Sample records for sp metabolitos secundarios

  1. Desarrollo de un Método de Análisis de Metabolitos de Nitrofuranos en Tejido Animal Empleando el Sistema GC\\/PTV\\/EI\\/MSMS

    Microsoft Academic Search

    Sonia del Carmen; Soto Alvarado; Osvaldo Nicolás; Rodríguez Valdés; Guillermo Rodríguez; Ernesto Vélez López; Juan Temores Peña; Laura Renné; Velázquez Chong

    2008-01-01

    RESUMEN En el análisis de metabolitos de 5-nitrofuranos actualmente se emplean sistemas en fase líquida, acoplados a analizadores de masas\\/masas LC\\/MSMS los cuales son muy costosos. En este estudio se presenta un método alternativo en el cual se utiliza un sistema en fase gaseosa, con analizador de masa secuencial de trampa de iones y un sistema de inyección de temperatura

  2. SP Fonts

    NSDL National Science Digital Library

    If you're looking for funky fonts to include in Web designs, papers, or posters, SP Fonts may be your answer. Scholars Press (SP) Fonts "are a set of simple, public domain fonts" designed for print and non-commercial Web use. This site currently offers eight public domain fonts that may be downloaded and used free of charge. Three of the fonts are Hebrew/Aramaic fonts: "SPTiberian (a standard Hebrew font), SPDamascus (a thinner font with Palestinian as well as Tiberian vowel points), and SPEzra (a simple, fixed-width Hebrew font)." Two are Greek fonts: "SPIonic (a more complete Greek font) and SPDoric (a simpler, uncial font)." Other fonts include "SPEdessa (a Syriac Estrangela font), SPAchmim (a Coptic font), and SPAtlantis (a transliteration font that includes diacriticals and other special characters that allow the representation of numerous Indo-European, Semitic, and other languages"-- available in both Roman and Italic type). All fonts are TrueType fonts and are compatible with PC (Windows) and Mac computers. In addition, each font has a .readme file that explains the standard keyboard mapping used by the font. Although the fonts are free to the public, the Web site requests permission from the copyright holder before including the typefaces in commercial electronic products.

  3. Sp1-mediated transcriptional activation is repressed by Sp3.

    PubMed Central

    Hagen, G; Müller, S; Beato, M; Suske, G

    1994-01-01

    Sp1, Sp3 (SPR-2) and Sp4 (SPR-1) are human sequence-specific DNA binding proteins with very similar structural features. In this report, we have analyzed Sp3 in direct comparison with Sp1. We have raised antibodies against both Sp1 and Sp3, and show that Sp3 protein, like Sp1, is expressed in various cell lines. Co-transfection experiments in different mammalian cell lines reveal that in contrast to Sp1 and Sp4, Sp3 is not able to activate several Sp1 responsive promoters. In addition, Sp3 also fails to activate reporter constructs in Drosophila SL2 cells lacking endogenous Sp factors. Instead, we find that Sp3 represses Sp1-mediated activation in a linear dose-dependent manner. A mutant of Sp3 lacking the DNA binding domain does not affect activation by Sp1, suggesting that the inhibition is most likely due to the competition with Sp1 for their common binding sites. To determine if any structurally similar domain of Sp3 is able to replace partially homologous domains of Sp1, we have generated chimeric proteins and tested their activation characteristics in gene transfer experiments. It appears that neither the glutamine-rich domains A and B nor the D domain of Sp1 can be replaced by the homologous regions of Sp3. Our results suggest that Sp3 is an inhibitory member of the Sp family. Images PMID:8070411

  4. UTILIZACIÓN DE RESIDUOS DE PLÁTANO PARA LA PRODUCCIÓN DE METABOLITOS SECUNDARIOS POR FERMENTACIÓN EN ESTADO SÓLIDO CON EL HONGO Lentinus crinitus UTILIZATION OF PLANTAIN WASTE FOR THE PRODUCTION OF SECONDARY ME TABOLITES BY SOLID SUBSTRATE FERMENTATION USING THE FUNGI Lentinus crinitus

    Microsoft Academic Search

    GRANDA R. Diana; JIMÉNEZ T. Gloria; REVISTA DE LA FACULTAD DE QUÍMICA

    2005-01-01

    This paper presents the production of secondary metabolites with pharmaceutical and alimentary interest from plantain waste used as substrate in a solid state fermentation process with the white root fungi Lentinus crinitus . The production of metabolites is studied during the growth of the microorganism for a period of 21 days, in a system con-

  5. Gaultheria sp. (Native) 

    E-print Network

    James R. Manhart

    2011-08-10

    designers would make small, natural- looking designs. Since the "Primavera" containers were a plastic shell with floral foam inside, they did not look very natural so we had to use lemonleaf (Gaultheria, sp. ), other leaves, different kinds of mosses...

  6. SP2007 Teamrobotik Daniel Meyer,

    E-print Network

    SP2007 Teamrobotik Daniel Meyer, Nils M¨uller & Felix Penzlin SP2007 Teamrobotik Lego Mindstorms Java OpenSource erfordert keinen Mindstorms-Treiber keine eigene IDE Status: Alpha Daniel Meyer, Nils M

  7. Sp(2)-BRST

    SciTech Connect

    Twisk, S.; Zhang, R.B.

    1988-09-01

    A general method is given for the construction of gauge-fixed actions for theories with local gauge symmetries. The method is based on the single requirement that the space of fields carries an irreducible representation of the Sp(2)-BRST algebra, with respect to which the resultant actions are then automatically invariant.

  8. SP-100 surety evaluation

    NASA Astrophysics Data System (ADS)

    1985-06-01

    This report describes surety evaluations conducted during GFY 1985 in support of the General Electric design for a Space Nuclear Power System - SP-100. Those surety evaluations address both safety and safeguards requirements, which are derived from OSNP-1 and supporting documents. The report includes results of neutronics (criticality) calculations performed by Los Alamos. The results have been benchmarked against independent calculations performed by General Electric with different codes. These comparisons show close agreement, and are summarized. Los Alamos has also provided specifications of explosion and fire environments, which have been used in evaluation of the GE SP-100 concept. Following the summary of key results, surety requirements are given and recommendations toward specification of requirements for later SP-100 project phases are presented. A conceptual design summary is presented. To establish a comprehensive background for surety evaluations, a reference mission profile and potential accidents for each phase of the mission are identified. The main body of the report addresses surety of the General Electric Thermoelectric Conversion design. GE has also developed a Stirling Engine concept, and performed comprehensive surety evaluations for it. These evaluations are reported.

  9. SP mountain data analysis

    NASA Astrophysics Data System (ADS)

    Rawson, R. F.; Hamilton, R. E.; Liskow, C. L.; Dias, A. R.; Jackson, P. L.

    1981-09-01

    An analysis of synthetic aperture radar data of SP Mountain was undertaken to demonstrate the use of digital image processing techniques to aid in geologic interpretation of SAR data. These data were collected with the ERIM X- and L-band airborne SAR using like- and cross-polarizations. The resulting signal films were used to produce computer compatible tapes, from which four-channel imagery was generated. Slant range-to-ground range and range-azimuth-scale corrections were made in order to facilitate image registration; intensity corrections were also made. Manual interpretation of the imagery showed that L-band represented the geology of the area better than X-band. Several differences between the various images were also noted. Further digital analysis of the corrected data was done for enhancement purposes. This analysis included application of an MSS differencing routine and development of a routine for removal of relief displacement. It was found that accurate registration of the SAR channels is critical to the effectiveness of the differencing routine. Use of the relief displacement algorithm on the SP Mountain data demonstrated the feasibility of the technique.

  10. SP mountain data analysis

    NASA Technical Reports Server (NTRS)

    Rawson, R. F.; Hamilton, R. E.; Liskow, C. L.; Dias, A. R.; Jackson, P. L.

    1981-01-01

    An analysis of synthetic aperture radar data of SP Mountain was undertaken to demonstrate the use of digital image processing techniques to aid in geologic interpretation of SAR data. These data were collected with the ERIM X- and L-band airborne SAR using like- and cross-polarizations. The resulting signal films were used to produce computer compatible tapes, from which four-channel imagery was generated. Slant range-to-ground range and range-azimuth-scale corrections were made in order to facilitate image registration; intensity corrections were also made. Manual interpretation of the imagery showed that L-band represented the geology of the area better than X-band. Several differences between the various images were also noted. Further digital analysis of the corrected data was done for enhancement purposes. This analysis included application of an MSS differencing routine and development of a routine for removal of relief displacement. It was found that accurate registration of the SAR channels is critical to the effectiveness of the differencing routine. Use of the relief displacement algorithm on the SP Mountain data demonstrated the feasibility of the technique.

  11. Laser sculpting of atomic sp, sp(2) , and sp(3) hybrid orbitals.

    PubMed

    Liu, Chunmei; Manz, Jörn; Yang, Yonggang

    2015-01-12

    Atomic sp, sp(2) , and sp(3) hybrid orbitals were introduced by Linus Pauling to explain the nature of the chemical bond. Quantum dynamics simulations show that they can be sculpted by means of a selective series of coherent laser pulses, starting from the 1s orbital of the hydrogen atom. Laser hybridization generates atoms with state-selective electric dipoles, opening up new possibilities for the study of chemical reaction dynamics and heterogeneous catalysis. PMID:25257703

  12. NASA SP-4009 APOLLO SPACECRAFT

    E-print Network

    Rathbun, Julie A.

    NASA SP-4009 THE APOLLO SPACECRAFT VOLUME IV January 21, 1966-July 13, 1974 hy Ivan D. Ertel (revised) Main entry under title: The Apollo spacecraft. (The NASA historical series) (NASA SP-4009. 8, 1962-Sept. 30, 1964. [etc.] Includes bibliographical references. 1. Project Apollo. I. Ertel

  13. Acetobacter intermedius, sp. nov.

    PubMed

    Boesch, C; Trcek, J; Sievers, M; Teuber, M

    1998-03-01

    Strains of a new species in the genus Acetobacter, for which we propose the name A. intermedius sp. nov., were isolated and characterized in pure culture from different sources (Kombucha beverage, cider vinegar, spirit vinegar) and different countries (Switzerland, Slovenia). The isolated strains grow in media with 3% acetic acid and 3% ethanol as does A. europaeus, do, however, not require acetic acid for growth. These characteristics phenotypically position A. intermedius between A. europaeus and A. xylinus, DNA-DNA hybridizations of A. intermedius-DNA with DNA of the type strains of Acetobacter europaeus, A. xylinus, A. aceti, A. hansenii, A. liquefaciens, A. methanolicus, A. pasteurianus, A. diazotrophicus, Gluconobacter oxydans and Escherichia coli HB 101 indicated less than 60% DNA similarity. The important features of the new species are described. Acetobacter intermedius strain TF2 (DSM11804) isolated from the liquid phase of a tea fungus beverage (Kombucha) is the type strain. PMID:13678040

  14. SAFETY PROCEDURE SP-24 NATIONAL HIGH MAGNETIC

    E-print Network

    Weston, Ken

    SAFETY PROCEDURE SP-24 NATIONAL HIGH MAGNETIC FIELD LABORATORY NHMFL FLORIDA STATE UNIVERSITY SAFETY PROCEDURE SP-24 VISITOR AND CONTRACTOR SAFETY DIRECTOR, ENVIRONMENTAL, HEALTH, SAFETY & SECURITY Angela Sutton

  15. SP-100 space reactor safety

    SciTech Connect

    Not Available

    1987-05-01

    The SP-100 space reactor power system is being developed to meet the large electrical power requirements of civilian and military missions planned for the 1990's and beyond. It will remove the restrictions on electrical power generation that have tended to limit missions and will enable the fuller exploration and utilization of space. This booklet describes the SP-100 space reactor power system and its development. Particular emphasis is given to safety. The design aand operational features as well as the design and safety review process that will assure that the SP-100 can be launched nd operated safely are described.

  16. MPI Programming Environment for IBM SP1\\/SP2

    Microsoft Academic Search

    Hubertus Franke; Ching-farn Eric Wu; Michel Riviere; Pratap Pattnaik; Marc Snir

    1995-01-01

    In this paper we discuss an implementation of the MessagePassing Interface standard (MPI) for the IBMScalable Power PARALLEL 1 and 2 (SP1, SP2). Keyto a reliable and efficient implementation of a messagepassing library on these machines is the careful designof a UNIX-Socket like layer in the user space with controlledaccess to the communication adapters and withadequate recovery and flow control.

  17. Nonomuraea rosea sp. nov.

    PubMed

    Kämpfer, Peter; Busse, Hans-Jürgen; Tindall, Brian J; Nimtz, Manfred; Grün-Wollny, Iris

    2010-05-01

    A Gram-positively staining, aerobic, non-motile actinomycete, strain GW 12687(T), that formed rose-pigmented colonies and branched substrate and aerial mycelia was studied in detail for its taxonomic position. On the basis of 16S rRNA gene sequence similarity studies, strain GW 12687(T) was grouped into the genus Nonomuraea, being most closely related to Nonomuraea dietziae (97.6 %), Nonomuraea africana (97.1 %), and Nonomuraea kuesteri (97.1 %). The 16S rRNA gene sequence similarity to other species of the genus Nonomuraea was < or =97 %. The chemotaxonomic characterization supported allocation of the strain to the genus Nonomuraea. The major menaquinone was MK-9(H(4)) with minor amounts of MK-9(H(2)), MK-9(H(6)), MK-9(H(0)) and MK-8(H(4)). The polar lipid profile contained the major compound diphosphatidylglycerol, moderate amounts of phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, and an unknown aminophosphoglycolipid. Phosphatidylinositol mannosides and phosphatidylinositol were also present. The major fatty acids were iso- and anteiso- and 10-methyl-branched fatty acids. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW 12687(T) from closely related species. Thus, GW 12687(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea rosea sp. nov. is proposed, with GW 12687(T) (=DSM 45177(T) =CCUG 56107(T)) as the type strain. PMID:19666797

  18. Nonomuraea kuesteri sp. nov.

    PubMed

    Kämpfer, Peter; Kroppenstedt, Reiner M; Grün-Wollny, Iris

    2005-03-01

    A Gram-positive, aerobic, non-motile actinomycete, strain GW 14-1925(T), that formed branched substrate and aerial mycelium was studied using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strain GW 14-1925(T) was shown to belong to the genus Nonomuraea, being most closely related to Nonomuraea longicatena (97.9 %), Nonomuraea turkmeniaca (98.9 %), Nonomuraea helvata (98.6 %), Nonomuraea polychroma (98.5 %), Nonomuraea salmonaea (98.3 %), Nonomuraea roseoviolacea subsp. roseoviolacea (98.1 %) and Nonomuraea roseoviolacea subsp. carminata (97.7 %). The 16S rRNA gene sequence similarity to other Nonomuraea species was <97.5 %. Chemotaxonomic data [major menaquinones of the MK-9 series with minor amounts of MK-8(H(4)); major polar lipids of phospholipid type IV; fatty acids with major amounts of iso- and anteiso- and 10-methyl-branched fatty acids in combination with iso-branched 2-hydroxy fatty acids] supported allocation of the strain to the genus Nonomuraea. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW 14-1925(T) from closely related species; thus, GW 14-1925(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea kuesteri sp. nov. is proposed, with GW 14-1925(T) (=DSM 44753(T)=NRRL B-24325(T)) as the type strain. PMID:15774673

  19. Nonomuraea dietzii sp. nov..

    PubMed

    Stackebrandt, E; Wink, J; Steiner, U; Kroppenstedt, R M

    2001-07-01

    The taxon 'Sebekia benihana', an actinomycete of industrial significance, has been subjected to a polyphasic taxonomic study. 16S rDNA sequence analyses of strain DSM 44320 (= NRRL 11111) revealed 100% similarity to the 16S rDNA sequence of Nonomuraea roseola DSM 43767T and high values (>98.5%) to some other species of this genus. DNA-DNA similarity values of less than 31% observed between strain DSM 44320 and N. roseola DSM 43767T, Nonomuraea recticatena DSM 43937T and Nonomuraea africana DSM 43748T indicate that this strain represents an individual genospecies. Differences between strain DSM 44320T and the 16 validly described Nonomuraea species occur in the primary structure of the 16S rDNA, the physiological test profile, the morphology of mycelium and the spore surface and the colour of substrate mycelium. The only strain of 'S. benihana', DSM 44320T (= NRRL 11111T), is proposed as the type strain of a new species of the genus Nonomuraea, Nonomuraea dietzii sp. nov.. PMID:11491343

  20. SP-100 Advanced Technology Program

    NASA Technical Reports Server (NTRS)

    Sovie, Ronald J.

    1987-01-01

    The goal of the triagency SP-100 Program is to develop long-lived, compact, lightweight, survivable nuclear reactor space power systems for application to the power range 50 kWe to 1 MWe. The successful development of these systems should enable or significantly enhance many of the future NASA civil and commercial missions. The NASA SP-100 Advanced Technology Program strongly augments the parallel SP-100 Ground Engineering System Development program and enhances the chances for success of the overall SP-100 program. The purpose of this paper is to discuss the key technical elements of the Advanced Technology Program and the progress made in the initial year and a half of the project.

  1. SP-100 advanced technology program

    SciTech Connect

    Sovie, R.J.

    1987-01-01

    The goal of the triagency SP-100 Program is to develop long-lived, compact, lightweight, survivable nuclear reactor space power systems for application to the power range 50 kWe to 1 MWe. The successful development of these systems should enable or significantly enhance many of the future NASA civil and commercial missions. The NASA SP-100 Advanced Technology Program strongly augments the parallel SP-100 Ground Engineering System Development program and enhances the chances for success of the overall SP-100 program. The purpose of this paper is to discuss the key technical elements of the Advanced Technology Program and the progress made in the initial year and a half of the project.

  2. Pseudomonas grimontii sp. nov.

    PubMed

    Baïda, Nader; Yazourh, Asmae; Singer, Elisabeth; Izard, Daniel

    2002-09-01

    The vernacular name 'fluorescent Pseudomonas group 97-514' was coined for a group of 43 strains isolated from two French natural mineral waters. All these strains were gram-negative, rod-shaped and motile by means of a single polar flagellum. They produced fluorescent pigment (pyoverdin) on King B medium, catalase and cytochrome oxidase. They were capable of respiratory but not fermentative metabolism. They were not able to accumulate poly-beta-hydroxybutyrate and possessed an arginine dihydrolase system. DNA-DNA relatedness studies (S1 nuclease method) showed that the 43 strains of 'fluorescent Pseudomonas group 97-514' formed a genetically homogeneous group (DNA-DNA relatedness ranged from 70 to 100%). A total of 76 strains representing well-known or partially characterized species of the genus Pseudomonas sensu stricto had 7-56% DNA hybridization with strain CFML 97-514T. The highest DNA binding values were found with Pseudomonas veronii CIP 104663T (52%), Pseudomonas rhodesiae CIP 104664T (56%), Pseudomonas marginalis ATCC 10844T (56%), Pseudomonas gessardii CIP 105469T (53%) and Pseudomonas cedrella CIP 105541T (52%). Their unrelatedness was confirmed by deltaTm values greater than 7 degrees C. On the basis of the results of phenotypic and DNA-DNA hybridization studies, a novel Pseudomonas species, Pseudomonas grimontii sp. nov., is proposed for the 43 strains of 'fluorescent Pseudomonas group 97-514'. The type strain is strain CFML 97-514T (= CIP 106645T = ATCC BAA-140T). The G+C content of the DNA of the type strain was 58 mol%. A comparison of the complete 16S rRNA gene sequence of the type strain CFML 97-514T and the sequence of other strains of the genus Pseudomonas revealed that the novel species fell within the 'Pseudomonas fluorescens intrageneric cluster'. Members of P. grimontii grew at 4 degrees C but not at 41 degrees C. They were able to use D-xylose, alpha-L-rhamnose, alpha-aminobutyrate, meso-erythritol and itaconate as sole sources of carbon and energy and formed levan from sucrose. Strains do not possess lecithinase or Tween esterase activities. The clinical significance of P. grimontii is unknown. PMID:12361251

  3. [Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil].

    PubMed

    Guimarães, Antônio Marcos; Alves, Endrigo Gabellini Leonel; de Rezende, Glycia Ferreira; Rodrigues, Marcelo Costa

    2005-04-01

    Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both Baermann's method and centrifugal flotation technique. Toxocara sp. and Ancylostoma sp. eggs were observed in soil samples collected from public squares in 17.4% (4/23) and 69.6 (16/23) respectively. In schools and child day care settings the contamination by Ancylostoma sp. larva in sand samples was 11.1% (2/18). Public parks are settings of more potential risk of Toxocara sp. eggs and Ancylostoma sp. infection. Stool parasitology testing of 174 stool samples showed 58% and 23% of Ancylostoma sp and Toxocara sp eggs infection respectively. PMID:15895151

  4. NASA SP-4214 HASGONE BEFORE

    E-print Network

    Rathbun, Julie A.

    . Series: NASA SP ; 4214. TL789.8.U6A528 1989 919.9'104 88-600242 For sale by the Superintendent is only partly to record the engineering and scientific accomplishments of the men and women who made sent men and women to the moon, no American robot, how- ever sophisticated or important, would produce

  5. SP-100 control system modeling

    NASA Technical Reports Server (NTRS)

    Meyer, R. A.; Halfen, F. J.; Alley, A. D.

    1987-01-01

    SP-100 Control Systems modeling was done using a thermal hydraulic transient analysis model called ARIES-S. The ARIES-S Computer Simulation provides a basis for design, integration and analysis of the reactor including the control and protection systems. It is a modular digital computer simulation written in FORTRAN that operates interactively in real time on a VAX minicomputer.

  6. The Sp(1)-Kepler problems

    SciTech Connect

    Meng Guowu [Department of Mathematics, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon (Hong Kong)

    2009-07-15

    Let n{>=}2 be a positive integer. To each irreducible representation {sigma} of Sp(1), an Sp(1)-Kepler problem in dimension (4n-3) is constructed and analyzed. This system is superintegrable, and when n=2 it is equivalent to a generalized MICZ-Kepler problem in dimension of 5. The dynamical symmetry group of this system is O-tilde*(4n) with the Hilbert space of bound states H({sigma}) being the unitary highest weight representation of O*-tilde(4n) with highest weight, (-1,{center_dot}{center_dot}{center_dot},-1,-(1+{sigma})), which occurs at the rightmost nontrivial reduction point in the Enright-Howe-Wallach classification diagram for the unitary highest weight modules. Here {sigma} is the highest weight of {sigma}. Furthermore, it is shown that the correspondence {sigma}{r_reversible}H({sigma}) is the theta-correspondence for dual pair (Sp(1),O*(4n))subset Sp(8n,R)

  7. Argonne's SpEC Module

    SciTech Connect

    Harper, Jason

    2014-05-05

    Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

  8. Sporulation of Cyclospora sp. oocysts.

    PubMed Central

    Smith, H V; Paton, C A; Mitambo, M M; Girdwood, R W

    1997-01-01

    Cyclospora sp. oocysts sporulated maximally at 22 and 30 degrees C for 14 days retarded sporulation. Up to 12% of human- and baboon-derived oocysts previously stored at 4 degrees C for 1 to 2 months sporulated when stored for 6 to 7 days at 30 degrees C. PMID:9097458

  9. Scaling study for SP100 reactor technology

    Microsoft Academic Search

    A. C. Marshall; B. McKissock; OH Cleveland

    1989-01-01

    Several ways were explored of extending SP-100 reactor technology to higher power levels. One approach was to use the reference SP-100 pin design and increase the fuel pin length and the number of fuel pins as needed to provide higher capability. The impact on scaling of a modified and advanced SP-100 reactor technology was also explored. Finally, the effect of

  10. Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter.

    PubMed

    Houbraken, Jos; López-Quintero, Carlos A; Frisvad, Jens C; Boekhout, Teun; Theelen, Bart; Franco-Molano, Ana Esperanza; Samson, Robert A

    2011-06-01

    Several species of the genus Penicillium were isolated during a survey of the mycobiota of leaf litter and soil in Colombian Amazon forest. Five species, Penicillium penarojense sp. nov. (type strain CBS 113178(T)?=?IBT 23262(T)), Penicillium wotroi sp. nov. (type strain CBS 118171(T)?=?IBT 23253(T)), Penicillium araracuarense sp. nov. (type strain CBS 113149(T)?=?IBT 23247(T)), Penicillium elleniae sp. nov. (type strain CBS 118135(T)?=?IBT 23229(T)) and Penicillium vanderhammenii sp. nov. (type strain CBS 126216(T)?=?IBT 23203(T)) are described here as novel species. Their taxonomic novelty was determined using a polyphasic approach, combining phenotypic, molecular (ITS and partial ?-tubulin sequences) and extrolite data. Phylogenetic analyses showed that each novel species formed a unique clade for both loci analysed and that they were most closely related to Penicillium simplicissimum, Penicillium janthinellum, Penicillium daleae and Penicillium brasilianum. An overview of the phylogeny of this taxonomically difficult group is presented, and 33 species are accepted. Each of the five novel species had a unique extrolite profile of known and uncharacterized metabolites and various compounds, such as penicillic acid, andrastin A, pulvilloric acid, paxillin, paspaline and janthitrem, were commonly produced by these phylogenetically related species. The novel species had a high growth rate on agar media, but could be distinguished from each other by several macro- and microscopical characteristics. PMID:20639229

  11. Increased SP4 and SP1 transcription factor expression in the postmortem hippocampus of chronic schizophrenia.

    PubMed

    Pinacho, Raquel; Valdizán, Elsa M; Pilar-Cuellar, Fuencisla; Prades, Roger; Tarragó, Teresa; Haro, Josep Maria; Ferrer, Isidre; Ramos, Belén

    2014-11-01

    Altered levels of transcription factor specificity protein 4 (SP4) and 1 (SP1) in the cerebellum, prefrontal cortex and/or lymphocytes have been reported in severe psychiatric disorders, including early psychosis, bipolar disorder, and chronic schizophrenia subjects who have undergone long-term antipsychotic treatments. SP4 transgenic mice show altered hippocampal-dependent psychotic-like behaviours and altered development of hippocampal dentate gyrus. Moreover, NMDAR activity regulates SP4 function. The aim of this study was to investigate SP4 and SP1 expression levels in the hippocampus in schizophrenia, and the possible effect of antipsychotics and NMDAR blockade on SP protein levels in rodent hippocampus. We analysed SP4 and SP1 expression levels in the postmortem hippocampus of chronic schizophrenia (n = 14) and control (n = 11) subjects by immunoblot and quantitative RT-PCR. We tested the effect of NMDAR blockade on SP factors in the hippocampus of mouse treated with an acute dose of MK801. We also investigated the effect of subacute treatments with haloperidol and clozapine on SP protein levels in the rat hippocampus. We report that SP4 protein and both SP4 and SP1 mRNA expression levels are significantly increased in the hippocampus in chronic schizophrenia. Likewise, acute treatment with MK801 increased both SP4 and SP1 protein levels in mouse hippocampus. In contrast, subacute treatment with haloperidol and clozapine did not significantly alter SP protein levels in rat hippocampus. These results suggest that SP4 and SP1 upregulation may be part of the mechanisms deregulated downstream of glutamate signalling pathways in schizophrenia and might be contributing to the hippocampal-dependent cognitive deficits of the disorder. PMID:25175639

  12. Pseudonocardia cypriaca sp. nov., Pseudonocardia salamisensis sp. nov., Pseudonocardia hierapolitana sp. nov. and Pseudonocardia kujensis sp. nov., isolated from soil.

    PubMed

    Sahin, Nevzat; Veyisoglu, Aysel; Tatar, Demet; Spröer, Cathrin; Cetin, Demet; Guven, Kiymet; Klenk, Hans-Peter

    2014-05-01

    The taxonomic positions of four novel actinomycetes isolated from soil samples, designated KT2142T, PM2084T, K236T and A4038T, were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus Pseudonocardia. Whole-cell hydrolysates of the four strains contained meso-diaminopimelic acid and arabinose and galactose as the diagnostic sugars (cell-wall type IV). Their predominant menaquinone was found to be MK-8(H4). The major fatty acid was iso-C16:0. 16S rRNA gene sequence data supported the classification of the isolates in the genus Pseudonocardia and showed that they formed four distinct branches within the genus. DNA-DNA relatedness studies between the isolates and their phylogenetic neighbours showed that they belonged to distinct genomic species. The four isolates were readily distinguished from one another and from the type strains of species classified in the genus Pseudonocardia based on a combination of phenotypic and genotypic properties. In conclusion, it is proposed that the four isolates be classified in four novel species of the genus Pseudonocardia, for which the names Pseudonocardia cypriaca sp. nov. (type strain KT2142T=KCTC 29067T=DSM 45511T=NRRL B-24882T), Pseudonocardia hierapolitana sp. nov. (type strain PM2084T=KCTC 29068T=DSM 45671T=NRRL B-24879T), Pseudonocardia salamisensis sp. nov. (type strain K236T=KCTC 29100T=DSM 45717T) and Pseudonocardia kujensis sp. nov. (type strain A4038T=KCTC 29062T=DSM 45670T=NRRL B-24890T) are proposed. PMID:24523445

  13. Transcription factor Sp3 antagonizes activation of the ornithine decarboxylase promoter by Sp1.

    PubMed Central

    Kumar, A P; Butler, A P

    1997-01-01

    Ornithine decarboxylase (ODC) expression is important for proliferation and is elevated in many tumor cells. We previously showed that Sp1 is a major positive regulator of ODC transcription. In this paper we have investigated transcriptional regulation of rat ODC by the closely related factor Sp3. While over-expression of Sp1 caused a dramatic activation of the ODC promoter, over-expression of Sp3 caused little or no activation in either Drosophila SL2 cells (lacking endogenous Sp1 or Sp3) or in H35 rat hepatoma cells. Furthermore, co-transfection studies demonstrated that Sp3 abolished trans -activation of the ODC promoter by Sp1. DNase I footprint studies and electrophoretic mobility shift assays demonstrated that both recombinant Sp1 and Sp3 bind specifically to several sites within the ODC promoter also protected by nuclear extracts, including overlapping GC and CT motifs located between -116 and -104. This CT element is a site of negative ODC regulation. Mutation of either element reduced binding, but mutation of both sites was required to eliminate binding of either Sp1 or Sp3. These results demonstrate that ODC is positively regulated by Sp1 and negatively regulated by Sp3, suggesting that the ratio of these transcription factors may be an important determinant of ODC expression during development or transformation. PMID:9115370

  14. Listeria floridensis sp. nov., Listeria aquatica sp. nov., Listeria cornellensis sp. nov., Listeria riparia sp. nov. and Listeria grandensis sp. nov., from agricultural and natural environments.

    PubMed

    den Bakker, Henk C; Warchocki, Steven; Wright, Emily M; Allred, Adam F; Ahlstrom, Christina; Manuel, Clyde S; Stasiewicz, Matthew J; Burrell, Angela; Roof, Sherry; Strawn, Laura K; Fortes, Esther; Nightingale, Kendra K; Kephart, Daniel; Wiedmann, Martin

    2014-06-01

    Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average BLAST nucleotide identity (ANIb) of less than 85% to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria; (i) a clade representing Listeria monocytogenes, L. marthii, L. innocua, L. welshimeri, L. seeligeri and L. ivanovii, which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188(T)?=?DSM 26686(T)?=?LMG 28120(T)?=?BEI NR-42633(T)) and Listeria floridensis sp. nov. (type strain FSL S10-1187(T)?=?DSM 26687(T)?=?LMG 28121(T)?=?BEI NR-42632(T)), (iii) a clade consisting of Listeria rocourtiae, L. weihenstephanensis and three novel species, Listeria cornellensis sp. nov. (type strain TTU A1-0210(T)?=?FSL F6-0969(T)?=?DSM 26689(T)?=?LMG 28123(T)?=?BEI NR-42630(T)), Listeria grandensis sp. nov. (type strain TTU A1-0212(T)?=?FSL F6-0971(T)?=?DSM 26688(T)?=?LMG 28122(T)?=?BEI NR-42631(T)) and Listeria riparia sp. nov. (type strain FSL S10-1204(T)?=?DSM 26685(T)?=?LMG 28119(T)?=?BEI NR- 42634(T)) and (iv) a clade containing Listeria grayi. Genomic and phenotypic data suggest that the novel species are non-pathogenic. PMID:24599893

  15. Rhodium(I)-Catalyzed Sequential C(sp)?C(sp(3) ) and C(sp(3) )?C(sp(3) ) Bond Formation through Migratory Carbene Insertion.

    PubMed

    Xia, Ying; Feng, Sheng; Liu, Zhen; Zhang, Yan; Wang, Jianbo

    2015-06-26

    A Rh(I) -catalyzed three-component reaction of tert-propargyl alcohol, diazoester, and alkyl halide has been developed. This reaction can be considered as a carbene-involving sequential alkyl and alkynyl coupling, in which C(sp)?C(sp(3) ) and C(sp(3) )?C(sp(3) ) bonds are built successively on the carbenic carbon atom. The Rh(I) -carbene migratory insertion of an alkynyl moiety and subsequent alkylation are proposed to account for the two separate C?C bond formations. This reaction provides an efficient and tunable method for the construction of all-carbon quaternary center. PMID:25982105

  16. MPI on IBM SP1\\/SP2: current status and future directions

    Microsoft Academic Search

    Hubertus Franke; Peter Hochschild; P. Pattnik J. Prost; Jean-Pierre Prost; Marc Snir

    1994-01-01

    A complete prototype implementation of MPI on the IBM Scalable Power PARALLEL 1 and 2 (SP1, SP2) is discussed. This implementation achieves essentially the same performance as the native EUI library, although MPI is much larger. The paper describes the implementation of EUI on SP1\\/SP2, and the modifications required to to implement MPI, initial performance measurements, and directions for future

  17. Nonomuraea bangladeshensis sp. nov. and Nonomuraea coxensis sp. nov.

    PubMed

    Ara, Ismet; Kudo, Takuji; Matsumoto, Atsuko; Takahashi, Yoko; Omura, Satoshi

    2007-07-01

    Two novel bacterial strains were isolated from sandy soil from Cox's Bazar, Bangladesh. Strains 5-10-10(T) and 5-38-42(T) were Gram-positive, aerobic, non-motile actinomycetes that form branched substrate and aerial mycelium. On the basis of 16S rRNA gene sequence similarity studies, the novel strains were shown to belong to the genus Nonomuraea, being most closely related to Nonomuraea fastidiosa. Chemotaxonomic data supported the assignment of the novel strains as members of the genus Nonomuraea. Strain 5-10-10(T) contained MK-9(H(4)) and strain 5-38-42(T) contained MK-9(H(6)) and MK-9(H(4)) as the major menaquinones. Major polar lipids were phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The major cellular fatty acid for strain 5-10-10(T) was iso-C(16 : 0) (26.4 %); C(16 : 0) (17.4 %) was the major cellular fatty acid for strain 5-38-42(T). The results of DNA-DNA hybridization and physiological tests enabled strains 5-10-10(T) and 5-38-42(T) to be differentiated genotypically and phenotypically from each other and from the closely related species, N. fastidiosa. On the basis of these results, strains 5-10-10(T) and 5-38-42(T) represent two novel species of the genus Nonomuraea. Following an evaluation of morphological, physiological and chemotaxonomic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization experiments, the new isolates are proposed as two novel species, Nonomuraea bangladeshensis sp. nov. [type strain, 5-10-10(T) (=MTCC 8089(T)=JCM 13930(T))] and Nonomuraea coxensis sp. nov. [type strain, 5-38-42(T) (=MTCC 8090(T)=JCM 13931(T))]. PMID:17625184

  18. Precursor forms of substance P (SP) in nervous tissue: detection with antisera to SP, SP-Gly, and SP-Gly-Lys

    SciTech Connect

    Kream, R.M.; Schoenfeld, T.A.; Mancuso, R.; Clancy, A.N.; El-Bermani, W.; Macrides, F.

    1985-07-01

    Antisera generated to substance P-Gly (SP-G) and substance P-Gly-Lys (SP-G-K), the likely unamidated COOH-terminally extended forms of substance P, were used to quantify and localize substance P precursor forms in hamster brain stem and spinal cord. The precursor determinant SP-G-K was liberated from larger heterogeneous forms by mild trypsinization of tissue extracts and was converted into the second precursor determinant, SP-G, by subsequent treatment with carboxypeptidase B. The basal levels of SP-G-K in brain stem and spinal cord were approx. = 0.5 pg/mg of tissue and rose 43- to 64-fold after trypsinization. Basal levels of Sp-G were comparable to those of SP-G-K and rose 10- to 29-fold after combined enzyme treatments. Immunohistochemical labeling of axons and somata with anti-SP-G-K increased dramatically after trypsinization. Collectively, these results support the hypothesis that substance P is synthesized from larger precursors and demonstrate that extended precursor forms are normally present in the axons and somata of neural systems that synthesize substance P.

  19. _q .. SP-6102 -" IN SYSTEMS ENGINEERING

    E-print Network

    Rhoads, James

    _¢q .. SP-6102 -" READINGS IN SYSTEMS ENGINEERING Edited by Francis T. Hoban and William M. Lawbaugh co ! (NASA-SP-6102) REAOINGS IN SYSTEMS ENGINEERING (NASa) 215 p N93-24678 --THRU-- N93-24693 Unclas H1/31 0158570 #12;.J T ,j J #12;READINGS IN SYSTEMS ENGINEERING Edited by Francis T. Hoban

  20. Canonical Representations sp (2n, R)

    SciTech Connect

    Rangarajan, G.

    1991-08-01

    In this paper, we study a rather unconventional real basis for the real symplectic algebra sp(2n,R). We demonstrate the utility of this basis for practical computations by giving a simple derivation of the second and fourth order indices of irreducible representations of sp(2n,R).

  1. ORNITHOCHEYLETIA MIRONOVI SP.N. (ACARI: CHEYLETIDAE) — ????? ??? ?????????????? ?????? ? ??? ?????? ???????? ?? ????????

    Microsoft Academic Search

    A. V. Bochkov

    A new species of parasitic mites, Ornithochey- ^~^ mironovi sp.n. (Acari: Cheyletidae) is described from bank swallow Riparia riparia (L.) (Passeri- formes: Hirundinidae) from Kirghizia. ?????? ?????? ????? ??? ?????????????? ?????? Ornithocheyletia mironovi sp.n. (Acari: Cheyletidae) ? ????????? ???????? Riparia riparia (L.) (Passer- iformes: Hirundinidae) ?? ????????. Two species of the genus OmithocheyletiaVolgm, 1964 were known from the territory of

  2. Glyphosate catabolism by Pseudomonas sp

    SciTech Connect

    Shinabarger, D.L.

    1986-01-01

    The pathway for the degradation of glyphosate (N-phosphonomethylglycine) by Pseudomonas sp. PG2982 has been determined using metabolic radiolabeling experiments. Radiorespirometry experiments utilizing (3-/sup 14/C) glyphosate revealed that approximately 50-59% of the C3 carbon was oxidized to CO/sub 2/. Fractionation of stationary phase cells labeled with (3-/sup 14/C)glyphosate revealed that from 45-47% of the assimilated C3 carbon is distributed to proteins and that amino acids methionine and serine are highly labeled. The nucleic acid bases adenine and guanine received 90% of the C3 label that was incorporated into nucleic acids, and the only pyrimidine base labeled was thymine. Pulse labeling of PG2982 cells with (3-/sup 14/C)glyphosate revealed that (3-/sup 14/C)sarcosine is an intermediate in glyphosate degradation. Examination of crude extracts prepared from PG2982 cells revealed the presence of an enzyme that oxidizes sarcosine to glycine and formaldehyde. These results indicate that the first step in glyphosate degradation by PG2982 is cleavage of the carbon-phosphorus bond, resulting in the release of sarcosine and a phosphate group. The phosphate group is utilized as a source of phosphorus, and the sarcosine is degraded to glycine and formaldehyde. Phosphonate utilization by Pseudomonas sp. PG2982 was investigated. Each of the ten phosphonates tested were utilized as a sole source of phosphorus by PG2982. Representative compounds tested included alkylphosphonates, 1-amino-substituted alkylphosphonates, amino-terminal phosphonates, and an arylphosphonate. PG2982 cultures degraded phenylphosphonate to benzene and produced methane from methylphosphonate. The data indicate that PG2982 is capable of cleaving the carbon-phosphorus bond of several structurally different phosphonates.

  3. Differences in nutrient uptake capacity of the benthic filamentous algae Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. under varying N/P conditions.

    PubMed

    Liu, Junzhuo; Vyverman, Wim

    2015-03-01

    The N/P ratio of wastewater can vary greatly and directly affect algal growth and nutrient removal process. Three benthic filamentous algae species Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. were isolated from a periphyton bioreactor and cultured under laboratory conditions on varying N/P ratios to determine their ability to remove nitrate and phosphorus. The N/P ratio significantly influenced the algal growth and phosphorus uptake process. Appropriate N/P ratios for nitrogen and phosphorus removal were 5-15, 7-10 and 7-20 for Cladophora sp., Klebsormidium sp. and Pseudanabaena sp., respectively. Within these respective ranges, Cladophora sp. had the highest biomass production, while Pseudanabaena sp. had the highest nitrogen and phosphorus contents. This study indicated that Cladophora sp. had a high capacity of removing phosphorus from wastewaters of low N/P ratio, and Pseudanabaena sp. was highly suitable for removing nitrogen from wastewaters with high N/P ratio. PMID:25544498

  4. Differences in the alveolar macrophage proteome in transgenic mice expressing human SP-A1 and SP-A2

    PubMed Central

    Phelps, David S.; Umstead, Todd M.; Silveyra, Patricia; Hu, Sanmei; Wang, Guirong; Floros, Joanna

    2014-01-01

    Surfactant protein A (SP-A) plays a number of roles in lung host defense and innate immunity. There are two human genes, SFTPA1 and SFTPA2, and evidence indicates that the function of SP-A1 and SP-A2 proteins differ in several respects. To investigate the impact of SP-A1 and SP-A2 on the alveolar macrophage (AM) phenotype, we generated humanized transgenic (hTG) mice on the SP-A knockout (KO) background, each expressing human SP-A1 or SP-A2. Using two-dimensional difference gel electrophoresis (2D-DIGE) we studied the AM cellular proteome. We compared mouse lines expressing high levels of SPA1, high levels of SP-A2, low levels of SP-A1, and low levels of SP-A2, with wild type (WT) and SP-A KO mice. AM from mice expressing high levels of SP-A2 were the most similar to WT mice, particularly for proteins related to actin and the cytoskeleton, as well as proteins regulated by Nrf2. The expression patterns from mouse lines expressing higher levels of the transgenes were almost the inverse of one another – the most highly expressed proteins in SP-A2 exhibited the lowest levels in the SP-A1 mice and vice versa. The mouse lines where each expressed low levels of SP-A1 or SP-A2 transgene had very similar protein expression patterns suggesting that responses to low levels of SP-A are independent of SP-A genotype, whereas the responses to higher amounts of SP-A are genotype-dependent. Together these observations indicate that in vivo exposure to SP-A1 or SP-A2 differentially affects the proteomic expression of AMs, with SP-A2 being more similar to WT. PMID:24729982

  5. Differences in the alveolar macrophage proteome in transgenic mice expressing human SP-A1 and SP-A2.

    PubMed

    Phelps, David S; Umstead, Todd M; Silveyra, Patricia; Hu, Sanmei; Wang, Guirong; Floros, Joanna

    2013-04-01

    Surfactant protein A (SP-A) plays a number of roles in lung host defense and innate immunity. There are two human genes, SFTPA1 and SFTPA2, and evidence indicates that the function of SP-A1 and SP-A2 proteins differ in several respects. To investigate the impact of SP-A1 and SP-A2 on the alveolar macrophage (AM) phenotype, we generated humanized transgenic (hTG) mice on the SP-A knockout (KO) background, each expressing human SP-A1 or SP-A2. Using two-dimensional difference gel electrophoresis (2D-DIGE) we studied the AM cellular proteome. We compared mouse lines expressing high levels of SPA1, high levels of SP-A2, low levels of SP-A1, and low levels of SP-A2, with wild type (WT) and SP-A KO mice. AM from mice expressing high levels of SP-A2 were the most similar to WT mice, particularly for proteins related to actin and the cytoskeleton, as well as proteins regulated by Nrf2. The expression patterns from mouse lines expressing higher levels of the transgenes were almost the inverse of one another - the most highly expressed proteins in SP-A2 exhibited the lowest levels in the SP-A1 mice and vice versa. The mouse lines where each expressed low levels of SP-A1 or SP-A2 transgene had very similar protein expression patterns suggesting that responses to low levels of SP-A are independent of SP-A genotype, whereas the responses to higher amounts of SP-A are genotype-dependent. Together these observations indicate that in vivo exposure to SP-A1 or SP-A2 differentially affects the proteomic expression of AMs, with SP-A2 being more similar to WT. PMID:24729982

  6. Launch vehicle integration requirements for SP-100

    SciTech Connect

    Shaw, L.T. Jr.; Womack, J.R.

    1984-01-31

    SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS) Space Shuttle System into a near-earth orbit, deployed, and through upper stage propulsion burn(s) be inserted/transferred to its mission orbit. The nature of the advanced design SP-100 gives rise to a set of issues that require special attention to assure that payloads using this power plant are physically and functionally compatible with the NSTS and meet the safety requirements thereof. The purpose of this document is to define and present the requirements and interface provisions that, when satisfied, will ensure technical compability between SP-100 systems and the NSTS.

  7. SP100 THERMOELECTRIC CELL TESTING AT JPL

    Microsoft Academic Search

    Richard Ewell; Andrew Zoltan; BRAZE FOIL

    1994-01-01

    Three prototypic SP-100 thermoelectric cells, fabricated by Martin Marietta Astro Space in Valley Forge, Pennsylvania, were tested in vacuum at prototypic temperatures at JPL. Their thermal and electrical performance were characterized with 200\\

  8. Heterorhabditis marelatusn. sp. (Rhabditida: Heterorhabditidae) from Oregon

    Microsoft Academic Search

    Jie Liu; Ralph E Berry

    1996-01-01

    A new species of the nematode genusHeterorhabditiswas found during a survey of soil entomopathogenic nematodes near Seaside, Oregon.Heterorhabditis marelatusn. sp. differs from other species ofHeterorhabditisby the length of infective juvenile and gubernaculum, and the shape of spicules, bursa, and genital papillae.Heterorhabditis marelatusn. sp. has distinct random amplified polymorphic DNA (RAPD) fragments, with a minimal RAPD polymorphism frequency of 0.85 to

  9. Sp1/Sp3 involved in activation of GATA-1 Cell Research | Vol 18 No 2 | February 2008

    E-print Network

    Tian, Weidong

    Sp1/Sp3 involved in activation of GATA-1 302 npg Cell Research | Vol 18 No 2 | February 2008 ORIGINAL ARTICLE Involvement of Sp1/Sp3 in the activation of the GATA-1 erythroid promoter in K562 cells August 2007; published online 15 January 2008 GATA-1 is a hematopoietic transcription factor

  10. Neophyllobius lorestanicus sp. nov. and N. ostovani sp.nov. (Acari: Camerobiidae) from Iran.

    PubMed

    Khanjani, Mohammad; Hoseini, Mohammad Ahmad; Yazdanpanah, Shima; Masoudian, Farshad

    2014-01-01

    Two new species of the genus Neophyllobius Berlese, 1886 are described: Neophyllobius lorestanicus sp. nov. collected from soil under Prunus domestica L. (Rosaceae) in Markazi province and Neophyllobius ostovani sp. nov. from soil and rotten leaves of oak trees in Fars province, Iran. A key to all known Iranian and Turkish species of Neophyllobius is provided. PMID:24870646

  11. Theoretical investigations of sp-sp2 hybridized zero-dimensional fullerenynes

    NASA Astrophysics Data System (ADS)

    Zhang, Shengli; Zhang, Yonghong; Huang, Shiping; Wang, Chunru

    2012-04-01

    Widely recognized as the quintessential material, sp2 hybridized carbon material with low dimensions, such as zero-dimensional fullerene, one-dimensional carbon nanotube and two-dimensional graphene, has already compiled an impressive list of superlatives. Quite recently, one-dimensional sp-sp2 hybridized carbon tubular arrays with a wall thickness of about 40 nm and two-dimensional carbon films with the average thickness of 970 nm have been synthesized successfully. Thus, we expect that the existence of a sp-sp2 hybridized zero-dimensional carbon allotrope is possible. A novel and stable zero-dimensional carbon allotrope (fullerenyne) with sp-sp2 hybridization is introduced by means of density functional theory calculation and molecular dynamics confirmation. Unique porous characteristic C96 fullernenyne with an Oh symmetry group exhibits exceptionally high stability. We hope that the present study will lead to a further development of a broad new class of carbon materials.Widely recognized as the quintessential material, sp2 hybridized carbon material with low dimensions, such as zero-dimensional fullerene, one-dimensional carbon nanotube and two-dimensional graphene, has already compiled an impressive list of superlatives. Quite recently, one-dimensional sp-sp2 hybridized carbon tubular arrays with a wall thickness of about 40 nm and two-dimensional carbon films with the average thickness of 970 nm have been synthesized successfully. Thus, we expect that the existence of a sp-sp2 hybridized zero-dimensional carbon allotrope is possible. A novel and stable zero-dimensional carbon allotrope (fullerenyne) with sp-sp2 hybridization is introduced by means of density functional theory calculation and molecular dynamics confirmation. Unique porous characteristic C96 fullernenyne with an Oh symmetry group exhibits exceptionally high stability. We hope that the present study will lead to a further development of a broad new class of carbon materials. Electronic Supplementary Information (ESI) available: Description of theoretical methods for calculating fullerenynes, and all fullerenyne models from C24 to C120 with different symmetry groups and the corresponding energy gaps.

  12. Assessment of Surfactant Protein A (SP-A) dependent agglutination

    PubMed Central

    2010-01-01

    Background Monomers of the collectin surfactant associated protein-A (SP-A) are arranged in trimers and higher oligomers. The state of oligomerization differs between individuals and likely affects SP-A's functional properties. SP-A can form aggregates together with other SP-A molecules. Here we report and assess a test system for the aggregate forming properties of SP-A in serum and broncho-alveolar lavage samples. Methods Anti-SP-A antibodies fixed to latex beads bound SP-A at its N-terminal end and allowed the interaction with other SP-A molecules in a given sample by their C-terminal carbohydrate recognition domain (CRD) to agglutinate the beads to aggregates, which were quantified by light microscopy. Results SP-A aggregation was dependent on its concentration, the presence of calcium, and was dose-dependently inhibited by mannose. Unaffected by the presence of SP-D no aggregation was observed in absence of SP-A. The more complex the oligomeric structure of SP-A present in a particular sample, the better was its capability to induce aggregation at a given total concentration of SP-A. SP-A in serum agglutinated independently of the pulmonary disease; in contrast SP-A in lung lavage fluid was clearly inferior in patients with chronic bronchitis and particularly with cystic fibrosis compared to controls. Conclusions The functional status of SP-A with respect to its aggregating properties in serum and lavage samples can be easily assessed. SP-A in lung lavage fluid in patients with severe neutrophilic bronchitis was inferior. PMID:21092225

  13. Hepatozoon sp. in wild carnivores in Texas.

    PubMed

    Mercer, S H; Jones, L P; Rappole, J H; Twedt, D; Lack, L L; Craig, T M

    1988-07-01

    Twelve coyotes (Canis latrans), three bobcats (Lynx rufus) and six ocelots (Felis pardalis) from the Gulf Coast of Texas were infected with Hepatozoon sp. The geographic distribution of infected wild animals coincides with the highest prevalence of Hepatozoon canis infection in domestic dogs for which the wild species may act as a reservoir. PMID:3411720

  14. Scaling study for SP-100 reactor technology

    NASA Astrophysics Data System (ADS)

    Marshall, A. C.; McKissock, B.

    Several ways were explored of extending SP-100 reactor technology to higher power levels. One approach was to use the reference SP-100 pin design and increase the fuel pin length and the number of fuel pins as needed to provide higher capability. The impact on scaling of a modified and advanced SP-100 reactor technology was also explored. Finally, the effect of using alternative power conversion subsystems, with SP-100 reactor technology was investigated. One of the principal concerns for any space based system is mass; consequently, this study focused on estimating reactor, shield, and total system mass. The RSMASS code (Marshall 1986) was used to estimate reactor and shield mass. Simple algorithms developed at NASA-Lewis were used to estimate the balance of system mass. Power ranges from 100 kWe to 10 MWe were explored assuming both one year and seven years of operation. Thermoelectric, Stirling, Rankine, and Brayton power conversion systems were investigated. The impact on safety, reliability, and other system attributes, caused by extending the technology to higher power levels, was also investigated.

  15. SP100 radiator design trade study

    Microsoft Academic Search

    Richard Ewell

    1992-01-01

    A design trade study of the SP-100 heat rejection subsystem (HRSS) was made. A system code was used to evaluate the sensitivity of the HRSS mass and performance to changes. Variations in heat pipe diameter and cross-section, fin length and thickness, armor thickness, and overall configuration and materials were evaluated. The analysis indicates that the minimum system mass occurs for

  16. New anamorphic yeast species: Candida infanticola sp. nov., Candida polysorbophila sp. nov., Candida transvaalensis sp. nov., and Trigonopsis californica sp. nov.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three new species of Candida and a new species of Trigonopsis are described based on their recognition from phylogenetic analysis of gene sequences from large subunit ribosomal RNA, ITS1/ITS2 rRNA, mitochondrial small subunit rRNA and cytochrome oxidase II. Candida infanticola sp. nov. (type strain...

  17. Power transmission studies for tethered SP-100

    NASA Technical Reports Server (NTRS)

    Bents, David J.

    1988-01-01

    The tether and/or transmission line connecting the SP-100 to space station presents some unorthodox challenges in high voltage engineering, power transmission, and distribution. The line, which doubles as a structural element of this unusual spacecraft, will convey HVDC from SP-100 to the platform in low Earth orbit, and environment where the local plasma is sufficient to cause breakdown of exposed conductors at potentials of only a few hundred volts. Its anticipated several years operation, and continuously accumulating exposure to meteoroids and debris, raises an increasing likelihood that mechanical damage, including perforation, will be sustained in service. The present concept employs an array of gas insulated solid wall aluminum coaxial tubes; a conceptual design which showed basic feasibility of the SP-100 powered space station. Practical considerations of launch, deployment and assembly have lead to investigation of reel deployable, dielectric insulated coaxial cables. To be competitive, the dielectric would have to operate reliably in a radiation environment under electrical stresses exceeding 50 kV/cm. The SP-100 transmission line high voltage interfaces are also considered.

  18. Power transmission studies for tethered SP-100

    NASA Technical Reports Server (NTRS)

    Bents, David J.

    1988-01-01

    The tether and/or transmission line connecting the SP-100 to Space Station presents some unorthodox challenges in high voltage engineering, power transmission, and distribution. The line, which doubles as a structural element of this unusual spacecraft, will convey HVDC from SP-100 to the platform in low Earth orbit, and environment where the local plasma is sufficient to cause breakdown of exposed conductors at potentials of only a few hundred volts. Its anticipated several years operation, and continuously accumulating exposure to meteoroids and debris, raises an increasing likelihood that mechanical damage, including perforation, will be sustained in service. The present concept employs an array of gas insulated solid wall aluminum coaxial tubes; a conceptual design which showed basic feasibility of the SP-100 powered Space Station. Practical considerations of launch, deployment and assembly have led to investigation of reel deployable, dielectric insulated coaxial cables. To be competitive, the dielectric would have to operate reliably in a radiation environment under electrical stresses exceeding 50 kV/cm. The SP-100 transmission line high voltage interfaces are also considered.

  19. Uranium accumulation by Pseudomonas sp. EPS5028

    Microsoft Academic Search

    Ana M. Marqués; Xavier Roca; M. Dolores Simon-Pujol; M. Carmen Fuste; Francisco Congregado

    1991-01-01

    Pseudomonas sp. EPS-5028 was examined for the ability to accumulate uranium from solutions. The uptake of uranium by this microorganism is very rapid and is affected by pH but not by temperature, metabolic inhibitors, culture time and the presence of various cations and anions. The amount of uranium absorbed by the cells increased as the uranium concentration of the solution

  20. SP100 inhibits ETS1 activity in primary endothelial cells

    Microsoft Academic Search

    John S Yordy; Omar Moussa; Huiping Pei; Damien Chaussabel; Runzhao Li; Dennis K Watson

    2005-01-01

    SP100 was first identified as a nuclear autoimmune antigen and is a constituent of the nuclear body. SP100 interacts with the ETS1 transcription factor, and we have previously shown that SP100 reduces ETS1-DNA binding and inhibits ETS1 transcriptional activity on the MMP1 and uPA promoters. We now demonstrate that SP100 expression is upregulated by interferons, which have been shown to

  1. Effect of Hydrophobic Surfactant Proteins SP-B and SP-C on Binary Phospholipid Monolayers

    PubMed Central

    Brockman, Jennifer M.; Wang, Zhengdong; Notter, Robert H.; Dluhy, Richard A.

    2003-01-01

    In situ external reflection infrared spectroscopy at the air-water interface was used to study the influence on phospholipid structure of an endogenous mixture of the two hydrophobic surfactant proteins, SP-B and SP-C, which are thought to play pivotal roles in the adsorption and function of pulmonary surfactant. Mixtures studied were 1:1, 2:1, and 7:1 (mol:mol) DPPC-d62:DPPG, and 7:1 DPPC-d62:DOPG, alone and in the presence of 0.5–10 wt % mixed SP-B/C purified chromatographically from calf lung surfactant extract. Perdeuteration of DPPC produced a shift in vibrational frequencies so that it could be differentiated spectroscopically from the phosphoglycerol component in the surface monolayer. CH2 antisymmetric and symmetric stretching bands (?2920 and 2852 cm?1) along with the analogous CD2 stretching bands (?2194 and 2089 cm?1) were analyzed, and band heights and peak wavenumber positions were assessed as a function of monolayer surface pressure. Small, near-physiological contents of 1–2 wt % SP-B/C typically produced the maximum observed spectroscopic effects, which were abolished at high protein contents of 10 wt %. Analysis of CH2 and CD2 stretching bands and C-H/C-D band height ratios indicated that SP-B/C affected PC and PG lipids differently within the surface monolayer. SP-B/C had preferential interactions with DPPG in 1:1, 2:1, and 7:1 DPPC-d62:DPPG films that increased its acyl chain order. SP-B/C also interacted specifically with DOPG in 7:1 DPPC-d62:DOPG monolayers, but in this case an increase in CH2 band heights and peak wavenumber positions indicated a further disordering of the already fluid DOPG acyl chains. CD2 band height and peak wavenumber analysis indicated that SP-B/C had no significant effect on the structure of DPPC-d62 chains in 7:1 films with DPPG or DOPG, and had only a slight tendency to increase the acyl chain order in 1:1 films of DPPC-d62:DPPG. SP-B/C had no significant effect on DPPC-d62 structure in films with DOPG. Infrared results also indicated that interactions involving SP-B/C and lipids led to exclusion of PC and PG lipids from the compressed interfacial monolayer, in agreement with our previous report on the phase morphology of lipid monolayers containing 1 wt % SP-B/C. PMID:12524286

  2. O Glycosylation of an Sp1Derived Peptide Blocks Known Sp1 Protein Interactions

    Microsoft Academic Search

    MARK D. ROOS; KAIHONG SU; JOHN R. BAKER; JEFFREY E. KUDLOW

    1997-01-01

    The O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins is dynamic and abundant in the nucleus and cytosol. Several transcription factors, including Sp1, have been shown to contain this modification; however, the functional role of O-GlcNAc in these proteins has not been determined. In this paper we describe the use of the previously characterized glutamine-rich transactivation domain of Sp1 (B-c) as a

  3. Colonization and growth promotion characteristics of Enterobacter sp. and Herbaspirillum sp. on Brassica oleracea

    Microsoft Academic Search

    Muhammad Zakria; Asuka Ohsako; Yuichi Saeki; Akihiro Yamamoto; Shoichiro Akao

    2008-01-01

    We investigated the effects of inoculating a dicot plant, Brassica oleracea, with nitrogen-fixing endophytic bacteria isolated from monocots. The bacteria used were Enterobacter sp. strain 35 isolated from sugarcane and Herbaspirillum sp. strain B501 isolated from wild rice. Under glasshouse conditions, B. oleracea inoculated with strain 35 had a significantly greater fresh weight than uninoculated plants, and the fresh weight of

  4. SP100i Syringe Pump WORLD PRECISION INSTRUMENTS 15

    E-print Network

    Kleinfeld, David

    SP100i Syringe Pump WORLD PRECISION INSTRUMENTS 15 INSTRUCTION MANUAL Serial No._____________________ 8/94 World Precision Instruments, Inc. SP100i Syringe Pump Digital Infusion Syringe Pump #12;SP100i Syringe Pump WORLD PRECISION INSTRUMENTS 1 Contents GENERAL DESCRIPTION

  5. Thaumatomyrmex soesilae sp. nov. from Suriname ((Hymenoptera: Formicidae)

    E-print Network

    Villemant, Claire

    Thaumatomyrmex soesilae sp. nov. from Suriname ((Hymenoptera: Formicidae) by Dr Dewanand Makhan sp. nov. from Suriname (Hymenoptera: Formicidae). Calodema Supplementary Paper No. 58:1-3. Abstract: A new species of Thaumatomyrmex, T. soesilae sp. nov. (Hymenoptera: Formicidae) is described from

  6. 76 FR 22694 - SP 49 Pipeline LLC; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-22

    ...ENERGY Federal Energy Regulatory Commission [Docket No. AC11-56-000] SP 49 Pipeline LLC; Notice of Filing Take notice that on April 12, 2011, SP 49 Pipeline LLC (``SP 49'') submitted a request for waiver of the requirement to file...

