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1

¿Se ha desarrollado el mercado secundario de acciones colombiano durante el período 1988-2002?  

Microsoft Academic Search

Este trabajo busca analizar el desarrollo del mercado secundario de acciones para Colombia desde 1988 hasta 2002. Para ello se toman cuatro variables: tamaño, liquidez, concentración e integración internacional. Esta investigación complementa trabajos anteriores sobre el mercado de acciones, como el de Demirgüç-Kunt y Levine (1995), a nivel internacional, y el de Arbeláez, Zuluaga y Guerra (2002) entre otros, a

Humberto Bernal Castro; ByronOrtega Gaitán

2004-01-01

2

SP Fonts  

NSDL National Science Digital Library

If you're looking for funky fonts to include in Web designs, papers, or posters, SP Fonts may be your answer. Scholars Press (SP) Fonts "are a set of simple, public domain fonts" designed for print and non-commercial Web use. This site currently offers eight public domain fonts that may be downloaded and used free of charge. Three of the fonts are Hebrew/Aramaic fonts: "SPTiberian (a standard Hebrew font), SPDamascus (a thinner font with Palestinian as well as Tiberian vowel points), and SPEzra (a simple, fixed-width Hebrew font)." Two are Greek fonts: "SPIonic (a more complete Greek font) and SPDoric (a simpler, uncial font)." Other fonts include "SPEdessa (a Syriac Estrangela font), SPAchmim (a Coptic font), and SPAtlantis (a transliteration font that includes diacriticals and other special characters that allow the representation of numerous Indo-European, Semitic, and other languages"-- available in both Roman and Italic type). All fonts are TrueType fonts and are compatible with PC (Windows) and Mac computers. In addition, each font has a .readme file that explains the standard keyboard mapping used by the font. Although the fonts are free to the public, the Web site requests permission from the copyright holder before including the typefaces in commercial electronic products.

2000-01-01

3

Sp(2)-BRST  

SciTech Connect

A general method is given for the construction of gauge-fixed actions for theories with local gauge symmetries. The method is based on the single requirement that the space of fields carries an irreducible representation of the Sp(2)-BRST algebra, with respect to which the resultant actions are then automatically invariant.

Twisk, S.; Zhang, R.B.

1988-09-01

4

Dihydroramulosin from Botrytis sp.  

PubMed

Botrytis sp., isolated from the inner bark of the Pacific yew, Taxus brevifolia, was shown to produce ramulosin (1), 6-hydroxyramulosin (2), and the new compound 8-dihydroramulosin (3). The structure of dihydroramulosin was deduced from the NMR spectra and confirmed by chemical conversion from ramulosin. PMID:9784167

Stierle, D B; Stierle, A A; Kunz, A

1998-10-01

5

SP mountain data analysis  

NASA Technical Reports Server (NTRS)

An analysis of synthetic aperture radar data of SP Mountain was undertaken to demonstrate the use of digital image processing techniques to aid in geologic interpretation of SAR data. These data were collected with the ERIM X- and L-band airborne SAR using like- and cross-polarizations. The resulting signal films were used to produce computer compatible tapes, from which four-channel imagery was generated. Slant range-to-ground range and range-azimuth-scale corrections were made in order to facilitate image registration; intensity corrections were also made. Manual interpretation of the imagery showed that L-band represented the geology of the area better than X-band. Several differences between the various images were also noted. Further digital analysis of the corrected data was done for enhancement purposes. This analysis included application of an MSS differencing routine and development of a routine for removal of relief displacement. It was found that accurate registration of the SAR channels is critical to the effectiveness of the differencing routine. Use of the relief displacement algorithm on the SP Mountain data demonstrated the feasibility of the technique.

Rawson, R. F.; Hamilton, R. E.; Liskow, C. L.; Dias, A. R.; Jackson, P. L.

1981-01-01

6

NASA SP-4009 APOLLO SPACECRAFT  

E-print Network

NASA SP-4009 THE APOLLO SPACECRAFT VOLUME IV January 21, 1966-July 13, 1974 hy Ivan D. Ertel (revised) Main entry under title: The Apollo spacecraft. (The NASA historical series) (NASA SP-4009. 8, 1962-Sept. 30, 1964. [etc.] Includes bibliographical references. 1. Project Apollo. I. Ertel

Rathbun, Julie A.

7

Acetobacter intermedius, sp. nov.  

PubMed

Strains of a new species in the genus Acetobacter, for which we propose the name A. intermedius sp. nov., were isolated and characterized in pure culture from different sources (Kombucha beverage, cider vinegar, spirit vinegar) and different countries (Switzerland, Slovenia). The isolated strains grow in media with 3% acetic acid and 3% ethanol as does A. europaeus, do, however, not require acetic acid for growth. These characteristics phenotypically position A. intermedius between A. europaeus and A. xylinus, DNA-DNA hybridizations of A. intermedius-DNA with DNA of the type strains of Acetobacter europaeus, A. xylinus, A. aceti, A. hansenii, A. liquefaciens, A. methanolicus, A. pasteurianus, A. diazotrophicus, Gluconobacter oxydans and Escherichia coli HB 101 indicated less than 60% DNA similarity. The important features of the new species are described. Acetobacter intermedius strain TF2 (DSM11804) isolated from the liquid phase of a tea fungus beverage (Kombucha) is the type strain. PMID:13678040

Boesch, C; Trcek, J; Sievers, M; Teuber, M

1998-03-01

8

DADiSP processing guide  

NASA Technical Reports Server (NTRS)

A guide for DADiSP software, intended for use by the Lambda Point Experiment (LPE) Team during and after the United States Microgravity Payload (USMP)-1 mission, is presented. DADiSP is a Data Analysis and Display Software developed and marketed by DSP Development Corporation, Cambridge, Massachusetts. This guide is intended to be used in addition to the DADiSP Worksheet User Manual and Reference Manual which are supplied by the company with the software. Technical support for DADiSP is available from DSP at (617) 577-1133. Access to DADiSP on Acceleration Characterization and Analysis Project (ACAP) EGSE is being provided to the LPE team during USMP-1 for off-line processing of SAMS data.

Rogers, Melissa J. B.

1993-01-01

9

SP-100 space reactor safety  

SciTech Connect

The SP-100 space reactor power system is being developed to meet the large electrical power requirements of civilian and military missions planned for the 1990's and beyond. It will remove the restrictions on electrical power generation that have tended to limit missions and will enable the fuller exploration and utilization of space. This booklet describes the SP-100 space reactor power system and its development. Particular emphasis is given to safety. The design aand operational features as well as the design and safety review process that will assure that the SP-100 can be launched nd operated safely are described.

Not Available

1987-05-01

10

Actinoplanes couchii sp. nov.  

PubMed

A Gram-positive bacterium, strain GW8-1761(T), was isolated from soil close to the Marmore waterfalls, Terni, Italy. 16S rRNA gene sequence similarity studies showed that strain GW8-1761(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes italicus JCM 3165(T) (98.9 %), A. rectilineatus IFO 13941(T) (98.5 %), A. palleronii JCM 7626(T) (97.8 %), A. utahensis IFO 13244(T) (97.6 %) and A. cyaneus DSM 46137(T) (97.6 %). Strain GW8-1761(T) could be distinguished from any other Actinoplanes species with validly published names by 16S rRNA gene sequence similarity values of less than 97.5 %. Chemotaxonomic data [major menaquinone MK-9(H(4)); major polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol, with phosphatidylcholine and aminoglycolipids absent; major fatty acids C(15 : 0), C(16 : 0), C(16 : 0) iso, C(17 : 1)omega8c and summed feature 3 (C(16 : 1)omega7c and/or C(15 : 0) iso 2-OH)] supported the affiliation of strain GW8-1761(T) to the genus Actinoplanes. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW8-1761(T) from the most closely related species. Strain GW8-1761(T) therefore merits species status, and we propose the name Actinoplanes couchii sp. nov., with the type strain GW8-1761(T) (=DSM 45050(T)=CIP 109316(T)). PMID:17392194

Kämpfer, Peter; Huber, Birgit; Thummes, Kathrin; Grün-Wollny, Iris; Busse, Hans-Jürgen

2007-04-01

11

Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera  

PubMed Central

We previously discovered a symbiotic lactic acid bacterial (LAB) microbiota in the honey stomach of the honeybee Apis mellifera. The microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. 16S rRNA gene sequence analyses and phenotypic and genetic characteristics revealed that the phylotypes isolated represent seven novel species. One grouped with Lactobacillus kunkeei and the others belong to the Lactobacillus buchneri and Lactobacillus delbrueckiisubgroups of Lactobacillus. We propose the names Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov. for these novel species, with the respective type strains being Fhon13NT (?=?DSM 26257T?=?CCUG 63287T), Bin4NT (?=?DSM 26254T?=?CCUG 63291T), Hon2NT (?=?DSM 26255T?=?CCUG 63289T), Hma8NT (?=?DSM 26256T?=?CCUG 63629T), Hma2NT (?=?DSM 26263T?=?CCUG 63633T), Bma5NT (?=?DSM 26265T?=?CCUG 63301T) and Biut2NT (?=?DSM 26262T?=?CCUG 63631T). PMID:24944337

Alsterfjord, Magnus; Nilson, Bo; Butler, Eile; Vasquez, Alejandra

2014-01-01

12

Argonne's SpEC Module  

ScienceCinema

Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

Harper, Jason

2014-06-05

13

The Sp(1)-Kepler Problems  

E-print Network

Let $n\\ge 2$ be a positive integer. To each irreducible representation $\\sigma$ of $\\mathrm{Sp}(1)$, an $\\mathrm{Sp}(1)$-Kepler problem in dimension $(4n-3)$ is constructed and analyzed. This system is super integrable and when $n=2$ it is equivalent to a generalized MICZ-Kepler problem in dimension five. The dynamical symmetry group of this system is $\\widetilde {\\mathrm O}^*(4n)$ with the Hilbert space of bound states ${\\mathscr H}(\\sigma)$ being the unitary highest weight representation of $\\widetilde {\\mathrm {O}^*}(4n)$ with highest weight $$(\\underbrace{-1, ..., -1}_{2n-1}, -(1+\\bar\\sigma)),$$ which occurs at the right-most nontrivial reduction point in the Enright-Howe-Wallach classification diagram for the unitary highest weight modules. Here $\\bar\\sigma$ is the highest weight of $\\sigma$. Furthermore, it is shown that the correspondence $\\sigma\\leftrightarrow \\mathscr H(\\sigma)$ is the theta-correspondence for dual pair $(\\mathrm{Sp}(1), \\mathrm{O}^*(4n))\\subseteq\\mathrm{Sp}_{8n}(\\mathbb R)$.

Guowu Meng

2008-05-07

14

Leaving the fullerene road: presence and stability of sp chains in sp2  

E-print Network

Leaving the fullerene road: presence and stability of sp chains in sp2 carbon clusters and cluster carbon sp chains are essential ingredients for the formation of carbon fullerenes and nanotubes [6, 7 identified as a fundamental ingredient to transform sp structures into fullerenes and nanotubes

Powles, Rebecca

15

Pseudonocardia cypriaca sp. nov., Pseudonocardia salamisensis sp. nov., Pseudonocardia hierapolitana sp. nov. and Pseudonocardia kujensis sp. nov., isolated from soil.  

PubMed

The taxonomic positions of four novel actinomycetes isolated from soil samples, designated KT2142T, PM2084T, K236T and A4038T, were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus Pseudonocardia. Whole-cell hydrolysates of the four strains contained meso-diaminopimelic acid and arabinose and galactose as the diagnostic sugars (cell-wall type IV). Their predominant menaquinone was found to be MK-8(H4). The major fatty acid was iso-C16:0. 16S rRNA gene sequence data supported the classification of the isolates in the genus Pseudonocardia and showed that they formed four distinct branches within the genus. DNA-DNA relatedness studies between the isolates and their phylogenetic neighbours showed that they belonged to distinct genomic species. The four isolates were readily distinguished from one another and from the type strains of species classified in the genus Pseudonocardia based on a combination of phenotypic and genotypic properties. In conclusion, it is proposed that the four isolates be classified in four novel species of the genus Pseudonocardia, for which the names Pseudonocardia cypriaca sp. nov. (type strain KT2142T=KCTC 29067T=DSM 45511T=NRRL B-24882T), Pseudonocardia hierapolitana sp. nov. (type strain PM2084T=KCTC 29068T=DSM 45671T=NRRL B-24879T), Pseudonocardia salamisensis sp. nov. (type strain K236T=KCTC 29100T=DSM 45717T) and Pseudonocardia kujensis sp. nov. (type strain A4038T=KCTC 29062T=DSM 45670T=NRRL B-24890T) are proposed. PMID:24523445

Sahin, Nevzat; Veyisoglu, Aysel; Tatar, Demet; Spröer, Cathrin; Cetin, Demet; Guven, Kiymet; Klenk, Hans-Peter

2014-05-01

16

Antifungal macrodiolide from Streptomyces sp.  

PubMed Central

Aerobic fermentation cultures of Streptomyces sp. produced an antifungal macrodiolide. This new antibiotic consists of two units of homononactic acid linked to form a cyclic diester. An unknown polypeptide was also isolated in trace quantities. The antibiotic with polypeptide complex showed high levels of antifungal activity compared with that of the macrodiolide alone. The macrodiolide also showed a stimulatory effect on some species of fungi. The production, purification, and characterization of these compounds are reported. PMID:3777909

Jois, H R; Sarkar, A; Gurusiddaiah, S

1986-01-01

17

Actinoplanes liguriensis sp. nov. and Actinoplanes teichomyceticus sp. nov.  

PubMed

The taxonomic status of 'Actinoplanes liguriae' A/6353 and 'Actinoplanes teichomyceticus' AB8327 was established by using a polyphasic approach. Strains A/6353 and AB8327 form distinct phylogenetic lineages in the 16S rRNA gene tree of members of the genus Actinoplanes and are related moderately and closely to Actinoplanes rectilineatus and Actinoplanes cyaneus, respectively. Morphological, cultural and physiological properties indicated that strains A/6353 and AB8327 represent separate, novel species of the genus Actinoplanes, Actinoplanes liguriensis sp. nov. (type strain A/6353(T)=FH 2244(T)=DSM 43865(T)=ATCC 31048(T)=BCRC 12121(T)=CBS 355.75(T)=IMSNU 22127(T)=JCM 3250(T)=KCTC 9536(T)=KCC A-0250(T)=NBRC 13997(T)=NCIMB 12636(T)=NRRL B-16723(T)=SANK 62178(T)) and Actinoplanes teichomyceticus sp. nov. (type strain AB8327(T)=FH 2149(T)=DSM 43866(T)=ATCC 31121(T)=BCRC 12106(T)=FERM P-3462(T)=IMSNU 20043(T)=IMET 9254(T)=JCM 3252(T)=KCC A-0252(T)=KCTC 9543(T)=NBRC 13999(T)=NCIMB 12640(T)=NRRL B-16726(T)=SANK 60479(T)). PMID:16957109

Wink, Joachim M; Kroppenstedt, Reiner M; Schumann, Peter; Seibert, Gerhard; Stackebrandt, Erko

2006-09-01

18

Lactobacillus pasteurii sp. nov. and Lactobacillus hominis sp. nov.  

PubMed

Strains 1517(T) and 61D(T) were characterized by phenotypic and molecular taxonomic methods. These Gram-positive lactic acid bacteria were homo-fermentative, facultatively anaerobic short rods. They were phylogenetically related to the genus Lactobacillus according to 16S rRNA gene sequence analysis, with 99 % similarity between strain 1517(T) and the type strain of Lactobacillus gigeriorum, and 98.6, 98.5 and 98.4 % between strain 61D(T) and Lactobacillus gasseri, Lactobacillus taiwanensis and Lactobacillus johnsonii, respectively. Multilocus sequence analysis and metabolic analysis of both strains showed variation between the two strains and their close relatives, with variation in the position of the pheS and rpoA genes. The DNA-DNA relatedness of 43.5 % between strain 1517(T) and L. gigeriorum, and 38.6, 29.9 and 39.7 % between strain 61D(T) and L. johnsonii, L. taiwanensis and L. gasseri, respectively, confirmed their status as novel species. Based on phenotypic and genotypic characteristics, two novel species of Lactobacillus are proposed: Lactobacillus pasteurii sp. nov., with 1517(T) ( = CRBIP 24.76(T) = DSM 23907(T)) as the type strain, and Lactobacillus hominis sp. nov., with 61D(T) (=CRBIP 24.179(T) = DSM 23910(T)) as the type strain. PMID:22328611

Cousin, Sylvie; Motreff, Laurence; Gulat-Okalla, Marie-Laure; Gouyette, Catherine; Spröer, Cathrin; Schumann, Peter; Begaud, Evelyne; Bouchier, Christiane; Clermont, Dominique; Bizet, Chantal

2013-01-01

19

Methylobacterium persicinum sp. nov., Methylobacterium komagatae sp. nov., Methylobacterium brachiatum sp. nov., Methylobacterium tardum sp. nov. and Methylobacterium gregans sp. nov., isolated from freshwater.  

PubMed

Eight strains, 002-165T, 002-079T, B0021T, Hojyo2, RB603B, RB677T, 002-074T and RB678, isolated from the environment of food-processing factories in Japan, were characterized using a polyphasic approach. The isolates were Gram-negative, strictly aerobic, pink-pigmented, facultatively methylotrophic, non-spore-forming rods. The chemotaxonomic characteristics of these isolates included the presence of C18 : 1omega7c as the major cellular fatty acid and ubiquinone Q-10 as the predominant ubiquinone. The DNA G+C content was 67.1-71.1 mol%. Phylogenetic analyses of 16S rRNA and DNA gyrase B subunit (gyrB) nucleotide sequence confirmed that the eight strains belonged to the Methylobacterium clade. Moreover, a DNA-DNA hybridization analysis showed that the eight isolates represented five novel species. On the basis of their phenotypic and phylogenetic distinctiveness, the isolates represent five novel species within the genus Methylobacterium, for which the names Methylobacterium persicinum sp. nov. (type strain 002-165T =DSM 19562T =NBRC 103628T =NCIMB 14378T), Methylobacterium komagatae sp. nov. (type strain 002-079T =DSM 19563T =NBRC 103627T =NCIMB 14377T), Methylobacterium brachiatum sp. nov. (type strain B0021T =DSM 19569T =NBRC 103629T =NCIMB 14379T), Methylobacterium tardum sp. nov. (type strain RB677T =DSM 19566T =NBRC 103632T =NCIMB 14380T) and Methylobacterium gregans sp. nov. (type strain 002-074T =DSM 19564T =NBRC 103626T =NCIMB 14376T) are proposed. PMID:18450702

Kato, Yuko; Asahara, Mika; Goto, Keiichi; Kasai, Hiroaki; Yokota, Akira

2008-05-01

20

Modulation of biocidal activity of Calothrix sp. and Anabaena sp. by environmental factors  

Microsoft Academic Search

An investigation was undertaken to evaluate a set of cyanobacterial strains in terms of production of biocidal compounds exhibiting\\u000a allelochemical and fungicidal properties. Two cyanobacterial strains — Anabaena sp. and Calothrix sp. were selected for further investigation, on the basis of their larger inhibition zones on the lawn of Synechocystis and Synechococcus sp. and two phytopathogenic fungi — Rhizoctonia bataticola

Balasubramanian Radhakrishnan; Radha Prasanna; Pranita Jaiswal; Saswati Nayak; Prem Dureja

2009-01-01

21

Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov.  

PubMed

Two thermophilic bacilli were isolated from mud and water samples of the Ayder and Kestanbol hot springs in the provinces of Rize and Canakkale, respectively, in Turkey. Strains AB04T and K4T were sporulating, Gram-positive, rod-shaped bacteria. These isolates were moderately thermophilic (with an optimum temperature for growth of 50-55 degrees C), facultative anaerobes able to grow on a wide range of carbon sources including d-glucose, d-raffinose, d-sucrose, D-xylose, D-fructose, L-arabinose, maltose, D-mannose and D-mannitol. Analysis of the 16S rRNA gene sequences showed that these isolates resembled Anoxybacillus flavithermus DSM 2641T and Anoxybacillus gonensis NCIMB 13933T. DNA-DNA hybridization data revealed that thermophilic isolate AB04T has only 51.2 % relatedness to A. flavithermus, 45.1 % relatedness to Anoxybacillus pushchinoensis and 68.6 % relatedness to A. gonensis. Thermophilic isolate K4T showed only 60.4 % relatedness to A. flavithermus, 42.9 % relatedness to A. pushchinoensis and 38.5 % relatedness to A. gonensis. On the basis of the DNA-DNA hybridization data, isolates AB04T and K4T are not related to A. flavithermus DSM 2641T, A. pushchinoensis DSM 12423T or A. gonensis NCIMB 13933T at the species level, but show relatedness to one another of 40.5 %. On the basis of the data presented, it is proposed that strains AB04T (= NCIMB 13972T = NCCB 100050T) and K4T (= NCIMB 13971T = NCCB 100051T) be designated as the type strains of Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov., respectively. PMID:15388701

Dulger, Sabriye; Demirbag, Zihni; Belduz, Ali Osman

2004-09-01

22

Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov.  

PubMed

Gram-negative, non-spore-forming, rod-shaped bacteria, T7-09(T) and TR6-08(T), were isolated from soil from a ginseng field in South Korea and characterized to determine their taxonomic position. 16S rRNA gene sequence analysis showed that the two isolates shared 99.5 % sequence similarity. Strains T7-09(T) and TR6-08(T) were shown to belong to the Proteobacteria and showed the highest levels of sequence similarity to Pseudoxanthomonas broegbernensis DSM 12573(T) (98.1 %), Pseudoxanthomonas mexicana AMX 26B(T) (97.4-97.5 %), Pseudoxanthomonas japonensis 12-3(T) (96.5-96.6 %), Pseudoxanthomonas taiwanensis ATCC BAA-404(T) (95.7 %) and Xanthomonas campestris ATCC 33913(T) (96.3-96.5 %). The sequence similarity values with respect to any species with validly published names in related genera were less than 96.5 %. The detection of a quinone system with Q-8 as the predominant compound and a fatty acid profile with C(15 : 0) iso as the predominant acid supported the assignment of the novel isolates to the order 'Xanthomonadales'. The two isolates could be distinguished from the established species of the genus Pseudoxanthomonas by the presence of quantitative unsaturated fatty acid C(17 : 1) iso omega9c and by their unique biochemical profiles. The results of DNA-DNA hybridization clearly demonstrated that T7-09(T) and TR6-08(T) represent separate species. On the basis of these data, it is proposed that T7-09(T) (=KCTC 12208(T)=IAM 15116(T)) and TR6-08(T) (=KCTC 12207(T)=IAM 15115(T)) be classified as the type strains of two novel Pseudoxanthomonas species, for which the names Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov., respectively, are proposed. PMID:15774663

Yang, Deok-Chun; Im, Wan-Taek; Kim, Myung Kyum; Lee, Sung-Taik

2005-03-01

23

_q .. SP-6102 -" IN SYSTEMS ENGINEERING  

E-print Network

_�q .. SP-6102 -" READINGS IN SYSTEMS ENGINEERING Edited by Francis T. Hoban and William M. Lawbaugh co ! (NASA-SP-6102) REAOINGS IN SYSTEMS ENGINEERING (NASa) 215 p N93-24678 --THRU-- N93-24693 Unclas H1/31 0158570 #12;.J T ,j J #12;READINGS IN SYSTEMS ENGINEERING Edited by Francis T. Hoban

Rhoads, James

24

Launch vehicle integration requirements for SP-100  

NASA Technical Reports Server (NTRS)

SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS) Space Shuttle System into a near-earth orbit, deployed, and through upper stage propulsion burn(s) be inserted/transferred to its mission orbit. The nature of the advanced design SP-100 gives rise to a set of issues that require special attention to assure that payloads using this power plant are physically and functionally compatible with the NSTS and meet the safety requirements thereof. The purpose of this document is to define and present the requirements and interface provisions that, when satisfied, will ensure technical compatibility between SP-100 systems and the NSTS.

Shaw, L. T., Jr.; Womack, J. R.

1984-01-01

25

S&P 100 Index Option Volatility  

Microsoft Academic Search

Using transaction data on the S&P 100 index options, the authors study the effect of valuation simplifications that are commonplace in previous research on the time-series properties of implied market volatility. Using an American-style algorithm that accounts for the discrete nature of the dividends on the S&P 100 index, they find that spurious negative serial correlation in implied volatility changes

Campbell R Harvey; Robert E Whaley

1991-01-01

26

Expression Patterns of SP1 and SP3 During Mouse Spermatogenesis  

PubMed Central

Because of their prominent roles in regulation of gene expression, it is important to understand how levels of Krüpple-like transcription factors SP1 and SP3 change in germ cells during spermatogenesis. Using immunological techniques we found that both factors decreased sharply during meiosis. SP3 declined during the leptotene to pachytene transition while SP1 fell somewhat later, as spermatocytes progressed beyond early pachytene. SP3 reappeared for a period in round spermatids. For Sp1 it is known that the transition to pachytene is accompanied by loss of the normal 8.2 kb mRNA and appearance of a prevalent 8.8 kb variant, which has not been well characterized. We have now shown that this pachytene-specific transcript contains a long, unspliced sequence from the first intron and that this sequence inhibits expression of a reporter, probably due to its many short open reading frames. A second testis-specific Sp1 transcript in spermatids of 2.4 kb has also been reported previously. Like the 8.8 kb variant, it is also translationally compromised. We have confirmed by Northern blotting that the 8.8, 8.2 and 2.4 kb variants account for the major testis Sp1 transcripts. Thus the unexpected decline of SP1 protein in the face of continuing Sp1 transcription is explained in large part by poor translation of both novel testis transcripts. As part of this work we also identified five additional minor Sp1 cap sites by 5? RACE, including a trans-spliced RNA originating from the Glcci1 gene. PMID:18417714

Ma, Wenli; Horvath, Gary C.; Kistler, Malathi K.; Kistler, W. Stephen

2008-01-01

27

Neophyllobius lorestanicus sp. nov. and N. ostovani sp.nov. (Acari: Camerobiidae) from Iran.  

PubMed

Two new species of the genus Neophyllobius Berlese, 1886 are described: Neophyllobius lorestanicus sp. nov. collected from soil under Prunus domestica L. (Rosaceae) in Markazi province and Neophyllobius ostovani sp. nov. from soil and rotten leaves of oak trees in Fars province, Iran. A key to all known Iranian and Turkish species of Neophyllobius is provided. PMID:24870646

Khanjani, Mohammad; Hoseini, Mohammad Ahmad; Yazdanpanah, Shima; Masoudian, Farshad

2014-01-01

28

Cheylostigmaeus tarae sp. nov. and Stigmaeus delaramae sp. nov. (Acari: Stigmaeidae) from Kurdistan, Iran.  

PubMed

Two new species belonging to the family Stigmaeidae, Cheylostigmaeus tarae sp. nov. and Stigmaeus delaramae sp. nov., are described from specimens collected from soil and litter under pear trees, Pyrus communis L. (Rosaceae) in Iran. A key to all Iranian species of the genera Cheylostigmaeus (male) and Stigmaeus (female) are provided.  PMID:25082045

Khanjani, Mohammad; Nasrollahi, Siamak; Zamani, Ali Sina; Fayaz, Bahman Asali

2014-01-01

29

Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel  

E-print Network

Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel Chlorella algal genus, Chlorella, which accommodated coccoid unicellular green algal species with globular to oval. These algae share the general Chlorella-like morphology and their 18S rRNA and rbcL gene sequences place them

30

Achromobacter animicus sp. nov., Achromobacter mucicolens sp. nov., Achromobacter pulmonis sp. nov. and Achromobacter spiritinus sp. nov., from human clinical samples.  

PubMed

The phenotypic and genotypic characteristics of fourteen human clinical Achromobacter strains representing four genogroups which were delineated by sequence analysis of nusA, eno, rpoB, gltB, lepA, nuoL and nrdA loci, demonstrated that they represent four novel Achromobacter species. The present study also characterized and provided two additional reference strains for Achromobacter ruhlandii and Achromobacter marplatensis, species for which, thus far, only single strains are publicly available, and further validated the use of 2.1% concatenated nusA, eno, rpoB, gltB, lepA, nuoL and nrdA sequence divergence as a threshold value for species delineation in this genus. Finally, although most Achromobacter species can be distinguished by biochemical characteristics, the present study also highlighted considerable phenotypic intraspecies variability and demonstrated that the type strains may be phenotypically poor representatives of the species. We propose to classify the fourteen human clinical strains as Achromobacter mucicolens sp. nov. (with strain LMG 26685(T) [=CCUG 61961(T)] as the type strain), Achromobacter animicus sp. nov. (with strain LMG 26690(T) [=CCUG 61966(T)] as the type strain), Achromobacter spiritinus sp. nov. (with strain LMG 26692(T) [=CCUG 61968(T)] as the type strain), and Achromobacter pulmonis sp. nov. (with strain LMG 26696(T) [=CCUG 61972(T)] as the type strain). PMID:23219252

Vandamme, Peter; Moore, Edward R B; Cnockaert, Margo; De Brandt, Evie; Svensson-Stadler, Liselott; Houf, Kurt; Spilker, Theodore; Lipuma, John J

2013-02-01

31

[Sulfation of naringenin by Mucor sp].  

PubMed

Naringenin (1) was transformed to three metabolites (2-4) by Mucor sp. Based on LCMS(n)-IT-TOF and NMR spectroscopic data, 2-4 were identified as naringenin-7-O-sulphate, naringenin-4'-O-sulphate, and naringenin-5-O-sulphate, respectively. These results might provide hints to the mammalian/human metabolism of naringenin. PMID:25272838

Ruan, Fei-Ying; Chen, Ri-Dao; Li, Jian-Hua; Zhang, Min; Xie, Ke-Bo; Wang, Yan; Feng, Ru; Dai, Jun-Gui

2014-06-01

32

Process Engineering Thermodynamics 424304 E (4 sp)  

E-print Network

F (mol/mol), 3) the fraction of the feed that is liquid (i.e. q), and 4) the temperature of the feed. cProcess Engineering Thermodynamics 424304 E (4 sp) Exam 9-3-2009 All support material is allowed questions are given, hand in answers for only (i.e. more than) 4 questions. Choose as you wish. In all

Zevenhoven, Ron

33

Biopolymer flocculant produced by an Enterobacter sp  

Microsoft Academic Search

A new biopolymer flocculant was produced by Enterobacter sp. BY-29. Flocculating activity increased in the presence of Al , Fe or Fe . The flocculant had flocculating activity not only in inorganic suspensions of kaolin and active carbon but also in organic suspensions of cellulose and yeast. The flocculant was an acidic polysaccharide consisting of glucose, galactose, xylose and galacturonic

Haruhiko Yokoi; Takashi Yoshida; Shingo Mori; Jun Hirose; Sachio Hayashi; Yoshiyuki Takasaki

1997-01-01

34

Power transmission studies for tethered SP-100  

NASA Technical Reports Server (NTRS)

The tether and/or transmission line connecting the SP-100 to Space Station presents some unorthodox challenges in high voltage engineering, power transmission, and distribution. The line, which doubles as a structural element of this unusual spacecraft, will convey HVDC from SP-100 to the platform in low Earth orbit, and environment where the local plasma is sufficient to cause breakdown of exposed conductors at potentials of only a few hundred volts. Its anticipated several years operation, and continuously accumulating exposure to meteoroids and debris, raises an increasing likelihood that mechanical damage, including perforation, will be sustained in service. The present concept employs an array of gas insulated solid wall aluminum coaxial tubes; a conceptual design which showed basic feasibility of the SP-100 powered Space Station. Practical considerations of launch, deployment and assembly have led to investigation of reel deployable, dielectric insulated coaxial cables. To be competitive, the dielectric would have to operate reliably in a radiation environment under electrical stresses exceeding 50 kV/cm. The SP-100 transmission line high voltage interfaces are also considered.

Bents, David J.

1988-01-01

35

Power transmission studies for tethered SP-100  

NASA Technical Reports Server (NTRS)

The tether and/or transmission line connecting the SP-100 to space station presents some unorthodox challenges in high voltage engineering, power transmission, and distribution. The line, which doubles as a structural element of this unusual spacecraft, will convey HVDC from SP-100 to the platform in low Earth orbit, and environment where the local plasma is sufficient to cause breakdown of exposed conductors at potentials of only a few hundred volts. Its anticipated several years operation, and continuously accumulating exposure to meteoroids and debris, raises an increasing likelihood that mechanical damage, including perforation, will be sustained in service. The present concept employs an array of gas insulated solid wall aluminum coaxial tubes; a conceptual design which showed basic feasibility of the SP-100 powered space station. Practical considerations of launch, deployment and assembly have lead to investigation of reel deployable, dielectric insulated coaxial cables. To be competitive, the dielectric would have to operate reliably in a radiation environment under electrical stresses exceeding 50 kV/cm. The SP-100 transmission line high voltage interfaces are also considered.

Bents, David J.

1988-01-01

36

Lagenidium sp. Ocular Infection Mimicking Ocular Pythiosis  

PubMed Central

This is a report of a Lagenidium sp. in a Thai patient who was diagnosed with severe keratitis that was unresponsive to antibacterial and antifungal drugs. Examination of a corneal biopsy specimen confirmed the presence of aseptate hyphae. The internal transcribed spacer DNA sequence of the strain isolated showed 97% identity with Lagenidium giganteum and other Lagenidium species. PMID:23740721

Reinprayoon, Usanee; Permpalung, Nitipong; Plongla, Rongpong; Mendoza, Leonel; Chindamporn, Ariya

2013-01-01

37

Uranium accumulation by Pseudomonas sp. EPS5028  

Microsoft Academic Search

Pseudomonas sp. EPS-5028 was examined for the ability to accumulate uranium from solutions. The uptake of uranium by this microorganism is very rapid and is affected by pH but not by temperature, metabolic inhibitors, culture time and the presence of various cations and anions. The amount of uranium absorbed by the cells increased as the uranium concentration of the solution

Ana M. Marqués; Xavier Roca; M. Dolores Simon-Pujol; M. Carmen Fuste; Francisco Congregado

1991-01-01

38

MAGNET CELL SAFETY PROCEDURE SP-16 Revision 02  

E-print Network

MAGNET CELL SAFETY PROCEDURE SP-16 Page i Revision 02 July 19, 2006 NATIONAL HIGH MAGNETIC: MAGNET CELL SAFETY PROCEDURE ______________________________________________________ HEAD of MAGNET ______________________________________________________ HEAD of FACILITIES John Kynoch #12;MAGNET CELL SAFETY PROCEDURE SP-16 Pageii Revision 02 July 19

Weston, Ken

39

Genes encoding ferredoxins from Anabaena sp. PCC 7937 and Synechococcus sp. PCC 7942: structure and regulation  

Microsoft Academic Search

The gene encoding ferredoxin I (petF1) from the filamentous cyanobacterium Anabaena sp. PCC 7937 (Anabaena variabilis ATCC 29413) was cloned by low stringency hybridization with the ferredoxin cDNA from the higher plant Silene pratensis. The petF1 gene from the unicellular cyanobacterium Synechococcus sp. PCC 7942 (Anacystis nidulans R2) was cloned by low stringency hybridization with the petF1 gene from Anabaena

JAN VAN DER PLAS; ROLF DE GROOT; Martin Woortman; Fons Cremers; Mies Borrias; GERARD VAN ARKEL; Peter Weisbeek

1988-01-01

40

Factors Affecting Predation by Cyclidium sp. and Euplotes sp. on PAH-Degrading and Nondegrading Bacteria  

Microsoft Academic Search

If predators select for or against contaminant-degrading bacteria, it will affect bacterial survival and has important implications\\u000a for bioremediation. Protozoa are important predators of bacteria. In order to determine whether protozoa preyed differentially\\u000a on bacteria with different degradation abilities, two ciliates (Euplotes sp. and Cyclidium sp.) and three strains of PAH-degrading bacteria (Vibrio spp., degrading naphthalene, anthracene, or phenanthrene) were

S. F. Tso; G. L. Taghon

1999-01-01

41

Major Surface Glycoprotein Genes from Pneumocystis carinii f. sp. ratti  

Microsoft Academic Search

Pneumocystis carinii occurs in a variety of mammals, each of which harbors one or more genetically distinct “special forms” of the microbe. Laboratory rats can be infected by two special forms, P. carinii f. sp. ratti and P. carinii f. sp. carinii. P. carinii f. sp. carinii has a variable antigen, the major surface glycoprotein (MSG), the expression of which

Joshua K Schaffzin; Thomas R Garbe; James R Stringer

1999-01-01

42

Vibrio kanaloae sp. nov., Vibrio pomeroyi sp. nov. and Vibrio chagasii sp. nov., from sea water and marine animals.  

PubMed

The taxonomic position of the fluorescent amplified fragment length polymorphism fingerprinting groups A46 (five isolates), A51 (six isolates), A52 (five isolates) and A53 (seven isolates) obtained in a previous study were further analysed through a polyphasic approach. The 23 isolates were phylogenetically related to Vibrio splendidus, but DNA-DNA hybridization experiments proved that they belong to three novel species. Chemotaxonomic and phenotypic analyses further disclosed several features that differentiate between the 23 isolates and known Vibrio species. The names Vibrio kanaloae sp. nov. (type strain LMG 20539(T) = CAIM 485(T); EMBL accession no. AJ316193; G + C content 44.7 mol%), Vibrio pomeroyi sp. nov. (type strain LMG 20537(T) = CAIM 578(T); EMBL accession no. AJ491290; G +C content 44.1 mol%) and Vibrio chagasii sp. nov. (type strain LMG 21353(T) = CAIM 431(T); EMBL accession no. AJ316199; G + C content 44.6 mol%) are respectively proposed to encompass the five isolates of A46, the six isolates of A51 and the 12 isolates of A52/A53. The three novel species can be distinguished from known Vibrio species by several phenotypic features, including utilization and fermentation of various carbon sources, beta-galactosidase activity and fatty acid content (particularly of 12 : 0, 14: 0, 14 : 0 iso and 16 : 0 iso). PMID:12807197

Thompson, F L; Thompson, C C; Li, Y; Gomez-Gil, B; Vandenberghe, J; Hoste, B; Swings, J

2003-05-01

43

Experiments are described in which traditional computer-administered stated-preference (SP) data are compared with virtual experience SP  

E-print Network

the virtual experience SP data based on the driving simulator suggested that freeway delay is more onerous are compared with virtual experience SP data to ascertain how people value stopped delay compared with stop- and-go or free-flow traffic. The virtual experience SP experiments were conducted by using

Levinson, David M.

44

Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1  

SciTech Connect

Research highlights: {yields} Sp1 zinc fingers themselves interact with importin {alpha}. {yields} Sp1 zinc finger domains play an essential role as a nuclear localization signal. {yields} Sp1 can be transported into the nucleus in an importin-dependent manner. -- Abstract: Transcription factor Sp1 is localized in the nucleus and regulates the expression of many cellular genes, but the nuclear transport mechanism of Sp1 is not well understood. In this study, we revealed that GST-fused Sp1 protein bound to endogenous importin {alpha} in HeLa cells via the Sp1 zinc finger domains, which comprise the DNA binding domain of Sp1. It was found that the Sp1 zinc finger domains directly interacted with a wide range of importin {alpha} including the armadillo (arm) repeat domain and the C-terminal acidic domain. Furthermore, it turned out that all three zinc fingers of Sp1 are essential for binding to importin {alpha}. Taken together, these results suggest that the Sp1 zinc finger domains play an essential role as a NLS and Sp1 can be transported into the nucleus in an importin-dependent manner even though it possesses no classical NLSs.

Ito, Tatsuo [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan)] [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Kitamura, Haruka; Uwatoko, Chisana; Azumano, Makiko [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan)] [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan); Itoh, Kohji, E-mail: kitoh@ph.tokushima-u.ac.jp [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan)] [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Kuwahara, Jun, E-mail: jkuwahar@dwc.doshisha.ac.jp [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan)] [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan)

2010-12-10

45

A pumilio homolog in Polycelis sp.  

PubMed

Pumilio proteins (PUMs), members of the pumilio/fem-3 mRNA-binding factor (PUF) family, are eukaryote-specific RNA-binding proteins. We isolated a 2,048-basepair cDNA fragment of a pumilio homolog from the planarian flatworm Polycelis sp. This pumilio protein (PyPUM) contains a conserved pumilio homology domain (PUM-HD) consisting of eight repeats and two flanking half repeats. PyPUM shows high similarity to Dugesia japonica pumilio (DjPUM) from another planarian D. japonica, and their PUM-HD also shows high similarity to each other. Furthermore, our data showed that there is a flatworm-specific spacer between repeats 7 and 8. Phylogenetic analysis showed that PyPUM has a closer relationship to other PUM homologs from flatworms. These results provide a foundation for future functional studies of pumilio gene in Polycelis sp. PMID:24292205

Yuwen, Yanqing; Dong, Zimei; Si, Xiaohui; Chen, Guangwen

2014-02-01

46

Carbon sp chains in graphene nanoholes  

NASA Astrophysics Data System (ADS)

Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment.

Castelli, Ivano E.; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

2012-03-01

47

Commercial technologies from the SP-100 program  

NASA Astrophysics Data System (ADS)

For more than a decade, the Jet Propulsion Labortory (JPL) and Los Alamos National Laboratory (LANL) have managed a multi-agency funded effort to develop a space reactor power system. This SP-100 Program has developed technologies required for space power systems that can be implemented in the industrial and commercial sectors to improve our competitiveness in the global economy. Initial steps taken to transfer this technology from the laboratories to industrial and commercial entities within the United States include: (1) identifying specific technologies having commercial potential; (2) distributing information describing the identified technologies and interacting with interested commercial and industrial entities to develop application-specific details and requirements; and (3) providing a technological data base that leads to transfer of technology or the forming of teaming arrangements to accomplish the transfer by tailoring the technology to meet application-specific requirements. SP-100 technologies having commercial potential encompass fabrication processes, devices, and components. Examples are a process for bonding refractory metals to graphite, a device to sense the position of an actuator and a component to enable rotating machines to operate without supplying lubrication ( a self-lubricating ball bearing). Shortly after the National Aeronautics and Space Administration (NASA) Regional Technology Transfer Centers widely disseminated information covering SP-100 technologies, over one hundred expressions of interest were received. These early responses indicate that there is a large potential benefit in transferring SP-100 technology. Interactions with industrial and commercial entities have identified a substantial need for creating teaming arrangements involving the interested entity and personnel from laboratories and their contractors, who have the knowledge and ability to tailor the technology to meet application-specific requirements.

Truscello, Vincent C.; Fujita, Toshio; Mondt, Jack F.

1995-01-01

48

Process Engineering Thermodynamics 424304 E (4 sp)  

E-print Network

Process Engineering Thermodynamics 424304 E (4 sp) Exam 20-3-2013 All support material is allowed except for telecommunication devices. Below 6 questions are given, hand in answers for only (i.e. more is obtained at 27 ºC, 5 bar; the nitrogen is obtained at 30ºC, 2 bar. Data: for O2: cp = 29.4 kJ/kmol·K, for N

Zevenhoven, Ron

49

Chlamydomonas sajao nov. sp. (Chlorophyta, Volvocales)  

NASA Astrophysics Data System (ADS)

A new species of Chlamydomonas, namely, C. sajao nov. sp. of the Volvocales, Chlorophyta was isolated from a duckweed growing near a ricefield in the vicinity of Guangzhou, China. This interesting unicellular green alga, similar to C. mexicana from Mexico, secretes quantities of extracellular mucilaginous polysaccharides, and may be employed in improving soil quality. The new species resembles C. waldenburgensis Moewus in most characteristics but differs in three important features.

Lewin, Ralph A.

1984-06-01

50

Microbial flocculant from Arcuadendron sp. TS49  

Microsoft Academic Search

A flocculant was purified from the culture broth of Archuadendron sp. TS-49 by a series of precipitations with acetone, 60% ammonium sulfate-butanol, salting-out by dialysis, and cetylpyridinium chloride. The flocculating activity was observed most highly at pH 3.0 and markedly enhanced by the addition of salts, especially in the case of FeCl3 or FeSO4. This bioflocculant efficiently flocculated all tested

Soon Ho Lee; Sang Ok Lee; Kyung Lib Jang; Tae Ho Lee

1995-01-01

51

New phomopsolides from a Penicillium sp.  

PubMed

Investigation of the bioactive compounds from a Penicillium sp. isolated from the inner bark of the Pacific yew, Taxus brevifolia, led to the isolation of the known furanone 1, and a series of phomopsolides. The phomopsolide fractions contained phomopsolides A and B, which have previously been described, and three new phomopsolides. The structures of the new phomopsolides were deduced by comparison of their NMR spectra to those of the known compounds. PMID:9392888

Stierle, D B; Stierle, A A; Ganser, B

1997-11-01

52

SP-100 design, safety, and testing  

NASA Technical Reports Server (NTRS)

The SP-100 Program is developing a nuclear reactor power system that can enhance and/or enable future civilian and military space missions. The program is directed to develop space reactor technology to provide electrical power in the range of tens to hundreds of kilowatts. The major nuclear assembly test is to be conducted at the Hanford Site near Richland, Washington, and is designed to validate the performance of the 2.4-MWt nuclear and heat transport assembly.

Cox, Carl. M.; Mahaffey, Michael M.; Smith, Gary L.

1991-01-01

53

Taxonomic Study of Bacteria Isolated from Plants: Proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp. nov., and Sphingomonas mali sp. nov  

Microsoft Academic Search

The taxonomic positions of 10 strains of 3-ketolactose-forming bacteria which were isolated from the roots of plants (Rosa sp., Psychotria nairobiensis, Ardisiu crispa, Prunus persica, and apple trees) were investigated. The DNA base compositions of these strains ranged from 64.0 to 65.7 mol%, the isoprenoid quinone of each strain was ubiquinone 10,3-hydroxy fatty acids were lacking in the cellular fatty

MARIKO TAKEUCHI; TAKESHI SAKANE; MIYOKO YANAGI; KOEI HAMANA; AKIRA YOKOTA

54

SP 501 Communication as Christian Rhetoric  

Microsoft Academic Search

Holy Bible. (Please choose a translation to work from rather than a paraphrase). Schmit, Clayton. The Public Reading of Scripture. Nashville: Abingdon, 2002. ISBN:06-870-45-371 SP 501 Communication as Christian Rhetoric Syllabus Page 2 Schultze, Q. J. An Essential Guide to Public Speaking: Serving Your Audience with Faith, Skill, and Virtue. Grand Rapids: Baker, 2006. ISBN 08-010-31-516 Volf, Miroslav. Free of

Stacy R. Minger

2008-01-01

55

Lasionectrin, a naphthopyrone from a Lasionectria sp.  

PubMed

A new naphthopyrone derivative, lasionectrin (1), was isolated from fermentations of an Acremonium-like fungus provisionally identified as a Lasionectria sp. (Ascomycota, Hypocreales) and isolated from forest leaf litter from Equatorial Guinea. Its structure was determined by a combination of spectroscopic techniques, including UV, (+)-HRESIMS, and 1D and 2D NMR spectroscopy, and comparison with published data for related fungal metabolites. Compound 1 inhibited the growth of Plasmodium falciparum with an IC(50) value of 11 ?M. PMID:22694295

El Aouad, Noureddine; Pérez-Moreno, Guiomar; Sánchez, Paula; Cantizani, Juan; Ortiz-López, Francisco Javier; Martín, Jesús; González-Menéndez, Víctor; Ruiz-Pérez, Luis M; González-Pacanowska, Dolores; Vicente, Francisca; Bills, Gerald; Reyes, Fernando

2012-06-22

56

Sp6 and Sp8 transcription factors control AER formation and dorsal-ventral patterning in limb development.  

PubMed

The formation and maintenance of the apical ectodermal ridge (AER) is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6-/-;Sp8+/-) exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/?catenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning. PMID:25166858

Haro, Endika; Delgado, Irene; Junco, Marisa; Yamada, Yoshihiko; Mansouri, Ahmed; Oberg, Kerby C; Ros, Marian A

2014-08-01

57

SP10 Infectivity Is Aborted after Bacteriophage SP10 Infection Induces nonA Transcription on the Prophage SP? Region of the Bacillus subtilis Genome  

PubMed Central

Bacteria have developed various strategies for phage resistance. Infection with phage induces the transcription of part of the phage resistance gene, but the regulatory mechanisms of such transcription remain largely unknown. The phage resistance gene nonA is located on the SP? prophage region of the Bacillus subtilis Marburg strain genome. The nonA transcript was detected at the late stage of SP10 infection but is undetectable in noninfected cells. The nonA transcript was detected after the induction of the sigma factor Orf199-Orf200 (?Orf199-200), when sigma factors encoded in the SP10 genome were expressed from a xylose-inducible plasmid. Thus, the SP10 sigma factor is an activator of a set of SP10 genes and nonA. The nonA gene encodes a 72-amino-acid protein with a transmembrane motif and has no significant homology with any protein in any database. NonA overexpression halted cell growth and reduced the efficiency of B. subtilis colony formation and respiration activity. In addition, SP10 virion protein synthesis was inhibited in the nonA+ strain, and SP10 virion particles were scarce in it. These results indicate that NonA is a novel protein that can abort SP10 infection, and its transcription was regulated by SP10 sigma factor. PMID:24272782

Yamamoto, Tatsuya; Obana, Nozomu; Yee, Lii Mien; Asai, Kei; Nomura, Nobuhiko

2014-01-01

58

Pasteuria sp. Parasitizing Trophonema okamotoi in Florida.  

PubMed

Two populations of Trophonema okamotoi parasitized by Pasteuria sp. were found on Liquidambar styraciflua (sweetgum) and on an unidentified tropical grass in north-central Florida. Endospores of this Pasteuria sp. attached to motile vermiform second-stage juveniles (J2) and males of T. okamotoi, but not to other developmental stages. Sporangia and new endospores were produced only inside the bodies of swollen and sedentary third- and fourth-stage juveniles and females that developed in the host roots. No egg masses were produced by infected T. okamotoi females. The endospore diameter from the tropical grass population was 4.93 mum and the central core diameter was 1.97 mum; measurements of endospores from the sweetgum populations were similar. Endospores that were collected from T. okamotoi and added to uninfected T. okamotoi and other plant-parasitic nematodes attached/to J2 of T. okamotoi but did not attach to juveniles and adults of Helicotylenchus pseudorotrustus, Pratylenchus brachyurus, or to J2 of either Meloidogyne arenaria race 1, M. incognita race 1, M. javanica, or Tylenchulus semipenetrans. Pasteuria sp. from T. okamotoi differed from the described Pasteuria species in endospore size, host preference, and rate of attachment. PMID:19283199

Inserra, R N; Oostendorp, M; Dickson, D W

1992-03-01

59

Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp.  

PubMed

Environments contaminated with heavy metals negatively impact the living organisms. Ectomycorrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L(-1) in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L(-1) in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated for 30 days at 28 °C. UFSC-Pt116 showed high copper-resistance such as accessed by CL50 determinations (concentration to reduce 50% of the growth) as while as UFSC-PT24 displayed copper-resistance mechanism at 0.50 mmol L(-1) in solid medium. The UFSC-PT24 and UFSC-Sc124 isolates have increased copper-resistance in liquid medium. The higher production of extracellular pigment was detected in UFSC-Pt116 cultures. The UFSC-Pt116 and UFSC-PT24 isolates showed higher resistance for copper and produced higher mycelium biomass than the other isolates. In this way, the isolates UFSG-Pt116 and UFSC-PT24 can be important candidates to survive in copper-contaminated areas, and can show important role in plants symbiosis in these contaminated sites. PMID:24294261

Silva, R F; Lupatini, M; Trindade, L; Antoniolli, Z I; Steffen, R B; Andreazza, R

2013-01-01

60

Sulfitobacter geojensis sp. nov., Sulfitobacter noctilucae sp. nov., and Sulfitobacter noctilucicola sp. nov., isolated from coastal seawater.  

PubMed

Four Gram-stain-negative, aerobic, rod-shaped bacterial strains, MM-124, MM-126, NB-68 and NB-77, were isolated from the coastal seawater or a region with a bloom of sea sparkle around Geoje island in Korea. The sequence similarity values of the 16S rRNA gene between the isolates and Sulfitobacter mediterraneus DSM 12244(T) ranged from 97.7 to 98.2?%, and phylogenetic relationships suggested that they belong to a phylogenetic branch that includes the genera Sulfitobacter and Roseobacter. The isoprenoid quinone of all three novel strains was ubiquinone-10 and the major fatty acid was cis-vaccenic acid, as in other species of the genus Sulfitobacter. However, there were several differences in the morphological, physiological and biochemical characteristics among the four strains and the reference species of the genus Sulfitobacter. Moreover, the average nucleotide identity values between the three sequenced isolates and the reference strains were below 76.33, indicating that genomic variation exists between the isolates and reference strains. Chemotaxonomic characteristics together with phylogenetic affiliations and genomic distances illustrate that strains MM-124, NB-68 and NB-77 represent novel species of the genus Sulfitobacter, for which the names Sulfitobacter geojensis sp. nov. (type strain MM-124(T)?=?KCTC 32124(T)?=?JCM 18835(T)), Sulfitobacter noctilucae sp. nov. (type strain NB-68(T)?=?KCTC 32122(T)?=?JCM 18833(T)) and Sulfitobacter noctilucicola sp. nov. (type strain NB-77(T)?=?KCTC 32123(T)?=?JCM 18834(T)) are proposed. PMID:25122614

Kwak, Min-Jung; Lee, Jung-Sook; Lee, Keun Chul; Kim, Kwang Kyu; Eom, Mi Kyung; Kim, Byung Kwon; Kim, Jihyun F

2014-11-01

61

Enhanced Endoglucanase Production by Soil Isolates of Fusarium sp. and Aspergillus sp. through Submerged Fermentation Process (Topraktan Batik Kültür Fermentasyonu ile ?zole Edilen Fusarium sp. ve  

Microsoft Academic Search

Objective: The objective was to optimize the fermentation media components and condi- tions to improve the production yield of endoglucanase by filamentous fungi isolated from garden soil. Methods: Cellulolytic fungi were screened from garden soil and identified as Fusarium sp. and Aspergillus sp. by using conidial morphology. The influences of various culture condi- tions including incubation time, temperature, pH, carbon,

Paulchamy Chellapandi; Abha Apurvabhai Jani

62

Biosystems Engineering Catalog 2013 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU  

E-print Network

Biosystems Engineering Catalog 2013 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 28 or Math SAT 630 EF 105

Tennessee, University of

63

Biosystems Engineering Catalog 2014 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU  

E-print Network

Biosystems Engineering Catalog 2014 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 28 or Math SAT 630 EF 105

Grissino-Mayer, Henri D.

64

Nuclear Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP  

E-print Network

Nuclear Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP 16 hours Prereq- Math 130 or math ACT 28 Math 130 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 or Math SAT 630 EF 105

Grissino-Mayer, Henri D.

65

Biomedical Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA,SP,SU Chem 120 or 128 (4) FA, SP, SU  

E-print Network

Biomedical Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA,SP,SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT 28 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 or Math SAT 630 EF 105

Grissino-Mayer, Henri D.

66

Mechanical Engineering Catalog 2014 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU  

E-print Network

Mechanical Engineering Catalog 2014 Fall Math 141 or 147 (4) FA,SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 28 or Math SAT 630 EF 105

Grissino-Mayer, Henri D.

67

Aerospace Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU  

E-print Network

Aerospace Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU 16 hours Prereq- Math 130 or Math ACT 28 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 Math 130 or Math SAT 630 EF 105

Grissino-Mayer, Henri D.

68

Granulicella paludicola gen. nov., sp. nov., Granulicella pectinivorans sp. nov., Granulicella aggregans sp. nov. and Granulicella rosea sp. nov., acidophilic, polymer-degrading acidobacteria from Sphagnum peat bogs.  

