Science.gov

Sample records for sp metabolitos secundarios

  1. Cattleya sp. sp. or hybrid (Cultivated) 

    E-print Network

    James R. Manhart

    2011-08-10

    The regulatory mechanisms that govern heterocyst development in Anabaena sp. strain PCC 7120 have been continuously refined over the last two decades. In this work, we show that three of the sigma factor genes present in the Anabaena sp. strain PCC...

  2. Plasmids in Frankia sp.

    PubMed

    Normand, P; Simonet, P; Butour, J L; Rosenberg, C; Moiroud, A; Lalonde, M

    1983-07-01

    A method to achieve cell lysis and isolate Frankia sp. plasmid DNA was developed. A screening of Frankia sp. strains belonging to different host compatibility groups (Alnus sp., Elaeagnus sp., Ceanothus sp.) showed that, of 39 strains tested, 4 (strains Cp11, ARgN22d, ArI3, and EUN1f) possessed plasmids ranging in size from 7.1 to 32.2 kilobase pairs as estimated from agarose gel electrophoresis and electron microscopy. A total of 11 plasmids were detected. PMID:6863219

  3. Pseudomonas kuykendallii sp. nov.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This is a submission to the list of microorganisms with standing in nomenclature maintained by the International Journal of Systematic and Evolutionary Microbiology. We wish to have Pseudomonas kuykendallii sp. nov. added to the list as a valid species belonging to the genus Pseudomonas. Three str...

  4. SP mountain data analysis

    NASA Technical Reports Server (NTRS)

    Rawson, R. F.; Hamilton, R. E.; Liskow, C. L.; Dias, A. R.; Jackson, P. L.

    1981-01-01

    An analysis of synthetic aperture radar data of SP Mountain was undertaken to demonstrate the use of digital image processing techniques to aid in geologic interpretation of SAR data. These data were collected with the ERIM X- and L-band airborne SAR using like- and cross-polarizations. The resulting signal films were used to produce computer compatible tapes, from which four-channel imagery was generated. Slant range-to-ground range and range-azimuth-scale corrections were made in order to facilitate image registration; intensity corrections were also made. Manual interpretation of the imagery showed that L-band represented the geology of the area better than X-band. Several differences between the various images were also noted. Further digital analysis of the corrected data was done for enhancement purposes. This analysis included application of an MSS differencing routine and development of a routine for removal of relief displacement. It was found that accurate registration of the SAR channels is critical to the effectiveness of the differencing routine. Use of the relief displacement algorithm on the SP Mountain data demonstrated the feasibility of the technique.

  5. Laser sculpting of atomic sp, sp(2) , and sp(3) hybrid orbitals.

    PubMed

    Liu, Chunmei; Manz, Jörn; Yang, Yonggang

    2015-01-12

    Atomic sp, sp(2) , and sp(3) hybrid orbitals were introduced by Linus Pauling to explain the nature of the chemical bond. Quantum dynamics simulations show that they can be sculpted by means of a selective series of coherent laser pulses, starting from the 1s orbital of the hydrogen atom. Laser hybridization generates atoms with state-selective electric dipoles, opening up new possibilities for the study of chemical reaction dynamics and heterogeneous catalysis. PMID:25257703

  6. Acetobacter intermedius, sp. nov.

    PubMed

    Boesch, C; Trcek, J; Sievers, M; Teuber, M

    1998-03-01

    Strains of a new species in the genus Acetobacter, for which we propose the name A. intermedius sp. nov., were isolated and characterized in pure culture from different sources (Kombucha beverage, cider vinegar, spirit vinegar) and different countries (Switzerland, Slovenia). The isolated strains grow in media with 3% acetic acid and 3% ethanol as does A. europaeus, do, however, not require acetic acid for growth. These characteristics phenotypically position A. intermedius between A. europaeus and A. xylinus, DNA-DNA hybridizations of A. intermedius-DNA with DNA of the type strains of Acetobacter europaeus, A. xylinus, A. aceti, A. hansenii, A. liquefaciens, A. methanolicus, A. pasteurianus, A. diazotrophicus, Gluconobacter oxydans and Escherichia coli HB 101 indicated less than 60% DNA similarity. The important features of the new species are described. Acetobacter intermedius strain TF2 (DSM11804) isolated from the liquid phase of a tea fungus beverage (Kombucha) is the type strain. PMID:13678040