  7. Chapter 6.25 Sp3 Model 655 Series

    E-print Network

    Healy, Kevin Edward

    Purpose 1.1 The Sp3 model 655 series hot filament CVD (HFCVD) diamond deposition reactor produces thin film polycrystalline diamond coatings on 4" and 6" Si wafers. The hot filament technology allowsChapter 6.25 Sp3 Model 655 Series HFCVD Diamond Deposition Reactor (sp3) (595) 1.0 Equipment

  8. Remote sensing data of SP Mountain and SP Lava flow in North-Central Arizona

    USGS Publications Warehouse

    Schaber, G.G.; Elachi, C.; Farr, T.G.

    1980-01-01

    Multifrequency airborne radar image data of SP Mountain [Official name of feature (U.S. Geological Survey, 1970)] and SP flow (and vicinity) in north-central Arizona were obtained in diverse viewing directions and direct and cross-polarization, then compared with surface and aerial photography, LANDSAT multispectral scanner data, airborne thermal infrared imagery, surface geology, and surface roughness statistics. The extremely blocky, basaltic andesite of SP flow is significantly brighter on direct-polarization K-band (0.9-cm wavelength) images than on cross-polarized images taken simultaneously. Conversely, for the longer wavelength (25 cm) L-band radar images, the cross-polarization image returns from SP flow are brighter than the direct-polarized image. This effect is explained by multiple scattering and the strong wavelength dependence of polarization effects caused by the rectilinear basaltic andesite scatters. Two distinct types of surface relief on SP flow, one extremely blocky, the other subdued, are found to be clearly discriminated on the visible and thermal wavelength images but are separable only on the longer wavelength L-band radar image data. The inability of the K- and X- (3-cm wavelength) band radars to portray the differences in roughness between the two SP flow surface units is attributed to the radar frequency dependence of the surface-relief scale, which, described as the Rayleigh criterion, represents the transition between quasispecular and primarily diffuse backscatter. ?? 1980.

  9. Pulmonary surfactant protein A (SP-A) specifically binds dipalmitoylphosphatidylcholine

    SciTech Connect

    Kuroki, Y.; Akino, T. (Sapporo Medical College (Japan))

    1991-02-15

    Phospholipids are the major components of pulmonary surfactant. Dipalmitoylphosphatidylcholine is believed to be especially essential for the surfactant function of reducing the surface tension at the air-liquid interface. Surfactant protein A (SP-A) with a reduced denatured molecular mass of 26-38 kDa, characterized by a collagen-like structure and N-linked glycosylation, interacts strongly with a mixture of surfactant-like phospholipids. In the present study the direct binding of SP-A to phospholipids on a thin layer chromatogram was visualized using 125I-SP-A as a probe, so that the phospholipid specificities of SP-A binding and the structural requirements of SP-A and phospholipids for the binding could be examined. Although 125I-SP-A bound phosphatidylcholine and sphingomyeline, it was especially strong in binding dipalmitoylphosphatidylcholine, but failed to bind phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, and phosphatidylserine. Labeled SP-A also exhibited strong binding to distearoylphosphatidylcholine, but weak binding to dimyristoyl-, 1-palmitoyl-2-linoleoyl-, and dilinoleoylphosphatidylcholine. Unlabeled SP-A readily competed with labeled SP-A for phospholipid binding. SP-A strongly bound dipalmitoylglycerol produced by phospholipase C treatment of dipalmitoylphosphatidylcholine, but not palmitic acid. This protein also failed to bind lysophosphatidylcholine produced by phospholipase A2 treatment of dipalmitoylphosphatidylcholine. 125I-SP-A shows almost no binding to dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylethanolamine. The addition of 10 mM EGTA into the binding buffer reduced much of the 125I-SP-A binding to phospholipids. Excess deglycosylated SP-A competed with labeled SP-A for binding to dipalmitoylphosphatidylcholine, but the excess collagenase-resistant fragment of SP-A failed.

  10. The osmoprotective effect of some organic solutes on Streptomyces sp. mado2 and nocardiopsis sp. mado3 growth

    PubMed Central

    Ameur, Hanane; Ghoul, Mostefa; Selvin, Joseph

    2011-01-01

    The response of two marine actinomycetes such as Streptomyces sp. MADO2 and Nocardiopsis sp. MADO3 to osmotic stress in minimal medium M63 and in glycerol-asparagine medium (ISP5) was studied. The two strains were moderately halophilic and the behavior of the strain Streptomyces sp. MADO2 and Nocardiopsis sp. MADO3 towards the salt stress was varied depends on the media composition and the salinity concentration. The strain Streptomyces sp. was more sensitive to salt stress than Nocardiopsis sp. The growth of both Streptomyces sp. and Nocardiopsis sp. were inhibited at 1 M NaCl irrespective of the medium used. The Nocardiopsis sp. acquired osmoadaptation on ISP5 medium whereas the Streptomyces sp. showed poor growth on M63 medium. Glycine betaine (GB), proline and trehalose played a critical role in osmotic adaptation at high osmolarity whereas at low osmolarity they showed an inhibitory effect on the bacterial growth. The present findings confirmed that GB was the powerful osmoprotectant for Streptomyces sp. and Nocardiopsis sp. grown at 1 M NaCl both in M63 and ISP5 media. PMID:24031666

  11. Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1

    SciTech Connect

    Ito, Tatsuo [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan)] [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Kitamura, Haruka; Uwatoko, Chisana; Azumano, Makiko [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan)] [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan); Itoh, Kohji, E-mail: kitoh@ph.tokushima-u.ac.jp [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan)] [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Kuwahara, Jun, E-mail: jkuwahar@dwc.doshisha.ac.jp [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan)] [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan)

    2010-12-10

    Research highlights: {yields} Sp1 zinc fingers themselves interact with importin {alpha}. {yields} Sp1 zinc finger domains play an essential role as a nuclear localization signal. {yields} Sp1 can be transported into the nucleus in an importin-dependent manner. -- Abstract: Transcription factor Sp1 is localized in the nucleus and regulates the expression of many cellular genes, but the nuclear transport mechanism of Sp1 is not well understood. In this study, we revealed that GST-fused Sp1 protein bound to endogenous importin {alpha} in HeLa cells via the Sp1 zinc finger domains, which comprise the DNA binding domain of Sp1. It was found that the Sp1 zinc finger domains directly interacted with a wide range of importin {alpha} including the armadillo (arm) repeat domain and the C-terminal acidic domain. Furthermore, it turned out that all three zinc fingers of Sp1 are essential for binding to importin {alpha}. Taken together, these results suggest that the Sp1 zinc finger domains play an essential role as a NLS and Sp1 can be transported into the nucleus in an importin-dependent manner even though it possesses no classical NLSs.

  12. Effects of temperature on methanogenesis in a thermophilic (58°C) anaerobic digestor. [Methanosarcina sp. ; Methanothrix sp. ; Methanobacterium sp

    Microsoft Academic Search

    S. H. Zinder; T. Anguish; S. C. Cardwell

    1984-01-01

    The short-term effects of temperature on methanogenesis from acetate or COâ in a thermophilic (58°C) anaerobic digestor were studied by incubating digestor sludge at different temperatures with ¹⁴C-labeled methane precursors (¹⁴CHâCOO⁻ or ¹⁴COâ). During a period when Methanosarcina sp. was numerous in the sludge, methanogenesis from acetate was optimal at 55 to 60°C and was completely inhibited at 65°C. A

  13. Effects of temperature on methanogenesis in a thermophilic (58/sup 0/C) anaerobic digestor. [Methanosarcina sp. ; Methanothrix sp. ; Methanobacterium sp

    SciTech Connect

    Zinder, S.H.; Anguish, T.; Cardwell, S.C.

    1984-04-01

    The short-term effects of temperature on methanogenesis from acetate or CO/sub 2/ in a thermophilic (58/sup 0/C) anaerobic digestor were studied by incubating digestor sludge at different temperatures with /sup 14/C-labeled methane precursors (/sup 14/CH/sub 3/COO/sup -/ or /sup 14/CO/sub 2/). During a period when Methanosarcina sp. was numerous in the sludge, methanogenesis from acetate was optimal at 55 to 60/sup 0/C and was completely inhibited at 65/sup 0/C. A Methanosarcina culture isolated from the digestor grew optimally on acetate at 55 to 58/sup 0/C and did not grow or produce methane at 65/sup 0/C. An accidental shift of digestor temperature from 58 to 64/sup 0/C during this period caused a sharp decrease in gas production and a large increase in acetate concentration within 24 h, indicating that the aceticlastic methanogens in the digestor were the population most susceptible to this temperature increase. During a later period when Methanothrix sp. was numerous in the digestor, methanogenesis from /sup 14/CH/sub 3/COO/sup -/ was optimal at 65/sup 0/C and completely inhibited at 75/sup 0/C. Methanogenesis from /sup 14/CO/sub 2/ in the sludge was optimal at 65/sup 0/C and still proceeded at 75/sup 0/C. A CO/sub 2/-reducing methanobacterium sp. isolated from the digestor was capable of methanogenesis at 75/sup 0/C. During the period when Methanothix sp. was apparently dominant, sludge incubated for 24 h at 65/sup 0/C produced more methane than sludge incubated at 60/sup 0/C, and no acetate accumulated at 65/sup 0/C. Methanogenesis was severely inhibited in sludge incubated at 70/sup 0/C, but since neither acetate nor H/sub 2/ accumulated, production of these methanogenic substrates by fermentative bacteria was probably the most temperature-sensitive process. 19 references.

  14. Ogataea kolombanensis sp. nov., Ogataea histrianica sp. nov. and Ogataea deakii sp. nov., three novel yeast species from plant sources.

    PubMed

    ?adež, Neža; Dlauchy, Dénes; Raspor, Peter; Péter, Gábor

    2013-08-01

    Nine methanol-assimilating yeast strains isolated from olive oil sediments in Slovenia, extra virgin olive oil from Italy and rotten wood collected in Hungary were found to form three genetically separated groups, distinct from the currently recognized yeast species. Sequence analysis from genes of the small subunit (SSU) rRNA, internal transcribed spacer region/5.8S rRNA, large subunit (LSU) rRNA D1/D2 domains and translational elongation factor-1? (EF-1?) revealed that the three closely related groups represent three different undescribed yeast species. Sequence analysis of the LSU rRNA gene D1/D2 domains placed the novel species in the Ogataea clade. The three novel species are designated as Ogataea kolombanensis sp. nov. (type strain: ZIM 2322(T) = CBS 12778(T) = NRRL Y-63657(T)), Ogataea histrianica sp. nov. (type strain: ZIM 2463(T) = CBS 12779(T) = NRRL Y-63658(T)) and Ogataea deakii sp. nov. (type strain: NCAIM Y.01896(T) = CBS 12735(T) = NRRL Y-63656(T)). PMID:23749284

  15. Significant biological role of Sp1 transactivation in multiple myeloma

    PubMed Central

    Fulciniti, Mariateresa; Amin, Samir; Nanjappa, Puru; Rodig, Scott; Prabhala, Rao; Li, Cheng; Minvielle, Stephane; Tai, Yu-tzu; Tassone, Pierfrancesco; Avet-Loiseau, Herve; Hideshima, Teru; Anderson, Kenneth C.; Munshi, Nikhil C.

    2015-01-01

    Purpose The transcription factor Sp1 controls number of cellular processes by regulating the expression of critical cell cycle, differentiation and apoptosis-related genes containing proximal GC/GT-rich promoter elements. We here provide both experimental and clinical evidence that Sp1 plays an important regulatory role in MM cell growth and survival. Experimental design We have investigated the functional Sp1 activity in MM cells using a plasmid with renilla luciferase reporter gene driven by Sp1-responsive promoter. We have also used both SiRNA and ShRNA-mediated Sp1 knock-down to investigate the growth and survival effects of Sp1 on MM cells, and further investigated the anti-MM activity of Terameprocol (TMP), a small molecule which specifically competes with Sp1-DNA binding in vitro and in vivo. Results We have confirmed high Sp1 activity in MM cells which is further induced by adhesion to bone marrow stromal cells (BMSC). Sp1 knock down decreases MM cell proliferation and induces apoptosis. Sp1-DNA binding inhibition by TMP inhibits MM cell growth both in vitro and in vivo, inducing caspase 9-dependent apoptosis and overcoming the protective effects of BMSCs. Conclusions Our results demonstrate Sp1 as an important transcription factor in myeloma that can be therapeutically targeted for clinical application by TMP. PMID:21856768

  16. Jaborandi (Pilocarpus sp., rutaceae): A wild species

    Microsoft Academic Search

    CLAUDIO URBANO B. PINHEIRO; Claudio Urbano; B. Pinheiro

    1997-01-01

    Jaborandi (Pilocarpus sp.;) is a shrub or small tree 3-7.5 m tall that can be found in native stands or as isolated individuals throughout Brazil.\\u000a The leaves of jaborandi contain the alkaloid pilocarpine, widely used in ophthalmology for treatment of certain types of glaucoma\\u000a and more recently for xerostomia. This paper discusses the current systems of production of jaborandi leaves

  17. Pectin lyase from Aspergillus sp. CHY1043

    Microsoft Academic Search

    Luis Delgado; Blanca A. Trejo; Carlos Huitrón; Guillermo Aguilar

    1993-01-01

    Aspergillus sp. CH-Y-1043 synthesizes pectin lyase when grown on citrus pectin at 37° C. Production is favoured by increased esterification degree of the pectin used as carbon source. This enzyme displays higher activity at pH values of 8.5–8.8 and temperatures of 40–45° C. The optimal substrate for the enzyme was highly esterified pectin and no enzymatic activity was registered on

  18. One-dimensional s-p superlattice

    E-print Network

    Wojciech Ganczarek; Michele Modugno; Giulio Pettini; Jakub Zakrzewski

    2014-06-23

    The physics of one dimensional optical superlattices with resonant $s$-$p$ orbitals is reexamined in the language of appropriate Wannier functions. It is shown that details of the tight binding model realized in different optical potentials crucially depend on the proper determination of Wannier functions. We discuss the properties of a superlattice model which quasi resonantly couples $s$ and $p$ orbitals and show its relation with different tight binding models used in other works.

  19. Microbial flocculant from Arcuadendron sp. TS49

    Microsoft Academic Search

    Soon Ho Lee; Sang Ok Lee; Kyung Lib Jang; Tae Ho Lee

    1995-01-01

    A flocculant was purified from the culture broth of Archuadendron sp. TS-49 by a series of precipitations with acetone, 60% ammonium sulfate-butanol, salting-out by dialysis, and cetylpyridinium chloride. The flocculating activity was observed most highly at pH 3.0 and markedly enhanced by the addition of salts, especially in the case of FeCl3 or FeSO4. This bioflocculant efficiently flocculated all tested

  20. Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp.

    PubMed

    Silva, R F; Lupatini, M; Trindade, L; Antoniolli, Z I; Steffen, R B; Andreazza, R

    2013-01-01

    Environments contaminated with heavy metals negatively impact the living organisms. Ectomycorrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L(-1) in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L(-1) in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated for 30 days at 28 °C. UFSC-Pt116 showed high copper-resistance such as accessed by CL50 determinations (concentration to reduce 50% of the growth) as while as UFSC-PT24 displayed copper-resistance mechanism at 0.50 mmol L(-1) in solid medium. The UFSC-PT24 and UFSC-Sc124 isolates have increased copper-resistance in liquid medium. The higher production of extracellular pigment was detected in UFSC-Pt116 cultures. The UFSC-Pt116 and UFSC-PT24 isolates showed higher resistance for copper and produced higher mycelium biomass than the other isolates. In this way, the isolates UFSG-Pt116 and UFSC-PT24 can be important candidates to survive in copper-contaminated areas, and can show important role in plants symbiosis in these contaminated sites. PMID:24294261

  1. Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp.

    PubMed Central

    Silva, R.F.; Lupatini, M.; Trindade, L.; Antoniolli, Z.I.; Steffen, R.B.; Andreazza, R

    2013-01-01

    Environments contaminated with heavy metals negatively impact the living organisms. Ectomycorrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L?1 in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L?1 in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated for 30 days at 28 °C. UFSC-Pt116 showed high copper-resistance such as accessed by CL50 determinations (concentration to reduce 50% of the growth) as while as UFSC-PT24 displayed copper-resistance mechanism at 0.50 mmol L?1 in solid medium. The UFSC-PT24 and UFSC-Sc124 isolates have increased copper-resistance in liquid medium. The higher production of extracellular pigment was detected in UFSC-Pt116 cultures. The UFSC-Pt116 and UFSC-PT24 isolates showed higher resistance for copper and produced higher mycelium biomass than the other isolates. In this way, the isolates UFSG-Pt116 and UFSC-PT24 can be important candidates to survive in copper-contaminated areas, and can show important role in plants symbiosis in these contaminated sites. PMID:24294261

  2. Enhanced Endoglucanase Production by Soil Isolates of Fusarium sp. and Aspergillus sp. through Submerged Fermentation Process (Topraktan Batik Kültür Fermentasyonu ile ?zole Edilen Fusarium sp. ve

    Microsoft Academic Search

    Paulchamy Chellapandi; Abha Apurvabhai Jani

    Objective: The objective was to optimize the fermentation media components and condi- tions to improve the production yield of endoglucanase by filamentous fungi isolated from garden soil. Methods: Cellulolytic fungi were screened from garden soil and identified as Fusarium sp. and Aspergillus sp. by using conidial morphology. The influences of various culture condi- tions including incubation time, temperature, pH, carbon,

  3. Early RNAs in SP82- and SP01-infected Bacillus subtilis may be processed.

    PubMed Central

    Downard, J S; Whiteley, H R

    1981-01-01

    Transcription of SP82 and SP01 DNAs in vitro by Bacillus subtilis RNA polymerase yielded mostly large RNA species, with many in excess of 1,500 bases in length, whereas most of the RNAs synthesized in vivo early in infection were much smaller. Addition of an extract from uninfected B. subtilis to reaction mixtures containing RNAs synthesized in vitro generated additional discrete RNAs whose mobilities on polyacrylamide gels matched the mobilities of some of the smaller RNAs synthesized in vivo. Images PMID:6785448

  4. Biosystems Engineering Catalog 2013 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU

    E-print Network

    Tennessee, University of

    Biosystems Engineering Catalog 2013 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 28 or Math SAT 630 EF 105

  5. Biosystems Engineering Catalog 2014 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU

    E-print Network

    Grissino-Mayer, Henri D.

    Biosystems Engineering Catalog 2014 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 28 or Math SAT 630 EF 105

  6. Mechanical Engineering Catalog 2014 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU

    E-print Network

    Grissino-Mayer, Henri D.

    Mechanical Engineering Catalog 2014 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 28 or Math SAT 630 EF 105

  7. Biomedical Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA,SP,SU Chem 120 or 128 (4) FA, SP, SU

    E-print Network

    Grissino-Mayer, Henri D.

    Biomedical Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA,SP,SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT 28 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 or Math SAT 630 EF 105

  8. Aerospace Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU

    E-print Network

    Grissino-Mayer, Henri D.

    Aerospace Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT 28 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 or Math SAT 630 EF 105

  9. Nuclear Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP

    E-print Network

    Grissino-Mayer, Henri D.

    Nuclear Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP 16 hours Prereq- Math 130 or math ACT 28 Math 130 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 or Math SAT 630 EF 105

  10. Specificity proteins Sp1 and Sp3 interact with the rat GTP Cyclohydrolase I proximal promoter to regulate transcription

    PubMed Central

    Chandran, Nitya Sarath; Vunnava, Prashanthi; Wu, Yanning; Kapatos, Gregory

    2008-01-01

    The role of the proximal promoter GC-box in regulating basal and cAMP-dependent GTP Cyclohydrolase I gene transcription was investigated using a variety of cell lines and techniques. These studies show that the GC-box is composed of a triad of cis-elements that in vitro bind specificity proteins Sp1 and Sp3. Sp1 and Sp3 were found associated with the native proximal promoter in PC12 cells but were not recruited to the promoter during cAMP-dependent transcription. Studies using Drosophila SL2 cells showed that Sp3 occupies two sites within the GC-box and enhances transcription when acting alone and synergistically when combined with nuclear factor-Y (NF-Y) and CCAAT/Enhancer-Binding Protein (C/EBP)?, cognate binding proteins for the adjacent cAMP response element (CRE) and CCAAT-box cAMP response elements. In contrast, Sp1 bound only one site within the GC-box and did not enhance transcription unless combined with NF-Y and C/EBP?. Studies in SL2 cells also showed that Sp1 and Sp3 do not co-occupy the GC-box, and accordingly Sp1 competes for Sp3 binding to repress Sp3-dependent transcription. In PC12 cells, complete mutation of the GC-box reduced basal but not cAMP-dependent transcription, resulting in an overall increase in the cAMP response and demonstrating that formation of this enhanceosome does not require Sp1 or Sp3. Experiments in which the GC-box was replaced with a Gal4 element and the promoter challenged with Gal4 fusion proteins support this conclusion and a role for Sp3 in maintaining high levels of basal transcription in PC12 cells. Equivalent amounts of Sp1 and Sp3 were found associated with the native proximal promoter in PC12 and Rat2 cells, which differ 10-fold in basal transcription. Similar levels of methylation of CpG dinucleotides located within the GC-box were also observed in these two cells lines. These results suggest that Sp1 and Sp3 bound to the GC-box might help to preserve an open chromatin configuration at the proximal promoter in cells which constitutively express low levels of GTP Cyclohydrolase I. PMID:18004997

  11. Early SP-100 flight mission designs

    NASA Astrophysics Data System (ADS)

    Josloff, Allan T.; Shepard, Neal F.; Kirpich, Aaron S.; Murata, Ronald; Smith, Michael A.; Stephen, James D.

    1993-01-01

    Early flight mission objectives can be met with a Space Reactor Power System (SRPS) using thermoelectric conversion in conjunction with fast spectrum, lithium-cooled reactors. This paper describes two system design options using thermoelectric technology to accommodate an early launch. In the first of these options, radiatively coupled Radioiosotope Thermoelectric Generator (RTG) unicouples are adapted for use with a SP-100-type reactor heat source. Unicouples have been widely used as the conversion technology in RTGs and have demonstrated the long-life characteristics necessary for a highly relible SRPS. The thermoelectric leg height is optimized in conjunction with the heat rejection temperature to provide a mass optimum 6-kWe system configured for launch on a Delta II launch vehicle. The flight-demonstrated status of this conversion technology provides a high confidence that such a system can be designed, assembled, tested, and launched by 1997. The use of a SP-100-type reactor assures compliance with safety requirements and expedites the flight safety approval process while, at the same time, providing flight performance verification for a heat source technology with the growth potential to meet future national needs for higher power levels. A 15-kW2, Atlas IIAS-launched system using the compact, conductively coupled multicouple converters being developed under the SP-100 program to support an early flight system launch also described. Both design concepts have been scaled to 20-kWe in order to support recent studies by DOE/NASA for higher power early launch missions.

  12. CoSP: a general framework for computational soundness proofs

    Microsoft Academic Search

    Michael Backes; Dennis Hofheinz; Dominique Unruh

    2009-01-01

    We describe CoSP, a general framework for conducting computational soundness proofs of symbolic models and for embedding these proofs into formal calculi. CoSP considers arbitrary equational theories and computational implementations, and it abstracts away many details that are not crucial for proving computational soundness, such as message scheduling, corruption models, and even the internal structure of a protocol. CoSP enables

  13. Granulicella paludicola gen. nov., sp. nov., Granulicella pectinivorans sp. nov., Granulicella aggregans sp. nov. and Granulicella rosea sp. nov., acidophilic, polymer-degrading acidobacteria from Sphagnum peat bogs.

    PubMed

    Pankratov, Timofey A; Dedysh, Svetlana N

    2010-12-01

    Five strains of strictly aerobic, heterotrophic bacteria that form pink-red colonies and are capable of hydrolysing pectin, xylan, laminarin, lichenan and starch were isolated from acidic Sphagnum peat bogs and were designated OB1010(T), LCBR1, TPB6011(T), TPB6028(T) and TPO1014(T). Cells of these isolates were Gram-negative, non-motile rods that produced an amorphous extracellular polysaccharide-like substance. Old cultures contained spherical bodies of varying sizes, which represent starvation forms. Cells of all five strains were acidophilic and psychrotolerant, capable of growth at pH 3.0-7.5 (optimum pH 3.8-4.5) and at 2-33°C (optimum 15-22°C). The major fatty acids were iso-C(15?:?0), C(16?:?0) and summed feature 3 (C(16?:?1)?7c and/or iso-C(15?:?0) 2-OH). The major menaquinone detected was MK-8. The pigments were carotenoids. The genomic DNA G+C contents were 57.3-59.3 mol%. The five isolates were found to be members of subdivision 1 of the phylum Acidobacteria and displayed 95.3-98.9?% 16S rRNA gene sequence similarity to each other. The closest described relatives to strains OB1010(T), LCBR1, TPB6011(T), TPB6028(T), and TPO1014(T) were members of the genera Terriglobus (94.6-95.8?% 16S rRNA gene sequence similarity) and Edaphobacter (94.2-95.4?%). Based on differences in cell morphology, phenotypic characteristics and hydrolytic capabilities, we propose a novel genus, Granulicella gen. nov., containing four novel species, Granulicella paludicola sp. nov. with type strain OB1010(T) (=DSM 22464(T) =LMG 25275(T)) and strain LCBR1, Granulicella pectinivorans sp. nov. with type strain TPB6011(T) (=VKM B-2509(T) =DSM 21001(T)), Granulicella rosea sp. nov. with type strain TPO1014(T) (=DSM 18704(T) =ATCC BAA-1396(T)) and Granulicella aggregans sp. nov. with type strain TPB6028(T) (=LMG 25274(T) =VKM B-2571(T)). PMID:20118293

  14. SP-100, a project manager's view

    NASA Technical Reports Server (NTRS)

    Truscello, Vincent C.

    1983-01-01

    Born to meet the special needs of America's space effort, the SP-100 Program testifies to the cooperation among government agencies. The Department of Energy (DOE), the National Aeronautics and Space Administration (NASA), and the Defense Advanced Research Projects Agency (DARPA) are working together to produce a 100-kW power system for use in outer space. At this point in the effort, it is appropriate to review: The approach to meet program goals; the status of activities of the Project Office, managed by the Jet Propulsion Laboratory (JPL); and, because this is a meeting on materials, answers beings developed by the Project Office to vital questions on refractory alloy technology.

  15. Binary NS simulations using SpEC

    NASA Astrophysics Data System (ADS)

    Haas, Roland; Szilagyi, Bela; Kaplan, Jeffrey; Ott, Christian; Lippuner, Jonas; Scheel, Mark; Barkett, Kevin; Muhlberger, Curran; Foucart, Francois; Duez, Matthew

    2014-03-01

    NSNS binaries are expected to be one of the major sources of gravitational radiation detectable by Advanced LIGO. Together with neutrinos, gravitational waves are our only means to learn about the processes deep within a merging pair of NS, shedding light on the as yet poorly understood, equation of state governing matter at nuclear densities and beyond. We report on binary neutron star simulations using the Spectral Einstein Code (SpEC) developed by the Caltech-Cornell-CITA-WSU collaboration. We simulate the inspiral through many orbits, follow the post-merger evolution, and compute the full gravitational wave signal.

  16. SP-100 control drive assembly development

    NASA Technical Reports Server (NTRS)

    Gleason, Thomas; Gilchrist, A. Richard; Schuster, Gary

    1993-01-01

    The SP-100 is an electrical generating nuclear power system for space operation. This paper describes the nuclear reactor control systems and the methods used to assure reliable performance for the 10-year design life. Reliable performance is achieved by redundancy and by selecting highly reliable components and design features. Reliability is quantified by analysis using established reliability data. Areas lacking reliability data are identified for development testing. A specific development test description is provided as an example to demonstrate how this process is meeting the system reliability goals.

  17. SP-100 radiator design trade study

    NASA Technical Reports Server (NTRS)

    Ewell, Richard

    1992-01-01

    A design trade study of the SP-100 heat rejection subsystem (HRSS) was made. A system code was used to evaluate the sensitivity of the HRSS mass and performance to changes. Variations in heat pipe diameter and cross-section, fin length and thickness, armor thickness, and overall configuration and materials were evaluated. The analysis indicates that the minimum system mass occurs for the case with many small diameter heat pipes, with ducting that maximizes the fraction of the heat pipe evaporator perimeter in contact with it.

  18. Wickerhamiella slavikovae sp. nov. and Wickerhamiella goesii sp. nov., two yeast species isolated from natural substrates.

    PubMed

    Hagler, Allen N; Ribeiro, José R A; Pinotti, T; Brandão, Luciana R; Pimenta, Raphael S; Lins, U; Lee, Ching-Fu; Hsieh, Chin-Wen; Lachance, Marc-André; Rosa, Carlos A

    2013-08-01

    Two novel yeast species were isolated during three independent studies of yeasts associated with natural substrates in Brazil and Taiwan. Analysis of the sequences of the D1/D2 domains of the large subunit rRNA gene showed that these novel species belong to the Wickerhamiella clade. The first was isolated from freshwater and a leaf of sugar cane (Saccharum officinarum) in Brazil and from leaves of Wedelia biflora in Taiwan. Described here as Wickerhamiella slavikovae sp. nov., it differs by 56 nucleotide substitutions and 19 gaps in the D1/D2 region of the large subunit rRNA gene from Candida sorbophila, the least divergent species. The second species, named Wickerhamiella goesii sp. nov., was isolated from leaves and the rhizosphere of sugar cane collected in Rio de Janeiro, Brazil. The species differs by 54 nucleotide substitutions and nine gaps in the D1/D2 domains from Candida drosophilae, its least divergent relative. The type strains are Wickerhamiella slavikovae sp. nov. IMUFRJ 52096(T) (= CBS 12417(T) = DBVPG 8032(T)) and Wickerhamiella goesii sp. nov. IMUFRJ 52102(T) (= CBS 12419(T) = DBVPG 8034(T)). PMID:23710055

  19. Leaving the fullerene road: presence and stability of sp chains in sp2

    E-print Network

    Powles, Rebecca

    functional properties. 4 Author to whom any correspondence should be addressed. New Journal of Physics 7 an enormous experimental and theoretical work towards the identification of building blocks of C60 and other], experiments prove that small sp clusters are found in the mass spectra of laser vaporized graphite [9

  20. Mineralization of a Malaysian crude oil by Pseudomonas sp. and Achromabacter sp. isolated from coastal waters

    SciTech Connect

    Ahmad, J.; Ahmad, M.F.

    1995-12-31

    Regarded as being a potentially effective tool to combat oil pollution, bioremediation involves mineralization, i.e., the conversion of complex hydrocarbons into harmless CO{sub 2} and water by action of microorganisms. Therefore, in achieving optimum effectiveness from the application of these products on crude oil in local environments, the capability of the bacteria to mineralize hydrocarbons was evaluated. The microbial laboratory testing of mineralization on local oil degraders involved, first, isolation of bacteria found at a port located on the west coast of Peninsular Malaysia. Subsequently, these bacteria were identified by means of Biomereux`s API 20E and 20 NE systems and later screened by their growth on a Malaysian crude oil. Selected strains of Pseudomonas sp. and Achromabacter sp. were then exposed individually to a similar crude oil in a mineralization unit and monitored for 16 days for release of CO{sub 2}. Pseudomonas paucimobilis was found to produce more CO{sub 2} than Achromobacter sp. When tested under similar conditions, mixed populations of these two taxa produced more CO{sub 2} than that produced by any individual strain. Effective bioremediation of local crude in Malaysian waters can therefore be achieved from biochemically developed Pseudomonas sp. strains.

  1. Das Kopulationsverhalten von Mougeotia polymorpha n. sp. und die Azygotenbildung von Spirogyra dimorpha n. sp

    Microsoft Academic Search

    Lothar Geitler

    1949-01-01

    Zusammenfassung Mougeotia polymorpha n. sp. verhält sich morphologisch und „physiologisch“ völlig isogam. Aus der Analyse von Dreier-, Vierer- und Fünferkopulationen (im ganzen rund 70 Fälle) ergibt sich, daß sie dennoch diözisch ist und somit, nachZygnema circumcarinatum, den zweiten derartigen Fall unter den Konjugaten darstellt.

  2. Clinical problems of sloths (Bradypus sp. and Choloepus sp.) in captivity.

    PubMed

    Diniz, L S; Oliveira, P M

    1999-03-01

    A 20-yr retrospective study of disease prevalence was carried out for 51 sloths (34 Bradypus sp. and 17 Choloepus sp.) at the São Paulo Zoo. A total of 81 clinical disorders were detected, including nutritional (45.7%), digestive (12.3%), and respiratory (12.3%) problems and injuries (6.1%). A definitive diagnosis was not possible in 8.6% of the cases. The incidence of disease varied according to seasonal climate (winter, 32.5%; spring, 24%; summer, 22.9%; autumn, 20.5%), time in captivity (96.4% of diseases occurred within the first 6 mo and 3.6% occurred thereafter), and type of enclosure (quarantine cage, 96.4%; exhibition enclosure, 3.6%). Both young animals (86.7%) and adults (3.2%) were affected. Parasites were identified by fecal examination in 45.4% of animals with clinical illness (Ascaris sp., 80%; Coccidia sp., 20%). Bacteria such as Salmonella enteritidis, Escherichia coli, and Citrobacter freundii were isolated from feces and/or organs. The first 6 mo in captivity are critical for these animals. Proper management and early identification of medical conditions in captivity have implications for sloth population in the wild. PMID:10367647

  3. Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor

    PubMed Central

    2013-01-01

    Background Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. Results 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized. Nucleotide sequences showed 97% and 96% similarity to Methylobacterium sp. AKB-2008-KU9 and Methylobacterium sp. strain 440. The second isolate, likewise, showed 98% similarity to Actinobacterium OR-221. The sequences were deposited in GenBank as Methylobacterium sp. Obi [accession number HQ537130] and Actinobacterium sp. Mayor [accession number JF799090]. Flocculating activity of 95% was obtained in the presence of Ca2+ and heat-stability was exhibited with retention of above 70% activity at 100°C in 30 min. In addition, bioflocculant yield was about 8.203 g/l. A dose of 1 mg/ml of purified bioflocculant was optimal for the clarification of Kaolin suspension (100 ml) following Jar test. FTIR spectrum revealed the presence of carboxyl and hydroxyl functional groups amongst others. Conclusions The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability. PMID:23915393

  4. Environmental interactions and the SP-100 power system

    NASA Technical Reports Server (NTRS)

    Ferguson, Dale C.

    1993-01-01

    Interactions of the SP-100 power system with its expected ambient environments are defined. SP-100 payloads will float 100 V negative of the low Earth orbit (LEO) plasma. Choice of proper geometries and materials will prevent arcing at conductor-insulator junctions in LEO. Care in selecting surface coatings will prevent dielectric breakdown. Sputtering is a concern for long-duration LEO missions. Atomic oxygen durability of SP-100 materials will be tested in ground and flight tests. Evaluation of SP-100 in lunar and planetary environments has begun. The report of a recent workshop on Chemical and Electrical Interactions on Mars identified many of the primary interactions.

  5. The Effect of Acupuncture to SP6 on Skin Temperature Changes of SP6 and SP10: An Observation of “Deqi”

    PubMed Central

    Yang, Jia-Min; Shen, Xiao-Yu; Shen, Song-Xi; Qi, Dan-Dan; Luo, Li; Ren, Xiao-Xuan; Ji, Bo; Zhang, Lu-Fen; Li, Xiao-Hong; Zhu, Jiang

    2014-01-01

    Background. Deqi sensation is a complex but an important component for acupuncture effect. In this study, we tried to observe the relationship between Deqi and skin temperature changes and whether there was some relativity between Deqi and needle stimulations on cold congealing and dysmenorrhea rat model. Thirty-two female Sprague Dawley (SD) rats were randomly divided into four groups (Saline Control Group, Model Group, Group A with strong stimulation, and Group B with small stimulation). Group A and Group B were performed with different stimulations. We found that, compared with saline control group, model group, and Group B, Group A showed that the skin temperature changes on right acupoint SP6 and SP10 increased significantly at 5?min–10?min interval. The skin temperature changes on left SP6 decreased at instant–5?min interval. The skin temperature changes on right SP10 decreased significantly at instant–5?min interval and 10?min–20?min interval. Thermogenic action along Spleen Meridian of Foot Greater Yin was manifested as simultaneous skin temperature increase on right SP6 and SP10 at 5?min–10?min interval after needling SP6, which was helpful to illustrate the relationship between the characteristic of Deqi and needle stimulations. PMID:24665334

  6. J. Phycol. 38, 971982 (2002) FIELD ASSAYS FOR MEASURING NITRATE REDUCTASE ACTIVITY IN ENTEROMORPHA SP.

    E-print Network

    Sherman, Tim

    2002-01-01

    IN ENTEROMORPHA SP. (CHLOROPHYCEAE), ULVA SP. (CHLOROPHYCEAE), AND GELIDIUM SP. (RHODOPHYCEAE)1 Julien Lartigue 2., and Gelidium sp. are described. In opti- mizing each assay, a variety of compounds and assay conditions were., and Gelidium sp. in the field consistently found NR activity. In situ NR activity over 9 consecutive days

  7. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

    PubMed Central

    Sioud, Samiha; Aigle, Bertrand; Karray-Rebai, Ines; Smaoui, Slim; Bejar, Samir; Mellouli, Lotfi

    2009-01-01

    Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch. PMID:19547659

  8. Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae.

    PubMed

    Lin, Yi-Chueh; Uemori, Kazunori; de Briel, Dominique A; Arunpairojana, Vallapa; Yokota, Akira

    2004-09-01

    Seven strains of actinobacteria, isolated from soil, wounds, urine, cow faeces, human blood and butter, were characterized by a polyphasic approach to clarify their taxonomic position. On the basis of chemotaxonomy, 16S rRNA gene analysis and DNA relatedness, strain IAM 14851T can be classified within the cluster of the genus Leucobacter and is proposed as a novel species, Leucobacter albus sp. nov., with strain IAM 14851T (=TISTR 1515T) as the type strain. The other six strains formed a phylogenetically separate branch in the family Microbacteriaceae, having the following characteristics: the major menaquinones are MK-8 to MK-10, the DNA G + C content ranges from 62 to 68 mol%, the diamino acid in the cell wall is diaminobutyric acid and the muramic acid in the peptidoglycan is of the acetyl type. The major fatty acids are 12-methyltetradecanoic acid (anteiso-C(15 : 0)), hexadecanoic acid (C(16 : 0)), 14-methyl-pentadecanoic acid (iso-C(16 : 0)) and 14-methyl-hexadecanoic acid (anteiso-C(17 : 0)). On the basis of morphological, physiological and chemotaxonomic characteristics, together with DNA-DNA hybridization and 16S rRNA gene sequence comparison, the novel genus Zimmermannella gen. nov. is proposed for these six strains. Four novel species are proposed: Zimmermannella helvola sp. nov. (type species; type strain IAM 14726T = NBRC 15775T = DSM 20419T = TISTR 1509T), Zimmermannella alba sp. nov. (type strain IAM 14724T = NBRC 15616T = TISTR 1510T), Zimmermannella bifida sp. nov. (type strain IAM 14848T = TISTR 1511T) and Zimmermannella faecalis sp. nov. (type strain IAM 15030T = NBRC 15706T = ATCC 13722T = TISTR 1514T). PMID:15388726

  9. Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus Rahnella.

    PubMed

    Brady, Carrie; Hunter, Gavin; Kirk, Susan; Arnold, Dawn; Denman, Sandra

    2014-12-01

    Isolations from oak symptomatic of Acute Oak Decline, alder and walnut log tissue, and buprestid beetles in 2009-2012 yielded 32 Gram-negative bacterial strains showing highest gyrB sequence similarity to Rahnella aquatilis and Ewingella americana. Multilocus sequence analysis (using partial gyrB, rpoB, infB and atpD gene sequences) delineated the strains into six MLSA groups. Two MLSA groups contained reference strains of Rahnella genomospecies 2 and 3, three groups clustered within the Rahnella clade with no known type or reference strains and the last group contained the type strain of E. americana. DNA-DNA relatedness assays using both the microplate and fluorometric methods, confirmed that each of the five Rahnella MLSA groups formed separate taxa. Rahnella genomospecies 2 and 3 were previously not formally described due to a lack of distinguishing phenotypic characteristics. In the present study, all five Rahnella MLSA groups were phenotypically differentiated from each other and from R. aquatilis. Therefore we propose to classify the strains from symptomatic oak, alder and walnut and buprestid beetles as: Rahnella victoriana sp. nov. (type strain FRB 225(T)=LMG 27717(T)=DSM 27397(T)), Rahnella variigena sp. nov. (previously Rahnella genomosp. 2, type strain CIP 105588(T)=LMG 27711(T)), Rahnella inusitata sp. nov. (previously Rahnella genomosp. 3, type strain DSM 30078(T)=LMG 2640(T)), Rahnella bruchi sp. nov. (type strain FRB 226(T)=LMG 27718(T)=DSM 27398(T)) and Rahnella woolbedingensis sp. nov. (type strain FRB 227(T)=LMG 27719(T)=DSM 27399(T)). PMID:25264035

  10. Solar Physics & Space Plasma Research Center (SP2RC)

    E-print Network

    Solar Physics & Space Plasma Research Center (SP2RC) University of SheffieldSTFC SSP Intro Summer & Statistics, The University of Sheffield (UK) http://robertus.staff.shef.ac.uk #12;Solar Physics & Space]solitons, applications) ·Conclusions #12;Solar Physics & Space Plasma Research Center (SP2RC) http

  11. Pseudomonas sabulinigri sp. nov., isolated from black beach sand

    E-print Network

    Bae, Jin-Woo

    Pseudomonas sabulinigri sp. nov., isolated from black beach sand Kyoung-Ho Kim,1 Seong Woon Roh,1 the name Pseudomonas sabulinigri sp. nov. is proposed. The type strain is J64T (5KCTC 22137T 5JCM 14963T that strain J64T belongs to the genus Pseudomonas, forming a monophyletic group with Pseudomonas pachastrellae

  12. Nitrogen Control of Atrazine Utilization in Pseudomonas sp. Strain ADP

    Microsoft Academic Search

    V. Garcia-Gonzalez; Fernando Govantes; Liz J. Shaw; Richard G. Burns; Eduardo Santero

    2003-01-01

    Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that support rapid growth of Pseudomonas sp. strain ADP compared to cells cultivated

  13. SP100 power system conceptual design for lunar base applications

    Microsoft Academic Search

    Lee S. Mason; Harvey S. Bloomfield; Donald C. Hainley

    1989-01-01

    A conceptual design is presented for a nuclear power system utilizing an SP-100 reactor and multiple Stirling cycle engines for operation on the lunar surface. Based on the results of this study, it was concluded that this power plant could be a viable option for an evolutionary lunar base. The design concept consists of a 2500 kWt (kilowatt thermal) SP-100

  14. Genome Sequence of a Salinibacterium sp. Isolated from Antarctic Soil

    PubMed Central

    Shin, Seung Chul; Kim, Su Jin; Ahn, Do Hwan; Lee, Jong Kyu; Lee, Hyoungseok; Lee, Jungeun; Hong, Soon Gyu; Lee, Yung Mi

    2012-01-01

    The draft genome of Salinibacterium sp. PAMC 21357, isolated from permafrost soil of Antarctica, was determined. Here we present a 3.1-Mb draft genome sequence of Salinibacterium sp. that could provide further insight into the genetic determination of its cold-adaptive properties. PMID:22493208

  15. Lignin Degradation by Fusarium solani f. sp. glycines

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sudden death syndrome (SDS), caused by the soilborne fungal pathogen Fusarium solani f. sp. glycines, is one of the most important diseases of soybean. Lignin degradation may play a role in the infection, colonization, and survival of the fungus in root tissue . Lignin degradation by F. solani f. sp...

  16. SP100 operational life model. Fiscal Year 1990 annual report

    Microsoft Academic Search

    R. Ewell; H. Awaya

    1990-01-01

    This report covers the initial year`s effort in the development of an Operational Life Model (OLM) for the SP-100 Space Reactor Power System. The initial step undertaken in developing the OLM was to review all available documentation from GE on their plans for the OLM and on the degradation and failure mechanisms envisioned for the SP-100. In addition, the DEGRA

  17. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 2 2010-07-01 2010-07-01 false Sailing Plan (SP). 161.19 Section 161.19 Navigation and Navigable Waters...Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15 minutes before...

  18. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 2 2011-07-01 2011-07-01 false Sailing Plan (SP). 161.19 Section 161.19 Navigation and Navigable Waters...Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15 minutes before...

  19. DIFFERENTIAL ANTAGONISM OF TRICHODERMA SP. AGAINST MACROPHOMINA PHASEOLINA

    Microsoft Academic Search

    Aly A. Aly; Mohamed A. Abdel-Sattar; Moawad R. Omar; Kamel A. Abd-Elsalam

    In view of the ecological hazards of chemicals, pot experiments were conducted to deter- mine the efficacy of Trichoderma sp. against Macrophomina phaseolina. Greenhouse evolution of the interaction between M. phaseolina isolates and Trichoderma sp. isolates revealed a very highly signifi- cant (p = 0.0000). M. phaseolina isolate x antagonist isolate interaction for all the following parameters: preemergence damping-off, postemergence

  20. Longidorus ferrisi n. sp. from California Citrus

    PubMed Central

    Ye, Weimin; Pedram, Majid

    2009-01-01

    In October 1999, the authors received fixed specimens of a species of Longidorus from Howard Ferris found about the roots of a citrus tree in Oakville, Napa County, CA. After determining it to be new a species, we requested additional specimens. The samples contained roughly equal numbers of males and females. Longidorus ferrisi n. sp. is most similar to L. elongatus, but can be distinguished by a greater c-ratio (111-187 vs 73-141), a lesser c´ (0.7-1.1 vs 1.0-1.3), a more offset head, a more posterior guide ring (35-40 vs 30-33 ?m), the presence of sperm in the uterus in mature females, and the approximate 1:1 ratio of females to males. Other similar species include L. artemisiae, L. crassus, L. glycines, and L. milanis. Longidorus ferrisi n. sp. differs from L. artemisiae by a lesser a-ratio (74-102 vs 109-155), a lesser c´ value (0.7-1.1 vs 1.0-1.6), a more posterior guide ring (35-40 vs 27-34 ?m), a longer odontostyle (91-108 vs 84-98 ?m), a wider lip region (16-19 vs 14-17 ?m), wider mid-body (53-69 vs 41-52 ?m), and longer spicules (57-65 vs 39-49 ?m). The new species differs substantially from L. crassus by its lip shape and the presence of males, and differs from L. glycines by a shorter body (4.33-5.97 vs 6.14-8.31 mm), a lesser c´ value (0.7-1.1 vs 0.9-1.4), a narrower lip region (16-19 vs 20-23 ?m), wider mid-body (53-69 vs 39-57 ?m), longer spicules (53-69 vs 45-53 ?m), and fewer supplements (7-11 vs 11-17). Longidorus ferrisi n. sp. differs from L. milanis by a longer body (4.33-5.97vs 3.00-4.90 mm), a greater c value (111-187 vs 86-130), a wider mid-body (53-69 vs 43-56 ?m), a different head shape, and longer spicules (53-69 vs 41-54 ?m). The nuclear 18S ribosomal DNA sequence of this species revealed that this species is unique with respect to all sequenced Longidorus species. PMID:22661782

  1. 78 FR 39828 - Proposed Collection; Comment Request for Forms 8857 and 8857(SP)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ...Comment Request for Forms 8857 and 8857(SP) AGENCY: Internal Revenue Service (IRS...comments concerning Forms 8857 and 8857(SP), Request for Innocent Spouse Relief...1545-1596. Form Numbers: 8857 and 8857(SP). Abstract: Section 6013(e) of...

  2. Unusual Sp1-GC box interaction in a parvovirus promoter.

    PubMed Central

    Pitluk, Z W; Ward, D C

    1991-01-01

    The P4 promoter of the parvovirus minute virus of mice contains a single degenerate GC box sequence which binds the transcription factor Sp1 with high affinity. The two promoters of murine Sp1 were affinity purified, and their interactions with the P4 promoter were examined. Several unusual features were observed. Methylation interference experiments demonstrated that Sp1 makes contacts with both DNA strands, including the central guanine as well as an adenine residue on the cytidine-rich strand of the GC box. UV photocross-linking revealed that the 95- and the 105-kDa promoters cross-link exclusively to opposite strands of the GC box. These results suggest that the phosphorylation of the 95-kDa Sp1 promoter results in a change in the way Sp1 is positioned on the P4 GC box and identifies a high-affinity GC box motif. Images PMID:1942250

  3. SP-100 fuel pin performance analysis

    NASA Astrophysics Data System (ADS)

    Boltax, A.; Sundquist, B. E.

    A modified version of the LIFE code was used to evaluate the performance of a wide range of UO2 and UN fuel pin designs for SP-100 reactors. The analyses covered the available irradiation data on refractory alloy-clad fuel pins and fuel pins for thermoelectric, thermionic, and Stirling engine applications. The LIFE code studies showed that vented UO2 fuel pins with W-26 percent Re cladding can operate with cladding temperatures up to the 1800 K for thermionic reactor applications. At cladding temperatures up to 1500 K, W-26 percent Re and ASTAR-811C cladding can be used for vented or nonvented UO2 fuel pins. Nb-1 percent Zr cladding is not recommended for nonvented pin designs at 1500 K. Similarly, for UN fuel, W-26 percent Re and ASTAR-811C cladding can be used up to 1500 K, whereas, T-111 and Nb-1 percent Zr are limited to temperatures below 1400 K.

  4. Chitinase Production by Streptomyces sp. ANU 6277

    PubMed Central

    Narayana, Kolla J.P.; Vijayalakshmi, Muvva

    2009-01-01

    Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35°C. Culture medium amended with 1% chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield. Chitinase was purified from crude enzyme extract by single step gel filtration by Sephadex G-100. Purified chitinase of the strain exhibited a distinct protein band near 45 kDa by means of SDS-PAGE. PMID:24031419

  5. Analysis of SP-100 critical experiments

    SciTech Connect

    Sapir, J.L.; Brandon, D.I.; Collins, P.J.; Cowan, C.L.; Porter, C.A.; Andre, S.V.

    1988-01-01

    In support of the SP-100 space nuclear power source program, preliminary critical benchmark experiments were performed at the ZPPR facility at ANL-W. These configurations are representative of small, fast-spectrum, BeO-reflected, liquid metal-cooled space reactor designs at a 300-kWe power level. Analyses were performed using MCNP (Monte Carlo) and TWODANT (discrete ordinates) transport codes to calculate system criticality, control worth, and power distribution. Both methods calculated eigenvalues within 0.5% of the experimental results. Internal-poison-rod worth was underpredicted and radial reflector worth was overpredicted by both codes by up to 20%. MCNP-calculated control drum worths were underestimated by approximately 8%. Good agreement with experimental values was observed for /sup 235/U fission and for /sup 238/U fission and capture rates with the best agreement occurring in the fuel region and slightly poorer predictions apparent near BeO moderator. 7 refs., 12 figs.

  6. Winamp 1.92-SP1

    NSDL National Science Digital Library

    Winamp 1.92-SP1, created by Nullsoft, Inc., is a fast, easy-to-use, hi-fidelity music player for Windows 95/98/NT. The player supports numerous audio file formats, most notably MPEG Audio Layer 3 (MP3), which allows near CD-quality sound while compressing a four-minute song into approximately four megabytes. The player has an intuitive interface which includes an equalizer, multi-song programming, and other useful controls. MP3 is becoming widely used as a quality audio compression standard and Winamp is an excellent audio player for use with MP3 and other formats. Winamp is shareware for the Windows 95/98/NT platforms and may be used free of charge for fourteen days, after which time a $10 registration fee is required.

  7. Kazachstania aquatica sp. nov. and Kazachstania solicola sp. nov., novel ascomycetous yeast species.

    PubMed

    Wu, Zuo-Wei; Bai, Feng-Yan

    2005-09-01

    The unidentified strains AS 2.0706(T), preserved in the China General Microbiological Culture Collection Center (CGMCC), Academia Sinica, Beijing, China, and CBS 6904(T), preserved in the Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands, were shown to represent two novel ascomycetous yeast species of the genus Kazachstania by 18S rDNA, internal transcribed spacer (ITS) region (including 5.8S rDNA) and 26S rDNA D1/D2 domain sequence analysis and electrophoretic karyotype comparison. The names Kazachstania aquatica sp. nov. and Kazachstania solicola sp. nov. are proposed for strains AS 2.0706(T) and CBS 6904(T), respectively. Phylogenetically, the two novel species are closely related to Kazachstania aerobia, Kazachstania servazzii and Kazachstania unispora. PMID:16166736

  8. Ogataea ganodermae sp. nov., a methanol-assimilating yeast species isolated from basidiocarps of Ganoderma sp.

    PubMed

    Ji, Zhao-Hui; Bai, Feng-Yan

    2008-06-01

    Three methanol-utilizing yeast strains were isolated from basidiocarps of Ganoderma sp. collected from a tree trunk in Mangshan Mountain, Hunan Province, southern China. These strains formed hat-shaped ascospores in unconjugated and deliquescent asci. Sequence analysis of the large-subunit rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region, electrophoretic karyotype comparison and phenotypic characterization demonstrated that the three strains represent a novel species of the genus Ogataea, which is described as Ogataea ganodermae sp. nov. (type strain SHS 2.1(T) =CGMCC AS 2.3435(T) =CBS 10646(T)). Phylogenetically, the novel species was closely related to Ogataea pini and Ogataea henricii. The latter two taxa with similar D1/D2 sequences were confirmed to represent separate species by ITS sequence and electrophoretic karyotype comparisons. PMID:18523203

  9. Microbial formation of manganese oxides. [Chlamydomonas sp

    SciTech Connect

    Greene, A.C.; Madgwick, J.C. (Univ. of New South Wales, Kensington (Australia))

    1991-04-01

    Microbial manganese oxidation was demonstrated at high Mn{sup 2+} concentrations (5 g/liter) in bacterial cultures in the presence of a microalga. The structure of the oxide produced varied depending on the bacterial strain and mode of culture. A nonaxenic, acid-tolerant microalga, a Chlamydomonas sp., was found to mediate formation of manganite ({gamma}-MnOOH). Bacteria isolated from associations with crude cultures of this alga grown in aerated bioreactors formed disordered {gamma}-MnO{sub 2} from Mn{sup 2+} at concentrations of 5 g/liter over 1 month, yielding 3.3 of a semipure oxide per liter. All algal-bacterial cultures removed Mn{sup 2+} from solution, but only those with the highest removal rates formed an insoluble oxide. While the alga was an essential component of the reaction, a Pseudomonas sp. was found to be primarily responsible for the formation of a manganese precipitate. Medium components - algal biomass and urea - showed optima at 5.7 and 10 g/liters, respectively. The scaled-up culture (50 times) gave a yield of 22.3 g (53 mg/liter/day from a 15-liter culture) of semipure disordered {gamma}MnO{sub 2}, identified by X-ray diffraction and Fourier transform infrared (FTIR) spectroscopy, and had a manganese oxide O/Mn ratio of 1.92. The Mn(IV) content in the oxide was low (30.5%) compared with that of mined or chemically formed {gamma}MnO{sub 2} (ca. 50%). The shortfall in the bacterial oxide manganese content was due to biological and inorganic contaminants.

  10. Decidual expression and localization of human surfactant protein SP-A and SP-D, and complement protein C1q.

    PubMed

    Madhukaran, Shanmuga Priyaa; Kishore, Uday; Jamil, Kaiser; Choolani, Mahesh; Lu, Jinhua

    2015-08-01

    Surfactant proteins SP-A and SP-D, and complement protein C1q are soluble innate immune pattern recognizing molecules. SP-A, SP-D and C1q have an overall similar structure composed of an N-terminal triple-helical collagen region that is followed by a trimeric globular domain. While SP-A and SP-D belong to the collectin family (collagen containing lectin), C1q is the first recognition subcomponent of the classical pathway of the complement system. Recently, SP-A, SP-D and C1q have been considered to play important roles in early and late pregnancy. However, their expression in early human decidua has not been examined. Here, we investigated whether SP-A, SP-D and C1q are expressed within first trimester decidua in humans and their expression is associated with trophoblasts and decidual stromal cells. Decidual samples from women undergoing elective vaginal termination of pregnancy during first trimester were obtained from 25 subjects. Immunohistochemical studies using anti-human SP-A, anti-human SP-D and anti-human C1q antibodies were performed on decidual tissue sections along with anti-vimentin and cytokeratin-7 antibodies to identify stromal cells and trophoblasts. The expression was also examined by immunostaining and PCR using decidual and stromal cells. C1q expression was significantly higher when compared to SP-A and SP-D in the first trimester human decidua. Double immunostaining revealed that all stromal cells and trophoblasts expressed SP-A, SP-D and C1q, while only few invasive trophoblasts expressed C1q. Thus, expression of SP-A, SP-D and C1q in human decidua during first trimester suggests potential role of SP-A, SP-D and C1q during the early stages of pregnancy including implantation, trophoblast invasion and placental development. PMID:25829244

  11. Carbon-Binding Designer Proteins that Discriminate between sp2- and sp3-Hybridized Carbon Surfaces

    PubMed Central

    Coyle, Brandon L.; Rolandi, Marco; Baneyx, François

    2013-01-01

    Robust and simple strategies to directly functionalize graphene- and diamond-based nanostructures with proteins are of considerable interest for biologically driven manufacturing, biosensing and bioimaging. Here, we identify a new set of carbon binding peptides that vary in overall hydrophobicity and charge, and engineer two of these sequences (Car9 and Car15) within the framework of E. coli Thioredoxin 1 (TrxA). We develop purification schemes to recover the resulting TrxA derivatives in a soluble form and conduct a detailed analysis of the mechanisms that underpin the interaction of the fusion proteins with carbonaceous surfaces. Although equilibrium quartz crystal microbalance measurements show that TrxA?Car9 and TrxA?Car15 have similar affinity for sp2-hybridized graphitic carbon (Kd = 50 and 90 nM, respectively), only the latter protein is capable of dispersing carbon nanotubes. Further investigation by surface plasmon resonance and atomic force microscopy reveals that TrxA?Car15 interacts with sp2-bonded carbon through a combination of hydrophobic and ?-? interactions but that TrxA?Car9 exhibits a cooperative mode of binding which relies on a combination of electrostatics and weaker ?-stacking. Consequently, we find that TrxA?Car9 binds equally well to sp2- and sp3-bonded (diamond-like) carbon particles, while TrxA?Car15 is capable of discriminating between the two carbon allotropes. Our results emphasize the importance of understanding both bulk and molecular recognition events when exploiting the adhesive properties of solid-binding peptides and proteins in technological applications. PMID:23510486

  12. Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used for perchlorate reduction by Azospira sp.

    E-print Network

    Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used was shown to be intrinsically different from the enzyme responsible for chlorate and perchlorate [(per)chlo- rate] reduction produced by Azospira sp. KJ based on subunit composition and other enzyme properties

  13. Endosulfan induced alteration in bacterial protein profile and RNA yield of Klebsiella sp. M3, Achromobacter sp. M6, and Rhodococcus sp. M2.

    PubMed

    Singh, Madhu; Singh, Dileep Kumar

    2014-01-30

    Three bacterial strains identified as Klebsiella sp. M3, Achromobacter sp. M6 and Rhodococcus sp. M2 were isolated by soil enrichment with endosulfan followed by shake flask enrichment technique. They were efficiently degrading endosulfan in the NSM (non sulfur medium) broth. Degradation of endosulfan was faster with the cell free extract of bacterial cells grown in the sulfur deficient medium (NSM) supplemented with endosulfan than that of nutrient rich medium (Luria Bertani). In the cell free extract of NSM supplemented with endosulfan as sole sulfur source, a unique band was visualized on SDS-PAGE but not with magnesium sulfate as the sole sulfur source in NSM and LB with endosulfan. Expression of a unique polypeptide band was speculated to be induced by endosulfan under sulfur starved condition. These unique polypeptide bands were identified as OmpK35 protein, sulfate binding protein and outer membrane porin protein, respectively, in Klebsiella sp. M3, Achromobacter sp. M6 and Rhodococcus sp. M2. Endosulfan showed dose dependent negative effect on total RNA yield of bacterial strains in nutrient rich medium. Absence of plasmid DNA indicated the presence of endosulfan metabolizing gene on genomic DNA. PMID:24365874

  14. Survey of Anisakis sp. and Hysterothylacium sp. in sardines and anchovies from the North Adriatic Sea.

    PubMed

    Cavallero, S; Magnabosco, C; Civettini, M; Boffo, L; Mingarelli, G; Buratti, P; Giovanardi, O; Fortuna, C M; Arcangeli, G

    2015-05-01

    The occurrence of larval Anisakidae and Raphidascarididae in anchovies and sardines from the North Adriatic Sea has been estimated. Anisakis pegreffii and Hysterothylacium aduncum were reported, with low prevalence values. In brief, a total amount of 7650 fish specimens collected between September 2011 and 2012 were analysed using three different inspection analyses: a visual inspection of the coelomic cavity, an examination of the viscera exploiting the positive hydro-tropism of the larvae (modified Baermann technique) and enzymatic digestion of muscular tissue pools. Low level of infestation was reported for Anisakis sp. in both in anchovies and sardines, while higher values were reported for Hysterothylacium sp. Subsamples of nematodes collected were characterized at species level using the molecular diagnostic key based on ITS nuclear ribosomal region, and A. pegreffii and H. aduncum were identified. The low prevalence of Anisakis sp. in sardines and anchovies from the North Adriatic Sea could be related to the peculiar distribution of cetaceans and carnivorous zooplankton in the investigated region and could be used as a potential tag to define oily fishes from this specific fishing area as at low-risk for anisakiasis. PMID:25662709

  15. Biophysical activity of an artificial surfactant containing an analogue of surfactant protein (SP)-C and native SP-B.

