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1

Fish Lake, Sevier County, Utah.  

National Technical Information Service (NTIS)

Annual total phosphorus and total nitrogen loadings to the lake were estimated and subdivided according to either point or non-point source origin. An assessment of the lake's trophic condition and limiting nutrient is also provided. All data collected by...

1977-01-01

2

Extensional collapse along the Sevier Desert reflection, northern Sevier Desert basin, western United States  

NASA Astrophysics Data System (ADS)

Newly released and previously published seismic reflection data from the northern Sevier Desert basin provide a complete seismic transect between the tilted western margin of the basin and the eastern breakaway zone. When tied to well and surface age data, the transect delineates a continuum of extensional fault and basin fill geometries that developed between late Oligocene and Pleistocene time across the basin. A minimum of 18 km of top-to-the-west normal displacement is estimated across the Sevier Desert from only the most conspicuous growth geometries and offsets across listric normal faults that sole downward into the Sevier Desert reflection (SDR). The SDR clearly marks a normal fault zone beneath the entire basin, where stratal truncations are imaged for 50% of the 39 km length of the reflection east of the Cricket Mountains block. Restoration of extensional displacement along this entire 39 km fault length is necessary to reconstruct the pre-Oligocene configuration and erosion level of Sevier thrust sheets across the Sevier Desert area. The SDR normal fault zone underlies the former topographic crest of the Sevier orogenic belt, where it accommodated extensional collapse after cessation of regional contractile tectonism.

Coogan, James C.; Decelles, Peter G.

1996-10-01

3

77 FR 17408 - Foreign-Trade Zone 204-Tri-Cities Area, TN/VA; Application for Reorganization Under Alternative...  

Federal Register 2010, 2011, 2012, 2013

...Tennessee Business Park, intersection of TN 357 and Highway 75, Kingsport, Sullivan County, TN; Site 4 (129 acres, expires 6...Holston Business and Technology Park, 4509 West Stone Drive, Kingsport, Hawkins County, TN; Site 9 (134 acres, expires...

2012-03-26

4

75 FR 57997 - Tennessee Disaster # TN-00043  

Federal Register 2010, 2011, 2012, 2013

...Declaration 12322 and 12323] Tennessee Disaster TN-00043...Assistance Only for the State of Tennessee (FEMA- 1937-DR...the disaster: Primary Counties: Clay, Cocke, Hardin, Jackson, Macon, Overton, Pickett, Putnam,...

2010-09-23

5

Ground-water hydrology of the Sevier Desert, Utah  

USGS Publications Warehouse

The Sevier Desert, as used in this report, comprises the main part of the Sevier Desert, the Tintic Valley, and the southeastern part of the Old River Bed. It covers an area of about 3,000 square miles and occupies a large basin in the eastern part of the Basin and Range physiographic province. Large alluvial fans extend from the mountain fronts into the basin where they interfinger with eolian and lacustrine deposits and with fluvial deposits of the Sevier River. These unconsolidated deposits form a multiaquifer artesian system that is more than 1,000 feet thick and that extends from near the area of main recharge along the east side of the basin to Sevier Lake. Most of the recharge to the ground-water reservoir results from water entering alluvial fans as percolation from streams, irrigation ditches, and irrigated fields. Another important source may be water in the limestone, quartzite, and other consolidated rocks in the mountains that border the basin. Leakage from the Central Utah Canal is a major source of recharge to the water-table aquifer. Flowing wells are common in the central lowland part of the Sevier Desert, but as a result of below-normal precipitation and an increase in withdrawals from wells during 1950-64, the area of flowing wells has decreased. The quantity of ground water being wasted from flowing wells is not more than a few hundred acre-feet a year. The amount of water discharged by withdrawal from wells has increased nearly 15 times since 1950 (from 2,000 acre-feet in 1950 to 30,000 acre-feet in 1964). As a result of this increasing withdrawal, the water levels in observation wells have declined 4 feet in areas of small withdrawals to more than 7 feet near centers of pumping for public supplies and irrigation. An estimated 135,000-175,000 acre-feet of ground water is consumed by evapotranspiration each year in the 440,000 acres of desert that mainly support phreatophytes. This rate of discharge has changed little since 1950. The consumptive waste of ground water by undesirable phreatophytes, principally saltcedar and pickleweed, was not a serious problem in 1964 but could become a serious problem in the near future if saltcedar is permitted to spread. Water levels in wells changed little during 1935-40. During 1941-50, however, water levels rose in response to the general above-normal precipitation during 1939-47. During 1950-64 water levels declined, partly in response to below-normal precipitation and partly in response to an increase in pumping from irrigation wells. Although the period 1961-63 was one of above-normal precipitation, water levels continued the overall decline that was started in 1950. The decline, therefore, probably is due .to increased pumping. The amount of water that could be obtained from storage if the piezometric surface in the artesian aquifer were lowered 20 feet is estimated to be 120,006 acre-feet. The specific capacities of wells used for irrigation and public supply range from 5 to 215 gallons per minute per foot of drawdown. Specific capacities generally decrease with increasing distances away from the edge of the basin.

Mower, R.W.; Feltis, R.D.

1968-01-01

6

OBVERSE VIEW OF TN24911, LOOKING BACK FROM THE NORTHEAST ROOM ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

OBVERSE VIEW OF TN-249-11, LOOKING BACK FROM THE NORTHEAST ROOM TO THE STAIR HALL. THE DOOR TO THE NORTH (DINING) ROOM LIES AS LEFT - Hilderbrand House, 4571 Airways Boulevard, Memphis, Shelby County, TN

7

New data on the late Pleistocene history of lake fluctuations in the Sevier Desert, Utah  

NASA Astrophysics Data System (ADS)

The chronology of lake-level changes during the late Pleistocene in the Lake Bonneville basin, Utah, is currently understood in a general way. Because the regressive phase of the lake occurred during a critical time in the history of the basin (climate was changing rapidly, humans were moving into suitable habitats that were being made available by the decline of the large lake), it is important to improve the dating of paleohydrologic and paleoclimatic events. We have initiated field and laboratory studies in a part of the Sevier Desert that was studied at a reconnaissance scale 25 to 30 years ago. Our work consists of describing critical stratigraphic sections in natural exposures along the Sevier River and in a core of lake and fluvial sediments, and in dating suitable materials from these sections using radiocarbon and optically stimulated luminescence (OSL). The Sevier basin was integrated with the rest of Lake Bonneville during the time when its highest shorelines were being formed, including during the long period of development of the Provo shoreline (~17.6-14.6 cal ka). The lake regressed rapidly from the Provo shoreline (~1465 m) to the altitude of the divide between the Sevier basin and the Great Salt Lake (GSL) basin (~1390 m), then split into two lakes. In the GSL basin the lake continued to decline as a closed-basin lake, and the lake in the shallow Sevier basin overflowed into it along a large river (the ancestral Sevier River). An extensive wetland, fed by runoff from the Sevier and Beaver Rivers, and by groundwater discharge, developed over much of the Sevier Desert floor during the early Holocene. We have measured new OSL ages that compare favorably with previously obtained radiocarbon ages for the same stratigraphic units. Part of our work is aimed at evaluating the hypothesis that rapid draw-downs in lake level would cause increased groundwater discharge to the lake, and therefore a potential radiocarbon reservoir effect that would have been spatially and temporally heterogeneous.

Oviatt, C. G.; Spencer, J. Q.; Fan, Y.; Leggett, A.

2010-12-01

8

Influence of preexisting tectonic trends on geometries of Sevier orogenic belt and its foreland in Utah  

Microsoft Academic Search

The tectonic style of the late Mesozoic Sevier orogenic belt in Utah was greatly affected by preexisting structural trends that date from the late Precambrian rifting and fragmentation of the North American continent. The late Precambrian cratonic margin (Cordilleran hinge line) was marked by a system of prominent faults including the north-south-trending ancestral Wasatch and ancient Ephraim faults and the

F. Picha; R. I. Gibson

1985-01-01

9

26. Newfound Gap Road, North Carolina side, with Oconaluftee River. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. Newfound Gap Road, North Carolina side, with Oconaluftee River. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

10

15. Newfound Gap parking area with construction scar looking W. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. Newfound Gap parking area with construction scar looking W. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

11

28. Stream view of revetment wall on Newfound Gap Road ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

28. Stream view of revetment wall on Newfound Gap Road looking S. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

12

25. Newfound Gap Road, pullout after switchback, North Carolina side. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. Newfound Gap Road, pullout after switchback, North Carolina side. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

13

23. Newfound Gap Road, Fancy Ridge, overlook with ugly railing. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. Newfound Gap Road, Fancy Ridge, overlook with ugly railing. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

14

21. Newfound Gap Road, last pullout before gap, North Carolina ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

21. Newfound Gap Road, last pullout before gap, North Carolina side. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

15

13. Upper portal of tunnel on Newfound Gap Road looking ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Upper portal of tunnel on Newfound Gap Road looking S. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

16

20. Intersection of Newfound Gap Road and Clingman's Dome Road ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. Intersection of Newfound Gap Road and Clingman's Dome Road looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

17

14. Newfound Gap Road, view from atop tunnel, Tennessee side. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. Newfound Gap Road, view from atop tunnel, Tennessee side. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

18

27. Road view of revetment wall on Newfound Gap Road ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. Road view of revetment wall on Newfound Gap Road looking NNW. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

19

19. Detail of stairs at Newfound Gap parking area looking ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. Detail of stairs at Newfound Gap parking area looking NNE. - Great Smoky Mountains National Park Roads & Bridges, Newfound Gap Road, Between Gatlinburg, TN & Cherokee, NC, Gatlinburg, Sevier County, TN

20

40 CFR 81.345 - Utah.  

Code of Federal Regulations, 2010 CFR

... Remainder of Salt Lake County San Juan County Sanpete County Sevier County...County Piute County Rich County San Juan County Sanpete County Sevier County...County Piute County Rich County San Juan County Sanpete County Sevier...

2010-07-01

21

Influence of preexisting tectonic trends on geometries of Sevier orogenic belt and its foreland in Utah  

SciTech Connect

The tectonic style of the late Mesozoic Sevier orogenic belt in Utah was greatly affected by preexisting structural trends that date from the late Precambrian rifting and fragmentation of the North American continent. The late Precambrian cratonic margin (Cordilleran hinge line) was marked by a system of prominent faults including the north-south-trending ancestral Wasatch and ancient Ephraim faults and the southwest-northeast-trending Leamington, Scipio, Cove Fort, and Paragonah faults. During the Paleozoic and Mesozoic, renewed activity on these faults affected the geometries of the late Paleozoic Paradox and Oquirrh basins, the boundaries of the Jurassic Arapien Formation, and the sedimentary pattern of the Cretaceous foreland basin. Many of these fault zones were reactivated as tectonic ramps (e.g., the ancient Ephraim fault) and tear faults (e.g., the Leamington fault) during the compressional Sevier tectonism. The Fillmore arch and some other structural highs situated along the edge of the late Precambrian craton caused ramping of the inner Keystone-Pavant-Canyon thrust sheets and telescoping of the frontal thrust sheets. Post-thrust uplift of basement highs led to tectonic denudation and to the development of low-angle, extensional faults, such as the Sevier detachment. Northeast-trending lineaments, such as the Cove Fort and Paragonah lineaments, were reactivated as right-lateral strike-slip faults. They also affected the extent of the Marysvale volcanic field.

Picha, F.; Gibson, R.I.

1985-05-01

22

Preliminary Feasibility Assessment of Geologic Carbon Sequestration Potential for TVA's John Sevier and Kingston Power Plants  

SciTech Connect

This is a preliminary assessment of the potential for geologic carbon sequestration for the Tennessee Valley Authority's (TVA) John Sevier and Kingston power plants. The purpose of this assessment is to make a 'first cut' determination of whether there is sufficient potential for geologic carbon sequestration within 200 miles of the plants for TVA and Oak Ridge National Laboratory (ORNL) to proceed with a joint proposal for a larger project with a strong carbon management element. This assessment does not consider alternative technologies for carbon capture, but assumes the existence of a segregated CO{sub 2} stream suitable for sequestration.

Smith, Ellen D [ORNL; Saulsbury, Bo [ORNL

2008-03-01

23

Stratigraphy and structure of the Sevier thrust belt and proximal foreland-basin system in central Utah: A transect from the Sevier Desert to the Wasatch Plateau  

USGS Publications Warehouse

The Sevier orogenic belt in central Utah comprises four north-northwest trending thrust plates and two structural culminations that record crustal shortening and uplift in late Mesozoic and early Tertiary time. Synorogenic clastic rocks, mostly conglomerate and sandstone, exposed within the thrust belt were deposited in wedge-top and foredeep depozones within the proximal part of the foreland-basin system. The geologic relations preserved between thrust structures and synorogenic deposits demonstrate a foreland-breaking sequence of thrust deformation that was modified by minor out-of-sequence thrust displacement. Structural culminations in the interior part of the thrust belt deformed and uplifted some of the thrust sheets following their emplacement. Strata in the foreland basin indicate that the thrust sheets of central Utah were emplaced between latest Jurassic and Eocene time. The oldest strata of the foredeep depozone (Cedar Mountain Formation) are Neocomian and were derived from the hanging wall of the Canyon Range thrust. The foredeep depozone subsided most rapidly during Albian through Santonian or early Campanian time and accumulated about 2.5 km of conglomeratic strata (Indianola Group). The overlying North Horn Formation accumulated in a wedge-top basin from the Campanian to the Eocene and records propagation of the Gunnison thrust beneath the former foredeep. The Canyon Range Conglomerate of the Canyon Mountains, equivalent to the Indianola Group and the North Horn Formation, was deposited exclusively in a wedge-top setting on the Canyon Range and Pavant thrust sheets. This field trip, a three day, west-to-east traverse of the Sevier orogenic belt in central Utah, visits localities where timing of thrust structures is demonstrated by geometry of cross-cutting relations, growth strata associated with faults and folds, or deformation of foredeep deposits. Stops in the Canyon Mountains emphasize geometry of late structural culminations and relationships of the Canyon Range thrust to growth strata deposited in the wedge-top depozone. Stops in the San Pitch Mountains illustrate deposits of the foredeep depozone and younger, superjacent wedge-top depozone. Stops in the Sanpete Valley and western part of the Wasatch Plateau examine the evolution of the foreland-basin system from foredeep to wedge-top during growth of a triangle zone near the front of the Gunnison thrust.

Lawton, T. F.; Sprinkel, D. A.; Decelles, P. G.; Mitra, G.; Sussman, A. J.; Weiss, M. P.

1997-01-01

24

The Cave Canyon Detachment: A Standard for Deformation Expected of the Sevier Desert Detachment  

NASA Astrophysics Data System (ADS)

The Cave Canyon detachment (CCd)is a Neogene normal fault in the Mineral Mountains of west-central Utah. The Mineral Mountains are located directly south of the Sevier Desert basin. Because of its location, orientation and age constraints the Cave Canyon detachment has been proposed to be a non-active splay of the Sevier Desert detachment. The Sevier Desert detachment (SDd) is thought to be one of the largest displaced active normal faults in North America accruing as much as 47 km of displacement. There are some fundamental differences between the CCd and the SDd, one of those being that the CCd places Paleozoic limestone directly on a granitic pluton whereas the SDd beneath the basin places Neogene terrestrial sediments directly on Paleozoic limestones. Nevertheless, the temperature and depth at the time of movement are similar, thus making the CCd an appropriate analog to what might be expected of material potentially recovered by scientific drilling of the SDd. A suite of samples from above and below the CCd yields a distinctive pattern of brittle deformation. In the lower plate granites deformation is mostly brittle, and in the upper plate there is only a minor zone a few cm thick of mylonitized carbonate. The lower plate granites are characterized by an aureole of healed microfractures that decrease in abundance from the contact to background at over 100 m. Cataclasis is observed well over 100 m from the detachment, and is so intense at the contact that no grain is unaffected. Significant growth of new minerals and cross-cutting quartz veins are observed within a few meters of the detachment. In upper plate carbonate rocks, a cm-thick zone of dynamic recrystallization is observed, above which there is a meter or so of extensive undulose extinction of grains. Outward from this is a zone of strongly preferred oriented twinning. At a distance of 15 m from the contact only minor carbonate veinlets distinguish fault zone rock from unaffected limestone. If the SDd is an active or recently active fault zone with displacement as much as an order of magnitude greater than that of the CCd, and appreciably more deformation would be expected within the fault zone both above and below the detachment surface.

Anders, M. H.

2007-12-01

25

3. NEWFOUND GAP ROAD, VIEW OF LOOP OVER FROM CHIMNEY ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. NEWFOUND GAP ROAD, VIEW OF LOOP OVER FROM CHIMNEY TOPS. - Great Smoky Mountains National Park Roads & Bridges, The Loop Over Bridge, Newfound Gap Road 8.6 miles from park entrance, Gatlinburg, Sevier County, TN

26

2. NEWFOUND GAP ROAD, LOOP OVER, VIEW INSIDE OVERPASS. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. NEWFOUND GAP ROAD, LOOP OVER, VIEW INSIDE OVERPASS. - Great Smoky Mountains National Park Roads & Bridges, The Loop Over Bridge, Newfound Gap Road 8.6 miles from park entrance, Gatlinburg, Sevier County, TN

27

5. Abandoned mule trail tunnel. 1 mile from intersection with ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Abandoned mule trail tunnel. 1 mile from intersection with Newfound Gap Road looking SSE. - Great Smoky Mountains National Park Roads & Bridges, Clingmans Dome Road, Between Newfound Gap Road & Clingmans Dome, Gatlinburg, Sevier County, TN

28

2. Big Creek Road, worm fence and road at trailhead. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Big Creek Road, worm fence and road at trailhead. - Great Smoky Mountains National Park Roads & Bridges, Big Creek Road, Between State Route 284 & Big Creek Campground, Gatlinburg, Sevier County, TN

29

15. Gatlinburg spur with little Pigeon River, looking toward Pigeon ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. Gatlinburg spur with little Pigeon River, looking toward Pigeon Forge. - Great Smoky Mountains National Park Roads & Bridges, Foothills Parkway, From Chilowee to Walland & from Cosby to I-40, Gatlinburg, Sevier County, TN

30

75 FR 33765 - Foreign-Trade Zone 77-Memphis, TN, Application for Subzone, Delta Faucet Company (Faucets...  

Federal Register 2010, 2011, 2012, 2013

...Subzone, Delta Faucet Company (Faucets); Jackson, TN An application has been submitted...Faucet Company (Delta), located in Jackson, Tennessee. The application was submitted...located at 3441 Ridgecrest Road Ext., Jackson (Madison County), Tennessee. The...

2010-06-15

31

Role of crustal thickening and extensional collapse in the tectonic evolution of the Sevier-Laramide orogeny, western United States  

SciTech Connect

The effects of crustal thickening and extensional collapse of continental crust, combined with variations in plate-convergence forces, provide a coherent explanation for evolution of the Cretaceous-early Tertiary Sevier-Laramide orogeny. Crustal thickening of the Sevier-Laramide hinterland region was triggered by the combination of compressive plate-convergence forces and subduction-induced conductive heating of the crust. Eastward progradation of the locus of Sevier-Laramide deformation through time reflects the progressive widening of the region of crustal thickening. The region of maximum crustal thickening migrated outward until about 80 to 75 Ma, when it encountered excessively strong lithosphere of the Colorado Plateau. The locus of deformation was then transmitted laterally across both a hinterland that had attained maximum crustal thickness and the rigid Colorado Plateau, into the Laramide Rocky Mountain foreland. An episode of vigorous extensional collapse of orogenically thickened crust affected the southern Cordillera between about 75 and 35 Ma. Early Tertiary waning of plate-convergence rates and continued subduction-induced conductive heating of thickened crust allowed extensional collapse along the southern Cordillera region to drive the Colorado Plateau block northward. The result was the late Laramide phase of Rocky Mountain foreland deformation in the central and southern Laramide Rocky Mountain region continued through Eocene time, rather than ceasing at 56 Ma, as it did in the northern Cordillera.

Livaccari, R.F. (Univ. of New Mexico, Albuquerque (United States))

1991-11-01

32

Characterization of Tn5801.Sag, a Variant of Staphylococcus aureus Tn916 Family Transposon Tn5801 That Is Widespread in Clinical Isolates of Streptococcus agalactiae  

PubMed Central

Tn5801, originally detected in Staphylococcus aureus Mu50, is a Tn916 family element in which a unique int gene (int5801) replaces the int and xis genes in Tn916 (int916 and xis916). Among 62 tet(M)-positive tetracycline-resistant Streptococcus agalactiae isolates, 43 harbored Tn916, whereas 19 harbored a Tn5801-like element (Tn5801.Sag, ?20.6 kb). Tn5801.Sag was characterized (PCR mapping, partial sequencing, and chromosomal integration) and compared to other Tn5801-like elements. Similar to Tn5801 from S. aureus Mu50, tested in parallel, Tn5801.Sag was unable to undergo circularization and conjugal transfer. PMID:23817370

Mingoia, Marina; Morici, Eleonora; Tili, Emily; Giovanetti, Eleonora; Montanari, Maria Pia

2013-01-01

33

[Tn5037-a Tn21-like mercury transposon, detected in Thiobacillus ferrooxidans].  

PubMed

The 6645-bp mercury resistance transposon of the chemolithotrophic bacterium Thiobacillus ferrooxidans was cloned and sequenced. This transposon, named Tn5037, belongs to the Tn21 branch of the Tn21 subgroup, many members of which have been isolated from clinical sources. Having the minimum set of the genes (merRTPA), the mercury resistance operon of Tn5037 is organized similarly to most of the Gram-negative bacteria mer operons and is closest to that of Thiobacillus 3.2. The operator-promoter region of the mer operon of Tn5037 also has the common (Tn21/Tn501-like) structure. However, its inverted, presumably MerR protein binding repeats in the operator/promoter element are two base pairs shorter than in Tn21/Tn501. In the merA region, this transposon shares 77.4, 79.1, 83.2 and 87.8% identical bases with Tn21, Tn501, T. ferrooxidance E-15, and Thiobacillus 3.2, respectively. No inducibility of the Tn5037 mer operon was detected in the in vivo experiments. The transposition system (terminal repeats plus gene tnpA) of Tn5037 was inactive in Escherichia coli K12, in contrast to its resolution system (res site plus gene tnpR). However, transposition of Tn5037 in this host was provided by the tnpA gene of Tn5036, a member of the Tn21 subgroup. Sequence analysis of the Tn5037 res site suggested its recombinant nature. PMID:11642118

Kaliaeva, E S; Kholodi?, G Ia; Bass, I A; Gorlenko, Zh M; Iur'eva, O V; Nikiforov, V G

2001-08-01

34

3. BIG HOUSE (left) AND CORN CRIB (right) IN THE ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. BIG HOUSE (left) AND CORN CRIB (right) IN THE BACKGROUND. See also individual HABS documentation: Walker Family Farm, Big House (HABS No. TN-121 A), and Walker Family Farm, Corn Crib (HABS No. TN-121 C). - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

35

Climatic Research Unit Percentage of cold nights (Tn10p) and warm nights (Tn90p)  

E-print Network

Climatic Research Unit Percentage of cold nights (Tn10p) and warm nights (Tn90p) & percentage of cold days (Tx10p) and warm days (Tx90p) 1950-2004 Trends in warm nights (Tn90p) 1950-2004 Climate and Atmospheric Climate Change. In: Climate Change 2007: The Physical Science Basis. Working Group I. 4th IPCC

Feigon, Brooke

36

Spatial and Temporal Distribution of Strain in the Sevier Desert Region from a Decade of BARGEN Continuous GPS Observations  

NASA Astrophysics Data System (ADS)

A transect of four BARGEN GPS sites from 1997-2000 at latitude 39N revealed a linear increase in velocity from 0 mm/yr on the Colorado Plateau to ~4 mm/yr in east-central Nevada. These geodetic data, when combined with paleoseismic and neotectonic observations, were used to argue for present-day strain accumulation on the Sevier Desert detachment, a seismically-imaged, low-angle (12) normal fault that underlies a significant portion of west central Utah [Niemi et al., 2004]. A new GPS velocity solution [Davis et al., 2006], including 5 additional years of observations and a new GPS station in the eastern half of the transect, serves to both clarify and complicate our understanding of the spatial and temporal distribution of strain in this region. Geodetic velocity gradients among the three original eastern sites (from east to west, CAST, SMEL, and FOOT), and including the new site, SPIC, located between CAST and SMEL, continue to suggest a linear strain gradient from the Colorado Plateau to westernmost Utah, on a transect spanning the Wasatch, Sevier Desert, and House Range normal faults. In contrast, the baseline between FOOT and EGAN, which underwent extension from 1997-2000, began to contract in 2000, as site EGAN slowed with respect to the Colorado Plateau. This deviation in velocity continued until 2003, when EGAN began moving westward, and has, as of 2006, returned to near its pre-2000 velocity with respect to the Colorado Plateau. The eastward excursion of GPS site EGAN is not unique, and similar excursions are observed in the time series of all BARGEN GPS sites that lie west of ~114.25W over the time period 2000-2003 [Davis et al., 2006]. The origin of this velocity anomaly is uncertain, but the magnitude and spatial extent of the excursion, as well as the observation of a deep earthquake swarm (~30-40 km depth) coincident with dramatic motion of GPS site SLID, near Lake Tahoe, in 2003 [Smith et al., 2004] suggest a deep crustal or mantle influence on the observed GPS velocities. Bright, mid-crustal horizontal reflections observed in COCORP reflection seismic data west of 114W have been postulated to represent a detachment that could accommodate shearing along the base of the crust in Nevada [Hauser et al., 1987], a hypothesis broadly consistent with the observed GPS velocities, with the crust shearing relatively east over the subcrustal lithosphere. A deep observatory in the Sevier Desert region, transecting the Sevier Desert detachment, would complement existing paleoseismic and neotectonic studies, and the decade of BARGEN continuous GPS observations, as well as new GPS data from 8 Earthscope Plate Boundary Observatory sites installed along this transect in the past few years. Key questions that could be assessed with a deep observatory are how slip, and strain, at depth on an inclined fault are reflected in geodetic observations of strain at the surface; whether the state of stress and strain rate on the Sevier Desert detachment change through time, as suggested by long-term periodicity in strain release as recorded in the geologic record, and what effect long-lived velocity excursions, such as observed from 2000-2003, have on the regional stress state, perhaps leading to a clearer understanding of the source of these anomalies, and the aseismic tectonic behavior of the lithosphere.

Niemi, N. A.; Wernicke, B. P.

2007-12-01

37

Reinterpretation of relationships among Keystone thrust, Red Springs thrust, contact thrust, and Cottonwood fault, Clark County, Nevada  

Microsoft Academic Search

The basin-range and Sevier tectonic events are well-documented in Clark County, Nevada. Other tectonic events have been interpreted from structural relationships in the Spring Mountains. These include an early thrust-faulting event and a high-angle faulting event that occurred between emplacement of the early thrust and emplacement of the Keystone thrust. The results of this study indicate that there was not

Vincent Matthews; III

1985-01-01

38

77 FR 17560 - Tennessee Disaster #TN-00063  

Federal Register 2010, 2011, 2012, 2013

...disaster for the State of Tennessee (FEMA-4060-DR...the disaster: Primary Counties (Physical Damage and...Mcminn, Monroe, Overton, Polk. Contiguous Counties (Economic Injury Loans Only): Tennessee: Bledsoe,...

2012-03-26

39

75 FR 55833 - Tennessee Disaster #TN-00042  

Federal Register 2010, 2011, 2012, 2013

...a disaster for the State of Tennessee dated 09/07/2010. Incident...affected by the disaster: Primary Counties: Putnam. Contiguous Counties: Tennessee: Cumberland, Dekalb, Fentress, Jackson, Overton, Smith, White. The...

2010-09-14

40

Detection of the Tn antigen in leukaemia using monoclonal anti-Tn antibody and immunohistochemistry.  

PubMed

Exposure of the normally cryptic Tn antigen on haemopoietic cells leading to erythrocyte polyagglutination has been reported in a few cases of malignant or premalignant haemopoietic disorders and has been attributed to a selective deficiency of the enzyme 3-beta-D-galactosyl-transferase. A male patient presented with acute myelomonocytic leukaemia with no evidence of Tn expression but, 16 months later, in the terminal stage of the disease, the majority of the erythrocytes were found to be polyagglutinable. Tn expression was confirmed by the use of lectins and by agglutination with a Tn-specific monoclonal antibody, FBT3. Retrospective studies of stored blood and bone marrow smears were performed by immunocytochemistry using FBT3. Tn positive cells were first detected in the marrow 8 months prior to death. They increased progressively in number and, in the terminal illness, over 90% of erythroid precursors in the marrow and erythrocytes in peripheral blood and 40% of granulocyte precursors of the marrow and 10% of granulocytes in the blood were Tn positive. These observations suggest that Tn expression was present in a subclone of cells which became dominant during the course of the disease and that there may be a relationship between Tn expression and leukaemic progression. PMID:3479188

Roxby, D J; Morley, A A; Burpee, M

1987-10-01

41

HOSA National Leadership Conference Nashville, TN  

E-print Network

HOSA National Leadership Conference Nashville, TN June 22 - June 25, 2005 Gaylord Opryland Resort National Leadership Conference information from National HOSA and the NJ HOSA office. Please note the new, please include the following: · HOSA National Leadership Conference form (Pages 1 & 2) · Medical

Rusu, Adrian

42

Structural styles of the Guess Creek fault block beneath the Great Smoky thrust sheet, Blount County, Tennessee  

SciTech Connect

A road cut along US 321 N, approximately 1 km NW of Walland, TN, exposes a previously unexposed complexly deformed section of Middle Ordovician clastic wedge [Chickamauga Group, Sevier Shale] sedimentary rocks. It provides an excellent opportunity to analyze both the lithologic assemblages and complex folding and faulting beneath the Great Smoky thrust sheet. Arkosic quartzite of the Lower Cambrian Cochran Conglomerate [Chilhowee Group], has been thrust over weaker Sevier Shale in the hanging wall of the Guess Creek fault. Regionally, the Great Smoky fault separates metamorphosed Precambrian to Lower Cambrian clastic shelf, slope, and rift facies rocks of the western Blue Ridge from Cambro-Ordovician carbonate shelf and orogenic wedge deposits of the foreland fold and thrust belt. West of the Great Smoky fault, the Guess Creek fault has been interpreted to floor duplexed Cambro-Ordovician rocks exposed in windows beneath the Great Smoky thrust sheet in the vicinity of the Great Smoky Mountains National Park. The Sevier Shale here consists of variably cleaved shale, siltstone, sandstone, and conglomerate. It exhibits a variety of fold styles throughout the exposure, ranging from predominantly noncylindrical tight folds to broad, open structures. A weak axial-planar pencil cleavage is developed in the Middle Ordovician shale and siltstone, along with a secondary cleavage that transects the axial surfaces of the folds. Minor thrust faults within the Sevier Shale appear to have formed by propagation through tightened fold hinges or bedding-parallel slip. The fold pattern observed in the roadcut appears to be partly the result of movement along a tear fault that broke both the hanging wall and footwall of the Great Smoky thrust sheet after emplacement. Slickenline orientations along minor thrust surfaces in the Cochran Conglomerate indicate eastward-directed, oblique-slip movement of the tear fault.

Carter, M.W.; Davidson, G.L.; Heller, J.A. (Univ. of Tennessee, Knoxville, TN (United States). Dept. of Geological Sciences); Hatcher, R.D. Jr. (Oak Ridge National Lab., TN (United States))

1993-03-01

43

Low-temperature thermochronology of the Laramide Ranges and eastward translation of shortening in the Sevier Belt, Wyoming, Utah and Montana  

Microsoft Academic Search

This dissertation contains two studies that use very different techniques to investigate the Mesozoic and Cenozoic tectonics of the western USA. The first study investigates shortening in the Sevier thrust belt of northeast Utah and southwest Wyoming using cross sections and seismic reflection data. The second study investigates the low-temperature thermochronology of the Laramide Ranges using apatite (U-Th)\\/He dating. We

Sara Lynn Peyton

2009-01-01

44

Transformation of Mycoplasma gallisepticum with Tn916, Tn4001, and integrative plasmid vectors.  

PubMed Central

Mycoplasma gallisepticum causes respiratory disease in avian species, but little is known about its mechanism(s) of pathogenesis. These studies were undertaken in order to develop genetic systems for analysis of potential virulence factors. M. gallisepticum was transformed with plasmids containing one of the gram-positive transposons Tn916 or Tn4001, which inserted randomly into the mycoplasmal chromosome. Plasmids containing cloned chromosomal DNA were also constructed and tested for integration into regions of DNA homology derived either from chromosomal fragments or from the gentamicin resistance marker from Tn4001. These studies demonstrate that M. gallisepticum is amenable to transformation with both transposons and integrative vectors. Images PMID:8021232

Cao, J.; Kapke, P. A.; Minion, F. C.

1994-01-01

45

Deformation characteristics of the Sheeprock and Canyon Range thrust sheets (Sevier orogenic belt) based on microstructural and strain studies  

SciTech Connect

The condition of emplacement of internal thrust sheets in the Sevier fold-and-thrust belt can be characterized by detailed studies on deformation of quartzites within these sheets. The Sheeprock and Canyon Range thrust sheets in north-central Utah both carry Proterozoic Caddy Canyon and Mutual quartzites in their hanging walls. The Caddy Canyon is a medium to coarse-grained quartzite with subrounded grains and some grain-overgrowth. The Mutual is typically coarse-grained to pebbly, has subrounded to well rounded grains with overgrowths, and is characterized by clasts showing an older metamorphic (in some cases, mylonitic) fabric. In the Sheeprock sheet both quartzites show evidence for considerable crystal-plastic deformation, including deformation lamellae, undulose extinction, and development of subgrains; the Caddy Canyon also shows considerable pressure solution. Late stage cataclasis, characterized by the development of cemented cataclasites, is also evident. In the Canyon Range, both quartzites show less plastic deformation, although the Caddy Canyon shows large amounts of pressure solution. The late stage cataclasites are coherent but not cemented, and there is locally strong plastic deformation adjacent to the cataclasite zones. The microstructures suggest an overall shallower level of deformation for the Canyon Range sheet. Strains in the quartzites are quantified using the Fry technique, and the strains are partitioned into plastic and diffusional components using deformed rutile needles within quartz grains and pressure-solved grain shapes.

Sussman, A.J.; Mitra, G. (Univ. of Rochester, NY (United States). Dept. of Geological Sciences)

1993-04-01

46

Characterization of the staphylococcal beta-lactamase transposon Tn552.  

PubMed Central

The staphylococcal beta-lactamase transposon Tn552 is a member of a novel group of transposable elements. The organization of genes in Tn552 resembles that of members of the Tn21 sub-group of Tn3 family transposons, which transpose replicatively by cointegrate formation and resolution. Thus, a possible resolution site ('resL') and a resolvase gene (tnpR or 'binL') have been identified. However, consistent with the fact that Tn552 generates 6 bp (rather than 5 bp) flanking direct repeats of target DNA, neither the putative transposase protein, nor the terminal inverted repeats of Tn552 are homologous to those of Tn3 elements. Tn552, like phage Mu and retroelements, is defined by the terminal dinucleotides 5' TG .. CA 3'. A naturally occurring staphylococcal plasmid, pI9789, contains a Tn552-derived resolution system ('resR-binR') that acts as a 'hotspot' for Tn552 transposition; insertion creates a segment of DNA flanked by inversely repeated resolution sites, one (resR) on pI9789 and the other (resL) on Tn552. The putative Tn552 resolvase, the most closely related of known resolvases to the homologous DNA invertases, initially was identified as a DNA invertase ('Bin') as a result of its ability to mediate efficient inversion of this segment in vivo. PMID:2555186

Rowland, S J; Dyke, K G

1989-01-01

47

Licensing the TN-40 at Prairie Island  

SciTech Connect

Northern States Power Company (SNP) is currently in the process of licensing an independent spent-fuel storage installation (ISFSI) at its Prairie Island nuclear generating plant. The proposed facility would utilize the TN-40 dry-metal storage casks, each holding 40 Prairie Island spent-fuel assemblies. The ISFSI consists of two concrete pads with a combined capacity of 48 casks for a total capacity of 1,920 fuel assemblies. In addition to a 10CRF72 license from the US Nuclear Regulatory Commission (NRC), a certificate of need from the Minnesota Public Utilities Commission is required by state law.

Kapitz, J.; McCarten, L. (Northern States Power Co., Welch, MN (United States))

1991-11-01

48

Reinterpretation of relationships among Keystone thrust, Red Springs thrust, contact thrust, and Cottonwood fault, Clark County, Nevada  

SciTech Connect

The basin-range and Sevier tectonic events are well-documented in Clark County, Nevada. Other tectonic events have been interpreted from structural relationships in the Spring Mountains. These include an early thrust-faulting event and a high-angle faulting event that occurred between emplacement of the early thrust and emplacement of the Keystone thrust. The results of this study indicate that there was not an early thrust event nor was there high-angle faulting prior to the Sevier deformational event. The Cottonwood fault and the Contact thrust in the Spring Mountains are interpreted here as a lateral ramp and floor thrust beneath a duplex fault zone. The Keystone thrust forms the roof thrust for the duplex fault zone that bows up the upper plate of the Keystone thrust. The Red Springs thrust is interpreted as the Keystone thrust, which was broken and differentially rotated during Neogene oroclinal bending associated with the Las Vegas shear zone. The structural relationships in the Spring Mountains do not require any Mesozoic or Cenozoic deformational episodes other than the well-known Sevier and basin-range events.

Matthews, V. III

1985-05-01

49

Jurassic thrust burial in the Funeral Mountains of the Sevier retroarc: Linking P-T paths and Lu-Hf garnet geochronology  

NASA Astrophysics Data System (ADS)

Late Jurassic to early Cenozoic eastward subduction of the Farallon plate beneath the western margin of the North American plate led to development of the classic elements of the noncollisional two-sided Cordilleran orogen of the western US. These include from west to east, the Franciscan accretionary complex, Great Valley forearc basin, Sierran magmatic arc, and the retroarc Sevier orogenic wedge, including its metamorphic hinterland. Crustal shortening and mountain building during this noncollisional orogeny are thought to have resulted from coupling at the interface between the eastward underflowing Farallon plate and the westward moving North America plate. While the timing of Sierran arc magmatism, Great Valley forearc basin sedimentation, and Franciscan trench sedimentation and subduction metamorphism are increasingly well understood, our understanding of the timing of episodic contractional deformation in the retroarc region remains incomplete, in particular for the metamorphic hinterland. This has limited our ability to link forearc, arc, and retroarc processes, and to differentiate retroarc deformation events related to noncollisional orogenesis from shortening due to older collisional events in the late Middle to early Late Jurassic. The influence of collisional events in the forearc region -- including collision of exotic and/or fringing arcs, ridge collision, or subduction termination and inception -- on deformation in the retroarc region may be under appreciated. Lu-Hf dating and P-T path determination of prograde garnet growth, from the hinterland of the Sevier orogen in the Funeral Mountains of southeastern California, document late Middle to early Late Jurassic thrust burial; importantly, this shortening predates development of the Franciscan accretionary wedge and sedimentation in the Great Valley forearc basin. Lu-Hf garnet geochronology of middle greenschist facies pelitic schist of the Johnnie Formation of the southeastern Funeral Mountains core complex yields a 5-point garnet and whole rock isochron age of 162.1 5.8 Ma (2?). Composite PT paths determined from growth-zoned garnets from the same sample show a nearly isothermal pressure increase of ~2 kbar at ~490C, suggesting thrust burial at 162.1 5.8 Ma. Subjacent chloritoid schist and marble yield muscovite argon-argon plateau ages of 152.6 1.4 Ma and 146.0 1.1 Ma, respectively, documenting post-burial cooling in the Late Jurassic. Recognition of Late Jurassic tectonic burial in the Death Valley region suggests that the spatial distinction between "Sevier" and western older Jurassic deformation provinces becomes obscured to the south as the magmatic arc and the miogeoclinal hinge line converge. Furthermore, collision-driven shortening may be more important than currently recognized for the Jurassic tectonics of western North America, raising caution in assigning deformation to the noncollisional Sevier orogeny.

Wells, M. L.; Hoisch, T. D.; Beyene, M.; Vervoort, J. D.

2011-12-01

50

Expression of a Tn-like epitope by carcinoma cells.  

PubMed

A monoclonal antibody, FBT3, was raised against Tn positive erythrocytes and, using immunohistochemistry, fresh and fixed tissues from patients with cancer were studied to detect any expression of a Tn-like epitope. Expression was found in neoplastic cells, usually both in cytoplasm and on cell membranes, from 104 of 147 cases of carcinoma and 1 of 13 cases of lymphoma, but rarely in adjacent, morphologically normal cells. Tn expression was seen in some normal glandular cells but, unlike cancer cells, it was distributed as fine granules in a supranuclear position. Detection of a Tn-like epitope is of theoretical interest and may be of direct diagnostic value. PMID:2449235

Roxby, D J; Skinner, J M; Morley, A A; Weeks, S; Burpee, M

1987-12-01

51

48 CFR 1201.301-72 - TAC or TN numbering.  

Code of Federal Regulations, 2010 CFR

...Regulations System 5 2010-10-01 2010-10-01 false TAC or TN numbering. 1201.301-72 Section 1201.301-72...REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered...

2010-10-01

52

48 CFR 1201.301-72 - TAC or TN numbering.  

Code of Federal Regulations, 2012 CFR

...Regulations System 5 2012-10-01 2012-10-01 false TAC or TN numbering. 1201.301-72 Section 1201.301-72...REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered...

2012-10-01

53

48 CFR 1201.301-72 - TAC or TN numbering.  

Code of Federal Regulations, 2013 CFR

...Regulations System 5 2013-10-01 2013-10-01 false TAC or TN numbering. 1201.301-72 Section 1201.301-72...REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered...

2013-10-01

54

48 CFR 1201.301-72 - TAC or TN numbering.  

Code of Federal Regulations, 2011 CFR

...Regulations System 5 2011-10-01 2011-10-01 false TAC or TN numbering. 1201.301-72 Section 1201.301-72...REGULATIONS SYSTEM Agency Acquisition Regulations 1201.301-72 TAC or TN numbering. TACs and TNs will be numbered...

2011-10-01

55

Characterization of a TnMAVS protein from Tetraodon nigroviridis.  

