These are representative sample records from Science.gov related to your search topic.
For comprehensive and current results, perform a real-time search at Science.gov.
1

SP Fonts  

NSDL National Science Digital Library

If you're looking for funky fonts to include in Web designs, papers, or posters, SP Fonts may be your answer. Scholars Press (SP) Fonts "are a set of simple, public domain fonts" designed for print and non-commercial Web use. This site currently offers eight public domain fonts that may be downloaded and used free of charge. Three of the fonts are Hebrew/Aramaic fonts: "SPTiberian (a standard Hebrew font), SPDamascus (a thinner font with Palestinian as well as Tiberian vowel points), and SPEzra (a simple, fixed-width Hebrew font)." Two are Greek fonts: "SPIonic (a more complete Greek font) and SPDoric (a simpler, uncial font)." Other fonts include "SPEdessa (a Syriac Estrangela font), SPAchmim (a Coptic font), and SPAtlantis (a transliteration font that includes diacriticals and other special characters that allow the representation of numerous Indo-European, Semitic, and other languages"-- available in both Roman and Italic type). All fonts are TrueType fonts and are compatible with PC (Windows) and Mac computers. In addition, each font has a .readme file that explains the standard keyboard mapping used by the font. Although the fonts are free to the public, the Web site requests permission from the copyright holder before including the typefaces in commercial electronic products.

2000-01-01

2

SP100 progress  

Microsoft Academic Search

SP-100 technology satisfies the emerging high power requirements that will enable future space missions and is readily adaptable to provide base power for lunar and Martian surface applications. The SP-100 nuclear power reactor can also be coupled to various static and dynamic power conversion systems as they achieve technology readiness. This unique capability expands SP-100's applicability into the range needed

J. S. Armijo; A. T. Josloff; H. S. Bailey; D. N. Matteo

1991-01-01

3

Dihydroramulosin from Botrytis sp.  

PubMed

Botrytis sp., isolated from the inner bark of the Pacific yew, Taxus brevifolia, was shown to produce ramulosin (1), 6-hydroxyramulosin (2), and the new compound 8-dihydroramulosin (3). The structure of dihydroramulosin was deduced from the NMR spectra and confirmed by chemical conversion from ramulosin. PMID:9784167

Stierle, D B; Stierle, A A; Kunz, A

1998-10-01

4

Homoptera: Cicadidae Magicicada sp.  

E-print Network

Homoptera: Cicadidae Magicicada sp. Other Common names Periodical cicadas are also commonly called the 17-year cicada, 13-year cicada, or locusts. The name "locust" is misleading because it applies to migratory grasshoppers. Plants Attacked In Virginia both the 17-and 13-year cicadas damage many ornamental

Liskiewicz, Maciej

5

SP100 reactor design  

Microsoft Academic Search

The SP-100 space reactor power system is being designed and developed as part of the Ground Engineering System (GES) contract between General Electric Company as the system developer and the Department of Energy. Other key participants in the GES program include Westinghouse Hanford Company (site operator), Los Alamos National Laboratory (fuel development and production), Oak Ridge National Laboratory (materials), and

J. S. Armijo; J. Atwell; P. R. Pluta; M. A. Smith; E. R. Solorzano

1987-01-01

6

SP mountain data analysis  

NASA Technical Reports Server (NTRS)

An analysis of synthetic aperture radar data of SP Mountain was undertaken to demonstrate the use of digital image processing techniques to aid in geologic interpretation of SAR data. These data were collected with the ERIM X- and L-band airborne SAR using like- and cross-polarizations. The resulting signal films were used to produce computer compatible tapes, from which four-channel imagery was generated. Slant range-to-ground range and range-azimuth-scale corrections were made in order to facilitate image registration; intensity corrections were also made. Manual interpretation of the imagery showed that L-band represented the geology of the area better than X-band. Several differences between the various images were also noted. Further digital analysis of the corrected data was done for enhancement purposes. This analysis included application of an MSS differencing routine and development of a routine for removal of relief displacement. It was found that accurate registration of the SAR channels is critical to the effectiveness of the differencing routine. Use of the relief displacement algorithm on the SP Mountain data demonstrated the feasibility of the technique.

Rawson, R. F.; Hamilton, R. E.; Liskow, C. L.; Dias, A. R.; Jackson, P. L.

1981-01-01

7

Laser Sculpting of Atomic sp, sp(2) , and sp(3) Hybrid Orbitals.  

PubMed

Atomic sp, sp(2) , and sp(3) hybrid orbitals were introduced by Linus Pauling to explain the nature of the chemical bond. Quantum dynamics simulations show that they can be sculpted by means of a selective series of coherent laser pulses, starting from the 1s orbital of the hydrogen atom. Laser hybridization generates atoms with state-selective electric dipoles, opening up new possibilities for the study of chemical reaction dynamics and heterogeneous catalysis. PMID:25257703

Liu, Chunmei; Manz, Jörn; Yang, Yonggang

2015-01-12

8

SP100 flight safety tests  

Microsoft Academic Search

The safety test program to be conducted as part of the SP-100 Power System Qualification Program is described. Safety tests needed to establish SP-100 technology readiness are identified through consideration of uncertainties in analytical predictions. Critical experiments already completed provide sufficient confidence in the technology supporting criticality predictions under accident conditions. Plans for the future include: testing of safety rod

Mark I. Temme; Neil W. Brown; Michael A. Smith

1993-01-01

9

DADiSP processing guide  

NASA Technical Reports Server (NTRS)

A guide for DADiSP software, intended for use by the Lambda Point Experiment (LPE) Team during and after the United States Microgravity Payload (USMP)-1 mission, is presented. DADiSP is a Data Analysis and Display Software developed and marketed by DSP Development Corporation, Cambridge, Massachusetts. This guide is intended to be used in addition to the DADiSP Worksheet User Manual and Reference Manual which are supplied by the company with the software. Technical support for DADiSP is available from DSP at (617) 577-1133. Access to DADiSP on Acceleration Characterization and Analysis Project (ACAP) EGSE is being provided to the LPE team during USMP-1 for off-line processing of SAMS data.

Rogers, Melissa J. B.

1993-01-01

10

Allochromatium renukae sp. nov.  

PubMed

An ovoid to rod-shaped, phototrophic, purple sulfur bacterium, designated strain JA136(T), was isolated in pure culture from brackish water near Kakinada, India, in a medium that contained 2 % NaCl (w/v). Cells were Gram-negative and motile by means of a single polar flagellum. Strain JA136(T) had no absolute salt requirement for growth but was able to tolerate up to 4 % NaCl (w/v). Intracellular photosynthetic membranes were of the vesicular type. Bacteriochlorophyll a and the carotenoid lycopene of the rhodopinal series were present as photosynthetic pigments. Strain JA136(T) was able to grow photolithoautotrophically and photolithoheterotrophically. There was no vitamin requirement for growth of strain JA136(T). Phylogenetic analysis on the basis of 16S rRNA gene sequences indicated that strain JA136(T) clustered with species of the genus Allochromatium in the class Gammaproteobacteria. Strain JA136(T) showed highest 16S rRNA gene sequence similarity with the type strains of Allochromatium vinosum (97.0 %), Allochromatium minutissimum (95.8 %) and Allochromatium warmingii (90.0 %). Based on 16S rRNA gene sequence similarity data and morphological and physiological characteristics, strain JA136(T) was sufficiently distinct from recognized Allochromatium species to be described as representing a novel species of the genus, for which the name Allochromatium renukae sp. nov. is proposed. The type strain is JA136(T) (=JCM 14262(T) =DSM 18713(T)). PMID:18218939

Anil Kumar, P; Srinivas, T N R; Sasikala, Ch; Ramana, Ch V

2008-02-01

11

Streptomyces muensis sp. nov.  

PubMed

A strain of Streptomyces, MBRL 179(T), isolated from a sample from a Limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain formed a monophyletic clade with Streptomyces spinoverrucosus NBRC 14228(T) (16S rRNA gene sequence similarity of 99.3 %) in the Neighbour-joining tree. DNA-DNA hybridization experiment gave a DNA-DNA relatedness value of 34.7 % between MBRL 179(T) and S. spinoverrucosus NBRC 14228(T). Strain MBRL 179(T) contained LL-diaminopimelic acid, xylose, glucose, and mannose in the whole cell-wall hydrolysates along with small amount of ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, with other unknown phospholipids and aminophospholipid. MK-9(H6), MK-9(H8) and MK-9(H4) were the predominant menaquinones detected. The major fatty acids were anteiso-C16:0 (28.1 %), iso-C16:0 (20.3 %), C16:0 (9.4 %) and anteiso-C17:0 (8.3 %). The G+C content of the genomic DNA was 71.1 %. Based on the polyphasic experiment results, the strain MBRL 179(T) merits recognition as a representative of a novel species of the genus Streptomyces for which the name Streptomyces muensis sp. nov. is proposed; the type strain is MBRL 179(T) (=JCM 17576(T) = KCTC 29124(T)). PMID:24037482

Ningthoujam, Debananda S; Nimaichand, Salam; Ningombam, Dollyca; Tamreihao, K; Li, Li; Zhang, Yong-Guang; Cheng, Juan; Liu, Min-Jiao; Li, Wen-Jun

2013-12-01

12

SP-100 Advanced Technology Program  

NASA Technical Reports Server (NTRS)

The goal of the triagency SP-100 Program is to develop long-lived, compact, lightweight, survivable nuclear reactor space power systems for application to the power range 50 kWe to 1 MWe. The successful development of these systems should enable or significantly enhance many of the future NASA civil and commercial missions. The NASA SP-100 Advanced Technology Program strongly augments the parallel SP-100 Ground Engineering System Development program and enhances the chances for success of the overall SP-100 program. The purpose of this paper is to discuss the key technical elements of the Advanced Technology Program and the progress made in the initial year and a half of the project.

Sovie, Ronald J.

1987-01-01

13

SP-100 advanced technology program  

SciTech Connect

The goal of the triagency SP-100 Program is to develop long-lived, compact, lightweight, survivable nuclear reactor space power systems for application to the power range 50 kWe to 1 MWe. The successful development of these systems should enable or significantly enhance many of the future NASA civil and commercial missions. The NASA SP-100 Advanced Technology Program strongly augments the parallel SP-100 Ground Engineering System Development program and enhances the chances for success of the overall SP-100 program. The purpose of this paper is to discuss the key technical elements of the Advanced Technology Program and the progress made in the initial year and a half of the project.

Sovie, R.J.

1987-01-01

14

Actinoplanes couchii sp. nov.  

PubMed

A Gram-positive bacterium, strain GW8-1761(T), was isolated from soil close to the Marmore waterfalls, Terni, Italy. 16S rRNA gene sequence similarity studies showed that strain GW8-1761(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes italicus JCM 3165(T) (98.9 %), A. rectilineatus IFO 13941(T) (98.5 %), A. palleronii JCM 7626(T) (97.8 %), A. utahensis IFO 13244(T) (97.6 %) and A. cyaneus DSM 46137(T) (97.6 %). Strain GW8-1761(T) could be distinguished from any other Actinoplanes species with validly published names by 16S rRNA gene sequence similarity values of less than 97.5 %. Chemotaxonomic data [major menaquinone MK-9(H(4)); major polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol, with phosphatidylcholine and aminoglycolipids absent; major fatty acids C(15 : 0), C(16 : 0), C(16 : 0) iso, C(17 : 1)omega8c and summed feature 3 (C(16 : 1)omega7c and/or C(15 : 0) iso 2-OH)] supported the affiliation of strain GW8-1761(T) to the genus Actinoplanes. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW8-1761(T) from the most closely related species. Strain GW8-1761(T) therefore merits species status, and we propose the name Actinoplanes couchii sp. nov., with the type strain GW8-1761(T) (=DSM 45050(T)=CIP 109316(T)). PMID:17392194

Kämpfer, Peter; Huber, Birgit; Thummes, Kathrin; Grün-Wollny, Iris; Busse, Hans-Jürgen

2007-04-01

15

Marmoricola korecus sp. nov.  

PubMed

A novel actinomycete, designated strain Sco-A36(T), was isolated from volcanic ash. Cells were aerobic, Gram-stain-positive, oxidase-negative, catalase-positive, non-motile cocci; colonies were yellow-coloured, smooth, entire and convex. Phylogenetic analysis showed that the organism formed a distinct phyletic line within the radiation of the genus Marmoricola. Its closest phylogenetic neighbours were Marmoricola aurantiacus DSM 12652(T) (97.9?% 16S rRNA gene sequence similarity), Marmoricola scoriae Sco-D01(T) (97.9?%), Marmoricola aequoreus SST-45(T) (97.4?%) and Marmoricola bigeumensis MSL-05(T) (96.3?%). The diagnostic diamino acid in the cell walls was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H(4)). The polar lipids contained phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The predominant fatty acids were C(16?:?0), C(17?:?1)?8c, C(18?:?1)?9c and C(16?:?1)?7c and/or iso-C(15?:?0) 2-OH. The DNA G+C content of strain Sco-A36(T) was 71.0 mol%. Levels of DNA-DNA relatedness between the new isolate and the type strains of recognized species of the genus Marmoricola were 4.9-29.2?%. The phenotypic and DNA-DNA hybridization data presented here strongly suggest that strain Sco-A36(T) represents a novel species of the genus Marmoricola, for which the name Marmoricola korecus sp. nov. is proposed. The type strain is Sco-A36(T) (?=?KCTC 19596(T)?=?DSM 22128(T)). PMID:20693360

Lee, Soon Dong; Lee, Dong Wan; Ko, Young-Hwan

2011-07-01

16

Roseospira visakhapatnamensis sp. nov. and Roseospira goensis sp. nov.  

PubMed

Two Gram-negative, vibrioid, phototrophic, purple non-sulfur strains, JA131T and JA135T, were isolated from marine habitats. Strain JA131T is non-motile but strain JA135T is motile by means of a pair of monopolar flagella. Both strains have an obligate requirement for NaCl for growth. The intracellular photosynthetic membranes of the two novel strains are of the vesicular type. Bacteriochlorophyll a and probably rhodovibrine are present as photosynthetic pigments. Niacin, thiamine and p-aminobenzoic acid are required as growth factors for both novel strains. Based on 16S rRNA gene sequence analysis, morphological and physiological characteristics, strains JA131T and JA135T are significantly different from each other and from other species of the genus Roseospira and thus represent two novel species for which the names Roseospira visakhapatnamensis sp. nov. and Roseospira goensis sp. nov. are proposed, respectively. The type strain of Roseospira visakhapatnamensis sp. nov. is JA131T (=ATCC BAA-1365T=JCM 14190T) and the type strain of Roseospira goensis sp. nov. is JA135T (=ATCC BAA-1364T=JCM 14191T). PMID:17978198

Kalyan Chakravarthy, S; Srinivas, T N R; Anil Kumar, P; Sasikala, Ch; Ramana, Ch V

2007-11-01

17

Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera  

PubMed Central

We previously discovered a symbiotic lactic acid bacterial (LAB) microbiota in the honey stomach of the honeybee Apis mellifera. The microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. 16S rRNA gene sequence analyses and phenotypic and genetic characteristics revealed that the phylotypes isolated represent seven novel species. One grouped with Lactobacillus kunkeei and the others belong to the Lactobacillus buchneri and Lactobacillus delbrueckiisubgroups of Lactobacillus. We propose the names Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov. for these novel species, with the respective type strains being Fhon13NT (?=?DSM 26257T?=?CCUG 63287T), Bin4NT (?=?DSM 26254T?=?CCUG 63291T), Hon2NT (?=?DSM 26255T?=?CCUG 63289T), Hma8NT (?=?DSM 26256T?=?CCUG 63629T), Hma2NT (?=?DSM 26263T?=?CCUG 63633T), Bma5NT (?=?DSM 26265T?=?CCUG 63301T) and Biut2NT (?=?DSM 26262T?=?CCUG 63631T). PMID:24944337

Alsterfjord, Magnus; Nilson, Bo; Butler, Èile; Vásquez, Alejandra

2014-01-01

18

Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera.  

PubMed

We previously discovered a symbiotic lactic acid bacterial (LAB) microbiota in the honey stomach of the honeybee Apis mellifera. The microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. 16S rRNA gene sequence analyses and phenotypic and genetic characteristics revealed that the phylotypes isolated represent seven novel species. One grouped with Lactobacillus kunkeei and the others belong to the Lactobacillus buchneri and Lactobacillus delbrueckii subgroups of Lactobacillus. We propose the names Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov. for these novel species, with the respective type strains being Fhon13N(T) (?=?DSM 26257(T)?=?CCUG 63287(T)), Bin4N(T) (?=?DSM 26254(T)?=?CCUG 63291(T)), Hon2N(T) (?=?DSM 26255(T)?=?CCUG 63289(T)), Hma8N(T) (?=?DSM 26256(T)?=?CCUG 63629(T)), Hma2N(T) (?=?DSM 26263(T)?=?CCUG 63633(T)), Bma5N(T) (?=?DSM 26265(T)?=?CCUG 63301(T)) and Biut2N(T) (?=?DSM 26262(T)?=?CCUG 63631(T)). PMID:24944337

Olofsson, Tobias C; Alsterfjord, Magnus; Nilson, Bo; Butler, Eile; Vásquez, Alejandra

2014-09-01

19

Argonne's SpEC Module  

SciTech Connect

Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

Harper, Jason

2014-05-05

20

Argonne's SpEC Module  

ScienceCinema

Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

Harper, Jason

2014-06-05

21

The Sp(1)-Kepler problems  

SciTech Connect

Let n{>=}2 be a positive integer. To each irreducible representation {sigma} of Sp(1), an Sp(1)-Kepler problem in dimension (4n-3) is constructed and analyzed. This system is superintegrable, and when n=2 it is equivalent to a generalized MICZ-Kepler problem in dimension of 5. The dynamical symmetry group of this system is O-tilde*(4n) with the Hilbert space of bound states H({sigma}) being the unitary highest weight representation of O*-tilde(4n) with highest weight, (-1,{center_dot}{center_dot}{center_dot},-1,-(1+{sigma})), which occurs at the rightmost nontrivial reduction point in the Enright-Howe-Wallach classification diagram for the unitary highest weight modules. Here {sigma} is the highest weight of {sigma}. Furthermore, it is shown that the correspondence {sigma}{r_reversible}H({sigma}) is the theta-correspondence for dual pair (Sp(1),O*(4n))subset Sp(8n,R)

Meng Guowu [Department of Mathematics, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon (Hong Kong)

2009-07-15

22

SP100 control drive development  

Microsoft Academic Search

The SP-100 is an electrical generating nuclear power system for space operation. This paper describes the nuclear reactor control systems and the methods used to assure reliable performance for the 10 year design life. Reliable performance is achieved by redundancy and by selecting highly reliable components and design features. Reliability is quantified by analysis using established reliability data. Areas lacking

Thomas E. Gleason; A. Richard Gilchrist; Gary B. Schuster

1993-01-01

23

Manufacturing SP100 rhenium tubes  

Microsoft Academic Search

A process for producing high quality, thin walled, wrought, rhenium tubing was successfully developed and qualified in the SP-100 fuel fabrication program. Rhenium was selected for the fuel-cladding barrier versus tungsten because of the cold workability and nuclear characteristics of rhenium. Several tube fabricating processes including swaging, drawing, and extruding sintered tube shells and chemical vapor deposition were evaluated before

Edwin D. Sayre; Thomas J. Ruffo

1992-01-01

24

SP100 control system modeling  

Microsoft Academic Search

SP-100 Control Systems modeling was done using a thermal hydraulic transient analysis model called ARIES-S. The ARIES-S Computer Simulation provides a basis for design, integration and analysis of the reactor including the control and protection systems. It is a modular digital computer simulation written in FORTRAN that operates interactively in real time on a VAX minicomputer.

R. A. Meyer; F. J. Halfen; A. D. Alley

1987-01-01

25

Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov.  

PubMed

A polyphasic study was carried out to establish the taxonomic positions of six strains isolated from diverse soil samples and provisionally assigned to the genus Kitasatospora. The isolates were found to have chemical and morphological properties consistent with their classification as Kitasatospora strains. Direct 16S rRNA gene sequence data confirmed the taxonomic position of the strains following the generation of phylogenetic trees by using three tree-making algorithms. Five of the isolates were considered to merit species status using complementary genotypic and phenotypic data. These organisms were designated Kitasatospora arboriphila sp. nov. (HKI 0189(T)=2291-120(T)=DSM 44785(T)=NCIMB 13973(T)), Kitasatospora gansuensis sp. nov. (HKI 0314(T)=2050-015(T)=DSM 44786(T)=NCIMB 13974(T)), Kitasatospora nipponensis sp. nov. (HKI 0315(T)=2148-013(T)=DSM 44787(T)=NCIMB 13975(T)), Kitasatospora paranensis sp. nov. (HKI 0190(T)=2292-041(T)=DSM 44788(T)=NCIMB 13976(T)) and Kitasatospora terrestris sp. nov. (HKI 0186(T)=2293-012(T)=DSM 44789(T)=NCIMB 13977(T)). The remaining organism, isolate HKI 0316 (=2122-022=DSM 44790=NCIMB 13978), was considered to be a strain of Kitasatospora kifunensis on the basis of 16S rRNA gene sequence, DNA-DNA relatedness and phenotypic data. PMID:15545445

Groth, Ingrid; Rodríguez, Carlos; Schütze, Barbara; Schmitz, Petra; Leistner, Eckhard; Goodfellow, Michael

2004-11-01

26

SP100 space reactor design  

Microsoft Academic Search

The SP100 space nuclear reactor was designed for use as an orbital power supply, lunar or Martian surface power station, and power supply for nuclear electric propulsion, with a scaleable power range of 10's kWe to 100's kWe. The original mission was an orbital power supply for the United State's (U.S.) Strategic Defense Initiative (SDI) of the 1980s. Although the

Scott F. Demuth

2003-01-01

27

SP100 Control System Design  

Microsoft Academic Search

Recent work in lower power generic early applications for the SP-100 have resulted in control system design simplification for a 20 kWe design with thermoelectric power conversion. This paper presents the non-mission-dependent control system features for this design. The control system includes a digital computer based controller, dual purpose control rods and drives, temperature sensors, and neutron flux monitors. The

Jaikaran N. Shukla; Frank J. Halfen; Glen V. Brynsvold; Akbar Syed; Thomas J. Jiang; Kwok K. Wong; Robert L. Otwell

1994-01-01

28

Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov. Isolated from Clinical Specimens of Human Origin?  

PubMed Central

Three groups of previously unknown gram-positive, anaerobic, coccus-shaped bacteria were characterized using phenotypic and molecular taxonomic methods. Phenotypic and genotypic data demonstrate that these organisms are distinct, and each group represents a previously unknown subline within Clostridium cluster XIII. Two groups are most closely related to Peptoniphilus harei in the genus Peptoniphilus, and the other group is most closely related to Anaerococcus lactolyticus in the genus Anaerococcus. Based on the findings, three novel species, Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov., are proposed. The type strains of Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov. are WAL 10418T (= CCUG 53341T = ATCC BAA-1383T), WAL 12922T (= CCUG 53342T = ATCC BAA-1384T), and WAL 17230T (= CCUG 53340T = ATCC BAA-1385T), respectively. PMID:17428937

Song, Yuli; Liu, Chengxu; Finegold, Sydney M.

2007-01-01

29

Cultivation of Monoraphidium sp., Chlorella sp. and Scenedesmus sp. algae in Batch culture using Nile tilapia effluent.  

PubMed

Monoraphidium sp., Chlorella sp. and Scenedesmus sp. algae were cultured in three volumes of Tilapia Effluent Medium (TEM) in comparison with the Bold Basal Medium (BBM) (Nichols and Bold, 1965). Specific growth rate (?'), biomass dry productivity (Q), volumetric productivity (Qv) as well as lipid and protein content were measured. Then, volumetric productivities for both lipids and proteins were calculated (QVL and QVP). In Scenedesmus sp., BBM produced higher ?' and Qv than TEM in 1.5L volume. Chlorella sp. showed a higher QVL for BBM than TEM. Any observed difference in protein or lipid productivities among volumes was in favor of a greater productivity for 1.5L volume. Even when TEM had a larger protein content in Chlorella sp. than BBM, QVP was not different. Current results imply that TEM can be used as an alternative growth medium for algae when using Batch cultures, yet productivity is reduced. PMID:24736090

Guerrero-Cabrera, Luis; Rueda, José A; García-Lozano, Hiram; Navarro, A Karin

2014-06-01

30

Postirradiation examination results from SP1  

Microsoft Academic Search

This paper describes the postirradiation examination results from several of the fuel pins irradiated in the SP-1 test. The SP-1 test is the first of two tests irradiated in EBR-II to be examined. These tests are designed to provide a direct comparison of the performance potential of UOâ and UN fuel pins under conditions anticipated for the SP-100 reactor. In

R. A. Karnesky; R. E. Mason

1986-01-01

31

Environmental conditions affecting exopolysaccharide production by Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp  

Microsoft Academic Search

Three different chromium-resistant microorganisms (Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.) were tested with regard to their EPS production at different pH levels, temperatures, Cr(VI) concentrations, and incubation periods. The optimum pH level was 7 for P. aeruginosa and Micrococcus sp., while it was 8 for Ochrobactrum sp. according to the highest EPS amount at 100mg\\/L Cr(VI) concentration. The highest

Nur Koçberber K?l?ç; Gönül Dönmez

2008-01-01

32

Keratitis due to Chaetomium sp.  

PubMed Central

Aim. To describe keratitis due to Chaetomium sp. occurring in a 65-year-old woman who presented with a corneal ulcer with hypopyon of the right eye with a history of trauma by vegetable matter. Method. Multiple scrapings were obtained from the ulcer. A lactophenol cotton blue wet mount and a Gram-stained smear of the scrapings were made. Scrapings were also inoculated onto various culture media. Results. Direct microscopy of corneal scrapings revealed moderate numbers of septate fungal hyphae. Greenish-yellow-coloured fungal colonies with aerial mycelium were observed in culture of the corneal scrapes. On the basis of colony characteristics and conidial structure, the fungal isolate was identified as Chaetomium sp. The patient was treated with topical natamycin (5%) hourly and cyclopentolate 1% drops 3 times a day. After 4 weeks of therapy, the hypopyon had disappeared, the epithelial defect had healed, and the stromal infiltration had almost completely resolved; the visual acuity of the eye improved from hand movements to (1/2)/60. Conclusion. Fungi of the genus Chaetomium, which are rare causes of human disease (systemic mycosis, endocarditis, subcutaneous lesions), may also cause ocular lesions. PMID:22606471

Kaliamurthy, Jayaraman; Kalavathy, Catti Munuswamy; Nelson Jesudasan, Christadoss Arul; Thomas, Philip A.

2011-01-01

33

GAMBUSIA INFANS,sp. nov, Salamanca.Xlexico. NOTROPIS CHIHUAHUA, sp. nov. Salamanca.Xlexico.  

E-print Network

GAMBUSIA INFANS,sp. nov, Salamanca.Xlexico. NOTROPIS CHIHUAHUA, sp. nov. Salamanca.Xlexico. EVARRA EIGENMANNI, gen. et sp. nov, Mexico City, Mexico. NOTROPIS AZTECUS, ep. nov. Mexico City, JUexieo. CHIROSTOMA Grande'. Moxostoma austrinum. Rio Lerma. Family Cypriuidre. Notropis Iutreusis. Rio Couches. Notropis

34

Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov.  

PubMed

Two thermophilic bacilli were isolated from mud and water samples of the Ayder and Kestanbol hot springs in the provinces of Rize and Canakkale, respectively, in Turkey. Strains AB04T and K4T were sporulating, Gram-positive, rod-shaped bacteria. These isolates were moderately thermophilic (with an optimum temperature for growth of 50-55 degrees C), facultative anaerobes able to grow on a wide range of carbon sources including d-glucose, d-raffinose, d-sucrose, D-xylose, D-fructose, L-arabinose, maltose, D-mannose and D-mannitol. Analysis of the 16S rRNA gene sequences showed that these isolates resembled Anoxybacillus flavithermus DSM 2641T and Anoxybacillus gonensis NCIMB 13933T. DNA-DNA hybridization data revealed that thermophilic isolate AB04T has only 51.2 % relatedness to A. flavithermus, 45.1 % relatedness to Anoxybacillus pushchinoensis and 68.6 % relatedness to A. gonensis. Thermophilic isolate K4T showed only 60.4 % relatedness to A. flavithermus, 42.9 % relatedness to A. pushchinoensis and 38.5 % relatedness to A. gonensis. On the basis of the DNA-DNA hybridization data, isolates AB04T and K4T are not related to A. flavithermus DSM 2641T, A. pushchinoensis DSM 12423T or A. gonensis NCIMB 13933T at the species level, but show relatedness to one another of 40.5 %. On the basis of the data presented, it is proposed that strains AB04T (= NCIMB 13972T = NCCB 100050T) and K4T (= NCIMB 13971T = NCCB 100051T) be designated as the type strains of Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov., respectively. PMID:15388701

Dulger, Sabriye; Demirbag, Zihni; Belduz, Ali Osman

2004-09-01

35

_q .. SP-6102 -" IN SYSTEMS ENGINEERING  

E-print Network

_¢q .. SP-6102 -" READINGS IN SYSTEMS ENGINEERING Edited by Francis T. Hoban and William M. Lawbaugh co ! (NASA-SP-6102) REAOINGS IN SYSTEMS ENGINEERING (NASa) 215 p N93-24678 --THRU-- N93-24693 Unclas H1/31 0158570 #12;.J T ,j J #12;READINGS IN SYSTEMS ENGINEERING Edited by Francis T. Hoban

Rhoads, James

36

SP100 start-up control strategy  

Microsoft Academic Search

A control analysis was performed to evaluate the reference and two alternative reactor start-up control strategies for the SP-100, using a detailed nonlinear model of the reactor. The analysis results show that the reference control strategy for the SP-100 adequately meets the current requirements. The two alternative control strategies provide tighter control than the reference strategy. Use of the measured

Raymond A. Meyer; Sang K. Rhow; Kwok K. Wong; Frank J. Halfen

1991-01-01

37

SP100 liquid metal test loop design  

Microsoft Academic Search

The SP-100 Power System Qualification (PSO) program validates the technology readiness of the SP-100 Generic Flight System (GFS). As part of the PSQ, the GFS reactor, heat transport and power generation systems are being validated, by test, in high temperature liquid metal test loops. The liquid metal test loop program consists of two test loops. The first, a natural circulation

T. Ted Fallas; Gordon B. Kruger; Frank R. Wiltshire; Grant C. Jensen; Harold Clay; Hugh A. Upton; Robert E. Gamble; Christian Kjaer-Olsen; Keith Lee

1992-01-01

38

SP100 nuclear subsystem hardware and testing  

Microsoft Academic Search

A development status and prospective performance improvement assessment is presented for component technologies associated with the SP-100 spacecraft nuclear power system. These components encompass fuel pellets, materials and their fabrication methods, reactivity-control mechanisms, and sensors. The SP-100 system can be configured to support orbital, interplanetary, and surface power applications over a wide net-electrical-power range.

Philip R. Pluta; Anthony J. Bryhan; Samuel Kaplan; Raymond A. Meyer; Michael R. Schrag; Robert Yaspo

1992-01-01

39

SP100 early flight mission designs  

Microsoft Academic Search

Two systems design options are under consideration for the use of thermoelectric technology in conjunction with the SP-100 nuclear reactor for early implementation spaceflights. Attention is presently given to the option that employs radiatively coupled Radioisotope Thermoelectric Generator (RTG) unicouples. The flight-demonstrated status of RTG technology is consistent with a 1996 lauch date; the use of the SP-100 reactor ensures

N. F. Shepard; R. E. Biddiscombe; T. S. Chan; N. A. Deane; A. S. Kirpich; R. Murata; R. Protsik; M. A. Smith; J. D. Stephen

1992-01-01

40

Differences in nutrient uptake capacity of the benthic filamentous algae Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. under varying N/P conditions.  

PubMed

The N/P ratio of wastewater can vary greatly and directly affect algal growth and nutrient removal process. Three benthic filamentous algae species Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. were isolated from a periphyton bioreactor and cultured under laboratory conditions on varying N/P ratios to determine their ability to remove nitrate and phosphorus. The N/P ratio significantly influenced the algal growth and phosphorus uptake process. Appropriate N/P ratios for nitrogen and phosphorus removal were 5-15, 7-10 and 7-20 for Cladophora sp., Klebsormidium sp. and Pseudanabaena sp., respectively. Within these respective ranges, Cladophora sp. had the highest biomass production, while Pseudanabaena sp. had the highest nitrogen and phosphorus contents. This study indicated that Cladophora sp. had a high capacity of removing phosphorus from wastewaters of low N/P ratio, and Pseudanabaena sp. was highly suitable for removing nitrogen from wastewaters with high N/P ratio. PMID:25544498

Liu, Junzhuo; Vyverman, Wim

2015-03-01

41

Bioaccumulation of total and methyl mercury in three earthworm species (Drawida sp., Allolobophora sp., and Limnodrilus sp.).  

PubMed

We determined total and methyl mercury contents in soil, three earthworm species and their vomitus to study the species-specific differences of mercury bioconcentration in Huludao City, a heavily polluted region by chlor-alkali and nonferrous metal smelting industry in Liaoning Province, northeast China. Total and methyl mercury contents were 7.20 mg/kg and 6.94 ng/g in soil, 1.43 mg/kg and 43.03 ng/g in Drawida sp., 2.80 mg/kg and 336.52 ng/g in Alolobophora sp., respectively. Total mercury contents were 0.966 mg/kg in Drawida sp. vomitus and 4.979 mg/kg in Alolobophora sp. vomitus, respectively. Total mercury contents in earthworms and their vomitus were significantly species-specific different and were both in decreasing with earthworms body lengths, which might due to the growth dilution. Among the soil, earthworms and their vomitus, total mercury contents were in the order of soil > earthworms > earthworm vomitus. Methyl mercury was about 3.01% of total mercury in Drawida sp., 12.02% of total mercury in Alolobophora sp., respectively. It suggested that mercury was mostly in inorganic forms in earthworms. Bioaccumulation factors of methyl mercury from soil to earthworms were much higher than those of total mercury, which suggested that methyl mercury might be more easily absorbed by and accumulated in earthworms because of its lipid solubility. PMID:19779655

Zhang, Zhong Sheng; Zheng, Dong Mei; Wang, Qi Chao; Lv, Xian Guo

2009-12-01

42

Relationship between the Unicellular Red Alga Porphyridium sp. and Its Predator, the Dinoflagellate Gymnodinium sp  

PubMed Central

Contamination of algae cultivated outdoors by various microorganisms, such as bacteria, fungi, algae, and protozoa, can affect growth and product quality, sometimes causing fast collapse of the cultures. The main contaminant of Porphyridium cultures grown outdoors in Israel is a Gymnodinium sp., a dinoflagellate that feeds on the alga. Comparison of the effects of various environmental conditions, i.e., pH, salinity, and temperature, on Gymnodinium and Porphyridium species revealed that the Gymnodinium sp. has sharp optimum curves, whereas the Porphyridium sp. has a wider range of optimum conditions and is also more resistant to extreme environmental variables. The mode of preying on the alga was observed, and the specificity of the Gymnodinium sp. for the Porphyridium sp. was shown. In addition, Gymnodinium extract was shown to contain enzymatic degrading activity specific to the Porphyridium sp. cell wall polysaccharide. PMID:16348059

Ucko, Michal; Cohen, Ephraim; Gordin, Hillel; Arad, Shoshana (Malis)

1989-01-01

43

Glutamate receptor activation evokes calpain-mediated degradation of Sp3 and Sp4, the prominent Sp-family transcription factors in neurons  

PubMed Central

Sp-family transcription factors (Sp1, Sp3 and Sp4) contain a zinc-finger domain that binds to DNA sequences rich in G-C/T. As assayed by RT-PCR analysis of mRNA, western blot analysis, immunofluorescence, and antibody-dependent “supershift” of DNA-binding assays, the prominent Sp-family factors in cerebral neurons were identified as Sp3 and Sp4. By contrast, glial cells were found to express Sp1 and Sp3. We previously showed that the pattern of G-C/T binding activity of Sp-family factors is rapidly and specifically altered by the calcium influx accompanying activation of glutamate receptors. Here, we demonstrate that Sp-factor activity is also lost after a cerebral ischemia/reperfusion injury in vivo. Consistent with its calcium-dependent nature, we found that glutamate’s effect on Sp-family factors could be blocked by inhibitors of calpains, neutral cysteine proteases activated by calcium. Purified calpain I cleaved Sp3 and Sp4 into products that retained G-C/T-binding activity, consistent with species observed in glutamate-treated neurons. These data provide details of an impact of glutamate-receptor activation on molecular events connected to gene expression. PMID:17316402

Mao, Xianrong; Yang, Shao-Hua; Simpkins, James W.; Barger, Steven W.

2007-01-01

44

Helicotylenchus stylocercus n. sp. and Rotylenchus phaliurus n. sp. (Nematoda: Hoplolaimidae) from Costa Rica.  

PubMed

Two new species of plant-parasitic nematodes from Costa Rica are described. Helicotygenchus styloeercus n. sp., from soil around roots of banana at Coto, is distinguished hy the female tail, which bears a large pillarlike ventral projection. Rotylenchus phaliurus n. sp., from soil artmnd roots of Dioscoroea sp. at Sixaola, differs from R. caudaphasmidius in having the conus equal to or more than half the spear length, and large terminal annules on the female tail. PMID:19300653

Siddiqi, M R; Pinochet, J

1979-10-01

45

Sp(3) structures on 14-dimensional manifolds  

NASA Astrophysics Data System (ADS)

The present article investigates Sp(3) structures on 14-dimensional Riemannian manifolds, a continuation of the recent study of manifolds modeled on rank two symmetric spaces (here: SU(6)/Sp(3)). We derive topological criteria for the existence of such a structure and construct large families of homogeneous examples. As a by-product, we prove a general uniqueness criterion for characteristic connections of G structures and that the notions of biinvariant, canonical, and characteristic connections coincide on Lie groups with biinvariant metric.

Agricola, Ilka; Friedrich, Thomas; Höll, Jos

2013-07-01

46

SP100 system design and technology progress  

Microsoft Academic Search

GE, under contract to the U. S. Department of Energy, is designing the SP-100 Space Reactor Power System and developing the requisite technology to provide power in the range of 10’s to 100’s of kWe for a wide variety of missions. SP-100 technology satisfies the emerging high power requirements that will enable future space missions and is readily adaptable to

Allan T. Josloff; Herbert S. Bailey; Donald N. Matteo

1992-01-01

47

SP100 system design and technology progress  

Microsoft Academic Search

GE, under contract to the U. S. Department of Energy, is designing the SP-100 Space Reactor Power System and developing the requisite technology to provide power in the range of 10's to 100's of kWe for a wide variety of missions. SP-100 technology satisfies the emerging high power requirements that will enable future space missions and is readily adaptable to

Allan T. Josloff; Herbert S. Bailey; Donald N. Matteo

1992-01-01

48

SP100 scaleup to 40 MWe  

Microsoft Academic Search

Human space exploration and utilization will require megawatts of electric power for propulsion and surface applications. SP-100 nuclear technology being developed under the DOE\\/NASA\\/DOD Ground Engineering System contact is directly applicable to these missions. Designs of three scaleup systems delivering 5, 10 and 40 MWe using SP-100 reactor technology in conjunction with both static and dynamic power conversion technologies provide

Douglas W. Newkirk; Samir A. Salamah; Samuel L. Stewart; Philip R. Pluta

1991-01-01

49

Isolation of Bartonella rattimassiliensis sp. nov. and Bartonella phoceensis sp. nov. from European Rattus norvegicus  

PubMed Central

Thirty-three isolates of Bartonella spp., including 11 isolates not belonging to previously known species, were isolated from 66 Rattus norvegicus subjects trapped in the city of Marseille, France. Based on seven different gene sequences, the 11 isolates were assigned to Bartonella rattimassiliensis sp. nov. and Bartonella phoceensis sp. nov. PMID:15297537

Gundi, Vijay A. K. B.; Davoust, Bernard; Khamis, Atieh; Boni, Mickaël; Raoult, Didier; La Scola, Bernard

2004-01-01

50

IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3.  

PubMed

IL-10 is an 18-kDa cytokine with a key role in homeostatic control of inflammatory and immune responses. We have investigated how transcription of the IL-10 gene is regulated, so as to be able to understand the circumstances of IL-10 expression in both health and disease. In the mouse, IL-10 gene expression is regulated by a TATA-type promoter with a critical cis-acting element containing GGA repeats located at -89 to -77. Its complementary sequence is similar to the cis-acting elements (TCC repeats) in the promoters of genes encoding epidermal growth factor receptor and CD58. All these elements comprise a common CCTCCT sequence with less conserved C + T-rich sequences. Eliminating this CCTCCT sequence results in a marked reduction in promoter activity, suggesting a necessary role in IL-10 gene expression. Despite its dissimilarity to the G + C-rich Sp1 consensus sequence (GC box), Sp1 and Sp3 transcription factors could be shown to bind to this motif. The requirement for Sp1 and Sp3 in transcription of IL-10 was confirmed using Drosophila SL2 cells, which lack endogenous Sp factors. These results suggest that the transcription of IL-10 is positively regulated by both Sp1 and Sp3. PMID:10861063

Tone, M; Powell, M J; Tone, Y; Thompson, S A; Waldmann, H

2000-07-01

51

Nodulation in black locust by the Gammaproteobacteria Pseudomonas sp. and the Betaproteobacteria Burkholderia sp.  

PubMed

Nodulation abilities of bacteria in the subclasses Gammaproteobacteria and Betaproteobacteria on black locust (Robinia pseudoacacia) were tested. Pseudomonas sp., Burkholderia sp., Klebsiella sp., and Paenibacillus sp. were isolated from surface-sterilized black locust nodules, but their nodulation ability is unknown. The aims of this study were to determine if these bacteria are symbiotic. The species and genera of the strains were determined by RFLP analysis and DNA sequencing of 16S rRNA gene. Inoculation tests and histological studies revealed that Pseudomonas sp. and Burkholderia sp. formed nodules on black locust and also developed differentiated nodule tissue. Furthermore, a phylogenetic analysis of nodA and a BLASTN analysis of the nodC, nifH, and nifHD genes revealed that these symbiotic genes of Pseudomonas sp. and Burkholderia sp. have high similarities with those of rhizobial species, indicating that the strains acquired the symbiotic genes from rhizobial species in the soil. Therefore, in an actual rhizosphere, bacterial diversity of nodulating legumes may be broader than expected in the Alpha-, Beta-, and Gammaproteobacteria subclasses. The results indicate the importance of horizontal gene transfer for establishing symbiotic interactions in the rhizosphere. PMID:20542651

Shiraishi, Ayami; Matsushita, Norihisa; Hougetsu, Taizo

2010-08-01

52

Effect of adhesion to particles on the survival and activity of Nitrosomonas sp. and Nitrobacter sp  

Microsoft Academic Search

The adhesion of Nitrosomonas sp. and Nitrobacter sp. cells isolated from fishpond sediment to different solid particles was studied. Nitrosomonas and Nitrobacter cells rapidly attached to particles of bentonite, calcium carbonate, amberlite, and fishpond sediment, however they did not adhere to phenyl-sepharose beads. The nitrifying activity of attached bacteria was greater than the activity of freely suspended cells or the

Shaher Diab; Moshe Shilo

1988-01-01

53

Cheylostigmaeus tarae sp. nov. and Stigmaeus delaramae sp. nov. (Acari: Stigmaeidae) from Kurdistan, Iran.  

PubMed

Two new species belonging to the family Stigmaeidae, Cheylostigmaeus tarae sp. nov. and Stigmaeus delaramae sp. nov., are described from specimens collected from soil and litter under pear trees, Pyrus communis L. (Rosaceae) in Iran. A key to all Iranian species of the genera Cheylostigmaeus (male) and Stigmaeus (female) are provided.  PMID:25082045

Khanjani, Mohammad; Nasrollahi, Siamak; Zamani, Ali Sina; Fayaz, Bahman Asali

2014-01-01

54

Sp8 regulates inner ear development  

PubMed Central

A forward genetic screen of N-ethyl-N-nitrosourea mutagenized Xenopus tropicalis has identified an inner ear mutant named eclipse (ecl). Mutants developed enlarged otic vesicles and various defects of otoconia development; they also showed abnormal circular and inverted swimming patterns. Positional cloning identified specificity protein 8 (sp8), which was previously found to regulate limb and brain development. Two different loss-of-function approaches using transcription activator-like effector nucleases and morpholino oligonucleotides confirmed that the ecl mutant phenotype is caused by down-regulation of sp8. Depletion of sp8 resulted in otic dysmorphogenesis, such as uncompartmentalized and enlarged otic vesicles, epithelial dilation with abnormal sensory end organs. When overexpressed, sp8 was sufficient to induce ectopic otic vesicles possessing sensory hair cells, neurofilament innervation in a thickened sensory epithelium, and otoconia, all of which are found in the endogenous otic vesicle. We propose that sp8 is an important factor for initiation and elaboration of inner ear development. PMID:24722637

Chung, Hyeyoung A.; Medina-Ruiz, Sofia; Harland, Richard M.

2014-01-01

55

SP2Bench: A SPARQL Performance Benchmark  

NASA Astrophysics Data System (ADS)

A meaningful analysis and comparison of both existing storage schemes for RDF data and evaluation approaches for SPARQL queries necessitates a comprehensive and universal benchmark platform. We present SP2Bench, a publicly available, language-specific performance benchmark for the SPARQL query language. SP2Bench is settled in the DBLP scenario and comprises a data generator for creating arbitrarily large DBLP-like documents and a set of carefully designed benchmark queries. The generated documents mirror vital key characteristics and social-world distributions encountered in the original DBLP data set, while the queries implement meaningful requests on top of this data, covering a variety of SPARQL operator constellations and RDF access patterns. In this chapter, we discuss requirements and desiderata for SPARQL benchmarks and present the SP2Bench framework, including its data generator, benchmark queries and performance metrics.

Schmidt, Michael; Hornung, Thomas; Meier, Michael; Pinkel, Christoph; Lausen, Georg

56

SP-100 flight qualification testing assessment  

NASA Technical Reports Server (NTRS)

The SP-100 is a compact space power system driven by a nuclear reactor that provides 100 kWe to the user at 200 VDC. The thermal energy generated by the nuclear reactor is converted into electrical energy by passive thermoelectric devices. Various options for tailoring the MIL-STD-1540B guidelines to the SP-100 nuclear power system are discussed. This study aids in selecting the appropriate qualification test program based on the cost, schedule, and test effectiveness of the various options.

Jeanmougin, Nanette M.; Moore, Roger M.; Wait, David L.; Jacox, Michael G.

1988-01-01

57

Rhodoferax ferrireducens sp. nov., a psychrotolerant, facultatively anaerobic bacterium  

E-print Network

Rhodoferax ferrireducens sp. nov., a psychrotolerant, facultatively anaerobic bacterium ferrireducens sp. nov. INTRODUCTION Fe(III) is often an abundant electron acceptor for microbial respiration other forms of respiration. However, it is becoming increasingly apparent that there is a wide

Lovley, Derek

58

Faecal survey of deer for Yersinia pseudotuberculosis and Salmonella sp  

Microsoft Academic Search

The results of a national survey of faeces from clinically normal deer for Salmonella sp. and Yersinia pseudotuberculosis are reported. Five isolates of Y. pseudotuberculosis and none of Salmonella sp. were made from 3810 faeces representing 122 farms.

T. G. Henderson; P. Hemmingsen

1983-01-01

59

MAGNET CELL SAFETY PROCEDURE SP-16 Revision 02  

E-print Network

MAGNET CELL SAFETY PROCEDURE SP-16 Page i Revision 02 July 19, 2006 NATIONAL HIGH MAGNETIC: MAGNET CELL SAFETY PROCEDURE ______________________________________________________ HEAD of MAGNET ______________________________________________________ HEAD of FACILITIES John Kynoch #12;MAGNET CELL SAFETY PROCEDURE SP-16 Pageii Revision 02 July 19

Weston, Ken

60

SP100 initial startup and restart control strategy  

Microsoft Academic Search

Startup control strategies for SP-100 are described. Revised control and operating strategies are discussed which have been developed and tested using the SP-100 dynamic simulation model Aries-GFS (Generic Flight System).

Frank J. Halfen; Kwok K. Wong; Dennis M. Switick; Jaikaran N. Shukla

1992-01-01

61

Power transmission studies for tethered SP-100  

NASA Technical Reports Server (NTRS)

The tether and/or transmission line connecting the SP-100 to space station presents some unorthodox challenges in high voltage engineering, power transmission, and distribution. The line, which doubles as a structural element of this unusual spacecraft, will convey HVDC from SP-100 to the platform in low Earth orbit, and environment where the local plasma is sufficient to cause breakdown of exposed conductors at potentials of only a few hundred volts. Its anticipated several years operation, and continuously accumulating exposure to meteoroids and debris, raises an increasing likelihood that mechanical damage, including perforation, will be sustained in service. The present concept employs an array of gas insulated solid wall aluminum coaxial tubes; a conceptual design which showed basic feasibility of the SP-100 powered space station. Practical considerations of launch, deployment and assembly have lead to investigation of reel deployable, dielectric insulated coaxial cables. To be competitive, the dielectric would have to operate reliably in a radiation environment under electrical stresses exceeding 50 kV/cm. The SP-100 transmission line high voltage interfaces are also considered.

Bents, David J.

1988-01-01

62

Power transmission studies for tethered SP-100  

NASA Technical Reports Server (NTRS)

The tether and/or transmission line connecting the SP-100 to Space Station presents some unorthodox challenges in high voltage engineering, power transmission, and distribution. The line, which doubles as a structural element of this unusual spacecraft, will convey HVDC from SP-100 to the platform in low Earth orbit, and environment where the local plasma is sufficient to cause breakdown of exposed conductors at potentials of only a few hundred volts. Its anticipated several years operation, and continuously accumulating exposure to meteoroids and debris, raises an increasing likelihood that mechanical damage, including perforation, will be sustained in service. The present concept employs an array of gas insulated solid wall aluminum coaxial tubes; a conceptual design which showed basic feasibility of the SP-100 powered Space Station. Practical considerations of launch, deployment and assembly have led to investigation of reel deployable, dielectric insulated coaxial cables. To be competitive, the dielectric would have to operate reliably in a radiation environment under electrical stresses exceeding 50 kV/cm. The SP-100 transmission line high voltage interfaces are also considered.

Bents, David J.

1988-01-01

63

Extended SP-100 reactor power systems capability  

NASA Technical Reports Server (NTRS)

The SP-100 space nuclear power system development program and the NASA Civil Space Technology Initiative (CSTI) are discussed. The advanced technologies being developed for the CSTI high capacity nuclear reactor power system are outlined. The relationship between the CSTI and the Pathfinder project is considered.

Bloomfield, H. S.; Winter, J. M.; Mckissock, B. I.; Sovie, R. J.

1988-01-01

64

Hepatozoon sp. in wild carnivores in Texas.  

PubMed

Twelve coyotes (Canis latrans), three bobcats (Lynx rufus) and six ocelots (Felis pardalis) from the Gulf Coast of Texas were infected with Hepatozoon sp. The geographic distribution of infected wild animals coincides with the highest prevalence of Hepatozoon canis infection in domestic dogs for which the wild species may act as a reservoir. PMID:3411720

Mercer, S H; Jones, L P; Rappole, J H; Twedt, D; Lack, L L; Craig, T M

1988-07-01

65

[Sulfation of naringenin by Mucor sp].  

PubMed

Naringenin (1) was transformed to three metabolites (2-4) by Mucor sp. Based on LCMS(n)-IT-TOF and NMR spectroscopic data, 2-4 were identified as naringenin-7-O-sulphate, naringenin-4'-O-sulphate, and naringenin-5-O-sulphate, respectively. These results might provide hints to the mammalian/human metabolism of naringenin. PMID:25272838

Ruan, Fei-Ying; Chen, Ri-Dao; Li, Jian-Hua; Zhang, Min; Xie, Ke-Bo; Wang, Yan; Feng, Ru; Dai, Jun-Gui

2014-06-01

66

Sleep in the tortoise Kinosternon sp  

Microsoft Academic Search

Summary Individuals ofKinosternon sp., previously confined to laboratory conditions, were chronically implanted with electrodes for electroencephalogram, electro-oculogram and electrocardiogram recording. Behavioral states of waking and sleep were clearly observed. Two sleep stages were present: quiet sleep and REM or active sleep. Electrical cerebral activity was polymorphic and irregular. EEG frequencies declined and amplitudes diminished with sleep. Arrhythmic spikes occurred during

F. Ayala-Guerrero; M. Yoshida; N. Karasawa; M. Teramura; H. Ueda; I. Nagatsu

1987-01-01

67

Early SP100 flight mission designs  

Microsoft Academic Search

Early flight mission objectives can be met with a Space Reactor Power System (SRPS) using thermoelectric conversion in conjunction with fast spectrum, lithium-cooled reactors. This paper describes two system design options using thermoelectric technology to accommodate an early launch. In the first of these options, radiatively coupled Radioiosotope Thermoelectric Generator (RTG) unicouples are adapted for use with a SP-100-type reactor

Allan T. Josloff; Neal F. Shepard; Aaron S. Kirpich; Ronald Murata; Michael A. Smith; James D. Stephen

1993-01-01

68

SP100 reactor and shield design update  

Microsoft Academic Search

The SP-100 technical specification for the generic flight system has been updated to eliminate design specific and unnecessarily restrictive requirements. Relaxation of these requirements allows considerably more flexibility to the reactor and shield designer to minimize the mass of the system. Ground Engineering System (GES) Program test results are also beginning to become available to the designer. Some of these

Nelson A. Deane; Samuel L. Stewart; Thomas F. Marcille; Douglas W. Newkirk

1992-01-01

69

SP100 control drive assembly development  

Microsoft Academic Search

The SP-100 is an electrical generating nuclear power system for space operation. This paper describes the nuclear reactor control systems and the methods used to assure reliable performance for the 10-year design life. Reliable performance is achieved by redundancy and by selecting highly reliable components and design features. Reliability is quantified by analysis using established reliability data. Areas lacking reliability

Thomas Gleason; A. Richard Gilchrist; Gary Schuster

1993-01-01

70

Uranium accumulation by Pseudomonas sp. EPS5028  

Microsoft Academic Search

Pseudomonas sp. EPS-5028 was examined for the ability to accumulate uranium from solutions. The uptake of uranium by this microorganism is very rapid and is affected by pH but not by temperature, metabolic inhibitors, culture time and the presence of various cations and anions. The amount of uranium absorbed by the cells increased as the uranium concentration of the solution

Ana M. Marqués; Xavier Roca; M. Dolores Simon-Pujol; M. Carmen Fuste; Francisco Congregado

1991-01-01

71

Remote sensing data of SP mountain and SP lava flow in north-central Arizona  

NASA Technical Reports Server (NTRS)

Multifrequency airborne radar image data of SP Mountain and SP flow in north-central Arizona were obtained in diverse viewing directions and direct and cross-polarization and compared with surface and aerial photography, Landsat multispectral scanner data, airborne thermal infrared imagery, surface geology, and surface roughness statistics. The extremely blocky, basaltic andesite of SP flow is brighter on direct-polarization K-band images than on cross-polarized images taken simultaneously. This effect is explained by multiple scattering and the strong wavelength dependence of polarization effects caused by the rectilinear basaltic andesite scatters. Two distinct types of surface relief on SP flow, one extremely blocky, the other subdued, are clearly discriminated on the visible and thermal wavelength images but are separable only on the longer wavelength L-band radar image data.

Schaber, G. G.; Elachi, C.; Farr, T. G.

1980-01-01

72

Targeting Sp1 transcription factors in prostate cancer therapy.  

PubMed

Transcription factors are proteins that regulate gene expression by binding to specific DNA sequences within gene promoter regions. Specificity protein (Sp) family transcription factors play a critical role in various cellular processes and have been shown to be associated with tumorigenesis. The Sp family consists of several members that contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus and serine/threonine- and glutamine-rich transactivation domains at the N-terminal. Sp1 is elevated in several cancers including prostate and is associated with the prognosis of patients. Sp1, Sp3, and Sp4 regulate a variety of cancer associated genes that are involved in cell cycle, proliferation, cell differentiation, and apoptosis. Studies have shown that in prostate cancer, Sp1 regulates important genes like androgen receptor, TGF-?, c-Met, fatty acid synthase, matrix metalloprotein (MT1-MMP), PSA, and ?-integrin. These results highlight the importance of Sp1 in prostate cancer and emphasize the potential therapeutic value of targeting Sp1. Several strategies, including the use of natural and synthetic compounds, have been used to inhibit Sp1 in prostate cancer. These include polyphenol quercetin, betulinic acid, acetyl-11-keto-beta-boswellic acid, tea phenols, isothiocyanates, thiazolidinediones, arsenic trioxide, and selenium. This review will describe the association of Sp proteins in prostate cancer with a special emphasis on some of the agents tested to target Sp proteins for the treatment of this malignancy. PMID:22022994

Sankpal, Umesh T; Goodison, Steven; Abdelrahim, Maen; Basha, Riyaz

2011-09-01

73

Iron-catalyzed decarboxylative cross coupling reactions and palladium-catalyzed sp2-sp3 coupling of coumarins.  

E-print Network

, the same concept can be applied to the sp2?sp3 couplings of pyrones and thiocoumarins. A variety of biologically important heteroaromatics can be readily functionalized without the need for strong bases or stoichiometric organometallics...