    PubMed Central

    Palmblad, M; Johansson, J; Robertson, B; Curstedt, T

    1999-01-01

    Natural surfactant preparations containing phospholipids and the hydrophobic surfactant proteins B and C (SP-B and SP-C) are effective in the treatment of respiratory distress syndrome in premature infants. The limited supply, and the risk of infectious agents and immunological reactions have promoted the evaluation of synthetic peptides in surfactant preparations. However, the folding of synthetic SP-C into an alpha-helix is inefficient and alpha-helical SP-C analogues with Val-->Leu substitutions form oligomers. In order to circumvent these problems we have synthesized an SP-C analogue, named SP-C(LKS), which differs from SP-C mainly by the exchange of most of the Val residues in positions 16-28 with Leu residues to promote an alpha-helical conformation, and by the introduction of Lys residues at positions 17, 22 and 27 in order to locate positive charges around the helical circumference and thereby avoid self polymerization. CD spectroscopy showed a spectrum typical for alpha-helical peptides and SDS/PAGE disclosed a single band. The biophysical activity of artificial surfactant preparations containing SP-C(LKS) and phospholipids, with and without native SP-B, was measured using a Wilhelmy balance and a pulsating bubble surfactometer. SP-C(LKS) (3%, w/w) in a mixture of 1, 2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/phosphatidylglycerol/palmitic acid (68:22:9, by wt.) suspended in 150 mM NaCl, showed rapid spreading at the air-liquid interface and produced a surface tension of <1 mN/m at minimum bubble size (gammamin) and 42 mN/m at maximum bubble size (gammamax) in the pulsating bubble surfactometer. The addition of 2% (w/w) SP-B to the preparation reduced the maximum surface tension to 33-35 mN/m, i.e. both gammamin and gammamax values were similar to those of natural surfactant preparations. Optimal in vitro characteristics were also obtained from a preparation containing SP-C(LKS), SP-B, DPPC and phosphatidylglycerol, i.e. when palmitic acid was omitted from the lipid mixture. SP-B containing surfactant preparations made up in Hepes buffer at pH 6.9, instead of in 150 mM NaCl, had similar biophysical activity provided that palmitic acid was omitted, but decreased activity in the presence of palmitic acid. PMID:10191270

  16. Industrial Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP

    E-print Network

    Grissino-Mayer, Henri D.

    Industrial Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA- Math 130 or Math ACT 28 Math 130 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 or Math SAT 630 EF 105 Spring Math 142 or 148 (4) FA, SP, SU English 102 (3) FA, SP, SU Gen Ed (3) FA, SP, SU EF 152 or 158 (4

  17. The sea urchin metallothionein system: Comparative evaluation of the SpMTA and SpMTB metal-binding preferences?

    PubMed Central

    Tomas, Mireia; Domènech, Jordi; Capdevila, Mercè; Bofill, Roger; Atrian, Sílvia

    2013-01-01

    Metallothioneins (MTs) constitute a superfamily of ubiquitous metal-binding proteins of low molecular weight and high Cys content. They are involved in metal homeostasis and detoxification, amongst other proposed biological functions. Two MT isoforms (SpMTA and SpMTB) have been reported in the echinoderm Strongylocentrotus purpuratus (sea urchin), both containing 20 Cys residues and presenting extremely similar sequences, although showing distinct tissular and ontogenic expression patterns. Although exhaustive information is available for the Cd(II)-SpMTA complex, this including the full resolution of its 3D structure, no data has been reported concerning either SpMTA Zn(II) and Cu(I) binding properties, or the characterization of SpMTB at protein level. In this work, both the SpMTA and SpMTB isoforms, as well as their separate ? and ? domains, have been recombinantly synthesized in the presence of Zn(II), Cd(II) or Cu(II), and the corresponding metal complexes have been analyzed using electrospray mass spectrometry, and CD, ICP-AES and UV–vis spectroscopies. The results clearly show a better performance of isoform A when binding Zn(II) and Cd(II), and of isoform B when coordinating Cu(I). Thus, our results confirm the differential metal binding preference of SpMTA and SpMTB, which, together with the reported induction pattern of the respective genes, highlights how also in Echinodermata the MT polymorphism may be linked to the evolution of different physiological roles. PMID:23847757

  18. Luteimonas marina sp. nov., isolated from seawater.

    PubMed

    Baik, Keun Sik; Park, Seong Chan; Kim, Mi Sun; Kim, Eun Mi; Park, Chul; Chun, Jongsik; Seong, Chi Nam

    2008-12-01

    A marine bacterial strain, designated FR1330(T), was isolated from a seawater sample collected near Ganghwa Island, the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FR1330(T) belonged to the Gammaproteobacteria and was related to the genus Luteimonas; its closest neighbours were the type strains of Luteimonas composti (97.9 % sequence similarity) and Luteimonas mephitis (95.0 %). DNA-DNA relatedness values for strain FR1330(T) with Luteimonas composti CC-YY255(T) and Luteimonas mephitis KACC 11391(T) were 33 and 10 %, respectively. Cells of strain FR1330(T) were Gram-negative, aerobic, rod-shaped and oxidase- and catalase-positive. The predominant respiratory lipoquinone was ubiquinone-8. The major fatty acids were branched-chain saturated iso-C(15 : 0) (26.2 %) and unsaturated iso-C(17 : 1)omega9c (26.0 %). The DNA G+C content was 67.6 mol%. On the basis of several phenotypic characteristics, strain FR1330(T) could be differentiated from Luteimonas composti and Luteimonas mephitis. The data obtained from the polyphasic study demonstrated clearly that strain FR1330(T) represents a novel species of the genus Luteimonas. The name Luteimonas marina sp. nov. is proposed, with strain FR1330(T) (=KCTC 12327(T)=JCM 12488(T)=IMSNU 60306(T)) as the type strain. PMID:19060080

  19. Blastocystis sp. from food animals in India.

    PubMed

    Sreekumar, C; Selvaraj, J; Gomathinayagam, S; Thangapandiyan, M; Ravikumar, G; Roy, Parimal; Balachandran, C

    2014-12-01

    Blastocystis, a zoonotic protozoan found in the intestinal tracts of a wide range of animals, has not been reported from non-human hosts from India so far. Organisms indistinguishable from Blastocystis sp. were identified in the Giemsa stained intestinal scrapings collected from carcasses of piglet and poultry that were brought for necropsy to the Central University Laboratory, Chennai. The 'central vacuole forms' of the parasite, with number of nuclei ranging from 1 to 12 were identified. The intensity of infection was low, with less than one organism per oil immersion field, indicating that their presence was unconnected to the cause of death. Caecal scraping was found to be more ideal than duodenal scraping for the diagnosis of Blastocystis, and can be a potential specimen for definitive diagnosis. Identical organisms were also detected in the dung samples of a buffalo calf which showed clinical signs of diarrhoea The presence of Blastocystis in food animals acquires public health significance, as many subtypes of the parasite from poultry and pigs are transmissible to humans. PMID:25320500

  20. Actinomadura geliboluensis sp. nov., isolated from soil.

    PubMed

    Sazak, Anil; Camas, Mustafa; Spröer, Cathrin; Klenk, Hans-Peter; Sahin, Nevzat

    2012-08-01

    A novel actinobacterium, strain A8036(T), isolated from soil, was investigated by using a polyphasic taxonomic approach. The organism formed extensively branched substrate hyphae that generated spiral chains of spores with irregular surfaces. The cell wall contained meso-diaminopimelic acid (type III) and cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinones were MK-9(H(6)) and MK-9(H(4)). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major cellular fatty acids were iso-C(16:0), C(17:1)cis9, C(16:0), C(15:0) and 10-methyl C(17:0). Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain A8036(T) were Actinomadura meyerae DSM 44715(T) (99.23% similarity), Actinomadura bangladeshensis DSM 45347(T) (98.9%) and Actinomadura chokoriensis DSM 45346(T) (98.3%). However, DNA-DNA relatedness and phenotypic data demonstrated that strain A8036(T) could be clearly distinguished from the type strains of all closely related Actinomadura species. Strain A8036(T) is therefore considered to represent a novel species of the genus Actinomadura, for which the name Actinomadura geliboluensis sp. nov. is proposed. The type strain is A8036(T) (?=?DSM 45508(T)?=?KCTC 19868(T)). PMID:22021584

  1. Lactobacillus kitasatonis sp. nov., from chicken intestine.

    PubMed

    Mukai, Takao; Arihara, Keizo; Ikeda, Ami; Nomura, Kazuhito; Suzuki, Fumihiko; Ohori, Hitoshi

    2003-11-01

    Four strains isolated from chicken small intestine and strains JCM 1038 and JCM 1039 (designated as Lactobacillus acidophilus) were characterized by phenotypic and molecular taxonomic methods. They were Gram-positive, catalase-negative, facultatively anaerobic rods that did not produce gas from glucose. These strains had similar phenotypic characteristics and exhibited intergroup DNA relatedness values of >77 %, indicating that they comprised a single species. The 16S rRNA gene sequence of a representative strain, JCM 1039(T) (designated as type strain in this study), was determined and aligned with those of other Lactobacillus species. JCM 1039(T) was placed in the Lactobacillus delbrueckii cluster of the genus Lactobacillus on the basis of phylogenetic analysis and formed an independent cluster that was distinct from its closest neighbours, Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus gallinarum, L. acidophilus and Lactobacillus helveticus. Results of DNA-DNA hybridization experiments and whole-cell protein profiles clearly indicated that these strains represent a novel Lactobacillus species, for which the name Lactobacillus kitasatonis sp. nov. is proposed; the type strain of this species is JCM 1039(T). PMID:14657145

  2. Pontibacter saemangeumensis sp. nov., isolated from seawater.

    PubMed

    Kang, Ji Young; Joung, Yochan; Chun, Jeesun; Kim, Haneul; Joh, Kiseong; Jahng, Kwang Yeop

    2013-02-01

    A gram-negative, rod-shaped, non-motile and pink bacterial strain, designated strain GCM0142(T), was isolated from the confined seawater in the Saemangeum Tide Embankment of South Korea, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis of strain GCM0142(T) indicated that the isolate belonged to the phylum Bacteroidetes and exhibited similarity levels of 94.0-96.4?% to the type strains of recognized Pontibacter species. Strain GCM0142(T) was oxidase- and catalase-positive. The major cellular fatty acids of the novel strain were summed feature 4 (comprising iso-C(17?:?1)I and/or anteiso-C(17?:?1)B, 36.8?%), iso-C(15?:?0) (22.3?%) and summed feature 3 (comprising C(16?:?1)?7c and/or C(16?:?1)?6c, 6.2?%). The DNA G+C content of strain GCM0142(T) was 48.9 mol% and the major quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids (AL1-2), an unknown aminophospholipid, five unknown lipids (L1-5) and an unknown glycolipid. On the basis of the evidence presented, strain GCM0142(T) is considered to represent a novel species of the genus Pontibacter, for which the name Pontibacter saemangeumensis sp. nov. is proposed. The type strain is GCM0142(T) (?=?KACC 16448(T)?=?JCM 17926(T)). PMID:22523168

  3. Respiration patterns of resting wasps (Vespula sp.)

    PubMed Central

    Käfer, Helmut; Kovac, Helmut; Stabentheiner, Anton

    2013-01-01

    We investigated the respiration patterns of wasps (Vespula sp.) in their viable temperature range (2.9–42.4 °C) by measuring CO2 production and locomotor and endothermic activity. Wasps showed cycles of an interburst–burst type at low ambient temperatures (Ta < 5 °C) or typical discontinuous gas exchange patterns with closed, flutter and open phases. At high Ta of >31 °C, CO2 emission became cyclic. With rising Ta they enhanced CO2-emission primarily by an exponential increase in respiration frequency, from 2.6 mHz at 4.7 °C to 74 mHz at 39.7 °C. In the same range of Ta CO2 release per cycle decreased from 38.9 to 26.4 ?l g?1 cycle?1. A comparison of wasps with other insects showed that they are among the insects with a low respiratory frequency at a given resting metabolic rate (RMR), and a relatively flat increase of respiratory frequency with RMR. CO2 emission was always accompanied by abdominal respiration movements in all open phases and in 71.4% of the flutter phases, often accompanied by body movements. Results suggest that resting wasps gain their highly efficient gas exchange to a considerable extent via the length and type of respiration movements. PMID:23399474

  4. Puniceibacterium sediminis sp. nov., from intertidal sediment.

    PubMed

    Zhang, De-Chao; Neuner, Kathrin; Wu, Jiang; Yao, Jianting; Margesin, Rosa

    2015-05-01

    The Gram-stain-negative, rod-shaped bacterial strain designated RU-1-R-18(T) was isolated from intertidal sediment on Sakhalin Island in Russia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RU-1-R-18(T) was related to the genus Puniceibacterium and shared highest sequence similarities with the type strain Puniceibacterium antarcticum KACC 16875(T) (97.9?%). The predominant cellular fatty acid was C18?:?1?7c. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified aminophospholipid and seven unidentified polar lipids. The genomic DNA G+C content of strain RU-1-R-18(T) was 59.1 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain RU-1-R-18(T) represents a novel species of the genus Puniceibacterium , for which the name Puniceibacterium sediminis sp. nov. is proposed (type strain RU-1-R-18(T)?=?LMG 28384(T)?=?DSM 29052(T)). PMID:25687347

  5. Preliminary SP-100/Stirling heat exchanger designs

    SciTech Connect

    Schmitz, P.; Tower, L. [Sverdrup Technology, Inc., Brook Park, OH (United States). Lewis Research Center Group; Dawson, R. [Aerospace Design and Fabrication Inc., Brook Park, OH (United States); Blue, B.; Dunn, P. [National Aeronautics and Space Administration, Cleveland, OH (United States). Lewis Research Center

    1994-09-01

    Analytic modeling of several heat exchanger concepts to couple the SP-100 nuclear reactor lithium loop and the Space Stirling Power Convertor (SSPC) was performed. Four 25 kWe SSPC`s are used to produce the required 100 kW of electrical power. This design work focused on the interface between a single SSPC and the primary lithium loop. Manifolding to separate and collect the four channel flow was not modeled. This work modeled two separate types of heat exchanger interfaces (conductive coupling and radiative coupling) to explore their relative advantages and disadvantages. The minimum mass design of the conductively coupled concepts was 18 kg or 0.73 kg/kWe for a single 25 kWe convertor. The minimum mass radiatively coupled concept was 41 kg or 1.64 kg/kWe. The direct conduction heat exchanger provides a lighter weight system because of its ability to operate the Stirling convertor evaporator at higher heat fluxes than those attainable by the radiatively coupled systems. Additionally the conductively coupled concepts had relatively small volumes and provide potentially simpler assembly. Their disadvantages were the tight tolerances and material joining problems associated with this refractory to superalloy interface. The advantages of the radiatively coupled designs were the minimal material interface problems.

  6. Nonomuraea muscovyensis sp. nov., isolated from soil.

    PubMed

    Ozdemir-Kocak, Fadime; Isik, Kamil; Veyisoglu, Aysel; Tatar, Demet; Sahin, Nevzat

    2014-07-01

    A novel actinomycete, strain FMN03(T), was isolated from a soil sample collected from Yuga Zapadnaya South-West Forest Park, Moscow, Russia. The isolate had chemical and morphological properties typical of members of the genus Nonomuraea and formed a distinct 16S rRNA gene subclade with the type strains Nonomuraea roseoviolacea subsp. carminata NBRC 15903(T) and Nonomuraea roseoviolacea subsp. roseoviolacea NBRC 14098(T). The organism formed extensively branched substrate and aerial hyphae, which generated spiral chains of spores with smooth surfaces. The cell wall contained meso-diaminopimelic acid and the whole cell sugars were glucose, galactose and trace amounts of madurose, mannose and xylose. The polar lipids were phosphatidylethanolamine, hydroxyphosphatidylethanolamine, four unidentified phospholipids, four unidentified glycolipids and one unidentified lipid. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C16 : 0 2-OH, C17 : 0 10-methyl, C17 : 1 cis9 and iso-C16 : 0. Analyses of its morphological, physiological and biochemical characteristics, together with DNA-DNA relatedness data, confirmed that strain FMN03(T) is a representative of a novel species of the genus Nonomuraea, which is distinct from closely related reference strains. Strain FMN03(T) (?= DSM 45913(T)?= KCTC 29233(T)) is proposed as the type strain of a novel species, for which the name Nonomuraea muscovyensis sp. nov. is proposed. PMID:24801152

  7. Sphingomonas humi sp. nov., isolated from soil.

    PubMed

    Yi, Tae Hoo; Han, Chang-Kyun; Srinivasan, Sathiyaraj; Lee, Kang Jin; Kim, Myung Kyum

    2010-04-01

    A gram-negative, non-motile, non-spore-forming, small, orange, rod-shaped bacterium was isolated from soil in South Korea and characterized to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequence examination revealed that strain PB323(T) belongs to the family Sphingomonadaceae. The highest degree of sequence similarity was found with Sphingomonas kaistensis PB56(T) (98.9%), followed by Sphingomonas astaxanthinifaciens TDMA-17(T) (98.3%). Chemotaxonomic characteristics (the G+C content of the genomic DNA 69.0 mol%, Q-10 quinone system, C(18:1) omega7c/omega9t/omega12t, C(16:1) omega7c/C(15:0) iso 2OH, C(17:1) omega6c, and C16:0 as the major fatty acids) corroborated assignment of strain PB323(T) to the genus Sphingomonas. Results of physiological and biochemical tests clearly demonstrate that strain PB323(T) represents a distinct species and support its affiliation with the genus Sphingomonas. Based on these data, PB323(T) (=KCTC 12341(T) =JCM 16603T =KEMB 9004-003(T)) should be classified as a type strain of a novel species, for which the name Sphingomonas humi sp. nov. is proposed. PMID:20437147

  8. Campylobacter iguaniorum sp. nov., isolated from reptiles.

    PubMed

    Gilbert, Maarten J; Kik, Marja; Miller, William G; Duim, Birgitta; Wagenaar, Jaap A

    2015-03-01

    During sampling of reptiles for members of the class Epsilonproteobacteria, strains representing a member of the genus Campylobacter not belonging to any of the established taxa were isolated from lizards and chelonians. Initial amplified fragment length polymorphism, PCR and 16S rRNA sequence analysis showed that these strains were most closely related to Campylobacter fetus and Campylobacter hyointestinalis. A polyphasic study was undertaken to determine the taxonomic position of five strains. The strains were characterized by 16S rRNA and atpA sequence analysis, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and conventional phenotypic testing. Whole-genome sequences were determined for strains 1485E(T) and 2463D, and the average nucleotide and amino acid identities were determined for these strains. The strains formed a robust phylogenetic clade, divergent from all other species of the genus Campylobacter. In contrast to most currently known members of the genus Campylobacter, the strains showed growth at ambient temperatures, which might be an adaptation to their reptilian hosts. The results of this study clearly show that these strains isolated from reptiles represent a novel species within the genus Campylobacter, for which the name Campylobacter iguaniorum sp. nov. is proposed. The type strain is 1485E(T) (?=?LMG 28143(T)?=?CCUG 66346(T)). PMID:25574036

  9. Flavisolibacter swuensis sp. nov. Isolated from Soil.

    PubMed

    Joo, Eun Sun; Cha, Seho; Kim, Myung Kyum; Jheong, Weonhwa; Seo, Taegun; Srinivasan, Sathiyaraj

    2015-07-01

    A Gram-staining-negative, non-motile, non-spore-forming, and rod-shaped bacterium designated as strain SR2-4-2(T) was isolated from soil in South Korea. Phylogenetic analysis based on 16S rRNA gene sequence of strain SR2-4-2(T) revealed that it belonged to the genus of Flavisolibacter, family of Chitinophagaceae, and class of Sphingobacteriia. It shared sequence similarities with Flavisolibacter ginsengisoli Gsoil 643(T) (96.4%), Flavisolibacter ginsengiterrae Gsoil 492(T) (96.3%), and Flavisolibacter rigui 02SUJ3(T) (93.0%). Chemotaxonomic data revealed that its predominant fatty acids were iso-C15:0 (26.4%) and iso-C17:0 3OH (10.7%). Its major polar lipid was phosphatidylethanolamine (PE) and its predominant respiratory quinone was MK-7. The G+C content of genomic DNA of the strain SR2-4-2(T) DNA was 45.0%. Based on the phylogenetic, chemotaxonomic, and phenotypic data, the strain SR2-4-2(T) (=JCM 19974(T) =KEMB 9004-156(T)) is classified as a type strain of a novel species for which the name of Flavisolibacter swuensis sp. nov. is proposed. PMID:26115992

  10. Combinations of fluorescently labeled pulmonary surfactant proteins SP-B and SP-C in phospholipid films.

    PubMed Central

    Nag, K; Taneva, S G; Perez-Gil, J; Cruz, A; Keough, K M

    1997-01-01

    Hydrophobic pulmonary surfactant (PS) proteins B (SP-B) and C (SP-C) modulate the surface properties of PS lipids. Epifluorescence microscopy was performed on solvent-spread monolayers of fluorescently labeled porcine SP-B (R-SP-B, labeled with Texas Red) and SP-C (F-SP-C, labeled with fluorescein) in dipalmitoylphosphatidylcholine (DPPC) (at protein concentrations of 10 and 20 wt%, and 10 wt% of both) under conditions of cyclic compression and expansion. Matrix-assisted laser desorption/ionization (MALDI) spectroscopy of R-SP-B and F-SP-C indicated that the proteins were intact and labeled with the appropriate fluorescent probe. The monolayers were compressed and expanded for four cycles at an initial rate of 0.64 A2 x mol(-1) x s(-1) (333 mm2 x s x [-1]) up to a surface pressure pi approximately 65 mN/m, and pi-area per residue (pi-A) isotherms at 22 +/- 1 degrees C were obtained. The monolayers were microscopically observed for the fluorescence emission of the individual proteins present in the film lipid matrix, and their visual features were video recorded for image analysis. The pi-A isotherms of the DPPC/protein monolayers showed characteristic "squeeze out" effects at pi approximately 43 mN/m for R-SP-B and 55 mN/m for F-SP-C, as had previously been observed for monolayers of the native proteins in DPPC. Both proteins associated with the expanded (fluid) phase of DPPC monolayers remained in or associated with the monolayers at high pi (approximately 65 mN/m) and redispersed in the monolayer upon its reexpansion. At comparable pi and area/molecule of the lipid, the proteins reduced the amounts of condensed (gel-like) phase of DPPC monolayers, with F-SP-C having a greater effect on a weight basis than did R-SP-B. In any one of the lipid/protein monolayers the amounts of the DPPC in condensed phase were the same at equivalent pi during compression and expansion and from cycle to cycle. This indicated that only minor loss of components from these systems occurred between compression-expansion cycles. This study indicates that hydrophobic PS proteins associate with the fluid phase of DPPC in films, some proteins remain at high surface pressures in the films, and such lipid-protein films can still attain high pi during compression. Images FIGURE 2 FIGURE 6 PMID:9168039

  11. Telosticta iban sp. nov. from Sarawak (Odonata: Zygoptera: Platystictidae) .

    PubMed

    Dow, Rory A

    2014-01-01

    T. iban sp. nov. is described from the Lanjak Entimau Wildlife Sanctuary in Sarawak, Malaysian Borneo. Both sexes can be distinguished from all other species of Telosticta by the form of the antehumeral markings. PMID:24872033

  12. Users guide for the ANL IBM SP1

    SciTech Connect

    Gropp, W.; Lusk, E.; Pieper, S.C.

    1994-10-01

    This guide presents the features of the IBM SP1 installed in the Mathematics and Computer Science Division at Argonne National Laboratory. The guide describes the available hardware and software, access policies, and hints for using the system productively.

  13. Launch vehicle integration requirements for SP-100. Technical information report

    SciTech Connect

    Shaw, L.T. Jr.; Womack, J.R.

    1984-03-01

    SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS) Space Shuttle System into a near-earth orbit, deployed, and through upper stage propulsion burn(s) be inserted/transferred to its mission orbit. The nature of the advanced design SP-100 payloads using this power plant are physically and functionally compatible with the NSTS and meet the safety requirements thereof. The purpose of this document is to define and present the requirements and interface provisions that, when satisfied, will ensure technical compatibility between SP-100 systems and the NSTS.

  14. Stimulation of bioluminescence in Noctiluca sp. using controlled temperature changes.

    PubMed

    Han, Jing; Li, GuiJuan; Liu, HuanYing; Hu, HaoHao; Zhang, XueGang

    2013-01-01

    Bioluminescence induced by multifarious stimuli has long been observed and is remains under investigation because of its great complexity. In particular, the exact mechanism underlying bioluminescence is not yet fully understood. This work presents a new experimental method for studying Noctiluca sp. bioluminescence under temperature change stimulation. It is a study of Noctiluca sp. bioluminescence using controlled temperature changes in a tank. A characteristic of this experiment is the large volume of water used (1?m(3) in a tank of 2?×?1?×?1?m). Temperature changes were controlled by two methods. In the first, a flask filled with hot water was introduced into the tank and in the second, a water heater was used in the tank. Temperature changes were recorded using sensors. Noctiluca sp. bioluminescence was recorded using a Canon 5D Mark II and this allowed the characteristics of Noctiluca sp. bioluminescence under temperature change stimulation to be monitored. PMID:23001957

  15. Genome Sequence of the Soil Bacterium Jantinobacterium sp. KBS0711

    PubMed Central

    Shoemaker, William R.; Muscarella, Mario E.

    2015-01-01

    We present a draft genome of Janthinobacterium sp. KBS0711 that was isolated from agricultural soil. The genome provides insight into the ecological strategies of this bacterium in free-living and host-associated environments.

  16. A Systematic and Developmental Analysis of Arthropitys deltoides Sp. Nov.

    E-print Network

    Cichan, Michael A.; Taylor, Thomas N.

    1983-06-01

    Arthropitys deltoides sp. nov. is described from Pennsvlvanian sediments of southeastern Kentucky. The axes are characterized by parenchymatous interfascicular rays that broaden conspicuously toward the periphery of the wood and secondary tracheids...

  17. Liquid metal testing at ETEC for the SP-100 program

    NASA Astrophysics Data System (ADS)

    Triumph, Therese F.

    The technology goals of the SP-100 space program include the design, fabrication, testing and development of a space based power system. The purpose of the Liquid Metal Component Test Program at ETEC is to test the high temperature, lithium cooled, refractory metal SP-100 components at operating conditions. This requires ground testing in a high vacuum environment in order to avoid oxygen embrittlement of the Nb - 1 percent Zr refractory metal. What articles are to be tested and how, is presented.

  18. Sp1- and Krüppel-like transcription factors

    Microsoft Academic Search

    Joanna Kaczynski; Tiffany Cook; Raul Urrutia

    2003-01-01

    SUMMARY: Sp1-like proteins and Krüppel-like factors (KLFs) are highly related zinc-finger proteins that are important components of the eukaryotic cellular transcriptional machinery. By regulating the expression of a large number of genes that have GC-rich promoters, Sp1-like\\/KLF transcription regulators may take part in virtually all facets of cellular function, including cell proliferation, apoptosis, differentiation, and neoplastic transformation. Individual members of

  19. Lethrus (Lethrus) schneideri sp. n. (Coleoptera, Geotrupidae) from Greece

    PubMed Central

    Král, David; Hillert, Oliver; Drožová, Dana; Šípek, Petr

    2013-01-01

    Abstract Lethrus (Lethrus) schneideri Král & Hillert, sp. n. from Thrace, Greece, is described. The new species is morphologically most similar and probably closely related to Lethrus (Lethrus) apterus (Laxmann, 1770) and Lethrus (Lethrus) ares Král, Rejsek & Schneider, 2001. Diagnostic characters (shape of mandibles, ventral mandible processes, pronotum and parameres) are illustrated. Character matrix for separation of males of the Lethrus species closely related to Lethrus schneideri Král & Hillert, sp. n. and geographic ranges for all species studied are mapped. PMID:24146588

  20. SP100 Ground Engineering Systems: Test facility control systems

    Microsoft Academic Search

    W. F. Carlson; J. B. Henshall; K. L. Hampsten

    1988-01-01

    One of the major objectives of the current phase of the SP-100 program is to demonstrate the performance of a full-scale, prototypic nuclear subsystem of a 100 kWe space nuclear power supply. Such a test is planned at the SP-100 Test Site located at U.S. Department of Energy facilities near Richland, Washington. Considerations involved in selecting a design for the

  1. Progress on the SP100 Power Conversion Subsystem

    Microsoft Academic Search

    Christopher England; Richard C. Ewell

    1994-01-01

    The SP-100 Space Reactor Power System converts reactor heat to electricity by thermoelectric conversion, a static method proven in many long-lived spacecraft such as Voyager. For SP-100, the specific power of the thermoelectric converter must be greatly increased over existing technology. Non-useful temperature drops need to be minimized to reduce the mass of the heat rejection subsystem. Both these objectives

  2. Mechanism Based Anticancer Drugs that Degrade Sp Transcription Factors 

    E-print Network

    Chadalapaka, Gayathri

    2013-03-14

    the formation of low-grade, noninvasive papillary urothelial tumors (65). ................20 Figure 4. Genetic progression of pancreatic adenocarcinoma (114). ................33 Figure 5. Mechanism of action of Vinca alkaloids and taxoids...?B...........................105 Figure 17. Role of Sp protein and NF?B on protein expression and the effects of curcumin on Sp-DNA binding and bladder tumor growth. 109 Figure 18. Effects of gefitinib (A), BA (B) and curcumin (C) on cell survival. ...122 Figure 19. BA...

  3. Eustrongylides sp. epizootic in young common egrets (Casmerodius albus)

    USGS Publications Warehouse

    Roffe, Thomas J.

    1988-01-01

    In May 1985, epizootic mortality was reported in common egrets (Casmerodius albus) on Avery Island, Louisiana. Subsequent investigation revealed that more than 400 birds died. Severe peritoneal nematodiasis (Eustrongylides sp.) was found on postmortem examination. A nearby breeding rookery on the same island was apparently unaffected. Reasons for this selective mortality are presented. Three other reports of epizootic mortality due to Eustrongylides sp. have been reported. This is the first report of this type of epizootic in gulf-coast birds.

  4. Launch vehicle integration requirements for SP100. Technical information report

    Microsoft Academic Search

    L. T. Jr. Shaw; J. R. Womack

    1984-01-01

    SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS)

  5. A new species of Loxosomatidae (Entoprocta, Solitaria) from the White Sea: Loxosomella unicornis sp. nov.

    PubMed

    Borisanova, Anastasiya O; Krylova, Elena M

    2014-01-01

    A new solitary entoproct, Loxosomella unicornis sp. nov., is described. The species was found on the gymnolaemate bryozoans Cribrilina sp. and Electra sp. in Kandalaksha Bay, White Sea. Loxosomella unicornis sp. nov. is a medium-sized species with a total length up to 650 µm, eight tentacles and a conspicuous horn-shaped appendage on the top part of calyx.  PMID:25283409

  6. Mike Rose SP10 UCSD FPGA FPGA PCIePCIe BandwidthBandwidth

    E-print Network

    Mike Rose SP10 UCSD FPGA FPGA PCIePCIe BandwidthBandwidth Mike Rose 6/9/10 SP10 UCSD 1 #12;Mike Rose SP10 UCSD Why use an FPGA?Why use an FPGA? · Field Programmable Gate Array (FPGA) · Programmable detection ­ 60 fps Point Tracker 4 #12;Mike Rose SP10 UCSD FPGA CommunicationFPGA Communication · Why

  7. Morphology of three new colonial sessile peritrich ciliates, Pseudepistylis paramphora n. sp., Zoothamnium paranii n. sp. and Z. hartwigi n. sp., with notes on Epicarchesium variabile (Ciliophora, Peritrichia).

    PubMed

    Sun, Ping; Warren, Alan; Al-Farraj, Saleh A; Song, Weibo

    2015-04-01

    Four colonial sessile peritrichs were discovered during a survey of ciliate diversity of coastal waters of the Yellow Sea, Shandong Province, China. Following studies of their living morphology, infraciliature and silverline system, three were identified as new members of the genera Pseudepistylis and Zoothamnium. Pseudepistylis paramphora n. sp. is the second species assigned to the genus, which is characterized by its rigid, dichotomously branched stalk, reticulate silverline system and marine habitat. Zoothamnium paranii n. sp. was isolated from brackish water and Z. hartwigi n. sp. was collected from marine water. Zoothamnium paranii is characterized largely by its highly variable zooid size and having an extremely long infundibular polykinety 3. Zoothamnium hartwigi is separated from its congeners by having two types both of peristomial lip and infundibular polykinety 3. We also supply supplementary morphometric data and photomicrographs of Epicarchesium variabile based on examinations of specimens in vivo and after silver staining. PMID:25855144

  8. Evidence for cooperative mineralization of diuron by Arthrobacter sp. BS2 and Achromobacter sp. SP1 isolated from a mixed culture enriched from diuron exposed environments.

    PubMed

    Devers-Lamrani, Marion; Pesce, Stéphane; Rouard, Nadine; Martin-Laurent, Fabrice

    2014-12-01

    Diuron was found to be mineralized in buffer strip soil (BS) and in the sediments (SED) of the Morcille river in the Beaujolais vineyard repeatedly treated with this herbicide. Enrichment cultures from BS and SED samples led to the isolation of three bacterial strains transforming diuron to 3,4-dichloroaniline (3,4-DCA) its aniline derivative. 16S rRNA sequencing revealed that they belonged to the genus Arthrobacter (99% of similarity to Arthrobacter globiformis strain K01-01) and were designated as Arthrobacter sp. BS1, BS2 and SED1. Diuron-degrading potential characterized by sequencing of the puhA gene, characterizing the diuron-degradaing potential, revealed 99% similarity to A. globiformis strain D47 puhA gene isolated a decade ago in the UK. These isolates were also able to use chlorotoluron for their growth. Although able to degrade linuron and monolinuron to related aniline derivatives they were not growing on them. Enrichment cultures led to the isolation of a strain from the sediments entirely degrading 3,4-DCA. 16S rRNA sequence analysis showed that it was affiliated to the genus Achromobacter (99% of similarity to Achromobacter sp. CH1) and was designated as Achromobacter sp. SP1. The dcaQ gene encoding enzyme responsible for the transformation of 3,4-DCA to chlorocatechol was found in SP1 with 99% similarity to that of Comamonas testosteroni WDL7. This isolate also used for its growth a range of anilines (3-chloro-4-methyl-aniline, 4-isopropylaniline, 4-chloroaniline, 3-chloroaniline, 4-bromoaniline). The mixed culture composed of BS2 and SP1 strains entirely mineralizes (14)C-diuron to (14)CO2. Diuron-mineralization observed in the enrichment culture could result from the metabolic cooperation between these two populations. PMID:25061887

  9. Co-infection of the Siberian hamster (Phodopus sungorus) with a novel Helicobacter sp. and Campylobacter sp.

    PubMed

    Nagamine, Claude M; Shen, Zeli; Luong, Richard H; McKeon, Gabriel P; Ruby, Norman F; Fox, James G

    2015-05-01

    We report the isolation of a novel helicobacter isolated from the caecum of the Siberian hamster (Phodopus sungorus). Sequence analysis showed 97?% sequence similarity to Helicobacter ganmani. In addition, we report the co-infection of these Siberian hamsters with a Campylobacter sp. and a second Helicobacter sp. with 99?% sequence similarity to Helicobacter sp. flexispira taxon 8 (Helicobacter bilis), a species isolated previously from patients with bacteraemia. Gross necropsy and histopathology did not reveal any overt pathological lesions of the liver and gastrointestinal tract that could be attributed to the Helicobacter or Campylobacter spp. infections. This is the first helicobacter to be identified in the Siberian hamster and the first report of co-infection of Helicobacter spp. and Campylobacter sp. in asymptomatic Siberian hamsters. PMID:25752854

  10. Paenibacillus pasadenensis sp. nov. and Paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility.

    PubMed

    Osman, Shariff; Satomi, Masataka; Venkateswaran, Kasthuri

    2006-07-01

    Two novel spore-forming, Gram-positive, mesophilic, heterotrophic bacteria representing two novel species were isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) at Pasadena, CA, USA. The incidence of similar strains was examined by screening the growing collection of isolates ( approximately 400 strains) obtained from the JPL-SAF using species-specific PCR primer sets designed from the 16S rRNA gene sequences of strains SAFN-016(T) and SAFN-007(T). Phylogenetic analysis of 16S rRNA gene sequences placed these novel isolates within the genus Paenibacillus. Two strains, SAFN-016(T) and SAFN-125, shared 98 % 16S rRNA gene sequence similarity with Paenibacillus timonensis and 97 % similarity with Paenibacillus macerans. Strain SAFN-007(T) showed 95.2 % 16S rRNA gene sequence similarity with Paenibacillus kobensis, its nearest phylogenetic neighbour. The results of DNA-DNA hybridization, physiological tests and biochemical analysis allowed genotypic and phenotypic differentiation of the isolates from currently recognized Paenibacillus species. Strain SAFN-007(T) and strains SAFN-016(T) and SAFN-125 are representatives of two separate novel species, for which the names Paenibacillus pasadenensis sp. nov. (type strain SAFN-007(T)=ATCC BAA-1211(T)=NBRC 101214(T)) and Paenibacillus barengoltzii sp. nov. (type strain SAFN-016(T)=ATCC BAA-1209(T)=NBRC 101215(T)) are proposed. PMID:16825621

  11. Aminobacter ciceronei sp. nov. and Aminobacter lissarensis sp. nov., isolated from various terrestrial environments

    USGS Publications Warehouse

    McDonald, I.R.; Kampfer, P.; Topp, E.; Warner, K.L.; Cox, M.J.; Connell, Hancock T.L.; Miller, L.G.; Larkin, M.J.; Ducrocq, V.; Coulter, C.; Harper, D.B.; Murrell, J.C.; Oremland, R.S.

    2005-01-01

    The bacterial strains IMB-1T and CC495T, which are capable of growth on methyl chloride (CH3Cl, chloromethane) and methyl bromide (CH3Br, bromomethane), were isolated from agricultural soil in California fumigated with CH3Br, and woodland soil in Northern Ireland, respectively. Two pesticide- /herbicide-degrading bacteria, strains ER2 and C147, were isolated from agricultural soil in Canada. Strain ER2 degrades N-methyl carbamate insecticides, and strain C147 degrades triazine herbicides widely used in agriculture. On the basis of their morphological, physiological and genotypic characteristics, these four strains are considered to represent two novel species of the genus Aminobacter, for which the names Aminobacter ciceronei sp. nov. (type strain IMB-1T=ATCC 202197T=CIP 108660T=CCUG 50580T; strains ER2 and C147) and Aminobacter lissarensis sp. nov. (type strain CC495T=NCIMB 13798T=CIP 108661T=CCUG 50579T) are proposed. ?? 2005 IUMS.

  12. Collimonas arenae sp. nov. and Collimonas pratensis sp. nov., isolated from (semi-)natural grassland soils.

    PubMed

    Höppener-Ogawa, S; de Boer, W; Leveau, J H J; van Veen, J A; de Brandt, E; Vanlaere, E; Sutton, H; Dare, D J; Vandamme, P

    2008-02-01

    A polyphasic taxonomic study was performed to compare 26 novel bacterial isolates obtained from (semi-)natural grassland soils and a heathland soil in the Netherlands with 16 strains that had previously been assigned to the genus Collimonas. Genomic fingerprinting (BOX-PCR), whole-cell protein electrophoresis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry of intact cells and physiological characterization (Biolog) of the isolates confirmed the existence of different strain clusters (A-D) within the genus Collimonas. Until now, only cluster C strains have been formally classified, as Collimonas fungivorans. In this study, DNA-DNA hybridizations were performed with a selection of strains representing the four clusters. The results showed that cluster B strains also belong to C. fungivorans and that strains of clusters A and D represent two novel species within the genus Collimonas. The latter novel species could be differentiated by means of phenotypic and genotypic characteristics and are classified as Collimonas arenae sp. nov. (cluster A; type strain Ter10(T) =LMG 23964(T) =CCUG 54727(T)) and Collimonas pratensis sp. nov. (cluster D; type strain Ter91(T) =LMG 23965(T) =CCUG 54728(T)). PMID:18218941

  13. Streptomyces graminisoli sp. nov. and Streptomyces rhizophilus sp. nov., isolated from bamboo (Sasa borealis) rhizosphere soil.

    PubMed

    Lee, Hyo-Jin; Whang, Kyung-Sook

    2014-05-01

    Four strains of actinomycete, designated strains JR-19T, JR-12, JR-29 and JR-41T were isolated from bamboo (Sasa borealis) rhizosphere soil. Phylogenetic, morphological, chemotaxonomic and phenotypic analysis demonstrated that the four strains belong to the genus Streptomyces. Microscopic observation revealed that the four strains produced spirales spore chains with spiny surfaces. The cell-wall peptidoglycan of the four strains contained ll-diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates mainly contained glucose and ribose. The predominant menaquinones were MK-9 (H6) and MK-9 (H8). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that these strains and the members of the genus Streptomyces exhibited moderately high 16S rRNA gene sequence similarities of 98.3-99.3%, with the most closely related strains being Streptomyces shenzhenensis 172115T and Streptomyces gramineus JR-43T. Based on the phenotypic and genotypic data, the four strains are considered to represent two novel species of the genus Streptomyces, for which the names Streptomyces graminisoli sp. nov. [to accommodate strains JR-19T (type strain; =KACC 16472T=NBRC 108883T), JR-12 (=KACC 16471) and JR-29 (=KACC 16473)] and Streptomyces rhizophilus sp. nov. [for strain JR-41T (=KACC 16580T=NBRC 108885T)] are proposed. PMID:24478213

  14. Listeria booriae sp. nov. and Listeria newyorkensis sp. nov., from food processing environments in the USA.

    PubMed

    Weller, Daniel; Andrus, Alexis; Wiedmann, Martin; den Bakker, Henk C

    2015-01-01

    Sampling of seafood and dairy processing facilities in the north-eastern USA produced 18 isolates of Listeria spp. that could not be identified at the species-level using traditional phenotypic and genotypic identification methods. Results of phenotypic and genotypic analyses suggested that the isolates represent two novel species with an average nucleotide blast identity of less than 92% with previously described species of the genus Listeria. Phylogenetic analyses based on whole genome sequences, 16S rRNA gene and sigB gene sequences confirmed that the isolates represented by type strain FSL M6-0635(T) and FSL A5-0209 cluster phylogenetically with Listeria cornellensis. Phylogenetic analyses also showed that the isolates represented by type strain FSL A5-0281(T) cluster phylogenetically with Listeria riparia. The name Listeria booriae sp. nov. is proposed for the species represented by type strain FSL A5-0281(T) (?=DSM 28860(T)?=LMG 28311(T)), and the name Listeria newyorkensis sp. nov. is proposed for the species represented by type strain FSL M6-0635(T) (?=DSM 28861(T)?=LMG 28310(T)). Phenotypic and genotypic analyses suggest that neither species is pathogenic. PMID:25342111

  15. Novosphingobium marinum sp. nov., isolated from seawater.

    PubMed

    Huo, Ying-Yi; You, Hong; Li, Zheng-Yang; Wang, Chun-Sheng; Xu, Xue-Wei

    2015-02-01

    A Gram-stain-negative, aerobic, short rod-shaped bacterium, strain LA53(T), was isolated from a deep-sea water sample collected from the eastern Pacific Ocean. Strain LA53(T) grew in the presence of 0-7.0 % (w/v) NaCl and at 15-37 °C; optimum growth was observed with 1.0-2.0 % (w/v) NaCl and at 35 °C. Chemotaxonomic analysis showed ubiquinone-10 as the predominant respiratory quinone, C18 : 1?7c and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1?7c) as major fatty acids, and diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid as major polar lipids. The genomic DNA G+C content was 57.7 mol%. Phylogenetic analyses revealed that strain LA53(T) belongs to the genus Novosphingobium. 16S rRNA gene sequence similarities between strain LA53(T) and the type strains of species of the genus Novosphingobium with validly published names ranged from 93.1 to 96.3 %. In addition, strain LA53(T) could be differentiated from Novosphingobium pentaromativorans DSM 17173(T) and Novosphingobium indicum DSM 23608(T) as well as the type strain of the type species of the genus, Novosphingobium capsulatum DSM 30196(T), by some phenotypic characteristics, including hydrolysis of substrates, utilization of carbon sources and susceptibility to antibiotics. On the basis of phenotypic and genotypic data, strain LA53(T) represents a novel species within the genus Novosphingobium, for which the name Novosphingobium marinum sp. nov. is proposed. The type strain is LA53(T) (?= CGMCC 1.12918(T)?= JCM 30307(T)). PMID:25424486

  16. Shewanella dokdonensis sp. nov., isolated from seawater.

    PubMed

    Sung, Hye-Ri; Yoon, Jung-Hoon; Ghim, Sa-Youl

    2012-07-01

    A novel bacterial strain, designated UDC329(T), was isolated from a sample of seawater collected at Dong-do, on the coast of Dokdo Island, in the East Sea of the Republic of Korea. The Gram-staining-negative, motile, facultatively anaerobic, non-spore-forming rods of the strain developed into dark orange-yellow colonies. The strain grew optimally between 25 and 30 °C, with 1% (w/v) NaCl and at pH 7. It grew in the absence of NaCl, but not with NaCl at >7% (w/v). The predominant menaquinone was MK-7, the predominant ubiquinones were Q-7 and Q-8, and the major fatty acids were iso-C(15:0) (33.52%) and C(17:1)?8c (11.73%). The genomic DNA G+C content of strain UDC329(T) was 50.2 mol%. In phylogenetic analyses based on 16S rRNA and gyrB gene sequences, strain UDC329(T) was grouped with members of the genus Shewanella and appeared most closely related to Shewanella fodinae JC15(T) (97.9% 16S rRNA gene sequence similarity), Shewanella indica KJW27(T) (95.0%), Shewanella algae ATCC 51192(T) (94.8%), Shewanella haliotis DW01(T) (94.5%) and Shewanella chilikensis JC5(T) (93.9%). The level of DNA-DNA relatedness between strain UDC329(T) and S. fodinae JC15(T) was, however, only 27.4%. On the basis of phenotypic, genotypic and DNA-DNA relatedness data, strain UDC329(T) represents a novel species in the genus Shewanella, for which the name Shewanella dokdonensis sp. nov. is proposed. The type strain is UDC329(T) (=KCTC 22898(T)=DSM 23626(T)). PMID:21890724

  17. Pseudomonas punonensis sp. nov., isolated from straw.

    PubMed

    Ramos, Elena; Ramírez-Bahena, Martha-Helena; Valverde, Angel; Velázquez, Encarna; Zúñiga, Doris; Velezmoro, Carmen; Peix, Alvaro

    2013-05-01

    During a study of the 'tunta' (frozen-dry potato) production process in Peru, a bacterial strain, LMT03(T), was isolated from the straw grass in which the potatoes are dried. This strain was classified into the genus Pseudomonas on the basis of the 16S rRNA gene sequence analysis, and is most closely related to Pseudomonas argentinensis CH01(T) with 99.3?% identity in this gene and 96?%, 92?% and 86?% identities in rpoB, rpoD and gyrB genes, respectively. Strain LMT03(T) has a single polar flagellum, like other related yellow-pigment-producing pseudomonads. The major quinone is Q-9. The major fatty acids are C18?:?1?7c in summed feature 8 (40.82?%), C16?:?1?6c/C16?:?1?6c in summed feature 3 (23.72?%) and C16?:?0 (15.20?%). The strain produces oxidase but it does not produce gelatinase, indole, urease, arginine dihydrolase or ?-galactosidase. Catalase production was very weak after 28 and 48 h incubation on nutrient agar medium. Nitrate reduction is negative. It does not hydrolyse aesculin. The DNA G+C content is 57.8 mol%. DNA-DNA hybridization results showed lower than 52?% relatedness with respect to the type strain of P. argentinensis, CH01(T). These results, together with other phenotypic characteristics, support the definition of a novel species within the genus Pseudomonas, for which the name Pseudomonas punonensis sp. nov. is proposed. The type strain is LMT03(T) (?=?LMG 26839(T)?=?CECT 8089(T)). PMID:23002045

  18. Solimonas terrae sp. nov., isolated from soil.

    PubMed

    Kim, Soo-Jin; Moon, Ji-Young; Weon, Hang-Yeon; Ahn, Jae-Hyung; Chen, Wen-Ming; Kwon, Soon-Wo

    2014-04-01

    A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated strain KIS83-12(T), was isolated from soil of Gaui island in the Taean region of South Korea. The strain grew at 15-33 °C (optimum, 28 °C), at pH 5.0-8.0 (optimum, pH 7.0). Growth did not occur in the presence of NaCl. The strain was catalase-negative and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that KIS83-12(T) was most closely related to Solimonas soli DCY12(T) (96.9?%), Solimonas variicoloris MN28(T) (96.5?%), Solimonas flava CW-KD 4(T) (96.5?%) and Solimonas aquatica NAA16(T) (96.0?%), and formed a robust phyletic lineage with members of the genus Solimonas. The main isoprenoid quinone was Q-8. Major polar lipids included phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acids present in large and moderate amounts (>5.0?%) were summed feature 8 (C18?:?1?7c and/or C18?:?1?6c), C16?:?0, C16?:?1?5c, summed feature 2 (iso-C16?:?1 I and/or C14?:?0 3-OH) and C12?:?0. The DNA G+C content was 67.9 mol%. On the basis of the taxonomic data obtained in this study, KIS83-12(T) represents a novel species of the genus Solimonas, for which the name Solimonas terrae sp. nov. is proposed, with KIS83-12(T) (?=?KACC 16967(T)?=?DSM 27281(T)) as the type strain. PMID:24421276

  19. Streptomyces zagrosensis sp. nov., isolated from soil.

    PubMed

    Mohammadipanah, Fatemeh; Hamedi, Javad; Spröer, Cathrin; Rohde, Manfred; Montero-Calasanz, María del Carmen; Klenk, Hans-Peter

    2014-10-01

    The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus Streptomyces. On ISP2 medium, strain HM 1154(T) produced a dark cream, branched substrate mycelium and Retinaculiaperti aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 °C and pH 6-9 with 0-4% (w/v) NaCl. Whole-cell hydrolysates contained ll-diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H4) and MK-9(H2) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C16:0 and anteiso-C15:0. Strain HM 1154(T) exhibited the highest 16S rRNA gene sequence similarities to Streptomyces coerulescens DSM 40146(T) (99.4%), Streptomyces varsoviensis DSM 40346(T) (99.3%), Streptomyces youssoufiensis DSM 41920(T) (99.2%), Streptomyces abikoensis DSM 40831(T) (99.2%), Streptomyces rimosus subsp. rimosus DSM 40260(T) (99.1%), Streptomyces luteireticuli DSM 40509(T) (99.1%), Streptomyces thioluteus DSM 40027(T) (99.1%), Streptomyces blastmyceticus DSM 40029(T) (99.0%) and Streptomyces hiroshimensis DSM 40037(T) (99.0%). DNA-DNA hybridization studies showed relatedness values of 11.0-35.8% with the closest related species. Based on these results, strain HM 1154(T) is considered to represent a novel species within the genus Streptomyces, for which the name Streptomyces zagrosensis sp. nov. is proposed. The type strain is HM 1154(T) ( = DSM 42018(T) = UTMC 1154(T) = CECT 8305(T)). PMID:25030519

  20. Planomicrobium soli sp. nov., isolated from soil.

    PubMed

    Luo, Xiaonan; Zhang, Jianli; Li, Dai; Xin, Yuhua; Xin, Di; Fan, Lei

    2014-08-01

    A Gram-staining-positive bacterium, designated strain XN13(T), was isolated from a soil sample collected from ALaShan National Geological Park in Inner Mongolia Autonomous Region, China and subjected to a taxonomic study using a polyphasic approach. Strain XN13(T) was found to have a range of chemical and morphological properties consistent with its classification in the genus Planomicrobium. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain XN13(T) was related to members of the genus Planomicrobium. The closest phylogenetic relatives were Planomicrobium okeanokoites NBRC 12536(T), Planomicrobium koreense JG07(T), Planomicrobium mcmeekinii S23F2(T) and Planomicrobium flavidum ISL-41(T) with 98.2%, 97.8%, 97.8% and 97.7% 16S rRNA gene sequence similarity, respectively. The major cellular fatty acids were anteiso-C(15?:?0), C(16?:?1)?7c alcohol, iso-C(14?:?0) and C(16?:?1)?11c. The predominant menaquinones were MK-8 and MK-7. The DNA G+C content was 40.3 mol%. The DNA-DNA relatedness values between strain XN13(T) and Planomicrobium okeanokoites KCTC 3672(T), Planomicrobium koreense KCTC 3684(T), P. mcmeekinii CGMCC 1.2724(T), Planomicrobium flavidum KCTC 13261(T), Planomicrobium chinense CGMCC 1.3454(T) and Planomicrobium glaciei CGMCC 1.6846(T) were 36%, 30%, 34%, 29%, 30% and 31%, respectively. The organism is different from recognized species of the genus Planomicrobium in several phenotypic characteristics. On the basis of phenotypic and genotypic properties, strain XN13(T) represents a novel species of the genus Planomicrobium, for which the name Planomicrobium soli sp. nov. is proposed. The type strain is XN13(T) (?=?CGMCC 1.12259(T)?=?KCTC 33047(T)). PMID:24854007

  1. Systematic variation of the Raman spectra of DLC films as a function of sp 2:sp 3 composition

    Microsoft Academic Search

    S. Prawer; K. W. Nugent; Y. Lifshitz; G. D. Lempert; E. Grossman; J. Kulik; I. Avigal; R. Kalish

    1996-01-01

    We report the Raman spectra of a set of hydrogen-free diamond-like carbon (DLC) films prepared under conditions that result in different types of carbon bonding and for which the sp2:sp3 content was independently determined by electron energy loss spectroscopy. The films were prepared using a mass selected C+ ion beam deposition system covering the ion energy range 10 eV to

  2. Effects of cell growth and a mobile genetic element on propagation of the phages SP16 and SP-beta in Bacillus subtilis

    E-print Network

    England, Eleina M. (Eleina Helen Maria)

    2014-01-01

    Two studies were performed on Bacillus subtilis phages SP16 and SP-beta to characterize the effects of growth and an integrative conjugative element, ICEBs1, respectively. I found that B. subtilis strains are significantly ...

  3. On the fourth Diadema species (Diadema-sp) from Japan.

    PubMed

    Chow, Seinen; Kajigaya, Yoshikazu; Kurogi, Hiroaki; Niwa, Kentaro; Shibuno, Takuro; Nanami, Atsushi; Kiyomoto, Setuo

    2014-01-01

    Four long-spined sea urchin species in the genus Diadema are known to occur around the Japanese Archipelago. Three species (D. savignyi, D. setosum, and D. paucispinum) are widely distributed in the Indo-Pacific Ocean. The fourth species was detected by DNA analysis among samples originally collected as D. savignyi or D. setosum in Japan and the Marshall Islands and tentatively designated as Diadema-sp, remaining an undescribed species. We analyzed nucleotide sequences of the cytochrome oxidase I (COI) gene in the "D. savignyi-like" samples, and found all 17 individuals collected in the mainland of Japan (Sagami Bay and Kyushu) to be Diadema-sp, but all nine in the Ryukyu Archipelago (Okinawa and Ishigaki Islands) to be D. savignyi, with large nucleotide sequence difference between them (11.0%±1.7 SE). Diadema-sp and D. savignyi shared Y-shaped blue lines of iridophores along the interambulacrals, but individuals of Diadema-sp typically exhibited a conspicuous white streak at the fork of the Y-shaped blue iridophore lines, while this feature was absent in D. savignyi. Also, the central axis of the Y-shaped blue lines of iridophores was approximately twice as long as the V-component in D. savignyi whereas it was of similar length in Diadema-sp. Two parallel lines were observed to constitute the central axis of the Y-shaped blue lines in both species, but these were considerably narrower in Diadema-sp. Despite marked morphological and genetic differences, it appears that Diadema-sp has been mis-identified as D. savignyi for more than half a century. PMID:25054386

  4. 75 FR 42411 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Daily Contract; SP-15 Financial Day...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ...COMMISSION Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Daily Contract; SP-15 Financial Day-Ahead LMP Off-Peak Daily Contract; SP-15...LMP-Peak Daily Contract; NP-15 Financial Day- Ahead LMP Peak Daily Contract and...

  5. 75 FR 42380 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Contract and SP-15 Financial Day-Ahead...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ...COMMISSION Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Contract and SP-15 Financial Day-Ahead LMP Off-Peak Contract Offered for...determination whether the SP-15 \\2\\ Financial Day-Ahead LMP Peak (``SPM'') contract...

  6. Methylation of halogenated phenols and thiophenols by cell extracts of gram-positive and gram-negative bacteria. [Rhodococcus sp. ; Pseudomonas sp. ; Acinetobacter sp

    SciTech Connect

    Neilson, A.H.; Lindgren, C.; Hynning, P.A.; Remberger, M.

    1988-02-01

    O-methylation of 2,6-dibromophenol was studied in cell extracts prepared from Rhodococcus sp. strain 1395. O-methylation activity was also demonstrated in extracts from two other Rhodococcus sp. strains, an Acinetobacter sp. strain, and a Pseudomonas sp. strain. A diverse range of chloro- and bromophenols, chlorothiophenols, chloro- and bromoguaiacols, and chloro- and bromocatechols were assayed as the substrates by using extracts prepared from strain 1395; all of the compounds were methylated to the corresponding anisoles, veratroles, or guaiacols. The specific activity of the enzyme towards the thiophenols was significantly higher than it was towards all the other substrates-high activity was found with pentafluorothiophenol, although the activity with pentafluorophenol was undetectable with the incubation times used. For the chlorophenols, the position of the substituents was of cardinal importance. The enzyme had higher activity towards the halogenated catechols than towards the corresponding guaiacols, and selective O-methylation of the 3,4,5-trihalogenocatechols yielded predominantly the 3,4,5-trihalogenoguaiacols. Neither 2,4-dinitrophenol, hexachlorophene, nor 5-chloro- or 5-bromovanillin was O-methylated. The results showed conclusively that the methylation reactions were enzymatic and confirmed the conclusion from extensive studies using whole cells that methylation of halogenated phenols may be a significant alternative to biodegradation.

  7. Altererythrobacter aestiaquae sp. nov., isolated from seawater.

    PubMed

    Jung, Yong-Taek; Park, Sooyeon; Lee, Jung-Sook; Yoon, Jung-Hoon

    2014-12-01

    A Gram-stain-negative, coccoid- or oval-shaped, gliding bacterial strain, designated HDW-31(T), belonging to the class Alphaproteobacteria, was isolated from seawater of the Yellow Sea, Korea, and was subjected to a taxonomic study using a polyphasic approach. Strain HDW-31(T) grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3?% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HDW-31(T) fell within the clade comprising the genus Altererythrobacter, clustering with the type strains of Altererythrobacter luteolus and Altererythrobacter gangjinensis, with which strain HDW-31(T) exhibited 97.0 and 96.0?% sequence similarity values, respectively. Sequence similarities to the type strains of the other recognized species of the genus Altererythrobacter were 93.5-96.0?%. The DNA G+C content was 57.9 mol% and mean DNA-DNA relatedness between strain HDW-31(T) and the type strain of A. luteolus was 5.3?%. Strain HDW-31(T) contained Q-10 as the predominant ubiquinone and summed feature 8 (C18?:?1?7c and/or C18?:?1?6c), summed feature 3 (C16?:?1?6c and/or C16?:?1?7c) and C16?:?0 as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, a sphingoglycolipid, two unidentified glycolipids and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain HDW-31(T) is distinguishable from recognized species of the genus Altererythrobacter. On the basis of the data presented, strain HDW-31(T) is considered to represent a novel species of the genus Altererythrobacter, for which the name Altererythrobacter aestiaquae sp. nov. is proposed. The type strain is HDW-31(T) (?=?KCTC 42006(T)?=?CECT 8527(T)). PMID:25201916

  8. Enantiospecific sp2-sp3 coupling of secondary and tertiary boronic esters

    NASA Astrophysics Data System (ADS)

    Bonet, Amadeu; Odachowski, Marcin; Leonori, Daniele; Essafi, Stephanie; Aggarwal, Varinder K.

    2014-07-01

    The cross-coupling of boronic acids and related derivatives with sp2 electrophiles (the Suzuki-Miyaura reaction) is one of the most powerful C-C bond formation reactions in synthesis, with applications that span pharmaceuticals, agrochemicals and high-tech materials. Despite the breadth of its utility, the scope of this Nobel prize-winning reaction is rather limited when applied to aliphatic boronic esters. Primary organoboron reagents work well, but secondary and tertiary boronic esters do not (apart from a few specific and isolated examples). Through an alternative strategy, which does not involve using transition metals, we have discovered that enantioenriched secondary and tertiary boronic esters can be coupled to electron-rich aromatics with essentially complete enantiospecificity. As the enantioenriched boronic esters are easily accessible, this reaction should find considerable application, particularly in the pharmaceutical industry where there is growing awareness of the importance of, and greater clinical success in, creating biomolecules with three-dimensional architectures.

  9. Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A*

    PubMed Central

    Sever-Chroneos, Zvjezdana; Krupa, Agnieszka; Davis, Jeremy; Hasan, Misbah; Yang, Ching-Hui; Szeliga, Jacek; Herrmann, Mathias; Hussain, Muzafar; Geisbrecht, Brian V.; Kobzik, Lester; Chroneos, Zissis C.