PubMed

Five strains of strictly aerobic, heterotrophic bacteria that form pink-red colonies and are capable of hydrolysing pectin, xylan, laminarin, lichenan and starch were isolated from acidic Sphagnum peat bogs and were designated OB1010(T), LCBR1, TPB6011(T), TPB6028(T) and TPO1014(T). Cells of these isolates were Gram-negative, non-motile rods that produced an amorphous extracellular polysaccharide-like substance. Old cultures contained spherical bodies of varying sizes, which represent starvation forms. Cells of all five strains were acidophilic and psychrotolerant, capable of growth at pH 3.0-7.5 (optimum pH 3.8-4.5) and at 2-33°C (optimum 15-22°C). The major fatty acids were iso-C(15?:?0), C(16?:?0) and summed feature 3 (C(16?:?1)?7c and/or iso-C(15?:?0) 2-OH). The major menaquinone detected was MK-8. The pigments were carotenoids. The genomic DNA G+C contents were 57.3-59.3 mol%. The five isolates were found to be members of subdivision 1 of the phylum Acidobacteria and displayed 95.3-98.9?% 16S rRNA gene sequence similarity to each other. The closest described relatives to strains OB1010(T), LCBR1, TPB6011(T), TPB6028(T), and TPO1014(T) were members of the genera Terriglobus (94.6-95.8?% 16S rRNA gene sequence similarity) and Edaphobacter (94.2-95.4?%). Based on differences in cell morphology, phenotypic characteristics and hydrolytic capabilities, we propose a novel genus, Granulicella gen. nov., containing four novel species, Granulicella paludicola sp. nov. with type strain OB1010(T) (=DSM 22464(T) =LMG 25275(T)) and strain LCBR1, Granulicella pectinivorans sp. nov. with type strain TPB6011(T) (=VKM B-2509(T) =DSM 21001(T)), Granulicella rosea sp. nov. with type strain TPO1014(T) (=DSM 18704(T) =ATCC BAA-1396(T)) and Granulicella aggregans sp. nov. with type strain TPB6028(T) (=LMG 25274(T) =VKM B-2571(T)). PMID:20118293

Pankratov, Timofey A; Dedysh, Svetlana N

2010-12-01

69

Amino Alcohols from the Ascidian Pseudodistoma sp  

PubMed Central

Seven new amino alcohol compounds, pseudoaminols A–G (1–7), were isolated from the ascidian Pseudodistoma sp. collected off the coast of Chuja-do, Korea. Structures of these new compounds were determined by analysis of the spectroscopic data and from chemical conversion. The presence of an N-carboxymethyl group in two of the new compounds (6 and 7) is unprecedented among amino alcohols. Several of these compounds exhibited moderate antimicrobial activity and cytotoxicity, as well as weak inhibitory activity toward Na+/K+-ATPase. PMID:24962272

Won, Tae Hyung; You, Minjung; Lee, So-Hyoung; Rho, Boon Jo; Oh, Dong-Chan; Oh, Ki-Bong; Shin, Jongheon

2014-01-01

70

Calcium Carbonate Formation by Synechococcus sp. Strain PCC 8806 and Synechococcus sp. Strain PCC 8807  

SciTech Connect

Precipitation of CaCO3 catalyzed by the growth and physiology of cyanobacteria in the Genus Synechococcus represents a potential mechanism for sequestration of CO2 produced during the burning of coal for power generation. Microcosm experiments were performed in which Synechococcus sp. strain PCC 8806 and Synechococcus sp. strain PCC 8807 were tested for their ability to calcify when exposed to a fixed calcium concentration of 3.4 mM and bicarbonate concentrations of 0.5, 1.25 and 2.5 mM. Disappearance of soluble calcium was used as an indicator of CaCO3 formation; results from metabolically active microcosms were compared to controls with no cells or no carbonate added. Synechococcus sp. strain PCC 8806 removed calcium continuously over the duration of the experiment with approximately 18.6 mg of calcium in the solid phase. Calcium removal occurred over a two-day time period when Synechococcus sp. strain PCC 8807 was tested and only 8.9 mg of calcium was removed in the solid phase. The ability of the cyanobacteria to create an alkaline growth environment appeared to be the primary factor responsible for CaCO3 precipitation in these experiments. Removal of inorganic carbon by fixation into biomass was insignificant compared to the mass of inorganic carbon removed by incorporation into the growing CaCO3 solid.

Lee, Brady D.; William A. Apel; Michelle R. Walton

2006-12-01

71

Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor  

PubMed Central

Background Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. Results 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized. Nucleotide sequences showed 97% and 96% similarity to Methylobacterium sp. AKB-2008-KU9 and Methylobacterium sp. strain 440. The second isolate, likewise, showed 98% similarity to Actinobacterium OR-221. The sequences were deposited in GenBank as Methylobacterium sp. Obi [accession number HQ537130] and Actinobacterium sp. Mayor [accession number JF799090]. Flocculating activity of 95% was obtained in the presence of Ca2+ and heat-stability was exhibited with retention of above 70% activity at 100°C in 30 min. In addition, bioflocculant yield was about 8.203 g/l. A dose of 1 mg/ml of purified bioflocculant was optimal for the clarification of Kaolin suspension (100 ml) following Jar test. FTIR spectrum revealed the presence of carboxyl and hydroxyl functional groups amongst others. Conclusions The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability. PMID:23915393

2013-01-01

72

Nosema tyriae n.sp. and Nosema sp., Microsporidian Parasites of Cinnabar Moth Tyria jacobaeae  

Microsoft Academic Search

Nosema tyriae n.sp. was found in 63% of a population of Cinnabar moth larvae (Tyria jacobaeae). The infection was found in the gut wall, silk glands, and fat body and was probably generalized but appeared to be of low pathogenicity. Merogony and sporogony were by binary fission of diplokaryotic stages. Fresh spores were elongate, slightly pointed at the anterior end,

Elizabeth U Canning; Alan Curry; Sarah A Cheney; Nathalie J Lafranchi-Tristem; Yuji Kawakami; Yoshinori Hatakeyama; Hidetoshi Iwano; Ren Ishihara

1999-01-01

73

Clinical problems of sloths (Bradypus sp. and Choloepus sp.) in captivity.  

PubMed

A 20-yr retrospective study of disease prevalence was carried out for 51 sloths (34 Bradypus sp. and 17 Choloepus sp.) at the São Paulo Zoo. A total of 81 clinical disorders were detected, including nutritional (45.7%), digestive (12.3%), and respiratory (12.3%) problems and injuries (6.1%). A definitive diagnosis was not possible in 8.6% of the cases. The incidence of disease varied according to seasonal climate (winter, 32.5%; spring, 24%; summer, 22.9%; autumn, 20.5%), time in captivity (96.4% of diseases occurred within the first 6 mo and 3.6% occurred thereafter), and type of enclosure (quarantine cage, 96.4%; exhibition enclosure, 3.6%). Both young animals (86.7%) and adults (3.2%) were affected. Parasites were identified by fecal examination in 45.4% of animals with clinical illness (Ascaris sp., 80%; Coccidia sp., 20%). Bacteria such as Salmonella enteritidis, Escherichia coli, and Citrobacter freundii were isolated from feces and/or organs. The first 6 mo in captivity are critical for these animals. Proper management and early identification of medical conditions in captivity have implications for sloth population in the wild. PMID:10367647

Diniz, L S; Oliveira, P M

1999-03-01

74

Conversion and degradation of crude oil by Bacillus SP3  

Microsoft Academic Search

The objective of this study is to demonstrate the basic characteristics of Bacillus SP3 and evaluate its effect on different crude oils. Strain SP3 is a motile, gram-positive, spore-producing rod that was\\u000a isolated from a reservoir of the Shengli oil field in East China. The cells of strain SP3 grew at high temperatures up to\\u000a 58°C at the pH range

Ruixia Hao; Anhuai Lu

2007-01-01

75

Environmental interactions and the SP-100 power system  

NASA Technical Reports Server (NTRS)

Interactions of the SP-100 power system with its expected ambient environments are defined. SP-100 payloads will float 100 V negative of the low Earth orbit (LEO) plasma. Choice of proper geometries and materials will prevent arcing at conductor-insulator junctions in LEO. Care in selecting surface coatings will prevent dielectric breakdown. Sputtering is a concern for long-duration LEO missions. Atomic oxygen durability of SP-100 materials will be tested in ground and flight tests. Evaluation of SP-100 in lunar and planetary environments has begun. The report of a recent workshop on Chemical and Electrical Interactions on Mars identified many of the primary interactions.

Ferguson, Dale C.

1993-01-01

76

IBM SP high-performance networking with a GRF.  

SciTech Connect

Increasing use of highly distributed applications, demand for faster data exchange, and highly parallel applications can push the limits of conventional external networking for IBM SP sites. In technical computing applications we have observed a growing use of a pipeline of hosts and networks collaborating to collect, process, and visualize large amounts of realtime data. The GRF, a high-performance IP switch from Ascend and IBM, is the first backbone network switch to offer a media card that can directly connect to an SP Switch. This enables switch attached hosts in an SP complex to communicate at near SP Switch speeds with other GRF attached hosts and networks.

Navarro, J.P.

1999-05-27

77

Auxiliary-assisted palladium-catalyzed arylation and alkylation of sp2 and sp3 carbon-hydrogen bonds.  

PubMed

We have developed a method for auxiliary-directed, palladium-catalyzed beta-arylation and alkylation of sp(3) and sp(2) C-H bonds in carboxylic acid derivatives. The method employs a carboxylic acid 2-methylthioaniline- or 8-aminoquinoline amide substrate, aryl or alkyl iodide coupling partner, palladium acetate catalyst, and an inorganic base. By employing 2-methylthioaniline auxiliary, selective monoarylation of primary sp(3) C-H bonds can be achieved. If arylation of secondary sp(3) C-H bonds is desired, 8-aminoquinoline auxiliary may be used. For alkylation of sp(3) and sp(2) C-H bonds, 8-aminoquinoline auxiliary affords the best results. Some functional group tolerance is observed and amino- and hydroxy-acid derivatives can be functionalized. Preliminary mechanistic studies have been performed. A palladacycle intermediate has been isolated, characterized by X-ray crystallography, and its reactions have been studied. PMID:20175511

Shabashov, Dmitry; Daugulis, Olafs

2010-03-24

78

Integrative gene cloning and expression system for Streptomyces sp. US 24 and Streptomyces sp. TN 58 bioactive molecule producing strains.  

PubMed

Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch. PMID:19547659

Sioud, Samiha; Aigle, Bertrand; Karray-Rebai, Ines; Smaoui, Slim; Bejar, Samir; Mellouli, Lotfi

2009-01-01

79

Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev.  

PubMed

Two new species of aerobic, gram-negative, peritrichously flagellated or nonmotile marine bacteria usually forming star-shaped aggregates were isolated from northeastern Atlantic Ocean bottom sediments. These organisms resembled eight star-shaped-aggregate-forming bacterial species from the Baltic Sea originally ascribed to the genus Agrobacterium but not included on the Approved Lists of Bacterial Names because of their questionable relationships to true agrobacteria. These two sets of star-shaped-aggregate-forming bacteria were compared by means of phenotypic data, DNA base compositions, DNA-DNA relatedness, and one-dimensional electrophoretic analysis of low-molecular-weight RNAs (5S rRNA and tRNA). According to the results of genotyping, the northeastern Atlantic Ocean isolates and three of the Baltic Sea species formed a group of closely related bacteria that could not be excluded from the genus Agrobacterium with certainty. Until more genotypic data are available, these five marine species are regarded as a distinct subdivision of the genus Agrobacterium consisting of Agrobacterium atlanticum sp. nov. (type strain, 1480T = DSM 5823T), A. meteori sp. nov. (type strain, 1513T = DSM 5824T), A. ferrugineum sp. nov. nom. rev. emend. (type strain, ATCC 25652T), A. gelatinovorum sp. nov. nom. rev. emend. (type strain, ATCC 25655T), and A. stellulatum sp. nov. nom. rev. emend. (type strain, ATCC 15215T). "A. aggregatum" proved to be a later subjective synonym of A. stellulatum, which had priority. The remaining four Baltic Sea species, "A. agile," "A. kieliense," "A. luteum," and "A. sanguineum," could not be placed in the new subdivision of Agrobacterium. PMID:1371058

Rüger, H J; Höfle, M G

1992-01-01

80

LOCALIZATION OF SP22 ON HUMAN SPERM OF DIFFERING QUALITY  

EPA Science Inventory

LOCALIZATION OF SP22 ON HUMAN SPERM OF DIFFERING QUALITY. AE Lavers*1, GR Klinefelter2, DW Hamilton1, KP Roberts1, 1University of Minnesota, Minneapolis, MN and 2US EPA, Research Triangle Park, NC. SP22 is a sperm membrane protein that has been implicated in sperm function d...

81

Complete Genome Sequence of Marinobacter sp. BSs20148.  

PubMed

Marinobacter sp. BSs20148 was isolated from marine sediment collected from the Arctic Ocean at a water depth of 3,800 m. Here we report the complete genome sequence of Marinobacter sp. BSs20148. This genomic information will facilitate the study of the physiological metabolism, ecological roles, and evolution of the Marinobacter species. PMID:23682144

Song, Lai; Ren, Lufeng; Li, Xingang; Yu, Dan; Yu, Yong; Wang, Xumin; Liu, Guiming

2013-01-01

82

Sarcocystis SP. IN THE EASTERN COTTONTAIL (Sylvilagus floridanus)  

Microsoft Academic Search

Sarcocystis sp. was observed in 100 of 185 (54.1%) Eastern cottontails (Sylvilagus floridanus) examined in Pennsylvania over a three year period. Gross and histologic examination commonly revealed cysts in skeletal muscle of the fore and hind legs, flanks and loins. Two rabbits had cysts in esophageal skeletal muscle. Host response to Sarcocystis sp. is described. Adult rabbits had a significantly

M. COSGROVE; J. P. WIGGINS; H. ROTHENBACHER

1982-01-01

83

Nitrogen Control of Atrazine Utilization in Pseudomonas sp. Strain ADP  

Microsoft Academic Search

Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that support rapid growth of Pseudomonas sp. strain ADP compared to cells cultivated

V. Garcia-Gonzalez; Fernando Govantes; Liz J. Shaw; Richard G. Burns; Eduardo Santero

2003-01-01

84

Solar Physics & Space Plasma Research Center (SP2RC)  

E-print Network

Solar Physics & Space Plasma Research Center (SP2RC) University of SheffieldSTFC SSP Intro Summer & Statistics, The University of Sheffield (UK) http://robertus.staff.shef.ac.uk #12;Solar Physics & Space]solitons, applications) ·Conclusions #12;Solar Physics & Space Plasma Research Center (SP2RC) http

85

Analysis of SP-100 critical experiments  

SciTech Connect

In support of the SP-100 space nuclear power source program, preliminary critical benchmark experiments were performed at the ZPPR facility at ANL-W. These configurations are representative of small, fast-spectrum, BeO-reflected, liquid metal-cooled space reactor designs at a 300-kWe power level. Analyses were performed using MCNP (Monte Carlo) and TWODANT (discrete ordinates) transport codes to calculate system criticality, control worth, and power distribution. Both methods calculated eigenvalues within 0.5% of the experimental results. Internal-poison-rod worth was underpredicted and radial reflector worth was overpredicted by both codes by up to 20%. MCNP-calculated control drum worths were underestimated by approximately 8%. Good agreement with experimental values was observed for /sup 235/U fission and for /sup 238/U fission and capture rates with the best agreement occurring in the fuel region and slightly poorer predictions apparent near BeO moderator. 7 refs., 12 figs.

Sapir, J.L.; Brandon, D.I.; Collins, P.J.; Cowan, C.L.; Porter, C.A.; Andre, S.V.

1988-01-01

86

Oxidation of acyclic terpenoids by Corynebacterium sp.  

PubMed Central

Squalene analogs such as lycopersene, geranylfarnesyl, digeranyl, and 2-hydroxy-2,3-dihydrosqualene and terpene alcohol derivatives such as farnesyl benzyl ether, farnesyl pivalate, geranylgeranyl pivalate, geranyl pivalate, and geranyl benzyl ether were oxidized by Corynebacterium sp. strain SY-79, which was isolated from soil by using squalene as a carbon source. Lycopersene and geranylfarnesyl gave no major product. Digeranyl, geranyl benzyl ether, and geranyl pivalate gave terminal oxidation products, and 2-hydroxy-2,3-dihydrosqualene, farnesyl benzyl ether, farnesyl pivalate, and geranylgeranyl pivalate were degraded to give lower molecular carboxylic acids. Strain SY-79 showed promising oxidative activities toward acyclic terpenes, although the metabolites obtained were variable, depending upon the structure of the substrate. PMID:4004225

Yamada, Y; Seo, C W; Okada, H

1985-01-01

87

The Behavior of Heterolepidoderma sp. (Gastrotricha).  

PubMed

The behavior of Heterolepidoderma sp. was studied with the same approach as those already used for many species of ciliates. The ethogram we drew comprehends both helicoidal swimming (n = 20, r = 52.5 +/-12.2 mum, pitch = 512 +/- 101 mum, v--> = 215 +/- 43 mum/sec), periodically interrupted by irregular patterns changing the direction of the swimming of random angles and creeping on the substrate. The latter behavioral state, very common for the species we studied, occurs along tracks formed by successive elements (circular, C, vs linear segments, S) joined to each other by two kinds of reactions, which change their trajectory. The surprising similarities and the unexpected differences between the behavior of this gastrotrich and those of the ciliates already studied from this point of view are discussed, on the basis of the dimensional ranges and ecological niches shared by these two, definitely unrelated groups of organisms. PMID:18429665

Banchetti, R; Nicola, R

1998-01-01

88

Winamp 1.92-SP1  

NSDL National Science Digital Library

Winamp 1.92-SP1, created by Nullsoft, Inc., is a fast, easy-to-use, hi-fidelity music player for Windows 95/98/NT. The player supports numerous audio file formats, most notably MPEG Audio Layer 3 (MP3), which allows near CD-quality sound while compressing a four-minute song into approximately four megabytes. The player has an intuitive interface which includes an equalizer, multi-song programming, and other useful controls. MP3 is becoming widely used as a quality audio compression standard and Winamp is an excellent audio player for use with MP3 and other formats. Winamp is shareware for the Windows 95/98/NT platforms and may be used free of charge for fourteen days, after which time a $10 registration fee is required.

89

SP-100 Control Drive Assembly Development Plan  

NASA Astrophysics Data System (ADS)

The Control Drive Assemblies are the only active components in the entire SP-100 system. Consequently, the design challenges dealing with self-welding, wear, and misalignment in the high temperature and high radiation environment are significant. Because of the harsh environment, it has been necessary to test a variety of materials for such components as bearings, electromagnetic coils, clutches, brakes, and gears. The current Control Drive Assembly technology status is adequate for the 20-KWe design with a five-year life, but has not yet been completely demonstrated for the 100-KWe design with a full ten-year life. The difference in technology status for the 20-KWe and 100-KWe designs is due to the shorter lifetime requirement of the 20-KWe system.

Demuth, Scott F.

1994-07-01

90

Biodegradation of malachite green by Ochrobactrum sp.  

PubMed

This study presents the biodegradation of malachite green (MG), a triphenylmethane dye, using a novel microorganism isolated from textile effluent contaminated environment. The organism responsible for degradation was identified as Ochrobactrum sp JN214485 by 16S rRNA analysis. The effect of operating parameters such as temperature, pH, immobilized bead loading, and initial dye concentration on % degradation was studied, and their optimal values were found to be 30 °C, 6, 20 g/L and 100 mg/L, respectively. The analysis showed that the extracellular enzymes were responsible for the degradation. The biodegradation of MG was confirmed by UV-visible spectroscopic and FTIR analysis. The phytotoxicity test concluded that the degradation products were less toxic compared to MG. The kinetics of biodegradation was studied and the activation energy was found to be 10.65 kcal/mol. PMID:23975693

Vijayalakshmidevi, S R; Muthukumar, Karuppan

2014-02-01

91

Heat stress protection in Aspen sp1 transgenic Arabidopsis thaliana.  

PubMed

It is known that the stable protein 1 (SP1) detected in aspen plants remains soluble upon boiling and that sp1 expression in transgenic aspen is resistant to salt stress. Presently, we analyzed the effect of expression of SP1 in Arabidopsis thaliana plants and their response to high temperature stress. After 45 degrees C for 16 h, relative to wild type plants, sp1 transgenic plants exhibited stronger growth and were better in several physiological properties including chlorophyll, chlorophyll fluorescence, water content, proline content, and malondialdehyde content. These preliminarily results suggest that the over-expression of SP1 may notably enhance heat-tolerant level of transgenic A. thaliana plants. PMID:18510869

Zhu, Bo; Xiong, Ai-Sheng; Peng, Ri-He; Xu, Jing; Zhou, Jun; Xu, Jin-Tao; Jin, Xiao-Fen; Zhang, Yang; Hou, Xi-Lin; Yao, Quan-Hong

2008-05-31

92

Structural and functional aspects of the collectin SP-A.  

PubMed

Surfactant protein A (SP-A), member of the collectin family, is implicated in innate host defense of the lung. SP-A is a "pattern recognition molecule" and interacts with glycoconjugates on the surface of micro-organisms. It protects the lung by interacting with a wide variety of potential pathogens, including viruses, bacteria and fungi. This may result in enhanced killing and clearance by phagocytes. SP-A is a link between the innate and adaptive immune system because it directly affects lymphocyte proliferation and function. Although most extensively studied in the lung, SP-A is found in a number of other sites in the body. The presence of SP-A at these mucosal surfaces, which are in close contact with numerous potentially harmful micro-organisms, supports a more general role for this collectin in mucosal defense. PMID:12396009

Haagsman, Henk P

2002-09-01

93

Carbon-binding designer proteins that discriminate between sp2- and sp3-hybridized carbon surfaces.  

PubMed

Robust and simple strategies to directly functionalize graphene- and diamond-based nanostructures with proteins are of considerable interest for biologically-driven manufacturing, biosensing, and bioimaging. Here, we identify a new set of carbon-binding peptides that vary in overall hydrophobicity and charge and engineer two of these sequences (Car9 and Car15) within the framework of E. coli thioredoxin 1 (TrxA). We develop purification schemes to recover the resulting TrxA derivatives in a soluble form and conduct a detailed analysis of the mechanisms that underpin the interaction of the fusion proteins with carbonaceous surfaces. Although equilibrium quartz crystal microbalance measurements show that TrxA::Car9 and TrxA::Car15 have similar affinities for sp(2)-hybridized graphitic carbon (Kd = 50 and 90 nM, respectively), only the latter protein is capable of dispersing carbon nanotubes. Further investigation by surface plasmon resonance and atomic force microscopy reveals that TrxA::Car15 interacts with sp(2)-bonded carbon through a combination of hydrophobic and ?-? interactions but that TrxA::Car9 exhibits a cooperative mode of binding that relies on a combination of electrostatics and weaker ? stacking. Consequently, we find that TrxA::Car9 binds equally well to sp(2)- and sp(3)-bonded (diamondlike) carbon particles whereas TrxA::Car15 is capable of discriminating between the two carbon allotropes. Our results emphasize the importance of understanding both bulk and molecular recognition events when exploiting the adhesive properties of solid-binding peptides and proteins in technological applications. PMID:23510486

Coyle, Brandon L; Rolandi, Marco; Baneyx, François

2013-04-16

94

Spermatogenesis of riffle bugs, Rhagovelia whitei and Rhagovelia sp (Veliidae), and backswimmers Martarega sp (Notonectidae).  

PubMed

We examined the course of spermatogenesis and the meiotic chromosome complements in aquatic species of true bugs, Heteroptera. The chromosome complement of the Veliidae species was 2n = 39 (38A + X0) and 23 (22A + X0) in Rhagovelia whitei and Rhagovelia sp, respectively, and in the species of the Notonectidae (Martarega sp) it was 26 (22A + 2m + XY); all collected from the region of São José do Rio Preto, SP, Brazil. An impressive characteristic of the first analysis was the size of the cells belonging to Martarega sp, which were six times larger than the same cells in Pentatomidae and twice as large as the cells in aquatic Heteroptera (Gerridae). Regarding spermatogenesis, all the species analyzed showed the same pattern: holocentric chromosomes and elongated spermatids with the chromatin distributed evenly along the head. The family Veliidae showed several bodies impregnated with silver nitrate at prophase, while the family Notonectidae displayed only one. The cells of Notonectidae also showed an evident and round body until the end of prophase I and in the family Veliidae the silver-impregnated bodies were disorganized, where the only region visualized was possibly that of the NOR. In metaphase, silver-stained regions were found at the periphery of all chromosomes in Veliidae and at the periphery of some chromosomes in Notonectidae. The spermatids of Veliidae showed a less silver-impregnated vesicle, while Notonectidae showed silver staining only in part of the nuclear membrane. Therefore, families of Heteroptera have some differences and features that can help identify and classify these species. PMID:22911585

Castanhole, M M U; Pereira, L L V; de Souza, H V; Itoyama, M M

2012-01-01

95

All-carbon sp-sp2 hybrid structures: Geometrical properties, current rectification, and current amplification  

PubMed Central

All-carbon sp-sp2 hybrid structures comprised of a zigzag-edged trigonal graphene (ZTG)and carbon chains are proposed and constructed as nanojunctions. It has been found that such simple hybrid structures possess very intriguing propertiesapp:addword:intriguing. The high-performance rectifying behaviors similar to macroscopic p-n junction diodes, such as a nearly linear positive-bias I-V curve (metallic behavior), a very small leakage current under negative bias (insulating behavior), a rather low threshold voltage, and a large bias region contributed to a rectification, can be predicted. And also, a transistor can be built by such a hybrid structure, which can show an extremely high current amplification. This is because a sp-hybrid carbon chain has a special electronic structure which can limit the electronic resonant tunneling of the ZTG to a unique and favorable situation. These results suggest that these hybrid structures might promise importantly potential applications for developing nano-scale integrated circuits. PMID:23999318

Zhang, Zhenhua; Zhang, Junjun; Kwong, Gordon; Li, Ji; Fan, Zhiqiang; Deng, Xiaoqing; Tang, Guiping

2013-01-01

96

Enzyme-linked imunoassays for the detection of Listeria sp. and Salmonella sp. in sausage: A comparison with conventional methods  

PubMed Central

This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method. PMID:24516447

Benetti, T.M.; Monteiro, C.L.B.; Beux, M.R.; Abrahao, W.M.

2013-01-01

97

Concentration-dependent effects of endogenous S-nitrosoglutathione on gene regulation by specificity proteins Sp3 and Sp1.  

PubMed Central

The activities of certain nuclear regulatory proteins are modified by high concentrations of S-nitrosothiols associated with nitrosative stress. In the present study, we have studied the effect of physiological (low microM) concentrations of the endogenous S-nitrosothiol, GSNO (S-nitrosoglutathione), on the activities of nuclear regulatory proteins Sp3 and Sp1 (specificity proteins 3 and 1). Low concentrations of GSNO increased Sp3 binding, as well as Sp3-dependent transcription of the cystic fibrosis transmembrane conductance regulatory gene, cftr. However, higher GSNO levels prevented Sp3 binding, augmented Sp1 binding and prevented both cftr transcription and CFTR (cystic fibrosis transmembrane conductance regulator) expression. We conclude that low concentrations of GSNO favour Sp3 binding to 'housekeeping' genes such as cftr, whereas nitrosative stress-associated GSNO concentrations shut off Sp3-dependent transcription, possibly to redirect cellular resources. Since low micromolar concentrations of GSNO also increase the maturation and activity of a clinically common CFTR mutant, whereas higher concentrations have the opposite effect, these observations may have implications for dosing of S-nitrosylating agents used in cystic fibrosis clinical trials. PMID:14766015

Zaman, Khalequz; Palmer, Lisa A; Doctor, Allan; Hunt, John F; Gaston, Benjamin

2004-01-01

98

Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used for perchlorate reduction by Azospira sp.  

E-print Network

Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used was shown to be intrinsically different from the enzyme responsible for chlorate and perchlorate [(per)chlo- rate] reduction produced by Azospira sp. KJ based on subunit composition and other enzyme properties

99

Sp1/Sp3 involved in activation of GATA-1 Cell Research | Vol 18 No 2 | February 2008  

E-print Network

that is essential for the terminal maturation of proerythroblasts, megakaryocytic cells and mast cells is restricted to erythroid cells, megakaryocytes, eosinophils and mast cells, as well as to Sertoli cellsSp1/Sp3 involved in activation of GATA-1 302 npg Cell Research | Vol 18 No 2 | February 2008

Tian, Weidong

100

Cross coupling between sp3-carbon and sp3-carbon using a diborylmethane derivative at room temperature.  

PubMed

A novel example of the Suzuki-Miyaura cross-coupling reaction between sp(3)-carbon and sp(3)-carbon is described. The reaction of a diborylmethane derivative with allyl halides or benzyl halides proceeded efficiently in the presence of appropriate Pd-catalysts at room temperature. The present approaches provide functionalized homoallylboronates and alkylboronates with excellent regio- and chemoselectivities. PMID:22540212

Endo, Kohei; Ohkubo, Takahiro; Ishioka, Takafumi; Shibata, Takanori

2012-05-18

101

Wastewater treatment using integrated anaerobic baffled reactor and Bio-rack wetland planted with Phragmites sp. and Typha sp.  

PubMed

The purpose of this study is to examine the potential use of anaerobic baffled reactor (ABR) followed by Bio-rack wetland planted with Phragmites sp. and Typha sp. for treating domestic wastewater generated by small communities (751 mg COD/L, 500 SCOD mg/L, 348 mg BOD5/L). Two parallel laboratory-scale models showed that the process planted with Phragmites sp. and Typha sp. are capable of removing COD by 87% & 86%, SCOD by 90% & 88%, BOD5 by 93% & 92%, TSS by 88% & 86%, TN by 79% & 77%, PO4-P by 21% & 14% at an overall HRT of 21 (843 g COD/m(3)/day & 392 g BOD5/m(3)/day) and 27 (622 g COD/m(3)/day & 302 g BOD5/m(3)/day) hours, respectively. Microbial analysis indicated a high reduction in the MPN of total coliform and TVC as high as 99% at the outlet end of the processes. The vegetated system using Phragmites sp. showed significantly greater (p <0.05) pollutant removal efficiencies due to its extensive root and mass growth rate (p <0.05) of the plant compared to Typha sp. The Phragmites sp. indicated a higher relative growth rate (3.92%) than Typha sp. (0.90%). Microorganisms immobilized on the surface of the Bio-rack media (mean TVC: 2.33?×?10(7) cfu/cm(2)) were isolated, identified and observed using scanning electron microscopy (SEM). This study illustrated that the present integrated processes could be an ideal approach for promoting a sustainable decentralization, however, Phragmites sp. would be more efficient rather than Typha sp. PMID:25379186

Jamshidi, Shervin; Akbarzadeh, Abbas; Woo, Kwang-Sung; Valipour, Alireza

2014-01-01

102

Industrial Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA, SP, SU Chem 120 or 128 (4) FA, SP, SU EF 151 or 157 (4) FA, SP EF 105 (1) FA, SP  

E-print Network

Industrial Engineering Catalog 2014 Fall Math 141 or 147 (4) FA, SP, SU English 101 or 118 (3) FA- Math 130 or Math ACT 28 Math 130 Coreq- Math 141 or 147 and Coreq- EF 151 or 157 or Math SAT 630 EF 105 Spring Math 142 or 148 (4) FA, SP, SU English 102 (3) FA, SP, SU Gen Ed (3) FA, SP, SU EF 152 or 158 (4

Grissino-Mayer, Henri D.

103

Feed training of peacock bass (Cichla sp.).  

PubMed

The Amazonian cichlid peacock bass (Cichla sp.) is a highly marketable food and sport fish, therefore a suitable species for aquaculture. However, because of its piscivorous feeding preferences, the species does not accept dry feeds voluntarily, turning its intensive culture difficult and costly. This study aimed to wean fingerling peacock bass from inert moist food to dry diets. In a first experiment, 1,134 fingerlings weighting 0.27 g were divided in two 0.37 m3 hapas and fed ground fish flesh with 35% success. Then, 1.3 g fish were pooled, stocked in six 25 L cages and fed two pellet sequences with 80%, 60%, 40%, 20% and 0% ground fish flesh (GFF). One sequence was flavored with 10% krill meal (Euphausia sp.). Training success of fish fed the GFF-00 diet flavored with krill reached 12%a compared to 11.6%a (p < 0.05) for diets without krill meal. A second experiment was set up with 969, 1.5 g fish, trained with GFF with 39.8% success. After the feed training period, 2.2 g fish were then fed a sequence of moist pellets containing 80%, 60% and 45% GFF. Fish trained to feed on moist pellets with 45% ground fish were pooled and stocked into nine 25 L cages. Fish were weaned to dry pellets without ground fish flesh (GFF-00) using three diet sequences: 1) dry pellets; 2) moist pellets; and 3) dry pellets flavored with 4% cod liver oil; all three diets contained 30, 10 and 0% GFF. The three sequences yielded, respectively 30.8%a, 23.6%a, and 24.7%a (p < 0.05) fish feeding on GFF-00. There were no apparent beneficial effects of increasing moisture or addition of cod liver oil as flavor enhancers in the weaning diets. This study revealed the feasibility of training peacock bass to accept dry pellets, but feeding young fish ground fish flesh seemed to be a major bottleneck in improving feed training success. PMID:11241964

Moura, M A; Kubitza, F; Cyrino, J E

2000-11-01

104

Possible ferromagnetism in s- and sp-electron element nanowires  

NASA Astrophysics Data System (ADS)

We present a general physical picture for the one-dimensional nanomagnetism of elements with only s- or sp-valence electrons based on the atomic orbital interactions, first-principles calculations and Stoner criterion combined. It is shown that s- and sp-electron atoms, such as Na, Mg, Al, and Si, can exhibit magnetism in the geometry of linear chains. The s-electron elements show magnetism when the bonds are compressed, and, in contrast, the sp-elements exhibit magnetism when the bonds are elongated. The underlying mechanism has been understood by a general physical picture, that is, the exchanges among electronic states of multiple subbands around the Fermi level.

Zhu, Zi-Zhong; Zheng, Jin-Cheng; Guo, Guang-Yu

2009-04-01

105

A review of the OvidSP platform.  

PubMed

As part of its mission to advance the technological capabilities of its products, Ovid introduced a redesign of its database platform in fall 2007. The platform redesign brought new features and enhancements to an already robust system. The transition between Ovid Gateway and the OvidSP platform was orchestrated seamlessly. With the OvidSP platform came innovative changes such as the incorporation of Natural Language Processing associated with the new Basic Search mode. Discussed are OvidSP features, advantages and disadvantages of the new platform, customizable settings, and future direction. PMID:19042719

FitzSimmons, Marie; Gross, Valerie A

2008-01-01

106

Microbial transformation of cephalomannine by Luteibacter sp.  

PubMed

Luteibacter sp., a new bacterium isolated from the soil around a Taxus cuspidata Sieb. et Zucc plant, was studied for its capability to metabolize cephalomannine (1). After preparative fermentation, eight metabolites were obtained and characterized as baccatin III (2), baccatin V (3), 10-deacetylbaccatin III (4), 10-deacetyl-10-oxobaccatin V (5), 7-epicephalomannine (6), 10-deacetylcephalomannine (7), 10-deacetyl-7-epicephalomannine (8), and 3'-N-debenzoyl-3'-N-(2-methylbutyryl)-7-epitaxol (9). Among these metabolites, 9 is a new compound. Epimerization of the 7beta-OH group and hydrolysis of the C-13 side-chain were the two major reactions in this bioprocess. However, the biotransformation of 7beta-D-xylosyl-10-deacetyltaxol (10) with the same strain yielded a C-13 side-chain eliminated product without epimerization at C-7 (11). Metabolites 5-9 and 11, together with 1 and paclitaxel, were evaluated for their inhibitory activities against five human cancer cell lines (HCT-8, Bel-7402, BGC-823, A549, and A2780). All these compounds showed less potent activities than paclitaxel, which is currently used in clinical chemotherapy. PMID:18001087

Li, Jianhua; Dai, Jungui; Chen, Xiaoguang; Zhu, Ping

2007-12-01

107

Vibrio cortegadensis sp. nov., isolated from clams.  

PubMed

A group of four strains isolated from clams (Venerupis decussata and Venerupis philippinarum) in Galicia (NW Spain) were subjected to a polyphasic characterization, based on the phenotypic characteristics, the analysis of chemotaxonomic features, the sequencing of the 16S rRNA and five housekeeping (atpA, pyrH, recA, rpoA and rpoD) genes, as well as DNA-DNA hybridization (DDH). The analysis of the phenotypic and chemotaxonomic characteristics and the results of a phylogenetic study, based on the 16S rRNA gene sequence analysis and multilocus sequence analysis, clearly indicated that these strains belong to the genus Vibrio and were allocated between the Splendidus and Anguillarum clades showing a close relationship with the type strains of Vibrio tapetis (98.8 %), Vibrio pomeroyi (98.0 %) and Vibrio crassostreae (97.9 %). DNA-DNA hybridization results confirmed that these isolates constitute a new species. The name Vibrio cortegadensis sp. nov. is proposed with C 16.17(T) (=CECT 7227(T)=LMG 27474(T)) as the type strain. PMID:24271473

Lasa, Aide; Diéguez, Ana L; Romalde, Jesús L

2014-02-01

108

Blastocystis sp. from food animals in India.  

PubMed

Blastocystis, a zoonotic protozoan found in the intestinal tracts of a wide range of animals, has not been reported from non-human hosts from India so far. Organisms indistinguishable from Blastocystis sp. were identified in the Giemsa stained intestinal scrapings collected from carcasses of piglet and poultry that were brought for necropsy to the Central University Laboratory, Chennai. The 'central vacuole forms' of the parasite, with number of nuclei ranging from 1 to 12 were identified. The intensity of infection was low, with less than one organism per oil immersion field, indicating that their presence was unconnected to the cause of death. Caecal scraping was found to be more ideal than duodenal scraping for the diagnosis of Blastocystis, and can be a potential specimen for definitive diagnosis. Identical organisms were also detected in the dung samples of a buffalo calf which showed clinical signs of diarrhoea The presence of Blastocystis in food animals acquires public health significance, as many subtypes of the parasite from poultry and pigs are transmissible to humans. PMID:25320500

Sreekumar, C; Selvaraj, J; Gomathinayagam, S; Thangapandiyan, M; Ravikumar, G; Roy, Parimal; Balachandran, C

2014-12-01

109

[Keratinolytic activity of Streptomyces sp. 1382].  

PubMed

Research in the growth dynamics of Streptomyces sp. 1382--a producer of keratinolytic enzyme--has shown that maximum of its biosynthesis in the culture medium is achieved on the 5th day, that correlates with maximum of biomass accumulation. The enzyme complex, obtained from culture liquid, is active in the wide range of pH (4-11) and temperature (10-70 degrees C). Under such conditions it is stable and does not lose its activity during several hours. The preparation possesses different pH-optima of keratinase and total proteolytic activities: in neutral (pH 7.0) and weakly alkali (pH 8.0) regions, as well as different thermal optima at 37-40 and 50-60 degrees C, respectively. Four types of colonies whose level of keratinase activity was considerably different have been found, when analyzing heterogeneity of the strain-producer population. Study of the effect of various sources of carbon and nitrogen on proteases synthesis by the culture has found the activating effect of arabinose, maltose, peptone and yeast autolysate. Under such conditions synthesis of keratinolytic enzyme increased 6-7 time, only the sheep wool and chicken feather being added as the only source of carbon and nitrogen. The induction with substrate is discussed as a possible mechanism of keratinase synthesis regulation. PMID:15104049

Ivanko, O V; Varbanets, L D

2004-01-01

110

Preliminary SP-100/Stirling heat exchanger designs  

NASA Technical Reports Server (NTRS)

Analytic modeling of several heat exchanger concepts to couple the SP-100 nuclear reactor primary lithium loop and the Space Stirling Power Convertor (SSPC) was performed. Four 25 kWe SSPC's are used to produce the required 100 kW of electrical power. This design work focused on the interface between a single SSPC and the primary lithium loop. Manifolding to separate and collect the four channel flow was not modeled. This work modeled two separate types of heat exchanger interfaces (conductive coupling and radiative coupling) to explore their relative advantages and disadvantages. The minimum mass design of the conductively coupled concepts was 18 kg or 0.73 kg/kWe for a single 25 kWe convertor. The minimum mass radiatively coupled concept was 41 kg or 1.64 kg/kWe. The direct conduction heat exchanger provides a lighter weight system because of its ability to operate the Stirling convertor evaporator at higher heat fluxes than those attainable by the radiatively coupled systems. Additionally the conductively coupled concepts had relatively small volumes and provide potentially simpler assembly. Their disadvantages were the tight tolerances and material joining problems associated with this refractory to superalloy interface. The advantages of the radiatively coupled designs were the minimal material interface problems.

Schmitz, Paul; Tower, Leonard; Dawson, Ronald; Blue, Brian; Dunn, Pat

1993-01-01

111

Preliminary SP-100/Stirling heat exchanger designs  

SciTech Connect

Analytic modeling of several heat exchanger concepts to couple the SP-100 nuclear reactor lithium loop and the Space Stirling Power Convertor (SSPC) was performed. Four 25 kWe SSPC`s are used to produce the required 100 kW of electrical power. This design work focused on the interface between a single SSPC and the primary lithium loop. Manifolding to separate and collect the four channel flow was not modeled. This work modeled two separate types of heat exchanger interfaces (conductive coupling and radiative coupling) to explore their relative advantages and disadvantages. The minimum mass design of the conductively coupled concepts was 18 kg or 0.73 kg/kWe for a single 25 kWe convertor. The minimum mass radiatively coupled concept was 41 kg or 1.64 kg/kWe. The direct conduction heat exchanger provides a lighter weight system because of its ability to operate the Stirling convertor evaporator at higher heat fluxes than those attainable by the radiatively coupled systems. Additionally the conductively coupled concepts had relatively small volumes and provide potentially simpler assembly. Their disadvantages were the tight tolerances and material joining problems associated with this refractory to superalloy interface. The advantages of the radiatively coupled designs were the minimal material interface problems.

Schmitz, P.; Tower, L. [Sverdrup Technology, Inc., Brook Park, OH (United States). Lewis Research Center Group; Dawson, R. [Aerospace Design and Fabrication Inc., Brook Park, OH (United States); Blue, B.; Dunn, P. [National Aeronautics and Space Administration, Cleveland, OH (United States). Lewis Research Center

1994-09-01

112

Lactobacillus taiwanensis sp. nov., isolated from silage.  

PubMed

Strain FIRDI 006T, isolated from silage cattle feed, was characterized by using phenotypic and molecular taxonomic methods. The strain was a homofermentative lactic acid bacterium; the cells stained Gram-positive and were catalase-negative, non-motile, facultatively anaerobic rods. 16S rRNA gene sequence analysis revealed that the strain belongs phylogenetically to the genus Lactobacillus and can be placed within the Lactobacillus acidophilus-delbrueckii group. Lactobacillus gasseri and Lactobacillus johnsonii were the most closely related species, with 16S rRNA gene sequence similarities of 99.53 and 99.46% to the respective type strains. Low gyrB gene sequence similarities (<90%) and low DNA-DNA reassociation values (<45%) were obtained between the strain and the phylogenetically closest neighbours. Based on phenotypic and genetic evidence, the strain is considered to represent a novel species, for which the name Lactobacillus taiwanensis sp. nov. is proposed. The type strain is FIRDI 006T (=BCRC 17755T =DSM 21401T). PMID:19605711

Wang, Li-Ting; Kuo, Hsiao-Ping; Wu, Yen-Chi; Tai, Chun-Ju; Lee, Fwu-Ling

2009-08-01

113

Description of Ectothiorhodospira salini sp. nov.  

PubMed

Strain JA430(T) is a Gram-negative, vibrioid to spiral shaped phototrophic purple sulfur bacterium isolated from anoxic sediment of a saltern at Kanyakumari in a mineral salts medium that contained 2% NaCl (w/v). Strain JA430(T) grows optimally at 5-6% NaCl and tolerates up to 12% NaCl. Intracellular photosynthetic membranes were of the lamellar type. Bacteriochlorophyll a and carotenoids of the spirilloxanthin series are present as photosynthetic pigments. Major cellular fatty acids are C(18:1)?7c, C(16:0), C(19:0)cyclo?8c and C(16:1)?7c/C(16:1)?6c. Strain JA430(T) exhibits photoorganoheterotrophy and chemoorganoheterotrophy and requires para-aminobenzoic acid, pantothenate and pyridoxal phosphate for growth. Phylogenetic analysis on the basis of 16S rRNA gene sequence analysis showed that strain JA430(T) forms monophyletic group in the genus Ectothiorhodospira. The highest sequence similarity for strain JA430(T) was found with the type strains of Ectothiorhodospira variabilis DSM 21381(T) (96.1%) and Ectothiorhodospira haloalkaliphila ATCC 51935(T) (96.2%). Morphological and physiological characteristics discriminate strain JA430(T) from other species of the genus Ectothiorhodospira, for which we describe this as a novel species, Ectothiorhodospira salini sp. nov. ( = NBRC 105915(T) = KCTC 5805(T)). PMID:20953095

Venkata Ramana, Vemuluri; Sasikala, Chintalapati; Veera Venkata Ramaprasad, Eedara; Venkata Ramana, Chintalapati

2010-08-01

114

Actinomadura rudentiformis sp. nov., isolated from soil.  

PubMed

As part of an antibiotic-screening programme, the actinomycete strain HMC1(T) was isolated from soil collected from the banks of the Gamka River in the Swartberg Nature Reserve, Western Cape Province, South Africa. The isolate produced branching vegetative mycelia, which entwined to form rope-like structures. It exhibited antibiosis against Enterococcus faecium VanA (a vancomycin-resistant clinical strain) and Mycobacterium aurum A+. Organic solvent extracts of the culture filtrate and mycelial mass of strain HMC1(T) exhibited moderate antibiosis against Mycobacterium aurum A+, Mycobacterium bovis BCG (Tokyo) and Mycobacterium tuberculosis H37Rv(T). The chemotaxonomic characteristics of strain HMC1(T) corresponded with those of members of the genus Actinomadura. Furthermore, phylogenetic analysis based on the 16S rRNA gene showed that the strain was closely related to members of the genus Actinomadura. Phenotypic and 16S rRNA gene sequence analyses indicated that strain HMC1(T) represents a novel species of the genus Actinomadura, for which the name Actinomadura rudentiformis sp. nov. is proposed. The type strain is HMC1(T) (=DSM 44962(T)=NRRL B-24458(T)). PMID:17220439

le Roes, Marilize; Meyers, Paul R

2007-01-01

115

Planobispora takensis sp. nov., isolated from soil.  

PubMed

A novel Gram-stain-positive, filamentous bacterial strain (A-T 7458(T)) was isolated from soil collected from hill evergreen forest in Thailand. The strain developed cylindrical sporangia containing a longitudinal pair of motile spores, on short ramifications of aerial mycelia. From a taxonomic study using a polyphasic approach, strain A-T 7458(T) had typical characteristics of members of the genus Planobispora. 16S rRNA gene sequence analysis indicated that the isolate was closely related to Planobispora siamensis A-T 4600(T) (98.5?%), Planobispora rosea JCM 3166(T) (97.6?%) and Planobispora longispora NBRC 13918(T) (97.6?%). The DNA-DNA relatedness values, which differentiated the novel strain from the closest species, were significantly below 70?%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinone was MK-9(H2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17?:?1 and C18?:?1, and saturated C16?:?0 and C17?:?0. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the novel isolate is proposed to represent a novel species of the genus Planobispora, to be named Planobispora takensis sp. nov. The type strain is A-T 7458(T) (?=?BCC 48396(T)?=?NBRC 109077(T)). PMID:24408527

Ngaemthao, Wipaporn; Suriyachadkun, Chanwit; Chunhametha, Suwanee; Niemhom, Nantawan; Thawai, Chitti; Sanglier, Jean-Jacques

2014-04-01

116

Planobispora siamensis sp. nov., isolated from soil.  

PubMed

A novel actinomycete strain, A-T 4600(T), which developed cylindrical sporangia containing a longitudinal pair of motile spores forming singly or in bundles on short ramifications of the aerial mycelium, was isolated from soil collected from an evergreen forest in Thailand. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinones were MK-9(H2). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannoside, diphosphatidylglycerol and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17 : 1, C18 : 1, saturated C16 : 0, and C17 : 0. The G+C content of the DNA was 70.8 mol%. Phenotypic and chemotaxonomic analyses showed that the isolate had the typical characteristics of members of the genus Planobispora. Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belonged to the genus Planobispora but as a putative novel species. DNA-DNA relatedness values that differentiate the isolate from previously described members of the genus Planobispora were significantly below 70 %. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, it is proposed that the isolate represents a novel species, Planobispora siamensis sp. nov.; the type strain is A-T 4600(T) ( = BCC 39469(T) = NBRC 107568(T)). PMID:23291889

Ngaemthao, Wipaporn; Suriyachadkun, Chanwit; Chunhametha, Suwanee; Tamura, Tomohiko; Sanglier, Jean-Jacques

2013-07-01

117

Mucilaginibacter flavus sp. nov., isolated from wetland.  

PubMed

A non-motile, pale yellow, colony-forming strain, designated HME6839(T), was isolated from the wetland of Jeju Island, Republic of Korea. The major fatty acids of strain HME6839(T) were summed feature 3 (comprising C16?:?1?6c and/or C16?:?1?7c), iso-C15?:?0 and C16?:?1?5c. The DNA G+C content was 41.2 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME6839(T) formed a lineage within the genus Mucilaginibacter. Strain HME6839 (T) [corrected] was closely related to Mucilaginibacter dorajii (96.7?%), Mucilaginibacter polysacchareus (96.5?%) and Mucilaginibacter lappiensis (96.3?%). On the basis of the chemotaxonomic and phylogenetic results presented in this study, strain HME6839(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter flavus sp. nov., is proposed. The type strain is HME6839(T) (?=?KCTC 23441(T)?=?CECT 7857(T)). PMID:24425737

Joung, Yochan; Kim, Haneul; Lee, Beom-Il; Kang, Heeyoung; Kim, Tae-Su; Kim, Seung Bum; Joh, Kiseong

2014-04-01

118

Sp1 and Sp3 mediate basal and serum-induced expression of human CENP-W.  

PubMed

Cancer-upregulated gene 2 (CUG2), which was named since it was originally identified as a putative oncogene up-regulated in various human cancers, was recently renamed CENP-W based on the new findings that it is a component of the centromeric complex playing a crucial role in the assembly of functional kinetochore complex during mitosis. To understand the transcriptional regulation of CENP-W, we analyzed its TATA-less promoter and identified a GC-rich putative Sp1 binding site located at -46 to -36 that was critical in CENP-W expression. Competitive electrophoretic gel mobility shift assay using mutated oligos and supershift assays with Sp1 and Sp3 antibodies demonstrated that both proteins specifically bound to this promoter region. Moreover, we found that CENP-W was highly induced by serum stimulation followed by serum deprivation, with Sp1 and Sp3 transcription factors involved in this transactivation. Taken together, our results suggest that Sp1 together with Sp3 may function as the main regulator of the basal and serum-induced transcription of CENP-W. PMID:20180024

Kim, Hyejin; Lee, Sunhee; Park, Byoungwoo; Che, Lihua; Lee, Soojin

2010-10-01

119

'PROCTOECES' SP. (TREMATODA: DIGENEA) IN THE AMERICAN OYSTER, 'CRASSOSTREA VIRGINICA'  

EPA Science Inventory

Histological examination of over 6,000 oysters, Crassostrea virginica, inhabiting northern Gulf Coast estuaries revealed unencysted junvenile and possible adult stages of digenetic trematode, Proctoeces sp., inhabiting the gonadal ducts of the mollusc. The morphology of the worm ...

120

Telosticta iban sp. nov. from Sarawak (Odonata: Zygoptera: Platystictidae) .  

PubMed

T. iban sp. nov. is described from the Lanjak Entimau Wildlife Sanctuary in Sarawak, Malaysian Borneo. Both sexes can be distinguished from all other species of Telosticta by the form of the antehumeral markings. PMID:24872033

Dow, Rory A

2014-01-01

121

Stimulation of bioluminescence in Noctiluca sp. using controlled temperature changes.  

PubMed

Bioluminescence induced by multifarious stimuli has long been observed and is remains under investigation because of its great complexity. In particular, the exact mechanism underlying bioluminescence is not yet fully understood. This work presents a new experimental method for studying Noctiluca sp. bioluminescence under temperature change stimulation. It is a study of Noctiluca sp. bioluminescence using controlled temperature changes in a tank. A characteristic of this experiment is the large volume of water used (1?m(3) in a tank of 2?×?1?×?1?m). Temperature changes were controlled by two methods. In the first, a flask filled with hot water was introduced into the tank and in the second, a water heater was used in the tank. Temperature changes were recorded using sensors. Noctiluca sp. bioluminescence was recorded using a Canon 5D Mark II and this allowed the characteristics of Noctiluca sp. bioluminescence under temperature change stimulation to be monitored. PMID:23001957

Han, Jing; Li, GuiJuan; Liu, HuanYing; Hu, HaoHao; Zhang, XueGang

2013-01-01

122

Characterization of Pseudomonas sp. BCNU 171 tolerant to organic solvents.  

PubMed

An organic solvent-tolerant bacterium, designated as Pseudomonas sp. BCNU 171, was isolated from industrial wastewater in Korea, on the basis of its ability to survive in the presence of benzene, toluene, propylbenzene and xylenes. Its tolerance limits were 8 mM in phenol, 20 mM in benzene and 60 M in toluene. The log P value of phenol was approximately 1.5, which indicates that Pseudomonas sp. BCNU 171 exhibits the highest tolerance to organic solvents. Pseudomonas sp. BCNU 171, a relative of P. putida, P. mosselii and P. moteillii based on phylogenetic analyses using 16S rRNA sequences, was designated as a new sp. that is tolerant to a wide spectrum of organic solvents, especially xylene isomers. These findings may facilitate the understanding of organic solvent tolerance in bacterial cells. PMID:18792055

Choi, Hye Jung; Kim, Seon-A; Kim, Dong Wan; Moon, Ja Young; Jeong, Yong Kee; Joo, Woo Hong

2008-12-01

123

ORIGINAL PAPER Ambrosiella beaveri, sp. nov., Associated with an exotic  

E-print Network

ORIGINAL PAPER Ambrosiella beaveri, sp. nov., Associated with an exotic ambrosia beetle was introduced along with the beetle into North America. Keywords Ambrosia fungi Á Candida homelintoma Á Geosmithia lavendula Á Geosmithia obscura Á Scolytinae Introduction Ambrosia beetles (Coleoptera

124

Les ganodermes (Ganoderma sp.) Ganoderma adspersum (Schulz.) Donk  

E-print Network

Les ganodermes (Ganoderma sp.) Ganoderma adspersum (Schulz.) Donk Ordre : Ganodermales Famille conjonctives brunes à parois épaisses, r P induit une pou #12;Le ganoderme aplani Ganoderma lipsiense (Batsch

Chamroukhi, Faicel

125

Untersuchungen zum Acarbose-Metabolismus von Actinoplanes sp.  

E-print Network

??Acarbose hat als Inhibitor von Hydrolasen alpha-1,4-glykosidischer Bindungen medizinische Bedeutung. Das Acarbose-Biosynthese-Gencluster (acb) des grampositiven Produzenten Actinoplanes sp. wurde identifiziert und Genprodukte z. T. charakterisiert.… (more)

Brunkhorst, Claudia

2005-01-01

126

Geobacter uraniireducens sp. nov., isolated from subsurface sediment undergoing uranium  

E-print Network

Geobacter uraniireducens sp. nov., isolated from subsurface sediment undergoing uranium with acetate oxidation, was isolated from subsurface sediment undergoing uranium bioremediation. The 16S r was to recover an environmentally relevant Geobacter strain from subsurface sediments undergoing in situ uranium

Lovley, Derek

127

Isolation and identification of a toxic metabolite of Phomopsis sp.  

E-print Network

MAY 1982 Major Subject~ Veterinary Toxicology ISOLATION AND IDENTIFICATION OF A TOXIC METABOLITE OF PHOMOPSIS SP. A Thesis by DANIEL ROBERT SAMPLES Approved as to style and content by: (Chairman of Committee) (Membe (Mem ) (Head of Departme t... MAY 1982 Major Subject~ Veterinary Toxicology ISOLATION AND IDENTIFICATION OF A TOXIC METABOLITE OF PHOMOPSIS SP. A Thesis by DANIEL ROBERT SAMPLES Approved as to style and content by: (Chairman of Committee) (Membe (Mem ) (Head of Departme t...

Samples, Daniel Robert

2012-06-07

128

[Causative agent of human diphyllobothriasis--Diphyllobothrium klebanovskii sp. n].  

