Sample records for shita genshiryoku bunya
-
Sidwell, Robert W; Smee, Donald F
2003-01-01
When considering viruses of potential importance as tools for bioterrorism, several viruses in the Bunya- and Togaviridae families have been cited. Among those in the Bunyaviridae family are Rift Valley fever, Crimean-Congo hemorrhagic fever, hanta, and sandfly fever viruses, listed in order of priority. Those particularly considered in the Togaviridae family are Venezuelan, eastern and western equine encephalitis viruses. Factors affecting the selection of these viruses are the ability for them to induce a fatal or seriously incapacitating illness, their ease of cultivation in order to prepare large volumes, their relative infectivity in human patients, their ability to be transmitted by aerosol, and the lack of measures available for their control. Each factor is fully considered in this review. Vaccines for the control of infections induced by these viruses are in varying stages of development, with none universally accepted to date. Viruses in the Bunyaviridae family are generally sensitive to ribavirin, which has been recommended as an emergency therapy for infections by viruses in this family although has not yet been FDA-approved. Interferon and interferon inducers also significantly inhibit these virus infections in animal models. Against infections induced by viruses in the Togaviridae family, interferon-alpha would appear to currently be the most useful for therapy.
-
JPRS Report Science & Technology Japan
1989-10-25
Testing Slated for New BWR Fuel Assemblies [GENSHIRYOKU SANGYO SHIMBUN, 25 May 89] .... 37 Nuclear Fuel Planning System Developed [GENSHIRYOKU... Development (Debt) 13,272 ((Debt) 3,839) 7,995 (3,610) In addition, the budget has guaranteed that the following programs will proceed according... develop a combined cycle engine that will be capable of attaining high reliability and good fuel consumption at a wide range of speeds from low speed to
-
JPRS Report, Science & Technology, Japan.
1988-08-03
SHIMBUN, 4 Feb 88] 72 Advanced Reactor Design System To Be Developed [GENSHIRYOKU SANGYO SHIMBUN, 4 Feb 88] 73 Functional Testing on " Mutsu ...new types of reactors. 13008 74 NUCLEAR ENGINEERING FUNCTIONAL TESTING ON " MUTSU " SCHEDULED 43062060c Tokyo GENSHIRYOKU SANGYO SHIMBUN in Japanese...and to rebuild the power supply rectifiers used in the instrumentation controls on the nuclear powered ship, the " Mutsu ," which was launched on 27
-
The geological processes time scale of the Ingozersky block TTG complex (Kola Peninsula)
NASA Astrophysics Data System (ADS)
Nitkina, Elena
2013-04-01
complex rocks, the following age-formation stages are determined: protolith of the biotite gneisses - 3149±46 Ma; metamorphism, deformation of rocks, foliation - 2727±5 - 2725±2 - 2697±9 - 2667±7 Ma, granite bodies formation - 2615±8 Ma and biotite gneisses migmatization - 2549±30 Ma, formation of different pegmatite and granite veins -1644±7 Ma. Author are grateful to Akad. Mitrofanov F.P. and Bayanova T.B. for the consultations. The work is supported by RFBR 12-05-31063, 11-05-00570. 1.Batieva I.D., Belkov I.V. Granitoidnie formacii Kolskogo poluostrova. // Ocgerki po petrologiy, mineralogiy i metallogeniy Kolskogo poluostrova. L.: Nauka. 1968. p. 5-143. (in russian) 2. Belkov I.V., Zagorodny V.G., Predovsky A.A. et al. Stratigraficheskoe raschlenenie i korrelyacia dokembria severo-vostochoi chasty Baltiyskogo shita. L.: Nauka. 1971. p. 141-150. (in russian) 3. Docembriskaya tektonica severo-vostochoi chasty Baltiyskogo shita (Ob'asnitelnaya zapiska k tektonicheskoi karte severo-vostochoi chasty Baltiyskogo shita 1:500000) / ed.: F.P.Mitrofanov. Apatity: KFAN SSSR. 1992. 112 P. (in russian) 4. Zagorodny V.G., Radchenko A.T. Tectonika i glubinnoe stroenie severo-vostochoi chasty Baltiyskogo shita. Apatity: KFA SSSR. 1978. p. 3-12. (in russian) 5. Kozlov N.E., Sorohtin N.O., Glaznev V.N. et al. Geologia Arhea Baltiskogo shita. S.Pb.: Nauka. 2006. 329 p. (in russian) 6. Mitrofanov F.P. Sovremennie problemy i nekotorie resheniya dokembriskoy geologii kratonov. (2001) Litosphera.2001. V 1. P. 5-14. (in russian) 7. Ob'asnitelnaya zapiska k geologicheskoy karte severo-vostochoi chasty Baltiyskogo shita 1:500000 / ed.: F.P.Mitrofanov. Apatity: KFAN SSSR. 1994. 95 P. (in russian) 8. Haritonov L.Y. Structura i stratigraphia karelid vostoka Baltiskogo shita. M.: Nedra. 1966. 354 P. (in russian)