    2011-01-01

    Staphylococcus aureus causes life-threatening pneumonia in hospitals and deadly superinfection during viral influenza. The current study investigated the role of surfactant protein A (SP-A) in opsonization and clearance of S. aureus. Previous studies showed that SP-A mediates phagocytosis via the SP-A receptor 210 (SP-R210). Here, we show that SP-R210 mediates binding and control of SP-A-opsonized S. aureus by macrophages. We determined that SP-A binds S. aureus through the extracellular adhesin Eap. Consequently, SP-A enhanced macrophage uptake of Eap-expressing (Eap+) but not Eap-deficient (Eap?) S. aureus. In a reciprocal fashion, SP-A failed to enhance uptake of Eap+ S. aureus in peritoneal Raw264.7 macrophages with a dominant negative mutation (SP-R210(DN)) blocking surface expression of SP-R210. Accordingly, WT mice cleared infection with Eap+ but succumbed to sublethal infection with Eap- S. aureus. However, SP-R210(DN) cells compensated by increasing non-opsonic phagocytosis of Eap+ S. aureus via the scavenger receptor scavenger receptor class A (SR-A), while non-opsonic uptake of Eap? S. aureus was impaired. Macrophages express two isoforms: SP-R210L and SP-R210S. The results show that WT alveolar macrophages are distinguished by expression of SP-R210L, whereas SR-A?/? alveolar macrophages are deficient in SP-R210L expressing only SP-R210S. Accordingly, SR-A?/? mice were highly susceptible to both Eap+ and Eap? S. aureus. The lungs of susceptible mice generated abnormal inflammatory responses that were associated with impaired killing and persistence of S. aureus infection in the lung. In conclusion, alveolar macrophage SP-R210L mediates recognition and killing of SP-A-opsonized S. aureus in vivo, coordinating inflammatory responses and resolution of S. aureus pneumonia through interaction with SR-A. PMID:21123169

  10. Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov., isolated from the Russian space laboratory Mir.

    PubMed

    Li, Ying; Kawamura, Yoshiaki; Fujiwara, Nagatoshi; Naka, Takashi; Liu, Hongsheng; Huang, Xinxiang; Kobayashi, Kazuo; Ezaki, Takayuki

    2004-05-01

    On the basis of phenotypic and genotypic characteristics and 16S rRNA gene sequence analysis, novel species belonging to the genera Sphingomonas and Brevundimonas were identified from samples taken from the Russian space laboratory Mir. Strain A1-18(T) was isolated from the air. 16S rDNA sequence analysis showed that strain A1-18(T) formed a coherent cluster with Sphingomonas sanguinis, Sphingomonas parapaucimobilis, Sphingomonas paucimobilis and Sphingomonas roseiflava with sequence similarity of 97.5-98.6 %. Similar to other Sphingomonas species, the G+C content was 66.1 mol%, but DNA-DNA hybridization rates at optimal temperatures among these related species were only 24.7-51.7 %. Strain A1-18(T) can be differentiated biochemically from related species. Strain W1-2B(T) was isolated from condensation water. It forms a distinct lineage within the genus Brevundimonas, forming a coherent cluster with Brevundimonas vesicularis, Brevundimonas aurantiaca and Brevundimonas intermedia. 16S rDNA sequence similarities were 98.6-99.5 % and the G+C content was 66.5 mol%, similar to other Brevundimonas species, but DNA-DNA relatedness was only 50.2-54.8 %. Strain W1-2B(T) also showed some differential biochemical properties from its related species. A series of polyphasic taxonomic studies led to the proposal of two novel species, Sphingomonas yabuuchiae sp. nov. (type strain A1-18(T)=GTC 868(T)=JCM 11416(T)=DSM 14562(T)) and Brevundimonas nasdae sp. nov. (type strain W1-2B(T)=GTC 1043(T)=JCM 11415(T)=DSM 14572(T)). PMID:15143030

  11. Glycomyces fuscus sp. nov. and Glycomyces albus sp. nov., actinomycetes isolated from a hypersaline habitat.

    PubMed

    Han, Xiao-Xue; Luo, Xiao-Xia; Zhang, Li-Li

    2014-07-01

    Two actinomycete strains, designated TRM 49117(T) and TRM 49136(T), were isolated from a hypersaline habitat in Xinjiang Province, north-west China and were characterized taxonomically by using a polyphasic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain TRM 49117(T) had 93.93% similarity with the type strain Glycomyces halotolerans TRM 40137(T) (GenBank accession no. HQ651156) and TRM 49136(T) had 94.32% similarity with G. halotolerans TRM 40137(T). The 16S rRNA gene sequence similarity between the two new isolates was 93%. The isolates contained meso-diaminopimelic acid as the diagnostic diamino acid and anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 as major cellular fatty acids. The predominant menaquinones of the isolates were MK-9(H4) and MK-9(H6). The whole-cell sugar patterns of these strains contained xylose and ribose, and strain TRM 49136(T) also contained arabinose. The polar lipid pattern of strain TRM 49117(T) comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylcholine, phosphatidylinositol and three additional unknown phospholipids. The polar lipid pattern of strain TRM 49136(T) comprised phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, glycolipids and two phosphoglycolipids of unknown composition. Genotypic and phenotypic data confirmed that strains TRM 49117(T) and TRM 49136(T) represent two novel species, clearly different from related species of the genus Glycomyces, for which the names Glycomyces fuscus sp. nov. (type strain TRM 49117(T)?= CCTCC AA 2013003(T)?= NRRL B-59998(T)?= KACC 17682(T)) and Glycomyces albus sp. nov. (type strain TRM 49136(T)?= CCTCC AA 2013004(T)?= NRRL B-24927(T)?= KACC 17681(T)) are proposed. PMID:24776532

  12. Methylobacterium haplocladii sp. nov. and Methylobacterium brachythecii sp. nov., isolated from bryophytes.

    PubMed

    Tani, Akio; Sahin, Nurettin

    2013-09-01

    Pink-pigmented, facultatively methylotrophic bacteria, strains 87e(T) and 99b(T), were isolated from the bryophytes Haplocladium microphyllum and Brachythecium plumosum, respectively. The cells of both strains were Gram-reaction-negative, motile, non-spore-forming rods. On the basis of 16S rRNA gene sequence similarity, strains 87e(T) and 99b(T) were found to be related to Methylobacterium organophilum ATCC 27886(T) (97.1% and 97.7%, respectively). Strains 87e(T) and 99b(T) showed highest 16S rRNA gene similarity to Methylobacterium gnaphalii 23e(T) (98.3 and 99.0%, respectively). The phylogenetic similarities to all other species of the genus Methylobacterium with validly published names were less than 97%. Major cellular fatty acids of both strains were C(18:1)?7c and C(18:0). The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrix-assisted, laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains 87e(T) and 99b(T) from their phylogenetically closest relatives. We propose that strains 87e(T) and 99b(T) represent novel species within the genus Methylobacterium, for which the names Methylobacterium haplocladii sp. nov. (type strain 87e(T)?=DSM 24195(T)?=NBRC 107714(T)) and Methylobacterium brachythecii sp. nov. (type strain 99b(T)?=DSM 24105(T)?=NBRC 107710(T)) are proposed. PMID:23475343

  13. Bacteroides stercorirosoris sp. nov. and Bacteroides faecichinchillae sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces.

    PubMed

    Kitahara, Maki; Sakamoto, Mitsuo; Tsuchida, Sayaka; Kawasumi, Koh; Amao, Hiromi; Benno, Yoshimi; Ohkuma, Moriya

    2012-05-01

    Strains of gram-negative anaerobic rods were isolated from chinchilla (Chinchilla lanigera) faeces, and three strains, ST161(T), ST33 and ST37(T), were investigated taxonomically. Based on phylogenetic analyses and specific phenotypic characteristics, the three strains were allocated to the genus Bacteroides. Phylogenetic analyses of their 16S rRNA gene sequences revealed that strain ST161(T) formed a distinct line of descent, with highest sequence similarity to strain ST33 (98.7?%) and Bacteroides oleiciplenus JCM 16102(T) (97.7?%). High levels of DNA-DNA relatedness (79-89?%) were found between strains ST161(T) and ST33, but low levels were found between strain ST161(T) and B. oleiciplenus JCM 16102(T) (33-37?%) and between strain ST33 and B. oleiciplenus JCM 16102(T) (33-37?%). These data clearly indicated that strains ST161(T) and ST33 represent a single novel species. 16S rRNA gene sequence analyses showed that strain ST37(T) also formed a distinct line of descent, with highest sequence similarity to Bacteroides acidifaciens JCM 10556(T) (96.5?%) and Bacteroides caccae JCM 9498(T) (95.6?%). Analysis of hsp60 gene sequences also supported these relationships. Based on phenotypic and phylogenetic characteristics, two novel species, Bacteroides stercorirosoris sp. nov. and Bacteroides faecichinchillae sp. nov., are thus proposed. The type strains of B. stercorirosoris and B. faecichinchillae are ST161(T) (?=?JCM 17103(T)?=?CCUG 60872(T)) and ST37(T) (?=?JCM 17102(T)?=?CCUG 60873(T)), respectively. The DNA G+C contents of strains ST161(T) and ST37(T) were 45.7 and 41.0 mol%, respectively. PMID:21724955

  14. Nonomuraea guangzhouensis sp. nov., and Nonomuraea harbinensis sp. nov., two novel actinomycetes isolated from soil.

    PubMed

    Wang, Shurui; Liu, Chongxi; Zhang, Yuejing; Zhao, Junwei; Zhang, Xinhui; Yang, Lingyu; Wang, Xiangjing; Xiang, Wensheng

    2014-01-01

    Two novel actinomycetes, designated strains NEAU-ZJ3(T) and NEAU-yn31(T), were isolated from soils in China and their taxonomic positions determined by a polyphasic approach. Key morphological and chemotaxonomic properties of the two strains were consistent with the description of the genus Nonomuraea. The G+C contents of strains NEAU-ZJ3(T) and NEAU-yn31(T) were determined to be 67.4 and 68.0 %, respectively. Phylogenetic analysis using 16S rRNA gene sequences indicated that the two strains should be classified in the genus Nonomuraea. The 16S rRNA gene sequence of strain NEAU-ZJ3(T) showed it to be most closely related to Nonomuraea salmonea DSM 43678(T) (98.27 %), Nonomuraea endophytica YIM 65601(T) (98.23 %) and Nonomuraea candida HMC10(T) (98.08 %), and that of strain NEAU-yn31(T) to Nonomuraea ferruginea IFO 14094(T) (99.08 %) and N. candida HMC10(T) (98.01 %). Furthermore, a combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that the two strains can be distinguished from each other and their phylogenetically closest relatives. Thus, strains NEAU-ZJ3(T) and NEAU-yn31(T) represent two novel species of the genus Nonomuraea, for which the names Nonomuraea guangzhouensis sp. nov. and Nonomuraea harbinensis sp. nov. are proposed. The type strains are NEAU-ZJ3(T) (=CGMCC 4.7101(T) = DSM 45889(T)) and NEAU-yn31(T) (=CGMCC 4.7106(T) = DSM 45887(T)), respectively. PMID:24179038

  15. Hymenobacter koreensis sp. nov. and Hymenobacter saemangeumensis sp. nov., isolated from estuarine water.

    PubMed

    Kang, Ji Young; Chun, Jeesun; Choi, Ahyoung; Moon, Sung Hyun; Cho, Jang-Cheon; Jahng, Kwang Yeop

    2013-12-01

    Two Gram-reaction-negative, rod-shaped, non-motile and red-pink-pigmented bacterial strains, designated GYR3077(T) and GSR0100(T), were isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do, South Korea, and were characterized using a polyphasic approach. 16S rRNA genes of strains GYR3077(T) and GSR0100(T) exhibited sequence similarities of 95.9?% to Hymenobacter deserti ZLB-3(T) and 96.6?% to Hymenobacter soli PB17(T), respectively, and indicated that these isolates belonged to the phylum Bacteroidetes. The major cellular fatty acids present in the two isolates were iso-C15?:?0, C16?:?1?5c, summed feature 4 (iso-C17?:?1 I and/or anteiso-C17?:?1 B) and summed feature 3 (C16?:?1?7c and/or C16?:?1?6c). The major respiratory quinone and polyamine patterns were menaquinone-7 and sym-homospermidine, characteristic of the genus Hymenobacter. Flexirubin-type pigments were absent in both strains. The DNA G+C contents of strains GYR3077(T) and GSR0100(T) were 60.2 mol% and 61.9 mol%, respectively. The major polar lipid of strains GYR3077(T) and GSR0100(T) was phosphatidylethanolamine. Based on the morphological and physiological properties, strains GYR3077(T) and GSR0100(T) were considered to represent two novel species of the genus Hymenobacter, for which the names Hymenobacter koreensis sp. nov. (type strain GYR3077(T)?=?KACC 16451(T)?=?JCM 17924(T)) and Hymenobacter saemangeumensis sp. nov. (type strain GSR0100(T)?=?KACC 16452(T)?=?JCM 17923(T)) are proposed. PMID:23907229

  16. Sp3 regulates fas expression in lung epithelial cells.

    PubMed Central

    Pang, H; Miranda, K; Fine, A

    1998-01-01

    By transducing an apoptotic signal in immune effector cells, Fas has been directly implicated in the control of immunological activity. Expression and functional results, however, have also suggested a role for Fas in regulating cell turnover in specific epithelial populations. To characterize factors responsible for Fas expression in epithelial cells, approximately 3 kb of the 5' flanking region of the mouse Fas gene was isolated. By rapid amplification of cDNA ends and primer extension, transcriptional start sites were identified within 50 bp upstream of the translation start site. Transient transfection of promoter-luciferase constructs in a mouse lung epithelial cell line, MLE-15, localized promoter activity to the first 77 bp of upstream sequence. By using a 60 bp DNA probe (-18 to -77) in electrophoretic mobility-shift assays, three shifted complexes were found. Incubation with excess cold Sp1 oligonucleotide or an anti-Sp3 antibody inhibited complex formation. Site-directed mutagenesis of the Sp1 site resulted in 60-70% loss of promoter activity. In Drosophila SL-2 cells, promoter activity was markedly increased by co-transfection of an Sp3 expression construct. These results show that the Sp3 protein is involved in regulating Fas gene expression in lung epithelial cells. PMID:9639581

  17. Methods for the genetic manipulation of Nonomuraea sp. ATCC 39727.

    PubMed

    Marcone, Giorgia Letizia; Foulston, Lucy; Binda, Elisa; Marinelli, Flavia; Bibb, Mervyn; Beltrametti, Fabrizio

    2010-10-01

    Nonomuraea sp. ATCC 39727 belongs to the Streptosporangiaceae family of filamentous actinomycetes. This microorganism produces the teicoplanin-like glycopeptide A40926, which is the starting material for the synthesis of the second-generation glycopeptide dalbavancin. Notwithstanding the strain's pharmaceutical relevance, the lack or poor efficiency of genetic tools to manipulate Nonomuraea sp. ATCC 39727 has hampered strain and product improvement. Here we report the development of gene transfer systems based on protoplast transformation and intergeneric conjugation from Escherichia coli. Efficiency of transformation and conjugation, followed by site specific or homologous recombination with the Nonomuraea sp. genome, were determined using the integrative plasmid pSET152 (5.7 kb), and the Supercos1 derivative cosmid A40?Y (30 kb). To our knowledge, this is the first report of the transformation of protoplasts of Nonomuraea sp. ATCC 39727, even though the improved procedure for intergeneric conjugation makes it the method of choice for introducing large segments of DNA into Nonomuraea sp. ATCC 39727. PMID:20717702

  18. Reversibility of pulmonary abnormalities by conditional replacement of surfactant protein D (SP-D) in vivo.

    PubMed

    Zhang, Liqian; Ikegami, Machiko; Dey, Chitta R; Korfhagen, Thomas R; Whitsett, Jeffrey A

    2002-10-11

    Surfactant protein D (SP-D) gene-targeted mice develop severe pulmonary disease associated with emphysema, pulmonary lipidosis, and foamy macrophage infiltrations. To determine the potential reversibility of these abnormalities, transgenic mice were developed in which SP-D was conditionally replaced in the respiratory epithelium of SP-D(-/-) mice. SP-D was not detected in the absence of doxycycline. Treatment with doxycycline after birth restored pulmonary SP-D concentrations and corrected pulmonary pathology at adulthood. When SP-D was replaced in adult SP-D(-/-) mice, alveolar SP-D was restored within 3 days, pulmonary lipid abnormalities were corrected, but emphysema persisted. In corrected adult SP-D(-/-) mice, loss of SP-D caused focal emphysema and pulmonary inflammation but did not cause phospholipid abnormalities characteristic of SP-D(-/-) mice. Thus, abnormalities in surfactant phospholipid homeostasis and alveolar macrophage abnormalities were readily corrected by restoration of SP-D. However, once established, emphysema was not reversed by SP-D. SP-D-dependent processes regulating surfactant lipid homeostasis were disassociated from those mediating emphysema. PMID:12163500

  19. Sp1 transcription factor: A long-standing target in cancer chemotherapy.

    PubMed

    Vizcaíno, Carolina; Mansilla, Sylvia; Portugal, José

    2015-08-01

    Sp1 (specificity protein 1) is a well-known member of a family of transcription factors that also includes Sp2, Sp3 and Sp4, which are implicated in an ample variety of essential biological processes and have been proven important in cell growth, differentiation, apoptosis and carcinogenesis. Sp1 activates the transcription of many cellular genes that contain putative CG-rich Sp-binding sites in their promoters. Sp1 and Sp3 proteins bind to similar, if not the same, DNA tracts and compete for binding, thus they can enhance or repress gene expression. Evidences exist that the Sp-family of proteins regulates the expression of genes that play pivotal roles in cell proliferation and metastasis of various tumors. In patients with a variety of cancers, high levels of Sp1 protein are considered a negative prognostic factor. A plethora of compounds can interfere with the trans-activating activities of Sp1 and other Sp proteins on gene expression. Several pathways are involved in the down-regulation of Sp proteins by compounds with different mechanisms of action, which include not only the direct interference with the binding of Sp proteins to their putative DNA binding sites, but also promoting the degradation of Sp protein factors. Down-regulation of Sp transcription factors and Sp1-regulated genes is drug-dependent and it is determined by the cell context. The acknowledgment that several of those compounds are safe enough might accelerate their introduction into clinical usage in patients with tumors that over-express Sp1. PMID:25960131

  20. SP-R210 (Myo18A) Isoforms as Intrinsic Modulators of Macrophage Priming and Activation

    PubMed Central

    DiAngelo, Susan L.; Silveyra, Patricia; Umstead, Todd M.; Halstead, E. Scott; Davies, Michael L.; Hu, Sanmei; Floros, Joanna; McCormack, Francis X.; Christensen, Neil D.; Chroneos, Zissis C.

    2015-01-01

    The surfactant protein (SP-A) receptor SP-R210 has been shown to increase phagocytosis of SP-A-bound pathogens and to modulate cytokine secretion by immune cells. SP-A plays an important role in pulmonary immunity by enhancing opsonization and clearance of pathogens and by modulating macrophage inflammatory responses. Alternative splicing of the Myo18A gene results in two isoforms: SP-R210S and SP-R210L, with the latter predominantly expressed in alveolar macrophages. In this study we show that SP-A is required for optimal expression of SP-R210L on alveolar macrophages. Interestingly, pre-treatment with SP-A prepared by different methods either enhances or suppresses responsiveness to LPS, possibly due to differential co-isolation of SP-B or other proteins. We also report that dominant negative disruption of SP-R210L augments expression of receptors including SR-A, CD14, and CD36, and enhances macrophages’ inflammatory response to TLR stimulation. Finally, because SP-A is known to modulate CD14, we used a variety of techniques to investigate how SP-R210 mediates the effect of SP-A on CD14. These studies revealed a novel physical association between SP-R210S, CD14, and SR-A leading to an enhanced response to LPS, and found that SP-R210L and SP-R210S regulate internalization of CD14 via distinct macropinocytosis-like mechanisms. Together, our findings support a model in which SP-R210 isoforms differentially regulate trafficking, expression, and activation of innate immune receptors on macrophages. PMID:25965346

  1. Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09*

    PubMed Central

    Chen, Yun-mei; Xu, Hai-yang; Wang, Yang; Zhang, Jian-fa; Wang, Shi-ming

    2014-01-01

    Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity. PMID:25367790

  2. Prevalence of Brucella sp. antibodies in feral swine in Florida.

    PubMed

    van der Leek, M L; Becker, H N; Humphrey, P; Adams, C L; Belden, R C; Frankenberger, W B; Nicoletti, P L

    1993-07-01

    Serum samples collected from feral swine (Sus scrofa) throughout Florida (USA) from 1974 to 1989 were tested for antibodies to Brucella sp. by the card test, the standard tube test, the rivanol test or the complement fixation test. Seropositive swine were detected at six of 18 sites with a composite prevalence of 23.4% (238 of 1,015 samples; range = 5.5% to 33.3%) for sites with seropositive swine. At one site for which age and sex data were available there was no significant difference (P = 0.50) in seroprevalence between males and females. Antibody prevalence in adult (> or = 8 mo) and juvenile swine (< 8 mo), however, was significantly different (P < 0.05). Based on these data, Brucella sp. infections are limited only to certain populations of feral swine. To avoid the spread of Brucella sp. organisms, however, relocation of feral swine is not recommended. PMID:8355342

  3. Biochemical changes induced by fungicides in nitrogen fixing Nostoc sp.

    PubMed

    Deviram, G V N S; Pant, Gaurav; Prasuna, R Gyana

    2013-01-01

    The present study indicates the effect of fungicides (approved by WHO) and their behavior on nitrogen fixer of rice eco system Nostoc sp. Application of plant protecting chemicals at recommended levels braced up the growth of blue green algae thereby enhancing heterocyst formation and nitrogenase activity. Nostoc sp demoed varying degrees of sensitivity to fungicides. Biomass yield, protein, carbohydrate content reduced after 3pg/mL concentration. Heterocyst damage was observed from 4?g/mL, Proline content increased with increase in fungicide concentration, utmost yellowing of the culture started from 4?g/mL. The decreasing order of the toxicity to Nostoc sp with fungicides was Mancozeb> Ediphenphos> Carbendazim> Hexaconazole. PMID:25464710

  4. Biochemical changes induced by fungicides in nitrogen fixing Nostoc sp.

    PubMed

    Deviram, G V N S; Pant, Gaurav; Prasuna, R Gyana

    2013-01-01

    The present study indicates the effect of fungicides (approved by WHO) and their behavior on nitrogen fixer of rice eco system Nostoc sp. Application of plant protecting chemicals at recommended levels braced up the growth of blue green algae thereby enhancing heterocyst formation and nitrogenase activity. Nostoc sp demoed varying degrees of sensitivity to fungicides. Biomass yield, protein, carbohydrate content reduced after 3pg/mL concentration. Heterocyst damage was observed from 4?g/mL, Proline content increased with increase in fungicide concentration, utmost yellowing of the culture started from 4?g/mL. The decreasing order of the toxicity to Nostoc sp with fungicides was Mancozeb> Ediphenphos> Carbendazim> Hexaconazole. PMID:25508341

  5. Polymorphic phases of sp3-hybridized carbon under cold compression.

    PubMed

    Zhou, Rulong; Zeng, Xiao Cheng

    2012-05-01

    It is well established that graphite can be transformed into superhard carbons under cold compression (Mao et al. Science 2003, 302, 425). However, structure of the superhard carbon is yet to be determined experimentally. We have performed an extensive structural search for the high-pressure crystalline phases of carbon using the evolutionary algorithm. Nine low-energy polymorphic structures of sp(3)-hybridized carbon result from the unbiased search. These new polymorphic carbon structures together with previously reported low-energy sp(3)-hybridized carbon structures (e.g., M-carbon, W-carbon, and Cco-C(8) or Z-carbon) can be classified into three groups on the basis of different ways of stacking two (or more) out of five (A-E) types of buckled graphene layers. Such a classification scheme points out a simple way to construct a variety of sp(3)-hybridized carbon allotropes via stacking buckled graphene layers in different combinations of the A-E types by design. Density-functional theory calculations indicate that, among the nine low-energy crystalline structures, seven are energetically more favorable than the previously reported most stable crystalline structure (i.e., Cco-C(8) or Z-carbon) in the pressure range 0-25 GPa. Moreover, several newly predicted polymorphic sp(3)-hybridized carbon structures possess elastic moduli and hardness close to those of the cubic diamond. In particular, Z-carbon-4 possesses the highest hardness (93.4) among all the low-energy sp(3)-hybridized carbon structures predicted today. The calculated electronic structures suggest that most polymorphic carbon structures are optically transparent. The simulated X-ray diffraction (XRD) spectra of a few polymorphic structures are in good agreement with the experimental spectrum, suggesting that samples from the cold-compressed graphite experiments may consist of multiple polymorphic phases of sp(3)-hybridized carbon. PMID:22490007

  6. Methylomonas sp. strain 761M: an unusual Type I methanotroph

    SciTech Connect

    Zhao, S.J.; Hanson, R.S.

    1985-01-01

    Methylomonas sp. strain 761M and strains derived from it are described as unusual type I methanotrophs that require multicarbon compounds for rapid growth. Alpha-ketoglutarate dehydrogenase is present in these strains, and they contain a functional tricarboxylic acid cycle. Methylomonas sp. strain 761M appears to be well-adapted to studies of energy and growth yields on C/sub 1/ substrates because the assimilation of C/sub 1/ units can be substantially reduced. 16 references, 2 figures, 4 tables.

  7. Fuels irradiation testing for the SP-100 program

    NASA Astrophysics Data System (ADS)

    Makenas, Bruce J.; Hales, Janell W.; Ward, Alva L.

    An SP-100 fuel pin irradiation testing program is well on the way to providing data for performance correlations and demonstrating the lifetime and safety of the fuel system of the compact lithium-cooled reactor. Key SP-100 fuel performance issues addressed are the need for low fuel swelling and low fission gas release to minimize cladding strain, and the need for barrier integrity to prevent fuel/cladding chemical interaction. This paper provides a description of the irradiation test program that addresses these key issues and summarizes recent results of posttest examinations including data obtained at 6 atom percent goal burnup.

  8. Fuels irradiation testing for the SP-100 program

    NASA Technical Reports Server (NTRS)

    Makenas, Bruce J.; Hales, Janell W.; Ward, Alva L.

    1991-01-01

    An SP-100 fuel pin irradiation testing program is well on the way to providing data for performance correlations and demonstrating the lifetime and safety of the fuel system of the compact lithium-cooled reactor. Key SP-100 fuel performance issues addressed are the need for low fuel swelling and low fission gas release to minimize cladding strain, and the need for barrier integrity to prevent fuel/cladding chemical interaction. This paper provides a description of the irradiation test program that addresses these key issues and summarizes recent results of posttest examinations including data obtained at 6 atom percent goal burnup.

  9. Development status of the SP-100 power system

    NASA Technical Reports Server (NTRS)

    Mondt, Jack F.

    1989-01-01

    The SP-100 Program, whose goal is to develop 10 to 1000 kW electric space reactor power systems for use in civil and military space missions in the mid 1990s and beyond, is described. The major accomplishments of the SP-100 ground engineering system development project are: (1) completion of the preliminary design of the reactor test facility, and (2) completion of the generic flight system design which meets the technical specifications and is the basis for all the ground engineering system analytical and experimental tasks. The power conditioning, control, and distribution subsystem is indicated schematically.

  10. Cotoneaster sp. poisoning in a llama (Lama glama).

    PubMed

    Grüss, Aurélie; Priymenko, Nathalie

    2009-03-01

    A case of poisoning with a Cotoneaster sp. is described in a llama (Lama glama). The clinical signs were dyspnea, with congested mucous membranes, chewing, recumbency, and opisthotonos. Several hours after ingestion of the plant, the llama died, despite treatment with sodium thiosulfate and nitrite. Necropsy revealed approximately 676 g of leaves and fruit of Cotoneaster in the first compartment of the stomach. The blood was light red and did not clot. Because Cotoneaster sp. contains low concentrations of cyanogenic glycosides, toxicosis occurs only following massive ingestion of plant material. PMID:19286507

  11. Regulation of Histone Deacetylase 4 Expression by the SP Family of Transcription FactorsD?

    PubMed Central

    Liu, Fang; Pore, Nabendu; Kim, Mijin; Voong, K. Ranh; Dowling, Melissa; Maity, Amit; Kao, Gary D.

    2006-01-01

    Histone deacetylases mediate critical cellular functions but relatively little is known about mechanisms controlling their expression, including expression of HDAC4, a class II HDAC implicated in the modulation of cellular differentiation and viability. Endogenous HDAC4 mRNA, protein levels and promoter activity were all readily repressed by mithramycin, suggesting regulation by GC-rich DNA sequences. We validated consensus binding sites for Sp1/Sp3 transcription factors in the HDAC4 promoter through truncation studies and targeted mutagenesis. Specific and functional binding by Sp1/Sp3 at these sites was confirmed with chromatin immunoprecipitation (ChIP) and electromobility shift assays (EMSA). Cotransfection of either Sp1 or Sp3 with a reporter driven by the HDAC4 promoter led to high activities in SL2 insect cells (which lack endogenous Sp1/Sp3). In human cells, restored expression of Sp1 and Sp3 up-regulated HDAC4 protein levels, whereas levels were decreased by RNA-interference-mediated knockdown of either protein. Finally, variable levels of Sp1 were in concordance with that of HDAC4 in a number of human tissues and cancer cell lines. These studies together characterize for the first time the activity of the HDAC4 promoter, through which Sp1 and Sp3 modulates expression of HDAC4 and which may contribute to tissue or cell-line-specific expression of HDAC4. PMID:16280357

  12. Transcription factor Sp3 is silenced through SUMO modification by PIAS1

    PubMed Central

    Sapetschnig, Alexandra; Rischitor, Grigore; Braun, Harald; Doll, Andreas; Schergaut, Marion; Melchior, Frauke; Suske, Guntram

    2002-01-01

    Sp3 is a ubiquitous transcription factor closely related to Sp1. Here we show that Sp3 is a target for SUMO modification in vivo and in vitro. SUMO modification of Sp3 occurs at a single lysine located between the second glutamine-rich activation domain and the DNA-binding domain. Mutational analyses identified the sequence IKXE as essential for SUMO conjugation to Sp3. We identified the protein inhibitor of activated STAT1 (PIAS1) as an interaction partner of Sp3 and Ubc9. Moreover, PIAS1 strongly stimulated SUMO conjugation to Sp3, thus acting as an E3 ligase for SUMO conjugation to Sp3. All mutations that prevented SUMO modification in vitro strongly enhanced the transcriptional activity of Sp3, showing that SUMO modification silences Sp3 activity. SUMO-modified Sp3 bound to DNA with similar specificity and affinity as unmodified Sp3. However, DNA-bound Sp3 did not act as a substrate for SUMO modification. PMID:12356736

  13. Fusarium euwallaceae sp. nov.--a symbiotic fungus of Euwallacea sp., an invasive ambrosia beetle in Israel and California.

    PubMed

    Freeman, S; Sharon, M; Maymon, M; Mendel, Z; Protasov, A; Aoki, T; Eskalen, A; O'Donnell, K

    2013-01-01

    The invasive Asian ambrosia beetle Euwallacea sp. (Coleoptera, Scolytinae, Xyleborini) and a novel Fusarium sp. that it farms in its galleries as a source of nutrition causes serious damage to more than 20 species of live trees and pose a serious threat to avocado production (Persea americana) in Israel and California. Adult female beetles are equipped with mandibular mycangia in which its fungal symbiont is transported within and from the natal galleries. Damage caused to the xylem is associated with disease symptoms that include sugar or gum exudates, dieback, wilt and ultimately host tree mortality. In 2012 the beetle was recorded on more than 200 and 20 different urban landscape species in southern California and Israel respectively. Euwallacea sp. and its symbiont are closely related to the tea shot-hole borer (E. fornicatus) and its obligate symbiont, F. ambrosium occurring in Sri Lanka and India. To distinguish these beetles, hereafter the unnamed xyleborine in Israel and California will be referred to as Euwallacea sp. IS/CA. Both fusaria exhibit distinctive ecologies and produce clavate macroconidia, which we think might represent an adaption to the species-specific beetle partner. Both fusaria comprise a genealogically exclusive lineage within Clade 3 of the Fusarium solani species complex (FSSC) that can be differentiated with arbitrarily primed PCR. Currently these fusaria can be distinguished only phenotypically by the abundant production of blue to brownish macroconidia in the symbiont of Euwallacea sp. IS/CA and their rarity or absence in F. ambrosium. We speculate that obligate symbiosis of Euwallacea and Fusarium, might have driven ecological speciation in these mutualists. Thus, the purpose of this paper is to describe and illustrate the novel, economically destructive avocado pathogen as Fusarium euwallaceae sp. nov. S. Freeman et al. PMID:23928415

  14. Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae).

    PubMed

    Gupta, Arvind Kumar; Dharne, Mahesh Shantappa; Rangrez, Ashraf Yusuf; Verma, Pankaj; Ghate, Hemant V; Rohde, Manfred; Patole, Milind Shivaji; Shouche, Yogesh Shreepad

    2011-06-01

    Two Gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains FFA1(T) and FFA3(T), and belonging to the class Gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (Diptera: Sarcophagidae). Phylogenetic analysis of 16S rRNA gene sequence data placed these two strains within the genus Ignatzschineria with similarities of 98.6 % (FFA1(T)) and 99.35 % (FFA3(T)) to Ignatzschineria larvae L1/68(T). The level of gene sequence similarity between strains FFA1(T) and FFA3(T) was 99 %, 97.15 % and 78.1 % based on the 16S rRNA, 23S rRNA and gyrB gene sequences, respectively. Strains FFA1(T) and FFA3(T) shared 24 % DNA-DNA relatedness. DNA-DNA hybridization revealed a very low level of relatedness between the novel strains (22?% for strain FFA1(T) and 44 % for strain FFA3(T)) and I. larvae L1/68(T) genomic DNA. The respiratory quinone was Q-8 in both novel strains. The DNA G+C contents were 41.1 mol% and 40.1 mol% for strains FFA1(T) and FFA3(T), respectively. The cell membrane of both strains consisted of phosphatidylglycerol, phosphatidylethanolamine, phospholipids and aminophospholipid. The major fatty acids for both strains were C(16 : 0), summed feature 8 (C(18 : 1)?7c and/or C(18 : 1)?6c), CyC(19 : 0)?8c and C(14 : 0). The results of DNA-DNA hybridization between the two new strains and I. larvae L1/68(T), in combination with phylogenetic, chemotaxonomic, biochemical and electron microscopic data, demonstrated that strains FFA1(T) and FFA3(T) represented two novel species of the genus Ignatzschineria for which the names Ignatzschineria indica sp. nov. (type strain FFA1(T)?=?DSM 22309(T)?=?KCTC 22643(T)?=?NCIM 5325(T)) and Ignatzschineria ureiclastica sp. nov. (type strain FFA3(T)?=?DSM 22310(T)?=?KCTC 22644(T)?=?NCIM 5326(T)) are proposed. PMID:20584814

  15. Actinomadura amylolytica sp. nov. and Actinomadura cellulosilytica sp. nov., isolated from geothermally heated soil.

    PubMed

    Jiao, Jian-Yu; Liu, Lan; Zhou, En-Min; Wei, Da-Qiao; Ming, Hong; Xian, Wen-Dong; Yuan, Chang-Guo; Zhong, Jing-Mei; Li, Wen-Jun

    2015-07-01

    Two aerobic, Gram-positive actinomycetes, designated YIM 77502(T) and YIM 77510(T), were isolated from geothermally heated soil of Tengchong county, Yunnan province, south-west China. The taxonomic position of strains YIM 77502(T) and YIM 77510(T) were investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains YIM 77502(T) and YIM 77510(T) belong to the genus Actinomadura. Both strains form extensively-branched substrate and aerial mycelia which differentiated into short spore chains. The cell wall of the two strains contained meso-diaminopimelic acid, while the whole-cell sugars detected were glucose, madurose, mannose and rhamnose. The polar lipid profile of strain YIM 77502(T) was found to consist of diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, two unidentified phospholipids and an unidentified polar lipid, while strain YIM 77510(T) consisted of diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The respiratory quinones of strains YIM 77502(T) and YIM 77510(T) were MK-9(H6) and MK-9(H8). The major fatty acids (>10 %) of strain YIM 77502(T) were C17:0, iso-C16:0, C17:010-methyl and iso-C18:0, and those of strain YIM 77510(T) were iso-C16:0, C17:010-methyl and iso-C18:0. The G+C contents of strains YIM 77502(T) and YIM 77510(T) were determined to be 71.3 and 70.2 mol%, respectively. The DNA-DNA hybridization values of strains YIM 77502(T), YIM 77510(T) and their closest phylogenetic neighbours Actinomadura echinospora BCRC 12547(T) and Actinomadura umbrina KCTC 9343(T) were less than 70 %. Based on the morphological and physiological properties, and phylogenetic analyses, strains YIM 77502(T) and YIM 77510(T) are considered to represent two novel species of the genus Actinomadura, for which the names Actinomadura amylolytica sp. nov. (type strain YIM 77502(T) = DSM 45822(T) = CCTCC AA 2012024(T)) and Actinomadura cellulosilytica sp. nov. (type strain YIM 77510(T) = DSM 45823(T) = CCTCC AA 2012023(T)) are proposed. PMID:25944084

  16. Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp. nov., Thauera aminoaromatica sp. nov., and Azoarcus buckelii sp. nov

    Microsoft Academic Search

    Tahar Mechichi; Erko Stackebrandt; Nasser Gad'on; Georg Fuchs

    2002-01-01

    Six strains of denitrifying bacteria isolated from various oxic and anoxic habitats on different monocyclic aromatic substrates were characterized by sequencing 16S rRNA genes, determining physiological and morphological traits, and DNA-DNA hybridization. According to these criteria, strains S100, SP and LG356 were identified as members of Thauera aromatica. Strains B5-1 and B5-2 were tentatively affiliated to the species Azoarcus tolulyticus.

  17. Substrate specificities of the chloromuconate cycloisomerases from Pseudomonas sp. B13, Ralstonia eutropha JMP134 and Pseudomonas sp. P51

    Microsoft Academic Search

    M. D. Vollmer; U. Schell; V. Seibert; S. Lakner; M. Schlömann

    1999-01-01

    The chloromuconate cycloisomerase of Pseudomonas sp. B13 was purified from 3-chlorobenzoate-grown wild-type cells while the chloromuconate cycloisomerases of Ralstonia eutropha JMP134 (pJP4) and Pseudomonas sp. P51 (pP51) were purified from Escherichia coli strains expressing the corresponding gene. Kinetic studies were performed with various chloro-, fluoro-, and methylsubstituted\\u000a cis,cis-muconates. 2,4-Dichloro-cis,cis-muconate proved to be the best substrate for all three chloromuconate cycloisomerases.

  18. 40 CFR 180.1120 - Streptomyces sp. strain K61; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 2011-07-01 false Streptomyces sp. strain K61; exemption from the requirement...Tolerances § 180.1120 Streptomyces sp. strain K61; exemption from the requirement... The biological pesticide Streptomyces sp. strain K61 is exempted from the...

  19. 40 CFR 180.1075 - Colletotrichum gloeosporioides f. sp. aeschynomene; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...false Colletotrichum gloeosporioides f. sp. aeschynomene; exemption from the requirement...1075 Colletotrichum gloeosporioides f. sp. aeschynomene; exemption from the requirement...mycoherbicide Colletotrichum gloeosporioides f. sp. aeschynomene in or on the...

  20. 40 CFR 180.1120 - Streptomyces sp. strain K61; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 2010-07-01 false Streptomyces sp. strain K61; exemption from the requirement...Tolerances § 180.1120 Streptomyces sp. strain K61; exemption from the requirement... The biological pesticide Streptomyces sp. strain K61 is exempted from the...

  1. Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov.

    PubMed

    Behrendt, Undine; Ulrich, Andreas; Schumann, Peter

    2003-09-01

    Strains of fluorescent pseudomonads, isolated from the phyllosphere of grasses, were analysed by a polyphasic approach in order to clarify their interspecific position. Classification on the basis of ribotyping revealed six genotypes; four of these, which could be differentiated clearly from each other and from Pseudomonas species with validly published names on the basis of phenotypic features, were chosen for detailed phylogenetic analysis. DNA-DNA hybridization studies among representative strains of the four genotypes and closely related Pseudomonas species, determined by comparison of 16S rDNA sequences, showed that three of the studied ribotypes represented novel species. Two of them were related to mainly saprophytic fluorescent pseudomonads and could be easily distinguished by a negative arginine dihydrolase reaction. One ribotype, also characterized by a negative arginine dihydrolase reaction, was closely related to potentially plant-pathogenic fluorescent pseudomonads and differed in certain phenotypic features from its phylogenetic neighbours. As a consequence of the phenotypic and phylogenetic analyses, Pseudomonas trivialis sp. nov. (type strain: P 513/19(T)=DSM 14937(T)=LMG 21464(T)), Pseudomonas poae sp. nov. (type strain: P 527/13(T)=DSM 14936(T)=LMG 21465(T)) and Pseudomonas congelans sp. nov. (type strain: P 538/23(T)=DSM 14939(T)=LMG 21466(T)) are proposed. PMID:13130034

  2. Phytohabitans flavus sp. nov., Phytohabitans rumicis sp. nov. and Phytohabitans houttuyneae sp. nov., isolated from plant roots, and emended description of the genus Phytohabitans.

    PubMed

    Inahashi, Yuki; Matsumoto, Atsuko; Omura, Satoshi; Takahashi, Yoko

    2012-11-01

    An actinomycete strain, designated K09-0627(T), was isolated from the roots of an orchid collected in Okinawa Prefecture, Japan. Two actinomycete strains K11-0047(T) and K11-0057(T) were isolated from the roots of Rumex acetosa and Houttuynia cordata collected in Kanagawa Prefecture, Japan. 16S rRNA gene sequence analyses indicated that the isolates belonged to the genus Phytohabitans, and that they were closely related to each other and to Phytohabitans suffuscus K07-0523(T). The DNA-DNA relatedness values between the three isolates and Phytohabitans suffuscus were below 70%. On the basis of phylogenetic analysis, DNA-DNA relatedness values and phenotypic characteristics, the strains should be classified as novel species in the genus Phytohabitans, for which the names Phytohabitans flavus sp. nov. (type strain, K09-0627(T)=JCM 17387(T)=NBRC 107702(T)=DSM 45551(T)), Phytohabitans rumicis sp. nov. (type strain, K11-0047(T)=JCM 17829(T)=NBRC 108638(T)=BCC 48146(T)) and Phytohabitans houttuyneae sp. nov. (type strain, K11-0057(T)=JCM 17830(T)=NBRC 108639(T)=BCC 48147(T)) are proposed. PMID:22228668

  3. UNA PLANTA NUEVA DE LA SIERRA DE SEGURA (ESPAA), SUCCISELLA ANDREAE-MOUNAE, SP. NOV. (DIPSACACEAE)

    E-print Network

    Herrera, Carlos M.

    la Sierra de Segura (España), Succi- sella andreae-molinae, sp, nov. (Dipsacaceae). Anales Jard. Bot andreae-molinae, sp. nov. (Dipsacaceae). Anales Jard. Bot. Madrid 51(2): 249-254 (in Spa- nish). A new

  4. Biosorption characteristics of copper (II), chromium (III), nickel (II), and lead (II) from aqueous solutions by Chara sp. and Cladophora sp.

    PubMed

    Elmaci, Ay?e; Yonar, Taner; Ozengin, Nihan

    2007-09-01

    The aim of this research was to expose individual removals of copper, chromium, nickel, and lead from aqueous solutions via biosorption using nonliving algae species, Chara sp. and Cladophora sp. Optimum pH values for biosorption of copper (II), chromium (III), nickel (II), and lead (II) from aqueous solutions were determined to be 6, 7, 7, and 3 for Cladophora sp. and 5, 3, 5, and 4 for Chara sp. respectively. Maximum adsorption capacities of Chara sp. [10.54 for chromium (III) and 61.72 for lead (II)] and Cladophora sp. [6.59 for chromium (III) and 16.75 and 23.25 for lead (II)] for chromium (III) and lead (II) are similar. On the other hand, copper (II) and nickel (II) biosorption capacity of Cladophora sp. [14.28 for copper (II) and 16.75 for nickel (II)] is greater than Chara sp. [6.506 for copper (II) and 11.76 for nickel (II)]. Significantly high correlation coefficients indicated for the Langmuir adsorption isotherm models can be used to describe the equilibrium behavior of copper, chromium, nickel, and lead adsorption onto Cladophora sp. and Chara sp. PMID:17910369

  5. An Insight into the Diverse Roles of Surfactant Proteins, SP-A and SP-D in Innate and Adaptive Immunity

    PubMed Central

    Nayak, Annapurna; Dodagatta-Marri, Eswari; Tsolaki, Anthony George; Kishore, Uday

    2012-01-01

    Surfactant proteins SP-A and SP-D are hydrophilic, collagen-containing calcium-dependent lectins, which appear to have a range of innate immune functions at pulmonary as well as extrapulmonary sites. These proteins bind to target ligands on pathogens, allergens, and apoptotic cells, via C-terminal homotrimeric carbohydrate recognition domains, while the collagen region brings about the effector functions via its interaction with cell surface receptors. SP-A and SP-D deal with various pathogens, using a range of innate immune mechanisms such as agglutination/aggregation, enhancement of phagocytosis, and killing mechanisms by phagocytic cells and direct growth inhibition. SP-A and SP-D have also been shown to be involved in the control of pulmonary inflammation including allergy and asthma. Emerging evidence suggest that SP-A and SP-D are capable of linking innate immunity with adaptive immunity that includes modulation of dendritic cell function and helper T cell polarization. This review enumerates immunological properties of SP-A and SP-D inside and outside lungs and discusses their importance in human health and disease. PMID:22701116

  6. A thermostable cyclodextrin glycosyltransferase from Thermoanaerobacter sp. 5K

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cyclodextrin glycosyltransferase (CGTase) from the thermophilic anaerobe Thermoanaerobacter sp. 5K was purified and characterized. The enzyme was purified with ammonium sulfate precipitation followed by a-CD-bound, epoxy-activated Sepharose 6B affinity chromatography. Molecular weight of the purifie...

  7. BATTERIES, SP-II Page 1 of 11

    E-print Network

    Udgaonkar, Jayant B.

    BATTERIES, SP-II Page 1 of 11 NATIONAL INSTITUTE OF IMMUNOLOGY ArunaAsaf Ali Marg, New Delhi-110067 of Immunology (NII), Aruna Asaf Ali Road, New Delhi-110067 invites sealed tenders in two bid system (Technical into Vender Registration on `Annual Contract' basis for the supply of "Batteries". Tender Documents along

  8. Ring B aromatic norpimarane glucoside from a Xylaria sp.

    PubMed

    Isaka, Masahiko; Yangchum, Arunrat; Auncharoen, Patchanee; Srichomthong, Kitlada; Srikitikulchai, Prasert

    2011-02-25

    A novel 20-norpimarane glucoside, xylopimarane (1), together with the known sphaeropsidin C (2) and clonostachydiol (3), was isolated from the fungus Xylaria sp. BCC 4297. Compound 1 exhibited cytotoxicity to cancer cell lines KB, MCF-7, and NCI-H187 with respective IC(50) values of 1.0, 13, and 65 ?M. PMID:21226484

  9. Nonlocal Regularization and Renormalization of Sp(2) Symmetric BRST Theories

    Microsoft Academic Search

    V. Calian; G. Stoenescu

    2003-01-01

    An extension of the nonlocal regularization scheme is formulated for the Sp(2) symmetric Lagrangian BRST quantization. It provides a systematic treatment of the anomalous quantum master equations and allows to subtract the divergences as well as to calculate genuine higher loop BRST and anti-BRST anomalies.

  10. Gonderia herpestis n. sp. from the egyptian mongoose, Herpestis ichneumon

    Microsoft Academic Search

    M. H. Haiba

    1958-01-01

    Egyptian mongoose of different sexes and degrees of maturity have been investigated for Piroplasma infection. Out of a total of 13 captured individuals from the various above mentioned Egyptian localities, 8 were detected to be infected with a new species of Gonderia for which the name „Gonderia herpestis“ n. sp. was proposed. Among these, 6 were young, with 2 infected

  11. Eubacteriurn exiguum sp. nov., Isolated from Human Oral Lesions

    Microsoft Academic Search

    SERGIO E. POCO; FUTOSHI NAKAZAWA; TETSURO IKEDA; MICHIKO SATO; TAKUICHI SATO; ETSURO HOSHINO

    1996-01-01

    Eubacterium exipum sp. nov. is the name proposed for organisms formerly described as Eubacteriurn group S strains and similar bacteria isolated from various types of oral lesions. This new species was established on the basis of the results of DNA-DNA hybridization experiments and DNA base composition determinations (G+C contents, 60 to 64 mol%). The results of an API ZYM analysis,

  12. Draft Genome Sequence of Rhodococcus sp. Strain 311R

    PubMed Central

    Ehsani, Elham; Jauregui, Ruy; Geffers, Robert; Jareck, Michael; Boon, Nico; Pieper, Dietmar H.

    2015-01-01

    Here, we report the draft genome sequence of Rhodococcus sp. strain 311R, which was isolated from a site contaminated with alkanes and aromatic compounds. Strain 311R shares 90% of the genome of Rhodococcus erythropolis SK121, which is the closest related bacteria. PMID:25999565

  13. Heterocyst formation and nitrogenase synthesis in Anabaena sp

    Microsoft Academic Search

    Alasdair Neilson; Rosmarie Rippka; Riyo Kunisawa

    1971-01-01

    When filaments from a culture of Anabaena sp. growing photoautotrophically with nitrate as a nitrogen source are placed in a nitrate-free mineral medium and incubated anaerobically in the light, the formation of heterocysts and the synthesis of nitrogenase both begin after a lag of about 24 hours. During the lag period, about 70% of the phycocyanin is destroyed. Under an

  14. SUPPORTING INFORMATION Synthesis of Highly Crystalline sp2

    E-print Network

    Zettl, Alex

    1 SUPPORTING INFORMATION Synthesis of Highly Crystalline sp2 -Bonded Boron Nitride Aerogels Michael characterization (XRD and nitrogen adsorption isotherms) of the BN aerogels discussed in the text, as well as a schematic detailing the crucible in which the synthesis of the aerogels is performed. #12;2 Figure S1

  15. T(ether) is a sp collaborative m

    E-print Network

    Cortes, Corinna

    Dávid Lak 1 T {dlakatos ABSTRACT T(ether) is a sp collaborative m objects. The ha reality, providin T(ether is held by t ACM 978-1-4503-282 http://dx.doi.org/10.1 ether): S oceptio katos1 , Matth Tangible Med Camb manipulati ether) is a syste b) Gestural inte e. (c) The UI pr ­­­­­­­­­­­­­­­­­­­­­­­­­­­­­ stitute

  16. Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium

    PubMed Central

    Han-Jen, Robson Ee; Wai-Fong, Yin; Kok-Gan, Chan

    2013-01-01

    Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various chain lengths and degrees of saturation at the C-3 position. We have isolated a bacterium, RB-44, from a site which was formally a landfill dumping ground. Using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis, this isolate was identified as a Pandoraea sp.which was then screened for AHL production using biosensors which indicated its quorum sensing properties. To identify the AHL profile of Pandoraea sp. RB-44, we used high resolution tandem mass spectrometry confirming that this isolate produced N-octanoylhomoserine lactone (C8-HSL). To the best of our knowledge, this is the first report that showed quorum sensing activity exhibited by Pandoraea sp. Our data add Pandoraea sp. to the growing number of bacteria that possess QS systems. PMID:24145919

  17. Kocuria salsicia sp. nov., isolated from salt-fermented seafood

    E-print Network

    Bae, Jin-Woo

    Kocuria salsicia sp. nov., isolated from salt- fermented seafood Ji-Hyun Yun,1 Seong Woon Roh,1 salt-fermented seafood in Korea. It was a Gram- positive, non-motile, coccus-shaped bacterium sediment (Kim et al., 2004), saline desert soil (Li et al., 2006), fermented food (Park et al., 2010a, b

  18. Leucobacter celer sp. nov., isolated from Korean fermented seafood

    E-print Network

    Bae, Jin-Woo

    Leucobacter celer sp. nov., isolated from Korean fermented seafood Na-Ri Shin, Min-Soo Kim, Mi, designated NAL101T , was isolated from gajami-sikhae, a traditional Korean fermented seafood made of flatfish ). Sikhae, a kind of traditional Korean food, also known as jeotgal, consists of fermented fish without

  19. Complete Genome Sequence of Antarctic Bacterium Psychrobacter sp. Strain G

    PubMed Central

    Che, Shuai; Song, Lai; Song, Weizhi; Yang, Meng

    2013-01-01

    Here, we report the complete genome sequence of Psychrobacter sp. strain G, isolated from King George Island, Antarctica, which can produce lipolytic enzymes at low temperatures. The genomics information of this strain will facilitate the study of the physiology, cold adaptation properties, and evolution of this genus. PMID:24051316

  20. Civilian and military missions SP-100 preliminary user requirements

    SciTech Connect

    Not Available

    1987-06-29

    This document defines the top level requirements of potential users of a space based nuclear electric power supply. This provides the SP-100 Project and information required to design the modular (10-1000 KWe) space power systems to meet the needs of most potential users.

  1. Polydactyly in the central pacific gecko, Lepidodactylus sp. (Squamata: Gekkonidae)

    USGS Publications Warehouse

    Bauer, A.M.; Hathaway, S.A.; Fisher, R.N.

    2009-01-01

    We report the first known case of naturally occurring polydactyly in a gekkotan lizard. A single individual from Palmyra Atoll exhibited a triplication of digit III of the m hand. No obvious teratogenic sources are present on the atoll and the causal factors of polydactyly in Lepidodactylus sp. remain unknown.

  2. Characterization of phenanthrene degradation by strain polyporus sp. S133.

    PubMed

    Hadibarata, Tony; Tachibana, Sanro

    2010-01-01

    Polyporus sp. S133, a fungus collected from contaminated soil, was used to degrade phenanthrene, a polycyclic aromatic hydrocarbon, in a mineral salt broth liquid culture. A maximal degradation rate (92%) was obtained when Polyporus sp. S133 was cultured for 30 days with agitation at 120 r/min, as compared to 44% degradation in non-agitated cultures. Furthermore, the degradation was affected by the addition of surfactants. Tween 80 was the most suitable surfactant for the degradation of phenanthrene by Polyporus sp. S133. The degradation rate increased as the amount of Tween 80 added increased. The rate in agitated cultures was about 2 times that in non-agitated cultures. The mechanism of degradation was determined through the identification of metabolites; 9,10-phenanthrenequinone, 2,2'-diphenic acid, phthalic acid, and protocatechuic acid. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Polyporus sp. S133 were detected during the incubation. The highest level of activity was shown by 1,2-dioxygenase (187.4 U/L) after 20 days of culture. PMID:20397398

  3. Condensed State Sp aces for Timed Petri Nets

    E-print Network

    Mailund, Thomas

    Condensed State Sp aces for Timed Petri Nets Søren Christ ensen 1 , Lars Michael Krist ensen 1.kristensen@unisa.edu.au Abstract. We present a state space methodf or Petri nets having a time concept based on a global clock to condense the usually infinite state space of such timed Petri nets into a finite state space without

  4. Nitrogen-driven sp3 transformation in carbon nitride materials

    E-print Network

    Yang, Peidong

    DLC .4 Extensive experimental effort has been placed on prepar- ing carbon nitride materials using of nitrogen-doped DLC indicate that carbon relaxes to sp2 bonding at less than 5% nitrogen composition.13 We -bonded DLC.4 Pulsed la- ser deposition PLD was used to generate energetic carbon species and a plasma

  5. Draft Genome Sequence of Pseudomonas sp. nov. H2.

    PubMed

    Loftie-Eaton, Wesley; Suzuki, Haruo; Bashford, Kelsie; Heuer, Holger; Stragier, Pieter; De Vos, Paul; Settles, Matthew L; Top, Eva M

    2015-01-01

    We report the draft genome sequence of Pseudomonas sp. nov. H2, isolated from creek sediment in Moscow, ID, USA. The strain is most closely related to Pseudomonas putida. However, it has a slightly smaller genome that appears to have been impacted by horizontal gene transfer and poorly maintains IncP-1 plasmids. PMID:25838493

  6. Improved Adhesives Containing CNT\\/SP1 Nano Fillers

    Microsoft Academic Search

    A. Wolf; A. Buchman; A. Eitan; T. Fine; Y. Nevo; A. Heyman; O. Shoseyov

    2012-01-01

    Carbon nanotubes (CNT) have stimulated research due to their wide range of applications. However, their existence as aggregates and the difficulty in debundling and dispersion limits the improvement of properties when used as fillers. Many techniques have been employed to obtain such dispersions including mechanical, ultrasonic, and solution mixing, resulting in limited effect. Attaching a protein moiety such as SP1

  7. Thermoactinomyces sacchari sp.nov., a Thermophilic Actinomycete Causing Bagassosis

    Microsoft Academic Search

    J. Lacey

    1971-01-01

    SUMMARY A new species of thermophilic monosporic actinomycete, isolated from mouldy sugar cane bagasse, is described as Thermoactinomyces sacchari sp.nov. It is distinguished from T. vulgaris Tsiklinsky by short tufted aerial mycelia that rapidly autolyse and are not always seen, by bearing spores on short sporophores, by its appearance on different culture media and by serological differences. Both species have

  8. Soil treatments against Fusarium oxysporum f. sp. vasinfectum race 4

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Few economically feasible disease management options are available for California cotton producers with fields infested with race 4 of Fusarium oxysporum f. sp. vasinfectum. For treating soil to reduce inoculum levels, past studies indicate that solarization and fumigation with metam-sodium may be a...

  9. Fusarium oxysporum f. sp. vasinfectum race 4 in California

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A brief review of research on Fusarium oxysporum Schlechtend.:Fr. f. sp. vasinfectum (Atk.) W.C. Snyder & H.N. Hans. race 4 in California is presented. Fusarium wilt has recently emerged as the dominant disease concern for cotton (Gossypium hirsutum L., G. barbadense L.) growers in California. An es...

  10. WHAT'S GRAPHENE? Mono or few layers of sp2 bonded

    E-print Network

    Mellor-Crummey, John

    WHAT'S GRAPHENE? · Mono or few layers of sp2 bonded carbon atoms in a honeycomb lattice 105cm2/Vs at RT. 1 Due to its unique transport properties, graphene is suitable for implementation sampling (EOS) timeresolved spectroscopy to optically pump and THz probe exfoliated graphene ribbons (GR

  11. Users guide to the Argonne SP scheduling system

    Microsoft Academic Search

    David A. Lifka; Mark W. Henderson; Karen Rayl

    1995-01-01

    During the past five years scientists discovered that modern UNIX workstations connected with ethernet and fiber networks could provide enough computational performance to compete with the supercomputers of the day. As this concept became increasingly popular, the need for distributed queuing and scheduling systems became apparent. Today, supercomputers, such as Argonne National Laboratory`s IBM SP system, can provide more CPU

  12. Biscalitheca suzanneana, N. Sp., from the uppermost Pennsylvanian of Texas

    USGS Publications Warehouse

    Mamay, S.H.

    1972-01-01

    Biscalitheca suzanneana, n. sp., is described from specimens found in an uppermost Pennsylvanian deposit in north-central Texas. The genus was identified primarily on the basis of impressions of the unique sporangial annuli. The occurrence establishes for Biscalitheca a stratigraphic range equivalent to nearly the entire Upper Pennsylvanian Series. ?? 1972.

  13. Cotoneaster sp. poisoning in a llama (Lama glama)

    Microsoft Academic Search

    Aurelie Gruss; Nathalie Priymenko

    A case of poisoning with a Cotoneaster sp. is described in a llama (Lama glama). The clinical signs were dyspnea, with congested mucous membranes, chewing, recumbency, and opisthotonos. Several hours after ingestion of the plant, the llama died, despite treatment with sodium thiosulfate and nitrite. Necropsy revealed approximately 676 g of leaves and fruit of Cotoneaster in the first compartment

  14. Characteristics of Monascus sp. isolated from Monascus fermentation products

    Microsoft Academic Search

    Ji Yeun Kim; Hae-Jin Kim; Jee-Hwan Oh; Inhyung Lee

    2010-01-01

    To screen for the strains suitable for industrial applications, 15 Monacus sp. were isolated from various Monascus fermentation products. The isolated strains were belonging to Monascus pilosus, Monascus purpureus, Monascus albidulus, and Monascus femeus based on the ITS sequence and morphological characteristics. They produced globular and hyaline conidia, and red, orange,\\u000a or hyaline ascomata except for M. fumeus, in which

  15. ORIGINAL PAPER Candida gelsemii sp. nov., a yeast

    E-print Network

    Thomson, James D.