Trivedi, Rushi

2009-12-15

74

Coccidioides posadasii infection alters the expression of pulmonary surfactant proteins (SP)-A and SP-D  

PubMed Central

Background Coccidioidomycosis or Valley Fever is caused by Coccidioides in Southwest US and Central America. Primary pulmonary infection is initiated by inhalation of air-borne arthroconidia. Since, lung is the first organ that encounters arthroconidia, different components of the pulmonary innate immune system may be involved in the regulation of host defense. Pulmonary surfactant proteins (SP)-A and SP-D have been recognized to play an important role in binding and phagocytosis of various microorganisms, but their roles in Coccidioides infection are not known. Methods In this study, we studied the changes in amounts of pulmonary SP-A, SP-D and phospholipid in murine model of Coccidioides posadasii infection, and binding of SP-A and SP-D to Coccidioidal antigens. Mice were challenged intranasally with a lethal dose of C. posadasii (n = 30 arthroconidia) and bronchoalveolar lavage fluid (BALF) samples were collected on day 10, post infection. In another group of animals, mice were immunized with protective formalin killed spherule (FKS) vaccine prior to infection. The concentrations of BALF SP-A, SP-D, total phospholipid were measured using enzyme linked immunosorbent assay and biochemical assays. Results We found that in lavage fluid samples of C. posadasii infected mice, the concentrations of total phospholipid, SP-A and SP-D were 17 % (SEM 3.5, p < 0.001), 38 % (SEM 5.8, p < 0.001) and 4 % (SEM 1.3, p < 0.001) of those in lavage fluid samples of non-infected control mice, respectively. However, the concentrations of SP-A and SP-D remained unchanged in BALF samples of C. posadasii protected mice after immunization with FKS vaccine. Also, we found that both SP-A and SP-D bind to Coccidiodal antigens. Conclusion Our results suggest that the C. posadasii infection perturbs the pulmonary SP-A, SP-D, and phospholipids, potentially enabling the disease progression and promoting fungal dissemination. PMID:15588319

Awasthi, Shanjana; Magee, D Mitchell; Coalson, Jacqueline J

2004-01-01

75

Effects of Lung Surfactant Proteins, SP-B and SP-C, and Palmitic Acid on Monolayer Stability  

E-print Network

Effects of Lung Surfactant Proteins, SP-B and SP-C, and Palmitic Acid on Monolayer Stability Junqi and fluorescence and atomic force microscopy images of synthetic model lung surfactants were used to determine-C on the morphology and function of surfactant monolayers. Lung surfactant-specific protein SP-C and peptides based

Zasadzinski, Joseph A.

76

Candida asparagi sp. nov., Candida diospyri sp. nov. and Candida qinlingensis sp. nov., novel anamorphic, ascomycetous yeast species.  

PubMed

Among ascomycetous yeasts that were isolated from several nature reserve areas in China, three anamorphic strains isolated from soil (QL 5-5T) and fruit (QL 21-2T and SN 15-1T) were revealed, by conventional characterization and molecular phylogenetic analysis based on internal transcribed spacer and large subunit (26S) rRNA gene D1/D2 region sequencing, to represent three novel species in the genus Candida. Candida qinlingensis sp. nov. (type strain, QL 5-5T=AS 2.2524T=CBS 9768T) was related closely to a teleomorphic species, Williopsis pratensis. The close relatives of Candida diospyri sp. nov. (type strain, QL 21-2T=AS 2.2525T=CBS 9769T) are Candida friedrichii and Candida membranifaciens. Candida asparagi sp. nov. (type strain, SN 15-1T=AS 2.2526T=CBS 9770T) forms a clade with Candida fructus. PMID:15280322

Lu, Hui-Zhong; Jia, Jian-Hua; Wang, Qi-Ming; Bai, Feng-Yan

2004-07-01

77

Significant biological role of Sp1 transactivation in multiple myeloma  

PubMed Central

Purpose The transcription factor Sp1 controls number of cellular processes by regulating the expression of critical cell cycle, differentiation and apoptosis-related genes containing proximal GC/GT-rich promoter elements. We here provide both experimental and clinical evidence that Sp1 plays an important regulatory role in MM cell growth and survival. Experimental design We have investigated the functional Sp1 activity in MM cells using a plasmid with renilla luciferase reporter gene driven by Sp1-responsive promoter. We have also used both SiRNA and ShRNA-mediated Sp1 knock-down to investigate the growth and survival effects of Sp1 on MM cells, and further investigated the anti-MM activity of Terameprocol (TMP), a small molecule which specifically competes with Sp1-DNA binding in vitro and in vivo. Results We have confirmed high Sp1 activity in MM cells which is further induced by adhesion to bone marrow stromal cells (BMSC). Sp1 knock down decreases MM cell proliferation and induces apoptosis. Sp1-DNA binding inhibition by TMP inhibits MM cell growth both in vitro and in vivo, inducing caspase 9-dependent apoptosis and overcoming the protective effects of BMSCs. Conclusions Our results demonstrate Sp1 as an important transcription factor in myeloma that can be therapeutically targeted for clinical application by TMP. PMID:21856768

Fulciniti, Mariateresa; Amin, Samir; Nanjappa, Puru; Rodig, Scott; Prabhala, Rao; Li, Cheng; Minvielle, Stephane; Tai, Yu-tzu; Tassone, Pierfrancesco; Avet-Loiseau, Herve; Hideshima, Teru; Anderson, Kenneth C.; Munshi, Nikhil C.

2015-01-01

78

Sp6 and Sp8 transcription factors control AER formation and dorsal-ventral patterning in limb development.  

PubMed

The formation and maintenance of the apical ectodermal ridge (AER) is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6-/-;Sp8+/-) exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/?catenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning. PMID:25166858

Haro, Endika; Delgado, Irene; Junco, Marisa; Yamada, Yoshihiko; Mansouri, Ahmed; Oberg, Kerby C; Ros, Marian A

2014-08-01

79

A pumilio homolog in Polycelis sp.  

PubMed

Pumilio proteins (PUMs), members of the pumilio/fem-3 mRNA-binding factor (PUF) family, are eukaryote-specific RNA-binding proteins. We isolated a 2,048-basepair cDNA fragment of a pumilio homolog from the planarian flatworm Polycelis sp. This pumilio protein (PyPUM) contains a conserved pumilio homology domain (PUM-HD) consisting of eight repeats and two flanking half repeats. PyPUM shows high similarity to Dugesia japonica pumilio (DjPUM) from another planarian D. japonica, and their PUM-HD also shows high similarity to each other. Furthermore, our data showed that there is a flatworm-specific spacer between repeats 7 and 8. Phylogenetic analysis showed that PyPUM has a closer relationship to other PUM homologs from flatworms. These results provide a foundation for future functional studies of pumilio gene in Polycelis sp. PMID:24292205

Yuwen, Yanqing; Dong, Zimei; Si, Xiaohui; Chen, Guangwen

2014-02-01

80

One-dimensional s-p superlattice  

E-print Network

The physics of one dimensional optical superlattices with resonant $s$-$p$ orbitals is reexamined in the language of appropriate Wannier functions. It is shown that details of the tight binding model realized in different optical potentials crucially depend on the proper determination of Wannier functions. We discuss the properties of a superlattice model which quasi resonantly couples $s$ and $p$ orbitals and show its relation with different tight binding models used in other works.

Wojciech Ganczarek; Michele Modugno; Giulio Pettini; Jakub Zakrzewski

2014-06-23

81

Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov.  

PubMed

Sixty strains of Gram-negative, anaerobic, rod-shaped bacteria from human sources initially assigned to Leptotrichia buccalis (n=58) and 'Leptotrichia pseudobuccalis' (n=2) have been subjected to polyphasic taxonomy. Full-length 16S rDNA sequencing, DNA-DNA hybridization, RAPD, SDS-PAGE of whole-cell proteins, cellular fatty acid analysis and enzymic/biochemical tests supported the establishment of four novel Leptotrichia species from this collection, Leptotrichia goodfellowii sp. nov. (type strain LB 57(T)=CCUG 32286(T)=CIP 107915(T)), Leptotrichia hofstadii sp. nov. (type strain LB 23(T)=CCUG 47504(T)=CIP 107917(T)), Leptotrichia shahii sp. nov. (type strain LB 37(T)=CCUG 47503(T)=CIP 107916(T)) and Leptotrichia wadei sp. nov. (type strain LB 16(T)=CCUG 47505(T)=CIP 107918(T)). Light and electron microscopy showed that the four novel species were Gram-negative, non-spore-forming and non-motile rods. L. goodfellowii produced arginine dihydrolase, beta-galactosidase, N-acetyl-beta-glucosaminidase, arginine arylamidase, leucine arylamidase and histidine arylamidase. L. shahii produced alpha-arabinosidase. L. buccalis and L. goodfellowii fermented mannose and were beta-galactosidase-6-phosphate positive. L. goodfellowii, L. hofstadii and L. wadei were beta-haemolytic. L. buccalis fermented raffinose. With L. buccalis, L. goodfellowii showed 3.8-5.5 % DNA-DNA relatedness, L. shahii showed 24.5-34.1 % relatedness, L. hofstadii showed 27.3-36.3 % relatedness and L. wadei showed 24.1-35.9 % relatedness. 16S rDNA sequencing demonstrated that L. hofstadii, L. shahii, L. wadei and L. goodfellowii each formed individual clusters with 97, 96, 94 and 92 % similarity, respectively, to L. buccalis. PMID:15023979

Eribe, Emenike R K; Paster, Bruce J; Caugant, Dominique A; Dewhirst, Floyd E; Stromberg, Verlyn K; Lacy, George H; Olsen, Ingar

2004-03-01

82

New phomopsolides from a Penicillium sp.  

PubMed

Investigation of the bioactive compounds from a Penicillium sp. isolated from the inner bark of the Pacific yew, Taxus brevifolia, led to the isolation of the known furanone 1, and a series of phomopsolides. The phomopsolide fractions contained phomopsolides A and B, which have previously been described, and three new phomopsolides. The structures of the new phomopsolides were deduced by comparison of their NMR spectra to those of the known compounds. PMID:9392888

Stierle, D B; Stierle, A A; Ganser, B

1997-11-01

83

Eimeria clethrionomysis sp. n., Eimeria gallatii sp. n., Eimeria pileata sp. n. and Eimeria marconii sp. n. from the red-backed vole Clethrionomys gapperi Vigors, from Pennsylvania.  

PubMed

Four new eimerian species are described from red-backed voles, Clethrionomys gapperi in Pennsylvania. Sporulated oocysts of Eimeria clethrionomyis sp. n. are ellipsoidal, 18.8 (16.5-21.5) x 14.9 (14.0-16.5) with elongate, ovoid sporocysts, 10.6 (9.5-12.0) x6.1 (5.5-7.0). The oocyst wall is smooth, with 2 layers, and thins, with terminal cap at one or both ends. Polar granules, dark Stieda body and sporocyst residuum are present. The oocyst residuum is absent. Sporulated oocysts of Eimeria gallatii sp. n. are ellipsoidal, 27.7 (21-32) x 19.3 (17-24) with ovoid sporocysts, 13.5 (12-15) x 8.8 (8-10). The oocyst wall is smooth, 2-layered, with a micropyle and thin wall at the end opposite the micropyle. Polar granules, Stieda body and sporocyst residuum are present. The oocyst residuum is atypical, of cobwebby material. Sporulated oocysts of Eimeria pileata sp. n. are subspherical to spherical, 25.2 (20.5-29.5) x 22.5 (19.5-25.5) with ellipsoidal sporocysts, 13.4(10.5-15.0) x 8.4 (7.5-9.5). The oocyst wall is rough, pitted, striated, 2-layered, with no micropyle. Polar granules, oocyst and sporocyst residuum, Stieda body and stiedal cap are present. Sporulated oocysts of Eimeria marconii sp. n. are ellipsoidal, 13.0 (10.5-15-0) x 10.6 (9.5-12.0) with elongate, ovoid sporocysts, 7.7 (7.0-8.5) x 4.2 (3.0-4.5). The oocyst wall is smooth, single-layered, with no micropyle. Polar granules, dark Stiedal body and sporocyst residuum are present. There is no oocyst residuum. PMID:397341

Straneva, J E; Kelley, G L

1979-11-01

84

SP-100 design, safety, and testing  

NASA Technical Reports Server (NTRS)

The SP-100 Program is developing a nuclear reactor power system that can enhance and/or enable future civilian and military space missions. The program is directed to develop space reactor technology to provide electrical power in the range of tens to hundreds of kilowatts. The major nuclear assembly test is to be conducted at the Hanford Site near Richland, Washington, and is designed to validate the performance of the 2.4-MWt nuclear and heat transport assembly.

Cox, Carl. M.; Mahaffey, Michael M.; Smith, Gary L.

1991-01-01

85

Introducing secure peergroups in SP2A  

Microsoft Academic Search

Service-oriented grids are aiming at new applications beyond traditional resource-oriented scientific computing. We argue that Community Support is emerging as the real killer application. Service-oriented systems provide a flexible solution for work groups which belong to university campuses, research labs, enterprises, finance environments. We are building the service-oriented P2P architecture (SP2A), which is the result of the positive convergence between

Michele Amoretti; Matteo Bisi; Francesco Zanichelli; Gianni Conte

2005-01-01

86

New tricycloalternarenes from fungus Alternaria sp.  

PubMed

Two new tricycloalternarenes I (1) and J (2), together with five known derivatives (3-7), were isolated from the culture of marine fungus Alternaria sp. The structures were elucidated by a combination of spectroscopic approach ((1)H, (13)C NMR, HMBC, COSY, and NOESY) and the low-temperature (100 K) single-crystal X-ray crystallography analysis. The antimicrobial assays of tricycloalternarenes I (1) and J (2) were tested. PMID:25402226

Shi, Xiu; Wei, Wei; Zhang, Wen-Jing; Hua, Cheng-Pin; Chen, Chao-Jun; Ge, Hui-Ming; Tan, Ren-Xiang; Jiao, Rui-Hua

2015-02-01

87

Candida alocasiicola sp. nov., Candida hainanensis sp. nov., Candida heveicola sp. nov. and Candida musiphila sp. nov., novel anamorphic, ascomycetous yeast species isolated from plants.  

PubMed

In a taxonomic study on the ascomycetous yeasts isolated from plant materials collected in tropical forests in Yunnan and Hainan Provinces, southern China, four strains isolated from tree sap (YJ2E(T)) and flowers (YF9E(T), YWZH3C(T) and YYF2A(T)) were revealed to represent four undescribed yeast species. Molecular phylogenetic analysis based on the large subunit (26S) rRNA gene D1/D2 domain sequences showed that strain YJ2E(T) was located in a clade together with Candida haemulonii and C. pseudohaemulonii. Strain YF9E(T) was most closely related to C. azyma and strain YWZH3C(T) to C. sorbophila and C. spandovensis. Strain YYF2A(T) was clustered in a clade containing small-spored Metschnikowia species and related anamorphic Candida species. The new strains differed from their closely related described species by more than 10% mismatches in the D1/D2 domain. No sexual states were observed for the four strains on various sporulation media. The new species are therefore assigned to the genus Candida and described as Candida alocasiicola sp. nov. (type strain, YF9E(T) = AS 2.3484(T) = CBS 10702(T)), Candida hainanensis sp. nov. (type strain, YYF2A(T) = AS 2.3478(T) = CBS 10696(T)), Candida heveicola sp. nov. (type strain, YJ2E(T) = AS 2.3483(T) = CBS 10701(T)) and Candida musiphila sp. nov. (type strain, YWZH3C(T) = AS 2.3479(T) = CBS 10697(T)). PMID:18344072

Wang, Shi-An; Jia, Jian-Hua; Bai, Feng-Yan

2008-08-01

88

SP100 Heat Source Heat Exchanger Design  

Microsoft Academic Search

A conceptual design for a liquid metal to gas counterflow heat exchanger has been developed for use in the SP-100 Closed Brayton Cycle (CBC) Space Reactor Power System (SRPS). The Heat Source Heat Exchanger (HSHX) is required to transfer 80 kWt from the 1350 K lithium reactor coolant to the He\\/Xe working fluid of the Brayton Rotating Unit (BRU). Trade

T. Ted Fallas; Andrew W. Desepte; Robert J. Hill; Georgi B. Manjarrez; Enrique R. Solorzano; Samir A. Salamah; Raphael Yahalom

1994-01-01

89

SP100 thermoelectric converter technology development  

Microsoft Academic Search

The SP-100 thermoelectric power converter utilizes reactor-delivered heat and transforms it into usable electric power by purely static means. The converter features conduction coupling of TE cells to both the heat source and the heat rejection elements. The conduction coupling technique offers significant improvement in the system specific power since it avoids the losses associated with parasitic Delta(T)s across the

D. N. Matteo; J. A. Bond; R. J. Rosko

1992-01-01

90

Analysis of SP100 critical experiments  

Microsoft Academic Search

In support of the SP-100 space nuclear power source program, preliminary critical benchmark experiments were performed at the ZPPR facility at ANL-W. These configurations are representative of small, fast-spectrum, BeO-reflected, liquid metal-cooled space reactor designs at a 300-kWe power level. Analyses were performed using MCNP (Monte Carlo) and TWODANT (discrete ordinates) transport codes to calculate system criticality, control worth, and

J. L. Sapir; D. I. Brandon; P. J. Collins; C. L. Cowan; C. A. Porter; S. V. Andre

1988-01-01

91

SP100 status and technology accomplishments  

Microsoft Academic Search

There are many space missions that require electric power in the 10-kW(electric) to 1-MW(electric) range. The SP-100 program will provide technology that demonstrates that a nuclear reactor heat source and passive thermoelectric conversion can reliably provide electrical power over this full range for 10 yr. Based on the generic flight system (GFS) design at 100 kW(electric), tests will be conducted

J. S. Armijo; A. T. Josloff; D. N. Matteo; H. S. Bailey; P. R. Pluta

1989-01-01

92

Pasteuria sp. Parasitizing Trophonema okamotoi in Florida.  

PubMed

Two populations of Trophonema okamotoi parasitized by Pasteuria sp. were found on Liquidambar styraciflua (sweetgum) and on an unidentified tropical grass in north-central Florida. Endospores of this Pasteuria sp. attached to motile vermiform second-stage juveniles (J2) and males of T. okamotoi, but not to other developmental stages. Sporangia and new endospores were produced only inside the bodies of swollen and sedentary third- and fourth-stage juveniles and females that developed in the host roots. No egg masses were produced by infected T. okamotoi females. The endospore diameter from the tropical grass population was 4.93 mum and the central core diameter was 1.97 mum; measurements of endospores from the sweetgum populations were similar. Endospores that were collected from T. okamotoi and added to uninfected T. okamotoi and other plant-parasitic nematodes attached/to J2 of T. okamotoi but did not attach to juveniles and adults of Helicotylenchus pseudorotrustus, Pratylenchus brachyurus, or to J2 of either Meloidogyne arenaria race 1, M. incognita race 1, M. javanica, or Tylenchulus semipenetrans. Pasteuria sp. from T. okamotoi differed from the described Pasteuria species in endospore size, host preference, and rate of attachment. PMID:19283199

Inserra, R N; Oostendorp, M; Dickson, D W

1992-03-01

93

Syrian hamsters (Mesocricetus auratus) with simultaneous intestinal Giardia sp., Spironucleus sp., and trichomonad infections.  

PubMed

A commercial facility producing hamsters with a history of infection by dwarf tapeworm (Hymenolepis nana) submitted 15 animals for necropsy and postmortem parasitological and microscopic examination. No tapeworms were detected grossly or microscopically. Fecal examination including gastrointestinal mucosal smears demonstrated mixed intestinal bacteria and low numbers of Giardia sp. Histologic examination of small intestine demonstrated filling of the small intestinal crypts by large numbers of 7-9 µm × 3 µm, rod to crescent or teardrop-shaped flagellates consistent with Spironucleus sp. These organisms had two 1-µm, basophilic, oval nuclei and multiple superficial flagella-like structures. Much larger 10-15 µm × 8-10 µm, oval to pear-shaped organisms were also present in lower numbers and usually located with the crypts. These larger flagellates had multiple flagella and a basophilic rod-shaped nucleus. The larger flagellates included Giardia sp., which had an intimate interface with the surface of the mucosal epithelium, bilaterally symmetry, and binucleation. Lower numbers of trichomonads were also present and were distinguished by an undulating surface membrane and a single nucleus. The mucosa was hyperplastic and moderately inflamed. Although the tapeworm infection was resolved, diagnosis of multiple intestinal flagellates by fecal examination is complicated by the varying sensitivity and diagnostic accuracy of different types of fecal analysis for different flagellate types. Key differences in the morphology and location of the different types of flagellates as observed by histology of intestinal tissues provide important additional diagnostic information to distinguish trichomonads, Spironucleus sp., and Giardia sp. PMID:24081933

Sheppard, Barbara J; Stockdale Walden, Heather D; Kondo, Hirotaka

2013-11-01

94

Electrical properties of magnetron sputtered amorphous carbon films with sequential sp3-rich/sp2-rich layered structure  

NASA Astrophysics Data System (ADS)

The electrical properties of thick amorphous carbon (a-C) films with sequential sp3-rich/sp2-rich layered structure, grown by magnetron sputtering on Si substrates at room temperature, were investigated. At low electric fields, the conduction is due to the variable range hopping mechanism. At high electric fields, thermally assisted band-to-band indirect tunneling is the dominant conduction mechanism, while the Arrhenius plots of the current show a deviation from straight lines in the form of continuous bending satisfying the Meyer-Nelder rule. Comparative studies of low-frequency noise in sp2-rich single layer and sp3-rich/sp2-rich layered a-C films indicate that the noise in the a-C layered originates from traps located mainly at the interfaces of the sp3-rich/sp2-rich bilayers.

Hastas, N. A.; Dimitriadis, C. A.; Tassis, D. H.; Panayiotatos, Y.; Logothetidis, S.; Papadimitriou, D.

2001-11-01

95

Transcription Factor Sp3 Knockout Mice Display Serious Cardiac Malformations?  

PubMed Central

Mice lacking the zinc finger transcription factor specificity protein 3 (Sp3) die prenatally in the C57BL/6 background. To elucidate the cause of mortality we analyzed the potential role of Sp3 in embryonic heart development. Sp3 null hearts display defective looping at embryonic day 10.5 (E10.5), and at E14.5 the Sp3 null mutants have developed a range of severe cardiac malformations. In an attempt to position Sp3 in the cardiac developmental hierarchy, we analyzed the expression patterns of >15 marker genes in Sp3 null hearts. Expression of cardiac ankyrin repeat protein (Carp) was downregulated prematurely after E12.5, while expression of the other marker genes was not affected. Chromatin immunoprecipitation analysis revealed that Sp3 is bound to the Carp promoter region in vivo. Microarray analysis indicates that small-molecule metabolism and cell-cell interactions are the most significantly affected biological processes in E12.5 Sp3 null myocardium. Since the epicardium showed distension from the myocardium, we studied expression of Wt1, a marker for epicardial cells. Wt1 expression was diminished in epicardium-derived cells in the myocardium of Sp3 null hearts. We conclude that Sp3 is required for normal cardiac development and suggest that it has a crucial role in myocardial differentiation. PMID:17923686

van Loo, Pieter Fokko; Mahtab, Edris A. F.; Wisse, Lambertus J.; Hou, Jun; Grosveld, Frank; Suske, Guntram; Philipsen, Sjaak; Gittenberger-de Groot, Adriana C.

2007-01-01

96

Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp.  

PubMed

Environments contaminated with heavy metals negatively impact the living organisms. Ectomycorrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L(-1) in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L(-1) in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated for 30 days at 28 °C. UFSC-Pt116 showed high copper-resistance such as accessed by CL50 determinations (concentration to reduce 50% of the growth) as while as UFSC-PT24 displayed copper-resistance mechanism at 0.50 mmol L(-1) in solid medium. The UFSC-PT24 and UFSC-Sc124 isolates have increased copper-resistance in liquid medium. The higher production of extracellular pigment was detected in UFSC-Pt116 cultures. The UFSC-Pt116 and UFSC-PT24 isolates showed higher resistance for copper and produced higher mycelium biomass than the other isolates. In this way, the isolates UFSG-Pt116 and UFSC-PT24 can be important candidates to survive in copper-contaminated areas, and can show important role in plants symbiosis in these contaminated sites. PMID:24294261

Silva, R F; Lupatini, M; Trindade, L; Antoniolli, Z I; Steffen, R B; Andreazza, R

2013-01-01

97

Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp.  

PubMed Central

Environments contaminated with heavy metals negatively impact the living organisms. Ectomycorrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L?1 in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L?1 in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated for 30 days at 28 °C. UFSC-Pt116 showed high copper-resistance such as accessed by CL50 determinations (concentration to reduce 50% of the growth) as while as UFSC-PT24 displayed copper-resistance mechanism at 0.50 mmol L?1 in solid medium. The UFSC-PT24 and UFSC-Sc124 isolates have increased copper-resistance in liquid medium. The higher production of extracellular pigment was detected in UFSC-Pt116 cultures. The UFSC-Pt116 and UFSC-PT24 isolates showed higher resistance for copper and produced higher mycelium biomass than the other isolates. In this way, the isolates UFSG-Pt116 and UFSC-PT24 can be important candidates to survive in copper-contaminated areas, and can show important role in plants symbiosis in these contaminated sites. PMID:24294261

Silva, R.F.; Lupatini, M.; Trindade, L.; Antoniolli, Z.I.; Steffen, R.B.; Andreazza, R

2013-01-01

98

Sulfitobacter geojensis sp. nov., Sulfitobacter noctilucae sp. nov., and Sulfitobacter noctilucicola sp. nov., isolated from coastal seawater.  

PubMed

Four Gram-stain-negative, aerobic, rod-shaped bacterial strains, MM-124, MM-126, NB-68 and NB-77, were isolated from the coastal seawater or a region with a bloom of sea sparkle around Geoje island in Korea. The sequence similarity values of the 16S rRNA gene between the isolates and Sulfitobacter mediterraneus DSM 12244(T) ranged from 97.7 to 98.2%, and phylogenetic relationships suggested that they belong to a phylogenetic branch that includes the genera Sulfitobacter and Roseobacter. The isoprenoid quinone of all three novel strains was ubiquinone-10 and the major fatty acid was cis-vaccenic acid, as in other species of the genus Sulfitobacter. However, there were several differences in the morphological, physiological and biochemical characteristics among the four strains and the reference species of the genus Sulfitobacter. Moreover, the average nucleotide identity values between the three sequenced isolates and the reference strains were below 76.33, indicating that genomic variation exists between the isolates and reference strains. Chemotaxonomic characteristics together with phylogenetic affiliations and genomic distances illustrate that strains MM-124, NB-68 and NB-77 represent novel species of the genus Sulfitobacter, for which the names Sulfitobacter geojensis sp. nov. (type strain MM-124(T)?=KCTC 32124(T)?=JCM 18835(T)), Sulfitobacter noctilucae sp. nov. (type strain NB-68(T)?=KCTC 32122(T)?=JCM 18833(T)) and Sulfitobacter noctilucicola sp. nov. (type strain NB-77(T)?=KCTC 32123(T)?=JCM 18834(T)) are proposed. PMID:25122614

Kwak, Min-Jung; Lee, Jung-Sook; Lee, Keun Chul; Kim, Kwang Kyu; Eom, Mi Kyung; Kim, Byung Kwon; Kim, Jihyun F

2014-11-01

99

Transfer of Actinomadura spadix Nonomura and Ohara 1971 to Actinoallomurus spadix gen. nov., comb. nov., and description of Actinoallomurus amamiensis sp. nov., Actinoallomurus caesius sp. nov., Actinoallomurus coprocola sp. nov., Actinoallomurus fulvus sp. nov., Actinoallomurus iriomotensis sp. nov., Actinoallomurus luridus sp. nov., Actinoallomurus purpureus sp. nov. and Actinoallomurus yoronensis sp. nov.  

PubMed

Ten actinomycete strains that form chains of spiral or looped spores were isolated from soil and dung samples in Japan. They contained D- and L-lysine, meso-diaminopimelic acid (A2pm), D-glutamic acid and D- and L-alanine in the cell-wall peptidoglycan, madurose as a characteristic whole-cell sugar, MK-9(H6) and MK-9(H8) as the major isoprenoid quinones and iso-C16:0 as the major cellular fatty acid and showed genomic DNA G+C contents of 69-74 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolated actinomycete strains consistently formed a monophyletic cluster with Actinomadura spadix NBRC 14099T and a separate line of descent in the phylogenetic cluster of the family Thermomonosporaceae. Actinomadura spadix NBRC 14099T also contained D- and L-lysine in addition to meso-A2pm. This genetic and phenotypic evidence revealed that the actinomycete strains could be clearly differentiated from the other members of the family Thermomonosporaceae and that they warranted separate genus status. We conclude that Actinomadura spadix should be assigned the status of the type species of a new genus as Actinoallomurus spadix gen. nov., comb. nov. (type strain NBRC 14099T=ATCC 27298T=BCRC 13386T=CBS 261.72T=CIP 105479T=DSM 43459T=JCM 3146T=KCTC 9252T=NCIMB 11118T=NRRL B-16128T). Further, we conclude that the ten new isolates should be assigned to the novel species Actinoallomurus amamiensis sp. nov. (type strain TT00-28T=NBRC 103682T=KCTC 19537T), Actinoallomurus caesius sp. nov. (type strain A3015T=NBRC 103678T=KCTC 19535T), Actinoallomurus coprocola sp. nov. (type strain TT04-09T=NBRC 103688T=KCTC 19542T), Actinoallomurus fulvus sp. nov. (type strain TT99-66T=NBRC 103680T=KCTC 19536T), Actinoallomurus iriomotensis sp. nov. (type strain TT02-47T=NBRC 103685T=KCTC 19539T), Actinoallomurus luridus sp. nov. (type strain TT02-15T=NBRC 103683T=KCTC 19538T), Actinoallomurus purpureus sp. nov. (type strain TTN02-30T=NBRC 103687T=KCTC 19541T) and Actinoallomurus yoronensis sp. nov. (type strain TTN02-22T=NBRC 103686T=KCTC 19540T). PMID:19567582

Tamura, Tomohiko; Ishida, Yuumi; Nozawa, Yuriko; Otoguro, Misa; Suzuki, Ken-ichiro

2009-08-01

100

SP1TFRE-SP-100 transient fuel rod evaluation code  

E-print Network

OF CONTENTS Page iv Vl 1 Vl i 1 CHAPTER I. INTRODUCTION CHAPTER II. COMPARISON OF PWR, BWR, I MFBR AND . SP-ioo TYPE REACTORS CHAPTER III. THERMAL ANALYSIS OF REACTOR FUEL PINS 20 i. Transient Heat Conduction Analysis 2. Pore Migration and Fuel... DEPRESSION . . APPENDIX G. CODE DESCRIPTION 115 118 121 123 126 LIST OF TABLES Table Page Major operating parameters of a BWR, PWR, LMFBR and SP-100 space reactor. 7 III-1 Summary of the Finite difference heat conduction equations 24 III-2 Var...

Carpenter, David Charles

2012-06-07

101

Nuclear Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP  

E-print Network

Nuclear Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP 16 hours Prereq- Math 130 or math ACT 28 Math 130 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 or Math SAT 630 EF 105

Grissino-Mayer, Henri D.

102

Aerospace Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU  

E-print Network

Aerospace Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT 28 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 or Math SAT 630 EF 105

Grissino-Mayer, Henri D.

103

Structural and functional characterization of the human and mouse fibulin-1 gene promoters: role of Sp1 and Sp3.  

PubMed Central

Fibulin-1 is a multifunctional extracellular protein involved in diverse biological processes including cardiovascular development, haemostasis and cancer. To investigate the transcriptional regulation of the gene encoding fibulin-1 we cloned and analysed about 4.0 kb of the 5'-flanking regions of both the human and mouse fibulin-1 genes. The human and mouse fibulin-1 promoters share little sequence similarity except for a short region of approx. 150-170 bp immediately upstream of the translation start site. The conserved region contains a TATA-like sequence (ATAATT) and multiple consensus binding sites for Sp1 and activator protein 2 (AP-2). That the short conserved region in each gene confers basal promoter activity is demonstrated by transient transfections of promoter deletion constructs for both the human and mouse genes into cells that express fibulin-1 constitutively. Co-transfections of promoter constructs with expression plasmids for Sp1, Sp3 and Sp4 into Drosophila SL2 cells indicate that Sp1 and Sp3 are essential for transcriptional activation and that these two factors act synergistically. Electrophoretic mobility-shift assays show that Sp1 and Sp3, but not AP-2, bind to the basal promoter of the human fibulin-1 gene. The results demonstrate the functional importance of Sp1 and Sp3 in regulating the expression of the fibulin-1 gene. PMID:11829738

Castoldi, Mirco; Chu, Mon-Li

2002-01-01

104

Infant formula alters surfactant protein A (SP-A) and SP-B expression in pulmonary epithelial cells.  

PubMed

Surfactant proteins A (SP-A) and SP-B are critical in the ability of pulmonary surfactant to reduce alveolar surface tension and provide innate immunity. Aspiration of infant milk formula can lead to lung dysfunction, but direct effects of aspirated formula on surfactant protein expression in pulmonary cells have not been described. The hypothesis that infant formula alters surfactant protein homeostasis was tested in vitro by assessing surfactant protein gene expression in cultured pulmonary epithelial cell lines expressing SP-A and SP-B that were transiently exposed (6 hr) to infant formula. Steady-state levels of SP-A protein and mRNA and SP-B mRNA in human bronchiolar (NCI-H441) and mouse alveolar (MLE15) epithelial cells were reduced in a dose-dependent manner 18 hr after exposure to infant formula. SP-A mRNA levels remained reduced 42 hr after exposure, but SP-B mRNA levels increased 10-fold. Neither soy formula nor non-fat dry milk affected steady-state SP-A and SP-B mRNA levels; suggesting a role of a component of infant formula derived from cow milk. These results indicate that infant formula has a direct, dose-dependent effect to reduce surfactant protein gene expression. Ultimately, milk aspiration may potentially result in a reduced capacity of the lung to defend against environmental insults. PMID:21520433

Chen, Maurice G; Atkins, Constance L; Bruce, Shirley R; Khan, Amir M; Liu, Yuying; Alcorn, Joseph L

2011-09-01

105

?-Caprolactam Utilization by Proteus sp. and Bordetella sp. Isolated From Solid Waste Dumpsites in Lagos State, Nigeria, First Report.  

PubMed

The ?-caprolactam is the monomer of the synthetic non-degradable nylon-6 and often found as nonreactive component of nylon-6 manufacturing waste effluent. Environmental consequences of its toxicity to natural habitats and humans pose a global public concern. Soil samples were collected from three designated solid waste dumpsites, namely, Abule-Egba, Olusosun and Isheri-Igando in Lagos State, Nigeria. Sixteen bacteria isolated from these samples were found to utilize the ?-caprolactam as a sole source of carbon and nitrogen at concentration of ?20 g l(-1). The isolates were characterized using their 16S rRNA gene sequence and showed similarity with Pseudomonas sp., Proteus sp., Providencia sp., Corynebacterium sp., Lysinibacillus sp., Leucobacter sp., Alcaligenes sp. and Bordetella sp. Their optimal growth conditions were found to be at temperature range of 30 to 35 °C and pH range of 7.0-7.5. High Performance liquid chromatography analysis of the ?-caprolactam from supernatant of growth medium revealed that these isolates have potential to remove 31.6-95.7 % of ?-caprolactam. To the best of our knowledge, this study is first to report the ability of Proteus sp. and Bordetella sp. for ?-caprolactam utilization. PMID:24426112

Sanuth, Hassan Adeyemi; Yadav, Amit; Fagade, Obasola Ezekiel; Shouche, Yogesh

2013-06-01

106

Effects of lung surfactant proteins, SP-B and SP-C, and palmitic acid on monolayer stability.  

PubMed Central

Langmuir isotherms and fluorescence and atomic force microscopy images of synthetic model lung surfactants were used to determine the influence of palmitic acid and synthetic peptides based on the surfactant-specific proteins SP-B and SP-C on the morphology and function of surfactant monolayers. Lung surfactant-specific protein SP-C and peptides based on SP-C eliminate the loss to the subphase of unsaturated lipids necessary for good adsorption and respreading by inducing a transition between monolayers and multilayers within the fluid phase domains of the monolayer. The morphology and thickness of the multilayer phase depends on the lipid composition of the monolayer and the concentration of SP-C or SP-C peptide. Lung surfactant protein SP-B and peptides based on SP-B induce a reversible folding transition at monolayer collapse that allows all components of surfactant to be retained at the interface during respreading. Supplementing Survanta, a clinically used replacement lung surfactant, with a peptide based on the first 25 amino acids of SP-B also induces a similar folding transition at monolayer collapse. Palmitic acid makes the monolayer rigid at low surface tension and fluid at high surface tension and modifies SP-C function. Identifying the function of lung surfactant proteins and lipids is essential to the rational design of replacement surfactants for treatment of respiratory distress syndrome. PMID:11325728

Ding, J; Takamoto, D Y; von Nahmen, A; Lipp, M M; Lee, K Y; Waring, A J; Zasadzinski, J A

2001-01-01

107

Early SP-100 flight mission designs  

NASA Astrophysics Data System (ADS)

Early flight mission objectives can be met with a Space Reactor Power System (SRPS) using thermoelectric conversion in conjunction with fast spectrum, lithium-cooled reactors. This paper describes two system design options using thermoelectric technology to accommodate an early launch. In the first of these options, radiatively coupled Radioiosotope Thermoelectric Generator (RTG) unicouples are adapted for use with a SP-100-type reactor heat source. Unicouples have been widely used as the conversion technology in RTGs and have demonstrated the long-life characteristics necessary for a highly relible SRPS. The thermoelectric leg height is optimized in conjunction with the heat rejection temperature to provide a mass optimum 6-kWe system configured for launch on a Delta II launch vehicle. The flight-demonstrated status of this conversion technology provides a high confidence that such a system can be designed, assembled, tested, and launched by 1997. The use of a SP-100-type reactor assures compliance with safety requirements and expedites the flight safety approval process while, at the same time, providing flight performance verification for a heat source technology with the growth potential to meet future national needs for higher power levels. A 15-kW2, Atlas IIAS-launched system using the compact, conductively coupled multicouple converters being developed under the SP-100 program to support an early flight system launch also described. Both design concepts have been scaled to 20-kWe in order to support recent studies by DOE/NASA for higher power early launch missions.

Josloff, Allan T.; Shepard, Neal F.; Kirpich, Aaron S.; Murata, Ronald; Smith, Michael A.; Stephen, James D.

1993-01-01

108

SP-100, a project manager's view  

NASA Technical Reports Server (NTRS)

Born to meet the special needs of America's space effort, the SP-100 Program testifies to the cooperation among government agencies. The Department of Energy (DOE), the National Aeronautics and Space Administration (NASA), and the Defense Advanced Research Projects Agency (DARPA) are working together to produce a 100-kW power system for use in outer space. At this point in the effort, it is appropriate to review: The approach to meet program goals; the status of activities of the Project Office, managed by the Jet Propulsion Laboratory (JPL); and, because this is a meeting on materials, answers beings developed by the Project Office to vital questions on refractory alloy technology.

Truscello, Vincent C.

1983-01-01

109

Amino Alcohols from the Ascidian Pseudodistoma sp  

PubMed Central

Seven new amino alcohol compounds, pseudoaminols A–G (1–7), were isolated from the ascidian Pseudodistoma sp. collected off the coast of Chuja-do, Korea. Structures of these new compounds were determined by analysis of the spectroscopic data and from chemical conversion. The presence of an N-carboxymethyl group in two of the new compounds (6 and 7) is unprecedented among amino alcohols. Several of these compounds exhibited moderate antimicrobial activity and cytotoxicity, as well as weak inhibitory activity toward Na+/K+-ATPase. PMID:24962272

Won, Tae Hyung; You, Minjung; Lee, So-Hyoung; Rho, Boon Jo; Oh, Dong-Chan; Oh, Ki-Bong; Shin, Jongheon

2014-01-01

110

SP-100 control drive assembly development  

NASA Technical Reports Server (NTRS)

The SP-100 is an electrical generating nuclear power system for space operation. This paper describes the nuclear reactor control systems and the methods used to assure reliable performance for the 10-year design life. Reliable performance is achieved by redundancy and by selecting highly reliable components and design features. Reliability is quantified by analysis using established reliability data. Areas lacking reliability data are identified for development testing. A specific development test description is provided as an example to demonstrate how this process is meeting the system reliability goals.

Gleason, Thomas; Gilchrist, A. Richard; Schuster, Gary

1993-01-01

111

SP-100 radiator design trade study  

NASA Technical Reports Server (NTRS)

A design trade study of the SP-100 heat rejection subsystem (HRSS) was made. A system code was used to evaluate the sensitivity of the HRSS mass and performance to changes. Variations in heat pipe diameter and cross-section, fin length and thickness, armor thickness, and overall configuration and materials were evaluated. The analysis indicates that the minimum system mass occurs for the case with many small diameter heat pipes, with ducting that maximizes the fraction of the heat pipe evaporator perimeter in contact with it.

Ewell, Richard

1992-01-01

112

Longidorus grandis n. sp. and L. paralongicaudatus n. sp. (Nematoda: Longidoridae), Two Parthenogenetic Species from Arkansas  

PubMed Central

Two new parthenogenetic species of Longidorus were found in Arkansas. Longidorus grandis n. sp. is characterized by its body (5.80-8.24 mm), slightly offset head, head width 20-27 µm, odontostyle 86-100 µm, guide ring 26-35 µm posterior to the anterior end, short conoid to mammiliform tail. Longidorus grandis n. sp. is similar to L. vineacola Sturhan &Weischer, 1964; L. lusitanicus Macara, 1985; L. edmundsi Hunt &Siddiqi, 1977; L. kuiperi Brinkman, Loof &Barbez, 1987; L. balticus Brzeski, Peneva &Brown, 2000; L. closelongatus Stoyanov, 1964; and L. seinhorsti Peneva, Loof &Brown, 1998. Longidorus paralongicaudatus n. sp. is characterized by its body length (2.60-5.00 µm), anteriorly flattened and offset head region 13-18 µm wide, odontostyle length 92-127 µm, guide ring 21-30 µm posterior to the anterior end, tail elongate-conical, and c' = 1.2-2.6. Longidorus paralongicaudatus n. sp. most closely resembles L. longicaudatus Siddiqi, 1962; L. socialis Singh &Khan, 1996; L. juvenilis Dalmasso, 1969; and L. curvatus Khan, 1986. PMID:19262768

Ye, Weimin; Robbins, R. T.

2003-01-01

113

Calcium Carbonate Formation by Synechococcus sp. Strain PCC 8806 and Synechococcus sp. Strain PCC 8807  

SciTech Connect

Precipitation of CaCO3 catalyzed by the growth and physiology of cyanobacteria in the Genus Synechococcus represents a potential mechanism for sequestration of CO2 produced during the burning of coal for power generation. Microcosm experiments were performed in which Synechococcus sp. strain PCC 8806 and Synechococcus sp. strain PCC 8807 were tested for their ability to calcify when exposed to a fixed calcium concentration of 3.4 mM and bicarbonate concentrations of 0.5, 1.25 and 2.5 mM. Disappearance of soluble calcium was used as an indicator of CaCO3 formation; results from metabolically active microcosms were compared to controls with no cells or no carbonate added. Synechococcus sp. strain PCC 8806 removed calcium continuously over the duration of the experiment with approximately 18.6 mg of calcium in the solid phase. Calcium removal occurred over a two-day time period when Synechococcus sp. strain PCC 8807 was tested and only 8.9 mg of calcium was removed in the solid phase. The ability of the cyanobacteria to create an alkaline growth environment appeared to be the primary factor responsible for CaCO3 precipitation in these experiments. Removal of inorganic carbon by fixation into biomass was insignificant compared to the mass of inorganic carbon removed by incorporation into the growing CaCO3 solid.

Lee, Brady D.; William A. Apel; Michelle R. Walton

2006-12-01

114

Clinical problems of sloths (Bradypus sp. and Choloepus sp.) in captivity.  

PubMed

A 20-yr retrospective study of disease prevalence was carried out for 51 sloths (34 Bradypus sp. and 17 Choloepus sp.) at the São Paulo Zoo. A total of 81 clinical disorders were detected, including nutritional (45.7%), digestive (12.3%), and respiratory (12.3%) problems and injuries (6.1%). A definitive diagnosis was not possible in 8.6% of the cases. The incidence of disease varied according to seasonal climate (winter, 32.5%; spring, 24%; summer, 22.9%; autumn, 20.5%), time in captivity (96.4% of diseases occurred within the first 6 mo and 3.6% occurred thereafter), and type of enclosure (quarantine cage, 96.4%; exhibition enclosure, 3.6%). Both young animals (86.7%) and adults (3.2%) were affected. Parasites were identified by fecal examination in 45.4% of animals with clinical illness (Ascaris sp., 80%; Coccidia sp., 20%). Bacteria such as Salmonella enteritidis, Escherichia coli, and Citrobacter freundii were isolated from feces and/or organs. The first 6 mo in captivity are critical for these animals. Proper management and early identification of medical conditions in captivity have implications for sloth population in the wild. PMID:10367647

Diniz, L S; Oliveira, P M

1999-03-01

115

Calcium carbonate formation by Synechococcus sp. strain PCC 8806 and Synechococcus sp. strain PCC 8807.  

PubMed

Precipitation of CaCO3 catalyzed by the growth and physiology of cyanobacteria in the genus Synechococcus represents a potential mechanism for sequestration of atmospheric CO2 produced during the burning of coal for power generation. Synechococcus sp. strain PCC 8806 and Synechococcus sp. strain PCC 8807 were tested in microcosm experiments for their ability to calcify when exposed to a fixed calcium concentration of 3.4 mM and dissolved inorganic carbon concentrations of 0.5, 1.25 and 2.5 mM. Synechococcus sp. strain PCC 8806 removed calcium continuously over the duration of the experiment producing approximately 18.6 mg of solid phase calcium. Calcium removal occurred over a two-day time period when Synechococcus sp. strain PCC 8807 was tested and only 8.9 mg of solid phase calcium was produced. Creation of an alkaline growth environment catalyzed by the physiology of the cyanobacteria appeared to be the primary factor responsible for CaCO3 precipitation in these experiments. PMID:16289626

Lee, Brady D; Apel, William A; Walton, Michelle R

2006-12-01

116

IBM SP high-performance networking with a GRF.  

SciTech Connect

Increasing use of highly distributed applications, demand for faster data exchange, and highly parallel applications can push the limits of conventional external networking for IBM SP sites. In technical computing applications we have observed a growing use of a pipeline of hosts and networks collaborating to collect, process, and visualize large amounts of realtime data. The GRF, a high-performance IP switch from Ascend and IBM, is the first backbone network switch to offer a media card that can directly connect to an SP Switch. This enables switch attached hosts in an SP complex to communicate at near SP Switch speeds with other GRF attached hosts and networks.

Navarro, J.P.

1999-05-27

117

Auxiliary-assisted palladium-catalyzed arylation and alkylation of sp2 and sp3 carbon-hydrogen bonds.  

PubMed

We have developed a method for auxiliary-directed, palladium-catalyzed beta-arylation and alkylation of sp(3) and sp(2) C-H bonds in carboxylic acid derivatives. The method employs a carboxylic acid 2-methylthioaniline- or 8-aminoquinoline amide substrate, aryl or alkyl iodide coupling partner, palladium acetate catalyst, and an inorganic base. By employing 2-methylthioaniline auxiliary, selective monoarylation of primary sp(3) C-H bonds can be achieved. If arylation of secondary sp(3) C-H bonds is desired, 8-aminoquinoline auxiliary may be used. For alkylation of sp(3) and sp(2) C-H bonds, 8-aminoquinoline auxiliary affords the best results. Some functional group tolerance is observed and amino- and hydroxy-acid derivatives can be functionalized. Preliminary mechanistic studies have been performed. A palladacycle intermediate has been isolated, characterized by X-ray crystallography, and its reactions have been studied. PMID:20175511

Shabashov, Dmitry; Daugulis, Olafs

2010-03-24

118

The Effect of Acupuncture to SP6 on Skin Temperature Changes of SP6 and SP10: An Observation of “Deqi”  

PubMed Central

Background. Deqi sensation is a complex but an important component for acupuncture effect. In this study, we tried to observe the relationship between Deqi and skin temperature changes and whether there was some relativity between Deqi and needle stimulations on cold congealing and dysmenorrhea rat model. Thirty-two female Sprague Dawley (SD) rats were randomly divided into four groups (Saline Control Group, Model Group, Group A with strong stimulation, and Group B with small stimulation). Group A and Group B were performed with different stimulations. We found that, compared with saline control group, model group, and Group B, Group A showed that the skin temperature changes on right acupoint SP6 and SP10 increased significantly at 5?min–10?min interval. The skin temperature changes on left SP6 decreased at instant–5?min interval. The skin temperature changes on right SP10 decreased significantly at instant–5?min interval and 10?min–20?min interval. Thermogenic action along Spleen Meridian of Foot Greater Yin was manifested as simultaneous skin temperature increase on right SP6 and SP10 at 5?min–10?min interval after needling SP6, which was helpful to illustrate the relationship between the characteristic of Deqi and needle stimulations. PMID:24665334

Yang, Jia-Min; Shen, Xiao-Yu; Shen, Song-Xi; Qi, Dan-Dan; Luo, Li; Ren, Xiao-Xuan; Ji, Bo; Zhang, Lu-Fen; Li, Xiao-Hong; Zhu, Jiang

2014-01-01

119

The Effect of Acupuncture to SP6 on Skin Temperature Changes of SP6 and SP10: An Observation of "Deqi".  

PubMed

Background. Deqi sensation is a complex but an important component for acupuncture effect. In this study, we tried to observe the relationship between Deqi and skin temperature changes and whether there was some relativity between Deqi and needle stimulations on cold congealing and dysmenorrhea rat model. Thirty-two female Sprague Dawley (SD) rats were randomly divided into four groups (Saline Control Group, Model Group, Group A with strong stimulation, and Group B with small stimulation). Group A and Group B were performed with different stimulations. We found that, compared with saline control group, model group, and Group B, Group A showed that the skin temperature changes on right acupoint SP6 and SP10 increased significantly at 5?min-10?min interval. The skin temperature changes on left SP6 decreased at instant-5?min interval. The skin temperature changes on right SP10 decreased significantly at instant-5?min interval and 10?min-20?min interval. Thermogenic action along Spleen Meridian of Foot Greater Yin was manifested as simultaneous skin temperature increase on right SP6 and SP10 at 5?min-10?min interval after needling SP6, which was helpful to illustrate the relationship between the characteristic of Deqi and needle stimulations. PMID:24665334

Yang, Jia-Min; Shen, Xiao-Yu; Zhang, Ling; Shen, Song-Xi; Qi, Dan-Dan; Zhu, Shi-Peng; Luo, Li; Ren, Xiao-Xuan; Ji, Bo; Zhang, Lu-Fen; Li, Xiao-Hong; Zhu, Jiang

2014-01-01

120

SP-100 liquid metal test loop design  

NASA Astrophysics Data System (ADS)

The SP-100 Power System Qualification (PSO) program validates the technology readiness of the SP-100 Generic Flight System (GFS). As part of the PSQ, the GFS reactor, heat transport and power generation systems are being validated, by test, in high temperature liquid metal test loops. The liquid metal test loop program consists of two test loops. The first, a natural circulation material test loop (MTL), has been successfully operating for the last year at GE's test facility in San Jose. The second, a forced circulation Component Test Loop (CTL) is in the preliminary design phase. Fabrication of the CTL and modifications to the Test Facility will be completed in FY94 with component testing scheduled to begin in FY95. The CTL is a Nb-1Zr test loop with an Electromagnetic (EM) pump providing forced circulation for the liquid lithium coolant. The CTL test program is comprised of a series of individual component tests. Test components containing thermoelectric cells will have their cold side ducts piped to an existing heat rejection loop external to the CTL vacuum vessel. The test assembly and test components are being designed by GE. The detail design of several loop components is being performed by Westinghouse Atomic Energy Systems (WAES). The CTL will be assembled and the test performed at GE's facilties in San Jose, California.

Fallas, T. Ted; Kruger, Gordon B.; Wiltshire, Frank R.; Jensen, Grant C.; Clay, Harold; Upton, Hugh A.; Gamble, Robert E.; Kjaer-Olsen, Christian; Lee, Keith

1992-01-01

121

The ANL/IBM SP scheduling system  

SciTech Connect

Approximately five years ago scientists discovered that modern UNLX workstations connected with ethernet and fiber networks could provide enough computational performance to compete with the supercomputers. As this concept became increasingly popular, the need for distributed queuing and scheduler systems became apparent. Systems such as DQS from Florida State University were developed and worked very well. Today however, supercomputers such as Argonne National Laboratory`s IBM SP system can provide more CPU and networking speed than can be obtained from these networks of workstations. Nevertheless, because modern super computers look like clusters of workstations developers felt that the scheduling systems previously used on clusters of workstations should still apply. After trying to apply some of these scheduling systems to Argonne`s SP environment it became obvious that these two computer environments have very different scheduling needs. Recognizing this need, and realizing that no one has addressed it, we at Argonne developed a new scheduling system. The approach taken in creating this system was unique in that user input and interaction were encouraged throughout the development process. Thus a scheduler was built that actually works the way the users want it to.

Lifka, D.

1995-02-01

122

Arsenite Oxidase from Ralstonia sp. 22  

PubMed Central

We characterized the aro arsenite oxidation system in the novel strain Ralstonia sp. 22, a ?-proteobacterium isolated from soil samples of the Salsigne mine in southern France. The inducible aro system consists of a heterodimeric membrane-associated enzyme reacting with a dedicated soluble cytochrome c554. Our biochemical results suggest that the weak association of the enzyme to the membrane probably arises from a still unknown interaction partner. Analysis of the phylogeny of the aro gene cluster revealed that it results from a lateral gene transfer from a species closely related to Achromobacter sp. SY8. This constitutes the first clear cut case of such a transfer in the Aro phylogeny. The biochemical study of the enzyme demonstrates that it can accommodate in vitro various cytochromes, two of which, c552 and c554, are from the parent species. Cytochrome c552 belongs to the sox and not the aro system. Kinetic studies furthermore established that sulfite and sulfide, substrates of the sox system, are both inhibitors of Aro activity. These results reinforce the idea that sulfur and arsenic metabolism are linked. PMID:20421652

Lieutaud, Aurélie; van Lis, Robert; Duval, Simon; Capowiez, Line; Muller, Daniel; Lebrun, Régine; Lignon, Sabrina; Fardeau, Marie-Laure; Lett, Marie-Claire; Nitschke, Wolfgang; Schoepp-Cothenet, Barbara

2010-01-01

123

Profileringsvakken in Bachelor Wiskunde SP 33 ESP-MICE-14054 Operationeel onderzoek -Operational Research  

E-print Network

Profileringsvakken in Bachelor Wiskunde SP 33 ESP-MICE-14054 Operationeel onderzoek - Operational Research SP6 ESP-BEDR-2470 Economie en bedrijfsleven - Business and Economics SP3 ESP-ACCO-2405 Fiscaliteit - Tax Law SP6 ESP-ACCO-11214 Ondernemingsrecht - Company Law SP6 ESP-MICE-14054 Algemeen boekhouden en

Einmahl, Uwe

124

Lung Surfactant Protein A (SP-A) Interactions with Model Lung Surfactant Lipids and an SP-B Fragment  

PubMed Central

Surfactant protein A (SP-A) is the most abundant protein component of lung surfactant, a complex mixture of proteins and lipids. SP-A performs host defense activities and modulates the biophysical properties of surfactant in concerted action with surfactant protein B (SP-B). Current models of lung surfactant mechanism generally assume SP-A functions in its octadecameric form. However, one of the findings of this study is that when SP-A is bound to detergent and lipid micelles that mimic lung surfactant phospholipids, it exists predominantly as smaller oligomers, in sharp contrast to the much larger forms observed when alone in water. These investigations were carried out in sodium dodecyl sulfate (SDS), dodecylphosphocholine (DPC), lysomyristoylphosphatidylcholine (LMPC), lysomyristoylphosphatidylglycerol (LMPG), and mixed LMPC + LMPG micelles, using solution and diffusion nuclear magnetic resonance (NMR) spectroscopy. We have also probed SP-A’s interaction with Mini-B, a biologically active synthetic fragment of SP-B, in the presence of micelles. Despite variations in Mini-B’s own interactions with micelles of different compositions, SP-A is found to interact with Mini-B in all micelle systems and perhaps to undergo a further structural rearrangement upon interacting with Mini-B. The degree of SP-A–Mini-B interaction appears to be dependent on the type of lipid headgroup and is likely mediated through the micelles, rather than direct binding. PMID:21553841

2011-01-01

125

Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains  

PubMed Central

Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch. PMID:19547659

Sioud, Samiha; Aigle, Bertrand; Karray-Rebai, Ines; Smaoui, Slim; Bejar, Samir; Mellouli, Lotfi

2009-01-01

126

Two SP-C genes encoding human pulmonary surfactant proteolipid.  

PubMed

Human pulmonary surfactant proteolipid of Mr = 5,000, now termed surfactant protein C (SP-C), is produced by proteolytic processing of an Mr = 22,000 precursor. The active hydrophobic peptide imparts surface active properties to pulmonary surfactant phospholipids. We have determined the entire nucleotide sequence of two distinct genes encoding SP-C from a genomic library prepared from human leukocytes. SP-C genes were encoded by approximately 3.0 kilobase pairs of DNA containing six exons and five introns. In both genes, the active hydrophobic region of the polypeptide was located in the second exon that encodes a peptide of 53 amino acids. The entire nucleotide sequences of the two classes of SP-C genes differed by only 1%. Two cDNAs encoding SP-C were distinguished on the basis of an 18-nucleotide deletion at the beginning of the fifth exon; no such deletion was detected within the two classes of SP-C genes. Comparison of the 3'-untranslated regions of SP-C cDNA clones and the two classes of genomic clones demonstrated that cDNAs with and without the 18-base pair deletion could be derived from both of the genes. This 18-base pair deletion occurs in nucleotide sequences compatible with two distinct RNA splice sites. One additional cDNA clone showed the addition of an 8-base pair insert at the end of exon 5, which was also compatible with two distinct splice sites. Both classes of SP-C genes were represented by cDNAs, demonstrating that both classes of genes are actively transcribed. The two SP-C genes were readily distinguished on the basis of their nucleotide sequences and restriction fragment analyses of their flanking DNA. Two distinct classes of human SP-C genes are transcribed, and the heterogeneity in the SP-C RNAs appears to result from differential splicing. PMID:2839484

Glasser, S W; Korfhagen, T R; Perme, C M; Pilot-Matias, T J; Kister, S E; Whitsett, J A

1988-07-25

127

Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus Rahnella.  