PubMed

A new cestode species, Diphyllobothrium klebanovskii sp. n., is described on the basis of experimental examination and morphological study of 51 specimens expelled from men in lower part of the Amur river and 18 specimens expelled from golden hamsters. The larval stage of this cestode is plerocercoid of type F, which is localized in muscles of Oncorhynchus keta and O. gorbuscha. D. klebanovskii sp. n. is a marine species, it differs from D. latum and other species of the genus Diphyllobothrium. PMID:3387122

Muratov, I V; Posokhov, P S

1988-01-01

129

2-Hydroxyisonicotinate dehydrogenase isolated from Mycobacterium sp. INA1  

Microsoft Academic Search

2-Hydroxyisonicotinate dehydrogenase from Mycobacterium sp. INA1 was purified 26-fold to apparent homogeneity. The enzyme is involved in isonicotinate degradation by Mycobacterium sp. INA1 and catalyzes the conversion of 2-hydroxyisonicotinate to 2,6-dihydroxypyridine-4-carboxylate. The purified protein exhibited a native molecular mass of 300 kDa and subunits of 97, 31 and 17 kDa, respectively, indicating an ?2?2?2 structure. The absorption spectrum of the

Thomas Schräder; Cornelia Hillebrand; Jan R Andreesen

1998-01-01

130

Sarcocystis sp. in the eastern cottontail (Sylvilagus floridanus).  

PubMed

Sarcocystis sp. was observed in 100 of 185 (54.1%) Eastern cottontails (Sylvilagus floridanus) examined in Pennsylvania over a three year period. Gross and histologic examination commonly revealed cysts in skeletal muscle of the fore and hind legs, flanks and loins. Two rabbits had cysts in esophageal skeletal muscle. Host response to Sarcocystis sp. is described. Adult rabbits had a significantly greater rate of infection (69.3%) than juveniles (20.7%) (P less than 0.01). PMID:6808158

Cosgrove, M; Wiggins, J P; Rothenbacher, H

1982-01-01

131

Methods for the genetic manipulation of Nonomuraea sp. ATCC 39727  

Microsoft Academic Search

Nonomuraea sp. ATCC 39727 belongs to the Streptosporangiaceae family of filamentous actinomycetes. This microorganism produces the teicoplanin-like glycopeptide A40926, which is the starting\\u000a material for the synthesis of the second-generation glycopeptide dalbavancin. Notwithstanding the strain’s pharmaceutical\\u000a relevance, the lack or poor efficiency of genetic tools to manipulate Nonomuraea sp. ATCC 39727 has hampered strain and product improvement. Here we report

Giorgia Letizia Marcone; Lucy Foulston; Elisa Binda; Flavia Marinelli; Mervyn Bibb; Fabrizio Beltrametti

2010-01-01

132

Environmental interactions and the SP-100 power system  

SciTech Connect

The Civil Space Technology Initiative High-Capacity-Power Environmental Interactions Program has made great progress in defining and evaluating the interactions of the SP-100 power system with its expected ambient environments. The NASCAP/LEO and POLAR computer codes demonstrated that local electric fields at the user interface module are high. Therefore, particular attention must be paid to geometries and materials in this region to prevent arcing at conductor-insulator junctions in low Earth orbit. NASCAP/LEO and EPSAT computer models revealed that SP-100 payloads float about 100 V negative of the LEO plasma. In addition, ground tests and modeling done for the Space Station Freedom Electrical Grounding Tiger Team found that dielectric coatings often break down at such voltages in a plasma. Thus, surface coatings for SP-100 payloads should be carefully selected. Sputtering may also be a concern for long-duration missions in LEO at these voltages. Much work has been done on a sputtering model to evaluate surface material loss rates on SP-100 payloads. In ground plasma chamber tests of cables and cable insulators at SP-100 voltages, parasitic power losses due to the plasma current collected from possible pinholes or coating defects were quantified and shown to be small. Modeling revealed that the power loss from currents to other surfaces is also small. The atomic oxygen durability of SP-100 materials and coatings continues to be investigated in ground tests. In the upcoming Evaluation of Oxygen Interaction with Materials (EOIM-3) Shuttle flight experiment, a host of SP-100 materials will be evaluated for atomic oxygen durability in LEO. Finally, an evaluation of the interactions of the SP-100 power system with lunar and planetary environments has started. At a workshop on chemical and electrical interactions on Mars recently held at the NASA Lewis Research Center, many of primary interactions were identified.

Ferguson, D.C.

1993-01-01

133

SP8 regulates signaling centers during craniofacial development  

PubMed Central

Much of the bone, cartilage and smooth muscle of the vertebrate face is derived from neural crest (NC) cells. During craniofacial development, the anterior neural ridge (ANR) and olfactory pit (OP) signaling centers are responsible for driving the outgrowth, survival, and differentiation of NC populated facial prominences, primarily via FGF. While much is known about the functional importance of signaling centers, relatively little is understood of how these signaling centers are made and maintained. In this report we describe a dramatic craniofacial malformation in mice mutant for the zinc finger transcription factor gene Sp8. At E14.5 they show facial prominences that are reduced in size and underdeveloped, giving an almost faceless phenotype. At later times they show severe midline defects, excencephaly, hyperterlorism, cleft palate, and a striking loss of many NC and paraxial mesoderm derived cranial bones. Sp8 expression was primarily restricted to the ANR and OP regions during craniofacial development. Analysis of an extensive series of conditional Sp8 mutants confirmed the critical role of Sp8 in signaling centers, and not directly in the NC and paraxial mesoderm cells. The NC cells of the Sp8 mutants showed increased levels of apoptosis and decreased cell proliferation, thereby explaining the reduced sizes of the facial prominences. Perturbed gene expression in the Sp8 mutants was examined by laser capture microdissection coupled with microarrays, as well as in situ hybridization and immunostaining. The most dramatic differences included striking reductions in Fgf8 and Fgf17 expression in the ANR and OP signaling centers. We were also able to achieve genetic and pharmaceutical partial rescue of the Sp8 mutant phenotype by reducing Sonic Hedgehog (SHH) signaling. These results show that Sp8 primarily functions to promote Fgf expression in the ANR and OP signaling centers that drive the survival, proliferation, and differentiation of the NC and paraxial mesoderm that make the face. PMID:23872235

Kasberg, Abigail D.; Brunskill, Eric W.; Potter, S. Steven

2014-01-01

134

Morphologic and molecular characterization of new Cyclospora species from Ethiopian monkeys: C. cercopitheci sp.n., C. colobi sp.n., and C. papionis sp.n.  

PubMed Central

In recent years, human cyclosporiasis has emerged as an important infection, with large outbreaks in the United States and Canada. Understanding the biology and epidemiology of Cyclospora has been difficult and slow and has been complicated by not knowing the pathogen s origins, animal reservoirs (if any), and relationship to other coccidian parasites. This report provides morphologic and molecular characterization of three parasites isolated from primates and names each isolate: Cyclospora cercopitheci sp.n. for a species recovered from green monkeys, C. colobi sp.n. for a parasite from colobus monkeys, and C. papionis sp.n. for a species infecting baboons. These species, plus C. cayetanensis, which infects humans, increase to four the recognized species of Cyclospora infecting primates. These four species group homogeneously as a single branch intermediate between avian and mammalian Eimeria. Results of our analysis contribute toward clarification of the taxonomic position of Cyclospora and its relationship to other coccidian parasites. PMID:10511521

Eberhard, M. L.; da Silva, A. J.; Lilley, B. G.; Pieniazek, N. J.

1999-01-01

135

Acinetobacter kookii sp. nov., isolated from soil.  

PubMed

Two Gram-stain-negative, non-fermentative bacterial strains, designated 11-0202(T) and 11-0607, were isolated from soil in South Korea, and four others, LUH 13522, LUH 8638, LUH 10268 and LUH 10288, were isolated from a beet field in Germany, soil in the Netherlands, and sediment of integrated fish farms in Malaysia and Thailand, respectively. Based on 16S rRNA, rpoB and gyrB gene sequences, they are considered to represent a novel species of the genus Acinetobacter. Their 16S rRNA gene sequences showed greatest pairwise similarity to Acinetobacter beijerinckii NIPH 838(T) (97.9-98.4?%). They shared highest rpoB and gyrB gene sequence similarity with Acinetobacter johnsonii DSM 6963(T) and Acinetobacter bouvetii 4B02(T) (85.4-87.6 and 78.1-82.7?%, respectively). Strain 11-0202(T) displayed low DNA-DNA reassociation values (<40?%) with the most closely related species of the genus Acinetobacter. The six strains utilized azelate, 2,3-butanediol, ethanol and dl-lactate as sole carbon sources. Cellular fatty acid analyses showed similarities to profiles of related species of the genus Acinetobacter: summed feature 3 (C16?:?1?7c, C16?:?1?6c; 24.3-27.2?%), C18?:?1?9c (19.9-22.1?%), C16?:?0 (15.2-22.0?%) and C12?:?0 (9.2-14.2?%). On the basis of the current findings, it is concluded that the six strains represent a novel species, for which the name Acinetobacter kookii sp. nov. is proposed. The type strain is 11-0202(T) (?=?KCTC 32033(T)?=?JCM 18512(T)). PMID:23950148

Choi, Ji Young; Ko, Gwangpyo; Jheong, Weonghwa; Huys, Geert; Seifert, Harald; Dijkshoorn, Lenie; Ko, Kwan Soo

2013-12-01

136

Pseudomonas punonensis sp. nov., isolated from straw.  

PubMed

During a study of the 'tunta' (frozen-dry potato) production process in Peru, a bacterial strain, LMT03(T), was isolated from the straw grass in which the potatoes are dried. This strain was classified into the genus Pseudomonas on the basis of the 16S rRNA gene sequence analysis, and is most closely related to Pseudomonas argentinensis CH01(T) with 99.3?% identity in this gene and 96?%, 92?% and 86?% identities in rpoB, rpoD and gyrB genes, respectively. Strain LMT03(T) has a single polar flagellum, like other related yellow-pigment-producing pseudomonads. The major quinone is Q-9. The major fatty acids are C18?:?1?7c in summed feature 8 (40.82?%), C16?:?1?6c/C16?:?1?6c in summed feature 3 (23.72?%) and C16?:?0 (15.20?%). The strain produces oxidase but it does not produce gelatinase, indole, urease, arginine dihydrolase or ?-galactosidase. Catalase production was very weak after 28 and 48 h incubation on nutrient agar medium. Nitrate reduction is negative. It does not hydrolyse aesculin. The DNA G+C content is 57.8 mol%. DNA-DNA hybridization results showed lower than 52?% relatedness with respect to the type strain of P. argentinensis, CH01(T). These results, together with other phenotypic characteristics, support the definition of a novel species within the genus Pseudomonas, for which the name Pseudomonas punonensis sp. nov. is proposed. The type strain is LMT03(T) (?=?LMG 26839(T)?=?CECT 8089(T)). PMID:23002045

Ramos, Elena; Ramírez-Bahena, Martha-Helena; Valverde, Angel; Velázquez, Encarna; Zúñiga, Doris; Velezmoro, Carmen; Peix, Alvaro

2013-05-01

137

Cellulosimicrobium terreum sp. nov., isolated from soil.  

PubMed

A Gram-positive, yellow-pigmented, non-motile and rod-shaped or coccoid bacterial strain, DS-61T, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. The strain grew optimally at pH 6.5-7.5 and 25 degrees C in the presence of 1.0% (w/v) NaCl. Strain DS-61T had peptidoglycan of the type based on L-Lys-L-Thr-D-Asp and contained galactose as the only whole-cell sugar. MK-9(H4) was the predominant menaquinone and anteiso-C15:0 and iso-C15:0 were the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The DNA G+C content was 72.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-61T is most closely affiliated to the genus Cellulosimicrobium, clustering with Cellulosimicrobium cellulans and Cellulosimicrobium funkei. The levels of 16S rRNA gene sequence similarity between strain DS-61T and the type strains of Cellulosimicrobium cellulans and Cellulosimicrobium funkei were 97.4-97.6%. DNA-DNA relatedness data and differential phenotypic properties demonstrated that strain DS-61T is distinguishable from these two recognized Cellulosimicrobium species. On the basis of phenotypic, phylogenetic and genetic data, strain DS-61T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium terreum sp. nov. is proposed. The type strain is DS-61T (=KCTC 19206T=DSM 18665T). An emended description of the genus is given. PMID:17978207

Yoon, Jung-Hoon; Kang, So-Jung; Schumann, Peter; Oh, Tae-Kwang

2007-11-01

138

Isoptericola dokdonensis sp. nov., isolated from soil.  

PubMed

A Gram-positive, non-motile, rod- or coccoid-shaped Isoptericola-like bacterium, strain DS-3(T), was isolated from a soil sample from Dokdo, Korea, and its taxonomic position was investigated by a polyphasic approach. The organism grew optimally at 30 degrees C and pH 7.0-8.0. Strain DS-3(T) had the peptidoglycan type based on l-lys-d-Asp, and galactose, glucose, rhamnose and ribose as the whole-cell sugars. It contained MK-9(H(4)) as the predominant menaquinone and anteiso-C(15 : 0) and iso-C(15 : 0) as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids. The DNA G+C content was 74.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-3(T) was most closely related to members of the genus Isoptericola. Similarity values between the 16S rRNA gene sequences of strain DS-3(T) and the type strains of Isoptericola species ranged from 98.0 to 98.4 %. DNA-DNA relatedness values (11-23 %) and differential phenotypic properties demonstrated that strain DS-3(T) was distinguishable from recognized Isoptericola species. On the basis of phenotypic properties and phylogenetic and genetic distinctiveness, strain DS-3(T) represents a novel species in the genus Isoptericola, for which the name Isoptericola dokdonensis sp. nov. is proposed. The type strain is DS-3(T) (=KCTC 19128(T)=CIP 108921(T)). PMID:17158994

Yoon, Jung-Hoon; Schumann, Peter; Kang, So-Jung; Jung, Seo-Youn; Oh, Tae-Kwang

2006-12-01

139

Streptomyces graminisoli sp. nov. and Streptomyces rhizophilus sp. nov., isolated from bamboo (Sasa borealis) rhizosphere soil.  

PubMed

Four strains of actinomycete, designated strains JR-19T, JR-12, JR-29 and JR-41T were isolated from bamboo (Sasa borealis) rhizosphere soil. Phylogenetic, morphological, chemotaxonomic and phenotypic analysis demonstrated that the four strains belong to the genus Streptomyces. Microscopic observation revealed that the four strains produced spirales spore chains with spiny surfaces. The cell-wall peptidoglycan of the four strains contained ll-diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates mainly contained glucose and ribose. The predominant menaquinones were MK-9 (H6) and MK-9 (H8). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that these strains and the members of the genus Streptomyces exhibited moderately high 16S rRNA gene sequence similarities of 98.3-99.3%, with the most closely related strains being Streptomyces shenzhenensis 172115T and Streptomyces gramineus JR-43T. Based on the phenotypic and genotypic data, the four strains are considered to represent two novel species of the genus Streptomyces, for which the names Streptomyces graminisoli sp. nov. [to accommodate strains JR-19T (type strain; =KACC 16472T=NBRC 108883T), JR-12 (=KACC 16471) and JR-29 (=KACC 16473)] and Streptomyces rhizophilus sp. nov. [for strain JR-41T (=KACC 16580T=NBRC 108885T)] are proposed. PMID:24478213

Lee, Hyo-Jin; Whang, Kyung-Sook

2014-05-01

140

Catabolism of Naphthalenesulfonic Acids by Pseudomonas sp. A3 and Pseudomonas sp. C22  

PubMed Central

Naphthalene and two naphthalenesulfonic acids were degraded by Pseudomonas sp. A3 and Pseudomonas sp. C22 by the same enzymes. Gentisate is a major metabolite. Catabolic activities for naphthalene, 1-naphthalenesulfonic acid, and 2-naphthalenesulfonic acid are induced by growth with naphthalene, 1-naphthalenesulfonic acid, 2-naphthalenesulfonic acid, methylnaphthalene, or salicylate. Gentisate is also an inducer in strain A3. Inhibition kinetics show that naphthalene and substituted naphthalenes are hydroxylated by the same naphthalene dioxygenase. Substrates with nondissociable substituents such as CH3, OCH3, Cl, or NO2 are hydroxylated in the 7,8-position, and 4-substituted salicylates are accumulated. If CO2H, CH2CO2H, or SO3H are substituents, hydroxylation occurs with high regioselectivity in the 1,2-position. Thus, 1,2-dihydroxy-1,2-dihydronaphthalene-2-carboxylic acids are formed quantitatively from the corresponding naphthalenecarboxylic acids. Utilization of naphthalenesulfonic acids proceeds by the same regioselective 1,2-dioxygenation which labilizes the C—SO3? bond and eliminates sulfite. PMID:16345814

Brilon, C.; Beckmann, W.; Knackmuss, H.-J.

1981-01-01

141

[New species, Actinomadura fulvescens sp. nov. and Actinomadura turkmeniaca sp. nov. and their antagonistic properties].  

PubMed

Two new species of Actinomadura isolated from soil samples of the Turkmen SSR, i.e. Actinomadura fulvescens sp. nov. and Actinomadura turkmeniaca sp. nov. are described. The first species is characterized by formation of short (1-2 turns) spiral spore chains, smooth spores, scanty white aerial mycelium, colourless or yellowish substrate mycelium on synthetic media and brownish-yellow substrate mycelium and soluble pigment of the same colour on organic media. No melanoid pigment is secreted. The type culture is designated as INA 3321. The cultures of A. fulvescens show antibiotic activity. A. turkmeniaca is characterized by formation of short straight or spiral spore chains, smooth spores, scanty white aerial mycelium, substrate mycelium and soluble pigment of pinkish-violet colour, absence of melanoid pigment. The type culture is designated as INA 3344. The strains of this species have low antibiotic activity. The study on the use of carbon sources by the representatives of 7 species (9 strains) of Actinomadura showed that the majority of the cultures (5 species, 7 strains) produced no growth on the Pridham and Gottlieb medium (ISP-9) with various carbon sources, including glucose. Possibly this medium cannot be used as the main medium for investigation of the spectrum of carbohydrate consumption in Actinomadura. PMID:6461291

Terekhova, L P; Galatenko, O A; Preobrazhenskaia, T P

1982-02-01

142

Two novel pyrene-degrading Diaphorobacter sp. and Pseudoxanthomonas sp. isolated from soil.  

PubMed

Two new bacterial strains, KOTLB and RN402, which have the ability to utilize pyrene as their sole source of carbon and energy, were isolated from soil. Strains KOTLB and RN402 degraded 99% of 100 mg/l of pyrene in liquid cultures within 16 days. Besides pyrene, both strains also degraded 100 mg/l of phenanthrene almost completely within 8 days and degraded 99% and 55% of 100 mg/l of fluoranthene within 24 days, respectively. 16S rDNA-based phylogenetic analysis suggested that strains KOTLB and RN402 were Diaphorobacter sp. and Pseudoxanthomonas sp., respectively. These two genera have never been reported to be involved in pyrene degradation. Polymerase chain reaction (PCR) amplification with nidA-specific primers revealed the presence of a dioxygenase gene in both strains. The partial nidA gene products of both strains (317 and 384 amino acids, respectively) exhibited 99-100% identity to the NidA proteins, which are the large subunit of terminal dioxygenases for pyrene degradation of Mycobacterium spp. In addition, PCR further demonstrated that nidA genes of these strains were located on a megaplasmid. This is the first report showing that pyrene-degrading Gram-negative bacteria harbor the nidA gene. PMID:19914581

Klankeo, Piriya; Nopcharoenkul, Wannarak; Pinyakong, Onruthai

2009-12-01

143

Aminobacter ciceronei sp. nov. and Aminobacter lissarensis sp. nov., isolated from various terrestrial environments  

USGS Publications Warehouse

The bacterial strains IMB-1T and CC495T, which are capable of growth on methyl chloride (CH3Cl, chloromethane) and methyl bromide (CH3Br, bromomethane), were isolated from agricultural soil in California fumigated with CH3Br, and woodland soil in Northern Ireland, respectively. Two pesticide- /herbicide-degrading bacteria, strains ER2 and C147, were isolated from agricultural soil in Canada. Strain ER2 degrades N-methyl carbamate insecticides, and strain C147 degrades triazine herbicides widely used in agriculture. On the basis of their morphological, physiological and genotypic characteristics, these four strains are considered to represent two novel species of the genus Aminobacter, for which the names Aminobacter ciceronei sp. nov. (type strain IMB-1T=ATCC 202197T=CIP 108660T=CCUG 50580T; strains ER2 and C147) and Aminobacter lissarensis sp. nov. (type strain CC495T=NCIMB 13798T=CIP 108661T=CCUG 50579T) are proposed. ?? 2005 IUMS.

McDonald, I.R.; Kampfer, P.; Topp, E.; Warner, K.L.; Cox, M.J.; Connell, Hancock T.L.; Miller, L.G.; Larkin, M.J.; Ducrocq, V.; Coulter, C.; Harper, D.B.; Murrell, J.C.; Oremland, R.S.

2005-01-01

144

Co-metabolic degradation of tetrabromobisphenol A by novel strains of Pseudomonas sp. and Streptococcus sp.  

PubMed

Three strains capable of rapidly degrading TBBPA by co-metabolism and utilizing formate as the carbon source, named as J-F-01, J-F-02, and J-F-03, respectively, were isolated from enrichment cultures, which have been treated with 0.5mg/L TBBPA for 240d. Based on morphology and 16S rRNA gene sequence analysis, both J-F-01 and J-F-02 were determined to Pseudomonas sp., while J-F-03 was identified as Streptococcus sp. A shorter half-life (6.1d) of TBBPA was observed in pure culture of J-F-03 when compared with J-F-01 (22.5d) and J-F-02 (13.6d). Surprisingly, the degradation of TBBPA was significantly enhanced by the mixed culture of J-F-02 and J-F-03. The optimal degradation conditions for the mixed cultures were determined. Under the optimal conditions, TBBPA (0.5mg/L) was completely metabolized by the mixed culture within ten days. Moreover, bromide and the metabolisms were detected, and a possible metabolic pathway was deduced from the detection of metabolite production patterns. PMID:25062538

Peng, Xingxing; Qu, Xiangdong; Luo, Weishi; Jia, Xiaoshan

2014-10-01

145

Pedobacter huanghensis sp. nov. and Pedobacter glacialis sp. nov., isolated from Arctic glacier foreland.  

PubMed

Two psychrotolerant, Gram-stain-negative, rod-shaped bacterial strains, designed M1-27(T) and 8-24(T), were subjected to polyphasic taxonomic studies. Strain M1-27(T) was isolated from the foreland of the Midtre Lovénbreen glacier, whereas strain 8-24(T) was isolated from the foreland of the Austre Lovénbreen glacier. Both were Arctic glacier forelands, near Ny-Ålesund, Svalbard Archipelago, Norway. Strains M1-27(T) and 8-24(T) exhibited 16S rRNA gene sequence similarities of 91.0-96.0% and 92.3-96.7%, respectively, to type strains of recognized species of the genus Pedobacter. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two strains were grouped with members of the genus Pedobacter, but represented distinct taxa. Both strains contained MK-7 as the predominant menaquinone. The DNA G+C contents of strains M1-27(T) and 8-24(T) were 43.8% and 39.4%, respectively. The phenotypic characteristics, biochemical properties and polygenetic analysis, clearly indicated that strains M1-27(T) (?=?CCTCC AB 2012936(T)?= LMG 28205(T)) and 8-24(T) (?= CCTCC AB 2012941(T)?= NRRL B-59993(T)) represent two novel species of the genus Pedobacter, for which the names Pedobacter huanghensis sp. nov. and Pedobacter glacialis sp. nov., respectively, are proposed. PMID:24763603

Qiu, Xia; Qu, Zhihao; Jiang, Fan; Ren, Lvzhi; Chang, Xulu; Kan, Wenjing; Fang, Chengxiang; Peng, Fang

2014-07-01

146

[Purification and physico-chemical properties of Streptomyces sp. 1349 collagenase and Streptomyces sp. 1382 keratinase].  

PubMed

The schemes of isolation and purification of collagenolytic enzymes of Streptomyces sp. 1349 and keratinolyte enzymes of Streptomyces sp. 1382, which include fractionation by ammonium sulphate separation on TSK-gels: ion-exchange chromatography on Toyopearl DEAE-650(M) and gel-filtration on Toyopearl HW-50, as well as highly efficient liquid chromatography. The purified enzyme preparations proved to be proteases of serine type (collagenase 2 and keratinases) as well as metalloproteases (collagenases 1 and 3). It has seen established that collagenases are enzymes of broad specificity, which are active in respect of proteins of both globular and fibrillar nature. And vice versa, keratinases are proteolytic enzymes of narrow specificity which hydrolyze native keratin. Molecular masses of purified enzyme preparations, from the data of SDS-PAAG are approximately 30-40 kDa (collagenases 1-3) and about 15-20 kDa (keratinases 1 and 2). It is shown that the charged aminoacid residues (about 85%) prevail in enzyme molecules. The enzymes are distinguished by pH- and thermooptima. PMID:15208850

Ivanko, O V; Varbanets', L D

2004-01-01

147

Streptococcus loxodontisalivarius sp. nov. and Streptococcus saliviloxodontae sp. nov., isolated from oral cavities of elephants.  

PubMed

Four Gram-stain-positive, catalase-negative, coccoid-shaped organisms were isolated from elephant oral cavities. The isolates were tentatively identified as streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene sequencing studies confirmed the organisms to be members of the genus Streptococcus. Two isolates (NUM 6304(T) and NUM 6312) were related most closely to Streptococcus salivarius with 96.8?% and 93.1?% similarity based on the 16S rRNA gene and the RNA polymerase ? subunit encoding gene (rpoB), respectively, and to Streptococcus vestibularis with 83.7?% similarity based on the 60 kDa heat-shock protein gene (groEL). The other two isolates (NUM 6306(T) and NUM 6318) were related most closely to S. vestibularis with 97.0?% and 82.9?% similarity based on the 16S rRNA and groEL genes, respectively, and to S. salivarius with 93.5?% similarity based on the rpoB gene. Based on phylogenetic and phenotypic evidence, these isolates are suggested to represent novel species of the genus Streptococcus, for which the names Streptococcus loxodontisalivarius sp. nov. (type strain NUM 6304(T)?=?JCM 19287(T)?=?DSM 27382(T)) and Streptococcus saliviloxodontae sp. nov. (type strain NUM 6306(T)?=?JCM 19288(T)?=?DSM 27513(T)) are proposed. PMID:24994777

Saito, Masanori; Shinozaki-Kuwahara, Noriko; Hirasawa, Masatomo; Takada, Kazuko

2014-09-01

148

ASYMMETRIC SYNTHESIS OF (Rp)- AND (Sp)-2ETHYL, (Rp)-2-PENTYLOXY-, (Sp)-2-PENTYLTHIO- AND (Sp)-2-PENTYLAMINO-4H-1,3,2-BENZODIOXAPHOSPHORIN 2OXIDES  

Microsoft Academic Search

2-Substituted-4H-1,3,2-benzodioxaphosphorin 2-oxides (2-substituted-BDPOs) are potent and stereoselective inhibitors of neuropathy target esterase (NTE) when the 2-substituent is n-alkyl, n-alkyloxy, n-alkylthio or n-alkylamino with maximum potency for the C7 to C9 analogs. Asymmetric syntheses were developed to assign the absolute configurations of each type of 2-substituted-BDPO. (Sp)-O-Methyl ethylphosphonothioic acid, the chiral starting material for (Rp)- and (Sp)-2-ethyl-BDPOs, was obtained by resolution

Shao-Yong Wu; John E. Casida

1994-01-01

149

A new species of Loxosomatidae (Entoprocta, Solitaria) from the White Sea: Loxosomella unicornis sp. nov.  

PubMed

A new solitary entoproct, Loxosomella unicornis sp. nov., is described. The species was found on the gymnolaemate bryozoans Cribrilina sp. and Electra sp. in Kandalaksha Bay, White Sea. Loxosomella unicornis sp. nov. is a medium-sized species with a total length up to 650 µm, eight tentacles and a conspicuous horn-shaped appendage on the top part of calyx.  PMID:25283409

Borisanova, Anastasiya O; Krylova, Elena M

2014-01-01

150

Occurrence of Diplostomum sp. (Trematoda: Diplostomatidae) in Pond-Reared Walleyes from Michigan  

Microsoft Academic Search

Four hundred ninety walleye fingerlings (Stizostedion vitreum) collected in June and November 1986 and in May and June 1987 from seven rearing ponds in Michigan's Lower Peninsula were examined for parasites. Diplostomum sp., Spiroxys sp., and Camallanus sp. were the only parasites found. Of these helminths, Diplostomum sp. occurred most frequently, infecting walleyes from three ponds. Macroscopic pathological conditions associated

Patrick M. Muzzall; Robert D. Sweet; Craig L. Milewski

1990-01-01

151

Description of Sarcocystis anasi sp. nov. and Sarcocystis albifronsi sp. nov. in birds of the order Anseriformes.  

PubMed

On the basis of the already published morphological, 18S rDNA, 28S rDNA data (Kutkien? et al., Parasitol Res 99:562-565, 2006; Parasitol Res 102:691-696, 2008; Parasitol Res 104:329-336, 2009), and ITS-1 region investigation results of sarcocysts presented in this paper, Sarcocystis albifronsi sp. nov. from the white-fronted goose (Anser albifrons) and Sarcocystis anasi sp. nov. from the mallard duck (Anas platyrhynchos) are described. PMID:21845412

Kutkien?, Liuda; Prakas, Petras; Sruoga, Aniolas; Butkauskas, Dalius

2012-02-01

152

Description of Sarcocystis anasi sp. nov. and Sarcocystis albifronsi sp. nov. in birds of the order Anseriformes  

Microsoft Academic Search

On the basis of the already published morphological, 18S rDNA, 28S rDNA data (Kutkien? et al., Parasitol Res 99:562–565, 2006; Parasitol Res 102:691–696, 2008; Parasitol Res 104:329–336, 2009), and ITS-1 region investigation results of sarcocysts presented in this paper, Sarcocystis albifronsi sp. nov. from the white-fronted goose (Anser albifrons) and Sarcocystis anasi sp. nov. from the mallard duck (Anas platyrhynchos)

Liuda Kutkien?; Petras Prakas; Aniolas Sruoga; Dalius Butkauskas

153

Distinct contributions of model MaSp1 and MaSp2 like peptides to the mechanical properties of synthetic major ampullate silk fibers as revealed in silico  

PubMed Central

All characterized major ampullate silks from orb-web weaving spiders are composites of primarily two different proteins: MaSp1 and MaSp2. The conserved association of MaSp1 and MaSp2 in these spider species, the highly conserved amino acid motifs, and variable ratios of MaSp1 to MaSp2 demonstrate the importance of both MaSp1 and MaSp2 to the strength and elasticity of the fiber. Computer simulated mechanical tests predicted differing roles for MaSp1 and MaSp2 in the mechanical properties of the fibers. Recombinant MaSp1 and MaSp2 proteins were blended and spun into fibers mimicking the computer-simulated conditions. Mechanical testing verified the differing roles of MaSp1 and MaSp2. PMID:20657704

Brooks, Amanda E; Nelson, Shane R; Jones, Justin A; Koenig, Courtney; Hinman, Michael; Stricker, Shane; Lewis, Randolph V

2008-01-01

154

Parvicapsula bicornis n. sp. and P. limandae n. sp. (Myxozoa, Parvicapsulidae) in Pleuronectidae (Teleostei, Heterosomata) from Denmark.  

PubMed

Two species of Parvicapsula were found in the kidney tubules and the urinary bladder of 2 pleuronectid fish from the northern Oresund, Denmark. The coelozoic, spherical, disporic trophozoites of both species are 10 to 12 pm in diameter. The myxospores of both species are elongate, asymmetrical and slightly curved, and have spherical polar capsules. Parvicapsula bicornis n. sp. (6-8 x 5-6 microm, polar capsule 2.5 microm in diameter) occurs in Pleuronectes platessa. The polar capsules of P. bicornis are arranged symmetrically on either side of the longitudinal axis and its spores differ from other species of Parvicapsula in having two 2-3 microm long posterior processes of different length. Parvicapsula limandae n. sp. (8-11 x 4-5 pm, polar capsule 1.6 microm in diameter) is found in Limanda limanda. The polar capsules are arranged along the longitudinal axis. It differs from Parvicapsula unicornis Kabata, 1962, recorded from L. limanda, in the arrangement of the polar capsules and in the absence of a posterior horn-like projection. The phylogenetic relationship between P. bicornis n. sp., P. limandae n. sp. and other Parvicapsula spp. was examined with their partial small subunit rDNA (SSU rDNA) sequences. P. limandae n. sp. and P. asymmetrica appear to be closely related, while P. bicornis n. sp. and P. minibicornis are the most divergent members of the genus. PMID:17760385

Køie, Marianne; Karlsbakk, Egil; Nylund, Are

2007-06-29

155

Effects of cell growth and a mobile genetic element on propagation of the phages SP16 and SP-beta in Bacillus subtilis  

E-print Network

Two studies were performed on Bacillus subtilis phages SP16 and SP-beta to characterize the effects of growth and an integrative conjugative element, ICEBs1, respectively. I found that B. subtilis strains are significantly ...

England, Eleina M. (Eleina Helen Maria)

2014-01-01

156

Chitinophaga eiseniae sp. nov., isolated from vermicompost.  

PubMed

A Gram-negative, rod-shaped bacterial strain, YC6729(T), was isolated from vermicompost collected at Masan, Korea, and its taxonomic position was investigated by a polyphasic taxonomic approach. Strain YC6729(T) grew optimally at 30 °C and at pH 6.5-8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC6729(T) belongs to the genus Chitinophaga in the family Chitinophagaceae. It was related most closely to Chitinophaga terrae KP01(T) (96.4?% 16S rRNA gene sequence similarity), Chitinophaga ginsengisegetis Gsoil 040(T) (96.1?%), Chitinophaga arvensicola IAM 12650(T) (96.1?%) and Chitinophaga pinensis DSM 2588(T) (93.3?%). Strain YC6729(T) contained MK-7 as the major menaquinone and homospermidine as the major polyamine. The fatty acids of strain YC6729(T) were iso-C(15?:?0), C(16?:?1)?5c, iso-C(17?:?0) 3-OH, C(16?:?0), anteiso-C(18?:?0) and/or C(18?:?2)?6,9c, iso-C(15?:?0) 2-OH and/or C(16?:?1)?7c, C(14?:?0), iso-C(15?:?0) 3-OH, iso-C(15?:?1) G, C(18?:?1)?5c, iso-C(15?:?1) I and/or C(13?:?0) 3-OH, C(13?:?0) 2-OH, C(16?:?0) 3-OH and unknown fatty acid ECL 13.565. The polar lipid profile contained phosphatidylethanolamine, unknown aminolipids and unknown lipids. The total DNA G+C content of strain YC6729(T) was 48.9 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed that strain YC6729(T) represents a novel species of the genus Chitinophaga, for which the name Chitinophaga eiseniae sp. nov. is proposed. The type strain is YC6729(T) (?=?KACC 13774(T) ?=?DSM 22224(T)). PMID:21057055

Yasir, Muhammad; Chung, Eu Jin; Song, Geun Cheol; Bibi, Fehmida; Jeon, Che Ok; Chung, Young Ryun

2011-10-01

157

In Vitro Antimicrobial Potential of the Lichen Parmotrema sp. Extracts against Various Pathogens  

PubMed Central

Objective(s): The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. The perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. Lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. This study was aimed to explore in vitro antimicrobial activity of lichen Parmotrema sp. Material and Methods: The methanol and aqueous extracts of lichen Parmotrema sp. was extracted using Soxhlet extractor. Antibiotic assessment of methanol and aqueous extracts was done against eight bacterial (Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Shigella sp., Enterococci faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae,) clinical pathogens and five plant pathogenic fungal strains (Aspergillus terreus strain JAS1, Scedosporium sp. JAS1, Ganoderma sp. JAS4, Candida tropicalis and Fusarium sp.) by Kirby-Bauer method. Results: The methanol lichen Parmotrema sp. extract inhibited all the test organisms. The highest antibacterial activity was found against Pseudomonas aeruginosa and Staphylococcus aureus. The weakest activity was manifested in Salmonella sp. and Scedosporium sp. JAS1. Strong antifungal effect was found against Ganoderma sp. JAS4 and Fusarium sp. The aqueous lichen Parmotrema sp. extract revealed neither antibacterial nor antifungal activity. Conclusion: The present study shows that tested lichen Parmotrema sp. extracts demonstrated a strong antimicrobial effect. That suggests the active components from methanol extracts of the investigated lichen Parmotrema sp. can be used as natural antimicrobial agent against pathogens. PMID:23997920

Chauhan, Ritika; Abraham, Jayanthi

2013-01-01

158

Stylobates birtlesi sp. n., a new species of carcinoecium-forming sea anemone... 33 Stylobates birtlesi sp. n., a new species of carcinoecium-  

E-print Network

Stylobates birtlesi sp. n., a new species of carcinoecium-forming sea anemone... 33 Stylobates birtlesi sp. n., a new species of carcinoecium- forming sea anemone (Cnidaria,Actiniaria,Actiniidae) from DG, Wallace CC (2011) Stylobates birtlesi sp. n., a new species of carcinoecium-forming sea anemone

Fautin, Daphne

159

Methylobacterium haplocladii sp. nov. and Methylobacterium brachythecii sp. nov., isolated from bryophytes.  

PubMed

Pink-pigmented, facultatively methylotrophic bacteria, strains 87e(T) and 99b(T), were isolated from the bryophytes Haplocladium microphyllum and Brachythecium plumosum, respectively. The cells of both strains were Gram-reaction-negative, motile, non-spore-forming rods. On the basis of 16S rRNA gene sequence similarity, strains 87e(T) and 99b(T) were found to be related to Methylobacterium organophilum ATCC 27886(T) (97.1% and 97.7%, respectively). Strains 87e(T) and 99b(T) showed highest 16S rRNA gene similarity to Methylobacterium gnaphalii 23e(T) (98.3 and 99.0%, respectively). The phylogenetic similarities to all other species of the genus Methylobacterium with validly published names were less than 97%. Major cellular fatty acids of both strains were C(18:1)?7c and C(18:0). The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrix-assisted, laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains 87e(T) and 99b(T) from their phylogenetically closest relatives. We propose that strains 87e(T) and 99b(T) represent novel species within the genus Methylobacterium, for which the names Methylobacterium haplocladii sp. nov. (type strain 87e(T)?=DSM 24195(T)?=NBRC 107714(T)) and Methylobacterium brachythecii sp. nov. (type strain 99b(T)?=DSM 24105(T)?=NBRC 107710(T)) are proposed. PMID:23475343

Tani, Akio; Sahin, Nurettin

2013-09-01

160

Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov., isolated from the desert.  

PubMed

Two strains of bacteria, JC213(T) and JC215(T), were isolated from desert soil. Colonies were red to pink and cells Gram-stain-negative. Both strains were oxidase- and catalase-positive and hydrolysed casein. In both strains, phosphatidylethanolamine was the major polar lipid, iso-C15?:?0 was the major fatty acid and the bacteriohopane derivative, BHD1, was the major hopanoid. The genomic DNA G+C contents of strains JC213(T) and JC215(T) were 52.7 and 46.3 mol%, respectively. 16S rRNA gene sequence comparisons indicated that both strains belong to the genus Pontibacter within the family Cytophagaceae and the phylum Bacteroidetes. Strain JC213(T) showed the highest sequence similarity to Pontibacter populi HLY7-15(T) (96.6?%) and with other species of the genus Pontibacter sequence similarity was less than 96?%. Strain JC215(T) exhibited highest sequence similarity with Pontibacter lucknowensis DM9(T) (95.1?%) and shared 95?% or less sequence similarity with other species of the genus Pontibacter. The sequence similarity between strains JC213(T) and JC215(T) was 95.8?%. Distinct morphological, physiological and genotypic differences from previously described taxa support JC213(T) and JC215(T) being representatives of two novel species of the genus Pontibacter, for which the names Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov. are proposed and the type strains are JC213(T) (?=?KCTC 32442(T)?=?LMG 27669(T)) and JC215(T) (?=?KCTC 32443(T)?=?LMG 27670(T)), respectively. PMID:24425823

Subhash, Y; Sasikala, Ch; Ramana, Ch V

2014-03-01

161

Nocardioides endophyticus sp. nov. and Nocardioides conyzicola sp. nov., isolated from herbaceous plant roots.  

PubMed

Two Gram-stain-positive, non-motile, non-spore-forming, rod-shaped actinobacterial strains were isolated from the surface-sterilized roots of mugwort (Artemisia princeps) and horse-weed (Conyza canadensis), and subjected to taxonomic characterization. 16S rRNA gene sequence analysis indicated that the isolates, designated MWE 3-5(T) and HWE 2-02(T), should be placed in the genus Nocardioides of the family Nocardioidaceae. The strains were closely related to Nocardioides hankookensis DS-30(T), which exhibited 16S rRNA gene sequence similarity values of 97.99 and 99.09?% with strains MWE 3-5(T) and HWE 2-02(T), respectively. The genome relatedness of N. hankookensis DS-30(T) with strain MWE 3-5(T) was 35.8?%, and that with strain HWE 2-02(T) was 36.4?%, whereas that between the two isolates was 43.2?%. Strains MWE 3-5(T) and HWE 2-02(T) possessed MK-8(H4) as the major isoprenoid quinone, and ll-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids were iso-C16?:?0, iso-C17?:?0 and C18?:?1?9c for strain MWE 3-5(T) and iso-C16?:?0, 10-methyl C18?:?0 and C18?:?1?9c for strain HWE 2-02(T). Based on phenotypic, genotypic and phylogenetic studies, the following two novel species are proposed: Nocardioides endophyticus sp. nov. (type strain, MWE 3-5(T)?=?KCTC 29122(T)?=?JCM 18532(T)) and Nocardioides conyzicola sp. nov. (type strain, HWE 2-02(T)?=?KCTC 29121(T)?=?JCM 18531(T)). PMID:23990649

Han, Ji-Hye; Kim, Tae-Su; Joung, Yochan; Kim, Mi Na; Shin, Kee-Sun; Bae, Taeok; Kim, Seung Bum

2013-12-01

162

Embarazo abdominal secundario tras una esterilización tubárica  

Microsoft Academic Search

The ectopic pregnancy appears in the 10-12% of cases after tubal sterilization and it is usually located in the tube. 1% of ectopic pregnancies are abdominal and the implantation in omentum is a rare condition with only 16 described cases. According to Studdiford, they can be classified as primary or secondary depending on whether they are originated or not in

Elena M. Losa Pajares; Marco A. Arones Collantes; Leyre Gil Martínez-Acacio; Alejandro Pascual Martín; Vicente Almodóvar Rico; Gaspar González de Merlo

2007-01-01

163

Oil productivity of the tropical marine diatom Thalassiosira sp.  

PubMed

To understand the potential of cultivating tropical marine diatom Thalassiosira sp. to produce biofuel, biodiesel product properties and growth characteristics of Thalassiosira sp. in three different media were investigated. After medium evaluation, significant Thalassiosira sp. cell growth was observed in both Walne and enriched seawater media, but not in plain seawater medium. The microalgae grew well in alkaline condition (pH range of 8.0-8.8). The average biomass density cultured in Walne and enriched seawater media on the 6th day was 4.36 and 2.50 g L(-1), respectively. Based on ESI-IT-MS spectra, the TAGs of algal oil were identified as POP, POO, and SOO, and the FAMEs as oleic acid methyl ester. The oil productivity of Thalassiosira sp. cultured in Walne and enriched seawater media were 150 and 290 ?L L(-1) d(-1), respectively. The density and kinematic viscosity of Thalassiosira sp. biodiesel were 0.857 g mL(-1) and 1.151 mm(2) s(-1). PMID:22264432

Nurachman, Zeily; Hartati; Anita, Syahfitri; Anward, Etsuroyya Ewidyasari; Novirani, Gestria; Mangindaan, Bill; Gandasasmita, Suryo; Syah, Yana Maolana; Panggabean, Lily Maria Goretty; Suantika, Gede

2012-03-01

164

Sp(8) invariant higher spin theory, twistors and geometric BRST formulation of unfolded field equations  

NASA Astrophysics Data System (ADS)

We discuss twistor-like interpretation of the Sp(8) invariant formulation of 4d massless fields in ten dimensional Lagrangian Grassmannian Sp(8)/P which is the generalized space-time in this framework. The correspondence space C is SpH(8)/PH where SpH(8) is the semidirect product of Sp(8) with Heisenberg group SpHM and PH is some quasiparabolic subgroup of SpH(8). Spaces of functions on Sp(8)/P and SpH(8)/PH consist of QP closed functions on Sp(8) and QPH closed functions on SpH(8), where QP and QPH are canonical BRST operators of P and PH. The space of functions on the generalized twistor space T identifies with the SpH(8) Fock module. Although T cannot be realized as a homogeneous space, we find a nonstandard SpH(8) invariant BRST operator Q (Q2 = 0) that gives rise to an appropriate class of functions via the condition Qf = 0 equivalent to the unfolded higher-spin equations. The proposed construction is manifestly Sp(8) invariant, globally defined and coordinate independent. Its Minkowski analogue gives a version of twistor theory with both types of chiral spinors treated on equal footing. The extensions to the higher rank case with several Heisenberg groups and to the complex case are considered. A relation with Riemann theta functions, that are Q-closed, is discussed.

Gelfond, O. A.; Vasiliev, M. A.

2009-12-01

165

Aureobasidium thailandense sp. nov. isolated from leaves and wooden surfaces.  

PubMed

Aureobasidium thailandense sp. nov. is described from cultures of material collected on leaves and wooden surfaces in Thailand and the type isolate is NRRL 58539(T). Phylogenetically it is distinct from other species of the genus Aureobasidium. Phenotypically it is distinguished by its cardinal growth temperatures, salt tolerance and production of reddish brown hyphal pigmentation in PDA cultures, but micro-morphologically it is not clearly distinguishable from Aureobasidium pullulans. Unlike A. pullulans, A. thailandense sp. nov. produces a non-pullulan extracellular polysaccharide whose characteristics are unknown. The two known isolates of A. thailandense sp. nov. possess an approx. 500 bp type I intron in the 18S rRNA gene that is present in ITS amplifications using primers ITS4 and ITS5. A. pullulans isolates uniformly lack this intron. PMID:23178722

Peterson, Stephen W; Manitchotpisit, Pennapa; Leathers, Timothy D

2013-02-01

166

Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09.  

PubMed

Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity. PMID:25367790

Chen, Yun-Mei; Xu, Hai-Yang; Wang, Yang; Zhang, Jian-Fa; Wang, Shi-Ming

2014-11-01

167

Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09*  

PubMed Central

Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity. PMID:25367790

Chen, Yun-mei; Xu, Hai-yang; Wang, Yang; Zhang, Jian-fa; Wang, Shi-ming

2014-01-01

168

Microbial Succession during Thermophilic Digestion: The Potential of Methanosarcina sp  

PubMed Central

A distinct succession from a hydrolytic to a hydrogeno- and acetotrophic community was well documented by DGGE (denaturing gradient gel electrophoresis) and dHPLC (denaturing high performance liquid chromatography), and confirmed by qPCR (quantitative PCR) measurements and DNA sequence analyses. We could prove that Methanosarcina thermophila has been the most important key player during the investigated anaerobic digestion process. This organism was able to terminate a stagnation phase, most probable caused by a decreased pH and accumulated acetic acid following an initial hydrolytic stage. The lack in Methanosarcina sp. could not be compensated by high numbers of Methanothermobacter sp. or Methanoculleus sp., which were predominant during the initial or during the stagnation phase of the fermentation, respectively. PMID:24586260

Illmer, Paul; Reitschuler, Christoph; Wagner, Andreas Otto; Schwarzenauer, Thomas; Lins, Philipp

2014-01-01

169

Comparison of green algae Cladophora sp. and Enteromorpha sp. as potential biomonitors of chemical elements in the southern Baltic.  

PubMed

The contents of Cd, Cu, Ni, Pb, Zn, Mn, K, Na, Ca and Mg were determined in the green algae Cladophora sp. from coastal and lagoonal waters of the southern Baltic. Factor analysis demonstrated spatial differences between concentration of chemical elements. The algae from the southern Baltic contained more Na and K while the anthropogenic impact of Cu, Pb and Zn was observed in the case of Cladophora sp. and Enteromorpha sp. from the Gulf of Gdansk at the vicinity of Gdynia. This area is exposed to emission of heavy metals from municipal and industrial sources with the main contribution of shipbuilding industry and seaport. The statistical evaluation of data has demonstrated that there exists a correlation between concentrations of Cu, Pb and Zn in both green algae collected at the same time and sampling sites of the Gulf of Gdansk. Our results show that in the case of absence of one species in the investigated area it is still possible to continue successfully the biomonitoring studies with its replacing by second one, i.e. Cladophora sp. by Enteromorpha sp. and vice versa; in consequence reliable results may be obtained. PMID:17719620

Zbikowski, Rados?aw; Szefer, Piotr; Lata?a, Adam

2007-11-15

170

Polymorphic phases of sp3-hybridized carbon under cold compression.  

PubMed

It is well established that graphite can be transformed into superhard carbons under cold compression (Mao et al. Science 2003, 302, 425). However, structure of the superhard carbon is yet to be determined experimentally. We have performed an extensive structural search for the high-pressure crystalline phases of carbon using the evolutionary algorithm. Nine low-energy polymorphic structures of sp(3)-hybridized carbon result from the unbiased search. These new polymorphic carbon structures together with previously reported low-energy sp(3)-hybridized carbon structures (e.g., M-carbon, W-carbon, and Cco-C(8) or Z-carbon) can be classified into three groups on the basis of different ways of stacking two (or more) out of five (A-E) types of buckled graphene layers. Such a classification scheme points out a simple way to construct a variety of sp(3)-hybridized carbon allotropes via stacking buckled graphene layers in different combinations of the A-E types by design. Density-functional theory calculations indicate that, among the nine low-energy crystalline structures, seven are energetically more favorable than the previously reported most stable crystalline structure (i.e., Cco-C(8) or Z-carbon) in the pressure range 0-25 GPa. Moreover, several newly predicted polymorphic sp(3)-hybridized carbon structures possess elastic moduli and hardness close to those of the cubic diamond. In particular, Z-carbon-4 possesses the highest hardness (93.4) among all the low-energy sp(3)-hybridized carbon structures predicted today. The calculated electronic structures suggest that most polymorphic carbon structures are optically transparent. The simulated X-ray diffraction (XRD) spectra of a few polymorphic structures are in good agreement with the experimental spectrum, suggesting that samples from the cold-compressed graphite experiments may consist of multiple polymorphic phases of sp(3)-hybridized carbon. PMID:22490007

Zhou, Rulong; Zeng, Xiao Cheng

2012-05-01

171

Fuels irradiation testing for the SP-100 program  

NASA Technical Reports Server (NTRS)

An SP-100 fuel pin irradiation testing program is well on the way to providing data for performance correlations and demonstrating the lifetime and safety of the fuel system of the compact lithium-cooled reactor. Key SP-100 fuel performance issues addressed are the need for low fuel swelling and low fission gas release to minimize cladding strain, and the need for barrier integrity to prevent fuel/cladding chemical interaction. This paper provides a description of the irradiation test program that addresses these key issues and summarizes recent results of posttest examinations including data obtained at 6 atom percent goal burnup.

Makenas, Bruce J.; Hales, Janell W.; Ward, Alva L.

1991-01-01

172

Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., Isolated from Sea Water.  

PubMed

Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1T and A3d10T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4°C and 40°C, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C16:0, C16:1?7c, C18:1?9c and C18:1?7c. The G+C content of the DNA for strains R9SW1T and A3d10T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved protein-coding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1T clusters with M. algicola DG893T sharing 99.40%, and A3d10T clusters with M. sediminum R65T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1T and A3d10T represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1T (?=? LMG 27497T ?=? JCM 19399T ?=? CIP 110588T ?=? KMM 7502T) and Marinobacter similis sp. nov., with the type strain A3d10T (?=? JCM 19398T ?=? CIP 110589T ?=? KMM 7501T), are proposed. PMID:25198502

Ng, Hooi Jun; López-Pérez, Mario; Webb, Hayden K; Gomez, Daniela; Sawabe, Tomoo; Ryan, Jason; Vyssotski, Mikhail; Bizet, Chantal; Malherbe, François; Mikhailov, Valery V; Crawford, Russell J; Ivanova, Elena P

2014-01-01

173

Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., Isolated from Sea Water  

PubMed Central

Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1T and A3d10T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4°C and 40°C, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C16:0, C16:1?7c, C18:1?9c and C18:1?7c. The G+C content of the DNA for strains R9SW1T and A3d10T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved protein-coding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1T clusters with M. algicola DG893T sharing 99.40%, and A3d10T clusters with M. sediminum R65T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1T and A3d10T represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1T (?=? LMG 27497T ?=? JCM 19399T ?=? CIP 110588T ?=? KMM 7502T) and Marinobacter similis sp. nov., with the type strain A3d10T (?=? JCM 19398T ?=? CIP 110589T ?=? KMM 7501T), are proposed. PMID:25198502

Ng, Hooi Jun; López-Pérez, Mario; Webb, Hayden K.; Gomez, Daniela; Sawabe, Tomoo; Ryan, Jason; Vyssotski, Mikhail; Bizet, Chantal; Malherbe, François; Mikhailov, Valery V.; Crawford, Russell J.; Ivanova, Elena P.

2014-01-01

174

Microbacterium kyungheense sp. nov. and Microbacterium jejuense sp. nov., isolated from salty soil.  

PubMed

Two novel strains, THG-C26(T) and THG-C31(T), were characterized using a polyphasic approach to determine their taxonomic positions. These two isolates were aerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped. 16S rRNA gene sequences and phenotypic features including chemotaxonomic characteristics indicated that the two isolates clearly represented members of the genus Microbacterium. The quinone systems of strains THG-C26(T) and THG-C31(T) contained MK-12/MK-13 as major menaquinones. The diamino acid in cell-wall hydrolysates of the two strains was ornithine. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polyamine pattern had spermidine as the predominant component. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipids. Phenotypic characteristics supported the affiliation of strains THG-C26(T) and THG-C31(T) to the genus Microbacterium. Chemotaxonomic data and DNA-DNA relatedness values allowed differentiation of these strains from other species of the genus Microbacterium with validly published names. Strains THG-C26(T) and THG-C31(T) showed highest 16S rRNA gene sequence similarities with Microbacterium resistens DMMZ 1710(T) (98.5%) and Microbacterium trichothecenolyticum IFO 15077(T) (98.8%), respectively, and the 16S rRNA gene sequence similarity between them was 99.0%. DNA-DNA hybridization values between the novel isolates and strains of other species of the genus Microbacterium with validly published names were 4-25%. Therefore, strains THG-C26(T) and THG-C31(T) are considered to represent two novel species of the genus Microbacterium, for which the names Microbacterium kyungheense sp. nov. [type strain THG-C26(T) (?= KACC 17124(T)?= JCM 18735(T))] and Microbacterium jejuense sp. nov. [type strain THG-C31(T) (?= KACC 17123(T)?= JCM 18734(T))] are proposed. PMID:24729394

Kook, MooChang; Son, Heung-Min; Yi, Tae-Hoo

2014-07-01

175

Bacillus beijingensis sp. nov. and Bacillus ginsengi sp. nov., isolated from ginseng root.  