    ORIGINAL PAPER Candida gelsemii sp. nov., a yeast of the Metschnikowiaceae clade isolated from+Business Media B.V. 2006 Abstract A new yeast species, Candida gelsemii, is described to accommodate three Metschnikowiaceae Á Gelsemium sempervirens Á Nectar alkaloids Á Gelsemine Á New yeast species Introduction Floral

  16. Lactobacillus kimchiensis sp. nov., isolated from a fermented food

    E-print Network

    Bae, Jin-Woo

    Lactobacillus kimchiensis sp. nov., isolated from a fermented food Jandi Kim,3 Joon Yong Kim,3 Min bacterium was isolated from a traditional fermented food, kimchi. The morphology, physiology, biochemical ). Kimchi is a traditional Korean fermented food made of various vegetables and spices (red pepper, garlic

  17. Leucobacter salsicius sp. nov., from a salt-fermented food

    E-print Network

    Bae, Jin-Woo

    Leucobacter salsicius sp. nov., from a salt- fermented food Ji-Hyun Yun,1 Seong Woon Roh,1,2 Min, Daejeon 305-806, Republic of Korea Strain M1-8T was isolated from jeotgal, a Korean salt-fermented food salt-fermented seafood. Shrimp jeotgal is made by mixing fresh tiny shrimps with rock salt, followed

  18. Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter

    E-print Network

    Bae, Jin-Woo

    Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter Na-Ri Shin, Mi , was isolated from a Korean alcohol fermentation starter. Optimal growth occurred at 37 6C, at pH 8 and in 1 was isolated from nuruk, which is an alcohol fermentation starter used as an amylolytic substance

  19. Eukiefferiella halvorseni sp. n. from Kashmir (India) (Diptera, Nematocera, Chironomidae)

    Microsoft Academic Search

    N. Caspers

    1990-01-01

    The male imago of Eukiefferiella halvorseni sp. n. is described from two specimens collected in Kashmir (India). The new species is a member of the E. gracei group. With respect to the hypopygial features it closely resembles E. fittkaui Lehmann, differing primarily by its extremely low AR. Another specimen probably belonging to the same species has been caught in an

  20. Draft Genome Sequence of Rhodococcus sp. Strain 311R.

    PubMed

    Ehsani, Elham; Jauregui, Ruy; Geffers, Robert; Jareck, Michael; Boon, Nico; Pieper, Dietmar H; Vilchez-Vargas, Ramiro

    2015-01-01

    Here, we report the draft genome sequence of Rhodococcus sp. strain 311R, which was isolated from a site contaminated with alkanes and aromatic compounds. Strain 311R shares 90% of the genome of Rhodococcus erythropolis SK121, which is the closest related bacteria. PMID:25999565

  1. FOOD FISH FACTS Sp4nlSh Mackerel

    E-print Network

    46 FOOD FISH FACTS Sp4nlSh Mackerel (Scomberomorus m~cul.1lw) Spanish mackerel are members of a large family of fish that include the tunas and other mackerels. All of these fish, although vary- ing of this family live in the open sea and are more or less migratory. Spanish mackerel are known

  2. CHEN 3650 SP14 -Lab 2 Two Tanks in Series

    E-print Network

    Ashurst, W. Robert

    volume balance, estimate how much liquid has been diverted to the overflow tank at the instant the slower that any excess liquid (should there be overfilling) is diverted into another (overflow) tankCHEN 3650 SP14 - Lab 2 Two Tanks in Series Part A ­ Computer Exercise This laboratory exercise

  3. Lutein production by Muriellopsis sp. in an outdoor tubular photobioreactor

    Microsoft Academic Search

    José A Del Campo; Herminia Rodr??guez; José Moreno; M. Ángeles Vargas; Joaqu??n Rivas; Miguel G Guerrero

    2001-01-01

    The effect of dilution rate, mixing and daily solar cycles on lutein and biomass productivity of the green unicellular alga Muriellopsis sp. has been studied, throughout the year, in an outdoor tubular photobioreactor. Highest productivity values, for both lutein (about 180 mg m?2 per day) and biomass (about 40 g (dry weight) m?2 per day) were achieved on May and

  4. Irrigation of pepper plant ( Capsicum sp.) with water containing acrolein

    Microsoft Academic Search

    Hugo A. Caldironi; Diego J. Bentivegna; Miles R. Rhea

    2004-01-01

    MAGNACIDE® H herbicide (a.i. acrolein (2-propenal)) is an aquatic herbicide applied through underwater injection into agricultural irrigation canals for the control of submerged aquatic weeds. In support of the products national registration in Argentina, additional information was required pertaining to the potential persistence of acrolein residuals in plants irrigated with water treated with this herbicide. Pepper plants (Capsicum sp.) were

  5. DEATH AND AUTHENTICITY PHI 500/REL 600, SP. 2010

    E-print Network

    Kovalev, Leonid

    DEATH AND AUTHENTICITY PHI 500/REL 600, SP. 2010 PROF. ED MOONEY, RELIGION AND PHILOSOPHY HL 501, M to ourselves, doing what we must) and death (its nothingness, its threats). For evoking and unraveling these enigmas and puzzles we can't do better than start with Tolstoy's short story, "The Death of Ivan Ilych

  6. Complete Genome Assembly of Corynebacterium sp. Strain ATCC 6931

    PubMed Central

    Daligault, H. E.; Davenport, K. W.; Minogue, T. D.; Bishop-Lilly, K. A.; Bruce, D. C.; Chain, P. S.; Coyne, S. R.; Frey, K. G.; Jaissle, J.; Koroleva, G. I.; Ladner, J. T.; Li, P-E.; Meincke, L.; Munk, A. C.; Palacios, G. F.; Redden, C. L.

    2014-01-01

    The genus Corynebacterium is best known for the pathogen C. diphtheriae; however, it contains mostly commensal and nonpathogenic, as well as several opportunistic, pathogens. Here, we present the 2.47-Mb scaffolded assembly of the type strain, Corynebacterium sp. ATCC 6931 (NCTC 1914), as deposited into GenBank under accession number CP008913. PMID:25342684

  7. Uranium accumulation by immobilized cells of a Citrobacter sp

    Microsoft Academic Search

    Lynne E. Macaskie; A. C. R. Dean

    1985-01-01

    Uranium was removed from challenge flows presented to immobilized cells of aCitrobacter sp. In excess of 90% of the presented metal was recovered, giving high yields of accumulated metal which could be subsequently released from the immobilized cellsin situ.

  8. Distributions centrales sur le produit semi-direct Sp(1)? H

    Microsoft Academic Search

    Raja Essoussi Mrad

    2001-01-01

    Let H be the field of quaternions, and Sp(1) the multiplicative group of quaternions of modulus 1. The group Sp(1) acts on H by left multiplication and we define the semi-direct product G=Sp(1)?H. We prove that the central distributions on G are of the form: S=C?1+?1?R, where C is a central distribution on Sp(1), R is a radial distribution on H,

  9. Resistance to Puccinia graminis f. sp. avenae in Barley Is Associated with the Rpg5 Locus.

    PubMed

    Dracatos, Peter; Singh, Davinder; Fetch, Tom; Park, Robert

    2015-04-01

    In barley, gene Rpg5 was first identified for providing resistance to the rye stem rust pathogen (Puccinia graminis f. sp. secalis). A subsequent study determined that Rpg5 is required for rpg4-mediated resistance to the wheat stem rust pathogen (P. graminis f. sp. tritici) including pathotype TTKSK ("Ug99"), which poses a major threat to global wheat and barley production. Based on the effectiveness of Rpg5 against P. graminis f. sp. tritici and P. graminis f. sp. secalis, we assessed whether it also conferred resistance to the oat stem rust pathogen (P. graminis f. sp. avenae). A barley F8 recombinant inbred line (RIL) population was produced by crossing 'Q21861' (Rpg1 and Rpg5) with '73-G1' (Rpg1), which is susceptible to P. graminis f. sp. avenae, P. graminis f. sp. secalis, and some pathotypes of P. graminis f. sp. tritici. Seedling tests were performed on the F8 RIL population using Australian pathotypes of P. graminis f. sp. tritici, P. graminis f. sp. secalis, P. graminis f. sp. avenae, and a putative somatic hybrid between P. graminis f. sp. tritici and P. graminis f. sp. secalis known as the 'Scabrum' rust. Segregation in the responses to all rust isolates for the RILs was identical (50 resistant: 52 susceptible), and fitted a 1:1 ratio (X(2) = 0.039, P = 0.843), indicating that resistance to all isolates was monogenetically inherited. Screening of the RILs and the parental lines with perfect markers for the functional Rpg1 and Rpg5 resistance alleles indicated that Rpg1 was fixed, while Rpg5 was positive in all resistant lines and negative in all susceptible lines. This suggests that different formae speciales of P. graminis may share common effectors, and that the Rpg5 locus confers resistance to both P. graminis f. sp. tritici and P. graminis f. sp. secalis and the heterologous formae speciales of P. graminis, P. graminis f. sp. avenae. PMID:25870923

  10. The sea urchin complement homologue, SpC3, functions as an opsonin

    Microsoft Academic Search

    Lori A. Clow; David A. Raftos; Paul S. Gross; L. Courtney Smith

    2004-01-01

    The purple sea urchin Strongylocentrotus purpuratus expresses a homologue of complement component C3 (SpC3), which acts as a humoral opsonin. Significantly increased phagocytic activity was evident when yeast target cells were opsonized after incubation with coelomic fluid containing SpC3. SpC3 could be detected on the surface of yeast, and phagocytic activity could be inhibited by an anti-SpC3 antibody. This indicates

  11. Degradation of Phycobilisomes in Synechocystis sp. PCC6803

    PubMed Central

    Baier, Antje; Winkler, Wiebke; Korte, Thomas; Lockau, Wolfgang; Karradt, Anne

    2014-01-01

    When cyanobacteria acclimate to nitrogen deficiency, they degrade their large (3–5-MDa), light-harvesting complexes, the phycobilisomes. This massive, yet specific, intracellular degradation of the pigmented phycobiliproteins causes a color change of cyanobacterial cultures from blue-green to yellow-green, a process referred to as chlorosis or bleaching. Phycobilisome degradation is induced by expression of the nblA gene, which encodes a protein of ?7 kDa. NblA most likely acts as an adaptor protein that guides a Clp protease to the phycobiliproteins, thereby initiating the degradation process. Most cyanobacteria and red algae possess just one nblA-homologous gene. As an exception, the widely used “model organism” Synechocystis sp. PCC6803 expresses two such genes, nblA16803 and nblA26803, both of whose products are required for phycobilisome degradation. Here, we demonstrate that the two NblA proteins heterodimerize in vitro and in vivo using pull-down assays and a Förster energy-transfer approach, respectively. We further show that the NblA proteins form a ternary complex with ClpC (the HSP100 chaperone partner of Clp proteases) and phycobiliproteins in vitro. This complex is susceptible to ATP-dependent degradation by a Clp protease, a finding that supports a proposed mechanism of the degradation process. Expression of the single nblA gene encoded by the genome of the N2-fixing, filamentous cyanobacterium Nostoc sp. PCC7120 in the nblA1/nblA2 mutant of Synechocystis sp. PCC6803 induced phycobilisome degradation, suggesting that the function of the NblA heterodimer of Synechocystis sp. PCC6803 is combined in the homodimeric protein of Nostoc sp. PCC7120. PMID:24610785

  12. An air itinerary choice model based on a mixed RP/SP dataset

    E-print Network

    Bierlaire, Michel

    An air itinerary choice model based on a mixed RP/SP dataset Bilge Atasoy Michel Bierlaire April/SP dataset. The aim of the combination of the two datasets is to exploit the variability of the SP data is modeled as a latent class. In this study we develop an itinerary choice model based on a real dataset

  13. 76 FR 72505 - Proposed Collection; Comment Request for Form 2290/SP/FR

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-23

    ...Collection; Comment Request for Form 2290/SP/FR AGENCY: Internal Revenue Service...soliciting comments concerning Form 2290/SP/FR Heavy Highway Vehicle Use Tax Return...Number: 1545-0143. Abstract: Form 2290/SP/FR is used to compute and report the...

  14. Saturation Mutagenesis of 2,4-DNT Dioxygenase of Burkholderia sp. Strain DNT

    E-print Network

    Wood, Thomas K.

    Saturation Mutagenesis of 2,4-DNT Dioxygenase of Burkholderia sp. Strain DNT for Enhanced this study indicated that the 2,4-DNT dioxygenases of Burkholderia sp. strain DNT and B. cepacia R34 are more). Burkholderia sp. strain DNT was isolated from water samples from Waconda Bay near the Volunteer Army Ammunition

  15. Transcriptional regulation of cell-specific expression of the human cystathionine beta -synthase gene by differential binding of Sp1/Sp3 to the -1b promoter.

    PubMed

    Ge, Y; Matherly, L H; Taub, J W

    2001-11-23

    Cystathionine beta-synthase (CBS) catalyzes the condensation of serine and homocysteine to form cystathionine, an intermediate step in the synthesis of cysteine. We previously characterized the CBS -1b minimal promoter (-3792 to -3667) and found that Sp1/Sp3, nuclear factor Y, and USF-1 were involved in the regulation of basal promoter activity (Ge, Y., Konrad, M. A., Matherly, L. H., Taub, J. W. (2001) Biochem. J. 357, 97-105). In this study, the critical cis-elements and transcription factors in the CBS -1b upstream region (-4046 to -3792) were examined in HT1080 and HepG2 cells, which differ approximately 10-fold in levels of CBS transcripts transcribed from the CBS -1b promoter. In DNase I footprint and gel shift analyses and transient transfections of mutant CBS -1b promoter constructs into HT1080 and HepG2 cells, transcriptionally important roles for Sp1/Sp3 binding to three GC boxes and one GT box and for binding of myeloid zinc finger 1-like proteins to two myeloid zinc finger 1 elements were indicated. In gel shift assays, very low levels of Sp1/Sp3 DNA-protein complexes were detected in HT1080 cells compared with HepG2 cells despite comparable levels of nuclear factor Y and USF-1 binding and similar levels of Sp1 and Sp3 proteins on Western blots. Mixing of HT1080 and HepG2 nuclear extracts resulted in no difference in total Sp factor binding in gel shift assays, thus excluding a role for an unknown activator or inhibitor in the disparate Sp1/Sp3 binding between the lines. Increased Sp1/Sp3 binding in gel shift assays was observed upon treatment of HT1080 nuclear extracts with protein kinase A, and decreased Sp1/Sp3 binding resulted from treatment of HepG2 nuclear extracts with calf alkaline phosphatase, suggesting a role for changes in Sp1/Sp3 phosphorylation in transcription factor binding and transactivation of the CBS -1b promoter. Characterization of CBS promoter structure and function should clarify the molecular bases for variations in CBS gene expression in genetic diseases and the relationship between CBS and Down syndrome. PMID:11562358

  16. INTERACTION BETWEEN AN AMMONIUM-OXIDIZER, 'NITROSOMONAS' SP., AND TWO HETEROTROPHIC BACTERIA, 'NOCARDIA ATLANTICA' AND 'PSEUDOMONAS' SP.: A NOTE

    EPA Science Inventory

    Closed culture experiments showed that the ability of an estuarine chemolithotrophic ammonium-oxidizer, Nitrosomonas sp., to convert ammonium to nitrite was substantially increased by as much as 150% and 50% when grown in the presence of two heterotrophs isolated from the same en...

  17. Identification of fluorinases from Streptomyces sp MA37, Norcardia brasiliensis, and Actinoplanes sp N902-109 by genome mining.

    PubMed

    Deng, Hai; Ma, Long; Bandaranayaka, Nouchali; Qin, Zhiwei; Mann, Greg; Kyeremeh, Kwaku; Yu, Yi; Shepherd, Thomas; Naismith, James H; O'Hagan, David

    2014-02-10

    The fluorinase is an enzyme that catalyses the combination of S-adenosyl-L-methionine (SAM) and a fluoride ion to generate 5'-fluorodeoxy adenosine (FDA) and L-methionine through a nucleophilic substitution reaction with a fluoride ion as the nucleophile. It is the only native fluorination enzyme that has been characterised. The fluorinase was isolated in 2002 from Streptomyces cattleya, and, to date, this has been the only source of the fluorinase enzyme. Herein, we report three new fluorinase isolates that have been identified by genome mining. The novel fluorinases from Streptomyces sp. MA37, Nocardia brasiliensis, and an Actinoplanes sp. have high homology (80-87 % identity) to the original S. cattleya enzyme. They all possess a characteristic 21-residue loop. The three newly identified genes were overexpressed in E. coli and shown to be fluorination enzymes. An X-ray crystallographic study of the Streptomyces sp. MA37 enzyme demonstrated that it is almost identical in structure to the original fluorinase. Culturing of the Streptomyces sp. MA37 strain demonstrated that it not only also elaborates the fluorometabolites, fluoroacetate and 4-fluorothreonine, similar to S. cattleya, but this strain also produces a range of unidentified fluorometabolites. These are the first new fluorinases to be reported since the first isolate, over a decade ago, and their identification extends the range of fluorination genes available for fluorination biotechnology. PMID:24449539

  18. Digenetic trematodes, Acanthatrium sp. and Lecithodendrium sp., as vectors of Neorickettsia risticii, the agent of Potomac horse fever.

    PubMed

    Pusterla, N; Johnson, E M; Chae, J S; Madigan, J E

    2003-12-01

    Neorickettsia (formerly Ehrlichia) risticii, the agent of Potomac horse fever (PHF), has been recently detected in trematode stages found in the secretions of freshwater snails and in aquatic insects. Insectivores, such as bats and birds, may serve as the definitive host of the trematode vector. To determine the definitive helminth vector, five bats (Myotis yumanensis) and three swallows (Hirundo rustica, Tachycineta bicolor) were collected from a PHF endemic location in northern California. Bats and swallows were dissected and their major organs examined for trematodes and for N. risticii DNA using a nested polymerase chain reaction (PCR) assay. Adult digenetic trematodes, Acanthatrium sp. and/or Lecithodendrium sp., were recovered from the gastrointestinal tract of all bats and from one swallow. The intestine of three bats, the spleen of two bats and one swallow as well as the liver of one swallow tested PCR positive for N. risticii. From a total of seven pools of identical digenetic trematodes collected from single hosts, two pools of Acanthatrium sp. and one pool of Lecithodendrium sp. tested PCR positive. The results of this investigation provide preliminary evidence that at least two trematodes in the family Lecithodendriidae are vectors of N. risticii. The data also suggest that bats and swallows not only act as a host for trematodes but also as a possible natural reservoir for N. risticii. PMID:14627451

  19. Whole-Genome Sequence of Enterobacter sp. Strain SST3, an Endophyte Isolated from Jamaican Sugarcane (Saccharum sp.) Stalk Tissue

    PubMed Central

    Gan, Han Ming; McGroty, Sean E.; Chew, Teong Han; Chan, Kok Gan; Buckley, Larry J.; Savka, Michael A.

    2012-01-01

    Enterobacter sp. strain SST3 is an endophytic bacterium isolated from Saccharum spp. Here we present its annotated draft genome that may shed light on its role as a bacterial endophyte of sugarcane. To our knowledge, this is the first genome announcement of a sugarcane-associated bacterium from the genus Enterobacter. PMID:23045495

  20. Neotobrilus nicsmolae n. sp. (Tobrilidae: Nematoda) and Chronogaster carolinensis n. sp. (Chronogasteridae: Nematoda) from Lake Phelps, North Carolina

    PubMed Central

    Abebe, Eyualem; Ferebee, Briana; Taylor, Tarreyca; Mundo-Ocampo, Manuel; Mekete, Tesfamariam; De Ley, Paul

    2013-01-01

    Two new species, Neotobrilus nicsmolae n. sp. and Chronogaster carolinensis n. sp. are described from a small, acidic, temperate, natural lake in North Carolina. N. nicsmolae n. sp. comes close to three members of the genus reported from North America, N. filipjevi, N. longus, and N. hopei. However, N. nicsmolae is unique with in the genus in having a combination of characters: size smaller than 1,700 ?m, shorter outer labial and cephalic setae, tail shorter than 250 ?m, last ventromedian supplement close (about 5 ?m) to cloacal opening, spicule length of 61 to 85 ?m, flagelloid sperm, and possession of subterminal setae. Assessment of relationships among clades within the Triplonchida using DNA sequences of the D2D3 expansion segment of the LSU rDNA showed that the family Trichodoridae and the genus Tripyla were recovered as monophyletic. The genus Tobrilus was recovered as monophyletic in the neighbor-joining and maximum likelihood trees, but that was not so in the maximum-parsimony tree. The separation among genera of the Trichodoridae, i.e., Trichodorus and Paratrichodorus, was not clear-cut in all phylograms. Chronogaster carolinensis n. sp. in having one ventral mucro with no spine and vacuolated lateral glandular bodies comes close to C. typica and C. ethiopica but differs from all hitherto known species in a combination of characteristics: in having long cephalic setae, long stoma, crystalloid bodies, vacuolated lateral glandular bodies, and a tail terminus with blunt ventral mucro, and its lack of lateral line. PMID:23589662

  1. Role of Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 as Bioindicators and Immobilizers of Chromium in a Contaminated Natural Environment

    PubMed Central

    Millach, Laia; Solé, Antoni; Esteve, Isabel

    2015-01-01

    The aim of this work was to study the potential of the two phototrophic microorganisms, both isolated from Ebro Delta microbial mats, to be used as bioindicators and immobilizers of chromium. The results obtained indicated that (i) the Minimum Metal Concentration (MMC) significantly affecting Chlorophyll a intensity in Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 was 0.25?µM and 0.75?µM, respectively, these values being lower than those established by current legislation, and (ii) Scenedesmus sp. DE2009 was able to immobilize chromium externally in extracellular polymeric substances (EPS) and intracellularly in polyphosphate (PP) inclusions. Additionally, this microorganism maintained high viability, including at 500?µM. Based on these results, we postulate that Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 are good chromium-indicators of cytotoxicity and, further, that Scenedesmus sp. DE2009 plays an important role in immobilizing this metal in a contaminated natural environment.

  2. Mixed phase, sp2-sp3 bonded, and disordered few layer graphene-like nanocarbon: Synthesis and characterizations

    NASA Astrophysics Data System (ADS)

    Kumar, Arvind; Patil, Sumati; Joshi, Anupama; Bhoraskar, Vasant; Datar, Suwarna; Alegaonkar, Prashant

    2013-04-01

    We report on a method for the synthesis of Graphene-like nanocarbon (GNC) sheets. The obtained GNC contain mixed phase, sp2-sp3 bonded, and few atom layer of disordered carbon network. Initially, the mixture of soft wood charcoal (C), potassium nitrate (KNO3), and sulfur (S) was subjected to the combustion process. The obtained (as-synthesized) samples were intercalated (in H2SO4 (98%): HNO3 (60%), 48 h at 300 K) and subsequently annealed at 1000 °C for ˜60 s. The as-synthesized, intercalated, and annealed samples were studied using number of characterization techniques. The Raman spectra (at ? = 514 nm) recorded for the as-synthesized samples showed five prominent peaks, namely, for amorphous-Carbon (a-C), D doublet, small G, and 2D band. The position and intensity of the recorded peaks were varied for the intercalated and annealed samples. Our SEM analysis revealed that, the area of the GNC sheets varied typically in the range of 10 to 20 ?m2. The analysis of HRTEM/SAED together showed that the intercalated samples contained a-C phase, whereas, short range ordering was observed for the annealed samples. The tunneling spectra recorded for the annealed GNC sheets showed V-shaped local density of states with the peak present near the minima. Our analysis revealed that, the sp2 chains and the polycyclic carbon rings (PCR) could be formed in the host ta-C matrix generating mixed sp2-sp3 bonded carbon network along with the local disorder. Details are presented.

  3. Membrane fatty acids adaptive profile in the simultaneous presence of arsenic and toluene in Bacillus sp. ORAs2 and Pseudomonas sp. ORAs5 strains

    Microsoft Academic Search

    Milva Pepi; Hermann J. Heipieper; Janett Fischer; Marcella Ruta; Margherita Volterrani; Silvano E. Focardi

    2008-01-01

    Bacillus sp. ORAs2 and Pseudomonas sp. ORAs5, two arsenic-resistant bacterial strains previously isolated from sediments of the Orbetello Lagoon, Italy, were\\u000a tested for their adaptation to mixed contaminants on the level of membrane fatty acid composition. The two bacterial strains\\u000a were characterized by high levels of arsenic resistance, and Pseudomonas sp. ORAs5 was also shown to be solvent-tolerant. The bacterial

  4. Draft Whole-Genome Sequence of Serratia sp. Strain TEL, Associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae) Isolated from a Grassland in South Africa

    PubMed Central

    Featherston, Jonathan; Gray, Vincent M.

    2015-01-01

    Here, we report on the draft genome sequence of Serratia sp. strain TEL, associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae, KM492926) isolated from a grassland in Suikerbosrand Nature Reserve near Johannesburg in South Africa. Serratia sp. strain TEL has a genome size of 5,000,541 bp with 4,647 genes and a G+C content of 59.1%. PMID:26159531

  5. Cisplatin in 5% Ethanol Eradicates Cisplatin-Resistant Lung Tumor by Killing Lung Cancer Side Population (SP) Cells and Non-SP Cells

    PubMed Central

    Niu, Qi; Wang, Wei; Li, Yong; Ruden, Douglas M.; Li, Qian; Wang, Fenghua

    2013-01-01

    Cancer side population (SP) cells with cancer stem cell-like properties are thought to be responsible for lung cancer chemotherapy resistance and currently no drug can efficiently target them. Breast cancer resistance protein (BRCP/ABCG2) is a major drug transporter in protecting lung cancer SP cells from cytotoxic agents. We showed that a low concentration of ethanol, which inhibits many membrane proteins, inhibits ABCG2 in lung cancer SP cells. Furthermore, cytotoxic cisplatin (DDP) in 5% (vol/vol) ethanol kills SP plus non-SP cancer cells better than either treatment alone in eradicating chemoresistant lung tumors. We found that 5% ethanol did not reduce ABCG2 protein levels, but significantly reduced ABCG2 protein function by a Hoechst 33342 extrusion assay, an ATPase activity assay, and transmission electron microscopy. Further, DDP in 5% ethanol (5% ethanol–DDP) induced apoptosis of the SP plus non-SP cancer cells both in vitro and in vivo. In DDP-resistant A549/DDP lung tumor-bearing Balb/C nude mice, intratumoral injection of 5% ethanol–DDP regressed tumors and significantly improved survivals compared with 5% ethanol, DDP alone, or control. Intratumoral injection of 5% ethanol–DDP helped eradicate tumors in 30% (3/10) of the mice after 4 weeks treatment. By killing SP and non-SP cancer cells, 5% ethanol–DDP could eradicate DDP-resistant lung tumor and extend survival, providing a novel way to improve chemoresistant lung cancer survival for clinic. PMID:24009622

  6. Additive Effect of Sp1 and Sp3 in Regulation of the ED-L1E Promoter of the EBV LMP 1 Gene in Human Epithelial Cells

    Microsoft Academic Search

    Chi-Neu Tsai; Chiu-Mei Lee; Chih-Kai Chien; Szu-Cheng Kuo; Yu-Sun Chang

    1999-01-01

    The ED-L1E promoter of the LMP 1 gene is a GC box-containing promoter. To test if Sp1\\/Sp3 are important for modulating ED-L1E promoter activity through the GC box, site-specific mutation and deletion constructs carrying a reporter gene were transfected into NPCTW076 and C33A cells. Results showed that deletion or mutation of the GC box abolished ED-L1E activity. Association of Sp1\\/Sp3

  7. The effect of aspect ratio and sp2/sp3 content on the field emission properties of carbon films grown by Ns-spiked PECVD

    SciTech Connect

    Tong, W.; Felter, T.E. [Lawrence Livermore National Lab., CA (United States); Pan, L.S. [Candescent Technologies Corp., San Jose, CA (United States); Anders, S.; Cossy-Facre, A.; Stammler, T. [Lawrence Berkeley National Lab., CA (United States). Advanced Light Source Div.

    1998-04-01

    The authors have deposited carbon films from mixtures of methane and N{sub 2} using Plasma Enhanced Chemical Vapor Deposition. By changing the percentage of N{sub 2} in the feed gas, they were able to produce films that have various aspect ratios and sp{sup 2}/sp{sup 3} contents. The film with the highest field emission contains spears of aspect ratio of 10:1. They also found that in their sp{sup 3}-rich films, higher sp{sup 2} content enhanced field emission. This is ascribed to improved charge transport to the field emission sites.

  8. Direct Aerobic Carbonylation of C(sp(2))-H and C(sp(3))-H Bonds through Ni/Cu Synergistic Catalysis with DMF as the Carbonyl Source.

    PubMed

    Wu, Xuesong; Zhao, Yan; Ge, Haibo

    2015-04-22

    The direct carbonylation of aromatic sp(2) and unactivated sp(3) C-H bonds of amides was achieved via nickel/copper catalysis under atmospheric O2 with the assistance of a bidentate directing group. The sp(2) C-H functionalization showed high regioselectivity and good functional group compatibility. The sp(3) C-H functionalization showed high site-selectivity by favoring the C-H bonds of ?-methyl groups over those of the ?-methylene, ?- or ?-methyl groups. Moreover, this reaction showed a predominant preference for functionalizing the ?-methyl over ?-phenyl group. Mechanistic studies revealed that nickel/copper synergistic catalysis is involved in this process. PMID:25815529

  9. Purification of surfactant protein D (SP-D) from pooled amniotic fluid and bronchoalveolar lavage.

    PubMed

    Dodagatta-Marri, Eswari; Qaseem, Asif S; Karbani, Najmunisa; Tsolaki, Anthony G; Waters, Patrick; Madan, Taruna; Kishore, Uday

    2014-01-01

    Surfactant protein SP-D is a multimeric collagenous lectin, called collectin. SP-D is a multifunctional, pattern recognition innate immune molecule, which binds in a calcium dependent manner to an array of carbohydrates and lipids, thus offering resistance to invading pathogens, allergen challenge, and pulmonary inflammation. SP-D is predominantly found in the endoplasmic reticulum of type 2 pneumocytes and in the secretory granules of Clara or non-ciliated bronchiolar cells. The highest expression of SP-D is observed in the distal airways and alveoli. There is also an extra pulmonary existence of SP-D. The common sources of native full-length human SP-D are bronchoalveolar lavage (BAL) washings from normal or preferably patients suffering from alveolar proteinosis who overproduce SP-D in the lungs. Amniotic fluid collected at the term during parturition is another reasonable source. Here, we describe a simple and rapid method of purifying native SP-D away from SP-A which is also present in the same source. We also describe procedures of expressing and purifying a recombinant fragment of human SP-D (rhSP-D) comprising trimeric neck and carbohydrate recognition domains that has been shown to have therapeutic effects in murine models of allergy and infection. PMID:24218267

  10. Super-parameterization in GRAPES: The construction of SP-GRAPES and associated preliminary results

    NASA Astrophysics Data System (ADS)

    Zhu, Feng; Xu, Guoqiang; Zheng, Xiaohui; Wang, Yuhong

    2015-04-01

    Super-parameterization (SP) aims to explicitly represent deep convection within a coarse resolution global model by embedding a cloud resolving model (CRM) in each column of the mother model. For the first time, we implemented the SP in a mesoscale regional weather model, the Global/Regional Assimilation and PrEdiction System (GRAPES). The constructed SP-GRAPES uses a two-dimensional (2D) CRM in each grid column. A control and two SP simulations are conducted for the Beijing "7.21" heavy rainfall event to evaluate improvements in GRAPES using SP. The SP-run-I is a basic SP run delivering microphysics feedback only, whereas the SP-run-II delivers both microphysical and cloud fraction feedbacks. A comparison of the runs indicates that the SP-run-I has a slightly positive impact on the precipitation forecast than the control run. However, the inclusion of cloud fraction feedback leads to an evident overall improvement, particularly in terms of cloud fraction and 24-h cumulative precipitation. Although this is only a preliminary study using SP-GRAPES, we believe that it will provide considerable guidance for follow-up studies using SP in China.

  11. Nitrate levels modulate the abundance of Paracoccus sp. in a biofilm community.

    PubMed

    Singh, Shantanu; Nerurkar, Anuradha S; Srinandan, C S

    2015-06-01

    Conditions required to enhance a particular species efficient in degradative capabilities is very useful in wastewater treatment processes. Paracoccus sp. is known to efficiently reduce nitrogen oxides (NOx) due to the branched denitrification pathway. Individual-based simulations showed that the relative fitness of Paracoccus sp. to Pseudomonas sp. increased significantly with nitrate levels above 5 mM. Spatial structure of the biofilm showed substantially less nitrite levels in the areas of Paracoccus sp. dominance. The simulation was validated in a laboratory reactor harboring biofilm community by fluorescent in situ hybridization, which showed that increasing nitrate levels enhanced the abundance of Paracoccus sp. Different levels of NOx did not display any significant effect on biofilm formation of Paracoccus sp., unlike several other bacteria. This study shows that the attribute of Paracoccus sp. to tolerate and efficiently reduce NOx is conferring a fitness payoff to the organism at high concentrations of nitrate in a multispecies biofilm community. PMID:25838197

  12. Functional role of post-translational modifications of Sp1 in tumorigenesis

    PubMed Central

    2012-01-01

    Specific protein 1 (Sp1), the first transcription factor to be isolated, regulates the expression of numerous genes involved in cell proliferation, apoptosis, and differentiation. Recent studies found that an increase in Sp1 transcriptional activity is associated with the tumorigenesis. Moreover, post-translational modifications of Sp1, including glycosylation, phosphorylation, acetylation, sumoylation, ubiquitination, and methylation, regulate Sp1 transcriptional activity and modulate target gene expression by affecting its DNA binding activity, transactivation activity, or protein level. In addition, recent studies have investigated several compounds with anti-cancer activity that could inhibit Sp1 transcriptional activity. In this review, we describe the effect of various post-translational modifications on Sp1 transcriptional activity and discuss compounds that inhibit the activity of Sp1. PMID:23148884

  13. Cadmium-resistance in growing Rhodotorula sp. Y11.

    PubMed

    Li, ZhiJian; Yuan, HongLi; Hu, XueDa

    2008-03-01

    The uptake of cadmium and responses to this metal were studied in growing Rhodotorula sp. Y11. In presence of cadmium, Y11 showed a similar lag phase with decreased mu(max) in comparison to the control cultures in the absence of cadmium. Different changes in contents of cell elemental composition (carotenoids, total protein, total soluble sugar, and phosphate content) were observed under cadmium pressure. Growing Rhodotorula sp. Y11 took up cadmium in a biphasic mode, involving an initial energy-independent biosorption to the cell surface, followed by a slower energy-dependent intracellular accumulation. In the presence of metabolic inhibitors, intracellular cadmium uptake of growing Y11 was significantly influenced. PMID:17376676

  14. Cretevania bechlyi sp. nov., from Cretaceous Burmese amber (Hymenoptera: Evaniidae).

    PubMed

    Jennings, John T; Krogmann, Lars; Mew, Steven L

    2013-01-01

    The fossil evaniid wasp Cretevania bechlyi sp. nov., is described based on a well preserved female specimen from Cretaceous Burmese amber. The new species is placed in the genus Cretevania Rasnitsyn, 1975 based on the elongation of the mid and hind trochantellus, the fore wing venation (e.g. first marginal cell triangular and broad, 2m-cu absent, second sub-marginal cell separated from first discal cell), the shape of the petiole (subcylindrical with distal extension) and other distinct morphological features. Cretevania bechlyi sp. nov. differs from all previously described species in having just 10 flagellomeres (11 in other members of the genus) and in the presence of notauli (absent in other species). The new species represents the first species of Cretevania from Burmese amber and significantly expands the known morphological diversity of Mesozoic Evaniidae. PMID:24699575

  15. Biodegradation of 4-chloroindole by Exiguobacterium sp. PMA.

    PubMed

    Arora, Pankaj Kumar; Bae, Hanhong

    2015-03-01

    Exiguobacterium sp. PMA utilized 4-chloroindole as its sole source of carbon and energy. The effect of initial concentrations of substrate on the 4-chloroindole degradation was studied and observed that strain PMA was capable of degrading 4-chloroindole up to concentration of 0.5mM. The degradation pathway of 4-chloroindole was studied for Exiguobacterium sp. PMA based on metabolites identified by gas chromatography-mass spectrometry. 4-Chloroindole was initially dehalogenated to indole that was further degraded via isatin, anthranilic acid, and salicylic acid. The potential of strain PMA to degrade 4-chloroindole in soil was monitored using soil microcosms, and it was observed that the cells of strain PMA efficiently degraded 4-chloroindole in the soil. The results of microcosm studies show that strain PMA may be used for bioremediation of 4-chloroindole-contaminated sites. This is the first report of the bacterial degradation of 4-chloroindole. PMID:25463241

  16. Laboratory studies on biomachining of copper using Staphylococcus sp.

    PubMed

    Shikata, Shinji; Sreekumari, Kurissery R; Nandakumar, Kanavillil; Ozawa, Mazayoshi; Kikuchi, Yasushi

    2009-01-01

    The possibility of using bacteria to drill metallic surfaces has been demonstrated using Staphylococcus sp., a facultative anaerobic bacterium, isolated from corroded copper piping. The experiment involved exposure of copper coupons (25 mm x 15 mm x 3 mm) to a culture of Staphylococcus sp. for a maximum period of 7 days. Coupons exposed to sterile bacterial growth medium were used as controls. Exposed coupons were removed intermittently and observed microscopically for the extent of drilling. The total pit area and volume on these coupons were determined using image analysis. The results showed that both the biomachined area and volume increased with the duration of coupon exposure. In the drilling experiment, a copper thin film 2 microm thick was perforated by this bacterium within a period of 7 days. In conclusion, the results suggested that bacteria can be used as a tool for machining metallic surfaces. PMID:19440892

  17. Regioselective glucosylation of inositols catalyzed by Thermoanaerobacter sp. CGTase.

    PubMed

    Miranda-Molina, Alfonso; Marquina-Bahena, Silvia; López-Munguía, Agustín; Alvarez, Laura; Castillo, Edmundo

    2012-10-01

    Monoglucosylated products of L-chiro-, D-chiro-, muco-, and allo-inositol were synthesized by regioselective ?-D-glucosylation with cyclodextrin glucosyl transferase from Thermoanaerobacter sp. after hydrolysis of by products with Aspergillus niger glucoamylase. While the reactions carried out with D-chiro-, muco-, and allo-inositol resulted in the regioselective formation of monoglucosylated products, two products were obtained in the reaction with L-chiro-inositol. Through the structural characterization of the glucosylated inositols here we demonstrated that the selectivity observed in the glucosylation of several inositols by Thermoanaerobacter sp. CGTase, is analogous to the specificity observed for the glucosylation of ?-D-glucopyranose and equivalent glucosides. PMID:22940181

  18. Description of Goffartia phalacra n. sp. (Diplogastridae: Nematoda) from India

    PubMed Central

    Singh, Gaurav K.; Yousuf, Gazala; Kumar, Puneet; Ahmad, Irfan

    2014-01-01

    A new species, Goffartia phalacra n. sp. is described and illustrated. The body is thin and slender with L = 511 to 646 ?m; a = 37.1 to 47.4; b = 4.8 to 6; c = 2.6 to 4.8; c? = 13.6 to 32.8; V = 40% to 49% in females. Males are smaller but similar to females and the posterior region is strongly curved. The species is characterized by a tubular stoma, a smooth round lip region, anterior pharynx much smaller than posterior pharynx, two pairs of unicellular glands associated with the vagina, and males with a broad keel-shaped gubernaculum. G. phalacra n. sp. can be differentiated from all other species of the genus by its lip region and the structure of the gubernaculum. This is the first instance of a species of Goffartia occurring in a terrestrial habitat and the first report of a species from India. PMID:24987164

  19. Effect of temperature on ammonium removal in Scenedesmus sp.

    PubMed

    Ruiz-Martínez, A; Serralta, J; Seco, A; Ferrer, J

    2015-09-01

    The effect of temperature on microalgal ammonium uptake was investigated by carrying out four batch experiments in which a mixed culture of microalgae, composed mainly of Scenedesmus sp., was cultivated under different temperatures within the usual temperature working range in Mediterranean climate (15-34°C). Ammonium removal rates increased with temperature up to 26°C and stabilized thereafter. Ratkowsky and Cardinal temperatures models successfully reproduced the experimental data. Optimum (31.3°C), minimum (8.8°C) and maximum (46.1°C) temperatures for ammonium removal by Scenedesmus sp. under the studied conditions were obtained as model parameters. These temperature-related parameters constitute very useful information for designing and operating wastewater treatment systems using these microalgae. PMID:26027902

  20. SP-100 Ground Engineering Systems: Test facility control systems

    SciTech Connect

    Carlson, W.F.; Henshall, J.B.; Hampsten, K.L.

    1988-01-01

    One of the major objectives of the current phase of the SP-100 program is to demonstrate the performance of a full-scale, prototypic nuclear subsystem of a 100 kWe space nuclear power supply. Such a test is planned at the SP-100 Test Site located at US Department of Energy facilities near Richland, Washington. Considerations involved in selecting a design for the central, integrated facility control system which will control and monitor the performance of the nuclear subsystem and supporting facility systems are discussed. These considerations have been translated into the design of a modern, commerically available, microprocessor based control and monitoring system, using color graphics techniques for the operator interface.

  1. Siphateles (Gila) sp. and Catostomus sp. from the Pleistocene OIS-6 Lake Gale, Panamint Valley, Owens River system, California

    NASA Astrophysics Data System (ADS)

    Jayko, A. S.; Forester, R. M.; Smith, G. R.

    2014-12-01

    Panamint Valley lies within the Owens River system which linked southeastern Sierra Nevada basins between Mono Lake and Death Valley during glacial-pluvial times. Previous work indicates that late Pleistocene glacial-pluvial Lake Gale, Panamint Valley was an open system during OIS-6, a closed ground water supported shallow lake during OIS-4, and the terminal lake basin for the Owens River system during OIS-2. We here report the first occurrence of fossil fish from the Plio-Pleistocene Panamint basin. Fish remains are present in late Pleistocene OIS-6 nearshore deposits associated with a highstand that was spillway limited at Wingate Wash. The deposits contain small minnow-sized remains from both Siphateles or Gila sp. (chubs) and Catostomus sp. (suckers) from at least four locations widely dispersed in the basin. Siphateles or Gila sp. and Catostomus are indigenous to the Pleistocene and modern Owens River system, in particular to the historic Owens Lake area. Cyprinodon (pupfish) and Rhinichthys (dace) are known from the modern Amargosa River and from Plio-Pleistocene deposits in Death Valley to the east. The late Pleistocene OIS-6 to OIS-2 lacustrine and paleohydrologic record in Panamint basin is interpreted from ostracod assemblages, relative abundance of Artemia sp. pellets, shallow water indicators including tufa fragments, ruppia sp. fragments and the relative abundance of charophyte gyrogonites obtained from archived core, as well as faunal assemblages from paleoshoreline and nearshore deposits. The OIS-4 groundwater supported shallow saline lake had sufficiently low ratios of alkalinity to calcium (alk/Ca) to support the occurrence of exotic Elphidium sp. (?) foraminfera which are not observed in either OIS-2 or OIS-6 lacustrine deposits. The arrival of Owens River surface water into Panamint Basin during OIS-2 is recorded by the first appearance of the ostracod Limnocythere sappaensis at ~27 m depth in an ~100 m archived core (Smith and Pratt, 1957) which extends between OIS-5 and post OIS-2 based on based on proxy correlation with the marine oxygen isotope record.

  2. Power plant system assessment. Final report. SP100 Program

    Microsoft Academic Search

    R. V. Anderson; D. F. Atkins; D. S. Bost; B. Berman; D. A. Clinger; W. R. Determan; G. S. Drucker; L. E. Glasgow; J. A. Hartung; R. B. Harty

    1983-01-01

    The purpose of this assessment was to provide system-level insights into 100-kWe-class space reactor electric systems. Using these insights, Rockwell was to select and perform conceptual design studies on a ''most attractive'' system that met the preliminary design goals and requirements of the SP-100 Program. About 4 of the 6 months were used in the selection process. The remaining 2

  3. Phycocyanobilin:Ferredoxin Oxidoreductase of Anabaena sp. PCC 7120

    Microsoft Academic Search

    Nicole Frankenberg; J. Clark Lagarias

    In cyanobacteria, the biosynthesis of the phycobili- protein and phytochrome chromophore precursor phy- cocyanobilin is catalyzed by the ferredoxin-dependent enzyme phycocyanobilin:ferredoxin oxidoreductase (PcyA), which mediates an atypical four-electron reduc- tion of biliverdin IX. Here we describe the expression, affinity purification, and biochemical characterization of recombinant PcyA from Anabaena sp. PCC 7120. A monomeric protein with a native Mr of 30,400

  4. Production of cholesterol oxidase by a newly isolated Rhodococcus sp

    Microsoft Academic Search

    M. Tabatabaei Yazdi; F. Malekzadeh; Gh. Zarrini; M. A. Faramarzi; N. Kamranpour; Sh. Khaleghparast

    2001-01-01

    Fifteen strains of microorganisms with ability to degrade cholesterol were isolated. Among them a Gram-positive, non-motile, non-sporing bacterium with meso-DAP in the cell wall and with a rod-coccus cycle showed the highest ability for cholesterol degradation. It was identified as Rhodococcus sp. strain 2C and was deposited by code 1633 in Persian type culture collection (PTCC). This strain was able

  5. Biodegradation of 4-nitrotoluene by pseudomonas sp. strain 4NT

    SciTech Connect

    Haigler, B.E.; Spain, J.C. (Tyndall Air Force Base, FL (United States))

    1993-07-01

    Nitroaromatic compounds, common intermediates or by-products in synthesis of dyes, solvents, and explosives, has resulting in their emergence as environmental contaminants. Bacterial strains able to degrade 4-nitrotoluene (4-NT) have been isolated. The present study reports the complete degradative pathway of Pseudomonas sp. strain 4NT that uses 4-NT as a sole source of carbon, nitrogen, and energy. 33 refs., 2 figs., 3 tabs.

  6. Characterization of Clostridium sp. RKD producing botulinum-like neurotoxin

    Microsoft Academic Search

    Aparna Dixit; Ram Kumar Dhaked; Syed Imteyaz Alam; Lokendra Singh

    2005-01-01

    A Gram positive, motile, rod-shaped, strictly anaerobic bacterium isolated from intestine of decaying fish was identified as Clostridium sp. RKD and produced a botulinum type B-like neurotoxin as suggested by mouse bioassay and protection with anti botulinum antibodies. The neurotoxicity was functionally characterized by the phrenic nerve hemi-diaphragm assay. Phylogenetic analysis based on 16S rDNA sequence, placed it at a

  7. Docosahexaenoic acid (DHA) production by Thraustochytrium sp. ATCC 20892

    Microsoft Academic Search

    A. Singh; S. Wilson; O. P. Ward

    1996-01-01

    Thraustochytrium sp. ATCC 20892 produced high yields of docosahexaenoic acid (DHA), more than four other strains of Thraustochytrium and Schizochytrium tested, but insignificant amounts of other polyunsaturated fatty acids. Glucose and sodium glutamate were the preferred carbon and nitrogen sources, respectively, and the optimum conditions for growth and DHA production were pH 7.0 at 25°C with 40 g glucose 1-1

  8. Comparison of 128-SP-QAM with PM-16-QAM.

    PubMed

    Sjödin, Martin; Johannisson, Pontus; Li, Jianqiang; Agrell, Erik; Andrekson, Peter A; Karlsson, Magnus

    2012-04-01

    In this paper we investigate an interesting modulation format for fiber optic communications, set-partitioning 128 polarization-multiplexed 16-QAM (128-SP-QAM), which consists of the symbols with even parity from the symbol alphabet of polarization-multiplexed 16-QAM (PM-16-QAM). We compare 128-SP-QAM and PM-16-QAM using numerical simulations in long-haul transmission scenarios at bit rates of 112 Gbit/s and 224 Gbit/s, and at the same symbol rates (14 and 28 Gbaud). The transmission link is made up of standard single-mode fiber with 60, 80 or 100 km amplifier spacing and both single channel and WDM transmission (25- and 50 GHz-spaced) is investigated. The results show that 128-SP-QAM achieves more than 40% increase in transmission reach compared to PM-16-QAM at the same data rate for all cases studied for a bit error rate of 10?³. In addition, we find that in single channel transmission there is, as expected, an advantage in terms of transmission distance when using a data rate of 112 Gbit/s as compared to 224 Gbit/s. However, when comparing the two different WDM systems with the same aggregate data rates, the reach is similar due to the smaller impact of nonlinear crosstalk between the WDM channels in the systems with 50 GHz spacing. We also discuss decoding and phase estimation of 128-SP-QAM and implement differential coding, which avoids error bursts due to cycle slips in the phase estimation. Simulations including laser phase noise show that the phase noise tolerance is similar for the two formats, with 0.5 dB OSNR penalty compared to the case with zero phase noise for a laser linewidth to symbol rate ratio of 10??. PMID:22513547

  9. Cytochalasin derivatives from a jellyfish-derived fungus Phoma sp.

    PubMed

    Kim, Eun La; Wang, Haibo; Park, Ju Hee; Hong, Jongki; Choi, Jae Sue; Im, Dong Soon; Chung, Hae Young; Jung, Jee H

    2015-05-15

    Four new cytochalasin derivatives (1-4), together with proxiphomin (5), were isolated from a jellyfish-derived fungus Phoma sp. The planar structures and relative stereochemistry were established by analysis of 1D and 2D NMR data. The absolute configuration was defined by the modified Mosher's method. The compounds showed moderate cytotoxicity against a small panel of human solid tumor cell lines (A549, KB, and HCT116). PMID:25881821

  10. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov. and Candida manassasensis sp. nov., novel yeasts associated with wood-ingesting insects, and their ecological and biofuel implications.

    PubMed

    Suh, Sung-Oui; Houseknecht, Janice L; Gujjari, Pushpa; Zhou, Jianlong J

    2013-11-01

    During a survey of yeasts associated with wood-ingesting insects, 69 strains in the Scheffersomyces clade and related taxa were isolated from passalid and tenebrionid beetles and the decayed wood inhabited by them. The majority of these yeasts was found to be capable of fermenting xylose, and was recognized as Scheffersomyces stipitis or its close relative Scheffersomyces illinoinensis, which are known to be associated with wood-decaying beetles and rotten wood. Yeasts in 'Scheffersomyces' (?=?Candida) ergatensis and 'Scheffersomyces' (?=?Candida) coipomoensis were also frequently isolated. The remaining six strains were identified as representing four novel species in the genera Scheffersomyces and Candida based on multilocus sequence analyses of nuclear rRNA genes and four protein-coding genes, as well as other taxonomic characteristics. Two xylose-fermenting species, Scheffersomyces parashehatae f.a., sp. nov. (type strain ATCC MYA-4653(T)?=?CBS 12535(T)?=?EH045(T); MycoBank MB805440) and Scheffersomyces xylosifermentans f.a., sp. nov. (type strain ATCC MYA-4859(T)?=?CBS 12540(T)?=?MY10-052(T); MycoBank MB805441), formed a clade with Scheffersomyces shehatae and related Scheffersomyces species. Interestingly, S. xylosifermentans can survive at 40 °C, which is a rare property among xylose-fermenting yeasts. Candida broadrunensis sp. nov. (type strain ATCC MYA-4650(T)?=?CBS 11838(T)?=?EH019(T); MycoBank MB805442) is a sister taxon of C. ergatensis, while Candida manassasensis sp. nov. (type strain ATCC MYA-4652(T)?=?CBS 12534(T)?=?EH030(T); MycoBank MB805443) is closely related to Candida palmioleophila in the Candida glaebosa clade. The multilocus DNA sequence comparisons in this study suggest that the genus Scheffersomyces needs to be circumscribed to the species near S. stipitis (type species) and S. shehatae that can be characterized by the ability to ferment xylose. PMID:24014624

  11. Conceptual arcjet system design considerations for the SP100 mission

    Microsoft Academic Search

    Zafran

    1989-01-01

    Conceptual spacecraft design considerations for using 30-kW class arcjet engines as an active load for a 100-kWe SP-100 space nuclear reactor power system flight experiment are discussed. These design considerations involve configuration trades; interfaces with power, attitude and velocity control, thermal management, orbit insertion and reaction control propulsion systems; imposed environments concerned with electromagnetic noise, survivability, and ammonia exhaust plumes;

  12. Natural transformation of Thermotoga sp. strain RQ7

    PubMed Central

    2014-01-01

    Background Thermotoga species are organisms of enormous interest from a biotechnological as well as evolutionary point of view. Genetic modifications of Thermotoga spp. are often desired in order to fully release their multifarious potentials. Effective transformation of recombinant DNA into these bacteria constitutes a critical step of such efforts. This study aims to establish natural competency in Thermotoga spp. and to provide a convenient method to transform these organisms. Results Foreign DNA was found to be relatively stable in the supernatant of a Thermotoga culture for up to 6 hours. Adding donor DNA to T. sp. strain RQ7 at its early exponential growth phase (OD600 0.18?~?0.20) resulted in direct acquisition of the DNA by the cells. Both T. neapolitana chromosomal DNA and Thermotoga-E. coli shuttle vectors effectively transformed T. sp. strain RQ7, rendering the cells resistance to kanamycin. The kan gene carried by the shuttle vector pDH10 was detected by PCR from the plasmid extract of the transformants, and the amplicons were verified by restriction digestions. A procedure for natural transformation of Thermotoga spp. was established and optimized. With the optimized method, T. sp. strain RQ7 sustained a transformation frequency in the order of 10-7 with both genomic and plasmid DNA. Conclusions T. sp. strain RQ7 cells are naturally transformable during their early exponential phase. They acquire DNA from both closely and distantly related species. Both chromosomal DNA and plasmid DNA serve as suitable substrates for transformation. Our findings lend a convenient technical tool for the genetic engineering of Thermotoga spp. PMID:24884561

  13. Cytotoxic Sesterterpenoids Isolated from the Marine Sponge Scalarispongia sp.

    PubMed Central

    Lee, Yeon-Ju; Lee, Jeong-Woo; Lee, Dong-Geun; Lee, Hyi-Seung; Kang, Jong Soon; Yun, Jieun

    2014-01-01

    Eight scalarane sesterterpenoids, including four new compounds, were isolated from the marine sponge Scalarispongia sp. The structures of the new compounds were elucidated by 2D-NMR and HRMS analyses. All of the isolated compounds, with the exception of 16-O-deacetyl-12,16-epi-scalarolbutanolide, showed significant in vitro cytotoxicity (GI50 values down to 5.2 ?M) against six human cancer cell lines. PMID:25375188

  14. Anagnorisma chamrani sp. n. (Lepidoptera, Noctuidae) from Iran

    PubMed Central

    Gyulai, Peter; Rabieh, Mohammad Mahdi; Seraj, Ali Asghar; Ronkay, Laslo; Esfandiari, Mehdi

    2013-01-01

    Abstract A new Anagnorisma species, Anagnorisma chamrani sp. n., is described from Binaloud Mountains of Khorasan-e-Razavi province in north-eastern Iran, and compared with its sister species, Anagnorisma eucratides (Boursin, 1960). The adults, and male and female genitalia of both species are illustrated in 11 figures. The genus Anagnorisma is recorded for the first time for the fauna of Iran. PMID:23950668

  15. Nutritional Requirements of Methanosarcina sp. Strain TM-1.

    PubMed

    Murray, P A; Zinder, S H

    1985-07-01

    Methanosarcina sp. strain TM-1, an acetotrophic, thermophilic methanogen isolated from an anaerobic sludge digestor, was originally reported to require an anaerobic sludge supernatant for growth. It was found that the sludge supernatant could be replaced with yeast extract (1 g/liter), 6 mM bicarbonate-30% CO(2), and trace metals, with a doubling time on methanol of 14 h. For growth on either methanol or acetate, yeast extract could be replaced with CaCl(2) . 2H(2)O (13.6 muM minimum) and the vitamin p-aminobenzoic acid (PABA, ca. 3 nM minimum), with a doubling time on methanol of 8 to 9 h. Filter-sterilized folic acid at 0.3 muM could not replace PABA. The antimetabolite sulfanilamide (20 mM) inhibited growth of and methanogenesis by Methanosarcina sp. strain TM-1, and this inhibition was reversed by the addition of 0.3 muM PABA. When a defined medium buffered with 20 mM N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid was used, it was shown that Methanosarcina sp. strain TM-1 required 6 mM bicarbonate-30% CO(2) for optimal growth and methanogenesis from methanol. Cells growing on acetate were less dependent on bicarbonate-CO(2). When we used a defined medium in which the only organic compounds present were methanol or acetate, nitrilotriacetic acid (0.2 mM), and PABA, it was possible to limit batch cultures of Methanosarcina sp. strain TM-1 for nitrogen at NH(4) concentrations at or below 2.0 mM, in marked contrast with Methanosarcina barkeri 227, which fixes dinitrogen when grown under NH(4) limitation. PMID:16346841

  16. Larinus berti sp. n. (Coleoptera, Curculionidae, Lixinae) from North Africa

    PubMed Central

    Gültekin, Levent; Alonso-Zarazaga, Miguel A.

    2013-01-01

    Abstract A new species, Larinus berti sp. n. is described from Morocco and assigned to subgenus Cryphopus Petri, 1907 (Curculionidae: Lixinae; Lixini). Diagnostic characters of the new species are large size, elongate-ovate body, bisulcate sub-quadrangular rostrum, triangularly raised dorsum of rostrum, flat subgena and submentum, Y-shaped apodeme of sternite VIII of female and thin nodulus of spermatheca. PMID:24194653

  17. Ultrastructure of Bonamia sp. in Ostrea chilensis in Chile.

    PubMed

    Lohrmann, K B; Hine, P M; Campalans, M

    2009-07-23

    Oyster Ostrea chilensis samples were collected from Quihua Island, Chile, in December 2003 and February 2005, and examined in May 2004, and March, April and July 2005, for an ultrastructural comparison of the Chilean Bonamia sp. with other Bonamia spp. Only uni-nucleate stages were encountered, except in the July sample. The Chilean parasite differs from B. perspora in the apparent lack of a plasmodial stage and of sporulation. It resembles B. ostreae in size, the low number of mitochondrial profiles, and the prevalence and mean number of lipid droplets. It differs from B. ostreae in the greater prevalence of nuclear membrane-bound Golgi (NM-BG), associated haplosporogenesis, and smaller size of haplosporosomes. The Chilean Bonamia sp. resembles B. exitiosa in the number of haplosporosomes, prevalence of lipid droplets, anastomosing endoplasmic reticulum and NM-BG, presence of circles of smooth endoplasmic reticulum (sER), confronting cisternae (CC), and cylindrical CC (CCC). It also appears to have a similar developmental cycle to B. exitiosa with larger forms occurring in winter (August). The circles of sER, CC, and CCC have only been reported from B. exitiosa, and it appears that Chilean Bonamia sp. and B. exitiosa are more closely related than they are to B. perspora or B. ostreae. Similarities in ultrastructure and developmental stages between New Zealand and Chilean parasites suggest that the 2 species are related, and that the Chilean Bonamia sp. is either B. exitiosa, a sub-species of B. exitiosa, or a separate species closely related to B. exitiosa. PMID:19750808

  18. Homalomena propinqua (Araceae) is Pollinated by Parastasia sp. (Scarabaeidae)

    NSDL National Science Digital Library

    2004-03-09

    Beetles are important pollinators in a lowland dipterocarp forest in Southeast Asia. Most of canopy and emergent dipterocarps are pollinated by beetles feeding on floral tissues. Some understory trees and forest floor herbs are pollinated by different types of beetles. Homalomena propinqua (Araceae) is pollinated by two specias of beetles. One of them, Parastasia sp. (Scarabaeidae), stays inside the spathe chamber and feeds on staminodes.

  19. A New Actinomycete Species, Nocardiopsis lucentensis sp. nov

    Microsoft Academic Search

    A. F. YASSIN; E. A. GALINSKI; A. WOHLFARTH; K.-D. JAHNKE; K. P. SCHAAL; H. G. TRUPER

    A new species of the genus Nocardiopsis, for which we propose the name Nocardiopsis lucentensis sp. nov. (type strain, strain DSM 44048), was isolated from a salt marsh soil sample near Alicante, Spain. Whole-cell hydrolysates contain the meso isomer of diaminopimelic acid and no characteristic sugar; thus, the cell wall composition is type 111. Menaquinone MK-lO(H,) is the major menaquinone,

  20. PREVALENCE OF BRUCELLA SP. ANTIBODIES IN FERAL SWINE IN FLORIDA

    Microsoft Academic Search

    M. L. van der Leek; H. N. Becker; P. Humphrey; C. L Adams; R. C. Belden; W. B. Frankenberger; P. L. Nicoletti

    Serum samples collected from feral swine (Sus scrofa) throughout Florida (USA) from 1974 to 1989 were tested for antibodies to Brucella sp. by the card test, the standard tube test, the rivanol test or the complement fixation test. Seropositive swine were detected at six of 18 sites with a composite prevalence of 23.4% (238 of 1,015 samples; range = 5.5%

  1. Effect of hydrophobic surfactant proteins SP-B and SP-C on phospholipid monolayers. Protein structure studied using 2D IR and beta correlation analysis.

    PubMed Central

    Shanmukh, Saratchandra; Howell, Phillip; Baatz, John E; Dluhy, Richard A

    2002-01-01

    We have applied two-dimensional infrared (2D IR) and betanu correlation spectroscopy to in-situ IR spectroscopy of pulmonary surfactant proteins SP-B and SP-C in lipid-protein monolayers at the air-water interface. For both SP-B and SP-C, a statistical windowed autocorrelation method identified two separate surface pressure regions that contained maximum amide I intensity changes: 4-25 mN/m and 25-40 mN/m. For SP-C, 2D IR and betanu correlation analyses of these regions indicated that SP-C adopts a variety of secondary structure conformations, including alpha-helix, beta-sheet, and an intermolecular aggregation of extended beta-sheet structure. The main alpha-helix band split into two peaks at high surface pressures, indicative of two different helix conformations. At low surface pressures, all conformations of the SP-C molecule reacted identically to increasing surface pressure and reoriented in phase with each other. Above 25 mN/m, however, the increasing surface pressure selectively affected the coexisting protein conformations, leading to an independent reorientation of the protein conformations. The asynchronous 2D IR spectrum of SP-B showed the presence of two alpha-helix components, consistent with two separate populations of alpha-helix in SP-B-a hydrophobic fraction associated with the lipid chains and a hydrophilic fraction parallel to the membrane surface. The distribution of correlation intensity between the two alpha-helix cross peaks indicated that the more hydrophobic helix fraction predominates at low surface pressures whereas the more hydrophilic helix fraction predominates at high surface pressures. The different SP-B secondary structures reacted identically to increasing surface pressure, leading to a reorientation of all SP-B subunits in phase with one another. PMID:12324430

  2. Purification and characterization of chitinase from Paenibacillus sp. D1.

    PubMed

    Singh, Anil Kumar; Chhatpar, Hari S

    2011-05-01

    A 56.56-kDa extracellular chitinase from Paenibacillus sp. D1 was purified to 52.3-fold by ion exchange chromatography using SP Sepharose. Maximum enzyme activity was recorded at pH 5.0 and 50 °C. MALDI-LC-MS/MS analysis identified the purified enzyme as chitinase with 60% similarity to chitinase Chi55 of Paenibacillus ehimensis. The activation energy (E (a)) for chitin hydrolysis and temperature quotient (Q (10)) at optimum temperature was found to be 19.14 kJ/mol and 1.25, respectively. Determination of kinetic constants k (m), V (max), k (cat), and k (cat)/k (m) and thermodynamic parameters ?H*, ?S*, ?G*, ?G*(E-S), and ?G*(E-T) revealed high affinity of the enzyme for chitin. The enzyme exhibited higher stability in presence of commonly used protectant fungicides Captan, Carbendazim, and Mancozeb compared to control as reflected from the t (1/2) values suggesting its applicability in integrated pest management for control of soil-borne fungal phytopathogens. The order of stability of chitinase in presence of fungicides at 80 °C as revealed from t (1/2) values and thermodynamic parameters E (a(d)) (activation energy for irreversible deactivation), ?H*, ?G*, and ?S* was: Captan > Carbendazim > Mancozeb > control. The present study is the first report on thermodynamic and kinetic characterization of chitinase from Paenibacillus sp. D1. PMID:21049291

  3. Coupling Algorithm for Sp(3 , R) Irreducible Representations

    NASA Astrophysics Data System (ADS)

    St. Germaine-Fuller, James F.; McCoy, Anna E.; Caprio, Mark A.

