13. Roadway and place of a thousand drips looking ESE. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Roadway and place of a thousand drips looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

5. Abandoned mule trail tunnel. 1 mile from intersection with ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Abandoned mule trail tunnel. 1 mile from intersection with Newfound Gap Road looking SSE. - Great Smoky Mountains National Park Roads & Bridges, Clingmans Dome Road, Between Newfound Gap Road & Clingmans Dome, Gatlinburg, Sevier County, TN

7. Roaring Fork Motor Nature Trail, rocks along edge of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

2. Roaring Fork Motor Nature Trail, road view before first ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

17. Underside of bridge and abutment with large boulder looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. Underside of bridge and abutment with large boulder looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

6. Roaring Fork Motor Nature Trail, road view after stop ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

1. Entrance to Heintooga Round Bottom Road and sign looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Entrance to Heintooga Round Bottom Road and sign looking N. - Great Smoky Mountains National Park Roads & Bridges, Heintooga Round Bottom Road & Balsam Mountain Road, Between Blue Ridge Parkway & Big Cove Road, Gatlinburg, Sevier County, TN

3. Roaring Fork Motor Nature Trail, view between second and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

11. Roaring Fork Motor Nature Trail, boulders along road after ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

12. Roaring Fork Motor Nature Trail, place of a thousand ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

1. Opposition sign posted on private property at the entrance ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Opposition sign posted on private property at the entrance to the road looking NW. - Great Smoky Mountains National Park Roads & Bridges, Northshore Road, 1 mile spur at Fontana Dam & Bryson City to Noland Creek, Gatlinburg, Sevier County, TN

15. Culvert and corrugated pipe with place of a thousand ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. Culvert and corrugated pipe with place of a thousand drips in background looking S. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

14. Roaring Fork Motor Nature Trail, Place of a thousand ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

19. Photographer unknown, circa 1950 'THE LOOP OVER' BRIDGE WAS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. Photographer unknown, circa 1950 'THE LOOP OVER' BRIDGE WAS CONSTRUCTED ON NEWFOUND GAP ROAD TO REPLACE A SERIES OF DANGEROUS SWITCHBACKS. THIS ENGINEERING FEATURE QUICKLY BECAME A POPULAR TOURIST ATTRACTION. - Great Smoky Mountains National Park Roads & Bridges, Gatlinburg, Sevier County, TN

Late Quaternary faulting in the Sevier Desert driven by magmatism.

PubMed

Stahl, T; Niemi, N A

2017-03-14

Seismic hazard in continental rifts varies as a function of strain accommodation by tectonic or magmatic processes. The nature of faulting in the Sevier Desert, located in eastern Basin and Range of central Utah, and how this faulting relates to the Sevier Desert Detachment low-angle normal fault, have been debated for nearly four decades. Here, we show that the geodetic signal of extension across the eastern Sevier Desert is best explained by magma-assisted rifting associated with Plio-Pleistocene volcanism. GPS velocities from 14 continuous sites across the region are best-fit by interseismic strain accumulation on the southern Wasatch Fault at c. 3.4 mm yr -1 with a c. 0.5 mm yr -1 tensile dislocation opening in the eastern Sevier Desert. The characteristics of surface deformation from field surveys are consistent with dike-induced faulting and not with faults soling into an active detachment. Geologic extension rates of c. 0.6 mm yr -1 over the last c. 50 kyr in the eastern Sevier Desert are consistent with the rates estimated from the geodetic model. Together, these findings suggest that Plio-Pleistocene extension is not likely to have been accommodated by low-angle normal faulting on the Sevier Desert Detachment and is instead accomplished by strain localization in a zone of narrow, magma-assisted rifting.

Late Quaternary faulting in the Sevier Desert driven by magmatism

PubMed Central

Stahl, T.; Niemi, N. A.

2017-01-01

Seismic hazard in continental rifts varies as a function of strain accommodation by tectonic or magmatic processes. The nature of faulting in the Sevier Desert, located in eastern Basin and Range of central Utah, and how this faulting relates to the Sevier Desert Detachment low-angle normal fault, have been debated for nearly four decades. Here, we show that the geodetic signal of extension across the eastern Sevier Desert is best explained by magma-assisted rifting associated with Plio-Pleistocene volcanism. GPS velocities from 14 continuous sites across the region are best-fit by interseismic strain accumulation on the southern Wasatch Fault at c. 3.4 mm yr−1 with a c. 0.5 mm yr−1 tensile dislocation opening in the eastern Sevier Desert. The characteristics of surface deformation from field surveys are consistent with dike-induced faulting and not with faults soling into an active detachment. Geologic extension rates of c. 0.6 mm yr−1 over the last c. 50 kyr in the eastern Sevier Desert are consistent with the rates estimated from the geodetic model. Together, these findings suggest that Plio-Pleistocene extension is not likely to have been accommodated by low-angle normal faulting on the Sevier Desert Detachment and is instead accomplished by strain localization in a zone of narrow, magma-assisted rifting. PMID:28290529

Groundwater/surface-water interaction in central Sevier County, Tennessee, October 2015–2016

USGS Publications Warehouse

Carmichael, John K.; Johnson, Gregory C.

2017-12-14

The U.S. Geological Survey evaluated the interaction of groundwater and surface water in the central part of Sevier County, Tennessee, from October 2015 through October 2016. Stream base flow was surveyed in December 2015 and in July and October 2016 to evaluate losing and gaining stream reaches along three streams in the area. During a July 2016 synoptic survey, groundwater levels were measured in wells screened in the Cambrian-Ordovician aquifer to define the potentiometric surface in the area. The middle and lower reaches of the Little Pigeon River and the middle reaches of Middle Creek and the West Prong Little Pigeon River were gaining streams at base-flow conditions. The lower segments of the West Prong Little Pigeon River and Middle Creek were losing reaches under base-flow conditions, with substantial flow losses in the West Prong Little Pigeon River and complete subsurface diversion of flow in Middle Creek through a series of sinkholes that developed in the streambed and adjacent flood plain beginning in 2010. The potentiometric surface of the Cambrian-Ordovician aquifer showed depressed water levels in the area where loss of flow occurred in the lower reaches of West Prong Little Pigeon River and Middle Creek. Continuous dewatering activities at a rock quarry located in this area appear to have lowered groundwater levels by as much as 180 feet, which likely is the cause of flow losses observed in the two streams, and a contributing factor to the development of sinkholes at Middle Creek near Collier Drive.

75 FR 20774 - Establishment of Class E Airspace; Mountain City, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-21

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 71 [Docket No. FAA-2009-0061; Airspace Docket No. 09-ASO-10] Establishment of Class E Airspace; Mountain City, TN AGENCY... December 7, 2009 that establishes Class E airspace at Johnson County Airport, Mountain City, TN. DATES...

9. Photocopy of photograph showing myrtlecovered spring. The original photograph ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Photocopy of photograph showing myrtle-covered spring. The original photograph was taken on May 21, 1936 by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is II-B-(7)-8096. - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

Anatexis and metamorphism in tectonically thickened continental crust exemplified by the Sevier hinterland, western North America

NASA Technical Reports Server (NTRS)

Patino Douce, Alberto E.; Humphreys, Eugene D.; Johnston, A. Dana

1990-01-01

This paper presents a thermal and petrologic model of anatexis and metamorphism in regions of crustal thickening exemplified by the Sevier hinterland in western North America, and uses the model to examine the geological and physical processes leading to crustally derived magmatism. The results of numerical experiments show that anatexis was an inevitable end-product of Barrovian metamorphism in the thickened crust of the late Mesozoic Sevier orogenic belt and that the advection of heat across the lithosphere, in the form of mantle-derived mafic magmas, was not required for melting of metasedimentary rocks. It is suggested that, in the Sevier belt, as in other intracontinental orogenic belts, anatexis occurred in the midcrust and not at the base of the crust.

76 FR 4147 - Putnam-Cumberland, TN-Improve Power Supply

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

... TENNESSEE VALLEY AUTHORITY Putnam-Cumberland, TN--Improve Power Supply AGENCY: Tennessee Valley... proposed electrical power supply improvements in the Putnam and Cumberland region of east-central Tennessee... supplies bulk electric power to Cumberland and Putnam counties and the immediately surrounding areas in...

10. Photocopy of photograph showing the three Walker sisters ginning ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Photocopy of photograph showing the three Walker sisters ginning cotton. Misses Hettie, Martha and Louisa are from left to right. The original photograph was taken on May 21, 1936 by Edouard E. Exline and is one of five photographs in the album, 'A Sketch of Mountain Life: Great Smoky Mountains National Park', compiled by Edouard E. Exline and C.S. Grossman. The album is on file at the Great Smoky Mountains National Park; the photograph number is III-A-HSE-9642. - Walker Family Farm (General views), Gatlinburg, Sevier County, TN

Increasing influence of exotic terranes as sources of shales from the Sevier and Taconic Foreland basins : Evidence from Nd isotopes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Samson, S.D.; Andersen, C.B.

1994-03-01

The influence of outboard tectonostratigraphic terranes as a source of sediment to Ordovician foreland basins is unknown. To determine if there were changes in provenance, or changes in the importance of a given source region, the authors have analyzed shales from two foreland basins, the Tactonic Foreland basin of central New York and the Sevier Foreland basin of Tennessee, for their Nd isotopic compositions. Shales from the Taconic basin include those from the lower portion of Utica shale, Corynoides americanus graptolite Zone, and the uppermost portion of the Utica shale, including the Geniculograptus pygmaeus graptolite Zone. Initial [epsilon][sub Nd] valuesmore » for the oldest Taconic basin shales are [minus]12. Initial [epsilon][sub Nd] values for the younger Taconic basin shales range from [minus]9.7 to [minus]8.4. This increase in [epsilon][sub Nd] may reflect an increased influence of terranes outboard of the Laurentian margin. Samples from the Sevier basin include those from the Blockhouse and Tellico Formations. A sample of the lower Blockhouse Fm. has an initial [epsilon][sub Nd] of [minus]9.4, while mid-formation levels have [epsilon][sub Nd] = [minus]8.8. Initial [epsilon][sub Nd] ranges from [minus]8.0 to [minus]7.2 from Tellico Formation shales. Thus a trend towards increasing [epsilon][sub Nd] with decreasing age is also seen in the Sevier basin. This again suggests the possibility of an increasing influence from nearby terranes. The fact that the [epsilon][sub Nd] values are higher in the Sevier basin than in the Taconic basin indicates that the Sevier shales received detritus with a less evolved isotopic composition. This may reflect fundamentally different sources, such as a more juvenile terrane as an important source of Sevier basin shales.« less

MX Siting Investigation. Gravity Survey - Sevier Desert Valley, Utah.

DTIC Science & Technology

1981-01-24

Cheyenne, Wyoming. DMAHTC reduces the data to Simple Bouguer Anomaly (see Section A1.4, Appendix Al.0). The Defense Mapping Agency Aerospace Center...Desert Valley, Utah ......... 2 2 Topographic Setting - Sevier Desert Valley, Utah . 3 LIST OF DRAWINGS Drawing Number 1 Complete Bouguer Anomaly...gravity stations were distributed throughout the valley at an approxi- mate interval of 1.4 miles (2.3 km). Drawing 1 is a Complete Bouguer Anomaly

Ground-water data, Sevier Desert, Utah

USGS Publications Warehouse

Mower, Reed W.; Feltis, Richard D.

1964-01-01

This report is intended to serve two purposes: (1) to make available to the public basic ground-water data useful in planning and studying development of water resources, and (2) to supplement an interpretive report that will be published later.Records were collected during the period 1935-64 by the U.S. Geological survey in cooperation with the Utah State Engineer as part of the investigation of ground-water conditions in the Sevier Desert, in Juab and Millard Counties, Utah. The interpretive material will be published in a companion report by R. W. Mower and R. D. Feltis.This report is most useful in predicting conditions likely to be found in areas that are being considered as well sites. The person considering the new well can spot the proposed site on plate 1 and examine the records of nearby wells as shown in the tables and figures. From table 1 he can note such things as depth, diameter, water level, yield, use of water, temperature of water, and depth of perforations. By comparing the depth of perforations with the drillers' logs in table 3 he can note the type of material that yields water to the wells. Table 2 and figure 2 show the historic fluctuations and trends of water levels in the vicinity. From table 4 he can note the chemical quality of the water from wells in the vicinity. Table 5 shows the amount of water discharged during 1951-63 from the pumped irrigation, public supply, and industrial wells. If the reader decides from his examination that conditions are favorable, he can place an application to drill a well with the state Engineer. If the State Engineer believes unappropriated water is available, the application may be approved after minimum statutory requirements have been satisfied.The report is also useful when planning large-scale developments of water supply. This and other uses of the report will be helped by use of the interpretive report upon its release.

Quality of surface water in the Sevier Lake basin, Utah

USGS Publications Warehouse

Hahl, D.C.; Cabell, R.E.

1965-01-01

Few data are available on the quality of surface waters in the Sevier Lake basin. Because of the need for information not only on the chemical-quality but also on the other water-quality characteristics of the basin, the U.S. Geological Survey, as part of its cooperative program with the Utah State Engineer, evaluated the available data in 1963. Based on this evaluation, a reconnaissance was designed to obtain some of the needed water-quality information. To extend the applicability of the basic information, the Utah State Engineer, the Utah State Department of Health, the Water Commissioner for the Sevier River, and the Soil Conservation Service and Forest Service of the U.S. Department of Agriculture assisted in the planning and in the selection of sampling sites.This report presents the results of the data-collection phase of the reconnaissance. A companion interpretive report will be prepared later. The data were collected primarily by the U.S. Geological Survey as part of its cooperative programs with the State Engineer of Utah and the Utah Geological and Mineralogical Survey. The work was under the supervision of R. H. Langford, district chemist of the Quality of Water Branch, Water Resources Division, U.S. Geological Survey.

Structural styles of the Guess Creek fault block beneath the Great Smoky thrust sheet, Blount County, Tennessee

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carter, M.W.; Davidson, G.L.; Heller, J.A.

1993-03-01

A road cut along US 321 N, approximately 1 km NW of Walland, TN, exposes a previously unexposed complexly deformed section of Middle Ordovician clastic wedge [Chickamauga Group, Sevier Shale] sedimentary rocks. It provides an excellent opportunity to analyze both the lithologic assemblages and complex folding and faulting beneath the Great Smoky thrust sheet. Arkosic quartzite of the Lower Cambrian Cochran Conglomerate [Chilhowee Group], has been thrust over weaker Sevier Shale in the hanging wall of the Guess Creek fault. Regionally, the Great Smoky fault separates metamorphosed Precambrian to Lower Cambrian clastic shelf, slope, and rift facies rocks of themore » western Blue Ridge from Cambro-Ordovician carbonate shelf and orogenic wedge deposits of the foreland fold and thrust belt. West of the Great Smoky fault, the Guess Creek fault has been interpreted to floor duplexed Cambro-Ordovician rocks exposed in windows beneath the Great Smoky thrust sheet in the vicinity of the Great Smoky Mountains National Park. The Sevier Shale here consists of variably cleaved shale, siltstone, sandstone, and conglomerate. It exhibits a variety of fold styles throughout the exposure, ranging from predominantly noncylindrical tight folds to broad, open structures. A weak axial-planar pencil cleavage is developed in the Middle Ordovician shale and siltstone, along with a secondary cleavage that transects the axial surfaces of the folds. Minor thrust faults within the Sevier Shale appear to have formed by propagation through tightened fold hinges or bedding-parallel slip. The fold pattern observed in the roadcut appears to be partly the result of movement along a tear fault that broke both the hanging wall and footwall of the Great Smoky thrust sheet after emplacement. Slickenline orientations along minor thrust surfaces in the Cochran Conglomerate indicate eastward-directed, oblique-slip movement of the tear fault.« less

Molecular dynamics studies on troponin (TnI-TnT-TnC) complexes: insight into the regulation of muscle contraction.

PubMed

Varughese, Jayson F; Chalovich, Joseph M; Li, Yumin

2010-10-01

Mutations of any subunit of the troponin complex may lead to serious disorders. Rational approaches to managing these disorders require knowledge of the complex interactions among the three subunits that are required for proper function. Molecular dynamics (MD) simulations were performed for both skeletal (sTn) and cardiac (cTn) troponin. The interactions and correlated motions among the three components of the troponin complex were analyzed using both Molecular Mechanics-Generalized Born Surface Area (MMGBSA) and cross-correlation techniques. The TnTH2 helix was strongly positively correlated with the two long helices of TnI. The C domain of TnC was positively correlated with TnI and TnT. The N domain of TnC was negatively correlated with TnI and TnT in cTn, but not in sTn. The two C-domain calcium-binding sites of TnC were dynamically correlated. The two regulatory N-domain calcium-binding sites of TnC were dynamically correlated, even though the calcium-binding site I is dysfunctional. The strong interaction residue pairs and the strong dynamically correlated residues pairs among the three components of troponin complexes were identified. These correlated motions are consistent with the idea that there is a high degree of cooperativity among the components of the regulatory complex in response to Ca(2+) and other effectors. This approach may give insight into the mechanism by which mutations of troponin cause disease. It is interesting that some observed disease causing mutations fall within regions of troponin that are strongly correlated or interacted.

An appraisal of the quality of surface water in the Sevier Lake basin, Utah, 1964

USGS Publications Warehouse

Hahl, D.C.; Mundorff, J.C.

1968-01-01

The Sevier and Beaver River systems are the two major river systems in the Sevier Lake basin in Utah. This report contains an analysis of reconnaissance data collected during the 1964 water year regarding the quality of water in these rivers and their tributaries. The purpose of the reconnaissance was to obtain needed water-quality information for the basin. Corollary purposes were to (1) determine the suitability of surface water for specificuses, (2) determine the need and criteria for a water-quality network, and (3) locate sources of organic pollution to the rivers. Data concerning item 3 are mentioned only briefly in this report and will be discussed in a report to be prepared by the Utah Water Pollution and Control Board. Data collected in connection with the reconnaissance and resulting analyses were reported by Hahl and Cabell (1965).

78 FR 58489 - Proposed Amendment of Class E Airspace; McMinnville, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-24

... County Memorial Airport, TN (Lat. 35[deg]41'55'' N., long. 85[deg]50'38'' W.) Columbia River Park... Flight Rules (IFR) operations at the airport. DATES: Comments must be received on or before November 8, 2013. ADDRESSES: Send comments on this rule to: U. S. Department of Transportation, Docket Operations...

Ground-water conditions and geologic reconnaissance of the Upper Sevier River basin, Utah

USGS Publications Warehouse

Carpenter, Carl H.; Robinson, Gerald B.; Bjorklund, Louis Jay

1967-01-01

The upper Sevier River basin is in south-central Utah and includes an area of about 2,400 .square miles of high plateaus and valleys. It comprises the entire Sevier River drainage basin above Kingston, including the East Fork Sevier River and its tributaries. The basin was investigated to determine general ground-water conditions, the interrelation of ground water and surface water, the effects of increasing the pumping of ground water, and the amount of ground water in storage.The basin includes four main valleys - Panguitch Valley, Circle Valley, East Fork Valley, and Grass Valley - which are drained by the Sevier River, the East Fork Sevier River, and Otter Creek. The plateaus surrounding the valleys consist of sedimentary and igneous rocks that range in age from Triassic to Quaternary. The valley fill, which is predominantly alluvial gravel, sand, silt, and clay, has a maximum thickness of more than 800 feet.The four main valleys constitute separate ground-water basins. East Fork Valley basin is divided into Emery Valley, Johns Valley, and Antimony subbasins, and Grass Valley basin is divided into Koosharem and Angle subbasins. Ground water occurs under both artesian and water-table conditions in all the basins and subbasins except Johns Valley, Emery Valley, and Angle subbasins, where water is only under water-table conditions. The water is under artesian pressure in beds of gravel and sand confined by overlying beds of silt and clay in the downstream parts of Panguitch Valley basin, Circle Valley basin, and Antimony subbasin, and in most of Koosharem subbasin. Along the sides and upstream ends of these basins, water is usually under water-table conditions.About 1 million acre-feet of ground water that is readily available to wells is stored in the gravel and sand of the upper 200 feet of saturated valley fill. About 570,000 acre-feet is stored in Panguitch Valley basin, about 210,000 in Circle Valley basin, about 6,000 in Emery Valley subbasin, about 90

Genetic and molecular studies of a composite chromosomal element (Tn3705) containing a Tn916-modified structure (Tn3704) in Streptococcus anginosus F22.

PubMed

Clermont, D; Horaud, T

1994-01-01

The plasmid-free Streptococcus anginosus F22 contained a conjugative element, Tn3705, encoding resistance to erythromycin (Emr) and tetracycline-minocycline (Tcr-Mnr). We mapped a chromosomal region (> 52 kb) of F22, corresponding to the internal part of Tn3705. Molecular analysis of Tn3705 revealed it to be a composite structure: it included in its central part a transposon designated Tn3704 (20.3 kb +/- 0.5 kb), which had a modified structure in comparison with that of Tn916 and on which the Emr Tcr-Mn4 markers were localized. Tn3705 inserted from F22 into the chromosome of various streptococcal transconjugants as well as that of Enterococcus faecalis transconjugants without changing its structure. In contrast, from the chromosome of an E. faecalis::Tn3705 transconjugant only Tn3704 inserted, at various sites, into another E. faecalis chromosome. Sugar fermentations occurred after the insertion of Tn3704 into the chromosome of an asaccharolytic E. faecalis strain. Transposition of only Tn3704 from the chromosome of E. faecalis::Tn3705 onto pIP964, an E. faecalis hemolysin plasmid, yielded two different pIP964 derivatives. The size of the entire element Tn3705 was estimated to be about 70.0 kb by pulsed-field electrophoresis.

Inventory of wetlands and agricultural land cover in the upper Sevier River Basin, Utah

NASA Technical Reports Server (NTRS)

Jaynes, R. A.; Clark, L. D., Jr.; Landgraf, K. F. (Principal Investigator)

1981-01-01

The use of color infrared aerial photography in the mapping of agricultural land use and wetlands in the Sevier River Basin of south central utah is described. The efficiency and cost effectiveness of utilizing LANDSAT multispectral scanner digital data to augment photographic interpretations are discussed. Transparent overlays for 27 quadrangles showing delineations of wetlands and agricultural land cover were produced. A table summarizing the acreage represented by each class on each quadrangle overlay is provided.

Ground-water resources of the Sevier River basin between Yuba Dam and Leamington Canyon, Utah

USGS Publications Warehouse

Bjorklund, Louis Jay; Robinson, Gerald B.

1968-01-01

The area investigated is a segment of the Sevier River basin, Utah, comprising about 900 square miles and including a 19-mile reach of the Sevier River between Yuba Dam and Leamington Canyon. The larger valleys in the area are southern Juab, Round, and Scipio Valleys. The smaller valleys are Mills, Little, Dog, and Tinctic Wash Valleys.The geology of parts of Scipio, Little, and Mills Valleys and parts of the surrounding highlands was mapped and studied to explain the occurrence of numerous sinkholes in the thre valleys and to show their relation to the large springs in Mills Valley. The sinkholes, which are formed in the alluvium, are alined along faults, which penetrate both the alluvium and the underlying bedrock, and they have been formed by collapse of solution cavities in the underlying bedrock. The bedrock is mostly sandy limestone beds of the upper part of the North Horn Formation and of the Flagstaff Limestone. The numerous faults traversing Scipio Valley in a north-northeasterly direction trend directly toward Molter and Blue Springs in Mills Valley. One fault, which can be traced directly between the springs, probably is the principal channelway for the ground water moving from Scipio and Little Valleys to the springs.

[Construction of cTnC-linker-TnI (P) Genes, Expression of Fusion Protein and Preparation of Lyophilized Protein].

PubMed

Song, Xiaoli; Liu, Xiaoyun; Cai, Lei; Wu, Jianwei; Wang, Jihua

2015-12-01

In order to construct and express human cardiac troponin C-linker-troponin I(P) [ cTnC-linker-TnI(P)] fusion protein, detect its activity and prepare lyophilized protein, we searched the CDs of human cTnC and cTnI from GenBank, synthesized cTnC and cTnI(30-110aa) into cloning vector by a short DNA sequence coding for 15 neutral amino acid residues. pCold I-cTnC-linker-TnI(P) was constructed and transformed into E. coli BL21(DE3). Then, cTnC-linker-TnI(P) fusion protein was induced by isopropyl-β-D-thiogalactopyranoside (IPTG). Soluable expression of cTnC-linker-TnI(P) in prokaryotic system was successfully obtained. The fusion protein was purified by Ni²⁺ Sepharose 6 Fast Flow affinity chromatography with over 95% purity and prepared into lyophilized protein. The activity of purified cTnC-linker-TnI(P) and its lyophilized protein were detected by Wondfo Finecare™ cTnI Test. Lyophilized protein of cTnC-linker-TnI(P) was stable for 10 or more days at 37 °C and 4 or more months at 25 °C and 4 °C. The expression system established in this research is feasible and efficient. Lyophilized protein is stable enough to be provided as biological raw materials for further research.

Challenges in Antibody Development against Tn and Sialyl-Tn Antigens

PubMed Central

Loureiro, Liliana R.; Carrascal, Mylène A.; Barbas, Ana; Ramalho, José S.; Novo, Carlos; Delannoy, Philippe; Videira, Paula A.

2015-01-01

The carbohydrate antigens Tn and sialyl-Tn (STn) are expressed in most carcinomas and usually absent in healthy tissues. These antigens have been correlated with cancer progression and poor prognosis, and associated with immunosuppressive microenvironment. Presently they are used in clinical trials as therapeutic vaccination, but with limited success due to their low immunogenicity. Alternatively, anti-Tn and/or STn antibodies may be used to harness the immune system against tumor cells. Whilst the development of antibodies against these antigens had a boost two decades ago for diagnostic use, so far no such antibody entered into clinical trials. Possible limitations are the low specificity and efficiency of existing antibodies and that novel antibodies are still necessary. The vast array of methodologies available today will allow rapid antibody development and novel formats. Following the advent of hybridoma technology, the immortalization of human B cells became a methodology to obtain human monoclonal antibodies with better specificity. Advances in molecular biology including phage display technology for high throughput screening, transgenic mice and more recently molecularly engineered antibodies enhanced the field of antibody production. The development of novel antibodies against Tn and STn taking advantage of innovative technologies and engineering techniques may result in innovative therapeutic antibodies for cancer treatment. PMID:26270678

Ground-water hydrology of the upper Sevier River Basin, south-central Utah, and simulation of ground-water flow in the valley-fill in Panguitch Valley.

USGS Publications Warehouse

Thiros, Susan A.; Brothers, William C.

1993-01-01

The ground-water hydrology of the upper Sevier River basin, primarily of the unconsolidated valley-fill aquifers, was studied from 1988 to 1989. Recharge to the valley-fill aquifers is mostly by seepage from surface-water sources. Changes in soil-moisture content am water levels were measured in Panguitch Valley both at a flood-irrigated and at a sprinkler-irrigated alfalfa field to quantify seepage from unconsumed irrigation water. Lag time between irrigation and water-level response decreased from 6 to 2 days in the flood-irrigated field as the soil-moisture content increased. Water levels measured in the sprinkler-irrigated field did not respond to irrigation. Discharge from the valley-fill aquifer to the Sevier River in Panguitch Valley is about 53,570 acre-feet per year.Water levels measured in wells from 1951 to 1989 tend to fluctuate with the quantity of precipitation falling at higher elevations. Ground-water discharge to the Sevier River in Panguitch Valley causes a general increase in the specific conductance of the river in a downstream direction.A three-layered ground-water-flow model was used to simulate the effects of changes in irrigation practices am increased ground-water withdrawals in Panguitch Valley. The establishment of initial conditions consisted of comparing simulated water levels and simulated gains and losses from the Sevier River and selected canals with values measured during the 1988 irrigation season. The model was calibrated by comparing water-level changes measured from 1961 to 1963 to simulated changes. A simulated change from flood to sprinkler irrigation resulted in a maximum decline in water level of 0.9 feet after the first year of change. Simulating additional discharge from wells resulted in drawdowns of about 20 feet after the first year of pumping.

Tn5Prime, a Tn5 based 5' capture method for single cell RNA-seq.

PubMed

Cole, Charles; Byrne, Ashley; Beaudin, Anna E; Forsberg, E Camilla; Vollmers, Christopher

2018-06-01

RNA-sequencing (RNA-seq) is a powerful technique to investigate and quantify entire transcriptomes. Recent advances in the field have made it possible to explore the transcriptomes of single cells. However, most widely used RNA-seq protocols fail to provide crucial information regarding transcription start sites. Here we present a protocol, Tn5Prime, that takes advantage of the Tn5 transposase-based Smart-seq2 protocol to create RNA-seq libraries that capture the 5' end of transcripts. The Tn5Prime method dramatically streamlines the 5' capture process and is both cost effective and reliable. By applying Tn5Prime to bulk RNA and single cell samples, we were able to define transcription start sites as well as quantify transcriptomes at high accuracy and reproducibility. Additionally, similar to 3' end-based high-throughput methods like Drop-seq and 10× Genomics Chromium, the 5' capture Tn5Prime method allows the introduction of cellular identifiers during reverse transcription, simplifying the analysis of large numbers of single cells. In contrast to 3' end-based methods, Tn5Prime also enables the assembly of the variable 5' ends of the antibody sequences present in single B-cell data. Therefore, Tn5Prime presents a robust tool for both basic and applied research into the adaptive immune system and beyond.

Summary of reported agriculture and irrigation water use in southwestern Arkansas counties, 1991

USGS Publications Warehouse

Holland, T.W.; Manning, C.A.; Stafford, K.L.

1993-01-01

This report summarizes the 1991 water-use reporting through the Conservation District Offices in the following southwestern Arkansas counties: Bradley, Calhoun, Clark, Cleveland, Columbia, Dallas, Garland, Grant, Hempstead, Hot Spring, Howard, Little River, Montgomery, Nevada, Ouachita, Pike, Polk, Saline, Sevier, and Union. The number of withdrawal registrations for southwestern Arkansas counties was 132 (31 groundwater and 101 surface water). Water withdrawals reported during the registration process total 0.84 Mgal/d (none from groundwater and 0.84 Mgal/d surface water) for agriculture and 14.22 Mgal/d (1.64 Mgal/d groundwater and 12.58 Mgal/d surface water) for irrigation. The registration reports for 1991 indicate that this water was applied to 8,455 acres of land to irrigate rice, corn, sorghum, soybeans, cotton, cash grains, vegetables, sod, berries, fruit trees, timber, shrubs, and nuts as well as for the agricultural use of animal aquaculture.

Origin of the Uinta recess, Sevier fold thrust belt, Utah: influence of basin architecture on fold thrust belt geometry

NASA Astrophysics Data System (ADS)

Paulsen, Timothy; Marshak, Stephen

1999-11-01

Structural trends in the Sevier fold-thrust belt define a pronounced concave-to-the-foreland map-view curve, the Uinta recess, in north-central Utah. This recess separates two convex-to-the-foreland curves, the Provo salient on the south and the Wyoming salient on the north. The two limbs of the recess comprise transverse zones (fault zones at a high-angle to the regional trend of the orogen) that border the flanks of the east-west-trending Uinta/Cottonwood arch. Our structural analysis indicates that the transverse zones formed during the Sevier orogeny, and that they differ markedly from each other in structural style. The Charleston transverse zone (CTZ), on the south side of the arch, initiated as a complex sinistral strike-slip fault system that defines the abrupt northern boundary of the Provo salient. The Mount Raymond transverse zone (MRTZ), on the north side of the arch, represents the region in which the southeast-verging southern limb of the gently curving Wyoming salient was tilted northwards during the Laramide phase of uplift of the Uinta/Cottonwood arch. In effect, the MRTZ represents an oblique cross section through a thrust belt. The contrasting architecture of these transverse zones demonstrates how pre-deformation basin geometry influences the geometry of a fold-thrust belt. Analysis of isopach maps indicates that, at the time the Sevier fold-thrust belt formed, the area just north of the present site of the Uinta/Cottonwood arch was a basement high, with a gently dipping north flank, and a steeply dipping south flank. Thus, predeformational sediment thickened abruptly to the south of the high and thickened gradually to the north of the high. As illustrated by sandbox models, the distance that a fold-thrust belt propagates into the foreland depends on the thickness of the sedimentary layer being deformed, so the shape of the salient mimics the longitudinal cross-sectional shape of the sedimentary basin. Where basins taper gradually along strike

Stratigraphy and structure of the Sevier thrust belt and proximal foreland-basin system in central Utah: A transect from the Sevier Desert to the Wasatch Plateau

USGS Publications Warehouse

Lawton, T.F.; Sprinkel, D.A.; Decelles, P.G.; Mitra, G.; Sussman, A.J.; Weiss, M.P.

1997-01-01

The Sevier orogenic belt in central Utah comprises four north-northwest trending thrust plates and two structural culminations that record crustal shortening and uplift in late Mesozoic and early Tertiary time. Synorogenic clastic rocks, mostly conglomerate and sandstone, exposed within the thrust belt were deposited in wedge-top and foredeep depozones within the proximal part of the foreland-basin system. The geologic relations preserved between thrust structures and synorogenic deposits demonstrate a foreland-breaking sequence of thrust deformation that was modified by minor out-of-sequence thrust displacement. Structural culminations in the interior part of the thrust belt deformed and uplifted some of the thrust sheets following their emplacement. Strata in the foreland basin indicate that the thrust sheets of central Utah were emplaced between latest Jurassic and Eocene time. The oldest strata of the foredeep depozone (Cedar Mountain Formation) are Neocomian and were derived from the hanging wall of the Canyon Range thrust. The foredeep depozone subsided most rapidly during Albian through Santonian or early Campanian time and accumulated about 2.5 km of conglomeratic strata (Indianola Group). The overlying North Horn Formation accumulated in a wedge-top basin from the Campanian to the Eocene and records propagation of the Gunnison thrust beneath the former foredeep. The Canyon Range Conglomerate of the Canyon Mountains, equivalent to the Indianola Group and the North Horn Formation, was deposited exclusively in a wedge-top setting on the Canyon Range and Pavant thrust sheets. This field trip, a three day, west-to-east traverse of the Sevier orogenic belt in central Utah, visits localities where timing of thrust structures is demonstrated by geometry of cross-cutting relations, growth strata associated with faults and folds, or deformation of foredeep deposits. Stops in the Canyon Mountains emphasize geometry of late structural culminations and relationships of

Fault Dating in the US Rockies and Large Regional Extent of Deformation Pulses Along the Sevier Orogen of North America.

NASA Astrophysics Data System (ADS)

van der Pluijm, B.; Lynch, E. A.; Pana, D.; Yonkee, A.

2017-12-01

Recent Ar dating of clay-rich fault rock in the Canadian Rockies identified multiple orogenic pulses: Late Jurassic (163-146 Ma), Mid-Cretaceous (103-99 Ma), Late Cretaceous (76-72 Ma) and Eocene (54-52 Ma; Pana and van der Pluijm, GSAB 2015). New dating in the US Rockies combined with ages in the most frontal section along an Idaho-Wyoming transect show a remarkably similar age pattern: Meade Thrust, 108-102 Ma; (S)Absaroka Thrust, 73 Ma; Darby-Bear Thrust, 56-50 Ma. These radiometric fault ages in the US Rockies match field and tectono-stratigraphic predictions, analogues to those in the Canadian Rockies. Thus, a remarkably long (>1500km) lateral tract along the North American Sevier orogen is characterized by at least three major orogenic pulses that are structurally contiguous. These orogenic pulses are progressively younger in the direction of easterly thrust fault motion (toward cratonic interior) and are separated by long periods of relative tectonic quiescence. We interpret the extensive regional continuity of deformation pulses and tectonic quiescence along the Sevier Orogen as the result of three plate reorganization events in western North America since the Late Jurassic.

75 FR 430 - Notice of Intent To Prepare an Environmental Impact Statement for the Sigurd-Red Butte...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-05

...; UTU-83067] Notice of Intent To Prepare an Environmental Impact Statement for the Sigurd-Red Butte...) Cedar City Field Office, Cedar City, Utah, intends to prepare an Environmental Impact Statement (EIS... Fishlake National Forests), State of Utah, Millard County, Sevier County, Beaver County, Utah Division of...

Geologic Map of the Pahranagat Range 30' x 60' Quadrangle, Lincoln and Nye Counties, Nevada

USGS Publications Warehouse

Jayko, A.S.

2007-01-01

Introduction The Pahranagat Range 30' x 60' quadrangle lies within an arid, sparsely populated part of Lincoln and Nye Counties, southeastern Nevada. Much of the area is public land that includes the Desert National Wildlife Range, the Pahranagat National Wildlife Refuge, and the Nellis Air Force Base. The topography, typical of much of the Basin and Range Province, consists of north-south-trending ranges and intervening broad alluvial valleys. Elevations range from about 1,000 to 2,900 m. At the regional scale, the Pahranagat Range quadrangle lies within the Mesozoic and early Tertiary Sevier Fold-and-Thrust Belt and the Cenozoic Basin and Range Province. The quadrangle is underlain by a Proterozoic to Permian miogeoclinal section, a nonmarine clastic and volcanic section of middle Oligocene or older to late Miocene age, and alluvial deposits of late Cenozoic age. The structural features that are exposed reflect relatively shallow crustal deformation. Mesozoic deformation is dominated by thrust faults and asymmetric or open folds. Cenozoic deformation is dominated by faults that dip more than 45i and dominostyle tilted blocks. At least three major tectonic events have affected the area: Mesozoic (Sevier) folding and thrust faulting, pre-middle Oligocene extensional deformation, and late Cenozoic (mainly late Miocene to Holocene) extensional deformation. Continued tectonic activity is expressed in the Pahranagat Range area by seismicity and faults having scarps that cut alluvial deposits.

Superfund Record of Decision (EPA Region 4): Milan Army Ammunition Plant, Milan, TN, March 11, 1998

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1998-09-01

This decision document presents the selected action for the Salvage Yard, Former Ammunition Burnout Area (ABA), and Sanitary Landfill at MLAAP, located in Gibson and Carroll Counties, TN. This ROD addresses the final response action planned for the Salvage Yard, Former ABA, and Sanitary Landfill, including soil and groundwater. NFA is the selected remedy for soil and groundwater at the Salvage Yard, Former ABA, and Sanitary landfill. The selected remedy manages the risk to acceptable levels for both human health and the environment and is the final action planned.

Tn552 transposase purification and in vitro activities.

PubMed Central

Rowland, S J; Sherratt, D J; Stark, W M; Boocock, M R

1995-01-01

The Staphylococcus aureus transposon Tn552 encodes a protein (p480) containing the 'D,D(35)E' motif common to retroviral integrases and the transposases of a number of bacterial elements, including phage Mu, the integron-containing element Tn5090, Tn7 and IS3. p480 and a histidine-tagged derivative were overexpressed in Escherichia coli and purified by methods involving denaturation and renaturation. DNase I footprinting and gel binding assays demonstrated that p480 binds to two adjacent, directly repeated 23 bp motifs at each end of Tn552. Although donor strand cleavage by p480 was not detected, in vitro conditions were defined for strand transfer activity with transposon end fragments having pre-cleaved 3' termini. Strand transfer was Mn(2+)-dependent and appeared to join a single left or right end fragment to target DNA. The importance of the terminal dinucleotide CA-3' was demonstrated by mutation. The in vitro activities of p480 are consistent with its proposed function as the Tn552 transposase. Images PMID:7828593

Rapid Intradeformational Emplacement of the Big Hole Canyon Pluton Into the Sevier Fold-Thrust Belt, Southwest Montana.

NASA Astrophysics Data System (ADS)

Hespenheide, M. A.

2002-12-01

The Big Hole Canyon pluton (BHCp) is a Late Cretaceous pluton emplaced within the Sevier fold-and-thrust belt of the western North American Cordillera. The pluton is exposed over 60km2 and a thickness of ~1400m. Combined anisotropy of magnetic susceptibility (AMS), structural, and field studies document a clear pattern of magmatic flow radiating from at least three subvertical conduits <100m wide and ~300 to ~800m long. Interpreted flow plunges change rapidly to subhorizontal fabrics across the rest of the pluton, matching the expected pattern for laccolithic emplacement. Ascent conduits within the Big Hole Canyon pluton are coincident with the fold axis of an anticline above a thrust ramp, suggesting that the magma ascended up the fault of the fault-bend-fold. Geobarometry and stratigraphic reconstructions indicate an emplacement depth of approximately ~3km. Preliminary thermal modeling indicates that the BHCp was emplaced in 250,000 years, likely between periods of regional shortening deformation. Rapid magma ascent rates calculated by dike flow modeling and implied by entrained wall-rock xenoliths may indicate sequential magma injection into the pluton; an absence of chill margins between phases within the pluton indicates that sequential injections must have taken place quickly enough that the magmas did not have time to cool below the solidus temperature. The geometry and location of the BHCp suggest that magma used a pre-existing fault as a mechanical discontinuity for both ascent and emplacement. Continued intrusion of magma had a sufficient amount of driving pressure to stretch, shear, and lift the roof of the pluton. Detailed field mapping, structural studies, AMS, and thermobarometry indicate that the Late Cretaceous Big Hole Canyon pluton was emplaced as a laccolith at the top of a pre-existing fault-bend-fold in the frontal portion of the Sevier fold-thrust belt.

Tn5401, a new class II transposable element from Bacillus thuringiensis.

PubMed Central

Baum, J A

1994-01-01

A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, in the same orientation, encoding proteins of 36 kDa (306 residues) and 116 kDa (1,005 residues) and 53-bp terminal inverted repeats. The deduced amino acid sequence for the 36-kDa protein shows 24% sequence identity with the TnpI recombinase of the B. thuringiensis transposon Tn4430, a member of the phage integrase family of site-specific recombinases. The deduced amino acid sequence for the 116-kDa protein shows 42% sequence identity with the transposase of Tn3 but only 28% identity with the TnpA transposase of Tn4430. Two small open reading frames of unknown function, designated orf1 (85 residues) and orf2 (74 residues), were also identified. Southern blot analysis indicated that Tn5401, in contrast to Tn4430, is not commonly found among different subspecies of B. thuringiensis and is not typically associated with known insecticidal crystal protein genes. Transposition was studied with B. thuringiensis by using plasmid pEG922, a temperature-sensitive shuttle vector containing Tn5401. Tn5401 transposed to both chromosomal and plasmid target sites but displayed an apparent preference for plasmid sites. Transposition was replicative and resulted in the generation of a 5-bp duplication at the target site. Transcriptional start sites within Tn5401 were mapped by primer extension analysis. Two promoters, designated PL and PR, direct the transcription of orf1-orf2 and tnpI-tnpA, respectively, and are negatively regulated by TnpI. Sequence comparison of the promoter regions of Tn5401 and Tn4430 suggests that the conserved sequence element ATGTCCRCTAAY mediates TnpI binding and cointegrate resolution. The same element is contained within the 53-bp terminal

First Record of Culex coronator In Shelby County, Tennessee.

PubMed

Trimm, Amy; Insch, Andrew; Carlson, Ture

2017-12-01

Culex coronator has been identified in Shelby County, TN, for the first time as an established species. This is an important discovery since the range of Cx. coronator is unknown in Tennessee and it has been found positive for West Nile virus. Larvae and an adult were discovered during routine surveillance. Larvae were collected at 9 different locations, while the 1 adult captured was at the 10th isolated location.

75 FR 21983 - Standard Instrument Approach Procedures, and Takeoff Minimums and Obstacle Departure Procedures...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-27

..., RNAV (GPS) RWY 20, Amdt 1A Paris, TN, Henry County, ILS OR LOC/NDB RWY 2, Amdt 1 Paris, TN, Henry County, NDB RWY 2, Amdt 3 Paris, TN, Henry County, RNAV (GPS) RWY 2, Orig Paris, TN, Henry County, RNAV...

International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21▿

PubMed Central

Novais, Ângela; Baquero, Fernando; Machado, Elisabete; Cantón, Rafael; Peixe, Luísa; Coque, Teresa M.

2010-01-01

TEM-24 remains one of the most widespread TEM-type extended-spectrum β-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-ΔTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly

Testing the Extensional Detachment Paradigm: A Borehole Observatory in the Sevier Desert Basin, Utah

NASA Astrophysics Data System (ADS)

Christie-Blick, N.; Wernicke, B. P.

2007-12-01

The Sevier Desert basin, Utah represents a world-class target for scientific drilling and for the development of an in situ borehole observatory of active faulting, with potential for establishing that normal-sense slip can occur along a brittle low-angle fault and, by determining the conditions under which that may take place, for resolving the mechanical paradox associated with such structures. The Sevier Desert detachment was defined in the mid- 1970s on the basis seismic reflection data and commercial wells as the contact between Paleozoic carbonate rocks and Cenozoic basin fill over a depth range of ~0-4 km. Today, the interpreted fault is thought by most workers to root into the crust to the west, to have large estimated offset (< 47 km), to have been active over most of its history near its present 11° dip, and to be associated with contemporary surface extension (a 30- km-long zone of prominent Holocene fault scarps immediately west of Clear Lake). Although no seismicity has been documented on the detachment, its scale is consistent with earthquake magnitudes as large as M 7.0. A published alternative interpretation of the Paleozoic-Cenozoic contact as an unconformity rather than a fault has not been generally accepted. Deformation at detachment faults is commonly spatially restricted, and may have been missed in well cuttings. Exhumation of the detachment would have made it possible to remove critical footwall evidence prior to later sedimentary onlap, particularly at updip locations. The incomplete coverage and uneven quality of seismic reflection data on which the detachment interpretation depends, and an unresolved debate about stratigraphic ties to a critical well, leave room for discussion about interpretive details, including the possibility that deformation was distributed across several closely spaced faults. An apparent mismatch between stratigraphically based ages and fission-track evidence for the timing of footwall exhumation cannot be

Syntectonic Fluid-Rock Interactions Involving Surficial Waters in the Sevier Thrust Belt, Tendoy Mountains, Southwest Montana

NASA Astrophysics Data System (ADS)

Johnson, A. C.; Anastasio, D. J.; Bebout, G. E.

2002-05-01

Calcite veins and Mississippian carbonates from the Sevier thrust front record syntectonic meteoric fluid infiltration and hydrocarbon migration. The Tendoy and Four Eyes Canyon thrust sheets were emplaced onto the western margin of the Late Cretaceous Western Interior Seaway \\{WIS\\}. Low salinity \\{Tice = -0.6° C to +3.6° C\\} and low temperature \\{110° C +/- 10\\} fluids interacted with hanging-wall carbonates at a depth of 5km. Most veins have single or multiple generations of varying apertures, composed predominately of large euhedral crystals with some finer grained layers and protolith inclusions. Orientation analysis of mutually cross-cutting, high-angle vein sets suggest development concurrent with Four Eyes Canyon thrusting but prior to Tendoy thrusting. These vein sets are generally cut by later synfolding bed-parallel shear veins. Reactivation of both the bed-parallel and bed-perpendicular vein sets \\{strike parallel and strike perpendicular\\} in the Four Eyes Canyon thrust sheet occurred subsequent to Sevier compression, creating wide, coarse crystalline veins that often transect Sevier structures. Oxygen and Carbon isotope analyses of veins allow for reconstruction of fluid-rock interactions during thrust sheet emplacement and later reactivation. All veins and variably deformed host-rocks were microsampled and analyzed for δ 18OV-SMOW and δ 13CV-PDB. Small Tendoy veins \\{1mm-1cm wide\\} have calcite δ 18O values of +8.9 to +28.8‰ and calculated fluid \\{as H2O\\} of -8.3 to +11.6‰ \\{100° C\\}, -7.3 to +12.6‰ \\{110° C\\}, and -6.3 to +13.6‰ \\{120° C\\}. Four Eyes Canyon veins \\{1cm-3m wide\\} have calcite δ 18O values of +5.9 to +17.0‰ and calculated fluid of -11.3 to -0.2‰ \\{100° C\\}, -10.3 to +0.8‰ \\{110° C\\}, and -9.3 to +1.8‰ \\{120° C\\}. While there is significant variation in δ 18O there is relatively little systematic variation seen in δ 13C. Protolith carbonate has δ 18O values of +22.2‰ +/- 3

Analytical evaluation of point of care cTnT and clinical performances in an unselected population as compared with central laboratory highly sensitive cTnT.

PubMed

Dupuy, Anne Marie; Sebbane, Mustapha; Roubille, François; Coste, Thibault; Bargnoux, Anne Sophie; Badiou, Stéphanie; Kuster, Nils; Cristol, Jean Paul

2015-03-01

To report the analytical performances of the Radiometer AQT90 FLEX® cTnT assay (Neuilly-Plaisance, France) and to evaluate the concordance with hs-cTnT results from central laboratory for the diagnosis of acute myocardial infarction (AMI) at baseline and during a short follow-up among unselected patients admitted in emergency room or cardiology department. Analytical performances of AQT90 FLEX® cTnT immunoassay included imprecision study with determination of a coefficient of variation at 10% and 20%, linearity, and limit of detection. The concordance study was based on samples obtained from 170 consecutive patients with chest pain suggestive of acute coronary syndrome (ACS) admitted in the emergency room or cardiology department. The kinetic study (within 62 additional samples 3h later) was based on absolute delta criterion and the combination of relative change of 30% with absolute change of 7ng/L. The cTnT assay from Radiometer was evaluated as clinically usable, although less sensitive than the Roche hs-cTnT assay as demonstrated by the concordance and the kinetic studies. In non-selected population, the cTnT AQT Flex© assay on AQT90© with kinetic change at 3h, provides similar clinical classification of patients, particularly for AMI group as compared to central laboratory hs-cTnT assay and could be suitable for clinical use. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

76 FR 64086 - Availability of FY 10 Grantee Performance Evaluation Reports for the Eight States of EPA Region 4...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-17

... Conservation Resources, FL; Louisville Metro Air Pollution Control District, KY; Forsyth County Environmental... Carolina Regional Air Quality Agency, NC; Chattanooga-Hamilton County Air Pollution Control Bureau, TN; Shelby County Health Department, TN; Knox County Department of Air Quality Management, TN; and...

77 FR 52022 - Availability of FY 11 Grantee Performance Evaluation Reports for the Eight States of EPA Region 4...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-28

... Conservation Resources, FL; Louisville Metro Air Pollution Control District, KY; Forsyth County Environmental... Carolina Regional Air Quality Agency, NC; Chattanooga-Hamilton County Air Pollution Control Bureau, TN; Shelby County Health Department, TN; Knox County Department of Air Quality Management, TN; and...

