Sample records for sevier county tn

  1. 77 FR 67057 - CSX Transportation, Inc.-Discontinuance of Service Exemption-in Anderson County, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-08

    ...Transportation, Inc.--Discontinuance of Service Exemption--in Anderson County, TN CSX Transportation, Inc. (CSXT) filed a verified...milepost 0AE 256.0 at the end of the track in Oak Ridge, Anderson County, Tenn. The line traverses United States Postal...

  2. Geological and petrophysical characterization of the Ferron Sandstone for 3-D simulation of a fluvial-deltaic reservoir. Deliverable 2.5.4, Ferron Sandstone lithologic strip logs, Emergy & Sevier Counties, Utah: Volume I

    SciTech Connect

    Allison, M.L.

    1995-12-08

    Strip logs for 491 wells were produced from a digital subsurface database of lithologic descriptions of the Ferron Sandstone Member of the Mancos Shale. This subsurface database covers wells from the parts of Emery and Sevier Counties in central Utah that occur between Ferron Creek on the north and Last Chance Creek on the south. The lithologic descriptions were imported into a logging software application designed for the display of stratigraphic data. Strip logs were produced at a scale of one inch equals 20 feet. The strip logs were created as part of a study by the Utah Geological Survey to develop a comprehensive, interdisciplinary, and qualitative characterization of a fluvial-deltaic reservoir using the Ferron Sandstone as a surface analogue. The study was funded by the U.S. Department of Energy (DOE) under the Geoscience/Engineering Reservoir Characterization Program.

  3. Extensional collapse along the Sevier Desert reflection, northern Sevier Desert basin, western United States

    NASA Astrophysics Data System (ADS)

    Coogan, James C.; Decelles, Peter G.

    1996-10-01

    Newly released and previously published seismic reflection data from the northern Sevier Desert basin provide a complete seismic transect between the tilted western margin of the basin and the eastern breakaway zone. When tied to well and surface age data, the transect delineates a continuum of extensional fault and basin fill geometries that developed between late Oligocene and Pleistocene time across the basin. A minimum of 18 km of top-to-the-west normal displacement is estimated across the Sevier Desert from only the most conspicuous growth geometries and offsets across listric normal faults that sole downward into the Sevier Desert reflection (SDR). The SDR clearly marks a normal fault zone beneath the entire basin, where stratal truncations are imaged for 50% of the 39 km length of the reflection east of the Cricket Mountains block. Restoration of extensional displacement along this entire 39 km fault length is necessary to reconstruct the pre-Oligocene configuration and erosion level of Sevier thrust sheets across the Sevier Desert area. The SDR normal fault zone underlies the former topographic crest of the Sevier orogenic belt, where it accommodated extensional collapse after cessation of regional contractile tectonism.

  4. Superfund record of decision amendment (EPA Region 4): Wrigley Charcoal Superfund Site, Hickman County, Wrigley, TN, February 2, 1995

    SciTech Connect

    NONE

    1995-03-01

    This decision document presents the selected Interim Remedial Action (IRA) for the Wrigley Charcoal Site, in Wrigley, Hickman County, Tennessee. The U.S. EPA has modified a wide variety of items that require immediate response action for the first step of cleanup activities to be taken at the Wrigley Charcoal Site. The major goal of these cleanup activities is to address the most serious threats at the Wrigley Charcoal Site by removing contaminated media from the Primary Site flood plain, remediating wastes at the Storage Basin, and through limited access restrictions at the Primary Site and the Storage Basin. The cleanup activities as presented in this IRA Record of Decision (ROD) Amendment will achieve significant risk reduction and will prepare the Site for future remedial activities.

  5. 75 FR 55977 - Adequacy Status of the Knoxville, TN 1997 8-Hour Ozone Maintenance Plan Motor Vehicle Emission...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-15

    ...Blount, Jefferson, Knox, Sevier, and Loudon as well as the portion of Cocke County that falls within the boundaries of the Great Smoky Mountains National Park. On March 2, 1999, the United States Court of Appeals for the District of Columbia...

  6. A Case Study of the Cocke County (TN) School System and Its Role as a Partner in the NSF-Supported Appalachian Rural Systemic Initiative.

    ERIC Educational Resources Information Center

    Horn, Jerry G.; Oliver, Steve; Stufflebeam, Daniel

    This case study examines the history and current circumstances of education in Cocke County (Tennessee) in the context of its participation in the Appalachian Rural Systemic Initiative (ARSI), which aims to improve science and mathematics achievement through systemic reform. Sections of this report describe the county's history, demography, and…

  7. New data on the late Pleistocene history of lake fluctuations in the Sevier Desert, Utah

    NASA Astrophysics Data System (ADS)

    Oviatt, C. G.; Spencer, J. Q.; Fan, Y.; Leggett, A.

    2010-12-01

    The chronology of lake-level changes during the late Pleistocene in the Lake Bonneville basin, Utah, is currently understood in a general way. Because the regressive phase of the lake occurred during a critical time in the history of the basin (climate was changing rapidly, humans were moving into suitable habitats that were being made available by the decline of the large lake), it is important to improve the dating of paleohydrologic and paleoclimatic events. We have initiated field and laboratory studies in a part of the Sevier Desert that was studied at a reconnaissance scale 25 to 30 years ago. Our work consists of describing critical stratigraphic sections in natural exposures along the Sevier River and in a core of lake and fluvial sediments, and in dating suitable materials from these sections using radiocarbon and optically stimulated luminescence (OSL). The Sevier basin was integrated with the rest of Lake Bonneville during the time when its highest shorelines were being formed, including during the long period of development of the Provo shoreline (~17.6-14.6 cal ka). The lake regressed rapidly from the Provo shoreline (~1465 m) to the altitude of the divide between the Sevier basin and the Great Salt Lake (GSL) basin (~1390 m), then split into two lakes. In the GSL basin the lake continued to decline as a closed-basin lake, and the lake in the shallow Sevier basin overflowed into it along a large river (the ancestral Sevier River). An extensive wetland, fed by runoff from the Sevier and Beaver Rivers, and by groundwater discharge, developed over much of the Sevier Desert floor during the early Holocene. We have measured new OSL ages that compare favorably with previously obtained radiocarbon ages for the same stratigraphic units. Part of our work is aimed at evaluating the hypothesis that rapid draw-downs in lake level would cause increased groundwater discharge to the lake, and therefore a potential radiocarbon reservoir effect that would have been spatially and temporally heterogeneous.

  8. 36. View of southern terminus of the Blue Ridge Parkway ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. View of southern terminus of the Blue Ridge Parkway looking SW. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

  9. Inventory of wetlands and agricultural land cover in the upper Sevier River Basin, Utah

    NASA Technical Reports Server (NTRS)

    Jaynes, R. A.; Clark, L. D., Jr.; Landgraf, K. F. (principal investigator)

    1981-01-01

    The use of color infrared aerial photography in the mapping of agricultural land use and wetlands in the Sevier River Basin of south central utah is described. The efficiency and cost effectiveness of utilizing LANDSAT multispectral scanner digital data to augment photographic interpretations are discussed. Transparent overlays for 27 quadrangles showing delineations of wetlands and agricultural land cover were produced. A table summarizing the acreage represented by each class on each quadrangle overlay is provided.

  10. Paired, facing monoclines in the Sanpete-Sevier Valley area, central Utah

    USGS Publications Warehouse

    Witkind, I.J.

    1992-01-01

    Several major monoclines that trend northward through the Sanpete-Sevier Valley area of central Utah are paired and face one another. This pairing of monoclines may have occurred when near-horizontal sedimentary and volcanic strata subsided into voids created as salt was removed from a salt diapir concealed beneath valley fill. Removal was mostly by dissolution or extrusion during Neogene time. The paired monoclines, thus, are viewed as collapse features rather than as normal synclinal folds. -from Author

  11. 77 FR 17408 - Foreign-Trade Zone 204-Tri-Cities Area, TN/VA; Application for Reorganization Under Alternative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ...Business and Technology Park, 4509 West Stone Drive, Kingsport, Hawkins County, TN; Site 9 (134 acres, expires 6/30/15...service area under the ASF would be the Counties of Sullivan, Hawkins, Greene, Washington, Unicoi, Carter, Hamblen, and...

  12. Preliminary Feasibility Assessment of Geologic Carbon Sequestration Potential for TVA's John Sevier and Kingston Power Plants

    SciTech Connect

    Smith, Ellen D [ORNL; Saulsbury, Bo [ORNL

    2008-03-01

    This is a preliminary assessment of the potential for geologic carbon sequestration for the Tennessee Valley Authority's (TVA) John Sevier and Kingston power plants. The purpose of this assessment is to make a 'first cut' determination of whether there is sufficient potential for geologic carbon sequestration within 200 miles of the plants for TVA and Oak Ridge National Laboratory (ORNL) to proceed with a joint proposal for a larger project with a strong carbon management element. This assessment does not consider alternative technologies for carbon capture, but assumes the existence of a segregated CO{sub 2} stream suitable for sequestration.

  13. Cretaceous crustal structure and metamorphism in the hinterland of the Sevier thrust belt, western US Cordillera

    SciTech Connect

    Miller, E.L.; Gans, P.B. (Stanford Univ., CA (USA))

    1989-01-01

    Combined structural and geochronologic studies to the west of the Sevier belt at lat 39{degree}-40{degree}N indicate that peak metamorphism of the upper 15 km of crust occurred during the Late Cretaceous (70-90 Ma), overlapping in time with the intrusion of muscovite-bearing granites and pegmatites. Metamorphism was accompanied by penetrative deformation (top-to-the-east layer-parallel shear); both were localized at the currently exposed structural levels, resulting in impressive vertical and lateral strain gradients and T{sub max} gradients of > 50 C/km. Inception of thrust faulting in the Sevier belt may have predated peak metamorphism and intrusion of granites in the hinterland by 10-15 m.y., but it continued during and ended simultaneously with the end of metamorphism at about 70 Ma. The rise of muscovite-bearing granites and hot fluids, generated by metamorphism and crustal anatexis at greater depth, provides and explanation for the observed localized metamorphism and deformation at relatively high structural levels.

  14. Relationship of Cretaceous synorogenic conglomerate in central Utah to Sevier thrust events

    SciTech Connect

    Pratt, R.M.

    1985-05-01

    A boulder conglomerate facies is found almost continuously along the western edge of the Cretaceous seaway in central and southern Utah. At the present time, north-south continuity of the facies is disrupted by Tertiary volcanic cover and basin and range-type faulting, making the original distribution of conglomerate difficult to follow. Careful physical comparison of good conglomerate exposures and developing palynologic control in fine-grained equivalent sediments to the east allows some correlation of the facies trend and indicates a close time relationship with Sevier thrust events. Silicic and carbonate boulder conglomerates were limited to a relatively narrow east-west zone directly in front of the leading thrust edge. Rapid eastward transition to well-sorted sand and coastal marine conditions suggests deposition relatively close to base level. Major unconformities within the conglomerate sequence and the upper and lower limits of coarse clastic sedimentation define several depositional episodes. It appears that the conglomerate packages are related to specific Sevier orogenic events.

  15. The Feasibility of Enhacing Streamflow in the Sevier River Basin of Utah by Seeding Winter Mountain Clouds

    Microsoft Academic Search

    Arlin B. Super; David W. Reynolds

    1991-01-01

    The Bureau of Reclamation conducted a feasibility study of seeding winter mountain clouds as a possible means of enhancing the water supplies of the Sevier River Basin of Utah. A considerable body of pertinent meteorological observations from in or near the basin is examined. The evidence indicates that Utah winter mountain clouds often have more supercooled liquid water available than

  16. Geological control of springs and seeps in the Farmington Canyon Complex, Davis County, Utah

    E-print Network

    Skelton, Robyn Kaye

    1991-01-01

    County, Utah is located in the zone dividing the Basin and Range Province from the Colorado Plateau and Rocky Mountains. . . . . . 1 1 Figure 5. Location of study area in Davis County, Utah. . . . Figure 6. Geologic map of the Precambrian Farmington... Canyon Complex Figure 7. Six phases of the geologic evolution of Utah. . . . . 12 . . . 1 3 . . . 1 6 Figure 8. Fold axes associated with Sevier/Laramide compression are distorted by the existing uplifts. FCC = Farmington Canyon Complex...

  17. 14. Cades Cove Road, mountain view with single tree. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Cades Cove Road, mountain view with single tree. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  18. 23. Henry Whitehead place in Cades Cove looking ESE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Henry Whitehead place in Cades Cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  19. 28. Ford near the end of Cades Cove Loop Road ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Ford near the end of Cades Cove Loop Road looking NNW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  20. 25. Dan Lawson place in Cades Cove looking S. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. Dan Lawson place in Cades Cove looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  1. 9. Double arch culvert on Laurel Creek Road looking ENE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Double arch culvert on Laurel Creek Road looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  2. 4. Ford near the northern end of road looking NNE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Ford near the northern end of road looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  3. 19. Cantilevered barn in Cades Cove looking SSW. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Cantilevered barn in Cades Cove looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  4. 27. Abandoned treelined road in Cades Cove looking N. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. Abandoned tree-lined road in Cades Cove looking N. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  5. 7. Elevation of single arch stone bridge on Laurel Creek ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Elevation of single arch stone bridge on Laurel Creek Road looking N. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  6. 15. Cades Cove Road, mountain view with horses. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Cades Cove Road, mountain view with horses. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  7. 5. Laurel Creek Road, revetment wall and creek. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Laurel Creek Road, revetment wall and creek. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  8. 10. Concrete bridge on road to Tremont looking S. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Concrete bridge on road to Tremont looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  9. 2. Switchback and General Road view looking S. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Switchback and General Road view looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  10. 24. View of barn, fields and Cades Cove loop road ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. View of barn, fields and Cades Cove loop road looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  11. 21. View down Hyatt Lane in Cades Cove looking NNE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View down Hyatt Lane in Cades Cove looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  12. 1. View of Laurel Creek Road, revetment wall and cliff ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Laurel Creek Road, revetment wall and cliff looking S. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  13. 26. View of Cades Cove Loop Road with fields and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. View of Cades Cove Loop Road with fields and mountains looking NE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  14. 17. Methodist Church in Cades Cove looking NW. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Methodist Church in Cades Cove looking NW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  15. 18. View of fields, mountains and mist in Cades Cove ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of fields, mountains and mist in Cades Cove looking SW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  16. 12. Cades Cove Road, view toward entrance with ruts in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Cades Cove Road, view toward entrance with ruts in road. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  17. 16. First Baptist Church in Cades Cove looking SSW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. First Baptist Church in Cades Cove looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  18. 22. Cades Cove Valley from Hyatt Lane looking E. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Cades Cove Valley from Hyatt Lane looking E. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  19. 2. View of west tunnel portal and stream on Laurel ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View of west tunnel portal and stream on Laurel Creek Road looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  20. 8. Double arch culvert on Laurel Creek Road looking WSW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Double arch culvert on Laurel Creek Road looking WSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  1. 6. 1930's revetment wall next to 1886 constructed revetment wall ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. 1930's revetment wall next to 1886 constructed revetment wall on Laurel Creek Road looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  2. 13. Cades Cove Road, with barbed wire fence and mountain ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Cades Cove Road, with barbed wire fence and mountain view. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  3. 1. View of Cades Cove Valley from first overlook on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Cades Cove Valley from first overlook on Rich Mountain Road looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  4. 7. Placed boulder revetment wall, river and roadway looking SSW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Placed boulder revetment wall, river and roadway looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Heintooga Round Bottom Road & Balsam Mountain Road, Between Blue Ridge Parkway & Big Cove Road, Gatlinburg, Sevier County, TN

  5. 5. Ford near western end of road looking NNW. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Ford near western end of road looking NNW. - Great Smoky Mountains National Park Roads & Bridges, Heintooga Round Bottom Road & Balsam Mountain Road, Between Blue Ridge Parkway & Big Cove Road, Gatlinburg, Sevier County, TN

  6. 1. Entrance to Heintooga Round Bottom Road and sign looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Entrance to Heintooga Round Bottom Road and sign looking N. - Great Smoky Mountains National Park Roads & Bridges, Heintooga Round Bottom Road & Balsam Mountain Road, Between Blue Ridge Parkway & Big Cove Road, Gatlinburg, Sevier County, TN

  7. 4. General view of road with grey birch trees looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. General view of road with grey birch trees looking E. - Great Smoky Mountains National Park Roads & Bridges, Heintooga Round Bottom Road & Balsam Mountain Road, Between Blue Ridge Parkway & Big Cove Road, Gatlinburg, Sevier County, TN

  8. 2. General Road view looking SW. Great Smoky Mountains ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. General Road view looking SW. - Great Smoky Mountains National Park Roads & Bridges, Heintooga Round Bottom Road & Balsam Mountain Road, Between Blue Ridge Parkway & Big Cove Road, Gatlinburg, Sevier County, TN

  9. 6. Horse camp looking W. Great Smoky Mountains National ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Horse camp looking W. - Great Smoky Mountains National Park Roads & Bridges, Heintooga Round Bottom Road & Balsam Mountain Road, Between Blue Ridge Parkway & Big Cove Road, Gatlinburg, Sevier County, TN

  10. 3. View of overlook and vista looking SW. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. View of overlook and vista looking SW. - Great Smoky Mountains National Park Roads & Bridges, Heintooga Round Bottom Road & Balsam Mountain Road, Between Blue Ridge Parkway & Big Cove Road, Gatlinburg, Sevier County, TN

  11. 2. Big Creek Road, worm fence and road at trailhead. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Big Creek Road, worm fence and road at trailhead. - Great Smoky Mountains National Park Roads & Bridges, Big Creek Road, Between State Route 284 & Big Creek Campground, Gatlinburg, Sevier County, TN

  12. 10. Area of landslide on eastern section of Foothills Parkway ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Area of landslide on eastern section of Foothills Parkway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

  13. 10. Reagan's Tub Mill and Roadway looking NE. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Reagan's Tub Mill and Roadway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  14. 11. Roaring Fork Motor Nature Trail, boulders along road after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  15. 3. Roaring Fork Motor Nature Trail, view between second and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  16. 9. Roaring Fork Motor Nature Trail, Reagan House. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  17. 12. Roaring Fork Motor Nature Trail, place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  18. 6. Roaring Fork Motor Nature Trail, road view after stop ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  19. Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  20. 1. Roaring Fork Motor Nature Trail, entrance sign. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  1. 2. Roaring Fork Motor Nature Trail, road view before first ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  2. 8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  3. 4. Overgrown overlook number 1 looking W. Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Overgrown overlook number 1 looking W. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  4. 7. Roaring Fork Motor Nature Trail, rocks along edge of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  5. 5. Roaring Fork Motor Nature Trail, vista at stop three. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  6. 77 FR 21505 - Proposed Amendment of Class D and E Airspace; Blountville, TN, and Revocation of Class E Airspace...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-10

    ...deg]02'00'' W.) Rogersville, Hawkins County Airport, TN (Lat. 36[deg...Airport and within a 7-mile radius of Hawkins County Airport, and within 7 miles each...the 7-mile radius to 12 miles east of Hawkins County Airport. Issued in College...

  7. Role of crustal thickening and extensional collapse in the tectonic evolution of the Sevier-Laramide orogeny, western United States

    SciTech Connect

    Livaccari, R.F. (Univ. of New Mexico, Albuquerque (United States))

    1991-11-01

    The effects of crustal thickening and extensional collapse of continental crust, combined with variations in plate-convergence forces, provide a coherent explanation for evolution of the Cretaceous-early Tertiary Sevier-Laramide orogeny. Crustal thickening of the Sevier-Laramide hinterland region was triggered by the combination of compressive plate-convergence forces and subduction-induced conductive heating of the crust. Eastward progradation of the locus of Sevier-Laramide deformation through time reflects the progressive widening of the region of crustal thickening. The region of maximum crustal thickening migrated outward until about 80 to 75 Ma, when it encountered excessively strong lithosphere of the Colorado Plateau. The locus of deformation was then transmitted laterally across both a hinterland that had attained maximum crustal thickness and the rigid Colorado Plateau, into the Laramide Rocky Mountain foreland. An episode of vigorous extensional collapse of orogenically thickened crust affected the southern Cordillera between about 75 and 35 Ma. Early Tertiary waning of plate-convergence rates and continued subduction-induced conductive heating of thickened crust allowed extensional collapse along the southern Cordillera region to drive the Colorado Plateau block northward. The result was the late Laramide phase of Rocky Mountain foreland deformation in the central and southern Laramide Rocky Mountain region continued through Eocene time, rather than ceasing at 56 Ma, as it did in the northern Cordillera.

  8. 3. BIG HOUSE (left) AND CORN CRIB (right) IN THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. BIG HOUSE (left) AND CORN CRIB (right) IN THE BACKGROUND. See also individual HABS documentation: Walker Family Farm, Big House (HABS No. TN-121 A), and Walker Family Farm, Corn Crib (HABS No. TN-121 C). - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

  9. Immunochemical studies on Tn erythrocyte glycoprotein.

    PubMed

    Lee, L T; Frank, S; de Jongh, D S; Howe, C

    1981-12-01

    Glycoproteins were extracted from membranes of erythrocytes that displayed Tn polyagglutination and were compared chemically and immunologically with glycoproteins of group O, MN cells. Tn glycoprotein had lower than normal NANA : protein and sugar : protein ratios, as revealed by direct analysis and polyacrylamide gel electrophoresis, and displayed slower immunoelectrophoretic mobility than glycoproteins of group O, MN cells. Agglutination of Tn cells by Salvia sclarea lectin was inhibited by Tn glycoprotein but not by O, MN glycoprotein. Tn and MN glycoproteins were equally potent inhibitors of influenza virus HA. Our findings indicate than Tn-specific determinants are part of the glycophorin molecule. PMID:6171313

  10. August 13, 2010 Knoxville, TN

    E-print Network

    as a UTC with a strategic theme of Heavy Vehicle Safety, Security and Operational Efficiency · 2010Truck® Overview4 #12;· Knoxville, TN where UT operates the Trusted Truck Management Center · The truck being inspected will also be in Knoxville · Greensboro, NC where Volvo operates the Fleet Operations Center 13 Aug

  11. Hydrologic evaluation and water-supply considerations for five Paiute Indian land parcels, Millard, Sevier, and Iron counties, southwestern Utah

    USGS Publications Warehouse

    Price, Don; Stephens, D.W.; Conroy, L.S.

    1989-01-01

    The hydrologic resources in and adjacent to five parcels of land held in trust for the Paiute Indian Tribe of Utah were evaluated. The land, located in southwestern Utah, is generally arid and has had only limited use for grazing. The parcels are located near the towns of Cove Fort, Joseph, Koosharem, and Kanarraville. On the basis of available geohydrologic and hydrologic data, water of suitable quality is locally available in the areas of all parcels for domestic, stock, recreation, and limited irrigation use. Developing this water for use on the parcels would potentially involve obtaining water rights, drilling wells, and constructing diversion structures. Surface water apparently is the most favorable source of supply available for the Joseph parcel, and groundwater apparently is the most favorable source of supply available for the other parcels. (USGS)

  12. Yavapai County Maricopa County

    E-print Network

    Laughlin, Robert B.

    AZ 10 60 93 89 60 Alamo Lake 72 71 89 89 Yavapai County Maricopa County Yuma County Mohave County River National Wildlife Refuge Kofa National Wildlife Refuge State Line County Boundary Solar Energy Analyzed for Solar Development in PEIS (As of 6/5/2009) Surface Management Agency As of 3/26/2009 Tribal

  13. Inhibition of TnI-TnC interaction and contraction of skinned muscle fibres by the synthetic peptide TnI [104–115

    Microsoft Academic Search

    J. C. Riiegg; C. Zeugner; J. Van Eyk; C. M. Kay; R. S. Hodges

    1989-01-01

    Circular dichroism was used to study the induction of helix in TnC or TnI-TnC by the TnI peptide [104–115] at various Ca2+ concentrations. The increase in negative ellipticity and pCa2+ values for the peptide-TnC complex, indicates that binding of the peptide to TnC, induces a small helical conformational change in TnC. This results in an increase in the Ca2+ binding

  14. Presence of the Tn antigen on hematopoietic progenitors from patients with the Tn syndrome.

    PubMed Central

    Vainchenker, W; Vinci, G; Testa, U; Henri, A; Tabilio, A; Fache, M P; Rochant, H; Cartron, J P

    1985-01-01

    The Tn syndrome is an acquired clonal disorder characterized by the exposure of a normally hidden determinant, the Tn antigen, on the surface of human erythrocytes, platelets, granulocytes, and lymphocytes. Two distinct populations, Tn positive (Tn+) and Tn negative (Tn-), of mature hemopoietic cells are present in Tn patients. To determine whether the Tn antigen is already expressed on erythroid, myeloid, and pluripotent progenitors, light-density mononuclear blood cells from two patients with this syndrome were separated by fluorescent-activated cell sorting and by affinity chromatography into Tn+ and Tn- fractions, using their binding properties to Helix pomatia agglutinin (HPA). Burst-forming-unit erythroid (BFU-E), colony-forming-unit granulocyte/macrophage (CFU-GM), cells were assayed in plasma clot cultures. After 12-14 d of culture, colonies were studied by a double fluorescent labeling procedure. First, a fluorescein-conjugated HPA permitted evaluation of the presence or absence of the Tn antigen at the surface of the cells composing each colony, and second, the binding of a murine monoclonal antibody against either glycophorin A (LICR-LON-R10) or against a myeloid antigen (80H5), revealed by an indirect fluorescent procedure, was used to establish the erythroid or myeloid origin of each cell. The Tn+ fraction obtained by cell sorting gave rise to nearly 100% Tn+ colonies composed exclusively of cells bearing this antigen. The reverse was observed for the Tn- cell fraction. These results demonstrate that in the Tn syndrome, BFU-E, CFU-GM, and CFU-GEMM of the Tn+ clone express the Tn antigen at this early stage of differentiation. Images PMID:3973016

  15. Structural styles of the Guess Creek fault block beneath the Great Smoky thrust sheet, Blount County, Tennessee

    SciTech Connect

    Carter, M.W.; Davidson, G.L.; Heller, J.A. (Univ. of Tennessee, Knoxville, TN (United States). Dept. of Geological Sciences); Hatcher, R.D. Jr. (Oak Ridge National Lab., TN (United States))

    1993-03-01

    A road cut along US 321 N, approximately 1 km NW of Walland, TN, exposes a previously unexposed complexly deformed section of Middle Ordovician clastic wedge [Chickamauga Group, Sevier Shale] sedimentary rocks. It provides an excellent opportunity to analyze both the lithologic assemblages and complex folding and faulting beneath the Great Smoky thrust sheet. Arkosic quartzite of the Lower Cambrian Cochran Conglomerate [Chilhowee Group], has been thrust over weaker Sevier Shale in the hanging wall of the Guess Creek fault. Regionally, the Great Smoky fault separates metamorphosed Precambrian to Lower Cambrian clastic shelf, slope, and rift facies rocks of the western Blue Ridge from Cambro-Ordovician carbonate shelf and orogenic wedge deposits of the foreland fold and thrust belt. West of the Great Smoky fault, the Guess Creek fault has been interpreted to floor duplexed Cambro-Ordovician rocks exposed in windows beneath the Great Smoky thrust sheet in the vicinity of the Great Smoky Mountains National Park. The Sevier Shale here consists of variably cleaved shale, siltstone, sandstone, and conglomerate. It exhibits a variety of fold styles throughout the exposure, ranging from predominantly noncylindrical tight folds to broad, open structures. A weak axial-planar pencil cleavage is developed in the Middle Ordovician shale and siltstone, along with a secondary cleavage that transects the axial surfaces of the folds. Minor thrust faults within the Sevier Shale appear to have formed by propagation through tightened fold hinges or bedding-parallel slip. The fold pattern observed in the roadcut appears to be partly the result of movement along a tear fault that broke both the hanging wall and footwall of the Great Smoky thrust sheet after emplacement. Slickenline orientations along minor thrust surfaces in the Cochran Conglomerate indicate eastward-directed, oblique-slip movement of the tear fault.

  16. Protein glycosylation: chaperone mutation in Tn syndrome.

    PubMed

    Ju, Tongzhong; Cummings, Richard D

    2005-10-27

    Tn syndrome is a rare autoimmune disease in which subpopulations of blood cells in all lineages carry an incompletely glycosylated membrane glycoprotein, known as the Tn antigen. This truncated antigen has the sugar N-acetylgalactosamine alpha-linked to either a serine or threonine amino-acid residue, whereas the correct T antigen has an additional terminal galactose; the defect may be due to a malfunction of the glycosylating enzyme T-synthase. Here we show that Tn syndrome is associated with a somatic mutation in Cosmc, a gene on the X chromosome that encodes a molecular 'chaperone' that is required for the proper folding and hence full activity of T-synthase. The production of the autoimmune Tn antigen by a glycosyltransferase enzyme rendered defective by a disabled chaperone may have implications for other Tn-related disorders such as IgA nephropathy, a condition that can result in renal failure. PMID:16251947

  17. Layer-parallel shortening across the Sevier fold-thrust belt and Laramide foreland of Wyoming: spatial and temporal evolution of a complex geodynamic system

    NASA Astrophysics Data System (ADS)

    Weil, Arlo Brandon; Yonkee, W. Adolph

    2012-12-01

    Varying patterns of layer-parallel shortening (LPS) and vertical-axis rotations from the thin-skin Sevier fold-thrust belt to the thick-skin Laramide foreland of Wyoming are quantified from integrated structural, anisotropy of magnetic susceptibility (AMS), and paleomagnetic analyses. Within the Sevier belt, widespread early LPS was accommodated by spaced cleavage, fracture sets, minor folds, and minor faults. LPS directions are subperpendicular to structural trends of systematically curved thrust sheets of the Wyoming salient, reflecting a combination of primary dispersion and secondary rotation during thrusting. Within the Laramide foreland, limited LPS was accommodated mostly by minor faults with conjugate wedge and strike-slip geometries. LPS directions in gentler fold limbs vary from perpendicular to acute with structural trends of variably oriented, anastomosing basement-cored arches. Steep forelimbs display more complex relations, including younger fault sets that developed during evolving stress states and localized vertical-axis rotations. Although internal strain is limited, weak AMS lineations defined by kinked and rotated phyllosilicates are widely developed and consistently oriented perpendicular to measured LPS directions. Palinspastically restored LPS directions, corrected for paleomagnetically determined vertical-axis rotations, vary on average from W-E in the Sevier belt to WSW-ENE in the Laramide foreland. In detail, LPS directions display deflections related to primary sedimentary wedge geometry and basement fabrics. LPS in the Sevier belt is interpreted to partly reflect stress transmitted from the hinterland through the growing orogenic wedge and topographic stress along the front of the wedge. LPS in the Laramide foreland is interpreted to partly reflect basal traction during flat-slab subduction beneath thick cratonic lithosphere, with spatial-temporal variations in stress trajectories related to basement heterogeneities and evolving fault systems. Evidence for significant late N-S Laramide shortening, as proposed by multi-stage shortening models, is not observed.

  18. Thyroid hormone inhibits slow skeletal TnI expression in cardiac TnI-null myocardial cells

    Microsoft Academic Search

    Beth Riedel; Yuanyuan Jia; Jianfeng Du; Sarah Akerman; Xupei Huang

    2005-01-01

    A cardiac troponin I (cTnI) gene knockout mouse model has been created and the phenotype of the cTnI null mice is an acute heart failure resulting from the deficiency of TnI and a diastolic dysfunction. Two isoforms of TnI (the fetal form ssTnI and the adult form cTnI) are mainly expressed in the heart under a developmentally regulated program. In

  19. 75 FR 26814 - Tennessee Disaster #TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ...26814] [FR Doc No: 2010-11191... 12161 and 12162] Tennessee Disaster TN-00038...Only for the State of Tennessee (FEMA- 1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding, Straight-Line...

  20. Protein glycosylation: Chaperone mutation in Tn syndrome

    Microsoft Academic Search

    Tongzhong Ju; Richard D. Cummings

    2005-01-01

    Tn syndrome is a rare autoimmune disease in which subpopulations of blood cells in all lineages carry an incompletely glycosylated membrane glycoprotein, known as the Tn antigen. This truncated antigen has the sugar N-acetylgalactosamine alpha-linked to either a serine or threonine amino-acid residue, whereas the correct T antigen has an additional terminal galactose; the defect may be due to a

  1. Tn polyagglutinability occurring in a patient with B cell lymphoma

    Microsoft Academic Search

    M. Wallner; R. Waldner

    1985-01-01

    A case of polymorphic immunocytoma (B cell lymphoma) coinciding with expression of Tn antigen on a population of erythrocytes is presented. Tn activation was found incidentally by screening blood samples of patients suffering from hematologic malignancies with a Tn specific lectin from Salvia sclarea. So far, Tn activation has been reported only in apparently healthy subjects or in subjects suffering

  2. Tn polyagglutinability occurring in a patient with B cell lymphoma.

    PubMed

    Wallner, M; Waldner, R

    1985-11-01

    A case of polymorphic immunocytoma (B cell lymphoma) coinciding with expression of Tn antigen on a population of erythrocytes is presented. Tn activation was found incidentally by screening blood samples of patients suffering from hematologic malignancies with a Tn specific lectin from Salvia sclarea. So far, Tn activation has been reported only in apparently healthy subjects or in subjects suffering from or developing myeloid leukemia. PMID:3933597

  3. 14. Roaring Fork Motor Nature Trail, Place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  4. ATP-dependent specific binding of Tn3 transposase to Tn3 inverted repeats

    NASA Astrophysics Data System (ADS)

    Wishart, W. L.; Broach, J. R.; Ohtsubo, E.

    1985-04-01

    Transposons are discrete segments of DNA which are capable of moving from one site in a genome to many different sites1,2. Tn3 is a prokaryotic transposon which is 4,957 base pairs (bp) long and encodes a transposase protein which is essential for transposition3-7. We report here a simple method for purifying Tn3 transposase and demonstrate that the transposase protein binds specifically to the ends of the Tn3 transposon in an ATP-dependent manner. The transposase protein binds to linear double-stranded DNA both nonspecifically and specifically; the nonspecific DNA binding activity is sensitive to challenge with heparin. Site-specific DNA binding to the ends (inverted repeats) of Tn3 is observed only when binding is performed in the presence of ATP; this ATP-dependent site-specific DNA binding activity is resistant to heparin challenge. Our results indicate that ATP qualitatively alters the DNA binding activity of the transposase protein so that the protein is able to bind specifically to the ends of the Tn3 transposon.

  5. Superfund Record of Decision (EPA Region 4): Milan Army Ammunition Plant, Milan, TN, March 11, 1998

    SciTech Connect

    NONE

    1998-09-01

    This decision document presents the selected action for the Salvage Yard, Former Ammunition Burnout Area (ABA), and Sanitary Landfill at MLAAP, located in Gibson and Carroll Counties, TN. This ROD addresses the final response action planned for the Salvage Yard, Former ABA, and Sanitary Landfill, including soil and groundwater. NFA is the selected remedy for soil and groundwater at the Salvage Yard, Former ABA, and Sanitary landfill. The selected remedy manages the risk to acceptable levels for both human health and the environment and is the final action planned.

  6. 76 FR 29284 - Tennessee Disaster #TN-00055

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ...disaster: Primary Counties: Benton, Carroll, Crockett, Dyer, Gibson, Henderson, Henry, Houston, Lake, Lauderdale, Madison...Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster...

  7. Summary of reported agriculture and irrigation water use in southwestern Arkansas counties, 1991

    USGS Publications Warehouse

    Holland, T.W.; Manning, C.A.; Stafford, K.L.

    1993-01-01

    This report summarizes the 1991 water-use reporting through the Conservation District Offices in the following southwestern Arkansas counties: Bradley, Calhoun, Clark, Cleveland, Columbia, Dallas, Garland, Grant, Hempstead, Hot Spring, Howard, Little River, Montgomery, Nevada, Ouachita, Pike, Polk, Saline, Sevier, and Union. The number of withdrawal registrations for southwestern Arkansas counties was 132 (31 groundwater and 101 surface water). Water withdrawals reported during the registration process total 0.84 Mgal/d (none from groundwater and 0.84 Mgal/d surface water) for agriculture and 14.22 Mgal/d (1.64 Mgal/d groundwater and 12.58 Mgal/d surface water) for irrigation. The registration reports for 1991 indicate that this water was applied to 8,455 acres of land to irrigate rice, corn, sorghum, soybeans, cotton, cash grains, vegetables, sod, berries, fruit trees, timber, shrubs, and nuts as well as for the agricultural use of animal aquaculture.

  8. Diversity of putative Tn5253-like elements in Streptococcus pneumoniae.

    PubMed

    Henderson-Begg, S K; Roberts, A P; Hall, L M C

    2009-04-01

    Pneumococcal resistance to tetracycline, chloramphenicol, erythromycin and clindamycin is often attributed to carriage of conjugative transposons of the Tn916 family. The less well studied conjugative transposon Tn5253 is a composite transposon consisting of a Tn916-like element inserted within the unrelated Tn5252 element, which has also been associated with chloramphenicol and tetracycline resistance. Here, carriage of the Tn5252 integrase (int(5252)), Tn5252-encoded umuC and umuD homologues and Tn916 integrase (int(916)) was examined among 55 clinical isolates of Streptococcus pneumoniae resistant to one or more of the above mentioned antibiotics. Tn5253-associated genes were common among the antibiotic-resistant S. pneumoniae examined, including members of international clones, although the spectrum of genes and resistances carried was diverse. Analysis of five isolates demonstrated insertion of a Tn5253-related element at the same chromosomal locus but sequence and restriction site diversity. This study shows for the first time a high degree of variability of Tn5253-related elements within clinical isolates of pneumococci. The fact that these elements are prevalent among internationally recognised pandemic clones warrants a more intensive investigation. PMID:19097761

  9. Expression of the fast twitch troponin complex, fTnT, fTnI and fTnC, in vascular smooth muscle

    PubMed Central

    Moran, Carlos M.; Garriock, Robert J.; Miller, Melanie K.; Heimark, Ronald L.; Mudry, Ryan E.; Gregorio, Carol C.; Krieg, Paul A.

    2008-01-01

    It is generally believed that proteins of the troponin complex are not expressed in smooth muscle. We have directly assayed for expression of troponin transcripts in mouse vascular smooth muscle and found that troponin sequences normally associated with fast twitch skeletal muscle (fTnT, fTnI, fTnC) were present at significant levels in the thoracic aorta. In situ hybridization experiments demonstrated that fTnT, fTnI and fTnC transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes. Protein blot analysis using rat tissue showed that at least two members of the troponin complex, Troponin T and Troponin I, were translated in vascular smooth muscle of the aorta. Finally, immuno-fluorescence microscopy of rat aortic smooth muscle revealed that TnT and TnI are localized in a unique pattern, coincident with the distribution of tropomyosin. It seems likely therefore, that a complete troponin complex is expressed in vascular smooth muscle and is associated with the contractile machinery of the cell. These observations raise the possibility that troponins play a role in regulation of smooth muscle function. PMID:18548613

  10. Combining paleomagnetism and structural geology to better understand complex orogenic systems: development of a robust kinematic model for Sevier fold-thrust belt and Laramide foreland of Wyoming, U.S.A.

    NASA Astrophysics Data System (ADS)

    Weil, Arlo; Yonkee, Adolph

    2013-04-01

    Varying patterns of layer-parallel shortening (LPS) and vertical-axis rotations from the thin-skin Sevier fold-thrust belt to the thick-skin Laramide foreland of Wyoming are quantified from integrated structural, anisotropy of magnetic susceptibility (AMS), and paleomagnetic analyses. Within the Sevier belt, widespread early LPS was accommodated by spaced cleavage, fracture sets, minor folds, and minor faults. LPS directions are subperpendicular to structural trends of systematically curved thrust sheets of the Wyoming salient, reflecting a combination of primary dispersion and secondary rotation during thrusting. Within the Laramide foreland, limited LPS was accommodated mostly by minor faults with conjugate wedge and strike-slip geometries. LPS directions in gentler fold limbs vary from perpendicular to acute with structural trends of variably oriented, anastomosing basement-cored arches. Steep forelimbs display more complex relations, including younger fault sets that developed during evolving stress states and localized vertical-axis rotations. Although internal strain is limited, weak AMS lineations defined by kinked and rotated phyllosilicates are widely developed and consistently oriented perpendicular to measured LPS directions. Palinspastically restored LPS directions, corrected for paleomagnetically determined vertical-axis rotations, vary on average from W-E in the Sevier belt to WSW-ENE in the Laramide foreland. In detail, LPS directions display deflections related to primary sedimentary wedge geometry and basement fabrics. LPS in the Sevier belt is interpreted to partly reflect stress transmitted from the hinterland through the growing orogenic wedge and topographic stress along the front of the wedge. LPS in the Laramide foreland is interpreted to partly reflect basal traction during flat-slab subduction beneath thick cratonic lithosphere, with spatial-temporal variations in stress trajectories related to basement heterogeneities and evolving fault systems. Evidence for significant late N-S Laramide shortening, as proposed by multi-stage shortening models, is not observed.

  11. Transposable elements Updated March 29, 2012 4 (none) Tpm kan (Tn903) Tn903 pkan none none --K CDE Epicentre

    E-print Network

    Borenstein, Elhanan

    Transposable elements Updated March 29, 2012 Vector(s)d Reference 4 (none) Tpm kan (Tn903) Tn903 pkan none none - - K CDE Epicentre (none) Tpm tp (dhfr ) Tn7 pdhfr none none - - N/D N loxP 63-codon, HA, H6 - L2 ABC [5] T9 IS/hah pCM1008 Tpm strep/spec ? none loxP 63-codon, HA, H6 - N

  12. Characterization of Tn916S, a Tn916Like Element Containing the Tetracycline Resistance Determinant tet(S)

    Microsoft Academic Search

    Holli Lancaster; Adam P. Roberts; Raman Bedi; Michael Wilson; Peter Mullany

    2004-01-01

    Tetracycline-resistant streptococci are frequently isolated from the oral cavity of humans (13), and resistance is most commonly conferred by Tet(M), a ribosomal protection pro- tein often associated with the conjugative transposon (cTn) Tn916 (4). Tn916 belongs to a family of cTns that are com- posed of functional modules (10, 12) involved in conjugation, antibiotic resistance, regulation, and integration and excision.