    2015-04-01

    The nuclear symplectic model based on Sp(3 , R) - the smallest algebra that contains both the shell model Hamiltonian and the rotor algebra - connects the microscopic shell model to collective rotational behavior and naturally extends the Elliot SU(3) model to multiple shells. However, Sp(3 , R) is only an approximate symmetry of the nucleus which can be broken by spin-orbit interactions, tensor force interactions, and pairing. The Hamiltonians in most physical situations will break Sp(3 , R) symmetry, causing their eigenstates to become linear combinations of symplectic irreducible representations (irreps). Calculations with those eigenstates will then involve multiple irreps. We report a computer algorithm for enumerating the irreps that arise from the coupling of two symplectice irreps and evaluating their multiplicities in the product. This should assist in performing such multi-irrep calculations and facilitate computing symplectic coupling coefficients. Supported by the US NSF under Grant NSF-PHY05-52843, the US DOE under Grant DE-FG02-95ER-40934, and the Research Corporation for Science Advancement under a Cottrell Scholar Award.

  4. Hydrothermal acid treatment for sugar extraction from Golenkinia sp.

    PubMed

    Choi, Sun-A; Choi, Won-Il; Lee, Jin-Suk; Kim, Seung Wook; Lee, Gye-An; Yun, Jihyun; Park, Ji-Yeon

    2015-08-01

    In this study, hydrothermal acid treatment for efficient recovery of sugar from Golenkinia sp. was investigated. The initial glucose and XMG (xylose, mannose, and galactose) contents of a prepared Golenkinia sp. solution (40g/L) were 15.05 and 5.24g/L, respectively. The microalgal cell walls were hydrolyzed, for sugar recovery, by enzymatic saccharification and/or hydrothermal acid treatment. Among the various hydrothermal acid treatment conditions, the most optimal were the 2.0% H2SO4 concentration at 150°C for 15min, under which the glucose- and XMG-extraction yields were 71.7% and 64.9%, respectively. By pH 4.8, 50°C enzymatic hydrolysis after optimal hydrothermal acid treatment, the glucose- and XMG-extraction yields were additionally increased by 8.3% and 0.8%, respectively. After hydrothermal acid treatment, the combination with the enzymatic hydrolysis process improved the total sugar yield of Golenkinia sp. to 75.4%. PMID:25976916

  5. Identification of Arthrobacter oxydans, Arthrobacter luteolus sp. nov., and Arthrobacter albus sp. nov., Isolated from Human Clinical Specimens

    PubMed Central

    Wauters, Georges; Charlier, Jacqueline; Janssens, Michèle; Delmée, Michel

    2000-01-01

    Five Arthrobacter isolates from clinical specimens were studied by phenotypic, chemotaxonomic, and genetic characterization. Two strains had characteristics consistent with those of Arthrobacter oxydans. One strain was related to A. citreus; however, DNA-DNA hybridization and phenotypic characteristics indicated that this strain belongs to a new species, for which the name Arthrobacter luteolus sp. nov. is proposed. Two strains were closely related to A. cumminsii by 16S rRNA gene sequencing, but DNA-DNA hybridization, peptidoglycan type, and some phenotypic features indicated that they should be assigned to a new species, for which the name Arthrobacter albus sp. nov. is proposed. The type strain of A. luteolus is CF25 (DSM 13067). The type strain of A. albus is CF43 (DSM 13068). PMID:10835019

  6. Iron-Catalyzed Directed C(sp(2))-H and C(sp(3))-H Functionalization with Trimethylaluminum.

    PubMed

    Shang, Rui; Ilies, Laurean; Nakamura, Eiichi

    2015-06-24

    Conversion of a C(sp(2))-H or C(sp(3))-H bond to the corresponding C-Me bond can be achieved by using AlMe3 or its air-stable diamine complex in the presence of catalytic amounts of an inorganic iron(III) salt and a diphosphine along with 2,3-dichlorobutane as a stoichiometric oxidant. The reaction is applicable to a variety of amide substrates bearing a picolinoyl or 8-aminoquinolyl directing group, enabling methylation of a variety of (hetero)aryl, alkenyl, and alkyl amides. The use of the mild aluminum reagent prevents undesired reduction of iron and allows the reaction to proceed with catalyst turnover numbers as high as 6500. PMID:26061014

  7. Isolation and description of a stable carbazole-degrading microbial consortium consisting of Chryseobacterium sp. NCY and Achromobacter sp. NCW.

    PubMed

    Guo, Weiqiang; Li, Daping; Tao, Yong; Gao, Ping; Hu, Jijun

    2008-09-01

    A stable microbial consortium, separated from a refinery wastewater sample, was able to utilize carbazole as the sole source of carbon, nitrogen, and energy, and liberated ammonia from excess nitrogen. Two bacterial strains (NCY and NCW) were isolated from the microbial consortium using a nutrient agar plate. Based on the 16S rDNA sequence analysis, the two bacteria were identified as Chryseobacterium sp. NCY and Achromobacter sp. NCW, respectively. No intermediates of carbazole degradation were detected by high-performance liquid chromatography. The substrate specificity assay showed that the consortium could utilize compounds similar to carbazole, such as phenanthrene, naphthalene, and imidazole. Neither the pure strain NCY nor NCW could degrade carbazole after domestication for several times. It was suggested that the two bacteria formed a microbial consortium capable of metabolizing carbazole. PMID:18584242

  8. Integrated Bacillus sp. immobilized cell reactor and Synechocystis sp. algal reactor for the treatment of tannery wastewater.

    PubMed

    Sekaran, G; Karthikeyan, S; Nagalakshmi, C; Mandal, A B

    2013-01-01

    The wastewater discharged from leather industries lack biodegradability due to the presence of xenobiotic compounds. The primary clarification and aerobic treatment in Bacillus sp. immobilized Chemo Autotrophic Activated Carbon Oxidation (CAACO) reactor removed considerable amount of pollution parameters. The residual untreated organics in the wastewater was further treated in algal batch reactor inoculated with Synechocystis sp. Sodium nitrate, K(2)HPO(4), MgSO(4).7H(2)O, NH(4)Cl, CaCl(2)·2H(2)O, FeCl(3) (anhydrous), and thiamine hydrochloride, rice husk based activated carbon (RHAC), immobilization of Bacillus sp. in mesoporous activated carbon, sand filter of dimensions diameter, 6 cm and height, 30 cm; and the CAACO reactor of dimensions diameter, 5.5 cm and height, 30 cm with total volume 720 ml, and working volume of 356 ml. In the present investigation, the CAACO treated tannery wastewater was applied to Synechocystis sp. inoculated algal batch reactor of hydraulic residence time 24 h. The BOD(5), COD, and TOC of treated wastewater from algal batch reactor were 20 ± 7, 167 ± 29, and 78 ± 16 mg/l respectively. The integrated CAACO system and Algal batch reactor was operated for 30 days and they accomplished a cumulative removal of BOD(5),COD, TOC, VFA and sulphide as 98 %, 95 %, 93 %, 86 %, and 100 %, respectively. The biokinetic constants for the growth of algae in the batch reactor were specific growth rate, 0.095(day(-1)) and yield coefficient, 3.15 mg of algal biomass/mg of COD destructed. The degradation of xenobiotic compounds in the algal batch reactor was confirmed through HPLC and FT-IR techniques. The integrated CAACO-Algal reactor system established a credible reduction in pollution parameters in the tannery wastewater. The removal mechanism is mainly due to co-metabolism between algae and bacterial species and the organics were completely metabolized rather than by adsorption. PMID:22528997

  9. Sulfurospirillum barnesii sp. nov. and Sulfurospirillum arsenophilum sp. nov., new members of the Sulfurospirillum clade of the ?-Proteobacteria

    USGS Publications Warehouse

    Stolz, J.F.; Ellis, D.J.; Blum, J.S.; Ahmann, D.; Lovley, D.R.; Oremland, R.S.

    1999-01-01

    Two strains of dissimilatory arsenate-reducing vibrio-shaped bacteria are assigned to the genus Sulfurospirillum. These two new species, Sulfurospirillum barnesii strain SES-3(T) and Sulfurospirillum arsenophilum strain MIT-13(T), in addition to Sulfurospirillum sp. SM-5, two strains of Sulfurospirillum deleyianum, and Sulfurospirillum arcachonense, form a distinct clade within the ?? subclass of the Proteobacteria based on 16S rRNA analysis.

  10. Thermococcus paralvinellae sp. nov. and Thermococcus cleftensis sp. nov. of hyperthermophilic heterotrophs from deep-sea hydrothermal vents.

    PubMed

    Hensley, Sarah A; Jung, Jong-Hyun; Park, Cheon-Seok; Holden, James F

    2014-11-01

    Two heterotrophic hyperthermophilic strains, ES1(T) and CL1(T), were isolated from Paralvinella sp. polychaete worms collected from active hydrothermal vent chimneys in the north-eastern Pacific Ocean. Both were obligately anaerobic and produced H2S in the presence of elemental sulfur and H2. Complete genome sequences are available for both strains. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strains are more than 97% similar to most other species of the genus Thermococcus. Therefore, overall genome relatedness index analyses were performed to establish that these strains are novel species. For each analysis, strain ES1(T) was determined to be most similar to Thermococcus barophilus MP(T), while strain CL1(T) was determined to be most similar to Thermococcus sp. 4557. The average nucleotide identity scores for these strains were 84% for strain ES1(T) and 81% for strain CL1(T), genome-to-genome direct comparison scores were 23% for strain ES1(T) and 47% for strain CL1(T), and the species identification scores were 89% for strain ES1(T) and 88% for strain CL1(T). For each analysis, strains ES1(T) and CL1(T) were below the species delineation cut-off. Therefore, based on their whole genome sequences, strains ES1(T) and CL1(T) are suggested to represent novel species of the genus Thermococcus for which the names Thermococcus paralvinellae sp. nov. and Thermococcus cleftensis sp. nov. are proposed, respectively. The type strains are ES1(T) (?=DSM 27261(T)?=KACC 17923(T)) and CL1(T) (?=DSM 27260(T)?=KACC 17922(T)). PMID:25082851

  11. Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)

    PubMed Central

    Olmstead, Ian L. D.; Bergamin, Amanda; Shears, Melanie J.; Dias, Daniel A.; Kentish, Sandra E.; Scales, Peter J.; Botté, Cyrille Y.; Callahan, Damien L.

    2014-01-01

    Many species of microalgae produce greatly enhanced amounts of triacylglycerides (TAGs), the key product for biodiesel production, in response to specific environmental stresses. Improvement of TAG production by microalgae through optimization of growth regimes is of great interest. This relies on understanding microalgal lipid metabolism in relation to stress response in particular the deprivation of nutrients that can induce enhanced TAG synthesis. In this study, a detailed investigation of changes in lipid composition in Chlorella sp. and Nannochloropsis sp. in response to nitrogen deprivation (N-deprivation) was performed to provide novel mechanistic insights into the lipidome during stress. As expected, an increase in TAGs and an overall decrease in polar lipids were observed. However, while most membrane lipid classes (phosphoglycerolipids and glycolipids) were found to decrease, the non-nitrogen containing phosphatidylglycerol levels increased considerably in both algae from initially low levels. Of particular significance, it was observed that the acyl composition of TAGs in Nannochloropsis sp. remain relatively constant, whereas Chlorella sp. showed greater variability following N-deprivation. In both algae the overall fatty acid profiles of the polar lipid classes were largely unaffected by N-deprivation, suggesting a specific FA profile for each compartment is maintained to enable continued function despite considerable reductions in the amount of these lipids. The changes observed in the overall fatty acid profile were due primarily to the decrease in proportion of polar lipids to TAGs. This study provides the most detailed lipidomic information on two different microalgae with utility in biodiesel production and nutraceutical industries and proposes the mechanisms for this rearrangement. This research also highlights the usefulness of the latest MS-based approaches for microalgae lipid research. PMID:25171084

  12. [Prevalence of Cyclospora sp., Cryptosporidium sp, microsporidia and fecal coliform determination in fresh fruit and vegetables consumed in Costa Rica].

    PubMed

    Calvo, Melvin; Carazo, Melissa; Arias, Maria Laura; Chaves, Carolina; Monge, Rafael; Chinchilla, Misael

    2004-12-01

    The presence of Cyclospora sp., Cryptosporidium sp. and microsporidia and the levels of fecal coliforms were determined in lettuce, parsley, cilantro, strawberries and blackberries acquired in local agricultural markets of the Central Valley of Costa Rica, in order to establish the possible transmission risk of these microorganisms and other pathogens from the consumption of these raw products. During the second semester of 2001 and the first of 2002, 50 different samples of each product, 25 taken in the dry season and 25 in the rainy season and coming from five different local agricultural markets were evaluated. The fecal coliforms count was done according to the technique recommended by Vanderzant & Splittstoesser. The parasite determination was done using Zielh Nielsen and Weber staining techniques from a sediment obtained through the rinse of the mentioned products, using sterile peptonated water 0.1% and centrifuging at 900 G for 15 min. One hundred per cent of vegetable samples had fecal coliforms and the greatest prevalence was obtained during the rainy season. Although all vegetables presented fecal coliforms in high concentrations, lettuce and cilantro presented statistical difference between rainy and dry season, being greater during the rainy season. Fecal coliforms were not detected in strawberries and blackberries probablydue to its low pH. All products evaluated presented, at least once, Cryptosporidium sp., Cyclospora sp. and microsporidia, showing the risk they represent to Public Health. Cryptosporidium was present in all products but strawberries. Microsporidia was present in all products except blackberries and Cyclospora was only isolated from lettuce during the dry season. These results show the importance of introducing in the country Good Agricultural Practices, especially due to the resistance of Cryptosporidium and Cyclospora to disinfecting agents. PMID:15969268

  13. A functional comparison of the venom of three Australian jellyfish— Chironex fleckeri, Chiropsalmus sp., and Carybdea xaymacana—on cytosolic Ca 2+, haemolysis and Artemia sp. lethality

    Microsoft Academic Search

    Paul M. Bailey; Anthony J. Bakker; Jamie E. Seymour; Jacqueline A. Wilce

    2005-01-01

    Cnidarian venoms produce a wide spectrum of envenoming syndromes in humans ranging from minor local irritation to death. Here, the effects of Chironex fleckeri, Chiropsalmus sp., and Carybdea xaymacana venoms on ventricular myocyte cytosolic Ca2+, haemolysis and Artemia sp. lethality are compared for the first time. All three venoms caused a large, irreversible elevation of cytosolic Ca2+ in myocytes as

  14. Genome Sequences of the Lignin-Degrading Pseudomonas sp. Strain YS-1p and Rhizobium sp. Strain YS-1r Isolated from Decaying Wood

    PubMed Central

    Prabhakaran, Madhu; Couger, Matthew B.; Jackson, Colin A.; Weirick, Tyler

    2015-01-01

    Pseudomonas sp. strain YS-1p and Rhizobium sp. strain YS-1r were isolated from a lignin-degrading enrichment culture. The isolates degraded lignin-derived monomers, dimers, alkali lignin, and, to a smaller extent (3% to 5%), lignin in switch grass and alfalfa. Genome analysis revealed the presence of a variety of lignin-degrading genes. PMID:25744986

  15. Babesia bicornis sp. nov. and Theileria bicornis sp. nov.: Tick-Borne Parasites Associated with Mortality in the Black Rhinoceros (Diceros bicornis)

    PubMed Central

    Nijhof, Ard M.; Penzhorn, Banie L.; Lynen, Godelieve; Mollel, Johnson O.; Morkel, Pete; Bekker, Cornelis P. J.; Jongejan, Frans

    2003-01-01

    A novel Babesia species, designated Babesia bicornis sp. nov., was identified in three black rhinoceroses (Diceros bicornis) that died in wildlife areas in Tanzania and South Africa. Screening of black rhinoceroses in South Africa revealed, in addition to B. bicornis, a second parasite, designated Theileria bicornis sp. nov. PMID:12734294

  16. Gap junctional communication modulates gene transcription by altering the recruitment of Sp1 and Sp3 to connexin-response elements in osteoblast promoters

    NASA Technical Reports Server (NTRS)

    Stains, Joseph P.; Lecanda, Fernando; Screen, Joanne; Towler, Dwight A.; Civitelli, Roberto

    2003-01-01

    Loss-of-function mutations of gap junction proteins, connexins, represent a mechanism of disease in a variety of tissues. We have shown that recessive (gene deletion) or dominant (connexin45 overexpression) disruption of connexin43 function results in osteoblast dysfunction and abnormal expression of osteoblast genes, including down-regulation of osteocalcin transcription. To elucidate the molecular mechanisms of gap junction-sensitive transcriptional regulation, we systematically analyzed the rat osteocalcin promoter for sensitivity to gap junctional intercellular communication. We identified an Sp1/Sp3 containing complex that assembles on a minimal element in the -70 to -57 region of the osteocalcin promoter in a gap junction-dependent manner. This CT-rich connexin-response element is necessary and sufficient to confer gap junction sensitivity to the osteocalcin proximal promoter. Repression of osteocalcin transcription occurs as a result of displacement of the stimulatory Sp1 by the inhibitory Sp3 on the promoter when gap junctional communication is perturbed. Modulation of Sp1/Sp3 recruitment also occurs on the collagen Ialpha1 promoter and translates into gap junction-sensitive transcriptional control of collagen Ialpha1 gene expression. Thus, regulation of Sp1/Sp3 recruitment to the promoter may represent a potential general mechanism for transcriptional control of target genes by signals passing through gap junctions.

  17. Babesia bicornis sp. nov. and Theileria bicornis sp. nov.: tick-borne parasites associated with mortality in the black rhinoceros (Diceros bicornis).

    PubMed

    Nijhof, Ard M; Penzhorn, Banie L; Lynen, Godelieve; Mollel, Johnson O; Morkel, Pete; Bekker, Cornelis P J; Jongejan, Frans

    2003-05-01

    A novel Babesia species, designated Babesia bicornis sp. nov., was identified in three black rhinoceroses (Diceros bicornis) that died in wildlife areas in Tanzania and South Africa. Screening of black rhinoceroses in South Africa revealed, in addition to B. bicornis, a second parasite, designated Theileria bicornis sp. nov. PMID:12734294

  18. Geographic distribution of Theileria sp. (buffalo) and Theileria sp. (bougasvlei) in Cape buffalo (Syncerus caffer) in southern Africa: implications for speciation.

    PubMed

    Pienaar, Ronel; Latif, Abdalla A; Thekisoe, Oriel M M; Mans, Ben J

    2014-03-01

    Strict control measures apply to movement of buffalo in South Africa including testing for Theileria parva, the causative agent of Corridor disease in cattle. The official test is a real-time hybridization PCR assay that amplifies the 18S rRNA V4 hyper-variable region of T. parva, T. sp. (buffalo) and T. sp. (bougasvlei). Mixed infections with the latter organisms affect diagnostic sensitivity due to PCR suppression. While the incidence of mixed infections in the Corridor disease endemic region of South Africa is significant, little information is available on the specific distribution and prevalence of T. sp. (buffalo) and T. sp. (bougasvlei). Specific real-time PCR assays were developed and a total of 1211 samples known to harbour these parasites were screened. Both parasites are widely distributed in southern Africa and the incidence of mixed infections with T. parva within the endemic region is similar (?25-50%). However, a significant discrepancy exists in regard to mixed infections of T. sp. (buffalo) and T. sp. (bougasvlei) (?10%). Evidence for speciation between T. sp. (buffalo) and T. sp. (bougasvlei) is supported by phylogenetic analysis of the COI gene, and their designation as different species. This suggests mutual exclusion of parasites and the possibility of hybrid sterility in cases of mixed infections. PMID:24229841

  19. Heat Curing of a Sym Plasmid in a Fast-Growing Rhizobium sp. That Is Able to Nodulate Legumes and the Nonlegume Parasponia sp

    PubMed Central

    Morrison, Nigel A.; Hau, Cen Y.; Trinick, Michael J.; Shine, John; Rolfe, Barry G.

    1983-01-01

    Genes involved in nodulation of both legumes and the nonlegume Parasponia sp., as well as nitrogenase genes, reside on a large plasmid in a fast-growing Rhizobium sp. from Lablab purpureus. This plasmid can be cured by incubation at elevated temperatures and can be mobilized by the P1 group plasmid RP1::Tn501. Images PMID:6571729

  20. UNM online course enrollments by college: 2003-2005 SU 03 FA 03 SP 04 SU 04 FA 04 SP 05 SU 05 FA 05

    E-print Network

    UNM UNM online course enrollments by college: 2003-2005 SU 03 FA 03 SP 04 SU 04 FA 04 SP 05 SU 05) for academic courses. First online program leading towards a Bachelor's degree at UNM. The College of Nursing offers the R.N. to B.S.N. Completion Program. Academic Year 2001-2002: 17 online courses offered with 220

  1. Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163

    PubMed Central

    Dashti, Yousef; Grkovic, Tanja; Abdelmohsen, Usama Ramadan; Hentschel, Ute; Quinn, Ronald J.

    2014-01-01

    Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by 1H NMR. Ten known compounds, including angucycline, diketopiperazine and ?-carboline derivatives 1–10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes. PMID:24857962

  2. Influence of inoculation technique on the endophytic colonization of rice by Pantoea sp. isolated from sweet potato and by Enterobacter sp. isolated from sugarcane

    Microsoft Academic Search

    Muhammad Zakria; Kana Udonishi; Tsugufumi Ogawa; Akihiro Yamamoto; Yuichi Saeki; Shoichiro Akao

    2008-01-01

    The colonization abilities of two endophytic diazotrophs, Pantoea sp. strain 18 isolated from sweet potato and Enterobacter sp. strain 35 isolated from sugarcane, in cultivated rice (Oryza sativa cv. Nipponbare) and wild rice (Oryza officinalis) were investigated using two inoculation methods: rhizosphere inoculation and the root dip method. Higher bacterial densities resulted from the root dip method for both strains.

  3. Bioactive metabolites produced by Penicillium sp. 1 and sp. 2, two endophytes associated with Alibertia macrophylla (Rubiaceae).

    PubMed

    Oliveira, Camila M; Silva, Geraldo H; Regasini, Luis O; Zanardi, Lisinéia M; Evangelista, Alana H; Young, Maria C M; Bolzani, Vanderlan S; Araujo, Angela R

    2009-01-01

    In the course of our continuous search for bioactive metabolites from endophytic fungi living in plants from the Brazilian flora, leaves of Alibertia macrophylla (Rubiaceae) were submitted to isolation of endophytes, and two species of Penicillium were isolated. The acetonitrile fraction obtained in corn from a culture of Penicillium sp. 1 afforded orcinol (1). On the other hand, Penicillium sp. 1 cultivated in potato-dextrose-broth furnished two different compounds, cyclo-(L-Pro-L-Val) (2) and uracil (3). The chromatographic fractionation of the acetonitrile fraction obtained from Penicillium sp. 2 led to three dihydroisocoumarins, 4-hydroxymellein (4), 8-methoxymellein (5) and 5-hydroxymellein (6). Compounds 5 and 6 were obtained from the Penicillium genus for the first time. Additionally, metabolites 1-6 were evaluated for their antifungal and acetylcholinesterase (AChE) inhibitory activities. The most active compounds 1 and 4 exhibited detection limits of 5.00 and 10.0 microg against Cladosporium cladosporioides and C. sphaerospermum, respectively. Compound 2 showed a detection limit of 10.0 microg, displaying potent AChE inhibitory activity. PMID:20158153

  4. $SO/Sp$ Chern-Simons Gauge Theories At Large $N$, $SO/Sp$ Penner Models And The Gauge Group Volumes

    E-print Network

    Noureddine Chair; Mohammad Dalabeeh

    2012-06-07

    We construct a deformed $SO/Sp$ Penner generating function responsible for the close connection between $SO/Sp$ Chern-Simons gauge theories at large $N$ and the $SO/Sp$ Penner models. This construction is then shown to follow from a sector of a Chern-Simons gauge theory with coupling constant $\\lambda$. The free energy and its continuum limit of the perturbative Chern-Simons gauge theory are obtained from the Penner model. Finally, asymptotic expansions for the logarithm of the gauge group volumes are given for every genus $g\\geq 0$ and shown to be equivalent to the continuum limits of the $SO/Sp$ Chern-Simons gauge theories and the $SO/Sp$ Penner models

  5. Purification of native surfactant protein SP-A from pooled amniotic fluid and bronchoalveolar lavage.

    PubMed

    Karbani, Najmunisa; Dodagatta-Marri, Eswari; Qaseem, Asif S; Madhukaran, Priyaa; Waters, Patrick; Tsolaki, Anthony G; Madan, Taruna; Kishore, Uday

    2014-01-01

    Surfactant protein SP-A is a hydrophilic glycoprotein, similar to SP-D, which plays an important role in pulmonary surfactant homeostasis and innate immunity. SP-A is actively expressed in the alveolar type II cells and Clara cells. Their basic structure consists of triple-helical collagen region and a C-terminal carbohydrate recognition domain (CRD). By binding to the infectious microbes, SP-A (like SP-D) are involved in pathogen opsonization and agglutination and subsequent clearance of the microorganism, via recruitment of phagocytic cells via receptors for the collagen region. SP-A has also been localized at extra-pulmonary sites such as salivary epithelium, amniotic fluid, prostate glands, and semen. The presence of SP-A in fetal and maternal tissue and amniotic fluid suggests it is involved in pregnancy and labor. Native SP-A can be purified from amniotic fluid and bronchiolar lavage fluid (BALF) via affinity chromatography. In addition, we also report here a procedure to express and purify a recombinant form of trimeric CRD in Escherichia coli. The availability of highly pure native SP-A and CRD region can be central to studies that examine the diverse roles that SP-A play in surfactant homeostasis, pulmonary infection and inflammation and pregnancy. PMID:24218266

  6. In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement

    PubMed Central

    2011-01-01

    Background Mice lacking surfactant protein-A (SP-A-/-; knockout; KO) exhibit increased vulnerability to infection and injury. Although many bronchoalveolar lavage (BAL) protein differences between KO and wild-type (WT) are rapidly reversed in KO after infection, their clinical course is still compromised. We studied the impact of SP-A on the alveolar macrophage (AM) proteome under basal conditions. Male SP-A KO mice were SP-A-treated (5 micrograms/mouse) and sacrificed in 6 or 18 hr. The AM proteomes of KO, SP-A-treated KO, and WT mice were studied by 2D-DIGE coupled with MALDI-ToF/ToF and AM actin distribution was examined by phalloidon staining. Results We observed: a) significant differences from KO in WT or exogenous SP-A-treated in 45 of 76 identified proteins (both increases and decreases). These included actin-related/cytoskeletal proteins (involved in motility, phagocytosis, endocytosis), proteins of intracellular signaling, cell differentiation/regulation, regulation of inflammation, protease/chaperone function, and proteins related to Nrf2-mediated oxidative stress response pathway; b) SP-A-induced changes causing the AM proteome of the KO to resemble that of WT; and c) that SP-A treatment altered cell size and F-actin distribution. Conclusions These differences are likely to enhance AM function. The observations show for the first time that acute in vivo SP-A treatment of KO mice, under basal or unstimulated conditions, affects the expression of multiple AM proteins, alters F-actin distribution, and can restore much of the WT phenotype. We postulate that the SP-A-mediated expression profile of the AM places it in a state of "readiness" to successfully conduct its innate immune functions and ensure lung health. PMID:22035134

  7. Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir.

    PubMed

    Li, Ying; Kawamura, Yoshiaki; Fujiwara, Nagatoshi; Naka, Takashi; Liu, Hongsheng; Huang, Xinxiang; Kobayashi, Kazuo; Ezaki, Takayuki

    2004-05-01

    Four Gram-positive bacteria, strains A1-17B(T), A1-22(T), A1-3(T) and A1-8, isolated from the air in the Russian space laboratory Mir, were subjected to a polyphasic taxonomic study. Phylogenetic analysis of the bacteria based on their 16S rDNA sequence showed that they belong to the genera Rothia (A1-17B(T)), Rhodococcus (A1-22(T)) and Arthrobacter (A1-3(T) and A1-8). Morphological, physiological, chemotaxonomic and genomic characteristics supported the assignments of these strains to these genera, but they could not be classified as any existing species within each respective genus. 16S rDNA similarity values between strain A1-17B(T) and its neighbours, Rothia dentocariosa genomovar II, Rothia dentocariosa, Rothia mucilaginosa and Rothia nasimurium, were respectively 99.8, 98.0, 96.4 and 95.4 %. Polyphasic taxonomic evidence indicated that strain A1-17B(T) should be categorized together with the unofficially named Rothia dentocariosa genomovar II, but clearly differentiated them from the established species of the genus ROTHIA: Strain A1-22(T) formed a coherent cluster with Rhodococcus erythropolis, Rhodococcus globerulus, Rhodococcus marinonascens and Rhodococcus percolatus in 16S rDNA sequence analysis, but DNA-DNA relatedness values were only 45.5, 35.3, 18.9 and 21.9 %. Strains A1-3(T) and A1-8 shared 99.9 % 16S rDNA sequence similarity, and strain A1-3(T) showed the highest level of 16S rDNA similarity, 96.6 %, to Arthrobacter polychromogenes. Contrasting biochemical characteristics were also identified. Finally, as a result of the polyphasic taxonomic study, three of the strains are proposed as type strains of novel species: Rothia aeria sp. nov. (A1-17B(T)=GTC 867(T)=JCM 11412(T)=DSM 14556(T)), Rhodococcus baikonurensis sp. nov. (A1-22(T)=GTC 1041(T)=JCM 11411(T)=DSM 44587(T)) and Arthrobacter russicus sp. nov. (A1-3(T)=GTC 863(T)=JCM 11414(T)=DSM 14555(T)). PMID:15143031

  8. Demequina globuliformis sp. nov., Demequina oxidasica sp. nov. and Demequina aurantiaca sp. nov., actinobacteria isolated from marine environments, and proposal of Demequinaceae fam. nov.

    PubMed

    Ue, Harumi; Matsuo, Yoshihide; Kasai, Hiroaki; Yokota, Akira

    2011-06-01

    Three Gram-stain-positive actinobacterial strains, designated YM24-125(T), YM05-1041(T) and YM12-102(T), were isolated from marine environments. Phylogenetic analysis based on 16S rRNA gene sequences indicated that they belonged to the suborder Micrococcineae and were most closely related to members of the genus Demequina with 95.0-98.4 % sequence similarities. Cells of strains YM05-1041(T) and YM12-102(T) were rod-shaped, similar to members of the genus Demequina, while cells of strain YM24-125(T) were cocci to short ovoid rods. All strains grew in the absence of NaCl, but tolerated up to 15 % NaCl. The major menaquinone was demethylmenaquinone DMK-9 (H(4)) and the major polar lipids were phosphatidylinositol, diphosphatidylglycerol and phosphatidyglycerol. All strains contained straight-chain, iso-branched and anteiso-branched saturated fatty acids. The DNA G+C contents were 65.3-67.3 mol%. The peptidoglycans of strains YM05-1041(T) and YM12-102(T) were the A4? type with ornithine while that of strain YM24-125(T) contained ornithine and lysine as diamino acids. Phylogenetic analyses and differences in physiological and biochemical characteristics indicated that these three isolates represent novel species within the genus Demequina, for which the names Demequina globuliformis sp. nov. (with YM24-125(T) ?=?NBRC 106266(T) ?=?KCTC 19747(T) ?=?MBIC 08349(T) as the type strain), Demequina oxidasica sp. nov. (with YM05-1041(T) ?=?NBRC 106264(T) ?=?KCTC 19746(T) ?=?MBIC 08346(T) as the type strain) and Demequina aurantiaca sp. nov. (with YM12-102(T) ?=?NBRC 106265(T) ?=?KCTC 19745(T) ?=?MBIC 08347(T) as the type strain) are proposed. Based on differences in the quinone system, the presence of demethylmenaquinone DMK-9(H(4)) and the pattern of 16S rRNA signatures, the three novel strains and current members of the genus Demequina represent a unique family within the suborder Micrococcineae. Therefore, the family Demequinaceae fam. nov. is also proposed. PMID:20656823

  9. Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov.

    PubMed

    Gardan, Louis; Gouy, Cécile; Christen, Richard; Samson, Régine

    2003-03-01

    A collection of 42 strains belonging to the five subspecies of Pectobacterium carotovorum (subspecies atrosepticum, betavasculorum, carotovorum, odoriferum and wasabiae) and 11 reference and type strains of biovars of Pectobacterium chrysanthemi, Pectobacterium cacticidum and Brenneria paradisiaca were studied by DNA-DNA hybridization, numerical taxonomy of 120 phenotypic characteristics, serology and new phylogenetic analysis of previously reported sequences from a database of aligned 16S rDNA sequences. The P. carotovorum subspecies formed a clade according to neighbour-joining methods, but they formed two paraphyletic clusters according to maximum-likelihood and maximum-parsimony. However, phylogenetic analysis of 16S rDNA sequences alone is not sufficient to justify generic differentiation and therefore, it is proposed to retain the P. carotovorum subspecies in the genus Pectobacterium. The strains of P. carotovorum were distributed in four genomospecies: genomospecies 1, harbouring all strains of subsp. atrosepticum, genomospecies 2, including the strains of subsp. betavasculorum isolated from sugar beet, sunflower, potato, hyacinth and artichoke, genomospecies 3, clustering all strains of subsp. wasabiae isolated from wasabi in Japan, and genomospecies 4, gathering together strains of subsp. carotovorum and strains of subsp. odoriferum. Four strains of P. carotovorum subsp. carotovorum remained unclustered. Biochemical criteria, deduced from a numerical taxonomy study of phenotypic characteristics and serological reactions, allowed discrimination of strains belonging to the four genomospecies. Thus, it is proposed that three genomospecies be elevated to species level as Pectobacterium atrosepticum sp. nov. (type strain CFBP 1526T =LMG 2386T =NCPPB 549T =ICMP 1526T), Pectobacterium betavasculorum sp. nov. (type strain CFBP 2122T =LMG 2464T =NCPPB 2795T =ICMP 4226T) and Pectobacterium wasabiae sp. nov. (type strain CFBP 3304T =LMG 8404T =NCPPB 3701T =ICMP 9121T). Only two subspecies are maintained within P. carotovorum, subsp. carotovorum (type strain CFBP 2046T =LMG 2404T =NCPPB 312T =ICMP 5702T) and subsp. odoriferum (type strain CFBP 1878T =LMG 5863T =NCPPB 3839T = ICMP 11553T), for which discriminating tests are available. PMID:12710602

  10. Palladium-catalyzed unactivated ?-methylene C(sp(3))-H bond alkenylation of aliphatic amides and its application in a sequential C(sp(3))-H/C(sp(2))-H bond alkenylation.

    PubMed

    Shan, Gang; Huang, Guiyi; Rao, Yu

    2015-01-21

    A palladium(II)-catalyzed ?-methylene C(sp(3))-H bond alkenylation of acyclic aliphatic amides with alkenyl halides has been developed. Both (E)-olefins and (Z)-olefins can be readily accessed using this method and a possible (Z)/(E)-olefin isomerization pathway is proposed. A solvent effect-promoted sequential C(sp(3))-H bond alkenylation and C(sp(2))-H bond alkenylation was also studied, and can provide a convenient route to novel diene compounds. PMID:25429854

  11. Low prevalence of Blastocystis sp. in active ulcerative colitis patients.

    PubMed

    Rossen, N G; Bart, A; Verhaar, N; van Nood, E; Kootte, R; de Groot, P F; D'Haens, G R; Ponsioen, C Y; van Gool, T

    2015-05-01

    Ulcerative colitis (UC) is thought to originate from a disbalance in the interplay between the gut microbiota and the innate and adaptive immune system. Apart from the bacterial microbiota, there might be other organisms, such as parasites or viruses, that could play a role in the aetiology of UC. The primary objective of this study was to compare the prevalence of Blastocystis sp. in a cohort of patients with active UC and compare that to the prevalence in healthy controls. We studied patients with active UC confirmed by endoscopy included in a randomised prospective trial on the faecal transplantation for UC. A cohort of healthy subjects who served as donors in randomised trials on faecal transplantation were controls. Healthy subjects did not have gastrointestinal symptoms and were extensively screened for infectious diseases by a screenings questionnaire, extensive serologic assessment for viruses and stool analysis. Potential parasitic infections such as Blastocystis were diagnosed with the triple faeces test (TFT). The prevalence of Blastocystis sp. were compared between groups by Chi-square testing. A total of 168 subjects were included, of whom 45 had active UC [median age 39.0 years, interquartile range (IQR) 32.5-49.0, 49 % male] and 123 were healthy subjects (median age 27 years, IQR 22.0-37.0, 54 % male). Blastocystis sp. was present in the faeces of 40/123 (32.5 %) healthy subjects and 6/45 (13.3 %) UC patients (p?=?0.014). Infection with Blastocystis is significantly less frequent in UC patients as compared to healthy controls. PMID:25680316

  12. Effect of salt stress on the physiology of Frankia sp strain CcI6.

    PubMed

    Oshone, Rediet; Mansour, Samira R; Tisa, Louis S

    2013-11-01

    Actinorhizal plants are able to overcome saline soils and reclaim land. Frankia sp strain CcI6 was isolated from nodules of Casuarina cunninghamiana found in Egypt. Phylogenetic analysis of Frankia sp. strain CcI6 revealed that the strain is closely related to Frankia sp. strain CcI3. The strain displays an elevated level of NaCl tolerance. Vesicle production and nitrogenase activity were also influenced by NaCl. PMID:24287648

  13. Human Sug1/p45 is involved in the proteasome-dependent degradation of Sp1.

    PubMed Central

    Su, K; Yang, X; Roos, M D; Paterson, A J; Kudlow, J E

    2000-01-01

    The transcription factor Sp1 was previously shown to undergo proteasome-dependent degradation when cells were glucose-starved and stimulated with the adenylate cyclase inducer, forskolin. However, the control of the Sp1 degradation process is largely unknown. Using in vitro and in vivo interaction studies, we show in the present study that Sp1 interacts with human Sug1 [hSug1, also known as p45 or thyroid-hormone-receptor interacting protein ('TRIP1')], an ATPase subunit of the 26 S proteasome and a putative transcriptional modulator. This interaction with Sp1 occurs through the C-terminus of hSug1, the region that contains the conserved ATPase domain in this protein. Both in vitro studies, in reconstituted degradation assays, and in vivo experiments, in which hSug1 is overexpressed in normal rat kidney cells, show that full-length hSug1 is able to stimulate the proteasome-dependent degradation of Sp1. However, hSug1 truncations that lack either the N- or C-terminal domain of hSug1 act as dominant negatives, inhibiting Sp1 degradation in vitro. Also, an ATPase mutant of hSug1, while still able to bind Sp1, acts as a dominant negative, blocking Sp1 degradation both in vitro and in vivo. These results demonstrate that hSug1 is involved in the degradation of Sp1 and that ATP hydrolysis by hSug1 is necessary for this process. Our findings indicate that hSug1 is an exchangeable proteasomal component that plays a critical regulatory role in the proteasome-dependent degradation of Sp1. However, hSug1 is not the factor limiting Sp1 degradation in the cells treated with glucosamine. This and other considerations suggest that hSug1 co-operation with other molecules is necessary to target Sp1 for proteasome degradation. PMID:10816420

  14. Sp1, a new biomarker that identifies a subset of aggressive pancreatic ductal adenocarcinoma.

    PubMed

    Jiang, Naomi Y; Woda, Bruce A; Banner, Barbara F; Whalen, Giles F; Dresser, Karen A; Lu, Di

    2008-07-01

    Pancreatic adenocarcinoma is one of the leading causes of cancer-related deaths in the United States. Sp1 is a sequence-specific DNA binding protein that is important in the transcription of a number of regulatory genes involved in cancer cell growth, differentiation, and metastasis. In this study, we investigated Sp1 expression in pancreatic ductal adenocarcinoma and its association with clinical outcome. We studied 42 patients with primary pancreatic adenocarcinoma. The expression of Sp1 in pancreatic adenocarcinoma was evaluated by immunohistochemical staining. All 42 patients had clinical follow-up information and were evaluated for survival. Sp1 protein was aberrantly overexpressed in a subset of primary pancreatic adenocarcinoma. These tumors all developed metastasis, whereas none of the primary tumors without lymph node metastasis showed Sp1 overexpression. Statistically, Sp1 overexpression was associated with higher stage, higher grade, and lymph node metastasis (P < 0.001, P = 0.036, and P < 0.0001, respectively). Additionally, patients of this subset had a much shorter overall survival than patients without Sp1 overexpression, as evidenced by Kaplan-Meier plots and the log-rank test (P = 0.002). The 5-year overall survival rate was 19% in patients with Sp1 overexpression, compared with 55% in patients without Sp1 overexpression. The median survival was only 13 months for patients with Sp1 overexpression, compared with 65 months for patients without Sp1 overexpression. In conclusion, Sp1 is a new biomarker that identifies a subset of pancreatic ductal adenocarcinoma with aggressive clinical behavior. It can be used at initial diagnosis of pancreatic adenocarcinoma to identify patients with an increased probability of cancer metastasis and much shortened overall survival. PMID:18628415

  15. Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy

    Microsoft Academic Search

    Dionigia Meloni; Giovanna Sanciu; Philippe Poirier; Hicham El Alaoui; Magali Chabé; Laurence Delhaes; Eduardo Dei-Cas; Frederic Delbac; Pier Luigi Fiori; David Di Cave; Eric Viscogliosi

    Blastocystis sp. is the most common eukaryotic parasite in the intestinal tract of humans. Due to its potential impact in public health,\\u000a we determined the Blastocystis sp. subtypes (STs) and their relative frequency in symptomatic patients living in or in the vicinity of two Italian cities\\u000a (Rome and Sassari). A total of 34 Blastocystis sp. isolates corresponding to 26 single

  16. MPI-LAPI: An Efficient Implementation of MPI for IBM RS\\/6000 SP Systems

    Microsoft Academic Search

    Mohammad Banikazemi; Rama K. Govindaraju; Robert Blackmore; Dhabaleswar K. Panda

    2001-01-01

    The IBM RS\\/6000 SP system is one of the most cost-effective commercially available highperformance machines. IBM RS\\/6000 SP systems support the Message Passing Interfacestandard (MPI) and LAPI. LAPI is a low level, reliable and efficient one sided communicationAPI library, implemented on IBM RS\\/6000 SP systems. This paper explains howthe high performance of the LAPI library has been exploited in order

  17. A 3-D time-dependent unstructured tetrahedral-mesh SP{sub N} method

    SciTech Connect

    Morel, J.E.; McGhee, J.M. [Los Alamos National Lab., NM (United States); Larsen, E.W. [Michigan Univ., Ann Arbor, MI (United States). Dept. of Nuclear Engineering

    1994-10-01

    We have developed a 3-D time-dependent multigroup SP{sub n} method for unstructured tetrahedral meshes. The SP{sub n} equations are expressed in a canonical form which allows them to be solved using standard diffusion solution techniques in conjunction with source iteration, diffusion-synthetic acceleration, and fission-source acceleration. A computational comparison of our SP{sub n} method with an even-parity S{sub n} method is given.

  18. Bromopyrrole alkaloids from a marine sponge Agelas sp.

    PubMed

    Kusama, Taishi; Tanaka, Naonobu; Takahashi-Nakaguchi, Azusa; Gonoi, Tohru; Fromont, Jane; Kobayashi, Jun'ichi

    2014-01-01

    Five new bromopyrrole alkaloids, 2-bromokeramadine (1), 2-bromo-9,10-dihydrokeramadine (2), tauroacidins C (3) and D (4), and mukanadin G (5), were isolated from an Okinawan marine sponge Agelas sp. The structures of 1-5 were elucidated on the basis of spectroscopic data and conformational analysis. Mukanadin G (5) has a tricyclic skeleton consisting of a fused tetrahydrobenzaminoimidazole and 2,5-dioxopyrrolidine moieties. Antimicrobial activities of 1-3, and 5 as well as three related known bromopyrrole alkaloids, keramadine (6), tauroacidin A (7), and taurodispacamide A (8) were evaluated. PMID:24789935

  19. Singlet-assisted supersymmetry breaking for Sp(2N) theories

    NASA Astrophysics Data System (ADS)

    Zhu, Bin; Meng, Kun; Ding, Ran; Li, Qiyi

    2013-07-01

    We investigate local supersymmetry-breaking vacua in s-confining theories with gauge group Sp(2N). By adapting the general recipe developed by Shadmi and Shirman, we construct a realistic model based on dynamics of SQCD coupled with singlets which allows a spontaneously broken supersymmetry. Since the chiral superfields in model have R-charges R = 0 and R = 2 only, the tedious computations of Coleman-Weinberg potential can be greatly alleviated from the lesson of David Shih. We observe that the pseudomoduli fields are stabilized at the origin of moduli space at one-loop order with calculability being preserved.

  20. Genome sequence and description of Aeromicrobium massiliense sp. nov.

    PubMed Central

    Ramasamy, Dhamodharan; Kokcha, Sahare; Lagier, Jean-Christophe; Nguyen, Thi-Thien; Raoult, Didier

    2012-01-01

    Aeromicrobium massiliense strain JC14Tsp. nov. is the type strain of Aeromicrobium massiliense sp. nov., a new species within the genus Aeromicrobium. This strain, whose genome is described here, was isolated from the fecal microbiota of an asymptomatic patient. Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes. PMID:23408663

  1. New isolate of Streptomyces sp. with novel thermoalkalotolerant cellulases

    Microsoft Academic Search

    Faiez Alani; William A. Anderson; Murray Moo-Young

    2008-01-01

    A Streptomyces sp. was isolated that produced novel thermoalkalotolerant cellulase activity after growth on crystalline cellulose at 50?C.\\u000a Three major components of the cellulases (CMCase, Avicelase and cellobiase) were produced with maximal activities (11.8, 7.8\\u000a and 3.9 IU\\/ml) and maximum specific activities 357, 276 and 118 IU\\/mg protein, respectively, after 120 h growth. Maximum CMCase\\u000a activity was between 50 and 60?C measured over

  2. Polyketides from a Marine-Derived Fungus Xylariaceae sp.

    PubMed Central

    Nong, Xu-Hua; Zheng, Zhi-Hui; Zhang, Xiao-Yong; Lu, Xin-Hua; Qi, Shu-Hua

    2013-01-01

    Eighteen polyketides (1–18) including six citrinin derivatives, two phenol derivatives, one cyclopentenone, two naphthol derivatives, and seven tetralone derivatives were isolated from the culture broth of a marine-derived fungal strain Xylariaceae sp. SCSGAF0086. Five of these compounds (1, 2, 8, 9, and 10) were new, and their structures were determined by spectroscopic methods. Compounds 4, 6, 7, and 17 showed enzyme-inhibitory activities towards several tested enzymes, and 6 and 7 showed strong antifouling activity against Bugula neritina larvae settlement. This is the first time that the antifouling and enzyme-inhibitory activities of these compounds has been reported. PMID:23697953

  3. Uranium biosorption by Padina sp. algae biomass: kinetics and thermodynamics

    Microsoft Academic Search

    Mohammad Hassan Khani

    Introduction  Kinetic, thermodynamic, and equilibrium isotherms of the biosorption of uranium ions onto Padina sp., a brown algae biomass, in a batch system have been studied.\\u000a \\u000a \\u000a \\u000a \\u000a Discussion  The kinetic data were found to follow the pseudo-second-order model. Intraparticle diffusion is not the sole rate-controlling\\u000a factor. The equilibrium experimental results were analyzed in terms of Langmuir isotherm depending with temperature. Equilibrium\\u000a data fitted

  4. Polyketides from a marine-derived fungus Xylariaceae sp.

    PubMed

    Nong, Xu-Hua; Zheng, Zhi-Hui; Zhang, Xiao-Yong; Lu, Xin-Hua; Qi, Shu-Hua

    2013-05-01

    Eighteen polyketides (1-18) including six citrinin derivatives, two phenol derivatives, one cyclopentenone, two naphthol derivatives, and seven tetralone derivatives were isolated from the culture broth of a marine-derived fungal strain Xylariaceae sp. SCSGAF0086. Five of these compounds (1, 2, 8, 9, and 10) were new, and their structures were determined by spectroscopic methods. Compounds 4, 6, 7, and 17 showed enzyme-inhibitory activities towards several tested enzymes, and 6 and 7 showed strong antifouling activity against Bugula neritina larvae settlement. This is the first time that the antifouling and enzyme-inhibitory activities of these compounds has been reported. PMID:23697953

  5. Amino Acid-Derived Metabolites from the Ascidian Aplidium sp.

    PubMed Central

    Won, Tae Hyung; Kim, Chang-Kwon; Lee, So-Hyoung; Rho, Boon Jo; Lee, Sang Kook; Oh, Dong-Chan; Oh, Ki-Bong; Shin, Jongheon

    2015-01-01

    Four new iodobenzene-containing dipeptides (1–4), a related bromotryptophan-containing dipeptide (5), and an iodophenethylamine (6) were isolated from the ascidian Aplidium sp. collected off the coast of Chuja-do, Korea. The structures of these novel compounds, designated as apliamides A–E (1–5) and apliamine A (6) were determined via combined spectroscopic analyses. The absolute configuration of the amino acid residue in 1 was determined by advanced Marfey’s analysis. Several of these compounds exhibited moderate cytotoxicity and significant inhibition against Na+/K+-ATPase (4). PMID:26087023

  6. Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803

    PubMed Central

    Selstam, Eva; Norling, Birgitta

    2015-01-01

    The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that “light” plasma membranes have a high carotenoid/protein ratio, when compared to “heavier” plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of ?-carotene precursor molecules and echinenone. PMID:26083372

  7. Seladonia (Pachyceble) henanensis sp. n. (Hymenoptera, Halictidae) from China

    PubMed Central

    Murao, Ryuki; Tadauchi, Osamu; Huan-li, Xu

    2013-01-01

    Abstract Seladonia (Pachyceble) henanensis sp. n., is described from Henan Province, the eastern-central part of China. This species is separated from its allied species by a combination of the following morphological characters: head broad in female, inner hind tibial spur of female with 7–8 slender teeth, T1 basolaterally with appressed hair tuft in both sexes, and genitalia with long and large lower gonostylus in male. Important taxonomic characters are illustrated with photographs, scanning electron micrographs, and line drawings. PMID:23794911

  8. Degradation of 2-Chloroallylalcohol by a Pseudomonas sp

    SciTech Connect

    Waarde, J.J. van der; Kok, R.; Janssen, D.B. (Univ. of Groningen (Netherlands))

    1993-02-01

    Halogenated aliphatic hydrocarbons are an important class of environmental pollutants. Biodegradation can be useful in clean-up of contaminated water and soil, provided efficient microorganisms are available. This study describes microbial growth on 2-chloroallylalcohol and proposes a degradation pathway. Bacteria cultures able to grow on this substrate were isolated and identified as Pseudomonas sp. Degradation of the chemical (via 2-chloroacrylic acid) was accompanied by dechlorination, indicating detoxication. The results suggest that the catabolic pathway of chloroallyalcohol and its dechlorination are specific for this chlorinated compound.

  9. Kalicephalus sp. in a captive Russell's viper: a case report.

    PubMed

    Kavitha, K T; Latha, B R; Bino Sundar, S T; Jayathangaraj, M G; Senthil Kumar, K; Sridhar, R; Abdul Basith, S

    2014-09-01

    The occurrence of Kalicephalus sp. of hookworms in a Russell's viper snake maintained in Chennai snake park trust, Chennai is reported. These worms were found in the stomach and intestine. They were cylindrical, thread like and had an elongate body. Anterior end was obliquely truncated with bivalvular buccal capsule. Short, thick and muscularised oesophagus ending in a rounded bulb was observed. In males, a well developed trilobed bursa was observed and the spicules were short and equal. Typical strongyle type eggs were found in the uterus of female worms as well as in the intestinal contents. PMID:25035588

  10. Arrhopalites potapovi sp. nov. (Collembola, Symphypleona) from Russia.

    PubMed

    Vargovitsh, Robert S

    2015-01-01

    A new springtail species of the family Arrhopalitidae, Arrhopalites potapovi sp. nov., is described. It belongs to the caecus species group characterized by 3,2,1,1,1 anterior setae on dens and separates from Arrhopalites caecus (Tullberg) and other congeners by strongly differentiated cuticular spines on sixth abdominal segment, shape of female subanal appendages and foot complex. It was found under the loose bark of trees from two localities along Lake Baikal and it is the second representative of the genus Arrhopalites Börner sensu stricto in Russia. PMID:25947839

  11. Novel Adociaquinone Derivatives from the Indonesian Sponge Xestospongia sp.

    PubMed Central

    He, Fei; Mai, Linh H.; Longeon, Arlette; Copp, Brent R.; Loaëc, Nadège; Bescond, Amandine; Meijer, Laurent; Bourguet-Kondracki, Marie-Lise

    2015-01-01

    Seven new adociaquinone derivatives, xestoadociaquinones A (1a), B (1b), 14-carboxy-xestoquinol sulfate (2) and xestoadociaminals A–D (3a, 3c, 4a, 4c), together with seven known compounds (5–11) were isolated from an Indonesian marine sponge Xestospongia sp. Their structures were elucidated by extensive 1D and 2D NMR and mass spectrometric data. All the compounds were evaluated for their potential inhibitory activity against eight different protein kinases involved in cell proliferation, cancer, diabetes and neurodegenerative disorders as well as for their antioxidant and antibacterial activities. PMID:25927661

  12. A new sesquiterpene from endophytic fungus Aspergillus sp

    Microsoft Academic Search

    Shao-Liu Luo; Guo-Hong Li; Fang-Fang Liu; Li-Ping Lei; Zhen-Yuan Xia; Ke-Qin Zhang

    2011-01-01

    A fungal strain named YLF-14 was isolated from the leaf of Altingia yunnunensis. Based on the sequence at the internal transcribed spacer (ITS) region, the strain was identified as an Aspergillus sp.. A new sesquiterpene 5?,8a?-dimethyl-3,4,4a?,5,6,7,8,8a-octahydronaphthalene-1,2,5?-trimethanol (1) and a known compound 12-N-methylcyclo-(L-tryptophyl-L-phenylalanyl) (2) were isolated from the culture of this strain. Bioassay experiments showed that the two compounds had no

  13. A new sesquiterpene from endophytic fungus Aspergillus sp

    Microsoft Academic Search

    Shao-Liu Luo; Guo-Hong Li; Fang-Fang Liu; Li-Ping Lei; Zhen-Yuan Xia; Ke-Qin Zhang

    2012-01-01

    A fungal strain named YLF-14 was isolated from the leaf of Altingia yunnunensis. Based on the sequence at the internal transcribed spacer (ITS) region, the strain was identified as an Aspergillus sp.. A new sesquiterpene 5?,8a?-dimethyl-3,4,4a?,5,6,7,8,8a-octahydronaphthalene-1,2,5?-trimethanol (1) and a known compound 12-N-methylcyclo-(L-tryptophyl-L-phenylalanyl) (2) were isolated from the culture of this strain. Bioassay experiments showed that the two compounds had no

  14. Vitamin requirements of Fusarium oxysporum f. sp. vasinfectum.

    PubMed

    El-Abyad, M S; Ramadan, Z M

    1979-01-01

    The effect of eight water-soluble vitamins on germination, germ-tube extension, growth, and sporulation of Fusarium oxysporum Schl. f.sp. vasinfectum (Atk.) Snyder and Hansen, was studied. Each vitamin was utilized in eight different concentrations. The fungus responded favourably to all of the utilized vitamins in almost all the concentrations where germination, growth, and sporulation were substantially greater than the controls. Among the vitamins used, the fungus appeared to be highly sensitive to thiamine and pyridoxine, moderately sensitive to inositol and pantothenate, and least affected by folic acid. PMID:543346

  15. Synthesis of highly crystalline sp2-bonded boron nitride aerogels.

    PubMed

    Rousseas, Michael; Goldstein, Anna P; Mickelson, William; Worsley, Marcus A; Woo, Leta; Zettl, Alex

    2013-10-22

    sp(2)-Bonded boron nitride aerogels are synthesized from graphene aerogels via carbothermal reduction of boron oxide and simultaneous nitridation. The color and chemical composition of the original gel change dramatically, while structural features down to the nanometer scale are maintained, suggesting a direct conversion of the carbon lattice to boron nitride. Scanning and transmission electron microscopies reveal a foliated architecture of wrinkled sheets, a unique morphology among low-density, porous BN materials. The converted gels display a high degree of chemical purity (>95%) and crystalline order and exhibit unique cross-linking structures. PMID:24011289

  16. Nonomuraea terrinata sp. nov., a novel soil actinomycete.

    PubMed

    Quintana, Erika; Maldonado, Luis; Goodfellow, Michael

    2003-01-01

    The taxonomic position of a soil isolate, strain E626, was evaluated using the polyphasic approach. The organism was found to have chemical and morphological features consistent with its assignment to the genus Nonomuraea, a member of the family Streptosporangiaceae. Strain E626 consistently formed a distinct phyletic line within the Streptosporangiaceae 16S rDNA tree using four different algorithms. Furthermore, the taxonomic distinctness of the organism is underpinned by a range of phenotypic properties, notably morphological features. It is, therefore, proposed that the organism be classified in the genus Nonomuraea as Nonomuraea terrinata sp. nov. PMID:12906356

  17. New megastigmane glycoside and alkaloids from Streptomyces sp. YIM 63342.

    PubMed

    Yang, Xue-Qiong; Yang, Ya-Bin; Zhou, Hao; He, Guang-Wei; Zhao, Li-Xing; Xu, Li-Hua; Ding, Zhong-Tao

    2013-01-01

    New sesquiterpene glycoside, cyclodipeptide and piperidine derivative were isolated from Streptomyces sp. YIM 63342. On the basis of spectral data, their structures were determined as 3R, 5R, 6S, 7E, 9R-megastigman-7-en-3,5,6,9-tetrol-9-O-?-D-apiofuranosyl-(1??2)-?-D-glucopyranoside (1), cyclo (L-Pro-L-OMet) (2) and (R)-(E, E)-2-(l,3-pentadienyl) piperidine (3), together with three known compounds as N-acetyltyramine (4), lycoperodine-1 (5), cyclo(L-Pro-L-Tyr)(6). PMID:22934740

  18. Cupriavidus plantarum sp. nov., a plant-associated species.

    PubMed

    Estrada-de Los Santos, Paulina; Solano-Rodríguez, Roosivelt; Matsumura-Paz, Lucía Tomiko; Vásquez-Murrieta, María Soledad; Martínez-Aguilar, Lourdes

    2014-11-01

    During a survey of plant-associated bacteria in northeast Mexico, a group of 13 bacteria was isolated from agave, maize and sorghum plants rhizosphere. This group of strains was related to Cupriavidus respiraculi (99.4 %), but a polyphasic investigation based on DNA-DNA hybridization analysis, other genotypic studies and phenotypic features showed that this group of strains actually belongs to a new Cupriavidus species. Consequently, taking all the results together, the description of Cupriavidus plantarum sp. nov. is proposed. PMID:25098225

  19. [Biological activity of Penicillium sp. 10-51 exometabolites].

    PubMed

    Savchuk, Ia I; Za?chenko, A M; Tsyganenko, E S

    2012-01-01

    Silica gel column chromatography (silica gel "L" II kind of activity 100/160 mkm) of the chloroform extract from the cultural filtrate of Penicillium sp. 10-51 gave two fractions (chloroform and chloroform-acetone, 5:1) having biological activity. Recrystallization yielded two compounds. On the basis of physico-chemical and spectral data these compounds were identified as curvularin and hydroxycurvularin, which have a large spectrum of biological action as to bacteria, yeast, blue-green algae and phytopathogenic micromycetes. PMID:23088100

  20. A novel phytotoxic nonenolide from Phomopsis sp. HCCB03520.

    PubMed

    Yang, Zhijun; Ge, Mei; Yin, Yu; Chen, Yi; Luo, Minyu; Chen, Daijie

    2012-02-01

    A novel phytotoxic nonenolide, (6S,7R,9R)-6,7-dihydroxy-9-propylnon-4-eno-9-lactone (1), was isolated from solid cultures of the endophytic fungus Phomopsis sp. HCCB03520, together with three known compounds, cytochalasin H (2), cytochalasin N (3), and epoxycytochalasin H (4). The structures of these compounds were elucidated through spectroscopic analysis, and the absolute configurations were determined by CD spectroscopy. Phytotoxic activities of compounds 1-4 were also investigated. Compound 1 showed phytotoxic activity on germination and radicle growth of Medicago sativa, Trifolium hybridum, and Buchloe dactyloides. PMID:22344916

  1. Novel Adociaquinone Derivatives from the Indonesian Sponge Xestospongia sp.

    PubMed

    He, Fei; Mai, Linh H; Longeon, Arlette; Copp, Brent R; Loaëc, Nadège; Bescond, Amandine; Meijer, Laurent; Bourguet-Kondracki, Marie-Lise

    2015-01-01

    Seven new adociaquinone derivatives, xestoadociaquinones A (1a), B (1b), 14-carboxy-xestoquinol sulfate (2) and xestoadociaminals A-D (3a, 3c, 4a, 4c), together with seven known compounds (5-11) were isolated from an Indonesian marine sponge Xestospongia sp. Their structures were elucidated by extensive 1D and 2D NMR and mass spectrometric data. All the compounds were evaluated for their potential inhibitory activity against eight different protein kinases involved in cell proliferation, cancer, diabetes and neurodegenerative disorders as well as for their antioxidant and antibacterial activities. PMID:25927661

  2. External reflection absorption infrared spectroscopy study of lung surfactant proteins SP-B and SP-C in phospholipid monolayers at the air/water interface.