Tn4556, a 6.8-kilobase-pair transposable element of Streptomyces fradiae.

PubMed Central

Chung, S T

1987-01-01

A 6.8-kilobase-pair (kbp) transposable element (Tn4556) was found in a neomycin-producing strain of Streptomyces fradiae. This element was first observed in two 30.3-kbp plasmids (pUC1123 and pUC1124) which arose when a thiostrepton resistance gene (1 kbp) was ligated with the BclI-2 fragment (22.5 kbp) that contains the origin of replication of phage SF1. The Tn4556 segment was deleted when these plasmids were transduced into another S. fradiae host with phage SF1. These deletion plasmids (pUC1210 and pUC1211) had copy numbers of less than 1 per chromosome and were unstable. In contrast, pUC1123 and pUC1124, with copy numbers of 12 to 15 per chromosome, respectively, were relatively stable. When pUC1210 and pUC1211 were reintroduced into S. fradiae by protoplast transformation, the Tn4556 element transposed again to the plasmids at numerous new locations in either of two orientations. A copy of Tn4556 was found in the S. fradiae chromosome by hybridization studies. It appears that Tn4556 originated from the chromosome, transposed into unstable pUC1210 and pUC1211, and made stable plasmids. A temperature-sensitive hybrid plasmid carrying a viomycin resistance derivative of Tn4556 (pMT660::Tn4556::vph) was constructed. When Streptomyces lividans UC8390 containing the hybrid plasmid was grown at 39 degrees C, Tn4556::vph (Tn4560) transposed to random positions in the host chromosome. Images PMID:2820925

Mini-Tn7 Insertion in Bacteria With Multiple glmS-Linked attTn7 Sites: Example Burkholderia Mallei ATCC 23344

DTIC Science & Technology

2006-06-27

INTRODUCTION Burkholderia mallei is the etiological agent of glanders and a highly evolved obligate zoonotic mammalian pathogen that naturally affects horses...mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344 Kyoung-Hee Choi1, David DeShazer2... glanders is a rare disease, B. mallei has received renewed attention because of its listing as a category B agent by the Centers for Disease Control

Occurrence and properties of composite transposon Tn2672: evolution of multiple drug resistance transposons.

PubMed Central

Hänni, C; Meyer, J; Iida, S; Arber, W

1982-01-01

We found Tn2671 (the 23-kb long IS1-flanked r-determinant of NR1-Basel) inserted into the ampicillin resistance gene bla of the Tn3-related transposon Tn902. The resulting 28-kilobase-long composite transposon Tn2672 (= Tn902 bla::Tn2671) is stable, and it translocates as a unit into various loci including IS1 of the resistance transfer factor of R100-1. These results are discussed with respect to the evolution of R plasmids providing multiple drug resistance. Images PMID:6281241

Tn5099, a xylE promoter probe transposon for Streptomyces spp.

PubMed Central

Hahn, D R; Solenberg, P J; Baltz, R H

1991-01-01

Tn5099, a promoter probe transposon for Streptomyces spp., was constructed by inserting a promoterless xylE gene and a hygromycin resistance gene into IS493. Tn5099 transposed into different sites in the Streptomyces griseofuscus genome, and the xylE reporter gene was expressed in some of the transposition mutants. Strains containing Tn5099 insertions that gave regulated expression of the xylE gene were identified. Images PMID:1653213

76 FR 40720 - CenterPoint Energy Gas Transmission Company, LLC; Notice of Availability of the Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-11

..., Hempstead, and Sevier Counties, Arkansas; and the installation of a pig \\1\\ launcher at the beginning of the new pipeline (MP 0.0) and a pig receiver at its terminus (MP 16.7). \\1\\ A ``pig'' is a tool that is...

An insight into the thermodynamic characteristics of human thrombopoietin complexation with TN1 antibody

PubMed Central

Shibazaki, Chie; Adachi, Motoyasu; Honjo, Eijiro; Tamada, Taro; Maeda, Yoshitake; Tahara, Tomoyuki; Kato, Takashi; Miyazaki, Hiroshi; Blaber, Michael; Kuroki, Ryota

2016-01-01

Abstract Human thrombopoietin (hTPO) primarily stimulates megakaryocytopoiesis and platelet production and is neutralized by the mouse TN1 antibody. The thermodynamic characteristics of TN1 antibody–hTPO complexation were analyzed by isothermal titration calorimetry (ITC) using an antigen‐binding fragment (Fab) derived from the TN1 antibody (TN1‐Fab). To clarify the mechanism by which hTPO is recognized by TN1‐Fab the conformation of free TN1‐Fab was determined to a resolution of 2.0 Å using X‐ray crystallography and compared with the hTPO‐bound form of TN1‐Fab determined by a previous study. This structural comparison revealed that the conformation of TN1‐Fab does not substantially change after hTPO binding and a set of 15 water molecules is released from the antigen‐binding site (paratope) of TN1‐Fab upon hTPO complexation. Interestingly, the heat capacity change (ΔCp) measured by ITC (−1.52 ± 0.05 kJ mol−1 K−1) differed significantly from calculations based upon the X‐ray structure data of the hTPO‐bound and unbound forms of TN1‐Fab (−1.02 ∼ 0.25 kJ mol−1 K−1) suggesting that hTPO undergoes an induced‐fit conformational change combined with significant desolvation upon TN1‐Fab binding. The results shed light on the structural biology associated with neutralizing antibody recognition. PMID:27419667

78 FR 72004 - Amendment of Class E Airspace; Tazewell, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-02

... Tazewell, TN, as new Standard Instrument Approach Procedures have been developed at New Tazewell Municipal Airport. This enhances the safety and management of aircraft operations at the airport. DATES: Effective..., TN. Airspace reconfiguration is necessary due to the development of the RNAV (GPS) RWY 7 approach and...

76 FR 20606 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-13

... source(s) Location of referenced ground [caret] Communities affected elevation ** Elevation in meters (MSL) Effective Modified Sevier County, Utah, and Incorporated Areas Albinus Canyon Approximately 400... Creek Split Flow Approximately 400 feet None +5435 Town of Joseph. downstream of State Highway 118. At...

PD-1 suppresses development of humoral responses that protect against Tn-bearing tumors

PubMed Central

Haro, Marcela A.; Littrell, Chad A.; Yin, Zhaojun; Huang, Xuefei; Haas, Karen M.

2017-01-01

Tn is a carbohydrate antigen uniquely exposed on tumor mucins and thus, an ideal target for immunotherapy. However, it has been difficult to elicit protective antibody responses against Tn antigen and other tumor associated carbohydrate antigens. Our study demonstrates this can be attributed to PD-1 immuno-inhibition. Our data show a major role for PD-1 in suppressing mucin- and Tn-specific B-cell activation, expansion, and antibody production important for protection against Tn-bearing tumor cells. These Tn/mucin-specific B cells belong to the innate-like B-1b cell subset typically responsible for T cell–independent antibody responses. Interestingly, PD-1–mediated regulation is B cell–intrinsic and CD4+ cells play a key role in supporting Tn/mucin-specific B cell antibody production in the context of PD-1 deficiency. Mucin-reactive antibodies produced in the absence of PD-1 inhibition largely belong to the IgM subclass and elicit potent antitumor effects via a complement-dependent mechanism. The identification of this role for PD-1 in regulating B cell–dependent antitumor immunity to Tn antigen highlights an opportunity to develop new therapeutic strategies targeting tumor associated carbohydrate antigens. PMID:27856425

Characterization of Tn6238 with a New Allele of aac(6′)-Ib-cr

PubMed Central

Quiroga, María P.; Orman, Betina; Errecalde, Laura; Kaufman, Sara

2015-01-01

Here, we report that the genetic structure of Tn1331 remained conserved in Argentina from 1989 to 2013 (72 of 73 isolates), with the exception being the plasmid-borne Tn1331-like transposon Tn6238 containing a new aac(6′)-Ib-cr allele recovered from a colistin-resistant Klebsiella pneumoniae clinical isolate. A bioinformatic analysis of aac(6′)-Ib-like gene cassettes suggests that this new aac(6′)-Ib-cr allele emerged through mutation or homologous recombination in the Tn1331 genetic platform. Tn6238 is a novel platform for the dissemination of aminoglycoside and fluoroquinolone resistance determinants. PMID:25691640

Registration of TN09-008 soybean cyst nematode resistant cultivar

USDA-ARS?s Scientific Manuscript database

The conventional soybean line TN09-008 (Reg. No. CV- , PI ) was released by University of Tennessee Agricultural Research in 2017 as a cultivar, based on high seed yield potential in Tennessee and the southern region. Soybean cultivar TN09-008 is resistant to HG types 1.2.5.7, 5.7, a...

Curriculum Outline for Tennessee Transition Model.

ERIC Educational Resources Information Center

Esch, B. J.

This curriculum outline for the Sevier County, Tennessee, transition program for special needs students provides goals and objectives for the following domains: domestic, vocational, community functioning, and recreation/leisure. The domestic domain covers personal hygiene/grooming, first aid, home nursing, birth control/pregnancy, parenting, drug…

Myofilament Calcium Sensitivity: Mechanistic Insight into TnI Ser-23/24 and Ser-150 Phosphorylation Integration

PubMed Central

Salhi, Hussam E.; Hassel, Nathan C.; Siddiqui, Jalal K.; Brundage, Elizabeth A.; Ziolo, Mark T.; Janssen, Paul M. L.; Davis, Jonathan P.; Biesiadecki, Brandon J.

2016-01-01

Troponin I (TnI) is a major regulator of cardiac muscle contraction and relaxation. During physiological and pathological stress, TnI is differentially phosphorylated at multiple residues through different signaling pathways to match cardiac function to demand. The combination of these TnI phosphorylations can exhibit an expected or unexpected functional integration, whereby the function of two phosphorylations are different than that predicted from the combined function of each individual phosphorylation alone. We have shown that TnI Ser-23/24 and Ser-150 phosphorylation exhibit functional integration and are simultaneously increased in response to cardiac stress. In the current study, we investigated the functional integration of TnI Ser-23/24 and Ser-150 to alter cardiac contraction. We hypothesized that Ser-23/24 and Ser-150 phosphorylation each utilize distinct molecular mechanisms to alter the TnI binding affinity within the thin filament. Mathematical modeling predicts that Ser-23/24 and Ser-150 phosphorylation affect different TnI affinities within the thin filament to distinctly alter the Ca2+-binding properties of troponin. Protein binding experiments validate this assertion by demonstrating pseudo-phosphorylated Ser-150 decreases the affinity of isolated TnI for actin, whereas Ser-23/24 pseudo-phosphorylation is not different from unphosphorylated. Thus, our data supports that TnI Ser-23/24 affects TnI-TnC binding, while Ser-150 phosphorylation alters TnI-actin binding. By measuring force development in troponin-exchanged skinned myocytes, we demonstrate that the Ca2+ sensitivity of force is directly related to the amount of phosphate present on TnI. Furthermore, we demonstrate that Ser-150 pseudo-phosphorylation blunts Ser-23/24-mediated decreased Ca2+-sensitive force development whether on the same or different TnI molecule. Therefore, TnI phosphorylations can integrate across troponins along the myofilament. These data demonstrate that TnI Ser-23

Geology of an Ordovician stratiform base-metal deposit in the Long Canyon Area, Blaine County, Idaho

USGS Publications Warehouse

Otto, B.R.; Zieg, G.A.

2003-01-01

In the Long Canyon area, Blaine County, Idaho, a strati-form base-metal-bearing gossan is exposed within a complexly folded and faulted sequence of Ordovician strata. The gossan horizon in graptolitic mudrock suggests preservation of bedded sulfides that were deposited by an Ordovician subaqueous hydrothermal system. Abrupt thickness changes and geochemi-cal zoning in the metal-bearing strata suggest that the gossan is near the source of the hydrothermal system. Ordovician sedimentary rocks at Long Canyon represent a coarsening-upward section that was deposited below wave base in a submarine depositional environment. The lowest exposed rocks represent deposition in a starved, euxinic basin and over-lying strata represent a prograding clastic wedge of terrigenous and calcareous detritus. The metalliferous strata are between these two types of strata. Strata at Long Canyon have been deformed by two periods of thrust faulting, at least three periods of normal faulting, and two periods of folding. Tertiary extensional faulting formed five subhorizontal structural plates. These low-angle fault-bounded plates truncate Sevier-age and possibly Antler-age thrust faults. The presence of gossan-bearing strata in the four upper plates suggests that there was only minor, although locally complex, stratigraphic displacement and rotation. The lack of correlative strata in the lowest plate suggests the displacement was greater than 2000 ft. The metalliferous strata were exposed to surface weathering, oxidation, and erosion prior to and during deposition of the Eocene Challis Volcanic Group. The orientations of erosional canyons formed during this early period of exposure were related to the orientations of Sevier-age thrust faults, and stream-channel gravel was deposited in the canyons. During this and subsequent intervals of exposure, sulfidic strata were oxi-dized to a minimum depth of 700 ft.

40 CFR 81.343 - Tennessee.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Johnson County X That portion of Knox County within a section of downtown Knoxville X Rest of Knox County.... Knoxville, TN: Anderson County This action is effective 3/8/2011 Attainment Blount County This action is.... Knoxville, TN: Anderson County Nonattainment. Blount County Nonattainment. Knox County Nonattainment. Loudon...

Magnitude, Timing, and Geometry of Extension in the Southern Sevier Desert Basin From Piercing Points, Seismic-Stratigraphic Reconstruction, and Deep well Data

NASA Astrophysics Data System (ADS)

Coogan, J. C.; Decelles, P. G.

2007-12-01

Palinspastic reconstruction of Mesozoic thrust sheets provides the main constraint for an estimated 47 km of Cenozoic extensional displacement along the Sevier Desert detachment (SDD) in the central Sevier Desert Basin. Hanging wall and footwall piercing points indicate that the SDD accommodated a minimum of 35 km of extensional displacement in the narrower southern part of the basin. The piercing points for the SDD are defined by the intersection of the SDD, the Canyon Range thrust (CRT), and a regional early Cenozoic erosion surface (ES). The hanging wall piercing point lies immediately northeast of the Cricket Mountains, where the SDD-CRT- ES intersection is narrowly defined by intersecting structure maps derived from published seismic reflection data. The footwall piercing point lies in the southern foothills of the Canyon Range, where the SDD breakaway plane is well constrained by an industry seismic line that lies within 2 km of the exposed intersection of the CRT with the base of the Oligocene Oak City Formation. Timing of extension in the southern Sevier Desert basin is constrained by a kinematic reconstruction of detachment and imbricate fault displacement, footwall uplift, and supradetachment sedimentation for Oligocene, Miocene, and Plio-Pleistocene seismic sequences. The reconstruction is centered on a seismic reflection and gravity interpretation along the published Pan Canadian profiles 2 and 3 that is tied to dated intervals in six industry wells. Fault restoration indicates that Oligocene and Miocene phases of slip each accounted for about 40 percent of the total displacement. Simultaneous backstripping of the Oligocene, Miocene, and Plio-Pleistocene supradetachment sequences records hanging wall subsidence simultaneous with footwall uplift, with a footwall burial history that is consistent with published Miocene apatite and zircon fission-track ages of footwall samples. The geometric evolution of the southern SDD extensional system is consistent

Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903.

PubMed Central

Grindley, N D; Joyce, C M

1980-01-01

The kanamycin resistance transposon Tn903 consists of a unique region of about 1000 base pairs bounded by a pair of 1050-base-pair inverted repeat sequences. Each repeat contains two Pvu II endonuclease cleavage sites separated by 520 base pairs. We have constructed derivatives of Tn903 in which this 520-base-pair fragment is deleted from one or both repeats. Those derivatives that lack both 520-base-pair fragments cannot transpose, whereas those that lack just one remain transposition proficient. One such transposable derivative, Tn903 delta I, has been selected for further study. We have determined the sequence of the intact inverted repeat. The 18 base pairs at each end are identical and inverted relative to one another, a structure characteristic of insertion sequences. Additional experiments indicate that a single inverted repeat from Tn903 can, in fact, transpose; we propose that this element be called IS903. To correlate the DNA sequence with genetic activities, we have created mutations by inserting a 10-base-pair DNA fragment at several sites within the intact repeat of Tn903 delta 1, and we have examined the effect of such insertions on transposability. The results suggest that IS903 encodes a 307-amino-acid polypeptide (a "transposase") that is absolutely required for transposition of IS903 or Tn903. Images PMID:6261245

40 CFR 81.343 - Tennessee.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Johnson County X That portion of Knox County within a section of downtown Knoxville X Rest of Knox County...: Hawkins County (2) Attainment. Sullivan County (2) Attainment. Knoxville, TN: Anderson County This action... secondary) Designated area Designation Date 1 Type Classification Date 1 Type Knoxville, TN: 2 Nonattainment...

76 FR 30962 - Notice of Availability of Draft Environmental Impact Statement for the Sigurd to Red Butte No. 2...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-27

...; UTU-83067] Notice of Availability of Draft Environmental Impact Statement for the Sigurd to Red Butte..., as amended, the Bureau of Land Management (BLM) has prepared a Draft Environmental Impact Statement... Service; State of Utah; Millard, Sevier, Beaver, Iron, and Washington counties, Utah; and the cities of St...

The diagnostic value of two commercially available human cTnI assays in goat kids with myocarditis.

PubMed

Karapinar, Tolga; Eroksuz, Yesari; Hayirli, Armagan; Beytut, Enver; Kaynar, Ozgur; Baydar, Ersoy; Sozdutmaz, Ibrahim; Isidan, Hakan

2016-03-01

Cardiac troponin I (cTnI) is a peripheral blood marker for myocardial damage. Because of the unavailability of goat-specific cTnI assays human cTnI assays may be validated for detection of myocarditis in goat kids. The purpose of the study was to evaluate 2 commercially available human cTnI assays in goat kids with myocardial damage, and to determine the cTnI expression in cardiac muscle. Plasma cTnI concentrations were measured in healthy goat kids (n = 7) and goat kids with myocardial damage (n = 8) using the Beckman Coulter Access Accu TnI and the Biomérieux Vidas Ultra. The results were correlated with gross necropsy and histopathologic findings, and cTnI immunhistochemistry in cardiac tissue. Macro- and microscopic findings confirmed myocardial damage in the myocarditis group. Mean plasma cTnI concentration was significantly higher in the myocarditis group than in the healthy control group (104.82 vs 0.02 ng/mL). The overall mean plasma cTnI concentration measured by Biomérieux Vidas Ultra (61.75 ng/mL, 95% CI: 19.55-103.95) was comparable to the mean measured by Beckman Coulter Access Accu TnI (50.08 ng/mL, 95% CI: 24.11-76.06), and cTnI concentrations measured by these assays were highly correlated (r = .977) with a -6.2% bias. Both assays were precise and accurate. The human-specific Beckman Coulter Access Accu TnI and the Biomérieux Vidas Ultra can be used for diagnostic confirmation of myocardial damage in caprine medicine. © 2016 American Society for Veterinary Clinical Pathology.

40 CFR 81.343 - Tennessee.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Johnson County X That portion of Knox County within a section of downtown Knoxville X Rest of Knox County... County (2) Attainment. Knoxville, TN: Anderson County This action is effective 3/8/2011 Attainment Blount... Date 1 Type Knoxville, TN: 2 Nonattainment Marginal. Anderson County (part) 2000 Census tracts: 202...

The Relationship of Diameter at Breast Height and Crown Diameter for Four Species Groups in Hardin County, Tennessee

Treesearch

Lawrence R. Gering; Dennis M. May

1995-01-01

A set of simple linear regression models for predicting diameter at breast height (dbh) from crown diamter and a set of similar models for predicting crown diamter from dbh were developed for four species groups in Harding County, TN. Data were obtained from 557 trees measured during hte 1989 USDA Southern Forest Experiment Station survey of the forest of Tennessee,...

The Levels and Distribution of TN, TP and TOC in the South China Sea

NASA Astrophysics Data System (ADS)

Wang, H.; Han, D.

2012-04-01

The marine biogeochemistries of carbon, nitrogen and phosphorous have come under increased scrutiny because of their close involvement in climate change and coastal eutrophication. The South China Sea is unique in that located in a subtropical zone, and therefore represents an important regime for biogeochemical studies. However, to our knowledge, few data are available for total nitrogen (TN), total phosphorous (TP) and total organic carbon (TOC) in South Sea, China. The present study aims to contribute to the knowledge of their status through investigating the level and distribution of TN, TP and TOC in South China Sea. A total of 108 seawater samples of 11 sites in south sea, china were collected during August 29- September 4, 2006. An automated and simultaneous method for determination of TN and TOC was applied to all seawater samples. The combined system allowed simultaneous determination for TOC and TN in the same sample using a single injection and provided low detection limits and excellent linear ranges for both TOC and TN. The risk of contamination has been remarkably reduced due to the minimal sample manipulation and automated analyses. And quantitative analyses of TP in seawater were accomplished by a typical chemical method. Concentration ranges of TN and TP were 0.06-0.67, and 0.003-0.071 mg/L, respectively, as well as that of TOC were 0.23-2.51mg/L. The values of TN and TP showed that the status of nutrition is relatively better in south china sea than other marine areas. Moreover, the upright change trend of TN concentration level as well as TP and TOC according to the experimental results at the total 11 sites are simultaneity studied. The concentration of TN initial increases with the increasing of the depth, later the value becomes almost constant. In contrast, the concentration of TOC reduces with the increasing of the depth, later the value becomes almost unchangeable. Compared with the trend of TN and TOC, that of TP appears relatively stable

78 FR 68060 - Availability of FY 12 Grantee Performance Evaluation Reports for the Eight States of EPA Region 4...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-13

... County Air Pollution Control Bureau, TN; Shelby County Health Department, TN; Knox County Department of... evaluations of eight state air pollution control programs (Alabama Department of Environmental Management... Department, FL; Pinellas County Parks and Conservation Resources, FL; Louisville Metro Air Pollution Control...

Transposon mutagenesis of Xylella fastidiosa by electroporation of Tn5 synaptic complexes.

PubMed

Guilhabert, M R; Hoffman, L M; Mills, D A; Kirkpatrick, B C

2001-06-01

Pierce's disease, a lethal disease of grapevine, is caused by Xylella fastidiosa, a gram-negative, xylem-limited bacterium that is transmitted from plant to plant by xylem-feeding insects. Strains of X. fastidiosa also have been associated with diseases that cause tremendous losses in many other economically important plants, including citrus. Although the complete genome sequence of X. fastidiosa has recently been determined, the inability to transform or produce transposon mutants of X. fastidiosa has been a major impediment to understanding pathogen-, plant-, and insect-vector interactions. We evaluated the ability of four different suicide vectors carrying either Tn5 or Tn10 transposons as well as a preformed Tn5 transposase-transposon synaptic complex (transposome) to transpose X. fastidiosa. The four suicide vectors failed to produce any detectable transposition events. Electroporation of transposomes, however, yielded 6 x 10(3) and 4 x 10(3) Tn5 mutants per microg of DNA in two different grapevine strains of X. fastidiosa. Molecular analysis showed that the transposition insertions were single, independent, stable events. Sequence analysis of the Tn5 insertion sites indicated that the transpositions occur randomly in the X. fastidiosa genome. Transposome-mediated mutagenesis should facilitate the identification of X. fastidiosa genes that mediate plant pathogenicity and insect transmission.

RadNet Air Data From Nashville, TN

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Nashville, TN from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

RadNet Air Data From Knoxville, TN

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Knoxville, TN from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

RadNet Air Data From Memphis, TN

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Memphis, TN from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

78 FR 27029 - Modification of Class C Airspace; Nashville International Airport; TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-09

...-0031; Airspace Docket No. 12-AWA-7] Modification of Class C Airspace; Nashville International Airport... modifies the Nashville International Airport, TN, Class C airspace area by removing a cutout from the... modify the Nashville International Airport, TN, Class C airspace area (78 FR 6257). Interested parties...

cTnT can be a useful marker for early detection of anthracycline cardiotoxicity.

PubMed

Kilickap, S; Barista, I; Akgul, E; Aytemir, K; Aksoyek, S; Aksoy, S; Celik, I; Kes, S; Tekuzman, G

2005-05-01

The level of serum cardiac troponin-T (cTnT) increases with myocardial damage. We sought to assess whether cTnT level could be a useful marker for the early detection of anthracycline cardiotoxicity. Forty-one patients who had been scheduled to receive anthracycline-containing combination chemotherapy were included in the study. Serum cTnT levels were measured before (baseline) and after the first cycle of chemotherapy, and again, after the last cycle of chemotherapy. In all patients, the left ventricular ejection fraction (LVEF), fractional shortening (FS), early peak flow/atrial flow velocity (E/A) ratio, and the isovolemic relaxation time (IRT) were measured echocardiographically, both before and after the completion of chemotherapy. LVEF and FS did not change in any patients. In 21 patients (49%), the E/A ratio decreased after therapy as compared to the pre-treatment values. The decrease in E/A ratio was more prominent in patients who were older than the mean age of our study group, which was 44 years. The post-treatment IRT was prolonged compared with the pretreatment IRT (94.0 +/- 2.0 versus 85.6 +/- 10.5 ms, respectively). cTnT levels after completion of therapy were elevated in 14 (34%) patients, and exceeded the upper limit of the normal range (>0.1 ng/ml) in only one patient. cTnT levels measured after completion of therapy were significantly higher, compared with those measured at baseline and after the first cycle of therapy. In the younger age group (< or =44 years old), there was a two-fold decrease in the E/A ratio in those patients whose cTnT levels increased during the therapy, when compared with those whose cTnT levels did not change (21% versus 43%, respectively). Increased serum cTnT level can be detected in the early stages of anthracycline therapy and it is associated with diastolic dysfunction of the left ventricle. Therefore, serum cTnT level could be a useful measure for early detection of anthracycline-induced cardiotoxicity.

Iceland's Grímsvötn volcano erupts

NASA Astrophysics Data System (ADS)

Showstack, Randy

2011-05-01

About 13 months after Iceland's Eyjafjallajökull volcano began erupting on 14 April 2010, which led to extensive air traffic closures over Europe, Grímsvötn volcano in southeastern took its turn. Iceland's most active volcano, which last erupted in 2004 and lies largely beneath the Vatnajökull ice cap, began its eruption activity on 21 May, with the ash plume initially reaching about 20 kilometers in altitude, according to the Icelandic Meteorological Office. Volcanic ash from Grímsvötn has cancelled hundreds of airplane flights and prompted U.S. president Barack Obama to cut short his visit to Ireland. As Eos went to press, activity at the volcano was beginning to subside.

Iceland: Grímsvötn Volcano

Atmospheric Science Data Center

2013-04-17

article title:  Grímsvötn Volcano Injects Ash into the Stratosphere     ... p.m. local time (1730 UTC) on Saturday, May 21, 2011. The volcano, located approximately 140 miles (220 kilometers) east of the capital ...

The novel conjugative transposon tn1207.3 carries the macrolide efflux gene mef(A) in Streptococcus pyogenes.

PubMed

Santagati, Maria; Iannelli, Francesco; Cascone, Carmela; Campanile, Floriana; Oggioni, Marco R; Stefani, Stefania; Pozzi, Gianni

2003-01-01

The macrolide efflux gene mef(A) of the Streptococcus pyogenes clinical strain 2812A was found to be carried by a 52-kb chromosomal genetic element that could be transferred by conjugation to the chromosome of other streptococcal species. The characteristics of this genetic element are typical of conjugative transposons and was named Tn1207.3. The size of Tn1207.3 was established by pulsed-field gel electrophoresis (PFGE), and DNA sequencing analysis showed that the 7,244 bp at the left end of Tn1207.3 were identical to those of the pneumococcal Tn1207.1 element. Tn1207.3-like genetic elements were found to be inserted at a single specific chromosomal site in 12 different clinical isolates S. pyogenes exhibiting the M phenotype of resistance to macrolides and carrying the mef(A) gene. Tn1207.3 was transferred from S. pyogenes 2812A to Streptococcus pneumoniae, and sequence analysis carried out on six independent transconjugants showed that insertion of Tn1207.3 in the pneumococcal genome always occurred at a single specific site as in Tn1207.1. Using MF2, a representative S. pneumoniae transconjugant, as a donor, Tn1207.3 was transferred again by conjugation to S. pyogenes and Streptococcus gordonii. The previously described nonconjugative element Tn1207.1 of S. pneumoniae appears to be a defective element, part of a longer conjugative transposon that carries mef(A) and is found in clinical isolates of S. pyogenes.

The Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD

PubMed Central

Choi, Ki Young; Spencer, Jeanelle M.; Craig, Nancy L.

2014-01-01

The excision of transposon Tn7 from a donor site and its insertion into its preferred target site, attachment site attTn7, is mediated by four Tn7-encoded transposition proteins: TnsA, TnsB, TnsC, and TnsD. Transposition requires the assembly of a nucleoprotein complex containing all four Tns proteins and the DNA substrates, the donor site containing Tn7, and the preferred target site attTn7. TnsA and TnsB together form the heteromeric Tn7 transposase, and TnsD is a target-selecting protein that binds specifically to attTn7. TnsC is the key regulator of transposition, interacting with both the TnsAB transposase and TnsD-attTn7. We show here that TnsC interacts directly with TnsB, and identify the specific region of TnsC involved in the TnsB–TnsC interaction during transposition. We also show that a TnsC mutant defective in interaction with TnsB is defective for Tn7 transposition both in vitro and in vivo. Tn7 displays cis-acting target immunity, which blocks Tn7 insertion into a target DNA that already contains Tn7. We provide evidence that the direct TnsB–TnsC interaction that we have identified also mediates cis-acting Tn7 target immunity. We also show that TnsC interacts directly with the target selector protein TnsD. PMID:24982178

Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 Purification and Tagmentation Protocol

PubMed Central

Hennig, Bianca P.; Velten, Lars; Racke, Ines; Tu, Chelsea Szu; Thoms, Matthias; Rybin, Vladimir; Besir, Hüseyin; Remans, Kim; Steinmetz, Lars M.

2017-01-01

Efficient preparation of high-quality sequencing libraries that well represent the biological sample is a key step for using next-generation sequencing in research. Tn5 enables fast, robust, and highly efficient processing of limited input material while scaling to the parallel processing of hundreds of samples. Here, we present a robust Tn5 transposase purification strategy based on an N-terminal His6-Sumo3 tag. We demonstrate that libraries prepared with our in-house Tn5 are of the same quality as those processed with a commercially available kit (Nextera XT), while they dramatically reduce the cost of large-scale experiments. We introduce improved purification strategies for two versions of the Tn5 enzyme. The first version carries the previously reported point mutations E54K and L372P, and stably produces libraries of constant fragment size distribution, even if the Tn5-to-input molecule ratio varies. The second Tn5 construct carries an additional point mutation (R27S) in the DNA-binding domain. This construct allows for adjustment of the fragment size distribution based on enzyme concentration during tagmentation, a feature that opens new opportunities for use of Tn5 in customized experimental designs. We demonstrate the versatility of our Tn5 enzymes in different experimental settings, including a novel single-cell polyadenylation site mapping protocol as well as ultralow input DNA sequencing. PMID:29118030

76 FR 18288 - Tennessee Disaster #TN-00048

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-01

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12497 and 12498] Tennessee Disaster TN-00048 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an... this disaster for physical damage is 12497 6 and for economic injury is 12498 0. The State which...

78 FR 48762 - Tennessee Disaster #TN-00076

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-09

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13696 and 13697] Tennessee Disaster TN-00076 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 08/02/2013. Incident: Severe Storms and...

75 FR 55833 - Tennessee Disaster #TN-00042

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-14

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12303 and 12304] Tennessee Disaster TN-00042 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 09/07/2010. Incident: Severe Storms and...

77 FR 51100 - Tennessee Disaster #TN-00068

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-23

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13215 and 13216] Tennessee Disaster TN-00068 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of TENNESSEE dated. 08/16/2012. Incident: Severe storms...

76 FR 20433 - Tennessee Disaster #TN-00048

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-12

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12497 and 12498] Tennessee Disaster TN-00048 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is a an amendment of the Administrative declaration of a disaster for the State of Tennessee dated 03/23/2011. Incident: Severe Storms and...

Two-stage soil infiltration treatment system for treating ammonium wastewaters of low COD/TN ratios.

PubMed

Lei, Zhongfang; Wu, Ting; Zhang, Yi; Liu, Xiang; Wan, Chunli; Lee, Duu-Jong; Tay, Joo-Hwa

2013-01-01

Soil infiltration treatment (SIT) is ineffective to treat ammonium wastewaters of total nitrogen (TN) > 100 mg l(-1). This study applied a novel two-stage SIT process for effective TN removal from wastewaters of TN>100 mg l(-1) and of chemical oxygen demand (COD)/TN ratio of 3.2-8.6. The wastewater was first fed into the soil column (stage 1) at hydraulic loading rate (HLR) of 0.06 m(3) m(-2) d(-1) for COD removal and total phosphorus (TP) immobilization. Then the effluent from stage 1 was fed individually into four soil columns (stage 2) at 0.02 m(3) m(-2) d(-1) of HLR with different proportions of raw wastewater as additional carbon source. Over the one-year field test, balanced nitrification and denitrification in the two-stage SIT revealed excellent TN removal (>90%) from the tested wastewaters. Copyright © 2012 Elsevier Ltd. All rights reserved.

An Enterobacter plasmid as a new genetic background for the transposon Tn1331

PubMed Central

Alavi, Mohammad R; Antonic, Vlado; Ravizee, Adrien; Weina, Peter J; Izadjoo, Mina; Stojadinovic, Alexander

2011-01-01

Background Genus Enterobacter includes important opportunistic nosocomial pathogens that could infect complex wounds. The presence of antibiotic resistance genes in these microorganisms represents a challenging clinical problem in the treatment of these wounds. In the authors’ screening of antibiotic-resistant bacteria from complex wounds, an Enterobacter species was isolated that harbors antibiotic-resistant plasmids conferring resistance to Escherichia coli. The aim of this study was to identify the resistance genes carried by one of these plasmids. Methods The plasmids from the Enterobacter isolate were propagated in E. coli and one of the plasmids, designated as pR23, was sequenced by the Sanger method using fluorescent dyeterminator chemistry on a genetic analyzer. The assembled sequence was annotated by search of the GenBank database. Results Plasmid pR23 is composed of the transposon Tn1331 and a backbone plasmid that is identical to the plasmid pPIGDM1 from Enterobacter agglomerans. The multidrug-resistance transposon Tn1331, which confers resistance to aminoglycoside and beta lactam antibiotics, has been previously isolated only from Klebsiella. The Enterobacter plasmid pPIGDM1, which carries a ColE1-like origin of replication and has no apparent selective marker, appears to provide a backbone for propagation of Tn1331 in Enterobacter. The recognition sequence of Tn1331 transposase for insertion into pPIGDM1 is the pentanucleotide TATTA, which occurs only once throughout the length of this plasmid. Conclusion Transposition of Tn1331 into the Enterobacter plasmid pPIGDM1 enables this transposon to propagate in this Enterobacter. Since Tn1331 was previously isolated only from Klebsiella, this report suggests horizontal transfer of this transposon between the two bacterial genera. PMID:22259249

Effects of HCM cTnI Mutation R145G on Troponin Structure and Modulation by PKA Phosphorylation Elucidated by Molecular Dynamics Simulations

PubMed Central

Lindert, Steffen; Cheng, Yuanhua; Kekenes-Huskey, Peter; Regnier, Michael; McCammon, J. Andrew

2015-01-01

Cardiac troponin (cTn) is a key molecule in the regulation of human cardiac muscle contraction. The N-terminal cardiac-specific peptide of the inhibitory subunit of troponin, cTnI (cTnI1-39), is a target for phosphorylation by protein kinase A (PKA) during β-adrenergic stimulation. We recently presented evidence indicating that this peptide interacts with the inhibitory peptide (cTnl137–147) when S23 and S24 are phosphorylated. The inhibitory peptide is also the target of the point mutation cTnI-R145G, which is associated with hypertrophic cardiomyopathy (HCM), a disease associated with sudden death in apparently healthy young adults. It has been shown that both phosphorylation and this mutation alter the cTnC-cTnI (C-I) interaction, which plays a crucial role in modulating contractile activation. However, little is known about the molecular-level events underlying this modulation. Here, we computationally investigated the effects of the cTnI-R145G mutation on the dynamics of cTn, cTnC Ca2+ handling, and the C-I interaction. Comparisons were made with the cTnI-R145G/S23D/S24D phosphomimic mutation, which has been used both experimentally and computationally to study the cTnI N-terminal specific effects of PKA phosphorylation. Additional comparisons between the phosphomimic mutations and the real phosphorylations were made. For this purpose, we ran triplicate 150 ns molecular dynamics simulations of cTnI-R145G Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, cTnI-R145G/S23D/S24D Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, and cTnI-R145G/PS23/PS24 Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, respectively. We found that the cTnI-R145G mutation did not impact the overall dynamics of cTn, but stabilized crucial Ca2+-coordinating interactions. However, the phosphomimic mutations increased overall cTn fluctuations and destabilized Ca2+ coordination. Interestingly, cTnI-R145G blunted the intrasubunit interactions between the cTnI N-terminal extension and the cTnI inhibitory

Effects of HCM cTnI mutation R145G on troponin structure and modulation by PKA phosphorylation elucidated by molecular dynamics simulations.

PubMed

Lindert, Steffen; Cheng, Yuanhua; Kekenes-Huskey, Peter; Regnier, Michael; McCammon, J Andrew

2015-01-20

Cardiac troponin (cTn) is a key molecule in the regulation of human cardiac muscle contraction. The N-terminal cardiac-specific peptide of the inhibitory subunit of troponin, cTnI (cTnI(1-39)), is a target for phosphorylation by protein kinase A (PKA) during β-adrenergic stimulation. We recently presented evidence indicating that this peptide interacts with the inhibitory peptide (cTnl(137-147)) when S23 and S24 are phosphorylated. The inhibitory peptide is also the target of the point mutation cTnI-R145G, which is associated with hypertrophic cardiomyopathy (HCM), a disease associated with sudden death in apparently healthy young adults. It has been shown that both phosphorylation and this mutation alter the cTnC-cTnI (C-I) interaction, which plays a crucial role in modulating contractile activation. However, little is known about the molecular-level events underlying this modulation. Here, we computationally investigated the effects of the cTnI-R145G mutation on the dynamics of cTn, cTnC Ca(2+) handling, and the C-I interaction. Comparisons were made with the cTnI-R145G/S23D/S24D phosphomimic mutation, which has been used both experimentally and computationally to study the cTnI N-terminal specific effects of PKA phosphorylation. Additional comparisons between the phosphomimic mutations and the real phosphorylations were made. For this purpose, we ran triplicate 150 ns molecular dynamics simulations of cTnI-R145G Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), cTnI-R145G/S23D/S24D Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), and cTnI-R145G/PS23/PS24 Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), respectively. We found that the cTnI-R145G mutation did not impact the overall dynamics of cTn, but stabilized crucial Ca(2+)-coordinating interactions. However, the phosphomimic mutations increased overall cTn fluctuations and destabilized Ca(2+) coordination. Interestingly, cTnI-R145G blunted the intrasubunit interactions between the cTnI N

76 FR 36166 - Tennessee Disaster Number TN-00051

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Role of Myofibril-Inducing RNA in cardiac TnT expression in developing Mexican axolotl

PubMed Central

Sferrazza, Gian-Franco; Zhang, Chi; Jia, Pingping; Lemanski, Sharon L.; Athauda, Gagani; Stassi, Alyssa; Halager, Kristine; Maier, Jennifer A.; Rueda-de-Leon, Elena; Gupta, Amit; Dube, Syamalima; Huang, Xupei; Prentice, Howard M.; Dube, Dipak K.; Lemanski, Larry F.

2007-01-01

The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene “c”, for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts.. Myofibril-Inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac Troponin-T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart PMID:17408593

Asphalt deposits and oil conditions in southwestern Arkansas: Chapter J in Contributions to economic geology, 1918, Part II, Mineral fuels

USGS Publications Warehouse

Miser, Hugh D.; Purdue, Albert Homer

1919-01-01

Seven asphalt deposits, three of which are in Pike County and four in Sevier County, in southwestern Arkansas, were examined by the writers during the progress of the field work for the De Queen-Caddo Gap folio, which is now in preparation. These deposits are all found at or near the same horizon in the Trinity formation. Only one has so far been developed into a mine from which asphalt has been shipped in commercial quantity. This deposit, which has been described by Hayes, 1 is about 2£ miles south-southeast of Pike, in Pike County. Of the other six deposits only three have been prospected.

Spatial variability assessment of soil nutrients in an intense agricultural area, a case study of Rugao County in Yangtze River Delta Region, China

NASA Astrophysics Data System (ADS)

Zhao, Yongcun; Xu, Xianghua; Darilek, Jeremy Landon; Huang, Biao; Sun, Weixia; Shi, Xuezheng

2009-05-01

Topsoil samples (0-20 cm) ( n = 237) were collected from Rugao County, China. Geostatistical variogram analysis, sequential Gaussian simulation (SGS), and principal component (PC) analysis were applied to assess spatial variability of soil nutrients, identify the possible areas of nutrient deficiency, and explore spatial scale of variability of soil nutrients in the county. High variability of soil nutrient such as soil organic matter (SOM), total nitrogen (TN), available P, K, Fe, Mn, Cu, Zn, and B concentrations were observed. Soil nutrient properties displayed significant differences in their spatial structures, with available Cu having strong spatial dependence, SOM and available P having weak spatial dependence, and other nutrient properties having moderate spatial dependence. The soil nutrient deficiency, defined here as measured nutrient concentrations which do not meet the advisory threshold values specific to the county for dominant crops, namely rice, wheat, and rape seeds, was observed in available K and Zn, and the deficient areas covered 38 and 11%, respectively. The first three PCs of the nine soil nutrient properties explained 62.40% of the total variance. TN and SOM with higher loadings on PC1 are closely related to soil texture derived from different parent materials. The PC2 combined intermediate response variables such as available Zn and P that are likely to be controlled by land use and soil pH. Available B has the highest loading on PC3 and its variability of concentrations may be primarily ascribed to localized anthropogenic influence. The amelioration of soil physical properties (i.e. soil texture) and soil pH may improve the availability of soil nutrients and the sustainability of the agricultural system of Rugao County.

Response of high-risk of recurrence/progression bladder tumours expressing sialyl-Tn and sialyl-6-T to BCG immunotherapy

PubMed Central

Lima, L; Severino, P F; Silva, M; Miranda, A; Tavares, A; Pereira, S; Fernandes, E; Cruz, R; Amaro, T; Reis, C A; Dall'Olio, F; Amado, F; Videira, P A; Santos, L; Ferreira, J A

2013-01-01

Background: High risk of recurrence/progression bladder tumours is treated with Bacillus Calmette-Guérin (BCG) immunotherapy after complete resection of the tumour. Approximately 75% of these tumours express the uncommon carbohydrate antigen sialyl-Tn (Tn), a surrogate biomarker of tumour aggressiveness. Such changes in the glycosylation of cell-surface proteins influence tumour microenvironment and immune responses that may modulate treatment outcome and the course of disease. The aim of this work is to determine the efficiency of BCG immunotherapy against tumours expressing sTn and sTn-related antigen sialyl-6-T (s6T). Methods: In a retrospective design, 94 tumours from patients treated with BCG were screened for sTn and s6T expression. In vitro studies were conducted to determine the interaction of BCG with high-grade bladder cancer cell line overexpressing sTn. Results: From the 94 cases evaluated, 36 had recurrence after BCG treatment (38.3%). Treatment outcome was influenced by age over 65 years (HR=2.668; (1.344–5.254); P=0.005), maintenance schedule (HR=0.480; (0.246–0.936); P=0.031) and multifocallity (HR=2.065; (1.033–4.126); P=0.040). sTn or s6T expression was associated with BCG response (P=0.024; P<0.0001) and with increased recurrence-free survival (P=0.001). Multivariate analyses showed that sTn and/or s6T were independent predictive markers of recurrence after BCG immunotherapy (HR=0.296; (0.148–0.594); P=0.001). In vitro studies demonstrated higher adhesion and internalisation of the bacillus to cells expressing sTn, promoting cell death. Conclusion: s6T is described for the first time in bladder tumours. Our data strongly suggest that BCG immunotherapy is efficient against sTn- and s6T-positive tumours. Furthermore, sTn and s6T expression are independent predictive markers of BCG treatment response and may be useful in the identification of patients who could benefit more from this immunotherapy. PMID:24064971

Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells.

PubMed

Muroi, K; Suda, T; Nakamura, M; Okada, S; Nojiri, H; Amemiya, Y; Miura, Y; Hakomori, S

1994-01-01

The epitopes Tn and sialosyl-Tn are expressed on erythrocytes of individuals with a very rare blood group, who often suffer from "Tn syndrome." We surveyed expression of Tn and sialosyl-Tn in normal blood cells, malignant transformed cells, and progenitor stem cells from bone marrow (BM). An anti-Tn antibody, IE3, and an anti-sialosyl-Tn antibody, TKH2, were used in this study. TKH2 reacted with erythroblasts, B cells, and a subset of CD4+ cells; but not with erythrocytes. Erythroblastic cell lines (K562, HEL, and UT7/EPO) and B-cell lines (Daudi, Raji, and B-cell lines transformed by Epstein-Barr virus) showed reactivity to TKH2. Similar results from the reactivity of TKH2 with transformed cells from leukemia patients and lymphoma patients were obtained; TKH2 reacted with blasts from erythroleukemia (M6; for 4 of 4 cases) and with lymphocytes from B-cell chronic lymphocytic leukemia (3 of 3), B-cell lymphoma (5 of 5), and CD4+ adult T-cell leukemia (4 of 4), but did not react with blasts from acute myeloid leukemia (M0 to M5; 0 of 22) or acute lymphoid leukemia (B-lymphoid leukemia, 0 of 11; T-lymphoid leukemia, 0 of 2; undifferentiated leukemia, 0 of 1). IE3 did not react with all of the tested cells. CD2-CD19-TKH2+ normal BM cells (BMC) contained blasts and various maturation stages of erythroblasts. The TKH2+ cells produced a large number of colony-forming unit-erythroid (CFU-E) colonies, whereas they produced a small number of burst-forming unit-erythroid colonies and CFU-granulocyte-macrophage colonies. CD34+ normal BMC did not express Tn and sialosyl-Tn. These findings suggest that sialosyl-Tn expresses in CFU-E to erythroblasts.

Julian Davies and the discovery of kanamycin resistance transposon Tn5.

PubMed

Berg, Douglas E

2017-04-01

This paper recounts some of my fond memories of a collaboration between Julian Davies and myself that started in 1974 in Geneva and that led to our serendipitous discovery of the bacterial kanamycin resistance transposon Tn5, and aspects of the lasting positive impact of our interaction and discovery on me and the community. Tn5 was one of the first antibiotic resistance transposons to be found. Its analysis over the ensuing decades provided valuable insights into mechanisms and control of transposition, and led to its use as a much-valued tool in diverse areas of molecular genetics, as also will be discussed here.

Structural Analysis of the Hg(II)-Regulatory Protein Tn501 MerR from Pseudomonas aeruginosa

NASA Astrophysics Data System (ADS)

Wang, Dan; Huang, Shanqing; Liu, Pingying; Liu, Xichun; He, Yafeng; Chen, Weizhong; Hu, Qingyuan; Wei, Tianbiao; Gan, Jianhua; Ma, Jing; Chen, Hao

2016-09-01

The metalloprotein MerR is a mercury(II)-dependent transcriptional repressor-activator that responds to mercury(II) with extraordinary sensitivity and selectivity. It’s widely distributed in both Gram-negative and Gram-positive bacteria but with barely detectable sequence identities between the two sources. To provide structural basis for the considerable biochemical and biophysical experiments previously performed on Tn501 and Tn21 MerR from Gram-negative bacteria, we analyzed the crystal structure of mercury(II)-bound Tn501 MerR. The structure in the metal-binding domain provides Tn501 MerR with a high affinity for mercury(II) and the ability to distinguish mercury(II) from other metals with its unique planar trigonal coordination geometry, which is adopted by both Gram-negative and Gram-positive bacteria. The mercury(II) coordination state in the C-terminal metal-binding domain is transmitted through the allosteric network across the dimer interface to the N-terminal DNA-binding domain. Together with the previous mutagenesis analyses, the present data indicate that the residues in the allosteric pathway have a central role in maintaining the functions of Tn501 MerR. In addition, the complex structure exhibits significant differences in tertiary and quaternary structural arrangements compared to those of Bacillus MerR from Gram-positive bacteria, which probably enable them to function with specific promoter DNA with different spacers between -35 and -10 elements.

76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

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A critical evaluation of the Beckman Coulter Access hsTnI: Analytical performance, reference interval and concordance.

PubMed

Pretorius, Carel J; Tate, Jillian R; Wilgen, Urs; Cullen, Louise; Ungerer, Jacobus P J

2018-05-01

We investigated the analytical performance, outlier rate, carryover and reference interval of the Beckman Coulter Access hsTnI in detail and compared it with historical and other commercial assays. We compared the imprecision, detection capability, analytical sensitivity, outlier rate and carryover against two previous Access AccuTnI assay versions. We established the reference interval with stored samples from a previous study and compared the concordances and variances with the Access AccuTnI+3 as well as with two commercial assays. The Access hsTnI had excellent analytical sensitivity with the calibration slope 5.6 times steeper than the Access AccuTnI+3. The detection capability was markedly improved with the SD of the blank 0.18-0.20 ng/L, LoB 0.29-0.33 ng/L and LoD 0.58-0.69 ng/L. All the reference interval samples had a result above the LoB value. At a mean concentration of 2.83 ng/L the SD was 0.28 ng/L (CV 9.8%). Carryover (0.005%) and outlier (0.046%) rates were similar to the Access AccuTnI+3. The combined male and female 99th percentile reference interval was 18.2 ng/L (90% CI 13.2-21.1 ng/L). Concordance amongst the assays was poor with only 16.7%, 19.6% and 15.2% of samples identified by all 4 assays as above the 99th, 97.5th and 95th percentiles. Analytical imprecision was a minor contributor to the observed variances between assays. The Beckman Coulter Access hsTnI assay has excellent analytical sensitivity and precision characteristics close to zero. This allows cTnI measurement in all healthy individuals and the capability to identify numerically small differences between serial samples as statistically significant. Concordance in healthy individuals remains poor amongst assays. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

High-sensitive cardiac troponin hs-TnT levels in sudden deaths related to atherosclerotic coronary artery disease.