  7. SP-100 space reactor safety

    SciTech Connect

    Not Available

    1987-05-01

    The SP-100 space reactor power system is being developed to meet the large electrical power requirements of civilian and military missions planned for the 1990's and beyond. It will remove the restrictions on electrical power generation that have tended to limit missions and will enable the fuller exploration and utilization of space. This booklet describes the SP-100 space reactor power system and its development. Particular emphasis is given to safety. The design aand operational features as well as the design and safety review process that will assure that the SP-100 can be launched nd operated safely are described.

  8. Actinoplanes couchii sp. nov.

    PubMed

    Kämpfer, Peter; Huber, Birgit; Thummes, Kathrin; Grün-Wollny, Iris; Busse, Hans-Jürgen

    2007-04-01

    A Gram-positive bacterium, strain GW8-1761(T), was isolated from soil close to the Marmore waterfalls, Terni, Italy. 16S rRNA gene sequence similarity studies showed that strain GW8-1761(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes italicus JCM 3165(T) (98.9 %), A. rectilineatus IFO 13941(T) (98.5 %), A. palleronii JCM 7626(T) (97.8 %), A. utahensis IFO 13244(T) (97.6 %) and A. cyaneus DSM 46137(T) (97.6 %). Strain GW8-1761(T) could be distinguished from any other Actinoplanes species with validly published names by 16S rRNA gene sequence similarity values of less than 97.5 %. Chemotaxonomic data [major menaquinone MK-9(H(4)); major polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol, with phosphatidylcholine and aminoglycolipids absent; major fatty acids C(15 : 0), C(16 : 0), C(16 : 0) iso, C(17 : 1)omega8c and summed feature 3 (C(16 : 1)omega7c and/or C(15 : 0) iso 2-OH)] supported the affiliation of strain GW8-1761(T) to the genus Actinoplanes. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW8-1761(T) from the most closely related species. Strain GW8-1761(T) therefore merits species status, and we propose the name Actinoplanes couchii sp. nov., with the type strain GW8-1761(T) (=DSM 45050(T)=CIP 109316(T)). PMID:17392194

  9. Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera

    PubMed Central

    Alsterfjord, Magnus; Nilson, Bo; Butler, Èile; Vásquez, Alejandra

    2014-01-01

    We previously discovered a symbiotic lactic acid bacterial (LAB) microbiota in the honey stomach of the honeybee Apis mellifera. The microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. 16S rRNA gene sequence analyses and phenotypic and genetic characteristics revealed that the phylotypes isolated represent seven novel species. One grouped with Lactobacillus kunkeei and the others belong to the Lactobacillus buchneri and Lactobacillus delbrueckiisubgroups of Lactobacillus. We propose the names Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov. for these novel species, with the respective type strains being Fhon13NT (?=?DSM 26257T?=?CCUG 63287T), Bin4NT (?=?DSM 26254T?=?CCUG 63291T), Hon2NT (?=?DSM 26255T?=?CCUG 63289T), Hma8NT (?=?DSM 26256T?=?CCUG 63629T), Hma2NT (?=?DSM 26263T?=?CCUG 63633T), Bma5NT (?=?DSM 26265T?=?CCUG 63301T) and Biut2NT (?=?DSM 26262T?=?CCUG 63631T). PMID:24944337

