase I footprinting, one Fis site overlapping the inside end of IS50 and give evidence to strongly suggest that when Fis binds to this site, IS50 transposition is inhibited. The Fis site at the inside end overlaps three Dam GATC sites, and Fis bound efficiently only to the unmethylated substrate. Using a mobility shift assay, we also identified another potential Fis site within IS50. Given the growth phase-dependent expression of Fis and its differential effect on Tn5 versus IS50 transposition in Dam-bacteria, we propose that the high levels of Fis present during exponential growth stimulate transposition events and might bias those events toward Tn5 and away from IS50 transposition. Images PMID:1320613

Weinreich, M D; Reznikoff, W S

1992-01-01

124

Tn5 transposase and tagmentation procedures for massively scaled sequencing projects  

PubMed Central

Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications. PMID:25079858

Picelli, Simone; Björklund, Åsa K.; Reinius, Björn; Sagasser, Sven; Winberg, Gösta

2014-01-01

125

[Variations of annual load of TN and TP in the deep bay watershed, Shenzhen].  

PubMed

The empirical coefficient of sewage disposal, export coefficient model and mean concentration method were respectively used to estimate variations of annual load TN and TP from Shenzhen and Hong Kong areas in the Deep Bay Watershed from 1986 to 2011. The results showed that, the annual average loads of TN and TP were 10 388.2 t, 10 727.9 t, 10 937.3 t, and 2 694.5 t, 1 929.2 t, 1388.7 t, respectively in the whole watershed during three periods, 80s, 90s and years after 2000. With the rapid development of society, economy and the urbanization, annual pollution loading of TN and TP in Shenzhen area showed an obviously increase, 4373.6 t and 195.9 t, by 261.0% and 64.2% for point source, and 1067.2 t and 151.0 t, by 63.4% and 84.9% for non-point source, respectively. Non-point source with high pollution load was mainly caused by the expanding of land for construction and roads. The contribution ratios of TN and TP from Shenzhen area increased from 42.4% and 27.0% to 85.1% and 75.2%. Annual loads of TN and TP in Hong Kong area decreased 3 028.5 t and 1 031.5 t, by 66.3% and 79.0% reduced. PMID:25639083

Zhao, Chen-Chen; Zhang, Shi-Yan; Mao, Xian-Zhong

2014-11-01

126

TheBacteroidesMobilizable Transposon Tn4555Integrates by a Site-Specific Recombination Mechanism Similar to That of the Gram-Positive Bacterial Element Tn916  

Microsoft Academic Search

The Bacteroides mobilizable transposon Tn4555 is a 12.2-kb molecule that encodes resistance to cefoxitin. Conjugal transposition is hypothesized to occur via a circular intermediate and is stimulated by coresident tetracycline resistance elements and low levels of tetracycline. In this work, the ends of the transposon were identified and found to consist of 12-bp imperfect inverted repeats, with an extra base

GENA D. TRIBBLE; ANITA C. PARKER; JEFFREY SMITH

1997-01-01

127

The crystal structure of a plant lectin in complex with the Tn antigen.  

PubMed

The structure of the tetrameric Vicia villosa isolectin B4 (VVLB4) in complex with a cancer antigen, the Tn glycopeptide (GalNAc-O-Ser), was determined at 2.7 A resolution. The N-acetylgalactoside moiety of the ligand binds to the primary combining site of VVLB4 in a similar way as observed for other Gal/GalNAc-specific plant lectins. The amino acid moiety of the Tn antigen is largely exposed to the solvent and makes few contacts with the protein. The structure of the complex provides a framework to understand the differences in the strength of VVLB4 binding to different sugars and emphasizes the role of a single protein residue, Tyr127, as a structural determinant of Tn-binding specificity. PMID:12586347

Babino, Alvaro; Tello, Diana; Rojas, Adriana; Bay, Sylvie; Osinaga, Eduardo; Alzari, Pedro M

2003-02-11

128

4. Photocopy of photograph showing the corn crib as it ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Photocopy of photograph showing the corn crib as it looked on May 21, 1936. This structure was also used as a gear shed. Immediately to the right of the shed is Hettie Walker and an unidentified family member or friend. The original photograph was taken by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8091. - Walker Family Farm, Corn Crib, Gatlinburg, Sevier County, TN

129

Snohomish County Biodiesel Project  

SciTech Connect

Snohomish County in western Washington State began converting its vehicle fleet to use a blend of biodiesel and petroleum diesel in 2005. As prices for biodiesel rose due to increased demand for this cleaner-burning fuel, Snohomish County looked to its farmers to ���¢��������grow���¢������� this fuel locally. Suitable seed crops that can be crushed to extract oil for use as biodiesel feedstock include canola, mustard, and camelina. The residue, or mash, has high value as an animal feed. County farmers began with 52 acres of canola and mustard crops in 2006, increasing to 250 acres and 356 tons in 2008. In 2009, this number decreased to about 150 acres and 300 tons due to increased price for mustard seed.

Terrill Chang; Deanna Carveth

2010-02-01

130

In-site interaction evaluation of Tn density by inhibition/competition assays.  

PubMed

The tumor-associated structure N-acetyl-galactosamine-O-Ser/Thr (Tn antigen), which is overexpressed in various tumor cell types, notably of the breast, ovary and colon, is an interesting determinant that is useful for cancer diagnosis and follow-up. The aim of this research was to study different assay strategies in order to determine the most sensitive system for further application in epitope characterization and binding assessment. The tetrameric isolectin obtained from Vicia villosa seeds (VVLB(4)) shows high affinity for the tumor-associated structure. A monoclonal antibody against VVLB(4), MabVV(34), was generated, and the interaction between MabVV(34) and VVLB(4) was studied by means of binding and inhibition assays. Several synthetic peptides (10 amino acid sequences) designed from the amino acid sequence of VVLB(4) and obtained from trypsin digestion were tested to determine which amino acids were involved in the interaction between MabVV(34) and VVLB(4). The further unraveling of this epitope was investigated by inhibition using designed synthetic peptides as well as mixtures mimicking variable density effect. Under the experimental circumstances, MabVV(34) was able to inhibit the binding of VVLB(4) to Tn. Two of the four peptide sequences assayed showed better inhibition properties. Finally, mixtures containing these selected sequences allowed the evaluation of binding and inhibition as a function of Tn density. We conclude that the present study facilitates the further development of a specific Tn marker and may contribute to the development of Tn-like radiolabelled peptides or Tn-specific radiolabelled fragments providing a highly selective tool for cancer diagnosis and treatment. This strategy may contribute to characterize the new generation of radiopharmaceuticals for diagnosis and therapy based on biomolecules like antibodies, fragments or peptides, whose application is directly guided by their specific molecular recognition. PMID:20447557

Robles, Ana; Medeiros, Andrea; Berois, Nora; Balter, Henia S; Pauwels, Ernest K; Osinaga, Eduardo

2010-05-01

131

Novel Tn4371-ICE like element in Ralstonia pickettii and Genome mining for comparative elements  

PubMed Central

Background Integrative Conjugative Elements (ICEs) are important factors in the plasticity of microbial genomes. An element related to the ICE Tn4371 was discovered during a bioinformatic search of the Ralstonia pickettii 12J genome. This element was analysed and further searches carried out for additional elements. A PCR method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight Ralstonia pickettii and Ralstonia insidiosa strains were analysed for the presence of the element. Results Comparative sequence analysis of bacterial genomes has revealed the presence of a number of uncharacterised Tn4371-like ICEs in the genomes of several ? and ?- Proteobacteria. These elements vary in size, GC content, putative function and have a mosaic-like structure of plasmid- and phage-like sequences which is typical of Tn4371-like ICEs. These elements were found after a through search of the GenBank database. The elements, which are found in Ralstonia, Delftia, Acidovorax, Bordetella, Comamonas, Acidovorax, Congregibacter, Shewanella, Pseudomonas Stenotrophomonas, Thioalkalivibrio sp. HL-EbGR7, Polaromonas, Burkholderia and Diaphorobacter sp. share a common scaffold. A PCR method was designed (based on the Tn4371- like element detected in the Ralstonia pickettii 12J genome) to detect and characterise new elements of this type. Conclusion All elements found in this study possess a common scaffold of core genes but contain different accessory genes. A new uniform nomenclature is suggested for ICEs of the Tn4371 family. Two novel Tn4371-like ICE were discovered and characterised, using the novel PCR method described in two different isolates of Ralstonia pickettii from laboratory purified water. PMID:19941653

2009-01-01

132

Persistent Tn polyagglutination syndrome during febrile neutropenia: a case report and review of the literature  

PubMed Central

Introduction Tn polyagglutination syndrome is a rare disorder that has been reported on only a few occasions in the literature, and, to the best of our knowledge, never before in the context of febrile neutropenia. Case presentation We report the case of a 26-year-old Caucasian woman who presented to our emergency department complaining of a persistent fever over the previous three days. She had a history of long-standing refractory pancytopenia with multi-lineage dysplasia and severe neutropenia, but she had rarely experienced infection. The results of a physical examination and multiple laboratory tests were unremarkable. While investigating the possible causes of the refractory, long-standing pancytopenia, the possibility of a polyagglutinable state was suggested. Blood samples were sent to the laboratory for an analysis of mixed-field seed lectin agglutination assay. A serum lectin panel confirmed the final diagnosis of Tn-activation. Conclusions We should include Tn-activation in our differential whenever we encounter cases of refractory long-standing idiopathic cytopenias and inconclusive bone marrow results displaying multi-lineage dysplasia. Novel genetic techniques have recently revealed the interesting pathophysiology of this phenomenon. The recognition and inclusion of Tn polyagglutination syndrome in our differential diagnoses has important clinical implications, given its main associated features, such as severe thrombocytopenia and neutropenia, which are usually linked to a benign clinical course and prognosis. Increased awareness of the polyagglutinable disorders will potentially decrease the need for invasive and costly medical interventions and also raises the need for monitoring of this specific sub-set of patients. In addition, the study of the expression and implications of Tn, and other similar antigens, offers a fascinating perspective for the study of its role in the diagnosis, prognosis and immunotherapy of solid tumors and hematological malignancies. The infrequency with which Tn polyagglutination syndrome is encountered, its clinical features and its pathophysiology make it a formidable diagnostic challenge. PMID:21235740

2011-01-01

133

Jasper County Comprehensive Plan Adoption Draft  

E-print Network

Jasper County Comprehensive Plan Adoption Draft Comprehensive Plan Jasper County, IN #12;#12;Table Mandate.......................................vii Jasper County's Fulfillment of the Mandate of Major Needs for Jasper County ...............5 Additional Opportunities

134

Quantitative assessment of Tn antigen in breast tissue micro-arrays using CdSe aqueous quantum dots.  

PubMed

In this study, we examined the use of CdSe aqueous quantum dots (AQDs) each conjugated to three streptavidin as a fluorescent label to image Tn antigen expression in various breast tissues via a sandwich staining procedure where the primary monoclonal anti-Tn antibody was bound to the Tn antigen on the tissue, a biotin-labeled secondary antibody was bound to the primary anti-Tn antibody, and finally the streptavidin-conjugated AQDs were bound to the biotin on the secondary antibody. We evaluated the AQD staining of Tn antigen on tissue microarrays consisting of 395 cores from 115 cases including three tumor cores and one normal-tissue core from each breast cancer case and three tumor cores from each benign case. The results indicated AQD-Tn staining was positive in more than 90% of the cells in the cancer cores but not the cells in the normal-tissue cores and the benign tumor cores. As a result, AQD-Tn staining exhibited 95% sensitivity and 90% specificity in differentiating breast cancer against normal breast tissues and benign breast conditions. These results were better than the 90% sensitivity and 80% specificity exhibited by the corresponding horse radish peroxidase (HRP) staining using the same antibodies on the same tissues and those of previous studies that used different fluorescent labels to image Tn antigen. In addition to sensitivity and specificity, the current AQD-Tn staining with a definitive threshold was quantitative. PMID:24411673

Au, Giang H T; Mejias, Linette; Swami, Vanlila K; Brooks, Ari D; Shih, Wan Y; Shih, Wei-Heng

2014-03-01

135

Tn125-Related Acquisition of blaNDM-Like Genes in Acinetobacter baumannii  

PubMed Central

A multidrug-resistant Acinetobacter baumannii isolate recovered from a patient hospitalized in Switzerland after a transfer from Serbia produced the NDM-1 carbapenemase. The blaNDM-1 gene was part of a chromosomally located Tn125 composite transposon bracketed by two copies of the same insertion sequence, ISAba125. This transposon was also associated with the acquisition and expression of the blaNDM-2 gene in an A. baumannii isolate in Germany. Tn125 appears to be the main vehicle for dissemination of blaNDM genes in that species. PMID:22143526

Poirel, Laurent; Bonnin, Rémy A.; Boulanger, Anne; Schrenzel, Jacques; Kaase, Martin

2012-01-01

136

Petroleum County Secondary Data Analysis  

E-print Network

Petroleum County Secondary Data Analysis July 23, 2012 1 Community Health Data, MT Dept American Diabetes Association (2012) Region 3 (South Central) ­ Judith Basin, Fergus, Petroleum* #12; Petroleum County Secondary Data Analysis July 23, 2012 2 Socioeconomic Measures1

Maxwell, Bruce D.

137

Wasp Watchers Albany County  

E-print Network

{ Wasp Watchers Albany County Finding the Wasp that hunts the Emerald Ash Borer #12;Cerceris fumipennis is a solitary ground nesting wasp. The female wasp stocks her nest with Buprestid beetles ,this as 80% of the Cerceris fumipennis colonies daily catch. · This wasp is very wide spread in the United

Walter, M.Todd

138

Meigu County Yi Tone.  

ERIC Educational Resources Information Center

One dialect of Yi spoken in Meigu County in the southern part of China's Sichuan Province is analyzed for its tone patterns, based on data provided by a bilingual native speaker. Consonant and vowel inventories are provided. Three contrastive tones are found. One has three allophones, which are conditioned by the preceding tone. Tonal allophony is…

Eatough, Andy

139

STATE/COUNTY BORDERS  

EPA Science Inventory

This data is available for the entire region. The level of detail is primarily suited for region/state/county/basin sized maps. The borders are not accurate enough for small areas like sub-basins and site areas. We use this coverage for general backgrounds and borders only. T...

140

IS10/Tn10 transposition efficiently accommodates diverse transposon end configurations.  

PubMed Central

Transposon Tn10 and its component insertion sequence IS10 move by non-replicative transposition. We have studied the array of reaction intermediates and products in a high efficiency in vitro IS10/Tn10 transposition reaction. Synapsis of two transposon ends, followed by cleavage and strand transfer, can occur very efficiently irrespective of the relative locations and orientations of the two ends. The two participating ends can occur in inverted or direct orientation on the same molecule or, most importantly, on two different molecules. This behavior contrasts sharply with that of Mu, in which transposition is strongly biased in favor of inverted repeat synapsis. Mechanistically, the absence of discrimination amongst various end configurations implies that the architecture within the IS10/Tn10 synaptic complex is relatively simple, i.e. lacking any significant intertwining of component DNA strands. Biologically these observations are important because they suggest that the IS10 insertion sequence module has considerable flexibility in the types of DNA rearrangements that it can promote. Most importantly, it now seems highly probable that a single non-replicative IS10 element can promote DNA rearrangements usually attributed to replicative transposition, i.e. adjacent deletions and cointegrates, by utilizing transposon ends on two sister chromosomes. Other events which probably also contribute to the diversity of IS10/Tn10-promoted rearrangements are discussed. Images PMID:8890185

Chalmers, R M; Kleckner, N

1996-01-01

141

76 FR 4147 - Putnam-Cumberland, TN-Improve Power Supply  

Federal Register 2010, 2011, 2012, 2013, 2014

...AUTHORITY Putnam-Cumberland, TN--Improve Power Supply AGENCY: Tennessee Valley Authority...environmental effects of proposed electrical power supply improvements in the Putnam and Cumberland...ensure the reliable transmission of electric power to meet increasing power demands in...

2011-01-24

142

INTRODUCTION Tenascin-C (TN-C), a large glycoprotein of the extracellular  

E-print Network

candidates for this enhancement of binding. Two heparin-binding sites have been identified in TN-C. A heparin expression proteins (Aukhil et al., 1993; Weber et al., 1995). The second heparin-binding site is in TNfbg embryo fibroblasts can adhere to TNfbg, and this adhesion was inhibited by exogenous heparin

Erickson, Harold P.

143

Defining characteristics of Tn5 Transposase non-specific DNA binding  

PubMed Central

While non-specific DNA plays a role in target localization for many recombinases, transcription factors and restriction enzymes, the importance of non-specific DNA interactions for transposases has not been investigated. Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest how they stabilize the Tnp and modulate Tnp localization of the 19 bp Tnp recognition end sequences (ESes). DNA protection assays indicate that full-length Tnp interacts efficiently with supercoiled DNA that does not contain ESes. These interactions significantly prolong the lifetime of Tnp, in vitro. The balance between non-specific DNA bound and free Tnp is affected by DNA topology, yet, intermolecular transfer of active Tnp occurs with both supercoiled and linear non-specific DNA. Experiments with substrates of varying lengths show that Tn5 Tnp can utilize non-specific DNA to facilitate localization of an intramolecular ES over distances less than 464 bp. Finally, synaptic complex formation is inhibited in the presence of increasing concentrations of supercoiled and linear pUC19. These experiments strongly suggest that Tn5 Tnp has a robust non-specific DNA binding activity, that non-specific DNA modulates ES sequence localization within the global DNA, most likely through a direct transfer mechanism, and that non-specific DNA binding may play a role in the cis bias manifested by Tn5 transposition. PMID:16717287

Steiniger, Mindy; Adams, Christian D.; Marko, John F.; Reznikoff, William S.

2006-01-01

144

A Multicomponent System Is Required for Tetracycline-Induced Excision of Tn4555  

Microsoft Academic Search

Bacteroides spp. are the predominant organisms in the intestinal tract, and they also are important oppor- tunistic pathogens. Antibiotic therapy of Bacteroides infections often is complicated by the prevalence of drug-resistant organisms which acquire resistance genes from a variety of mobile genetic elements including conjugative transposons (CTns) and mobilizable transposons (MTns). Tn4555 is an MTn that encodes -lactam resistance, and

Anita C. Parker; C. Jeffrey Smith

2004-01-01

145

ERDC/TN APCRP-EA-22 Management of Half Moon Lake,  

E-print Network

ERDC/TN APCRP-EA-22 May 2010 Management of Half Moon Lake, Wisconsin, for Improved Native Submersed that result in poor light penetration and limited colonizable macrophyte habitat. Half Moon Lake Half Moon Lake is shallow and supports a native submersed macrophyte community, poor underwater light

US Army Corps of Engineers

146

Characterization of glucansucrase and dextran from Weissella sp. TN610 with potential as safe food additives.  

PubMed

Pear-derived Weissella sp. TN610 produced extracellular glycosyltransferase activity responsible for the synthesis of soluble exopolysaccharide from sucrose. Acid and dextranase-catalyzed hydrolysis revealed that the synthesized polymer was a glucan. According to (1)H and (13)C NMR analysis, the glucan produced by TN610 was a linear dextran made of 96% ?-(1?6) and 4% ?-(1?3) linkages. Zymogram analysis confirmed the presence of a unique glucansucrase of approximately 180 kDa in the cell-free supernatant from TN610. The crude enzyme, optimally active at 37°C and pH 5, has promising potential for application as a food additive since it catalyzes dextran synthesis in sucrose-supplemented milk, allowing its solidification. A 4257-bp product corresponding to the mature glucansucrase gene was amplified by PCR from TN610. It encoded a polypeptide of 1418 residues having a calculated molecular mass of 156.089 kDa and exhibiting 96% and 95% identity with glucansucrases from Lactobacillus fermentum Kg3 and Weissella cibaria CMU, respectively. PMID:23046923

Bejar, Wacim; Gabriel, Valérie; Amari, Myriam; Morel, Sandrine; Mezghani, Monia; Maguin, Emmanuelle; Fontagné-Faucher, Catherine; Bejar, Samir; Chouayekh, Hichem

2013-01-01

147

A correlation between specific heat and the ratio TK\\/TN in magnetic Kondo lattices  

Microsoft Academic Search

Specific heat results on magnetic Ce or Yb Kondo systems are presented and described within the approach based on molecular field calculations for the S=1\\/2 resonant level model. Within this framework, a close relationship between the specific heat jump, deltaC, at the ordering temperature and the ratio between the two characterictic temperatures TK and TN is demonstrated.

M. J. Besnus; A. Braghta; N. Hamdaoui; A. Meyer

1992-01-01

148

Approved for public release; distribution is unlimited. ERDC TN-DOER-E32  

E-print Network

removed. A single magnetic drive pump in each aquarium recirculates water, providing energy to maintain sediment in suspension and aerating the water. #12;ERDC TN-DOER-E32 August 2012 2 Figure 1. Schematic cone-bottom polyethylene tank and is continuously mixed by recirculating it using a double diaphragm

US Army Corps of Engineers

149

Wetlands Regulatory Assistance Program ERDC TN-WRAP-12-1 August 2012  

E-print Network

Wetlands Regulatory Assistance Program ERDC TN-WRAP-12-1 August 2012 Approved for public release; distribution is unlimited. Updating Regional Supplements to the Corps of Engineers Wetland Delineation Manual by Jacob F. Berkowitz PURPOSE: Regional supplements to the Corps of Engineers Wetland Delineation Manual

US Army Corps of Engineers

150

Fusion Power Associates Annual Meeting and Symposium, Oak Ridge, TN, 4-5 December 2007 Gary Johnson  

E-print Network

1 Fusion Power Associates Annual Meeting and Symposium, Oak Ridge, TN, 4-5 December 2007 Gary Era Status of ITERStatus of ITER #12;2 Fusion Power Associates Annual Meeting and Symposium, Oak Ridge · Technical Status · Summary #12;3 Fusion Power Associates Annual Meeting and Symposium, Oak Ridge, TN, 4

151

78 FR 43827 - Irish Potatoes Grown in Modoc and Siskiyou Counties, California, and in All Counties in Oregon...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Siskiyou Counties, California, and in All Counties in Oregon, Except Malheur County...Siskiyou Counties, California, and in all counties in Oregon, except Malheur County...at: http://www.regulations.gov. All comments submitted in response to this...

2013-07-22

152

Purchasing in Texas Counties.  

E-print Network

delivery in the most economical manner. Perhaps the manner in which delivery is taken deserves addi- tional attention. For example, this study indicates that all counties taking delivery of gasoline in tank wagon lots, can secure approximately the same... recog- nizes that some one person or board should he responsible for a high per- centage of the purchasing. Otherwise each employee must become an ex- pert in order to secure good prices. Further, such a system recognizes that bids must be secured...

Hervey, E. J.; Bradshaw, H. C.

1944-01-01

153

High-resolution structure of a new Tn antigen-binding lectin from Vatairea macrocarpa and a comparative analysis of Tn-binding legume lectins.  

PubMed

Plant lectins have been studied as histological markers and promising antineoplastic molecules for a long time, and structural characterization of different lectins bound to specific cancer epitopes has been carried out successfully. The crystal structures of Vatairea macrocarpa (VML) seed lectin in complex with GalNAc-?-O-Ser (Tn antigen) and GalNAc have been determined at the resolution of 1.4? and 1.7?, respectively. Molecular docking analysis of this new structure and other Tn-binding legume lectins to O-mucin fragments differently decorated with this antigen provides a comparative binding profile among these proteins, stressing that subtle alterations that may not influence monosaccharide binding can, nonetheless, directly impact the ability of these lectins to recognize naturally occurring antigens. In addition to the specific biological effects of VML, the structural and binding similarities between it and other lectins commonly used as histological markers (e.g., VVLB4 and SBA) strongly suggest VML as a candidate tool for cancer research. PMID:25499445

Sousa, Bruno Lopes; Silva Filho, José Caetano; Kumar, Prashant; Pereira, Ronniery Ilário; ?yskowski, Andrzej; Rocha, Bruno Anderson Matias; Delatorre, Plínio; Bezerra, Gustavo Arruda; Nagano, Celso Shiniti; Gruber, Karl; Cavada, Benildo Sousa

2015-02-01

154

78 FR 29202 - Environmental Impact Statement: Grand Forks County, North Dakota and Polk County, Minnesota  

Federal Register 2010, 2011, 2012, 2013, 2014

...Statement: Grand Forks County, North Dakota and Polk County, Minnesota AGENCY...project in Grand Forks County, North Dakota and Polk County, Minnesota. FOR...Federal Highway Administration, North Dakota Division Office, 1471...

2013-05-17

155

77 FR 72968 - Revisions to the California State Implementation Plan, for Imperial County, Placer County and...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Placer County and Ventura County Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD)...

2012-12-07

156

77 FR 73005 - Revisions to the California State Implementation Plan, Imperial County, Placer County, and...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Placer County, and Ventura County Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD)...

2012-12-07

157

Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp  

PubMed Central

Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we demonstrated the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome was detected at a Tn7 attachment (attTn7) site located downstream of glmS1. Furthermore, using a newly constructed vector pBBR1FLP2 containing the FLP recombinase for site-specific excision of the sequence between the FLP recognition target (FRT) sites, and a sacB counter selection marker, an unmarked mini-Tn7 insertion mutant was created. Mini-Tn7 insertion did not affect bacterial virulence on the tested plant. The mini-Tn7 and FLP-FRT systems also work well in X. oryzae pv. oryzae. PMID:19659730

Jittawuttipoka, Thichakorn; Buranajitpakorn, Sarinya; Fuangthong, Mayuree; Schweizer, Herbert P.; Vattanaviboon, Paiboon; Mongkolsuk, Skorn

2009-01-01

158

Dr John T Ammons "Tom" or "Doc" to his family and friends 68 years old of Louisville, TN passed away at  

E-print Network

Dr John T Ammons "Tom" or "Doc" to his family and friends 68 years old of Louisville, TN passed of Tennessee. He is survived by his Wife: Tatiana Karpinets of Louisville, TN, Her Son and Daughter in law

Tennessee, University of

159

Dynamics of the C-Terminal Region of TnI in the Troponin Complex in Solution  

PubMed Central

The determination of crystal structures of the troponin complex (Takeda et al. 2003. Nature. 424:35–41; Vinogradova et al. 2005. Proc. Natl. Acad. Sci. USA. 102:5038–5043) has advanced knowledge of the regulation of muscle contraction at the molecular level. However, there are domains important for actin binding that are not visualized. We present evidence that the C-terminal region of troponin I (TnI residues 135–182) is flexible in solution and has no stable secondary structure. We use NMR spectroscopy to observe the backbone dynamics of skeletal [2H, 13C, 15N]-TnI in the troponin complex in the presence of Ca2+ or EGTA/Mg2+. Residues in this region give stronger signals than the remainder of TnI, and chemical shift index values indicate little secondary structure, suggesting a very flexible region. This is confirmed by NMR relaxation measurements. Unlike TnC and other regions of TnI in the complex, the C-terminal region of TnI is not affected by Ca2+ binding. Relaxation measurements and reduced spectral density analysis are consistent with the C-terminal region of TnI being a tethered domain connected to the rest of the troponin complex by a flexible linker, residues 137–146, followed by a collapsed region with at most nascent secondary structure. PMID:16415057

Blumenschein, Tharin M. A.; Stone, Deborah B.; Fletterick, Robert J.; Mendelson, Robert A.; Sykes, Brian D.

2006-01-01

160

Digital Atlas of Texas Counties  

NSDL National Science Digital Library

Created by the Center for Geosptial Technology at Texas Tech University, this digital atlas is a fantastic find. It brings together information about all of Texas's counties, including satellite relief maps and data sets about the roads, rivers, lakes, and other features in each area. Visitors can use the interactive map to click on a county of interest, or use the drop down menu to select a region. Once users select a county, they can download information about it for future use. The site also contains a number of Featured Links to resources from the U.S. Census Bureau, the Texas Association of Counties, and several tourism agencies.

161

Unique insertion site of Tn7 in the E. coli chromosome  

Microsoft Academic Search

Among transposable elements, transposon Tn7 is unusual in that it inserts into a particular site of the Escherichia coli chromosome with high efficiency1,2 and with a unique orientation2. Most other transposable elements in prokaryotes are capable of translocating from and to a variety of sites in bacteriophage and plasmid DNA as well as in the chromosome3. We have previously shown

Conrad Lichtenstein; Sydney Brenner

1982-01-01

162

Morphological and Functional Diversity of Ectomycorrhizal Fungi on Roan Mountain (NC\\/TN)  

Microsoft Academic Search

A comparison of ectomycorrhizal morphotypes and hypogeous fungi (truffles and false-truffles) in northern hardwood and spruce-fir forests on Roan Mountain (NC\\/TN) was performed to increase our knowledge of the fungal communities in the Southern Appalachian high elevation forests. These forests are home to an endangered subspecies and mycophagist, the Carolina northern flying squirrel (Glaucomys sabrinus coloratus), as well as the

Claire Bird; Coleman McCleneghan

2005-01-01

163

ROLE OF NA+ IN TRANSPORT OF HG2+ AND INDUCTION OF THE TN21 "MER" OPERON  

EPA Science Inventory

The effects of sodium ions on the uptake of Hg 2 + and induction of the TN21 mer operon were studied using E. coli HMS174 harboring the reporter plasmids pRB28 and pOS14. lasmid pRB28 carries merRT' and pOS14 carries merRTPC of the mer operon, both cloned upstream of a promoterle...

164

Genechip-detecting mutations in exon 8 in cTnI gene associated with FHCM  

NASA Astrophysics Data System (ADS)

As the rate of gene discovery accelerates, more efficient methods are needed to analyze genes in human tissues. Genechip, a kind of new device, is composed of DNA probes immobilized on a solid substrate. With the advantage of the high throughput information, genechip has become one of the best solutions to detect and analyse the mutations in genes. Hypertrophic cardiomyopathy (HCM), the most common cause of the sudden death in the young, is one of the diseases damaging people health most badly. It is an autosomal dominant disease. More than 55% of the HCM patients are genetic. The mutations of exon 8 in the Cardiac troponin I (cTnI) gene are closely associated with Family Hypertrophic Cardiomyopathy (FHCM). Our purpose is to perform the assay of the mutations in exon 8 in cTnI gene based on the genechip theory and technology. Special probes were designed to fabricate the genechip to detect the mutations in cTnI gene simultaneously. We designed two oligonucleotide sequences 5"-end labeled with fluorescein, one simulating wild-type and the other simulating mutant. We mixed oligonucleotide I and II together to simulate heterozygote. After optimizing the hybridization protocols, the fabricated genechip can detect the mutations in exon 8 in cTnI gene with relative high sensitivity and specificity. When applying the fabricated genechip to detect the target DNA sequence, we found that the fully complementary probe gave a fluorescent signal almost 50% stronger than that of the one base mismatched one, which is in accordance with the result from theoretic estimate. It is believed that an applicable special genechip can be developed for investigating and diagnosing FHCM after further improvement.