-
JPRS Report, Science & Technology, Japan
1988-05-06
Sought ( Nobuaki Teraoka; PUROMETEUSU, Nov 87) 62 IPCR Molecular Laser Uranium Enrichment Method Discussed (GENSHIRYOKU IINKAI GEPPO, Nov 87... Kobayashi ) Investigation of Tokyo University character of winter (Professor Tatsuo thunder on Japan Kawamura, Sea side by new Assistant...PUROMETEUSU in Japanese Nov 87 pp 78-81 [Article by Nobuaki Teraoka, Technology Development Division, Atomic Energy Bureau, Science and Technology Agency
-
Analysis of Hantaan Virus RNA: Evidence for a New Genus of Bunyaviridae
1983-01-01
accomplished by et aL, 1976). This characteristic distin- incubation of 50 pg of virion RNA with 50 guishes bunyaviruses from rhabdoviruses #Ci of cytidine 3...to that of mM HEPES, 8 mM dithiothreitol, 10 mM nucleocapsids from the rhabdovirus , ve- MI 3s, and 5 sM ATP (Sigma). Following sicular stomatitis...similar to those described for the bunya- +DNas 11,606 virus Uukuniemi (Ranki and Pettersson, +1 paM CT 5864 1975) or for the rhabdovirus VSV (Huang +5
-
Tomato Spotted Wilt Virus Particle Morphogenesis in Plant Cells
Kikkert, Marjolein; Van Lent, Jan; Storms, Marc; Bodegom, Pentcho; Kormelink, Richard; Goldbach, Rob
1999-01-01
A model for the maturation of tomato spotted wilt virus (TSWV) particles is proposed, mainly based on results with a protoplast infection system, in which the chronology of different maturation events could be determined. By using specific monoclonal and polyclonal antisera in immunofluorescence and electron microscopy, the site of TSWV particle morphogenesis was determined to be the Golgi system. The viral glycoproteins G1 and G2 accumulate in the Golgi prior to a process of wrapping, by which the viral nucleocapsids obtain a double membrane. In a later stage of the maturation, these doubly enveloped particles fuse to each other and to the endoplasmic reticulum to form singly enveloped particles clustered in membranes. Similarities and differences between the maturation of animal-infecting (bunya)viruses and plant-infecting tospoviruses are discussed. PMID:9971812
-
Conservation challenge: human-herbivore conflict in Chebera Churchura National Park, Ethiopia.
Datiko, Demeke; Bekele, Afework
2013-12-01
An investigation on human-herbivore conflict was carried out in CCNP between 2011 and 2012 in seven randomly selected villages (Chebera, Serri, Yora, Shita, Delba, Chuchra, Chewda) around the Park. A total of 312 household samples were identified for interview. Group discussion and field observation were also carried out. Among the respondents, the majority (83.9%) faced crop damage. African elephant (Loxodonta africana), Hippopotamus (Hippopotamus amphibious), African buffalo (Syncerus caffer), Desert warthog (Phacochoerus aethiopicus), Wild pig (Sus scrofa), Porcupine (Hystrix cristata), Vervet monkey (Cercopithecus aethiops) and Anubis baboon (Papio anubis) were identified as the most problematic animals in the area. However, buffalo, monkey and warthog were considered as the notorious pest. Crop damage and threats to human safety were the major problems encountered resulting in conflict between human and wildlife. Most respondents had a negative attitude towards the problem-posing animals. This will lead to a change in public attitude from one that supports wildlife conservation to sees wild herbivores as a threat and a potential negative consequence for wildlife conservation. Active measures have to be implemented to solve the problems and safeguard the future of the wildlife management in the park.