  10. The Sp(1)-Kepler Problems

    E-print Network

    Guowu Meng

    2010-03-05

    Let $n\\ge 2$ be a positive integer. To each irreducible representation $\\sigma$ of $\\mathrm{Sp}(1)$, an $\\mathrm{Sp}(1)$-Kepler problem in dimension $(4n-3)$ is constructed and analyzed. This system is super integrable and when $n=2$ it is equivalent to a generalized MICZ-Kepler problem in dimension five. The dynamical symmetry group of this system is $\\widetilde {\\mathrm O}^*(4n)$ with the Hilbert space of bound states ${\\mathscr H}(\\sigma)$ being the unitary highest weight representation of $\\widetilde {\\mathrm {O}^*}(4n)$ with highest weight $$(\\underbrace{-1, ..., -1}_{2n-1}, -(1+\\bar\\sigma)),$$ which occurs at the right-most nontrivial reduction point in the Enright-Howe-Wallach classification diagram for the unitary highest weight modules. Here $\\bar\\sigma$ is the highest weight of $\\sigma$. Furthermore, it is shown that the correspondence $\\sigma\\leftrightarrow \\mathscr H(\\sigma)$ is the theta-correspondence for dual pair $(\\mathrm{Sp}(1), \\mathrm{O}^*(4n))\\subseteq\\mathrm{Sp}_{8n}(\\mathbb R)$.

  11. The Sp(1)-Kepler problems

    SciTech Connect

    Meng Guowu

    2009-07-15

    Let n{>=}2 be a positive integer. To each irreducible representation {sigma} of Sp(1), an Sp(1)-Kepler problem in dimension (4n-3) is constructed and analyzed. This system is superintegrable, and when n=2 it is equivalent to a generalized MICZ-Kepler problem in dimension of 5. The dynamical symmetry group of this system is O-tilde*(4n) with the Hilbert space of bound states H({sigma}) being the unitary highest weight representation of O*-tilde(4n) with highest weight, (-1,{center_dot}{center_dot}{center_dot},-1,-(1+{sigma})), which occurs at the rightmost nontrivial reduction point in the Enright-Howe-Wallach classification diagram for the unitary highest weight modules. Here {sigma} is the highest weight of {sigma}. Furthermore, it is shown that the correspondence {sigma}{r_reversible}H({sigma}) is the theta-correspondence for dual pair (Sp(1),O*(4n))subset Sp(8n,R)

  12. Argonne's SpEC Module

    SciTech Connect

    Harper, Jason

    2014-05-05

    Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

  13. Argonne's SpEC Module

    ScienceCinema

    Harper, Jason

    2014-06-05

    Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

  14. Leaving the fullerene road: presence and stability of sp chains in sp2

    E-print Network

    Powles, Rebecca

    Leaving the fullerene road: presence and stability of sp chains in sp2 carbon clusters and cluster carbon sp chains are essential ingredients for the formation of carbon fullerenes and nanotubes [6, 7 identified as a fundamental ingredient to transform sp structures into fullerenes and nanotubes

  15. MSc Speech Lab 01 Speech Analysis mwmak:doc/notes/sp/beng/beng_sp_lab01.doc 6 March, 20031

    E-print Network

    Mak, Man-Wai

    MSc Speech Lab 01 Speech Analysis mwmak:doc/notes/sp/beng/beng_sp_lab01.doc 6 March, 20031 THE HONG Speech Lab 01 Speech Analysis mwmak:doc/notes/sp/beng/beng_sp_lab01.doc 6 March, 20032 10) Click Plot Lab 01 Speech Analysis mwmak:doc/notes/sp/beng/beng_sp_lab01.doc 6 March, 20033 Xh = abs

  16. GAMBUSIA INFANS,sp. nov, Salamanca.Xlexico. NOTROPIS CHIHUAHUA, sp. nov. Salamanca.Xlexico.

    E-print Network

    GAMBUSIA INFANS,sp. nov, Salamanca.Xlexico. NOTROPIS CHIHUAHUA, sp. nov. Salamanca.Xlexico. EVARRA uztecus. City of Mexico. Notropis ornatus. Rio Conches. Notropis chihuahua. Rio Conches. Notropis orca

  17. Sphingomonas ginsengisoli sp. nov. and Sphingomonas sediminicola sp. nov.

    PubMed

    An, Dong-Shan; Liu, Qing-Mei; Lee, Hyung-Gwan; Jung, Mi-Seon; Kim, Sun-Chan; Lee, Sung-Taik; Im, Wan-Taek