PubMed

Isolations from oak symptomatic of Acute Oak Decline, alder and walnut log tissue, and buprestid beetles in 2009-2012 yielded 32 Gram-negative bacterial strains showing highest gyrB sequence similarity to Rahnella aquatilis and Ewingella americana. Multilocus sequence analysis (using partial gyrB, rpoB, infB and atpD gene sequences) delineated the strains into six MLSA groups. Two MLSA groups contained reference strains of Rahnella genomospecies 2 and 3, three groups clustered within the Rahnella clade with no known type or reference strains and the last group contained the type strain of E. americana. DNA-DNA relatedness assays using both the microplate and fluorometric methods, confirmed that each of the five Rahnella MLSA groups formed separate taxa. Rahnella genomospecies 2 and 3 were previously not formally described due to a lack of distinguishing phenotypic characteristics. In the present study, all five Rahnella MLSA groups were phenotypically differentiated from each other and from R. aquatilis. Therefore we propose to classify the strains from symptomatic oak, alder and walnut and buprestid beetles as: Rahnella victoriana sp. nov. (type strain FRB 225(T)=LMG 27717(T)=DSM 27397(T)), Rahnella variigena sp. nov. (previously Rahnella genomosp. 2, type strain CIP 105588(T)=LMG 27711(T)), Rahnella inusitata sp. nov. (previously Rahnella genomosp. 3, type strain DSM 30078(T)=LMG 2640(T)), Rahnella bruchi sp. nov. (type strain FRB 226(T)=LMG 27718(T)=DSM 27398(T)) and Rahnella woolbedingensis sp. nov. (type strain FRB 227(T)=LMG 27719(T)=DSM 27399(T)). PMID:25264035

Brady, Carrie; Hunter, Gavin; Kirk, Susan; Arnold, Dawn; Denman, Sandra

2014-12-01

128

Progress in the SP-100 FSQ reactor development  

NASA Astrophysics Data System (ADS)

The reactor for the SP-100 Space Power Supply System is described in this paper. The various components that make up the reactor are discussed and the testing programs to assure that the design will meet the SP-100 requirements are described.

Parker, M. B.

1991-09-01

129

LOCALIZATION OF SP22 ON HUMAN SPERM OF DIFFERING QUALITY  

EPA Science Inventory

LOCALIZATION OF SP22 ON HUMAN SPERM OF DIFFERING QUALITY. AE Lavers*1, GR Klinefelter2, DW Hamilton1, KP Roberts1, 1University of Minnesota, Minneapolis, MN and 2US EPA, Research Triangle Park, NC. SP22 is a sperm membrane protein that has been implicated in sperm function d...

130

Thermogravimetric characteristics and pyrolysis kinetics of alga Sagarssum sp. biomass.  

PubMed

Alga Sagarssum sp. can be converted to bio-oil, gas, and char through pyrolysis. In this study, the pyrolysis characteristics and kinetics of Sagarssum sp. were investigated using a thermogravimetric analyzer and tubing reactor, respectively. Sagarssum sp. decomposed below 550°C, but the majority of materials decomposed between 200 and 350°C at heating rates of 5-20°C/min. The apparent activation energy increased from 183.53 to 505.57 kJ mol(-1) with increasing pyrolysis conversion. The kinetic parameters of Sagarssum sp. pyrolysis were determined using nonlinear least-squares regression of the experimental data, assuming second-order kinetics. The proposed lumped kinetic model represented the experimental results well and the kinetic rate constants suggested a predominant pyrolysis reaction pathway from Sagarssum sp. to bio-oil, rather than from Sagarssum sp. to gas. The kinetic rate constants indicated that the predominant reaction pathway was A (Sagarssum sp.) to B (bio-oil), rather than A (Sagarssum sp.) to C (gas; C1-C4). PMID:23665684

Kim, Seung-Soo; Ly, Hoang Vu; Kim, Jinsoo; Choi, Jae Hyung; Woo, Hee Chul

2013-07-01

131

Nitrogen Control of Atrazine Utilization in Pseudomonas sp. Strain ADP  

Microsoft Academic Search

Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that support rapid growth of Pseudomonas sp. strain ADP compared to cells cultivated

V. Garcia-Gonzalez; Fernando Govantes; Liz J. Shaw; Richard G. Burns; Eduardo Santero

2003-01-01

132

Sarcocystis SP. IN THE EASTERN COTTONTAIL (Sylvilagus floridanus)  

Microsoft Academic Search

Sarcocystis sp. was observed in 100 of 185 (54.1%) Eastern cottontails (Sylvilagus floridanus) examined in Pennsylvania over a three year period. Gross and histologic examination commonly revealed cysts in skeletal muscle of the fore and hind legs, flanks and loins. Two rabbits had cysts in esophageal skeletal muscle. Host response to Sarcocystis sp. is described. Adult rabbits had a significantly

M. COSGROVE; J. P. WIGGINS; H. ROTHENBACHER

1982-01-01

133

SP100 ground engineering system nuclear assembly test  

Microsoft Academic Search

SP-100 is a space power system that is being developed by the General Electric Company to meet future space electrical power requirements. The ground testing of an SP-100 prototypical reactor system will be conducted at the Westinghouse Hanford Company site located at Richland, Washington. Westinghouse Hanford will design and supply all associated site facility support systems. General Electric will supply

G. B. Kruger; M. K. Mahaffey

1989-01-01

134

SP100 lunar surface power system conceptual design  

Microsoft Academic Search

This paper describes a conceptual design study addressing the application of SP-100 Space Reactor Power System technology to a Lunar Surface Power System (LSPS). Key functional, design and interface requirements are defined for the lunar outpost application. A conceptual design is presented for a 100 kWe LSPS that builds on the technology and design for the orbital SP-100 Generic Flight

Hubert A. Upton; A. Richard Gilchrist; Robert Protsik; Robert E. Gamble; David W. Lunsford; Ian R. Temmerson

1992-01-01

135

SP100 first demonstration flight mission concept design  

Microsoft Academic Search

A conceptual design study was performed to define a SP-100 Space Reactor Power System (SRPS) compatible with a set of proposed first demonstration flight objectives and constraints. The principal mission objective is the confirmation and demonstration of the SP-100 technology and its design application maturity for space missions. The objective is to be achieved by SRPS assembly, ground acceptance test,

Dennis Switick; Charles Cowan; Darryl Hoover; Thomas Marcille; Robert Otwell; Neal Shepard

1992-01-01

136

SP100 multimegawatt scaleup to meet electric propulsion mission requirements  

Microsoft Academic Search

The SP-100 nuclear heat source technology, utilizing uranium nitride fuel clad in PWC-11 in a fast reactor with lithium coolant circulated by an electromagnetic pump, is shown to be directly extrapolatable to thermal power levels that meet NASA nuclear electric propulsion requirements using different power conversion techniques. The SP-100 nuclear technology can be applied to missions with NEP (nuclear electric

D. W. Newkirk; S. A. Salamah; S. L. Stewart; P. R. Pluta

1991-01-01

137

Start-up control strategy in the SP100  

Microsoft Academic Search

A control analysis was performed to evaluate the reference and two alternative reactor start-up control strategies for the SP-100, using a detailed nonlinear model of the reactor. The analysis results show that the reference control strategy for the SP-100 adequately meets the current requirements. Two alternative control strategies which are described in this paper provide tighter control than the reference

John E. Savanyo; Sang K. Rhow; Raymond A. Meyer; Brian F. Coleman; Frank J. Halfen

1991-01-01

138

SP100 technology scales from kilowatts to megawatts  

Microsoft Academic Search

This paper documents the nuclear system mass variable, its components, and related overall dimensions to the thermal power level of the SP-100 system. Additional advances in the SP-100 technologies are identified that will allow the nuclear-related subsystems to be scaled over a broad range of thermal powers.

Nelson A. Deane; Robert Protsik; Thomas F. Marcille; Darryl G. Hoover

1992-01-01

139

INTERNATIONAL SYSTEM OF NOMENCLATURE FOR PUCCINIA GRAMINIS F. SP. AVENAE  

Technology Transfer Automated Retrieval System (TEKTRAN)

Current systems that describe the virulence phenotype in Puccinia graminis f. sp. avenae have no systematic approach in the naming of races or in fully characterizing their virulence. A new nomenclature system that simply and fully characterizes virulence in P. graminis f. sp. avenae is proposed. Th...

140

Genome Sequence of Pectobacterium sp. Strain SCC3193  

PubMed Central

We report the complete and annotated genome sequence of the plant-pathogenic enterobacterium Pectobacterium sp. strain SCC3193, a model strain isolated from potato in Finland. The Pectobacterium sp. SCC3193 genome consists of a 516,411-bp chromosome, with no plasmids. PMID:23045508

Koskinen, J. Patrik; Laine, Pia; Niemi, Outi; Nykyri, Johanna; Harjunpää, Heidi; Auvinen, Petri; Paulin, Lars; Pirhonen, Minna; Palva, Tapio

2012-01-01

141

Analysis of SP-100 critical experiments  

SciTech Connect

In support of the SP-100 space nuclear power source program, preliminary critical benchmark experiments were performed at the ZPPR facility at ANL-W. These configurations are representative of small, fast-spectrum, BeO-reflected, liquid metal-cooled space reactor designs at a 300-kWe power level. Analyses were performed using MCNP (Monte Carlo) and TWODANT (discrete ordinates) transport codes to calculate system criticality, control worth, and power distribution. Both methods calculated eigenvalues within 0.5% of the experimental results. Internal-poison-rod worth was underpredicted and radial reflector worth was overpredicted by both codes by up to 20%. MCNP-calculated control drum worths were underestimated by approximately 8%. Good agreement with experimental values was observed for /sup 235/U fission and for /sup 238/U fission and capture rates with the best agreement occurring in the fuel region and slightly poorer predictions apparent near BeO moderator. 7 refs., 12 figs.

Sapir, J.L.; Brandon, D.I.; Collins, P.J.; Cowan, C.L.; Porter, C.A.; Andre, S.V.

1988-01-01

142

[Denitrogenation of petroleum by a Pseudomonas sp].  

PubMed

Biodenitrogenation of petroleum oil was investigated by a previously isolated carbazole-degrader Pseudomonas sp. XLDN4-9. In a tetradecane-aqueous phase system, biodegradation of carbazole was enhanced by the presence of n-tetradecane. And strain XLDN4-9 was capable of absorbing 95.2% of 2 g/L carbazole dissolved in diesel within 15 hours. Significant denitrogentation of crude oil, diesel and lubricanting oil was detected by strain XLDN4-9. Removal of carbazole, methylcarbazole, and dimethylcarbazole in diesel was confirmed by using GC-MS. After 3 days, 99% of carbazole and 15% of dimethyl carbazole was degraded. And the removal rate of 1-, 2-, 3-, and 4-methyl carbazole was determined to be 63.4%, 87.6%, 78.4%, and 66.5% respectively. PMID:18807996

Li, Li; Xu, Ping; Shi, Quan

2008-06-01

143

Winamp 1.92-SP1  

NSDL National Science Digital Library

Winamp 1.92-SP1, created by Nullsoft, Inc., is a fast, easy-to-use, hi-fidelity music player for Windows 95/98/NT. The player supports numerous audio file formats, most notably MPEG Audio Layer 3 (MP3), which allows near CD-quality sound while compressing a four-minute song into approximately four megabytes. The player has an intuitive interface which includes an equalizer, multi-song programming, and other useful controls. MP3 is becoming widely used as a quality audio compression standard and Winamp is an excellent audio player for use with MP3 and other formats. Winamp is shareware for the Windows 95/98/NT platforms and may be used free of charge for fourteen days, after which time a $10 registration fee is required.

144

SP-100 fuel pin performance analysis  

NASA Astrophysics Data System (ADS)

A modified version of the LIFE code was used to evaluate the performance of a wide range of UO2 and UN fuel pin designs for SP-100 reactors. The analyses covered the available irradiation data on refractory alloy-clad fuel pins and fuel pins for thermoelectric, thermionic, and Stirling engine applications. The LIFE code studies showed that vented UO2 fuel pins with W-26 percent Re cladding can operate with cladding temperatures up to the 1800 K for thermionic reactor applications. At cladding temperatures up to 1500 K, W-26 percent Re and ASTAR-811C cladding can be used for vented or nonvented UO2 fuel pins. Nb-1 percent Zr cladding is not recommended for nonvented pin designs at 1500 K. Similarly, for UN fuel, W-26 percent Re and ASTAR-811C cladding can be used up to 1500 K, whereas, T-111 and Nb-1 percent Zr are limited to temperatures below 1400 K.

Boltax, A.; Sundquist, B. E.

145

Benhamycin, novel alkaloid from terrestrial Streptomyces sp.  

PubMed

During our screening for bioactive natural compounds from microorganisms, a novel alkaloid has been isolated from a terrestrial Streptomyces sp. isolate NR12, and named as benhamycin (1). This was along with the known metabolites, uracil, thymine, p-hydroxybenzoic acid, 2'-deoxyuridin, tryptophol, indolyl-3-carboxylic acid, and indolyl-3-carbaldehyde. Chemical structure of the novel compound was determined by detailed analysis of its spectroscopic data (extensive NMR experiments, 1 & 2D, MS spectroscopy, and MS high resolution). Structurally, Benhamycin (1) is a pentacyclic aromatic compound bearing an acridine moiety lactamized with benzene. Biological studies showed that the strain extract was moderately active against Gram-positive, Gram-negative bacteria and fungi. PMID:17987502

Shaaban, Mohamed; Abdel-Aziz, Mohamed S

2007-11-01

146

Biodegradation of Xanthan Gum by Bacillus sp  

PubMed Central

Strains tentatively identified as Bacillus sp. were isolated from sewage sludge and soil and shown to elaborate extracellular enzymes that degrade the extracellular polysaccharide (xanthan gum, polysaccharide B-1459) of Xanthomonas campestris NRRL B-1459. Enzyme production by one strain was greatly enhanced when the strain was incubated in a mixed culture. Products of degradation were identified as d-glucuronic acid, d-mannose, pyruvylated mannose, 6-O-acetyl d-mannose, and a (1?4)-linked glucan. These products correlate with the known structure of the gum. The complexity of the product mixture indicated that the xanthanase was a mixture of carbohydrases. The xanthanase complexes were similar to one another in temperature stability, pH and temperature optima, degree of substrate degradation, and enzymolysis products. Differences in pH stability, salt tolerance, recoverability, and yields of enzyme were observed. PMID:16346068

Cadmus, Martin C.; Jackson, Linda K.; Burton, Kermit A.; Plattner, Ronald D.; Slodki, Morey E.

1982-01-01

147

Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp.nov., novel actinomycetes from soil.  

PubMed

The generic position of two aerobic, Gram-positive, non-acid-alcohol-fast actinomycetes was established following the isolation of their PCR-amplified 16S rRNA genes and alignment of the resultant sequences with the corresponding sequences from representatives of the families Actinosynnemataceae and Pseudonocardiaceae. The assignment of the organisms to the genus Saccharopolyspora was strongly supported by chemotaxonomic and morphological data. The strains were distinguished both from one another and from representatives of validly described Saccharopolyspora species on the basis of a number of phenotypic properties. It is proposed that the organisms, strains 07T (= AS4.1520T = IFO 16345T = JCM 10665T) and 216T (= AS4.1511T = IFO 16346T = JCM 10664T), be classified in the genus Saccharopolyspora as Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp. nov., respectively. PMID:11321076

Lu, Z; Liu, Z; Wang, L; Zhang, Y; Qi, W; Goodfellow, M

2001-03-01

148

ABSENCE OF THE SP/SP RECEPTOR CIRCUITRY IN THE SP PRECURSOR KNOCKOUT MICE OR SP-RECEPTOR, NEUROKININ (NK1) KNOCKOUT MICE LEADS TO AN INHIBITED CYTOKINE RESPONSE IN GRANULOMAS ASSOCIATED WITH MURINE TAENIA CRASSICEPS INFECTION  

PubMed Central

Neurocysticercosis, caused by the cestode Taenia solium, is the most common parasitic infection of the human central nervous system that leads to seizures. Taenia crassiceps cysticercosis in mice is an experimental model for Taenia solium cysticercosis. Similar to the human infection, live parasites cause little or no granulomatous inflammation. Dying parasites initiate a granulomatous reaction. The neuropeptide, Substance P (SP), stimulates Th1 cytokine production. In the current studies, we determined if absence of SP/SP receptor circuitry in the SP precursor, preprotachykinin knockout or SP-receptor, neurokinin (NK1) knockout mice, affected granuloma cytokine production. We hence compared the levels of Th1 cytokines, IL-2 and IFN-?, and levels of Th2/immunoregulatory cytokines, IL-4 and IL-10, by ELISA in T. crassiceps-induced granulomas derived from infected C57BL/6 wild type (WT) versus SP-Precursor knockout and NK1 knockout mice. We found that mean levels of IL-2, IFN-?, IL-4, and IL-10 in infected, WT-derived granulomas were significantly higher than those of granulomas derived from infected SP-Precursor knockout or the NK1 receptor knockout mice. Levels of Th2/immunoregulatory cytokines, IL-4 and IL-10, were higher in early stage granulomas (histologically-staged on basis of evidence of parasite remnants) versus late stage granulomas (no parasite-remnants) of both knockouts, whereas the reverse was noted in WT-derived granulomas. These studies established that the absence of an SP/SP receptor circuitry in the SP precursor knockout mice or NK1 receptor knockout led to an inhibited cytokine response. PMID:18576810

Garza, Armandina; Weinstock, Joel; Robinson, Prema

2008-01-01

149

Two novel species of marine phototrophic Gammaproteobacteria: Thiorhodococcus bheemlicus sp. nov. and Thiorhodococcus kakinadensis sp. nov.  

PubMed

Two coccoid phototrophic purple sulfur bacteria were isolated from marine habitats (marine aquaculture pond near Bheemli, Visakhapatnam and marine tidal waters from a fishing harbour, Kakinada) in a medium that contained 3% NaCl (w/v). Strains JA132T and JA130T are Gram-negative, motile cocci with a single flagellum. Both have an obligate requirement for NaCl. Intracellular photosynthetic membranes are of the vesicular type. Bacteriochlorophyll a and most probably carotenoids of the spirilloxanthin series were present as photosynthetic pigments. Both strains were able to grow photolithoautotrophically and photolithoheterotrophically. Chemotrophic and fermentative growth could not be demonstrated. There is no vitamin requirement for strain JA132T, while strain JA130T requires niacin, biotin and pantothenate as growth factors. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that both strains cluster with species of the genus Thiorhodococcus belonging to the Gammaproteobacteria. The DNA G+C contents of strains JA132T and JA130T were 65.5 and 57.5 mol%, respectively. Based on the 16S rRNA gene sequence analysis, morphological and physiological characteristics, strains JA132T and JA130T are significantly different from each other and from other species of the genus Thiorhodococcus and are recognized as two novel species, for which the names Thiorhodococcus bheemlicus sp. nov. and Thiorhodococcus kakinadensis sp. nov. are proposed. The type strains of T. bheemlicus sp. nov. and T. kakinadensis sp. nov. are JA132T (=MTCC 8120T=ATCC BAA-1362T=JCM 14149T=DSM 18805T) and JA130T (=ATCC BAA-1353T=DSM 18858T=JCM 14150T), respectively. PMID:17978199

Anil Kumar, P; Sasi Jyothsna, T S; Srinivas, T N R; Sasikala, Ch; Ramana, Ch V; Imhoff, J F

2007-11-01

150

Repression of the Luteinizing Hormone Receptor Gene Promoter by Cross Talk among EAR3\\/COUP-TFI, Sp1\\/Sp3, and TFIIB  

Microsoft Academic Search

Transcription of luteinizing hormone receptor (LHR) gene is activated by Sp1\\/Sp3 at two Sp1 sites and is repressed by nuclear orphan receptors EAR2 and EAR3 through a direct-repeat (DR) motif. To elucidate the mechanism of the orphan receptor-mediated gene repression, we explored the functional connection between the orphan receptors and Sp1\\/Sp3 complex, and its impact on the basal transcription machinery.

Ying Zhang; Maria L. Dufau

2003-01-01

151

Fusarium euwallaceae sp. nov.—a symbiotic fungus of Euwallacea sp., an invasive ambrosia beetle in Israel and California  

Technology Transfer Automated Retrieval System (TEKTRAN)

The invasive Asian ambrosia beetle Euwallacea sp. (Coleoptera, Scolytinae, Xyleborini) and a novel Fusarium sp. that it farms in its galleries as a source of nutrition seriously damage over 20 species of live trees and pose a serious threat to avocado production (Persea americana) in Israel and Cali...

152

for hsp60 and hsp70 of the anaerobic fungi Neocallimastix sp. L2 and Piromyces sp.  

E-print Network

for hsp60 and hsp70 of the anaerobic fungi Neocallimastix sp. L2 and Piromyces sp. E2. Hsp60- cherichia coli, filamentous fungi and yeast. R Durand, C Rascle, M FèvreR Durand, C Rascle, M Fèvre, Bdt 405, Universite Claude Bernard Lyon I, 69622 Villeurbanne Cedex, France) Anaerobic fungi

Paris-Sud XI, Université de

153

Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used for perchlorate reduction by Azospira sp.  

E-print Network

Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used was shown to be intrinsically different from the enzyme responsible for chlorate and perchlorate [(per)chlo- rate] reduction produced by Azospira sp. KJ based on subunit composition and other enzyme properties

154

Scalability of Parallel Spatial Direct Numerical Simulations on Intel Hypercube and IBM SP1 and SP2  

NASA Technical Reports Server (NTRS)

The implementation and performance of a parallel spatial direct numerical simulation (PSDNS) approach on the Intel iPSC/860 hypercube and IBM SP1 and SP2 parallel computers is documented. Spatially evolving disturbances associated with the laminar-to-turbulent transition in boundary-layer flows are computed with the PSDNS code. The feasibility of using the PSDNS to perform transition studies on these computers is examined. The results indicate that PSDNS approach can effectively be parallelized on a distributed-memory parallel machine by remapping the distributed data structure during the course of the calculation. Scalability information is provided to estimate computational costs to match the actual costs relative to changes in the number of grid points. By increasing the number of processors, slower than linear speedups are achieved with optimized (machine-dependent library) routines. This slower than linear speedup results because the computational cost is dominated by FFT routine, which yields less than ideal speedups. By using appropriate compile options and optimized library routines on the SP1, the serial code achieves 52-56 M ops on a single node of the SP1 (45 percent of theoretical peak performance). The actual performance of the PSDNS code on the SP1 is evaluated with a "real world" simulation that consists of 1.7 million grid points. One time step of this simulation is calculated on eight nodes of the SP1 in the same time as required by a Cray Y/MP supercomputer. For the same simulation, 32-nodes of the SP1 and SP2 are required to reach the performance of a Cray C-90. A 32 node SP1 (SP2) configuration is 2.9 (4.6) times faster than a Cray Y/MP for this simulation, while the hypercube is roughly 2 times slower than the Y/MP for this application. KEY WORDS: Spatial direct numerical simulations; incompressible viscous flows; spectral methods; finite differences; parallel computing.

Joslin, Ronald D.; Hanebutte, Ulf R.; Zubair, Mohammad

1995-01-01

155

The immunoregulatory roles of lung surfactant collectins SP-A, and SP-D, in allergen-induced airway inflammation.  

PubMed

It has become increasingly evident that pulmonary surfactant proteins, SP-A and SP-D, present in the alveolar and bronchial epithelial fluid linings, not only play significant functions in the innate defense mechanism against pathogens, but also are involved in immunomodulatory roles, which result in the protection against, and resolution of, allergen-induced airway inflammation. Studies on allergen-sensitized murine models, and asthmatic patients, show that SP-A and SP-D can: specifically bind to aero-allergens; inhibit mast cell degranulation and histamine release; and modulate the activation of alveolar macrophages and dendritic cells during the acute hypersensitive phase of allergic response. They also can alleviate chronic allergic inflammation by inhibiting T-lymphocyte proliferation as well as increasing phagocytosis of DNA fragments and clearance of apoptotic cell debris. Furthermore, it has emerged, from the studies on SP-D-deficient mice, that, when these mice are challenged with allergen, they develop increased eosinophil infiltration, and abnormal activation of lymphocytes, leading to the production of Th2 cytokines. Intranasal administration of SP-D significantly attenuated the asthmatic-like symptoms seen in allergen-sensitized wild-type, and SP-D-deficient, mice. These important findings provide a new insight of the role that surfactant proteins play in handling environmental stimuli and in their immunoregulation of airway inflammatory disease. PMID:17544824

Wang, Jiu-Yao; Reid, Kenneth B M

2007-01-01

156

Removal of Pb (II) by immobilized and free filaments of marine Oscillatoria sp. NTMS01 and Phormidium sp. NTMS02.  

PubMed

Pb(2+) removal ability of the immobilized and free filaments of marine cyanobacteria Oscillatoria sp. NTMS01 and Phormidium sp. NTMS02 was studied using batch experiments. Biosorption of lead by immobilized filaments was studied as a function of pH (2, 4, 6, 8, 10), contact time (5-180 min) and initial lead concentration (1, 3, 5, 7 mg/L) and the removal efficiency of free filaments was studied by culturing in the marine medium with the initial concentration (1, 3, 5, 7 mg/L) at pH 7 and incubated for 10 days. The maximum percentage removal was observed at 25 min for immobilized Oscillatoria sp. NTMS01 and 30 min for immobilized Phormidium sp. NTMS02. At 4th and 6th day of incubation, 89% and 77% removal was observed at 1 mg/L of initial lead concentration by free filaments of Oscillatoria sp. NTMS01 and Phormidium sp. NTMS02 respectively and further the removal was decreased with increasing concentration. Chlorophyll-a content was decreased in a dose dependent manner. About 40 and 50% reduction of chlorophyll-a was observed at higher concentration in Oscillatoria sp. NTMS01 and Phormidium sp. NTMS02 respectively. The adsorption capacity of immobilized Oscillatoria sp. NTMS01 was found to be (Q(max)) 217.39 which is comparatively higher than other sorbents. The Pb(2+) removal efficiency was performed as described in terms of Langmuir and Freundlich isotherms. These organisms is found to fit better by the Langmuir isotherms. PMID:21720797

Kumar, Muthukannan Satheesh; Rajeshwari, Kamaraj; Johnson, Shani; Thajuddin, Nooruddin; Gunasekaran, Muthukumaran

2011-09-01

157

Plant growth promoting properties of Halobacillus sp. and Halomonas sp. in presence of salinity and heavy metals.  

PubMed

Salinity and heavy metal stress are challenging problems in agriculture. Here we report the plant growth promoting ability of three moderate halophiles, Halobacillus sp. ADN1, Halomonas sp. MAN5, and Halobacillus sp. MAN6, in presence of both salinity and heavy metal stress. Halobacillus sp. ADN1, Halomonas sp. MAN5, and Halobacillus sp. MAN6 can tolerate 25, 21, and 29% NaCl, respectively and grow in presence of 1?mM cobalt, cadmium, and nickel and 0.04?mM mercury and 0.03?mM silver. Halobacillus sp. ADN1, Halomonas sp. MAN5, and Halobacillus sp. MAN6 produced 152.5, 95.3, and 167.3?µg/ml indole acetic acid (IAA) and could solubilize 61, 53, and 75 parts per million (ppm) phosphate, respectively in the presence of 15% NaCl. The production of IAA and solubilization of phosphate was well retained in the presence of salinity and heavy metals like 1?mM cadmium, 0.7?mM nickel, 0.04?mM mercury, and 0.03?mM silver. Besides, the strains showed amylase and protease activities and could produce hydrogen cyanide and ammonia in presence of salinity and heavy metals. A mixture of three strains enhanced the root growth of Sesuvium portulacastrum under saline and heavy metal stress, where the root length increased nearly 4.5?±?0.6 times and root dry weight increased 5.4?±?0.5 times as compared to control. These strains can thus be useful in microbial assisted phytoremediation of polluted saline soils. PMID:23775888

Desale, Prithviraj; Patel, Bhargav; Singh, Sukrit; Malhotra, Aakshi; Nawani, Neelu

2014-08-01

158

The sea urchin metallothionein system: Comparative evaluation of the SpMTA and SpMTB metal-binding preferences?  

PubMed Central

Metallothioneins (MTs) constitute a superfamily of ubiquitous metal-binding proteins of low molecular weight and high Cys content. They are involved in metal homeostasis and detoxification, amongst other proposed biological functions. Two MT isoforms (SpMTA and SpMTB) have been reported in the echinoderm Strongylocentrotus purpuratus (sea urchin), both containing 20 Cys residues and presenting extremely similar sequences, although showing distinct tissular and ontogenic expression patterns. Although exhaustive information is available for the Cd(II)-SpMTA complex, this including the full resolution of its 3D structure, no data has been reported concerning either SpMTA Zn(II) and Cu(I) binding properties, or the characterization of SpMTB at protein level. In this work, both the SpMTA and SpMTB isoforms, as well as their separate ? and ? domains, have been recombinantly synthesized in the presence of Zn(II), Cd(II) or Cu(II), and the corresponding metal complexes have been analyzed using electrospray mass spectrometry, and CD, ICP-AES and UV–vis spectroscopies. The results clearly show a better performance of isoform A when binding Zn(II) and Cd(II), and of isoform B when coordinating Cu(I). Thus, our results confirm the differential metal binding preference of SpMTA and SpMTB, which, together with the reported induction pattern of the respective genes, highlights how also in Echinodermata the MT polymorphism may be linked to the evolution of different physiological roles. PMID:23847757

Tomas, Mireia; Domènech, Jordi; Capdevila, Mercè; Bofill, Roger; Atrian, Sílvia

2013-01-01

159

Leifsonia kribbensis sp. nov., isolated from soil.  

PubMed

A yellow-pigmented actinobacterium, designated strain MSL-13T, was isolated from a soil sample collected from Bigeum Island, Republic of Korea, and its taxonomic position was determined by using a polyphasic approach. Strain MSL-13T showed phenotypic and chemotaxonomic properties consistent with its classification in the genus Leifsonia. 16S rRNA gene sequence analysis of strain MSL-13T with sequences from Leifsonia naganoensis DB103T, Leifsonia aquatica DSM 20146T, Leifsonia xyli subsp. cynodontis JCM 9733T, Leifsonia poae VKM Ac-1401T and Leifsonia shinshuensis DB102T revealed similarities of 96.22, 96.19, 95.77, 95.44 and 95.37 %, respectively, with differences of 39-65 nt among 1483 total nucleotides aligned. Based on differences in phenotypic and genotypic characteristics, strain MSL-13T (=KCTC 19267T =DSM 19272T) is designated as the type strain of a novel species of the genus Leifsonia, for which the name Leifsonia kribbensis sp. nov. is proposed. PMID:19126716

Dastager, Syed G; Lee, Jae-Chan; Ju, Yoon-Jung; Park, Dong-Jin; Kim, Chang-Jin

2009-01-01

160

Flaviramulus aquimarinus sp. nov., isolated from seawater.  

PubMed

A yellow-coloured, rod-shaped, non-motile, Gram-stain-negative and facultative anaerobic bacterium, designated KYW499(T), was isolated from seawater collected at Suncheon Bay, Republic of Korea. The isolate required sea salts for growth. Flexirubin-type pigments were absent. Major cellular fatty acids (>10?% of total) were iso-C15?:?0, iso-C15?:?1 G, iso-C17?:?0 3-OH, iso-C15?:?0 3-OH. The main polar lipids were phosphatidylethanolamine and two unidentified polar lipids. The predominant isoprenoid quinone was MK-6. The genomic DNA G+C content was 31.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KYW499(T) belonged to the family Flavobacteriaceae, and was related to the genus Flaviramulus. Data from a polyphasic taxonomy study suggested that the isolate represents a novel species of the genus Flaviramulus, for which the name Flaviramulus aquimarinus sp. nov. is proposed. The type strain is KYW499(T) (?=?KCTC 23924(T)?=?JCM 18274(T)). PMID:25413878

Lee, Ji Hee; Baik, Keun Sik; Seong, Chi Nam

2015-02-01

161

Pontibacter saemangeumensis sp. nov., isolated from seawater.  

PubMed

A gram-negative, rod-shaped, non-motile and pink bacterial strain, designated strain GCM0142(T), was isolated from the confined seawater in the Saemangeum Tide Embankment of South Korea, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis of strain GCM0142(T) indicated that the isolate belonged to the phylum Bacteroidetes and exhibited similarity levels of 94.0-96.4?% to the type strains of recognized Pontibacter species. Strain GCM0142(T) was oxidase- and catalase-positive. The major cellular fatty acids of the novel strain were summed feature 4 (comprising iso-C(17?:?1)I and/or anteiso-C(17?:?1)B, 36.8?%), iso-C(15?:?0) (22.3?%) and summed feature 3 (comprising C(16?:?1)?7c and/or C(16?:?1)?6c, 6.2?%). The DNA G+C content of strain GCM0142(T) was 48.9 mol% and the major quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids (AL1-2), an unknown aminophospholipid, five unknown lipids (L1-5) and an unknown glycolipid. On the basis of the evidence presented, strain GCM0142(T) is considered to represent a novel species of the genus Pontibacter, for which the name Pontibacter saemangeumensis sp. nov. is proposed. The type strain is GCM0142(T) (?=?KACC 16448(T)?=?JCM 17926(T)). PMID:22523168

Kang, Ji Young; Joung, Yochan; Chun, Jeesun; Kim, Haneul; Joh, Kiseong; Jahng, Kwang Yeop

2013-02-01

162

Gryllotalpicola soli sp. nov., isolated from soil.  

PubMed

A novel Gram-stain-positive, short rod-shaped, non-flagellated and mesophilic strain, KIS12-7(T), isolated from a soil sample collected from Daecheong-Island in Ongjin County, Republic of Korea, was studied using a polyphasic approach. Phylogenetic trees based on the 16S rRNA gene sequence revealed that the novel strain was a member of the genus Gryllotalpicola, showing more than 97.0?% sequence similarity with Gryllotalpicola daejeonensis RU-04(T) (98.0?%), Gryllotalpicola koreensis RU-16(T) (97.7?%) and Gryllotalpicola kribbensis PU-02(T) (97.3?%). However, DNA-DNA relatedness values demonstrated that strain KIS12-7(T) could be clearly distinguished from closely related species of the genus Gryllotalpicola. The cell-wall peptidoglycan of strain KIS12-7(T) was of the type B2 and the acyl type was acetyl. The predominant menaquinones were MK-11 and MK-10. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown phosphoglycolipid, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The G+C content of the genomic DNA was 72.1 mol%. On the basis of the evidence presented, strain KIS12-7(T) is a representative of a novel species of the genus Gryllotalpicola, and the name Gryllotalpicola soli sp. nov. is proposed; the type strain is KIS12-7(T) (?=?DSM 27182(T)?=?KACC 17302(T)?=?NBRC 109659(T)). PMID:25240021

Moon, Ji-Young; Kim, Soo-Jin; Hamada, Moriyuki; Ahn, Jae-Hyung; Weon, Hang-Yeon; Suzuki, Ken-Ichiro; Yoon, Jung-Hoon; Kwon, Soon-Wo

2014-12-01

163

Blastocystis sp. from food animals in India.  

PubMed

Blastocystis, a zoonotic protozoan found in the intestinal tracts of a wide range of animals, has not been reported from non-human hosts from India so far. Organisms indistinguishable from Blastocystis sp. were identified in the Giemsa stained intestinal scrapings collected from carcasses of piglet and poultry that were brought for necropsy to the Central University Laboratory, Chennai. The 'central vacuole forms' of the parasite, with number of nuclei ranging from 1 to 12 were identified. The intensity of infection was low, with less than one organism per oil immersion field, indicating that their presence was unconnected to the cause of death. Caecal scraping was found to be more ideal than duodenal scraping for the diagnosis of Blastocystis, and can be a potential specimen for definitive diagnosis. Identical organisms were also detected in the dung samples of a buffalo calf which showed clinical signs of diarrhoea The presence of Blastocystis in food animals acquires public health significance, as many subtypes of the parasite from poultry and pigs are transmissible to humans. PMID:25320500

Sreekumar, C; Selvaraj, J; Gomathinayagam, S; Thangapandiyan, M; Ravikumar, G; Roy, Parimal; Balachandran, C

2014-12-01

164

Physiological and morphological observations on Thiovulum sp.  

PubMed Central

Cell suspensions of Thiovulum sp., collected from enrichment cultures, were grown, maintained, and harvested for periods up to 7 months. In open-flow cultures run with aerated seawater, a continuous supply of hydrogen sulfide was provided by diffusion through a semipermeable membrane from either a live culture of Desulfovibrio esturaii, neutralized sodium sulfide, or a N2-H2S gas mixture. Attempts to grow Thiovulum in pure culture failed despite variation in concentrations of dissolved oxygen and hydrogen sulfide in stratified as well as in completely mixed systems. Uptake of 14CO2 and some organic compounds by purified cell suspensions was measured, and values were corrected for the activity of heterotrophic as well as autotrophic contaminants as determined in control experiments. Cell populations exhibited maximum uptake activities during formation of the characteristic veils. Substantial uptake of CO2 in air-saturated seawater was coincident with an optimal concentration of hydrogen sulfide of about 1 mM. Glutamate and a selection of vitamins (B12M biotin, and thiamine) did not significantly affect the uptake of CO2. No substantial uptake of carbon from acetate, glutamate, mannitol, and Casamino Acids was found. Within the range of error indicated, the data are consistent with acceptance of a chemolithotrophic nature of Thiovulum. Images PMID:101531

Wirsen, C O; Jannasch, H W

1978-01-01

165

Preliminary SP-100/Stirling heat exchanger designs  

NASA Technical Reports Server (NTRS)

Analytic modeling of several heat exchanger concepts to couple the SP-100 nuclear reactor primary lithium loop and the Space Stirling Power Convertor (SSPC) was performed. Four 25 kWe SSPC's are used to produce the required 100 kW of electrical power. This design work focused on the interface between a single SSPC and the primary lithium loop. Manifolding to separate and collect the four channel flow was not modeled. This work modeled two separate types of heat exchanger interfaces (conductive coupling and radiative coupling) to explore their relative advantages and disadvantages. The minimum mass design of the conductively coupled concepts was 18 kg or 0.73 kg/kWe for a single 25 kWe convertor. The minimum mass radiatively coupled concept was 41 kg or 1.64 kg/kWe. The direct conduction heat exchanger provides a lighter weight system because of its ability to operate the Stirling convertor evaporator at higher heat fluxes than those attainable by the radiatively coupled systems. Additionally the conductively coupled concepts had relatively small volumes and provide potentially simpler assembly. Their disadvantages were the tight tolerances and material joining problems associated with this refractory to superalloy interface. The advantages of the radiatively coupled designs were the minimal material interface problems.

Schmitz, Paul; Tower, Leonard; Dawson, Ronald; Blue, Brian; Dunn, Pat

1993-01-01

166

Amycolatopsis xylanica sp. nov., isolated from soil.  

PubMed

An actinomycete, designated CPCC 202699(T), was isolated from soil in Qinghai province, China, and its taxonomic status was established. Strain CPCC 202699(T) contained meso-diaminopimelic acid in its peptidoglycan, arabinose and galactose as the diagnostic sugars in whole-cell hydrolysates, a phospholipid pattern consisting mainly of phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylcholine, MK-9(H(4)) as the predominant menaquinone and iso-C(16 : 0) (18.8 %), iso-C(15 : 0) (18.1 %), iso-C(14 : 0) (14.2 %), C(16 : 1) cis9 (10.9 %) and C(17 : 1) cis9 (10.3 %) as the major fatty acids. In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 202699(T) formed a separate branch within the genus Amycolatopsis. However, strain CPCC 202699(T) showed low 16S rRNA gene sequence similarities (<97.0 %) with type strains of species with validly published names in the genus Amycolatopsis. On the basis of genotypic and phenotypic differences from the closest phylogenetic neighbours of strain CPCC 202699(T), a novel species, Amycolatopsis xylanica sp. nov., is proposed. The type strain is CPCC 202699(T) (=DSM 45285(T) =KCTC 19581(T) =CCM 7627(T)). PMID:19880636

Chen, Jie; Su, Jin-Jin; Wei, Yu-Zhen; Li, Qiu-Ping; Yu, Li-Yan; Liu, Hong-Yu; Zhang, Yu-Qin; Zhang, Yue-Qin

2010-09-01

167

Respiration patterns of resting wasps (Vespula sp.)  

PubMed Central

We investigated the respiration patterns of wasps (Vespula sp.) in their viable temperature range (2.9–42.4 °C) by measuring CO2 production and locomotor and endothermic activity. Wasps showed cycles of an interburst–burst type at low ambient temperatures (Ta < 5 °C) or typical discontinuous gas exchange patterns with closed, flutter and open phases. At high Ta of >31 °C, CO2 emission became cyclic. With rising Ta they enhanced CO2-emission primarily by an exponential increase in respiration frequency, from 2.6 mHz at 4.7 °C to 74 mHz at 39.7 °C. In the same range of Ta CO2 release per cycle decreased from 38.9 to 26.4 ?l g?1 cycle?1. A comparison of wasps with other insects showed that they are among the insects with a low respiratory frequency at a given resting metabolic rate (RMR), and a relatively flat increase of respiratory frequency with RMR. CO2 emission was always accompanied by abdominal respiration movements in all open phases and in 71.4% of the flutter phases, often accompanied by body movements. Results suggest that resting wasps gain their highly efficient gas exchange to a considerable extent via the length and type of respiration movements. PMID:23399474

Käfer, Helmut; Kovac, Helmut; Stabentheiner, Anton

2013-01-01

168

Detection of Plasmodium sp. in capybara.  

PubMed

In the present study, we have microscopically and molecularly surveyed blood samples from 11 captive capybaras (Hydrochaeris hydrochaeris) from the Sanctuary Zoo for Plasmodium sp. infection. One animal presented positive on blood smear by light microscopy. Polymerase chain reaction was carried out accordingly using a nested genus-specific protocol, which uses oligonucleotides from conserved sequences flanking a variable sequence region in the small subunit ribosomal RNA (ssrRNA) of all Plasmodium organisms. This revealed three positive animals. Products from two samples were purified and sequenced. The results showed less than 1% divergence between the two capybara sequences. When compared with GenBank sequences, a 55% similarity was obtained to Toxoplasma gondii and a higher similarity (73-77.2%) was found to ssrRNAs from Plasmodium species that infect reptile, avian, rodents, and human beings. The most similar Plasmodium sequence was from Plasmodium mexicanum that infects lizards of North America, where around 78% identity was found. This work is the first report of Plasmodium in capybaras, and due to the low similarity with other Plasmodium species, we suggest it is a new species, which, in the future could be denominated "Plasmodium hydrochaeri". PMID:19411142

dos Santos, Leonilda Correia; Curotto, Sandra Mara Rotter; de Moraes, Wanderlei; Cubas, Zalmir Silvino; Costa-Nascimento, Maria de Jesus; de Barros Filho, Ivan Roque; Biondo, Alexander Welker; Kirchgatter, Karin

2009-07-01

169

Floating assembly of diatom Coscinodiscus sp. microshells.  

PubMed

Diatoms have silica frustules with transparent and delicate micro/nano scale structures, two dimensional pore arrays, and large surface areas. Although, the diatom cells of Coscinodiscus sp. live underwater, we found that their valves can float on water and assemble together. Experiments show that the convex shape and the 40 nm sieve pores of the valves allow them to float on water, and that the buoyancy and the micro-range attractive forces cause the valves to assemble together at the highest point of water. As measured by AFM calibrated glass needles fixed in manipulator, the buoyancy force on a single floating valve may reach up to 10 ?N in water. Turning the valves over, enlarging the sieve pores, reducing the surface tension of water, or vacuum pumping may cause the floating valves to sink. After the water has evaporated, the floating valves remained in their assembled state and formed a monolayer film. The bonded diatom monolayer may be valuable in studies on diatom based optical devices, biosensors, solar cells, and batteries, to better use the optical and adsorption properties of frustules. The floating assembly phenomenon can also be used as a self-assembly method for fabricating monolayer of circular plates. PMID:22387476

Wang, Yu; Pan, Junfeng; Cai, Jun; Zhang, Deyuan

2012-03-30

170

Roseomonas aerophila sp. nov., isolated from air.  

PubMed

An aerobic, Gram-stain-negative, motile, mesophilic bacterium, designated strain 7515T-07(T), was isolated from an air sample in the Taean region, Republic of Korea. The strain grew at 4-40 °C (optimum, 30 °C) and pH 5.0-9.0 (optimum, pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 7515T-07(T) was related to members of the genus Roseomonas and had highest 16S rRNA gene sequence similarity with Roseomonas ludipueritiae 170/96(T) (96.7%). 16S RNA gene sequence similarity between strain 7515T-07(T) and Roseomonas gilardii ATCC 49956(T) (the type species of the genus Roseomonas) was 93.4%. Strain 7515T-07(T) contained Q-10 as the ubiquinone and C18:1?7c and C19:0 cyclo ?8c as the dominant fatty acids (>10%). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified aminolipids. The DNA G+C content was 73.0 mol%. Combined data from phenotypic, phylogenetic and chemotaxonomic studies demonstrated that strain 7515T-07(T) is a representative of a novel species of the genus Roseomonas, for which the name Roseomonas aerophila sp. nov. is proposed. The type strain is 7515T-07(T) (=KACC 16529(T)=NBRC 108923(T)). PMID:23178724

Kim, Soo-Jin; Weon, Hang-Yeon; Ahn, Jae-Hyung; Hong, Seung-Beom; Seok, Soon-Ja; Whang, Kyung-Sook; Kwon, Soon-Wo

2013-06-01

171

Chemoselective Activation of sp(3) vs sp(2) C-H Bonds with Pd(II).  

PubMed

The first selective coupling of a carbon nucleophile with methyl, ethyl, propyl, and butyl arenes in the absence of a directing group is described. Pd(OAc)2 double C-H activation displays remarkable selectivity for the terminal methyl sites in alkyl arenes, rather than the more commonly observed arene sp(2) C-H activation. Mechanistic studies indicate the intermediacy of an azlactone dimer, obtained from oxidation with Pd(OAc)2, and are consistent with a Pd-catalyzed C-H activation vs a radical process. The observed reactivity establishes that typical reaction solvents (e.g., toluene) can readily participate in C-H activation chemistry. PMID:25423164

Curto, John M; Kozlowski, Marisa C

2015-01-14

172

Geobacter anodireducens sp. nov., an exoelectrogenic microbe in bioelectrochemical  

E-print Network

Geobacter anodireducens sp. nov., an exoelectrogenic microbe in bioelectrochemical systems Dan Sun; Lovley & Phillips, 1986). Recently, it has been discovered that some of these microbes could produce systems (BESs) (Logan, 2008). These microbes, collectively called exoelectrogens, can be used

173

Users guide for the ANL IBM SP1  

SciTech Connect

This guide presents the features of the IBM SP1 installed in the Mathematics and Computer Science Division at Argonne National Laboratory. The guide describes the available hardware and software, access policies, and hints for using the system productively.

Gropp, W.; Lusk, E.; Pieper, S.C.

1994-10-01

174

PySP: Modeling and Solving Stochastic Programs in Python  

E-print Network

Sep 6, 2010 ... The modeling of uncertainty is widely recognized as an integral ...... penalize the lack of implementability using a sub-gradient estimator for the non- ...... in PySP or are under active investigation include biofuel network design ...

2010-09-07

175

Les ganodermes (Ganoderma sp.) Ganoderma adspersum (Schulz.) Donk  

E-print Network

Les ganodermes (Ganoderma sp.) Ganoderma adspersum (Schulz.) Donk Ordre : Ganodermales Famille conjonctives brunes à parois épaisses, r P induit une pou #12;Le ganoderme aplani Ganoderma lipsiense (Batsch

Chamroukhi, Faicel

176

Stimulation of bioluminescence in Noctiluca sp. using controlled temperature changes.  

PubMed

Bioluminescence induced by multifarious stimuli has long been observed and is remains under investigation because of its great complexity. In particular, the exact mechanism underlying bioluminescence is not yet fully understood. This work presents a new experimental method for studying Noctiluca sp. bioluminescence under temperature change stimulation. It is a study of Noctiluca sp. bioluminescence using controlled temperature changes in a tank. A characteristic of this experiment is the large volume of water used (1?m(3) in a tank of 2?×?1?×?1?m). Temperature changes were controlled by two methods. In the first, a flask filled with hot water was introduced into the tank and in the second, a water heater was used in the tank. Temperature changes were recorded using sensors. Noctiluca sp. bioluminescence was recorded using a Canon 5D Mark II and this allowed the characteristics of Noctiluca sp. bioluminescence under temperature change stimulation to be monitored. PMID:23001957

Han, Jing; Li, GuiJuan; Liu, HuanYing; Hu, HaoHao; Zhang, XueGang

2013-01-01

177

Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection  

PubMed Central

ABSTRACT Most DNA viruses associate with, and reorganize, nuclear domain 10 (ND10) bodies upon entry into the host nucleus. In this study, we examine the roles of the ND10 components PML, Sp100, and Daxx in the establishment of human papillomavirus type 18 (HPV18) infection of primary human keratinocytes. HPV18 DNA or HPV18 quasivirus was introduced into primary human keratinocytes depleted of each ND10 protein by small interfering RNA technology, and genome establishment was determined by using a quantitative immortalization assay and measurements of viral transcription and DNA replication. Keratinocyte depletion of Sp100 resulted in a substantial increase in the number of HPV18-immortalized colonies and a corresponding increase in viral transcription and DNA replication. However, Sp100 repressed viral transcription and replication only during the initial stages of viral establishment, suggesting that Sp100 acts as a repressor of incoming HPV DNA. PMID:24194542

Stepp, Wesley H.; Meyers, Jordan M.; McBride, Alison A.

2013-01-01

178

'PROCTOECES' SP. (TREMATODA: DIGENEA) IN THE AMERICAN OYSTER, 'CRASSOSTREA VIRGINICA'  

EPA Science Inventory

Histological examination of over 6,000 oysters, Crassostrea virginica, inhabiting northern Gulf Coast estuaries revealed unencysted junvenile and possible adult stages of digenetic trematode, Proctoeces sp., inhabiting the gonadal ducts of the mollusc. The morphology of the worm ...

179

Geobacter uraniireducens sp. nov., isolated from subsurface sediment undergoing uranium  

E-print Network

Geobacter uraniireducens sp. nov., isolated from subsurface sediment undergoing uranium with acetate oxidation, was isolated from subsurface sediment undergoing uranium bioremediation. The 16S r was to recover an environmentally relevant Geobacter strain from subsurface sediments undergoing in situ uranium

Lovley, Derek

180

Vanadium-Catalyzed C(sp3 )H Fluorination Reactions  

E-print Network

S1 Vanadium-Catalyzed C(sp3 )­H Fluorination Reactions Ji-Bao Xia, Yuyong Ma, and Chuo Chen. Vanadium(III) Oxide (95%) was purchased from Strem Chemicals Inc. Fluorobenzene (99.5%) was purchased from procedure for the V2O3-catalyzed C(sp3 )­H fluorination reaction. To a 4 mL clear vial charged with vanadium

Chen, Chuo

181

Sarcocystis sp. in the eastern cottontail (Sylvilagus floridanus).  

PubMed

Sarcocystis sp. was observed in 100 of 185 (54.1%) Eastern cottontails (Sylvilagus floridanus) examined in Pennsylvania over a three year period. Gross and histologic examination commonly revealed cysts in skeletal muscle of the fore and hind legs, flanks and loins. Two rabbits had cysts in esophageal skeletal muscle. Host response to Sarcocystis sp. is described. Adult rabbits had a significantly greater rate of infection (69.3%) than juveniles (20.7%) (P less than 0.01). PMID:6808158

Cosgrove, M; Wiggins, J P; Rothenbacher, H

1982-01-01

182

SP8 regulates signaling centers during craniofacial development  

PubMed Central

Much of the bone, cartilage and smooth muscle of the vertebrate face is derived from neural crest (NC) cells. During craniofacial development, the anterior neural ridge (ANR) and olfactory pit (OP) signaling centers are responsible for driving the outgrowth, survival, and differentiation of NC populated facial prominences, primarily via FGF. While much is known about the functional importance of signaling centers, relatively little is understood of how these signaling centers are made and maintained. In this report we describe a dramatic craniofacial malformation in mice mutant for the zinc finger transcription factor gene Sp8. At E14.5 they show facial prominences that are reduced in size and underdeveloped, giving an almost faceless phenotype. At later times they show severe midline defects, excencephaly, hyperterlorism, cleft palate, and a striking loss of many NC and paraxial mesoderm derived cranial bones. Sp8 expression was primarily restricted to the ANR and OP regions during craniofacial development. Analysis of an extensive series of conditional Sp8 mutants confirmed the critical role of Sp8 in signaling centers, and not directly in the NC and paraxial mesoderm cells. The NC cells of the Sp8 mutants showed increased levels of apoptosis and decreased cell proliferation, thereby explaining the reduced sizes of the facial prominences. Perturbed gene expression in the Sp8 mutants was examined by laser capture microdissection coupled with microarrays, as well as in situ hybridization and immunostaining. The most dramatic differences included striking reductions in Fgf8 and Fgf17 expression in the ANR and OP signaling centers. We were also able to achieve genetic and pharmaceutical partial rescue of the Sp8 mutant phenotype by reducing Sonic Hedgehog (SHH) signaling. These results show that Sp8 primarily functions to promote Fgf expression in the ANR and OP signaling centers that drive the survival, proliferation, and differentiation of the NC and paraxial mesoderm that make the face. PMID:23872235

Kasberg, Abigail D.; Brunskill, Eric W.; Potter, S. Steven

2014-01-01

183

Candida spencermartinsiae sp. nov., Candida taylorii sp. nov. and Pseudozyma abaconensis sp. nov., novel yeasts from mangrove and coral reef ecosystems.  

PubMed

Three species of yeasts are taxonomically described for strains isolated from marine environments. Candida spencermartinsiae sp. nov. (type strain CBS 10894T =NRRL Y-48663T) and Candida taylorii sp. nov. (type strain CBS 8508T =NRRL Y-27213T) are anamorphic ascomycetous yeasts in a phylogenetic cluster of marine yeasts in the Debaryomyces/Lodderomyces clade of the Saccharomycetales. The two species were isolated from multiple locations among coral reefs and mangrove habitats. Pseudozyma abaconensis sp. nov. (type strain CBS 8380T =NRRL Y-17380T) is an anamorphic basidiomycete that is related to the smut fungi of the genus Ustilago in the Ustilaginales. P. abaconensis was collected from waters adjacent to a coral reef. PMID:19783617

Statzell-Tallman, Adele; Scorzetti, Gloria; Fell, Jack W

2010-08-01

184

Morphologic and molecular characterization of new Cyclospora species from Ethiopian monkeys: C. cercopitheci sp.n., C. colobi sp.n., and C. papionis sp.n.  

PubMed Central

In recent years, human cyclosporiasis has emerged as an important infection, with large outbreaks in the United States and Canada. Understanding the biology and epidemiology of Cyclospora has been difficult and slow and has been complicated by not knowing the pathogen s origins, animal reservoirs (if any), and relationship to other coccidian parasites. This report provides morphologic and molecular characterization of three parasites isolated from primates and names each isolate: Cyclospora cercopitheci sp.n. for a species recovered from green monkeys, C. colobi sp.n. for a parasite from colobus monkeys, and C. papionis sp.n. for a species infecting baboons. These species, plus C. cayetanensis, which infects humans, increase to four the recognized species of Cyclospora infecting primates. These four species group homogeneously as a single branch intermediate between avian and mammalian Eimeria. Results of our analysis contribute toward clarification of the taxonomic position of Cyclospora and its relationship to other coccidian parasites. PMID:10511521

Eberhard, M. L.; da Silva, A. J.; Lilley, B. G.; Pieniazek, N. J.

1999-01-01

185

The astaxanthin dideoxyglycoside biosynthesis pathway in Sphingomonas sp. PB304.  