Zhang, Yuanying; He, Nongyue; Guo, Huishi; Yang, Di; Wan, Wenhui; Bian, Zhiping; Zhang, Jinan

2005-01-01

165

Tn5706, a Transposon-Like Element from Pasteurella multocida Mediating Tetracycline Resistance  

PubMed Central

The 4,378-bp putative tetracycline resistance transposon Tn5706 of Pasteurella multocida consists of an internal tet(H)-tetR resistance gene region which is flanked by almost identical insertion elements, IS1596 and IS1597. Two reading frames for proteins of 70 and 228 amino acids were detected in each of these insertion sequence elements. The 228-amino-acid protein revealed homology to transposase proteins of gram-positive bacteria. PMID:9687418

Kehrenberg, Corinna; Werckenthin, Christiane; Schwarz, Stefan

1998-01-01

166

A Multicomponent System Is Required for Tetracycline-Induced Excision of Tn4555  

PubMed Central

Bacteroides spp. are the predominant organisms in the intestinal tract, and they also are important opportunistic pathogens. Antibiotic therapy of Bacteroides infections often is complicated by the prevalence of drug-resistant organisms which acquire resistance genes from a variety of mobile genetic elements including conjugative transposons (CTns) and mobilizable transposons (MTns). Tn4555 is an MTn that encodes ?-lactam resistance, and it is efficiently mobilized by the Bacteroides CTns via a tetracycline (TET)-inducible mechanism. In this study a model system with CTn341 and a Tn4555 minielement was used to examine Tn4555 excision from the chromosome. Using PCR and mobilization assays it was established that excision was stimulated by TET in the presence of CTn341. In order to determine which Tn4555 genes were required for excision, int, tnpA, tnpC, xis, and mobA mutants were examined. The results indicated that int plus two additional genes, tnpC and xis, were required for optimal excision. In addition, there was no requirement for the mobA gene, as had been shown for another MTn, NBU1. The Xis protein sequence is related to a family of plasmid excisionases, but the TnpC gene product did not match anything in the sequence databases. Evidence also was obtained that suggested that Xis is involved in the control of TET-induced excision and in control of mobilization by CTn341. Overall, these results indicate that excision of MTns is a complex process that requires multiple gene products. ? PMID:14702313

Parker, Anita C.; Smith, C. Jeffrey

2004-01-01

167

A multicomponent system is required for tetracycline-induced excision of Tn4555.  

PubMed

Bacteroides spp. are the predominant organisms in the intestinal tract, and they also are important opportunistic pathogens. Antibiotic therapy of Bacteroides infections often is complicated by the prevalence of drug-resistant organisms which acquire resistance genes from a variety of mobile genetic elements including conjugative transposons (CTns) and mobilizable transposons (MTns). Tn4555 is an MTn that encodes beta-lactam resistance, and it is efficiently mobilized by the Bacteroides CTns via a tetracycline (TET)-inducible mechanism. In this study a model system with CTn341 and a Tn4555 minielement was used to examine Tn4555 excision from the chromosome. Using PCR and mobilization assays it was established that excision was stimulated by TET in the presence of CTn341. In order to determine which Tn4555 genes were required for excision, int, tnpA, tnpC, xis, and mobA mutants were examined. The results indicated that int plus two additional genes, tnpC and xis, were required for optimal excision. In addition, there was no requirement for the mobA gene, as had been shown for another MTn, NBU1. The Xis protein sequence is related to a family of plasmid excisionases, but the TnpC gene product did not match anything in the sequence databases. Evidence also was obtained that suggested that Xis is involved in the control of TET-induced excision and in control of mobilization by CTn341. Overall, these results indicate that excision of MTns is a complex process that requires multiple gene products. PMID:14702313

Parker, Anita C; Smith, C Jeffrey

2004-01-01

168

Characterization of Tn917 insertion mutants of Staphylococcus epidermidis affected in biofilm formation.  

PubMed Central

Biofilm formation is thought to result from the concerted action of primary attachment to a specific surface and accumulation in multilayered cell clusters. Here we describe the isolation and characterization of transposon (Tn917) mutants of Staphylococcus epidermidis O-47 which were biofilm negative in the polystyrene microtiter plate assay. Among 5,000 Tn917 insertion mutants, 4 biofilm-negative mutants were isolated. Each mutant carried one copy of Tn917. The mutants were divided into two phenotypic classes: class A (mut1 and mut1a) and class B (mut2 and mut2a). Mutants of phenotypic class A lacked four cell surface proteins, were less hydrophobic, and were affected in primary attachment to polystyrene, but were still able to form multilayered cell clusters. They were able to form a biofilm on a glass surface, a trait that was even more pronounced than in the wild-type stain O-47. Loss of several surface proteins might have led to the reduced surface hydrophilic structures, thus favoring primary attachment to a glass surface and leading to subsequent biofilm formation. Mutants of phenotype class B were able to attach to polystyrene but were unable to form multilayered cell clusters, had unchanged cell surface proteins and hydrophobicity, and were unable to form a biofilm on a glass surface, mut1 and mut2 could be complemented by wild-type DNA fragments containing the Tn917 insertion sites of mut1 and mut2, respectively. The complemented biofilm-positive clone mut1 (pRC20) produced a 60-kDa protein which is postulated to function as the adhesin for binding to plastic. The traits of binding to polystyrene and the ability to form multilayered cell clusters are phenotypically and genetically distinct. PMID:8557351

Heilmann, C; Gerke, C; Perdreau-Remington, F; Götz, F

1996-01-01

169

77 FR 59573 - Proposed Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Cities, TN/VA. The Class E surface area airspace designated as an extension...Class E Airspace Designated as Surface Areas * * * * * ASO TN E2 Tri-Cities...Designated as an Extension to a Class D Surface Area. * * * * * ASO TN E4...

2012-09-28

170

UNH Cooperative Extension Educators, Forest Resources County Offices Belknap County  

E-print Network

.nute@unh.edu 603-641-6060 FAX: 603-645-5252 Hillsborough County Mary Tebo Davis Community Forestry Field Specialist 329 Mast Rd., Suite 101 Goffstown, NH 03045 mary.tebo@unh.edu 641-6060 FAX: 645-5252 Merrimack County

New Hampshire, University of

171

Taiwan Nantou County earthquake 0327 Taiwan Nantou County earthquake  

E-print Network

Taiwan Nantou County earthquake 20130327 1 #12;0327 Taiwan Nantou County earthquake Source, Intensity 5 #12;I II III IV V VI VII Intensity Shake map of the March 27 Earthquake The peak ground and Technology Center for Disaster Reduction (NCDR) #12;Earthquake Response and Evacuation are a Part of Students

172

Iron oxide superparamagnetic nanoparticles conjugated with a conformationally blocked ?-Tn antigen mimetic for macrophage activation  

NASA Astrophysics Data System (ADS)

Among new therapies to fight tumors, immunotherapy is still one of the most promising and intriguing. Thanks to the ongoing structural elucidation of several tumor antigens and the development of innovative antigen carriers, immunotherapy is in constant evolution and it is largely used either alone or in synergy with chemotherapy/radiotherapy. With the aim to develop fully synthetic immunostimulants we have recently developed a mimetic of the ?-Tn mucin antigen, a relevant tumor antigen. The 4C1 blocked mimetic 1, unique example of an ?-Tn mimetic antigen, was functionalized with an ?-phosphonate linker and used to decorate iron oxide superparamagnetic nanoparticles (MNPs), employed as multivalent carriers. MNPs, largely exploited for supporting and carrying biomolecules, like antibodies, drugs or antigens, consent to combine in the same nanometric system the main features of an inorganic magnetic core with a bioactive organic coating. The superparamagnetic glyconanoparticles obtained, named GMNPs, are indeed biocompatible and immunoactive, and they preserve suitable characteristics for use as heat mediators in the magnetic fluid hyperthermia (MFH) treatment of tumors. All together these properties make GMNPs attracting devices for innovative tumor treatment.Among new therapies to fight tumors, immunotherapy is still one of the most promising and intriguing. Thanks to the ongoing structural elucidation of several tumor antigens and the development of innovative antigen carriers, immunotherapy is in constant evolution and it is largely used either alone or in synergy with chemotherapy/radiotherapy. With the aim to develop fully synthetic immunostimulants we have recently developed a mimetic of the ?-Tn mucin antigen, a relevant tumor antigen. The 4C1 blocked mimetic 1, unique example of an ?-Tn mimetic antigen, was functionalized with an ?-phosphonate linker and used to decorate iron oxide superparamagnetic nanoparticles (MNPs), employed as multivalent carriers. MNPs, largely exploited for supporting and carrying biomolecules, like antibodies, drugs or antigens, consent to combine in the same nanometric system the main features of an inorganic magnetic core with a bioactive organic coating. The superparamagnetic glyconanoparticles obtained, named GMNPs, are indeed biocompatible and immunoactive, and they preserve suitable characteristics for use as heat mediators in the magnetic fluid hyperthermia (MFH) treatment of tumors. All together these properties make GMNPs attracting devices for innovative tumor treatment. Electronic supplementary information (ESI) available: Optical rotation and spectroscopic data for all compounds; selected TEM images of MNPs, CMNPs and GMNPs; XRD patterns and FTIR spectra of MNPs coated with surfactants and with the ?-Tn mimetic; MNP magnetic properties. See DOI: 10.1039/c4nr00070f

Manuelli, Massimo; Fallarini, Silvia; Lombardi, Grazia; Sangregorio, Claudio; Nativi, Cristina; Richichi, Barbara

2014-06-01

173

Carbon County Secondary Data Analysis  

E-print Network

Carbon County Secondary Data Analysis July 23, 2012 1 1 Community Health Data, MT Dept, Wheatland, Golden Valley, Musselshell, Sweet Grass, Stillwater, Yellowstone, Big Horn, and Carbon. CLRD* #12; Carbon County Secondary Data Analysis July 23, 2012 2 Socioeconomic Measures1

Maxwell, Bruce D.

174

Frederick County Community Perception Survey.  

ERIC Educational Resources Information Center

In 1997, Frederick Community College (FCC) in Maryland conducted a telephone survey of a random sample of 466 Frederick County residents to identify their perceptions of the college. In particular, the survey examined Frederick County residents' image of FCC, level of awareness of services and programs offered by FCC, and the types of services…

Frederick Community Coll., MD.

175

Ravalli County Secondary Data Analysis  

E-print Network

,914 Unemployment Rate 7 8.1% 6.3% 7.7% Persons Below Poverty Level 1 14.0% 14.0% 13.8% Uninsured Adults (Age (%) Education Level County Montana 10 Indicators Northwest, Imp. Graph (2011) #12;Ravalli County Secondary Data.7 Not relevant Age 1 Gender 1 Male Female

Maxwell, Bruce D.

176

Lincoln County Secondary Data Analysis  

E-print Network

,000 $51,914 Unemployment Rate7 14.5% 6.3% 7.7% Persons Below Poverty Level1 19.0% 14.0% 13 (%) Education Level County Montana 10 Indicators Northwest, Imp. Graph (2011) #12; Lincoln County Secondary+ Gender1 Male Female Male Female Male Female

Maxwell, Bruce D.

177

Lake County Secondary Data Analysis  

E-print Network

,914 Unemployment Rate 7 8.9% 6.3% 7.7% Persons Below Poverty Level 1 18.0% 14.0% 13.8% Uninsured Adults (Age (%) Education Level County Montana 10 Indicators Northwest, Imp. Graph (2011) #12;Lake County Secondary Data-64 65+ Gender 1 Male Female Male Female Male Female 49

Maxwell, Bruce D.

178

76 FR 58241 - Opportunity for Designation in the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Administration Opportunity for Designation in the Jamestown, ND; Lincoln, NE; Memphis, TN...agencies: Grain Inspection, Inc. (Jamestown); Lincoln Inspection Service, Inc...the Act. Areas Open for Designation Jamestown Pursuant to Section 7(f)(2)...

2011-09-20

179

77 FR 13073 - Designation for the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA Areas  

Federal Register 2010, 2011, 2012, 2013, 2014

...Stockyards Administration Designation for the Jamestown, ND; Lincoln, NE; Memphis, TN...designation of Grain Inspection, Inc. (Jamestown); Lincoln Inspection Service, Inc...geographic areas presently serviced by Jamestown, Lincoln, Midsouth, and Sioux...

2012-03-05

180

78 FR 2431 - Notice of Inventory Completion: U.S. Department of Energy Oak Ridge Office, Oak Ridge, TN...  

Federal Register 2010, 2011, 2012, 2013, 2014

...of Inventory Completion: U.S. Department of Energy Oak Ridge Office, Oak Ridge, TN; Correction AGENCY: National Park Service...SUMMARY: The U.S. Department of Energy Oak Ridge Office has corrected an inventory of human...

2013-01-11

181

77 FR 68818 - Notice of Inventory Completion: U.S. Department of Energy Oak Ridge Office, Oak Ridge, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...of Inventory Completion: U.S. Department of Energy Oak Ridge Office, Oak Ridge, TN AGENCY: National Park Service, Interior...SUMMARY: The U.S. Department of Energy Oak Ridge Office has completed an inventory of human...

2012-11-16

182

77 FR 66480 - Final Environmental Impact Statement, Narrows Project, Sanpete County, Utah  

Federal Register 2010, 2011, 2012, 2013, 2014

...400 acre-feet of water annually from Gooseberry Creek drainage in the Colorado River...the Sevier River drainage basin. The water right used under...an average annual supply of 4,281 acre-feet... Main Interior Building, Natural...

2012-11-05

183

The Listeria monocytogenes transposon Tn6188 provides increased tolerance to various quaternary ammonium compounds and ethidium bromide.  

PubMed

Tolerance of the foodborne pathogen Listeria monocytogenes to sublethal concentrations of disinfectants has been frequently reported. Particularly, quaternary ammonium compounds (QACs) such as benzalkonium chloride (BC) are often used in disinfectants and also as antiseptics in food industry and hospitals. Recently, we described Tn6188, a novel transposon in L. monocytogenes harbouring the transporter QacH, a molecular mechanism leading to increased tolerance to BC. In this study, we investigated the presence of Tn6188 within the genus Listeria spp. Our screening indicates that the distribution of Tn6188 may be limited to L. monocytogenes. We confirm that QacH is responsible for the observed increase in tolerance by complementation of a qacH deletion mutant and introducing qacH in a Tn6188 negative strain. We investigated the transporter's substrate spectrum by determining minimal inhibitory concentrations (MICs) and showed that QacH also confers higher tolerance towards other QACs and ethidium bromide (EtBr). This result was supported by increased expression of qacH in the presence of the various substrates as determined by quantitative reverse transcriptase PCR (qRT-PCR). In addition, we detected expression of a Tn6188 transposase gene and circular forms of Tn6188, suggesting activity and possible transfer of this transposon. PMID:25312720

Müller, Anneliese; Rychli, Kathrin; Zaiser, Andreas; Wieser, Cornelia; Wagner, Martin; Schmitz-Esser, Stephan

2014-12-01

184

Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA.  

PubMed Central

The bacterial transposon Tn7 encodes five transposition genes tnsABCDE. We report a simple and rapid procedure for the purification of TnsC protein. We show that purified TnsC is active in and required for Tn7 transposition in a cell-free recombination system. This finding demonstrates that TnsC participates directly in Tn7 transposition and explains the requirement for tnsC function in Tn7 transposition. We have found that TnsC binds adenine nucleotides and is thus a likely site of action of the essential ATP cofactor in Tn7 transposition. We also report that TnsC binds non-specifically to DNA in the presence of ATP or the generally non-hydrolyzable analogues AMP-PNP and ATP-gamma-S, and that TnsC displays little affinity for DNA in the presence of ADP. We speculate that TnsC plays a central role in the selection of target DNA during Tn7 transposition. Images PMID:1317955

Gamas, P; Craig, N L

1992-01-01

185

Metabolite Profiling Reveals Abiotic Stress Tolerance in Tn5 Mutant of Pseudomonas putida.  

PubMed

Pseudomonas is an efficient plant growth-promoting rhizobacteria (PGPR); however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5) mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC) and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE) and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS) pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA) on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic stress tolerant metabolites from the NBRI1108T suggest that Tn5 mutagenesis enhanced tolerance towards high temperature and drought. Tolerance to drought was further confirmed in greenhouse experiments with maize as host plant, where NBRI1108T showed relatively high biomass under drought conditions. PMID:25629312

Chaudhry, Vasvi; Bhatia, Anil; Bharti, Santosh Kumar; Mishra, Shashank Kumar; Chauhan, Puneet Singh; Mishra, Aradhana; Sidhu, Om Prakash; Nautiyal, Chandra Shekhar

2015-01-01

186

Characterization of a transposon Tn916-generated mutant of Haemophilus ducreyi 35000 defective in lipooligosaccharide biosynthesis.  

PubMed Central

To define the role of the surface lipooligosaccharide (LOS) of Haemophilus ducreyi in the pathogenesis of chancroid, Tn916 mutants of H. ducreyi 35000 defective in expression of the murine monoclonal antibody (MAb) 3F11 epitope on H. ducreyi LOS were identified by immunologic screening. One mutant, designated 1381, has an LOS which lacks the MAb 3F11 epitope and migrates with an increased mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The gene disrupted by the Tn916 element in strain 1381 was identified by cloning the sequences flanking the Tn916 element. The sequences were then used to probe a lambda DASHII genomic library. In strain 1381, Tn916 interrupts a gene which encodes an open reading frame (ORF) with an Mr of 40,246. This ORF has homology to the product of the rfaK gene of Escherichia coli. The major LOS glycoform produced by strain 1381 was analyzed by using a combination of mass spectrometry, linkage and composition analysis, and 1H nuclear magnetic resonance spectroscopy. The major LOS species was found to terminate in a single glucose attached to the heptose (L-glycero-D-manno-heptose, or Hep) trisaccharide core. In the wild-type strain 35000, glucose serves as the acceptor for the addition of the D-glycero-D-manno-heptose (or DDHep), which extends to form the mature branch of the H. ducreyi LOS. This mature oligosaccharide is in turn partially capped by the addition of sialic acid (NeuAc), i.e., NeuAc2 alpha-->3Gal beta1-->4GlcNAc beta1-->3Gal beta1-->4DDHep alpha1-->6Glc beta1 (W. Melaugh et al., Biochemistry 33:13070-13078, 1994). Since this LOS terminates prior to the addition of the branch DD-heptose, this gene is likely to encode the D-glycero-D-manno-heptosyltransferase. Strain 1381 exhibits a significant reduction in adherence to and invasion of primary human keratinocytes. This defect was complemented by the cloned heptosyltransferase gene, indicating that the terminal portion of the LOS oligosaccharide plays an important role in adherence to human keratinocytes. PMID:9260947

Gibson, B W; Campagnari, A A; Melaugh, W; Phillips, N J; Apicella, M A; Grass, S; Wang, J; Palmer, K L; Munson, R S

1997-01-01

187

Metabolite Profiling Reveals Abiotic Stress Tolerance in Tn5 Mutant of Pseudomonas putida  

PubMed Central

Pseudomonas is an efficient plant growth–promoting rhizobacteria (PGPR); however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5) mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC) and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE) and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS) pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA) on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic stress tolerant metabolites from the NBRI1108T suggest that Tn5 mutagenesis enhanced tolerance towards high temperature and drought. Tolerance to drought was further confirmed in greenhouse experiments with maize as host plant, where NBRI1108T showed relatively high biomass under drought conditions. PMID:25629312

Chaudhry, Vasvi; Bhatia, Anil; Bharti, Santosh Kumar; Mishra, Shashank Kumar; Chauhan, Puneet Singh; Mishra, Aradhana; Sidhu, Om Prakash; Nautiyal, Chandra Shekhar

2015-01-01

188

Tennessee Higher Education County Profiles, 2008  

ERIC Educational Resources Information Center

This report presents a localized perspective on Tennessee higher education, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; (3) number of county residents enrolled in Tennessee public institutions; and (4) number of county residents participating in the…

Tennessee Higher Education Commission, 2008

2008-01-01

189

The Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD  

PubMed Central

The excision of transposon Tn7 from a donor site and its insertion into its preferred target site, attachment site attTn7, is mediated by four Tn7-encoded transposition proteins: TnsA, TnsB, TnsC, and TnsD. Transposition requires the assembly of a nucleoprotein complex containing all four Tns proteins and the DNA substrates, the donor site containing Tn7, and the preferred target site attTn7. TnsA and TnsB together form the heteromeric Tn7 transposase, and TnsD is a target-selecting protein that binds specifically to attTn7. TnsC is the key regulator of transposition, interacting with both the TnsAB transposase and TnsD-attTn7. We show here that TnsC interacts directly with TnsB, and identify the specific region of TnsC involved in the TnsB–TnsC interaction during transposition. We also show that a TnsC mutant defective in interaction with TnsB is defective for Tn7 transposition both in vitro and in vivo. Tn7 displays cis-acting target immunity, which blocks Tn7 insertion into a target DNA that already contains Tn7. We provide evidence that the direct TnsB–TnsC interaction that we have identified also mediates cis-acting Tn7 target immunity. We also show that TnsC interacts directly with the target selector protein TnsD. PMID:24982178

Choi, Ki Young; Spencer, Jeanelle M.; Craig, Nancy L.

2014-01-01

190

Fault Trace: Marin County, California  

NSDL National Science Digital Library

This photograph shows the trace of a fault (in trench phase) as it passes beneath a barn. The trace developed during the April 18, 1906 San Francisco Earthquake. The location is the Skinner Ranch, near Olema, Marin County, California.

191

Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha  

SciTech Connect

Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the inability to grow with reduced sulfur compounds (Sox/sup -/). Four classes of Sox/sup -/ mutants were obtained. Three were of different pleiotropic phenotypes: (i) unable to grow with formate, nitrate, and xanthine; (this class strongly suggested the involvement of a molybdenum cofactor in inorganic sulfur-oxidizing ability); (ii) no growth with hydrogen; (iii) slight growth with hydrogen and formate. Two plasmids, pHG41 (about 450 kilobase pairs) and pHG42 (110 kilobases), were identified in lysates of T. pantotropha. In one Sox/sup -/ mutant pHG41 could not be detected. Revertant analysis suggested that pHG41 and pHG42 were not involved in the Sox character.

Chandra, T.S.; Friedrich, C.G.

1986-05-01

192

The Sugar Creek zinc deposit, Jackson Co. TN -- Exploration history, geology and mineralization  

SciTech Connect

During the 60's and 70's zinc exploration of central TN and KY was active. The Sugar Creek Project was one of several investigated by Exxon. The discovery hole, Cu 15, was drilled in early 1973. The Sugar Creek Zinc Deposit was acquired by Independence Mining Co. in 1986 and I.M.C. has subsequently completed additional drilling, both stepout and confirmation holes. A total of 137 holes for 300,833 ft have been drilled. The Sugar Creek deposit is a typical Tennessee zinc deposit (Mississippi Valley Type) which occurs in solution collapse breccias in the Lower Ordovician, Knox Dolomite. The Knox consists of fine grained dolomite with interlayered limestones and crystalline dolomite. Only scattered residual limestone is found in the Sugar Creek area. Collapse breccias have formed which control zinc deposition and are similar to other TN Zn. deposits. At Sugar Creek the types of breccias include: a vertically exaggerated glory hole breakthrough breccia which extends to within 137 ft. of the Knox unconformity, has 500 ft. of zinc mineralization with 8 significant zinc intervals; holes with stacked zinc intervals interpreted to be sides of breakthrough breccia; and single zinc intervals in laterally positioned bedded mineral zones. A total of 99 holes were drilled in the more intense mineralized areas. The ratio of ore to non ore holes is nearly 1 to 1. The mineralization is typical M.V.T. with predominantly sphalerite and only minor occurrences of galena, fluorite, pyrite, etc.

Reinbold, G.; Moran, A.V.; Stevens, D.L. (Independence Mining Co. Inc., Reno, NV (United States))

1993-03-01

193

Bioactive secondary metabolites from a new terrestrial Streptomyces sp. TN262.  

PubMed

During our search for Streptomyces spp. as new producers of bioactive secondary metabolites, the ethyl acetate extract of the new terrestrial Streptomyces isolate TN262 delivered eight antimicrobially active compounds. They were identified as 1-acetyl-beta-carboline (1), tryptophol (2), cineromycin B (3), 2,3-dihydrocineromycin B (4), cyclo-(tyrosylprolyl) (5), 3-(hydroxyacetyl)-indole (6), brevianamide F (7), and cis-cyclo-(L-prolyl-L-leucyl) (8). Three further metabolites were detected in the unpolar fractions using GC-MS and tentatively assigned as benzophenone (9), N-butyl-benzenesulfonamide (10), and hexanedioic acid-bis-(2-ethylhexyl) ester (11). This last compound is known as plasticizer derivatives, but it has never been described from natural sources. In this article, we describe the identification of the new Streptomyces sp. isolate TN262 using its cultural characteristics, the nucleotide sequence of the corresponding 16S rRNA gene and the phylogenetic analysis, followed by optimization, large-scale fermentation, isolation of the bioactive constituents, and determination of their structures. The biological activity of compounds (2), (3), (4), and those of the unpolar fractions was addressed as well. PMID:19842066

Elleuch, Lobna; Shaaban, Mohamed; Smaoui, Slim; Mellouli, Lotfi; Karray-Rebai, Ines; Fourati-Ben Fguira, Lilia; Shaaban, Khaled A; Laatsch, Hartmut

2010-09-01

194

Geothermal development plan: Yuma county  

SciTech Connect

One hot spring and 33 wells drilled in the county discharge water at temperatures sufficient for direct-use geothermal applications such as process heat and space heating and cooling. Currently, one industry within the county has been identified which may be able to use geothermal energy for its process heat requirements. Also, a computer simulation model was used to predict geothermal energy on line as a function of time under both private and city-owned utility development of the resource.

White, D.H.

1981-01-01

195

Cardiac troponin I but not cardiac troponin T adheres to polysulfone dialyser membranes in an in vitro haemodialysis model: explanation for lower serum cTnI concentrations following dialysis  

PubMed Central

Background Elevated serum cardiac troponin T (cTnT) and I (cTnI) can occur in patients with chronic kidney disease. Differences in cTn concentrations between cTnT and cTnI have been reported but the mechanism of such discrepancy has not been investigated. This study investigates the clearance of cTn with the aid of an in vitro model of haemodialysis (HD). Methods Serum was obtained before and after a single session of dialysis from 53 patients receiving HD and assayed for cTnT and cTnI. An in vitro model of the dialysis process was used to investigate the mechanism of clearance of cTn during HD. Results Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD. Using an in vitro model of dialysis, it was demonstrated that cTnI is not dialysed from the vascular compartment but adheres to the dialyser membrane. Conclusions The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis. The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar. PMID:25332816

Gaze, David C; Collinson, Paul O

2014-01-01

196

Identification of a novel composite transposable element, Tn5280, carrying chlorobenzene dioxygenase genes of Pseudomonas sp. strain P51.  

PubMed Central

Analysis of one of the regions of catabolic plasmid pP51 which encode chlorobenzene metabolism of Pseudomonas sp. strain P51 revealed that the tcbA and tcbB genes for chlorobenzene dioxygenase and dehydrogenase are located on a transposable element, Tn5280. Tn5280 showed the features of a composite bacterial transposon with iso-insertion elements (IS1066 and IS1067) at each end of the transposon oriented in an inverted position. When a 12-kb HindIII fragment of pP51 containing Tn5280 was cloned in the suicide donor plasmid pSUP202, marked with a kanamycin resistance gene, and introduced into Pseudomonas putida donor plasmid pSUP202, marked with a kanamycin resistance gene, and introduced into Pseudomonas putida KT2442, Tn5280 was found to transpose into the genome at random and in single copy. The insertion elements IS1066 and IS1067 differed in a single base apir located in the inner inverted repeat and were found to be highly homologous to a class of repetitive elements of Bradyrhizobium japonicum and distantly related to IS630 of Shigella sonnei. The presence of the catabolic genes tcbA and tcbB on Tn5280 suggests a mechanism by which gene clusters can be mobilized as gene cassettes and joined with others to form novel catabolic pathways. Images FIG. 3 FIG. 4 PMID:1657878

van der Meer, J R; Zehnder, A J; de Vos, W M

1991-01-01

197

Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains  

PubMed Central

Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch. PMID:19547659

Sioud, Samiha; Aigle, Bertrand; Karray-Rebai, Ines; Smaoui, Slim; Bejar, Samir; Mellouli, Lotfi

2009-01-01

198

TnI-Ultra assay measurements in cancer patients: comparison with the conventional assay and clinical implication.  

PubMed

The serial monitoring of cardiac troponin represents an effective approach for the early identification, assessment, and monitoring of chemotherapy-induced cardiac injury. Over the last few years new generations of troponin assays, referred to as sensitive and high sensitivity assays, able to detect very low concentrations of troponin, have been progressively released on different platforms. Some studies have assessed the comparability of the cTnI measurements with the new assays versus the conventional ones, but none of these in the oncological population. We compared the cTnI results determined on Stratus CS and ADVIA Centaur CP System in 70 breast cancer patients, for a total of 327 samples collected during different cycles of treatment. Correlation (Spearman = 0.732) and agreement (91.4%) between the assays were good (244 concordant negatives and 55 concordant positives), with a frequency of 8.6% discordant results among the cTnI measurements. Despite the well-known lack in the harmonization and standardization of the currently commercially available cTnI methods, we found a good clinical concordance of cTnI determination on both systems. PMID:24693994

Salvatici, Michela; Cardinale, Daniela; Botteri, Edoardo; Bagnardi, Vincenzo; Mauro, Cristian; Cassatella, Maria C; Lentati, Paola; Bottari, Fabio; Zorzino, Laura; Passerini, Rita; Cipolla, Carlo M; Sandri, Maria T

2014-08-01

199

Fast electro-optic switching of frequency modulation TN-LCDs fabricated by doping nanoparticles and their mechanism  

NASA Astrophysics Data System (ADS)

Electro-optic characteristics of a twisted nematic (TN) liquid crystal display (LCD) fabricated by doping Ag nanoparticles protected with NLC, 5CB (K-15, Merck) molecules has been investigated and it is shown that the device exhibits a unique electrooptic response characteristics that is sensitive to the high frequency component of the operating voltage together with the conventional root-mean-square response to the operating voltage. We call this device frequency modulation (FM) TN-LCD. The FM TN-LCD is switched by switching the frequency of the operating voltage while its amplitude is kept unchanged. As an example, by switching the frequency from 20Hz to 500Hz or 50Hz to 2kHz, and the switching is done with the time constant of several ms. This device shows a peculiar response to a burst AC square voltage waveforms, where the device exhibits bipolar response with the time constant of 500 ms and the envelope with the time constants shorter than those of an undoped TN-LCD by from 30% to 80%. The mechanism of the FM-TN-LCD is investigated through the study of its dielectric properties.

Miyama, Tomohiro; Kundu, Sudarshan; Shiraki, Hiroyuki; Sakai, Yoshio; Shiraishi, Yukihide; Toshima, Naoki; Kobayashi, Shunsuke

2004-05-01

200

San Bernardino County Museum  

NSDL National Science Digital Library

The San Bernardino County Museum is a regional museum with exhibits and collections in cultural and natural history. Special exhibits, the Exploration Station live animal discovery center, extensive research collections, and public programs for adults, families, students, and children are all part of the museum experience. The Museum is surrounded by citrus groves, and orange blossoms perfume the air. The Zimmerman Citrus Kiosk explores citrus agricultural history in Southern California. Death Valley Alive! is a new traveling exhibit, available for rent, where visitors can explore the natural history and culture of the Death Valley region from its geologic beginnings 1.3 billion years ago. Online exhibits include The Importance of Museum Collections, The Barstow Fossil Beds, Motherlode of the Miocene, The Etiwanda Fan, and for kids, Mimicry, a study in camouflage and adaptation. Teacher Resources include professional development and workshops, and Trading Places, where 21 hours of time spent volunteering in the Museum earns a free program for your class. Sample opportunities include writing activities for study kits, developing post-visit activities, creating a museum gallery guide, translating student materials into Spanish, or developing an independent idea. There are programs for youth, Scouts, and adults, as well as various publications available in anthropology, archeology, biology, geology and history.