PubMed

A growing family of cellular proteins encoding for caspase activation and the recruitment domain (CARD) plays a crucial role in immunity by sensing viral infections and signaling antiviral immune defenses. We obtained a MAVS-like protein (named TnMAVS) from Tetradon nigroviridis, which contains a CARD domain, a pro-rich domain, and a TM domain similar to human MAVS. A fluorescence assay showed that TnMAVS was located in the cytoplasm and near by the membrane, and not the mitochondria in FHM cells. As such, it was considered as a new member of MAVS. The TnMAVS was highly expressed in the liver and muscle of T. nigroviridis. In the spleen, TnMAVS was down-regulated when the fish was treated with polyinosinic:polycytidylic acid or challenged with ISKNV, but was not affected by PGN or LPS. The dual luciferase reporter assay revealed that TnMAVS overexpression resulted in the activation of the interferon-sensitive response element and NF-?B signal pathways. In addition, a characteristic TRAF3-associated peptide PVQD was found in the TnMAVS sequence. Co-immunoprecipitation assays indicated that TnMAVS could interact with zfTRAF3 in eukaryotic cells. PMID:21527281

Xiang, Zhiming; Qi, Lin; Chen, Weijian; Dong, Chuanfu; Liu, Zhaoyu; Liu, Dong; Huang, Mengli; Li, Wei; Yang, Gan; Weng, Shaoping; He, Jianguo

2011-11-01

56

Optimization Online - SESOP-TN: Combining Sequential Subspace ...  

E-print Network

Sep 29, 2008 ... Abstract: SESOP-TN is a method for very large scale unconstrained ... search with subspace optimization, we allow Conjugate Gradient (CG) iterations to ... Matlab code is available at http://ie.technion.ac.il/~mcib/sesoptn.html.

Michael Zibulevsky

2008-09-29

57

NMR structure of the Tn916 integraseDNA complex  

Microsoft Academic Search

The integrase protein catalyzes the excision and integration of the Tn 916 conjugative transposon, a promiscuous genetic element that spreads antibiotic resistance in pathogenic bacteria. The solution structure of the N-terminal domain of the Tn916 integrase protein bound to its DNA-binding site within the transposon arm has been determined. The structure reveals an interesting mode of DNA recognition, in which

Jonathan M. Wojciak; Kevin M. Connolly; Robert T. Clubb

1999-01-01

58

78 FR 52424 - Amendment of Class E Airspace; Dayton, TN, Establishment of Class E Airspace; Cleveland, TN, and...  

Federal Register 2010, 2011, 2012, 2013

...establishes Class E Airspace at Cleveland Regional Jetport, Cleveland, TN, to accommodate area navigation (RNAV) global positioning system (GPS) SIAPs at the airport. Information regarding Bradley Memorial Hospital is added to the Cleveland,...

2013-08-23

59

Magnitude, Timing, and Geometry of Extension in the Southern Sevier Desert Basin From Piercing Points, Seismic-Stratigraphic Reconstruction, and Deep well Data  

NASA Astrophysics Data System (ADS)

Palinspastic reconstruction of Mesozoic thrust sheets provides the main constraint for an estimated 47 km of Cenozoic extensional displacement along the Sevier Desert detachment (SDD) in the central Sevier Desert Basin. Hanging wall and footwall piercing points indicate that the SDD accommodated a minimum of 35 km of extensional displacement in the narrower southern part of the basin. The piercing points for the SDD are defined by the intersection of the SDD, the Canyon Range thrust (CRT), and a regional early Cenozoic erosion surface (ES). The hanging wall piercing point lies immediately northeast of the Cricket Mountains, where the SDD-CRT- ES intersection is narrowly defined by intersecting structure maps derived from published seismic reflection data. The footwall piercing point lies in the southern foothills of the Canyon Range, where the SDD breakaway plane is well constrained by an industry seismic line that lies within 2 km of the exposed intersection of the CRT with the base of the Oligocene Oak City Formation. Timing of extension in the southern Sevier Desert basin is constrained by a kinematic reconstruction of detachment and imbricate fault displacement, footwall uplift, and supradetachment sedimentation for Oligocene, Miocene, and Plio-Pleistocene seismic sequences. The reconstruction is centered on a seismic reflection and gravity interpretation along the published Pan Canadian profiles 2 and 3 that is tied to dated intervals in six industry wells. Fault restoration indicates that Oligocene and Miocene phases of slip each accounted for about 40 percent of the total displacement. Simultaneous backstripping of the Oligocene, Miocene, and Plio-Pleistocene supradetachment sequences records hanging wall subsidence simultaneous with footwall uplift, with a footwall burial history that is consistent with published Miocene apatite and zircon fission-track ages of footwall samples. The geometric evolution of the southern SDD extensional system is consistent with its development above a broad westward-migrating "rolling hinge" zone associated with isostatic uplift of the detachment footwall. Hanging wall normal faults east of the footwall crest exhibit small post-Miocene displacement, with demonstrable Quaternary slip restricted to the crest and western limb of the uplift, most notably along the Black Rock and Clear Lake fault zones. Early abandonment of the eastern part of the detachment may explain the indistinct geomorphic and structural expression of the break-away zone at the surface. The deepest level of the southern SDD also presents a complex geometry and kinematic history. The 1996 Chevron 1-29 Black Rock Federal well through the western basin margin penetrated a normal fault that places Jurassic over lower Cambrian strata at 4650 m measured depth, well above the principal SDD seismic reflection. The fault is not correlated to any large- displacement high-angle fault at shallow levels, and may form the abandoned roof to an extensional duplex.

Coogan, J. C.; Decelles, P. G.

2007-12-01

60

75 FR 27846 - Tennessee Disaster Number TN-00039  

Federal Register 2010, 2011, 2012, 2013

...declaration for the State of Tennessee, dated 05/04/2010...the disaster: Primary Counties: (Physical Damage and...Wilson. Contiguous Counties: (Economic Injury Loans Only): Tennessee: Overton, Pickett, Putnam,...

2010-05-18

61

From Source to Sink: Exhumation of the North America Cordillera Revealed by Multi-dating of Detrital Minerals from Upper Jurassic-Upper Cretaceous Sevier Foreland Basin  

NASA Astrophysics Data System (ADS)

We sampled twenty-two Late Jurassic to Late Cretaceous syn-orogenic conglomerate clasts in proximal units in the Sevier fold-thrust belt and their distal sandstone equivalents up to 300 km east of the thrust front, in Utah, Colorado, Wyoming, and South Dakota. To better constrain depositional ages, these samples were analyzed using detrital zircon U-Pb (DZ U-Pb) geochronology. To identify a thermochronometer that measures source exhumation in the North America Cordillera, both zircon (U-Th)/He and apatite fission track (AFT) thermochronology was utilized, on both the conglomerate cobbles and sandstone (detrital) samples. Eleven samples were analyzed with zircon (U-Th)/He; however, discordant ages in the conglomerate cobble samples suggest that this system was not fully reset and never experienced T> ~180 C in the source stratigraphy during the Sevier orogeny. Eleven other samples are analyzed using apatite fission track thermochronology (AFT); AFT ages are generally similar or older than depositional ages indicating that the detrital ages record source exhumation signals, and that exhumation depth corresponds to T>~120 C. In order to test whether or not the youngest cooling AFT age population represents a source exhumation signal or a co-magmatic signal we here performed double dating of the detrital AFT samples using apatite U-Pb thermochronology. Maximum depositional ages using DZ U-Pb match existing age controls on basin stratigraphy. Our study shows that AFT is an effective thermochronometer to detect source exhumation for Cretaceous foreland stratigraphy in the western U.S.A. Lag-times (i.e. the difference between the source exhumation age and depositional age) are ~0 to 5 Myr with relatively steady-state to slightly increasing exhumation rates suggesting orogenic growth at this time. The very short lag times also indicate limited to no storage time between source and sink. The AFT lag time of the Early Cretaceous Kelvin Formation is ~5 Myr and represents a time when the Canyon Range Thrust was active to the southwest. Upper Cretaceous units have an approximate lag time of 0 Myr, during a time when the Willard, Crawford, Medicine Butte and Absaroka Thrust systems were active to the west. These lag-time measurements correspond to exhumation rates of ~0.8 mm/yr to >>1 mm/yr.

Painter, C. S.; Carrapa, B.; DeCelles, P. G.; Gehrels, G. E.; Thomson, S. N.

2013-12-01

62

SESOP-TN: Combining Sequential Subspace Optimization with Truncated Newton method  

Microsoft Academic Search

We present a method for very large scale unconstrained optimization of smooth func- tions. It combines ideas of Sequential Subspace Optimization (SESOP) (4, 2) with those of the Truncated Newton (TN) method. Replacing TN line search with sub- space optimization, we allow Conjugate Gradient (CG) iterations to stay matched through consequent TN steps. This resolves the problem of TN sensitivity

Michael Zibulevsky

63

Location of an ampicillin resistance transposon, Tn1701, in a group of small, nontransferring plasmids.  

PubMed Central

By restriction endonuclease cleavage mapping and electron microscopic examination of heteroduplexes, we have identified an ampicillin resistance determinant transposon, designated Tn1701, in a group of small, nontransferring plasmids which confer resistance to ampicillin (Ap), sulfonamide (Su), and streptomycin (Sm). Plasmid NTP1, which mediates Ap resistance, contains Tn1701. Recombinant plasmids NTP3 (Ap Su) and NTP4 (Ap Su Sm) contain Tn1701, indicating that they were derived by transposition of Tn1701 from NTP1 to an unrelated plasmid, NTP2 (Su Sm). The transposon Tn1701 is very similar to the known ampicillin resistance transposons Tn1, Tn2, and Tn3 in its size (3.2 x 10(6) daltons), base sequence homology observed by heteroduplex formation, restriction endonuclease cleavage sites, and possession of a short inverted repeat sequence at both ends. Like the other TnA elements, Tn1701 also specifies a type TEM beta-lactamase. Images PMID:370108

Yamada, Y; Calame, K L; Grindley, J N; Nakada, D

1979-01-01

64

MIDDLE TN RES & ED CTR UNIV OF TN BULL TEST DR. F. DAVID KIRKPATRICK BOX 160 2011-12 SR BULLS ANIMAL SCIENCE DEPT  

E-print Network

TEST SITE: MIDDLE TN RES & ED CTR UNIV OF TN BULL TEST DR. F. DAVID KIRKPATRICK BOX 160 2011-12 SR I .6 I 44 I 24 708 46.0 2.36 BGS ANGUS 213 CRUSSELL DR--PINEY FLATS, TN 37686 TELE:423-538-6792 7 9 ANGUS 111 BRENTWOOD DR--JOHNSON CITY, TN 37601 TELE:423-773-3396 10 SITZ UPWARD 307R 10/ 4/** X65 . I 1

Tennessee, University of

65

EMPLOYMENT SUMMARY FOR 2011 GRADUATES Memphis, TN 38103  

E-print Network

EMPLOYMENT SUMMARY FOR 2011 GRADUATES Memphis, TN 38103 Website : www.memphis.edu/law/ Phone : 901 graduates 129 Unemployed - Not Seeking 2 Employment Status Unknown 22 Unemployed - Seeking 9 Employed - Undeterminable * 0 0 0 0 0 Employed - Bar Passage Required 77 2 2 0 81 Pursuing Graduate Degree Full Time 1

Dasgupta, Dipankar

66

ERDC TN-EMRRP-EI-03 September 2008  

E-print Network

ERDC TN-EMRRP-EI-03 September 2008 Short-Term Sediment Burial Effects on the Seagrass Phyllospadix modification (Short and Wyllie- Echeverria 1996; Orth et al. 2006). Seagrasses in the genus Phyllospadix, also and subtidal environments (den Hartog 1970, Phillips and Meñez 1988). Like other seagrasses, Phyllospadix spp

Carrington, Emily

67

ERDC/TN APCRP-BC-20 December 2010  

E-print Network

ERDC/TN APCRP-BC-20 December 2010 Relationship Between Eurasian Watermilfoil Phenology relationships in the grasses indicating that endophytes can have dramatic biological effects on growth cite another important factor that could be involved: the presence or absence of mutualistic endophytes

US Army Corps of Engineers

68

DNA sequence analysis of the transposon Tn3: Three genes and three sites involved in transposition of Tn3  

Microsoft Academic Search

Summary The complete nucleotide sequence of the transpo- son Tn3 and of 20 mutations which affect its trans- position are reported. The mutations, generated in vitro by random insertion of synthetic restriction sites, proved to contain small duplications or deie- tions immediately adjacent to the new restriction site. By determining the phenotype and DNA se- quence of these mutations we

Fred Heffron; Brian J. McCarthy; Hisako Ohtsubo; Eiichi Ohtsubo

1979-01-01

69

Stewart K. McCroskey Memorial Fund Social Capital: Processes and Effects in Knox County, TN  

E-print Network

prominent in low-income communities in Knoxville. Problem Social capital is defined as the use of social mobility as the quality of such social relationships is affected by fault lines of society such as racism

Grissino-Mayer, Henri D.

70

Lightcurve Analysis of Near-Earth Asteroid 2010 TN54  

NASA Astrophysics Data System (ADS)

CCD photometry observations of the near-Earth asteroid (NEA) 2010 TN 54 indicate a period of either 6.14 h or 12.12 h, depending on whether a monomodal or bimodal lightcurve is adopted. The amplitude was only 0.07 0.01 mag, which - along with the period being nearly commensurate with an Earth day - made finding a definitive solution difficult, despite being observed from locations in North America and Europe.

Warner, Brian D.; Benishek, Vladimir; Ferrero, Andrea

2014-01-01

71

78 FR 27029 - Modification of Class C Airspace; Nashville International Airport; TN  

Federal Register 2010, 2011, 2012, 2013

...No. 12-AWA-7] Modification of Class C Airspace; Nashville International Airport...Nashville International Airport, TN, Class C airspace area by removing a cutout from the...Nashville International Airport, TN, Class C airspace area (78 FR 6257)....

2013-05-09

72

mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320  

Microsoft Academic Search

We previously constructed a series of mini-Tn7 chromosome integration vectors that, when provided only with the site-specific transposition machinery, generally transpose to a naturally evolved, neutral attTn7 site that is located 25-bp downstream of the glmS gene. Here we provide a protocol for application of the mini-Tn7 system in Proteus mirabilis as an example of a bacterium with a secondary

Kyoung-Hee Choi; Herbert P Schweizer

2006-01-01

73

Presence of chromosomal elements resembling the composite structure Tn3701 in streptococci.  

PubMed Central

Tn3701, carried by Streptococcus pyogenes A454, is the first chromosomal composite element to be described; it contains in its central region Tn3703, a transposon similar to Tn916. A comparison by DNA-DNA hybridization of Tn3701 with omega(cat-tet) and Tn3951, carried by Streptococcus pneumoniae BM6001 and by Streptococcus agalactiae B109, respectively, revealed that the two latter structures are also Tn3701-like composite elements. The DNAs of 27 other antibiotic-resistant group A, B, C, and G streptococci and of S. pneumoniae BM4200 showed sequence homologies to Tn3701 (14 strains, including BM4200), to the regions of Tn3701 outside of Tn3703 (5 strains), and to Tn916 alone (8 strains). The DNAs of five strains did not detectably hybridize with any probe. The tetM gene was identified in most chromosomal genetic elements coding for tetracycline-minocycline resistance. Since Tn3701-like elements are widely disseminated among antibiotic-resistant streptococci (47% of the 34 strains studied), we propose that Tn3701 be considered the prototype of chromosomal composite elements. Images FIG. 2 PMID:2153659

Le Bouguenec, C; de Cespedes, G; Horaud, T

1990-01-01

74

A comparison of Anisotropy of Magnetic Susceptibility Studies to Clast Based Strain Analysis in Sandstones, From The Outer Margin Of The Sevier Orogenic Foreland, Western Wyoming.  

NASA Astrophysics Data System (ADS)

The Cordilleran Mountain Belt of North America is one of the world's classic foreland fold and thrust belts. The Sevier Belt represents the thin skinned front of this orogeny, consisting of thrust faults and folds that shortened and transported sequences of Devonian to Cretaceous strata eastward. There is a general increase in deformation westwards which provides an ideal geological setting to explore the potential link between Anisotropy of Magnetic Susceptibility (AMS) and results from clast based strain analyses of sandstones. Studies attempting to define the relationship between AMS and finite strain have been in vogue since the link between layer parallel shortening and AMS was first established. The understanding of this relationship, despite proven strong correlations between the AMS tensors and tectonic directions, is complicated by competing sub-fabrics, as well as the various magnetic properties of the minerals contributing to the AMS fabric. It has become very clear that the sensitivity of AMS is capable of detecting incipient tectonic fabrics, <5% shortening, which is typically outside the range of most dedicated strain analyses. Despite this, there has been little published research into the relationship between the classic fabric analysis techniques or strain estimate methods and AMS. This may be due to the particularly laborious task of calculating strain estimates using large marker populations and/or the pitfalls of trying to relate the magnitudes of the magnetic ellipse to the magnitudes of the strain ellipse. Rather than trying to estimate finite strain directly from the AMS ellipsoid, we are using the ability of AMS to accurately and quickly qualify the petrofabric and determine the origin of that fabric (i.e., whether it is purely sedimentary, composite bedding/tectonic or dominantly tectonic etc.). Where as most methods of estimating strain have poor accuracy constraints in low strain regimes. In an attempt to account for this AMS is being utilised as a control for the sensitivity limits for these strain analysis methods, this is in part due to low sample counts, subjectivity and human error. This research uses recently developed and remodified strain analysis methods that are being utilised in a Mathematica environment. The use of Mathematica allows for objective, swift, reproducible and automated calculation of a suite of strain estimate techniques, such as the Fry method, a variation of Ramsay's Nearest Neighbour Method and the Mean Radial Length Method.

Mc Carthy, Dave; Meere, Pat; Petronis, Mike; Mulchrone, Kieran

2013-04-01

75

The Lower Sevier River Basin Crop Monitor and Forecast Decision Support System: Exploiting Landsat Imagery to Provide Continuous Information to Farmers and Water Managers  

NASA Astrophysics Data System (ADS)

The last century has seen a large number of innovations in agriculture such as better policies for water control and management, upgraded water conveyance, irrigation, distribution, and monitoring systems, and better weather forecasting products. In spite of this, irrigation management and irrigation water deliveries by farmers/water managers is still based on factors like water share amounts, tradition, and past experience on irrigation. These factors are not necessarily related to the actual crop water use; they are followed because of the absence of related information provided in a timely manner at an affordable cost. Thus, it is necessary to develop means to deliver continuous and personalized information about crop water requirements to water users/managers at the field and irrigation system levels so managers at these levels can better quantify the required versus available water for irrigation during the irrigation season. This study presents a new decision support system (DSS) platform that addresses the absence of information on actual crop water requirements and crop performance by providing continuous updated farm-based crop water use along with other farm performance indicators such as crop yield and farm management to irrigators and water managers. This DSS exploits the periodicity of the Landsat Satellite Mission (8 to 16 days, depending on the period of interest) to provide remote monitoring at the individual field and irrigation system levels. The Landsat satellite images are converted into information about crop water use, yield performance and field management through application of state-of-the-art semi-physical and statistical algorithms that provide this information at a pixel basis that are ultimately aggregated to field and irrigation system levels. A version of the DSS has been implemented for the agricultural lands in the Lower Sevier River, Utah, and has been operational since the beginning of the 2013 irrigation season. The main goal of this DSS implementation is to provide continuous and personalized information to farmers and water managers regarding crops in fields and the irrigation delivery system throughout the irrigation season so that decisions related to agricultural water use can result in water savings while not diminishing crop yields.

Torres-Rua, A. F.; Walker, W. R.; McKee, M.

2013-12-01

76

Counties with Established Counties without Established  

E-print Network

Counties with Established Burn Bans Counties without Established Burn Bans Outdoor Burn Bans For More Information Please Contact Your Local County Judge's Office or County Website Email updates to: burnban@tfs.tamu.edu Link to see if your county is under a RED FLAG WARNING

77

Low-temperature thermochronology of the Laramide Ranges and eastward translation of shortening in the Sevier Belt, Wyoming, Utah and Montana  

NASA Astrophysics Data System (ADS)

This dissertation contains two studies that use very different techniques to investigate the Mesozoic and Cenozoic tectonics of the western USA. The first study investigates shortening in the Sevier thrust belt of northeast Utah and southwest Wyoming using cross sections and seismic reflection data. The second study investigates the low-temperature thermochronology of the Laramide Ranges using apatite (U-Th)/He dating. We used cross sections and seismic reflection data to investigate bed length discrepancies within the hanging wall of the Absaroka thrust in the Sevier thrust belt of northeast Utah and southwest Wyoming. Restoration of cross sections suggests that there was 8-14 km of pre-Absaroka-thrust shortening above the Jurassic Preuss salt detachment, but not below it, in the hanging wall of the Absaroka thrust. Reflection seismic data over the hanging wall of the Crawford thrust show that the Crawford thrust is not offset along the Preuss salt detachment, indicating that the additional shortening on the Absaroka plate was transferred east before main movement on the Crawford thrust. Although early displacement on the Crawford thrust cannot be ruled out as the cause of the extra shortening, surface and subsurface geology suggests slip from the western thrust system (Willard and Lost Creek thrusts) was transferred several tens of kilometers east along the Jurassic Preuss salt detachment between 102-90 Ma, to the future location of the Absaroka thrust hanging wall. The lack of deformation of the Crawford thrust on the seismic data, along with shortening and extension estimates from cross sections, also indicate that the magnitude of Paleocene and post-early Eocene shortening on the Medicine Butte thrust was essentially offset by subsequent extension on the middle Eocene to late Oligocene Almy-Acocks normal-fault system. For the second study in this dissertation, we dated 91 borehole and surface samples from Laramide-age, basement-cored uplifts of the Rocky Mountain foreland (Wind River, Beartooth, Bighorn and Laramie Ranges) and the Uncompahgre Uplift using the apatite (U-Th)/He system. Apatite (U-Th)/He ages generally decrease with increasing subsurface depth (decreasing elevation) and most samples show age dispersion ranging from tens to hundreds of Myr. Additionally, several samples show correlations between apatite (U-Th)/He age and effective U concentration (eU = [U] + 0.235[Th]) of the crystal, indicating that radiation damage has affected He diffusivity, and hence (U-Th)/ He age. Many surface and near-surface samples have apatite (U-Th)/He ages that are older than corresponding apatite fission-track ages. Forward modeling of Laramide-type thermal histories using a radiation damage diffusion model showed that (U-Th)/He ages may be widely dispersed, and may be older than corresponding apatite fission-track ages within a fossil He partial retention zone. Most of our samples, however, do not show the correlation between (U-Th)/He age and eU predicted by radiation damage diffusion models. We investigated the influence of both grain size and eU content and show that the effects of grain size can obscure (U-Th)/ He age-eU correlations and, similarly, the effect of eU variation can obscure (U-Th)/ He age-grain size correlations. (U-Th)/He ages that are older than fission-track ages from high peaks in the Wind River Range, and from some samples from the Beartooth Range, are most likely the result of He implantation from high eU phases. Best-fit thermal histories from the inversion of age-eU pairs were extrapolated to other elevations to create model age-elevation profiles for a range of eU concentrations. These model profiles approximate our real data. Inverse modeling of (U-Th)/He age data suggests that rapid exhumation within the Laramide province likely began earlier in the Bighorn Mountains (before 71 Ma) than the Beartooth Range (before 58 Ma), and that the borehole at the northern end of the Laramie Range penetrated a fault sliver at depth.

Peyton, Sara Lynn

78

Nucleotide Sequence Analysis of Integrative Conjugative Element Tn5253 of Streptococcus pneumoniae  

PubMed Central

Conjugative transposon Tn5253, an integrative conjugative element (ICE) of Streptococcus pneumoniae carrying the cat and tet(M) genes, was shown to be 64,528 bp in size and to contain 79 open reading frames, of which only 38 could be annotated. Two distinct genetic elements were found integrated into Tn5253: Tn5251 (18,033 bp), of the Tn916-Tn1545 family of ICEs, and ?cat(pC194) (7,627 bp), which could not conjugate but was capable of intracellular mobility by excision, circularization, and integration by homologous recombination. The highest conjugation frequency of Tn5253 was observed when Streptococcus pyogenes was the donor (6.7 10?3 transconjugants/donor). PMID:24295984

Iannelli, Francesco; Santoro, Francesco; Oggioni, Marco R.

2014-01-01

79

8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. DETAIL SHOT OF POST AND RAIL AND CUT AWAY VIEW OF ABUTMENT SUPPORT INTO THE GROUND - Lost Creek Road Bridge, Spanning Sevier River at County Road 243, 2 miles east of Aurora, Aurora, Sevier County, UT

80

Nonlinear pressure dependence of TN in almost multiferroic EuTiO3.  

PubMed

The antiferromagnetic (AFM) phase transition temperature TN of EuTiO3 has been studied as a function of pressure p. The data reveal a nonlinear dependence of TN on p with TN increasing with increasing pressure. The exchange interactions exhibit an analogous dependence on p as TN (if the absolute value of the nearest neighbor interaction is considered) and there is evidence that the AFM transition is robust with increasing pressure. The corresponding Weiss temperature ?W remains anomalous since it always exhibits positive values. The data are analyzed within the Bloch power law model and provide excellent agreement with experiment. PMID:23963024

Guguchia, Z; Caslin, K; Kremer, R K; Keller, H; Shengelaya, A; Maisuradze, A; Bettis, J L; Khler, J; Bussmann-Holder, A; Whangbo, M-H

2013-09-18

81

Nonlinear pressure dependence of TN in almost multiferroic EuTiO3  

NASA Astrophysics Data System (ADS)

The antiferromagnetic (AFM) phase transition temperature TN of EuTiO3 has been studied as a function of pressure p. The data reveal a nonlinear dependence of TN on p with TN increasing with increasing pressure. The exchange interactions exhibit an analogous dependence on p as TN (if the absolute value of the nearest neighbor interaction is considered) and there is evidence that the AFM transition is robust with increasing pressure. The corresponding Weiss temperature ?W remains anomalous since it always exhibits positive values. The data are analyzed within the Bloch power law model and provide excellent agreement with experiment.

Guguchia, Z.; Caslin, K.; Kremer, R. K.; Keller, H.; Shengelaya, A.; Maisuradze, A.; Bettis, J. L., Jr.; Khler, J.; Bussmann-Holder, A.; Whangbo, M.-H.

2013-09-01

82

Diversity of Tn1546 Elements in Clinical Isolates of Glycopeptide-Resistant Enterococci from Scottish Hospitals  

PubMed Central

The Tn1546-related elements of 48 Van glycopetide-resistant enterococci were compared. Ten distinct Tn1546 types were identified with variation primarily due to IS1542 and IS1216V-like insertions. Clonal isolates frequently differed in their Tn1546 type, indicating instability of Tn1546-related elements. A putative hybrid promoter was identified, generated upstream of vanR by the insertion of IS1542. The presence of this hybrid promoter was associated with constitutive expression of the van genes and elevated teicoplanin resistance. PMID:11257055

Brown, A. R.; Townsley, A. C.; Amyes, S. G. B.

2001-01-01

83

Nucleotide sequences specific for Tn1545-like conjugative transposons in pneumococci and staphylococci resistant to tetracycline.  

PubMed Central

The distributions of tet(M) and conjugative transposons related to Tn1545 were studied by hybridization in 47 clinical isolates of Streptococcus pneumoniae resistant to tetracycline. Resistance to tetracycline was always associated with resistance to minocycline and was due to the presence of the tet(M) gene. Association of tet(M) with int-Tn, the gene encoding the protein required for the movements of Tn1545-like transposons, was found in all but one strain of S. pneumoniae. In contrast, int-Tn was detected in only 2 of 37 strains of Staphylococcus spp. harboring tet(M). Images PMID:1656870

Poyart-Salmeron, C; Trieu-Cuot, P; Carlier, C; Courvalin, P

1991-01-01

84

JANUARY 2007 MINNESOTA COUNTIES 5 helps counties  

E-print Network

JANUARY 2007 MINNESOTA COUNTIES 5 Extension workshop helps counties communicate their contributions to the common good By Laura Kalambokidis and Ryan Pesch County government service providers can readily provide services. While participants who directly benefit from county programs are a source of crucial political

Amin, S. Massoud

85

Washington County Kit Carson County Phillips County Yuma County Sedgwick County 181 Birch Ave 251 16th  

E-print Network

Washington County Kit Carson County Phillips County Yuma County Sedgwick County 181 Birch Ave 251://goldenplains.colostate.edu Colorado State University, U.S. Department of Agriculture and Kit Carson, Phillips, Sedgwick, Washington Edgar at 970-522-3200, extension 276. Golden Plains Area Washington County #12;

86

National Association of Counties  

NSDL National Science Digital Library

With 3066 U.S. counties, the National Association of Counties (NACo) has the arduous task of making sure the nations counties are acknowledged and understood in the halls of the White House and Congress. NACo, the only national government organization devoted to highlighting and improving the understanding of county issues, "collects, researches, publishes and disseminates a variety of different information for, on and about counties." On their Web site, viewers have access to a comprehensive and in-depth database of county information including county population; census bureau quick facts; elected county officials; county codes and ordinances; county policies; links to Capitolimpact.com, which provides nationwide county statistics such as economic and demographic data; and much more. This site is easily navigable, has counties arranged alphabetically by state, and would be of value to anyone living inside a county domain --- which is just about everyone.

2002-01-01

87

Networking Lab @ UniTN http://disi.unitn.it/networking  

E-print Network

and Computer Crime: Forensic Science, Computers, and the Internet, Second Edition," by Eoghan Casey @ UniTN http://disi.unitn.it/networking Parte 4:Test Case ed Esempi Computer Crimes e Phishing (C. FusiNetworking Lab @ UniTN http://disi.unitn.it/networking Parte 3: Computer Forensic Digital evidence

Granelli, Fabrizio

88

78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN  

Federal Register 2010, 2011, 2012, 2013

...Amendment of Class E Airspace; Tri-Cities, TN AGENCY: Federal Aviation Administration...amends Class E airspace for the Tri-Cities, TN area, by correcting the regulatory...the Class E surface airspace at Tri-Cities Regional Airport, Tri-Cities,...

2013-07-23

89

TN (TRADE NAFTA) STATUS Who and what types of position will qualify?  

E-print Network

to the two-year home residence requirement (HRR) of the J-1 visa. Obtaining TN status does not waive the requirement (the person would still be ineligible for H, L or permanent resident status until the HRR had been with the HRR. Although the TN must be renewed every three years and may not be for permanent employment

Scharer, John E.

90

Mapping of Escherichia Coli Chromosomal Tn5 and F Insertions by Pulsed Field Gel Electrophoresis  

PubMed Central

A low resolution Not I physical map of Escherichia coli was recently constructed. In this report we demonstrated that this map can be used to map Tn5 and F insertions physically. The transposon, Tn5, contains Not I recognition sequences in its IS50 sequences. F plasmid contains an unmapped Not I site. Hence, the location of Tn5 and F in the chromosome can be mapped by identifying the location of the introduced Not I sites using pulsed field gel electrophoresis. The physical mapping of genetically mapped Tn5 insertions confirm the previously constructed Not I map and helps align the E. coli physical and genetic maps. The use of Tn5 can assist the construction of both physical and genetic maps for microorganisms lacking such maps. Variations on this approach will facilitate physical mapping with a wide variety of organisms, enzymes, and genetic elements. PMID:2840334

Smith, C. L.; Kolodner, R. D.

1988-01-01

91

Adult teleost heart expresses two distinct troponin C paralogs: cardiac TnC and a novel and teleost-specific ssTnC in a chamber- and temperature-dependent manner.  

PubMed

The teleost-specific whole genome duplication created multiple copies of genes allowing for subfunctionalization of isoforms. In this study, we show that the teleost cardiac Ca2+-binding troponin C (TnC) is the product of two distinct genes: cardiac TnC (cTnC, TnnC1a) and a fish-specific slow skeletal TnC (ssTnC, TnnC1b). The ssTnC gene is novel to teleosts as mammals have a single gene commonly referred as cTnC but which is also expressed in slow skeletal muscle. In teleosts, the data strongly indicate that these are two TnC genes are different paralogs. Because we determined that ssTnC exists across many teleosts but not in basal ray-finned fish (e.g., bichir), we propose that these paralogs are the result of an ancestral tandem gene duplication persisting only in teleosts. Quantification of mRNA levels was used to demonstrate distinct expression localization patterns of the paralogs within the chambers of the heart. In the adult zebrafish acclimated at 28C, ssTnC mRNA levels are twofold greater than cTnC mRNA levels in the atrium, whereas cTnC mRNA was almost exclusively expressed in the ventricle. Meanwhile, rainbow trout acclimated at 5C showed cTnC mRNA levels in both chambers significantly greater than ssTnC. Distinct responses to temperature acclimation were also quantified in both adult zebrafish and rainbow trout, with mRNA in both chambers shifting to express higher levels of cTnC in 18C acclimated zebrafish and 5C acclimated trout. Possible subfunctionalization of TnC isoforms may provide insight into how teleosts achieve physiological versatility in chamber-specific contractile properties. PMID:23881286

Genge, Christine E; Davidson, William S; Tibbits, Glen F

2013-09-16

92

DNA damage differentially activates regional chromosomal loci for Tn7 transposition in Escherichia coli.  

PubMed

The bacterial transposon Tn7 recognizes replicating DNA as a target with a preference for the region where DNA replication terminates in the Escherichia coli chromosome. It was previously shown that DNA double-strand breaks in the chromosome stimulate Tn7 transposition where transposition events occur broadly around the point of the DNA break. We show that individual DNA breaks actually activate a series of small regional hotspots in the chromosome for Tn7 insertion. These hotspots are fixed and become active only when a DNA break occurs in the same region of the chromosome. We find that the distribution of insertions around the break is not explained by the exonuclease activity of RecBCD moving the position of the DNA break, and stimulation of Tn7 transposition is not dependent on RecBCD. We show that other forms of DNA damage, like exposure to UV light, mitomycin C, or phleomycin, also stimulate Tn7 transposition. However, inducing the SOS response does not stimulate transposition. Tn7 transposition is not dependent on any known specific pathway of replication fork reactivation as a means of recognizing DNA break repair. Our results are consistent with the idea that Tn7 recognizes DNA replication involved in DNA repair and reveals discrete regions of the chromosome that are differentially activated as transposition targets. PMID:18562643

Shi, Qiaojuan; Parks, Adam R; Potter, Benjamin D; Safir, Ilan J; Luo, Yun; Forster, Brian M; Peters, Joseph E

2008-07-01

93

Home Town: Greeneville, TN High School: Chuckey-Doak High School  

E-print Network

Top! When I was little I went to the women's basketball games with my mom and jammed out every time I, Running, Watching Teen Wolf and The Carrie Diaries, Hanging out at UTOP #12;Home Town: Nashville, TN High

Wang, Xiaorui "Ray"

94

77 FR 4007 - Grant of Authority for Subzone Status, Delta Faucet Company (Faucets), Jackson, TN  

Federal Register 2010, 2011, 2012, 2013

...Status, Delta Faucet Company (Faucets), Jackson, TN Pursuant to its authority under the...facility of Delta Faucet Company, in Jackson, Tennessee, (FTZ Docket 42-2010...facility of Delta Faucet Company, located in Jackson, Tennessee (Subzone 78I), as...

2012-01-26

95

Molecular Analysis of Tn1546 in Enterococcus faecium Isolated from Animals and Humans  

Microsoft Academic Search

The internal areas and the position of integration of the glycopeptide resistance element Tn1546 were char- acterized by using PCR fragment length polymorphism, sequencing, and DNA hybridization techniques with 38 high-level vancomycin-resistant Enterococcus faecium isolates of human and animal origins from Europe and the United States. Only minor variations in the coding regions within Tn1546 were found, suggesting high genetic

LARS BOG JENSEN; PETER AHRENS; LONE DONS; RONALD N. JONES; ANETTE M. HAMMERUM; FRANK MLLER AARESTRUP

96

Further characterization of the binding properties of two monoclonal antibodies recognizing human Tn red blood cells  

Microsoft Academic Search

Summary The terminal a anomeric GalNAc residue is an essential sugar for the Tn glycotope, human blood group A determinant, and Forssman antigen. In a previous study [King M.J., Parson S.F., Wu A.M., Jones N., Transfusion 31: 142149, 1991], we defined two monoclonal antibodies (MoAbs, BRIC66 and BRIC111) reacting with human Tn red blood cells. However, more advanced studies of

Albert M. Wua; June H. Wub; Hsiang-Wei Kuoa; Anthony Herpa

2005-01-01

97

Chromosomal gentamicin resistance transposon Tn3706 in Streptococcus agalactiae B128.  

PubMed Central

Streptococcus agalactiae B128 is the only highly gentamicin-resistant group B streptococcal (GBS) strain described so far. This strain carries a chromosomal gentamicin resistance transposon, designated Tn3706, which is similar, if not identical, to the Tn4001 and Tn5281 transpons detected in Staphylococcus aureus and Enterococcus faecalis, respectively. Transposition of Tn3706 occurred onto the GBS plasmid pIP501 in two different loci of its 7.5-kb AvaII fragment carrying the genes for chloramphenicol and erythromycin resistance. Molecular analysis of pIP501 derivatives showed that Tn3706 is composed of a central fragment containing the aac6'-aph2" gene; this fragment is flanked by two tandemly repeated copies of IS256 at the 5' extremity of the resistance gene and a single inverted copy of IS256 at its 3' extremity. The two tandemly repeated copies of IS256 were separated by a 6-bp segment identical to that found, in the same orientation, in the IS256-aac6'-aph2" junction. The hybrid replicons pIP501::Tn3706 were found to be structurally unstable following conjugative transfer between GBS strains. Numerous individual copies of IS256 were detected in B128, but this insertion sequence was not found in the 11 wild-type, gentamicin-susceptible GBS strains studied. PMID:8723445

Horaud, T; de Cespedes, G; Trieu-Cuot, P

1996-01-01

98

A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2.  

PubMed

We report the characterization of a DNA primase/polymerase protein (PolpTN2) encoded by the pTN2 plasmid from Thermococcus nautilus. Sequence analysis revealed that this protein corresponds to a fusion between an N-terminal domain homologous to the small catalytic subunit PriS of heterodimeric archaeal and eukaryotic primases (AEP) and a C-terminal domain related to their large regulatory subunit PriL. This unique domain configuration is not found in other virus- and plasmid-encoded primases in which PriS-like domains are typically fused to different types of helicases. PolpTN2 exhibited primase, polymerase and nucleotidyl transferase activities and specifically incorporates dNTPs, to the exclusion of rNTPs. PolpTN2 could efficiently prime DNA synthesis by the T. nautilus PolB DNA polymerase, suggesting that it is used in vivo as a primase for pTN2 plasmid replication. The N-terminal PriS-like domain of PolpTN2 exhibited all activities of the full-length enzyme but was much less efficient in priming cellular DNA polymerases. Surprisingly, the N-terminal domain possesses reverse transcriptase activity. We speculate that this activity could reflect an ancestral function of AEP proteins in the transition from the RNA to the DNA world. PMID:24445805

Gill, Sukhvinder; Krupovic, Mart; Desnoues, Nicole; Bguin, Pierre; Sezonov, Guennadi; Forterre, Patrick

2014-04-01

99

A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2  

PubMed Central

We report the characterization of a DNA primase/polymerase protein (PolpTN2) encoded by the pTN2 plasmid from Thermococcus nautilus. Sequence analysis revealed that this protein corresponds to a fusion between an N-terminal domain homologous to the small catalytic subunit PriS of heterodimeric archaeal and eukaryotic primases (AEP) and a C-terminal domain related to their large regulatory subunit PriL. This unique domain configuration is not found in other virus- and plasmid-encoded primases in which PriS-like domains are typically fused to different types of helicases. PolpTN2 exhibited primase, polymerase and nucleotidyl transferase activities and specifically incorporates dNTPs, to the exclusion of rNTPs. PolpTN2 could efficiently prime DNA synthesis by the T. nautilus PolB DNA polymerase, suggesting that it is used in vivo as a primase for pTN2 plasmid replication. The N-terminal PriS-like domain of PolpTN2 exhibited all activities of the full-length enzyme but was much less efficient in priming cellular DNA polymerases. Surprisingly, the N-terminal domain possesses reverse transcriptase activity. We speculate that this activity could reflect an ancestral function of AEP proteins in the transition from the RNA to the DNA world. PMID:24445805

Gill, Sukhvinder; Krupovic, Mart; Desnoues, Nicole; Beguin, Pierre; Sezonov, Guennadi; Forterre, Patrick

2014-01-01

100

Variation on a theme; an overview of the Tn916/Tn1545 family of mobile genetic elements in the oral and nasopharyngeal streptococci  

PubMed Central

The oral and nasopharyngeal streptococci are a major part of the normal microbiota in humans. Most human associated streptococci are considered commensals, however, a small number of them are pathogenic, causing a wide range of diseases including oral infections such as dental caries and periodontitis and diseases at other body sites including sinusitis and endocarditis, and in the case of Streptococcus pneumoniae, meningitis. Both phenotypic and sequence based studies have shown that the human associated streptococci from the mouth and nasopharynx harbor a large number of antibiotic resistance genes and these are often located on mobile genetic elements (MGEs) known as conjugative transposons or integrative and conjugative elements of the Tn916/Tn1545 family. These MGEs are responsible for the spread of the resistance genes between streptococci and also between streptococci and other bacteria. In this review we describe the resistances conferred by, and the genetic variations between the many different Tn916-like elements found in recent studies of oral and nasopharyngeal streptococci and show that Tn916-like elements are important mediators of antibiotic resistance genes within this genus. We will also discuss the role of the oral environment and how this is conducive to the transfer of these elements and discuss the contribution of both transformation and conjugation on the transfer and evolution of these elements in different streptococci. PMID:25368607

Santoro, Francesco; Vianna, Morgana E.; Roberts, Adam P.

2014-01-01

101

78 FR 60375 - Norfolk Southern Railway Company-Discontinuance of Service Exemption-in Claiborne County, TN, and...  

Federal Register 2010, 2011, 2012, 2013

...the Board should be sent to applicants' representatives: Louis E. Gitomer, 600 Baltimore Ave., Suite 301, Towson, MD...Decided: September 26, 2013. By the Board, Richard Armstrong, Acting Director, Office of Proceedings. Jeffrey...

2013-10-01

102

Regional Lead Agents and County Coordinators 2011 RESPONSIBILITY NAME COUNTY  

E-print Network

#12;Regional Lead Agents and County Coordinators 2011 RESPONSIBILITY NAME COUNTY REGION 1 Regional Lead Millie Davenport HGIC County Coordinator Matt Burns Pickens County Coordinator Marty Watt Anderson County Coordinator Morris Warner Oconee REGION 2 Regional Lead Danny Howard Greenville County Coordinator

Bolding, M. Chad

103

76 FR 58241 - Opportunity for Designation in the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA...  

Federal Register 2010, 2011, 2012, 2013

...Iowa Grain Inspection Service, Inc.'s, area); and West Bend Elevator Co., Algona, Kossuth County; Stateline Coop., Burt, Kossuth County; Gold-Eagle, Goldfield, Wright County; and North Central Coop, Holmes, Wright County (located...

2011-09-20

104

Balance : Lancaster County's tragedy  

E-print Network

Lancaster County, Pennsylvania residents are proud of their agricultural heritage. They do not want to see their farmland disappear. But the County continues to be developed into residential subdivisions. This thesis ...