PubMed

A major carotenoid in Sphingomonas sp. PB304, originally isolated from a river in Daejon City, South Korea, was identified as astaxanthin dideoxyglycoside. Gene clusters encoding the astaxanthin dideoxyglycoside biosynthetic enzymes were identified by screening Sphingomonas sp. PB304 fosmid libraries using degenerate probes that harbor highly conserved sequences from the Sphigomonas elodea-derived crtI and Nostoc sp. PCC 7120-dervied crtW genes. Selected positive gene clusters were fully sequenced and annotated, revealing genes encoding six putative carotenogenic enzymes: phytoene synthase (CrtB), phytoene desaturase (CrtI), lycopene cyclase (CrtY), carotene hydroxylase (CrtZ), carotene ketolase (CrtW), and glycosyltransferase (CrtX). All of the carotenogenic enzymes, except for CrtX, were functional in the recombinant host Escherichia coli expressing synthetic carotenogenic modules from Pantoea agglomerans. CrtX did not take up UDP-glucose or GDP-fucose as sugar substrates during the in vitro reaction. Although no direct experimental evidence was obtained for the function of Sphingomonas sp. PB304 CrtX, it can be categorized as a putative deoxyglycosyltransferase based on the presence of astaxanthin dideoxyglycoside in Sphingomonas sp. PB304, a putative corresponding gene in the carotenoid biosynthetic gene cluster, and high amino acid sequence homology to the existing glycosyltransferases. Therefore, we propose that astaxanthin dideoxyglycoside can be synthesized in Sphingomonas sp. PB304 via sequential reactions of six pathway enzymes, including CrtX on the phytoene intermediate. PMID:25193422

Kim, Se Hyeuk; Kim, Jin Ho; Lee, Bun Yeol; Lee, Pyung Cheon

2014-12-01

186

Pin1-mediated Sp1 phosphorylation by CDK1 increases Sp1 stability and decreases its DNA-binding activity during mitosis  

PubMed Central

We have shown that Sp1 phosphorylation at Thr739 decreases its DNA-binding activity. In this study, we found that phosphorylation of Sp1 at Thr739 alone is necessary, but not sufficient for the inhibition of its DNA-binding activity during mitosis. We demonstrated that Pin1 could be recruited to the Thr739(p)-Pro motif of Sp1 to modulate the interaction between phospho-Sp1 and CDK1, thereby facilitating CDK1-mediated phosphorylation of Sp1 at Ser720, Thr723 and Thr737 during mitosis. Loss of the C-terminal end of Sp1 (amino acids 741-785) significantly increased Sp1 phosphorylation, implying that the C-terminus inhibits CDK1-mediated Sp1 phosphorylation. Binding analysis of Sp1 peptides to Pin1 by isothermal titration calorimetry indicated that Pin1 interacts with Thr739(p)-Sp1 peptide but not with Thr739-Sp1 peptide. X-ray crystallography data showed that the Thr739(p)-Sp1 peptide occupies the active site of Pin1. Increased Sp1 phosphorylation by CDK1 during mitosis not only stabilized Sp1 levels by decreasing interaction with ubiquitin E3-ligase RNF4 but also caused Sp1 to move out of the chromosomes completely by decreasing its DNA-binding activity, thereby facilitating cell cycle progression. Thus, Pin1-mediated conformational changes in the C-terminal region of Sp1 are critical for increased CDK1-mediated Sp1 phosphorylation to facilitate cell cycle progression during mitosis. PMID:25398907

Yang, Hang-Che; Chuang, Jian-Ying; Jeng, Wen-Yih; Liu, Chia-I; Wang, Andrew H.-J.; Lu, Pei-Jung; Chang, Wen-Chang; Hung, Jan-Jong

2014-01-01

187

Pin1-mediated Sp1 phosphorylation by CDK1 increases Sp1 stability and decreases its DNA-binding activity during mitosis.  

PubMed

We have shown that Sp1 phosphorylation at Thr739 decreases its DNA-binding activity. In this study, we found that phosphorylation of Sp1 at Thr739 alone is necessary, but not sufficient for the inhibition of its DNA-binding activity during mitosis. We demonstrated that Pin1 could be recruited to the Thr739(p)-Pro motif of Sp1 to modulate the interaction between phospho-Sp1 and CDK1, thereby facilitating CDK1-mediated phosphorylation of Sp1 at Ser720, Thr723 and Thr737 during mitosis. Loss of the C-terminal end of Sp1 (amino acids 741-785) significantly increased Sp1 phosphorylation, implying that the C-terminus inhibits CDK1-mediated Sp1 phosphorylation. Binding analysis of Sp1 peptides to Pin1 by isothermal titration calorimetry indicated that Pin1 interacts with Thr739(p)-Sp1 peptide but not with Thr739-Sp1 peptide. X-ray crystallography data showed that the Thr739(p)-Sp1 peptide occupies the active site of Pin1. Increased Sp1 phosphorylation by CDK1 during mitosis not only stabilized Sp1 levels by decreasing interaction with ubiquitin E3-ligase RNF4 but also caused Sp1 to move out of the chromosomes completely by decreasing its DNA-binding activity, thereby facilitating cell cycle progression. Thus, Pin1-mediated conformational changes in the C-terminal region of Sp1 are critical for increased CDK1-mediated Sp1 phosphorylation to facilitate cell cycle progression during mitosis. PMID:25398907

Yang, Hang-Che; Chuang, Jian-Ying; Jeng, Wen-Yih; Liu, Chia-I; Wang, Andrew H-J; Lu, Pei-Jung; Chang, Wen-Chang; Hung, Jan-Jong

2014-12-16

188

Evidence for cooperative mineralization of diuron by Arthrobacter sp. BS2 and Achromobacter sp. SP1 isolated from a mixed culture enriched from diuron exposed environments.  

PubMed

Diuron was found to be mineralized in buffer strip soil (BS) and in the sediments (SED) of the Morcille river in the Beaujolais vineyard repeatedly treated with this herbicide. Enrichment cultures from BS and SED samples led to the isolation of three bacterial strains transforming diuron to 3,4-dichloroaniline (3,4-DCA) its aniline derivative. 16S rRNA sequencing revealed that they belonged to the genus Arthrobacter (99% of similarity to Arthrobacter globiformis strain K01-01) and were designated as Arthrobacter sp. BS1, BS2 and SED1. Diuron-degrading potential characterized by sequencing of the puhA gene, characterizing the diuron-degradaing potential, revealed 99% similarity to A. globiformis strain D47 puhA gene isolated a decade ago in the UK. These isolates were also able to use chlorotoluron for their growth. Although able to degrade linuron and monolinuron to related aniline derivatives they were not growing on them. Enrichment cultures led to the isolation of a strain from the sediments entirely degrading 3,4-DCA. 16S rRNA sequence analysis showed that it was affiliated to the genus Achromobacter (99% of similarity to Achromobacter sp. CH1) and was designated as Achromobacter sp. SP1. The dcaQ gene encoding enzyme responsible for the transformation of 3,4-DCA to chlorocatechol was found in SP1 with 99% similarity to that of Comamonas testosteroni WDL7. This isolate also used for its growth a range of anilines (3-chloro-4-methyl-aniline, 4-isopropylaniline, 4-chloroaniline, 3-chloroaniline, 4-bromoaniline). The mixed culture composed of BS2 and SP1 strains entirely mineralizes (14)C-diuron to (14)CO2. Diuron-mineralization observed in the enrichment culture could result from the metabolic cooperation between these two populations. PMID:25061887

Devers-Lamrani, Marion; Pesce, Stéphane; Rouard, Nadine; Martin-Laurent, Fabrice

2014-12-01

189

Copper-catalyzed intramolecular C(sp³)-H and C(sp²)-H amidation by oxidative cyclization.  

PubMed

The first copper-catalyzed intramolecular C(sp(3))-H and C(sp(2))-H oxidative amidation has been developed. Using a Cu(OAc)2 catalyst and an Ag2CO3 oxidant in dichloroethane solvent, C(sp(3))-H amidation proceeded at a terminal methyl group, as well as at the internal benzylic position of an alkyl chain. This reaction has a broad substrate scope, and various ?-lactams were obtained in excellent yield, even on gram scale. Use of CuCl2 and Ag2CO3 under an O2 atmosphere in dimethyl sulfoxide, however, leads to 2-indolinone selectively by C(sp(2))-H amidation. Kinetic isotope effect (KIE) studies indicated that C-H bond activation is the rate-determining step. The 5-methoxyquinolyl directing group could be removed by oxidation. PMID:24616043

Wang, Zhen; Ni, Jizhi; Kuninobu, Yoichiro; Kanai, Motomu

2014-03-24

190

Streptococcus moroccensis sp. nov. and Streptococcus rifensis sp. nov., isolated from raw camel milk.  

PubMed

Two catalase- and oxidase-negative Streptococcus-like strains, LMG 27682(T) and LMG 27684(T), were isolated from raw camel milk in Morocco. Comparative 16S rRNA gene sequencing assigned these bacteria to the genus Streptococcus with Streptococcus rupicaprae 2777-2-07(T) as their closest phylogenetic neighbour (95.9% and 95.7% similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.7%. Although strains LMG 27682(T) and LMG 27684(T) shared a DNA-DNA hybridization value that corresponded to the threshold level for species delineation (68%), the two strains could be distinguished by multiple biochemical tests, sequence analysis of the phenylalanyl-tRNA synthase (pheS), RNA polymerase (rpoA) and ATP synthase (atpA) genes and by their MALDI-TOF MS profiles. On the basis of these considerable phenotypic and genotypic differences, we propose to classify both strains as novel species of the genus Streptococcus, for which the names Streptococcus moroccensis sp. nov. (type strain, LMG 27682(T) ?= CCMM B831(T)) and Streptococcus rifensis sp. nov. (type strain, LMG 27684(T) ?= CCMM B833(T)) are proposed. PMID:24786712

Kadri, Zaina; Amar, Mohamed; Ouadghiri, Mouna; Cnockaert, Margo; Aerts, Maarten; El Farricha, Omar; Vandamme, Peter

2014-07-01

191

Aminobacter ciceronei sp. nov. and Aminobacter lissarensis sp. nov., isolated from various terrestrial environments  

USGS Publications Warehouse

The bacterial strains IMB-1T and CC495T, which are capable of growth on methyl chloride (CH3Cl, chloromethane) and methyl bromide (CH3Br, bromomethane), were isolated from agricultural soil in California fumigated with CH3Br, and woodland soil in Northern Ireland, respectively. Two pesticide- /herbicide-degrading bacteria, strains ER2 and C147, were isolated from agricultural soil in Canada. Strain ER2 degrades N-methyl carbamate insecticides, and strain C147 degrades triazine herbicides widely used in agriculture. On the basis of their morphological, physiological and genotypic characteristics, these four strains are considered to represent two novel species of the genus Aminobacter, for which the names Aminobacter ciceronei sp. nov. (type strain IMB-1T=ATCC 202197T=CIP 108660T=CCUG 50580T; strains ER2 and C147) and Aminobacter lissarensis sp. nov. (type strain CC495T=NCIMB 13798T=CIP 108661T=CCUG 50579T) are proposed. ?? 2005 IUMS.

McDonald, I.R.; Kampfer, P.; Topp, E.; Warner, K.L.; Cox, M.J.; Connell, Hancock T.L.; Miller, L.G.; Larkin, M.J.; Ducrocq, V.; Coulter, C.; Harper, D.B.; Murrell, J.C.; Oremland, R.S.

2005-01-01

192

Serratia myotis sp. nov. and Serratia vespertilionis sp. nov., isolated from bats hibernating in caves.  

PubMed

During the study of bacteria associated with bats affected by white-nose syndrome hibernating in caves in the Czech Republic, we isolated two facultatively anaerobic, Gram-stain-negative bacteria, designated strains 12(T) and 52(T). Strains 12(T) and 52(T) were motile, rod-like bacteria (0.5-0.6 µm in diameter; 1-1.3 µm long), with optimal growth at 20-35 °C and pH 6-8. On the basis of the almost complete sequence of their 16S rRNA genes they should be classified within the genus Serratia; the closest relatives to strains 12(T) and 52(T) were Serratia quinivorans DSM 4597(T) (99.5?% similarity in 16S rRNA gene sequences) and Serratia ficaria DSM 4569(T) (99.5?% similarity in 16S rRNA gene sequences), respectively. DNA-DNA relatedness between strain 12(T) and S. quinivorans DSM 4597(T) was only 37.1?% and between strain 52(T) and S. ficaria DSM 4569(T) was only 56.2?%. Both values are far below the 70?% threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus Serratia as representatives of Serratia myotis sp. nov. (type strain 12(T)?=?CECT 8594(T)?=?DSM 28726(T)) and Serratia vespertilionis sp. nov. (type strain 52(T)?=?CECT 8595(T)?=?DSM 28727(T)). PMID:25281728

García-Fraile, P; Chudí?ková, M; Benada, O; Pikula, J; Kola?ík, M

2015-01-01

193

Streptococcus loxodontisalivarius sp. nov. and Streptococcus saliviloxodontae sp. nov., isolated from oral cavities of elephants.  

PubMed

Four Gram-stain-positive, catalase-negative, coccoid-shaped organisms were isolated from elephant oral cavities. The isolates were tentatively identified as streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene sequencing studies confirmed the organisms to be members of the genus Streptococcus. Two isolates (NUM 6304(T) and NUM 6312) were related most closely to Streptococcus salivarius with 96.8?% and 93.1?% similarity based on the 16S rRNA gene and the RNA polymerase ? subunit encoding gene (rpoB), respectively, and to Streptococcus vestibularis with 83.7?% similarity based on the 60 kDa heat-shock protein gene (groEL). The other two isolates (NUM 6306(T) and NUM 6318) were related most closely to S. vestibularis with 97.0?% and 82.9?% similarity based on the 16S rRNA and groEL genes, respectively, and to S. salivarius with 93.5?% similarity based on the rpoB gene. Based on phylogenetic and phenotypic evidence, these isolates are suggested to represent novel species of the genus Streptococcus, for which the names Streptococcus loxodontisalivarius sp. nov. (type strain NUM 6304(T)?=?JCM 19287(T)?=?DSM 27382(T)) and Streptococcus saliviloxodontae sp. nov. (type strain NUM 6306(T)?=?JCM 19288(T)?=?DSM 27513(T)) are proposed. PMID:24994777

Saito, Masanori; Shinozaki-Kuwahara, Noriko; Hirasawa, Masatomo; Takada, Kazuko

2014-09-01

194

Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.).  

PubMed

Four strains of aerobic, endospore-forming bacteria were isolated from the inner tissues of healthy cotton plants (Gossypium sp., Dushanbe, Tajikistan). The organisms had identical randomly amplified polymorphic DNA patterns that distinguished them from other bacilli that are commonly isolated from plant tissues, e.g. Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus pumilus and Bacillus subtilis. PCR amplification of 16S-23S rRNA spacer regions suggested that the four strains could be assigned to two highly related taxa, which correlated with differences in cell morphology. However, the cloned spacer region provided a simple and specific hybridization probe for all four strains. The virtually complete 16S rDNA sequences were prepared for representatives of the two groups (strains 2DT(T) and 12DX) and differed by only two bases, thus supporting classification of the four strains in a single taxon at the species level. Phylogenetic analyses indicated that strain 2DT(T) belonged to the genus Bacillus and was most closely related to Bacillus sporothermodurans DSM 10599T with a sequence similarity of 94.8%. It is concluded that the four strains belong to a novel species of Bacillus for which the name Bacillus endophyticus sp. nov. is proposed. The type strain is 2DT(T) (= UCM B-5715T = CIP 106778T). PMID:11837291

Reva, Oleg N; Smirnov, Valerie V; Pettersson, Bertil; Priest, Fergus G

2002-01-01

195

Pedobacter huanghensis sp. nov. and Pedobacter glacialis sp. nov., isolated from Arctic glacier foreland.  

PubMed

Two psychrotolerant, Gram-stain-negative, rod-shaped bacterial strains, designed M1-27(T) and 8-24(T), were subjected to polyphasic taxonomic studies. Strain M1-27(T) was isolated from the foreland of the Midtre Lovénbreen glacier, whereas strain 8-24(T) was isolated from the foreland of the Austre Lovénbreen glacier. Both were Arctic glacier forelands, near Ny-Ålesund, Svalbard Archipelago, Norway. Strains M1-27(T) and 8-24(T) exhibited 16S rRNA gene sequence similarities of 91.0-96.0% and 92.3-96.7%, respectively, to type strains of recognized species of the genus Pedobacter. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two strains were grouped with members of the genus Pedobacter, but represented distinct taxa. Both strains contained MK-7 as the predominant menaquinone. The DNA G+C contents of strains M1-27(T) and 8-24(T) were 43.8% and 39.4%, respectively. The phenotypic characteristics, biochemical properties and polygenetic analysis, clearly indicated that strains M1-27(T) (?=?CCTCC AB 2012936(T)?= LMG 28205(T)) and 8-24(T) (?= CCTCC AB 2012941(T)?= NRRL B-59993(T)) represent two novel species of the genus Pedobacter, for which the names Pedobacter huanghensis sp. nov. and Pedobacter glacialis sp. nov., respectively, are proposed. PMID:24763603

Qiu, Xia; Qu, Zhihao; Jiang, Fan; Ren, Lvzhi; Chang, Xulu; Kan, Wenjing; Fang, Chengxiang; Peng, Fang

2014-07-01

196

Paenibacillus pasadenensis sp. nov. and Paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility.  

PubMed

Two novel spore-forming, Gram-positive, mesophilic, heterotrophic bacteria representing two novel species were isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) at Pasadena, CA, USA. The incidence of similar strains was examined by screening the growing collection of isolates ( approximately 400 strains) obtained from the JPL-SAF using species-specific PCR primer sets designed from the 16S rRNA gene sequences of strains SAFN-016(T) and SAFN-007(T). Phylogenetic analysis of 16S rRNA gene sequences placed these novel isolates within the genus Paenibacillus. Two strains, SAFN-016(T) and SAFN-125, shared 98 % 16S rRNA gene sequence similarity with Paenibacillus timonensis and 97 % similarity with Paenibacillus macerans. Strain SAFN-007(T) showed 95.2 % 16S rRNA gene sequence similarity with Paenibacillus kobensis, its nearest phylogenetic neighbour. The results of DNA-DNA hybridization, physiological tests and biochemical analysis allowed genotypic and phenotypic differentiation of the isolates from currently recognized Paenibacillus species. Strain SAFN-007(T) and strains SAFN-016(T) and SAFN-125 are representatives of two separate novel species, for which the names Paenibacillus pasadenensis sp. nov. (type strain SAFN-007(T)=ATCC BAA-1211(T)=NBRC 101214(T)) and Paenibacillus barengoltzii sp. nov. (type strain SAFN-016(T)=ATCC BAA-1209(T)=NBRC 101215(T)) are proposed. PMID:16825621

Osman, Shariff; Satomi, Masataka; Venkateswaran, Kasthuri

2006-07-01

197

Characterization of a bioflocculant produced by a consortium of Halomonas sp. Okoh and Micrococcus sp. Leo.  

PubMed

The physicochemical and flocculating properties of a bioflocculant produced by a bacterial consortium composed of Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The purified bioflocculant was cation and pH dependent, and optimally flocculated kaolin clay suspension at a dosage of 0.1 mg/mL. The flocculating activity of the bioflocculant was stimulated in the presence of Ca2+, Mn2+, Al3+ and had a wide pH range of 2-10, with the highest flocculating activity of 86% at pH 8. The bioflocculant was thermostable and retained more than 70% of its flocculating activity after being heated at 80 °C for 30 min. Thermogravimetric analyses revealed a partial thermal decomposition of the biofloculant at 400 °C. The infrared spectrum showed the presence of hydroxyl, carboxyl and amino moieties as functional groups. The bioflocculant produced by the bacterial consortium appears to hold promising alternative to inorganic and synthetic organic flocculants that are widely used in wastewater treatment. PMID:24135818

Okaiyeto, Kunle; Nwodo, Uchechukwu U; Mabinya, Leonard V; Okoh, Anthony I

2013-10-01

198

Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces.  

PubMed

Six strains (CB7(T), CB18, CB23, CB26, CB28 and CB35(T)) were isolated from human faeces. Based on phylogenetic analysis, phenotypic characteristics, cellular fatty acid profiles and menaquinone profiles, these strains could be included within the genus Prevotella and made up two clusters. 16S rRNA gene sequence analysis indicated that five strains were most closely related to Prevotella veroralis, sharing about 92 % sequence similarity; the remaining strain was most closely related to Prevotella shahii, sharing about 90 % sequence similarity. All six strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. The cellular fatty acid compositions of the six strains differed significantly from those of other Prevotella species. Five strains (CB7(T), CB18, CB23, CB26 and CB28) contained dimethyl acetals and the major menaquinones of these strains were MK-11, MK-12 and MK-13. The major menaquinones of CB35(T) were MK-12 and MK-13. Based on phenotypic and phylogenetic findings, two novel species, Prevotella copri sp. nov. and Prevotella stercorea sp. nov., are proposed, representing the two different strain clusters. The DNA G+C contents of strains CB7(T) and CB35(T) were 45.3 and 48.2 mol%, respectively. The type strains of P. copri and P. stercorea are CB7(T) (=JCM 13464(T)=DSM 18205(T)) and CB35(T) (=JCM 13469(T)=DSM 18206(T)), respectively. PMID:17473237

Hayashi, Hidenori; Shibata, Kensaku; Sakamoto, Mitsuo; Tomita, Shinichi; Benno, Yoshimi

2007-05-01

199

Streptomyces zagrosensis sp. nov., isolated from soil.  

PubMed

The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus Streptomyces. On ISP2 medium, strain HM 1154(T) produced a dark cream, branched substrate mycelium and Retinaculiaperti aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 °C and pH 6-9 with 0-4% (w/v) NaCl. Whole-cell hydrolysates contained ll-diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H4) and MK-9(H2) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C16:0 and anteiso-C15:0. Strain HM 1154(T) exhibited the highest 16S rRNA gene sequence similarities to Streptomyces coerulescens DSM 40146(T) (99.4%), Streptomyces varsoviensis DSM 40346(T) (99.3%), Streptomyces youssoufiensis DSM 41920(T) (99.2%), Streptomyces abikoensis DSM 40831(T) (99.2%), Streptomyces rimosus subsp. rimosus DSM 40260(T) (99.1%), Streptomyces luteireticuli DSM 40509(T) (99.1%), Streptomyces thioluteus DSM 40027(T) (99.1%), Streptomyces blastmyceticus DSM 40029(T) (99.0%) and Streptomyces hiroshimensis DSM 40037(T) (99.0%). DNA-DNA hybridization studies showed relatedness values of 11.0-35.8% with the closest related species. Based on these results, strain HM 1154(T) is considered to represent a novel species within the genus Streptomyces, for which the name Streptomyces zagrosensis sp. nov. is proposed. The type strain is HM 1154(T) ( = DSM 42018(T) = UTMC 1154(T) = CECT 8305(T)). PMID:25030519

Mohammadipanah, Fatemeh; Hamedi, Javad; Spröer, Cathrin; Rohde, Manfred; Montero-Calasanz, María del Carmen; Klenk, Hans-Peter

2014-10-01

200

Comamonas humi sp. nov., isolated from soil.  

PubMed

A bacterial strain, designated GAU11(T), was isolated from soil in Japan. Cells of the strain were Gram-stain-negative, aerobic, non-motile rods. The 16S rRNA gene sequence of strain GAU11(T) showed high similarity to those of Comamonas zonglianii BF-3(T) (98.8?%), Pseudacidovorax intermedius CC21(T) (96.4?%), Acidovorax caeni R-24608(T) (96.2?%), Alicycliphilus denitrificans K601(T) (96.2?%), Pseudorhodoferax soli TBEA3(T) (95.9?%) and Comamonas terrigena LMG 1253(T) (95.9?%). Strain GAU11(T) contained ubiquinone 8 as the sole ubiquinone and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Its major cellular fatty acids were C16?:?0, C18?:?1?7c and summed feature 3 (C16?:?1?7c and/or iso-C15?:?0 2-OH). The DNA G+C content of strain GAU11(T) was 68.2 mol%. The DNA-DNA relatedness between strain GAU11(T) and C. zonglianii DSM 22523(T) was 52 or 68?% (reciprocal value). Phenotypic characterization indicated that strain GAU11(T) represents a member of the genus Comamonas, but at the same time distinguished it from C. zonglianii DSM 22523(T). From polyphasic characterization, this strain should be classified as representing a novel species of the genus Comamonas, for which the name Comamonas humi sp. nov. (type strain GAU11(T)?=?JCM 19903(T)?=?DSM 28451(T)) is proposed. PMID:25212224

Hatayama, Kouta

2014-12-01

201

Streptomyces seymenliensis sp. nov., isolated from soil.  

PubMed

A novel actinomycete, designated strain B1041(T), isolated from soil collected from the Tuz (Salt) Lake in the central Anatolia region, in Turkey, was characterised using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the members of the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree. Strain B1041(T) was found to be most closely related to Streptomyces plumbiresistens CCNWHX 13-160(T) (98.46 % sequence similarity), Streptomyces pseudovenezuelae NBRC 12904(T) (97.81 %), Streptomyces novaecaesareae NBRC 13368(T) (97.68 %), Streptomyces graminifolii JL-22(T) (97.60 %), Streptomyces phaeoluteigriseus NRRL ISP-5182(T) (97.58 %), Streptomyces ciscaucasicus NBRC 12872(T) (97.53 %) and Streptomyces pratensis ch24(T) (97.52 %). Sequence similarities with other strains of the genus Streptomyces were lower than 97.5 %. The cell wall of the novel strain was found to contain LL-diaminopimelic acid and the whole cell sugars were identified as galactose, glucose and ribose. The major cellular fatty acids were identified as anteiso C15:0 , iso C16:0 and anteiso C17:0. The predominant menaquinones found were found to be MK-9(H8) and MK-9(H6). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, a phosphoglycolipid and two unknown phospholipids. The genomic DNA G+C content was determined to be 70.4 mol%. On the basis of the data from this polyphasic taxonomic study, strain B1041(T) can be considered to represent a novel species within the genus Streptomyces for which the name Streptomyces seymenliensis sp. nov. is proposed (type strain B1041(T) =KCTC 29245(T) = DSM 42117(T)). PMID:25424909

Tatar, Demet; Sahin, Nevzat

2015-02-01

202

Shewanella dokdonensis sp. nov., isolated from seawater.  

PubMed

A novel bacterial strain, designated UDC329(T), was isolated from a sample of seawater collected at Dong-do, on the coast of Dokdo Island, in the East Sea of the Republic of Korea. The Gram-staining-negative, motile, facultatively anaerobic, non-spore-forming rods of the strain developed into dark orange-yellow colonies. The strain grew optimally between 25 and 30 °C, with 1% (w/v) NaCl and at pH 7. It grew in the absence of NaCl, but not with NaCl at >7% (w/v). The predominant menaquinone was MK-7, the predominant ubiquinones were Q-7 and Q-8, and the major fatty acids were iso-C(15:0) (33.52%) and C(17:1)?8c (11.73%). The genomic DNA G+C content of strain UDC329(T) was 50.2 mol%. In phylogenetic analyses based on 16S rRNA and gyrB gene sequences, strain UDC329(T) was grouped with members of the genus Shewanella and appeared most closely related to Shewanella fodinae JC15(T) (97.9% 16S rRNA gene sequence similarity), Shewanella indica KJW27(T) (95.0%), Shewanella algae ATCC 51192(T) (94.8%), Shewanella haliotis DW01(T) (94.5%) and Shewanella chilikensis JC5(T) (93.9%). The level of DNA-DNA relatedness between strain UDC329(T) and S. fodinae JC15(T) was, however, only 27.4%. On the basis of phenotypic, genotypic and DNA-DNA relatedness data, strain UDC329(T) represents a novel species in the genus Shewanella, for which the name Shewanella dokdonensis sp. nov. is proposed. The type strain is UDC329(T) (=KCTC 22898(T)=DSM 23626(T)). PMID:21890724

Sung, Hye-Ri; Yoon, Jung-Hoon; Ghim, Sa-Youl

2012-07-01

203

Planomicrobium soli sp. nov., isolated from soil.  

PubMed

A Gram-staining-positive bacterium, designated strain XN13(T), was isolated from a soil sample collected from ALaShan National Geological Park in Inner Mongolia Autonomous Region, China and subjected to a taxonomic study using a polyphasic approach. Strain XN13(T) was found to have a range of chemical and morphological properties consistent with its classification in the genus Planomicrobium. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain XN13(T) was related to members of the genus Planomicrobium. The closest phylogenetic relatives were Planomicrobium okeanokoites NBRC 12536(T), Planomicrobium koreense JG07(T), Planomicrobium mcmeekinii S23F2(T) and Planomicrobium flavidum ISL-41(T) with 98.2%, 97.8%, 97.8% and 97.7% 16S rRNA gene sequence similarity, respectively. The major cellular fatty acids were anteiso-C(15?:?0), C(16?:?1)?7c alcohol, iso-C(14?:?0) and C(16?:?1)?11c. The predominant menaquinones were MK-8 and MK-7. The DNA G+C content was 40.3 mol%. The DNA-DNA relatedness values between strain XN13(T) and Planomicrobium okeanokoites KCTC 3672(T), Planomicrobium koreense KCTC 3684(T), P. mcmeekinii CGMCC 1.2724(T), Planomicrobium flavidum KCTC 13261(T), Planomicrobium chinense CGMCC 1.3454(T) and Planomicrobium glaciei CGMCC 1.6846(T) were 36%, 30%, 34%, 29%, 30% and 31%, respectively. The organism is different from recognized species of the genus Planomicrobium in several phenotypic characteristics. On the basis of phenotypic and genotypic properties, strain XN13(T) represents a novel species of the genus Planomicrobium, for which the name Planomicrobium soli sp. nov. is proposed. The type strain is XN13(T) (?=?CGMCC 1.12259(T)?=?KCTC 33047(T)). PMID:24854007

Luo, Xiaonan; Zhang, Jianli; Li, Dai; Xin, Yuhua; Xin, Di; Fan, Lei

2014-08-01

204

Flavobacterium maotaiense sp. nov., isolated from freshwater.  

PubMed

Two novel strains, T9(T) and T10, were isolated from water samples collected from Chishui River flowing through Maotai town, Guizhou, south-west China. The isolates were yellow-pigmented, Gram-reaction-negative, rod-shaped, non-motile and aerobic. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates belonged to the genus Flavobacterium, and showed highest similarities to Flavobacterium hibernum DSM 12611(T) (97.0?%), followed by Flavobacterium granuli Kw05(T) (96.7?%) and Flavobacterium pectinovorum DSM 6368(T) (96.7?%). The novel strains were able to grow at 20-37 °C (optimum 25 °C), pH 7.0-10.0 (optimum pH 7.0-8.0) and with 0-0.5?% (w/v) NaCl (optimum 0.5?%). The predominant fatty acids were iso-C15?:?0, C16?:?1?7c, anteiso-C15?:?0, C15?:?0, iso-C15?:?0 3-OH and iso-C15?:?1?10c, and menaquinone-6 (MK-6) was the main respiratory quinone. The major polar lipids were phosphatidylethanolamine, one unknown glycolipid, two unknown aminolipids and two unidentified lipids. The DNA G+C contents of strains T9(T) and T10 were 37.7 and 36.4 mol%, respectively. According to the phenotypic and genetic data, strains T9(T) and T10 represent a novel species in the genus Flavobacterium, for which the name Flavobacterium maotaiense sp. nov. is proposed. The type strain is T9(T) (?=?CGMCC 1.12712(T)?=?JCM 19927(T)). PMID:25313092

Feng, Qingqing; Gao, Yuan; Nogi, Yuichi; Tan, Xu; Han, Lu; Zhang, Yali; Lv, Jie

2015-01-01

205

Streptomyces burgazadensis sp. nov., isolated from soil.  

PubMed

A novel actinobacterial strain, designated Z1R7(T), was isolated from a soil sample collected from Burgazada, in the Marmara Sea (Turkey), and the strain identity was determined using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and it formed a distinct phyletic line in the 16S rRNA gene tree, together with the type strains Streptomyces specialis GW41-1564(T) (95.76?%), Streptomyces mayteni YIM 60475(T) (95.64?%), Streptomyces hainanensis YIM 47672(T) (95.53?%), Streptomyces hoynatensis S1412(T) (95.29?%), Streptomyces avicenniae MCCC 1A01535(T) (94.74?%), Streptomyces sedi YIM 65188(T) (94.59?%) and Streptomyces zhaozhouensis NEAU-LZS-5(T) (94.68?%). Chomotaxonomic data revealed that strain Z1R7(T) possesed MK-9 (H8) as the predominant menaquinone, ll-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose and ribose as whole cell sugars. Diphosphatidylglycerol, phoshphatidylethanolamine and phosphatidylinositol were the predominant polar lipids; iso-C16?:?0, anteiso-C17?:?0 and anteiso-C15?:?0 were the major fatty acids, and the genomic DNA G+C content was 69.4 mol%. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1R7(T) (?=?KCTC 29434(T)?=?DSM 42126(T)) should be classified in the genus Streptomyces as Streptomyces burgazadensis sp. nov. PMID:25237147

Saricaoglu, Salih; Isik, Kamil; Veyisoglu, Aysel; Saygin, Hayrettin; Cetin, Demet; Guven, Kiymet; Spröer, Cathrin; Klenk, Hans-Peter; Sahin, Nevzat

2014-12-01

206

Acinetobacter kookii sp. nov., isolated from soil.  

PubMed

Two Gram-stain-negative, non-fermentative bacterial strains, designated 11-0202(T) and 11-0607, were isolated from soil in South Korea, and four others, LUH 13522, LUH 8638, LUH 10268 and LUH 10288, were isolated from a beet field in Germany, soil in the Netherlands, and sediment of integrated fish farms in Malaysia and Thailand, respectively. Based on 16S rRNA, rpoB and gyrB gene sequences, they are considered to represent a novel species of the genus Acinetobacter. Their 16S rRNA gene sequences showed greatest pairwise similarity to Acinetobacter beijerinckii NIPH 838(T) (97.9-98.4?%). They shared highest rpoB and gyrB gene sequence similarity with Acinetobacter johnsonii DSM 6963(T) and Acinetobacter bouvetii 4B02(T) (85.4-87.6 and 78.1-82.7?%, respectively). Strain 11-0202(T) displayed low DNA-DNA reassociation values (<40?%) with the most closely related species of the genus Acinetobacter. The six strains utilized azelate, 2,3-butanediol, ethanol and dl-lactate as sole carbon sources. Cellular fatty acid analyses showed similarities to profiles of related species of the genus Acinetobacter: summed feature 3 (C16?:?1?7c, C16?:?1?6c; 24.3-27.2?%), C18?:?1?9c (19.9-22.1?%), C16?:?0 (15.2-22.0?%) and C12?:?0 (9.2-14.2?%). On the basis of the current findings, it is concluded that the six strains represent a novel species, for which the name Acinetobacter kookii sp. nov. is proposed. The type strain is 11-0202(T) (?=?KCTC 32033(T)?=?JCM 18512(T)). PMID:23950148

Choi, Ji Young; Ko, Gwangpyo; Jheong, Weonghwa; Huys, Geert; Seifert, Harald; Dijkshoorn, Lenie; Ko, Kwan Soo

2013-12-01

207

Hymenobacter roseus sp. nov., isolated from sand.  

PubMed

Strain JC245(T) was isolated from a sand sample, and appeared as dark pink colonies on agar plates with cells staining Gram-negative. Catalase and oxidase activities were positive. Casein was hydrolysed while chitin, gelatin and starch were not. Major (>5?%) fatty acids were iso-C15?:?0, iso-C15?:?0 3-OH, C16?:?1?5c, C16?:?1?6c/C16?:?1?7c, anteiso-C17?:?1 B/iso-C17?:?1 I and iso-C17?:?0 3-OH. Strain JC245(T) contained phosphatidylethanolamine and two unidentified lipids as the major polar lipids, with minor amounts of four unidentified lipids and an unidentified amino lipid. Bacterial hopane derivatives and adenosylhopane were the major hopanoids. Hydroxyflexixanthin was identified as one of the major carotenoids of strain JC245(T) along with five unidentified carotenoids. The genomic DNA G+C content was 52.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC245(T) represents a member of the genus Hymenobacter within the family Cytophagaceae of the phylum Bacteroidetes. Strain JC245(T) shared the highest 16S rRNA gene sequence similarity with Hymenobacter roseosalivarius AA-718(T) (98.3?%) and other members of the genus Hymenobacter (<95.1?%). However, strain JC245(T) showed 21±2?% relatedness (based on DNA-DNA hybridization) with H. roseosalivarius DSM 11622(T). Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC245(T) as a representative of a novel species in the genus Hymenobacter, for which the name Hymenobacter roseus sp. nov. is proposed. The type strain is JC245(T) (?=?KCTC 42090(T)?=?LMG 28260(T)). PMID:25242537

Subhash, Y; Sasikala, Ch; Ramana, Ch V

2014-12-01

208

Nocardioides daecheongensis sp. nov., isolated from soil.  

PubMed

Strain KIS2-16(T) was isolated from a soil sample collected from Daecheong Island of Incheon region, South Korea. KIS2-16(T) was Gram-staining-positive, aerobic, non-spore-forming, non-motile, catalase-positive, oxidase-negative and mesophilic. On the basis of 16S rRNA gene sequence analysis, strain KIS2-16(T) represented a member of the genus Nocardioides, being most closely related to the type strains of species of the genus Nocardioides, Nocardioides maradonensis RP-B30(T) (97.8?% sequence similarity) and Nocardioides ultimimeridianus RP-B26(T) (97.0?%). The fatty acid profile of KIS2-16(T) was dominated by C18?:?1?9c, C17?:?1?8c, C16?:?0, C18?:?0 10-methyl (TBSA), C16?:?0 2-OH and C17?:?0 2-OH. The major isoprenoid quinone was MK-8(H4), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The peptidoglycan structure was A3?-type with ll-diaminopimelic acid. The genomic DNA G+C content of KIS2-16(T) was 64.9 mol%. Strain KIS2-16(T) showed DNA-DNA hybridization values of less than 70?% with the closely related species of the genus Nocardioides. Based on phenotypic, genotypic and phylogenetic data, the isolate represents a novel species of the genus Nocardioides, for which the name Nocardioides daecheongensis sp. nov. (type strain KIS2-16(T)?=?DSM 27136(T)?=?KACC 17297(T)?=?NBRC 109597(T)) is proposed. PMID:25249564

Lim, Jun-Muk; Kim, Soo-Jin; Hamada, Moriyuki; Ahn, Jae-Hyung; Weon, Hang-Yeon; Suzuki, Ken-Ichiro; Ahn, Tae-Young; Kwon, Soon-Wo

2014-12-01

209

Paenibacillus tianmuensis sp. nov., isolated from soil.  

PubMed

Two closely related, Gram-stain-negative, rod-shaped, spore-forming strains, B27(T) and F6-B70, were isolated from soil samples of Tianmu Mountain National Natural Reserve in Zhejiang, China. Phylogenetic analysis based on 16S rRNA gene and rpoB sequences indicated that the isolates were members of the genus Paenibacillus. Both isolates were closely related to Paenibacillus ehimensis IFO 15659(T), Paenibacillus elgii SD17(T) and Paenibacillus koreensis YC300(T) (? 95.2 % 16S rRNA gene sequence similarity). DNA-DNA relatedness between strain B27(T) and P. ehimensis DSM 11029(T), P. elgii NBRC 100335(T) and P. koreensis KCTC 2393(T) was 21.2, 28.6 and 16.8 %, respectively. The major cellular fatty acids of strains B27(T) and F6-B70 were anteiso-C(15 : 0) and iso-C(15 : 0). The cell wall contained meso-diaminopimelic acid. The two isolates differed from their closest neighbours in terms of phenotypic characteristics and cellular fatty acid profiles (such as variable for oxidase, negative for methyl red test, unable to produce acid from d-fructose and glycogen and relatively higher amounts of iso-C(15 : 0) and lower amounts of C(16 : 0) and iso-C(16 : 0)). Strains B27(T) and F6-B70 represent a novel species of the genus Paenibacillus, for which the name Paenibacillus tianmuensis sp. nov. is proposed. The type strain is B27(T) (?=?DSM 22342(T) ?=?CGMCC 1.8946(T)). PMID:20543152

Wu, Xuechang; Fang, Haihuan; Qian, Chaodong; Wen, Yanping; Shen, Xiaobo; Li, Ou; Gao, Haichun

2011-05-01

210

Genetically confirmed Fasciola hepatogigantica n.sp.  

PubMed

Identification of liver fluke species cannot be achieved by clinical, pathological, coprological or immunological methods. However, the differential diagnosis between F. hepatica and F. gigantica infection is very important because of their different pathological manifestations. Moreover, in countries where the two species co-exist, morphologically intermediate forms were reported. The present study aimed to identify these forms by the use of molecular characterization of DNA sequence. Based on morphometric criteria, adults of Fasciola hepatica, F. gigantica and intermediate forms were collected from naturally infected sheep and cattle from various regions of Sohag Governorate. A simple and rapid new method (QIAamp DNA Mini Kit) was used to isolate DNA from the worms and their RELP patterns were obtained after digestion of the PCR products with AvalI restriction enzymes. The result of a regular PCR experiment for the amplification of the selected 28S rDNA fragment with the designed primer set yielded identical 618- bp-long PCR products for the three types of Fasciola where the RFLP profile obtained from F. hepatica revealed four fragments of 628, 575, 165 and 95 bp, and F. gigantica generated three fragments corresponding to 628, 358 and 300 bp fragments whereas the intermediate forms revealed four fragments of 628, 541, 358 and 300 bp, which were similar to those ofF. gigantica but with a distinctive fragment of 541. These results confirmed that three species are present in our locality: F. hepatica, F. gigantica and an intermediate form which was named F. hepatogigantica n.sp. on basis of having few morphometric characters from F. hepatica (length and pattern of uterine coils) but genetically they were more related to F. gigantica. PMID:23697013

Khalifa, Refaat M A; El-Hady, Hanaa A; Omran, Eman K; Ahmed, Noha S

2013-04-01

211

Cellulosimicrobium terreum sp. nov., isolated from soil.  

PubMed

A Gram-positive, yellow-pigmented, non-motile and rod-shaped or coccoid bacterial strain, DS-61T, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. The strain grew optimally at pH 6.5-7.5 and 25 degrees C in the presence of 1.0% (w/v) NaCl. Strain DS-61T had peptidoglycan of the type based on L-Lys-L-Thr-D-Asp and contained galactose as the only whole-cell sugar. MK-9(H4) was the predominant menaquinone and anteiso-C15:0 and iso-C15:0 were the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The DNA G+C content was 72.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-61T is most closely affiliated to the genus Cellulosimicrobium, clustering with Cellulosimicrobium cellulans and Cellulosimicrobium funkei. The levels of 16S rRNA gene sequence similarity between strain DS-61T and the type strains of Cellulosimicrobium cellulans and Cellulosimicrobium funkei were 97.4-97.6%. DNA-DNA relatedness data and differential phenotypic properties demonstrated that strain DS-61T is distinguishable from these two recognized Cellulosimicrobium species. On the basis of phenotypic, phylogenetic and genetic data, strain DS-61T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium terreum sp. nov. is proposed. The type strain is DS-61T (=KCTC 19206T=DSM 18665T). An emended description of the genus is given. PMID:17978207

Yoon, Jung-Hoon; Kang, So-Jung; Schumann, Peter; Oh, Tae-Kwang

2007-11-01

212

Nitrimines as Reagents for Metal-Free Formal C(sp(2) )-C(sp(2) ) Cross-Coupling Reactions.  

PubMed

Nitrimines are employed as powerful reagents for metal-free formal C(sp(2) )-C(sp(2) ) cross-coupling reactions. The new chemical process is tolerant of a wide array of nitrimine and heterocyclic coupling partners giving rise to the corresponding di- or trisubstituted alkenes, typically in high yield and with high stereoselectivity. This method is ideal for the metal-free construction of heterocycle-containing drug targets, such as phenprocoumon. PMID:25365926

Angeles-Dunham, Veronica V; Nickerson, David M; Ray, Devin M; Mattson, Anita E

2014-12-22

213

Endosymbiosis Formation Between a Nitrogen-Fixing Mix of Bacteria ( Xanthomonas sp. + Arthrobacter sp. ) and Wheat Root Cells  

Microsoft Academic Search

This research attempted to produce para-nodules on wheat roots and to study them by SEM and TEM. The auxin-like 2,4-D (at\\u000a 0.1 ?\\/mL) was used as an abiological nodulation agent. A nitrogen-fixing mixed culture (Arthrobacter sp + Xanthomonas sp) was used to inoculate wheat (Triticum aestivum var. Pameiti Fedina) seedlings that were isolated from typical Sierozem in Turkmen. SEM showed

B. Abbas

214

Description of Sarcocystis anasi sp. nov. and Sarcocystis albifronsi sp. nov. in birds of the order Anseriformes  

Microsoft Academic Search

On the basis of the already published morphological, 18S rDNA, 28S rDNA data (Kutkien? et al., Parasitol Res 99:562–565, 2006; Parasitol Res 102:691–696, 2008; Parasitol Res 104:329–336, 2009), and ITS-1 region investigation results of sarcocysts presented in this paper, Sarcocystis albifronsi sp. nov. from the white-fronted goose (Anser albifrons) and Sarcocystis anasi sp. nov. from the mallard duck (Anas platyrhynchos)

Liuda Kutkien?; Petras Prakas; Aniolas Sruoga; Dalius Butkauskas

215

Description of Sarcocystis anasi sp. nov. and Sarcocystis albifronsi sp. nov. in birds of the order Anseriformes.  

PubMed

On the basis of the already published morphological, 18S rDNA, 28S rDNA data (Kutkien? et al., Parasitol Res 99:562-565, 2006; Parasitol Res 102:691-696, 2008; Parasitol Res 104:329-336, 2009), and ITS-1 region investigation results of sarcocysts presented in this paper, Sarcocystis albifronsi sp. nov. from the white-fronted goose (Anser albifrons) and Sarcocystis anasi sp. nov. from the mallard duck (Anas platyrhynchos) are described. PMID:21845412

Kutkien?, Liuda; Prakas, Petras; Sruoga, Aniolas; Butkauskas, Dalius

2012-02-01

216

Effects of cell growth and a mobile genetic element on propagation of the phages SP16 and SP-beta in Bacillus subtilis  

E-print Network

Two studies were performed on Bacillus subtilis phages SP16 and SP-beta to characterize the effects of growth and an integrative conjugative element, ICEBs1, respectively. I found that B. subtilis strains are significantly ...

England, Eleina M. (Eleina Helen Maria)

2014-01-01

217

Lipid-protein interactions of hydrophobic proteins SP-B and SP-C in lung surfactant assembly and dynamics.  

PubMed

Phospholipids have the major role in pulmonary surfacant concerning its biophysical function of reducing surface tension at the alveolar air-liquid interface to facilitate respiratory mechanics. However, the presence of some specific, highly hydrophobic polypeptides is essential to modulate the physical behavior of phospholipids and to promote rapid formation of stable surface films that are able to produce surface tensions in the range of 0 dynes/cm during cyclic compression. The present review summarizes the available data on the parameters governing lipid-protein interactions of the hydrophobic surfactant proteins SP-B and SP-C with the main surfactant phospholipids. Lipid-protein interactions in surfactant have been studied in vitro using preparations reconstituted with very different methodological procedures. Conclusions concerning the role of hydrophobic surfactant proteins on the assembly of lipid-protein surfactant structures in vivo have been revised in this respect. This review presents the knowledge available on the disposition of SP-B and SP-C in surfactant structures, the mode, extent, selectivity, and stoichiometry of their lipid-protein interactions, and the effect of the proteins on structure and dynamics of surfactant bilayers and monolayers. Some considerations are given to possible concerted actions, under physiological conditions, of both proteins SP-B and SP-C. PMID:11699574

Pérez-Gil, J

2001-01-01

218

DNA sequences identical to Pneumocystis carinii f. sp. carinii and Pneumocystis carinii f. sp. hominis in samples of air spora.  

PubMed Central

Samples of ambient air collected with three different types of spore traps in a rural location were examined for the presence of Pneumocystis carinii by screening for P. carinii-specific DNA sequences by DNA amplification. Eleven spore trap samples were analyzed by nested PCR, using oligonucleotide primers designed for the gene encoding the mitochondrial large subunit rRNA of P. carinii f. sp. carinii and P. carinii f. sp. hominis. The samples were collected over a 3-year period during the months of May to September, with a range of sampling times from 9 to 240 h. One air sample from an animal facility housing P. carinii-infected rats was also examined. P. carinii-specific amplification products were obtained from samples from each of the spore traps. The amplification products from eight air samples were cloned and sequenced. The majority of the recombinants from each of these samples had sequences identical to those of P. carinii f. sp. carinii and P. carinii f. sp. hominis, and a number of clones had single-base differences. These data suggest that sequences identical to those of P. carinii f. sp. carinii and P. carinii f. sp. hominis can be detected in samples of air collected in a rural location and that P. carinii may be a component of the air spora of rural Oxfordshire. PMID:8784583

Wakefield, A E

1996-01-01

219

On the Fourth Diadema Species (Diadema-sp) from Japan  

PubMed Central

Four long-spined sea urchin species in the genus Diadema are known to occur around the Japanese Archipelago. Three species (D. savignyi, D. setosum, and D. paucispinum) are widely distributed in the Indo-Pacific Ocean. The fourth species was detected by DNA analysis among samples originally collected as D. savignyi or D. setosum in Japan and the Marshall Islands and tentatively designated as Diadema -sp, remaining an undescribed species. We analyzed nucleotide sequences of the cytochrome oxidase I (COI) gene in the “D. savignyi-like” samples, and found all 17 individuals collected in the mainland of Japan (Sagami Bay and Kyushu) to be Diadema-sp, but all nine in the Ryukyu Archipelago (Okinawa and Ishigaki Islands) to be D. savignyi, with large nucleotide sequence difference between them (11.0%±1.7 SE). Diadema-sp and D. savignyi shared Y-shaped blue lines of iridophores along the interambulacrals, but individuals of Diadema-sp typically exhibited a conspicuous white streak at the fork of the Y-shaped blue iridophore lines, while this feature was absent in D. savignyi. Also, the central axis of the Y-shaped blue lines of iridophores was approximately twice as long as the V-component in D. savignyi whereas it was of similar length in Diadema-sp. Two parallel lines were observed to constitute the central axis of the Y-shaped blue lines in both species, but these were considerably narrower in Diadema-sp. Despite marked morphological and genetic differences, it appears that Diadema-sp has been mis-identified as D. savignyi for more than half a century. PMID:25054386

Chow, Seinen; Kajigaya, Yoshikazu; Kurogi, Hiroaki; Niwa, Kentaro; Shibuno, Takuro; Nanami, Atsushi; Kiyomoto, Setuo

2014-01-01

220

75 FR 42411 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Daily Contract; SP-15 Financial Day...  

Federal Register 2010, 2011, 2012, 2013, 2014

...COMMISSION Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Daily Contract; SP-15 Financial Day-Ahead LMP Off-Peak Daily Contract; SP-15...LMP-Peak Daily Contract; NP-15 Financial Day- Ahead LMP Peak Daily Contract and...

2010-07-21

221

75 FR 42380 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Contract and SP-15 Financial Day-Ahead...  

Federal Register 2010, 2011, 2012, 2013, 2014

...COMMISSION Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Contract and SP-15 Financial Day-Ahead LMP Off-Peak Contract Offered for...determination whether the SP-15 \\2\\ Financial Day-Ahead LMP Peak (``SPM'') contract...

2010-07-21

222

Altererythrobacter aestiaquae sp. nov., isolated from seawater.  

PubMed

A Gram-stain-negative, coccoid- or oval-shaped, gliding bacterial strain, designated HDW-31(T), belonging to the class Alphaproteobacteria, was isolated from seawater of the Yellow Sea, Korea, and was subjected to a taxonomic study using a polyphasic approach. Strain HDW-31(T) grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3?% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HDW-31(T) fell within the clade comprising the genus Altererythrobacter, clustering with the type strains of Altererythrobacter luteolus and Altererythrobacter gangjinensis, with which strain HDW-31(T) exhibited 97.0 and 96.0?% sequence similarity values, respectively. Sequence similarities to the type strains of the other recognized species of the genus Altererythrobacter were 93.5-96.0?%. The DNA G+C content was 57.9 mol% and mean DNA-DNA relatedness between strain HDW-31(T) and the type strain of A. luteolus was 5.3?%. Strain HDW-31(T) contained Q-10 as the predominant ubiquinone and summed feature 8 (C18?:?1?7c and/or C18?:?1?6c), summed feature 3 (C16?:?1?6c and/or C16?:?1?7c) and C16?:?0 as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, a sphingoglycolipid, two unidentified glycolipids and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain HDW-31(T) is distinguishable from recognized species of the genus Altererythrobacter. On the basis of the data presented, strain HDW-31(T) is considered to represent a novel species of the genus Altererythrobacter, for which the name Altererythrobacter aestiaquae sp. nov. is proposed. The type strain is HDW-31(T) (?=?KCTC 42006(T)?=?CECT 8527(T)). PMID:25201916

Jung, Yong-Taek; Park, Sooyeon; Lee, Jung-Sook; Yoon, Jung-Hoon

2014-12-01

223

Chitinophaga eiseniae sp. nov., isolated from vermicompost.  

PubMed

A Gram-negative, rod-shaped bacterial strain, YC6729(T), was isolated from vermicompost collected at Masan, Korea, and its taxonomic position was investigated by a polyphasic taxonomic approach. Strain YC6729(T) grew optimally at 30 °C and at pH 6.5-8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC6729(T) belongs to the genus Chitinophaga in the family Chitinophagaceae. It was related most closely to Chitinophaga terrae KP01(T) (96.4?% 16S rRNA gene sequence similarity), Chitinophaga ginsengisegetis Gsoil 040(T) (96.1?%), Chitinophaga arvensicola IAM 12650(T) (96.1?%) and Chitinophaga pinensis DSM 2588(T) (93.3?%). Strain YC6729(T) contained MK-7 as the major menaquinone and homospermidine as the major polyamine. The fatty acids of strain YC6729(T) were iso-C(15?:?0), C(16?:?1)?5c, iso-C(17?:?0) 3-OH, C(16?:?0), anteiso-C(18?:?0) and/or C(18?:?2)?6,9c, iso-C(15?:?0) 2-OH and/or C(16?:?1)?7c, C(14?:?0), iso-C(15?:?0) 3-OH, iso-C(15?:?1) G, C(18?:?1)?5c, iso-C(15?:?1) I and/or C(13?:?0) 3-OH, C(13?:?0) 2-OH, C(16?:?0) 3-OH and unknown fatty acid ECL 13.565. The polar lipid profile contained phosphatidylethanolamine, unknown aminolipids and unknown lipids. The total DNA G+C content of strain YC6729(T) was 48.9 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed that strain YC6729(T) represents a novel species of the genus Chitinophaga, for which the name Chitinophaga eiseniae sp. nov. is proposed. The type strain is YC6729(T) (?=?KACC 13774(T) ?=?DSM 22224(T)). PMID:21057055

Yasir, Muhammad; Chung, Eu Jin; Song, Geun Cheol; Bibi, Fehmida; Jeon, Che Ok; Chung, Young Ryun

2011-10-01

224

Enantiospecific sp2-sp3 coupling of secondary and tertiary boronic esters  

NASA Astrophysics Data System (ADS)

The cross-coupling of boronic acids and related derivatives with sp2 electrophiles (the Suzuki-Miyaura reaction) is one of the most powerful C-C bond formation reactions in synthesis, with applications that span pharmaceuticals, agrochemicals and high-tech materials. Despite the breadth of its utility, the scope of this Nobel prize-winning reaction is rather limited when applied to aliphatic boronic esters. Primary organoboron reagents work well, but secondary and tertiary boronic esters do not (apart from a few specific and isolated examples). Through an alternative strategy, which does not involve using transition metals, we have discovered that enantioenriched secondary and tertiary boronic esters can be coupled to electron-rich aromatics with essentially complete enantiospecificity. As the enantioenriched boronic esters are easily accessible, this reaction should find considerable application, particularly in the pharmaceutical industry where there is growing awareness of the importance of, and greater clinical success in, creating biomolecules with three-dimensional architectures.

Bonet, Amadeu; Odachowski, Marcin; Leonori, Daniele; Essafi, Stephanie; Aggarwal, Varinder K.

2014-07-01

225

Bradyrhizobium pachyrhizi sp. nov. and Bradyrhizobium jicamae sp. nov., isolated from effective nodules of Pachyrhizus erosus.  

PubMed

Several strains isolated from the legume Pachyrhizus erosus were characterized on the basis of diverse genetic, phenotypic and symbiotic approaches. These novel strains formed two groups closely related to Bradyrhizobium elkanii according to their 16S rRNA gene sequences. Strains PAC48T and PAC68T, designated as the type strains of these two groups, presented 99.8 and 99.1% similarity, respectively, in their 16S rRNA gene sequences with respect to B. elkanii USDA 76T. In spite of these high similarity values, the analysis of additional phylogenetic markers such as atpD and glnII genes and the 16S-23S intergenic spacer (ITS) showed that strains PAC48T and PAC68T represented two separate novel species of the genus Bradyrhizobium with B. elkanii as their closest relative. Phenotypic differences among the novel strains isolated from Pachyrhizus and B. elkanii were found regarding the assimilation of carbon sources and antibiotic resistance. All these differences were congruent with DNA-DNA hybridization analysis which revealed 21% genetic relatedness between strains PAC48T and PAC68T and 46% and 25%, respectively, between these strains and B. elkanii LMG 6134T. The nodD and nifH genes of strains PAC48T and PAC68T were phylogenetically divergent from those of bradyrhizobia species that nodulate soybean. Soybean was not nodulated by the novel Pachyrhizus isolates. Based on the genotypic and phenotypic data obtained in this study, the new strains represent two novel species for which the names Bradyrhizobium pachyrhizi sp. nov. (type strain PAC48T=LMG 24246T=CECT 7396T) and Bradyrhizobium jicamae sp. nov. (type strain PAC68T=LMG 24556T=CECT 7395T) are proposed. PMID:19567584

Ramírez-Bahena, Martha Helena; Peix, Alvaro; Rivas, Raúl; Camacho, María; Rodríguez-Navarro, Dulce N; Mateos, Pedro F; Martínez-Molina, Eustoquio; Willems, Anne; Velázquez, Encarna

2009-08-01

226

Methylophilus flavus sp. nov. and Methylophilus luteus sp. nov., aerobic, methylotrophic bacteria associated with plants.  

PubMed

Novel yellow, obligately methylotrophic and restricted facultatively methylotrophic bacteria, respectively designated strains Ship(T) and Mim(T), with the ribulose monophosphate pathway of C(1) assimilation are described. Cells were strictly aerobic, Gram-negative, asporogenous, non-motile rods that multiply by binary fission, were mesophilic and neutrophilic and synthesized indole-3-acetic acid and exopolysaccharide. The predominant cellular fatty acids were C(16?:?0) and C(16?:?1). The major ubiquinone was Q-8. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol; diphosphatidylglycerol was absent. The two strains lacked ?-ketoglutarate dehydrogenase and glutamate dehydrogenase. They assimilated ammonium via the glutamate cycle enzymes glutamine synthetase and glutamate synthase. The DNA G+C contents of strains Ship(T) and Mim(T) were 50.7 and 54.5 mol% (T(m)), respectively. The level of 16S rRNA gene sequence similarity between these strains was very high (99.8?%) but they shared a low level of DNA-DNA relatedness (44?%). Based on 16S rRNA gene sequence analysis and low levels of DNA-DNA relatedness with the type strains of recognized species of the genus Methylophilus (31-36?%), strains Ship(T) and Mim(T) are considered to represent novel species of the genus Methylophilus, for which the names Methylophilus flavus sp. nov. (type strain Ship(T) =DSM 23073(T) =VKM B-2547(T) =CCUG 58411(T)) and Methylophilus luteus sp. nov. (type strain Mim(T) =DSM 22949(T) =VKM B-2548(T) =CCUG 58412(T)) are proposed. PMID:20023062

Gogleva, Anna A; Kaparullina, Elena N; Doronina, Nina V; Trotsenko, Yuri A

2010-11-01

227

Martelella radicis sp. nov. and Martelella mangrovi sp. nov., isolated from mangrove sediment.  