201

75 FR 25308 - Environmental Impact Statement: Winnebago County, IL and Rock County, WI  

Federal Register 2010, 2011, 2012, 2013, 2014

...Administration Environmental Impact Statement: Winnebago County, IL and Rock County, WI AGENCY: Federal Highway Administration (FHWA...Wisconsin Route 213 and Nye School Road northwest of Beloit, Rock County, Wisconsin to the interchange of Rockton Road and...

2010-05-07

202

75 FR 52367 - Notice of Inventory Completion: Memphis Pink Palace Museum, Memphis, TN  

Federal Register 2010, 2011, 2012, 2013, 2014

...and Tipton counties as the `Big Hatchie Country' and I think that `mound' just got added on. Certainly the bulk of his stuff is from Morgan's Point/Bishop (40TP10).'' The human remains were loaned to the museum in 1939, and the loan was...

2010-08-25

203

Tn5384, a composite enterococcal mobile element conferring resistance to erythromycin and gentamicin whose ends are directly repeated copies of IS256.  

PubMed Central

We have identified a 26-kb mobile element from Enterococcus faecalis CH116, designated Tn5384, which confers resistance to erythromycin and to high levels of gentamicin. Tn5384 is a composite element containing three copies of insertion element IS256. Two of the IS256 copies flank the aac6'-aph2" bifunctional aminoglycoside-modifying-enzyme gene in the inverted orientation, forming a structure similar to staphylococcal gentamicin resistance transposon Tn4001. One of the IS256 elements involved in the Tn4001-like structure also forms the left end of Tn5384, the right end of which is a directly repeated insertion of IS256 approximately 23 kb downstream of the leftmost insertion. Insertions of Tn5384 into enterococcal plasmid pLRM1 have been found associated with 8- and 9-bp duplications of the target sequence. PMID:7625803

Rice, L B; Carias, L L; Marshall, S H

1995-01-01

204

Glycan elongation beyond the mucin associated Tn antigen protects tumor cells from immune-mediated killing.  

PubMed

Membrane bound mucins are up-regulated and aberrantly glycosylated during malignant transformation in many cancer cells. This results in a negatively charged glycoprotein coat which may protect cancer cells from immune surveillance. However, only limited data have so far demonstrated the critical steps in glycan elongation that make aberrantly glycosylated mucins affect the interaction between cancer cells and cytotoxic effector cells of the immune system. Tn (GalNAc-Ser/Thr), STn (NeuAc?2-6GalNAc-Ser/Thr), T (Gal?1-3GalNAc-Ser/Thr), and ST (NeuAc?2-6Gal?1-3GalNAc-Ser/Thr) antigens are recognized as cancer associated truncated glycans, and are expressed in many adenocarcinomas, e.g. breast- and pancreatic cancer cells. To investigate the role of the cancer associated glycan truncations in immune-mediated killing we created glyco-engineered breast- and pancreatic cancer cells expressing only the shortest possible mucin-like glycans (Tn and STn). Glyco-engineering was performed by zinc finger nuclease (ZFN) knockout (KO) of the Core 1 enzyme chaperone COSMC, thereby preventing glycan elongation beyond the initial GalNAc residue in O-linked glycans. We find that COSMC KO in the breast and pancreatic cancer cell lines T47D and Capan-1 increases sensitivity to both NK cell mediated antibody-dependent cellular-cytotoxicity (ADCC) and cytotoxic T lymphocyte (CTL)-mediated killing. In addition, we investigated the association between total cell surface expression of MUC1/MUC16 and NK or CTL mediated killing, and observed an inverse correlation between MUC16/MUC1 expression and the sensitivity to ADCC and CTL-mediated killing. Together, these data suggest that up-regulation of membrane bound mucins protects cells from immune mediated killing, and that particular glycosylation steps, as demonstrated for glycan elongation beyond Tn and STn, can be important for fine tuning of the immune escape mechanisms in cancer cells. PMID:24039759

Madsen, Caroline B; Lavrsen, Kirstine; Steentoft, Catharina; Vester-Christensen, Malene B; Clausen, Henrik; Wandall, Hans H; Pedersen, Anders Elm

2013-01-01

205

Glycan Elongation Beyond the Mucin Associated Tn Antigen Protects Tumor Cells from Immune-Mediated Killing  

PubMed Central

Membrane bound mucins are up-regulated and aberrantly glycosylated during malignant transformation in many cancer cells. This results in a negatively charged glycoprotein coat which may protect cancer cells from immune surveillance. However, only limited data have so far demonstrated the critical steps in glycan elongation that make aberrantly glycosylated mucins affect the interaction between cancer cells and cytotoxic effector cells of the immune system. Tn (GalNAc-Ser/Thr), STn (NeuAc?2-6GalNAc-Ser/Thr), T (Gal?1–3GalNAc-Ser/Thr), and ST (NeuAc?2-6Gal?1–3GalNAc-Ser/Thr) antigens are recognized as cancer associated truncated glycans, and are expressed in many adenocarcinomas, e.g. breast- and pancreatic cancer cells. To investigate the role of the cancer associated glycan truncations in immune-mediated killing we created glyco-engineered breast- and pancreatic cancer cells expressing only the shortest possible mucin-like glycans (Tn and STn). Glyco-engineering was performed by zinc finger nuclease (ZFN) knockout (KO) of the Core 1 enzyme chaperone COSMC, thereby preventing glycan elongation beyond the initial GalNAc residue in O-linked glycans. We find that COSMC KO in the breast and pancreatic cancer cell lines T47D and Capan-1 increases sensitivity to both NK cell mediated antibody-dependent cellular-cytotoxicity (ADCC) and cytotoxic T lymphocyte (CTL)-mediated killing. In addition, we investigated the association between total cell surface expression of MUC1/MUC16 and NK or CTL mediated killing, and observed an inverse correlation between MUC16/MUC1 expression and the sensitivity to ADCC and CTL-mediated killing. Together, these data suggest that up-regulation of membrane bound mucins protects cells from immune mediated killing, and that particular glycosylation steps, as demonstrated for glycan elongation beyond Tn and STn, can be important for fine tuning of the immune escape mechanisms in cancer cells. PMID:24039759

Madsen, Caroline B.; Lavrsen, Kirstine; Steentoft, Catharina; Vester-Christensen, Malene B.; Clausen, Henrik

2013-01-01

206

One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI.  

PubMed

2-Aminobenzyl amine (2-ABA) functionalized graphene is proposed for the ultrasensitive immunosensing of Cardiac Troponin I (cTnI). 2-ABA was electrochemically polymerized on the graphene decorated interdigitated electrode to obtain the amine functionalized graphene (f-GN). The f-GN electrode was then modified with monoclonal anti-cTnI antibodies via Schiff reaction based chemistry. Detailed characteristics of the processes involved and the finally developed antibody conjugated f-GN interdigitated electrode have been studied. The above micro-device was used in a drain source configuration for the sensing of cTnI. A wide dynamic linear range of antigen detection (0.01-1ng/mL) is achieved with the limit of detection of 0.01ng/mL. The utility of the proposed sensing technique is demonstrated by successfully testing the antigen concentration in spiked serum samples. PMID:25460893

Tuteja, Satish K; Kukkar, Manil; Suri, C R; Paul, A K; Deep, Akash

2015-04-15

207

Differential analysis of transient increases of serum cTnI in response to handling in rats.  

PubMed

Serum cardiac troponins are the key biomarkers of myocardial necrosis in humans and in preclinical species. The use of ultrasensitive assays for serum cardiac troponin I (cTnI) as a biomarker in safety studies is hampered by interindividual differences. In this study, we investigated the effect of handling procedures on serum cTnI and explored modeling and simulation approaches to mitigate the impact of these interindividual differences. Femoral-catheterized male Crl:WI(Han) rats (n = 16/group) were left undisturbed in their cages with no handling; subjected to 5 min of isoflurane/O2 anesthesia (A); or placed into a rodent restrainer followed by simulated tail vein injection (RR). Serum cTnI concentrations were assessed over a 24-h period using an ultrasensitive assay, and the study was repeated for confirmation. The mean serum cTnI concentration pre-procedure was 4.2 pg/mL, and remained stable throughout the duration of the study in the rats submitted to the A procedure. Serum cTnI concentrations increased transiently after the RR procedure with a median time to maximum concentration (T max), of 1 and 2 h and a mean maximum value concentration (C max), of 53.0 and 7.2 pg/mL in the initial and repeat studies, respectively. A population pharmacodynamic model identified interindividual, procedure- and study-specific effects on serum cTnI concentrations in rats. It is concluded that a modeling and simulation approach more appropriately describes and statistically analyzes the data obtained with this ultrasensitive assays. PMID:25505566

Mikaelian, Igor; Dunn, Michael E; Mould, Diane R; Hirkaler, Gerard; Geng, Wanping; Coluccio, Denise; Nicklaus, Rosemary; Singer, Thomas; Reddy, Micaela

2013-12-01

208

Tn Glycosylation of the MUC6 Protein Modulates Its Immunogenicity and Promotes the Induction of Th17-biased T Cell Responses*  

PubMed Central

The Tn antigen (?-GalNAc-O-Ser/Thr) is one of the most specific human cancer-associated structures. This antigen, together with mucins, the major carriers of O-glycosylated tumor antigens in adenocarcinomas, are being evaluated as anti-cancer immunotherapeutic targets. In particular, the MUC6 protein, which is normally expressed only in gastric tissues, has been detected in intestinal, pulmonary, colorectal, and breast carcinomas. To develop anti-cancer vaccines based on the Tn antigen, we produced MUC6 proteins with different Tn density by using mixtures of recombinant ppGalNAc-T1, -T2, and -T7. The obtained glycoproteins were characterized and analyzed for their immunological properties, as compared with the non-glycosylated MUC6. We show that these various MUC6:Tn glycoproteins were well recognized by both MUC6 and Tn-specific antibodies. However, Tn glycosylation of the MUC6 protein strongly affected their immunogenicity by partially abrogating Th1 cell responses, and promoting IL-17 responses. Moreover, the non-glycosylated MUC6 was more efficiently presented than MUC6:Tn glycoproteins to specific T CD4+ hybridomas, suggesting that Tn glycosylation may affect MUC6 processing or MHC binding of the processed peptides. In conclusion, our results indicate that Tn glycosylation of the MUC6 protein strongly affects its B and T cell immunogenicity, and might favor immune escape of tumor cells. PMID:21193402

Freire, Teresa; Lo-Man, Richard; Bay, Sylvie; Leclerc, Claude

2011-01-01

209

Population substructure in Cache County, Utah: the Cache County study  

PubMed Central

Background Population stratification is a key concern for genetic association analyses. In addition, extreme homogeneity of ethnic origins of a population can make it difficult to interpret how genetic associations in that population may translate into other populations. Here we have evaluated the genetic substructure of samples from the Cache County study relative to the HapMap Reference populations and data from the Alzheimer's Disease Neuroimaging Initiative (ADNI). Results Our findings show that the Cache County study is similar in ethnic diversity to the self-reported "Whites" in the ADNI sample and less homogenous than the HapMap CEU population. Conclusions We conclude that the Cache County study is genetically representative of the general European American population in the USA and is an appropriate population for conducting broadly applicable genetic studies. PMID:25078123

2014-01-01

210

Interplay of frustration and magnetic field in the two-dimensional quantum antiferromagnet Cu(tn)Cl2  

Microsoft Academic Search

Specific heat and ac magnetic susceptibility measurements, spanning low temperatures (T>=40mK) and high-magnetic fields (B<=14T) , have been performed on a two-dimensional (2D) antiferromagnet Cu(tn)Cl2 (tn=1,3-diaminopropane=C3H10N2) . The compound represents a S=1\\/2 spatially anisotropic triangular antiferromagnet realized by a square lattice with nearest-neighbor (J\\/kB=3K) , frustrating next-nearest-neighbor (0

A. Orendácová; E. Cizmár; L. Sedláková; J. Hanko; M. Kajnaková; M. Orendác; A. Feher; J. S. Xia; L. Yin; D. M. Pajerowski; M. W. Meisel; V. Zelenák; S. Zvyagin; J. Wosnitza

2009-01-01

211

Exact partition functions of Higgsed 5d $T_N$ theories  

E-print Network

We present a general prescription by which we can systematically compute exact partition functions of five-dimensional supersymmetric theories which arise in Higgs branches of the $T_N$ theory. The theories may be realized by webs of 5-branes whose dual geometries are non-toric. We have checked our method by calculating the partition functions of the theories realized in various Higgs branches of the $T_3$ theory. A particularly interesting example is the $E_8$ theory which can be obtained by Higgsing the $T_6$ theory. We explicitly compute the partition function of the $E_8$ theory and find the agreement with the field theory result as well as the enhancement of the global symmetry to $E_8$.

Hirotaka Hayashi; Gianluca Zoccarato

2015-01-19

212

A double diastereoselective Michael-type addition as an entry to conformationally restricted tn antigen mimics.  

PubMed

A totally stereocontrolled C-Michael addition of serine-equivalent C-nucleophiles to tri-O-benzyl-2-nitro-d-galactal was used as the key step to synthesize several pyrano[3,2-b]pyrrole structures. These scaffolds could be regarded as conformationally restricted Tn antigen mimics, as we have demonstrated by biological assays. The pyranose rings retain their (4)C1 chair conformation, as shown by molecular modeling and NMR spectroscopy. The expected bioactivity was established by a competition-tailored enzyme-linked lectin assay using both soybean and Vicia villosa agglutinins as model lectins. The facile described synthetic route and the strategic combination of computational and experimental techniques to reveal conformational features and bioactivity demonstrate the prepared glycomimics to be promising candidates for further exploitation of this scaffold to give glycans for lectin blocking and vaccination. PMID:24083620

Aydillo, Carlos; Navo, Claudio D; Busto, Jesús H; Corzana, Francisco; Zurbano, María M; Avenoza, Alberto; Peregrina, Jesús M

2013-11-01

213

Geothermal development plan: Yuma County  

SciTech Connect

The Yuma County Area Development Plan evaluated the county-wide market potential for utilizing geothermal energy. The study identified four potential geothermal resource areas with temperatures less than 90/sup 0/C (194/sup 0/F), and in addition, two areas are inferred to contain geothermal resources with intermediate (90/sup 0/C to 150/sup 0/C, 194/sup 0/F to 300/sup 0/F) temperature potential. The resource areas are isolated, although one resource area is located near Yuma, Arizona. One resource site is inferred to contain a hot dry rock resource. Anticipated population growth in the county is expected to be 2 percent per year over the next 40 years. The primary employment sector is agriculture, though some light industry is located in the county. Water supplies are found to be adequate to support future growth without advese affect on agriculture. Six firms were found in Yuma County which may be able to utilize geothermal energy for process heat needs. In addition, several agricultural processors were found, concentrated in citrus processing and livestock raising. Geothermal energy utilization projections suggest that by the year 2000, geothermal energy may economically provide the energy equivalent of 53,000 barrels of oil per year to the industrial sector if developed privately. Geothermal utilization projections increase to 132,000 barrels of oil per year by 2000 if a municipal utility developed the resource.

White, D.H.; Goldstone, L.A.

1982-08-01

214

Immunization of breast cancer patients using a synthetic sialyl-Tn glycoconjugate plus Detox adjuvant.  

PubMed

We have synthesized various formulations that have potential for active specific immunotherapy (ASI) of human cancers. Sialyl-Tn (STn) is a potentially important target structure for ASI because its expression on mucins is a strong, independent predictor of poor prognosis, suggesting that it may have functional significance in the metastatic process. In this first pilot study of synthetic sialyl-Tn hapten conjugated to keyhole limpet hemocyanin (STn-KLH), with Detox adjuvant, toxicity and humoral immunogenicity were assessed in 12 patients with metastatic breast cancer. Toxicity was minimal, restricted to local cutaneous reactions (apart from transient nausea and vomiting following single low-dose cyclophosphamide treatment). Using STn-conjugated human serum albumin in a solid-phase enzyme-linked immunosorbent assay, it was shown that all patients developed IgM and IgG specific for the synthetic STn hapten. Following immunization, most patients were shown to develop increased titres of complement-mediated cytotoxic antibodies, partially inhibited by synthetic STn hapten, but not by the related TF hapten. We also detected IgM and IgG antibodies reactive with natural STn determinants expressed on ovine submaxillary mucin, the STn specificity of this reactivity being confirmed by hapten inhibition. Evaluation of clinical efficacy in a small pilot study is difficult. Five patients are alive 12 or more months after entry, and another 4 patients are alive 6 or more months after entry into the study. All 3 patients with known widespread bulky disease progressed despite ASI, 2 having died from widespread cancer. Two patients had partial responses, each lasting 6 months. While several patients had disease stability for 3-10 months, 1 patient with pulmonary metastases remains stable 15 months after entry into the program. PMID:8439984

MacLean, G D; Reddish, M; Koganty, R R; Wong, T; Gandhi, S; Smolenski, M; Samuel, J; Nabholtz, J M; Longenecker, B M

1993-01-01

215

TnBP?Triton X-45 Treatment of Plasma for Transfusion Efficiently Inactivates Hepatitis C Virus  

PubMed Central

Risk of transmission of hepatitis C virus (HCV) by clinical plasma remains high in countries with a high prevalence of hepatitis C, justifying the implementation of viral inactivation treatments. In this study, we assessed the extent of inactivation of HCV during minipool solvent/detergent (SD; 1% TnBP / 1% Triton X-45) treatment of human plasma. Luciferase-tagged infectious cell culture-derived HCV (HCVcc) particles were used to spike human plasma prior to treatment by SD at 31 ± 0.5°C for 30 min. Samples were taken before and after SD treatment and filtered on a Sep-Pak Plus C18 cartridge to remove the SD agents. Risk of cytotoxicity was assessed by XTT cell viability assay. Viral infectivity was analyzed based on the luciferase signals, 50% tissue culture infectious dose viral titer, and immunofluorescence staining for HCV NS5A protein. Total protein, cholesterol, and triglyceride contents were determined before and after SD treatment and C18 cartridge filtration. Binding analysis, using patient-derived HCV clinical isolates, was also examined to validate the efficacy of the inactivation by SD. SD treatment effectively inactivated HCVcc within 30 min, as demonstrated by the baseline level of reporter signals, total loss of viral infectivity, and absence of viral protein NS5A. SD specifically targeted HCV particles to render them inactive, with essentially no effect on plasma protein content and hemostatic function. More importantly, the efficacy of the SD inactivation method was confirmed against various genotypes of patient-derived HCV clinical isolates and against HCVcc infection of primary human hepatocytes. Therefore, treatment by 1% TnBP / 1% Triton X-45 at 31°C is highly efficient to inactivate HCV in plasma for transfusion, showing its capacity to enhance the safety of therapeutic plasma products. We propose that the methodology used here to study HCV infectivity can be valuable in the validation of viral inactivation and removal processes of human plasma-derived products. PMID:25658612

Chou, Ming-Li; Burnouf, Thierry; Chang, Shun-Pang; Hung, Ting-Chun; Lin, Chun-Ching; Richardson, Christopher D.; Lin, Liang-Tzung

2015-01-01

216

Archuleta County CO Lineaments  

SciTech Connect

Citation Information: Originator: Earth Science &Observation Center (ESOC), CIRES, University of Colorado at Boulder Originator: Geothermal Development Associates, Reno, Nevada Publication Date: 2012 Title: Archuleta Lineaments Edition: First Publication Information: Publication Place: Reno Nevada Publisher: Geothermal Development Associates, Reno, Nevada Description: This layer traces apparent topographic and air-photo lineaments in the area around Pagosa springs in Archuleta County, Colorado. It was made in order to identify possible fault and fracture systems that might be conduits for geothermal fluids. Geothermal fluids commonly utilize fault and fractures in competent rocks as conduits for fluid flow. Geothermal exploration involves finding areas of high near-surface temperature gradients, along with a suitable “plumbing system” that can provide the necessary permeability. Geothermal power plants can sometimes be built where temperature and flow rates are high. To do this, georeferenced topographic maps and aerial photographs were utilized in an existing GIS, using ESRI ArcMap 10.0 software. The USA_Topo_Maps and World_Imagery map layers were chosen from the GIS Server at server.arcgisonline.com, using a UTM Zone 13 NAD27 projection. This line shapefile was then constructed over that which appeared to be through-going structural lineaments in both the aerial photographs and topographic layers, taking care to avoid manmade features such as roads, fence lines, and right-of-ways. These lineaments may be displaced somewhat from their actual location, due to such factors as shadow effects with low sun angles in the aerial photographs. Note: This shape file was constructed as an aid to geothermal exploration in preparation for a site visit for field checking. We make no claims as to the existence of the lineaments, their location, orientation, and nature. Spatial Domain: Extent: Top: 4132831.990103 m Left: 311979.997741 m Right: 331678.289280 m Bottom: 4116067.165795 m Contact Information: Contact Organization: Geothermal Development Associates, Reno, Nevada Contact Person: Richard “Rick” Zehner Address: 3740 Barron Way City: Reno State: NV Postal Code: 89511 Country: USA Contact Telephone: 775-737-7806 Spatial Reference Information: Coordinate System: Universal Transverse Mercator (UTM) WGS’1984 Zone 13N False Easting: 500000.00000000 False Northing: 0.00000000 Central Meridian: -105.00000000 Scale Factor: 0.99960000 Latitude of Origin: 0.00000000 Linear Unit: Meter Datum: World Geodetic System 1984 (WGS ’1984) Prime Meridian: Greenwich Angular Unit: Degree Digital Form: Format Name: Shape file

Zehner, Richard E.

2012-01-01

217

Barriers to breastfeeding in the African American population of Shelby County, Tennessee.  

PubMed

Breastfeeding is recommended exclusively for the first 6 months of life, with continuation after the addition of complementary foods for at least 1 year of life. Breastfeeding rates are low in the Southeastern United States, especially among African Americans. Disparities in breastfeeding rates between African Americans and whites are especially pronounced in Memphis (Shelby County), TN. Our research objectives were to explore this disparity using focus groups, specifically to identify perceived barriers, and also to seek possible solutions from the target population. Focus groups were conducted in nine community settings within the county. Groups primarily consisted of women of childbearing years, but groups with men, grandmothers, and teens were also conducted. Common barriers for breastfeeding that were identified included pain, embarrassment with public nursing, going back to work, concern about "partying" and breastfeeding, and "just not wanting to" breastfeed. A notable finding was a substantial concern about sexuality and breastfeeding. As a possible solution, participants recommended putting breastfeeding educational materials widely across the county in many venues. Barriers to breastfeeding in Memphis are similar to those in other areas, with key concerns about sexuality and partying. Involving the target population yielded specific recommendations to improve breastfeeding promotion efforts. PMID:24972117

Ware, Julie L; Webb, Larita; Levy, Marian

2014-10-01

218

Geothermal development plan: Maricopa county  

SciTech Connect

Maricopa county is the area of Arizona receiving top priority since it contains over half of the state's population. The county is located entirely within the Basin and Range physiographic region in which geothermal resources are known to occur. Several approaches were taken to match potential users to geothermal resources. One approach involved matching some of the largest facilities in the county to nearby geothermal resources. Other approaches involved identifying industrial processes whose heat requirements are less than the average assessed geothermal reservoir temperature of 110/sup 0/C (230/sup 0/F). Since many of the industries are located on or near geothermal resources, geothermal energy potentially could be adapted to many industrial processes.

White, D.H.

1981-01-01

219

35. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. SPECIFICATIONS FOR BRIDGE OVER THE CEDAR RIVER AT SUTLIFF'S FERRY JOHNSON COUNTY, IOWA - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

220

33. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PLAN OF STONE PIER FOR EAST END, SUTLIFF'S FERRY BRIDGE, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

221

Analysis of Tn antigenicity with a panel of new IgM and IgG1 monoclonal antibodies raised against leukemic cells  

PubMed Central

CD175 or Tn antigen is a carbohydrate moiety of N-acetylgalactosamine (GalNAc)?1-O- linked to the residue of amino acid serine or threonine in a polypeptide chain. Despite the chemical simplicity of the Tn antigen, its antigenic structure is considered to be complex and the clear determinants of Tn antigenicity remain poorly understood. As a consequence, a broad variety of anti-Tn monoclonal antibodies (mAbs) have been generated. To further investigate the nature and complexity of the Tn antigen, we generated seven different anti-Tn mAbs of IgM and IgG classes raised against human Jurkat T cells, which are Tn-positive due to the low activity of T-synthase and mutation in specific chaperone Cosmc. The binding analysis of anti-Tn mAbs with the array of synthetic saccharides, glycopeptides and O-glycoproteins revealed unexpected differences in specificities of anti-Tn mAbs. IgM mAbs bound the terminal GalNAc residue of the Tn antigen irrespective of the peptide context or with low selectivity to the glycoproteins. In contrast, IgG mAbs recognized the Tn antigen in the context of a specific peptide motif. Particularly, JA3 mAb reacted to the GSPP or GSPAPP, and JA5 mAb recognized specifically the GSP motif (glycosylation sites are underlined). The major O-glycan carrier proteins CD43 and CD162 and isoforms of CD45 expressed on Jurkat cells were precipitated by anti-Tn mAbs with different affinities. In summary, our data suggest that Tn antigen–Ab binding capacity is determined by the peptide context of the Tn antigen, antigenic specificity of the Ab and class of the immunoglobulin. The newly generated anti-Tn IgG mAbs with the strong specificity to glycoprotein CD43 can be particularly interesting for the application in leukemia diagnostics and therapy. PMID:22143985

Blixt, Ola; Lavrova, Olga I; Mazurov, Dmitriy V; Cló, Emiliano; Kra?un, Stjepan K; Bovin, Nicolai V; Filatov, Alexander V

2012-01-01

222

33 CFR 100.905 - Door County Triathlon; Door County, WI.  

Code of Federal Regulations, 2011 CFR

... 1 2011-07-01 2011-07-01 false Door County Triathlon; Door County, WI. 100.905 Section 100.905 Navigation...SAFETY OF LIFE ON NAVIGABLE WATERS § 100.905 Door County Triathlon; Door County, WI. (a) Regulated Area. A regulated...

2011-07-01

223

33 CFR 100.905 - Door County Triathlon; Door County, WI.  

Code of Federal Regulations, 2014 CFR

... 1 2014-07-01 2014-07-01 false Door County Triathlon; Door County, WI. 100.905 Section 100.905 Navigation...SAFETY OF LIFE ON NAVIGABLE WATERS § 100.905 Door County Triathlon; Door County, WI. (a) Regulated Area. A regulated...

2014-07-01

224

33 CFR 100.905 - Door County Triathlon; Door County, WI.  

Code of Federal Regulations, 2012 CFR

... 1 2012-07-01 2012-07-01 false Door County Triathlon; Door County, WI. 100.905 Section 100.905 Navigation...SAFETY OF LIFE ON NAVIGABLE WATERS § 100.905 Door County Triathlon; Door County, WI. (a) Regulated Area. A regulated...

2012-07-01

225

33 CFR 100.905 - Door County Triathlon; Door County, WI.  

Code of Federal Regulations, 2010 CFR

... 1 2010-07-01 2010-07-01 false Door County Triathlon; Door County, WI. 100.905 Section 100.905 Navigation...SAFETY OF LIFE ON NAVIGABLE WATERS § 100.905 Door County Triathlon; Door County, WI. (a) Regulated Area. A regulated...

2010-07-01

226

33 CFR 100.905 - Door County Triathlon; Door County, WI.  

Code of Federal Regulations, 2013 CFR

... 1 2013-07-01 2013-07-01 false Door County Triathlon; Door County, WI. 100.905 Section 100.905 Navigation...SAFETY OF LIFE ON NAVIGABLE WATERS § 100.905 Door County Triathlon; Door County, WI. (a) Regulated Area. A regulated...

2013-07-01

227

76 FR 30152 - East Calloway County Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of...  

Federal Register 2010, 2011, 2012, 2013, 2014

...FRL-9310-4] East Calloway County Middle School Mercury Spill Site, Murray, Calloway County...the East Calloway County Middle School Mercury Spill Site located in Murray, Calloway...County [[Page 30153

2011-05-24

228

78 FR 21964 - Sheldon National Wildlife Refuge Humboldt and Washoe Counties, NV, and Lake County, OR; Record of...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Wildlife Refuge Humboldt and Washoe Counties, NV, and Lake County...remote area of northwest Nevada and southeast Oregon...ssquf] Humboldt County Public Library, 85 East...Winnemucca, NV. [ssquf] Washoe County Public Library,...

2013-04-12

229

RADIOCARBON MEASUREMENT OF THE BIOGENIC CONTRIBUTION TO SUMMERTIME PM 2.5 AMBIENT AEROSOL IN NASHVILLE, TN  

EPA Science Inventory

Radiocarbon (14C) measurements performed on PM-2.5 samples collected near Nashville, TN from June 21 to July 13, 1999, showed high levels of modern carbon, ranging from 56 to 80% of the total carbon in the samples. Radiocarbon measurements performed on dichloromethane extracts of...

230

Cloning, characterization, and complementation of lesions causing acid sensitivity in Tn5-induced mutants of Rhizobium meliloti WSM419.  

PubMed Central

Four Tn5-induced mutants of Rhizobium meliloti WSM419 were unable to grow or maintain intracellular pH at an external pH of 5.6. Restriction analysis of DNA fragments carrying Tn5 and flanking sequences cloned from these mutants indicated that all four cloned mutations are unique and that the two strains (TG1-6 and TG1-11) carry Tn5 insertions which are within 4.4 kilobases of each other on a single EcoRI fragment. Southern analysis of total mutant DNA indicated a single copy of Tn5 in each mutant. A limited cosmid gene bank of wild-type WSM419 DNA was probed for homology to mutant DNA cloned from the acid-sensitive mutants. Dot hybridization experiments identified one cosmid element within this bank carrying wild-type DNA sequences corresponding to DNA implicated in acid tolerance. This cosmid was able to complement defects in growth and intracellular pH maintenance in TG1-11 but not TG1-6. PMID:2168374

Goss, T J; O'Hara, G W; Dilworth, M J; Glenn, A R

1990-01-01

231

Diophantine equations with products of consecutive terms in Lucas sequences F. Luca and T.N. Shorey  

E-print Network

n ) n#0 and (v n ) n#0 are the sequences of Fibonacci and Lucas numbers denoted from here on by (F nDiophantine equations with products of consecutive terms in Lucas sequences F. Luca and T.N. Shorey the product of consecutive terms in arithmetic progressions is replaced by the product of terms in Lucas

Shorey, T.N.