PubMed

Beausire, Tim; Faouzi, Mohamed; Palmiere, Cristian; Fracasso, Tony; Michaud, Katarzyna

2018-06-04

Ischemic heart disease (IHD) related to atherosclerotic coronary artery disease (CAD) is one of the most prevalent causes of death in Europe. Postmortem evaluation of IHD remains a challenge because of possible non-specific autopsy finding in some autopsy cases, especially in early myocardial ischemia. High-sensitive cardiac troponin T (hs-TnT) is used today in clinical practice as the "gold standard" to diagnose the myocardial ischemia, and might also be applied as an ancillary tool for post-mortem evaluation. The goal of this study is to evaluate the diagnostic value of post-mortem serum hs-TnT assay in cases of sudden death related to IHD. We will also investigate the influence of cardiopulmonary resuscitation (CPR) attempts on post-mortem hs-TnT levels. The hs-TnT values in serum were retrospectively analysed in 85 autopsy data. 52 cases with clinical history and morphological results suggesting cardiac ischemia were included in the study group (mean age 53.5; age range 34-75) and 33 cases in the control group (mean age 40.4; age range 15-69). The group's statistical comparison was performed using logistic regression model. Our study showed a significant non-linear association between hs-TnT serum values and post-mortem diagnosis of sudden deaths related to IHD (p-value 0.005). The shape of the relationship is showing that the probability of death due to IHD increases quickly with a light level of hs-TnT (maximum around 90ng/L) then decreases slightly while remaining at high in values. No significant difference in the hs-TnT serum values was found between the CPR and the non-CPR cases (p-value 0.304). The measurement of hs-TnT serum values might be considered as an ancillary tool for the evaluation of death related to IHD, while taking necessary precautions in the interpretation of the results. Copyright © 2018 Elsevier B.V. All rights reserved.

Experimental manipulation of TN:TP ratiossuppress cyanobacterial biovolume and microcystinconcentration in large-scale in situ mesocosms

USGS Publications Warehouse

Harris, Theodore D.; Wilhelm, Frank M.; Graham, Jennifer L.; Loftin, Keith A.

2014-01-01

A global dataset was compiled to examine relations between the total nitrogen to total phosphorus ratio (TN:TP) and microcystin concentration in lakes and reservoirs. Microcystin concentration decreased as TN:TP ratios increased, suggesting that manipulation of the TN:TP ratio may reduce microcystin concentrations. This relationship was experimentally tested by adding ammonium nitrate to increase the TN:TP ratio in large-scale (70 m3), in situ mesocosms located in a eutrophic reservoir that routinely experiences toxic blooms of cyanobacteria. At a TN:TP ratio >75:1, chlorophytes dominated the phytoplankton community in the mesocosms, while cyanobacterial biovolume was significantly reduced and microcystin was not detected. In contrast, the unmanipulated reservoir was dominated by cyanobacteria, and microcystin was detected. Secchi depths were 1.1 to 1.8 times greater in the mesocosms relative to the reservoir. Cladoceran zooplankton had a larger body size (0.14 mm on average) in the mesocosms compared to conspecifics in the reservoir, which was likely related to the higher quality food. Combined, these empirical and experimental data indicate that although nutrient addition is counterintuitive to current cyanobacteria management practices, increasing the TN:TP ratio by adding nitrogen may be a potential short-term management strategy to reduce cyanobacteria and cyanotoxins when other alternatives (e.g., phosphorus reduction) are not possible. Additional experimental studies with careful controls are needed to define best management practices and identify any potential unintended consequences before nitrogen addition is implemented as a lake and reservoir management practice.

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76 FR 60802 - Foreign-Trade Zone 77-Memphis, TN; Withdrawal of Request for Temporary/Interim Manufacturing...

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Structural Analysis of the Hg(II)-Regulatory Protein Tn501 MerR from Pseudomonas aeruginosa

PubMed Central

Wang, Dan; Huang, Shanqing; Liu, Pingying; Liu, Xichun; He, Yafeng; Chen, Weizhong; Hu, Qingyuan; Wei, Tianbiao; Gan, Jianhua; Ma, Jing; Chen, Hao

2016-01-01

The metalloprotein MerR is a mercury(II)-dependent transcriptional repressor-activator that responds to mercury(II) with extraordinary sensitivity and selectivity. It’s widely distributed in both Gram-negative and Gram-positive bacteria but with barely detectable sequence identities between the two sources. To provide structural basis for the considerable biochemical and biophysical experiments previously performed on Tn501 and Tn21 MerR from Gram-negative bacteria, we analyzed the crystal structure of mercury(II)-bound Tn501 MerR. The structure in the metal-binding domain provides Tn501 MerR with a high affinity for mercury(II) and the ability to distinguish mercury(II) from other metals with its unique planar trigonal coordination geometry, which is adopted by both Gram-negative and Gram-positive bacteria. The mercury(II) coordination state in the C-terminal metal-binding domain is transmitted through the allosteric network across the dimer interface to the N-terminal DNA-binding domain. Together with the previous mutagenesis analyses, the present data indicate that the residues in the allosteric pathway have a central role in maintaining the functions of Tn501 MerR. In addition, the complex structure exhibits significant differences in tertiary and quaternary structural arrangements compared to those of Bacillus MerR from Gram-positive bacteria, which probably enable them to function with specific promoter DNA with different spacers between −35 and −10 elements. PMID:27641146

The crazy hollow formation (Eocene) of central Utah

USGS Publications Warehouse

Weiss, M.P.; Warner, K.N.

2001-01-01

The Late Eocene Crazy Hollow Formation is a fluviatile and lacustrine unit that was deposited locally in the southwest arm of Lake Uinta during and after the last stages of the lake the deposited the Green River Formation. Most exposures of the Crazy Hollow are located in Sanpete and Sevier Counties. The unit is characterized by a large variety of rock types, rapid facies changes within fairly short distances, and different lithofacies in the several areas where outcrops of the remnants of the formation are concentrated. Mudstone is dominant, volumetrically, but siltstone, shale, sandstone, conglomerate and several varieties of limestone are also present. The fine-grained rocks are mostly highly colored, especially in shades of yellow, orange and red. Sand grains, pebbles and small cobbles of well-rounded black chert are widespread, and "salt-and-pepper sandstone" is the conspicuous characteristic of the Crazy Hollow. The salt-and-pepper sandstone consists of grains of black chert, white chert, quartz and minor feldspar. The limestone beds and lenses are paludal and lacustrine in origin; some are fossiliferous, and contain the same fauna found in the Green River Formation. With trivial exceptions, the Crazy Hollow Formation lies on the upper, limestone member of the Green River Formation, and the beds of the two units are always accordant in attitude. The nature of the contact differs locally: at some sites there is gradation from the Green River to the Crazy Hollow; at others, rocks typical of the two units intertongue; elsewhere there is a disconformity between the two. A variety of bedrock units overlie the Crazy Hollow at different sites. In the southeasternmost districts it is overlain by the late Eocene formation of Aurora; in western Sevier County it is overlain by the Miocene-Pliocene Sevier River Formation; in northernmost Sanpete County it is overlain by the Oligocene volcanics of the Moroni Formation. At many sites bordering Sanpete and Sevier Valleys

Transponson Tn916 Mutagenesis in Bacillus anthracis,

DTIC Science & Technology

1987-11-10

Tngla, is described. Tng1a was transferred from Streptococcus 1aJaji strain DS16C1 to f. a VNR-1 by conjugation in a standard filter mating procedure...transposon, Tn916, mutagenesis, Bacillus, anthracis, subtilis. , Streptococcus , faecalis, aro 2.AUSrN ACT (Cautious no reverse efho if nece.at7r sd ideratfy...transferred from Streptococcus : faecalis strain DS16CI to B. anthracis VNR-1 by conjugation in a standard filter mating procedure. Tetracycline

The fully synthetic MAG-Tn3 therapeutic vaccine containing the tetanus toxoid-derived TT830-844 universal epitope provides anti-tumor immunity.

PubMed

Laubreton, Daphné; Bay, Sylvie; Sedlik, Christine; Artaud, Cécile; Ganneau, Christelle; Dériaud, Edith; Viel, Sophie; Puaux, Anne-Laure; Amigorena, Sebastian; Gérard, Catherine; Lo-Man, Richard; Leclerc, Claude

2016-03-01

Malignant transformations are often associated with aberrant glycosylation processes that lead to the expression of new carbohydrate antigens at the surface of tumor cells. Of these carbohydrate antigens, the Tn antigen is particularly highly expressed in many carcinomas, especially in breast carcinoma. We designed MAG-Tn3, a fully synthetic vaccine based on three consecutive Tn moieties that are O-linked to a CD4+ T cell epitope, to induce anti-Tn antibody responses that could be helpful for therapeutic vaccination against cancer. To ensure broad coverage within the human population, the tetanus toxoid-derived peptide TT830-844 was selected as a T-helper epitope because it can bind to various HLA-DRB molecules. We showed that the MAG-Tn3 vaccine, which was formulated with the GSK proprietary immunostimulant AS15 and designed for human cancer therapy, is able to induce an anti-Tn antibody response in mice of various H-2 haplotypes, and this response correlates with the ability to induce a specific T cell response against the TT830-844 peptide. The universality of the TT830-844 peptide was extended to new H-2 and HLA-DRB molecules that were capable of binding this T cell epitope. Finally, the MAG-Tn3 vaccine was able to induce anti-Tn antibody responses in cynomolgus monkeys, which targeted Tn-expressing tumor cells and mediated tumor cell death both in vitro and in vivo. Thus, MAG-Tn3 is a highly promising anticancer vaccine that is currently under evaluation in a phase I clinical trial.

Impacts of climate change on TN load and its control in a River Basin with complex pollution sources.

PubMed

Yang, Xiaoying; Warren, Rachel; He, Yi; Ye, Jinyin; Li, Qiaoling; Wang, Guoqing

2018-02-15

It is increasingly recognized that climate change could affect the quality of water through complex natural and anthropogenic mechanisms. Previous studies on climate change and water quality have mostly focused on assessing its impact on pollutant loads from agricultural runoff. A sub-daily SWAT model was developed to simulate the discharge, transport, and transformation of nitrogen from all known anthropogenic sources including industries, municipal sewage treatment plants, concentrated and scattered feedlot operations, rural households, and crop production in the Upper Huai River Basin. This is a highly polluted basin with total nitrogen (TN) concentrations frequently exceeding Class V of the Chinese Surface Water Quality Standard (GB3838-2002). Climate change projections produced by 16 Global Circulation Models (GCMs) under the RCP 4.5 and RCP 8.5 scenarios in the mid (2040-2060) and late (2070-2090) century were used to drive the SWAT model to evaluate the impacts of climate change on both the TN loads and the effectiveness of three water pollution control measures (reducing fertilizer use, constructing vegetative filter strips, and improving septic tank performance) in the basin. SWAT simulation results have indicated that climate change is likely to cause an increase in both monthly average and extreme TN loads in February, May, and November. The projected impact of climate change on TN loads in August is more varied between GCMs. In addition, climate change is projected to have a negative impact on the effectiveness of septic tanks in reducing TN loads, while its impacts on the other two measures are more uncertain. Despite the uncertainty, reducing fertilizer use remains the most effective measure for reducing TN loads under different climate change scenarios. Meanwhile, improving septic tank performance is relatively more effective in reducing annual TN loads, while constructing vegetative filter strips is more effective in reducing annual maximum monthly

Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs).

PubMed

Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

2014-01-01

Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons--Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element "captured" with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not

DRAFT LANDSAT DATA MOSAIC: MONTGOMERY COUNTY, TEXAS; HARRIS COUNTY, TEXAS; FORT BEND COUNTY, TEXAS; BRAZORIA COUNTY, TEXAS; GALVESTON COUNTY, TEXAS

EPA Science Inventory

This is a draft Landsat Data Mosaic, which contains remote sensing information for Montgomery County, Texas Harris County, Texas Fort Bend County, Texas Brazoria County, Texas Galveston County, and Texas Imagery dates on the following dates: October 6, 1999 and September 29, 200...

77 FR 13073 - Designation for the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA Areas

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-05

... the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA Areas AGENCY: Grain Inspection..., IA areas, Lincoln, Midsouth, and Sioux City, respectively were the sole applicants for designation to.../2015 Midsouth Memphis, TN (901) 942-3216 4/1/2012 3/31/2015 Sioux City Sioux City, IA......... (712...

The effect and biological mechanism of COD/TN ratio on nitrogen removal in a novel upflow microaerobic sludge reactor treating manure-free piggery wastewater.

PubMed

Li, Jianzheng; Meng, Jia; Li, Jiuling; Wang, Cheng; Deng, Kaiwen; Sun, Kai; Buelna, Gerardo

2016-06-01

A novel upflow microaerobic sludge reactor (UMSR) was constructed to treat manure-free piggery wastewater with high NH4(+)-N concentration and low COD/TN ratio, and the effect and biological mechanism of COD/TN ratio on nitrogen removal were investigated at a constant hydraulic retention time of 8h and 35°C. The results showed that the UMSR could treat the wastewater with a better synchronous removal of COD, NH4(+)-N and TN. The microaerobic UMSR allowed nitrifiers, and heterotrophic and autotrophic denitrifiers to thrive in the flocs, revealing a multiple nitrogen removal mechanism in the reactor. Both the nitrifiers and denitrifiers would be restricted by an influent COD/TN ratio more than 0.82, resulting in a decrease of TN removal in the UMSR. To get a TN removal over 80% with a TN load removal above 0.86kg/(m(3)·d) in the UMSR, the influent COD/TN ratio should be less than 0.70. Copyright © 2016 Elsevier Ltd. All rights reserved.

Establishment of an Arbitrary PCR for Rapid Identification of Tn917 Insertion Sites in Staphylococcus epidermidis: Characterization of Biofilm-Negative and Nonmucoid Mutants

PubMed Central

Knobloch, Johannes K.-M.; Nedelmann, Max; Kiel, Kathrin; Bartscht, Katrin; Horstkotte, Matthias A.; Dobinsky, Sabine; Rohde, Holger; Mack, Dietrich

2003-01-01

Transposon mutagenesis with the Enterococcus faecalis transposon Tn917 is a genetic approach frequently used to identify genes related with specific phenotypes in gram-positive bacteria. We established an arbitrary PCR for the rapid and easy identification of Tn917 insertion sites in Staphylococcus epidermidis with six independent, well-characterized biofilm-negative Tn917 transposon mutants, which were clustered in the icaADBC gene locus or harbor Tn917 in the regulatory gene rsbU. For all six of these mutants, short chromosomal DNA fragments flanking both transposon ends could be amplified. All fragments were sufficient to correctly identify the Tn917 insertion sites in the published S. epidermidis genomes. By using this technique, the Tn917 insertion sites of three not-yet-characterized biofilm-negative or nonmucoid mutants were identified. In the biofilm-negative and nonmucoid mutant M12, Tn917 is inserted into a gene homologous to the regulatory gene purR of Bacillus subtilis and Staphylococcus aureus. The Tn917 insertions of the nonmucoid but biofilm-positive mutants M16 and M20 are located in genes homologous to components of the phosphoenolpyruvate-sugar phosphotransferase system (PTS) of B. subtilis, S. aureus, and Staphylococcus carnosus, indicating an influence of the PTS on the mucoid phenotype in S. epidermidis. PMID:14532029

Comparison of the gentamicin resistance transposon Tn5281 with regions encoding gentamicin resistance in Enterococcus faecalis isolates from diverse geographic locations.

PubMed Central

Hodel-Christian, S L; Murray, B E

1992-01-01

The genetic determinant encoding gentamicin resistance (Gmr) on the beta-lactamase encoding plasmid pBEM10 of Enterococcus faecalis HH22 is carried on a transposon, termed Tn5281, that is highly related to the staphylococcal Gmr transposons Tn4001 found in Australian isolates of Staphylococcus aureus and Tn4031 found in United States isolates of Staphylococcus epidermidis. We have now studied plasmid DNA from Gmr strains of E. faecalis isolated from diverse geographical locations (Houston, Pennsylvania, Thailand, and Chile) by using restriction endonuclease analysis and DNA-DNA hybridization to determine whether other Gmr E. faecalis carry Tn5281 or a similar type of element. We also compared these enterococci to several United States isolates of Staphylococcus aureus with nonmobile Gmr determinants. Three E. faecalis isolates (from Houston and Chile) carried Tn5281-like elements, whereas two isolates (from Houston and Pennsylvania) had restriction endonuclease and DNA-DNA hybridization patterns more similar to those of the Tn4001-IS257 hybrid found in the nonmobile Gmr determinants in United States isolates of S. aureus. A strain from Thailand had a third pattern unrelated to either Tn5281 or the nonmobile Gmr determinants present in United States isolates of S. aureus. Our results demonstrate that there is both similarity and diversity between the Gmr determinant of strains of E. faecalis isolated in diverse geographic locations. Images PMID:1332593

Optimization of high-rate TN removal in a novel constructed wetland integrated with microelectrolysis system treating high-strength digestate supernatant.

PubMed

Guo, Luchen; He, Keli; Wu, Shubiao; Sun, Hao; Wang, Yanfei; Huang, Xu; Dong, Renjie

2016-08-01

The potential of high-rate TN removal in three aerated horizontal subsurface-flow constructed wetlands to treat high-strength anaerobic digestate supernatant was evaluated. Different strategies of intermittent aeration and effluent recirculation were applied to compare their effect on nitrogen depuration performance. Additional glucose supply and iron-activated carbon based post-treatment systems were established and examined, respectively, to further remove nitrate that accumulated in the effluents from aerated wetlands. The results showed that intermittent aeration (1 h on:1 h off) significantly improved nitrification with ammonium removal efficiency of 90% (18.1 g/(m(2)·d)), but limited TN removal efficiency (53%). Even though effluent recirculation (a ratio of 1:1) increased TN removal from 53% to 71%, the effluent nitrate concentration was still high. Additional glucose was used as a post-treatment option and further increased the TN removal to 82%; however, this implementation caused additional organic pollution. Furthermore, the iron-activated carbon system stimulated with a microelectrolysis process achieved greater than 85% effluent nitrate removal and resulted in 86% TN removal. Considering the high TN removal rate, aerated constructed wetlands integrated with a microelectrolysis-driven system show great potential for treating high-strength digestate supernatant. Copyright © 2016 Elsevier Ltd. All rights reserved.

Analysis of the Shallow Groundwater Flow System at Fire Island National Seashore, Suffolk County, New York

USGS Publications Warehouse

Schubert, Christopher E.

2010-01-01

. A variable-density model of the shallow aquifer system and adjacent marine surface waters was developed to simulate groundwater flow patterns and rates. Nitrogen loads from the shallow aquifer system were calculated from representative total nitrogen (TN) concentrations and simulated groundwater discharges to back-barrier estuaries and the ocean. The model simulates groundwater directions, velocities, and discharge rates under 2005 mean annual conditions. Groundwater budgets were developed for recharge areas of similar land use that contribute freshwater to back-barrier estuaries, the ocean, and subsea-discharge zones. Total freshwater discharge from the shallow aquifer system is about 43,500 cubic meters per day (m3/d) (79.8 percent) to back-barrier estuaries and about 10,200 m3/d (18.7 percent) to the ocean; about 836 m3/d (1.5 percent) may exit the system as subsea underflow. The total contribution of fresh groundwater to shoreline discharge zones amounts to about 53,700 m3/d (98.5 percent). The median age of freshwater discharged to back-barrier estuaries and the ocean was 3.4 years, and the 95th-percentile age was 20 years. The TN concentrations and loads under 2005 mean annual conditions for areas that contribute fresh groundwater to back-barrier estuaries and the ocean were calculated for the principal land uses on Fire Island. The overall TN load from the shallow aquifer system to shoreline discharge zones is about 16,200 kilograms per year (kg/yr) (82.2 percent) to back-barrier estuaries and about 3,500 kg/yr (17.8 percent) to the ocean. The overall TN load to marine surface waters amounts to about 19,700 kg/yr-roughly 6 percent of the annual TN load from shallow groundwater entering the South Shore Estuary Reserve (SSER) from the Suffolk County mainland, which is about 345,000 kg/yr. In contrast to the TN load from shallow groundwater for the SSER watershed, which annually yields about 353 kilograms per square kilometer (kg/km2), the overall TN loa

Comparison of cardiac TnI outliers using a contemporary and a high-sensitivity assay on the Abbott Architect platform.

PubMed

Ryan, J B; Southby, S J; Stuart, L A; Mackay, R; Florkowski, C M; George, P M

2014-07-01

Assays for cardiac troponin (cTn) have undergone improvements in sensitivity and precision in recent years. Increased rates of outliers, however, have been reported on various cTn platforms, typically giving irreproducible, falsely higher results. We aimed to evaluate the outlier rate occurring in patients with elevated cTnI using a contemporary and high-sensitivity assay. All patients with elevated cTnI (up to 300 ng/L) performed over a 21-month period were assayed in duplicate. A contemporary assay (Abbott STAT Troponin-I) was used for the first part of the study and subsequently a high-sensitivity assay (Abbott STAT High-Sensitive Troponin-I) was used. Outliers exceeded a calculated critical difference (CD) (CD = z × √2 × SDAnalytical) where z = 3.5 (for probability of 0.0005) and critical outliers also were on a different side of the decision level. The respective outlier and critical outlier rates were 0.22% and 0.10% for the contemporary assay (n = 4009) and 0.18% and 0.13% for the high-sensitivity assay (n = 3878). There was no significant reduction in outlier rate between the two assays (χ(2) = 0.034, P = 0.854). Fifty-six percent of outliers occurred in samples where cTn was an 'add-on' test (and was stored and refrigerated prior to assay). Despite recent improvements in cTn methods, outliers (including critical outliers) still occur at a low rate in both a contemporary and high-sensitivity cTnI assay. Laboratory and clinical staff should be aware of this potential analytical error, particularly in samples with suboptimal sample handling such as add-on tests. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Grímsvötn Volcano Injects Ash into the Stratosphere

NASA Image and Video Library

2011-05-24

NASA Terra spacecraft captured this image of Grímsvötn, the most active of Iceland volcanoes, which began erupting around 5:30 p.m. local time 1730 UTC on Saturday, May 21, 2011, east of the capital city of Reykjavik.

Comparison of Friction Characteristics on TN and VA Mode Alignment Films with Friction Force Microscopy

NASA Astrophysics Data System (ADS)

Kwak, Musun; Chung, Hanrok; Kwon, Hyukmin; Kim, Jehyun; Han, Daekyung; Yi, Yoonseon; Lee, Sangmun; Lee, Chulgu; Cha, Sooyoul

Using frictional force microscopy (FFM), the friction surface characteristics were compared between twisted nematic (TN) mode and vertical alignment (VA) mode alignment films (AFs). The friction asymmetry was detected depending on temperature conditions on TN mode AF, but not on VA mode AF. The difference between two modes was explained by leaning intermolecular repulsion caused by the pre-tilt angle uniformity and the density of side chain. No level difference according to temperature conditions appeared when the pre-tilt angle were measured after liquid crystal (LC) injection.

Dynamic Equilibrium of Cardiac Troponin C's Hydrophobic Cleft and Its Modulation by Ca2+ Sensitizers and a Ca2+ Sensitivity Blunting Phosphomimic, cTnT(T204E).

PubMed

Schlecht, William; Dong, Wen-Ji

2017-10-18

Several studies have suggested that conformational dynamics are important in the regulation of thin filament activation in cardiac troponin C (cTnC); however, little direct evidence has been offered to support these claims. In this study, a dye homodimerization approach is developed and implemented that allows the determination of the dynamic equilibrium between open and closed conformations in cTnC's hydrophobic cleft. Modulation of this equilibrium by Ca 2+ , cardiac troponin I (cTnI), cardiac troponin T (cTnT), Ca 2+ -sensitizers, and a Ca 2+ -desensitizing phosphomimic of cTnT (cTnT(T204E) is characterized. Isolated cTnC contained a small open conformation population in the absence of Ca 2+ that increased significantly upon the addition of saturating levels of Ca 2+ . This suggests that the Ca 2+ -induced activation of thin filament arises from an increase in the probability of hydrophobic cleft opening. The inclusion of cTnI increased the population of open cTnC, and the inclusion of cTnT had the opposite effect. Samples containing Ca 2+ -desensitizing cTnT(T204E) showed a slight but insignificant decrease in open conformation probability compared to samples with cardiac troponin T, wild type [cTnT(wt)], while Ca 2+ sensitizer treated samples generally increased open conformation probability. These findings show that an equilibrium between the open and closed conformations of cTnC's hydrophobic cleft play a significant role in tuning the Ca 2+ sensitivity of the heart.

Correlating Calcium Binding, Förster Resonance Energy Transfer, and Conformational Change in the Biosensor TN-XXL

PubMed Central

Geiger, Anselm; Russo, Luigi; Gensch, Thomas; Thestrup, Thomas; Becker, Stefan; Hopfner, Karl-Peter; Griesinger, Christian; Witte, Gregor; Griesbeck, Oliver

2012-01-01

Genetically encoded calcium indicators have become instrumental in imaging signaling in complex tissues and neuronal circuits in vivo. Despite their importance, structure-function relationships of these sensors often remain largely uncharacterized due to their artificial and multimodular composition. Here, we describe a combination of protein engineering and kinetic, spectroscopic, and biophysical analysis of the Förster resonance energy transfer (FRET)-based calcium biosensor TN-XXL. Using fluorescence spectroscopy of engineered tyrosines, we show that two of the four calcium binding EF-hands dominate the FRET output of TN-XXL and that local conformational changes of these hands match the kinetics of FRET change. Using small-angle x-ray scattering and NMR spectroscopy, we show that TN-XXL changes from a flexible elongated to a rigid globular shape upon binding calcium, thus resulting in FRET signal output. Furthermore, we compare calcium titrations using fluorescence lifetime spectroscopy with the ratiometric approach and investigate potential non-FRET effects that may affect the fluorophores. Thus, our data characterize the biophysics of TN-XXL in detail and may form a basis for further rational engineering of FRET-based biosensors. PMID:22677394

78 FR 48078 - Proposed Amendment of Class E Airspace; Tazewell, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-07

...: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: This action proposes to amend Class E Airspace at Tazewell, TN, as new Standard Instrument Approach Procedures have been developed at New Tazewell Municipal Airport. This action would enhance the safety and...

78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-23

...-Cities Regional Airport, Tri-Cities, TN. Exclusionary language was omitted in the final rule published in... herein to include the corrective language. Since the regulatory text, as currently described, penetrates... FAA has determined that this regulation only involves an established body of technical regulations for...

Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi

2010-06-20

Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZ{alpha}-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cellsmore » as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.« less

Carcinoma autoantigens T and Tn and their cleavage products interact with Gal/GalNAc-specific receptors on rat Kupffer cells and hepatocytes.

PubMed

Schlepper-Schäfer, J; Springer, G F

1989-10-09

We studied interactions of isolated Thomsen-Friedenreich (T)- and Tn-specific glycoproteins with the Gal/GalNAc-specific receptors on rat Kupffer cells and compared them to those with rat hepatocytes. Immunoreactive T and Tn are specific pancarcinoma epitopes. Electron microscopy of gold-labelled T and Tn antigens revealed their specific binding to Kupffer cells, followed by their uptake via the coated pit/vesicle pathway of receptor-mediated endocytosis. Preincubation of Kupffer cells with GalNAc and GalNAc-BSA, but not GlcNAc or GlcNAc-BSA specifically inhibited binding of the T and Tn glycoproteins. Desialylated, isologous erythrocytes (T RBC) are known to bind to the Gal/GalNAc receptors of rat Kupffer cells and hepatocytes. This attachment was specifically inhibited by T and Tn in a concentration-dependent manner: 50% T RBC-Kupffer cell contacts were inhibited at 8.5.10(-6) mM T and 8.5.10(-5) mM Tn antigen concentrations, respectively. The corresponding figures for hepatocytes were 6.10(-6) mM T and 1.2.10(-6) mM Tn antigen. Amino-terminal cleavage products of the T glycoprotein, possessing clusters terminating in non-reducing Gal/GalNAc, inhibited T RBC binding to Kupffer cells and hepatocytes usually at 10(-2) to 10(-5) mM concentrations, whereas GalNAc, galactose and galactose glycosides inhibited at millimolar concentrations. Galactose-unrelated carbohydrates were inactive at concentrations greater than or equal to 50 mM.

77 FR 31372 - Proposed Flood Hazard Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-25

... West Baxter Avenue, Knoxville, TN 37917. Leon County, Texas, and Incorporated Areas Maps Available for.... Unincorporated Areas of Leon County.... Leon County Judge's Office, 130 East St. Marys, Centerville, TX 75833...

75 FR 65584 - Proposed Amendment of Class E Airspace; Savannah, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-26

...-1047; Airspace Docket No. 10-ASO-37] Proposed Amendment of Class E Airspace; Savannah, TN AGENCY...; Telephone: 1- 800-647-5527; Fax: 202-493-2251. You must identify the Docket Number FAA-2010-1047; Airspace... the proposal. Communications should identify both docket numbers (FAA Docket No. FAA-2010-1047...

IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration.

PubMed

Stewart, Barry J; Wardle, Simon J; Haniford, David B

2002-08-15

The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This strand transfer transpososome (STT) does appear to support an intermolecular disintegration reaction, but only at a very low level. Strikingly, assembly of the STT is not dependent on IHF, a host protein that is required for de novo assembly of all previously characterized Tn10 transpososomes. We suggest that disintegration substrates are able to form both transposon end and target type contacts with transposase because of their enhanced conformational flexibility. This probably allows the conformation of DNA within the complex that prevents the destructive disintegration reaction, and is responsible for relaxing the DNA sequence requirements for STT formation relative to other Tn10 transpososomes.

IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration

PubMed Central

Stewart, Barry J.; Wardle, Simon J.; Haniford, David B.

2002-01-01

The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This strand transfer transpososome (STT) does appear to support an intermolecular disintegration reaction, but only at a very low level. Strikingly, assembly of the STT is not dependent on IHF, a host protein that is required for de novo assembly of all previously characterized Tn10 transpososomes. We suggest that disintegration substrates are able to form both transposon end and target type contacts with transposase because of their enhanced conformational flexibility. This probably allows the conformation of DNA within the complex that prevents the destructive disintegration reaction, and is responsible for relaxing the DNA sequence requirements for STT formation relative to other Tn10 transpososomes. PMID:12169640

Incised valley fill interpretation for Mississippian Black Hand Sandstone, Appalachian Basin, USA: Implications for glacial eustasy at Kinderhookian-Osagean (Tn2-Tn3) boundary

USGS Publications Warehouse

Matchen, D.L.; Kammer, T.W.

2006-01-01

Lower Mississippian strata of east-central Ohio are predominantly fine-grained marine deposits of the Cuyahoga and Logan formations. Within these sediments is the Black Hand Sandstone of the Cuyahoga Formation. The Black Hand Sandstone is a multistory, crossbedded, coarse-grained conglomeratic sandstone. The contact between the Black Hand Sandstone and the subjacent Cuyahoga Formation is sharp and scoured, with intraclasts of the Cuyahoga Formation incorporated into the basal Black Hand Sandstone. The Black Hand Sandstone was previously thought to represent a distributary channel deposit; however, the combination of lithofacies and architectural elements indicates deposition in a braided stream setting. The Cuyahoga Formation was deposited in a shallow marine setting. The erosional basal contact of the Black Hand Sandstone and the juxtaposition of fluvial and marine sediments suggests a sequence boundary. The geographic distribution of the Black Hand Sandstone combined with the evidence for a sequence boundary suggests deposition in an incised valley. The age of the Black Hand Sandstone is key to inferring the causes of valley incision. The Black Hand Sandstone is nearly devoid of body fossils, necessitating a biostratigraphic analysis of the surrounding Cuyahoga and Logan formations. Analysis indicates the Logan Formation is early Osagean age. Data from the Cuyahoga Formation suggest a Kinderhookian age with a possible transition to the Osagean in the uppermost Cuyahoga Formation. This constrains the age of the Black Hand Sandstone to the transition at the Kinderhookian-Osagean boundary. Recent reports indicate late Kinderhookian (Tournaisian, Tn2) Gondwanan glaciation based upon tillites and sharp excursions in stable-isotope curves. A glacio-eustatic fall in sea level is inferred to have caused incision of the Cuyahoga Formation, followed by deposition of the Black Hand Sandstone and Logan Formation during the subsequent sea level rise. The associated

KPC-4 Is Encoded within a Truncated Tn4401 in an IncL/M Plasmid, pNE1280, Isolated from Enterobacter cloacae and Serratia marcescens

PubMed Central

Bryant, Kendall A.; Van Schooneveld, Trevor C.; Thapa, Ishwor; Bastola, Dhundy; Williams, Laurina O.; Safranek, Thomas J.; Hinrichs, Steven H.; Rupp, Mark E.

2013-01-01

We describe the transfer of blaKPC-4 from Enterobacter cloacae to Serratia marcescens in a single patient. DNA sequencing revealed that KPC-4 was encoded on an IncL/M plasmid, pNE1280, closely related to pCTX-M360. Further analysis found that KPC-4 was encoded within a novel Tn4401 element (Tn4401f) containing a truncated tnpA and lacking tnpR, ISKpn7 left, and Tn4401 IRL-1, which are conserved in other Tn4401 transposons. This study highlights the continued evolution of Tn4401 transposons and movement to multiple plasmid backbones that results in acquisition by multiple species of Gram-negative bacilli. PMID:23070154

Comparison of the new high sensitive cardiac troponin T with myoglobin, h-FABP and cTnT for early identification of myocardial necrosis in the acute coronary syndrome.

PubMed

Kurz, Kerstin; Giannitsis, Evangelos; Becker, Maike; Hess, Georg; Zdunek, Dietmar; Katus, Hugo A

2011-03-01

We sought to determine the performance of the new high sensitivity cardiac troponin T assay (TnThs) for early diagnosis of myocardial infarction in patients with suspected acute coronary syndrome (ACS) and compare it with the fourth generation cTnT assay, myoglobin and heart-type fatty acid binding protein (h-FABP). Ninety-four patients with diagnosis of suspected ACS without ST-segment elevation admitted to our chest pain unit were included. Patients were divided according to time from onset of symptoms to presentation into an early presenter group (<4 h) and a late presenter group (≥4 h). A median of six samples (range 2-8) were available per patient. The diagnostic performance of TnThs was assessed using ROC analysis. Areas under the curve (AUC) of baseline and follow-up results of TnThs, cTnT, myoglobin, and h-FABP were compared using c statistics. The TnThs assay allows an excellent prediction of non-ST-segment elevation myocardial infarction (non-STEMI) at presentation, particularly among late presenters. A follow-up sample improves diagnostic performance in a time-dependent manner. The AUC of TnThs was superior to cTnT at all time points. The performance of TnThs was at least as good as myoglobin and h-FABP at presentation and during follow-up. A baseline sample of TnThs allows an earlier prediction of non-STEMI than the less sensitive and precise fourth generation cTnT assay. Probably, this excellent performance of TnThs at baseline and follow-up could obviate the need for other early markers of necrosis in future.

Radioprotection of the Brain White Matter by Mn(III) N-Butoxyethylpyridylporphyrin-Based Superoxide Dismutase Mimic MnTnBuOE-2-PyP5+

PubMed Central

Weitzel, Douglas H.; Tovmasyan, Artak; Ashcraft, Kathleen A.; Rajic, Zrinka; Weitner, Tin; Liu, Chunlei; Li, Wei; Buckley, Anne F.; Prasad, Mark R.; Young, Kenneth H.; Rodriguiz, Ramona M.; Wetsel, William C.; Peters, Katherine B.; Spasojevic, Ivan; Herndon, James E.; Batinic-Haberle, Ines; Dewhirst, Mark W.

2015-01-01

Cranial irradiation is a standard therapy for primary and metastatic brain tumors. A major drawback of radiotherapy (RT), however, is long-term cognitive loss that affects quality of life. Radiation-induced oxidative stress in normal brain tissue is thought to contribute to cognitive decline. We evaluated the effectiveness of a novel mimic of superoxide dismutase enzyme (SOD), MnTnBuOE-2-PyP5+ (Mn(III) meso-tetrakis(N-n-butoxyethylpyridinium-2-yl)porphyrin), to provide long-term neuroprotection following 8 Gy of whole brain irradiation. Long-term RT damage can only be assessed by brain imaging and neurocognitive studies. C57BL/6J mice were treated with MnTnBuOE-2-PyP5+ before and after RT and evaluated three months later. At this time point, drug concentration in the brain was 25 nmol/L. Mice treated with MnTnBuOE-2-PyP5+/RT exhibited MRI evidence for myelin preservation in the corpus callosum compared with saline/RT treatment. Corpus callosum histology demonstrated a significant loss of axons in the saline/RT group that was rescued in the MnTnBuOE-2-PyP5+/RT group. In addition, the saline/RT groups exhibited deficits in motor proficiency as assessed by the rotorod test and running wheel tests. These deficits were ameliorated in groups treated with MnTnBuOE-2-PyP5+/RT. Our data demonstrate that MnTnBuOE-2-PyP5+ is neuroprotective for oxidative stress damage caused by radiation exposure. In addition, glioblastoma cells were not protected by MnTnBuOE-2-PyP5+ combination with radiation in vitro. Likewise, the combination of MnTnBuOE-2-PyP5+ with radiation inhibited tumor growth more than RT alone in flank tumors. In summary, MnTnBuOE-2-PyP5+ has dual activity as a neuroprotector and a tumor radiosensitizer. Thus, it is an attractive candidate for adjuvant therapy with RT in future studies with patients with brain cancer. PMID:25319393

The relationship between the expression of TN and the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis

PubMed Central

Jiang, Guohua; Zhang, Yinshun; Sun, Xianjie

2018-01-01

Abstract Background: This study was conducted with the aim to investigate the relationship between Tetranectin (TN) and efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis. Methods: Ninety-two patients with traumatic kyphosis admitted in our hospital from February 2014 to June 2016 were included whose serum TN levels were examined by ELISA. Using the mean level of TN as cut-off value, patients were classified into TN high level group (group I) and TN low level group (group II). The observation indexes, including operation time, intra-operational loss of blood, Cobb angle, postoperative complications and recurrence rate of kyphosis within post-operational 6 months were recorded for comparison. Results: TN level was significantly higher in group I [(6.19 ± 0.33) μmol/L] than that in group II [(5.29 ± 0.34) μmol/L] (P < .05). There was no significant difference in average age, sex, lesion site and average time from injury to operation between the two groups (all P > 0.05). Compared to group II, operation time in group I was significantly shortened (5.02 ± 1.15 VS 4.58 ± 0.53, P = .023), the intra-operational loss of blood decreased (2418.56 ± 362.06 VS 2235.84 ± 325.63, P = .013), post-operational Cobb angle decreased (11.10 ± 1.31 VS 6.93 ± 1.04, P = .000), and the incidence of postoperative complications (nail-breaking, rod-breaking and looseness) and recurrence rate decreased (18.8% VS 4.5%, P = .036; 10.4% VS 0.0%, P = .028). Conclusion: Serum TN level is proved to be related to the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis, and may serve as a possible indicator for clinical treatment. PMID:29384840

The relationship between the expression of TN and the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis.

PubMed

Jiang, Guohua; Zhang, Yinshun; Sun, Xianjie

2018-02-01

This study was conducted with the aim to investigate the relationship between Tetranectin (TN) and efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis. Ninety-two patients with traumatic kyphosis admitted in our hospital from February 2014 to June 2016 were included whose serum TN levels were examined by ELISA. Using the mean level of TN as cut-off value, patients were classified into TN high level group (group I) and TN low level group (group II). The observation indexes, including operation time, intra-operational loss of blood, Cobb angle, postoperative complications and recurrence rate of kyphosis within post-operational 6 months were recorded for comparison. TN level was significantly higher in group I [(6.19 ± 0.33) μmol/L] than that in group II [(5.29 ± 0.34) μmol/L] (P < .05). There was no significant difference in average age, sex, lesion site and average time from injury to operation between the two groups (all P > 0.05). Compared to group II, operation time in group I was significantly shortened (5.02 ± 1.15 VS 4.58 ± 0.53, P = .023), the intra-operational loss of blood decreased (2418.56 ± 362.06 VS 2235.84 ± 325.63, P = .013), post-operational Cobb angle decreased (11.10 ± 1.31 VS 6.93 ± 1.04, P = .000), and the incidence of postoperative complications (nail-breaking, rod-breaking and looseness) and recurrence rate decreased (18.8% VS 4.5%, P = .036; 10.4% VS 0.0%, P = .028). Serum TN level is proved to be related to the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis, and may serve as a possible indicator for clinical treatment.

Tn (N-acetyl-d-galactosamine-O-serine/threonine) immunization protects against hyperoxia-induced lung injury in adult mice through inhibition of the nuclear factor kappa B activity.

PubMed

Chen, Chung-Ming; Hwang, Jaulang; Chou, Hsiu-Chu; Shiah, Her-Shyong

2018-06-01

Prolonged hyperoxia exposure leads to inflammation and acute lung injury. Since hyperoxia activates nuclear factor kappa B (NF-κB) and proinflammatory mediators in lung fibroblasts and murine lungs, and proinflammatory cytokines upregulate Tn (N-acetyl-d-galactosamine-O-serine/threonine) expression in human gingival fibroblasts. We hypothesized connections exist between Tn expression and inflammation regulation. Thus, we immunized adult mice with Tn antigen to examine whether Tn vaccine can protect against hyperoxia-induced lung injury by inhibiting NF-κB activity and cytokine expression through the action of anti-Tn antibodies. Five-week-old female C57BL/6NCrlBltw mice were subcutaneously immunized with Tn antigen four times at biweekly intervals, and one additional immunization was performed at 1 week after the fourth immunization. Four days after the last immunization, mice were exposed to room air (RA) or hyperoxia (100% O 2 ) for up to 96 h. Four study groups were examined: carrier protein + RA (n = 6), Tn vaccine + RA (n = 6), carrier protein + O 2 (n = 6), and Tn vaccine + O 2 (n = 5). We observed that hyperoxia exposure reduced body weight, increased alveolar protein and cytokine (interleukin-6 and tumor necrosis factor-α) levels, increased mean linear intercept (MLI) values and lung injury scores, and increased lung NF-κB activity. By contrast, Tn immunization increased serum anti-Tn antibody titers and reduced the cytokine levels, MLI values, and lung injury scores. Furthermore, the alleviation of lung injury was accompanied by a reduction in NF-κB activity. Therefore, we proposed that Tn immunization attenuates hyperoxia-induced lung injury in adult mice by inhibiting the NF-κB activity. Copyright © 2018 Elsevier B.V. All rights reserved.

Age and evolution of thin-skinned deformation in Zacatecas, Mexico: Sevier orogeny evidence in the Mexican Fold-Thrust Belt

NASA Astrophysics Data System (ADS)

Ramírez-Peña, César Francisco; Chávez-Cabello, Gabriel

2017-07-01

Integrating U-Pb ages from zircons of syn- and postectonic intrusives emplaced in folded pre- and synorogenic marine sedimentary rocks, it is proposed that thin-skinned deformation in the Concepción del Oro salient of the Mexican Fold Thrust Belt in northern Zacatecas, Mexico, was active between 92 and 71.6 Ma. The intrusives Pico de Teyra and El Peñuelo (U-Pb zircon ages: 76.9 and 72.5 Ma) show internal tectonic foliations and horizontal shear zones that cut off aplitic veins, which apparently developed syntectonically to thin-skinned deformation. Other intrusives like Saltillito (71.6 Ma) and Concepción del Oro are clearly postectonic because they are undeformed internally, cut regional structures and are younger than syntectonic plutons. Biostratigraphic ages reported for synorogenic sediments (Concepción del Oro and Parras formations) indicate that regional thin-skinned deformation was active between Early Turonian and Late Campanian, which is in agreement with syn and postectonic intrusive emplacement ages in the area. Nevertheless, the thin-skinned structures are disrupted by a younger NNW-SSE high angle reverse and normal faults that uplifts the San Julián Block in the west and truncate the Concepción del Oro salient, suggesting a post-Paleocene thick-skinned stage of deformation. In this work, we propose that style and age of thin-skinned deformation is similar to the Sevier orogeny in the Rocky Mountains.

In-house preparation of lectin panel and detection of Tn polyagglutination.

PubMed

Das, Sudipta Sekhar

2015-01-01

Polyagglutination is a condition in which red cells are agglutinated by ABO-compatible adult human sera, but not by cord blood sera and may be acquired or inherited. Lectins are invaluable reagents in the investigation of red cells polyagglutination. We prepared in-house lectin panel and confirmed Tn polyagglutination in a pregnant lady. The lady was anemic and refused blood transfusion elsewhere due to serological discrepancy. We found ABO discrepancy and an incompatible minor cross-match in the initial investigation and suspected polyagglutination. Confirmation of polyagglutination was done using adult and cord sera. We then used the in-house lectin panels to detect the type of polyagglutination. The agglutination pattern with the various lectins was suggestive of Tn polyagglutination, which was further supported by the enzyme study. Most blood banks in India lack commercial lectin panels because of cost and procurement difficulty. Lectins play an important role in the diagnosis and differentiation of polyagglutination and immunohematological management of patient. The important and basic lectins can be prepared in-house using specific raw seeds following standardized protocol.

Novel Structure of Enterococcus faecium-Originated ermB-Positive Tn1546-Like Element in Staphylococcus aureus.

PubMed

Wan, Tsai-Wen; Hung, Wei-Chun; Tsai, Jui-Chang; Lin, Yu-Tzu; Lee, Hao; Hsueh, Po-Ren; Lee, Tai-Fen; Teng, Lee-Jene

2016-10-01

We determined the resistance determinants in 274 erythromycin-resistant methicillin-susceptible Staphylococcus aureus (MSSA) isolates during a 13-year period, 2000 to 2012. The resistance phenotypes, inducible macrolide-lincosamide-streptogramin (iMLS), constitutive MLS (cMLS), and macrolide-streptogramin (MS) resistance phenotypes, were examined by a double-disk diffusion D test. The ermB gene was more frequent (35%; 97/274) than ermC (27%; 75/274) or ermA (21%; 58/274). All 97 ermB-positive isolates harbored Tn551 and IS1216V The majority (89/97) of ermB-positive isolates displayed the cMLS phenotype and carried mobile element structure (MES)-like structures, which has been previously reported in sequence type 59 (ST59) methicillin-resistant S. aureus (MRSA). The remaining 8 ermB-carrying isolates, belonging to ST7 (n = 4), ST5 (n = 3), and ST59 (n = 1), were sasK intact and did not carry MES-like structures. Unlike a MES-like structure that was located on the chromosome, the ermB elements on sasK-intact isolates were located on plasmids by S1 nuclease pulsed-field gel electrophoresis (PFGE) analysis and conjugation tests. Sequence data for the ermB-containing region (14,566 bp) from ST59 NTUH_3874 revealed that the best match was a Tn1546-like element in plasmid pMCCL2 DNA (GenBank accession number AP009486) of Macrococcus caseolyticus Tn1546 is recognized as an enterococcal transposon and was known from the vancomycin resistance gene cluster in vancomycin-resistant Enterococcus (VRE). So far, acquisitions of Tn1546 in S. aureus have occurred in clonal complex 5 (CC5) MRSA, but not in MSSA. This is the first report that MSSA harbors an Enterococcus faecium-originated ermB-positive Tn1546-like element located on a plasmid. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes

PubMed Central

Bevacqua, R. J.; Fernandez-Martin, R.; Canel, N. G.; Gibbons, A.; Texeira, D.; Lange, F.; Vans Landschoot, G.; Savy, V.; Briski, O.; Hiriart, M. I.; Grueso, E.; Ivics, Z.; Taboga, O.; Kues, W. A.; Ferraris, S.

2017-01-01

Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes. In the case of Tn5, the transposome complex of transposon nucleic acid and Tn5 protein was injected. In the case of SB, the supercoiled plasmids encoding a transposon and the SB transposase were co-injected. In vitro produced bovine zygotes were used to establish the cytoplasmic injection conditions. The in vitro cultured blastocysts were evaluated for reporter gene expression and genotyped. Subsequently, both transposon systems were injected in seasonally available ovine zygotes, employing transposons carrying the recombinant human factor IX driven by the beta-lactoglobulin promoter. The Tn5 approach did not result in transgenic lambs. In contrast, the Sleeping Beauty injection resulted in 2 lambs (29%) carrying the transgene. Both animals exhibited cellular mosaicism of the transgene. The extraembryonic tissues (placenta or umbilical cord) of three additional animals were also transgenic. These results show that transpositional transgenesis by cytoplasmic injection of SB transposon components can be applied for the production of transgenic lambs of pharmaceutical interest. PMID:28301581

20. MEMBER 'A' SHOWS TENON AS USED IN POST 'A' ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. MEMBER 'A' SHOWS TENON AS USED IN POST 'A' (TN-159A-19), MEMBER 'B' IS BEAM 'B' IN TN-159A-19 AND SHOWS METHOD OF JOINING THESE MEMBERS. MEMBER 'C' SHOWS MORTISE IN BEAM 'B'. - Caleb Crosby Threshing Barn, Noeton (moved to Norris Dam State Park, Lake City), Morristown, Hamblen County, TN

Temperature-dependent instability of the cTnI subunit in NIST SRM2921 characterized by tryptic peptide mapping.

PubMed

van der Burgt, Yuri E M; Cobbaert, Christa M; Dalebout, Hans; Smit, Nico; Deelder, André M

2012-08-01

In this study temperature-dependent instability of the cTnI subunit of the three-protein complex NIST SRM2921 was demonstrated using a mass spectrometric tryptic peptide mapping approach. The results were compared to the cTnI subunit obtained as a protein standard from Calbiochem with identical amino acid sequence. Both the three-protein complex from NIST as well as the cTnI subunit were incubated at elevated temperatures and then evaluated with respect to the primary sequence. The corresponding peptide maps were analyzed using LC-MS/MS. From a Mascot database search in combination with "semiTrypsin" tolerance it was found that two peptide backbone cleavages had occurred in subunit cTnI in NIST SRM2921 material upon incubation at 37°C, namely between amino acids at 148/149 and 194/195. The Calbiochem standard did not show increased levels of "unexpected" peptides in tryptic peptide maps. One of the two peptide backbone cleavages could also be monitored using a "single-step" MALDI-MS approach, i.e. without the need for peptide separation. The amount of degradation appeared rather constant in replicate temperature-instability experiments. However, for accurate quantification internal labelled standards are needed. Copyright © 2012 Elsevier B.V. All rights reserved.