PubMed

Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated BM5-7(T) and BM9-1(T) were isolated from soil of the root system of a mangrove forest. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates belong to the genus Martelella. The chemotaxonomic characteristics of these isolates included the presence of C19?:?0 cyclo ?8c and C18?:?1?7c as the major cellular fatty acids and Q-10 as the dominant ubiquinone. The genomic DNA G+C contents of strains BM5-7(T) and BM9-1(T) were 61.0 and 59.7 mol% (HPLC method), respectively. The 16S rRNA gene sequence similarity between the two strains was 98.1?%, but DNA-DNA hybridization indicated 44?% relatedness. Strains BM5-7(T) and BM9-1(T) exhibited 16S rRNA gene sequence similarities of 98.0-99.2?% and 97.7-98.1?%, respectively, with type strains of Martelella endophytica and Martelella mediterranea. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strains BM5-7(T) and BM9-1(T) are representatives of two novel species of the genus Martelella, for which the names Martelella radicis sp. nov. (type strain BM5-7(T)?=?DSM 28101(T)?=?LMG 27958(T)) and Martelella mangrovi sp. nov. (type strain BM9-1(T)?=?DSM 28102(T)?=?LMG 27959(T)) are proposed. PMID:24944336

Zhang, De-Chao; Margesin, Rosa

2014-09-01

228

Hymenobacter koreensis sp. nov. and Hymenobacter saemangeumensis sp. nov., isolated from estuarine water.  

PubMed

Two Gram-reaction-negative, rod-shaped, non-motile and red-pink-pigmented bacterial strains, designated GYR3077(T) and GSR0100(T), were isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do, South Korea, and were characterized using a polyphasic approach. 16S rRNA genes of strains GYR3077(T) and GSR0100(T) exhibited sequence similarities of 95.9?% to Hymenobacter deserti ZLB-3(T) and 96.6?% to Hymenobacter soli PB17(T), respectively, and indicated that these isolates belonged to the phylum Bacteroidetes. The major cellular fatty acids present in the two isolates were iso-C15?:?0, C16?:?1?5c, summed feature 4 (iso-C17?:?1 I and/or anteiso-C17?:?1 B) and summed feature 3 (C16?:?1?7c and/or C16?:?1?6c). The major respiratory quinone and polyamine patterns were menaquinone-7 and sym-homospermidine, characteristic of the genus Hymenobacter. Flexirubin-type pigments were absent in both strains. The DNA G+C contents of strains GYR3077(T) and GSR0100(T) were 60.2 mol% and 61.9 mol%, respectively. The major polar lipid of strains GYR3077(T) and GSR0100(T) was phosphatidylethanolamine. Based on the morphological and physiological properties, strains GYR3077(T) and GSR0100(T) were considered to represent two novel species of the genus Hymenobacter, for which the names Hymenobacter koreensis sp. nov. (type strain GYR3077(T)?=?KACC 16451(T)?=?JCM 17924(T)) and Hymenobacter saemangeumensis sp. nov. (type strain GSR0100(T)?=?KACC 16452(T)?=?JCM 17923(T)) are proposed. PMID:23907229

Kang, Ji Young; Chun, Jeesun; Choi, Ahyoung; Moon, Sung Hyun; Cho, Jang-Cheon; Jahng, Kwang Yeop

2013-12-01

229

Description of Leifsonia kafniensis sp. nov. and Leifsonia antarctica sp. nov.  

PubMed

Strains KFC-22(T) and SPC-20(T) are yellow-pigmented, Gram-positive, aerobic, non-motile, rod-shaped bacteria that were isolated from a soil sample near the Kafni glacier in the Himalayan mountain ranges in India, and from a spade core sediment sample from the Antarctic Ocean at Larsemann Hill, respectively. In both cases, the cell-wall peptidoglycan contained 2,4-diaminobutyric acid as the diamino acid, anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) were the predominant fatty acids and MK-11 was the major isoprenoid quinone in the cell membrane. On the basis of the above-mentioned characteristics, both strains can be assigned to the genus Leifsonia. The strains share 16S rRNA gene sequence similarity of 97.7 % and DNA relatedness of only 10 %, indicating that they represent different species. A blast analysis indicated that Leifsonia pindariensis PON10(T) was the closest phylogenetic neighbour of strains SPC-20(T) and KFC-22(T), showing 16S rRNA gene sequence similarities of 97.3 and 97.7 %, respectively. However, at the whole-genome level, strains KFC-22(T) and SPC-20(T) shared 42 and 11 % DNA-DNA relatedness, respectively, with L. pindariensis PON10(T). In addition, both strains exhibited several phenotypic differences with respect to L. pindariensis PON10(T). Thus, on the basis of the differences that the two strains exhibited with respect to L. pindariensis, both were identified as representing novel species of the genus Leifsonia, for which the names Leifsonia kafniensis sp. nov. (type strain KFC-22(T) =NCCB 100216(T) =LMG 24362(T)) and Leifsonia antarctica sp. nov. (type strain SPC-20(T) =NCCB 100227(T) =LMG 24541(T)) are proposed. PMID:19502314

Pindi, Pavan Kumar; Kishore, K Hara; Reddy, G S N; Shivaji, S

2009-06-01

230

Methylobacterium haplocladii sp. nov. and Methylobacterium brachythecii sp. nov., isolated from bryophytes.  

PubMed

Pink-pigmented, facultatively methylotrophic bacteria, strains 87e(T) and 99b(T), were isolated from the bryophytes Haplocladium microphyllum and Brachythecium plumosum, respectively. The cells of both strains were Gram-reaction-negative, motile, non-spore-forming rods. On the basis of 16S rRNA gene sequence similarity, strains 87e(T) and 99b(T) were found to be related to Methylobacterium organophilum ATCC 27886(T) (97.1% and 97.7%, respectively). Strains 87e(T) and 99b(T) showed highest 16S rRNA gene similarity to Methylobacterium gnaphalii 23e(T) (98.3 and 99.0%, respectively). The phylogenetic similarities to all other species of the genus Methylobacterium with validly published names were less than 97%. Major cellular fatty acids of both strains were C(18:1)?7c and C(18:0). The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrix-assisted, laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains 87e(T) and 99b(T) from their phylogenetically closest relatives. We propose that strains 87e(T) and 99b(T) represent novel species within the genus Methylobacterium, for which the names Methylobacterium haplocladii sp. nov. (type strain 87e(T)?=DSM 24195(T)?=NBRC 107714(T)) and Methylobacterium brachythecii sp. nov. (type strain 99b(T)?=DSM 24105(T)?=NBRC 107710(T)) are proposed. PMID:23475343

Tani, Akio; Sahin, Nurettin

2013-09-01

231

Hymenobacter latericoloratus sp. nov. and Hymenobacter luteus sp. nov., isolated from freshwater sediment.  

PubMed

Two novel Gram-stain negative, non-motile, rod-shaped and aerobic bacterial strains, designated YIM 77920(T) and YIM 77921(T), were isolated from freshwater sediment of Jiuxiang cave, a tourism cave located in Yiliang county, Yunnan province, south-west China. The 16S rRNA gene sequences of strains YIM 77920(T) and YIM 77921(T) exhibited sequence similarities of 96.59 and 96.66 % to Hymenobacter xinjiangensis X2-Y(T), respectively, and indicated that the two isolates belong to the genus Hymenobacter. The major fatty acids present in the two strains were identified as C16:1 ?5c, iso-C15:0 and Summed Feature 4 (C17:1 anteiso B/iso I). MK-7 was identified as the respiratory quinone component for both strains. The polar lipids profile of strain YIM 77920(T) was found to consist of phosphatidylethanolamine, four unidentified polar lipids, three unidentified aminophospholipids, two unidentified phospholipids and two unidentified aminolipids, while that of strain YIM 77921(T) consisted of phosphatidylethanolamine, four unidentified polar lipids, two unidentified aminolipids, one unidentified phospholipid and four unidentified aminophospholipids. The DNA G+C contents of strains YIM 77920(T) and YIM 77921(T) were determined to be 57.5 and 59.6 mol%, respectively. DNA-DNA hybridization between them had a low value (56.55 %). Based on the morphological and physiological properties, and phylogenetic analyses, strains YIM 77920(T) and YIM 77921(T) are considered to represent two novel species of the genus Hymenobacter, for which the names Hymenobacter latericoloratus sp. nov. (type strain YIM 77920(T) = JCM 30327(T) = CCTCC AB 2012949(T)) and Hymenobacter luteus sp. nov. (type strain YIM 77921(T) = JCM 30328(T) = CCTCC AB 2012947(T)) are proposed. PMID:25348876

Liu, Lan; Zhou, En-Min; Jiao, Jian-Yu; Manikprabhu, Deene; Ming, Hong; Liu, Wei-Hong; Hozzein, Wael N; Shu, Wen-Sheng; Li, Wen-Jun

2015-01-01

232

Vibrio variabilis sp. nov. and Vibrio maritimus sp. nov., isolated from Palythoa caribaeorum.  

PubMed

Two novel vibrio isolates (R-40492(T) and R-40493(T)) originating from the zoanthid Palythoa caribaeorum in Brazil in 2005 were taxonomically characterized by means of a polyphasic approach comprising multilocus sequence analysis (MLSA), DNA-DNA hybridization (DDH), ?T(m) analysis and phenotypic characterization. Phylogenetic analysis based on 16S rRNA gene sequences showed that R-40492(T) and R-40493(T) fell within the genus Vibrio and were most closely related to each other with 99% similarity; similarities of these two novel isolates towards Vibrio neptunius LMG 20536(T), Vibrio coralliilyticus LMG 20984(T), Vibrio nigripulchritudo LMG 3896(T), Vibrio sinaloensis LMG 25238(T) and Vibrio brasiliensis LMG 20546(T) varied between 97.1 and 98.5%. DDH experiments showed that the two isolates had less than 15% relatedness to the phylogenetically most closely related Vibrio species. R-40492(T) and R-40493(T) had 55-57% relatedness to each other. The ?T(m) between R-40492(T) and R-40493(T) was 6.12 °C. In addition, MLSA of concatenated sequences (16S rRNA, ftsZ, gyrB, recA, rpoA, topA, pyrH and mreB; 6035 bp in length) showed that the two novel isolates formed a separate branch with less than 92% concatenated gene sequence similarity towards known species of vibrios. Two novel species are proposed to accommodate these novel isolates, namely Vibrio variabilis sp. nov. (type strain, R-40492(T)=LMG 25438(T)=CAIM 1454(T)) and Vibrio maritimus sp. nov. (type strain, R-40493(T)=LMG 25439(T)=CAIM 1455(T)). PMID:21296931

Chimetto, Luciane A; Cleenwerck, Ilse; Moreira, Ana Paula B; Brocchi, Marcelo; Willems, Anne; De Vos, Paul; Thompson, Fabiano L

2011-12-01

233

Rhodococcus cerastii sp. nov. and Rhodococcus trifolii sp. nov., two novel species isolated from leaf surfaces.  

PubMed

Two Gram-positive, non-endospore-forming rods, strains C5(T) and T8(T), were isolated from the phyllospheres of Cerastium holosteoides and Trifolium repens, respectively, and were studied in detail for their taxonomic position. 16S rRNA gene sequence analysis allocated both isolates clearly to the genus Rhodococcus. Isolate C5(T) was most closely related to Rhodococcus fascians and Rhodococcus yunnanensis, showing 99.2?% gene sequence similarity to both species. Strain T8(T) revealed the highest 16S rRNA gene sequence similarity to Rhodococcus corynebacterioides (98.8?%) and Rhodococcus kroppenstedtii (98.6?%). The quinone system of both strains was composed of dihydrogenated menaquinones with eight (major amount) as well as nine, seven and six isoprenoid units (MK-8H2, MK-9H2 MK-7H2 MK-6H2).The polar lipid profiles of strains C5(T) and T8(T) consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. Additionally, strain C5(T) contained one unknown glycolipid, and strain T8(T) three unknown aminolipids. The fatty acid profiles contained major amounts of C16?:?0, C18?:?1?9c and 10-methyl C18?:?0, which supported the grouping of the two isolates in the genus Rhodococcus. Physiological/biochemical characterization and DNA-DNA hybridizations with the type strains of the most closely related species allowed a clear phenotypic and genotypic differentiation of both strains. For this reason, we propose strain C5(T) (?=?LMG 26203(T) ?=?CCM 7906(T)) as the type strain of a novel species with the name Rhodococcus cerastii sp. nov., and strain T8(T) (?=?LMG 26204(T) ?=?CCM 7905(T)) as the type strain of a second novel species with the name Rhodococcus trifolii sp. nov. PMID:22685110

Kämpfer, P; Wellner, S; Lohse, K; Lodders, N; Martin, K

2013-03-01

234

Pseudochelatococcus lubricantis gen. nov., sp. nov. and Pseudochelatococcus contaminans sp. nov. from coolant lubricants.  

PubMed

Two Gram-negative, rod-shaped, non-spore-forming bacteria, isolated from metal working fluids were investigated to determine their taxonomic positions. On the basis of 16S rRNA gene sequence phylogeny, both strains (MPA 1113(T) and MPA 1105(T)) formed a distinct cluster with 97.7?% sequence similarity between them, which was in the vicinity of members of the genera Methylobacterium, Camelimonas, Chelatococcus, Bosea, Salinarimonas and Microvirga to which they showed low sequence similarities (below 94?%). The predominant compounds in the polyamine pattern and in the quinone system of the two strains were spermidine and ubiquinone Q-10, respectively. The polar lipid profiles were composed of the major compounds: phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, major or moderate amounts of diphosphatidylglycerol, two unidentified glycolipids and three unidentified aminolipids. Several minor lipids were also detected. The major fatty acids were either C19?:?0 cyclo ?8c or C18?:?1?7c. The results of fatty acid analysis and physiological and biochemical tests allowed both, the genotypic and phenotypic differentiation of the isolates from each other, while the chemotaxonomic traits allowed them to be differentiated from the most closely related genera. In summary, low 16S rRNA gene sequence similarities and marked differences in polar lipid profiles, as well as in polyamine patterns, is suggestive of a novel genus for which the name Pseudochelatococcus gen. nov. is proposed. MPA 1113(T) (?=?CCM 8528(T)?=?LMG 28286(T)?=?CIP 110802(T)) and MPA 1105(T) (?=?CCM 8527(T)?=?LMG 28285(T)) are proposed to be the type strains representing two novel species within the novel genus, Pseudochelatococcus gen. nov., for which the names Pseudochelatococcus lubricantis sp. nov. and Pseudochelatococcus contaminans sp. nov. are suggested, respectively. PMID:25301542

Kämpfer, Peter; Glaeser, Stefanie P; Gräber, Marco; Rabenstein, Andreas; Kuever, Jan; Busse, Hans-Jürgen

2015-01-01

235

From SO/Sp instantons to W-algebra blocks  

NASA Astrophysics Data System (ADS)

We study instanton partition functions for mathcal{N} = 2 superconformal Sp(1) and SO(4) gauge theories. We find that they agree with the corresponding U(2) instanton partitions functions only after a non-trivial mapping of the microscopic gauge couplings, since the instanton counting involves different renormalization schemes. Geometrically, this mapping relates the Gaiotto curves of the different realizations as double coverings. We then formulate an AGT-type correspondence between Sp(1)/SO(4) instanton partition functions and chiral blocks with an underlying mathcal{W}left( {2,2} right) -algebra symmetry. This form of the correspondence eliminates the need to divide out extra U(1) factors. Finally, to check this correspondence for linear quivers, we compute expressions for the Sp(1) × SO(4) half-bifundamental.

Hollands, Lotte; Keller, Christoph A.; Song, Jaewon

2011-03-01

236

Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09*  

PubMed Central

Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity. PMID:25367790

Chen, Yun-mei; Xu, Hai-yang; Wang, Yang; Zhang, Jian-fa; Wang, Shi-ming

2014-01-01

237

Comparison of green algae Cladophora sp. and Enteromorpha sp. as potential biomonitors of chemical elements in the southern Baltic.  

PubMed

The contents of Cd, Cu, Ni, Pb, Zn, Mn, K, Na, Ca and Mg were determined in the green algae Cladophora sp. from coastal and lagoonal waters of the southern Baltic. Factor analysis demonstrated spatial differences between concentration of chemical elements. The algae from the southern Baltic contained more Na and K while the anthropogenic impact of Cu, Pb and Zn was observed in the case of Cladophora sp. and Enteromorpha sp. from the Gulf of Gdansk at the vicinity of Gdynia. This area is exposed to emission of heavy metals from municipal and industrial sources with the main contribution of shipbuilding industry and seaport. The statistical evaluation of data has demonstrated that there exists a correlation between concentrations of Cu, Pb and Zn in both green algae collected at the same time and sampling sites of the Gulf of Gdansk. Our results show that in the case of absence of one species in the investigated area it is still possible to continue successfully the biomonitoring studies with its replacing by second one, i.e. Cladophora sp. by Enteromorpha sp. and vice versa; in consequence reliable results may be obtained. PMID:17719620

Zbikowski, Rados?aw; Szefer, Piotr; Lata?a, Adam

2007-11-15

238

Interrelationships between Bacillus sp. CHEP5 and Bradyrhizobium sp. SEMIA6144 in the induced systemic resistance against Sclerotium rolfsii and symbiosis on peanut plants.  

PubMed

Plant-growth-promoting bacteria are often used to enhance crop yield and for biological control of phytopathogens. Bacillus sp. CHEP5 is a biocontrol agent that induces systemic resistance (ISR) in Arachis hypogaea L. (peanut) against Sclerotium rolfsii, the causal agent of root and stem wilt. In this work, the effect of the co-inoculation of Bacillus sp. CHEP5 and the peanut nodulating strain Bradyrhizobium sp. SEMIA 6144 was studied on induction of both systemic resistance and nodulation processes. Bradyrhizobium sp. SEMIA 6144 did not affect the ability of Bacillus sp. CHEP5 to protect peanut plants from S. rolfsii by ISR and the priming in challenged-plants, as evidenced by an increment in phenylalanine ammonia-lyase enzyme activity. Additionally, the capacity of Bradyrhizobium sp. SEMIA 6144 to induce nodule formation in pathogen-challenged plants was improved by the presence of Bacillus sp. CHEP5. PMID:25431416

Figueredo, Maria Soledad; Tonelli, Maria Laura; Taurian, Tania; Angelini, Jorge; Ibanez, Fernando; Valetti, Lucio; Munoz, Vanina; Anzuay, Maria Soledad; Luduena, Liliana; Fabra, Adriana

2014-12-01

239

Fuels irradiation testing for the SP-100 program  

NASA Technical Reports Server (NTRS)

An SP-100 fuel pin irradiation testing program is well on the way to providing data for performance correlations and demonstrating the lifetime and safety of the fuel system of the compact lithium-cooled reactor. Key SP-100 fuel performance issues addressed are the need for low fuel swelling and low fission gas release to minimize cladding strain, and the need for barrier integrity to prevent fuel/cladding chemical interaction. This paper provides a description of the irradiation test program that addresses these key issues and summarizes recent results of posttest examinations including data obtained at 6 atom percent goal burnup.

Makenas, Bruce J.; Hales, Janell W.; Ward, Alva L.

1991-01-01

240

SpF: Enabling Petascale Performance for Pseudospectral Dynamo Models  

NASA Astrophysics Data System (ADS)

Pseudospectral (PS) methods possess a number of characteristics (e.g., efficiency, accuracy, natural boundary conditions) that are extremely desirable for dynamo models. Unfortunately, dynamo models based upon PS methods face a number of daunting challenges, which include exposing additional parallelism, leveraging hardware accelerators, exploiting hybrid parallelism, and improving the scalability of global memory transposes. Although these issues are a concern for most models, solutions for PS methods tend to require far more pervasive changes to underlying data and control structures. Further, improvements in performance in one model are difficult to transfer to other models, resulting in significant duplication of effort across the research community. We have developed an extensible software framework for pseudospectral methods called SpF that is intended to enable extreme scalability and optimal performance. High-level abstractions provided by SpF unburden applications of the responsibility of managing domain decomposition and load balance while reducing the changes in code required to adapt to new computing architectures. The key design concept in SpF is that each phase of the numerical calculation is partitioned into disjoint numerical 'kernels' that can be performed entirely in-processor. The granularity of domain-decomposition provided by SpF is only constrained by the data-locality requirements of these kernels. SpF builds on top of optimized vendor libraries for common numerical operations such as transforms, matrix solvers, etc., but can also be configured to use open source alternatives for portability. SpF includes several alternative schemes for global data redistribution and is expected to serve as an ideal testbed for further research into optimal approaches for different network architectures. In this presentation, we will describe the basic architecture of SpF as well as preliminary performance data and experience with adapting legacy dynamo codes. We will conclude with a discussion of planned extensions to SpF that will provide pseudospectral applications with additional flexibility with regard to time integration, linear solvers, and discretization in the radial direction.

Jiang, W.; Clune, T.; Vriesema, J.; Gutmann, G.

2013-12-01

241

Restricted Schurs and correlators for SO( N ) and Sp( N )  

NASA Astrophysics Data System (ADS)

In a recent work, restricted Schur polynomials have been argued to form a complete orthogonal set of gauge invariant operators for the 1/4-BPS sector of free = 4 super Yang-Mills theory with an SO( N ) gauge group. In this work, we extend these results to the theory with an Sp( N ) gauge group. Using these operators, we develop techniques to compute correlation functions of any multi-trace operators with two scalar fields exactly in the free theory limit for both SO( N ) and Sp( N ).

Kemp, Garreth

2014-08-01

242

Cotoneaster sp. poisoning in a llama (Lama glama).  

PubMed

A case of poisoning with a Cotoneaster sp. is described in a llama (Lama glama). The clinical signs were dyspnea, with congested mucous membranes, chewing, recumbency, and opisthotonos. Several hours after ingestion of the plant, the llama died, despite treatment with sodium thiosulfate and nitrite. Necropsy revealed approximately 676 g of leaves and fruit of Cotoneaster in the first compartment of the stomach. The blood was light red and did not clot. Because Cotoneaster sp. contains low concentrations of cyanogenic glycosides, toxicosis occurs only following massive ingestion of plant material. PMID:19286507

Grüss, Aurélie; Priymenko, Nathalie

2009-03-01

243

A comparative study on phyllosphere nitrogen fixation by newly isolated Corynebacterium sp. & Flavobacterium sp. and their potentialities as biofertilizer.  

PubMed

A number of nitrogen fixing bacteria has been isolated from forest phyllosphere on the basis of nitrogenase activity. Among them two best isolates are selected and identified as Corynebacterium sp. AN1 & Flavobacterium sp. TK2 able to reduce 88 and 132 n mol of acetylene (10(8)cells(-1)h(-1)) respectively. They were grown in large amount and sprayed on the phyllosphere of maize plants as a substitute for nitrogenous fertilizer. Marked improvements in growth and total nitrogen content of the plant have been observed by the application of these nitrogen-fixing bacteria. An average 30-37% increase in yield was obtained, which is nearer to chemical fertilizer treatment. Comparatively better effect was obtained by application of Flavobacterium sp. PMID:15362287

Giri, S; Pati, B R

2004-01-01

244

Fusarium euwallaceae sp. nov.--a symbiotic fungus of Euwallacea sp., an invasive ambrosia beetle in Israel and California.  

PubMed

The invasive Asian ambrosia beetle Euwallacea sp. (Coleoptera, Scolytinae, Xyleborini) and a novel Fusarium sp. that it farms in its galleries as a source of nutrition causes serious damage to more than 20 species of live trees and pose a serious threat to avocado production (Persea americana) in Israel and California. Adult female beetles are equipped with mandibular mycangia in which its fungal symbiont is transported within and from the natal galleries. Damage caused to the xylem is associated with disease symptoms that include sugar or gum exudates, dieback, wilt and ultimately host tree mortality. In 2012 the beetle was recorded on more than 200 and 20 different urban landscape species in southern California and Israel respectively. Euwallacea sp. and its symbiont are closely related to the tea shot-hole borer (E. fornicatus) and its obligate symbiont, F. ambrosium occurring in Sri Lanka and India. To distinguish these beetles, hereafter the unnamed xyleborine in Israel and California will be referred to as Euwallacea sp. IS/CA. Both fusaria exhibit distinctive ecologies and produce clavate macroconidia, which we think might represent an adaption to the species-specific beetle partner. Both fusaria comprise a genealogically exclusive lineage within Clade 3 of the Fusarium solani species complex (FSSC) that can be differentiated with arbitrarily primed PCR. Currently these fusaria can be distinguished only phenotypically by the abundant production of blue to brownish macroconidia in the symbiont of Euwallacea sp. IS/CA and their rarity or absence in F. ambrosium. We speculate that obligate symbiosis of Euwallacea and Fusarium, might have driven ecological speciation in these mutualists. Thus, the purpose of this paper is to describe and illustrate the novel, economically destructive avocado pathogen as Fusarium euwallaceae sp. nov. S. Freeman et al. PMID:23928415

Freeman, S; Sharon, M; Maymon, M; Mendel, Z; Protasov, A; Aoki, T; Eskalen, A; O'Donnell, K

2013-01-01

245

Natural infection by Giardia sp. and Cryptosporidium sp. in dairy goats, associated with possible risk factors of the studied properties.  

PubMed

Visits were made to six farms raising dairy goats located in the mountain region of the state of Rio de Janeiro, Brazil, seeking to identify parasitism by Giardia sp. and Cryptosporidium sp. During the visits, fecal samples were collected from approximately 10% of the stock of each property. A questionnaire was given to the keepers on each property to obtain data for epidemiological analysis. A total of 105 fecal samples was collected, 56 from adult animals (over 12 months of age) and 49 from juvenile animals (less than 12 months). The fecal material was processed and subjected to the centrifuge-flotation technique and to staining with safranine-methylene blue. Protozoans of the genus Cryptosporidium were found at two properties, where the hygiene conditions of the installations were considered average and the stalls were made of wood slats raised from the ground. A total of five (4.8%) of the samples was found to be positive for the presence of this protozoan, all from juvenile animals. Cysts of the genus Giardia were found at two properties. Of the 105 samples analyzed, the protozoan was detected in 15 (14.3%), all in juvenile animals, and animals in the age range of 1-3 months had significantly more infections. Age, sanitary condition of the stalls and stalls made of wood slats and raised from the ground, can be pointed out as possible risk factors for infection by Giardia sp. and Cryptosporidium sp. The study reports for the first time the occurrence of Cryptosporidium sp. and Giardia sp. in goats in the state of Rio de Janeiro. PMID:16105719

Bomfim, T C B; Huber, F; Gomes, R S; Alves, L L

2005-11-25

246

Nocardioides ultimimeridianus sp. nov. and Nocardioides maradonensis sp. nov., isolated from rhizosphere soil.  

PubMed

Two novel Gram-reaction-positive, rod-shaped actinobacterial strains, designated RP-B26(T) and RP-B30(T), were isolated from rhizosphere soil of a cliff-associated plant (Peucedanum japonicum Thunb.) which was collected from Mara Island, Republic of Korea. The colonies of the isolates were circular, smooth, convex and moderately yellow-light-yellow in colour. 16S rRNA gene sequence analysis revealed that the isolates belonged to the family Nocardioidaceae and formed two distinct sublineages within the radiation of the genus Nocardioides. 16S rRNA gene sequence similarity between the isolates was 98.2 %. The closest phylogenetic neighbours of strain RP-B26(T) were Nocardioides humi DCY24(T) and Nocardioides kongjuensis A2-4(T) with 97.4 and 97.0 % 16S rRNA gene sequence similarity, respectively, whereas 16S rRNA gene sequence similarities between strain RP-B30(T) and N. humi DCY24(T) and N. kongjuensis A2-4(T) were 96.5 and 96.0 %, respectively. Both of the isolates contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell walls. The predominant menaquinone was MK-8(H(4)). The polar lipids were phosphatidylinositol and phosphatidylglycerol. The fatty acid profiles of the isolates were characterized by the presence of saturated, unsaturated, 10-methyl and hydroxyl fatty acids, with small amounts of branched fatty acids. The DNA G+C contents of strains RP-B26(T) and RP-B30(T) were 73.0 and 71.7 mol%, respectively. Levels of DNA-DNA relatedness between the isolates were 44.9 ± 1.5 % (thermal renaturation method) and 43.2 % (photobiotin-labelled method); the isolates showed low DNA-DNA relatedness values (<11 %) to the most closely related strain, N. humi KCTC 19265(T). On the basis of the phenotypic, genotypic and DNA-DNA hybridization data presented here, the isolates are considered to represent two novel species of the genus Nocardioides, for which the names Nocardioides ultimimeridianus sp. nov. (type strain RP-B26(T) ?=?KCTC 19368(T) ?= DSM 19768(T)) and Nocardioides maradonensis sp. nov. (type strain RP-B30(T) ?=?KCTC 19384(T) ?= DSM 19769(T)) are proposed. PMID:20833879

Lee, Dong Wan; Lee, Soo-Young; Yoon, Jung-Hoon; Lee, Soon Dong

2011-08-01

247

Methylosarcina fibrata gen. nov., sp. nov. and Methylosarcina quisquiliarum sp.nov., novel type 1 methanotrophs.  

PubMed

Two novel species of obligate methane-oxidizing bacteria, isolated from landfill soil, were characterized. Both strains were unusual in that some members of the population grew in irregularly shaped, refractile cell packets that resembled sarcina-like clusters. Electron microscopy revealed that the cell packets were covered with a slime layer and the cells contained many large granular inclusion bodies. The individual cells of each strain were sometimes motile and had differing morphologies. Isolate AML-C10T was always coccoidal in shape, and the cells were covered with extracellular fibrils. Isolate AML-D4T was pleomorphic, changing from rod to coccal form, sometimes exhibiting an unusual fusiform morphology. AML-D4T lacked the extensive fibrillar matrix observed with AML-C10T. Both strains utilized only methane and methanol as carbon sources. In stationary phase, the cells of each strain swelled in size and formed cysts. Aside from morphological differences, strains could also be distinguished from each other by cellular protein patterns, as well as by temperature and pH tolerances. 16S rDNA phylogenetic analysis showed that these are type I methanotrophs (family: Methylococcaceae) most closely related to the Methylobacter/Methylomicrobium clade, although they form a monophyletic grouping supported by moderately high bootstrap values. By 16S rDNA database searches, the most similar species to both isolates were Methylobacter spp. However, partial particulate methane monooxygenase sequence analysis suggested that these bacteria might be more closely related to Methylomicrobium than Methylobacter. Furthermore, cellular fatty acid profiles of the strains more closely resemble those of Methylomicrobium, although the absence of significant levels of 16:1omega5c argues for the uniqueness of these two strains. On the basis of the results described here, it is proposed that a new genus should be created, Methylosarcina gen. nov., harbouring two species, Methylosarcina fibrata sp. nov. (type species) and Methylosarcina quisquiliarum sp. nov. The type strains are AML-C10T (= ATCC 700909T = DSM 13736T) and AML-D4T (= ATCC 700908T = DSM 13737T), respectively. PMID:11321107

Wise, M G; McArthur, J V; Shimkets, L J

2001-03-01

248

Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., Isolated from Sea Water  

PubMed Central

Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1T and A3d10T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4°C and 40°C, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C16:0, C16:1?7c, C18:1?9c and C18:1?7c. The G+C content of the DNA for strains R9SW1T and A3d10T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved protein-coding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1T clusters with M. algicola DG893T sharing 99.40%, and A3d10T clusters with M. sediminum R65T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1T and A3d10T represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1T (?=? LMG 27497T ?=? JCM 19399T ?=? CIP 110588T ?=? KMM 7502T) and Marinobacter similis sp. nov., with the type strain A3d10T (?=? JCM 19398T ?=? CIP 110589T ?=? KMM 7501T), are proposed. PMID:25198502

Ng, Hooi Jun; López-Pérez, Mario; Webb, Hayden K.; Gomez, Daniela; Sawabe, Tomoo; Ryan, Jason; Vyssotski, Mikhail; Bizet, Chantal; Malherbe, François; Mikhailov, Valery V.; Crawford, Russell J.; Ivanova, Elena P.

2014-01-01

249

Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov., isolated from a composting reactor.  

PubMed

Two strains, 8-4-E12(T) and 8-4-E13(T), were isolated from a biowaste composting reactor. Based on 16S rRNA gene sequences, both strains belong to the genus Bacillus. Strain 8-4-E12(T) was most closely related to the type strains of Bacillus shackletonii, B. acidicola, B. sporothermodurans and B. oleronius (96.4, 96.3, 96.0 and 95.6?% 16S rRNA gene similarity, respectively), whereas strain 8-4-E13(T) was most closely related to the type strain of Bacillus humi (96.5?% sequence similarity). Strains 8-4-E12(T) and 8-4-E13(T) shared 94?% 16S rRNA gene sequence similarity. The fatty acid profile of strain 8-4-E12(T) was dominated by saturated iso- and anteiso-branched fatty acids (iso-C15?:?0, anteiso-C15?:?0, anteiso-C17?:?0 and iso-C16?:?0), and also contained considerable amounts of C16?:?0. The fatty acid profile of strain 8-4-E13(T) showed a predominance of iso-C15?:?0 (65?%), with smaller amounts of other saturated branched-chain fatty acids along with an unsaturated alcohol. Both strains contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major polar lipids. Additionally, strain 8-4-E12(T) contained an unknown lipid and strain 8-4-E13(T) two unknown (amino-)phospholipids. The diagnostic diamino acid found in the cell-wall peptidoglycan of 8-4-E12(T) and 8-4-E13(T) was meso-diaminopimelic acid. The predominant menaquinone was MK-7. The results of physiological and biochemical tests also allowed phenotypic differentiation of the two strains from each other and from related Bacillus species. On the basis of their phylogenetic, phenotypic and chemotaxonomic properties, strains 8-4-E12(T) and 8-4-E13(T) represent novel species of the genus Bacillus, for which the names Bacillus pervagus sp. nov. (type strain 8-4-E12(T)?=?DSM 23947(T)?=?LMG 27601(T)) and Bacillus andreesenii sp. nov. (type strain 8-4-E13(T)?=?DSM 23948(T)?=?LMG 27602(T)) are proposed. PMID:24021730

Kosowski, Kornelia; Schmidt, Marie; Pukall, Rüdiger; Hause, Gerd; Kämpfer, Peter; Lechner, Ute

2014-01-01

250

Microbacterium kyungheense sp. nov. and Microbacterium jejuense sp. nov., isolated from salty soil.  

PubMed

Two novel strains, THG-C26(T) and THG-C31(T), were characterized using a polyphasic approach to determine their taxonomic positions. These two isolates were aerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped. 16S rRNA gene sequences and phenotypic features including chemotaxonomic characteristics indicated that the two isolates clearly represented members of the genus Microbacterium. The quinone systems of strains THG-C26(T) and THG-C31(T) contained MK-12/MK-13 as major menaquinones. The diamino acid in cell-wall hydrolysates of the two strains was ornithine. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polyamine pattern had spermidine as the predominant component. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipids. Phenotypic characteristics supported the affiliation of strains THG-C26(T) and THG-C31(T) to the genus Microbacterium. Chemotaxonomic data and DNA-DNA relatedness values allowed differentiation of these strains from other species of the genus Microbacterium with validly published names. Strains THG-C26(T) and THG-C31(T) showed highest 16S rRNA gene sequence similarities with Microbacterium resistens DMMZ 1710(T) (98.5%) and Microbacterium trichothecenolyticum IFO 15077(T) (98.8%), respectively, and the 16S rRNA gene sequence similarity between them was 99.0%. DNA-DNA hybridization values between the novel isolates and strains of other species of the genus Microbacterium with validly published names were 4-25%. Therefore, strains THG-C26(T) and THG-C31(T) are considered to represent two novel species of the genus Microbacterium, for which the names Microbacterium kyungheense sp. nov. [type strain THG-C26(T) (?= KACC 17124(T)?= JCM 18735(T))] and Microbacterium jejuense sp. nov. [type strain THG-C31(T) (?= KACC 17123(T)?= JCM 18734(T))] are proposed. PMID:24729394

Kook, MooChang; Son, Heung-Min; Yi, Tae-Hoo

2014-07-01

251

Structural studies of the polysaccharides from the lipopolysaccharides of Azospirillum brasilense Sp246 and SpBr14.  

PubMed

Lipopolysaccharides from closely related Azospirillum brasilense strains, Sp246 and SpBr14, were obtained by phenol-water extraction. Mild acid hydrolysis of the lipopolysaccharides followed by GPC on Sephadex G-50 resulted in polysaccharide mixtures. On the basis of sugar and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy data, it was concluded that both bacteria possess the same two distinct polysaccharides having structures 1 and 2: [structure: see text]. Structure 1 has been reported earlier for a polysaccharide of A. brasilense 54 [Fedonenko et al., 2011] whereas to our knowledge structure 2 has not been hitherto found in bacterial polysaccharides. PMID:25240180

Sigida, Elena N; Fedonenko, Yuliya P; Shashkov, Alexander S; Grinev, Vyacheslav S; Zdorovenko, Evelina L; Konnova, Svetlana A; Ignatov, Vladimir V; Knirel, Yuriy A

2014-10-29

252

Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov.  

PubMed

Strains of fluorescent pseudomonads, isolated from the phyllosphere of grasses, were analysed by a polyphasic approach in order to clarify their interspecific position. Classification on the basis of ribotyping revealed six genotypes; four of these, which could be differentiated clearly from each other and from Pseudomonas species with validly published names on the basis of phenotypic features, were chosen for detailed phylogenetic analysis. DNA-DNA hybridization studies among representative strains of the four genotypes and closely related Pseudomonas species, determined by comparison of 16S rDNA sequences, showed that three of the studied ribotypes represented novel species. Two of them were related to mainly saprophytic fluorescent pseudomonads and could be easily distinguished by a negative arginine dihydrolase reaction. One ribotype, also characterized by a negative arginine dihydrolase reaction, was closely related to potentially plant-pathogenic fluorescent pseudomonads and differed in certain phenotypic features from its phylogenetic neighbours. As a consequence of the phenotypic and phylogenetic analyses, Pseudomonas trivialis sp. nov. (type strain: P 513/19(T)=DSM 14937(T)=LMG 21464(T)), Pseudomonas poae sp. nov. (type strain: P 527/13(T)=DSM 14936(T)=LMG 21465(T)) and Pseudomonas congelans sp. nov. (type strain: P 538/23(T)=DSM 14939(T)=LMG 21466(T)) are proposed. PMID:13130034

Behrendt, Undine; Ulrich, Andreas; Schumann, Peter

2003-09-01

253

Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes.  

PubMed

Gram-negative, rod-shaped bacteria were isolated from root nodules of Lupinus polyphyllus, Lathyrus latifolius and Robinia pseudoacacia. Based on the 16S rRNA gene phylogeny, they were closely related to Bosea species (100-97?% similarity), belonging to the class Alphaproteobacteria, family Bradyrhizobiaceae. The closest relatives of LMG 26383(T), LMG 26379(T) and LMG 26381(T) were respectively the type strains of Bosea thiooxidans (99.6?%), B. eneae (98.3?%) and B. minatitlanensis (99.0?%). Chemotaxonomic data, including major fatty acid profiles, supported the assignment of our strains to the genus Bosea. Analysis of the concatenated sequences of five housekeeping genes (atpD, dnaK, gyrB, recA and rpoB) and the results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from each other and from the five Bosea species with validly published names. No nodA or nodC genes could be amplified, while nifH PCR gave non-specific products. On the basis of genotypic and phenotypic data, three novel species, Bosea lupini sp. nov. (type strain LMG 26383(T) ?=?CCUG 61248(T) ?=?R-45681(T)), Bosea lathyri sp. nov. (type strain LMG 26379(T) ?=?CCUG 61247(T) ?=?R-46060(T)) and Bosea robiniae sp. nov. (type strain LMG 26381(T) ?=?CCUG 61249(T) ?=?R-46070(T)), are proposed. PMID:22155761

De Meyer, Sofie E; Willems, Anne

2012-10-01

254

Phytohabitans flavus sp. nov., Phytohabitans rumicis sp. nov. and Phytohabitans houttuyneae sp. nov., isolated from plant roots, and emended description of the genus Phytohabitans.  

PubMed

An actinomycete strain, designated K09-0627(T), was isolated from the roots of an orchid collected in Okinawa Prefecture, Japan. Two actinomycete strains K11-0047(T) and K11-0057(T) were isolated from the roots of Rumex acetosa and Houttuynia cordata collected in Kanagawa Prefecture, Japan. 16S rRNA gene sequence analyses indicated that the isolates belonged to the genus Phytohabitans, and that they were closely related to each other and to Phytohabitans suffuscus K07-0523(T). The DNA-DNA relatedness values between the three isolates and Phytohabitans suffuscus were below 70%. On the basis of phylogenetic analysis, DNA-DNA relatedness values and phenotypic characteristics, the strains should be classified as novel species in the genus Phytohabitans, for which the names Phytohabitans flavus sp. nov. (type strain, K09-0627(T)=JCM 17387(T)=NBRC 107702(T)=DSM 45551(T)), Phytohabitans rumicis sp. nov. (type strain, K11-0047(T)=JCM 17829(T)=NBRC 108638(T)=BCC 48146(T)) and Phytohabitans houttuyneae sp. nov. (type strain, K11-0057(T)=JCM 17830(T)=NBRC 108639(T)=BCC 48147(T)) are proposed. PMID:22228668

Inahashi, Yuki; Matsumoto, Atsuko; Omura, Satoshi; Takahashi, Yoko

2012-11-01

255

Rhizobium calliandrae sp. nov., Rhizobium mayense sp. nov. and Rhizobium jaguaris sp. nov., rhizobial species nodulating the medicinal legume Calliandra grandiflora.  

PubMed

Calliandra grandiflora has been used as a medicinal plant for thousands of years in Mexico. Rhizobial strains were obtained from root nodules of C. grandiflora collected from different geographical regions in Chiapas and characterized by BOX-PCR, amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequence analysis. Most isolates corresponded to members of the genus Rhizobium and those not related to species with validly published names were further characterized by recA, atpD, rpoB and nifH gene phylogenies, phenotypic and DNA-DNA hybridization analyses. Three novel related species of the genus Rhizobium within the 'Rhizobium tropici group' share the same symbiovar that may be named sv. calliandrae. The names proposed for the three novel species are Rhizobium calliandrae sp. nov. (type strain, CCGE524(T)?=ATCC BAA-2435(T)?=CIP 110456(T)?=LBP2-1(T)), Rhizobium mayense sp. nov. (type strain, CCGE526(T)?=ATCC BAA-2446(T)?=?CIP 110454(T)?=NSJP1-1(T)) and Rhizobium jaguaris sp. nov. (type strain, CCGE525(T)?=ATCC BAA-2445(T)?=CIP 110453(T)?=SJP1-2(T)). PMID:23584283

Rincón-Rosales, Reiner; Villalobos-Escobedo, José M; Rogel, Marco A; Martinez, Julio; Ormeño-Orrillo, Ernesto; Martínez-Romero, Esperanza

2013-09-01

256

Increased Constituent Ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. and a Decrease in Microflora Diversity May Be Indicators of Ventilator-Associated Pneumonia: A Prospective Study in the Respiratory Tracts of Neonates  

PubMed Central

Ventilator-associated pneumonia (VAP) is a common complication and cause of death in neonates on mechanical ventilation. However, it is difficult to define the causes of VAP. To understand the causes of VAP, we undertook a prospective study based on the diversity of the microflora in VAP. The experimental group consisted of newborns who suffered from respiratory distress syndrome (RDS) and VAP, while the control group suffered from RDS without VAP. Sputa were collected within 1, 3, and 5 days of ventilation and were divided into six groups. DNA was extracted from the samples, and the 16S rDNA was PCR amplified, separated using denaturing gradient gel electrophoresis (DGGE), cloned and sequenced. The resulting sequences were compared using BLAST. The DGGE pictures were measured, and the richness, Shannon-Wiener index, and cluster maps were analyzed. No differences were found regarding the constituent ratio of any genus between the Non-VAP and VAP group within 1 day after intubation. After 1 to 3 days, the constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the VAP group were higher than those in the Non-VAP group, and the ratios of Serratia sp. and Achromobacter sp. were lower. After 3 to 5 days, the ratios of Klebsiella sp., Acinetobacter sp., Serratia sp., and Achromobacter sp. were lower than those in the Non-VAP group. The richness and Shannon-Wiener index of the Non-VAP group were higher than those of the VAP group from 1 to 3 days after intubation, while no differences were found within 1 day and from 3 to 5 days. We conclude that during the first three days of intubation, the microflora diversity in the lower respiratory tract was reduced due to VAP, and the greater constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the sputum may be indicators of VAP. PMID:24586277

Lu, Wei; Yu, Jialin; Ai, Qing; Liu, Dong; Song, Chao; Li, Luquan

2014-01-01

257

76 FR 73483 - Airworthiness Directives; Gulfstream Aerospace Corporation Model GV and GV-SP Airplanes  

Federal Register 2010, 2011, 2012, 2013, 2014

...Airworthiness Directives; Gulfstream Aerospace Corporation Model GV and GV-SP Airplanes...directive (AD) for certain Gulfstream Aerospace Corporation Model GV and GV-SP airplanes...identified in this AD, contact Gulfstream Aerospace Corporation, Technical...

2011-11-29

258

Forecasting the S&P 500 index using time series analysis and simulation methods  

E-print Network

The S&P 500 represents a diverse pool of securities in addition to Large Caps. A range of audiences are interested in the S&P 500 forecasts including investors, speculators, economists, government and researchers. The ...

Chan, Eric Glenn

2009-01-01

259

An Insight into the Diverse Roles of Surfactant Proteins, SP-A and SP-D in Innate and Adaptive Immunity  

PubMed Central

Surfactant proteins SP-A and SP-D are hydrophilic, collagen-containing calcium-dependent lectins, which appear to have a range of innate immune functions at pulmonary as well as extrapulmonary sites. These proteins bind to target ligands on pathogens, allergens, and apoptotic cells, via C-terminal homotrimeric carbohydrate recognition domains, while the collagen region brings about the effector functions via its interaction with cell surface receptors. SP-A and SP-D deal with various pathogens, using a range of innate immune mechanisms such as agglutination/aggregation, enhancement of phagocytosis, and killing mechanisms by phagocytic cells and direct growth inhibition. SP-A and SP-D have also been shown to be involved in the control of pulmonary inflammation including allergy and asthma. Emerging evidence suggest that SP-A and SP-D are capable of linking innate immunity with adaptive immunity that includes modulation of dendritic cell function and helper T cell polarization. This review enumerates immunological properties of SP-A and SP-D inside and outside lungs and discusses their importance in human health and disease. PMID:22701116

Nayak, Annapurna; Dodagatta-Marri, Eswari; Tsolaki, Anthony George; Kishore, Uday

2012-01-01

260

[Cellulase activity of Ceratocystis sp. of different trophic groups].  

PubMed

A comparative analysis of cellulase activity of 36 fungal strains of phytopathogenic and endophytic Ceratocystis sp. was conducted. The rate of their linear growth on the media with carboxymethylcellulose was studied. It was shown that the rate of linear growth of phytopathogenic strains on the media with carboxymethylcellulose was lower than that in endophytic ones. There was no correlation between the levels of cellulase activity of studied strains and rates of their linear growth. The majority of Ceratocystis sp. strains had middle but not high cellulase activity, cellulase activity varied in the group of endophytic strains more than in the phytopathogenic one. The differences in cellulase activity were observed on the strain level. No distinct dependence of cellulase activity on different growth terms of fungi was demonstrated. The distinct dependence of cellulase activity level of endophytes on the species and organs of host plant was not observed. The cellulase activity level of endophytic strains Ceratocystis sp. was lower than in endophytic Fusarium poae strains and similar to it in phytopathogenic Fusarium strains. Consequently the investigated endophytic Ceratocystis sp. strains can be classified as latent pathogens, which were able to cause the diseases of host plants in favorable for them conditions. The paper is presented in Ukrainian. PMID:20455430

Kurchenko, I M; Sokolova, O V; Iur'ieva, O M; Zhdanova, N M

2009-01-01

261

[Xylanase activity of phytopathogenic and endophytic strains of Ceratocystis sp].  

PubMed

A comparative analysis of xylanase activity of 36 phytopathogenic and endophytic Ceratocystis sp. strains was conducted. The rate of their linear growth on the medium with xylan was studied. The rate of linear growth of phytopathogenic strains was 0.003-0.004 mm/h that was almost 70 times less than in endophytic ones. There were no correlation between levels of xylanase activity of studied strains and rates of their linear growth. Xylanase activity ofendophytic Ceratocystis sp. strains varied from complete absence to high level. Phytopathogenic strains possessed only high xylanase activity; maximum values of their xylanase activity zones were three times more than in endophytic strains. The differences in xylanase activity were observed on the strain level. The xylanase activity of 24% endophytic and 64% phytopathogenic strains became higher with increasing of cultivation period. The clear dependence of xylanase activity on the species and organs of host plants was not demonstrated. It was shown that the xylanase activity level of phytopathogenic Ceratocystis sp. strains was too much higher than in such phytopathogens as Fusarium poae, F. oxysporum and Alternaria alternata strains. The conclusion was made that the studied endophytic Ceratocystis sp. strains can be related to latent pathogens, which are able to cause the diseases of host plants in conditions favorable for them. PMID:21117291

Kurchenko, I M; Sokolova, O V; Iur'ieva, O M

2010-01-01

262

Amycolatopsis minnesotensis sp. nov., isolated from a prairie soil  

E-print Network

soil in Minnesota and subjected to characterization by means of polyphasic taxonomy. The 16S rRNA geneAmycolatopsis minnesotensis sp. nov., isolated from a prairie soil Soon Dong Lee,1 Linda L. Kinkel2 in prairie soils with different nitrogen- input histories at the Cedar Creek Natural History Area, an NSF

Minnesota, University of

263

NASA/SP-2007-6105 Systems Engineering  

E-print Network

NASA/SP-2007-6105 Rev1 NASA Systems Engineering Handbook #12;NASA STI Program...in Profile Since its founding, the National Aeronautics and Space Administration (NASA) has been dedicated to the ad- vancement of aeronautics and space science. The NASA Scientific and Technical Information (STI) program

Rhoads, James

264

Soil treatments against Fusarium oxysporum f. sp. vasinfectum race 4  

Technology Transfer Automated Retrieval System (TEKTRAN)

Few economically feasible disease management options are available for California cotton producers with fields infested with race 4 of Fusarium oxysporum f. sp. vasinfectum. For treating soil to reduce inoculum levels, past studies indicate that solarization and fumigation with metam-sodium may be a...

265

Complete Genome Assembly of Corynebacterium sp. Strain ATCC 6931  

PubMed Central

The genus Corynebacterium is best known for the pathogen C. diphtheriae; however, it contains mostly commensal and nonpathogenic, as well as several opportunistic, pathogens. Here, we present the 2.47-Mb scaffolded assembly of the type strain, Corynebacterium sp. ATCC 6931 (NCTC 1914), as deposited into GenBank under accession number CP008913. PMID:25342684

Daligault, H. E.; Davenport, K. W.; Minogue, T. D.; Bishop-Lilly, K. A.; Bruce, D. C.; Chain, P. S.; Coyne, S. R.; Frey, K. G.; Jaissle, J.; Koroleva, G. I.; Ladner, J. T.; Li, P-E.; Meincke, L.; Munk, A. C.; Palacios, G. F.; Redden, C. L.

2014-01-01

266

Sarcocystis jorrini sp. nov. from the fallow deer Cervus dama  

Microsoft Academic Search

Sarcocystis jorrini sp. nov. isolated from the muscle of naturally infected fallow deer (Cervus dama) from the Doñana National Park (Spain) is described by light and electron microscopy. Sarcocysts were macroscopic (2.7×1.4 mm) and were found in all striated muscle, including that of the esophagus and the heart. As visualized by light microscopy, the cyst walls were formed by mammilated

S. Hernández-Rodríguez; I. Acosta; I. Navarrete

1992-01-01

267

WHAT'S GRAPHENE? Mono or few layers of sp2 bonded  

E-print Network

WHAT'S GRAPHENE? · Mono or few layers of sp2 bonded carbon atoms in a honeycomb lattice 105cm2/Vs at RT. 1 Due to its unique transport properties, graphene is suitable for implementation sampling (EOS) timeresolved spectroscopy to optically pump and THz probe exfoliated graphene ribbons (GR

Mellor-Crummey, John

268

Biological effect of the Planktothrix sp. FP1 cyanobacterial extract  

Microsoft Academic Search

Cyanobacteria are common and potentially harmful inhabitants of freshwater and marine environments worldwide. Some waterbloom-forming cyanobacteria are toxic and they may cause animal death and adversely affect human health. A filamentous freshwater cyanobacterium, Planktothrix sp. FP1, was found to be responsible for a toxic algal bloom in Lake Varese (Italy) during August of 1997. In the present study, the biological

M. Prati; M. Molteni; F. Pomati; C. Rossetti; G. Bernardini

2002-01-01

269

Eukiefferiella halvorseni sp. n. from Kashmir (India) (Diptera, Nematocera, Chironomidae)  

Microsoft Academic Search

The male imago of Eukiefferiella halvorseni sp. n. is described from two specimens collected in Kashmir (India). The new species is a member of the E. gracei group. With respect to the hypopygial features it closely resembles E. fittkaui Lehmann, differing primarily by its extremely low AR. Another specimen probably belonging to the same species has been caught in an

N. Caspers

1990-01-01

270

Biscalitheca suzanneana, N. Sp., from the uppermost Pennsylvanian of Texas  

USGS Publications Warehouse

Biscalitheca suzanneana, n. sp., is described from specimens found in an uppermost Pennsylvanian deposit in north-central Texas. The genus was identified primarily on the basis of impressions of the unique sporangial annuli. The occurrence establishes for Biscalitheca a stratigraphic range equivalent to nearly the entire Upper Pennsylvanian Series. ?? 1972.

Mamay, S.H.

1972-01-01

271

Psychromonas japonica sp. nov., Psychromonas aquimarina sp. nov., Psychromonas macrocephali sp. nov. and Psychromonas ossibalaenae sp. nov., psychrotrophic bacteria isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan.  

PubMed

Four novel species of psychrotrophic bacteria were isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of 228-250 m. Cells of the five isolated strains, JAMM 0394(T), JAMM 0404(T), JAMM 0415(T), JAMM 0700 and JAMM 0738(T), were Gram-negative, rod-shaped, non-sporulating and motile by means of a single polar flagellum. The novel strains were able to produce isoprenoid quinone Q-8 as the major component. The predominant fatty acids were C(16 : 0) and C(16 : 1). Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strains represent a separate lineage within the genus Psychromonas. The G+C contents of the novel strains were 38.8-42.8 mol%. DNA-DNA hybridization values between these strains and reference strains of the genus Psychromonas were significantly lower than that generally accepted as the threshold for the phylogenetic definition of species. The names Psychromonas japonica sp. nov. (type strain JAMM 0394(T)=JCM 14783(T)=ATCC BAA-1525(T)), Psychromonas aquimarina sp. nov. (type strain JAMM 0404(T)=JCM 14784(T)=ATCC BAA-1526(T)), Psychromonas macrocephali sp. nov. (two strains, type strain JAMM 0415(T)=JCM 14785(T)=ATCC BAA-1527(T)) and Psychromonas ossibalaenae sp. nov. (type strain JAMM 0738(T)=JCM 14786(T)=ATCC BAA-1528(T)) are proposed. PMID:18599721

Miyazaki, Masayuki; Nogi, Yuichi; Fujiwara, Yoshihiro; Horikoshi, Koki

2008-07-01

272

Leucobacter salsicius sp. nov., from a salt-fermented food  

E-print Network

salt-fermented seafood. Shrimp jeotgal is made by mixing fresh tiny shrimps with rock salt, followedLeucobacter salsicius sp. nov., from a salt- fermented food Ji-Hyun Yun,1 Seong Woon Roh,1,2 Min, Daejeon 305-806, Republic of Korea Strain M1-8T was isolated from jeotgal, a Korean salt-fermented food

Bae, Jin-Woo

273

cDNAs from Nylanderia sp nr pubens (Hymenoptera: Formicidae)  

Technology Transfer Automated Retrieval System (TEKTRAN)

7 new gene sequences were identified from workers of Rasberry crazy ant, Nylanderia sp.nr. pubens, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are HQ636472-HQ636478. This information will provide scientists with genetic tools to study the pop...

274

THE SAGA OF A MALE FERTILITY PROTEIN (SP22)  

EPA Science Inventory

Toxicologic studies designed to identify chemical-induced alterations in the structure and function of the epididymis, particularly the acquisition of fertility by proximal cauda epididymal sperm, have lead to the discovery of a novel sperm protein (SP22) that is well correlated ...

275

Pandoraea sp. RB-44, a novel quorum sensing soil bacterium.  