232

International Composites Expo ICE-98 Nashville, TN, January 19-21, 1998. Title: Strength, Durability and Health Monitoring of Composites  

E-print Network

1 International Composites Expo ICE-98 Nashville, TN, January 19-21, 1998. Title: Strength, Durability and Health Monitoring of Composites Overlays on Civil Engineering Structures Authors: Victor and health monitoring aspects of composite overlays on civil engineering structures are discussed

Giurgiutiu, Victor

233

Minimization of the Escherichia coli genome using a Tn5-targeted Cre\\/loxP excision system  

Microsoft Academic Search

An increasing number of microbial genomes have been completely sequenced, and functional analyses of these genomic sequences are under way. To facilitate these analyses, we have developed a genome-engineering tool for determining essential genes and minimizing bacterial genomes. We made two large pools of independent transposon mutants in Escherichia coli using modified Tn5 transposons with two different selection markers and

Byung Jo Yu; Bong Hyun Sung; Michael D. Koob; Choong Hoon Lee; Jun Hyoung Lee; Won Sik Lee; Mi Sun Kim; Sun Chang Kim

2002-01-01

234

Arbitrary PCR for Rapid Mapping of Tn5 Insertions in Pyoverdine Genes of Pseudomonas fluorescens Pf-5  

Technology Transfer Automated Retrieval System (TEKTRAN)

A collection of 13 transposon mutants deficient in pyoverdine production was analyzed using an arbitrary polymerase chain reaction (PCR) approach to map the sites of Tn5 insertions in the genome of Pseudomonas fluorescens Pf-5. The arbitrary PCR method involved two rounds of reactions, with the fi...

235

Superfund Record of Decision (EPA Region 4): Murray-Ohio Dump, Operable Unit 1, Lawrence County, Lawrenceburg, TN, June 17, 1994  

SciTech Connect

This decision document represents the selected remedial action for the Murray Ohio Dump Site. This final remedy addresses remediation of ground-water/seep contamination and soil contamination by eliminating or reducing the risks posed by the Site, through treatment, engineering and institutional controls.

NONE

1995-02-01

236

Mobility and Generation of Mosaic Non-Autonomous Transposons by Tn3-Derived Inverted-Repeat Miniature Elements (TIMEs)  

PubMed Central

Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons – Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element “captured” with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not only of transposons and plasmids, but also of other types of mobile genetic elements. PMID:25121765

Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

2014-01-01

237

Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs).  

PubMed

Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons--Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element "captured" with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not only of transposons and plasmids, but also of other types of mobile genetic elements. PMID:25121765

Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

2014-01-01

238

24 CFR 570.307 - Urban counties.  

Code of Federal Regulations, 2010 CFR

...DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMMUNITY FACILITIES...Entitlement Grants § 570.307 Urban counties. (a) Determination...counties to receive entitlements as urban counties upon receipt of qualification...time, and in such manner and form as prescribed by...

2010-04-01

239

Industry-Education-Business: Wyandotte County Survey.  

ERIC Educational Resources Information Center

This study was undertaken to determine the advisability of establishing an area vocational-technical school. The county's population characteristics and the economic base of the county as well as its history were studied for background information. the staff then surveyed 2,288 high school seniors in the county to determine which ones and how many…

Kansas City Public Schools, KS.

240

Tennessee Higher Education County Profiles, 2009  

ERIC Educational Resources Information Center

This report presents a localized perspective on Tennessee higher education for the year 2009, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; and (4) number of county residents participating in the state's primary need-based and merit-based financial aid…

Tennessee Higher Education Commission, 2009

2009-01-01

241

Public Health County of Santa Cruz  

E-print Network

Public Health Division County of Santa Cruz HEALTH SERVICES AGENCY POST OFFICE BOX 962, 1080 Date: December 3, 2009 Phone: 831-454-4000 Email: hsadocpio@co.santa-cruz.ca.us County Health Department Announces Flu Vaccine Clinic Santa Cruz County Public Health will hold an additional walk

California at Santa Cruz, University of

242

Genessee County Birth-to-Work  

E-print Network

Genessee County Birth-to-Work September 2010 #12;Genesee County Birth to Work It and disparities #12;Genesee County Birth to Work Birth to Work is about all of us building and transforming our communities to effectively compete and prosper in the global knowledge economy #12;Genesee Birth to Work

243

Functional Tn5393-Like Transposon in the R Plasmid pRAS2 from the Fish Pathogen Aeromonas salmonicida subspecies salmonicida Isolated in Norway  

PubMed Central

Tn5393c containing strA-strB was identified as part of R plasmid pRAS2 from the fish pathogen Aeromonas salmonicida subsp. salmonicida. This is the first time an intact and active transposon in the Tn5393 family has been reported in an ecological niche other than an agricultural habitat. PMID:11097945

L'Abée-Lund, Trine M.; Sørum, Henning

2000-01-01

244

PHYSICAL LINKAGE OF MULTIPLE DRUG RESISTANCE TRANSPOSON TN21 TO COLICIN PLASMIDS AND DISINFECTANT RESISTANCE GENE MERB IN AVIAN ESCHERICHIA COLI  

Technology Transfer Automated Retrieval System (TEKTRAN)

Recently, we have reported on the widespread distribution of multiple-drug resistance transposon, Tn21 among clinical, avian Escherichia coli isolates in North Georgia. Tn21 is a 21 kb transposon that contains an integron, a mobile genetic element that “captures” antibiotic resistance gene(s), strep...

245

Identification of a circular intermediate in the transfer and transposition of Tn4555, a mobilizable transposon from Bacteroides spp.  

PubMed Central

Transmissible cefoxitin (FX) resistance in Bacteroides vulgatus CLA341 was associated with the 12.5-kb, mobilizable transposon, Tn4555, which encoded the beta-lactamase gene cfxA. Transfer occurred by a conjugation-like mechanism, was stimulated by growth of donor cells with tetracycline (TC), and required the presence of a Bacteroides chromosomal Tcr element. Transconjugants resistant to either FX, TC, or both drugs were obtained, but only Fxr Tcr isolates could act as donors of Fxr in subsequent matings. Transfer of Fxr could be restored in Fxr Tcs strains by the introduction of a conjugal Tcr element from Bacteroides fragilis V479-1. A covalently closed circular DNA form of Tn4555 was observed in donor cells by Southern hybridization, and the levels of this circular transposon increased significantly in cells grown with TC. Both the cfxA gene and the Tn4555 mobilization region hybridized to the circular DNA, suggesting that this was a structurally intact transposon unit. Circular transposon DNA purified by CsCl-ethidium bromide density gradient centrifugation was used to transform Tcs B. fragilis 638, and Fxr transformants were obtained. Both the circular form and the integrated Tn4555 were observed in transformants, but the circular form was present at less than one copy per chromosomal equivalent. Examination of genomic DNA from Fxr transformants and transconjugants revealed that Tn4555 could insert at a wide variety of chromosomal sites. Multiple transposon insertions were present in many of the transconjugants, indicating that there was no specific barrier to the introduction of a second transposon copy. Images PMID:8386723

Smith, C J; Parker, A C

1993-01-01

246

Robust rat pulmonary radioprotection by a lipophilic Mn N-alkylpyridylporphyrin, MnTnHex-2-PyP5+?  

PubMed Central

With the goal to enhance the distribution of cationic Mn porphyrins within mitochondria, the lipophilic Mn(III)meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin, MnTnHex-2-PyP5+ has been synthesized and tested in several different model of diseases, where it shows remarkable efficacy at as low as 50 µg/kg single or multiple doses. Yet, in a rat lung radioprotection study, at higher 0.6–1 mg/kg doses, due to its high accumulation and micellar character, it became toxic. To avoid the toxicity, herein the pulmonary radioprotection of MnTnHex-2-PyP5+ was assessed at 50 µg/kg. Fischer rats were irradiated to their right hemithorax (28 Gy) and treated with 0.05 mg/kg/day of MnTnHex-2-PyP5+ for 2 weeks by subcutaneously-implanted osmotic pumps, starting at 2 h post-radiation. The body weights and breathing frequencies were followed for 10 weeks post-radiation, when the histopathology and immunohistochemistry were assessed. Impact of MnTnHex-2-PyP5+ on macrophage recruitment (ED-1), DNA oxidative damage (8-OHdG), TGF-?1, VEGF(A) and HIF-1? were measured. MnTnHex-2-PyP5+ significantly decreased radiation-induced lung histopathological (H&E staining) and functional damage (breathing frequencies), suppressed oxidative stress directly (8-OHdG), or indirectly, affecting TGF-?1, VEGF (A) and HIF-1? pathways. The magnitude of the therapeutic effects is similar to the effects demonstrated under same experimental conditions with 120-fold higher dose of ~5000-fold less lipophilic Mn(III)meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin, MnTE-2-PyP5+. PMID:24624330

Gauter-Fleckenstein, Benjamin; Reboucas, Julio S.; Fleckenstein, Katharina; Tovmasyan, Artak; Owzar, Kouros; Jiang, Chen; Batinic-Haberle, Ines; Vujaskovic, Zeljko

2014-01-01

247

The Licking County Writing Project.  

ERIC Educational Resources Information Center

The 24 articles in this special journal resulted from the Licking County (Ohio) Writing Project. The articles provide information about the following: (1) how to motivate students and teachers, (2) prewriting activities, (3) lightening the teacher's grading load, (4) the uses of conferences, (5) sustained silent reading, (6) traditional techniques…

Davis, James E., Ed.; Davis, Hazel K., Ed.

1982-01-01

248

Gallatin County Secondary Data Analysis  

E-print Network

prevalence (Heart Attack) 2.4% 4.1% 6.0% All Sites Cancer 416.6 (Region 4) 455.5 543.2 1 Community, Powell, Deer Lodge, Jefferson, Broadwater, Meagher, Silver Bow, Gallatin, Park, Madison, and Beaverhead Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease

Maxwell, Bruce D.

249

Beaverhead County Secondary Data Analysis  

E-print Network

prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT American Diabetes Association (2012) Region 4 (Southwest) ­ Lewis and Clark, Granite, Powell, Deer Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2

Maxwell, Bruce D.

250

Meagher County Secondary Data Analysis  

E-print Network

prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT American Diabetes Association (2012) Region 4 (Southwest) ­ Lewis and Clark, Granite, Powell, Deer Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease

Maxwell, Bruce D.

251

Broadwater County Secondary Data Analysis  

E-print Network

prevalence 1.9% 2.5% 2.6% Diabetes prevalence 5.1% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Association (2012) Region 4 (Southwest): Lewis and Clark, Granite, Powell, Deer Lodge, Jefferson, Broadwater Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3

Maxwell, Bruce D.

252

Jefferson County Secondary Data Analysis  

E-print Network

prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT American Diabetes Association (2012) Region 4 (Southwest) ­ Lewis and Clark, Granite, Powell, Deer Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease

Maxwell, Bruce D.

253

Powell County Secondary Data Analysis  

E-print Network

Infarction prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community, Powell, Deer Lodge, Jefferson, Broadwater, Meagher, Silver Bow, Gallatin, Park, Madison, and Beaverhead Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2

Maxwell, Bruce D.

254

Madison County Secondary Data Analysis  

E-print Network

prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT American Diabetes Association (2012) Region 4 (Southwest) ­ Lewis and Clark, Granite, Powell, Deer Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer

Maxwell, Bruce D.

255

Fresno County Mock Trial Competition.  

ERIC Educational Resources Information Center

THE FOLLOWING IS THE FULL TEXT OF THIS DOCUMENT: The Fresno County Office of Education and the Fresno Unified School District hosted the Mock Trial Competition. The state competition is sponsored by the Constitutional Rights Foundation, with cosponsorship from the California State Bar Association and the California Young Lawyer's Association. This…

Fresno City Unified School District, CA.

256

STATE OF TEXAS COUNTY OF ________________  

E-print Network

AFFIDAVIT STATE OF TEXAS § § COUNTY OF ________________ § Before me, the undersigned Notary Public and correct. 2. I graduated or will graduate from a Texas high school or received my GED certificate in Texas. 3. I resided in Texas for three years leading up to graduation from high school or receiving my GED

Garbey, Marc

257

Daniels County Secondary Data Analysis  

E-print Network

,914 Unemployment Rate 7 3.2% 6.3% 7.7% Persons Below Poverty Level 1 18.0% 14.0% 13.8% Uninsured Adults (Age .9% (Region 1) 20.7% 0 5 10 15 20 25 30 35 40 45 (%) Education Level County Montana 10 Indicators Northwest.7 Not relevant Age 1 Gender 1 Male Female

Maxwell, Bruce D.

258

Carter County Secondary Data Analysis  

E-print Network

.7% Persons Below Poverty Level1 18.0% 14.0% 13.8% Uninsured Adults (Age 10 15 20 25 30 35 40 45 (%) Education Level County Montana 10 Indicators Northwest, Imp. % 7% 62% 13% Gender1 Male Female Male Female Male Female 48.9% 51.1% 50.1% 49.9% 49.2% 50

Maxwell, Bruce D.

259

Chouteau County Secondary Data Analysis  

E-print Network

,914 Unemployment Rate 7 4.0% 6.3% 7.7% Persons Below Poverty Level 1 20.0% 14.0% 13.8% Uninsured Adults (Age .6% (Region 2) 20.7% 0 5 10 15 20 25 30 35 40 (%) Education Level County Montana 10 Indicators Northwest, Imp.7 Not relevant Age 1 Gender 1 Male Female

Maxwell, Bruce D.

260

Dawson County Secondary Data Analysis  

E-print Network

,000 $51,914 Unemployment Rate7 3.5% 6.3% 7.7% Persons Below Poverty Level1 14.0% 14.0% 13 (%) Education Level County Montana 10 Indicators Northwest, 65+ 6% 62% 18% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.8% 50

Maxwell, Bruce D.

261

Fergus County Secondary Data Analysis  

E-print Network

.7% Persons Below Poverty Level1 15.0% 14.0% 13.8% Uninsured Adults (Age .7% 0 5 10 15 20 25 30 35 40 (%) Education Level County Montana 10 Indicators Northwest, Imp. Graph% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.1% 50.9% 50.1% 49.9% 49.2% 50

Maxwell, Bruce D.

262

Custer County Secondary Data Analysis  

E-print Network

.7% Persons Below Poverty Level1 15.0% 14.0% 13.8% Uninsured Adults (Age .7% 0 5 10 15 20 25 30 35 40 (%) Education Level County Montana 10 Indicators Northwest% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.1% 50.9% 50.1% 49.9% 49.2% 50

Maxwell, Bruce D.

263

Yellowstone County Secondary Data Analysis  

E-print Network

,000 $51,914 Unemployment Rate7 4.5% 6.3% 7.7% Persons Below Poverty Level1 11.0% 14.0% 13 (%) Education Level County Montana 10 Indicators Northwest, Imp. Graph (20 65+ 7% 62% 14% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.1% 50

Maxwell, Bruce D.

264

Toole County Secondary Data Analysis  

E-print Network

$37,959 $43,000 $51,914 Unemployment Rate7 4.6% 6.3% 7.7% Persons Below Poverty Level1 12.7% 0 5 10 15 20 25 30 35 40 (%) Education Level County Montana 10 Indicators Northwest, Imp. Graph 1864 65+ Gender1 Male Female Male Female Male

Maxwell, Bruce D.

265

Retrofit Savings for Brazos County  

E-print Network

This report presents the energy and dollar savings for the period May 2000 - April 2001 for 10 of the Brazos County facilities that have been retrofit. The electricity use saved was 555,170 kWh and the demand was 1062 kW, which is equivalent to a...

Baltazar-Cervantes, J. C.; Shao, X.; Claridge, D. E.

2001-01-01

266

Hydrogeology of Webb County, Texas  

USGS Publications Warehouse

Introduction: Webb County, in semiarid South Texas on the U.S.-Mexico border, is a region confronted by increasing stresses on natural resources. Laredo (fig. 1), the largest city in Webb County (population 193,000 in 2000), was one of the 10 fastest-growing metropolitan areas in the country during 1990-2000 (Perry and Mackun, 2001). Commercial and industrial activities have expanded throughout the region to support the maquiladora industry (manufacturing plants in Mexico) along the border and other growth as a result of the passage of the North American Free Trade Agreement. The Rio Grande currently (2002) is the primary source of public water supply for Laredo and other cities along the border in Webb County (fig. 1). Other cities, such as Bruni and Mirando City in the southeastern part of the county, rely on ground-water supplies to meet municipal demands. Increased water demand associated with development and population growth in the region has increased the need for the City of Laredo and Webb County to evaluate alternative water sources to meet future demand. Possible options include (1) supplementing the surface-water supply with ground water, and (2) applying artificial storage and recovery (ASR) technology to recharge local aquifers. These options raise issues regarding the hydraulic capability of the aquifers to store economically substantial quantities of water, current or potential uses of the resource, and possible effects on the quality of water resulting from mixing ground water with alternative source waters. To address some of these issues, the U.S. Geological Survey (USGS), in cooperation with the City of Laredo, began a study in 1996 to assess the ground-water resources of Webb County. A hydrogeologic study was conducted to review and analyze available information on the hydrogeologic units (aquifers and confining units) in Webb County, to locate available wells in the region with water-level and water-quality information from the aquifers, and to analyze the hydraulic properties of the aquifers. The purpose of this report is to document the findings of the study. The information is organized by hydrogeologic unit and presented on this and six other sheets.

Lambert, Rebecca B.

2004-01-01

267

Sialyl-Tn in Cancer: (How) Did We Miss the Target?  

PubMed Central

Sialyl-Tn antigen (STn) is a short O-glycan containing a sialic acid residue ?2,6-linked to GalNAc?-O-Ser/Thr. The biosynthesis of STn is mediated by a specific sialyltransferase termed ST6GalNAc I, which competes with O-glycans elongating glycosyltransferases and prevents cancer cells from exhibiting longer O-glycans. While weakly expressed by fetal and normal adult tissues, STn is expressed by more than 80% of human carcinomas and in all cases, STn detection is associated with adverse outcome and decreased overall survival for the patients. Because of its pan-carcinoma expression associated with an adverse outcome, an anti-cancer vaccine, named Theratope, has been designed towards the STn epitope. In spite of the great enthusiasm around this immunotherapy, Theratope failed on Phase III clinical trial. However, in lieu of missing this target, one should consider to revise the Theratope design and the actual facts. In this review, we highlight the many lessons that can be learned from this failure from the immunological standpoint, as well as from the drug design and formulation and patient selection. Moreover, an irrefutable knowledge is arising from novel immunotherapies targeting other carbohydrate antigens and STn carrier proteins, such as MUC1, that will warrantee the future development of more successful anti-STn immunotherapy strategies. PMID:24970145

Julien, Sylvain; Videira, Paula A.; Delannoy, Philippe

2012-01-01

268

Understanding the structural specificity of Tn antigen for its receptor: an NMR solution study.  

PubMed

The present work aims at understanding the structural basis of the biological recognition of Tn antigen (GalNAc-?-O-L-Ser), a specific epitope expressed by tumor cells, and the role of its amino acidic moiety in the interaction with its receptor (the isolectin B4 extracted from Vicia villosa). An NMR structural characterization of the ? and ? anomers, based on J couplings and molecular modeling revealed a structure in very good agreement with data reported in literature for variants of the same molecules. In order to demonstrate the involvement of the amino acid in the ligand-receptor recognition, also GalNAc-?-O-D-Ser was studied; the change in the stereochemistry is in fact expected to impact on the interaction only in case the serine is part of the epitope. Relaxation properties in the presence of the receptor clearly indicated a selective recognition of the natural L form, probably due to the formation of a water-mediated hydrogen bond with Asn 129 of the protein. PMID:22341503

D'Amelio, Nicola; Coslovi, Anna; Rossi, Marco; Uggeri, Fulvio; Paoletti, Sergio

2012-04-01

269

Origin and structural implications of upper Miocene rhyolites in Kingston Canyon, Piute County, Utah.  

USGS Publications Warehouse

Kingston Canyon is one of the deepest antecedent canyons in the High Plateaus subprovince of the Colorado Plateaus. Here the E Fork of the Sevier River flows westward transversely across the gently E tilted Sevier Plateau, which is developed on a basin-range fault block uplifted more than 1500m along the Sevier fault zone on the W. Upper Tertiary rhyolites, uncommon in SW Utah, occur both on the northern rim and in the bottom of Kingston Canyon. Those on the northern rim consist of lava flows and volcanic domes of the rhyolite of Forshea Mountain, dated by K/Ar methods at 7.6Ma old. Those in the bottom of Kingston Canyon, the rhyolite of Phonolite Hill, are especially well exposed and provide spectacular examples of a pyroclastic cone whose base is about at river level and a steep-sided volcanic dome emplaced into and through these deposits. The pyroclastic deposits, formerly 500 or more metres thick, consist of airfall, mudflow, and ash-flow(?) material of rhyolite and foreign lithic fragments especially olivine basalt. The dome consists of flow-banded, mostly devitrified rhyolite as much as 500m thick; it has been dated by K/Ar methods at 5.4Ma. In addition to the rhyolites, a dome and lava-flow complex, the rhyodacite of Dry Lake, occurs near the northern rim and is considered to postdate the rhyolite of Forshea Mountain and predate the rhyolite of Phonolite Hill. -from Authors

Rowley, P.D.; Steven, T.A.; Mehnert, H.H.

1981-01-01

270

Analysis of a Clostridium difficile PCR ribotype 078 100 kilobase island reveals the presence of a novel transposon, Tn6164  

PubMed Central

Background Clostridium difficile is the main cause of antibiotic associated diarrhea. In the past decade, the number of C. difficile patients has increased dramatically, coinciding with the emergence of two PCR ribotypes 027 and 078. PCR ribotype 078 is also frequently found during C. difficile outbreaks in pigfarms. Previously, the genome of the PCR ribotype 078 strain M120, a human isolate, was described to contain a unique insert of 100 kilobases. Results Analysis of this insert revealed over 90 open reading frames, encoding proteins originating from transposons, phages and plasmids. The insert was shown to be a transposon (Tn6164), as evidenced by the presence of an excised and circularised molecule, containing the ligated 5’and 3’ends of the insert. Transfer of the element could not be shown through filter-mating experiments. Whole genome sequencing of PCR ribotype 078 strain 31618, isolated from a diarrheic piglet, showed that Tn6164 was not present in this strain. To test the prevalence of Tn6164, a collection of 231 Clostridium difficile PCR ribotype 078 isolates from human (n = 173) and porcine (n = 58) origin was tested for the presence of this element by PCR. The transposon was present in 9 human, tetracycline resistant isolates, originating from various countries in Europe, and none of the pig strains. Nine other strains, also tetracycline resistant human isolates, contained half of the transposon, suggesting multiple insertion steps yielding the full Tn6164. Other PCR ribotypes (n = 66) were all negative for the presence of the transposon. Multi locus variable tandem repeat analysis revealed genetic relatedness among transposon containing isolates. Although the element contained several potential antibiotic resistance genes, it did not yield a readily distinguishable phenotype. Conclusions Tn6164 is a newly described transposon, occurring sporadically in C. difficile PCR ribotype 078 strains. Although no transfer of the element could be shown, we hypothesize that the element could serve as a reservoir of antibiotic resistance genes for other bacteria. Further research is needed to investigate the transfer capabilities of the element and to substantiate the possible role of Tn6164 as a source of antibiotic resistance genes for other gut pathogens. PMID:22747711

2012-01-01

271

Monroe County Library System: Pathfinders  

NSDL National Science Digital Library

Named after President James Monroe, Monroe County is part of upstate New York. The industrial metropolis of Rochester sits within its boundaries, and one of its key institutions is the Monroe County Library System. Over the past several years, the System has created this treasure trove of digital collections which dissect and organize the history of the area through historic maps, architectural guides, and an online history tour of places in Rochester that played a role in the abolition movement and the Civil War. First-time visitors might want to start out by looking over the "Raising the Flag: Patriotism in Rochester, 1892-1922" photo gallery and then move on the Susan B. Anthony Letters Collection. Library staff members have also created nine fine research guides for scholars and others, and they include a guide to Rochester immigrants and a house history guide.

272

SEMINOLE COUNTY GOVERNMENT AGENDA MEMORANDUM  

Microsoft Academic Search

MOTION\\/RECOMMENDATION: Approve to submit a grant application to the Substance Abuse and Mental Health Services Administration requesting up to $900,000.00 in funding through their Adult Treatment Drug Court Program; and authorize the County Manager to execute supporting documents as may be required for the application. BACKGROUND: The Substance Abuse and Mental Health Services Administration (SAMHSA) is accepting applications to expand

Lisa Spriggs; Jennifer Bero; Michele Saunders

2009-01-01

273

Valley County Secondary Data Analysis  

E-print Network

Rate7 4.4% 6.3% 7.7% Persons Below Poverty Level1 13.0% 14.0% 13.8% Uninsured Adults (Age time for physical activity1 27.9% 20.7% 0 5 10 15 20 25 30 35 40 (%) Education Level County Montana% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.3% 50.7% 50.1% 49.9% 49.2% 50

Maxwell, Bruce D.

274

Stillwater County Secondary Data Analysis  

E-print Network

7 5.2% 6.3% 7.7% Persons Below Poverty Level 1 10.0% 14.0% 13.8% Uninsured Adults (Age (%) Education Level County Montana 10 Indicators Northwest, Imp. Graph (20% 62% 16% 6% 63% 14% 7% 62% 13% Gender 1 Male Female Male Female Male Female 51.4% 48.6% 50.1% 49.9% 49

Maxwell, Bruce D.

275

Musselshell County Secondary Data Analysis  

E-print Network

Rate7 5.7% 6.3% 7.7% Persons Below Poverty Level1 20.0% 14.0% 13.8% Uninsured Adults (Age time for physical activity1 22.0% 20.7% 0 5 10 15 20 25 30 35 40 45 50 (%) Education Level County% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 48.6% 51.4% 50.1% 49.9% 49

Maxwell, Bruce D.

276

Wibaux County Secondary Data Analysis  

E-print Network

Rate7 2.8% 6.3% 7.7% Persons Below Poverty Level1 15.0% 14.0% 13.8% Uninsured Adults (Age for physical activity1 27.9% 20.7% 0 5 10 15 20 25 30 35 40 (%) Education Level County Montana 10 65+ 3% 58% 24% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 47.9% 52

Maxwell, Bruce D.

277

Polyvalency of Tn (GalNAcalpha1-->Ser/Thr) glycotope as a critical factor for Vicia villosa B4 and glycoprotein interactions.  

PubMed

Vicia villosa B(4) (VVL-B(4)) is an important lectin for detecting exposed Tn (GalNAcalpha1-Ser/Thr) determinants on cancer cells. In order to elucidate the binding factors involved in VVL-B(4) and glycotope interaction, the binding properties of this lectin were analyzed by enzyme-linked lectinosorbent and inhibition assays. From the results, it is concluded that the most critical factor affecting VVL-B(4) binding is polyvalency at the alpha anomer of Gal with -NH CH(3)CO at carbon-2 (Tn epitope), which enhances the reactivity by 3.3x10(5) times over monovalent Gal. The reactivities of glycotopes can be ranked as follows: high density Tn cluster >Tn glycopeptides (MW<3.0x10(3) > monomeric Tn to tri- Tn glycopeptides > other GalNAcalpha/beta-related structural units>Gal and Galalpha- or beta-linked ligands, demonstrating the essential role of the polyvalency of Tn glycotopes in the enhancement of the binding. PMID:15044001

Wu, Albert M

2004-03-26

278

Bacteroides fragilis Transfer Factor Tn5520: the Smallest Bacterial Mobilizable Transposon Containing Single Integrase and Mobilization Genes That Function in Escherichia coli  

PubMed Central

Many bacterial genera, including Bacteroides spp., harbor mobilizable transposons, a class of transfer factors that carry genes for conjugal DNA transfer and, in some cases, antibiotic resistance. Mobilizable transposons are capable of inserting into and mobilizing other, nontransferable plasmids and are implicated in the dissemination of antibiotic resistance. This paper presents the isolation and characterization of Tn5520, a new mobilizable transposon from Bacteroides fragilis LV23. At 4,692 bp, it is the smallest mobilizable transposon reported from any bacterial genus. Tn5520 was captured from B. fragilis LV23 by using the transfer-deficient shuttle vector pGAT400?BglII. The termini of Tn5520 contain a 22-bp imperfect inverted repeat, and transposition does not result in a target site repeat. Tn5520 also demonstrates insertion site sequence preferences characterized by A-T-rich nucleotide sequences. Tn5520 has been sequenced in its entirety, and two large open reading frames whose predicted protein products exhibit strong sequence similarity to recombinase-integrase enzymes and mobilization proteins, respectively, have been identified. The transfer, mobilization, and transposition properties of Tn5520 have been studied, revealing that Tn5520 mobilizes plasmids in both B. fragilis and Escherichia coli at high frequency and also transposes in E. coli. PMID:10198023

Vedantam, Gayatri; Novicki, Thomas J.; Hecht, David W.

1999-01-01

279

Identification of Genes Involved in Swarming Motility Using a Pseudomonas aeruginosa PAO1 Mini-Tn5-lux Mutant Library?  

PubMed Central

During a screening of a mini-Tn5-luxCDABE transposon mutant library of Pseudomonas aeruginosa PAO1 for alterations in swarming motility, 36 mutants were identified with Tn5 insertions in genes for the synthesis or function of flagellin and type IV pilus, in genes for the Xcp-related type II secretion system, and in regulatory, metabolic, chemosensory, and hypothetical genes with unknown functions. These mutants were differentially affected in swimming and twitching motility but in most cases had only a minor additional motility defect. Our data provide evidence that swarming is a more complex type of motility, since it is influenced by a large number of different genes in P. aeruginosa. Conversely, many of the swarming-negative mutants also showed an impairment in biofilm formation, indicating a strong relationship between these types of growth states. PMID:17158671

Overhage, Joerg; Lewenza, Shawn; Marr, Alexandra K.; Hancock, Robert E. W.

2007-01-01

280

Specific Binding of Integrase to the Origin of Transfer (oriT) of the Conjugative Transposon Tn916  

PubMed Central

Purified integrase protein (Int) of the conjugative transposon Tn916 was shown, using nuclease protection experiments, to bind specifically to a site within the origin of conjugal transfer of the transposon, oriT. A sequence similar to the ends of the transposon that are bound by the C-terminal DNA-binding domain of Int was present in the protected region. However, Int binding to oriT required both the N- and C-terminal DNA-binding domains of Int, and the pattern of nuclease protection differed from that observed when Int binds to the transposon ends and flanking DNA. Binding of Int to oriT may be part of a mechanism to prevent premature conjugal transfer of Tn916 prior to excision from the donor DNA. PMID:11292817

Hinerfeld, Douglas; Churchward, Gordon

2001-01-01

281

The Transfer Origin for Bacteroides Mobilizable Transposon Tn4555 Is Related to a Plasmid Family from Gram-Positive Bacteria  

PubMed Central

Conjugal transfer of Bacteroides mobilizable transposon Tn4555 was examined with an Escherichia coli-based assay system. It was shown that mobilization required the cis-acting oriTTn region and that the Tn4555 mobATn gene and RK231 must be present in trans. With alkaline agarose gel electrophoresis and filter blot hybridizations, it was shown that at oriTTn there was a site- and strand-specific cleavage event that was dependent on mobATn. The 5? end of this cleavage site was mapped by primer extension, and the nucleotide sequence surrounding the site had homology to a family of oriT nick sites found in mobilizable plasmids of gram-positive bacteria. Removal of the nick site by deletion of 18 bp surrounding the site resulted in a significant loss of transfer activity. PMID:9440538

Smith, C. Jeffrey; Parker, Anita C.

1998-01-01

282

Surface modifications in the platelets of a patient with alpha-N-acetyl-D-galactosamine residues, the Tn-syndrome.  