MnTnBuOE-2-PyP protects normal colorectal fibroblasts from radiation damage and simultaneously enhances radio/chemotherapeutic killing of colorectal cancer cells

PubMed Central

Kosmacek, Elizabeth A.; Chatterjee, Arpita; Tong, Qiang; Lin, Chi; Oberley, Rebecca E.

2016-01-01

Manganese porphyrins have been shown to be potent radioprotectors in a variety of cancer models. However, the mechanism as to how these porphyrins protect normal tissues from radiation damage still remains largely unknown. In the current study, we determine the effects of the manganese porphyrin, MnTnBuOE-2-PyP, on primary colorectal fibroblasts exposed to irradiation. We found that 2 Gy of radiation enhances the fibroblasts' ability to contract a collagen matrix, increases cell size and promotes cellular senesence. Treating fibroblasts with MnTnBuOE-2-PyP significantly inhibited radiation-induced collagen contraction, preserved cell morphology and also inhibited cellular senescence. We further showed that MnTnBuOE-2-PyP enhanced the overall viability of the fibroblasts following exposure to radiation but did not protect colorectal cancer cell viability. Specifically, MnTnBuOE-2-PyP in combination with irradiation, caused a significant decrease in tumor clonogenicity. Since locally advanced rectal cancers are treated with chemoradiation therapy followed by surgery and non-metastatic anal cancers are treated with chemoradiation therapy, we also investigated the effects of MnTnBuOE-2-PyP in combination with radiation, 5-fluorouracil with and without Mitomycin C. We found that MnTnBuOE-2-PyP in combination with Mitomycin C or 5-fluorouracil further enhances those compounds' ability to suppress tumor cell growth. When MnTnBuOE-2-PyP was combined with the two chemotherapeutics and radiation, we observed the greatest reduction in tumor cell growth. Therefore, these studies indicate that MnTnBuOE-2-PyP could be used as a potent radioprotector for normal tissue, while at the same time enhancing radiation and chemotherapy treatment for rectal and anal cancers. PMID:27119354

MnTnBuOE-2-PyP protects normal colorectal fibroblasts from radiation damage and simultaneously enhances radio/chemotherapeutic killing of colorectal cancer cells.

PubMed

Kosmacek, Elizabeth A; Chatterjee, Arpita; Tong, Qiang; Lin, Chi; Oberley-Deegan, Rebecca E

2016-06-07

Manganese porphyrins have been shown to be potent radioprotectors in a variety of cancer models. However, the mechanism as to how these porphyrins protect normal tissues from radiation damage still remains largely unknown. In the current study, we determine the effects of the manganese porphyrin, MnTnBuOE-2-PyP, on primary colorectal fibroblasts exposed to irradiation. We found that 2 Gy of radiation enhances the fibroblasts' ability to contract a collagen matrix, increases cell size and promotes cellular senesence. Treating fibroblasts with MnTnBuOE-2-PyP significantly inhibited radiation-induced collagen contraction, preserved cell morphology and also inhibited cellular senescence. We further showed that MnTnBuOE-2-PyP enhanced the overall viability of the fibroblasts following exposure to radiation but did not protect colorectal cancer cell viability. Specifically, MnTnBuOE-2-PyP in combination with irradiation, caused a significant decrease in tumor clonogenicity. Since locally advanced rectal cancers are treated with chemoradiation therapy followed by surgery and non-metastatic anal cancers are treated with chemoradiation therapy, we also investigated the effects of MnTnBuOE-2-PyP in combination with radiation, 5-fluorouracil with and without Mitomycin C. We found that MnTnBuOE-2-PyP in combination with Mitomycin C or 5-fluorouracil further enhances those compounds' ability to suppress tumor cell growth. When MnTnBuOE-2-PyP was combined with the two chemotherapeutics and radiation, we observed the greatest reduction in tumor cell growth. Therefore, these studies indicate that MnTnBuOE-2-PyP could be used as a potent radioprotector for normal tissue, while at the same time enhancing radiation and chemotherapy treatment for rectal and anal cancers.

Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus.

PubMed

Kambarev, Stanimir; Pecorari, Frédéric; Corvec, Stéphane

2018-02-09

Streptococcus gallolyticus ssp. gallolyticus (Sgg) is a commensal bacterium and an opportunistic pathogen. In humans it has been clinically associated with the incidence of colorectal cancer (CRC) and epidemiologically recognized as an emerging cause of infective endocarditis (IE). The standard therapy of Sgg includes the administration of a penicillin in combination with an aminoglycoside. Even though penicillin-resistant isolates have still not been reported, epidemiological studies have shown that this microbe is a reservoir of multiple acquired genes, conferring resistance to tetracyclines, aminoglycosides, macrolides and glycopeptides. However, the underlying antibiotic resistance mobilome of Sgg remains poorly understood. To investigate the mobile genetic basis of antibiotic resistance in multiresistant clinical Sgg. Isolate NTS31106099 was recovered from a patient with IE and CRC at Nantes University Hospital, France and studied by Illumina WGS and comparative genomics. Molecular epidemiology of the identified mobile element(s) was performed using antibiotic susceptibility testing (AST), PCR, PFGE and WGS. Mobility was investigated by PCR and filter mating. Two novel conjugative transposons, Tn6263 and Tn6331, confer aminoglycoside/macrolide co-resistance in clinical Sgg. They display classical family Tn916/Tn1545 modular architecture and harbour an aph(3')-III→sat4→ant(6)-Ia→erm(B) multiresistance gene cluster, related to pRE25 of Enterococcus faecium. These and/or closely related elements are highly prevalent among genetically heterogeneous clinical isolates of Sgg. Previously unknown Tn916-like mobile genetic elements conferring aminoglycoside/macrolide co-resistance make Sgg, collectively with other gut Firmicutes such as enterococci and eubacteria, a potential laterally active reservoir of these antibiotic resistance determinants among the mammalian gastrointestinal microbiota. © The Author(s) 2018. Published by Oxford University Press on behalf

Interruption of capsule production in Streptococcus pneumonia serotype 3 by insertion of transposon Tn916.

PubMed Central

Watson, D A; Musher, D M

1990-01-01

Transposon Tn916 mutagenesis was used to produce mutant strains of Streptococcus pneumoniae serotype 3 that lacked only a polysaccharide capsule. Southern blotting, DNA-DNA hybridization, and immunochemical analyses demonstrated that the presence of a single copy of Tn916 was sufficient to produce unencapsulation. The 50% lethal dose for such mutants was greater than 5 x 10(7) CFU, as opposed to a 50% lethal dose of 1 CFU for wild-type strains. These experiments outline an effective method for targeting genes in S. pneumoniae by transposon interruption and provide molecular evidence to support the longstanding hypothesis that the capsule is the principal virulence factor in this pathogen. Images PMID:2167295

Characterization of Tn1546 in vancomycin-resistant Enterococcus faecium isolated from canine urinary tract infections: evidence of gene exchange between human and animal enterococci.

PubMed

Simjee, S; White, D G; McDermott, P F; Wagner, D D; Zervos, M J; Donabedian, S M; English, L L; Hayes, J R; Walker, R D

2002-12-01

Thirty-five enterococcal isolates were recovered from dogs diagnosed with urinary tract infections at the Michigan State University Veterinary Teaching Hospital over a 2-year period (1996 to 1998). Isolated species included Enterococcus faecium (n = 13), Enterococcus faecalis (n = 7), Enterococcus gallinarum (n = 11), and Enterococcus casseliflavus (n = 4). Antimicrobial susceptibility testing revealed several different resistance phenotypes, with the majority of the enterococcal isolates exhibiting resistance to three or more antibiotics. One E. faecium isolate, CVM1869, displayed high-level resistance to vancomycin (MIC > 32 micro g/ml) and gentamicin (MIC > 2,048 micro g/ml). Molecular analysis of this isolate revealed the presence of Tn1546 (vanA), responsible for high-level vancomycin resistance, and Tn5281 carrying aac6'-aph2", conferring high-level aminoglycoside resistance. Pulsed-field gel electrophoresis analysis revealed that CVM1869 was a canine E. faecium clone that had acquired Tn1546, perhaps from a human vancomycin-resistant E. faecium. Transposons Tn5281 and Tn1546 were located on two different conjugative plasmids. Sequence analysis revealed that in Tn1546, ORF1 had an 889-bp deletion and an IS1216V insertion at the 5' end and an IS1251 insertion between vanS and vanH. To date, this particular form of Tn1546 has only been described in human clinical vancomycin-resistant enterococcus isolates unique to the United States. Additionally, this is the first report of a vancomycin-resistant E. faecium isolated from a companion animal in the United States.

77 FR 59994 - Nuclear Fuel Services, Inc., Erwin, TN; Issuance of License Renewal

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-01

... NUCLEAR REGULATORY COMMISSION [Docket No. 70-143; NRC-2009-0435] Nuclear Fuel Services, Inc., Erwin, TN; Issuance of License Renewal AGENCY: U.S. Nuclear Regulatory Commission. ACTION: Notice of... Nuclear Material Safety and Safeguards, U.S. Nuclear Regulatory Commission, Washington, DC 20555-0001...

Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation.

PubMed

Vedantam, Gayatri; Knopf, Sarah; Hecht, David W

2006-01-01

Tn5520 is the smallest known bacterial mobilizable transposon and was isolated from an antibiotic resistant Bacteroides fragilis clinical isolate. When a conjugation apparatus is provided in trans, Tn5520 is mobilized (transferred) efficiently within, and from, both Bacteroides spp. and Escherichia coli. Only two genes are present on Tn5520; one encodes an integrase, and the other a multifunctional mobilization (Mob) protein BmpH. BmpH is essential for Tn5520 mobility. The focus of this study was to identify the Tn5520 origin of conjugative transfer (oriT) and to study BmpH-oriT binding. We delimited the functional Tn5520 oriT to a 71 bp sequence upstream of the bmpH gene. A plasmid vector harbouring this minimal 71 bp oriT was mobilized at the same frequency as that of intact Tn5520. The minimal oriT contains one 17 bp inverted repeat (IR) sequence. We constructed and tested multiple IR mutants and showed that the IR was essential in its entirety for mobilization. A nick site sequence (5'-GCTAC-3') was also identified within the minimal oriT; this sequence resembled nick sites found in plasmids of Gram positive origin. We further showed that mutation of a highly conserved GC dinucleotide in the nick site sequence completely abolished mobilization. We also purified BmpH and showed that it specifically bound a Tn5520 oriT fragment in electrophoretic mobility shift assays. We also identified non-nick site sequences within the minimal oriT that were essential for mobilization. We hypothesize that transposon-based single Mob protein systems may contribute to efficient gene dissemination from Bacteroides spp., because fewer DNA processing proteins are required for relaxosome formation.

Morphological and functional diversity of ectomycorrhizal fungi on Roan Mountain (NC/TN)

Treesearch

Claire Bird; Coleman McCleneghan

2005-01-01

A comparison of ectomycorrhizal morphotypes and hypogeous fungi (truffles and false-truffles) in northern hardwood and spruce-fir forests on Roan Mountain (NC/TN) was performed to increase our knowledge of the fungal communities in the Southern Appalachian high elevation forests. These forests are home to an endangered subspecies and mycophagist, the Carolina northern...

Recruitment of CRISPR-Cas systems by Tn7-like transposons.

PubMed

Peters, Joseph E; Makarova, Kira S; Shmakov, Sergey; Koonin, Eugene V

2017-08-29

A survey of bacterial and archaeal genomes shows that many Tn7-like transposons contain minimal type I-F CRISPR-Cas systems that consist of fused cas8f and cas5f , cas7f , and cas6f genes and a short CRISPR array. Several small groups of Tn7-like transposons encompass similarly truncated type I-B CRISPR-Cas. This minimal gene complement of the transposon-associated CRISPR-Cas systems implies that they are competent for pre-CRISPR RNA (precrRNA) processing yielding mature crRNAs and target binding but not target cleavage that is required for interference. Phylogenetic analysis demonstrates that evolution of the CRISPR-Cas-containing transposons included a single, ancestral capture of a type I-F locus and two independent instances of type I-B loci capture. We show that the transposon-associated CRISPR arrays contain spacers homologous to plasmid and temperate phage sequences and, in some cases, chromosomal sequences adjacent to the transposon. We hypothesize that the transposon-encoded CRISPR-Cas systems generate displacement (R-loops) in the cognate DNA sites, targeting the transposon to these sites and thus facilitating their spread via plasmids and phages. These findings suggest the existence of RNA-guided transposition and fit the guns-for-hire concept whereby mobile genetic elements capture host defense systems and repurpose them for different stages in the life cycle of the element.

76 FR 29284 - Tennessee Disaster #TN-00055

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-20

... Counties: Benton, Carroll, Crockett, Dyer, Gibson, Henderson, Henry, Houston, Lake, Lauderdale, Madison.... (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator...

An efficient (t,n) threshold quantum secret sharing without entanglement

NASA Astrophysics Data System (ADS)

Qin, Huawang; Dai, Yuewei

2016-04-01

An efficient (t,n) threshold quantum secret sharing (QSS) scheme is proposed. In our scheme, the Hash function is used to check the eavesdropping, and no particles need to be published. So the utilization efficiency of the particles is real 100%. No entanglement is used in our scheme. The dealer uses the single particles to encode the secret information, and the participants get the secret through measuring the single particles. Compared to the existing schemes, our scheme is simpler and more efficient.

Different Variations of Néel Temperature TN and Kondo Temperature TK in the Alloy System Ce(Ru1-xOsx)2Al10 under Uniaxial Pressure

NASA Astrophysics Data System (ADS)

Takeuchi, Takashi; Hayashi, Kyosuke; Umeo, Kazunori; Takabatake, Toshiro

2018-05-01

We report magnetic, transport, and specific-heat measurements for single crystals of the antiferromagnetic (AFM) Kondo semiconductor alloy series Ce(Ru1-xOsx)2Al10 (0 ≤ x ≤ 1), which crystallize into an orthorhombic structure. The specific-heat and resistivity data show that the isoelectronic substitution does not damage the hybridization gap or the AFM transition. The Kondo temperature TK increases linearly with x, whereas the Néel temperature TN exhibits a maximum value of 29.2 K for x = 0.71. Under increasing uniaxial pressure P || a, TN increases for x = 0 but decreases for x = 1, while TK increases in the entire range of x. Under P || b, in contrast, TN increases steadily in the whole range of x while TK remains unchanged for each x. The strongly anisotropic change in TN indicates the presence of another mechanism to enhance TN in this system in addition to the anisotropic hybridization of the 4f state with conduction bands.

1. GENERAL VIEW OF CROSS ROW BUILDING (in background), LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. GENERAL VIEW OF CROSS ROW BUILDING (in background), LOOKING SOUTHWEST. The building at right is Brick Row (Old Beersheba Inn, Brick Row, HABS No. TN-54 B) - Old Beersheba Inn, Cross Row (Boarding Cabin), Armsfield Avenue, Beersheba Springs, Grundy County, TN

Enhanced nitrogen removal from piggery wastewater with high NH4+ and low COD/TN ratio in a novel upflow microaerobic biofilm reactor.

PubMed

Meng, Jia; Li, Jiuling; Li, Jianzheng; Antwi, Philip; Deng, Kaiwen; Nan, Jun; Xu, Pianpian

2018-02-01

To enhance nutrient removal more cost-efficiently in microaerobic process treating piggery wastewater characterized by high ammonium (NH 4 + -N) and low chemical oxygen demand (COD) to total nitrogen (TN) ratio, a novel upflow microaerobic biofilm reactor (UMBR) was constructed and the efficiency in nutrient removal was evaluated with various influent COD/TN ratios and reflux ratios. The results showed that the biofilm on the carriers had increased the biomass in the UMBR and enhanced the enrichment of slow-growth-rate bacteria such as nitrifiers, denitrifiers and anammox bacteria. The packed bed allowed the microaerobic biofilm process perform well at a low reflux ratio of 35 with a NH 4 + -N and TN removal as high as 93.1% and 89.9%, respectively. Compared with the previously developed upflow microaerobic sludge reactor, the UMBR had not changed the dominant anammox approach to nitrogen removal, but was more cost-efficiently in treating organic wastewater with high NH 4 + -N and low COD/TN ratio. Copyright © 2017 Elsevier Ltd. All rights reserved.

36. Close view of the east elevation looking north to ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

36. Close view of the east elevation looking north to south beginning at the northeast corner to show square corner notches and protruding ceiling joists (duplicate of HABS no. TN-52-B-17) - The Hermitage, East Cabin, 4580 Rachel's Lane, Hermitage, Davidson County, TN

78 FR 6257 - Proposed Amendment of Class C Airspace; Nashville International Airport, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-30

...-0031; Airspace Docket No. 12-AWA-7] RIN 2120-AA66 Proposed Amendment of Class C Airspace; Nashville... rulemaking (NPRM). SUMMARY: This action proposes to modify the Nashville International Airport, TN, Class C... around an airport that is now permanently closed. The FAA is proposing this action to return the Class C...

Diagnostic value of analysis of H-FABP, NT-proBNP, and cTnI in heart function in children with congenital heart disease and pneumonia.

PubMed

Zhou, F-J; Zhou, C-Y; Tian, Y-J; Xiao, A-J; Li, P-L; Wang, Y-H; Jia, J-W

2014-01-01

To analyze the expression of heart-fatty acid-binding protein (H-FABP), N-terminal pro-brain natriuretic peptide (NT-proBNP), and cTnI in children with congenital heart disease (CHD) and pneumonia, and evaluate its diagnostic value in heart failure (HF). The levels of serum H-FABP, NT-proBNP, and cardiac troponin 1 (cTnI) were measured by immunoassays in 22 children with CHD, pneumonia, and HF (group I), 25 children with CHD and pneumonia (group II), and 25 healthy children without CHD or pneumonia (control group). The concentration and positive rate of serum H-FABP, NT-proBNP, and cTnI were significantly higher in group I than those in group II. Compared to control group, these indexes were increased in both group I and group II. There were statistical significant differences in the positive rate of NT-proBNP and cTnI but not H-FABP between groups of patients with different classes of heart function. The levels of H-FABP, NT-proBNP, and cTnI were correlated with heart function, and can be used for the diagnosis of early-stage HF in children with CHD.

[Improvement of butanol production by Escherichia coli via Tn5 transposon mediated mutagenesis].

PubMed

Lin, Zhao; Dong, Hongjun; Li, Yin

2015-12-01

For engineering an efficient butanol-producing Escherichia coli strain, many efforts have been paid on the known genes or pathways based on current knowledge. However, many genes in the genome could also contribute to butanol production in an unexpected way. In this work, we used Tn5 transposon to construct a mutant library including 1 196 strains in a previously engineered butanol-producing E. coli strain. To screen the strains with improved titer of butanol production, we developed a high-throughput method for pyruvate detection based on dinitrophenylhydrazine reaction using 96-well microplate reader, because pyruvate is the precursor of butanol and its concentration is inversely correlated with butanol in the fermentation broth. Using this method, we successfully screened three mutants with increased butanol titer. The insertion sites of Tn5 transposon was in the ORFs of pykA, tdk, and cadC by inverse PCR and sequencing. These found genes would be efficient targets for further strain improvement. And the genome scanning strategy described here will be helpful for other microbial cell factory construction.

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT) Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China

PubMed Central

Xiong, Yao; Liu, Bian; Hao, Zilong; Tao, Wendan; Liu, Ming

2016-01-01

Background and Objective Elevated levels of high sensitivity cardiac troponin T (hs-cTnT) occur in a substantial proportion of patients with acute ischemic stroke (AIS) and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT) or prognosis in AIS patients with rheumatic heart disease (RHD) remains unclear. Methods Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels(≥14ng/L) and patients with normal hs-cTnT levels (<14ng/L). Results The final analysis involved 84 patients (31 males; mean age, 61.6±12.2years), of whom serum hs-cTnT levels were elevated in 58.3%. Renal impairment was independently associated with elevated hs-cTnT levels (OR 4.184, 95%CI 1.17 to 15.01, P = 0.028), and patients with elevated hs-cTnT levels were at significantly higher risk of HT, 3-month mortality and 3-month disability/mortality (all P≤0.029). After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels. Conclusion Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies. PMID:26849554

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT) Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China.

PubMed

Liu, Junfeng; Wang, Deren; Xiong, Yao; Liu, Bian; Hao, Zilong; Tao, Wendan; Liu, Ming

2016-01-01

Elevated levels of high sensitivity cardiac troponin T (hs-cTnT) occur in a substantial proportion of patients with acute ischemic stroke (AIS) and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT) or prognosis in AIS patients with rheumatic heart disease (RHD) remains unclear. Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within 1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels (≥14 ng/L) and patients with normal hs-cTnT levels (<14 ng/L). The final analysis involved 84 patients (31 males; mean age, 61.6±12.2 years), of whom serum hs-cTnT levels were elevated in 58.3%. Renal impairment was independently associated with elevated hs-cTnT levels (OR 4.184, 95%CI 1.17 to 15.01, P = 0.028), and patients with elevated hs-cTnT levels were at significantly higher risk of HT, 3-month mortality and 3-month disability/mortality (all P≤0.029). After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels. Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies.

An unusual Tn21-like transposon containing an ars operon is present in highly arsenic-resistant strains of the biomining bacterium Acidithiobacillus caldus.

PubMed

Tuffin, I Marla; de Groot, Peter; Deane, Shelly M; Rawlings, Douglas E

2005-09-01

A transposon, TnAtcArs, that carries a set of arsenic-resistance genes was isolated from a strain of the moderately thermophilic, sulfur-oxidizing, biomining bacterium Acidithiobacillus caldus. This strain originated from a commercial plant used for the bio-oxidation of gold-bearing arsenopyrite concentrates. Continuous selection for arsenic resistance over many years had made the bacterium resistant to high concentrations of arsenic. Sequence analysis indicated that TnAtcArs is 12 444 bp in length and has 40 bp terminal inverted repeat sequences and divergently transcribed resolvase and transposase genes that are related to the Tn21-transposon subfamily. A series of genes consisting of arsR, two tandem copies of arsA and arsD, two ORFs (7 and 8) and arsB is situated between the resolvase and transposase genes. Although some commercial strains of At. caldus contained the arsDA duplication, when transformed into Escherichia coli, the arsDA duplication was unstable and was frequently lost during cultivation or if a plasmid containing TnAtcArs was conjugated into a recipient strain. TnAtcArs conferred resistance to arsenite and arsenate upon E. coli cells. Deletion of one copy of arsDA had no noticeable effect on resistance to arsenite or arsenate in E. coli. ORFs 7 and 8 had clear sequence similarity to an NADH oxidase and a CBS-domain-containing protein, respectively, but their deletion did not affect resistance to arsenite or arsenate in E. coli. TnAtcArs was actively transposed in E. coli, but no increase in transposition frequency in the presence of arsenic was detected. Northern hybridization and reporter gene studies indicated that although ArsR regulated the 10 kb operon containing the arsenic-resistance genes in response to arsenic, ArsR had no effect on the regulation of genes associated with transposition activity.

Efficient transformation of sucrose into high pullulan concentrations by Aureobasidium melanogenum TN1-2 isolated from a natural honey.

PubMed

Jiang, Hong; Xue, Si-Jia; Li, Yan-Feng; Liu, Guang-Lei; Chi, Zhen-Ming; Hu, Zhong; Chi, Zhe

2018-08-15

A very high pullulan producing yeast-like fungus, Aureobasidium melanogenum TN1-2 isolated from a natural honey was found to be able to produce 97.0 g/L of pullulan from 140.0 g/L sucrose at a flask level while it could yield 114.0 g/L of pullulan within 134 h during a 10-liter fermentation, the yield was 0.81 g/g and the productivity was 0.86 g/L/h. The high ability to biosynthesize pullulan by this yeast-like fungal strain TN1-2 was related to high glucosyltransferase activity, high phosphofructo-2-kinase activity, high content of its cellular glycerol and low glucose repressor. The Mw of the produced pullulan was 1.42 × 10 5  g/mol. The low Mw may be due to the high α-amylase, glucoamylase and isopullulanase activities. The intracellular level of trehalose had no influence on high pullulan production by the yeast-like fungal strain TN1-2. Copyright © 2018. Published by Elsevier Ltd.

75 FR 27009 - Tennessee Disaster Number TN-00039

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-13

..., Houston, Madison, Obion. Contiguous Counties: (Economic Injury Loans Only): Tennessee: Henry, Weakley... remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera...

76 FR 33395 - Tennessee Disaster Number TN-00051

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-08

... Counties: (Physical Damage and Economic Injury Loans): Carroll, Crockett, Hardin, Henry, Madison... remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera...

77 FR 15995 - Foreign-Trade Zone 148-Knoxville, TN; Application for Reorganization Under Alternative Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-19

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 16-2012] Foreign-Trade Zone 148--Knoxville, TN; Application for Reorganization Under Alternative Site Framework An application has been... and adjacent to the Knoxville U.S. Customs and Border Protection port of entry. The applicant is...

pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matsumoto, Yasuyuki; Zhang, Qing; Akita, Kaoru

2012-03-02

Highlights: Black-Right-Pointing-Pointer ppGalNAc-T13 was up-regulated in high metastatic sublines of Lewis lung cancer. Black-Right-Pointing-Pointer ppGalNAc-T13 expression enhanced cell invasion activity in low metastatic sublines. Black-Right-Pointing-Pointer Trimeric Tn antigen was induced in the transfectant cells of ppGalNAc-T13 cDNA. Black-Right-Pointing-Pointer A major protein carrying trimeric Tn structure was identified as Syndecan-1. Black-Right-Pointing-Pointer Silencing of ppGalNAc-T13 resulted in the reduction of invasion and of metastasis.. -- Abstract: In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. Thesemore » sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated

EPA Announces the Availability of the Removal Administrative Record for the American Drum and Pallet Site in Memphis, Shelby County, Tennessee, the Daily News - Memphis, TN

EPA Pesticide Factsheets

Contains proof of publication of announcement of availability of the removal administrative record for the American Drum and Pallet Site in Memphis, Shelby County, Tennessee, in the Daily News newspaper Region ID: 04 DocID: 10547927, DocDate: 12-24-2007

Tn5253 family integrative and conjugative elements carrying mef(I) and catQ determinants in Streptococcus pneumoniae and Streptococcus pyogenes.

PubMed

Mingoia, Marina; Morici, Eleonora; Morroni, Gianluca; Giovanetti, Eleonora; Del Grosso, Maria; Pantosti, Annalisa; Varaldo, Pietro E

2014-10-01

The linkage between the macrolide efflux gene mef(I) and the chloramphenicol inactivation gene catQ was first described in Streptococcus pneumoniae (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in Streptococcus pyogenes (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253 family integrative and conjugative elements (ICEs). The ICE from S. pneumoniae (ICESpn529IQ) was sequenced, whereas the ICEs from S. pyogenes (ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share the intSp 23FST81 integrase gene and an identical Tn916 fragment, whereas ICESpy005IQ has int5252 and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (intSp 23FST81 or int5252). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation, mef(I) and catQ were cotransferred at a high frequency from S. pyogenes Spy005 and at very low frequencies from S. pneumoniae Spn529 and S. pyogenes Spy029. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

75 FR 63177 - Availability of FY 09 Grantee Performance Evaluation Reports for the Eight States of EPA Region 4...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-14

...-year evaluations of eight state air pollution control programs (Alabama Department of Environmental... Management, FL; Louisville Metro Air Pollution Control District, KY; Forsyth County Environmental Affairs... Regional Air Quality Agency, NC; Chattanooga-Hamilton County Air Pollution Control Bureau, TN; Memphis...

FAA Air Traffic Activity: FY 1990. Addendum.

DTIC Science & Technology

1990-01-01

SONOMA COUNTY (STS) N ITINERANT OPERATIONS ................................................................. 107516 910 9673 96240 693 LOCAL OPERATIONS...TX N 282 9 SANTA ROSA SONOMA COUNTY .............. CA N 213 910 MUNCIE DELAWARE CNTY .................. IN N 283 8 PAGO PAGO...INTERNATIONAL .................. TX M 50 97498 KNOXVILLE MCGHEE TYSON .................. TN S 130 62271 SANTA ROSA SONOMA COUNTY .............. CA N 51 96240

76 FR 53115 - Foreign-Trade Zone 77-Memphis, TN; Application for Temporary/Interim Manufacturing Authority...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-25

..., TN; Application for Temporary/ Interim Manufacturing Authority; Flextronics Logistics USA, Inc. (Cell... authority to produce cell phones/mobile handsets kits (HTSUS 8517.12, HTSUS 8517.62, HTSUS 8517.69, duty... cell phone/mobile handset kits (duty free) for the foreign inputs noted above. In accordance with the...

Low-temperature thermochronology of the Laramide Ranges and eastward translation of shortening in the Sevier Belt, Wyoming, Utah and Montana

NASA Astrophysics Data System (ADS)

Peyton, Sara Lynn

This dissertation contains two studies that use very different techniques to investigate the Mesozoic and Cenozoic tectonics of the western USA. The first study investigates shortening in the Sevier thrust belt of northeast Utah and southwest Wyoming using cross sections and seismic reflection data. The second study investigates the low-temperature thermochronology of the Laramide Ranges using apatite (U-Th)/He dating. We used cross sections and seismic reflection data to investigate bed length discrepancies within the hanging wall of the Absaroka thrust in the Sevier thrust belt of northeast Utah and southwest Wyoming. Restoration of cross sections suggests that there was ˜8-14 km of pre-Absaroka-thrust shortening above the Jurassic Preuss salt detachment, but not below it, in the hanging wall of the Absaroka thrust. Reflection seismic data over the hanging wall of the Crawford thrust show that the Crawford thrust is not offset along the Preuss salt detachment, indicating that the additional shortening on the Absaroka plate was transferred east before main movement on the Crawford thrust. Although early displacement on the Crawford thrust cannot be ruled out as the cause of the extra shortening, surface and subsurface geology suggests slip from the western thrust system (Willard and Lost Creek thrusts) was transferred several tens of kilometers east along the Jurassic Preuss salt detachment between ˜102-90 Ma, to the future location of the Absaroka thrust hanging wall. The lack of deformation of the Crawford thrust on the seismic data, along with shortening and extension estimates from cross sections, also indicate that the magnitude of Paleocene and post-early Eocene shortening on the Medicine Butte thrust was essentially offset by subsequent extension on the middle Eocene to late Oligocene Almy-Acocks normal-fault system. For the second study in this dissertation, we dated 91 borehole and surface samples from Laramide-age, basement-cored uplifts of the Rocky

The L‐type Ca2+ channel facilitates abnormal metabolic activity in the cTnI‐G203S mouse model of hypertrophic cardiomyopathy

PubMed Central

Viola, Helena; Johnstone, Victoria; Cserne Szappanos, Henrietta; Richman, Tara; Tsoutsman, Tatiana; Filipovska, Aleksandra; Semsarian, Christopher

2016-01-01

Key points Genetic mutations in cardiac troponin I (cTnI) are associated with development of hypertrophic cardiomyopathy characterized by myocyte remodelling, disorganization of cytoskeletal proteins and altered energy metabolism.The L‐type Ca2+ channel is the main route for calcium influx and is crucial to cardiac excitation and contraction. The channel also regulates mitochondrial function in the heart by a functional communication between the channel and mitochondria via the cytoskeletal network.We find that L‐type Ca2+ channel kinetics are altered in cTnI‐G203S cardiac myocytes and that activation of the channel causes a significantly greater increase in mitochondrial membrane potential and metabolic activity in cTnI‐G203S cardiac myocytes.These responses occur as a result of impaired communication between the L‐type Ca2+ channel and cytoskeletal protein F‐actin, involving decreased movement of actin–myosin and block of the mitochondrial voltage‐dependent anion channel, resulting in a ‘hypermetabolic’ mitochondrial state.We propose that L‐type Ca2+ channel antagonists, such as diltiazem, might be effective in reducing the cardiomyopathy by normalizing mitochondrial metabolic activity. Abstract Genetic mutations in cardiac troponin I (cTnI) account for 5% of families with hypertrophic cardiomyopathy. Hypertrophic cardiomyopathy is associated with disorganization of cytoskeletal proteins and altered energy metabolism. The L‐type Ca2+ channel (ICa‐L) plays an important role in regulating mitochondrial function. This involves a functional communication between the channel and mitochondria via the cytoskeletal network. We investigate the role of ICa‐L in regulating mitochondrial function in 25‐ to 30‐week‐old cardiomyopathic mice expressing the human disease‐causing mutation Gly203Ser in cTnI (cTnI‐G203S). The inactivation rate of ICa‐L is significantly faster in cTnI‐G203S myocytes [cTnI‐G203S: τ1 = 40.68 ± 3.22, n

Spatiotemporal Co-occurrence of Flanders and West Nile Viruses Within Culex Populations in Shelby County, Tennessee.

PubMed

Lucero, D E; Carlson, T C; Delisle, J; Poindexter, S; Jones, T F; Moncayo, A C

2016-05-01

West Nile virus (WNV) and Flanders virus (FLAV) can cocirculate in Culex mosquitoes in parts of North America. A large dataset of mosquito pools tested for WNV and FLAV was queried to understand the spatiotemporal relationship between these two viruses in Shelby County, TN. We found strong evidence of global clustering (i.e., spatial autocorrelation) and overlapping of local clustering (i.e., Hot Spots based on Getis Ord Gi*) of maximum likelihood estimates (MLE) of infection rates (IR) during 2008-2013. Temporally, FLAV emerges and peaks on average 10.2 wk prior to WNV based on IR. Higher levels of WNV IR were detected within 3,000 m of FLAV-positive pool buffers than outside these buffers. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

On the almost everywhere convergence of the eigenfunction expansions from Liouville classes L_1^\\alpha ({T^N})

NASA Astrophysics Data System (ADS)

Ahmedov, Anvarjon; Materneh, Ehab; Zainuddin, Hishamuddin

2017-09-01

The relevance of waves in quantum mechanics naturally implies that the decomposition of arbitrary wave packets in terms of monochromatic waves plays an important role in applications of the theory. When eigenfunction expansions does not converge, then the expansions of the functions with certain smoothness should be considered. Such functions gained prominence primarily through their application in quantum mechanics. In this work we study the almost everywhere convergence of the eigenfunction expansions from Liouville classes L_p^α ({T^N}), related to the self-adjoint extension of the Laplace operator in torus TN . The sufficient conditions for summability is obtained using the modified Poisson formula. Isomorphism properties of the elliptic differential operators is applied in order to obtain estimation for the Fourier series of the functions from the classes of Liouville L_p^α .

Distribution host status and potential sources of resistance to Vittatidera zeaphila

USDA-ARS?s Scientific Manuscript database

Vittatidera zeaphila was described from stunted Zea mays (corn) roots collected in northwestern Tennessee (Obion County) in 2006. Similar cyst specimens had previously been collected in 1978 from Lauderdale County, TN, on Eleusine indica (goosegrass). Comparison of the 1978 specimens deposited in t...

75 FR 28302 - American Food and Vending Spring Hill, TN; Notice of Negative Determination Regarding Application...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-20

... Spring Hill, TN; Notice of Negative Determination Regarding Application for Reconsideration By... negative determination applicable to workers and former workers at American Food and Vending, Spring Hill... eligible for TAA because they are service workers who provided services to General Motors, Spring Hill...

Environmental Assessment: Johnson County Road F-20 Bypass, Johnson County, Iowa.

DTIC Science & Technology

1986-08-01

D-A184 328 ENVIRONMENTAL ASSESSMENT- JOHNSON COUNTY ROAD F-20 vil BYPASS JOHNSON COUNTY’ IOWA (U) ARMY ENGINEER DISTRICT ROCK ISLAND IL AUG 86...BYPASS JOHNSON COUNTY, IOWA DTIC SELECTEI AUG 24 198D AUGUST 1986 -’"Thn; c>, .. i ’, been p ore i Icr publj rJlxaje aid sale; ii ,,. I distibu on is...2004 19 EPLY TO ATTENTION OP NCRPD-E ENVIRONMENTAL ASSE SSMENT JOHNSON COUNTY ROAD F-20 BYPASS JOHNSON COUNTY, IOWA Acce3siofl For NTIS MR& QUA~rTY

75 FR 74002 - Foreign-Trade Zone 148-Knoxville, TN, Application for Subzone Toho, Tenax America, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-30

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 57-2010] Foreign-Trade Zone 148--Knoxville, TN, Application for Subzone Toho, Tenax America, Inc., Extension of Comment Period The comment period for the application for subzone status at the Toho Tenax America, Inc., facility in Rockwood...

A TALE of transposition: Tn3-like transposons play a major role in the spread of pathogenicity determinants of Xanthomonas citri and other xanthomonads.

PubMed

Ferreira, Rafael Marini; de Oliveira, Amanda Carolina P; Moreira, Leandro M; Belasque, José; Gourbeyre, Edith; Siguier, Patricia; Ferro, Maria Inês T; Ferro, Jesus A; Chandler, Michael; Varani, Alessandro M

2015-02-17

Members of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomonas citri plasmid pXAC64. These are flanked by short inverted repeats (IRs). The region was identified as a transposon, TnXax1, with typical Tn3 family features, including a transposase and two recombination genes. Two 14-bp palindromic sequences within a 193-bp potential resolution site occur between the recombination genes. Additional derivatives carrying different T3SEs and other passenger genes occur in different Xanthomonas species. The T3SEs include transcription activator-like effectors (TALEs). Certain TALEs are flanked by the same IRs as found in TnXax1 to form mobile insertion cassettes (MICs), suggesting that they may be transmitted horizontally. A significant number of MICs carrying other passenger genes (including a number of TALE genes) were also identified, flanked by the same TnXax1 IRs and delimited by 5-bp target site duplications. We conclude that a large fraction of T3SEs, including individual TALEs and potential pathogenicity determinants, have spread by transposition and that TnXax1, which exhibits all of the essential characteristics of a functional transposon, may be involved in driving MIC transposition. We also propose that TALE genes may diversify by fork slippage during the replicative Tn3 family transposition. These mechanisms may play a crucial role in the emergence of Xanthomonas pathogenicity. Xanthomonas genomes carry many insertion sequences (IS) and transposons, which play an important role in their evolution and architecture. This study reveals a key relationship between transposons and pathogenicity determinants in

RADIOCARBON MEASUREMENT OF THE BIOGENIC CONTRIBUTION TO SUMMERTIME PM 2.5 AMBIENT AEROSOL IN NASHVILLE, TN

EPA Science Inventory

Radiocarbon (14C) measurements performed on PM-2.5 samples collected near Nashville, TN from June 21 to July 13, 1999, showed high levels of modern carbon, ranging from 56 to 80% of the total carbon in the samples. Radiocarbon measurements performed on dichloromethane extracts of...

Asia-Pacific consensus statement on the optimal use of high-sensitivity troponin assays in acute coronary syndromes diagnosis: focus on hs-TnI

PubMed Central

Tan, Jack Wei Chieh; Lam, Carolyn S P; Kasim, Sazzli Shahlan; Aw, Tar Choon; Abanilla, Joel M; Chang, Wei-Ting; Dang, Van Phuoc; Iboleon-Dy, Maria; Mumpuni, Sari Sri; Phommintikul, Arintaya; Ta, Manh Cuong; Topipat, Punkiat; Yiu, Kai Hang; Cullen, Louise

2017-01-01

Objective High-sensitivity troponin (hs-Tn) assays need to be applied appropriately to improve diagnosis and patient outcomes in acute coronary syndromes (ACS). Methods Experts from Asia Pacific convened in 2015 to provide data-driven consensus-based, region-specific recommendations and develop an algorithm for the appropriate incorporation of this assay into the ACS assessment and treatment pathway. Results Nine recommendations were developed by the expert panel: (1) troponin is the preferred cardiac biomarker for diagnostic assessment of ACS and is indicated for patients with symptoms of possible ACS; (2) hs-Tn assays are recommended; (3) serial testing is required for all patients; (4) testing should be performed at presentation and 3 hours later; (5) gender-specific cut-off values should be used for hs-Tn I assays; (6) hs-Tn I level >10 times the upper limit of normal should be considered to ‘rule in’ a diagnosis of ACS; (7) dynamic change >50% in hs-Tn I level from presentation to 3-hour retest identifies patients at high risk for ACS; (8) where only point-of-care testing is available, patients with elevated readings should be considered at high risk, while patients with low/undetectable readings should be retested after 6 hours or sent for laboratory testing and (9) regular education on the appropriate use of troponin tests is essential. Conclusions We propose an algorithm that will potentially reduce delays in discharge by the accurate ‘rule out’ of non-ACS patients within 3 hours. Appropriate research should be undertaken to ensure the efficacy and safety of the algorithm in clinical practice, with the long-term goal of improvement of care of patients with ACS in Asia Pacific. PMID:28466882

Heart-type fatty acid-binding protein (H-FABP) and highly sensitive troponin T (hsTnT) as markers of myocardial injury and cardiovascular events in elective percutaneous coronary intervention (PCI).

PubMed

Connolly, M; Shand, J; Kinnin, M; Menown, I; Kurth, M J; Lamont, J; Mc Eneaney, D

2018-01-01

Type 4a myocardial infarction (MI) occurs when myocardial injury is combined with either symptoms suggestive of myocardial ischaemia, new left bundle branch block, angiographic loss of patency of a major artery or imaging suggestive of new loss of myocardium. Myocardial injury is defined as a rise of >5 x 99th upper reference limit (URL) of 14 ng/l (i.e. >70 ng/l) for highly sensitive troponin T (hsTnT) at 6 h if hsTnT was normal at baseline or >20% rise from 0 to 6 h if hsTnT was >14 ng/l at baseline. To assess the prognostic value of biomarkers of myocardial injury following elective percutaneous coronary intervention (PCI). A cohort of 209 patients were included of whom 144 (68.9%) were male, mean age was 68.8 years, 28 (13.4%) were smokers, 31 (14.8%) were diabetic, 199 (95.2%) had hypercholesterolaemia and 138 (66.0%) had hypertension. We evaluated hsTnT, heart-type fatty acid-binding protein (H-FABP), troponin I (TnI), creatine kinase MB type (CKMB), myoglobin, glycogen phosphorylase BB (GPBB) and carbonic anhydrase III (CA III) at 0, 4, 6 and 24 h following elective PCI. Patients were followed up at 1 year to assess for major adverse clinical events (MACE). Myocardial injury was observed in 37 (17.7%) patients. Median hsTnT/H-FABP at 4 h were most predictive. MACE was noted in 6 (2.9%) patients, 3 had type 4a MI post PCI, P = 0.036. Median 4 h hsTnT/H-FABP were most predictive of myocardial injury following PCI. H-FABP and hsTnT were predictive of MACE. © The Author 2017. Published by Oxford University Press on behalf of the Association of Physicians. All rights reserved. For Permissions, please email: journals.permissions@oup.com

'Test n Treat (TnT)'- Rapid testing and same-day, on-site treatment to reduce rates of chlamydia in sexually active further education college students: study protocol for a cluster randomised feasibility trial.

PubMed

Kerry-Barnard, Sarah; Fleming, Charlotte; Reid, Fiona; Phillips, Rachel; Drennan, Vari M; Adams, Elisabeth J; Majewska, Wendy; Balendra, Anjella; Harding-Esch, Emma; Cousins, Emma; Tariq Sadiq, S; Oakeshott, Pippa

2018-06-05

Sexually active young people attending London further education (FE) colleges have high rates of chlamydia, but screening rates are low. We will conduct a cluster randomised feasibility trial of frequent, rapid, on-site chlamydia testing and same-day treatment (Test and Treat (TnT)) in six FE colleges (with parallel qualitative and economic assessments) to assess the feasibility of conducting a future trial to investigate if TnT reduces chlamydia rates. We will recruit 80 sexually active students aged 16-24 years from public areas at each of six colleges. All participants (total n = 480) will be asked to provide samples (urine for males, self-taken vaginal swabs for females) and complete questionnaires on sexual lifestyle and healthcare use at baseline and after 7 months. Participants will be informed that baseline samples will not be tested for 7 months and be advised to get screened separately. Colleges will be randomly allocated to the intervention (TnT) or the control group (no TnT). One and 4 months after recruitment, participants at each intervention college (n = 3) will be texted and invited for on-site chlamydia tests using the 90-min Cepheid GeneXpert system. Students with positive results will be asked to see a visiting nurse health adviser for same-day treatment and partner notification, (backed by genitourinary medicine follow-up). Participants in control colleges (n = 3) will receive 'thank you' texts 1 and 4 months after recruitment. Seven months after recruitment, participants from both groups will be invited to complete questionnaires and provide samples for TnT. All samples will be tested, and same-day treatment offered to students with positive results. Acceptability of TnT will be assessed by qualitative interviews of purposively sampled students (n = 30) and college staff (n = 12). We will collect data on costs of TnT and usual healthcare. Findings will provide key values to inform feasibility, sample size and timescales of a

Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

PubMed Central

Gunderson, Leonard L.; Moughan, Jennifer; Ajani, Jaffer A.; Pedersen, John E.; Winter, Kathryn A.; Benson, Al B.; Thomas, Charles R.; Mayer, Robert J.; Haddock, Michael G.; Rich, Tyvin A.; Willett, Christopher G.

2013-01-01

Purpose The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the log–rank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray’s test. Results Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories. PMID:24035327

77 FR 73978 - Foreign-Trade Zone 148-Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-12

...--Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing Authority), Opening of Comment Period on... manufacture carbon fiber for export and oxidized polyacrylonitrile fiber (Board Order 1868, 77 FR 69435, 11/19/2012). Board Order 1868 did not include authority to manufacture carbon fiber for the U.S. market; the...

7. Photograph of a line drawing. 'PART III, SECTION 1, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDING NO. 10, PRODUCER GAS & EXHAUSTER BLDG., PLANT A.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant A, Parts I, II, III. (Nashville, TN: Office of District Engineer, 1944). - Holston Army Ammunition Plant, Producer Gas Plant, Kingsport, Sullivan County, TN

77 FR 3468 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-24

... Energy LLC, Hardee Power Partners Limited, Judith Gap Energy LLC, Invenergy TN LLC, Wolverine Creek... Energy V LLC, Vantage Wind Energy LLC, Beech Ridge Energy LLC, Gratiot County Wind LLC, Stony Creek Energy LLC, Gratiot County Wind II LLC. Description: Notification of Change in Facts Under Market-Based...

75 FR 61696 - Foreign-Trade Zone 148-Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-06

...--Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon Fiber and Oxidized..., requesting special-purpose subzone status for the carbon fiber and oxidized polyacrylonitrile fiber (OPF...)--based carbon fiber and OPF (up to 4,000 metric tons combined annually) for export and the domestic...

Troponin C Mutations Partially Stabilize the Active State of Regulated Actin and Fully Stabilize the Active State When Paired with Δ14 TnT.

PubMed

Baxley, Tamatha; Johnson, Dylan; Pinto, Jose R; Chalovich, Joseph M

2017-06-13

Striated muscle contraction is regulated by the actin-associated proteins tropomyosin and troponin. The extent of activation of myosin ATPase activity is lowest in the absence of both Ca 2+ and activating cross-bridges (i.e., S1-ADP or rigor S1). Binding of activating species of myosin to actin at a saturating Ca 2+ concentration stabilizes the most active state (M state) of the actin-tropomyosin-troponin complex (regulated actin). Ca 2+ binding alone produces partial stabilization of the active state. The extent of stabilization at a saturating Ca 2+ concentration depends on the isoform of the troponin subunits, the phosphorylation state of troponin, and, in the case of cardiac muscle, the presence of hypertrophic cardiomyopathy-producing mutants of troponin T and troponin I. Cardiac dysfunction is also associated with mutations of troponin C (TnC). Troponin C mutants A8V, C84Y, and D145E increase the Ca 2+ sensitivity of ATPase activity. We show that these mutants change the distribution of regulated actin states. The A8V and C84Y TnC mutants decreased the inactive B state distribution slightly at low Ca 2+ concentrations, but the D145E mutants had no effect on that state. All TnC mutants increased the level of the active M state compared to that of the wild type, at a saturating Ca 2+ concentration. Troponin complexes that contained two mutations that stabilize the active M state, A8V TnC and Δ14 TnT, appeared to be completely in the active state in the presence of only Ca 2+ . Because Ca 2+ gives full activation, in this situation, troponin must be capable of positioning tropomyosin in the active M state without the need for rigor myosin binding.

Sonoma County Office of Education Computer Education Plan. County Level Plans.

ERIC Educational Resources Information Center

Malone, Greg

1986-01-01

This plan describes the educational computing and computer literacy program to be implemented by the schools in Sonoma County, California. Topics covered include the roles, responsibilities, and procedures of the county-wide computer committee; the goals of computer education in the county schools; the results of a needs assessment study; a 3-year…

Abrasive wear of Hilong BoTN hardfacings

NASA Astrophysics Data System (ADS)

Fedorova, L.; Fedorov, S.; Sadovnikov, A.; Ivanova, Y.; Voronina, M.

2018-02-01

The spread of steels, which are used to produce locks of steel drill pipes, adversely affects their wear resistance, which, in combination with low hardness of HV 2400 ... 2800 MPa as well as of the thread of screw, results in low wear resistance and the need for their reconstruction at the pipe control shop. An efficient way of improving the quality of drill pipe jonts is to hard-face them by the outside diameter with wear-resistant materials (hardbanding). One of the companies engaged in the development of hardfacing materials and hardbanding is Hilong (China) with weld seams of the brand BoTn. According to the results of the studies the following conclusion can be made: hardfacing increases the durability of the hardware, contributing to an increase in wear resistance of locks of DP under the conditions of abrasive action of aggressive geological formations; the usage of DP without wear-resistant weld seams is impermissible, because their further operation, as part of the drill-stem, can lead to emergency consequences; application of the pipes with the hardfacing collars together with the collars without hardfacing, due to varying degree of wear of jonts in the drill-stem, is also impermissible.

78 FR 54168 - Special Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-03

... local regulation will not have a significant economic impact on a substantial number of small entities... Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary special local...