  13. Magnitude, Timing, and Geometry of Extension in the Southern Sevier Desert Basin From Piercing Points, Seismic-Stratigraphic Reconstruction, and Deep well Data

    NASA Astrophysics Data System (ADS)

    Coogan, J. C.; Decelles, P. G.

    2007-12-01

    Palinspastic reconstruction of Mesozoic thrust sheets provides the main constraint for an estimated 47 km of Cenozoic extensional displacement along the Sevier Desert detachment (SDD) in the central Sevier Desert Basin. Hanging wall and footwall piercing points indicate that the SDD accommodated a minimum of 35 km of extensional displacement in the narrower southern part of the basin. The piercing points for the SDD are defined by the intersection of the SDD, the Canyon Range thrust (CRT), and a regional early Cenozoic erosion surface (ES). The hanging wall piercing point lies immediately northeast of the Cricket Mountains, where the SDD-CRT- ES intersection is narrowly defined by intersecting structure maps derived from published seismic reflection data. The footwall piercing point lies in the southern foothills of the Canyon Range, where the SDD breakaway plane is well constrained by an industry seismic line that lies within 2 km of the exposed intersection of the CRT with the base of the Oligocene Oak City Formation. Timing of extension in the southern Sevier Desert basin is constrained by a kinematic reconstruction of detachment and imbricate fault displacement, footwall uplift, and supradetachment sedimentation for Oligocene, Miocene, and Plio-Pleistocene seismic sequences. The reconstruction is centered on a seismic reflection and gravity interpretation along the published Pan Canadian profiles 2 and 3 that is tied to dated intervals in six industry wells. Fault restoration indicates that Oligocene and Miocene phases of slip each accounted for about 40 percent of the total displacement. Simultaneous backstripping of the Oligocene, Miocene, and Plio-Pleistocene supradetachment sequences records hanging wall subsidence simultaneous with footwall uplift, with a footwall burial history that is consistent with published Miocene apatite and zircon fission-track ages of footwall samples. The geometric evolution of the southern SDD extensional system is consistent with its development above a broad westward-migrating "rolling hinge" zone associated with isostatic uplift of the detachment footwall. Hanging wall normal faults east of the footwall crest exhibit small post-Miocene displacement, with demonstrable Quaternary slip restricted to the crest and western limb of the uplift, most notably along the Black Rock and Clear Lake fault zones. Early abandonment of the eastern part of the detachment may explain the indistinct geomorphic and structural expression of the break-away zone at the surface. The deepest level of the southern SDD also presents a complex geometry and kinematic history. The 1996 Chevron 1-29 Black Rock Federal well through the western basin margin penetrated a normal fault that places Jurassic over lower Cambrian strata at 4650 m measured depth, well above the principal SDD seismic reflection. The fault is not correlated to any large- displacement high-angle fault at shallow levels, and may form the abandoned roof to an extensional duplex.

  14. From Source to Sink: Exhumation of the North America Cordillera Revealed by Multi-dating of Detrital Minerals from Upper Jurassic-Upper Cretaceous Sevier Foreland Basin

    NASA Astrophysics Data System (ADS)

    Painter, C. S.; Carrapa, B.; DeCelles, P. G.; Gehrels, G. E.; Thomson, S. N.

    2013-12-01

    We sampled twenty-two Late Jurassic to Late Cretaceous syn-orogenic conglomerate clasts in proximal units in the Sevier fold-thrust belt and their distal sandstone equivalents up to 300 km east of the thrust front, in Utah, Colorado, Wyoming, and South Dakota. To better constrain depositional ages, these samples were analyzed using detrital zircon U-Pb (DZ U-Pb) geochronology. To identify a thermochronometer that measures source exhumation in the North America Cordillera, both zircon (U-Th)/He and apatite fission track (AFT) thermochronology was utilized, on both the conglomerate cobbles and sandstone (detrital) samples. Eleven samples were analyzed with zircon (U-Th)/He; however, discordant ages in the conglomerate cobble samples suggest that this system was not fully reset and never experienced T> ~180 °C in the source stratigraphy during the Sevier orogeny. Eleven other samples are analyzed using apatite fission track thermochronology (AFT); AFT ages are generally similar or older than depositional ages indicating that the detrital ages record source exhumation signals, and that exhumation depth corresponds to T>~120 °C. In order to test whether or not the youngest cooling AFT age population represents a source exhumation signal or a co-magmatic signal we here performed double dating of the detrital AFT samples using apatite U-Pb thermochronology. Maximum depositional ages using DZ U-Pb match existing age controls on basin stratigraphy. Our study shows that AFT is an effective thermochronometer to detect source exhumation for Cretaceous foreland stratigraphy in the western U.S.A. Lag-times (i.e. the difference between the source exhumation age and depositional age) are ~0 to 5 Myr with relatively steady-state to slightly increasing exhumation rates suggesting orogenic growth at this time. The very short lag times also indicate limited to no storage time between source and sink. The AFT lag time of the Early Cretaceous Kelvin Formation is ~5 Myr and represents a time when the Canyon Range Thrust was active to the southwest. Upper Cretaceous units have an approximate lag time of 0 Myr, during a time when the Willard, Crawford, Medicine Butte and Absaroka Thrust systems were active to the west. These lag-time measurements correspond to exhumation rates of ~0.8 mm/yr to >>1 mm/yr.

  15. Tn2009, a Tn916Like Element Containing mef(E) in Streptococcus pneumoniae

    Microsoft Academic Search

    Maria Del Grosso; Anna Scotto d'Abusco; Francesco Iannelli; Gianni Pozzi; Annalisa Pantosti

    2004-01-01

    The association between the macrolide efflux gene mef(E) and the tet(M) gene was studied in two clinical strains of Streptococcus pneumoniae that belonged to serotypes 19F and 6A, respectively, and that were resistant to both tetracycline and erythromycin. The mef(E)-carrying element mega (macrolide efflux genetic assembly; 5,511 bp) was found to be inserted into a Tn916-like genetic element present in

  16. Tn2001, a transposon encoding chloramphenicol resistance in Pseudomonas aeruginosa.

    PubMed Central

    Iyobe, S; Sagai, H; Mitsuhashi, S

    1981-01-01

    We isolated a new transposon, Tn2001, from the group P-2 plasmid Rms159-1 in Pseudomonas aeruginosa. Tn2001-encoded chloramphenicol resistance did not result from the formation of chloramphenicol acetyltransferase. Tn2001 was transposable between temperate phages and conjugative and nonconjugative plasmids belonging to various incompatibility groups, including P-1, P-3, P-4, P-5, P-7, and P-8 in P. aeruginosa. Transposition occurred independently of the general recombination ability of the Pseudomonas host, and its frequency varied between 10(-1) and 10(-8), depending upon the donor and recipient replicons. Tn2001 transposition also occurred in a recombination-deficient strain of Escherichia coli. Agarose gel electrophoresis and electron microscopic observations revealed that Tn2001 could transpose to different sites in the RP4 replicon and that the transposed deoxyribonucleic acid fragment was 2.1 kilobases long. Images PMID:6260739

  17. Sedimentology and provenance of Late Cretaceous(. )-Eocene Sheep Pass Formation conglomerates in hinterland of Sevier Orogenic Belt, east-central Nevada

    SciTech Connect

    Diem, D.A.; Schmitt, J.G.

    1986-08-01

    The Late Cretaceous(.)-Eocene Sheep Pass Formation of east-central Nevada is a thick (1500-m) sequence of nonmarine conglomerate, sandstone, mud rock, and limestone. Detailed stratigraphic analyses of the basal conglomerate (50 to 450 m thick) in the Egan, Pancake, and Fish Creek ranges was performed. The presence of large (up to 400 m) Paleozoic limestone slide blocks and paucity of sandstone lithofacies within conglomeratic sequences in the Egan Range suggest deposition on the proximal to medial portion of streamflow-dominated alluvial fans. Clast imbrication measurements document east-northeast to west-southwest paleoflow directions throughout the region. In the Egan and Pancake Ranges, gravel is comprised predominantly of Pennsylvanian-Permian carbonate and Mississippian quartzarenite (Scotty Wash). Gravels in the Fish Creek Range consist of paleozoic dolomite and Ordovician quartzarenite (Eureka) at the base of the Sheep Pass, and Paleozoic limestone and dolomite, Mississippian quartzarenite, and Ordovician quartzarenite near the top. Preliminary interpretations of our data indicate that Late Cretaceous-early Tertiary sedimentation in the hinterland of the Sevier orogenic belt occurred in a series of small basins characterized by general westward sediment dispersal patterns. Further interpretations of hinterland basin development and structural setting of basin-bounding uplifts await further work on Cretaceous-Eocene strata now in progress.

  18. Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements.

    PubMed Central

    Rådström, P; Sköld, O; Swedberg, G; Flensburg, J; Roy, P H; Sundström, L

    1994-01-01

    Integrons confer on bacterial plasmids a capability of taking up antibiotic resistance genes by integrase-mediated recombination. We show here that integrons are situated on genetic elements flanked by 25-bp inverted repeats. The element carrying the integron of R751 has three segments conserved with similar elements in Tn21 and Tn5086. Several characteristics suggest that this element is a transposon, which we call Tn5090. Tn5090 was shown to contain an operon with three open reading frames, of which two, tniA and tniB, were predicted by amino acid similarity to code for transposition proteins. The product of tniA (559 amino acids) is a probable transposase with 25% amino acid sequence identity to TnsB from Tn7. Both of these polypeptides contain the D,D(35)E motif characteristic of a protein family made up of the retroviral and retrotransposon IN proteins and some bacterial transposases, such as those of Tn552 and of a range of insertion sequences. Like the transposase genes in Tn552, Mu, and Tn7, the tniA gene was followed by a gene, tniB, for a probable ATP-binding protein. The ends of Tn5090, like those of most other elements producing D,D(35)E proteins, begin by 5'-TG and also contains a complex structure with four 19-bp repeats at the left end and three at the right end. Similarly organized repeats have been observed earlier at the termini of both Tn7 and phage Mu, where they bind their respective transposases and have a role in holoenzyme assembly. Another open reading frame observed in Tn5090, tniC, codes for a recombinase of the invertase/resolvase family, suggesting a replicative transposition mechanism. The data presented here suggest that Tn5090, Tn7, Tn552, and Mu form a subfamily of bacterial transposons which in parallel to many insertion sequences are related to the retroelements. PMID:8195081

  19. Molecular determinants of cardiac myocyte performance as conferred by isoform-specific TnI residues.

    PubMed

    Thompson, Brian R; Houang, Evelyne M; Sham, Yuk Y; Metzger, Joseph M

    2014-05-20

    Troponin I (TnI) is the molecular switch of the sarcomere. Cardiac myocytes express two isoforms of TnI during development. The fetal heart expresses the slow skeletal TnI (ssTnI) isoform and shortly after birth ssTnI is completely and irreversibly replaced by the adult cardiac TnI (cTnI) isoform. These two isoforms have important functional differences; broadly, ssTnI is a positive inotrope, especially under acidic/hypoxic conditions, whereas cTnI facilitates faster relaxation performance. Evolutionary directed changes in cTnI sequence suggest cTnI evolved to favor relaxation performance in the mammalian heart. To investigate the mechanism, we focused on several notable TnI isoform and trans-species-specific residues located in TnI's helix 4 using structure/function and molecular dynamics analyses. Gene transduction of adult cardiac myocytes by cTnIs with specific helix 4 ssTnI substitutions, Q157R/A164H/E166V/H173N (QAEH), and A164H/H173N (AH), were investigated. cTnI QAEH is similar in these four residues to ssTnI and nonmammalian chordate cTnIs, whereas cTnI AH is similar to fish cTnI in these four residues. In comparison to mammalian cTnI, cTnI QAEH and cTnI AH showed increased contractility and slowed relaxation, which functionally mimicked ssTnI expressing myocytes. cTnI QAEH molecular dynamics simulations demonstrated altered intermolecular interactions between TnI helix 4 and cTnC helix A, specifically revealing a new, to our knowledge, electrostatic interaction between R171of cTnI and E15 of cTnC, which structurally phenocopied the ssTnI conformation. Free energy perturbation calculation of cTnC Ca(2+) binding for these conformations showed relative increased calcium binding for cTnI QAEH compared to cTnI. Taken together, to our knowledge, these new findings provide evidence that the evolutionary-directed coordinated acquisition of residues Q157, A164, E166, H173 facilitate enhanced relaxation performance in mammalian adult cardiac myocytes. PMID:24853739

  20. Tn502 and Tn512 Are res Site Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites ?

    PubMed Central

    Petrovski, Steve; Stanisich, Vilma A.

    2010-01-01

    In this study, we report on the transposition behavior of the mercury(II) resistance transposons Tn502 and Tn512, which are members of the Tn5053 family. These transposons exhibit targeted and oriented insertion in the par region of plasmid RP1, since par-encoded components, namely, the ParA resolvase and its cognate res region, are essential for such transposition. Tn502 and, under some circumstances, Tn512 can transpose when par is absent, providing evidence for an alternative, par-independent pathway of transposition. We show that the alternative pathway proceeds by a two-step replicative process involving random target selection and orientation of insertion, leading to the formation of cointegrates as the predominant product of the first stage of transposition. Cointegrates remain unresolved because the transposon-encoded (TniR) recombination system is relatively inefficient, as is the host-encoded (RecA) system. In the presence of the res-ParA recombination system, TniR-mediated (and RecA-mediated) cointegrate resolution is highly efficient, enabling resolution both of cointegrates involving functional transposons (Tn502 and Tn512) and of defective elements (In0 and In2). These findings implicate the target-encoded accessory functions in the second stage of transposition as well as in the first. We also show that the par-independent pathway enables the formation of deletions in the target molecule. PMID:20118251

  1. 75 FR 27009 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ...27009] [FR Doc No: 2010-11482...12161 and 12162] Tennessee Disaster Number TN-00038...Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding, Straight-Line...

  2. 75 FR 38155 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ...38155-38156] [FR Doc No: 2010-16060...12159 and 12160] Tennessee Disaster Number TN-00039...disaster for the State of Tennessee (FEMA-1909-DR), dated 05/04/ 2010. Incident: Severe Storms, Flooding, Straight-line...

  3. 75 FR 29591 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-26

    ...29591] [FR Doc No: 2010-12589] [[Page 29591...12161 and 12162] Tennessee Disaster Number TN-00038...Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding, Straight-Line...

  4. 75 FR 27008 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ...27008] [FR Doc No: 2010-11405...12159 and 12160] Tennessee Disaster Number TN-00039...disaster for the State of TENNESSEE (FEMA-1909-DR), dated 05/04/ 2010. Incident: Severe Storms, Flooding, Straight-line...

  5. 75 FR 27845 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ...27845] [FR Doc No: 2010-11749...12161 and 12162] Tennessee Disaster Number TN-00038...Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding, Straight-Line...

  6. 75 FR 30874 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ...30874] [FR Doc No: 2010-13180...12161 and 12162] Tennessee Disaster Number TN-00038...Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding, Straight-Line...

  7. 75 FR 29590 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-26

    ...29590] [FR Doc No: 2010-12590...12159 and 12160] Tennessee Disaster Number TN-00039...disaster for the State of Tennessee (FEMA-1909-DR, dated 05/04/ 2010. Incident: Severe Storms, Flooding, Straight-line...

  8. 75 FR 27008 - TENNESSEE Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ...27008] [FR Doc No: 2010-11411...12161 and 12162] TENNESSEE Disaster Number TN-00038...Only for the State of TENNESSEE (FEMA-1909-DR), dated 05/04/2010 . Incident: Severe Storms, Flooding, Straight-Line...

  9. 75 FR 35103 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ...35103] [FR Doc No: 2010-14893...12159 and 12160] Tennessee Disaster Number TN-00039...disaster for the State of Tennessee (FEMA-1909-DR), dated 05/04/ 2010. Incident: Severe Storms, Flooding, Straight-line...

  10. 75 FR 35103 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ...35103] [FR Doc No: 2010-14896...12161 and 12162] Tennessee Disaster Number TN-00038...Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding, Straight-Line...

  11. 75 FR 30870 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ...30870-30871] [FR Doc No: 2010-13179...12159 and 12160] Tennessee Disaster Number TN-00039...disaster for the State of Tennessee (FEMA-1909-DR), dated 05/04/ 2010 . Incident: Severe Storms, Flooding, Straight-line...

  12. 75 FR 27009 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ...27009-27010] [FR Doc No: 2010-11407...12159 and 12160] Tennessee Disaster Number TN-00039...disaster for the State of TENNESSEE (FEMA-1909-DR), dated 05/04/ 2010. Incident: Severe Storms, Flooding, Straight-line...

  13. Expression of a Tn-like epitope by carcinoma cells.

    PubMed Central

    Roxby, D. J.; Skinner, J. M.; Morley, A. A.; Weeks, S.; Burpee, M.

    1987-01-01

    A monoclonal antibody, FBT3, was raised against Tn positive erythrocytes and, using immunohistochemistry, fresh and fixed tissues from patients with cancer were studied to detect any expression of a Tn-like epitope. Expression was found in neoplastic cells, usually both in cytoplasm and on cell membranes, from 104 of 147 cases of carcinoma and 1 of 13 cases of lymphoma, but rarely in adjacent, morphologically normal cells. Tn expression was seen in some normal glandular cells but, unlike cancer cells, it was distributed as fine granules in a supranuclear position. Detection of a Tn-like epitope is of theoretical interest and may be of direct diagnostic value. Images Figure 1 Figure 2 Figure 3 PMID:2449235

  14. NCAR/TN-450+IA NCAR TECHNICAL NOTE

    E-print Network

    Potter, Don

    .4 Information transfer in genetic algorithms . .. . . . .. . . . . .. . . . .. . . . 25 3. PIKAIA: A genetici NCAR/TN-450+IA NCAR TECHNICAL NOTE March 2002 AN INTRODUCTION TO GENETIC ALGORITHMS FOR NUMERICAL, optimization, and genetic algorithms 2.1 Biological evolution

  15. Structural and Kinetic Effects of PAK3 phosphorylation mimic of cTnI(S151E) on the cTnC-cTnI Interaction in the Cardiac Thin Filament

    PubMed Central

    Ouyang, Yexin; Mamidi, Ranganath; Jayasundar, Jayant James; Chandra, Murali; Dong, Wen-Ji

    2010-01-01

    Residue Ser151 of cTnI is known to be phosphorylated by p21-activated kinase 3 (PAK3). It has been found that PAK3-mediated phosphorylation of cTnI induces an increase in myofilament Ca2+ sensitivity, but the detailed mechanism is unknown. We investigated how the structural and kinetic effects mediated by pseudo-phosphorylation of cTnI (S151E) modulates Ca2+-induced activation of cardiac thin filaments. Using steady-state, time-resolved Förster Resonance Energy Transfer (FRET) and stopped-flow kinetic measurements, we monitored Ca2+-induced changes in cTnI-cTnC interactions. Measurements were done using reconstituted thin filaments, which contained the pseudo-phosphorylated cTnI(S151E). We hypothesized that the thin filament regulation is modulated by altered cTnC-cTnI interactions due to charge modification caused by the phosphorylation of Ser151 in cTnI. Our results showed that the pseudo-phosphorylation of cTnI (S151E) sensitizes structural changes to Ca2+ by shortening the intersite distances between cTnC and cTnI. Furthermore, kinetic rates of Ca2+ dissociation-induced structural change in the regulatory region of cTnI were significantly reduced by cTnI (S151E). The aforementioned effects of pseudo-phosphorylation of cTnI were similar to the effects of strong crossbridges on structural changes in cTnI. Our results provide novel information on how cardiac thin filament regulation is modulated by PAK3 phosphorylation of cTnI. PMID:20540949

  16. TnT -- A Statistical Part-of-Speech Tagger

    Microsoft Academic Search

    Thorsten Brants

    2000-01-01

    Trigrams'n'Tags (TnT) is an efficient statistical part-of-speech tagger. Contrary to claims found elsewhere in the literature, we argue that a tagger based on Markov models performs at least as well as other current approaches, including the Maximum Entropy framework. A recent comparison has even shown that TnT performs significantly better for the tested corpora. We describe the basic model of

  17. Characterization of transposon Tn5469 from the cyanobacterium Fremyella diplosiphon.

    PubMed Central

    Kahn, K; Schaefer, M R

    1995-01-01

    A transposon, designated Tn5469, was isolated from mutant strain FdR1 of the filamentous cyanobacterium Fremyella diplosiphon following its insertion into the rcaC gene. Tn5469 is a 4,904-bp noncomposite transposon with 25-bp near-perfect terminal inverted repeats and has three tandemly arranged, slightly overlapping potential open reading frames (ORFs) encoding proteins of 104.6 kDa (909 residues), 42.5 kDa (375 residues), and 31.9 kDa (272 residues). Insertion of Tn5469 into the rcaC gene in strain FdR1 generated a duplicate 5-bp target sequence. On the basis of amino acid sequence identifies, the largest ORF, designated tnpA, is predicted to encode a composite transposase protein. A 230-residue domain near the amino terminus of the TnpA protein has 15.4% amino acid sequence identity with a corresponding domain for the putative transposase encoded by Lactococcus lactis insertion sequence S1 (ISS1). In addition, the sequence for the carboxyl-terminal 600 residues of the TnpA protein is 20.0% identical to that for the TniA transposase encoded by Tn5090 on Klebsiella aerogenes plasmid R751. The TnpA and TniA proteins contain the D,D(35)E motif characteristic of a recently defined superfamily consisting of bacterial transposases and integrase proteins of eukaryotic retroelements and retrotransposons. The two remaining ORFs on Tn5469 encode proteins of unknown function. Southern blot analysis showed that wild-type F. diplosiphon harbors five genomic copies of Tn5469. In comparison, mutant strain FdR1 harbors an extra genomic copy of Tn5469 which was localized to the inactivated rcaC gene. Among five morphologically distinct cyanobacterial strains examined, none was found to contain genomic sequences homologous to Tn5469. PMID:8522506

  18. Chromosomal complementation using Tn7 transposon vectors in Enterobacteriaceae.

    PubMed

    Crépin, Sébastien; Harel, Josée; Dozois, Charles M

    2012-09-01

    Genetic complementation in many bacteria is commonly achieved by reintroducing functional copies of the mutated or deleted genes on a recombinant plasmid. Chromosomal integration systems using the Tn7 transposon have the advantage of providing a stable single-copy integration that does not require selective pressure. Previous Tn7 systems have been developed, although none have been shown to work effectively in a variety of enterobacteria. We have developed several mini-Tn7 and transposase vectors to provide a more versatile system. Transposition of Tn7 at the chromosomal attTn7 site was achieved by a classical conjugation approach, wherein the donor strain harbored the mini-Tn7 vector and the recipient strain possessed the transposase vector. This approach was efficient for five different pathogenic enterobacterial species. Thus, this system provides a useful tool for single-copy complementation at an episomal site for research in bacterial genetics and microbial pathogenesis. Furthermore, these vectors could also be used for the introduction of foreign genes for use in biotechnology applications, vaccine development, or gene expression and gene fusion constructs. PMID:22706059

  19. 78 FR 52424 - Amendment of Class E Airspace; Dayton, TN, Establishment of Class E Airspace; Cleveland, TN, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-23

    ...establishes Class E Airspace at Cleveland Regional Jetport, Cleveland, TN, to accommodate area navigation (RNAV) global positioning system (GPS) SIAPs at the airport. Information regarding Bradley Memorial Hospital is added to the Cleveland,...

  20. Transposon mutagenesis in halophilic eubacteria: conjugal transfer and insertion of transposon Tn5 and Tn1732 in Halomonas elongata.

    PubMed

    Kunte, H J; Galinski, E A

    1995-05-15

    Molecular genetic studies of halophilic eubacteria have been limited by the lack of a suitable method for mutagenesis. To overcome this, we established a transposon mutagenesis procedure for the ectoine-producing, halophilic bacterium Halomonas elongata. We used suicide plasmids pSUP101 and pSUP102-Gm to introduce the transposons Tn5 and Tn1732 respectively into H. elongata via Escherichia coli SM10 mediated conjugation. Our finding that H. elongata is sensitive to aminoglycoside antibiotics at low salinity enabled us to apply transposons that mediate kanamycin resistance. The insertions of transposon Tn1732 occurred at different sites in the chromosome of H. elongata, as proved by Southern hybridization analysis. Phenotypic analysis revealed that different auxotrophic and salt sensitive mutants were generated by mutagenesis with transposon Tn1732. To our knowledge this is the first report of a successful application of a transposon for direct generalized mutagenesis in a halophilic eubacterium. PMID:7781978

  1. Vertical transmission of TnSNPV, TnCPV, AcMNPV, and possibly recombinant NPV in Trichoplusia ni

    E-print Network

    Hammock, Bruce D.

    (TnCPV), Autographa californica nucleopolyhedrovirus (AcMNPV), and AcMNPV engineered to express). All rights reserved. Keywords: Trichoplusia ni; Nucleopolyhedrovirus; Trichoplusia ni NPV; Autographa californica NPV; Recombinant NPV; Cypovirus; Trichoplusia ni CPV; Transmission, viral; Transmission, vertical

  2. The Cosmc connection to the Tn antigen in cancer.

    PubMed

    Ju, Tongzhong; Aryal, Rajindra P; Kudelka, Matthew R; Wang, Yingchun; Cummings, Richard D

    2014-01-01

    The Tn antigen is a tumor-associated carbohydrate antigen that is not normally expressed in peripheral tissues or blood cells. Expression of this antigen, which is found in a majority of human carcinomas of all types, arises from a blockage in the normal O-glycosylation pathway in which glycans are extended from the common precursor GalNAc?1-O-Ser/Thr (Tn antigen). This precursor is generated in the Golgi apparatus on newly synthesized glycoproteins by a family of polypeptide ?-N-acetylgalactosaminyltransferases (ppGalNAcTs) and then extended to the common core 1 O-glycan Gal?1-3GalNAc?1-O-Ser/Thr (T antigen) by a single enzyme termed the T-synthase (core 1 ?3-galactosyltransferase or C1GalT). Formation of the active form of the T-synthase requires a unique molecular chaperone termed Cosmc, encoded by Cosmc on the X-chromosome (Xq24 in humans, Xc3 in mice). Cosmc resides in the endoplasmic reticulum (ER) and prevents misfolding, aggregation, and proteasome-dependent degradation of newly synthesized T-synthase. Loss of expression of active T-synthase or Cosmc can lead to expression of the Tn antigen, along with its sialylated version Sialyl Tn antigen as observed in several cancers. Both genetic and epigenetic pathways, in addition to potential metabolic regulation, can result in abnormal expression of the Tn antigen. Engineered expression of the Tn antigen by disruption of either C1GalT (T-syn) or Cosmc in mice is associated with a tremendous range of pathologies and engineered expression of the Tn antigen in mouse embryos leads to embryonic death. Studies indicate that many membrane glycoproteins expressing the Tn antigen and/or truncated O-glycans may be dysfunctional, due to degradation and/or misfolding. Thus, expression of normal O-glycans is associated with health and homeostasis whereas truncation of O-glycans, e.g. the Tn and/or Sialyl Tn antigens is associated with cancer and other pathologies. PMID:24643043

  3. 76 FR 32387 - Tennessee Disaster Number TN-00053

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-06

    ...Primary Counties: (Physical Damage and Economic Injury Loans): Gibson, Lauderdale. Contiguous Counties: (Economic Injury Loans...Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster...

  4. The Lower Sevier River Basin Crop Monitor and Forecast Decision Support System: Exploiting Landsat Imagery to Provide Continuous Information to Farmers and Water Managers

    NASA Astrophysics Data System (ADS)

    Torres-Rua, A. F.; Walker, W. R.; McKee, M.

    2013-12-01

    The last century has seen a large number of innovations in agriculture such as better policies for water control and management, upgraded water conveyance, irrigation, distribution, and monitoring systems, and better weather forecasting products. In spite of this, irrigation management and irrigation water deliveries by farmers/water managers is still based on factors like water share amounts, tradition, and past experience on irrigation. These factors are not necessarily related to the actual crop water use; they are followed because of the absence of related information provided in a timely manner at an affordable cost. Thus, it is necessary to develop means to deliver continuous and personalized information about crop water requirements to water users/managers at the field and irrigation system levels so managers at these levels can better quantify the required versus available water for irrigation during the irrigation season. This study presents a new decision support system (DSS) platform that addresses the absence of information on actual crop water requirements and crop performance by providing continuous updated farm-based crop water use along with other farm performance indicators such as crop yield and farm management to irrigators and water managers. This DSS exploits the periodicity of the Landsat Satellite Mission (8 to 16 days, depending on the period of interest) to provide remote monitoring at the individual field and irrigation system levels. The Landsat satellite images are converted into information about crop water use, yield performance and field management through application of state-of-the-art semi-physical and statistical algorithms that provide this information at a pixel basis that are ultimately aggregated to field and irrigation system levels. A version of the DSS has been implemented for the agricultural lands in the Lower Sevier River, Utah, and has been operational since the beginning of the 2013 irrigation season. The main goal of this DSS implementation is to provide continuous and personalized information to farmers and water managers regarding crops in fields and the irrigation delivery system throughout the irrigation season so that decisions related to agricultural water use can result in water savings while not diminishing crop yields.

  5. Low-temperature thermochronology of the Laramide Ranges and eastward translation of shortening in the Sevier Belt, Wyoming, Utah and Montana

    NASA Astrophysics Data System (ADS)

    Peyton, Sara Lynn

    This dissertation contains two studies that use very different techniques to investigate the Mesozoic and Cenozoic tectonics of the western USA. The first study investigates shortening in the Sevier thrust belt of northeast Utah and southwest Wyoming using cross sections and seismic reflection data. The second study investigates the low-temperature thermochronology of the Laramide Ranges using apatite (U-Th)/He dating. We used cross sections and seismic reflection data to investigate bed length discrepancies within the hanging wall of the Absaroka thrust in the Sevier thrust belt of northeast Utah and southwest Wyoming. Restoration of cross sections suggests that there was ˜8-14 km of pre-Absaroka-thrust shortening above the Jurassic Preuss salt detachment, but not below it, in the hanging wall of the Absaroka thrust. Reflection seismic data over the hanging wall of the Crawford thrust show that the Crawford thrust is not offset along the Preuss salt detachment, indicating that the additional shortening on the Absaroka plate was transferred east before main movement on the Crawford thrust. Although early displacement on the Crawford thrust cannot be ruled out as the cause of the extra shortening, surface and subsurface geology suggests slip from the western thrust system (Willard and Lost Creek thrusts) was transferred several tens of kilometers east along the Jurassic Preuss salt detachment between ˜102-90 Ma, to the future location of the Absaroka thrust hanging wall. The lack of deformation of the Crawford thrust on the seismic data, along with shortening and extension estimates from cross sections, also indicate that the magnitude of Paleocene and post-early Eocene shortening on the Medicine Butte thrust was essentially offset by subsequent extension on the middle Eocene to late Oligocene Almy-Acocks normal-fault system. For the second study in this dissertation, we dated 91 borehole and surface samples from Laramide-age, basement-cored uplifts of the Rocky Mountain foreland (Wind River, Beartooth, Bighorn and Laramie Ranges) and the Uncompahgre Uplift using the apatite (U-Th)/He system. Apatite (U-Th)/He ages generally decrease with increasing subsurface depth (decreasing elevation) and most samples show age dispersion ranging from tens to hundreds of Myr. Additionally, several samples show correlations between apatite (U-Th)/He age and effective U concentration (eU = [U] + 0.235[Th]) of the crystal, indicating that radiation damage has affected He diffusivity, and hence (U-Th)/ He age. Many surface and near-surface samples have apatite (U-Th)/He ages that are older than corresponding apatite fission-track ages. Forward modeling of Laramide-type thermal histories using a radiation damage diffusion model showed that (U-Th)/He ages may be widely dispersed, and may be older than corresponding apatite fission-track ages within a fossil He partial retention zone. Most of our samples, however, do not show the correlation between (U-Th)/He age and eU predicted by radiation damage diffusion models. We investigated the influence of both grain size and eU content and show that the effects of grain size can obscure (U-Th)/ He age-eU correlations and, similarly, the effect of eU variation can obscure (U-Th)/ He age-grain size correlations. (U-Th)/He ages that are older than fission-track ages from high peaks in the Wind River Range, and from some samples from the Beartooth Range, are most likely the result of He implantation from high eU phases. Best-fit thermal histories from the inversion of age-eU pairs were extrapolated to other elevations to create model age-elevation profiles for a range of eU concentrations. These model profiles approximate our real data. Inverse modeling of (U-Th)/He age data suggests that rapid exhumation within the Laramide province likely began earlier in the Bighorn Mountains (before ˜71 Ma) than the Beartooth Range (before ˜58 Ma), and that the borehole at the northern end of the Laramie Range penetrated a fault sliver at depth.

  6. Functional characterization of mouse fetal TnI gene promoters in myocardial cells

    Microsoft Academic Search

    J. Du; C. Nan; J. J. Huang; C. Zhang; J. Liu; P. Jia; M. Abers; X. P. Huang

    2008-01-01

    Two major troponin I (TnI) genes, fetal TnI (ssTnI) and adult TnI (cTnI), are expressed in the mammalian heart under the control\\u000a of a developmentally regulated program. In this study, the up-stream domain (?1,800 bp) of mouse fetal TnI gene has been cloned\\u000a and characterized. There is a high homology of this region among mouse, rat and human. Analysis of the

  7. 75 FR 32201 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ...quality of forest habitat to provide for...and protect bald eagle nesting sites and count...ample foraging habitat. Alternative D...quality of forest habitat to provide for...and protect bald eagle nesting sites and...

  8. 76 FR 5194 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN; Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ...Wildlife observation and photography, (2) environmental education...cooperative farming, (6) scientific research, (7) commercial...wildlife observation, wildlife photography, and environmental education...increase wildlife observation/photography opportunities with the...

  9. 75 FR 6257 - Watts Bar Reservoir Land Management Plan, Loudon, Meigs, Rhea, and Roane Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ...TNSHPO). The U.S. Forest Service acknowledged...be monitored for water quality effects. EPA recommended...on watershed and water quality issues. TVA continues to monitor water quality in its reservoirs and...erosion-control work would utilize seed mixes comprised of...

  10. ERDC TN-DOER-R18 October 2011

    E-print Network

    US Army Corps of Engineers

    ERDC TN-DOER-R18 October 2011 An Introduction to Us ing B ayes ian Networks to Model Dredging Decis introduction to Bayesian networks and discusses how they might be applied to model dredging decisions a detailed example demonstrating how Bayesian networks could be used to model navigation dredging decisions

  11. Fogelman Executive Center and Conference Hotel Memphis, TN

    E-print Network

    Cui, Yan

    will be held at the Fogelman Executive Conference Center, 330 Innovation Drive, Memphis, TN 38152. PARKING Conference Center at 330 Innovation Drive. Please bring in your white ticket to exchange for an exit ticket stages to prevent secondary complications. · Recognize new areas of research and treatment

  12. EMPLOYMENT SUMMARY FOR 2011 GRADUATES Memphis, TN 38103

    E-print Network

    Dasgupta, Dipankar

    EMPLOYMENT SUMMARY FOR 2011 GRADUATES Memphis, TN 38103 Website : www.memphis.edu/law/ Phone : 901 graduates 129 Unemployed - Not Seeking 2 Employment Status Unknown 22 Unemployed - Seeking 9 Employed - Undeterminable * 0 0 0 0 0 Employed - Bar Passage Required 77 2 2 0 81 Pursuing Graduate Degree Full Time 1

  13. National Association of Counties

    NSDL National Science Digital Library

    2002-01-01

    With 3066 U.S. counties, the National Association of Counties (NACo) has the arduous task of making sure the nations counties are acknowledged and understood in the halls of the White House and Congress. NACo, the only national government organization devoted to highlighting and improving the understanding of county issues, "collects, researches, publishes and disseminates a variety of different information for, on and about counties." On their Web site, viewers have access to a comprehensive and in-depth database of county information including county population; census bureau quick facts; elected county officials; county codes and ordinances; county policies; links to Capitolimpact.com, which provides nationwide county statistics such as economic and demographic data; and much more. This site is easily navigable, has counties arranged alphabetically by state, and would be of value to anyone living inside a county domain --- which is just about everyone.

  14. 8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY VIEW OF ABUTMENT SUPPORT INTO THE GROUND - Lost Creek Road Bridge, Spanning Sevier River at County Road 243, 2 miles east of Aurora, Aurora, Sevier County, UT

  15. 78 FR 27029 - Modification of Class C Airspace; Nashville International Airport; TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ...No. 12-AWA-7] Modification of Class C Airspace; Nashville International Airport...Nashville International Airport, TN, Class C airspace area by removing a cutout from the...Nashville International Airport, TN, Class C airspace area (78 FR 6257)....

  16. Dissecting Tn5 transposition using HIV-1 integrase diketoacid inhibitors.

    PubMed

    Czyz, Agata; Stillmock, Kara A; Hazuda, Daria J; Reznikoff, William S

    2007-09-25

    Diketoacid (DKA) compounds have been shown to inhibit HIV-1 integrase by a mechanism that involves sequestration of the active site metals. Because HIV-1 integrase and Tn5 transposase have similar active site architectures and catalytic mechanisms, we investigated whether DKA analogues would inhibit Tn5 transposase activity and provide a model system to explore the mechanisms of action of these inhibitors. A screen of several hundred DKA analogues identified several with activity against Tn5 Tnp. Six DKA inhibitors used in this study manifested a variety of effects on different transposition steps suggesting that different analogues may have different binding contacts with transposase. All DKA compounds inhibited paired end complex (PEC) formation in which the nucleoprotein complex required for catalysis is assembled. Dissociation of PECs by some DKA compounds indicates that these inhibitors can decrease PEC stability. Four DKA compounds inhibited the two cleavage steps releasing transposon DNA from flanking DNA, and one of these four compounds preferentially inhibited the second cleavage step. The differential effect of this inhibitor on the second cleavage event indicates that cleavage of the two transposon-donor DNA boundaries is a sequential process requiring a conformational change. The requirement for a conformational change between cleavage events was also demonstrated by the inability of transposase to perform second cleavage at 25 degrees C. Finally, all six compounds inhibit strand transfer, the final step of Tn5 transposition. Two of the compounds that inhibited strand transfer have no effect on DNA cleavage. The strand transfer inhibition properties of various DKA compounds was sensitive to the structure of the 5'-non-transferred strand, suggesting that these compounds bind in or near the transposase active site. Other results that probe compound binding sites include the effects of active site mutations and donor DNA on DKA compound inhibition activities. Thus, DKA inhibitors will provide an important set of tools to investigate the mechanism of action of transposases and integrases. PMID:17725323

  17. Tn5-Induced Mutations in the Enterobacterial Phytopathogen Erwinia chrysanthemi†

    PubMed Central

    Chatterjee, Arun K.; Thurn, Kerry K.; Feese, Dorothy A.

    1983-01-01

    Escherichia coli (2492/pJB4JI) matings with Erwinia chrysanthemi produced kanamycin resistant (Kmr) transconjugants, a majority of which were gentamicin sensitive (Gms). A small proportion (about 0.8%) of the Kmr Gms clones were either auxotrophic or failed to catabolize galacturonate (Gtu?). The R plasmid (pJB4JI) DNA was detected in the parent E. coli strain and in a Kmr Gmr transconjugant, but not in Kmr GmsE. chrysanthemi strains carrying Tn5-induced mutations. In Hfr crosses, Kmr (Tn5) was found linked with most mutations. A majority (>95%) of prototrophic recombinants were Kms, except for Leu+ and Arg+ recombinants which were 30 to 50% Kms. Spontaneous revertants were obtained for all markers except car, gtu, lys, thr, and trp. Prototrophic revertants, with the exception of Met+, Leu+, or His+ clones, were Kms. We conclude from both genetic and physical data that Tn5 transposed from pJB4JI into different sites on the chromosome of E. chrysanthemi. Images PMID:16346212

  18. Characterization of Tn6238 with a New Allele of aac(6')-Ib-cr.

    PubMed

    Quiroga, María P; Orman, Betina; Errecalde, Laura; Kaufman, Sara; Centrón, Daniela

    2015-05-01

    Here, we report that the genetic structure of Tn1331 remained conserved in Argentina from 1989 to 2013 (72 of 73 isolates), with the exception being the plasmid-borne Tn1331-like transposon Tn6238 containing a new aac(6')-Ib-cr allele recovered from a colistin-resistant Klebsiella pneumoniae clinical isolate. A bioinformatic analysis of aac(6')-Ib-like gene cassettes suggests that this new aac(6')-Ib-cr allele emerged through mutation or homologous recombination in the Tn1331 genetic platform. Tn6238 is a novel platform for the dissemination of aminoglycoside and fluoroquinolone resistance determinants. PMID:25691640

  19. Tumor-associated Neu5Ac-Tn and Neu5Gc-Tn antigens bind to C-type lectin CLEC10A (CD301, MGL).

    PubMed

    Mortezai, Naghmeh; Behnken, Henning N; Kurze, Anna-Katharina; Ludewig, Peter; Buck, Friedrich; Meyer, Bernd; Wagener, Christoph

    2013-07-01

    In human tumors, glycoproteins often exhibit abnormal glycosylation patterns, e.g. certain Lewis structures, TF antigen, Tn antigen and/or their sialylated forms, creating additional binding sites for glycoreceptors. In the present study, we have analyzed the carbohydrate specificity of the C-type lectin CLEC10A using glycan profiling by enzyme-linked immunosorbent assay (ELISA). In addition to the known ligands, we show binding to two tumor-associated antigens, namely Neu5Ac?2,6-Tn and Neu5Gc?2,6-Tn, with an affinity of CLEC10A in the micromolar range. Detailed analyses of the glycan-lectin interactions were carried out by surface plasmon resonance (SPR) and saturation transfer difference (STD) NMR. CLEC10A binds Neu5Ac?2,6-Tn and Neu5Gc?2,6-Tn with dissociation constants of 297 and 80 µM, respectively, as determined by SPR. Comparison of the STD nuclear magnetic resonance (NMR) binding epitopes of Tn and Neu5Ac?2,6-Tn revealed a constant binding mode of the N-acetylgalactosamine moiety. This finding is in good agreement with binding studies of CLEC10A transfectomas, which show a well-defined interaction of transmembrane CLEC10A with 6-sialylated-Tn structures. Since both Neu5Ac?2,6-Tn and Neu5Gc?2,6-Tn together with the previously known Tn antigen are expressed in human tumors such as mammary carcinoma, the interaction with CLEC10A expressed by macrophages and dendritic cells could be of major functional significance in tumor progression. PMID:23507963

  20. Appendix A - County Codes

    Cancer.gov

    January 1998 SEER Program Code Manual, 3 rd Edition A-1 APPENDIX A COUNTY CODES APPENDIX A COUNTY CODES A-2 SEER Program Code Manual, 3rd Edition January 1998 The following are the valid county codes for coding county of residence at diagnosis: Reference:

  1. Topological strings and 5d T_N partition functions

    E-print Network

    Hirotaka Hayashi; Hee-Cheol Kim; Takahiro Nishinaka

    2014-05-06

    We evaluate the Nekrasov partition function of 5d gauge theories engineered by webs of 5-branes, using the refined topological vertex on the dual Calabi-Yau threefolds. The theories include certain non-Lagrangian theories such as the T_N theory. The refined topological vertex computation generically contains contributions from decoupled M2-branes which are not charged under the 5d gauge symmetry engineered. We argue that, after eliminating them, the refined topological string partition function agrees with the 5d Nekrasov partition function. We explicitly check this for the T_3 theory as well as Sp(1) gauge theories with N_f = 2, 3, 4 flavors. In particular, our method leads to a new expression of the Sp(1) Nekrasov partition functions without any contour integrals. We also develop prescriptions to calculate the partition functions of theories obtained by Higgsing the T_N theory. We compute the partition function of the E_7 theory via this prescription, and find the E_7 global symmetry enhancement. We finally discuss a potential application of the refined topological vertex to non-toric web diagrams.

