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Sample records for sevier county tn

  1. Seepage study of the Sevier River and the central Utah, McIntyre, and Leamington canals, Juab and Millard counties, Utah

    USGS Publications Warehouse

    Herbert, L.R.; Cruff, R.W.; Holmes, Walter F.

    1981-01-01

    (0.25 cubic meter per second) in the Sevier River and about 1.3 cubic feet per second (0.04 cubic meter per second) in the Leamington Canal. It also showed a net loss of about 7 cubic feet per second (0.20 cubic meter per second) in the Central Utah Canal and about 0.8 cubic foot per second (0.02 cubic meter per second) in the McIntyre Canal. The gains in the Sevier River and Leamington Canal probably come chiefly as return seepage of water lost from the Central Utah and McIntyre Canals.

  2. 77 FR 24200 - American Drum & Pallet, Memphis, Shelby County, TN; Notice of Settlement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-23

    ... AGENCY American Drum & Pallet, Memphis, Shelby County, TN; Notice of Settlement AGENCY: Environmental... settlement for reimbursement of past response costs concerning the American Drum and Pallet Superfund Site...-04- 2012-3770 or Site name American Drum & Pallet Superfund Site by one of the following methods:...

  3. Geological and petrophysical characterization of the Ferron Sandstone for 3-D simulation of a fluvial-deltaic reservoir. Deliverable 2.5.4, Ferron Sandstone lithologic strip logs, Emergy & Sevier Counties, Utah: Volume I

    SciTech Connect

    Allison, M.L.

    1995-12-08

    Strip logs for 491 wells were produced from a digital subsurface database of lithologic descriptions of the Ferron Sandstone Member of the Mancos Shale. This subsurface database covers wells from the parts of Emery and Sevier Counties in central Utah that occur between Ferron Creek on the north and Last Chance Creek on the south. The lithologic descriptions were imported into a logging software application designed for the display of stratigraphic data. Strip logs were produced at a scale of one inch equals 20 feet. The strip logs were created as part of a study by the Utah Geological Survey to develop a comprehensive, interdisciplinary, and qualitative characterization of a fluvial-deltaic reservoir using the Ferron Sandstone as a surface analogue. The study was funded by the U.S. Department of Energy (DOE) under the Geoscience/Engineering Reservoir Characterization Program.

  4. Modeling Interventions in the Owned Cat Population to Decrease Numbers, Knox County, TN.

    PubMed

    Lancaster, Evan P; Lenhart, Suzanne; Ojogbo, Ejebagom J; Rekant, Steven I; Scott, Janelle R; Weimer, Heidi; New, John C

    2016-01-01

    To find management strategies for controlling the owned cat population in Knox County, TN, the authors formulated a mathematical model using biological properties of such nonhuman animals and spay actions on certain age classes. They constructed this discrete-time model to predict the future owned cat population in this county and to evaluate intervention strategies to surgically sterilize some proportion of the population. Using the predicted population size and the number of surgeries for specific scenarios, they showed that focusing on specific age classes can be an effective feature in spay programs. PMID:27152694

  5. Extensional collapse along the Sevier Desert reflection, northern Sevier Desert basin, western United States

    NASA Astrophysics Data System (ADS)

    Coogan, James C.; Decelles, Peter G.

    1996-10-01

    Newly released and previously published seismic reflection data from the northern Sevier Desert basin provide a complete seismic transect between the tilted western margin of the basin and the eastern breakaway zone. When tied to well and surface age data, the transect delineates a continuum of extensional fault and basin fill geometries that developed between late Oligocene and Pleistocene time across the basin. A minimum of 18 km of top-to-the-west normal displacement is estimated across the Sevier Desert from only the most conspicuous growth geometries and offsets across listric normal faults that sole downward into the Sevier Desert reflection (SDR). The SDR clearly marks a normal fault zone beneath the entire basin, where stratal truncations are imaged for 50% of the 39 km length of the reflection east of the Cricket Mountains block. Restoration of extensional displacement along this entire 39 km fault length is necessary to reconstruct the pre-Oligocene configuration and erosion level of Sevier thrust sheets across the Sevier Desert area. The SDR normal fault zone underlies the former topographic crest of the Sevier orogenic belt, where it accommodated extensional collapse after cessation of regional contractile tectonism.

  6. Superfund record of decision amendment (EPA Region 4): Wrigley Charcoal Superfund Site, Hickman County, Wrigley, TN, February 2, 1995

    SciTech Connect

    1995-03-01

    This decision document presents the selected Interim Remedial Action (IRA) for the Wrigley Charcoal Site, in Wrigley, Hickman County, Tennessee. The U.S. EPA has modified a wide variety of items that require immediate response action for the first step of cleanup activities to be taken at the Wrigley Charcoal Site. The major goal of these cleanup activities is to address the most serious threats at the Wrigley Charcoal Site by removing contaminated media from the Primary Site flood plain, remediating wastes at the Storage Basin, and through limited access restrictions at the Primary Site and the Storage Basin. The cleanup activities as presented in this IRA Record of Decision (ROD) Amendment will achieve significant risk reduction and will prepare the Site for future remedial activities.

  7. 78 FR 28938 - Tennessee Disaster #TN-00075

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ... ADMINISTRATION Tennessee Disaster TN-00075 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 05/10... adversely affected by the disaster: Primary Counties: Stewart. Contiguous Counties: Tennessee: Benton,...

  8. 75 FR 55833 - Tennessee Disaster #TN-00042

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-14

    ... ADMINISTRATION Tennessee Disaster TN-00042 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 09/07... determined to be adversely affected by the disaster: Primary Counties: Putnam. Contiguous Counties:...

  9. 76 FR 18288 - Tennessee Disaster #TN-00048

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... ADMINISTRATION Tennessee Disaster TN-00048 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 03/23... determined to be adversely affected by the disaster: Primary Counties: Knox. Contiguous Counties:...

  10. Elkmont Vehicle Bridge, Construction PeelAway Great Smoky Mountains National ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Elkmont Vehicle Bridge, Construction Peel-Away - Great Smoky Mountains National Park Roads & Bridges, Elkmont Vehicle Bridge, Spanning Little River at Elkmont Campground, Gatlinburg, Sevier County, TN

  11. Dune field reactivation from blowouts: Sevier Desert, UT, USA

    NASA Astrophysics Data System (ADS)

    Barchyn, Thomas E.; Hugenholtz, Chris H.

    2013-12-01

    Dune field reactivation (a shift from vegetated to unvegetated state) has important economic, social, and environmental implications. In some settings reactivation is desired to preserve environmental values, but in arid regions reactivation is typically a form of land degradation. Little is known about reactivation due to a lack of published records, making modeling and prediction difficult. Here we detail dune reactivations from blowout expansion in the Sevier Desert, Utah, USA. We use historical aerial photographs and satellite imagery to track the transition from stable, vegetated dunes to actively migrating sediment in 3 locations. We outline a reactivation sequence: (i) disturbance breaches vegetation and exposes sediment, then (ii) creates a blowout with a deposition apron that (iii) advances downwind with a slipface or as a sand sheet. Most deposition aprons are not colonized by vegetation and are actively migrating. To explore causes we examine local sand flux, climate data, and stream flow. Based on available data the best explanation we can provide is that some combination of anthropogenic disturbance and climate may be responsible for the reactivations. Together, these examples provide a rare glimpse of dune field reactivation from blowouts, revealing the timescales, behaviour, and morphodynamics of devegetating dune fields.

  12. OBVERSE VIEW OF TN24911, LOOKING BACK FROM THE NORTHEAST ROOM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OBVERSE VIEW OF TN-249-11, LOOKING BACK FROM THE NORTHEAST ROOM TO THE STAIR HALL. THE DOOR TO THE NORTH (DINING) ROOM LIES AS LEFT - Hilderbrand House, 4571 Airways Boulevard, Memphis, Shelby County, TN

  13. Anatexis and metamorphism in tectonically thickened continental crust exemplified by the Sevier hinterland, western North America

    NASA Technical Reports Server (NTRS)

    Patino Douce, Alberto E.; Humphreys, Eugene D.; Johnston, A. Dana

    1990-01-01

    This paper presents a thermal and petrologic model of anatexis and metamorphism in regions of crustal thickening exemplified by the Sevier hinterland in western North America, and uses the model to examine the geological and physical processes leading to crustally derived magmatism. The results of numerical experiments show that anatexis was an inevitable end-product of Barrovian metamorphism in the thickened crust of the late Mesozoic Sevier orogenic belt and that the advection of heat across the lithosphere, in the form of mantle-derived mafic magmas, was not required for melting of metasedimentary rocks. It is suggested that, in the Sevier belt, as in other intracontinental orogenic belts, anatexis occurred in the midcrust and not at the base of the crust.

  14. New data on the late Pleistocene history of lake fluctuations in the Sevier Desert, Utah

    NASA Astrophysics Data System (ADS)

    Oviatt, C. G.; Spencer, J. Q.; Fan, Y.; Leggett, A.

    2010-12-01

    The chronology of lake-level changes during the late Pleistocene in the Lake Bonneville basin, Utah, is currently understood in a general way. Because the regressive phase of the lake occurred during a critical time in the history of the basin (climate was changing rapidly, humans were moving into suitable habitats that were being made available by the decline of the large lake), it is important to improve the dating of paleohydrologic and paleoclimatic events. We have initiated field and laboratory studies in a part of the Sevier Desert that was studied at a reconnaissance scale 25 to 30 years ago. Our work consists of describing critical stratigraphic sections in natural exposures along the Sevier River and in a core of lake and fluvial sediments, and in dating suitable materials from these sections using radiocarbon and optically stimulated luminescence (OSL). The Sevier basin was integrated with the rest of Lake Bonneville during the time when its highest shorelines were being formed, including during the long period of development of the Provo shoreline (~17.6-14.6 cal ka). The lake regressed rapidly from the Provo shoreline (~1465 m) to the altitude of the divide between the Sevier basin and the Great Salt Lake (GSL) basin (~1390 m), then split into two lakes. In the GSL basin the lake continued to decline as a closed-basin lake, and the lake in the shallow Sevier basin overflowed into it along a large river (the ancestral Sevier River). An extensive wetland, fed by runoff from the Sevier and Beaver Rivers, and by groundwater discharge, developed over much of the Sevier Desert floor during the early Holocene. We have measured new OSL ages that compare favorably with previously obtained radiocarbon ages for the same stratigraphic units. Part of our work is aimed at evaluating the hypothesis that rapid draw-downs in lake level would cause increased groundwater discharge to the lake, and therefore a potential radiocarbon reservoir effect that would have been

  15. Co-composting serves Tennessee county

    SciTech Connect

    Not Available

    1993-10-01

    This article describes how Sevier County, Tennessee cut its municipal waste stream by nearly 75 percent, far exceeding the current state waste diversion mandate of 25 percent. In the completely enclosed system, municipal waste is combined with sewage and septic sludge under conditions perfect to support rapid microbial activity. The result is humus, the organic component of soil.

  16. 15. Newfound Gap parking area with construction scar looking W. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Newfound Gap parking area with construction scar looking W. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

  17. 14. Newfound Gap Road, view from atop tunnel, Tennessee side. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Newfound Gap Road, view from atop tunnel, Tennessee side. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

  18. Corrigendum to "Dune field reactivation from blowouts: Sevier Desert, UT, USA" [Aeolian Res. 11 (2013) 75-84

    NASA Astrophysics Data System (ADS)

    Barchyn, Thomas E.; Hugenholtz, Chris H.

    2016-06-01

    This corrigendum corrects an error made in the flux calculations in 'Dune field reactivation from blowouts: Sevier Desert, UT, USA'. The corrected data differ only slightly from the original publication and do not affect the conclusions of the paper.

  19. Superfund Record of Decision (EPA Region 4): US DOE Oak Ridge Reservation, Operable Unit 6, Roane County, TN. (Fourth remedial action), September 1992. Interim report

    SciTech Connect

    Not Available

    1992-09-30

    The Oak Ridge Reservation (USDOE) (OU6) site is located within the K-25 plant, a former uranium enrichment facility in Oak Ridge, Roane County, Tennessee. Since the 1940's, the fabrication, decontamination, and maintenance processes associated with activities at the site, known as K-25, have produced hazardous and radioactive wastes. The ROD addresses an interim remedy to reduce the migration of contaminants and degradation of the environment caused by the SW31 discharge while the investigation of the K-1070 OU continues. The objective of the ROD, which addresses OU6, is to terminate the direct discharge of contaminants to surface water by intercepting and routing contaminated waters for treatment via an NPDES-permitted outfall prior to discharge to surface water. The primary contaminants of concern affecting the surface water are VOCs, including benzene, PCE, TCE, toluene, and xylenes; other organics, including PCBs and PAHs; and metals, including lead.

  20. Inventory of wetlands and agricultural land cover in the upper Sevier River Basin, Utah

    NASA Technical Reports Server (NTRS)

    Jaynes, R. A.; Clark, L. D., Jr.; Landgraf, K. F. (Principal Investigator)

    1981-01-01

    The use of color infrared aerial photography in the mapping of agricultural land use and wetlands in the Sevier River Basin of south central utah is described. The efficiency and cost effectiveness of utilizing LANDSAT multispectral scanner digital data to augment photographic interpretations are discussed. Transparent overlays for 27 quadrangles showing delineations of wetlands and agricultural land cover were produced. A table summarizing the acreage represented by each class on each quadrangle overlay is provided.

  1. Paired, facing monoclines in the Sanpete-Sevier Valley area, central Utah

    USGS Publications Warehouse

    Witkind, I.J.

    1992-01-01

    Several major monoclines that trend northward through the Sanpete-Sevier Valley area of central Utah are paired and face one another. This pairing of monoclines may have occurred when near-horizontal sedimentary and volcanic strata subsided into voids created as salt was removed from a salt diapir concealed beneath valley fill. Removal was mostly by dissolution or extrusion during Neogene time. The paired monoclines, thus, are viewed as collapse features rather than as normal synclinal folds. -from Author

  2. Superfund Record of Decision (EPA Region 4): Oak Ridge Reservation (USDOE), (Operable Unit 4), Roane County, Oak Ridge, TN. (Third remedial action), September 1991

    SciTech Connect

    Not Available

    1991-09-19

    The Oak Ridge Reservation (ORR) (USDOE) (Operable Unit 4) site is a former uranium isotope processing subsite in Oak Ridge, Roan County, Tennessee. The 1,700-acre K-25 site, which comprises Operable Unit 4 (OU4), is one of several hundred waste disposal sites or areas of contamination at the ORR site requiring Superfund remedial action. Land use in the area is predominantly residential. The storage yards contain 36,000 ninety-gallon drums of stabilized sludge, 29,000 ninety-gallon drums of raw sludge, and 16,000 gallons of raw sludge in tanks, contaminated with mixed radioactive and hazardous wastes. The Record of Decision (ROD) addresses the sludge stored at the storage yards as an interim action to prevent or mitigate releases to the environment. The selected remedial action includes eliminating free liquids in sludge through filter press, thermal drying, or similar methods; repacking dry sludge, followed by onsite storage of containers and processing liquids removed from the sludge through existing treatment facilities.

  3. Preliminary Feasibility Assessment of Geologic Carbon Sequestration Potential for TVA's John Sevier and Kingston Power Plants

    SciTech Connect

    Smith, Ellen D; Saulsbury, Bo

    2008-03-01

    This is a preliminary assessment of the potential for geologic carbon sequestration for the Tennessee Valley Authority's (TVA) John Sevier and Kingston power plants. The purpose of this assessment is to make a 'first cut' determination of whether there is sufficient potential for geologic carbon sequestration within 200 miles of the plants for TVA and Oak Ridge National Laboratory (ORNL) to proceed with a joint proposal for a larger project with a strong carbon management element. This assessment does not consider alternative technologies for carbon capture, but assumes the existence of a segregated CO{sub 2} stream suitable for sequestration.

  4. Ground-water hydrology and projected effects of ground-water withdrawals in the Sevier Desert, Utah

    USGS Publications Warehouse

    Holmes, Walter F.

    1984-01-01

    The principal ground-water reservoir in the Sevier Desert is the unconsolidated basin fill. The fill has been divided generally into aquifers and confining beds, although there are no clearcut boundaries between these units--the primary aquifers are the shallow and deep artesian aquifers. Recharge to the ground-water reservoir is by infiltration of precipitation; seepage from streams, canals, reservoirs, and unconsumed irrigation water; and subsurface inflow from consolidated rocks in mountain areas and from adjoining areas. Discharge is by wells, springs, seepage to the Sevier River, evapotranspiration, and subsurface outflow to adjoining areas.

  5. Late Cretaceous extension in the hinterland of the Sevier thrust belt, northwestern Utah and southern Idaho

    SciTech Connect

    Wells, M.L.; Allmendinger, R.W. ); Dallmeyer, R.D. )

    1990-10-01

    Cover rocks of the Raft River metamorphic core complex, located in the Sevier belt hinterland, preserve a structural and metamorphic history that predates the middle Tertiary extension of the region. In the eastern Raft River Mountains, Cambrian( )-Permian rocks form two allochthons that occur in the hanging wall of the mid-Miocene Raft River detachment fault. Dramatically attenuated, metamorphosed Cambrian( )-Pennsylvanian strata of the lower allochthon yield Late Cretaceous muscovite {sup 40}Ar/{sup 39}Ar plateau ages (ca. 82-90 Ma) that are interpreted to date cooling during final stages of layer parallel extension. Devonian-Permian rocks exposed in the Black Pine Mountains underwent east-west extension ({approximately} 160%) associated with syntectonic growth of white mica along cleavage. {sup 40}Ar/{sup 39}Ar whole-rock spectra of associated slates also indicate a Late Cretaceous metamorphism. Together, field and {sup 40}Ar/{sup 39}Ar results suggest that Late Cretaceous extension occurred in the Sevier belt hinterland at the same time as shortening in the eastern foreland and at depth in the hinterland. Sufficient topography must have been present to drive upper-crustal extension in the eastern hinterland.

  6. Cretaceous crustal structure and metamorphism in the hinterland of the Sevier thrust belt, western US Cordillera

    SciTech Connect

    Miller, E.L.; Gans, P.B. )

    1989-01-01

    Combined structural and geochronologic studies to the west of the Sevier belt at lat 39{degree}-40{degree}N indicate that peak metamorphism of the upper 15 km of crust occurred during the Late Cretaceous (70-90 Ma), overlapping in time with the intrusion of muscovite-bearing granites and pegmatites. Metamorphism was accompanied by penetrative deformation (top-to-the-east layer-parallel shear); both were localized at the currently exposed structural levels, resulting in impressive vertical and lateral strain gradients and T{sub max} gradients of > 50 C/km. Inception of thrust faulting in the Sevier belt may have predated peak metamorphism and intrusion of granites in the hinterland by 10-15 m.y., but it continued during and ended simultaneously with the end of metamorphism at about 70 Ma. The rise of muscovite-bearing granites and hot fluids, generated by metamorphism and crustal anatexis at greater depth, provides and explanation for the observed localized metamorphism and deformation at relatively high structural levels.

  7. Stratigraphy and structure of the Sevier thrust belt and proximal foreland-basin system in central Utah: A transect from the Sevier Desert to the Wasatch Plateau

    USGS Publications Warehouse

    Lawton, T.F.; Sprinkel, D.A.; Decelles, P.G.; Mitra, G.; Sussman, A.J.; Weiss, M.P.

    1997-01-01

    The Sevier orogenic belt in central Utah comprises four north-northwest trending thrust plates and two structural culminations that record crustal shortening and uplift in late Mesozoic and early Tertiary time. Synorogenic clastic rocks, mostly conglomerate and sandstone, exposed within the thrust belt were deposited in wedge-top and foredeep depozones within the proximal part of the foreland-basin system. The geologic relations preserved between thrust structures and synorogenic deposits demonstrate a foreland-breaking sequence of thrust deformation that was modified by minor out-of-sequence thrust displacement. Structural culminations in the interior part of the thrust belt deformed and uplifted some of the thrust sheets following their emplacement. Strata in the foreland basin indicate that the thrust sheets of central Utah were emplaced between latest Jurassic and Eocene time. The oldest strata of the foredeep depozone (Cedar Mountain Formation) are Neocomian and were derived from the hanging wall of the Canyon Range thrust. The foredeep depozone subsided most rapidly during Albian through Santonian or early Campanian time and accumulated about 2.5 km of conglomeratic strata (Indianola Group). The overlying North Horn Formation accumulated in a wedge-top basin from the Campanian to the Eocene and records propagation of the Gunnison thrust beneath the former foredeep. The Canyon Range Conglomerate of the Canyon Mountains, equivalent to the Indianola Group and the North Horn Formation, was deposited exclusively in a wedge-top setting on the Canyon Range and Pavant thrust sheets. This field trip, a three day, west-to-east traverse of the Sevier orogenic belt in central Utah, visits localities where timing of thrust structures is demonstrated by geometry of cross-cutting relations, growth strata associated with faults and folds, or deformation of foredeep deposits. Stops in the Canyon Mountains emphasize geometry of late structural culminations and relationships of

  8. 75 FR 20774 - Establishment of Class E Airspace; Mountain City, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-21

    ... FR 63976), Docket No. FAA-2009-0061; Airspace Docket No. 09-ASO-10. The FAA uses the direct final... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Mountain City, TN AGENCY... December 7, 2009 that establishes Class E airspace at Johnson County Airport, Mountain City, TN....

  9. Upper Oligocene evaporites in basin fill of Sevier Desert region, western Utah ( USA).

    USGS Publications Warehouse

    Lindsey, D.A.; Glanzman, R.K.; Naeser, C.W.; Nicholas, D.J.