-
Reguera, Juan; Gerlach, Piotr; Rosenthal, Maria; Gaudon, Stephanie; Coscia, Francesca; Günther, Stephan; Cusack, Stephen
2016-01-01
Segmented negative strand RNA viruses of the arena-, bunya- and orthomyxovirus families uniquely carry out viral mRNA transcription by the cap-snatching mechanism. This involves cleavage of host mRNAs close to their capped 5′ end by an endonuclease (EN) domain located in the N-terminal region of the viral polymerase. We present the structure of the cap-snatching EN of Hantaan virus, a bunyavirus belonging to hantavirus genus. Hantaan EN has an active site configuration, including a metal co-ordinating histidine, and nuclease activity similar to the previously reported La Crosse virus and Influenza virus ENs (orthobunyavirus and orthomyxovirus respectively), but is more active in cleaving a double stranded RNA substrate. In contrast, Lassa arenavirus EN has only acidic metal co-ordinating residues. We present three high resolution structures of Lassa virus EN with different bound ion configurations and show in comparative biophysical and biochemical experiments with Hantaan, La Crosse and influenza ENs that the isolated Lassa EN is essentially inactive. The results are discussed in the light of EN activation mechanisms revealed by recent structures of full-length influenza virus polymerase. PMID:27304209
-
In Vitro Plant Regeneration from Commercial Cultivars of Soybean.
Raza, Ghulam; Singh, Mohan B; Bhalla, Prem L
2017-01-01
Soybean, a major legume crop, is the source of vegetable oil and protein. There is a need for transgenic approaches to breeding superior soybean varieties to meet future climate challenges. Efficient plant regeneration is a prerequisite for successful application of genetic transformation technology. Soybean cultivars are classified into different maturity groups based on photoperiod requirements. In this study, nine soybean varieties belonging to different maturity group were regenerated successfully from three different explants: half split hypocotyl, complete hypocotyl, and cotyledonary node. All the genotypes and explant types responded by producing adventitious shoots. Shoot induction potential ranged within 60-87%, 50-100%, and 75-100%, and regeneration rate ranged within 4.2-10, 2.7-4.2, and 2.6-10.5 shoots per explant using half split hypocotyl, complete hypocotyl, and cotyledonary explants, respectively, among all the tested genotypes. Bunya variety showed the best regeneration response using half split and complete hypocotyl explants and the PNR791 with cotyledonary node. The regenerated shoots were successfully rooted and acclimatized to glasshouse conditions. This study shows that commercial varieties of soybean are amenable to shoot regeneration with high regeneration frequencies and could be exploited for genetic transformation. Further, our results show no correlation between shoots regeneration capacity with the maturity grouping of the soybean cultivars tested.
-
The LANL hemorrhagic fever virus database, a new platform for analyzing biothreat viruses.
Kuiken, Carla; Thurmond, Jim; Dimitrijevic, Mira; Yoon, Hyejin
2012-01-01
Hemorrhagic fever viruses (HFVs) are a diverse set of over 80 viral species, found in 10 different genera comprising five different families: arena-, bunya-, flavi-, filo- and togaviridae. All these viruses are highly variable and evolve rapidly, making them elusive targets for the immune system and for vaccine and drug design. About 55,000 HFV sequences exist in the public domain today. A central website that provides annotated sequences and analysis tools will be helpful to HFV researchers worldwide. The HFV sequence database collects and stores sequence data and provides a user-friendly search interface and a large number of sequence analysis tools, following the model of the highly regarded and widely used Los Alamos HIV database [Kuiken, C., B. Korber, and R.W. Shafer, HIV sequence databases. AIDS Rev, 2003. 5: p. 52-61]. The database uses an algorithm that aligns each sequence to a species-wide reference sequence. The NCBI RefSeq database [Sayers et al. (2011) Database resources of the National Center for Biotechnology Information. Nucleic Acids Res., 39, D38-D51.] is used for this; if a reference sequence is not available, a Blast search finds the best candidate. Using this method, sequences in each genus can be retrieved pre-aligned. The HFV website can be accessed via http://hfv.lanl.gov.
-
In Vitro Plant Regeneration from Commercial Cultivars of Soybean
Raza, Ghulam; Singh, Mohan B.