Gingrich, Valerie (Valerie J.)

2007-01-01

105

County by County in Ohio Genealogy. Revised.  

National Technical Information Service (NTIS)

The State Library of Ohio's genealogy collection of over 8,000 items is listed by county. Within each county listing the sources are designated as atlases, cemetery and death records, census records (the majority from the 1800's), family-church-Bible reco...

P. Khouw

1978-01-01

106

Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance.  

PubMed Central

Two different enteric plasmids of widely separate origins were observed to carry a new 15.3-kb trimethoprim resistance transposon, Tn5086, also mediating resistance to mercuric ions and to a low level of sulfonamide. The trimethoprim resistance gene characterized from Tn5086 was found to be distinct from those found earlier and was designated type VII. Molecular analysis demonstrated that Tn5086 is closely related to Tn21. The internal part of Tn21 and Tn5086, the element referred to as the integron, was found to be different. First, the integron of Tn5086 contains a 0.62-kb cassette formed by the trimethoprim resistance gene dhfrVII and its immediate surroundings instead of the 0.86-kb aadA1 cassette of Tn21. Second, the integron of Tn5086 lacks a 4.2-kb segment 3' of sulI in Tn21. The dhfrVII gene commences with a UUG codon but was otherwise seen to be markedly related to the cassette genes dhfrI, dhfrV, and dhfrVI. The four related dihydrofolate reductases of 157 amino acids encoded by these genes contain a glutamate instead of the aspartic acid residue found at position 27 of the active center of the chromosomal enzyme from Escherichia coli. PMID:8383666

Sundstrm, L; Swedberg, G; Skld, O

1993-01-01

107

Tn5 transposase and tagmentation procedures for massively scaled sequencing projects  

PubMed Central

Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications. PMID:25079858

Picelli, Simone; Bjrklund, sa K.; Reinius, Bjrn; Sagasser, Sven; Winberg, Gsta

2014-01-01

108

Distribution of the catabolic transposon Tn5271 in a groundwater bioremediation system.  

PubMed Central

The distribution of Tn5271-related DNA sequences in samples of groundwater and a groundwater bioremediation system at the Hyde Park (Niagara Falls, N.Y.) chemical landfill site was investigated. PCR amplification of target sequences within the cha genes of Tn5271 revealed similar sequences in the groundwater community and in samples from the sequencing batch reactors treating that groundwater. Cell dilution combined with PCR amplification indicated that cha sequences were carried in about 1 of 10 culturable bacteria from the treatment system. Characterization of isolates involved in chlorobenzoate and toluene biodegradation in the treatment system indicated that two phenotypic clusters, Alcaligenes faecalis type 2 and CDC group IVC-2, contained all of the Tn5271 probe-positive isolates from the community. These two groups differed phenotypically from recipient groups isolated following horizontal transfer of pBRC60 (Tn5271) in pristine freshwater microcosms. A genetic rearrangement in Tn5271 attributable to the intramolecular transposition of the flanking element IS1071R was detected in an isolate from the treatment system. Comparison of the structure of the intramolecular transposition derivative from groundwater isolate OCC13(pBRC13) with a laboratory-derived intramolecular transposition derivative of pBRC60 revealed similarities. The rearrangement was shown to increase the stability of the plasmid under starvation conditions. Images PMID:8117095

Wyndham, R C; Nakatsu, C; Peel, M; Cashore, A; Ng, J; Szilagyi, F

1994-01-01

109

The SF1 helicase encoded by the archaeal plasmid pTN2 of Thermococcus nautili.  

PubMed

We expressed, purified, and characterized the helicase encoded by ORF1 of the Thermococcus nautili pTN2 plasmid (Soler et al. Nucl Acids Res 38, 5088-5104, 2010). The enzyme, which belongs to the SF1 family of helicases, possesses NTPase activity, with a strong preference for ATP and GTP as compared to CTP and TTP; dATP was also a substrate. Triphosphatase activity was strongly stimulated by single-stranded DNA and, to a lesser extent, by double-stranded DNA. Unwinding of duplexes comprising a fluorescent oligonucleotide was monitored by fluorescence polarization spectroscopy and by polyacrylamide gel electrophoresis. As observed for enzymes of the same family, pTN2 helicase displays a strong preference for duplexes comprising a 3' single-stranded extension and proceeds from the 3' to the 5' end of the loading strand. Under the conditions of the in vitro assay, pTN2 helicase did not appear to be recycled, but stayed bound to single-stranded DNA, which explains why high concentrations of enzyme are required to unwind long stretches of duplex DNA. The helicase enhances the synthesis of double-stranded DNA by pTN2 primase and by T. nautili PolB polymerase primed by pTN2 primase but it did not enhance synthesis by Taq DNA polymerase. PMID:24889120

Bguin, Pierre; Baron, Bruno; Gill, Sukhvinder; Charpin, Nicole; Forterre, Patrick

2014-07-01

110

Conjugative transfer of Tn916 in Enterococcus faecalis: trans activation of homologous transposons.  

PubMed Central

Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harboring Tn916 delta E (a derivative in which erm is substituted for tet) on the chromosome and Tn916 on a nonconjugative plasmid. When these strains were used as donors, the two transposons were able to transfer independently; however, they were found to transfer and become coestablished in the recipient up to 50% of the time. In contrast, cotransfer of a plasmid marker located outside the transposon occurred at a frequency of no greater than 0.5%. Separate experiments showed that mobilization of the nonconjugative plasmids pAM401 and pVA749 by chromosome-borne copies of Tn916 occurred only at low frequencies (generally less than 2% cotransfer). The data imply that the initiation of transposition of Tn916 results in a trans activation that is specific for homologous transposons present in the same cell. PMID:1657880

Flannagan, S E; Clewell, D B

1991-01-01

111

Washington County Comprehensive plan  

E-print Network

Washington County Comprehensive plan October 2010 #12;This page is intentionally blank. #12;Table of ConTenTs #12;ii Washington County Comprehensive plan · 2010 #12;iii Table of ConTenTs Table of Con Washington County Comprehensive plan · 2010 4. makIng The Case for PlannIng 17 Collective vs. Individual

112

Percent Uninsured by County  

NSDL National Science Digital Library

This is a county by county visualization of the percentage of residents that are uninsured. The data are from a set available here: http://www-958.ibm.com/software/data/cognos/manyeyes/datasets/percent-uninsured-by-county/versions/1

Manyeyes

113

Persistent Tn polyagglutination syndrome during febrile neutropenia: a case report and review of the literature  

PubMed Central

Introduction Tn polyagglutination syndrome is a rare disorder that has been reported on only a few occasions in the literature, and, to the best of our knowledge, never before in the context of febrile neutropenia. Case presentation We report the case of a 26-year-old Caucasian woman who presented to our emergency department complaining of a persistent fever over the previous three days. She had a history of long-standing refractory pancytopenia with multi-lineage dysplasia and severe neutropenia, but she had rarely experienced infection. The results of a physical examination and multiple laboratory tests were unremarkable. While investigating the possible causes of the refractory, long-standing pancytopenia, the possibility of a polyagglutinable state was suggested. Blood samples were sent to the laboratory for an analysis of mixed-field seed lectin agglutination assay. A serum lectin panel confirmed the final diagnosis of Tn-activation. Conclusions We should include Tn-activation in our differential whenever we encounter cases of refractory long-standing idiopathic cytopenias and inconclusive bone marrow results displaying multi-lineage dysplasia. Novel genetic techniques have recently revealed the interesting pathophysiology of this phenomenon. The recognition and inclusion of Tn polyagglutination syndrome in our differential diagnoses has important clinical implications, given its main associated features, such as severe thrombocytopenia and neutropenia, which are usually linked to a benign clinical course and prognosis. Increased awareness of the polyagglutinable disorders will potentially decrease the need for invasive and costly medical interventions and also raises the need for monitoring of this specific sub-set of patients. In addition, the study of the expression and implications of Tn, and other similar antigens, offers a fascinating perspective for the study of its role in the diagnosis, prognosis and immunotherapy of solid tumors and hematological malignancies. The infrequency with which Tn polyagglutination syndrome is encountered, its clinical features and its pathophysiology make it a formidable diagnostic challenge. PMID:21235740

2011-01-01

114

Behavior and Target Site Selection of Conjugative Transposon Tn916 in Two Different Strains of Toxigenic Clostridium difficile  

PubMed Central

The insertion sites of the conjugative transposon Tn916 in the anaerobic pathogen Clostridium difficile were determined using Illumina Solexa high-throughput DNA sequencing of Tn916 insertion libraries in two different clinical isolates: 630?E, an erythromycin-sensitive derivative of 630 (ribotype 012), and the ribotype 027 isolate R20291, which was responsible for a severe outbreak of C. difficile disease. A consensus 15-bp Tn916 insertion sequence was identified which was similar in both strains, although an extended consensus sequence was observed in R20291. A search of the C. difficile 630 genome showed that the Tn916 insertion motif was present 100,987 times, with approximately 63,000 of these motifs located in genes and 35,000 in intergenic regions. To test the usefulness of Tn916 as a mutagen, a functional screen allowed the isolation of a mutant. This mutant contained Tn916 inserted into a gene involved in flagellar biosynthesis. PMID:22267673

Williams, Rachel; Langridge, Gemma C.; Turner, Daniel J.; Whalan, Rachael; Clayton, Chris; Lawley, Trevor; Hussain, Haitham; McCurrie, Katherine; Morden, Nicky; Allan, Elaine; Roberts, Adam P.

2012-01-01

115

Wetlands Regulatory Assistance Program ERDC TN-WRAP-12-1 August 2012  

E-print Network

Wetlands Regulatory Assistance Program ERDC TN-WRAP-12-1 August 2012 Approved for public release; distribution is unlimited. Updating Regional Supplements to the Corps of Engineers Wetland Delineation Manual by Jacob F. Berkowitz PURPOSE: Regional supplements to the Corps of Engineers Wetland Delineation Manual

US Army Corps of Engineers

116

Robyn M. Cox, PhD Professor ofAudiology, University ofMemphis, Memphis, TN  

E-print Network

Robyn M. Cox, PhD Professor ofAudiology, University ofMemphis, Memphis, TN ABSTRACT: Hearing Aids and the Loudness problem Perhaps the lnost ubiquitous problem encountered in hearing aid fitting .is a lack of satisfaction with loudness continues to be a significant and substantial problem for hearing aid wearers

Dasgupta, Dipankar

117

ERDC/EL TN-10-1 Effects of a Nonnative, Invasive Lovegrass  

E-print Network

ERDC/EL TN-10-1 May 2010 Effects of a Nonnative, Invasive Lovegrass on Agave palmeri Distribution lovegrass (Eragrostis lehmanniana) on the distribution and abundance of Palmer's agave (Agave palmeri reproductive efficiency. The genus Agave is an important native taxa to assess the effects of invasive grasses

US Army Corps of Engineers

118

ERDC/EL TN-10-4 Release of the Biological Control Agent  

E-print Network

starthistle, Centaurea solstitialis L., at Fort Hunter Liggett, an Army training installation in CaliforniaERDC/EL TN-10-4 July 2010 Release of the Biological Control Agent Puccinia jaceae var. solstitialis fungus, Puccinia jaceae var. solstitialis, as a biological control agent for management of yellow

US Army Corps of Engineers

119

Characterization of glucansucrase and dextran from Weissella sp. TN610 with potential as safe food additives.  

PubMed

Pear-derived Weissella sp. TN610 produced extracellular glycosyltransferase activity responsible for the synthesis of soluble exopolysaccharide from sucrose. Acid and dextranase-catalyzed hydrolysis revealed that the synthesized polymer was a glucan. According to (1)H and (13)C NMR analysis, the glucan produced by TN610 was a linear dextran made of 96% ?-(1?6) and 4% ?-(1?3) linkages. Zymogram analysis confirmed the presence of a unique glucansucrase of approximately 180 kDa in the cell-free supernatant from TN610. The crude enzyme, optimally active at 37C and pH 5, has promising potential for application as a food additive since it catalyzes dextran synthesis in sucrose-supplemented milk, allowing its solidification. A 4257-bp product corresponding to the mature glucansucrase gene was amplified by PCR from TN610. It encoded a polypeptide of 1418 residues having a calculated molecular mass of 156.089 kDa and exhibiting 96% and 95% identity with glucansucrases from Lactobacillus fermentum Kg3 and Weissella cibaria CMU, respectively. PMID:23046923

Bejar, Wacim; Gabriel, Valrie; Amari, Myriam; Morel, Sandrine; Mezghani, Monia; Maguin, Emmanuelle; Fontagn-Faucher, Catherine; Bejar, Samir; Chouayekh, Hichem

2013-01-01

120

Evoluzione della vegetazione nei pressi del Lago di Lavarone (TN) negli ultimi 2200 anni  

Microsoft Academic Search

RIASSUNTO - Evoluzione della vegetazione nei pressi del Lago di Lavarone (TN) negli ultimi 2200 anni - Lo studio pollinico e paleolimnologico della sequenza S5 proveniente dal Lago di Lavarone, condotto nel corso del progetto OLOAMBIENT, ha permesso di ottenere un quadro dettagliato sull'evoluzione del territorio nei pressi del lago negli ultimi 2200 anni. Sulla base delle informazioni archeologiche e

Enrico ARPENTI; Maria Letizia FILIPPI

121

ERDC/TN APCRP-EA-22 Management of Half Moon Lake,  

E-print Network

ERDC/TN APCRP-EA-22 May 2010 Management of Half Moon Lake, Wisconsin, for Improved Native Submersed that result in poor light penetration and limited colonizable macrophyte habitat. Half Moon Lake Half Moon Lake is shallow and supports a native submersed macrophyte community, poor underwater light

US Army Corps of Engineers

122

78 FR 6257 - Proposed Amendment of Class C Airspace; Nashville International Airport, TN  

Federal Register 2010, 2011, 2012, 2013

...RIN 2120-AA66 Proposed Amendment of Class C Airspace; Nashville International Airport...Nashville International Airport, TN, Class C airspace area by removing a cutout from the...proposing this action to return the Class C airspace area to the standard...

2013-01-30

123

Interactions of Protein Complexes on Supercoiled DNA: The Mechanism of Selective Synapsis by Tn 3 Resolvase  

Microsoft Academic Search

Looping interactions of distant sites on DNA molecules, mediated by DNA-binding proteins, feature in many regulated genetic processes. We used plasmids containing up to six res recombination sites for Tn3 resolvase to analyse looping interactions (synapsis) in this system. We observed that in plasmids with four or more res sites, certain pairs of sites recombine faster than others. The relative

J. Lesley Brown; Jiuya He; David J. Sherratt; W. Marshall Stark; Martin R. Boocock

2002-01-01

124

ERDC/TN APCRP-EA-24 Comparison of Three Biomass Sampling  

E-print Network

ERDC/TN APCRP-EA-24 July 2010 Comparison of Three Biomass Sampling Techniques on Submersed Aquatic R. Spickard2 PURPOSE: Quantifying biomass to measure aquatic plant abundance can be costly and labor intensive. This technical note compares several alternate, less exhaustive techniques for biomass sampling

US Army Corps of Engineers

125

ERDC/TN APCRP-EA-23 Effects of Nutrients, Salinity, and pH on  

E-print Network

ERDC/TN APCRP-EA-23 July 2010 Effects of Nutrients, Salinity, and pH on Salvinia molesta (Mitchell (nitrogen and phosphorus) concentra- tions, pH, and salinity on giant salvinia growth. BACKGROUND: Giant factors including temperature, nutrient availability, salinity, and pH may limit the distribution

US Army Corps of Engineers

126

Role of Myofibril-Inducing RNA in cardiac TnT expression in developing Mexican axolotl  

PubMed Central

The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene c, for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts.. Myofibril-Inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac Troponin-T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart PMID:17408593

Sferrazza, Gian-Franco; Zhang, Chi; Jia, Pingping; Lemanski, Sharon L.; Athauda, Gagani; Stassi, Alyssa; Halager, Kristine; Maier, Jennifer A.; Rueda-de-Leon, Elena; Gupta, Amit; Dube, Syamalima; Huang, Xupei; Prentice, Howard M.; Dube, Dipak K.; Lemanski, Larry F.

2007-01-01

127

Characterisation of Tn501, a transposon determining resistance to mercuric ions  

Microsoft Academic Search

The transposon encoding resistance to mercuric ions, Tn501, is 5.2 (0.1)106 daltons and is bounded by small inverted repeats. The restriction sites for the restriction endonucleases EcoRI, HindIII and SalGI have been mapped on the element.

P. M. Bennett; J. Grinsted; C. L. Choi; M. H. Richmond

1978-01-01

128

Technical Note PR-TN-2004/00370 Issued: 05/2004  

E-print Network

Technical Note PR-TN-2004/00370 Issued: 05/2004 Mass Customization in Ambient Narratives Mark van: Mass Customization in Ambient Narratives Author(s): M.G.L.M. van Doorn; A.P. de Vries Reviewers to the presence of a digital environment that is sensitive, adaptive and responsive to the presence of people

de Vries, Arjen P.

129

Submarine Ring of Fire 2003 Mariana Arc R/V T. G. Thompson Cruise TN-153  

E-print Network

#12;i Submarine Ring of Fire 2003 ­ Mariana Arc R/V T. G. Thompson Cruise TN-153 February 9 - March: Susan Merle, Robert Embley, Edward Baker and Bill Chadwick TABLE OF CONTENTS 1.0 Submarine Ring of Fire

130

Submarine Ring of Fire 2004 -Mariana Arc R/V Thomas G. Thompson, Cruise TN167  

E-print Network

#12;Submarine Ring of Fire 2004 - Mariana Arc R/V Thomas G. Thompson, Cruise TN167 March 27 - April..............................................................................................................................................3 1.0 SUBMARINE RING OF FIRE 2004 CRUISE SUMMARY (Bob Embley..........................................................................................................25 3.0 SUBMARINE RING OF FIRE (SROF) 2004 DISCIPLINE SUMMARIES

131

INTRODUCTION Tenascin-C (TN-C), a large glycoprotein of the extracellular  

E-print Network

it can bind chondroitin sulfate pro- teoglycans derived from smooth muscle culture (Chiquet and Fambrough chondroitin sulfate proteoglycan from brain, were shown to bind TN-C in vitro and co-localize with it in vivo cell mutants with a total block in heparan and chondroitin sulfate production failed to assemble

Erickson, Harold P.

132

Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome  

SciTech Connect

The gene for resistance to erythromycin and clindamycin, which is carried on the conjugative Bacteroides plasmid, pBF4, has been shown previously to be part of an element (Tn4351) that transposes in Escherichia coli. The authors have now introduced Tn4351 into Bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range IncP plasmid R751 (R751::Tn4351) and (ii) pSS-2, a chimeric plasmid which contains 33 kilobases of pBF4 (including Tn4351) cloned into the IncQ plasmid RSF1010 and which is mobilized by R751. When E. coli HB101, carrying either R751::Tn4351 or R751 and pSS-2, was mated with B. uniformis under aerobic conditions, Em/sup r/ transconjugants were detected at a frequency of 10 /sup -6/ to 10/sup -5/ (R751::Tn4351) or 10/sup -8/ to 10/sup -6/ (R751 and pSS-2). In matings involving pSS-2, all Em/sup r/ transconjugants contained simple insertions of Tn4351 in the chromosome, whereas in matings involving R751::Tn4351, about half of the Em/sup r/ transconjugants had R751 cointegrated with Tn4351 in the chromosome. Of the Em/sup r/ transconjugants, 13% were auxotrophs. Bacteroides spp. which had R751 cointegrated with Tn4351 in the chromosome did not transfer R751 or Tn4351 to E. coli HB101 or to isogenic B. uniformis, nor did the integrated R751 mobilize pE5-2, an E. coli-Bacteroides shuttle vector that contains a transfer origin that is recognized by R751.

Shoemaker, N.B.; Getty, C.; Gardner, J.F.; Salyers, A.A.

1986-03-01

133

FATE OF TN5 MUTANTS OF ROOT GROWTH-INHIBITING PSEUDOMONAS SP. IN INTACT SOIL-CORE MICROCOSMS  

EPA Science Inventory

Transposon Tn5 mutants of a wheat root growth-inhibiting nonfluorescent Pseudomonas sp. were inoculated into intact soil-core microcosms to determine the utility of intact soil cores for evaluating the fate and transport of microorganisms in agricultural ecosystems. ransposon Tn5...

134

County Staff or Area Staff?  

ERIC Educational Resources Information Center

McIntryre explores the questions of county-based extention and increased specialization in Indiana. He compares the multi-county with individual county systems using variables including clientele's reactions to the two systems. (NL)

McIntyre, William J.

1970-01-01

135

Too Many Counties?  

ERIC Educational Resources Information Center

Every state in the United States is divided into smaller units of local government. For example, Georgia has 159 counties. Proponents of reducing the number of counties argue for consolidation on the basis of efficiency and cost-effectiveness. Activities are suggested to help secondary students explore this issue. (RM)

Williams, Elmer D.

1985-01-01

136

Sheridan County Recreation Plan.  

ERIC Educational Resources Information Center

A pilot project investigating the feasibility of year-round recreational programs in rural counties of populations of less than 10,000 is described in this report. (Sheridan County, Kansas, was chosen as the project site.) Part I, the introductory section, briefly defines recreation and its relation to human needs. Part II provides a geographic

Webster, Elaine

137

Hancock County Awards Gala  

NASA Technical Reports Server (NTRS)

Gene Goldman (left), deputy director of NASA's John C. Stennis Space Center, accepts an Award of Excellence from Jack Zink, executive director of the Hancock County Port and Harbor Commission, during the 2008 Annual Hancock County Awards Gala. The Award of Excellence was presented to recognize Stennis Space Center's contribution to NASA's 50 years of excellence in space exploration.

2008-01-01

138

The Sugar Creek zinc deposit, Jackson Co. TN -- Exploration history, geology and mineralization  

Microsoft Academic Search

During the 60's and 70's zinc exploration of central TN and KY was active. The Sugar Creek Project was one of several investigated by Exxon. The discovery hole, Cu 15, was drilled in early 1973. The Sugar Creek Zinc Deposit was acquired by Independence Mining Co. in 1986 and I.M.C. has subsequently completed additional drilling, both stepout and confirmation holes.

G. Reinbold; A. V. Moran; D. L. Stevens

1993-01-01

139

Morphological and Functional Diversity of Ectomycorrhizal Fungi on Roan Mountain (NC\\/TN)  

Microsoft Academic Search

A comparison of ectomycorrhizal morphotypes and hypogeous fungi (truffles and false-truffles) in northern hardwood and spruce-fir forests on Roan Mountain (NC\\/TN) was performed to increase our knowledge of the fungal communities in the Southern Appalachian high elevation forests. These forests are home to an endangered subspecies and mycophagist, the Carolina northern flying squirrel (Glaucomys sabrinus coloratus), as well as the

Claire Bird; Coleman McCleneghan

2005-01-01

140

Genechip-detecting mutations in exon 8 in cTnI gene associated with FHCM  

NASA Astrophysics Data System (ADS)

As the rate of gene discovery accelerates, more efficient methods are needed to analyze genes in human tissues. Genechip, a kind of new device, is composed of DNA probes immobilized on a solid substrate. With the advantage of the high throughput information, genechip has become one of the best solutions to detect and analyse the mutations in genes. Hypertrophic cardiomyopathy (HCM), the most common cause of the sudden death in the young, is one of the diseases damaging people health most badly. It is an autosomal dominant disease. More than 55% of the HCM patients are genetic. The mutations of exon 8 in the Cardiac troponin I (cTnI) gene are closely associated with Family Hypertrophic Cardiomyopathy (FHCM). Our purpose is to perform the assay of the mutations in exon 8 in cTnI gene based on the genechip theory and technology. Special probes were designed to fabricate the genechip to detect the mutations in cTnI gene simultaneously. We designed two oligonucleotide sequences 5"-end labeled with fluorescein, one simulating wild-type and the other simulating mutant. We mixed oligonucleotide I and II together to simulate heterozygote. After optimizing the hybridization protocols, the fabricated genechip can detect the mutations in exon 8 in cTnI gene with relative high sensitivity and specificity. When applying the fabricated genechip to detect the target DNA sequence, we found that the fully complementary probe gave a fluorescent signal almost 50% stronger than that of the one base mismatched one, which is in accordance with the result from theoretic estimate. It is believed that an applicable special genechip can be developed for investigating and diagnosing FHCM after further improvement.

Zhang, Yuanying; He, Nongyue; Guo, Huishi; Yang, Di; Wan, Wenhui; Bian, Zhiping; Zhang, Jinan

2005-01-01

141

Algal flora of subalpine peat bog pools in the Krkonose Mts asy tn krkonosskch subalpnskch raselinis  

E-print Network

Algal flora of subalpine peat bog pools in the Krkonose Mts asy tní krkonosských subalpínských, Czech Repub- lic, e-mail: sylnova@natur.cuni.cz Nováková S. (2002): Algal flora of subalpine peat bog. The relationship be- tween algal flora and the environmental characteristics of the pools was studied. The p

142

Design analysis report for the TN-WHC cask and transportation system  

SciTech Connect

This document presents the evaluation of the Spent Nuclear Fuel Cask and Transportation System. The system design was developed by Transnuclear, Inc. and its team members NAC International, Nelson Manufacturing, Precision Components Corporation, and Numatec, Inc. The cask is designated the TN-WHC cask. This report describes the design features and presents preliminary analyses performed to size critical dimensions of the system while meeting the requirements of the performance specification.

Brisbin, S.A., Fluor Daniel Hanford

1997-02-13

143

Exchange bias in a ferromagnet/antiferromagnet system with TC<<TN  

NASA Astrophysics Data System (ADS)

This paper reports experimental results obtained on an unconventional exchange bias (EB) system where the ferromagnetic layer, Ni0.75Cu0.25, has lower ordering temperature (TC) than that (TN) of the antiferromagnetic one, NiO, with emphasis on modifying EB through either magnetic annealing or light-ion irradiation. Samples were cooled from temperatures higher than TN or in between TC and TN to room temperature with magnetic field applied in different in-plane directions. Upon ion irradiation, magnetic fields, parallel or antiparallel to the orientation of the field present during the films deposition, were applied to explore different effects on EB. We found that the EB direction can be completely reversed by means of either annealing or ion bombardment; however, both postdeposition treatments provide very little variation of the EB field value over that produced during the film's growth. The importance of the annealing field strength was also discussed. The results were interpreted based on a mechanism which assumes that the interfacial moments adjacent to the antiferromagnetic layer are responsible for establishing the exchange biasing in the paramagnetic state.

Sossmeier, K. D.; Pereira, L. G.; Schmidt, J. E.; Geshev, J.

2011-04-01

144

Alteration of surface properties in a Tn5 mutant strain of Rhizobium trifolii 0403.  

PubMed Central

A symbiotically defective mutant strain of Rhizobium trifolii, UR251, was obtained by transposon Tn5 mutagenesis of R. trifolii 0403 rif and recognized by its partially ineffective (Fix +/-) phenotype on white clover plants. UR251 had a single Tn5 insertion in plasmid DNA, a wild-type plasmid pattern, and no detectable Mu DNA sequences originally present in the vector used for Tn5 mutagenesis. Agglutination by the clover lectin trifoliin A and attachment to clover root hairs was higher with UR251 than with the wild-type strain. The capsular polysaccharide (CPS) of UR251 was altered, as shown by a slower rate of CPS depolymerization with a CPS beta-lyase, PD-I; more pyruvate and less acetate and 3-hydroxybutanoate noncarbohydrate substitutions as quantitated by 1H nuclear magnetic resonance; and a higher pyruvyl transferase activity (enzymatic pyruvylation of lipid-bound saccharides). The site of increased pyruvylation in the CPS of UR251 was on the terminal galactose of the branch of the repeating oligosaccharide unit. These results show that the level of noncarbohydrate substitutions of the CPS as well as pyruvyl transferase activity are altered in R. trifolii UR251 and that trifoliin A-binding ability and clover root hair attachment are improved in this mutant strain of R. trifolii 0403 rif. Images PMID:3029022

Gardiol, A E; Hollingsworth, R I; Dazzo, F B

1987-01-01

145

LEON COUNTY 4-H County Events 2012  

E-print Network

, friendly environment and to develop self confidence and poise. Participation also counts toward county'ers experience in the preparation and delivery of a show-and-tell presentation, with confidence in a public members Categories - Animal Science Individual/Family Resources Citizenship, Leadership, & Communication

Watson, Craig A.

146

Walton County Coastal Storm Damage Reduction Project Walton County, FL  

E-print Network

to reduce coastal storm damages by constructing berms and dunes along 18.8 miles of Walton County shorelineWalton County Coastal Storm Damage Reduction Project Walton County, FL 13 December 2012 ABSTRACT, several coastal storms have eroded the coastline of Walton County, resulting in recession

US Army Corps of Engineers

147

Clark county monitoring program  

SciTech Connect

Available in abstract form only. Full text of publication follows: Since 1988, Clark County has been one of the counties designated by the United States Department of Energy (DOE) as an 'Affected Unit of Local Government' (AULG). The AULG designation is an acknowledgement by the federal government that could be negatively impacted to a considerable degree by activities associated with the Yucca Mountain High Level Nuclear Waste Repository. These negative effects would have an impact on residents as individuals and the community as a whole. As an AULG, Clark County is authorized to identify 'any potential economic, social, public health and safety, and environmental impacts' of the potential repository (42 USC Section 10135(C)(1)(B)(1)). Toward this end, Clark County has conducted numerous studies of potential impacts, many of which are summarized in the Clark County's Impact Assessment Report that was submitted by the DOE and the president of the United States in February 2002. Given the unprecedented magnitude and duration of the DoE's proposal, as well as the many unanswered questions about the number of shipments and the modal mix, the estimate of impacts described in these studies are preliminary. In order to refine these estimates, Clark County Comprehensive Planning Department's Nuclear Waste Division is continuing to assess potential impacts. In addition, the County has implemented a Monitoring Program designed to capture changes to the social, environmental, and economic well-being of its residents resulting from the Yucca Mountain project and other significant events within the County. The Monitoring Program acts as an 'early warning system' that allows Clark County decision makers to proactive respond to impacts from the Yucca Mountain Project. (authors)

Conway, Sheila [Urban Environmental Research, 10100 W. Charleston Boulevard Las Vegas, 89135 (United States); Auger, Jeremy [Applied Analysis, 10100 West Charleston Blvd, Suite 200, Las Vegas, Nevada 89135 (United States); Navies, Irene [Clark County, Department of Comprehensive Planning, Las Vegas, NV (United States)

2007-07-01

148

76 FR 12280 - Revisions to the California State Implementation Plan, for Imperial County, Kern County, and...  

Federal Register 2010, 2011, 2012, 2013

...County, and Ventura County; Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District...

2011-03-07

149

76 FR 12306 - Revisions to the California State Implementation Plan, Imperial County, Kern County, and Ventura...  

Federal Register 2010, 2011, 2012, 2013

...County, and Ventura County; Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Kern County Air Pollution Control District...

2011-03-07

150

Wisconsin County Histories  

NSDL National Science Digital Library

Whether you're interested in Reedsburg, Rhinelander, or Rubicon, the Wisconsin County Histories website will not fail those keen on the history of the Badger State. Created by the Wisconsin Historical Society, this archive provides access to more than 80 standard histories of Wisconsin counties, most of which were published between 1850 and 1920. The majority of the volumes are over several hundred pages long, and they include detailed passages on cities within their respective counties, along with sketches of prominent leaders. Visitors can use the drop-down menu available on the homepage to find specific volumes, or they can also perform a full text search across all of the histories.

151

Pontotoc County Government Summer  

E-print Network

, disability, or status as a veteran in any of its policies, practices or procedures #12;Pontotoc County Zip Home Phone Cell Phone Email School Grade Completed Date Of Birth Parent/Guardian Home Phone Cell

Balasundaram, Balabhaskar "Baski"

152

76 FR 60802 - Foreign-Trade Zone 77-Memphis, TN; Withdrawal of Request for Temporary/Interim Manufacturing...  

Federal Register 2010, 2011, 2012, 2013

...Withdrawal of Request for Temporary/Interim Manufacturing Authority; Flextronics Logistics USA, Inc., (Cell Phone/Mobile Handset Kitting). Memphis, TN Notice is hereby given of the withdrawal of the application of the City of...

2011-09-30

153

The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17  

PubMed Central

The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; ?-D-GalpNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera. PMID:22297566

De Silva, Ravindra A.; Appulage, Dananjaya K.; Pietraszkiewicz, Halina; Bobbitt, Kevin R.; Media, Joe; Shaw, JiaJiu; Valeriote, Fred A.

2012-01-01

154

Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn552'phoA In Vitro Transposition System  

Microsoft Academic Search

Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogen- esis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552*phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the transposon inserts randomly into target DNA. M. tuberculosis genomic libraries were targeted with Tn552*phoA trans-

MIRIAM BRAUNSTEIN; THOMAS J. GRIFFIN; JORDAN I. KRIAKOV; SARAH T. FRIEDMAN; NIGEL D. F. GRINDLEY; WILLIAM R. JACOBS

2000-01-01

155

A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn 917 insertions  

Microsoft Academic Search

A rapid and general procedure has been devised for the pBR322-mediated cloning in Escherichia coli of Bacillus subtilis chromosomal DNA extending in a specified direction from any Tn917 insertion. Derivatives of Tn917 have been constructed that contain a pBR322-derived replicon, together with a chloramphenicol-resistance (Cmr) gene of Gram-positive origin (selectable in B. subtilis), inserted by ligation in two orientations into

Philip Youngman; John B. Perkins; Richard Losick

1984-01-01

156

Diversity of Tn1546Like Elements in Vancomycin-Resistant Enterococci Isolated from Humans and Poultry in Korea  

Microsoft Academic Search

To investigate the possible spread of vancomycin resistance among enterococci through horizontal gene transfer between two sources of vancomycin-resistant enterococci (VRE) isolated from farm animals and humans in Korea, molecular characterization of VRE isolated from poultry (20 isolates) and humans (35 isolates) was performed by PCR-restriction fragment length polymorphism typing of the vanA gene cluster (Tn1546). PCR mapping of Tn1546

Hak-Sun Yu; Sung-Yong Seol; Dong-Taek Cho

2003-01-01

157

Tn916-generated, lipooligosaccharide mutants of Neisseria meningitidis and Neisseria gonorrhoeae.  

PubMed Central

A library of Tn916-generated, tetracycline-resistant (Tc) mutants of the group B Neisseri meningitidis strain NMB was screened by using monoclonal antibodies (MAbs) that recognize structural differences in neisserial lipooligosaccharide (LOS). The LOS of parental strain NMB had a relative molecular mass of 4.5 kDa, reacted with MAbs 3F11 and 6B4 but not with MAb 4C4 or 6E4, and contained a lacto-N-neotetrose unit. Two phenotypically stable mutants, SS3 and R6, altered in LOS, were identified by colony immunoblots, electrophoresis, and Western immunoblots. The LOS of mutant SS3 was 3.4 kDa and reacted with MAbs 4C4 and 6E4 but not MAb 3E11 or 6B4. The LOS of mutant R6 was 3.1 to 3.2 kDa and reacted with MAb 6E4 but not MAb 3F11, 6B4, or 4C4. Thus, the LOSs of the R6 and SS3 mutants were predicted to contain different truncations of the core oligosaccharide. The LOS phenotype of each mutant was linked to Tc(r), as determined by transformation of the parent strain with DNA from the mutant. Southern hybridizations and single-specific-primer PCR revealed in each mutant a single truncated tn916 insertion which had lost genes required for mobilization. Tn916 mutagenesis was used to identify two distinct genetic sites in the meningococcal chromosome involved in biosynthesis of the oligosaccharide chain of LOS and to create genetically defined LOS mutants of N. meningitidis and Neisseria gonorrhoeae. Images PMID:7516313

Stephens, D S; McAllister, C F; Zhou, D; Lee, F K; Apicella, M A

1994-01-01

158

Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides.  

PubMed Central

The transposon Tn951 (lac) was introduced into the photosynthetic bacterium Rhodopseudomonas sphaeroides 2.4.1, which is normally Lac-, via the P-group plasmid RP1. beta-Galactosidase was produced constitutively in both chemotrophically and phototrophically grown cells, and the levels were found to be the same but low. Mutants were isolated, however, that were able to grow on lactose minimal medium and which expressed different levels of beta-galactosidase when grown chemotrophically or phototrophically. The beta-galactosidase levels found in all R. sphaeroides strains were much less than those found in Escherichia coli. PMID:6290458

Nano, F E; Kaplan, S

1982-01-01

159

5. Photocopy of May 21, 1936 photograph showing the interior ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Photocopy of May 21, 1936 photograph showing the interior of the Big House (main portion, living-bedroom). The woman is Hettie Walker. The fireplace is on the house's front side. The original photograph was taken by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8086. - Walker Family Farm, Big House, Gatlinburg, Sevier County, TN

160

Ca(2+)-regulatory function of the inhibitory peptide region of cardiac troponin I is aided by the C-terminus of cardiac troponin T: Effects of familial hypertrophic cardiomyopathy mutations cTnI R145G and cTnT R278C, alone and in combination, on filament sliding.  

PubMed

Investigations of cardiomyopathy mutations in Ca(2+) regulatory proteins troponin and tropomyosin provide crucial information about cardiac disease mechanisms, and also provide insights into functional domains in the affected polypeptides. Hypertrophic cardiomyopathy-associated mutations TnI R145G, located within the inhibitory peptide (Ip) of human cardiac troponin I (hcTnI), and TnT R278C, located immediately C-terminal to the IT arm in human cardiac troponin T (hcTnT), share some remarkable features: structurally, biochemically, and pathologically. Using bioinformatics, we find compelling evidence that TnI and TnT, and more specifically the affected regions of hcTnI and hcTnT, may be related not just structurally but also evolutionarily. To test for functional interactions of these mutations on Ca(2+)-regulation, we generated and characterized Tn complexes containing either mutation alone, or both mutations simultaneously. The most important results from in vitro motility assays (varying [Ca(2+)], temperature or HMM density) show that the TnT mutant "rescued" some deleterious effects of the TnI mutant at high Ca(2+), but exacerbated the loss of function, i.e., switching off the actomyosin interaction, at low Ca(2+). Taken together, our experimental results suggest that the C-terminus of cTnT aids Ca(2+)-regulatory function of cTnI Ip within the troponin complex. PMID:24418317

Brunet, Nicolas M; Chase, P Bryant; Mihajlovi?, Goran; Schoffstall, Brenda

2014-06-15

161

The Sugar Creek zinc deposit, Jackson Co. TN -- Exploration history, geology and mineralization  

SciTech Connect

During the 60's and 70's zinc exploration of central TN and KY was active. The Sugar Creek Project was one of several investigated by Exxon. The discovery hole, Cu 15, was drilled in early 1973. The Sugar Creek Zinc Deposit was acquired by Independence Mining Co. in 1986 and I.M.C. has subsequently completed additional drilling, both stepout and confirmation holes. A total of 137 holes for 300,833 ft have been drilled. The Sugar Creek deposit is a typical Tennessee zinc deposit (Mississippi Valley Type) which occurs in solution collapse breccias in the Lower Ordovician, Knox Dolomite. The Knox consists of fine grained dolomite with interlayered limestones and crystalline dolomite. Only scattered residual limestone is found in the Sugar Creek area. Collapse breccias have formed which control zinc deposition and are similar to other TN Zn. deposits. At Sugar Creek the types of breccias include: a vertically exaggerated glory hole breakthrough breccia which extends to within 137 ft. of the Knox unconformity, has 500 ft. of zinc mineralization with 8 significant zinc intervals; holes with stacked zinc intervals interpreted to be sides of breakthrough breccia; and single zinc intervals in laterally positioned bedded mineral zones. A total of 99 holes were drilled in the more intense mineralized areas. The ratio of ore to non ore holes is nearly 1 to 1. The mineralization is typical M.V.T. with predominantly sphalerite and only minor occurrences of galena, fluorite, pyrite, etc.

Reinbold, G.; Moran, A.V.; Stevens, D.L. (Independence Mining Co. Inc., Reno, NV (United States))

1993-03-01

162

Regulatory roles of spnT, a novel gene located within transposon TnTIR.  

PubMed

The transposon TnTIR contains spnIR quorum-sensing system regulating sliding motility and the production of nuclease, biosurfactant, and prodigiosin in Serratia marcescens. Within TnTIR, a gene named spnT is upstream of and co-transcribed with spnI. SpnT is a cytoplasmic protein and its level peaks during early stationary phase. spnT over-expression resulted in inhibition of sliding motility and synthesis of prodigiosin, and biosurfactant similar to spnR. spnT but not spnR over-expression induced cell elongation and aberrant DNA replication in S. marcescens and Escherichia coli strains. In comparison with wild-type E. coli strain, over-expression of spnT in an E. coli priA and dnaC double-mutant strain did not lead to the aberrant cell morphology phenotypes, suggesting SpnT may act through the recombination-dependent DNA replication system. As spnT over-expression inhibited swarming but not swimming motility, SpnT may indirectly function as a negative regulator of surface-dependent migration and secondary metabolite production. PMID:16899219

Wei, Jun-Rong; Soo, Po-Chi; Horng, Yu-Tze; Hsieh, Shang-Chen; Tsai, Yu-Huan; Swift, Simon; Withers, Helen; Williams, Paul; Lai, Hsin-Chih

2006-09-29

163

Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model  

PubMed Central

Changes in the composition of glycans added to glycoproteins and glycolipids are characteristic of the change to malignancy. Sialyl-Tn (STn) is expressed by 2530% of breast carcinomas but its expression on normal tissue is highly restricted. Sialyl-Tn is an O-linked disaccharide that can be carried on various glycoproteins. One such glycoprotein MUC1 is expressed by the vast majority of breast carcinomas. Both STn and MUC1 have been considered as targets for immunotherapy of breast cancer patients. Here we used different immunogens to target STn in an MUC1 transgenic mouse model of tumour challenge. We show that synthetic STn coupled to keyhole limpet haemocyanin (Theratope), induced antibodies to STn that recognised the glycan carried on a number of glycoproteins and in these mice a significant delay in tumour growth was observed. The protection was dependant on STn being expressed by the tumour and was antibody mediated. Affinity chromatography of the STn-expressing tumour cell line, followed by mass spectrometry, identified osteopontin as a novel STn-carrying glycoprotein which was highly expressed by the tumours. These results suggest that if antibodies can be induced to a number of targets expressed by the tumour cells, a humoral response can be effective in controlling tumour growth. PMID:19436292

Julien, S; Picco, G; Sewell, R; Vercoutter-Edouart, A-S; Tarp, M; Miles, D; Clausen, H; Taylor-Papadimitriou, J; Burchell, J M

2009-01-01

164

Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha  

SciTech Connect

Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the inability to grow with reduced sulfur compounds (Sox/sup -/). Four classes of Sox/sup -/ mutants were obtained. Three were of different pleiotropic phenotypes: (i) unable to grow with formate, nitrate, and xanthine; (this class strongly suggested the involvement of a molybdenum cofactor in inorganic sulfur-oxidizing ability); (ii) no growth with hydrogen; (iii) slight growth with hydrogen and formate. Two plasmids, pHG41 (about 450 kilobase pairs) and pHG42 (110 kilobases), were identified in lysates of T. pantotropha. In one Sox/sup -/ mutant pHG41 could not be detected. Revertant analysis suggested that pHG41 and pHG42 were not involved in the Sox character.