    1981-01-01

    The basin fill beneath the Sevier Desert of western Utah contains evaporites that were deposited in a broad closed basin. All of the basin fill penetrated by the Gulf Oil 1 Gronning contains abundant volcanic detritus and its alteration products of Cenozoic age. Fission-track dating of tuffaceous sandstone yields ages of 26-28 m.y. Fossil pollen from mudstone in the evaporite-bearing strata includes forms no older than late Oligocene, in good agreement with the fission-track ages. Thus the age of evaporites is late Oligocene and younger. Anhydrite is present throughout approximately 900m of volcaniclastic rocks in the lower part of the section cut by the Gulf hole. Fossil pollen are indicative of an arid to semiarid flora.-from Authors

  10. 1. Elkmont vehicle bridge at Elkmont Campground, galvanized corrugated arch. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Elkmont vehicle bridge at Elkmont Campground, galvanized corrugated arch. - Great Smoky Mountains National Park Roads & Bridges, Elkmont Vehicle Bridge, Spanning Little River at Elkmont Campground, Gatlinburg, Sevier County, TN

  11. 19. Cantilevered barn in Cades Cove looking SSW. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Cantilevered barn in Cades Cove looking SSW. - Great Smoky Mountains National Park Roads & Bridges, Cades Cove Road & Laurel Creek Road, From Townsend Wye to Cades Cove, Gatlinburg, Sevier County, TN

  12. 1. View of Cades Cove Valley from first overlook on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Cades Cove Valley from first overlook on Rich Mountain Road looking S. - Great Smoky Mountains National Park Roads & Bridges, Rich Mountain Road, Between Cades Cove & park boundary at Rich Mountain Gap, Gatlinburg, Sevier County, TN

  13. 6. Roaring Fork Motor Nature Trail, road view after stop ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  14. 9. Roaring Fork Motor Nature Trail, Reagan House. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  15. 8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  16. 10. Reagan's Tub Mill and Roadway looking NE. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Reagan's Tub Mill and Roadway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  17. 5. Roaring Fork Motor Nature Trail, vista at stop three. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  18. 1. Roaring Fork Motor Nature Trail, entrance sign. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  19. 3. Roaring Fork Motor Nature Trail, view between second and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  20. 2. Roaring Fork Motor Nature Trail, road view before first ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  1. 13. Roadway and place of a thousand drips looking ESE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Roadway and place of a thousand drips looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  2. 7. Roaring Fork Motor Nature Trail, rocks along edge of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  3. Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  4. 15. Culvert and corrugated pipe with place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Culvert and corrugated pipe with place of a thousand drips in background looking S. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  5. 12. Roaring Fork Motor Nature Trail, place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  6. 11. Roaring Fork Motor Nature Trail, boulders along road after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  7. 17. Underside of bridge and abutment with large boulder looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Underside of bridge and abutment with large boulder looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  8. 16. Bridge at Old Road S looking ESE. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Bridge at Old Road S looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  9. 4. Overgrown overlook number 1 looking W. Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Overgrown overlook number 1 looking W. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  10. 18. Bridge over state route 321 on unopened section of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. Bridge over state route 321 on unopened section of Foothills Parkway in wear cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

  11. 9. Bridge over State Route 321 on unopened section of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Bridge over State Route 321 on unopened section of Foothills Parkway in wear cove looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

  12. 6. LITTLE GREENBRIER SCHOOL CEMETERY AND PARKING AREA LOOKING S, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. LITTLE GREENBRIER SCHOOL CEMETERY AND PARKING AREA LOOKING S, OFF SPUR FROM METCALF BOTTOMS TO WEAR COVE. - Great Smoky Mountains National Park Roads & Bridges, Little River Road, Between Sugarlands Visitor Center & Townsend Wye, Gatlinburg, Sevier County, TN

  13. 1. Deep Creek Road, picnic pavilion Great Smoky Mountains ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Deep Creek Road, picnic pavilion - Great Smoky Mountains National Park Roads & Bridges, Deep Creek Road, Between Park Boundary near Bryson City & Deep Creek Campground, Gatlinburg, Sevier County, TN

  14. 2. Deep Creek Road, old bridge at campground entrance. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Deep Creek Road, old bridge at campground entrance. - Great Smoky Mountains National Park Roads & Bridges, Deep Creek Road, Between Park Boundary near Bryson City & Deep Creek Campground, Gatlinburg, Sevier County, TN

  15. 10. Area of landslide on eastern section of Foothills Parkway ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Area of landslide on eastern section of Foothills Parkway looking NE. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

  16. 2. Big Creek Road, worm fence and road at trailhead. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Big Creek Road, worm fence and road at trailhead. - Great Smoky Mountains National Park Roads & Bridges, Big Creek Road, Between State Route 284 & Big Creek Campground, Gatlinburg, Sevier County, TN

  17. Testing the Extensional Detachment Paradigm: A Borehole Observatory in the Sevier Desert Basin, Utah

    NASA Astrophysics Data System (ADS)

    Christie-Blick, N.; Wernicke, B. P.

    2007-12-01

    The Sevier Desert basin, Utah represents a world-class target for scientific drilling and for the development of an in situ borehole observatory of active faulting, with potential for establishing that normal-sense slip can occur along a brittle low-angle fault and, by determining the conditions under which that may take place, for resolving the mechanical paradox associated with such structures. The Sevier Desert detachment was defined in the mid- 1970s on the basis seismic reflection data and commercial wells as the contact between Paleozoic carbonate rocks and Cenozoic basin fill over a depth range of ~0-4 km. Today, the interpreted fault is thought by most workers to root into the crust to the west, to have large estimated offset (< 47 km), to have been active over most of its history near its present 11° dip, and to be associated with contemporary surface extension (a 30- km-long zone of prominent Holocene fault scarps immediately west of Clear Lake). Although no seismicity has been documented on the detachment, its scale is consistent with earthquake magnitudes as large as M 7.0. A published alternative interpretation of the Paleozoic-Cenozoic contact as an unconformity rather than a fault has not been generally accepted. Deformation at detachment faults is commonly spatially restricted, and may have been missed in well cuttings. Exhumation of the detachment would have made it possible to remove critical footwall evidence prior to later sedimentary onlap, particularly at updip locations. The incomplete coverage and uneven quality of seismic reflection data on which the detachment interpretation depends, and an unresolved debate about stratigraphic ties to a critical well, leave room for discussion about interpretive details, including the possibility that deformation was distributed across several closely spaced faults. An apparent mismatch between stratigraphically based ages and fission-track evidence for the timing of footwall exhumation cannot be

  18. Upper Oligocene evaporites in basin fill of Sevier Desert region, western Utah

    SciTech Connect

    Lindsey, D.A.; Glanzman, R.K.; Naeser, C.W.; Nichols, D.J.

    1981-02-01

    The basin fill beneath the Sevier Desert of western Utah contains evaporites of late Oligocene age that were deposited in a broad closed basin. All of the basin fill penetrated by the Gulf Oil 1 Gronning (2458 m TD), one of only three deep holes in the basin, contains abundant volcanic detritus and its alteration products of Cenozoic age, when volcanism was intense in western Utah. Fission-track dating of tuffaceous sandstone, interbedded with evaporite minerals and representative of sandstone in the lower formations penetrated, yields ages of 26 to 28 m.y.; concordance of ages indicates no thermal resetting. Fossil pollen from mudstone in the evaporite-bearing strata includes forms no older than late Oligocene, in good agreement with the fission-track ages. Thus the age of the evaporites, and of all the basin fill penetrated by drilling, is late Oligocene and younger; previous work had assigned them ages ranging from Triassic to Eocene. Evaporite-bearing rocks were deposited during late Oligocene time in a broad closed basin under arid or semiarid conditions much like those of the Great Basin today. Anhydrite is present throughout approximately 900 m of volcaniclastic rocks in the lower part of the section cut by the Gulf hole, and more than 1500 m of anhydrite and halite is present in possibly equivalent strata in the nearby Argonaut dry hole. Fossil pollen from anhydrite-bearing rocks in the Gulf hole are indicative of an arid to semiarid flora, including plants similar to Mormon tea and possibly saltbush or buffalo berry. Evaporite minerals formed in the basin during dry periods; tuff was erupted from nearby volcanic centers, reworked by water, and deposited in the basin.

  19. Structural Discordance Between Neogene Detachments and Frontal Sevier Thrusts, Central Mormon Mountains, Southern Nevada

    NASA Astrophysics Data System (ADS)

    Wernicke, Brian; Walker, J. Douglas; Beaufait, Mark S.

    1985-02-01

    Detailed geologic mapping in the Mormon Mountains of southern Nevada provides significant insight into processes of extensional tectonics developed within older compressional orogens. A newly discovered, WSW-directed low-angle normal fault, the Mormon Peak detachment, juxtaposes the highest levels of the frontal most part of the east-vergent, Mesozoic Sevier thrust belt with autochthonous crystalline basement. Palinspastic analysis suggests that the detachment initially dipped 20-25° to the west and cut discordantly across thrust faults. Nearly complete lateral removal of the hanging wall from the area has exposed a 5 km thick longitudinal cross-section through the thrust belt in the footwall, while highly attenuated remnants of the hanging wall (nowhere more than a few hundred meters thick) structurally veneer the range. The present arched configuration of the detachment resulted in part from progressive "domino-style" rotation of a few degrees while it was active, but is largely due to rotation on younger, structurally lower, basement-penetrating normal faults that initiated at high-angle. The geometry and kinematics of normal faulting in the Mormon Mountains suggest that pre-existing thrust planes are not required for the initiation of low-angle normal faults, and even where closely overlapped by extensional tectonism, need not function as a primary control of detachment geometry. Caution must thus be exercised in interpreting low-angle normal faults of uncertain tectonic heritage such as those seen in the COCORP west-central Utah and BIRP's MOIST deep-reflection profiles. Although thrust fault reactivation has reasonably been shown to be the origin of a very few low-angle normal faults, our results indicate that it may not be as fundamental a component of orogenic architecture as it is now widely perceived to be. We conclude that while in many instances thrust fault reactivation may be both a plausible and attractive hypothesis, it may never be assumed.

  20. 3. BIG HOUSE (left) AND CORN CRIB (right) IN THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. BIG HOUSE (left) AND CORN CRIB (right) IN THE BACKGROUND. See also individual HABS documentation: Walker Family Farm, Big House (HABS No. TN-121 A), and Walker Family Farm, Corn Crib (HABS No. TN-121 C). - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

  1. Spatial and Temporal Distribution of Strain in the Sevier Desert Region from a Decade of BARGEN Continuous GPS Observations

    NASA Astrophysics Data System (ADS)

    Niemi, N. A.; Wernicke, B. P.

    2007-12-01

    A transect of four BARGEN GPS sites from 1997-2000 at latitude 39°N revealed a linear increase in velocity from 0 mm/yr on the Colorado Plateau to ~4 mm/yr in east-central Nevada. These geodetic data, when combined with paleoseismic and neotectonic observations, were used to argue for present-day strain accumulation on the Sevier Desert detachment, a seismically-imaged, low-angle (12°) normal fault that underlies a significant portion of west central Utah [Niemi et al., 2004]. A new GPS velocity solution [Davis et al., 2006], including 5 additional years of observations and a new GPS station in the eastern half of the transect, serves to both clarify and complicate our understanding of the spatial and temporal distribution of strain in this region. Geodetic velocity gradients among the three original eastern sites (from east to west, CAST, SMEL, and FOOT), and including the new site, SPIC, located between CAST and SMEL, continue to suggest a linear strain gradient from the Colorado Plateau to westernmost Utah, on a transect spanning the Wasatch, Sevier Desert, and House Range normal faults. In contrast, the baseline between FOOT and EGAN, which underwent extension from 1997-2000, began to contract in 2000, as site EGAN slowed with respect to the Colorado Plateau. This deviation in velocity continued until 2003, when EGAN began moving westward, and has, as of 2006, returned to near its pre-2000 velocity with respect to the Colorado Plateau. The eastward excursion of GPS site EGAN is not unique, and similar excursions are observed in the time series of all BARGEN GPS sites that lie west of ~114.25°W over the time period 2000-2003 [Davis et al., 2006]. The origin of this velocity anomaly is uncertain, but the magnitude and spatial extent of the excursion, as well as the observation of a deep earthquake swarm (~30-40 km depth) coincident with dramatic motion of GPS site SLID, near Lake Tahoe, in 2003 [Smith et al., 2004] suggest a deep crustal or mantle influence on

  2. [Proteolytic control of the antirestriction activity of Tn2l, Tn5053, Tn5045 Tn501 TN402 non-conjugative transposons].

    PubMed

    Zavilgelsky, G B; Kotova, V Yu; Melkina, O E; Balabanov, V P; Mindlin, S Z

    2015-01-01

    Conjugative plasmids and conjugative transposons contain the genes, which products specifically inhibit the type I restriction--modification systems. Here is shown that non-conjugative transposons Tn2l, Tn5053, Tn5045, Tn501, Tn402 partially inhibit the restriction activity of the type I restriction-modification endonuclease EcoKI (R2M2S1) in Escherichia coli cells K12 (the phenomenon of restriction alleviation, RA). Antirestriction activity of the transposons is determined by the MerR and ArdD proteins. Antirestriction activity of transposons is absent in mutants E. coli K12 clpX and clpP and is decreased in mutants E. coli K12 recA, recBC and dnaQ (mutD). Induction of the RA in response to the MerR and ArdD activities is consistent with the production of unmodified target sequences following DNA repair and DNA synthesis associated with recombination repair or replication errors. RA effect is determined by the ClpXP-dependent degradation of the endonuclease activity R subunit of EcoKI (R2M2S1). PMID:26065261

  3. A Fold Province in the Hinterland of the Sevier Orogenic Belt in Eastern Nevada: A Valley and Ridge in the Basin and Range

    NASA Astrophysics Data System (ADS)

    Long, S. P.

    2014-12-01

    The overprinting effects of Cenozoic extensional tectonism hinder analysis of the style, geometry, and timing of Cordilleran contractional deformation in the hinterland of the Sevier fold-thrust belt in Nevada. To address this problem, a 1:250,000-scale paleogeologic map of eastern Nevada, showing spatial distributions of Paleozoic-Mesozoic rocks exposed beneath a Paleogene unconformity, divided out at the formation scale, is integrated with maps of dip magnitude for Paleozoic-Mesozoic and Tertiary rocks, published sedimentary thickness records, and a published reconstruction of Basin and Range extension, in order to define and regionally-correlate thrust faults and folds, and estimate the pre-extensional amplitude, wavelength, and limb dips of folds. A new structural province, the Eastern Nevada fold belt (ENFB) is defined, and consists of a 100-150 km-wide region containing as many as five 1st-order, N-trending folds that can be traced for map distances between 100-250 km, have amplitudes between 2-4 km, wavelengths between 20-40 km, pre-extensional limb dips typically between 10-30˚, and deform rocks as young as Jurassic and Early Cretaceous. No regional-scale thrust faults or decollemont horizons breach modern exposure levels in the ENFB. ENFB folds are interpreted to have formed above a deep (≥10 km below the Paleogene unconformity), blind decollemont or shear zone, perhaps the westward projection of the master decollemont of the Sevier fold-thrust belt. Two additional structural provinces of the Sevier hinterland, the Central Nevada thrust belt (CNTB) and Western Utah thrust belt (WUTB), represent zones of N-striking, E-vergent thrust faults and folds that each accommodated ca. 10 km of shortening. Both thrust belts branch northward off of the Sevier thrust belt, and are interpreted to represent contemporary, interior components of the Sevier thrust system. The CNTB, WUTB, and the intervening ENFB collectively record low-magnitude (a few 10's of km) upper

  4. Cretaceous to early Tertiary paleogeography in the hinterland of the Sevier thrust belt, east-central Nevada

    SciTech Connect

    Vandervoort, D.S.; Schmitt, J.G. )

    1990-06-01

    The Cretaceous to early Tertiary hinterland of the Sevier thrust belt in east-central Nevada underwent two periods of sedimentary basin development. The older (Early Cretaceous) episode is characterized by basins that formed coeval with deformation within a belt of contractional structures. Basins were characterized by periods of through-flowing fluvial drainage. The younger (Late Cretaceous to Eocene) episode of basin development was characterized by internally drained alluvial basins that subsequently expanded into more regional lacustrine basins. Basal alluvial detritus indicates that basin inception occurred as a result of rapid development of topographic relief. Synsedimentary tectonism was characterized by horizontal extension due to attainment of equilibrium topography in response to crustal thickening.

  5. Rapid Intradeformational Emplacement of the Big Hole Canyon Pluton Into the Sevier Fold-Thrust Belt, Southwest Montana.

    NASA Astrophysics Data System (ADS)

    Hespenheide, M. A.

    2002-12-01

    The Big Hole Canyon pluton (BHCp) is a Late Cretaceous pluton emplaced within the Sevier fold-and-thrust belt of the western North American Cordillera. The pluton is exposed over 60km2 and a thickness of ~1400m. Combined anisotropy of magnetic susceptibility (AMS), structural, and field studies document a clear pattern of magmatic flow radiating from at least three subvertical conduits <100m wide and ~300 to ~800m long. Interpreted flow plunges change rapidly to subhorizontal fabrics across the rest of the pluton, matching the expected pattern for laccolithic emplacement. Ascent conduits within the Big Hole Canyon pluton are coincident with the fold axis of an anticline above a thrust ramp, suggesting that the magma ascended up the fault of the fault-bend-fold. Geobarometry and stratigraphic reconstructions indicate an emplacement depth of approximately ~3km. Preliminary thermal modeling indicates that the BHCp was emplaced in 250,000 years, likely between periods of regional shortening deformation. Rapid magma ascent rates calculated by dike flow modeling and implied by entrained wall-rock xenoliths may indicate sequential magma injection into the pluton; an absence of chill margins between phases within the pluton indicates that sequential injections must have taken place quickly enough that the magmas did not have time to cool below the solidus temperature. The geometry and location of the BHCp suggest that magma used a pre-existing fault as a mechanical discontinuity for both ascent and emplacement. Continued intrusion of magma had a sufficient amount of driving pressure to stretch, shear, and lift the roof of the pluton. Detailed field mapping, structural studies, AMS, and thermobarometry indicate that the Late Cretaceous Big Hole Canyon pluton was emplaced as a laccolith at the top of a pre-existing fault-bend-fold in the frontal portion of the Sevier fold-thrust belt.

  6. 76 FR 31885 - Prevailing Rate Systems; Redefinition of the Northern Mississippi and Memphis, TN, Appropriated...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    ... analysis of the regulatory criteria for defining appropriated fund FWS wage areas, we find that Panola... analysis, we recommend that Panola County be redefined to the Memphis wage area. The Federal Prevailing... Memphis, TN, Appropriated Fund Federal Wage System Wage Areas AGENCY: U.S. Office of Personnel...

  7. The Mormon Peak and Tule Springs Detachments of Southern Nevada and Their Role in Interpreting the Subsurface Structure of the Sevier Desert Basin

    NASA Astrophysics Data System (ADS)

    Wernicke, B.; Axen, G. J.

    2007-12-01

    The eastern Sevier front is commonly overprinted by west-dipping normal faults that have been active through much of Cenozoic time. Although examples of disparate age and geometry abound, the Sevier Desert detachment stands out as being: (1) a very large, low-angle structure, (2) probably still active, and (3) at drillable depth, and hence is a prime target for scientific drilling. Among the closest surface-exposed analogs of the detachment are the Miocene Mormon Peak and Tule Springs detachments in southern Nevada, about 250 km SSW of the Sevier Desert. In both areas, the detachments developed within a few km of the basal Sevier thrust. The few traces of the southern Nevada detachments that were identified by reconnaissance mapping in the 1950s were first interpreted as underlying "rootless" gravity-slide masses. Detailed stratigraphic and structural mapping in the 1980s revealed the full extent of these detachments and their close relationship to the basal Sevier thrust. Construction and retrodeformation of cross sections through the two detachments demonstrated that neither fault could represent the base of a surficial slide mass. The key field relationship is the structural contiguity of the hanging walls of the detachments with large mountain range blocks in their down-dip directions. "Rootless" gravity-driven masses of equivalent or larger scale are widely documented, and in contrast to the southern Nevada detachments show clear evidence along their down-dip portions of either riding over the earth's surface (e.g. Heart Mountain, WY; Shadow Valley, CA) or having been internally shortened (e.g. Bearpaw Mountains, MT; Louisiana shelf). A rootless slide origin for the Mormon Peak detachment has been revived based on meso- and microstructural analysis of the fault surface (refs. 1, 2, and 3), but the hypothesis still fails the retrodeformation test at macroscopic scale. The retrodefomed sections indicate that the footwalls of the southern Nevada detachments

  8. Complete Nucleotide Sequence of Tn10

    PubMed Central

    Chalmers, Ronald; Sewitz, Sven; Lipkow, Karen; Crellin, Paul

    2000-01-01

    The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein in Bacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protect Escherichia coli from the toxic effects of various metals. PMID:10781570

  9. Arrayed transposase-binding sequences on the ends of transposon Tn5090/Tn402.

    PubMed

    Kamali-Moghaddam, M; Sundström, L

    2001-02-15

    The transposon Tn5090/Tn402 encodes a 559 amino acid transposase, TniA, with a DDE motif. Gel mobility shifting and cleavage protection analysis with DNase I and hydroxyl radical probes revealed that TniA binds to multiple repeat sequences on either terminus of Tn5090/Tn402. Four of these TniA-binding 19mers occurred on the left-hand (t) end and two on the right-hand (i) end. Hydroxyl radical cleavage protection demonstrated the presence of 3-6 bp contact sequences on one face of the DNA helix. The binding pattern and organisation of repeats suggested parallels between Tn5090/Tn402 and Mu, which controls its transpositional activity in the assembly step of a higher order transpososome complex. The complex terminal structure and genes of transposase and nucleotide-binding proteins in tandem are hallmarks of the handful of Mu-like elements that are known to date. PMID:11160934

  10. Neotectonics of the Sevier Desert basin, Utah as seen through the lens of multi-scale geophysical investigations

    NASA Astrophysics Data System (ADS)

    McBride, John H.; Nelson, Stephen T.; Heiner, Brandon D.; Tingey, David G.; Morris, Thomas H.; Rey, Kevin A.

    2015-07-01

    Geophysical surveys conducted in the Clear Lake fault zone (Utah) constrain the interpretation of neotectonics and volcanism along a portion of the Colorado Plateau-Basin-and-Range Transition Zone. The fault zone lies within the Sevier Desert, a region marked by numerous Quaternary fault scarps and bimodal volcanism. Lavas as young as 660 a B.P. are usually cut by faults, and many scarps have significant offsets implying the potential for M 7 + earthquakes. The range of scarp heights and uncertainty in scarp-magnitude regressions suggest variations on the order of 0.75 moment magnitude units. In order to characterize the range of deformation styles in the Clear Lake fault zone, three fault scarps were investigated for their patterns of shallow (< 1 km depth) deformation. A suite of methods, including seismic P- and SH-wave surveys, ground-penetrating radar, auger holes, and surface geological mapping produces a more complete view of near-surface Quaternary deformation. Sub-surface faults are expressed as scarps at the ground surface, but with differing tectonic styles: (1) growth strata in the hanging wall of a fault imply aseismic creep; (2) buried lavas draped over buried paleo-scarps indicate multiple slip events; and (3) a complex fault zone is expressed as ramps that step-over from one scarp to another. On a larger scale, the results bear directly on estimating seismic and volcanic hazards. In one locality, evidence of aseismic creep suggests hazard is lower than that inferred from the exposed scarp height or length. By contrast, multiple slip events at other locations indicate that hazards could be underestimated if scarps are assumed to represent single slip events. Finally, the intimate spatial association of Quaternary volcanic vents and faults implies that volcanic and seismic hazards are interrelated, both resulting from crustal extension where eruptive events exploited faults as conduits in the shallow crust.

  11. Paleogeographic and paleotectonic setting of sedimentary basins in the Sevier thrust belt and hinterland, eastern Great Basin

    SciTech Connect

    Schmitt, J.G. . Dept. of Earth Sciences); Vandervoort, D.S. . Dept. of Geological Sciences); Suydam, J.D. . Dept. of Geology)

    1993-04-01

    The eastern Great Basin contains a sparse record of broadly distributed Cretaceous sedimentary rocks which record: evolution of intermontane basins during development of the Sevier (Sv)contractional orogen and incipient extensional collapse of the elevated Sv hinterland (east-central NV), and complex tectono-sedimentary interactions between frontal thrust belt structures and the western margin of the adjacent foreland basin. Palinspastic restoration of these strata and associated structures to pre-Tertiary extension positions reveals a clearer pictures of Cretaceous basin paleogeography and allows comparison with the Puna/Altiplano plateau and precordillera thrust belt of the Neogene Andean orogen. Two syntectonic stratal assemblages are present in east-central NV. Lower Cretaceous alluvial strata (Newark Canyon Fm) record basin development coeval with emergence of contractional structures in the Sv hinterland. Localized early Cretaceous basins were possibly piggyback immature; periods of open drainage to the to the east and south suggest connection with the nascent Sv foreland basin to the east (Cedar Mountain/Sanpete Fms) prior to major thrust loading in central Utah. Development of hinterland structures is almost recorded by Aptian-Albian foreland basin alluvial deposits in SW Utah (Dakota Fm) and southern Nevada (Willow Tank Fm). Upper Cretaceous to Eocene strata (Sheep Pass Fm) record inception of regionally abundant alluvial-lacustrine basins which developed in response to onset of latest Cretaceous extension and associated collapse of the Sv hinterland. Evolution of the structurally complex western margin of the Sv foreland basin is recorded in Cretaceous through Eocene strata deposited in: piggyback basins which were at times hydrologically connected to the adjacent foreland basins, and thrust-proximal portions of the foreland basin. These proximal areas are characterized by folding and faulting of basin fill and development of intrabasinal unconformities.