2017-01-01
Soybean, a major legume crop, is the source of vegetable oil and protein. There is a need for transgenic approaches to breeding superior soybean varieties to meet future climate challenges. Efficient plant regeneration is a prerequisite for successful application of genetic transformation technology. Soybean cultivars are classified into different maturity groups based on photoperiod requirements. In this study, nine soybean varieties belonging to different maturity group were regenerated successfully from three different explants: half split hypocotyl, complete hypocotyl, and cotyledonary node. All the genotypes and explant types responded by producing adventitious shoots. Shoot induction potential ranged within 60–87%, 50–100%, and 75–100%, and regeneration rate ranged within 4.2–10, 2.7–4.2, and 2.6–10.5 shoots per explant using half split hypocotyl, complete hypocotyl, and cotyledonary explants, respectively, among all the tested genotypes. Bunya variety showed the best regeneration response using half split and complete hypocotyl explants and the PNR791 with cotyledonary node. The regenerated shoots were successfully rooted and acclimatized to glasshouse conditions. This study shows that commercial varieties of soybean are amenable to shoot regeneration with high regeneration frequencies and could be exploited for genetic transformation. Further, our results show no correlation between shoots regeneration capacity with the maturity grouping of the soybean cultivars tested. PMID:28691031
-
Defining appropriate methods for studying toxicities of trace metals in nutrient solutions.
Li, Zhigen; Wang, Peng; Menzies, Neal W; Kopittke, Peter M
2018-01-01
The use of inappropriate experimental conditions for examining trace metal phytotoxicity results in data of questionable value. The present study aimed to identify suitable parameters for study of phytotoxic metals in nutrient solutions. First, the literature was reviewed to determine the concentration of six metals (Cd, Cu, Hg, Ni, Pb, and Zn) from solution of contaminated soils. Next, the effects of pH, P, Cl, NO 3 , and four Fe-chelators were investigated by using thermodynamic modelling and by examining changes in root elongation rate of soybean (Glycine max cv. Bunya). The literature review identified that the solution concentrations of metals in soils were low, ranging from (µM) 0.069-11Cd, 0.19-15.8 Cu, 0.000027-0.000079 Hg, 1.0-8.7 Ni, 0.004-0.55 Pb, and 0.4-36.3 Zn. For studies in nutrient solution, pH should generally be low given its effects on solubility and speciation, as should the P concentration due to the formation of insoluble phosphate salts. The concentrations of Cl, NO 3 , and various chelators also influence metal toxicity through alteration of metal speciation. The nutrient solutions used to study metal toxicity should consider environmentally-relevant conditions especially for metal concentrations, with concentrations of other components added at levels that do not substantially alter metal toxicity. Copyright © 2017 Elsevier Inc. All rights reserved.
-
The LANL hemorrhagic fever virus database, a new platform for analyzing biothreat viruses
Kuiken, Carla; Thurmond, Jim; Dimitrijevic, Mira; Yoon, Hyejin
2012-01-01
Hemorrhagic fever viruses (HFVs) are a diverse set of over 80 viral species, found in 10 different genera comprising five different families: arena-, bunya-, flavi-, filo- and togaviridae. All these viruses are highly variable and evolve rapidly, making them elusive targets for the immune system and for vaccine and drug design. About 55 000 HFV sequences exist in the public domain today. A central website that provides annotated sequences and analysis tools will be helpful to HFV researchers worldwide. The HFV sequence database collects and stores sequence data and provides a user-friendly search interface and a large number of sequence analysis tools, following the model of the highly regarded and widely used Los Alamos HIV database [Kuiken, C., B. Korber, and R.W. Shafer, HIV sequence databases. AIDS Rev, 2003. 5: p. 52–61]. The database uses an algorithm that aligns each sequence to a species-wide reference sequence. The NCBI RefSeq database [Sayers et al. (2011) Database resources of the National Center for Biotechnology Information. Nucleic Acids Res., 39, D38–D51.] is used for this; if a reference sequence is not available, a Blast search finds the best candidate. Using this method, sequences in each genus can be retrieved pre-aligned. The HFV website can be accessed via http://hfv.lanl.gov. PMID:22064861
-
Novel RNA viruses within plant parasitic cyst nematodes
Ruark, Casey L.; Gardner, Michael; Mitchum, Melissa G.; Davis, Eric L.