Chandra, T.S.; Friedrich, C.G.

1986-05-01

165

Isolation of a Tn501 insertion mutant lacking porin protein P of Pseudomonas aeruginosa.  

PubMed

In order to demonstrate a role for anion-specific protein P channels in phosphate transport in Pseudomonas aeruginosa PAO, we wished to isolate a transposon insertion mutant deficient in protein P. A number of transposon delivery systems were tested which yielded, for the most part, whole plasmid inserts. Plasmid pMT1000 (Tsuda et al. 1984), a temperature-sensitive R68 plasmid carrying the transposon Tn501, was successfully employed in the isolation of a Tn501 insertion mutant lacking protein P under normally inducing conditions. To identify the mutant deficient in protein P, a protein P-specific polyclonal antiserum was used. This mutant, strain H576, was deficient in high-affinity phosphate transport exhibiting a Km for uptake (3.60 +/- 0.64 microM) almost ten times greater than that of the wild type strain (Km = 0.39 microM). There was, however, no change in the Vmax for high-affinity phosphate transport as a result of the loss of protein P in this mutant. The protein P-deficiency of the mutant correlated with a growth defect in a phosphate-limited medium, resulting in an 18%-35% decrease in growth when compared with the wild type. PMID:2423845

Poole, K; Hancock, R E

1986-03-01

166

32 CFR 1602.10 - County.  

Code of Federal Regulations, 2011 CFR

...Defense 6 2011-07-01 2011-07-01 false County. 1602.10 Section 1602.10 National Defense...SELECTIVE SERVICE SYSTEM DEFINITIONS 1602.10 County. The word county includes, where applicable, counties,...

2011-07-01

167

32 CFR 1602.10 - County.  

Code of Federal Regulations, 2012 CFR

...Defense 6 2012-07-01 2012-07-01 false County. 1602.10 Section 1602.10 National Defense...SELECTIVE SERVICE SYSTEM DEFINITIONS 1602.10 County. The word county includes, where applicable, counties,...

2012-07-01

168

32 CFR 1602.10 - County.  

Code of Federal Regulations, 2010 CFR

...Defense 6 2010-07-01 2010-07-01 false County. 1602.10 Section 1602.10 National Defense...SELECTIVE SERVICE SYSTEM DEFINITIONS 1602.10 County. The word county includes, where applicable, counties,...

2010-07-01

169

Integrative gene cloning and expression system for Streptomyces sp. US 24 and Streptomyces sp. TN 58 bioactive molecule producing strains.  

PubMed

Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch. PMID:19547659

Sioud, Samiha; Aigle, Bertrand; Karray-Rebai, Ines; Smaoui, Slim; Bejar, Samir; Mellouli, Lotfi

2009-01-01

170

The Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD  

PubMed Central

The excision of transposon Tn7 from a donor site and its insertion into its preferred target site, attachment site attTn7, is mediated by four Tn7-encoded transposition proteins: TnsA, TnsB, TnsC, and TnsD. Transposition requires the assembly of a nucleoprotein complex containing all four Tns proteins and the DNA substrates, the donor site containing Tn7, and the preferred target site attTn7. TnsA and TnsB together form the heteromeric Tn7 transposase, and TnsD is a target-selecting protein that binds specifically to attTn7. TnsC is the key regulator of transposition, interacting with both the TnsAB transposase and TnsD-attTn7. We show here that TnsC interacts directly with TnsB, and identify the specific region of TnsC involved in the TnsBTnsC interaction during transposition. We also show that a TnsC mutant defective in interaction with TnsB is defective for Tn7 transposition both in vitro and in vivo. Tn7 displays cis-acting target immunity, which blocks Tn7 insertion into a target DNA that already contains Tn7. We provide evidence that the direct TnsBTnsC interaction that we have identified also mediates cis-acting Tn7 target immunity. We also show that TnsC interacts directly with the target selector protein TnsD. PMID:24982178

Choi, Ki Young; Spencer, Jeanelle M.; Craig, Nancy L.

2014-01-01

171

Budgeting in Texas Counties, 1931-1940.  

E-print Network

the ~arions associations of county officers, particularly the county judges and commissioners, the county auditors, and the county dmlcs. CONTENTS Introduction 5 Purpose of Study 6 Procedure 6 Legal Provisions 9 The Budget Form 11 Number of Budgets... the ~arions associations of county officers, particularly the county judges and commissioners, the county auditors, and the county dmlcs. CONTENTS Introduction 5 Purpose of Study 6 Procedure 6 Legal Provisions 9 The Budget Form 11 Number of Budgets...

Bradshaw, H. C.

1941-01-01

172

Hillsborough County Cooperative Extension Service  

E-print Network

Hillsborough County Cooperative Extension Service 5339 County Rd 579 Seffner, Fl., 33584-3334 813 Irrigation Systems Dave Palmer - Extension Agent I - Hillsborough county Extension Service As of this writing of timers, control clocks, and water meters. Similarly, the homeowner could leave the house or go to sleep

Jawitz, James W.

173

STATE/COUNTY BORDERS  

EPA Science Inventory

This data is available for the entire region. The level of detail is primarily suited for region/state/county/basin sized maps. The borders are not accurate enough for small areas like sub-basins and site areas. We use this coverage for general backgrounds and borders only. T...

174

Westchester County Employers Survey.  

ERIC Educational Resources Information Center

The Westchester County Employers Survey was done in May 2003 in order learn more about employee training needs, how they accomplished these needs, and how it would be possible for the Westchester Community College to fulfill these needs. Out of the 639 surveys sent, 145 were returned in a satisfactory format, which produces a 22.6% return rate.

Lee, Marcia M.

175

San Diego County Reservation  

E-print Network

San Diego County Brenda Pisgah Iron Mountain Riverside East Imperial East Morongo Reservation) Reservation 60 95 95 95 115 San Diego Banning Yucca Valley Twentynine Palms Desert Hot Springs Palm Springs and Implement Agency-Specific Programs for Solar Energy Development San Francisco San Diego Los Angeles

Laughlin, Robert B.

176

Henry County School Questionnaire.  

ERIC Educational Resources Information Center

This 14-item questionnaire was designed to measure parent opinion regarding the effect of integration on third grade pupils in Henry County Schools. The questionnaire is not standardized, and field testing has been on a small scale. (See also TM 000 940 for a description of the study, and 942, 943 for the desegregation and school integration

Goolsby, Thomas M., Jr.; Frary, Robert B.

177

A double diastereoselective Michael-type addition as an entry to conformationally restricted tn antigen mimics.  

PubMed

A totally stereocontrolled C-Michael addition of serine-equivalent C-nucleophiles to tri-O-benzyl-2-nitro-d-galactal was used as the key step to synthesize several pyrano[3,2-b]pyrrole structures. These scaffolds could be regarded as conformationally restricted Tn antigen mimics, as we have demonstrated by biological assays. The pyranose rings retain their (4)C1 chair conformation, as shown by molecular modeling and NMR spectroscopy. The expected bioactivity was established by a competition-tailored enzyme-linked lectin assay using both soybean and Vicia villosa agglutinins as model lectins. The facile described synthetic route and the strategic combination of computational and experimental techniques to reveal conformational features and bioactivity demonstrate the prepared glycomimics to be promising candidates for further exploitation of this scaffold to give glycans for lectin blocking and vaccination. PMID:24083620

Aydillo, Carlos; Navo, Claudio D; Busto, Jess H; Corzana, Francisco; Zurbano, Mara M; Avenoza, Alberto; Peregrina, Jess M

2013-11-01

178

Cardiac troponin I but not cardiac troponin T adheres to polysulfone dialyser membranes in an in vitro haemodialysis model: explanation for lower serum cTnI concentrations following dialysis  

PubMed Central

Background Elevated serum cardiac troponin T (cTnT) and I (cTnI) can occur in patients with chronic kidney disease. Differences in cTn concentrations between cTnT and cTnI have been reported but the mechanism of such discrepancy has not been investigated. This study investigates the clearance of cTn with the aid of an in vitro model of haemodialysis (HD). Methods Serum was obtained before and after a single session of dialysis from 53 patients receiving HD and assayed for cTnT and cTnI. An in vitro model of the dialysis process was used to investigate the mechanism of clearance of cTn during HD. Results Serum cTnI was significantly lower (p=0.043) following a session of HD whereas cTnT concentrations were similar to those obtained before HD. Using an in vitro model of dialysis, it was demonstrated that cTnI is not dialysed from the vascular compartment but adheres to the dialyser membrane. Conclusions The adherence of cTnI to the dialyser membrane is responsible for the observed decrease in serum cTnI following a session of dialysis. The adherence of cTnT or T-I-C complex to the dialyser membrane could not be demonstrated and supports the observation that pre-HD and post-HD serum concentrations of cTnT are similar. PMID:25332816

Gaze, David C; Collinson, Paul O

2014-01-01

179

Tn5-Induced and Spontaneous Switching of Sinorhizobium meliloti to Faster-Swarming Behavior  

PubMed Central

Tn5 mutants of Sinorhizobium meliloti RMB7201 which swarmed 1.5 to 2.5 times faster than the parental strain in semisolid agar, moist sand, and viscous liquid were identified. These faster-swarming (FS) mutants outgrew the wild type 30- to 40-fold within 2 days in mixed swarm colonies. The FS mutants survived and grew as well as or better than the wild type under all of the circumstances tested, except in a soil matrix subjected to air drying. Exopolysaccharide (EPS) synthesis was reduced in each of the FS mutants when they were grown on defined succinate-nitrate medium, but the extent of reduction was different for each. It appears that FS behavior likely results from a modest, general derepression of motility involving an increased proportion of motile and flagellated cells and an increased average number of flagella per cell and increased average flagellar length. Spontaneous FS variants of RMB7201 were obtained at a frequency of about 1 per 10,000 to 20,000 cells by either enrichment from the periphery of swarm colonies or screening of colonies for reduced EPS synthesis on succinate-nitrate plates. The spontaneous FS variants and Tn5 FS mutants were symbiotically effective and competitive in alfalfa nodulation. Reversion of FS variants to wild-type behavior was sporadic, indicating that reversion is affected by unidentified environmental factors. Based on phenotypic and molecular differences between individual FS variants and mutants, it appears that there may be multiple genetic configurations that result in FS behavior in RMB7201. The facile isolation of spontaneous FS variants of Escherichia coli and Pseudomonas aeruginosa indicates that switching to FS behavior may be fairly common among bacterial species. The substantial growth advantage of FS mutants and variants wherever nutrient gradients exist suggests that switching to FS forms may be an important behavioral adaptation in natural environments. PMID:10049888

Wei, Xueming; Bauer, Wolfgang D.

1999-01-01

180

TN-68 Spent Fuel Transport Cask Analytical Evaluation for Drop Events  

SciTech Connect

The U.S. Nuclear Regulatory Commission (NRC) is responsible for licensing commercial spent nuclear fuel transported in casks certified by NRC under the Code of Federal Regulations (10 CFR), Title 10, Part 71 [1]. Both the International Atomic Energy Agency regulations for transporting radioactive materials [2, paragraph 727], and 10 CFR 71.73 require casks to be evaluated for hypothetical accident conditions, which includes a 9-meter (m) (30-ft) drop-impact event onto a flat, essentially unyielding, horizontal surface, in the most damaging orientation. This paper examines the behavior of one of the NRC certified transportation casks, the TN-68 [3], for drop-impact events. The specific area examined is the behavior of the bolted connections in the cask body and the closure lid, which are significantly loaded during the hypothetical drop-impact event. Analytical work to evaluate the NRC-certified TN-68 spent fuel transport cask [3] for a 9-m (30-ft) drop-impact event on a flat, unyielding, horizontal surface, was performed using the ANSYS [4] and LS DYNA [5] finite-element analysis codes. The models were sufficiently detailed, in the areas of bolt closure interfaces and containment boundaries, to evaluate the structural integrity of the bolted connections under 9-m (30-ft) free-drop hypothetical accident conditions, as specified in 10 CFR 71.73. Evaluation of the cask for puncture, caused by a free drop through a distance of 1-m (40-in.) onto a mild steel bar mounted on a flat, essentially unyielding, horizontal surface, required by 10 CFR 71.73, was not included in the current work, and will have to be addressed in the future. Based on the analyses performed to date, it is concluded that, even though brief separation of the flange and the lid surfaces may occur under some conditions, the seals would close at the end of the drop events, because the materials remain elastic during the duration of the event.

Shah, M. J.; Klymyshyn, Nicholas A.; Adkins, Harold E.; Koeppel, Brian J.

2007-03-30

181

Evolutionary relationship between Tn 21 -like elements and pBP201, a plasmid from Klebsiella pneumoniae mediating resistance to gentamicin and eight other drugs  

Microsoft Academic Search

We have characterized pBP201 one of the plasmids from a collection of 46 strains producing adenylyltransferase ANT (2?) (Schmidt 1984). It confers resistance to sulphonamides and produces aminoglycoside adenylyltransferases AAD (3?) and ANT (2?) and -lactamase TEM-1. Plasmid pBP201 has a size of 24.8 kilobases (kb) and contains TnA and a Tn21-related element, Tn4000?, with deletions in mer and the

Friedrich Schmidt; Ingrid Klopfer-Kaul

1984-01-01

182

Interaction of the chromosomal Tn557 with two thermosensitive derivatives, pS1 and p ?D, of the plasmid pI9789 in Staphylococcus aureus  

Microsoft Academic Search

The plasmid pI9789::Tn552 carries genes conferring resistance to penicillins and to cadmium, mercury and arsenate ions. The presence of Tn551 at one location in the chromosome of Staphylococcus aureus enhances the frequency of suppression of thermosensitivity of replication of the plasmids pSl and p?D which are derivatives of pI9789::Tn552. Bacteriophage propagated on the bacteria in which thermosensitivity of replication had

Muhammad Sohail; Mike Oldridge; Keith G. H Dyke

1995-01-01

183

75 FR 70076 - Tennessee Southern Railroad Company, Patriot Rail, LLC, Patriot Rail Holdings LLC, and Patriot...  

Federal Register 2010, 2011, 2012, 2013

...milepost 8.5, including auxiliary and spur tracks, in Cowlitz County, Wash.; Docket...including auxiliary, temporary storage, and spur tracks, in Howard and Sevier Counties...0, including auxiliary, storage, and spur tracks, in McCurtain County,...

2010-11-16

184

County Business Patterns 1995  

NSDL National Science Digital Library

The US Census Bureau has made available the state publications for the 1995 version of its annual County Business Patterns 1995 series (Adobe Acrobat [.pdf] format only). Data available include employment, payroll, and number of establishments by Standard Industrial Code category. Establishments are further broken down by nine employment size classes ranging from 1-4 to 1,000 or more. The US volume is forthcoming. Complete 1993 and 1994 (also .pdf format) series are available at the site.

1997-01-01

185

Purchasing in Texas Counties.  

E-print Network

contracts are made for the purchase of record books, letterheads, miscellaneous stationery items, drugs, laundry work, electric light bulbs, and milk. Quarterly contracts are made for the purchase of bread for the county farm and jail, and monthly... of lumber would be $70 in the case of treated lumber and $220 in the case of untreated lumber. These figures apply to a period of time measured by the life of a treated lumber struc- ture. 8 BULLETIN NO. 653, TEXAS. AGRICULTURAL EXPERIMENT STATION...

Hervey, E. J.; Bradshaw, H. C.

1944-01-01

186

Analysis of twisted nematic liquid crystal FabryPerot interferometer (TN-FPI) filter based on the coupled mode theory  

Microsoft Academic Search

The tuning characteristics of a twisted nematic (TN) liquid crystal (LC) FabryPerot interferometer (FPI) wavelength filter, which is a basic component of wavelength division multiplexed transmission systems, is analyzed using coupled mode theory. First, a simplified modeling of the refractive index change in the LC layer is presented. Next, various characteristics of the eigen polarization modes of the LC medium

Y. Ohtera; H. Yoda; S. Kawakami

2000-01-01

187

Diophantine equations with products of consecutive terms in Lucas sequences F. Luca and T.N. Shorey  

E-print Network

Diophantine equations with products of consecutive terms in Lucas sequences F. Luca and T.N. Shorey the product of consecutive terms in arithmetic progressions is replaced by the product of terms in Lucas for the Lucas sequences of first and second kind, respectively, of general terms u n = # n - # n # - # for all n

Shorey, T.N.

188

ION GPS '99, 14-17 September 1999, Nashville, TN Testing a GPS-based System for the Support of  

E-print Network

ION GPS '99, 14-17 September 1999, Nashville, TN Testing a GPS-based System for the Support of Ship as research professional on the GPS OTF project within the GEOIDE Network of Centres of Excellence of Geomatic Sciences at Laval University, where he conducts research and teaching in precise GPS static

Santerre, Rock

189

Identification of a catabolic transposon, Tn4371, carrying biphenyl and 4-chlorobiphenyl degradation genes in Alcaligenes eutrophus A5.  

PubMed Central

Alcaligenes eutrophus A5 catabolizes biphenyl to CO2 via benzoate and 4-chlorobiphenyl to 4-chlorobenzoate. In curing and conjugation experiments, the A5 endogenous 51-kb IncP1 plasmid pSS50 was found to be dispensable for biphenyl and 4-chlorobiphenyl catabolism. Transfer of the biphenyl- and 4-chlorobiphenyl-degrading phenotype by means of pSS50 was observed at a frequency of 10(-5) per transferred plasmid in matings of A5 with other A. eutrophus strains. Transconjugants harbor enlarged pSS50 derivatives which contain additional genetic information governing the oxidation of biphenyl and 4-chlorobiphenyl to benzoate and 4-chlorobenzoate and originating from the chromosome of strain A5. The following observations indicate that the catabolic genes reside on a 59-kb large transposon (Tn4371) for which a restriction map is presented. (i) Tn4371 transposes between different replicons and at different locations of the same replicon. (ii) Transposition was observed in a Rec- strain of A. eutrophus. (iii) Tn4371 transposes as a single, contiguous piece of DNA. Although an RP4::Tn4371 plasmid was stably maintained in different hosts, the plasmid conferred growth on biphenyl only when present in strains of A. eutrophus and in an Acinetobacter sp. strain. Images PMID:8383664

Springael, D; Kreps, S; Mergeay, M

1993-01-01

190

313 Conference Center, Knoxville, TN 37996-4137 865-974-0150 Kids U Parental Consent Form  

E-print Network

in activities related to Kids U. Furthermore, we (I) are the parent(s) or legal guardian(s) of this participant recreational activities have inherent risks. We (l) also authorize our (my) child to have fun and hopefully313 Conference Center, Knoxville, TN 37996-4137 · 865-974-0150 Kids U Parental Consent Form Please

Wang, Xiaorui "Ray"

191

Modular Evolution of TnGBSs, a New Family of Integrative and Conjugative Elements Associating Insertion Sequence Transposition, Plasmid Replication, and Conjugation for Their Spreading  

PubMed Central

Integrative and conjugative elements (ICEs) have a major impact on gene flow and genome dynamics in bacteria. The ICEs TnGBS1 and TnGBS2, first identified in Streptococcus agalactiae, use a DDE transposase, unlike most characterized ICEs, which depend on a phage-like integrase for their mobility. Here we identified 56 additional TnGBS-related ICEs by systematic genome analysis. Interestingly, all except one are inserted in streptococcal genomes. Sequence comparison of the proteins conserved among these ICEs defined two subtypes related to TnGBS1 or TnGBS2. We showed that both types encode different conjugation modules: a type IV secretion system, a VirD4 coupling protein, and a relaxase and its cognate oriT site, shared with distinct lineages of conjugative elements of Firmicutes. Phylogenetic analysis suggested that TnGBSs evolved from two conjugative elements of different origins by the successive recruitment of a transposition module derived from insertion sequences (ISs). Furthermore, TnGBSs share replication modules with different plasmids. Mutational analyses and conjugation experiments showed that TnGBS1 and TnGBS2 combine replication and transposition upstream promoters for their transfer and stabilization. Despite an evolutionarily successful horizontal dissemination within the genus Streptococcus, these ICEs have a restricted host range. However, we reveal that for TnGBS1 and TnGBS2, this host restriction is not due to a transfer incompatibility linked to the conjugation machineries but most likely to their ability for transient maintenance through replication after their transfer. PMID:23435978

Gurillot, Romain; Da Cunha, Violette; Sauvage, Elisabeth; Bouchier, Christiane

2013-01-01

192

Impaired relaxation is the main manifestation in transgenic mice expressing a restrictive cardiomyopathy mutation, R193H, in cardiac TnI  

PubMed Central

Transgenic mice were generated to express a restrictive cardiomyopathy (RCM) human cardiac troponin I (cTnI) R192H mutation in the heart (cTnI193His mice). The objective of this study was to assess cardiac function during the development of diastolic dysfunction and to gain insight into the pathophysiological impact of the RCM cTnI mutation. Cardiac function and pathophysiological changes were monitored in cTnI193His mice and wild-type littermates for a period of 12 mo. It progressed gradually from abnormal relaxation to diastolic dysfunction characterized with high-resolution echocardiography by a reversed E-to-A ratio, increased deceleration time, and prolonged isovolumetric relaxation time. At the age of 12 mo, cardiac output in cTnI193His mice was significantly declined, and some transgenic mice showed congestive heart failure. The negative impact of cTnI193His on ventricular contraction and relaxation was further demonstrated in isolated mouse working heart preparations. The main morphological change in cTnI193His myocytes was shortened cell length. Dobutamine stimulation increased heart rate in cTnI193His mice but did not improve CO. The cTnI193His mice had a phenotype similar to that in human RCM patients carrying the cTnI mutation characterized morphologically by enlarged atria and restricted ventricles and functionally by diastolic dysfunction and diastolic heart failure. The results demonstrate a critical role of the COOH-terminal domain of cTnI in the diastolic function of cardiac muscle. PMID:18408133

Du, Jianfeng; Liu, Jing; Feng, Han-Zhong; Hossain, M. M.; Gobara, Nariman; Zhang, Chi; Li, Yuejin; Jean-Charles, Pierre-Yves; Jin, Jian-Ping; Huang, Xu-Pei

2009-01-01

193

Robust rat pulmonary radioprotection by a lipophilic Mn N-alkylpyridylporphyrin, MnTnHex-2-PyP5+?  

PubMed Central

With the goal to enhance the distribution of cationic Mn porphyrins within mitochondria, the lipophilic Mn(III)meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin, MnTnHex-2-PyP5+ has been synthesized and tested in several different model of diseases, where it shows remarkable efficacy at as low as 50g/kg single or multiple doses. Yet, in a rat lung radioprotection study, at higher 0.61mg/kg doses, due to its high accumulation and micellar character, it became toxic. To avoid the toxicity, herein the pulmonary radioprotection of MnTnHex-2-PyP5+ was assessed at 50g/kg. Fischer rats were irradiated to their right hemithorax (28Gy) and treated with 0.05mg/kg/day of MnTnHex-2-PyP5+ for 2 weeks by subcutaneously-implanted osmotic pumps, starting at 2h post-radiation. The body weights and breathing frequencies were followed for 10 weeks post-radiation, when the histopathology and immunohistochemistry were assessed. Impact of MnTnHex-2-PyP5+ on macrophage recruitment (ED-1), DNA oxidative damage (8-OHdG), TGF-?1, VEGF(A) and HIF-1? were measured. MnTnHex-2-PyP5+ significantly decreased radiation-induced lung histopathological (H&E staining) and functional damage (breathing frequencies), suppressed oxidative stress directly (8-OHdG), or indirectly, affecting TGF-?1, VEGF (A) and HIF-1? pathways. The magnitude of the therapeutic effects is similar to the effects demonstrated under same experimental conditions with 120-fold higher dose of ~5000-fold less lipophilic Mn(III)meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin, MnTE-2-PyP5+. PMID:24624330

Gauter-Fleckenstein, Benjamin; Reboucas, Julio S.; Fleckenstein, Katharina; Tovmasyan, Artak; Owzar, Kouros; Jiang, Chen; Batinic-Haberle, Ines; Vujaskovic, Zeljko

2014-01-01

194

77 FR 73005 - Revisions to the California State Implementation Plan, Imperial County, Placer County, and...  

Federal Register 2010, 2011, 2012, 2013

...Placer County, and Ventura County Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD)...

2012-12-07

195

77 FR 72968 - Revisions to the California State Implementation Plan, for Imperial County, Placer County and...  

Federal Register 2010, 2011, 2012, 2013

...Placer County and Ventura County Air Pollution Control Districts AGENCY: Environmental...revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD)...

2012-12-07

196

75 FR 49016 - County of Greenville, S.C.-Acquisition Exemption-Greenville County Economic Development Corporation  

Federal Register 2010, 2011, 2012, 2013

...Exemption--Greenville County Economic Development Corporation The County of...acquire from Greenville County Economic Development Corporation (GCEDC) approximately...See Greenville County Economic Development...

2010-08-12

197

Sialyl-tn in cancer: (how) did we miss the target?  

PubMed

Sialyl-Tn antigen (STn) is a short O-glycan containing a sialic acid residue a2,6-linked to GalNAca-O-Ser/Thr. The biosynthesis of STn is mediated by a specific sialyltransferase termed ST6GalNAc I, which competes with O-glycans elongating glycosyltransferases and prevents cancer cells from exhibiting longer O-glycans. While weakly expressed by fetal and normal adult tissues, STn is expressed by more than 80% of human carcinomas and in all cases, STn detection is associated with adverse outcome and decreased overall survival for the patients. Because of its pan-carcinoma expression associated with an adverse outcome, an anti-cancer vaccine, named Theratope, has been designed towards the STn epitope. In spite of the great enthusiasm around this immunotherapy, Theratope failed on Phase III clinical trial. However, in lieu of missing this target, one should consider to revise the Theratope design and the actual facts. In this review, we highlight the many lessons that can be learned from this failure from the immunological standpoint, as well as from the drug design and formulation and patient selection. Moreover, an irrefutable knowledge is arising from novel immunotherapies targeting other carbohydrate antigens and STn carrier proteins, such as MUC1, that will warrantee the future development of more successful anti-STn immunotherapy strategies. PMID:24970145

Julien, Sylvain; Videira, Paula A; Delannoy, Philippe

2012-01-01

198

Sialyl-Tn in Cancer: (How) Did We Miss the Target?  

PubMed Central

Sialyl-Tn antigen (STn) is a short O-glycan containing a sialic acid residue ?2,6-linked to GalNAc?-O-Ser/Thr. The biosynthesis of STn is mediated by a specific sialyltransferase termed ST6GalNAc I, which competes with O-glycans elongating glycosyltransferases and prevents cancer cells from exhibiting longer O-glycans. While weakly expressed by fetal and normal adult tissues, STn is expressed by more than 80% of human carcinomas and in all cases, STn detection is associated with adverse outcome and decreased overall survival for the patients. Because of its pan-carcinoma expression associated with an adverse outcome, an anti-cancer vaccine, named Theratope, has been designed towards the STn epitope. In spite of the great enthusiasm around this immunotherapy, Theratope failed on Phase III clinical trial. However, in lieu of missing this target, one should consider to revise the Theratope design and the actual facts. In this review, we highlight the many lessons that can be learned from this failure from the immunological standpoint, as well as from the drug design and formulation and patient selection. Moreover, an irrefutable knowledge is arising from novel immunotherapies targeting other carbohydrate antigens and STn carrier proteins, such as MUC1, that will warrantee the future development of more successful anti-STn immunotherapy strategies. PMID:24970145

Julien, Sylvain; Videira, Paula A.; Delannoy, Philippe

2012-01-01

199

Tn5-Mob transposon mediated transfer of salt tolerance and symbiotic characteristics between Rhizobia genera.  

PubMed

Rhizobium meliloti 042B is a fast-growing, salt-tolerant and high efficiency nitrogen-fixing symbiont with alfalfa. Bradyrhizobium japonicum USDA110 grows slowly, and cannot grow in YMA medium containing 0.1M NaCl, but nodulates and fixed nitrogen efficiently with soybean. Eighty-six transconjugants, called SR, were obtained by inserting Tn5-Mob randomly into genomes of 042B using pSUP5011 and helper plasmid RP4. Selecting 4 SR strains randomly and introducing DNA fragment of SR into USDA110 with helper plasmid R68.45 by triparental mating, 106 transconjugants, called BSR, were constructed. Most of BSR strains had the fast-growing phenotype and could tolerate 0.3-0.5M NaCl generally. Some of them produced melanine. When soybean and alfalfa were inoculated with these transconjugants BSR, 47 out of 90 BSR were found to nodulate in both of these plants, but no nitrogenase activity was observed with alfalfa; 26 strains could only nodulate and fix nitrogen in soybean; 13 strains could nodulate in alfalfa but did not fix nitrogen; 4 strains failed to nodulate in either soybean or alfalfa. Among them, 4 transconjugants which tolerated and fixed nitrogen efficiently in soybean were constructed. PMID:8049344

Yang, S; Wu, Z; Gao, W; Li, J

1993-01-01

200

Characterization of the tet(M) determinant of Tn916: evidence for regulation by transcription attenuation.  

PubMed Central

The nucleotide sequence of the tetracycline resistance determinant tet(M), located on conjugative transposon Tn916 of Enterococcus faecalis, was determined and found to encode a 72,486-dalton protein exhibiting a high degree of homology with other tet(M) determinants. A short open reading frame corresponding to a 28-amino-acid peptide and containing a number of inverted repeat sequences was noted immediately upstream of tet(M), suggesting that regulation might occur by a mechanism involving transcriptional attenuation. Transcription analyses found this to indeed be the case, showing that the expression of tet(M) resulted from an extension of a small transcript representing the upstream leader region into the resistance determinant. Exposure of cells to tetracycline resulted in a significant increase in the amount of tet(M) transcription; this increase could be explained on the basis of increased transcriptional read-through from the upstream transcript. A model suggesting how transcriptional attenuation might operate in this system is presented. Images PMID:1323953

Su, Y A; He, P; Clewell, D B

1992-01-01

201

EEO Employment Data Allegheny County  

E-print Network

EEO Employment Data for Allegheny County and the Pittsburgh region February 2008 University Center................................................................................................................. 2 Employment Summary by EEO Occupation Group............................................................................... 3 Employment by Detail Census Occupation Category

Sibille, Etienne

202

Digital Atlas of Texas Counties  

NSDL National Science Digital Library

Created by the Center for Geosptial Technology at Texas Tech University, this digital atlas is a fantastic find. It brings together information about all of Texas's counties, including satellite relief maps and data sets about the roads, rivers, lakes, and other features in each area. Visitors can use the interactive map to click on a county of interest, or use the drop down menu to select a region. Once users select a county, they can download information about it for future use. The site also contains a number of Featured Links to resources from the U.S. Census Bureau, the Texas Association of Counties, and several tourism agencies.

203

A rapid and simple method for the isolation of mutant variants regulating tissue-specific expression of the TnI gene through drug selection  

SciTech Connect

TnINEO fusion gene was constructed by fusing 3.4-kbp of quail TnI genomic DNA sequences spanning the promoter to exon 5 and a neo gene in frame. A myoblast cell line was established after transfection of pTnINEO. Since this cell line was passaged several times, a high frequency of neomycin (G418) sensitivity conversion was detected. Two drug-resistant variants were analyzed through genomic Southern blot and S1 nuclease protection assay. One variant has a mutation(s) in the regulatory element that activated the dormant TnI promoter-enhancer in myoblast, and the other has shown the geonomic rearrangement. This result presented the possibility of isolating factor(s) that activate the muscle-specific TnI promoter simply by screening drug-resistant cells having appropriate mutations. 12 refs., 4 fig.

Lee, Youngwon; Kim, Myoung Hee [Korea Research Institute of Bioscience and Biotechnology, Taejon (Korea, Republic of); Emerson, C.P. Jr. [Fox Chase Cancer Center, Philadelphia, PA (United States)

1995-12-01

204

Taiwan Nantou County earthquake 0327 Taiwan Nantou County earthquake  

E-print Network

Taiwan Nantou County earthquake 20130327 1 #12;0327 Taiwan Nantou County earthquake Source, Intensity 5 #12;I II III IV V VI VII Intensity Shake map of the March 27 Earthquake The peak ground and Technology Center for Disaster Reduction (NCDR) #12;Earthquake Response and Evacuation are a Part of Students

205

pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen  

SciTech Connect

Highlights: Black-Right-Pointing-Pointer ppGalNAc-T13 was up-regulated in high metastatic sublines of Lewis lung cancer. Black-Right-Pointing-Pointer ppGalNAc-T13 expression enhanced cell invasion activity in low metastatic sublines. Black-Right-Pointing-Pointer Trimeric Tn antigen was induced in the transfectant cells of ppGalNAc-T13 cDNA. Black-Right-Pointing-Pointer A major protein carrying trimeric Tn structure was identified as Syndecan-1. Black-Right-Pointing-Pointer Silencing of ppGalNAc-T13 resulted in the reduction of invasion and of metastasis.. -- Abstract: In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. These sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated by subcutaneous injection of T13-KD clones showed lower coalescence to fascia and peritoneum, and significantly reduced lung metastasis than control clones. These data suggested that high expression of pp-GalNAc-T13 gene generated trimeric Tn antigen on Syndecan-1, leading to the enhanced metastasis.

Matsumoto, Yasuyuki; Zhang, Qing [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Akita, Kaoru; Nakada, Hiroshi [Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-ku, Kyoto 603-8555 (Japan)] [Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-ku, Kyoto 603-8555 (Japan); Hamamura, Kazunori; Tokuda, Noriyo [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Tsuchida, Akiko [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan) [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Noguchi Institute, 1-8-1 Kaga, Itabashi, Tokyo 173-0003 (Japan); Matsubara, Takeshi; Hori, Tomoko; Okajima, Tetsuya [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Furukawa, Keiko [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan) [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, 1200 Matsumoto-cho, Kasugai 487-8501 (Japan); Urano, Takeshi [Department of Biochemistry, Shimane University School of Medicine, Izumo 693-8501 (Japan)] [Department of Biochemistry, Shimane University School of Medicine, Izumo 693-8501 (Japan); Furukawa, Koichi, E-mail: koichi@med.nagoya-u.ac.jp [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)] [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)

2012-03-02

206

7 CFR 718.8 - Administrative county.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 false Administrative county. 718.8 Section 718.8 Agriculture...Provisions 718.8 Administrative county. (a) If all land on the farm is physically located in one county, the farm shall be administratively...

2010-01-01

207

7 CFR 718.8 - Administrative county.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 false Administrative county. 718.8 Section 718.8 Agriculture...Provisions 718.8 Administrative county. (a) If all land on the farm is physically located in one county, the farm shall be administratively...

2012-01-01

208

7 CFR 718.8 - Administrative county.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 false Administrative county. 718.8 Section 718.8 Agriculture...Provisions 718.8 Administrative county. (a) If all land on the farm is physically located in one county, the farm shall be administratively...

2011-01-01

209

Hospital Development Guide for King County.  

National Technical Information Service (NTIS)

A hospital development guide for King County, Washington is described that involved a cooperative venture of the King County Comprehensive Health Planning Council, Puget Sound Health Planning Council, Seattle Area Hospital Council, and the King County Med...

1973-01-01

210

Frederick County Community Perception Survey.  

ERIC Educational Resources Information Center

In 1997, Frederick Community College (FCC) in Maryland conducted a telephone survey of a random sample of 466 Frederick County residents to identify their perceptions of the college. In particular, the survey examined Frederick County residents' image of FCC, level of awareness of services and programs offered by FCC, and the types of services

Frederick Community Coll., MD.

211

Equity and County College Financing.  

ERIC Educational Resources Information Center

An analysis by the Commission on Financial Postsecondary Education of the system by which New Jersey supports county colleges revealed disparities and inequities among the New Jersey counties in wealth and effort per full-time equivalent student, and current state financing practices that aggravate this problem. In response, alternative state

New Jersey State Commission on Financing Postsecondary Education, Trenton.

212

Explosive Demolition of a Fire-Water Tower At East Tennessee Technology Park, Oak Ridge TN  

SciTech Connect

On June 17, 2006, the Department of Energy (DOE) successfully demolished a {approx}60 year old fire-water tower (K-1206-E), located at the East Tennessee Technology Park (ETTP) in Oak Ridge, TN, using strategically placed explosive charges. The subject demolition project was executed by MCM Management Corporation and Demolition Dynamics under the management of DoE's prime contractor Bechtel Jacobs Company LLC (BJC). The K-1206-E Fire Water Tower (Tower) supported the ETTP fire water protection system from the mid- 1950's until 1991. The 378,500-L (100,000-gallon) Tower, elevated 53-m (175-feet) above grade, was located in a grassy area within 152-m (500-feet) of several other occupied facilities. Electrical, control circuits and supply water servicing the Tower were deactivated in 2003. Free liquids and sludge were removed from the tank prior to demolition. Demolition of a facility employing explosive demolition at a federal site in the 'post-9/11 era' was a substantial challenge. The subject paper discusses: - the planning and coordination steps that were taken to successfully overcome the challenges prior to the demolition of the empty, deactivated Tower; - the method used for the engineered demolition of the Tower; and - the factors responsible for the successful execution of this demolition project. At least two previous attempts were made to demolish the Tower. In the first attempt, the execution of the project was deferred by the re-allocation of funds. In the subsequent attempt in 2004, the execution of this project was postponed due to concerns that an adjacent facility would have to shut down operations during the duration of mobilization and execution of the project and thereby incur potential financial losses. A total of 51 cubic meters (1,800 cubic feet) of demolition debris was generated, which was compliantly disposed of at a local landfill followed by site restoration.

Brooksbank, R.D.; Rood, M.S.; Amrit, S.K.; Harper, M.S.; Dypolt, D.J.; Brehse, Mike [Bechtel Jacobs Company LLC, P.O. Box 4699 Oak Ridge, TN 37931 (United States)

2008-01-15

213

Terrestrial and Aquatic Controls on Watershed Biogeochemistry Respond Differently to Climate Change: Walker Branch, TN  

NASA Astrophysics Data System (ADS)

Most long-term watershed monitoring sites focus on catchments with shallow soils, consolidated bedrock, and small groundwater volumes such that hydrologic budgets can be accurately quantified. These characteristics make it difficult to disentangle the terrestrial and aquatic processes that govern biogeochemical cycles by limiting the pathways that water can be routed through watersheds. We analyzed 20 years of weekly streamwater chemistry data for the Walker Branch watershed (TN, USA), a site with exceptionally deep soils, complex soil flowpath structure, and large groundwater volumes that feed perennial springs. We show that long-term trends in climate patterns have altered catchment flowpath structure, such that vegetation and surface soils are becoming increasingly hydrologically isolated from the stream channel and that deep groundwater comprises a greater proportion of streamflow. Groundwater is chemically distinct from water that passes through shallow soils and, thus, changing flowpath contributions have resulted in long-term trends in streamwater solute concentrations and fluxes. Groundwater also exhibits little variation in solute concentrations across seasons and years, providing an ideal backdrop against which aquatic processes can be assessed. Aquatic processes in Walker Branch can have important effects on streamwater biogeochemistry during baseflow, but are sensitive to hydrologic disturbance and storm frequency. In addition to the long-term restructuring of soil flowpaths affecting terrestrial controls on biogeochemical patterns, changes in the frequency and intensity of storms have had distinct effects on aquatic controls governing solute concentrations and fluxes. The characteristics of Walker Branch that are generally considered undesirable for constructing water budgets and which set it apart from most other long-term watershed monitoring sitesdeep soils with complex flowpath structuring and large groundwater volumesallow us to disentangle the effects of upland vs. in-stream processes.

Lutz, B. D.; Bernhardt, E. S.

2012-12-01

214

County mapping of injury mortality.  

PubMed

Death rates for injuries were mapped by county in order to identify high-risk geographic areas. Overall rates of unintentional injury death were high in rural areas, especially in the West, and in low-income southern counties. Homicides, primarily due to firearms, had high rates throughout the South; elsewhere, homicide rates were high in large cities but not in suburban counties. Southern counties also had high rates of housefire mortality. Drowning rates for young children were high on the West Coast and in Florida, and were high for the population as a whole in the Mississippi Delta. Mapping by county is a potentially powerful tool for identifying high-risk areas and developing preventive measures. PMID:3385814

Baker, S P; Whitfield, R A; O'Neill, B

1988-06-01

215

Nucleotide sequence of insertion sequence IS3411, which flanks the citrate utilization determinant of transposon Tn3411.  

PubMed Central

The nucleotide sequences of insertion sequences IS3411L (left) and IS3411R (right), present as direct terminal repeats in the citrate utilization of citrate utilization transposon Tn3411, and of IS3411 (generated by intramolecular recombination between IS3411L and IS3411R) were determined. The three IS3411 elements (IS3411R, IS3411L, and IS3411) were 1,309 base pairs long and identical in DNA sequence. IS3411 had 27-base-pair terminal inverted repeats with three bases mismatched and one long open reading frame (240 amino acids) that was proposed to be a transposase. Three polypeptides of 29,000, 27,000, and about 10,000 molecular weight, determined by IS3411, were identified in minicells. Since Tn3411 generates a 3-base-pair repeat upon integration, the nucleotide sequences of IS3411 were compared with those of IS3. Images PMID:2832386

Ishiguro, N; Sato, G

1988-01-01

216

Surface modifications in the platelets of a patient with alpha-N-acetyl-D-galactosamine residues, the Tn-syndrome.  