  12. Tn5 as an insect gene vector.

    PubMed

    Rowan, Kathryn H; Orsetti, Jamison; Atkinson, Peter W; O'Brochta, David A

    2004-07-01

    The purpose of this study was to explore alternatives to insect-derived transposable elements as insect gene vectors with the intention of improving existing insect transgenesis methods. The mobility properties of the bacterial transposon, Tn5, were tested in mosquitoes using a transient transposable element mobility assay and by attempting to create transgenic insects. Tn5 synaptic complexes were assembled in vitro in the absence of Mg(2+) and co-injected with a target plasmid into developing yellow fever mosquito, Aedes aegypti, embryos. Target plasmids recovered from embryos a day later were screened for the presence of Tn5. Recombinants (transposition events) were found at a frequency of 1.2 x 10(-3). Some transposition events did not appear to be associated with canonical 9 bp direct duplications at the site of insertion and also were associated with either deletions or rearrangements. A Tn5 element containing the brain-specific transgene, 3 x P3DsRed, was assembled into synaptic complexes in vitro and injected into pre-blastoderm embryos of Ae. aegypti. Of the approximately 900 embryos surviving injection and developing into adults, two produced transgenic progeny. Both transgenic events involved the co-integrations of approximately five elements resulting in nested and tandem arrayed Tn5::3 x P3DsRed elements. This study extends the known host range of Tn5 to insects and makes available to insect biologists and others another eukaryotic genome-manipulation tool. The hyperactivity of synaptic complexes may be responsible for the unusual clustering of elements and managing this aspect of the element's behavior will be important in future applications of this technology to insects. PMID:15242711

  13. An Early Cretaceous garnet pressure-temperature path recording synconvergent burial and exhumation from the hinterland of the Sevier orogenic belt, Albion Mountains, Idaho

    NASA Astrophysics Data System (ADS)

    Kelly, Eric D.; Hoisch, Thomas D.; Wells, Michael L.; Vervoort, Jeffrey D.; Beyene, Mengesha A.

    2015-08-01

    Rocks may undergo complex pressure-temperature ( P- T) histories during orogenesis in response to alternating episodes of synconvergent burial and exhumation. In this study, chemical zoning in garnets combined with textural and chemical evidence from the schist of Willow Creek in the Albion Mountains of south-central Idaho (USA), reveals a complex P- T path during the early stages of Sevier orogenesis. The distribution of quartz inclusions combined with internal resorption features establishes a hiatus in garnet growth. Chemical zoning was simulated using a G-minimization approach to yield a P- T path consisting of three distinct pressure changes during increasing temperature, defining an "N" shape. Lu-Hf isochron ages from multiple garnet fractions and whole-rock analyses in two samples are 132.1 ± 2.4 and 138.7 ± 3.5 Ma. The samples were collected from the hanging wall of the Basin-Elba thrust fault and yielded results similar to those previously obtained from the footwall. This leads to several conclusions: (1) Both the hanging wall and footwall experienced the same metamorphic event, (2) the paths document a previously unrecognized crustal thickening and synorogenic extension cycle that fills an important time gap in the shortening history of the Sevier retroarc, suggesting progressive eastward growth of the orogen rather than a two-stage history, and (3) episodes of extensional exhumation during protracted convergent orogenesis are increasingly well recognized and highlight the dynamic behavior of orogenic belts.

  14. 78 FR 48762 - Tennessee Disaster #TN-00076

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-09

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00076 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... Application Deadline Date: 05/02/2014. ADDRESSES: Submit completed loan applications to: U.S. Small...

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    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-25

    ... ADMINISTRATION Tennessee Disaster TN-00074 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY...: 11/14/2013. ADDRESSES: Submit completed loan applications to: U.S. Small Business Administration... CONTACT: A. Escobar, Office of Disaster Assistance, U.S. Small Business Administration, 409 3rd Street...

  16. 76 FR 28840 - Tennessee Disaster # TN-00053

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-18

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Disaster Declaration 12572 and 12573 Tennessee Disaster TN-00053 AGENCY: U.S. Small Business...-line, Winds, and Flooding. Incident Period: 04/19/2011 and continuing. Effective Date:...

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    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00063 AGENCY: Small Business Administration. ACTION: Notice. SUMMARY: This...-DR), dated 03/16/2012. Incident: Severe Storms, Tornadoes, Straight-line Winds, and...

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    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-10

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Notice SUMMARY...-1974-DR), dated 05/01/2011. Incident: Severe Storms, Tornadoes, Straight-line Winds, and...

  19. 76 FR 29284 - Tennessee Disaster #TN-00055

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00055 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of Tennessee (FEMA- 1979-DR), dated 05/09/2011. Incident: Severe Storms, Tornadoes,...

  20. 76 FR 27138 - Tennessee Disaster # TN-00052

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-10

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00052 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of Tennessee (FEMA- 1974-DR), dated 05/01/2011. Incident: Severe Storms, Tornadoes,...

  1. 75 FR 26815 - Tennessee Disaster # TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00039 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY...-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding, Straight-line Winds, and...

  2. 76 FR 29286 - Tennessee Disaster #TN-00054

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00054 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... Straight-line Winds. Incident Period: 04/04/2011. Effective Date: 05/09/2011. Physical Loan...

  3. 75 FR 26814 - Tennessee Disaster #TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00038 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of Tennessee (FEMA- 1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding,...

  4. 76 FR 52043 - Tennessee Disaster #TN-00056

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... ADMINISTRATION Tennessee Disaster TN-00056 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 08/12...: Tennessee: Campbell, Grainger, Hancock, Union. Kentucky: Bell, Whitley. Virginia: Lee. The Interest...

  5. 76 FR 19515 - Tennessee Disaster #TN-00050

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-07

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00050 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of Tennessee (FEMA- 1965-DR), dated 04/01/2011. Incident: Severe Storms, Tornadoes, and...

  6. 76 FR 45309 - Tennessee Disaster #TN-00058

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00058 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of Tennessee (FEMA- 4005-DR), dated 07/20/2011. Incident: Severe Storms, Straight-line...

  7. 75 FR 57997 - Tennessee Disaster # TN-00043

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-23

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00043 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of Tennessee (FEMA- 1937-DR), dated 09/15/2010. Incident: Severe Storms and Flooding....

  8. 76 FR 20433 - Tennessee Disaster #TN-00048

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... ADMINISTRATION Tennessee Disaster TN-00048 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is a an amendment of the Administrative declaration of a disaster for the State of Tennessee...'s disaster declaration in the State of Tennessee, dated 03/23/2011, is hereby amended to...

  9. [Construction of cTnC-linker-TnI (P) Genes, Expression of Fusion Protein and Preparation of Lyophilized Protein].

    PubMed

    Song, Xiaoli; Liu, Xiaoyun; Cai, Lei; Wu, Jianwei; Wang, Jihua

    2015-12-01

    In order to construct and express human cardiac troponin C-linker-troponin I(P) [ cTnC-linker-TnI(P)] fusion protein, detect its activity and prepare lyophilized protein, we searched the CDs of human cTnC and cTnI from GenBank, synthesized cTnC and cTnI(30-110aa) into cloning vector by a short DNA sequence coding for 15 neutral amino acid residues. pCold I-cTnC-linker-TnI(P) was constructed and transformed into E. coli BL21(DE3). Then, cTnC-linker-TnI(P) fusion protein was induced by isopropyl-β-D-thiogalactopyranoside (IPTG). Soluable expression of cTnC-linker-TnI(P) in prokaryotic system was successfully obtained. The fusion protein was purified by Ni²⁺ Sepharose 6 Fast Flow affinity chromatography with over 95% purity and prepared into lyophilized protein. The activity of purified cTnC-linker-TnI(P) and its lyophilized protein were detected by Wondfo Finecare™ cTnI Test. Lyophilized protein of cTnC-linker-TnI(P) was stable for 10 or more days at 37 °C and 4 or more months at 25 °C and 4 °C. The expression system established in this research is feasible and efficient. Lyophilized protein is stable enough to be provided as biological raw materials for further research. PMID:27079099

  10. Functional Divergence in Teleost Cardiac Troponin Paralogs Guides Variation in the Interaction of TnI Switch Region with TnC.

    PubMed

    Genge, Christine E; Stevens, Charles M; Davidson, William S; Singh, Gurpreet; Peter Tieleman, D; Tibbits, Glen F

    2016-01-01

    Gene duplication results in extra copies of genes that must coevolve with their interacting partners in multimeric protein complexes. The cardiac troponin (Tn) complex, containing TnC, TnI, and TnT, forms a distinct functional unit critical for the regulation of cardiac muscle contraction. In teleost fish, the function of the Tn complex is modified by the consequences of differential expression of paralogs in response to environmental thermal challenges. In this article, we focus on the interaction between TnI and TnC, coded for by genes that have independent evolutionary origins, but the co-operation of their protein products has necessitated coevolution. In this study, we characterize functional divergence of TnC and TnI paralogs, specifically the interrelated roles of regulatory subfunctionalization and structural subfunctionalization. We determined that differential paralog transcript expression in response to temperature acclimation results in three combinations of TnC and TnI in the zebrafish heart: TnC1a/TnI1.1, TnC1b/TnI1.1, and TnC1a/TnI1.5. Phylogenetic analysis of these highly conserved proteins identified functionally divergent residues in TnI and TnC. The structural and functional effect of these Tn combinations was modeled with molecular dynamics simulation to link divergent sites to changes in interaction strength. Functional divergence in TnI and TnC were not limited to the residues involved with TnC/TnI switch interaction, which emphasizes the complex nature of Tn function. Patterns in domain-specific divergent selection and interaction energies suggest that substitutions in the TnI switch region are crucial to modifying TnI/TnC function to maintain cardiac contraction with temperature changes. This integrative approach introduces Tn as a model of functional divergence that guides the coevolution of interacting proteins. PMID:26979795

  11. Functional Divergence in Teleost Cardiac Troponin Paralogs Guides Variation in the Interaction of TnI Switch Region with TnC

    PubMed Central

    Genge, Christine E.; Stevens, Charles M.; Davidson, William S.; Singh, Gurpreet; Peter Tieleman, D.; Tibbits, Glen F.

    2016-01-01

    Gene duplication results in extra copies of genes that must coevolve with their interacting partners in multimeric protein complexes. The cardiac troponin (Tn) complex, containing TnC, TnI, and TnT, forms a distinct functional unit critical for the regulation of cardiac muscle contraction. In teleost fish, the function of the Tn complex is modified by the consequences of differential expression of paralogs in response to environmental thermal challenges. In this article, we focus on the interaction between TnI and TnC, coded for by genes that have independent evolutionary origins, but the co-operation of their protein products has necessitated coevolution. In this study, we characterize functional divergence of TnC and TnI paralogs, specifically the interrelated roles of regulatory subfunctionalization and structural subfunctionalization. We determined that differential paralog transcript expression in response to temperature acclimation results in three combinations of TnC and TnI in the zebrafish heart: TnC1a/TnI1.1, TnC1b/TnI1.1, and TnC1a/TnI1.5. Phylogenetic analysis of these highly conserved proteins identified functionally divergent residues in TnI and TnC. The structural and functional effect of these Tn combinations was modeled with molecular dynamics simulation to link divergent sites to changes in interaction strength. Functional divergence in TnI and TnC were not limited to the residues involved with TnC/TnI switch interaction, which emphasizes the complex nature of Tn function. Patterns in domain-specific divergent selection and interaction energies suggest that substitutions in the TnI switch region are crucial to modifying TnI/TnC function to maintain cardiac contraction with temperature changes. This integrative approach introduces Tn as a model of functional divergence that guides the coevolution of interacting proteins. PMID:26979795

  12. Structural and microstructural evolution of the Rattlesnake Mountain Anticline (Wyoming, USA): New insights into the Sevier and Laramide orogenic stress build-up in the Bighorn Basin

    NASA Astrophysics Data System (ADS)

    Beaudoin, Nicolas; Leprêtre, Rémi; Bellahsen, Nicolas; Lacombe, Olivier; Amrouch, Khalid; Callot, Jean-Paul; Emmanuel, Laurent; Daniel, Jean-Marc

    2012-11-01

    The Rocky Mountains in western US provide among the best examples of thick-skinned tectonics: following a period of thin-skinned tectonics related to the Sevier orogeny, the compressional reactivation of basement faults gave birth to the so-called Laramide uplifts/arches. The Bighorn basin, located in Wyoming, is therefore a key place to study the transition from thin- to thick-skinned tectonics in orogenic forelands, especially in terms of microstructural and stress/strain evolution. Our study focuses on a classic Laramide structure: the Rattlesnake Mountain Anticline (RMA, Wyoming, USA), a basement-cored anticline located in the western part of the Bighorn basin. Stress and strain evolution analysis in folded sedimentary layers and underlying faulted basement rocks were performed on the basis of combined analyses of fractures, fault-slip data and calcite twinning paleopiezometry. Most of the fractures are related to three main tectonic events: the Sevier thin-skinned contraction, the Laramide thick-skinned contraction, and the Basin and Range extension. Serial balanced cross-sections of RMA and displacement profiles suggest that all thrust faults were coeval, evidencing strain distribution in the basement during faulting. The comparison of RMA with another structure located in the eastern edge of the Bighorn basin, i.e. the Sheep Mountain Anticline (SMA), allows to propose a conceptual model for the geometric and kinematic evolution of Laramide-related basement-cored anticlines. Finally, the stress evolution is reconstructed at both the fold scale and the basin scale. We show that the evolution of stress trends and magnitudes was quite similar in both structures (RMA and SMA) during Laramide times (thick-skinned tectonics), in spite of different stress regimes. During Sevier (thin-skinned tectonics) and post-Laramide times, stress trends and fracture patterns were different in these two structures. These results suggest that the distance to the orogenic front

  13. 14. Roaring Fork Motor Nature Trail, Place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  14. Magnitude, Timing, and Geometry of Extension in the Southern Sevier Desert Basin From Piercing Points, Seismic-Stratigraphic Reconstruction, and Deep well Data

    NASA Astrophysics Data System (ADS)

    Coogan, J. C.; Decelles, P. G.

    2007-12-01

    Palinspastic reconstruction of Mesozoic thrust sheets provides the main constraint for an estimated 47 km of Cenozoic extensional displacement along the Sevier Desert detachment (SDD) in the central Sevier Desert Basin. Hanging wall and footwall piercing points indicate that the SDD accommodated a minimum of 35 km of extensional displacement in the narrower southern part of the basin. The piercing points for the SDD are defined by the intersection of the SDD, the Canyon Range thrust (CRT), and a regional early Cenozoic erosion surface (ES). The hanging wall piercing point lies immediately northeast of the Cricket Mountains, where the SDD-CRT- ES intersection is narrowly defined by intersecting structure maps derived from published seismic reflection data. The footwall piercing point lies in the southern foothills of the Canyon Range, where the SDD breakaway plane is well constrained by an industry seismic line that lies within 2 km of the exposed intersection of the CRT with the base of the Oligocene Oak City Formation. Timing of extension in the southern Sevier Desert basin is constrained by a kinematic reconstruction of detachment and imbricate fault displacement, footwall uplift, and supradetachment sedimentation for Oligocene, Miocene, and Plio-Pleistocene seismic sequences. The reconstruction is centered on a seismic reflection and gravity interpretation along the published Pan Canadian profiles 2 and 3 that is tied to dated intervals in six industry wells. Fault restoration indicates that Oligocene and Miocene phases of slip each accounted for about 40 percent of the total displacement. Simultaneous backstripping of the Oligocene, Miocene, and Plio-Pleistocene supradetachment sequences records hanging wall subsidence simultaneous with footwall uplift, with a footwall burial history that is consistent with published Miocene apatite and zircon fission-track ages of footwall samples. The geometric evolution of the southern SDD extensional system is consistent

  15. 76 FR 55232 - Establishment of Class E Airspace; Copperhill, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ... Airspace at Copperhill, TN, to accommodate the new Area Navigation (RNAV) Global Positioning System (GPS... the controlled airspace required to ] accommodate the new Area Navigation (RNAV) Global Positioning... Class E airspace at Copperhill, TN (76 FR 35370) Docket No. FAA-2011-0402. Interested parties...

  16. The Role of Sialyl-Tn in Cancer

    PubMed Central

    Munkley, Jennifer

    2016-01-01

    Activation of an aberrant glycosylation pathway in cancer cells can lead to expression of the onco-foetal sialyl-Tn (sTn) antigen. STn is a truncated O-glycan containing a sialic acid α-2,6 linked to GalNAc α-O-Ser/Thr and is associated with an adverse outcome and poor prognosis in cancer patients. The biosynthesis of the sTn antigen has been linked to the expression of the sialytransferase ST6GalNAc1, and also to mutations in and loss of heterozygosity of the COSMC gene. sTn neo- or over-expression occurs in many types of epithelial cancer including gastric, colon, breast, lung, oesophageal, prostate and endometrial cancer. sTn is believed to be carried by a variety of glycoproteins and may influence protein function and be involved in tumour development. This review discusses how the role of sTn in cancer development and tumour cell invasiveness might be organ specific and occur through different mechanisms depending on each cancer type or subtype. As the sTn-antigen is expressed early in carcinogenesis targeting sTn in cancer may enable the targeting of tumours from the earliest stage. PMID:26927062

  17. 48 CFR 1201.301-72 - TAC or TN numbering.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false TAC or TN numbering. 1201... FEDERAL ACQUISITION REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered consecutively on a fiscal year basis beginning with number “01”...

  18. 48 CFR 1201.301-72 - TAC or TN numbering.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false TAC or TN numbering. 1201... FEDERAL ACQUISITION REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered consecutively on a fiscal year basis beginning with number “01”...

  19. 48 CFR 1201.301-72 - TAC or TN numbering.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false TAC or TN numbering. 1201... FEDERAL ACQUISITION REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered consecutively on a fiscal year basis beginning with number “01”...

  20. 48 CFR 1201.301-72 - TAC or TN numbering.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false TAC or TN numbering. 1201... FEDERAL ACQUISITION REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered consecutively on a fiscal year basis beginning with number “01”...

  1. 48 CFR 1201.301-72 - TAC or TN numbering.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false TAC or TN numbering. 1201... FEDERAL ACQUISITION REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered consecutively on a fiscal year basis beginning with number “01”...

  2. IntI2 Integron Integrase in Tn7

    PubMed Central

    Hansson, Karin; Sundström, Lars; Pelletier, Alex; Roy, Paul H.

    2002-01-01

    Integrons can insert and excise antibiotic resistance genes on plasmids in bacteria by site-specific recombination. Class 1 integrons code for an integrase, IntI1 (337 amino acids in length), and are generally borne on elements derived from Tn5090, such as that found in the central part of Tn21. A second class of integron is found on transposon Tn7 and its relatives. We have completed the sequence of the Tn7 integrase gene, intI2, which contains an internal stop codon. This codon was found to be conserved among intI2 genes on three other Tn7-like transposons harboring different cassettes. The predicted peptide sequence (IntI2*) is 325 amino acids long and is 46% identical to IntI1. In order to detect recombination activity, the internal stop codon at position 179 in the parental allele was changed to a triplet coding for glutamic acid. The sequences flanking the cassette arrays in the class 1 and 2 integrons are not closely related, but a common pool of mobile cassettes is used by the different integron classes; two of the three antibiotic resistance cassettes on Tn7 and its close relatives are also found in various class 1 integrons. We also observed a fourth excisable cassette downstream of those described previously in Tn7. The fourth cassette encodes a 165-amino-acid protein of unknown function with 6.5 contiguous repeats of a sequence coding for 7 amino acids. IntI2*179E promoted site-specific excision of each of the cassettes in Tn7 at different frequencies. The integrases from Tn21 and Tn7 showed limited cross-specificity in that IntI1 could excise all cassettes from both Tn21 and Tn7. However, we did not observe a corresponding excision of the aadA1 cassette from Tn21 by IntI2*179E. PMID:11872723

  3. Transposon Tn5 specifies streptomycin resistance in Rhizobium spp.

    PubMed Central

    Selvaraj, G; Iyer, V N

    1984-01-01

    Transposon Tn5 conferred streptomycin resistance on different strains of Rhizobium meliloti, Rhizobium leguminosarum, and Rhizobium trifolii but not on Escherichia coli. A gene (str) specifying this phenotype has been identified and localized on the physical and genetic map of Tn5. It is transcribed from the promoter of neo, the gene that encodes neomycin phosphotransferase. The str gene is downstream from neo in a single transcriptional unit, as revealed by molecular cloning of different segments of Tn5 and by cloning of the neo-str region of Tn5 downstream from a lac promoter. Fusion of the SalI-generated rightward segment of Tn5 (devoid of neo) to a part of a tetracycline resistance gene, tet, in a plasmid or downstream from a lac promoter in a plasmid resulted in significant levels of streptomycin resistance in an R. meliloti host, suggesting that the str gene product can function independent of neomycin phosphotransferase. A natural isolate of R. meliloti that does not express Tn5-associated streptomycin resistance has been identified. We have used the str of Tn5 as a genetic marker in Rhizobium spp. PMID:6327612

  4. Geologic Map of the Pahranagat Range 30' x 60' Quadrangle, Lincoln and Nye Counties, Nevada

    USGS Publications Warehouse

    Jayko, A.S.

    2007-01-01

    Introduction The Pahranagat Range 30' x 60' quadrangle lies within an arid, sparsely populated part of Lincoln and Nye Counties, southeastern Nevada. Much of the area is public land that includes the Desert National Wildlife Range, the Pahranagat National Wildlife Refuge, and the Nellis Air Force Base. The topography, typical of much of the Basin and Range Province, consists of north-south-trending ranges and intervening broad alluvial valleys. Elevations range from about 1,000 to 2,900 m. At the regional scale, the Pahranagat Range quadrangle lies within the Mesozoic and early Tertiary Sevier Fold-and-Thrust Belt and the Cenozoic Basin and Range Province. The quadrangle is underlain by a Proterozoic to Permian miogeoclinal section, a nonmarine clastic and volcanic section of middle Oligocene or older to late Miocene age, and alluvial deposits of late Cenozoic age. The structural features that are exposed reflect relatively shallow crustal deformation. Mesozoic deformation is dominated by thrust faults and asymmetric or open folds. Cenozoic deformation is dominated by faults that dip more than 45i and dominostyle tilted blocks. At least three major tectonic events have affected the area: Mesozoic (Sevier) folding and thrust faulting, pre-middle Oligocene extensional deformation, and late Cenozoic (mainly late Miocene to Holocene) extensional deformation. Continued tectonic activity is expressed in the Pahranagat Range area by seismicity and faults having scarps that cut alluvial deposits.

  5. 75 FR 27008 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: Small Business Administration. ACTION: Amendment 3... declaration remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James...

  6. 75 FR 35103 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: Small Business Administration. ACTION: Amendment 7... declaration remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James...

  7. 75 FR 35103 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 8... Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for...

  8. 75 FR 27009 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 1... remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E....

  9. 75 FR 27008 - TENNESSEE Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION TENNESSEE Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 3... unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera,...

  10. 75 FR 27009 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: Small Business Administration. ACTION: Amendment 2... Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance....

  11. 75 FR 27010 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 2... Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance. BILLING...

  12. Style and magnitude of Mesozoic thrust faulting in the hinterland of the Sevier thrust belt Pequop Mountains-Wood Hills-East Humboldt Range region, northeast Nevada

    SciTech Connect

    Camilleri, P.A. . Dept. of Geology and Geophysics)

    1993-04-01

    The Pequop Mountains (PM), Wood Hills (WH) and East Humboldt Range (EHR), NE Nevada, provide evidence that the hinterland of the Sevier thrust belt experienced large-magnitude Mesozoic shortening ([>=]55 km) and crustal thickening ([>=] 30 km). These ranges expose a structurally continuous crustal cross section of unmetamorphosed to high pressure upper amphibolite facies Triassic to Precambrian miogeoclinal strata. This sequence lies structurally beneath unmetamorphosed extensional klippen that omit metamorphic grade and crustal section, but also repeat stratigraphic units. Because they repeat stratigraphic units, the underlying miogeoclinal section, or footwall, must have once lain beneath a thrust fault (herein named the Windermere thrust). The footwall of the Windermere thrust was exhumed by two generations of top-to-the-W-NW low-angle normal faults that are distinguished by whether they are depositionally overlapped by Eocene volcanic rocks or if they cut the volcanic rocks in their hanging walls. The latter phase is associated with development of the mid-Tertiary extensional mylonitic shear zone in the EHR. An integration of geobarometric, metamorphic, and map data suggest (1) a NW dip of the footwall of the Windermere thrust with metamorphic facies belts trending perpendicular to dip direction and metamorphic grade increasing down dip, and (2) a top-to-the-SE sense-of-slip for the Windermere thrust. Assuming that the Windermere thrust comprised a flat on the youngest rocks exposed in the footwall (Triassic), the Mesozoic depth to the Windermere thrust in the northern PM is [>=] 7 km, in WH is [approximately]10--16 km, and in the EHR[>=]30 km. The Windermere thrust accommodated a minimum of 50 km of shortening associated with the Independence thrust is [>=] 5 km. These data indicate that the amount of hinterland shortening in NE Nevada greatly exceeds that to the south in the Eureka belt.