2018-01-01
The study of invertebrate–and particularly nematode–viruses is emerging with the advancement of transcriptome sequencing. Five single-stranded RNA viruses have now been confirmed within the economically important soybean cyst nematode (SCN; Heterodera glycines). From previous research, we know these viruses to be widespread in greenhouse and field populations of SCN. Several of the SCN viruses were also confirmed within clover (H. trifolii) and beet (H. schachtii) cyst nematodes. In the presented study, we sequenced the transcriptomes of several inbred SCN populations and identified two previously undiscovered viral-like genomes. Both of these proposed viruses are negative-sense RNA viruses and have been named SCN nyami-like virus (NLV) and SCN bunya-like virus (BLV). Finally, we analyzed publicly available transcriptome data of two potato cyst nematode (PCN) species, Globodera pallida and G. rostochiensis. From these data, a third potential virus was discovered and called PCN picorna-like virus (PLV). PCN PLV is a positive-sense RNA virus, and to the best of our knowledge, is the first virus described within PCN. The presence of these novel viruses was confirmed via qRT-PCR, endpoint PCR, and Sanger sequencing with the exception of PCN PLV due to quarantine restrictions on the nematode host. While much work needs to be done to understand the biological and evolutionary significance of these viruses, they offer insight into nematode ecology and the possibility of novel nematode management strategies. PMID:29509804
-
Novel RNA viruses within plant parasitic cyst nematodes.
Ruark, Casey L; Gardner, Michael; Mitchum, Melissa G; Davis, Eric L; Sit, Tim L
2018-01-01
The study of invertebrate-and particularly nematode-viruses is emerging with the advancement of transcriptome sequencing. Five single-stranded RNA viruses have now been confirmed within the economically important soybean cyst nematode (SCN; Heterodera glycines). From previous research, we know these viruses to be widespread in greenhouse and field populations of SCN. Several of the SCN viruses were also confirmed within clover (H. trifolii) and beet (H. schachtii) cyst nematodes. In the presented study, we sequenced the transcriptomes of several inbred SCN populations and identified two previously undiscovered viral-like genomes. Both of these proposed viruses are negative-sense RNA viruses and have been named SCN nyami-like virus (NLV) and SCN bunya-like virus (BLV). Finally, we analyzed publicly available transcriptome data of two potato cyst nematode (PCN) species, Globodera pallida and G. rostochiensis. From these data, a third potential virus was discovered and called PCN picorna-like virus (PLV). PCN PLV is a positive-sense RNA virus, and to the best of our knowledge, is the first virus described within PCN. The presence of these novel viruses was confirmed via qRT-PCR, endpoint PCR, and Sanger sequencing with the exception of PCN PLV due to quarantine restrictions on the nematode host. While much work needs to be done to understand the biological and evolutionary significance of these viruses, they offer insight into nematode ecology and the possibility of novel nematode management strategies.
-
1990-06-01
An additional group of mice was injected with Propionibacterium acnes (70 mg/kg) as a positive control. As illustrated in Table 5 no significant...macrophage cytotoxicity was observed in any of the groups. Macrophages from mice injected with P. acnes exhibited the expectected anti-tumor cytotoxic...peritoneal macrophages cytotoxic. In contrast P. acnes was highly effective. C.3.13 Peritoneal exudate cell phagocytosis following treatment with free or
-
1991-08-01
Group 3 on days 2, 4, and 6; and Group 4 on days 2, 4, 6 and 8. An additional group of mice was injected with Propionibacterium acnes (70 mg/kg) as a...with P. acnes exhibited the expected anti-tumor cytotoxic activity (data not shown). Similar results were obtained with CL 246738 (Table 14). C.2.1.4...nor liposome encapsulated drug was able to render peritoneal macrophages cytotoxic. In contrast P. acnes was highly effective. C.2.3.13 Peritoneal
-
1989-05-22
potentially useful antivirals (e... S-HMPA, selenazole, WIN 5177, arildone, phosphonoformic acid ) are currently under investigation. There are, however...by increasing dosage, this often results in toxicity. For example, a number of antiviral agents (ribavirin, adenine arabinoside, phosphonoformic acid ...Three possibilities exist for the activation of carrier bound drugs: 1) endocytosis and release in acidic endosomes; 2) extracellular activation at cell