PubMed

Four alkaligenous, moderately halotolerant strains, designated ge09, ge10(T), ge14(T) and ge15, were isolated from the internal tissue of ginseng root and their taxonomic positions were investigated by using a polyphasic approach. Cells of the four strains were Gram-positive-staining, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains ge09 and ge10(T) formed one cluster and strains ge14(T) and ge15 formed another separate cluster within the genus Bacillus. 16S rRNA gene sequence similarities with type strains of other Bacillus species were less than 97 %. Levels of DNA-DNA relatedness among the four strains showed that strains ge09 and ge10(T) and strains ge14(T) and ge15 belonged to two separate species; the mean level of DNA-DNA relatedness between ge10(T) and ge14(T) was only 28.7 %. Their phenotypic and physiological properties supported the view that the two strains represent two different novel species of the genus Bacillus. The DNA G+C contents of strains ge10(T) and ge14(T) were 49.9 and 49.6 mol%, respectively. Strains ge10(T) and ge14(T) showed the peptidoglycan type A4alpha l-Lys-d-Glu. The lipids present in strains ge10(T) and ge14(T) were diphosphatidylglycerol, phosphatidylglycerol, a minor amount of phosphatidylcholine and two unknown phospholipids. Their predominant respiratory quinone was MK-7. The fatty acid profiles of the four novel strains contained large quantities of branched and saturated fatty acids. The predominant cellular fatty acids were iso-C(15 : 0) (42.5 %), anteiso-C(15 : 0) (22.2 %), anteiso-C(17 : 0) (7.3 %) and C(16 : 1)omega7c alcohol (5.7 %) in ge10(T) and iso-C(15 : 0) (50.7 %) and anteiso-C(15 : 0) (20.1 %) in ge14(T). On the basis of their phenotypic properties and phylogenetic distinctiveness, two novel species of the genus Bacillus are proposed, Bacillus beijingensis sp. nov. (type strain ge10(T) =DSM 19037(T) =CGMCC 1.6762(T)) and Bacillus ginsengi sp. nov. (type strain ge14(T) =DSM 19038(T) =CGMCC 1.6763(T)). PMID:19329597

Qiu, Fubin; Zhang, Xiaoxia; Liu, Lin; Sun, Lei; Schumann, Peter; Song, Wei

2009-04-01

176

Natural infection by Giardia sp. and Cryptosporidium sp. in dairy goats, associated with possible risk factors of the studied properties.  

PubMed

Visits were made to six farms raising dairy goats located in the mountain region of the state of Rio de Janeiro, Brazil, seeking to identify parasitism by Giardia sp. and Cryptosporidium sp. During the visits, fecal samples were collected from approximately 10% of the stock of each property. A questionnaire was given to the keepers on each property to obtain data for epidemiological analysis. A total of 105 fecal samples was collected, 56 from adult animals (over 12 months of age) and 49 from juvenile animals (less than 12 months). The fecal material was processed and subjected to the centrifuge-flotation technique and to staining with safranine-methylene blue. Protozoans of the genus Cryptosporidium were found at two properties, where the hygiene conditions of the installations were considered average and the stalls were made of wood slats raised from the ground. A total of five (4.8%) of the samples was found to be positive for the presence of this protozoan, all from juvenile animals. Cysts of the genus Giardia were found at two properties. Of the 105 samples analyzed, the protozoan was detected in 15 (14.3%), all in juvenile animals, and animals in the age range of 1-3 months had significantly more infections. Age, sanitary condition of the stalls and stalls made of wood slats and raised from the ground, can be pointed out as possible risk factors for infection by Giardia sp. and Cryptosporidium sp. The study reports for the first time the occurrence of Cryptosporidium sp. and Giardia sp. in goats in the state of Rio de Janeiro. PMID:16105719

Bomfim, T C B; Huber, F; Gomes, R S; Alves, L L

2005-11-25

177

Fusarium euwallaceae sp. nov.--a symbiotic fungus of Euwallacea sp., an invasive ambrosia beetle in Israel and California.  

PubMed

The invasive Asian ambrosia beetle Euwallacea sp. (Coleoptera, Scolytinae, Xyleborini) and a novel Fusarium sp. that it farms in its galleries as a source of nutrition causes serious damage to more than 20 species of live trees and pose a serious threat to avocado production (Persea americana) in Israel and California. Adult female beetles are equipped with mandibular mycangia in which its fungal symbiont is transported within and from the natal galleries. Damage caused to the xylem is associated with disease symptoms that include sugar or gum exudates, dieback, wilt and ultimately host tree mortality. In 2012 the beetle was recorded on more than 200 and 20 different urban landscape species in southern California and Israel respectively. Euwallacea sp. and its symbiont are closely related to the tea shot-hole borer (E. fornicatus) and its obligate symbiont, F. ambrosium occurring in Sri Lanka and India. To distinguish these beetles, hereafter the unnamed xyleborine in Israel and California will be referred to as Euwallacea sp. IS/CA. Both fusaria exhibit distinctive ecologies and produce clavate macroconidia, which we think might represent an adaption to the species-specific beetle partner. Both fusaria comprise a genealogically exclusive lineage within Clade 3 of the Fusarium solani species complex (FSSC) that can be differentiated with arbitrarily primed PCR. Currently these fusaria can be distinguished only phenotypically by the abundant production of blue to brownish macroconidia in the symbiont of Euwallacea sp. IS/CA and their rarity or absence in F. ambrosium. We speculate that obligate symbiosis of Euwallacea and Fusarium, might have driven ecological speciation in these mutualists. Thus, the purpose of this paper is to describe and illustrate the novel, economically destructive avocado pathogen as Fusarium euwallaceae sp. nov. S. Freeman et al. PMID:23928415

Freeman, S; Sharon, M; Maymon, M; Mendel, Z; Protasov, A; Aoki, T; Eskalen, A; O'Donnell, K

2013-01-01

178

Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.  

PubMed

Thirty-four Acetobacter strains, representing Acetobacter aceti, Acetobacter pasteurianus, Acetobacter pomorum, Acetobacter peroxydans, Acetobacter lovaniensis, Acetobacter estunensis, Acetobacter orleanensis, Acetobacter indonesiensis and Acetobacter tropicalis, were subjected to a polyphasic study that included DNA-DNA hybridizations, DNA base ratio determinations, 16S rDNA sequence analysis and phenotypic characterization. Two novel species are proposed, Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. The type strains of these species are respectively LMG 1625T (= DSM 14362T = NCIB 8894T = ATCC 23765T) and LMG 1746T (= DSM 14337T). PMID:12361257

Cleenwerck, I; Vandemeulebroecke, K; Janssens, D; Swings, J

2002-09-01

179

Structural studies of the polysaccharides from the lipopolysaccharides of Azospirillum brasilense Sp246 and SpBr14.  

PubMed

Lipopolysaccharides from closely related Azospirillum brasilense strains, Sp246 and SpBr14, were obtained by phenol-water extraction. Mild acid hydrolysis of the lipopolysaccharides followed by GPC on Sephadex G-50 resulted in polysaccharide mixtures. On the basis of sugar and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy data, it was concluded that both bacteria possess the same two distinct polysaccharides having structures 1 and 2: Structure 1 has been reported earlier for a polysaccharide of A. brasilense 54 [Fedonenko et al., 2011](6) whereas to our knowledge structure 2 has not been hitherto found in bacterial polysaccharides. PMID:25240180

Sigida, Elena N; Fedonenko, Yuliya P; Shashkov, Alexander S; Grinev, Vyacheslav S; Zdorovenko, Evelina L; Konnova, Svetlana A; Ignatov, Vladimir V; Knirel, Yuriy A

2014-10-29

180

Rosenbergiella australoborealis sp. nov., Rosenbergiella collisarenosi sp. nov. and Rosenbergiella epipactidis sp. nov., three novel bacterial species isolated from floral nectar.  

PubMed

The taxonomic status of nine strains of the family Enterobacteriaceae isolated from floral nectar of wild Belgian, French, South African and Spanish insect-pollinated plants was investigated following a polyphasic approach. Confirmation that these strains belonged to the genus Rosenbergiella was obtained from comparative analysis of partial sequences of the 16S rRNA gene and other core housekeeping genes (atpD [ATP synthase ?-chain], gyrB [DNA gyrase subunit B] and rpoB [RNA polymerase ?-subunit]), DNA-DNA reassociation data, determination of the DNA G+C content and phenotypic profiling. Two strains belonged to the recently described species Rosenbergiella nectarea, while the other seven strains represented three novel species within the genus Rosenbergiella. The names Rosenbergiella australoborealis sp. nov. (with strain CdVSA 20.1(T) [LMG 27954(T)=CECT 8500(T)] as the type strain), Rosenbergiella collisarenosi sp. nov. (with strain 8.8A(T) [LMG 27955(T)=CECT 8501(T)] as the type strain) and Rosenbergiella epipactidis sp. nov. (with strain 2.1A(T) [LMG 27956(T)=CECT 8502(T)] as the type strain) are proposed. Additionally, the description of the genus Rosenbergiella is updated on the basis of new phenotypic and molecular data. PMID:24794950

Lenaerts, Marijke; Alvarez-Pérez, Sergio; de Vega, Clara; Van Assche, Ado; Johnson, Steven D; Willems, Kris A; Herrera, Carlos M; Jacquemyn, Hans; Lievens, Bart

2014-09-01

181

Phytohabitans flavus sp. nov., Phytohabitans rumicis sp. nov. and Phytohabitans houttuyneae sp. nov., isolated from plant roots, and emended description of the genus Phytohabitans.  

PubMed

An actinomycete strain, designated K09-0627(T), was isolated from the roots of an orchid collected in Okinawa Prefecture, Japan. Two actinomycete strains K11-0047(T) and K11-0057(T) were isolated from the roots of Rumex acetosa and Houttuynia cordata collected in Kanagawa Prefecture, Japan. 16S rRNA gene sequence analyses indicated that the isolates belonged to the genus Phytohabitans, and that they were closely related to each other and to Phytohabitans suffuscus K07-0523(T). The DNA-DNA relatedness values between the three isolates and Phytohabitans suffuscus were below 70%. On the basis of phylogenetic analysis, DNA-DNA relatedness values and phenotypic characteristics, the strains should be classified as novel species in the genus Phytohabitans, for which the names Phytohabitans flavus sp. nov. (type strain, K09-0627(T)=JCM 17387(T)=NBRC 107702(T)=DSM 45551(T)), Phytohabitans rumicis sp. nov. (type strain, K11-0047(T)=JCM 17829(T)=NBRC 108638(T)=BCC 48146(T)) and Phytohabitans houttuyneae sp. nov. (type strain, K11-0057(T)=JCM 17830(T)=NBRC 108639(T)=BCC 48147(T)) are proposed. PMID:22228668

Inahashi, Yuki; Matsumoto, Atsuko; Omura, Satoshi; Takahashi, Yoko

2012-11-01

182

Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes.  

PubMed

Gram-negative, rod-shaped bacteria were isolated from root nodules of Lupinus polyphyllus, Lathyrus latifolius and Robinia pseudoacacia. Based on the 16S rRNA gene phylogeny, they were closely related to Bosea species (100-97?% similarity), belonging to the class Alphaproteobacteria, family Bradyrhizobiaceae. The closest relatives of LMG 26383(T), LMG 26379(T) and LMG 26381(T) were respectively the type strains of Bosea thiooxidans (99.6?%), B. eneae (98.3?%) and B. minatitlanensis (99.0?%). Chemotaxonomic data, including major fatty acid profiles, supported the assignment of our strains to the genus Bosea. Analysis of the concatenated sequences of five housekeeping genes (atpD, dnaK, gyrB, recA and rpoB) and the results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from each other and from the five Bosea species with validly published names. No nodA or nodC genes could be amplified, while nifH PCR gave non-specific products. On the basis of genotypic and phenotypic data, three novel species, Bosea lupini sp. nov. (type strain LMG 26383(T) ?=?CCUG 61248(T) ?=?R-45681(T)), Bosea lathyri sp. nov. (type strain LMG 26379(T) ?=?CCUG 61247(T) ?=?R-46060(T)) and Bosea robiniae sp. nov. (type strain LMG 26381(T) ?=?CCUG 61249(T) ?=?R-46070(T)), are proposed. PMID:22155761

De Meyer, Sofie E; Willems, Anne

2012-10-01

183

Metschnikowia chrysoperlae sp. nov., Candida picachoensis sp. nov. and Candida pimensis sp. nov., isolated from the green lacewings Chrysoperla comanche and Chrysoperla carnea (Neuroptera: Chrysopidae).  

PubMed

Fourteen yeast isolates comprising three taxa were cultured from digestive tracts of adult Chrysoperla species (Neuroptera: Chrysopidae) and their eggs. The yeast taxa were distinguished based on an estimated molecular phylogeny, DNA sequences and traditional taxonomic criteria. The new yeasts are closely related to Metschnikowia pulcherrima but are sufficiently distinguished by sequence comparison of rRNA gene sequences to consider them as novel species. Here, three novel species are described and their relationships with other taxa in the Saccharomycetes are discussed. Metschnikowia chrysoperlae sp. nov. (type strain, NRRL Y-27615T = CBS 9803T) produced needle-shaped ascospores and was the only teleomorph found. Large numbers of chlamydospores similar to those observed in M. pulcherrima were also produced. The other two novel species are asexual yeasts, Candida picachoensis sp. nov. (type strain, NRRL Y-27607T = CBS 9804T) and Candida pimensis sp. nov. (type strain, NRRL Y-27619T = CBS 9805T), sister taxa of M. chrysoperlae and M. pulcherrima. A specialized relationship between yeasts and lacewing hosts may exist, because the yeasts were isolated consistently from lacewings only. Although M. chrysoperlae was isolated from eggs and adult lacewings, suggesting the possibility of vertical transmission, no yeast was isolated from larvae. PMID:15388758

Suh, Sung-Oui; Gibson, Cara M; Blackwell, Meredith

2004-09-01

184

Possible linkage of SP6 transcriptional activity with amelogenesis by protein stabilization.  

PubMed

Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development. The molecular mechanisms of ameloblast differentiation (amelogenesis) are currently not well understood. SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner. Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis. However, it remains unclear how Sp6 controls amelogenesis. In this study, we used SP6 high producer cells to identify SP6 target genes. Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway. We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis. Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis. PMID:22046099

Utami, Trianna W; Miyoshi, Keiko; Hagita, Hiroko; Yanuaryska, Ryna Dwi; Horiguchi, Taigo; Noma, Takafumi

2011-01-01

185

Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization  

PubMed Central

Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development. The molecular mechanisms of ameloblast differentiation (amelogenesis) are currently not well understood. SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner. Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis. However, it remains unclear how Sp6 controls amelogenesis. In this study, we used SP6 high producer cells to identify SP6 target genes. Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway. We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis. Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis. PMID:22046099

Utami, Trianna W.; Miyoshi, Keiko; Hagita, Hiroko; Yanuaryska, Ryna Dwi; Horiguchi, Taigo; Noma, Takafumi

2011-01-01

186

Antimetastasis effect of anthraquinones from marine fungus, Microsporum sp.  

PubMed

This chapter discusses about obtaining natural products which have anticancer metastasis activities from selected marine-derived fungus (Microsporum sp.) and investigates their biological activities such as cytotoxicity on viability cell lines, anticancer cell migration and invasion, protease inhibition, and expression of matrix metalloproteinase (MMP-2 and -9). Moreover, the correlative mechanisms behind these activities were studied. PMID:22361203

Zhang, Chen; Kim, Se-Kwon

2012-01-01

187

Analysis of Fruit Aroma of Different Crabapple (Malus sp.) Cultivars  

Microsoft Academic Search

Objective) The aim of this experiment was to analyze the fruit aroma of different crabapple (Malus sp.) cultivars and the changes of main volatiles of ripe fruits, and summarize a effective method to search new idioplasms with particular fruit aroma in Malus.(Method)Volatiles of ripe crabapple fruits of six varieties ('Red splender', 'Strawberry parifit', 'Pink Spire', 'Radiant', 'Sparkler', 'Flame') were analyzed

188

INUNDAÇÕES EM SÃO CARLOS, SP: AVALIAÇÃO DE CHUVAS COM RADAR  

Microsoft Academic Search

This work presents three flood events in the urban catchment of the Gregorio creek basin in São Carlos, SP, and the rain evaluation of rain estimated by the Bauru weather radar. The radar rain quantification was made using a Z-R relationship derived from radar reflectivity and total rain from raingage, in these flood cases. Another radar rain quantification for those

Mauricio de Agostinho Antonio

189

Oil from the tropical marine benthic-diatom Navicula sp.  

PubMed

The potential of the tropical marine benthic-diatom Navicula sp. for biodiesel feedstock was investigated. Growth profiles were analyzed by changing nutrient compositions in three different media (Walne, plain seawater, and modified seawater) and irradiance intensities. Navicula sp. cells showed significant growth in Walne and modified seawater medium but not in plain seawater medium. The microalgae grew well in a pH range of 7.8-8.4, and the cells were very sensitive to the intensity of direct sunlight exposure. The average cell concentration obtained from the cultures in plain seawater, Walne, and modified seawater media at the beginning of the stationary phase was 0.70, 2.17, and 2.54 g/L, respectively. Electron spray ionization-ion trap-mass spectrometry showed that the triacylglycerols of the algae oil were identified as POP (palmitic-oleic-palmitic), POO (palmitic-oleic-oleic), and OOLn (oleic-oleic-linoleic). The oil productivity of Navicula sp. cultivated in Walne and modified seawater media was 90 and 124 ?L L(-1) culture d(-1). The Navicula sp. biodiesel exhibited a kinematic viscosity of 1.299 mm(2)/s, density of 0.8347 g/mL, and internal energy of 0.90 kJ/mL. PMID:22926527

Nurachman, Zeily; Brataningtyas, Dewi Susan; Hartati; Panggabean, Lily Maria Goretty

2012-11-01

190

Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium  

PubMed Central

Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various chain lengths and degrees of saturation at the C-3 position. We have isolated a bacterium, RB-44, from a site which was formally a landfill dumping ground. Using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis, this isolate was identified as a Pandoraea sp.which was then screened for AHL production using biosensors which indicated its quorum sensing properties. To identify the AHL profile of Pandoraea sp. RB-44, we used high resolution tandem mass spectrometry confirming that this isolate produced N-octanoylhomoserine lactone (C8-HSL). To the best of our knowledge, this is the first report that showed quorum sensing activity exhibited by Pandoraea sp. Our data add Pandoraea sp. to the growing number of bacteria that possess QS systems. PMID:24145919

Han-Jen, Robson Ee; Wai-Fong, Yin; Kok-Gan, Chan

2013-01-01

191

Complete Genome Assembly of Corynebacterium sp. Strain ATCC 6931  

PubMed Central

The genus Corynebacterium is best known for the pathogen C. diphtheriae; however, it contains mostly commensal and nonpathogenic, as well as several opportunistic, pathogens. Here, we present the 2.47-Mb scaffolded assembly of the type strain, Corynebacterium sp. ATCC 6931 (NCTC 1914), as deposited into GenBank under accession number CP008913. PMID:25342684

Daligault, H. E.; Davenport, K. W.; Minogue, T. D.; Bishop-Lilly, K. A.; Bruce, D. C.; Chain, P. S.; Coyne, S. R.; Frey, K. G.; Jaissle, J.; Koroleva, G. I.; Ladner, J. T.; Li, P-E.; Meincke, L.; Munk, A. C.; Palacios, G. F.; Redden, C. L.

2014-01-01

192

Aspochalasin I, a Melanogenesis Inhibitor from Aspergillus sp  

Microsoft Academic Search

In the course of screening for the melanogenesis inhibitors, aspochalasin I was isolated from solid-state culture of Aspergillus sp. Fb020460. Its structure was determined by spectroscopic analysis including mass spectroscopy and NMR analysis. Aspochalasin I potently inhibited melanogenesis in Mel-Ab cells with an IC50 value of 22.4 µM without cytotoxicity.

Soo-Jin Choo; Bong-Sik Yun; In-Ja Ryoo; Young-Hee Kim; Ki-Hwan Bae; Ick-Dong Yoo

2009-01-01

193

CriSP a Tool for Bump Hunting Jaroslaw Harezlak  

E-print Network

CriSP ­ a Tool for Bump Hunting Jaroslaw Harezlak Indiana University School of Medicine (1971). In the 1980's nonparametric methods in bump­hunting started gaining popularity due to greater) and Heckman (1992) discussed the issues involved with bump hunting in regression but without giving

Heckman, Nancy E.

194

Sorex rohweri sp. nov. (Mammalia, Soricidae) from northwestern North America  

Microsoft Academic Search

Sorex rohweri sp. nov. is described on the basis of a series of specimens from the Olympic Peninsula and adjacent western regions of Washington State, USA, and southwestern British Columbia, Canada. It has been misidentified as Sorex cinereus Kerr, 1792, which occurs in the Cascade Range in west-central Washington, in coastal British Columbia, and regions farther to the northeast. The

R. L. Rausch; Jean. E. Feagin; Virginia R. Rausch

2007-01-01

195

Acinetobacter sp. HM746599 isolated from leatherback turtle blood.  

PubMed

A newly described bacterial isolate, Acinetobacter sp. HM746599, has been obtained from leatherback sea turtle hatchling blood. The implication is that the hatchling was infected during development in the egg, which is substantiated by other studies to be reported by us in the future. The 16S rRNA gene sequence of the bacterium (GenBank accession number: HM746599) showed the greatest similarity to the identified species, Acinetobacter beijerinckii (97.6-99.78%) and Acinetobacter venetianus (99.78%). Acinetobacter sp. HM746599 are gram-negative, rod-shaped coccobacilli and are hemolytic/cytotoxic to human and sea turtle red blood cells (RBCs). Hemolysis is not the result of any detectable soluble toxin. Acinetobacter beijerinckii and A. venetianus hemolyze sheep RBCs while Acinetobacter sp. HM746599 does not, and unlike A. venetianus, the growth of Acinetobacter sp. HM746599 and A. beijerinckii is not supported by l-arginine. Many Acinetobacter species, especially hemolytic ones, are pathogenic to immunologically compromised humans and it is possible that, in addition to sea turtles, this bacterium might also be a danger to susceptible humans who handle infected hatchlings. The bacteria are available from CCUG (Culture Collection, University Gothenburg, Göteborg, Sweden) and from NRRL (Agricultural Research Service Culture Collection, Peoria, IL). PMID:21707734

Soslau, Gerald; Russell, Jacob A; Spotila, James R; Mathew, Andrew J; Bagsiyao, Pamela

2011-09-01

196

SUPPORTING INFORMATION Synthesis of Highly Crystalline sp2  

E-print Network

1 SUPPORTING INFORMATION Synthesis of Highly Crystalline sp2 -Bonded Boron Nitride Aerogels Michael characterization (XRD and nitrogen adsorption isotherms) of the BN aerogels discussed in the text, as well as a schematic detailing the crucible in which the synthesis of the aerogels is performed. #12;2 Figure S1

Zettl, Alex

197

Antibacterial Secondary Metabolites from the Cave Sponge Xestospongia sp  

PubMed Central

Chemical investigation of the cave sponge Xestospongia sp. resulted in the isolation of three new polyacetylenic long chain compounds along with two known metabolites. The structures of the new metabolites were established by NMR and MS analyses. The antibacterial activity of the new metabolites was also evaluated. PMID:22822355

Ankisetty, Sridevi; Slattery, Marc

2012-01-01

198

Production of Thermostable ? Amylase and Cellulase from Cellulomonas sp  

Microsoft Academic Search

A bacterium, isolated from rabbit's waste and identified as Cellulomonas sp., had cellulase and thermostable ? -amylase activity when grown on wheat bran. Maximum activity of thermostable ? -amylase was obtained by adding 3% soluble starch. However, soybean oil (1 ml l - 1 ) could increase the production of ? -amylase and cellulase in wheat bran. The ? -amylase

G. EMTIAZI; I. NAHVI

2004-01-01

199

Uranium accumulation by immobilized cells of a Citrobacter sp  

Microsoft Academic Search

Uranium was removed from challenge flows presented to immobilized cells of aCitrobacter sp. In excess of 90% of the presented metal was recovered, giving high yields of accumulated metal which could be subsequently released from the immobilized cellsin situ.

Lynne E. Macaskie; A. C. R. Dean

1985-01-01

200

NASA/SP-2007-6105 Systems Engineering  

E-print Network

NASA/SP-2007-6105 Rev1 NASA Systems Engineering Handbook #12;NASA STI Program...in Profile Since its founding, the National Aeronautics and Space Administration (NASA) has been dedicated to the ad- vancement of aeronautics and space science. The NASA Scientific and Technical Information (STI) program

Rhoads, James

201

Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter  

E-print Network

Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter Na-Ri Shin, Mi , was isolated from a Korean alcohol fermentation starter. Optimal growth occurred at 37 6C, at pH 8 and in 1 was isolated from nuruk, which is an alcohol fermentation starter used as an amylolytic substance

Bae, Jin-Woo

202

Die Aufnahme partikulärer Nahrung bei Reniera sp. (Porifera)  

Microsoft Academic Search

The choanocyte chambers of the marine spongeReniera sp. protrude with their curved outer surface free into the incurrent canals. The water is sucked into the chambers by cavities between the choanocytes. Particles up to 1 µm in diameter may enter the chambers with the water current. These particles are trapped on the outer surface of the choanocyte collars and are

Paul-Friedrich Langenbruch

1985-01-01

203

Die Aufnahme partikulärer Nahrung bei Reniera sp. (Porifera)  

Microsoft Academic Search

The choanocyte chambers of the marine sponge Reniera sp. protrude with their curved outer surface free into the incurrent canals. The water is sucked into the chambers by cavities between the choanocytes. Particles up to 1 µm in diameter may enter the chambers with the water current. These particles are trapped on the outer surface of the choanocyte collars and

Paul-Friedrich Langenbruch

1985-01-01

204

ORIGINAL PAPER Candida gelsemii sp. nov., a yeast  

E-print Network

ORIGINAL PAPER Candida gelsemii sp. nov., a yeast of the Metschnikowiaceae clade isolated from+Business Media B.V. 2006 Abstract A new yeast species, Candida gelsemii, is described to accommodate three of Metschnikowia and Candida species known to occur in association with nectars and bees, as well as marine inverte

Thomson, James D.

205

Bohemamines from a marine-derived Streptomyces sp.  

PubMed

Investigation of the culture extracts of a marine-derived Streptomyces sp. led to the isolation of three new bohemamine-type pyrrolizidine alkaloids, bohemamine B (1), bohemamine C (2), and 5-chlorobohemamine C (3). The structures were elucidated using NMR methods, and the relative stereochemistry was determined using double-pulsed-field-gradient spin echo (DPFGSE) NOE studies. PMID:17125235

Bugni, Tim S; Woolery, Matthew; Kauffman, Christopher A; Jensen, Paul R; Fenical, William

2006-11-01

206

Increased Constituent Ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. and a Decrease in Microflora Diversity May Be Indicators of Ventilator-Associated Pneumonia: A Prospective Study in the Respiratory Tracts of Neonates  

PubMed Central

Ventilator-associated pneumonia (VAP) is a common complication and cause of death in neonates on mechanical ventilation. However, it is difficult to define the causes of VAP. To understand the causes of VAP, we undertook a prospective study based on the diversity of the microflora in VAP. The experimental group consisted of newborns who suffered from respiratory distress syndrome (RDS) and VAP, while the control group suffered from RDS without VAP. Sputa were collected within 1, 3, and 5 days of ventilation and were divided into six groups. DNA was extracted from the samples, and the 16S rDNA was PCR amplified, separated using denaturing gradient gel electrophoresis (DGGE), cloned and sequenced. The resulting sequences were compared using BLAST. The DGGE pictures were measured, and the richness, Shannon-Wiener index, and cluster maps were analyzed. No differences were found regarding the constituent ratio of any genus between the Non-VAP and VAP group within 1 day after intubation. After 1 to 3 days, the constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the VAP group were higher than those in the Non-VAP group, and the ratios of Serratia sp. and Achromobacter sp. were lower. After 3 to 5 days, the ratios of Klebsiella sp., Acinetobacter sp., Serratia sp., and Achromobacter sp. were lower than those in the Non-VAP group. The richness and Shannon-Wiener index of the Non-VAP group were higher than those of the VAP group from 1 to 3 days after intubation, while no differences were found within 1 day and from 3 to 5 days. We conclude that during the first three days of intubation, the microflora diversity in the lower respiratory tract was reduced due to VAP, and the greater constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the sputum may be indicators of VAP. PMID:24586277

Lu, Wei; Yu, Jialin; Ai, Qing; Liu, Dong; Song, Chao; Li, Luquan

2014-01-01

207

Biosorption characteristics of copper (II), chromium (III), nickel (II), and lead (II) from aqueous solutions by Chara sp. and Cladophora sp.  

PubMed

The aim of this research was to expose individual removals of copper, chromium, nickel, and lead from aqueous solutions via biosorption using nonliving algae species, Chara sp. and Cladophora sp. Optimum pH values for biosorption of copper (II), chromium (III), nickel (II), and lead (II) from aqueous solutions were determined to be 6, 7, 7, and 3 for Cladophora sp. and 5, 3, 5, and 4 for Chara sp. respectively. Maximum adsorption capacities of Chara sp. [10.54 for chromium (III) and 61.72 for lead (II)] and Cladophora sp. [6.59 for chromium (III) and 16.75 and 23.25 for lead (II)] for chromium (III) and lead (II) are similar. On the other hand, copper (II) and nickel (II) biosorption capacity of Cladophora sp. [14.28 for copper (II) and 16.75 for nickel (II)] is greater than Chara sp. [6.506 for copper (II) and 11.76 for nickel (II)]. Significantly high correlation coefficients indicated for the Langmuir adsorption isotherm models can be used to describe the equilibrium behavior of copper, chromium, nickel, and lead adsorption onto Cladophora sp. and Chara sp. PMID:17910369

Elmaci, Ay?e; Yonar, Taner; Ozengin, Nihan

2007-09-01

208

76 FR 73483 - Airworthiness Directives; Gulfstream Aerospace Corporation Model GV and GV-SP Airplanes  

Federal Register 2010, 2011, 2012, 2013

...Aerospace Corporation Model GV and GV-SP Airplanes AGENCY: Federal Aviation Administration...Aerospace Corporation Model GV and GV-SP airplanes. This AD was prompted by notification from the airplane manufacturer that the third fire...

2011-11-29

209

Forecasting the S&P 500 index using time series analysis and simulation methods  

E-print Network

The S&P 500 represents a diverse pool of securities in addition to Large Caps. A range of audiences are interested in the S&P 500 forecasts including investors, speculators, economists, government and researchers. The ...

Chan, Eric Glenn

2009-01-01

210

Proteome analysis of Pseudomonas sp. K82 biodegradation pathways.  

PubMed

Pseudomonas sp. K82 is a soil bacterium that can degrade and use monocyclic aromatic compounds including aniline, 3-methylaniline, 4-methylaniline, benzoate and p-hydroxybenzoate as its sole carbon and energy sources. In order to understand the impact of these aromatic compounds on metabolic pathways in Pseudomonas sp. K82, proteomes obtained from cultures exposed to different substrates were displayed by two-dimensional gel electrophoresis and were compared to search for differentially induced metabolic enzymes. Column separations of active fractions were performed to identify major biodegradation enzymes. More than thirty proteins involved in biodegradation and other types of metabolism were identified by electrospray ionization-quadrupole time of flight mass spectrometry. The proteome analysis suggested that Pseudomonas sp. K82 has three main metabolic pathways to degrade these aromatic compounds and induces specific metabolic pathways for each compound. The catechol 2,3-dioxygenase (CD2,3) pathway was the major pathway and the catechol 1,2-dioxygenase (beta-ketoadipate) pathway was the secondary pathway induced by aniline (aniline analogues) exposure. On the other hand, the catechol 1,2-dioxygenase pathway was the major pathway induced by benzoate exposure. For the degradation of p-hydroxybenzoate, the protocatechuate 4,5-dioxygenase pathway was the major degradation pathway induced. The nuclear magnetic resonance analysis of substrates demonstrated that Pseudomonas sp. K82 metabolizes some aromatic compounds more rapidly than others (benzoate > p-hydroxybenzoate > aniline) and that when combined, p-hydroxybenzoate metabolism is repressed by the presence of benzoate or aniline. These results suggest that proteome analysis can be useful in the high throughput study of bacterial metabolic pathways, including that of biodegradation, and that inter-relationships exist with respect to the metabolic pathways of aromatic compounds in Pseudomonas sp. K82. PMID:15449373

Kim, Seung Il; Kim, Jin Young; Yun, Sung-Ho; Kim, Jung-Hyun; Leem, Sun-Hee; Lee, Chulhyun

2004-11-01

211

Mixibius parvus sp. nov. and Diphascon (Diphascon) ziliense sp. nov., two new species of Eutardigrada from Sicily.  

PubMed

Two new species, Mixibius parvus sp. nov. and Diphascon (Diphascon) ziliense sp. nov. are described from Sicily. Mixibius parvus sp. nov. has three macroplacoids and a microplacoid and differs from M. tibetanus, the only other known species of the genus with those characteristics, in having a wrinkled cuticle without true small tubercles, a shorter microplacoid, smaller claw pt index values, and in lacking a cuticular bar on the first three pairs of the legs.Diphascon (Diphascon) ziliense sp. nov. lacks eye spots, has a pharyngeal bulb with two macroplacoids and a septulum, and possesses lunules and cuticular bars on the legs. It is similar to D. (D.) ramazzottii and D. (D.) procerum but differs from them in characters of the cuticular ornamentation and, in addition, from D. (D.) ramazzottii in having lunules and slightly longer claws in proportion to the body size and to the buccal tube. The new species differs from D. (D.) procerum in having stouter claws with a wider common portion and with the main branches shorter in proportion to the total length of the respective claws. PMID:24871024

Lisi, Oscar; Sabella, Giorgio; Pilato, Giovanni

2014-01-01

212

Short title: Ambrosiella roeperi sp. nov. Ambrosiella roeperi sp. nov. is the mycangial symbiont of the granulate ambrosia beetle,  

E-print Network

of the granulate ambrosia beetle, Xylosandrus crassiusculus Thomas C. Harrington Douglas McNew Chase Mayers the granulate ambrosia beetle, Xylosandrus crassiusculus (Coleoptera: Curculionidae: Scolytinae: Xyleborini of ambrosia beetles and closely related to Ceratocystis spp. Ambrosiella roeperi sp. nov. produce sporodochia

Harrington, Thomas C.

213

Hydrolysis of surimi wastewater for production of transglutaminase by Enterobacter sp. C2361 and Providencia sp. C1112.  

PubMed

Surimi wastewater (SWW) is an industrial wastewater, released during the washing step of surimi preparation from minced fish, that causes environmental problem. In this study, SWW produced from ornate threadfin bream (Nemipterus hexodon) was hydrolysed and used to cultivate Enterobacter sp. C2361 and Providencia sp. C1112 for the production of microbial transglutaminase (MTGase, EC 2.3.2.13). The SWW was repeatedly used to wash the fish mince that gained a final protein content of 3.20% (w/v). The commercial protease, Delvolase was the most appropriate protease used to produce fish protein hydrolysate (FPH) from SWW. The FPH at 40% degree of hydrolysis was used instead of a peptone portion in the SPY medium (3.0% starch, 2.0% peptone, 0.2% yeast extract, 0.2% MgSO(4), 0.2% K(2)HPO(4) and 0.2% KH(2)HPO(4), pH 7.0) to cultivate the tested strains at 37°C, shaking speed at 150rpm. Providencia sp. C1112 produced higher MTGase activity (1.78±0.05U/ml) than Streptoverticillium mobaraense (1.61±0.02U/ml) at 18h of cultivation in FPH medium. On the other hand, the Enterobacter sp. C2361 produced lower MTGase activity (1.18±0.03U/ml). PMID:22953841

H-Kittikun, Aran; Bourneow, Chaiwut; Benjakul, Soottawat

2012-12-01

214

A Novel SP1/SP3 Dependent Intronic Enhancer Governing Transcription of the UCP3 Gene in Brown Adipocytes  

PubMed Central

Uncoupling protein (UCP) 3 is a mitochondrial inner membrane protein implicated in lipid handling and metabolism of reactive oxygen species. Its transcription is mainly regulated by peroxisome proliferator-activated receptors (PPAR), a family of nuclear hormone receptors. Employing bandshift assays, RNA interference and reporter gene assays we examine an intronic region in the UCP3 gene harboring a cis-element essential for expression in brown adipocytes. We demonstrate binding of SP1 and SP3 to this element which is adjacent to a direct repeat 1 element mediating activation of UCP3 expression by PPAR? agonists. Transactivation mediated by these elements is interdependent and indispensable for UCP3 expression. Systematic deletion uncovered a third binding element, a putative NF1 site, in close proximity to the SP1/3 and PPAR? binding elements. Data mining demonstrated binding of MyoD and Myogenin to this third element in C2C12 cells, and, furthermore, revealed recruitment of p300. Taken together, this intronic region is the main enhancer driving UCP3 expression with SP1/3 and PPAR? as the core factors required for expression. PMID:24391766

Hoffmann, Christoph; Zimmermann, Anika; Hinney, Anke; Volckmar, Anna-Lena; Jarrett, Harry W.; Fromme, Tobias; Klingenspor, Martin

2013-01-01

215

Protein modeling and molecular dynamics simulation of the two novel surfactant proteins SP-G and SP-H.  

PubMed

Surfactant proteins are well known from the human lung where they are responsible for the stability and flexibility of the pulmonary surfactant system. They are able to influence the surface tension of the gas-liquid interface specifically by directly interacting with single lipids. This work describes the generation of reliable protein structure models to support the experimental characterization of two novel putative surfactant proteins called SP-G and SP-H. The obtained protein models were complemented by predicted posttranslational modifications and placed in a lipid model system mimicking the pulmonary surface. Molecular dynamics simulations of these protein-lipid systems showed the stability of the protein models and the formation of interactions between protein surface and lipid head groups on an atomic scale. Thereby, interaction interface and strength seem to be dependent on orientation and posttranslational modification of the protein. The here presented modeling was fundamental for experimental localization studies and the simulations showed that SP-G and SP-H are theoretically able to interact with lipid systems and thus are members of the surfactant protein family. PMID:25381619

Rausch, Felix; Schicht, Martin; Bräuer, Lars; Paulsen, Friedrich; Brandt, Wolfgang

2014-11-01

216

INTERACTION BETWEEN AN AMMONIUM-OXIDIZER, 'NITROSOMONAS' SP., AND TWO HETEROTROPHIC BACTERIA, 'NOCARDIA ATLANTICA' AND 'PSEUDOMONAS' SP.: A NOTE  

EPA Science Inventory

Closed culture experiments showed that the ability of an estuarine chemolithotrophic ammonium-oxidizer, Nitrosomonas sp., to convert ammonium to nitrite was substantially increased by as much as 150% and 50% when grown in the presence of two heterotrophs isolated from the same en...

217

Digenetic trematodes, Acanthatrium sp. and Lecithodendrium sp., as vectors of Neorickettsia risticii, the agent of Potomac horse fever.  

PubMed

Neorickettsia (formerly Ehrlichia) risticii, the agent of Potomac horse fever (PHF), has been recently detected in trematode stages found in the secretions of freshwater snails and in aquatic insects. Insectivores, such as bats and birds, may serve as the definitive host of the trematode vector. To determine the definitive helminth vector, five bats (Myotis yumanensis) and three swallows (Hirundo rustica, Tachycineta bicolor) were collected from a PHF endemic location in northern California. Bats and swallows were dissected and their major organs examined for trematodes and for N. risticii DNA using a nested polymerase chain reaction (PCR) assay. Adult digenetic trematodes, Acanthatrium sp. and/or Lecithodendrium sp., were recovered from the gastrointestinal tract of all bats and from one swallow. The intestine of three bats, the spleen of two bats and one swallow as well as the liver of one swallow tested PCR positive for N. risticii. From a total of seven pools of identical digenetic trematodes collected from single hosts, two pools of Acanthatrium sp. and one pool of Lecithodendrium sp. tested PCR positive. The results of this investigation provide preliminary evidence that at least two trematodes in the family Lecithodendriidae are vectors of N. risticii. The data also suggest that bats and swallows not only act as a host for trematodes but also as a possible natural reservoir for N. risticii. PMID:14627451

Pusterla, N; Johnson, E M; Chae, J S; Madigan, J E

2003-12-01

218

Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations  

Microsoft Academic Search

Two strains of Acetobacter sp., LTH 2460T and LTH 2458T, have been isolated from running red wine and cider vinegar fermentations, respectively. Taxonomic characteristics of the isolates were investigated. Comparative analysis of the 165 rRNA sequences revealed > 99% similarity between strain LTH 2460T and the type strains of the related species Acetobacter europaeus and Acetobacter xylinus and between strain

Stephan J. Sokollek; C. Hertel; Walter P. Hammes

1998-01-01

219

Leiothecium cristatum sp. nov. and Aspergillus posadasensis sp. nov., two species of Eurotiales from rainforest soils in South America.  

PubMed

We describe two novel fungi isolated from soil samples collected in Northern Argentina and belonging to the family Aspergillaceae of the order Eurotiales: Leiothecium cristatum sp. nov. and Aspergillus posadasensis sp. nov. Leiothecium cristatum sp. nov., represented by the ex-type strain FMR 11998(T) (?=?CBS 134260(T)?=?NBRC 109843(T)), is distinguishable morphologically from the type species of the genus, Leiothecium ellipsoideum, by the presence of irregular reticulate ascospores with two prominent equatorial crests, and Aspergillus posadasensis sp. nov., represented by the ex-type strain FMR 12168(T) (?=?CBS 134259(T)?=?NBRC 109845(T)), is differentiated from Aspergillus acanthosporus, the nearest species phylogenetically, by its non-sclerotioid ascomata and a lack of an asexual stage on all culture media tested. The taxonomic proposals are supported by the analysis of the sequences of the internal transcribed spacer region, the D1-D2 domains of the 28S rRNA gene, the fragments of the RNA polymerase II largest subunit, and the putative chaperonin complex related to TCP-1, ?-tubulin and calmodulin genes. PMID:24871778

Marin-Felix, Yasmina; Cano-Lira, José Francisco; Guarro, Josep; Stchigel, Alberto Miguel

2014-08-01

220

Characterization of a Streptococcus sp.-Veillonella sp. Community Micromanipulated from Dental Plaque?  

PubMed Central

Streptococci and veillonellae occur in mixed-species colonies during formation of early dental plaque. One factor hypothesized to be important in assembly of these initial communities is coaggregation (cell-cell recognition by genetically distinct bacteria). Intrageneric coaggregation of streptococci occurs when a lectin-like adhesin on one streptococcal species recognizes a receptor polysaccharide (RPS) on the partner species. Veillonellae also coaggregate with streptococci. These genera interact metabolically; lactic acid produced by streptococci is a carbon source for veillonellae. To transpose these interactions from undisturbed dental plaque to an experimentally tractable in vitro biofilm model, a community consisting of RPS-bearing streptococci juxtaposed with veillonellae was targeted by quantum dot-based immunofluorescence and then micromanipulated off the enamel surface and cultured. Besides the expected antibody-reactive cell types, a non-antibody-reactive streptococcus invisible during micromanipulation was obtained. The streptococci were identified as Streptococcus oralis (RPS bearing) and Streptococcus gordonii (adhesin bearing). The veillonellae could not be cultivated; however, a veillonella 16S rRNA gene sequence was amplified from the original isolation mixture, and this sequence was identical to the sequence of the previously studied organism Veillonella sp. strain PK1910, an oral isolate in our culture collection. S. oralis coaggregated with S. gordonii by an RPS-dependent mechanism, and both streptococci coaggregated with PK1910, which was used as a surrogate during in vitro community reconstruction. The streptococci and strain PK1910 formed interdigitated three-species clusters when grown as a biofilm using saliva as the nutritional source. PK1910 grew only when streptococci were present. This study confirms that RPS-mediated intrageneric coaggregation occurs in the earliest stages of plaque formation by bringing bacteria together to create a functional community. PMID:18805978

Chalmers, Natalia I.; Palmer, Robert J.; Cisar, John O.; Kolenbrander, Paul E.

2008-01-01

221

Nucleolin enhances internal ribosomal entry site (IRES)-mediated translation of Sp1 in tumorigenesis.  

PubMed

Our previous study indicated that specificity protein-1 (Sp1) is accumulated during hypoxia in an internal ribosomal entry site (IRES)-dependent manner. Herein, we found that the Sp1 was induced strongly at the protein level, but not in the mRNA level, in lung tumor tissue, indicating that translational regulation might contribute to the Sp1 accumulation during tumorigenesis. A further study showed that the translation of Sp1 was dramatically induced through an IRES-dependent pathway. RNA immunoprecipitation analysis of proteins bound to the 5'-untranslated region (5'-UTR) of Sp1 identified interacting protein - nucleolin. Knockdown of nucleolin significantly inhibited IRES-mediated translation of Sp1, suggesting that nucleolin positively facilitates Sp1 IRES activation. Further analysis of the interaction between nucleolin and the 5'-UTR of Sp1 mRNA revealed that the GAR domain was important for IRES-mediated translation of Sp1. Moreover, gefitinib, and LY294002 and MK2206 compounds inhibited IRES-mediated Sp1 translation, implying that activation of the epithelial growth factor receptor (EGFR) pathway via Akt activation triggers the IRES pathway. In conclusion, EGFR activation-mediated nucleolin phosphorylated at Thr641 and Thr707 was recruited to the 5'-UTR of Sp1 as an IRES trans-acting factor to modulate Sp1 translation during lung cancer formation. PMID:25173817

Hung, Chia-Yang; Yang, Wen-Bin; Wang, Shao-An; Hsu, Tsung-I; Chang, Wen-Chang; Hung, Jan-Jong

2014-12-01

222

Biological control of wood decay in an open tropical environment with Penicillium sp. and Trichoderma viride  

Microsoft Academic Search

The ability of Penicillium sp. and Trichoderma viride to retard the decay of obeche (Triplochiton scleroxylon) wood in the field for 11 months (January–November) covering dry and wet seasons in a tropical environment was investigated using the ‘graveyard’ method. Inoculation of stakes with Gloeophyllum sp. or G. sepiarium (decay fungi) 24 h after treatment with T. viride or Penicillium sp.

B. O Ejechi

1997-01-01

223

Lutte biologique contre Polymyxa betae (Kes-kin) au moyen de Trichoderma sp. Rsultats  

E-print Network

NOTE Lutte biologique contre Polymyxa betae (Kes- kin) au moyen de Trichoderma sp. Résultats dans un sol naturelle- ment infesté par Polymyxa betae et traité par un inoculum de Trichoderma sp. 17 of Polymyxa betae (KeskinJ with Trichoderma sp.. Preliminary results in vivo. Seedlings of sugar, beet (cv

Paris-Sud XI, Université de

224

Structure and conformation of the disulfide bond in dimeric lung surfactant peptides SP-B 1–25 and SP-B 8–25  

Microsoft Academic Search

Raman spectroscopy was used to determine the conformation of the disulfide linkage between cysteine residues in the homodimeric construct of the N-terminal alpha helical domain of surfactant protein B (dSP-B1–25). The conformation of the disulfide bond between cysteine residues in position 8 of the homodimer of dSP-B1–25 was compared with that of a truncated homodimer (dSP-B8–25) of the peptide having

Nilanjana Biswas; Alan J. Waring; Frans J. Walther; Richard A. Dluhy

2007-01-01

225

Candida kashinagacola sp. nov., C. pseudovanderkliftii sp. nov. and C. vanderkliftii sp. nov., three new yeasts from ambrosia beetle-associated sources.  

PubMed

Three new yeast species, Candida kashinagacola (JCM 15019(T) = CBS 10903(T)), C. pseudovanderkliftii (JCM 15025(T) = CBS 10904(T)), and C. vanderkliftii (JCM 15029(T) = CBS 10905(T)) are described on the basis of comparison of nucleotide sequences of large subunit ribosomal DNA D1/D2 region (LSU rDNA D1/D2). The nearest assigned species of the three new species was Candida llanquihuensis. Candida kashinagacola and C. pseudovanderkliftii differed from C. llanquihuensis by 3.8% nucleotide substitution of the region, while C. vanderkliftii did by 4.4%. Three new species differed in a number of physiological and growth characteristics from any previously assigned species and from one another. A phylogenetic tree based on the sequences of LSU rDNA D1/D2 showed that these new species together with Candida sp. ST-246, Candida sp. JW01-7-11-1-4-y2, Candida sp. BG02-7-20-001A-2-1 and C. llanquihuensis form a clade near Ambrosiozyma species. The new species did not assimilate methanol as a sole source of carbon, which supported the monophyly of these non methanol-assimilating species which are closely related to the methylotrophic yeasts. Candida kashinagacola was frequently isolated from the beetle galleries of Platypus quercivorus in three different host trees (Quercus serrata, Q. laurifolia and Castanopsis cuspidata) located in the sourthern part of Kyoto, Japan, thus indicating that this species may be a primary ambrosia fungus of P. quercivorus. On the other hand, C. pseudovanderkliftii and C. vanderkliftii were isolated only from beetle galleries in Q. laurifolia. Candida vanderkliftii was isolated from beetle gallery of Platypus lewisi as well as those of P. quercivorus. Candida pseudovanderkliftii and C. vanderkliftii are assumed to be auxiliary ambrosia fungi of P. quercivorus. PMID:18537038

Endoh, Rikiya; Suzuki, Motofumi; Benno, Yoshimi; Futai, Kazuyoshi

2008-10-01

226

Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes.  

PubMed

Taxonomic studies were performed on 36 strains that were isolated from microbial mats in Antarctic lakes of the Vestfold Hills, the Larsemann Hills and the McMurdo Dry Valleys. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains are related to members of the genus Flavobacterium; sequence similarity values with their nearest phylogenetic neighbours ranged from 96.8 to 98.5%. Results of DNA-DNA hybridization and comparison of repetitive extragenic palindromic DNA-PCR fingerprinting patterns revealed that these strains are members of three distinct species. Genotypic results, together with phenotypic characteristics, allowed the differentiation of these species from related Flavobacterium species with validly published names. The isolates are Gram-negative, chemoheterotrophic, rod-shaped cells that are psychrophilic and moderately halotolerant; their DNA G+C contents range from 33.1 to 34.5 mol%. Their whole-cell fatty acid profiles are similar and include C(15:0), anteiso-C(15:0), iso-C(15:0), C(15:1)omega6c, iso-C(16:0), iso-C(16:0) 3-OH and summed feature 3 (which comprises iso-C(15:0) 2-OH, C(16:1)omega7c or both) as major fatty acid components. On the basis of these results, three novel species are proposed, namely Flavobacterium degerlachei sp. nov. (consisting of 14 strains, with LMG 21915T=DSM 15718T as the type strain), Flavobacterium micromati sp. nov. (consisting of three strains, with LMG 21919T=CIP 108161T as the type strain) and Flavobacterium frigoris sp. nov. (consisting of 19 strains, with LMG 21922T=DSM 15719T as the type strain). PMID:14742463

Van Trappen, Stefanie; Vandecandelaere, Ilse; Mergaert, Joris; Swings, Jean

2004-01-01

227

Identification of Acetobacter strains isolated from Indonesian sources, and proposals of Acetobacter syzygii sp. nov., Acetobacter cibinongensis sp. nov., and Acetobacter orientalis sp. nov.  

PubMed

Forty-six strains of acetic acid bacteria newly isolated from flowers, fruits, and fermented foods collected in Indonesia were taxonomically studied. They were Gram-negative rods, produced acetic acid from ethanol, oxidized acetate and lactate to CO(2) and H(2)O, and had Q-9 as the major ubiquinone system. On the basis of DNA-DNA similarity, all strains studied, including type strains and reference strains of the genus Acetobacter, were separated into eleven groups (Groups I to XI). Of the 46 isolates, two isolates were included in Group II and identified as Acetobacter pasteurianus, five in Group IV as A. orleanensis, 16 in Group V as A. lovaniensis, five in Group VII as A. indonesiensis, and three in Group VIII as A. tropicalis. The remaining 15 isolates constituted three new groups based on DNA-DNA similarity; four isolates were included in Group IX, two in Group X, and nine in Group XI. No isolates were identified as A. aceti (Group I), A. peroxydans (Group III), and A. estunensis (Group VI). Phylogenetic analysis based on 16S rDNA sequences of representative strains of the Groups indicated belonging to the strains of the genus Acetobacter. On the basis of DNA base composition, DNA-DNA similarity, and 16S rDNA sequences, three new species of the genus Acetobacter are proposed: Acetobacter syzygii sp. nov. for Group IX, Acetobacter cibinongensis sp. nov. for Group X, and Acetobacter orientalis sp. nov. for Group XI. The distribution of Acetobacter strains in Indonesia is discussed in light of isolation sources. PMID:12483554

Lisdiyanti, Puspita; Kawasaki, Hiroko; Seki, Tatsuji; Yamada, Yuzo; Uchimura, Tai; Komagata, Kazuo

2001-06-01

228

Cisplatin in 5% Ethanol Eradicates Cisplatin-Resistant Lung Tumor by Killing Lung Cancer Side Population (SP) Cells and Non-SP Cells  

PubMed Central

Cancer side population (SP) cells with cancer stem cell-like properties are thought to be responsible for lung cancer chemotherapy resistance and currently no drug can efficiently target them. Breast cancer resistance protein (BRCP/ABCG2) is a major drug transporter in protecting lung cancer SP cells from cytotoxic agents. We showed that a low concentration of ethanol, which inhibits many membrane proteins, inhibits ABCG2 in lung cancer SP cells. Furthermore, cytotoxic cisplatin (DDP) in 5% (vol/vol) ethanol kills SP plus non-SP cancer cells better than either treatment alone in eradicating chemoresistant lung tumors. We found that 5% ethanol did not reduce ABCG2 protein levels, but significantly reduced ABCG2 protein function by a Hoechst 33342 extrusion assay, an ATPase activity assay, and transmission electron microscopy. Further, DDP in 5% ethanol (5% ethanol–DDP) induced apoptosis of the SP plus non-SP cancer cells both in vitro and in vivo. In DDP-resistant A549/DDP lung tumor-bearing Balb/C nude mice, intratumoral injection of 5% ethanol–DDP regressed tumors and significantly improved survivals compared with 5% ethanol, DDP alone, or control. Intratumoral injection of 5% ethanol–DDP helped eradicate tumors in 30% (3/10) of the mice after 4 weeks treatment. By killing SP and non-SP cancer cells, 5% ethanol–DDP could eradicate DDP-resistant lung tumor and extend survival, providing a novel way to improve chemoresistant lung cancer survival for clinic. PMID:24009622

Niu, Qi; Wang, Wei; Li, Yong; Ruden, Douglas M.; Li, Qian; Wang, Fenghua

2013-01-01

229

Complete genome sequence of Paenibacillus sp. strain JDR-2  

SciTech Connect

Paenibacillus sp. strain JDR-2, an aggressively xylanolytic bacterium isolated from sweetgum (Liquidambar styraciflua) wood, is able to efficiently depolymerize, assimilate and metabolize 4-O-methylglucuronoxylan, the predominant structural component of hardwood hemicelluloses. A basis for this capability was first supported by the identification of genes and characterization of encoded enzymes and has been further defined by the sequencing and annotation of the complete genome, which we describe. In addition to genes implicated in the utilization of -1,4-xylan, genes have also been identified for the utilization of other hemicellulosic polysaccharides. The genome of Paenibacillus sp. JDR-2 contains 7,184,930 bp in a single replicon with 6,288 protein-coding and 122 RNA genes. Uniquely prominent are 874 genes encoding proteins involved in carbohydrate transport and metabolism. The prevalence and organization of these genes support a metabolic potential for bioprocessing of hemicellulose fractions derived from lignocellulosic resources.

Chow, Virginia [University of Florida; Nong, Guang [University of Florida; St. John, Franz J. [US Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA; Dickstein, Ellen [University of Florida; Chertkov, Olga [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Brettin, Thomas S [ORNL; Han, James [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Martin, Joel [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Jones, Jeffrey B. [University of Florida; Ingram, Lonnie O. [University of Florida; Shanmugam, Keelnathan T. [University of Florida; Preston, James F. [University of Florida

2012-01-01

230

Genome sequence and description of Corynebacterium ihumii sp. nov.  

PubMed Central

Corynebacterium ihumii strain GD7T sp. nov. is proposed as the type strain of a new species, which belongs to the family Corynebacteriaceae of the class Actinobacteria. This strain was isolated from the fecal flora of a 62 year-old male patient, as a part of the culturomics study. Corynebacterium ihumii is a Gram positive, facultativly anaerobic, nonsporulating bacillus. Here, we describe the features of this organism, together with the high quality draft genome sequence, annotation and the comparison with other member of the genus Corynebacteria. C. ihumii genome is 2,232,265 bp long (one chromosome but no plasmid) containing 2,125 protein-coding and 53 RNA genes, including 4 rRNA genes. The whole-genome shotgun sequence of Corynebacterium ihumii strain GD7T sp. nov has been deposited in EMBL under accession number GCA_000403725. PMID:25197488

Padmanabhan, Roshan; Dubourg, Gregory; Lagier, Jean-Christophe; Couderc, Carine; Michelle, Caroline; Raoult, Didier; Fournier, Pierre-Edouard

2014-01-01

231

Biosynthesis of Carotenoids in Brevibacterium sp. KY-43131  

PubMed Central

The biosynthesis of 4-keto and 4,4?-diketo carotenoids in Brevibacterium sp. KY-4313 was studied. Echinenone and canthaxanthin were isolated from the cultures grown on a medium containing several n-alkanes. When glutathione was added to the bacterial cultures, the formation of canthaxanthin was inhibited while ?-carotene and its hydroxy derivatives accumulated. It is suggested that these 4-hydroxy compounds, isocryptoxanthin, isozeaxanthin, and 4-hydroxy-4?-keto-?-carotene, are intermediates in the biosynthesis of canthaxanthin. In the presence of 2-(4-chlorophenylthio)-triethylamine hydrochloride or nicotine, lycopene and neurosporene accumulated. The ?-carotene level decreased slightly but ?-zeacarotene remained unchanged. ?-carotene and its derivatives were resynthesized upon removal of the inhibitors. It was concluded that cyclization can take place at either the neurosporene or lycopene level in Brevibacterium sp. KY-4313. PMID:4828305

Hsieh, L. K.; Lee, Tung-Ching; Chichester, C. O.; Simpson, K. L.