  13. Thermal evolution of a portion of the Sevier hinterland: The northern Ruby Mountains - east Humboldt Range and Wood Hills, northwestern Nevada

    NASA Astrophysics Data System (ADS)

    Hodges, K. V.; Snoke, A. W.; Hurlow, H. A.

    1992-02-01

    Conventional thermobarometry and Gibbs' Method modeling have been used to obtain new information pertaining to the Mesozoic-Tertiary thermal evolution of the Ruby Mountains-East Humboldt Range and Wood Hills metamorphic complexes, northeastern Nevada. Kyanite-grade assemblages from Clover Hill, in the extreme northeastern part of the East Humboldt Range, were produced at temperatures of 780-810 K and depths in excess of 35 km; previously published geochronologic data suggest an Early Cretaceous or Late Jurassic age for this event. The Clover Hill area subsequently experienced a major unroofing interval prior to Late Cretaceous time, during which it was brought roughly 20 km closer to the surface. The lack of stratigraphic evidence for substantial Cretaceous erosion in the region and the topology of the pressure-temperature paths for the Clover Hill samples suggest that the unroofing mechanism may have been tectonic denudation related to the relaxation of large topographic gradients produced by crustal thickening in the hinterland of the Sevier orogen. Metamorphic rocks at Clover Hill and within the Wood Hills core complex equilibrated at 820-900 K and 500-640 MPa (18-24 km) in Cretaceous time, prior to 115 Ma. Other parts of the Ruby Mountains-East Humboldt Range metamorphic complex, presently structurally separated from the Clover Hill sequence, were intruded extensively by Late Cretaceous granitic magmas and attained substantially higher temperatures than the Clover Hill-Wood Hills block. Sillimanite-grade assemblages from these areas apparently underwent roughly 10 km of unroofing and 130 K of cooling prior to final equilibration at 820-920 K and 360-430 MPa (13-16 km) during Oligocene extension.

  14. Structural and Kinetic Effects of PAK3 phosphorylation mimic of cTnI(S151E) on the cTnC-cTnI Interaction in the Cardiac Thin Filament

    PubMed Central

    Ouyang, Yexin; Mamidi, Ranganath; Jayasundar, Jayant James; Chandra, Murali; Dong, Wen-Ji

    2010-01-01

    Residue Ser151 of cTnI is known to be phosphorylated by p21-activated kinase 3 (PAK3). It has been found that PAK3-mediated phosphorylation of cTnI induces an increase in myofilament Ca2+ sensitivity, but the detailed mechanism is unknown. We investigated how the structural and kinetic effects mediated by pseudo-phosphorylation of cTnI (S151E) modulates Ca2+-induced activation of cardiac thin filaments. Using steady-state, time-resolved Förster Resonance Energy Transfer (FRET) and stopped-flow kinetic measurements, we monitored Ca2+-induced changes in cTnI-cTnC interactions. Measurements were done using reconstituted thin filaments, which contained the pseudo-phosphorylated cTnI(S151E). We hypothesized that the thin filament regulation is modulated by altered cTnC-cTnI interactions due to charge modification caused by the phosphorylation of Ser151 in cTnI. Our results showed that the pseudo-phosphorylation of cTnI (S151E) sensitizes structural changes to Ca2+ by shortening the intersite distances between cTnC and cTnI. Furthermore, kinetic rates of Ca2+ dissociation-induced structural change in the regulatory region of cTnI were significantly reduced by cTnI (S151E). The aforementioned effects of pseudo-phosphorylation of cTnI were similar to the effects of strong crossbridges on structural changes in cTnI. Our results provide novel information on how cardiac thin filament regulation is modulated by PAK3 phosphorylation of cTnI. PMID:20540949

  15. The Lower Sevier River Basin Crop Monitor and Forecast Decision Support System: Exploiting Landsat Imagery to Provide Continuous Information to Farmers and Water Managers

    NASA Astrophysics Data System (ADS)

    Torres-Rua, A. F.; Walker, W. R.; McKee, M.

    2013-12-01

    The last century has seen a large number of innovations in agriculture such as better policies for water control and management, upgraded water conveyance, irrigation, distribution, and monitoring systems, and better weather forecasting products. In spite of this, irrigation management and irrigation water deliveries by farmers/water managers is still based on factors like water share amounts, tradition, and past experience on irrigation. These factors are not necessarily related to the actual crop water use; they are followed because of the absence of related information provided in a timely manner at an affordable cost. Thus, it is necessary to develop means to deliver continuous and personalized information about crop water requirements to water users/managers at the field and irrigation system levels so managers at these levels can better quantify the required versus available water for irrigation during the irrigation season. This study presents a new decision support system (DSS) platform that addresses the absence of information on actual crop water requirements and crop performance by providing continuous updated farm-based crop water use along with other farm performance indicators such as crop yield and farm management to irrigators and water managers. This DSS exploits the periodicity of the Landsat Satellite Mission (8 to 16 days, depending on the period of interest) to provide remote monitoring at the individual field and irrigation system levels. The Landsat satellite images are converted into information about crop water use, yield performance and field management through application of state-of-the-art semi-physical and statistical algorithms that provide this information at a pixel basis that are ultimately aggregated to field and irrigation system levels. A version of the DSS has been implemented for the agricultural lands in the Lower Sevier River, Utah, and has been operational since the beginning of the 2013 irrigation season. The main goal of

  16. Draft Genome Sequence of Aeromonas hydrophila TN97-08.

    PubMed

    Tekedar, Hasan C; Kumru, Salih; Karsi, Attila; Waldbieser, Geoffrey C; Sonstegard, Tad; Schroeder, Steven G; Liles, Mark R; Griffin, Matt J; Lawrence, Mark L

    2016-01-01

    Aeromonas hydrophila is an opportunistic pathogen residing in freshwater environments that causes infection in fish and mammals. Here, we report the draft genome sequence of A. hydrophila strain TN97-08 isolated from a diseased bluegill (Lepomis macrochirus) in 1997. PMID:27231367

  17. 77 FR 28246 - Amendment of Class E Airspace; Tullahoma, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-14

    ... amend Class E airspace at Tullahoma, TN (77 FR 12759). Interested parties were invited to participate in... Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a... follows: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963...

  18. Draft Genome Sequence of Aeromonas hydrophila TN97-08

    PubMed Central

    Tekedar, Hasan C.; Kumru, Salih; Karsi, Attila; Waldbieser, Geoffrey C.; Sonstegard, Tad; Schroeder, Steven G.; Liles, Mark R.; Griffin, Matt J.

    2016-01-01

    Aeromonas hydrophila is an opportunistic pathogen residing in freshwater environments that causes infection in fish and mammals. Here, we report the draft genome sequence of A. hydrophila strain TN97-08 isolated from a diseased bluegill (Lepomis macrochirus) in 1997. PMID:27231367

  19. 78 FR 38555 - Amendment of Class E Airspace; Selmer, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-27

    ...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389..., TN. (78 FR 16202). Interested parties were invited to participate in this rulemaking effort by... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a Regulatory...

  20. 77 FR 40489 - Amendment of Class E Airspace; Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-10

    ... amend Class E airspace at Memphis, TN (77 FR 17360). Interested parties were invited to participate in... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a Regulatory Evaluation...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p....

  1. 76 FR 36166 - Tennessee Disaster Number TN-00051

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ... From the Federal Register Online via the Government Publishing Office U.S. SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4...: Submit completed loan applications to: U.S. Small Business Administration, Processing and...

  2. 76 FR 1512 - Amendment of Class E Airspace; Savannah, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... E airspace 700 feet above the surface, at Savannah, TN (75 FR 65584) Docket No. FAA-2010-1047... Executive Order 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR... FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The incorporation by reference in 14 CFR...

  3. 75 FR 27009 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance....

  4. 76 FR 48939 - Tennessee Disaster Number TN-00058

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00058 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for...

  5. 78 FR 31840 - Drawbridge Operation Regulation; Cumberland River, Nashville, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-28

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulation; Cumberland River, Nashville, TN... Nashville Railroad Drawbridge across the Cumberland River, mile 190.4, at Nashville, Tennessee. The... the Cumberland River, mile 190.4, at Nashville, Tennessee to remain in the...

  6. Iceland: Grímsvötn Volcano

    Atmospheric Science Data Center

    2013-04-17

    article title:  Grímsvötn Volcano Injects Ash into the Stratosphere     ... p.m. local time (1730 UTC) on Saturday, May 21, 2011. The volcano, located approximately 140 miles (220 kilometers) east of the capital ...

  7. 76 FR 33806 - Tennessee Disaster Number TN-00053

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00053 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Tennessee (FEMA-1979-DR), dated 05/09/ 2011. Incident: Severe Storms, Tornadoes, Straight-line Winds,...

  8. 76 FR 33806 - Tennessee Disaster Number TN-00051

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 3... Tennessee (FEMA-1974-DR), dated 05/01/ 2011. Incident: Severe Storms, Tornadoes, Straight-line Winds,...

  9. 76 FR 33805 - Tennessee Disaster Number TN-00055

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00055 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1..., Straight-line, Winds, and Flooding. Incident Period: 04/19/2011 and continuing. Effective Date:...

  10. 76 FR 33395 - Tennessee Disaster Number TN-00051

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Tennessee (FEMA-1974-DR), dated 05/01/ 2011. Incident: Severe Storms, Tornadoes, Straight-line Winds,...

  11. 76 FR 33395 - Tennessee; Disaster Number TN-00052

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee; Disaster Number TN-00052 AGENCY: U.S. Small Business Administration. ACTION: Amendment..., Tornadoes, Straight-line Winds, and Associated Flooding. Incident Period: 04/25/2011 through...

  12. 76 FR 36165 - Tennessee Disaster Number TN-00053

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ... From the Federal Register Online via the Government Publishing Office ] SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00053 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4... Tennessee (FEMA-1979-DR), dated 05/09/ 2011. Incident: Severe Storms, Tornadoes, Straight-line, Winds,...

  13. 76 FR 33805 - Tennessee Disaster Number TN-00052

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster Number TN-00052 AGENCY: U.S. Small Business Administration . ACTION: Amendment..., Tornadoes, Straight-line Winds, and Associated Flooding. Incident Period: 04/25/2011 through...

  14. Swift follow-up of SPIRITS16tn

    NASA Astrophysics Data System (ADS)

    Adams, S. M.; Jencson, J. E.; Kasliwal, M. M.

    2016-08-01

    We report Swift UVOT observations of SPIRITS16tn, a possible supernova in M108 (Jencson et al. ATel #9434). No source is detected in V, B, and U images taken on 2016-08-29.08 UT (14.0 days since SST discovery images) with exposure times of 540, 580, and 540s respectively.

  15. 75 FR 27846 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ... ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4... Tennessee (FEMA-1909-DR), dated 05/04/ 2010. Incident: Severe Storms, Flooding, Straight-line Winds, and.... SUPPLEMENTARY INFORMATION: The notice of the Presidential disaster declaration for the State of Tennessee,...

  16. 75 FR 30873 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ... ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: U.S. Small Business Administration. ACTION: Amendment 7... Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010 . Incident: Severe Storms, Flooding... major disaster declaration for Private Non-Profit organizations in the State of Tennessee, dated...

  17. 75 FR 27845 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ... ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4... Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding... disaster declaration for Private Non-Profit organizations in the State of Tennessee, dated 05/04/2010,...

  18. 75 FR 30870 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ... ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: U.S. Small Business Administration. ACTION: Amendment 6... Tennessee (FEMA-1909-DR), dated 05/04/ 2010 . Incident: Severe Storms, Flooding, Straight-line Winds, and... the State of Tennessee, dated 05/04/2010 is hereby amended to establish the incident period for...

  19. 76 FR 32387 - Tennessee Disaster Number TN-00053

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-06

    ... ADMINISTRATION Tennessee Disaster Number TN-00053 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Tennessee (FEMA-1979-DR), dated 05/09/ 2011. Incident: Severe Storms, Tornadoes, Straight-line, Winds, and.... SUPPLEMENTARY INFORMATION: The notice of the Presidential disaster declaration for the State of Tennessee,...

  20. 76 FR 35261 - Tennessee Disaster Number TN-00052

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-16

    ... ADMINISTRATION Tennessee Disaster Number TN-00052 AGENCY: U.S. Small Business Administration. ACTION: Amendment 3... Only for the State of Tennessee (FEMA-1974-DR), dated 05/01/2011. Incident: Severe Storms, Tornadoes... major disaster declaration for Private Non-Profit organizations in the State of Tennessee, dated...

  1. 75 FR 38155 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ... ADMINISTRATION Tennessee Disaster Number TN-00039 AGENCY: U.S. Small Business Administration. ACTION: Amendment 8... Tennessee (FEMA-1909-DR), dated 05/04/ 2010. Incident: Severe Storms, Flooding, Straight-line Winds, and... State of Tennessee, dated 05/04/2010 is hereby amended to extend the deadline for filing...

  2. 76 FR 35260 - Tennessee Disaster Number TN-00053

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-16

    ... ADMINISTRATION Tennessee Disaster Number TN-00053 AGENCY: U.S. Small Business Administration. ACTION: Amendment 3... Tennessee (FEMA-1979-DR), dated 05/09/ 2011. Incident: Severe Storms, Tornadoes, Straight-line, Winds, and... the State of Tennessee, dated 05/09/2011 is hereby amended to establish the incident period for...

  3. 76 FR 27739 - Tennessee Disaster Number TN-00051

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-12

    ... ADMINISTRATION Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Tennessee (FEMA-1974-DR), dated 05/01/ 2011. Incident: Severe storms, tornadoes, straight-line winds, and... State of Tennessee, dated 05/01/2011 is hereby amended to include the following areas as...

  4. 76 FR 35260 - Tennessee Disaster Number TN-00055

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-16

    ... ADMINISTRATION Tennessee Disaster Number TN-00055 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Only for the State of Tennessee (FEMA-1979-DR), dated 05/09/2011. Incident: Severe Storms, Tornadoes... major disaster declaration for Private Non-Profit organizations in the State of Tennessee, dated...

  5. 75 FR 29591 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-26

    ... ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 5... Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding... disaster declaration for Private Non-Profit organizations in the State of TENNESSEE, dated 05/04/2010,...

  6. 75 FR 30874 - Tennessee Disaster Number TN-00038

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ... ADMINISTRATION Tennessee Disaster Number TN-00038 AGENCY: U.S. Small Business Administration. ACTION: Amendment 6... Only for the State of Tennessee (FEMA-1909-DR), dated 05/04/2010. Incident: Severe Storms, Flooding... major disaster declaration for Private Non-Profit organizations in the State of Tennessee, dated...

  7. 75 FR 29590 - Tennessee Disaster Number TN-00039

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-26

    .... Contiguous Counties: (Economic Injury Loans Only): Alabama: Limestone. Tennessee: Lincoln. All other... 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance. BILLING CODE...

  8. Iceland's Grímsvötn volcano erupts

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    2011-05-01

    About 13 months after Iceland's Eyjafjallajökull volcano began erupting on 14 April 2010, which led to extensive air traffic closures over Europe, Grímsvötn volcano in southeastern took its turn. Iceland's most active volcano, which last erupted in 2004 and lies largely beneath the Vatnajökull ice cap, began its eruption activity on 21 May, with the ash plume initially reaching about 20 kilometers in altitude, according to the Icelandic Meteorological Office. Volcanic ash from Grímsvötn has cancelled hundreds of airplane flights and prompted U.S. president Barack Obama to cut short his visit to Ireland. As Eos went to press, activity at the volcano was beginning to subside.

  9. 75 FR 6257 - Watts Bar Reservoir Land Management Plan, Loudon, Meigs, Rhea, and Roane Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ...This notice is provided in accordance with the Council on Environmental Quality's regulations (40 CFR parts 1500 to 1508) and TVA's procedures for implementing the National Environmental Policy Act (NEPA). TVA has updated its 1988 land management plan (1988 Plan) for 16,036 acres of TVA public land on Watts Bar Reservoir in Tennessee. On November 19, 2009, the TVA Board of Directors (TVA......

  10. 75 FR 32201 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ... started the process through a notice in the Federal Register on April 2, 2008 (73 FR 17994). On December... we would conduct population and habitat surveys to evaluate shorebird use and invertebrate densities... more baseline inventories for non-game mammals, reptiles, amphibians and invertebrates....

  11. 76 FR 5194 - Tennessee National Wildlife Refuge, Henry, Benton, Decatur, and Humphreys Counties, TN; Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ... (73 FR 17994). On December 28, 1945, President Harry S. Truman signed Executive Order No. 9670... period via Federal Register notice on June 7, 2010 (75 FR 32201). We received 43 comments on the Draft... mammals, fish, reptiles, and amphibians. We announce our decision and the availability of the final...

  12. Lightcurve Analysis of Near-Earth Asteroid 2010 TN54

    NASA Astrophysics Data System (ADS)

    Warner, Brian D.; Benishek, Vladimir; Ferrero, Andrea

    2014-01-01

    CCD photometry observations of the near-Earth asteroid (NEA) 2010 TN 54 indicate a period of either 6.14 h or 12.12 h, depending on whether a monomodal or bimodal lightcurve is adopted. The amplitude was only 0.07 ± 0.01 mag, which - along with the period being nearly commensurate with an Earth day - made finding a definitive solution difficult, despite being observed from locations in North America and Europe.

  13. 8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY VIEW OF ABUTMENT SUPPORT INTO THE GROUND - Lost Creek Road Bridge, Spanning Sevier River at County Road 243, 2 miles east of Aurora, Aurora, Sevier County, UT

  14. Characterization of Tn6238 with a New Allele of aac(6′)-Ib-cr

    PubMed Central

    Quiroga, María P.; Orman, Betina; Errecalde, Laura; Kaufman, Sara

    2015-01-01

    Here, we report that the genetic structure of Tn1331 remained conserved in Argentina from 1989 to 2013 (72 of 73 isolates), with the exception being the plasmid-borne Tn1331-like transposon Tn6238 containing a new aac(6′)-Ib-cr allele recovered from a colistin-resistant Klebsiella pneumoniae clinical isolate. A bioinformatic analysis of aac(6′)-Ib-like gene cassettes suggests that this new aac(6′)-Ib-cr allele emerged through mutation or homologous recombination in the Tn1331 genetic platform. Tn6238 is a novel platform for the dissemination of aminoglycoside and fluoroquinolone resistance determinants. PMID:25691640

  15. Characterization of trimethoprim resistance by use of probes specific for transposon Tn7.

    PubMed Central

    Pulkkinen, L; Huovinen, P; Vuorio, E; Toivanen, P

    1984-01-01

    Transposon Tn7 codes for resistance to trimethoprim and streptomycin. For detection of Tn7 by DNA-DNA hybridization, two recombinant plasmids were constructed. The former contained a 1-kilobase BamHI fragment and the latter contained a 4.3-kilobase EcoRI-BamHI fragment of Tn7. These DNA fragments, which did not include the drug resistance genes, were used as probes for detecting Tn7-like sequences in bacterial strains by colony hybridization. They hybridized strongly to bacterial DNA known to carry Tn7 but not to DNA known to carry transposons other than Tn7. These probes were used to study the occurrence of Tn7 in bacterial strains isolated in the Turku City Hospital in Finland. Transposon Tn7 was present in 47.2% of 199 trimethoprim-resistant enterobacteria (MIC greater than or equal to 8 micrograms/ml). Among the 69 Proteus mirabilis strains studied, 75% contained Tn7, although none of these strains transferred trimethoprim resistance in conjugation tests. The reliability of colony hybridization was further confirmed by Southern hybridization to detect the Tn7-specific 2.6-kilobase HindIII restriction fragment. Colony hybridization proved to be a sensitive and rapid method for detecting Tn7-determined sequences. Images PMID:6089656

  16. Tn5393d, a complex Tn5393 derivative carrying the PER-1 extended-spectrum beta-lactamase gene and other resistance determinants.

    PubMed

    Mantengoli, Elisabetta; Rossolini, Gian Maria

    2005-08-01

    In Alcaligenes faecalis FL-424/98, a clinical isolate that produces the PER-1 extended-spectrum beta-lactamase, the bla(PER-1) gene was found to be carried on a 44-kb nonconjugative plasmid, named pFL424, that was transferred to Escherichia coli by electroporation. Investigation of the genetic context of the bla(PER-1) gene in pFL424 by means of a combined cloning and PCR mapping approach revealed that the gene is associated with a transposonlike element of the Tn3 family. This 14-kb element is a Tn5393 derivative of original structure, named Tn5393d, which contains the transposition module and the strAB genes typical of other members of the Tn5393 lineage plus additional resistance determinants, including the bla(PER-1) gene and a new allelic variant of the aphA6 aminoglycoside phosphotransferase gene, named aphA6b, whose product is active against kanamycin, streptomycin, and amikacin. Tn5393d apparently originated from the consecutive insertion of two composite transposons into a Tn5393 backbone carrying the aphA6b and the bla(PER-1) genes, respectively. The putative composite transposon carrying bla(PER-1), named Tn4176, is made of two original and nonidentical insertion sequences of the IS4 family, named IS1387a and IS1387b, of which one is interrupted by the insertion of an original insertion sequence of the IS30 family, named IS1066. In pFL424, Tn5393d is inserted into a Tn501-like mercury resistance transposon. Transposition of Tn5393d or modules thereof containing the bla(PER-1) gene from pFL424 to small multicopy plasmids or to a bacterial artificial chromosome was not detected in an E. coli host harboring both replicons. PMID:16048938

  17. Tn292l, a transposon encoding fosfomycin resistance.

    PubMed Central

    García-Lobo, J M; Ortiz, J M

    1982-01-01

    The determinant of resistance to fosfomycin of the Serratia marcescens plasmid pOU900 was transposed into the plasmid ColE1 and into the plasmid RP4 in the absence of the RecA function of the host. In each case, the acquisition of fosfomycin resistance was correlated with the presence of a discrete piece of DNA, uniform in size and in restriction pattern, This new, 7.5-megadalton transposable element encoding resistance to fosfomycin has been called Tn2921. A preliminary map of the transposon is presented. Images PMID:6282810

  18. Biodegradation of didecyldimethylammonium chloride by Pseudomonas fluorescens TN4 isolated from activated sludge.

    PubMed

    Nishihara, T; Okamoto, T; Nishiyama, N

    2000-04-01

    Bacteria that degrade didecyldimethylammonium chloride (DDAC) were isolated from activated sludge from a municipal sewage treatment plant by enrichment culture with DDAC as a sole carbon source. One of the isolates, Pseudomonas fluorescens TN4, degraded DDAC to produce decyldimethylamine and subsequently, dimethylamine, as the intermediates. The TN4 strain also assimilated the other quaternary ammonium compounds (QACs), alkyltrimethyl- and alkylbenzyldimethyl-ammonium salts, but not alkylpyridinium salts. TN4 was highly resistant to these QACs and degraded them by an N-dealkylation process. These data mean that there are some QAC-resistant and QAC-degrading bacteria such as TN4 in the environment. PMID:10792522

  19. New Sr, Nd, and Pb isotopic data from plutons in the northern Great Basin: Implications for crustal structure and granite petrogenesis in the hinterland of the Sevier thrust belt

    SciTech Connect

    Wright, J.E. ); Wooden, J.L. )

    1991-05-01

    The influence of tectonic setting and age on the variation of isotopic signatures of granitic plutons in the northern Great Basin has, in general, not been apparent from previous investigations. None of these studies revealed the remarkable correlation shown by this expanded Sr, Nd, and Pb isotopic data base. Jurassic-Early Cretaceous plutons in the northern Great Basin have a limited range of Sr and Nd isotopic values that cluster near bulk earth. Construction of Sr 0.706 and {epsilon}{sub Nd} = {minus}7 isotopic boundaries is virtually impossible for plutons of this age range. In contrast, Upper Cretaceous peraluminous granites east of the {epsilon}{sub Nd} = {minus}7 line have very negative {epsilon}{sub Nd} values and high initial Sr ratios, and they appear to represent essentially pure crustal melts. The data favor a model that equates generation of these plutons via crustal thickening associated with the Sevier thrust belt. Cenozoic plutons appear to be mixtures of mantle and crustal reservoirs, and their isotopic systematics, along with those of the Late Cretaceous age plutonic suite, define a previously unrecognized, approximately east-west-trending crustal boundary between predominantly Archean crust to the north and predominantly Proterozoic crust to the south. The isotopic data from the Jurassic-Early Cretaceous plutonic suite do not reflect the presence of this boundary, suggesting that the isotopic systematics of this plutonic suite may not have been controlled by the same variations in crustal and/or mantle lithospheric structure at depth.