PubMed Central

The Tn-syndrome is an acquired disorder characterized by the polyagglutination of blood cells and the pathological exposure of alpha-N-acetyl-D-galactosamine residues (Tn-antigen) at the cell surface. We now report studies on the platelet of a patient (Ba.) of which 81% reacted positively with a fluorescein conjugate of Helix pomatia agglutinin (HPA). The surface proteins of Ba. platelets were labeled with 125I by the lactoperoxidase-catalyzed procedure; single and two-dimensional electrophoresis on sodium dodecyl sulfate (SDS)-polyacrylamide gels was followed by autoradiography that revealed normal 125I-labeling of the major membrane glycoproteins (GP) but that GP Ib had a faster than normal migration. the abnormal GP Ib of Ba. platelets was strongly labeled when platelet suspensions were treated sequentially with neuraminidase, galactose oxidase, and sodium [3H]borohydride. Unlike the GP Ib of normal human platelets, it was also strongly labeled when Ba. platelets were treated with galactose oxidase and sodium [3H]borohydride alone. Both the alloantigen, PlA1, and quinidine-dependent antibody receptor activity were normally expressed by Ba. platelets, which also bound a monoclonal antibody (AN51) to GP Ib. Analysis of Ba. platelets by crossed immunoelectrophoresis using a rabbit anti-human platelet antibody preparation revealed the presence of an immunoprecipitate in the GP Ib position that had an abnormal appearance and migration in the second dimension. An altered position of the precipitate given by Factor VIIIR:Ag was also noted. Incorporation of HPA into the agarose gel during the first dimension electrophoresis resulted in the specific precipitation of the abnormal GP Ib of Ba. platelets. Our studies show that circulating Tn-platelets contain GP Ib with a modified oligosaccharide chain structure responsible for the platelet expression of Tn-antigen activity. Images PMID:7174794

Nurden, A T; Dupuis, D; Pidard, D; Kieffer, N; Kunicki, T J; Cartron, J P

1982-01-01

283

Altered expression of sialyl-Tn, Lewis antigens and carcinoembryonic antigen between primary and metastatic lesions of uterine cervical cancers  

Microsoft Academic Search

Immunohistochemical examination was performed of serial sections of 24 normal human adult cervical tissues and 53 human cervical carcinomas including 36 cases with lymph node metastasis. For this investigation, monoclonal antibodies directed to Lewis-X, Lewis-Y, sialyl-dimeric Lewis-X (SDLX), sialyl-Tn (STn) and carcinoembryonic antigen (CEA) were used. STn and CEA antigens were expressed very weakly in the normal cervical epithelium but

H. Ogawa; M. Inoue; O. Tanizawa; M. Miyamoto; M. Sakurai

1992-01-01

284

Targeting of T/Tn Antigens with a Plant Lectin to Kill Human Leukemia Cells by Photochemotherapy  

PubMed Central

Photochemotherapy is used both for solid tumors and in extracorporeal treatment of various hematologic disorders. Nevertheless, its development in oncology remains limited, because of the low selectivity of photosensitizers (PS) towards human tumor cells. To enhance PS efficiency, we recently covalently linked a porphyrin (TrMPyP) to a plant lectin (Morniga G), known to recognize with high affinity tumor-associated T and Tn antigens. The conjugation allowed a quick uptake of PS by Tn-positive Jurkat leukemia cells and efficient PS-induced phototoxicity. The present study was performed: (i) to evaluate the targeting potential of the conjugate towards tumor and normal cells and its phototoxicity on various leukemia cells, (ii) to investigate the mechanism of conjugate-mediated cell death. The conjugate: (i) strongly increased (×1000) the PS phototoxicity towards leukemic Jurkat T cells through an O-glycan-dependent process; (ii) specifically purged tumor cells from a 1?1 mixture of Jurkat leukemia (Tn-positive) and healthy (Tn-negative) lymphocytes, preserving the activation potential of healthy lymphocytes; (iii) was effective against various leukemic cell lines with distinct phenotypes, as well as fresh human primary acute and chronic lymphoid leukemia cells; (iv) induced mostly a caspase-independent cell death, which might be an advantage as tumor cells often resist caspase-dependent cell death. Altogether, the present observations suggest that conjugation with plant lectins can allow targeting of photosensitizers towards aberrant glycosylation of tumor cells, e.g. to purge leukemia cells from blood and to preserve the normal leukocytes in extracorporeal photochemotherapy. PMID:21858067

Poiroux, Guillaume; Lafont, Elodie; Ségui, Bruno; Van Damme, Els J. M.; Peumans, Willy J.; Bernadou, Jean; Levade, Thierry; Rougé, Pierre; Barre, Annick; Benoist, Hervé

2011-01-01

285

Effects of pesticides on division of two lepidopteran cell lines and on Autographa Californica MNPV development in TN368 cells  

Microsoft Academic Search

Summary  The concentration of each of 10 pesticides (azinphosmethyl, captan, carbaryl, chlordimedorm, dichlorvos, dimenthoate, fenvalerate,\\u000a methomyl, methyl parathion, trichlorfon) causing a 50% inhibition (ID50) in cell number relative to an untreated culture for a time period equal to four cell doublings was determined for the TN368\\u000a and IPLB-HZ1075 cell lines. The range of ID50 values with either of the cell lines

William J. McCarthy; Tina Hatfield; Susan McMahon

1987-01-01

286

pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen.  

PubMed

In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. These sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated by subcutaneous injection of T13-KD clones showed lower coalescence to fascia and peritoneum, and significantly reduced lung metastasis than control clones. These data suggested that high expression of pp-GalNAc-T13 gene generated trimeric Tn antigen on Syndecan-1, leading to the enhanced metastasis. PMID:22306014

Matsumoto, Yasuyuki; Zhang, Qing; Akita, Kaoru; Nakada, Hiroshi; Hamamura, Kazunori; Tokuda, Noriyo; Tsuchida, Akiko; Matsubara, Takeshi; Hori, Tomoko; Okajima, Tetsuya; Furukawa, Keiko; Urano, Takeshi; Furukawa, Koichi

2012-03-01

287

Loss of catalase activity in Tn1545-induced mutants does not reduce growth of Listeria monocytogenes in vivo.  

PubMed Central

Two catalase-negative mutants of Listeria monocytogenes were obtained by chromosomal insertions of the conjugative transposon Tn1545. The loss of catalase activity did not reduce the level of virulence of these mutants in mice. Indeed, both mutants were capable of growing in host tissues at the same rate as the parental catalase-positive strain. These results favor the view that catalase does not play a critical role in the resistance of L. monocytogenes to macrophage killing. PMID:2545629

Leblond-Francillard, M; Gaillard, J L; Berche, P

1989-01-01

288

Testing and analyses of the TN-24P PWR spent-fuel dry storage cask loaded with consolidated fuel  

SciTech Connect

A performance test of a Transnuclear, Inc. TN-24P storage cask configured for pressurized water reactor (PWR) spent fuel was performed. The work was performed by the Pacific Northwest Laboratory (PNL) and Idaho National Engineering Laboratory (INEL) for the US Department of Energy Office of Civilian Radioactive Waste Management (OCRWM) and the Electric Power Research Institute. The performance test consisted of loading the TN-24P cask with 24 canisters of consolidated PWR spent fuel from Virginia Power's Surry and Florida Power and Light's Turkey Point reactors. Cask surface and fuel canister guide tube temperatures were measured, as were cask surface gamma and neutron dose rates. Testing was performed with vacuum, nitrogen, and helium backfill environments in both vertical and horizontal cask orientations. Transnuclear, Inc., arranged to have a partially insulated run added to the end of the test to simulate impact limiters. Limited spent fuel integrity data were also obtained. From both heat transfer and shielding perspectives, the TN-24P cask with minor refinements can be effectively implemented at reactor sites and central storage facilities for safe storage of unconsolidated and consolidated spent fuel. 35 refs., 93 figs., 17 tabs.

McKinnon, M A; Michener, T E; Jensen, M F; Rodman, G R

1989-02-01

289

Date Name Location July 4 -12 Pueblo County Fair Pueblo  

E-print Network

County Fair Julesburg July 27 - Aug. 2 Las Animas County Fair & Trinidad Round-up Trinidad July 27 - Aug State Fair Pueblo Aug. 28 - 30 Ouray County Fair Ridgway Aug. 30 - Sept. 1 Trinidad Roundup (Las Animas) Trinidad Sept. 3 - 7 Kiowa County Fair & Rodeo Eads Sept. 3 - 7 Jackson County North Park Fair Walden Sept

290

Hudson County Community College Periodic Review Report. Executive Summary.  

ERIC Educational Resources Information Center

Hudson County Community College (HCCC) serves Hudson County, New Jersey. Although the county is the smallest in the state, its 610,000 residents make up one of the most diverse counties in New Jersey. Approximately 40% of residents are Hispanic, 12% are African-American, 10% are Asian, and 35% are White. The county is also home to a growing Middle…

Hudson County Community Coll., Jersey City, NJ.

291

32. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

32. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PROFILE OF HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY IN JOHNSON COUNTY, IOWA, 1897. (RIGHT 1/4 OF DRAWING) PLAN OF APPROACH FOR SUTLIFF'S FERRY BRIDGE OVER CEDAR RIVER, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

292

Waste source reduction county government case study  

SciTech Connect

Itasca County is located in north-central Minnesota, has a population of 42,000 and is known for its forests and scenic waterways. With Beltrami County, it contains the upper watershed of the Mississippi River. Its major industries are timber and tourism. Itasca County government made a commitment to source reduce its waste as much as possible. Secondarily, what they could not reduce they committed themselves to recycle. The project demonstrates functional reduction in practice. It shows that reduction is a realistic goal for county governments and that reduction can be measured on a waste stream by waste stream basis.

NONE

1990-12-31

293

Sonoma County Office of Education Computer Education Plan. County Level Plans.  

ERIC Educational Resources Information Center

This plan describes the educational computing and computer literacy program to be implemented by the schools in Sonoma County, California. Topics covered include the roles, responsibilities, and procedures of the county-wide computer committee; the goals of computer education in the county schools; the results of a needs assessment study; a 3-year…

Malone, Greg

1986-01-01

294

The Economic Impact of Schenectady County Community College on Schenectady County, 1981-82.  

ERIC Educational Resources Information Center

This report on the economic impact of Schenectady County Community College (SCCC) uses a modification of the Caffrey and Isaacs model to assess SCCC-related local business volume, SCCC costs and benefits to the Schenectady County government, and the likely impact on the county if SCCC did not exist. Part I provides background to the study,…

Chestnut, Erma Ruth

295

Interaction of a novel Tn (GalNAc alpha 1-->Ser/Thr) glycoprotein with Gal, GalNAc and GlcNAc specific lectins.  

PubMed

A naturally occurring Tn glycoprotein (Native ASG-Tn) with GalNAc alpha 1-->Ser/Thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. In a quantitative precipitin assay, this glycoprotein completely precipitated Maclura pomifera (MPA), Vicia villosa B4 (VVL-B4) and Artocarpus integrifolia (Jacalin, AIL). It also reacted well with Helix pomatia (HPL) and Wistaria floribunda (WFL) and precipitated over 75% of the lectin nitrogen added, but poorly with Ricinus communis agglutinin (RCA1), ricin, peanut (Arachis hypogaea, PNA), Abrus precatorius agglutinin (APA) and Triticum vulgaris (WGA). This finding suggests that this novel Tn-glycoprotein may serve as a useful reagent for differentiating Tn and T specific monoclonal antibodies and lectins. PMID:8292029

Wu, A M; Wu, J H; Shen, F

1994-01-14

296

Groundwater Transport of Organic Compounds in Old Salvage Yard, Oak Ridge, TN - 12089  

SciTech Connect

In 1950's and early 1960's during production of nuclear weapons at the US Department of Energy Y-12 National Security Complex in Oak Ridge TN, volatile organic compounds (VOCs) as well as heavy metals, nitrates, and radionuclides were released to the environment. Field investigations revealed that much of this contamination is still present in soil, bedrock, and groundwater. Operational buildings and old disposal facilities at the site have been identified as major sources of contamination. The Old Salvage Yard (OSY) on the western side of the site has long been characterized as the major source of VOC contamination in soil and groundwater. In order to analyze the fate and transport of VOC contamination- including tetrachloroethene (PCE), 1,2- dichloroethene (1,2-DCE), cis-1,2-dichloroethene (cis-1,2-DCE) and vinyl chloride (VC) - in groundwater and soil at the vicinity of OSY, an integrated surface and subsurface flow and transport model has been developed for the Y-12 NSC using the hydrodynamic and transport numerical package, MIKE-SHE. Hydrogeological characteristics of the site such as hydraulic conductivity, and transport parameters such as partitioning coefficients were varied in an effort to delineate subsurface flow and transport pathways, potential downstream impacts on Upper East Fork Poplar Creek, and the potential risk to industrial workers involved in related Decontamination and Decommissioning (D and D) activities. The simulation results were compared with the analytical modeling results previously performed by McLane Environmental Inc. using SESOIL-AT123D. Specific simulations have been performed to investigate the effect of possible remedial action (removing the contaminated surface soil layers) on the fate and transport of VOCs. The results of the MIKE-SHE reported here can be considered as an upper limit for the predicted concentrations. Based on MIKE-SHE results, PCE, 1,2 DCE, cis-1,2-DCE, and VC are sources in soil with potential to equal or exceed industrial groundwater hazard and risk levels at the vicinity of OSY. VOC contaminants in soil and groundwater will decay below industrial groundwater risk and hazard levels within approximately 20 years. Excavation of surface soil layers at the site will considerably reduce the concentration of VOCs in groundwater and the possibility of migration of VOCs to surface waters. (authors)

Malek-Mohammadi, Siamak [Civil Engineering and Construction Department, Bradley University, Peoria, IL 61625 (United States); Tachiev, Georgio; Roelant, David [Applied Research Center, Florida International University, Miami, FL 33174 (United States); Bostick, Kent; Daniel, Anamary [Pro2Serve Professional Project Services, Inc., Oak Ridge, TN 37830 (United States)

2012-07-01

297

Geothermal development plan: Pinal county  

SciTech Connect

Wells drilled in the county provide evidence of geothermal energy sufficient for process heat and space heating and cooling applications. Annual energy consumption was estimated for industries whose process heat requirements are less than 105/sup 0/C (221/sup 0/F). This information was then used to model the introduction of geothermal energy into the process heat market. Also, agriculture and agribusiness industries were identified. Many of these are located on or near a geothermal resource and might be able to utilize geothermal energy in their operations.

White, D.H.

1981-01-01

298

Spatial variability assessment of soil nutrients in an intense agricultural area, a case study of Rugao County in Yangtze River Delta Region, China  

NASA Astrophysics Data System (ADS)

Topsoil samples (0-20 cm) ( n = 237) were collected from Rugao County, China. Geostatistical variogram analysis, sequential Gaussian simulation (SGS), and principal component (PC) analysis were applied to assess spatial variability of soil nutrients, identify the possible areas of nutrient deficiency, and explore spatial scale of variability of soil nutrients in the county. High variability of soil nutrient such as soil organic matter (SOM), total nitrogen (TN), available P, K, Fe, Mn, Cu, Zn, and B concentrations were observed. Soil nutrient properties displayed significant differences in their spatial structures, with available Cu having strong spatial dependence, SOM and available P having weak spatial dependence, and other nutrient properties having moderate spatial dependence. The soil nutrient deficiency, defined here as measured nutrient concentrations which do not meet the advisory threshold values specific to the county for dominant crops, namely rice, wheat, and rape seeds, was observed in available K and Zn, and the deficient areas covered 38 and 11%, respectively. The first three PCs of the nine soil nutrient properties explained 62.40% of the total variance. TN and SOM with higher loadings on PC1 are closely related to soil texture derived from different parent materials. The PC2 combined intermediate response variables such as available Zn and P that are likely to be controlled by land use and soil pH. Available B has the highest loading on PC3 and its variability of concentrations may be primarily ascribed to localized anthropogenic influence. The amelioration of soil physical properties (i.e. soil texture) and soil pH may improve the availability of soil nutrients and the sustainability of the agricultural system of Rugao County.

Zhao, Yongcun; Xu, Xianghua; Darilek, Jeremy Landon; Huang, Biao; Sun, Weixia; Shi, Xuezheng

2009-05-01

299

Construction of Mobilizable Mini-Tn7 Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy Promoter lux Reporter Analysis  

PubMed Central

We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria. PMID:23584769

Damron, F. Heath; McKenney, Elizabeth S.; Barbier, Mariette; Liechti, George W.; Schweizer, Herbert P.

2013-01-01

300

Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen  

Microsoft Academic Search

Earlier studies showed thatMoluccella laevis lectin, which has anti-Tn specificity, reacts more strongly with native or desialylated blood group N glycophorin A than with the respective glycophorins of blood group M. We now present results indicating thatVicia villosa B4 anti-Tn lectin, which does not show detectable reaction with untreated glycophorins or erythrocytes, reacts better with desialylated blood group N antigen

Maria Duk; Albert M. Wu; Elwira Lisowska

1994-01-01

301

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.  

PubMed

We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria. PMID:23584769

Damron, F Heath; McKenney, Elizabeth S; Barbier, Mariette; Liechti, George W; Schweizer, Herbert P; Goldberg, Joanna B

2013-07-01

302

77 FR 46682 - Trinity County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Trinity County Resource Advisory Committee AGENCY...SUMMARY: The Trinity County Resource Advisory Committee (RAC...ADDRESSES: The meeting will be held at the Trinity County Office of Education, 201...

2012-08-06

303

76 FR 31297 - Trinity County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Trinity County Resource Advisory Committee AGENCY...SUMMARY: The Trinity County Resource Advisory Committee (RAC...ADDRESSES: The meetings will be held at the Trinity County Office of Education, 201...

2011-05-31

304

76 FR 61379 - Final Recovery Plan, Bexar County Karst Invertebrates  

Federal Register 2010, 2011, 2012, 2013, 2014

...Recovery Plan, Bexar County Karst Invertebrates AGENCY: Fish and Wildlife Service...plan, for the nine Bexar County Karst Invertebrates under the Endangered Species Act of...The following nine Bexar County karst invertebrates were listed as endangered species...

2011-10-04

305

75 FR 31759 - Madera County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...Send written comments to Julie Roberts, Madera County Resource Advisory Committee...

2010-06-04

306

76 FR 12316 - Madera County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

2011-03-07

307

76 FR 19970 - Madera County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...responsibilities for the remainder of the year. The Madera County Resource Advisory Committee...

2011-04-11

308

76 FR 66033 - Madera County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

2011-10-25

309

75 FR 79334 - Madera County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. The Madera...

2010-12-20

310

75 FR 50987 - Madera County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

2010-08-18

311

76 FR 29722 - Madera County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

2011-05-23

312

76 FR 6116 - Madera County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

2011-02-03

313

27 CFR 9.231 - Moon Mountain District Sonoma County.  

Code of Federal Regulations, 2014 CFR

...2014-04-01 2014-04-01 false Moon Mountain District Sonoma County. 9...American Viticultural Areas § 9.231 Moon Mountain District Sonoma County. (a...viticultural area described in this section is “Moon Mountain District Sonoma County”....

2014-04-01

314

Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition  

SciTech Connect

Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZ{alpha}-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cells as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.

Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Matsuura, Masaaki [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University (Japan); Gomi, Yasuyuki [Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University (Japan); Takahashi, Michiaki [Research Foundation for Microbial Diseases of Osaka University (Japan); Yamanishi, Koichi [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.j [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Division of Clinical Virology, Kobe University Graduate School of Medicine (Japan)

2010-06-20

315

A single oligonucleotide can be used to rapidly isolate DNA sequences flanking a transposon Tn5 insertion by the polymerase chain reaction.  

PubMed Central

We have developed a strategy to rapidly construct DNA hybridization probes for the isolation of genes disrupted by transposon Tn5 insertions. A single oligonucleotide complementary to and extending outward from the ends of the inverted repeat of Tn5 was used to prime DNA synthesis in the polymerase chain reaction. The amplified product consisted of DNA sequences adjacent to both ends of the transposon insertion. The general feasibility of the approach was tested by amplifying pBR322 sequences from a derivative of pBR322 containing a Tn5 insertion. To amplify genomic DNA sequences flanking a Tn5 insertion in the chromosome of a Pseudomonas syringae strain, circular substrates were generated by ligating EcoRI-digested genomic DNA. Tn5 was contained intact within one such circular molecule, as the transposon does not contain sites for cleavage by EcoRI. The amplified product (approximately 2.5 kb) was used as a DNA hybridization probe to isolate the homologous fragment from a cosmid library of wild-type Pseudomonas syringae genomic DNA. This approach may be applied to the efficient isolation of sequences flanking any Tn5 insertion. Images PMID:2174544

Rich, J J; Willis, D K

1990-01-01

316

Groundwater management and protection Madison County, Alabama  

SciTech Connect

Groundwater is extremely important to Madison County as it provides nearly three quarters of the county's drinking water. In recent years, Madison County has increasingly recognized the need to protect its groundwater resource. A supply of usable groundwater is one element of a high quality environment, which can help spur economic development and provide for the needs of a growing population. Without planning protection and understanding of possible consequences, however, economic development and population pressures can cause a gradual degradation of groundwater. In April 1987, the Tennessee Valley Authority (TVA) convened a local groundwater steering group in Madison County. At the first meeting the ground agreed upon these goals: (1) to seek incorporate groundwater protection into the planning and development process for Madison County, (2) to support efforts by Madison County to obtain authority to adopt zoning ordinances and subdivision regulations, and (3) to develop a groundwater management plan for the county. This report provides essential information needed in developing a plan and is based on the following assumptions: the citizens of Madison County value the environment in which they live and wish to protect it from pollution; continued economic development is necessary for a healthy local economy; and a healthy economy can be sustained and nurtured, without degradation of the groundwater resource, through countywide planning, education, and participation.

French, J.H.; Strunk, J.W.

1990-07-01

317

Educational and Demographic Profile: Sonoma County.  

ERIC Educational Resources Information Center

This profile uniquely presents a variety of educational and socioeconomic information for Sonoma County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. The information provides a framework for enhanced communication and…

California Postsecondary Education Commission, 2004

2004-01-01

318

Educational and Demographic Profile: Marin County.  

ERIC Educational Resources Information Center

This profile uniquely presents a variety of educational and socioeconomic information for Marin County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced communication…

California Postsecondary Education Commission, 2004

2004-01-01

319

County Data Book 1995: Kentucky Kids Count.  

ERIC Educational Resources Information Center

This data book presents findings of the Kids Count project on current conditions faced by Kentucky children age birth through 19. For each county, and for the state, comparisons are provided between the base years of 1980-1982 and the most recent years 1992-1994. Counties are ranked against each other and trend graphs are provided for the studied…

Kentucky Youth Advocates, Inc., Louisville.

320

Transect Survey Procedures_________________________________________________ COUNTY TRANSECT SURVEY  

E-print Network

soil erosion rates. Chapter 92, Wis. Stats. requires the Land and Water Conservation Board to report University of Wisconsin-Extension Greg Wheeler USDA Natural Resources Conservation Service Dale Hanson Barron Dane County John Pingry USDA Natural Resources Conservation Service Kerri Wheeler Juneau County Kim

Balser, Teri C.

321

Tennessee County Higher Education Profiles, 2010  

ERIC Educational Resources Information Center

The "Tennessee County Higher Education Profiles 2010" provide basic information with respect to higher education for each county in Tennessee. The 2010 "Profiles" represent the fourth year for this report. In the demographic and enrollment sections, a few changes were made to better meet the needs of policymakers. This document explains those…

Tennessee Higher Education Commission, 2010

2010-01-01

322

Socioeconomic Heterogeneity of Mining-Dependent Counties.  

ERIC Educational Resources Information Center

Although the socioeconomic well-being of all U.S. mining-dependent counties was slightly above the national average in 1990, disaggregation reveals substantial effects of region and mining subsector. In particular, southern and Great Lakes coal-mining counties had significantly lower high school graduation rates and higher poverty and unemployment…

Nord, Mark; Luloff, A. E.

1993-01-01

323

COUNTIES OF CALIFORNIA - WITH FARM STATISTICS  

EPA Science Inventory

County farming statistics polygon coverage of California. The statistics were taken from the 1997 Census of Agriculture and added to a coverage extracted from TIGER county boundaries. A farm is any place from which $1,000 or more of agricultural products were sold, or normally w...

324

Educational and Demographic Profile: Trinity County  

ERIC Educational Resources Information Center

This profile uniquely presents a variety of educational and socioeconomic information for Trinity County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

California Postsecondary Education Commission, 2004

2004-01-01

325

Geothermal development issues: Recommendations to Deschutes County  

SciTech Connect

This report discusses processes and issues related to geothermal development. It is intended to inform planners and interested individuals in Deschutes County about geothermal energy, and advise County officials as to steps that can be taken in anticipation of resource development. (ACR)

Gebhard, C.

1982-07-01

326

A Profile of Hardee County, Florida.  

ERIC Educational Resources Information Center

In 1977 leaders of Hardee County, Florida, listed relationships and attitudes of residents, rural atmosphere, environmental conditions, and economic potential among the county's strong points, and public service and facility improvements, developing economic potential, recreational and entertainment development, and planning and zoning as its most…

Beaulieu, Lionel J.; Anderson, Deborah S.

327

The Farm Crisis and Decatur County.  

ERIC Educational Resources Information Center

This case study assesses the impact of the farm sector on the economy and social organization of Decatur County (Kansas), a county which has historically depended on agriculture for its livelihood. Data were obtained from analysis of time series statistical indicators for the period between 1966 and 1984, questionnaire responses of local…

Flora, Jan L.; And Others

328

Transit Rider Information King County Metro Transit  

E-print Network

Transit Rider Information King County Metro Transit Rider Information (206) 553-3000 http://www.kingcounty.gov/metro and after a storm make it easier and safer to clear streets. · Use the bus. SDOT works closely with Metro closely with King County Metro Transit, the Seattle School District, local universities, hospitals

Queitsch, Christine

329

Public Health County of Santa Cruz  

E-print Network

Public Health Division County of Santa Cruz HEALTH SERVICES AGENCY POST OFFICE BOX 962, 1080 at the two Public Health clinics located at 1080 Emeline Ave. in Santa Cruz and at 9 Crestview Dr. in Watsonville, on a walk-in basis. County health clinic services are limited to those who reside in Santa Cruz

California at Santa Cruz, University of

330

Analysis of County School Districts in Arkansas.  

ERIC Educational Resources Information Center

The 1948, Arkansas School District Reorganization Act was passed in an effort to reduce the 1589 small school districts to a smaller number. Those districts not consolidated would form county districts. As of the 1967-68 school year, 26 of these county districts remained. The purpose of this study was to provide information drawing attention to…

Budd, Karol B.; Charlton, J.L.

331

COUNTY BOUNDARIES FOR THE MAIA STUDY REGION  

EPA Science Inventory

This data set is a geographic information system (GIS) coverage of the county boundaries in the United States Environmental Protection Agency (USEPA) Mid-Atlantic Integrated Assessment (MAIA) Project region. The coverage was produced using the US Geological Survey county bound...

332

Alumni by New Jersey County Spring 2011  

E-print Network

Alumni by New Jersey County Spring 2011 COUNTY Atlantic 3,221 Bergen 19,263 Burlington 13,001 - 20,000 Over 20,000 Rutgers, The State University of New Jersey 61 STUDENTS: Alumni #12;Rutgers, The State University of New Jersey 62 STUDENTS: Alumni Alumni by State Spring 2011 Alabama 419 Nebraska 168

Delgado, Mauricio

333

Information Concerning the Washoe County Indian Community.  

ERIC Educational Resources Information Center

Information relative to Indian residents of Washoe County, Nevada, has been developed to provide an analysis of educational and employment needs. Population figures are presented by age and sex for 3 basic Indian residence groups in the county. Statistics are presented in tabular form for Indian student enrollment in area schools, income level of…

Nevada Univ., Reno. Coll. of Education.

334

Educational and Demographic Profile: Madera County  

ERIC Educational Resources Information Center

This profile uniquely presents a variety of educational and socioeconomic information for Madera County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

California Postsecondary Education Commission, 2004

2004-01-01

335

[A Profile of Kershaw County, South Carolina].  

ERIC Educational Resources Information Center

Careful long-range planning and slow, deliberate growth have benefitted Kershaw County's 500,000 pleasantly varied acres near the state capitol. The county, famous for its equestrian activity, boasts prestige stables, riding clubs, and trails. In addition there are lakes and numerous parks. Leaders want to maintain this aesthetic appeal while…

Lilley, Stephen C.; McLean, Edward L.

336

Commodity Flow Study - Clark County, Nevada, USA  

SciTech Connect

The United States Department of Energy has designated Clark County, Nevada as an 'Affected Unit of Local Government' due to the potential for impacts by activities associated with the Yucca Mountain High Level Nuclear Waste Repository project. Urban Transit, LLC has led a project team of transportation including experts from the University of Nevada Las Vegas Transportation Research Center to conduct a hazardous materials community flow study along Clark County's rail and truck corridors. In addition, a critical infrastructure analysis has also been carried out in order to assess the potential impacts of transportation within Clark County of high level nuclear waste and spent nuclear fuel to a proposed repository 90 miles away in an adjacent county on the critical infrastructure in Clark County. These studies were designed to obtain information relating to the transportation, identification and routing of hazardous materials through Clark County. Coordinating with the United States Department of Energy, the U.S. Department of Agriculture, the U. S. Federal Highway Administration, the Nevada Department of Transportation, and various other stakeholders, these studies and future research will examine the risk factors along the entire transportation corridor within Clark County and provide a context for understanding the additional vulnerability associated with shipping spent fuel through Clark County. (authors)

Conway, S.Ph.D. [Urban Environmental Research LLC, Las Vegas, NV (United States); Navis, I. [AICP Planning Manager, Clark County Nuclear Waste Division, Department of Comprehensive Planning, Las Vegas, NV (United States)

2008-07-01

337

Youth Political Culture in a Kentucky County.  

ERIC Educational Resources Information Center

Data from a self-administered questionnaire survey of all 188 high school juniors and seniors who attended the only high school in a rural county in Kentucky were utilized to explore various dimensions of youth political culture in that county. The study was part of a continuing effort to understand and analyze the political culture and…

Mohapatra, Manindra Kumar

338

Pennsylvania Dutch Country of Lancaster County  

NSDL National Science Digital Library

The Pennsylvania Dutch Convention And Visitors Bureau, in cooperation with the attractions of the Amish Country of Lancaster County, Pennsylvania, now offer a complete virtual information center on the Pennsylvania Dutch Country of Lancaster County. Includes information on the Amish, quilts, covered bridges, and all the interesting things to see and do in the area.

339

76 FR 19030 - Lyon & Mineral County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service Lyon & Mineral County Resource Advisory Committee AGENCY: Forest Service...SUMMARY: The Lyon and Mineral County Resource Advisory Committee will meet in Smith...