    PubMed Central

    Pastrana-Rios, B; Taneva, S; Keough, K M; Mautone, A J; Mendelsohn, R

    1995-01-01

    The interactions of the hydrophobic pulmonary surfactant proteins SP-B and SP-C with 1,2-dipalmitoylphosphatidylcholine in mixed, spread monolayer films have been studied in situ at the air/water interface with the technique of external reflection absorption infrared spectroscopy (IRRAS). SP-C has a mostly alpha-helical secondary structure both in the pure state and in the presence of lipids, whereas SP-B secondary structure is a mixture of alpha-helical and disordered forms. When films of SP-B/1,2-dipalmitoylphosphatidylcholine are compressed to surface pressures (pi) greater than approximately 40-43 mN/m, the protein is partially (15-35%) excluded from the surface, as measured by intensity ratios of the peptide bond amide l/lipid C==O stretching vibrations. The extent of exclusion increases as the protein/lipid ratio in the film increases. In contrast, SP-C either remains at the surface at high pressures or leaves accompanied by lipids. The amide l peak of SP-C becomes asymmetric as a result of the formation of intermolecular sheet structures (1615-1630 cm-1) suggestive of peptide aggregation. The power of the IRRAS experiment for determination of film composition and molecular structure, i.e., as a direct test of the squeeze-out hypothesis of pulmonary surfactant function, is evident from this work. PMID:8599660

  3. Potential Role for CA-SP in Nucleating Retroviral Capsid Maturation

    PubMed Central

    England, Matthew R.; Purdy, John G.; Ropson, Ira J.; Dalessio, Paula M.

    2014-01-01

    ABSTRACT During virion maturation, the Rous sarcoma virus (RSV) capsid protein is cleaved from the Gag protein as the proteolytic intermediate CA-SP. Further trimming at two C-terminal sites removes the spacer peptide (SP), producing the mature capsid proteins CA and CA-S. Abundant genetic and structural evidence shows that the SP plays a critical role in stabilizing hexameric Gag interactions that form immature particles. Freeing of CA-SP from Gag breaks immature interfaces and initiates the formation of mature capsids. The transient persistence of CA-SP in maturing virions and the identification of second-site mutations in SP that restore infectivity to maturation-defective mutant viruses led us to hypothesize that SP may play an important role in promoting the assembly of mature capsids. This study presents a biophysical and biochemical characterization of CA-SP and its assembly behavior. Our results confirm cryo-electron microscopy (cryo-EM) structures reported previously by Keller et al. (J. Virol. 87:13655–13664, 2013, doi:10.1128/JVI.01408-13) showing that monomeric CA-SP is fully capable of assembling into capsid-like structures identical to those formed by CA. Furthermore, SP confers aggressive assembly kinetics, which is suggestive of higher-affinity CA-SP interactions than observed with either of the mature capsid proteins. This aggressive assembly is largely independent of the SP amino acid sequence, but the formation of well-ordered particles is sensitive to the presence of the N-terminal ?-hairpin. Additionally, CA-SP can nucleate the assembly of CA and CA-S. These results suggest a model in which CA-SP, once separated from the Gag lattice, can actively promote the interactions that form mature capsids and provide a nucleation point for mature capsid assembly. IMPORTANCE The spacer peptide is a documented target for antiretroviral therapy. This study examines the biochemical and biophysical properties of CA-SP, an intermediate form of the retrovirus capsid protein. The results demonstrate a previously unrecognized activity of SP in promoting capsid assembly during maturation. PMID:24719425

  4. Sp8 and COUP-TF1 reciprocally regulate patterning and Fgf signaling in cortical progenitors.

    PubMed

    Borello, Ugo; Madhavan, Mayur; Vilinsky, Ilya; Faedo, Andrea; Pierani, Alessandra; Rubenstein, John; Campbell, Kenneth

    2014-06-01

    To gain new insights into the transcriptional regulation of cortical development, we examined the role of the transcription factor Sp8, which is downstream of Fgf8 signaling and known to promote rostral cortical development. We have used a binary transgenic system to express Sp8 throughout the mouse telencephalon in a temporally restricted manner. Our results show that misexpression of Sp8 throughout the telencephalon, at early but not late embryonic stages, results in cortical hypoplasia, which is accompanied by increased cell death, reduced proliferation, and precocious neuronal differentiation. Misexpression of Sp8 at early developmental stages represses COUP-TF1 expression, a negative effector of Fgf signaling and a key promoter of posterior cortical identity, while ablation of Sp8 has the opposite effect. In addition, transgenic misexpression of COUP-TF1 resulted in downregulation of Sp8, indicating a reciprocal cross-regulation between these 2 transcription factors. Although Sp8 has been suggested to induce and/or maintain Fgf8 expression in the embryonic telencephalon, neither Fgf8 nor Fgf15 was upregulated using our gain-of-function approach. However, misexpression of Sp8 greatly increased the expression of Fgf target molecules, suggesting enhanced Fgf signaling. Thus, we propose that Sp8 promotes rostral and dorsomedial cortical development by repressing COUP-TF1 and promoting Fgf signaling in pallial progenitors. PMID:23307639

  5. Migration, site selection, and development of Ornithodiplostomum sp. metacercariae (Digenea: Strigeoidea) in fathead minnows (Pimephales promelas)

    E-print Network

    Wisenden, Brian D.

    : Strigeoidea) in fathead minnows (Pimephales promelas) Chelsea E. Matisz *, Cameron P. Goater Department of Ornithodiplostomum sp. metacercariae in their second intermediate host, the fathead minnow, Pimephales promelas

  6. Whole genome analyses of marine fish pathogenic isolate, Mycobacterium sp. 012931.

    PubMed

    Kurokawa, Satoru; Kabayama, Jun; Hwang, Seong Don; Nho, Seong Won; Hikima, Jun-ichi; Jung, Tae Sung; Kondo, Hidehiro; Hirono, Ikuo; Takeyama, Haruko; Mori, Tetsushi; Aoki, Takashi

    2014-10-01

    Mycobacterium is a genus within the order Actinomycetales that comprises of a large number of well-characterized species, several of which includes pathogens known to cause serious disease in human and animal. Here, we report the whole genome sequence of Mycobacterium sp. strain 012931 isolated from the marine fish, yellowtail (Seriola quinqueradiata). Mycobacterium sp. 012931 is a fish pathogen causing serious damage to aquaculture farms in Japan. DNA dot plot analysis showed that Mycobacterium sp. 012931 was more closely related to Mycobacterium marinum when compared across several Mycobacterium species. However, little conservation of the gene order was observed between Mycobacterium sp. 012931 and M. marinum genome. The annotated 5,464 genes of Mycobacterium sp. 012931 was classified into 26 subsystems. The insertion/deletion gene analysis shows Mycobacterium sp. 012931 had 643 unique genes that were not found in the M. marinum strains. In the virulence, disease, and defense subsystem, both insertion and deletion genes of Mycobacterium sp. 012931 were associated with the PPE gene cluster of Mycobacteria. Of seven plcB genes in Mycobacterium sp. 012931, plcB_2 and plcB_3 showed low identities with those of M. marinum strains. Therefore, Mycobacterium sp. 012931 has differences on genetic and virulence from M. marinum and may induce different interaction mechanisms between host and pathogen. PMID:24879010

  7. Occurrence of a Thiothrix sp. Attached to Mayfly Larvae and Presence of Parasitic Bacteria in the Thiothrix sp.

    PubMed

    Larkin, J M; Henk, M C; Burton, S D

    1990-02-01

    Larvae of the mayfly (Drunella grandis [Eaton]) from Diamond Fork Creek, Utah, were covered with a heavy growth of the sulfide-oxidizing bacterium Thiothrix. The bacterium did not seem to harm the mayfly, but the Thiothrix trichomes were parasitized by three morphologically distinct bacteria, two of which were cytoplasmic and one of which was probably periplasmic. At least two of the parasites destroyed the cytoplasmic contents of the Thiothrix sp., thus killing the host cell. Attempts to obtain the parasites in pure culture were unsuccessful. PMID:16348112

  8. Occurrence of a Thiothrix sp. Attached to Mayfly Larvae and Presence of Parasitic Bacteria in the Thiothrix sp

    PubMed Central

    Larkin, John M.; Henk, Margaret C.; Burton, Sheril D.

    1990-01-01

    Larvae of the mayfly (Drunella grandis [Eaton]) from Diamond Fork Creek, Utah, were covered with a heavy growth of the sulfide-oxidizing bacterium Thiothrix. The bacterium did not seem to harm the mayfly, but the Thiothrix trichomes were parasitized by three morphologically distinct bacteria, two of which were cytoplasmic and one of which was probably periplasmic. At least two of the parasites destroyed the cytoplasmic contents of the Thiothrix sp., thus killing the host cell. Attempts to obtain the parasites in pure culture were unsuccessful. Images PMID:16348112

  9. Biodegradation of 2,4-dinitrotoluene by a Pseudomonas sp

    SciTech Connect

    Spanggord, R.J.; Mortelmans, K.E. (SRI International, Menlo Park, CA (United States)); Spain, J.C.; Nishino, S.F. (Air Force Engineering and Services Center, Tyndall AFB, FL (United States))

    1991-11-01

    Previous studies of the biodegradation of nonpolar nitroaromatic compounds have suggested that microorganism can reduce the nitro groups but cannot cleave the aromatic ring. The authors report here the initial steps in a pathway for complete biodegradation of 2,4-dinitrotoluene (DNT) by a Pseudomonas sp. isolated from a four-member consortium enriched with DNT. The Pseudomonas sp. degraded DNT as the sole source of carbon and energy under aerobic conditions with stoichiometric release of nitrite. During induction of the enzymes required for growth on DNT, 4-methyl-5-nitrocatechol (MNC) accumulated transiently in the culture fluid when cells grown on acetate were transferred to medium containing DNT as the sole carbon and energy source. Conversion of DNT to MNC in the presence of {sup 18}O{sub 2} revealed the simultaneous incorporation of two atoms of molecular oxygen, which demonstrated that the reaction was catalyzed by a dioxygenase. Fully induced cells degraded MNC rapidly with stoichiometric release of nitrite. The results indicate an initial dioxygenase attack at the 4,5 position of DNT with the concomitant release of nitrite. Subsequent reactions lead to complete biodegradation and removal of the second nitro group as nitrite.

  10. SP-100 power system conceptual design for lunar base applications

    NASA Astrophysics Data System (ADS)

    Mason, Lee S.; Bloomfield, Harvey S.; Hainley, Donald C.

    A conceptual design is presented for a nuclear power system utilizing an SP-100 reactor and multiple Stirling cycle engines for operation on the lunar surface. Based on the results of this study, it was concluded that this power plant could be a viable option for an evolutionary lunar base. The design concept consists of a 2500 kWt (kilowatt thermal) SP-100 reactor coupled to eight free-piston Stirling engines. Two of the engines are held in reserve to provide conversion system redundancy. The remaining engines operate at 91.7 percent of their rated capacity of 150 kWe. The design power level for this system is 825 kWe. Each engine has a pumped heat-rejection loop connected to a heat pipe radiator. Power system performance, sizing, layout configurations, shielding options, and transmission line characteristics are described. System components and integration options are compared for safety, high performance, low mass, and ease of assembly. The power plant was integrated with a proposed human lunar base concept to ensure mission compatibility. This study should be considered a preliminary investigation; further studies are planned to investigate the effect of different technologies on this baseline design.

  11. SP-100 first demonstration flight mission concept design

    NASA Astrophysics Data System (ADS)

    Switick, Dennis; Cowan, Charles; Hoover, Darryl; Marcille, Thomas; Otwell, Robert; Shepard, Neal

    1992-01-01

    A conceptual design study was performed to define a SP-100 Space Reactor Power System (SRPS) compatible with a set of proposed first demonstration flight objectives and constraints. The principal mission objective is the confirmation and demonstration of the SP-100 technology and its design application maturity for space missions. The objective is to be achieved by SRPS assembly, ground acceptance test, launch vehicle integration, and on-orbit demonstration of the full range of SRPS performance capabilities. A proposed second objective is to confirm the functional integration and active interfaces of a state-of-the-art electric propulsion thruster subsystem with an SRPS power source, and to demonstrate its on-orbit performance. Key mission requirements and constraints are the following: - Economical Atlas AS launch into an 1100 km orbit; - SRPS gross output of 30 kWe for 3 year mission lifetime; - Spacecraft mass less than 7000 kg; SRPS less than 2500 kg; - Accommodate users by order of magnitude reduction in SRPS fluences; and - SR-100 program performance & safety requirements apply. An SRPS and spacecraft conceptual design was devised which meets all mission requirements. User ability to meet the adopted radiation limits for the GFS user plane interface, and the use of a larger launch vehicle would allow a significant SRPS mass reduction (400 kg) for early 30 kWe missions.

  12. Biosynthesis of the Apoptolidins in Nocardiopsis sp. FU 40

    PubMed Central

    Du, Yu; Derewacz, Dagmara K.; Deguire, Sean M.; Teske, Jesse; Ravel, Jacques

    2011-01-01

    The apoptolidins are 20/21-membered macrolides produced by Nocardiopsis sp. FU40. Several members of this family are potent and remarkably selective inducers of apoptosis in cancer cell lines, likely via a distinct mitochondria associated target. To investigate the biosynthesis of this natural product, the complete genome of the apoptolidin producer Nocardiopsis sp. FU40 was sequenced and a 116 Kb region was identified containing a putative apoptolidin biosynthetic gene cluster. The apoptolidin gene cluster comprises a type I polyketide synthase, with 13 homologating modules, apparently initiated in an unprecedented fashion via transfer from a methoxymalonyl-acyl carrier protein loading module. Spanning approximately 39 open reading frames, the gene cluster was cloned into a series of overlapping cosmids and functionally validated by targeted gene disruption experiments in the producing organism. Disruption of putative PKS and P450 genes delineated the roles of these genes in apoptolidin biosynthesis and chemical complementation studies demonstrated intact biosynthesis peripheral to the disrupted genes. This work provides insight into details of the biosynthesis of this biologically significant natural product and provides a basis for future mutasynthetic methods for the generation of non-natural apopotolidins. PMID:21869849

  13. Lubrication by the red microalgae Porphyridium sp. polysaccharide

    NASA Astrophysics Data System (ADS)

    Gourdon, Delphine; Lin, Qi; Golan, Yuval

    2004-03-01

    Using a surface forces apparatus (SFA) we have investigated the effects of normal load (compression) and shear on the tribological properties of mica surfaces bearing Porphyridium sp. (a common naturally-occurring polysaccharide of the red microalgae, which molecular weight has been estimated to be 2-7 x 106 daltons) adsorbed from aqueous solution. The friction forces were monitored as a function of time, shearing distance and driving velocity, at different loads and for different polysaccharide concentrations. The friction results indicate a very low coefficient of friction (? <0.01) at low compressions, increasing only to ? =0.015 at pressures of 10 MPa. Its low friction, its ability to resist to high pressure, as well as its weak (logarithmic) dependency of friction on sliding velocity make the Porphyridium sp. a good candidate for biolubrication (e.g. human joint lubrication). Atomic force microscopy measurements were also performed on each surface before and after a shearing experiment and showed ordering of the biopolymer chain molecules into layers over length-scales of several microns.

  14. SP-100 operational life model. Fiscal Year 1990 annual report

    SciTech Connect

    Ewell, R.; Awaya, H.

    1990-12-14

    This report covers the initial year`s effort in the development of an Operational Life Model (OLM) for the SP-100 Space Reactor Power System. The initial step undertaken in developing the OLM was to review all available documentation from GE on their plans for the OLM and on the degradation and failure mechanisms envisioned for the SP-100. In addition, the DEGRA code developed at JPL, which modelled the degradation of the General Purpose Heat Source based Radioisotope Thermoelectric Generator (GPHS-RTG), was reviewed. Based on the review of the degradation and failure mechanisms, a list of the most pertinent degradation effects along with their key degradation mechanisms was compiled. This was done as a way of separating the mechanisms from the effects and allowing all of the effects to be incorporated into the OLM. The emphasis was on parameters which will tend to change performance as a function of time and not on those that are simply failures without any prior degradation.

  15. SpIES:The Spitzer IRAC Equatorial Survey

    NASA Astrophysics Data System (ADS)

    Timlin, John; Ross, Nicholas; Richards, Gordon T.; Lacy, Mark; Bauer, Franz E.; Brandt, W. Niel; Fan, Xiaohui; Haggard, Daryl; Makler, Martin; Myers, Adam D.; Strauss, Michael A.; Urry, C. Megan; SpIES Team

    2015-01-01

    The Spitzer-IRAC Equatorial Survey, SpIES, is an Exploration Science program using Warm Spitzer to map over 100deg^2 of the SDSS Stripe 82 field, and is the largest extragalactic area surveyed by Spitzer. The primary science drivers are: the measurement of z>3 quasar clustering and the luminosity function in order to test different "AGN feedback'' models; to identify obscured AGN (and take advantage of the wide range of multi-wavelength, multi-epoch ancillary data on the Stripe 82 field); to identify z>6 quasars, and to support other wide-field ancillary science. With our observations very recently completed, we present the first preliminary science results from SpIES. This work is based [in part] on observations made with the Spitzer Space Telescope, which is operated by the Jet Propulsion Laboratory, California Institute of Technology under a contract with NASA. Support for this work was provided by NASA through an award issued by JPL/Caltech.

  16. Pseudomonas sp. Strain 273, an Aerobic ?,?-DichloroalkaneDegrading Bacterium

    PubMed Central

    Wischnak, Catrin; Löffler, Frank E.; Li, Jieran; Urbance, John W.; Müller, Rudolf

    1998-01-01

    A gram-negative, aerobic bacterium was isolated from soil; this bacterium grew in 50% (vol/vol) suspensions of 1,10-dichlorodecane (1,10-DCD) as the sole source of carbon and energy. Phenotypic and small-subunit ribosomal RNA characterizations identified the organism, designated strain 273, as a member of the genus Pseudomonas. After induction with 1,10-DCD, Pseudomonas sp. strain 273 released stoichiometric amounts of chloride from C5 to C12 ?,?-dichloroalkanes in the presence of oxygen. No dehalogenation occurred under anaerobic conditions. The best substrates for dehalogenation and growth were C9 to C12 chloroalkanes. The isolate also grew with nonhalogenated aliphatic compounds, and decane-grown cells dechlorinated 1,10-DCD without a lag phase. In addition, cells grown on decane dechlorinated 1,10-DCD in the presence of chloramphenicol, indicating that the 1,10-DCD-dechlorinating enzyme system was also induced by decane. Other known alkane-degrading Pseudomonas species did not grow with 1,10-DCD as a carbon source. Dechlorination of 1,10-DCD was demonstrated in cell extracts of Pseudomonas sp. strain 273. Cell-free activity was strictly oxygen dependent, and NADH stimulated dechlorination, whereas EDTA had an inhibitory effect. PMID:9726906

  17. Domain analysis for estrogen receptor/Sp1-mediated transactivation and detection of estrogen receptor/Sp1 protein interactions in living cells 

    E-print Network

    Kim, KyoungHyun

    2005-11-01

    Estrogen Receptor ? (ER?)/Sp1 activation of GC-rich gene promoters in breast cancer cells is dependent, in part, on the activation function 1 (AF1) of ER?. This study investigates contributions of the DNA binding domain ...

  18. Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov., thermophilic anaerobic representatives of the phylum Planctomycetes.

    PubMed

    Slobodkina, Galina B; Kovaleva, Olga L; Miroshnichenko, Margarita L; Slobodkin, Alexander I; Kolganova, Tatyana V; Novikov, Andrei A; van Heerden, Esta; Bonch-Osmolovskaya, Elizaveta A

    2015-03-01

    Two novel strains of thermophilic planctomycetes were recovered from terrestrial and subterranean habitats. Strain R1(T) was isolated from a hot spring (Kunashir Island, Russia) and strain SBP2(T) was isolated from a deep gold mine (South Africa). Both isolates grew in the temperature range 30-60 °C and pH range 5.0-8.0. Strain R1(T) grew optimally at 60 °C and pH 6.0-6.5; for SBP2(T) optimal conditions were at 52 °C and pH 7.5-8.0. Both strains were capable of anaerobic respiration with nitrate and nitrite as electron acceptors as well as of microaerobic growth. They also could grow by fermentation of mono-, di- and polysaccharides. Based on their phylogenetic position and phenotypic features we suggest that the new isolates represent two novel species belonging to a new genus in the order Planctomycetales, for which the names Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov. are proposed. The type strain of Thermogutta terrifontis, the type species of the genus, is R1(T) (?=?DSM 26237(T)?=?VKM B-2805(T)), and the type strain of Thermogutta hypogea is SBP2(T) (?=?JCM 19991(T)?=?VKM B-2782(T)). PMID:25479950

  19. Gluconacetobacter tumulicola sp. nov. and Gluconacetobacter asukensis sp. nov., isolated from the stone chamber interior of the Kitora Tumulus.

    PubMed

    Tazato, Nozomi; Nishijima, Miyuki; Handa, Yutaka; Kigawa, Rika; Sano, Chie; Sugiyama, Junta

    2012-08-01

    Six Gram-negative, rod-shaped, non-spore-forming bacterial strains were isolated from small holes on plaster walls of the stone chamber interior of the Kitora Tumulus in Asuka village, Nara Prefecture, Japan. These were investigated by means of a polyphasic approach. All the isolates were strictly aerobic and motile by peritrichous flagella. Phylogenetic trees generated based on 16S rRNA gene sequences identified two novel lineages (comprising five isolates and one isolate, respectively) within the genus Gluconacetobacter. The isolates were characterized by having Q-10 as the major ubiquinone system and C(18:1)?7c (58.7-63.1% of the total) as the predominant fatty acid. DNA-DNA hybridization experiments endorsed the species rank for the two lineages, for which the names Gluconacetobacter tumulicola sp. nov. (type strain K5929-2-1b(T)?=?JCM 17774(T)?=?NCIMB 14760(T)) and Gluconacetobacter asukensis sp. nov. (type strain K8617-1-1b(T)?=?JCM 17772(T)?=?NCIMB 14759(T)) are proposed. PMID:22038998

  20. Desulfuromonas svalbardensis sp. nov. and Desulfuromusa ferrireducens sp. nov., psychrophilic, Fe(III)-reducing bacteria isolated from Arctic sediments, Svalbard.

    PubMed

    Vandieken, Verona; Mussmann, Marc; Niemann, Helge; Jørgensen, Bo Barker

    2006-05-01

    Two psychrophilic, Gram-negative, rod-shaped, motile bacteria (strains 112T and 102T) that conserved energy from dissimilatory Fe(III) reduction concomitant with acetate oxidation were isolated from permanently cold Arctic marine sediments. Both strains grew at temperatures down to -2 degrees C, with respective temperature optima of 14 degrees C and 14-17 degrees C for strains 112T and 102T. The isolated strains reduced Fe(III) using common fermentation products such as acetate, lactate, propionate, formate or hydrogen as electron donors, and they also grew with fumarate as the sole substrate. As alternatives to Fe(III), they reduced fumarate, S0 and Mn(IV). Based on 16S rRNA gene sequence similarity, strain 112T was most closely related to Desulfuromonas acetoxidans (97.0 %) and Desulfuromonas thiophila NZ27T (95.5 %), and strain 102T to Malonomonas rubra Gra Mal 1T (96.3 %) and Desulfuromusa succinoxidans GylacT (95.9 %) within the Deltaproteobacteria. Strains 112T and 102T therefore represent novel species, for which the names Desulfuromonas svalbardensis sp. nov. (type strain 112T=DSM 16958T=JCM 12927T) and Desulfuromusa ferrireducens sp. nov. (type strain 102T=DSM 16956T=JCM 12926T) are proposed. PMID:16627667

  1. Rhodococcus sp. strain TM1 plays a synergistic role in the degradation of piperidine by Mycobacterium sp. strain THO100.

    PubMed

    Kim, Yong-Hak; Kang, Un-Beom; Konishi, Kyoko; Lee, Cheolju

    2006-09-01

    Mycobacterium sp. strain THO100 and Rhodococcus sp. strain TM1 were isolated from a morpholine-containing enrichment culture of activated sewage sludge. Strain THO100, but not strain TM1, was able to degrade alicyclic amines such as morpholine, piperidine, and pyrrolidine. The mixed strains THO100 and TM1 showed a better growth on piperidine as the substrate than the pure strain THO100 because strain TM1 was able to reduce the level of glutaraldehyde (GA) produced during piperidine degradation. GA was toxic to strain THO100 (IC(50) = 28.3 microM) but less toxic to strain TM1 (IC(50) = 215 microM). Strain THO100 possessed constitutive semialdehyde dehydrogenases, namely Sad1 and Sad2, whose activities toward succinic semialdehyde (SSA) were strongly inhibited by GA. The two isozymes were identified as catalase-peroxidase (KatG = Sad1) and semialdehyde dehydrogenase (Sad2) based on mass spectrometric analyses of tryptic peptides and database searches of the partial DNA sequences of their genes. In contrast, strain TM1 containing another constitutive enzyme Gad1 could oxidize both SSA and GA. This study suggested that strain TM1 possessing Gad1 played a synergistic role in reducing the toxic and inhibitory effects of GA produced in the degradation of piperidine by strain THO100. PMID:16832627

  2. Kinetics of Acetate Utilization by Two Thermophilic Acetotrophic Methanogens: Methanosarcina sp. Strain CALS-1 and Methanothrix sp. Strain CALS-1.

    PubMed

    Min, H; Zinder, S H

    1989-02-01

    The kinetics of acetate utilization were examined for washed concentrated cell suspensions of two thermophilic acetotrophic methanogens isolated from a 58 degrees C anaerobic digestor. Progress curves for acetate utilization by cells of Methanosarcina sp. strain CALS-1 showed that the utilization rate was concentration independent (zero order) above concentrations near 3 mM and that acetate utilization ceased when a threshold concentration near 1 mM was reached. Acetate utilization by cells of Methanothrix sp. strain CALS-1 was concentration independent down to 0.1 to 0.2 mM, and threshold values of 12 to 21 muM were observed. Typical utilization rates in the concentration-independent stage were 210 and 130 nmol min mg of protein for the methanosarcina and the methanothrix, respectively. These results are in agreement with a general model in which high acetate concentrations favor Methanosarcina spp., while low concentrations favor Methanothrix spp. However, acetate utilization by these two strains did not follow simple Michaelis-Menton kinetics. PMID:16347856

  3. Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica

    USGS Publications Warehouse

    Rodriguez, Russell J.; Connell, L.; Redman, R.; Barrett, A.; Iszard, M.; Fonseca, A.

    2010-01-01

    During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales (Tremellomycetes, Agaricomycotina), but did not correspond to any of the hitherto recognized species. Two novel species, Dioszegia antarctica sp. nov. (type strain ANT-03-116T =CBS 10920T =PYCC 5970T) and Dioszegia cryoxerica sp. nov. (type strain ANT-03-071T =CBS 10919T =PYCC 5967T), are described to accommodate ten and three of these strains, respectively. Analysis of ITS sequences demonstrated intrastrain sequence heterogeneity in D. cryoxerica. The latter species is also notable for producing true hyphae with clamp connections and haustoria. However, no sexual structures were observed. The two novel species can be considered obligate psychrophiles, since they failed to grow above 20?°C and grew best between 10 and 15?°C.

  4. Lutzomyia adiketis sp. n. (Diptera: Phlebotomidae), a vector of Paleoleishmania neotropicum sp. n. (Kinetoplastida: Trypanosomatidae) in Dominican amber

    PubMed Central

    Poinar, George

    2008-01-01

    Background Amber fossils can be used to trace the history of disease-vector associations because microorganisms are preserved "in situ" inside the alimentary tract and body cavity of blood-sucking insects. Results Lutzomyia adiketis sp. n. (Phlebotomidae: Diptera) is described from Dominican amber as a vector of Paleoleishmania neotropicum sp. n. (Kinetoplastida: Trypanosomatidae). The fossil sand fly differs from all previously described extinct and extant members of the genus by the following combination of characters: Sc forked with the branches meeting the costa and radius veins; wing L/W value of 4.1; a ? value of 18; a ratio ?/? value of 0.86, and the shape and size of the spatulate rods on the ninth sternite. The trypanosomatid is characterized by the structure of its promastigotes, amastigotes and paramastigotes and its transmission by an extinct species of sand fly. Conclusion Morphological characters show that the fossil sand fly is a new extinct species and that it is host to a digenetic species of trypanosomatid. This study provides the first fossil evidence that Neotropical sand flies were vectors of trypanosomatids in the mid-Tertiary (20–30 mya). PMID:18627624

  5. Modestobacter lapidis sp. nov. and Modestobacter muralis sp. nov., isolated from a deteriorated sandstone historic building in Salamanca, Spain.

    PubMed

    Trujillo, Martha E; Goodfellow, Michael; Busarakam, Kanungnid; Riesco, Raul

    2015-08-01

    A polyphasic study was undertaken to establish the taxonomic status of two Modestobacter strains isolated from the surface of deteriorated sandstone of a historic building in Salamanca, Spain. The strains, isolates MDVD1(T) and MON 3.1(T), were found to have chemotaxonomic and morphological properties consistent with their classification in the genus Modestobacter and to form distinct phyletic lines in the Modestobacter 16S rRNA gene tree. Isolate MDVD1(T) was found to be closely related to the type strain of Modestobacter versicolor (98.7 % similarity) and isolate MON 3.1(T) to the type strain of Modestobacter multiseptatus (98.6 % similarity). The isolates were distinguished readily from one another and from the Modestobacter type strains by a broad range of phenotypic properties, by qualitative and quantitative differences in fatty acid profiles and by BOX fingerprint patterns. On the basis of these data, it is proposed that the isolates be classified in the genus Modestobacter as Modestobacter lapidis sp. nov. and Modestobacter muralis sp. nov., with isolates MON 3.1(T) (CECT 8844(T) = DSM 100206(T)) and MDVD1(T) (CECT 8845(T) = DSM 100205(T)) as the respective type strains. PMID:25987397

  6. Reconstruction and Comparison of the Metabolic Potential of Cyanobacteria Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803

    PubMed Central

    Saha, Rajib; Verseput, Alex T.; Berla, Bertram M.; Mueller, Thomas J.; Pakrasi, Himadri B.; Maranas, Costas D.

    2012-01-01

    Cyanobacteria are an important group of photoautotrophic organisms that can synthesize valuable bio-products by harnessing solar energy. They are endowed with high photosynthetic efficiencies and diverse metabolic capabilities that confer the ability to convert solar energy into a variety of biofuels and their precursors. However, less well studied are the similarities and differences in metabolism of different species of cyanobacteria as they pertain to their suitability as microbial production chassis. Here we assemble, update and compare genome-scale models (iCyt773 and iSyn731) for two phylogenetically related cyanobacterial species, namely Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803. All reactions are elementally and charge balanced and localized into four different intracellular compartments (i.e., periplasm, cytosol, carboxysome and thylakoid lumen) and biomass descriptions are derived based on experimental measurements. Newly added reactions absent in earlier models (266 and 322, respectively) span most metabolic pathways with an emphasis on lipid biosynthesis. All thermodynamically infeasible loops are identified and eliminated from both models. Comparisons of model predictions against gene essentiality data reveal a specificity of 0.94 (94/100) and a sensitivity of 1 (19/19) for the Synechocystis iSyn731 model. The diurnal rhythm of Cyanothece 51142 metabolism is modeled by constructing separate (light/dark) biomass equations and introducing regulatory restrictions over light and dark phases. Specific metabolic pathway differences between the two cyanobacteria alluding to different bio-production potentials are reflected in both models. PMID:23133581

  7. Biosorption of cadmium, copper, and lead ions from aqueous solutions by Ralstonia sp. and Bacillus sp. isolated from diesel and heavy metal contaminated soil

    Microsoft Academic Search

    Aejung Choi; Sookyun Wang; Minhee Lee

    2009-01-01

    Sorption batch experiments using bacterial strains isolated from soil were performed to remove heavy metals such as Cd, Cu,\\u000a and Pb from an aqueous solution. Ralstonia sp. and Bacillus sp. were used as biosorbents and the distilled water, artificially titrated at 5 mg\\/L for Cd, Cu, and Pb, was used for the\\u000a aqueous solution. Various amounts of biosorbents were mixed

  8. Marine Diatom, Navicula sp. Strain JPCC DA0580 and Marine Green Alga, Chlorella sp. Strain NKG400014 as Potential Sources for Biodiesel Production

    Microsoft Academic Search

    Mitsufumi Matsumoto; Hiroshi Sugiyama; Yoshiaki Maeda; Reiko Sato; Tsuyoshi Tanaka; Tadashi Matsunaga

    2010-01-01

    Marine diatom, strain JPCC DA0580, and marine green microalga strain NKG400014 were selected as high neutral lipid-producers\\u000a from marine microalgal culture collection toward biodiesel production. These strains were tentatively identified as Navicula sp. and Chlorella sp., respectively, by 18S rDNA analysis. Growth and lipid accumulation conditions of both strains were analyzed by changing\\u000a nutrient concentrations in growth media and initial

  9. 1 Estimating aquifer hydraulic properties from the inversion of surface 2 Streaming Potential (SP) anomalies

    E-print Network

    Sailhac, Pascal

    1 Estimating aquifer hydraulic properties from the inversion of surface 2 Streaming Potential (SP with the geometry of the water table. It follows that 11 SP measurements can be used to estimate aquifer hydraulic and found that we 14 are able to estimate the hydraulic conductivity and the depth 15 and the thickness

  10. Draft Genome Sequences of Vibrio sp. Strains Isolated from Tetrodotoxin-Bearing Scavenging Gastropod.

    PubMed

    Kudo, Toshiaki; Kawauchi, Ayumi; Nakahara, Tomomi; Zhang, Xiaochi; Taniyama, Shigeto; Takatani, Tomohiro; Arakawa, Osamu; Oshima, Kenshiro; Suda, Wataru; Kitamura, Keiko; Iida, Toshiya; Iino, Takao; Inoue, Tetsushi; Hongoh, Yuichi; Hattori, Masahira; Ohkuma, Moriya

    2014-01-01

    Vibrio sp. strains JCM 18905 and JCM 19053 were isolated from a tetrodotoxin (TTX)-bearing scavenging gastropod, and Vibrio sp. strain JCM 18904 was isolated from a sea cucumber. All these are closely related to Vibrio alginolyticus. Their comparative genome information is useful for studies of TTX production in bacteria. PMID:24948773

  11. Adsorption of malachite green onto Pithophora sp., a fresh water algae: Equilibrium and kinetic modelling

    Microsoft Academic Search

    K. Vasanth Kumar; S. Sivanesan; V. Ramamurthi

    2005-01-01

    Batch biosorption experiments were carried out for the removal of malachite green a cationic dye from its aqueous solution using raw and thermally activated Pithophora sp., a fresh water algae as biosorbent. The operating variables studied are initial malachite green concentration, biomass concentration and solution pH. Pithophora sp. activated at 300°C for 50min posses a maximum sorption capacity for the

  12. LOVE, M. S., AND M. MOSER. 1976. Dauisia reginae sp. n. (Protozoa: Myxosporida) from

    E-print Network

    LOVE, M. S., AND M. MOSER. 1976. Dauisia reginae sp. n. (Protozoa: Myxosporida) from four, and Washington marine and estuarine fishes. U.s. Dep. Commer., NOAA Tech. Rep. NMFS SSRF-777, 576 p. LOVE, M. S. Soc. Spec. Pub!. 5. MOSER, M., AND M. S. LOVE. 1975. Henneguya sebasta sp. n. (Protozoa, Myxosporida

  13. Evidence for a free-living life stage of the blue crab parasitic dinoflagelate, Hematodinium sp

    Microsoft Academic Search

    M. E. Frischer; R. F. Lee; M. A. Sheppard; A. Mauer; F. Rambow; M. Neumann; J. E. Brofft; T. Wizenmann; J. M. Danforth

    2006-01-01

    Hematodinium sp. is a parasitic dinoflagellate reported to cause disease and death in a variety of crustacean species including the blue crab (Callinectes sapidus). However, because of difficulties in the culture of Hematodinium sp. associated with blue crabs, little is known about its life cycle or mode of transmission. Here, we report the first detection of this organism outside of

  14. SP3X : a six-degree of freedom device for natural model creation

    E-print Network

    Whitney, Richard Henry, III

    2007-01-01

    This thesis presents a novel input device, called SP3X, for the creation of digital models in a semi-immersive environment. The goal of SP3X is to enable novice users to construct geometrically complex three-dimensional ...

  15. Draft Genome Sequence of the Electricigen Acidiphilium sp. Strain PM (DSM 24941)

    PubMed Central

    San Martin-Uriz, Patxi; Gomez, Manuel J.; Arcas, Aida; Bargiela, Rafael; Amils, Ricardo

    2011-01-01

    Acidiphilium sp. strain PM (DSM 24941) was isolated from Rio Tinto's acidic, heavy metal-rich waters. Voltammetry experiments revealed that this strain is capable of electricity production even under aerobic conditions. Here we report the draft genome sequence of Acidiphilium sp. PM and a preliminary genome analysis that reveals a versatile respiratory metabolism. PMID:21914891

  16. Genome sequence of Pantoea sp. strain Sc 1 an opportunistic cotton pathogen

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pantoea is comprised of a broad spectrum of species including plant pathogens. Here, we provide an annotated genome sequence of Pantoea sp. strain Sc 1, which was isolated from a diseased cotton boll. This research provides the first genome sequence of a bona fide Pantoea sp. insect vectored cotton...

  17. Arbuscular mycorrhizal fungi colonize decomposing leaves of Myrica parvifolia , M. pubescens and Paepalanthus sp

    Microsoft Academic Search

    Catalina Aristizábal; Emma Lucía Rivera; David P. Janos

    2004-01-01

    Hyphae and vesicles of arbuscular mycorrhizal fungi (AMF) were found within the decomposing leaves of Myrica parvifolia, M. pubescens and Paepalanthus sp. at three montane sites in Colombia. Hyphae, vesicles, and arbuscule-like structures were also found within scale-like leaves of the rhizomes of Paepalanthus sp. The litter found in the vicinity of the roots was divided into three decomposition layers.

  18. Ecological observations on the colonial ascidian Didemnum sp. in a New England tide pool habitat

    Microsoft Academic Search

    Page C. Valentine; Mary R. Carman; Dann S. Blackwood; Erin J. Heffron

    2007-01-01

    The colonial ascidian Didemnum sp. has colonized northwestern Atlantic coastal habitats from southern Long Island, New York, to Eastport, Maine. It is also present in offshore habitats of the Georges Bank fishing grounds. It threatens to alter fisheries habitats and shellfish aquacultures.Observations in a tide pool at Sandwich, MA from December 2003 to February 2006 show that Didemnum sp. tolerates

  19. Draft Genome of Pseudomonas sp. Strain 11/12A, Isolated from Lake Washington Sediment

    PubMed Central

    McTaggart, Tami L.; Shapiro, Nicole; Woyke, Tanja

    2015-01-01

    We announce here the genome sequencing of Pseudomonas sp. strain 11/12A from Lake Washington sediment. From the genome content, a versatile lifestyle is predicted but not one of bona fide methylotrophy. With the availability of its genomic sequence, Pseudomonas sp. 11/12A presents a prospective model for studying microbial communities in lake sediments. PMID:25700412

  20. Draft Genome of Janthinobacterium sp. RA13 Isolated from Lake Washington Sediment

    PubMed Central

    McTaggart, Tami L.; Shapiro, Nicole; Woyke, Tanja

    2015-01-01

    Sequencing the genome of Janthinobacterium sp. RA13 from Lake Washington sediment is announced. From the genome content, a versatile life-style is predicted, but not bona fide methylotrophy. With the availability of its genomic sequence, Janthinobacterium sp. RA13 presents a prospective model for studying microbial communities in lake sediments. PMID:25676775

  1. Graphene-on-Diamond Devices with Increased Current-Carrying Capacity: Carbon sp2

    E-print Network

    Graphene-on-Diamond Devices with Increased Current-Carrying Capacity: Carbon sp2 -on-sp3 Technology Laboratory, Illinois 60439, United States *S Supporting Information ABSTRACT: Graphene demonstrated potential for practical applications owing to its excellent electronic and thermal properties. Typical graphene field

  2. VEMBU STOREGRID SP3.1 SETUP (WINDOWS) 04/22/2010 Page 1 of 23

    E-print Network

    Wang, Hai

    VEMBU STOREGRID SP3.1 SETUP (WINDOWS) 04/22/2010 Page 1 of 23 Title Vembu StoreGrid SP 3.1 set up guide for Windows PCs. Vembu StoreGrid is the Viterbi IT adapted solution for Online Remote Backup for your Windows operating system at http

  3. Pheidole soesilae sp. nov. from Suriname (Hymenoptera: Formicidae) by Dr Dewanand Makhan*

    E-print Network

    Villemant, Claire

    Pheidole soesilae sp. nov. from Suriname (Hymenoptera: Formicidae) by Dr Dewanand Makhan* *Willem Suriname (Hymenoptera: Formicidae). Calodema Supplementary Paper No. 59: 1-2. Abstract: A new Pheidole species (Hymenoptera: Formicidae) is described from Suriname, namely Pheidole soesilae sp. nov. A key

  4. New Pigments from the Terrestrial Cyanobacterium Scytonema sp. Collected on the Mitaraka Inselberg, French Guyana

    E-print Network

    Paris-Sud XI, Université de

    1 New Pigments from the Terrestrial Cyanobacterium Scytonema sp. Collected on the Mitaraka of the ultraviolet-screening, photostable sheath pigment scytonemin. The organic extract of Scytonema sp., collected on the Mitaraka inselberg, French Guyana, yielded three new pigments, tetramethoxyscytonemin (1

  5. Sp100 Interacts with ETS1 and Stimulates Its Transcriptional Activity

    Microsoft Academic Search

    Christine Wasylyk; Sophie E. Schlumberger; Paola Criqui-Filipe; Bohdan Wasylyk

    2002-01-01

    The cell nucleus is highly organized into distinct domains that spatially separate physiological processes. One of these domains, the Sp100-promyelocytic leukemia protein nuclear body (NB), is implicated in patho- logical processes, such as cancer and viral infection, yet its functions remain poorly understood. We show here that Sp100 interacts physically and functionally with ETS-1 and that NB morphology is affected

  6. Potassium uptake in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 mainly depends on

    E-print Network

    Roegner, Matthias

    Potassium uptake in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 mainly depends First published online 3 July 2003 Edited by Stuart Ferguson Abstract The molecular basis of potassium potassium transporters can be identi¢ed in the genome of Synechocystis sp. strain PCC 6803. Mutants

  7. Draft Genome Sequence of Antimicrobial-Producing Clostridium sp. JC272, Isolated from Marine Sediment

    PubMed Central

    Tushar, L.; Sasi Jyothsna, T. S.; Ramana, C. V.

    2015-01-01

    We announce the draft genome sequence of Clostridium sp. JC272, isolated from a sediment sample collected from marine habitats of Gujarat, India. Clostridium sp. JC272 is an obligate anaerobe and has the ability to produce antimicrobial compounds. The genome sequence indicates the strain’s capability of producing small peptides (microcins), which are potential novel antibiotics. PMID:26067979

  8. Selective enrichment broth for the isolation of Aeromonas sp. from chicken meat

    Microsoft Academic Search

    N Sachan; R. K Agarwal

    2000-01-01

    Six selective agents (ampicillin, novobiocin, cephalothin, bile salts, brilliant green and ethanol) were tested during the development of a selective enrichment broth for the isolation of Aeromonas sp. from food. Cephalothin at 10 mg\\/l was found to be the best selective agent owing to its greater selectivity and efficiency in recovering stressed and lower cell concentrations of Aeromonas sp. Higher

  9. Renal coccidiosis caused by Eimeria gaviae n. sp. in a common loon (Gavia immer).

    PubMed

    Montgomery, R D; Novilla, M N; Shillinger, R B

    1978-01-01

    Renal coccidiosis was diagnosed in a common loon (Gavia immer). This is the first report of coccidium from the kidney of a member of the Order Gaviiformes. Morphologically, the coccidium was considered to be an Eimeria sp. distinct from Eimeria truncata, and was named Eimeria gaviae n. sp. PMID:749899

  10. An Integration of Concept Structure Analysis and SP Chart with Application in Equality Axiom Concepts Diagnosis

    Microsoft Academic Search

    Yuan-Horng Lin; Jeng-Ming Yih

    2008-01-01

    The purpose of this study is to discuss an integrated method which could provide diagnostic information for items and students. This integrated method combined two major methods. One is concept structure analysis and the other is student problem chart (S-P chart). The concept structure analysis could analyze individualized concepts structure and S-P chart could classify students and items into proper

  11. American Arachnological Society Ontogenetic Changes in the Web of Epeirotypus sp. (Araneae, Theridiosomatidae)

    E-print Network

    Bermingham, Eldredge

    American Arachnological Society Ontogenetic Changes in the Web of Epeirotypus sp. (Araneae & Conditions of Use, available at . http://www.jstor.org/page/info/about/policies/terms.jsp JSTOR is a not IN THE WEB OF EPEIROTYPUS SP. (ARANEAE, THERIDIOSOMATIDAE) A recent summary of information on changes

  12. Genome Sequence of Blattabacterium sp. Strain BGIGA, Endosymbiont of the Blaberus giganteus Cockroach

    PubMed Central

    Huang, Charlie Y.; Moran, Nancy A.

    2012-01-01

    Cockroaches harbor the obligate flavobacterial endosymbiont Blattabacterium sp., which resides within the host's bacteriocytes and can recycle ammonia and urea nitrogenous wastes into amino acids for the host. We report the complete genome sequence of the Blattabacterium sp. associated with the giant roach Blaberus giganteus. PMID:22843586

  13. The Lewis heat pipe code with application to SP100 GES heat pipes

    Microsoft Academic Search

    Karl W. Baker; Leonard K. Tower

    1988-01-01

    The NASA Lewis Research Center has a thermal management program supporting SP-100 goals, which includes heat pipe radiator development. As a part of the program Lewis has elected to prepare an in-house heat pipe code tailored to the needs of its SP-100 staff to supplement codes from other sources. The latter, designed to meet the needs of the originating organizations,

  14. Selective production of glutamate by an immobilized marine blue-green alga, Synechococcus sp

    Microsoft Academic Search

    Tadashi Matsunaga; Noriyuki Nakamura; Naoko Tsuzaki; Hiroyuki Takeda

    1988-01-01

    Among 200 strains of marine bluegreen algae isolated from the coastal areas of Japan, the marine blue-green alga Synechococcus sp. NKBG 040607 excreted glutamate at the highest rate, 82.6% of total amino acids production being glutamate. Synechococcus sp. NKBG 40607 was immobilized in calcium alginate gel. Glutamate production by immobilized cells was double that of native cells. Maximal glutamate production

  15. Single cell oil production by Gordonia sp. DG using agro-industrial wastes

    Microsoft Academic Search

    Mona K. Gouda; Sanaa H. Omar; Linda M. Aouad

    2008-01-01

    Lipid accumulation by Gordonia sp. DG using sodium gluconate as carbon source in comparison with Rhodococcus opacus PD630 was studied. Maximum lipid content 80% was observed at the beginning of the stationary phase for R. opacus and 72% at the end of stationary phase for Gordonia sp. Different agro-industrial wastes were used as carbon source. The cells of the two

  16. In vitro antibacterial and anti-inflammatory effects of honokiol and magnolol against Propionibacterium sp

    Microsoft Academic Search

    Junho Park; Jongsung Lee; Eunsun Jung; Yumi Park; Kukhyun Kim; Kwangseon Jung; Jieun Kim

    2004-01-01

    Honokiol and magnolol, two major phenolic constituents of Magnolia sp., have been known to exhibit antibacterial activities. However, until now, their antibacterial activity against Propionibacterium sp. has not been reported. To this end, the antibacterial activities of honokiol and magnolol were detected using the disk diffusion method and a two-fold serial dilution assay. Honokiol and magnolol showed strong antibacterial activities

  17. The influence of salinity variation on Hematodinium sp. infection of blue crabs in the ACE Basin

    E-print Network

    Childress, Michael J.

    The influence of salinity variation on Hematodinium sp. infection of blue crabs in the ACE Basin measured salinity, crab abundance, fishing effort, and Hematodinium sp. prevalence at 27 locations and extracted using a Chelex solution. PCR was used to amplify crab DNA using a Tubulin primer pair

  18. The life cycle and host specificity of Psychodiella sergenti n. sp. and Ps. tobbi n. sp. (Protozoa: Apicomplexa) in sand flies Phlebotomus sergenti and Ph. tobbi (Diptera: Psychodidae).

    PubMed

    Lantová, Lucie; Ghosh, Kashinath; Svobodová, Milena; Braig, Henk R; Rowton, Edgar; Weina, Peter; Volf, Petr; Votýpka, Jan

    2010-10-01

    Two new gregarines in the recently erected genus Psychodiella (formerly Ascogregarina), Psychodiella sergenti n. sp. and Psychodiella tobbi n. sp., are described based on morphology and life cycle observations conducted on larvae and adults of their natural hosts, the sand flies Phlebotomus sergenti and Phlebotomus tobbi, respectively. The phylogenetic analyses inferred from small subunit ribosomal DNA (SSU rDNA) sequences indicate the monophyly of newly described species with Psychodiella chagasi. Ps. sergenti n. sp. and Ps. tobbi n. sp. significantly differ from each other in the life cycle and in the size of life stages. The sexual development of Ps. sergenti n. sp. (syzygy, formation of gametocysts and oocysts) takes place exclusively in blood-fed Ph. sergenti females, while the sexual development of Ps. tobbi n. sp. takes place also in males and unfed females of Ph. tobbi. The susceptibility of Phlebotomus perniciosus, Phlebotomus papatasi, Ph. sergenti, Ph. tobbi, and Phlebotomus arabicus to both gregarines was examined by exposing 1st instar larvae to parasite oocysts. High host specificity was observed, as both gregarines were able to fully develop and complete regularly the life cycle only in their natural hosts. Both gregarines are considered as serious pathogens in laboratory-reared colonies of Old World sand flies. PMID:20627106

  19. Ethyl tert-butyl ether (ETBE) biodegradation by a syntrophic association of Rhodococcus sp. IFP 2042 and Bradyrhizobium sp. IFP 2049 isolated from a polluted aquifer.

    PubMed

    Le Digabel, Yoann; Demanèche, Sandrine; Benoit, Yves; Vogel, Timothy M; Fayolle-Guichard, Françoise

    2013-12-01

    Ethyl tert-butyl ether (ETBE) enrichment was obtained by adding contaminated groundwater to a mineral medium containing ETBE as the sole carbon and energy source. ETBE was completely degraded to biomass and CO2 with a transient production of tert-butanol (TBA) and a final biomass yield of 0.37 ± 0.08 mg biomass (dry weight).mg(-1) ETBE. Two bacterial strains, IFP 2042 and IFP 2049, were isolated from the enrichment, and their 16S rRNA genes (rrs) were similar to Rhodococcus sp. (99 % similarity to Rhodococcus erythropolis) and Bradyrhizobium sp. (99 % similarity to Bradyrhizobium japonicum), respectively. Rhodococcus sp. IFP 2042 degraded ETBE to TBA, and Bradyrhizobium sp. IFP 2049 degraded TBA to biomass and CO2. A mixed culture of IFP 2042 and IFP 2049 degraded ETBE to CO2 with a biomass yield similar to the original ETBE enrichment (0.31 ± 0.02 mg biomass.mg(-1) ETBE). Among the genes previously described to be involved in ETBE, MTBE, and TBA degradation, only alkB was detected in Rhodococcus sp. IFP 2042 by PCR, and none were detected in Bradyrhizobium sp. IFP 2049. PMID:23474617

  20. Conspicuous epiphytic growth of an interspecific hybrid Neotyphodium sp. endophyte on distorted host inflorescences.

    PubMed

    Christensen, Michael John; Saulsbury, Keith; Simpson, Wayne R

    2012-01-01

    Selected Neotyphodium sp. endophytes are now commonly used to enhance pasture persistence and livestock productivity, with seed of perennial ryegrass and tall fescue cultivars with these selected endophytes being commercially available. In a large population of perennial ryegrass plants infected with a Neotyphodium sp. endophyte that was being grown for seed production a small percentage of inflorescences were distorted and covered with a conspicuous white mycelial growth. Within individual plants only a small number of inflorescences were affected and the amount of distortion differed between affected inflorescences. This Neotyphodium sp. is an interspecific hybrid of Epichloë typhina and Neotyphodium. lolii and like nearly all other Neotyphodium spp is symptomless in host grasses. The fungus isolated from distorted inflorescences had colonies that were identical to those isolated from symptomless inflorescences and these were characteristic of this Neotyphodium sp. This is the first report of distorted inflorescences covered with epiphytic hyphal growth on host grasses infected with an interspecific hybrid Neotyphodium sp. PMID:22208600

  1. The morphology and pathology of Besnoitia sp. in reindeer (Rangifer tarandus tarandus).

    PubMed

    Ayroud, M; Leighton, F A; Tessaro, S V

    1995-07-01

    Four of five reindeer (Rangifer tarandus tarandus) obtained from a Besnoitia sp.- infected herd at the Assiniboine Park Zoo in Winnipeg, Manitoba, Canada, in October 1989, had evidence of mild dermatitis over the articular surfaces of carpal and tarsal joints. Cysts of Besnoitia sp., either surrounded by inflammatory reactions or without evident host response, were present within the dermis, submucosa of the nasal turbinates, periosteum, tendons, testes and hooves. The light microscopic and histochemical features of Besnoitia sp. from reindeer were indistinguishable from those of other Besnoitia spp. described in cattle, rodents and horses. The Besnoitia sp. cysts and organisms from reindeer were unique in that bradyzoite membrane micropores and cytoplasmic enigmatic bodies were not observed. Two cats were fed cysts of Besnoitia sp. but no oocysts were detected in feces for 90 days post-infection. PMID:8592351

  2. Prevalence of Giardia sp. in a beaver colony and the resulting environmental contamination.

    PubMed

    Monzingo, D L; Hibler, C P

    1987-10-01

    The prevalence of Giardia sp. in a beaver (Castor canadensis) colony in Colorado was determined by the collection and analysis of fecal samples over a period of 14 mo. Environmental contamination was monitored through the use and analysis of water filter samples. Beaver shed cysts of Giardia sp. in their feces throughout the year with temporal variations in the prevalence, and became infected as kits and remained infected as juveniles and adults. Beaver served as amplification hosts for Giardia sp. and contaminated surface waters downstream from their dams in late spring and early fall. In slow moving waters the cysts of Giardia sp. settled rapidly. Muskrats (Ondatra zibethicus) were the only other species of wildlife shedding cysts of Giardia sp. on the study area. PMID:3682082

  3. Mucopolysaccharidosis Type II and the G374sp Mutation

    PubMed Central

    Martínez-Quintana, E.; Rodríguez-González, F.

    2013-01-01

    Mucopolysaccharidosis type II (MPS II), also known as Hunter syndrome, is a rare, X-linked disease caused by a deficiency of the lysosomal enzyme iduronate-2-sulfatase, which catalyses a step in the catabolism of glycosaminoglycans resulting in accumulation of heparan and dermatan sulfate in many organs and tissues. This accumulation favors the appearance of neurologic involvement, severe airway obstruction, skeletal deformities, and cardiomyopathy, especially mitral and aortic valve regurgitation. In severe cases, obstructive airway disease and cardiac failure due to valvular dysfunction are the most common causes of death within the second decade of life. However, in mild cases, intelligence remains normal, stature is almost normal and death usually occurs due to cardiac failure in the fourth decade of life. We report the presentation, diagnosis, management, and outcome of 2 siblings with MPS II and the G374sp mutation at the nucleotide c.1246 of the gene encoding for the iduronate-2-sulfatase. PMID:23801937

  4. Spacecraft and mission design for the SP-100 flight experiment

    SciTech Connect

    Deininger, W.D.; Vondra, R.J.

    1988-01-01

    The design and performance of a spacecraft employing arcjet nuclear electric propulsion, suitable for use in the SP-100 Space Reactor Power System (SRPS) Flight Experiment, are outlined. The vehicle design is based on a 93 kW(e) ammonia arcjet system operating at an experimentally measured specific impulse of 1031 s and an efficiency of 42.3 percent. The arcjet/gimbal assemblies, power conditioning subsystem, propellant feed system, propulsion system thermal control, spacecraft diagnostic instrumentation, and the telemetry requirements are described. A 100 kW(e) SRPS is assumed. The spacecraft mass is baselined at 5675 kg excluding the propellant and propellant feed system. Four mission scenarios are described which are capable of demonstrating the full capability of the SRPS. The missions considered include spacecraft deployment to possible surveillance platform orbits, a spacecraft storage mission, and an orbit raising round trip corresponding to possible orbit transfer vehicle (OTV) missions.

  5. Identification of Oxaphenalenone Ketals from the Ascomycete Fungus Neonectria sp.

    PubMed

    Ren, Jinwei; Niu, Shubing; Li, Li; Geng, Zhufeng; Liu, Xingzhong; Che, Yongsheng

    2015-06-26

    Neonectrolides B-E (4-7), four new oxaphenalenone ketals incorporating the new furo[2,3-b]isochromeno[3,4,5-def]chromen-11(6aH)-one skeleton, were isolated from the fermentation extract of the ascomycete fungus Neonectria sp. in an in-depth investigation guided by HPLC fingerprint and a cytotoxicity assay. The previously identified oxaphenalenone spiroketal neonectrolide A (1) and its putative biosynthetic precursors (2 and 3) were also reisolated in the current work. The structures of 4-7 were primarily elucidated by interpretation of NMR spectroscopic data, and the absolute configurations were deduced by electronic circular dichroism calculations. Compound 6 showed cytotoxic effects against four of the six human tumor cell lines tested. Biosynthetically, compounds 4-7 could be derived via the Diels-Alder reaction cascades starting from derivatives of the co-isolated metabolites 2 and 3. PMID:25978132

  6. Streptomyces graminifolii sp. nov., isolated from bamboo (Sasa borealis) litter.

    PubMed

    Lee, Hyo-Jin; Whang, Kyung-Sook

    2014-08-01

    The taxonomic position of strain JL-22(T), isolated from litter of a bamboo (Sasa borealis) forest, was determined using a polyphasic approach. The organism had phenotypic and morphological properties consistent with it being a member of the genus Streptomyces. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain JL-22(T) was closely related to Streptomyces prunicolor NRRL B-12281(T) (99.2%), Streptomyces galilaeus JCM 4757(T) (99.0%) and Streptomyces chartreusis NBRC 12753(T) (99.0%). However, the results of DNA-DNA hybridization and physiological and biochemical tests showed that strain JL-22(T) could be differentiated from its closest phylogenetic relatives both genotypically and phenotypically. Based on phenotypic and genotypic data, strain JL-22(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces graminifolii sp. nov. is proposed. The type strain is JL-22(T) (?=?KACC 17180(T)?=?NBRC 109806(T)). PMID:24812360

  7. Power plant system assessment. Final report. SP-100 Program

    SciTech Connect

    Anderson, R.V.; Atkins, D.F.; Bost, D.S.; Berman, B.; Clinger, D.A.; Determan, W.R.; Drucker, G.S.; Glasgow, L.E.; Hartung, J.A.; Harty, R.B.

    1983-10-31

    The purpose of this assessment was to provide system-level insights into 100-kWe-class space reactor electric systems. Using these insights, Rockwell was to select and perform conceptual design studies on a ''most attractive'' system that met the preliminary design goals and requirements of the SP-100 Program. About 4 of the 6 months were used in the selection process. The remaining 2 months were used for the system conceptual design studies. Rockwell completed these studies at the end of FY 1983. This report summarizes the results of the power plant system assessment and describes our choice for the most attractive system - the Rockwell SR-100G System (Space Reactor, 100 kWe, Growth) - a lithium-cooled UN-fueled fast reactor/Brayton turboelectric converter system.

  8. Iron reduction by the cyanobacterium Synechocystis sp. PCC 6803.

    PubMed

    Thorne, Rebecca J; Schneider, Kenneth; Hu, Huaining; Cameron, Petra J

    2015-10-01

    Synechocystis sp. PCC 6803 uptakes iron using a reductive mechanism, similar to that exhibited by many other microalgae. Various bio-electrochemical technologies have made use of this reductive cellular capacity, but there is still a lack of fundamental understanding of cellular reduction rates under different conditions. This study used electrochemical techniques to further investigate the reductive interactions of Synechocystis cells with Fe(III) from the iron species potassium ferricyanide, with varying cell and ferricyanide concentrations present. At the lowest cell concentrations tested, cell reduction machinery appeared to kinetically limit the reduction reaction, but ferricyanide reduction rates were mass transport controlled at the higher cell and ferricyanide concentrations studied. Improving the understanding of the reduction of Fe(III) by whole cyanobacterial cells is important for improving the efficiencies of technologies that rely on this interaction. PMID:26079619

  9. Supersymmetric SP(N) spin representation for heavy fermion systems

    NASA Astrophysics Data System (ADS)

    Ramires, Aline; Coleman, Piers

    2014-03-01

    In heavy fermion materials, the character of the spin correlations change radically between the antiferromagnetic and fermi liquid region of the phase diagram. In the latter, the spin behaves as a bosonic object, condensing into magnetic order, which we traditionally describe using a Holstein-Primakoff or Schwinger bosons, whereas in the the fermi liquid phase, the spin binds to the conduction electrons to form a composite heavy fermion, usually described by Abriskosov fermions. Past work developed a supersymmetric representation of SU(N) spin operators. Here we analyze the supersymmetry of SP(N) symplectic spin operators, which provides us with the capability of studying antiferromagnetic and superconducting order. As a warm up problem, we show how this formalism can be applied to a two site Kondo model, coupled via a Heisenberg coupling. This work is supported by NSF grant DMR 1309929.