  20. Properties of the Translocatable Tetracycline-Resistance Element Tn10 in ESCHERICHIA COLI and Bacteriophage Lambda

    PubMed Central

    Kleckner, Nancy; Barker, David F.; Ross, Donald G.; Botstein, David

    1978-01-01

    A number of independent insertions into bacteriophage λ of the translocatable tetracycline-resistance element Tn10 have been isolated and characterized.—The physical positions and relative orientations of several such insertions were determined. Two independent insertions appear to lie in the same orientation at or very near the same site in the cI gene, and two more lie in opposite orientations at or near the same position in or near the rex gene.—Insertions in or near genes cI, rex, and cIII have been characterized genetically for their effects on expression of nearby genes. Tn10 appears to exert a polar effect on expression of distal genes when it is inserted within an operon, even when expression of that operon is under the influence of λ N-function. In addition, Tn10 insertions in rex appear to influence in some way expression of an "upstream" gene, cI.—Lambda derivatives carrying Tn10 give rise to spontaneously occurring, tetracycline-sensitive deletions at high frequencies. It is likely that formation of these deletions is promoted in some way by the Tn10 element.—Lambda::Tn10 phages carrying a Tn10 element that has undergone several successive cycles of translocation since its first isolation and characterization have been analyzed. The results confirm that Tn10 often retains its physical and functional integrity during many cycles of translocation.—Lambda derivatives carrying Tn10 have been used to generate insertions of Tn10 in the chromosome of Escherichia coli. This process is independent of recA function, and seems to be quite analogous to the translocation of Tn10 in Salmonella typhimurium as studied previously. PMID:365678

  1. Sarcomere length dependent effects on the interaction between cTnC and cTnI in skinned papillary muscle strips.

    PubMed

    Li, King-Lun; Ghashghaee, Nazanin Bohlooli; Solaro, R John; Dong, Wenji

    2016-07-01

    Sarcomere length dependent activation (LDA) of myocardial force development is the cellular basis underlying the Frank-Starling law of the heart, but it is still elusive how the sarcomeres detect the length changes and convert them into altered activation of thin filament. In this study we investigated how the C-domain of cardiac troponin I (cTnI) functionally and structurally responds to the comprehensive effects of the Ca(2+), crossbridge, and sarcomere length of chemically skinned myocardial preparations. Using our in situ technique which allows for simultaneous measurements of time-resolved FRET and mechanical force of the skinned myocardial preparations, we measured changes in the FRET distance between cTnI(167C) and cTnC(89C), labeled with FRET donor and acceptor, respectively, as a function of [Ca(2+)], crossbridge state and sarcomere length of the skinned muscle preparations. Our results show that [Ca(2+)], cross-bridge feedback and sarcomere length have different effects on the structural transition of the C-domain cTnI. In particular, the interplay between crossbridges and sarcomere length has significant impacts on the functional structural change of the C-domain of cTnI in the relaxed state. These novel observations suggest the importance of the C-domain of cTnI and the dynamic and complex interplay between various components of myofilament in the LDA mechanism. PMID:26944554

  2. Transcriptional activity of the transposable element Tn10 in the Salmonella typhimurium ilvGEDA operon.

    PubMed

    Blazey, D L; Burns, R O

    1982-08-01

    Polarity of Tn10 insertion mutations in the Salmonella typhimurium ilvGEDA operon depends on both the location and the orientation of the Tn10 element. One orientation of Tn10 insertions in ilvG and ilvE permits low-level expression of the downstream ilvEDA and ilvDA genes, respectively. Our analysis of Salmonella ilv recombinant plasmids shows that this residual ilv expression must result from Tn10-directed transcription and does not reflect the presence of internal promoters in the ilvGEDA operon, as was previously suggested. The opposite orientation of Tn10 insertion in ilvE prevents ilvDA expression, indicating that only one end of Tn10 is normally active in transcribing adjacent genes. Both orientations of Tn10 insertion in ilvD exert absolute polarity on ilvA expression. Expression of ilvA is known to be dependent on effective translation of ilvD, perhaps reflecting the lack of a ribosome binding site proximal to the ilvA sequence. Therefore, recognition of the ability of Tn10 to promote transcription of contiguous genes in the ilvGEDA operon apparently requires the presence of associated ribosome binding sites. PMID:6289328

  3. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... contract for Big South Fork National Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public... National Park Service Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY... contract for the conduct of certain visitor services within Big South Fork National Recreation...

  4. Architecture of the Tn7 Post-Transposition Complex: an Elaborate Nucleoprotein Structure

    PubMed Central

    Holder, Jason W.; Craig, Nancy L.

    2010-01-01

    Four transposition proteins encoded by the bacterial transposon Tn7, TnsA, TnsB, TnsC, and TnsD, mediate its site- and orientation-specific insertion into the chromosomal site attTn7. To establish which Tns proteins are actually present in the transpososome that executes DNA breakage and joining, we have determined the proteins present in the nucleoprotein product of transposition, the Post-Transposition Complex (PTC) using fluorescently labeled Tns proteins. All four required Tns proteins are present in the PTC in which we also find that the Tn7 ends are paired by protein-protein contacts between Tns proteins bound to the ends. Quantification of the relative amounts of the fluorescent Tns proteins in the PTC indicates that oligomers of TnsA, TnsB, and TnsC mediate Tn7 transposition. High-resolution DNA footprinting of the DNA product of transposition attTn7∷Tn7 revealed that about 350 bp of DNA on the transposon ends and on attTn7 contact the Tns proteins. All seven binding sites for TnsB, the component of the transposase that specifically binds the ends and mediates 3’ end breakage and joining, are occupied in the PTC. However, the protection pattern of the sites closest to the Tn7 ends in the PTC are different from that observed with TnsB alone, likely reflecting the pairing of the ends and their interaction with the target nucleoprotein complex necessary for activation of the breakage and joining steps. We also observe extensive protection of the attTn7 sequences in the PTC and that alternative DNA structures in substrate attTn7 that are imposed by TnsD are maintained in the PTC. PMID:20538004

  5. Suberoylanilide Hydroxamic Acid Restores Estrogen Reduced-cTnI Expression in Neonatal Hearts of Mice.

    PubMed

    Peng, Chang; Luo, Xiaomei; Xing, Qianlu; Sun, Huichao; Huang, Xupei

    2016-10-01

    Diastolic cardiac dysfunction can be caused by abnormality in cTnI expression during cardiogenesis. In this study, we investigated the effects of estrogen on the abnormal expression of cTnI in the hearts of neonatal mice and its potential epigenetic mechanisms. We then evaluated suberoylanilide hydroxamic acid (SAHA), a HDAC inhibitor, as a new target treatment of diastolic cardiac dysfunction. Postnatal day 0.5 C57BL/6 mice were injected with estrogen for 1 week, then the hearts of 7-day-old neonatal mice were retrieved for examination. The activities of HDAC and HAT were assayed by colorimetry, and the interaction of cTnI with HDAC5 in mice hearts were examined using chromatin immunoprecipitation assays. The expression of cTnI was tested by quantitative real-time RT-PCR and Western blot. Estrogen treated groups displayed a significantly increased HDAC activity in the hearts of neonatal mice while HAT activity remained unchanged. Additionally, HDAC5 was higher at the cTnI promoter, as compared to the saline treated control groups. The acetylation of histone H3K9ac on cTnI promoter significantly decreased in the hearts of neonatal mice treated with estrogen, and the expression of cTnI at transcriptional and protein levels also decreased. SAHA was shown to increase the acetylation of histone H3K9ac and upregulate the expression of cTnI. The data demonstrated that SAHA can correct cTnI expression abnormality caused by estrogen through inhibiting the binding of HDAC5 to the promoter of cTnI. J. Cell. Biochem. 117: 2377-2384, 2016. © 2016 Wiley Periodicals, Inc. PMID:27379430

  6. Glycoprotein screening in colorectal cancer based on differentially expressed Tn antigen.

    PubMed

    Wei, Hongyun; Cheng, Zongyong; Ouyang, Chunhui; Zhang, Yu; Hu, Yanyan; Chen, Shuijiao; Wang, Chunlian; Lu, Fanggen; Zhang, Jie; Wang, Yongjun; Liu, Xiaowei

    2016-09-01

    Colorectal cancer (CRC) is one of the most common cancers worldwide, and the identification of new biomarkers for CRC is valuable for its diagnosis and treatment. We aimed to screen differentially expressed glycoproteins (especially O-glycoproteins) and to identify diagnostic or therapeutic candidates for colorectal cancer (CRC) based on different Tn antigen expression levels. Fresh cancer tissues and adjacent healthy tissues were obtained from CRC patients and classified into three groups based on their Tn antigen expression: CRC with negative Tn expression (CRC Tn‑), CRC with positive Tn expression (CRC Tn+) and normal control without Tn expression (NC). Protein extractions were separated and identified by iTRAQ technology. Glycoproteins and O-glycoproteins were selected using UniProt and DAVID. Deep bioinformatic analysis, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KO), was used to annotate this O-glycoprotein interaction network. Subsequently, two O‑glycoproteins were verified by western blotting and immunohistochemistry in either LS174T cells or CRC tissues. We found that 330 differentially expressed proteins were identified by iTRAQ between CRC Tn‑ and NC tissues, 317 between CRC Tn+ and NC tissues, and 316 between CRC Tn‑ and Tn+ tissues. Of the 316 proteins, 55 glycoproteins and 19 O‑glycoproteins were identified and analyzed via deep informatics. Namely, different Tn antigen expression levels in CRC led to differential protein expression patterns, especially for glycoproteins and O‑glycoproteins. Decorin and SORBS1, two representative functional O-glycoproteins, were significantly downregulated in the CRC Tn+ tissues compared with the level in the CRC Tn‑ or NC tissues. Based on this deep bioinformatic analysis, Decorin and SORBS1 are hypothesized to be involved in the TGF‑β and PPAR‑γ signaling pathways, respectively. PMID:27432485

  7. Spectroscopic and ITC study of the conformational change upon Ca{sup 2+}-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle

    SciTech Connect

    Yumoto, Fumiaki; Tanaka, Hiroyuki; Nagata, Koji; Miyauchi, Yumiko; Miyakawa, Takuya; Ojima, Takao; Tanokura, Masaru

    2008-04-25

    Akazara scallop (Chlamys nipponensis akazara) troponin C (TnC) of striated adductor muscle binds only one Ca{sup 2+} ion at the C-terminal EF-hand motif (Site IV), but it works as the Ca{sup 2+}-dependent regulator in adductor muscle contraction. In addition, the scallop troponin (Tn) has been thought to regulate muscle contraction via activating mechanisms that involve the region spanning from the TnC C-lobe (C-lobe) binding site to the inhibitory region of the TnI, and no alternative binding of the TnI C-terminal region to TnC because of no similarity between second TnC-binding regions of vertebrate and the scallop TnIs. To clarify the Ca{sup 2+}-regulatory mechanism of muscle contraction by scallop Tn, we have analyzed the Ca{sup 2+}-binding properties of the complex of TnC C-lobe and TnI peptide, and their interaction using isothermal titration microcalorimetry, nuclear magnetic resonance, circular dichroism, and gel filtration chromatography. The results showed that single Ca{sup 2+}-binding to the Site IV leads to a structural transition not only in Site IV but also Site III through the structural network in the C-lobe of scallop TnC. We therefore assumed that the effect of Ca{sup 2+}-binding must lead to a change in the interaction mode between the C-lobe of TnC and the TnI peptide. The change should be the first event of the transmission of Ca{sup 2+} signal to TnI in Tn ternary complex.

  8. DRAFT LANDSAT DATA MOSAIC: MONTGOMERY COUNTY, TEXAS; HARRIS COUNTY, TEXAS; FORT BEND COUNTY, TEXAS; BRAZORIA COUNTY, TEXAS; GALVESTON COUNTY, TEXAS

    EPA Science Inventory

    This is a draft Landsat Data Mosaic, which contains remote sensing information for Montgomery County, Texas Harris County, Texas Fort Bend County, Texas Brazoria County, Texas Galveston County, and Texas Imagery dates on the following dates: October 6, 1999 and September 29, 200...

  9. Rural County Report. County Level Data Base.

    ERIC Educational Resources Information Center

    Murray, J. Dennis

    This report represents a county-level Pennsylvania data base focused on variables of significance to rural communities. The data includes computations of per capita rates for counties in rural, urban influence, and urban clusters. This report is intended for comparing rural counties to other areas of the state. Three categories are used to present…

  10. International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21▿

    PubMed Central

    Novais, Ângela; Baquero, Fernando; Machado, Elisabete; Cantón, Rafael; Peixe, Luísa; Coque, Teresa M.

    2010-01-01

    TEM-24 remains one of the most widespread TEM-type extended-spectrum β-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-ΔTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly

  11. Alpha Heating and TN Burn in NIF Experiments

    NASA Astrophysics Data System (ADS)

    Cheng, Baolian; Kwan, Thomas; Wang, Yi-Ming; Merrill, Frank; Cerjan, Charlie; Batha, Steven

    2015-11-01

    Sustainable TN burn requires alpha-particle energy deposition in the hot fuel. Recently, we developed an analytic model to estimate the neutron yield generated by the alpha-particle energy deposited in the hot spot, in terms of the measured total neutron yield, the adiabat of the cold fuel and the peak implosion kinetic energy of the pusher. Our alpha heating model has been applied to a number of inertial confinement fusion capsule experiments performed at the National Ignition Facility (NIF). Our model predictions are consistent with the post-shot calibrated code simulations and experimental data. We have also studied the uncertainty and sensitivities of alpha heating on various physics parameters, such as the adiabat of cold fuel, total neutron yield and peak implosion velocity. Our analysis demonstrates that the alpha particle heating was appreciable in only high-foot experiments. Based on our work, we will discuss paths and parameters to reach ignition at NIF (LA-UR-15-25507). This work was performed under the auspices of the U.S. Department of Energy by the Los Alamos National Laboratory under Contract No. W-7405-ENG-36.

  12. Synapsis and catalysis by activated Tn3 resolvase mutants

    PubMed Central

    Olorunniji, Femi J.; He, Jiuya; Wenwieser, Sandra V.C.T.; Boocock, Martin R.; Stark, W. Marshall

    2008-01-01

    The serine recombinase Tn3 resolvase catalyses recombination between two 114 bp res sites, each of which contains binding sites for three resolvase dimers. We have analysed the in vitro properties of resolvase variants with ‘activating’ mutations, which can catalyse recombination at binding site I of res when the rest of res is absent. Site I × site I recombination promoted by these variants can be as fast as res × res recombination promoted by wild-type resolvase. Activated variants have reduced topological selectivity and no longer require the 2–3′ interface between subunits that is essential for wild-type resolvase-mediated recombination. They also promote formation of a stable synapse comprising a resolvase tetramer and two copies of site I. Cleavage of the DNA strands by the activated mutants is slow relative to the rate of synapsis. Stable resolvase tetramers were not detected in the absence of DNA or bound to a single site I. Our results lead us to conclude that the synapse is assembled by sequential binding of resolvase monomers to site I followed by interaction of two site I-dimer complexes. We discuss the implications of our results for the mechanisms of synapsis and regulation in recombination by wild-type resolvase. PMID:19015124

  13. Prediction of microcystis blooms based on TN:TP ratio and lake origin.

    PubMed

    Amano, Yoshimasa; Machida, Motoi; Tatsumoto, Hideki; George, Dennis; Berk, Sharon; Taki, Kazuo

    2008-01-01

    We evaluated the relationship between TN:TP ratio and Microcystis growth via a database that includes worldwide lakes based on four types of lake origin (dammed, tectonic, coastal, and volcanic lakes). We used microcosm and mesocosm for the nutrient elution tests with lake water and four kinds of sediment (nontreated, MgO sprinkling treated, dissolved air flotation [DAF] treated, and combined treated sediment) in order to control TN:TP ratio and to suppress Microcystis growth. Microcystis growth was related to TN:TP ratio, with the maximum value at an optimum TN:TP ratio and the minimum values when the TN:TP ratios reached to 0 or "V. The kurtosis of the distribution curve varied with the type of lake origin; the lowest kurtosis was found in dammed lakes, while the highest was found in volcanic lakes. The lake trophic state could affect the change in the kurtosis, providing much lower kurtosis at eutrophic lakes (dammed lakes) than that at oligotrophic lakes (volcanic lakes). The relationship between TN:TP ratio and Microcystis growth could be explained by the nutrient elution tests under controlled TN:TP ratios through the various sediment treatments. A significant suppression of Microcystis growth of 70% could be achieved when the TN:TP ratios exceeded 21. Lake origin could be regarded as an index including morphological and geographical factors, and controlling the trophic state in lakes. The origin rather than trophic state for lakes could be considered as an important factor of TN:TP influences on Microcystis growth. PMID:18604439

  14. A clinical isolate of transposon Tn5 expressing streptomycin resistance in Escherichia coli.

    PubMed Central

    Genilloud, O; Blázquez, J; Mazodier, P; Moreno, F

    1988-01-01

    The central region of transposon Tn5 carries three antibiotic resistance markers: neo, ble, and str. The str gene codes for a phosphotransferase that inactivates streptomycin. This activity is phenotypically expressed in several gram-negative bacteria but not in Escherichia coli. We identified a Tn5 variant in E. coli clinical isolates that express streptomycin resistance. This transposon carries a 6-base-pair deletion within the str gene, near the 3' end. The same kind of mutation had been previously obtained experimentally from Tn5. Images PMID:2830233

  15. Multicopy integration of mini-Tn7 transposons into selected chromosomal sites of a Salmonella vaccine strain

    PubMed Central

    Roos, Karen; Werner, Esther; Loessner, Holger

    2015-01-01

    Chromosomal integration of expression modules for transgenes is an important aspect for the development of novel Salmonella vectors. Mini-Tn7 transposons have been used for the insertion of one such module into the chromosomal site attTn7, present only once in most Gram-negative bacteria. However, integration of multiple mini-Tn7 copies might be suitable for expression of appropriate amounts of antigen or combination of different modules. Here we demonstrate that integration of a 9.6 kb mini-Tn7 harbouring the luciferase luxCDABE (lux) occurs at the natural attTn7 site and simultaneously other locations of the Salmonella chromosome, which were engineered using λ-Red recombinase to contain one or two additional artificial attTn7 sites (a-attTn7). Multicopy integration even at closely spaced attTn7 sites was unexpected in light of the previously reported distance-dependent Tn7 target immunity. Integration of multiple copies of a mini-Tn7 containing a gfp cassette resulted in increasing green fluorescence of bacteria. Stable consecutive integration of two mini-Tn7 encoding lacZ and lux was achieved by initial transposition of lacZ-mini-Tn7, subsequent chromosomal insertion of a-attTn7 and a second round of transposition with lux-mini-Tn7. Mini-Tn7 thus constitutes a versatile method for multicopy integration of expression cassettes into the chromosome of Salmonella and possibly other bacteria. PMID:25488129

  16. Inhibition of fungi and gram-negative bacteria by bacteriocin BacTN635 produced by Lactobacillus plantarum sp. TN635.

    PubMed

    Smaoui, Slim; Elleuch, Lobna; Bejar, Wacim; Karray-Rebai, Ines; Ayadi, Imen; Jaouadi, Bassem; Mathieu, Florence; Chouayekh, Hichem; Bejar, Samir; Mellouli, Lotfi

    2010-10-01

    The aim of this study was to evaluate 54 lactic acid bacteria (LAB) strains isolated from meat, fermented vegetables and dairy products for their capacity to produce antimicrobial activities against several bacteria and fungi. The strain designed TN635 has been selected for advanced studies. The supernatant culture of this strain inhibits the growth of all tested pathogenic including the four Gram-negative bacteria (Salmonella enterica ATCC43972, Pseudomonas aeruginosa ATCC 49189, Hafnia sp. and Serratia sp.) and the pathogenic fungus Candida tropicalis R2 CIP203. Based on the nucleotide sequence of the 16S rRNA gene of the strain TN635 (1,540 pb accession no FN252881) and the phylogenetic analysis, we propose the assignment of our new isolate bacterium as Lactobacillus plantarum sp. TN635 strain. Its antimicrobial compound was determined as a proteinaceous substance, stable to heat and to treatment with surfactants and organic solvents. Highest antimicrobial activity was found between pH 3 and 11 with an optimum at pH = 7. The BacTN635 was purified to homogeneity by a four-step protocol involving ammonium sulfate precipitation, centrifugal microconcentrators with a 10-kDa membrane cutoff, gel filtration Sephadex G-25, and C18 reverse-phase HPLC. SDS-PAGE analysis of the purified BacTN635, revealed a single band with an estimated molecular mass of approximately 4 kDa. The maximum bacteriocin production (5,000 AU/ml) was recorded after a 16-h incubation in Man, Rogosa, and Sharpe (MRS) medium at 30 degrees C. The mode of action of the partial purified BacTN635 was identified as bactericidal against Listeria ivanovii BUG 496 and as fungistatic against C. tropicalis R2 CIP203. PMID:19888697

  17. Asiago classification of ASASSN-15tn as a Type Ia SN

    NASA Astrophysics Data System (ADS)

    Tomasella, L.; Pastorello, A.; Benetti, S.; Cappellaro, E.; Elias-Rosa, N.; Ochner, P.; Tartaglia, L.; Terreran, G.; Turatto, M.

    2015-12-01

    The Asiago Transient Classification Program (Tomasella et al. 2014, AN, 335, 841) reports the spectroscopic classification of ASASSN-15tn discovered by All Sky Automated Survey for SuperNovae (ASAS-SN) in CGCG 496-51 (Atel#8376).

  18. Tn4563 transposition in Streptomyces coelicolor and its application to isolation of new morphological mutants.

    PubMed Central

    Schauer, A T; Nelson, A D; Daniel, J B

    1991-01-01

    The Tn3-like transposon Tn4556 (and its derivatives Tn4560 and Tn4563) has been used for insertion mapping of genetic loci cloned on plasmids, but it has been difficult to obtain chromosomal insertions, largely because of the lack of a strong selection against transposon donor molecules. In this communication, we report two efficient selection techniques for transposition and their use in the isolation of chromosomal insertion mutations. A number of independent Streptomyces coelicolor morphological mutants (bld and whi) were obtained. Two of the bld mutations were mapped to locations on the chromosome by SCP1-mediated conjugation; at least one mutation, bld-5m1, appears to define a novel locus involved in control of S. coelicolor morphogenesis and antibiotic production. Images PMID:1650343

  19. The Levels and Distribution of TN, TP and TOC in the South China Sea

    NASA Astrophysics Data System (ADS)

    Wang, H.; Han, D.