2011-04-06

340

Shoreham Railroad Bridge, Former Addison County Railroad (later, Rutland Railroad, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Shoreham Railroad Bridge, Former Addison County Railroad (later, Rutland Railroad, Addison Branch), spanning Lemon Fair River above Richville Pond, west of East Shoreham Road, Shoreham, Addison County, VT

341

7. Photocopy of photograph (from Broome County Historical Society) showing ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photocopy of photograph (from Broome County Historical Society) showing SWIMMERS, PHOTOGRAPH TAKEN FACING NORTHEAST - Charles F. Johnson Pool, Charles F. Johnson Park, Johnson City, Broome County, NY

342

75 FR 18877 - Notice of Invitation to Participate; Exploration for Coal in Utah License Application UTU-87041  

Federal Register 2010, 2011, 2012, 2013, 2014

...to Participate; Exploration for Coal in Utah License Application UTU-87041 AGENCY...located in Emery and Sevier Counties, Utah. DATES: Any party electing to participate...weeks in the Richfield Reaper, Richfield, Utah. ADDRESSES: The exploration plan,...

2010-04-13

343

Imperial County geothermal development annual meeting: summary  

SciTech Connect

All phases of current geothermal development in Imperial County are discussed and future plans for development are reviewed. Topics covered include: Heber status update, Heber binary project, direct geothermal use for high-fructose corn sweetener production, update on county planning activities, Brawley and Salton Sea facility status, status of Imperial County projects, status of South Brawley Prospect 1983, Niland geothermal energy program, recent and pending changes in federal procedures/organizations, plant indicators of geothermal fluid on East Mesa, state lands activities in Imperial County, environmental interests in Imperial County, offshore exploration, strategic metals in geothermal fluids rebuilding of East Mesa Power Plant, direct use geothermal potential for Calipatria industrial Park, the Audubon Society case, status report of the Cerro Prieto geothermal field, East Brawley Prospect, and precision gravity survey at Heber and Cerro Prieto geothermal fields. (MHR)

Not Available

1983-01-01

344

Environmental assessment: Deaf Smith County site, Texas  

SciTech Connect

In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and the eight other potentially sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines. On the basis of these findings, the DOE is nominating the Deaf Smith County site as one of the five sites suitable for characterization. 591 refs., 147 figs., 173 tabs.

Not Available

1986-05-01

345

Fall 2010 Davis County Tuesday Workshop  

NSDL National Science Digital Library

Participant materials for those participating in the Tuesday Fall, 2010 Davis County IA Workshop . For viewing and printing convenience each resource is a pdf. For your convenience, there is a link to download the free Adobe Reader. Download Adobe Reader Workshop Materials Handouts Day 1 Workshop Handout and Materials Davis County Tuesday Day 2 Handouts Davis County Tuesday Day 3 Handouts Reflection Paper Prompts Fall 2010 Davis County Workshops First Reflection Prompts Fall 2010 Davis County Workshops Second Reflection Prompts Sample Instructional Architect Projects Solving One Step equations: Pre-Algebra Standard 3 Objective 2: Students will solve one step equations. The students will use addition and subtraction to solve the equations. Cells: Students will have the opportunity to explore the cell in a 3D fashion and learn more ...

Brooke Robertshaw

2010-09-22

346

Intra- and Interspecies Conjugal Transfer of Tn916-Like Elements from Lactococcus lactis In Vitro and In Vivo?  

PubMed Central

Tetracycline-resistant Lactococcus lactis strains originally isolated from Polish raw milk were analyzed for the ability to transfer their antibiotic resistance genes in vitro, using filter mating experiments, and in vivo, using germfree rats. Four of six analyzed L. lactis isolates were able to transfer tetracycline resistance determinants in vitro to L. lactis Bu2-60, at frequencies ranging from 10?5 to 10?7 transconjugants per recipient. Three of these four strains could also transfer resistance in vitro to Enterococcus faecalis JH2-2, whereas no transfer to Bacillus subtilis YBE01, Pseudomonas putida KT2442, Agrobacterium tumefaciens UBAPF2, or Escherichia coli JE2571 was observed. Rats were initially inoculated with the recipient E. faecalis strain JH2-2, and after a week, the L. lactis IBB477 and IBB487 donor strains were introduced. The first transconjugants were detected in fecal samples 3 days after introduction of the donors. A subtherapeutic concentration of tetracycline did not have any significant effect on the number of transconjugants, but transconjugants were observed earlier in animals dosed with this antibiotic. Molecular analysis of in vivo transconjugants containing the tet(M) gene showed that this gene was identical to tet(M) localized on the conjugative transposon Tn916. Primer-specific PCR confirmed that the Tn916 transposon was complete in all analyzed transconjugants and donors. This is the first study showing in vivo transfer of a Tn916-like antibiotic resistance transposon from L. lactis to E. faecalis. These data suggest that in certain cases food lactococci might be involved in the spread of antibiotic resistance genes to other lactic acid bacteria. PMID:19666731

Boguslawska, Joanna; Zycka-Krzesinska, Joanna; Wilcks, Andrea; Bardowski, Jacek

2009-01-01

347

Knox County Black History Archives  

NSDL National Science Digital Library

Knox County, Ohio has had a significant black population for several hundred years, and historians and others have documented their activities in journal articles, books, and other printed materials. This remarkable digital collection from the Digital Resource Commons at Kenyon College provides access to photographs, documents, and other items that tell the story of this unique community. This collection was made possible, in part, by funding from the Ohio Humanities Council, along with support from the Rural Life Center. Currently the collection includes 200 items, which visitors can browse alphabetically or by performing a keyword search. Each item has a preview feature and visitors can click through to zoom in on each item. The handwritten history of Mt. Cavalry Baptist Church is particularly fascinating.

2012-06-01

348

Costilla County Biodiesel Pilot Project  

SciTech Connect

The Costilla County Biodiesel Pilot Project has demonstrated the compatibility of biodiesel technology and economics on a local scale. The project has been committed to making homegrown biodiesel a viable form of community economic development. The project has benefited by reducing risks by building the facility gradually and avoiding large initial outlays of money for facilities and technologies. A primary advantage of this type of community-scale biodiesel production is that it allows for a relatively independent, local solution to fuel production. Successfully using locally sourced feedstocks and putting the fuel into local use emphasizes the feasibility of different business models under the biodiesel tent and that there is more than just a one size fits all template for successful biodiesel production.

Doon, Ben; Quintana, Dan

2011-08-25

349

Operational assessment of the transnuclear TN-9 truck spent fuel shipping cask: studies and research concerning BNFP  

SciTech Connect

This report presents the results of an operational assessment of the Transnuclear Inc., TN-9 spent fuel cask. This packaging system transports seven current generation boiling-water-reactor nuclear fuel assemblies in a truck shipping mode. The studies were performed at the Barnwell Nuclear Fuel Plant by employees of Allied-General Nuclear Services. The work was funded by the Department of Energy during fiscal year 1981. The cooperation of Transnuclear in this effort is gratefully acknowledged. The study is based on repeated simulated unloading runs of TN-9. Specific tasks and areas of study included: (1) sequential dry-run handling operations under simulated unloading conditions, (2) detailed time and manpower studies, (3) estimates of operator radiation exposure, (4) a general evaluation of the cask system capabilities as they relate to unloading and loading facility operations, and (5) preparation of operating procedures for both unloading (confirmed by practice runs) and loading (yet to be confirmed). Also included is general information on the cask, auxiliary equipment, and the Certificate of Compliance.

McCreery, P.N.

1981-11-01

350

Improved Glycosylation of a Foreign Protein by Tn-5B1-4 Cells Engineered to Express Mammalian Glycosyltransferases  

PubMed Central

The major advantages of using the baculovirus–insect cell system for recombinant protein production are its ability to produce large amounts of recombinant proteins and its ability to provide eucaryotic modifications, such as glycosylation. However, the glycans linked to recombinant glycoproteins produced by this system typically differ from those found on native mammalian products. This is an important problem because glycans on mammalian glycoproteins can influence their functions in many different ways. The inability of baculovirus-infected insect cells to produce glycans identical to those found on native mammalian glycoproteins is due, in part, to the absence of functional levels of certain glycosyltransferases in insect cells. Thus, the purpose of this study was to engineer these activities into Tn-5B1-4, an established insect cell line that is widely used as a host for baculovirus-mediated protein production. Expression plasmids were constructed in which cDNAs encoding mammalian ?1,4-galactosyltransferase and ?2,6-sialyltransferase were placed under the transcriptional control of a baculovirus immediate early promoter. These plasmids were then used to isolate two different transgenic Tn-5B1-4 derivatives and the biological and biochemical properties of these cell lines were examined. The results show that both of the engineered insect cell lines have improved glycoprotein-processing capabilities, relative to the parental cell line. PMID:11400096

Breitbach, Kevin

2013-01-01

351

Interaction of the Tn7-encoded transposition protein TnsB with the ends of the transposon.  

PubMed Central

We have used several high resolution methods to examine the interaction of TnsB, a transposition protein encoded by the bacterial transposon Tn7, with its binding sites at the ends of the transposon. These binding sites lie within the DNA segments that are directly involved in transposition. We show that the binding of TnsB to DNA can promote DNA bending, suggesting that the interaction of TnsB with the ends may result in formation of a highly organized protein-DNA complex. We also identify likely positions of close contact between of TnsB and its binding sites. Analysis of the interaction of TnsB with intact Tn7 ends reveals TnsB occupies its binding sites in a particular order, the sites immediately adjacent to the transposon termini being occupied only after other inner sites are bound. Such ordered occupancy suggests that the various binding sites have differing apparent affinities for TnsB. Images PMID:1656385

Arciszewska, L K; Craig, N L

1991-01-01

352

Tri-county pilot study. [Texas  

NASA Technical Reports Server (NTRS)

The author has identified the following significant results. An area inventory was performed for three southeast Texas counties (Montgomery, Walker, and San Jacinto) totaling 0.65 million hectares. The inventory was performed using a two level hierarchy. Level 1 was divided into forestland, rangeland, and other land. Forestland was separated into Level 2 categories: pine, hardwood, and mixed; rangeland was not separated further. Results consisted of area statistics for each county and for the entire study site for pine, hardwood, mixed, rangeland, and other land. Color coded county classification maps were produced for the May data set, and procedures were developed and tested.

Reeves, C. A. (principal investigator); Austin, T. W.; Kerber, A. G.

1976-01-01

353

Evaluation of the Union County Alternative to Suspension Program  

ERIC Educational Resources Information Center

The schools in Union County have undergone a tremendous amount of growth in the past decade. The growth in the county has led to an increase in discipline problems. In order to provide suspended students a second chance, Union County Public Schools implemented an alternative to suspension program, the Union County Alternative to Suspension Program…

Dunlap, Joyce Ann

2010-01-01

354

34. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

34. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PLAN OF RIVER PIERS FOR HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

355

31. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PROFILE OF HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY IN JOHNSON COUNTY, IOWA, 1897. (LEFT 3/4 OF DRAWING) - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

356

NRCS Nutrient Management Field Team Coordinators Counties in Field Office  

E-print Network

Gary.Swope@pa.usda.gov Allegheny County W4 Butler Kittanning Laurel Hoffman Laurel.Hoffman@pa.usda.gov Armstrong County W4 Kittanning Kittanning Laurel Hoffman Laurel.Hoffman@pa.usda.gov Butler County W4 Butler Kittanning Laurel Hoffman Laurel.Hoffman@pa.usda.gov Beaver County W4 Butler Kittanning Laurel Hoffman Laurel.Hoffman

Guiltinan, Mark

357

The biphenyl- and 4-chlorobiphenyl-catabolic transposon Tn4371, a member of a new family of genomic islands related to IncP and Ti plasmids.  

PubMed

The nucleotide sequence of the biphenyl catabolic transposon Tn4371 has been completed and analyzed. It confirmed that the element has a mosaic structure made of several building blocks. In addition to previously identified genes coding for a tyrosine recombinase related to phage integrases and for biphenyl degradation enzymes very similar to those of Achromobacter georgiopolitanum KKS102, Tn4371 carries many plasmid-related genes involved in replication, partition, and other, as-yet-unknown, plasmid functions. One gene cluster contains most of the genes required to express a type IV secretion-mating pair formation apparatus coupled with a TraG ATPase, all of which are related to those found on IncP and Ti plasmids. Orthologues of all Tn4371 plasmid-related genes and of the tyrosine recombinase gene were found, with a very similar organization, in the chromosome of Ralstonia solanacearum and on the yet-to-be-determined genomic sequences of Erwinia chrysanthemi and Azotobacter vinelandii. In each of these chromosomal segments, conserved segments were separated by different groups of genes, which also differed from the Tn4371 bph genes. The conserved blocks of genes were also identified, in at least two copies, in the chromosome of Ralstonia metallidurans CH34. Tn4371 thus appears to represent a new family of potentially mobile genomic islands with a broad host range since they reside in a wide range of soil proteobacteria, including plant pathogens. PMID:12902278

Toussaint, Ariane; Merlin, Christophe; Monchy, Sébastien; Benotmane, M Abderrafi; Leplae, Raphaël; Mergeay, Max; Springael, Dirk

2003-08-01

358

Analysis of the temporal expression of Trichoplusia ni single nucleopolyhedrovirus genes following transfection of BT1-Tn-5B1-4 cells.  

PubMed

Trichoplusia ni single nucleopolyhedrovirus (TnSNPV), a 134,394-bp double-stranded DNA group II Nucleopolyhedrovirus, is pathogenic to the lepidopteran T. ni. TnSNPV transcription is temporally regulated and divided into three promoter sequence-dependent classes (early, late and very late genes). A viral oligonucleotide DNA microarray containing all potential (144) viral genes of TnSNPV was designed to investigate global viral gene expression during cell infection. Total BT1-Tn-5B1-4 cellular mRNAs extracted between 0 and 72 h posttransfection with TnSNPV genomic DNA were hybridized to the microarray. Initial average expression of early genes was detected between 12 and 24 h posttransfection while late genes were mainly detected between 24 and 72 h posttransfection. The microarray expression profiling data verified many computer predicted promoter assignments. K-means clustering was used to sort the 144 genes based on their temporal expression pattern similarities. This clustering resulted in the confirmation and temporal class assignment of previously unidentified genes and promoters. PMID:16872655

van Munster, Manuela; Willis, Leslie G; Elias, Miria; Erlandson, Martin A; Brousseau, Roland; Theilmann, David A; Masson, Luke

2006-10-10

359

Analysis of the Shallow Groundwater Flow System at Fire Island National Seashore, Suffolk County, New York  

USGS Publications Warehouse

Fire Island National Seashore (FIIS) occupies 42 kilometers of the barrier island for which it is named that lies off the southern shore of Suffolk County, N.Y. Freshwater in the highly permeable, sandy aquifer underlying Fire Island is bounded laterally by marine surface waters and at depth by saline groundwater. Interspersed throughout FIIS are 17 pre-existing residential communities that in summer months greatly increase in population through the arrival of summer residents and vacationers; in addition, the National Park Service (NPS) has established several facilities on the island to accommodate visitors to FIIS. The 2.2 million people estimated by the NPS to visit Fire Island annually impact groundwater quality through the release of waste-derived contaminants, such as nutrients, pathogens, and organic compounds, into the environment. Waste-contaminated groundwater can move through the aquifer and threaten the ecological health of the adjacent back-barrier estuaries to which much of the groundwater ultimately discharges. In 2004, the U.S. Geological Survey (USGS), in cooperation with the NPS, began a 3-year investigation to (1) collect groundwater levels and water-quality (nutrient) samples, (2) develop a three-dimensional model of the shallow (water-table) aquifer system and adjacent marine surface waters, and (3) calculate nitrogen loads in simulated groundwater discharges from the aquifer to back-barrier estuaries and the ocean. The hydrogeology of the shallow aquifer system was characterized from the results of exploratory drilling, geophysical surveying, water-level monitoring, and water-quality sampling. The investigation focused on four areas-the communities of Kismet and Robbins Rest, the NPS Visitor Center at Watch Hill, and the undeveloped Otis Pike Fire Island High Dune Wilderness. Thirty-five observation wells were installed within FIIS to characterize subsurface hydrogeology and establish a water-table monitoring network in the four study areas. A variable-density model of the shallow aquifer system and adjacent marine surface waters was developed to simulate groundwater flow patterns and rates. Nitrogen loads from the shallow aquifer system were calculated from representative total nitrogen (TN) concentrations and simulated groundwater discharges to back-barrier estuaries and the ocean. The model simulates groundwater directions, velocities, and discharge rates under 2005 mean annual conditions. Groundwater budgets were developed for recharge areas of similar land use that contribute freshwater to back-barrier estuaries, the ocean, and subsea-discharge zones. Total freshwater discharge from the shallow aquifer system is about 43,500 cubic meters per day (m3/d) (79.8 percent) to back-barrier estuaries and about 10,200 m3/d (18.7 percent) to the ocean; about 836 m3/d (1.5 percent) may exit the system as subsea underflow. The total contribution of fresh groundwater to shoreline discharge zones amounts to about 53,700 m3/d (98.5 percent). The median age of freshwater discharged to back-barrier estuaries and the ocean was 3.4 years, and the 95th-percentile age was 20 years. The TN concentrations and loads under 2005 mean annual conditions for areas that contribute fresh groundwater to back-barrier estuaries and the ocean were calculated for the principal land uses on Fire Island. The overall TN load from the shallow aquifer system to shoreline discharge zones is about 16,200 kilograms per year (kg/yr) (82.2 percent) to back-barrier estuaries and about 3,500 kg/yr (17.8 percent) to the ocean. The overall TN load to marine surface waters amounts to about 19,700 kg/yr-roughly 6 percent of the annual TN load from shallow groundwater entering the South Shore Estuary Reserve (SSER) from the Suffolk County mainland, which is about 345,000 kg/yr. In contrast to the TN load from shallow groundwater for the SSER watershed, which annually yields about 353 kilograms per square kilometer (kg/km2), the overall TN loa

Schubert, Christopher E.

2010-01-01

360

2013 Gloucester County 4-H Fair  

E-print Network

Cooperative Extension of Gloucester County 1200 N. Delsea Drive Building A Clayton, NJ 08312 Phone: 856 professionals arrive. For example: Bandages Vet wrap Anti-bacterial soap Scissors Medical tape Betadine/ iodine

Goodman, Robert M.

361

Environmental assessment: Deaf Smith County site, Texas  

SciTech Connect

In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of the nine potentially acceptable sites for mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines.

Not Available

1986-05-01

362

Environmental assessment, Deaf Smith County site, Texas  

SciTech Connect

The Nuclear Waste Policy Act of 1982 (42 USC sections 10101-10226) requires the environmental assessment of a proposed site to include a statement of the basis for nominating a site as suitable for characterization. Volume 2 provides a detailed statement evaluating the site suitability of the Deaf Smith County Site under DOE siting guidelines, as well as a comparison of the Deaf Smith County Site to the other sites under consideration. The evaluation of the Deaf Smith County Site is based on the impacts associated with the reference repository design, but the evaluation will not change if based on the Mission Plan repository concept. The second part of this document compares the Deaf Smith County Site to Davis Canyon, Hanford, Richton Dome and Yucca Mountain. This comparison is required under DOE guidelines and is not intended to directly support subsequent recommendation of three sites for characterization as candidate sites. 259 refs., 29 figs., 66 refs. (MHB)

Not Available

1986-05-01

363

The Medina County, Ohio, central processing facility  

SciTech Connect

Before deciding on the appropriate county recycling facility for their communities, waste management officials from Medina County, Ohio, spent three years researching their options. First and foremost, they wanted to generate a recycling amount that would at least double House Bill 592's requirements which calls for a 25% reduction in reliance on landfill space. After issuing a request for proposals, the county opted for a central processing facility (CPF)--which was designed, built, and is now operated by Norton Environmental (Independence, Ohio). Currently, the CPF extracts and processes recyclables, materials for refuse-derived fuel (RDF), and compostables from a mixed solid waste stream. After operating for just over one year, the facility, which is located in Seville, Ohio, is well on its way to achieving its goal of recovering two-thirds of the county's incoming mixed solid waste stream, and waste management officials there couldn't be happier with their selection.

White, K.M.

1994-08-01

364

PENNSYLVANIA GEOGRAPHIC INFORMATION SYSTEM LIBRARY: COUNTY BOUNDARIES  

EPA Science Inventory

Pennsylvania Geographic Information System (GIS) Library provides a data set of complete digitized county boundaries of the Commonwealth Of Pennsylvania at a scale of 1:24,000 from USGS topographic maps. The data format is ARC/INFO Export....

365

CHarlotteCounty Number of volunteers 339  

E-print Network

$183,585 CharlotteCooperativeExtensionService 25550 Harbor View Road, Ste 3 Port Charlotte, FL 33980-Friendly LandscapingTM, Waterway Management, Commercial Horticulture Giving Recent donors residing in county 1,040 FY

Jawitz, James W.

366

CHarlotteCounty Number of volunteers 375  

E-print Network

worked $312,098 CharlotteCo.CooperativeExtensionService 25550 Harbor View Road, Suite 3 Port Charlotte-Friendly LandscapingTM, Waterway Management, Commercial Horticulture Giving Recent donors residing in county 1,021 FY

Watson, Craig A.

367

69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C.L. Wood, the county engineer. Updated through the mid-1930s to show new federal aid-state roads. Compares modern system with older county system. Original scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

368

SOLAR PANELS ON HUDSON COUNTY FACILITIES  

SciTech Connect

This project involved the installation of an 83 kW grid-connected photovoltaic system tied into the energy management system of Hudson County's new 60,000 square foot Emergency Operations and Command Center and staff offices. Other renewable energy features of the building include a 15 kW wind turbine, geothermal heating and cooling, natural daylighting, natural ventilation, gray water plumbing system and a green roof. The County intends to seek Silver LEED certification for the facility.

BARRY, KEVIN

2014-06-06

369

County Extension Home Economics Program: Organizational Relationships.  

E-print Network

plan. Committee members also assist in deter- mining the dectiveness of the in-depth educational p S. The program chairmen (vice presidents) of the county Entensh homemakers council and the Texas Extension Homemakers hpsmiath represent the Extension... homemakers cound. &mion Homemakers C l u b Extension homemakers clubs are community groups of homemak- e& who work with the county Extension aged to plan and carry out educational programs in home economics. - Exbrision homemakers clubs participate...

Cline, June

1982-01-01

370

Edwards Aquifer Evaluation: Kinney County, Texas  

NASA Astrophysics Data System (ADS)

The Edwards Aquifer is one of the most studied and most prolific aquifers in the United States. The aquifer is a heavily fractured and faulted carbonate aquifer with transmissivities in excess of 100 ft2/s. The City of San Antonio relies upon the Edwards Aquifer as its sole source for water. Much work has been done on quantifying recharge to the aquifer and discharge from wells and acquiring aquifer characteristics from pumping tests, specific capacity tests, and geophysical logs. Although the aquifer has been well studied in Bexar County, much less is known about the Edwards Aquifer in Kinney County. This is partly due to the lower population within the county (approximately 3,500 people) relative to the eastern counties (Uvalde, Medina, Bexar, Comal, and Hays) and the great distance of Kinney County from high profile discharge areas such as the City of San Antonio and Comal and San Marcos Springs. Three key products resulted from this study: (1) exploratory well drilling and the largest aquifer test in the county that were conducted to evaluate the well yields within a 10,000 acre study area in which a drawdown of 2.5 ft approximately 1.2 miles away was observed while pumping at approximately 4,600 gpm; (2) a recharge estimate for the Edwards Aquifer within Kinney County of approximately 71,382 ac-ft/yr; and (3) locating the Brackettville Groundwater Divide from an evaluation of ground water flow direction and hydrograph analysis. These results help evaluate the complex hydraulics occurring within Kinney County and aid in development of ground water modeling that will be used in managing the Edwards Aquifer.

Khorzad, Kaveh

2003-10-01

371

The Water Cycle in Volusia County  

USGS Publications Warehouse

Earth's water is always in motion. The water cycle, also known as the hydrologic cycle, describes the continuous movement of water on, above, and below the Earth's surface. This fact sheet provides information about how much water moves into and out of Volusia County, and where it is stored. It also illustrates the seasonal variation in water quantity and movement using data from some of the hydrologic data collection sites in or near Volusia County, Florida.

German, Edward R.

2009-01-01

372

Tiu Ch Chn La D n Khi Phc Sm Vo ngy 21 thng 4 nm 2011, cc y Vin nh Gi Tn Hi  

E-print Network

Tiêu Chí Chn La D Án Khôi Phc Sm Vào ngày 21 tháng 4 nm 2011, các y Viên ánh Giá Tn Hi Tài Nguyên các y viên trong vic tr li nh ban u các tài nguyên thiên nhiên ã b tn hi và/hoc n bù các thit hi tm s tn hi cng là mt yu t quan trng trong quy trình la chn d án); Kh nng thành công ca tng gii pháp thay

373

Geothermal development plan: Maricopa County  

SciTech Connect

The Maricopa County Geothermal Development Plan evaluated the market potential for utilizing geothermal energy. The study identified six potential geothermal resource areas with temperatures less than 100{sup 0}C (212{sup 0}F) and in addition, four suspected intermediate temperature areas (90{sup 0} to 150{sup 0}C, 194{sup 0} to 300{sup 0}F). Geothermal resources are found to occur in and near the Phoenix metropolitan area where average population growth rates of two to three percent per year are expected over the next 40 years. Rapid growth in the manufacturing, trade and service sectors of the regional economy provides opportunities for the direct utilization of geothermal energy. A regional energy use analysis is included containing energy use and price projections. Water supplies are found to be adequate to support this growth, though agricultural water use is expected to diminish. The study also contains a detailed section matching geothermal resources to potential users. Two comparative analyses providing economic details for space heating projects are incorporated.

White, D.H.; Goldstone, L.A.

1982-08-01

374

Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system.  

PubMed Central

The human alpha-3/4-fucosyltransferase III (Fuc-TIII) participates in the synthesis of Lewis determinants. The enzyme from human sources is scarce and heterogeneous. In this paper we describe the expression of a secreted form of Fuc-TIII (SFT3) in two insect cell lines, Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn), using the baculovirus expression system. The Sf9 cells secreted approx. 0.4 unit/l (1 mg/l) of the enzyme. The Tn cells secreted approx. 3-fold this amount. A large proportion of active protein was accumulated in the two cell lines (50 and 75% respectively for Sf9 and Tn cells, on the fourth day after infection) indicating a possible limitation not only of the folding machinery, but also a saturation of the secretory pathway. SFT3 was purified by cation-exchange chromatography followed by affinity chromatography. The enzyme from the Tn cell line had a lower global charge, possibly due to post-translational modifications, such as phosphorylation or sulphation. The two glycosylation sites from SFT3 were occupied. SFT3 secreted by Sf9 cells was completely deglycosylated by peptide-N-glycanase F, whereas 50% of SFT3 secreted by Tn cells was resistant to deglycosylation by this enzyme. The apparent kinetic parameters determined with the type I acceptor were k(cat)=0.4 s(-1) and K(m)=0.87 mM for the SFT3 secreted by Tn cells, and k(cat)=0.09 s(-1) and K(m)=0.76 mM for the SFT3 secreted by Sf9 cells, indicating that the enzymes had substrate affinities within the same order of magnitude as their mammalian counterpart. Furthermore, SFT3 secreted by either cell type showed a clear preference for type 1 carbohydrate acceptors, similarly to human Fuc-TIII. PMID:11171070

Morais, V A; Serpa, J; Palma, A S; Costa, T; Maranga, L; Costa, J

2001-01-01

375

IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China.  

PubMed

Achromobacter xylosoxidans strain A22732 is isolated from a pneumonia patient in China and produces carbapenemases OXA-114e and IMP-1, which are encoded by chromosome and plasmid, respectively, and confer resistance to multiple ß-lactam antibiotics including carbapenems. The blaIMP-1 gene together with aacA7 and orfE is captured by a novel Tn402-like class 1 integron in a conjugative IncP-1ß plasmid. In addition to the intrinsic integron promoter PcW, there is still a blaIMP-1 gene cassette-specific promoter. This is the first report of carbapenemase-encoding IncP-1ß plasmid in clinical bacterial isolate. PMID:25428613

Chen, Zhenhong; Fang, Haihong; Wang, Li; Sun, Fengjun; Wang, Yong; Yin, Zhe; Yang, Huiying; Yang, Wenhui; Wang, Jie; Xia, Peiyuan; Zhou, Dongsheng; Liu, Changting

2014-01-01

376

Annual Performance Evaluation of a Pair of Energy Efficient Houses (WC3 and WC4) in Oak Ridge, TN  

SciTech Connect

Beginning in 2008, two pairs of energy-saver houses were built at Wolf Creek in Oak Ridge, TN. These houses were designed to maximize energy efficiency using new ultra-high-efficiency components emerging from ORNL s Cooperative Research and Development Agreement (CRADA) partners and others. The first two houses contained 3713 square feet of conditioned area and were designated as WC1 and WC2; the second pair consisted of 2721 square feet conditioned area with crawlspace foundation and they re called WC3 and WC4. This report is focused on the annual energy performance of WC3 and WC4, and how they compare against a previously benchmarked maximum energy efficient house of a similar footprint. WC3 and WC4 are both about 55-60% more efficient than traditional new construction. Each house showcases a different envelope system: WC3 is built with advanced framing featured cellulose insulation partially mixed with phase change materials (PCM); and WC4 house has cladding composed of an exterior insulation and finish system (EIFS). The previously benchmarked house was one of three built at the Campbell Creek subdivision in Knoxville, TN. This house (CC3) was designed as a transformation of a builder house (CC1) with the most advanced energy-efficiency features, including solar electricity and hot water, which market conditions are likely to permit within the 2012 2015 period. The builder house itself was representative of a standard, IECC 2006 code-certified, all-electric house built by the builder to sell around 2005 2008.

Biswas, Kaushik [ORNL; Christian, Jeffrey E [ORNL; Gehl, Anthony C [ORNL; Jackson, Roderick K [ORNL; Boudreaux, Philip R [ORNL

2012-04-01

377

76 FR 30025 - Revisions to the California State Implementation Plan, Placer County Air Pollution Control...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Implementation Plan, Placer County Air Pollution Control District and Ventura County Air Pollution Control District AGENCY: Environmental...revisions to the Placer County Air Pollution Control District (PCAPCD)...

2011-05-24

378

76 FR 30080 - Revisions to the California State Implementation Plan, Placer County Air Pollution Control...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Implementation Plan, Placer County Air Pollution Control District and Ventura County Air Pollution Control District AGENCY: Environmental...revisions to the Placer County Air Pollution Control District (PCAPCD)...

2011-05-24

379

How Are Kentucky's Children Stacking Up? A County by County Analysis.  

ERIC Educational Resources Information Center

In a county by county analysis, this report assesses the quality of life for Kentucky's children. Researchers developed a child quotient (CQ) based on 18 indicators: per capita income, children in poverty, women receiving inadequate prenatal care, infant deaths, teens giving birth, substandard dwellings, children in foster care, per-pupil…

Chandler, Betsy

380

Public Health Services in Sixty-Seven Rural Counties: Are They Meeting the County's Medical Needs?  

ERIC Educational Resources Information Center

Forty-four counties in Florida and 23 in Oregon with 1966 populations of less than 50,000 were selected for a study of the adequacy of public health services in rural areas. The counties were ranked on the following need variables: number of children under 5 years, persons over 65 years, Negroes, and families with incomes under $3,000. The seven…

Turk, William L.

381

Citrus County Schools Copyright Guidelines Recommended by the Citrus County Association of School Media Specialists.  