  2. Nucleotide Sequence Analysis of Integrative Conjugative Element Tn5253 of Streptococcus pneumoniae

    PubMed Central

    Iannelli, Francesco; Santoro, Francesco; Oggioni, Marco R.

    2014-01-01

    Conjugative transposon Tn5253, an integrative conjugative element (ICE) of Streptococcus pneumoniae carrying the cat and tet(M) genes, was shown to be 64,528 bp in size and to contain 79 open reading frames, of which only 38 could be annotated. Two distinct genetic elements were found integrated into Tn5253: Tn5251 (18,033 bp), of the Tn916-Tn1545 family of ICEs, and ?cat(pC194) (7,627 bp), which could not conjugate but was capable of intracellular mobility by excision, circularization, and integration by homologous recombination. The highest conjugation frequency of Tn5253 was observed when Streptococcus pyogenes was the donor (6.7 × 10?3 transconjugants/donor). PMID:24295984

  3. Transfer of Tn5385, a Composite, Multiresistance Chromosomal Element from Enterococcus faecalis

    Microsoft Academic Search

    LOUIS B. RICE; LENORE L. CARIAS

    1998-01-01

    Tn5385 is a ca. 65-kb element integrated into the chromosomes of clinical Enterococcus faecalis strains CH19 and CH116. It confers resistance to erythromycin, gentamicin, mercuric chloride, streptomycin, tetracycline- minocycline, and penicillin via b-lactamase production. Tn5385 is a composite structure containing regions previously found in staphylococcal and enterococcal plasmids. Several transposons and transposon-like ele- ments within Tn5385 have been identified, including

  4. Physical and functional mapping of Tn 2603 , a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance

    Microsoft Academic Search

    Tomoko Yamamoto; Michiyasu Tanaka; Rieko Baba; Saburo Yamagishi

    1981-01-01

    A map of cleavage sites for restriction endonucleases EcoR1, BamHI, HindIII, and SalI on Tn2603, a transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury, was constructed by an analysis of restriction cleavage patterns of plasmid pMK1.:: Tn2603 and its deletion derivative. By cloning the fragments generated from pMK1::Tn2603 with these restriction endonucleases to a pACYC184 plasmid vehicle, the regions

  5. BWR - Spent Fuel Transport and Storage with the TN{sup TM}9/4 and TN{sup TM}24BH Casks

    SciTech Connect

    Wattez, L. [COGEMA LOGISTICS - AREVA Group (France); Marguerat, Y. [BKW FMB Energy Ltd (Switzerland); Hoesli, C. [ZWILAG Zwischenlager Wuerenlingen AG (Switzerland)

    2006-07-01

    The Swiss Nuclear Utilities have started in 2001 to store spent fuel in dry metallic dual-purpose casks at ZWILAG, the Swiss interim storage facility. BKW FMB Energy Ltd., the Muehleberg Nuclear Power Plant owner, is involved in this process and has elected to store its BWR spent fuel in a new high capacity dual-purpose cask, the TNeTeM24BH from the COGEMA Logistics/TRANSNUCLEAR TN{sup TM}24 family. The Muehleberg BWR spent fuels are transported by road in a medium size shuttle transport cask and then transferred to a heavy transport/storage cask (dry transfer) in the hot cell of ZWILAG site. For that purpose, COGEMA Logistics designed and supplied: - Two shuttle casks, TN{sup TM}9/4, mainly devoted to transport of spent fuel from Muehleberg NPP to ZWILAG. Licensed according to IAEA 1996, the TN{sup TM}9/4 is a 40 ton transport cask, for 7 BWR high bum-up spent fuel assemblies. - A series of new high capacity dual-purpose casks, TN{sup TM}24BH, holding 69 BWR spent fuels. Two transport campaigns took place in 2003 and 2004. For each campaign, ten TN{sup TM}9/4 round trips are performed, and one TN{sup TM}24BH is loaded. 5 additional TN{sup TM}24BH are being manufactured for BKW, and the next transport campaigns are scheduled from 2006. The TN{sup TM}24BH high capacity dual purpose cask and the TN{sup TM}9/4 transport cask characteristics and capabilities will then be detailed. (authors)

  6. Nonlinear pressure dependence of TN in almost multiferroic EuTiO3.

    PubMed

    Guguchia, Z; Caslin, K; Kremer, R K; Keller, H; Shengelaya, A; Maisuradze, A; Bettis, J L; Köhler, J; Bussmann-Holder, A; Whangbo, M-H

    2013-09-18

    The antiferromagnetic (AFM) phase transition temperature TN of EuTiO3 has been studied as a function of pressure p. The data reveal a nonlinear dependence of TN on p with TN increasing with increasing pressure. The exchange interactions exhibit an analogous dependence on p as TN (if the absolute value of the nearest neighbor interaction is considered) and there is evidence that the AFM transition is robust with increasing pressure. The corresponding Weiss temperature ?W remains anomalous since it always exhibits positive values. The data are analyzed within the Bloch power law model and provide excellent agreement with experiment. PMID:23963024

  7. Nucleotide sequences specific for Tn1545-like conjugative transposons in pneumococci and staphylococci resistant to tetracycline.

    PubMed Central

    Poyart-Salmeron, C; Trieu-Cuot, P; Carlier, C; Courvalin, P

    1991-01-01

    The distributions of tet(M) and conjugative transposons related to Tn1545 were studied by hybridization in 47 clinical isolates of Streptococcus pneumoniae resistant to tetracycline. Resistance to tetracycline was always associated with resistance to minocycline and was due to the presence of the tet(M) gene. Association of tet(M) with int-Tn, the gene encoding the protein required for the movements of Tn1545-like transposons, was found in all but one strain of S. pneumoniae. In contrast, int-Tn was detected in only 2 of 37 strains of Staphylococcus spp. harboring tet(M). Images PMID:1656870

  8. A restriction enzyme cleavage map of Tn 5 and location of a region encoding neomycin resistance

    Microsoft Academic Search

    Richard A. Jorgensen; Steven J. Rothstein; Williams S. Reznikoff

    1979-01-01

    This paper reports a cleavage site map of Tn5 for restriction enzymes BamHI, BglI, BglII, HindII, HindIII, HpaI, SalI, AvaI, SmaI, XhoI, PstI, PvuII, HaeII and HaeIII that was determined by the analysis of restriction enzyme cleavage patterns of ColEl, two independent ColE1::Tn5 plasmids, and a ColE1::Tn5 deletion derivative. BalI, EcoRI, KpnI, and PvuI do not cleave Tn5. Construction and

  9. Geological and petrophysical characterization of the Ferron Sandstone for 3-D simulation of a fluvial-deltaic reservoir. Deliverable 1.4.4: Ferron Sandstone lithofacies and case-study areas, Emery and Sevier Counties, Utah

    SciTech Connect

    Allison, M.L.

    1996-01-04

    The types of dominantly sandstone lithofacies that characterize the Cretaceous Ferron Sandstone reservoir types were tentatively identified before the project began. These reservoir types were defined and mapped at the regional scale and are the subject of the detailed, highly focused case studies. The purpose of conducting detailed case-study analysis is to develop a comprehensive, interdisciplinary, and quantitative characterization of fluvial-deltaic reservoirs which will allow realistic inter-well and reservoir-scale modeling to be used for improved oil-field development in actual reservoirs world-wide. The resulting benefits and value may: (1) increase recoverable reserves by identifying untapped compartments created by reservoir heterogeneity, (2) reduce development costs by more efficiently siting infill drilling locations, (3) increase deliverability by exploiting the reservoir along optimal fluid-flow paths, (4) enhance the application of new technologies, such as horizontal drilling, by identifying optimal drilling directions to maximize fluid-flow, and (5) identify reservoir trends for field extension drilling. Various geologic studies of the Ferron Sandstone were reviewed to compile a list of locations and types of lithofacies in the Ferron Sandstone to be examined in greater detail as part of the subsequent case studies. Preliminary regional interpretations were also used to help select the type and location of lithofacies for case studies. Potential case-study sites were delineated during several reconnaissance field trips by the geologic team. Two case-study sites were selected for the project: Ivie Creek and Willow Springs Wash, in the central and southern parts respectively of the project study area. Results are discussed.

  10. Balance : Lancaster County's tragedy

    E-print Network

    Gingrich, Valerie (Valerie J.)

    2007-01-01

    Lancaster County, Pennsylvania residents are proud of their agricultural heritage. They do not want to see their farmland disappear. But the County continues to be developed into residential subdivisions. This thesis ...

  11. Percent Uninsured by County

    NSDL National Science Digital Library

    ManyEyes

    This is a county by county visualization of the percentage of residents that are uninsured. The data are from a set available here: http://www-958.ibm.com/software/data/cognos/manyeyes/datasets/percent-uninsured-by-county/versions/1

  12. Genetic Diversity of Tn916-Related Transposons among Drug-Resistant Streptococcus pneumoniae Isolates Colonizing Healthy Children in Venezuela?

    PubMed Central

    Quintero, Beatríz; Araque, María; van der Gaast-de Jongh, Christa; Hermans, Peter W. M.

    2011-01-01

    Among a collection of 48 multidrug-resistant pneumococcal strains colonizing healthy children in a small municipality of Mérida, Venezuela, we identified sequence types (STs) related to a variety of internationally spreading drug-resistant clones, as well as ST135, thus far isolated only in Europe. The clones invariably harbored one or more of the Tn916-related transposons Tn3872, Tn5253, Tn6002, Tn2009, and Tn2010. Finally, our data suggest both structural rearrangements in certain transposons and occurrence of novel transposable elements. PMID:21788464

  13. 78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    ...the Tri-Cities, TN area, by correcting the regulatory text of the Class E surface airspace at Tri-Cities...Heliport in the Class E surface area airspace description for...Airspace Designated as Surface Areas * * * * * ASO TN E2...

  14. Immunization of breast cancer patients using a synthetic sialyl-Tn glycoconjugate plus Detox adjuvant

    Microsoft Academic Search

    Grant D. MacLean; Mark Reddish; R. Rao Koganty; Ting Wong; Sham Gandhi; Mark Smolenski; John Samuel; Jean Marc Nabholtzl; B. Michael Longenecker

    1993-01-01

    We have synthesized various formulations that have potential for active specific immunotherapy (ASI) of human cancers. Sialyl-Tn (STn) is a potentially important target structure for ASI because its expression on mucins is a strong, independent predictor of poor prognosis, suggesting that it may have functional significance in the metastatic process. In this first pilot study of synthetic sialyl-Tn hapten conjugated

  15. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ...South Fork National Recreation Area, TN/KY AGENCY: National Park...South Fork National Recreation Area, TN/KY...SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby...South Fork National Recreation Area, Tennessee and Kentucky,...

  16. TN (TRADE NAFTA) STATUS Who and what types of position will qualify?

    E-print Network

    Scharer, John E.

    is on the NAFTA Schedule 2. If the specific job title does not appear on the Schedule, it will be necessary1 TN (TRADE NAFTA) STATUS Who and what types of position will qualify? Citizens of Canada-employment is not permitted. What are the benefits of TN status? The primary benefit is the speed and ease of the application

  17. 1 TN Dept Questionnaire.docx; Feb 2012 INTERNATIONAL SERVICES OFFICE UNIVERSITY OF ROCHESTER

    E-print Network

    Mahon, Bradford Z.

    performed by the foreign national. TN work authorization is granted by USCIS or the Trade officer NOTE: The TN nonimmigrant status is specific to the foreign national, the employer, and the job being at the port of entry to a specific foreign national, only authorizes the foreign national to work

  18. Random insertion of transposon Tn4001 in the genome of Spiroplasma citri strain GII3.

    PubMed

    Foissac, X; Saillard, C; Bové, J M

    1997-01-01

    Electroporation of Spiroplasma citri strain GII3 with plasmid pMUT containing the Staphylococcus aureus transposon Tn4001 resulted in random insertion of Tn4001 into the spiroplasmal genome. Transformation frequencies reached 10(-8) per colony-forming unit (CFU) when 100 microg of plasmid DNA and 3 x 10(9) S. citri CFU were used. Three other strains of S. citri failed to be transformed under the same conditions. In most cases Tn4001 was randomly inserted in the genome of S. citri strain GII3, without insertion of the carrier plasmid. For most transformed spiroplasmas, Tn4001 was stably maintained in the absence of antibiotic selection for at least 80 bacterial generations, making Tn4001 a potential tool for S. citri mutagenesis. PMID:9073584

  19. Identification of basepairs within Tn5 termini that are critical sfor H-NS binding to the transpososome and regulation of Tn5 transposition

    PubMed Central

    2012-01-01

    Background The H-NS protein is a global regulator of gene expression in bacteria and can also bind transposition complexes (transpososomes). In Tn5 transposition H-NS promotes transpososome assembly in vitro and disruption of the hns gene causes a modest decrease in Tn5 transposition (three- to five-fold). This is consistent with H-NS acting as a positive regulator of Tn5 transposition. Molecular determinants for H-NS binding to the Tn5 transpososome have not been determined, nor has the strength of the interaction been established. There is also uncertainty as to whether H-NS regulates Tn5 transposition in vivo through an interaction with the transposition machinery as disruption of the hns gene has pleiotropic effects on Escherichia coli, the organism used in this study. Results In the current work we have further examined determinants for H-NS binding to the Tn5 transpososome through both mutational studies on Tn5 termini (or 'transposon ends') and protein-protein cross-linking analysis. We identify mutations in two different segments of the transposon ends that abrogate H-NS binding and characterize the affinity of H-NS for wild type transposon ends in the context of the transpososome. We also show that H-NS forms cross-links with the Tn5 transposase protein specifically in the transpososome, an observation consistent with the two proteins occupying overlapping binding sites in the transposon ends. Finally, we make use of the end mutations to test the idea that H-NS exerts its impact on Tn5 transposition in vivo by binding directly to the transpososome. Consistent with this possibility, we show that two different end mutations reduce the sensitivity of the Tn5 system to H-NS regulation. Conclusions H-NS typically regulates cellular functions through its potent transcriptional repressor function. Work presented here provides support for an alternative mechanism of H-NS-based regulation, and adds to our understanding of how bacterial transposition can be regulated. PMID:22503096

  20. Enzymatic large-scale synthesis of MUC6-Tn glycoconjugates for antitumor vaccination.

    PubMed

    Freire, Teresa; Lo-Man, Richard; Piller, Friedrich; Piller, Véronique; Leclerc, Claude; Bay, Sylvie

    2006-05-01

    In cancer, mucins are aberrantly O-glycosylated, and consequently, they express tumor-associated antigens such as the Tn determinant (alpha-GalNAc-O-Ser/Thr). As compared with normal tissues, they also exhibit a different pattern of expression. In particular, MUC6, which is normally expressed only in gastric tissues, has been detected in intestinal, pulmonary, colorectal, and breast carcinomas. Recently, we have shown that the MCF7 breast cancer cell line expresses MUC6-Tn glycoproteins in vivo. Cancer-associated mucins show antigenic differences from normal mucins, and as such, they may be used as potential targets for immunotherapy. To develop anticancer vaccines based on the Tn antigen, we prepared several MUC6-Tn glycoconjugates. To this end, we performed the GalNAc enzymatic transfer to two recombinant MUC6 proteins expressed in Escherichia coli, using UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts), which catalyze in vivo the Tn antigen synthesis. We used either a mixture of ppGalNAc-Ts from MCF7 breast cancer cell extracts or a recombinant ppGalNAc-T1. In both cases, we achieved the synthesis of MUC6-Tn glycoconjugates at a semi-preparative scale (mg amounts). These glycoproteins displayed a high level of Tn antigens, although the overall density depends on both enzyme source and protein acceptor. These MUC6-Tn glycoconjugates were recognized by two anti-Tn monoclonal antibodies that are specific to human cancer cells. Moreover, the MUC6-Tn glycoconjugate glycosylated using MCF7 extracts as the ppGalNAc-T source was able to induce immunoglobulin G (IgG) antibodies that recognized a human tumor cell line. In conclusion, the large-scaled production of MUC6 with tumor-relevant glycoforms holds considerable promise for developing effective anticancer vaccines, and further studies of their immunological properties are warranted. PMID:16449349

  1. Spectroscopic and ITC study of the conformational change upon Ca{sup 2+}-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle

    SciTech Connect

    Yumoto, Fumiaki [Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan); Department of Physiology II, Jikei University School of Medicine, Tokyo (Japan); Tanaka, Hiroyuki [Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido (Japan); Nagata, Koji; Miyauchi, Yumiko; Miyakawa, Takuya [Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan); Ojima, Takao [Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido (Japan); Tanokura, Masaru [Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)], E-mail: amtanok@mail.ecc.u-tokyo.ac.jp

    2008-04-25

    Akazara scallop (Chlamys nipponensis akazara) troponin C (TnC) of striated adductor muscle binds only one Ca{sup 2+} ion at the C-terminal EF-hand motif (Site IV), but it works as the Ca{sup 2+}-dependent regulator in adductor muscle contraction. In addition, the scallop troponin (Tn) has been thought to regulate muscle contraction via activating mechanisms that involve the region spanning from the TnC C-lobe (C-lobe) binding site to the inhibitory region of the TnI, and no alternative binding of the TnI C-terminal region to TnC because of no similarity between second TnC-binding regions of vertebrate and the scallop TnIs. To clarify the Ca{sup 2+}-regulatory mechanism of muscle contraction by scallop Tn, we have analyzed the Ca{sup 2+}-binding properties of the complex of TnC C-lobe and TnI peptide, and their interaction using isothermal titration microcalorimetry, nuclear magnetic resonance, circular dichroism, and gel filtration chromatography. The results showed that single Ca{sup 2+}-binding to the Site IV leads to a structural transition not only in Site IV but also Site III through the structural network in the C-lobe of scallop TnC. We therefore assumed that the effect of Ca{sup 2+}-binding must lead to a change in the interaction mode between the C-lobe of TnC and the TnI peptide. The change should be the first event of the transmission of Ca{sup 2+} signal to TnI in Tn ternary complex.

  2. 75 FR 33765 - Foreign-Trade Zone 77-Memphis, TN, Application for Subzone, Delta Faucet Company (Faucets...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-15

    ...Zone 77--Memphis, TN, Application for Subzone, Delta Faucet Company (Faucets); Jackson, TN An application...subzone status for the faucet manufacturing facility of Delta Faucet Company (Delta), located in Jackson, Tennessee. The...

  3. 78 FR 25403 - Proposed Amendment of Class E Airspace; Dayton, TN, Establishment of Class E Airspace; Cleveland...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ...Revocation of Class E Airspace; Bradley Memorial Hospital, Cleveland, TN AGENCY: Federal...airport. Information regarding Bradley Memorial Hospital would be added to the Cleveland...text as well as its listing as Bradley Memorial Hospital, Cleveland, TN, to...

  4. Tn5401, a new class II transposable element from Bacillus thuringiensis.

    PubMed Central

    Baum, J A

    1994-01-01

    A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, in the same orientation, encoding proteins of 36 kDa (306 residues) and 116 kDa (1,005 residues) and 53-bp terminal inverted repeats. The deduced amino acid sequence for the 36-kDa protein shows 24% sequence identity with the TnpI recombinase of the B. thuringiensis transposon Tn4430, a member of the phage integrase family of site-specific recombinases. The deduced amino acid sequence for the 116-kDa protein shows 42% sequence identity with the transposase of Tn3 but only 28% identity with the TnpA transposase of Tn4430. Two small open reading frames of unknown function, designated orf1 (85 residues) and orf2 (74 residues), were also identified. Southern blot analysis indicated that Tn5401, in contrast to Tn4430, is not commonly found among different subspecies of B. thuringiensis and is not typically associated with known insecticidal crystal protein genes. Transposition was studied with B. thuringiensis by using plasmid pEG922, a temperature-sensitive shuttle vector containing Tn5401. Tn5401 transposed to both chromosomal and plasmid target sites but displayed an apparent preference for plasmid sites. Transposition was replicative and resulted in the generation of a 5-bp duplication at the target site. Transcriptional start sites within Tn5401 were mapped by primer extension analysis. Two promoters, designated PL and PR, direct the transcription of orf1-orf2 and tnpI-tnpA, respectively, and are negatively regulated by TnpI. Sequence comparison of the promoter regions of Tn5401 and Tn4430 suggests that the conserved sequence element ATGTCCRCTAAY mediates TnpI binding and cointegrate resolution. The same element is contained within the 53-bp terminal inverted repeats, thus accounting for their unusual lengths and suggesting an additional role for TnpI in regulating Tn5401 transposition. Images PMID:7514590

  5. Tn6188 - A Novel Transposon in Listeria monocytogenes Responsible for Tolerance to Benzalkonium Chloride

    PubMed Central

    Muhterem-Uyar, Meryem; Zaiser, Andreas; Stessl, Beatrix; Guinane, Caitriona M.; Cotter, Paul D.; Wagner, Martin; Schmitz-Esser, Stephan

    2013-01-01

    Controlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S.) aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC) as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR) transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs) (28.5 ± 4.7 mg/l) than strains without Tn6188 (14 ± 3.2 mg/l). Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that ?qacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L. monocytogenes strains. PMID:24098567

  6. Regional Lead Agents and County Coordinators 2011 RESPONSIBILITY NAME COUNTY

    E-print Network

    Bolding, M. Chad

    Lead Millie Davenport HGIC County Coordinator Matt Burns Pickens County Coordinator Marty Watt Anderson Coordinator TBD Hampton #12;REGION 9 Regional Lead Bob Guinn Beaufort County Coordinator Alta Mae Marvin

  7. Multicopy integration of mini-Tn7 transposons into selected chromosomal sites of a Salmonella vaccine strain

    PubMed Central

    Roos, Karen; Werner, Esther; Loessner, Holger

    2015-01-01

    Chromosomal integration of expression modules for transgenes is an important aspect for the development of novel Salmonella vectors. Mini-Tn7 transposons have been used for the insertion of one such module into the chromosomal site attTn7, present only once in most Gram-negative bacteria. However, integration of multiple mini-Tn7 copies might be suitable for expression of appropriate amounts of antigen or combination of different modules. Here we demonstrate that integration of a 9.6?kb mini-Tn7 harbouring the luciferase luxCDABE (lux) occurs at the natural attTn7 site and simultaneously other locations of the Salmonella chromosome, which were engineered using ?-Red recombinase to contain one or two additional artificial attTn7 sites (a-attTn7). Multicopy integration even at closely spaced attTn7 sites was unexpected in light of the previously reported distance-dependent Tn7 target immunity. Integration of multiple copies of a mini-Tn7 containing a gfp cassette resulted in increasing green fluorescence of bacteria. Stable consecutive integration of two mini-Tn7 encoding lacZ and lux was achieved by initial transposition of lacZ-mini-Tn7, subsequent chromosomal insertion of a-attTn7 and a second round of transposition with lux-mini-Tn7. Mini-Tn7 thus constitutes a versatile method for multicopy integration of expression cassettes into the chromosome of Salmonella and possibly other bacteria. PMID:25488129

  8. MIDDLE TN RES & ED CTR UNIV OF TN BULL TEST DR. F. DAVID KIRKPATRICK BOX 160 2011-12 SR BULLS EXT AN SCI-BEEF DEPT

    E-print Network

    Tennessee, University of

    TEST SITE: MIDDLE TN RES & ED CTR UNIV OF TN BULL TEST DR. F. DAVID KIRKPATRICK BOX 160 2011-12 SR.39 76 3.35 96 1294 100 .43 50.0 4.8 33.2 3.87 14.3 F 26 BC EAGLE EYE 110-7 9/ 1/10 210 1468 2.07 3.71 83.8 37.1 3.13 12.7 28 TC ABERDEEN 759 9/ 5/10 0174 1325 3.39 4.26 96 3.16 91 1164 90 .22 51.7 5.7 33.4 3

  9. Pontotoc County Government Summer

    E-print Network

    Balasundaram, Balabhaskar "Baski"

    Pontotoc County Government Summer Youth Internship Program June 17 - 21, 2013 Sponsored By Government Summer Youth Internship Program June 17-21, 2013 Who: Youth ages 14-19 who attend a Pontotoc 24, 2013. What: Learn About YOUR Pontotoc County Government. Youth will spend time in each

  10. Continuing Education for County Officials The duties and responsibilities of county government

    E-print Network

    Continuing Education for County Officials The duties and responsibilities of county government, the V.G. Young Institute of County Government provides continuing education programs for local government officials, including county judges and commissioners, county treasurers, county and district

  11. Tn4563 transposition in Streptomyces coelicolor and its application to isolation of new morphological mutants.

    PubMed Central

    Schauer, A T; Nelson, A D; Daniel, J B

    1991-01-01

    The Tn3-like transposon Tn4556 (and its derivatives Tn4560 and Tn4563) has been used for insertion mapping of genetic loci cloned on plasmids, but it has been difficult to obtain chromosomal insertions, largely because of the lack of a strong selection against transposon donor molecules. In this communication, we report two efficient selection techniques for transposition and their use in the isolation of chromosomal insertion mutations. A number of independent Streptomyces coelicolor morphological mutants (bld and whi) were obtained. Two of the bld mutations were mapped to locations on the chromosome by SCP1-mediated conjugation; at least one mutation, bld-5m1, appears to define a novel locus involved in control of S. coelicolor morphogenesis and antibiotic production. Images PMID:1650343

  12. Biochemical and functional characterization of the Tn-specific lectin from Salvia sclarea seeds.

    PubMed

    Medeiros, A; Bianchi, S; Calvete, J J; Balter, H; Bay, S; Robles, A; Cantacuzène, D; Nimtz, M; Alzari, P M; Osinaga, E

    2000-03-01

    SSL, the lectin isolated from Salvia sclarea seeds, recognizes the Tn antigen (GalNAcalpha-O-Ser/Thr), a specific marker of many human carcinomas. Two-dimensional electrophoresis, amino-acid and amino-sugar analysis, and MALDI-TOF MS showed that SSL is an acidic (pI 5.5), 60-61-kDa dimeric glycoprotein composed of apparently identical subunits linked by a single disulfide bond. The apparent molecular mass of SSL in solution determined by equilibrium sedimentation analytical ultracentrifugation was 59 +/- 9 kDa. This value did not change in the pH range 2.5-8.5, indicating that SSL does not associate into higher order structures. Tandem mass spectrometry and methylation analysis of N-glycans released from SSL by hydrazinolysis indicated that SSL possesses 2-3 glycosylation sites occupied with the typical plant glycans Manalpha1-6[(Manalpha1-3)(Xylbeta1-2)]Manbeta1-4 -GlcNAcbeta1-4(Fucalp ha1-3)GlcNAc and [(Manalpha1-3/6)(Xylbeta1-2)]Manbeta1-4-GlcNAcbeta1 -4(Fucalpha1-3)Glc NAc. The influence of adjacent Tn structures on the binding of two Tn-specific lectins (SSL and the isolectin B4 from Vicia villosa) and an anti-Tn monoclonal antibody (mAb 83D4) was evaluated using synthetic Tn glycopeptides. The binding of both lectins to the synthetic Tn glycopeptides was independent of the density of Tn structures. On the other hand, mAb 83D4 only reacted with glycopeptides displaying two or three consecutive Tn structures. PMID:10691981

  13. Expression of slow skeletal TnI in adult mouse hearts confers metabolic protection to ischemia

    Microsoft Academic Search

    Kayla M. Pound; Grace M. Arteaga; Mathew Fasano; Tanganyika Wilder; Susan K. Fischer; Chad M. Warren; Adam R. Wende; Mariam Farjah; E. Dale Abel; R. John Solaro; E. Douglas Lewandowski

    2011-01-01

    Changes in metabolic and myofilament phenotypes coincide in developing hearts. Posttranslational modification of sarcomere proteins influences contractility, affecting the energetic cost of contraction. However, metabolic adaptations to sarcomeric phenotypes are not well understood, particularly during pathophysiological stress. This study explored metabolic adaptations to expression of the fetal, slow skeletal muscle troponin I (ssTnI). Hearts expressing ssTnI exhibited no significant ATP

  14. Mutagenesis of Alcaligenes eutrophus by insertion of the drug-resistance transposon Tn5

    Microsoft Academic Search

    Sheela Srivastava; Marlies Urban; Bärbel Friedrich

    1982-01-01

    Drug-resistance element Tn5 coding for kanamycin resistance was used for mutagenesis of Alcaligenes eutrophus strain H16. The vehicle for introducing Tn5 into A. eutrophus was plasmid pJB4JI harboured by Escherichia coli. Kanamycin-resistant transconjugants occurred at a frequency of approximately 5×10-8. One third of the transconjugants exhibited other plasmid-coded resistances such as gentamycin and spectinomycin. However, the latter markers were not

  15. Single molecule study of heterotypic interactions between mucins possessing the Tn cancer antigen.

    PubMed

    Haugstad, Kristin E; Stokke, Bjørn T; Brewer, C Fred; Gerken, Thomas A; Sletmoen, Marit

    2015-05-01

    Mucins are linear, heavily O-glycosylated proteins with physiological roles that include cell signaling, cell adhesion, inflammation, immune response and tumorgenesis. Cancer-associated mucins often differ from normal mucins by presenting truncated carbohydrate chains. Characterization of the binding properties of mucins with truncated carbohydrate side chains could thus prove relevant for understanding their role in cancer mechanisms such as metastasis and recognition by the immune system. In this work, heterotypic interactions of model mucins that possess the Tn (GalNAc?Thr/Ser) and T (Gal?1-3GalNAc?Thr/Ser) cancer antigens derived from porcine submaxillary mucin (PSM) were studied using atomic force microscopy. PSM possessing only the Tn antigen (Tn-PSM) was found to bind to PSM analogs possessing a combination of T, Tn and STn antigens as well as biosynthetic analogs of the core 1 blood group A tetrasaccharide (GalNAc?1-3[Fuc?1-2] Gal?1-3GalNAc?Ser/Thr). The rupture forces for the heterotypic interactions ranged from 18- to 31 pN at a force-loading rate of ?0.5 nN/s. The thermally averaged distance from the bound complex to the transition state (x?) was estimated to be in the range 0.37-0.87 nm for the first barrier of the Bell Evans analysis and within 0.34-0.64 nm based on a lifetime analysis. These findings reveal that the binding strength and energy landscape for heterotypic interactions of Tn-PSM with the above mucins, resemble homotypic interactions of Tn-PSM. This suggests common carbohydrate epitope interactions for the Tn cancer antigen with the above mucin analogs, a finding that may be important to the role of the Tn antigen in cancer cells. PMID:25527429

  16. Bridging Brown County Case Study Bridging Brown County Case Study

    E-print Network

    Minnesota, University of

    Bridging Brown County Case Study Bridging Brown County Case Study Connecting Communities Capacity 4 Collaboration 4 Networking 4 Community Vitality 4 Social Capital #12;Bridging Brown County Case, in 1998 a tornado caused severe damage in parts of the county. As Katie assisted groups and coordinated

  17. 76 FR 12306 - Revisions to the California State Implementation Plan, Imperial County, Kern County, and Ventura...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ...County, and Ventura County; Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District...

  18. 78 FR 7993 - Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-05

    ...Docket No. 11-ASO-28] Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation...SUMMARY: This action amends Class D and Class E airspace and removes Class E...proposed rulemaking (SNPRM) to amend Class D and Class E airspace in the...

  19. Variation on a theme; an overview of the Tn916/Tn1545 family of mobile genetic elements in the oral and nasopharyngeal streptococci

    PubMed Central

    Santoro, Francesco; Vianna, Morgana E.; Roberts, Adam P.

    2014-01-01

    The oral and nasopharyngeal streptococci are a major part of the normal microbiota in humans. Most human associated streptococci are considered commensals, however, a small number of them are pathogenic, causing a wide range of diseases including oral infections such as dental caries and periodontitis and diseases at other body sites including sinusitis and endocarditis, and in the case of Streptococcus pneumoniae, meningitis. Both phenotypic and sequence based studies have shown that the human associated streptococci from the mouth and nasopharynx harbor a large number of antibiotic resistance genes and these are often located on mobile genetic elements (MGEs) known as conjugative transposons or integrative and conjugative elements of the Tn916/Tn1545 family. These MGEs are responsible for the spread of the resistance genes between streptococci and also between streptococci and other bacteria. In this review we describe the resistances conferred by, and the genetic variations between the many different Tn916-like elements found in recent studies of oral and nasopharyngeal streptococci and show that Tn916-like elements are important mediators of antibiotic resistance genes within this genus. We will also discuss the role of the oral environment and how this is conducive to the transfer of these elements and discuss the contribution of both transformation and conjugation on the transfer and evolution of these elements in different streptococci. PMID:25368607

  20. A Class 1 Integron Present in a Human Commensal Has a Hybrid Transposition Module Compared to Tn402: Evidence of Interaction with Mobile DNA from Natural Environments? †

    PubMed Central

    Labbate, M.; Chowdhury, P. Roy; Stokes, H. W.

    2008-01-01

    In a survey of class 1 integrons from human stools, an unusual class 1 integron from a strain of Enterobacter cloacae was isolated and characterized in detail. Sequence analysis of a fosmid containing the class 1 integron revealed a complex set of transposons which included two Tn402-like transposons. One of these transposons, Tn6007, included a class 1 integron with two non-antibiotic-resistance-type gene cassettes and a complete transposition module. This tni module is a hybrid with a boundary within the res site compared to Tn402, implying that a site-specific recombination event generated either Tn6007 or Tn402. The second Tn402-like transposon, Tn6008, possesses neither a mer operon nor an integron, and most of its tni module has been deleted. Tn6007, Tn6008, and the 2,478 bases between them, collectively designated Tn6006, have transposed into a Tn5036/Tn3926-like transposon as a single unit. Tn6006, Tn6007, and Tn6008 could all transpose as discrete entities. Database analysis also revealed that a version of Tn6008 was present in the genome of Xanthomonas campestris pv. vesicatoria. Overall, the E. cloacae isolate further demonstrated that functional class 1 integrons/transposons are probably common in bacterial communities and have the potential to add substantially to the problem of multidrug-resistant nosocomial infections. PMID:18502858

  1. A newly generated functional antibody identifies Tn antigen as a novel determinant in the cancer cell-lymphatic endothelium interaction.

    PubMed

    Danussi, Carla; Coslovi, Anna; Campa, Cristiana; Mucignat, Maria T; Spessotto, Paola; Uggeri, Fulvio; Paoletti, Sergio; Colombatti, Alfonso

    2009-10-01

    Malignant transformation of epithelial cells is frequently associated with the alteration of glycosylation pathways. Tn is a common tumor-associated carbohydrate antigen present in 90% of human carcinomas and its expression correlates with metastatic potential and poor prognosis. Despite its relevance, the functional role of Tn in tumor biology has not been firmly established probably for the lack of appropriate experimental tools. Our aims were to produce highly reactive monoclonal antibodies against Tn making use of synthetically produced Tn and to test their usefulness for in vivo imaging as well as to define their potential functional activity in tumor cell spread. We immunized mice with Tn clustered on cationized BSA and screened the positive hybridomas with Tn-biotinylated alginate. Enzyme-linked immuno sorbent assay and immunofluorescence assays revealed that the most reactive anti-Tn IgM mAb (2154F12A4) selectively recognized Tn on the MCF7 breast cancer cell line since its binding to the cell membrane was completely abolished by preincubation with purified Tn. Importantly, QDot 800-conjugated mAb injected in MCF7-tumor bearing mice specifically bound to primary tumor lesions as well as to metastases in lymph nodes. In addition, this mAb was able to inhibit cancer cell adhesion to lymphatic endothelium suggesting a novel involvement of Tn in the lymphatic dissemination of cancer cells and hypothesizing future applications in inhibiting lymphatic metastases. PMID:19528665

  2. Spectroscopic and ITC study of the conformational change upon Ca 2+-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle

    Microsoft Academic Search

    Fumiaki Yumoto; Hiroyuki Tanaka; Koji Nagata; Yumiko Miyauchi; Takuya Miyakawa; Takao Ojima; Masaru Tanokura

    2008-01-01

    Akazara scallop (Chlamys nipponensis akazara) troponin C (TnC) of striated adductor muscle binds only one Ca2+ ion at the C-terminal EF-hand motif (Site IV), but it works as the Ca2+-dependent regulator in adductor muscle contraction. In addition, the scallop troponin (Tn) has been thought to regulate muscle contraction via activating mechanisms that involve the region spanning from the TnC C-lobe

  3. Construction of Tn5 lac, a Transposon That Fuses lacZ Expression to Exogenous Promoters, and Its Introduction into Myxococcus xanthus

    Microsoft Academic Search

    Lee Kroos; Dale Kaiser

    1984-01-01

    A promoterless trp-lac fusion fragment was inserted near one end of the bacterial transposon Tn5 in the correct orientation to fuse lacZ gene expression to promoters outside Tn5. The resulting transposon, Tn5 lac, retains the kanamycin-resistance gene of Tn5 and transposes in Escherichia coli at 6% the frequency of Tn5 to many different sites in a bacteriophage lambda target. Expression

  4. Tn5 transposase and tagmentation procedures for massively scaled sequencing projects

    PubMed Central

    Picelli, Simone; Björklund, Åsa K.; Reinius, Björn; Sagasser, Sven; Winberg, Gösta

    2014-01-01

    Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications. PMID:25079858

  5. Conjugative transfer of Tn916 in Enterococcus faecalis: trans activation of homologous transposons.

    PubMed Central

    Flannagan, S E; Clewell, D B

    1991-01-01

    Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harboring Tn916 delta E (a derivative in which erm is substituted for tet) on the chromosome and Tn916 on a nonconjugative plasmid. When these strains were used as donors, the two transposons were able to transfer independently; however, they were found to transfer and become coestablished in the recipient up to 50% of the time. In contrast, cotransfer of a plasmid marker located outside the transposon occurred at a frequency of no greater than 0.5%. Separate experiments showed that mobilization of the nonconjugative plasmids pAM401 and pVA749 by chromosome-borne copies of Tn916 occurred only at low frequencies (generally less than 2% cotransfer). The data imply that the initiation of transposition of Tn916 results in a trans activation that is specific for homologous transposons present in the same cell. PMID:1657880

  6. Genetic analysis of Tn916-like elements conferring tetracycline resistance in clinical isolates of Clostridium difficile.

    PubMed

    Dong, Danfeng; Chen, Xu; Jiang, Cen; Zhang, Lihua; Cai, Gang; Han, Lizhong; Wang, Xuefeng; Mao, Enqiang; Peng, Yibing

    2014-01-01

    As an important clinically relevant pathogen, Clostridium difficile has a high multidrug resistance rate. Conjugative transposons play a vital role in its resistance phenotype. In the present study, 34 tetracycline-resistant clinical isolates of C. difficile were studied to detect tetracycline resistance genes and the presence of transposons. Thirty-two isolates were found to harbour Tn916-like elements carrying the tet(M) resistance gene, of which only one copy existed in the genome by Southern blot analysis. To characterise the genetic organisation of the Tn916-like elements, overlap PCR assays were performed with nine primer pairs, revealing three types of elements designated T1 to T3. The prevalent element T1 lacking PCRA (ORF23 to ORF21) and PCRB (ORF21 to ORF20) products, present in the epidemic ST37 clone, was further analysed by genome walking PCR in the left and right end sequences of the novel Tn916-like element. A gene coding for an FtsK/SpoIIIE family protein was found to replace the ORF24 to ORF21 region in Tn916. Moreover, the element could hardly conjugate between cells by filter mating experiments. These findings suggest that the dissemination of Tn916-like elements in epidemic ST37 strains in China was likely to have been conferred by clonal spread, signifying the importance of future surveillance and characterisation of conjugative transposons. PMID:24176599

  7. Tn5 transposase and tagmentation procedures for massively scaled sequencing projects.

    PubMed

    Picelli, Simone; Björklund, Asa K; Reinius, Björn; Sagasser, Sven; Winberg, Gösta; Sandberg, Rickard

    2014-12-01

    Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications. PMID:25079858

  8. [Variations of annual load of TN and TP in the deep bay watershed, Shenzhen].