    2012-04-01

    The marine biogeochemistries of carbon, nitrogen and phosphorous have come under increased scrutiny because of their close involvement in climate change and coastal eutrophication. The South China Sea is unique in that located in a subtropical zone, and therefore represents an important regime for biogeochemical studies. However, to our knowledge, few data are available for total nitrogen (TN), total phosphorous (TP) and total organic carbon (TOC) in South Sea, China. The present study aims to contribute to the knowledge of their status through investigating the level and distribution of TN, TP and TOC in South China Sea. A total of 108 seawater samples of 11 sites in south sea, china were collected during August 29- September 4, 2006. An automated and simultaneous method for determination of TN and TOC was applied to all seawater samples. The combined system allowed simultaneous determination for TOC and TN in the same sample using a single injection and provided low detection limits and excellent linear ranges for both TOC and TN. The risk of contamination has been remarkably reduced due to the minimal sample manipulation and automated analyses. And quantitative analyses of TP in seawater were accomplished by a typical chemical method. Concentration ranges of TN and TP were 0.06-0.67, and 0.003-0.071 mg/L, respectively, as well as that of TOC were 0.23-2.51mg/L. The values of TN and TP showed that the status of nutrition is relatively better in south china sea than other marine areas. Moreover, the upright change trend of TN concentration level as well as TP and TOC according to the experimental results at the total 11 sites are simultaneity studied. The concentration of TN initial increases with the increasing of the depth, later the value becomes almost constant. In contrast, the concentration of TOC reduces with the increasing of the depth, later the value becomes almost unchangeable. Compared with the trend of TN and TOC, that of TP appears relatively stable

  20. A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production.

    PubMed

    Ben Salah, Riadh; Trabelsi, Imen; Ben Mansour, Riadh; Lassoued, Saloua; Chouayekh, Hichem; Bejar, Samir

    2012-08-01

    This study aimed to determine the probiotic potential of 100 strains of Lactic Acid Bacteria (LAB) isolated from different intestinal segments of indigenous poultry in Tunisia. The strains were submitted to a battery of standard tests and criteria commonly used for determining their probiotic properties and attributes. The findings revealed that 19 of the isolates exhibited antimicrobial activity against 4 pathogenic bacteria, and that 4 (TN1, TN8, TN7, and TN13) showed good resistance to pH 3 and 5% bovine bile. Three isolates, namely TN1, TN8, and TN13, showed sensitivity to several antibiotics and were, therefore, selected for further enzymatic activity assays. Two isolates, namely TN1 and TN8, showed high efficacy of adhesion to chicken enterocytes. The cytokines released after stimulation by the two isolates showed high anti-inflammatory profiles, with an increased rate of Interleukin-10 (IL-10) production for the TN8 strain. Showing the highest performance, TN8 was submitted to 16S rRNA gene sequencing, which revealed that the strain was of the species Lactobacillus plantarum. Overall, the findings indicate that the Lactobacilli from poultry intestine has a number of promising properties that make it candidate for application as a probiotic additive in poultry industry. PMID:22634330

  1. Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp.

    PubMed Central

    McHenney, M A; Baltz, R H

    1991-01-01

    Transposon Tn5096 was inserted into a derivative of the temperature-sensitive plasmid pMT660 containing the bacteriophage FP43 pac site. The resulting plasmid, pRHB126, was transduced by FP43 into several Streptomyces species. Tn5096 transposed from pRHB126 into different sites in the genomes of Streptomyces ambofaciens, Streptomyces cinnamonensis, Streptomyces coelicolor A3(2), Streptomyces fradiae, Streptomyces griseofuscus, and Streptomyces thermotolerans. Images PMID:1653214

  2. The role of TF- and Tn-antigens in breast cancer metastasis.

    PubMed

    Kölbl, Alexandra C; Jeschke, Udo; Friese, Klaus; Andergassen, Ulrich

    2016-06-01

    Almost 40 years ago, researchers found out that the Thomsen-Friedenreich (TF) and the Thomsen nouvelle (Tn) antigens could be detected in carcinoma, but not in healthy tissue. A short time after that it became clear that TF and Tn are precursor molecules of the MN-blood group antigens. In normal tissue TF and Tn are coated by glycosyl structures, thereby forming the glycoproteins which are known to account for the MN-blood group, but in malignant tissue these molecules are uncovered.TF, which has an additional Galectin-residue compared to Tn, is correlated with a more favourable prognosis for patients. On the contrary, patients with Tn-bearing tissues have a worse prognosis for overall and progression-free survival. It is known that TF and Tn are involved in the adhesion of tumour cells to the endothelium via a mechanism recruiting Galectin-3 and MUC-1, which is the first step in metastasis formation. Furthermore, it became clear that this pathway can be blocked by a growing number of molecules, thereby creating ways of therapeutical intervention. PMID:26758176

  3. 78 FR 7993 - Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-05

    ... rulemaking (SNPRM) to amend Class D and Class E airspace in the Tri-Cities, TN, area (77 FR 59573). The SNPRM... read as follows: ] Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR... originally proposed in the NPRM of April 28, 2012 (77 FR 21505). Interested parties were invited...

  4. Variation on a theme; an overview of the Tn916/Tn1545 family of mobile genetic elements in the oral and nasopharyngeal streptococci

    PubMed Central

    Santoro, Francesco; Vianna, Morgana E.; Roberts, Adam P.

    2014-01-01

    The oral and nasopharyngeal streptococci are a major part of the normal microbiota in humans. Most human associated streptococci are considered commensals, however, a small number of them are pathogenic, causing a wide range of diseases including oral infections such as dental caries and periodontitis and diseases at other body sites including sinusitis and endocarditis, and in the case of Streptococcus pneumoniae, meningitis. Both phenotypic and sequence based studies have shown that the human associated streptococci from the mouth and nasopharynx harbor a large number of antibiotic resistance genes and these are often located on mobile genetic elements (MGEs) known as conjugative transposons or integrative and conjugative elements of the Tn916/Tn1545 family. These MGEs are responsible for the spread of the resistance genes between streptococci and also between streptococci and other bacteria. In this review we describe the resistances conferred by, and the genetic variations between the many different Tn916-like elements found in recent studies of oral and nasopharyngeal streptococci and show that Tn916-like elements are important mediators of antibiotic resistance genes within this genus. We will also discuss the role of the oral environment and how this is conducive to the transfer of these elements and discuss the contribution of both transformation and conjugation on the transfer and evolution of these elements in different streptococci. PMID:25368607

  5. The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916.

    PubMed Central

    Tribble, G D; Parker, A C; Smith, C J

    1997-01-01

    The Bacteroides mobilizable transposon Tn4555 is a 12.2-kb molecule that encodes resistance to cefoxitin. Conjugal transposition is hypothesized to occur via a circular intermediate and is stimulated by coresident tetracycline resistance elements and low levels of tetracycline. In this work, the ends of the transposon were identified and found to consist of 12-bp imperfect inverted repeats, with an extra base at one end. In the circular form, the ends were separated by a 6-bp "coupling sequence" which was associated with either the left or the right transposon terminus when the transposon was inserted into the chromosome. Tn4555 does not duplicate its target site upon insertion. Using a conjugation-based transposition assay, we showed that the coupling sequence originated from 6 bases of genomic DNA flanking either side of the transposon prior to excision. Tn4555 preferentially transposed into a 589-bp genomic locus containing a 207-bp direct repeat. Integration occurred before or after the repeated sequence, with one integration site between the two repeats. These observations are consistent with a transposition model based on site-specific recombination. In the bacteriophage lambda model for site-specific recombination, the bacteriophage recombines with the Escherichia coli chromosome via a 7-bp "crossover" region. We propose that the coupling sequence of Tn4555 is analogous in function to the crossover region of lambda but that unlike the situation in lambda, recombination occurs between regions of nonhomologous DNA. This ability to recombine into divergent target sites is also a feature of the gram-positive bacterial transposon Tn916. PMID:9098073

  6. Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance.

    PubMed Central

    Sundström, L; Swedberg, G; Sköld, O

    1993-01-01

    Two different enteric plasmids of widely separate origins were observed to carry a new 15.3-kb trimethoprim resistance transposon, Tn5086, also mediating resistance to mercuric ions and to a low level of sulfonamide. The trimethoprim resistance gene characterized from Tn5086 was found to be distinct from those found earlier and was designated type VII. Molecular analysis demonstrated that Tn5086 is closely related to Tn21. The internal part of Tn21 and Tn5086, the element referred to as the integron, was found to be different. First, the integron of Tn5086 contains a 0.62-kb cassette formed by the trimethoprim resistance gene dhfrVII and its immediate surroundings instead of the 0.86-kb aadA1 cassette of Tn21. Second, the integron of Tn5086 lacks a 4.2-kb segment 3' of sulI in Tn21. The dhfrVII gene commences with a UUG codon but was otherwise seen to be markedly related to the cassette genes dhfrI, dhfrV, and dhfrVI. The four related dihydrofolate reductases of 157 amino acids encoded by these genes contain a glutamate instead of the aspartic acid residue found at position 27 of the active center of the chromosomal enzyme from Escherichia coli. PMID:8383666

  7. Expression profile of mucin-associated sialyl-Tn antigen in Chinese patients with different colorectal lesions (adenomas, carcinomas)

    PubMed Central

    Xu, Feng; Fan, Cuizhen; Fan, Shanshan; Liu, Fuquan; Wen, Tao; An, Guangyu; Feng, Guosheng

    2015-01-01

    Background: The sialyl-Tn (sTn) antigen is a mucin-associated carbohydrate antigen expressed by numerous human carcinomas, and is also claimed to be a prognostic factor in colorectal cancer. But the associations between sTn and colorectal cancer remain elusive and controversial. Here, we investigated the expression profile of sTn antigen in a series of human colorectal tissue samples including normal colon, colorectal adenomas, and colorectal carcinomas (CRCs), with an aim to analyzing whether sTn plays a role in the progression and development of Chinese patients with CRCs. Methods: Immunohistochemical staining of sTn antigen was performed in formalin-fixed, paraffin-embedded colonic sections from 4 healthy controls, 44 patients with colorectal adenomas, and 186 patients with primary CRCs. Results: No sTn antigen was detected in normal colonic tissues. There were 41 of 44 patients with colorectal adenomas (93.2%), and 141 of 186 patients with CRCs (75.8%) found to express sTn antigen. The patterns of sTn localization were different in adenomas and carcinomas of colonic tissues. Colorectal adenomas showed predominant supranuclear distribution of sTn antigen, while carcinomas revealed apical membrane, mucin droplet and diffuse cytoplasmic localization. Notably, sTn was significantly associated with the degree of differentiation (P = 0.006) and perineural invasion (P = 0.041) of the tumors, but was independent of age, gender, tumor location, depth of penetration, status of lymph nodes, lymphovascular invasion and TNM stage. Conclusions: These results indicate that sTn may play a role in initiating colorectal carcinogenesis and promoting tumor progression. Determination of sTn expression and localization may assist in evaluating malignant status of colorectal lesions. PMID:26617889

  8. Expression of slow skeletal TnI in adult mouse hearts confers metabolic protection to ischemia

    PubMed Central

    Pound, Kayla M.; Arteaga, Grace M.; Fasano, Mathew; Wilder, Tanganyika; Fischer, Susan K.; Warren, Chad M.; Wende, Adam R.; Farjah, Mariam; Abel, E. Dale; Solaro, R. John; Lewandowski, E. Douglas

    2011-01-01

    Changes in metabolic and myofilament phenotypes coincide in developing hearts. Posttranslational modification of sarcomere proteins influences contractility, affecting the energetic cost of contraction. However, metabolic adaptations to sarcomeric phenotypes are not well understood, particularly during pathophysiological stress. This study explored metabolic adaptations to expression of the fetal, slow skeletal muscle troponin I (ssTnI). Hearts expressing ssTnI exhibited no significant ATP loss during 5 minutes of global ischemia, while non-transgenic littermates (NTG) showed continual ATP loss. At 7 min ischemia TG-ssTnI hearts retained 80±12% of ATP vs. 49±6% in NTG (P<0.05). Hearts expressing ssTnI also had increased AMPK phosphorylation. The mechanism of ATP preservation was augmented glycolysis. Glycolytic end products (lactate and alanine) were 38% higher in TG-ssTnI than NTG at 2 min and 27% higher at 5 min. This additional glycolysis was supported exclusively by exogenous glucose, and not glycogen. Thus, expression of a fetal myofilament protein in adult mouse hearts induced elevated anaerobic ATP production during ischemia via metabolic adaptations consistent with the resistance to hypoxia of fetal hearts. The general findings hold important relevance to both our current understanding of the association between metabolic and contractile phenotypes and the potential for invoking cardioprotective mechanisms against ischemic stress. PMID:21640727

  9. Nodulating Competitiveness of a Nonmotile Tn7 Mutant of Bradyrhizobium japonicum in Nonsterile Soil †

    PubMed Central

    Liu, Ruilong; Tran, Van Mai; Schmidt, E. L.

    1989-01-01

    A nonmotile mutant of Bradyrhizobium japonicum serogroup 127 was generated by Tn7 mutagenesis and matched with the wild type against a common competitor in studies of soybean nodulation in nonsterile soil. The Tn7 mutant was very similar to the wild type in growth rate in culture, soybean lectin-binding ability, flagellar morphology, and nodulating capability, but it had a longer lag phase. Competing strains were distributed uniformly in soil in various ratios and at different population densities prior to planting. Mutant and wild type were equally prevalent in the seedling rhizosphere at about the time of nodule initiation, suggesting that motility conferred no advantage in rhizosphere colonization. Nodulation success of the Tn7 mutant was lower than that of the wild type under all test conditions. Differences were greatest at low soil populations of competitors and much less pronounced at initial populations of 107 g−1. The longer lag phase of the Tn7 mutant may have contributed to its decreased competitiveness, especially at the higher inoculation levels. The antibiotic and motility markers were stable, and the rifampin resistance derived from the parent did not affect adversely the competitiveness of the Tn7 mutant. We found motility to be of limited importance to the competitiveness of a strain in normal nonsterile soil, where the significance, if any, of this ability may be in migration at the immediate root surface in soils sparsely populated with rhizobial symbionts. PMID:16347986

  10. [Variations of annual load of TN and TP in the deep bay watershed, Shenzhen].

    PubMed

    Zhao, Chen-Chen; Zhang, Shi-Yan; Mao, Xian-Zhong

    2014-11-01

    The empirical coefficient of sewage disposal, export coefficient model and mean concentration method were respectively used to estimate variations of annual load TN and TP from Shenzhen and Hong Kong areas in the Deep Bay Watershed from 1986 to 2011. The results showed that, the annual average loads of TN and TP were 10 388.2 t, 10 727.9 t, 10 937.3 t, and 2 694.5 t, 1 929.2 t, 1388.7 t, respectively in the whole watershed during three periods, 80s, 90s and years after 2000. With the rapid development of society, economy and the urbanization, annual pollution loading of TN and TP in Shenzhen area showed an obviously increase, 4373.6 t and 195.9 t, by 261.0% and 64.2% for point source, and 1067.2 t and 151.0 t, by 63.4% and 84.9% for non-point source, respectively. Non-point source with high pollution load was mainly caused by the expanding of land for construction and roads. The contribution ratios of TN and TP from Shenzhen area increased from 42.4% and 27.0% to 85.1% and 75.2%. Annual loads of TN and TP in Hong Kong area decreased 3 028.5 t and 1 031.5 t, by 66.3% and 79.0% reduced. PMID:25639083

  11. TRANSIT--A Software Tool for Himar1 TnSeq Analysis.

    PubMed

    DeJesus, Michael A; Ambadipudi, Chaitra; Baker, Richard; Sassetti, Christopher; Ioerger, Thomas R

    2015-10-01

    TnSeq has become a popular technique for determining the essentiality of genomic regions in bacterial organisms. Several methods have been developed to analyze the wealth of data that has been obtained through TnSeq experiments. We developed a tool for analyzing Himar1 TnSeq data called TRANSIT. TRANSIT provides a graphical interface to three different statistical methods for analyzing TnSeq data. These methods cover a variety of approaches capable of identifying essential genes in individual datasets as well as comparative analysis between conditions. We demonstrate the utility of this software by analyzing TnSeq datasets of M. tuberculosis grown on glycerol and cholesterol. We show that TRANSIT can be used to discover genes which have been previously implicated for growth on these carbon sources. TRANSIT is written in Python, and thus can be run on Windows, OSX and Linux platforms. The source code is distributed under the GNU GPL v3 license and can be obtained from the following GitHub repository: https://github.com/mad-lab/transit. PMID:26447887

  12. Quantitative assessment of Tn antigen in breast tissue micro-arrays using CdSe aqueous quantum dots.

    PubMed

    Au, Giang H T; Mejias, Linette; Swami, Vanlila K; Brooks, Ari D; Shih, Wan Y; Shih, Wei-Heng

    2014-03-01

    In this study, we examined the use of CdSe aqueous quantum dots (AQDs) each conjugated to three streptavidin as a fluorescent label to image Tn antigen expression in various breast tissues via a sandwich staining procedure where the primary monoclonal anti-Tn antibody was bound to the Tn antigen on the tissue, a biotin-labeled secondary antibody was bound to the primary anti-Tn antibody, and finally the streptavidin-conjugated AQDs were bound to the biotin on the secondary antibody. We evaluated the AQD staining of Tn antigen on tissue microarrays consisting of 395 cores from 115 cases including three tumor cores and one normal-tissue core from each breast cancer case and three tumor cores from each benign case. The results indicated AQD-Tn staining was positive in more than 90% of the cells in the cancer cores but not the cells in the normal-tissue cores and the benign tumor cores. As a result, AQD-Tn staining exhibited 95% sensitivity and 90% specificity in differentiating breast cancer against normal breast tissues and benign breast conditions. These results were better than the 90% sensitivity and 80% specificity exhibited by the corresponding horse radish peroxidase (HRP) staining using the same antibodies on the same tissues and those of previous studies that used different fluorescent labels to image Tn antigen. In addition to sensitivity and specificity, the current AQD-Tn staining with a definitive threshold was quantitative. PMID:24411673

  13. 76 FR 53115 - Foreign-Trade Zone 77-Memphis, TN; Application for Temporary/Interim Manufacturing Authority...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-25

    ... Manufacturing Authority; Flextronics Logistics USA, Inc. (Cell Phone/Mobile Handset Kitting); Memphis, TN An... FTZ 77 at the Flextronics Logistics USA, Inc. (Flextronics) facility, located in Memphis,...

  14. Natural transfer of conjugative transposon Tn916 between gram-positive and gram-negative bacteria.

    PubMed

    Bertram, J; Strätz, M; Dürre, P

    1991-01-01

    The conjugative streptococcal transposon Tn916 was found to transfer naturally between a variety of gram-positive and gram-negative eubacteria. Enterococcus faecalis hosting the transposon could serve as a donor for Alcaligenes eutrophus, Citrobacter freundii, and Escherichia coli at frequencies of 10(-6) to 10(-8). No transfer was observed with several phototrophic species. Mating of an E. coli strain carrying Tn916 yielded transconjugants with Bacillus subtilis, Clostridium acetobutylicum, Enterococcus faecalis, and Streptococcus lactis subsp. diacetylactis at frequencies of 10(-4) to 10(-6). Acetobacterium woodii was the only gram-positive organism tested that did not accept the transposon from a gram-negative donor. The results prove the ability of conjugative transposable elements such as Tn916 for natural cross-species gene transfer, thus potentially contributing to bacterial evolution. PMID:1846142

  15. 78 FR 60375 - Norfolk Southern Railway Company-Discontinuance of Service Exemption-in Claiborne County, TN, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-01

    ... adequately protects affected employees, a petition for partial revocation under 49 U.S.C. 10502(d) must be filed. Provided no formal expression of intent to file an offer of financial assistance (OFA)...

  16. In-site interaction evaluation of Tn density by inhibition/competition assays.

    PubMed

    Robles, Ana; Medeiros, Andrea; Berois, Nora; Balter, Henia S; Pauwels, Ernest K; Osinaga, Eduardo

    2010-05-01

    The tumor-associated structure N-acetyl-galactosamine-O-Ser/Thr (Tn antigen), which is overexpressed in various tumor cell types, notably of the breast, ovary and colon, is an interesting determinant that is useful for cancer diagnosis and follow-up. The aim of this research was to study different assay strategies in order to determine the most sensitive system for further application in epitope characterization and binding assessment. The tetrameric isolectin obtained from Vicia villosa seeds (VVLB(4)) shows high affinity for the tumor-associated structure. A monoclonal antibody against VVLB(4), MabVV(34), was generated, and the interaction between MabVV(34) and VVLB(4) was studied by means of binding and inhibition assays. Several synthetic peptides (10 amino acid sequences) designed from the amino acid sequence of VVLB(4) and obtained from trypsin digestion were tested to determine which amino acids were involved in the interaction between MabVV(34) and VVLB(4). The further unraveling of this epitope was investigated by inhibition using designed synthetic peptides as well as mixtures mimicking variable density effect. Under the experimental circumstances, MabVV(34) was able to inhibit the binding of VVLB(4) to Tn. Two of the four peptide sequences assayed showed better inhibition properties. Finally, mixtures containing these selected sequences allowed the evaluation of binding and inhibition as a function of Tn density. We conclude that the present study facilitates the further development of a specific Tn marker and may contribute to the development of Tn-like radiolabelled peptides or Tn-specific radiolabelled fragments providing a highly selective tool for cancer diagnosis and treatment. This strategy may contribute to characterize the new generation of radiopharmaceuticals for diagnosis and therapy based on biomolecules like antibodies, fragments or peptides, whose application is directly guided by their specific molecular recognition. PMID:20447557

  17. Novel Tn4371-ICE like element in Ralstonia pickettii and Genome mining for comparative elements

    PubMed Central

    2009-01-01

    Background Integrative Conjugative Elements (ICEs) are important factors in the plasticity of microbial genomes. An element related to the ICE Tn4371 was discovered during a bioinformatic search of the Ralstonia pickettii 12J genome. This element was analysed and further searches carried out for additional elements. A PCR method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight Ralstonia pickettii and Ralstonia insidiosa strains were analysed for the presence of the element. Results Comparative sequence analysis of bacterial genomes has revealed the presence of a number of uncharacterised Tn4371-like ICEs in the genomes of several β and γ- Proteobacteria. These elements vary in size, GC content, putative function and have a mosaic-like structure of plasmid- and phage-like sequences which is typical of Tn4371-like ICEs. These elements were found after a through search of the GenBank database. The elements, which are found in Ralstonia, Delftia, Acidovorax, Bordetella, Comamonas, Acidovorax, Congregibacter, Shewanella, Pseudomonas Stenotrophomonas, Thioalkalivibrio sp. HL-EbGR7, Polaromonas, Burkholderia and Diaphorobacter sp. share a common scaffold. A PCR method was designed (based on the Tn4371- like element detected in the Ralstonia pickettii 12J genome) to detect and characterise new elements of this type. Conclusion All elements found in this study possess a common scaffold of core genes but contain different accessory genes. A new uniform nomenclature is suggested for ICEs of the Tn4371 family. Two novel Tn4371-like ICE were discovered and characterised, using the novel PCR method described in two different isolates of Ralstonia pickettii from laboratory purified water. PMID:19941653

  18. Hydration of the dTn.dAn x dTn parallel triple helix: a Fourier transform infrared and gravimetric study correlated with molecular dynamics simulations.

    PubMed Central

    Ouali, M; Gousset, H; Geinguenaud, F; Liquier, J; Gabarro-Arpa, J; Le Bret, M; Taillandier, E

    1997-01-01

    We present a comparative analysis of the water organization around the dTn.dAn x dTn triple helix and the Watson-Crick double helix dTn.dAn respectively by means of gravimetric measurements, infrared spectroscopy and molecular dynamics simulations. The hydration per nucleotide determined by gravimetric and spectroscopic methods correlated with the molecular dynamics simulations shows that at high relative humidity (98% RH) the triple helix is less solvated than the duplex (17 +/- 2 water molecules per nucleotide instead of 21 +/-1). The experimental desorption curves are different for both structures and indicate that below 81% RH the triplex becomes more hydrated than the duplex. At this RH the FTIR spectra show the emergence of N-type sugars in the adenosine strand of the triplex. When the third strand is bound in the major groove of the Watson-Crick duplex molecular dynamics simulations show the formation of a spine of water molecules between the two thymidine strands. PMID:9365262

  19. Synergistic template-free synthesis of dsDNA by Thermococcus nautili primase PolpTN2, DNA polymerase PolB, and pTN2 helicase.