ERIC Educational Resources Information Center

This document contains copyright guidelines determined appropriate for the Citrus County School System (Florida) by the Citrus County Association of School Media Specialists in May, 1992. These guidelines are based on interpretation and understanding of current copyright law as applied to education and implemented in school districts in the United…

Citrus County School District, Inverness, FL.

382

76 FR 4254 - Irish Potatoes Grown in Certain Designated Counties in Idaho, and Malheur County, Oregon...  

Federal Register 2010, 2011, 2012, 2013, 2014

...AMS-FV-10-0109; FV11-945-1] Irish Potatoes Grown in Certain Designated Counties...conducted among eligible producers of Irish potatoes in certain designated counties in Idaho...order regulating the handling of Irish potatoes grown in the production area....

2011-01-25

383

Analysis of common IL-6 promoter SNP variants and the AnTn tract in humans and primates and effects on plasma IL-6 levels  

E-print Network

Analysis of common IL-6 promoter SNP variants and the AnTn tract in humans and primates and effects in the promoter of IL-6. Analysis in five different primate species demonstrated a G allele at Ã? 597, Ã? 572 and Ã?

Rockman, Matthew

384

The Funk Transform as a Penrose Transform T.N. Bailey M.G. Eastwood A.R. Gover L.J. Mason  

E-print Network

The Funk Transform as a Penrose Transform T.N. Bailey M.G. Eastwood A.R. Gover L.J. Mason December 2, 1999 Abstract The Funk transform is the integral transform from the space of smooth even this transform as a limit in a certain sense of the Penrose transform from C P 2 to C P \\Lambda 2 . We exploit

Gover, Rod

385

Staff Member, Staff Member, and Staff Supervisor, respectively, Oak Ridge National Laboratory, Engineering Technology Division, Oak Ridge, TN 37831-8066.  

E-print Network

1 Staff Member, Staff Member, and Staff Supervisor, respectively, Oak Ridge National Laboratory Conshohocken, PA, 2002. Abstract Oak Ridge National Laboratory has developed a new technique to monitor, Engineering Technology Division, Oak Ridge, TN 37831-8066. D. E. Welch1 , L. M. Hively1 , R. F. Holdaway1 STP

Hively, Lee M.

386

Directing enhancer-traps and iTol2 end-sequences to deleted BAC ends with loxP- and lox511-Tn10 transposons.  

PubMed

A step-by-step detailed procedure is presented to progressively truncate genomic DNA inserts from either end in BACs. The bacterial transposon Tn10 carrying a loxP or a lox511 site is inserted at random into BAC DNA inside the bacterial cell. The cells are then infected with bacteriophage P1. The Cre protein expressed by phage P1 generates end-deletions by specifically recombining the inserted loxP (or lox511) with the loxP (or lox511) endogenous to and flanking insert DNA in BACs from the respective end. The Cre protein also helps phage P1 transduce the BAC DNA by packaging it in P1 heads. This packaging by P1 not only recovers the rare BAC clones containing Tn10 insertions efficiently but also selects end-truncated BACs from those containing inversions of portions of their DNA caused by transposition of Tn10 in the opposite orientation. The libraries of end-deleted BACs generated by this procedure are suitable for numerous mapping studies. Because DNA in front of the loxP (or lox511) arrowheads in the Tn10 transposon is retained at the newly created BAC end, exogenous DNA cassettes such as enhancer-traps and iTol2 ends can be efficiently introduced into BAC ends for germline expression in zebrafish or mice. The methodology should facilitate functional mapping studies of long-range cis-acting gene regulatory sequences in these organisms. PMID:25239743

Chatterjee, Pradeep K

2015-01-01

387

DNA-BASED MATCHING OF DIGITAL SIGNALS S. A. Tsaftaris, A.K. Katsaggelos, T.N. Pappas and T.E. Papoutsakis.  

E-print Network

that enables us to use a DNA based approach to digital signal matching. 1. INTRODUCTION Matching of digitalDNA-BASED MATCHING OF DIGITAL SIGNALS S. A. Tsaftaris¹, A.K. Katsaggelos¹, T.N. Pappas¹ and T to a DNA-based solution. Digital data are encoded in DNA sequences using a sophisticated codeword set

Tsaftaris, Sotirios

388

Antiproliferative effect of T/Tn specific Artocarpus lakoocha agglutinin (ALA) on human leukemic cells (Jurkat, U937, K562) and their imaging by QD-ALA nanoconjugate.  

PubMed

T/Tn specificity of Artocarpus lakoocha agglutinin (ALA), isolated from the seeds of A. lakoocha (Moraceae) fruit and a heterodimer (16 kD and 12 kD) of molecular mass 28 kD, was further confirmed by SPR analysis using T/Tn glycan containing mammalian glycoproteins. N-terminal amino acid sequence analysis of ALA showed homology at 15, 19-21, 24-27, and 29 residues with other lectin members of Moraceae family viz., Artocarpus integrifolia (jacalin) lectin, Artocarpus hirsuta lectin, and Maclura pomifera agglutinin. It is mitogenic to human PBMC and the maximum proliferation was observed at 1 ng/ml. It showed an antiproliferative effect on leukemic cells, with the highest effect toward Jurkat cells (IC(50) 13.15 ng/ml). Synthesized CdS quantum dot-ALA nanoconjugate was employed to detect the expression of T/Tn glycans on Jurkat, U937, and K562 leukemic cells surfaces as well as normal lymphocytes by fluorescence microscopy. No green fluorescence was observed with normal lymphocytes indicating that T/Tn determinants, which are recognized as human tumor associated structures were cryptic on normal lymphocyte surfaces, whereas intense green fluorescent dots appeared during imaging of leukemic cells, where such determinants were present in unmasked form. The above results indicated that QD-ALA nanoconjugate is an efficient fluorescent marker for identification of leukemic cell lines that gives rise to high quality images. PMID:18521747

Chatterjee, Urmimala; Bose, Partha Pratim; Dey, Sharmistha; Singh, Tej P; Chatterjee, Bishnu P

2008-11-01

389

ION GNSS+ 2013, Session D1, Nashville, TN, 16-20 September 2013 Page 1/15 Four-constellation Reliability in Challenging  

E-print Network

ION GNSS+ 2013, Session D1, Nashville, TN, 16-20 September 2013 Page 1/15 Four-constellation Kingdom in 2010. Her research interests include reliability of satellite navigation systems, multi-constellation of ranging signals from new GNSS constellations is expected to result in better system availability

Calgary, University of

390

Day Fire in Ventura County  

NASA Technical Reports Server (NTRS)

[figure removed for brevity, see original site] Annotated Version

The Day fire has been burning in Ventura County in Southern California since Labor Day, and has consumed more than 160,000 acres. As of September 29, it was 63 percent contained. The Advanced Spaceborne Thermal Emission and Reflection Radiometer on NASA's Terra satellite flew over the fire at 10 p.m. Pacific Time on September 28, and imaged the fire with its infrared camera. The hottest areas of active burning appear as red spots on the image. The blue-green background is a daytime image acquired in June, used as a background to allow firefighters to localize the hot spots.

With its 14 spectral bands from the visible to the thermal infrared wavelength region, and its high spatial resolution of 15 to 90 meters (about 50 to 300 feet), ASTER images Earth to map and monitor the changing surface of our planet.

ASTER is one of five Earth-observing instruments launched December 18, 1999, on NASA's Terra satellite. The instrument was built by Japan's Ministry of Economy, Trade and Industry. A joint U.S./Japan science team is responsible for validation and calibration of the instrument and the data products.

The broad spectral coverage and high spectral resolution of ASTER provides scientists in numerous disciplines with critical information for surface mapping, and monitoring of dynamic conditions and temporal change. Example applications are: monitoring glacial advances and retreats; monitoring potentially active volcanoes; identifying crop stress; determining cloud morphology and physical properties; wetlands evaluation; thermal pollution monitoring; coral reef degradation; surface temperature mapping of soils and geology; and measuring surface heat balance.

The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission directorate.

Size: 22.5 by 31.0 kilometers (12.6 by 15.2 miles) Location: 34.6 degrees North latitude, 119.1 degrees West longitude Orientation: North at top Image Data: ASTER Bands 4, 2, and 1 Original Data Resolution: ASTER 15 meters (49.2 feet) and 30 meters (98.4 feet) Dates Acquired: September 28, 2006 and June 19 2006

2006-01-01

391

Dutchess County Resource Recovery Task Force report: Dutchess County Pyrolysis Program  

SciTech Connect

Dutchess County initiated development of a long-range master plan for Solid Waste Management in 1971. The plan included development of a resource recovery facility to service the municipalities in the County population center. Based on early recommendations, a pyrolysis facility employing Purox technology was to be implemented. A feasibility study, paid for by County funds was completed in 1975. The study provided siting recommendations, estimation of available waste, and preliminary facility design. Because of various considerations, the project was not developed. Under the Department of Energy grant, the County reassessed the feasibility of a resource recovery facility, with emphasis on confirming previous conclusions supporting the Purox technology, waste availability, energy recovery and sale and siting of the plant. The conclusions reached in the new study were: a resource recovery facility is feasible for the County; sufficient waste for such a facility is available and subject to control; While Purox technology was feasible it is not the most appropriate available technoloy for the County; that mass burning with steam recovery is the most appropriate technology; and that resource recovery while presently more expensive than landfilling, represents the only cost effective, energy efficient, and environmentally sound way to handle the solid waste problem in the County.

None

1980-07-01

392

Energy Efficient Buildings, Salt Lake County, Utah  

SciTech Connect

Executive Summary Salt Lake County's Solar Photovoltaic Project - an unprecedented public/private partnership Salt Lake County is pleased to announce the completion of its unprecedented solar photovoltaic (PV) installation on the Calvin R. Rampton Salt Palace Convention Center. This 1.65 MW installation will be one the largest solar roof top installations in the country and will more than double the current installed solar capacity in the state of Utah. Construction is complete and the system will be operational in May 2012. The County has accomplished this project using a Power Purchase Agreement (PPA) financing model. In a PPA model a third-party solar developer will finance, develop, own, operate, and maintain the solar array. Salt Lake County will lease its roof, and purchase the power from this third-party under a long-term Power Purchase Agreement contract. In fact, this will be one of the first projects in the state of Utah to take advantage of the recent (March 2010) legislation which makes PPA models possible for projects of this type. In addition to utilizing a PPA, this solar project will employ public and private capital, Energy Efficiency and Conservation Block Grants (EECBG), and public/private subsidized bonds that are able to work together efficiently because of the recent stimulus bill. The project also makes use of recent changes to federal tax rules, and the recent re-awakening of private capital markets that make a significant public-private partnership possible. This is an extremely innovative project, and will mark the first time that all of these incentives (EECBG grants, Qualified Energy Conservation Bonds, New Markets tax credits, investment tax credits, public and private funds) have been packaged into one project. All of Salt Lake County's research documents and studies, agreements, and technical information is available to the public. In addition, the County has already shared a variety of information with the public through webinars, site tours, presentations, and written correspondence.

Barnett, Kimberly

2012-04-30

393

Single-molecule pair studies of the interactions of the alpha-GalNAc (Tn-antigen) form of porcine submaxillary mucin with soybean agglutinin.  

PubMed

Mucins form a group of heavily O-glycosylated biologically important glycoproteins that are involved in a variety of biological functions, including modulating immune response, inflammation, and adhesion. Mucins are also involved in cancer and metastasis and often express diagnostic cancer antigens. Recently, a modified porcine submaxillary mucin (Tn-PSM) containing GalNAcalpha1-O-Ser/Thr residues was shown to bind to soybean agglutinin (SBA) with approximately 10(6)-fold enhanced affinity relative to GalNAcalpha1-O-Ser, the pancarcinoma carbohydrate antigen. In this study, dynamic force spectroscopy is used to investigate molecular pairs of SBA and Tn-PSM. A number of force jumps that demonstrate unbinding or rebinding events were observed up to a distance equal to 2.0 microm, consistent with the length of the mucin chain. The unbinding force increased from 103 to 402 pN with increasing force loading rate. The position of the activation barrier in the energy landscape of the interaction was 0.1 nm. The lifetime of the SBA-TnPSM complex in the absence of applied force was determined to be in the range 1.3-1.9 s. Kinetic parameters describing the rate of dissociation of other sugar lectin interactions are in the range 3.3 x 10(-3)-2.5 x 10(-3) s. The long lifetime of the SBA-TnPSM complex is compatible with a binding model in which lectin molecules "bind and jump" from alpha-GalNAc residue to alpha-GalNAc residue along the polypeptide chain of Tn-PSM before dissociating. These findings have important implications for the molecular recognition properties of mucins. PMID:19384982

Sletmoen, Marit; Dam, Tarun K; Gerken, Thomas A; Stokke, Bjørn T; Brewer, Curtis Fred

2009-09-01

394

Single-Molecule Pair Studies of the Interactions of the ?-GalNAc (Tn-Antigen) Form of Porcine Submaxillary Mucin with Soybean Agglutinin  

PubMed Central

Mucins form a group of heavily O-glycosylated biologically important glycoproteins that are involved in a variety of biological functions, including modulating immune response, inflammation, and adhesion. Mucins are also involved in cancer and metastasis, and often express diagnostic cancer antigens. Recently, a modified porcine submaxillary mucin (Tn-PSM) was shown to bind to soybean agglutinin (SBA) with ~106-fold enhanced affinity relative to GalNAc?1-O-Ser, the pancarcinoma carbohydrate antigen. In the present study, dynamic force spectroscopy (DFS) is used to investigate molecular pairs of SBA and Tn-PSM. A number of force jumps that demonstrate unbinding/rebinding events were observed up to a distance equal to 2.0 micrometers, consistent with the length of the mucin chain. The unbinding force increased from 103 pN to 402 pN with increasing force loading rate. The position of the activation barrier in the energy landscape of the interaction was 0.1 nm. The lifetime of the SBA – TnPSM complex in the absence of applied force was determined to be is in the range 1.3 – 1.9 s. Kinetic parameters describing the rate of dissociation of other sugar lectin interactions are in the range 3.3 × 10?3 ? 2.5 × 10?3 s. The long lifetime of the SBA – TnPSM complex is compatible with a binding model in which lectin molecules “bind and jump” from ?-GalNAc residue to ?-GalNAc residue along the polypeptide chain of Tn-PSM before dissociating. These findings have important implications for the molecular recognition properties of mucins. PMID:19384982

Sletmoen, Marit; Dam, Tarun K.; Gerken, Thomas A.; Stokke, Bjørn T.; Brewer, C. Fred

2009-01-01

395

127. COTTONWOOD CUT AREA, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

127. COTTONWOOD CUT AREA, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, IDAHO; NORTH VIEW. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

396

128. COTTONWOOD CUT, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, IDAHO; ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

128. COTTONWOOD CUT, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, IDAHO; NORTH VIEW. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

397

125. COTTONWOOD CUT AREA, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

125. COTTONWOOD CUT AREA, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, IDAHO; SOUTH VIEW OF CANAL. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

398

68 FR 40653 - Exira Station Project in Audubon County, Iowa  

Federal Register 2010, 2011, 2012, 2013, 2014

...Notices] [Pages 40653-40654...Project in Audubon County, Iowa AGENCY: Western...fired (with fuel oil backup) electrical...Project in Audubon County, near the towns...and Brayton, Iowa. The Project...On-site [[Page 40654

2003-07-08

399

2. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

400

19. OLD HIGHWAY 101 AND ROAD VIEW. MIRANDA, HUMBOLDT COUNTY, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. OLD HIGHWAY 101 AND ROAD VIEW. MIRANDA, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

401

24. ROAD VIEW AT PRAIRE CREEK STATE PARK. HUMBOLDT COUNTY, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. ROAD VIEW AT PRAIRE CREEK STATE PARK. HUMBOLDT COUNTY, CALIFORNIA. LOOKING N. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

402

25. ROAD VIEW ON NEWTON DRURY PARKWAY. HUMBOLDT COUNTY, CALIFORNIA. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. ROAD VIEW ON NEWTON DRURY PARKWAY. HUMBOLDT COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

403

10. ROAD VIEW. SOUTH OF PIERCY, HUMBOLDT COUNTY, CALIFORNIA. OLD ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. ROAD VIEW. SOUTH OF PIERCY, HUMBOLDT COUNTY, CALIFORNIA. OLD HIGHWAY ON LEFT. NEW HIGHWAY ON RIGHT. LOOKING W. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

404

17. BENBOW INN. SOUTH OF GARBERVILLE, HUMBOLDT COUNTY, CALIFORNIA. LOOKING ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. BENBOW INN. SOUTH OF GARBERVILLE, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

405

67. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

67. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

406

15. HIGHWAY 101 BRIDGE IN RICHARDSON STATE PARK. HUMBOLDT COUNTY, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. HIGHWAY 101 BRIDGE IN RICHARDSON STATE PARK. HUMBOLDT COUNTY, CALIFORNIA. LOOKING N. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

407

11. ROAD VIEW. SOUTH OF PIERCY, HUMBOLDT COUNTY, CALIFORNIA. OLD ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. ROAD VIEW. SOUTH OF PIERCY, HUMBOLDT COUNTY, CALIFORNIA. OLD HIGHWAY ON LEFT. NEW HIGHWAY ON RIGHT. LOOKING SW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

408

Small County: An Interactive Exercise in Petroleum Exploration Geology  

NSDL National Science Digital Library

The Small County project provides students with interactive instruction in the geological aspects of petroleum reservoir characterization employing a virtual subsurface closely reflecting the geological characteristics of the US mid-continent, in the fictional setting of Small County, Kansas.

Bohling, Geoff

2010-01-01

409

THE HUNDRED CLUB OF BROWARD COUNTY ENDOWED SCHOLARSHIP  

E-print Network

THE HUNDRED CLUB OF BROWARD COUNTY ENDOWED SCHOLARSHIP 14/15 Academic Year PERSONAL INFORMATION the Hundred Club of Broward County Endowed Scholarship at UCF. Finaid.ucf.edu UCF Television Friend/Family UCF

Van Stryland, Eric

410

22. Historic American Buildings Survey Alameda County Recorder Office Map ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. Historic American Buildings Survey Alameda County Recorder Office Map Book 6, page 17 October 1960 SURVEY OF 1868 - Mission San Jose de Guadalupe, Mission & Washington Boulevards, Fremont, Alameda County, CA

411

108. MURTAUGH LAKE HEADGATES, TWIN FALLS COUNTY, SOUTH OF MURTAUGH, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

108. MURTAUGH LAKE HEADGATES, TWIN FALLS COUNTY, SOUTH OF MURTAUGH, IDAHO; OVERALL VIEW SOUTH. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

412

100. MURTAUGH LAKE HEADGATES, TWIN FALLS COUNTY, SOUTH OF MURTAUGH, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

100. MURTAUGH LAKE HEADGATES, TWIN FALLS COUNTY, SOUTH OF MURTAUGH, IDAHO; SOUTH VIEW OF HEADGATES. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

413

107. MURTAUGH LAKE, TWIN FALLS COUNTY, SOUTH OF MURTAUGH, IDAHO; ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

107. MURTAUGH LAKE, TWIN FALLS COUNTY, SOUTH OF MURTAUGH, IDAHO; WEST VIEW OF LAKE. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

414

5. TUNNEL TREE AT DRIVETHROUGHTREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. TUNNEL TREE AT DRIVE-THROUGH-TREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NE. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

415

75 FR 22892 - Environmental Impact Statement: Salt Lake County, UT  

Federal Register 2010, 2011, 2012, 2013, 2014

...Administration Environmental Impact Statement: Salt Lake County, UT AGENCY: Federal Highway...proposed transportation improvement project in Salt Lake County, Utah. FOR FURTHER INFORMATION...Administration, 2520 West 4700 South, Suite 9A, Salt Lake City, UT 84118, telephone...

2010-04-30

416

75 FR 9476 - Environmental Impact Statement: Salt Lake County, UT  

Federal Register 2010, 2011, 2012, 2013, 2014

...Administration Environmental Impact Statement: Salt Lake County, UT AGENCY: Federal Highway...proposed transportation improvement project in Salt Lake County, Utah. FOR FURTHER INFORMATION...Administration, 2520 West 4700 South, Suite 9A, Salt Lake City, UT 84118, Telephone:...

2010-03-02

417

76 FR 71936 - Upper Deckers Creek Watershed, Preston County, WV  

Federal Register 2010, 2011, 2012, 2013, 2014

...Conservation Service Upper Deckers Creek Watershed, Preston County, WV AGENCY: Natural...prepared for the Upper Deckers Creek Watershed, Preston County, West Virginia. FOR...Domestic Assistance under No. 10.904--Watershed Protection and Flood...

2011-11-21

418

Los Angeles County Poor Farm, Patient Wards 201, 202, 203, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Los Angeles County Poor Farm, Patient Wards 201, 202, 203, 204, 205, 206, 207, 208 & 209 - Type A Plan, 7601 Imperial Highway; bounded by Esperanza Street, Hawthorn Avenue, Laurel Street, and Descanso Street, Downey, Los Angeles County, CA

419

Los Angeles County Poor Farm, Patient Ward Nos. 210 & ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Los Angeles County Poor Farm, Patient Ward Nos. 210 & 211 - Type B Plan, 7601 Imperial Highway; bounded by Esperanza Street, Laurel Street, Flores Street, and Descanso Street, Downey, Los Angeles County, CA

420

Desegregation in Brazos County, Texas, 1946-1971  

E-print Network

that favored integrations their influence did not speed the process. Integration in the county was effected by the burning of several schools. Ultimately, the last district in the county fully integrated in 1971, seventeen years after the Brown decision....

Hill, Scott Ogden

2012-06-07

421

GENERAL SITE PLAN, HAMILTON AIR FORCE BASE, MARIN COUNTY, CALIFORNIA. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL SITE PLAN, HAMILTON AIR FORCE BASE, MARIN COUNTY, CALIFORNIA. Pencil on paper, dated December 4, 1952. Also marked "PWC 103474." By J.Y. Long Company, Engineers, Oakland, California - Hamilton Field, East of Nave Drive, Novato, Marin County, CA

422

Photocopy of drawing (original in possession of Allegany County, Cumberland, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original in possession of Allegany County, Cumberland, MD) CEMENT HOUSE FLOOR PLAN, 1942 - Kelly-Springfield Tire Plant, Cement House, 701 Kelly Road, Cumberland, Allegany County, MD

423

2. Photocopy of undated print (from the Chester County Historical ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Photocopy of undated print (from the Chester County Historical Society, West Chester, Pennsylvania) WEST FRONT - West Chester State Normal School, South High Street, West Chester, Chester County, PA

424

2. Photocopy of photograph (from Buffalo and Erie County Historical ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Photocopy of photograph (from Buffalo and Erie County Historical Society) Photographer unknown 1928 GENERAL VIEW FROM THE SOUTHWEST - Buffalo Lighthouse, Buffalo Harbor, Buffalo River & Lake Erie, Buffalo, Erie County, NY

425

1. Photocopy of photograph (from the Buffalo and Erie County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Photocopy of photograph (from the Buffalo and Erie County Historical Society) Photographer unknown 1859 GENERAL VIEW FROM THE SOUTHEAST - Buffalo Lighthouse, Buffalo Harbor, Buffalo River & Lake Erie, Buffalo, Erie County, NY

426

Energy & Sustainability Manager County of Sonoma, General Services, Energy & Sustainability  

E-print Network

John Haig Energy & Sustainability Manager County of Sonoma, General Services, Energy the County of Sonoma has learned, and how it can help you. #12;A Month of Energy Independence SCEIP opened of FinancingLonger Amortization of Financing #12;The Money $ Sonoma County Treasury Pool- 45 Milli $ BONDS

Kammen, Daniel M.

427

Sharpening the Focus of Yolo County Land Use Policy  

E-print Network

Sharpening the Focus of Yolo County Land Use Policy Kurt R. Richter University of California Agricultural Issues Center October 2009 #12;Sharpening the Focus of Yolo County Land Use Policy II University of California Agricultural Issues Center #12;Sharpening the Focus of Yolo County Land Use Policy III Making

Ferrara, Katherine W.

428

University of Maryland Baltimore County Office of Sponsored Programs  

E-print Network

University of Maryland Baltimore County Office of Sponsored Programs SUBRECPIENT AND VENDOR. Will the University of Maryland Baltimore County be able to complete reports about the project without that is unique to the project and minimal supervision from the PI at the University of Maryland Baltimore County

Adali, Tulay

429

Gingrich Palm Beach County supporters 'disappointed but not discouraged'  

E-print Network

Gingrich Palm Beach County supporters 'disappointed but not discouraged' 2012-02-01 17:16:03 PALM BEACH COUNTY, Fla. -- As expected Newt Gingrich lost in Florida by a landslide. The polls predicted of three created a "Palm Beach County for Newt Gingrich 2012" Facebook page in November. Shortly afterwards

Belogay, Eugene A.

430

Impacts of Health Reform in Shelby County, Tennessee  

E-print Network

after PPACA Table 10. Total Health Care Expenditures in Shelby County (Current Dollars in Millions) Table 11. Net Increases in Health Care Expenditures Under PPACA in Shelby County by Source of FundsImpacts of Health Reform in Shelby County, Tennessee An Examination of Changes in Health Insurance

Dasgupta, Dipankar

431

THE ECONOMY OF MANATEE AND SARASOTA COUNTIES Effie Philippakos  

E-print Network

i THE ECONOMY OF MANATEE AND SARASOTA COUNTIES By Effie Philippakos Alan W. Hodges David Mulkey Charles M. Adams Abstract This report is intended to characterize the economies of Manatee and Sarasota-county region. The overall size and seasonal variations in the economies of Manatee and Sarasota Counties were

Florida, University of

432

15. 'Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. 'Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919,' showing general plan, plan of top chord, elevation of main girder, transverse section, plan section at deck level. - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA

433

17. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919," showing plan of bars in top flange, elevation of girder reinforcement, plan of bars in bottom flange - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA

434

18. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

18. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919," showing elevation of center pier, elevation and plan of north and south abutments, sections of abutments, pier, and pier footings - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA

435

27 CFR 9.190 - Red Hill Douglas County, Oregon.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

2010-04-01

436

27 CFR 9.190 - Red Hill Douglas County, Oregon.  

Code of Federal Regulations, 2014 CFR

...2014-04-01 2014-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

2014-04-01

437

27 CFR 9.169 - Red Hills Lake County.  

Code of Federal Regulations, 2012 CFR

...2012-04-01 2012-04-01 false Red Hills Lake County. 9.169 Section...American Viticultural Areas § 9.169 Red Hills Lake County. (a) Name. The...viticultural area described in this section is “Red Hills Lake County”. (b) Approved...

2012-04-01

438

27 CFR 9.169 - Red Hills Lake County.  

Code of Federal Regulations, 2014 CFR

...2014-04-01 2014-04-01 false Red Hills Lake County. 9.169 Section...American Viticultural Areas § 9.169 Red Hills Lake County. (a) Name. The...viticultural area described in this section is “Red Hills Lake County”. (b) Approved...

2014-04-01

439

27 CFR 9.169 - Red Hills Lake County.  

Code of Federal Regulations, 2011 CFR

...2011-04-01 2011-04-01 false Red Hills Lake County. 9.169 Section...American Viticultural Areas § 9.169 Red Hills Lake County. (a) Name. The...viticultural area described in this section is “Red Hills Lake County”. (b) Approved...

2011-04-01

440

27 CFR 9.190 - Red Hill Douglas County, Oregon.  

Code of Federal Regulations, 2011 CFR

...2011-04-01 2011-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

2011-04-01

441

27 CFR 9.169 - Red Hills Lake County.  

Code of Federal Regulations, 2013 CFR

...2013-04-01 2013-04-01 false Red Hills Lake County. 9.169 Section...American Viticultural Areas § 9.169 Red Hills Lake County. (a) Name. The...viticultural area described in this section is “Red Hills Lake County”. (b) Approved...

2013-04-01

442

27 CFR 9.190 - Red Hill Douglas County, Oregon.  

Code of Federal Regulations, 2013 CFR

...2013-04-01 2013-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

2013-04-01

443

27 CFR 9.190 - Red Hill Douglas County, Oregon.  

Code of Federal Regulations, 2012 CFR

...2012-04-01 2012-04-01 false Red Hill Douglas County, Oregon. 9.190...American Viticultural Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name...viticultural area described in this section is “Red Hill Douglas County, Oregon”. For...

2012-04-01

444

A TURKEY NESTING STUDY IN GREGORY COUNTY, SOUTH DAKOTA  

E-print Network

A TURKEY NESTING STUDY IN GREGORY COUNTY, SOUTH DAKOTA by Tara L. Wertz A thesis submitted Sciences (Wildlife Option) South Dakota State University 1986 #12;A TURKEY NESTING STUDY IN GREGORY COUNTY. of Wildlife and Fisheries Sciences 11 Date Date #12;A TURKEY NESTING STUDY IN GREGORY COUNTY, SOUTH DAKOTA

445

REVIEW PLAN John Redmond Dam Reservoir, Coffee County, Kansas  

E-print Network

#12;#12;REVIEW PLAN John Redmond Dam Reservoir, Coffee County, Kansas Reallocation Study Tulsa PLAN John Redmond Dam Reservoir, Coffee County, Kansas Reallocation Study Tulsa District TABLE review for the Final Reallocation Report to the John Redmond Dam and Reservoir, Coffee County, Kansas

US Army Corps of Engineers

446

County-level environmental quality and associations with individual - and county-level preterm birth  

EPA Science Inventory

Human health is influenced by simultaneous exposure to stressors and amenities, but research usually considers single exposures. We constructed a county-level Environmental Quality Index (EQI) using principal components analysis with data from five domains (air, water, land, buil...

447

59 FR- East Shore Project, Lake Tahoe Basin Management Unit (LTBMU), Washoe County, Douglas County, and...  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF AGRICULTURE Forest Service East Shore Project, Lake Tahoe Basin Management Unit...County, and Carson City Rural Area, NV AGENCY: Forest Service, USDA...made by Lake Tahoe Basin Management Unit Forest Supervisor,...

1994-01-14

448

About Mobile County Public School System Mobile County Public School System (MCPSS) has an enrollment of  

E-print Network

hospitality of many of its better-known Gulf Coast neighbors. The county, which hosts America's oldest Mardi Gras celebration, celebrates its bicentennial this year. It was originally home to the Choctaw

Fisher, Kathleen

449

A new ?-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for ?-galactosidase activity of gastrointestinal bacteria.  

PubMed

The ?-galactosidase gene, galA17, was cloned from Flavobacterium sp. TN17, a symbiotic bacterium isolated from the gut of Batocera horsfieldi larvae. The 2,205-bp full-length gene encodes a 734-residue polypeptide (GalA17) containing a putative 28-residue signal peptide and a catalytic domain belonging to glycosyl hydrolase family 36 (GH 36). The deduced amino acid sequence of galA17 was most similar to a putative ?-galactosidase from Pedobacter sp. BAL39 (EDM38577; 66.6% identity) and a characterized ?-galactosidase from Carnobacterium piscicola BA (AAL27305; 30.1%). Phylogenetic analysis revealed that GalA17 was similar to GH 36 ?-galactosidases from symbiotic bacteria sharing two putative catalytic motifs, KWD and SDXXDXXXR, in which D480, S548, D549, and R556 were essential for ?-galactosidase activity based on site-directed mutagenesis. Purified recombinant GalA17 showed apparent optimal activity at pH 5.5 and 45°C; exhibited strong resistance to digestion by trypsin, ?-chymotrypsin, collagenase, and proteinase K; and efficiently hydrolyzed several synthetic and natural substrates (p-nitrophenyl-?-D-galactopyranoside, stachyose, melibiose, raffinose, soybean meal, locust bean gum, and guar gum). PMID:20714719

Zhou, Junpei; Shi, Pengjun; Huang, Huoqing; Cao, Yanan; Meng, Kun; Yang, Peilong; Zhang, Rui; Chen, Xiaoyan; Yao, Bin

2010-12-01

450

Preclinical evaluation of a CXCR4-specific (68)Ga-labelled TN14003 derivative for cancer PET imaging.  