Implications for future activity of Grímsvötn volcano, Iceland, from compositional time series of historical tephra

NASA Astrophysics Data System (ADS)

Carpentier, Marion; Sigmarsson, Olgeir; Larsen, Gudrun

2014-05-01

The nature of future eruptions of active volcanoes is hard to predict. Improved understanding of the past volcanic activity is probably the best way to infer future eruptive scenarios. The most active volcano in Iceland, Grímsvötn, last erupted in 2011 with consequences for habitants living close to the volcano and aviation in the North-Atlantic. In an effort to better understand the magmatic system of the volcano, we have investigated the compositions of 23 selected tephra layers representing the last 8 centuries of volcanic activity at Grímsvötn. The tephra was collected in the ablation area of outlet glaciers from Vatnajökull ice cap. The ice-conserved tephra are less prone to alteration than those exposed in soil sections. Major element analyses are indistinguishable and of quartz-normative tholeiite composition, and Sr and Nd isotope ratios are constant. In contrast, both trace element concentrations (Th range from 0.875 ppm to 1.37 ppm and Ni from 28.5 ppm to 56.6 ppm) in the basalts and Pb isotopes show small but significant variations. The high-precision analyses of Pb isotope ratios allow the identification of tephra samples (3 in total) with more radiogenic ratios than the bulk of the samples. The tephra of constant isotope ratios show linear increase in incompatible element concentrations with time. The rate of increasing concentrations permits exploring possible future scenarios assuming that the magmatic system beneath the volcano follows the established historical evolution. Assuming similar future behaviour of the magma system beneath Grímsvötn volcano, the linear increase in e.g. Th concentration suggests that the volcano is likely to principally produce basalts for the next 500-1000 years. Evolution of water concentration will most likely follow those of incompatible elements with consequent increases in explosiveness of future Grímsvötn eruptions.

77 FR 42761 - Final Environmental Impact Statement for the Oil and Gas Management Plan at Big South Fork...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... conducted in a manner that protects park resources and values, visitor use and experience, and human health... following libraries: McCreary County Public Library 6 North Main Whitley City, KY 42653 Oneida Public Library 290 South Main Street Oneida, TN 37841-2607 Fentress County Public Library 306 South Main Street...

TnSeq of Mycobacterium tuberculosis clinical isolates reveals strain-specific antibiotic liabilities

PubMed Central

Carey, Allison F.; Rock, Jeremy M.; Krieger, Inna V.; Gagneux, Sebastien; Sacchettini, James C.; Fortune, Sarah M.

2018-01-01

Once considered a phenotypically monomorphic bacterium, there is a growing body of work demonstrating heterogeneity among Mycobacterium tuberculosis (Mtb) strains in clinically relevant characteristics, including virulence and response to antibiotics. However, the genetic and molecular basis for most phenotypic differences among Mtb strains remains unknown. To investigate the basis of strain variation in Mtb, we performed genome-wide transposon mutagenesis coupled with next-generation sequencing (TnSeq) for a panel of Mtb clinical isolates and the reference strain H37Rv to compare genetic requirements for in vitro growth across these strains. We developed an analytic approach to identify quantitative differences in genetic requirements between these genetically diverse strains, which vary in genomic structure and gene content. Using this methodology, we found differences between strains in their requirements for genes involved in fundamental cellular processes, including redox homeostasis and central carbon metabolism. Among the genes with differential requirements were katG, which encodes the activator of the first-line antitubercular agent isoniazid, and glcB, which encodes malate synthase, the target of a novel small-molecule inhibitor. Differences among strains in their requirement for katG and glcB predicted differences in their response to these antimicrobial agents. Importantly, these strain-specific differences in antibiotic response could not be predicted by genetic variants identified through whole genome sequencing or by gene expression analysis. Our results provide novel insight into the basis of variation among Mtb strains and demonstrate that TnSeq is a scalable method to predict clinically important phenotypic differences among Mtb strains. PMID:29505613

EnviroAtlas -- Memphis, TN (2012) -- One Meter Resolution Urban Land Cover Data

EPA Pesticide Factsheets

The Memphis, TN EnviroAtlas One Meter-scale Urban Land Cover (MULC) dataset comprises 2,733 km2 around the city of Memphis, surrounding towns, and rural areas. These leaf-on LC data and maps were derived from 1-m pixel, four-band (red, green, blue, and near-infrared) aerial photography acquired from the United States Department of Agriculture (USDA) National Agriculture Imagery Program (NAIP) on four dates in 2012: June 15, June 18, June 21 and June 23, and one date in 2013: July 12. Three separate LiDAR (Light Detection and Ranging) data sets collected on February 19, 2009 00e2?? August 2, 2010, December 1-2, 2011 and January 23-24, 2012 were integrated for Shelby Co., TN, Crittenden Co., AR, and DeSoto Co, MS. Five MULC classes were mapped directly from the NAIP and LiDAR data: Water, Impervious, Soil, Trees, and Grass/Herbaceous. Agriculture was derived from USDA Common Land Unit (CLU) data. Woody and emergent wetlands were copied from existing National Wetlands Inventory (NWI) data. Analysis of a random sampling of 612 photo-interpreted land cover reference points yielded an overall users accuracy of 86.9%. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as do

A role for Tn6029 in the evolution of the complex antibiotic resistance gene loci in genomic island 3 in enteroaggregative hemorrhagic Escherichia coli O104:H4.

PubMed

Roy Chowdhury, Piklu; Charles, Ian G; Djordjevic, Steven P

2015-01-01

In enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104 the complex antibiotic resistance gene loci (CRL) found in the region of divergence 1 (RD1) within E. coli genomic island 3 (GI3) contains blaTEM-1, strAB, sul2, tet(A)A, and dfrA7 genes encoding resistance to ampicillin, streptomycin, sulfamethoxazole, tetracycline and trimethoprim respectively. The precise arrangement of antibiotic resistance genes and the role of mobile elements that drove the evolutionary events and created the CRL have not been investigated. We used a combination of bioinformatics and iterative BLASTn searches to determine the micro-evolutionary events that likely led to the formation of the CRL in GI3 using the closed genome sequences of EAHEC O104:H4 strains 2011C-3493 and 2009EL-2050 and high quality draft genomes of EAHEC E. coli O104:H4 isolates from sporadic cases not associated with the initial outbreak. Our analyses indicate that the CRL in GI3 evolved from a progenitor structure that contained an In2-derived class 1 integron in a Tn21/Tn1721 hybrid backbone. Within the hybrid backbone, a Tn6029-family transposon, identified here as Tn6029C abuts the sul1 gene in the 3'-Conserved Segment (-CS) of a class 1 integron generating a unique molecular signature that has only previously been observed in pASL01a, a small plasmid found in commensal E. coli in West Africa. From this common progenitor, independent IS26-mediated events created two novel transposons identified here as Tn6029D and Tn6222 in 2011C-3493 and 2009EL-2050 respectively. Analysis of RD1 within GI3 reveals IS26 has played a crucial role in the assembly of regions within the CRL.

Summary appraisal of water resources in the Redmond Quadrangle, Sanpete and Sevier counties, Utah

USGS Publications Warehouse

Price, Don

1981-01-01

This map was compiled in conjunction with an energy-related geologic-mapping project on the Redmond Quadrangle (Witkind, 1980) in order to show the general availability and chemical quality of water in the area. The map is based chiefly on data collected by the U.S. Geological Survey under a continuing cooperative program with the Utah Department of Natural Resources, Division of Water Rights, and on cursory field observations by the writer. Most of the existing fata are in reports of Carpenter and Young (1963), Hahl and Cabell (1965), Young and Carpenter (1965) and Hahl and Mundorff (1968). Additional information about water and related land resources in the map area may be found in a report of the U.S. Department of Agriculture (1969).The map is intended for general planning purposes only and needs to be used with discretion. Detailed site-specific information about the availability and quality of water or about water-related problems can be gained only by special on-site investigations.

38. Photograph of a line drawing. 'PART III, SECTION 1, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

38. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS G-1 TO G-10 INCL., PURIFICATION, MANUFACTURING AREA, PLAN 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

43. Photograph of a line drawing. 'PART III, SECTION 1, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

43. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS H-1 TO H-10 INCL., GRINDING, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

77 FR 61653 - Notice of Intent To Rule on Request To Release Airport Property at the Smyrna-Rutherford County...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-10

..., Tennessee 37167 and the FAA Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN..., Manager, Memphis Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN 38118. In... Administration, Memphis Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN 38118. The...

ENTRANCE GATE AND MEMORIAL AVENUE APPROACH, LOOKING INTO CEMETERY WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

ENTRANCE GATE AND MEMORIAL AVENUE APPROACH, LOOKING INTO CEMETERY WITH ADMINISTRATION BUILDING IN BACKGROUND. VIEW TO NORTHWEST. - Mountain Home National Cemetery, Mountain Home, Washington County, TN

Intramolecular transposition by a synthetic IS50 (Tn5) derivative

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tomcsanyi, T.; Phadnis, S.H.; Berg, D.E.

1990-11-01

We report the formation of deletions and inversions by intramolecular transposition of Tn5-derived mobile elements. The synthetic transposons used contained the IS50 O and I end segments and the transposase gene, a contraselectable gene encoding sucrose sensitivity (sacB), antibiotic resistance genes, and a plasmid replication origin. Both deletions and inversions were associated with loss of a 300-bp segment that is designated the vector because it is outside of the transposon. Deletions were severalfold more frequent than inversions, perhaps reflecting constraints on DNA twisting or abortive transposition. Restriction and DNA sequence analyses showed that both types of rearrangements extended from onemore » transposon end to many different sites in target DNA. In the case of inversions, transposition generated 9-bp direct repeats of target sequences.« less

A simple and novel method for RNA-seq library preparation of single cell cDNA analysis by hyperactive Tn5 transposase.

PubMed

Brouilette, Scott; Kuersten, Scott; Mein, Charles; Bozek, Monika; Terry, Anna; Dias, Kerith-Rae; Bhaw-Rosun, Leena; Shintani, Yasunori; Coppen, Steven; Ikebe, Chiho; Sawhney, Vinit; Campbell, Niall; Kaneko, Masahiro; Tano, Nobuko; Ishida, Hidekazu; Suzuki, Ken; Yashiro, Kenta

2012-10-01

Deep sequencing of single cell-derived cDNAs offers novel insights into oncogenesis and embryogenesis. However, traditional library preparation for RNA-seq analysis requires multiple steps with consequent sample loss and stochastic variation at each step significantly affecting output. Thus, a simpler and better protocol is desirable. The recently developed hyperactive Tn5-mediated library preparation, which brings high quality libraries, is likely one of the solutions. Here, we tested the applicability of hyperactive Tn5-mediated library preparation to deep sequencing of single cell cDNA, optimized the protocol, and compared it with the conventional method based on sonication. This new technique does not require any expensive or special equipment, which secures wider availability. A library was constructed from only 100 ng of cDNA, which enables the saving of precious specimens. Only a few steps of robust enzymatic reaction resulted in saved time, enabling more specimens to be prepared at once, and with a more reproducible size distribution among the different specimens. The obtained RNA-seq results were comparable to the conventional method. Thus, this Tn5-mediated preparation is applicable for anyone who aims to carry out deep sequencing for single cell cDNAs. Copyright © 2012 Wiley Periodicals, Inc.

75 FR 25308 - Environmental Impact Statement: Winnebago County, IL and Rock County, WI

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-07

... DEPARTMENT OF TRANSPORTATION Federal Highway Administration Environmental Impact Statement: Winnebago County, IL and Rock County, WI AGENCY: Federal Highway Administration (FHWA), DOT. ACTION: Notice... Nye School Road northwest of Beloit, Rock County, Wisconsin to the interchange of Rockton Road and I...

County Government in Georgia [And] Teacher's Manual for County Government in Georgia.

ERIC Educational Resources Information Center

Hepburn, Mary A.

The student textbook and the teacher's manual focus on the services, organization, and funding of county government in Georgia. Designed to be used over a three to six week period, the textbook is arranged into six chapters. Chapter one discusses county government, its services, and its structure. Chapter two focuses on county officials and their…

2. GENERAL VIEW FROM SOUTH SHOWING SOUTHWEST AND SOUTHEAST SIDES ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. GENERAL VIEW FROM SOUTH SHOWING SOUTHWEST AND SOUTHEAST SIDES AND CLERESTORY ARRANGEMENT - Sulphur Springs Methodist Campground, Sulphur Springs Road (Sulphur Springs), Sulphur Springs, Washington County, TN

NASA Provides Coast-to-Coast Coverage of Aug. 21 Solar Eclipse (Clarksville, TN – Austin Peay)

NASA Image and Video Library

2017-08-21

On Monday, Aug. 21, NASA provided coast-to-coast coverage of the solar eclipse across America – featuring views of the phenomenon from unique vantage points, including from the ground, from aircraft, and from spacecraft including the ISS, during a live broadcast seen on NASA Television and the agency’s website. This is footage from Austin Peay State University, in Clarksville, TN.

Identifying environmental features for land management decisions. [Utah

NASA Technical Reports Server (NTRS)

1981-01-01

Advances in digital processing of LANDSAT imagery and in the interpretation of aerial photography are reported as well as the development of a geographic information system and the acquisition of cartographic equipment. Services to technical specialists and dignitaries are also described. The status of the following studies is discussed: Farmington Bay waterfowl; Sevier River wetland and agriculture; Davis County foothill development; Bear River Range aspen habitat; Wasatch-Cache riparian habitats; irrigated acreage in the Bear River Basin; the Parker Mountain rangeland inventory; and the development of techniques for inventorying aspen and aspen conifer stands. The cooperative project with NASA-Ames to verify LANDSAT digital maps of the forest and range resources on the LaSal Mountains is also considered.

44. Photograph of a line drawing. 'PLAN LAYOUT OF PART ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

44. Photograph of a line drawing. 'PLAN LAYOUT OF PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS H-1 TO H-10 INCL., GRINDING, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant 8, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

31. Photograph of a line drawing. 'PLAN LAYOUT OF PART ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. Photograph of a line drawing. 'PLAN LAYOUT OF PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS D-1 TO U-10 INCL., NITRATION, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

30. Photograph of a line drawing. 'CROSS SECTION OF PART ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

30. Photograph of a line drawing. 'CROSS SECTION OF PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS D-1 TO D-10 INCL., NITRATION, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

76 FR 52733 - Notice of Intent To Rule on Change in Use of Aeronautical Property at Bowling Green-Warren County...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-23

... the FAA Memphis Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN 38118..., Memphis Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN 38118. In addition, a... Administration, Memphis Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN 38118. The...

High-Level Heat Resistance of Spores of Bacillus amyloliquefaciens and Bacillus licheniformis Results from the Presence of a spoVA Operon in a Tn1546 Transposon

PubMed Central

Berendsen, Erwin M.; Koning, Rosella A.; Boekhorst, Jos; de Jong, Anne; Kuipers, Oscar P.; Wells-Bennik, Marjon H. J.

2016-01-01

Bacterial endospore formers can produce spores that are resistant to many food processing conditions, including heat. Some spores may survive heating processes aimed at production of commercially sterile foods. Recently, it was shown that a spoVA operon, designated spoVA2mob, present on a Tn1546 transposon in Bacillus subtilis, leads to profoundly increased wet heat resistance of B. subtilis spores. Such Tn1546 transposon elements including the spoVA2mob operon were also found in several strains of Bacillus amyloliquefaciens and Bacillus licheniformis, and these strains were shown to produce spores with significantly higher resistances to wet heat than their counterparts lacking this transposon. In this study, the locations and compositions of Tn1546 transposons encompassing the spoVA2mob operons in B. amyloliquefaciens and B. licheniformis were analyzed. Introduction of these spoVA2mob operons into B. subtilis 168 (producing spores that are not highly heat resistant) rendered mutant 168 strains that produced high-level heat resistant spores, demonstrating that these elements in B. amyloliquefaciens and B. licheniformis are responsible for high level heat resistance of spores. Assessment of growth of the nine strains of each species between 5.2°C and 57.7°C showed some differences between strains, especially at lower temperatures, but all strains were able to grow at 57.7°C. Strains of B. amyloliquefaciens and B. licheniformis that contain the Tn1546 elements (and produce high-level heat resistant spores) grew at temperatures similar to those of their Tn1546-negative counterparts that produce low-level heat resistant spores. The findings presented in this study allow for detection of B. amyloliquefaciens and B. licheniformis strains that produce highly heat resistant spores in the food chain. PMID:27994575

A Phase I Study of Unimolecular Pentavalent (Globo-H-GM2-sTn-TF-Tn) Immunization of Patients with Epithelial Ovarian, Fallopian Tube, or Peritoneal Cancer in First Remission.

PubMed

O'Cearbhaill, Roisin E; Ragupathi, Govind; Zhu, Jianglong; Wan, Qian; Mironov, Svetlana; Yang, Guangbin; Spassova, Maria K; Iasonos, Alexia; Kravetz, Sara; Ouerfelli, Ouathek; Spriggs, David R; Danishefsky, Samuel J; Sabbatini, Paul J

2016-04-22

We conducted a phase I study in ovarian cancer patients to evaluate the safety and immunogenicity of a synthetic unimolecular pentavalent carbohydrate vaccine (Globo-H, GM2, sTn, TF, and Tn) supported on a peptide backbone, conjugated to keyhole limpet haemocyanin (KLH), and mixed with immunological adjuvant QS-21. Twenty-four advanced-stage, poor-risk, first-remission ovarian cancer patients were enrolled from January 2011-Septermber 2013. Three dose levels were planned (25, 50, 100 mcg) with three cohorts of six patients each, with an additional 6-patient expansion cohort at the MTD. ELISA serologic IgM and IgG responses for each antigen was defined as positive response if antibody titers were ≥1:80 over the respective patient's pre-vaccination serum. The study would be considered positive if at least four of 12 patients treated at the MTD showed immune responses for at least three of the five antigens. Twenty-four patients (median age, 54 years [range, 36-68]) were included in the safety analysis. Histology was high-grade serous in 22 patients (92%); 18 had stage III and six stage IV disease. The vaccine was well-tolerated at all doses, with no DLTs. At the highest treated dose, IgG and/or IgM responses were recorded against ≥3 antigens in 9/12 patients (75%), ≥4 in 7/12 (58%), and 5 in 3/12 (25%). With a median follow-up of 19 months (range, 2-39), 20 patients (83%) recurred and six (25%) died. The unimolecular pentavalent vaccine construct was shown to be safe and immunogenic. Such a construct greatly simplifies regulatory requirements and manufacturing, facilitates scalability, and provides adaptability.

Use of the multipurpose transposon Tn KPK2 for the mutational analysis of chromosomal regions upstream and downstream of the sipF gene in Bradyrhizobium japonicum.

PubMed

Müller, P

2004-04-01

The DNA regions upstream and downstream of the Bradyrhizobium japonicum gene sipF were cloned by in vivo techniques and subsequently sequenced. In order to study the function of the predicted genes, a new transposon for in vitro mutagenesis, Tn KPK2, was constructed. This mutagenesis system has a number of advantages over other transposons. Tn KPK2 itself has no transposase gene, making transposition events stable. Extremely short inverted repeats minimize the length of the transposable element and facilitate the determination of the nucleotide sequence of the flanking regions. Since the transposable element carries a promoterless ' phoA reporter gene, the appearance of functional PhoA fusion proteins indicates that Tn KPK2 has inserted in a gene encoding a periplasmic or secreted protein. Although such events are extremely rare, because the transposon has to insert in-frame, in the correct orientation, and at an appropriate location in the target molecule, a direct screening procedure on agar indicator plates permits the identification of candidate clones from large numbers of colonies. In this study, Tn KPK2 was used for the construction of various symbiotic mutants of B. japonicum. One of the mutant strains, A2-10, which is defective in a gene encoding a protein that comigrates with bacterioferritin ( bcpB), was found to induce the formation of small and ineffective nodules.

Clark county monitoring program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Conway, Sheila; Auger, Jeremy; Navies, Irene

2007-07-01

Available in abstract form only. Full text of publication follows: Since 1988, Clark County has been one of the counties designated by the United States Department of Energy (DOE) as an 'Affected Unit of Local Government' (AULG). The AULG designation is an acknowledgement by the federal government that could be negatively impacted to a considerable degree by activities associated with the Yucca Mountain High Level Nuclear Waste Repository. These negative effects would have an impact on residents as individuals and the community as a whole. As an AULG, Clark County is authorized to identify 'any potential economic, social, public healthmore » and safety, and environmental impacts' of the potential repository (42 USC Section 10135(C)(1)(B)(1)). Toward this end, Clark County has conducted numerous studies of potential impacts, many of which are summarized in the Clark County's Impact Assessment Report that was submitted by the DOE and the president of the United States in February 2002. Given the unprecedented magnitude and duration of the DoE's proposal, as well as the many unanswered questions about the number of shipments and the modal mix, the estimate of impacts described in these studies are preliminary. In order to refine these estimates, Clark County Comprehensive Planning Department's Nuclear Waste Division is continuing to assess potential impacts. In addition, the County has implemented a Monitoring Program designed to capture changes to the social, environmental, and economic well-being of its residents resulting from the Yucca Mountain project and other significant events within the County. The Monitoring Program acts as an 'early warning system' that allows Clark County decision makers to proactive respond to impacts from the Yucca Mountain Project. (authors)« less

1. View of Asylum (Western) Avenue viaduct in setting from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. View of Asylum (Western) Avenue viaduct in setting from northwest, facing southeast. - Asylum Avenue Viaduct, Spanning Second Creek & Southern Railroad at State Route 62, Knoxville, Knox County, TN

CIRCULAR DRIVE, WITH ENTRANCE GATE AT LEFT FOREGROUND AND UNION ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

CIRCULAR DRIVE, WITH ENTRANCE GATE AT LEFT FOREGROUND AND UNION SOLDIERS’ MONUMENT AT CENTER BACKGROUND. VIEW TO SOUTH. - Knoxville National Cemetery, 939 Tyson Street, Northwest, Knoxville, Knox County, TN

3. Threequarter elevation view of Asylum (Western) viaduct from southwest, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. Three-quarter elevation view of Asylum (Western) viaduct from southwest, facing northeast. - Asylum Avenue Viaduct, Spanning Second Creek & Southern Railroad at State Route 62, Knoxville, Knox County, TN

7. GENERAL VIEW OF INTERIOR OF MEETINGHOUSE FROM SOUTHEAST SHOWING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. GENERAL VIEW OF INTERIOR OF MEETINGHOUSE FROM SOUTHEAST SHOWING CLERESTORY ARRANGEMENT AND SUPPORT SYSTEM - Sulphur Springs Methodist Campground, Sulphur Springs Road (Sulphur Springs), Sulphur Springs, Washington County, TN

County portraits of Washington State.

Treesearch

Wendy J. McGinnis; Richard H. Phillips; Terry L. Raettig; Kent P. Connaughton

1997-01-01

This publication provides a general picture of the population, economy, and natural resources of the counties in Washington State. The intent of this report is to provide insight to changes in a county over the last 10 to 20 years, to compare county trends to statewide trends (and state trends to national trends), and to provide information on all the counties in a...

76 FR 30152 - East Calloway County Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-24

... Middle School Mercury Spill Site, Murray, Calloway County, KY; Notice of Settlement AGENCY: Environmental... Calloway County Middle School Mercury Spill Site located in Murray, Calloway County, Kentucky for... County [[Page 30153

HISTORIC IMAGE: AERIAL VIEW OF THE CEMETERY AND ITS ENVIRONS. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

HISTORIC IMAGE: AERIAL VIEW OF THE CEMETERY AND ITS ENVIRONS. PHOTOGRAPH TAKEN ON 18 MAY 1948. NCA HISTORY COLLECTION. - Knoxville National Cemetery, 939 Tyson Street, Northwest, Knoxville, Knox County, TN

2. Threequarter elevation view of Asylum (Western) Avenue viaduct from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Three-quarter elevation view of Asylum (Western) Avenue viaduct from northwest, facing southeast. - Asylum Avenue Viaduct, Spanning Second Creek & Southern Railroad at State Route 62, Knoxville, Knox County, TN

The Economic Impact of Schenectady County Community College on Schenectady County, 1981-82.

ERIC Educational Resources Information Center

Chestnut, Erma Ruth

This report on the economic impact of Schenectady County Community College (SCCC) uses a modification of the Caffrey and Isaacs model to assess SCCC-related local business volume, SCCC costs and benefits to the Schenectady County government, and the likely impact on the county if SCCC did not exist. Part I provides background to the study,…

Morris County Improvement Authority, Morris County, New Jersey Renewable Energy Initiative

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bonanni, John

2013-05-01

The Morris County Improvement Authority (Authority), a public body corporate and politic of the State of New Jersey and created and controlled by the County, at the direction of the County and through the Program guaranteed by the County, financed 3.2 MW of solar projects (Solar Projects) at fifteen (15) sites for seven (7) local government units (Local Units) in and including the County. The Program uses a Power Purchase Agreement (PPA) structure, where the Solar Developer constructs, operates and maintains all of the Solar Projects, for the benefit of the Local Units and the Authority, for the maximum Statemore » law allowable PPA period of fifteen (15) years. Although all fifteen (15) sites were funded by the Authority, only the Mennen Arena site was considered for the purposes of the required local match funding for this grant. Specifically at the Mennen Arena site, the Authority financed 1.6 MW of solar panels. On October 18, 2013, the DOE Grant was drawn down following completion of the necessary application documents and final execution of an agreement memorializing the contemplated transaction by the Local Units, the County, The Authority and the solar developer. The proceeds of the DOE Grant were then applied to reduce the PPA price to all Local Units across the program and increase the savings from approximately 1/3 to almost half off the existing and forecasted utility pricing over the fifteen (15) year term, without adversely affecting all of the other benefits. With the application of the rate buy down, the price of electricity purchased under the PPA dropped from 10.9 to 7.7 cents/kWh. This made acquisition of renewable energy much more affordable for the Local Units, and it enhanced the success of the program, which will encourage other counties and local units to develop similar programs.« less

[Establishment of Reference Value of Hs-cTnT in Sichuan Region and Its Diagnostic Value in Patients with Chest Pain].

PubMed

Guo, Ying; Du, Xiang-Yang; Huang, Hua-Lan; Wang, Wei-Qing; Nie, Xin; Li, Gui-Xing

2017-11-01

To establish the reference value of high sensitive cardiac troponin T (hs-cTnT) and the efficiency of reference value in the diagnosis of chest pain. Volunteers from eight independent communities in Chengdu,Sichuan were selected with detailed records of physical examination,electrocardiogram,ultrasound examination. The level of hs-cTnT for healthy volunteers was tested to determine ninety-ninth percentile references according to sex and ages. 2 249 patients with chest pain in the emergency department of Western China Hospital from July 2009 to July 2014 were enrolled to measure the efficiency of reference value for diagnosing acute myocardial infarction (AMI). There were 1 305 volunteers included finally. Among them,the mean hs-cTnT level of male was 4.3 (3.2-5.9) ng/L,which was significantly higher than that of female 3.0 (3.0-3.1) ng/L ( P <0.01) . The correlation coefficient between age and hs-cTnT level was 0.43 (male) and 0.29 (female),and the P -value was less than 0.01. The 99th percentile values of male were 10.8 ng/L,15.4 ng/L and 19.7 ng/L for <45 yr.,45-<60 yr. and ≥60 yr.,respectively. Those values of female were 4.6 ng/L,8.9 ng/L,18.8 ng/L,respectively. There was no difference in sensitivity and specificity between the value we figured out and manufactures provided (14.0 ng/L) for those<60 yr.. For the patients ≥60 yr.,the sensitivity and negative predictive value did not show diversity ( P >0.05) but the specificity and positive predictive value showed significant difference (male: 0.67 vs. 0.56 and 0.83 vs. 0.79, P <0.05;female:0.75 vs. 0.68 and 0.74 vs. 0.69, P <0.05). We recommends that the ninety-ninth percentile reference value of patients<60 yr. should be 14.0 ng/L,while 20.0 ng/L for those patients≥60 yr.

37. Photograph of a line drawing. 'PART I, SECTION 8, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

37. Photograph of a line drawing. 'PART I, SECTION 8, BUILDINGS NO. G-1 TO G-10 INCL., PURIFICATION, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

52. Photograph of a line drawing. 'PART I, SECTION 8, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

52. Photograph of a line drawing. 'PART I, SECTION 8, BUILDINGS NO. 0-1, 0-3, 0-5, 0-7, 0-9, TESTING LABORATORY, MANUFACTURING AREA, PLANT B.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

10. CUT OF THE CHURCH BUILDING AS IT APPEARED ON ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. CUT OF THE CHURCH BUILDING AS IT APPEARED ON THE PRINTED PROGRAM OF THE JANUARY 1, 1893 DEDICATION SERVICE. - Second Presbyterian Church, Pontatoc Avenue & Hernando Street, Memphis, Shelby County, TN

Green digital signage using nanoparticle embedded narrow-gap field sequential TN-LCDs

NASA Astrophysics Data System (ADS)

Kobayashi, Shunsuke; Shiraishi, Yukihide; Sawai, Hiroya; Toshima, Naoki; Okita, Masaya; Takeuchi, Kiyofumi; Takatsu, Haruyoshi

2012-03-01

We have fabricated field sequential color (FSC)-LCDs using cells and modules of narrow-gap TN-LCDs with and without doping the nanoparticles of PCyD-ZrO2 and AF-SiO2. It is shown that the FSC-LCD exhibits a high optical efficiency of OE=4.5 that is defined as OE=[Luminance]/[W/m2]=(cd/W). This figure may provide us a good reference or to clear the Energy Star Program Version 5-3 that issues a guideline: LCD with 50 inch on the diagonal consumes the energy of 108W. Through this research it is claimed that our FSC=LCD may be a novel green digital signage.

'Test n Treat (TnT)': a cluster-randomised feasibility trial of frequent, rapid-testing and same-day, on-site treatment to reduce rates of chlamydia in high-risk further education college students: statistical analysis plan.

PubMed

Phillips, Rachel; Oakeshott, Pippa; Kerry-Barnard, Sarah; Reid, Fiona

2018-06-05

There are high rates of sexually transmitted infections (STIs) in ethnically diverse, sexually active students aged 16-24 years attending London further education (FE) colleges. However, uptake of chlamydia screening remains low. The TnT study aims to assess the feasibility of conducting a future trial in FE colleges to investigate if frequent, rapid, on-site testing and treatment (TnT) reduces chlamydia rates. This article presents the statistical analysis plan for the main study publication as approved and signed off by the Trial Management Group prior to the first data extraction for the final report. TnT is a cluster-randomised feasibility trial conducted over 7 months with parallel qualitative and economic assessments. Colleges will be randomly allocated into the intervention (TnT) or the control group (no TnT). Six FE colleges in London will be included. At each college for 2 days, 80 consecutive sexually active students aged 16-24 years (total 480 students across all six colleges) will be recruited from public areas and asked to provide baseline samples. One and 4 months after recruitment intervention colleges will be visited on two consecutive days by the TnT team where participating students will be texted and invited to come for same-day, on-site, rapid chlamydia testing and, if positive, treatment. Participants in the control colleges will receive 'thank you' texts 1 and 4 months after recruitment. Seven months after recruitment, participants from both groups will be invited to complete questionnaires and provide samples for TnT. All samples will be tested, and same-day treatment offered to participants with positive results. Key feasibility outcomes include: recruitment rates, testing and treatment uptake rates (at 1 and 4 months) and follow-up rates (at 7 months). ISRCTN 58038795 . Registered on 31 August 2016.

CROSS DRIVE BETWEEN SECTION A (RIGHT) AND SECTION B (LEFT), ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

CROSS DRIVE BETWEEN SECTION A (RIGHT) AND SECTION B (LEFT), WITH FLAGPOLE AND COMMITTAL SHELTER AT CENTER BACKGROUND. VIEW TO NORTHWEST. - Knoxville National Cemetery, 939 Tyson Street, Northwest, Knoxville, Knox County, TN

13. Building H9; view of stainless steel probes and vacuum ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Building H-9; view of stainless steel probes and vacuum line, looking W. (Ryan and Harms) - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

Chapter 15 - Thousand Cankers Disease in Tennessee: For how long? (Project SO-EM-B-11-01)

Treesearch

KaDonna Randolph

2014-01-01

In August 2010, the Tennessee Department of Agriculture (TDA) announced the discovery of the walnut twig beetle (WTB) (Pityophthorus juglandis) and associated fungus Geosmithia morbida in Knox County (TN.gov Newsroom 2010).

Hydrology of Polk County, Florida

USGS Publications Warehouse

Spechler, Rick M.; Kroening, Sharon E.

2007-01-01

Local water managers usually rely on information produced at the State and regional scale to make water-resource management decisions. Current assessments of hydrologic and water-quality conditions in Polk County, Florida, commonly end at the boundaries of two water management districts (South Florida Water Management District and the Southwest Florida Water Management District), which makes it difficult for managers to determine conditions throughout the county. The last comprehensive water-resources assessment of Polk County was published almost 40 years ago. To address the need for current countywide information, the U.S. Geological Survey began a 3?-year study in 2002 to update information about hydrologic and water-quality conditions in Polk County and identify changes that have occurred. Ground-water use in Polk County has decreased substantially since 1965. In 1965, total ground-water withdrawals in the county were about 350 million gallons per day. In 2002, withdrawals totaled about 285 million gallons per day, of which nearly 95 percent was from the Floridan aquifer system. Water-conservation practices mainly related to the phosphate-mining industry as well as the decrease in the number of mines in operation in Polk County have reduced total water use by about 65 million gallons per day since 1965. Polk County is underlain by three principal hydrogeologic units. The uppermost water-bearing unit is the surficial aquifer system, which is unconfined and composed primarily of clastic deposits. The surficial aquifer system is underlain by the intermediate confining unit, which grades into the intermediate aquifer system and consists of up to two water-bearing zones composed of interbedded clastic and carbonate rocks. The lowermost hydrogeologic unit is the Floridan aquifer system. The Floridan aquifer system, a thick sequence of permeable limestone and dolostone, consists of the Upper Floridan aquifer, a middle semiconfining unit, a middle confining unit, and

Somerset County Renewable Energy Initiative

DOE Office of Scientific and Technical Information (OSTI.GOV)

Katula, Denise

2014-05-07

The County of Somerset, New Jersey, through the Somerset County Improvement Authority (SCIA), applied Federal funding through the U.S. Department of Energy to will apply project funds to buy-down the capital costs of equipment associated with the installation of solar photovoltaic (PV) systems at two sites owned by the County. This Renewable Energy Initiative allows the County to take advantage of clean renewable energy, without any adverse debt impacts, and at a price that results in operating budget savings beyond what is presently available in the marketplace. This project addressed the objectives of the Office of Energy Efficiency and Renewablemore » Energy by making the acquisition of renewable energy more affordable for the County, thereby, encouraging other counties and local units to develop similar programs and increase the deployment of solar energy technologies. The two sites that were funded by the DOE grant are part of a much larger, ambitious, and unique renewable energy project, described in the next section.« less

6. Through/barrel view of Asylum (Western) Avenue bridge from west ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Through/barrel view of Asylum (Western) Avenue bridge from west end looking east/downtown, facing southeast. - Asylum Avenue Viaduct, Spanning Second Creek & Southern Railroad at State Route 62, Knoxville, Knox County, TN

5. Through/barrel view of Asylum (Western) Avenue viaduct from downtown/east ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Through/barrel view of Asylum (Western) Avenue viaduct from downtown/east end looking west, facing northwest. - Asylum Avenue Viaduct, Spanning Second Creek & Southern Railroad at State Route 62, Knoxville, Knox County, TN

9. Overall underneath view of Asylum (Western) Avenue viaduct, from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Overall underneath view of Asylum (Western) Avenue viaduct, from east end/downtown toward west, facing northwest. - Asylum Avenue Viaduct, Spanning Second Creek & Southern Railroad at State Route 62, Knoxville, Knox County, TN

4. Historic American Buildings Survey, W. Jeter EasonDeputy District Officer, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Historic American Buildings Survey, W. Jeter Eason-Deputy District Officer, Photographer August 3, 1936 OPEN 'DOG-TROT' PORCH (LOOKING SOUTH). - Wynnewood, Gallatin-Hartsville Pike (State Highway 25), Castalian Springs, Sumner County, TN

Successful practices in title III implementation. Chemical Emergency Preparedness and Prevention Technical Assistance Bulletin. Cameron County, Texas; Bucks County, Pennsylvania; Harford County, Maryland; Dallas County, Texas. Series 6, Number 7

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This is another in a series of bulletins EPA is issuing to provide examples of implementation programs and strategies of the Emergency Planning and Community Right-to-Know Act of 1986, known as Title III, that are innovative or have proven effective. The purpose of these bulletins is to share information on successful practices with Local Emergency Planning Committees (LEPCs), State Emergency Response Commissions (SERCs), fire departments, and other Title III implementing agencies throughout the country in the hope that such information will prove useful to other SERCs and LEPCs as their programs develop and evolve. The bulletin discusses Title III implementationmore » for Cameron County in Texas, Bucks County in Pennsylvania, Harford County in Maryland, and Dallas County in Texas.« less

42. Photograph of a line drawing. 'CROSS SECTION AND PLAN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

42. Photograph of a line drawing. 'CROSS SECTION AND PLAN LAYOUT OF PART I, SECTION 8, BUILDINGS NO. H-1 TO H-10 INCL., GRINDING, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (NashVille, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

29. Photograph of a line drawing. 'CROSS SECTION AND PLAN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. Photograph of a line drawing. 'CROSS SECTION AND PLAN LAYOUT OF PART I, SECTION 8, BUILDINGS NO. D-1 TO D-10 INCL., NITRATION, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

Fuelwood use in U.S. counties

Treesearch

Kenneth E. Skog; Robert S. Manthy

1989-01-01

This study explains and determines fuelwood consumption at the county level based on county economic and demographic conditions, and identifies U.S. counties where potential fuelwood use problems and benefits are greatest. The percentage of wood-burning households in a county is estimated and multiplied by estimated average wood consumed per wood-burning household in...

77 FR 14032 - John H. Chafee Coastal Barrier Resources System; Lee County, FL, and Newport County, RI...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-08

..., FXHC11240900000T5, 123] John H. Chafee Coastal Barrier Resources System; Lee County, FL, and Newport County, RI... Lee County, Florida. The second map, dated September 30, 2009, is for four CBRS units located in... by Lee County, and 1 restaurant. The Service's assessment of 2011 aerial imagery estimates that the...

EnviroAtlas -- Memphis, TN (2012) -- One Meter Resolution Urban Land Cover Data Web Service

EPA Pesticide Factsheets

This EnviroAtlas web service supports research and online mapping activities related to EnviroAtlas (https://www.epa.gov/enviroatlas ). The Memphis, TN EnviroAtlas One Meter-scale Urban Land Cover (MULC) dataset comprises 2,733 km2 around the city of Memphis, surrounding towns, and rural areas. These leaf-on LC data and maps were derived from 1-m pixel, four-band (red, green, blue, and near-infrared) aerial photography acquired from the United States Department of Agriculture (USDA) National Agriculture Imagery Program (NAIP) on four dates in 2012: June 15, June 18, June 21 and June 23, and one date in 2013: July 12. Three separate LiDAR (Light Detection and Ranging) data sets collected on February 19, 2009 00e2?? August 2, 2010, December 1-2, 2011 and January 23-24, 2012 were integrated for Shelby Co., TN, Crittenden Co., AR, and DeSoto Co, MS. Five MULC classes were mapped directly from the NAIP and LiDAR data: Water, Impervious, Soil, Trees, and Grass/Herbaceous. Agriculture was derived from USDA Common Land Unit (CLU) data. Woody and emergent wetlands were copied from existing National Wetlands Inventory (NWI) data. Analysis of a random sampling of 612 photo-interpreted land cover reference points yielded an overall users accuracy of 86.9%. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-u

How Are Kentucky's Children Stacking Up? A County by County Analysis.

ERIC Educational Resources Information Center

Chandler, Betsy

In a county by county analysis, this report assesses the quality of life for Kentucky's children. Researchers developed a child quotient (CQ) based on 18 indicators: per capita income, children in poverty, women receiving inadequate prenatal care, infant deaths, teens giving birth, substandard dwellings, children in foster care, per-pupil…

Mansonia titillans: New Resident Species or Infrequent Visitor in Chatham County, Georgia, and Beaufort County, South Carolina, USA.

PubMed

Moulis, Robert A; Peaty, Laura F A W; Heusel, Jeffrey L; Lewandowski, Henry B; Harrison, Bruce A; Kelly, Rosmarie; Hager, Elizabeth J

2015-06-01

In September, October, and November 2014, adult Mansonia titillans were collected at 4 separate sites near Savannah in Chatham County, Georgia, and 1 site in Muscogee County, GA, during routine mosquito surveillance. Although previously recorded from Beaufort County, SC, and several inland southern Georgia counties, recent reports of this species from coastal Georgia or South Carolina are lacking. These newly captured Ma. titillans specimens represent the first documented records for Muscogee County and Chatham County, GA, and may indicate a recent northern expansion or reintroduction of this species along the Georgia and South Carolina coast.

4. Threequarter view of Asylum (Western) Avenue viaduct and ramp ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Three-quarter view of Asylum (Western) Avenue viaduct and ramp on southeast corner of bridge, facing northwest. - Asylum Avenue Viaduct, Spanning Second Creek & Southern Railroad at State Route 62, Knoxville, Knox County, TN

8. Building G9; view of No. 8 condenser and top ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. Building G-9; view of No. 8 condenser and top of dissolve tank; third floor, looking S. (Ryan) - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

21. Historic American Buildings Survey, W. Jeter Eason Deputy District ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

21. Historic American Buildings Survey, W. Jeter Eason- Deputy District Officer, Photographer November 21, 1936 DETAIL OF DECORATIVE STAIR RISERS. - The Hermitage, U.S. Highway 70 North (4580 Rachel's Lane), Nashville, Davidson County, TN

33. HISTORIC PLAQUE MARKING WHERE JOHNSTON DIED, ADJACENT TO PATHWAY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. HISTORIC PLAQUE MARKING WHERE JOHNSTON DIED, ADJACENT TO PATHWAY WITH CONCRETE CULVERT LEADING NORTH OUT OF RAVINE TOWARD JOHNSTON MEMORIAL SITE. VIEW NW. - Shiloh National Military Park Tour Roads, Shiloh, Hardin County, TN

Characterization of Tn3000, a Transposon Responsible for blaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India.

PubMed

Campos, Juliana Coutinho; da Silva, Maria José Félix; dos Santos, Paulo Roberto Nascimento; Barros, Elaine Menezes; Pereira, Mayne de Oliveira; Seco, Bruna Mara Silva; Magagnin, Cibele Massotti; Leiroz, Leonardo Kalab; de Oliveira, Théo Gremen Mimary; de Faria-Júnior, Célio; Cerdeira, Louise Teixeira; Barth, Afonso Luís; Sampaio, Suely Carlos Ferreira; Zavascki, Alexandre Prehn; Poirel, Laurent; Sampaio, Jorge Luiz Mello

2015-12-01

In Enterobacteriaceae, the blaNDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the blaNDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFIIK and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the blaNDM-1 gene may be transmitted by Tn3000 in different parts of the world. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

75 FR 26709 - Clarke County Water Supply Project, Clarke County, IA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-12

... Project, Clarke County, IA AGENCY: Natural Resources Conservation Service. ACTION: Notice of intent to... Conservationist for Planning, 210 Walnut Street, Room 693, Des Moines, IA 50309-2180, telephone: 515-284- 4769... available at the Iowa NRCS Web site at http://www.ia.nrcs.usda.gov . A map of the Clarke County Water Supply...

The timber resources of Humboldt County, California.

Treesearch

Daniel D. Oswald

1968-01-01

This report presents the first complete inventory of Humboldt County's timber resources. Past Forest Survey inventories have included Humboldt County, but they were not designed to obtain volume estimates for an individual county. Humboldt County is part of a survey unit which also includes Del Norte County. There are eight such inventory units in California; and...

Population substructure in Cache County, Utah: the Cache County study

PubMed Central

2014-01-01

Background Population stratification is a key concern for genetic association analyses. In addition, extreme homogeneity of ethnic origins of a population can make it difficult to interpret how genetic associations in that population may translate into other populations. Here we have evaluated the genetic substructure of samples from the Cache County study relative to the HapMap Reference populations and data from the Alzheimer's Disease Neuroimaging Initiative (ADNI). Results Our findings show that the Cache County study is similar in ethnic diversity to the self-reported "Whites" in the ADNI sample and less homogenous than the HapMap CEU population. Conclusions We conclude that the Cache County study is genetically representative of the general European American population in the USA and is an appropriate population for conducting broadly applicable genetic studies. PMID:25078123

The impact of systems-of-care on pharmacoinvasive management with streptokinase: The subgroup analysis of the TN-STEMI programme.

PubMed

Raja, Deep Chandh; Subban, Vijayakumar; Victor, Suma M; Joseph, George; Thomson, Viji Samuel; Kannan, Kumaresan; Gnanaraj, Justin Paul; Veerasekar, Ganesh; Thenpally, Jose G; Livingston, Nandhini; Nallamothu, Brahmajee K; Alexander, Thomas; Mullasari, Ajit S

We evaluated the impact of implementation of the TN-STEMI programme on various characteristics of the pharmacoinvasive group by comparing clinical as well as angiographic outcomes between the pre- and post-implementation groups. The TN-STEMI programme involved 2420 patients of which 423 patients had undergone a pharmacoinvasive strategy of reperfusion. Of these, 407 patients had a comprehensive blinded core-lab evaluation of their angiograms post-lysis and clinical evaluation of various parameters including time-delays and adverse cardio- and cerebro-vascular events at 1year. Streptokinase was used as the thrombolytic agent in 94.6% of the patients. In the post-implementation phase, there was a significant improvement in 'First medical contact (FMC)-to-ECG' (11 vs. 5min, p<0.001) and 'Lysis-to-angiogram' (98.3 vs. 18.2h, p<0.001) times. There was also a significant improvement in the number of coronary angiograms performed within 24h (20.7% vs. 69.3%, p<0.001). The 'Time-to-FMC' (160 vs. 135min, p=0.07) and 'Total ischemic time' (210 vs. 176min, p=0.22) also showed a decreasing trend. IRA patency rate (70.2% vs. 86%, p<0.001) and thrombus burden (TIMI grade 0: 49.1% vs. 73.4%, p<0.001) were superior in this group. The MACCE rates were similar except for fewer readmissions (29.8% vs. 12.6%, p=0.0002) and target revascularizations at 1year (4.8% vs. none, p=0.002) in the post-implementation group. The implementation of a system-of-care (hub-and-spoke model) in the pharmacoinvasive group of the TN-STEMI programme demonstrated shorter lysis-to-angiogram times, better TIMI flow patterns and lower thrombus burden in the post-implementation phase. Copyright © 2017. Published by Elsevier B.V.

23. VIEW LOOKING UPSTREAM FROM WEST BANK OF HEADRACE SHOWING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. VIEW LOOKING UPSTREAM FROM WEST BANK OF HEAD-RACE SHOWING RECONSTRUCTED MAIN AND DIVERSION DAMS; HEAD-RACE IS JUST OUT OF PICTURE AT LEFT. - Forge Creek Dam-John Cable Mill, Townsend, Blount County, TN

Influence of Peer Pressure on Secondary School Students Drop out in Rongo Sub-County, Migori County, Kenya

ERIC Educational Resources Information Center

Omollo, Atieno Evaline; Yambo, Onyango J. M.

2017-01-01

The purpose of this study was to establish the influence of peer pressure on secondary school students' drop out in Rongo Sub-County, Migori County, Kenya. The statement of the problem showed that the sub-county had a dropout rate of 43 percent as compared to the neighboring sub counties like Uriri, Awendo, Nyatike, Kuria and Migori which had 25,…

75 FR 13297 - Southeastern Lincoln County Habitat Conservation Plan, Lincoln County, NV

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

... development), flood control activities (within the City of Caliente), maintenance of Lincoln County roads and... library locations: (1) Clark County Library, 1401 E. Flamingo Road, Las Vegas, NV 89119; (702) 507-3400...- administered land. Proposed covered activities include: (1) Planned land development and maintenance activities...

A large inversion in the linear chromosome of Streptomyces griseus caused by replicative transposition of a new Tn3 family transposon.

PubMed

Murata, M; Uchida, T; Yang, Y; Lezhava, A; Kinashi, H

2011-04-01

We have comprehensively analyzed the linear chromosomes of Streptomyces griseus mutants constructed and kept in our laboratory. During this study, macrorestriction analysis of AseI and DraI fragments of mutant 402-2 suggested a large chromosomal inversion. The junctions of chromosomal inversion were cloned and sequenced and compared with the corresponding target sequences in the parent strain 2247. Consequently, a transposon-involved mechanism was revealed. Namely, a transposon originally located at the left target site was replicatively transposed to the right target site in an inverted direction, which generated a second copy and at the same time caused a 2.5-Mb chromosomal inversion. The involved transposon named TnSGR was grouped into a new subfamily of the resolvase-encoding Tn3 family transposons based on its gene organization. At the end, terminal diversity of S. griseus chromosomes is discussed by comparing the sequences of strains 2247 and IFO13350.

Relationship between Job Satisfaction of County Extension Staff and the Level of Emotional Intelligence of County Extension Directors

ERIC Educational Resources Information Center

Villard, Judith A.; Earnest, Garee W.

2006-01-01

This descriptive-correlational study used a census of Ohio State University Extension county directors and a random sample of county staff throughout the State of Ohio. Data were collected utilizing Bar-On's Emotional Intelligence Quotient instrument (county directors) and Warner's job satisfaction instrument (county staff). The study examined the…

Water resources of Sweetwater County, Wyoming

USGS Publications Warehouse

Mason, Jon P.; Miller, Kirk A.