    PubMed

    Béguin, Pierre; Gill, Sukhvinder; Charpin, Nicole; Forterre, Patrick

    2015-01-01

    A combination of three enzymes from the hyperthermophilic archaeon Thermococcus nautili, DNA primase PolpTN2, DNA polymerase PolB, and pTN2 DNA helicase, was found to synthesize up to 300-400 ng/µl dsDNA from deoxynucleotide triphosphates in less than 30 min in the absence of added template DNA and oligonucleotide primer. The reaction did not occur below 64 °C. No synthesis was observed if PolpTN2 or PolB were left out; helicase was not essential but accelerated the reaction. The DNA synthesized consisted of highly reiterated palindromic sequences reaching up to more that 10 kb. Sequence analysis of three independent reaction products synthesized at different temperatures showed that the palindromes shared a common pentanucleotide core, suggesting that random nucleic acid fragments were not responsible for priming the reaction. When enzymes were added sequentially, preincubation with primase plus helicase followed by PolB led to a shorter delay before the onset of the reaction as compared to preincubation with PolB plus helicase followed by primase. This suggests that the primase generates seeds that are subsequently amplified and elongated in synergy with PolB by a mechanism involving hairpin formation and slippage synthesis. PMID:25420601

  20. Billion year thermal histories constrained by zircon (U-Th)/He age-eU correlations: Examples from the Laramide and Sevier Provinces of the western U.S.

    NASA Astrophysics Data System (ADS)

    Guenthner, William; Orme, Devon; Reiners, Peter; Laskowski, Andrew

    2016-04-01

    exhumation in this particular basin, we present an "inheritance envelope" approach for interpreting partially reset detrital zircon (U-Th)/He datasets. This approach uses forward modeling of thermal histories, combined with a damage-diffusivity model and a series of pre-depositional He ages to construct "inheritance envelopes". A forward modeled time-temperature path is a permissible thermal history if an inheritance envelope encompasses the observed age variation in a given dataset. For the Oquirrh dataset, a time-temperature path with a maximum burial temperature of ~170 °C and an initial Sevier-belt related exhumation event at 110 Ma successfully captures the large observed age variation. These results demonstrate that our inheritance envelope approach can be used to describe maximum burial temperatures and the timing of initial exhumation in detrital zircons from sedimentary basins.

  1. Tn125-Related Acquisition of blaNDM-Like Genes in Acinetobacter baumannii

    PubMed Central

    Poirel, Laurent; Bonnin, Rémy A.; Boulanger, Anne; Schrenzel, Jacques; Kaase, Martin

    2012-01-01

    A multidrug-resistant Acinetobacter baumannii isolate recovered from a patient hospitalized in Switzerland after a transfer from Serbia produced the NDM-1 carbapenemase. The blaNDM-1 gene was part of a chromosomally located Tn125 composite transposon bracketed by two copies of the same insertion sequence, ISAba125. This transposon was also associated with the acquisition and expression of the blaNDM-2 gene in an A. baumannii isolate in Germany. Tn125 appears to be the main vehicle for dissemination of blaNDM genes in that species. PMID:22143526

  2. Transfer and expression of the tetracycline resistance transposon Tn925 in Acetobacterium woodii.

    PubMed

    Strätz, M; Gottschalk, G; Dürre, P

    1990-03-01

    The Enterococcus faecalis conjugative plasmid pCF10 was used to introduce Tn925 into Acetobacterium woodii by filter mating. Tetracycline resistance was transferred at frequencies of about 10(-6) per donor, but no plasmid DNA was found in the transconjugants. DNA hybridization analyses of HindIII-digested chromosomal DNA demonstrated the insertion of Tn925 at a variety of locations, whereas wild type DNA showed no hybridization at all. The transconjugants were used as donor in mating experiments with tetracycline-sensitive Bacillus subtilis. Transfer of tetracycline resistance was observed at frequencies of 10(-8) per recipient. PMID:2158923

  3. Comparison of Friction Characteristics on TN and VA Mode Alignment Films with Friction Force Microscopy

    NASA Astrophysics Data System (ADS)

    Kwak, Musun; Chung, Hanrok; Kwon, Hyukmin; Kim, Jehyun; Han, Daekyung; Yi, Yoonseon; Lee, Sangmun; Lee, Chulgu; Cha, Sooyoul

    Using frictional force microscopy (FFM), the friction surface characteristics were compared between twisted nematic (TN) mode and vertical alignment (VA) mode alignment films (AFs). The friction asymmetry was detected depending on temperature conditions on TN mode AF, but not on VA mode AF. The difference between two modes was explained by leaning intermolecular repulsion caused by the pre-tilt angle uniformity and the density of side chain. No level difference according to temperature conditions appeared when the pre-tilt angle were measured after liquid crystal (LC) injection.

  4. Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome

    SciTech Connect

    Shoemaker, N.B.; Getty, C.; Gardner, J.F.; Salyers, A.A.

    1986-03-01

    The gene for resistance to erythromycin and clindamycin, which is carried on the conjugative Bacteroides plasmid, pBF4, has been shown previously to be part of an element (Tn4351) that transposes in Escherichia coli. The authors have now introduced Tn4351 into Bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range IncP plasmid R751 (R751::Tn4351) and (ii) pSS-2, a chimeric plasmid which contains 33 kilobases of pBF4 (including Tn4351) cloned into the IncQ plasmid RSF1010 and which is mobilized by R751. When E. coli HB101, carrying either R751::Tn4351 or R751 and pSS-2, was mated with B. uniformis under aerobic conditions, Em/sup r/ transconjugants were detected at a frequency of 10 /sup -6/ to 10/sup -5/ (R751::Tn4351) or 10/sup -8/ to 10/sup -6/ (R751 and pSS-2). In matings involving pSS-2, all Em/sup r/ transconjugants contained simple insertions of Tn4351 in the chromosome, whereas in matings involving R751::Tn4351, about half of the Em/sup r/ transconjugants had R751 cointegrated with Tn4351 in the chromosome. Of the Em/sup r/ transconjugants, 13% were auxotrophs. Bacteroides spp. which had R751 cointegrated with Tn4351 in the chromosome did not transfer R751 or Tn4351 to E. coli HB101 or to isogenic B. uniformis, nor did the integrated R751 mobilize pE5-2, an E. coli-Bacteroides shuttle vector that contains a transfer origin that is recognized by R751.

  5. 78 FR 9988 - Noise Exposure Map Notice Nashville Interntional Airport, Nashville, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-12

    ... Federal Aviation Administration Noise Exposure Map Notice Nashville Interntional Airport, Nashville, TN... Administration (FAA) announces its determination that the Noise Exposure Maps submitted by Metropolitan Nashville.... DATES: Effective Date: The effective date of the FAA's determination on the noise exposure maps...

  6. 75 FR 28302 - American Food and Vending Spring Hill, TN; Notice of Negative Determination Regarding Application...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-20

    ... published in the Federal Register on April 23, 2010 (75 FR 21358). Pursuant to 29 CFR 90.18(c... Employment and Training Administration American Food and Vending Spring Hill, TN; Notice of Negative... Food and Vending, Spring Hill, Tennessee, was based on the findings that the subject firm did...

  7. Lactobacillus plantarum TN8 exhibits protective effects on lipid, hepatic and renal profiles in obese rat.

    PubMed

    Ben Salah, Riadh; Trabelsi, Imen; Hamden, Khaled; Chouayekh, Hichem; Bejar, Samir

    2013-10-01

    This study aimed to first investigate the immuno-modulatory effects of six newly isolated lactic acid bacteria (LAB) on the peripheral blood mononuclear cells (PBMC) of Wistar rats. Except for Lactobacillus plantarum TN8, all the other strains were noted to induce high levels of pro-inflammatory cytokine IL-12 and low levels of anti-inflammatory cytokine IL-10. The strains also generated low ratios of IL-10/IL-12 cytokine. Strain TN8 was, on the other hand, noted to induce an increase in anti-inflammatory IL-10 cytokine secretion rates and a decrease in pro-inflammatory IL-12, IFN-γ and TNF-α cytokine production. The oral administration of TN8 improved the hepatic and urinary functions of obese rats by inducing decreases (P < 0.05) in alanine amino transferase (ALAT), gamma glutamyl transferase (GGT), plasmatic triglycerides, total cholesterol concentrations, creatinine, urea, and body weight when compared to the control group of animals that underwent an increase in aspartate amino transferase (ASAT) and high density lipoprotein (HDL). Overall, the findings indicate that strain TN8 exhibited a number of attractive properties that might open new promising opportunities for the improvement of various parameters related to animal health performance and the avoidance of antibiotics and drugs as promoting factors. PMID:23891961

  8. 75 FR 52364 - Notice of Intent To Repatriate Cultural Items: Memphis Pink Palace Museum, Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... National Park Service Notice of Intent To Repatriate Cultural Items: Memphis Pink Palace Museum, Memphis... repatriate cultural items in the possession of the Memphis Pink Palace Museum, Memphis, TN, that meet the... remains and associated funerary objects from the site. Officials of the Memphis Pink Palace Museum...

  9. Participation of DNA polymerase II in the increased precise excision of Tn10.

    PubMed

    Nagel, Rosa; Chan, Ana

    2003-06-11

    In this work the involvement of polymerase II (Pol II) in the precise excision of Tn10 stimulated by a dnaB252 thermosensitive (Ts) mutant at the permissive temperature, by a uvrD mutant, or by mitomycin C (MMC) or ultraviolet (UV) light treatment, was investigated. A deltapolB::kan mutant showed a significant decrease in the excision of Tn10 induced by the dnaB mutation, or by MMC or UV treatment, indicating the participation of Pol II in this type of deletion process. However, no effect of Pol II was evidenced in the excision of Tn10 stimulated by the uvrD mutation. The effect of the polB mutation on Tn10 precise excision induced by all these treatments was compared to that of mutations in repair-recombination genes recF and recA. The results reveal that the degree of participation of these genes varies depending on the agent that stimulates the deletion event. PMID:12767351

  10. 77 FR 17360 - Proposed Amendment of Class E Airspace; Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of... continues to read as follows: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3... International Airport, TN (Lat. 35 02'33'' N., long. 89 58'36'' W.) ] Olive Branch, MS, Olive Branch...

  11. Insertion of Tn916 in Neisseria meningitidis resulting in loss of group B capsular polysaccharide.

    PubMed

    Stephens, D S; Swartley, J S; Kathariou, S; Morse, S A

    1991-11-01

    We recently found that the 16.4-kb conjugative transposon Tn916 could be introduced into Neisseria meningitidis by transformation and that it appeared to transpose to many different sites in the chromosome of recipient meningococci. In order to identify transposon-induced alterations of specific meningococcal virulence determinants, a library of meningococcal Tetr transformants containing Tn916 was made and screened for those altered in the production of group B capsular polysaccharide. A capsule-defective mutant, M7, was identified by using monoclonal and polyclonal antisera to group B polysaccharide in immunoblot and agar antiserum procedures. Growth of M7 was similar to that of the parent strain. M7 produced no group B capsular polysaccharide by rocket immunoelectrophoresis, and the mutation was stable during laboratory passage. The capsule-defective phenotype was linked to Tetr, as demonstrated by immunoblot and Southern blot analysis of progeny Tetr transformants (transformants of the parent strain obtained with DNA from M7). A capsule-deficient mutant, O8, was identified by using a similar approach. Analysis of the Tn916 insertions in M7 and O8 indicated that a significant portion of the transposon on either side of the tetM determinant had been lost. The ability of Tn916 to generate defined, stable mutations in meningococcal virulence determinants is demonstrated by our study. PMID:1657783

  12. 78 FR 27029 - Modification of Class C Airspace; Nashville International Airport; TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... International Airport, TN, Class C airspace area (78 FR 6257). Interested parties were invited to participate in... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a regulatory evaluation... FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The incorporation by reference in 14 CFR...

  13. 76 FR 39379 - Expansion of Foreign-Trade Zone 78; Nashville, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... inviting public comment has been given in the Federal Register (75 FR 69398, 11/12/10) and the application... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Expansion of Foreign-Trade Zone 78; Nashville, TN Pursuant to its...

  14. 78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    ... Class D and E airspace in the Tri-Cities, TN, area (78 FR 7993). The line defining the exclusion of...; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034; February...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p....

  15. 75 FR 65584 - Proposed Amendment of Class E Airspace; Savannah, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-26

    ... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of..., 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by... action proposes to amend Class E Airspace at Savannah, TN, as the Pinhook Non-Directional Beacon...

  16. IS10/Tn10 transposition efficiently accommodates diverse transposon end configurations.

    PubMed Central

    Chalmers, R M; Kleckner, N

    1996-01-01

    Transposon Tn10 and its component insertion sequence IS10 move by non-replicative transposition. We have studied the array of reaction intermediates and products in a high efficiency in vitro IS10/Tn10 transposition reaction. Synapsis of two transposon ends, followed by cleavage and strand transfer, can occur very efficiently irrespective of the relative locations and orientations of the two ends. The two participating ends can occur in inverted or direct orientation on the same molecule or, most importantly, on two different molecules. This behavior contrasts sharply with that of Mu, in which transposition is strongly biased in favor of inverted repeat synapsis. Mechanistically, the absence of discrimination amongst various end configurations implies that the architecture within the IS10/Tn10 synaptic complex is relatively simple, i.e. lacking any significant intertwining of component DNA strands. Biologically these observations are important because they suggest that the IS10 insertion sequence module has considerable flexibility in the types of DNA rearrangements that it can promote. Most importantly, it now seems highly probable that a single non-replicative IS10 element can promote DNA rearrangements usually attributed to replicative transposition, i.e. adjacent deletions and cointegrates, by utilizing transposon ends on two sister chromosomes. Other events which probably also contribute to the diversity of IS10/Tn10-promoted rearrangements are discussed. Images PMID:8890185

  17. 75 FR 28303 - Setco Automotive, Inc., Paris, TN; Notice of Revised Determination on Reconsideration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-20

    ... automotive clutch products produced by the workers' firm was directly incorporated into. The Department's Notice of determination was published in the Federal Register on April 23, 2010 (FR 75 21358). The... Employment and Training Administration Setco Automotive, Inc., Paris, TN; Notice of Revised Determination...

  18. 75 FR 61696 - Foreign-Trade Zone 148-Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 148--Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon Fiber and Oxidized Polyacrylonitrile Fiber Manufacturing); Rockwood, TN An application has been submitted to the Foreign-Trade...

  19. FATE OF TN5 MUTANTS OF ROOT GROWTH-INHIBITING PSEUDOMONAS SP. IN INTACT SOIL-CORE MICROCOSMS

    EPA Science Inventory

    Transposon Tn5 mutants of a wheat root growth-inhibiting nonfluorescent Pseudomonas sp. were inoculated into intact soil-core microcosms to determine the utility of intact soil cores for evaluating the fate and transport of microorganisms in agricultural ecosystems. ransposon Tn5...

  20. 77 FR 13073 - Designation for the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA Areas

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... hours (7 CFR 1.27(c)). SUPPLEMENTARY INFORMATION: In the September 20, 2011 Federal Register (76 FR...; Memphis, TN; and Sioux City, IA Areas AGENCY: Grain Inspection, Packers and Stockyards Administration... October 20, 2011. In the Lincoln, NE; Memphis, TN; and Sioux City, IA areas, Lincoln, Midsouth, and...

  1. 75 FR 6636 - Foreign-Trade Zone 77-Memphis, TN Application for Subzone Cummins, Inc. (Engine Components...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 77--Memphis, TN Application for Subzone Cummins, Inc. (Engine Components Distribution) Memphis, TN An application has been submitted to the Foreign-Trade Zones Board (the Board) by the City of Memphis,...

  2. Long-Term Variations of TN and TP in Four Typical Chinese Lakes

    NASA Astrophysics Data System (ADS)

    Huang, J.; Xu, Q.; Xi, B.; Wang, X.; Li, W.

    2014-12-01

    Few studies have examined long-term variations in the nutrients that cause lake eutrophication, namely total nitrogen (TN) and total phosphorous (TP). As a result, little is known about the peaks/troughs and trends in TN and TP in lakes known to be vulnerable to eutrophication such as Lake Dongting, Lake Poyang, Lake Chao, and Lake Tai, all of which are located in the Yangtze River basin of China. The objectives of this study were therefore to: 1) detect possible step changes in nutrient time series data; and 2) elucidate TN and TP temporal trends at multiple (annual, seasonal, and monthly) time scales, as influenced by factors such as river-lake connectivity and lake hydrologic conditions (water depth and inflow source). The distribution-free cumulative sum technique and modified Mann-Kendall approach were applied to the long-term nutrient data measured for these four typical lakes and revealed that both TN and TP in Lake Dongting and Lake Poyang exhibited an upward trend at annual, seasonal, and monthly time scales, although they are oligotrophic lakes. The nutrient concentrations in Lake Dongting underwent a step change around 2003, which can be attributed to point and non-point source pollution, overexploitation, and dam construction. The TN values for Lake Chao and Lake Tai experienced step changes in 1999 and 2002, respectively. Before the step-change years, a significant (p-value < 0.05) upward trend in both TN and TP was detected for Lake Chao at the annual scale as well as in summer and May, but for Lake Tai this was manifested at the annual scale only. In contrast, after the step-change years, a significant downward trend was detected for both lakes at the corresponding time scales. As expected, the TN and TP concentrations in Lake Chao and Lake Tai, which are located in the lower basin of the Yangtze River, were noticeably higher than those in Lake Dongting and Lake Poyang, which are located in the middle basin.

  3. 10. Photocopy of photograph showing the three Walker sisters ginning ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Photocopy of photograph showing the three Walker sisters ginning cotton. Misses Hettie, Martha and Louisa are from left to right. The original photograph was taken on May 21, 1936 by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is III-A-HSE-9642. - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

  4. 4. Photocopy of photograph showing the corn crib as it ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Photocopy of photograph showing the corn crib as it looked on May 21, 1936. This structure was also used as a gear shed. Immediately to the right of the shed is Hettie Walker and an unidentified family member or friend. The original photograph was taken by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8091. - Walker Family Farm, Corn Crib, Gatlinburg, Sevier County, TN

  5. Dynamic and Static Water Molecules Complement the TN16 Conformational Heterogeneity inside the Tubulin Cavity.

    PubMed

    Majumdar, Sarmistha; Maiti, Satyabrata; Ghosh Dastidar, Shubhra

    2016-01-19

    TN16 is one of the most promising inhibitors of α, β dimer of tubulin that occupies the cavity in the β-subunit located at the dimeric interface, known as the colchicine binding site. The experimentally determined structure of the complex (Protein Data Bank entry 3HKD) presents the conformation and position of the ligand based on the "best fit", keeping the controversy of other significant binding modes open for further investigation. Computation has already revealed that TN16 experiences fluctuations within the binding pocket, but the insight from that previous report was limited by the shorter windows of sampling and by the approximations on the surrounding environment by implicit solvation. This article reports that in most of the cases straightforward MMGBSA calculations of binding energy revealed a gradual loss of stabilization that was inconsistent with the structural observations, and thus, it indicated the lack of consideration of stabilizing factors with appropriate weightage. Consideration of the structurally packed water molecules in the space between the ligand and receptor successfully eliminated such discrepancies between the structure and stability, serving as the "litmus test" of the importance of explicit consideration of such structurally packed water in the calculations. Such consideration has further evidenced a quasi-degenerate character of the different binding modes of TN16 that has rationalized the observed intrinsic fluctuations of TN16 within the pocket, which is likely to be the most critical insight into its entropy-dominated binding. Quantum mechanical calculations have revealed a relay of electron density from TN16 to the protein via a water molecule in a concerted manner. PMID:26666704

  6. Mechanism underlying insulin uptake in alveolar epithelial cell line RLE-6TN.

    PubMed

    Oda, Keisuke; Yumoto, Ryoko; Nagai, Junya; Katayama, Hirokazu; Takano, Mikihisa

    2011-12-15

    For the development of efficient pulmonary delivery systems for protein and peptide drugs, it is important to understand their transport mechanisms in alveolar epithelial cells. In this study, the uptake mechanism for FITC-insulin in cultured alveolar epithelial cell line RLE-6TN was elucidated. FITC-insulin uptake by RLE-6TN cells was time-dependent, temperature-sensitive, and concentration-dependent. The uptake was inhibited by metabolic inhibitors, cytochalasin D, clathrin-mediated endocytosis inhibitors, and dynasore, an inhibitor of dynamin GTPase. On the other hand, no inhibitory effect was observed with caveolae-mediated endocytosis inhibitors and a macropinocytosis inhibitor. Intracellular FITC-insulin was found to be partly transported to the basal side of the epithelial cell monolayers. In addition, colocalization of FITC-insulin and LysoTracker Red was observed on confocal laser scanning microscopy, indicating that FITC-insulin was partly targeted to lysosomes. In accordance with these findings, SDS-PAGE/fluoroimage analysis showed that intact FITC-insulin in the cells was eliminated with time. The possible receptor involved in FITC-insulin uptake by RLE-6TN cells was examined by using siRNA. Transfection of the cells with megalin or insulin receptor siRNA successfully reduced the corresponding mRNA expression. FITC-insulin uptake decreased on the transfection with insulin receptor siRNA, but not that with megalin siRNA. These results suggest that insulin is taken up through endocytosis in RLE-6TN cells, and after the endocytosis, the intracellular insulin is partly degraded in lysosomes and partly transported to the basal side. Insulin receptor, but not megalin, may be involved at least partly in insulin endocytosis in RLE-6TN cells. PMID:22004610

  7. County Staff or Area Staff?

    ERIC Educational Resources Information Center

    McIntyre, William J.

    1970-01-01

    McIntryre explores the questions of county-based extention and increased specialization in Indiana. He compares the multi-county with individual county systems using variables including clientele's reactions to the two systems. (NL)

  8. 76 FR 50807 - Utah Disaster #UT-00010

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-16

    ... areas have been determined to be adversely affected by the disaster: Primary Counties: Beaver, Box Elder, Cache, Daggett, Duchesne, Emery, Millard, Morgan, Piute, Salt Lake, Sanpete, Sevier, Summit,...