1974-01-01

232

Tongue adhesion in the horned frog Ceratophrys sp.  

NASA Astrophysics Data System (ADS)

Frogs are well-known to capture elusive prey with their protrusible and adhesive tongues. However, the adhesive performance of frog tongues and the mechanism of the contact formation with the prey item remain unknown. Here we measured for the first time adhesive forces and tongue contact areas in living individuals of a horned frog (Ceratophrys sp.) against glass. We found that Ceratophrys sp. generates adhesive forces well beyond its own body weight. Surprisingly, we found that the tongues adhered stronger in feeding trials in which the coverage of the tongue contact area with mucus was relatively low. Thus, besides the presence of mucus, other features of the frog tongue (surface profile, material properties) are important to generate sufficient adhesive forces. Overall, the experimental data shows that frog tongues can be best compared to pressure sensitive adhesives (PSAs) that are of common technical use as adhesive tapes or labels.

Kleinteich, Thomas; Gorb, Stanislav N.

2014-06-01

233

Complete genome sequence of Paenibacillus sp. strain JDR-2  

PubMed Central

Paenibacillus sp. strain JDR-2, an aggressively xylanolytic bacterium isolated from sweetgum (Liquidambar styraciflua) wood, is able to efficiently depolymerize, assimilate and metabolize 4-O-methylglucuronoxylan, the predominant structural component of hardwood hemicelluloses. A basis for this capability was first supported by the identification of genes and characterization of encoded enzymes and has been further defined by the sequencing and annotation of the complete genome, which we describe. In addition to genes implicated in the utilization of ?-1,4-xylan, genes have also been identified for the utilization of other hemicellulosic polysaccharides. The genome of Paenibacillus sp. JDR-2 contains 7,184,930 bp in a single replicon with 6,288 protein-coding and 122 RNA genes. Uniquely prominent are 874 genes encoding proteins involved in carbohydrate transport and metabolism. The prevalence and organization of these genes support a metabolic potential for bioprocessing of hemicellulose fractions derived from lignocellulosic resources. PMID:22675593

Chow, Virginia; Nong, Guang; St. John, Franz J.; Rice, John D.; Dickstein, Ellen; Chertkov, Olga; Bruce, David; Detter, Chris; Brettin, Thomas; Han, James; Woyke, Tanja; Pitluck, Sam; Nolan, Matt; Pati, Amrita; Martin, Joel; Copeland, Alex; Land, Miriam L.; Goodwin, Lynne; Jones, Jeffrey B.; Ingram, Lonnie O.; Shanmugam, Keelnathan T.; Preston, James F.

2012-01-01

234

Description of Goffartia phalacra n. sp. (Diplogastridae: Nematoda) from India  

PubMed Central

A new species, Goffartia phalacra n. sp. is described and illustrated. The body is thin and slender with L = 511 to 646 ?m; a = 37.1 to 47.4; b = 4.8 to 6; c = 2.6 to 4.8; c? = 13.6 to 32.8; V = 40% to 49% in females. Males are smaller but similar to females and the posterior region is strongly curved. The species is characterized by a tubular stoma, a smooth round lip region, anterior pharynx much smaller than posterior pharynx, two pairs of unicellular glands associated with the vagina, and males with a broad keel-shaped gubernaculum. G. phalacra n. sp. can be differentiated from all other species of the genus by its lip region and the structure of the gubernaculum. This is the first instance of a species of Goffartia occurring in a terrestrial habitat and the first report of a species from India. PMID:24987164

Singh, Gaurav K.; Yousuf, Gazala; Kumar, Puneet; Ahmad, Irfan

2014-01-01

235

Pseudovibrio ascidiaceicola sp. nov., isolated from ascidians (sea squirts).  

PubMed

Two bacterial strains, F423T and F10102, were isolated from two ascidians, Polycitor proliferus and Botryllidae sp., respectively, which were collected from a beach on the Boso peninsula in Japan. Cells of both isolates were motile, rod-shaped and formed star-shaped aggregates in the early stage of exponential growth, but were coccoid in stationary growth phase. The results of 16S rRNA gene sequence analysis, fatty acid analysis, DNA-DNA hybridization experiments and physiological and biochemical tests indicated that the two strains were members of a novel species of the genus Pseudovibrio for which the name Pseudovibrio ascidiaceicola sp. nov. is proposed. The type strain is F423T (=NBRC 100514T=IAM 15084T=DSM 16392T=KCTC 12308T). PMID:16449437

Fukunaga, Yukiyo; Kurahashi, Midori; Tanaka, Kenji; Yanagi, Kensuke; Yokota, Akira; Harayama, Shigeaki

2006-02-01

236

New Oxidized Zoanthamines from a Canary Islands Zoanthus sp.  

PubMed Central

Three new norzoanthamine-type alkaloids, named 2-hydroxy-11-ketonorzoanthamide B (1), norzoanthamide B (2) and 15-hydroxynorzoanthamine (3), were isolated from Zoanthus sp. specimens collected at the Canary Islands. Their structures were determined by interpretation of NMR and HR-ESIMS data. Relative configurations of their chiral centers were proposed on the basis of ROESY spectra and by comparison of their spectroscopic data with those of the well-known compound, norzoanthamine. PMID:25317536

Cen-Pacheco, Francisco; Norte Martin, Manuel; Fernandez, Jose Javier; Hernandez Daranas, Antonio

2014-01-01

237

Properties of the hydantoinase from agrobacterium SP. IP I-671  

Microsoft Academic Search

Summary A hydantoin-hydrolyzing enzyme has been purified from an newly isolatedAgrobacterium sp. by procedures including ammonium sulfate fractionation and ion-exchange chromatography. Kinetic studies have demonstrated that this enzyme, which is strictlyd-selective and has a broad substrate specificity exhibits remarkable stability. Microbial bioconversion at 60°C and pH 10.0, allowed complete conversion of 30 g\\/L ofd,l 5-benzylhydantoin into thedN-carbamyl derivative of phenylalanine

Serge Runser; Eric Ohleyer

1990-01-01

238

Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity  

Microsoft Academic Search

Arthrobacter sp. lipase (ABL, MTCC no. 5125) is being recognized as an efficient enzyme for the resolution of drugs and their intermediates. The immobilization of ABL on various matrices for its enantioselectivity, stability, and reusability has been studied. Immobilization by covalent bonding on sepharose and silica afforded a maximum of 380 and 40 IU\\/g activity, respectively, whereas sol–gel entrapment provided a

Asha Chaubey; Rajinder Parshad; Surrinder Koul; Subhash C. Taneja; Ghulam N. Qazi

2006-01-01

239

Composition and accumulation of secondary carotenoids in Chlorococcum sp  

Microsoft Academic Search

A locally isolated Chlorococcum sp. could accumulate astaxanthin and its esters as secondary carotenoids. The secondary carotenoids\\u000a could reach a concentration of 5.2 mg g?1 d. wt, and were located in the cytoplasm and chloroplast as globules. Cells grew best at pH 8.0 and 30 °C, at which the growth\\u000a rate was about 0.066 h?1. Acidic condition (pH 5.5 and

D. H. Zhang; Y. K. Ng; S. M. Phang

1997-01-01

240

Homalomena propinqua (Araceae) is Pollinated by Parastasia sp. (Scarabaeidae)  

NSDL National Science Digital Library

Beetles are important pollinators in a lowland dipterocarp forest in Southeast Asia. Most of canopy and emergent dipterocarps are pollinated by beetles feeding on floral tissues. Some understory trees and forest floor herbs are pollinated by different types of beetles. Homalomena propinqua (Araceae) is pollinated by two specias of beetles. One of them, Parastasia sp. (Scarabaeidae), stays inside the spathe chamber and feeds on staminodes.

2004-03-09

241

Growth and flocculation of a marine photosynthetic bacterium Rhodovulum sp  

Microsoft Academic Search

A marine photosynthetic bacterium (PS88), identified as Rhodovulum sp., with flocculating ability was isolated from the sea sediment mud of a shrimp cultivation farm in Thailand. This bacterium\\u000a flocculated in glutamate\\/malate medium during aerobic dark or anaerobic light cultivation. The flocculating ability was enhanced\\u000a with the increase of NaCl concentration to 6% (w\\/v). When PS88 was grown in glutamate\\/malate medium

M. Watanabe; K. Sasaki; Y. Nakashimada; T. Kakizono; N. Noparatnaraporn; N. Nishio

1998-01-01

242

Heterocyst Morphogenesis and Gene Expression in Anabaena sp. PCC 7120  

E-print Network

Head of Department, U. Jackson McMahan August 2010 Major Subject: Microbiology iii ABSTRACT Heterocyst Morphogenesis and Gene Expression in Anabaena sp. PCC 7120. (August 2010) Rodrigo Andres Mella Herrera, B.S., Universidad Austral de... carrying a patS-gfp reporter grown in medium containing nitrate are composed of vegetative cells (A), and have undergone heterocyst development one day after transfer to medium without combined nitrogen (B). A patS mutant strain carrying the same patS...

Mella Herrera, Rodrigo Andres

2010-10-12

243

Physical properties of the DNA of bacteriophage SP50  

Microsoft Academic Search

The following properties of the DNA of B. subtilis phage SP50 were established: Molecular weight (in Daltons) 102×106 (sedimentation velocity) 97×106 (viscosity) 97×106 (contour lengths of electron micrographs) Base Composition (in % GC) 41.7 (chemical analysis) 44 (melting point) 44 (buoyant density) No unusual bases were observed. The complementary strands of the DNA can be separated. The phage DNA has

N. Biswal; A. K. Kleinschmidt; H. C. Spatz; T. A. Trautner

1967-01-01

244

Metal ion accumulation by immobilised cells of Brevibacterium sp  

Microsoft Academic Search

  This paper explores the use of an experimental system based on polyacrylamide-entrapped cells of Brevibacterium sp strain PBZ for the removal of metal ions from solutions. Experiments were performed in columns filled with the immobilised\\u000a cells and challenged with influents containing 20?mg L?1 of lead and 10?mg L?1 of cadmium. The cells were able to accumulate lead (about 40?mg g?1

D Di Simine; C Finoli; A Vecchio; V Andreoni

1998-01-01

245

Resistance to Hydrogen Peroxide in Textile Waste Water Bacillus sp  

Microsoft Academic Search

A possible mechanism of resistance to hydrogen peroxide (H2O2) was studied for Bacillus sp. previously isolated from textile waste water. The results suggest that catalase is involved in the resistance and survival of this bacteria in the presence of low and high concentrations of H2O2. Inducible and constitutive catalase for exponential cells accumulated in extracellular, cytosolic and periplasmic fractions, with

OLA M. GOMAA; KHALED S. H. AZAB

246

Phycocyanobilin:Ferredoxin Oxidoreductase of Anabaena sp. PCC 7120  

Microsoft Academic Search

In cyanobacteria, the biosynthesis of the phycobili- protein and phytochrome chromophore precursor phy- cocyanobilin is catalyzed by the ferredoxin-dependent enzyme phycocyanobilin:ferredoxin oxidoreductase (PcyA), which mediates an atypical four-electron reduc- tion of biliverdin IX. Here we describe the expression, affinity purification, and biochemical characterization of recombinant PcyA from Anabaena sp. PCC 7120. A monomeric protein with a native Mr of 30,400

Nicole Frankenberg; J. Clark Lagarias

247

XPS and XANES studies of uranium reduction by Clostridium sp  

Microsoft Academic Search

Speciation of uranium in cultures of Clostridium sp. by X-ray absorption near-edge spectroscopy (XANES) at the National Synchrotron Light Source and by X-ray photoelectron spectroscopy (XPS) showed that U(VI) was reduced to U(IV). In addition to U(IV), a lower oxidation state of uranium, most probably U(III), was detected by XANES in the bacterial cultures. Reduction of uranium occurred only in

Arokiasamy J. Francis; Cleveland J. Dodge; Fulong Lu; Gary P. Halada; Clive R. Clayton

1994-01-01

248

Callyspongiolide, a cytotoxic macrolide from the marine sponge Callyspongia sp.  

PubMed

A novel macrolide, callyspongiolide, whose structure was determined by comprehensive analysis of the NMR and HRMS spectra, was isolated from the marine sponge Callyspongia sp. collected in Indonesia. The compound features a carbamate-substituted 14-membered macrocyclic lactone ring with a conjugated structurally unprecedented diene-ynic side chain terminating at a brominated benzene ring. Callyspongiolide showed strong cytotoxicity against human Jurkat J16 T and Ramos B lymphocytes. PMID:24329175

Pham, Cong-Dat; Hartmann, Rudolf; Böhler, Philip; Stork, Björn; Wesselborg, Sebastian; Lin, Wenhan; Lai, Daowan; Proksch, Peter

2014-01-01

249

Veraphis yoshitomii sp. n. (Coleoptera, Staphylinidae, Scydmaeninae) from Japan.  

PubMed

A new species of Eutheiini, Veraphis yoshitomii sp. n. is described from Shikoku, Japan. Diagnostic characters are discussed and key structures, including the aedeagus, are illustrated. The new species belongs to the Veraphis japonicus species group, which includes five species distributed in mountains of Japan and five possibly closely related ones in the sub-Himalayan China. The distribution of Japanese Veraphis is summarized and discussed.  PMID:25283294

Ja?oszy?ski, Pawe?

2014-01-01

250

Cytotoxic Sesterterpenoids Isolated from the Marine Sponge Scalarispongia sp.  

PubMed

Eight scalarane sesterterpenoids, including four new compounds, were isolated from the marine sponge Scalarispongia sp. The structures of the new compounds were elucidated by 2D-NMR and HRMS analyses. All of the isolated compounds, with the exception of 16-O-deacetyl-12,16-epi-scalarolbutanolide, showed significant in vitro cytotoxicity (GI50 values down to 5.2 ?M) against six human cancer cell lines. PMID:25375188

Lee, Yeon-Ju; Lee, Jeong-Woo; Lee, Dong-Geun; Lee, Hyi-Seung; Kang, Jong Soon; Yun, Jieun

2014-01-01

251

Characterization of a kerationlytic metalloprotease from Bacillus sp. SCB-3  

Microsoft Academic Search

A keratinolytic protease-producing microorganism was isolated from soybean paste waste and was identified as a strain of Bacillus sp. The keratinase was purified by polyethylene glycol precipitation and two successive column chromatographies with DEAE-Toyopearl\\u000a 650C and Sephacryl S-200 HR. The purified enzyme had overall 11 purification folds with an 18% yield. The results of sodium\\u000a dodecyl sulfate polyacrylamide gel electrophoresis

H. Lee; D. B. Suh; J. H. Hwang; H. J. Suh

2002-01-01

252

Natural transformation of Thermotoga sp. strain RQ7  

PubMed Central

Background Thermotoga species are organisms of enormous interest from a biotechnological as well as evolutionary point of view. Genetic modifications of Thermotoga spp. are often desired in order to fully release their multifarious potentials. Effective transformation of recombinant DNA into these bacteria constitutes a critical step of such efforts. This study aims to establish natural competency in Thermotoga spp. and to provide a convenient method to transform these organisms. Results Foreign DNA was found to be relatively stable in the supernatant of a Thermotoga culture for up to 6 hours. Adding donor DNA to T. sp. strain RQ7 at its early exponential growth phase (OD600 0.18?~?0.20) resulted in direct acquisition of the DNA by the cells. Both T. neapolitana chromosomal DNA and Thermotoga-E. coli shuttle vectors effectively transformed T. sp. strain RQ7, rendering the cells resistance to kanamycin. The kan gene carried by the shuttle vector pDH10 was detected by PCR from the plasmid extract of the transformants, and the amplicons were verified by restriction digestions. A procedure for natural transformation of Thermotoga spp. was established and optimized. With the optimized method, T. sp. strain RQ7 sustained a transformation frequency in the order of 10-7 with both genomic and plasmid DNA. Conclusions T. sp. strain RQ7 cells are naturally transformable during their early exponential phase. They acquire DNA from both closely and distantly related species. Both chromosomal DNA and plasmid DNA serve as suitable substrates for transformation. Our findings lend a convenient technical tool for the genetic engineering of Thermotoga spp. PMID:24884561

2014-01-01

253

Purification of surfactant protein D (SP-D) from pooled amniotic fluid and bronchoalveolar lavage.  

PubMed

Surfactant protein SP-D is a multimeric collagenous lectin, called collectin. SP-D is a multifunctional, pattern recognition innate immune molecule, which binds in a calcium dependent manner to an array of carbohydrates and lipids, thus offering resistance to invading pathogens, allergen challenge, and pulmonary inflammation. SP-D is predominantly found in the endoplasmic reticulum of type 2 pneumocytes and in the secretory granules of Clara or non-ciliated bronchiolar cells. The highest expression of SP-D is observed in the distal airways and alveoli. There is also an extra pulmonary existence of SP-D. The common sources of native full-length human SP-D are bronchoalveolar lavage (BAL) washings from normal or preferably patients suffering from alveolar proteinosis who overproduce SP-D in the lungs. Amniotic fluid collected at the term during parturition is another reasonable source. Here, we describe a simple and rapid method of purifying native SP-D away from SP-A which is also present in the same source. We also describe procedures of expressing and purifying a recombinant fragment of human SP-D (rhSP-D) comprising trimeric neck and carbohydrate recognition domains that has been shown to have therapeutic effects in murine models of allergy and infection. PMID:24218267

Dodagatta-Marri, Eswari; Qaseem, Asif S; Karbani, Najmunisa; Tsolaki, Anthony G; Waters, Patrick; Madan, Taruna; Kishore, Uday

2014-01-01

254

Role of zinc finger structure in nuclear localization of transcription factor Sp1  

SciTech Connect

Transcription factor Sp1 is localized in the nucleus and regulates gene expression. Our previous study demonstrated that the carboxyl terminal region of Sp1 containing 3-zinc finger region as DNA binding domain can also serve as nuclear localization signal (NLS). However, the nuclear transport mechanism of Sp1 has not been well understood. In this study, we performed a gene expression study on mutant Sp1 genes causing a set of amino acid substitutions in zinc finger domains to elucidate nuclear import activity. Nuclear localization of the GFP-fused mutant Sp1 proteins bearing concomitant substitutions in the first and third zinc fingers was highly inhibited. These mutant Sp1 proteins had also lost the binding ability as to the GC box sequence. The results suggest that the overall tertiary structure formed by the three zinc fingers is essential for nuclear localization of Sp1 as well as dispersed basic amino acids within the zinc fingers region.

Ito, Tatsuo; Azumano, Makiko [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Uwatoko, Chisana [Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, 610-0395 (Japan); Itoh, Kohji [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan)], E-mail: kitoh@ph.tokushima-u.ac.jp; Kuwahara, Jun [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, 610-0395 (Japan)], E-mail: jkuwahar@dwc.doshisha.ac.jp

2009-02-27

255

The formation of sp3 bonding in compressed BN.  

PubMed

Attributed to their specific atomic bonding, the soft, graphite-like, hexagonal boron nitride (h-BN) and its superhard, diamond-like, cubic polymorph (c-BN) are important technological materials with a wide range of applications. At high pressure and temperature, h-BN can directly transform to a hexagonal close-packed polymorph (w-BN) that can be partially quenched after releasing pressure. Previous theoretical calculations and experimental measurements (primarily on quenched samples) provided substantial information on the transition, but left unsettled questions due to the lack of in situ characterization at high pressures. Using inelastic X-ray scattering to probe the boron and nitrogen near K-edge spectroscopy, here we report the first observation of the conversion process of boron and nitrogen sp(2)- and p-bonding to sp(3) and the directional nature of the sp(3) bonding. In combination with in situ X-ray diffraction probe, we have further clarified the structure transformation mechanism. The present archetypal example opens two enormous, element-specific, research areas on high-pressure bonding evolutions of boron and nitrogen; each of the two elements and their respective compounds have displayed a wealth of intriguing pressure-induced phenomena that result from bonding changes, including metallization, superconductivity, semiconductivity, polymerization and superhardness. PMID:14743214

Meng, Yue; Mao, Ho-Kwang; Eng, Peter J; Trainor, Thomas P; Newville, Matthew; Hu, Michael Y; Kao, Chichang; Shu, Jinfu; Hausermann, Daniel; Hemley, Russell J

2004-02-01

256

SP-100 reactor with Brayton conversion for lunar surface applications  

NASA Technical Reports Server (NTRS)

Examined here is the potential for integrating Brayton-cycle power conversion with the SP-100 reactor for lunar surface power system applications. Two designs were characterized and modeled. The first design integrates a 100-kWe SP-100 Brayton power system with a lunar lander. This system is intended to meet early lunar mission power needs while minimizing on-site installation requirements. Man-rated radiation protection is provided by an integral multilayer, cylindrical lithium hydride/tungsten (LiH/W) shield encircling the reactor vessel. Design emphasis is on ease of deployment, safety, and reliability, while utilizing relatively near-term technology. The second design combines Brayton conversion with the SP-100 reactor in a erectable 550-kWe powerplant concept intended to satisfy later-phase lunar base power requirements. This system capitalizes on experience gained from operating the initial 100-kWe module and incorporates some technology improvements. For this system, the reactor is emplaced in a lunar regolith excavation to provide man-rated shielding, and the Brayton engines and radiators are mounted on the lunar surface and extend radially from the central reactor. Design emphasis is on performance, safety, long life, and operational flexibility.

Mason, Lee S.; Rodriguez, Carlos D.; Mckissock, Barbara I.; Hanlon, James C.; Mansfield, Brian C.

1992-01-01

257

Molasses as fermentation substrate for levan production by Halomonas sp.  

PubMed

Levan is a homopolymer of fructose with many outstanding properties like high solubility in oil and water, strong adhesiveness, good biocompatibility, and film-forming ability. However, its industrial use has long been hampered by costly production processes which rely on mesophilic bacteria and plants. Recently, Halomonas sp. AAD6 halophilic bacteria were found to be the only extremophilic species producing levan at high titers in semi-chemical medium containing sucrose, and in this study, pretreated sugar beet molasses and starch molasses were both found to be feasible substitutes for sucrose. Five different pretreatment methods and their combinations were applied to both molasses types. Biomass and levan concentrations reached by the Halomonas sp. AAD6 cells cultivated on 30 g/L of pretreated beet molasses were 6.09 g dry cells/L and 12.4 g/L, respectively. When compared with literature, Halomonas sp. was found to stand out with its exceptionally high levan production yields on available fructose. Molecular characterization and monosaccharide composition studies confirmed levan-type fructan structure of the biopolymers. Rheological properties under different conditions pointed to the typical characteristics of low viscosity and pseudoplastic behaviors of the levan polymers. Moreover, levan polymer produced from molasses showed high biocompatibility and affinity with both cancerous and non-cancerous cell lines. PMID:21161209

Küçüka?ik, Faruk; Kazak, Hande; Güney, Dilvin; Finore, Ilaria; Poli, Annarita; Yenigün, Orhan; Nicolaus, Barbara; Oner, Ebru Toksoy

2011-03-01

258

Isolation and Characterization of a Cyclohexane-Metabolizing Xanthobacter sp  

PubMed Central

An unusual Xanthobacter sp., capable of independent growth on cyclohexane as the sole source of carbon and energy, has been isolated from soil by using classical enrichment techniques. The mean generation time for growth on cyclohexane was 6 h. The microorganism showed a limited ability to utilize hydrocarbons, with only alicyclic hydrocarbons closely related to cyclohexane supporting growth. Ultrastructural studies indicated the presence of electron-transparent vesicles in the cyclohexane-grown Xanthobacter sp., but the presence of complex intracytoplasmic membranes could not be identified. A soluble inducible enzyme capable of oxidizing cyclohexane was identified in cell extracts. This enzyme had a pH optimum of 6.5, an absolute specificity for NADPH, and a stoichiometric requirement for molecular O2 which was consistent with the formation of cyclohexanol. The enzyme showed no activity towards straight chain alkanes and only a limited activity towards unsaturated ring compounds. Enzymatic studies with cell extracts have indicated the main route of metabolism of cyclohexane by this Xanthobacter sp. to proceed via cyclohexane ? cyclohexanol ? cyclohexanone ? 1-oxa-2-oxocycloheptane (?-caprolactone) ? 6-hydroxyhexanoate (6-hydroxycaproate) ? ? adipic acid. Alternative routes involving initial double hydroxylation of the cyclohexane ring may operate fortuituously but are unlikely to represent major pathways for the dissimilation of cyclohexane by this microorganism. PMID:16346796

Trower, Michael K.; Buckland, R. Martin; Higgins, Raymond; Griffin, Martin

1985-01-01

259

Users guide to the Argonne SP scheduling system  

SciTech Connect

During the past five years scientists discovered that modern UNIX workstations connected with ethernet and fiber networks could provide enough computational performance to compete with the supercomputers of the day. As this concept became increasingly popular, the need for distributed queuing and scheduling systems became apparent. Today, supercomputers, such as Argonne National Laboratory`s IBM SP system, can provide more CPU and networking speed than can be obtained from these networks of workstations. These modern supercomputers look like clusters of workstations, however, so developers felt that the scheduling systems that were previously used on clusters of workstations should still apply. After trying to apply some of these scheduling systems to Argonne`s SP environment, it became obvious that these two computer environments have very different scheduling needs. Recognizing this need and realizing that no one has addressed it, we developed a new scheduling system. The approach taken in creating this system was unique in that user input and interaction were encouraged throughout the development process. Thus, a scheduler was built that actually worked the way the users wanted it to work. This document serves a dual purpose. It is both a user`s guide and an administrator`s guide for the ANL SP scheduling system. Look for revisions to this guide that will be appearing.

Lifka, D.A. [Argonne National Lab., IL (United States)]|[Illinois Inst. of Technology, IL (United States); Henderson, M.W.; Rayl, K. [Argonne National Lab., IL (United States)

1995-05-01

260

Alkaline protease from Thermoactinomyces sp. RS1 mitigates industrial pollution.  

PubMed

Proteases have found a wide application in the several industrial processes, such as laundry detergents, protein recovery or solubilization, prion degradation, meat tenderizations, and in bating of hides and skins in leather industries. But the main hurdle in industrial application of proteases is their economical production on a large scale. The present investigation aimed to exploit the locally available inexpensive agricultural and household wastes for alkaline protease production using Thermoactinomyces sp. RS1 via solid-state fermentation (SSF) technique. The alkaline enzyme is potentially useful as an additive in commercial detergents to mitigate pollution load due to extensive use of caustic soda-based detergents. Thermoactinomyces sp. RS1 showed good protease production under SSF conditions of 55 °C, pH 9, and 50 % moisture content with potato peels as solid substrate. The presented findings revealed that crude alkaline protease produced by Thermoactinomyces sp. RS1 via SSF is of potential application in silver recovery from used X-ray films. PMID:24122212

Verma, Amit; Ansari, Mohammad W; Anwar, Mohmmad S; Agrawal, Ruchi; Agrawal, Sanjeev

2014-05-01

261

Purification and characterization of chitinase from Paenibacillus sp. D1.  

PubMed

A 56.56-kDa extracellular chitinase from Paenibacillus sp. D1 was purified to 52.3-fold by ion exchange chromatography using SP Sepharose. Maximum enzyme activity was recorded at pH 5.0 and 50 °C. MALDI-LC-MS/MS analysis identified the purified enzyme as chitinase with 60% similarity to chitinase Chi55 of Paenibacillus ehimensis. The activation energy (E (a)) for chitin hydrolysis and temperature quotient (Q (10)) at optimum temperature was found to be 19.14 kJ/mol and 1.25, respectively. Determination of kinetic constants k (m), V (max), k (cat), and k (cat)/k (m) and thermodynamic parameters ?H*, ?S*, ?G*, ?G*(E-S), and ?G*(E-T) revealed high affinity of the enzyme for chitin. The enzyme exhibited higher stability in presence of commonly used protectant fungicides Captan, Carbendazim, and Mancozeb compared to control as reflected from the t (1/2) values suggesting its applicability in integrated pest management for control of soil-borne fungal phytopathogens. The order of stability of chitinase in presence of fungicides at 80 °C as revealed from t (1/2) values and thermodynamic parameters E (a(d)) (activation energy for irreversible deactivation), ?H*, ?G*, and ?S* was: Captan > Carbendazim > Mancozeb > control. The present study is the first report on thermodynamic and kinetic characterization of chitinase from Paenibacillus sp. D1. PMID:21049291

Singh, Anil Kumar; Chhatpar, Hari S

2011-05-01

262

Genomic structure and cloning of two transcript isoforms of human Sp8  

PubMed Central

Background The Specificity proteins (Sp) are a family of transcription factors that have three highly conserved zinc-fingers located towards the carboxy-terminal that bind GC-boxes and assist in the initiation of gene transcription. Human Sp1-7 genes have been characterized. Recently, the phenotype of Sp8 null mice has been described, being tailless and having severe truncation of both fore and hind limbs. They also have malformed brains with defective closure of the anterior and posterior neuropore during brain development. Results The human Sp8 gene is a three-exon gene that maps to 7p21.3, close to the related Sp4 gene. From an osteosarcoma cell line we cloned two transcript variants that use two different first exons and have a common second exon. One clone encodes a 508-residue protein, Sp8L (isoform 1) and the other a shorter 490-residue protein, Sp8S (isoform 2). These two isoforms are conserved being found also in mice and zebrafish. Analysis of the Sp8L protein sequence reveals an amino-terminal hydrophobic Sp-motif that is disrupted in Sp8S, a buttonhead box and three C2H2 zinc-fingers. Sp8 mRNA expression was detected in a wide range of tissues at a low level, with the highest levels being found in brain. Treatment of the murine pluripotent cell line C3H10T1/2 with 100 ng/mL BMP-2 induced Sp8 mRNA after 24 hours. Conclusions There is conservation of the two Sp8 protein isoforms between primates, rodents and fish, suggesting that the isoforms have differing roles in gene regulation. Sp8 may play a role in chondrogenic/osteoblastic differentiation in addition to its role in brain and limb development. PMID:15533246

Milona, Maria-athina; Gough, Julie E; Edgar, Alasdair J

2004-01-01

263

Feeding by heterotrophic dinoflagellates and ciliates on the free-living dinoflagellate Symbiodinium sp. (Clade E).  

PubMed

To investigate heterotrophic protists grazing on Symbiodinium sp., we tested whether the common heterotrophic dinoflagellates Gyrodinium dominans, Gyrodinium moestrupii, Gyrodinium spirale, Oblea rotundata, Oxyrrhis marina, and Polykrikos kofoidii and the ciliates Balanion sp. and Parastrombidinopsis sp. preyed on the free-living dinoflagellate Symbiodinium sp. (clade E). We measured the growth and ingestion rates of O. marina and G. dominans on Symbiodinium sp. as a function of prey concentration. Furthermore, we compared the results to those obtained for other algal prey species. In addition, we measured the growth and ingestion rates of other predators at single prey concentrations at which these rates of O. marina and G. dominans were saturated. All predators tested in the present study, except Balanion sp., preyed on Symbiodinium sp. The specific growth rates of O. marina and G. dominans on Symbiodinium sp. increased rapidly with increasing mean prey concentration < ca. 740-815 ng C/ml (7,400-8,150 cells/ml), but became saturated at higher concentrations. The maximum growth rates of O. marina and G. dominans on Symbiodinium sp. (0.87 and 0.61/d) were much higher than those of G. moestrupii and P. kofoidii (0.11 and 0.04/d). Symbiodinium sp. did not support positive growth of G. spirale, O. rotundata, and Parastrombidinopsis sp. However, the maximum ingestion rates of P. kofoidii and Parastrombidinopsis sp. (6.7-10.0 ng C/predator/d) were much higher than those of O. marina and G. dominans on Symbiodinium sp. (1.9-2.1 ng C/predator/d). The results of the present study suggest that Symbiodinium sp. may increase or maintain the populations of some predators. PMID:24102740

Jeong, Hae Jin; Lim, An Suk; Yoo, Yeong Du; Lee, Moo Joon; Lee, Kyung Ha; Jang, Tae Young; Lee, Kitack

2014-01-01

264

Two novel ascomycetous yeast species, Wickerhamomyces scolytoplatypi sp. nov. and Cyberlindnera xylebori sp. nov., isolated from ambrosia beetle galleries.  

PubMed

Thirteen strains of yeasts were isolated from ambrosia beetle galleries at several sites in Japan. Based on the morphological and biochemical characteristics and phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene of the yeasts, 10 strains were shown to represent a novel species of the genus Wickerhamomyces, described as Wickerhamomyces scolytoplatypi sp. nov. (type strain NBRC 11029(T) = CBS 12186(T)), and were closely related to Wickerhamomyces hampshirensis. The three other strains represented a novel species of the genus Cyberlindnera, described as Cyberlindnera xylebori sp. nov. (type strain NBRC 11048(T) = CBS 12187(T)), and were closely related to Cyberlindnera euphorbiiphila. It is suggested that these species are associated with ambrosia beetles and we consider ambrosia beetle galleries as good sources of novel yeasts. PMID:23667144

Ninomiya, Shinya; Mikata, Kozaburo; Kajimura, Hisashi; Kawasaki, Hiroko

2013-07-01

265

Paramaritremopsis solielangi n. sp. and Microphallus kinsellai n. sp. (Digenea: Microphallidae) parasites of Charadrii (Aves) of Belize (Central America).  

PubMed

The authors describe and illustrate two trematodes from Belize (Central America): Paramaritremopsis solielangi n. sp. from the small intestine of Arenaria interpres is characterised by a body length of 478 microm, two short and pre-acetabular caeca, part of the uterus in close association with the cirrus-sac and left caecum, vitelline glands in the shape of a horseshoe, a short pre-ovarian cirrus-sac containing a long, cylindrical, voluminous and unarmed cirrus (size when evaginated: 150 x 20-30 microm) and Microphallus kinsellai n. sp. from the caeca of Actitis macularia characterised by a body length of 370 microm and a phallus which is 30 microm in diameter and asymmetrical (basically a pad with a moderately developed accessory lobe) and a straight ejaculatory canal. Levinseniella carteretensis is another microphallid recovered from Arenaria interpres. The term of "phallus" is proposed to name the male copulatory organ which characterizes the Microphallinae. PMID:10966220

Canaris, A G; Deblock, S

2000-10-01

266

Inhibition of xyloglucanase from an alkalothermophilic Thermomonospora sp. by a peptidic aspartic protease inhibitor from Penicillium sp. VM24.  

PubMed

A bifunctional inhibitor from Penicillium sp VM24 causing inactivation of xyloglucanase from Thermomonospora sp and an aspartic protease from Aspergillus saitoi was identified. Steady state kinetics studies of xyloglucanase and the inhibitor revealed an irreversible, non-competitive, two-step inhibition mechanism with IC(50) and K(i) values of 780 and 500nM respectively. The interaction of o-phthalaldehyde (OPTA)-labeled xyloglucanase with the inhibitor revealed that the inhibitor binds to the active site of the enzyme. Far- and near-UV spectrophotometric analysis suggests that the conformational changes induced in xyloglucanase by the inhibitor may be due to irreversible denaturation of enzyme. The bifunctional inhibitor may have potential as a biocontrol agent for the protection of plants against phytopathogenic fungi. PMID:22940347

Menon, Vishnu; Rao, Mala

2012-11-01

267

Paramaritremopsis solielangi n. sp. et Microphallus kinsellai n. sp. (Digenea: Microphallidae) parasites de Charadrii (Aves) du Bélize (Amérique centrale)  

Microsoft Academic Search

The authors describe and illustrate two trematodes from Belize (Central America): Paramaritremopsis solielangi n. sp. from the small intestine of Arenaria interpres is characterised by a body length of 478 m, two short and pre-acetabular caeca, part of the uterus in close association with the cirrus-sac and left caecum, vitelline glands in the shape of a horseshoe, a short pre-ovarian cirrus-sac

Albert G. Canaris; Stéphane Deblock

2000-01-01

268

Lipid profile remodeling in response to nitrogen deprivation in the microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae).  

PubMed

Many species of microalgae produce greatly enhanced amounts of triacylglycerides (TAGs), the key product for biodiesel production, in response to specific environmental stresses. Improvement of TAG production by microalgae through optimization of growth regimes is of great interest. This relies on understanding microalgal lipid metabolism in relation to stress response in particular the deprivation of nutrients that can induce enhanced TAG synthesis. In this study, a detailed investigation of changes in lipid composition in Chlorella sp. and Nannochloropsis sp. in response to nitrogen deprivation (N-deprivation) was performed to provide novel mechanistic insights into the lipidome during stress. As expected, an increase in TAGs and an overall decrease in polar lipids were observed. However, while most membrane lipid classes (phosphoglycerolipids and glycolipids) were found to decrease, the non-nitrogen containing phosphatidylglycerol levels increased considerably in both algae from initially low levels. Of particular significance, it was observed that the acyl composition of TAGs in Nannochloropsis sp. remain relatively constant, whereas Chlorella sp. showed greater variability following N-deprivation. In both algae the overall fatty acid profiles of the polar lipid classes were largely unaffected by N-deprivation, suggesting a specific FA profile for each compartment is maintained to enable continued function despite considerable reductions in the amount of these lipids. The changes observed in the overall fatty acid profile were due primarily to the decrease in proportion of polar lipids to TAGs. This study provides the most detailed lipidomic information on two different microalgae with utility in biodiesel production and nutraceutical industries and proposes the mechanisms for this rearrangement. This research also highlights the usefulness of the latest MS-based approaches for microalgae lipid research. PMID:25171084

Martin, Gregory J O; Hill, David R A; Olmstead, Ian L D; Bergamin, Amanda; Shears, Melanie J; Dias, Daniel A; Kentish, Sandra E; Scales, Peter J; Botté, Cyrille Y; Callahan, Damien L

2014-01-01

269

Sulfurospirillum barnesii sp. nov. and Sulfurospirillum arsenophilum sp. nov., new members of the Sulfurospirillum clade of the ?-Proteobacteria  

USGS Publications Warehouse

Two strains of dissimilatory arsenate-reducing vibrio-shaped bacteria are assigned to the genus Sulfurospirillum. These two new species, Sulfurospirillum barnesii strain SES-3(T) and Sulfurospirillum arsenophilum strain MIT-13(T), in addition to Sulfurospirillum sp. SM-5, two strains of Sulfurospirillum deleyianum, and Sulfurospirillum arcachonense, form a distinct clade within the ?? subclass of the Proteobacteria based on 16S rRNA analysis.

Stolz, J.F.; Ellis, D.J.; Blum, J.S.; Ahmann, D.; Lovley, D.R.; Oremland, R.S.

1999-01-01

270

Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction.  

PubMed

Prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases and hemicellulases that could be useful in second-generation bioethanol production. In this work, cellulolytic bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. There was a predominance of Gram positive isolates that belonged to the phyla Proteobacteria and Firmicutes. Many primary isolates with cellulolytic activity were not pure cultures. From these consortia, isolation of pure constituents was attempted in order to test the hypothesis whether microbial consortia are needed for full degradation of complex substrates. Two isolates, CB1-2-A-5 and VG-4-A-2, were obtained as the pure constituents of CB1-2 and VG-4 consortia, respectively. Based on 16S RNA sequence, they could be classified as Variovorax paradoxus and Paenibacillus alvei. Noteworthy, only VG-4 consortium showed measurable xylan degrading capacity and signs of filter paper degradation. However, no xylan or filter paper degrading capacities were observed for the pure cultures isolated from it, suggesting that other members of this consortium were necessary for these hydrolyzing activities. Our results indicated that Paenibacillus sp. and Variovorax sp. as well as VG-4 consortium, might be a useful source of hydrolytic enzymes. Moreover, although Variovorax sp. had been previously identified in metagenomic studies of cellulolytic communities, this is the first report on the isolation and characterization of this microorganism as a cellulolytic genus. PMID:23301200

Ghio, Silvina; Lorenzo, Gonzalo Sabarís Di; Lia, Verónica; Talia, Paola; Cataldi, Angel; Grasso, Daniel; Campos, Eleonora

2012-01-01

271

Genomic organization of an intron-containing sperm protein 17 gene (Sp17-1) and an intronless pseudogene (Sp17-2) in humans: a new model  

Microsoft Academic Search

Sp17 was initially thought to be a sperm specific protein involved in the interaction of the spermatozoon with the oocyte's surrounding extracellular glycoprotein matrix. Recent reports, however, indicate that Sp17 expression is neither testis-specific nor is it exclusively used for binding to the zona pellucida of the oocyte. In this study, we provide comprehensive characterization of the genomic structure of

Rico Buchli; Ann De Jong; Dick L Robbins

2002-01-01

272

Synthesis of cyclic thioethers through tandem C(sp3)-S and C(sp2)-S bond formations from alpha,beta'-dichloro vinyl ketones.  

PubMed

The synthesis of 5- to 8-memebered cyclic thioethers 4 has been achieved through a simple two-step sequence. The present methodology utilizes the facile Friedel-Crafts acylation of terminal alkynes 1 with acid chlorides 2 followed by tandem C(sp(3))-S and C(sp(2))-S bond formations with NaSH.xH(2)O. PMID:18278938

Oh, Kyungsoo; Kim, Hyunjung; Cardelli, Francesco; Bwititi, Tamayi; Martynow, Anna M

2008-03-21

273

Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163  

PubMed Central

Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by 1H NMR. Ten known compounds, including angucycline, diketopiperazine and ?-carboline derivatives 1–10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes. PMID:24857962

Dashti, Yousef; Grkovic, Tanja; Abdelmohsen, Usama Ramadan; Hentschel, Ute; Quinn, Ronald J.

2014-01-01

274

Hydrophobic pulmonary surfactant proteins SP-B and SP-C induce pore formation in planar lipid membranes: evidence for proteolipid pores.  

PubMed

Pulmonary surfactant is a complex mixture of lipids and specific surfactant proteins, including the hydrophobic proteins SP-B and SP-C, in charge of stabilizing the respiratory surface of mammalian lungs. The combined action of both proteins is responsible for the proper structure and dynamics of membrane arrays in the pulmonary surfactant network that covers the respiratory surface. In this study, we explore the possibility that proteins SP-B and SP-C induce the permeabilization of phospholipid membranes via pore formation. To this end, electrophysiological measurements have been carried out in planar lipid membranes prepared with different lipid/protein mixtures. Our main result is that channel-like structures are detected in the presence of SP-B, SP-C, or the native mixture of both proteins. Current traces show a high variety of conductance states (from pS to nS) that are dependent both on the lipid composition and the applied potential. We also show that the type of host lipid crucially determines the ionic selectivity of the observed pores: the anionic selectivity observed in zwitterionic membranes is inverted to cationic selectivity in the presence of negatively charged lipids. All those results suggest that SP-B and SP-C proteins promote the formation of proteolipid channels in which lipid molecules are functionally involved. We propose that proteolipidic membrane-permeabilizing structures may have an important role to tune ionic and lipidic flows through the pulmonary surfactant membrane network at the alveolar surfaces. PMID:23332067

Parra, Elisa; Alcaraz, Antonio; Cruz, Antonio; Aguilella, Vicente M; Pérez-Gil, Jesús

2013-01-01

275

FeCl3-catalyzed oxidative allylation of sp2 and sp3 C-H bond adjacent to a nitrogen atom: easy access to homoallyl tertiary amines.  

PubMed

Oxidative allylation to sp(2)- and sp(3)-carbon attached to the nitrogen atom was accomplished. The alpha-allylation of tertiary amines was catalyzed by easily available hydrated iron(III) chloride in combination with air or aqueous (t)BuOOH. Remarkably, N-allyl- and N-propagyl-tethered tertiary amines were also allylated through this protocol. PMID:20446705

Kumaraswamy, Gullapalli; Murthy, Akula Narayana; Pitchaiah, Arigala

2010-06-01

276

Mild ArI-Catalyzed C(sp(2) )?H or C(sp(3) )?H Functionalization/C?O Formation: An Intriguing Catalyst-Controlled Selectivity Switch.  

PubMed

A tandem C(sp(2) )?H and C(sp(3) )?H functionalization/C?O bond formation catalyzed by iodine(III) reagents generated in situ has been developed. The method shows wide scope under mild conditions and exhibits an unprecedented selectivity profile that can be switched depending on the catalyst employed. PMID:25156610

Wang, Xueqiang; Gallardo-Donaire, Joan; Martin, Ruben

2014-10-01

277

Descriptions of Rhodopseudomonas parapalustris sp. nov., Rhodopseudomonas harwoodiae sp. nov. and Rhodopseudomonas pseudopalustris sp. nov., and emended description of Rhodopseudomonas palustris.  

PubMed

Four strains (JA310(T), JA531(T), JA447 and JA490) of red to reddish brown pigmented, rod-shaped, motile and budding phototrophic bacteria were isolated from soil and freshwater sediment samples from different geographical regions of India. All strains contained bacteriochlorophyll a and carotenoids of the spirilloxanthin series. The major cellular fatty acid of strains JA310(T) and JA531(T) was C(18:1)?7c, the quinone was Q-10 and polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminohopanoid and an unidentified aminolipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that all strains clustered with species of the genus Rhodopseudomonas in the class Alphaproteobacteria. Strains JA531(T), JA447 and JA490 were genotypically (>80% related based on DNA-DNA hybridization) and phenotypically closely related to each other and the three strains were distinct from strain JA310(T) (33% related). Furthermore, all four strains had less than 48% relatedness (DNA-DNA hybridization) with type strains of members of the genus Rhodopseudomonas, i.e. Rhodopseudomonas palustris ATCC 17001(T), Rhodopseudomonas faecalis JCM 11668(T) and Rhodopseudomonas rhenobacensis DSM 12706(T). The genomic DNA G+C contents of strains JA310(T) and JA531(T) were 63.8 and 62.4 mol%, respectively. On the basis of phenotypic, chemotaxonomic and molecular genetic evidence, it is proposed that strains JA310(T) (?=?NBRC 106083(T)?=?KCTC 5839(T)) and JA531(T) (?=?NBRC 107575(T)?=?KCTC 5841(T)) be classified as the type strains of two novel species of the genus Rhodopseudomonas, Rhodopseudomonas parapalustris sp. nov. and Rhodopseudomonas harwoodiae sp. nov., respectively. In addition, we propose that strain DSM 123(T) (?=?NBRC 100419(T)) represents a novel species, Rhodopseudomonas pseudopalustris sp. nov., since this strain differs genotypically and phenotypically from R. palustris ATCC 17001(T) and other members of the genus Rhodopseudomonas. An emended description of R. palustris is also provided. PMID:21986724

Ramana, V Venkata; Chakravarthy, S Kalyana; Raj, P Shalem; Kumar, B Vinay; Shobha, E; Ramaprasad, E V V; Sasikala, Ch; Ramana, Ch V

2012-08-01

278

Two viable three-dimensional carbon semiconductors with an entirely sp2 configuration.  

PubMed

Using the first-principles method, we investigate the energetic stability, dynamic stability and electronic properties of two viable three-dimensional (3D) carbon semiconductors with an entirely sp(2) configuration, sp(2)-diamond and cubic-graphite. Both allotropes are more stable than the previously proposed K4-carbon and T-carbon and were confirmed to be dynamically stable. Interestingly, sp(2)-diamond and cubic-graphite behave as semiconductors, which is contrary to previously proposed all-sp(2) metallic carbons. sp(2)-Diamond is a semiconductor with a direct band gap of 1.66 eV and cubic-graphite is an indirect semiconductor with a band gap of 2.89 eV. Further studies show that both sp(2)-diamond and cubic-graphite possess structural all-sp(2) configurations but not electronic sp(2) hybridizations. The very low densities and entirely sp(2) configurations of sp(2)-diamond and cubic-graphite can be potentially applied in hydrogen-storage, photocatalysts and molecular sieves. PMID:23187896

He, Chaoyu; Sun, Lizhong; Zhang, Chunxiao; Zhong, Jianxin

2013-01-14

279

Cigarette Smoke Induces MUC5AC Protein Expression through the Activation of Sp1*  

PubMed Central

Cigarette smoke (CS) exposure is associated with increased mucus production and chronic obstructive pulmonary disease (COPD). MUC5AC is the major inducible mucus gene in the airway. The purpose of this investigation was to elucidate the mechanisms of CS-induced activation of MUC5AC gene transcription. We observed that the region ?3724/?3224 of the MUC5AC promoter is critical for CS-induced gene transcriptional activity and that this region contains two Sp1 binding sites. Using a lung-relevant model, we observed that CS increased nuclear Sp1 protein expression. Consequently, CS exposure resulted in enhanced Sp1-DNA binding activity and Sp1 trans-activation. Co-transfection of the MUC5AC-luc reporter with Sp1 expression plasmids resulted in significantly increased MUC5AC-luc activity, whereas co-treatment with mithramycin A, a Sp1 inhibitor, abolished CS-induced MUC5AC promoter activity. Using mobility shift assay and chromatin immunoprecipitation, we demonstrated that two Sp1 binding sites in the MUC5AC promoter are functional and responsive to CS exposure. A mutation of either Sp1 binding site in the MUC5AC promoter significantly decreased CS-induced promoter activity. Together, these data indicate that CS induces MUC5AC gene transcription predominantly through increased Sp1 nuclear protein levels and increased Sp1 binding to its promoter region. PMID:22700966

Di, Y. Peter; Zhao, Jinming; Harper, Richart

2012-01-01

280

Stramenopile microorganisms associated with the massive coral Favia sp.  

PubMed

The surfaces of massive corals of the genus Favia from Eilat, Red Sea, and from Heron Island, Great Barrier Reef, are covered by a layer of eukaryotic microorganisms. These microorganisms are embedded in the coral mucus and tissue. In the Gulf of Eilat, the prevalence of corals covered by patches of eukaryotic microorganisms was positively correlated with a decrease in water temperatures (from 25-28 degrees C in the summer to 20-23 degrees C in winter). Comparisons carried out using transmission and scanning electron microscopy showed morphological similarities between the microorganisms from the two geographically distant reefs. The microorganisms found on and in the tissues were approximately 5-15 microm in diameter, surrounded by scales in their cell wall, contained a nucleus, and included unique auto-florescent coccoid bodies of approximately 1 mum. Such morphological characters suggested that these microorganisms are stramenopile protists and in particular thraustochytrids. Molecular analysis, carried out using specific primers for stramenopile 18S rRNA genes, revealed that 90% (111/123) of the clones in the gene libraries were from the Thraustochytriidae. The dominant genera in this family were Aplanochytrium sp., Thraustochytrium sp., and Labyrinthuloides sp. Ten stramenopile strains were isolated and cultured from the corals. Some strains showed > or =97% similarity to clones derived from libraries of mucus-associated microorganisms retrieved directly from these corals. Fatty acid characterization of one of the prevalent strains revealed a high percentage of polyunsaturated fatty acids, including omega-3. The possible association of these stramenopiles in the coral holobiont appeared to be a positive one. PMID:20236189

Siboni, Nachshon; Rasoulouniriana, Diana; Ben-Dov, Eitan; Kramarsky-Winter, Esti; Sivan, Alex; Loya, Yossi; Hoegh-Guldberg, Ove; Kushmaro, Ariel

2010-01-01

281

Multicenter evaluation of Neurelec Digisonic® SP cochlear implant reliability.  

PubMed

Over the past decade, the adoption of universal hearing screening in newborns has led to earlier detection of hearing problems and significant lowering of the age of first cochlear implantation. As a consequence, recipients are now expected to keep their cochlear implants (CIs) for a longer period of time. Comprehensive longitudinal information on CI reliability is essential for device choice. The aim of this study was to assess the reliability (in children and adults) of the latest generation of the Digisonic(®) SP CI launched in 2006 by Neurelec. Failure rate (FR) and cumulative survival rate (CSR) for a 5-year period were calculated. This survey is a multicenter retrospective study. A questionnaire was sent to nine CI centers requesting information about patients implanted with Neurelec Digisonic(®) SP CIs. FR and CSR over a 5-year period were calculated on this group. Collaborating centers collected data on 672 patients (362 children and 310 adults) implanted between March 2006 and March 2011. The overall rate of explantation was 2.23 % (15 cases): six devices were explanted due to device failure (0.89 %) and nine were explanted for medical reasons (1.34 %). Four patients were lost to follow-up. The CSR at 5 years was 98.51 % on all patients, 98.48 % for children and 98.57 % for adults. FR was 0.97 % for adults and 0.83 % for children. This first independent study that assesses FR and CSR on the current generation of Digisonic(®) SP CI represents an important resource that can help clinicians and patients during their device choice. PMID:23161275

R?dulescu, Lumini?a; Cozma, Sebastian; Niemczyk, Casimir; Guevara, Nicolas; Gahide, Ivan; Economides, John; Lavieille, Jean-Pierre; Meller, Renaud; Bébéar, Jean-Pierre; Radafy, Emilien; Bordure, Philippe; Djennaoui, Djamel; Truy, Eric

2013-03-01

282

Polysaccharide extraction from Spirulina sp. and its antioxidant capacity.  

PubMed

To optimize polysaccharide extraction from Spirulina sp., the effect of solid-to-liquid ratio, extraction temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that extraction temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, temperature of 90°C, and time of 120 min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45 mg GAE/g sample. PMID:23541559

Chaiklahan, Ratana; Chirasuwan, Nattayaporn; Triratana, Panya; Loha, Veara; Tia, Suvit; Bunnag, Boosya

2013-07-01

283

A novel phytotoxic nonenolide from Phomopsis sp. HCCB03520.  

PubMed

A novel phytotoxic nonenolide, (6S,7R,9R)-6,7-dihydroxy-9-propylnon-4-eno-9-lactone (1), was isolated from solid cultures of the endophytic fungus Phomopsis sp. HCCB03520, together with three known compounds, cytochalasin H (2), cytochalasin N (3), and epoxycytochalasin H (4). The structures of these compounds were elucidated through spectroscopic analysis, and the absolute configurations were determined by CD spectroscopy. Phytotoxic activities of compounds 1-4 were also investigated. Compound 1 showed phytotoxic activity on germination and radicle growth of Medicago sativa, Trifolium hybridum, and Buchloe dactyloides. PMID:22344916

Yang, Zhijun; Ge, Mei; Yin, Yu; Chen, Yi; Luo, Minyu; Chen, Daijie

2012-02-01

284

[Biological activity of Penicillium sp. 10-51 exometabolites].  

PubMed

Silica gel column chromatography (silica gel "L" II kind of activity 100/160 mkm) of the chloroform extract from the cultural filtrate of Penicillium sp. 10-51 gave two fractions (chloroform and chloroform-acetone, 5:1) having biological activity. Recrystallization yielded two compounds. On the basis of physico-chemical and spectral data these compounds were identified as curvularin and hydroxycurvularin, which have a large spectrum of biological action as to bacteria, yeast, blue-green algae and phytopathogenic micromycetes. PMID:23088100

Savchuk, Ia I; Za?chenko, A M; Tsyganenko, E S

2012-01-01

285

New Sinularianin Sesquiterpenes from Soft Coral Sinularia sp.  