2004-01-01

Sweetwater County is located in the southwestern part of Wyoming and is the largest county in the State. A study to quantify the availability and describe the chemical quality of surface-water and ground-water resources in Sweetwater County was conducted by the U.S. Geological Survey in cooperation with the Wyoming State Engineers Office. Most of the county has an arid climate. For this reason a large amount of the flow in perennial streams within the county is derived from outside the county. Likewise, much of the ground-water recharge to aquifers within the county is from flows into the county, and occurs slowly. Surface-water data were not collected as part of the study. Evaluations of streamflow and stream-water quality were limited to analyses of historical data and descriptions of previous investigations. Forty-six new ground-water-quality samples were collected as part of the study and the results from an additional 782 historical ground-water-quality samples were reviewed. Available hydrogeologic characteristics for various aquifers throughout the county also are described. Flow characteristics of streams in Sweetwater County vary substantially depending on regional and local basin characteristics and anthropogenic factors. Because precipitation amounts in the county are small, most streams in the county are ephemeral, flowing only as a result of regional or local rainfall or snowmelt runoff. Flows in perennial streams in the county generally are a result of snowmelt runoff in the mountainous headwater areas to the north, west, and south of the county. Flow characteristics of most perennial streams are altered substantially by diversions and regulation. Water-quality characteristics of selected streams in and near Sweetwater County during water years 1974 through 1983 were variable. Concentrations of dissolved constituents, suspended sediment, and bacteria generally were smallest at sites on the Green River because of resistant geologic units, increased

27. Photograph of a line drawing. 'PLAN LAYOUT AND CROSS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. Photograph of a line drawing. 'PLAN LAYOUT AND CROSS SECTION OF PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS C-1, C-3, C-5, C-6, C-7, C-9 INCL., MIXING, MANUFACTURING AREA, PLANT 'B'.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

46. Photograph of a published page. 'OPERATIONS IN INCORPORATION BUILDINGS: ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

46. Photograph of a published page. 'OPERATIONS IN INCORPORATION BUILDINGS: HOLSTON DEFENSE CORPORATION. 'HOLSTON ARMY AMMUNITION PLANT.' Page 17. (no date). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

VIEW ALONG FREEDOM ROAD, WITH UPRIGHT HEADSTONES IN SECTION OO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW ALONG FREEDOM ROAD, WITH UPRIGHT HEADSTONES IN SECTION OO AT RIGHT FOREGROUND AND FLAT HEADSTONES IN SECTIONS O AND P AT LEFT BACKGROUND. VIEW TO SOUTHWEST. - Mountain Home National Cemetery, Mountain Home, Washington County, TN

Plinian vs. phreatomagmatic eruptions at Grímsvötn volcano, Iceland

NASA Astrophysics Data System (ADS)

Haddadi, Baptiste; Sigmarsson, Olgeir; Larsen, Guðrún

2016-04-01

Grímsvötn is a subglacial central volcano located under the Vatnajökull ice cap, above the assumed centre of the Iceland mantle plume. Historical explosive eruptions are mostly of phreatomagmatic character whereas pure magmatic behaviour may characterize the largest eruptions. What causes this different eruption behaviour is uncertain. Here, we report petrological estimates of crystallization depth and volatile degassing as recorded by sulfur concentrations in melt inclusions (MI) hosted by ferromagnesian minerals and the groundmass glass. Tephra from four eruptions, AD 1823, 1873, 2004 and 2011, were selected. The 2011 and 1873 are the largest known historical eruptions, whereas the 2004 eruption is probably amongst the smallest. The repose time preceding those eruptions is surprisingly similar, or 6 to 7 years, and the major-element compositions are uniform. Plagioclase, clinopyroxene (cpx) and olivine are the three coexisting phases at the liquidus in the quartz-tholeiites of Grímsvötn. The cpx-melt geothermobarometer (Putirka 2008) applied to the 2011 tephra reveals that cpx crystallized over a large range of P from 60 to 640 MPa (depth range: 1.7-18km) and T between 1060 and 1175°C before the Plinian eruption, therefore mobilizing the entire crustal magma system. In contrast, the phreatomagmatic tephra do not record the shallowest crystallization but interestingly all four tephra have identical median entrapment pressure of approximately 400 MPa. Therefore, the depth from which the magma bodies are derived, does not explain the difference in explosivity between those eruptions nor the variable magma volume (V) produced. Sulfur concentrations in MI are only slightly higher in the Plinian products, the difference (10%) being insufficient to explain the different eruption regimes. The ΔS, the difference between the maximum S concentrations in MI and the mean of the groundmass glass for a given eruption, is higher in the Plinian tephra. Based on literature

Bio-preservative effect of the essential oil of the endemic Mentha piperita used alone and in combination with BacTN635 in stored minced beef meat.

PubMed

Smaoui, Slim; Hsouna, Anis Ben; Lahmar, Aida; Ennouri, Karim; Mtibaa-Chakchouk, Ahlem; Sellem, Imen; Najah, Soumaya; Bouaziz, Mohamed; Mellouli, Lotfi

2016-07-01

The major compounds in Mentha piperita essential oil (EOMP) were menthol (33.59%) and iso-menthone (33%). The biopreservative effect of EOMP used alone at 0.25 or 0.5% and in combination with the semi-purified bacteriocin BacTN635 at 500 or 1000AU/g, on minced beef meat was evaluated by microbiological, physicochemical and sensory analyses during storage at 4°C for 21days. EOMP used alone limited the microbial deterioration of minced meat (P<0.05). Furthermore, the combination between EOMP and BacTN635 led to a decrease in TBARS values and slowed down the accumulation of MetMb. This combination was more efficient (P<0.05) against microflora proliferation and enhanced the sensory acceptability extending thus the shelf life of meat beef by approximately 7days. On the basis of these results, physicochemical and sensorial parameters could be used for constructing regression models to predict overall acceptability. Overall, the strongest preservative effect was achieved by using the combination of EOMP at 0.5% with BacTN535 at 1000AU/g. Copyright © 2016 Elsevier Ltd. All rights reserved.

All Prime Contract Awards by State or Country, Place, and Contractor, FY 88. Part 1. (Aliceville, Alabama-Sevier, Arkansas).

DTIC Science & Technology

1988-01-01

W0(D1- 4V444-t * 400O-4 1 X:00P-- (01 - r-r- Z 30 I- L 0U) uU)z 04 * Wu n ) enC ’)4 z 00002 IN-4I0o--q I (DUU 4ni 0000 0N 4 < WOOOOO 0 X:0 2000 -.4...0 o04ɘ c) I 4NN 0)N 00 - NNrN r- "C4TN)C C4 LAN (IN rN (N- 0) r4 W I DCI) ’(00 000 0000 oco lADO (00 LAO 4 0 0O 0)0 Ln CO OIDC 1 O n IM< L < 0"-<ɘ...0 - a0 C-) 11000 ’t4 LADO f-O 0 <am IINLA 3 r U-0- - ( w< <- n(00l NLA (𔃺(<N< 00N7 O <OC <-LLA 0 140" I CI) (0 LA Ln -O.40 ) Ln- L a0 0Y) D(0

Digital computer processing of LANDSAT data for North Alabama. [Linestone County, Madison County, Jackson County, Marshall County, and DeKalb County

NASA Technical Reports Server (NTRS)

Bond, A. D.; Atkinson, R. J.; Lybanon, M.; Ramapriyan, H. K.

1977-01-01

Computer processing procedures and programs applied to Multispectral Scanner data from LANDSAT are described. The output product produced is a level 1 land use map in conformance with a Universal Transverse Mercator projection. The region studied was a five-county area in north Alabama.

County Community Development Studies. No. 2: Extensions Library on Tape, Sarasota County.

ERIC Educational Resources Information Center

Rozar, Luther L.; And Others

A study was conducted in Sarasota County, Florida, to determine the feasibility of implementing a County Extension Service library on telecassette tape that could be dialed by phone callers. The system would allow office callers access to two-to-three-minute tape recordings containing information on many common problems. A survey instrument…

Albemarle County road orders, 1783-1816.

DOT National Transportation Integrated Search

1975-01-01

During the early stages of the pilot study of Albemarle County it was necessary to examine and extract all the road orders for the counties from which Albemarle was formed, as well as the orders for Albemarle when it still contained the counties of A...

Agreement between the County of Sullivan and Sullivan County Community College and the Professional Staff Association of Sullivan County Community College, September 1, 1987-August 31, 1990.

ERIC Educational Resources Information Center

Sullivan County Community Coll., Loch Sheldrake, NY.

This collective bargaining agreement between the County of Sullivan and Sullivan County Community College and the Professional Staff Association of Sullivan County Community College establishes conditions of employment for academic teaching, non-teaching, and support staff. The articles in the agreement set forth provisions related to: (1)…

45. Photograph of a published page. OPERATIONS IN 'H' OR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

45. Photograph of a published page. OPERATIONS IN 'H' OR DEWATERING BUILDING: HOLSTON DEFENSE CORPORATION. 'HOLSTON ARMY AMMUNITION PLANT.' Page 16. (no date). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

Coping with Resource Management Challenges in Mumias Sub-County, Kakamega County, Kenya

ERIC Educational Resources Information Center

Anyango, Onginjo Rose; Orodho, John Aluko

2016-01-01

The gist of the study was to examine the main coping strategies used to manage resources in public secondary schools in Mumias Sub-County, Kakamega County, Kenya. The study was premised on Hunts (2007) theory on project management. A descriptive survey design was adopted. A combination of purposive and simple random sampling techniques were used…

Current status of Enterobius vermicularis infection in primary schoolchildren in Miaoli County and Taichung County, Taiwan.

PubMed

Wang, Cheng-Chieh; Lee, Yuan-Fang; Chang, Chih-Cheng; Lee, Nie-Sue; Chen, Po-Yen; Huang, Fang-Liang; Liou, Nuo-Wei

2009-10-01

No epidemiological survey of the prevalence of Enterobius vermicularis infection in Miaoli County and Taichung County has been conducted. This epidemiological survey was performed to describe the profile of E. vermicularis infection in schoolchildren in Miaoli County and Taichung County. The first part of this study was conducted between November 2005 and February 2006 in Miaoli County. 44,071 primary schoolchildren from 18 districts were examined by 2 consecutive-day adhesive cellophane perianal swabs to estimate the infection rate. The second part was performed between October 2006 and January 2007 in Taichung County. 24,382 primary schoolchildren from 14 districts were examined. In Miaoli County, the infection rate was 2.39% (1054/44,071). The infection rate was highest in the Taian (6.69%; 20/299), Shytarn (4.49%; 11/245), and Dahu townships (3.6%; 40/1111). In Taichung County, the infection rate was 2.95% (720/24,382). The infection rate was highest in the Da-an (5.46%; 26/476) and Heping townships (4.48%; 9/201). The infection rate for a family with > or =3 children was significantly higher than that for a family with < or =2 children (p = 0.007). The frequency of washing linen and cleaning bedclothes significantly affected the infection rate of enterobiasis (p < 0.01). The efficacy rate for mebendazole was 96%, with no difference between 1 and 2 doses. The number of children per family and the frequency of washing linen and cleaning bedclothes were the most important factors for transmission of pinworm infection among the groups surveyed.

Water resources of Duval County, Florida

USGS Publications Warehouse

Phelps, G.G.

1994-01-01

The report describes the hydrology and water resources of Duval County, the development of its water supplies, and water use within the county. Also included are descriptions of various natural features of the county (such as topography and geology), an explanation of the hydrologic cycle, and an interpretation of the relationship between them. Ground-water and surface-water resources and principal water-quality features within the county are also discussed. The report is intended to provide the general public with an overview of the water resources Of Duval County, and to increase public awareness of water issues. Information is presented in nontechnical language to enable the general reader to understand facts about water as a part of nature, and the problems associated with its development and use.

49. Photograph of a line drawing. 'PART I, SECTION 8, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

49. Photograph of a line drawing. 'PART I, SECTION 8, BUILDINGS NO. K-1, K-3, K-5, K-7, K-9, K-10 OPENING, BUILDINGS NO. N-1 TO N-10 INCL., NAILING, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

26. Photograph of a line drawing. 'PLAN LAYOUT AND CROSS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. Photograph of a line drawing. 'PLAN LAYOUT AND CROSS SECTION OF PART I, SECTION 8, BUILDINGS NO. C-1, C-3, C-5, C-6, C-7, C-9, MIXING, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

Which Biomarker is the Best for Predicting Mortality in Incident Peritoneal Dialysis Patients: NT-ProBNP, Cardiac TnT, or hsCRP?

PubMed Central

Oh, Hyung Jung; Lee, Mi Jung; Kwon, Young Eun; Park, Kyoung Sook; Park, Jung Tak; Han, Seung Hyeok; Yoo, Tae-Hyun; Kim, Yong-Lim; Kim, Yon Su; Yang, Chul Woo; Kim, Nam-Ho; Kang, Shin-Wook

2015-01-01

Abstract Although numerous previous studies have explored various biomarkers for their ability to predict mortality in end-stage renal disease (ESRD) patients, these studies have been limited by retrospective analyses, mostly prevalent dialysis patients, and the measurement of only 1 or 2 biomarkers. This prospective study was aimed to evaluate the association between 3 biomarkers and mortality in incident 335 ESRD patients starting continuous ambulatory peritoneal dialysis (CAPD) in Korea. According to the baseline NT-proBNP, cTnT, and hsCRP levels, the patients were stratified into tertiles, and cardiovascular (CV) and all-cause mortalities were compared. Additionally, time-dependent ROC curves were constructed, and the net reclassification index (NRI) and integrated discrimination improvement (IDI) of the models with various biomarkers were calculated. We found the upper tertile of NT-proBNP was significantly associated with increased risk of both CV and all-cause mortalities. However, the upper tertile of hsCRP was significantly related only to the high risk of all-cause mortality even after adjustment for age, sex, and white blood cell counts. Moreover, NT-proBNP had the highest predictive power for CV mortality, whereas hsCRP was the best prognostic marker for all-cause mortality among these biomarkers. In conclusions, NT-proBNP is a more significant prognostic factor for CV mortality than cTnT and hsCRP, whereas hsCRP is a more significant predictor than NT-proBNP and cTnT for all-cause mortality in incident peritoneal dialysis patients. PMID:26554763

Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate.

PubMed Central

Monticello, D J; Bakker, D; Schell, M; Finnerty, W R

1985-01-01

Plasmid-borne Tn5 insertion mutants of a Pseudomonas species which accumulated 2,5-dihydroxybenzoate (gentisate) following growth on 2-hydroxybenzoate (salicylate) were obtained from a pool of mutants that were unable to grow on naphthalene. One such mutant was characterized further. The ability of this mutant to oxidize gentisate was 100-fold less than the ability of a Nah+ Sal+ strain harboring the unmutagenized plasmid, although both strains oxidized and grew on salicylate. These bacteria were presumably able to metabolize salicylate via catechol, since they possessed an inducible, plasmid-encoded catechol 2,3-dioxygenase. Our results suggest that there is an alternate, plasmid-encoded route of salicylate degradation via gentisate and that some plasmid-associated relationship between this pathway and naphthalene oxidation exists. PMID:2988437

[Prediction of total nitrogen and alkali hydrolysable nitrogen content in loess using hyperspectral data based on correlation analysis and partial least squares regression].

PubMed

Liu, Xiu-ying; Wang, Li; Chang, Qing-rui; Wang, Xiao-xing; Shang, Yan

2015-07-01

Wuqi County of Shaanxi Province, where the vegetation recovering measures have been carried out for years, was taken as the study area. A total of 100 loess samples from 24 different profiles were collected. Total nitrogen (TN) and alkali hydrolysable nitrogen (AHN) contents of the soil samples were analyzed, and the soil samples were scanned in the visible/near-infrared (VNIR) region of 350-2500 nm in the laboratory. The calibration models were developed between TN and AHN contents and VNIR values based on correlation analysis (CA) and partial least squares regression (PLS). Independent samples validated the calibration models. The results indicated that the optimum model for predicting TN of loess was established by using first derivative of reflectance. The best model for predicting AHN of loess was established by using normal derivative spectra. The optimum TN model could effectively predict TN in loess from 0 to 40 cm, but the optimum AHN model could only roughly predict AHN at the same depth. This study provided a good method for rapidly predicting TN of loess where vegetation recovering measures have been adopted, but prediction of AHN needs to be further studied.

Time constraints on the origin of large volume basalts derived from O-isotope and trace element mineral zoning and U-series disequilibria in the Laki and Grímsvötn volcanic system

NASA Astrophysics Data System (ADS)

Bindeman, Ilya N.; Sigmarsson, Olgeir; Eiler, John

2006-05-01

The 1783-1784 AD fissure eruption of Laki (Iceland) produced 15 km 3 of homogeneous basaltic lavas and tephra that are characterized by extreme (3‰) 18O-depletion relative to normal mantle. Basaltic tephra erupted over the last 8 centuries and as late as in November 2004 from the Grímsvötn central volcano, which together with Laki are a part of a single volcanic system, is indistinguishable in δ18O from Laki glass. This suggests that all tap a homogeneous and long-lived low- δ18O magma reservoir. In contrast, we observe extreme oxygen isotope heterogeneity (2.2-5.2‰) in olivine and plagioclase contained within these lavas and tephra, and disequilibrium mineral-glass oxygen-isotope fractionations. Such low- δ18O glass values, and extreme 3‰ range in δ18O olivine have not been described in any other unaltered basalt. The energy constrained mass balance calculation involving oxygen isotopes and major element composition calls for an origin of the Laki-Grímsvötn quartz tholeiitic basaltic melts with δ18O = 3.1‰ by bulk digestion of low- δ18O hydrated basaltic crust with δ18O = - 4‰ to + 1‰, rather than magma mixing with ultra-low- δ18O silicic melt. The abundant Pleistocene hyaloclastites, which were altered by synglacial meltwaters, can serve as a likely assimilant material for the Grímsvötn magmas. The ( 226Ra / 230Th) activity ratio in Laki lavas and 20th century Grímsvötn tephras is 13% in-excess of secular equilibrium, but products of the 20th century Grímsvötn eruptions have equilibrium ( 210Pb / 226Ra). Modeling of oxygen isotope exchange between disequilibrium phenocrysts and magmas, and these short-lived U-series nuclides yields a coherent age for the Laki-Grímsvötn magma reservoir between 100 and 1000 yrs. We propose the existence of uniquely fingerprinted, low- δ18O, homogeneous, large volume, and long-lived basaltic reservoir beneath the Laki-Grímsvötn volcanic system that has been kept alive in its position above the

TN International and ITS operational feedback regarding the decommissioning of obsolete casks dedicated to the transport and/or storage of nuclear raw materials, fuel and used fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Blachet, L.; Bimet, F.; Rennesson, N.

2008-07-01

Within the AREVA group, TN International is a major actor regarding the design of casks and transportation for the nuclear cycle. In the early 2005, TN International has started the project of decommissioning some of its own equipment and was hence the first company ever in the AREVA Group to implement this new approach. In order to do so, TN International has based this project by taking into account the AREVA Sustainable Development Charter, the French regulatory framework, the ANDRA (Agence Nationale pour la Gestion des Dechets Radioactifs - National Agency for the radioactive waste management) requirements and has deployedmore » a step by step methodology such as radiological characterization following a logical route. The aim was to define a standardized process with optimized solutions regarding the diversity of the cask's fleet. As a general matter, decommissioning of nuclear casks is a brand new field as the nuclear field is more familiar with the dismantling of nuclear facilities and/or nuclear power plant. Nevertheless existing workshops, maintenance facilities, measurements equipments and techniques have been exploited and adapted by TN International in order to turn an ambitious project into a permanent and cost-effective activity. The decommissioning of the nuclear casks implemented by TN International regarding its own needs and the French regulatory framework is formalized by several processes and is materialized for instance by the final disposal of casks as they are or in ISO container packed with cut-off casks and big bags filled with crushed internal cask equipments, etc. The first part of this paper aims to describe the history of the project that started with a specific environmental analysis which took into account the values of AREVA as regards the Sustainable Development principles that were at the time and are still a topic of current concern in the world. The second part will deal with the definition, the design and the implementation of

4. Close view of the logs joining of the north ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Close view of the logs joining of the north and south rooms, with scale (Note: hole with remains of peg SITU as seen in the east cabin) - The Hermitage, Alfred's Cabin, 4580 Rachel's Lane, Hermitage, Davidson County, TN

Somerset County Flood Information System

USGS Publications Warehouse

Hoppe, Heidi L.

2007-01-01

The timely warning of a flood is crucial to the protection of lives and property. One has only to recall the floods of August 2, 1973, September 16 and 17, 1999, and April 16, 2007, in Somerset County, New Jersey, in which lives were lost and major property damage occurred, to realize how costly, especially in terms of human life, an unexpected flood can be. Accurate forecasts and warnings cannot be made, however, without detailed information about precipitation and streamflow in the drainage basin. Since the mid 1960's, the National Weather Service (NWS) has been able to forecast flooding on larger streams in Somerset County, such as the Raritan and Millstone Rivers. Flooding on smaller streams in urban areas was more difficult to predict. In response to this problem the NWS, in cooperation with the Green Brook Flood Control Commission, installed a precipitation gage in North Plainfield, and two flash-flood alarms, one on Green Brook at Seeley Mills and one on Stony Brook at Watchung, in the early 1970's. In 1978, New Jersey's first countywide flood-warning system was installed by the U.S. Geological Survey (USGS) in Somerset County. This system consisted of a network of eight stage and discharge gages equipped with precipitation gages linked by telephone telemetry and eight auxiliary precipitation gages. The gages were installed throughout the county to collect precipitation and runoff data that could be used to improve flood-monitoring capabilities and flood-frequency estimates. Recognizing the need for more detailed hydrologic information for Somerset County, the USGS, in cooperation with Somerset County, designed and installed the Somerset County Flood Information System (SCFIS) in 1990. This system is part of a statewide network of stream gages, precipitation gages, weather stations, and tide gages that collect data in real time. The data provided by the SCFIS improve the flood forecasting ability of the NWS and aid Somerset County and municipal agencies in

77 FR 73005 - Revisions to the California State Implementation Plan, Imperial County, Placer County, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-07

...EPA is proposing to approve revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD) and Ventura County Air Pollution Control District (PCAPCD) portions of the California State Implementation Plan (SIP). Under authority of the Clean Air Act as amended in 1990 (CAA or the Act), we are proposing to approve local rules that address emission statements for ICAPCD and PCAPCD and definitions for VCAPCD.

The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer

PubMed Central

Munkley, Jennifer; Oltean, Sebastian; Vodák, Daniel; Wilson, Brian T.; Livermore, Karen E.; Zhou, Yan; Star, Eleanor; Floros, Vasileios I.; Johannessen, Bjarne; Knight, Bridget; McCullagh, Paul; McGrath, John; Crundwell, Malcolm; Skotheim, Rolf I.; Robson, Craig N.; Leung, Hing Y.; Harries, Lorna W.; Rajan, Prabhakar; Mills, Ian G.; Elliott, David J.

2015-01-01

Patterns of glycosylation are important in cancer, but the molecular mechanisms that drive changes are often poorly understood. The androgen receptor drives prostate cancer (PCa) development and progression to lethal metastatic castration-resistant disease. Here we used RNA-Seq coupled with bioinformatic analyses of androgen-receptor (AR) binding sites and clinical PCa expression array data to identify ST6GalNAc1 as a direct and rapidly activated target gene of the AR in PCa cells. ST6GalNAc1 encodes a sialytransferase that catalyses formation of the cancer-associated sialyl-Tn antigen (sTn), which we find is also induced by androgen exposure. Androgens induce expression of a novel splice variant of the ST6GalNAc1 protein in PCa cells. This splice variant encodes a shorter protein isoform that is still fully functional as a sialyltransferase and able to induce expression of the sTn-antigen. Surprisingly, given its high expression in tumours, stable expression of ST6GalNAc1 in PCa cells reduced formation of stable tumours in mice, reduced cell adhesion and induced a switch towards a more mesenchymal-like cell phenotype in vitro. ST6GalNAc1 has a dynamic expression pattern in clinical datasets, being significantly up-regulated in primary prostate carcinoma but relatively down-regulated in established metastatic tissue. ST6GalNAc1 is frequently upregulated concurrently with another important glycosylation enzyme GCNT1 previously associated with prostate cancer progression and implicated in Sialyl Lewis X antigen synthesis. Together our data establishes an androgen-dependent mechanism for sTn antigen expression in PCa, and are consistent with a general role for the androgen receptor in driving important coordinate changes to the glycoproteome during PCa progression. PMID:26452038

Low-Wage Counties Face Locational Disadvantages.

ERIC Educational Resources Information Center

Gibbs, Robert; Cromartie, John B.

2000-01-01

Small populations and remoteness are the most salient features of low-wage counties. These locational attributes coincide with fewer high-wage jobs, yet low wages within industries define low-wage counties more than industry composition. Although adults in low-wage counties have less education and labor force participation overall, the role played…

Strategy for Promoting the Equitable Development of Basic Education in Underdeveloped Counties as Seen from Cili County

ERIC Educational Resources Information Center

Shihua, Peng; Rihui, Tan

2009-01-01

Employing statistical analysis, this study has made a preliminary exploration of promoting the equitable development of basic education in underdeveloped counties through the case study of Cili county. The unequally developed basic education in the county has been made clear, the reasons for the inequitable education have been analyzed, and,…

Water resources of Carbon County, Wyoming

USGS Publications Warehouse

Bartos, Timothy T.; Hallberg, Laura L.; Mason, Jon P.; Norris, Jodi R.; Miller, Kirk A.

2006-01-01

Carbon County is located in the south-central part of Wyoming and is the third largest county in the State. A study to describe the physical and chemical characteristics of surface-water and ground-water resources in Carbon County was conducted by the U.S. Geological Survey in cooperation with the Wyoming State Engineer's Office. Evaluations of streamflow and stream-water quality were limited to analyses of historical data and descriptions of previous investigations. Surface-water data were not collected as part of the study. Forty-five ground-water-quality samples were collected as part of the study and the results from an additional 618 historical ground-water-quality samples were reviewed. Available hydrogeologic characteristics for various aquifers in hydrogeologic units throughout the county also are described. Flow characteristics of streams in Carbon County vary substantially depending on regional and local basin char-acteristics and anthropogenic factors. Precipitation in the county is variable with high mountainous areas receiving several times the annual precipitation of basin lowland areas. For this reason, streams with headwaters in mountainous areas generally are perennial, whereas most streams in the county with headwaters in basin lowland areas are ephemeral, flowing only as a result of regional or local rainfall or snowmelt runoff. Flow characteristics of most perennial streams are altered substantially by diversions and regulation. Water-quality characteristics of selected streams in and near Carbon County during water years 1966 through 1986 varied. Concentrations of dissolved constituents and suspended sediment were smallest at sites on streams with headwaters in mountainous areas because of resistant geologic units, large diluting streamflows, and increased vegetative cover compared to sites on streams with headwaters in basin lowlands. Both water-table and artesian conditions occur in aquifers within the county. Shallow ground water is

Immunization of breast cancer patients using a synthetic sialyl-Tn glycoconjugate plus Detox adjuvant.

PubMed

MacLean, G D; Reddish, M; Koganty, R R; Wong, T; Gandhi, S; Smolenski, M; Samuel, J; Nabholtz, J M; Longenecker, B M

1993-01-01

We have synthesized various formulations that have potential for active specific immunotherapy (ASI) of human cancers. Sialyl-Tn (STn) is a potentially important target structure for ASI because its expression on mucins is a strong, independent predictor of poor prognosis, suggesting that it may have functional significance in the metastatic process. In this first pilot study of synthetic sialyl-Tn hapten conjugated to keyhole limpet hemocyanin (STn-KLH), with Detox adjuvant, toxicity and humoral immunogenicity were assessed in 12 patients with metastatic breast cancer. Toxicity was minimal, restricted to local cutaneous reactions (apart from transient nausea and vomiting following single low-dose cyclophosphamide treatment). Using STn-conjugated human serum albumin in a solid-phase enzyme-linked immunosorbent assay, it was shown that all patients developed IgM and IgG specific for the synthetic STn hapten. Following immunization, most patients were shown to develop increased titres of complement-mediated cytotoxic antibodies, partially inhibited by synthetic STn hapten, but not by the related TF hapten. We also detected IgM and IgG antibodies reactive with natural STn determinants expressed on ovine submaxillary mucin, the STn specificity of this reactivity being confirmed by hapten inhibition. Evaluation of clinical efficacy in a small pilot study is difficult. Five patients are alive 12 or more months after entry, and another 4 patients are alive 6 or more months after entry into the study. All 3 patients with known widespread bulky disease progressed despite ASI, 2 having died from widespread cancer. Two patients had partial responses, each lasting 6 months. While several patients had disease stability for 3-10 months, 1 patient with pulmonary metastases remains stable 15 months after entry into the program.

The Economic Impact of Johnson County Community College on Johnson County, 1993-94.

ERIC Educational Resources Information Center

Johnson County Community Coll., Overland Park, KS. Office of Institutional Research.

In 1995, Johnson County Community College (JCCC), in Kansas, conducted a study to estimate the economic benefit contributed by the college to the Johnson County economy for 1993-94. Following a model developed by J. G. Ryan, direct economic impact was assessed by examining institutional expenditures, expenditures by college employees, and…

24. Historic American Buildings Survey, W. Jeter Eason Deputy District ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. Historic American Buildings Survey, W. Jeter Eason- Deputy District Officer, Photographer November 21, 1936 PLASTER ORNAMENT ON CEILING AND PLASTER CORNICE BACK PARLOR (FRONT PARLOR SIMLAR). - The Hermitage, U.S. Highway 70 North (4580 Rachel's Lane), Nashville, Davidson County, TN

21st Century jobs initiative - Tennessee`s Resource Valley. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1996-12-23

Tennessee`s Resource Valley, a regional economic development organization, was asked to facilitate a two-year, $750,000 grant from the Department of Energy. The grant`s purpose was to make the East Tennessee region less dependent on federal funds for its economic well-being and to increase regional awareness of the advantages of proximity to the Department of Energy facilities in Oak Ridge. The mission of Tennessee`s Resource Valley is to market the business location advantages of mid-East Tennessee to corporate decision makers and to facilitate regional initiatives that impact the creation of quality job opportunities. Tennessee`s Resource Valley represents fifteen (15) counties inmore » East Tennessee: Anderson, Blount, Campbell, Claiborne, Cocke, Grainger, Jefferson, Knox, Loudon, Monroe, Morgan, Roane, Scott, Sevier and Union.« less

21st Century jobs initiative - Tennessee`s Resource Valley. Progress report 1995

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-12-20

Tennessee`s Resource Valley, a regional economic development organization, was asked to facilitate a two-year, $750,000 grant from the Department of Energy. The grant`s purpose is to make the East Tennessee region less dependent on federal funds for its economic well-being and to increase regional awareness of the advantages of proximity to the Department of Energy facilities in Oak Ridge. Tennessee`s Resource Valley`s mission is to market the mid-East Tennessee region`s business location advantages to corporate decision makers and to facilitate regional initiatives that impact the creation of quality job opportunities. Tennessee`s Resource Valley represents the following fifteen (15) counties inmore » East Tennessee: Anderson, Blount, Campbell, Claiborne, Cocke, Grainger, Jefferson, Knox, Loudon, Monroe, Morgan, Scott, Sevier, and Union.« less

Forest statistics for Central Alabama counties

Treesearch

Arnold Hedlund; J.M. Earles

1972-01-01

This report tabulates information from a new forest inventory of counties in central Alabama. The tables are intended for use as source data in compiling estimates for groups of counties. Because the sampling procedure is designed primarily to furnish inventory data for the State as a whole, estimates for individual counties have limited and variable accuracy.

Forest statistics for Southwest Alabama counties

Treesearch

Arnold Hedlund; J. M. Earles

1972-01-01

This report tabulates information from a new forest inventory of counties in southwestern Alabama. The tables are intended for sue as source data in compiling estimates for groups of counties. Because the sampling procedure is designed primarily to furnish inventory data for the State as a whole, estimates for individual counties have limited and variable accuracy....

Forest statistics for North Alabama counties

Treesearch

Arnold Hedlund; J. M. Earles

1972-01-01

This report tabulates information from a new forest inventory of counties in northern Alabama. The tables are intended for use as a source data in compiling estimates for groups of counties. Because the sampling procedure is designed primarily to furnish inventory data for the state as a whole, estimates for individual counties have limited and variable accuracy.

Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites.

PubMed

Jia, Xianbo; Lin, Xinjian; Chen, Jichen

2017-11-02

Current genome walking methods are very time consuming, and many produce non-specific amplification products. To amplify the flanking sequences that are adjacent to Tn5 transposon insertion sites in Serratia marcescens FZSF02, we developed a genome walking method based on TAIL-PCR. This PCR method added a 20-cycle linear amplification step before the exponential amplification step to increase the concentration of the target sequences. Products of the linear amplification and the exponential amplification were diluted 100-fold to decrease the concentration of the templates that cause non-specific amplification. Fast DNA polymerase with a high extension speed was used in this method, and an amplification program was used to rapidly amplify long specific sequences. With this linear and exponential TAIL-PCR (LETAIL-PCR), we successfully obtained products larger than 2 kb from Tn5 transposon insertion mutant strains within 3 h. This method can be widely used in genome walking studies to amplify unknown sequences that are adjacent to known sequences.

Youth Representation on County Government Committees: Youth in Governance in Kenosha County, Wisconsin

ERIC Educational Resources Information Center

Calvert, Matthew; de Montmollin, John; Winnett, Tedi

2015-01-01

The Kenosha County Youth in Governance program was created to build leadership skills and civic engagement opportunities for high school-aged students by placing two youth representatives on each of the Kenosha County Board of Supervisors standing committees. In reviewing data from 3 years of youth participants, the program was effective in…

Dissemination and Persistence of blaCTX-M-9 Are Linked to Class 1 Integrons Containing CR1 Associated with Defective Transposon Derivatives from Tn402 Located in Early Antibiotic Resistance Plasmids of IncHI2, IncP1-α, and IncFI Groups

PubMed Central

Novais, Ângela; Cantón, Rafael; Valverde, Aránzazu; Machado, Elisabete; Galán, Juan-Carlos; Peixe, Luísa; Carattoli, Alessandra; Baquero, Fernando; Coque, Teresa M.

2006-01-01

This study analyzes the diversity of In60, a class 1 integron bearing CR1 and containing blaCTX-M-9, and its association with Tn402, Tn21, and classical conjugative plasmids among 45 CTX-M-9-producing clinical strains (41 Escherichia coli strains, 2 Klebsiella pneumoniae strains, 1 Salmonella enterica strain, and 1 Enterobacter cloacae strain). Forty-five patients in a Spanish tertiary care hospital were studied (1996 to 2003). The diversity of In60 and association of In60 with Tn402 or mercury resistance transposons were investigated by overlapping PCR assays and/or hybridization. Plasmid characterization included comparison of restriction fragment length polymorphism patterns and determination of incompatibility group by PCR-based replicon typing, sequencing, and hybridization. CTX-M-9 plasmids belonged to IncHI2 (n = 26), IncP-1α (n = 10), IncFI (n = 4), and IncI (n = 1) groups. Genetic platforms containing blaCTX-M-9 were classified in six types in relation to the In60 backbone and in eight subtypes in relation to Tn402 derivatives. They were associated with Tn21 sequences when located in IncP-1α or IncHI2 plasmids. Our study identified blaCTX-M-9 in a high diversity of CR1-bearing class 1 integrons linked to different Tn402 derivatives, often to Tn21, highlighting the role of recombination events in the evolution of antibiotic resistance plasmids. The presence of blaCTX-M-9 on broad-host-range IncP-1α plasmids might contribute to its dissemination to hosts that were not members of the family Enterobacteriaceae. PMID:16870767

Hancock County Awards Gala

NASA Image and Video Library

2008-08-26

Gene Goldman (left), deputy director of NASA's John C. Stennis Space Center, accepts an Award of Excellence from Jack Zink, executive director of the Hancock County Port and Harbor Commission, during the 2008 Annual Hancock County Awards Gala. The Award of Excellence was presented to recognize Stennis Space Center's contribution to NASA's 50 years of excellence in space exploration.

Commodity Flow Study - Clark County, Nevada, USA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Conway, S.Ph.D.; Navis, I.

2008-07-01

The United States Department of Energy has designated Clark County, Nevada as an 'Affected Unit of Local Government' due to the potential for impacts by activities associated with the Yucca Mountain High Level Nuclear Waste Repository project. Urban Transit, LLC has led a project team of transportation including experts from the University of Nevada Las Vegas Transportation Research Center to conduct a hazardous materials community flow study along Clark County's rail and truck corridors. In addition, a critical infrastructure analysis has also been carried out in order to assess the potential impacts of transportation within Clark County of high levelmore » nuclear waste and spent nuclear fuel to a proposed repository 90 miles away in an adjacent county on the critical infrastructure in Clark County. These studies were designed to obtain information relating to the transportation, identification and routing of hazardous materials through Clark County. Coordinating with the United States Department of Energy, the U.S. Department of Agriculture, the U. S. Federal Highway Administration, the Nevada Department of Transportation, and various other stakeholders, these studies and future research will examine the risk factors along the entire transportation corridor within Clark County and provide a context for understanding the additional vulnerability associated with shipping spent fuel through Clark County. (authors)« less

Volcanic ash leaching as a means of tracing the environmental impact of the 2011 Grímsvötn eruption, Iceland.

PubMed

Cabré, J; Aulinas, M; Rejas, M; Fernandez-Turiel, J L

2016-07-01

The Grímsvötn volcanic eruption, from 21 to 28 May, 2011, was the largest eruption of the Grímsvötn Volcanic System since 1873, with a Volcanic Explosivity Index (VEI) of magnitude 4. The main geochemical features of the potential environmental impact of the volcanic ash-water interaction were determined using two different leaching methods as proxies (batch and vertical flow-through column experiments). Ash consists of glass with minor amounts of plagioclase, clinopyroxene, diopside, olivine and iron sulphide; this latter mineral phase is very rare in juvenile ash. Ash grain morphology and size reflect the intense interaction of magma and water during eruption. Batch and column leaching tests in deionised water indicate that Na, K, Ca, Mg, Si, Cl, S and F had the highest potential geochemical fluxes to the environment. Release of various elements from volcanic ash took place immediately through dissolution of soluble salts from the ash surface. Element solubilities of Grímsvötn ash regarding bulk ash composition were <1 %. Combining the element solubilities and the total estimated mass of tephra (7.29 × 10(14) g), the total inputs of environmentally important elements were estimated to be 8.91 × 10(9) g Ca, 7.02 × 10(9) g S, 1.10 × 10(9) g Cl, 9.91 × 10(8) g Mg, 9.91 × 10(8) g Fe and 1.45 × 10(8) g P The potential environmental problems were mainly associated with the release of F (5.19 × 10(9) g).

77 FR 72968 - Revisions to the California State Implementation Plan, for Imperial County, Placer County and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-07

...EPA is taking direct final action to approve revisions to the Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD) and Ventura County Air Pollution Control District (PCAPCD) portions of the California State Implementation Plan (SIP). Under authority of the Clean Air Act as amended in 1990 (CAA or the Act), we are approving local rules that address emission statements for ICAPCD and PCAPCD and definitions for VCAPCD.

7 CFR 1220.626 - FSA county office report.

Code of Federal Regulations, 2010 CFR

2010-01-01

... CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.626 FSA county office report. The county FSA office report shall be certified as accurate and complete by the CED or designee... county summary of requests on a form provided by the Administrator, FSA. Each county FSA office shall...

7 CFR 1220.626 - FSA county office report.

Code of Federal Regulations, 2013 CFR

2013-01-01

... CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.626 FSA county office report. The county FSA office report shall be certified as accurate and complete by the CED or designee... county summary of requests on a form provided by the Administrator, FSA. Each county FSA office shall...

7 CFR 1220.626 - FSA county office report.

Code of Federal Regulations, 2014 CFR

2014-01-01

... CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.626 FSA county office report. The county FSA office report shall be certified as accurate and complete by the CED or designee... county summary of requests on a form provided by the Administrator, FSA. Each county FSA office shall...

7 CFR 1220.626 - FSA county office report.

Code of Federal Regulations, 2012 CFR

2012-01-01

... CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.626 FSA county office report. The county FSA office report shall be certified as accurate and complete by the CED or designee... county summary of requests on a form provided by the Administrator, FSA. Each county FSA office shall...

7 CFR 1220.626 - FSA county office report.

Code of Federal Regulations, 2011 CFR

2011-01-01

... CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.626 FSA county office report. The county FSA office report shall be certified as accurate and complete by the CED or designee... county summary of requests on a form provided by the Administrator, FSA. Each county FSA office shall...

Effect of the polymer concentration on the ON/OFF states of a TN-LCD: polyvinyl alcohol vs. soy lecithin

NASA Astrophysics Data System (ADS)

de Coss Martinez, Romeo; Gonzalez Murguia, Jose Luis

2011-03-01

In this work we study the response of a Twisted Nematic Liquid Crystal Display (TN-LCD) by varying both the concentration and the polymer used for the microgroove. We compare the performance of two polymers: polyvinyl alcohol and soy lecithin. In particular, the light transmission for the ON/OFF states is evaluated. The polyvinyl alcohol is a polymer widely used in LCDs while lecithin soy is a natural polymer.

Hydrogeologic framework of LaSalle County, Illinois

USGS Publications Warehouse

Kay, Robert T.; Bailey, Clinton R.

2016-10-28

Water-supply needs in LaSalle County in northern Illinois are met by surface water and groundwater. Water-supply needs are expected to increase to serve future residential and mining uses. Available information on water use, geology, surface-water and groundwater hydrology, and water quality provides a hydrogeologic framework for LaSalle County that can be used to help plan the future use of the water resources.The Illinois, Fox, and Vermilion Rivers are the primary surface-water bodies in LaSalle County. These and other surface-water bodies are used for wastewater disposal in the county. The Vermilion River is used as a drinking-water supply in the southern part of the county. Water from the Illinois and Fox Rivers also is used for the generation of electric power.Glacial drift aquifers capable of yielding sufficient water for public supply are expected to be present in the Illinois River Valley in the western part of the county, the Troy Bedrock Valley in the northwestern part of the county, and in the Ticona Bedrock Valley in the south-central part of the county. Glacial drift aquifers capable of yielding sufficient water for residential supply are present in most of the county, although well yield often needs to be improved by using large-diameter wells. Arsenic concentrations above health-based standards have been detected in some wells in this aquifer. These aquifers are a viable source for additional water supply in some areas, but would require further characterization prior to full development.Shallow bedrock deposits comprising the sandstone units of the Ancell Group, the Prairie du Chien Group, dolomite of the Galena and Platteville Groups, and Silurian-aged dolomite are utilized for water supply where these units are at or near the bedrock surface or where overlain by Pennsylvanian-aged deposits. The availability of water from the shallow bedrock deposits depends primarily on the geologic unit analyzed. All these deposits can yield sufficient water for

13. Photograph of a line drawing. 'BUILDING LOCATION PLAN, AREAS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Photograph of a line drawing. 'BUILDING LOCATION PLAN, AREAS A & B.' Holston Army Ammunition Plant, Holston Defense Corporation. September 8, 1970. Delineator: R. C. G. Drawing # 7651-212/1226.03. - Holston Army Ammunition Plant, Producer Gas Plant, Kingsport, Sullivan County, TN

10. Photograph of a line drawing. 'PROCESS FLOW SCHEMATIC, GAS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Photograph of a line drawing. 'PROCESS FLOW SCHEMATIC, GAS PRODUCER PROCESS, BUILDING 10A.' Holston Army Ammunition Plant, Holston Defense Corporation. August 29, 1974. Delineator: G. A. Horne. Drawing # SK-1942. - Holston Army Ammunition Plant, Producer Gas Plant, Kingsport, Sullivan County, TN

Providing engineering services to counties.

DOT National Transportation Integrated Search

2008-09-01

An engineer is required by law to safeguard the health, safety and welfare of the public. The current Kansas : statute state, The Board of County Commissioners of each county shall appoint a licensed professional : engineer, whose title shall be c...

Program of Education for Community Coordination and Action in Warren County: What Kind of a County Do We Want Warren County To Be?

ERIC Educational Resources Information Center

Koontz, Donald H.; Wallerstedt, Jane

A program initiated by Simpson College and assisted by a committee of representatives from Warren County, Iowa, to assist the population of the County to understand their community and its problems and set priorities in meeting these problems is reported. Two hundred key leaders, representative of the geographic, educational, economic, religious,…

Study of the performance of the infrasound Station IS48TN during the period 2011 -2017

NASA Astrophysics Data System (ADS)

Mejri, Chourouk

2017-04-01

The Infrasound Station IS48, in Kesra, Tunisia is part of the verification regime for the Comprehensive Nuclear-Test-Ban Treaty. IS48 is managed and maintained by the Tunisian NDC. Its good location in the middle of the Mediterranean Sea allows to have various and interesting detections. Several events were recorded and identified. But since 2010, the performance of the station has began to be noisy , due to a leakage in the pipe array, despite efforts to solve the issue through the pressure test and maintenance . To this issues, PTS decided to upgrade the WNRS at IS48TN.

Hydrogeology of Cibola County, New Mexico

USGS Publications Warehouse

Baldwin, J.A.; Rankin, D.R.

1995-01-01

The hydrogeology of Cibola County, New Mexico, was evaluated to determine the occurrence, availability, and quality of ground-water resources. Rocks of Precambrian through Quaternary age are present in Cibola County. Most rocks are sedimentary in origin except for Precambrian igneous and metamorphic rocks exposed in the Zuni Uplift and Tertiary and Quaternary basalts in northern and central parts of the county. The most productive aquifers in the county include (youngest to oldest) Quaternary deposits, sandstones in the Mesaverde Group, the Dakota-Zuni-Bluff aquifer, the Westwater Canyon aquifer, the Todilto- Entrada aquifer, sandstone beds in the Chinle Formation, and the San Andres-Glorieta aquifer. Unconsolidated sand, silt, and gravel form a mantle ranging from a few inches to 150 to 200 feet over much of the bedrock in Cibola County. Well yields range from 5 to 1,110 gallons per minute. Dissolved-solids concentrations of ground water range from 200 to more than 5,200 milligrams per liter. Calcium, magnesium, bicarbonate, and sulfate are the predominant ions in ground water in alluvial material. The Mesaverde Group mainly occurs in three areas of the county. Well yields range from less than 1 to 12 gallons per minute. The predominant ions in water from wells in the Mesaverde Group are calcium, sodium, and bicarbonate. The transition from calcium-predominant to sodium-predominant water in the southwestern part of the county likely is a result of ion exchange. Wells completed in the Dakota-Zuni-Bluff aquifer yield from 1 to 30 gallons per minute. Dissolved-solids concentrations range from 220 to 2,000 milligrams per liter in water from 34 wells in the western part of the county. Predominant ions in the ground water include calcium, sodium, sulfate, and bicarbonate. Calcium predominates in areas where the aquifer is exposed at the surface or is overlain with alluvium. Sandstones in the Chinle Formation yield from 10 to 300 gallons per minute to wells in the Grants

Measuring county resilience after the 2008 Wenchuan earthquake

NASA Astrophysics Data System (ADS)

Li, X.; Lam, N.; Qiang, Y.; Li, K.; Yin, L.; Liu, S.; Zheng, W.

2015-01-01

The catastrophic earthquake in 2008 has caused serious damage to Wenchuan County and the surrounding area in China. In recent years, great attention has been paid to the resilience of the affected area. This study applied a new framework, the Resilience Inference Measurement (RIM) model, to quantify and validate the community resilience of 105 counties in the affected area. The RIM model uses cluster analysis to classify counties into four resilience levels according to the exposure, damage, and recovery conditions, and then applies discriminant analysis to quantify the influence of socioeconomic characteristics on the county resilience. The analysis results show that counties located right at the epicenter had the lowest resilience, but counties immediately adjacent to the epicenter had the highest resilience capacities. Counties that were farther away from the epicenter returned to normal resiliency. The socioeconomic variables, including sex ratio, per capita GDP, percent of ethnic minority, and medical facilities, were identified as the most influential socio-economic characteristics on resilience. This study provides useful information to improve county resilience to earthquakes and support decision-making for sustainable development.

Illinois Kids Count: A Snap Shot of Our Future. County by County Profiles of Child Well-Being '92.

ERIC Educational Resources Information Center

Voices for Illinois Children, Chicago.

This booklet presents statistics concerning the well-being of Illinois' 3.3 million children between 1980 and 1990. Statistics are compared county by county for each of the state's 102 counties, and statewide statistics are compared with those of the entire nation. A statewide analysis focuses on spending per pupil on education, the percentage of…

Tri-county pilot study. [Texas

NASA Technical Reports Server (NTRS)

Reeves, C. A. (Principal Investigator); Austin, T. W.; Kerber, A. G.

1976-01-01

The author has identified the following significant results. An area inventory was performed for three southeast Texas counties (Montgomery, Walker, and San Jacinto) totaling 0.65 million hectares. The inventory was performed using a two level hierarchy. Level 1 was divided into forestland, rangeland, and other land. Forestland was separated into Level 2 categories: pine, hardwood, and mixed; rangeland was not separated further. Results consisted of area statistics for each county and for the entire study site for pine, hardwood, mixed, rangeland, and other land. Color coded county classification maps were produced for the May data set, and procedures were developed and tested.

43 CFR 2806.21 - When and how are counties or other geographical areas assigned to a County Zone Number and Per...

Code of Federal Regulations, 2012 CFR

2012-10-01

... geographical areas assigned to a County Zone Number and Per Acre Zone Value? 2806.21 Section 2806.21 Public... MANAGEMENT ACT Rents Linear Rights-Of-Way § 2806.21 When and how are counties or other geographical areas assigned to a County Zone Number and Per Acre Zone Value? Counties (or other geographical areas) are...

43 CFR 2806.21 - When and how are counties or other geographical areas assigned to a County Zone Number and Per...

Code of Federal Regulations, 2011 CFR

2011-10-01

... geographical areas assigned to a County Zone Number and Per Acre Zone Value? 2806.21 Section 2806.21 Public... MANAGEMENT ACT Rents Linear Rights-Of-Way § 2806.21 When and how are counties or other geographical areas assigned to a County Zone Number and Per Acre Zone Value? Counties (or other geographical areas) are...

43 CFR 2806.21 - When and how are counties or other geographical areas assigned to a County Zone Number and Per...

Code of Federal Regulations, 2013 CFR

2013-10-01

... geographical areas assigned to a County Zone Number and Per Acre Zone Value? 2806.21 Section 2806.21 Public... MANAGEMENT ACT Rents Linear Rights-Of-Way § 2806.21 When and how are counties or other geographical areas assigned to a County Zone Number and Per Acre Zone Value? Counties (or other geographical areas) are...

43 CFR 2806.21 - When and how are counties or other geographical areas assigned to a County Zone Number and Per...

Code of Federal Regulations, 2014 CFR

2014-10-01

... geographical areas assigned to a County Zone Number and Per Acre Zone Value? 2806.21 Section 2806.21 Public... MANAGEMENT ACT Rents Linear Rights-Of-Way § 2806.21 When and how are counties or other geographical areas assigned to a County Zone Number and Per Acre Zone Value? Counties (or other geographical areas) are...

40. Photograph of a line drawing. 'FLOW DIAGRAM FOR BUILDING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

40. Photograph of a line drawing. 'FLOW DIAGRAM FOR BUILDING G-1.' Holston Army Ammunition Plant, Holston Defense Corporation. December 1, 1965. Delineator: V. N.J. Drawing # 7651-1007-528. - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

Community Types and Mortality in Georgia Counties

ERIC Educational Resources Information Center

Young, Frank W.