  9. Further characterization of the binding properties of two monoclonal antibodies recognizing human Tn red blood cells.

    PubMed

    Wua, Albert M; Wub, June H; Kuoa, Hsiang-Wei; Herpa, Anthony

    2005-01-01

    The terminal alpha anomeric Ga1NAc residue is an essential sugar for the Tn glycotope, human blood group A determinant, and Forssman antigen. In a previous study [King M.J., Parson S.F., Wu A,M., Jones N., Transfusion 31: 142-149, 1991] we defined two monoclonal antibodies (MoAbs, BRIC66 and BRIC111) reacting with human Tn red blood cells. However, more advanced studies of these two MoAbs were hampered by the lack of availability of Gal/GalNAc related glycotopes. In order to use these antibodies as powerful probes to elucidate structural changes during life processes, we have characterized in detail the combining sites of these two MoAbs using enzyme-linked immunosorbent (ELISA) and inhibition assays with an extended glycan/ligand collection. From the results, it has been established that BRIC66 demonstrated multiple specificities and its reactivity towards glycotopes was defined as: Ga1NAc alpha1-->Ser/Thr (Tn) > or = Ga1NAc alpha1-->3(LFuc alpha1-->2)Gal (Ah) > Ga1NAcalpha1-->3Galbeta1-->4Glc (AL) > Ga1NAalpha1-->3Gal (A) GalNAc alpha1-->3GalNAc > Gal or Glc. Another MoAb, BRIC111, mainly bound Tn-glycophorin. The best ligand for this MoAb was Tn-containing glycopeptides (M.W. < 3.0 x 10(3) Da) from asialo ovine salivary mucin (OSM), which was approximately 70 and 58 times more active than Ga1NAc and monomeric Ga1NAc alpha1-->Ser/Thr (Tn), respectively, suggesting that the active glycotopes present in glycophorin for BRIC111 binding also exist in OSM. The N-acetyl group at carbon-2 and configuration at carbon-2 and carbon-4 of the alpha anomeric Ga1NAc are required for the binding of either MoAb. Identification of these binding properties should aid in the selection of these MoAbs and the conditions required for biological studies and clinical applications. PMID:15864747

  10. Dissection of the Salmonella typhimurium genome by use of a Tn5 derivative carrying rare restriction sites.

    PubMed Central

    Wong, K K; McClelland, M

    1992-01-01

    A polylinker with rare restriction sites was introduced into a mini-Tn5 derivative. These sites include M.XbaI-DpnI (TCTAGATCTAGA), which is rare in most bacterial genomes, SwaI (ATTTAAAT) and PacI (TTAATTAA), which are rare in G+C-rich genomes, NotI (GCGGCCGC) and SfiI (GGCCN5GGCC), which are rare in A+T-rich genomes, and BlnI (CCTAGG), SpeI (ACTAGT), and XbaI (TCTAGA), which are rare in the genomes of many gram-negative bacteria. This Tn5(pfm) (pulsed-field mapping) transposon carries resistance to chloramphenicol and kanamycin to allow selection in a wide variety of background genomes. This Tn5(pfm) was integrated randomly into the Salmonella typhimurium and Serratia marcescens genomes. Integration of the new rare SwaI, PacI, BlnI, SpeI, and XbaI sites was assayed by restriction digestion and pulsed-field gel electrophoresis. Tn5(pfm) constructs could be valuable tools for pulsed-field mapping of gram-negative bacterial genomes by assisting in the production of physical maps and restriction fragment catalogs. For the first applications of a Tn5(pfm), we bisected five of the six largest BlnI fragments in the S. typhimurium genome, bisected the linearized 90-kb pSLT plasmid, and used Tn5(pfm) and Tn10 to trisect the largest BlnI fragment. Images PMID:1317382

  11. Tn-Seq Explorer: A Tool for Analysis of High-Throughput Sequencing Data of Transposon Mutant Libraries

    PubMed Central

    Solaimanpour, Sina; Sarmiento, Felipe; Mrázek, Jan

    2015-01-01

    Tn-seq is a high throughput technique for analysis of transposon mutant libraries. Tn-seq Explorer was developed as a convenient and easy-to-use package of tools for exploration of the Tn-seq data. In a typical application, the user will have obtained a collection of sequence reads adjacent to transposon insertions in a reference genome. The reads are first aligned to the reference genome using one of the tools available for this task. Tn-seq Explorer reads the alignment and the gene annotation, and provides the user with a set of tools to investigate the data and identify possibly essential or advantageous genes as those that contain significantly low counts of transposon insertions. Emphasis is placed on providing flexibility in selecting parameters and methodology most appropriate for each particular dataset. Tn-seq Explorer is written in Java as a menu-driven, stand-alone application. It was tested on Windows, Mac OS, and Linux operating systems. The source code is distributed under the terms of GNU General Public License. The program and the source code are available for download at http://www.cmbl.uga.edu/downloads/programs/Tn_seq_Explorer/ and https://github.com/sina-cb/Tn-seqExplorer. PMID:25938432

  12. Diversity and Evolution of the Tn5801-tet(M)-Like Integrative and Conjugative Elements among Enterococcus, Streptococcus, and Staphylococcus

    PubMed Central

    León-Sampedro, Ricardo; Novais, Carla; Peixe, Luísa; Baquero, Fernando

    2016-01-01

    This work describes the diversity and evolution of Tn5801 among enterococci, staphylococci, and streptococci based on analysis of the 5,073 genomes of these bacterial groups available in gene databases. We also examined 610 isolates of Enterococcus (from 10 countries, 1987 to 2010) for the presence of this and other known CTn-tet(M) elements due to the scarcity of data about Tn5801 among enterococci. Genome location (by ICeu-I–pulsed-field gel electrophoresis [PFGE] hybridization/integration site identification), conjugation and fitness (by standard methods), Tn5801 characterization (by long-PCR mapping/sequencing), and clonality (by PFGE/multilocus sequence typing [MLST]) were studied. Twenty-three Tn5801 variants (17 unpublished) clustered in two groups, designated “A” (25 kb; n = 14; predominant in Staphylococcus aureus) and “B” (20 kb; n = 9; predominant in Streptococcus agalactiae). The percent GC content of the common backbone suggests a streptococcal origin of Tn5801 group B, with further acquisition of a 5-kb fragment that resulted in group A. Deep sequence analysis allowed identification of variants associated with clonal lineages of S. aureus (clonal complex 8 [CC8], sequence type 239 [ST239]), S. agalactiae (CC17), Enterococcus faecium (ST17/ST18), or Enterococcus faecalis (ST8), local variants, or variants located in different species and geographical areas. All Tn5801 elements were chromosomally located upstream of the guaA gene, which serves as an integration hot spot. Transferability was demonstrated only for Tn5801 type B among E. faecalis clonal backgrounds, which eventually harbored another Tn5801 copy. The study documents early acquisition of Tn5801 by Enterococcus, Staphylococcus, and Streptococcus. Clonal waves of these pathogens seem to have contributed to the geographical spread and local evolution of the transposon. Horizontal transfer, also demonstrated, could explain the variability observed, with the isolates often containing

  13. Diversity and Evolution of the Tn5801-tet(M)-Like Integrative and Conjugative Elements among Enterococcus, Streptococcus, and Staphylococcus.

    PubMed

    León-Sampedro, Ricardo; Novais, Carla; Peixe, Luísa; Baquero, Fernando; Coque, Teresa M

    2016-03-01

    This work describes the diversity and evolution of Tn5801 among enterococci, staphylococci, and streptococci based on analysis of the 5,073 genomes of these bacterial groups available in gene databases. We also examined 610 isolates of Enterococcus (from 10 countries, 1987 to 2010) for the presence of this and other known CTn-tet(M) elements due to the scarcity of data about Tn5801 among enterococci. Genome location (by ICeu-I-pulsed-field gel electrophoresis [PFGE] hybridization/integration site identification), conjugation and fitness (by standard methods), Tn5801 characterization (by long-PCR mapping/sequencing), and clonality (by PFGE/multilocus sequence typing [MLST]) were studied. Twenty-three Tn5801 variants (17 unpublished) clustered in two groups, designated "A" (25 kb; n = 14; predominant in Staphylococcus aureus) and "B" (20 kb; n = 9; predominant in Streptococcus agalactiae). The percent GC content of the common backbone suggests a streptococcal origin of Tn5801 group B, with further acquisition of a 5-kb fragment that resulted in group A. Deep sequence analysis allowed identification of variants associated with clonal lineages of S. aureus (clonal complex 8 [CC8], sequence type 239 [ST239]), S. agalactiae (CC17), Enterococcus faecium (ST17/ST18), or Enterococcus faecalis (ST8), local variants, or variants located in different species and geographical areas. All Tn5801 elements were chromosomally located upstream of the guaA gene, which serves as an integration hot spot. Transferability was demonstrated only for Tn5801 type B among E. faecalis clonal backgrounds, which eventually harbored another Tn5801 copy. The study documents early acquisition of Tn5801 by Enterococcus, Staphylococcus, and Streptococcus. Clonal waves of these pathogens seem to have contributed to the geographical spread and local evolution of the transposon. Horizontal transfer, also demonstrated, could explain the variability observed, with the isolates often containing sequences of

  14. An efficient (t,n) threshold quantum secret sharing without entanglement

    NASA Astrophysics Data System (ADS)

    Qin, Huawang; Dai, Yuewei

    2016-04-01

    An efficient (t,n) threshold quantum secret sharing (QSS) scheme is proposed. In our scheme, the Hash function is used to check the eavesdropping, and no particles need to be published. So the utilization efficiency of the particles is real 100%. No entanglement is used in our scheme. The dealer uses the single particles to encode the secret information, and the participants get the secret through measuring the single particles. Compared to the existing schemes, our scheme is simpler and more efficient.

  15. ROLE OF NA+ IN TRANSPORT OF HG2+ AND INDUCTION OF THE TN21 "MER" OPERON

    EPA Science Inventory

    The effects of sodium ions on the uptake of Hg 2 + and induction of the TN21 mer operon were studied using E. coli HMS174 harboring the reporter plasmids pRB28 and pOS14. lasmid pRB28 carries merRT' and pOS14 carries merRTPC of the mer operon, both cloned upstream of a promoterle...

  16. Design analysis report for the TN-WHC cask and transportation system

    SciTech Connect

    Brisbin, S.A., Fluor Daniel Hanford

    1997-02-13

    This document presents the evaluation of the Spent Nuclear Fuel Cask and Transportation System. The system design was developed by Transnuclear, Inc. and its team members NAC International, Nelson Manufacturing, Precision Components Corporation, and Numatec, Inc. The cask is designated the TN-WHC cask. This report describes the design features and presents preliminary analyses performed to size critical dimensions of the system while meeting the requirements of the performance specification.

  17. Tn5 insertion mutants of Pseudomonas aeruginosa deficient in surface expression of ferripyochelin-binding protein

    SciTech Connect

    Sokol, P.A.

    1987-07-01

    Transposon (Tn5) insertion mutants were isolated in Pseudomonas aeruginosa PAO. These mutants were screened for expression of the ferripyochelin-binding protein with monoclonal antibody in a whole-cell immunoblot assay. Fourteen mutants were identified which did not express ferripyochelin-binding protein on the cell surface. These mutants did not take up /sup 59/Fe-labeled pyochelin and grew slowly in the presence of iron chelators.

  18. Effects of supplementation with L. plantarum TN8 encapsulated in alginate-chitosan in broiler chickens.

    PubMed

    Trabelsi, Imen; Ktari, Naourez; Ben Slima, Sirine; Bouchaala, Kamel; Ben Salah, Riadh

    2016-08-01

    This study was undertaken to investigate the effects of supplementation of probiotic strain Lactobacillus plantarum TN8 encapsulated in sodium alginate-chitosan or a commercial blend of essential oils on total cholesterol, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) and growth performance of broiler chickens. The results showed that the broiler chickens supplemented with encapsulated L. plantarum TN8 or essential oil has a higher growth than the control group. After 35days, the weight means were 1860 and 1880g respectively in dietary supplementation with probiotic or essential oil, while they are 1800g in the control group. The evolution of the feed consumption and feed conversion per week showed that the supplementation of encapsulated TN8 strain or essential oil in broiler chickens food has a positive influence on their appetite. Similarly, supplementation of the feed with this encapsulated strain significantly reduced the rate of cholesterol (HDL and LDL) as well as the contents of triglycerides in broiler chickens. Through our study, it appears that the use of the probiotic supplementation or essential oil to broilers were found to be better than the control group of chickens, resulting in a significant economic impact and promoting effect on health. PMID:27181580

  19. Retrieving TN and TP Concentration of Urban River From High Resolution IKONOS Multispectral Imagery

    NASA Astrophysics Data System (ADS)

    Zhang, Y.; Liu, J.; Zhang, L.; Song, X.

    2014-12-01

    Total nitrogen (TN) and Total phosphorus (TP) are widely known as two important indexes to measure China urban rivers, and the technique of remote sensing plays an important role in quantitatively monitoring the dynamic change and timely grasping the status of urban rivers. Taking Wen-rui Tang River as examples, this paper develops both multiple regressions (MR) model and artificial neural networks (ANN) model to estimate TN and TP concentration from high resolution IKONOS image data and in situ water samples collected concurrently with satellite overpass. By analyzing determination coefficients (R2) and relative root mean square error (RMSE), it is found that the measured and estimated values of both MR and ANN models are in good agreement (R2>0.85 and RMSE<2.50), and the estimated accuracy using ANN model is better (R2>0.86 and RMSE<0.89). The results also present the potential of high resolution IKONOS multispectral imagery to apply to urban rivers. The spatial distribution maps of TP and TN concentration generated by ANN model present apparent spatial variations and inform the decision makers of water quality variations in Wen-rui Tang River. The approach developed in this study proves to be effective and has the potential to be applied over urban rivers for water quality monitoring.

  20. Silicon nanowire biosensors for detection of cardiac troponin I (cTnI) with high sensitivity.

    PubMed

    Kim, Kihyun; Park, Chanoh; Kwon, Donghoon; Kim, Donghoon; Meyyappan, M; Jeon, Sangmin; Lee, Jeong-Soo

    2016-03-15

    We have demonstrated highly sensitive and label-free detection of cardiac troponin I (cTnI), a biomarker for diagnosis of acute myocardial infarction, using silicon nanowire field-effect transistors. A honeycomb-like structure is utilized for nanowire configuration to offer improved electrical performance and increased sensing area. The fabricated devices show n-type behavior with a relatively high ON-OFF current ratio, small sub-threshold swing and low gate leakage current. Monoclonal antibodies for cTnI were covalently immobilized on the nanowire surface and the attachment of antibodies is clearly visualized by atomic force microscope. The sensitivity with various concentrations of buffer solution was also investigated in order to determine the optimal buffer condition. The devices exhibit highest sensitivity under buffer solutions with low ion concentration. In addition, the detection limit of the sensor is as low as ~5 pg/mL, the lowest reported in the literature to date and nearly an order of magnitude smaller than the suggested threshold limit. The fabricated devices demonstrate a good selectivity for detecting cTnI. PMID:26496224

  1. Comprehensive identification of virulence factors required for respiratory melioidosis using Tn-seq mutagenesis

    PubMed Central

    Gutierrez, Maria G.; Yoder-Himes, Deborah R.; Warawa, Jonathan M.

    2015-01-01

    Respiratory melioidosis is a disease presentation of the biodefense pathogen, Burkholderia pseudomallei, which is frequently associated with a lethal septicemic spread of the bacteria. We have recently developed an improved respiratory melioidosis model to study the pathogenesis of Burkholderia pseudomallei in the lung (intubation-mediated intratracheal [IMIT] inoculation), which more closely models descriptions of human melioidosis, including prominent septicemic spread from the lung and reduced involvement of the upper respiratory tract. We previously demonstrated that the Type 3 Secretion System cluster 3 (T3SS3) is a critical virulence determinant for B. pseudomallei when delivered directly into the lung. We decided to comprehensively identify all virulence determinants required for respiratory melioidosis using the Tn-seq phenotypic screen, as well as to investigate which virulence determinants are required for dissemination to the liver and spleen. While previous studies have used Tn-seq to identify essential genes for in vitro cultured B. pseudomallei, this represents the first study to use Tn-seq to identify genes required for in vivo fitness. Consistent with our previous findings, we identified T3SS3 as the largest genetic cluster required for fitness in the lung. Furthermore, we identified capsular polysaccharide and Type 6 Secretion System cluster 5 (T6SS5) as the two additional major genetic clusters facilitating respiratory melioidosis. Importantly, Tn-seq did not identify additional, novel large genetic systems supporting respiratory melioidosis, although these studies identified additional small gene clusters that may also play crucial roles in lung fitness. Interestingly, other previously identified virulence determinants do not appear to be required for lung fitness, such as lipopolysaccharide. The role of T3SS3, capsule, and T6SS5 in lung fitness was validated by competition studies, but only T3SS3 was found to be important for respiratory

  2. 76 FR 63281 - Foreign-Trade Zone 78-Nashville, TN, Application for Subzone, Hemlock Semiconductor, L.L.C...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... Semiconductor, L.L.C. (Polysilicon); Clarksville, TN An application has been submitted to the Foreign-Trade... Semiconductor, L.L.C. (HSLLC), located in Clarksville, Tennessee. The application was submitted pursuant to...

  3. Ca2+-regulatory function of the inhibitory peptide region of cardiac troponin I is aided by the C-terminus of cardiac troponin T: Effects of familial hypertrophic cardiomyopathy mutations cTnI R145G and cTnT R278C, alone and in combination, on filament sliding

    PubMed Central

    Brunet, Nicolas M.; Chase, P. Bryant; Mihajlović, Goran; Schoffstall, Brenda

    2014-01-01

    Investigations of cardiomyopathy mutations in Ca2+ regulatory proteins troponin and tropomyosin provide crucial information about cardiac disease mechanisms, and also provide insights into functional domains in the affected polypeptides. Hypertrophic cardiomyopathy-associated mutations TnI R145G, located within the inhibitory peptide (Ip) of human cardiac troponin I (hcTnI), and TnT R278C, located immediately C-terminal to the IT arm in human cardiac troponin T (hcTnT), share some remarkable features: structurally, biochemically, and pathologically. Using bioinformatics, we find compelling evidence that TnI and TnT, and more specifically the affected regions of hcTnI and hcTnT, may be related not just structurally but also evolutionarily. To test for functional interactions of these mutations on Ca2+-regulation, we generated and characterized Tn complexes containing either mutation alone, or both mutations simultaneously. The most important results from in vitro motility assays (varying [Ca2+], temperature or HMM density) show that the TnT mutant “rescued” some deleterious effects of the TnI mutant at high Ca2+, but exacerbated the loss of function, i.e., switching off the actomyosin interaction, at low Ca2+. Taken together, our experimental results suggest that the C-terminus of cTnT aids Ca2+-regulatory function of cTnI Ip within the troponin complex. PMID:24418317

  4. An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.

    PubMed

    Glassing, Angela; Lewis, Thomas A

    2015-11-01

    An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio. PMID:26341612

  5. Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA.

    PubMed Central

    Gamas, P; Craig, N L

    1992-01-01

    The bacterial transposon Tn7 encodes five transposition genes tnsABCDE. We report a simple and rapid procedure for the purification of TnsC protein. We show that purified TnsC is active in and required for Tn7 transposition in a cell-free recombination system. This finding demonstrates that TnsC participates directly in Tn7 transposition and explains the requirement for tnsC function in Tn7 transposition. We have found that TnsC binds adenine nucleotides and is thus a likely site of action of the essential ATP cofactor in Tn7 transposition. We also report that TnsC binds non-specifically to DNA in the presence of ATP or the generally non-hydrolyzable analogues AMP-PNP and ATP-gamma-S, and that TnsC displays little affinity for DNA in the presence of ADP. We speculate that TnsC plays a central role in the selection of target DNA during Tn7 transposition. Images PMID:1317955

  6. The 2011 eruption of Grímsvötn volcano, Iceland

    NASA Astrophysics Data System (ADS)

    Lynch, Rebecca; Thordarson, Thorvaldur

    2015-04-01

    The 2011 eruption of Grímsvötn volcano, Iceland, was much more explosive than previous eruptions, specifically its 2004 eruption. This research examines the degassing processes of the 2011 eruption, through density and vesicule analyses, to help uncover the reasons for the more vigorous eruption. Over 1200 collected tephra samples from the 2011 sequences are measured for density and vesicularity. Several samples are chosen to be representative of eruptive phases; samples from the beginning of the eruption, the mid-eruption and the end phases are chosen. These pumice samples are impregnated with epoxy and made into plugs for use in a Scanning Electron Microscope with which, a nested image approach is taken to image the vesicules of the samples at different magnifications. Each backscatter image is converted to binary and corrected using GIMP. Using ImageJ software, quantitative vesicularity analysis of the images is performed and results are converted to volume. The density, quantitative vesicularity, and volume results are assessed for patterns and the processes of the magma during the ascent in the conduit and eruptive phases are inferred. The objective of this research is to use the microscopic vesicularity analyses of the eruptive products to theorize the larger scale magmatic and degassing processes and to understand why the 2011 Grímsvötn eruption was uncharacteristically explosive. Currently, the results are being examined and have not been included in this abstract, however the research will be finalized in time for presentation at the EGU 2015 conference. Keywords: Grímsvötn volcano, quantitative vesicularity analysis, bubble size distribution, volcanic degassing, conduit processes

  7. 75 FR 52367 - Notice of Inventory Completion: Memphis Pink Palace Museum, Memphis, TN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... Togo site (3CS24), Cross County, AR, during amateur excavations. The human remains were donated to the... amateur excavations. The human remains were donated to the museum in 1972 (Accn. 1972.28.1-5). No...

  8. Cardiac troponin I but not cardiac troponin T adheres to polysulfone dialyser membranes in an in vitro haemodialysis model: explanation for lower serum cTnI concentrations following dialysis

    PubMed Central

    Gaze, David C; Collinson, Paul O

    2014-01-01

    Background Elevated serum cardiac troponin T (cTnT) and I (cTnI) can occur in patients with chronic kidney disease. Differences in cTn concentrations between cTnT and cTnI have been reported but the mechanism of such discrepancy has not been investigated. This study investigates the clearance of cTn with the aid of an in vitro model of haemodialysis (HD). Methods Serum was obtained before and after a single session of dialysis from 53 patients receiving HD and assayed for cTnT and cTnI. An in vitro model of the dialysis process was used to investigate the mechanism of clearance of cTn during HD. Results Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD. Using an in vitro model of dialysis, it was demonstrated that cTnI is not dialysed from the vascular compartment but adheres to the dialyser membrane. Conclusions The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis. The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar. PMID:25332816

  9. Metabolite Profiling Reveals Abiotic Stress Tolerance in Tn5 Mutant of Pseudomonas putida

    PubMed Central

    Chaudhry, Vasvi; Bhatia, Anil; Bharti, Santosh Kumar; Mishra, Shashank Kumar; Chauhan, Puneet Singh; Mishra, Aradhana; Sidhu, Om Prakash; Nautiyal, Chandra Shekhar

    2015-01-01

    Pseudomonas is an efficient plant growth–promoting rhizobacteria (PGPR); however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5) mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC) and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE) and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS) pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA) on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic stress

  10. Dissemination of transposon Tn6001 in carbapenem-non-susceptible and extensively drug-resistant Pseudomonas aeruginosa in Taiwan

    PubMed Central

    Tseng, Sung-Pin; Tsai, Jui-Chang; Teng, Lee-Jene; Hsueh, Po-Ren

    2009-01-01

    Objectives To investigate the prevalence of metallo-β-lactamases (MBLs) and Tn6001 in carbapenem-non-susceptible Pseudomonas aeruginosa (CNSPA). The CNSPA included extensively drug-resistant P. aeruginosa (XDRPA) and non-XDRPA isolates in Taiwan. Methods A total of 308 CNSPA isolates collected at a medical centre from 2000 to 2005 and 26 XDRPA collected from six medical centres in different regions of Taiwan in 2003 were included. MBL genes and Tn6001 were detected by PCR. Clonal relatedness was determined by PFGE. Results Of the 308 CNSPA isolates, 30 (10%) were XDRPA, including 27 (9%) colistin-only-susceptible (COS) and 3 (1%) colistin-only-intermediate (COI) P. aeruginosa. blaVIM-3 was found in 16 (53%) isolates of the XDRPA (n = 30), whereas only 72 (26%) of the non-XDRPA (n = 278) carried the gene. In450 was higher in COS P. aeruginosa (12/27; 44%) than in non-XDRPA isolates (53/278; 19%). Tn6001 was highest in COS P. aeruginosa (11/27; 41%), followed by COI P. aeruginosa (1/3; 33%), and lowest in non-XDRPA (46/278; 17%). Of 26 XDRPA from six medical centres, higher prevalences of blaVIM-3 (16/26; 62%), In450 (16/26; 62%) and Tn6001 (12/26; 46%) were found. Genotyping by PFGE revealed 60 pulsotypes. Hybridization of a blaVIM-3-specific probe following PFGE suggested that the mobile element Tn6001 might have transferred horizontally. Conclusions Tn6001 and In450 play an important role in the dissemination of CNSPA and XDRPA. The prevalence of these genetic constituents was higher in XDRPA than in non-XDRPA isolates, suggesting that the mobile element Tn6001 might have transferred horizontally. PMID:19773253