PubMed Central

Four new sesquiterpenes, sinularianins C–F (3–6), together with known sinularianins A (1) and B (2) were identified from a South China Sea soft coral Sinularia sp. Compounds 1–6 were evaluated for inhibition of NF-?B activation using the cell-based HEK293 NF-?B luciferase reporter gene assay. Compounds 1 and 4 were exhibited a potent effect with inhibitory rates of 41.3% and 43.0% at the concentration of 10 µg/mL, respectively. PMID:24317466

Yang, Bin; Liao, Shengrong; Lin, Xiuping; Wang, Junfeng; Liu, Juan; Zhou, Xuefeng; Yang, Xianwen; Liu, Yonghong

2013-01-01

286

Bacillus sp. BS061 Suppresses Powdery Mildew and Gray Mold  

PubMed Central

The use of a microorganism, or its secretions, to prevent plant disease offers an attractive alternative or supplement to synthetic fungicides for the management of plant disease without the negative effects of chemical control mechanisms. During a screening for microorganisms with the potential to be used as microbial fungicides, Bacillus sp. BS061 was isolated from a plant leaf. The strain BS061 potently inhibited the mycelial growth of Botrytis cinerea, and significantly reduced disease incidence of powdery mildew in cucumber and strawberry. We also found that the culture filtrate of BS061 inhibited the mycelial growth of various plant pathogens. PMID:23874134

Kim, Young-Sook; Song, Ja-Gyeong; Lee, In-Kyoung; Yeo, Woon-Hyung

2013-01-01

287

Facklamia ignava sp. nov., Isolated from Human Clinical Specimens  

PubMed Central

Two strains of a hitherto-undescribed gram-positive, catalase-negative coccus isolated from human sources were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown strains are genealogically identical and constitute a new line close to, but distinct from, Facklamia hominis. The unknown bacterium was readily distinguished from F. hominis by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Facklamia ignava sp. nov. The type strain of Facklamia ignava is CCUG 37419. PMID:9650988

Collins, Matthew D.; Lawson, Paul A.; Monasterio, Rafael; Falsen, Enevold; Sjoden, Berit; Facklam, Richard R.

1998-01-01

288

Halogenated Cyclic Peptides Isolated From the Sponge Corticium sp  

PubMed Central

Fractionation of two Fijian specimens of the sponge Corticium sp., led to the isolation of the known active alkaloid steroid plakinamine A and two new halogenated cyclic peptides, corticiamide A (1) and cyclocinamide B (2). Structural elucidation of 1 and 2 was achieved by an extensive combination of high field NMR and HRFT MS/MS experiments, and the absolute stereochemistry of 2 was determined by acid hydrolysis and Marfey’s analysis. Corticiamide A (1) and cyclocinamide B (2) represent the first peptides to be described from the genus Corticium. PMID:17391049

Laird, Damian W.; LaBarbera, Daniel V.; Feng, Xidong; Bugni, Tim S.; Harper, Mary Kay; Ireland, Chris M.

2008-01-01

289

Candida mengyuniae sp. nov., a metsulfuron-methyl-resistant yeast.  

PubMed

A metsulfuron-methyl-resistant yeast strain, JHL(T), was isolated from metsulfuron-methyl-contaminated soil collected in Jiangsu Province, China. Through morphological and physiological analysis as well as a molecular phylogenetic analysis based on the 26S rRNA gene D1/D2 region and internal transcribed spacer (ITS), this strain, which forms a clade with Candida vartiovaarae and a teleomorphic species, Williopsis saturnus, was revealed to represent a novel species in the genus Candida. The name Candida mengyuniae sp. nov. (type strain JHL(T)=CGMCC 2.3681(T)=CBS 10845(T)) is proposed for this novel species. PMID:19406825

Chen, Bo; Huang, Xing; Zheng, Jin-Wei; Li, Shun-Peng; He, Jian

2009-05-01

290

Uranium biosorption by Padina sp. algae biomass: kinetics and thermodynamics  

Microsoft Academic Search

Introduction  Kinetic, thermodynamic, and equilibrium isotherms of the biosorption of uranium ions onto Padina sp., a brown algae biomass, in a batch system have been studied.\\u000a \\u000a \\u000a \\u000a \\u000a Discussion  The kinetic data were found to follow the pseudo-second-order model. Intraparticle diffusion is not the sole rate-controlling\\u000a factor. The equilibrium experimental results were analyzed in terms of Langmuir isotherm depending with temperature. Equilibrium\\u000a data fitted

Mohammad Hassan Khani

291

Cupriavidus plantarum sp. nov., a plant-associated species.  

PubMed

During a survey of plant-associated bacteria in northeast Mexico, a group of 13 bacteria was isolated from agave, maize and sorghum plants rhizosphere. This group of strains was related to Cupriavidus respiraculi (99.4 %), but a polyphasic investigation based on DNA-DNA hybridization analysis, other genotypic studies and phenotypic features showed that this group of strains actually belongs to a new Cupriavidus species. Consequently, taking all the results together, the description of Cupriavidus plantarum sp. nov. is proposed. PMID:25098225

Estrada-de Los Santos, Paulina; Solano-Rodríguez, Roosivelt; Matsumura-Paz, Lucía Tomiko; Vásquez-Murrieta, María Soledad; Martínez-Aguilar, Lourdes

2014-11-01

292

Triterpene lactones from cultures of Ganoderma sp. KM01.  

PubMed

A revised structure of colossolactone G (1), seven new triterpene lactones, ganodermalactones A-G (2-8), and five known triterpene lactones, colossolactone I (9), schisanlactone B (10), colossolactone B (11), colossolactone E (12), and colossolactone IV (13), and ergosterol have been isolated from cultured biomass of the macrofungi Ganoderma sp. KM01. Their structures were identified by spectroscopic methods. Structures and relative configurations of 3, 7, and 8 were confirmed by X-ray crystallographic analysis. Compounds 7, 10, and 12 exhibited antimalarial activity against Plasmodium falciparum in the range 6.0-10.0 ?M (IC50). PMID:24992637

Lakornwong, Waranya; Kanokmedhakul, Kwanjai; Kanokmedhakul, Somdej; Kongsaeree, Palangpon; Prabpai, Samran; Sibounnavong, Phoutthasone; Soytong, Kasem

2014-07-25

293

High production of laccase by a new basidiomycete, Trametes sp  

Microsoft Academic Search

A new basidiomycete, Trametes sp. 420, produced laccase at 6,810 U l?1 (268 mg, 25.4 U mg?1 protein for guaiacol) in glucose medium and 7,870 U l?1 (310 mg) in cellobiose medium with induction by 0.5 mM Cu2+ and 6 mM o-toluidine. Laccase isozyme E (LacE) was the sole laccase in the fermentation products. It was stable at pH 5–9 and below\\u000a 70°C over 30 min. The K\\u000a m values of

Pingui Tong; Yuzhi Hong; Yazhong Xiao; Min Zhang; Xiaoming Tu; Tengjiao Cui

2007-01-01

294

Juanlimycins A and B, ansamycin macrodilactams from Streptomyces sp.  

PubMed

Ansamycins are a family of macrolactams characterized by an aromatic chromophore with an aliphatic chain (ansa chain) connected back to a nonadjacent position through an amide bond. This family has shown a high degree of druggability exemplified by rifamycins, maytansinoids, and geldanamycins. In this study, the isolation of two novel ansamycin macrodilactams with unprecedented features, juanlimycins A (1) and B (2), from Streptomyces sp. LC6 were reported. The structures of 1 and 2 were assigned on the basis of analysis of NMR spectroscopic data and X-ray single crystal diffraction. PMID:24797062

Zhang, Juanli; Qian, Zhengyi; Wu, Xingkang; Ding, Yanjiao; Li, Jianfang; Lu, Chunhua; Shen, Yuemao

2014-05-16

295

A new cytosporone derivative from the endophytic fungus Cytospora sp.  

PubMed

Japanese oak wilt (JOW) is a tree disease caused by the fungus Raffaelea quercivora, which is vectored by the ambrosia beetle, Platypus quercivorus. In a screening study of the inhibitory active compounds from fungi, a new cytosporone analogue, compound 1, was isolated from the endophytic fungus Cytospora sp. TT-10 isolated from Japanese oak, together with the known compounds, integracin A (2), cytosporones N (3) and A (4). Their structures were determined by extensive 1D- and 2D-NMR spectroscopic and mass spectral analyses. Compound 1 was identified as 4,5-dihydroxy-3-heptylphthalide and named cytosporone E. Compounds 2 and 3 showed antimicrobial activity against Raffaelea quercivora. PMID:25230507

Takano, Tomoya; Koseki, Takuya; Koyama, Hiromasa; Shiono, Yoshihito

2014-07-01

296

SP-100 nuclear space power systems with application to space commercialization  

NASA Technical Reports Server (NTRS)

The technology of the SP-100 space nuclear power system program is compared to that of more familiar solar-power systems. The SP-100 program develops, validates, and demonstrates the technology for space nuclear power systems in the range of 10 to 1000 kilowatts electric for use in future military and civilian space missions. Mission applications, including earth orbiting platforms and lunar/Mars surface power, are enhanced or made possible by SP-100 technology. Attention is given to the SP-100 reference flight system design, the SP-100 nuclear reactor and nuclear-reactor shield, the platform-mounted, tethered, and free-flying reactors, and installation, operation, and disposal options, as well as lunar-Mars surface applications. The SP-100 is presented as one of the nuclear energy sources needed for long-life, compact, lightweight, continuous high power independent of solar orientation, specific orbits, or missions.

Smith, J. M.

1990-01-01

297

Sewage effluent as a source of Campylobacter sp. in a surface water catchment.  

PubMed

Campylobacter sp. can regularly be found in wastewater-affected surface waters. The occurrence of Campylobacter sp. in rivers, treated sewage and combined sewer overflows was analysed in a catchment with sparse annual precipitation. During regular treatment the reduction efficacy for Campylobacter sp. varies between 1.75 and 3.5 log(10). However, Campylobacter sp. concentrations do not increase downstream in the river as more sewage treatment plants discharge into it. During rain events, the Campylobacter sp. concentration in the river upstream of any sewage plant rises and in the sewer overflow water it is more than 150-fold higher than the average concentration in the river water at the river mouth. The highest Campylobacter sp. loads and the highest risk of infection occur during summertime after heavy rainfall. Risk management strategies should focus on problems regarding water scarcity, reuse of sewage effluent and the impact of heavy rain events. PMID:20183194

Rechenburg, Andrea; Kistemann, Thomas

2009-08-01

298

Statistical optimization of culture media for production of phycobiliprotein by Synechocystis sp. PCC 6701  

Microsoft Academic Search

Synechocystis sp. PCC 6701 has a brilliantly colored pigment, phycobiliprotein containing phycoerythrin. Culture medium was optimized by\\u000a sequential designs in order to maximize phycobiliprotein production. The observed fresh weights after 6 days were 0.58 g\\/L\\u000a in BG-11, 0.83 g\\/L in medium for Scenedesmus sp. and 0.03?0.52 g\\/L in the other tested media. Medium for Scenedesmus sp. was selected to be

Seong-Joo Hong; Choul-Gyun Lee

2008-01-01

299

Effect of salt stress on the physiology of Frankia sp strain CcI6.  

PubMed

Actinorhizal plants are able to overcome saline soils and reclaim land. Frankia sp strain CcI6 was isolated from nodules of Casuarina cunninghamiana found in Egypt. Phylogenetic analysis of Frankia sp. strain CcI6 revealed that the strain is closely related to Frankia sp. strain CcI3. The strain displays an elevated level of NaCl tolerance. Vesicle production and nitrogenase activity were also influenced by NaCl. PMID:24287648

Oshone, Rediet; Mansour, Samira R; Tisa, Louis S

2013-11-01

300

Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., endemic yeasts associated with Protea flowers in South Africa.  

PubMed

In a taxonomic study of yeasts recovered from nectar of flowers and associated insects in South Africa, 11 strains were found to represent two novel species. Morphological and physiological characteristics and sequence analyses of the large-subunit rRNA gene D1/D2 region, as well as the actin, RNA polymerase II and elongation factor 2 genes, showed that the two novel species belonged to the genus Metschnikowia. Metschnikowia drakensbergensis sp. nov. (type strain EBD-CdVSA09-2(T)?=?CBS 13649(T)?=?NRRL Y-63721(T); MycoBank no. MB809688; allotype EBD-CdVSA10-2(A)?=?CBS13650(A)?=?NRRL Y-63720(A)) was recovered from nectar of Protea roupelliae and the beetle Heterochelus sp. This species belongs to the large-spored Metschnikowia clade and is closely related to Metschnikowia proteae, with which mating reactions and single-spored asci were observed. Metschnikowia caudata sp. nov. (type strain EBD-CdVSA08-1(T)?=?CBS 13651(T)?=?NRRL Y-63722(T); MycoBank no. MB809689; allotype EBD-CdVSA57-2(A)?=?CBS 13729(A)?=?NRRL Y-63723(A)) was isolated from nectar of Protea dracomontana, P. roupelliae and P. subvestita and a honeybee, and is a sister species to Candida hainanensis and Metschnikowia lopburiensis. Analyses of the four sequences demonstrated the existence of three separate phylotypes. Intraspecies matings led to the production of mature asci of unprecedented morphology, with a long, flexuous tail. A single ascospore was produced in all compatible crosses, regardless of sequence phylotype. The two species appear to be endemic to South Africa. The ecology and habitat specificity of these novel species are discussed in terms of host plant and insect host species. PMID:25106927

de Vega, Clara; Guzmán, Beatriz; Steenhuisen, Sandy-Lynn; Johnson, Steven D; Herrera, Carlos M; Lachance, Marc-André

2014-11-01

301

Reclassification of Gluconacetobacter hansenii strains and proposals of Gluconacetobacter saccharivorans sp. nov. and Gluconacetobacter nataicola sp. nov.  

PubMed

Ten strains previously assigned to Acetobacter hansenii (=Gluconacetobacter hansenii), Acetobacter pasteurianus LMG 1584 and eight reference strains of the genus Gluconacetobacter were reclassified by 16S rRNA gene sequencing, DNA-DNA similarity, DNA base composition and phenotypic characteristics. The A. hansenii strains and A. pasteurianus LMG 1584 were included in the cluster of acetic acid bacteria (family Acetobacteraceae) by 16S rRNA gene sequences. Further, they were separated into seven distinct groups by DNA-DNA similarity. DNA-DNA similarity group I was identified as G. hansenii. DNA-DNA similarity group II was retained as Gluconacetobacter sp., because DNA-DNA similarity between the strain and Gluconacetobacter entanii LTH 4560(T) could not be determined. This was due to a lack of availability of the type strain from any source. DNA-DNA similarity group III was regarded as a novel species, for which the name Gluconacetobacter saccharivorans sp. nov. (type strain, LMG 1582(T)=NRIC 0614(T)) is proposed. DNA-DNA similarity group IV included the type strains of Gluconacetobacter oboediens and Gluconacetobacter intermedius, and three A. hansenii strains. This group was identified as G. oboediens because high values of DNA-DNA similarity were obtained between the type strains and G. oboediens has priority over G. intermedius. DNA-DNA similarity group V was identified as Gluconacetobacter europaeus. DNA-DNA similarity group VI was regarded as a novel species, for which the name Gluconacetobacter nataicola sp. nov. (type strain, LMG 1536(T)=NRIC 0616(T)) is proposed. DNA-DNA similarity group VII was reclassified as Gluconacetobacter xylinus. The description of G. hansenii is emended. PMID:16957106

Lisdiyanti, Puspita; Navarro, Richard R; Uchimura, Tai; Komagata, Kazuo

2006-09-01

302

Migration, site selection, and development of Ornithodiplostomum sp. metacercariae (Digenea: Strigeoidea) in fathead minnows (Pimephales promelas)  

E-print Network

: Strigeoidea) in fathead minnows (Pimephales promelas) Chelsea E. Matisz *, Cameron P. Goater Department of Ornithodiplostomum sp. metacercariae in their second intermediate host, the fathead minnow, Pimephales promelas

Wisenden, Brian D.

303

Structure of SP-B/DPPC Mixed Films Studied by Neutron Reflectometry  

PubMed Central

The structures of films of pulmonary surfactant protein B (SP-B) and mixtures of SP-B and dipalmitoylphosphatidylcholine (DPPC) at the air/water interface have been studied by neutron reflectometry and Langmuir film balance methods. From the film balance studies, we observe that the isotherms of pure DPPC and SP-B/DPPC mixtures very nearly overlay one another at very high pressures, suggesting that the SP-B is being excluded from the film. The use of multiple contrasts with neutron reflectometry at a range of surface pressures has enabled the mixing and squeeze out of the DPPC and SP-B mixtures to be studied. We can identify the SP-B component of the interfacial structure and its position as a function of surface pressure. The mixtures are initially a homogeneous layer at low surface pressures. At higher surface pressures, the SP-B is squeezed out of the lipid layer into the subphase, with the first signs detected at 30 mN m?1. At 50 mN m?1, the subphase is almost completely excluded from the DPPC layer, with the SP-B content significantly reduced. Only a small amount of DPPC appears to be associated with the squeezed out SP-B. PMID:18708453

Fullagar, W. K.; Holt, S. A.; Gentle, I. R.

2008-01-01

304

Sp8 and COUP-TF1 reciprocally regulate patterning and Fgf signaling in cortical progenitors.  

PubMed

To gain new insights into the transcriptional regulation of cortical development, we examined the role of the transcription factor Sp8, which is downstream of Fgf8 signaling and known to promote rostral cortical development. We have used a binary transgenic system to express Sp8 throughout the mouse telencephalon in a temporally restricted manner. Our results show that misexpression of Sp8 throughout the telencephalon, at early but not late embryonic stages, results in cortical hypoplasia, which is accompanied by increased cell death, reduced proliferation, and precocious neuronal differentiation. Misexpression of Sp8 at early developmental stages represses COUP-TF1 expression, a negative effector of Fgf signaling and a key promoter of posterior cortical identity, while ablation of Sp8 has the opposite effect. In addition, transgenic misexpression of COUP-TF1 resulted in downregulation of Sp8, indicating a reciprocal cross-regulation between these 2 transcription factors. Although Sp8 has been suggested to induce and/or maintain Fgf8 expression in the embryonic telencephalon, neither Fgf8 nor Fgf15 was upregulated using our gain-of-function approach. However, misexpression of Sp8 greatly increased the expression of Fgf target molecules, suggesting enhanced Fgf signaling. Thus, we propose that Sp8 promotes rostral and dorsomedial cortical development by repressing COUP-TF1 and promoting Fgf signaling in pallial progenitors. PMID:23307639

Borello, Ugo; Madhavan, Mayur; Vilinsky, Ilya; Faedo, Andrea; Pierani, Alessandra; Rubenstein, John; Campbell, Kenneth

2014-06-01

305

Isolation and process parameter optimization of Aspergillus sp. for removal of chromium from tannery effluent.  

PubMed

Five morphologically different fungi were isolated from leather tanning effluent in which Aspergillus sp. and Hirsutella sp. had higher potential to remove chromium. The potential of Aspergillus sp. for removal of chromium was evaluated in shake flask culture in different pH, temperature, inoculums size, carbon and nitrogen source. The maximum chromium was removed at pH 6, temperature 30 degrees C, sodium acetate (0.2%) and yeast extract (0.1%). Aspergillus sp. was applied in 2l bioreactor for removal of chromium, and it was observed that 70% chromium was removed after 3 days. PMID:16023341

Srivastava, Shaili; Thakur, Indu Shekhar

2006-07-01

306

Occurrence of a Thiothrix sp. Attached to Mayfly Larvae and Presence of Parasitic Bacteria in the Thiothrix sp  

PubMed Central

Larvae of the mayfly (Drunella grandis [Eaton]) from Diamond Fork Creek, Utah, were covered with a heavy growth of the sulfide-oxidizing bacterium Thiothrix. The bacterium did not seem to harm the mayfly, but the Thiothrix trichomes were parasitized by three morphologically distinct bacteria, two of which were cytoplasmic and one of which was probably periplasmic. At least two of the parasites destroyed the cytoplasmic contents of the Thiothrix sp., thus killing the host cell. Attempts to obtain the parasites in pure culture were unsuccessful. Images PMID:16348112

Larkin, John M.; Henk, Margaret C.; Burton, Sheril D.

1990-01-01

307

Occurrence of a Thiothrix sp. Attached to Mayfly Larvae and Presence of Parasitic Bacteria in the Thiothrix sp.  

PubMed

Larvae of the mayfly (Drunella grandis [Eaton]) from Diamond Fork Creek, Utah, were covered with a heavy growth of the sulfide-oxidizing bacterium Thiothrix. The bacterium did not seem to harm the mayfly, but the Thiothrix trichomes were parasitized by three morphologically distinct bacteria, two of which were cytoplasmic and one of which was probably periplasmic. At least two of the parasites destroyed the cytoplasmic contents of the Thiothrix sp., thus killing the host cell. Attempts to obtain the parasites in pure culture were unsuccessful. PMID:16348112

Larkin, J M; Henk, M C; Burton, S D

1990-02-01

308

Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov., leaf-associated bacteria isolated from Jatropha curcas L.  

PubMed

Four orange-pigmented isolates, L7-456, L7-484(T), L9-479 and L9-753(T), originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all four isolates belong to the genus Aureimonas. In these analyses, strain L7-484(T) appeared to be most closely related to Aureimonas ureilytica 5715S-12(T) (95.7?% sequence identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753(T) were found to be identical and also shared the highest similarity with A. ureilytica 5715S-12(T) (97.5?%). Both L7-484(T) and L9-753(T) contained Q-10 and Q-9 as predominant ubiquinones and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sulfoquinovosyldiacylglycerol and an aminophospholipid as the major polar lipids. C18?:?1?7c and C16?:?0 were the major fatty acids. Similar to other species in the genus Aureimonas, hydroxylated fatty acids (e.g. C18?:?1 2-OH) and cyclic fatty acids (C19?:?0 cyclo ?8c) were also present. The DNA G+C contents of L7-484(T) and L9-753(T) were 66.1 and 69.4 mol%, respectively. Strains L7-484(T) and L9-753(T) exhibited less than 40?% DNA-DNA hybridization both between themselves and to A. ureilytica KACC 11607(T). Our results support the proposal that strain L7-484(T) represents a novel species within the genus Aureimonas, for which the name Aureimonas jatrophae sp. nov. is proposed, and that strains L9-753(T), L7-456 (?=?KACC 16229 ?=?DSM 25023) and L9-479 (?=?KACC 16228 ?=?DSM 25024) represent a second novel species within the genus, for which the name Aureimonas phyllosphaerae sp. nov. is proposed. The type strains of Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov. are respectively L7-484(T) (?=?KACC 16230(T) ?=?DSM 25025(T)) and L9-753(T) (?=?KACC 16231(T) ?=?DSM 25026(T)). PMID:22922534

Madhaiyan, M; Hu, C J; Jegan Roy, J; Kim, S-J; Weon, H-Y; Kwon, S-W; Ji, L

2013-05-01

309

Babesial Vector Tick Defensin against Babesia sp. Parasites? †  

PubMed Central

Antimicrobial peptides are major components of host innate immunity, a well-conserved, evolutionarily ancient defensive mechanism. Infectious disease-bearing vector ticks are thought to possess specific defense molecules against the transmitted pathogens that have been acquired during their evolution. We found in the tick Haemaphysalis longicornis a novel parasiticidal peptide named longicin that may have evolved from a common ancestral peptide resembling spider and scorpion toxins. H. longicornis is the primary vector for Babesia sp. parasites in Japan. Longicin also displayed bactericidal and fungicidal properties that resemble those of defensin homologues from invertebrates and vertebrates. Longicin showed a remarkable ability to inhibit the proliferation of merozoites, an erythrocyte blood stage of equine Babesia equi, by killing the parasites. Longicin was localized at the surface of the Babesia sp. parasites, as demonstrated by confocal microscopic analysis. In an in vivo experiment, longicin induced significant reduction of parasitemia in animals infected with the zoonotic and murine B. microti. Moreover, RNA interference data demonstrated that endogenous longicin is able to directly kill the canine B. gibsoni, thus indicating that it may play a role in regulating the vectorial capacity in the vector tick H. longicornis. Theoretically, longicin may serve as a model for the development of chemotherapeutic compounds against tick-borne disease organisms. PMID:17485458

Tsuji, Naotoshi; Battsetseg, Badgar; Boldbaatar, Damdinsuren; Miyoshi, Takeharu; Xuan, Xuenan; Oliver, James H.; Fujisaki, Kozo

2007-01-01

310

Metabolic engineering of indene bioconversion in Rhodococcus sp.  

PubMed

We have applied the methodology of metabolic engineering in the investigation of the enzymatic bioreaction network in Rhodococcus sp. that catalyzes the bioconversion of indene to (2R)-indandiol suitable for the synthesis of cis-1-amino-2-indanol, a precursor of the HIV protease inhibitor, Crixivan. A chemostat with a novel indene air delivery system was developed to facilitate the study of steady state physiology of Rhodococcus sp. 124. Prolonged cultivation of this organism in a continuous flow system led to the evolution of a mutant strain, designated KY1, with improved bioconversion properties, in particular a twofold increase in yield of (2R)-indandiol relative to 124. Induction studies with both strains indicated that KY1 lacked a toluene-inducible dioxygenase activity present in 124 and responsible for the formation of undesired byproducts. Flux analysis of indene bioconversion in KY1 performed using steady state metabolite balancing and labeling with [14C]-tracers revealed that at least 94% of the indene is oxidized by a monooxygenase to indan oxide that is subsequently hydrolyzed to trans-(1R,2R)-indandiol and cis-(1S,2R)-indandiol. This analysis identified several targets in KY1 for increasing (2R)-indandiol product yield. Most promising among them is the selective hydrolysis of indan oxide to trans-(1R,2R)-indandiol through expression of an epoxide hydrolase or modification of culture conditions. PMID:11816813

Stafford, D E; Yanagimachi, K S; Stephanopoulos, G

2001-01-01

311

SP-100 power system conceptual design for lunar base applications  

NASA Technical Reports Server (NTRS)

A conceptual design is presented for a nuclear power system utilizing an SP-100 reactor and multiple Stirling cycle engines for operation on the lunar surface. Based on the results of this study, it was concluded that this power plant could be a viable option for an evolutionary lunar base. The design concept consists of a 2500 kWt (kilowatt thermal) SP-100 reactor coupled to eight free-piston Stirling engines. Two of the engines are held in reserve to provide conversion system redundancy. The remaining engines operate at 91.7 percent of their rated capacity of 150 kWe. The design power level for this system is 825 kWe. Each engine has a pumped heat-rejection loop connected to a heat pipe radiator. Power system performance, sizing, layout configurations, shielding options, and transmission line characteristics are described. System components and integration options are compared for safety, high performance, low mass, and ease of assembly. The power plant was integrated with a proposed human lunar base concept to ensure mission compatibility. This study should be considered a preliminary investigation; further studies are planned to investigate the effect of different technologies on this baseline design.

Mason, Lee S.; Bloomfield, Harvey S.; Hainley, Donald C.

1989-01-01

312

Biosorption of phenol onto bionanoparticles from Spirulina sp. LEB 18.  

PubMed

The biosorption of phenol onto bionanoparticles from Spirulina sp. LEB 18 was studied. Firstly, the bionanoparticles were prepared from Spirulina sp. strain LEB 18 and characterized. After, response surface methodology was employed to optimize the biosorption process as a function of pH (3.2-8.8) and bionanoparticles dosage (0.15-1.85 g L(-1)). Finally, equilibrium and thermodynamic studies were performed at different temperatures (298-328 K). The bionanoparticles presented hydrodynamic diameter of 232±3 nm and polydispersity index of 0.150. It was found that the more adequate condition for the phenol biosorption was pH of 6.0 and bionanoparticles dosage of 1.85 g L(-1). The Langmuir model presented satisfactory fit with the equilibrium experimental data. The maximum biosorption capacity was 159.33 mg g(-1), obtained at 298 K. The thermodynamic parameters showed that the biosorption was a spontaneous, favorable and exothermic process. Based on these results, it can be affirmed that the bionanoparticles are an alternative, renewable and eco-friendly biosorbent to removal phenol from aqueous solutions. PMID:23849821

Dotto, G L; Gonçalves, J O; Cadaval, T R S; Pinto, L A A

2013-10-01

313

SP-100 operational life model. Fiscal Year 1990 annual report  

SciTech Connect

This report covers the initial year`s effort in the development of an Operational Life Model (OLM) for the SP-100 Space Reactor Power System. The initial step undertaken in developing the OLM was to review all available documentation from GE on their plans for the OLM and on the degradation and failure mechanisms envisioned for the SP-100. In addition, the DEGRA code developed at JPL, which modelled the degradation of the General Purpose Heat Source based Radioisotope Thermoelectric Generator (GPHS-RTG), was reviewed. Based on the review of the degradation and failure mechanisms, a list of the most pertinent degradation effects along with their key degradation mechanisms was compiled. This was done as a way of separating the mechanisms from the effects and allowing all of the effects to be incorporated into the OLM. The emphasis was on parameters which will tend to change performance as a function of time and not on those that are simply failures without any prior degradation.

Ewell, R.; Awaya, H.

1990-12-14

314

Zygosaccharomyces sapae sp. nov., isolated from Italian traditional balsamic vinegar.  

PubMed

Fourteen yeast isolates were recovered from two traditional balsamic vinegar (TBV) samples collected in the provinces of Modena and Reggio Emilia, Italy. Microsatellite-primed-PCR (MSP-PCR) was used to de-replicate the isolate collection into two representative strains, ABT301(T) and ABT601. Phylogenetic analysis based on the D1/D2 domains of the 26S rRNA gene indicated that these strains represented a distinct species of the genus Zygosaccharomyces, closely related to Zygosaccharomyces rouxii and Zygosaccharomyces mellis. Physiological and morphological tests supported the recognition of a novel taxon of halotolerant, osmotolerant, non-psychrotolerant and maltose-fermentation-negative yeasts showing a chain or star-shaped pattern of budding cells, which remained attached to each other. Morphological observations offered evidence of ascospore formation. A novel species, Zygosaccharomyces sapae sp. nov., is proposed to accommodate these strains, with strain ABT301(T) (= CBS 12607(T) = MUCL 54092(T)) as the type strain. Based on D1/D2 domain phylogenetic analysis, the novel strains shared the highest sequence similarity (100 %) with Zygosaccharomyces sp. strain NCYC 3042, previously isolated from sugar [James, S. A., Bond, C. J., Stratford, M. & Roberts, I. N. (2005). FEMS Yeast Res 5, 747-755]. However, based on phylogenetic (internal transcribed spacers, ITS), PCR fingerprinting and physiological analyses, marked differences were observed between the novel species and strain NCYC 3042, and these results are discussed in more detail. PMID:23024146

Solieri, Lisa; Chand Dakal, Tikam; Giudici, Paolo

2013-01-01

315

Transesterification of triglycerides by dried biomass of Aspergillus sp.  

PubMed

Fungus isolate, Aspergillus sp. (RBD01), which was isolated from biocontaminated clarified butter was evaluated for its potential to transesterify used edible and non-edible oils for generation of alkyl esters, when used as biocatalyst as dry biomass. The work aimed at determining the potential of dry biomass of Aspergillus sp. (RBD01) to transesterify used cottonseed oil and non-edible oils viz., jatropha and karanj under various culture conditions. A conversion of oil (cotton seed) to ethyl ester to the extent of 84% was obtained at reaction temperature of 35°C, with 20% biomass and step-wise addition of ethanol at 1:5 molar ratio (oil to ethanol), within total reaction time of 36 h. Under similar conditions, transesterification of Jatropha and Karanj oils resulted in only 75 and 78.2% ethyl ester. Further, with reference to the effect of frying on transesterification, increase in frying time decreased the extent of transesterification from 84% to 30%. PMID:23648404

Aulakh, Satnam Singh; Prakash, N Tejo; Prakash, Ranjana

2013-01-01

316

Mechanisms of coactivation of estrogen receptor alpha (ER alpha)- and ER alpha/Sp-mediated gene transactivation by vitamin D receptor interacting protein 205 (DRIP205) in breast cancer cells  

E-print Network

coactivate ER? and ER?/Sp1. RNA interference study showed that DRIP205 coactivation of ER?/Sp was abolished in cells transfected with iSp3 and iSp4, suggesting that Sp3 and Sp4 are required for coactivation of ER?/Sp by DRIP205 in ZR-75 cells....

Wu, Qian

2009-05-15

317

Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya.  

PubMed

SDS-PAGE of total bacterial proteins was applied to the classification of 25 Sudanese and five Kenyan strains isolated from the root nodules of Acacia senegal and Prosopis chilensis. Twenty strains were also studied by multilocus enzyme electrophoresis (MLEE) and the whole 16S rRNA gene was sequenced from two strains representing the two major clusters. These results, together with the previously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, DNA-DNA dot-blot hybridization, genomic fingerprinting using repetitive sequence-based PCR, DNA base composition analysis, DNA-DNA reassociation analysis, partial sequencing of the 16S rRNA gene and RFLP analysis of the amplified 16S rRNA gene, showed that all 30 strains belong to the genus Sinorhizobium. Two of the strains grouped with Sinorhizobium saheli and seven with Sinorhizobium terangae, while the rest did not cluster with any of the established species. The majority of the strains formed two phenotypically and genotypically distinct groups and we therefore propose that these strains should be classified as two new species, Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov. PMID:10555313

Nick, G; de Lajudie, P; Eardly, B D; Suomalainen, S; Paulin, L; Zhang, X; Gillis, M; Lindström, K

1999-10-01

318

Ctenascarophis lesteri n. sp. and Prospinitectus exiguus n. sp. (Nematoda: Cystidicolidae) from the skipjack tuna, Katsuwonus pelamis.  

PubMed

Two cystidicolid nematodes, Ctenascarophis lesteri n. sp. and Prospinitectus exiguus n. sp., are described from the skipjack tuna, Katsuwonus pelamis, from Fiji, New Caledonia, Marquesas Islands, New Zealand, Papua New Guinea, and Puerto Rico. The former species differs from Ctenascarophis gastricus, the only other member of the genus, by having comb rows extending farther posterior than two-thirds the body length, to the anus in the female; it also has differently distributed spines on each comb, with a maximum of 32 rather than 8. The male has 7 rows of precloacal longitudinal crests; 3 pairs of precloacal, 1 pair adanal, and 6 pairs postcloacal papillae; and a spicule ratio of 1:3.4-1:5.0. The latter species differs from Prospinitectus mollis, the only other species in the genus, by being less than one-half as long, 3.5-5.9 mm long; with a proportionally longer esophagus; fewer spines per spine ring, a maximum of 49 compared with 100; deirids that lack spinules; males with an unornamented precloacal cuticle as well as a different number and distribution of caudal papillae, 2 precloacal and 7 postcloacal; and a smaller spicule ratio, 1:4 rather than 1:5-1:7. PMID:8277376

Crites, J L; Overstreet, R M; Maung, M

1993-12-01

319

Reconstruction and Comparison of the Metabolic Potential of Cyanobacteria Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803  

PubMed Central

Cyanobacteria are an important group of photoautotrophic organisms that can synthesize valuable bio-products by harnessing solar energy. They are endowed with high photosynthetic efficiencies and diverse metabolic capabilities that confer the ability to convert solar energy into a variety of biofuels and their precursors. However, less well studied are the similarities and differences in metabolism of different species of cyanobacteria as they pertain to their suitability as microbial production chassis. Here we assemble, update and compare genome-scale models (iCyt773 and iSyn731) for two phylogenetically related cyanobacterial species, namely Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803. All reactions are elementally and charge balanced and localized into four different intracellular compartments (i.e., periplasm, cytosol, carboxysome and thylakoid lumen) and biomass descriptions are derived based on experimental measurements. Newly added reactions absent in earlier models (266 and 322, respectively) span most metabolic pathways with an emphasis on lipid biosynthesis. All thermodynamically infeasible loops are identified and eliminated from both models. Comparisons of model predictions against gene essentiality data reveal a specificity of 0.94 (94/100) and a sensitivity of 1 (19/19) for the Synechocystis iSyn731 model. The diurnal rhythm of Cyanothece 51142 metabolism is modeled by constructing separate (light/dark) biomass equations and introducing regulatory restrictions over light and dark phases. Specific metabolic pathway differences between the two cyanobacteria alluding to different bio-production potentials are reflected in both models. PMID:23133581

Saha, Rajib; Verseput, Alex T.; Berla, Bertram M.; Mueller, Thomas J.; Pakrasi, Himadri B.; Maranas, Costas D.

2012-01-01

320

Geotrichum siamensis sp. nov. and Geotrichum phurueaensis sp. nov., two asexual arthroconidial yeast species isolated in Thailand.  

PubMed

Two asexual arthroconidial yeast strains, TM3-44(T) and LYSM5(T), were isolated, respectively, from estuarine water in a mangrove forest and soil in a terrestrial forest in Thailand. Analysis of the D1/D2 domains of the large-subunit rRNA gene sequences revealed that strain TM3-44(T) differed from the closest species in terms of pairwise sequence similarity, Dipodascus albidus, by 11.7% nucleotide substitutions, while strain LYSM5(T) was closest to Galactomyces geotrichum with only 2.9% nucleotide substitutions. The phylogenetic tree further demonstrated that strain TM3-44(T) was at a distant position from the closest species, D. albidus, and other related species in the Dipodascus clade, while strain LYSM5(T) clustered with G. geotrichum, it closest relative in the Galactomyces clade. The phenotypic characteristics of the two strains were typical of the genus Geotrichum. On the basis of the above findings, strain TM3-44(T) was assigned as a novel species of Geotrichum, for which the name Geotrichum siamensis sp. nov. is proposed. The type strain is TM3-44(T) (BCC 29903(T)=NBRC 104880(T)=CBS 10929(T)). Strain LYSM5(T) represented another novel species of Geotrichum, which was named Geotrichum phurueaensis sp. nov. The type strain is LYSM5(T) (BCC 34756(T)=NBRC 105674(T)=CBS 11418(T)). PMID:19788556

Kaewwichian, Rungluk; Yongmanitchai, Wichien; Srisuk, Nantana; Fujiyama, Kazuhito; Limtong, Savitree

2010-03-01

321

Three new asexual arthroconidial yeasts, Geotrichum carabidarum sp. nov., Geotrichum histeridarum sp. nov., and Geotrichum cucujoidarum sp. nov., isolated from the gut of insects.  

PubMed

Twenty arthroconidial yeasts were isolated from the digestive tract of basidiome-feeding beetles and lepidopteran larvae. All of the yeasts reproduced only asexually by arthroconidia and some by endo- or blastoconidia as well. Based on the comparisons of sequences in ribosomal RNA genes and other taxonomic characteristics, the yeasts were identified as three unknown Geotrichum species. The three new species are described as Geotrichum carabidarum (NRRL Y-27727T), G. histeridarum (NRRL Y-27729T), and G. cucujoidarum (NRRL Y-27731T). Phylogenetic analyses from ribosomal DNA sequences showed that members of the genus Geotrichum and related arthroconidial yeast taxa were divided into two major clades: (1) Dipodascus and Galactomyces with Geotrichum anamorphs including all the new species; and (2) Magnusiomyces with Saprochaete anamorphs. G. cucujoidarum formed a subclade with G. fermentans and Geotrichum sp. Y-5419, while the two closely related species, G. carabidarum and G. histeridarum, represent a new basal subclade in the clade of Geotrichum and its teleomorphs. PMID:16406553

Suh, Sung-Oui; Blackwell, Meredith

2006-02-01

322

Wood-inhabiting freshwater fungi from Thailand: Ascothailandia grenadoidia gen. et sp. nov., Canalisporium grenadoidia sp. nov. with a key to Canalisporium species (Sordariomycetes, Ascomycota)  

Microsoft Academic Search

Ascothailandia grenadoidia gen. et sp. nov. is described and illustrated from submerged wood (Wrightia tomentosa) in a stream at Hala Bala Wildlife Sanctuary, southern Thailand. The new genus (teleomorph) is characterized by perithecoid,\\u000a globose, dark brown, ostiolate ascomata, paraphysate, asci cylindrical, unitunicate with a prominent J-refractive apical ring\\u000a and versicolurus, 3-euseptate ascospores. Ascospores germinated producing a Canalisporium (C. grenadoidia sp.

Veera Sri-indrasutdhi; Nattawut Boonyuen; Satinee Suetrong; Charuwan Chuaseeharonnachai; Somsak Sivichai; E. B. Gareth Jones

2010-01-01

323

The cloned 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene from Sinorhizobium sp. strain BL3 in Rhizobium sp. strain TAL1145 promotes nodulation and growth of Leucaena leucocephala  

Microsoft Academic Search

The objective of this study was to determine the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase of symbionts in nodulation and growth of Leucaena leucocephala. The acdS genes encoding ACC deaminase were cloned from Rhizobium sp. strain TAL1145 and Sinorhizobium sp. BL3 in multicopy plasmids, and transferred to TAL1145. The BL3-acdS gene greatly enhanced ACC deaminase activity in TAL1145 compared to the

Panlada Tittabutr; Jonathan D. Awaya; Qing X. Li; Dulal Borthakur

2008-01-01

324

Linocarpon angustatum sp. nov., and Neolinocarpon nypicola sp. nov. from petioles of Nypa fruticans , and a list of fungi from aerial parts of this host  

Microsoft Academic Search

Two new ascomycetes,Linocarpon angustatum sp. nov. andNeolinocarpon nypicola sp. nov., are described from petioles ofNypa fruticans in Malaysia.Linocarpon angustatum differs from species in the genus in having needle-shaped ascospores.Neolinocarpon nypicola differs from species in the genus in having filiform ascospores which gradually taper towards the ends, and ascomata developing\\u000a within well developed stromata. These new species are compared with existing

Kevin D. Hyde; Siti A. Alias

1999-01-01

325

Biosorption of lead(II) from aqueous solutions by non-living algal biomass Oedogonium sp. and Nostoc sp.--a comparative study.  

PubMed

Industrial wastewaters containing heavy metals pose a major environmental problem that needs to be remedied. The present study reports the ability of two non-living (dried) fresh water algae, Oedogonium sp. and Nostoc sp. to remove lead(II) from aqueous solutions in batch system under varying range of pH (2.99-7.04), contact time (5-300 min), biosorbent dose (0.1-0.8 g/L), and initial metal ion concentrations (100 and 200mg/L). The optimum conditions for lead biosorption are almost same for the two algal biomass Oedogonium sp. and Nostoc sp. (pH 5.0, contact time 90 and 70 min, biosorbent dose 0.5 g/L and initial Pb(II) concentration 200mg/L) however, the biomass of Oedogonium sp. was found to be more suitable than Nostoc sp. for the development of an efficient biosorbent for the removal of lead(II) from aqueous solutions, as it showed higher values of q(e) adsorption capacity (145.0mg/g for Oedogonium sp. and 93.5mg/g for Nostoc sp.). The equilibrium data fitted well in the Langmuir isotherms than the Freundlich isotherm, thus proving monolayer adsorption of lead on both the algal biomass. Analysis of data shows that the process involves second-order kinetics and thermodynamic treatment of equilibrium data shows endothermic nature of the adsorption process. The spectrum of FTIR confirms that the amino and carboxyl groups on the surface of algal biomass were the main adsorption sites for lead removal. Both the biosorbents could be regenerated using 0.1 mol/L HCl solution, with upto 90% recovery. The biosorbents were reused in five biosorption-desorption cycles without a significant loss in biosorption capacity. Thus, this study demonstrated that both the algal biomass could be used as an efficient biosorbents for the treatment of lead(II) bearing wastewater streams. PMID:18321684

Gupta, Vinod K; Rastogi, Arshi

2008-07-15

326

Revision of the genus Pteroplistes in India, with the description of two new species Pteroplistes kervasae Jaiswara, n. sp. and Pteroplistes masinagudi Jaiswara, n. sp. (Orthoptera, Grylloidea, Pteroplistinae).  

PubMed

Pteroplistes Brunner von Wattenwyl, 1873 is an Indo-Malaysian cricket genus with only one species, P. platycleis Bolivar, 1899(1900) known from India. Here, we redescribe the genus Pteroplistes and P. platycleis and describe two new Indian species, Pteroplistes kervasae Jaiswara, n. sp. and Pteroplistes masinagudi Jaiswara, n. sp. from the Western Ghats, using morphology and genitalia. Identification keys to separate the Indian species of Pteroplistes are provided and the distribution of the genus in India is discussed. PMID:24943415

Jaiswara, Ranjana; Desutter-Grandcolas, Laure

2014-01-01

327

Isolable rotamers of N-(9-fluorenyl)-pyridinium salts owing to restricted rotation around the C(sp 3) ? N(sp 2) single bond  

NASA Astrophysics Data System (ADS)

Reaction of 9-fluorenylamine with substituted pyrylium perchlorates yields new N-(9-fluorenyl)-pyridinium perchlorates 4-6 whose 1H-NMR spectra indicate a fixed conformation around the C(sp 3) ? N(sp 2) single bond. Asymmetrically substituted systems 5, 6 can be separated into stable rotamers. Their interconversion above 120° occurs simultaneously with cleavage of the C ? N bond.

Balaban, A. T.; Gheorghiu, N. D.; Balaban, T. S.

1984-03-01

328

Induction of nitrate-dependent Fe(II) oxidation by Fe(II) in Dechloromonas sp. strain UWNR4 and Acidovorax sp. strain 2AN.  

PubMed

We evaluated the inducibility of nitrate-dependent Fe(II)-EDTA oxidation (NDFO) in non-growth, chloramphenicol-amended, resting-cell suspensions of Dechloromonas sp. strain UWNR4 and Acidovorax sp. strain 2AN. Cells previously incubated with Fe(II)-EDTA oxidized ca. 6-fold more Fe(II)-EDTA than cells previously incubated with Fe(III)-EDTA. This is the first report of induction of NDFO by Fe(II). PMID:23144134

Chakraborty, Anirban; Picardal, Flynn

2013-01-01

329

Induction of Nitrate-Dependent Fe(II) Oxidation by Fe(II) in Dechloromonas sp. Strain UWNR4 and Acidovorax sp. Strain 2AN  

PubMed Central

We evaluated the inducibility of nitrate-dependent Fe(II)-EDTA oxidation (NDFO) in non-growth, chloramphenicol-amended, resting-cell suspensions of Dechloromonas sp. strain UWNR4 and Acidovorax sp. strain 2AN. Cells previously incubated with Fe(II)-EDTA oxidized ca. 6-fold more Fe(II)-EDTA than cells previously incubated with Fe(III)-EDTA. This is the first report of induction of NDFO by Fe(II). PMID:23144134

Chakraborty, Anirban

2013-01-01

330

Oxidative coupling of alkenes with amides using peroxides: selective amide C(sp3)-H versus C(sp2)-H functionalization.  

PubMed

A new oxidative coupling of unactivated terminal alkenes with amides using peroxides is described, in which mono- and difunctionalization of alkenes are selectively achieved. In this reaction with amides, the chemoselectivity toward the functionalization of the C(sp(3))-H bonds adjacent to the nitrogen atom or the functionalization of the carbonyl C(sp(2))-H bonds across alkenes relies on the reaction conditions. PMID:25212419

Yang, Xu-Heng; Wei, Wen-Ting; Li, Hai-Bing; Song, Ren-Jie; Li, Jin-Heng

2014-11-01

331

Nicotine degradation by two novel bacterial isolates of Acinetobacter sp. TW and Sphingomonas sp. TY and their responses in the presence of neonicotinoid insecticides  

Microsoft Academic Search

Two novel nicotine-degrading bacterial strains were isolated from tobacco waste and identified as Acinetobacter sp. TW and Sphingomonas sp. TY based on morphology, physiological and biochemical tests, Biolog analysis and 16S rDNA sequencing. The 16S rDNA sequences\\u000a have been deposited in GenBank under the accession numbers FJ753401 for TW and FJ754274 for TY. The best culture conditions\\u000a for nicotine degradation

Meizhen WangGuiqin; Guiqin Yang; Xin Wang; Yanlai Yao; Hang Min; Zhenmei Lu

2011-01-01

332

Proteomic analysis of SP600125-controlled TrkA-dependent targets in SK-N-MC neuroblastoma cells: inhibition of TrkA activity by SP600125.  

PubMed

The c-Jun N-terminal kinase (JNK) is well known to play an important role in cell death signaling of the p75 neurotrophin receptor. However, little has been studied about a role of JNK in the signaling pathways of the tropomyosin-related kinase A (TrkA) neurotrophin receptor. In this study, we investigated JNK inhibitor SP600125-controlled TrkA-dependent targets by proteomic analysis to better understand an involvement of JNK in TrkA-mediated signaling pathways. PDQuest image analysis and protein identification results showed that hnRNP C1/C2, ?-tubulin, ?-tubulin homolog, actin homolog, and eIF-5A-1 protein spots were upregulated by ectopic expression of TrkA, whereas ?-enolase, peroxiredoxin-6, PROS-27, HSP70, PP1-gamma, and PDH E1-alpha were downregulated by TrkA, and these TrkA-dependent upregulation and downregulation were significantly suppressed by SP600125. Notably, TrkA largely affected certain PTM(s) but not total protein amounts of the SP600125-controlled TrkA-dependent targets. Moreover, SP600125 strongly suppressed TrkA-mediated tyrosine phosphorylation signaling pathways as well as JNK signaling, indicating that SP600125 could function as a TrkA inhibitor. Taken together, our results suggest that TrkA could play an important role in the cytoskeleton, cell death, cellular processing, and glucose metabolism through activation or inactivation of the SP600125-controlled TrkA-dependent targets. PMID:24375967

Jung, Eun Joo; Park, Hyung Chul; Chung, Ky Hyun; Kim, Choong Won

2014-02-01

333

Unusual surface and edge morphologies, sp2 to sp3 hybridized transformation and electronic damage after Ar+ ion irradiation of few-layer graphene surfaces  

PubMed Central

Roughness and defects induced on few-layer graphene (FLG) irradiated by Ar+ ions at different energies were investigated using X-ray photoemission spectroscopy (XPS) and atomic force microscopy techniques. The results provide direct experimental evidence of ripple formation, sp2 to sp3 hybridized carbon transformation, electronic damage, Ar+ implantation, unusual defects and edge reconstructions in FLG, which depend on the irradiation energy. In addition, shadowing effects similar to those found in oblique-angle growth of thin films were seen. Reliable quantification of the transition from the sp2-bonding to sp3-hybridized state as a result of Ar+ ion irradiation is achieved from the deconvolution of the XPS C (1s) peak. Although the ion irradiation effect is demonstrated through the shape of the derivative of the Auger transition C KVV spectra, we show that the D parameter values obtained from these spectra which are normally used in the literature fail to account for the sp2 to sp3 hybridization transition. In contrast to what is known, it is revealed that using ion irradiation at large FLG sample tilt angles can lead to edge reconstructions. Furthermore, FLG irradiation by low energy of 0.25 keV can be a plausible way of peeling graphene layers without the need of Joule heating reported previously. PMID:22901368

2012-01-01

334

Ethyl tert-butyl ether (ETBE) biodegradation by a syntrophic association of Rhodococcus sp. IFP 2042 and Bradyrhizobium sp. IFP 2049 isolated from a polluted aquifer.  

PubMed

Ethyl tert-butyl ether (ETBE) enrichment was obtained by adding contaminated groundwater to a mineral medium containing ETBE as the sole carbon and energy source. ETBE was completely degraded to biomass and CO2 with a transient production of tert-butanol (TBA) and a final biomass yield of 0.37 ± 0.08 mg biomass (dry weight).mg(-1) ETBE. Two bacterial strains, IFP 2042 and IFP 2049, were isolated from the enrichment, and their 16S rRNA genes (rrs) were similar to Rhodococcus sp. (99 % similarity to Rhodococcus erythropolis) and Bradyrhizobium sp. (99 % similarity to Bradyrhizobium japonicum), respectively. Rhodococcus sp. IFP 2042 degraded ETBE to TBA, and Bradyrhizobium sp. IFP 2049 degraded TBA to biomass and CO2. A mixed culture of IFP 2042 and IFP 2049 degraded ETBE to CO2 with a biomass yield similar to the original ETBE enrichment (0.31 ± 0.02 mg biomass.mg(-1) ETBE). Among the genes previously described to be involved in ETBE, MTBE, and TBA degradation, only alkB was detected in Rhodococcus sp. IFP 2042 by PCR, and none were detected in Bradyrhizobium sp. IFP 2049. PMID:23474617

Le Digabel, Yoann; Demanèche, Sandrine; Benoit, Yves; Vogel, Timothy M; Fayolle-Guichard, Françoise

2013-12-01

335

Biomolecular techniques to detect Pneumocystis carinii f. sp. hominis pneumonia in patients with acquired immunodeficiency syndrome  

Microsoft Academic Search

Objectives: To verify the clinical value of two different polymerase chain reactions (PCBs) for noninvasive diagnosis and follow-up during Pneumocystis carinii f. sp. hominis pneumonia (PCP) and to analyze the P. carinii f. sp. hominis genotypes involved.Methods: Internal transcribed spacers (ITSs) nested PCR was applied to 630 samples (bronchoalveolar lavage, sera, peripheral blood mononuclear cells, and oropharyngeal samples) from 122

Chiara Atzori; Elena Angeli; Fiorenza Agostoni; Annalisa Mainini; Valeria Micheli; Antonietta Cargnel

1999-01-01

336

Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus)  

PubMed Central

Recently, an undescribed Anaplasma sp. (also called Ehrlichia-like sp. or WTD agent) was isolated in ISE6 tick cells from captive white-tailed deer. The goal of the current study was to characterize this organism using a combination of experimental infection, morphologic, serologic, and molecular studies. Each of 6 experimentally inoculated white-tailed deer fawns (Odocoileus virginianus) became chronically infected (100+ days) with the Anaplasma sp. by inoculation of either infected whole blood or culture. None of the deer showed evidence of clinical disease, but 3 of the 6 deer evaluated had multiple episodes of transient thrombocytopenia. Light microscopy of Giemsa-stained, thin blood smears revealed tiny, dark, spherical structures in platelets of acutely infected deer. Anaplasma sp. was detected in platelets of inoculated deer by polymerase chain reaction, transmission electron microscopy, immunohistochemistry, and in situ hybridization. Five of 6 deer developed antibodies reactive to Anaplasma sp. antigen, as detected by indirect fluorescent antibody testing. Phylogenetic analyses of 16S rRNA, groESL, and gltA sequences confirmed the Anaplasma sp. is related to A. platys. Two attempts to transmit the Anaplasma sp. between deer by feeding Amblyomma americanum, a suspected tick vector, were unsuccessful. Based on its biologic, antigenic, and genetic characteristics, this organism is considered a novel species of Anaplasma, and the name Anaplasma odocoilei sp. nov. is proposed with UMUM76T (=CSUR-A1) as the type strain. PMID:23276749

Tate, Cynthia M.; Howerth, Elizabeth W.; Mead, Daniel G.; Dugan, Vivien G.; Luttrell, M. Page; Sahora, Alexandra I.; Munderloh, Ulrike G.; Davidson, William R.; Yabsley, Michael J.

2014-01-01

337

Genome Sequence of the Microsporidian Species Nematocida sp1 Strain ERTm6 (ATCC PRA-372)  

PubMed Central

Microsporidia comprise a phylum of obligate intracellular pathogens related to fungi. Microsporidia Nematocida sp1 strain ERTm6 was isolated from wild-caught Caenorhabditis briggsae and causes a lethal intestinal infection in Caenorhabditis nematodes. We report the genome sequence of N. sp1 ERTm6, which will facilitate study of the Nematocida genus and other Microsporidia. PMID:25237020

Bakowski, Malina A.; Priest, Margaret; Young, Sarah

2014-01-01

338

Draft Genome Sequence of Geobacillus sp. Strain FW23, Isolated from a Formation Water Sample  

PubMed Central

The thermophilic Geobacillus sp. strain FW23 was isolated from the Mehsana oil wells in Gujrat, India, during a screening for oil-degrading bacteria. Here, we report the draft genome sequence of Geobacillus sp. FW23, which may help reveal the genomic differences between this strain and the earlier reported species of the genus Geobacillus. PMID:24812215

Pore, Soham D.; Arora, Preeti

2014-01-01

339

Candelariella plumbea und C. rhodax sp. novae, zwei neue Arten der europäischen Flechten-Flora  

Microsoft Academic Search

Candelariella plumbea\\u000a Poelt etV?zda sp. n. undC. rhodax\\u000a Poelt etV?zda sp. n., beide unabhängig voneinander von den Autoren gefunden, werden als neu beschrieben. Sie gehören zur Verwandtschaft\\u000a vonC. aurella mit achtsporigen Asci.