PubMed

Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various chain lengths and degrees of saturation at the C-3 position. We have isolated a bacterium, RB-44, from a site which was formally a landfill dumping ground. Using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis, this isolate was identified as a Pandoraea sp.which was then screened for AHL production using biosensors which indicated its quorum sensing properties. To identify the AHL profile of Pandoraea sp. RB-44, we used high resolution tandem mass spectrometry confirming that this isolate produced N-octanoylhomoserine lactone (C8-HSL). To the best of our knowledge, this is the first report that showed quorum sensing activity exhibited by Pandoraea sp. Our data add Pandoraea sp. to the growing number of bacteria that possess QS systems. PMID:24145919

Han-Jen, Robson Ee; Wai-Fong, Yin; Kok-Gan, Chan

2013-01-01

276

Blastopirellula cremea sp. nov., isolated from a dead ark clam  

E-print Network

Blastopirellula cremea sp. nov., isolated from a dead ark clam Hae-Won Lee,1, 2 3 Seong Woon Roh,1 a dead ark clam (Scapharca broughtonii) from the south coast of Korea. Strain LHWP2T grew optimally at 30 clam farm in Gangjin Bay, South Korea. In this study, the novel strain LHWP2T was characterized using

Bae, Jin-Woo

277

Complete Genome Sequence of Carnobacterium sp. 17-4?  

PubMed Central

Members of the carnobacteria have been extensively studied as probiotic cultures in aquacultures and protective cultures in seafood, diary, and meat. We report on the finished genome sequence of Carnobacterium sp. 17-4, which has been isolated from permanently cold seawater. The genetic information reveals a new circular bacteriocin biosynthesis cluster. PMID:21551290

Voget, Sonja; Klippel, Barbara; Daniel, Rolf; Antranikian, Garabed

2011-01-01

278

Civilian and military missions SP-100 preliminary user requirements  

SciTech Connect

This document defines the top level requirements of potential users of a space based nuclear electric power supply. This provides the SP-100 Project and information required to design the modular (10-1000 KWe) space power systems to meet the needs of most potential users.

Not Available

1987-06-29

279

An HPF compiler for the IBM SP2  

Microsoft Academic Search

We describe pHPF, an research prototype HPF compiler for the IBM SP series parallel machines. The compiler accepts as input Fortran 90 and Fortran 77 programs, augmented with HPF directives; sequential loops are automatically parallelized. The compiler supports symbolic analysis of expressions. This allows parameters such as the number of processors to be unknown at compile-time without significantly affecting performance.

Manish Gupta; Samuel P. Midkiff; Edith Schonberg; Ven Seshadri; David Shields; Ko-Yang Wang; Wai-Mee Ching; Ton Ngo

1995-01-01

280

SP100 early demonstration flight system design and testing  

Microsoft Academic Search

A Space Nuclear Power System design for an early flight application has been developed focused on SP-100 technology currently available. This 10 kWe class space power plant uses a fast spectrum Li cooled reactor coupled to the highly successful thermoelectric converters which have flown on the Galileo and Ulysses missions. The concept is characterized by low mass, high reliability, and

Neil W. Brown; Hwang Choe; Stanley M. Davies; Aaron S. Kirpich

1993-01-01

281

SP100, a flexible technology for space power  

Microsoft Academic Search

Power requirements for the ambitious space missions of the future exceed the limits of current power system availability. Space reactor power meets this need. The US is developing space reactor power system technology to meet the projected civilian and defense needs for the 1990s and beyond. This program, known as SP-100, will remove the restrictions on electrical power generation that

M. A. Smith; J. D. Stephen; S. L. Stewart; Hwang Cho; A. Kirpich; N. F. Shepard

1989-01-01

282

Testing of SP100 reactor control approaches in the NAT  

Microsoft Academic Search

A control scheme has been developed for the Nuclear Assembly Test (NAT) program to emulate the generic flight system (GFS) reactor control approach in the case of the SP-100 ground engineering system. Comparisons between the GFS simulation results and those of the NAT, giving attention to the reactor inlet flow rate and temperature, indicate that a reasonbly close simulation of

Sang K. Rhow; Raymond A. Meyer; Kwok K. Wong; Frank J. Halfen

1991-01-01

283

SP100 lithium thaw design, analysis, and testing  

Microsoft Academic Search

The thaw design has been established for the 100 kWe SP-100 Space Reactor Power System. System thaw\\/startup analysis has confirmed that all system thaw requirements are met, and that rethaw and restart can be easily accomplished with this design. In addition, a series of lithium thaw characterization tests has been performed, confirming key design assumptions.

Hwang Choe; Michael R. Schrag; David R. Koonce; Robert E. Gamble; Frank J. Halfen; Aaron S. Kirpich

1993-01-01

284

Fabrication of the SP100 Thermoelectric-Electromagnetic Pump  

Microsoft Academic Search

The thermoelectric electromagnetic (TEM) pump circulates lithium in the primary and secondary coolant circuits of the SP-100 Space Power Reactor System. Because of the high temperature of the coolant the pump is made primarily from niobium - 1 percent zirconium (Nb-1%Zr) alloy and is self-powered by semiconductor thermoelectric cells. Design features needed to optimize pumping efficiency such as bonded dissimilar

Robert E. Butler; Carlos Martinez; Thomas J. Ruffo; Edwin D. Sayre

1994-01-01

285

SP100 Interplanetary NEP and lunar surface power applications  

Microsoft Academic Search

This paper describes how the SP-100 Space Reactor Power System (SRPS) can be tailored to meet the specific requirements for a lunar surface power system to meet the needs of the consolidation and utilization phases outlined in the 90-day NASA SEI study report. This same basic power system can also be configured to obtain the low specific masses needed to

A. T. Josloff; J. S. Armijo; D. N. Matteo; H. S. Bailey; N. Brown; P. Pluta; M. A. Smith; N. F. Shepard; J. D. Stephen

1992-01-01

286

Testing of SP100 reactor control approaches in the NAT  

Microsoft Academic Search

The Generic Flight System (GFS) reactor control approach will be tested at the SP-100 Ground Engineering System (GES) test site as part of the Nuclear Assembly Test (NAT) program. A control scheme for the NAT has been developed to emulate the GFS conditions for testing of the GFS reactor control approach in the NAT. Comparisons between the GFS simulation results

Sang K. Rhow; Raymond A. Meyer; Kwok K. Wong; Frank J. Halfen

1991-01-01

287

Process Engineering Thermodynamics RZ 424304 E (4 sp) 2013  

E-print Network

. Calculate the exergy losses. For -Al2O3 (corundum) the standard Gibbs energy of formation is fG298 = -1582Process Engineering Thermodynamics RZ 424304 E (4 sp) 2013 Exercises 3+4 of 4 12.2+14.2.2013 Note: Questions 7 and 10/401 after lecture "Irreversible thermodynamics" 1. (S05) Calculate the exergy losses

Zevenhoven, Ron

288

DEATH AND AUTHENTICITY PHI 500/REL 600, SP. 2010  

E-print Network

DEATH AND AUTHENTICITY PHI 500/REL 600, SP. 2010 PROF. ED MOONEY, RELIGION AND PHILOSOPHY HL 501, M to ourselves, doing what we must) and death (its nothingness, its threats). For evoking and unraveling these enigmas and puzzles we can't do better than start with Tolstoy's short story, "The Death of Ivan Ilych

Kovalev, Leonid

289

Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter  

E-print Network

Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter Na-Ri Shin, Mi , was isolated from a Korean alcohol fermentation starter. Optimal growth occurred at 37 6C, at pH 8 and in 1 was isolated from nuruk, which is an alcohol fermentation starter used as an amylolytic substance

Bae, Jin-Woo

290

Uranium accumulation by immobilized cells of a Citrobacter sp  

Microsoft Academic Search

Uranium was removed from challenge flows presented to immobilized cells of aCitrobacter sp. In excess of 90% of the presented metal was recovered, giving high yields of accumulated metal which could be subsequently released from the immobilized cellsin situ.

Lynne E. Macaskie; A. C. R. Dean

1985-01-01

291

Mice deficient in surfactant protein A (SP-A) and SP-D or in TLR2 manifest delayed parturition and decreased expression of inflammatory and contractile genes.  

PubMed

Previously we obtained compelling evidence that the fetus provides a critical signal for the initiation of term labor through developmental induction of surfactant protein (SP)-A expression by the fetal lung and secretion into amniotic fluid (AF). We proposed that interactions of AF macrophage (M?) Toll-like receptors (TLRs) with SP-A, at term, or bacterial components, at preterm, result in their activation and migration to the pregnant uterus. Herein the timing of labor in wild-type (WT) C57BL/6 mice was compared with mice homozygous null for TLR2, SP-A, SP-D, or doubly deficient in SP-A and SP-D. Interestingly, TLR2(-/-) females manifested a significant (P < 0.001) delay in timing of labor compared with WT as well as reduced expression of the myometrial contraction-associated protein (CAP) gene, connexin-43, and M? marker, F4/80, at 18.5 d postcoitum (dpc). Whereas in first pregnancies, SP-A(-/-), SP-D(-/-), and SP-A/D(-/-) females delivered at term (?19.5 dpc), in second pregnancies, parturition was delayed by approximately 12 h in SP-A(-/-) (P = 0.07) and in SP-A/D(-/-) (P <0.001) females. Myometrium of SP-A/D(-/-) females expressed significantly lower levels of IL-1?, IL-6, and CAP genes, connexin-43, and oxytocin receptor at 18.5 dpc compared with WT. F4/80(+) AF M?s from TLR2(-/-) and SP-A/D(-/-) mice expressed significantly lower levels of both proinflammatory and antiinflammatory activation markers (e.g. IL-1?, IL-6, ARG1, YM1) compared with gestation-matched WT AF M?s. These novel findings suggest that the pulmonary collectins acting via TLR2 serve a modulatory role in the timing of labor; their relative impact may be dependent on parity. PMID:23183169

Montalbano, Alina P; Hawgood, Samuel; Mendelson, Carole R

2013-01-01

292

Nome do aluno(a) Curso do aluno Cidade/Estado Douglas Anderson dos Santos Filosofia So Paulo/SP  

E-print Network

Engenharia de Software São Paulo/SP Thiago Oliveira Carvalho Quimica São Pedro/SP Rayssa Bruzaca de Andrade Fisica São Paulo/SP Riane Ribeiro Carvalho Quimica São Paulo/SP Caroline Luchtenberg Ribeiro Quimica São Pereira dos Santos Quimica São Paulo/SP Paula Marcelly Alves Machado Quimica São Paulo/SP Rosylane Elaine

Lucero, Jorge Carlos

293

The sea urchin complement homologue, SpC3, functions as an opsonin  

Microsoft Academic Search

The purple sea urchin Strongylocentrotus purpuratus expresses a homologue of complement component C3 (SpC3), which acts as a humoral opsonin. Significantly increased phagocytic activity was evident when yeast target cells were opsonized after incubation with coelomic fluid containing SpC3. SpC3 could be detected on the surface of yeast, and phagocytic activity could be inhibited by an anti-SpC3 antibody. This indicates

Lori A. Clow; David A. Raftos; Paul S. Gross; L. Courtney Smith

2004-01-01

294

Biotransformation of industrial reactive azo dyes by Geotrichum sp. CCMI 1019  

Microsoft Academic Search

Geotrichum sp. was selected from among several fungi because of its ability to transform three reactive azo dyes (Reactive Black 5, Reactive Red 158 and Reactive Yellow 27) used in industry. None of the white rot fungi tested (Bjerkandera adusta, Ganoderma sp., Irpex lacteus, Phanerochaete magnoliae, Rigidoporus sp., and Trametes versicolor) were able to transform the yellow and red dyes,

Cristina Máximo; Maria T. Pessoa Amorim; Maria Costa-Ferreira

2003-01-01

295

Report to TechWise Preliminary report Sandeels and clams (Spisula sp.)  

E-print Network

Report to TechWise Preliminary report April 2003 Sandeels and clams (Spisula sp.) in the wind. Summary and conclusions 03 1.1 Sandeels 03 1.2 Clams (Spisula sp.) 03 1.3 Other animals 04 2. Purpose surveys 23 4.3 Clams (Spisula sp.), biology and possible impact 32 4.3.1. Results from surveys 32 4

296

Draft Genome Sequence of Rhodovulum sp. Strain NI22, a Naphthalene-Degrading Marine Bacterium.  

PubMed

Rhodovulum sp. strain NI22 is a hydrocarbon-degrading member of the genus Rhodovulum. The draft genome of Rhodovulum sp. NI22 is 3.8 Mb in size, with 3,756 coding sequences and 64.4% G+C content. The catechol and gentisate pathways for naphthalene degradation are predicted to be present in Rhodovulum sp. NI22. PMID:25614575

Brown, Lisa M; Gunasekera, Thusitha S; Bowen, Loryn L; Ruiz, Oscar N

2015-01-01

297

IDENTIFICATION AND MOLECULAR CLONING OF XENOPUS LAEVIS SP22, A PROTEIN ASSOCIATED WITH FERTILIZATION IN MAMMALS  

EPA Science Inventory

ABSTRACT SP22 is a protein that has been characterized in rats where it has been related with fertility. SP22 homologues have been studied in mouse and man and a definitive role for the protein has not been assigned yet. By means of a polyclonal IgG to recombinant rat SP22...

298

Neotobrilus nicsmolae n. sp. (Tobrilidae: Nematoda) and Chronogaster carolinensis n. sp. (Chronogasteridae: Nematoda) from Lake Phelps, North Carolina.  

PubMed

Two new species, Neotobrilus nicsmolae n. sp. and Chronogaster carolinensis n. sp. are described from a small, acidic, temperate, natural lake in North Carolina. N. nicsmolae n. sp. comes close to three members of the genus reported from North America, N. filipjevi, N. longus, and N. hopei. However, N. nicsmolae is unique with in the genus in having a combination of characters: size smaller than 1,700 ?m, shorter outer labial and cephalic setae, tail shorter than 250 ?m, last ventromedian supplement close (about 5 ?m) to cloacal opening, spicule length of 61 to 85 ?m, flagelloid sperm, and possession of subterminal setae. Assessment of relationships among clades within the Triplonchida using DNA sequences of the D2D3 expansion segment of the LSU rDNA showed that the family Trichodoridae and the genus Tripyla were recovered as monophyletic. The genus Tobrilus was recovered as monophyletic in the neighbor-joining and maximum likelihood trees, but that was not so in the maximum-parsimony tree. The separation among genera of the Trichodoridae, i.e., Trichodorus and Paratrichodorus, was not clear-cut in all phylograms. Chronogaster carolinensis n. sp. in having one ventral mucro with no spine and vacuolated lateral glandular bodies comes close to C. typica and C. ethiopica but differs from all hitherto known species in a combination of characteristics: in having long cephalic setae, long stoma, crystalloid bodies, vacuolated lateral glandular bodies, and a tail terminus with blunt ventral mucro, and its lack of lateral line. PMID:23589662

Abebe, Eyualem; Ferebee, Briana; Taylor, Tarreyca; Mundo-Ocampo, Manuel; Mekete, Tesfamariam; De Ley, Paul

2013-03-01

299

Synthesis of pyrrolophenanthridine alkaloids based on C(sp³)-H and C(sp²)-H functionalization reactions.  

PubMed

Assoanine, pratosine, hippadine, and dehydroanhydrolycorine belong to the pyrrolophenanthridine family of alkaloids, which are isolated from plants of the Amaryllidaceae species. Structurally, these alkaloids are characterized by a tetracyclic skeleton that contains a biaryl moiety and an indole core, and compounds belonging to this class have received considerable interest from researchers in a number of fields because of their biological properties and the challenges associated with their synthesis. Herein, a strategy for the total synthesis of these alkaloids by using C-H activation chemistry is described. The tetracyclic skeleton was constructed in a stepwise manner by C(sp(3))-H functionalization followed by a Catellani reaction, including C(sp(2))-H functionalization. A one-pot reaction involving both C(sp(3))-H and C(sp(2))-H functionalization was also attempted. This newly developed strategy is suitable for the facile preparation of various analogues because it uses simple starting materials and does not require protecting groups. PMID:25044314

Tsukano, Chihiro; Muto, Nobusuke; Enkhtaivan, Iderbat; Takemoto, Yoshiji

2014-09-01

300

Digenetic trematodes, Acanthatrium sp. and Lecithodendrium sp., as vectors of Neorickettsia risticii, the agent of Potomac horse fever.  

PubMed

Neorickettsia (formerly Ehrlichia) risticii, the agent of Potomac horse fever (PHF), has been recently detected in trematode stages found in the secretions of freshwater snails and in aquatic insects. Insectivores, such as bats and birds, may serve as the definitive host of the trematode vector. To determine the definitive helminth vector, five bats (Myotis yumanensis) and three swallows (Hirundo rustica, Tachycineta bicolor) were collected from a PHF endemic location in northern California. Bats and swallows were dissected and their major organs examined for trematodes and for N. risticii DNA using a nested polymerase chain reaction (PCR) assay. Adult digenetic trematodes, Acanthatrium sp. and/or Lecithodendrium sp., were recovered from the gastrointestinal tract of all bats and from one swallow. The intestine of three bats, the spleen of two bats and one swallow as well as the liver of one swallow tested PCR positive for N. risticii. From a total of seven pools of identical digenetic trematodes collected from single hosts, two pools of Acanthatrium sp. and one pool of Lecithodendrium sp. tested PCR positive. The results of this investigation provide preliminary evidence that at least two trematodes in the family Lecithodendriidae are vectors of N. risticii. The data also suggest that bats and swallows not only act as a host for trematodes but also as a possible natural reservoir for N. risticii. PMID:14627451

Pusterla, N; Johnson, E M; Chae, J S; Madigan, J E

2003-12-01

301

Characterization and host range of the symbiotic fungus Fusarium euwallaceae sp. nov., vectored by the invasive ambrosia beetle Euwallacea sp.  

Technology Transfer Automated Retrieval System (TEKTRAN)

A novel symbiotic Fusarium euwallaceae fungus that serves as a specific nutritional source for the invasive Asian ambrosia beetle Euwallacea sp. (Coleoptera, Scolytinae, Xyleborini) is farmed in the galleries of host plants. This beetle-fungus complex, which has invaded Israel and California, is clo...

302

INTERACTION BETWEEN AN AMMONIUM-OXIDIZER, 'NITROSOMONAS' SP., AND TWO HETEROTROPHIC BACTERIA, 'NOCARDIA ATLANTICA' AND 'PSEUDOMONAS' SP.: A NOTE  

EPA Science Inventory

Closed culture experiments showed that the ability of an estuarine chemolithotrophic ammonium-oxidizer, Nitrosomonas sp., to convert ammonium to nitrite was substantially increased by as much as 150% and 50% when grown in the presence of two heterotrophs isolated from the same en...

303

Mixibius parvus sp. nov. and Diphascon (Diphascon) ziliense sp. nov., two new species of Eutardigrada from Sicily.  

PubMed

Two new species, Mixibius parvus sp. nov. and Diphascon (Diphascon) ziliense sp. nov. are described from Sicily. Mixibius parvus sp. nov. has three macroplacoids and a microplacoid and differs from M. tibetanus, the only other known species of the genus with those characteristics, in having a wrinkled cuticle without true small tubercles, a shorter microplacoid, smaller claw pt index values, and in lacking a cuticular bar on the first three pairs of the legs.Diphascon (Diphascon) ziliense sp. nov. lacks eye spots, has a pharyngeal bulb with two macroplacoids and a septulum, and possesses lunules and cuticular bars on the legs. It is similar to D. (D.) ramazzottii and D. (D.) procerum but differs from them in characters of the cuticular ornamentation and, in addition, from D. (D.) ramazzottii in having lunules and slightly longer claws in proportion to the body size and to the buccal tube. The new species differs from D. (D.) procerum in having stouter claws with a wider common portion and with the main branches shorter in proportion to the total length of the respective claws. PMID:24871024

Lisi, Oscar; Sabella, Giorgio; Pilato, Giovanni

2014-01-01

304

Neotobrilus nicsmolae n. sp. (Tobrilidae: Nematoda) and Chronogaster carolinensis n. sp. (Chronogasteridae: Nematoda) from Lake Phelps, North Carolina  

PubMed Central

Two new species, Neotobrilus nicsmolae n. sp. and Chronogaster carolinensis n. sp. are described from a small, acidic, temperate, natural lake in North Carolina. N. nicsmolae n. sp. comes close to three members of the genus reported from North America, N. filipjevi, N. longus, and N. hopei. However, N. nicsmolae is unique with in the genus in having a combination of characters: size smaller than 1,700 ?m, shorter outer labial and cephalic setae, tail shorter than 250 ?m, last ventromedian supplement close (about 5 ?m) to cloacal opening, spicule length of 61 to 85 ?m, flagelloid sperm, and possession of subterminal setae. Assessment of relationships among clades within the Triplonchida using DNA sequences of the D2D3 expansion segment of the LSU rDNA showed that the family Trichodoridae and the genus Tripyla were recovered as monophyletic. The genus Tobrilus was recovered as monophyletic in the neighbor-joining and maximum likelihood trees, but that was not so in the maximum-parsimony tree. The separation among genera of the Trichodoridae, i.e., Trichodorus and Paratrichodorus, was not clear-cut in all phylograms. Chronogaster carolinensis n. sp. in having one ventral mucro with no spine and vacuolated lateral glandular bodies comes close to C. typica and C. ethiopica but differs from all hitherto known species in a combination of characteristics: in having long cephalic setae, long stoma, crystalloid bodies, vacuolated lateral glandular bodies, and a tail terminus with blunt ventral mucro, and its lack of lateral line. PMID:23589662

Abebe, Eyualem; Ferebee, Briana; Taylor, Tarreyca; Mundo-Ocampo, Manuel; Mekete, Tesfamariam; De Ley, Paul

2013-01-01

305

FEBS Lett . Author manuscript Fluctuation of the SP/non-SP phenotype in the C6 glioma cell line  

E-print Network

cell line Nadine Platet 1 , Jean-Fran ois Mayolç 2 , Fran ois Bergerç 1 , Francis H rodiné 2 , Didier ; Cell Line, Tumor ; metabolism ; pathology ; Cell Transformation, Neoplastic ; metabolism ; pathology evidence for the existence of a distinct SP in tumor cell lines such as C6, U373, MCF7, HeLa and many other

Paris-Sud XI, Université de

306

Halobellus limi sp. nov. and Halobellus salinus sp. nov., isolated from two marine solar salterns.  

PubMed

Two halophilic archaea, strains TBN53(T) and CSW2.24.4(T), were characterized to elucidate their taxonomic status. Strain TBN53(T) was isolated from the Taibei marine solar saltern near Lianyungang city, Jiangsu province, China, whereas strain CSW2.24.4(T) was isolated from a saltern crystallizer in Victoria, Australia. Cells of the two strains were pleomorphic, stained Gram-negative and produced red-pigmented colonies. Strain TBN53(T) was able to grow at 25-55 °C (optimum 45 °C), with 1.4-5.1 M NaCl (optimum 2.6-3.9 M NaCl), with 0-1.0 M MgCl(2) (optimum 0-0.1 M MgCl(2)) and at pH 5.5-9.5 (optimum pH 7.0), whereas strain CSW2.24.4(T) was able to grow at 25-45 °C (optimum 37 °C), with 2.6-5.1 M NaCl (optimum 3.4 M NaCl), with 0.01-0.7 M MgCl(2) (optimum 0.05 M MgCl(2)) and at pH 5.5-9.5 (optimum pH 7.0-7.5). Cells of the two isolates lysed in distilled water. The minimum NaCl concentrations that prevented cell lysis were 8 % (w/v) for strain TBN53(T) and 12 % (w/v) for strain CSW2.24.4(T). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate, with two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. Trace amounts of other unidentified lipids were also detected. On the basis of 16S rRNA gene sequence analysis, strains TBN53(T) and CSW2.24.4(T) showed 94.1 % similarity to each other and were closely related to Halobellus clavatus TNN18(T) (95.0 and 94.7 % similarity, respectively). Levels of rpoB' gene sequence similarity between strains TBN53(T) and CSW2.24.4(T), and between these strains and Halobellus clavatus TNN18(T) were 88.5, 88.5 and 88.1 %, respectively. The DNA G+C contents of strains TBN53(T) and CSW2.24.4(T) were 69.2 and 67.0 mol%, respectively. The level of DNA-DNA relatedness between strain TBN53(T) and strain CSW2.24.4(T) was 25 %, and these two strains showed low levels of DNA-DNA relatedness with Halobellus clavatus TNN18(T) (30 and 29 % relatedness, respectively). Based on these phenotypic, chemotaxonomic and phylogenetic properties, two novel species of the genus Halobellus are proposed to accommodate these two strains, Halobellus limi sp. nov. (type strain TBN53(T) = CGMCC 1.10331(T) = JCM 16811(T)) and Halobellus salinus sp. nov. (type strain CSW2.24.4(T) = DSM 18730(T) = CGMCC 1.10710(T) = JCM 14359(T)). PMID:22661071

Cui, Heng-Lin; Yang, Xin; Zhou, Yu-Guang; Liu, Hong-Can; Zhou, Pei-Jin; Dyall-Smith, Mike L

2012-06-01

307

Identification and Characterization of Spontaneous Deletions within the Sp11-Sp12 Prophage Region of Escherichia coli O157:H7 Sakai  

PubMed Central

Prophages make up 12% of the enterohemorrhagic Escherichia coli genome and play prominent roles in the evolution and virulence of this food-borne pathogen. Acquisition and loss of and rearrangements within prophage regions are the primary causes of differences in pulsed-field gel electrophoresis (PFGE) patterns among strains of E. coli O157:H7. Sp11 and Sp12 are two tandemly integrated and putatively defective prophages carried by E. coli O157:H7 strain Sakai. In this study, we identified 3 classes of deletions that occur within the Sp11-Sp12 region, at a frequency of ca. 7.74 × 10?4. One deletion resulted in a precise excision of Sp11, and the other two spanned the junction of Sp11 and Sp12. All deletions resulted in shifts in the XbaI fragment pattern observed by PFGE. We sequenced the inducible prophage pool of Sakai but did not identify any mature phage particles corresponding to either Sp11 or Sp12. Deletions containing pchB and psrC, which are Sp11-carried genes encoding proteins known or suspected to regulate type III secretion, did not affect the secretion levels of the EspA or EspB effector. Alignment of the Sp11-Sp12 DNA sequence with its corresponding regions in other E. coli O157:H7 and O55:H7 strains suggested that homologous recombination rather than integrase-mediated excision is the mechanism behind these deletions. Therefore, this study provides a mechanism behind the previously observed genetic instability of this genomic region of E. coli O157:H7. PMID:23315730

Chen, Chun; Lewis, Carrie R.; Goswami, Kakolie; Roberts, Elisabeth L.; DebRoy, Chitrita

2013-01-01

308

Cobryketone derived from vitamin B12 via palladium-catalyzed cleavage of the sp3-sp3 carbon-carbon bond.  

PubMed

Heptamethyl cobyrinate was transformed into hexamethyl 8-nor-cobyrinate. The crucial step involved the synthesis of new, vitamin B12 derived cobryketone via palladium-catalyzed cleavage of the sp(3)-sp(3) carbon-carbon bond with the liberation of the ketone. The replacement of sp(3) carbon atom with sp(2) (C?O) at the 8-position produces a bathochromic shift of all absorption bands and makes ? and ? bands equal as a consequence of the expansion of the existing conjugated system of double bonds. PMID:23544362

Kurco?, Sylwester; Proinsias, Keith ó; Gryko, Dorota

2013-04-19

309

Candida kashinagacola sp. nov., C. pseudovanderkliftii sp. nov. and C. vanderkliftii sp. nov., three new yeasts from ambrosia beetle-associated sources.  

PubMed

Three new yeast species, Candida kashinagacola (JCM 15019(T) = CBS 10903(T)), C. pseudovanderkliftii (JCM 15025(T) = CBS 10904(T)), and C. vanderkliftii (JCM 15029(T) = CBS 10905(T)) are described on the basis of comparison of nucleotide sequences of large subunit ribosomal DNA D1/D2 region (LSU rDNA D1/D2). The nearest assigned species of the three new species was Candida llanquihuensis. Candida kashinagacola and C. pseudovanderkliftii differed from C. llanquihuensis by 3.8% nucleotide substitution of the region, while C. vanderkliftii did by 4.4%. Three new species differed in a number of physiological and growth characteristics from any previously assigned species and from one another. A phylogenetic tree based on the sequences of LSU rDNA D1/D2 showed that these new species together with Candida sp. ST-246, Candida sp. JW01-7-11-1-4-y2, Candida sp. BG02-7-20-001A-2-1 and C. llanquihuensis form a clade near Ambrosiozyma species. The new species did not assimilate methanol as a sole source of carbon, which supported the monophyly of these non methanol-assimilating species which are closely related to the methylotrophic yeasts. Candida kashinagacola was frequently isolated from the beetle galleries of Platypus quercivorus in three different host trees (Quercus serrata, Q. laurifolia and Castanopsis cuspidata) located in the sourthern part of Kyoto, Japan, thus indicating that this species may be a primary ambrosia fungus of P. quercivorus. On the other hand, C. pseudovanderkliftii and C. vanderkliftii were isolated only from beetle galleries in Q. laurifolia. Candida vanderkliftii was isolated from beetle gallery of Platypus lewisi as well as those of P. quercivorus. Candida pseudovanderkliftii and C. vanderkliftii are assumed to be auxiliary ambrosia fungi of P. quercivorus. PMID:18537038

Endoh, Rikiya; Suzuki, Motofumi; Benno, Yoshimi; Futai, Kazuyoshi

2008-10-01

310

NSS5/SP-STM2 Joint International Conference  

SciTech Connect

The NSS5/SP-STM2 conference was held in Athens, Ohio July 15-19, 2008. The conference brought together a prestigious group of scientists from all over the globe to focus for 3 ½ days on a variety of nanoscience topics, particularly on nanoscale spectroscopy and spin-polarized scanning tunneling microscopy. The conference was attended by many young scientists as well as senior scientists. Attendees to the conference were drawn from more than 10 countries and included 28 invited speakers, who are the leading scientists in their respective research areas. Included among the invited speakers were 4 plenary speakers - eminent scientists in their fields. The conference was divided into two parallel sessions – the NSS5 session and the SP-STM2 session.

Saw-Wai Hla

2009-05-03

311

Arachnomyces kanei (anamorph Onychocola kanei) sp. nov., from human nails.  

PubMed

Five isolates of a slow-growing cycloheximide resistant hyphomycetous fungus were obtained from nail specimens and investigated for their relationship to Onychocola canadensis (teleomorph Arachnomyces nodosetosus), a known agent of onychomycosis. In one patient diagnosed with superficial white onychomycosis, etiology was confirmed by a nail sample showing atypical filaments in direct microscopy, and by a follow-up specimen yielding cultures of the same fungus. A case of mixed infection with Aspergillus sydowii was also confirmed after examination of cultures grown from three successive microscopic-positive hallux nail specimens. For other isolates, etiological significance could not be confirmed by repeat sampling or results of direct microscopy were negative or unknown. Mating experiments yielded setose ascomata containing smooth oblate ascospores typical of Arachnomyces species. Phylogenetic analysis of ITS 2 region sequences support the conspecificity of the isolates and their placement within the genus. A. kanei sp. nov. (anamorph O. kanei sp. nov.) is described. PMID:12521121

Gibas, C F C; Sigler, L; Summerbell, R C; Hofstader, S L R; Gupta, A K

2002-12-01

312

Complete genome sequence of Paenibacillus sp. strain JDR-2  

SciTech Connect

Paenibacillus sp. strain JDR-2, an aggressively xylanolytic bacterium isolated from sweetgum (Liquidambar styraciflua) wood, is able to efficiently depolymerize, assimilate and metabolize 4-O-methylglucuronoxylan, the predominant structural component of hardwood hemicelluloses. A basis for this capability was first supported by the identification of genes and characterization of encoded enzymes and has been further defined by the sequencing and annotation of the complete genome, which we describe. In addition to genes implicated in the utilization of -1,4-xylan, genes have also been identified for the utilization of other hemicellulosic polysaccharides. The genome of Paenibacillus sp. JDR-2 contains 7,184,930 bp in a single replicon with 6,288 protein-coding and 122 RNA genes. Uniquely prominent are 874 genes encoding proteins involved in carbohydrate transport and metabolism. The prevalence and organization of these genes support a metabolic potential for bioprocessing of hemicellulose fractions derived from lignocellulosic resources.

Chow, Virginia [University of Florida; Nong, Guang [University of Florida; St. John, Franz J. [US Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA; Dickstein, Ellen [University of Florida; Chertkov, Olga [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Brettin, Thomas S [ORNL; Han, James [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Martin, Joel [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Jones, Jeffrey B. [University of Florida; Ingram, Lonnie O. [University of Florida; Shanmugam, Keelnathan T. [University of Florida; Preston, James F. [University of Florida

2012-01-01

313

Tongue adhesion in the horned frog Ceratophrys sp.  

NASA Astrophysics Data System (ADS)

Frogs are well-known to capture elusive prey with their protrusible and adhesive tongues. However, the adhesive performance of frog tongues and the mechanism of the contact formation with the prey item remain unknown. Here we measured for the first time adhesive forces and tongue contact areas in living individuals of a horned frog (Ceratophrys sp.) against glass. We found that Ceratophrys sp. generates adhesive forces well beyond its own body weight. Surprisingly, we found that the tongues adhered stronger in feeding trials in which the coverage of the tongue contact area with mucus was relatively low. Thus, besides the presence of mucus, other features of the frog tongue (surface profile, material properties) are important to generate sufficient adhesive forces. Overall, the experimental data shows that frog tongues can be best compared to pressure sensitive adhesives (PSAs) that are of common technical use as adhesive tapes or labels.

Kleinteich, Thomas; Gorb, Stanislav N.

2014-06-01

314

Complete genome sequence of Paenibacillus sp. strain JDR-2  

PubMed Central

Paenibacillus sp. strain JDR-2, an aggressively xylanolytic bacterium isolated from sweetgum (Liquidambar styraciflua) wood, is able to efficiently depolymerize, assimilate and metabolize 4-O-methylglucuronoxylan, the predominant structural component of hardwood hemicelluloses. A basis for this capability was first supported by the identification of genes and characterization of encoded enzymes and has been further defined by the sequencing and annotation of the complete genome, which we describe. In addition to genes implicated in the utilization of ?-1,4-xylan, genes have also been identified for the utilization of other hemicellulosic polysaccharides. The genome of Paenibacillus sp. JDR-2 contains 7,184,930 bp in a single replicon with 6,288 protein-coding and 122 RNA genes. Uniquely prominent are 874 genes encoding proteins involved in carbohydrate transport and metabolism. The prevalence and organization of these genes support a metabolic potential for bioprocessing of hemicellulose fractions derived from lignocellulosic resources. PMID:22675593

Chow, Virginia; Nong, Guang; St. John, Franz J.; Rice, John D.; Dickstein, Ellen; Chertkov, Olga; Bruce, David; Detter, Chris; Brettin, Thomas; Han, James; Woyke, Tanja; Pitluck, Sam; Nolan, Matt; Pati, Amrita; Martin, Joel; Copeland, Alex; Land, Miriam L.; Goodwin, Lynne; Jones, Jeffrey B.; Ingram, Lonnie O.; Shanmugam, Keelnathan T.; Preston, James F.

2012-01-01

315

Evaluation on clot buster activity of staphylokinase from Staphylococcus sp.  

PubMed

Thrombolytic agents play a major role in the treatment of cardiovascular diseases. Presently streptokinase is the prominently commercialized thrombolytic drug used for the treatment of cardiovascular diseases. The later studies on staphylokinase (SaK) showed promising results as an alternative fibrinolytic drug. The present study explores the isolation, production and purification of SaK producing Staphylococcus sp from milk samples. The potent isolate MSA4 of Staphylococcus sp. was selected for production and purification of SaK. The total activity and specific activity of purified staphylokinase was found to be 1266 IU mL-1 and 815.5 IU mg-1 respectively. The partially purified enzyme was lysed the euglobulin clot completely within 18 h of incubation and the purified enzyme showed 79% of blood clot lysis activity. PMID:25374011

Mohanasrinivasan, V; Saha, Sukanya; Nagarchi, Lubbnaz; Devi, C Subathra; Vaishnavi, B; Jemimah, S Naine

2014-11-01

316

Biodegradation of 4-chloroindole by Exiguobacterium sp. PMA.  

PubMed

Exiguobacterium sp. PMA utilized 4-chloroindole as its sole source of carbon and energy. The effect of initial concentrations of substrate on the 4-chloroindole degradation was studied and observed that strain PMA was capable of degrading 4-chloroindole up to concentration of 0.5mM. The degradation pathway of 4-chloroindole was studied for Exiguobacterium sp. PMA based on metabolites identified by gas chromatography-mass spectrometry. 4-Chloroindole was initially dehalogenated to indole that was further degraded via isatin, anthranilic acid, and salicylic acid. The potential of strain PMA to degrade 4-chloroindole in soil was monitored using soil microcosms, and it was observed that the cells of strain PMA efficiently degraded 4-chloroindole in the soil. The results of microcosm studies show that strain PMA may be used for bioremediation of 4-chloroindole-contaminated sites. This is the first report of the bacterial degradation of 4-chloroindole. PMID:25463241

Arora, Pankaj Kumar; Bae, Hanhong

2015-03-01

317

Nickel-inducible lysis system in Synechocystis sp. PCC 6803.  

PubMed

We designed and constructed a controllable inducing lysis system in Synechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (i) directly using the phage lysis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii) combining lysis genes from different phages. Significant autolysis was induced in the Synechocystis sp. PCC 6803 cells with this system by the addition of NiSO(4). Our inducible cyanobacterial lysing system eliminates the need for mechanical or chemical cell breakage and could facilitate recovery of biofuel from cyanobacteria. PMID:19995962

Liu, Xinyao; Curtiss, Roy

2009-12-22

318

Nutritional characteristics of a mixotrophic nanoflagellate, Ochromonas sp  

Microsoft Academic Search

Autotrophic and heterotrophic growth characteristics of a nano-flagellate were investigated. The flagellate,Ochromonas sp., was isolated from the northern Baltic Sea. Autotrophic growth was poor. Axenically pregrown flagellates did not increase significantly in cell number during incubation in different inorganic media. The number of flagellates remained constant 3–5 weeks in cultures kept in the light (100?mol m?2 sec?1), whereas in the

Agneta Andersson; Stefan Falk; Göran Samuelsson; Åke Hagström

1989-01-01

319

Homalomena propinqua (Araceae) is Pollinated by Parastasia sp. (Scarabaeidae)  

NSDL National Science Digital Library

Beetles are important pollinators in a lowland dipterocarp forest in Southeast Asia. Most of canopy and emergent dipterocarps are pollinated by beetles feeding on floral tissues. Some understory trees and forest floor herbs are pollinated by different types of beetles. Homalomena propinqua (Araceae) is pollinated by two specias of beetles. One of them, Parastasia sp. (Scarabaeidae), stays inside the spathe chamber and feeds on staminodes.

2004-03-09

320

Removal of Cadmium Ions from Aqueous Samples by Synechocystis sp  

Microsoft Academic Search

The biosorption of cadmium ions from aqueous solution by dried, immobilized dried and immobilized live Synechocystis sp. was investigated. Sorption of plain Ca-alginate beads, which were used as substrate for immobilization, was also studied for comparison. Removal efficiency of biosorbents was studied as a function of pH (2-8), temperature (20–40°C), initial cadmium ion concentration (50–300 mg\\/L), and contact time (0–120 min). The

?. Öztürk; B. Asl?m

2009-01-01

321

Callyspongiolide, a cytotoxic macrolide from the marine sponge Callyspongia sp.  

PubMed

A novel macrolide, callyspongiolide, whose structure was determined by comprehensive analysis of the NMR and HRMS spectra, was isolated from the marine sponge Callyspongia sp. collected in Indonesia. The compound features a carbamate-substituted 14-membered macrocyclic lactone ring with a conjugated structurally unprecedented diene-ynic side chain terminating at a brominated benzene ring. Callyspongiolide showed strong cytotoxicity against human Jurkat J16 T and Ramos B lymphocytes. PMID:24329175

Pham, Cong-Dat; Hartmann, Rudolf; Böhler, Philip; Stork, Björn; Wesselborg, Sebastian; Lin, Wenhan; Lai, Daowan; Proksch, Peter

2014-01-01

322

Communal Foraging Behavior and Recruitment Communication in Gloveria sp  

Microsoft Academic Search

The caterpillars of Gloveria sp. mark trails with a pheromone they deposit by dragging the ventral surface of the tip of the abdomen along branch pathways as they move between their communal nest and distance feeding sites. The threshold sensitivity of the caterpillar for an extract prepared from the secretory site was approximately 0.5 × 10-3 caterpillar equivalents\\/cm of trail.

T. D. Fitzgerald; D. L. A. Underwood

1998-01-01

323

Phyllosticta ophiopogonis sp. nov. from Ophiopogon japonicus (Liliaceae)  

PubMed Central

A leaf spotting disease of an ornamental variety of Ophiopogon japonicus was discovered at several locations in northern Thailand. In all cases a species of Phyllosticta was associated with the lesions. Phyllosticta ophiopogonis sp. nov. is distinguished from Phyllosticta species from Liliaceae in conidia size, mucilaginous sheath and appendage thus the species is introduced as new in this paper. The new species which causes unsightly lesions on this ornamental plant is described, illustrated and compared with other similar Phyllosticta species. PMID:23961156

Wikee, S.; Wulandari, N.F.; McKenzie, E.H.C.; Hyde, K.D.

2011-01-01

324

Natural transformation of Thermotoga sp. strain RQ7  

PubMed Central

Background Thermotoga species are organisms of enormous interest from a biotechnological as well as evolutionary point of view. Genetic modifications of Thermotoga spp. are often desired in order to fully release their multifarious potentials. Effective transformation of recombinant DNA into these bacteria constitutes a critical step of such efforts. This study aims to establish natural competency in Thermotoga spp. and to provide a convenient method to transform these organisms. Results Foreign DNA was found to be relatively stable in the supernatant of a Thermotoga culture for up to 6 hours. Adding donor DNA to T. sp. strain RQ7 at its early exponential growth phase (OD600 0.18?~?0.20) resulted in direct acquisition of the DNA by the cells. Both T. neapolitana chromosomal DNA and Thermotoga-E. coli shuttle vectors effectively transformed T. sp. strain RQ7, rendering the cells resistance to kanamycin. The kan gene carried by the shuttle vector pDH10 was detected by PCR from the plasmid extract of the transformants, and the amplicons were verified by restriction digestions. A procedure for natural transformation of Thermotoga spp. was established and optimized. With the optimized method, T. sp. strain RQ7 sustained a transformation frequency in the order of 10-7 with both genomic and plasmid DNA. Conclusions T. sp. strain RQ7 cells are naturally transformable during their early exponential phase. They acquire DNA from both closely and distantly related species. Both chromosomal DNA and plasmid DNA serve as suitable substrates for transformation. Our findings lend a convenient technical tool for the genetic engineering of Thermotoga spp. PMID:24884561

2014-01-01

325

Pestaloquinols A and B, isoprenylated epoxyquinols from Pestalotiopsis sp.  

PubMed

Two new isoprenylated epoxyquinol derivatives, pestaloquinols A (2) and B (3), and their putative biosynthetic precursor, cytosporin D (1), were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis sp. The structures of these compounds were elucidated primarily by NMR experiments. Pestaloquinols A (2) and B (3) possess a previously undescribed nonacyclic ring system and showed cytotoxicity against HeLa cells. PMID:21302965

Ding, Gang; Zhang, Fan; Chen, Hong; Guo, Liangdong; Zou, Zhongmei; Che, Yongsheng

2011-02-25

326

The complete mitochondrial genome of Scolopocryptops sp. (Chilopoda: Scolopendromorpha: Scolopocryptopidae).  

PubMed

The first complete mitogenome sequence of Scolopendromorpha is reported herein. The mitochondrial genome of Scolopocryptops sp. (Scolopendromorpha: Scolopocryptopidae) is a circular molecule of 15,119?bp in length. The orientation and gene order of the Scolopocryptops mitogenome are identical to that of Limulus polyphemus, except that the position of trnL1 and trnL2 is interchanged. TrnS1 (AGN) lacks the dihydrouridine arm. PMID:23631366

Gai, Yonghua; Ma, Huiqin; Ma, Junye; Li, Chunxiang; Yang, Qun

2014-06-01

327

Physical properties of the DNA of bacteriophage SP50  

Microsoft Academic Search

The following properties of the DNA of B. subtilis phage SP50 were established: Molecular weight (in Daltons) 102×106 (sedimentation velocity) 97×106 (viscosity) 97×106 (contour lengths of electron micrographs) Base Composition (in % GC) 41.7 (chemical analysis) 44 (melting point) 44 (buoyant density) No unusual bases were observed. The complementary strands of the DNA can be separated. The phage DNA has

N. Biswal; A. K. Kleinschmidt; H. C. Spatz; T. A. Trautner

1967-01-01

328

Two compounds from the endophytic Colletotrichum sp. of Ginkgo biloba.  

PubMed

Two compounds, apigenin-8-C-beta-D-glucopyranoside and 2-(hydroxymethylthio)ethanol, were extracted from the fermentation products of a strain of endophytic fungus, Colletotrichum sp. NTB-2, isolated from the leafstalk of Ginkgo biloba. The structures of the two compounds were determined on the basis of extensive spectroscopic analysis, including 1D and 2D NMR spectral data. The compounds wereobtained from microorganisms for the first time. PMID:21922917

Zhou, Sheng-Liang; Zhou, Song-Lin; Wang, Mei-Xia; Chen, Shuang-Lin

2011-08-01

329

Characterization of thermostable lipase from thermophilic Geobacillus sp. TW1  

Microsoft Academic Search

A novel lipase-producing thermophilic strain TW1, assigned to Geobacillus sp. TW1 based on 16S rRNA sequence, was isolated from a hot spring in China. Based on this strain, a lipase gene encoding 417 amino acids was cloned. Subsequently, the lipase gene was expressed in Escherichia coli and purified as a fusion protein with glutathione S-transferase. The results showed that the

Hebin Li; Xiaobo Zhang

2005-01-01

330

Phyllosticta ophiopogonis sp. nov. from Ophiopogon japonicus (Liliaceae).  

PubMed

A leaf spotting disease of an ornamental variety of Ophiopogon japonicus was discovered at several locations in northern Thailand. In all cases a species of Phyllosticta was associated with the lesions. Phyllosticta ophiopogonis sp. nov. is distinguished from Phyllosticta species from Liliaceae in conidia size, mucilaginous sheath and appendage thus the species is introduced as new in this paper. The new species which causes unsightly lesions on this ornamental plant is described, illustrated and compared with other similar Phyllosticta species. PMID:23961156

Wikee, S; Wulandari, N F; McKenzie, E H C; Hyde, K D

2012-01-01

331

Anagnorisma chamrani sp. n. (Lepidoptera, Noctuidae) from Iran  

PubMed Central

Abstract A new Anagnorisma species, Anagnorisma chamrani sp. n., is described from Binaloud Mountains of Khorasan-e-Razavi province in north-eastern Iran, and compared with its sister species, Anagnorisma eucratides (Boursin, 1960). The adults, and male and female genitalia of both species are illustrated in 11 figures. The genus Anagnorisma is recorded for the first time for the fauna of Iran. PMID:23950668

Gyulai, Peter; Rabieh, Mohammad Mahdi; Seraj, Ali Asghar; Ronkay, Laslo; Esfandiari, Mehdi

2013-01-01

332

SP100 Pump\\/Converter Test Loop Design  

Microsoft Academic Search

The Pump\\/ Converter Test Loop (P\\/C Loop) is the second in a series of high temperature liquid metal test loops used to validate selected Generic Flight System (GFS) components for the SP-100 Program. Key components to be tested at elevated temperatures in the P\\/C Loop include a Thermoelectric Magnetic (TEM) pump assembly, and a Thermoelectric Converter Assembly (TCA). Operation of

T. Ted Fallas; Gordon B. Kruger; Frank R. Wiltshire; Harold R. Clay; Rafael Yahalom

1994-01-01

333

SP100 space reactor power system readiness and mission flexibility  

Microsoft Academic Search

The SP-100 Space Reactor Power System (SRPS) is being developed by GE, under contract to the U.S. Department of Energy, to provide electrical power in the range of 10s to 100s of kW. The system represents an enabling technology for a wide variety of earth orbital and interplanetary science missions, nuclear electric propulsion (NEP) stages, and lunar\\/Mars surface power for

Allan T. Josloff; Donald N. Matteo; H. Sterling Bailey

1993-01-01

334

SP100 position multiplexer and analog input processor  

Microsoft Academic Search

This paper discusses the design, implementation, and performance of a working model of the Position Multiplexer-Analog Input Processor (AIP) System for the SP-100 Spacecraft. The goal of this study is to determine the practical circuit limitations, investigate the circuit\\/component degradations of the multiplexer due to radiation, develop an interference cancellation technique, and evaluate the measurement accuracy as a function of

Akbar Syed; Ray Meyer; J. Shukla; Ken Gilliland

1992-01-01

335

SP100 Fuel Pin Performance: Results from Irradiation Testing  

Microsoft Academic Search

A total of 86 experimental fuel pins with various fuel, liner, and cladding candidate materials have been irradiated in the Experimental Breeder Reactor-II (EBR-II) and the Fast Flux Test Facility (FFTF) reactor as part of the SP-100 fuel pin irradiation testing program. Postirradiation examination results from these fuel pins are key in establishing performance correlations and demonstrating the lifetime and

Bruce J. Makenas; Dean M. Paxton; Swaminathan Vaidyanathan; Martin Marietta; Carl W. Hoth

1994-01-01

336

SP100 recent achievements in thermal electric cell technology  

Microsoft Academic Search

The authors discuss achievements in enabling technology development for the conductively coupled multi-couple thermoelectric cell being designed by GE for SP-100. Results of cell ingredient testing and the correlation to pretest performance predictions are discussed. First-of-a-kind technology developments on the single-crystal high-voltage insulator and its compatibility with lithium-containing systems and strain-absorbing compliant pads are reported on. It is concluded that

D. N. Matteo; J. Bond; W. Kugler

1991-01-01

337

Fabrication of the SP100 Thermoelectric-Electromagnetic Pump  

Microsoft Academic Search

The thermoelectric electromagnetic (TEM) pump circulates lithium in the primary and secondary coolant circuits of the SP-100 Space Power Reactor System. Because of the high temperature of the coolant the pump is made primarily from niobium — 1 percent zirconium (Nb-1%Zr) alloy and is self-powered by semiconductor thermoelectric cells. Design features needed to optimize pumping efficiency such as bonded dissimilar

Robert E. Butler; Carlos Martinez; Thomas J. Ruffo; Edwin D. Sayre

1994-01-01

338

Reactor design of the SP100 Nuclear Assembly Test  

Microsoft Academic Search

The Nuclear Assembly Test is currently being designed to demonstrate the performance characteristics of a 100-kWe version of the power source for the SP-100 Generic Flight System. Particular emphasis will be placed upon the operation of the prototypical ground test reactor under conditions of high-working temperatures and long life. The key features of the reactor include a small, compact core

Charles L. Cowan; Alan Chung; Samuel Kaplan; Thomas F. Marcille; Robert Protsik; Samuel S. Stewart

1991-01-01

339

Reactor design of the SP100 nuclear assembly test  

Microsoft Academic Search

The Nuclear Assembly Test is currently being designed to demonstrate the performance characteristics of a 100-kWe version of the power source for the SP-100 Generic Flight System. Particular emphasis will be placed upon the operation of the prototypical ground test reactor under conditions of high-working temperatures and long life. The key features of the reactor include a small, compact core

Charles L. Cowan; Alan Chung; Samuel Kaplan; Thomas F. Marcille; Robert Protsik; Samuel S. Stewart

1991-01-01

340

Safety provisions for the SP100 Nuclear Assembly Test article  

Microsoft Academic Search

The SP-100 Nuclear Assembly Test (NAT) article is charged with the validation of such a fast-spectrum, liquid metal-cooled reactor's integrated reactor, control, and shield performance characteristics in a spacelike environment. The NAT facility is designed for safe assembly, operation, disassembly, and disposal. Attention is presently given to the selection and classification of postulated accidents, safety design criteria, reactor trip parameters,

Mark I. Temme; Dennis R. Damon; Norman E. Hackford; Chuong T. Ha; Robert L. Mapes; David D. Miller; Michael A. Smith

1991-01-01

341

PWC11 fuel pin development for SP100  

Microsoft Academic Search

GE has developed a high temperature fuel pin for the SP-100 Space Reactor. The Nb-1% Zr alloy PWC-11 was selected over the conventional Nb-1% Ar alloy for the fuel pin cladding because the higher creep strength enables a significant reduction in system mass. PWC-11 has 0.1% carbon to provide the higher creep and tensile strength at the operating temperature. PWC-11

Edwin D. Sayre; Robert E. Butler; Mike Kangilaski

1992-01-01

342

PWC11 fuel pin development for SP100  

Microsoft Academic Search

GE has developed a high temperature fuel pin for the SP-100 Space Reactor. The Nb-1% Zr alloy PWC-11 was selected over the conventional Nb-1% Ar alloy for the fuel pin cladding because the higher creep strength enables a significant reduction in system mass. PWC-11 has 0.1% carbon to provide the higher creep and tensile strength at the operating temperature. PWC-11

Edwin D. Sayre; Robert E. Butler; Mike Kangilaski

1992-01-01

343

Larinus berti sp. n. (Coleoptera, Curculionidae, Lixinae) from North Africa  

PubMed Central

Abstract A new species, Larinus berti sp. n. is described from Morocco and assigned to subgenus Cryphopus Petri, 1907 (Curculionidae: Lixinae; Lixini). Diagnostic characters of the new species are large size, elongate-ovate body, bisulcate sub-quadrangular rostrum, triangularly raised dorsum of rostrum, flat subgena and submentum, Y-shaped apodeme of sternite VIII of female and thin nodulus of spermatheca. PMID:24194653

Gültekin, Levent; Alonso-Zarazaga, Miguel A.

2013-01-01

344

SP Betting as a Self-Enforcing Implicit Cartel  

Microsoft Academic Search

A large share of the UK off-course horse racing betting market involves winning payouts determined at Starting Prices (SP). This implies that gamblers can bet with off-course bookies on any horse before a race at the final pre-race odds as set by on-course bookies for that horse. Given the oligopolistic structure of the off-course gambling market in the UK, a

Adi Schnytzer; Avichai Snir

2011-01-01

345

SP Betting as a Self-Enforcing Implicit Cartel  

Microsoft Academic Search

A large share of the UK off-course horse racing betting market involves winning payouts determined at Starting Prices (SP). This implies that gamblers can bet with off-course bookies on any horse before a race at the final pre-race odds as set by on-course bookies for that horse. Given the oligopolistic structure of the off-course gambling market in the UK, a

Adi Schnytzer; Avichai Snir

2008-01-01

346

New Oxidized Zoanthamines from a Canary Islands Zoanthus sp.  

PubMed Central

Three new norzoanthamine-type alkaloids, named 2-hydroxy-11-ketonorzoanthamide B (1), norzoanthamide B (2) and 15-hydroxynorzoanthamine (3), were isolated from Zoanthus sp. specimens collected at the Canary Islands. Their structures were determined by interpretation of NMR and HR-ESIMS data. Relative configurations of their chiral centers were proposed on the basis of ROESY spectra and by comparison of their spectroscopic data with those of the well-known compound, norzoanthamine. PMID:25317536

Cen-Pacheco, Francisco; Norte Martín, Manuel; Fernández, José Javier; Hernández Daranas, Antonio

2014-01-01

347

Feeding by heterotrophic dinoflagellates and ciliates on the free-living dinoflagellate Symbiodinium sp. (Clade E).  

PubMed

To investigate heterotrophic protists grazing on Symbiodinium sp., we tested whether the common heterotrophic dinoflagellates Gyrodinium dominans, Gyrodinium moestrupii, Gyrodinium spirale, Oblea rotundata, Oxyrrhis marina, and Polykrikos kofoidii and the ciliates Balanion sp. and Parastrombidinopsis sp. preyed on the free-living dinoflagellate Symbiodinium sp. (clade E). We measured the growth and ingestion rates of O. marina and G. dominans on Symbiodinium sp. as a function of prey concentration. Furthermore, we compared the results to those obtained for other algal prey species. In addition, we measured the growth and ingestion rates of other predators at single prey concentrations at which these rates of O. marina and G. dominans were saturated. All predators tested in the present study, except Balanion sp., preyed on Symbiodinium sp. The specific growth rates of O. marina and G. dominans on Symbiodinium sp. increased rapidly with increasing mean prey concentration < ca. 740-815 ng C/ml (7,400-8,150 cells/ml), but became saturated at higher concentrations. The maximum growth rates of O. marina and G. dominans on Symbiodinium sp. (0.87 and 0.61/d) were much higher than those of G. moestrupii and P. kofoidii (0.11 and 0.04/d). Symbiodinium sp. did not support positive growth of G. spirale, O. rotundata, and Parastrombidinopsis sp. However, the maximum ingestion rates of P. kofoidii and Parastrombidinopsis sp. (6.7-10.0 ng C/predator/d) were much higher than those of O. marina and G. dominans on Symbiodinium sp. (1.9-2.1 ng C/predator/d). The results of the present study suggest that Symbiodinium sp. may increase or maintain the populations of some predators. PMID:24102740

Jeong, Hae Jin; Lim, An Suk; Yoo, Yeong Du; Lee, Moo Joon; Lee, Kyung Ha; Jang, Tae Young; Lee, Kitack

2014-01-01

348

SP-100 multimegawatt scaleup to meet electric propulsion mission requirements  

NASA Astrophysics Data System (ADS)

The SP-100 nuclear heat source technology, utilizing uranium nitride fuel clad in PWC-11 in a fast reactor with lithium coolant circulated by an electromagnetic pump, is shown to be directly extrapolatable to thermal power levels that meet NASA nuclear electric propulsion requirements using different power conversion techniques. The SP-100 nuclear technology can be applied to missions with NEP (nuclear electric propulsion) requirements as low as tens of kWe to tens of MWe. It is pointed out that the SP-100 heat source has a great advantage of very long lifetime capability, since it utilizes very rugged refractory metal fuel pins and is independent of the power conversion scheme chosen for a given mission. The only moving parts in the nuclear subsystems are the control rods moved to compensate for fuel enrichment degradation due to fission and for power shutdown. Lowest alpha values in the range of interest for potential NASA missions are predicted for the dynamic Rankine and static HYTEC conversion systems.