    PubMed

    Zhao, Chen-Chen; Zhang, Shi-Yan; Mao, Xian-Zhong

    2014-11-01

    The empirical coefficient of sewage disposal, export coefficient model and mean concentration method were respectively used to estimate variations of annual load TN and TP from Shenzhen and Hong Kong areas in the Deep Bay Watershed from 1986 to 2011. The results showed that, the annual average loads of TN and TP were 10 388.2 t, 10 727.9 t, 10 937.3 t, and 2 694.5 t, 1 929.2 t, 1388.7 t, respectively in the whole watershed during three periods, 80s, 90s and years after 2000. With the rapid development of society, economy and the urbanization, annual pollution loading of TN and TP in Shenzhen area showed an obviously increase, 4373.6 t and 195.9 t, by 261.0% and 64.2% for point source, and 1067.2 t and 151.0 t, by 63.4% and 84.9% for non-point source, respectively. Non-point source with high pollution load was mainly caused by the expanding of land for construction and roads. The contribution ratios of TN and TP from Shenzhen area increased from 42.4% and 27.0% to 85.1% and 75.2%. Annual loads of TN and TP in Hong Kong area decreased 3 028.5 t and 1 031.5 t, by 66.3% and 79.0% reduced. PMID:25639083

  9. The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat.

    PubMed Central

    Parent, R; Roy, P H

    1992-01-01

    We have sequenced the gene coding for the chloramphenicol acetyltransferase of Tn2424 of plasmid NR79. This gene codes for a protein of 23,500 Da, and the derived protein sequence is similar to those of the chromosomal chloramphenicol acetyltransferases of Agrobacterium tumefaciens and Pseudomonas aeruginosa and of unidentified open reading frames, which may encode chloramphenicol acetyltransferases, adjacent to the ermG macrolide-lincosamide-streptogramin resistance gene of Bacillus sphaericus and the vgb virginiamycin resistance gene of Staphylococcus aureus. Weaker similarity to the LacA (thiogalactoside acetyltransferase) and CysE (serine acetyltransferase) proteins of Escherichia coli and the NodL protein of Rhizobium leguminosarum is also observed. There is no significant similarity to any other chloramphenicol acetyltransferase genes, such as that of Tn9. The Tn2424 cat gene is part of a 4.5-kb region which also contains the aacA1a aminoglycoside-6'-N-acetyltransferase gene; Tn2424 is similar to Tn21 except for the presence of this region. Sequences flanking the cat gene are typical of those flanking other genes inserted into pVS1-derived "integrons" by a site-specific recombinational mechanism. Images PMID:1314803

  10. The SF1 helicase encoded by the archaeal plasmid pTN2 of Thermococcus nautili.

    PubMed

    Béguin, Pierre; Baron, Bruno; Gill, Sukhvinder; Charpin, Nicole; Forterre, Patrick

    2014-07-01

    We expressed, purified, and characterized the helicase encoded by ORF1 of the Thermococcus nautili pTN2 plasmid (Soler et al. Nucl Acids Res 38, 5088-5104, 2010). The enzyme, which belongs to the SF1 family of helicases, possesses NTPase activity, with a strong preference for ATP and GTP as compared to CTP and TTP; dATP was also a substrate. Triphosphatase activity was strongly stimulated by single-stranded DNA and, to a lesser extent, by double-stranded DNA. Unwinding of duplexes comprising a fluorescent oligonucleotide was monitored by fluorescence polarization spectroscopy and by polyacrylamide gel electrophoresis. As observed for enzymes of the same family, pTN2 helicase displays a strong preference for duplexes comprising a 3' single-stranded extension and proceeds from the 3' to the 5' end of the loading strand. Under the conditions of the in vitro assay, pTN2 helicase did not appear to be recycled, but stayed bound to single-stranded DNA, which explains why high concentrations of enzyme are required to unwind long stretches of duplex DNA. The helicase enhances the synthesis of double-stranded DNA by pTN2 primase and by T. nautili PolB polymerase primed by pTN2 primase but it did not enhance synthesis by Taq DNA polymerase. PMID:24889120

  11. Distribution of the catabolic transposon Tn5271 in a groundwater bioremediation system.

    PubMed Central

    Wyndham, R C; Nakatsu, C; Peel, M; Cashore, A; Ng, J; Szilagyi, F

    1994-01-01

    The distribution of Tn5271-related DNA sequences in samples of groundwater and a groundwater bioremediation system at the Hyde Park (Niagara Falls, N.Y.) chemical landfill site was investigated. PCR amplification of target sequences within the cha genes of Tn5271 revealed similar sequences in the groundwater community and in samples from the sequencing batch reactors treating that groundwater. Cell dilution combined with PCR amplification indicated that cha sequences were carried in about 1 of 10 culturable bacteria from the treatment system. Characterization of isolates involved in chlorobenzoate and toluene biodegradation in the treatment system indicated that two phenotypic clusters, Alcaligenes faecalis type 2 and CDC group IVC-2, contained all of the Tn5271 probe-positive isolates from the community. These two groups differed phenotypically from recipient groups isolated following horizontal transfer of pBRC60 (Tn5271) in pristine freshwater microcosms. A genetic rearrangement in Tn5271 attributable to the intramolecular transposition of the flanking element IS1071R was detected in an isolate from the treatment system. Comparison of the structure of the intramolecular transposition derivative from groundwater isolate OCC13(pBRC13) with a laboratory-derived intramolecular transposition derivative of pBRC60 revealed similarities. The rearrangement was shown to increase the stability of the plasmid under starvation conditions. Images PMID:8117095

  12. Homology of a transferable tetracycline resistance determinant of Clostridium difficile with Streptococcus (Enterococcus) faecalis transposon Tn916.

    PubMed Central

    Hächler, H; Kayser, F H; Berger-Bächi, B

    1987-01-01

    In several tetracycline-resistant (Tetr) Clostridium difficile strains, homology with the Tn916 part of plasmid pAM120 DNA was observed. This 15-kilobase transposon, carrying a Tetr determinant, was originally found in Streptococcus (Enterococcus) faecalis. Hybridization experiments revealed that at least six of seven HincII fragments of Tn916, representing greater than 95% of its length, showed homology with DNA of Tetr C. difficile strains. Therefore, a close relationship of the C. difficile Tetr-determining element with the entire Tn916 transposon can be assumed, although differences were observed concerning the number of HindIII cleavage sites within the transposon. In addition to strong hybridization of Tetr determinants of C. difficile with Tn916, weak signals were detected when DNA of Tets C. difficile was hybridized with Tn916. These weak signals could be attributed to a single internal HincII fragment of Tn916. Images PMID:2821887

  13. Quantitative assessment of Tn antigen in breast tissue micro-arrays using CdSe aqueous quantum dots.

    PubMed

    Au, Giang H T; Mejias, Linette; Swami, Vanlila K; Brooks, Ari D; Shih, Wan Y; Shih, Wei-Heng

    2014-03-01

    In this study, we examined the use of CdSe aqueous quantum dots (AQDs) each conjugated to three streptavidin as a fluorescent label to image Tn antigen expression in various breast tissues via a sandwich staining procedure where the primary monoclonal anti-Tn antibody was bound to the Tn antigen on the tissue, a biotin-labeled secondary antibody was bound to the primary anti-Tn antibody, and finally the streptavidin-conjugated AQDs were bound to the biotin on the secondary antibody. We evaluated the AQD staining of Tn antigen on tissue microarrays consisting of 395 cores from 115 cases including three tumor cores and one normal-tissue core from each breast cancer case and three tumor cores from each benign case. The results indicated AQD-Tn staining was positive in more than 90% of the cells in the cancer cores but not the cells in the normal-tissue cores and the benign tumor cores. As a result, AQD-Tn staining exhibited 95% sensitivity and 90% specificity in differentiating breast cancer against normal breast tissues and benign breast conditions. These results were better than the 90% sensitivity and 80% specificity exhibited by the corresponding horse radish peroxidase (HRP) staining using the same antibodies on the same tissues and those of previous studies that used different fluorescent labels to image Tn antigen. In addition to sensitivity and specificity, the current AQD-Tn staining with a definitive threshold was quantitative. PMID:24411673

  14. Isolation of behavioral mutants of Azospirillum brasilense by using Tn5 lacZ.

    PubMed Central

    van Rhijn, P; Vanstockem, M; Vanderleyden, J; De Mot, R

    1990-01-01

    Tn5 lacZ mutants were generated with Azospirillum brasilense 7030 by mating that strain with Escherichia coli strains carrying suicide plasmid pCIB100 or pCIB110. Kanamycin-resistant Azospirillum colonies were obtained with a maximum frequency of 10(-6) per recipient cell. The potential of Tn5 lacZ for random transposon mutagenesis coupled to transcription analysis in A. brasilense 7030 was demonstrated. Sixty percent of all Kmr A. brasilense 7030 mutants expressed beta-galactosidase activity. Mutants affected in motility (Fla-) and general chemotaxis (Che-) were identified. Chromosomal insertions of Tn5 lacZ are involved, except for two Che- mutants. The latter che loci reside on a 90-megadalton plasmid. Expression of an acidic protein (Mr, 110,000) was abolished in these mutants. Images PMID:2160221

  15. 75 FR 24944 - Adequacy Status of the Milwaukee-Racine, Door County, Manitowoc County, and Sheboygan County...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-06

    ...Manitowoc County, and Sheboygan County, Wisconsin Areas Submitted 8-Hour Ozone Redesignation...Manitowoc County, and Sheboygan County, Wisconsin ozone nonattainment areas are adequate...transportation conformity determinations. Wisconsin submitted a redesignation request...

  16. Novel Tn4371-ICE like element in Ralstonia pickettii and Genome mining for comparative elements

    PubMed Central

    2009-01-01

    Background Integrative Conjugative Elements (ICEs) are important factors in the plasticity of microbial genomes. An element related to the ICE Tn4371 was discovered during a bioinformatic search of the Ralstonia pickettii 12J genome. This element was analysed and further searches carried out for additional elements. A PCR method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight Ralstonia pickettii and Ralstonia insidiosa strains were analysed for the presence of the element. Results Comparative sequence analysis of bacterial genomes has revealed the presence of a number of uncharacterised Tn4371-like ICEs in the genomes of several ? and ?- Proteobacteria. These elements vary in size, GC content, putative function and have a mosaic-like structure of plasmid- and phage-like sequences which is typical of Tn4371-like ICEs. These elements were found after a through search of the GenBank database. The elements, which are found in Ralstonia, Delftia, Acidovorax, Bordetella, Comamonas, Acidovorax, Congregibacter, Shewanella, Pseudomonas Stenotrophomonas, Thioalkalivibrio sp. HL-EbGR7, Polaromonas, Burkholderia and Diaphorobacter sp. share a common scaffold. A PCR method was designed (based on the Tn4371- like element detected in the Ralstonia pickettii 12J genome) to detect and characterise new elements of this type. Conclusion All elements found in this study possess a common scaffold of core genes but contain different accessory genes. A new uniform nomenclature is suggested for ICEs of the Tn4371 family. Two novel Tn4371-like ICE were discovered and characterised, using the novel PCR method described in two different isolates of Ralstonia pickettii from laboratory purified water. PMID:19941653

  17. Synergistic template-free synthesis of dsDNA by Thermococcus nautili primase PolpTN2, DNA polymerase PolB, and pTN2 helicase.

    PubMed

    Béguin, Pierre; Gill, Sukhvinder; Charpin, Nicole; Forterre, Patrick

    2015-01-01

    A combination of three enzymes from the hyperthermophilic archaeon Thermococcus nautili, DNA primase PolpTN2, DNA polymerase PolB, and pTN2 DNA helicase, was found to synthesize up to 300-400 ng/µl dsDNA from deoxynucleotide triphosphates in less than 30 min in the absence of added template DNA and oligonucleotide primer. The reaction did not occur below 64 °C. No synthesis was observed if PolpTN2 or PolB were left out; helicase was not essential but accelerated the reaction. The DNA synthesized consisted of highly reiterated palindromic sequences reaching up to more that 10 kb. Sequence analysis of three independent reaction products synthesized at different temperatures showed that the palindromes shared a common pentanucleotide core, suggesting that random nucleic acid fragments were not responsible for priming the reaction. When enzymes were added sequentially, preincubation with primase plus helicase followed by PolB led to a shorter delay before the onset of the reaction as compared to preincubation with PolB plus helicase followed by primase. This suggests that the primase generates seeds that are subsequently amplified and elongated in synergy with PolB by a mechanism involving hairpin formation and slippage synthesis. PMID:25420601

  18. 4. Photocopy of photograph showing the corn crib as it ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Photocopy of photograph showing the corn crib as it looked on May 21, 1936. This structure was also used as a gear shed. Immediately to the right of the shed is Hettie Walker and an unidentified family member or friend. The original photograph was taken by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8091. - Walker Family Farm, Corn Crib, Gatlinburg, Sevier County, TN

  19. Budgeting in Texas Counties, 1931-1940.

    E-print Network

    Bradshaw, H. C.

    1941-01-01

    the ~arions associations of county officers, particularly the county judges and commissioners, the county auditors, and the county dmlcs. CONTENTS Introduction 5 Purpose of Study 6 Procedure 6 Legal Provisions 9 The Budget Form 11 Number of Budgets...

  20. Jasper County Comprehensive Plan Adoption Draft

    E-print Network

    Jasper County Comprehensive Plan Adoption Draft Comprehensive Plan Jasper County, IN #12;#12;Table Mandate.......................................vii Jasper County's Fulfillment of the Mandate of Major Needs for Jasper County ...............5 Additional Opportunities

  1. Snohomish County Biodiesel Project

    SciTech Connect

    Terrill Chang; Deanna Carveth

    2010-02-01

    Snohomish County in western Washington State began converting its vehicle fleet to use a blend of biodiesel and petroleum diesel in 2005. As prices for biodiesel rose due to increased demand for this cleaner-burning fuel, Snohomish County looked to its farmers to ���¢��������grow���¢������� this fuel locally. Suitable seed crops that can be crushed to extract oil for use as biodiesel feedstock include canola, mustard, and camelina. The residue, or mash, has high value as an animal feed. County farmers began with 52 acres of canola and mustard crops in 2006, increasing to 250 acres and 356 tons in 2008. In 2009, this number decreased to about 150 acres and 300 tons due to increased price for mustard seed.

  2. County Waterford Image Archive

    NSDL National Science Digital Library

    Using volunteer help and a locally written image management system, the Waterford County Museum in Ireland has created this collection of close to 3,000 historical images that document Waterford County and its parishes from the late 1890s to the present. The Web interface to the collection includes a number of helpful features - for those familiar with Waterford county and its place and family names, there is a search box, but for those less familiar there are several ways to browse the collection, by selecting photographer, location, image format, or subjects from drop down lists, as well as selecting parishes from a map to see the pictures associated with each. A photo of the week is posted on the site, and visitors can sign up to receive an RSS feed or, alternatively, an email message when new images are added. There are also easy ways to submit photographs for inclusion in the archive, and for locals to contribute identifying information.

  3. (Dengue Fever reported in Osceola County) OSCEOLA COUNTY HEALTH DEPARTMENT

    E-print Network

    Watson, Craig A.

    (Dengue Fever reported in Osceola County) OSCEOLA COUNTY HEALTH DEPARTMENT ISSUES PUBLIC HEALTH of two cases of dengue fever in Osceola County. Dengue is one of several mosquito-borne illnesses was not acquired locally. Symptoms of dengue fever vary according to the age of the patient. Infants and young

  4. Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome

    SciTech Connect

    Shoemaker, N.B.; Getty, C.; Gardner, J.F.; Salyers, A.A.

    1986-03-01

    The gene for resistance to erythromycin and clindamycin, which is carried on the conjugative Bacteroides plasmid, pBF4, has been shown previously to be part of an element (Tn4351) that transposes in Escherichia coli. The authors have now introduced Tn4351 into Bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range IncP plasmid R751 (R751::Tn4351) and (ii) pSS-2, a chimeric plasmid which contains 33 kilobases of pBF4 (including Tn4351) cloned into the IncQ plasmid RSF1010 and which is mobilized by R751. When E. coli HB101, carrying either R751::Tn4351 or R751 and pSS-2, was mated with B. uniformis under aerobic conditions, Em/sup r/ transconjugants were detected at a frequency of 10 /sup -6/ to 10/sup -5/ (R751::Tn4351) or 10/sup -8/ to 10/sup -6/ (R751 and pSS-2). In matings involving pSS-2, all Em/sup r/ transconjugants contained simple insertions of Tn4351 in the chromosome, whereas in matings involving R751::Tn4351, about half of the Em/sup r/ transconjugants had R751 cointegrated with Tn4351 in the chromosome. Of the Em/sup r/ transconjugants, 13% were auxotrophs. Bacteroides spp. which had R751 cointegrated with Tn4351 in the chromosome did not transfer R751 or Tn4351 to E. coli HB101 or to isogenic B. uniformis, nor did the integrated R751 mobilize pE5-2, an E. coli-Bacteroides shuttle vector that contains a transfer origin that is recognized by R751.

  5. Purchasing in Texas Counties.

    E-print Network

    Hervey, E. J.; Bradshaw, H. C.

    1944-01-01

    8 r3' L \\, & #5, CnLpL"; 3' --%I k? TEXAS AGRICULTURAL EXPERIMENT STATION A. B. CONNER, Director College Station, Texas BULLETIN NO. 653 JULY 1944 PURCHASING IN TEXAS COUNTIES H. C. BRADSEAW and E. J, HERVEY Division of Farm and Ranch... Economics 00.: .*- AGRICULTURAL AND MECHANICAL COLLEGE OF TEXAS GIBB GILCHRIST, President D-21-744-4500 The use of good purchasing procedures in a number of Texas counties is reducing the cost price of materials, supplies and equipment by 15 to 20 per...

  6. Petroleum County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Petroleum County Secondary Data Analysis July 23, 2012 1 Community Health Data, MT Dept American Diabetes Association (2012) Region 3 (South Central) ­ Judith Basin, Fergus, Petroleum* #12; Petroleum County Secondary Data Analysis July 23, 2012 2 Socioeconomic Measures1

  7. Geothermal element, Imperial County, California

    NASA Astrophysics Data System (ADS)

    Edmunds, S.; Sullivan, J.; Goldsmith, M.

    1977-11-01

    Research methodology a brief history of geothermal development in the county, and a general history and physical characteristics of the county are described. A framework of the county master plan for geothermal development, its implications, and short and long range planning procedures was also considered.

  8. Orange County Florida Master Gardener

    E-print Network

    Jawitz, James W.

    Orange County Florida Master Gardener OOffffiicciiaall VVoolluunntteeeerr GGuuiiddeebbooookk #12;iii Welcome to the Orange County Florida Master Gardener Volunteer Program. You have been selected to study as a Master Educator of landscape and garden knowledge. The Orange County Florida Master Gardener

  9. Wetlands Regulatory Assistance Program ERDC TN-WRAP-12-1 August 2012

    E-print Network

    US Army Corps of Engineers

    Wetlands Regulatory Assistance Program ERDC TN-WRAP-12-1 August 2012 Approved for public release; distribution is unlimited. Updating Regional Supplements to the Corps of Engineers Wetland Delineation Manual by Jacob F. Berkowitz PURPOSE: Regional supplements to the Corps of Engineers Wetland Delineation Manual

  10. 78 FR 6257 - Proposed Amendment of Class C Airspace; Nashville International Airport, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ...RIN 2120-AA66 Proposed Amendment of Class C Airspace; Nashville International Airport...Nashville International Airport, TN, Class C airspace area by removing a cutout from the...proposing this action to return the Class C airspace area to the standard...

  11. ERDC/EL TN-11-1 Flood Risk Management: Insights from

    E-print Network

    US Army Corps of Engineers

    ERDC/EL TN-11-1 March 2011 Flood Risk Management: Insights from an Expert Modeling Process by M. D preparedness planning that harmonizes efforts of implementing agencies and stakeholders. Risk management are essential for effective risk management policy. Formal (versus ad hoc) analyses of risk manager and stake

  12. Transposon mutagenesis by Tn4560 and applications with avermectin-producing Streptomyces avermitilis.

    PubMed Central

    Ikeda, H; Takada, Y; Pang, C H; Tanaka, H; Omura, S

    1993-01-01

    The Tn3-like Streptomyces transposon Tn4560 was used to mutagenize Streptomyces avermitilis, the producer of anthelmintic avermectins and the cell growth inhibitor oligomycin. Tn4560 transposed in this strain from a temperature-sensitive plasmid to the chromosome and from the chromosome to a plasmid with an apparent frequency of about 10(-4) to 10(-3) at both 30 and 39 degrees C. Auxotrophic and antibiotic nonproducing mutations were, however, obtained only with cultures that were kept at 37 or 39 degrees C. About 0.1% of the transposon inserts obtained at 39 degrees C caused auxotrophy or abolished antibiotic production. The sites of insertion into the S. avermitilis chromosome were mapped. Chromosomal DNA fragments containing Tn4560 insertions in antibiotic production genes were cloned onto a Streptomyces plasmid with temperature-sensitive replication and used to transport transposon mutations to other strains, using homologous recombination. This technique was used to construct an avermectin production strain that no longer makes the toxic oligomycin. PMID:8384619

  13. Preclinical Class 1 Integron with a Complete Tn402-Like Transposition Module?

    PubMed Central

    Sajjad, Ammara; Holley, Marita P.; Labbate, Maurizio; Stokes, H. W.; Gillings, Michael R.

    2011-01-01

    The presence of integrons was assessed in gut bacteria isolated from wild-caught prawns. A pseudomonad was recovered that contained a Tn402-like class 1 integron with a complete transposition module and two gene cassettes. One cassette was identical to a previously described cassette from a chromosomal class 3 integron in Delftia tsuruhatensis. PMID:21037292

  14. Tn: The Number of n-Node Labeled Trees Theorem 106 (Cayley's formula (1889)) a

    E-print Network

    Lyuu, Yuh-Dauh

    1889-01-01

    > 1 from now on. aArthur Cayley (1821­1895). c2013 Prof. Yuh-Dauh Lyuu, National Taiwan University by the multinomial theorem (p. 66). c2013 Prof. Yuh-Dauh Lyuu, National Taiwan University Page 702 #12;Arthur CayleyTn: The Number of n-Node Labeled Trees Theorem 106 (Cayley's formula (1889)) a The number of trees

  15. MODALITY INDEPENDENT ELASTOGRAPHY Michael I. Miga, Vanderbilt University, Dept. of Biomedical Engineering, Nashville, TN

    E-print Network

    Miga, Michael I.

    .e. the similarity between images is maximized, the process has converged and an elasticity image is created. Figure Engineering, Nashville, TN Email: Michael.I.Miga@vanderbilt.edu Introduction: Alternative imaging modalities.) of tissue have become of great interest to the medical imaging community. The clinical goal is to develop

  16. Earlier detection of myocardial infarction by an improved cardiac TnI assay

    Microsoft Academic Search

    Estelle Le Moal; Isabelle Giuliani; Jean-Pierre Bertinchant; Anne Polge; Catherine Larue; Sylvie Villard-Saussine

    2007-01-01

    Objectives:The aim of this study was to establish the diagnostic sensitivity of combinations of well-selected monoclonal antibodies (mAbs) against cardiac troponin I (cTnI) to allow an earlier rule-in of acute coronary syndrome (ACS) patients.

  17. The effect of Tn916 insertions on contour-clamped homogeneous electrophoresis patterns of Enterococcus faecalis.

    PubMed Central

    Thal, L A; Silverman, J; Donabedian, S; Zervos, M J

    1997-01-01

    Contour-clamped homogeneous gel electrophoresis has increasingly been used and has generally been considered the method of choice for the delineation of enterococcial strains. It has been suggested that the contour-clamped homogeneous electric field (CHEF) electrophoresis digestion patterns of genomic DNA indicate the relatedness of strains when SmaI patterns differ by six or fewer bands. To evaluate the potential reasons for the diversity of bands among clonally related isolates, we studied plasmid-free Enterococcus faecalis FA2-2 and derivatives with transposon Tn916 (encoding for tetracycline resistance) insertions on the chromosome. We obtained derivatives with seven different Tn916 insertion sites and up to two copies of Tn916 on the chromosome, resulting in differences of one to seven bands by CHEF electrophoresis; eight different patterns were observed. With Tn916 insertions, there was either (i) loss of a band(s) with the generation of a new band(s), (ii) the generation of a new band(s) only, or (iii) the loss of a band(s) with no new visible band(s). These results indicate that strains can have up to seven different SmaI bands by CHEF electrophoresis and still be closely related. PMID:9157164

  18. Transposon mutagenesis of the anaerobic commensal, Bacteroides fragilis, using the EZ::TN5 transposome.

    PubMed

    Veeranagouda, Yaligara; Husain, Fasahath; Wexler, Hannah M

    2012-08-01

    Genetic analysis of Bacteroides fragilis (BF) is hindered because of the lack of efficient transposon mutagenesis methods. Here, we describe a simple method for transposon mutagenesis using EZ::TN5, a commercially available system that we optimized for use in BF638R. The modified EZ::TN5 transposon contains an Escherichia coli conditional origin of replication, a kanamycin resistance gene for E. coli, an erythromycin resistance gene for BF , and 19 basepair transposase recognition sequences on either ends. Electroporation of the transposome (transposon-transposase complex) into BF638R yielded 3.2 ± 0.35 × 10(3) CFU ?g(-1) of transposon DNA. Modification of the transposon by the BF638R restriction/modification system increased transposition efficiency sixfold. Electroporation of the EZ::TN5 transposome results in a single-copy insertion of the transposon evenly distributed across the genome of BF638R and can be used to construct a BF638R transposon library. The transposon was also effective in mutating a BF clinical isolate and a strain of the related species, Bacteroides thetaiotaomicron. The EZ::TN5-based mutagenesis described here is more efficient than other transposon mutagenesis approaches previously reported for BF. PMID:22639975

  19. ERDC/TN APCRP-EA-22 Management of Half Moon Lake,

    E-print Network

    US Army Corps of Engineers

    ERDC/TN APCRP-EA-22 May 2010 Management of Half Moon Lake, Wisconsin, for Improved Native Submersed that result in poor light penetration and limited colonizable macrophyte habitat. Half Moon Lake Half Moon Lake is shallow and supports a native submersed macrophyte community, poor underwater light

  20. ERDC/TN APCRP-EA-23 Effects of Nutrients, Salinity, and pH on

    E-print Network

    US Army Corps of Engineers

    ERDC/TN APCRP-EA-23 July 2010 Effects of Nutrients, Salinity, and pH on Salvinia molesta (Mitchell (nitrogen and phosphorus) concentra- tions, pH, and salinity on giant salvinia growth. BACKGROUND: Giant factors including temperature, nutrient availability, salinity, and pH may limit the distribution

  1. Tropos1 at the Age of Eight: On-going Research at FBK, UniTN and UT

    E-print Network

    .giorgini,jm}@disi.unitn.it 2 University of Toronto, Toronto, Canada 3 Fondazione Bruno Kessler, Povo, Trento, Italy {perini on the project at Fondazione Bruno Kessler (FBK), the University of Trento (UniTN) and the University of Toronto of Toronto (hereafter UT), the University of Trento (UniTN) and the Fondazione Bruno Kessler (FBK) known

  2. Fusion Power Associates Annual Meeting and Symposium, Oak Ridge, TN, 4-5 December 2007 Gary Johnson

    E-print Network

    1 Fusion Power Associates Annual Meeting and Symposium, Oak Ridge, TN, 4-5 December 2007 Gary Era Status of ITERStatus of ITER #12;2 Fusion Power Associates Annual Meeting and Symposium, Oak Ridge · Technical Status · Summary #12;3 Fusion Power Associates Annual Meeting and Symposium, Oak Ridge, TN, 4

  3. FATE OF TN5 MUTANTS OF ROOT GROWTH-INHIBITING PSEUDOMONAS SP. IN INTACT SOIL-CORE MICROCOSMS

    EPA Science Inventory

    Transposon Tn5 mutants of a wheat root growth-inhibiting nonfluorescent Pseudomonas sp. were inoculated into intact soil-core microcosms to determine the utility of intact soil cores for evaluating the fate and transport of microorganisms in agricultural ecosystems. ransposon Tn5...

  4. Please submit the following documents to the Dashew Center no later than three months before the current TN status expires.

    E-print Network

    Williams, Gary A.

    : ______________________________________________________________________________________________ Family First Middle Sex Male Marital Status: Single [Please provide dependent(s) Female Married before the current TN status expires. List of Documents for TN 1) Biographical Information Form (see next current immigration status Copies of all I-20s, EADs, DS-2019s, H-1B approval notices, Form I-94 printout

  5. Westchester County Employers Survey.

    ERIC Educational Resources Information Center

    Lee, Marcia M.

    The Westchester County Employers Survey was done in May 2003 in order learn more about employee training needs, how they accomplished these needs, and how it would be possible for the Westchester Community College to fulfill these needs. Out of the 639 surveys sent, 145 were returned in a satisfactory format, which produces a 22.6% return rate.…

  6. Washington County Comprehensive plan

    E-print Network

    Washington County Comprehensive plan · 2010 4. makIng The Case for PlannIng 17 Collective vs. Individual Inventory & Assessment - Bodies of Water: Lakes, Rivers, and Streams 81 Inventory & Assessment - Forests and Parkland 82 Inventory & Assessment - Wetlands 82 Inventory & Assessment - Agricultural/Farmland 82

  7. STATE/COUNTY BORDERS

    EPA Science Inventory

    This data is available for the entire region. The level of detail is primarily suited for region/state/county/basin sized maps. The borders are not accurate enough for small areas like sub-basins and site areas. We use this coverage for general backgrounds and borders only. T...

  8. 7 CFR 7.11 - County committee members.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...county executive director, other employee of the county committee, or the county agricultural extension agent for the county. If the county agricultural extension agent is not selected as secretary to the county committee, that person...

  9. 78 FR 43827 - Irish Potatoes Grown in Modoc and Siskiyou Counties, California, and in All Counties in Oregon...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-22

    ...Siskiyou Counties, California, and in All Counties in Oregon, Except Malheur County...Siskiyou Counties, California, and in all counties in Oregon, except Malheur County...at: http://www.regulations.gov. All comments submitted in response to this...

  10. County Business Patterns 1995

    NSDL National Science Digital Library

    The US Census Bureau has made available the state publications for the 1995 version of its annual County Business Patterns 1995 series (Adobe Acrobat [.pdf] format only). Data available include employment, payroll, and number of establishments by Standard Industrial Code category. Establishments are further broken down by nine employment size classes ranging from 1-4 to 1,000 or more. The US volume is forthcoming. Complete 1993 and 1994 (also .pdf format) series are available at the site.

  11. High-resolution structure of a new Tn antigen-binding lectin from Vatairea macrocarpa and a comparative analysis of Tn-binding legume lectins.

    PubMed

    Sousa, Bruno Lopes; Silva Filho, José Caetano; Kumar, Prashant; Pereira, Ronniery Ilário; ?yskowski, Andrzej; Rocha, Bruno Anderson Matias; Delatorre, Plínio; Bezerra, Gustavo Arruda; Nagano, Celso Shiniti; Gruber, Karl; Cavada, Benildo Sousa

    2015-02-01

    Plant lectins have been studied as histological markers and promising antineoplastic molecules for a long time, and structural characterization of different lectins bound to specific cancer epitopes has been carried out successfully. The crystal structures of Vatairea macrocarpa (VML) seed lectin in complex with GalNAc-?-O-Ser (Tn antigen) and GalNAc have been determined at the resolution of 1.4? and 1.7?, respectively. Molecular docking analysis of this new structure and other Tn-binding legume lectins to O-mucin fragments differently decorated with this antigen provides a comparative binding profile among these proteins, stressing that subtle alterations that may not influence monosaccharide binding can, nonetheless, directly impact the ability of these lectins to recognize naturally occurring antigens. In addition to the specific biological effects of VML, the structural and binding similarities between it and other lectins commonly used as histological markers (e.g., VVLB4 and SBA) strongly suggest VML as a candidate tool for cancer research. PMID:25499445

  12. 75 FR 26709 - Clarke County Water Supply Project, Clarke County, IA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ...Conservation Service Clarke County Water Supply Project, Clarke County, IA AGENCY...prepared for the Clarke County Water Supply Project, Clarke County, Iowa...information effectively lowered the water supply project target from 3.2 mgd to...

  13. 77 FR 73005 - Revisions to the California State Implementation Plan, Imperial County, Placer County, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-07

    ...Placer County, and Ventura County Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD)...

  14. 77 FR 72968 - Revisions to the California State Implementation Plan, for Imperial County, Placer County and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-07

    ...Placer County and Ventura County Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD)...

  15. 78 FR 29202 - Environmental Impact Statement: Grand Forks County, North Dakota and Polk County, Minnesota

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-17

    ...Statement: Grand Forks County, North Dakota and Polk County, Minnesota AGENCY...project in Grand Forks County, North Dakota and Polk County, Minnesota. FOR...Federal Highway Administration, North Dakota Division Office, 1471...

  16. 75 FR 49016 - County of Greenville, S.C.-Acquisition Exemption-Greenville County Economic Development Corporation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ...Exemption--Greenville County Economic Development Corporation The County of...acquire from Greenville County Economic Development Corporation (GCEDC) approximately...See Greenville County Economic Development...

  17. Tn 1721 -encoded resolvase: structure of the tnpR gene and its in vitro functions

    Microsoft Academic Search

    Peter Rogowsky; Riidiger Schmitt

    1985-01-01

    Summary  A 760 base pair nucleotide sequence of transposon Tn1721 containing the resolvase (tnpR) gene has been determined. A 186 triplet open reading frame was assigned to tnpR, and the allocation of-35 and-10 promoter boxes was supported by mapping transcription initiation at 70 base pairs upstream\\u000a of tnpR. Expression of tnpR under tac promoter control generated sufficient resolvase protein for enzyme

  18. Modeling in-situ uranium(VI) bioreduction at Oak Ridge, TN

    Microsoft Academic Search

    J. Luo; F. Weber; O. A. Cirpka; W. Wu; J. L. Nyman; J. M. Carley; P. M. Jardine; C. S. Criddle; P. K. Kitanidis

    2005-01-01

    A travel-time based reactive-transport model is developed to simulate an in-situ bioremediation experiment for demonstrating enhanced bioreduction of uranium(VI) at Oak Ridge, TN. The space to travel-time transformation is obtained from a conservative-tracer test. Reactive transport is solved numerically in time and travel-time coordinates. Reaction kinetics include aquatic equilibrium geochemistry, uranium sorption and precipitation, and the microbial reduction of nitrate,

  19. Design analysis report for the TN-WHC cask and transportation system

    SciTech Connect

    Brisbin, S.A., Fluor Daniel Hanford

    1997-02-13

    This document presents the evaluation of the Spent Nuclear Fuel Cask and Transportation System. The system design was developed by Transnuclear, Inc. and its team members NAC International, Nelson Manufacturing, Precision Components Corporation, and Numatec, Inc. The cask is designated the TN-WHC cask. This report describes the design features and presents preliminary analyses performed to size critical dimensions of the system while meeting the requirements of the performance specification.

  20. ROLE OF NA+ IN TRANSPORT OF HG2+ AND INDUCTION OF THE TN21 "MER" OPERON

    EPA Science Inventory

    The effects of sodium ions on the uptake of Hg 2 + and induction of the TN21 mer operon were studied using E. coli HMS174 harboring the reporter plasmids pRB28 and pOS14. lasmid pRB28 carries merRT' and pOS14 carries merRTPC of the mer operon, both cloned upstream of a promoterle...

  1. Tn924, a chromosome-borne transposon encoding high-level gentamicin resistance in Enterococcus faecalis.

    PubMed Central

    Thal, L A; Chow, J W; Clewell, D B; Zervos, M J

    1994-01-01

    Enterococcus faecalis SF350 is a clinical isolate from Winnipeg, Canada, with high-level (MIC > 2,000 micrograms/ml) gentamicin resistance. The genetic determinant for gentamicin resistance was located on the chromosome of SF350 and could be mobilized by a coresident conjugative plasmid, pYN120. Genetic and physical analyses showed that the gentamicin resistance determinant was located on a 27-kb transposable element which was designated Tn924. Images PMID:8067754

  2. Transposon Tn 21 encodes a RecA -independent site-specific integration system

    Microsoft Academic Search

    Eduardo Martinez; Fernando de la Cruz

    1988-01-01

    The IncW plasmid R388 and the DNA region of Tn21 containing the Smr and the Sur genes are capable of RecA-independent recombination. This recombination occurs at a relatively high frequency (up to 10-4 recombinants per recipient molecule) and results in integration of the two plasmids. No detectable repeats are formed in the process. The crossover points have been confined to

  3. Morphological and Functional Diversity of Ectomycorrhizal Fungi on Roan Mountain (NC\\/TN)

    Microsoft Academic Search

    Claire Bird; Coleman McCleneghan

    2005-01-01

    A comparison of ectomycorrhizal morphotypes and hypogeous fungi (truffles and false-truffles) in northern hardwood and spruce-fir forests on Roan Mountain (NC\\/TN) was performed to increase our knowledge of the fungal communities in the Southern Appalachian high elevation forests. These forests are home to an endangered subspecies and mycophagist, the Carolina northern flying squirrel (Glaucomys sabrinus coloratus), as well as the

  4. Tn5-induced lipopolysaccharide mutations in Bordetella pertussis that affect outer membrane function

    Microsoft Academic Search

    M. L. Turcotte; Denis Martin; Bernard R. Brodeur; Mark S. Peppler

    1997-01-01

    An LPSB-specific mAb was used to screen for ten TnS insertion mutants of Bordetella pertussis which have LPS which is phenotypically distinct from either wild-type LPSAB or LPSB. Silver-stained SDS-PAGE gels showed nine different LPS phenotypes, six of which contain two clinically undocumented LPS bands, designated lntA and lntB based on their proximity to the LPSA and LPSB bands, respectively.

  5. Mutations that affect Tn5 insertion into pBR322: importance of local DNA supercoiling.

    PubMed Central

    Lodge, J K; Berg, D E

    1990-01-01

    The major hot spot of transposon Tn5 insertion in plasmid pBR322 (hot spot I) is in the promoter for the tetracycline resistance gene (tet). We made a series of pBR322 derivatives with mutations in and around this promoter and assayed their effects on insertion of Tn5 into hot spot I. Those mutations which reduced transcription from the tet promoter also reduced the frequency of insertion into hot spot I. Transcription and translation of tet are thought to cause the formation of paired domains of negative and positive supercoiling in pBR322. An amber codon in tet, 345 base pairs from hot spot I, decreases the negative supercoiling of the DNA segment containing hot spot I because it terminates translation of tet prematurely. We report here that this amber mutation also reduces insertion into hot spot I. These results suggest that the ability of Tn5 to insert into its major hot spot in pBR322 depends directly on negative supercoiling of the target DNA. PMID:2170333

  6. Cell cycle dependent TN-C promoter activity determined by live cell imaging.

    PubMed

    Halter, Michael; Sisan, Daniel R; Chalfoun, Joe; Stottrup, Benjamin L; Cardone, Antonio; Dima, Alden A; Tona, Alessandro; Plant, Anne L; Elliott, John T

    2011-03-01

    The extracellular matrix protein tenascin-C plays a critical role in development, wound healing, and cancer progression, but how it is controlled and how it exerts its physiological responses remain unclear. By quantifying the behavior of live cells with phase contrast and fluorescence microscopy, the dynamic regulation of TN-C promoter activity is examined. We employ an NIH 3T3 cell line stably transfected with the TN-C promoter ligated to the gene sequence for destabilized green fluorescent protein (GFP). Fully automated image analysis routines, validated by comparison with data derived from manual segmentation and tracking of single cells, are used to quantify changes in the cellular GFP in hundreds of individual cells throughout their cell cycle during live cell imaging experiments lasting 62 h. We find that individual cells vary substantially in their expression patterns over the cell cycle, but that on average TN-C promoter activity increases during the last 40% of the cell cycle. We also find that the increase in promoter activity is proportional to the activity earlier in the cell cycle. This work illustrates the application of live cell microscopy and automated image analysis of a promoter-driven GFP reporter cell line to identify subtle gene regulatory mechanisms that are difficult to uncover using population averaged measurements. PMID:22045641

  7. EEO Employment Data Allegheny County

    E-print Network

    Sibille, Etienne

    EEO Employment Data for Allegheny County and the Pittsburgh region February 2008 University Center................................................................................................................. 2 Employment Summary by EEO Occupation Group............................................................................... 3 Employment by Detail Census Occupation Category

  8. Digital Atlas of Texas Counties

    NSDL National Science Digital Library

    Created by the Center for Geosptial Technology at Texas Tech University, this digital atlas is a fantastic find. It brings together information about all of Texas's counties, including satellite relief maps and data sets about the roads, rivers, lakes, and other features in each area. Visitors can use the interactive map to click on a county of interest, or use the drop down menu to select a region. Once users select a county, they can download information about it for future use. The site also contains a number of Featured Links to resources from the U.S. Census Bureau, the Texas Association of Counties, and several tourism agencies.