2012-01-01

Using an "ecological regional analysis" methodology for defining types of communities and their associated mortality rates, this study of Georgia's 159 counties finds that the suburban and town centered counties have low mortality while the city-centered type predicts low mortality for the whites. The military-centered counties do not…

Two types of phytases (histidine acid phytase and β-propeller phytase) in Serratia sp. TN49 from the gut of Batocera horsfieldi (coleoptera) larvae.

PubMed

Zhang, Rui; Yang, Peilong; Huang, Huoqing; Shi, Pengjun; Yuan, Tiezheng; Yao, Bin

2011-11-01

Microbial phytases play a major role in the mineralization of organic phosphorous, especially in symbiotic plants and animals. In this study, we identified two types of phytases in Serratia sp. TN49 that was harbored in the gut of Batocera horsfieldi (Coleoptera) larvae. The two phytases, an acidic histidine acid phosphatase (PhyH49) and an alkaline β-propeller phytase (PhyB49), shared low identities with known phytases (61% at most). PhyH49 and PhyB49 produced in Escherichia coli exhibited maximal activities at pH 5.0 (60°C) and pH 7.5-8.0 (45°C), respectively, and are complementary in phytate degradation over the pH range 2.0-9.0. Serratia sp. TN49 harboring both PhyH49 and PhyB49 might make it more adaptive to environment change, corresponding to the evolution trend of microorganism.

IMPACT of City-County Consolidation of the Rural-Urban Fringe: Nashville-Davidson County, Tennessee.

ERIC Educational Resources Information Center

Economic Research Service (USDA), Washington, DC.

This report analyzed the effect of consolidation of city and county governments in Nashville, Tenn., on local public finance and the availability of public services in the rural areas of the county. Comparisons were made between governmental costs and functions before and 3 years after the metropolitan district was formed. Some 299 voters in the…

Constraints of Implementing Free Secondary Education in Mandera West Sub-County, Mandera County, Kenya

ERIC Educational Resources Information Center

Adan, Mohammed Abdi; Orodho, John Aluko

2015-01-01

This study sought to find out the constraints of implementing free secondary education (FSE) in secondary schools in Mandera West Sub-County, Mandera County, Kenya. The study is based on the theory of constraints as the researcher examines the factors constraining the achievement of FSE objectives. The study used the survey design. The main…

Diversity of Tn1546 in vanA-positive Enterococcus faecium clinical isolates with VanA, VanB, and VanD phenotypes and susceptibility to vancomycin.

PubMed

Cha, J O; Yoo, J I; Kim, H K; Kim, H S; Yoo, J S; Lee, Y S; Jung, Y H

2013-10-01

To investigate diversity in the vanA cluster in Enterococcus faecium isolates from nontertiary hospitals. We identified 43 vanA-positive Ent. faecium isolates, including two vancomycin-susceptible isolates, from hospitals between 2003 and 2006. Of these isolates, >85% were resistant to ampicillin, erythromycin and ciprofloxacin. The vanA cluster was classified into six types using overlapping PCR, but the prototype transposon Tn1546 was not found. Most vanA-positive vancomycin-resistant Enterococcus (VRE) carried IS1216V and belonged to Type III (58·1%) or Type II (20·9%). vanY, vanZ and IS1216V were observed in the left and right ends of Type III with long-range PCR. IS1216V was also observed within vanS and vanX in the two vancomycin-susceptible isolates and in two vancomycin-resistant isolates. No VRE isolates with VanB and VanD phenotypes contained point mutations in vanS, unlike in previous reports. Sequence types (STs) of all isolates belonged to clonal complex 17, and ST78 was predominant. Insertion sequences, especially IS1216V, cause structural variation in the vanA cluster. We report the first observation of vanY and vanZ at the left end of Tn1546 in clinical isolates. This is the first report of the frequency of vancomycin resistance and diversity of Tn1546 in vanA-positive Ent. faecium isolates from nontertiary hospitals. © 2013 The Society for Applied Microbiology.

Walking to Save a County.

ERIC Educational Resources Information Center

Slotnick, Karen

1981-01-01

Describes the 10-year history and accomplishments of the Walk to Save the County which has preserved more than 400 acres of Onondaga County, New York. Outlines organizational structure, promotional strategies, awards, and educational opportunities involved in this annual fund-raising hike by third- through eighth-grade students. (NEC)

A quantitative real-time PCR assay for the detection of tetR of Tn10 in Escherichia coli using SYBR Green and the Opticon.

PubMed

Morsczeck, Christian; Langendörfer, Daniel; Schierholz, Jörg Michael

2004-06-30

Bacteria of implant infections are extremely resistant to antibiotics. One reason for this antibiotic resistance are transposons; the well-known transposon Tn10, for example, mediates tetracycline resistance to Escherichia coli. Two genes of Tn10, tetA and tetR, are essential for the mechanism of resistance. These genes encode a drug-specific efflux protein and a tetracycline repressor protein, respectively. Tn10 is also widely used in molecular biology. For example, tTA, a recombinant derivate of tetR, has been utilised for a highly efficient gene regulation system in mammalian cells. We have examined E. coli isolates from implant infections for tetracycline resistance and for the presence of tetR. A real-time PCR assay was developed for detection of tetR with SybrGreen using the Opticon PCR machine of MJ Research. This method offers a quick, sensitive, efficient, and reliable approach to the detection and quantification of genes. Clinical isolates of E. coli were examined successfully for tetracycline resistance and for the presence of tetR. The real-time PCR is effective using a variety of templates including isolated E. coli DNA, pure colonies, or liquid culture sources. Using quantified standard DNA, this assay can accurately detect as few as 15 copies. Moreover, this assay has the ability to quantify the number of tetR genes in the presence of contaminating mammalian DNA. In conclusion, the tetR real-time PCR offers new methods for detection and quantification of tetracycline-resistant bacteria and tTA in transfected cell-lines or transgenic animals.

Agreement between Luzerne County Community College and Luzerne County Community College Association of Higher Education.

ERIC Educational Resources Information Center

Luzerne County Community Coll., Nanticoke, PA.

This agreement between the Luzerne County Community College and the Luzerne County Community College Association of Higher Education covers the 1973-74 year. The agreement includes the collective bargaining agreement, definitions, recognition, fair practices, conference, maintenance of membership, check-off, grievance procedure, rights of college,…

Survival of Escherichia coli with and without ColE1::Tn5 after aerosol dispersal in a laboratory and a farm environment.

PubMed Central

Marshall, B; Flynn, P; Kamely, D; Levy, S B

1988-01-01

The survival of a laboratory strain and a naturally occurring fecal strain of Escherichia coli, with and without a Tn5-containing derivative of ColE1, was examined after aerosol dispersal in a laboratory office and a barn under ambient temperature and humidity conditions. Following the release of paired strains, air and diverse types of surfaces were assayed for the test organisms. In both environments, the number of airborne bacteria declined rapidly within the first 2 h. Longer survival was found on surfaces and varied with surface type: recovery was greatest from wood products. Organisms persisted for 1 day in the office and for up to 20 days in the barn. Survival of the fecal strain was better than that of the laboratory strain in both test environments. In general, plasmid-bearing strains fared similarly to their plasmidless parents, but in several comparisons the ColE1::Tn5-containing strain showed enhanced survival. These studies have implications for the present and proposed release of genetically engineered organisms with and without plasmid vectors. PMID:2843099

Correct county areas with sidebars for Virginia

Treesearch

Joseph M. McCollum; Dale Gormanson; John Coulston

2009-01-01

Historically, Forest Inventory and Analysis (FIA) has processed field inventory data at the county level and county estimates of land area were constrained to equal those reported by the Census Bureau. Currently, the Southern Research Station FIA unit processes field inventory data at the survey unit level (groups of counties with similar ecological characteristics)....

Evaluation of a county enforcement program with a primary seat belt ordinance : St. Louis County, Missouri.

DOT National Transportation Integrated Search

2010-05-01

In March 2007, St. Louis County implemented a seat belt ordinance that allowed for traditional : enforcement procedures. In order to increase usage on St. Louis County roads, particularly on roadways : with fatal or disabling injury crashes, the St. ...

Conformational organizations of G-quadruplexes composed of d(G(4)T(n))(3)G(4).

PubMed

Wong, Wan Chi; Zhuang, Jinyi; Ng, Selina Ling Ling; New, Lilian Li Lin; Hiew, Shuhui; Guo, Juanjuan; Yang, Zhaoqi; Li, Tianhu

2010-08-01

Structural polymorphism is one of the important issues with regard to G-quadruplexes because the structural diversity may significantly affect their biological functions in vivo and their physical property in nano-material. A series of oligonucleotides with four repeat guanines sequence [d(G(4)T(n))(3)G(4) (n=1-6)] were designed. In this study, the effects of loop length on the formation of structures of G-quadruplex were investigated through the result of CD (circular dichroism) and 20% non-denatured polyacrylamide gel electrophoresis. Our studies demonstrate that the length of loop in 100mM KCl solution could predict the conformation of G-quadruplex. Copyright 2010 Elsevier Ltd. All rights reserved.

The flora of Oktibbeha County, Mississippi

USGS Publications Warehouse

Leidolf, A.; McDaniel, S.; Nuttle, T.

2002-01-01

We surveyed the flora of Oktibbeha County, Mississippi, U.S.A., from February 1994 to 1996. Occupying 118 square kilometers in east-central Mississippi, Oktibbeha County lies among 3 physiographic regions that include, from west to east, Interior Flatwoods, Pontotoc Ridge, and Black Prairie. Accordingly, the county harbors a diverse flora. Based on field work, as well as an extensive review of published literature and herbarium records at IBE and MISSA, we recorded a total of 1,148 taxa (1,125 species, 7 hybrids, 16 infraspecific taxa) belonging to 514 genera in 160 families, over 85% of all taxa documented were native. Compared to 3 other counties in east-central Mississippi, Oktibbeha County has the second largest recorded flora. The number of state-listed (endangered, threatened, or of special concern) taxa (67) documented in this survey far exceeds that reported from any other county in the region. Three introduced species, Ilex cornuta Lindl. & Paxton, Mahonia bealei (Fortune) Carrie??re, and Nandina domestica Thunb., are reported in a naturalized state for the first time from Mississippi. We also describe 16 different plant communities belonging to 5 broad habitat categories: bottomland forests, upland forests and prairies, aquatic habitats, seepage areas, and human-influenced habitats. A detailed description of the vegetation associated with each of these communities is provided.

Trouble Brewing in Orange County. Policy Brief

ERIC Educational Resources Information Center

Buck, Stuart

2010-01-01

Orange County will soon face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that Orange County faces a total $41.2 billion liability for retiree benefits that are underfunded--including $9.4 billion for the county pension system and an estimated…

Case Study: Hunterdon County Polytech Career Academy.

ERIC Educational Resources Information Center

Southern Regional Education Board (SREB), 2012

2012-01-01

At first glance, Hunterdon County Polytech Career Academy (HCP) in Flemington, New Jersey, looks like a dream school. This shared-time career academy is an autonomous school district located in Hunterdon County--a county with one of the highest per-capita incomes in the United States--and is 60 miles from both New York City and Philadelphia. HCP…

Tn-seq of Caulobacter crescentus under uranium stress reveals genes essential for detoxification and stress tolerance

DOE PAGES

Yung, Mimi C.; Park, Dan M.; Overton, K. Wesley; ...

2015-07-20

Ubiquitous aquatic bacterium Caulobacter crescentus is highly resistant to uranium (U) and facilitates U biomineralization and thus holds promise as an agent of U bioremediation. In order to gain an understanding of how C. crescentus tolerates U, we employed transposon (Tn) mutagenesis paired with deep sequencing (Tn-seq) in a global screen for genomic elements required for U resistance. Of the 3,879 annotated genes in the C. crescentus genome, 37 were found to be specifically associated with fitness under U stress, 15 of which were subsequently tested through mutational analysis. Systematic deletion analysis revealed that mutants lacking outer membrane transporters (rsaFamore » and rsaFb), a stress-responsive transcription factor (cztR), or a ppGpp synthetase/hydrolase (spoT) exhibited a significantly lower survival rate under U stress. RsaFa and RsaFb, which are homologues of TolC in Escherichia coli, have previously been shown to mediate S-layer export. Transcriptional analysis revealed upregulation of rsaF a and rsaF b by 4- and 10-fold, respectively, in the presence of U. We additionally show that rsaFa mutants accumulated higher levels of U than the wild type, with no significant increase in oxidative stress levels. These results suggest a function for RsaF a and RsaF b in U efflux and/or maintenance of membrane integrity during U stress. In addition, we present data implicating CztR and SpoT in resistance to U stress. Together, our findings reveal novel gene targets that are key to understanding the molecular mechanisms of U resistance in C. crescentus.« less

Topics for Lehigh County Seniors.

ERIC Educational Resources Information Center

Lehigh County Community Coll., Schnecksville, PA.

Lehigh County Community College (Pennsylvania) and the Lehigh County Senior Citizens' Center collaborated on a project to assess the learning needs of the senior center's 1,600 members. A needs assessment completed by 68 center members and interviews of an additional 38 center members established that senior citizens preferred short-term workshops…

2012 ESTCP Live Site Demonstrations, Spencer, TN. ESTCP MR-1165 Demonstration Data Report, Former Spencer Artillery Range TEMTADS Demonstration

DTIC Science & Technology

2013-05-29

Victoria Kantsios, 2 Glenn R. Harbaugh,3 and Daniel A. Steinhurst3 Naval Research Laboratory, Code 6110 4555 Overlook Avenue, SW Washington, DC 20375...Unclassified Unlimited 72 B.J. Spargo, NRL, Code 6110 (202) 404-6392 The TEMTADS 5×5 Array and TEMTADS MP 2 × 2 Cart were demonstrated at the former...Naval Research Laboratory Washington, DC 20375-5320 NRL/MR/ 6110 --13-9470 2012 ESTCP Live Site Demonstrations Spencer, TN ESTCP MR-1165 Demonstration

Drug Abuse Montgomery County Pennsylvania.

ERIC Educational Resources Information Center

Montgomery County Drug Commission, Norristown, PA.

This is a research report and survey on drug abuse in Montgomery County, Norristown, Pennsylvania, conducted by the Montgomery County Drug Commission. The nine-month study is incorporated into a single volume. An analysis of the results of the drug survey points out that many variables which had heretofore been regarded as being significantly…

County portraits of Oregon and Northern California.

Treesearch

Wendy J. McGinnis; Richard H. Phillips; Kent P. Connaughton

1996-01-01

This publication provides a general picture of the population, economy, and natural resources of the counties in Oregon and northern California. The intent of this report is to provide insight to changes in a county over the last 10 to 20 years, to compare county trends to statewide trends (and state trends to national trends), and to provide information on all the...

Final Scientific/Technical Report for DOE/EERE Comprehensive Community Renewable Energy Implementation Plan in Forest County and Milwaukee County

DOE Office of Scientific and Technical Information (OSTI.GOV)

Karman, Nathan

2014-06-27

Forest County Potawatomi Community (the “Community”) sought and obtained Community Renewable Energy Deployment funding from the Department of Energy to evaluate and implement a diverse number of renewable energy technologies throughout its lands held in trust or owned in fee simple in Forest County and Milwaukee County (the “Project”). The technologies and sites evolved during the Project, ultimately leading to the investigation of biomass and solar projects on the Community’s reservation in Forest County, as well as the investigation and eventual deployment of a solar project and an anaerobic digestion and biogas project on Community lands in Milwaukee.

76 FR 19970 - Madera County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-11

... DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Madera County Resource Advisory Committee will be... assign project monitoring responsibilities for the remainder of the year. The Madera County Resource...

Self-reported physical activity in a rural county: a New York county health census.

PubMed Central

Eaton, C B; Nafziger, A N; Strogatz, D S; Pearson, T A

1994-01-01

OBJECTIVES. Few studies have described physical activity in rural populations. This study describes the frequency, types, and correlates of physical activity in 29,304 free-living adults in a rural county in New York State. METHODS. Self-reported responses about regular physical activity (maintained long enough to work up a sweat) were analyzed from a private household census of Otsego County with an 86.6% response rate. RESULTS. This survey categorized 46.2% of county residents as sedentary. Walking, the most frequent choice of activity (62% of the women, 36% of the men), increased in frequency with age of respondents whereas cycling, jogging, aerobics, team sports, and swimming (listed in rank order of frequency) generally tended to decrease in frequency with age. Farmers demonstrated an increased amount of "sweat activity" compared with persons in most other occupations. CONCLUSIONS. This descriptive study of physical activity in a rural county shows that sedentary lifestyle is of high prevalence. The high frequency of walking and the gender differences in both the levels and choice of activity suggest that further research and public policy recommendations focus on these issues. PMID:8279607

Do US metropolitan core counties have lower scope 1 and 2 CO2 emissions than less urbanized counties?

NASA Astrophysics Data System (ADS)

Tamayao, M. M.; Blackhurst, M. F.; Matthews, H. S.

2014-10-01

Recent sustainability research has focused on urban systems given their high share of environmental impacts and potential for centralized impact mitigation. Recent research emphasizes descriptive statistics from place-based case studies to argue for policy action. This limits the potential for general insights and decision support. Here, we implement generalized linear and multiple linear regression analyses to obtain more robust insights on the relationship between urbanization and greenhouse gas (GHG) emissions in the US We used consistently derived county-level scope 1 and scope 2 GHG inventories for our response variable while predictor variables included dummy-coded variables for county geographic type (central, outlying, and nonmetropolitan), median household income, population density, and climate indices (heating degree days (HDD) and cooling degree days (CDD)). We find that there is not enough statistical evidence indicating per capita scope 1 and 2 emissions differ by geographic type, ceteris paribus. These results are robust for different assumed electricity emissions factors. We do find statistically significant differences in per capita emissions by sector for different county types, with transportation and residential emissions highest in nonmetropolitan (rural) counties, transportation emissions lowest in central counties, and commercial sector emissions highest in central counties. These results indicate the importance of regional land use and transportation dynamics when planning local emissions mitigation measures.

Association of Genome-Wide Variation with Highly Sensitive Cardiac Troponin-T (hs-cTnT) Levels in European- and African-Americans: A Meta-Analysis from the Atherosclerosis Risk in Communities and the Cardiovascular Health Studies

PubMed Central

Yu, Bing; Barbalic, Maja; Brautbar, Ariel; Nambi, Vijay; Hoogeveen, Ron C.; Tang, Weihong; Mosley, Thomas H.; Rotter, Jerome I.; deFilippi, Christopher R.; O’Donnell, Christopher J.; Kathiresan, Sekar; Rice, Ken; Heckbert, Susan R.; Ballantyne, Christie M.; Psaty, Bruce M.; Boerwinkle, Eric

2013-01-01

Background High levels of cardiac troponin T measured by a highly sensitive assay (hs-cTnT) are strongly associated with incident coronary heart disease (CHD) and heart failure (HF). No large-scale genome-wide association study (GWAS) of hs-cTnT has been reported to date. We sought to identify novel genetic variants that are associated with hs-cTnT levels. Methods and Results We performed a GWAS in 9,491 European-Americans and 2,053 African-Americans free of CHD and HF from 2 prospective cohorts: the Atherosclerosis Risk in Communities Study (ARIC) and the Cardiovascular Health Study (CHS). GWASs were conducted in each study and race stratum. Fixed-effect meta-analyses combined the results of linear regression from 2 cohorts within each race stratum, and then across race strata to produce overall estimates and p-values. The meta-analysis identified a significant association at chromosome 8q13 (rs10091374, p = 9.06 × 10−9) near the nuclear receptor coactivator 2 (NCOA2) gene. Over-expression of NCOA2 can be detected in myoblasts An additional analysis using logistic regression and the clinically motivated 99th percentile cut-point detected a significant association at 1q32 (rs10091374, p = 9.06 × 10−8) in the gene TNNT2, which encodes the cardiac troponin T protein itself. The hs-cTnT-associated SNPs were not associated with CHD in a large case-control study, but rs12564445 was significantly associated with incident HF in ARIC European-Americans (HR = 1.16, p-value = 0.004). Conclusions We identified 2 loci, near NCOA2 and in the TNNT2 gene, at which variation was significantly associated with hs-cTnT levels. Further use of the new assay should enable replication of these results. PMID:23247143

Hydrogeology of Webb County, Texas

USGS Publications Warehouse

Lambert, Rebecca B.

2004-01-01

IntroductionWebb County, in semiarid South Texas on the U.S.-Mexico border, is a region confronted by increasing stresses on natural resources. Laredo (fig. 1), the largest city in Webb County (population 193,000 in 2000), was one of the 10 fastest-growing metropolitan areas in the country during 1990-2000 (Perry and Mackun, 2001). Commercial and industrial activities have expanded throughout the region to support the maquiladora industry (manufacturing plants in Mexico) along the border and other growth as a result of the passage of the North American Free Trade Agreement. The Rio Grande currently (2002) is the primary source of public water supply for Laredo and other cities along the border in Webb County (fig. 1). Other cities, such as Bruni and Mirando City in the southeastern part of the county, rely on ground-water supplies to meet municipal demands. Increased water demand associated with development and population growth in the region has increased the need for the City of Laredo and Webb County to evaluate alternative water sources to meet future demand. Possible options include (1) supplementing the surface-water supply with ground water, and (2) applying artificial storage and recovery (ASR) technology to recharge local aquifers. These options raise issues regarding the hydraulic capability of the aquifers to store economically substantial quantities of water, current or potential uses of the resource, and possible effects on the quality of water resulting from mixing ground water with alternative source waters. To address some of these issues, the U.S. Geological Survey (USGS), in cooperation with the City of Laredo, began a study in 1996 to assess the ground-water resources of Webb County. A hydrogeologic study was conducted to review and analyze available information on the hydrogeologic units (aquifers and confining units) in Webb County, to locate available wells in the region with water-level and water-quality information from the aquifers, and to

75 FR 31759 - Madera County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-04

... DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Madera County Resource Advisory Committee will be... Road 225, North Fork, California 93643. Send written comments to Julie Roberts, Madera County Resource...

Selected hydrologic data for the Beaver Dam Wash area, Washington County, Utah, Lincoln County, Nevada, and Mohave County, Arizona, 1991-95

USGS Publications Warehouse

Enright, Michael

1996-01-01

The hydrologic data in this report were collected in Beaver Dam Wash and adjacent areas of Washington County, Utah, Lincoln County, Nevada, andMohave County, Arizona, from 1991 to 1995; some historical data from as far back as 1932 are included for comparative purposes. The data include records of about 100 wells, drillers' and geologic logs of selected wells, and results of chemical analyses of water from wells, springs, and surface-water sites. Discharge, water temperature, and specific-conductance measurements are reported for 33 surface-water and spring sites. Daily mean discharge data are reported for two U.S. Geological Survey streamflow-gaging stations on Beaver Dam Wash (1992-95). The data were collected as part of a study done by the U.S. Geological Survey in cooperation with the Utah Department of Natural Resources, Division of Water Resources; the Nevada Department of Conservation and Natural Resources; and the Arizona Department of Water Resources.

Counties eliminating racial disparities in colorectal cancer mortality.

PubMed

Rust, George; Zhang, Shun; Yu, Zhongyuan; Caplan, Lee; Jain, Sanjay; Ayer, Turgay; McRoy, Luceta; Levine, Robert S

2016-06-01

Although colorectal cancer (CRC) mortality rates are declining, racial-ethnic disparities in CRC mortality nationally are widening. Herein, the authors attempted to identify county-level variations in this pattern, and to characterize counties with improving disparity trends. The authors examined 20-year trends in US county-level black-white disparities in CRC age-adjusted mortality rates during the study period between 1989 and 2010. Using a mixed linear model, counties were grouped into mutually exclusive patterns of black-white racial disparity trends in age-adjusted CRC mortality across 20 three-year rolling average data points. County-level characteristics from census data and from the Area Health Resources File were normalized and entered into a principal component analysis. Multinomial logistic regression models were used to test the relation between these factors (clusters of related contextual variables) and the disparity trend pattern group for each county. Counties were grouped into 4 disparity trend pattern groups: 1) persistent disparity (parallel black and white trend lines); 2) diverging (widening disparity); 3) sustained equality; and 4) converging (moving from disparate outcomes toward equality). The initial principal component analysis clustered the 82 independent variables into a smaller number of components, 6 of which explained 47% of the county-level variation in disparity trend patterns. County-level variation in social determinants, health care workforce, and health systems all were found to contribute to variations in cancer mortality disparity trend patterns from 1990 through 2010. Counties sustaining equality over time or moving from disparities to equality in cancer mortality suggest that disparities are not inevitable, and provide hope that more communities can achieve optimal and equitable cancer outcomes for all. Cancer 2016;122:1735-48. © 2016 American Cancer Society. © 2016 American Cancer Society.

Surface-water availability, Tuscaloosa County, Alabama

USGS Publications Warehouse

Knight, Alfred L.; Davis, Marvin E.

1975-01-01

The average annual runoff, about 1,270 mgd (million gallons per day), originating in Tuscaloosa County is equivalent to 20 inches or 0.95 mgd per square mile. The Black Warrior and Sipsey Rivers, the largest streams in the county, have average flows of 5,230 mgd and 580 mgd, respectively, where they leave the county, and median annual 7-day low flows in excess of 150 mgd and 35 mgd, respectively. North River, Big Sandy Creek, and Hurricane Creek have average flows in excess of 100 mgd and median annual 7-day low flows in excess of 2 mgd. Surface water generally contains less than 100 mg/l (milligrams per liter) dissolved solids, less than 10 mg/l chloride, and is soft to moderately hard. Streams having the higher hardness and the higher dissolved-solids content are in eastern Tuscaloosa County.

Consideration of driver home county prohibition and alcohol-related vehicle crashes.

PubMed

Schulte Gary, Sarah Lynn; Aultman-Hall, Lisa; McCourt, Matt; Stamatiadis, Nick

2003-09-01

This study examines the characteristics of alcohol-related crashes in wet versus dry counties in the state of Kentucky, USA and incorporates the location of driver residences through use of geographic information system (GIS) analysis. Between 1991 and 1997, 39344 alcohol-related crashes by Kentucky residents on Kentucky State roads were reported. The location of the crash and the home ZIP code from the driver's address were used to consider distance from home in the GIS. Analysis of the crash data revealed that a similar proportion of crashes in wet and dry counties are alcohol-related but that a higher proportion of dry counties residents are involved in an alcohol-related crash. However, when the distance from home variable is considered, several results suggest that dry county residents may be driving further when consuming alcohol. In part due to the rural nature of dry counties, drivers from dry counties have both alcohol-related and non-alcohol related crashes farther from their homes than residents from wet counties. Alcohol-related crashes by dry county residents in wet counties are the greatest average distance from home while crashes by wet county residents in wet counties are the smallest average distance. Drivers from dry counties over 21 years of age have alcohol-related crashes significantly farther from home than those under 21 who would not legally be admitted to drinking establishments in the wet counties. Furthermore, residents from dry counties that do not border wet counties have alcohol-related crashes on average farther from home than the border county residents. These last three results provide circumstantial evidence that some dry county drivers may be driving to wet counties to consume alcohol thus increasing impaired driving exposure. In conclusion, by considering crash location and driver residence, these findings indicate that county-level prohibition is not necessarily effective in improving highway safety.

75 FR 50987 - Madera County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-18

... DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Madera County Resource Advisory Committee will be... expenditure of Payments to States Madera County Title II funds. DATES: The meetings will be held on August...

76 FR 66033 - Madera County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-25

... DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Madera County Resource Advisory Committee will be... of 2000 (Pub. L.110-343) for expenditure of Payments to States Madera County Title II funds. DATES...

76 FR 7531 - Lake County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-10

... DEPARTMENT OF AGRICULTURE Forest Service Lake County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Lake County Resource Advisory Committee (RAC) will... meeting will be held at the Lake County Board of Supervisor's Chambers at 255 North Forbes Street...

75 FR 17897 - Lake County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-08

... DEPARTMENT OF AGRICULTURE Forest Service Lake County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Lake County Resource Advisory Committee (RAC) will... meeting will be held at the Lake County Board of Supervisor's Chambers at 255 North Forbes Street...

77 FR 57556 - Lake County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-18

... DEPARTMENT OF AGRICULTURE Forest Service Lake County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of Meeting. SUMMARY: The Lake County Resource Advisory Committee (RAC) will...: The meeting will be held at the Lake County Board of Supervisor's Chambers at 255 North Forbes Street...

Effects of short-term tocopherol (T) feeding on structure-localized protein tyrosine nitration (pTN) patterns of mitochondrial ATPase following endotoxin (LPS) challenge in beef calves.

USDA-ARS?s Scientific Manuscript database

Mitochondrial ATPase/Complex-V (MCV) is an electron transport chain (ETC) component needed for ATP synthesis. The ETC, exquisitely sensitive to proinflammatory mediators (PIM), generates oxynitrogen reactants leading to pTN formation as mitochondrial membrane leakage occurs. Immunohistochemical loca...

Problems Impacting Extension Program Quality at the County Level: Results from an Analysis of County Program Reviews Conducted in Florida

ERIC Educational Resources Information Center

Harder, Amy; Moore, Austen; Mazurkewicz, Melissa; Benge, Matt

2013-01-01

Needs assessments are an important tool for informing organizational development efforts in Extension. The purpose of the study reported here was to identify problems faced by county units within UF/IFAS Extension during county program reviews. The findings were drawn from the reports created after five county units experienced program reviews in…

A Cultural Resources Sample Survey in the Harlan County Lake Project Lands West of U.S. Highway 183 Harlan County, Nebraska

DTIC Science & Technology

1988-01-01

p ri r rnpy Harlan County LakeNebraska US Army Corps of Engineers Kansas City District American Resources Group, Ltd. 0’ Carbondale, Illinois A...Cultural Resources Sample Survey in the Harlan County Lake Project Lands West of U.S. Highway 183 Harlan County , Nebraska DTIC IELECTE ’-.... Author -D M...TI TLE (ad Subtitle) S. TYPE OF REPORT & PERIOD COVERED Final Report A Cultural Resources Sample Survey in the Harlan 1983-1984 County Lake Project

76 FR 6116 - Madera County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-03

... DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Madera County Resource Advisory Committee will be...-Determination Act of 2000 (Pub. L. 110-343) for expenditure of Payments to States Madera County Title II funds...

7. View showing placement of timber deck placement on chord ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. View showing placement of timber deck placement on chord and built up construction of top chord and continuous construction through top panel points, eye bar construction on bottom chord - Bridge No. 2.4, Spanning Boiling Fork Creek at Railroad Milepost JC-2.4, Decherd, Franklin County, TN

14. VARIOUS OUTBUILDINGS: a) OCTAGONAL STRUCTURE (center): WASH HOUSE b) ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. VARIOUS OUTBUILDINGS: a) OCTAGONAL STRUCTURE (center): WASH HOUSE b) SQUARE BUILDING WITH HIPPED ROOF (right front): SMOKEHOUSE c) BRICK BUILDING WITH END CHIMNEYS (left front): KITCHEN AND COOK'S BUILDING d) LONG BRICK BUILDING (in background): SERVANTS' QUARTERS (?) - Colonel McNeal House, Union & Bills Streets, Bolivar, Hardeman County, TN

Progress in reducing premature deaths in Wisconsin counties, 2000-2010.

PubMed

Nonnweiler, Thomas; Pollock, Elizabeth A; Rudolph, Barbara; Remington, Patrick L

2013-10-01

Measuring trends in a county's premature death rate is a straightforward method that can be used to assess a county's progress in improving the health of the population. Age-adjusted premature death rate data from Wisconsin Interactive Statistics on Health for persons less than 75 years of age were collected for the years 2000-2010. Overall 10-year percent change was calculated, compared, and ranked for all Wisconsin counties during this time period. Progress was assessed as excellent (25.0% or greater decline), very good (20.0%-24.9% decline), good (10.0%-19.9% decline), fair (0.0%-9.9% decline), or poor (any increase). Overall, premature death rates in counties declined by 16.8% over the 10-year period 2000-2010 in Wisconsin. Trends varied by county, with 8, 15, 37, 9, and 3 counties having excellent, very good, good, fair, and poor progress, respectively. The most improvement was seen in Kewaunee County (decreasing 38.3%) and the least progress in Lafayette County (increasing 4.8%). Trends in premature death rates were not related to the county's initial death rate, population, rurality, or income. Although premature death rates declined overall in Wisconsin during the 2000s, this progress varied across counties and was not related to baseline mortality rates or other county characteristics.

Hurricane exposure and county fetal death rates, utilization of a county environmental quality index for confounding control.

EPA Science Inventory

The effects of natural disasters on public health are a rising concern, with increasing severity of disaster events. Many disaster studies utilize county-level analysis, however most do not control for county level environmental factors. Hurricane exposure during pregnancy could ...

Water resources of King County, Washington

USGS Publications Warehouse

Richardson, Donald; Bingham, J.W.; Madison, R.J.; Williams, R.

1968-01-01

Although the total supply of water in King County is large, water problems are inevitable because of the large and rapidly expanding population. The county contains a third of the 3 million people in Washington, most of the population being concentrated in the Seattle metropolitan area. King County includes parts of two major physiographic features: the western area is part of the Puget Sound Lowland, and the eastern area is part of the Cascade Range. In these two areas, the terrain, weather, and natural resources (including water) contrast markedly. Average annual precipitation in the county is about 80 inches, ranging from about 30 inches near Puget Sound to more than 150 inches in parts of the Cascades. Annual evapotranspiration is estimated to range from 15 to 24 inches. Average annual runoff ranges from about 15 inches in the lowlands to more than 100 inches in the mountains. Most of the streamflow is in the major basins of the county--the Green-Duwamish, Lake Washington, and Snoqualmie basins. The largest of these is the Snoqualmie River basin (693 square miles), where average annual runoff during the period 1931-60 was about 79 inches. During the same period, annual runoff in the Lake Washington basin ( 607 square miles) averaged about 32 inches, and in the Green-Duwamish River basin (483 square miles), about 46 inches. Seasonal runoff is generally characterized by several high-flow periods in the winter, medium flows in the spring, and sustained low flows in the summer and fall. When floods occur in the county they come almost exclusively between October and March. The threat of flood damage is greatest on the flood plaits of the larger rivers, but in the Green-Duwamish Valley the threat was greatly reduced with the completion of Howard A. Hanson Dam in 1962. In the Snoqualmie River basin, where no such dam exists, the potential damage from a major flood increases each year as additional land is developed in the Snoqualmie Valley. 0nly moderate amounts of

Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: the follow-up study of gastrointestinal cancer patients.

PubMed

Smorodin, Eugeniy-P; Kurtenkov, Oleg-A; Sergeyev, Boris-L; Kodar, Kristel-E; Chuzmarov, Valentin-I; Afanasyev, Vladimir-P

2008-07-21

To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic alphaGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages I-IV without distant metastases were subjected to follow-up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and alphaGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut-off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (c2 = 3.89, all patients; c2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages III-IV (c2 = 4.71, gastric cancer; c2 = 4

Edentulism in high poverty rural counties.

PubMed

Mitchell, Jordan; Bennett, Kevin; Brock-Martin, Amy

2013-01-01

To examine the differences in oral health status among residents of high-poverty counties, as compared to residents of other rural or urban counties, specifically on the prevalence of edentulism. We used the 2005 Behavioral Risk Factor Surveillance System (BRFSS) and the 2006 Area Resource File (ARF). All analyses were conducted with SAS and SAS-callable SUDAAN, in order to account for weighting and the complex sample design. Characteristics significantly related to edentulism include: geographic location, gender, race, age, health status, employment, insurance, not having a usual source of care, education, marital status, presence of chronic disease, having an English interview, not deferring care due to cost, income, and dentist saturation within the county. Significant associations between high-poverty rural and other rural counties and edentulism were found, and other socioeconomic and health status indicators remain strong predictors of edentulism. © 2012 National Rural Health Association.

7 CFR 714.43 - Recommendation by county committee.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 7 2011-01-01 2011-01-01 false Recommendation by county committee. 714.43 Section 714... PENALTIES ERRONEOUSLY, ILLEGALLY, OR WRONGFULLY COLLECTED § 714.43 Recommendation by county committee.... After the recommendation of approval or disapproval is made by the county committee, the claim shall be...

7 CFR 714.43 - Recommendation by county committee.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 7 2010-01-01 2010-01-01 false Recommendation by county committee. 714.43 Section 714... PENALTIES ERRONEOUSLY, ILLEGALLY, OR WRONGFULLY COLLECTED § 714.43 Recommendation by county committee.... After the recommendation of approval or disapproval is made by the county committee, the claim shall be...

76 FR 12316 - Madera County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-07

... DEPARTMENT OF AGRICULTURE Forest Service Madera County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Madera County Resource Advisory Committee will be... Madera County Title II funds. DATES: The meetings will be held on March 9th, and March 16th, 2011 from 6...

7 CFR 7.10 - Conduct of county convention.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Conduct of county convention. 7.10 Section 7.10 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION AND... other purpose. (e) The county committee shall give advance public notice of the county convention which...

7 CFR 1230.634 - FSA county office report.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... report. The FSA county office will notify the FSA State office of the results of the referendum. Each FSA county office will transmit the results of the referendum in its county to the FSA State office. Such...

School Progress Report 2012. Montgomery County Public Schools

ERIC Educational Resources Information Center

Montgomery County Public Schools, 2013

2013-01-01

The 2012 School Progress Report for Montgomery County Public Schools (MCPS) provides state, county, and individual school performance data, as well as information on student attendance, high school graduation rates, and the professional qualifications of teachers at the state, district, and school levels. Montgomery County primary schools are…

75 FR 24944 - Adequacy Status of the Milwaukee-Racine, Door County, Manitowoc County, and Sheboygan County...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-06

... a State Implementation Plan (SIP) means that transportation activities will not produce new air... Ozone Redesignation and Maintenance Plans for Transportation Conformity Purposes AGENCY: Environmental... Sheboygan County, Wisconsin ozone nonattainment areas are adequate for use in transportation conformity...

A Profile of Hardee County, Florida.

ERIC Educational Resources Information Center

Beaulieu, Lionel J.; Anderson, Deborah S.

In 1977 leaders of Hardee County, Florida, listed relationships and attitudes of residents, rural atmosphere, environmental conditions, and economic potential among the county's strong points, and public service and facility improvements, developing economic potential, recreational and entertainment development, and planning and zoning as its most…

76 FR 4254 - Irish Potatoes Grown in Certain Designated Counties in Idaho, and Malheur County, Oregon...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-25

... Service 7 CFR Part 945 [Doc. No. AMS-FV-10-0109; FV11-945-1] Irish Potatoes Grown in Certain Designated... among eligible producers of Irish potatoes in certain designated counties in Idaho, and Malheur County... Irish potatoes grown in the production area. DATES: The referendum will be conducted from March 5 to...

Ecological subregion codes by county, coterminous United States

Treesearch

Victor A. Rudis

1999-01-01

This publication presents the National Hierarchical Framework of Ecological Units (ECOMAP 1993) by county for the coterminous United States. Assignment of the framework to individual counties is based on the predominant area by province and section to facilitate integration of county-referenced information with areas of uniform ecological potential. Included are maps...

The Lower Sevier River Basin Crop Monitor and Forecast Decision Support System: Exploiting Landsat Imagery to Provide Continuous Information to Farmers and Water Managers

NASA Astrophysics Data System (ADS)

Torres-Rua, A. F.; Walker, W. R.; McKee, M.

2013-12-01

The last century has seen a large number of innovations in agriculture such as better policies for water control and management, upgraded water conveyance, irrigation, distribution, and monitoring systems, and better weather forecasting products. In spite of this, irrigation management and irrigation water deliveries by farmers/water managers is still based on factors like water share amounts, tradition, and past experience on irrigation. These factors are not necessarily related to the actual crop water use; they are followed because of the absence of related information provided in a timely manner at an affordable cost. Thus, it is necessary to develop means to deliver continuous and personalized information about crop water requirements to water users/managers at the field and irrigation system levels so managers at these levels can better quantify the required versus available water for irrigation during the irrigation season. This study presents a new decision support system (DSS) platform that addresses the absence of information on actual crop water requirements and crop performance by providing continuous updated farm-based crop water use along with other farm performance indicators such as crop yield and farm management to irrigators and water managers. This DSS exploits the periodicity of the Landsat Satellite Mission (8 to 16 days, depending on the period of interest) to provide remote monitoring at the individual field and irrigation system levels. The Landsat satellite images are converted into information about crop water use, yield performance and field management through application of state-of-the-art semi-physical and statistical algorithms that provide this information at a pixel basis that are ultimately aggregated to field and irrigation system levels. A version of the DSS has been implemented for the agricultural lands in the Lower Sevier River, Utah, and has been operational since the beginning of the 2013 irrigation season. The main goal of

Educational and Demographic Profile: Madera County

ERIC Educational Resources Information Center

California Postsecondary Education Commission, 2004

2004-01-01

This profile uniquely presents a variety of educational and socioeconomic information for Madera County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

Educational and Demographic Profile: Sonoma County.

ERIC Educational Resources Information Center

California Postsecondary Education Commission, 2004

2004-01-01

This profile uniquely presents a variety of educational and socioeconomic information for Sonoma County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. The information provides a framework for enhanced communication and…

Educational and Demographic Profile: Humboldt County.

ERIC Educational Resources Information Center

California Postsecondary Education Commission, 2004

2004-01-01

This profile uniquely presents a variety of educational and socioeconomic information for Humboldt County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

Educational and Demographic Profile: Mariposa County.

ERIC Educational Resources Information Center

California Postsecondary Education Commission, 2004

2004-01-01

This profile uniquely presents a variety of educational and socioeconomic information for Mariposa County, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for enhanced…

Status Offender Project: County Data Reports--Buncombe County.

ERIC Educational Resources Information Center

Deimel, Betty; And Others

The first of three reports on a North Carolina project to help counties plan community based status offender programs describes a statewide needs assessment to collect information about the needs of status offenders and youth at risk of entering the juvenile justice system. A questionnaire covered situational and behavioral problems experienced…

Ground water in Creek County, Oklahoma

USGS Publications Warehouse

Cady, Richard Carlysle

1937-01-01

Creek County has been designated as a problem area by the Land Use Planning Section of the Resettlement Administration. Some of the earliest oil fields to brought into production were situated in and near this county, and new fields have been opened from time to time during the ensuing years. The production of the newer fields, however, has not kept pace with the exhaustion of the older fields, and the county now presents an excellent picture of the problems involved in adjusting a population to lands that are nearly depleted of their mineral wealth. Values of land have been greatly depressed; tax collection is far in arrears; tenancy is widespread; and in addition more people will apparently be forced to depend on the income from agriculture than the land seems capable of supporting. The county as a whole is at best indifferently suitable for general farming. The Land Use planning Section proposes to study the present and seemingly immanent maladjustments of population to the resources of the land, and make recommendations for their correction. The writer was detailed to the Land Use Planning Section of Region VIII for the purposes of making studies of ground water problems in the region. In Creek County two investigations were made. In September, 1936, the writer spent about ten days investigating the availability of ground water for the irrigation of garden crops during drouths. If it proved feasible to do this generally throughout the county, the Land Use Planning Section might be able to encourage this practice. The second investigation made by the writer was in regard to the extent to which ground water supplies have been damaged by oil well brines. He was in county for four days late in January 1937, and again in March, 1937. During part of the second field trip he was accompanied by R.M. Dixon, sanitary engineer of the Water Utilization Unit of the Resettlement Administration. (available as photostat copy only)

75 FR 63436 - Nevada and Placer Counties Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-15

... DEPARTMENT OF AGRICULTURE Forest Service Nevada and Placer Counties Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Nevada and Placer Counties Resource... Counties. ADDRESSES: The meeting will be held at the Placer County Water Agency office, 144 Ferguson, Rd...

Are older adults living in more equal counties healthier than older adults living in more unequal counties? A propensity score matching approach.

PubMed

Choi, HwaJung; Burgard, Sarah; Elo, Irma T; Heisler, Michele

2015-09-01

We assessed the potential contextual effect of income inequality on health by: 1) comparing individuals with similar socioeconomic status (SES) but who reside in counties with different levels of income inequality; and 2) examining whether the potential effect of county-level income inequality on health varies across SES groups. We used the Health and Retirement Study, a nationally representative study of Americans over the age of 50. Using propensity score matching, we selected SES-comparable individuals living in high-income inequality counties and in low-income inequality counties. We examined differences in self-rated overall health outcomes and in other specific physical/mental health outcomes between the two groups using logistic regression (n = 34,994) and imposing different sample restrictions based on residential duration in the area. We then used logistic regression with interactions to assess whether, and if so how, health outcomes differed among participants of different SES groups defined by wealth, income, and education. In bivariate analyses of the unmatched full sample, adults living in high-income inequality counties have worse health outcomes for most health measures. After propensity score matching, adults in high-income inequality counties had worse self-rated health status (AOR = 1.12; 95% CI 1.04-1.19) and were more likely to report diagnosed psychiatric problems (AOR = 1.08; 95% CI 0.99-1.19) than their matched counterparts in low-income inequality counties. These associations were stronger with longer-term residents in the area. Adverse health outcomes associated with living in high-income inequality counties were significant particularly for individuals in the 30(th) or greater percentiles of income/wealth distribution and those without a college education. In summary, after using more precise matching methods to compare individuals with similar characteristics and addressing measurement error by excluding more recently arrived county

Are older adults living in more equal counties healthier than older adults living in more unequal counties? A propensity score matching approach

PubMed Central

Choi, HwaJung; Burgard, Sarah; Elo, Irma T.; Heisler, Michele

2015-01-01

We assessed the potential contextual effect of income inequality on health by: 1) comparing individuals with similar socioeconomic status (SES) but who reside in counties with different levels of income inequality; and 2) examining whether the potential effect of county-level income inequality on health varies across SES groups. We used the Health and Retirement Study, a nationally representative study of Americans over the age of 50. Using propensity score matching, we selected SES-comparable individuals living in high-income inequality counties and in low-income inequality counties. We examined differences in self-rated overall health outcomes and in other specific physical/mental health outcomes between the two groups using logistic regression (n=34,994) and imposing different sample restrictions based on residential duration in the area. We then used logistic regression with interactions to assess whether, and if so how, health outcomes differed among participants of different SES groups defined by wealth, income, and education. In bivariate analyses of the unmatched full sample, adults living in high-income inequality counties have worse health outcomes for most health measures. After propensity score matching, adults in high-income inequality counties had worse self-rated health status (AOR=1.12; 95% CI 1.04–1.19) and were more likely to report diagnosed psychiatric problems (AOR=1.08; 95% CI 0.99–1.19) than their matched counterparts in low-income inequality counties. These associations were stronger with longer-term residents in the area. Adverse health outcomes associated with living in high-income inequality counties were significant particularly for individuals in the 30th or greater percentiles of income/wealth distribution and those without a college education. In summary, after using more precise matching methods to compare individuals with similar characteristics and addressing measurement error by excluding more recently arrived county residents

47. Photograph of a line drawing. '304 S.S. NUTSCHE BOX ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

47. Photograph of a line drawing. '304 S.S. NUTSCHE BOX AND FILTER PROBES, BUILDING H.' Holston Defense Corporation. June 24, 1955; revised 1958, 1960, 1965, 1968, 1982. Delineator: G. Sahlin. Drawing # 7651-1008/262. - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

18. DETAIL AT JUNCTION OF MAIN DAM AT LEFT AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

18. DETAIL AT JUNCTION OF MAIN DAM AT LEFT AND DIVERSION DAM AT RIGHT SHOWING LOG CRIBBING. SPACES INSIDE CRIBBING WERE FILLED WITH STONE TO ANCHOR DAM; DETERIORATION OF DAM HAS ALLOWED STONE BALLAST TO WASH AWAY. Photographed July 18, 1938. - Forge Creek Dam-John Cable Mill, Townsend, Blount County, TN

Educational and Demographic Profile: Kern County.

ERIC Educational Resources Information Center

California Postsecondary Education Commission, 2004

2004-01-01

This profile derived from U.S. Census 2000 data presents educational and socioeconomic information for Kern County, California, nearby counties, and the state. The profile highlights the relationship between various factors that affect the economic well-being of individuals and communities. This presentation of information provides a framework for…

TnBP⁄Triton X-45 Treatment of Plasma for Transfusion Efficiently Inactivates Hepatitis C Virus

PubMed Central

Chou, Ming-Li; Burnouf, Thierry; Chang, Shun-Pang; Hung, Ting-Chun; Lin, Chun-Ching; Richardson, Christopher D.; Lin, Liang-Tzung

2015-01-01

Risk of transmission of hepatitis C virus (HCV) by clinical plasma remains high in countries with a high prevalence of hepatitis C, justifying the implementation of viral inactivation treatments. In this study, we assessed the extent of inactivation of HCV during minipool solvent/detergent (SD; 1% TnBP / 1% Triton X-45) treatment of human plasma. Luciferase-tagged infectious cell culture-derived HCV (HCVcc) particles were used to spike human plasma prior to treatment by SD at 31 ± 0.5°C for 30 min. Samples were taken before and after SD treatment and filtered on a Sep-Pak Plus C18 cartridge to remove the SD agents. Risk of cytotoxicity was assessed by XTT cell viability assay. Viral infectivity was analyzed based on the luciferase signals, 50% tissue culture infectious dose viral titer, and immunofluorescence staining for HCV NS5A protein. Total protein, cholesterol, and triglyceride contents were determined before and after SD treatment and C18 cartridge filtration. Binding analysis, using patient-derived HCV clinical isolates, was also examined to validate the efficacy of the inactivation by SD. SD treatment effectively inactivated HCVcc within 30 min, as demonstrated by the baseline level of reporter signals, total loss of viral infectivity, and absence of viral protein NS5A. SD specifically targeted HCV particles to render them inactive, with essentially no effect on plasma protein content and hemostatic function. More importantly, the efficacy of the SD inactivation method was confirmed against various genotypes of patient-derived HCV clinical isolates and against HCVcc infection of primary human hepatocytes. Therefore, treatment by 1% TnBP / 1% Triton X-45 at 31°C is highly efficient to inactivate HCV in plasma for transfusion, showing its capacity to enhance the safety of therapeutic plasma products. We propose that the methodology used here to study HCV infectivity can be valuable in the validation of viral inactivation and removal processes of human

76 FR 9640 - Prevailing Rate Systems: Santa Clara, CA, Tulsa County, OK, and Angelina County, TX

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-22

... System (FWS) wage area and Angelina County, Texas, as an area of application to the Dallas, TX, NAF FWS... County, Texas, as an area of application to the Dallas, TX, NAF FWS wage area. The Federal Prevailing... area listing for the Oklahoma, OK, and Dallas, TX, NAF wage areas to read as follows: Appendix D to...