PubMed Central

The Tn-syndrome is an acquired disorder characterized by the polyagglutination of blood cells and the pathological exposure of alpha-N-acetyl-D-galactosamine residues (Tn-antigen) at the cell surface. We now report studies on the platelet of a patient (Ba.) of which 81% reacted positively with a fluorescein conjugate of Helix pomatia agglutinin (HPA). The surface proteins of Ba. platelets were labeled with 125I by the lactoperoxidase-catalyzed procedure; single and two-dimensional electrophoresis on sodium dodecyl sulfate (SDS)-polyacrylamide gels was followed by autoradiography that revealed normal 125I-labeling of the major membrane glycoproteins (GP) but that GP Ib had a faster than normal migration. the abnormal GP Ib of Ba. platelets was strongly labeled when platelet suspensions were treated sequentially with neuraminidase, galactose oxidase, and sodium [3H]borohydride. Unlike the GP Ib of normal human platelets, it was also strongly labeled when Ba. platelets were treated with galactose oxidase and sodium [3H]borohydride alone. Both the alloantigen, PlA1, and quinidine-dependent antibody receptor activity were normally expressed by Ba. platelets, which also bound a monoclonal antibody (AN51) to GP Ib. Analysis of Ba. platelets by crossed immunoelectrophoresis using a rabbit anti-human platelet antibody preparation revealed the presence of an immunoprecipitate in the GP Ib position that had an abnormal appearance and migration in the second dimension. An altered position of the precipitate given by Factor VIIIR:Ag was also noted. Incorporation of HPA into the agarose gel during the first dimension electrophoresis resulted in the specific precipitation of the abnormal GP Ib of Ba. platelets. Our studies show that circulating Tn-platelets contain GP Ib with a modified oligosaccharide chain structure responsible for the platelet expression of Tn-antigen activity. Images PMID:7174794

Nurden, A T; Dupuis, D; Pidard, D; Kieffer, N; Kunicki, T J; Cartron, J P

1982-01-01

217

Expression of sialyl-Tn predicts the effect of adjuvant chemotherapy in node-positive breast cancer  

Microsoft Academic Search

Sialyl-Tn (STn) is a carcinoma-associated carbohydrate determinant expressed on cancer-associated mucins and has the structure NANA alpha(2-6)alpha GalNAc. Expression of STn in colon and ovarian cancer is associated with a poor prognosis independent of tumour grade, stage or histological type. We have examined 237 cases of primary breast cancer for expression of this antigen using the antibody HB-STn (Dako). The

DW Miles; LC Happerfield; P Smith; R Gillibrand; LG Bobrow; WM Gregory

1994-01-01

218

Precise Insertion of Antibiotic Resistance Determinants into Tn21Like Transposons: Nucleotide Sequence of the OXA-1 beta -lactamase Gene  

Microsoft Academic Search

Several plasmid-encoded beta -lactamase are on multiresistance transposable elements. The OXA-1 beta -lactamase gene is part of Tn2603, which is borne on the R plasmid RGN238. We report here the complete nucleotide sequence of the OXA-1 beta -lactamase gene and flanking sequences. The OXA-1 gene shows a >50% sequence divergence from the OXA-2 gene, yet there is significant functional similarity

Marc Ouellette; Luc Bissonnette; Paul H. Roy

1987-01-01

219

Analysis for prevalence and physical linkages amongst integrons, ISEcp1, ISCR1, Tn21 and Tn7 encountered in Escherichia coli strains from hospitalized and non-hospitalized patients in Kenya during a 19-year period (1992-2011)  

PubMed Central

Background We determined the prevalence and evidence for physical linkage amongst integrons, insertion sequences, Tn21 and Tn7 transposons in a collection of 1327 E. coli obtained over a 19-year period from patients in Kenya. Results The prevalence of class 1 integrons was 35%, class 2 integrons were detected in 3 isolates but no isolate contained a class 3 integron. Integron lacking the 3-CS or those linked to sul3 gene or IS26 or those containing the ISCR1 were only detected in multidrug resistant (MDR) strains. The dfrAs were the most common cassettes and their prevalence was: - dfrA1(28%), dfrA12(20%), dfA17(9%), dfrA7(9%), and dfrA16(5%). The aadA were the second most abundant cassettes and their prevalence was: - aadA1(25%), aadA2(21%), and aadA5(14%). Other cassettes occurred in lower prevalence of below 5%. Prevalence of Tn21, ISEcp1, ISCR1 and IS26 was 22%, 10%, 15%, and 7% respectively. Majority of Tn21 containing integrons carried a complete set of transposition genes while class 2 integrons were borne on Tn7 transposon. The qnrA genes were detected in 34(3%) isolates while 19(1%) carried qnrB. All qnr genes were in MDR strains carrying integrons containing the ISCR1. Close to 88% of blaTEM-52 were linked to IS26 while???80% of blaCTX-Ms and blaCMYs were linked to ISEcp1. Only a few studies have identified a blaCTX-M-9 containing an ISEcp1 element as reported in this study. Multiple genetic elements, especially those borne on incIl, incFII, and incL/M plasmids, and their associated resistance genes were transferrable en bloc to E. coli strain J53 in mating experiments. Conclusions This is the first detailed study on the prevalence of selected elements implicated in evolution of resistance determinants in a large collection of clinical E. coli in Africa. Proliferation of such strains carrying multiple resistance elements is likely to compromise the use of affordable and available treatment options for majority of poor patients in Africa. There is therefore a need to monitor the spread of these highly resistant strains in developing countries through proper infection control and appropriate use of antimicrobials. PMID:23682924

2013-01-01

220

Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.  

PubMed Central

The mini-Tn10 transposon (delta 16 delta 17Tn10) confers tetracycline resistance. When inserted between a gene and its promoter, it blocks transcription and prevents expression of that gene. Tetracycline in the medium induces divergent transcription of the tetA and tetR genes within the transposon, and this transcription extends beyond the transposon in both directions into the bacterial genes. If the mini-Tn10 inserts between an essential bacterial gene and its promoter, the insertion mutation can cause conditional growth which is dependent on the presence of tetracycline. Two essential genes in adjacent operons of Escherichia coli have been detected by screening for tetracycline dependence among tetracycline-resistant insertion mutants. These essential genes are the era gene in the rnc operon and the dpj gene in the adjacent pdxJ operon. The pdxJ operon has not been described previously. It consists of two genes, pdxJ and dpj. Whereas the dpj gene is essential for E. coli growth in all media tested, pdxJ is not essential. The pdxJ gene encodes a protein required in the biosynthesis of pyridoxine (vitamin B6). Images PMID:1537799

Takiff, H E; Baker, T; Copeland, T; Chen, S M; Court, D L

1992-01-01

221

Suppression of autolysis and cell wall turnover in heterogeneous Tn551 mutants of a methicillin-resistant Staphylococcus aureus strain.  

PubMed Central

Isogenic Tn551 mutants of a highly and uniformly methicillin-resistant strain of Staphylococcus aureus were tested for their rates of autolysis and cell wall degradation in buffer and for cell wall turnover during growth. The normal (relatively fast) autolysis and turnover rates of the parent strain were retained in a Tn551 mutant in which the insert was located within the mec gene and which produced undetectable levels of penicillin-binding protein 2A. On the other hand, autolysis and cell wall turnover rates were greatly reduced in auxiliary mutants, i.e., mutants in which the transposon caused conversion of the high-level and uniform resistance of the parent strain to a variety of distinct heterogeneous expression types and greatly decreased resistance levels. All of these mutants contained an intact mec gene and produced normal amounts of penicillin-binding protein 2A, and one of the mutations was located in the femA region of the staphylococcal chromosome (B. Berger-Bachi, L. Barberis-Maino, A. Strassle, and F. H. Kayser, Mol. Gen. Genet. 219:263-269, 1989). Autolysis rates were related to the degree of residual methicillin resistance and to the sites of Tn551 insertion. Fast cell wall turnover may help expression of high-level methicillin resistance by providing a mechanism for the excision of abnormal (and potentially lethal) structural elements of the cell wall synthesized by the bacteria in the presence of methicillin. Images PMID:1846855

de Jonge, B L; de Lencastre, H; Tomasz, A

1991-01-01

222

Testing and analyses of the TN-24P PWR spent-fuel dry storage cask loaded with consolidated fuel  

SciTech Connect

A performance test of a Transnuclear, Inc. TN-24P storage cask configured for pressurized water reactor (PWR) spent fuel was performed. The work was performed by the Pacific Northwest Laboratory (PNL) and Idaho National Engineering Laboratory (INEL) for the US Department of Energy Office of Civilian Radioactive Waste Management (OCRWM) and the Electric Power Research Institute. The performance test consisted of loading the TN-24P cask with 24 canisters of consolidated PWR spent fuel from Virginia Power's Surry and Florida Power and Light's Turkey Point reactors. Cask surface and fuel canister guide tube temperatures were measured, as were cask surface gamma and neutron dose rates. Testing was performed with vacuum, nitrogen, and helium backfill environments in both vertical and horizontal cask orientations. Transnuclear, Inc., arranged to have a partially insulated run added to the end of the test to simulate impact limiters. Limited spent fuel integrity data were also obtained. From both heat transfer and shielding perspectives, the TN-24P cask with minor refinements can be effectively implemented at reactor sites and central storage facilities for safe storage of unconsolidated and consolidated spent fuel. 35 refs., 93 figs., 17 tabs.

McKinnon, M A; Michener, T E; Jensen, M F; Rodman, G R

1989-02-01

223

The Corynebacterium xerosisComposite Transposon Tn 5432Consists of Two Identical Insertion Sequences, Designated IS 1249,Flanking the Erythromycin Resistance Gene ermCX  

Microsoft Academic Search

Analysis of the 50-kb R-plasmid pTP10 from the clinical isolateCorynebacterium xerosisM82B revealed that the erythromycin resistance gene,ermCX,is located on a 4524-bp composite transposable element, Tn5432.The ends of Tn5432are identical, direct repeats of an insertion sequence, designated IS1249,encoding a putative transposase of the IS256family. IS1249consists of 1385 bp with 45\\/42 imperfect terminal inverted repeats. The nucleotide sequence of the 1754-bp Tn5432central

ANDREAS TAUCH; FRIEDRICH KASSING; JRN KALINOWSKI; ALFRED PHLER

1995-01-01

224

First results from TN273 studies of the SE Mariana Forearc rift  

NASA Astrophysics Data System (ADS)

TN 273 aboard R/V Thomas Thompson (Dec. 22 2011- Jan. 22 2012) studied an unusual region of rifting affecting the southern Mariana forearc S.W. of Guam. The S.E. Mariana Forearc Rift (SEMFR) formed by diffuse tectonic and volcanic deformation (Martinez and Sleeper, this meeting) ~2.7-3.7 Ma ago to accommodate opening of the southernmost Mariana Trough backarc basin. A total of 730 km linear-track of SEMFR seafloor was surveyed with deep-towed side-scan sonar IMI-30. 14 dredges provided samples of SEMFR igneous rocks, analyzed for whole rock (WR) and glass compositions. These new results coupled with results of earlier investigations confirm that SEMFR is dominated by Miocene lavas along with minor gabbro and diabase. SEMFR lavas range in major element composition from primitive basalt to fractionated andesite (Mg# = 0.36-0.73; SiO2 = 50-57 wt%), mainly controlled by crystal fractionation. Rare Earth Element (REE) patterns range from LREE-depleted, N-MORB-like to flat patterns, reflecting different mantle processes (i.e. different sources, degree of melting ). Glassy rinds and olivine-hosted melt inclusions in these lavas contain variable volatile compositions (F = 75-358 ppm, S = 35-1126 ppm, Cl= 74-1400 ppm, CO2 = 15-520 ppm, 0.36-2.36 wt% H2O). SEMFR lavas show spider diagrams with positive anomalies in LILE and negative anomalies in HSFE. SEMFR lavas have backarc basin-like (BAB-like) chemical composition (H2O < 2.5wt%, Ba/Yb~20, Nb/Yb~1 and ?Nd~9) along with stronger enrichment in Rb and Cs than arc and BAB lavas, as demonstrated by their higher Rb/Th and Cs/Ba ratios in WR and glasses, which may reflect the role of the ultra-shallow fluids. Ultra-shallow fluids are derived from the top of the subducting slab, beneath the forearc, where most of the water and the fluid-mobile elements (Rb, Cs, Ba,) are thought to be released (Schmidt and Poli, 1998, EPSL, Savov et al., 2005, G-3). Our results suggest that i) SEMFR lavas formed by metasomatism of a BAB mantle source by ultra-shallow fluids, likely released from subducted sediments and the altered oceanic crust; and ii) the ultra-shallow fluid is aqueous and is characterized by enrichment in Cs and Rb, suggesting that Cs and Rb are decoupled from Ba in ultra-shallow subduction processes.

Ribeiro, J. M.; Stern, R. J.; Kelley, K. A.; Shaw, A. M.; Shimizu, N.; Martinez, F.; Ishii, T.; Ishizuka, O.; Manton, W. I.

2012-12-01

225

Tennessee Higher Education County Profiles, 2008  

ERIC Educational Resources Information Center

This report presents a localized perspective on Tennessee higher education, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; (3) number of county residents enrolled in Tennessee public institutions; and (4) number of county residents participating in the

Tennessee Higher Education Commission, 2008

2008-01-01

226

Physical Inactivity Estimates, by County  

MedlinePLUS

... Estimates, by County Share Compartir Staying active during leisure time can reduce your risk of obesity, type ... mental health. New estimates of physical inactivity during leisure time are available from CDC, and include estimates ...

227

Michigan County Histories and Atlases  

NSDL National Science Digital Library

How does one tell the story of a county? It's a tough task, but the staff members at the University of Michigan's Library system worked with a range of partners (including the Michigan Council of Library Directors) to make these county histories widely accessible. On this site, visitors can look over 428 digitized titles that tell the history of the Wolverine State. Interested parties can perform keyword searches across all of these volumes or just browse around at their leisure. First-time visitors might do well to look at the "Account of Kalamazoo County" from 1928 and the 1900 tome "An account of Southwest Michigan and Calhoun County." Additionally, the site contains business directories for a number of communities across the state. These documents are a great way to learn about the retail and other commercial activities going on from the 19th to the early 20th centuries. [KMG

2007-10-19

228

VERMONT COUNTY HEALTH DATA2  

EPA Science Inventory

This datalayer contains Vermont Population and Health data describing public health (1986-2000), by county, extracted from various sources, such as; the Vermont Department of Health, the Vermont Center for Justice Research, the National Institute on Alcohol Abuse & Alcoholis...

229

Fault Trace: Marin County, California  

NSDL National Science Digital Library

This photograph shows the trace of a fault (in trench phase) as it passes beneath a barn. The trace developed during the April 18, 1906 San Francisco Earthquake. The location is the Skinner Ranch, near Olema, Marin County, California.

230

Geothermal Development Plan: Pima County  

SciTech Connect

Pima County is located entirely within the Basin and Range physiographic province in which geothermal resources are known to occur. Continued growth as indicated by such factors as population growth, employment and income will require large amounts of energy. It is believed that geothermal energy could provide some of the energy that will be needed. Potential users of geothermal energy within the county are identified.

White, D.H.

1981-01-01

231

Single Resting hsTnT Level Predicts Abnormal Myocardial Stress Test in Acute Chest Pain Patients With Normal Initial Standard Troponin  

PubMed Central

OBJECTIVES The goal of this study was to determine the ability of a single, resting high-sensitivity troponin T (hsTnT) measurement to predict abnormal myocardial perfusion imaging (MPI) in patients presenting with acute chest pain to the emergency department (ED). BACKGROUND HsTnT assays precisely detect very low levels of troponin T, which may be a surrogate for the presence and extent of myocardial ischemia. METHODS We included all patients from the ROMICAT I (Rule Out Myocardial Infarction Using Computer Assisted Tomography) trial, an observational cohort study, who underwent both single-photon emission computed tomography (SPECT)-MPI stress testing and 64-slice computed tomography angiography (CTA) and in whom hsTnT measurements were available. We assessed the discriminatory value of hsTnT for abnormal SPECT-MPI and the association of reversible myocardial ischemia by SPECT-MPI and the extent of coronary atherosclerosis by CTA to hsTnT levels. RESULTS Of the 138 patients (mean age 54 11 years, 46% male), 19 (13.7%) had abnormal SPECT-MPI. Median hsTnT levels were significantly different between patients with normal and abnormal SPECT-MPI (9.41 pg/ml [interquartile range (IQR): 5.73 to 19.20 pg/ml] vs. 4.89 pg/ml [IQR: 2.34 to 7.68 pg/ml], p = 0.001). Sensitivity of 80% and 90% to detect abnormal SPECT-MPI was reached at hsTnT levels as low as 5.73 and 4.26 pg/ml, respectively. Corresponding specificity was 62% and 46%, and negative predictive value was 96% and 96%, respectively. HsTnT levels had good discriminatory ability for prediction of abnormal SPECT-MPI (area under the curve: 0.739, 95% confidence interval: 0.609 to 0.868). Both reversible myocardial ischemia and the extent of coronary atherosclerosis (combined model r2 = 0.19 with partial of r2 = 0.12 and r2 = 0.05, respectively) independently and incrementally predicted the measured hsTnT levels. CONCLUSIONS In patients with acute chest pain, myocardial perfusion abnormalities and coronary artery disease are predicted by resting hsTnT levels. Prospective evaluations are warranted to confirm whether resting hsTnT could serve as a powerful triage tool in chest pain patients in the ED before diagnostic testing and improve the effectiveness of patient management. PMID:23328564

Ahmed, Waleed; Schlett, Christopher L.; Uthamalingam, Shanmugam; Truong, Quynh A.; Koenig, Wolfgang; Rogers, Ian S.; Blankstein, Ron; Nagurney, John T.; Tawakol, Ahmed; Januzzi, James L.; Hoffmann, Udo

2013-01-01

232

Response of high-risk of recurrence/progression bladder tumours expressing sialyl-Tn and sialyl-6-T to BCG immunotherapy  

PubMed Central

Background: High risk of recurrence/progression bladder tumours is treated with Bacillus Calmette-Gurin (BCG) immunotherapy after complete resection of the tumour. Approximately 75% of these tumours express the uncommon carbohydrate antigen sialyl-Tn (Tn), a surrogate biomarker of tumour aggressiveness. Such changes in the glycosylation of cell-surface proteins influence tumour microenvironment and immune responses that may modulate treatment outcome and the course of disease. The aim of this work is to determine the efficiency of BCG immunotherapy against tumours expressing sTn and sTn-related antigen sialyl-6-T (s6T). Methods: In a retrospective design, 94 tumours from patients treated with BCG were screened for sTn and s6T expression. In vitro studies were conducted to determine the interaction of BCG with high-grade bladder cancer cell line overexpressing sTn. Results: From the 94 cases evaluated, 36 had recurrence after BCG treatment (38.3%). Treatment outcome was influenced by age over 65 years (HR=2.668; (1.3445.254); P=0.005), maintenance schedule (HR=0.480; (0.2460.936); P=0.031) and multifocallity (HR=2.065; (1.0334.126); P=0.040). sTn or s6T expression was associated with BCG response (P=0.024; P<0.0001) and with increased recurrence-free survival (P=0.001). Multivariate analyses showed that sTn and/or s6T were independent predictive markers of recurrence after BCG immunotherapy (HR=0.296; (0.1480.594); P=0.001). In vitro studies demonstrated higher adhesion and internalisation of the bacillus to cells expressing sTn, promoting cell death. Conclusion: s6T is described for the first time in bladder tumours. Our data strongly suggest that BCG immunotherapy is efficient against sTn- and s6T-positive tumours. Furthermore, sTn and s6T expression are independent predictive markers of BCG treatment response and may be useful in the identification of patients who could benefit more from this immunotherapy. PMID:24064971

Lima, L; Severino, P F; Silva, M; Miranda, A; Tavares, A; Pereira, S; Fernandes, E; Cruz, R; Amaro, T; Reis, C A; Dall'Olio, F; Amado, F; Videira, P A; Santos, L; Ferreira, J A

2013-01-01

233

77 FR 44268 - National Register of Historic Places; Notification of Pending Nominations and Related Actions  

Federal Register 2010, 2011, 2012, 2013

...Bryson City, 12000515 SOUTH DAKOTA Lawrence County Pearson Cabin, \\1/3\\ mi. SE. of jct. of Radio Rd. & US 14, Deadwood, 12000514 TENNESSEE Sevier County Clingmans Dome Observation Tower, Terminus of Clingmans Dome Rd., Bryson City,...

2012-07-27

234

Copyright0 1987 by the GeneticsSocietyof America A Tn10-lacZ-kanR-URA3Gene Fusion Transposon for Insertion  

E-print Network

Copyright0 1987 by the GeneticsSocietyof America A Tn10-lacZ-kanR-URA3Gene Fusion Transposon or translation start signals,an intact URA3 gene, and a RanR determinant. The lacZ gene can be activated be substituted for URA3 in the availableconstructs. The mini-Tn 10-LUKsystem has several important advantages. (1

Botstein, David

235

Mobilization of Escherichia coli R1 silver-resistance plasmid pJT1 by Tn5Mob into Escherichia coli C600  

Microsoft Academic Search

Summary Escherichia coli Rl is an Ag+-resistant strain that, as we have shown recently, harbours at least two large plasmids, pJT1 (83 kb) and pJT2 (77 kb). Tn5-Mob was introduced into theE. coli Rl host replicon via conjugation on membrane filters. The transfer functions of plasmid RP4-4 were provided in this process and Tn5-Mob clones mated withE. coli C600 yielded

Mary-Ellen Starodub; Jack T. Trevors

1990-01-01

236

Elution of Nitrate at the NABIR Field Research Center, Oak Ridge Reservation, Oak Ridge, TN  

NASA Astrophysics Data System (ADS)

As part of a bioremediation project for the in situ bioreduction of uranium at the Department of Energy Natural and Accelerated Bioremediation Research (NABIR) Field Research Center (FRC) in Oak Ridge, TN, aquifer and groundwater conditioning is required before conducting the remediation experiment. One step includes flushing of the aquifer with pH-adjusted fresh water in order to remove extremely high concentrations of nitrate, calcium, and aluminum that would interfere with in situ bioreduction. The elution of nitrate from the test zone was used as an inverse tracer to discern contaminant transport pathways and model parameters. Concentration time series data augmented pressure tests, a bromide tracer study, and electromagnetic borehole flowmeter (EBF) measurements. The aquifer at the FRC is a fractured shale with strike of about 1.5 degrees north of west, and dip of about 30 degrees to the southwest, as inferred from area observations and EBF logging. A network of injection and extraction wells are aligned along strike, while a separate network of observation wells with multiple screen intervals (MLS wells) are oriented along dip at the midpoint of the injection/extraction well network. Flow generally occurs along strike in fractures associated with bedding planes, however other lesser fracture networks provide communication between the major fracture sets. Previous data have indicated a high hydraulic conductivity zone, approximately 10-50 cm thick located at a depth of about 12 m along the centerline of the injection/extraction well network. Above the major flow zone, the matrix weathers to saprolite, decreasing hydraulic conductivity. The elution tracer test was conducted by injecting clean, acidified tap water in the farthest upgradient injection well, and extracting at half the injection rate from the farthest downgradient extraction well. This flow ratio was chosen to produce flushing focused on a small cell of the aquifer which will later be used as an in situ bioreactor. Nitrate concentrations were measured frequently over 100 hours. Concentration time series indicate that flushing occurred predominantly in the previously identified high conductivity zone at 12 m depth. The strongest, most rapid nitrate concentration decrease was observed at a similar depth in each MLS well, which is consistent with the depth at which injection and extraction occurred. A decrease in downdip, nitrate concentration extended to 15m depth, whereas at the centerline and updip, little nitrate concentration reduction occurred below 13.75 m depth. This asymmetry is consistent with the competing contributions of bedding plane fractures and weathering depths. In the future, time series crosshole seismic and ground penetrating radar collected by Lawrence Berkeley National Laboratory, and borehole electrical conductivity measurements collected by Oak Ridge National Laboratory will be integrated with the concentration, pressure, and EBF data to obtain a comprehensive interpretation of this test.

Fienen, M. N.; Criddle, C. S.; Jardine, P. M.; Kitanidis, P. K.; Mehlhorn, T. L.; Watson, D. B.; Wu, W.

2003-12-01

237

Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition  

SciTech Connect

Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZ{alpha}-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cells as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.

Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Matsuura, Masaaki [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University (Japan); Gomi, Yasuyuki [Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University (Japan); Takahashi, Michiaki [Research Foundation for Microbial Diseases of Osaka University (Japan); Yamanishi, Koichi [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.j [Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation (Japan); Division of Clinical Virology, Kobe University Graduate School of Medicine (Japan)

2010-06-20

238

A novel structure of Tn4001-truncated element, type V, in clinical enterococcal isolates and multiplex PCR for detecting aminoglycoside resistance genes.  

PubMed

A multiplex polymerase chain reaction (PCR) was established for detecting aacA-aphD, aph(2'')-Ib, aph(2'')-Ic and aph(2'')-Id, encoding high-level gentamicin resistance (HLGR), and aadA and aadE, encoding high-level streptomycin resistance (HLSR), in enterococci. The assay was implemented for 419 enterococcal blood and urine isolates recovered from patients at a university hospital in Thailand. Among the isolates tested, 56.1% (235 isolates) and 58.9% (247 isolates) contained aacA-aphD and aadE, respectively. The aph(2'')-Ib, aph(2'')-Ic, aph(2'')-Id and aadA genes were not found in any isolate. Among the isolates carrying the aacA-aphD gene, 99.1% exhibited a HLGR phenotype. All 235 enterococcal isolates containing aacA-aphD were further studied by PCR to characterise the structure of the resistance determinants carrying the aacA-aphD gene. The result revealed that only 22.6% carried Tn4001-related element, whereas the remaining isolates contained Tn4001-truncated element. No Tn4001-IS257 hybrid structure was detected. The majority of isolates carrying Tn4001-related element were Enterococcus faecalis (77.4%). Among Tn4001-truncated elements detected, all previously reported types (types I-IV) were found. Furthermore, a novel Tn4001-truncated type, designated type V, was also identified. PMID:18155882

Leelaporn, A; Yodkamol, K; Waywa, D; Pattanachaiwit, S

2008-03-01

239

Transposon Tn5281 is the main distributor of the aminoglycoside modifying enzyme gene among isolates of Enterococcus faecalis in Tehran hospitals.  

PubMed

Infections with high levels of gentamicin-resistant (HLGR) isolates of Enterococcus faecalis are common in Tehran hospitals. Genes encoding such resistance are transmissible by conjugation at high frequency. The purpose of this study was to determine the existence of Tn5281 and its flanking aminoglycoside modifying enzyme gene aac(6')-aph(2") among 102 HLGR isolates of E. faecalis cultured from patients at three hospitals in Tehran, Iran. These isolates were detected by disks containing 120 microg of gentamicin and made 65% of all E. faecalis during the study period. DNA was extracted from HLGR isolates and subjected to PCR assays targeting aac(6')-aph(2") and conjugative transposon Tn5281. The amplified aac(6')-aph(2") gene was labeled with digoxigenin and probed with Tn5281 amplicons in dot blot hybridization assays. The aac(6')-aph(2") gene was detected in 91%-92% (n = 93) of the HLGR isolates. All isolates containing aac(6')-aph(2") were positive in long-PCR targeting Tn5281 and the probe hybridized with Tn5281 amplicons. The number of HLGR isolates of E. faecalis has increased considerably in Tehran hospitals. Tn5281 is the main cause of transmission of aac(6')-aph(2") to different isolates of E. faecalis in the hospitals studied. PMID:18923558

Feizabadi, Mohammad Mehdi; Shokrzadeh, Leila; Sayady, Sara; Asadi, Soroor

2008-10-01

240

Rapid transposon liquid enrichment sequencing (TnLE-seq) for gene fitness evaluation in underdeveloped bacterial systems.  

PubMed

Whole-genome fitness analysis in microbes that uses saturating transposon mutagenesis combined with massively parallel sequencing (Tn-seq) is providing a measure of the contribution of each gene to a given growth condition. With this technique, gene fitness profiles and essential genes are discovered by simultaneous analyses of whether the absence of each gene product alters the growth kinetics of the bacterium. Here we modify the standard Tn-seq procedure to simplify and shorten the process by including delivery of the transposon through conjugation and liquid culture enrichment of the mutant pool, creating transposon liquid enrichment sequencing (TnLE-seq). To illustrate the success of these modifications and the robustness of the procedure, analyses of gene fitness of two cultures of the strictly anaerobic bacterium Desulfovibrio vulgaris Hildenborough were performed, with growth on lactate as the electron donor and sulfate as the electron acceptor. These data demonstrate reproducibility and provide a base condition for analysis of fitness changes in deletion mutants and in various growth conditions. The procedural modifications will facilitate the application of this powerful genetic analysis to microbes lacking a facile genetic system. Pilot studies produced 2.510(5) and 3.410(5) unique insertion mutants in the anaerobe Desulfovibrio vulgaris Hildenborough grown under typical laboratory conditions in rich medium. These analyses provided two similar high-resolution maps of gene fitness across the genome, and the method was also applied to growth in minimal medium. These results were also compared to the coverage obtained with a ca. 13,000-member cataloged transposon library constructed by sequencing transposon insertion sites in individual mutants. PMID:24077707

Fels, Samuel R; Zane, Grant M; Blake, Sean M; Wall, Judy D

2013-12-01

241

Rapid Transposon Liquid Enrichment Sequencing (TnLE-seq) for Gene Fitness Evaluation in Underdeveloped Bacterial Systems  

PubMed Central

Whole-genome fitness analysis in microbes that uses saturating transposon mutagenesis combined with massively parallel sequencing (Tn-seq) is providing a measure of the contribution of each gene to a given growth condition. With this technique, gene fitness profiles and essential genes are discovered by simultaneous analyses of whether the absence of each gene product alters the growth kinetics of the bacterium. Here we modify the standard Tn-seq procedure to simplify and shorten the process by including delivery of the transposon through conjugation and liquid culture enrichment of the mutant pool, creating transposon liquid enrichment sequencing (TnLE-seq). To illustrate the success of these modifications and the robustness of the procedure, analyses of gene fitness of two cultures of the strictly anaerobic bacterium Desulfovibrio vulgaris Hildenborough were performed, with growth on lactate as the electron donor and sulfate as the electron acceptor. These data demonstrate reproducibility and provide a base condition for analysis of fitness changes in deletion mutants and in various growth conditions. The procedural modifications will facilitate the application of this powerful genetic analysis to microbes lacking a facile genetic system. Pilot studies produced 2.5 105 and 3.4 105 unique insertion mutants in the anaerobe Desulfovibrio vulgaris Hildenborough grown under typical laboratory conditions in rich medium. These analyses provided two similar high-resolution maps of gene fitness across the genome, and the method was also applied to growth in minimal medium. These results were also compared to the coverage obtained with a ca. 13,000-member cataloged transposon library constructed by sequencing transposon insertion sites in individual mutants. PMID:24077707

Fels, Samuel R.; Zane, Grant M.; Blake, Sean M.

2013-01-01

242

Napa County Conservation,Napa County Conservation, Development & PlanningDevelopment & Planning  

E-print Network

Napa County Conservation,Napa County Conservation, Development & PlanningDevelopment & Planning DepartmentDepartment Watershed Information Center (WICC)Watershed Information Center (WICC) & Conservancy Board& Conservancy Board of Napa Countyof Napa County www.napawatersheds.org Watershed Information

243

75 FR 25308 - Environmental Impact Statement: Winnebago County, IL and Rock County, WI  

Federal Register 2010, 2011, 2012, 2013

...Administration Environmental Impact Statement: Winnebago County, IL and Rock County, WI AGENCY: Federal Highway Administration (FHWA...Wisconsin Route 213 and Nye School Road northwest of Beloit, Rock County, Wisconsin to the interchange of Rockton Road and...

2010-05-07

244

Mutagenesis of the metJBLF gene cluster with transposon Tn5: localization of the metF transcriptional unit  

E-print Network

LIST OF FIGURES PAGE 1. Utilization of Methionine in the Biosynthesis of S-adenosyl- Methionine (SAM) . 2. Biosynthetic Pathway of Methionine from Aspartate in Escherr'chia coli K1 2 3. The Recombination Map of Escherichia coli K12 4. The Genetic... of Recombinant Plasmids pTJ77H, pTJ3-1H, and pTJ77B 42 TABLE LIST OF TABLES PAGE 1. Escherichia coli K 12 Strains 16 2. Restricition Endonuclease-Fragment Size Analysis 32 3. Spot Transduction Patterns of Lambda dmeft 28::Tn5 Isolates . . . 36 4. Enzyme...

Treat, Mary Love

2012-06-07

245

S-Michael additions to chiral dehydroalanines as an entry to glycosylated cysteines and a sulfa-Tn antigen mimic.  

PubMed

Stereoselective sulfa-Michael addition of appropriately protected thiocarbohydrates to chiral dehydroalanines has been developed as a key step in the synthesis of biologically important cysteine derivatives, such as S-(?-D-glucopyranosyl)-D-cysteine, which has not been synthesized to date, and S-(2-acetamido-2-deoxy-?-D-galactopyranosyl)-L-cysteine, which could be considered as a mimic of Tn antigen. The corresponding diamide derivative was also synthesized and analyzed from a conformational viewpoint, and its bound state with a lectin was studied. PMID:24372047

Aydillo, Carlos; Compan, Ismael; Avenoza, Alberto; Busto, Jess H; Corzana, Francisco; Peregrina, Jess M; Zurbano, Mara M

2014-01-15

246

Population substructure in Cache County, Utah: the Cache County study  

PubMed Central

Background Population stratification is a key concern for genetic association analyses. In addition, extreme homogeneity of ethnic origins of a population can make it difficult to interpret how genetic associations in that population may translate into other populations. Here we have evaluated the genetic substructure of samples from the Cache County study relative to the HapMap Reference populations and data from the Alzheimer's Disease Neuroimaging Initiative (ADNI). Results Our findings show that the Cache County study is similar in ethnic diversity to the self-reported "Whites" in the ADNI sample and less homogenous than the HapMap CEU population. Conclusions We conclude that the Cache County study is genetically representative of the general European American population in the USA and is an appropriate population for conducting broadly applicable genetic studies. PMID:25078123

2014-01-01

247

Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi.  

PubMed

A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled by this episome (Lac+, Tetr, Tra+) were expressed, and its replication was stopped at 40 degrees C and above. At 30 degrees C, the episome was easily transferred between strains derived from E. carotovora subsp. chrysanthemi 3937j and to E coli. Hfr donor strains were obtained from a F' strain of 3937j by selecting clones which grew at 40 degrees C on plates containing tetracycline. One of these strains, Hfrq, was examined in more detail: the characters Lac+ and Tetr were stabilized and did not segregate higher than its parental F' strain. The mating was most efficient at 37 degrees C on a membrane. Hfrq transferred its chromosome to recipient strains at high frequency and in a polarized fashion, as evidenced by the gradient of transfer frequencies, the kinetics of marker entry (in interrupted mating experiments), and the analysis of linkage between different markers. The chromosome of Hfrq was most probably transferred in the following sequence: origin...met...xyl...arg...ile...leu...thr...cys...pan...ura...gal...trp...his. ..pur... Moreover, this genetic transfer system proved to be efficient in strain construction. PMID:6277860

Kotoujansky, A; Lemattre, M; Boistard, P

1982-04-01

248

Evidence for a disseminated erythromycin resistance determinant mediated by Tn917-like sequences among group D streptococci isolated from pigs, chickens, and humans.  

PubMed

A total of 199 streptococci isolated from feces of healthy chickens, pigs, and beef cattle and 26 human clinical isolates were tested for resistance to kanamycin, streptomycin, tetracycline, erythromycin, and lincomycin. Of 66 isolates resistant to these antibiotics, 12 transferred one or more resistance traits by conjugation in broth. Erythromycin resistance (Emr) was transferred from 10 of the 12 successful donors. AvaI digests of plasmids isolated from Emr transconjugants derived from two human, two chicken, and one pig isolate contained three fragments similar in size to those produced from Tn3871, an Emr transposon. The three fragments from each of the five digests on Southern blots hybridized to radiolabeled Tn3871. Plasmid DNA from a transconjugant derived from a second pig isolate contained two of the three Tn3871-associated AvaI fragments. One of the AvaI fragments from each of the six plasmids hybridized with a radiolabeled probe containing a cloned AvaI fragment from Tn3871 that contained the Emr determinant. Transposition of the Emr trait was demonstrated for the plasmids derived from one human and one pig isolate. We concluded that extensive DNA homology existed between plasmids from streptococcal strains obtained from two human patients, two chickens, and two pigs and the Emr transposon Tn3871, which is very similar or identical to the well-characterized Emr transposon Tn917. The detection of Tn3871-like sequences in streptococcal isolates from Arkansas, Illinois, North Carolina, and Washington, D.C. indicates wide dissemination of Emr mediated by the same or closely related transposons. PMID:2988428

Rollins, L D; Lee, L N; LeBlanc, D J

1985-04-01

249

(Dengue Fever reported in Osceola County) OSCEOLA COUNTY HEALTH DEPARTMENT  

E-print Network

mosquitoes can lay their eggs. DEET ­ When the potential exists for exposure to mosquitoes, repellents ADVISORY FOR MOSQUITO-BORNE DISEASE For Questions contact: Camille Bissainthe (407) 343-2001 Public Urged to Protect Against Mosquitoes and Mosquito-Borne Viruses KISSIMMEE -- The Osceola County Health Department

Watson, Craig A.

250

HaMiltonCounty Number of volunteers 114  

E-print Network

of beef cattle Giving Recent donors residing in county 124 FY 2012 donors residing in county 64 Gifts to UF from county residents $21,572 Gifts to IFAS from county residents $10,170 Funding State funds

Ma, Lena

251

gilCHristCounty Number of volunteers 461  

E-print Network

residing in county 663 FY 2012 donors residing in county 394 Gifts to UF from county residents $51,991 Gifts to IFAS from county residents $250 Funding State funds for Extension $221,611 Federal funds

Ma, Lena

252

MaDisonCounty Number of volunteers 157  

E-print Network

Giving Recent donors residing in county 218 FY 2012 donors residing in county 99 Gifts to UF from county residents $22,489 Gifts to IFAS from county residents $7,925 Funding State funds for Extension $332

Ma, Lena

253

33. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PLAN OF STONE PIER FOR EAST END, SUTLIFF'S FERRY BRIDGE, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

254

35. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. SPECIFICATIONS FOR BRIDGE OVER THE CEDAR RIVER AT SUTLIFF'S FERRY JOHNSON COUNTY, IOWA - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

255

7 CFR 7.10 - Conduct of county convention.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 2010-01-01 false Conduct of county convention. 7.10 Section 7.10...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.10 Conduct of county convention. (a) The county...

2010-01-01

256

7 CFR 7.11 - County committee members.  

Code of Federal Regulations, 2012 CFR

... 2012-01-01 2012-01-01 false County committee members. 7.11 Section 7...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.11 County committee members. (a) County...

2012-01-01

257

7 CFR 7.10 - Conduct of county convention.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 2011-01-01 false Conduct of county convention. 7.10 Section 7.10...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.10 Conduct of county convention. (a) The county...

2011-01-01

258

7 CFR 7.10 - Conduct of county convention.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 2012-01-01 false Conduct of county convention. 7.10 Section 7.10...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.10 Conduct of county convention. (a) The county...

2012-01-01

259

7 CFR 7.11 - County committee members.  

Code of Federal Regulations, 2011 CFR

... 2011-01-01 2011-01-01 false County committee members. 7.11 Section 7...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.11 County committee members. (a) County...

2011-01-01

260

7 CFR 7.11 - County committee members.  

Code of Federal Regulations, 2010 CFR

... 2010-01-01 2010-01-01 false County committee members. 7.11 Section 7...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.11 County committee members. (a) County...

2010-01-01

261

Efficient role of BacTN635 on the safety properties, sensory attributes, and texture profile of raw minced meat beef and chicken breast.  

PubMed

Bacteriocin BacTN635, produced by Lactobacillus plantarum sp. TN635, was purified and characterised in previous work. In this study we report the biotechnological application of this bacteriocin as a biopreservative during storage at 4C of raw minced meat beef and chicken breast. Overall, the results obtained showed that the addition of the semi-purified BacTN635 at 500 or 1000 AU g(-1) in raw minced meat beef and chicken breast can delay the proliferation of spoilage microorganisms, suppress the growth of the pathogenic microorganism Listeria monocytogenes, improve sensory quality, texture attributes, and extend the shelf-life of these two meat products during refrigerated storage. BacTN635 at 1000 AU g(-1) could extend the shelf-life, and the meat showed good sensory characteristics. Therefore, treatment with semi-purified BacTN635 can be used as a safe method for preservation of raw minced meat beef and chicken breast. PMID:24320082

Smaoui, S; Elleuch, L; Ben Salah, R; Najah, S; Chakchouk-Mtibaa, A; Sellem, I; Besbes, S; Mellouli, L

2014-01-01

262

The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX.  

PubMed

Analysis of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium xerosis M82B revealed that the erythromycin resistance gene, ermCX, is located on a 4524-bp composite transposable element, Tn5432. The ends of Tn5432 are identical, direct repeats of an insertion sequence, designated IS1249, encoding a putative transposase of the IS256 family. IS1249 consists of 1385 bp with 45/42 imperfect terminal inverted repeats. The nucleotide sequence of the 1754-bp Tn5432 central region is 99% identical to the previously sequenced erythromycin resistance region of the Corynebacterium diphtheriae plasmid pNG2. It encodes the erythromycin resistance gene, ermCX, and an ORF homologous to the amino-terminal end of the transposase of IS31831 from Corynebacterium glutamicum. Transposons with regions flanking the insertion sites were recovered from the C. glutamicum chromosome by a plasmid rescue technique. Insertion of Tn5432 created 8-bp target site duplications. A Tn5432-induced isoleucine/valine-auxotrophic mutant was found to carry the transposon in the 5' region of the ilvBNC cluster; in pTP10 the transposon is inserted in a region similar to replication and partitioning functions of the Enterococcus faecalis plasmid pAD1 and the Agrobacterium tumefaciens plasmid pTAR. PMID:8559800

Tauch, A; Kassing, F; Kalinowski, J; Phler, A

1995-09-01

263

Martin County Master Gardeners In cooperation with the Martin County Library System  

E-print Network

Martin County Master Gardeners In cooperation with the Martin County Library System Present Recycling in Martin County Monday, September 23, 2013 - 6:00 PM Blake Library, 2351 SE Monterey Rd., Stuart at the curb in your blue container in Martin County? Or maybe your area is still using the blue and yellow

Florida, University of

264

75 FR 26709 - Clarke County Water Supply Project, Clarke County, IA  

Federal Register 2010, 2011, 2012, 2013

...Conservation Service Clarke County Water Supply Project, Clarke County...of the National Environmental Policy Act of 1969; the Council on...prepared for the Clarke County Water Supply Project, Clarke County...NRCS information related to water supply demand requirements...

2010-05-12

265

75 FR 45557 - Prevailing Rate Systems; Definition of Tulsa County, OK, and Angelina County, TX, to...  

Federal Register 2010, 2011, 2012, 2013

...County, OK, and Angelina County, TX, to Nonappropriated...SUMMARY: The U.S. Office of Personnel...that would define Tulsa County, Oklahoma, as an area...SUPPLEMENTARY INFORMATION: The U.S. Office of Personnel...that would define Tulsa County, Oklahoma, as an...

2010-08-03

266

76 FR 30152 - East Calloway County Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of...  

Federal Register 2010, 2011, 2012, 2013

...FRL-9310-4] East Calloway County Middle School Mercury Spill Site, Murray, Calloway County...the East Calloway County Middle School Mercury Spill Site located in Murray, Calloway...County [[Page 30153

2011-05-24

267

75 FR 43039 - Fresh Prunes Grown in Designated Counties in Washington and in Umatilla County, OR; Suspension of...  

Federal Register 2010, 2011, 2012, 2013

...Prunes Grown in Designated Counties in Washington and in Umatilla County, OR; Suspension...assessment requirements prescribed under the Washington-Oregon fresh prune marketing order...prunes grown in designated counties in Washington and in Umatilla County, Oregon,...