  9. Spectroscopic and ITC study of the conformational change upon Ca{sup 2+}-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle

    Microsoft Academic Search

    Fumiaki Yumoto; Hiroyuki Tanaka; Koji Nagata; Yumiko Miyauchi; Takuya Miyakawa; Takao Ojima; Masaru Tanokura

    2008-01-01

    Akazara scallop (Chlamys nipponensis akazara) troponin C (TnC) of striated adductor muscle binds only one Ca{sup 2+} ion at the C-terminal EF-hand motif (Site IV), but it works as the Ca{sup 2+}-dependent regulator in adductor muscle contraction. In addition, the scallop troponin (Tn) has been thought to regulate muscle contraction via activating mechanisms that involve the region spanning from the

  10. The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

    PubMed Central

    De Silva, Ravindra A.; Appulage, Dananjaya K.; Pietraszkiewicz, Halina; Bobbitt, Kevin R.; Media, Joe; Shaw, JiaJiu; Valeriote, Fred A.

    2012-01-01

    The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; ?-D-GalpNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera. PMID:22297566

  11. 77 FR 73978 - Foreign-Trade Zone 148-Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-12

    ...Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing Authority), Opening of Comment...Tennessee, with authority to manufacture carbon fiber for export and oxidized polyacrylonitrile fiber (Board Order 1868, 77 FR 69435,...

  12. Taiwan Nantou County earthquake 0327 Taiwan Nantou County earthquake

    E-print Network

    Taiwan Nantou County earthquake 20130327 1 #12;0327 Taiwan Nantou County earthquake Source, Intensity 5 #12;I II III IV V VI VII Intensity Shake map of the March 27 Earthquake The peak ground and Technology Center for Disaster Reduction (NCDR) #12;Earthquake Response and Evacuation are a Part of Students

  13. Ca(2+)-regulatory function of the inhibitory peptide region of cardiac troponin I is aided by the C-terminus of cardiac troponin T: Effects of familial hypertrophic cardiomyopathy mutations cTnI R145G and cTnT R278C, alone and in combination, on filament sliding.

    PubMed

    Brunet, Nicolas M; Chase, P Bryant; Mihajlovi?, Goran; Schoffstall, Brenda

    2014-06-15

    Investigations of cardiomyopathy mutations in Ca(2+) regulatory proteins troponin and tropomyosin provide crucial information about cardiac disease mechanisms, and also provide insights into functional domains in the affected polypeptides. Hypertrophic cardiomyopathy-associated mutations TnI R145G, located within the inhibitory peptide (Ip) of human cardiac troponin I (hcTnI), and TnT R278C, located immediately C-terminal to the IT arm in human cardiac troponin T (hcTnT), share some remarkable features: structurally, biochemically, and pathologically. Using bioinformatics, we find compelling evidence that TnI and TnT, and more specifically the affected regions of hcTnI and hcTnT, may be related not just structurally but also evolutionarily. To test for functional interactions of these mutations on Ca(2+)-regulation, we generated and characterized Tn complexes containing either mutation alone, or both mutations simultaneously. The most important results from in vitro motility assays (varying [Ca(2+)], temperature or HMM density) show that the TnT mutant "rescued" some deleterious effects of the TnI mutant at high Ca(2+), but exacerbated the loss of function, i.e., switching off the actomyosin interaction, at low Ca(2+). Taken together, our experimental results suggest that the C-terminus of cTnT aids Ca(2+)-regulatory function of cTnI Ip within the troponin complex. PMID:24418317

  14. The bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi

    Microsoft Academic Search

    Clive Lo; Kiichi Adachi; Jeffrey R. Shuster; John E. Hamer; Lisbeth Hamer

    2003-01-01

    TAGKO is a Tn7-based transposition system for genome wide mutagenesis in filamentous fungi. The effects of transposon insertion on the expression of TAGKO alleles were examined in Magnaporthe grisea and Mycosphaerella graminicola. Northern analysis showed that stable, truncated transcripts were expressed in the TAGKO mutants. Mapping of the 3¢-ends of TAGKO cDNAs revealed that they all contain Tn7 end sequences,

  15. Protective action of tetramethylpyrazine phosphate against dilated cardiomyopathy in cTnTR141W transgenic mice

    Microsoft Academic Search

    Hai-ping Zhao; Dan Lü; Wei Zhang; Li Zhang; Shu-mei Wang; Chun-mei Ma; Chuan Qin; Lian-feng Zhang

    2010-01-01

    Aim:Dilated cardiomyopathy (DCM) is the most common cause of heart failure, and pharmacological intervention is not currently available. Here we investigate the effect of tetramethylpyrazine phosphate (TMPP) on the progression of DCM in the cTnTR141W transgenic mouse model.Methods:The cTnTR141W transgenic mice aged 2 months were divided into model group and TMPP group, whereas age-matched nontransgenic mice were used as wild-type

  16. The Listeria monocytogenes transposon Tn6188 provides increased tolerance to various quaternary ammonium compounds and ethidium bromide.

    PubMed

    Müller, Anneliese; Rychli, Kathrin; Zaiser, Andreas; Wieser, Cornelia; Wagner, Martin; Schmitz-Esser, Stephan

    2014-12-01

    Tolerance of the foodborne pathogen Listeria monocytogenes to sublethal concentrations of disinfectants has been frequently reported. Particularly, quaternary ammonium compounds (QACs) such as benzalkonium chloride (BC) are often used in disinfectants and also as antiseptics in food industry and hospitals. Recently, we described Tn6188, a novel transposon in L. monocytogenes harbouring the transporter QacH, a molecular mechanism leading to increased tolerance to BC. In this study, we investigated the presence of Tn6188 within the genus Listeria spp. Our screening indicates that the distribution of Tn6188 may be limited to L. monocytogenes. We confirm that QacH is responsible for the observed increase in tolerance by complementation of a qacH deletion mutant and introducing qacH in a Tn6188 negative strain. We investigated the transporter's substrate spectrum by determining minimal inhibitory concentrations (MICs) and showed that QacH also confers higher tolerance towards other QACs and ethidium bromide (EtBr). This result was supported by increased expression of qacH in the presence of the various substrates as determined by quantitative reverse transcriptase PCR (qRT-PCR). In addition, we detected expression of a Tn6188 transposase gene and circular forms of Tn6188, suggesting activity and possible transfer of this transposon. PMID:25312720

  17. POWER IN A RURAL COUNTY

    Microsoft Academic Search

    WILLIAM BEAVER; ERIC COHEN

    2004-01-01

    To more fully understand power structures in rural settings, this article investigates the power structure of an economically depressed rural county in southwestern Pennsylvania. The power structure must be viewed in the context of the economic hard times that the county has confronted. In this regard, there has been a decline in the number of local economic notables over the

  18. Richland County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau. Local Area Unemployment Statistics (LAUS). Non Seasonally Adjusted Unemployment Rate. (Retrieved, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100, Asian/Pacific Islanders, Hispanic & NonHispanic Ancestry 6 US Census Bureau (2010) 5 County Health

  19. Wheatland County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau. Local Area Unemployment Statistics (LAUS). Non Seasonally Adjusted Unemployment Rate. (Retrieved Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 173.8 182, Asian/Pacific Islanders, Hispanic & NonHispanic Ancestry 6 US Census Bureau (2010) 5 County Health

  20. Blaine County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    transportation-related, poisoning, and suffocation. Chronic Disease Burden1 County Montana Nation3,4 Stroke prevalence 3.3% 2.5% 2.6% Diabetes prevalence 9.4% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart, Pondera, Teton, Chouteau, and Cascade Chronic Disease Hospitalization Rates County Montana Stroke

  1. Roosevelt County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    transportation-related, poisoning, and suffocation. Chronic Disease Burden1 County Montana Nation3,4 Stroke prevalence 3.2% 2.5% 2.6% Diabetes prevalence 12.9% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke Per 100,000 population 1 196.5 182

  2. Valley County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    County Health Ranking, Robert Wood Johnson Foundation (2012) Leading Causes of Death County1 are assessed each year. All children's records per provider assessed are reviewed to determine if the child (binge + heavy drinking)1 24.8% 22.8% Obesity1 26.8% 21.6% Overweight1 37.8% 37.8% No Leisure

  3. Musselshell County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ) 5 County Health Ranking, Robert Wood Johnson Foundation (2012) Leading Causes of Death County1 are assessed each year. All children's records per provider assessed are reviewed to determine if the child (binge + heavy drinking)1 20.8% 22.8% Obesity1 24.2% 21.6% Overweight1 38.3% 37.8% No Leisure

  4. Date Name Location July 4 -12 Pueblo County Fair Pueblo

    E-print Network

    Ordway July 20 - 27 Eagle County Fair and Rodeo Eagle July 21 - 27 Kit Carson County Fair and Rodeo County 4-H Fair La Veta Aug. 6 - 10 Jefferson County Jeffco Fair Golden Aug. 6 - 10 La Plata County Fair

  5. Cardiac troponin I but not cardiac troponin T adheres to polysulfone dialyser membranes in an in vitro haemodialysis model: explanation for lower serum cTnI concentrations following dialysis

    PubMed Central

    Gaze, David C; Collinson, Paul O

    2014-01-01

    Background Elevated serum cardiac troponin T (cTnT) and I (cTnI) can occur in patients with chronic kidney disease. Differences in cTn concentrations between cTnT and cTnI have been reported but the mechanism of such discrepancy has not been investigated. This study investigates the clearance of cTn with the aid of an in vitro model of haemodialysis (HD). Methods Serum was obtained before and after a single session of dialysis from 53 patients receiving HD and assayed for cTnT and cTnI. An in vitro model of the dialysis process was used to investigate the mechanism of clearance of cTn during HD. Results Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD. Using an in vitro model of dialysis, it was demonstrated that cTnI is not dialysed from the vascular compartment but adheres to the dialyser membrane. Conclusions The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis. The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar. PMID:25332816

  6. Metabolite Profiling Reveals Abiotic Stress Tolerance in Tn5 Mutant of Pseudomonas putida

    PubMed Central

    Chaudhry, Vasvi; Bhatia, Anil; Bharti, Santosh Kumar; Mishra, Shashank Kumar; Chauhan, Puneet Singh; Mishra, Aradhana; Sidhu, Om Prakash; Nautiyal, Chandra Shekhar

    2015-01-01

    Pseudomonas is an efficient plant growth–promoting rhizobacteria (PGPR); however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5) mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC) and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE) and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS) pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA) on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic stress tolerant metabolites from the NBRI1108T suggest that Tn5 mutagenesis enhanced tolerance towards high temperature and drought. Tolerance to drought was further confirmed in greenhouse experiments with maize as host plant, where NBRI1108T showed relatively high biomass under drought conditions. PMID:25629312

  7. Metabolite profiling reveals abiotic stress tolerance in Tn5 mutant of Pseudomonas putida.

    PubMed

    Chaudhry, Vasvi; Bhatia, Anil; Bharti, Santosh Kumar; Mishra, Shashank Kumar; Chauhan, Puneet Singh; Mishra, Aradhana; Sidhu, Om Prakash; Nautiyal, Chandra Shekhar

    2015-01-01

    Pseudomonas is an efficient plant growth-promoting rhizobacteria (PGPR); however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5) mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC) and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE) and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS) pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA) on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic stress tolerant metabolites from the NBRI1108T suggest that Tn5 mutagenesis enhanced tolerance towards high temperature and drought. Tolerance to drought was further confirmed in greenhouse experiments with maize as host plant, where NBRI1108T showed relatively high biomass under drought conditions. PMID:25629312

  8. Tennessee Higher Education County Profiles, 2008

    ERIC Educational Resources Information Center

    Tennessee Higher Education Commission, 2008

    2008-01-01

    This report presents a localized perspective on Tennessee higher education, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; (3) number of county residents enrolled in Tennessee public institutions; and (4) number of county residents participating in the…

  9. MonroeCounty Number of volunteers 108

    E-print Network

    Watson, Craig A.

    benefits to community 59% Students and Alumni UF students from county 161 CALS students from county 16 UF fisheries; Master Gardeners Giving Recent donors residing in county 919 FY 2012 donors residing in county.9% contribution to gross regional product. Based on a 2010 UF study to the community. $1invested in agricultural

  10. The Sugar Creek zinc deposit, Jackson Co. TN -- Exploration history, geology and mineralization

    SciTech Connect

    Reinbold, G.; Moran, A.V.; Stevens, D.L. (Independence Mining Co. Inc., Reno, NV (United States))

    1993-03-01

    During the 60's and 70's zinc exploration of central TN and KY was active. The Sugar Creek Project was one of several investigated by Exxon. The discovery hole, Cu 15, was drilled in early 1973. The Sugar Creek Zinc Deposit was acquired by Independence Mining Co. in 1986 and I.M.C. has subsequently completed additional drilling, both stepout and confirmation holes. A total of 137 holes for 300,833 ft have been drilled. The Sugar Creek deposit is a typical Tennessee zinc deposit (Mississippi Valley Type) which occurs in solution collapse breccias in the Lower Ordovician, Knox Dolomite. The Knox consists of fine grained dolomite with interlayered limestones and crystalline dolomite. Only scattered residual limestone is found in the Sugar Creek area. Collapse breccias have formed which control zinc deposition and are similar to other TN Zn. deposits. At Sugar Creek the types of breccias include: a vertically exaggerated glory hole breakthrough breccia which extends to within 137 ft. of the Knox unconformity, has 500 ft. of zinc mineralization with 8 significant zinc intervals; holes with stacked zinc intervals interpreted to be sides of breakthrough breccia; and single zinc intervals in laterally positioned bedded mineral zones. A total of 99 holes were drilled in the more intense mineralized areas. The ratio of ore to non ore holes is nearly 1 to 1. The mineralization is typical M.V.T. with predominantly sphalerite and only minor occurrences of galena, fluorite, pyrite, etc.

  11. Regulatory roles of spnT, a novel gene located within transposon TnTIR.

    PubMed

    Wei, Jun-Rong; Soo, Po-Chi; Horng, Yu-Tze; Hsieh, Shang-Chen; Tsai, Yu-Huan; Swift, Simon; Withers, Helen; Williams, Paul; Lai, Hsin-Chih

    2006-09-29

    The transposon TnTIR contains spnIR quorum-sensing system regulating sliding motility and the production of nuclease, biosurfactant, and prodigiosin in Serratia marcescens. Within TnTIR, a gene named spnT is upstream of and co-transcribed with spnI. SpnT is a cytoplasmic protein and its level peaks during early stationary phase. spnT over-expression resulted in inhibition of sliding motility and synthesis of prodigiosin, and biosurfactant similar to spnR. spnT but not spnR over-expression induced cell elongation and aberrant DNA replication in S. marcescens and Escherichia coli strains. In comparison with wild-type E. coli strain, over-expression of spnT in an E. coli priA and dnaC double-mutant strain did not lead to the aberrant cell morphology phenotypes, suggesting SpnT may act through the recombination-dependent DNA replication system. As spnT over-expression inhibited swarming but not swimming motility, SpnT may indirectly function as a negative regulator of surface-dependent migration and secondary metabolite production. PMID:16899219

  12. In-house preparation of lectin panel and detection of Tn polyagglutination

    PubMed Central

    Das, Sudipta Sekhar

    2015-01-01

    Polyagglutination is a condition in which red cells are agglutinated by ABO-compatible adult human sera, but not by cord blood sera and may be acquired or inherited. Lectins are invaluable reagents in the investigation of red cells polyagglutination. We prepared in-house lectin panel and confirmed Tn polyagglutination in a pregnant lady. The lady was anemic and refused blood transfusion elsewhere due to serological discrepancy. We found ABO discrepancy and an incompatible minor cross-match in the initial investigation and suspected polyagglutination. Confirmation of polyagglutination was done using adult and cord sera. We then used the in-house lectin panels to detect the type of polyagglutination. The agglutination pattern with the various lectins was suggestive of Tn polyagglutination, which was further supported by the enzyme study. Most blood banks in India lack commercial lectin panels because of cost and procurement difficulty. Lectins play an important role in the diagnosis and differentiation of polyagglutination and immunohematological management of patient. The important and basic lectins can be prepared in-house using specific raw seeds following standardized protocol. PMID:25722587

  13. Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha

    SciTech Connect

    Chandra, T.S.; Friedrich, C.G.

    1986-05-01

    Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the inability to grow with reduced sulfur compounds (Sox/sup -/). Four classes of Sox/sup -/ mutants were obtained. Three were of different pleiotropic phenotypes: (i) unable to grow with formate, nitrate, and xanthine; (this class strongly suggested the involvement of a molybdenum cofactor in inorganic sulfur-oxidizing ability); (ii) no growth with hydrogen; (iii) slight growth with hydrogen and formate. Two plasmids, pHG41 (about 450 kilobase pairs) and pHG42 (110 kilobases), were identified in lysates of T. pantotropha. In one Sox/sup -/ mutant pHG41 could not be detected. Revertant analysis suggested that pHG41 and pHG42 were not involved in the Sox character.

  14. [Antirestriction activity of the mercury resistance nonconjugative transposon Tn5053 is controlled by the protease ClpXP].

    PubMed

    2014-09-01

    When transformed into Escherichia coli K12 strains, the mercury resistance transposon Tn5053@ exhibits high antirestriction activity against the EcoKI type I restriction and modification system. The products of the genes merR and ardD contribute to the antirestriction activity of Tn5053. The merR gene encodes the MerR protein, the transcription regulator of the mer operon genes. The ardD gene is responsible for ArdD protein synthesis and is located within the tni operon. In the following study, it was demonstrated that the antirestriction activity of the transposon Tn5053 is absent in E. coli K12 strains with the mutant genes clpX, clpP, and recA. The antirestriction effect of Tn5053 is not enhanced by 2-aminopurine. The Tn5053 antirestriction activity is not altered in E. coli K12 with the mutant dam gene; however, it is decreased in the E. coli K12 mutD. It is assumed that the activities of the MerR and ArdD proteins lead to the formation of a significant amount of unmodified DNA in the bacterial cell, causing the SOS-dependent reduction of the EcoKI (R2M2S) enzyme activity associated with ClpXP-induced proteolysis of the R-subuinit. PMID:25735133

  15. TnI-Ultra assay measurements in cancer patients: comparison with the conventional assay and clinical implication.

    PubMed

    Salvatici, Michela; Cardinale, Daniela; Botteri, Edoardo; Bagnardi, Vincenzo; Mauro, Cristian; Cassatella, Maria C; Lentati, Paola; Bottari, Fabio; Zorzino, Laura; Passerini, Rita; Cipolla, Carlo M; Sandri, Maria T

    2014-08-01

    The serial monitoring of cardiac troponin represents an effective approach for the early identification, assessment, and monitoring of chemotherapy-induced cardiac injury. Over the last few years new generations of troponin assays, referred to as sensitive and high sensitivity assays, able to detect very low concentrations of troponin, have been progressively released on different platforms. Some studies have assessed the comparability of the cTnI measurements with the new assays versus the conventional ones, but none of these in the oncological population. We compared the cTnI results determined on Stratus CS and ADVIA Centaur CP System in 70 breast cancer patients, for a total of 327 samples collected during different cycles of treatment. Correlation (Spearman = 0.732) and agreement (91.4%) between the assays were good (244 concordant negatives and 55 concordant positives), with a frequency of 8.6% discordant results among the cTnI measurements. Despite the well-known lack in the harmonization and standardization of the currently commercially available cTnI methods, we found a good clinical concordance of cTnI determination on both systems. PMID:24693994

  16. Single-site chromosomal Tn5 insertions affect the export of pectolytic and cellulolytic enzymes in Erwinia chrysanthemi EC16.

    PubMed Central

    Thurn, K K; Chatterjee, A K

    1985-01-01

    Exponentially growing cells of Erwinia chrysanthemi EC16 usually export about 98% of their pectate lyase (PL) and protease, about 40% of their polygalacturonase (PG), and about 60% of their cellulase (endoglucanase or carboxymethyl cellulase; CL). By using the R plasmid, pJB4JI (pPH1JI::Mu::Tn5), three independent Tn5 insertion mutants were obtained that exported normal levels of protease but 10% or less of PL, PG, and CL. Physical analysis revealed that single copies of Tn5 had inserted into the E. chrysanthemi chromosome, producing a similar export-defective (Out-) phenotype. The synthesis of PL, PG, and CL was not affected by the Tn5 insertions. These enzymes were released from the mutants on spheroplast formation, indicating that they were located in the periplasmic space. Tn5 insertions caused the loss of a 35-kilodalton periplasmic protein, but did not alter the outer membrane protein composition. The findings are discussed with respect to the current knowledge on protein export in gram-negative bacteria. Images PMID:3002271

  17. VERMONT COUNTY HEALTH DATA2

    EPA Science Inventory

    This datalayer contains Vermont Population and Health data describing public health (1986-2000), by county, extracted from various sources, such as; the Vermont Department of Health, the Vermont Center for Justice Research, the National Institute on Alcohol Abuse & Alcoholis...

  18. IFAS Extension Polk County Extension

    E-print Network

    Ma, Lena

    IFAS Extension Polk County Extension Community leader and citizen education on the state of our water resources Objectives To provide decision makers, community leaders, and private citizens officials (both elected and appointed) · Community leaders · Media representatives · Private citizens

  19. Fault Trace: Marin County, California

    NSDL National Science Digital Library

    This photograph shows the trace of a fault (in trench phase) as it passes beneath a barn. The trace developed during the April 18, 1906 San Francisco Earthquake. The location is the Skinner Ranch, near Olema, Marin County, California.

  20. Geothermal Development Plan: Pima County

    SciTech Connect

    White, D.H.

    1981-01-01

    Pima County is located entirely within the Basin and Range physiographic province in which geothermal resources are known to occur. Continued growth as indicated by such factors as population growth, employment and income will require large amounts of energy. It is believed that geothermal energy could provide some of the energy that will be needed. Potential users of geothermal energy within the county are identified.

  1. Lake County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    -related, poisoning, and suffocation. Chronic Disease Burden1 County Montana Nation3,4 Stroke prevalence 2.6% 2.5% 2.6% Diabetes prevalence 6.7% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.5% 4.1% 6.0% All Hospitalization Rates County Montana Stroke Per 100,000 population 1 164.0 182.2 Diabetes Per 100,000 population 1

  2. Expression and regulation of mutant forms of cardiac TnI in a reconstituted actomyosin system: Role of kinase dependent phosphorylation

    Microsoft Academic Search

    Ashwani Malhotra; Antonio Nakouzi; Joel Bowman; Peter Buttrick

    1997-01-01

    When phosphorylated, the inhibitory subunit of troponin (TnI) causes a loss in calcium sensitivity and a decrease in actomyosin ATPase. To examine this process, we bacterially expressed wild type TnI and TnI mutants in which serine 22 and 23, a putative protein kinase A (PKA) site, and threonine 143, a putative protein kinase C (PKC) site, were replaced by alanine

  3. Evolutionary relationship between Tn 21 -like elements and pBP201, a plasmid from Klebsiella pneumoniae mediating resistance to gentamicin and eight other drugs

    Microsoft Academic Search

    Friedrich Schmidt; Ingrid Klopfer-Kaul

    1984-01-01

    We have characterized pBP201 one of the plasmids from a collection of 46 strains producing adenylyltransferase ANT (2?) (Schmidt 1984). It confers resistance to sulphonamides and produces aminoglycoside adenylyltransferases AAD (3?) and ANT (2?) and ß-lactamase TEM-1. Plasmid pBP201 has a size of 24.8 kilobases (kb) and contains TnA and a Tn21-related element, Tn4000?, with deletions in mer and the

  4. Tiu Ch Chn La D n Khi Phc Sm Vo ngy 21 thng 4 nm 2011, cc y Vin nh Gi Tn Hi

    E-print Network

    hai gii pháp u thích hp, gii pháp nào tn chi phí thp hn phi c chn. Tha thun Khôi phc sm tháng 4 nm các ch tiêu sau: · · Chi phí cn thit thc hin gii pháp Mi gii pháp c ch i tha mãn mc tiêu và mc ích ca th; Mi gii pháp có ngn nga các tn thng trong tng lai t các tai nn hoc các tn thng phát sinh t sau vic

  5. Atlas of Historical County Boundaries

    NSDL National Science Digital Library

    So you'd like to know the boundaries of Washington's King County in the early 20th century? Or perhaps you'd like to know more about the shape of Nassau County in New York back in the 19th century? The Atlas of Historical County Boundaries can provide you such details. The project is housed at the William M. Scholl Center for American History and Culture at The Newberry Library in Chicago and it was completed in 2010. The Atlas includes such features as all boundary changes in states and countries, non-county areas, separate map or polygon files for every different county configuration, and other helpful features. Users can get started by clicking on different states of interest and just exploring as they see fit. It's also useful to read over the Using the Atlas area for additional assistance. Finally, visitors can also look at the Publications section for more information on the source material for these county boundaries.

  6. 75 FR 25308 - Environmental Impact Statement: Winnebago County, IL and Rock County, WI

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ...Administration Environmental Impact Statement: Winnebago County, IL and Rock County, WI AGENCY: Federal Highway Administration (FHWA...Wisconsin Route 213 and Nye School Road northwest of Beloit, Rock County, Wisconsin to the interchange of Rockton Road and...

  7. San Bernardino County Museum

    NSDL National Science Digital Library

    The San Bernardino County Museum is a regional museum with exhibits and collections in cultural and natural history. Special exhibits, the Exploration Station live animal discovery center, extensive research collections, and public programs for adults, families, students, and children are all part of the museum experience. The Museum is surrounded by citrus groves, and orange blossoms perfume the air. The Zimmerman Citrus Kiosk explores citrus agricultural history in Southern California. Death Valley Alive! is a new traveling exhibit, available for rent, where visitors can explore the natural history and culture of the Death Valley region from its geologic beginnings 1.3 billion years ago. Online exhibits include The Importance of Museum Collections, The Barstow Fossil Beds, Motherlode of the Miocene, The Etiwanda Fan, and for kids, Mimicry, a study in camouflage and adaptation. Teacher Resources include professional development and workshops, and Trading Places, where 21 hours of time spent volunteering in the Museum earns a free program for your class. Sample opportunities include writing activities for study kits, developing post-visit activities, creating a museum gallery guide, translating student materials into Spanish, or developing an independent idea. There are programs for youth, Scouts, and adults, as well as various publications available in anthropology, archeology, biology, geology and history.

  8. Iron County Extension Agent

    E-print Network

    Wasatch Counties; William A. Varga; Teresa A. Cerny; Mike Kuhns

    2002-01-01

    What traveler, driving across Utah, has not marveled at its diversity of geography, climate and vegetation? From Joshua-trees in the Mojave Desert, to alpine meadows, to pinion-juniper forests set against the red sandstone of the Colorado Plateau, it is truly a state of contrasts. Ideally, Utah landscapes should be designed to be adapted to local conditions and to enhance the natural beauty of the state. Unfortunately, most of our landscapes are patterned after those found in Europe or northeastern America. Such landscapes are difficult to maintain and require more resources, such as water and fertilizer, than adapted landscapes. While there is no simple recommendation that would be valid state-wide, localized guidelines can address the diversities of climate, soils, and other factors which affect plant growth. The purpose of this series of publications is to recommend plants and landscape management procedures for various regions of the state. This edition targets the communities of the high mountain valleys of the state (Rich, Morgan, Summit, and Wasatch counties). These valleys are characterized by cold temperatures and short growing seasons. Proper landscape design and management can help prevent plant stress and death in these conditions. It also makes landscapes easier to maintain. The first step to successful landscaping

  9. One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI.

    PubMed

    Tuteja, Satish K; Kukkar, Manil; Suri, C R; Paul, A K; Deep, Akash

    2015-04-15

    2-Aminobenzyl amine (2-ABA) functionalized graphene is proposed for the ultrasensitive immunosensing of Cardiac Troponin I (cTnI). 2-ABA was electrochemically polymerized on the graphene decorated interdigitated electrode to obtain the amine functionalized graphene (f-GN). The f-GN electrode was then modified with monoclonal anti-cTnI antibodies via Schiff reaction based chemistry. Detailed characteristics of the processes involved and the finally developed antibody conjugated f-GN interdigitated electrode have been studied. The above micro-device was used in a drain source configuration for the sensing of cTnI. A wide dynamic linear range of antigen detection (0.01-1ng/mL) is achieved with the limit of detection of 0.01ng/mL. The utility of the proposed sensing technique is demonstrated by successfully testing the antigen concentration in spiked serum samples. PMID:25460893

  10. Differential analysis of transient increases of serum cTnI in response to handling in rats

    PubMed Central

    Mikaelian, Igor; Dunn, Michael E; Mould, Diane R; Hirkaler, Gerard; Geng, Wanping; Coluccio, Denise; Nicklaus, Rosemary; Singer, Thomas; Reddy, Micaela

    2013-01-01

    Serum cardiac troponins are the key biomarkers of myocardial necrosis in humans and in preclinical species. The use of ultrasensitive assays for serum cardiac troponin I (cTnI) as a biomarker in safety studies is hampered by interindividual differences. In this study, we investigated the effect of handling procedures on serum cTnI and explored modeling and simulation approaches to mitigate the impact of these interindividual differences. Femoral-catheterized male Crl:WI(Han) rats (n = 16/group) were left undisturbed in their cages with no handling; subjected to 5 min of isoflurane/O2 anesthesia (A); or placed into a rodent restrainer followed by simulated tail vein injection (RR). Serum cTnI concentrations were assessed over a 24-h period using an ultrasensitive assay, and the study was repeated for confirmation. The mean serum cTnI concentration pre-procedure was 4.2 pg/mL, and remained stable throughout the duration of the study in the rats submitted to the A procedure. Serum cTnI concentrations increased transiently after the RR procedure with a median time to maximum concentration (Tmax), of 1 and 2 h and a mean maximum value concentration (Cmax), of 53.0 and 7.2 pg/mL in the initial and repeat studies, respectively. A population pharmacodynamic model identified interindividual, procedure- and study-specific effects on serum cTnI concentrations in rats. It is concluded that a modeling and simulation approach more appropriately describes and statistically analyzes the data obtained with this ultrasensitive assays. PMID:25505566

  11. Description of the OXA-23 ?-Lactamase Gene Located Within Tn2007 in a Clinical Isolate of Acinetobacter baumannii from Spain.

    PubMed

    Guerrero-Lozano, Inmaculada; Fernández-Cuenca, Felipe; Galán-Sánchez, Fátima; Egea, Pilar; Rodríguez-Iglesias, Manuel; Pascual, Álvaro

    2015-04-01

    A carbapenem-resistant Acinetobacter baumannii expressing blaOXA-23 was recovered from an intensive care unit patient in a third-level hospital from Spain. Genetic analysis showed the association of this carbapenemase with the transposon Tn2007 located in a plasmid of 10?kb. The isolate was classified as ST-1. This strain has shown a potential ability to displace other endemic strains in the hospital and is the first reported identification of A. baumannii carrying blaOXA-23 related to Tn2007 in Spain. PMID:25386800

  12. A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis

    PubMed Central

    Biery, Matthew C.; Stewart, Fiona J.; Stellwagen, Anne E.; Raleigh, Elisabeth A.; Craig, Nancy L.

    2000-01-01

    A robust Tn7-based in vitro transposition system is described that displays little target site selectivity, allowing the efficient recovery of many different transposon insertions in target DNAs ranging from small plasmids to cosmids to whole genomes. Two miniTn7 derivatives are described that are useful for the analysis of genes: one a derivative for making translational and transcriptional target gene fusions and the other a derivative that can generate 15 bp (5 amino acid) insertions in target DNAs (proteins). PMID:10666445

  13. 75 FR 52364 - Notice of Intent To Repatriate Cultural Items: Memphis Pink Palace Museum, Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ...and restored ceramic vessels from the Bradley site (3CT7), Crittenden County, AR...from a private individual in 1958. The Bradley site was a village or town of the late...period from A.D. 1350-1650. The Bradley site is thought to be the capital...

  14. Synthetic and Immunological Studies of 5?-N-phenylacetyl sTn to Develop Carbohydrate-Based Cancer Vaccines and to Explore the Impacts of Linkage between Carbohydrate Antigens and Carrier Proteins

    PubMed Central

    Wang, Qianli; Ekanayaka, Sandamali Amarasingha; Wu, Jian; Zhang, Junping; Guo, Zhongwu

    2009-01-01

    5?-N-Phenylacetyl sTn (sTnNPhAc), an unnatural derivative of sTn antigen expressed by many tumors, and its ?-linked protein conjugates were prepared and investigated to explore glycoconjugate cancer vaccines. sTnNPhAc?-KLH elicited a robust T cell-dependent immunity. The antiserum derived from sTnNPhAc?- or sTnNPhAc?-KLH-inoculated mice was similarly reactive to sTnNPhAc? and sTnNPhAc? but showed very little reactivity to sTn, NeuNPhAc? (2,3)GalNAc – a regioisomer of sTnNPhAc, isolated phenylacetyl group, and the linker employed to conjugate sTnNPhAc and carrier protein. It was concluded that the sTnNPhAc-elicited immunity was specific for the whole antigen rather than the phenylacetyl group or other partial structures of sTnNPhAc and that the reducing end configuration or linkage of sTnNPhAc did not affect its immunological identity. It was also concluded that a new linker designed to conjugate carbohydrates and proteins did not provoke any immune reaction and that the linker, as well as the associated new and convenient coupling strategy, can be safely used for the development of glycoconjugate vaccines. PMID:18816108

  15. Green digital signage using nanoparticle embedded narrow-gap field sequential TN-LCDs

    NASA Astrophysics Data System (ADS)

    Kobayashi, Shunsuke; Shiraishi, Yukihide; Sawai, Hiroya; Toshima, Naoki; Okita, Masaya; Takeuchi, Kiyofumi; Takatsu, Haruyoshi

    2012-03-01

    We have fabricated field sequential color (FSC)-LCDs using cells and modules of narrow-gap TN-LCDs with and without doping the nanoparticles of PCyD-ZrO2 and AF-SiO2. It is shown that the FSC-LCD exhibits a high optical efficiency of OE=4.5 that is defined as OE=[Luminance]/[W/m2]=(cd/W). This figure may provide us a good reference or to clear the Energy Star Program Version 5-3 that issues a guideline: LCD with 50 inch on the diagonal consumes the energy of 108W. Through this research it is claimed that our FSC=LCD may be a novel green digital signage.

  16. Population substructure in Cache County, Utah: the Cache County study

    PubMed Central

    2014-01-01

    Background Population stratification is a key concern for genetic association analyses. In addition, extreme homogeneity of ethnic origins of a population can make it difficult to interpret how genetic associations in that population may translate into other populations. Here we have evaluated the genetic substructure of samples from the Cache County study relative to the HapMap Reference populations and data from the Alzheimer's Disease Neuroimaging Initiative (ADNI). Results Our findings show that the Cache County study is similar in ethnic diversity to the self-reported "Whites" in the ADNI sample and less homogenous than the HapMap CEU population. Conclusions We conclude that the Cache County study is genetically representative of the general European American population in the USA and is an appropriate population for conducting broadly applicable genetic studies. PMID:25078123

  17. The intraocular dynamics of vancomycin hydrochloride ophthalmic ointment (TN-011) in rabbits.

    PubMed

    Fukuda, Masamichi; Hanazome, Isao; Sasaki, Kazuyuki

    2003-03-01

    The intraocular dynamics of 1% vancomycin hydrochloride (VCM) ophthalmic ointment (TN-011) in rabbits was investigated. The animals used in the study were 42 pigmented rabbits with normal eyes (normal group) and 17 pigmented rabbits with Bacillus subtilis-infected eyes (BS group). The infection in the BS group was induced by a method previously established by the authors. Fifty milligrams of a 1% VCM ophthalmic ointment was administered into the cul-de-sacs of the animals' eyes unilaterally in both groups. Serum, bulbar conjunctiva (only from the normal group), cornea, iris-ciliary body, and vitreous were collected 15, 30, 60, 120, and 240 min after the drug administration as samples. Drug concentration was measured by the bioassay method. In the normal group, VCM in the bulbar conjunctiva reached a maximum concentration (98.98 +/- 46.48 microg/g) at 30 min, and decreased to a level below the detection limit at 240 min. The maximum concentration of VCM in the cornea (12.04 +/- 4.73 microg/g) was observed at 30 min. VCM was not detected in the aqueous humour, iris-ciliary body, vitreous, or serum in the normal group at any point. In the BS group, the maximum concentration of VCM was 25.60 +/- 11.01 microg/g in the cornea 15 min after drug administration, and 5.18 +/- 2.60 microg/ml in the aqueous humour at 30 min. VCM in the aqueous humor could be detected (0.37 +/- 0.19 microg/ml) even after 240 min. VCM was not detected in the iris-ciliary body, vitreous, or serum at any point. The intraocular dynamics of 1% VCM ophthalmic ointment (TN-011) revealed that VCM reached the estimated effective concentrations in the aqueous humour as well as extraocular tissues of Bacillus subtilis-infected rabbit eyes. PMID:12673416

  18. TN-68 Spent Fuel Transport Cask Analytical Evaluation for Drop Events

    SciTech Connect

    Shah, M. J.; Klymyshyn, Nicholas A.; Adkins, Harold E.; Koeppel, Brian J.

    2007-03-30

    The U.S. Nuclear Regulatory Commission (NRC) is responsible for licensing commercial spent nuclear fuel transported in casks certified by NRC under the Code of Federal Regulations (10 CFR), Title 10, Part 71 [1]. Both the International Atomic Energy Agency regulations for transporting radioactive materials [2, paragraph 727], and 10 CFR 71.73 require casks to be evaluated for hypothetical accident conditions, which includes a 9-meter (m) (30-ft) drop-impact event onto a flat, essentially unyielding, horizontal surface, in the most damaging orientation. This paper examines the behavior of one of the NRC certified transportation casks, the TN-68 [3], for drop-impact events. The specific area examined is the behavior of the bolted connections in the cask body and the closure lid, which are significantly loaded during the hypothetical drop-impact event. Analytical work to evaluate the NRC-certified TN-68 spent fuel transport cask [3] for a 9-m (30-ft) drop-impact event on a flat, unyielding, horizontal surface, was performed using the ANSYS® [4] and LS DYNA™ [5] finite-element analysis codes. The models were sufficiently detailed, in the areas of bolt closure interfaces and containment boundaries, to evaluate the structural integrity of the bolted connections under 9-m (30-ft) free-drop hypothetical accident conditions, as specified in 10 CFR 71.73. Evaluation of the cask for puncture, caused by a free drop through a distance of 1-m (40-in.) onto a mild steel bar mounted on a flat, essentially unyielding, horizontal surface, required by 10 CFR 71.73, was not included in the current work, and will have to be addressed in the future. Based on the analyses performed to date, it is concluded that, even though brief separation of the flange and the lid surfaces may occur under some conditions, the seals would close at the end of the drop events, because the materials remain elastic during the duration of the event.

  19. Tn5-Induced and Spontaneous Switching of Sinorhizobium meliloti to Faster-Swarming Behavior†

    PubMed Central

    Wei, Xueming; Bauer, Wolfgang D.

    1999-01-01

    Tn5 mutants of Sinorhizobium meliloti RMB7201 which swarmed 1.5 to 2.5 times faster than the parental strain in semisolid agar, moist sand, and viscous liquid were identified. These faster-swarming (FS) mutants outgrew the wild type 30- to 40-fold within 2 days in mixed swarm colonies. The FS mutants survived and grew as well as or better than the wild type under all of the circumstances tested, except in a soil matrix subjected to air drying. Exopolysaccharide (EPS) synthesis was reduced in each of the FS mutants when they were grown on defined succinate-nitrate medium, but the extent of reduction was different for each. It appears that FS behavior likely results from a modest, general derepression of motility involving an increased proportion of motile and flagellated cells and an increased average number of flagella per cell and increased average flagellar length. Spontaneous FS variants of RMB7201 were obtained at a frequency of about 1 per 10,000 to 20,000 cells by either enrichment from the periphery of swarm colonies or screening of colonies for reduced EPS synthesis on succinate-nitrate plates. The spontaneous FS variants and Tn5 FS mutants were symbiotically effective and competitive in alfalfa nodulation. Reversion of FS variants to wild-type behavior was sporadic, indicating that reversion is affected by unidentified environmental factors. Based on phenotypic and molecular differences between individual FS variants and mutants, it appears that there may be multiple genetic configurations that result in FS behavior in RMB7201. The facile isolation of spontaneous FS variants of Escherichia coli and Pseudomonas aeruginosa indicates that switching to FS behavior may be fairly common among bacterial species. The substantial growth advantage of FS mutants and variants wherever nutrient gradients exist suggests that switching to FS forms may be an important behavioral adaptation in natural environments. PMID:10049888

  20. TnBP?Triton X-45 Treatment of Plasma for Transfusion Efficiently Inactivates Hepatitis C Virus

    PubMed Central

    Chou, Ming-Li; Burnouf, Thierry; Chang, Shun-Pang; Hung, Ting-Chun; Lin, Chun-Ching; Richardson, Christopher D.; Lin, Liang-Tzung

    2015-01-01

    Risk of transmission of hepatitis C virus (HCV) by clinical plasma remains high in countries with a high prevalence of hepatitis C, justifying the implementation of viral inactivation treatments. In this study, we assessed the extent of inactivation of HCV during minipool solvent/detergent (SD; 1% TnBP / 1% Triton X-45) treatment of human plasma. Luciferase-tagged infectious cell culture-derived HCV (HCVcc) particles were used to spike human plasma prior to treatment by SD at 31 ± 0.5°C for 30 min. Samples were taken before and after SD treatment and filtered on a Sep-Pak Plus C18 cartridge to remove the SD agents. Risk of cytotoxicity was assessed by XTT cell viability assay. Viral infectivity was analyzed based on the luciferase signals, 50% tissue culture infectious dose viral titer, and immunofluorescence staining for HCV NS5A protein. Total protein, cholesterol, and triglyceride contents were determined before and after SD treatment and C18 cartridge filtration. Binding analysis, using patient-derived HCV clinical isolates, was also examined to validate the efficacy of the inactivation by SD. SD treatment effectively inactivated HCVcc within 30 min, as demonstrated by the baseline level of reporter signals, total loss of viral infectivity, and absence of viral protein NS5A. SD specifically targeted HCV particles to render them inactive, with essentially no effect on plasma protein content and hemostatic function. More importantly, the efficacy of the SD inactivation method was confirmed against various genotypes of patient-derived HCV clinical isolates and against HCVcc infection of primary human hepatocytes. Therefore, treatment by 1% TnBP / 1% Triton X-45 at 31°C is highly efficient to inactivate HCV in plasma for transfusion, showing its capacity to enhance the safety of therapeutic plasma products. We propose that the methodology used here to study HCV infectivity can be valuable in the validation of viral inactivation and removal processes of human plasma-derived products. PMID:25658612

  1. Visible-near infrared reflectance spectroscopy for estimating soil total nitrogen contents in the Sanjiang Yuan Regions, China: a case study of Yushu County and Maduo County, Qinghai province

    NASA Astrophysics Data System (ADS)

    Gao, Xiaohong; Yang, Yang; Zhang, Wei; Jia, Wei; Li, Jianshan; Tian, Chengming; Zhang, Yanjiao; He, Linhua

    2014-11-01

    The objective of this paper was to evaluate the prediction ability of visible and near-infrared reflectance spectroscopy(VNIRP) to estimate soil total nitrogen concentration (TN) in the Sanjiang Yuan regions, Qinghai province, China by using partial least squares regression (PLSR) method. A total of 149 soil samples (0-30cm) were collected respectively from Yushu county and Maduo county in August 2012. The soil total nitrogen concentrations were measured using Vario EL ? elemental analyzer (Germany Elementar Inc), and the soil spectral reflectance of with spectral range 350-2500nm was measured using ASD FieldSpec 4 in the laboratory. PLSR model was used to relate the reflectance spectra and its five different pre-processing transformations including the first order derivative reflectance (FDR), the second order derivative reflectance(SDR), the logarithm of the inverse of the reflectance (Log (1/R)), the depth of the band (Band depth) and the first order derivative of the logarithm of reflectance (( Log R)') to the soil total nitrogen contents measured. The results showed that the combination of visible-near infrared reflectance spectroscopy and PLSR to estimate TN produced a good prediction ability with R2 < 0.76 and RPD < 2.01 in the Sanjiang Yuan regions. The best prediction ability was obtained by (Log R) ' with R2CV = 0.87, R2V =0.88 and RPD <2.67, followed by Log (1/R), Band Depth, SDR, FDR and Reflectance. Visible and near infrared spectroscopy provided a rapid and effective method for estimating soil TN from five different soil types in the Sanjiang Yuan Regions, Qinghai province.