76 FR 41755 - Eastern Arizona Counties Resource Advisory; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-15

... DEPARTMENT OF AGRICULTURE Forest Service Eastern Arizona Counties Resource Advisory; Meeting AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Eastern Arizona Counties Resource... FURTHER INFORMATION CONTACT: Julia Faith Rivera, RAC Program Manager, Eastern Arizona Counties Resource...

Agreement between the Board of Trustees of the St. Clair County Community College of the County of St. Clair and the St. Clair County Community College District of the Michigan Association for Higher Education.

ERIC Educational Resources Information Center

Saint Clair County Community Coll., Port Huron, MI.

This agreement between the Board of Trustees of the St. Clair County Community College of the County of St. Clair and the St. Clair County Community College District of the Michigan Association for Higher Education covers the academic years 1972-74. Articles of the agreement cover recognition, association and instructor's rights, rights of the…

Mini epidemic of isoniazide resistant TB in rural TN: a need for supervised preventive therapy.

PubMed

Mehta, Jay; Keith, Rob; Al Hasan, Muhannad; Ryland, Byrd; Roy, Thomas

2009-08-01

With the resurgence of tuberculosis (TB) in the late 1980s, multi-drug-resistant TB (MDR-TB) also became a serious challenge to the TB control programs across the United States (US). While the incidence of TB resumed a downward trend in the mid 1900s, drug-resistant TB continues to be a national and international problem. We reviewed the public health data of drug-resistant TB cases (1996-2002) in Greene County, TN, with a detailed analysis of their contact investigation. Our study included demographic data of age, sex, race, human immunodeficiency virus (HIV) status and other known risk factors for drug-resistant TB. Contact investigation of two patients with isoniazide-resistant active pulmonary TB led to the discovery of two additional cases of active pulmonary tuberculosis, one of them being a 14-month-old child. All four of the patients were U.S. born, had negative HIV tests, and lacked other risk factors for drug-resistant TB. In all four cases, the Mycobacterium tuberculosis isolates were resistant to isoniazide, three were streptomycin resistant, and was ethambutol resistant. A total of 65 close contacts were identified, 11 of whom had a positive purified protein derivative (PPD) skin test indicating latent TB infection. Based on the American Thoracic Society's recommendations, the contacts with a positive PPD were prescribed rifampin for chemo-prevention rather than INH. However, one active case was detected from this infected contact who had failed to comply with chemo-preventive therapy. The second active case was a child who developed active pulmonary TB before chemoprevention could be initiated. Drug culture profile and DNA analysis (RFLP) confirmed the same source for TB transmission. The 11/65 (16.5 percent) infection rate among the contact was comparable to the state average (p < 0.05), but the case rate of 4/65 (6.15 percent) was high. In two out of four active cases, who were family members of the known cases, active infection could have been

Orange County Photovoltaic Project & Educational COmponent

DOE Office of Scientific and Technical Information (OSTI.GOV)

Parker, Renee

The purpose of this report is to discuss the projects implemented, utilizing Department of Energy grant funds, to support the use and understanding of renewable energy in Orange County, Florida and the Greater Orlando Area. Orange County is located in the State of Florida and is most popularly referred to as Orlando. The greater Orlando area’s current population is 1,225,267 and in 2015 was the first destination to surpass 60 million visitors. Orange County utilized grant funds to add to the growing demand for access to charging stations by installing one level 2 dual NovaCharge CT4021 electric vehicle charging stationmore » at the Orange County/University of Florida Cooperative Extension Center. The charging station is considered a “smart” charger connected to a central network operated by a third party. Data collected includes the number of charging sessions, session start and end times, the electricity usage, greenhouse gases saved and other pertinent data used for reporting purposes. Orange County continues to support the use of electric vehicles in Metro Orlando and this project continues to bring awareness to our public regarding using alternative vehicles. Additionally, we offer all visitors to the Orange County/University of Florida Cooperative Extension Center free charges for their electric vehicles 24 hours a day. Since the operation of the charging station there have been 52 unique driver users, a total of 532.2258 kg of greenhouse gas savings and 159.03 gallons of gasoline savings. The installation of the additional electric vehicle charging station is part of a county-wide goal of promoting implementation of renewable energy technologies as well as supporting the use of electric vehicles including the Drive Electric Orlando & Florida programs. http://driveelectricorlando.com/ & ; http://www.driveelectricflorida.org/ . Grant funds were also used for Outreach and Educational efforts. Educational efforts about renewable energy were accomplished

69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

69. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF LOWNDES COUNTY, 1931 ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C.L. Wood, the county engineer. Updated through the mid-1930s to show new federal aid-state roads. Compares modern system with older county system. Original scale: 1 in. to 1 mi. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

Source apportionment of nitrogen and phosphorus from non-point source pollution in Nansi Lake Basin, China.

PubMed

Zhang, Bao-Lei; Cui, Bo-Hao; Zhang, Shu-Min; Wu, Quan-Yuan; Yao, Lei

2018-05-03

Nitrogen (N) and phosphorus (P) from non-point source (NPS) pollution in Nansi Lake Basin greatly influenced the water quality of Nansi Lake, which is the determinant factor for the success of East Route of South-North Water Transfer Project in China. This research improved Johnes export coefficient model (ECM) by developing a method to determine the export coefficients of different land use types based on the hydrological and water quality data. Taking NPS total nitrogen (TN) and total phosphorus (TP) as the study objects, this study estimated the contributions of different pollution sources and analyzed their spatial distributions based on the improved ECM. The results underlined that the method for obtaining output coefficients of land use types using hydrology and water quality data is feasible and accurate, and is suitable for the study of NPS pollution at large-scale basins. The average output structure of NPS TN from land use, rural breeding and rural life is 33.6, 25.9, and 40.5%, and the NPS TP is 31.6, 43.7, and 24.7%, respectively. Especially, dry land was the main land use source for both NPS TN and TP pollution, with the contributed proportions of 81.3 and 81.8% respectively. The counties of Zaozhuang, Tengzhou, Caoxian, Yuncheng, and Shanxian had higher contribution rates and the counties of Dingtao, Juancheng, and Caoxian had the higher load intensities for both NPS TN and TP pollution. The results of this study allowed for an improvement in the understanding of the pollution source contribution and enabled researchers and planners to focus on the most important sources and regions of NPS pollution.

76 FR 28210 - Eastern Arizona Counties Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-16

... DEPARTMENT OF AGRICULTURE Forest Service Eastern Arizona Counties Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Eastern Arizona Counties will meet...: Julia Faith Rivera, RAC Program Manager, Eastern Arizona Counties Resource Advisory Committee, Apache...

Ground-water resources data for Baldwin County, Alabama

USGS Publications Warehouse

Robinson, James L.; Moreland, Richard S.; Clark, Amy E.

1996-01-01

Geologic and hydrologic data for 237 wells were collected, and water-levels in 223 wells in Baldwin and Escambia Counties were measured. Long-term water water-level data, available for many wells, indicate that ground-water levels in most of Baldwin County show no significant trends for the period of record. However, ground-water levels have declined in the general vicinity of Spanish Fort and Daphne, and ground-water levels in the Gulf Shores and Orange Beach areas are less than 5 feet above sea level in places. The quality of ground water generally is good, but problems with iron, sulfur, turbidity, and color occur. The water from most private wells in Baldwin County is used without treatment or filtration. Alabama public- health law requires that water from public-supply wells be chlorinated. Beyond that, the most common treatment of ground water by public-water suppliers in Baldwin County consists of pH adjustment, iron removal, and aeration. The transmissivity of the Miocene-Pliocene aquifer was determined at 10 locations in Baldwin County. Estimates of transmissivity ranged from 700 to 5,400 feet squared per day. In general, aquifer transmissivity was greatest in the southeastern part of the county, and least in the western part of the county near Mobile Bay. A storage coefficient of 1.5 x 10-3 was determined for the Miocene-Pliocene aquifer near Loxley.

Influence of Urbanicity and County Characteristics on the ...

EPA Pesticide Factsheets

Background: Air pollution epidemiology studies, often conducted in large metropolitan areas due to proximity to regulatory monitors, are limited in their ability to examine potential associations between air pollution exposures and health effects in rural locations. Methods: In a time-stratified case-crossover framework, we examined associations between asthma emergency department (ED) visits in North Carolina (2006-2008) collected by a surveillance system, and short-term ozone exposures using predicted concentrations from the Community Multiscale Air Quality (CMAQ) model. Associations were estimated by county groupings based on four urbanicity classifications (representative of county size and urban proximity) and county health. Results: Ozone was associated with asthma ED visits in all-year and warm season (April-October) analyses [Odds Ratio (OR) =1.019; 95% CI: 0.998, 1.040; OR=1.020; 95% CI: 0.997, 1.044, respectively, for a 20 ppb increase in lag 0-2 days ozone]. The association was strongest in Less Urbanized counties, with no evidence of a positive association in Rural counties. Associations were similar when adjusted for fine particulate matter in copolluant models. Associations were stronger for children (5-17 years of age) compared with other age groups, and for individuals living in counties with poorer health status compared with counties that had the highest health rankings, although estimated associations for these subgroups were imprecise. Conclu

75 FR 18145 - Eastern Arizona Counties Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-09

... DEPARTMENT OF AGRICULTURE Forest Service Eastern Arizona Counties Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Eastern Arizona Counties Resource... Rivera, Coordinator, Eastern Arizona Counties Resource Advisory Committee, c/o Forest Service, USDA, P.O...

The Farm Crisis and Decatur County.

ERIC Educational Resources Information Center

Flora, Jan L.; And Others

This case study assesses the impact of the farm sector on the economy and social organization of Decatur County (Kansas), a county which has historically depended on agriculture for its livelihood. Data were obtained from analysis of time series statistical indicators for the period between 1966 and 1984, questionnaire responses of local…

77 FR 49779 - Big Horn County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-17

... DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Big Horn County Resource Advisory Committee... Big Horn County Weed and Pest Building, 4782 Highway 310, Greybull, Wyoming. Written comments about...

75 FR 71069 - Big Horn County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-22

... DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Big Horn County Resource Advisory Committee... held at the Big Horn County Weed and Pest Building, 4782 Highway 310, Greybull, Wyoming. Written...

Geologic map of the Callville Bay Quadrangle, Clark County, Nevada, and Mohave County, Arizona

USGS Publications Warehouse

Anderson, R. Ernest

2003-01-01

Report: 139 Map Scale: 1:24,000 Map Type: colored geologic map A 1:24,000-scale, full-color geologic map and four cross sections of the Callville Bay 7-minute quadrangle in Clark County, Nevada and Mohave County, Arizona. An accompanying text describes 21 stratigraphic units of Paleozoic and Mesozoic sedimentary rocks and 40 units of Cenozoic sedimentary, volcanic, and intrusive rocks. It also discusses the structural setting, framework, and history of the quadrangle and presents a model for its tectonic development.

Geology and water resources of Winnebago County, Wisconsin

USGS Publications Warehouse

Olcott, Perry C.

1966-01-01

Sources or water in Winnebago County include surface water from the Fox and Wolf Rivers and their associated lakes, and ground water from sandstone, dolomite, and sand and gravel deposits. Surface water is hard and generally requires treatment, but is then suitable for municipal and most industrial uses. Pollution is only a local problem in the lakes and rivers, but algae are present in most of the lakes. Ground water in Winnebago County is hard to very hard, and dissolved iron is a problem in a large area of the county. A saline-water zone borders the eastern edge of the county and underlies the areas of concentrated pumpage at Neenah-Menasha and Oshkosh. A thick, southeastward-dipping sandstone aquifer, yielding as much as 1,000 gallons per minute to municipal and industrial wells, underlies Winnebago County. A dolomite aquifer in the eastern and southern part of the county yields as much as 50 gallons per minute to wells. Sand and gravel layers and lenses in preglacial bedrock channels, in northwestern Winnebago County and in the upper Fox River valley, yield as much as 50 gallons per minute to wells. Present water problems in the county include algae and local pollution in the Lake Winnebago Pool, iron in water from the sandstone aquifer, and saline ground Water in the eastern part of the county. Potential problems include rapid decline of water levels because of interference between closely spaced wells, migration of saline ground water toward areas of pumping, surface-water pollution from inadequate sewage and industrial-waste process plants, and ground-water pollution in dolomite formations. Development of the water resources of the county should follow a comprehensive plan which takes into consideration all aspects of water use. Dispersal of wells, especially extending toward the west from the heavily pumped Neenah-Menasha and Oshkosh areas, is recommended to reduce water-level declines and to avoid saline water. Supplemental use of ground water is

Cancer screening delivery in persistent poverty rural counties.

PubMed

Bennett, Kevin J; Pumkam, Chaiporn; Bellinger, Jessica D; Probst, Janice C

2011-10-01

Rural populations are diagnosed with cancer at different rate and stages than nonrural populations, and race/ethnicity as well as the area-level income exacerbates the differences. The purpose of this analysis was to explore cancer screening rates across persistent poverty rural counties, with emphasis on nonwhite populations. The 2008 Behavioral Risk Factor Surveillance System was used, combined with data from the Area Resource File (analytic n = 309 937 unweighted, 196 344 347 weighted). Unadjusted analysis estimated screening rates for breast, cervical, and colorectal cancer. Multivariate analysis estimated the odds of screening, controlling for individual and county-level effects. Rural residents, particularly those in persistent poverty counties, were less likely to be screened than urban residents. More African Americans in persistent poverty rural counties reported not having mammography screening (18.3%) compared to 15.9% of urban African Americans. Hispanics had low screening rates across all service types. Multivariate analysis continued to find disparities in screening rates, after controlling for individual and county-level factors. African Americans in persistent poverty rural counties were more likely to be screened for both breast cancer (odds ratio, 1.44; 95% confidence interval, 1.12-1.85) and cervical cancer (1.46; 1.07-1.99) when compared with urban whites. Disparities in cancer screening rates exist across not only race/ethnicity but also county type. These disparities cannot be fully explained by either individual or county-level effects. Programs have been successful in improving screening rates for African American women and should be expanded to target other vulnerable women as well as other services such as colorectal cancer screening.

Chester County ground-water atlas, Chester County, Pennsylvania

USGS Publications Warehouse

Ludlow, Russell A.; Loper, Connie A.

2004-01-01

Chester County encompasses 760 square miles in southeastern Pennsylvania. Groundwater-quality studies have been conducted in the county over several decades to address specific hydrologic issues. This report compiles and describes water-quality data collected during studies conducted mostly after 1990 and summarizes the data in a county-wide perspective.In this report, water-quality constituents are described in regard to what they are, why the constituents are important, and where constituent concentrations vary relative to geology or land use. Water-quality constituents are grouped into logical units to aid presentation: water-quality constituents measured in the field (pH, alkalinity, specific conductance, and dissolved oxygen), common ions, metals, radionuclides, bacteria, nutrients, pesticides, and volatile organic compounds. Water-quality constituents measured in the field, common ions (except chloride), metals, and radionuclides are discussed relative to geology. Bacteria, nutrients, pesticides, and volatile organic compounds are discussed relative to land use. If the U.S. Environmental Protection Agency (USEPA) or Chester County Health Department has drinking water standards for a constituent, the standards are included. Tables and maps are included to assist Chester County residents in understanding the water-quality constituents and their distribution in the county.Ground water in Chester County generally is of good quality and is mostly acidic except in the carbonate rocks and serpentinite, where it is neutral to strongly basic. Calcium carbonate and magnesium carbonate are major constituents of these rocks. Both compounds have high solubility, and, as such, both are major contributors to elevated pH, alkalinity, specific conductance, and the common ions. Elevated pH and alkalinity in carbonate rocks and serpentinite can indicate a potential for scaling in water heaters and household plumbing. Low pH and low alkalinity in the schist, quartzite, and

[Book review] The birds of San Diego County

USGS Publications Warehouse

Banks, R.C.

1985-01-01

San Diego County, California is larger in area than 2 of the 50 states (combined), its geographic (and thus biological) diversity ranges from the seacoast across mountains of nearly 2,000 m elevation to extreme desert, it is the southwesternmost county of the contiguous U.S., and it is inhabited by dedicated birders whose observations have boosted the county list to more than 450 species. "The Birds of San Diego County" provides detailed information on the geographic, ecological, and temporal distribution and abundance of each of the 449 species of native birds reported in the county-for some, by subspecies. Maps of breeding distribution are presented for 129 of the 181 breeding species (of which 26 are considered to be extirpated or only occasional breeders). Ecological zones and terms of abundance are defined precisely, and the definitions are adhered to. Welcomely absent are long para- graphs of descriptive and generalized information.

Assessment of Regeneration of Bone in the Extracted Third Molar Sockets Augmented Using Xenograft (CollaPlugTN Zimmer) in Comparison with the Normal Healing on the Contralateral Side.

PubMed

Ranganathan, Murugan; Balaji, M; Krishnaraj, R; Narayanan, Vivek; Thangavelu, Annamalai

2017-11-01

Alveolar bone resorption is a significant clinical problem. Bone loss in third molar region following extraction or surgical removal not only leads to periodontal problems in second molar region but also it may lead to some serious problems like increased incidence of angle fractures. In order to reduce the risks following third molar surgery, the socket should be augmented with bone grafts. In recent days guided tissue regeneration is the most accepted and successful technique followed many authors and its efficacy has been proved. Based upon our clinical experience, the use of bio absorbable collagen wound dressing such as CollaPlug TN has achieved quick healing and more primary wound coverage. Amongst the graft materials collagen is preferable due to its high biocompatibility and hemostatic ability. This study was done to assess the regeneration of bone in the extracted third molar sockets using xenograft (CollaPlug TN -Zimmer) which was compared with the normal healing on the contra lateral side. The assessment was done to analyze post-operative healing complications and to compare the bone density formed between control site and implant site radiologically. On this basis of this study, the use of collaplugTN appears to be beneficial to the patient in postoperative wound healing and also for better bone formation. The use of this material was advantageous because of its simplicity of application cost effectiveness and availability. There is enhanced wound healing and early bone formation.

50. Photograph of a line drawing. 'AREA B, GENERAL ASSEMBLY, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

50. Photograph of a line drawing. 'AREA B, GENERAL ASSEMBLY, BUILDINGS I, J, L, & M - LINES 9 & 10.' Holston Army Ammunition Plant. March 13, 1967; revised November 15, 1967, January 3, 1968. Delineator: Unknown. Drawing # 7651-1009.454. - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

75 FR 23820 - Notice of Docketing of Amendment Request for Materials License No. SNM-2506; Northern States...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-04

... INFORMATION CONTACT: Pamela Longmire, Ph.D., Project Manager, Licensing Branch, Division of Spent Fuel Storage... Generating Plant (PINGP), Unit Nos. 1 and 2, site in Goodhue County, Minnesota. The TN-40 cask is currently..., higher burnup spent fuel used in the PINGP reactor as well as associated changes to the ISFSI's technical...

60. View from stock bin trestle looking northeast at Babcock ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

60. View from stock bin trestle looking northeast at Babcock & Wilcox type boilers (manufactured by Casey-Hedges Co., Chattanooga, TN) where washed furnace gas is burned with natural gas and coal to generate heat for steam. - Sloss-Sheffield Steel & Iron, First Avenue North Viaduct at Thirty-second Street, Birmingham, Jefferson County, AL

Broward County Employers' Training Needs Assessment.

ERIC Educational Resources Information Center

Mehallis, Mantha Vlahos

An assessment of the training needs of Broward County, Florida, employers was conducted: (1) to determine the existing training needs of large businesses, industrial firms, and local units of government; (2) to determine which educational agencies in the county could fulfill these needs; and (3) to inform the companies of the results in order to…

Geology and ground-water resources of Rock County, Wisconsin

USGS Publications Warehouse

LeRoux, E.F.

1964-01-01

Rock County is in south-central Wisconsin adjacent to the Illinois State line. The county has an area of about 723 square miles and had a population of about 113,000 in 1957 ; it is one of the leading agricultural and industrial counties in the State. The total annual precipitation averages about 32 inches, and the mean annual temperature is about 48 ? F. Land-surface altitudes are generally between 800 and 00 feet, but range from 731 feet, where the Rock River flows into Illinois, to above 1,080 feet, at several places in the northwestern part of the county. The northern part of Rock County consists of the hills and kettles of a terminal moraine which slopes southward to a flat, undissected outwash plain. The southeastern part of the county is an area of gentle slopes, whereas the southwestern part consists of steep-sided valleys and ridges. Rock County is within the drainage basin of the Rock River, which flows southward through the center of the county. The western and southwestern parts of ,the county are drained by the Sugar River und Coon Creek, both of which flow into the Pecatonica River in Illinois and thence into the Rock River. The southeastern part of the county is drained by Turtle Creek, which also flows into Illinois before joining the Rock River. Nearly all the lakes and ponds are in the northern one-third of the county, the area of most recent glaciation. The aquifers in Rock County are of sedimentary origin and include deeply buried sandstones, shales, and dolomites of the Upper Cambrian series. This series overlies crystalline rocks of Precambrian age and supplies water to all the cities and villages in the county. The St. Peter sandstone of Ordovician age underlies all Rock County except where the formation has been removed by erosion in the Rock and Sugar River valleys, and perhaps in Coon Creek valley. The St. Peter sandstone is the principal source of water for domestic, stock, and small industrial wells in the western half of the county

76 FR 35997 - Onions Grown in Certain Designated Counties in Idaho, and Malheur County, OR; Modification of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-21

... Service 7 CFR Part 958 [Doc. No. AMS-FV-11-0025; FV11-958-1 PR] Onions Grown in Certain Designated... handling regulation for onions handled under the Idaho-Eastern Oregon onion marketing order. The marketing order regulates the handling of onions grown in designated counties in Idaho, and Malheur County, Oregon...

County Library Service to Rural Schools. Bulletin, 1930, No. 20

ERIC Educational Resources Information Center

Lathrop, Edith A.

1930-01-01

An efficient county library contemplates maximum service for money expended. Situated at the county seat or at some other centrally located place within the county, its book stock is carried to every resident of the county through a system branches, stations, and school deposits, and the services of an automobile and the parcel post. A trained…

Forest statistics for Chelan and Douglas Counties, Washington, 1959-60.

Treesearch

John W. Hazard

1963-01-01

This publication summarizes the results of the second forest inventory of Chelan and Douglas Counties, Washington. The collection of field data outside the National Forest was initiated in Chelan County in 1959, in Douglas County in 1960, and completed in both counties in 1960. National Forest resource data were collected during the period 1952 to 1958, and revised to...

Decomposing Mortality Disparities in Urban and Rural U.S. Counties.

PubMed

Spencer, Jennifer C; Wheeler, Stephanie B; Rotter, Jason S; Holmes, George M

2018-05-30

To understand the role of county characteristics in the growing divide between rural and urban mortality from 1980 to 2010. Age-adjusted mortality rates for all U.S. counties from 1980 to 2010 were obtained from the CDC Compressed Mortality File and combined with county characteristics from the U.S. Census Bureau, the Area Health Resources File, and the Inter-University Consortium for Political and Social research. We used Oaxaca-Blinder decomposition to assess the extent to which rural-urban mortality disparities are explained by observed county characteristics at each decade. Decomposition shows that, at each decade, differences in rural/urban characteristics are sufficient to explain differences in mortality. Furthermore, starting in 1990, rural counties have significantly lower predicted mortality than urban counties when given identical county characteristics. We find changes in the effect of characteristics on mortality, not the characteristics themselves, drive the growing mortality divide. Differences in economic and demographic characteristics between rural and urban counties largely explain the differences in age-adjusted mortality in any given year. Over time, the role these characteristics play in improving mortality has increased differentially for urban counties. As characteristics continue changing in importance as determinants of health, this divide may continue to widen. © Health Research and Educational Trust.

Hydrogeology and groundwater quality of Highlands County, Florida

USGS Publications Warehouse

Spechler, Rick M.

2010-01-01

Groundwater is the main source of water supply in Highlands County, Florida. As the demand for water in the county increases, additional information about local groundwater resources is needed to manage and develop the water supply effectively. To address the need for additional data, a study was conducted to evaluate the hydrogeology and groundwater quality of Highlands County. Total groundwater use in Highlands County has increased steadily since 1965. Total groundwater withdrawals increased from about 37 million gallons per day in 1965 to about 107 million gallons per day in 2005. Much of this increase in water use is related to agricultural activities, especially citrus cultivation, which increased more than 300 percent from 1965 to 2005. Highlands County is underlain by three principal hydrogeologic units. The uppermost water-bearing unit is the surficial aquifer, which is underlain by the intermediate aquifer system/intermediate confining unit. The lowermost hydrogeologic unit is the Floridan aquifer system, which consists of the Upper Floridan aquifer, as many as three middle confining units, and the Lower Floridan aquifer. The surficial aquifer consists primarily of fine-to-medium grained quartz sand with varying amounts of clay and silt. The aquifer system is unconfined and underlies the entire county. The thickness of the surficial aquifer is highly variable, ranging from less than 50 to more than 300 feet. Groundwater in the surficial aquifer is recharged primarily by precipitation, but also by septic tanks, irrigation from wells, seepage from lakes and streams, and the lateral groundwater inflow from adjacent areas. The intermediate aquifer system/intermediate confining unit acts as a confining layer (except where breached by sinkholes) that restricts the vertical movement of water between the surficial aquifer and the underlying Upper Floridan aquifer. The sediments have varying degrees of permeability and consist of permeable limestone, dolostone, or

Physicochemical and toxicological profiling of ash from the 2010 and 2011 eruptions of Eyjafjallajökull and Grímsvötn volcanoes, Iceland using a rapid respiratory hazard assessment protocol.

PubMed

Horwell, C J; Baxter, P J; Hillman, S E; Calkins, J A; Damby, D E; Delmelle, P; Donaldson, K; Dunster, C; Fubini, B; Kelly, F J; Le Blond, J S; Livi, K J T; Murphy, F; Nattrass, C; Sweeney, S; Tetley, T D; Thordarson, T; Tomatis, M

2013-11-01

The six week eruption of Eyjafjallajökull volcano in 2010 produced heavy ash fall in a sparsely populated area of southern and south eastern Iceland and disrupted European commercial flights for at least 6 days. We adopted a protocol for the rapid analysis of volcanic ash particles, for the purpose of informing respiratory health risk assessments. Ash collected from deposits underwent a multi-laboratory physicochemical and toxicological investigation of their mineralogical parameters associated with bio-reactivity, and selected in vitro toxicology assays related to pulmonary inflammatory responses. Ash from the eruption of Grímsvötn, Iceland, in 2011 was also studied. The results were benchmarked against ash from Soufrière Hills volcano, Montserrat, which has been extensively studied since the onset of eruptive activity in 1995. For Eyjafjallajökull, the grain size distributions were variable: 2-13 vol% of the bulk samples were <4 µm, with the most explosive phases of the eruption generating abundant respirable particulate matter. In contrast, the Grímsvötn ash was almost uniformly coarse (<3.5 vol%<4 µm material). Surface area ranged from 0.3 to 7.7 m2 g(-1) for Eyjafjallajökull but was very low for Grímsvötn (<0.6 m2 g(-1)). There were few fibre-like particles (which were unrelated to asbestos) and the crystalline silica content was negligible in both eruptions, whereas Soufrière Hills ash was cristobalite-rich with a known potential to cause silicosis. All samples displayed a low ability to deplete lung antioxidant defences, showed little haemolysis and low acute cytotoxicity in human alveolar type-1 like epithelial cells (TT1). However, cell-free tests showed substantial hydroxyl radical generation in the presence of hydrogen peroxide for Grímsvötn samples, as expected for basaltic, Fe-rich ash. Cellular mediators MCP-1, IL-6, and IL-8 showed chronic pro-inflammatory responses in Eyjafjallajökull, Grímsvötn and Soufrière Hills samples

76 FR 26240 - Big Horn County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-06

... words Big Horn County RAC in the subject line. Facsimilies may be sent to 307-674-2668. All comments... DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Big Horn County Resource Advisory Committee...

Forest area statistics for Midsouth counties

Treesearch

J. M. Earles

1973-01-01

This report summarizes acreage data on commercial forest land in counties of the seven States of the Midsouth. It includes some data that have been issued previously and some that have never been published at the county level. The information was gathered during 1963- 1972 by the Forest Resources Research Unit of the Southern Forest Experiment Station.

Forest statistics for middle Tennessee counties

Treesearch

J.M. Earles

1971-01-01

This report tabulates information from a new forest inventory of counties of central and west-central regions of Tennessee, completed in 1971 by the Southern Forest Experiment Station. The tables are intended for use as source data in compiling estimates for groups of counties. Because the sampling procedure is intended primarily to furnish inventory data for the State...

Characterization of Streptomycin Resistance in Isolates of Erwinia amylovora in California.

PubMed

Förster, Helga; McGhee, Gayle C; Sundin, George W; Adaskaveg, James E

2015-10-01

In surveys from 2006 to 2014, streptomycin resistance in Erwinia amylovora from pear-growing areas in California declined from very high incidence in 2006 and 2007 to very low incidence in 2013 and 2014. The majority of resistant strains were designated as moderately resistant-low (MR-L), and were almost exclusively found in Sacramento County, whereas highly resistant (HR) strains were only recovered in Sutter-Yuba and San Joaquin counties. Resistance of HR strains was associated with a mutation in codon 43 of the chromosomal rpsL gene that results in a change from lysine to arginine, the same mutation that was originally reported for resistant strains from California in the mid-1970s. MR-L strains were found to harbor the strA-strB streptomycin resistance genes on transposon Tn5393a. This transposon lacks insertion sequence IS1133 that provides a promoter for efficient expression of strA-strB, resulting in lower minimum inhibitory concentrations of MR-L strains compared with those from other locations that harbor strA-strB on Tn5393::IS1133. In contrast to previously described plasmid-mediated resistance where Tn5393 is inserted in pEa34, or pEA29, Tn5393a in MR-L strains was located on plasmid pEU30. This plasmid was first described in E. amylovora from the western United States but was not associated with streptomycin resistance determinants previously. We hypothesize that Tn5393a was introduced into an E. amylovora strain carrying pEU30 and transposed into that plasmid. This hypothesis was supported by clustered regularly interspaced short palindromic repeat (CRISPR) sequence analysis that showed that two MR-L strains share the same CRISPR1 pattern as a streptomycin-sensitive strain. With current low resistance levels in California growing regions, streptomycin could be successfully used again, but applications per season should be limited and the antibiotic should be mixed and rotated with different modes of action.

Ground-water resources of Cumberland County, New Jersey

USGS Publications Warehouse

Rooney, James G.

1971-01-01

Water use in Cumberland County varies and is highly seasonal, mainly because of increasing requirements for irrigation and the food processing industries in the county. In 1964 seasonal use ranged from 27 mgd in March to 145 mgd in August. This is much higher than withdrawals in neighboring Salem and Cape May Counties. In 1964 withdrawals in Cumberland County averaged about 51 mgd; almost all of this, 49.4 mgd, was from ground-water supplies. The total annual water use in 1964 according to type of use was: for public supply, 10.6 mgd; for industrial uses, 19.0 mgd; irrigation, 15.4 mgd; suburban, rural, residential, institutional, farm, and commercial, 5.9 mgd. 

78 FR 33326 - Big Horn County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-04

... DEPARTMENT OF AGRICULTURE Forest Service Big Horn County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Big Horn County Resource Advisory Committee... will be held July 15, 2013 at 3:00 p.m. ADDRESSES: The meeting will be held at Big Horn County Weed and...

Patterns of gun deaths across US counties 1999-2013.

PubMed

Kalesan, Bindu; Galea, Sandro

2017-05-01

We examined the socio-demographic distribution of gun deaths across 3143 counties in 50 United States' states to understand the spatial patterns and correlates of high and low gun deaths. We used aggregate counts of gun deaths and population in all counties from 1999 to 2013 from the Centers for Disease Control and Prevention's Wide-ranging Online Data for Epidemiologic Research (WONDER). We characterized four levels of gun violence, as distinct levels of gun death rates of relatively safe, unsafe, violent, and extremely violent counties, based on quartiles of 15-year county-specific gun death rates per 100,000 and used negative binomial regression models allowing clustering by state to calculate incidence rate ratios and 95% confidence intervals (95% CIs). Most states had at least one violent or extremely violent county. Extremely violent gun counties were mostly rural, poor, predominantly minority, had high unemployment rate and homicide rate. Overall, homicide rate was significantly associated with gun deaths (incidence rate ratios = 1.08, 95% CI = 1.06-1.09). In relatively safe counties, this risk was 1.09 (95% CI = 1.05-1.13) and in extremely violent gun counties was 1.03 (95% CI = 1.03-1.04). There are broad differences in gun death rates across the United States representing different levels of gun death rates in each state with distinct socio-demographic profiles. Copyright © 2017 Elsevier Inc. All rights reserved.

Costs of raccoon rabies incidents in cattle herds in Hampshire County, West Virginia, and Guernsey County, Ohio.

PubMed

Chipman, Richard B; Cozzens, Tyler W; Shwiff, Stephanie A; Biswas, Rita; Plumley, Jewell; O'Quin, Jeanette; Algeo, Timothy P; Rupprecht, Charles E; Slate, Dennis

2013-12-01

To determine direct and indirect costs associated with raccoon rabies incidents involving cattle herds in Hampshire County, WV, in 2008 and Guernsey County, Ohio, in 2010. Ex post cost analysis. 1 cattle herd in Hampshire County, WV, in 2008 and 1 cattle herd in Guernsey County, Ohio, in 2010. Data were collected for each incident through telephone and email interviews with 16 federal, state, and county agency personnel involved in the case investigations and coordinated responses for rabies in the cattle herds. To characterize the economic impact associated with rabies in the 2 cattle herds, cost analysis was conducted with 7 cost variables (salary and benefits for personnel involved in the response, human postexposure prophylaxis, indirect patient costs, rabies diagnostic testing, cattle carcass disposal, market value of euthanized cattle, and enhanced rabies surveillance). Estimates of direct costs were determined on the basis of agency records and other relevant data obtained from notes and reports made by agency staff at the time of the incident and from a review of the literature. Primary costs included the market value of euthanized cattle ($51,461 in West Virginia; $12,561 in Ohio), human postexposure prophylaxis ($17,959 in West Virginia; $11,297 in Ohio), and salary and benefits for personnel involved in the response ($19,792 in West Virginia; $14,496 in Ohio). These results should provide a basis for better characterization of the economic impact of wildlife rabies in cattle in the United States.

Ionothermal synthesis of discrete supertetrahedral Tn (n = 4, 5) clusters with tunable components, band gaps, and fluorescence properties.

PubMed

Yang, Dan-Dan; Li, Wei; Xiong, Wei-Wei; Li, Jian-Rong; Huang, Xiao-Ying

2018-05-01

The preparation of crystalline molecularly supertetrahedral Tn clusters with variable sizes and components is of vital importance for the fundamental study of their physicochemical properties. However, setting up an efficient method to stabilize large discrete Tn clusters is a challenge due to their high negative charges and polymerization nature. In this work, we report on the ionothermal synthesis of three discrete T4 cluster compounds, namely [Bmmim]5[(CH3)2NH2]4[NH4][M4In16S31(SH)4]·6H2O (M = Mn (1), Zn (2), Cd (3), Bmmim = 1-buty-2,3-dimethyl-imidazolium), and four discrete T5 cluster compounds, namely [Bmmim]10[NH4]3[Cu5Ga30-xInxS52(SH)4] (x = 6.6 (5), 14.5 (6), 23.8 (7), and 30 (8)). The compound [Bmmim]10[NH4]3[Cu5Ga30S52(SH)4] (4) previously reported by us features a discrete T5 cluster. The steep UV-Vis absorption edges indicate band gaps of 2.20 eV for 1, 2.64 eV for 2, 2.69 eV for 3, 3.04 eV for 4, 2.65 eV for 5, 2.48 eV for 6, 2.32 eV for 7, and 2.30 eV for 8. The compositions of T5 clusters could be varied with the ratios of Ga : In in the starting reagents, providing an opportunity to systematically control the band gaps and fluorescence performances of T5 cluster-based compounds. This research might advance the understanding of the ionothermal preparation and functionality tuning of crystalline chalcogenides.

33 CFR 100.905 - Door County Triathlon; Door County, WI.

Code of Federal Regulations, 2010 CFR

2010-07-01

... in position 45°00′46″ N, 087°20′30″ W. (DATUM: NAD 83). (b) Special Local Regulations. The...; Door County, WI. (a) Regulated Area. A regulated area is established to include all waters of Green Bay...

75 FR 51105 - Proposed Finding Against Federal Acknowledgment of the Central Band of Cherokee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-18

... functioned as a single autonomous political entity. The evidence clearly establishes that the petitioner does... that a woman who was born about 1895 in Lawrence County, TN, was ``a small woman under 5 feet, said to... evidence to corroborate any of their claims. There is no evidence that these men and women from divergent...

Annual Performance Evaluation of a Pair of Energy Efficient Houses (WC3 and WC4) in Oak Ridge, TN

DOE Office of Scientific and Technical Information (OSTI.GOV)

Biswas, Kaushik; Christian, Jeffrey E; Gehl, Anthony C

2012-04-01

Beginning in 2008, two pairs of energy-saver houses were built at Wolf Creek in Oak Ridge, TN. These houses were designed to maximize energy efficiency using new ultra-high-efficiency components emerging from ORNL s Cooperative Research and Development Agreement (CRADA) partners and others. The first two houses contained 3713 square feet of conditioned area and were designated as WC1 and WC2; the second pair consisted of 2721 square feet conditioned area with crawlspace foundation and they re called WC3 and WC4. This report is focused on the annual energy performance of WC3 and WC4, and how they compare against a previouslymore » benchmarked maximum energy efficient house of a similar footprint. WC3 and WC4 are both about 55-60% more efficient than traditional new construction. Each house showcases a different envelope system: WC3 is built with advanced framing featured cellulose insulation partially mixed with phase change materials (PCM); and WC4 house has cladding composed of an exterior insulation and finish system (EIFS). The previously benchmarked house was one of three built at the Campbell Creek subdivision in Knoxville, TN. This house (CC3) was designed as a transformation of a builder house (CC1) with the most advanced energy-efficiency features, including solar electricity and hot water, which market conditions are likely to permit within the 2012 2015 period. The builder house itself was representative of a standard, IECC 2006 code-certified, all-electric house built by the builder to sell around 2005 2008.« less

76 FR 13172 - Placer County Water Agency

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-10

... Water Agency Notice of Application Tendered for Filing with the Commission and Establishing Procedural... County Water Agency e. Name of Project: Middle Fork American River Project f. Location: The Middle Fork...) h. Applicant Contact: Andy Fecko, Project Manager, Placer County Water Agency, 144 Ferguson Road...

Ground-water resources of Audrain County, Missouri

USGS Publications Warehouse

Emmett, L.F.; Imes, J.L.

1984-01-01

The deep (principal) aquifer in Audrain County has an average thickness of about 1,300 feet and is composed of dolomite and minor quantities of sandstone of Cambrian and Ordovician age. The deep aquifer is the source of water for all public-supply and irrigation wells in Audrain County. Pumpage from the deep aquifer has caused a decrease in hydraulic head of more than 200 feet since 1900 in the vicinity of the city of Mexico. Calculations from a two-dimensional digital model of the deep aquifer indicate that the drawdown would increase 10 to 25 feet from May 1979 levels in Audrain County by May 2000 in the absence of irrigation pumpage and if public-supply wells continue to pump at the 1980 rate. If the additional stress due to seasonal irrigation is continued at 1980 pumping rates, 60 +/- 20 feet of drawdown is predicted by May 2000. Audrain County is the northernmost extent of freshwater in this aquifer in Missouri. In Audrain County the dissolved-solids concentration of water from this aquifer varies from 1,200 milligrams per liter in the north to less than 400 milligrams per liter in the south. Lowered water levels in the aquifer may allow water with a larger dissolved-solids concentration to move into the area. (USGS)

Brownfield to Brightfield Initiative in Oak Ridge, TN - 12346

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hough, Gil; Fairless, Chad

Experience characterizing, permitting, and restoring 'Brownfield' sites-government or industrial sites with restricted future use due to the presence or potential presence of hazardous substances, pollutants, or contaminants-is being leveraged to identify opportunities for redevelopment into solar power generating facilities which, in this context, are called 'Brightfields'. Brownfield sites offer the expansive land necessary for large photovoltaic (PV) solar farms, but require an in-depth working knowledge of complicated regulatory restrictions and environmental constraints to develop them. As a part of the effort to identify opportunities for redevelopment of Brownfield sites for solar applications, a technical guide, was composed specifically for themore » development of solar generation on restricted use sites. The basis of the technical guide gives specific consideration to environmental requirements and installation methods breaking that into three areas for assessing: 1) levels of contamination, 2) ground penetration requirements, and 3) the requirements for aesthetics and maintenance. Brightfield projects are underway to support the technical guide and expand re-industrialization efforts for the former DOE Gaseous Diffusion Plant in Oak Ridge, TN. There are exciting opportunities to turn Brownfields into Brightfield solar energy solutions for meeting the future renewable energy needs of our country. Brownfields that offer the large surface area required for solar PV farms coupled with the technical guide for the installation of solar farms on restricted use sites supports efforts to develop the solar capacities and expertise to tap this future market. The initial projects designed following the technical guide will provide verification of the installation requirements and beneficial reuse of restricted use sites. (authors)« less

Tourism impacts of Three Mile Island and other adverse events: Implications for Lincoln County and other rural counties bisected by radioactive wastes intended for Yucca Mountain

DOE Office of Scientific and Technical Information (OSTI.GOV)

Himmelberger, J.J.; Ogneva-Himmelberger, Y.A.; Baughman, M.

Whether the proposed Yucca Mountain nuclear waste repository system will adversely impact tourism in southern Nevada is an open question of particular importance to visitor-oriented rural counties bisected by planned waste transportation corridors (highway or rail). As part of one such county`s repository impact assessment program, tourism implications of Three Mile Island (TMI) and other major hazard events have been revisited to inform ongoing county-wide socioeconomic assessments and contingency planning efforts. This paper summarizes key research implications of such research as applied to Lincoln County, Nevada. Implications for other rural counties are discussed in light of the research findings. 29more » refs., 3 figs., 1 tab.« less

Vision for the Year 2002: A Working Document. Salem County Board for Vocational Education Strategic Plan for the Salem County Vocational Technical Schools.

ERIC Educational Resources Information Center

Salem County Board for Vocational Education, Woodstown, NJ.

A strategic plan for vocational-technical education (VTE) in Salem County, New Jersey, in the year 2002 was developed by the county board of VTE and county advisory board with input from students, parents, employers, elected officials, and the community in general. Six strategic planning subcommittees were formed to review the district's VTE…

Road infrastructure, spatial spillover and county economic growth

NASA Astrophysics Data System (ADS)

Hu, Zhenhua; Luo, Shuang

2017-09-01

This paper analyzes the spatial spillover effect of road infrastructure on the economic growth of poverty-stricken counties, based on the spatial Durbin model, by using the panel data of 37 poor counties in Hunan province from 2006 to 2015. The results showed that there is a significant spatial dependence of economic growth in Poor Counties. Road infrastructure has a positive impact on economic growth, and the results will be overestimated without considering spatial factors. Considering the spatial factors, the road infrastructure will promote the economic growth of the surrounding areas through the spillover effect, but the spillover effect is restricted by the distance factor. Capital investment is the biggest factor of economic growth in poor counties, followed by urbanization, labor force and regional openness.

Volcano Deformation and Eruption Forecasting using Data Assimilation: Case of Grimsvötn volcano in Iceland

NASA Astrophysics Data System (ADS)

Bato, Mary Grace; Pinel, Virginie; Yan, Yajing

2016-04-01

The recent advances in Interferometric Synthetic Aperture Radar (InSAR) imaging and the increasing number of continuous Global Positioning System (GPS) networks recorded on volcanoes provide continuous and spatially extensive evolution of surface displacements during inter-eruptive periods. For basaltic volcanoes, these measurements combined with simple dynamical models (Lengliné et al. 2008 [1], Pinel et al, 2010 [2], Reverso et al, 2014 [3]) can be exploited to characterise and constrain parameters of one or several magmatic reservoirs using inversion methods. On the other hand, data assimilation-a time-stepping process that best combines models and observations, sometimes a priori information based on error statistics to predict the state of a dynamical system-has gained popularity in various fields of geoscience (e.g. ocean-weather forecasting, geomagnetism and natural resources exploration). In this work, we aim to first test the applicability and benefit of data assimilation, in particular the Ensemble Kalman Filter [4], in the field of volcanology. We predict the temporal behaviors of the overpressures and deformations by applying the two-magma chamber model of Reverso et. al., 2014 [3] and by using synthetic deformation data in order to establish our forecasting strategy. GPS time-series data of the recent eruptions at Grimsvötn volcano is used for the real case applicability of the method. [1] Lengliné, O., D Marsan, J Got, V. Pinel, V. Ferrazzini, P. Obuko, Seismicity and deformation induced by magma accumulation at three basaltic volcanoes, J. Geophys. Res., 113, B12305, 2008. [2] V. Pinel, C. Jaupart and F. Albino, On the relationship between cycles of eruptive activity and volcanic edifice growth, J. Volc. Geotherm. Res, 194, 150-164, 2010 [3] T. Reverso, J. Vandemeulebrouck, F. Jouanne, V. Pinel, T. Villemin, E. Sturkell, A two-magma chamber as a source of deformation at Grimsvötn volcano, Iceland, JGR, 2014 [4] Evensen, G., The Ensemble Kalman

71. MISSISSIPPI, LOWNDES CO. COLUMBUSSTEENS MAP OF LOWNDES COUNTY, 1931 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

71. MISSISSIPPI, LOWNDES CO. COLUMBUS-STEENS MAP OF LOWNDES COUNTY, 1931 Detail of ROAD MAP OF LOWNDES COUNTY, MISSISSIPPI, 1931 by C. L. wood, county engineer. Orig. scale: I in. to I mi. Includes Columbus and area NE to steens. Extent: 12 mi. East-West, 9 mi. North-South. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS

75 FR 4417 - Wind Cave National Park, Custer County, SD

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-27

... DEPARTMENT OF THE INTERIOR Wind Cave National Park, Custer County, SD AGENCY: National Park... Final Environmental Impact Statement, Wind Cave National Park, Custer County, South Dakota. SUMMARY... Management Plan and Final Environmental Impact Statement (Plan), Wind Cave National Park, Custer County...

Groundwater environmental tracer data collected from the Chicot, Evangeline, and Jasper aquifers in Montgomery County and adjacent counties, Texas, 2008

USGS Publications Warehouse

Oden, Timothy D.

2011-01-01

The Gulf Coast aquifer system is the primary water supply for Montgomery County in southeastern Texas, including part of the Houston metropolitan area and the cities of Magnolia, Conroe, and The Woodlands Township, Texas. The U.S. Geological Survey, in cooperation with the Lone Star Groundwater Conservation District, collected environmental tracer data in the Gulf Coast aquifer system, primarily in Montgomery County. Forty existing groundwater wells screened in the Gulf Coast aquifer system were selected for sampling in Montgomery County (38 wells), Waller County (1 well), and Walker County (1 well). Groundwater-quality samples, physicochemical properties, and water-level data were collected once from each of the 40 wells during March-September 2008. Groundwater-quality samples were analyzed for dissolved gases and the environmental tracers sulfur hexafluoride, chlorofluorocarbons, tritium, helium-4, and helium-3/tritium. Water samples were collected and processed onsite using methods designed to minimize changes to the water-sample chemistry or contamination from the atmosphere. Replicate samples for quality assurance and quality control were collected with each environmental sample. Well-construction information and environmental tracer data for March-September 2008 are presented.

77 FR 16318 - Environmental Assessment: Jessamine County and Madison County, KY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-20

... County Connector while considering environmental, social, engineering, costs and other factors in the..., following a project kick-off coordinated through a media news release. An invitation letter will be sent to... proposed project, and further defining [[Page 16319

24. Duplicate negative of an historic negative. 'AERIAL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. Duplicate negative of an historic negative. 'AERIAL VIEW OF AREA 'B' HOLSTON ORDNANCE WORKS.' 1944. #OCMH 4-12.2ASAV3 in Super Explosives Program RDX and Its Composition A, B, & C, Record Group No. 319, National Archives, Washington, D.C. - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

51. Photograph of a line drawing. 'AREA B, ARRANGEMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

51. Photograph of a line drawing. 'AREA B, ARRANGEMENT OF KETTLES AND DRIVE PLAN, BUILDINGS I, J, L, & M - LINES 9 & 10.' Holston Army Ammunition Plant. March 14, 1967; revised November 14, 1967, January 3, 1968. Delineator: Unknown. Drawing # 7651-1009.456. - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

County-Level Population Economic Status and Medicare Imaging Resource Consumption.

PubMed

Rosenkrantz, Andrew B; Hughes, Danny R; Prabhakar, Anand M; Duszak, Richard

2017-06-01

The aim of this study was to assess relationships between county-level variation in Medicare beneficiary imaging resource consumption and measures of population economic status. The 2013 CMS Geographic Variation Public Use File was used to identify county-level per capita Medicare fee-for-service imaging utilization and nationally standardized costs to the Medicare program. The County Health Rankings public data set was used to identify county-level measures of population economic status. Regional variation was assessed, and multivariate regressions were performed. Imaging events per 1,000 Medicare beneficiaries varied 1.8-fold (range, 2,723-4,843) at the state level and 5.3-fold (range, 1,228-6,455) at the county level. Per capita nationally standardized imaging costs to Medicare varied 4.2-fold (range, $84-$353) at the state level and 14.1-fold (range, $33-$471) at the county level. Within individual states, county-level utilization varied on average 2.0-fold (range, 1.1- to 3.1-fold), and costs varied 2.8-fold (range, 1.1- to 6.4-fold). For both large urban populations and small rural states, Medicare imaging resource consumption was heterogeneously variable at the county level. Adjusting for county-level gender, ethnicity, rural status, and population density, countywide unemployment rates showed strong independent positive associations with Medicare imaging events (β = 26.96) and costs (β = 4.37), whereas uninsured rates showed strong independent positive associations with Medicare imaging costs (β = 2.68). Medicare imaging utilization and costs both vary far more at the county than at the state level. Unfavorable measures of county-level population economic status in the non-Medicare population are independently associated with greater Medicare imaging resource consumption. Future efforts to optimize Medicare imaging use should consider the influence of local indigenous socioeconomic factors outside the scope of traditional beneficiary-focused policy