2010-07-23

268

Trouble Brewing in Orange County. Policy Brief  

ERIC Educational Resources Information Center

Orange County will soon face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that Orange County faces a total $41.2 billion liability for retiree benefits that are underfunded--including $9.4 billion for the county pension system and an estimated

Buck, Stuart

2010-01-01

269

Grayson County Counselor-In-Training  

E-print Network

on Thursday, March 3, 2011 at 7:00pm at the Twin County United Way in Galax. Interviews will last- 0 - 2011 Grayson County Counselor-In-Training Application Please return to: Daniel Collins, 4-H Extension Agent Grayson County Extension Office PO Box 129 Independence, VA 24348 276-773-2491 (phone) 276

Liskiewicz, Maciej

270

THE VASCULAR FLORA OF CLINTON COUNTY, OHIO  

Microsoft Academic Search

The present investigation summarizes the existing, available knowledge of the occurrence and distribution of vascular plants growing without cultivation in Clinton County, Ohio. No previous floras of this county have been published. Field collections in Clinton County have been made by the writer from 1939 to the present time during vacations and spare time at varying intervals, the longest period

EDWARD E. TERRELL

271

Tennessee Higher Education County Profiles, 2009  

ERIC Educational Resources Information Center

This report presents a localized perspective on Tennessee higher education for the year 2009, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; and (4) number of county residents participating in the state's primary need-based and merit-based financial aid

Tennessee Higher Education Commission, 2009

2009-01-01

272

Community Types and Mortality in Georgia Counties  

ERIC Educational Resources Information Center

Using an "ecological regional analysis" methodology for defining types of communities and their associated mortality rates, this study of Georgia's 159 counties finds that the suburban and town centered counties have low mortality while the city-centered type predicts low mortality for the whites. The military-centered counties do not predict. The

Young, Frank W.

2012-01-01

273

Tennessee Higher Education County Profiles, 2007  

ERIC Educational Resources Information Center

This report presents a localized perspective on Tennessee higher education for the year 2007, including: (1) county demographic and economic data; (2) information on public and private colleges and universities located in the county; and (4) number of county residents participating in the state's primary need-based and merit-based financial aid

Tennessee Higher Education Commission, 2007

2007-01-01

274

Baker County Cooperative Extension 2013 Annual Report  

E-print Network

and Agricultural Sciences (UF | IFAS) and the Baker County Board of County Commissioners. Our mission is to provide to visit our website at http://baker.ifas.ufl.edu or give us a call at 904-259-3520 Sincerely, Michael A livestock (n=12) raised in excess of $18,500 from local buyers via the Baker County Fair Livestock Sale

Jawitz, James W.

275

Public Health County of Santa Cruz  

E-print Network

Public Health Division County of Santa Cruz HEALTH SERVICES AGENCY POST OFFICE BOX 962, 1080 Date: December 3, 2009 Phone: 831-454-4000 Email: hsadocpio@co.santa-cruz.ca.us County Health Department Announces Flu Vaccine Clinic Santa Cruz County Public Health will hold an additional walk

California at Santa Cruz, University of

276

24 CFR 570.307 - Urban counties.  

Code of Federal Regulations, 2013 CFR

...qualification documentation from counties at such time, and in such...applicable portions of each eligible county for purposes of fund allocation...information available from the U.S. Bureau of the Census with...information provided by the county and its included units of...

2013-04-01

277

24 CFR 570.307 - Urban counties.  

Code of Federal Regulations, 2012 CFR

...qualification documentation from counties at such time, and in such...applicable portions of each eligible county for purposes of fund allocation...information available from the U.S. Bureau of the Census with...information provided by the county and its included units of...

2012-04-01

278

24 CFR 570.307 - Urban counties.  

...qualification documentation from counties at such time, and in such...applicable portions of each eligible county for purposes of fund allocation...information available from the U.S. Bureau of the Census with...information provided by the county and its included units of...

2014-04-01

279

The Licking County Writing Project.  

ERIC Educational Resources Information Center

The 24 articles in this special journal resulted from the Licking County (Ohio) Writing Project. The articles provide information about the following: (1) how to motivate students and teachers, (2) prewriting activities, (3) lightening the teacher's grading load, (4) the uses of conferences, (5) sustained silent reading, (6) traditional techniques

Davis, James E., Ed.; Davis, Hazel K., Ed.

1982-01-01

280

Hill County Secondary Data Analysis  

E-print Network

Statistics (2012) *Chronic Lower Respiratory Disease **Unintentional Injuries: External cause of injury) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. Unintentional Injuries Level1 18.0% 14.0% 13.8% Uninsured Adults (Age Children (Age

Maxwell, Bruce D.

281

Glacier County Secondary Data Analysis  

E-print Network

Statistics (2012) *Chronic Lower Respiratory Disease **Unintentional Injuries: External cause of injury) Leading Causes of Death County1 Montana1,2 Nation2 1. Heart Disease 2. Cancer 3. Unintentional Injuries Level1 27.0% 14.0% 13.8% Uninsured Adults (Age Children (Age

Maxwell, Bruce D.

282

Brandon field, Kiowa County, Colorado  

Microsoft Academic Search

Brandon field, first commercial Mississippian oil production in Colorado, located in Kiowa County in SE. Colorado, was discovered in Aug. 1965. Initial completion was made in carbonate rocks of the Osagean Series (Mississippian) at a depth of 4,770 ft. The 24 producing wells drilled to date have established production also from the Mississippian St. Louis, Spergen, and Warsaw formations, covering

Stoddard

1968-01-01

283

Bedford County Employer Needs Assessment.  

ERIC Educational Resources Information Center

In the summer of 1995, 371 establishments in Bedford County, Pennsylvania, were surveyed regarding their opinions of the quality of the local work force. The purpose of the study was to provide evaluation data for planning and curriculum development at the secondary and post-secondary school levels. Forty-three percent of the employers responded.

Rephann, Terance J.

284

COUNTY COMMISSIONERS Wesley Wainwright, Chairman  

E-print Network

Partnership/Horticulture-Carolyn Saft Family and Consumer Sciences/4-H-Cathy Rogers Regional Specialized Dairy Mellisa Fennell John Burley Darlene Galloway Shannon Croft #12;2011-2012 SUWANNEE COUNTY 4-H COUNCIL Flag-Markham Family Welcome-Andrew Horvath Slide Show Introduction and Acknowledgment of Guests-Council

Jawitz, James W.

285

2013 County Commissioner Satisfaction Survey  

E-print Network

by the CSU Research Integrity and Compliance Review Office in 2012. A five-point scale was used and service level of county Extension personnel; (4) the perceived value to citizens of Extension programs envelope were also enclosed, offering the choice to complete a paper survey. The letter stressed

286

SEM ----| | | | -Powered by Discuz! county: | | | | | | | | | | |  

E-print Network

SEM - - - - | | | | - Powered by Discuz! » county: | | | | | | | | | | | » - » » SEM FAQ chem8ers Are you benning today? > 17 1/2 1 2 >> | | |: [ ] [ ] SEM Reuben Qualified UID 5 2 3943 272 225 Vip 3 2965 100 2006-6-17 Inorganic Chem #12007-7-4 22:00 SEM SEM Amplification and Transcription

Mark, James E.

287

GEOPHAGY IN A MISSISSIPPI COUNTY  

Microsoft Academic Search

Geophagy (earth eating) is practiced by black women and black children of both sexes, but by few or none of the adult black males in Holmes County, Mississippi. Geophagy does not stem from physiological disorder nor appetite aberration. It appears to be a custom brought by slaves from the Old World. Edible clays are obtained from the B soil horizon.

DONALD E. VERMEER; DENNIS A. FRATE

1975-01-01

288

Workforce Solutions for Tarrant County  

E-print Network

locations. ·has received national, state, and local recognition and awards for innovation, quality services,178 homeless individuals were placed in jobs ·12,579 veterans were placed in jobs · 2,182 ex-offenders were Chamber of Commerce Link America United Way of Tarrant County Gaylord Texan Resort Local 776 University

Texas at Arlington, University of

289

Rosebud County Secondary Data Analysis  

E-print Network

65+ 9% 59% 11% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.7% 50 prevalence (Heart Attack) 4.6% 4.1% 6.0% All Sites Cancer 472.3 (Region 1) 455.5 543.2 1 Community River, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per

Maxwell, Bruce D.

290

Yellowstone County Secondary Data Analysis  

E-print Network

65+ 7% 62% 14% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.1% 50 Myocardial Infarction prevalence (Heart Attack) 4.1% 4.1% 6.0% All Sites Cancer 510.8 (Region 3) 455, Yellowstone, Big Horn, and Carbon Chronic Disease Hospitalization Rates County Montana Stroke1 Per

Maxwell, Bruce D.

291

Teton County Secondary Data Analysis  

E-print Network

65+ 5% 60% 18% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.6% 50 prevalence (Heart Attack) 4.4% 4.1% 6.0% All Sites Cancer 461.9 455.5 543.2 1 Community Health Data, MT, Pondera, Teton, Chouteau, and Cascade Chronic Disease Hospitalization Rates County Montana Stroke

Maxwell, Bruce D.

292

Phillips County Secondary Data Analysis  

E-print Network

+ Male Female Male Female Male Female Myocardial Infarction prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1, Fallon, Powder River, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana

Maxwell, Bruce D.

293

Fallon County Secondary Data Analysis  

E-print Network

65+ 7% 60% 20% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.5% 50 prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community Health Data, MT, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100

Maxwell, Bruce D.

294

Lincoln County Secondary Data Analysis  

E-print Network

+ Male Female Male Female Male Female prevalence (Heart Attack) 5.0% 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455.5 543.2 1 Community, Sanders, Lake, Mineral, Missoula, and Ravalli Chronic Disease Hospitalization Rates County

Maxwell, Bruce D.

295

Pondera County Secondary Data Analysis  

E-print Network

65+ 6% 59% 17% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.2% 50 prevalence (Heart Attack) 4.4% 4.1% 6.0% All Sites Cancer 461.9 455.5 543.2 1 Community Health Data, MT, Pondera, Teton, Chouteau, and Cascade Chronic Disease Hospitalization Rates County Montana Stroke

Maxwell, Bruce D.

296

Dawson County Secondary Data Analysis  

E-print Network

65+ 6% 62% 18% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.8% 50 Infarction prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community River, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per

Maxwell, Bruce D.

297

Lake County Secondary Data Analysis  

E-print Network

-64 65+ Male Female Male Female Male Female 49.6% Diabetes prevalence 6.7% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart Attack) 4.5% 4.1% 6.0% All Hospitalization Rates County Montana Stroke Per 100,000 population 1 164.0 182.2 Diabetes Per 100,000 population 1

Maxwell, Bruce D.

298

Blaine County Secondary Data Analysis  

E-print Network

65+ 9% 58% 13% 6% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.4% 50 prevalence 3.3% 2.5% 2.6% Diabetes prevalence 9.4% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart, Pondera, Teton, Chouteau, and Cascade Chronic Disease Hospitalization Rates County Montana Stroke

Maxwell, Bruce D.

299

Hydrogeology of Webb County, Texas  

USGS Publications Warehouse

Introduction: Webb County, in semiarid South Texas on the U.S.-Mexico border, is a region confronted by increasing stresses on natural resources. Laredo (fig. 1), the largest city in Webb County (population 193,000 in 2000), was one of the 10 fastest-growing metropolitan areas in the country during 1990-2000 (Perry and Mackun, 2001). Commercial and industrial activities have expanded throughout the region to support the maquiladora industry (manufacturing plants in Mexico) along the border and other growth as a result of the passage of the North American Free Trade Agreement. The Rio Grande currently (2002) is the primary source of public water supply for Laredo and other cities along the border in Webb County (fig. 1). Other cities, such as Bruni and Mirando City in the southeastern part of the county, rely on ground-water supplies to meet municipal demands. Increased water demand associated with development and population growth in the region has increased the need for the City of Laredo and Webb County to evaluate alternative water sources to meet future demand. Possible options include (1) supplementing the surface-water supply with ground water, and (2) applying artificial storage and recovery (ASR) technology to recharge local aquifers. These options raise issues regarding the hydraulic capability of the aquifers to store economically substantial quantities of water, current or potential uses of the resource, and possible effects on the quality of water resulting from mixing ground water with alternative source waters. To address some of these issues, the U.S. Geological Survey (USGS), in cooperation with the City of Laredo, began a study in 1996 to assess the ground-water resources of Webb County. A hydrogeologic study was conducted to review and analyze available information on the hydrogeologic units (aquifers and confining units) in Webb County, to locate available wells in the region with water-level and water-quality information from the aquifers, and to analyze the hydraulic properties of the aquifers. The purpose of this report is to document the findings of the study. The information is organized by hydrogeologic unit and presented on this and six other sheets.

Lambert, Rebecca B.

2004-01-01

300

Thermal-hydraulic analyses of the TN-24P cask loaded with consolidated and unconsolidated spent nuclear fuel  

SciTech Connect

This paper presents the results of comparisons of COBRA-SFS (spent fuel storage) temperature predictions with experimental data from the TN-24P (Transnuclear) spent fuel storage cask loaded with unconsolidated and consolidated spent PWR fuel. Peak cladding temperature predictions using the COBRA-SFS code are compared with test data and predicted axial and radial temperature distributions are compared with measured temperature profiles. The pre-test accuracy of the COBRA-SFS code in predicting temperature distributions is discussed, along with the effect of post-test model improvements on temperature predictions. This paper also briefly describes the COBRA-SFS code, which is designed to accurately predict flow and temperature distributions in spent nuclear fuel storage and transportation systems. 6 refs., 14 figs.

Michener, T.E.; McKinnon, M.A.; Rector, D.R.; Creer, J.M.

1989-06-01

301

Isolation and characterization of Tn5 insertion mutants of Pseudomonas syringae pv. syringae altered in the production of the peptide phytotoxin syringotoxin.  

PubMed Central

A syringotoxin-producing strain of Pseudomonas syringae pv. syringae (B457) was subjected to Tn5 mutagenesis by the transposon vector pSUP1011. Analyses of auxotrophs obtained suggested simple random insertions of Tn5. Syringotoxin-negative mutants arose at a frequency of about 0.28%. In a Southern blot analysis, the loss of toxin production was associated with Tn5 insertions into chromosomal EcoRI fragments of about 10.5, 17.8, and 19.3 kilobases. Data from a Southern blot analysis of SstI-digested DNA from these mutants suggest that the 10.5- and 17.8-kilobase EcoRI fragments may be adjacent to or near each other. Mutants that produced only 3 to 4% wild-type toxin levels also were identified. Images PMID:2995307

Morgan, M K; Chatterjee, A K

1985-01-01

302

Tiu Ch Chn La D n Khi Phc Sm Vo ngy 21 thng 4 nm 2011, cc y Vin nh Gi Tn Hi  

E-print Network

Tiêu Chí Chn La D Án Khôi Phc Sm Vào ngày 21 tháng 4 nm 2011, các y Viên ánh Giá Tn Hi Tài Nguyên các y viên trong vic tr li nh ban u các tài nguyên thiên nhiên ã b tn hi và/hoc n bù các thit hi tm s tn hi cng là mt yu t quan trng trong quy trình la chn d án); Kh nng thành công ca tng gii pháp thay

303

Pressure Dependence of the Electrical Resistivity and TN of the Ternary Uranium Antiferromagnets UNiSn, UPdIn and UPdSn  

NASA Astrophysics Data System (ADS)

The effects of pressure on the resistivity ? and the Nel temperature TN of UNiSn, UPdIn and UPdSn have been investigated up to 25 kbar. With the applied pressure, semiconducting behavior in UNiSn is suppressed and the presence of the Kondo like effect is revealed in UPdSn. Positive dTN/dP of the ternary UTX is compared with that of binary U compounds and discussed in terms of the interatomic spacing of U atoms.

Kurisu, Makio; Kawanaka, Hirofumi; Takabatake, Toshiro; Fujii, Hironobu

1991-11-01

304

Chester County ground-water atlas, Chester County, Pennsylvania  

USGS Publications Warehouse

Chester County encompasses 760 square miles in southeastern Pennsylvania. Groundwater- quality studies have been conducted in the county over several decades to address specific hydrologic issues. This report compiles and describes water-quality data collected during studies conducted mostly after 1990 and summarizes the data in a county-wide perspective. In this report, water-quality constituents are described in regard to what they are, why the constituents are important, and where constituent concentrations vary relative to geology or land use. Water-quality constituents are grouped into logical units to aid presentation: water-quality constituents measured in the field (pH, alkalinity, specific conductance, and dissolved oxygen), common ions, metals, radionuclides, bacteria, nutrients, pesticides, and volatile organic compounds.Waterquality constituents measured in the field, common ions (except chloride), metals, and radionuclides are discussed relative to geology. Bacteria, nutrients, pesticides, and volatile organic compounds are discussed relative to land use. If the U.S. Environmental Protection Agency (USEPA) or Chester County Health Department has drinkingwater standards for a constituent, the standards are included. Tables and maps are included to assist Chester County residents in understanding the water-quality constituents and their distribution in the county. Ground water in Chester County generally is of good quality and is mostly acidic except in the carbonate rocks and serpentinite, where it is neutral to strongly basic. Calcium carbonate and magnesium carbonate are major constituents of these rocks. Both compounds have high solubility, and, as such, both are major contributors to elevated pH, alkalinity, specific conductance, and the common ions. Elevated pH and alkalinity in carbonate rocks and serpentinite can indicate a potential for scaling in water heaters and household plumbing. Low pH and low alkalinity in the schist, quartzite, and gneiss rocks can indicate a potential for corrosive water. The only constituent measured in the field that has a USEPA Secondary Maximum Contaminant Level (SMCL) is pH. The SMCL for pH is 6.5-8.5; 64 percent of samples analyzed for pH were acidic (below pH 6.5). Only 1 percent of samples were basic (above pH 8.5). Of the common ions, the USEPA has SMCLs for chloride, sulfate, and total dissolved solids. The USEPA has a SMCL and a Primary Maximum Contaminant Level (PMCL) for fluoride. Chloride is more closely related to land use than geology. In Chester County, chloride exceeded the SMCL (250 mg/L) only in 5 percent of the services (commercial services, community services, and military) land-use areas. No samples analyzed for sulfate exceeded the SMCL (250 mg/L). Only 3 percent of samples analyzed for total dissolved solids exceeded the SMCL (500 milligrams per liter) (mg/L). No samples analyzed for fluoride equaled or exceeded the SMCL (2.0 mg/L) or PMCL (4.0 mg/L). Iron concentrations exceeded the USEPA SMCL in 11 percent of samples and were highest in schist (14 percent) and gneiss (13 percent). Manganese concentrations exceeded the SMCL in 19 percent of samples and were highest in quartzite and schist (both 28 percent). Lead and arsenic were present in low concentrations: the highest concentrations of lead occurred in water from quartzite (8 percent exceeded the USEPA Action Level), and arsenic was detected mostly in Triassic sedimentary rocks (9 percent exceeded the USEPA PMCL). The highest concentrations of copper occurred more frequently in quartzite rocks, and to a lesser extent were evenly distributed between ground water in gneiss, schist, and Triassic sedimentary rocks. Elevated concentrations of radon-222 and the combined radium-226/radium-228 radionuclides were common in water from quartzite and schist. Gross alpha and gross beta particle activities were elevated in water from quartzite and carbonate rocks. In contrast, elevated concentrations of uranium prima

Ludlow, Russell A.; Loper, Connie A.

2004-01-01

305

Monroe County Library System: Pathfinders  

NSDL National Science Digital Library

Named after President James Monroe, Monroe County is part of upstate New York. The industrial metropolis of Rochester sits within its boundaries, and one of its key institutions is the Monroe County Library System. Over the past several years, the System has created this treasure trove of digital collections which dissect and organize the history of the area through historic maps, architectural guides, and an online history tour of places in Rochester that played a role in the abolition movement and the Civil War. First-time visitors might want to start out by looking over the "Raising the Flag: Patriotism in Rochester, 1892-1922" photo gallery and then move on the Susan B. Anthony Letters Collection. Library staff members have also created nine fine research guides for scholars and others, and they include a guide to Rochester immigrants and a house history guide.

306

Missoula County Secondary Data Analysis  

E-print Network

Female Male Female Male Female 50.4% 49.6% 50.1% 49.9% 49.2% 50.8% Race/Ethnic Distribution Myocardial Infarction prevalence (Heart Attack) 3.5% 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455 Rates County Montana Stroke1 Per 100,000 population 173.0 182.2 Diabetes1 Per 100,000 population

Maxwell, Bruce D.

307

Richland County Secondary Data Analysis  

E-print Network

% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.8% 50.2% 50.1% 49.9% 49.2% 50 prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community Health Data, MT, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100

Maxwell, Bruce D.

308

Gallatin County Secondary Data Analysis  

E-print Network

Male Female Male Female Male Female 52.0% 48.0% 50.1% 49.9% 49.2% 50.8% Race prevalence (Heart Attack) 2.4% 4.1% 6.0% All Sites Cancer 416.6 (Region 4) 455.5 543.2 1 Community Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 152.4 182

Maxwell, Bruce D.

309

Granite County Secondary Data Analysis  

E-print Network

% 7% 62% 13% Gender1 Male Female Male Female Male Female 50.8% 49.2% 50.1% 49.9% 49.2% 50 Infarction prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 143.4 (Region 4

Maxwell, Bruce D.

310

Powell County Secondary Data Analysis  

E-print Network

Male Female Male Female Male Female 61.2% 38.8% 50.1% 49.9% 49.2% 50.8% Race Infarction prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 190.2 182

Maxwell, Bruce D.

311

Musselshell County Secondary Data Analysis  

E-print Network

% 63% 14% 7% 62% 13% Gender1 Male Female Male Female Male Female 48.6% 51.4% 50.1% 49.9% 49 Myocardial Infarction prevalence (Heart Attack) 4.3% 4.1% 6.0% All Sites Cancer 510.8 455.5 543.2 1 Horn, and Carbon Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100

Maxwell, Bruce D.

312

Meagher County Secondary Data Analysis  

E-print Network

% 62% 13% Gender1 Male Female Male Female Male Female 50.2% 49.8% 50.1% 49.9% 49.2% 50.8% Race prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 143.4 (Region 4) 182

Maxwell, Bruce D.

313

Madison County Secondary Data Analysis  

E-print Network

% 62% 13% Gender1 Male Female Male Female Male Female 51.0% 49.0% 50.1% 49.9% 49.2% 50.8% Race prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 177.5 182.2 Diabetes1

Maxwell, Bruce D.

314

Liberty County Secondary Data Analysis  

E-print Network

Female Male Female Male Female 49.2% 50.8% 50.1% 49.9% 49.2% 50.8% Race/Ethnic Distribution Infarction prevalence (Heart Attack) 4.4% 4.1% 6.0% All Sites Cancer 461.9 455.5 543.2 1 Community Rates County Montana Stroke1 Per 100,000 population 180.5 182.2 Diabetes1 Per 100,000 population

Maxwell, Bruce D.

315

Custer County Secondary Data Analysis  

E-print Network

% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.1% 50.9% 50.1% 49.9% 49.2% 50 prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community Health Data, MT, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100

Maxwell, Bruce D.

316

Cascade County Secondary Data Analysis  

E-print Network

Female Male Female Male Female 49.6% 50.4% 50.1% 49.9% 49.2% 50.8% Race/Ethnic Distribution prevalence (Heart Attack) 4.0% 4.1% 6.0% All Sites Cancer 461.9 (Region 2) 455.5 543.2 1 Community Rates County Montana Stroke1 Per 100,000 population 219.1 182.2 Diabetes1 Per 100

Maxwell, Bruce D.

317

Beaverhead County Secondary Data Analysis  

E-print Network

Male Female Male Female Male Female 51.3% 48.7% 50.1% 49.9% 49.2% 50.8% Race prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 106.2 182

Maxwell, Bruce D.

318

Flathead County Secondary Data Analysis  

E-print Network

Female Male Female Male Female 50.0% 50.0% 50.1% 49.9% 49.2% 50.8% Race/Ethnic Distribution Myocardial Infarction prevalence (Heart Attack) 3.7% 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455 Rates County Montana Stroke1 Per 100,000 population 194.2 182.2 Diabetes1 Per 100,000 population

Maxwell, Bruce D.

319

Fergus County Secondary Data Analysis  

E-print Network

% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.1% 50.9% 50.1% 49.9% 49.2% 50 Infarction prevalence (Heart Attack) 4.3% 4.1% 6.0% All Sites Cancer 510.8 455.5 543.2 1 Community, and Carbon Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population

Maxwell, Bruce D.

320

Wheatland County Secondary Data Analysis  

E-print Network

% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.7% 50.3% 50.1% 49.9% 49.2% 50 prevalence (Heart Attack) 4.3% 4.1% 6.0% All Sites Cancer 510.8 455.5 543.2 1 Community Health Data, MT Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 173.8 182

Maxwell, Bruce D.

321

Valley County Secondary Data Analysis  

E-print Network

% 7% 62% 13% Gender1 Male Female Male Female Male Female 49.3% 50.7% 50.1% 49.9% 49.2% 50 Infarction prevalence (Heart Attack) 5.5% 4.1% 6.0% All Sites Cancer 472.3 455.5 543.2 1 Community River, Carter, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100

Maxwell, Bruce D.

322

Jefferson County Secondary Data Analysis  

E-print Network

Male Female Male Female Male Female 50.2% 49.8% 50.1% 49.9% 49.2% 50.8% Race prevalence (Heart Attack) 3.4% 4.1% 6.0% All Sites Cancer 416.6 455.5 543.2 1 Community Health Data, MT Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 123.7 182.2 Diabetes1

Maxwell, Bruce D.

323

Mineral County Secondary Data Analysis  

E-print Network

% 7% 62% 13% Gender1 Male Female Male Female Male Female 50.8% 49.2% 50.1% 49.9% 49.2% 50 prevalence (Heart Attack) 4.0% 4.1% 6.0% All Sites Cancer 466.5 455.5 543.2 1 Community Health Data, MT, Missoula, and Ravalli Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100

Maxwell, Bruce D.

324

Roosevelt County Secondary Data Analysis  

E-print Network

Female Male Female Male Female 49.4% 50.6% 50.1% 49.9% 49.2% 50.8% Race/Ethnic Distribution White 1 37 prevalence 3.2% 2.5% 2.6% Diabetes prevalence 12.9% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart, and Treasure Chronic Disease Hospitalization Rates County Montana Stroke Per 100,000 population 1 196.5 182

Maxwell, Bruce D.

325

Broadwater County Secondary Data Analysis  

E-print Network

% 7% 62% 13% Gender 1 Male Female Male Female Male Female 51.1% 48.9% 50.1% 49.9% 49.2% 50.8% Race prevalence 1.9% 2.5% 2.6% Diabetes prevalence 5.1% 6.2% 8.3% Acute Myocardial Infarction prevalence (Heart, Meagher, Silver Bow, Gallatin, Park, Madison, and Beaverhead Chronic Disease Hospitalization Rates County

Maxwell, Bruce D.

326

Carbon County Secondary Data Analysis  

E-print Network

Male Female Male Female Male Female 50.6% 49.4% 50.1% 49.9% 49.2% 50.8% Race prevalence (Heart Attack) 4.3% 4.1% 6.0% All Sites Cancer 510.8 455.5 543.2 1 Community Health Data, MT Chronic Disease Hospitalization Rates County Montana Stroke1 Per 100,000 population 183.6 182

Maxwell, Bruce D.

327

Sanders County Secondary Data Analysis  

E-print Network

Female Male Female Male Female 49.9% 50.1% 50.1% 49.9% 49.2% 50.8% Race/Ethnic Distribution Infarction prevalence (Heart Attack) 4.0% (Region 5) 4.1% 6.0% All Sites Cancer 466.5 (Region 5) 455 Rates County Montana Stroke1 Per 100,000 population 138.0 182.2 Diabetes1 Per 100,000 population

Maxwell, Bruce D.

328

Proceedings of the 1995 ACM/SIGAPP Symposium on Applied Computing, February 26-28, 1995, Nashville, TN, pp. 351-356, ACM Press.  

E-print Network

problem. In Section 3 the fundamentals of genetic algorithms are reviewed. In Section 4 a genetic, TN, pp. 351-356, ACM Press. Detecting Multiple Outliers in Regression Data Using Genetic Algorithms{ Genetic Algorithm, Outlier, Least Squares, Re- gression Abstract In this paper a new technique

Wainwright, Roger L.

329

Calcium release triggered by activation of the acetycholine receptor in M1-CNiFERs brings the yellow and blue "arms" of TN-XXL closer  

E-print Network

the yellow and blue "arms" of TN-XXL closer together, changing its color. In control CNiFERS, which lack Publishers Ltd: Nature Neuroscience, Volume 13, 127-132, 2010.) Home Research Funding Training Health & Education e-Advances Stories of Discovery Science Snippets Health Information Science Education Resource

Kleinfeld, David

330

Mentee Registration Box 70592 ~ Johnson City, TN 37614 ~ Office 423/439-5602 ~ Fax 423-439-4840 ~ mpamentoring@etsu.edu  

E-print Network

Mentee Registration Box 70592 ~ Johnson City, TN 37614 ~ Office 423/439-5602 ~ Fax 423 Advisement (MPA) Office on the 2nd floor of the Culp Center inside the ARC. With this form, you of Study: Chiropractic (PCHI) Dentistry (PDNT) Medicine (PMED) Optometry (POPT) Osteopathic Medicine (POPM

Karsai, Istvan

331

Plasmid heterogeneity and identification of a Tn5281-like element in clinical isolates of high-level gentamicin-resistant Enterococcus faecium isolated in the UK.  

PubMed

Ten clinical isolates of high-level gentamicin-resistant (HLGR) Enterococcus faecium, collected from six hospitals throughout the UK, were studied to determine whether HLGR was attributed to widespread dissemination of a single plasmid or whether different plasmid types were implicated in the dissemination of this phenotype. HLGR was attributed to the presence of the AAC6'-APH2" bifunctional aminoglycoside modifying enzyme. The aac6'-aph2" gene was present on a 70 kb plasmid in all ten isolates. Conjugation studies indicated that the HLGR marker could transfer with varying frequency, with or without the associated 70 kb plasmid. Detailed molecular genetic analysis suggested that four of the isolates harboured a transposon similar to Tn5281, originally identified in Enterococcus faecalis strain HH22 isolated in the USA. The UK transposon, however, lacked the two symmetrically located HaeIII sites found in Tn5281. The six remaining isolates appeared to have a Tn5281-truncated structure in which the aac6'-aph2" gene is flanked by an IS256 element at the 5' end. Further studies with nine restriction endonucleases showed that the aac6'-aph2" gene was associated with two different plasmid types in E. faecium. Pulsed-field gel electrophoresis (PFGE) analysis identified three different patterns. The four E. faecium isolates harbouring the Tn5281-like structure were indistinguishable from each other, while the remaining six isolates exhibited two distinct PFGE patterns. This is the first study to indicate that there is heterogeneity among the plasmids that confer the HLGR phenotype in E. faecium isolates in the UK and to report on the presence of a transposon, similar to Tn5281, in E. faecium harbouring the aac6'-aph2" gene. PMID:10382883

Simjee, S; Fraise, A P; Gill, M J

1999-05-01

332

Transposon Tn7 Preferentially Inserts into GAAoTTC Triplet Repeats under Conditions Conducive to YoRoY Triplex Formation  

PubMed Central

Background Expansion of an unstable GAATTC repeat in the first intron of the FXN gene causes Friedreich ataxia by reducing frataxin expression. Structure formation by the repeat has been implicated in both frataxin repression and GAATTC instability. The GAATTC sequence is capable of adopting multiple non-B DNA structures including YRY and RRY triplexes. Lower pH promotes the formation of YRY triplexes by GAATTC. Here we used the bacterial transposon Tn7 as an in vitro tool to probe whether GAATTC repeats can attract a well-characterized recombinase. Methodology/Principal Findings Tn7 showed a pH-dependent preference for insertion into uninterrupted regions of a Friedreich ataxia patient-derived repeat, inserting 48, 39 and 14 percent of the time at pH 7, pH 8 and pH 9, respectively. Moreover, Tn7 also showed orientation and region specific insertion within the repeat at pH 7 and pH 8, but not at pH 9. In contrast, transposon Tn5 showed no strong preference for or against the repeat during in vitro transposition at any pH tested. YRY triplex formation was reduced in predictable ways by transposon interruption of the GAATTC repeat. However, transposon interruptions in the GAATTC repeats did not increase the in vitro transcription efficiency of the templates. Conclusions/Significance We have demonstrated that transposon Tn7 will recognize structures that form spontaneously in GAATTC repeats and insert in a specific orientation within the repeat. The conditions used for in vitro transposition span the physiologically relevant range suggesting that long GAATTC repeats can form triplex structures in vivo, attracting enzymes involved in DNA repair, recombination and chromatin modification. PMID:20559546

Mancuso, Miriam; Sammarco, Mimi C.; Grabczyk, Ed

2010-01-01

333

Tn5253 family integrative and conjugative elements carrying mef(I) and catQ determinants in Streptococcus pneumoniae and Streptococcus pyogenes.  

PubMed

The linkage between the macrolide efflux gene mef(I) and the chloramphenicol inactivation gene catQ was first described in Streptococcus pneumoniae (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in Streptococcus pyogenes (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253 family integrative and conjugative elements (ICEs). The ICE from S. pneumoniae (ICESpn529IQ) was sequenced, whereas the ICEs from S. pyogenes (ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share the intSp 23FST81 integrase gene and an identical Tn916 fragment, whereas ICESpy005IQ has int5252 and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (intSp 23FST81 or int5252). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation, mef(I) and catQ were cotransferred at a high frequency from S. pyogenes Spy005 and at very low frequencies from S. pneumoniae Spn529 and S. pyogenes Spy029. PMID:25070090

Mingoia, Marina; Morici, Eleonora; Morroni, Gianluca; Giovanetti, Eleonora; Del Grosso, Maria; Pantosti, Annalisa; Varaldo, Pietro E

2014-10-01

334

Spatial variability assessment of soil nutrients in an intense agricultural area, a case study of Rugao County in Yangtze River Delta Region, China  

NASA Astrophysics Data System (ADS)

Topsoil samples (0-20 cm) ( n = 237) were collected from Rugao County, China. Geostatistical variogram analysis, sequential Gaussian simulation (SGS), and principal component (PC) analysis were applied to assess spatial variability of soil nutrients, identify the possible areas of nutrient deficiency, and explore spatial scale of variability of soil nutrients in the county. High variability of soil nutrient such as soil organic matter (SOM), total nitrogen (TN), available P, K, Fe, Mn, Cu, Zn, and B concentrations were observed. Soil nutrient properties displayed significant differences in their spatial structures, with available Cu having strong spatial dependence, SOM and available P having weak spatial dependence, and other nutrient properties having moderate spatial dependence. The soil nutrient deficiency, defined here as measured nutrient concentrations which do not meet the advisory threshold values specific to the county for dominant crops, namely rice, wheat, and rape seeds, was observed in available K and Zn, and the deficient areas covered 38 and 11%, respectively. The first three PCs of the nine soil nutrient properties explained 62.40% of the total variance. TN and SOM with higher loadings on PC1 are closely related to soil texture derived from different parent materials. The PC2 combined intermediate response variables such as available Zn and P that are likely to be controlled by land use and soil pH. Available B has the highest loading on PC3 and its variability of concentrations may be primarily ascribed to localized anthropogenic influence. The amelioration of soil physical properties (i.e. soil texture) and soil pH may improve the availability of soil nutrients and the sustainability of the agricultural system of Rugao County.

Zhao, Yongcun; Xu, Xianghua; Darilek, Jeremy Landon; Huang, Biao; Sun, Weixia; Shi, Xuezheng

2009-05-01

335

Digital computer processing of LANDSAT data for North Alabama. [Linestone County, Madison County, Jackson County, Marshall County, and DeKalb County  

NASA Technical Reports Server (NTRS)

Computer processing procedures and programs applied to Multispectral Scanner data from LANDSAT are described. The output product produced is a level 1 land use map in conformance with a Universal Transverse Mercator projection. The region studied was a five-county area in north Alabama.

Bond, A. D.; Atkinson, R. J.; Lybanon, M.; Ramapriyan, H. K.

1977-01-01

336

Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.  

PubMed

Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities. PMID:23737995

Ruiz, Lorena; Motherway, Mary O'Connell; Lanigan, Noreen; van Sinderen, Douwe

2013-01-01

337

Tn5AraOut Mutagenesis for the Identification of Yersinia pestis Genes Involved in Resistance towards Cationic Antimicrobial Peptides  

PubMed Central

Bacterial pathogens display a variety of protection mechanisms against the inhibitory and lethal effects of host cationic antimicrobial peptides (CAMPs). To identify Yersinia pestis genes involved in CAMP-resistance, libraries of DSY101 (KIM6 caf1 pla psa) minitransposon Tn5AraOut mutants were selected at 37C for resistance to the model CAMPs polymyxin B or protamine. This approach targeted genes that needed to be repressed (null mutations) or induced (upstream PBAD insertions) for the detection of CAMP resistance, and predictably for improved pathogen fitness in mammalian hosts. Ten mutants demonstrated increased resistance to polymyxin B or protamine, with the mapped mutations pointing towards genes suspected to participate in modifying membrane components, genes encoding transport proteins or enzymes, or the regulator of a ferrous iron uptake system (feoC). Not all the mutants were resistant to both CAMPs used for selection. None of the polymyxin B- and only some protamine-resistant mutants, including the feoC mutant, showed increased resistance to rat bonchoalveolar lavage fluid (rBALF) known to contain cathelicidin and ?-defensin 1. Thus, findings on bacterial resistance to polymyxin B or protamine dont always apply to CAMPs of the mammalian innate immune system, such as the ones in rBALF. PMID:21575704

Guo, Jitao; Nair, Manoj K. M.; Galvan, Estela M.; Liu, Shu-Lin; Schifferli, Dieter M.

2011-01-01

338

High-Level Gentamicin Resistance Mediated by a Tn4001-Like Transposon in Seven Nonclonal Hospital Isolates of Streptococcus pasteurianus?  

PubMed Central

We report on the first occurrence of high-level gentamicin resistance (MICs ? 512 ?g/ml) in seven clinical isolates of Streptococcus pasteurianus from Hong Kong. These seven isolates were confirmed to be the species S. pasteurianus on the basis of nucleotide sequencing of the superoxide dismutase (sodA) gene. Epidemiological data as well as the results of pulse-field gel electrophoresis analysis suggested that the seven S. pasteurianus isolates did not belong to the same clone. Molecular characterization showed that they carried a chromosomal, transposon-borne resistance gene [aac(6?)Ie-aph(2?)Ia] which was known to encode a bifunctional aminoglycoside-modifying enzyme. The genetic arrangement of this transposon was similar to that of Tn4001, a transposon previously recovered from Staphylococcus aureus and other gram-positive isolates. Genetic linkage with other resistance elements, such as the ermB gene for erythromycin resistance, was not evident. On the basis of these findings, we suggest that routine screening for high-level gentamicin resistance should be recommended for all clinically significant blood culture isolates. This is to avoid the inadvertent use of short-course combination therapy with penicillin and gentamicin, which may lead to the failure of treatment for endocarditis, the selection of drug-resistant Streptococcus pasteurianus and other gram-positive organisms, as well as the unnecessary usage of gentamicin, a drug with potential toxicity. PMID:17371822

Chow, Viola C. Y.; Hawkey, Peter M.; Chan, Edward W. C.; Chin, Miu L.; Au, T. K.; Fung, Danny K. C.; Chan, Raphael C. Y.

2007-01-01

339

The Tn antigen-specific lectin from ground ivy is an insecticidal protein with an unusual physiology.  

PubMed

Leaves of ground ivy (Glechoma hederacea) contain a lectin (called Gleheda) that is structurally and evolutionary related to the classical legume lectins. Screening of a population of wild plants revealed that Gleheda accounts for more than one-third of the total leaf protein in some clones, whereas it cannot be detected in other clones growing in the same environment. Gleheda is predominantly expressed in the leaves where it accumulates during early leaf maturation. The lectin is not uniformly distributed over the leaves but exhibits a unique localization pattern characterized by an almost exclusive confinement to a single layer of palisade parenchyma cells. Insect feeding trials demonstrated that Gleheda is a potent insecticidal protein for larvae of the Colorado potato beetle (Leptinotarsa decemlineata). Because Gleheda is not cytotoxic, it is suggested that the insecticidal activity is linked to the carbohydrate-binding specificity of the lectin, which as could be demonstrated by agglutination assays with different types of polyagglutinable human erythrocytes is specifically directed against the Tn antigen structure (N-acetylgalactosamine O-linked to serine or threonine residues of proteins). PMID:12857814

Wang, Weifang; Hause, Bettina; Peumans, Willy J; Smagghe, Guy; Mackie, Anne; Fraser, Robin; van Damme, Els J M

2003-07-01

340

A second RNA-polymerase can bind specifically to the bla promoter of Tn3, repressing transcription initiation.  

PubMed Central

We showed earlier that the region of the bla promoter of Tn3 protected by the RNA-polymerase (RNAP), has the normal size (about 60bp) at RNAP/promoter molar ratio r less than or equal to 2, but rises to about twice this extent as r increases. We confirm here that the species corresponding to normal and extended footprint distinguish by their electrophoretic mobilities. Furthermore, inspection of the complexes by electron microscopy confirms that at r greater than 2, the bla promoter can bind specifically a second RNAP particle, as compared to the 1:1 complex observed at r less than or equal to 2. At r greater than 2, the ability of the bla promoter to initiate transcription in vitro is repressed when compared to the complex 1:1 obtained at r less than or equal to 2. The unexpected decrease in initiation efficiency as the concentration of RNAP particles is increased, together with the striking sequence homology of the bla promoter with promoters of stable RNA, suggest that in vivo, this promoter could be regulated by growth rate. Images PMID:2838813

Duval-Valentin, G; Schmitt, B; Ehrlich, R

1988-01-01

341

Exploring the molecular basis for selective binding of homoserine dehydrogenase from Mycobacterium leprae TN toward inhibitors: a virtual screening study.  