PubMed

Molecular imaging is an ideal platform for non-invasive detection and assessment of cancer. In recent years, the targeted imaging of CXCR4, a chemokine receptor that has been associated with tumour metastasis, has become an area of intensive research. In our pursuit of a CXCR4-specific radiotracer, we designed and synthesised a novel derivative of the CXCR4 peptidic antagonist TN14003, CCIC16, which is amenable to radiolabelling by chelation with a range of PET and SPECT radiometals, such as (68)Ga, (64)Cu and (111)In as well as (18)F (Al(18)F). Potent in vitro binding affinity and inhibition of signalling-dependent cell migration by unlabelled CCIC16 were confirmed by a threefold uptake in CXCR4-over-expressing cells compared to their isogenic counterparts. Furthermore, in vivo experiments demonstrated the favourable pharmacokinetic properties of the (68)Ga-labelled tracer (68)Ga-CCIC16, along with its CXCR4-specific accumulation in tissues with desirable contrast (tumour-to-muscle ratio: 9.5). The specificity of our tracer was confirmed by blocking experiments. Taking into account the attractive intrinsic PET imaging properties of (68)Ga, the comprehensive preclinical evaluation presented here suggests that (68)Ga-CCIC16 is a promising PET tracer for the specific imaging of CXCR4-expressing tumours. PMID:24365390

George, Guillaume P C; Stevens, Elizabeth; Åberg, Ola; Nguyen, Quang-Dé; Pisaneschi, Federica; Spivey, Alan C; Aboagye, Eric O

2014-01-15

451

TV and Extension in Carbon County, Pa.  

ERIC Educational Resources Information Center

To help improve televised extension education, a study was made of the interests and characteristics of the potential audience (9,300) of PTVC, a community antenna television system in Carbon County, Pennsylvania. A checklist questionnaire survey drew 160 usable responses from the communities of Jim Thorpe, Lehighton, and Palmerton. Data on…

Reitz, Ray W.

452

Hazlit Creek field, Wilkinson County, Mississippi  

Microsoft Academic Search

The Hazlit Creek field in NW. Wilkinson County, Mississippi, about 30 miles (48 km) south of Natchez, is an excellent example of an Eocene Wilcox oil field discovered by subsurface mapping and stratigraphic studies. The field was discovered in March 1966 and now contains 22 producing oil wells; only 5 dry holes have been drilled north and east of the

G. W. Gulmon; A. T. Jr. Ricci; H. E. Hansen

1972-01-01

453

The black death in county Durham  

Microsoft Academic Search

The purpose of this article is twofold. First, it attempts to estimate the number of deaths in percentage terms which County Durham may have experienced in 1349 during the first outbreak of the plague and to compare this with what happened in other parts of England. Following this is a consideration of the length of time over which the plague

Richard Lomas

1989-01-01

454

Monroe County Extension Services Key West Office  

E-print Network

and the "Monroe County Extension IFAS Blog" http://blogs.ifas.ufl.edu/monroeco Stay up-to-date with the latest to: http://trec.ifas.ufl.edu/fruitscapes For palm care and pruning, visit: http://flrec.ifas.ufl.edu; look in left column for Palm Production and Maintenance. The Institute of Food and Agricultural

Florida, University of

455

MORES CREEK STUDY, BOISE COUNTY, IDAHO, 1979  

EPA Science Inventory

In Water Year 1979, a water quality study was conducted on Mores Creek in Boise County, Idaho (17050112) to determine the present water quality of the stream and obtain background information on effluent limitations development for Idaho City. The study was designed for approxim...

456

ELK CREEK STUDY, IDAHO COUNTY IDAHO, 1979  

EPA Science Inventory

In Water Year 1979, the American River, the Red River, and Elk Creek in Idaho County (17060305) were studied to determine their present water quality and to obtain background information on effluent limitations development for the Elk City sewage treatment plant. Quarterly monit...

457

CAMAS CREEK STUDY, CAMAS COUNTY, IDAHO. 1979  

EPA Science Inventory

The National Eutrophication Survey on Magic Reservoir determined that Camas Creek in Camas County, Idaho (17040220) contributed roughly 45% of the total phosphorus load and 34% of the total nitrogen load into Magic Reservoir. From this finding, a water quality study was conducte...

458

DEARY CREEK STUDY, LATAH COUNTY, IDAHO. 1979  

EPA Science Inventory

In Water Year 1979, a water quality study was conducted on Mount Deary Creek in Latah County, Idaho (17060306) to determine the present water quality of the stream and to obtain background information to determine effluent limitations for the City of Deary. The survey involved t...

459

Retrofit LA (County of Los Angeles)  

E-print Network

centers, community college districts, local economic development programs, Build It Green, and others: refine marketing and outreach strategies; develop workshops for directing stakeholders and contractors an innovative marketing and outreach strategy to motivate property owners to participate. The County of Los

460

Deer Lodge County Secondary Data Analysis  

E-print Network

Deer Lodge County Secondary Data Analysis July 23, 2012 1 1 Community Health Data, MT Myocardial Infarction prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1, Granite, Powell, Deer Lodge, Jefferson, Broadwater, Meagher, Silver Bow, Gallatin, Park, Madison

Maxwell, Bruce D.

461

Lewis & Clark County Secondary Data Analysis  

E-print Network

Myocardial Infarction prevalence (Heart Attack) 3.3% 4.1% 6.0% All Sites Cancer 416.6 (Region 4) 455) ­ Lewis and Clark, Granite, Powell, Deer Lodge, Jefferson, Broadwater, Meagher, Silver Bow, Gallatin of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease 3

Maxwell, Bruce D.

462

Volusia County, Florida. PLATO Evaluation Series.  

ERIC Educational Resources Information Center

An evaluation was conducted to determine how the PLATO Pathways program was used in three high schools in Volusia County, Florida: Deland, New Smyrna Beach, and Atlantic. Of the many PLATO sites in Volusia, these three were selected for study because of the representative patterns of low, medium, and high PLATO use. PLATO is a computer learning…

Hannafin, Bob

463

Lycoming County Women's History Curriculum Guide  

ERIC Educational Resources Information Center

This project plays upon technology and an innovative way to research history -- the use of online primary source data that highlight the history of women in volunteer and reform organizations, education, the arts, the workplace, and private lives. Using an archival database from the Lycoming County Women's History collection, this curriculum…

Rogers, Amy

2011-01-01

464

Grand County Council June 6, 2006  

E-print Network

Nate Knight, Jim Lewis, Rex Tanner, Judy Carmichael, Jerry McNeely, Audrey Graham with Grand County Clerk/Auditor Fran Townsend and Diana Carroll. Elected Official Reports A. Discussion of Need and they should be filed in that office. Happy requested that Diana Carroll and Pat Byrd attend the training

Tipple, Brett

465

Food control concept: A county perspective  

SciTech Connect

This paper explains the Benton County, Washington responsibilities for implementing the ingestion exposure pathway protective actions. These responsibilities were successfully demonstrated in the September 13-14, 1994 FEMA evaluated exercise involving the Washington Public Power Supply System`s WNP-2 commercial nuclear power reactor.

Calvert, B.E. [Benton County Emergency Management, WA (United States)

1995-12-31

466

Geothermal development plan: northern Arizona counties  

SciTech Connect

The Northern Counties Area Development Plan evaluated the regional market potential for utilizing geothermal energy. This study identified five potential geothermal resource areas, four of which have low temperature (<90{sup 0}C, 194{sup 0}F) potential and one possible igneous system. The average population growth rate in the Northern Counties is expected to be five percent per year over the next 40 years, with Mohave and Yavapai Counties growing the fastest. Rapid growth is anticipated in all major employment sectors, including trade, service, manufacturing, mining and utilities. A regional energy use analysis is included, containing information on current energy use patterns for all user classes. Water supplies are expected to be adequate for expected growth generally, though Yavapai and Gila Counties will experience water deficiencies. A preliminary district heating analysis is included for the towns of Alpine and Springerville. Both communities are believed located on geothermal resource sites. The study also contains a section identifying potential geothermal resource users in northern Arizona.

White, D.H.; Goldstone, L.A.

1982-08-01

467

OROFINO CREEK STUDY, CLEARWATER COUNTY IDAHO, 1979  

EPA Science Inventory

In Water Year 1979, a water quality study was conducted on Orofino Creek in Clearwater County, Idaho (17060306) to determine the present condition of the stream and to assess the impact of point and nonpoint sources. The study involved approximately bi-monthly monitoring for the...

468

Engineering materials of northern New Castle County  

USGS Publications Warehouse

This investigation was undertaken to locate deposits of rock, sand, gravel, fill and borrow in northern New Castle County which may be potential sources of material for highway construction, and to prepare maps and descriptions of the surficial earth materials relative to their geologic and engineering properties.

Ward, R.F.; Groot, J.J.

1957-01-01

469

Windham County Natural Resources Conservation District  

Microsoft Academic Search

The Windham County Natural Resources Conservation District was authorized and formed by the State of Vermont on January 8, 1946, under the provisions of the Soil Conservation Act No. 246 of the Acts of the 1939 Vermont General Assembly. It is one of 14 Conservation Districts in the state of Vermont. Windham District is a political subdivision of the State

Vice Chairperson; Gail MacArthur; Andrea Darrow

470

Wind Energy Guide for County Commissioners  

Microsoft Academic Search

One of the key stakeholders associated with economic development are local government officials, who are often required to evaluate and vote on commercial wind energy project permits, as well as to determine and articulate what wind energy benefits accrue to their counties. Often these local officials lack experience with large-scale wind energy and need to make important decisions concerning what

Costanti

2006-01-01

471

Yellowstone County Health Manpower and Education Profile.  

ERIC Educational Resources Information Center

The profile is a concise description of the demographic and economic characteristics, existing health manpower employed, and health education programs for the Yellowstone County area of Montana, one of seven surveyed in the Mountain States region (Idaho, Montana, Wyoming, and Nevada). The first section of the profile provides general population…

Callen, John; And Others

472

Missoula County Health Manpower and Education Profile.  

ERIC Educational Resources Information Center

The profile is a concise description of the demographic and economic characteristics, existing health manpower employed, and health education programs for the Missoula County area of Montana, one of seven surveyed in the Mountain States region (Idaho, Montana, Wyoming, and Nevada). The first section of the profile provides general population…

Callen, John; And Others

473

Sheridan County Health Manpower and Education Profile.  

ERIC Educational Resources Information Center

The profile is a concise description of the demographic and economic characteristics, existing health manpower employed, and health education programs for the Sheridan County area of Wyoming, one of seven surveyed in the Mountain States region (Idaho, Montana, Wyoming, and Nevada). The first section of the profile provides general population…

Callen, John; And Others

474

Clark County Health Manpower and Education Profile.  

ERIC Educational Resources Information Center

The profile is a concise description of the demographic and economic characteristics, existing health manpower employed, and health education programs for the Clark County area of Nevada, one of seven surveyed in the Mountain States region (Idaho, Montana, Wyoming, and Nevada). The first section of the profile provides general population…

Callen, John; And Others

475

The Topic Is the County Parks.  

ERIC Educational Resources Information Center

Designed for use by teachers, students, and bus drivers, this guidebook provides suggestions, background information, and exercises to prepare participants to take part in New Jersey's County Park Program. First, scheduling information, recommendations concerning clothing, checklists of things to bring, safety tips, and a map are presented. Next,…

Grant, David; And Others

476

Lee County Government Division of Natural Resources  

E-print Network

County that also rely directly good water quality... Fisheries, Real Estate, etc... #12;Caloosahatchee ·Our Water Quality Restoration Mandate ·What Are TMDLs & BMAPs? ·Cost of Water Quality Restoration ·Expenditures for Water Quality Ecosystem Services ·How Do We Meet These Obligations? ·Source Control

Demers, Nora Egan

477

DesotoCounty Number of volunteers 132  

E-print Network

production; Florida Cattleman's Institute and Allied Trade Show; Florida deer and turkey short course agricultural and related industries generate 7,354 jobs (55.9% of total) in DeSoto County. $0.40 billion in revenues. 57.3% contribution to gross regional product. Based on a 2010 UF study to the community. $1

Ma, Lena

478

Orange County Outdoor School: Cabin Leader's Manual.  

ERIC Educational Resources Information Center

Presented in five sections, the manual furnishes cabin leaders (high school students) with background information concerning philosophy, teaching, objectives, daily schedule, and cabin leader responsibilities in the Orange County Outdoor School program. The welcome section contains the history of the Outdoor School, staff responsibilities,…

Orange County Dept. of Education, Santa Ana, CA.

479

Orange County Outdoor School: Teacher's Guide.  

ERIC Educational Resources Information Center

Divided into six sections, the guide provides helpful information for the teacher to prepare students to attend the Orange County Outdoor School. Pre-camp responsibilities section provides pre-camp preparation checklists for the principal, teacher, parents, school nurse, and outdoor specialist; a checklist for morning departure; discipline policy…

Orange County Dept. of Education, Santa Ana, CA.

480

2013 4-H County Events Photography Contest  

E-print Network

2013 4-H County Events Photography Contest #12;Senior Photos #12;Preston Strickland-Animal #12;Hannah Nistler-Architectural #12;Tara Holtzendorf-Architectural #12;Tara Holtzendorf-Animal #12;Tara Merritt-Scenic #12;Justin Parrish-Still Life #12;Kayla Moss-Plant #12;Kaylee Leshuk-Architectural #12

Hill, Jeffrey E.

481

Marcellus Shale Outcrop in Highland County, Virginia  

USGS Multimedia Gallery

A Marcellus Shale outcrop in Highland County, Virginia, shows at the surface the object of shale gas development drilling in nearby states. Assessing undiscovered gas resources, like the USGS 2011 assessment of the Marcellus Shale, uses geologic mapping of outcrops like this in addition to extensive...

482

County Business Patterns: United States, 2002.  

ERIC Educational Resources Information Center

In this report, subnational economic data by industry, including Educational Services, is provided. County Business Patterns is useful for studying the economic activity of small areas; analyzing economic changes over time; and as a benchmark for statistical series, surveys, and databases between economic censuses. The number of establishments,…

US Department of Commerce, 2004

2004-01-01

483

Remembering Jack: A Hampshire County Summer.  

ERIC Educational Resources Information Center

A former counselor at a summer camp in Hampshire County (West Virginia) recalls his experiences with his campers and Jack Schaffenaker, an Appalachian "mountain man" and guitar picker. Through Jack, the campers engaged in fishing, storytelling, hiking, and folk singing, and shed some negative stereotypes about Appalachian people. (KS)

Olson, Ted

1993-01-01

484

A Profile of Ashe County, North Carolina.  

ERIC Educational Resources Information Center

From 1950 to 1970, the shift from agriculture to industry dominated Ashe County, North Carolina, isolated on the Blue Ridge by rugged terrain and severe weather. Rural farm population declined by 2/3 but rural non-farm population tripled. Many new industries helped shift the bulk of the work force to industry. In 1950, 45% of the work force farmed…

Rash, James O., Jr.; And Others

485

Cumberland County College, AACC Progress Report.  

ERIC Educational Resources Information Center

In 1996, Cumberland County College (New Jersey) participated in the American Association of Community Colleges' Exploring America's Communities project, which works to strengthen the teaching and learning of American history, literature, and culture at U.S. community colleges. The primary goals of the college's action plan were: emphasizing…

Cumberland County Coll., Vineland, NJ.

486

[A Profile of Union County, South Carolina].  

ERIC Educational Resources Information Center

Now almost totally dependent on textile production, heavily forested Union County, South Carolina, was primarily agricultural until the 20th century. By 1970, 65% of the population depended on manufacturing and only 4% of the workers on farming. From 1920 to 1970 the population was characterized by a rural-to-urban shift and by outmigration,…

Lilley, Stephen C.; McLean, Edward L.

487

Eight Americas: Investigating Mortality Disparities across Races, Counties, and Race-Counties in the United States  

Microsoft Academic Search

BackgroundThe gap between the highest and lowest life expectancies for race-county combinations in the United States is over 35 y. We divided the race-county combinations of the US population into eight distinct groups, referred to as the “eight Americas,” to explore the causes of the disparities that can inform specific public health intervention policies and programs.Methods and FindingsThe eight Americas

Christopher J. L. Murray; Sandeep C. Kulkarni; Catherine Michaud; Niels Tomijima; Maria T. Bulzacchelli; Terrell J. Iandiorio; Majid Ezzati

2006-01-01

488

Illinois Kids Count: A Snap Shot of Our Future. County by County Profiles of Child Well-Being '92.  

ERIC Educational Resources Information Center

This booklet presents statistics concerning the well-being of Illinois' 3.3 million children between 1980 and 1990. Statistics are compared county by county for each of the state's 102 counties, and statewide statistics are compared with those of the entire nation. A statewide analysis focuses on spending per pupil on education, the percentage of…

Voices for Illinois Children, Chicago.

489

Polyvalency of Tn (GalNAc?1?Ser\\/Thr) glycotope as a critical factor for Vicia villosa B 4 and glycoprotein interactions  

Microsoft Academic Search

Vicia villosa B4 (VVL-B4) is an important lectin for detecting exposed Tn (GalNAc?1-Ser\\/Thr) determinants on cancer cells. In order to elucidate the binding factors involved in VVL-B4 and glycotope interaction, the binding properties of this lectin were analyzed by enzyme-linked lectinosorbent and inhibition assays. From the results, it is concluded that the most critical factor affecting VVL-B4 binding is polyvalency

Albert M. Wu

2004-01-01

490

Monoclonal antibodies against the Tn-specific isolectin B4 from Vicia villosa seeds: characterization of the epitope of the blocking antibody VV34.  

PubMed

Vicia villosa isolectin B4 (VVLB4) recognizes the Tn antigen (GalNAc-O-Ser/Thr) exposed in certain human carcinomas. We have produced anti-VVLB4 monoclonal antibodies (MAbs), and their lectin recognition selectivity was assessed by ELISA and Western blot against the purified Gal/GalNAc-specific lectins from Vicia villosa, Salvia sclarea, Helix pomatia, Arachis hypogaea, Glycine max, and Dolichos biflorus. The antibodies were also tested for their ability to block the binding of VVLB4 to the Tn antigen expressed on immobilized asialo ovine submaxillary mucin. Two MAbs, VV34 and VV2, specifically recognized VVLB4 and impaired the binding of the lectin to the Tn antigen by 98% and 21%, respectively. On the other hand, MAbs VV1 and VV22 cross-reacted with other purified lectins. The four antibodies recognized native and periodate-oxidized nonreduced as well as reduced VVLB4 after SDS-PAGE and Western blot, suggesting that they were recognizing continuous polypeptide epitopes. The VV34 antibody recognized two tryptic peptides (7-29 and 96-106) from VVLB4, which are contiguous in the three-dimensional structure of the lectin. The minimum structural determinant of the epitope was mapped to the polypeptide stretch (18)LILQED(23) using a set of overlapping synthetic peptides. This region of the molecule encompasses the C-terminal part of the loop joining strands beta1 and beta2 and the N-terminal part of beta2, and is located about 20-25 A away from the center of the Tn-combining site. PMID:15000847

Medeiros, Andrea; Berois, Nora; Balter, Henia; Robles, Ana; Perez-Payá, Enrique; Gimenez, Ana; Calvete, Juan J; Osinaga, Eduardo

2004-02-01

491

Simultaneous detection and removal of organomercurial compounds by using the genetic expression system of an organomercury lyase from the transposon Tn MERI1  

Microsoft Academic Search

Using a newly identified organomercury lyase gene (merB3) expression system from TnMERI1, the mercury resistance transposon first found in Gram-positive bacteria, a dual-purpose system to detect and remove organomercurial contamination was developed. A plasmid was constructed by fusing the promoterless luxAB genes as bioluminescence reporter genes downstream of the merB3 gene and its operator\\/promoter region. Another plasmid, encoding mer operon

M. Narita; T. Yamagata; H. Ishii; C.-C. Huang; G. Endo

2002-01-01

492

Effect of the polymer concentration on the ON/OFF states of a TN-LCD: polyvinyl alcohol vs. soy lecithin  

NASA Astrophysics Data System (ADS)

In this work we study the response of a Twisted Nematic Liquid Crystal Display (TN-LCD) by varying both the concentration and the polymer used for the microgroove. We compare the performance of two polymers: polyvinyl alcohol and soy lecithin. In particular, the light transmission for the ON/OFF states is evaluated. The polyvinyl alcohol is a polymer widely used in LCDs while lecithin soy is a natural polymer.

de Coss Martinez, Romeo; Gonzalez Murguia, Jose Luis

2011-03-01

493

Morris County Improvement Authority, Morris County, New Jersey Renewable Energy Initiative  

SciTech Connect

The Morris County Improvement Authority (?Authority?), a public body corporate and politic of the State of New Jersey and created and controlled by the County, at the direction of the County and through the Program guaranteed by the County, financed 3.2 MW of solar projects (?Solar Projects?) at fifteen (15) sites for seven (7) local government units (?Local Units?) in and including the County. The Program uses a Power Purchase Agreement (?PPA?) structure, where the Solar Developer constructs, operates and maintains all of the Solar Projects, for the benefit of the Local Units and the Authority, for the maximum State law allowable PPA period of fifteen (15) years. Although all fifteen (15) sites were funded by the Authority, only the Mennen Arena site was considered for the purposes of the required local match funding for this grant. Specifically at the Mennen Arena site, the Authority financed 1.6 MW of solar panels. On October 18, 2013, the DOE Grant was drawn down following completion of the necessary application documents and final execution of an agreement memorializing the contemplated transaction by the Local Units, the County, The Authority and the solar developer. The proceeds of the DOE Grant were then applied to reduce the PPA price to all Local Units across the program and increase the savings from approximately 1/3 to almost half off the existing and forecasted utility pricing over the fifteen (15) year term, without adversely affecting all of the other benefits. With the application of the rate buy down, the price of electricity purchased under the PPA dropped from 10.9 to 7.7 cents/kWh. This made acquisition of renewable energy much more affordable for the Local Units, and it enhanced the success of the program, which will encourage other counties and local units to develop similar programs.

Bonanni, John [Chair, Morris County Improvement Authority] Chair, Morris County Improvement Authority

2013-05-01

494

Comparisons of cat and dog rabies vaccination rates between epizootic to non-epizootic counties and urban to rural counties in the state of Texas  

E-print Network

come into contact with wildlife. This study was undertaken compare the vaccination rates of cats and dogs the epizootic counties the non-epizootic counties to see if the rabies increase vaccination of cats and dogs the epizootic counties. Comparisons...

Martin-Harborth, Michelle Lynn

2012-06-07

495

Identification and characterization of non-cellulose-producing mutants of Gluconacetobacter hansenii generated by Tn5 transposon mutagenesis.  

PubMed

The acs operon of Gluconacetobacter is thought to encode AcsA, AcsB, AcsC, and AcsD proteins that constitute the cellulose synthase complex, required for the synthesis and secretion of crystalline cellulose microfibrils. A few other genes have been shown to be involved in this process, but their precise role is unclear. We report here the use of Tn5 transposon insertion mutagenesis to identify and characterize six non-cellulose-producing (Cel(-)) mutants of Gluconacetobacter hansenii ATCC 23769. The genes disrupted were acsA, acsC, ccpAx (encoding cellulose-complementing protein [the subscript "Ax" indicates genes from organisms formerly classified as Acetobacter xylinum]), dgc1 (encoding guanylate dicyclase), and crp-fnr (encoding a cyclic AMP receptor protein/fumarate nitrate reductase transcriptional regulator). Protein blot analysis revealed that (i) AcsB and AcsC were absent in the acsA mutant, (ii) the levels of AcsB and AcsC were significantly reduced in the ccpAx mutant, and (iii) the level of AcsD was not affected in any of the Cel(-) mutants. Promoter analysis showed that the acs operon does not include acsD, unlike the organization of the acs operon of several strains of closely related Gluconacetobacter xylinus. Complementation experiments confirmed that the gene disrupted in each Cel(-) mutant was responsible for the phenotype. Quantitative real-time PCR and protein blotting results suggest that the transcription of bglAx (encoding ?-glucosidase and located immediately downstream from acsD) was strongly dependent on Crp/Fnr. A bglAx knockout mutant, generated via homologous recombination, produced only ?16% of the wild-type cellulose level. Since the crp-fnr mutant did not produce any cellulose, Crp/Fnr may regulate the expression of other gene(s) involved in cellulose biosynthesis. PMID:24013627

Deng, Ying; Nagachar, Nivedita; Xiao, Chaowen; Tien, Ming; Kao, Teh-hui

2013-11-01

496

Hfq restructures RNA-IN and RNA-OUT and facilitates antisense pairing in the Tn10/IS10 system  

PubMed Central

Hfq functions in post-transcriptional gene regulation in a wide range of bacteria, usually by promoting base-pairing of mRNAs and trans-encoded sRNAs that share partial sequence complementarity. It is less clear if Hfq is required for pairing of cis-encoded RNAs (i.e., antisense RNAs) with their target mRNAs. In the current work, we have characterized the interactions between Escherichia coli Hfq and the components of the Tn10/IS10 antisense system, RNA-IN and RNA-OUT. We show that Hfq interacts with RNA-OUT through its proximal RNA-binding surface, as is typical for Hfq and trans-encoded sRNAs. In contrast, RNA-IN binds both proximal and distal RNA-binding surfaces in Hfq with a higher affinity for the latter, as is typical for mRNA interactions in canonical sRNA-mRNA pairs. Importantly, an amino acid substitution in Hfq that interferes with RNA binding to the proximal site negatively impacts RNA-IN:OUT pairing in vitro and suppresses the ability of Hfq to negatively regulate IS10 transposition in vivo. We also show that Hfq binding to RNA-IN and RNA-OUT alters secondary structure elements in both of these RNAs and speculate that this could be important in how Hfq facilitates RNA-IN:OUT pairing. Based on the results presented here, we suggest that Hfq could be involved in regulating RNA pairing in other antisense systems, including systems encoded by other transposable elements. PMID:23510801

Ross, Joseph A.; Ellis, Michael J.; Hossain, Shahan; Haniford, David B.

2013-01-01

497

A novel low-temperature-active ?-glucosidase from symbiotic Serratia sp. TN49 reveals four essential positions for substrate accommodation.  

PubMed

A 2,373-bp full-length gene (bglA49) encoding a 790-residue polypeptide (BglA49) with a calculated mass of 87.8 kDa was cloned from Serratia sp. TN49, a symbiotic bacterium isolated from the gut of longhorned beetle (Batocera horsfieldi) larvae. The deduced amino acid sequence of BglA49 showed the highest identities of 80.1% with a conceptually translated protein from Pantoea sp. At-9b (EEW02556), 38.3% with the identified glycoside hydrolase (GH) family 3 ?-glucosidase from Clostridium stercorarium NCBI 11754 (CAB08072), and <15.0% with the low-temperature-active GH 3 ?-glucosidases from Shewanella sp. G5 (ABL09836) and Paenibacillus sp. C7 (AAX35883). The recombinant enzyme (r-BglA49) was expressed in Escherichia coli and displayed the typical characteristics of low-temperature-active enzymes, such as low temperature optimum (showing apparent optimal activity at 35°C), activity at low temperatures (retaining approximately 60% of its maximum activity at 20°C and approximately 25% at 10°C). Compared with the thermophilic GH 3 ?-glucosidase, r-BglA49 had fewer hydrogen bonds and salt bridges and less proline residues. These features might relate to the increased structure flexibility and higher catalytic activity at low temperatures of r-BglA49. The molecular docking study of four GH 3 ?-glucosidases revealed five conserved positions contributing to substrate accommodation, among which four positions of r-BglA49 (R192, Y228, D260, and E449) were identified to be essential based on site-directed mutagenesis analysis. PMID:21559826

Zhou, Junpei; Zhang, Rui; Shi, Pengjun; Huang, Huoqing; Meng, Kun; Yuan, Tiezheng; Yang, Peilong; Yao, Bin

2011-10-01

498

Impacts of cool cities on air quality: A preliminary modeling assessment for Nashville TN, Dallas TX and Atlanta GA  

SciTech Connect

Previous atmospheric modeling efforts that concentrated on the Los Angeles Basin suggested beneficial and significant air quality impacts from cool cities strategies. This paper discusses an extension of similar modeling efforts to three regions, Atlanta GA, Dallas - Ft. Worth TX, and Nashville TN, that experience smog and air quality problems. According to the older ozone air quality standard (120 ppb), these regions were classified as serious, moderate, and marginal, respectively, but may be out of compliance with respect to the newer, 80-ppb/8-hours standard. Results from this exploratory modeling work suggest a range of possible impacts on meteorological and air quality conditions. For example, peak ozone concentrations during each region's respective episode could be decreased by 1-6 ppb (conservative and optimistic scenarios, respectively) in Nashville, 5-15 ppb in Dallas - Fort Worth, and 5-12 ppb in Atlanta following implementation of cool cities. The reductions are generally smaller than those obtained from simulating the Los Angeles Basin but are still significant. In all regions, the simulations suggest, the net, domain-wide effects of cool cities are reductions in ozone mass and improvements in air quality. In Atlanta, Nashville, and Dallas, urban areas benefiting from reduced smog reach up to 8460, 7350, and 12870 km{sup 2} in area, respectively. Results presented in this paper should be taken as exploratory and preliminary. These will most likely change during a more comprehensive modeling study to be started soon with the support of the US Environmental Protection Agency. The main purpose of the present project was to obtain the initial data (emission inventories) for these regions, simulate meteorological conditions, and perform preliminary sensitivity analysis. In the future, additional regions will be simulated to assess the potential of cool cities in improving urban air quality.

Taha, Haider

1998-06-15

499

The Research on the Status of Small-and-Medium-Sized Enterprises of County in the Dominant Industry of Shaanxi County Economy  

Microsoft Academic Search

The thesis analyzes the role of small-and-medium-sized enterprises of county in promoting county economy: the small-and-medium-sized enterprises of county become the material basis of developing production and boosting county economy; the small-and-medium-sized enterprises of county become the main channel of increasing farmers’ job opportunities and realizing affluent life; the development of the small-and-medium-sized enterprises of county become the propeller of

Tao Jiang

2010-01-01

500

King County Earthworks: Land Reclamation as Sculpture  

NSDL National Science Digital Library

Can land reclamation be public art? It most certainly can and large scale installations can be found in abundance from California to Maine. This rather wonderful project appeared in Washington's King County in the summer of 1979. The King County Archives brought together original interviews, photos, and other documents for this marvelous exploration of a unique and trend-setting endeavor. The intent of the project was to have artists create various earthworks on land that had been environmentally damaged. The first phase was a demonstration project, then other artists moved on to create six different sites. The first demonstration project was crafted by well-known artist Robert Morris and there are a number of videos, explanatory essays, and diagrams documenting this crafty and fascinating experiment in public art.

2013-11-04