Josef Poelt; Antonín V?zda

1976-01-01

340

Consumptive and non-consumptive effects of turbellarian ( Mesostoma sp.) predation on anostracans  

Microsoft Academic Search

We assessed the predatory impact of Mesostoma sp. (Rhabditophora) on hatching and survival of Branchipodopsis wolfi (Anostraca) under laboratory conditions. The hatching percentages of resting eggs of B. wolfi were negatively influenced to a significant degree by the presence of Mesostoma sp. or the medium in which the worms were kept before, but only when prey and predator originated from

Els R. M. De. Roeck; Tom Artois; Luc Brendonck

2005-01-01

341

Consumptive and non-consumptive effects of turbellarian ( Mesostoma sp.) predation on anostracans  

Microsoft Academic Search

We assessed the predatory impact of Mesostoma sp. (Rhabditophora) on hatching and survival of Branchipodopsis wolfi (Anostraca) under laboratory conditions. The hatching percentages of resting eggs of B. wolfi were negatively influenced to a significant degree by the presence of Mesostoma sp. or the medium in which the worms were kept before, but only when prey and predator originated from

Els Roeck; Tom Artois; Luc Brendonck

342

Mycological Society of America Ceratocystiopsis brevicomi sp. nov., a Mycangial Fungus from Dendroctonus brevicomis  

E-print Network

Dendroctonus brevicomis (Coleoptera: Scolytidae) Author(s): Portia Tang-Wung Hsiau and Thomas C. Harrington-5126 Ceratocystiopsisbrevicomi sp. nov., a mycangial fungus from Dendroctonus brevicomis (Coleoptera: Scolytidae) Portia TangMycological Society of America Ceratocystiopsis brevicomi sp. nov., a Mycangial Fungus from

Harrington, Thomas C.

343

Nutritive value of three tropical grasses : Eragrostis olivacea, Setaria sp and Tripsacum laxum, harvested  

E-print Network

. Objective of present study is to evaluate on sheep and cattle the nutritive value of three tropical grassesNutritive value of three tropical grasses : Eragrostis olivacea, Setaria sp and Tripsacum laxum is the main grass found in communal grazing areas. Setaria sp is used as anti- erosive hedge and Tripsacum

Boyer, Edmond

344

1 Estimating aquifer hydraulic properties from the inversion of surface 2 Streaming Potential (SP) anomalies  

E-print Network

1 Estimating aquifer hydraulic properties from the inversion of surface 2 Streaming Potential (SP with the geometry of the water table. It follows that 11 SP measurements can be used to estimate aquifer hydraulic and found that we 14 are able to estimate the hydraulic conductivity and the depth 15 and the thickness

Sailhac, Pascal

345

Draft Genome Sequence of the Shellfish Bacterial Pathogen Vibrio sp. Strain B183  

PubMed Central

We report the draft genome sequence of Vibrio sp. strain B183, a Gram-negative marine bacterium isolated from shellfish that causes mortality in larval mariculture. The availability of this genome sequence will facilitate the study of its virulence mechanisms and add to our knowledge of Vibrio sp. diversity and evolution. PMID:25237023

Schott, Eric J.

2014-01-01

346

SP3X : a six-degree of freedom device for natural model creation  

E-print Network

This thesis presents a novel input device, called SP3X, for the creation of digital models in a semi-immersive environment. The goal of SP3X is to enable novice users to construct geometrically complex three-dimensional ...

Whitney, Richard Henry, III

2007-01-01

347

THE EFFECT OF SALINITY ON EXPERIMENTAL INFECTIONS OF A HEMATODINIUM SP. IN BLUE CRABS, CALLINECTES SAPIDUS  

E-print Network

THE EFFECT OF SALINITY ON EXPERIMENTAL INFECTIONS OF A HEMATODINIUM SP. IN BLUE CRABS, CALLINECTES sp. parasitizes blue crabs along the Atlantic seaboard of the United States. Infections in blue crabs have only been reported from waters where salinity is .11 practical salinity units (psu). Blue crabs

348

Graphene-on-Diamond Devices with Increased Current-Carrying Capacity: Carbon sp2  

E-print Network

Graphene-on-Diamond Devices with Increased Current-Carrying Capacity: Carbon sp2 -on-sp3 Technology Laboratory, Illinois 60439, United States *S Supporting Information ABSTRACT: Graphene demonstrated potential for practical applications owing to its excellent electronic and thermal properties. Typical graphene field

349

Draft Genome Sequence of Hawaiian Sea Slug Symbiont Vibrio sp. Strain ER1A  

PubMed Central

Bacteria belonging to the genus Vibrio are prevalent in the marine environment and are known for forming symbiotic relationships with hosts. Vibrio sp. strain ER1A is a dominant symbiont of the Hawaiian sea slug, Elysia rufescens. Here we report the draft genome sequence of Vibrio sp. ER1A. PMID:25146136

Davis, Jeanette

2014-01-01

350

Draft Genome Sequence of Hawaiian Sea Slug Symbiont Vibrio sp. Strain ER1A.  

PubMed

Bacteria belonging to the genus Vibrio are prevalent in the marine environment and are known for forming symbiotic relationships with hosts. Vibrio sp. strain ER1A is a dominant symbiont of the Hawaiian sea slug, Elysia rufescens. Here we report the draft genome sequence of Vibrio sp. ER1A. PMID:25146136

Davis, Jeanette; Hill, Russell T

2014-01-01

351

[Spatial distribution of fig wasps in syconia of two monoecious Ficus sp].  

PubMed

In addition to pollinator fig wasps, there are several non-pollinating fig wasps associated with monoecious Ficus sp. In order to understand how pollinator fig wasps and non-pollinating fig wasps are distributed across the same syconium, the spatial distribution of fig wasps associated with Ficus altissima and F. benjamina were compared using the pedicle lengths of galls containing each species. The results indicate that in Ficus altissima, the average pedicel length of galls containing Eupristina sp. is longer than that containing E. altissima. Average pedicel length of galls containing Sycobia sp., Micranisa ralianga and Sycoscapter sp. two did not show significant difference. The range of pedicel lengths of galls containing Sycobia sp., M. ralianga or Sycoscapter sp. two is narrower than that of galls containing E. altissima, indicating these non-pollinating fig wasps and pollinator have partially separated spatial niches. In F. benjamina, E. koningsbergeri was distributed in galls from the outer layer to inner layer, while most Walkerella sp. were found in outer layer galls, indicating E. koningsbergeri and Walkerella sp. have partially separated spatial niches. PMID:22467394

Wang, Zhen-Ji; Li, Guo-Chang; Peng, Yan-Qiong; Yang, Da-Rong

2012-04-01

352

Renal coccidiosis caused by Eimeria gaviae n. sp. in a common loon (Gavia immer).  

PubMed

Renal coccidiosis was diagnosed in a common loon (Gavia immer). This is the first report of coccidium from the kidney of a member of the Order Gaviiformes. Morphologically, the coccidium was considered to be an Eimeria sp. distinct from Eimeria truncata, and was named Eimeria gaviae n. sp. PMID:749899

Montgomery, R D; Novilla, M N; Shillinger, R B

1978-01-01

353

Intermediate sp-Hybridization for Bonding Configurations in Nonplanar Covalent Molecules  

E-print Network

1 Intermediate sp-Hybridization for Bonding Configurations in Nonplanar Covalent Molecules sp structure in the C3v-symmetrical C2H6 molecule and the bonding configuration in cluster C60 have been of properties of covalent molecules and solids can be calculated at ease. Correspondence should be sent

Zexian, Cao

354

Metschnikowia proteae sp. nov., a nectarivorous insect-associated yeast species from Africa  

E-print Network

isolated from Heterochelus sp. represent two novel undescribed species (Candida sp. 1 EBDM2Y3 and Candida; Lachance et al., 2006; Rosa et al., 2007). The number of Candida and Metschnikowia species in the Metschnikowia clade has almost quadrupled in the last ten years with 39 Metsch- nikowia species and 26 Candida

Herrera, Carlos M.

355

Comparsion of Selected Growth Media for Culturing Serratia marcescens, Aeromonas sp., and Pseudomonas aeruginosa as  

E-print Network

ARTICLE Comparsion of Selected Growth Media for Culturing Serratia marcescens, Aeromonas sp orally infected with Aeromonas sp., Pseudo- monas aeruginosa (Schroeter), and Serratia marcescens Bizio has examined the activity of a bacteria, Serratia marcescens (Bizio), against stable Ã?ies (Watson

Selinger, Brent

356

Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus).  

PubMed

Recently, an undescribed Anaplasma sp. (also called Ehrlichia-like sp. or WTD agent) was isolated in ISE6 tick cells from captive white-tailed deer. The goal of the current study was to characterize this organism using a combination of experimental infection, morphologic, serologic, and molecular studies. Each of 6 experimentally inoculated white-tailed deer fawns (Odocoileus virginianus) became chronically infected (100+ days) with the Anaplasma sp. by inoculation of either infected whole blood or culture. None of the deer showed evidence of clinical disease, but 3 of the 6 deer evaluated had multiple episodes of transient thrombocytopenia. Light microscopy of Giemsa-stained, thin blood smears revealed tiny, dark, spherical structures in platelets of acutely infected deer. Anaplasma sp. was detected in platelets of inoculated deer by polymerase chain reaction, transmission electron microscopy, immunohistochemistry, and in situ hybridization. Five of 6 deer developed antibodies reactive to Anaplasma sp. antigen, as detected by indirect fluorescent antibody testing. Phylogenetic analyses of 16S rRNA, groESL, and gltA sequences confirmed the Anaplasma sp. is related to A. platys. Two attempts to transmit the Anaplasma sp. between deer by feeding Amblyomma americanum, a suspected tick vector, were unsuccessful. Based on its biologic, antigenic, and genetic characteristics, this organism is considered a novel species of Anaplasma, and the name Anaplasma odocoilei sp. nov. is proposed with UMUM76(T) (=CSUR-A1) as the type strain. PMID:23276749

Tate, Cynthia M; Howerth, Elizabeth W; Mead, Daniel G; Dugan, Vivien G; Luttrell, M Page; Sahora, Alexandra I; Munderloh, Ulrike G; Davidson, William R; Yabsley, Michael J

2013-02-01

357

Cozumel Island fox (Urocyon sp.) dwarfism and possible divergence history based on subfossil bones  

E-print Network

Cozumel Island fox (Urocyon sp.) dwarfism and possible divergence history based on subfossil bones fox (Urocyon sp.) has inhabited Cozumel Island, Mexico at least since the arrival of Mayan peoples that this fox is on the verge of extinction and therefore it is critical to describe the population. Because

Gompper, Matthew E.

358

Power plant system assessment. Final report. SP-100 Program  

SciTech Connect

The purpose of this assessment was to provide system-level insights into 100-kWe-class space reactor electric systems. Using these insights, Rockwell was to select and perform conceptual design studies on a ''most attractive'' system that met the preliminary design goals and requirements of the SP-100 Program. About 4 of the 6 months were used in the selection process. The remaining 2 months were used for the system conceptual design studies. Rockwell completed these studies at the end of FY 1983. This report summarizes the results of the power plant system assessment and describes our choice for the most attractive system - the Rockwell SR-100G System (Space Reactor, 100 kWe, Growth) - a lithium-cooled UN-fueled fast reactor/Brayton turboelectric converter system.

Anderson, R.V.; Atkins, D.F.; Bost, D.S.; Berman, B.; Clinger, D.A.; Determan, W.R.; Drucker, G.S.; Glasgow, L.E.; Hartung, J.A.; Harty, R.B.

1983-10-31

359

Pulmonary trematodosis (Pharyngostomoides sp.) in a juvenile raccoon (Procyon lotor).  

PubMed

A lethargic, wild, juvenile raccoon died and was subsequently presented for necropsy. The lungs had widely disseminated, finely granular, yellow-tan foci with moderate pulmonary, as well as, thoracic lymphadenopathy. Histopathology revealed numerous cross-sections of larval trematodes morphologically consistent with diplostomid mesocercariae and metacercariae as well as moderate alveolointerstitial pneumonia with a mixed population of eosinophils, macrophages, lymphocytes, and plasma cells. The presence of larval trematodes within lymphatics in multiple organs indicates a route of fluke migration. Live specimens of mesocercariae and metacercariae recovered from refrigerated lung tissue after 7 days were identified as Pharyngostomoides sp., an intestinal diplostomid parasite (trematode) of raccoons and other mammals that is similar in appearance and life cycle to Alaria spp. PMID:21908291

Olson, Erik J; Conboy, Gary A; Stromberg, Bert E; Hayden, David W

2011-05-01

360

Complete genome sequence of Arthrobacter sp. strain FB24  

SciTech Connect

Arthrobacter sp. strain FB24 is a species in the genus Arthrobacter Conn and Dimmick 1947, in the family Micrococcaceae and class Actinobacteria. A number of Arthrobacter genome sequences have been completed because of their important role in soil, especially bioremediation. This isolate is of special interest because it is tolerant to multiple metals and it is extremely resistant to elevated concentrations of chromate. The genome consists of a 4,698,945 bp circular chromosome and three plasmids (96,488, 115,507, and 159,536 bp, a total of 5,070,478 bp), coding 4,536 proteins of which 1,257 are without known function. This genome was sequenced as part of the DOE Joint Genome Institute Program.

Nakatsu, C. H.; Barabote, Ravi; Thompson, Sue; Bruce, David; Detter, Chris; Brettin, T.; Han, Cliff F.; Beasley, Federico; Chen, Weimin; Konopka, Allan; Xie, Gary

2013-09-30

361

Bioactive Compounds from a Gorgonian Coral Echinomuricea sp. (Plexauridae)  

PubMed Central

A new labdane-type diterpenoid, echinolabdane A (1), and a new sterol, 6-epi-yonarasterol B (2), were isolated from a gorgonian coral identified as Echinomuricea sp. The structures of metabolites 1 and 2 were elucidated by spectroscopic methods. Echinolabdane A (1) possesses a novel tetracyclic skeleton with an oxepane ring jointed to an ?,?-unsaturated-?-lactone ring by a hemiketal moiety, and this compound is the first labdane-type diterpenoid to be obtained from marine organisms belonging to the phylum Cnidaria. 6-epi-Yonarasterol B (2) is the first steroid derivative to be isolated from gorgonian coral belonging to the genus Echinomuricea, and this compound displayed significant inhibitory effects on the generation of superoxide anions and the release of elastase by human neutrophils. PMID:22822364

Chung, Hsu-Ming; Hong, Pei-Han; Su, Jui-Hsin; Hwang, Tsong-Long; Lu, Mei-Chin; Fang, Lee-Shing; Wu, Yang-Chang; Li, Jan-Jung; Chen, Jih-Jung; Wang, Wei-Hsien; Sung, Ping-Jyun

2012-01-01

362

Facklamia languida sp. nov., Isolated from Human Clinical Specimens  

PubMed Central

Three strains of a gram-positive catalase-negative, facultatively anaerobic coccus-shaped organism originating from human clinical samples were characterized by phenotypic and molecular taxonomic methods. Sequencing of genes encoding 16S rRNA showed that the strains are phylogenetically closely related (99.9 to 100% sequence similarity) and represent a new subline within the genus Facklamia. The unknown bacterium was readily distinguished from all currently described species of the genus Facklamia (viz., Facklamia hominis, Facklamia ignava, and Facklamia sourekii) by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Facklamia languida sp. nov. The type strain of F. languida is CCUG 37842. PMID:10074542

Lawson, Paul A.; Collins, Matthew D.; Falsen, Enevold; Sjoden, Berit; Facklam, Richard R.

1999-01-01

363

Gemella bergeriae sp. nov., Isolated from Human Clinical Specimens  

PubMed Central

Six strains of a hitherto-undescribed gram-positive, catalase-negative, facultatively anaerobic coccus from human sources were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown strains are genealogically homogeneous and constitute a new subline within the genus Gemella. The unknown bacterium was readily distinguished from Gemella haemolysans, the type species of the genus Gemella, and from Gemella morbillorum by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from clinical specimens be classified as Gemella bergeriae sp. nov. The type strain of G. bergeriae is CCUG 37817 (= strain 617-93). PMID:9574693

Collins, Matthew D.; Hutson, Roger A.; Falsen, Enevold; Sjoden, Berit; Facklam, Richard R.

1998-01-01

364

A Microbial Metagenome (Leucobacter sp.) in Caenorhabditis Whole Genome Sequences  

PubMed Central

DNA of apparently recent bacterial origin is found in the genomic sequences of Caenorhabditis angaria and Caenorhabditis remanei. Here we present evidence that the DNA belongs to a single species of the genus Leucobacter (high-GC Gram+ Actinobacteria). Metagenomic tools enabled the assembly of the contaminating sequences in a draft genome of 3.2 Mb harboring 2,826 genes. This information provides insight into a microbial organism intimately associated with Caenorhabditis as well as a solid basis for the reassignment of 3,373 metazoan entries of the public database to a novel bacterial species (Leucobacter sp. AEAR). The application of metagenomic techniques can thus prevent annotation errors and reveal unexpected genetic information in data obtained by conventional genomics. PMID:23585714

Percudani, Riccardo

2013-01-01

365

Degradation of 2-chlorotoluene by Rhodococcus sp. OCT 10.  

PubMed

A strain Rhodococcus sp. OCT 10 DSM 45596(T), exhibiting 99.9% of 16S rDNA identity with Rhodococcus wratislaviensis NCIMB 13082, was isolated from a soil sample. The strain completely mineralised 2-chlorotoluene, 2-bromotoluene, o-xylene, benzyl alcohol and benzoate. In contrast, 2-fluorotoluene was only partially mineralised. By GC-MS and (1)H-NMR analyses, 4-chloro-3-methylcatechol was identified as the central intermediate in the degradation pathway of 2-chlorotoluene. It was further degraded by enzymes of the meta cleavage pathway. Catechol 1,2-dioxygenase and chlorocatechol 1,2-dioxygenase as the initial enzymes of the ortho cleavage pathways were not detectable under these conditions. Furthermore, neither formation nor oxidation of 2-chlorobenzylic alcohol, 2-chlorobenzaldehyde, or 2-chlorobenzoate was observed, thereby excluding side chain oxidation activity. PMID:21870046

Dobslaw, Daniel; Engesser, Karl-Heinrich

2012-03-01

366

Failure Analysis of Nonvolatile Residue (NVR) Analyzer Model SP-1000  

NASA Technical Reports Server (NTRS)

National Aeronautics and Space Administration (NASA) subcontractor Wiltech contacted the NASA Electrical Lab (NE-L) and requested a failure analysis of a Solvent Purity Meter; model SP-IOOO produced by the VerTis Instrument Company. The meter, used to measure the contaminate in a solvent to determine the relative contamination on spacecraft flight hardware and ground servicing equipment, had been inoperable and in storage for an unknown amount of time. NE-L was asked to troubleshoot the unit and make a determination on what may be required to make the unit operational. Through the use of general troubleshooting processes and the review of a unit in service at the time of analysis, the unit was found to be repairable but would need the replacement of multiple components.

Potter, Joseph C.

2011-01-01

367

Desulfurization of thianthrene by a Gordonia sp. IITR100.  

PubMed

Thianthrene (TA) was desulfurized by an isolated strain, Gordonia sp. IITR100. The reaction is accompanied with the formation of TA-sulfoxide, TA-sulfone and 2-phenylsulfanylphenol. The formed 2-phenylsulfanylphenol undergoes further oxidation to o-hydroxyphenyl phenylsulfone that accumulates as an end product. Metabolism of TA to TA-sulfone can also occur by E. coli-DszC i.e. E. coli cells that were harboring the gene coding for the enzyme dibenzothiophene desulfurase C. When presented to E. coli-DszC in a binary combination with dibenzothiophene, TA metabolism was completely inhibited. Metabolism of TA-TA-sulfone by E. coli-DszC, as well as the nature of metabolites formed by IITR100, suggests that the desulfurization pathway for TA is similar to that of the thiophenic compounds. This is first report on the desulfurization of thianthrene, and has implications on biodesulfurization when multiple sulfur compounds are present together. PMID:25113826

Ahmad, Abrar; Chauhan, Ashok Kumar; Javed, Saleem; Kumar, Ashwani

2014-11-01

368

Streptomyces graminifolii sp. nov., isolated from bamboo (Sasa borealis) litter.  

PubMed

The taxonomic position of strain JL-22(T), isolated from litter of a bamboo (Sasa borealis) forest, was determined using a polyphasic approach. The organism had phenotypic and morphological properties consistent with it being a member of the genus Streptomyces. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain JL-22(T) was closely related to Streptomyces prunicolor NRRL B-12281(T) (99.2%), Streptomyces galilaeus JCM 4757(T) (99.0%) and Streptomyces chartreusis NBRC 12753(T) (99.0%). However, the results of DNA-DNA hybridization and physiological and biochemical tests showed that strain JL-22(T) could be differentiated from its closest phylogenetic relatives both genotypically and phenotypically. Based on phenotypic and genotypic data, strain JL-22(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces graminifolii sp. nov. is proposed. The type strain is JL-22(T) (?=?KACC 17180(T)?=?NBRC 109806(T)). PMID:24812360

Lee, Hyo-Jin; Whang, Kyung-Sook

2014-08-01

369

Pseudoxanthomonas suwonensis sp. nov., isolated from cotton waste composts.  

PubMed

Three strains, 4M1T, 4M9 and 4M12, were isolated from cotton waste composts. These strains are Gram-negative, aerobic and non-spore-forming rods. 16S rRNA gene sequence comparisons demonstrated that these isolates were clustered phylogenetically within the genus Pseudoxanthomonas and 4M1T revealed sequence similarity levels of 96.9-99.0% to six Pseudoxanthomonas species with validly published names. According to DNA-DNA hybridization, relatedness values between 4M1T and six known Pseudoxanthomonas species were in the range of 52-63%. The DNA G + C content of the strains was 66.6-68.4 mol%. For a more detailed characterization of these strains, the physiological, chemotaxonomic and genotypic properties were evaluated. From the results of this study, the name Pseudoxanthomonas suwonensis sp. nov. is proposed, with the type strain 4M1T (= KACC 11320T = DSM 17175T). PMID:16514045

Weon, Hang-Yeon; Kim, Byung-Yong; Kim, Jong-Shik; Lee, Seon-Young; Cho, Yang-Hee; Go, Seung-Joo; Hong, Seung-Beom; Im, Wan-Taek; Kwon, Soon-Wo

2006-03-01

370

Nocardia aciditolerans sp. nov., isolated from a spruce forest soil.  

PubMed

Actinomycetes growing on acidified starch-casein agar seeded with suspensions of litter and mineral soil from a spruce forest were provisionally assigned to the genus Nocardia based upon colonial properties. Representative isolates were found to grow optimally at pH 5.5, have chemotaxonomic and morphological features consistent with their assignment to the genus Nocardia and formed two closely related subclades in the Nocardia 16S rRNA gene tree. DNA:DNA relatedness assays showed that representatives of the subclades belong to a single genomic species. The isolates were distantly associated with their nearest phylogenetic neighbour, the type strain of Nocardia kruczakiae, and were distinguished readily from the latter based on phenotypic properties. On the basis of these data it is proposed that the isolates merit recognition as a new species, Nocardia aciditolerans sp. nov. The type strain is isolate CSCA68(T) (=KACC 17155(T) = NCIMB 14829(T) = DSM 45801(T)). PMID:23371034

Golinska, Patrycja; Wang, Dylan; Goodfellow, Michael

2013-05-01

371

Antifouling activities of marine bacteria associated with sponge ( Sigmadocia sp.)  

NASA Astrophysics Data System (ADS)

The present study aimed at assessing the antifouling activity of bacteria associated with marine sponges. A total of eight bacterial strains were isolated from the surface of sponge Sigmadocia sp., of them, SS02, SS05 and SS06 showed inhibitory activity against biofilm-forming bacteria. The extracts of these 3 strains considerably affected the extracellular polymeric substance producing ability and adhesion of biofilm-forming bacterial strains. In addition to disc diffusion assay, microalgal settlement assay was carried out with the extracts mixed with polyurethane wood polish and coated onto stainless steel coupons. The extract of strain SS05 showed strong microalgal settlement inhibitory activity. Strain SS05 was identified as Bacillus cereus based on its 16S rRNA gene. Metabolites of the bacterial strains associated with marine invertebrates promise to be developed into environment-friendly antifouling agents.

Satheesh, S.; Soniamby, A. R.; Sunjaiy Shankar, C. V.; Mary Josephine Punitha, S.

2012-09-01

372

Orthorhombic C32: a novel superhard sp3 carbon allotrope.  

PubMed

Using a recently developed 'Crystal structure AnaLYsis by Particle Swarm Optimization' (CALYPSO) algorithm on a structural search, we predicted a novel sp(3) carbon allotrope possessing an orthorhombic lattice with the space group Cmmm (oC32). The calculated elastic constants and the simulated hardness revealed that oC32 simultaneously possesses ultra-incompressible and superhard properties with a high bulk modulus of 457 GPa and a high Vickers hardness of 96.2 GPa. This oC32 phase is dynamically stable and energetically more preferable than the experientially observed cold-compressed carbon, thus oC32 is expected to be experimentally synthesizable under extreme conditions. These results further expand the list of meta-stable carbon allotropes and superhard materials under atmospheric and extreme conditions. PMID:23872724

Zhang, Miao; Liu, Hanyu; Du, Yonghui; Zhang, Xinxin; Wang, Yanchao; Li, Quan

2013-09-01

373

Photobacterium swingsii sp. nov., isolated from marine organisms.  

PubMed

Six Gram-negative coccobacilli were isolated from Pacific oysters (Crassostrea gigas) from Mexico and haemolymph of spider crabs (Maja brachydactyla) from Spain. All of the isolates grew as small green colonies on thiosulphate-citrate-bile salts-sucrose (TCBS) agar and were facultatively anaerobic, oxidase-positive and sensitive to the vibriostatic agent O/129. Repetitive palindromic PCR analysis revealed a high degree of genomic homogeneity among the isolates. Several phenotypic traits differentiated the isolates from the type strains of species of the genus Photobacterium. DNA-DNA relatedness between two representative isolates and their closest phylogenetic neighbours by 16S rRNA gene sequence similarity, Photobacterium aplysiae CAIM 14(T) and Photobacterium frigidiphilum CAIM 20(T), was 44.01-53.85?%. We propose a novel species of the genus Photobacterium to accommodate the six isolates, with the name Photobacterium swingsii sp. nov. The type strain is CAIM 1393(T) (=CECT 7576(T)). PMID:20228205

Gomez-Gil, Bruno; Roque, Ana; Rotllant, Guiomar; Peinado, Lauro; Romalde, Jesus L; Doce, Alejandra; Cabanillas-Beltrán, Hector; Chimetto, Luciane A; Thompson, Fabiano L

2011-02-01

374

Wavelength modulation of phycoerythrin synthesis in Synechocystis sp. 6701.  

PubMed Central

The spectral dependence of phycoerythrin synthesis has been studied in a unicellular photautotrophic cyanobacterium, Synechocystis sp. 6701, in which phycoerythrin synthesis alone is under chromatic control. Cells were partially depleted of their phycobiliprotein pigments through nitrate starvation in the light. Addition of nitrate to the culture medium allowed synthesis of phycobiliproteins in the dark. This synthesis occurred at the expense of the glycogen reserve accumulated during the period of nitrate starvation. Monochromatic irradiations of short duration at lambda less than 590 nm induced increased phycoerythrin synthesis during dark incubation. Monochromatic irradiations of short duration at lambda greater than 590 nm prevented this synthesis. These effects were photoreversible. The spectral distribution showed a maximum at 540 nm for the potentiation of phycoerythrin synthesis, and one at 640 nm for its photoreversal. PMID:6768711

de Marsac, N T; Castets, A M; Cohen-Bazire, G

1980-01-01

375

Isoptericola chiayiensis sp. nov., isolated from mangrove soil.  

PubMed

A novel actinomycete, designated strain 06182M-1(T), was isolated from a mangrove soil sample collected from Chiayi County in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0-98.8?% to the type strains of recognized species of the genus Isoptericola. Chemotaxonomic data also supported the placement of strain 06182M-1(T) within the genus Isoptericola. However, the low levels of DNA-DNA relatedness between the novel strain and the type strains of recognized species of the genus Isoptericola, in combination with differential phenotypic data, demonstrate that strain 06182M-1(T) represents a novel species of the genus Isoptericola, for which the name Isoptericola chiayiensis sp. nov. is proposed. The type strain is 06182M-1(T) (?=?BCRC 16888(T) ?=?KCTC 19740(T)). PMID:20729312

Tseng, M; Liao, H C; Chiang, W P; Yuan, G F

2011-07-01

376

Stability of haloalkaliphilic Geomicrobium sp. protease modulated by salt.  

PubMed

A novel protease from the halophilic bacterium Geomicrobium sp. EMB2 (MTCC 10310) is described. The activity of the protease was modulated by salt, and it exhibited remarkable stability in organic solvents, at alkaline pH, and in other denaturing conditions. The structural changes under various denaturing conditions were analyzed by measurements of intrinsic fluorescence and circular dichroism spectroscopy. Circular dichroism showed that the secondary structure of the protease was predominantly ?-helical but unfolded in salt-free medium. The structure is regained by inclusion of NaCl in the range of 2-5%. The presence of NaCl exerted a protective effect against thermal, organic solvent, and guanidine hydrochloride denaturation by preventing unfolding. PMID:21639849

Karan, R; Khare, S K

2011-06-01

377

Metschnikowia orientalis sp. nov., an Australasian yeast from nitidulid beetles.  

PubMed

A novel species, Metschnikowia orientalis sp. nov., is described for haploid, heterothallic yeasts isolated from nitidulid beetles sampled in flowers in Rarotonga in the Cook Islands, and the Cameron Highlands of Malaysia. As evidenced by analysis of D1/D2 large subunit rDNA sequences, the species is related to Candida hawaiiana, to which it is similar in growth responses. Cylindrical, conjugated asci and acicular ascospores of moderate size are formed. Rudimentary mating reactions were observed with Metschnikowia aberdeeniae and Metschnikowia continentalis, but not with C. hawaiiana. The type strain of M. orientalis is UWOPS 99-745.6(T) (h(+)) (=CBS 10331(T)=NRRL Y-27991(T)) and the designated allotype is UWOPS 05-269.1 (h(-)) (=CBS 10330=NRRL Y-27992). PMID:17012584

Lachance, Marc-André; Bowles, Jane M; Wiens, Frank; Dobson, Jessica; Ewing, Curtis P

2006-10-01

378

Coniosporium epidermidis sp. nov., a new species from human skin  

PubMed Central

Coniosporium epidermidis sp. nov. is described from a superficial skin lesion with blackish discolouration in an 80-yr-old Chinese patient. The species produces dark, thick-walled, inflated, reluctantly liberating arthroconidia without longitudinal septa. Sequences of the ribosomal operon, as well as of the translation elongation factor 1-? support its novelty. The species is found in a lineage basal to the order Chaetothyriales, amidst relatives from rock, but also species repeatedly isolated from human skin and nails and eventually causing mild cutaneous infections. Coniosporium epidermidis is consistently found on humans, either asymptomatic or symptomatic. The species indicates a change of life style towards human pathogenicity, which is a recurrent type of ecology in derived Chaetothyriales. Superficial and cutaneous infection by melanized fungi is a new category in dermatology. PMID:19287535

Li, D. M.; de Hoog, G.S.; Saunte, D.M. Lindhardt; van den Ende, A.H.G. Gerrits; Chen, X. R.

2008-01-01

379

Prauserella shujinwangii sp. nov., from a desert environment.  

PubMed

A Gram-positive, spore-forming, rod-shaped actinomycete, designated XJ46(T), was isolated from Xinjiang Uyghur Autonomous Region, China and subjected to a polyphasic taxonomic analysis. Morphological and chemotaxonomic characteristics of XJ46(T) were identified as being similar to those of members of the genus Prauserella. The phylogenetic tree based on 16S rRNA gene sequences showed that XJ46(T) shared the highest similarity (95.9?%) with Prauserella marina MS498(T). Based on its phenotypic characteristics, chemotaxonomic analysis and 16S rRNA gene sequence analysis, strain XJ46(T) is proposed to represent a novel species of the genus Prauserella, named Prauserella shujinwangii sp. nov. The type strain is XJ46(T) (?=?CGMCC 4.7125(T)?=?JCM 19736(T)). PMID:25158847

Liu, Mei; Zhang, Li; Ren, Biao; Yang, Na; Yu, Xiaoyun; Wang, Jian; Ding, Linxian; Liu, Xueting; Liu, Zhiheng; Goodfellow, Michael; Zhang, Lixin

2014-11-01

380

Arcanobacterium hippocoleae sp. nov., from the vagina of a horse.  

PubMed

A polyphasic taxonomic study was performed on a previously unidentified gram-positive, facultatively anaerobic, diphtheroid-shaped organism isolated from a vaginal discharge of a horse. Comparative 16S rRNA gene sequencing demonstrated that the strain was a member of the genus Arcanobacterium, but sequence divergence values of >4% with described species of this genus (viz: Arcanobacterium haemolyticum, Arcanobacterium bernardiae, Arcanobacterium phocae, Arcanobacterium pluranimalium and Arcanobacterium pyogenes) demonstrated that the isolate represented a novel species. The unknown bacterium was readily distinguished from other Arcanobacterium species by biochemical tests. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Arcanobacterium hippocoleae sp. nov. The type strain of A. hippocoleae is CCUG 44697T (= CIP 106850T). PMID:11931175

Hoyles, Lesley; Falsen, Enevold; Foster, Geoffrey; Rogerson, Forbes; Collins, Matthew D

2002-03-01

381

Mucopolysaccharidosis Type II and the G374sp Mutation  

PubMed Central

Mucopolysaccharidosis type II (MPS II), also known as Hunter syndrome, is a rare, X-linked disease caused by a deficiency of the lysosomal enzyme iduronate-2-sulfatase, which catalyses a step in the catabolism of glycosaminoglycans resulting in accumulation of heparan and dermatan sulfate in many organs and tissues. This accumulation favors the appearance of neurologic involvement, severe airway obstruction, skeletal deformities, and cardiomyopathy, especially mitral and aortic valve regurgitation. In severe cases, obstructive airway disease and cardiac failure due to valvular dysfunction are the most common causes of death within the second decade of life. However, in mild cases, intelligence remains normal, stature is almost normal and death usually occurs due to cardiac failure in the fourth decade of life. We report the presentation, diagnosis, management, and outcome of 2 siblings with MPS II and the G374sp mutation at the nucleotide c.1246 of the gene encoding for the iduronate-2-sulfatase. PMID:23801937

Martinez-Quintana, E.; Rodriguez-Gonzalez, F.

2013-01-01

382

Growth of Scytalidium sp. in a counterfeit bevacizumab bottle  

PubMed Central

After drawing a dose from an closed bevacizumab (Avastin) bottle, a fungus-like foreign body was observed inside. Samples from the vial were cultured in Sabouraud Emmons media. Growth of multiple light brown colonies with dark pigment was observed after 10 days. The species was identified as Scytalidium sp. Vial, analysis reported that the seal was lacking proper identification measures and that the label, batch number and expiry date did not correspond to a genuine product. Chemical analysis showed no protein, but 3% of polyethylene glycol, citrate and ethanol. Counterfeit bevacizumab is a real situation that poses a significant risk for ophthalmology and oncology patients. The medical community should be aware of this situation in order to enforce adequate preventive measures. PMID:24104716

Garcia-Aguirre, Gerardo; Vanzinni-Zago, Virginia; Quiroz-Mercado, Hugo

2013-01-01

383

Cytotoxic Metabolites from an Indonesian Sponge Lendenfeldia sp  

PubMed Central

The lipid extract of an Indonesian Lendenfeldia sp. sponge inhibited hypoxia-induced hypoxia-inducible factor-1 (HIF-1) activation in T47D breast tumor cells. Chromatographic separation yielded the new substituted naphthalene dimer 1, the new furanolipid 2, and three known homoscalarane sestertepenes 3 - 5. Compounds 1 and 3 - 5 inhibited hypoxia-induced HIF-1 activation (IC50 values: 0.64 - 6.9 ?M), but also reduced the viability of T47D and MDA-MB-231 breast tumor cells. Compound 4 was the most potent and showed a unique tumor cell line selectivity in the NCI 60-cell line panel. The general cytotoxicity of these compounds precluded their further consideration as HIF-1 inhibitors. Compound 1 Hypoxia-induced HIF-1 inhibition: IC50 4.6 ?M T47D Breast tumor cell proliferation/viability (16 h): IC50 19 ?M PMID:17958396

Dai, Jingqiu; Liu, Yang; Zhou, Yu-Dong; Nagle, Dale G.

2009-01-01

384

SP-100 W/Re thermocouple calibration techniques  

SciTech Connect

The accuracy requirement of the SP-100 Temperature Sensor Program for the reference tungsten/rhenium metal-sheathed thermocouple sensor is {plus minus}1.4% of the reading (in Kelvin) at space reactor operating temperature (nominally 1375 K) over the operating life of the reactor. This is a stringent requirement considering that the as-fabricated base wire limits of error'' are generally specified at roughly {plus minus}1.0% for exposure temperatures above 700 K. Although the thermocouples will be located out of the reactor's neutron field so that irradiation decalibration is expected to be negligible, thermal exposure effects will probably cause the thermocouple to drift out of specification during the 10-yr reactor operating life. A plan to accurately quantify thermocouple drift with aging time, and thus provide on-board calibration for the sensor is described.

Cannon, N.S.; Knight, R.C.

1992-01-01

385

Cladieunicellins M-Q, New Eunicellins from Cladiella sp.  

PubMed Central

Five new 7?-hydroxyeunicellin-based diterpenoids, designated as cladieunicellins M–Q (1–5), were isolated from a Formosan octocoral Cladiella sp. The structures of 1–5 were elucidated on the basis of spectroscopic methods and by comparison of the data with those of the related metabolites. Cytotoxicity of metabolites 1–5 against the human leukemia Molt 4 and HL 60 is also described. Among them, compounds 1, 3 and 5 exhibited moderate cytotoxicity toward Molt 4 cells with IC50 values 16.43, 14.17 and 15.55 ?M, respectively. Preliminary SAR (structure activity relationship) information was obtained from these compounds and their analogues. PMID:24717524

Chen, Tsung-Hung; Chen, Wu-Fu; Wen, Zhi-Hong; Lu, Mei-Chin; Wang, Wei-Hsien; Li, Jan-Jung; Wu, Yang-Chang; Sung, Ping-Jyun

2014-01-01

386

Lactobacillus pobuzihii sp. nov., isolated from pobuzihi (fermented cummingcordia).  

PubMed

Twenty-one homofermentative lactic acid bacteria were isolated from fermented cummingcordia (pobuzihi), a traditional food in Taiwan. The isolates had identical 16S rRNA gene sequences that were distinct from those of other lactobacilli, and their closest neighbours in the 16S rRNA gene sequence phylogenetic tree were strains of Lactobacillus acidipiscis. Levels of DNA-DNA relatedness between representative pobuzihi isolates and strains of L. acidipiscis were 17% and below. Furthermore, the new isolates could be differentiated clearly from L. acidipiscis NBRC 102163T and NBRC 102164 in terms of acid production from L-arabinose, rhamnose, mannitol, lactose and 5-ketogluconate. It was concluded that the new isolates represent a single novel species of the genus Lactobacillus, for which the name Lactobacillus pobuzihii sp. nov. is proposed. The type strain is E100301T (=RIFY 6501T =NBRC 103219T =KCTC 13174T). PMID:19783610

Chen, Yi-Sheng; Miyashita, Mika; Suzuki, Ken-ichiro; Sato, Hajime; Hsu, Jar-Sheng; Yanagida, Fujitoshi

2010-08-01

387

Weissella fabaria sp. nov., from a Ghanaian cocoa fermentation.  

PubMed

Two lactic acid bacteria, strains 257(T) and 252, were isolated from traditional heap fermentations of Ghanaian cocoa beans. 16S rRNA gene sequence analysis of these strains allocated them to the genus Weissella, showing 99.5 % 16S rRNA gene sequence similarity towards Weissella ghanensis LMG 24286(T). Whole-cell protein electrophoresis, fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and biochemical tests confirmed their unique taxonomic position. DNA-DNA hybridization experiments towards their nearest phylogenetic neighbour demonstrated that the two strains represent a novel species, for which we propose the name Weissella fabaria sp. nov., with strain 257(T) (=LMG 24289(T) =DSM 21416(T)) as the type strain. Additional sequence analysis using pheS gene sequences proved useful for identification of all Weissella-Leuconostoc-Oenococcus species and for the recognition of the novel species. PMID:19801391

De Bruyne, Katrien; Camu, Nicholas; De Vuyst, Luc; Vandamme, Peter

2010-09-01

388

Alkalibacterium thalassium sp. nov., Alkalibacterium pelagium sp. nov., Alkalibacterium putridalgicola sp. nov. and Alkalibacterium kapii sp. nov., slightly halophilic and alkaliphilic marine lactic acid bacteria isolated from marine organisms and salted foods collected in Japan and Thailand.  

PubMed

We describe 10 new strains of marine lactic acid bacteria isolated from decaying marine algae, decaying seagrass, raw fish, salted fish and salted and fermented shrimp paste ('ka-pi') collected from a temperate area of Japan and Thailand. The isolates are Gram-positive and non-sporulating. They have motility with peritrichous flagella depending on the strains. They lack catalase and quinones. Under anaerobic conditions, lactate yields were 64-93 % of the glucose consumed; residual products were formate, acetate and ethanol with a molar ratio of approximately 2 : 1 : 1. The pH of the fermentation medium markedly affected the product composition; at higher pH, the yield of lactate decreased (15-48 % at pH 9.0) and yields of other products increased, retaining the molar ratio. Under aerobic conditions, acetate and lactate were produced from carbohydrates and related compounds. The isolates were slightly halophilic, highly halotolerant and alkaliphilic. The optimum NaCl concentration for growth ranged between 0.5 and 4.0 % (w/v), depending on the strain, with a growth range of between 0 and 17-21 % (11 % for one isolate). The optimum pH for growth ranged between 8.0 and 9.5, with a growth range of 6.0-11.0, depending on the strains. Comparative sequence analysis of the 16S rRNA genes revealed that the isolates occupied three phylogenetic positions within the genus Alkalibacterium, showing 97.1-99.8 % similarity to Alkalibacterium indicireducens. DNA-DNA hybridization values (<46 %) among the 10 isolates and phylogenetically related taxa resulted in the identification of four genomic species (designated groups GS1-GS4). The G+C contents of the DNA were 41.7 mol% (group GS1), 42.2 mol% (group GS2), 41.0-43.0 mol% (group GS3) and 38.4-39.4 mol% (group GS4). The cell-wall peptidoglycan was type A4beta, Orn-d-Asp, for three genomic species (groups GS1, GS2 and GS3), and type A4beta, Orn-d-Glu, for the remaining species (group GS4). The major components of cellular fatty acids were C(16 : 0), C(16 : 1)omega9c and C(18 : 1)omega9c (oleic acid). On the bases of phenotypic characteristics, genetic distinctiveness and phylogenetic affiliations, the four genomic species, groups GS1, GS2, GS3 and GS4, were classified as four novel species within the genus Alkalibacterium, for which the names Alkalibacterium thalassium sp. nov., Alkalibacterium pelagium sp. nov., Alkalibacterium putridalgicola sp. nov. and Alkalibacterium kapii sp. nov., respectively, are proposed. The respective type strains are T117-1-2(T) (=DSM 19181(T)=NBRC 103241(T)=NRIC 0718(T)), T143-1-1(T) (=DSM 19183(T)=NBRC 103242(T)=NRIC 0719(T)), T129-2-1(T) (=DSM 19182(T)=NBRC 103243(T)=NRIC 0720(T)) and T22-1-2(T) (=DSM 19180(T)=NBRC 103247(T)=NRIC 0724(T)). PMID:19406822

Ishikawa, Morio; Tanasupawat, Somboon; Nakajima, Kazuyuki; Kanamori, Hajime; Ishizaki, Shihomi; Kodama, Kayo; Okamoto-Kainuma, Akiko; Koizumi, Yukimichi; Yamamoto, Yasushi; Yamasato, Kazuhide

2009-05-01

389

Treatment of Argulus sp. infestation of river frogs.  

PubMed

Ten river frog tadpoles (Rana hecksheri) were collected from Pointsett State Park in South Carolina in April 1995. They were housed together in a tank at the North Carolina Zoological Park. Although no skin lesions were evident at collection, skin scrapings performed 4 weeks later revealed numerous immature and adult Argulus sp. on the tails and dorsal trunks of many of the tadpoles. The adult parasites were removed manually, and the tadpoles were treated with lufenuron (15 mg/l; Program, Novartis Animal Health, Greensboro, N.C.) and sodium chloride (3 g/l) in the tank water for 3 weeks. A single immature Argulus was found on a skin scraping on day 2 of treatment, and no parasites were seen thereafter on skin scrapings obtained through day 28 after the initiation of treatment. Metamorphosis occurred in all tadpoles within 4 weeks of initiating treatment. No deleterious effects of the treatment were noted. PMID:11703056

Wolfe, B A; Harms, C A; Groves, J D; Loomis, M R

2001-11-01

390

Geotrichum vulgare sp. nov., a novel asexual arthroconidial yeast.  

PubMed

Two strains of a novel yeast species were isolated from different habitats, from soil in an alluvial zone national park in Austria and from a drain in a Turkish soft drinks factory. Analysis of the nucleotide sequences of the D1/D2 region of their large-subunit rDNAs and PCR fingerprints show that the strains are members of the same species, described as Geotrichum vulgare sp. nov. Analysis of nucleotide sequences showed that this species is related to the ascogenous genus Galactomyces. The closest phylogenetic relative is Geotrichum silvicola, a recently described species. The type strain of Geotrichum vulgare is HA1379T (= CBS 10073T = NRRL Y-27915T). PMID:16403902

Wuczkowski, Michael; Bond, Chris; Prillinger, Hansjörg

2006-01-01

391

Peptostreptococcus stomatis sp. nov., isolated from the human oral cavity.  

PubMed

Seven strains of anaerobic Gram-positive cocci isolated from human oral sites were subjected to a comprehensive range of phenotypic and genotypic tests. 16S rRNA gene sequence analysis revealed that the strains constituted a homogeneous group that was distinct from species with validly published names, but related to Peptostreptococcus anaerobius. All oral strains tested belonged to this group, whereas all non-oral strains studied were confirmed as P. anaerobius. A novel species, Peptostreptococcus stomatis sp. nov., is proposed to accommodate these oral strains. P. stomatis is weakly saccharolytic and produces acetic, butyric, isobutyric, isovaleric and isocaproic acids as end products of fermentation. The type strain of P. stomatis is W2278T (=DSM 17678T = CCUG 51858T); the G+C content of the DNA of this strain is 36 mol%. PMID:16585688

Downes, Julia; Wade, William G

2006-04-01

392

Rhizobium laguerreae sp. nov. nodulates Vicia faba on several continents.  

PubMed

Several fast-growing strains nodulating Vicia faba in Peru, Spain and Tunisia formed a cluster related to Rhizobium leguminosarum. The 16S rRNA gene sequences were identical to that of R. leguminosarum USDA 2370(T), whereas rpoB, recA and atpD gene sequences were phylogenetically distant, with sequence similarities of less than 96?%, 97?% and 94?%, respectively. DNA-DNA hybridization analysis showed a mean relatedness value of 43?% between strain FB206(T) and R. leguminosarum USDA 2370(T). Phenotypic characteristics of the novel strains also differed from those of the closest related species of the genus Rhizobium. Therefore, based on genotypic and phenotypic data obtained in this study, we propose to classify this group of strains nodulating Vicia faba as a novel species of the genus Rhizobium named Rhizobium laguerreae sp. nov. The type strain is FB206(T) (?=?LMG 27434(T)?=?CECT 8280(T)). PMID:24067731

Saïdi, Sabrine; Ramírez-Bahena, Martha-Helena; Santillana, Nery; Zúñiga, Doris; Álvarez-Martínez, Estela; Peix, Alvaro; Mhamdi, Ridha; Velázquez, Encarna

2014-01-01

393

Immobilization of glucosyltransferase from Erwinia sp. using two different techniques.  

PubMed

Two different techniques of glucosyltransferase immobilization were studied for the conversion of sucrose into isomaltulose. The optimum conditions for immobilization of Erwinia sp. glucosyltransferase onto Celite 545, determined using response surface methodology, was pH 4.0 and 170 U of glucosyltransferase/g of Celite 545. Using this conditions more than 60% conversion of sucrose into isomaltulose can be obtained. The immobilization of glucosyltransferase was also studied by its entrapment in microcapsules of low-methoxyl pectin and fat (butter and oleic acid). The non-lyophilized microcapsules of pectin, containing the enzyme and fat, showed higher glucosyltransferase activity, compared with lyophilized microcapsules containing enzyme plus fat, and also lyophilized microcapsules containing enzyme without fat addition. The non-lyophilized microcapsules of pectin containing the glucosyltransferase and fat, converted 30% of sucrose into isomaltulose in the first batch. However the conversion decreased to 5% at the 10th batch, indicating inactivation of the enzyme. PMID:22306307

Contesini, Fabiano Jares; Ibarguren, Carolina; Grosso, Carlos Raimundo Ferreira; Carvalho, Patrícia de Oliveira; Sato, Hélia Harumi

2012-04-15

394

Thermoelectric converter for SP-100 space reactor power system  

NASA Technical Reports Server (NTRS)

Conductively coupling the thermoelectric converter to the heat source and the radiator maximizes the utilization of the reactor and radiator temperatures and thereby minimizes the power system weight. This paper presents the design for the converter and the individual thermoelectric cells that are the building block modules for the converter. It also summarizes progress on the fabrication of initial cells and the results obtained from the preparation of a manufacturing plan. The design developed for the SP-100 system utilizes thermally conductive compliant pads that can absorb the displacement and distortion caused by the combinations of temperatures and thermal expansion coefficients. The converter and cell designs provided a 100 kWe system which met the system requirements. Initial cells were fabricated and tested.

Terrill, W. R.; Haley, V. F.

1986-01-01

395

[Microbiological diagnosis of microorganisms tentatively designated as "SP organisms"].  

PubMed

The authors describe the biochemical characteristics of two strains described as "SP organism". This microorganism incertae sedis resembles from the biochemical aspect (oxidase+, mannite-, dextrose+ with gas) the species Pasteurella aerogenes; contrary to the latter it does not break down urea and differs also as regards the morphology of colonies, which on blood agar are coarser; it also has a higher content of G + C in DNA than Pasteurella. It is a pathogen of rodents. The authors describe two isolates, the first is obviously an incidental finding from the faeces of a 5-year-old girl who was symptom-free, the second is from the contents of an abscess of a nutria. For comparison also biochemical characteristics of known aerogenic pasteurellae and Pasteurella-like strains are given. PMID:2632012

Aldová, E; Paucková, V; Vladík, P; Hausner, O; Sobotková, J; Lhotová, H

1989-12-01

396

Streptococcus halichoeri sp. nov., isolated from grey seals (Halichoerus grypus).  

PubMed

Phenotypic and phylogenetic studies were performed on six unidentified, Gram-positive, catalase-negative, chain-forming Streptococcus-like organisms recovered from grey seals. Biochemically the six strains were highly related to each other, but they did not appear to correspond to any recognized species of the genus Streptococcus. Comparative 16S rRNA gene sequencing studies confirmed that phylogenetically the strains were members of the genus Streptococcus, but sequence divergence values of greater than 3 % compared with reference streptococcal species demonstrated that the organisms from seals represent a novel species. SDS-PAGE analysis of whole-cell proteins confirmed the phenotypic distinctiveness of the seal organisms. Based on biochemical criteria and molecular chemical and genetic evidence, it is proposed that the unknown organism from seals be classified as a novel species, Streptococcus halichoeri sp. nov., the type strain of which is CCUG 48324T (= CIP 108195T). PMID:15388740

Lawson, Paul A; Foster, Geoffrey; Falsen, Enevold; Davison, Nick; Collins, Matthew D

2004-09-01

397

Two new compounds from gorgonian-associated fungus Aspergillus sp.  

PubMed

One new gamma-lactone derivative 5-hydroxy-3-isopropyl-4-methoxyfuranone (1) and one new lactam derivative dehydrated-marinamide (2), along with two known compounds marinamide (3) and marinamide methyl ester (4) were isolated from the fermentation broth of the marine gorgonian-associated fungus Aspergillus sp. SCSGAF0093. Their structures were elucidated on the basis of spectroscopic and spectrometric analysis. Compound 1 showed significant toxicity to brine shrimp (Artemia salina) with a median lethal concentration (LC50) of 1.25 microM, and 3 inhibited protein tyrosine phosphatase 1B (PTP1B) with a half maximal inhibitory concentration (IC50) of 23.3 microg/mL. PMID:24079168

Xu, Xin-Ya; Zhang, Xiao-Yong; He, Fei; Peng, Jiang; Nong, Xu-Hua; Qi, Shu-Hua

2013-08-01

398

Translational and transcriptional control of Sp1 against ischaemia through a hydrogen peroxide-activated internal ribosomal entry site pathway  

PubMed Central

The exact mechanism underlying increases in Sp1 and the physiological consequences thereafter remains unknown. In rat primary cortical neurons, oxygen-glucose deprivation (OGD) causes an increase in H2O2 as well as Sp1 in early ischaemia but apparently does not change mRNA level or Sp1 stability. We hereby identified a longer 5?-UTR in Sp1 mRNA that contains an internal ribosome entry site (IRES) that regulates rapid and efficient translation of existing mRNAs. By using polysomal fragmentation and bicistronic luciferase assays, we found that H2O2 activates IRES-dependent translation. Thus, H2O2 or tempol, a superoxide dismutase-mimetic, increases Sp1 levels in OGD-treated neurons. Further, early-expressed Sp1 binds to Sp1 promoter to cause a late rise in Sp1 in a feed-forward manner. Short hairpin RNA against Sp1 exacerbates OGD-induced apoptosis in primary neurons. While Sp1 levels increase in the cortex in a rat model of stroke, inhibition of Sp1 binding leads to enhanced apoptosis and cortical injury. These results demonstrate that neurons can use H2O2 as a signalling molecule to quickly induce Sp1 translation through an IRES-dependent translation pathway that, in cooperation with a late rise in Sp1 via feed-forward transcriptional activation, protects neurons against ischaemic damage. PMID:21441538

Yeh, Shiu Hwa; Yang, Wen Bin; Gean, Po Wu; Hsu, Chung Yi; Tseng, Joseph T.; Su, Tsung Ping; Chang, Wen Chang; Hung, Jan Jong

2011-01-01

399

Translational and transcriptional control of Sp1 against ischaemia through a hydrogen peroxide-activated internal ribosomal entry site pathway.  

PubMed

The exact mechanism underlying increases in Sp1 and the physiological consequences thereafter remains unknown. In rat primary cortical neurons, oxygen-glucose deprivation (OGD) causes an increase in H(2)O(2) as well as Sp1 in early ischaemia but apparently does not change mRNA level or Sp1 stability. We hereby identified a longer 5'-UTR in Sp1 mRNA that contains an internal ribosome entry site (IRES) that regulates rapid and efficient translation of existing mRNAs. By using polysomal fragmentation and bicistronic luciferase assays, we found that H(2)O(2) activates IRES-dependent translation. Thus, H(2)O(2) or tempol, a superoxide dismutase-mimetic, increases Sp1 levels in OGD-treated neurons. Further, early-expressed Sp1 binds to Sp1 promoter to cause a late rise in Sp1 in a feed-forward manner. Short hairpin RNA against Sp1 exacerbates OGD-induced apoptosis in primary neurons. While Sp1 levels increase in the cortex in a rat model of stroke, inhibition of Sp1 binding leads to enhanced apoptosis and cortical injury. These results demonstrate that neurons can use H(2)O(2) as a signalling molecule to quickly induce Sp1 translation through an IRES-dependent translation pathway that, in cooperation with a late rise in Sp1 via feed-forward transcriptional activation, protects neurons against ischaemic damage. PMID:21441538

Yeh, Shiu Hwa; Yang, Wen Bin; Gean, Po Wu; Hsu, Chung Yi; Tseng, Joseph T; Su, Tsung Ping; Chang, Wen Chang; Hung, Jan Jong

2011-07-01

400

Initial reactions of xanthone biodegradation by an Arthrobacter sp.  