PubMed

Homoserine dehydrogenase (HSD) from Mycobacterium leprae TN is an antifungal target for antifungal properties including efficacy against the human pathogen. The 3D structure of HSD has been firmly established by homology modeling methods. Using the template, homoserine dehydrogenase from Thiobacillus denitrificans (PDB Id 3MTJ), a sequence identity of 40% was found and molecular dynamics simulation was used to optimize a reliable structure. The substrate and co-factor-binding regions in HSD were identified. In order to determine the important residues of the substrate (L-aspartate semialdehyde (L-ASA)) binding, the ASA was docked to the protein; Thr163, Asp198, and Glu192 may be important because they form a hydrogen bond with HSD through AutoDock 4.2 software. neuraminidaseAfter use of a virtual screening technique of HSD, the four top-scoring docking hits all seemed to cation-? ion pair with the key recognition residue Lys107, and Lys207. These ligands therefore seemed to be new chemotypes for HSD. Our results may be helpful for further experimental investigations. PMID:24469317

Zhan, Dongling; Wang, Dongmei; Min, Weihong; Han, Weiwei

2014-01-01

342

Transposon Tn917lacZ mutagenesis of Bacillus subtilis: identification of two new loci required for motility and chemotaxis.  

PubMed Central

We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot locus. The other locus is closely linked to the first and encodes proteins specifying chemotaxis functions. This locus is designated the cheX locus. Both the mot and cheX loci map close to ptsI. An additional transposon insert that maps in the hag locus was obtained. The pattern of beta-galactosidase expression from some of the transposons suggested that the mot locus is regulated by sigD, a minor sigma factor of B. subtilis. The cheX locus appeared to be under the control of vegetative sigA. Four transposon inserts were mapped to a previously characterized che locus near spcB. These mutants did not produce flagellin and were defective in the methylation of the methyl-accepting chemotaxis proteins. This locus probably encodes proteins required for flagellum biosynthesis and other proteins that are required for the methylation response. Images PMID:2174860

Zuberi, A R; Ying, C W; Parker, H M; Ordal, G W

1990-01-01

343

Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae  

PubMed Central

Type VI protein secretion system (T6SS) is important for bacterial competition through contact-dependent killing of competitors. T6SS delivers effectors to neighboring cells and corresponding antagonistic proteins confer immunity against effectors that are delivered by sister cells. Although T6SS has been found in more than 100 gram-negative bacteria including many important human pathogens, few T6SS-dependent effector and immunity proteins have been experimentally determined. Here we report a high-throughput approach using transposon mutagenesis and deep sequencing (Tn-seq) to identify T6SS immunity proteins in Vibrio cholerae. Saturating transposon mutagenesis was performed in wild type and a T6SS null mutant. Genes encoding immunity proteins were predicted to be essential in the wild type but dispensable in the T6SS mutant. By comparing the relative abundance of each transposon mutant in the mutant library using deep sequencing, we identified three immunity proteins that render protection against killing by T6SS predatory cells. We also identified their three cognate T6SS-secreted effectors and show these are important for not only antibacterial and antieukaryotic activities but also assembly of T6SS apparatus. The lipase and muramidase T6SS effectors identified in this study underscore the diversity of T6SS-secreted substrates and the distinctly different mechanisms that target these for secretion by the dynamic T6SS organelle. PMID:23362380

Dong, Tao G.; Ho, Brian T.; Yoder-Himes, Deborah R.; Mekalanos, John J.

2013-01-01

344

In vitro Tn7 mutagenesis of Haemophilus influenzae Rd and characterization of the role of atpA in transformation.  

PubMed Central

Haemophilus influenzae Rd is a gram-negative bacterium capable of natural DNA transformation. The competent state occurs naturally in late exponential growth or can be induced by a nutritional downshift or by transient anaerobiosis. The genes cya, crp, topA, and sxy (tfoX) are known to function in the regulation of competence development. The phosphoenolpyruvate:carbohydrate phosphotransferase system functions to maintain levels of cyclic AMP necessary for competence development but is not directly involved in regulation. The exact signal(s) for competence and the genes that mediate the signal(s) are still unknown. In an effort to find additional regulatory genes, H. influenzae Rd was mutated by using an in vitro Tn7 system and screened for mutants with a reduced ability to induce the competence-regulatory gene, comA. Insertions in atpA, a gene coding for the alpha subunit of the F1 cytoplasmic domain of the ATP synthase, reduce transformation frequencies about 20-fold and cause a significant reduction in expression of competence-regulatory genes, while the expression of constitutive competence genes is only minimally affected. In addition, we found that an insertion in atpB, which encodes the a subunit of the F0 membrane-spanning domain, has a similar effect on transformation frequencies. PMID:9393695

Gwinn, M L; Stellwagen, A E; Craig, N L; Tomb, J F; Smith, H O

1997-01-01

345

Hudson County Community College Periodic Review Report. Executive Summary.  

ERIC Educational Resources Information Center

Hudson County Community College (HCCC) serves Hudson County, New Jersey. Although the county is the smallest in the state, its 610,000 residents make up one of the most diverse counties in New Jersey. Approximately 40% of residents are Hispanic, 12% are African-American, 10% are Asian, and 35% are White. The county is also home to a growing Middle

Hudson County Community Coll., Jersey City, NJ.

346

32. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

32. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PROFILE OF HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY IN JOHNSON COUNTY, IOWA, 1897. (RIGHT 1/4 OF DRAWING) PLAN OF APPROACH FOR SUTLIFF'S FERRY BRIDGE OVER CEDAR RIVER, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

347

A record twenty-two schools competed in the MAA SE-09 Math Jeopardy Contest on March 13-14, 2009 at Belmont University in Nashville, TN. Teams that participated in  

E-print Network

, TN), and Lenoir Rhyne University (Hickory, NC). Markus Iturriaga Woelfel and Michael W. Berry from: Davidson College (Davidson, NC), two teams from Georgia Southern (Statesboro, GA), University of Tennessee-Martin (Martin, TN), Berry College, (Mount Berry, GA), Bennett College (Greensboro, NC), Furman University

Berry, Michael W.

348

About Mobile County Public School System Mobile County Public School System (MCPSS) has an enrollment of  

E-print Network

residents work in education, healthcare, finance, manufacturing or transportation. The Mobile County Public Public education Size $775 million annual budget Case Study Mobile County Public Schools Drive to Help. Peek is a third-generation Mobile County educator who worked her way up through the school system

Fisher, Kathleen

349

The Economic Impact of Schenectady County Community College on Schenectady County, 1981-82.  

ERIC Educational Resources Information Center

This report on the economic impact of Schenectady County Community College (SCCC) uses a modification of the Caffrey and Isaacs model to assess SCCC-related local business volume, SCCC costs and benefits to the Schenectady County government, and the likely impact on the county if SCCC did not exist. Part I provides background to the study,

Chestnut, Erma Ruth

350

Martin County Master Gardeners In cooperation with the Martin County Library System  

E-print Network

Martin County Master Gardeners In cooperation with the Martin County Library System Present Plants of charge and sponsored by University of Florida/IFAS/Martin County Master Gardeners. Holiday plants will be available for purchase. Light refreshments will be served. http://martin.ifas.ufl.edu phone: 772

Watson, Craig A.

351

75 FR 31463 - Comal County Regional Habitat Conservation Plan, Comal County, TX  

Federal Register 2010, 2011, 2012, 2013

...SUMMARY: Comal County, Texas (Applicant), has applied to the U.S. Fish and Wildlife...Policy Act (NEPA; 42 U.S.C. 4371 et seq...of an ITP to Comal County; and that the Applicant...going to the Comal County Web site at http...Field Supervisor, by U.S. mail at...

2010-06-03

352

Landsliding in Allegheny County, Pennsylvania  

USGS Publications Warehouse

Man should proceed with caution if modifications such as loading, excavation, or changes of the water regime are contemplated for slopes in Allegheny County, especially those slopes described on the map as highly sensitive to disturbance by man. Features indicative of unstable slope conditions include: cracks in buildings, yard walls, and pools; doors and windows that jam; fences and other linear features bowed out of line; tilted trees and utility poles; cracks and steplike ground features; hummocky ground; and water seeps. Geologic factors related to the landsliding process include rock types, layering, fracturing, and attitude; nature of soil cover; permeability of rocks and soils; and steepness of slope.

Briggs, Reginald Peter; Pomeroy, John S.; Davies, William E.

1975-01-01

353

ODYSSEY ENABLED TN.#: 778871  

E-print Network

to be responsible for a highly undesirable property, carcinogenicity. Initially, asbestos was the name of a mineral in cosmetics, dietary food supplements, and cer- tain drugs (Walker etal., 1981). The'asbestiform fibers to this misconception, asbestiform fibers of other minerals are neglected, and their potential carcinogenic properties

Ahmad, Sajjad

354

Association Nashville, TN  

E-print Network

/8306153/Sunday- Telegraph-at-50-what-will-the-world-be- like-in-another-50-years.html #12;Source: Syd Mead, www-Optimist Views Transhumanism (h+) Pic of kurzweil?pic of ,,when humans transcend...? Pic of movie? Exponential: Cryptocurrency Cryptocurrencies are proliferating! What would happen if the government: � Issued money

Berleant, Daniel

355

Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial  

SciTech Connect

Purpose: The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials: DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the logrank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray's test. Results: Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions: TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories.

Gunderson, Leonard L., E-mail: gunderson.leonard@mayo.edu [Mayo Clinic Cancer Center, Scottsdale, Arizona (United States); Moughan, Jennifer [Radiation Therapy Oncology Group, Philadelphia, Pennsylvania (United States); Ajani, Jaffer A. [The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Pedersen, John E. [Cross Cancer Institute, Edmonton, Alberta (Canada); Winter, Kathryn A. [Radiation Therapy Oncology Group, Philadelphia, Pennsylvania (United States); Benson, Al B. [Northwestern University, Chicago, Illinois (United States); Thomas, Charles R. [Knight Cancer Institute/Oregon Health and Science University, Portland, Oregon (United States); Mayer, Robert J. [Dana-Farber Cancer Institute, Boston, Massachusetts (United States); Haddock, Michael G. [Mayo Clinic Cancer Center, Rochester, Minnesota (United States); Rich, Tyvin A. [University of Virginia, Charlottesville, Virginia (United States); Willett, Christopher G. [Duke University, Durham, North Carolina (United States)

2013-11-15

356

24 CFR 576.301 - Metropolitan cities and urban counties.  

Code of Federal Regulations, 2013 CFR

... 2013-04-01 false Metropolitan cities and urban counties. 576.301 Section...Reallocations 576.301 Metropolitan cities and urban counties. Grant funds returned by a metropolitan city or urban county will be reallocated...

2013-04-01

357

24 CFR 576.301 - Metropolitan cities and urban counties.  

... 2013-04-01 true Metropolitan cities and urban counties. 576.301 Section...Reallocations 576.301 Metropolitan cities and urban counties. Grant funds returned by a metropolitan city or urban county will be reallocated...

2014-04-01

358

24 CFR 576.301 - Metropolitan cities and urban counties.  

Code of Federal Regulations, 2012 CFR

... 2012-04-01 false Metropolitan cities and urban counties. 576.301 Section...Reallocations 576.301 Metropolitan cities and urban counties. Grant funds returned by a metropolitan city or urban county will be reallocated...

2012-04-01

359

78 FR 49446 - Lyon-Mineral County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013

...Lyon-Mineral County Resource Advisory...SUMMARY: The Lyon-Mineral County Resource Advisory...in Yerrington, Nevada. The RAC is meeting...Meeting Room, Lyon County Administration...Street, Yerington, Nevada. Written...

2013-08-14

360

State and County Exposure Levels to I-131  

Cancer.gov

State and County Exposure Levels Per capita thyroid doses resulting from all exposure routes from all tests State and County Exposure Levels Per capita thyroid doses resulting from all exposure routes from all tests Next Section > State and County Exposure

361

7 CFR 780.8 - County committee appeals.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 2011-01-01 false County committee appeals. 780.8 Section...PROGRAMS APPEAL REGULATIONS 780.8 County committee appeals. (a) A request for appeal to a county committee concerning a decision of a...

2011-01-01

362

7 CFR 1221.205 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 false Farm Service Agency County Committee. 1221.205 Section 1221.205...Definitions 1221.205 Farm Service Agency County Committee. Farm Service Agency County Committee, also referred to as FSA...

2012-01-01

363

7 CFR 714.43 - Recommendation by county committee.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 false Recommendation by county committee. 714.43 Section 714...COLLECTED 714.43 Recommendation by county committee. Immediately upon receipt...shall be recorded on the face thereof. The county committee shall determine, on the...

2012-01-01

364

7 CFR 1230.634 - FSA county office report.  

Code of Federal Regulations, 2012 CFR

... 2012-01-01 2012-01-01 false FSA county office report. 1230.634 Section 1230...of Referendum Referendum 1230.634 FSA county office report. The FSA county office will notify the FSA State office of...

2012-01-01

365

7 CFR 1220.604 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 false Farm Service Agency County Committee. 1220.604 Section 1220.604...Definitions 1220.604 Farm Service Agency County Committee. Farm Service Agency County Committee, also referred to as FSA...

2012-01-01

366

9 CFR 205.207 - Amount and County or parish.  

Code of Federal Regulations, 2012 CFR

...Opinions 205.207 Amount and County or parish. (a) The amount of farm products and county or parish, on an EFS and on...and master list entry will identify each county or parish in the same State...

2012-01-01

367

7 CFR 760.902 - Eligible counties and disaster periods.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 2011-01-01 false Eligible counties and disaster periods. 760.902 Section...Livestock Indemnity Program 760.902 Eligible counties and disaster periods. Counties are eligible for agricultural...

2011-01-01

368

7 CFR 1220.626 - FSA county office report.  

Code of Federal Regulations, 2011 CFR

... 2011-01-01 2011-01-01 false FSA county office report. 1220.626 Section 1220.626...a Referendum Definitions 1220.626 FSA county office report. The county FSA office report shall be certified as...

2011-01-01

369

7 CFR 760.902 - Eligible counties and disaster periods.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 2012-01-01 false Eligible counties and disaster periods. 760.902 Section...Livestock Indemnity Program 760.902 Eligible counties and disaster periods. Counties are eligible for agricultural...

2012-01-01

370

7 CFR 7.25 - County executive director duties.  

Code of Federal Regulations, 2012 CFR

... 2012-01-01 2012-01-01 false County executive director duties. 7.25 Section...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.25 County executive director duties. (a) The...

2012-01-01

371

7 CFR 780.8 - County committee appeals.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 2010-01-01 false County committee appeals. 780.8 Section...PROGRAMS APPEAL REGULATIONS 780.8 County committee appeals. (a) A request for appeal to a county committee concerning a decision of a...

2010-01-01

372

7 CFR 760.902 - Eligible counties and disaster periods.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 2010-01-01 false Eligible counties and disaster periods. 760.902 Section...Livestock Indemnity Program 760.902 Eligible counties and disaster periods. Counties are eligible for agricultural...

2010-01-01

373

7 CFR 1280.606 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 false Farm Service Agency County Committee. 1280.606 Section 1280.606...Definitions 1280.606 Farm Service Agency County Committee. Farm Service Agency County Committee , also referred to as FSA...

2012-01-01

374

7 CFR 1230.606 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 false Farm Service Agency County Committee. 1230.606 Section 1230...Definitions 1230.606 Farm Service Agency County Committee. The term Farm Service Agency County Committee, also referred to as the...

2010-01-01

375

7 CFR 1280.629 - FSA county office report.  

Code of Federal Regulations, 2010 CFR

... 2010-01-01 2010-01-01 false FSA county office report. 1280.629 Section 1280.629...a Referendum Definitions 1280.629 FSA county office report. The county FSA office report shall be certified as...

2010-01-01

376

7 CFR 1962.16 - Accounting by County Supervisor.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 false Accounting by County Supervisor. 1962.16 Section 1962...Security 1962.16 Accounting by County Supervisor. The Agency will maintain...instruments. (b) Dispositions. The County Supervisor will record all...

2011-01-01

377

7 CFR 1220.604 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 false Farm Service Agency County Committee. 1220.604 Section 1220.604...Definitions 1220.604 Farm Service Agency County Committee. Farm Service Agency County Committee, also referred to as FSA...

2011-01-01

378

7 CFR 714.43 - Recommendation by county committee.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 false Recommendation by county committee. 714.43 Section 714...COLLECTED 714.43 Recommendation by county committee. Immediately upon receipt...shall be recorded on the face thereof. The county committee shall determine, on the...

2010-01-01

379

7 CFR 1221.229 - FSA county office report.  

Code of Federal Regulations, 2012 CFR

... 2012-01-01 2012-01-01 false FSA county office report. 1221.229 Section 1221.229...of Referenda Procedures 1221.229 FSA county office report. The county FSA office report shall be certified as...

2012-01-01

380

7 CFR 1280.606 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 false Farm Service Agency County Committee. 1280.606 Section 1280.606...Definitions 1280.606 Farm Service Agency County Committee. Farm Service Agency County Committee , also referred to as FSA...

2010-01-01

381

7 CFR 1230.606 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 false Farm Service Agency County Committee. 1230.606 Section 1230...Definitions 1230.606 Farm Service Agency County Committee. The term Farm Service Agency County Committee, also referred to as the...

2011-01-01

382

7 CFR 1221.205 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 false Farm Service Agency County Committee. 1221.205 Section 1221.205...Definitions 1221.205 Farm Service Agency County Committee. Farm Service Agency County Committee, also referred to as FSA...

2011-01-01

383

7 CFR 1220.604 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 false Farm Service Agency County Committee. 1220.604 Section 1220.604...Definitions 1220.604 Farm Service Agency County Committee. Farm Service Agency County Committee, also referred to as FSA...

2010-01-01

384

7 CFR 1230.634 - FSA county office report.  

Code of Federal Regulations, 2011 CFR

... 2011-01-01 2011-01-01 false FSA county office report. 1230.634 Section 1230...of Referendum Referendum 1230.634 FSA county office report. The FSA county office will notify the FSA State office of...

2011-01-01

385

7 CFR 1280.629 - FSA county office report.  

Code of Federal Regulations, 2011 CFR

... 2011-01-01 2011-01-01 false FSA county office report. 1280.629 Section 1280.629...a Referendum Definitions 1280.629 FSA county office report. The county FSA office report shall be certified as...

2011-01-01

386

7 CFR 1221.229 - FSA county office report.  

Code of Federal Regulations, 2011 CFR

... 2011-01-01 2011-01-01 false FSA county office report. 1221.229 Section 1221.229...of Referenda Procedures 1221.229 FSA county office report. The county FSA office report shall be certified as...

2011-01-01

387

7 CFR 1230.634 - FSA county office report.  

Code of Federal Regulations, 2010 CFR

... 2010-01-01 2010-01-01 false FSA county office report. 1230.634 Section 1230...of Referendum Referendum 1230.634 FSA county office report. The FSA county office will notify the FSA State office of...

2010-01-01

388

7 CFR 1280.629 - FSA county office report.  

Code of Federal Regulations, 2012 CFR

... 2012-01-01 2012-01-01 false FSA county office report. 1280.629 Section 1280.629...a Referendum Definitions 1280.629 FSA county office report. The county FSA office report shall be certified as...

2012-01-01

389

7 CFR 1962.16 - Accounting by County Supervisor.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 false Accounting by County Supervisor. 1962.16 Section 1962...Security 1962.16 Accounting by County Supervisor. The Agency will maintain...instruments. (b) Dispositions. The County Supervisor will record all...

2012-01-01

390

7 CFR 714.43 - Recommendation by county committee.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 false Recommendation by county committee. 714.43 Section 714...COLLECTED 714.43 Recommendation by county committee. Immediately upon receipt...shall be recorded on the face thereof. The county committee shall determine, on the...

2011-01-01

391

7 CFR 1962.16 - Accounting by County Supervisor.  

Code of Federal Regulations, 2010 CFR

... 2009-01-01 true Accounting by County Supervisor. 1962.16 Section 1962...Security 1962.16 Accounting by County Supervisor. The Agency will maintain...instruments. (b) Dispositions. The County Supervisor will record all...

2010-01-01

392

7 CFR 1230.606 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 false Farm Service Agency County Committee. 1230.606 Section 1230...Definitions 1230.606 Farm Service Agency County Committee. The term Farm Service Agency County Committee, also referred to as the...

2012-01-01

393

7 CFR 1220.626 - FSA county office report.  

Code of Federal Regulations, 2010 CFR

... 2010-01-01 2010-01-01 false FSA county office report. 1220.626 Section 1220.626...a Referendum Definitions 1220.626 FSA county office report. The county FSA office report shall be certified as...

2010-01-01

394

7 CFR 780.8 - County committee appeals.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 2012-01-01 false County committee appeals. 780.8 Section...PROGRAMS APPEAL REGULATIONS 780.8 County committee appeals. (a) A request for appeal to a county committee concerning a decision of a...

2012-01-01

395

7 CFR 7.25 - County executive director duties.  

Code of Federal Regulations, 2011 CFR

... 2011-01-01 2011-01-01 false County executive director duties. 7.25 Section...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.25 County executive director duties. (a) The...

2011-01-01

396

9 CFR 205.207 - Amount and County or parish.  

Code of Federal Regulations, 2011 CFR

...Opinions 205.207 Amount and County or parish. (a) The amount of farm products and county or parish, on an EFS and on...and master list entry will identify each county or parish in the same State...

2011-01-01

397

7 CFR 1220.626 - FSA county office report.  

Code of Federal Regulations, 2012 CFR

... 2012-01-01 2012-01-01 false FSA county office report. 1220.626 Section 1220.626...a Referendum Definitions 1220.626 FSA county office report. The county FSA office report shall be certified as...

2012-01-01

398

7 CFR 7.25 - County executive director duties.  

Code of Federal Regulations, 2010 CFR

... 2010-01-01 2010-01-01 false County executive director duties. 7.25 Section...AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES 7.25 County executive director duties. (a) The...

2010-01-01

399

7 CFR 1280.606 - Farm Service Agency County Committee.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 false Farm Service Agency County Committee. 1280.606 Section 1280.606...Definitions 1280.606 Farm Service Agency County Committee. Farm Service Agency County Committee , also referred to as FSA...

2011-01-01

400

77 FR 9652 - Environmental Impacts Statements; Notice of Availability  

Federal Register 2010, 2011, 2012, 2013

...602-417-9235. EIS No. 20120034, Draft EIS, USFS, CA, Harris Vegetation Management Project, To Improve Forest Health and...Garfield, Iron, Juab, Millard, Piute, Sanpete, Sevier, and Wayne Counties, UT, Comment Period Ends: 04/02/2012,...

2012-02-17

401

Homelessness and Substance Use in Cook County  

E-print Network

and Substance Abuse State of Illinois by Timothy P. Johnson and Mark E. Barrett Survey Research Laboratory University of Illinois September 1991 #12;#12;Homelessness & Substance Abuse in Cook County i CONTENTS Page ....................................................................................................49 #12;Homelessness & Substance Abuse in Cook County ii CONTENTS--Continued Page REFERENCES

Illinois at Chicago, University of

402

Asbestos Emission Control Plan Dakota County, Minnesota  

E-print Network

Asbestos Emission Control Plan UMore Park Dakota County, Minnesota Prepared for University of Minnesota Revised: July 22, 2009 UMP005460 #12;Asbestos Emission Control Plan UMore Park Dakota County park environmental\\WorkFiles\\Phase II Investigation WO#1 and #6\\Phase II Work Plan\\Asbestos ECP\\ECP_V2

Netoff, Theoden

403

Analysis of County School Districts in Arkansas.  

ERIC Educational Resources Information Center

The 1948, Arkansas School District Reorganization Act was passed in an effort to reduce the 1589 small school districts to a smaller number. Those districts not consolidated would form county districts. As of the 1967-68 school year, 26 of these county districts remained. The purpose of this study was to provide information drawing attention to

Budd, Karol B.; Charlton, J.L.

404

County Data Book 1997: Kentucky Kids Count.  

ERIC Educational Resources Information Center

This Kids Count data book examines trends in the well-being of Kentucky's children on a statewide and county basis. An introduction summarizes some of the trends for Kentucky's children in the 1990s. The bulk of the report presents statewide and county data grouped into five categories: (1) poverty rates and programs (persons in poverty; median

Kentucky Kids Count Consortium.

405

COUNTIES OF CALIFORNIA - WITH FARM STATISTICS  

EPA Science Inventory

County farming statistics polygon coverage of California. The statistics were taken from the 1997 Census of Agriculture and added to a coverage extracted from TIGER county boundaries. A farm is any place from which $1,000 or more of agricultural products were sold, or normally w...

406

Pennsylvania Dutch Country of Lancaster County  

NSDL National Science Digital Library

The Pennsylvania Dutch Convention And Visitors Bureau, in cooperation with the attractions of the Amish Country of Lancaster County, Pennsylvania, now offer a complete virtual information center on the Pennsylvania Dutch Country of Lancaster County. Includes information on the Amish, quilts, covered bridges, and all the interesting things to see and do in the area.

407

Socioeconomic Heterogeneity of Mining-Dependent Counties.  

ERIC Educational Resources Information Center

Although the socioeconomic well-being of all U.S. mining-dependent counties was slightly above the national average in 1990, disaggregation reveals substantial effects of region and mining subsector. In particular, southern and Great Lakes coal-mining counties had significantly lower high school graduation rates and higher poverty and unemployment

Nord, Mark; Luloff, A. E.

1993-01-01

408

Educational and Demographic Profile: San Benito County.  

ERIC Educational Resources Information Center

This profile uniquely presents a variety of educational and socioeconomic information for San Benito County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced

California Postsecondary Education Commission, 2004

2004-01-01

409

Public Library Service for San Benito County.  

ERIC Educational Resources Information Center

A sparsely populated, agricultural area, San Benito County (California) provides library services in conjunction with the Hollister city library and in cooperation with the San Juan Bautista city library. Financing comes from the county general fund. There are no written goals or policy statements and no professionally trained librarians. As

McGovern, Gail

410

SUFFOLK COUNTY DEPARTMENT OF HEALTH SERVICES  

E-print Network

of Environmental Conservation has recognized conformance withArticle 12 of the Suffolk County Sanitary Code as conformance with the New York State Petroleum Bulk Storage Law. Any facility located in Suffolk County which, Illinois 60062. b. "API 650" means American Petroleum Institute, Standard 650, Welded Steel Tanks for Oil

411

Mn/DOT County Road Safety Plans  

E-print Network

. !! Position counties to compete for safety funds !! Highway Safety Improvement Program !! High Risk Rural Roads Program !! Minnesota Central Safety Funds !! Foster safety culture among county stakeholders 4,073 ­ 24% 2,242 ­ 39% Rural 24,474 ­ 63% 1,860 ­ 83% Urban 14,599 ­ 37% 382 ­ 17% All Way Stop 438 ­ 6% 6

Minnesota, University of

412

County Data Book 1995: Kentucky Kids Count.  

ERIC Educational Resources Information Center

This data book presents findings of the Kids Count project on current conditions faced by Kentucky children age birth through 19. For each county, and for the state, comparisons are provided between the base years of 1980-1982 and the most recent years 1992-1994. Counties are ranked against each other and trend graphs are provided for the studied

Kentucky Youth Advocates, Inc., Louisville.

413

Crawford County Adult Education Center Program Evaluation  

ERIC Educational Resources Information Center

The Crawford County Adult Education Program Evaluation was implemented to gather data that would assist in increasing the number of enrolled students at CCAEC. The data were compiled from state, federal, local extant data, a Community Opinion Survey and focus group interviews. Of the 1,015 Crawford County Residents who were emailed the COS, 213

Faubus, Deborah L.

2011-01-01

414

Public Health County of Santa Cruz  

E-print Network

Public Health Division County of Santa Cruz HEALTH SERVICES AGENCY POST OFFICE BOX 962, 1080 at the two Public Health clinics located at 1080 Emeline Ave. in Santa Cruz and at 9 Crestview Dr. in Watsonville, on a walk-in basis. County health clinic services are limited to those who reside in Santa Cruz

California at Santa Cruz, University of

415

COUNTY BOUNDARIES FOR THE MAIA STUDY REGION  

EPA Science Inventory

This data set is a geographic information system (GIS) coverage of the county boundaries in the United States Environmental Protection Agency (USEPA) Mid-Atlantic Integrated Assessment (MAIA) Project region. The coverage was produced using the US Geological Survey county bound...

416

Geothermal development issues: Recommendations to Deschutes County  

SciTech Connect

This report discusses processes and issues related to geothermal development. It is intended to inform planners and interested individuals in Deschutes County about geothermal energy, and advise County officials as to steps that can be taken in anticipation of resource development. (ACR)

Gebhard, C.

1982-07-01

417

A Profile of Anson County, North Carolina.  

ERIC Educational Resources Information Center

Since 1950 Anson County, North Carolina, has had major contributions to economic development, a source of great concern to residents of the almost entirely rural area. The increased capacity of the Blewitt Falls Dam power output and the county-wide water filtration system (one of only a few in the United States today) are attractive to industry.

Farr, M. Gaston; And Others

418

Commodity Flow Study - Clark County, Nevada, USA  

SciTech Connect

The United States Department of Energy has designated Clark County, Nevada as an 'Affected Unit of Local Government' due to the potential for impacts by activities associated with the Yucca Mountain High Level Nuclear Waste Repository project. Urban Transit, LLC has led a project team of transportation including experts from the University of Nevada Las Vegas Transportation Research Center to conduct a hazardous materials community flow study along Clark County's rail and truck corridors. In addition, a critical infrastructure analysis has also been carried out in order to assess the potential impacts of transportation within Clark County of high level nuclear waste and spent nuclear fuel to a proposed repository 90 miles away in an adjacent county on the critical infrastructure in Clark County. These studies were designed to obtain information relating to the transportation, identification and routing of hazardous materials through Clark County. Coordinating with the United States Department of Energy, the U.S. Department of Agriculture, the U. S. Federal Highway Administration, the Nevada Department of Transportation, and various other stakeholders, these studies and future research will examine the risk factors along the entire transportation corridor within Clark County and provide a context for understanding the additional vulnerability associated with shipping spent fuel through Clark County. (authors)

Conway, S.Ph.D. [Urban Environmental Research LLC, Las Vegas, NV (United States); Navis, I. [AICP Planning Manager, Clark County Nuclear Waste Division, Department of Comprehensive Planning, Las Vegas, NV (United States)

2008-07-01

419

Employment concentration across U.S. counties  

Microsoft Academic Search

This paper examines the spatial distribution of jobs across U.S. counties between 1970 and 2000, and investigates whether sectoral employment is becoming more or less concentrated. The existing literature has found deconcentration (convergence) of employment across urban areas. Cities only cover a small part of the U.S. though. Using county data, our results indicate that deconcentration is limited to the

Klaus Desmet; Marcel Fafchamps

2006-01-01

420

Tennessee County Higher Education Profiles, 2010  

ERIC Educational Resources Information Center

The "Tennessee County Higher Education Profiles 2010" provide basic information with respect to higher education for each county in Tennessee. The 2010 "Profiles" represent the fourth year for this report. In the demographic and enrollment sections, a few changes were made to better meet the needs of policymakers. This document explains those

Tennessee Higher Education Commission, 2010

2010-01-01

421

Do's & Don'ts Manatee County  

E-print Network

Fertilizer Ordinance Do's & Don'ts Manatee County Things to look for in your lawn maintenance around water bodies and wetlands? For more information please contact us at: MANATEE COUNTY Division of Ag & Extension Service 941.722.4524 http://manatee.ifas.ufl.edu email manatee@ifas.ufl.edu ORDINANCE

Jawitz, James W.

422

Identification and Characterization of Non-Cellulose-Producing Mutants of Gluconacetobacter hansenii Generated by Tn5 Transposon Mutagenesis  

PubMed Central

The acs operon of Gluconacetobacter is thought to encode AcsA, AcsB, AcsC, and AcsD proteins that constitute the cellulose synthase complex, required for the synthesis and secretion of crystalline cellulose microfibrils. A few other genes have been shown to be involved in this process, but their precise role is unclear. We report here the use of Tn5 transposon insertion mutagenesis to identify and characterize six non-cellulose-producing (Cel?) mutants of Gluconacetobacter hansenii ATCC 23769. The genes disrupted were acsA, acsC, ccpAx (encoding cellulose-complementing protein [the subscript Ax indicates genes from organisms formerly classified as Acetobacter xylinum]), dgc1 (encoding guanylate dicyclase), and crp-fnr (encoding a cyclic AMP receptor protein/fumarate nitrate reductase transcriptional regulator). Protein blot analysis revealed that (i) AcsB and AcsC were absent in the acsA mutant, (ii) the levels of AcsB and AcsC were significantly reduced in the ccpAx mutant, and (iii) the level of AcsD was not affected in any of the Cel? mutants. Promoter analysis showed that the acs operon does not include acsD, unlike the organization of the acs operon of several strains of closely related Gluconacetobacter xylinus. Complementation experiments confirmed that the gene disrupted in each Cel? mutant was responsible for the phenotype. Quantitative real-time PCR and protein blotting results suggest that the transcription of bglAx (encoding ?-glucosidase and located immediately downstream from acsD) was strongly dependent on Crp/Fnr. A bglAx knockout mutant, generated via homologous recombination, produced only ?16% of the wild-type cellulose level. Since the crp-fnr mutant did not produce any cellulose, Crp/Fnr may regulate the expression of other gene(s) involved in cellulose biosynthesis. PMID:24013627

Deng, Ying; Nagachar, Nivedita; Xiao, Chaowen; Tien, Ming

2013-01-01

423

Impacts of cool cities on air quality: A preliminary modeling assessment for Nashville TN, Dallas TX and Atlanta GA  

SciTech Connect

Previous atmospheric modeling efforts that concentrated on the Los Angeles Basin suggested beneficial and significant air quality impacts from cool cities strategies. This paper discusses an extension of similar modeling efforts to three regions, Atlanta GA, Dallas - Ft. Worth TX, and Nashville TN, that experience smog and air quality problems. According to the older ozone air quality standard (120 ppb), these regions were classified as serious, moderate, and marginal, respectively, but may be out of compliance with respect to the newer, 80-ppb/8-hours standard. Results from this exploratory modeling work suggest a range of possible impacts on meteorological and air quality conditions. For example, peak ozone concentrations during each region's respective episode could be decreased by 1-6 ppb (conservative and optimistic scenarios, respectively) in Nashville, 5-15 ppb in Dallas - Fort Worth, and 5-12 ppb in Atlanta following implementation of cool cities. The reductions are generally smaller than those obtained from simulating the Los Angeles Basin but are still significant. In all regions, the simulations suggest, the net, domain-wide effects of cool cities are reductions in ozone mass and improvements in air quality. In Atlanta, Nashville, and Dallas, urban areas benefiting from reduced smog reach up to 8460, 7350, and 12870 km{sup 2} in area, respectively. Results presented in this paper should be taken as exploratory and preliminary. These will most likely change during a more comprehensive modeling study to be started soon with the support of the US Environmental Protection Agency. The main purpose of the present project was to obtain the initial data (emission inventories) for these regions, simulate meteorological conditions, and perform preliminary sensitivity analysis. In the future, additional regions will be simulated to assess the potential of cool cities in improving urban air quality.

Taha, Haider

1998-06-15

424

77 FR 59573 - Proposed Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri...  

Federal Register 2010, 2011, 2012, 2013

...Class E airspace extending upward from 700 feet above the surface at Hawkins County Airport...controlled airspace extending upward from 700 feet above the surface at Tri-Cities Airport...Class E airspace extending upward from 700 feet above the surface at Hawkins County...

2012-09-28

425

SCORE Science: Humboldt County Office of Education  

NSDL National Science Digital Library

SCORE, a cooperative project of the California County Superintendent's Educational Services Association, has made K-12 teacher and student resources in four major subject areas available at sites in Humboldt County (Science), San Diego County (Language Arts), Kings County (Mathematics), and San Bernardino and Butte Counties (Social Studies). While each site has its own personality and delivery style, they all make an attempt to break out teacher and/or student resources by grade level. In some cases (Science, Social Studies) this is done through a search interface. In others (Language Arts, Mathematics) it is done via a browsable interface. It is this categorization, plus the fact that resources are selected, evaluated, and annotated by teachers, that is the both the power and unifying theme of the sites. Depending on the site, assessment and teacher development sections may also be available.

1996-01-01

426

Environmental assessment: Deaf Smith County site, Texas  

SciTech Connect

In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of nine potentially acceptable sites for a mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and the eight other potentially sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines. On the basis of these findings, the DOE is nominating the Deaf Smith County site as one of the five sites suitable for characterization. 591 refs., 147 figs., 173 tabs.

Not Available

1986-05-01

427

Shoreham Railroad Bridge, Former Addison County Railroad (later, Rutland Railroad, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Shoreham Railroad Bridge, Former Addison County Railroad (later, Rutland Railroad, Addison Branch), spanning Lemon Fair River above Richville Pond, west of East Shoreham Road, Shoreham, Addison County, VT

428

7. Photocopy of photograph (from Broome County Historical Society) showing ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photocopy of photograph (from Broome County Historical Society) showing SWIMMERS, PHOTOGRAPH TAKEN FACING NORTHEAST - Charles F. Johnson Pool, Charles F. Johnson Park, Johnson City, Broome County, NY

429

Neutron scattering measurements of spatially anisotropic magnetic exchange interactions in semiconducting K0.85 Fe1.54Se2 (TN = 280 K.  

PubMed

We use neutron scattering to study the spin excitations associated with the stripe antiferromagnetic order in semiconducting K(0.85)Fe(1.54)Se(2) (T(N) = 280 K). We show that the spin-wave spectra can be accurately described by an effective Heisenberg Hamiltonian with highly anisotropic inplane couplings at T = 5 K. At high temperature (T = 300 K) above T(N), short-range magnetic correlation with anisotropic correlation lengths are observed. Our results suggest that, despite the dramatic difference in the Fermi surface topology, the inplane anisotropic magnetic couplings are a fundamental property of the iron-based compounds; this implies that their antiferromagnetism may originate from local strong correlation effects rather than weak coupling Fermi surface nesting. PMID:24836268

Zhao, Jun; Shen, Yao; Birgeneau, R J; Gao, Miao; Lu, Zhong-Yi; Lee, D-H; Lu, X Z; Xiang, H J; Abernathy, D L; Zhao, Y

2014-05-01

430

Neutron Scattering Measurements of Anisotropic Magnetic Exchange Interactions in Semiconducting K0.85Fe1.54Se2 (TN=280K)  

SciTech Connect

We use neutron scattering to study the spin excitations associated with the stripe antiferromagnetic (AFM) order in semiconducting K0.85Fe1.54Se2 (TN=280 K). We show that the spin wave spectra can be accurately described by an effective Heisenberg Hamiltonian with highly anisotropic in-plane couplings at T= 5 K. At high temperature (T= 300 K) above TN, short range magnetic correlation with anisotropic correlation lengths are observed. Our results suggest that, despite the dramatic difference in the Fermi surface topology, the in-plane anisotropic magnetic coupings are a fundamental property of the iron based compounds; this implies that their antiferromagnetism may originate from local strong correlation effects rather than weak coupling Fermi surface nesting.

Abernathy, Douglas L [ORNL] [ORNL; Birgeneau, R. J. [University of California, Berkeley] [University of California, Berkeley; Gao, Miao [Renmin University of China] [Renmin University of China; Lu, Zhong-Yi [Renmin University of China] [Renmin University of China; Zhao, Jun [Fudan University, China] [Fudan University, China; Zhao, Y. [NIST and Univ. of MD] [NIST and Univ. of MD

2014-01-01

431

Genetic linkage of the vanB2 gene cluster to Tn5382 in vancomycin-resistant enterococci and characterization of two novel insertion sequences  

Microsoft Academic Search

VanB-type vancomycin resistance is encoded by the vanB gene cluster, which disseminates by horizontal gene transfer and clonal spread of vancomycin- resistant enterococci (VRE). Genetic linkage of the vanB gene cluster to transposon Tn5382 and the insertion sequences IS16 and IS256-like has previously been shown. In this study linkage of defined vanB gene cluster subtypes to these elements was examined.

Kristin H. Dahl; Eirik W. Lundblad; Torunn P. Rkenes; rjan Olsvik; Arnfinn Sundsfjord

432

Effect of the polymer concentration on the ON/OFF states of a TN-LCD: polyvinyl alcohol vs. soy lecithin  

NASA Astrophysics Data System (ADS)

In this work we study the response of a Twisted Nematic Liquid Crystal Display (TN-LCD) by varying both the concentration and the polymer used for the microgroove. We compare the performance of two polymers: polyvinyl alcohol and soy lecithin. In particular, the light transmission for the ON/OFF states is evaluated. The polyvinyl alcohol is a polymer widely used in LCDs while lecithin soy is a natural polymer.

de Coss Martinez, Romeo; Gonzalez Murguia, Jose Luis

2011-03-01

433

Prevalence of aac(6?)-Ie-aph(2?)-Ia resistance gene and its linkage to Tn5281 in Enterococcus faecalis and Enterococcus faecium isolates from Tabriz hospitals  

PubMed Central

Background and Objective High-level gentamicin resistance (HLGR: MIC ? 500 g/ml) in Enterococci is mediated by aminoglycoside modifying enzymes which is mainly encoded by aac(6?)-Ie-aph(2?)-Ia gene. The aim of this study was to evaluate the frequency of aac(6?)-Ie-aph(2?)-Ia gene in clinical isolates of Enterococcus facium and Enterococcus faecalis collected from hospitals in northwest of Iran. Materials and methods In the present study a total of 111 enterococcus isolates were collected from 4 hospitals during a two year period (July 2009-August 2011). Bacterial identification and species determination were carried out by standard biochemical tests. Antimicrobial susceptibility was evaluated by Kirby Bauer disc diffusion method. MICs were determined by agar dilution method. The frequency of aac(6?)-Ie-aph(2?)-Ia gene in the isolates was determined by PCR. The carriage of resistance gene on Tn5281 transposon was identified by long PCR and dot-blot hybridization methods. Results Antibiotic susceptibility tests revealed that the highest resistance was against streptomycin (74.77%) and erythromycin (67.58%) whereas the highest susceptibility was observed to vancomycin (81.1%). 36 isolates (32.43%) were identified as HLGR, 34(94.44%) of them had resistant gene in their genome. Long PCR studies revealed that 88% of HLGR clinical isolates harboured Tn5281. The aac(6?)-Ie-aph(2?)-Ia resistance gene was present on Tn5281 transposon in all 32 isolates according to dot blot hybridization test. Conclusion The results of this study indicated that aac(6?)-Ie-aph(2?)-Ia resistance gene is highly prevalent in gentamicin resistant isolates. Carriage of aac(6?)-Ie-aph(2?)-Ia resistance gene on Tn5281 transposable element suggests possible contribution of this transposone on dissemination of resistance gene among enterococcus isolates. PMID:24475324

Behnood, Amir; Farajnia, Safar; Moaddab, Seyed Reza; Ahdi-Khosroshahi, Shiva; Katayounzadeh, Aliakbar

2013-01-01

434

Transductional mapping of ksgB and a new Tn5-induced kasugamycin resistance gene, ksgD, in Escherichia coli K-12.  