  10. Cytotoxic Spliceostatins from Burkholderia sp. and Their Semisynthetic Analogues.

    PubMed

    He, Haiyin; Ratnayake, Anokha S; Janso, Jeffrey E; He, Min; Yang, Hui Y; Loganzo, Frank; Shor, Boris; O'Donnell, Christopher J; Koehn, Frank E

    2014-08-01

    The spliceostatin class of natural products was reported to be potent cytotoxic agents via inhibition of the spliceosome, a key protein complex in the biosynthesis of mature mRNA. As part of an effort to discover novel leads for cancer chemotherapy, we re-examined this class of compounds from several angles, including fermentation of the producing strains, isolation and structure determination of new analogues, and semisynthetic modification. Accordingly, a group of spliceostatins were isolated from a culture broth of Burkholderia sp. FERM BP-3421, and their structures identified by analysis of spectroscopic data. Semisynthesis was performed on the major components 4 and 5 to generate ester and amide derivatives with improved in vitro potency. With their potent activity against tumor cells and unique mode of action, spliceostatins can be considered potential leads for development of cancer drugs. PMID:25098528

  11. Kerstersia similis sp. nov., isolated from human clinical samples.

    PubMed

    Vandamme, Peter; De Brandt, Evie; Houf, Kurt; De Baere, Thierry

    2012-09-01

    Analysis of gyrB gene sequences, (GTG)(5)-primed PCR fingerprinting and biochemical characteristics determined in the Biolog GEN III microtest system were used to differentiate an unnamed Kerstersia species from Kerstersia gyiorum, the type and only named species in this genus. The inability to oxidize D-galacturonic and D-glucuronic acids and the ability to oxidize D-serine, along with gyrB gene sequence analysis and (GTG)(5)-PCR fingerprints, readily differentiated the unnamed taxon from the type species. Therefore, we propose to formally classify this unnamed taxon as Kerstersia similis sp. nov. with strain LMG 5890(T) (= CCUG 46999(T)), isolated from a leg wound in the USA in 1983, as the type strain. PMID:22058318

  12. SP-100 Fuel Pin Performance: Results from Irradiation Testing

    NASA Astrophysics Data System (ADS)

    Makenas, Bruce J.; Paxton, Dean M.; Vaidyanathan, Swaminathan; Marietta, Martin; Hoth, Carl W.

    1994-07-01

    A total of 86 experimental fuel pins with various fuel, liner, and cladding candidate materials have been irradiated in the Experimental Breeder Reactor-II (EBR-II) and the Fast Flux Test Facility (FFTF) reactor as part of the SP-100 fuel pin irradiation testing program. Postirradiation examination results from these fuel pins are key in establishing performance correlations and demonstrating the lifetime and safety of the reactor fuel system. This paper provides a brief description of the in-reactor fuel pin tests and presents the most recent irradiation data on the performance of wrought rhenium (Re) liner material and high density UN fuel at goal burnup of 6 atom percent (at. %). It also provides an overview of the significant variety of other fuel/liner/cladding combinations which were irradiated as part of this program and which may be of interest to more advanced efforts.

  13. Using nano-chitosan for harvesting microalga Nannochloropsis sp.

    PubMed

    Farid, Mohammad Sadegh; Shariati, Ahmad; Badakhshan, Amir; Anvaripour, Bagher

    2013-03-01

    In this study, chitosan and nano-chitosan were used as flocculants agents for harvesting microalga Nannochloropsis sp. chitosan was modified to nano-chitosan by crosslinking with sodium tripolyphosphate. The effects of type and dosage of flocculants and the pH of the culture were investigated on biomass recovery. Optimum dosages for both bio-flocculants were found. The results showed that the dosage of flocculant consumption decreases by 40% and biomass recovery increases by 9% when nano-chitosan instead of chitosan is used as flocculant agent. Also, the recycled water from the harvesting process was reused which increases the growth of microalgae by about 7%. Finally, the cost analysis of harvesting process showed the feasibility of using nano-chitosan as flocculation agent. PMID:23415940

  14. Release of polysaccharide by sonication of cells ( Porphyridium sp.)

    NASA Astrophysics Data System (ADS)

    Faerman, V.; Mukmenev, I.; Shreiber, I.

    2009-03-01

    The paper deals with experimental data concerning the interaction of acoustical waves with microbial cell (Porphyridium sp.). The aim of the present paper was to increase the amount of biopolymer released from the microorganisms biomass with the aid ultrasound irradiation without scission or a decrease in the molecular weight. The results indicated that the amount of polysaccharide (for example) released from the cell pellet could be enhanced by ultrasound, depending on the frequency and energy of the ultrasound. The sugar composition remain the same, but the apparent viscosity of polysaccharide aqueous solutions decreased, indicated that some changes in the molecular shape and size occurred. When ultrasound irradiation was applied in the presence of either CO2 or CO2 + H2, the apparent viscosity of polysaccharide aqueous solutions increased (versus usual ultrasound treatment).

  15. Desulfurization of dibenzothiophene by Corynebacterium sp. strain SY1

    SciTech Connect

    Omori, Toshio; Monna, L.; Saiki, Yuko; Kodama, Tohru (Univ. of Tokyo (Japan))

    1992-03-01

    Strain SY1, identified as a Corynebacterium sp., was isolated on the basis of the ability to utilize dibenzothiophene (DBT) as a sole source of sulfur. Strain SY1 could utilize a wide range of organic and inorganic sulfur compounds, such as DBT sulfone, dimethyl sulfide, dimethyl sulfoxide, dimethyl sulfone, CS{sub 2}, FeS{sub 2}, and even elemental sulfur. Strain SY1 metabolized DBT to dibenzothiophene-5-oxide, DBT sulfone, and 2-hydroxybiphenyl, which was subsequently nitrated to produce at least two different hydroxynitrobiphenyls during cultivation. These metabolites were separated by silica gel column chromatography and identified by nuclear magnetic resonance, UV, and mass spectral techniques. Resting cells of SY1 desulfurized toluenesulfonic acid and released sulfite anion. On the basis of these results, a new DBT degradation pathway is proposed.

  16. Preparation of immobilized microorganism (B. SP 3012) by ?-irradiation

    NASA Astrophysics Data System (ADS)

    Tung-Fu, Lin

    Gel entrapment using ?-ray irradiation provides an efficient method to prepare immobilized fumarase-producing microorganism (B.SP 3012). In the use of such immobilized microorganism for producing L-malic acid from fumaric acid, a high value of the activity of fumarase (13000 ?m/h/g) has been obtained. The enzymatic reaction has been carried out at pH = 6.5 and 37°C. In the case of the column method optimum reaction condition for conversion of fumaric acid to L-malic acid can be easily obtained by adjusting the space velocity of the substrate solution. Due to the sufficient durability of enzymatic activity, the immobilized microorganism can be used repeatedly.

  17. Complete genome sequence of Arthrobacter sp. strain FB24

    SciTech Connect

    Nakatsu, C. H.; Barabote, Ravi; Thompson, Sue; Bruce, David; Detter, Chris; Brettin, T.; Han, Cliff F.; Beasley, Federico; Chen, Weimin; Konopka, Allan; Xie, Gary

    2013-09-30

    Arthrobacter sp. strain FB24 is a species in the genus Arthrobacter Conn and Dimmick 1947, in the family Micrococcaceae and class Actinobacteria. A number of Arthrobacter genome sequences have been completed because of their important role in soil, especially bioremediation. This isolate is of special interest because it is tolerant to multiple metals and it is extremely resistant to elevated concentrations of chromate. The genome consists of a 4,698,945 bp circular chromosome and three plasmids (96,488, 115,507, and 159,536 bp, a total of 5,070,478 bp), coding 4,536 proteins of which 1,257 are without known function. This genome was sequenced as part of the DOE Joint Genome Institute Program.

  18. Chemical control of electrical contact to sp2 carbon atoms

    NASA Astrophysics Data System (ADS)

    Frederiksen, Thomas; Foti, Giuseppe; Scheurer, Fabrice; Speisser, Virginie; Schull, Guillaume

    2014-04-01

    Carbon-based nanostructures are attracting tremendous interest as components in ultrafast electronics and optoelectronics. The electrical interfaces to these structures play a crucial role for the electron transport, but the lack of control at the atomic scale can hamper device functionality and integration into operating circuitry. Here we study a prototype carbon-based molecular junction consisting of a single C60 molecule and probe how the electric current through the junction depends on the chemical nature of the foremost electrode atom in contact with the molecule. We find that the efficiency of charge injection to a C60 molecule varies substantially for the considered metallic species, and demonstrate that the relative strength of the metal-C bond can be extracted from our transport measurements. Our study further suggests that a single-C60 junction is a basic model to explore the properties of electrical contacts to meso- and macroscopic sp2 carbon structures.

  19. [Performance of demulsification by Rhodococcus sp. PR-1].

    PubMed

    Ma, Ting; Liang, Feng-lai; Xi, Yan-wei; Liu, Ru-lin

    2006-06-01

    Rhodococcus sp. PR-1 with high capability of demulsification on a surfactant-stabilized kerosene-water model emulsion was isolated from sewage of Dagang oil field. It could demulsify the model emulsion completely in 8 hours at 55 degrees C and had better demulsifying capability than chemical demulsifier DGF-01. The freezing-thawing and autoclaving had no effect on the process of demulsification, yet ultrasonic disposal and deal with organic solvent could inhibit its activity. A linear relationship, not the first order reaction that was used in some references, was observed between the percentage of demulsification and reaction time. The demulsifying capability of PR-1 was mainly resulted from the hydrophobic surfaces of microbial cells, which were characterized by the mycolic acids with the carbon-chain-length from 27 to 54. These results provide foundation for biological application on demulsifying crude oil emulsions in produced water of oil field. PMID:16921960

  20. Bacillus salexigens sp. nov., a new moderately halophilic Bacillus species.

    PubMed

    Garabito, M J; Arahal, D R; Mellado, E; Márquez, M C; Ventosa, A

    1997-07-01

    Bacillus salexigens sp. nov. is proposed based on the characteristics of six moderately halophilic, grampositive, rod-shaped strains isolated from salterns and hypersaline soils located in different geographical areas of Spain. These strains were motile, formed endospores, were strictly aerobic, were catalase and oxidase positive, and contained peptidoglycan of the meso-diamlnopimelic acid type in their vegetative cell walls. The DNA base compositions of these strains ranged from 36.3 to 39.5 mol%, and these organisms constitute a homology group with levels of DNA-DNA homology ranging from 73 to 100%. The 16S rRNA sequence of strain C-20MoT, which was used as the representative strain of these isolates, groups with the 16S rRNA sequences of members of the genus Bacillus, and the highest level of similarity is 95.4%. The type strain is strain C-20Mo (= ATCC 700290 = DSM 11483 = CCM 4646). PMID:9226905

  1. Nonomuraea thailandensis sp. nov. isolated from Thai soil.

    PubMed

    Sripreechasak, Paranee; Tanasupawat, Somboon; Suwanborirux, Khanit; Inahashi, Yuki; Matsumoto, Atsuko; Shiomi, Kazuro; Takahashi, Yoko

    2013-02-01

    A novel actinomycete, strain KC-061(T) was isolated from a soil sample collected from Angthong Islands National Park, Surat Thani province, Thailand. Strain KC-061(T) was identified as a member of the genus Nonomuraea through the use of a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequencing, strain KC-061(T) belongs to the genus Nonomuraea and was closely related to 'N. monospora' PT708(T) (99.3%), N. rhizophila YIM 67092(T) (98.6%), N. dietziae DSM 44320(T) (98.5%) and N. rosea GW 12687(T) (98.3%). On the basis of phenotypic characteristics, DNA-DNA relatedness and phylogenetic distinctiveness, the novel isolate was identified as representing a novel species of the genus Nonomuraea, for which the name Nonomuraea thailandensis sp. nov. (type strain KC-061(T) =JCM 18408(T)=KCTC 29074(T) =PCU 327(T)) is proposed. PMID:23188380

  2. Investigation of the biotransformation of TNT by a Rhodococcus sp.

    SciTech Connect

    Tharakan, J.P.; Welsh, G.; Johnson, J.H. Jr. [Howard Univ., Washington, DC (United States)

    1995-12-31

    Tri-nitro-toluene (TNT) has contaminated waterways and soils as a result of its widespread military and non-military uses. Several researches have isolated or constructed strains of microorganisms that are able to utilize TNT as sole carbon, nitrogen or energy sources. Researchers have also reported high TNT concentrations (> 50 mg/l) as inhibitory to bacteria, yeast and fungi. This study examines the degradation of TNT and pyrene using a Rhodococcus sp. isolated based on its ability to survive on pyrene as the sole carbon source. The experiments were designed to study both the direct and cometabolic transformation of TNT by the microbes in both batch and continuous modes, and to determine the optimum conditions under which TNT would be degradable.

  3. Failure Analysis of Nonvolatile Residue (NVR) Analyzer Model SP-1000

    NASA Technical Reports Server (NTRS)

    Potter, Joseph C.

    2011-01-01

    National Aeronautics and Space Administration (NASA) subcontractor Wiltech contacted the NASA Electrical Lab (NE-L) and requested a failure analysis of a Solvent Purity Meter; model SP-IOOO produced by the VerTis Instrument Company. The meter, used to measure the contaminate in a solvent to determine the relative contamination on spacecraft flight hardware and ground servicing equipment, had been inoperable and in storage for an unknown amount of time. NE-L was asked to troubleshoot the unit and make a determination on what may be required to make the unit operational. Through the use of general troubleshooting processes and the review of a unit in service at the time of analysis, the unit was found to be repairable but would need the replacement of multiple components.

  4. JSD: Parallel Job Accounting on the IBM SP2

    NASA Technical Reports Server (NTRS)

    Saphir, William; Jones, James Patton; Walter, Howard (Technical Monitor)

    1995-01-01

    The IBM SP2 is one of the most promising parallel computers for scientific supercomputing - it is fast and usually reliable. One of its biggest problems is a lack of robust and comprehensive system software. Among other things, this software allows a collection of Unix processes to be treated as a single parallel application. It does not, however, provide accounting for parallel jobs other than what is provided by AIX for the individual process components. Without parallel job accounting, it is not possible to monitor system use, measure the effectiveness of system administration strategies, or identify system bottlenecks. To address this problem, we have written jsd, a daemon that collects accounting data for parallel jobs. jsd records information in a format that is easily machine- and human-readable, allowing us to extract the most important accounting information with very little effort. jsd also notifies system administrators in certain cases of system failure.

  5. Study on bioactive compounds from Streptomyces sp. ANU 6277.

    PubMed

    Narayana, Kolla J P; Prabhakar, Peddikotla; Vijayalakshmi, Muvva; Venkateswarlu, Yenamandra; Krishna, Palakodety S J

    2008-01-01

    An attempt was made to study the bioactive compounds from a terrestrial Streptomyces sp. ANU 6277 isolated from laterite soil. Four active fractions were recovered from the solvent extracts obtained from the culture broth of five day-old strain. Three bioactive compounds were purified and identified as 3-phenylpropionic acid, anthracene-9,10-quinone and 8-hydroxyquinoline. The components of the partially purified fourth active fraction were analyzed by gas chromatography-mass spectrometry and identified as benzyl alcohol, phenylethyl alcohol and 2H-1, 4-benzoxazin-3 (4H)-one. Four active fractions were screened for antimicrobial activity against Gram-positive and Gram-negative bacteria, and fungi including phytopathogenic, toxigenic and dermatophytic genera. Among these metabolites, 8-hydroxyquinoline exhibited strong antibacterial and antifungal activity as compared to 3-phenylpropionic acid and anthracene-9,10-quinone. PMID:18610654

  6. Antifouling activities of marine bacteria associated with sponge ( Sigmadocia sp.)

    NASA Astrophysics Data System (ADS)

    Satheesh, S.; Soniamby, A. R.; Sunjaiy Shankar, C. V.; Mary Josephine Punitha, S.

    2012-09-01

    The present study aimed at assessing the antifouling activity of bacteria associated with marine sponges. A total of eight bacterial strains were isolated from the surface of sponge Sigmadocia sp., of them, SS02, SS05 and SS06 showed inhibitory activity against biofilm-forming bacteria. The extracts of these 3 strains considerably affected the extracellular polymeric substance producing ability and adhesion of biofilm-forming bacterial strains. In addition to disc diffusion assay, microalgal settlement assay was carried out with the extracts mixed with polyurethane wood polish and coated onto stainless steel coupons. The extract of strain SS05 showed strong microalgal settlement inhibitory activity. Strain SS05 was identified as Bacillus cereus based on its 16S rRNA gene. Metabolites of the bacterial strains associated with marine invertebrates promise to be developed into environment-friendly antifouling agents.

  7. Polypodium sp. (Coelenterata) infection of paddlefish (Polyodon spathula) eggs

    USGS Publications Warehouse

    Suppes, V. Charles; Meyer, Fred P.

    1975-01-01

    Parasitism of fish by coelenterates is rare and the first North American infection was reported in sturgeon (Hoffman et al., 1974, J Parasitol 60: 548-550). In the USSR parasitism of sturgeon (Acipenser sp.) eggs by the coelenterate Polypodium hydriforme Ussov 1885 has long been reported and investigated [Raikova, 1959, in Parasites of Freshwater Fish and the Biological Basis for their Control. Bull State Sci Res Inst Lake and River Fish. XLIX. Eng. transl. No. TT61-31056, O. T. S., U. S. Dep. Commerce, Washington, D. C., 1962, 235 p.: Raikova, 1973, (Proc Second Internat Symp on Cnidaria), Seto Marine Biological Laboratory 20: 165-173]. This report represents the first known infection of Polypodium in the paddlefish (Polyodon spathula).

  8. Immobilization of glucosyltransferase from Erwinia sp. using two different techniques.

    PubMed

    Contesini, Fabiano Jares; Ibarguren, Carolina; Grosso, Carlos Raimundo Ferreira; Carvalho, Patrícia de Oliveira; Sato, Hélia Harumi

    2012-04-15

    Two different techniques of glucosyltransferase immobilization were studied for the conversion of sucrose into isomaltulose. The optimum conditions for immobilization of Erwinia sp. glucosyltransferase onto Celite 545, determined using response surface methodology, was pH 4.0 and 170 U of glucosyltransferase/g of Celite 545. Using this conditions more than 60% conversion of sucrose into isomaltulose can be obtained. The immobilization of glucosyltransferase was also studied by its entrapment in microcapsules of low-methoxyl pectin and fat (butter and oleic acid). The non-lyophilized microcapsules of pectin, containing the enzyme and fat, showed higher glucosyltransferase activity, compared with lyophilized microcapsules containing enzyme plus fat, and also lyophilized microcapsules containing enzyme without fat addition. The non-lyophilized microcapsules of pectin containing the glucosyltransferase and fat, converted 30% of sucrose into isomaltulose in the first batch. However the conversion decreased to 5% at the 10th batch, indicating inactivation of the enzyme. PMID:22306307

  9. SP-100 fuel pin performance: Results from irradiation testing

    SciTech Connect

    Makenas, B.J.; Paxton, D.M. [Westinghouse Hanford Co., Richland, WA (United States); Vaidyanathan, S. [Martin Marietta, San Jose, CA (United States); Hoth, C.W. [Los Alamos National Lab., NM (United States)

    1993-09-01

    A total of 86 experimental fuel pins with various fuel, liner, and cladding candidate materials have been irradiated in the Experimental Breeder Reactor-II (EBR-II) and the Fast Flux Test Facility (FFTF) reactor as part of the SP-100 fuel pin irradiation testing program. Postirradiation examination results from these fuel pin are key in establishing performance correlations and demonstrating the lifetime and safety of the reactor fuel system. This paper provides a brief description of the in-reactor fuel pin tests and presents the most recent irradiation data on the performance of wrought rhenium (Re) liner material and high density UN fuel at goal burnup of 6 atom percent (at. %). It also provides an overview of the significant variety of other fuel/liner/cladding combinations which were irradiated as part of this program and which may be of interest to more advanced efforts.

  10. Orthorhombic C32: a novel superhard sp3 carbon allotrope.

    PubMed

    Zhang, Miao; Liu, Hanyu; Du, Yonghui; Zhang, Xinxin; Wang, Yanchao; Li, Quan

    2013-09-01

    Using a recently developed 'Crystal structure AnaLYsis by Particle Swarm Optimization' (CALYPSO) algorithm on a structural search, we predicted a novel sp(3) carbon allotrope possessing an orthorhombic lattice with the space group Cmmm (oC32). The calculated elastic constants and the simulated hardness revealed that oC32 simultaneously possesses ultra-incompressible and superhard properties with a high bulk modulus of 457 GPa and a high Vickers hardness of 96.2 GPa. This oC32 phase is dynamically stable and energetically more preferable than the experientially observed cold-compressed carbon, thus oC32 is expected to be experimentally synthesizable under extreme conditions. These results further expand the list of meta-stable carbon allotropes and superhard materials under atmospheric and extreme conditions. PMID:23872724

  11. The explicit representation for the angular flux solution in the simplified P{sub N} (SP{sub N}) theory

    SciTech Connect

    Chao, Y. A. [Apartment 101, Building 2, 788 Hong Xu Road, Shanghai 201103 (China); Yamamoto, A. [Nagoya Univ., Furo-cho, Chikusa-ku, Nagoya, 464-8603 (Japan)

    2012-07-01

    The current SP{sub n} theory formulation, via either the asymptotic method or the variational method, does not provide an explicit and calculable representation for the corresponding angular flux solution. It is therefore not possible to reconstruct from the SP{sub n} solution the corresponding angular flux solution, or to extract from a reference transport solution the corresponding SP{sub n} solution. This makes it impossible to calculate the necessary surface discontinuity factors to force consistency between the SP{sub n} solution and the higher level transport solution. Without discontinuity factors, the superiority of SP{sub n} over diffusion could be significantly degraded in practical applications. In this paper we present a different SP{sub n} formulation that provides the explicit angular flux solution such that the physical picture for the SP{sub n} approximation is transparent and the SP{sub n} discontinuity factors can be calculated. (authors)

  12. 78 FR 24803 - Hilco SP Rail, LLC-Acquisition and Operation Exemption-RG Steel Railroad Holding, LLC

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-26

    ...TRANSPORTATION Surface Transportation Board [Docket No. FD 35734] Hilco SP Rail, LLC--Acquisition and Operation Exemption--RG Steel Railroad Holding, LLC Hilco SP Rail, LLC (Hilco), a noncarrier, has filed a verified notice of...

  13. Genomic sequence analysis and characterization of Sneathia amnii sp. nov

    PubMed Central

    2012-01-01

    Background Bacteria of the genus Sneathia are emerging as potential pathogens of the female reproductive tract. Species of Sneathia, which were formerly grouped with Leptotrichia, can be part of the normal microbiota of the genitourinary tracts of men and women, but they are also associated with a variety of clinical conditions including bacterial vaginosis, preeclampsia, preterm labor, spontaneous abortion, post-partum bacteremia and other invasive infections. Sneathia species also exhibit a significant correlation with sexually transmitted diseases and cervical cancer. Because Sneathia species are fastidious and rarely cultured successfully in vitro; and the genomes of members of the genus had until now not been characterized, very little is known about the physiology or the virulence of these organisms. Results Here, we describe a novel species, Sneathia amnii sp. nov, which closely resembles bacteria previously designated "Leptotrichia amnionii". As part of the Vaginal Human Microbiome Project at VCU, a vaginal isolate of S. amnii sp. nov. was identified, successfully cultured and bacteriologically cloned. The biochemical characteristics and virulence properties of the organism were examined in vitro, and the genome of the organism was sequenced, annotated and analyzed. The analysis revealed a reduced circular genome of ~1.34 Mbp, containing ~1,282 protein-coding genes. Metabolic reconstruction of the bacterium reflected its biochemical phenotype, and several genes potentially associated with pathogenicity were identified. Conclusions Bacteria with complex growth requirements frequently remain poorly characterized and, as a consequence, their roles in health and disease are unclear. Elucidation of the physiology and identification of genes putatively involved in the metabolism and virulence of S. amnii may lead to a better understanding of the role of this potential pathogen in bacterial vaginosis, preterm birth, and other issues associated with vaginal and reproductive health. PMID:23281612

  14. Degradation of 3-phenylbutyric acid by Pseudomonas sp.

    PubMed Central

    Sariaslani, F S; Sudmeier, J L; Focht, D D

    1982-01-01

    Pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-PB) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. During early exponential growth, a catechol substance identified as 3-(2,3-dihydroxyphenyl)butyrate (2,3-DHPB) and its meta-cleavage product 2-hydroxy-7-methyl-6-oxononadioic-2,4-dienoic acid were produced. These products disappeared during late exponential growth, and considerable amounts of 2,3-DHPB reacted to form brownish polymeric substances. The catechol intermediate 2,3-DHPB could not be isolated, but cell-free extracts were able only to oxidize 3-(2,3-dihydroxyphenyl)propionate of all dihydroxy aromatic acids tested. Moreover, a reaction product caused by dehydration of 2,3-DHPB on silica gel was isolated and identified by spectral analysis as (--)-8-hydroxy-4-methyl-3,4-dihydrocoumarin. 3-Phenylpropionate and a hydroxycinnamate were found in supernatants of cultures grown on 3-PB; phenylacetate and benzoate were found in supernatants of cultures grown on 3-phenylpropionate; and phenylacetate was found in cultures grown on cinnamate. Cells grown on 3-PB rapidly oxidized 3-phenylpropionate, cinnamate, catechol, and 3-(2,3-dihydroxyphenyl)propionate, whereas 2-phenylpropionate, 2,3-dihydroxycinnamate, benzoate, phenylacetate, and salicylate were oxidized at much slower rates. Phenylsuccinate was not utilized for growth nor was it oxidized by washed cell suspensions grown on 3-PB. However, dual axenic cultures of Pseudomonas acidovorans and Klebsiella pneumoniae, which could not grow on phenylsuccinate alone, could grow syntrophically and produced the same metabolites found during catabolism of 3-PB by Pseudomonas sp. Washed cell suspensions of dual axenic cultures also immediately oxidized phenylsuccinate, 3-phenylpropionate, cinnamate, phenylacetate, and benzoate. PMID:7118830

  15. Properties of an amylase from thermophilic Bacillus SP

    PubMed Central

    de Carvalho, Raquel Vieira; Côrrea, Thamy Lívia Ribeiro; da Silva, Júlia Caroline Matos; de Oliveira Mansur, Luciana Ribeiro Coutinho; Martins, Meire Lelis Leal

    2008-01-01

    ?-Amylase production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing soluble starch as a carbon source and supplemented with 0.05% whey protein and 0.2% peptone reached a maximum activity at 32 h, with levels of 37 U/mL. Studies on the amylase characterization revealed that the optimum temperature of this enzyme was 90°C. The enzyme was stable for 1 h at temperatures ranging from 40-50°C while at 90°C, 66% of its maximum activity was lost. However, in the presence of 5 mM CaCl2, the enzyme was stable at 90°C for 30 min and retained about 58% residual activity after 1 h. The optimum pH of the enzyme was found to be 8.5. After incubation of enzyme for 2 h at pH 9.5 and 11.0 was observed a decrease of about 6.3% and 16.5% of its original activity. At pH 6.0 the enzyme lost about 36% of its original activity. The enzyme was strongly inhibited by Co2+, Cu2+ and Ba2+, but less affected by Mg2+, Na+ and K+. In the presence of 2.0 M NaCl, 63% of amylase activity was retained after 2 h incubation at 45°C. The amylase exhibited more than 70% activity when incubated for 1 h at 50°C with sodium dodecyl sulphate. However, very little residual activity was obtained with sodium hypochlorite and with hydrogen peroxide the enzyme was completely inhibited. The compatibility of Bacillus sp SMIA-2 amylase with certain commercial detergents was shown to be good as the enzyme retained 86%, 85% and 75% of its activity after 20 min incubation at 50°C in the presence of the detergent brands Omo®, Campeiro® and Tide®, respectively. PMID:24031188

  16. Generation of Enterobacter sp. YSU auxotrophs using transposon mutagenesis.

    PubMed

    Caguiat, Jonathan James

    2014-01-01

    Prototrophic bacteria grow on M-9 minimal salts medium supplemented with glucose (M-9 medium), which is used as a carbon and energy source. Auxotrophs can be generated using a transposome. The commercially available, Tn5-derived transposome used in this protocol consists of a linear segment of DNA containing an R6K? replication origin, a gene for kanamycin resistance and two mosaic sequence ends, which serve as transposase binding sites. The transposome, provided as a DNA/transposase protein complex, is introduced by electroporation into the prototrophic strain, Enterobacter sp. YSU, and randomly incorporates itself into this host's genome. Transformants are replica plated onto Luria-Bertani agar plates containing kanamycin, (LB-kan) and onto M-9 medium agar plates containing kanamycin (M-9-kan). The transformants that grow on LB-kan plates but not on M-9-kan plates are considered to be auxotrophs. Purified genomic DNA from an auxotroph is partially digested, ligated and transformed into a pir+ Escherichia coli (E. coli) strain. The R6K? replication origin allows the plasmid to replicate in pir+ E. coli strains, and the kanamycin resistance marker allows for plasmid selection. Each transformant possesses a new plasmid containing the transposon flanked by the interrupted chromosomal region. Sanger sequencing and the Basic Local Alignment Search Tool (BLAST) suggest a putative identity of the interrupted gene. There are three advantages to using this transposome mutagenesis strategy. First, it does not rely on the expression of a transposase gene by the host. Second, the transposome is introduced into the target host by electroporation, rather than by conjugation or by transduction and therefore is more efficient. Third, the R6K? replication origin makes it easy to identify the mutated gene which is partially recovered in a recombinant plasmid. This technique can be used to investigate the genes involved in other characteristics of Enterobacter sp. YSU or of a wider variety of bacterial strains. PMID:25408097

  17. A novel gene encoding a 54 kDa polypeptide is essential for butane utilization by Pseudomonas sp. IMT37

    Microsoft Academic Search

    R. S. Padda; K. K. Pandey; S. Kaul; V. D. Nair; R. K. Jain; S. K. Basus; T. Chakrabarti

    Twenty-three propane- and butane-utilizing bacteria were isolated from soil samples collected from oilfields. Three of them have been identified as Rhodococcus sp. IMT35, Pseudomonas sp. IMT37 and Pseudomonas sp. IMT40. SDS-PAGE analysis of the membrane of Rhodococcus sp. IMT35 revealed the presence of at least four polypeptides induced by propane. Polyclonal antibody raised against a 58 kDa polypeptide from Rhodococcus

  18. Two new species of Pharta Thorell, 1891 with the description of Ibana senagang gen. et sp. nov. (Araneae: Thomisidae).

    PubMed

    Benjamin, Suresh P

    2014-01-01

    Two new species of Pharta, P. sudmannorum sp. nov. (??, Borneo) and P. koponeni sp. nov. (?, Thailand) are described. Furthermore, Ibana senagang gen. nov. & sp. nov. from Malaysia is described based on its exceptional palp, which has a reduced, movable conductor and thick-long spines on the distal, ventral surface of the tibia, reminiscent of Epidius Thorell, 1877. PMID:25544630

  19. OfOLITH ULTRASTRUcrURE OF SMOOTH OREO, PSEUDOCYTTUS MACULATUS, AND BLACK OREO, ALWCYTTUS SP., SPECIES

    E-print Network

    OfOLITH ULTRASTRUcrURE OF SMOOTH OREO, PSEUDOCYTTUS MACULATUS, AND BLACK OREO, ALWCYTTUS SP'Udocyttus maculat"U8 and Allocyttus sp. are not suitable for age estimation. The smooth oreo, Pseudocyttus maculatus, and the black oreo, Allocyttus sp., are two related species of the family Oreosomatidae

  20. Genome Sequence of the Halotolerant Staphylococcus sp. Strain OJ82, Isolated from Korean Traditional Salt-Fermented Seafood

    PubMed Central

    Sung, Jung-Suk; Chun, Jongsik; Choi, Sungjong

    2012-01-01

    Staphylococcus sp. strain OJ82 was isolated from a Korean traditional fermented squid seafood, ojingeo-jeotgal. Staphylococcus sp. OJ82 could grow and show extracellular protease and ?-galactosidase activities in the presence of extremely high saline (20%). Here, we report the genome sequence of Staphylococcus sp. OJ82. PMID:23105083

  1. Genome sequence of the halotolerant Staphylococcus sp. strain OJ82, isolated from Korean traditional salt-fermented seafood.

    PubMed

    Sung, Jung-Suk; Chun, Jongsik; Choi, Sungjong; Park, Woojun

    2012-11-01

    Staphylococcus sp. strain OJ82 was isolated from a Korean traditional fermented squid seafood, ojingeo-jeotgal. Staphylococcus sp. OJ82 could grow and show extracellular protease and ?-galactosidase activities in the presence of extremely high saline (20%). Here, we report the genome sequence of Staphylococcus sp. OJ82. PMID:23105083

  2. Biotransformation pathways of ginsenoside Rb1 to compound K by ?-glucosidases in fungus Paecilomyces Bainier sp. 229

    Microsoft Academic Search

    Qin Yan; Wei Zhou; XunLong Shi; Pei Zhou; DianWen Ju; MeiQing Feng

    2010-01-01

    Ginsenoside Rb1 is the most abundant ginsenoside in Panax (ginseng). The hydrolysis of this ginsenoside produces compound K, the biologically active ginsenoside of ginseng. We previously identified a fungus Paecilomyces Bainier sp. 229 (sp. 229), which can efficiently convert ginsenoside Rb1 to compound K. In this report, the ginsenoside hydrolyzing ?-glucosidases were isolated from sp. 229 and the pathway of

  3. 77 FR 71480 - Proposed Collection; Comment Request for Form 4506T-EZ, 4506-T-EZ (SP)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-30

    ...Request for Form 4506T-EZ, 4506-T-EZ (SP) AGENCY: Internal Revenue Service (IRS...Tax Return Transcript, and 4506T-EZ (SP), Formulario Abreviado para la Solicitud...Declaracion de Impuestos Personales (4506T-EZ (SP)). OMB Number: 1545-2154. Form...

  4. The antagonistic action of Trichoderma sp. hyphae to Lentinula edodes hyphae changes lignocellulotytic activities during cultivation in wheat straw

    Microsoft Academic Search

    J.-M. Savoie; G. Mata

    1999-01-01

    Lentinula edodes (Berk.) Pegler was cultivated in sterilized or pasteurized wheat straw both with and without inoculation with Trichoderma sp. Enhancements of ß-mannosidase and laccase activities and lowering of Mn-dependent peroxidase activity were observed seven days after inoculation in substrates inoculated with Trichoderma sp. These enzymes were not produced by Trichoderma sp. Most of the polysaccharidase activities were higher in

  5. Polymerized sp2-sp3 hybrid metallic phase of C60 as obtained via constant-pressure molecular dynamics

    NASA Astrophysics Data System (ADS)

    Yamagami, Yuichiro; Saito, Susumu

    2009-01-01

    Using the tight-binding molecular-dynamics method, we study the pressure-induced structural phase transition of the two-dimensional “tetragonal” C60 polymer. It is found that the structural transition to a three-dimensionally (3D) polymerized C60 phase takes place for a wide range of pressure from 12 to 28 GPa. Although the lattice constants of this 3D polymer phase are close to those of experimentally observed values [S. Yamanaka , Phys. Rev. Lett. 96, 076602 (2006)], the present phase has much more sp3 C sites than the experimentally proposed phase and each C60 has interfullerene chemical bonds with all the 12 neighbor C60 fullerenes. From the subsequent total-energy electronic-structure analysis in the framework of the density-functional theory, it is found that this completely polymerized C60 phase has much lower total energy than the previously proposed 3D polymer phases and possesses the metallic electronic structure.

  6. Kinetics of the conversion of broken diamond (sp{sup 3}) bonds to graphitic (sp{sup 2}) bonds

    SciTech Connect

    Reznik, A.; Richter, V.; Kalish, R. [Physics Department and Solid State Institute, Technion-Israel Institute of Technology, Haifa, 32000 (Israel)] [Physics Department and Solid State Institute, Technion-Israel Institute of Technology, Haifa, 32000 (Israel)

    1997-10-01

    The thermally driven structural modification of diamond damaged by low-temperature Xe ion implantation are studied by following the changes in its electrical resistivity as function of annealing temperature and time while it gradually converts to graphite. The conduction mechanism in damaged and in partially graphitized diamond is well described by the variable-range-hopping mechanism, allowing the extraction of a number of hopping sites at different stages of annealing from the temperature dependence of the resistivity. It is found that conversion of broken, metastable diamond bonds to graphitic bonds sets in already at an annealing temperature of 420 K with an activation energy of about 0.7 eV, thus giving an estimate of the height of the barrier which separates broken sp{sup 3} bonds from graphitic bonds. {copyright} {ital 1997} {ital The American Physical Society}

  7. Structural determination of hypnosin, a spore germination inhibitor of phytopathogenic Streptomyces sp. causing root tumor in melon (Cucumis sp.).

    PubMed

    Aoki, Yuu; Yoshida, Masahiro; Kawaide, Hiroshi; Abe, Hiroshi; Natsume, Masahiro

    2007-12-26

    The structure of a germination inhibitor, hypnosin, isolated from phytopathogenic Streptomyces sp. causing root tumor of melon was determined to be 3-acetylaminopyrazine-2-carboxylic acid (1) by mass spectrometry, computational chemical prediction of UV spectrum, and synthesis of candidates. The structure-activity relationship of hypnosin and anthranilic acid was examined, and it was concluded that pyrazinecarboxylic acid or pyridine-2-carboxylic acid was the fundamental structure with activity, that methylation of the carboxyl group or decarboxylation destroyed activity, and that the presence of an amino group was inhibitory to the activity, whereas acetylation or deletion of an amino group enhanced activity. Hypnosin inhibited spore germination of some Streptomyces spp. in addition to the species with which it was isolated. PMID:18052243

  8. Neptunomonas phycophila sp. nov. isolated from a culture of Symbiodinium sp., a dinoflagellate symbiont of the sea anemone Aiptasia tagetes.

    PubMed

    Frommlet, Jörg; Guimarães, Bárbara; Sousa, Lígia; Serôdio, João; Alves, Artur

    2015-03-01

    A Gram-stain-negative, facultatively anaerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain SYM1(T), was isolated from a culture of Symbiodinium sp., an algal symbiont of the sea anemone Aiptasia tagetes collected in Puerto Rico. Growth was observed at 4-40 °C (optimum 30 °C), at pH 5.0-11.0 (optimum pH 8.0) and with 0.5-8?% (optimum 2?%) (w/v) NaCl. Phylogenetic analyses of 16S rRNA gene sequences showed that strain SYM1(T) was a member of the genus Neptunomonas with the type strain of Neptunomonas naphthovorans as the closest phylogenetic relative with a pairwise sequence similarity of 98.15?%. However, DNA-DNA relatedness between strain SYM1(T) and N. naphthovorans CIP 106451(T) was 24?%. Moreover, strain SYM1(T) could be distinguished from its closest relative by several phenotypic characteristics such as NaCl, pH and temperature tolerance, nitrate reduction and utilization of carbon substrates. The major cellular fatty acids were C16?:?0, C18?:?1?7c and summed feature 3 (comprising C16?:?1?7c and/or iso-C15?:?0 2-OH). The genomic DNA G+C content of strain SYM1(T) was 45 mol%. Ubiquinone-8 (Q-8) was the only respiratory quinone detected. Based on a polyphasic taxonomic characterization, strain SYM1(T) represents a novel species of the genus Neptunomonas, for which the name Neptunomonas phycophila sp. nov. is proposed. The type strain is SYM1(T) (?=?LMG 28329(T)?=?CECT 8716(T)). PMID:25563909

  9. Yarrowia porcina sp. nov. and Yarrowia bubula f.a. sp. nov., two yeast species from meat and river sediment.

    PubMed

    Nagy, Edina; Dlauchy, Dénes; Medeiros, Adriana O; Péter, Gábor; Rosa, Carlos A

    2014-04-01

    Eleven yeast strains representing two hitherto undescribed species were isolated from different kinds of meat samples in Hungary and one from the sediment of a tropical freshwater river in Southeastern Brazil. The analysis of the sequences of their large subunit rRNA gene D1/D2 domain and the internal transcribed spacer (ITS) regions placed the two new species in the Yarrowia clade. Some of the seven strains representing the first new species can mate and give rise to asci and form ascospores embedded in capsular material, which qualifies it as the third teleomorph species of the Yarrowia clade. The name Yarrowia porcina sp. nov. (type strain: NCAIM Y.02100(T) = CBS 12935(T) = NRRL Y-63669(T), allotype strain UFMG-RD131(A) = CBS 12932(A)) is proposed for this new yeast species, which, based on physiological characters, is indistinguishable from Yarrowia lipolytica and some other species of the genus. Considerable intraspecific variability was detected among the sequences of the large subunit rRNA gene D1/D2 domains of the seven strains. The variability among the D1/D2 sequences exceeded the divergence observed among the ITS sequences and in some cases more than 1 % substitution among the D1/D2 sequences was detected. The conspecificity of these strains was supported by the low (0-3 substitutions) sequence divergence among their ITS sequences, the result of a parsimony network analysis utilizing the concatenated ITS and D1/D2 sequences and also by the fingerprint patterns generated by microsatellite primed PCR. No ascospore formation was observed in the group of the other five strains representing the second new species. These strains shared identical D1/D2 and ITS sequences. Yarrowia bubula f.a., sp. nov. (type strain: NCAIM Y.01998(T) = CBS 12934(T) = NRRL Y-63668(T)) is proposed to accommodate these strains. PMID:24500004

  10. Jeotgalicoccus halotolerans gen. nov., sp. nov. and Jeotgalicoccus psychrophilus sp. nov., isolated from the traditional Korean fermented seafood jeotgal.

    PubMed

    Yoon, Jung-Hoon; Lee, Keun-Chul; Weiss, Norbert; Kang, Kook Hee; Park, Yong-Ha

    2003-03-01

    Two Gram-positive, non-motile, non-spore-forming, halotolerant and moderately halophilic cocci (strains YKJ-101T and YKJ-115T) were isolated from the traditional Korean fermented seafood jeotgal, and were investigated using a polyphasic taxonomic approach. Phylogenetic analysis of 16S rDNA sequences showed that strains YKJ-101T and YKJ-115T are most closely related to the cluster comprising two Salinicoccus species. The peptidoglycan type of the strains is A3alpha, based on L-Lys-Gly(3-4)-L-Ala(Gly), and the predominant menaquinone is MK-7. Strains YKJ-101T and YKJ-115T have cellular fatty acid profiles containing major amounts of saturated, unsaturated and branched fatty acids; the major fatty acids are anteiso-C15 : 0 and iso-C15 : 0. The cellular polar lipids are phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipids. Strains YKJ-101T and YKJ-115T have identical DNA G + C contents of 42 mol%. The 16S rDNA similarity between strains YKJ-101T and YKJ-115T is 98% and the mean level of DNA-DNA relatedness between the two strains is 13.4%. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, it is proposed that strains YKJ-101T and YKJ-115T should be placed in a new genus, Jeotgalicoccus gen. nov., as two distinct new species, for which the names Jeotgalicoccus halotolerans sp. nov. and Jeotgalicoccus psychrophilus sp. nov. are proposed. The type strains are YKJ-101T (=KCCM 41448T =JCM 11198T) and YKJ-115T (=KCCM 41449T =JCM 11199T), respectively. PMID:12710632

  11. Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China.

    PubMed

    Gao, Jun-Lian; Turner, Sarah Lea; Kan, Feng Ling; Wang, En Tao; Tan, Zhi Yuan; Qiu, Yu Hui; Gu, Jun; Terefework, Zewdu; Young, J Peter W; Lindström, Kristina; Chen, Wen Xin

    2004-11-01

    Ninety-five rhizobial strains isolated from Astragalus adsurgens growing in the northern regions of China were classified into three main groups, candidate species I, II and III, based on a polyphasic approach. Comparative analysis of full-length 16S rRNA gene sequences of representative strains showed that candidate species I and II were Mesorhizobium, while candidate species III, which consisted of non-nodulating strains, was closely related to Agrobacterium tumefaciens. The phylogenetic relationships of the three candidate species and some related strains were also confirmed by the sequencing of glnA genes, which were used as an alternative chromosomal marker. The DNA-DNA relatedness was between 11.3 and 47.1 % among representative strains of candidate species I and II and the type strains of defined Mesorhizobium species. Candidate III had DNA relatedness of between 4.3 and 25.2 % with type strains of Agrobacterium tumefaciens and Agrobacterium rubi. Two novel species are proposed to accommodate candidate species I and II, Mesorhizobium septentrionale sp. nov. (type strain, SDW014(T)=CCBAU 11014(T)=HAMBI 2582(T)) and Mesorhizobium temperatum sp. nov. (type strain, SDW018(T)=CCBAU 11018(T)=HAMBI 2583(T)), respectively. At least two distinct nodA sequences were identified among the strains. The numerically dominant nodA sequence type was most similar to that from the Mesorhizobium tianshanense type strain and was identified in strains belonging to the two novel species as well as other, as yet, undefined genome types. Host range studies indicate that the different nodA sequences correlate with different host ranges. Further comparative studies with the defined Agrobacterium species are needed to clarify the taxonomic identity of candidate species III. PMID:15545425

  12. Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese.

    PubMed

    Brennan, N M; Brown, R; Goodfellow, M; Ward, A C; Beresford, T P; Simpson, P J; Fox, P F; Cogan, T M

    2001-05-01

    Ten isolates each of two different bacterial species isolated from the surface of a smear-ripened cheese were found to exhibit many characteristics of the genus Corynebacterium. The isolates were Gram-positive, catalase-positive, non-spore-forming rods that did not undergo a rod/coccus transformation when grown on complex media. Chemotaxonomic investigation revealed that the strains belonged unambiguously to the genus Corynebacterium. Their cell walls contained arabinose, galactose and short-chain mycolic acids (C22 to C36) and their peptidoglycan contained meso-diaminopimelic acid. The G+C content of the DNA was 51-60 mol%. MK-9 (H2) was the principal menaquinone. The 16S rDNA sequences of four isolates of each bacterium were determined and aligned with those of other members of the coryneform group. Phylogenetic analysis showed that the strains represented two new sublines within the genus Corynebacterium; Corynebacterium variabile and Corynebacterium ammoniagenes were their nearest known phylogenetic neighbours. Corynebacterium variabile and Corynebacterium ammoniagenes showed the highest levels of sequence homology with the isolates; however, DNA-DNA hydridization studies indicated that the Corynebacterium strains isolated from the cheese smear did not belong to either Corynebacterium variabile or Corynebacterium ammoniagenes (26 and 46% chromosomal similarity, respectively). On the basis of the phylogenetic and phenotypic distinctiveness of the unknown isolates, it is proposed that the bacteria be classified as two new Corynebacterium species, for which the names Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov. are proposed. Type strains have been deposited in culture collections as Corynebacterium mooreparkense LMG S-19265T (= NCIMB 30131T) and Corynebacterium casei LMG S-19264T (= NCIMB 30130T). PMID:11411705

  13. Sphingobacterium lactis sp. nov. and Sphingobacterium alimentarium sp. nov., isolated from raw milk and a dairy environment.

    PubMed

    Schmidt, Verena S J; Wenning, Mareike; Scherer, Siegfried

    2012-07-01

    Four non-fermenting, rod-shaped, Gram-staining-negative bacterial strains, designated WCC 4512(T), WS 4555, WCC 4521(T) and WS 4556, were isolated from raw milk and the dairy environment. Phylogenetic analyses based on 16S rRNA and groEL gene sequences demonstrated the affiliation of the four strains to two distinct clusters within the class Sphingobacteriia, phylum 'Bacteroidetes'. Strains WCC 4512(T) and WS 4555 showed the highest 16S rRNA gene sequence similarity to the type strain of S. daejeonense (97.3 and 97.2%, respectively), whereas strains WCC 4521(T) and WS 4556 were most closely related to S. composti LMG 23401(T) (97.6% 16S rRNA gene sequence similarity). The DNA G+C contents of strains WCC 4512(T) and WCC 4521(T) were 44.2 and 39.3 mol%, respectively. The major cellular fatty acids and the presence of menaquinone MK-7 as the predominant quinone for both strains WCC 4512(T) and WCC 4521(T) supported their affiliation to the genus Sphingobacterium. DNA-DNA hybridization experiments between strain WCC 4512(T) and S. daejeonense LMG 23402(T) and between strain WCC 4521(T) and S. composti LMG 23401(T) revealed DNA relatedness values of 2% (repetition, 3%) and 8% (repetition, 17%), respectively. On the basis of phenotypic and genetic properties, as well as phylogenetic distinctiveness, it is suggested that the four strains represent two novel Sphingobacterium species with strain WCC 4512(T) (=DSM 22361(T)=LMG 25272(T)) as the type strain of Sphingobacterium lactis sp. nov. (WS 4555 is a reference strain of S. lactis) and strain WCC 4521(T) (=DSM 22362(T)=LMG 25273(T)) as the type strain of Sphingobacterium alimentarium sp. nov. (WS 4556 is a reference strain of S. alimentarium). PMID:21856986

  14. New Species of Boletellus Section Boletellus (Boletaceae, Boletales) from Japan, B. aurocontextus sp. nov. and B. areolatus sp. nov.

    PubMed Central

    Sato, Hirotoshi; Hattori, Tsutomu

    2015-01-01

    We describe and illustrate two new species of Boletellus section Boletellus, B. aurocontextus sp. nov. and B. areolatus sp. nov., which are generally assumed to be B. emodensis. In this study, we reconstructed separate molecular phylogenetic trees of section Boletellus using the nucleotide sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA, the largest subunit (RPB1) and the second-largest subunit (RPB2) of nuclear RNA polymerase II gene and mitochondrial cytochrome oxidase subunit 3 (cox3) gene. We also examined the morphologies of B. emodensis sensu lato (s.l.) and other related species for comparison. The molecular phylogenetic tree inferred from the sequences of nuclear DNA (ITS, and combined dataset of RPB1 and RPB2) indicated that three genetically and phylogenetically well-separated lineages were present within B. emodensis s.l. These three lineages were also distinguished on the basis of the molecular phylogenetic tree constructed using the sequences of mitochondrial DNA (cox3), suggesting distinct cytonuclear disequilibria (i.e., evidence of reproductive isolation) among these lineages. Therefore, these three lineages can be treated as independent species: B. aurocontextus, B. areolatus, and B. emodensis. Boletellus aurocontextus and B. areolatus are also distinct from B. emodensis by the macro- and microscopic morphologies. Boletellus aurocontextus is characterized by a pileus with bright yellow to lemon yellow context, which can be observed through a gap in the scales, and basidiospores with relatively large length (mean spore length, 21.4 ?m; quotient of spore length and width, 2.51). In contrast, B. areolatus is characterized by a pileus with floccose to appressed thin scaly patches, a stipe with pallid or pale cream color at the upper half, and basidiospores with relatively small length (mean spore length, 16.5 ?m; quotient of spore length and width, 1.80). PMID:26083243

  15. Streptomyces chiangmaiensis sp. nov. and Streptomyces lannensis sp. nov., isolated from the South-East Asian stingless bee (Tetragonilla collina).

    PubMed

    Promnuan, Yaowanoot; Kudo, Takuji; Ohkuma, Moriya; Chantawannakul, Panuwan

    2013-05-01

    Two novel actinomycetes, strains TA4-1(T) and TA4-8(T,) were isolated from the South-East Asian stingless bee (Tetragonilla collina Smith 1857), collected from Chiang Mai Province, Thailand. The morphological and chemotaxonomic properties of strains TA4-1(T) and TA4-8(T) were consistent with the genus Streptomyces, i.e. the formation of aerial mycelia bearing spiral spore chains, the presence of the ll-isomer of diaminopimelic acid in cell walls, iso- and anteiso-branched fatty acids with carbon chain lengths 14-17 atoms as the major fatty acids and MK-9(H8) as the predominant menaquinone plus minor amounts of MK-9(H6) and MK-9(H10). Analysis of 16S rRNA gene sequences showed that strains TA4-1(T) and TA4-8(T) exhibited 98.8 and 98.1% sequence similarity, respectively, with Streptomyces chromofuscus NRRL B-12175(T) and 98.9% sequence similarity with each other. This study suggested that strains TA4-1(T) and TA4-8(T) were distinct from previously described species of the genus Streptomyces. In addition, the low degrees of DNA-DNA relatedness between the isolates and S. chromofuscus JCM 4354(T) warranted assigning strains TA4-1(T) and TA4-8(T) to two novel species. The names Streptomyces chiangmaiensis sp. nov. (type strain TA4-1(T) ?=?JCM 16577(T) ?=?TISTR 1981(T)) and Streptomyces lannensis sp. nov. (type strain TA4-8(T) ?=?JCM 16578(T) ?=?TISTR 1982(T)) are proposed. The species names indicate the geographical locations where the stingless bees reside. PMID:23002051

  16. Sphingomonas rosea sp. nov. and Sphingomonas swuensis sp. nov., rosy colored ?-glucosidase-producing bacteria isolated from soil.

    PubMed

    Srinivasan, Sathiyaraj; Lee, Jae-Jin; Kim, Myung Kyum

    2011-08-01

    Two strains PB196(T) and PB62(T) of Gram-negative, non-motile, and non-spore-forming bacteria, were isolated from soil in South Korea and characterized to determine their taxonomic positions. 16S rRNA gene sequence analysis showed that the two strains belonged to the genus Sphingomonas. The highest degree of sequence similarity of strain PB196(T) was found with PB62(T) (98.9%), Sphingomonas humi PB323(T) (98.9%), Sphingomonas kaistensis PB56(T) (98.2%), and Sphingomonas astaxanthinifaciens TDMA-17(T) (98.0%). The highest degree of sequence similarity of strain PB62(T) was found with Sphingomonas humi PB323(T) (98.8%), Sphingomonas astaxanthinifaciens TDMA-17(T) (98.2%), and Sphingomonas kaistensis PB56(T) (98.1%). Chemotaxonomic data revealed that they possessed ubiquinone-10 (Q-10) as common in the genus Sphingomonas, that the predominant fatty acids were summed feature 7 (C(18:1) ?7c/?9t/?12t), summed feature 4 (C(16:1) ?7c/C(15:0) iso 2OH), C(16:0), and C(17:1) ?6c, and that they contained sphingoglycolipid, phosphatidylglycerol (PG), and phosphatidyle-thanolamine (PE) in common but they showed difference for diphosphatidylglycerol (DPG). Based on these data, PB196(T) (=KCTC 12339(T) =JCM 16604(T)) and PB62(T) (=KCTC 12336(T) =JCM 16605(T) =KEMB 9004-005(T)) should be classified as type strains of two novel species, for which the names Sphingomonas rosea sp. nov. and Sphingomonas swuensis sp. nov. are proposed, respectively. PMID:21887644

  17. Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects.

    PubMed

    Fang, Baozhu; Liu, Chongxi; Guan, Xuejiao; Song, Jia; Zhao, Junwei; Liu, Hui; Li, Chuang; Ning, Wenxi; Wang, Xiangjing; Xiang, Wensheng

    2015-07-01

    Two novel actinobacteria, strains NEAU-CX1(T) and NEAU-JC6(T), were isolated from nymphs of stinkbug (Palomena viridissima Poda) and a beetle (Harpalus sinicus Hope), respectively, collected from Harbin, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-CX1(T) showed it to be most closely related to Micromonospora coxensis JCM 13248(T) (99.3 % sequence similarity), Micromonospora purpureochromogenes DSM 43821(T) (99.1 %) and Micromonospora halophytica JCM 3125(T) (98.6 %), and that of strain NEAU-JC6(T) to Micromonospora haikouensis DSM 45626(T) (99.3 %), Micromonospora carbonacea JCM 3139(T) (99.1 %) and Micromonospora krabiensis JCM 12869(T) (99.1 %). The phylogenetic analysis based on gyrB gene sequence of strain NEAU-CX1(T) showed it to be most closely related to M. purpureochromogenes DSM 43821(T) (98.0 % sequence similarity), and that of strain NEAU-JC6(T) to M. haikouensis DSM 45626(T) (99.0 %) and M. carbonacea JCM 3139(T) (98.0 %). A combination of DNA-DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Thus, strains NEAU-CX1(T) and NEAU-JC6(T) represent two novel species of the genus Micromonospora, for which the names Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. are proposed. The type strains are NEAU-CX1(T) (=CGMCC 4.7175(T) = JCM 30056(T)) and NEAU-JC6(T) (=CGMCC 4.7173(T) = JCM 30055(T)). PMID:25957972

  18. The Effect of Desulfovibrio sp. Biofilms on Corrosion Behavior of Copper in Sulfide-Containing Solutions

    NASA Astrophysics Data System (ADS)

    Güngör, Nihal Do?ruöz; Çotuk, Ay??n; D??p?nar, Derya

    2015-03-01

    This study aims to detect the effect of Desulfovibrio sp. on copper in terms of biofilm formation and corrosion in 722 h. In that way, appropriate strategies to inhibit microbiological corrosion in copper systems with Desulfovibrio sp. can be evaluated. For this purpose, experiments were performed in 1 L glass model system containing 28 copper coupons and pure culture of the sulfate-reducing bacteria (SRB) strain Desulfovibrio sp. in Postgate's medium C. Also, a control system with copper coupons but without Desulfovibrio sp. containing sterile Postgate's medium was studied concurrently with the test system. The test coupons were collected from systems at certain time intervals, namely 24, 168, 360, and 720 h. The samples were then subjected to several characterization analyses such as measurement of Desulfovibrio sp. numbers, corrosion resistance, EPS extraction, carbohydrate analysis, SEM, and EDS. During the experiments, the maximum Desulfovibrio sp. count in biofilm samples was found at 360 h. Carbohydrate and copper concentrations in biofilm were increased over time. EDS analysis revealed Cu, S, C, O, and Cl peaks on the surface of the samples. For the control coupons, only Cu peaks were observed. The results obtained from this study showed that copper was corroded by Desulfovibrio sp. in the model system under laboratory conditions.

  19. Genetic Variation in SP-A2 Leads to Differential Binding to Mycoplasma pneumoniae Membranes and Regulation of Host Responses.

    PubMed

    Ledford, Julie G; Voelker, Dennis R; Addison, Kenneth J; Wang, Ying; Nikam, Vinayak S; Degan, Simone; Kandasamy, Pitachaimani; Tanyaratsrisakul, Sasipa; Fischer, Bernard M; Kraft, Monica; Hollingsworth, John W

    2015-06-15

    Mycoplasma pneumoniae is an extracellular pathogen that colonizes mucosal surfaces of the respiratory tract and is associated with asthma exacerbations. Previous reports demonstrate that surfactant protein-A (SP-A) binds live M. pneumoniae and mycoplasma membrane fractions (MMF) with high affinity. Humans express a repertoire of single-amino acid genetic variants of SP-A that may be associated with lung disease, and our findings demonstrate that allelic differences in SP-A2 (Gln223Lys) affect the binding to MMF. We show that SP-A(-/-) mice are more susceptible to MMF exposure and have significant increases in mucin production and neutrophil recruitment. Novel humanized SP-A2-transgenic mice harboring the hSP-A2 223K allele exhibit reduced neutrophil influx and mucin production in the lungs when challenged with MMF compared with SP-A(-/-) mice. Conversely, mice expressing hSP-A2 223Q have increased neutrophil influx and mucin production that are similar to SP-A(-/-) mice. Using tracheal epithelial cell cultures, we show that enhanced mucin production to MMF occurs in the absence of SP-A and is not dependent upon neutrophil recruitment. Increased phosphorylation of the epidermal growth factor receptor (EGFR) was evident in the lungs of MMF-challenged mice when SP-A was absent. Pharmacologic inhibition of EGFR prior to MMF challenge dramatically reduced mucin production in SP-A(-/-) mice. These findings suggest a protective role for SP-A in limiting MMF-stimulated mucin production that occurs through interference with EGFR-mediated signaling. SP-A interaction with the EGFR signaling pathway appears to occur in an allele-specific manner that may have important implications for SP-A polymorphisms in human diseases. PMID:25957169

  20. Freshwater abiotic components' impact on the viability of fish lice, Argulus sp., in Guangdong province, China.

    PubMed

    Alsarakibi, Muhamd; Wadeh, Hicham; Li, Guoqing

    2012-07-01

    Parasite intensity dynamics can be strongly affected by ecosystem abiotic components. We conducted this study to investigate how the intensity of Argulus sp. is influenced by physicochemical factors in three water bodies - river, fish farm, and reservoir - and to find the occurrence of Argulus sp. under different conditions. Argulus sp. specimens were collected using different methods - gill-netting 30 × 1.5 m, 40 mm mesh monofilament net and set from a small boat, beach seining and plankton net - from July 2010 until Jun 2011 at Guangdong province, China. Main water parameters taken for the assessment consisting of temperature, pH, DO, biochemical oxygen demand of 5 days (BOD5), chemical oxygen demand (COD), NH (4) (+) , total nitrogen (TN) and total phosphorus (TP). The results show that the ratio of female to male Argulus sp. during the studied period was 2.3:1. The presence and intensity of Argulus sp. were temperature-dependent and variable; pH values were lower than standers with high intensity of Argulus sp. Argulus sp. has the ability to live in low dissolved oxygen (DO) concentration. Relatively high concentrations of BOD5, COD, NH (4) (+) , TN and TP were detected with related variable intensity of Argulus sp., which is supposed to be a freshwater fish ectoparasite found in polluted ecosystems. It is concluded that the fish ectoparasite Argulus sp. have a strong potency of genetic adaptation impacted by environmental factors. However, this needs further study in order to gain insight into the question of the likelihood of adaptation to abiotic factors' variation. PMID:22322389