PubMed Central

This study examined the catabolism of xanthone by an Arthrobacter sp. (strain GFB100) capable of growth on xanthone as its main source of carbon and energy. An early catabolic intermediate was 3,4-dihydroxyxanthone. This compound was isolated from the growth medium of a mutant strain of the Arthrobacter sp. which lacked the xanthone-inducible dihydroxyxanthone ring-fission dioxygenase of the wild-type strain. Cell extracts from wild-type xanthone-grown cells oxidized 3,4-dihydroxyxanthone to a yellow ring-fission metabolite. The same yellow compound accumulated in xanthone-grown cultures of a spontaneous mutant which lacked an active, xanthone-inducible, NADPH-linked ring-fission metabolite reductase. The yellow ring-fission metabolite appears to be 4-hydroxy-3-(2'-oxo-3-trans-butenoate)-coumarin, based on its nuclear magnetic resonance spectrum and mass spectral fragmentation pattern, indicating that ring cleavage of 3,4-dihydroxyxanthone was by an extra-diol (meta-fission) mechanism. Enzymatic analyses indicated that growth on xanthone induced a complete gentisate pathway: dioxygenase-catalyzed cleavage of gentisate to maleylpyruvate, isomerization of maleylpyruvate to fumarylpyruvate, and hydrolysis of fumarylpyruvate to fumarate and pyruvate. 4-Hydroxycoumarin was thought to be a likely pathway intermediate linking the early xanthone catabolic steps to the gentisate pathway, since 2-hydroxyacetophenone, a byproduct of 4-hydroxycoumarin hydrolysis, was formed when wild-type cells were cultured with xanthone. Chlorinated 2-hydroxyacetophenones were also obtained from specific chloro-substituted xanthones. PMID:3745120

Tomasek, P H; Crawford, R L

1986-01-01

401

Properties of an amylase from thermophilic Bacillus SP.  

PubMed

?-Amylase production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing soluble starch as a carbon source and supplemented with 0.05% whey protein and 0.2% peptone reached a maximum activity at 32 h, with levels of 37 U/mL. Studies on the amylase characterization revealed that the optimum temperature of this enzyme was 90°C. The enzyme was stable for 1 h at temperatures ranging from 40-50°C while at 90°C, 66% of its maximum activity was lost. However, in the presence of 5 mM CaCl2, the enzyme was stable at 90°C for 30 min and retained about 58% residual activity after 1 h. The optimum pH of the enzyme was found to be 8.5. After incubation of enzyme for 2 h at pH 9.5 and 11.0 was observed a decrease of about 6.3% and 16.5% of its original activity. At pH 6.0 the enzyme lost about 36% of its original activity. The enzyme was strongly inhibited by Co(2+), Cu(2+) and Ba(2+), but less affected by Mg(2+), Na(+) and K(+). In the presence of 2.0 M NaCl, 63% of amylase activity was retained after 2 h incubation at 45°C. The amylase exhibited more than 70% activity when incubated for 1 h at 50°C with sodium dodecyl sulphate. However, very little residual activity was obtained with sodium hypochlorite and with hydrogen peroxide the enzyme was completely inhibited. The compatibility of Bacillus sp SMIA-2 amylase with certain commercial detergents was shown to be good as the enzyme retained 86%, 85% and 75% of its activity after 20 min incubation at 50°C in the presence of the detergent brands Omo(®), Campeiro(®) and Tide(®), respectively. PMID:24031188

de Carvalho, Raquel Vieira; Côrrea, Thamy Lívia Ribeiro; da Silva, Júlia Caroline Matos; de Oliveira Mansur, Luciana Ribeiro Coutinho; Martins, Meire Lelis Leal

2008-01-01

402

Genomic sequence analysis and characterization of Sneathia amnii sp. nov  

PubMed Central

Background Bacteria of the genus Sneathia are emerging as potential pathogens of the female reproductive tract. Species of Sneathia, which were formerly grouped with Leptotrichia, can be part of the normal microbiota of the genitourinary tracts of men and women, but they are also associated with a variety of clinical conditions including bacterial vaginosis, preeclampsia, preterm labor, spontaneous abortion, post-partum bacteremia and other invasive infections. Sneathia species also exhibit a significant correlation with sexually transmitted diseases and cervical cancer. Because Sneathia species are fastidious and rarely cultured successfully in vitro; and the genomes of members of the genus had until now not been characterized, very little is known about the physiology or the virulence of these organisms. Results Here, we describe a novel species, Sneathia amnii sp. nov, which closely resembles bacteria previously designated "Leptotrichia amnionii". As part of the Vaginal Human Microbiome Project at VCU, a vaginal isolate of S. amnii sp. nov. was identified, successfully cultured and bacteriologically cloned. The biochemical characteristics and virulence properties of the organism were examined in vitro, and the genome of the organism was sequenced, annotated and analyzed. The analysis revealed a reduced circular genome of ~1.34 Mbp, containing ~1,282 protein-coding genes. Metabolic reconstruction of the bacterium reflected its biochemical phenotype, and several genes potentially associated with pathogenicity were identified. Conclusions Bacteria with complex growth requirements frequently remain poorly characterized and, as a consequence, their roles in health and disease are unclear. Elucidation of the physiology and identification of genes putatively involved in the metabolism and virulence of S. amnii may lead to a better understanding of the role of this potential pathogen in bacterial vaginosis, preterm birth, and other issues associated with vaginal and reproductive health. PMID:23281612

2012-01-01

403

Rhizobium halotolerans sp. nov., Isolated from chloroethylenes contaminated soil.  

PubMed

The strain designated as AB21(T) was isolated from chloroethylenes contaminated soil. Cells are gram-negative, aerobic, non-spore-forming, and motile rods. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Rhizobium, and was closely related to Rhizobium sullae IS 123(T) (97.4 %), Rhizobium yanglingense SH 22623(T) (97.2 %), Rhizobium gallicum R 602sp(T) (97.1 %), Rhizobium alamii GBV 016(T) (97.0 %), and Rhizobium monogolense USDA 1844(T) (97.0 %). It showed less than 97 % identity with the remaining Rhizobium species. This novel isolate grew optimally at 25-37 °C (optimum, 30 °C) and pH 6-9 (optimum, pH 8.0). It grew in the presence of 0-4 % (w/v) NaCl, tolerating a 4 % (w/v) NaCl. DNA-DNA hybridization experiment shows less than 53 % binding with closely related Rhizobium. Predominant quinone is ubiquinone (Q-10). The major fatty acids were summed feature 8 (composed of C(18:1) ?7c/C(18:1) ?6c), C(19:0) cyclo ?8c, and C(16:0). The G+C molar content is 62.5 mol%. Based on the polyphasic analysis, strain AB21(T) is referred to be a novel species of the genus Rhizobium for which the name Rhizobium halotolerans sp. nov. is proposed. The type strain is AB21(T) (=KEMC 224-056(T) = JCM 17536(T)). PMID:23377488

Diange, Eboa Adolf; Lee, Sang-Seob

2013-06-01

404

Mode of inhibition of respiratory syncytial virus by a plant flavonoid, SP-303.  

PubMed

A natural-product polyphenolic polymer of molecular weight 2,100 daltons designated SP-303, was found to have antiviral activity against respiratory syncytial virus (RSV) in the 2-10 microM range in plaque reduction assays and cytopathic-effect-inhibition assays. The material was also virucidal. The 50% effective concentration (EC50) for virucidal activity was 28 microM. Experiments were done to determine the mode(s) of RSV inhibition by SP-303. Interferon was not induced. SP-303 did not inhibit attachment at antiviral concentrations. However, the EC50 for inhibition of virus penetration by SP-303 was 0.48 +/- 0.19 microM. These data suggest that abolition of RSV penetration into host cells is one mechanism whereby SP-303 inhibits RSV replication. PMID:8508691

Barnard, D L; Huffman, J H; Meyerson, L R; Sidwell, R W

1993-01-01

405

SP600125 negatively regulates the mammalian target of rapamycin via ATF4-induced Redd1 expression.  

PubMed

SP600125 (SAPK Inhibitor II) is reported to function as a reversible ATP competitive inhibitor of c-Jun N-terminal kinase (JNK). In the present study, we show that SP600125 induces a dose-dependent decrease in mTOR activity, as assessed by reduced phosphorylation of the downstream targets S6K1 and S6, and a significant increase in the expression of Redd1. Knockdown of Redd1 expression by siRNA resulted in a recovery of decreased S6 phosphorylation by SP600125. Overexpression of ATF4 upregulated the expression of Redd1, while suppression of ATF4 expression by siRNA enhanced the level of S6 phosphorylation by downregulating the SP600125-induced increase in Redd1 expression. Together, these results indicate that SP600125 inhibits mTOR activity via an ATF4-induced increase in Redd1 expression. PMID:19059405

Jin, Hyeon-Ok; Seo, Sung-Keum; Woo, Sang-Hyeok; Kim, Eun-Sung; Lee, Hyung-Chahn; Yoo, Doo-Hyun; Choe, Tae-Boo; Hong, Seok-Il; Kim, Jong-Il; Park, In-Chul

2009-01-01

406

Prevalence of Giardia sp. in a beaver colony and the resulting environmental contamination.  

PubMed

The prevalence of Giardia sp. in a beaver (Castor canadensis) colony in Colorado was determined by the collection and analysis of fecal samples over a period of 14 mo. Environmental contamination was monitored through the use and analysis of water filter samples. Beaver shed cysts of Giardia sp. in their feces throughout the year with temporal variations in the prevalence, and became infected as kits and remained infected as juveniles and adults. Beaver served as amplification hosts for Giardia sp. and contaminated surface waters downstream from their dams in late spring and early fall. In slow moving waters the cysts of Giardia sp. settled rapidly. Muskrats (Ondatra zibethicus) were the only other species of wildlife shedding cysts of Giardia sp. on the study area. PMID:3682082

Monzingo, D L; Hibler, C P

1987-10-01

407

Dual catalysis. Merging photoredox with nickel catalysis: coupling of ?-carboxyl sp³-carbons with aryl halides.  

PubMed

Over the past 40 years, transition metal catalysis has enabled bond formation between aryl and olefinic (sp(2)) carbons in a selective and predictable manner with high functional group tolerance. Couplings involving alkyl (sp(3)) carbons have proven more challenging. Here, we demonstrate that the synergistic combination of photoredox catalysis and nickel catalysis provides an alternative cross-coupling paradigm, in which simple and readily available organic molecules can be systematically used as coupling partners. By using this photoredox-metal catalysis approach, we have achieved a direct decarboxylative sp(3)-sp(2) cross-coupling of amino acids, as well as ?-O- or phenyl-substituted carboxylic acids, with aryl halides. Moreover, this mode of catalysis can be applied to direct cross-coupling of C(sp³)-H in dimethylaniline with aryl halides via C-H functionalization. PMID:24903563

Zuo, Zhiwei; Ahneman, Derek T; Chu, Lingling; Terrett, Jack A; Doyle, Abigail G; MacMillan, David W C

2014-07-25

408

Expression of alternatively spliced isoforms of human Sp7 in osteoblast-like cells  

PubMed Central

Background Osteogenic and chondrocytic differentiation involves a cascade of coordinated transcription factor gene expression that regulates proliferation and matrix protein formation in a defined temporo-spatial manner. Bone morphogenetic protein-2 induces expression of the murine Osterix/Specificity protein-7 (Sp7) transcription factor that is required for osteoblast differentiation and bone formation. Regulation of its expression may prove useful for mediating skeletal repair. Results Sp7, the human homologue of the mouse Osterix gene, maps to 12q13.13, close to Sp1 and homeobox gene cluster-C. The first two exons of the 3-exon gene are alternatively spliced, encoding a 431-residue long protein isoform and an amino-terminus truncated 413-residue short protein isoform. The human Sp7 protein is a member of the Sp family having 78% identity with Sp1 in the three, Cys2-His2 type, DNA-binding zinc-fingers, but there is little homology elsewhere. The Sp7 mRNA was expressed in human foetal osteoblasts and craniofacial osteoblasts, chondrocytes and the osteosarcoma cell lines HOS and MG63, but was not detected in adult femoral osteoblasts. Generally, the expression of the short (or beta) protein isoform of Sp7 was much higher than the long (or alpha) protein isoform. No expression of either isoform was found in a panel of other cell types. However, in tissues, low levels of Sp7 were detected in testis, heart, brain, placenta, lung, pancreas, ovary and spleen. Conclusions Sp7 expression in humans is largely confined to osteoblasts and chondrocytes, both of which differentiate from the mesenchymal lineage. Of the two protein isoforms, the short isoform is most abundant. PMID:14604442

Milona, Maria-athina; Gough, Julie E; Edgar, Alasdair J

2003-01-01

409

The transcription factor Sp1 is responsible for aging-dependent altered nucleocytoplasmic trafficking.  

PubMed

Hyporesponsiveness to external signals, such as growth factors and apoptotic stimuli, is a cardinal feature of cellular senescence. We previously reported that an aging-dependent marked reduction in nucleocytoplasmic trafficking (NCT)-related genes could be responsible for this phenomenon. In searching for the mechanism, we identified the transcription factor, Sp1, as a common regulator of NCT genes, including various nucleoporins, importins, exportins, and Ran GTPase cycle-related genes. Sp1 knockdown led to a reduction of those genes in young human diploid fibroblast cells (HDF); Sp1 overexpression induced those genes in senescent cells. In addition, epidermal growth factor stimulation-induced p-ERK1/2 nuclear translocation and Elk-1 phosphorylation were severely impaired by Sp1 depletion in young HDFs; Sp1 overexpression restored the nuclear translocation of p-ERK1/2 in senescent HDFs. Furthermore, we observed that Sp1 protein levels were decreased in senescent cells, and H(2) O(2) treatment decreased Sp1 levels in a proteasome-dependent manner. In addition, O-GlcNAcylation of Sp1 was decreased in senescent cells as well as in H(2) O(2) -treated cells. Taken together, these results suggest that Sp1 could be a key regulator in the control of NCT genes and that reactive oxygen species-mediated alteration in Sp1 stability may be responsible for the generalized repression of those genes, leading to formation of the senescence-dependent functional nuclear barrier, resulting in subsequent hyporesponsiveness to external signals. PMID:23013401

Kim, Sung Y; Kang, Hyun T; Han, Jeong A; Park, Sang C

2012-12-01

410

Methanococcus vulcanius sp. nov., a novel hyperthermophilic methanogen isolated from East Pacific Rise, and identification of Methanococcus sp. DSM 4213T as Methanococcus fervens sp. nov.  

PubMed

An autotrophic, hyperthermophilic methanogen (M7T) was isolated from a deep-sea hydrothermal chimney sample collected on the East Pacific Rise at a depth of 2600 m. The coccoid-shaped cells are flagellated and exhibit a slight tumbling motility. The temperature range for growth at pH 6.5 was 49-89 degrees C, with optimum growth at 80 degrees C. The optimum pH for growth was 6.5, and the optimum NaCl concentration for growth was around 25 g l-1. The new isolate used H2 and CO2 as the only substrates for growth and methane production. Tungsten, selenium and yeast extract stimulated growth significantly. In the presence of CO2 and H2, the organism reduced elemental sulphur to hydrogen sulphide. Growth was inhibited by chloramphenicol and rifampicin, but not by ampicillin, kanamycin, penicillin and streptomycin. The G + C content of the genomic DNA was 31 mol%. As determined by 16S rDNA gene sequence analysis, this organism was closely related to Methanococcus jannaschii strain JAL-1T. However, despite the high percentage of similarity between their 16S rDNA sequences (97.1%), the DNA-DNA hybridization levels between these strains were less than 5%. On the basis of these observations and physiological traits, it is proposed that this organism should be placed in a new species, Methanococcus vulcanius. The type strain is M7T (= DSM 12094T). During the course of this study, the 16S rDNA sequence analysis placed Methanococcus sp. strain AG86T (= DSM 4213T) as a close relative of M. jannaschii strain JAL-1T. However, the weak level of DNA-DNA hybridization with this strain (< 10%) allowed the proposal that strain AG86T also constitutes a new species, Methanococcus fervens. PMID:10319479

Jeanthon, C; L'Haridon, S; Reysenbach, A L; Corre, E; Vernet, M; Messner, P; Sleytr, U B; Prieur, D

1999-04-01

411

Synthetic surfactant containing SP-B and SP-C mimics is superior to single-peptide formulations in rabbits with chemical acute lung injury  

PubMed Central

Background. Chemical spills are on the rise and inhalation of toxic chemicals may induce chemical acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Although the pathophysiology of ALI/ARDS is well understood, the absence of specific antidotes has limited the effectiveness of therapeutic interventions. Objectives. Surfactant inactivation and formation of free radicals are important pathways in (chemical) ALI. We tested the potential of lipid mixtures with advanced surfactant protein B and C (SP-B and C) mimics to improve oxygenation and lung compliance in rabbits with lavage- and chemical-induced ALI/ARDS. Methods. Ventilated young adult rabbits underwent repeated saline lung lavages or underwent intratracheal instillation of hydrochloric acid to induce ALI/ARDS. After establishment of respiratory failure rabbits were treated with a single intratracheal dose of 100 mg/kg of synthetic surfactant composed of 3% Super Mini-B (S-MB), a SP-B mimic, and/or SP-C33 UCLA, a SP-C mimic, in a lipid mixture (DPPC:POPC:POPG 5:3:2 by weight), the clinical surfactant Infasurf®, a bovine lung lavage extract with SP-B and C, or synthetic lipids alone. End-points consisted of arterial oxygenation, dynamic lung compliance, and protein and lipid content in bronchoalveolar lavage fluid. Potential mechanism of surfactant action for S-MB and SP-C33 UCLA were investigated with captive bubble surfactometry (CBS) assays. Results. All three surfactant peptide/lipid mixtures and Infasurf equally lowered the minimum surface tension on CBS, and also improved oxygenation and lung compliance. In both animal models, the two-peptide synthetic surfactant with S-MB and SP-C33 UCLA led to better arterial oxygenation and lung compliance than single peptide synthetic surfactants and Infasurf. Synthetic surfactants and Infasurf improved lung function further in lavage- than in chemical-induced respiratory failure, with the difference probably due to greater capillary-alveolar protein leakage and surfactant dysfunction after HCl instillation than following lung lavage. At the end of the duration of the experiments, synthetic surfactants provided more clinical stability in ALI/ARDS than Infasurf, and the protein content of bronchoalveolar lavage fluid was lowest for the two-peptide synthetic surfactant with S-MB and SP-C33 UCLA. Conclusion. Advanced synthetic surfactant with robust SP-B and SP-C mimics is better equipped to tackle surfactant inactivation in chemical ALI than synthetic surfactant with only a single surfactant peptide or animal-derived surfactant. PMID:24883253

Hernandez-Juviel, Jose M.; Gordon, Larry M.; Waring, Alan J.

2014-01-01

412

The explicit representation for the angular flux solution in the simplified P{sub N} (SP{sub N}) theory  

SciTech Connect

The current SP{sub n} theory formulation, via either the asymptotic method or the variational method, does not provide an explicit and calculable representation for the corresponding angular flux solution. It is therefore not possible to reconstruct from the SP{sub n} solution the corresponding angular flux solution, or to extract from a reference transport solution the corresponding SP{sub n} solution. This makes it impossible to calculate the necessary surface discontinuity factors to force consistency between the SP{sub n} solution and the higher level transport solution. Without discontinuity factors, the superiority of SP{sub n} over diffusion could be significantly degraded in practical applications. In this paper we present a different SP{sub n} formulation that provides the explicit angular flux solution such that the physical picture for the SP{sub n} approximation is transparent and the SP{sub n} discontinuity factors can be calculated. (authors)

Chao, Y. A. [Apartment 101, Building 2, 788 Hong Xu Road, Shanghai 201103 (China); Yamamoto, A. [Nagoya Univ., Furo-cho, Chikusa-ku, Nagoya, 464-8603 (Japan)

2012-07-01

413

Draft Genome Sequence of the Agar-Degrading Bacterium Catenovulum sp. Strain DS-2, Isolated from Intestines of Haliotis diversicolor  

PubMed Central

Catenovulum sp. strain DS-2, isolated from intestines of Haliotis diversicolor, is able to degrade agar and produce agaro-oligosaccharides. Here, we report the draft genome sequence of Catenovulum sp. strain DS-2. PMID:24604650

Shan, Dapeng; Li, Xu; Gu, Zheng; Wei, Guangshan; Gao, Zheng

2014-01-01

414

Mesorhizobium gobiense sp. nov. and Mesorhizobium tarimense sp. nov., isolated from wild legumes growing in desert soils of Xinjiang, China.  

PubMed

Twenty-four Mesorhizobium strains were isolated from desert soils in the Xinjiang region of China and were characterized by a polyphasic approach. These strains grouped into three clusters in IGS-RFLP, SDS-PAGE analysis of whole-cell proteins and BOX-PCR analysis, corresponding to genomic species V, VI and VII as found in a previous study. The results were supported by sequencing analyses of rrs, IGS, atpD and recA genes. Genospecies VII was most related to Mesorhizobium septentrionale, while genospecies V and VI were both most closely related to Mesorhizobium tianshanense, but were distinct from each other and from M. tianshanense. The DNA-DNA hybridization value between the representative strain CCBAU 83284 (genospecies VII) and the type strain of M. septentrionale was 90.1 %. Genospecies VII was thus defined as M. septentrionale. The DNA-DNA relatedness value for representative strains of genospecies V or VI with the related reference strains of recognized species were always lower than 60 %. Low values of DNA-DNA hybridization (32.79 %) between representative strains of genospecies V (CCBAU 83330(T)) and of VI (CCBAU 83306(T)) were also observed. Based upon these results, two novel species are proposed: Mesorhizobium gobiense sp. nov. represented by genospecies V (type strain, CCBAU 83330(T)=LMG 23949(T)=HAMBI 2974(T)) and Mesorhizobium tarimense sp. nov. represented by genospecies VI (type strain, CCBAU 83306(T)=LMG 24338(T)=HAMBI 2973(T)). Strain CCBAU 83278 grouped as the most peripheral member with genospecies VI in SDS-PAGE of whole-cell proteins and BOX-PCR analysis and in the phylogenetic tree of 16S-23S rRNA intergenic spacer (IGS) sequences. The results of analyses of rrs, atpD and recA gene sequences, as well as those of DNA-DNA hybridization studies, strongly supported the suggestion that this strain belonged to a species quite different from genospecies V and VI and from any other recognized species of the genus Mesorhizobium. As only one strain has been isolated to date, strain CCBAU 83278 was not proposed as a novel species in this study. Mesorhizobium gobiense sp. nov. and Mesorhizobium tarimense sp. nov. could be differentiated from each other as well as from recognized species of the genus Mesorhizobium on the basis of phenotypic characteristics. The symbiotic loci (nodC and nifH) of the two novel species formed two phylogenetic branches related to Mesorhizobium loti and M. tianshanense. The type strains of the two novel species were able to nodulate Glycyrrhiza uralensis, Lotus corniculatus, Oxytropis glabra and Robinia pseudoacacia but not Astragalus membranaceus, Leucaena leucocephala, Phaseolus vulgaris, Pisum sativum or Medicago sativa. PMID:18984702

Han, Tian Xu; Han, Li Li; Wu, Li Juan; Chen, Wen Feng; Sui, Xin Hua; Gu, Jin Gang; Wang, En Tao; Chen, Wen Xin

2008-11-01

415

Description of Two Species of Early Branching Dinoflagellates, Psammosa pacifica n. g., n. sp. and P. atlantica n. sp  

PubMed Central

In alveolate evolution, dinoflagellates have developed many unique features, including the cell that has epicone and hypocone, the undulating transverse flagellum. However, it remains unclear how these features evolved. The early branching dinoflagellates so far investigated such as Hematodinium, Amoebophrya and Oxyrrhis marina differ in many ways from of core dinoflagellates, or dinokaryotes. Except those handful of well studied taxa, the vast majority of early branching dinoflagellates are known only by environmental sequences, and remain enigmatic. In this study we describe two new species of the early branching dinoflagellates, Psammosa pacifica n. g., n. sp. and P. atlantica n. sp. from marine intertidal sandy beach. Molecular phylogeny of the small subunit (SSU) ribosomal RNA and Hsp90 gene places Psammosa spp. as an early branch among the dinoflagellates. Morphologically (1) they lack the typical dinoflagellate epicone–hypocone structure, and (2) undulation in either flagella. Instead they display a mosaïc of dinokaryotes traits, i.e. (3) presence of bi-partite trychocysts; Oxyrrhis marina–like traits, i.e. (4) presence of flagellar hairs, (5) presence of two-dimensional cobweb scales ornamenting both flagella (6) transversal cell division; a trait shared with some syndineansand Parvilucifera spp. i.e. (7) a nucleus with a conspicuous nucleolus and condensed chromatin distributed beneath the nuclear envelope; as well as Perkinsus marinus -like features i.e. (8) separate ventral grooves where flagella emerge and (9) lacking dinoflagellate-type undulating flagellum. Notably Psammosa retains an apical complex structure, which is shared between perkinsids, colpodellids, chromerids and apicomplexans, but is not found in dinokaryotic dinoflagellates. PMID:22719825

Okamoto, Noriko; Horak, Ales; Keeling, Patrick J.

2012-01-01

416

Thermococcus gorgonarius sp. nov. and Thermococcus pacificus sp. nov.: heterotrophic extremely thermophilic archaea from New Zealand submarine hot vents.  

PubMed

Two extremely thermophilic archaea, designated W-12 and P-4, were isolated from a geothermal vent in the tidal zone of Whale Island, New Zealand, and from geothermally heated bottom deposits of the Bay of Plenty, New Zealand, respectively. Cells of isolate W-12 are irregular cocci, 0.3-1.2 microns in diameter, motile with polar flagella. The cell envelope consists of one layer of subunits with a major protein of M(r) 75,000. Cells produce protrusions of different kinds: prostheca-like, chains of bubbles, or network of fimbriae. Cells of isolate P-4 are regular cocci, 0.7-1.0 micron in diameter, motile with polar flagella. The cell envelope consists of two layers of subunits; its major protein has an M(r) of 56,000. Both organisms are obligate anaerobes, fermenting peptides in the case of strain W-12, or peptides and starch in the case of P-4. Elemental sulfur is required for growth and is reduced to hydrogen sulfide. The optimal growth temperature of the new isolates is in the range 80-88 degrees C. The optimal growth pH is 6.5-7.2. The G + C content of the DNA of strain W-12 is 50.6 mol%, and of strain P-4 is 53.3 mol%. Based on physiological characteristics, 165 rDNA sequence comparison and DNA base composition, the new isolates were considered to be members of the genus Thermococcus. The low level of DNA-DNA hybridization with the type strains of other Thermococcus species confirms the novel species status of the new isolates. The new isolates are described as Thermococcus gorgonarius sp. nov., with type strain W-12 (= DSM 10395T), and Thermococcus pacificus sp. nov., with type strain P-4 (= DSM 10394T). PMID:9542072

Miroshnichenko, M L; Gongadze, G M; Rainey, F A; Kostyukova, A S; Lysenko, A M; Chernyh, N A; Bonch-Osmolovskaya, E A

1998-01-01

417

Thermotoga profunda sp. nov. and Thermotoga caldifontis sp. nov., anaerobic thermophilic bacteria isolated from terrestrial hot springs.  

PubMed

Two thermophilic, strictly anaerobic, Gram-negative bacteria, designated strains AZM34c06(T) and AZM44c09(T), were isolated from terrestrial hot springs in Japan. The optimum growth conditions for strain AZM34c06(T) were 60 °C, pH 7.4 and 0% additional NaCl, and those for strain AZM44c09(T) were 70 °C, pH 7.4 and 0% additional NaCl. Complete genome sequencing was performed for both strains, revealing genome sizes of 2.19 Mbp (AZM34c06(T)) and 2.01 Mbp (AZM44c09(T)). Phylogenetic analyses based on 16S rRNA gene sequences and the concatenated predicted amino acid sequences of 33 ribosomal proteins showed that both strains belonged to the genus Thermotoga. The closest relatives of strains AZM34c06(T) and AZM44c09(T) were the type strains of Thermotoga lettingae (96.0% similarity based on the 16S rRNA gene and 84.1% similarity based on ribosomal proteins) and Thermotoga hypogea (98.6 and 92.7% similarity), respectively. Using blast, the average nucleotide identity was 70.4-70.5% when comparing strain AZM34c06(T) and T. lettingae TMO(T) and 76.6% when comparing strain AZM44c09(T) and T. hypogea NBRC 106472(T). Both values are far below the 95% threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus Thermotoga within two novel species, Thermotoga profunda sp. nov. (type strain AZM34c06(T)?=?NBRC 106115(T)?=?DSM 23275(T)) and Thermotoga caldifontis sp. nov. (type strain AZM44c09(T)?=?NBRC 106116(T)?=?DSM 23272(T)). PMID:24676729

Mori, Koji; Yamazoe, Atsushi; Hosoyama, Akira; Ohji, Shoko; Fujita, Nobuyuki; Ishibashi, Jun-ichiro; Kimura, Hiroyuki; Suzuki, Ken-ichiro

2014-06-01

418

The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice  

PubMed Central

Background Six independent Gram-negative, facultatively anaerobic, non-spore-forming, nitrogen-fixing rod-shaped isolates were obtained from the root endosphere of rice grown at the International Rice Research Institute (IRRI) and investigated in a polyphasic taxonomic study. Results The strains produced fatty acid patterns typical for members of the family Enterobacteriaceae. Comparative sequence analyses of the 16S rRNA as well as rpoB genes allocated the strains to two well-defined groups within the genus Enterobacter, family Enterobacteriaceae. The analyses indicated Enterobacter radicincitans, Enterobacter arachidis and Enterobacter oryzae to be the closest related species. An RpoB (translated) protein comparison supported the placement in the genus Enterobacter and the relatedness of our isolates to the aforementioned species. Genomic DNA:DNA hybridization analyses and biochemical analyses provided further evidence that the novel strains belong to two new species within the genus Enterobacter. The two species can be differentiated from each other and from existing enteric species by acid production from L-rhamnose and D-melibiose, decarboxylation of ornithine and utilization of D-alanine, D-raffinose L-proline and L-aspartic acid, among other characteristics. Members of both species revealed capacities to colonise rice roots, including plant-growth-promoting capabilities such as an active supply of fixed nitrogen to the plant and solubilisation of inorganic phosphorus, next to traits allowing adaptation to the plant. Conclusions Two novel proposed enterobacterial species, denominated Enterobacter oryziphilus sp. nov. (type strain REICA_142T=LMG 26429T=NCCB 100393T) and Enterobacter oryzendophyticus sp. nov. (type strain REICA_082T=LMG 26432T =NCCB 100390T) were isolated from rice roots. Both species are capable of promoting rice growth by supplying nitrogen and phosphorus. PMID:23865888

2013-01-01

419

Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes.  

PubMed

Taxonomic studies were performed on seven strains isolated from microbial mats in Antarctic lakes of the McMurdo Dry Valleys. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains are related to the genus Flavobacterium; sequence similarity values with their nearest phylogenetic neighbours ranged from 97.0 to 98.7 %. Results of DNA-DNA hybridization and comparison of repetitive extragenic palindromic DNA-PCR fingerprinting patterns revealed that these strains are members of two distinct species. Genotypic results, together with phenotypic characteristics, allowed these species to be differentiated from related recognized Flavobacterium species. The isolates are psychrophilic, Gram-negative, chemoheterotrophic, rod-shaped cells. Their whole-cell fatty acid profiles are similar and include C(15 : 0), C(15 : 0) anteiso, C(15 : 0) iso, C(15 : 1)omega6c, C(16 : 0) iso, C(16 : 0) iso 3-OH, C(16 : 1) iso and summed feature 3 (which comprises C(15 : 0) iso 2-OH and/or C(16 : 1)omega7c) as the major fatty acid components. On the basis of these results, two novel species are proposed: Flavobacterium fryxellicola sp. nov., consisting of three strains with LMG 22022(T) (=CIP 108325(T)) as the type strain; and Flavobacterium psychrolimnae sp. nov., consisting of four strains with LMG 22018(T) (=CIP 108326(T)) as the type strain. DNA G+C contents of F. fryxellicola and F. psychrolimnae are 35.2-35.9 and 33.8-34.5 mol%, respectively. PMID:15774660

Van Trappen, Stefanie; Vandecandelaere, Ilse; Mergaert, Joris; Swings, Jean

2005-03-01

420

Streptomyces chiangmaiensis sp. nov. and Streptomyces lannensis sp. nov., isolated from the South-East Asian stingless bee (Tetragonilla collina).  

PubMed

Two novel actinomycetes, strains TA4-1(T) and TA4-8(T,) were isolated from the South-East Asian stingless bee (Tetragonilla collina Smith 1857), collected from Chiang Mai Province, Thailand. The morphological and chemotaxonomic properties of strains TA4-1(T) and TA4-8(T) were consistent with the genus Streptomyces, i.e. the formation of aerial mycelia bearing spiral spore chains, the presence of the ll-isomer of diaminopimelic acid in cell walls, iso- and anteiso-branched fatty acids with carbon chain lengths 14-17 atoms as the major fatty acids and MK-9(H8) as the predominant menaquinone plus minor amounts of MK-9(H6) and MK-9(H10). Analysis of 16S rRNA gene sequences showed that strains TA4-1(T) and TA4-8(T) exhibited 98.8 and 98.1% sequence similarity, respectively, with Streptomyces chromofuscus NRRL B-12175(T) and 98.9% sequence similarity with each other. This study suggested that strains TA4-1(T) and TA4-8(T) were distinct from previously described species of the genus Streptomyces. In addition, the low degrees of DNA-DNA relatedness between the isolates and S. chromofuscus JCM 4354(T) warranted assigning strains TA4-1(T) and TA4-8(T) to two novel species. The names Streptomyces chiangmaiensis sp. nov. (type strain TA4-1(T) ?=?JCM 16577(T) ?=?TISTR 1981(T)) and Streptomyces lannensis sp. nov. (type strain TA4-8(T) ?=?JCM 16578(T) ?=?TISTR 1982(T)) are proposed. The species names indicate the geographical locations where the stingless bees reside. PMID:23002051

Promnuan, Yaowanoot; Kudo, Takuji; Ohkuma, Moriya; Chantawannakul, Panuwan

2013-05-01

421

Methylobacterium trifolii sp. nov. and Methylobacterium thuringiense sp. nov., methanol-utilizing, pink-pigmented bacteria isolated from leaf surfaces.  

PubMed

Three pink-pigmented, aerobic, Gram-stain-negative, rod-shaped and facultatively methylotrophic strains were isolated from the phyllosphere of Trifolium repens and Cerastium holosteoides. 16S rRNA gene sequence analysis support the affiliation of all strains to the genus Methylobacterium. The closest relatives of strains C34(T) and T5 were Methylobacterium gnaphalii 23e(T) (98.0 and 98.5 % sequence similarity, respectively) and Methylobacterium organophilum JCM 2833(T) (97.0 and 97.2 %, respectively). Strain TA73(T) showed the highest sequence similarities to Methylobacterium marchantiae JT1(T) and Methylobacterium bullatum F3.2(T) (both 97.9 %), followed by Methylobacterium phyllosphaerae CBMB27(T) and Methylobacterium brachiatum DSM 19569(T) (both 97.8 %), Methylobacterium cerastii C15(T) and Methylobacterium radiotolerans JCM 2831(T) (both 97.7 %). The major components in the fatty acid profiles were C18 : 1?7c, C16 : 0 and one unknown fatty acid for strain TA73(T) and C18 : 1?7c, C16 : 1?7c/iso-C15 : 0 2-OH, C18 : 0 and C16 : 0 for strains C34(T) and T5. Physiological and biochemical analysis, including DNA-DNA hybridization, revealed clear differences between the investigated strains and their closest phylogenetic neighbours. DNA-DNA hybridization studies also showed high similarities between strains C34(T) and T5 (59.6-100 %). Therefore, the isolates represent two novel species within the genus Methylobacterium, for which the names Methylobacterium trifolii sp. nov. (type strain TA73(T) = LMG 25778(T) = CCM 7786(T)) and Methylobacterium thuringiense sp. nov. (type strain C34(T) = LMG 25777(T) = CCM 7787(T)) are proposed. PMID:23291886

Wellner, S; Lodders, N; Glaeser, S P; Kämpfer, P

2013-07-01

422

Ni-catalyzed carboxylation of C(sp2)- and C(sp3)-O bonds with CO2.  

PubMed

In recent years a significant progress has been made for the carboxylation of aryl and benzyl halides with CO2, becoming convenient alternatives to the use of stoichiometric amounts of well-defined metal species. Still, however, most of these processes require the use of pyrophoric and air-sensitive reagents and the current methods are mostly restricted to organic halides. Therefore, the discovery of a mild, operationally simple alternate carboxylation that occurs with a wide substrate scope employing readily available coupling partners will be highly desirable. Herein, we report a new protocol that deals with the development of a synergistic activation of CO2 and a rather challenging activation of inert C(sp(2))-O and C(sp(3))-O bonds derived from simple and cheap alcohols, a previously unrecognized opportunity in this field. This unprecedented carboxylation event is characterized by its simplicity, mild reaction conditions, remarkable selectivity pattern and an excellent chemoselectivity profile using air-, moisture-insensitive and easy-to-handle nickel precatalysts. Our results render our method a powerful alternative, practicality and novelty aside, to commonly used organic halides as counterparts in carboxylation protocols. Furthermore, this study shows, for the first time, that traceless directing groups allow for the reductive coupling of substrates without extended ?-systems, a typical requisite in many C-O bond-cleavage reactions. Taking into consideration the limited knowledge in catalytic carboxylative reductive events, and the prospective impact of providing a new tool for accessing valuable carboxylic acids, we believe this work opens up new vistas and allows new tactics in reductive coupling events. PMID:24377699

Correa, Arkaitz; León, Thierry; Martin, Ruben

2014-01-22

423

Ferrimicrobium acidiphilum gen. nov., sp. nov. and Ferrithrix thermotolerans gen. nov., sp. nov.: heterotrophic, iron-oxidizing, extremely acidophilic actinobacteria.  

PubMed

Two novel extremely acidophilic, iron-oxidizing actinobacteria were isolated, one from a mine site in North Wales, UK (isolate T23(T)), and the other from a geothermal site in Yellowstone National Park, Wyoming, USA (Y005(T)). These new actinobacteria belong to the subclass Acidimicrobidae, and in contrast to the only other classified member of the subclass (Acidimicrobium ferrooxidans), both isolates were obligate heterotrophs. The mine site isolate was mesophilic and grew as small rods, while the Yellowstone isolate was a moderate thermophile and grew as long filaments, forming macroscopic flocs in liquid media. Both isolates accelerated the oxidative dissolution of pyrite in yeast extract-amended cultures, but neither was able to oxidize reduced forms of sulfur. Ferrous iron oxidation enhanced growth yields of the novel mesophilic actinobacterium T23(T), though this was not confirmed for the Yellowstone isolate. Both isolates catalysed the dissimilatory reduction of ferric iron, using glycerol as electron donor, in oxygen-free medium. Based on comparative analyses of base compositions of their chromosomal DNA and of their 16S rRNA gene sequences, the isolates are both distinct from each other and from Acidimicrobium ferrooxidans, and are representatives of two novel genera. The names Ferrimicrobium acidiphilum gen. nov., sp. nov. and Ferrithrix thermotolerans gen. nov., sp. nov. are proposed for the mesophilic and moderately thermophilic isolates, respectively, with the respective type strains T23(T) (=DSM 19497(T)=ATCC BAA-1647(T)) and Y005(T) (=DSM 19514(T)=ATCC BAA-1645(T)). PMID:19406797

Johnson, D Barrie; Bacelar-Nicolau, Paula; Okibe, Naoko; Thomas, Angharad; Hallberg, Kevin B

2009-05-01

424

A novel gene encoding a 54 kDa polypeptide is essential for butane utilization by Pseudomonas sp. IMT37  

Microsoft Academic Search

Twenty-three propane- and butane-utilizing bacteria were isolated from soil samples collected from oilfields. Three of them have been identified as Rhodococcus sp. IMT35, Pseudomonas sp. IMT37 and Pseudomonas sp. IMT40. SDS-PAGE analysis of the membrane of Rhodococcus sp. IMT35 revealed the presence of at least four polypeptides induced by propane. Polyclonal antibody raised against a 58 kDa polypeptide from Rhodococcus

R. S. Padda; K. K. Pandey; S. Kaul; V. D. Nair; R. K. Jain; S. K. Basus; T. Chakrabarti

425

Role of microzooplankton during a Phaeocystis sp. bloom in the Oosterschelde (SW Netherlands)  

NASA Astrophysics Data System (ADS)

The impact of microzooplankton on the plankton community was assessed during a bloom of Phaeocystis sp. in the Oosterschelde basin (SW Netherlands). Chlorophyll a concentration and Phaeocystis sp. abundance reached maxima values in the middle of the sampling period (~ 27 mg chl a m- 3 and 3.4 × 1010 cells m- 3, respectively). The bloom collapse coincided with the last sampling day, when accentuated decreases in chlorophyll a concentrations (~ 11 mg chl a m- 3) and Phaeocystis sp. cells numbers (~ 1.3 × 1010 cells m- 3) were recorded. Microzooplankton organisms were significant consumers of both phytoplankton and heterotrophic plankton. Although Phaeocystis sp. was the most consumed organism (336 ± 71 mg C m- 3 d- 1), microzooplankton impact on its standing stock was lower than on the stocks of other less abundant organisms. This impact was also lower during the peak of the bloom, when colonial forms of Phaeocystis sp. presumably predominated, than during the bloom collapse, when free-living cells were supposedly more abundant. The impact of microzooplankton on heterotrophic organisms was higher than on phytoplankton, and increased when the bloom collapsed. Picoheterotrophs experienced the highest impact on their standing stock (~ 75% d- 1) and production (~ 90% d- 1). These results demonstrate that during a Phaeocystis sp. bloom, the microbial food web was responsible for channelling a significant fraction of plankton biomass, either from direct consumption of Phaeocystis sp. cells or through consumption of heterotrophs, which would have been favoured by the high quantities of organic matter released during the bloom collapse.

Teixeira, I. G.; Crespo, B. G.; Nielsen, T. G.; Figueiras, F. G.

2012-06-01

426

Azolla filiculoides Nitrogenase Activity Decrease Induced by Inoculation with Chlamydomonas sp.  

PubMed

Experiments were conducted to determine the influence of Chlamydomonas sp. on nitrogen fixation (C(2)H(2) --> C(2)H(4)) in Azolla filiculoides and on the nitrogen fixation and growth of free-living Anabaena azollae 2B organisms. Inoculation of azolla medium with Chlamydomonas sp. was associated with decreased nitrogenase activity in A. filiculoides and with increases in the density of a fungal population identified as Acremonium sp. Subsequent inoculation of azolla medium with this fungus was also accompanied by a significant decrease in nitrogenase activity of A. filiculoides. However, the extent of depression of nitrogenase activity was significantly higher when azolla medium was inoculated with Chlamydomonas sp. than when it was inoculated with Acremonium sp. Inoculation of nitrogen-free Stanier medium with either Acremonium sp. or Chlamydomonas sp. did not adversely affect the growth or nitrogenase activity of free-living A. azollae. Decreased nitrogenase activity in A. filiculoides is apparently related to the adverse influence of the green alga and the fungus on the macrosymbiont. The mechanisms that might be involved are discussed. PMID:16347211

Habte, M

1986-11-01

427

Azolla filiculoides Nitrogenase Activity Decrease Induced by Inoculation with Chlamydomonas sp. †  

PubMed Central

Experiments were conducted to determine the influence of Chlamydomonas sp. on nitrogen fixation (C2H2 ? C2H4) in Azolla filiculoides and on the nitrogen fixation and growth of free-living Anabaena azollae 2B organisms. Inoculation of azolla medium with Chlamydomonas sp. was associated with decreased nitrogenase activity in A. filiculoides and with increases in the density of a fungal population identified as Acremonium sp. Subsequent inoculation of azolla medium with this fungus was also accompanied by a significant decrease in nitrogenase activity of A. filiculoides. However, the extent of depression of nitrogenase activity was significantly higher when azolla medium was inoculated with Chlamydomonas sp. than when it was inoculated with Acremonium sp. Inoculation of nitrogen-free Stanier medium with either Acremonium sp. or Chlamydomonas sp. did not adversely affect the growth or nitrogenase activity of free-living A. azollae. Decreased nitrogenase activity in A. filiculoides is apparently related to the adverse influence of the green alga and the fungus on the macrosymbiont. The mechanisms that might be involved are discussed. PMID:16347211

Habte, Mitiku

1986-01-01

428

Genome sequencing of multidrug resistant novel Clostridium sp. BL8 reveals its potential for pathogenicity  

PubMed Central

Background The human gut microbiome is important for maintaining the health status of the host. Clostridia are key members of the human gut microbiome, carrying out several important functions in the gut environment. Hence understanding the role of different Clostridium species isolated from human gut is essential. The present study was aimed at investigating the role of novel Clostridium sp. isolate BL8 in human gut using genome sequencing as a tool. Findings The genome analysis of Clostridium sp. BL8 showed the presence of several adaptive features like bile resistance, presence of sensory and regulatory systems, presence of oxidative stress managing systems and presence of membrane transport systems. The genome of Clostridium sp. BL8 consists of a wide variety of virulence factors like phospholipase C (alpha toxin), hemolysin, aureolysin and exfoliative toxin A, as well as adhesion factors, proteases, Type IV secretion system and antibiotic resistance genes. In vitro antibiotic sensitivity testing showed that Clostridium sp. BL8 was resistant to 11 different tested antibiotics belonging to 6 different classes. The cell cytotoxicity assay confirmed the cytotoxic effect of Clostridium sp. BL8 cells, which killed 40% of the Vero cells after 4 hrs of incubation. Conclusions Clostridium sp. BL8 has adapted for survival in human gut environment, with presence of different adaptive features. The presence of several virulence factors and cell cytotoxic activity indicate that Clostridium sp. BL8 has a potential to cause infections in humans, however further in vivo studies are necessary to ascertain this fact. PMID:25076986

2014-01-01

429

Negative Regulation of DsbA-L Gene Expression by the Transcription Factor Sp1.  

PubMed

Disulfide-bond A oxidoreductase-like protein (DsbA-L) possesses beneficial effects such as promoting adiponectin multimerization and stability, increasing insulin sensitivity, and enhancing energy metabolism. The expression level of DsbA-L is negatively correlated with obesity in mice and humans, but the underlying mechanisms remain unknown. To address this question, we generated reporter gene constructs containing the promoter sequence of the mouse DsbA-L gene. Deletion analysis showed that the proximal promoter of mouse DsbA-L is located between -186 and -34 bp relative to the transcription start site. In silico analysis identified a putative Sp1 transcription factor binding site in the first intron of the DsbA-L gene. Electrophoretic mobility shift assay and chromatin immunoprecipitation analysis indicated that Sp1 bound to this intron region in vitro and in intact cells. Overexpression of Sp1 or suppressing Sp1 expression by siRNA reduced or increased DsbA-L promoter activity, respectively. The binding activity of Sp1 was gradually decreased during 3T3-L1 cell differentiation and was significantly increased in adipose tissues of obese mice. Our results identify Sp1 as an inhibitor of DsbA-L gene transcription, and the Sp1-mediated inhibition of DsbA-L gene expression may provide a mechanism underlying obesity-induced adiponectin downregulation and insulin resistance. PMID:25024375

Fang, Qichen; Yang, Wenjing; Li, Huating; Hu, Wenxiu; Chen, Lihui; Jiang, Shan; Dong, Kun; Song, Qianqian; Wang, Chen; Chen, Shuo; Liu, Feng; Jia, Weiping

2014-12-01

430

Freshwater abiotic components' impact on the viability of fish lice, Argulus sp., in Guangdong province, China.  

PubMed

Parasite intensity dynamics can be strongly affected by ecosystem abiotic components. We conducted this study to investigate how the intensity of Argulus sp. is influenced by physicochemical factors in three water bodies - river, fish farm, and reservoir - and to find the occurrence of Argulus sp. under different conditions. Argulus sp. specimens were collected using different methods - gill-netting 30 × 1.5 m, 40 mm mesh monofilament net and set from a small boat, beach seining and plankton net - from July 2010 until Jun 2011 at Guangdong province, China. Main water parameters taken for the assessment consisting of temperature, pH, DO, biochemical oxygen demand of 5 days (BOD5), chemical oxygen demand (COD), NH (4) (+) , total nitrogen (TN) and total phosphorus (TP). The results show that the ratio of female to male Argulus sp. during the studied period was 2.3:1. The presence and intensity of Argulus sp. were temperature-dependent and variable; pH values were lower than standers with high intensity of Argulus sp. Argulus sp. has the ability to live in low dissolved oxygen (DO) concentration. Relatively high concentrations of BOD5, COD, NH (4) (+) , TN and TP were detected with related variable intensity of Argulus sp., which is supposed to be a freshwater fish ectoparasite found in polluted ecosystems. It is concluded that the fish ectoparasite Argulus sp. have a strong potency of genetic adaptation impacted by environmental factors. However, this needs further study in order to gain insight into the question of the likelihood of adaptation to abiotic factors' variation. PMID:22322389

Alsarakibi, Muhamd; Wadeh, Hicham; Li, Guoqing

2012-07-01

431

Estrogen regulates KiSS1 gene expression through estrogen receptor alpha and SP protein complexes.  

PubMed

Kisspeptins are natural ligands of G protein-coupled receptor-54. Activation of KiSS1/G protein-coupled receptor-54 signaling pathways results in potent activation of the hypothalamus-pituitary-gonadal axis and initiates puberty. Recent data have shown that in female mice, KiSS1 is positively regulated by estradiol (E(2)) in the anteroventral periventricular nucleus, an important reproductive neuroendocrine brain region, but negatively regulated in the arcuate nucleus. However, little is known about the molecular mechanisms governing E(2)-modulated KiSS1 expression. Here, we demonstrate that the expression level of the KiSS1 gene was up-regulated with the administration of E(2) in estrogen receptor alpha (ERalpha)-positive hypothalamic GT1-7 cells. Using transient transfection of human KiSS1 gene promoter coupled to a luciferase reporter, E(2) increases promoter activity in the presence of ERalpha. Deletion analysis of KiSS1 promoter indicates that the E(2)-regulated increase in promoter activity depends on the Sp1 sites of the proximal promoter region. Using both EMSAs and chromatin immunoprecipitation analysis, we determined that both Sp1 and Sp3 proteins constitutively associate with the four putative Sp1 sites in vitro, whereas the association of ERalpha with the KiSS1 promoter is dependent on E(2) exposure. Sp1 and ERalpha form a complex in vivo to mediate the E(2)-induced activation of KiSS1 promoter. Interestingly, Sp1 transactivates KiSS1 promoter activity, whereas Sp3 functions as a transcriptional repressor. Together, these results demonstrate that E(2)-dependent transcriptional activation of KiSS1 gene is mediated by ERalpha through the interaction of Sp1/Sp3 proteins with the GC-rich motifs of KiSS1 promoter, providing a molecular mechanism of how steroid hormone feedback regulates KiSS1 expression. PMID:17656465

Li, Dali; Mitchell, Dianne; Luo, Jian; Yi, Zhengfang; Cho, Sung-Gook; Guo, Jingjing; Li, Xiaoying; Ning, Guang; Wu, Xiushan; Liu, Mingyao

2007-10-01

432

Yeasts associated with the curculionid beetle Xyloterinus politus: Candida xyloterini sp. nov., Candida palmyrensis sp. nov. and three common ambrosia yeasts.  

PubMed

Seven yeast strains were isolated from the body surface and galleries of Xyloterinus politus, the ambrosia beetle that attacks black oak trees. Based on rDNA sequence comparisons and other taxonomic characteristics, five of the strains were identified as members of the species Saccharomycopsis microspora, Wickerhamomyces hampshirensis and Candida mycetangii, which have been reported previously as being associated with insects. The remaining two yeast strains were proposed as representatives of two novel species, Candida xyloterini sp. nov. (type strain ATCC 62898(T)=CBS 11547(T)) and Candida palmyrensis sp. nov. (type strain ATCC 62899(T)=CBS 11546(T)). C. xyloterini sp. nov. is a close sister taxon to Ogataea dorogensis and assimilates methanol as a sole carbon source but lacks ascospores. On the other hand, C. palmyrensis sp. nov. is phylogenetically distinct from any other ambrosia yeast reported so far. The species was placed near Candida sophiae-reginae and Candida beechii based on DNA sequence analyses, but neither of these were close sister taxa to C. palmyrensis sp. nov. PMID:19734291

Suh, Sung-Oui; Zhou, Jianlong

2010-07-01

433

Morphology and phylogeny of two new pleurostomatid ciliates, Epiphyllum shenzhenense n. sp. and Loxophyllum spirellum n. sp. (Protozoa, Ciliophora) from a mangrove wetland, South China.  

PubMed

The morphology, infraciliature, and small subunit (SSU) rRNA gene sequences of two new pleurostomatid ciliates, Epiphyllum shenzhenense n. sp. and Loxophyllum spirellum n. sp., isolated from a mangrove wetland near Shenzhen, South China, were investigated. Epiphyllum shenzhenense n. sp. is morphologically characterized by leaf-shaped cell about 150 x 35 microm in vivo, usually with four contractile vacuoles, 20-29 right kineties and 10-26 left kineties, ca. four macronuclear nodules, and two types of extrusomes (i.e. short spindle-shaped and long bar-shaped). As a new species, L. spirellum n. sp. is distinguished from its congeners by its posterior dorsal margin twisted onto the left side, the distribution of extrusomes (evenly arranged along the oral slit, the posterior end, and clustered to 7-13 warts on dorsal margin), the subterminally positioned contractile vacuole, the number of kineties (8-10 on right side, 4-5 on left side), and its genetic distance from congeners. Phylogenetic trees based on the SSU rRNA gene sequence for both organisms were constructed, which indicate that Epiphyllum is a distinct genus and occupies a basal position in the Pleurostomatida clade; L. spirellum n. sp. falls well into the Loxophyllum clade, which has a close relationship with Litonotus and Spiroloxophyllum. PMID:20735517

Pan, Hongbo; Gao, Feng; Li, Jiqiu; Lin, Xiaofeng; Al-Farraj, Saleh A; Al-Rasheid, Khaled A S

2010-01-01

434

JMJD2A-dependent silencing of Sp1 in advanced breast cancer promotes metastasis by downregulation of DIRAS3.  

PubMed

Specificity protein 1(Sp1) is a ubiquitous transcription factor and is highly expressed in breast cancer. However, its expression pattern and role in breast cancer progression remain unclear. The purpose of this study is to examine the expression pattern of Sp1 and determine its role in breast cancer progression. Immunohistochemistry (IHC) was performed on breast cancer tissues to reveal the expression pattern of Sp1. Spearman rank correlation was used for clinical statistics. Gene and protein expressions were monitored by IHC analysis, quantitative polymerase chain reaction, and Western blot. Wound-healing and Transwell assays were conducted to assess the role of Sp1 in breast cancer. Co-immunoprecipitation, deletion mutagenesis, chromatin immunoprecipitation, and dual luciferase reporter gene assays were used for investigation of the regulatory network. Sp1 expression was downregulated in late stage breast cancer and in highly invasive breast cancer cell lines. Expression of Sp1 was negatively correlated with TNM staging (P = 0.002) and metastasis status (P = 0.023). Overexpression of Sp1 inhibited breast cancer cell migratory and invasive abilities, whereas knockdown of GTP-binding RAS-like 3 (DIRAS3, also known as ARHI, NOEY2) attenuated the inhibitory effects. Moreover, re-expression of DIRAS3 abolished Sp1 knockdown-mediated cell migration and invasion. Jumonji domain containing 2A (JMJD2A) inhibited Sp1 autoregulation and explains Sp1 expression pattern in breast cancer. Sp1 negatively regulated breast cancer metastasis by transcriptional activation of DIRAS3. Inhibition of Sp1 autoregulation by JMJD2A contributed to Sp1 expression pattern in breast cancer. Our findings provided evidence that targeted therapy against Sp1 might be useful in early stage breast cancer. However, in late stages, development of Sp1 activator may be more promising for breast cancer treatments. PMID:25193278

Li, Liliang; Gao, Pan; Li, Yuhua; Shen, Yiwen; Xie, Jianhui; Sun, Daming; Xue, Aimin; Zhao, Ziqin; Xu, Zude; Zhang, Mingchang; Li, Beixu; Jiang, Jieqing

2014-10-01

435

Cosmocercoides himalayanus sp. nov. (Nematoda, Cosmocercidae) in Duttaphrynus himalayanus (Amphibia, Anura) from Dehradun (Uttarakhand), India.  

PubMed

Cosmocercoides himalayanus sp. nov. (Nematoda, Cosmocercidae) from the large intestine of Duttaphrynus himalayanus (Amphibia, Anura) from Dehradun, India is described and illustrated. Cosmocercoides himalayanus sp. nov. represents the 21st species assigned to the genus and the 9th species from the Oriental biogeographical region. Cosmocercoides himalayanus sp. nov. differs from the previously described Oriental species in