PubMed

We have mapped the Escherichia coli ksgB gene to min 36.5, 0.8 min from man and 0.7 min from aroD. A new kasugamycin resistance (Ksgr) gene, ksgD, has been isolated, using a transposon, Tn5. ksgD::TN5 is 44% cotransducible with sbcA, unlinked to trp, and unlinked to man (by P1 transduction). The ksgD::Tn5 has a late time of entry from HfrB7 (PO43). These data place ksgD clockwise from sbcA (which enters early from HfrB7) at min 30.4. The reistance of ksgB ksgD single and double mutant strains has been quantitated. Single mutations, ksgB or ksgD, gave resistance to 600 micrograms of kasugamycin per ml, whereas a ksgB ksgD strain was able to grow in the presence of kasugamycin levels in excess of 3,000 micrograms/ml. This indicates that the mechanisms of resistance coded for by the two genes are independent and synergistic. PMID:6257656

Fouts, K E; Barbour, S D

1981-02-01

435

Knox County Black History Archives  

NSDL National Science Digital Library

Knox County, Ohio has had a significant black population for several hundred years, and historians and others have documented their activities in journal articles, books, and other printed materials. This remarkable digital collection from the Digital Resource Commons at Kenyon College provides access to photographs, documents, and other items that tell the story of this unique community. This collection was made possible, in part, by funding from the Ohio Humanities Council, along with support from the Rural Life Center. Currently the collection includes 200 items, which visitors can browse alphabetically or by performing a keyword search. Each item has a preview feature and visitors can click through to zoom in on each item. The handwritten history of Mt. Cavalry Baptist Church is particularly fascinating.

2012-06-01

436

Hydrologic conditions: Dade County, Florida  

USGS Publications Warehouse

Thin layers of dense limestone of low permeability that occur near the top of the Biscayne aquifer in the vicinity of the north end of Levee 30 in Dade County, Florida are of hydrologic importance because they retard the downward infiltration of ponded water in Conservation Area No. 3. This retarding effect frequently results in high head differentials across the levee. Tests made in a small area adjacent to Levee 30 indicate that the coefficient of transmissibility of the aquifer is 3,600,000 gpd (gallons per day) per foot, and the coefficient of vertical permeability of the dense limestones is 13 gpd per square foot. If ground-water flow beneath the levee is laminar, the total inflow to the Levee 30 Canal from Conservation Area No. 3 will be about 350 mgd (million gallons per day), or 540 cfs (cubic feet per second), per mile length of levee when the head difference across the levee is 10 feet.

Kohout, Francis Anthony; Klein, Howard; Sherwood, C. B.; Leach, Stanley D.

1964-01-01

437

Analysis of the Shallow Groundwater Flow System at Fire Island National Seashore, Suffolk County, New York  

USGS Publications Warehouse

Fire Island National Seashore (FIIS) occupies 42 kilometers of the barrier island for which it is named that lies off the southern shore of Suffolk County, N.Y. Freshwater in the highly permeable, sandy aquifer underlying Fire Island is bounded laterally by marine surface waters and at depth by saline groundwater. Interspersed throughout FIIS are 17 pre-existing residential communities that in summer months greatly increase in population through the arrival of summer residents and vacationers; in addition, the National Park Service (NPS) has established several facilities on the island to accommodate visitors to FIIS. The 2.2 million people estimated by the NPS to visit Fire Island annually impact groundwater quality through the release of waste-derived contaminants, such as nutrients, pathogens, and organic compounds, into the environment. Waste-contaminated groundwater can move through the aquifer and threaten the ecological health of the adjacent back-barrier estuaries to which much of the groundwater ultimately discharges. In 2004, the U.S. Geological Survey (USGS), in cooperation with the NPS, began a 3-year investigation to (1) collect groundwater levels and water-quality (nutrient) samples, (2) develop a three-dimensional model of the shallow (water-table) aquifer system and adjacent marine surface waters, and (3) calculate nitrogen loads in simulated groundwater discharges from the aquifer to back-barrier estuaries and the ocean. The hydrogeology of the shallow aquifer system was characterized from the results of exploratory drilling, geophysical surveying, water-level monitoring, and water-quality sampling. The investigation focused on four areas-the communities of Kismet and Robbins Rest, the NPS Visitor Center at Watch Hill, and the undeveloped Otis Pike Fire Island High Dune Wilderness. Thirty-five observation wells were installed within FIIS to characterize subsurface hydrogeology and establish a water-table monitoring network in the four study areas. A variable-density model of the shallow aquifer system and adjacent marine surface waters was developed to simulate groundwater flow patterns and rates. Nitrogen loads from the shallow aquifer system were calculated from representative total nitrogen (TN) concentrations and simulated groundwater discharges to back-barrier estuaries and the ocean. The model simulates groundwater directions, velocities, and discharge rates under 2005 mean annual conditions. Groundwater budgets were developed for recharge areas of similar land use that contribute freshwater to back-barrier estuaries, the ocean, and subsea-discharge zones. Total freshwater discharge from the shallow aquifer system is about 43,500 cubic meters per day (m3/d) (79.8 percent) to back-barrier estuaries and about 10,200 m3/d (18.7 percent) to the ocean; about 836 m3/d (1.5 percent) may exit the system as subsea underflow. The total contribution of fresh groundwater to shoreline discharge zones amounts to about 53,700 m3/d (98.5 percent). The median age of freshwater discharged to back-barrier estuaries and the ocean was 3.4 years, and the 95th-percentile age was 20 years. The TN concentrations and loads under 2005 mean annual conditions for areas that contribute fresh groundwater to back-barrier estuaries and the ocean were calculated for the principal land uses on Fire Island. The overall TN load from the shallow aquifer system to shoreline discharge zones is about 16,200 kilograms per year (kg/yr) (82.2 percent) to back-barrier estuaries and about 3,500 kg/yr (17.8 percent) to the ocean. The overall TN load to marine surface waters amounts to about 19,700 kg/yr-roughly 6 percent of the annual TN load from shallow groundwater entering the South Shore Estuary Reserve (SSER) from the Suffolk County mainland, which is about 345,000 kg/yr. In contrast to the TN load from shallow groundwater for the SSER watershed, which annually yields about 353 kilograms per square kilometer (kg/km2), the overall TN loa

Schubert, Christopher E.

2010-01-01

438

Tn5-induced mutants of Azotobacter vinelandii affected in nitrogen fixation under Mo-deficient and Mo-sufficient conditions  

SciTech Connect

Mutants of Azotobacter vinelandii affected in N/sub 2/ fixation in the presence of 1 ..mu..M Na/sub 2/MoO/sub 4/ (conventional system), 50 nM V/sub 2/O/sub 5/, or under Mo deficiency (alternative system) have been isolated after Tn5 mutagenesis with the suicide plasmid pSUP1011. These mutants are grouped into four broad phenotypic classes. Mutants in the first class are Nif/sup -/ under Mo sufficiency but Nif/sup +/ under Mo deficiency or in the presence of V/sub 2/O/sub 5/. Mutants in the second class are Nif/sup -/ under all conditions. An FeMo-cofactor-negative mutant (NifB/sup -/) belongs to this class. The third mutant class consists of mutants incapable of N/sub 2/-dependent growth under Mo deficiency. Most of the mutants of this class are also affected in N/sub 2/ fixation in the presence of 1 ..mu..M Na/sub 2/MoO/sub 4/, with acetylene reduction rates ranging from 28 to 51% of the rates of the wild type. Strains constructed by genetic transfer of the Kan/sup r/ marker of mutants from this class into nifHDK or nifK deletion mutants showed N/sub 2/-dependent growth only in the presence of V/sup 2/O/sub 5/. The only mutant in the fourth class shows wild-type nitrogenase activity under Mo sufficiency, but only 10% of the acetylene reduction activity of the wild type in the presence of 50 nM V/sub 2/O/sub 5/. The acetylene reduction rates of whole cells of this mutant are identical in Mo-deficient medium and in medium containing V/sub 2/O/sub 5/. The conventional nitrogenase subunits are expressed in this mutant even under Mo deficiency or in the presence of V/sub 2/O/sub 5/; however, the NH/sub 4//sup +/-and Mo-repressible proteins normally seen under these conditions could not be detected on two-dimensional gels.

Joerger, R.D.; Premakumar, R.; Bishop, P.E.

1986-11-01

439

1918 Influenza: A Winnebago County, Wisconsin Perspective  

PubMed Central

The population of Winnebago County in 1918 was approximately 62,000 residents. It consisted of towns supporting diverse manufacturers surrounded by farming country. For this study, records were revisited, and 1918 to 1920 influenza survivors were interviewed. A pharmacological investigation encompassing the various influenza treatments used in Wisconsin from 1918 to 1920 was documented. In 1918, over 180 individuals perished from influenza, and over 2000 cases were reported in Winnebago County, Wisconsin. Influenza returned in 1920, which some researchers refer to as the fourth wave, claiming nearly 50 lives in Winnebago County, Wisconsin. This study also documents the 1920 influenza wave. PMID:19889943

Shors, Teri; McFadden, Susan H.

2009-01-01

440

Drainage basins in Duval County, Florida  

USGS Publications Warehouse

The drainage basins and subbasins in Duval County, Florida, are delineated on this atlas map. The county 's 840 square-mile area is drained by three major river systems; the St. Johns, 668 square miles; Nassau, 113 square miles; and St. Marys, 59 square miles. The remainder of the county is drained by a network of small streams that flow into either the Intracoastal Waterway or directly into the Atlantic Ocean. The sub-basins range in size from less than one square mile to more than 50 square miles. (USGS)

Stone, Roy B.; Largen, Joseph B.

1983-01-01

441

Tri-county pilot study. [Texas  

NASA Technical Reports Server (NTRS)

The author has identified the following significant results. An area inventory was performed for three southeast Texas counties (Montgomery, Walker, and San Jacinto) totaling 0.65 million hectares. The inventory was performed using a two level hierarchy. Level 1 was divided into forestland, rangeland, and other land. Forestland was separated into Level 2 categories: pine, hardwood, and mixed; rangeland was not separated further. Results consisted of area statistics for each county and for the entire study site for pine, hardwood, mixed, rangeland, and other land. Color coded county classification maps were produced for the May data set, and procedures were developed and tested.

Reeves, C. A. (principal investigator); Austin, T. W.; Kerber, A. G.

1976-01-01

442

Lea County 001, an H5 chondrite, and Lea County 002, an ungrouped type 3 chondrite  

NASA Technical Reports Server (NTRS)

A search of active deflation basins near Jal, Lea County, New Mexico resulted in the discovery of two meteorites, Lea County 001 and 002. Lea County 001 has mean olivine and low-Ca pyroxene compositions of Fa(19) and Fs(17), respectively. These and all other mineralogical and petrological data collected indicate a classification of H5 for this stone. Lea County 002 has mean olivine and low-Ca pyroxene compositions of Fa(2) and Fs(4), and is unequilibrated. Although it is mineralogically most similar to Kakangari and chondritic clasts within Cumberland Falls, the high modal amount of forsterite makes Lea County a unique type 3 chondrite. Oxygen isotope data for Lea County 002 fall on an 0-16-mixing line through those of the enstatite meteorites and IAB irons, a feature shared by Kakangari.

Zolensky, Michael E.; Score, Roberta; Clayton, Robert N.; Mayeda, Toshiko K.; Schutt, John W.

1989-01-01

443

County of Santa Clara Department of Planning and Development  

E-print Network

County of Santa Clara Department of Planning and Development County Government Center, East Wing 70 Commission From: Tom Whisler, Building Official Subject: Locally Adopted Energy Standards for Santa Clara County Today the Santa Clara County Board of Supervisors adopted Ordinance No. NS- 1100.114 modifying

444

VolusiaCounty Number of volunteers 1,363  

E-print Network

Giving Recent donors residing in county 6,578 FY 2012 donors residing in county 2,916 Gifts to UF from county residents $994,079 Gifts to IFAS from county residents $3,882 Funding State funds for Extension

Ma, Lena

445

31. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PROFILE OF HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY IN JOHNSON COUNTY, IOWA, 1897. (LEFT 3/4 OF DRAWING) - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

446

34. Photocopy of original drawing in possession of the County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

34. Photocopy of original drawing in possession of the County Auditor, Johnson County, Iowa. PLAN OF RIVER PIERS FOR HIGHWAY BRIDGE OVER CEDAR RIVER AT SUTLIFF'S FERRY, JOHNSON COUNTY, IOWA, 1897 - Sutliff's Ferry Bridge, Spanning Cedar River (Cedar Township), Solon, Johnson County, IA

447

Computer Technology in Rural Schools: The Case of Mendocino County.  

ERIC Educational Resources Information Center

The county education office of Mendocino County, California, serving nine school districts and 11,800 elementary and secondary students, began planning for computers in 1979-1980, purchased two central computers, and by 1983 had one computer or terminal for every 40 students in the county. The county was characterized by its very enthusiastic

Hoachlander, E. Gareth

448

Evaluation of the Union County Alternative to Suspension Program  

ERIC Educational Resources Information Center

The schools in Union County have undergone a tremendous amount of growth in the past decade. The growth in the county has led to an increase in discipline problems. In order to provide suspended students a second chance, Union County Public Schools implemented an alternative to suspension program, the Union County Alternative to Suspension Program

Dunlap, Joyce Ann

2010-01-01

449

Planning for Hazards and Climate Change Impacts: One County's Approach  

E-print Network

's unique topography, computer models predict that sea level rise will increase flooding on both the easternPlanning for Hazards and Climate Change Impacts: One County's Approach Miami-Dade County, Florida Like most coastal areas, Florida's Miami-Dade County is vulnerable to climate change. With the county

450

Environmental assessment, Deaf Smith County site, Texas  

SciTech Connect

The Nuclear Waste Policy Act of 1982 (42 USC sections 10101-10226) requires the environmental assessment of a proposed site to include a statement of the basis for nominating a site as suitable for characterization. Volume 2 provides a detailed statement evaluating the site suitability of the Deaf Smith County Site under DOE siting guidelines, as well as a comparison of the Deaf Smith County Site to the other sites under consideration. The evaluation of the Deaf Smith County Site is based on the impacts associated with the reference repository design, but the evaluation will not change if based on the Mission Plan repository concept. The second part of this document compares the Deaf Smith County Site to Davis Canyon, Hanford, Richton Dome and Yucca Mountain. This comparison is required under DOE guidelines and is not intended to directly support subsequent recommendation of three sites for characterization as candidate sites. 259 refs., 29 figs., 66 refs. (MHB)

Not Available

1986-05-01

451

Orange County Black History Committee Scholarship Application  

E-print Network

: ___________________________ High School GPA: __________ Are you the first in your immediate family to attend college? ______ YesOrange County Black History Committee Scholarship Application 13/14 Academic Year Name

Van Stryland, Eric

452

Charging Up in King County, Washington  

SciTech Connect

King County, Washington is spearheading a regional effort to develop a network of electric vehicle charging stations. It is also improving its vehicle fleet and made significant improvements to a low-income senior housing development.

Constantine, Dow; Oliver, LeAnn; Inslee, Jay; Sahandy, Sheida; Posthuma, Ron; Morrison, David

2011-01-01

453

County Employment Of West Virginia Higher  

E-print Network

County Employment Of West Virginia Higher Education Graduates 2009 December 2010 Prepared I: Detailed Description Of Employment Data .........................................26 Appendix II. Employment Shares And Annualized Wages Of Graduates By Region Of Employment In 2009

Mohaghegh, Shahab

454

A Seniors' Program for Beaver County  

ERIC Educational Resources Information Center

Programs for seniors are needed, according to the assistant director of continuing education at the Community College of Beaver County (Pennsylvania); the question revolves around who will pay. (Editor)

Taylor, Donald F.

1973-01-01

455

holmesCounty Number of volunteers 140  

E-print Network

and forage management, Sustainable agriculture, Volunteer management, Management of forest and wildlife% Clients adopting best practices resulting in societal, economic, or environmental benefits to community 59 IFAS alumni residing in county 28 Economic Impacts Agricultural and related industries generate 1

Ma, Lena

456

Shoreline assessment of Jefferson County, Texas  

E-print Network

Shoreline erosion is an issue of economic and environmental concern on the Texas coast. Texas State Highway 87, located in Jefferson County, Texas, has been repeatedly destroyed by storms and rebuilt in the past 50 years. Reconstruction of State...

Lee, Hoo Il

2004-09-30

457

Multipurpose Centers in a Rural County.  

ERIC Educational Resources Information Center

Describes services, programs, and funding for the various senior centers in Frankin County, New York, which has a predominately rural, low-income population. Services include bus transportation, nutrition programs, health and financial assistance, and social events. (MF)

Norris, Jean

1978-01-01

458

Wild Lake County Building Backyard Habitats  

E-print Network

species of wildlife. Alien Invaders Non-native plants and animals threaten the vitality of natural, and Coyotes, and Bears OH MY!! Humans are not the only animals who call Lake County home. Learn what to do

Florida, University of

459

Environmental assessment: Deaf Smith County site, Texas  

SciTech Connect

In February 1983, the US Department of Energy (DOE) identified a location in Deaf Smith County, Texas, as one of the nine potentially acceptable sites for mined geologic repository for spent nuclear fuel and high-level radioactive waste. To determine their suitability, the Deaf Smith County site and eight other potentially acceptable sites have been evaluated in accordance with the DOE's General Guidelines for the Recommendation of Sites for the Nuclear Waste Repositories. The Deaf Smith County site is in the Permian Basin, which is one of five distinct geohydrologic settings considered for the first repository. On the basis of the evaluations reported in this EA, the DOE has found that the Deaf Smith County site is not disqualified under the guidelines.

Not Available

1986-05-01

460

sarasotaCounty Number of volunteers 666  

E-print Network

Ension: Florida Friendly Landscaping, Master Gardener, Commercial Horticulture, Local and Sustainable Agriculture, Family and Consumer Sciences, 4-H, Community Development Giving Recent donors residing in county 5,009 FY

Ma, Lena

461

Digital geologic map of Beaver County, Oklahoma  

USGS Publications Warehouse

This data set consists of digital data and accompanying documentation for the surficial geology of Beaver County, Oklahoma. The original data are from the Hydrogeologic Map, sheet 1 of 3, included in the U.S. Geological Survey publication, Reconnaissance of the Water Resources of Beaver County, Oklahoma, Hydrologic Investigations Atlas HA-450, Morton and Goemaat, 1973. The geology was compiled by S.L. Schoff, 1953.

Cederstrand, J.R.

1997-01-01

462

Edwards Aquifer Evaluation: Kinney County, Texas  

NASA Astrophysics Data System (ADS)

The Edwards Aquifer is one of the most studied and most prolific aquifers in the United States. The aquifer is a heavily fractured and faulted carbonate aquifer with transmissivities in excess of 100 ft2/s. The City of San Antonio relies upon the Edwards Aquifer as its sole source for water. Much work has been done on quantifying recharge to the aquifer and discharge from wells and acquiring aquifer characteristics from pumping tests, specific capacity tests, and geophysical logs. Although the aquifer has been well studied in Bexar County, much less is known about the Edwards Aquifer in Kinney County. This is partly due to the lower population within the county (approximately 3,500 people) relative to the eastern counties (Uvalde, Medina, Bexar, Comal, and Hays) and the great distance of Kinney County from high profile discharge areas such as the City of San Antonio and Comal and San Marcos Springs. Three key products resulted from this study: (1) exploratory well drilling and the largest aquifer test in the county that were conducted to evaluate the well yields within a 10,000 acre study area in which a drawdown of 2.5 ft approximately 1.2 miles away was observed while pumping at approximately 4,600 gpm; (2) a recharge estimate for the Edwards Aquifer within Kinney County of approximately 71,382 ac-ft/yr; and (3) locating the Brackettville Groundwater Divide from an evaluation of ground water flow direction and hydrograph analysis. These results help evaluate the complex hydraulics occurring within Kinney County and aid in development of ground water modeling that will be used in managing the Edwards Aquifer.

Khorzad, Kaveh

2003-10-01

463

The Soils of Bowie, Denton, Freestone, and Red River Counties.  

E-print Network

leave. ***In cooperation w~th U. S. Department of Agricu ****In cooperation with the School of Agriculture SYNOPSIS This is the eleventh bulletin dealing, by counties, with the chemical composition of Texas soils, and discusses the soils of Bowie... of Freestone County 31 Alphabetical List of Soil Series of Freestone County ........ 32 ................................... Soils of Red River County 38 ................................... Chemical Composition 38 Classification of Soils of Red River County...

Fraps, G. S. (George Stronach)

1928-01-01

464

The flora of Oktibbeha County, Mississippi  

USGS Publications Warehouse

We surveyed the flora of Oktibbeha County, Mississippi, U.S.A., from February 1994 to 1996. Occupying 118 square kilometers in east-central Mississippi, Oktibbeha County lies among 3 physiographic regions that include, from west to east, Interior Flatwoods, Pontotoc Ridge, and Black Prairie. Accordingly, the county harbors a diverse flora. Based on field work, as well as an extensive review of published literature and herbarium records at IBE and MISSA, we recorded a total of 1,148 taxa (1,125 species, 7 hybrids, 16 infraspecific taxa) belonging to 514 genera in 160 families, over 85% of all taxa documented were native. Compared to 3 other counties in east-central Mississippi, Oktibbeha County has the second largest recorded flora. The number of state-listed (endangered, threatened, or of special concern) taxa (67) documented in this survey far exceeds that reported from any other county in the region. Three introduced species, Ilex cornuta Lindl. & Paxton, Mahonia bealei (Fortune) Carrie??re, and Nandina domestica Thunb., are reported in a naturalized state for the first time from Mississippi. We also describe 16 different plant communities belonging to 5 broad habitat categories: bottomland forests, upland forests and prairies, aquatic habitats, seepage areas, and human-influenced habitats. A detailed description of the vegetation associated with each of these communities is provided.

Leidolf, A.; McDaniel, S.; Nuttle, T.

2002-01-01

465

Human and Swine Hosts Share Vancomycin-Resistant Enterococcus faecium CC17 and CC5 and Enterococcus faecalis CC2 Clonal Clusters Harboring Tn1546 on Indistinguishable Plasmids?  

PubMed Central

VRE isolates from pigs (n = 29) and healthy persons (n = 12) recovered during wide surveillance studies performed in Portugal, Denmark, Spain, Switzerland, and the United States (1995 to 2008) were compared with outbreak/prevalent VRE clinical strains (n = 190; 23 countries; 1986 to 2009). Thirty clonally related Enterococcus faecium clonal complex 5 (CC5) isolates (17 sequence type 6 [ST6], 6 ST5, 5 ST185, 1 ST147, and 1 ST493) were obtained from feces of swine and healthy humans. This collection included isolates widespread among pigs of European Union (EU) countries since the mid-1990s. Each ST comprised isolates showing similar pulsed-field gel electrophoresis (PFGE) patterns (?6 bands difference; >82% similarity). Some CC5 PFGE subtype strains from swine were indistinguishable from hospital vancomycin-resistant enterococci (VRE) causing infections. A truncated variant of Tn1546 (encoding resistance to vancomycin) and tcrB (coding for resistance to copper) were consistently located on 150- to 190-kb plasmids (reppLG1). E. faecium CC17 (ST132) isolates from pig manure and two clinical samples showed identical PFGE profiles and contained a 60-kb mosaic plasmid (repInc18 plus reppRUM) carrying diverse Tn1546-IS1216 variants. The only Enterococcus faecalis isolate obtained from pigs (CC2-ST6) corresponded to a multidrug-resistant clone widely disseminated in hospitals in Italy, Portugal, and Spain, and both animal and human isolates harbored an indistinguishable 100-kb mosaic plasmid (reppRE25 plus reppCF10) containing the whole Tn1546 backbone. The results indicate a current intra- and international spread of E. faecium and E. faecalis clones and their plasmids among swine and humans. PMID:21227995

Freitas, Ana R.; Coque, Teresa M.; Novais, Carla; Hammerum, Anette M.; Lester, Camilla H.; Zervos, Marcus J.; Donabedian, Susan; Jensen, Lars B.; Francia, Maria Victoria; Baquero, Fernando; Peixe, Luisa

2011-01-01

466

Agreement between Luzerne County Community College and Luzerne County Community College Association of Higher Education.  

ERIC Educational Resources Information Center

This agreement between the Luzerne County Community College and the Luzerne County Community College Association of Higher Education covers the 1973-74 year. The agreement includes the collective bargaining agreement, definitions, recognition, fair practices, conference, maintenance of membership, check-off, grievance procedure, rights of college,

Luzerne County Community Coll., Nanticoke, PA.

467

The State of Texas Children 1994: A County by County Fact Book.  

ERIC Educational Resources Information Center

As part of a nationwide effort to highlight the well-being of children in every state, Texas Kids Count is building a comprehensive database of indicators of child well-being to be used to produce annual data books and other analyses on the status of children in Texas. This report presents, county by county, data on several child well-being

Texas Kids Count Project, Austin.

468

The Economic Impact of Johnson County Community College on Johnson County, 1999-2000.  

ERIC Educational Resources Information Center

This document is a report on the economic impact of Johnson County Community College (JCCC) (Kansas) on Johnson County and the surrounding community. The information from the economic impact study are used to support the community college's effectiveness, assessment, and accreditation efforts. Results indicate that: (1) the Community College

Johnson County Community Coll., Overland Park, KS. Office of Institutional Research.

469

Beginnings of geothermal impact on county population and leadership, Imperial County, California  

SciTech Connect

A major geothermal energy development scenario is about to begin in Imperial County. Initial energy-socioeconomic interactions in the areas of population and county leadership structure are discussed. These include immigration of energy company workers, a sewage plant dispute, conflict over union affiliation of geothermal laborers, and a transmission corridor routing dispute.

Pick, J.B.; Butler, E.W.

1980-09-01

470

The California Child Care Portfolio, 1997: A Compilation of Data about Child Care, County by County.  

ERIC Educational Resources Information Center

This compendium provides standardized data on child care supply and requests for care in California. It provides county and statewide information based on responses from more than 38,000 child care providers and about 45,000 requests for child care, including: (1) key demographic statistics; (2) county population; (3) children in poverty; (4)

California Child Care Resource and Referral Network, San Francisco.

471

BLACK-TAILED PRAIRIE DOG SURVEYS IN CROWLEY, OTERO, PUEBLO AND EASTERN HUERFANO COUNTIES, COLORADO  

E-print Network

BLACK-TAILED PRAIRIE DOG SURVEYS IN CROWLEY, OTERO, PUEBLO AND EASTERN HUERFANO COUNTIES, COLORADO............................................................................................8 Crowley County.........................................................................................8 Huerfano County

472

76 FR 30080 - Revisions to the California State Implementation Plan, Placer County Air Pollution Control...  

Federal Register 2010, 2011, 2012, 2013

...Implementation Plan, Placer County Air Pollution Control District and Ventura County Air Pollution Control District AGENCY: Environmental...revisions to the Placer County Air Pollution Control District (PCAPCD)...

2011-05-24

473

76 FR 30025 - Revisions to the California State Implementation Plan, Placer County Air Pollution Control...  

Federal Register 2010, 2011, 2012, 2013

...Implementation Plan, Placer County Air Pollution Control District and Ventura County Air Pollution Control District AGENCY: Environmental...revisions to the Placer County Air Pollution Control District (PCAPCD)...

2011-05-24

474

Estimation methods and monitoring network issues in the quantitative estimation of land-based COD and TN loads entering the sea: a case study in Qingdao City, China.  

PubMed

At present, the monitoring network of China cannot provide sufficient data to estimate land-based pollutant loads that enter the sea, and estimation methods are imprecisely used. In this study, the selection of monitoring stations, monitoring frequency, and pollutant load estimation methods was studied in Qingdao City, a typical coastal city in China, taken as an example. Land-based pollutant loads from Qingdao were estimated, and load distribution, density, and composition were analyzed to identify the key pollution source regions (SRs) that need to be monitored and controlled. Results show that the administrative land area of Qingdao can be divided into 25 sea-sink source regions (SSRs). A total of 14 more rivers and 62 industrial enterprises should be monitored to determine the comprehensive pollutant loads of the city. Furthermore, the monitoring frequency of rivers should not be less than three times/year; a monitoring frequency of five or more times is preferable. The findings on pollutant load estimation with the use of different estimation methods substantially vary; estimation results with the use of ratio-based methods were 10 and 22 % higher than those with the use of monitoring-based methods in terms of chemical oxygen demand (COD) and total nitrogen (TN), respectively. None-point sources contributed the majority of the pollutant loads at about 70 % of the total COD and 60 % of the total TN. PMID:24865505

Su, Ying; Wang, Xiulin; Li, Keqiang; Liang, Shengkang; Qian, Guodong; Jin, Hong; Dai, Aiquan

2014-09-01

475

Identifying Distal cis-acting Gene-Regulatory Sequences by Expressing BACs Functionalized with loxP-Tn10 Transposons in Zebrafish  

PubMed Central

Bacterial Artificial Chromosomes (BACs) are large pieces of DNA from the chromosomes of organisms propagated faithfully in bacteria as large extra-chromosomal plasmids. Expression of genes contained in BACs can be monitored after functionalizing the BAC DNA with reporter genes and other sequences that allow stable maintenance and propagation of the DNA in the new host organism. The DNA in BACs can be altered within its bacterial host in several ways. Here we discuss one such approach, using Tn10 mini-transposons, to introduce exogenous sequences into BACs for a variety of purposes. The largely random insertions of Tn10 transposons carrying lox sites have been used to position mammalian cell-selectable antibiotic resistance genes, enhancer-traps and inverted repeat ends of the vertebrate transposon Tol2 precisely at the ends of the genomic DNA insert in BACs. These modified BACs are suitable for expression in zebrafish or mouse, and have been used to functionally identify important long-range gene regulatory sequences in both species. Enhancer-trapping using BACs should prove uniquely useful in analyzing multiple discontinuous DNA domains that act in concert to regulate expression of a gene, and is not limited by genome accessibility issues of traditional enhancer-trapping methods. PMID:24772295

Chatterjee, Pradeep K.; Shakes, Leighcraft A.; Wolf, Hope M.; Mujalled, Mohammad A.; Zhou, Constance; Hatcher, Charles; Norford, Derek C.

2014-01-01

476

The pleistocene geology of Vilas County, Wisconsin  

NASA Astrophysics Data System (ADS)

The Wisconsin Valley, Langlade and Ontonagon Lobes of the Laurentide Ice Sheet flowed across all or part of Villas County between 23,000 and 10,000 years ago. These lobes deposited the Wildcat Lake, Nashville and Crab Lake Members of the Cooper Falls formation respectively. Each member is defined by the physical characteristics of a till, and each member also contains supraglacial debris flow sediment, and subglacial and proglacial fluvial sediment. A number of former ice margin positions are indicated in Vilas County by moraines and the ice contact slope of fluvial landforms. Regional correlation shows that the Langlade and Green Bay Lobes remained near their maximum Late Wisconsin extent when the Wisconsin Valley and Chippewa Lobes had retreated up to 100 km. The Ontonagon Lobe advanced into northern Vilas County and deposited the Winegar Moraine, probably just before growth of the Two Creeks Forest Bed in east central Wisconsin.

Attig, J. W., Jr.

1984-12-01

477

The breeding bird atlas of Montgomery and Howard Counties, Maryland  

USGS Publications Warehouse

A pilot Breeding Bird Atlas was initiated during 1971 in Montgomery County. A second atlas was begun during 1973 in Howard County. The Montgomery project was completed in 1973 and the Howard project in 1975......A total of 138 species were recorded for the two counties (129 confirmed, 7 probable, 2 possible). Montgomery County had 135 species recorded (122 confirmed, 9 probable, 4 possible) and Howard County had 127 species (110 confirmed, 7 probable,10 possible)......Thirty-two species were found in all 5-km blocks and eight were found in all 5-krn and 21/2-km blocks. Two species were confirmed in all blocks.......There were 11 new Montgomery County breeding records and eight new Howard County records. Nine species were found only in Montgomery County and two only in Howard County. Twelve species were confrmed in Montgomery County but not in Howard; five were confirmed in Howard County but not in Montgomery. Ninety-eight species were confirmed on active nests in Montgomery County and 85 in Howard County.

Klimkiewicz, M.K.; Solem, J.K.

1978-01-01

478

Dutchess County Resource Recovery Task Force report: Dutchess County Pyrolysis Program  

SciTech Connect

Dutchess County initiated development of a long-range master plan for Solid Waste Management in 1971. The plan included development of a resource recovery facility to service the municipalities in the County population center. Based on early recommendations, a pyrolysis facility employing Purox technology was to be implemented. A feasibility study, paid for by County funds was completed in 1975. The study provided siting recommendations, estimation of available waste, and preliminary facility design. Because of various considerations, the project was not developed. Under the Department of Energy grant, the County reassessed the feasibility of a resource recovery facility, with emphasis on confirming previous conclusions supporting the Purox technology, waste availability, energy recovery and sale and siting of the plant. The conclusions reached in the new study were: a resource recovery facility is feasible for the County; sufficient waste for such a facility is available and subject to control; While Purox technology was feasible it is not the most appropriate available technoloy for the County; that mass burning with steam recovery is the most appropriate technology; and that resource recovery while presently more expensive than landfilling, represents the only cost effective, energy efficient, and environmentally sound way to handle the solid waste problem in the County.

None

1980-07-01

479

Geologic Map of Loudoun County, Virginia  

USGS Publications Warehouse

Introduction The geology of Loudoun County, Va., was mapped from 1988 through 1991 under a cooperative agreement between the U.S. Geological Survey (USGS) and the Loudoun County Office of Mapping and Geographic Information. This geologic map was compiled in 1993 from a series of detailed published and unpublished field investigations at scales of 1:12,000 and 1:24,000. Some of these same data were compiled as a digital geologic map at 1:100,000 scale (Burton and others, 1992a) and were the basis for a cost-benefit analysis of the societal value of geologic maps (Bernknopf and others, 1993).

Southworth, Scott; Burton, William C.; Schindler, J. Stephen; Froelich, Albert J.

2006-01-01

480

Hampden county: a model for seamless care.  

PubMed

The Hampden County Correctional Center is helping to create a model program of correctional health care. The program objective is to provide comprehensive care that will continue seamlessly upon the release of the inmate from jail. The discharge planning process recognizes the existence of other problems such as alcoholism and substance abuse and links inmates with community-based agencies. These linkages have contributed to reduced recidivism rates. The program uses health clinic staff and public health students, rather than volunteers. The National Institute of Justice reports that the Hampden County model could be replicated in about 80 percent of corrections facilities in metropolitan areas. PMID:11366663

1999-12-24

481

Serum-Free Culture of the Suspension Cell Line QB-Tn9-4s of the Cabbage Looper, Trichoplusia ni, is Highly Productive for Virus Replication and Recombinant Protein Expression  

PubMed Central

Serum-free cultures of insect cells play an important role in the fields of protein engineering, medicine, and biology. In this paper, the suspension cell line QB-Tn9-4s of Trichoplusia ni (Hbner) (Lepidoptera: Noctuidae) was successfully adapted to serum-free Sf-900 III medium and passaged for 52 generations. The adapted QB-Tn9-4s cells grew faster. Their population doubling time shortened from 27.4 hr in serum-containing medium to 24.1 hr, and their maximal density increased by 1.83-fold, reaching 3.50 106 cells/mL in serum-free culture in T-flasks. The cells readily adapted to spinner culture, with maximum cell density of 4.40 106 cells/mL in a spinner flask. Although the infection rate of Autographa californica multiple nucleopolyhedrovirus and production of occlusion bodies (OBs) of the adapted QB-Tn9-4s cells were 91.0% and 85.4 OBs/cell, respectively, similar to those of QB-Tn9-4s cells cultured in serum-containing medium and control BTI-Tn5B1-4 cells, their budded virus titer was 4.97 107 TCID50/mL, significantly higher than those of the latter two. In addition, the expression levels of ?-galactosidase at six days post-infection and secreted alkaline phosphatase at seven days postinfection in the adapted QB-Tn9-4s cells reached 2.98 0.15104 IU/mL and 3.34 0.13 IU/mL, respectively, significantly higher than those of QB-Tn9-4s and control BTI-Tn5B1-4 cultured in serum-containing media. The above findings establish a foundation for industrial production of virus and recombinant proteins in QB-Tn9-4s serum-free culture.

Zheng, Gui-Ling; Zhou, Hong-Xu; Li, Chang-You

2014-01-01

482

5. TUNNEL TREE AT DRIVETHROUGHTREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. TUNNEL TREE AT DRIVE-THROUGH-TREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NE. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

483

76 FR 19030 - Lyon & Mineral County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013

...OF AGRICULTURE Forest Service Lyon & Mineral County Resource Advisory Committee AGENCY...SUMMARY: The Lyon and Mineral County Resource Advisory Committee will meet in Smith Valley, Nevada. The committee is meeting...

2011-04-06

484

Kalamazoo County Health Indicators Disaggregated by Race, Place and  

E-print Network

1 Kalamazoo County Health Indicators Disaggregated by Race, Place and Socioeconomic Status and Key References for Understanding Health Disparities and for Building Healthier Communities Annie Wendt Epidemiologist Kalamazoo County Department of Health and Community Services Timothy Ready Lewis Walker Institute

de Doncker, Elise

485

9. Photocopy of drawing (from York County Historical Society) William ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Photocopy of drawing (from York County Historical Society) William Wagner, artist ca. 1830 SOUTH FRONT AND WEST SIDE (TAVERN ON FAR LEFT; JOSEPH CHAMBERS HOUSE ADJOINING ON RIGHT) - Golden Plough Tavern, 159 West Market Street, York, York County, PA

486

Primary Care Development Guide for King County. Initial Report.  

National Technical Information Service (NTIS)

The improvement of primary care services rendered in private physician offices, hospital emergency rooms, and community clinics in King County, Washington is addressed. Health planning councils in four Washington counties (King, Kitsap, Pierce, and Snohom...

1975-01-01

487

75 FR 51749 - Shasta County Resource Advisory Committee  

Federal Register 2010, 2011, 2012, 2013

...Forest Service Shasta County Resource Advisory Committee AGENCY...SUMMARY: The Shasta County Resource Advisory Committee (RAC...meet RAC funding 2010/2011 allocations. DATES: Wednesday, September...FURTHER INFORMATION CONTACT: Resource Advisory Committee...

2010-08-23

488

Silver City Grant County Airport (SVC) Pavement Condition and Analysis  

E-print Network

. Recommend Pavement Design 19 ......................................................Table 5. Design Aircraft. Silver City Grant County Airport (SVC) Aircraft Operations (2008-2009) 5 .........Table 2. Silver City Grant County Airport (SVC) Predicted Aircraft Operations 2007-2027 6 ............................Figure

Cal, Mark P.

489

22. Historic American Buildings Survey Alameda County Recorder Office Map ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. Historic American Buildings Survey Alameda County Recorder Office Map Book 6, page 17 October 1960 SURVEY OF 1868 - Mission San Jose de Guadalupe, Mission & Washington Boulevards, Fremont, Alameda County, CA

490

75 FR 71179 - Environmental Impact Statement: San Diego County, CA  

Federal Register 2010, 2011, 2012, 2013

...Environmental Impact Statement: San Diego County, CA AGENCY: Federal Highway...for a proposed highway project in San Diego County, California. DATES: Public...Community Center, 2258 Island Avenue, San Diego, California 92102. FOR...

2010-11-22

491

75 FR 1051 - Christian County Generation, LLC; Notice of Filing  

Federal Register 2010, 2011, 2012, 2013

...Commission [Docket No. EL10-27-000] Christian County Generation, LLC; Notice of Filing December 30, 2009. Take notice that on December 23, 2009, Christian County Generation, LLC pursuant to section 207 of the Federal Energy...

2010-01-08

492

Small Power Plant Exemption (06-SPPE-1) Imperial County  

E-print Network

Small Power Plant Exemption (06-SPPE-1) Imperial County NILAND GAS TURBINE PLANT COMMISSIONDECISION ENERGY COMMISSION Small Power Plant Exemption (06-SPPE-1) Imperial County NILAND GAS TURBINE PLANT Materials Management........................................................... 8 Land Use, Recreation

493

75 FR 18941 - Environmental Impact Statement: Bexar County, TX  

Federal Register 2010, 2011, 2012, 2013

...updated San Antonio- Bexar County Metropolitan Planning Organization Mobility 2035 Plan (December 2009) and in response...in the San Antonio-Bexar County Metropolitan Planning Organization's Mobility 2035 Plan (adopted December...

2010-04-13

494

Perspective view of County Courthouse (513 Center Street), view looking ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Perspective view of County Courthouse (513 Center Street), view looking south - Lakeview Downtown Historic District, E, F & G Streets between Second Street North & First Street South, Lakeview, Lake County, OR

495

Regional Waterway Management System For Lee County, Phase 3.  

National Technical Information Service (NTIS)

The Regional Waterway Management System for Lee County is a collaborative effort by the Lee County Division of Natural Resources, the West Coast Inland Navigation District, and the University of Florida Sea Grant Program. The Phase 3 report addresses the ...

D. A. Fann, G. A. Antonini, L. C. Alexander, R. A. Swett

2002-01-01

496

Regional Waterway Management System for Lee County, Phase 2.  

National Technical Information Service (NTIS)

The Regional Waterway Management System for Lee County is a collaborative effort by the Lee County Division of Natural Resources, the West Coast Iniand Navigation District, and the University of Florida Sea Grant Program. The Phase 2 report addresses the ...

D. A. Fann, G. A. Antonini, L. C. Alexander, R. A. Swett

2001-01-01

497

12. Photocopy of map from Atlas of Westchester County by ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Photocopy of map from Atlas of Westchester County by Joseph E. Bien, published by Julius Bien & Company, New York, 1893 - Moquette Row Housing, Moquette Row North & Moquette Row South, Yonkers, Westchester County, NY

498

Commercial Motor Vehicle Roadside Technology Corridor (CMVRTC)  

E-print Network

managed by UT-Battelle, LLC for the U.S. Department of Energy under Contract number DE-AC05-00OR22725, and Sevier counties. It is anchored on the west end by the Knox County CMV Inspection Station (IS) and on the east end by the Greene County CMV IS. The CMVRTC is operated by the Center for Transportation Analysis

499

Yard and Home Finishing Work for Southern Lincoln County, Nevada  

Microsoft Academic Search

SUMMARY A large opportunity for business development in Lincoln County can come from the developments in southern Lincoln County. The expenditure on home and yard finishing is increasing, especially in the housing cost ranges that will be found in the new developments and with the types of individuals likely to become residents in the southern Lincoln County communities. The latest

Holly Gatzke

500

75 FR 53371 - Environmental Impact Statement; Santa Rosa County, FL  

Federal Register 2010, 2011, 2012, 2013

...Administration Environmental Impact Statement; Santa Rosa County, FL AGENCY: Federal Highway...prepared for a proposed highway project in Santa Rosa County, Florida. FOR FURTHER INFORMATION...EIS for a proposal to improve SR 87 in Santa Rosa County, Florida. The proposed...

2010-08-31