  2. Geothermal development plan: Yuma County

    SciTech Connect

    White, D.H.; Goldstone, L.A.

    1982-08-01

    The Yuma County Area Development Plan evaluated the county-wide market potential for utilizing geothermal energy. The study identified four potential geothermal resource areas with temperatures less than 90/sup 0/C (194/sup 0/F), and in addition, two areas are inferred to contain geothermal resources with intermediate (90/sup 0/C to 150/sup 0/C, 194/sup 0/F to 300/sup 0/F) temperature potential. The resource areas are isolated, although one resource area is located near Yuma, Arizona. One resource site is inferred to contain a hot dry rock resource. Anticipated population growth in the county is expected to be 2 percent per year over the next 40 years. The primary employment sector is agriculture, though some light industry is located in the county. Water supplies are found to be adequate to support future growth without advese affect on agriculture. Six firms were found in Yuma County which may be able to utilize geothermal energy for process heat needs. In addition, several agricultural processors were found, concentrated in citrus processing and livestock raising. Geothermal energy utilization projections suggest that by the year 2000, geothermal energy may economically provide the energy equivalent of 53,000 barrels of oil per year to the industrial sector if developed privately. Geothermal utilization projections increase to 132,000 barrels of oil per year by 2000 if a municipal utility developed the resource.

  3. Archuleta County CO Lineaments

    SciTech Connect

    Zehner, Richard E.

    2012-01-01

    Citation Information: Originator: Earth Science &Observation Center (ESOC), CIRES, University of Colorado at Boulder Originator: Geothermal Development Associates, Reno, Nevada Publication Date: 2012 Title: Archuleta Lineaments Edition: First Publication Information: Publication Place: Reno Nevada Publisher: Geothermal Development Associates, Reno, Nevada Description: This layer traces apparent topographic and air-photo lineaments in the area around Pagosa springs in Archuleta County, Colorado. It was made in order to identify possible fault and fracture systems that might be conduits for geothermal fluids. Geothermal fluids commonly utilize fault and fractures in competent rocks as conduits for fluid flow. Geothermal exploration involves finding areas of high near-surface temperature gradients, along with a suitable “plumbing system” that can provide the necessary permeability. Geothermal power plants can sometimes be built where temperature and flow rates are high. To do this, georeferenced topographic maps and aerial photographs were utilized in an existing GIS, using ESRI ArcMap 10.0 software. The USA_Topo_Maps and World_Imagery map layers were chosen from the GIS Server at server.arcgisonline.com, using a UTM Zone 13 NAD27 projection. This line shapefile was then constructed over that which appeared to be through-going structural lineaments in both the aerial photographs and topographic layers, taking care to avoid manmade features such as roads, fence lines, and right-of-ways. These lineaments may be displaced somewhat from their actual location, due to such factors as shadow effects with low sun angles in the aerial photographs. Note: This shape file was constructed as an aid to geothermal exploration in preparation for a site visit for field checking. We make no claims as to the existence of the lineaments, their location, orientation, and nature. Spatial Domain: Extent: Top: 4132831.990103 m Left: 311979.997741 m Right: 331678.289280 m Bottom: 4116067.165795 m Contact Information: Contact Organization: Geothermal Development Associates, Reno, Nevada Contact Person: Richard “Rick” Zehner Address: 3740 Barron Way City: Reno State: NV Postal Code: 89511 Country: USA Contact Telephone: 775-737-7806 Spatial Reference Information: Coordinate System: Universal Transverse Mercator (UTM) WGS’1984 Zone 13N False Easting: 500000.00000000 False Northing: 0.00000000 Central Meridian: -105.00000000 Scale Factor: 0.99960000 Latitude of Origin: 0.00000000 Linear Unit: Meter Datum: World Geodetic System 1984 (WGS ’1984) Prime Meridian: Greenwich Angular Unit: Degree Digital Form: Format Name: Shape file

  4. Geothermal development plan: Maricopa county

    SciTech Connect

    White, D.H.

    1981-01-01

    Maricopa county is the area of Arizona receiving top priority since it contains over half of the state's population. The county is located entirely within the Basin and Range physiographic region in which geothermal resources are known to occur. Several approaches were taken to match potential users to geothermal resources. One approach involved matching some of the largest facilities in the county to nearby geothermal resources. Other approaches involved identifying industrial processes whose heat requirements are less than the average assessed geothermal reservoir temperature of 110/sup 0/C (230/sup 0/F). Since many of the industries are located on or near geothermal resources, geothermal energy potentially could be adapted to many industrial processes.

  5. J. DALTON YORK 110 Sheffield Ct., Cookeville, TN 38506 | 931-854-1068 | dyork@tntech.edu

    E-print Network

    Firoozabadi, Abbas

    J. DALTON YORK 110 Sheffield Ct., Cookeville, TN 38506 | 931-854-1068 | dyork@tntech.edu EDUCATION experimental membrane development -Continued support role for APPI project Proposal writing (based on interests for specific pore radius outcome in Co-impregnated MCM-41 zeolite catalyst: -Synthesized sample design set

  6. 2003 BELTWIDE COTTON CONFERENCES, NASHVILLE, TN -JANUARY 6-10 AGE STRUCTURE AND SEX RATIO OF LYGUS HESPERUS

    E-print Network

    Rosenheim, Jay A.

    1082 2003 BELTWIDE COTTON CONFERENCES, NASHVILLE, TN - JANUARY 6-10 AGE STRUCTURE AND SEX RATIO to investigate this phenomenon by asking the question: can differences in age structure and sex ratio among Lygus of age structures (adults versus nymphs) and sex ratios (males versus females) across ten different Lygus

  7. loredo@spacenet.tn.cornell.edu http://www.astro.cornell.edu/staff/loredo/bayes/ Outline Probability

    E-print Network

    Loredo, Thomas J.

    loredo@spacenet.tn.cornell.edu http://www.astro.cornell.edu/staff/loredo/bayes/ Outline #15; Parametric uncertainty Bernoulli, Laplace, Bayes, Gauss: : : p(x) describes how probability (plausibility) is distributed mathematically (large number theorems, Bayes's theorem). Frequentist: Probability describes \\randomness" Venn

  8. Testing and analyses of the TN24P PWR spent-fuel dry storage cask loaded with consolidated fuel

    Microsoft Academic Search

    M. A. McKinnon; T. E. Michener; M. F. Jensen; G. R. Rodman

    1989-01-01

    A performance test of a Transnuclear, Inc. TN-24P storage cask configured for pressurized water reactor (PWR) spent fuel was performed. The work was performed by the Pacific Northwest Laboratory (PNL) and Idaho National Engineering Laboratory (INEL) for the US Department of Energy Office of Civilian Radioactive Waste Management (OCRWM) and the Electric Power Research Institute. The performance test consisted of

  9. 52nd AIAA Aerospace Sciences Meeting AIAA-2014-0322 13 -17 Jan 2014, National Harbor, MD, Nashville, TN

    E-print Network

    Gordeyev, Stanislav

    to the turret geometry in-flight have been investigated using a pressure sensitive paint (PSP) [8, Nashville, TN 1 American Institute of Aeronautics and Astronautics Effect of Surface Unsteady Pressure Field , and Eric Jumper3 University of Notre Dame, Notre Dame, Indiana, 46545 Simultaneous point surface pressure

  10. PHYSICAL LINKAGE OF MULTIPLE DRUG RESISTANCE TRANSPOSON TN21 TO COLICIN PLASMIDS AND DISINFECTANT RESISTANCE GENE MERB IN AVIAN ESCHERICHIA COLI

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Recently, we have reported on the widespread distribution of multiple-drug resistance transposon, Tn21 among clinical, avian Escherichia coli isolates in North Georgia. Tn21 is a 21 kb transposon that contains an integron, a mobile genetic element that “captures” antibiotic resistance gene(s), strep...

  11. The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products.

    PubMed Central

    Sarnovsky, R J; May, E W; Craig, N L

    1996-01-01

    The bacterial transposon Tn7 translocates by a cut and paste mechanism: excision from the donor site results from double-strand breaks at each end of Tn7 and target insertion results from joining of the exposed 3' Tn7 tips to the target DNA. Through site-directed mutagenesis of the Tn7-encoded transposition proteins TnsA and TnsB, we demonstrate that the Tn7 transposase is a heteromeric complex of these proteins, each protein executing different DNA processing reactions. TnsA mediates DNA cleavage reactions at the 5' ends of Tn7, and TnsB mediates DNA breakage and joining reactions at the 3' ends of Tn7. Thus the double-strand breaks that underlie Tn7 excision result from a collaboration between two active sites, one in TnsA and one in TnsB; the same (or a closely related) active site in TnsB also mediates the subsequent joining of the 3' ends to the target. Both TnsA and TnsB appear to be members of the retroviral integrase superfamily: mutation of their putative DD(35)E motifs blocks catalytic activity. Recombinases of this class require a divalent metal cofactor that is thought to interact with these acidic residues. Through analysis of the metal ion specificity of a TnsA mutant containing a sulfur (cysteine) substitution, we provide evidence that a divalent metal actually interacts with these acidic amino acids. Images PMID:8947057

  12. Mobility and Generation of Mosaic Non-Autonomous Transposons by Tn3-Derived Inverted-Repeat Miniature Elements (TIMEs)

    PubMed Central

    Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

    2014-01-01

    Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons – Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element “captured” with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not only of transposons and plasmids, but also of other types of mobile genetic elements. PMID:25121765

  13. A rapid and simple method for the isolation of mutant variants regulating tissue-specific expression of the TnI gene through drug selection

    SciTech Connect

    Lee, Youngwon; Kim, Myoung Hee [Korea Research Institute of Bioscience and Biotechnology, Taejon (Korea, Republic of); Emerson, C.P. Jr. [Fox Chase Cancer Center, Philadelphia, PA (United States)

    1995-12-01

    TnINEO fusion gene was constructed by fusing 3.4-kbp of quail TnI genomic DNA sequences spanning the promoter to exon 5 and a neo gene in frame. A myoblast cell line was established after transfection of pTnINEO. Since this cell line was passaged several times, a high frequency of neomycin (G418) sensitivity conversion was detected. Two drug-resistant variants were analyzed through genomic Southern blot and S1 nuclease protection assay. One variant has a mutation(s) in the regulatory element that activated the dormant TnI promoter-enhancer in myoblast, and the other has shown the geonomic rearrangement. This result presented the possibility of isolating factor(s) that activate the muscle-specific TnI promoter simply by screening drug-resistant cells having appropriate mutations. 12 refs., 4 fig.

  14. Status Offender Project: County Data Reports--Buncombe County.

    ERIC Educational Resources Information Center

    Deimel, Betty; And Others

    The first of three reports on a North Carolina project to help counties plan community based status offender programs describes a statewide needs assessment to collect information about the needs of status offenders and youth at risk of entering the juvenile justice system. A questionnaire covered situational and behavioral problems experienced…

  15. Martin County Master Gardeners In cooperation with the Martin County Library System

    E-print Network

    Florida, University of

    Martin County Master Gardeners In cooperation with the Martin County Library System Present Recycling in Martin County Monday, September 23, 2013 - 6:00 PM Blake Library, 2351 SE Monterey Rd., Stuart at the curb in your blue container in Martin County? Or maybe your area is still using the blue and yellow

  16. 76 FR 35997 - Onions Grown in Certain Designated Counties in Idaho, and Malheur County, OR; Modification of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ...Grown in Certain Designated Counties in Idaho, and Malheur County, OR; Modification...regulation for onions handled under the Idaho-Eastern Oregon onion marketing order...onions grown in designated counties in Idaho, and Malheur County, Oregon, and...

  17. 76 FR 30152 - East Calloway County Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ...FRL-9310-4] East Calloway County Middle School Mercury Spill Site, Murray, Calloway County...the East Calloway County Middle School Mercury Spill Site located in Murray, Calloway...County [[Page 30153

  18. Robust rat pulmonary radioprotection by a lipophilic Mn N-alkylpyridylporphyrin, MnTnHex-2-PyP5+?

    PubMed Central

    Gauter-Fleckenstein, Benjamin; Reboucas, Julio S.; Fleckenstein, Katharina; Tovmasyan, Artak; Owzar, Kouros; Jiang, Chen; Batinic-Haberle, Ines; Vujaskovic, Zeljko

    2014-01-01

    With the goal to enhance the distribution of cationic Mn porphyrins within mitochondria, the lipophilic Mn(III)meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin, MnTnHex-2-PyP5+ has been synthesized and tested in several different model of diseases, where it shows remarkable efficacy at as low as 50 µg/kg single or multiple doses. Yet, in a rat lung radioprotection study, at higher 0.6–1 mg/kg doses, due to its high accumulation and micellar character, it became toxic. To avoid the toxicity, herein the pulmonary radioprotection of MnTnHex-2-PyP5+ was assessed at 50 µg/kg. Fischer rats were irradiated to their right hemithorax (28 Gy) and treated with 0.05 mg/kg/day of MnTnHex-2-PyP5+ for 2 weeks by subcutaneously-implanted osmotic pumps, starting at 2 h post-radiation. The body weights and breathing frequencies were followed for 10 weeks post-radiation, when the histopathology and immunohistochemistry were assessed. Impact of MnTnHex-2-PyP5+ on macrophage recruitment (ED-1), DNA oxidative damage (8-OHdG), TGF-?1, VEGF(A) and HIF-1? were measured. MnTnHex-2-PyP5+ significantly decreased radiation-induced lung histopathological (H&E staining) and functional damage (breathing frequencies), suppressed oxidative stress directly (8-OHdG), or indirectly, affecting TGF-?1, VEGF (A) and HIF-1? pathways. The magnitude of the therapeutic effects is similar to the effects demonstrated under same experimental conditions with 120-fold higher dose of ~5000-fold less lipophilic Mn(III)meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin, MnTE-2-PyP5+. PMID:24624330

  19. Robust rat pulmonary radioprotection by a lipophilic Mn N-alkylpyridylporphyrin, MnTnHex-2-PyP(5+).

    PubMed

    Gauter-Fleckenstein, Benjamin; Reboucas, Julio S; Fleckenstein, Katharina; Tovmasyan, Artak; Owzar, Kouros; Jiang, Chen; Batinic-Haberle, Ines; Vujaskovic, Zeljko

    2014-01-01

    With the goal to enhance the distribution of cationic Mn porphyrins within mitochondria, the lipophilic Mn(III)meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin, MnTnHex-2-PyP(5+) has been synthesized and tested in several different model of diseases, where it shows remarkable efficacy at as low as 50 µg/kg single or multiple doses. Yet, in a rat lung radioprotection study, at higher 0.6-1 mg/kg doses, due to its high accumulation and micellar character, it became toxic. To avoid the toxicity, herein the pulmonary radioprotection of MnTnHex-2-PyP(5+) was assessed at 50 µg/kg. Fischer rats were irradiated to their right hemithorax (28 Gy) and treated with 0.05 mg/kg/day of MnTnHex-2-PyP(5+) for 2 weeks by subcutaneously-implanted osmotic pumps, starting at 2 h post-radiation. The body weights and breathing frequencies were followed for 10 weeks post-radiation, when the histopathology and immunohistochemistry were assessed. Impact of MnTnHex-2-PyP(5+) on macrophage recruitment (ED-1), DNA oxidative damage (8-OHdG), TGF-?1, VEGF(A) and HIF-1? were measured. MnTnHex-2-PyP(5+) significantly decreased radiation-induced lung histopathological (H&E staining) and functional damage (breathing frequencies), suppressed oxidative stress directly (8-OHdG), or indirectly, affecting TGF-?1, VEGF (A) and HIF-1? pathways. The magnitude of the therapeutic effects is similar to the effects demonstrated under same experimental conditions with 120-fold higher dose of ~5000-fold less lipophilic Mn(III)meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin, MnTE-2-PyP(5+). PMID:24624330

  20. Public Health County of Santa Cruz

    E-print Network

    California at Santa Cruz, University of

    Public Health Division County of Santa Cruz HEALTH SERVICES AGENCY POST OFFICE BOX 962, 1080 Date: December 3, 2009 Phone: 831-454-4000 Email: hsadocpio@co.santa-cruz.ca.us County Health Department Announces Flu Vaccine Clinic Santa Cruz County Public Health will hold an additional walk

  1. Community Types and Mortality in Georgia Counties

    ERIC Educational Resources Information Center

    Young, Frank W.

    2012-01-01

    Using an "ecological regional analysis" methodology for defining types of communities and their associated mortality rates, this study of Georgia's 159 counties finds that the suburban and town centered counties have low mortality while the city-centered type predicts low mortality for the whites. The military-centered counties do not predict. The…

  2. Once Distressed, Jackson County Moves On.

    ERIC Educational Resources Information Center

    Baldwin, Fred D.

    1998-01-01

    In 1981, Jackson County, North Carolina, appeared on the first list of "distressed counties" in Appalachia. Since then, the rural county has made significant improvements by investing in its physical infrastructure (which also promotes tourism); fostering economic-development partnerships among governments, small businesses, and local colleges;…

  3. Tennessee Higher Education County Profiles, 2009

    ERIC Educational Resources Information Center

    Tennessee Higher Education Commission, 2009

    2009-01-01

    This report presents a localized perspective on Tennessee higher education for the year 2009, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; and (4) number of county residents participating in the state's primary need-based and merit-based financial aid…

  4. Low-Wage Counties Face Locational Disadvantages.

    ERIC Educational Resources Information Center

    Gibbs, Robert; Cromartie, John B.

    2000-01-01

    Small populations and remoteness are the most salient features of low-wage counties. These locational attributes coincide with fewer high-wage jobs, yet low wages within industries define low-wage counties more than industry composition. Although adults in low-wage counties have less education and labor force participation overall, the role played…

  5. Trouble Brewing in Orange County. Policy Brief

    ERIC Educational Resources Information Center

    Buck, Stuart

    2010-01-01

    Orange County will soon face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that Orange County faces a total $41.2 billion liability for retiree benefits that are underfunded--including $9.4 billion for the county pension system and an estimated…

  6. Genessee County Birth-to-Work

    E-print Network

    Genessee County Birth-to-Work September 2010 #12;Genesee County Birth to Work It and disparities #12;Genesee County Birth to Work Birth to Work is about all of us building and transforming our communities to effectively compete and prosper in the global knowledge economy #12;Genesee Birth to Work

  7. STATE OF TEXAS COUNTY OF ________________

    E-print Network

    Garbey, Marc

    AFFIDAVIT STATE OF TEXAS § § COUNTY OF ________________ § Before me, the undersigned Notary Public and correct. 2. I graduated or will graduate from a Texas high school or received my GED certificate in Texas. 3. I resided in Texas for three years leading up to graduation from high school or receiving my GED

  8. Fresno County Mock Trial Competition.

    ERIC Educational Resources Information Center

    Fresno City Unified School District, CA.

    THE FOLLOWING IS THE FULL TEXT OF THIS DOCUMENT: The Fresno County Office of Education and the Fresno Unified School District hosted the Mock Trial Competition. The state competition is sponsored by the Constitutional Rights Foundation, with cosponsorship from the California State Bar Association and the California Young Lawyer's Association. This…

  9. Teton County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau. Local Area Unemployment Statistics (LAUS). Non Seasonally Adjusted Unemployment Rate. (Retrieved, Pondera, Teton, Chouteau, and Cascade Chronic Disease Hospitalization Rates County Montana Stroke and Human Services (2010) Black, Asian/Pacific Islanders, Hispanic & NonHispanic Ancestry 6 US Census

  10. Prairie County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau. Local Area Unemployment Statistics (LAUS). Non Seasonally Adjusted Unemployment Rate. (Retrieved, Carter, and Treasure Chronic Disease Hospitalization Rates Region 1 Montana Stroke1 Per 100, Asian/Pacific Islanders, Hispanic & NonHispanic Ancestry 6 US Census Bureau (2010) 5 County Health

  11. Composting in Prince William County

    SciTech Connect

    Lyons, K.

    1995-10-01

    Hidden in a small industrial corner of Prince Williams County, in Northern Virginia, a composting facility, after its first flourishing year in business, has found itself part of a symbiotic triangle. Along with a landfill and a waste-to-energy (WTE) plant, the composting facility is one of three programs that make up a joint public-private venture and form a interjurisdictional solid waste/refuse exchange agreement. Faced with the prospects of having to close a landfill at the end of the year, a mandate on yard waste collection that was increasing collection tonnage, and no room for further landfill development, Fairfax County, with approximately 900,000 residents, needed help. In a turn-around situation, Prince William County--with approximately 240,000 residents, a low budget, and much space available for development--responded. Together the counties created a solid waste exchange. The basis for the program is a unity between the local governments on some solid waste issues and a composting facility. Composting, specifically yard waste composting, has been among the fastest-growing aspects of waste management. In 1990, the nation was composting 2% of its solid waste. By the end of 1995, according to the US EPA, between 4% and 7% of solid waste will be recovered through composting. The number of yard waste composting facilities operating has increased from 651 in 1988 to more than 3,000 in 1994.

  12. CHARLOTTE COUNTY Environmental & Extension Services

    E-print Network

    Watson, Craig A.

    outreach as part of this famous volunteer program. Speakers specializing in certain topics will present 30CHARLOTTE COUNTY Environmental & Extension Services 25550 Harbor View Road, Suite 3 Port Charlotte The Institute of Food and Agricultural Sciences is an Equal Employment Opportunity - Affirmative Action Employer

  13. Kinship Care in Walton County.

    ERIC Educational Resources Information Center

    Charon, Sara L.; Nackerud, Larry

    1996-01-01

    The quality of life of maltreated children placed with relatives was examined through interviews with nine families in Walton County (Georgia) who had taken in related children. Over half of the children had experienced some improvement in home life, school performance, and their physical and mental status. Kinship care families indicated needs…

  14. Flathead County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2 provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory.7% 64.3% Tobacco Use1 18.4% 19.3% Alcohol Use (binge + heavy drinking)1 21.1% 22.8% Obesity1

  15. Lincoln County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Census Bureau (2010) 5 County Health Ranking, Robert Wood Johnson Foundation (2012) Leading Causes are reviewed to determine if the child is uptodate with recommendations of the Advisory Council.2% 19.3% Alcohol Use (binge + heavy drinking)1 22.5% (Region 5) 22.8% Obesity1 20.8% 21

  16. Liberty County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2 provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory.8% Obesity1 26.3% 21.6% Overweight1 38.3% 37.8% No Leisure time for physical activity1 23.6% 20

  17. Powell County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2 provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory.8% Obesity1 18.8% 21.6% Overweight1 36.4% 37.8% No Leisure time for physical activity1 18.8% 20

  18. Custer County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Ranking, Robert Wood Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1 provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory.8% Obesity1 26.8% 21.6% Overweight1 37.8% 37.8% No Leisure time for physical activity1 27.9% 20

  19. Cascade County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory.3% 64.3% Tobacco Use1 19.0% 19.3% Alcohol Use (binge + heavy drinking)1 18.2% 22.8% Obesity1

  20. Yellowstone County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau (2010) 5 County Health Ranking, Robert Wood Johnson Foundation (2012) Leading Causes are reviewed to determine if the child is uptodate with recommendations of the Advisory Council.7% 19.3% Alcohol Use (binge + heavy drinking)1 16.6% 22.8% Obesity1 22.8% 21.6% Overweight1

  1. Dawson County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau (2010) 5 County Health Ranking, Robert Wood Johnson Foundation (2012) Leading Causes are reviewed to determine if the child is uptodate with recommendations of the Advisory Council Use1 20.5% 19.3% Alcohol Use (binge + heavy drinking)1 24.8% 22.8% Obesity1 26.8% 21

  2. Wibaux County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau (2010) 5 County Health Ranking, Robert Wood Johnson Foundation (2012) Leading Causes of Death are assessed each year. All children's records per provider assessed are reviewed to determine if the child + heavy drinking)1 24.8% 22.8% Obesity1 26.8% 21.6% Overweight1 37.8% 37.8% No Leisure time

  3. Missoula County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory.5% 64.3% Tobacco Use1 19.2% 19.3% Alcohol Use (binge + heavy drinking)1 24.1% 22.8% Obesity1

  4. Granite County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Ranking, Robert Wood Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1 provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory/A 64.3% Tobacco Use1 17.3% 19.3% Alcohol Use (binge + heavy drinking)1 24.0% 22.8% Obesity1

  5. Carter County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Ranking, Robert Wood Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1 provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory/A 64.3% Tobacco Use1 20.5% 19.3% Alcohol Use (binge + heavy drinking)1 24.8% 22.8% Obesity1

  6. Treasure County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Census Bureau (2010) 5 County Health Ranking, Robert Wood Johnson Foundation (2012) Leading Causes provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory/A 64.3% Tobacco Use1 20.5% 19.3% Alcohol Use (binge + heavy drinking)1 24.8% 22.8% Obesity1

  7. Fergus County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Ranking, Robert Wood Johnson Foundation (2012) Leading Causes of Death County1 Montana1,2 Nation2 1 provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory.8% Obesity1 24.2% 21.6% Overweight1 38.3% 37.8% No Leisure time for physical activity1 22.0% 20

  8. Phillips County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Census Bureau (2010) 5 County Health Ranking, Robert Wood Johnson Foundation (2012) Leading Causes are reviewed to determine if the child is uptodate with recommendations of the Advisory Council Use1 20.5% 19.3% Alcohol Use (binge + heavy drinking)1 24.8% 22.8% Obesity1 26.8% 21

  9. Tn/tau//sub E/ comparisons of tokamak reactors of different aspect ratios

    SciTech Connect

    Peng, Y.K.M.

    1989-01-01

    The ignition parameter Tn/tau//sub E/ of the International Tokamak Experimental Reactor (ITER) with an aspect ratio A = 2.6, a major radius R = 5.8 m, a plasma current I/sub p/ = 22 MA, and an external toroidal field of B/sub t0/ = 5 T at R; a High Field Experimental Reactor (HFER) with A = 6.0, R = 6.0 m, I/sub p/ = 8.2 MA, and B/sub t0/ = 12.4 T; and a Spherical Torus Experimental Reactor (STER) with A = 1.2, R = 1.5 m, I/sub p/ = 22 MA, and B/sub t0/ = 0.88 T are compared using a set of presently popular empirical or semi-empirical confinement scaling expressions. Since the physics basis for these scaling expressions are not determined, the results of comparison are best suited to helping identify issues critical to furthering our understanding of tokamak confinement. In the case where the plasma pressure is limited by the Troyon beta scaling using B/sub t0/, the scaling expressions with relatively strong A-dependence, favor the HFER, while those with relatively strong I/sub p/-dependence favor the STER or suggest comparable Tn/tau//sub E/. In the case where the plasma pressure is determined by a second stability /epsilon//beta//sub p/ limit (/epsilon/ = 1/A), the projections of the T-10 Goldston, Odajima-Shimamura, and Rebut-Lallia scaling expressions are also compared. There it is found that the above STER and HFER would suffice if /epsilon//beta//sub p/ = 0.5. These varied projections highlight the uncertain I/sub p/, A and R dependencies in empirical tokamak confinement scaling, which are likely to be resolved only by tests at very low and high A. The results also indicate that whether the plasma pressure is determined by a high /epsilon//beta//sub p/ limit is critical to the viability of the HFER and the STER compared to ITER, even if the above confinement uncertainties remain. 5 tabs.

  10. pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

    SciTech Connect

    Matsumoto, Yasuyuki; Zhang, Qing [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Akita, Kaoru; Nakada, Hiroshi [Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-ku, Kyoto 603-8555 (Japan)] [Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-ku, Kyoto 603-8555 (Japan); Hamamura, Kazunori; Tokuda, Noriyo [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Tsuchida, Akiko [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan) [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Noguchi Institute, 1-8-1 Kaga, Itabashi, Tokyo 173-0003 (Japan); Matsubara, Takeshi; Hori, Tomoko; Okajima, Tetsuya [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Furukawa, Keiko [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan) [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, 1200 Matsumoto-cho, Kasugai 487-8501 (Japan); Urano, Takeshi [Department of Biochemistry, Shimane University School of Medicine, Izumo 693-8501 (Japan)] [Department of Biochemistry, Shimane University School of Medicine, Izumo 693-8501 (Japan); Furukawa, Koichi, E-mail: koichi@med.nagoya-u.ac.jp [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer ppGalNAc-T13 was up-regulated in high metastatic sublines of Lewis lung cancer. Black-Right-Pointing-Pointer ppGalNAc-T13 expression enhanced cell invasion activity in low metastatic sublines. Black-Right-Pointing-Pointer Trimeric Tn antigen was induced in the transfectant cells of ppGalNAc-T13 cDNA. Black-Right-Pointing-Pointer A major protein carrying trimeric Tn structure was identified as Syndecan-1. Black-Right-Pointing-Pointer Silencing of ppGalNAc-T13 resulted in the reduction of invasion and of metastasis.. -- Abstract: In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. These sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated by subcutaneous injection of T13-KD clones showed lower coalescence to fascia and peritoneum, and significantly reduced lung metastasis than control clones. These data suggested that high expression of pp-GalNAc-T13 gene generated trimeric Tn antigen on Syndecan-1, leading to the enhanced metastasis.

  11. Clonal Expression of the Tn Antigen in Erythroid and Granulocyte Colonies and Its Application to Determination of the Clonality of the Human Megakaryocyte Colony Assay

    PubMed Central

    Vainchenker, William; Testa, Ugo; Deschamps, Jeanne Françoise; Henri, Annie; Titeux, Monique; Breton-Gorius, Janine; Rochant, Henri; Lee, Douglas; Cartron, Jean-Pierre

    1982-01-01

    To evaluate whether exposure of Tn determinants at the surface of human erythrocytes, platelets, and granulocytes could arise from a somatic mutation in a hemopoietic stem cell, burst-forming unit erythroid (BFU-E) colonies, colony-forming unit granulocyte-macrophage (CFU-GM), and colony-forming unit-eosinophil (CFU-Eo) were grown from a blood group O patient with a typical Tn syndrome displaying two distinct populations (Tn+ and Tn-) of platelets, granulocytes, and erythrocytes. A large number of colonies was observed. Individual colonies were studied with a fluorescent conjugate of Helix pomatia agglutinin (HPA). A sizeable fraction of each of the erythroid and granulocytic colonies appeared to consist exclusively of either HPA-positive or HPA-negative cells, thereby demonstrating the clonal origin of those exhibiting the Tn marker. Similar results were obtained from a second patient. These findings establish that the HPA labeling of Tn cells is an accurate marker permitting assessment of the clonality of the human megakaryocyte (MK) colony assay. For the study of MK cultures a double-staining procedure using the HPA lectin and a monoclonal antiplatelet antibody (J-15) was applied in situ to identify all MK constituting a colony. Our results, obtained in studies of 133 MK colonies, provide definitive evidence that the human MK colony assay is clonal because all MK colonies were exclusively composed of Tn+ and Tn- MK. Furthermore, the distribution of MK within a single colony was shown to be seminormal with a mean at 6 MK, isolated MK typically being absent in culture. Comparison of the proportion of mature Tn+ cells in blood with their respective Tn+ progenitors has also shown that no proliferative advantage occurs after the commitment; because Tn polyagglutinability is an acquired disorder, then the expansion of the Tn+ clone must occur either during the proliferative stage of the pluripotent stem cell or during the commitment itself. This study therefore affords evidence that a blood group antigen plays a role in the differentiation of a pluripotent stem cell. Images PMID:6175663

  12. Surface modifications in the platelets of a patient with alpha-N-acetyl-D-galactosamine residues, the Tn-syndrome.

    PubMed Central

    Nurden, A T; Dupuis, D; Pidard, D; Kieffer, N; Kunicki, T J; Cartron, J P

    1982-01-01

    The Tn-syndrome is an acquired disorder characterized by the polyagglutination of blood cells and the pathological exposure of alpha-N-acetyl-D-galactosamine residues (Tn-antigen) at the cell surface. We now report studies on the platelet of a patient (Ba.) of which 81% reacted positively with a fluorescein conjugate of Helix pomatia agglutinin (HPA). The surface proteins of Ba. platelets were labeled with 125I by the lactoperoxidase-catalyzed procedure; single and two-dimensional electrophoresis on sodium dodecyl sulfate (SDS)-polyacrylamide gels was followed by autoradiography that revealed normal 125I-labeling of the major membrane glycoproteins (GP) but that GP Ib had a faster than normal migration. the abnormal GP Ib of Ba. platelets was strongly labeled when platelet suspensions were treated sequentially with neuraminidase, galactose oxidase, and sodium [3H]borohydride. Unlike the GP Ib of normal human platelets, it was also strongly labeled when Ba. platelets were treated with galactose oxidase and sodium [3H]borohydride alone. Both the alloantigen, PlA1, and quinidine-dependent antibody receptor activity were normally expressed by Ba. platelets, which also bound a monoclonal antibody (AN51) to GP Ib. Analysis of Ba. platelets by crossed immunoelectrophoresis using a rabbit anti-human platelet antibody preparation revealed the presence of an immunoprecipitate in the GP Ib position that had an abnormal appearance and migration in the second dimension. An altered position of the precipitate given by Factor VIIIR:Ag was also noted. Incorporation of HPA into the agarose gel during the first dimension electrophoresis resulted in the specific precipitation of the abnormal GP Ib of Ba. platelets. Our studies show that circulating Tn-platelets contain GP Ib with a modified oligosaccharide chain structure responsible for the platelet expression of Tn-antigen activity. Images PMID:7174794

  13. Loss of catalase activity in Tn1545-induced mutants does not reduce growth of Listeria monocytogenes in vivo.

    PubMed Central

    Leblond-Francillard, M; Gaillard, J L; Berche, P

    1989-01-01

    Two catalase-negative mutants of Listeria monocytogenes were obtained by chromosomal insertions of the conjugative transposon Tn1545. The loss of catalase activity did not reduce the level of virulence of these mutants in mice. Indeed, both mutants were capable of growing in host tissues at the same rate as the parental catalase-positive strain. These results favor the view that catalase does not play a critical role in the resistance of L. monocytogenes to macrophage killing. PMID:2545629

  14. Precise Insertion of Antibiotic Resistance Determinants into Tn21Like Transposons: Nucleotide Sequence of the OXA-1 beta -lactamase Gene

    Microsoft Academic Search

    Marc Ouellette; Luc Bissonnette; Paul H. Roy

    1987-01-01

    Several plasmid-encoded beta -lactamase are on multiresistance transposable elements. The OXA-1 beta -lactamase gene is part of Tn2603, which is borne on the R plasmid RGN238. We report here the complete nucleotide sequence of the OXA-1 beta -lactamase gene and flanking sequences. The OXA-1 gene shows a >50% sequence divergence from the OXA-2 gene, yet there is significant functional similarity

  15. Monroe County Library System: Pathfinders

    NSDL National Science Digital Library

    Named after President James Monroe, Monroe County is part of upstate New York. The industrial metropolis of Rochester sits within its boundaries, and one of its key institutions is the Monroe County Library System. Over the past several years, the System has created this treasure trove of digital collections which dissect and organize the history of the area through historic maps, architectural guides, and an online history tour of places in Rochester that played a role in the abolition movement and the Civil War. First-time visitors might want to start out by looking over the "Raising the Flag: Patriotism in Rochester, 1892-1922" photo gallery and then move on the Susan B. Anthony Letters Collection. Library staff members have also created nine fine research guides for scholars and others, and they include a guide to Rochester immigrants and a house history guide.

  16. Origin and structural implications of upper Miocene rhyolites in Kingston Canyon, Piute County, Utah.

    USGS Publications Warehouse

    Rowley, P.D.; Steven, T.A.; Mehnert, H.H.

    1981-01-01

    Kingston Canyon is one of the deepest antecedent canyons in the High Plateaus subprovince of the Colorado Plateaus. Here the E Fork of the Sevier River flows westward transversely across the gently E tilted Sevier Plateau, which is developed on a basin-range fault block uplifted more than 1500m along the Sevier fault zone on the W. Upper Tertiary rhyolites, uncommon in SW Utah, occur both on the northern rim and in the bottom of Kingston Canyon. Those on the northern rim consist of lava flows and volcanic domes of the rhyolite of Forshea Mountain, dated by K/Ar methods at 7.6Ma old. Those in the bottom of Kingston Canyon, the rhyolite of Phonolite Hill, are especially well exposed and provide spectacular examples of a pyroclastic cone whose base is about at river level and a steep-sided volcanic dome emplaced into and through these deposits. The pyroclastic deposits, formerly 500 or more metres thick, consist of airfall, mudflow, and ash-flow(?) material of rhyolite and foreign lithic fragments especially olivine basalt. The dome consists of flow-banded, mostly devitrified rhyolite as much as 500m thick; it has been dated by K/Ar methods at 5.4Ma. In addition to the rhyolites, a dome and lava-flow complex, the rhyodacite of Dry Lake, occurs near the northern rim and is considered to postdate the rhyolite of Forshea Mountain and predate the rhyolite of Phonolite Hill. -from Authors

  17. Geologic map of the Fish Creek Reservoir 7.5' quadrangle, Blaine County, Idaho

    USGS Publications Warehouse

    Skipp, Betty; Brandt, Theodore R.

    2012-01-01

    The Fish Creek Reservoir quadrangle in south-central Idaho lies on the north-central margin of the Cenozoic Snake River Plain at the southern end of the Pioneer Mountains. Rocks exposed in the quadrangle range in age from Paleozoic through Cenozoic. Mesozoic rocks are absent. Though Triassic and Jurassic sedimentary rocks may have been deposited in this area, they have been removed by erosion following uplift and thrusting of the Late Cretaceous to early Tertiary Sevier orogeny. The Late Devonian to Early Mississippian Antler orogeny preceded the Sevier. Ordovician through Devonian rocks of western-derived shale and sandstone facies and eastern carbonate shelf facies are unconformably overlain respectively by Pennsylvanian-Permian Wood River and Mississippian Copper Basin Formations. These two sequences are exposed in structural windows juxtaposed by the Sevier-age Pioneer thrust fault. Interpretive cross-sections accompany the map. Volcanic rocks of the Eocene Challis Volcanic Group, Miocene Idavada Volcanics, and Pleistocene Snake River Group cover parts of the area that remains tectonically active.