Newkirk, D. W.; Salamah, S. A.; Stewart, S. L.; Pluta, P. R.

349

Users guide to the Argonne SP scheduling system  

SciTech Connect

During the past five years scientists discovered that modern UNIX workstations connected with ethernet and fiber networks could provide enough computational performance to compete with the supercomputers of the day. As this concept became increasingly popular, the need for distributed queuing and scheduling systems became apparent. Today, supercomputers, such as Argonne National Laboratory`s IBM SP system, can provide more CPU and networking speed than can be obtained from these networks of workstations. These modern supercomputers look like clusters of workstations, however, so developers felt that the scheduling systems that were previously used on clusters of workstations should still apply. After trying to apply some of these scheduling systems to Argonne`s SP environment, it became obvious that these two computer environments have very different scheduling needs. Recognizing this need and realizing that no one has addressed it, we developed a new scheduling system. The approach taken in creating this system was unique in that user input and interaction were encouraged throughout the development process. Thus, a scheduler was built that actually worked the way the users wanted it to work. This document serves a dual purpose. It is both a user`s guide and an administrator`s guide for the ANL SP scheduling system. Look for revisions to this guide that will be appearing.

Lifka, D.A. [Argonne National Lab., IL (United States)]|[Illinois Inst. of Technology, IL (United States); Henderson, M.W.; Rayl, K. [Argonne National Lab., IL (United States)

1995-05-01

350

Alkaline protease from Thermoactinomyces sp. RS1 mitigates industrial pollution.  

PubMed

Proteases have found a wide application in the several industrial processes, such as laundry detergents, protein recovery or solubilization, prion degradation, meat tenderizations, and in bating of hides and skins in leather industries. But the main hurdle in industrial application of proteases is their economical production on a large scale. The present investigation aimed to exploit the locally available inexpensive agricultural and household wastes for alkaline protease production using Thermoactinomyces sp. RS1 via solid-state fermentation (SSF) technique. The alkaline enzyme is potentially useful as an additive in commercial detergents to mitigate pollution load due to extensive use of caustic soda-based detergents. Thermoactinomyces sp. RS1 showed good protease production under SSF conditions of 55 °C, pH 9, and 50 % moisture content with potato peels as solid substrate. The presented findings revealed that crude alkaline protease produced by Thermoactinomyces sp. RS1 via SSF is of potential application in silver recovery from used X-ray films. PMID:24122212

Verma, Amit; Ansari, Mohammad W; Anwar, Mohmmad S; Agrawal, Ruchi; Agrawal, Sanjeev

2014-05-01

351

Network Analysis of Plasmidomes: The Azospirillum brasilense Sp245 Case  

PubMed Central

Azospirillum brasilense is a nitrogen-fixing bacterium living in association with plant roots. The genome of the strain Sp245, isolated in Brazil from wheat roots, consists of one chromosome and six plasmids. In this work, the A. brasilense Sp245 plasmids were analyzed in order to shed some light on the evolutionary pathways they followed over time. To this purpose, a similarity network approach was applied in order to identify the evolutionary relationships among all the A. brasilense plasmids encoded proteins; in this context a computational pipeline specifically devoted to the analysis and the visualization of the network-like evolutionary relationships among different plasmids molecules was developed. This information was supplemented with a detailed (in silico) functional characterization of both the connected (i.e., sharing homology with other sequences in the dataset) and the unconnected (i.e., not sharing homology) components of the network. Furthermore, the most likely source organism for each of the genes encoded by A. brasilense plasmids was checked, allowing the identification of possible trends of gene loss/gain in this microorganism. Data obtained provided a detailed description of the evolutionary landscape of the plasmids of A. brasilense Sp245, suggesting some of the molecular mechanisms responsible for the present-day structure of these molecules. PMID:25610702

Fondi, Marco

2014-01-01

352

Network Analysis of Plasmidomes: The Azospirillum brasilense Sp245 Case.  

PubMed

Azospirillum brasilense is a nitrogen-fixing bacterium living in association with plant roots. The genome of the strain Sp245, isolated in Brazil from wheat roots, consists of one chromosome and six plasmids. In this work, the A. brasilense Sp245 plasmids were analyzed in order to shed some light on the evolutionary pathways they followed over time. To this purpose, a similarity network approach was applied in order to identify the evolutionary relationships among all the A. brasilense plasmids encoded proteins; in this context a computational pipeline specifically devoted to the analysis and the visualization of the network-like evolutionary relationships among different plasmids molecules was developed. This information was supplemented with a detailed (in silico) functional characterization of both the connected (i.e., sharing homology with other sequences in the dataset) and the unconnected (i.e., not sharing homology) components of the network. Furthermore, the most likely source organism for each of the genes encoded by A. brasilense plasmids was checked, allowing the identification of possible trends of gene loss/gain in this microorganism. Data obtained provided a detailed description of the evolutionary landscape of the plasmids of A. brasilense Sp245, suggesting some of the molecular mechanisms responsible for the present-day structure of these molecules. PMID:25610702

Orlandini, Valerio; Emiliani, Giovanni; Fondi, Marco; Maida, Isabel; Perrin, Elena; Fani, Renato

2014-01-01

353

Komagataella populi sp. nov. and Komagataella ulmi sp. nov., two new methanol assimilating yeasts from exudates of deciduous trees.  

PubMed

Two new species of the methanol assimilating ascosporic yeast genus Komagataella are described. Komagataella populi sp. nov. (NRRL YB-455, CBS 12362, type strain, MycoBank accession number = 564110) was isolated from an exudate on a cottonwood tree (Populus deltoides), Peoria, Illinois, USA, and Komagataella ulmi sp. nov. (NRRL YB-407, CBS 12361, type strain, MycoBank accession number = 564111) was isolated from the exudate on an elm tree (Ulmus americana), also growing in Peoria, Illinois. The species were resolved from divergence in gene sequences for domains D1/D2 LSU rRNA, ITS1-5.8S-ITS2, mitochondrial small subunit rRNA, RNA polymerase subunit 1 and translation elongation factor-1?. Species of Komagataella assimilate few carbon compounds and are unlikely to be resolved from differences in standard growth and fermentation tests. For this reason, separation of species is dependent on gene sequence analysis. PMID:22302468

Kurtzman, Cletus P

2012-02-01

354

Gyrodactylus poeciliae n. sp. and G. milleri n. sp. (Monogenea: Gyrodactylidae) from Poecilia caucana (Steindachner) in Venezuela.  

PubMed

Two new species of Gyrodactylus are described from the South American live-bearing fish Poecilia caucana from Venezuela. Gyrodactylus poeciliae n. sp. is characterised by conspicuous ventral bar processes and by marginal hooks in which the tips are barely curved. Morphologically, the species resembles G. bullatarudis and G. costaricensis, and its relationship to the former was confirmed by sequencing the Internal Transcribed Spacers (ITS-1 and ITS-2) and the 5.8S ribosomal gene of the ribosomal DNA. Gyrodactylus milleri n. sp. was characterised by large, sharply angled marginal hook sickles in which the tips overhang the toes. This species resembled most closely G. turnbulli from guppies. These descriptions confirm the occurrence of G. turnbulli and G. bullatarudis-like gyrodactylids on a range of South American poeciliid fishes. PMID:10966215

Harris, P D; Cable, J

2000-10-01

355

Description of Thermococcus kodakaraensis sp. nov., a well studied hyperthermophilic archaeon previously reported as Pyrococcus sp. KOD1  

PubMed Central

A hyperthermophilic archaeal strain, KOD1, isolated from a solfatara on Kodakara Island, Japan, has previously been reported as Pyrococcus sp. KOD1. However, a detailed phylogenetic tree, made possible by the recent accumulation of 16S rRNA sequences of various species in the order Thermococcales, indicated that strain KOD1 is a member of the genus Thermococcus. We performed DNA–DNA hybridization tests against species that displayed high similarity in terms of 16S ribosomal DNA sequences, including Thermococcus peptonophilus and Thermococcus stetteri. Hybridization results and differences in growth characteristics and substrate utilization differentiated strain KOD1 from T. peptonophilus and T. stetteri at the species level. Our results indicate that strain KOD1 represents a new species of Thermococcus, which we designate as Thermococcus kodakaraensis KOD1 sp. nov. PMID:15810436

Atomi, Haruyuki; Fukui, Toshiaki; Kanai, Tamotsu; Morikawa, Masaaki; Imanaka, Tadayuki

2004-01-01

356

Lactobacillus farraginis sp. nov. and Lactobacillus parafarraginis sp. nov., heterofermentative lactobacilli isolated from a compost of distilled shochu residue.  

PubMed

Five strains of lactic acid bacteria were isolated from a compost of distilled shochu residue in Japan. The isolates were separated into two groups on the basis of 16S rRNA gene sequence similarity, and two subclusters were formed that comprised micro-organisms closely related to Lactobacillus buchneri, L. diolivorans, L. hilgardii, L. kefiri, L. parabuchneri and L. parakefiri. DNA-DNA relatedness results revealed that the isolates could be separated into two groups, and these groups correlated well with the subclusters generated using the phylogenetic analysis. Moreover, the levels of DNA-DNA relatedness showed clear separation of the two groups from their phylogenetic relatives. Therefore, the two groups represent two novel species, for which the names Lactobacillus farraginis sp. nov. (type strain NRIC 0676(T)=JCM 14108(T)=DSM 18382(T)) and Lactobacillus parafarraginis sp. nov. (type strain NRIC 0677(T)=JCM 14109(T)=DSM 18390(T)) are proposed. PMID:17392191

Endo, Akihito; Okada, Sanae

2007-04-01

357

Paramaritremopsis solielangi n. sp. and Microphallus kinsellai n. sp. (Digenea: Microphallidae) parasites of Charadrii (Aves) of Belize (Central America).  

PubMed

The authors describe and illustrate two trematodes from Belize (Central America): Paramaritremopsis solielangi n. sp. from the small intestine of Arenaria interpres is characterised by a body length of 478 microm, two short and pre-acetabular caeca, part of the uterus in close association with the cirrus-sac and left caecum, vitelline glands in the shape of a horseshoe, a short pre-ovarian cirrus-sac containing a long, cylindrical, voluminous and unarmed cirrus (size when evaginated: 150 x 20-30 microm) and Microphallus kinsellai n. sp. from the caeca of Actitis macularia characterised by a body length of 370 microm and a phallus which is 30 microm in diameter and asymmetrical (basically a pad with a moderately developed accessory lobe) and a straight ejaculatory canal. Levinseniella carteretensis is another microphallid recovered from Arenaria interpres. The term of "phallus" is proposed to name the male copulatory organ which characterizes the Microphallinae. PMID:10966220

Canaris, A G; Deblock, S

2000-10-01

358

Sarcocystis dubeyella n. sp. and Sarcocystis phacochoeri n. sp. (Protozoa: Sarcocystidae) from the warthog (Phacochoerus aethiopicus) in South Africa.  

PubMed

Sarcocystis dubeyella n. sp. and S. phacochoeri n. sp. from muscle fibers of the skeletal musculature of two warthogs in South Africa are described by light and and electron microscopy. Sarcocystis dubeyella sarcocysts are macroscopic (up to 12 mm long and 1 mm wide), with a parasite-induced encapsulation of the host muscle fiber in which the plasma membrane of the latter remained unaltered. The sarcocyst wall is characterized by evenly arranged, irregularly semicircular or rectangular villar protrusions (5.0 x 2.8-11.0 microns) with indented margins and no specific content. Sarcocystis phacochoeri formed filiform microcysts (up to 4 mm long and 0.13 mm wide). Its cyst wall is provided with tightly packed, molarlike villar protrusions (1.6-3.3 x 1.7-3.3 microns), with smooth margins, hollow on one side, and with longitudinal condensations of the fine granular matrix at various locations in the interior. PMID:9495038

Stolte, M; Odening, K; Quandt, S; Bengis, R G; Bockhardt, I

1998-01-01

359

Funaria maryanneae n. sp. and Proleptonchus weischeri n. sp. (Nematoda: Dorylaimida) from Europe and New Synonyms in Leptonchoidea  

PubMed Central

Funaria maryanneae n. sp., distinguished by its large size and long prerectum, is described from specimens collected in Bad Sooden, Germany, D. B. R. This nematode was collected also from Fräkmüntegg (Mt. Pilatus), Switzerland. Proleptonchus weischeri n. sp., with short hemispheroid female tail and relatively anterior vulva, is described from specimens collected in Kaufunger Wald near Kassel, Germany, D. B. R. This is the first report of either genus from the continent of Europe. Leptonchus parisii Zullini, 1973, from Chiapas, Mexico, becomes a junior synonym of Funaria obtusa (Thorne, 1939) Goseco, Ferris and Ferris, 1974. Leptonchus acutus Zulliui, 1973 becomes Funaria acuta n. comb., and Funaria rothi Goseco, Ferris and Ferris, 1974 becomes a junior synonym of Funaria acuta. PMID:19308225

Goseco, C. G.; Ferris, V. R.

1976-01-01

360

Two novel ascomycetous yeast species, Wickerhamomyces scolytoplatypi sp. nov. and Cyberlindnera xylebori sp. nov., isolated from ambrosia beetle galleries.  

PubMed

Thirteen strains of yeasts were isolated from ambrosia beetle galleries at several sites in Japan. Based on the morphological and biochemical characteristics and phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene of the yeasts, 10 strains were shown to represent a novel species of the genus Wickerhamomyces, described as Wickerhamomyces scolytoplatypi sp. nov. (type strain NBRC 11029(T) = CBS 12186(T)), and were closely related to Wickerhamomyces hampshirensis. The three other strains represented a novel species of the genus Cyberlindnera, described as Cyberlindnera xylebori sp. nov. (type strain NBRC 11048(T) = CBS 12187(T)), and were closely related to Cyberlindnera euphorbiiphila. It is suggested that these species are associated with ambrosia beetles and we consider ambrosia beetle galleries as good sources of novel yeasts. PMID:23667144

Ninomiya, Shinya; Mikata, Kozaburo; Kajimura, Hisashi; Kawasaki, Hiroko

2013-07-01

361

Chemical reactivity of Synechococcus sp. PCC 7002 and Synechocystis sp. PCC 6803 hemoglobins: covalent heme attachment and bishistidine coordination  

PubMed Central

In the absence of an exogenous ligand, the hemoglobins from the cyanobacteria Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002 coordinate the heme group with two axial histidines (His46 and His70). These globins also form a covalent linkage between the heme 2-vinyl substituent and His117. The in vitro mechanism of heme attachment to His117 was examined with a combination of site-directed mutagenesis, NMR spectroscopy, and optical spectroscopy. The results supported an electrophilic addition with vinyl protonation being the rate-determining step. Replacement of His117 with a cysteine demonstrated that the reaction could occur with an alternative nucleophile. His46 (distal histidine) was implicated in the specificity of the reaction for the 2-vinyl group as well as protection of the protein from oxidative damage caused by exposure to exogenous H2O2. PMID:21240532

Nothnagel, Henry J.; Preimesberger, Matthew R.; Pond, Matthew P.; Winer, Benjamin Y.; Adney, Emily M.

2011-01-01

362

Integrated Bacillus sp. immobilized cell reactor and Synechocystis sp. algal reactor for the treatment of tannery wastewater.  

PubMed

The wastewater discharged from leather industries lack biodegradability due to the presence of xenobiotic compounds. The primary clarification and aerobic treatment in Bacillus sp. immobilized Chemo Autotrophic Activated Carbon Oxidation (CAACO) reactor removed considerable amount of pollution parameters. The residual untreated organics in the wastewater was further treated in algal batch reactor inoculated with Synechocystis sp. Sodium nitrate, K(2)HPO(4), MgSO(4).7H(2)O, NH(4)Cl, CaCl(2)·2H(2)O, FeCl(3) (anhydrous), and thiamine hydrochloride, rice husk based activated carbon (RHAC), immobilization of Bacillus sp. in mesoporous activated carbon, sand filter of dimensions diameter, 6 cm and height, 30 cm; and the CAACO reactor of dimensions diameter, 5.5 cm and height, 30 cm with total volume 720 ml, and working volume of 356 ml. In the present investigation, the CAACO treated tannery wastewater was applied to Synechocystis sp. inoculated algal batch reactor of hydraulic residence time 24 h. The BOD(5), COD, and TOC of treated wastewater from algal batch reactor were 20 ± 7, 167 ± 29, and 78 ± 16 mg/l respectively. The integrated CAACO system and Algal batch reactor was operated for 30 days and they accomplished a cumulative removal of BOD(5),COD, TOC, VFA and sulphide as 98 %, 95 %, 93 %, 86 %, and 100 %, respectively. The biokinetic constants for the growth of algae in the batch reactor were specific growth rate, 0.095(day(-1)) and yield coefficient, 3.15 mg of algal biomass/mg of COD destructed. The degradation of xenobiotic compounds in the algal batch reactor was confirmed through HPLC and FT-IR techniques. The integrated CAACO-Algal reactor system established a credible reduction in pollution parameters in the tannery wastewater. The removal mechanism is mainly due to co-metabolism between algae and bacterial species and the organics were completely metabolized rather than by adsorption. PMID:22528997

Sekaran, G; Karthikeyan, S; Nagalakshmi, C; Mandal, A B

2013-01-01

363

Thermococcus paralvinellae sp. nov. and Thermococcus cleftensis sp. nov. of hyperthermophilic heterotrophs from deep-sea hydrothermal vents.  

PubMed

Two heterotrophic hyperthermophilic strains, ES1(T) and CL1(T), were isolated from Paralvinella sp. polychaete worms collected from active hydrothermal vent chimneys in the north-eastern Pacific Ocean. Both were obligately anaerobic and produced H2S in the presence of elemental sulfur and H2. Complete genome sequences are available for both strains. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strains are more than 97% similar to most other species of the genus Thermococcus. Therefore, overall genome relatedness index analyses were performed to establish that these strains are novel species. For each analysis, strain ES1(T) was determined to be most similar to Thermococcus barophilus MP(T), while strain CL1(T) was determined to be most similar to Thermococcus sp. 4557. The average nucleotide identity scores for these strains were 84% for strain ES1(T) and 81% for strain CL1(T), genome-to-genome direct comparison scores were 23% for strain ES1(T) and 47% for strain CL1(T), and the species identification scores were 89% for strain ES1(T) and 88% for strain CL1(T). For each analysis, strains ES1(T) and CL1(T) were below the species delineation cut-off. Therefore, based on their whole genome sequences, strains ES1(T) and CL1(T) are suggested to represent novel species of the genus Thermococcus for which the names Thermococcus paralvinellae sp. nov. and Thermococcus cleftensis sp. nov. are proposed, respectively. The type strains are ES1(T) (?=DSM 27261(T)?=KACC 17923(T)) and CL1(T) (?=DSM 27260(T)?=KACC 17922(T)). PMID:25082851

Hensley, Sarah A; Jung, Jong-Hyun; Park, Cheon-Seok; Holden, James F

2014-11-01

364

The family Carabodidae (Acari, Oribatida) VIII. The genus Machadocepheus (first part) Machadocepheus leoneae sp. n. and Machadocepheus rachii sp. n. from Gabon  

PubMed Central

Abstract The genus Machadocepheus, being one of the more complex genera of the Carabodidae family, is briefly outlined to demonstrate this complexity. Descriptions of two new species from Gabon, Machadocepheus leoneae sp. n. and Machadocepheus rachii sp. n. are given. PMID:25561826

Fernandez, Nestor; Theron, Pieter; Rollard, Christine; Leiva, Sergio

2014-01-01

365

Mild ArI-catalyzed C(sp²)-H or C(sp³)-H functionalization/C-O formation: an intriguing catalyst-controlled selectivity switch.  

PubMed

A tandem C(sp(2))-H and C(sp(3))-H functionalization/C-O bond formation catalyzed by iodine(III) reagents generated in situ has been developed. The method shows wide scope under mild conditions and exhibits an unprecedented selectivity profile that can be switched depending on the catalyst employed. PMID:25156610

Wang, Xueqiang; Gallardo-Donaire, Joan; Martin, Ruben

2014-10-01

366

Gap junctional communication modulates gene transcription by altering the recruitment of Sp1 and Sp3 to connexin-response elements in osteoblast promoters  

NASA Technical Reports Server (NTRS)

Loss-of-function mutations of gap junction proteins, connexins, represent a mechanism of disease in a variety of tissues. We have shown that recessive (gene deletion) or dominant (connexin45 overexpression) disruption of connexin43 function results in osteoblast dysfunction and abnormal expression of osteoblast genes, including down-regulation of osteocalcin transcription. To elucidate the molecular mechanisms of gap junction-sensitive transcriptional regulation, we systematically analyzed the rat osteocalcin promoter for sensitivity to gap junctional intercellular communication. We identified an Sp1/Sp3 containing complex that assembles on a minimal element in the -70 to -57 region of the osteocalcin promoter in a gap junction-dependent manner. This CT-rich connexin-response element is necessary and sufficient to confer gap junction sensitivity to the osteocalcin proximal promoter. Repression of osteocalcin transcription occurs as a result of displacement of the stimulatory Sp1 by the inhibitory Sp3 on the promoter when gap junctional communication is perturbed. Modulation of Sp1/Sp3 recruitment also occurs on the collagen Ialpha1 promoter and translates into gap junction-sensitive transcriptional control of collagen Ialpha1 gene expression. Thus, regulation of Sp1/Sp3 recruitment to the promoter may represent a potential general mechanism for transcriptional control of target genes by signals passing through gap junctions.

Stains, Joseph P.; Lecanda, Fernando; Screen, Joanne; Towler, Dwight A.; Civitelli, Roberto

2003-01-01

367

Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163  

PubMed Central

Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by 1H NMR. Ten known compounds, including angucycline, diketopiperazine and ?-carboline derivatives 1–10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes. PMID:24857962

Dashti, Yousef; Grkovic, Tanja; Abdelmohsen, Usama Ramadan; Hentschel, Ute; Quinn, Ronald J.

2014-01-01

368

Virulence, frequency, and distribution of races of Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei identified in the United States in 2008 and 2009  

Technology Transfer Automated Retrieval System (TEKTRAN)

Puccinia striiformis f. sp. tritici (PST) and P. striiformis f. sp. hordei (PSH), the causal agents of stripe rust on wheat and barley, respectively, can change rapidly in virulence, which may overcome resistance in cultivars and result in severe epidemics. To monitor virulence changes in the patho...

369

Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction  

PubMed Central

Prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases and hemicellulases that could be useful in second-generation bioethanol production. In this work, cellulolytic bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. There was a predominance of Gram positive isolates that belonged to the phyla Proteobacteria and Firmicutes. Many primary isolates with cellulolytic activity were not pure cultures. From these consortia, isolation of pure constituents was attempted in order to test the hypothesis whether microbial consortia are needed for full degradation of complex substrates. Two isolates, CB1-2-A-5 and VG-4-A-2, were obtained as the pure constituents of CB1-2 and VG-4 consortia, respectively. Based on 16S RNA sequence, they could be classified as Variovorax paradoxus and Paenibacillus alvei. Noteworthy, only VG-4 consortium showed measurable xylan degrading capacity and signs of filter paper degradation. However, no xylan or filter paper degrading capacities were observed for the pure cultures isolated from it, suggesting that other members of this consortium were necessary for these hydrolyzing activities. Our results indicated that Paenibacillus sp. and Variovorax sp. as well as VG-4 consortium, might be a useful source of hydrolytic enzymes. Moreover, although Variovorax sp. had been previously identified in metagenomic studies of cellulolytic communities, this is the first report on the isolation and characterization of this microorganism as a cellulolytic genus. PMID:23301200

Ghio, Silvina; Lorenzo, Gonzalo Sabarís Di; Lia, Verónica; Talia, Paola; Cataldi, Angel; Grasso, Daniel; Campos, Eleonora

2012-01-01

370

Cigarette Smoke Induces MUC5AC Protein Expression through the Activation of Sp1*  

PubMed Central

Cigarette smoke (CS) exposure is associated with increased mucus production and chronic obstructive pulmonary disease (COPD). MUC5AC is the major inducible mucus gene in the airway. The purpose of this investigation was to elucidate the mechanisms of CS-induced activation of MUC5AC gene transcription. We observed that the region ?3724/?3224 of the MUC5AC promoter is critical for CS-induced gene transcriptional activity and that this region contains two Sp1 binding sites. Using a lung-relevant model, we observed that CS increased nuclear Sp1 protein expression. Consequently, CS exposure resulted in enhanced Sp1-DNA binding activity and Sp1 trans-activation. Co-transfection of the MUC5AC-luc reporter with Sp1 expression plasmids resulted in significantly increased MUC5AC-luc activity, whereas co-treatment with mithramycin A, a Sp1 inhibitor, abolished CS-induced MUC5AC promoter activity. Using mobility shift assay and chromatin immunoprecipitation, we demonstrated that two Sp1 binding sites in the MUC5AC promoter are functional and responsive to CS exposure. A mutation of either Sp1 binding site in the MUC5AC promoter significantly decreased CS-induced promoter activity. Together, these data indicate that CS induces MUC5AC gene transcription predominantly through increased Sp1 nuclear protein levels and increased Sp1 binding to its promoter region. PMID:22700966

Di, Y. Peter; Zhao, Jinming; Harper, Richart

2012-01-01

371

An inhibitor domain in Sp3 regulates its glutamine-rich activation domains.  

PubMed Central

Sp3 is a ubiquitously expressed human transcription factor closely related to Sp1 and Sp4. All three proteins contain a highly conserved DNA binding domain and two glutamine-rich regions, suggesting that they possess similar activation functions. In our previous experiments, however, Sp3 failed to activate transcription. Instead, it repressed Sp1-mediated transcriptional activation, suggesting that it is an inhibitory member of this family of regulatory factors. Here we show that Sp3 can also act as a positive regulator of transcription. The glutamine-rich domains on their own have a strong activation function and interact with the TATA box binding protein (TBP)-associated factor dTAFII110. However, in full-length Sp3 as well as in Gal4-Sp3 fusion proteins, both activation domains are silenced by an inhibitory domain located between the second glutamine-rich region and the DNA binding domain. The inhibitory domain completely suppressed transcriptional activation when fused to a heterologous glutamine-rich domain but only moderately suppressed transcription when linked to an acidic activation domain. Site-directed mutagenesis identified a stretch of highly charged amino acid residues essential for inhibitor function. Substitution of the amino acid triplet KEE by alanine residues within this region changed the almost transcriptionally inactive Sp3 into a strong activator. Our results suggest that the transcriptional activity of Sp3 might be regulated in vivo by relief of inhibition. Images PMID:8896459

Dennig, J; Beato, M; Suske, G

1996-01-01

372

Purification of native surfactant protein SP-A from pooled amniotic fluid and bronchoalveolar lavage.  

PubMed

Surfactant protein SP-A is a hydrophilic glycoprotein, similar to SP-D, which plays an important role in pulmonary surfactant homeostasis and innate immunity. SP-A is actively expressed in the alveolar type II cells and Clara cells. Their basic structure consists of triple-helical collagen region and a C-terminal carbohydrate recognition domain (CRD). By binding to the infectious microbes, SP-A (like SP-D) are involved in pathogen opsonization and agglutination and subsequent clearance of the microorganism, via recruitment of phagocytic cells via receptors for the collagen region. SP-A has also been localized at extra-pulmonary sites such as salivary epithelium, amniotic fluid, prostate glands, and semen. The presence of SP-A in fetal and maternal tissue and amniotic fluid suggests it is involved in pregnancy and labor. Native SP-A can be purified from amniotic fluid and bronchiolar lavage fluid (BALF) via affinity chromatography. In addition, we also report here a procedure to express and purify a recombinant form of trimeric CRD in Escherichia coli. The availability of highly pure native SP-A and CRD region can be central to studies that examine the diverse roles that SP-A play in surfactant homeostasis, pulmonary infection and inflammation and pregnancy. PMID:24218266

Karbani, Najmunisa; Dodagatta-Marri, Eswari; Qaseem, Asif S; Madhukaran, Priyaa; Waters, Patrick; Tsolaki, Anthony G; Madan, Taruna; Kishore, Uday

2014-01-01

373

Transcriptional Activation of REST by Sp1 in Huntington's Disease Models  

PubMed Central

In Huntington's disease (HD), mutant huntingtin (mHtt) disrupts the normal transcriptional program of disease neurons by altering the function of several gene expression regulators such as Sp1. REST (Repressor Element-1 Silencing Transcription Factor), a key regulator of neuronal differentiation, is also aberrantly activated in HD by a mechanism that remains unclear. Here, we show that the level of REST mRNA is increased in HD mice and in NG108 cells differentiated into neuronal-like cells and expressing a toxic mHtt fragment. Using luciferase reporter gene assay, we delimited the REST promoter regions essential for mHtt-mediated REST upregulation and found that they contain Sp factor binding sites. We provide evidence that Sp1 and Sp3 bind REST promoter and interplay to fine-tune REST transcription. In undifferentiated NG108 cells, Sp1 and Sp3 have antagonistic effect, Sp1 acting as an activator and Sp3 as a repressor. Upon neuronal differentiation, we show that the amount and ratio of Sp1/Sp3 proteins decline, as does REST expression, and that the transcriptional role of Sp3 shifts toward a weak activator. Therefore, our results provide new molecular information to the transcriptional regulation of REST during neuronal differentiation. Importantly, specific knockdown of Sp1 abolishes REST upregulation in NG108 neuronal-like cells expressing mHtt. Our data together with earlier reports suggest that mHtt triggers a pathogenic cascade involving Sp1 activation, which leads to REST upregulation and repression of neuronal genes. PMID:21179468

Ravache, Myriam; Weber, Chantal; Mérienne, Karine; Trottier, Yvon

2010-01-01

374

Screening and characterization of bioflocculant produced by isolated Klebsiella sp.  

PubMed

Sixteen strains of polymer-producing bacteria were isolated from the activated sludge samples taken from two seafood processing plants in Southern Thailand. Their culture broths possessed the ability to flocculate kaolin suspension in the presence of 1% CaCl2. Based on the flocculating activity, the strain S11 was selected and identified to be a Klebsiella sp. using the partial 16S rRNA sequencing method. The growth of the isolated Klebsiella sp. was maximal (1.026 g l-1 dry cell mass) after 1 day cultivation while the highest polymer yield (0.973 g l-1) was achieved after 5 days cultivation. The flocculating activity of the culture broth, however, was highest after 2 days cultivation. The polymer was identified to be an acidic polysaccharide containing neutral sugar and uronic acid as its major and minor components, respectively. Results on the properties of the partially purified polysaccharide from Klebsiella sp. S11 revealed that it consisted of galactose, glucose and mannose in an approximate ratio of 5:2:1. It was soluble in acidic or basic solutions but not in organic solvents. Its molecular mass was greater than 2 x 10(6) Da. Infrared spectra showed the presence of hydroxyl, carboxyl and methoxyl groups in its molecules. Differential scanning calorimetry of the polysaccharide indicated the crystalline melting point (Tm) at 314 degrees C. The optimum dosage of polysaccharide to give the highest flocculating activity was 15 mg l-1 in the presence of 1% CaCl2. PMID:10570817

Dermlim, W; Prasertsan, P; Doelle, H

1999-11-01

375

THE FISCHER-CLIFFORD MATRICES OF THE GROUP 2: SP6(2)  

Microsoft Academic Search

The smallest Fischer sporadic simple group F22 has 14 maximal subgroups up to conjugacy as listed in [2] and [15]. The group 2: SP6(2) is a maximal subgroup of F22 of index 694980. We use the technique of the Fischer-Clifford matrices to constuct the character table of the group 2: SP6(2). The Fischer-Clifford matrices of 2: SP6(2) are used, together

Jamshid Moori; Zwelethemba Mpono

1999-01-01

376

A new microfungal isolate, Embellisia sp., associated with the Antarctic moss Bryum argenteum  

Microsoft Academic Search

A microfungal isolate of Embellisia sp. (Simmons), assigned Embellisia sp2, not previously described from the Antarctic, has been identified by morphological means and internal transcribed spacer\\u000a (ITS) region sequencing. Embellisia sp2 was shown to be associated with the bryophyte Bryum argenteum, collected from Marble Point in Southern Victoria Land, Antarctica, and grew only in samples plated from crushed moss tissue

J. R. Bradner; R. K. Sidhu; B. Yee; M. L. Skotnicki; P. M. Selkirk; K. M. H. Nevalainen

2000-01-01

377

Radioimmunoassay of serum SP 1 and HPL in normal and abnormal pregnancies  

Microsoft Academic Search

Zusammenfassung Zur Bestimmung des schwangerschaftsspezifischen ?1-Glykoproteins (SP 1) wurde ein Radioimmunoassay entwickelt. Die Normalbereiche für SP 1 und das humane Plazenta-Laktogen (HPL) in der zweiten Schwangerschaftshälfte bestimmten wir aus 372 Serumproben von 40 ungestörten Schwangerschaftsverläufen. Der Median von SP 1 stieg von 40 ?g\\/ml in der 22. SSW auf 168 ?g\\/ml in der 36. SSW kontinuierlich an und blieb danach

M. Pluta; W. qHardt; K. Schmidt-Gollwitzer; M. Schmidt-Gollwitzer

1979-01-01

378

Amino Acid Sequences of Porcine Sp38 and Proacrosin Required for Binding to the Zona Pellucida  

Microsoft Academic Search

We previously purified a boar sperm protein, sp38, and demonstrated that this protein bound to the 90-kDa family of zona pellucida (ZP) glycoprotein in a calcium-dependent manner. Sp38 competed with proacrosin for the binding to the zona pellucida. Herein we have isolated cDNA clones encoding sp38 from a boar testis cDNA library in ?gt11. The amino acid sequence deduced from

Etsuko Mori; Shin-ichi Kashiwabara; Tadashi Baba; Yoshimasa Inagaki; Tsuneatsu Mori

1995-01-01

379

Bartonella birtlesii sp. nov., isolated from small mammals (Apodemus spp.).  

PubMed

Three strains isolated from Apodemus spp. were similar to Bartonella species on the basis of phenotypic characteristics. Futhermore, genotypic analysis based on sequence analysis of the 16S rRNA and gltA genes and on DNA-DNA hybridization showed that the three isolates represented a distinct and new species of Bartonella. The name Bartonella birtlesii is proposed for the new species. The type strain of B. birtlesii sp. nov. is IBS 325T (= CIP 106294T = CCUG 44360T). PMID:11155970

Bermond, D; Heller, R; Barrat, F; Delacour, G; Dehio, C; Alliot, A; Monteil, H; Chomel, B; Boulouis, H J; Piémont, Y

2000-11-01

380

Cytotoxic oxylipins from a marine sponge Topsentia sp.  

PubMed

By a bioactivity-guided fractionation, seven new oxylipins, topsentolides A(1)-C(2) (1-7), were isolated from the MeOH extract of a marine sponge Topsentia sp. Detailed NMR and MS analyses established the planar structures of these structurally related oxylipins, which are proposed to be biosynthesized by lipoxygenation followed by cyclization of unsaturated fatty acids. Acetonide derivatives and MTPA esters were prepared to elucidate the stereochemistry of topsentolides B(1) (3), B(2) (4), and C(2) (7). All compounds were tested against a panel of five human solid tumor cell lines and displayed moderate cytotoxicity. PMID:16643027

Luo, Xuan; Li, Famei; Hong, Jongki; Lee, Chong-O; Sim, Chung Ja; Im, Kwang Sik; Jung, Jee H

2006-04-01

381

Complete genome sequence of Thioalkalivibrio sp. K90mix  

PubMed Central

Thioalkalivibrio sp. K90mix is an obligately chemolithoautotrophic, natronophilic sulfur-oxidizing bacterium (SOxB) belonging to the family Ectothiorhodospiraceae within the Gammaproteobacteria. The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method. Here we report the complete genome sequence of strain K90mix and its annotation. The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams. PMID:22675584

Muyzer, Gerard; Sorokin, Dimitry Y.; Mavromatis, Konstantinos; Lapidus, Alla; Foster, Brian; Sun, Hui; Ivanova, Natalia; Pati, Amrita; D'haeseleer, Patrik; Woyke, Tanja; Kyrpides, Nikos C.

2011-01-01

382

Cupriavidus plantarum sp. nov., a plant-associated species.  

PubMed

During a survey of plant-associated bacteria in northeast Mexico, a group of 13 bacteria was isolated from agave, maize and sorghum plants rhizosphere. This group of strains was related to Cupriavidus respiraculi (99.4 %), but a polyphasic investigation based on DNA-DNA hybridization analysis, other genotypic studies and phenotypic features showed that this group of strains actually belongs to a new Cupriavidus species. Consequently, taking all the results together, the description of Cupriavidus plantarum sp. nov. is proposed. PMID:25098225

Estrada-de Los Santos, Paulina; Solano-Rodríguez, Roosivelt; Matsumura-Paz, Lucía Tomiko; Vásquez-Murrieta, María Soledad; Martínez-Aguilar, Lourdes

2014-11-01

383

A new cytosporone derivative from the endophytic fungus Cytospora sp.  

PubMed

Japanese oak wilt (JOW) is a tree disease caused by the fungus Raffaelea quercivora, which is vectored by the ambrosia beetle, Platypus quercivorus. In a screening study of the inhibitory active compounds from fungi, a new cytosporone analogue, compound 1, was isolated from the endophytic fungus Cytospora sp. TT-10 isolated from Japanese oak, together with the known compounds, integracin A (2), cytosporones N (3) and A (4). Their structures were determined by extensive 1D- and 2D-NMR spectroscopic and mass spectral analyses. Compound 1 was identified as 4,5-dihydroxy-3-heptylphthalide and named cytosporone E. Compounds 2 and 3 showed antimicrobial activity against Raffaelea quercivora. PMID:25230507

Takano, Tomoya; Koseki, Takuya; Koyama, Hiromasa; Shiono, Yoshihito

2014-07-01

384

Dolosicoccus paucivorans gen. nov., sp. nov., isolated from human blood.  

PubMed

Phenotypic and phylogenetic studies were performed on a hitherto undescribed Gram-positive, catalase-negative, chain-forming coccus isolated from human blood. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown organism constitutes a new phylogenetic line, close to, but distinct from, Facklamia and Globicatella. The unknown bacterium was readily distinguished from currently recognized Facklamia species and Globicatella sanguinis by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Dolosicoccus paucivorans gen. nov., sp. nov. The type strain of Dolosicoccus paucivorans is CCUG 39307T. PMID:10555324

Collins, M D; Rodriguez Jovita, M; Hutson, R A; Falsen, E; Sjödén, B; Facklam, R R

1999-10-01

385

Oligoneuria macabaiba sp. nov. (Insecta: Ephemeroptera) from Brazil.  

PubMed

The former monotypic genus Oligoneuria Pictet was known solely by a female subimago of the type-species Oligoneuria anomala Pictet. A new species of the genus Oligoneuria from the Atlantic rainforest of Macaé, Rio de Janeiro is described. The description was based in males and females imagos of Oligoneuria macabaiba sp. nov. caught with light traps. This species presents similar wing venation, abdominal posterolateral spines, as well as a membranous extension on anterior portion of the head as seen in the genus. Based on features of the new species, the genus is herein redefined. The species represents the first record of the genus from Southeastern Brazil. PMID:22208073

Gonçalves, Inês C; Da-Silva, Elidiomar R; Nessimian, Jorge L

2011-12-01

386

Triterpene lactones from cultures of Ganoderma sp. KM01.  

PubMed

A revised structure of colossolactone G (1), seven new triterpene lactones, ganodermalactones A-G (2-8), and five known triterpene lactones, colossolactone I (9), schisanlactone B (10), colossolactone B (11), colossolactone E (12), and colossolactone IV (13), and ergosterol have been isolated from cultured biomass of the macrofungi Ganoderma sp. KM01. Their structures were identified by spectroscopic methods. Structures and relative configurations of 3, 7, and 8 were confirmed by X-ray crystallographic analysis. Compounds 7, 10, and 12 exhibited antimalarial activity against Plasmodium falciparum in the range 6.0-10.0 ?M (IC50). PMID:24992637

Lakornwong, Waranya; Kanokmedhakul, Kwanjai; Kanokmedhakul, Somdej; Kongsaeree, Palangpon; Prabpai, Samran; Sibounnavong, Phoutthasone; Soytong, Kasem

2014-07-25

387

Facklamia ignava sp. nov., Isolated from Human Clinical Specimens  

PubMed Central

Two strains of a hitherto-undescribed gram-positive, catalase-negative coccus isolated from human sources were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown strains are genealogically identical and constitute a new line close to, but distinct from, Facklamia hominis. The unknown bacterium was readily distinguished from F. hominis by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Facklamia ignava sp. nov. The type strain of Facklamia ignava is CCUG 37419. PMID:9650988

Collins, Matthew D.; Lawson, Paul A.; Monasterio, Rafael; Falsen, Enevold; Sjöden, Berit; Facklam, Richard R.

1998-01-01

388

Polyketides from a Marine-Derived Fungus Xylariaceae sp.  

PubMed Central

Eighteen polyketides (1–18) including six citrinin derivatives, two phenol derivatives, one cyclopentenone, two naphthol derivatives, and seven tetralone derivatives were isolated from the culture broth of a marine-derived fungal strain Xylariaceae sp. SCSGAF0086. Five of these compounds (1, 2, 8, 9, and 10) were new, and their structures were determined by spectroscopic methods. Compounds 4, 6, 7, and 17 showed enzyme-inhibitory activities towards several tested enzymes, and 6 and 7 showed strong antifouling activity against Bugula neritina larvae settlement. This is the first time that the antifouling and enzyme-inhibitory activities of these compounds has been reported. PMID:23697953

Nong, Xu-Hua; Zheng, Zhi-Hui; Zhang, Xiao-Yong; Lu, Xin-Hua; Qi, Shu-Hua

2013-01-01

389

Sesquiterpenoids from the mangrove-derived endophytic fungus Diaporthe sp.  

PubMed

A new sesquiterpenoid, diaporol A (1), possessing a unique tricyclic lactone framework, eight new drimane sesquiterpenoids, diaporols B-I (2-9), and the known compounds 10 and 11 were isolated from a culture of the mangrove-derived endophyte Diaporthe sp. The absolute configurations of 1-5 were determined by low-temperature (100 K) single-crystal X-ray diffraction with Cu K? radiation. The compounds were evaluated for cytotoxic activity; however, no compound showed significant cytotoxicity against the tested cell lines at a concentration of 20 ?M. PMID:23006147

Zang, Le Yun; Wei, Wei; Guo, Ye; Wang, Ting; Jiao, Rui Hua; Ng, Seik Weng; Tan, Ren Xiang; Ge, Hui Ming

2012-10-26

390

Aspergillus cibarius sp. nov., from traditional meju in Korea.  

PubMed

Aspergillus cibarius sp. nov. isolated from meju, a brick of dried fermented soybeans in Korea, is described. The species was also found from black bean, bread and salami in the Netherlands. It is characterized by abundant yellow to reddish brown ascomata and small lenticular ascospores (4.5-5.5 ?m) with a wide furrow, low equatorial crests and tuberculate or reticulate convex surface. The species was resolved as phylogenetically distinct from the other reported Aspergillus species with an Eurotium teleomorph based on multilocus sequence typing using partial fragments of the ?-tubulin, calmodulin, ITS and RNA polymerase II genes. PMID:22923125

Hong, Seung-Beom; Lee, Mina; Kim, Dae-Ho; Meijer, Martin; Majoor, Eline; Vankuyk, Patricia A; Samson, Robert A

2012-08-01

391

Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803.  

PubMed

Here, we report the photosynthetic production of itaconic acid (IA), a promising building block, from carbon dioxide (CO2) by Synechocystis sp. PCC6803. The engineered PCC6803 strain expressing cis-aconitate decarboxylase, the key enzyme in IA biosynthesis, produced 0.9mg/L and 14.5mg/L of IA at production rates of 42.8?gL(-1)day(-1) and 919.0?gL(-1)day(-1), under conditions of constant bubbling with air and 5% CO2, respectively. This is the first report on the possibility of IA production from CO2 via the photosynthetic process in cyanobacteria. PMID:25554635

Chin, Taejun; Sano, Mei; Takahashi, Tetsuya; Ohara, Hitomi; Aso, Yuji

2015-02-10

392

Bacillus sp. BS061 Suppresses Powdery Mildew and Gray Mold  

PubMed Central

The use of a microorganism, or its secretions, to prevent plant disease offers an attractive alternative or supplement to synthetic fungicides for the management of plant disease without the negative effects of chemical control mechanisms. During a screening for microorganisms with the potential to be used as microbial fungicides, Bacillus sp. BS061 was isolated from a plant leaf. The strain BS061 potently inhibited the mycelial growth of Botrytis cinerea, and significantly reduced disease incidence of powdery mildew in cucumber and strawberry. We also found that the culture filtrate of BS061 inhibited the mycelial growth of various plant pathogens. PMID:23874134

Kim, Young-Sook; Song, Ja-Gyeong; Lee, In-Kyoung; Yeo, Woon-Hyung

2013-01-01

393

Identification of trichostatin derivatives from Streptomyces sp. CPCC 203909.  

PubMed

Four new trichostatin analogues (1-4) and six known analogues have been isolated from the rice fermentation of the Streptomyces sp. CPCC 203909. The structures and absolute configurations of these compounds were determined by extensive spectroscopic analysis including 2D NMR and electronic circular dichroism (ECD) calculations based on the quantum-mechanical time-dependent density functional theory (TDDFT). Compounds 2, 5-7, 9, and 10 up-regulated the transcriptional activity of human high density lipoprotein receptor (CLA-1) with EC50 values of 0.38-78.83?M. PMID:25556102

Chen, Minghua; Wu, Yexiang; He, Yi; Xu, Yanni; Li, Yongzhen; Li, Dongsheng; Feng, Tingting; Yu, Liyan; Hong, Bin; Jiang, Wei; Si, Shuyi

2015-02-01

394

A thermoalkaliphilic lipase of Geobacillus sp. T1  

Microsoft Academic Search

A thermoalkaliphilic T1 lipase gene of Geobacillus sp. strain T1 was overexpressed in pGEX vector in the prokaryotic system. Removal of the signal peptide improved protein\\u000a solubility and promoted the binding of GST moiety to the glutathione-Sepharose column. High-yield purification of T1 lipase\\u000a was achieved through two-step affinity chromatography with a final specific activity and yield of 958.2 U\\/mg and 51.5%,

Thean Chor Leow; Raja Noor Zaliha Raja Abd Rahman; Mahiran Basri; Abu Bakar Salleh

2007-01-01

395

Uranium biosorption by Padina sp. algae biomass: kinetics and thermodynamics  

Microsoft Academic Search

Introduction  Kinetic, thermodynamic, and equilibrium isotherms of the biosorption of uranium ions onto Padina sp., a brown algae biomass, in a batch system have been studied.\\u000a \\u000a \\u000a \\u000a \\u000a Discussion  The kinetic data were found to follow the pseudo-second-order model. Intraparticle diffusion is not the sole rate-controlling\\u000a factor. The equilibrium experimental results were analyzed in terms of Langmuir isotherm depending with temperature. Equilibrium\\u000a data fitted

Mohammad Hassan Khani

396

Metabolism of 4-Chloronitrobenzene by the Yeast Rhodosporidium sp  

PubMed Central

The yeast Rhodosporidium sp. metabolized 4-chloronitrobenzene by a reductive pathway to give 4-chloroacetanilide and 4-chloro-2-hydroxyacetanilide as the major final metabolites. The intermediate production of 4-chloronitrosobenzene, 4-chlorophenylhydroxylamine, and 4-chloroaniline was demonstrated by high-pressure liquid chromatography. Additional studies with selected metabolites established that the metabolite 4-chloro-2-hydroxyacetanilide was produced by an initial Bamberger rearrangement of the hydroxylamine metabolite, followed by acetylation. Direct C hydroxylation of the aromatic ring was not observed in this species. No hydroxamic acid production was detected, even though significant concentrations of the nitroso and hydroxylamine precursors to this functional group were observed. PMID:16345757

Corbett, Michael D.; Corbett, Bernadette R.

1981-01-01

397

Biochemical characterization of Sf 9 Sp-family-like protein factors reveals interesting features  

Microsoft Academic Search

Summary  We earlier documented the involvement of novel Sp-family-like protein factors in transcription from the Autographa californica\\u000a nucleopolyhedrovirus (AcNPV) polyhedrin (polh) gene promoter [Ramachandran et al. (2001) J. Biol. Chem. 276: 23440–23449].\\u000a These zinc-dependent Sp-like factors bind to two putative Sp-factor-binding motifs, present within the AcSp sequence upstream of the polh promoter, with very high affinity (Kd = 2.1 × 10?12?M).

S. Rasheedi; A. Ramachandran; N. Z. Ehtesham; S. E. Hasnain

2007-01-01

398

Lipid Polymorphism Induced by Surfactant Peptide SP-B1-25  

PubMed Central

Pulmonary surfactant protein B (SP-B) is an essential protein for lowering surface tension in the alveoli. SP-B1-25, a peptide comprised of the N-terminal 25 amino-acid residues of SP-B, is known to retain much of the biological activity of SP-B. Circular dichroism has shown that when SP-B1-25 interacts with negatively charged lipid vesicles, it contains significant helical structure for the lipid compositions and peptide/lipid ratios studied here. The effect of SP-B1-25 on lipid organization and polymorphisms was investigated via DSC, dynamic light scattering, transmission electron microscopy, and solid-state NMR spectroscopy. At 1-3 mol% peptide and physiologic temperature, SP-B1-25 partitions at the interface of negatively charged PC/PG lipid bilayers. In lipid mixtures containing 1-5 mol% peptide, the structure of SP-B1-25 remains constant, but 2H and 31P NMR spectra show the presence of an isotropic lipid phase in exchange with the lamellar phase below the Tm of the lipids. This behavior is observed for both DPPC/POPG and POPC/POPG lipid mixtures as well as for both the PC and PG components of the mixtures. For 1-3 mol% SP-B1-25, a return to a single lamellar phase above the lipid mixture Tm is observed, but for 5 mol% SP-B1-25 a significant isotropic component is observed at physiologic temperatures for DPPC and exchange broadening is observed in 2H and 31P NMR spectra of the other lipid components in the two mixtures. DLS and TEM rule out the formation of micellar structures and suggest that SP-B1-25 promotes the formation of a fluid isotropic phase. The ability of SP-B1-25 to fuse lipid lamellae via this mechanism, particularly those enriched in DPPC, suggests a specific role for the highly conserved N-terminus of SP-B in the packing of lipid lamellae into surfactant lamellar bodies or in stabilizing multilayer structures at the air-liquid interface. Importantly, this behavior has not been seen for the other SP-B fragments of SP-B8-25 and SP-B59-80, indicating a critical role for the proline rich first seven amino acids in this protein. PMID:20858421

Farver, R. Suzanne; Mills, Frank D.; Antharam, Vijay C.; Chebukati, Janetricks N.; Fanucci, Gail E.; Long, Joanna R.

2010-01-01

399

Phosphorylation of the transcription factor Sp4 is reduced by NMDA receptor signaling.  

PubMed

The regulation of transcription factor function in response to neuronal activity is important for development and function of the nervous system. The transcription factor Sp4 regulates the developmental patterning of dendrites, contributes to complex processes including learning and memory, and has been linked to psychiatric disorders such as schizophrenia and bipolar disorder. Despite its many roles in the nervous system, the molecular mechanisms regulating Sp4 activity are poorly understood. Here, we report a site of phosphorylation on Sp4 at serine 770 that is decreased in response to membrane depolarization. Inhibition of the voltage-dependent NMDA receptor increased Sp4 phosphorylation. Conversely, stimulation with NMDA reduced the levels of Sp4 phosphorylation, and this was dependent on the protein phosphatase 1/2A. A phosphomimetic substitution at S770 impaired the Sp4-dependent maturation of cerebellar granule neuron primary dendrites, whereas a non-phosphorylatable Sp4 mutant behaved like wild type. These data reveal that transcription factor Sp4 is regulated by NMDA receptor-dependent activation of a protein phosphatase 1/2A signaling pathway. Our findings also suggest that the regulated control of Sp4 activity is an important mechanism governing the developmental patterning of dendrites. PMID:24475768

Saia, Gregory; Lalonde, Jasmin; Sun, Xinxin; Ramos, Belén; Gill, Grace

2014-05-01

400

(GL(2n,C),SP(2n,C)) is a Gelfand Pair  

E-print Network

We prove that (GL_{2n}(C),Sp_{2n}(C)) is a Gelfand pair. More precisely, we show that for an irreducible smooth admissible Frechet representation (\\pi,E) of GL_{2n}(C) the space of continuous functionals Hom_{Sp_{2n}(\\cc)}(E,C) is at most one dimensional. For this we show that any distribution on GL_{2n}(C) invariant with respect to the double action Sp_{2n}(C) \\times Sp_{2n}(C) is transposition invariant. Such a result was previously proven for p-adic fields by M. Heumos and S. Rallis.

Sayag, Eitan

2008-01-01

401

[Plasmid P85 from Azospirillum brasilense SP245: study of the circle of possible hosts and incompatibility with plasmids from Azospirillum brasilense SP7].  

PubMed

The possibility of the stable inheritance of the plasmid p85 mobilized derivatives from Azospirillum brasilense Sp245 in the cells of the bacterial genera Rizobiaceae (Agrobacterium tumfaciens) and Pseudomonadaceae (Pseudomonas putida) has been shown. The plasmid p85 participates in coding for the physiologically active products (the plant hormones). It is not inherited by the Escherichia coli strains. For the first time the incompatibility of azospirillium plasmids has been demonstrated on the example of the plasmid p85 from Azospirillum brasilense Sp245 and the plasmid p115 from Azospirillum brasilense Sp7. PMID:1298886

Katsy, E I

1992-01-01

402

Occurrence of a Thiothrix sp. Attached to Mayfly Larvae and Presence of Parasitic Bacteria in the Thiothrix sp  

PubMed Central

Larvae of the mayfly (Drunella grandis [Eaton]) from Diamond Fork Creek, Utah, were covered with a heavy growth of the sulfide-oxidizing bacterium Thiothrix. The bacterium did not seem to harm the mayfly, but the Thiothrix trichomes were parasitized by three morphologically distinct bacteria, two of which were cytoplasmic and one of which was probably periplasmic. At least two of the parasites destroyed the cytoplasmic contents of the Thiothrix sp., thus killing the host cell. Attempts to obtain the parasites in pure culture were unsuccessful. Images PMID:16348112

Larkin, John M.; Henk, Margaret C.; Burton, Sheril D.

1990-01-01

403

Occurrence of a Thiothrix sp. Attached to Mayfly Larvae and Presence of Parasitic Bacteria in the Thiothrix sp.  

PubMed

Larvae of the mayfly (Drunella grandis [Eaton]) from Diamond Fork Creek, Utah, were covered with a heavy growth of the sulfide-oxidizing bacterium Thiothrix. The bacterium did not seem to harm the mayfly, but the Thiothrix trichomes were parasitized by three morphologically distinct bacteria, two of which were cytoplasmic and one of which was probably periplasmic. At least two of the parasites destroyed the cytoplasmic contents of the Thiothrix sp., thus killing the host cell. Attempts to obtain the parasites in pure culture were unsuccessful. PMID:16348112

Larkin, J M; Henk, M C; Burton, S D

1990-02-01

404

Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov., leaf-associated bacteria isolated from Jatropha curcas L.  

PubMed

Four orange-pigmented isolates, L7-456, L7-484(T), L9-479 and L9-753(T), originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all four isolates belong to the genus Aureimonas. In these analyses, strain L7-484(T) appeared to be most closely related to Aureimonas ureilytica 5715S-12(T) (95.7?% sequence identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753(T) were found to be identical and also shared the highest similarity with A. ureilytica 5715S-12(T) (97.5?%). Both L7-484(T) and L9-753(T) contained Q-10 and Q-9 as predominant ubiquinones and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sulfoquinovosyldiacylglycerol and an aminophospholipid as the major polar lipids. C18?:?1?7c and C16?:?0 were the major fatty acids. Similar to other species in the genus Aureimonas, hydroxylated fatty acids (e.g. C18?:?1 2-OH) and cyclic fatty acids (C19?:?0 cyclo ?8c) were also present. The DNA G+C contents of L7-484(T) and L9-753(T) were 66.1 and 69.4 mol%, respectively. Strains L7-484(T) and L9-753(T) exhibited less than 40?% DNA-DNA hybridization both between themselves and to A. ureilytica KACC 11607(T). Our results support the proposal that strain L7-484(T) represents a novel species within the genus Aureimonas, for which the name Aureimonas jatrophae sp. nov. is proposed, and that strains L9-753(T), L7-456 (?=?KACC 16229 ?=?DSM 25023) and L9-479 (?=?KACC 16228 ?=?DSM 25024) represent a second novel species within the genus, for which the name Aureimonas phyllosphaerae sp. nov. is proposed. The type strains of Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov. are respectively L7-484(T) (?=?KACC 16230(T) ?=?DSM 25025(T)) and L9-753(T) (?=?KACC 16231(T) ?=?DSM 25026(T)). PMID:22922534

Madhaiyan, M; Hu, C J; Jegan Roy, J; Kim, S-J; Weon, H-Y; Kwon, S-W; Ji, L

2013-05-01

405

UNIPASS for AvSP? A Broader View  

NASA Technical Reports Server (NTRS)

UNIPASS is a general-purpose probabilistic computer program consisting of three major modules, including preprocessor, solver and postprocessor. UNIPASS contains a user-friendly Graphical User Interface (GUI), numerous state-of-the-art probabilistic analysis techniques, a large library of statistical distributions and a function module with a large library of support functions that can easily define any complex limit-state function in a scripting FORTRAN-like syntax format. Its inverse probability analysis and sensitivities analysis capabilities make it a powerful design aid in any product cycle. Its precise numerical analysis engine is accurate enough to push the failure probabilities of a design to well below 10 (exp -50). UNIPASS is equipped with advanced artificial intelligence that is designed to handle systems with an essentially unlimited number of random variables with ease and efficiency. Its modular arrangement allows you to tailor an analysis to the desired level of accuracy and efficiency. The depth and comprehensiveness of UNIPASS are built upon the decades of experience and expertise of industry leaders including Boeing Aircraft, NASA and the DoD. Its rich content also makes UNIPASS a valuable instructional tool for random processes and probabilistic mechanics. The topics include: 1) Reliability in AvSP; 2) Role of UNIPASS in AvSP; and 3) Examples. This paper is in viewgraph form.