  18. Carbon County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 2.8% 2.5% 2.6% Diabetes prevalence 6.9% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.3% 4.1% 6.0% All Sites Cancer 510.8 455.5 543.2 1 Community Health Data, MT Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 183.6 182

  19. Pondera County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 2.9% 2.5% 2.6% Diabetes prevalence 7.5% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.4% 4.1% 6.0% All Sites Cancer 461.9 455.5 543.2 1 Community Health Data, MT, Pondera, Teton, Chouteau, and Cascade Chronic Disease Hospitalization Rates County Montana Stroke

  20. Gallatin County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 1.0% 2.5% 2.6% Diabetes prevalence 2.7% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 2.4% 4.1% 6.0% All Sites Cancer 416.6 (Region 4) 455.5 543.2 1 Community Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 152.4 182

  1. Park County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    -related, poisoning, and suffocation. Chronic Disease Burden1 County Montana Nation3,4 Stroke prevalence 1.6% 2.5% 2.6% Diabetes prevalence 4.8% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.1% 4.1% 6.0% All Stroke Per 100,000 population 1 160.8 182.2 Diabetes Per 100,000 population 1 87.8 115.4 Myocardial

  2. Meagher County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 1.9% 2.5% 2.6% Diabetes prevalence 5.1% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 143.4 (Region 4) 182

  3. Fallon County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 2.9% 2.5% 2.6% Diabetes prevalence 7.7% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community Health Data, MT, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100

  4. Hill County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 2.6% 2.5% 2.6% Diabetes prevalence 6.7% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.0% 4.1% 6.0% All Sites Cancer 461.9 (Region 2) 455.5 543.2 1 Community County Montana Stroke1 Per 100,000 population 237.8 182.2 Diabetes1 Per 100,000 population 274

  5. Beaverhead County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 1.9% 2.5% 2.6% Diabetes prevalence 5.1% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 106.2 182

  6. Stillwater County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    transportation-related, poisoning, and suffocation. Chronic Disease Burden1 Region 3 Montana Nation3,4 Stroke prevalence 2.8% 2.5% 2.6% Diabetes prevalence 6.9% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart County Montana Stroke Per 100,000 population 1 186.1 182.2 Diabetes Per 100,000 population 1 46.1 115

  7. Madison County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 1.9% 2.5% 2.6% Diabetes prevalence 5.1% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 177.5 182.2 Diabetes1

  8. Mineral County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 2.5% 2.5% 2.6% Diabetes prevalence 5.6% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.0% 4.1% 6.0% All Sites Cancer 466.5 455.5 543.2 1 Community Health Data, MT, Missoula, and Ravalli Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100

  9. Glacier County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 3.2% 2.5% 2.6% Diabetes prevalence 9.6% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.5% 4.1% 6.0% All Sites Cancer 461.9 (Region 2) 455.5 543.2 1 Community County Montana Stroke1 Per 100,000 population 189.4 182.2 Diabetes1 Per 100,000 population 145

  10. Rosebud County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 2.2% 2.5% 2.6% Diabetes prevalence 8.7% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.6% 4.1% 6.0% All Sites Cancer 472.3 (Region 1) 455.5 543.2 1 Community River, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per

  11. Jefferson County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 1.9% 2.5% 2.6% Diabetes prevalence 5.1% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 123.7 182.2 Diabetes1

  12. Sanders County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ,4 Stroke prevalence 2.5% (Region 5) 2.5% 2.6% Diabetes prevalence 10.3% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.0% (Region 5) 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455 Rates County Montana Stroke1 Per 100,000 population 138.0 182.2 Diabetes1 Per 100,000 population

  13. Ravalli County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease provider assessed are reviewed to determine if the child is up-to-date with recommendations of the Advisory Use 1 18.3% 19.3% Alcohol Use (binge + heavy drinking) 1 18.7% 22.8% Obesity 1 19.7% 21.6% Overweight

  14. Chouteau County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease provider assessed are reviewed to determine if the child is up-to-date with recommendations of the Advisory Use 1 22.2% (Region 2) 19.3% Alcohol Use (binge + heavy drinking) 1 22.0% (Region 2) 22.8% Obesity 1

  15. Toole County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    ) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. CLRD* 1. Cancer 2 provider assessed are reviewed to determine if the child is uptodate with recommendations of the Advisory.8% Obesity1 26.3% 21.6% Overweight1 38.3% 37.8% No Leisure time for physical activity1 23.6% 20

  16. Daniels County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Causes of Death County1 Montana1,2 Nation2 1. Cancer 2. Heart Disease 3. CLRD* 1. Cancer 2. Heart Disease provider assessed are reviewed to determine if the child is up-to-date with recommendations of the Advisory Use 1 20.5% (Region 1) 19.3% Alcohol Use (binge + heavy drinking) 1 24.8% (Region 1) 22.8% Obesity 1

  17. Testing and analyses of the TN-24P PWR spent-fuel dry storage cask loaded with consolidated fuel

    SciTech Connect

    McKinnon, M A; Michener, T E; Jensen, M F; Rodman, G R

    1989-02-01

    A performance test of a Transnuclear, Inc. TN-24P storage cask configured for pressurized water reactor (PWR) spent fuel was performed. The work was performed by the Pacific Northwest Laboratory (PNL) and Idaho National Engineering Laboratory (INEL) for the US Department of Energy Office of Civilian Radioactive Waste Management (OCRWM) and the Electric Power Research Institute. The performance test consisted of loading the TN-24P cask with 24 canisters of consolidated PWR spent fuel from Virginia Power's Surry and Florida Power and Light's Turkey Point reactors. Cask surface and fuel canister guide tube temperatures were measured, as were cask surface gamma and neutron dose rates. Testing was performed with vacuum, nitrogen, and helium backfill environments in both vertical and horizontal cask orientations. Transnuclear, Inc., arranged to have a partially insulated run added to the end of the test to simulate impact limiters. Limited spent fuel integrity data were also obtained. From both heat transfer and shielding perspectives, the TN-24P cask with minor refinements can be effectively implemented at reactor sites and central storage facilities for safe storage of unconsolidated and consolidated spent fuel. 35 refs., 93 figs., 17 tabs.

  18. [Estimation of the TN and SOM contents in soil from GAN NAN navel orange plant area by NIR diffuse spectroscopy].

    PubMed

    Liu, Yan-De; Xiong, Song-Sheng; Chen, Dong-Bin

    2013-10-01

    The soil sampled from GAN NAN navel orange plant area was selected as research object, and the feasibility of analyzing the total nitrogen (TN) and soil organic matter (SOM) of soil was investigated by near infrared spectroscopy (NIR) techniques in the wavelength range of 4 000 - 7 500 cm(-1). Different pretreatment methods including multiplicative scatter correction (MSC), first derivative(1st D), second derivative (2nd D), Savitzkv-Golay (SG), standard normalized variate (SNV) and baseline were used. The partial least square regress (PLS) was built for the calibration models. The best TN model using SG pretreatment features the prediction correlation coefficients (r(c)) of 0.802, the root mean square error of calibration (RMSEC) of 2.754, the calibration correlation coefficients (r(p)) of 0.715, and the root mean square error of prediction (RMSEP) of 3.077 in the wave-length range of 4 000 - 7 500 cm(-1). The best SOM model using SNV pretreatment has r(c) of 0.848, RMSEC of 0.128, r(p) of 0.790, and RMSEP of 0.152. The results showed that the NIR diffuse reflectance can be used for quick estimate of the TN and SOM contents in soil with the wavelength range of 4 000 - 7 500 cm(-1). PMID:24409716

  19. Inversions and deletions generated by a mini-gamma delta (Tn1000) transposon.

    PubMed

    Wang, G; Xu, X; Chen, J M; Berg, D E; Berg, C M

    1994-03-01

    Intramolecular transposition by an engineered derivative of the transposon gamma delta (Tn1000) is described. This 1-kb element contains inverted repeats of the 40 bp of the delta end of gamma delta, bracketing a kan gene, but it contains no resolution site. Transposition was analyzed in two plasmids; one contained two contraselectable (conditional lethal) genes (thyA and sacB) adjacent to the mini-gamma delta element in a 13.0-kb pBR322/pUC-based two-component plasmid (a heterodimer), and the other contained a different contraselectable gene (strA [rpsL]) in a 13.2-kb three-component plasmid (a heterotrimer). Selection for loss of function of a single contraselectable gene yielded inversions and deletions. Each inversion plasmid was 1 kb larger than the parent plasmid: it had a second copy of mini-gamma delta inserted in the contraselected gene, with that copy plus the intervening segment inverted, and the 5-bp target site duplicated. Each deletion plasmid was smaller than the parent plasmid and had a deletion that extended from one transposon end into or through the contraselected gene for distances of up to 9.4 kb. The frequencies of deletions versus inversions ending in a single target gene were similar, although overall, deletions outnumbered inversions because deletions, but not inversions, into sites beyond the contraselected gene inactivate it. This work also demonstrates that thyA (which encodes thymidylate synthetase) is a useful contraselectable marker. PMID:8113172

  20. Mn-oxidizing Bacteria in Oak Ridge, TN and the Potential for Mercury Remediation

    NASA Astrophysics Data System (ADS)

    Wright, K. L.; McNeal, K. S.; Han, F. X.

    2012-12-01

    East Fork Poplar Creek (EFPC) in Oak Ridge, TN was highly contaminated with elemental mercury in the 1950 and 1960. The area is still experiencing the effects of mercury contamination, and researchers are searching for ways to remediate the EFPC. One possible mechanism for bioremediation is the use of biogenic Mn oxides to remove heavy metals from water systems. Six native Pseudomonas bacteria species were isolated from the EFPC in order to examine biogenic Mn oxides production and bioremediation of Oak Ridge slurries. To investigate the biochemical interactions of Pseudomonas and the native microbial communities with Hg, Mn, Fe, S, six different slurry treatment groups were compared using inductively coupled plasma-atomic emission spectrometry (ICP-AES) and cold vapor atomic absorption spectrometry (CVAAS). Oak Ridge slurries were autoclaved to inhibit microbial growth (group 1), autoclaved and amended with HgS (group 2), autoclaved and amended with Pseudomonas isolates and additional HgS (group 3), untreated slurry (group 4), normal slurry amended with HgS (group 5), and normal slurry amended with Pseudomonas isolates and additional HgS (group 6). The comparison of the autoclaved groups with the counterpart untreated and normal Oak Ridge slurries highlighted important microbial interactions. Also, the Pseudomonas isolates were grown separately in a MnSO4 media, and the individual bacteria were monitored for Mn-oxidization using ICP-AES and transmission electron microscopy (TEM). In the slurry sediments, the Pseudomonas isolates did produce Mn oxides which bound to mercury, and mercury bound to organic matter significantly decreased. However, after a significant decrease of dissolved mercury in the water, dissolved mercury was cycled back into the water system on day 10 of the study. Additionally, two individual native Oak Ridge Pseudomonas isolates demonstrated Mn-oxidization. Biogenic Mn oxides have the potential to decrease mercury cycling, however there is need for more in depth and long-term studies to confirm their sustained use as Hg bioremediators.

  1. Spatial variability assessment of soil nutrients in an intense agricultural area, a case study of Rugao County in Yangtze River Delta Region, China

    NASA Astrophysics Data System (ADS)

    Zhao, Yongcun; Xu, Xianghua; Darilek, Jeremy Landon; Huang, Biao; Sun, Weixia; Shi, Xuezheng

    2009-05-01

    Topsoil samples (0-20 cm) ( n = 237) were collected from Rugao County, China. Geostatistical variogram analysis, sequential Gaussian simulation (SGS), and principal component (PC) analysis were applied to assess spatial variability of soil nutrients, identify the possible areas of nutrient deficiency, and explore spatial scale of variability of soil nutrients in the county. High variability of soil nutrient such as soil organic matter (SOM), total nitrogen (TN), available P, K, Fe, Mn, Cu, Zn, and B concentrations were observed. Soil nutrient properties displayed significant differences in their spatial structures, with available Cu having strong spatial dependence, SOM and available P having weak spatial dependence, and other nutrient properties having moderate spatial dependence. The soil nutrient deficiency, defined here as measured nutrient concentrations which do not meet the advisory threshold values specific to the county for dominant crops, namely rice, wheat, and rape seeds, was observed in available K and Zn, and the deficient areas covered 38 and 11%, respectively. The first three PCs of the nine soil nutrient properties explained 62.40% of the total variance. TN and SOM with higher loadings on PC1 are closely related to soil texture derived from different parent materials. The PC2 combined intermediate response variables such as available Zn and P that are likely to be controlled by land use and soil pH. Available B has the highest loading on PC3 and its variability of concentrations may be primarily ascribed to localized anthropogenic influence. The amelioration of soil physical properties (i.e. soil texture) and soil pH may improve the availability of soil nutrients and the sustainability of the agricultural system of Rugao County.

  2. 32. Photocopy of original drawing in possession of the County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PROFILE OF HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY IN JOHNSON COUNTY, IOWA, 1897. (RIGHT 1/4 OF DRAWING) PLAN OF APPROACH FOR SUTLIFF'S FERRY BRIDGE OVER CEDAR RIVER, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

  3. Hudson County Community College Periodic Review Report. Executive Summary.

    ERIC Educational Resources Information Center

    Hudson County Community Coll., Jersey City, NJ.

    Hudson County Community College (HCCC) serves Hudson County, New Jersey. Although the county is the smallest in the state, its 610,000 residents make up one of the most diverse counties in New Jersey. Approximately 40% of residents are Hispanic, 12% are African-American, 10% are Asian, and 35% are White. The county is also home to a growing Middle…

  4. 75 FR 22100 - Nevada County and Placer County, CA, Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-27

    ...DEPARTMENT OF AGRICULTURE Forest Service Nevada County and Placer County, CA, Resource Advisory Committee AGENCY: Forest Service, USDA...benefiting National Forest System lands on the Humboldt-Toiyabe, Eldorado, Lake Tahoe Basin Management Unit and Tahoe National...

  5. Mobilization of Escherichia coli R1 silver-resistance plasmid pJT1 by Tn5Mob into Escherichia coli C600

    Microsoft Academic Search

    Mary-Ellen Starodub; Jack T. Trevors

    1990-01-01

    Summary Escherichia coli Rl is an Ag+-resistant strain that, as we have shown recently, harbours at least two large plasmids, pJT1 (83 kb) and pJT2 (77 kb). Tn5-Mob was introduced into theE. coli Rl host replicon via conjugation on membrane filters. The transfer functions of plasmid RP4-4 were provided in this process and Tn5-Mob clones mated withE. coli C600 yielded

  6. PCR analysis of insertion site specificity, transcription, and structural uniformity of the Lepidopteran transposable element IFP2 in the TN368 cell genome

    Microsoft Academic Search

    Teresa A. Elick; Christopher A. Bauser; Nicole M. Principe; Malcolm J. Fraser

    1996-01-01

    The IFP2 element is a unique Lepidopteran transposon that has been associated with spontaneous Baculovirus mutants isolated following passage of the virus in the TN-368 cell line. Independent genomic representatives of IFP2 from TN-368 cells show little sequence divergence, suggesting that IFP2 was recently introduced into this genome and is highly stable. IFP2 is inserted within AT-rich regions of the

  7. The conjugative transposon Tn 925 : enhancement of conjugal transfer by tetracycline in Enterococcus faecalis and mobilization of chromosomal genes in Bacillus subtilis and E. faecalis

    Microsoft Academic Search

    Olga R. Torres; Ruth Z. KormanZ; Stanley A. Zahler; Gary M. Dunny

    1991-01-01

    The effects of tetracycline on transfer of the conjugative, tetracycline-resistance transposon, Tn925, as well as the ability of the transposon to promote the transfer of chromosomal genes was examined in Enterococcus faecalis and Bacillus subtilis. To test for chromosomal transfer, multiply-marked strains of each organism, each carrying a single chromosomal copy of Tn925, were mated on filters with suitable recipient

  8. Mercuric Ion-Resistance Operons of Plasmid R100 and Transposon Tn501: The Beginning of the Operon Including the Regulatory Region and the First Two Structural Genes

    Microsoft Academic Search

    Tapan K. Misra; Nigel L. Brown; D. C. Fritzinger; R. D. Pridmore; W. M. Barnes; Linda Haberstroh; Simon Silver

    1984-01-01

    The mercuric ion-resistance operons of plasmid R100 (originally from Shigella) and transposon Tn501 (originally from a plasmid isolated in Pseudomonas) have been compared by DNA sequence analysis. The sequences for the first 1340 base pairs of Tn501 are given with the best alignment with the comparable 1319 base pairs of R100. The homology between the two sequences starts at base

  9. Fine structure of transposition genes of Tn 2603 and complementation of its tnpA and tnpR mutations by related transposons

    Microsoft Academic Search

    Michiyasu Tanaka; Tomoko Yamamoto; Tetsuo Sawai

    1983-01-01

    Summary The fine structure of the genes tnpA, tnpR and res of Tn2603 required for its own transposition, was determined. The order of the genes was tnpA-tnpR-res from the right end of the right hand side region in Tn2603, the tnpA and tnpR encoded gene products having molecular weights of 110,000 and 21,000, respectively. The 110,000 molecular weight polypeptides was

  10. Regional Specificity of Illegitimate Recombination by the Translocatable Amplicillin-Resistance Element Tn1 in the Genome of Phage P22

    PubMed Central

    Weinstock, George M.; Susskind, Miriam M.; Botstein, David

    1979-01-01

    Insertions of the translocatable ampicillin-resistance element Tn1 were selected in the genome of the temperate Salmonella phage P22 by growing the phage on hosts carrying the resistance plasmid RP4. Insertions of Tn1 into phage P22 are rare (10-10 per phage) and nonrandomly distributed in the P22 genome. They are found mainly in the vicinity of the P22 ant gene. Insertions within the ant gene are found at many (at least 15) genetically separable sites, are found equally frequently in both orientations and cause irreversible loss of gene function. Some insertions in ant appear to be associated with an adjecent deletion.—Prophage deletions were derived from P22::Tn1 phages by two methods. Low multiplicity transductants have nonrandomly distributed endpoints. One end is at or very near the site of the Tn1 insertion, and the other is in the vicinity of gene 12; however, there are many genetically distinguishable endpoints within gene 12. Prophage deletions selected as survivors of induction of a P22Ap mnt-ts lysogen have similarly nonrandom endpoints, with the Tn1-distal end frequently near the ant gene, as well as gene 12. Physical analysis of several prophage deletions suggests that the Tn1 is intact to the resolution of DNA electron microscopy and that the deletions begin at the end of the Tn1 insertion.—These results suggest that illegitimate recombination associated with Tn1 shows regional specificity (i.e., preference for some large areas of the P22 genome over other areas), but that within these regions is quite nonspecific. PMID:395016

  11. Organisation of the bph gene cluster of transposon Tn 4371, encoding enzymes for the degradation of biphenyl and 4-chlorobiphenyl compounds

    Microsoft Academic Search

    J. W. Lengeler C. Merlin; D. Springael; M. Mergeay; A. Toussaint

    1997-01-01

    Tn4371 is a 55 kb transposon which encodes enzymes for the degradation of biphenyl and 4-chlorobiphenyl compounds into benzoate\\u000a and 4-chlorobenzo-ate derivatives. We constructed a cosmid library of Tn4371 DNA. The bph genes involved in biphenyl\\/4-chlorobiphenyl degradation were found to be clustered in the middle of the transposon. Sequencing\\u000a revealed an organisation of the bph genes similar to that previously

  12. Specific nicking at the 3' ends of the terminal inverted repeat sequences in transposon Tn3 by transposase and an E. coli protein ACP

    Microsoft Academic Search

    Takafumi Maekawa; Katsuhiko Yanagihara; Eiichi Ohtsubo

    1996-01-01

    Background :T n 3, a bacterial transposon, carries tnpA gene encoding transposase which is essential for its transposition. The transposition of Tn3 has been reproduced in vitro in a cell extract containing transposase by using a plasmid carrying mini-Tn3 as the donor and another plasmid as the target. Transposase has the ability to bind to the 38-bp terminal inverted repeats

  13. Use of the Centaur TnI-Ultra Assay for Detection of Myocardial Infarction and Adverse Events in Patients Presenting With Symptoms Suggestive of Acute Coronary Syndrome

    Microsoft Academic Search

    Fred S. Apple; Stephen W. Smith; Lesly A. Pearce; Ranka Ler; MaryAnn M. Murakami

    BACKGROUND: We determined the diagnostic accuracy of the Advia Centaur TnI-Ultra assay for detecting myocardial infarction (MI) and assessing risk of ad- verseeventsinpatientspresentingwithischemicsymp- toms suggestive of acute coronary syndrome. METHODS: We measured cardiac troponin I (cTnI) on admission and 6-24 h after admission (follow-up) in plasma specimens from 371 consecutive patients. The end point was the first of cardiac event

  14. Lee County Government Division of Natural Resources

    E-print Network

    Demers, Nora Egan

    or local levels. Greenhouse gas regulation, DMS production affecting cloud formation. Biological control County that also rely directly good water quality... Fisheries, Real Estate, etc... #12;Caloosahatchee

  15. Transposon Tn5281 is the main distributor of the aminoglycoside modifying enzyme gene among isolates of Enterococcus faecalis in Tehran hospitals.

    PubMed

    Feizabadi, Mohammad Mehdi; Shokrzadeh, Leila; Sayady, Sara; Asadi, Soroor

    2008-10-01

    Infections with high levels of gentamicin-resistant (HLGR) isolates of Enterococcus faecalis are common in Tehran hospitals. Genes encoding such resistance are transmissible by conjugation at high frequency. The purpose of this study was to determine the existence of Tn5281 and its flanking aminoglycoside modifying enzyme gene aac(6')-aph(2") among 102 HLGR isolates of E. faecalis cultured from patients at three hospitals in Tehran, Iran. These isolates were detected by disks containing 120 microg of gentamicin and made 65% of all E. faecalis during the study period. DNA was extracted from HLGR isolates and subjected to PCR assays targeting aac(6')-aph(2") and conjugative transposon Tn5281. The amplified aac(6')-aph(2") gene was labeled with digoxigenin and probed with Tn5281 amplicons in dot blot hybridization assays. The aac(6')-aph(2") gene was detected in 91%-92% (n = 93) of the HLGR isolates. All isolates containing aac(6')-aph(2") were positive in long-PCR targeting Tn5281 and the probe hybridized with Tn5281 amplicons. The number of HLGR isolates of E. faecalis has increased considerably in Tehran hospitals. Tn5281 is the main cause of transmission of aac(6')-aph(2") to different isolates of E. faecalis in the hospitals studied. PMID:18923558

  16. Involvement of a chlorobenzoate-catabolic transposon, Tn5271, in community adaptation to chlorobiphenyl, chloroaniline, and 2,4-dichlorophenoxyacetic acid in a freshwater ecosystem

    SciTech Connect

    Fulthorpe, R.R.; Wyndham, R.C. (Carleton Univ., Ottawa, Ontario (Canada))

    1992-01-01

    A chlorobenzoate-catabolic transposon (Tn5271) was introduced on a conjugative plasmid (pBRC60) in the natural host, Alcaligenes sp. strain BR60, into lake water and sediment flowthrough microcosms. Experimental microcosms were exposed to micromolar levels of 3-chlorobenzoate, 4-chloroaniline, 2,4-dichlorophenoxyacetate, or 3-chlorobiphenyl. The populations of the host, BR60, and organisms carrying Tn5271 were monitored over a 100-day period by use of selective plate counts and the most-probable-number-DNA hybridization method. Populations of Tn5271-carrying bacteria were significantly higher in microcosms dosed with 3-chlorobenzoate, 4-chloroaniline, and 3-chlorobiphenyl than in the control microcosms, indicating that each of these chemicals exerts a selective force on this particular genotype in natural systems. The rates of 3-chlorobenzoate uptake and respiration correlated with Tn5271-carrying populations, as did the rates of 4-chloroaniline uptake and respiration. Plasmid transfer in the 3-chlorobenzoate- and 3-chlorobiphenyl-dosed microcosms resulted in the selection of three phenotypic clusters of chlorobenzoate degraders, only one of which was closely related to the original pBRC60 (Tn5271) donor, Alcaligenes sp. strain BR60. Bacteria dominating 3-chloroaniline-dosed microcosms carried IS1071, the class II insertion sequence that brackets Tn5271, on a plasmid unrelated to pBRC60. The importance of plasmid transfer and transposition during chemical adaptation is discussed.

  17. Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition

    SciTech Connect

    Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Matsuura, Masaaki [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University (Japan); Gomi, Yasuyuki [Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University (Japan); Takahashi, Michiaki [Research Foundation for Microbial Diseases of Osaka University (Japan); Yamanishi, Koichi [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.j [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Division of Clinical Virology, Kobe University Graduate School of Medicine (Japan)

    2010-06-20

    Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZ{alpha}-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cells as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.

  18. County Government in Georgia [And] Teacher's Manual for County Government in Georgia.

    ERIC Educational Resources Information Center

    Hepburn, Mary A.

    The student textbook and the teacher's manual focus on the services, organization, and funding of county government in Georgia. Designed to be used over a three to six week period, the textbook is arranged into six chapters. Chapter one discusses county government, its services, and its structure. Chapter two focuses on county officials and their…

  19. Martin County Master Gardeners In cooperation with the Martin County Library System

    E-print Network

    Watson, Craig A.

    Martin County Master Gardeners In cooperation with the Martin County Library System Present Plants of charge and sponsored by University of Florida/IFAS/Martin County Master Gardeners. Holiday plants will be available for purchase. Light refreshments will be served. http://martin.ifas.ufl.edu phone: 772

  20. 75 FR 79334 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-20

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. The Madera...

  1. 76 FR 29722 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-23

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  2. 76 FR 12316 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  3. 76 FR 6116 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  4. 75 FR 50987 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  5. 76 FR 66033 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...for expenditure of Payments to States Madera County Title II funds. DATES: The...

  6. 75 FR 31759 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...Send written comments to Julie Roberts, Madera County Resource Advisory Committee...

  7. 76 FR 19970 - Madera County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ...DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY...SUMMARY: The Madera County Resource Advisory Committee will...responsibilities for the remainder of the year. The Madera County Resource Advisory Committee...

  8. 7 CFR 7.25 - County executive director duties.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...2010-01-01 false County executive director duties. ...Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION... § 7.25 County executive director duties. ...pleasure of the county executive director. There shall...religion, color, sex, age, or...

  9. Silver City Grant County Airport (SVC) Pavement Condition and Analysis

    E-print Network

    Cal, Mark P.

    Silver City Grant County Airport (SVC) Pavement Condition and Analysis Submitted to: Jane M. Lucero ....................................1. Airport Conditions at Silver City Grant County Airport (SVC) 4 ..................................Figure 1. Geographic Location of Silver City Grant County Airport (SVC) 4 ......................Table 1

  10. 76 FR 61379 - Final Recovery Plan, Bexar County Karst Invertebrates

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-04

    ...Recovery Plan, Bexar County Karst Invertebrates AGENCY: Fish and Wildlife Service...plan, for the nine Bexar County Karst Invertebrates under the Endangered Species Act of...The following nine Bexar County karst invertebrates were listed as endangered species...

  11. 27 CFR 9.231 - Moon Mountain District Sonoma County.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...2014-04-01 2014-04-01 false Moon Mountain District Sonoma County. 9...American Viticultural Areas § 9.231 Moon Mountain District Sonoma County. (a...viticultural area described in this section is “Moon Mountain District Sonoma County”....

  12. 6. Photocopy of illustration on Map of Chester County Pennsylvania, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Photocopy of illustration on Map of Chester County Pennsylvania, by T.J. Kennedy, published by R.L. Barnes, Philadelphia, Pennsylvania, 1856 SOUTHWEST CORNER, 1856 - Bank of Chester County, 17 North High Street, West Chester, Chester County, PA

  13. 78 FR 20544 - Proposed Establishment of the Big Valley District-Lake County and Kelsey Bench-Lake County...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-05

    ...134] RIN 1513-AB99 Proposed Establishment of the Big Valley District-Lake County and Kelsey Bench-Lake County Viticultural Areas, and Modification of the Red Hills Lake County Viticultural Area AGENCY: Alcohol and Tobacco Tax and Trade...

  14. A Profile of Suwannee County, Florida.

    ERIC Educational Resources Information Center

    Beaulieu, Lionel J.

    Agriculture and the railroad were significant forces in the development of Suwannee County, Florida, formally created in 1858 but explored and settled beginning some 300 years earlier. Lumber and cotton caused an early 20th century boom in the county which soon saw the negative effects of both industries. The introduction of tobacco in the late…

  15. Wild Lake County Building Backyard Habitats

    E-print Network

    Florida, University of

    , and Coyotes, and Bears OH MY!! Humans are not the only animals who call Lake County home. Learn what to doWild Lake County Workshops Building Backyard Habitats Your backyard can be more than just a place species of wildlife. Alien Invaders Non-native plants and animals threaten the vitality of natural

  16. County Data Book 1995: Kentucky Kids Count.

    ERIC Educational Resources Information Center

    Kentucky Youth Advocates, Inc., Louisville.

    This data book presents findings of the Kids Count project on current conditions faced by Kentucky children age birth through 19. For each county, and for the state, comparisons are provided between the base years of 1980-1982 and the most recent years 1992-1994. Counties are ranked against each other and trend graphs are provided for the studied…

  17. [A Profile of Kershaw County, South Carolina].

    ERIC Educational Resources Information Center

    Lilley, Stephen C.; McLean, Edward L.

    Careful long-range planning and slow, deliberate growth have benefitted Kershaw County's 500,000 pleasantly varied acres near the state capitol. The county, famous for its equestrian activity, boasts prestige stables, riding clubs, and trails. In addition there are lakes and numerous parks. Leaders want to maintain this aesthetic appeal while…

  18. COUNTIES OF CALIFORNIA - WITH FARM STATISTICS

    EPA Science Inventory

    County farming statistics polygon coverage of California. The statistics were taken from the 1997 Census of Agriculture and added to a coverage extracted from TIGER county boundaries. A farm is any place from which $1,000 or more of agricultural products were sold, or normally w...

  19. Do's & Don'ts Manatee County

    E-print Network

    Jawitz, James W.

    Fertilizer Ordinance Do's & Don'ts Manatee County Things to look for in your lawn maintenance around water bodies and wetlands? For more information please contact us at: MANATEE COUNTY Division of Ag & Extension Service 941.722.4524 http://manatee.ifas.ufl.edu email manatee@ifas.ufl.edu ORDINANCE

  20. County Data Book 1997: Kentucky Kids Count.

    ERIC Educational Resources Information Center

    Kentucky Kids Count Consortium.

    This Kids Count data book examines trends in the well-being of Kentucky's children on a statewide and county basis. An introduction summarizes some of the trends for Kentucky's children in the 1990s. The bulk of the report presents statewide and county data grouped into five categories: (1) poverty rates and programs (persons in poverty; median…

  1. Crawford County Adult Education Center Program Evaluation

    ERIC Educational Resources Information Center

    Faubus, Deborah L.

    2011-01-01

    The Crawford County Adult Education Program Evaluation was implemented to gather data that would assist in increasing the number of enrolled students at CCAEC. The data were compiled from state, federal, local extant data, a Community Opinion Survey and focus group interviews. Of the 1,015 Crawford County Residents who were emailed the COS, 213…

  2. Tennessee County Higher Education Profiles, 2010

    ERIC Educational Resources Information Center

    Tennessee Higher Education Commission, 2010

    2010-01-01

    The "Tennessee County Higher Education Profiles 2010" provide basic information with respect to higher education for each county in Tennessee. The 2010 "Profiles" represent the fourth year for this report. In the demographic and enrollment sections, a few changes were made to better meet the needs of policymakers. This document explains those…

  3. Powder River County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau. Local Area Unemployment Statistics (LAUS). Non Seasonally Adjusted Unemployment Rate. (Retrieved, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100, Asian/Pacific Islanders, Hispanic & NonHispanic Ancestry 6 US Census Bureau (2010) 5 County Health

  4. Judith Basin County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau. Local Area Unemployment Statistics (LAUS). Non Seasonally Adjusted Unemployment Rate. (Retrieved Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 175.7 182, Asian/Pacific Islanders, Hispanic & NonHispanic Ancestry 6 US Census Bureau (2010) 5 County Health

  5. McCone County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    Bureau. Local Area Unemployment Statistics (LAUS). Non Seasonally Adjusted Unemployment Rate. (Retrieved, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100, Asian/Pacific Islanders, Hispanic & NonHispanic Ancestry 6 US Census Bureau (2010) 5 County Health

  6. Homelessness and Substance Use in Cook County

    E-print Network

    Illinois at Chicago, University of

    and Substance Abuse State of Illinois by Timothy P. Johnson and Mark E. Barrett Survey Research Laboratory University of Illinois September 1991 #12;#12;Homelessness & Substance Abuse in Cook County i CONTENTS Page ....................................................................................................49 #12;Homelessness & Substance Abuse in Cook County ii CONTENTS--Continued Page REFERENCES

  7. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...the proposed action. (c) Essential activities. For purposes of this section, the term “essential community development and...notify the county and HUD in writing by a date specified by HUD...notifying HUD and the county, in writing, of such election by a...

  8. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...the proposed action. (c) Essential activities. For purposes of this section, the term “essential community development and...notify the county and HUD in writing by a date specified by HUD...notifying HUD and the county, in writing, of such election by a...

  9. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...the proposed action. (c) Essential activities. For purposes of this section, the term “essential community development and...notify the county and HUD in writing by a date specified by HUD...notifying HUD and the county, in writing, of such election by a...

  10. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...the proposed action. (c) Essential activities. For purposes of this section, the term “essential community development and...notify the county and HUD in writing by a date specified by HUD...notifying HUD and the county, in writing, of such election by a...

  11. 24 CFR 570.307 - Urban counties.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...the proposed action. (c) Essential activities. For purposes of this section, the term “essential community development and...notify the county and HUD in writing by a date specified by HUD...notifying HUD and the county, in writing, of such election by a...

  12. Educational and Demographic Profile: Madera County

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Madera County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

  13. Groundwater management and protection Madison County, Alabama

    SciTech Connect

    French, J.H.; Strunk, J.W.

    1990-07-01

    Groundwater is extremely important to Madison County as it provides nearly three quarters of the county's drinking water. In recent years, Madison County has increasingly recognized the need to protect its groundwater resource. A supply of usable groundwater is one element of a high quality environment, which can help spur economic development and provide for the needs of a growing population. Without planning protection and understanding of possible consequences, however, economic development and population pressures can cause a gradual degradation of groundwater. In April 1987, the Tennessee Valley Authority (TVA) convened a local groundwater steering group in Madison County. At the first meeting the ground agreed upon these goals: (1) to seek incorporate groundwater protection into the planning and development process for Madison County, (2) to support efforts by Madison County to obtain authority to adopt zoning ordinances and subdivision regulations, and (3) to develop a groundwater management plan for the county. This report provides essential information needed in developing a plan and is based on the following assumptions: the citizens of Madison County value the environment in which they live and wish to protect it from pollution; continued economic development is necessary for a healthy local economy; and a healthy economy can be sustained and nurtured, without degradation of the groundwater resource, through countywide planning, education, and participation.

  14. Monroe County, New York 2013 Joint Community

    E-print Network

    Monroe County, New York 2013 Joint Community Service Plan 2014-2016 For Health Systems Serving for Community Health Finger Lakes Health System Agency #12;Monroe County Joint Community Service Plan 2014 community p. 4 IV. Public Participation p. 8 V. Selection of public health priorities p. 19 VI. Three Year

  15. Commodity Flow Study - Clark County, Nevada, USA

    SciTech Connect

    Conway, S.Ph.D. [Urban Environmental Research LLC, Las Vegas, NV (United States); Navis, I. [AICP Planning Manager, Clark County Nuclear Waste Division, Department of Comprehensive Planning, Las Vegas, NV (United States)

    2008-07-01

    The United States Department of Energy has designated Clark County, Nevada as an 'Affected Unit of Local Government' due to the potential for impacts by activities associated with the Yucca Mountain High Level Nuclear Waste Repository project. Urban Transit, LLC has led a project team of transportation including experts from the University of Nevada Las Vegas Transportation Research Center to conduct a hazardous materials community flow study along Clark County's rail and truck corridors. In addition, a critical infrastructure analysis has also been carried out in order to assess the potential impacts of transportation within Clark County of high level nuclear waste and spent nuclear fuel to a proposed repository 90 miles away in an adjacent county on the critical infrastructure in Clark County. These studies were designed to obtain information relating to the transportation, identification and routing of hazardous materials through Clark County. Coordinating with the United States Department of Energy, the U.S. Department of Agriculture, the U. S. Federal Highway Administration, the Nevada Department of Transportation, and various other stakeholders, these studies and future research will examine the risk factors along the entire transportation corridor within Clark County and provide a context for understanding the additional vulnerability associated with shipping spent fuel through Clark County. (authors)

  16. Geothermal development issues: Recommendations to Deschutes County

    SciTech Connect

    Gebhard, C.

    1982-07-01

    This report discusses processes and issues related to geothermal development. It is intended to inform planners and interested individuals in Deschutes County about geothermal energy, and advise County officials as to steps that can be taken in anticipation of resource development. (ACR)

  17. sarasotaCounty Number of volunteers 475

    E-print Network

    Jawitz, James W.

    , economic, or environmental benefits to community 67% Students and Alumni UF students from county 727 CALS Landscaping, Master Gardener, Commercial Horticulture, Local and Sustainable Agriculture, Family and Consumer Sciences, 4-H, Community Development Giving Recent donors residing in county 5,165 FY 2013 donors residing

  18. MonroeCounty Number of volunteers 116

    E-print Network

    Jawitz, James W.

    , economic, or environmental benefits to community 67% Students and Alumni UF students from county 155 CALS; Sustainable marine fisheries; Master Gardeners Giving Recent donors residing in county 957 FY 2013 donors in revenues. 21.2% contribution to gross regional product. Based on a 2011 UF study to the community. $1

  19. Big Horn County Secondary Data Analysis

    E-print Network

    Maxwell, Bruce D.

    transportation-related, poisoning, and suffocation. Chronic Disease Burden1 County Montana Nation3,4 Stroke prevalence 3.5% 2.5% 2.6% Diabetes prevalence 12.1% 6.2% 8.3% Acute Myocardial Infarction prevalence Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 230.7 182

  20. Educational and Demographic Profile: Tehama County

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Tehama County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

  1. Educational and Demographic Profile: Humboldt County.

    ERIC Educational Resources Information Center

    California Postsecondary Education Commission, 2004

    2004-01-01

    This profile uniquely presents a variety of educational and socioeconomic information for Humboldt County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

  2. COUNTY BOUNDARIES FOR THE MAIA STUDY REGION

    EPA Science Inventory

    This data set is a geographic information system (GIS) coverage of the county boundaries in the United States Environmental Protection Agency (USEPA) Mid-Atlantic Integrated Assessment (MAIA) Project region. The coverage was produced using the US Geological Survey county bound...

  3. A Profile of Anson County, North Carolina.

    ERIC Educational Resources Information Center

    Farr, M. Gaston; And Others

    Since 1950 Anson County, North Carolina, has had major contributions to economic development, a source of great concern to residents of the almost entirely rural area. The increased capacity of the Blewitt Falls Dam power output and the county-wide water filtration system (one of only a few in the United States today) are attractive to industry.…

  4. A Profile of Hardee County, Florida.

    ERIC Educational Resources Information Center

    Beaulieu, Lionel J.; Anderson, Deborah S.

    In 1977 leaders of Hardee County, Florida, listed relationships and attitudes of residents, rural atmosphere, environmental conditions, and economic potential among the county's strong points, and public service and facility improvements, developing economic potential, recreational and entertainment development, and planning and zoning as its most…

  5. Morgan County Scholarship Application Eligibility requirements

    E-print Network

    Stowell, Michael

    Morgan County Scholarship Application Eligibility requirements: This scholarship is for students who are from Morgan County, Colo., and are entering the University of Colorado at Boulder: * Telephone number: * Permanent address Street address 1: City: State: ZIP code: High school or secondary

  6. Analysis of County School Districts in Arkansas.

    ERIC Educational Resources Information Center

    Budd, Karol B.; Charlton, J.L.

    The 1948, Arkansas School District Reorganization Act was passed in an effort to reduce the 1589 small school districts to a smaller number. Those districts not consolidated would form county districts. As of the 1967-68 school year, 26 of these county districts remained. The purpose of this study was to provide information drawing attention to…

  7. Pennsylvania Dutch Country of Lancaster County

    NSDL National Science Digital Library

    The Pennsylvania Dutch Convention And Visitors Bureau, in cooperation with the attractions of the Amish Country of Lancaster County, Pennsylvania, now offer a complete virtual information center on the Pennsylvania Dutch Country of Lancaster County. Includes information on the Amish, quilts, covered bridges, and all the interesting things to see and do in the area.

  8. 76 FR 19030 - Lyon & Mineral County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ...DEPARTMENT OF AGRICULTURE Forest Service Lyon & Mineral County Resource Advisory Committee AGENCY: Forest Service...SUMMARY: The Lyon and Mineral County Resource Advisory Committee will meet in Smith...

  9. Rapid Transposon Liquid Enrichment Sequencing (TnLE-seq) for Gene Fitness Evaluation in Underdeveloped Bacterial Systems

    PubMed Central

    Fels, Samuel R.; Zane, Grant M.; Blake, Sean M.

    2013-01-01

    Whole-genome fitness analysis in microbes that uses saturating transposon mutagenesis combined with massively parallel sequencing (Tn-seq) is providing a measure of the contribution of each gene to a given growth condition. With this technique, gene fitness profiles and essential genes are discovered by simultaneous analyses of whether the absence of each gene product alters the growth kinetics of the bacterium. Here we modify the standard Tn-seq procedure to simplify and shorten the process by including delivery of the transposon through conjugation and liquid culture enrichment of the mutant pool, creating transposon liquid enrichment sequencing (TnLE-seq). To illustrate the success of these modifications and the robustness of the procedure, analyses of gene fitness of two cultures of the strictly anaerobic bacterium Desulfovibrio vulgaris Hildenborough were performed, with growth on lactate as the electron donor and sulfate as the electron acceptor. These data demonstrate reproducibility and provide a base condition for analysis of fitness changes in deletion mutants and in various growth conditions. The procedural modifications will facilitate the application of this powerful genetic analysis to microbes lacking a facile genetic system. Pilot studies produced 2.5 × 105 and 3.4 × 105 unique insertion mutants in the anaerobe Desulfovibrio vulgaris Hildenborough grown under typical laboratory conditions in rich medium. These analyses provided two similar high-resolution maps of gene fitness across the genome, and the method was also applied to growth in minimal medium. These results were also compared to the coverage obtained with a ca. 13,000-member cataloged transposon library constructed by sequencing transposon insertion sites in individual mutants. PMID:24077707

  10. Mutagenesis of the metJBLF gene cluster with transposon Tn5: localization of the metF transcriptional unit

    E-print Network

    Treat, Mary Love

    1986-01-01

    Gif881L1-38 was a gift of Dr. R. C. Greene, Duke University, Department of Biochemistry. Bacterio ha e and Plasmid Strains. Bacteriophage lambda c/857 amS7 and lambda dmet128 (cytR+, mefJ+B+L+F+) were supplied by Dr. James R. Johnson (52...: Robert C. Burghar (Chair of Committee) Billy G. Foster (Member) homas O. Baldwin (Co-Chair of Committee) Timothy C. Hall (Head of Department) December 1986 ABSTRACT Mutagenesis of the mefJBLF Gene Cluster with Transposon Tn5: Localization...