Wu, N. Eva

2001-01-01

406

Extraction of Zinc from Industrial Waste by a Penicillium sp  

PubMed Central

Zinc was extracted from a filter residue of a copper works (58.6% zinc) by a Penicillium sp. isolated from a metal-containing location. By isotachophoresis citric acid was identified as the leaching agent. Citrate was only formed when the leaching substrate was present. This production of citrate was different in several ways from that achieved by Aspergillus niger: glucose was utilized before fructose; the initial concentration of zinc was 50 to 500 times higher than usual in citrate fermentations with A. niger; citrate production stopped when 80 to 90% of the zinc was leached, although sufficient sugar for further synthesis was still present; and in synthetic media citrate production by A. niger needs an acidic environment (pH 2), while the formation of citric acid by Penicillium sp. occurred in a pH range of 7 to 4. Tests with different concentrations of waste material (0.5, 2.5, and 5%) showed that the highest yield of solubilized zinc occurred with a 2.5% substrate (93% zinc extracted after 13 days). PMID:16347908

Schinner, Franz; Burgstaller, Wolfgang

1989-01-01

407

Biosorption of phenol onto bionanoparticles from Spirulina sp. LEB 18.  

PubMed

The biosorption of phenol onto bionanoparticles from Spirulina sp. LEB 18 was studied. Firstly, the bionanoparticles were prepared from Spirulina sp. strain LEB 18 and characterized. After, response surface methodology was employed to optimize the biosorption process as a function of pH (3.2-8.8) and bionanoparticles dosage (0.15-1.85 g L(-1)). Finally, equilibrium and thermodynamic studies were performed at different temperatures (298-328 K). The bionanoparticles presented hydrodynamic diameter of 232±3 nm and polydispersity index of 0.150. It was found that the more adequate condition for the phenol biosorption was pH of 6.0 and bionanoparticles dosage of 1.85 g L(-1). The Langmuir model presented satisfactory fit with the equilibrium experimental data. The maximum biosorption capacity was 159.33 mg g(-1), obtained at 298 K. The thermodynamic parameters showed that the biosorption was a spontaneous, favorable and exothermic process. Based on these results, it can be affirmed that the bionanoparticles are an alternative, renewable and eco-friendly biosorbent to removal phenol from aqueous solutions. PMID:23849821

Dotto, G L; Gonçalves, J O; Cadaval, T R S; Pinto, L A A

2013-10-01

408

Pseudomonas sp. Strain 273, an Aerobic ?,?-DichloroalkaneDegrading Bacterium  

PubMed Central

A gram-negative, aerobic bacterium was isolated from soil; this bacterium grew in 50% (vol/vol) suspensions of 1,10-dichlorodecane (1,10-DCD) as the sole source of carbon and energy. Phenotypic and small-subunit ribosomal RNA characterizations identified the organism, designated strain 273, as a member of the genus Pseudomonas. After induction with 1,10-DCD, Pseudomonas sp. strain 273 released stoichiometric amounts of chloride from C5 to C12 ?,?-dichloroalkanes in the presence of oxygen. No dehalogenation occurred under anaerobic conditions. The best substrates for dehalogenation and growth were C9 to C12 chloroalkanes. The isolate also grew with nonhalogenated aliphatic compounds, and decane-grown cells dechlorinated 1,10-DCD without a lag phase. In addition, cells grown on decane dechlorinated 1,10-DCD in the presence of chloramphenicol, indicating that the 1,10-DCD-dechlorinating enzyme system was also induced by decane. Other known alkane-degrading Pseudomonas species did not grow with 1,10-DCD as a carbon source. Dechlorination of 1,10-DCD was demonstrated in cell extracts of Pseudomonas sp. strain 273. Cell-free activity was strictly oxygen dependent, and NADH stimulated dechlorination, whereas EDTA had an inhibitory effect. PMID:9726906

Wischnak, Catrin; Löffler, Frank E.; Li, Jieran; Urbance, John W.; Müller, Rudolf

1998-01-01

409

Nitrogen Control of Atrazine Utilization in Pseudomonas sp. Strain ADP  

PubMed Central

Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that support rapid growth of Pseudomonas sp. strain ADP compared to cells cultivated on growth-limiting nitrogen sources. The presence of atrazine in addition to the nitrogen sources did not stimulate degradation. High degradation rates obtained in the presence of ammonium plus the glutamine synthetase inhibitor MSX and also with an Nas? mutant derivative grown on nitrate suggest that nitrogen regulation operates by sensing intracellular levels of some key nitrogen-containing metabolite. Nitrate amendment in soil microcosms resulted in decreased atrazine mineralization by the wild-type strain but not by the Nas? mutant. This suggests that, although nitrogen repression of the atrazine catabolic pathway may have a strong impact on atrazine biodegradation in nitrogen-fertilized soils, the use of selected mutant variants may contribute to overcoming this limitation. PMID:14660340

García-González, Vicente; Govantes, Fernando; Shaw, Liz J.; Burns, Richard G.; Santero, Eduardo

2003-01-01

410

SpIES:The Spitzer IRAC Equatorial Survey  

NASA Astrophysics Data System (ADS)

The Spitzer-IRAC Equatorial Survey, SpIES, is an Exploration Science program using Warm Spitzer to map over 100deg^2 of the SDSS Stripe 82 field, and is the largest extragalactic area surveyed by Spitzer. The primary science drivers are: the measurement of z>3 quasar clustering and the luminosity function in order to test different "AGN feedback'' models; to identify obscured AGN (and take advantage of the wide range of multi-wavelength, multi-epoch ancillary data on the Stripe 82 field); to identify z>6 quasars, and to support other wide-field ancillary science. With our observations very recently completed, we present the first preliminary science results from SpIES. This work is based [in part] on observations made with the Spitzer Space Telescope, which is operated by the Jet Propulsion Laboratory, California Institute of Technology under a contract with NASA. Support for this work was provided by NASA through an award issued by JPL/Caltech.

Timlin, John; Ross, Nicholas; Richards, Gordon T.; Lacy, Mark; Bauer, Franz E.; Brandt, W. Niel; Fan, Xiaohui; Haggard, Daryl; Makler, Martin; Myers, Adam D.; Strauss, Michael A.; Urry, C. Megan; SpIES Team

2015-01-01

411

Microbial transformation of quinoline by a Pseudomonas sp.  

PubMed Central

A Pseudomonas sp. isolated from sewage by enrichment culture on quinoline metabolized this substrate by a novel pathway involving 8-hydroxycoumarin. During early growth of the organism on quinoline, 2-hydroxyquinoline accumulated as the intermediate; 8-hydroxycoumarin accumulated as the major metabolite on further incubation. 2,8-Dihydroxyquinoline and 2,3-dihydroxyphenylpropionic acid were identified as the other intermediates. Inhibition of quinoline metabolism by 1 mM sodium arsenite led to the accumulation of pyruvate, whereas inhibition by 5 mM arsenite resulted in the accumulation of 2-hydroxyquinoline as the major metabolite and 2,8-dihydroxyquinoline as the minor metabolite. Coumarin was not utilized as a growth substrate by this bacterium, but quinoline-grown cells converted it to 2-hydroxyphenylpropionic acid, which was not further metabolized. Quinoline, 2-hydroxyquinoline, 8-hydroxycoumarin, and 2,3-dihydroxyphenylpropionic acid were rapidly oxidized by quinoline-adapted cells, whereas 2,8-dihydroxyquinoline was oxidized very slowly. Quinoline catabolism in this Pseudomonas sp. is therefore initiated by hydroxylation(s) of the molecule followed by cleavage of the pyridine ring to yield 8-hydroxycoumarin, which is further metabolized via 2,3-dihydroxyphenylpropionic acid. PMID:3089153

Shukla, O P

1986-01-01

412

sp3-Bonded silicon allotropes based on the Kelvin problem.  

PubMed

The Kelvin problem, how to partition three-dimensional space into cells of equal volume with minimal area, is a fascinating one. Aggregations of bubbles are naturally physical illustrations of the Kelvin problem. And the superconductor Na8Si46 as an inspiration leads to an amazing discovery of the Weaire-Phelan (WP) structure of foam - the optimal solution to the Kelvin problem to date. Here based on the structural similarity between sp(3)-bonded silicon allotropes and the solutions to the Kelvin problem, a series of new sp(3)-hybridization silicon allotropes, named "Kelvin Silicons", are presented. Furthermore, the structural stability and electronic properties of these new silicon allotropes are investigated using density-functional theory (DFT) calculations. The results show that Kelvin Silicons are structurally stable semiconductors with indirect bandgaps in the range of 0.17-1.40 eV, and their bulk moduli are about 75.9-88.5% that of the diamond phase. The simulated X-ray diffraction spectra of the new silicon crystalline structures would provide more information for possible experimental observations and synthesis. PMID:24036687

Zhao, Hui-Yan; Wang, Jing; Ma, Qing-Min; Liu, Ying

2013-10-28

413

Babesial vector tick defensin against Babesia sp. parasites.  

PubMed

Antimicrobial peptides are major components of host innate immunity, a well-conserved, evolutionarily ancient defensive mechanism. Infectious disease-bearing vector ticks are thought to possess specific defense molecules against the transmitted pathogens that have been acquired during their evolution. We found in the tick Haemaphysalis longicornis a novel parasiticidal peptide named longicin that may have evolved from a common ancestral peptide resembling spider and scorpion toxins. H. longicornis is the primary vector for Babesia sp. parasites in Japan. Longicin also displayed bactericidal and fungicidal properties that resemble those of defensin homologues from invertebrates and vertebrates. Longicin showed a remarkable ability to inhibit the proliferation of merozoites, an erythrocyte blood stage of equine Babesia equi, by killing the parasites. Longicin was localized at the surface of the Babesia sp. parasites, as demonstrated by confocal microscopic analysis. In an in vivo experiment, longicin induced significant reduction of parasitemia in animals infected with the zoonotic and murine B. microti. Moreover, RNA interference data demonstrated that endogenous longicin is able to directly kill the canine B. gibsoni, thus indicating that it may play a role in regulating the vectorial capacity in the vector tick H. longicornis. Theoretically, longicin may serve as a model for the development of chemotherapeutic compounds against tick-borne disease organisms. PMID:17485458

Tsuji, Naotoshi; Battsetseg, Badgar; Boldbaatar, Damdinsuren; Miyoshi, Takeharu; Xuan, Xuenan; Oliver, James H; Fujisaki, Kozo

2007-07-01

414

SP-100 operational life model. Fiscal Year 1990 annual report  

SciTech Connect

This report covers the initial year`s effort in the development of an Operational Life Model (OLM) for the SP-100 Space Reactor Power System. The initial step undertaken in developing the OLM was to review all available documentation from GE on their plans for the OLM and on the degradation and failure mechanisms envisioned for the SP-100. In addition, the DEGRA code developed at JPL, which modelled the degradation of the General Purpose Heat Source based Radioisotope Thermoelectric Generator (GPHS-RTG), was reviewed. Based on the review of the degradation and failure mechanisms, a list of the most pertinent degradation effects along with their key degradation mechanisms was compiled. This was done as a way of separating the mechanisms from the effects and allowing all of the effects to be incorporated into the OLM. The emphasis was on parameters which will tend to change performance as a function of time and not on those that are simply failures without any prior degradation.

Ewell, R.; Awaya, H.

1990-12-14

415

Zygosaccharomyces sapae sp. nov., isolated from Italian traditional balsamic vinegar.  

PubMed

Fourteen yeast isolates were recovered from two traditional balsamic vinegar (TBV) samples collected in the provinces of Modena and Reggio Emilia, Italy. Microsatellite-primed-PCR (MSP-PCR) was used to de-replicate the isolate collection into two representative strains, ABT301(T) and ABT601. Phylogenetic analysis based on the D1/D2 domains of the 26S rRNA gene indicated that these strains represented a distinct species of the genus Zygosaccharomyces, closely related to Zygosaccharomyces rouxii and Zygosaccharomyces mellis. Physiological and morphological tests supported the recognition of a novel taxon of halotolerant, osmotolerant, non-psychrotolerant and maltose-fermentation-negative yeasts showing a chain or star-shaped pattern of budding cells, which remained attached to each other. Morphological observations offered evidence of ascospore formation. A novel species, Zygosaccharomyces sapae sp. nov., is proposed to accommodate these strains, with strain ABT301(T) (= CBS 12607(T) = MUCL 54092(T)) as the type strain. Based on D1/D2 domain phylogenetic analysis, the novel strains shared the highest sequence similarity (100 %) with Zygosaccharomyces sp. strain NCYC 3042, previously isolated from sugar [James, S. A., Bond, C. J., Stratford, M. & Roberts, I. N. (2005). FEMS Yeast Res 5, 747-755]. However, based on phylogenetic (internal transcribed spacers, ITS), PCR fingerprinting and physiological analyses, marked differences were observed between the novel species and strain NCYC 3042, and these results are discussed in more detail. PMID:23024146

Solieri, Lisa; Chand Dakal, Tikam; Giudici, Paolo

2013-01-01

416

Incision at nucleotide insertions/deletions and base pair mismatches by the SP nuclease of spinach.  

PubMed

Spinach leaves contain a highly active nuclease called SP. The purified enzyme incises single-stranded DNA, RNA, and double-stranded DNA that has been destabilized by A-T-rich regions and DNA lesions [Strickland et al. (1991) Biochemistry 30, 9749-9756]. This broad range of activity has suggested that SP may be similar to a family of nucleases represented by S1, P1, and the mung bean nuclease. However, unlike these single-stranded nucleases that require acidic pH and low ionic strength conditions, SP has a neutral pH optimum and is active over a wide range of salt concentrations. We have extended these findings and showed that an outstanding substrate for SP is a mismatched DNA duplex. For base-substitution mismatches, SP incises at all mismatches except those containing a guanine residue. SP also cuts at insertion/deletions of one or more nucleotides. Where the extrahelical DNA loop contains one nucleotide, the preference of extrahelical nucleotide is A > T approximately C but undetectable at G. The inability of SP to cut at guanine residues and the favoring of A-T-rich regions distinguish SP from the CEL I family of neutral pH mismatch endonucleases recently discovered in celery and other plants [Oleykowski et al. (1998) Nucleic Acids Res. 26, 4597-4602]. SP, like CEL I, does not turn over after incision at a mismatched site in vitro. Similar to CEL I, the presence of a DNA polymerase or a DNA ligase allows SP to turn over and stimulate its activity in vitro by about 20-fold. The possibility that the SP nuclease may be a natural variant of the CEL I family of mismatch endonucleases is discussed. PMID:10026304

Oleykowski, C A; Bronson Mullins, C R; Chang, D W; Yeung, A T

1999-02-16

417

Lung Surfactant Protein D (SP-D) Response and Regulation During Acute and Chronic Lung Injury  

PubMed Central

Background Surfactant protein D (SP-D) is a collection that plays important roles in modulating host defense functions and maintaining phospholipid homeostasis in the lung. The aim of current study was to characterize comparatively the SP-D response in bronchoalveolar lavage (BAL) and serum in three murine models of lung injury, using a validated ELISA technology for estimation of SP-D levels. Methods Mice were exposed to lipopolysaccharide, bleomycin, or Pneumocystis carinii (Pc) and sacrificed at different time points. Results In lipopolysaccharide-challenged mice, the level of SP-D in BAL increased within 6 h, peaked at 51 h (4,518 ng/ml), and returned to base level at 99 h (612 ng/ml). Serum levels of SP-D increased immediately (8.6 ng/ml), peaked at 51 h (16 ng/ml), and returned to base levels at 99 h (3.8 ng/ml). In a subacute bleomycin inflammation model, SP-D levels were 4,625 and 367 ng/ml in BAL and serum, respectively, 8 days after exposure. In a chronic Pc inflammation model, the highest level of SP-D was observed 6 weeks after inoculation, with BAL and serum levels of 1,868 and 335 ng/ml, respectively. Conclusions We conclude that serum levels of SP-D increase during lung injury, with a sustained increment during chronic inflammation compared with acute inflammation. A quick upregulation of SP-D in serum in response to acute airway inflammation supports the notion that SP-D translocates from the airways into the vascular system, in favor of being synthesized systemically. The study also confirms the concept of using increased SP-D serum levels as a biomarker of especially chronic airway inflammation. PMID:23435873

Rasmussen, Karina Juhl; Beers, Michael F.; Atochina-Vasserman, Elena N.; Hansen, Soren

2014-01-01

418

Listeria booriae sp. nov, and Listeria newyorkensis sp. nov., from food processing environments in the United States.  

PubMed

Sampling of seafood and dairy processing facilities in the Northeastern United States produced 18 isolates of Listeria spp. that could not be identified to the species-level using traditional phenotypic and genotypic identification methods. Results of phenotypic and genotypic analyses suggest that the isolates comprise two new species with an average nucleotide blast identity of less than 92% with previously described Listeria species. Phylogenetic analyses based on whole genome sequences, 16s rDNA and sigB gene sequences confirmed that the isolates represented by type strain FSL M6-0635T and FSL A5-0209 cluster phylogenetically with L. cornellensis. Phylogenetic analyses also show that the isolates represented by type strain FSL A5-0281 T, cluster phylogenetically with L. riparia. The name L. booriae sp. nov. is proposed for the species represented by type strain FSL A5-0281 T (DSM 28860; LMG 28311) , and the name L. newyorkensis sp. nov. is proposed for the species represented by type strain FSL M6-0635 T (DSM 28861; LMG 28310). Phenotypic and genotypic analyses suggest that neither species is pathogenic. PMID:25342111

Weller, Daniel; Andrus, Alexis; Wiedmann, Martin; den Bakker, Henk

2014-10-23

419

Streptococcus tangierensis sp. nov. and Streptococcus cameli sp. nov., two novel Streptococcus species isolated from raw camel milk in Morocco.  

PubMed

Biochemical and molecular genetic studies were performed on two unidentified Gram-stain positive, catalase and oxidase negative, non-hemolytic Streptococcus-like organisms recovered from raw camel milk in Morocco. Phenotypic characterization and comparative 16S rRNA gene sequencing demonstrated that the two strains were highly different from each other and that they did not correspond to any recognized species of the genus Streptococcus. Phylogenetic analysis based on 16S rRNA gene sequences showed the unidentified organisms each formed a hitherto unknown sub-line within the genus Streptococcus, displaying a close affinity with Streptococcus moroccensis, Streptococcus minor and Streptococcus ovis. DNA G+C content determination, MALDI-TOF mass spectrometry and biochemical tests demonstrated the bacterial isolates represent two novel species. Based on the phenotypic distinctiveness of the new bacteria and molecular genetic evidence, it is proposed to classify the two strains as Streptococcus tangierensis sp. nov., with CCMM B832(T) (=LMG 27683(T)) as the type strain, and Streptococcus cameli sp. nov., with CCMM B834(T) (=LMG 27685(T)) as the type strain. PMID:25491120

Kadri, Zaina; Vandamme, Peter; Ouadghiri, Mouna; Cnockaert, Margo; Aerts, Maarten; Elfahime, El Mostafa; Farricha, Omar El; Swings, Jean; Amar, Mohamed

2014-12-10

420

Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica  

USGS Publications Warehouse

During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales (Tremellomycetes, Agaricomycotina), but did not correspond to any of the hitherto recognized species. Two novel species, Dioszegia antarctica sp. nov. (type strain ANT-03-116T =CBS 10920T =PYCC 5970T) and Dioszegia cryoxerica sp. nov. (type strain ANT-03-071T =CBS 10919T =PYCC 5967T), are described to accommodate ten and three of these strains, respectively. Analysis of ITS sequences demonstrated intrastrain sequence heterogeneity in D. cryoxerica. The latter species is also notable for producing true hyphae with clamp connections and haustoria. However, no sexual structures were observed. The two novel species can be considered obligate psychrophiles, since they failed to grow above 20?°C and grew best between 10 and 15?°C.

Rodriguez, Russell J.; Connell, L.; Redman, R.; Barrett, A.; Iszard, M.; Fonseca, A.

2010-01-01

421

Patterns of relatedness in the Kudoidae with descriptions of Kudoa chaetodoni n. sp. and K. lethrini n. sp. (Myxosporea: Multivalvulida).  

PubMed

Two morphologically novel Kudoa species are characterized from brain tissue of fish, Kudoa chaetodoni n. sp. from Chaetodon unimaculatus (Chaetodontidae) and Kudoa lethrini n. sp. from Gymnocranius audleyi and Lethrinus harak (Lethrinidae). Additionally we characterized a 5-spore valve (SV) Kudoa species from the brain of Sillago ciliata (Sillaginidae). Intriguingly, its 18S rDNA sequence was identical to that of the 7 SV Kudoa yasunagai extracted from the brain of a paralichthyid halibut in Japan. These 2 species may either prove to be con-specific, even though morphology and distribution differ, or demonstrate the limit of specific resolution in the small subunit rDNA gene region. Small subunit rDNA sequences from these new species were used in molecular phylogenetic analyses of kudoids to examine congruence of phylogeny with tissue tropism, geographical distribution, and host specificity. There was significant correlation between tissue tropism in the form of well-supported brain and heart-infecting clades. Host specificity and geographical distribution showed some correlations with genotype. PMID:17234042

Burger, M A A; Cribb, T H; Adlard, R D

2007-05-01

422

Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov., novel thermophilic anaerobic representatives of the phylum Planctomycetes.  

PubMed

Summary. Two novel strains of thermophilic planctomycetes were recovered from terrestrial and subterranean habitats. Strain R1Twas isolated from a hot spring (Kunashir Island, Russia) and the strain SBP2T was isolated from a deep gold mine (South Africa). Both isolates grew in the temperature range from 30oC to 60oC and pH range 5.0-8.0. The isolate R1T grew optimally at 60oC and pH 6.0 - 6.5; for SBP2T optimal conditions were at 52oC and pH 7.5-8.0. Both strains were capable of anaerobic respiration with nitrate and nitrite as electron acceptor as well as of microaerobic growth. They also could grow by fermentation of mono-, di- and polysaccharides. Based on the phylogenetic position and phenotypic features we suggest that new isolates represent two novel species belonging to the new genus of the order Planctomycetales for which the names Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov. are proposed. The type strain of Thermogutta terrifontis, the type species of the genus, is R1T (= DSM 26237T, = VKM B-2805T), the type strain of the Thermogutta hypogea is SBP2T (= JCM19991T=. VKM B-2782T). PMID:25479950

Slobodkina, Galina B; Kovaleva, Olga L; Miroshnichenko, Margarita L; Slobodkin, Alexander I; Kolganova, Tatyana V; Novikov, Andrei A; van Heerden, Esta; Bonch-Osmolovskaya, Elizaveta A

2014-12-01

423

Candida phyllophila sp. nov. and Candida vitiphila sp. nov., two novel yeast species from grape phylloplane in Thailand.  

PubMed

Three strains (K59(T), K60 and K70 (T)) representing two novel yeast species were isolated from the external surface of leaves of different wine grape (Vitis vinifera) plants, which were collected from the Kanchanaburi Research Station (N14°07'15.1? E099°19'05.6?), Wang Dong Sub-district, Mueang District, Kanchanaburi Province, Thailand, by an enrichment technique. The sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene of two strains (K59(T) and K60) were identical and differed from that of strain K70(T). In terms of pairwise sequence similarity of the D1/D2 domain, the closest species to the three strains was Candida asparagi but with 2.3% nucleotide substitutions for strains K59(T) and K60, and 2.1% nucleotide substitutions for strain K70(T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene, the three strains were assigned to be two novel Candida species. Two strains (K59(T) and K60) were assigned as Candida phyllophila sp. nov. (type strain K59(T)=BCC 42662(T)=NBRC 107776(T)=CBS 12671(T)). Candida vitiphila sp. nov. is proposed for strain K70(T) (=BCC 42663(T)=NBRC 107777(T)=CBS 12672(T)). PMID:23863289

Limtong, Savitree; Kaewwichian, Rungluk

2013-01-01

424

Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., with improved definition of Pseudoplatynematum (Ciliophora, Oligohymenophora).  

PubMed

Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., isolated from coastal waters of Qingdao, northern China, were investigated using live observation and silver impregnations. Sathrophilus planus can be recognized by its elongate and conspicuously flattened body, ca. 16 somatic kineties, single postoral kinety, extremely elongated first kinety row of membranelle 1, and its marine habitat. Among these features, the structure of membranelle 1 is the most characteristic and enables this species to be easily distinguished from its congeners. Pseudoplatynematum dengi is characterized by the following features: cell surface conspicuously strengthened and notched; prominent spines both at anterior and posterior ends of cell; membranelles 1 and 2 three-rowed, membranelle 3 single-rowed; paroral membrane composed of two parts, anterior end of upper part extending to about level of membranelle 2; ca. 20 somatic kineties, one postoral kinety; single prolonged caudal cilium; contractile vacuole caudally positioned. The diagnosis of the genus Pseudoplatynematum was renewed to include some new characteristics revealed by silver impregnation methods. PMID:20430600

Fan, Xinpeng; Chen, Xiangrui; Song, Weibo; Al-Rasheid, Khaled A S; Warren, Alan

2010-08-01

425

Graphene-on-diamond devices with increased current-carrying capacity: carbon sp2-on-sp3 technology.  

PubMed

Graphene demonstrated potential for practical applications owing to its excellent electronic and thermal properties. Typical graphene field-effect transistors and interconnects built on conventional SiO(2)/Si substrates reveal the breakdown current density on the order of 1 ?A/nm(2) (i.e., 10(8) A/cm(2)), which is ~100× larger than the fundamental limit for the metals but still smaller than the maximum achieved in carbon nanotubes. We show that by replacing SiO(2) with synthetic diamond, one can substantially increase the current-carrying capacity of graphene to as high as ~18 ?A/nm(2) even at ambient conditions. Our results indicate that graphene's current-induced breakdown is thermally activated. We also found that the current carrying capacity of graphene can be improved not only on the single-crystal diamond substrates but also on an inexpensive ultrananocrystalline diamond, which can be produced in a process compatible with a conventional Si technology. The latter was attributed to the decreased thermal resistance of the ultrananocrystalline diamond layer at elevated temperatures. The obtained results are important for graphene's applications in high-frequency transistors, interconnects, and transparent electrodes and can lead to the new planar sp(2)-on-sp(3) carbon-on-carbon technology. PMID:22329428

Yu, Jie; Liu, Guanxiong; Sumant, Anirudha V; Goyal, Vivek; Balandin, Alexander A

2012-03-14

426

Evidence for a narrow baryonic state decaying to K0Sp and K0Sp¯ in deep inelastic scattering at HERA  

Microsoft Academic Search

A resonance search has been made in the K0Sp and K0Sp¯ invariant-mass spectrum measured with the ZEUS detector at HERA using an integrated luminosity of 121 pb-1. The search was performed in the central rapidity region of inclusive deep inelastic scattering at an ep centre-of-mass energy of 300-318 GeV for exchanged photon virtuality, Q2, above 1 GeV2. Recent results from

S. Chekanov; M. Derrick; J. H. Loizides; S. Magill; S. Miglioranzi; B. Musgrave; J. Repond; R. Yoshida; M. C. K. Mattingly; N. Pavel; P. Antonioli; G. Bari; M. Basile; L. Bellagamba; D. Boscherini; A. Bruni; G. Bruni; G. Cara Romeo; L. Cifarelli; F. Cindolo; A. Contin; M. Corradi; S. de Pasquale; P. Giusti; G. Iacobucci; A. Margotti; A. Montanari; R. Nania; F. Palmonari; A. Pesci; L. Rinaldi; G. Sartorelli; A. Zichichi; G. Aghuzumtsyan; D. Bartsch; I. Brock; S. Goers; H. Hartmann; E. Hilger; P. Irrgang; H.-P. Jakob; O. Kind; U. Meyer; E. Paul; J. Rautenberg; R. Renner; A. Stifutkin; J. Tandler; K. C. Voss; M. Wang; D. S. Bailey; N. H. Brook; J. E. Cole; G. P. Heath; T. Namsoo; S. Robins; M. Wing; M. Capua; A. Mastroberardino; M. Schioppa; G. Susinno; J. Y. Kim; I. T. Lim; K. J. Ma; M. Y. Pac; M. Helbich; Y. Ning; Z. Ren; W. B. Schmidke; F. Sciulli; J. Chwastowski; A. Eskreys; J. Figiel; A. Galas; K. Olkiewicz; P. Stopa; L. Zawiejski; L. Adamczyk; T. Bold; I. Grabowska-Bold; D. Kisielewska; A. M. Kowal; M. Kowal; J. Lukasik; M. Przybycien; L. Suszycki; D. Szuba; J. Szuba; A. Kotanski; W. Slominski; V. Adler; U. Behrens; I. Bloch; K. Borras; V. Chiochia; D. Dannheim; G. Drews; J. Fourletova; U. Fricke; A. Geiser; P. Göttlicher; O. Gutsche; T. Haas; W. Hain; S. Hillert; C. Horn; B. Kahle; U. Kötz; H. Kowalski; G. Kramberger; H. Labes; D. Lelas; H. Lim; B. Löhr; R. Mankel; I.-A. Melzer-Pellmann; C. N. Nguyen; D. Notz; A. E. Nuncio-Quiroz; A. Polini; A. Raval; L. Rurua; U. Schneekloth; U. Stösslein; G. Wolf; C. Youngman; W. Zeuner; S. Schlenstedt; G. Barbagli; E. Gallo; C. Genta; P. G. Pelfer; A. Bamberger; A. Benen; F. Karstens; D. Dobur; N. N. Vlasov; M. Bell; P. J. Bussey; A. T. Doyle; J. Ferrando; S. Hanlon; D. H. Saxon; I. O. Skillicorn; I. Gialas; T. Carli; T. Gosau; U. Holm; N. Krumnack; E. Lohrmann; M. Milite; H. Salehi; P. Schleper; T. Schörner-Sadenius; S. Stonjek; K. Wichmann; K. Wick; A. Ziegler; C. Collins-Tooth; C. Foudas; R. Gonçalo; K. R. Long; A. D. Tapper; P. Cloth; D. Filges; M. Kataoka; K. Nagano; K. Tokushuku; S. Yamada; Y. Yamazaki; A. N. Barakbaev; E. G. Boos; N. S. Pokrovskiy; B. O. Zhautykov; D. Son; K. Piotrzkowski; F. Barreiro; C. Glasman; O. González; L. Labarga; J. del Peso; E. Tassi; J. Terrón; M. Zambrana; M. Barbi; F. Corriveau; S. Gliga; J. Lainesse; S. Padhi; D. G. Stairs; R. Walsh; T. Tsurugai; A. Antonov; P. Danilov; B. A. Dolgoshein; D. Gladkov; V. Sosnovtsev; S. Suchkov; R. K. Dementiev; P. F. Ermolov; I. I. Katkov; L. A. Khein; I. A. Korzhavina; V. A. Kuzmin; B. B. Levchenko; O. Yu. Lukina; A. S. Proskuryakov; L. M. Shcheglova; S. A. Zotkin; I. Abt; C. Büttner; A. Caldwell; X. Liu; J. Sutiak; N. Coppola; S. Grijpink; E. Koffeman; P. Kooijman; E. Maddox; A. Pellegrino; S. Schagen; H. Tiecke; M. Vázquez; L. Wiggers; E. de Wolf; N. Brümmer; B. Bylsma; L. S. Durkin; T. Y. Ling; A. M. Cooper-Sarkar; A. Cottrell; R. C. E. Devenish; B. Foster; G. Grzelak; C. Gwenlan; T. Kohno; S. Patel; P. B. Straub; R. Walczak; A. Bertolin; R. Brugnera; R. Carlin; F. dal Corso; S. Dusini; A. Garfagnini; S. Limentani; A. Longhin; A. Parenti; M. Posocco; L. Stanco; M. Turcato; E. A. Heaphy; F. Metlica; B. Y. Oh; J. J. Whitmore; Y. Iga; G. D'Agostini; G. Marini; A. Nigro; C. Cormack; J. C. Hart; N. A. McCubbin; C. Heusch; I. H. Park; H. Abramowicz; A. Gabareen; S. Kananov; A. Kreisel; A. Levy; M. Kuze; T. Fusayasu; S. Kagawa; T. Tawara; T. Yamashita; R. Hamatsu; T. Hirose; M. Inuzuka; H. Kaji; S. Kitamura; K. Matsuzawa; M. Costa; M. I. Ferrero; V. Monaco; R. Sacchi; A. Solano; M. Arneodo; M. Ruspa; T. Koop; J. F. Martin; A. Mirea; J. M. Butterworth; R. Hall-Wilton; T. W. Jones; M. S. Lightwood; M. R. Sutton; C. Targett-Adams; J. Ciborowski; R. Ciesielski; P. Luzniak; R. J. Nowak; J. M. Pawlak; J. Sztuk; T. Tymieniecka; A. Ukleja; J. Ukleja; A. F. Zarnecki; M. Adamus; P. Plucinski; Y. Eisenberg; D. Hochman; U. Karshon; M. Riveline; A. Everett; L. K. Gladilin; D. Kçira; S. Lammers; L. Li; D. D. Reeder; M. Rosin; P. Ryan; A. A. Savin; W. H. Smith; S. Dhawan; S. Bhadra; C. D. Catterall; S. Fourletov; G. Hartner; S. Menary; M. Soares; J. Standage

2004-01-01

427

Revision of the genus Pteroplistes in India, with the description of two new species Pteroplistes kervasae Jaiswara, n. sp. and Pteroplistes masinagudi Jaiswara, n. sp. (Orthoptera, Grylloidea, Pteroplistinae).  

PubMed

Pteroplistes Brunner von Wattenwyl, 1873 is an Indo-Malaysian cricket genus with only one species, P. platycleis Bolivar, 1899(1900) known from India. Here, we redescribe the genus Pteroplistes and P. platycleis and describe two new Indian species, Pteroplistes kervasae Jaiswara, n. sp. and Pteroplistes masinagudi Jaiswara, n. sp. from the Western Ghats, using morphology and genitalia. Identification keys to separate the Indian species of Pteroplistes are provided and the distribution of the genus in India is discussed. PMID:24943415

Jaiswara, Ranjana; Desutter-Grandcolas, Laure

2014-01-01

428

CpG methylation regulates allelic expression of GDF5 by modulating binding of SP1 and SP3 repressor proteins to the osteoarthritis susceptibility SNP rs143383.  

PubMed

GDF5 encodes an extracellular signalling molecule that is essential for normal skeletal development. The rs144383 C to T SNP located in the 5'UTR of this gene is functional and has a pleiotropic effect on the musculoskeletal system, being a risk factor for knee-osteoarthritis (OA), congenital hip dysplasia, lumbar disc degeneration and Achilles tendon pathology. rs143383 exerts a joint-wide effect on GDF5 expression, with expression of the OA-associated T allele being significantly reduced relative to the C allele, termed allelic expression imbalance. We have previously reported that the GDF5 locus is subject to DNA methylation and that allelic imbalance of rs143383 is mediated by SP1, SP3 and DEAF1 transcriptional repressors. In this study, we have assayed GDF5 methylation in normal and osteoarthritic cartilage, and investigated the effect of methylation on the allelic imbalance of rs143383. We observed demethylation of the GDF5 5'UTR in OA knee cartilage relative to both OA (p = 0.009) and non-OA (p = 0.001) hip cartilage, with the most significant demethylation observed at the highly conserved +37 CpG site located 4 bp upstream of rs143383. Methylation modulates the level and direction of allelic imbalance of rs143383, with methylation of the +37 CpG dinucleotide within the SP1/SP3 binding site having an allele-specific effect on SP1 and SP3 binding. Furthermore, methylation attenuated the repressive effects of SP1, SP3 and DEAF1 on GDF5 promoter activity. This data suggest that the differential methylation of the +37 CpG site between osteoarthritic hip and knee cartilage may be responsible for the knee-specific effect of rs143383 on OA susceptibility. PMID:24861163

Reynard, Louise N; Bui, Catherine; Syddall, Catherine M; Loughlin, John

2014-08-01

429

Structural and electronic properties of new 1D and 2D carbon allotropes with mixed sp1 - sp3 hybridization types  

NASA Astrophysics Data System (ADS)

New 1D and 2D highly porous carbon allotropes are designed, which combine the atoms in sp1 and sp3 hybridization. The structural motifs of suggested compounds can emerge from the same polyyne building blocks as for experimentally fabricated expanded cubane. The structural, electronic properties and relative stability of proposed 1D and 2D carbon allotropes have been examined and discussed through the calculations on the basis of the density functional theory.

Enyashin, A. N.; Ivanovskii, A. L.

2014-08-01

430

Expression of the Nitroarene Dioxygenase Genes in Comamonas sp. Strain JS765 and Acidovorax sp. Strain JS42 Is Induced by Multiple Aromatic Compounds  

Microsoft Academic Search

This work reports a genetic analysis of the expression of nitrobenzene dioxygenase (NBDO) in Comamonas sp. strain JS765 and 2-nitrotoluene dioxygenase (2NTDO) in Acidovorax sp. strain JS42. Strains JS765 and JS42 possess identical LysR-type regulatory proteins, NbzR and NtdR, respectively. NbzR\\/NtdR is homologous to NahR, the positive salicylate-responsive transcriptional activator of the naphthalene degradation genes in Pseudomonas putida G7. The

Daniel J. Lessner; Rebecca E. Parales; Shakti Narayan; David T. Gibson

2003-01-01

431

Three new asexual arthroconidial yeasts, Geotrichum carabidarum sp. nov., Geotrichum histeridarum sp. nov., and Geotrichum cucujoidarum sp. nov., isolated from the gut of insects.  

PubMed

Twenty arthroconidial yeasts were isolated from the digestive tract of basidiome-feeding beetles and lepidopteran larvae. All of the yeasts reproduced only asexually by arthroconidia and some by endo- or blastoconidia as well. Based on the comparisons of sequences in ribosomal RNA genes and other taxonomic characteristics, the yeasts were identified as three unknown Geotrichum species. The three new species are described as Geotrichum carabidarum (NRRL Y-27727T), G. histeridarum (NRRL Y-27729T), and G. cucujoidarum (NRRL Y-27731T). Phylogenetic analyses from ribosomal DNA sequences showed that members of the genus Geotrichum and related arthroconidial yeast taxa were divided into two major clades: (1) Dipodascus and Galactomyces with Geotrichum anamorphs including all the new species; and (2) Magnusiomyces with Saprochaete anamorphs. G. cucujoidarum formed a subclade with G. fermentans and Geotrichum sp. Y-5419, while the two closely related species, G. carabidarum and G. histeridarum, represent a new basal subclade in the clade of Geotrichum and its teleomorphs. PMID:16406553

Suh, Sung-Oui; Blackwell, Meredith

2006-02-01

432

Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus).  

PubMed

Recently, an undescribed Anaplasma sp. (also called Ehrlichia-like sp. or WTD agent) was isolated in ISE6 tick cells from captive white-tailed deer. The goal of the current study was to characterize this organism using a combination of experimental infection, morphologic, serologic, and molecular studies. Each of 6 experimentally inoculated white-tailed deer fawns (Odocoileus virginianus) became chronically infected (100+ days) with the Anaplasma sp. by inoculation of either infected whole blood or culture. None of the deer showed evidence of clinical disease, but 3 of the 6 deer evaluated had multiple episodes of transient thrombocytopenia. Light microscopy of Giemsa-stained, thin blood smears revealed tiny, dark, spherical structures in platelets of acutely infected deer. Anaplasma sp. was detected in platelets of inoculated deer by polymerase chain reaction, transmission electron microscopy, immunohistochemistry, and in situ hybridization. Five of 6 deer developed antibodies reactive to Anaplasma sp. antigen, as detected by indirect fluorescent antibody testing. Phylogenetic analyses of 16S rRNA, groESL, and gltA sequences confirmed the Anaplasma sp. is related to A. platys. Two attempts to transmit the Anaplasma sp. between deer by feeding Amblyomma americanum, a suspected tick vector, were unsuccessful. Based on its biologic, antigenic, and genetic characteristics, this organism is considered a novel species of Anaplasma, and the name Anaplasma odocoilei sp. nov. is proposed with UMUM76(T) (=CSUR-A1) as the type strain. PMID:23276749

Tate, Cynthia M; Howerth, Elizabeth W; Mead, Daniel G; Dugan, Vivien G; Luttrell, M Page; Sahora, Alexandra I; Munderloh, Ulrike G; Davidson, William R; Yabsley, Michael J

2013-02-01

433

Draft Genome of Janthinobacterium sp. RA13 Isolated from Lake Washington Sediment  

PubMed Central

Sequencing the genome of Janthinobacterium sp. RA13 from Lake Washington sediment is announced. From the genome content, a versatile life-style is predicted, but not bona fide methylotrophy. With the availability of its genomic sequence, Janthinobacterium sp. RA13 presents a prospective model for studying microbial communities in lake sediments. PMID:25676775

McTaggart, Tami L.; Shapiro, Nicole; Woyke, Tanja

2015-01-01

434

Draft Genome of Pseudomonas sp. Strain 11/12A, Isolated from Lake Washington Sediment  

PubMed Central

We announce here the genome sequencing of Pseudomonas sp. strain 11/12A from Lake Washington sediment. From the genome content, a versatile lifestyle is predicted but not one of bona fide methylotrophy. With the availability of its genomic sequence, Pseudomonas sp. 11/12A presents a prospective model for studying microbial communities in lake sediments.

McTaggart, Tami L.; Shapiro, Nicole; Woyke, Tanja

2015-01-01

435

Correlating S&P 500 Stocks with Twitter Data University of Connecticut  

E-print Network

Correlating S&P 500 Stocks with Twitter Data Yuexin Mao University of Connecticut yuexin with exogenous input model to pre- dict stock market indicators, using Twitter data as exoge- nous input. Our investigate whether the daily number of tweets that mention Standard & Poor 500 (S&P 500) stocks is corre

Wang, Bing

436

The influence of salinity variation on Hematodinium sp. infection of blue crabs in the ACE Basin  

E-print Network

The influence of salinity variation on Hematodinium sp. infection of blue crabs in the ACE Basin measured salinity, crab abundance, fishing effort, and Hematodinium sp. prevalence at 27 locations and by river. High salinity in September leads to high infection in December, but variation in infection among

Childress, Michael J.

437

New Pigments from the Terrestrial Cyanobacterium Scytonema sp. Collected on the Mitaraka Inselberg, French Guyana  

E-print Network

1 New Pigments from the Terrestrial Cyanobacterium Scytonema sp. Collected on the Mitaraka of the ultraviolet-screening, photostable sheath pigment scytonemin. The organic extract of Scytonema sp., collected on the Mitaraka inselberg, French Guyana, yielded three new pigments, tetramethoxyscytonemin (1

Paris-Sud XI, Université de

438

1 Estimating aquifer hydraulic properties from the inversion of surface 2 Streaming Potential (SP) anomalies  

E-print Network

1 Estimating aquifer hydraulic properties from the inversion of surface 2 Streaming Potential (SP with the geometry of the water table. It follows that 11 SP measurements can be used to estimate aquifer hydraulic and found that we 14 are able to estimate the hydraulic conductivity and the depth 15 and the thickness

Sailhac, Pascal

439

SP-A Preserves Airway Homeostasis During Mycoplasma pneumoniae Infection in Mice  

PubMed Central

The lung is constantly challenged during normal breathing by a myriad of environmental irritants and infectious insults. Pulmonary host defense mechanisms maintain homeostasis between inhibition/clearance of pathogens and regulation of inflammatory responses that could injure the airway epithelium. One component of this defense mechanism, surfactant protein-A (SP-A), exerts multifunctional roles in mediating host responses to inflammatory and infectious agents. SP-A has a bacteriostatic effect on Mycoplasma pneumoniae (Mp), which occurs by binding surface disaturated phosphatidylglycerols. SP-A can also bind the Mp membrane protein, MPN372. In this study we investigated the role of SP-A during acute phase pulmonary infection with Mp using mice deficient in SP-A. Biologic responses, inflammation and cellular infiltration, were much greater in Mp infected SP-A?/? mice than wild type mice. Likewise, physiologic responses (airway hyperresponsiveness and lung compliance) to Mp infection were more severely affected in SP-A?/? mice. Both Mp-induced biologic and physiologic changes were attenuated by pharmacologic inhibition of TNF-?. Our findings demonstrate that SP-A is vital to preserving lung homeostasis and host defense to this clinically relevant strain of Mp by curtailing inflammatory cell recruitment and limiting an overzealous TNF-? response. PMID:19494306

Ledford, Julie G.; Goto, Hisatsugu; Potts, Erin N.; Degan, Simone; Chu, Hong Wei; Voelker, Dennis R.; Sunday, Mary E.; Cianciolo, George J.; Foster, William M.; Kraft, Monica; Wright, Jo Rae

2013-01-01

440

Draft Genome Sequence of Geobacillus sp. Strain FW23, Isolated from a Formation Water Sample  

PubMed Central

The thermophilic Geobacillus sp. strain FW23 was isolated from the Mehsana oil wells in Gujrat, India, during a screening for oil-degrading bacteria. Here, we report the draft genome sequence of Geobacillus sp. FW23, which may help reveal the genomic differences between this strain and the earlier reported species of the genus Geobacillus. PMID:24812215

Pore, Soham D.; Arora, Preeti

2014-01-01

441

Graphene-on-Diamond Devices with Increased Current-Carrying Capacity: Carbon sp2  

E-print Network

Graphene-on-Diamond Devices with Increased Current-Carrying Capacity: Carbon sp2 -on-sp3 Technology Laboratory, Illinois 60439, United States *S Supporting Information ABSTRACT: Graphene demonstrated potential for practical applications owing to its excellent electronic and thermal properties. Typical graphene field

442

Draft Genome Sequence of the Alga-Aggregating Bacterium Bacillus sp. Strain RP1137  

PubMed Central

Bacillus sp. strain RP1137 is a bacterium that is able to rapidly and efficiently aggregate biofuel-producing microalgae. By 16S rRNA gene sequencing, it was found to be related to the industrially important Bacillus megaterium. Here, we report the draft genome sequence of Bacillus sp. strain RP1137. PMID:24385572

Powell, Ryan J.; Bachvaroff, Tsvetan R.

2014-01-01

443

Genome Sequence of Ralstonia sp. Strain PBA, a Bacterium Involved in the Biodegradation of 4-Aminobenzenesulfonate  

PubMed Central

Ralstonia sp. strain PBA was isolated from textile wastewater in a coculture with Hydrogenophaga sp. strain PBC. Here we present the assembly and annotation of its genome, which may provide further insights into the mechanism of its interaction with strain PBC during 4-aminobenzenesulfonate degradation. PMID:22933765

Chew, Teong Han; Tay, Yea-Ling; Lye, Siew Fen; Yahya, Adibah

2012-01-01

444

Transpiration of the 'Rebula' cuttings (Vitis vinifera L.) grafted on three different rootstocks (Vitis sp.)  

Microsoft Academic Search

The purpose of the investigation was to determine the use of water in grafted vines (Vitis vinifera L.) in dependence on the vine (Vitis sp.) rootstocks. The local vine variety 'Rebula' was grafted on three different rootstocks (Vitis sp.): (1) on the 'Rebula' (Vitis vinifera L.) vine itself, (2) on the rootstock 196\\/17 Castel' ((Vitis vinifera 'Mourvèdre' x Vitis rupestris

Paolo SIVILOTTI; Enrico PETERLUNGER

445

Performance of NAS parallel application-benchmarks on IBM SP1  

Microsoft Academic Search

We present the performance of three application benchmarks (BT, LU, and SP) from the NAS Parallel Benchmark suite on the IBM scalable POWERparallel 1 (SP1) system. We present performance results on both Class A and Class B problem sets. We compare the performance of two communication protocols, MPL and MPL\\/p, and also present results on a cluster of workstations called

Vijay K. Naik

1994-01-01

446

Disease development and genotypic diversity of Puccinia graminis f. sp. avenae in Swedish oat fields  

Technology Transfer Automated Retrieval System (TEKTRAN)

The disease development and population structure of Puccinia graminis f. sp. avenae, which causes stem rust on oat, were studied to investigate if sexual reproduction plays an important role in the epidemiology of the disease. The genetic population structure of P. graminis f. sp. avenae in Sweden w...

447

Feature Article A Microbial Biosensor for p-Nitrophenol Using Arthrobacter Sp.  

E-print Network

Feature Article A Microbial Biosensor for p-Nitrophenol Using Arthrobacter Sp. Yu Lei,a Priti biosensor for measurement of p-nitrophenol (PNP), a U.S. Environmental Agency priority pollutant. The biosensor consisted of PNP-degrading/oxidizing bacteria Arthrobacter sp. JS443 as biological sensing element

Chen, Wilfred

448

Immunosuppression by human placenta lactogen (HPL) and the pregnancy-specific ? 1 -glycoprotein (SP1)  

Microsoft Academic Search

The in vitro effect of human placenta lactogen (HPL) and the gravidity-specificß1-Glycoprotein (SP-1) on the lymphocyte transformation was investigated in 20 healthy women. It was shown that simultaneous incubation of the lymphocytes with HPL or SP-1 together with PHA has no influence on the incorporation rate of H3-Thymidine.

C. Cerni; G. Tatra; H. Bohn

1977-01-01

449

Concentrations of placental proteins (HPL and SP1) in maternal serum throughout normal pregnancy  

Microsoft Academic Search

Summary Throughout 65 normal singleton pregnancies 332 blood samples were obtained and analyzed for pregnancy-specific ?1 glycoprotein (SP1) and human placental lactogen (HPL). The measurement of SP1 in maternal serum was made using radial immunodiffusion, that of HPL by using radioimmunoassay. There was wide variation in the number and timing of blood samples obtained from patients, and therefore the results

M. P. Baur; O. Bellmann; J. Tebbe; N. Lang

1982-01-01

450

Localization of the Sperm Protein SP22 and Inhibition of Fertility In Vivo and In Vitro  

Microsoft Academic Search

We previously established that levels of the sperm membrane protein, SP22, are highly correlated with the fertility of sperm from the cauda epididymidis of rats exposed to both epidid- ymal and testicular toxicants, and that a testis-specific SP22 tran- script is expressed in postmeiotic germ cells. In this study, polyclonal and monoclonal antibodies were generated to study the expression of

GARY R. KLINEFELTER; JEFFREY E. WELCH; SALLY D. PERREAULT; HARRY D. MOORE; ROBERT M. ZUCKER; JUAN D. SUAREZ; NAOMI L. ROBERTS; KATHY BOBSEINE; SUSAN JEFFAY

2002-01-01

451

Draft Genome Sequences of Vibrio sp. Strains Isolated from Tetrodotoxin-Bearing Scavenging Gastropod  

PubMed Central

Vibrio sp. strains JCM 18905 and JCM 19053 were isolated from a tetrodotoxin (TTX)-bearing scavenging gastropod, and Vibrio sp. strain JCM 18904 was isolated from a sea cucumber. All these are closely related to Vibrio alginolyticus. Their comparative genome information is useful for studies of TTX production in bacteria. PMID:24948773

Kawauchi, Ayumi; Nakahara, Tomomi; Zhang, Xiaochi; Taniyama, Shigeto; Takatani, Tomohiro; Arakawa, Osamu; Oshima, Kenshiro; Suda, Wataru; Kitamura, Keiko; Iida, Toshiya; Iino, Takao; Inoue, Tetsushi; Hongoh, Yuichi; Hattori, Masahira

2014-01-01

452

Role of the N-Terminal Seven Residues of Surfactant Protein B (SP-B)  

PubMed Central

Breathing is enabled by lung surfactant, a mixture of proteins and lipids that forms a surface-active layer and reduces surface tension at the air-water interface in lungs. Surfactant protein B (SP-B) is an essential component of lung surfactant. In this study we probe the mechanism underlying the important functional contributions made by the N-terminal 7 residues of SP-B, a region sometimes called the “insertion sequence”. These studies employed a construct of SP-B, SP-B (1–25,63–78), also called Super Mini-B, which is a 41-residue peptide with internal disulfide bonds comprising the N-terminal 7-residue insertion sequence and the N- and C-terminal helices of SP-B. Circular dichroism, solution NMR, and solid state 2H NMR were used to study the structure of SP-B (1–25,63–78) and its interactions with phospholipid bilayers. Comparison of results for SP-B (8–25,63–78) and SP-B (1–25,63–78) demonstrates that the presence of the 7-residue insertion sequence induces substantial disorder near the centre of the lipid bilayer, but without a major disruption of the overall mechanical orientation of the bilayers. This observation suggests the insertion sequence is unlikely to penetrate deeply into the bilayer. The 7-residue insertion sequence substantially increases the solution NMR linewidths, most likely due to an increase in global dynamics. PMID:24023779

Sharifahmadian, Mahzad; Sarker, Muzaddid; Palleboina, Dharamaraju; Waring, Alan J.; Walther, Frans J.; Morrow, Michael R.; Booth, Valerie

2013-01-01

453

Cognitive-Behavioral Therapy for Suicide Prevention (CBT-SP): Treatment Model, Feasibility, and Acceptability  

ERIC Educational Resources Information Center

Objective: To describe the elements of a manual-based cognitive-behavioral therapy for suicide prevention (CBT-SP) and to report its feasibility in preventing the recurrence of suicidal behavior in adolescents who have recently attempted suicide. Method: The CBT-SP was developed using a risk reduction and relapse prevention approach and…

Stanley, Barbara; Brown, Gregory; Brent, David A.; Wells, Karen; Poling, Kim; Curry, John; Kennard, Betsy D.; Wagner, Ann; Cwik, Mary F.; Klomek, Anat Brunstein; Goldstein, Tina; Vitiello, Benedetto; Barnett, Shannon; Daniel, Stephanie; Hughes, Jennifer

2009-01-01

454

A novel thermoalkaliphilic xylanase from Gordonia sp. is salt, solvent and surfactant tolerant.  

PubMed

Two aerobic bacterial consortia namely Con T and Con R were developed by enrichment technique from termite gut and cow dung respectively, using xylan as a sole carbon source. Molecular characterization of Con R based on 16S rRNA sequence analysis showed the presence of Pannonibacter sp. R-3 and Pseudoxanthomas sp. R-5. On the other hand, Con T showed the presence of Pseudoxanthomas sp. T-5, Cellulosimicrobium sp. T-21, and Gordonia sp. T-30. Being the maximum xylanase producer among the five isolates and being a novel xylanase producing bacterial genus, Gordonia sp. T-30 was selected. Xylanase produced by Gordonia sp. T-30 showed optimum activity at 60?°C and pH 9. Xylanase was 95% stable for 120?min at pH 9.0 and 98% stable at 60?°C for 90?min. Xylanase activity was stimulated in the presence of organic solvents such as petroleum ether, acetone, diethyl ether, n-hexane, and benzene. Detergent like cetyltrimethylammonium bromide and presence of NaCl also accelerated the xylanase function. Comparative evaluation was studied between sterilized and non-sterilized solid fermentation to produce xylanase by Gordonia sp. T-30 using various agricultural residues as growth substrate in cost effective manner. Industrially important features endowed by this xylanase make it a very promising candidate for food, feed, and fuel industry. PMID:24912946

Kashyap, Radhika; Monika; Subudhi, Enketeswara

2014-12-01

455

SP3X : a six-degree of freedom device for natural model creation  

E-print Network

This thesis presents a novel input device, called SP3X, for the creation of digital models in a semi-immersive environment. The goal of SP3X is to enable novice users to construct geometrically complex three-dimensional ...

Whitney, Richard Henry, III

2007-01-01

456

Potassium uptake in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 mainly depends on  

E-print Network

Potassium uptake in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 mainly depends First published online 3 July 2003 Edited by Stuart Ferguson Abstract The molecular basis of potassium potassium transporters can be identi¢ed in the genome of Synechocystis sp. strain PCC 6803. Mutants

Roegner, Matthias

457

Draft Genome Sequence of Geobacillus sp. Strain FW23, Isolated from a Formation Water Sample.  

PubMed

The thermophilic Geobacillus sp. strain FW23 was isolated from the Mehsana oil wells in Gujrat, India, during a screening for oil-degrading bacteria. Here, we report the draft genome sequence of Geobacillus sp. FW23, which may help reveal the genomic differences between this strain and the earlier reported species of the genus Geobacillus. PMID:24812215

Pore, Soham D; Arora, Preeti; Dhakephalkar, Prashant K

2014-01-01

458

LOCALIZATION OF THE SPERM PROTEIN SP22 AND INHIBITION OF FERTILITY IN VIVO AND IN VITRO  

EPA Science Inventory

We previously established that the levels sperm membrane protein SP22 are highly correlated with the fertility of sperm from the cauda epididymidis of rats exposed to both epididymal and testicular toxicants, and that a testis-specific SP22 transcript is expressed in post-meiotic...

459

The application of SP100 technology in a lunar surface power system  

Microsoft Academic Search

Consideration is given to a conceptual design study addressing the application of the SP-100 space reactor power system technology to a lunar surface power system (LSPS). Data obtained demonstrate that the SP-100 technology can be readily adapted to the lunar environment to meet the load demands of the lunar outpost. Particular attention is given to the conceptual design for a

H. A. Upton; R. Protsik; R. E. Gamble; R. L. Otwell; D. W. Lunsford

1991-01-01

460

SP100 nuclear assembly test - Test assembly functional requirements and system arrangement  

Microsoft Academic Search

This paper describes the functional requirements and the system that will be tested to validate the reactor, flight shield, and flight controller of the SP-100 Generic Flight System. The Nuclear Assembly Test consists of the test article (SP-100 reactor with control devices and the flight shield) and its supporting systems.

T. T. Fallas; Robert Gluck; Kumar Motwani; Harold Clay; Gerald O'Neill

1991-01-01