  11. Imperial County geothermal development annual meeting: summary

    SciTech Connect

    Not Available

    1983-01-01

    All phases of current geothermal development in Imperial County are discussed and future plans for development are reviewed. Topics covered include: Heber status update, Heber binary project, direct geothermal use for high-fructose corn sweetener production, update on county planning activities, Brawley and Salton Sea facility status, status of Imperial County projects, status of South Brawley Prospect 1983, Niland geothermal energy program, recent and pending changes in federal procedures/organizations, plant indicators of geothermal fluid on East Mesa, state lands activities in Imperial County, environmental interests in Imperial County, offshore exploration, strategic metals in geothermal fluids rebuilding of East Mesa Power Plant, direct use geothermal potential for Calipatria industrial Park, the Audubon Society case, status report of the Cerro Prieto geothermal field, East Brawley Prospect, and precision gravity survey at Heber and Cerro Prieto geothermal fields. (MHR)

  12. SCORE Science: Humboldt County Office of Education

    NSDL National Science Digital Library

    SCORE, a cooperative project of the California County Superintendent's Educational Services Association, has made K-12 teacher and student resources in four major subject areas available at sites in Humboldt County (Science), San Diego County (Language Arts), Kings County (Mathematics), and San Bernardino and Butte Counties (Social Studies). While each site has its own personality and delivery style, they all make an attempt to break out teacher and/or student resources by grade level. In some cases (Science, Social Studies) this is done through a search interface. In others (Language Arts, Mathematics) it is done via a browsable interface. It is this categorization, plus the fact that resources are selected, evaluated, and annotated by teachers, that is the both the power and unifying theme of the sites. Depending on the site, assessment and teacher development sections may also be available.

  13. Fall 2010 Davis County Tuesday Workshop

    NSDL National Science Digital Library

    Brooke Robertshaw

    2010-09-22

    Participant materials for those participating in the Tuesday Fall, 2010 Davis County IA Workshop . For viewing and printing convenience each resource is a pdf. For your convenience, there is a link to download the free Adobe Reader. Download Adobe Reader Workshop Materials Handouts Day 1 Workshop Handout and Materials Davis County Tuesday Day 2 Handouts Davis County Tuesday Day 3 Handouts Reflection Paper Prompts Fall 2010 Davis County Workshops First Reflection Prompts Fall 2010 Davis County Workshops Second Reflection Prompts Sample Instructional Architect Projects Solving One Step equations: Pre-Algebra Standard 3 Objective 2: Students will solve one step equations. The students will use addition and subtraction to solve the equations. Cells: Students will have the opportunity to explore the cell in a 3D fashion and learn more ...

  14. Environmental assessment: Deaf Smith County site, Texas

    SciTech Connect

    Not Available

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and the eight other potentially sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines. On the basis of these findings, the DOE is nominating the Deaf Smith County site as one of the five sites suitable for characterization. 591 refs., 147 figs., 173 tabs.

  15. Characterization of Tn1546 in Vancomycin-Resistant Enterococcus faecium Isolated from Canine Urinary Tract Infections: Evidence of Gene Exchange between Human and Animal Enterococci

    PubMed Central

    Simjee, S.; White, D. G.; McDermott, P. F.; Wagner, D. D.; Zervos, M. J.; Donabedian, S. M.; English, L. L.; Hayes, J. R.; Walker, R. D.

    2002-01-01

    Thirty-five enterococcal isolates were recovered from dogs diagnosed with urinary tract infections at the Michigan State University Veterinary Teaching Hospital over a 2-year period (1996 to 1998). Isolated species included Enterococcus faecium (n = 13), Enterococcus faecalis (n = 7), Enterococcus gallinarum (n = 11), and Enterococcus casseliflavus (n = 4). Antimicrobial susceptibility testing revealed several different resistance phenotypes, with the majority of the enterococcal isolates exhibiting resistance to three or more antibiotics. One E. faecium isolate, CVM1869, displayed high-level resistance to vancomycin (MIC > 32 ?g/ml) and gentamicin (MIC > 2,048 ?g/ml). Molecular analysis of this isolate revealed the presence of Tn1546 (vanA), responsible for high-level vancomycin resistance, and Tn5281 carrying aac6?-aph2", conferring high-level aminoglycoside resistance. Pulsed-field gel electrophoresis analysis revealed that CVM1869 was a canine E. faecium clone that had acquired Tn1546, perhaps from a human vancomycin-resistant E. faecium. Transposons Tn5281 and Tn1546 were located on two different conjugative plasmids. Sequence analysis revealed that in Tn1546, ORF1 had an 889-bp deletion and an IS1216V insertion at the 5? end and an IS1251 insertion between vanS and vanH. To date, this particular form of Tn1546 has only been described in human clinical vancomycin-resistant enterococcus isolates unique to the United States. Additionally, this is the first report of a vancomycin-resistant E. faecium isolated from a companion animal in the United States. PMID:12454168

  16. Analysis of the Shallow Groundwater Flow System at Fire Island National Seashore, Suffolk County, New York

    USGS Publications Warehouse

    Schubert, Christopher E.

    2010-01-01

    Fire Island National Seashore (FIIS) occupies 42 kilometers of the barrier island for which it is named that lies off the southern shore of Suffolk County, N.Y. Freshwater in the highly permeable, sandy aquifer underlying Fire Island is bounded laterally by marine surface waters and at depth by saline groundwater. Interspersed throughout FIIS are 17 pre-existing residential communities that in summer months greatly increase in population through the arrival of summer residents and vacationers; in addition, the National Park Service (NPS) has established several facilities on the island to accommodate visitors to FIIS. The 2.2 million people estimated by the NPS to visit Fire Island annually impact groundwater quality through the release of waste-derived contaminants, such as nutrients, pathogens, and organic compounds, into the environment. Waste-contaminated groundwater can move through the aquifer and threaten the ecological health of the adjacent back-barrier estuaries to which much of the groundwater ultimately discharges. In 2004, the U.S. Geological Survey (USGS), in cooperation with the NPS, began a 3-year investigation to (1) collect groundwater levels and water-quality (nutrient) samples, (2) develop a three-dimensional model of the shallow (water-table) aquifer system and adjacent marine surface waters, and (3) calculate nitrogen loads in simulated groundwater discharges from the aquifer to back-barrier estuaries and the ocean. The hydrogeology of the shallow aquifer system was characterized from the results of exploratory drilling, geophysical surveying, water-level monitoring, and water-quality sampling. The investigation focused on four areas-the communities of Kismet and Robbins Rest, the NPS Visitor Center at Watch Hill, and the undeveloped Otis Pike Fire Island High Dune Wilderness. Thirty-five observation wells were installed within FIIS to characterize subsurface hydrogeology and establish a water-table monitoring network in the four study areas. A variable-density model of the shallow aquifer system and adjacent marine surface waters was developed to simulate groundwater flow patterns and rates. Nitrogen loads from the shallow aquifer system were calculated from representative total nitrogen (TN) concentrations and simulated groundwater discharges to back-barrier estuaries and the ocean. The model simulates groundwater directions, velocities, and discharge rates under 2005 mean annual conditions. Groundwater budgets were developed for recharge areas of similar land use that contribute freshwater to back-barrier estuaries, the ocean, and subsea-discharge zones. Total freshwater discharge from the shallow aquifer system is about 43,500 cubic meters per day (m3/d) (79.8 percent) to back-barrier estuaries and about 10,200 m3/d (18.7 percent) to the ocean; about 836 m3/d (1.5 percent) may exit the system as subsea underflow. The total contribution of fresh groundwater to shoreline discharge zones amounts to about 53,700 m3/d (98.5 percent). The median age of freshwater discharged to back-barrier estuaries and the ocean was 3.4 years, and the 95th-percentile age was 20 years. The TN concentrations and loads under 2005 mean annual conditions for areas that contribute fresh groundwater to back-barrier estuaries and the ocean were calculated for the principal land uses on Fire Island. The overall TN load from the shallow aquifer system to shoreline discharge zones is about 16,200 kilograms per year (kg/yr) (82.2 percent) to back-barrier estuaries and about 3,500 kg/yr (17.8 percent) to the ocean. The overall TN load to marine surface waters amounts to about 19,700 kg/yr-roughly 6 percent of the annual TN load from shallow groundwater entering the South Shore Estuary Reserve (SSER) from the Suffolk County mainland, which is about 345,000 kg/yr. In contrast to the TN load from shallow groundwater for the SSER watershed, which annually yields about 353 kilograms per square kilometer (kg/km2), the overall TN loa

  17. Cloning and characterization of the tetracycline resistance determinant of and several promoters from within the conjugative transposon Tn919.

    PubMed Central

    Hill, C; Venema, G; Daly, C; Fitzgerald, G F

    1988-01-01

    Tn919 is a 15- to 16-kilobase (kb) tetracycline resistance conjugative transposon that was originally isolated from Streptococcus sanguis FC1. The tetracycline resistance determinant (tet) was found on a 4.2-kb HindII fragment by in vitro deletion analysis. This fragment was subcloned to a pWV01 origin capable of directing replication in Escherichia coli, Bacillus subtilis, and Streptococcus lactis, and expression was observed in all three genera. In all cases, expression was weaker when only the 4.2-kb cloned fragment rather than the full transposon was present. The resistance gene is of the streptococcal tetM class and codes for a protein of approximately 70 kilodaltons. The restriction map resembles that of the tetM gene of Tn1545 (P. Martin, P. Trieu-Cuot, and P. Courvalin, Nucleic Acids Res. 14:7047-7058, 1986), which codes for a protein of 72.5 kilodaltons. A number of transposon-derived promoter-bearing fragments were also cloned and sequenced. These closely resemble the consensus sequence of E. coli and B. subtilis promoters. Fusion experiments with a truncated lacZ gene indicate the possibility of an open reading frame for one of the promoters. Images PMID:2839111

  18. Costilla County Biodiesel Pilot Project

    SciTech Connect

    Doon, Ben; Quintana, Dan

    2011-08-25

    The Costilla County Biodiesel Pilot Project has demonstrated the compatibility of biodiesel technology and economics on a local scale. The project has been committed to making homegrown biodiesel a viable form of community economic development. The project has benefited by reducing risks by building the facility gradually and avoiding large initial outlays of money for facilities and technologies. A primary advantage of this type of community-scale biodiesel production is that it allows for a relatively independent, local solution to fuel production. Successfully using locally sourced feedstocks and putting the fuel into local use emphasizes the feasibility of different business models under the biodiesel tent and that there is more than just a one size fits all template for successful biodiesel production.

  19. Knox County Black History Archives

    NSDL National Science Digital Library

    2012-06-01

    Knox County, Ohio has had a significant black population for several hundred years, and historians and others have documented their activities in journal articles, books, and other printed materials. This remarkable digital collection from the Digital Resource Commons at Kenyon College provides access to photographs, documents, and other items that tell the story of this unique community. This collection was made possible, in part, by funding from the Ohio Humanities Council, along with support from the Rural Life Center. Currently the collection includes 200 items, which visitors can browse alphabetically or by performing a keyword search. Each item has a preview feature and visitors can click through to zoom in on each item. The handwritten history of Mt. Cavalry Baptist Church is particularly fascinating.

  20. Efficient role of BacTN635 on the safety properties, sensory attributes, and texture profile of raw minced meat beef and chicken breast.

    PubMed

    Smaoui, S; Elleuch, L; Ben Salah, R; Najah, S; Chakchouk-Mtibaa, A; Sellem, I; Besbes, S; Mellouli, L

    2014-01-01

    Bacteriocin BacTN635, produced by Lactobacillus plantarum sp. TN635, was purified and characterised in previous work. In this study we report the biotechnological application of this bacteriocin as a biopreservative during storage at 4°C of raw minced meat beef and chicken breast. Overall, the results obtained showed that the addition of the semi-purified BacTN635 at 500 or 1000 AU g(-1) in raw minced meat beef and chicken breast can delay the proliferation of spoilage microorganisms, suppress the growth of the pathogenic microorganism Listeria monocytogenes, improve sensory quality, texture attributes, and extend the shelf-life of these two meat products during refrigerated storage. BacTN635 at 1000 AU g(-1) could extend the shelf-life, and the meat showed good sensory characteristics. Therefore, treatment with semi-purified BacTN635 can be used as a safe method for preservation of raw minced meat beef and chicken breast. PMID:24320082

  1. Tri-county pilot study. [Texas

    NASA Technical Reports Server (NTRS)

    Reeves, C. A. (principal investigator); Austin, T. W.; Kerber, A. G.

    1976-01-01

    The author has identified the following significant results. An area inventory was performed for three southeast Texas counties (Montgomery, Walker, and San Jacinto) totaling 0.65 million hectares. The inventory was performed using a two level hierarchy. Level 1 was divided into forestland, rangeland, and other land. Forestland was separated into Level 2 categories: pine, hardwood, and mixed; rangeland was not separated further. Results consisted of area statistics for each county and for the entire study site for pine, hardwood, mixed, rangeland, and other land. Color coded county classification maps were produced for the May data set, and procedures were developed and tested.

  2. The Administrative Functions of the County Extension Director in Kansas.

    ERIC Educational Resources Information Center

    VanMeter, Earl Leroy

    The purpose of this study was to clarify the administrative functions of the Kansas county extension director through the process of role analysis. Respondents included four groups: all Kansas county extension directors, professional co-workers, county agricultural extension council executive board members in the selected counties, and selected…

  3. County Library Service to Rural Schools. Bulletin, 1930, No. 20

    ERIC Educational Resources Information Center

    Lathrop, Edith A.

    1930-01-01

    An efficient county library contemplates maximum service for money expended. Situated at the county seat or at some other centrally located place within the county, its book stock is carried to every resident of the county through a system branches, stations, and school deposits, and the services of an automobile and the parcel post. A trained…

  4. Lea County 001, an H5 chondrite, and Lea County 002, an ungrouped type 3 chondrite

    SciTech Connect

    Zolensky, M.E.; Score, R.; Clayton, R.N.; Mayeda, T.K.; Schutt, J.W. (NASA, Johnson Space Center, Houston, TX (USA) Lockheed Engineering and Sciences Co., Houston, TX (USA) Chicago Univ., IL (USA))

    1989-12-01

    A search of active deflation basins near Jal, Lea County, New Mexico resulted in the discovery of two meteorites, Lea County 001 and 002. Lea County 001 has mean olivine and low-Ca pyroxene compositions of Fa(19) and Fs(17), respectively. These and all other mineralogical and petrological data collected indicate a classification of H5 for this stone. Lea County 002 has mean olivine and low-Ca pyroxene compositions of Fa(2) and Fs(4), and is unequilibrated. Although it is mineralogically most similar to Kakangari and chondritic clasts within Cumberland Falls, the high modal amount of forsterite makes Lea County a unique type 3 chondrite. Oxygen isotope data for Lea County 002 fall on an 0-16-mixing line through those of the enstatite meteorites and IAB irons, a feature shared by Kakangari. 28 refs.

  5. The use of transposon insertion zdc-235::Tn10 (min 32) to clone and delete DNA from the terminus region of Escherichia coli.

    PubMed

    Henson, J M; Kuempel, P L

    1983-01-01

    Transposon zdc-235::Tn10 is inserted at min 32 on the genetic map of Escherichia coli, and we have used this transposon to clone 14 kb of DNA that flanks this insertion. The site of insertion of the transposon, and the restriction map of the cloned DNA, correspond well with the predictions of the Bouché restriction map for the terminus region (Bouché 1982). The zdc-235::Tn10 insertion, along with the zdd-230::Tn9 insertion, was used to obtain deletions of the region that has been cloned. Strains lacking a minimum of 14 kb, and more likely a minimum of 40 kb of DNA, showed no alteration of growth or cell morphology. PMID:6306397

  6. A TALE of Transposition: Tn3-Like Transposons Play a Major Role in the Spread of Pathogenicity Determinants of Xanthomonas citri and Other Xanthomonads

    PubMed Central

    Ferreira, Rafael Marini; de Oliveira, Amanda Carolina P.; Moreira, Leandro M.; Belasque, José; Gourbeyre, Edith; Siguier, Patricia; Ferro, Maria Inês T.; Ferro, Jesus A.

    2015-01-01

    ABSTRACT Members of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomonas citri plasmid pXAC64. These are flanked by short inverted repeats (IRs). The region was identified as a transposon, TnXax1, with typical Tn3 family features, including a transposase and two recombination genes. Two 14-bp palindromic sequences within a 193-bp potential resolution site occur between the recombination genes. Additional derivatives carrying different T3SEs and other passenger genes occur in different Xanthomonas species. The T3SEs include transcription activator-like effectors (TALEs). Certain TALEs are flanked by the same IRs as found in TnXax1 to form mobile insertion cassettes (MICs), suggesting that they may be transmitted horizontally. A significant number of MICs carrying other passenger genes (including a number of TALE genes) were also identified, flanked by the same TnXax1 IRs and delimited by 5-bp target site duplications. We conclude that a large fraction of T3SEs, including individual TALEs and potential pathogenicity determinants, have spread by transposition and that TnXax1, which exhibits all of the essential characteristics of a functional transposon, may be involved in driving MIC transposition. We also propose that TALE genes may diversify by fork slippage during the replicative Tn3 family transposition. These mechanisms may play a crucial role in the emergence of Xanthomonas pathogenicity. PMID:25691597

  7. A Role for Tn6029 in the Evolution of the Complex Antibiotic Resistance Gene Loci in Genomic Island 3 in Enteroaggregative Hemorrhagic Escherichia coli O104:H4

    PubMed Central

    Chowdhury, Piklu Roy; Charles, Ian G.; Djordjevic, Steven P.

    2015-01-01

    In enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104 the complex antibiotic resistance gene loci (CRL) found in the region of divergence 1 (RD1) within E. coli genomic island 3 (GI3) contains blaTEM-1, strAB, sul2, tet(A)A, and dfrA7 genes encoding resistance to ampicillin, streptomycin, sulfamethoxazole, tetracycline and trimethoprim respectively. The precise arrangement of antibiotic resistance genes and the role of mobile elements that drove the evolutionary events and created the CRL have not been investigated. We used a combination of bioinformatics and iterative BLASTn searches to determine the micro-evolutionary events that likely led to the formation of the CRL in GI3 using the closed genome sequences of EAHEC O104:H4 strains 2011C-3493 and 2009EL-2050 and high quality draft genomes of EAHEC E. coli O104:H4 isolates from sporadic cases not associated with the initial outbreak. Our analyses indicate that the CRL in GI3 evolved from a progenitor structure that contained an In2-derived class 1 integron in a Tn21/Tn1721 hybrid backbone. Within the hybrid backbone, a Tn6029-family transposon, identified here as Tn6029C abuts the sul1 gene in the 3´-Conserved Segment (-CS) of a class 1 integron generating a unique molecular signature that has only previously been observed in pASL01a, a small plasmid found in commensal E. coli in West Africa. From this common progenitor, independent IS26-mediated events created two novel transposons identified here as Tn6029D and Tn6222 in 2011C-3493 and 2009EL-2050 respectively. Analysis of RD1 within GI3 reveals IS26 has played a crucial role in the assembly of regions within the CRL. PMID:25675217

  8. Environmental assessment: Deaf Smith County site, Texas

    SciTech Connect

    Not Available

    1986-05-01

    In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of the nine potentially acceptable sites for mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines.

  9. DUST TRANSPORT IN MARICOPA COUNTY, ARIZONA

    EPA Science Inventory

    Numerical simulations have been carried out for ambient air supermicrometric particulate concentrations in Maricopa County, Arizona during late fall and winter periods of atmospheric stability. Results of model studies are in approximate agreement with limited field observational...

  10. Charging Up in King County, Washington

    ScienceCinema

    Constantine, Dow; Oliver, LeAnn; Inslee, Jay; Sahandy, Sheida; Posthuma, Ron; Morrison, David;

    2013-05-29

    King County, Washington is spearheading a regional effort to develop a network of electric vehicle charging stations. It is also improving its vehicle fleet and made significant improvements to a low-income senior housing development.

  11. A Seniors' Program for Beaver County

    ERIC Educational Resources Information Center

    Taylor, Donald F.

    1973-01-01

    Programs for seniors are needed, according to the assistant director of continuing education at the Community College of Beaver County (Pennsylvania); the question revolves around who will pay. (Editor)

  12. PENNSYLVANIA GEOGRAPHIC INFORMATION SYSTEM LIBRARY: COUNTY BOUNDARIES

    EPA Science Inventory

    Pennsylvania Geographic Information System (GIS) Library provides a data set of complete digitized county boundaries of the Commonwealth Of Pennsylvania at a scale of 1:24,000 from USGS topographic maps. The data format is ARC/INFO Export....

  13. Western Baldwin County, AL Grid Interconnection Project

    SciTech Connect

    Thomas DeBell

    2011-09-30

    The Objective of this Project was to provide an additional supply of electricity to the affected portions of Baldwin County, AL through the purchase, installation, and operation of certain substation equipment.

  14. Health Occupation Drilldowns for Milwaukee County

    E-print Network

    Saldin, Dilano

    Licensed Practical Nurses Dental Hygienists Occupational Therapists Occupational Therapy Assistants nurses, dental hygienists, occupational therapists, occupational therapy assistants, physical therapistsHealth Occupation Drilldowns for Milwaukee County Registered Nurses by Year License Granted

  15. County Employment Of West Virginia Higher

    E-print Network

    Mohaghegh, Shahab

    County Employment Of West Virginia Higher Education Graduates 2009 December 2010 Prepared I: Detailed Description Of Employment Data .........................................26 Appendix II. Employment Shares And Annualized Wages Of Graduates By Region Of Employment In 2009

  16. Aiken County Master Gardeners Vicky Bertagnolli

    E-print Network

    Duchowski, Andrew T.

    , Leptographium, the new pine design values, mid rotation thinnings and logging, non-native invasive species and herbicide controls, and the American Tree Farm System. Landowners from five or more South Carolina counties

  17. Environmental assessment, Deaf Smith County site, Texas

    SciTech Connect

    Not Available

    1986-05-01

    The Nuclear Waste Policy Act of 1982 (42 USC sections 10101-10226) requires the environmental assessment of a proposed site to include a statement of the basis for nominating a site as suitable for characterization. Volume 2 provides a detailed statement evaluating the site suitability of the Deaf Smith County Site under DOE siting guidelines, as well as a comparison of the Deaf Smith County Site to the other sites under consideration. The evaluation of the Deaf Smith County Site is based on the impacts associated with the reference repository design, but the evaluation will not change if based on the Mission Plan repository concept. The second part of this document compares the Deaf Smith County Site to Davis Canyon, Hanford, Richton Dome and Yucca Mountain. This comparison is required under DOE guidelines and is not intended to directly support subsequent recommendation of three sites for characterization as candidate sites. 259 refs., 29 figs., 66 refs. (MHB)

  18. 69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C.L. Wood, the county engineer. Updated through the mid-1930s to show new federal aid-state roads. Compares modern system with older county system. Original scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

  19. Hydrogeology of Cibola County, New Mexico

    USGS Publications Warehouse

    Baldwin, J.A.; Rankin, D.R.

    1995-01-01

    The hydrogeology of Cibola County, New Mexico, was evaluated to determine the occurrence, availability, and quality of ground-water resources. Rocks of Precambrian through Quaternary age are present in Cibola County. Most rocks are sedimentary in origin except for Precambrian igneous and metamorphic rocks exposed in the Zuni Uplift and Tertiary and Quaternary basalts in northern and central parts of the county. The most productive aquifers in the county include (youngest to oldest) Quaternary deposits, sandstones in the Mesaverde Group, the Dakota-Zuni-Bluff aquifer, the Westwater Canyon aquifer, the Todilto- Entrada aquifer, sandstone beds in the Chinle Formation, and the San Andres-Glorieta aquifer. Unconsolidated sand, silt, and gravel form a mantle ranging from a few inches to 150 to 200 feet over much of the bedrock in Cibola County. Well yields range from 5 to 1,110 gallons per minute. Dissolved-solids concentrations of ground water range from 200 to more than 5,200 milligrams per liter. Calcium, magnesium, bicarbonate, and sulfate are the predominant ions in ground water in alluvial material. The Mesaverde Group mainly occurs in three areas of the county. Well yields range from less than 1 to 12 gallons per minute. The predominant ions in water from wells in the Mesaverde Group are calcium, sodium, and bicarbonate. The transition from calcium-predominant to sodium-predominant water in the southwestern part of the county likely is a result of ion exchange. Wells completed in the Dakota-Zuni-Bluff aquifer yield from 1 to 30 gallons per minute. Dissolved-solids concentrations range from 220 to 2,000 milligrams per liter in water from 34 wells in the western part of the county. Predominant ions in the ground water include calcium, sodium, sulfate, and bicarbonate. Calcium predominates in areas where the aquifer is exposed at the surface or is overlain with alluvium. Sandstones in the Chinle Formation yield from 10 to 300 gallons per minute to wells in the Grants-Bluewater area. In the western part of the county, sodium and bicarbonate predominate in water from the Chinle Formation. In the eastern part of the county, water quality is more variable than elsewhere and the predominant constituents include calcium, sodium, sulfate, and chloride. Well yields from the San Andres-Glorieta aquifer in the Grants- Bluewater area are as much as 2,830 gallons per minute, whereas the maximum recorded pumping rate from the aquifer in other areas of the county is 88 gallons per minute. Dissolved-solids concentrations of ground-water range from about 130 to 4,200 milligrams per liter, and the water generally is a calcium bicarbonate sulfate type.

  20. Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements.

    PubMed

    Gutierrez, J A; Crowley, P J; Brown, D P; Hillman, J D; Youngman, P; Bleiweis, A S

    1996-07-01

    New vectors were constructed for efficient transposon Tn917-mediated mutagenesis of poorly transformable strains of Streptococcus mutans(pTV1-OK) and subsequent recovery of interrupted genes in Escherichia coli (pT21delta2TetM). In this report, we demonstrate the utility of Tn917 mutagenesis of a poorly transformable strain of S. mutans (JH1005) by showing (i) the conditional replication of pTV1-OK, a repA(Ts) derivative of the broad-host-range plasmid pWVO1 harboring Tn9l7, in JH1005 at the permissive temperature (30 degrees C) versus that at the nonpermissive temperature (45 degrees C); (ii) transposition frequencies similar to those reported for Bacillus subtilis (10(-5) to 10(-4)) with efficient plasmid curing in 90 to 97% of the erythromycin-resistant survivors following a temperature shift to 42 to 45 degrees C; and (iii) the apparent randomness of Tn917 insertion as determined by Southern hybridization analysis and the ability to isolate nutritional mutants, mutants in acid tolerance, and mutants in bacteriocin production, at frequencies ranging from 0.1 to 0.7%. Recovery of transposon-interrupted genes was achieved by two methods: (i) marker rescue in E. coli with the recovery vector pTV21delta2TetM, a tetracycline-resistant and ampicillin-sensitive Tn9l7-pBR322 hybrid, and (ii) "shotgun" cloning of genomic libraries of Tn917 mutants into pUC19. Sequence analyses revealed insertions at five different genetic loci in sequences displaying homologies to Clostridium spp.fhs (66% identity), E. coli dfp (43% identity), and B. subtilis ylxM-ffh (58% identity), icd (citC [69% identity]), and argD (61% identity). Insertions in icd and argD caused nutritional requirements; the one in ylxM-ffh caused acid sensitivity, while those in fhs and dfp caused both acid sensitivity and nutritional requirements. This paper describes the construction of pTV1-OK and demonstrates that it can be efficiently employed to deliver Tn917 into S. mutans for genetic analyses with some degree of randomness and that insertions in the chromosome can be easily recovered for subsequent characterization. This represents the first published report of successful Tn9l7 mutagenesis in the genus Streptococcus. PMID:8763945

  1. Digital geologic map of Beaver County, Oklahoma

    USGS Publications Warehouse

    Cederstrand, J.R.

    1997-01-01

    This data set consists of digital data and accompanying documentation for the surficial geology of Beaver County, Oklahoma. The original data are from the Hydrogeologic Map, sheet 1 of 3, included in the U.S. Geological Survey publication, Reconnaissance of the Water Resources of Beaver County, Oklahoma, Hydrologic Investigations Atlas HA-450, Morton and Goemaat, 1973. The geology was compiled by S.L. Schoff, 1953.

  2. SOLAR PANELS ON HUDSON COUNTY FACILITIES

    SciTech Connect

    BARRY, KEVIN

    2014-06-06

    This project involved the installation of an 83 kW grid-connected photovoltaic system tied into the energy management system of Hudson County's new 60,000 square foot Emergency Operations and Command Center and staff offices. Other renewable energy features of the building include a 15 kW wind turbine, geothermal heating and cooling, natural daylighting, natural ventilation, gray water plumbing system and a green roof. The County intends to seek Silver LEED certification for the facility.

  3. Edwards Aquifer Evaluation: Kinney County, Texas

    NASA Astrophysics Data System (ADS)

    Khorzad, Kaveh

    2003-10-01

    The Edwards Aquifer is one of the most studied and most prolific aquifers in the United States. The aquifer is a heavily fractured and faulted carbonate aquifer with transmissivities in excess of 100 ft2/s. The City of San Antonio relies upon the Edwards Aquifer as its sole source for water. Much work has been done on quantifying recharge to the aquifer and discharge from wells and acquiring aquifer characteristics from pumping tests, specific capacity tests, and geophysical logs. Although the aquifer has been well studied in Bexar County, much less is known about the Edwards Aquifer in Kinney County. This is partly due to the lower population within the county (approximately 3,500 people) relative to the eastern counties (Uvalde, Medina, Bexar, Comal, and Hays) and the great distance of Kinney County from high profile discharge areas such as the City of San Antonio and Comal and San Marcos Springs. Three key products resulted from this study: (1) exploratory well drilling and the largest aquifer test in the county that were conducted to evaluate the well yields within a 10,000 acre study area in which a drawdown of 2.5 ft approximately 1.2 miles away was observed while pumping at approximately 4,600 gpm; (2) a recharge estimate for the Edwards Aquifer within Kinney County of approximately 71,382 ac-ft/yr; and (3) locating the Brackettville Groundwater Divide from an evaluation of ground water flow direction and hydrograph analysis. These results help evaluate the complex hydraulics occurring within Kinney County and aid in development of ground water modeling that will be used in managing the Edwards Aquifer.

  4. The Water Cycle in Volusia County

    USGS Publications Warehouse

    German, Edward R.

    2009-01-01

    Earth's water is always in motion. The water cycle, also known as the hydrologic cycle, describes the continuous movement of water on, above, and below the Earth's surface. This fact sheet provides information about how much water moves into and out of Volusia County, and where it is stored. It also illustrates the seasonal variation in water quantity and movement using data from some of the hydrologic data collection sites in or near Volusia County, Florida.

  5. The flora of Oktibbeha County, Mississippi

    USGS Publications Warehouse

    Leidolf, A.; McDaniel, S.; Nuttle, T.

    2002-01-01

    We surveyed the flora of Oktibbeha County, Mississippi, U.S.A., from February 1994 to 1996. Occupying 118 square kilometers in east-central Mississippi, Oktibbeha County lies among 3 physiographic regions that include, from west to east, Interior Flatwoods, Pontotoc Ridge, and Black Prairie. Accordingly, the county harbors a diverse flora. Based on field work, as well as an extensive review of published literature and herbarium records at IBE and MISSA, we recorded a total of 1,148 taxa (1,125 species, 7 hybrids, 16 infraspecific taxa) belonging to 514 genera in 160 families, over 85% of all taxa documented were native. Compared to 3 other counties in east-central Mississippi, Oktibbeha County has the second largest recorded flora. The number of state-listed (endangered, threatened, or of special concern) taxa (67) documented in this survey far exceeds that reported from any other county in the region. Three introduced species, Ilex cornuta Lindl. & Paxton, Mahonia bealei (Fortune) Carrie??re, and Nandina domestica Thunb., are reported in a naturalized state for the first time from Mississippi. We also describe 16 different plant communities belonging to 5 broad habitat categories: bottomland forests, upland forests and prairies, aquatic habitats, seepage areas, and human-influenced habitats. A detailed description of the vegetation associated with each of these communities is provided.

  6. The Bridge of Mandolin County

    NASA Astrophysics Data System (ADS)

    Lantz, Juliette M.; Feindt, Jenny E.; Lewellyn, Eric P. B.; Walczak, Mary M.

    1999-12-01

    The Bridge of Mandolin County is a case designed to teach the general chemistry principles of molar mass, ions and aqueous reactions, solubility rules, and inorganic nomenclature. Through the instructor-facilitated class discussion, students consider the options before the Mandolin Town Council regarding deicing the newly constructed bridge connecting Mandolin with a large nearby city. The students must decipher contradictory claims made on behalf of sodium chloride, the traditional deicer, and calcium magnesium acetate, a new environmentally friendly deicer, to arrive at the most cost-effective and environmentally appropriate deicing product. As they work through the analysis they raise questions that can be addressed in a laboratory setting. Four optional role-playing experiments are included, which can be used by the students to gather information helpful to resolution of the case. The case is intended to be used over two class periods, with a laboratory period in between, though suggestions for other models are provided. Laboratory procedures include an EDTA titration for Ca2+ and Mg2+, a gravimetric analysis, a qualitative examination of ions and solubility, an introduction to freezing point depression and measurement, and an experimental design activity. This case can also successfully be used without alteration in non-majors chemistry or environmental chemistry courses, or upper-level analytical or environmental chemistry courses.

  7. Geothermal development plan: Maricopa County

    SciTech Connect

    White, D.H.; Goldstone, L.A.

    1982-08-01

    The Maricopa County Geothermal Development Plan evaluated the market potential for utilizing geothermal energy. The study identified six potential geothermal resource areas with temperatures less than 100{sup 0}C (212{sup 0}F) and in addition, four suspected intermediate temperature areas (90{sup 0} to 150{sup 0}C, 194{sup 0} to 300{sup 0}F). Geothermal resources are found to occur in and near the Phoenix metropolitan area where average population growth rates of two to three percent per year are expected over the next 40 years. Rapid growth in the manufacturing, trade and service sectors of the regional economy provides opportunities for the direct utilization of geothermal energy. A regional energy use analysis is included containing energy use and price projections. Water supplies are found to be adequate to support this growth, though agricultural water use is expected to diminish. The study also contains a detailed section matching geothermal resources to potential users. Two comparative analyses providing economic details for space heating projects are incorporated.

  8. IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China

    PubMed Central

    Chen, Zhenhong; Fang, Haihong; Wang, Li; Sun, Fengjun; Wang, Yong; Yin, Zhe; Yang, Huiying; Yang, Wenhui; Wang, Jie; Xia, Peiyuan; Zhou, Dongsheng; Liu, Changting

    2014-01-01

    Achromobacter xylosoxidans strain A22732 is isolated from a pneumonia patient in China and produces carbapenemases OXA-114e and IMP-1, which are encoded by chromosome and plasmid, respectively, and confer resistance to multiple ß-lactam antibiotics including carbapenems. The blaIMP-1 gene together with aacA7 and orfE is captured by a novel Tn402-like class 1 integron in a conjugative IncP-1ß plasmid. In addition to the intrinsic integron promoter PcW, there is still a blaIMP-1 gene cassette-specific promoter. This is the first report of carbapenemase-encoding IncP-1ß plasmid in clinical bacterial isolate. PMID:25428613

  9. Capability Description for NASA's F/A-18 TN 853 as a Testbed for the Integrated Resilient Aircraft Control Project

    NASA Technical Reports Server (NTRS)

    Hanson, Curt

    2009-01-01

    The NASA F/A-18 tail number (TN) 853 full-scale Integrated Resilient Aircraft Control (IRAC) testbed has been designed with a full array of capabilities in support of the Aviation Safety Program. Highlights of the system's capabilities include: 1) a quad-redundant research flight control system for safely interfacing controls experiments to the aircraft's control surfaces; 2) a dual-redundant airborne research test system for hosting multi-disciplinary state-of-the-art adaptive control experiments; 3) a robust reversionary configuration for recovery from unusual attitudes and configurations; 4) significant research instrumentation, particularly in the area of static loads; 5) extensive facilities for experiment simulation, data logging, real-time monitoring and post-flight analysis capabilities; and 6) significant growth capability in terms of interfaces and processing power.

  10. Annual Performance Evaluation of a Pair of Energy Efficient Houses (WC3 and WC4) in Oak Ridge, TN

    SciTech Connect

    Biswas, Kaushik [ORNL; Christian, Jeffrey E [ORNL; Gehl, Anthony C [ORNL; Jackson, Roderick K [ORNL; Boudreaux, Philip R [ORNL

    2012-04-01

    Beginning in 2008, two pairs of energy-saver houses were built at Wolf Creek in Oak Ridge, TN. These houses were designed to maximize energy efficiency using new ultra-high-efficiency components emerging from ORNL s Cooperative Research and Development Agreement (CRADA) partners and others. The first two houses contained 3713 square feet of conditioned area and were designated as WC1 and WC2; the second pair consisted of 2721 square feet conditioned area with crawlspace foundation and they re called WC3 and WC4. This report is focused on the annual energy performance of WC3 and WC4, and how they compare against a previously benchmarked maximum energy efficient house of a similar footprint. WC3 and WC4 are both about 55-60% more efficient than traditional new construction. Each house showcases a different envelope system: WC3 is built with advanced framing featured cellulose insulation partially mixed with phase change materials (PCM); and WC4 house has cladding composed of an exterior insulation and finish system (EIFS). The previously benchmarked house was one of three built at the Campbell Creek subdivision in Knoxville, TN. This house (CC3) was designed as a transformation of a builder house (CC1) with the most advanced energy-efficiency features, including solar electricity and hot water, which market conditions are likely to permit within the 2012 2015 period. The builder house itself was representative of a standard, IECC 2006 code-certified, all-electric house built by the builder to sell around 2005 2008.

  11. 76 FR 30080 - Revisions to the California State Implementation Plan, Placer County Air Pollution Control...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ...Implementation Plan, Placer County Air Pollution Control District and Ventura County Air Pollution Control District AGENCY: Environmental...revisions to the Placer County Air Pollution Control District (PCAPCD)...

  12. 76 FR 30025 - Revisions to the California State Implementation Plan, Placer County Air Pollution Control...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ...Implementation Plan, Placer County Air Pollution Control District and Ventura County Air Pollution Control District AGENCY: Environmental...revisions to the Placer County Air Pollution Control District (PCAPCD)...

  13. The Economic Impact of Johnson County Community College on Johnson County, 1993-94.

    ERIC Educational Resources Information Center

    Johnson County Community Coll., Overland Park, KS. Office of Institutional Research.

    In 1995, Johnson County Community College (JCCC), in Kansas, conducted a study to estimate the economic benefit contributed by the college to the Johnson County economy for 1993-94. Following a model developed by J. G. Ryan, direct economic impact was assessed by examining institutional expenditures, expenditures by college employees, and…

  14. The Economic Impact of Johnson County Community College on Johnson County, 1999-2000.

    ERIC Educational Resources Information Center

    Johnson County Community Coll., Overland Park, KS. Office of Institutional Research.

    This document is a report on the economic impact of Johnson County Community College (JCCC) (Kansas) on Johnson County and the surrounding community. The information from the economic impact study are used to support the community college's effectiveness, assessment, and accreditation efforts. Results indicate that: (1) the Community College…

  15. Beginnings of geothermal impact on county population and leadership, Imperial County, California

    SciTech Connect

    Pick, J.B.; Butler, E.W.

    1980-09-01

    A major geothermal energy development scenario is about to begin in Imperial County. Initial energy-socioeconomic interactions in the areas of population and county leadership structure are discussed. These include immigration of energy company workers, a sewage plant dispute, conflict over union affiliation of geothermal laborers, and a transmission corridor routing dispute.

  16. How Are Kentucky's Children Stacking Up? A County by County Analysis.

    ERIC Educational Resources Information Center

    Chandler, Betsy

    In a county by county analysis, this report assesses the quality of life for Kentucky's children. Researchers developed a child quotient (CQ) based on 18 indicators: per capita income, children in poverty, women receiving inadequate prenatal care, infant deaths, teens giving birth, substandard dwellings, children in foster care, per-pupil…

  17. 75 FR 42815 - Fulton County, LLC-Abandonment Exemption-in Fulton County, IN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ...AB 1064X] Fulton County, LLC--Abandonment Exemption--in Fulton County, IN...under 49 CFR 1152 subpart F--Exempt Abandonments to abandon its line of railroad between...any employee adversely affected by the abandonment shall be protected under Oregon...

  18. The California Child Care Portfolio, 1997: A Compilation of Data about Child Care, County by County.

    ERIC Educational Resources Information Center

    California Child Care Resource and Referral Network, San Francisco.

    This compendium provides standardized data on child care supply and requests for care in California. It provides county and statewide information based on responses from more than 38,000 child care providers and about 45,000 requests for child care, including: (1) key demographic statistics; (2) county population; (3) children in poverty; (4)…

  19. Agreement between Luzerne County Community College and Luzerne County Community College Association of Higher Education.

    ERIC Educational Resources Information Center

    Luzerne County Community Coll., Nanticoke, PA.

    This agreement between the Luzerne County Community College and the Luzerne County Community College Association of Higher Education covers the 1973-74 year. The agreement includes the collective bargaining agreement, definitions, recognition, fair practices, conference, maintenance of membership, check-off, grievance procedure, rights of college,…

  20. 76 FR 4254 - Irish Potatoes Grown in Certain Designated Counties in Idaho, and Malheur County, Oregon...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    ...AMS-FV-10-0109; FV11-945-1] Irish Potatoes Grown in Certain Designated Counties...conducted among eligible producers of Irish potatoes in certain designated counties...order regulating the handling of Irish potatoes grown in the production...