    2013-02-01

    Two novel bacteria, designated strains Gsoil 634(T) and Dae 20(T), were isolated in South Korea from soil of a ginseng field and freshwater sediment, respectively and were characterized by a polyphasic approach to clarify their taxonomic positions. Phylogenetic analysis based on 16S rRNA gene sequences indicated that, although they probably represented two distinct species (indicated by a sequence similarity of 96.6?%), both strain Gsoil 634(T) and strain Dae 20(T) belonged to the genus Sphingomonas and were most closely related to 'Sphingomonas humi' PB323 (97.8?% and 96.7?% sequence similarity, respectively), Sphingomonas kaistensis PB56(T) (96.8?% and 96.7?%), Sphingomonas astaxanthinifaciens TDMA-17(T) (96.6?% and 95.4?%) and Sphingomonas jaspsi TDMA-16(T) (95.6?% and 95.8?%). For both novel strains, the major ubiquinone was Q-10, the major polyamine was homospermidine, the major cellular fatty acids included summed feature 7 (C(18?:?1)?7c, C(18?:?1)?9t and/or C(18?:?1)?12t), C(17?:?1)?6c and C(16?:?0), and the polar lipids included sphingoglycolipid. These chemotaxonomic data supported the affiliation of both strains to the genus Sphingomonas. However, the DNA-DNA relatedness value between strain Gsoil 634(T) and 'Sphingomonas humi' PB323(T) was 31?%. Moreover, the results of physiological and biochemical tests allowed the phenotypic differentiation of strains Gsoil 634(T) and Dae 20(T) from established members of the genus Sphingomonas. Based on these data, the two isolates represent two novel species in the genus Sphingomonas, for which the names Sphingomonas ginsengisoli sp. nov. (type strain Gsoil 634(T)?=?KCTC 12630(T)?=?DSM 18094(T)?=?LMG 23739(T)) and Sphingomonas sediminicola sp. nov. (type strain Dae 20(T) ?=?KCTC 12629(T)?=?DSM 18106(T)?=?LMG 23592(T)) are proposed. PMID:22505600

  18. Structural and functional differences among human surfactant proteins SP-A1, SP-A2 and co-expressed SP-A1/SP-A2: role of supratrimeric oligomerization

    PubMed Central

    Sánchez-Barbero, Fernando; Rivas, Germán; Steinhilber, Wolfram; Casals, Cristina

    2007-01-01

    SP-A (surfactant protein A) is a membrane-associated SP that helps to maintain the lung in a sterile and non-inflamed state. Unlike SP-As from other mammalian species, human SP-A consists of two functional gene products: SP-A1 and SP-A2. In all the functions examined, recombinant human SP-A1 invariably exhibits lower biological activity than SP-A2. The objective of the present study was to investigate why SP-A2 possesses greater biological activity than SP-A1 and what advantage accrues to having two polypeptide chains instead of one. We analysed structural and functional characteristics of recombinant baculovirus-derived SP-A1, SP-A2 and co-expressed SP-A1/SP-A2 using a wide array of experimental approaches such as analytical ultracentrifugation, DSC (differential scanning calorimetry) and fluorescence. We found that the extent of supratrimeric assembly is much lower in SP-A1 than SP-A2. However, the resistance to proteolysis is greater for SP-A1 than for SP-A2. Co-expressed SP-A1/SP-A2 had greater thermal stability than SP-A1 and SP-A2 and exhibited properties of each protein. On the one hand, SP-A1/SP-A2, like SP-A2, had a higher degree of oligomerization than SP-A1, and consequently had lower Kd for binding to bacterial Re-LPS (rough lipopolysaccharide), higher self-association in the presence of calcium and greater capability to aggregate Re-LPS and phospholipids than SP-A1. On the other hand, SP-A1/SP-A2, like SP-A1, was more resistant to trypsin degradation than SP-A2. Finally, the importance of the supratrimeric assembly for SP-A immunomodulatory function is discussed. PMID:17542781

  19. Early experiences with the IBM SP-1

    SciTech Connect

    Gropp, W.

    1993-06-01

    The IBM SP-1 is IBM`s newest parallel distributed-memory computer. As part of a joint project with IBM, Argonne took delivery of an early system in order to evaluate the software environment and to begin porting programming packages and applications to this machine. This report discusses the results of those early efforts. Despite the newness of the machine and the lack of a fast interprocessor switch (part of the SP-1 but not yet available for the machine), every code that they attempted to port ran on the SP-1 with little or no modification. The report concludes with a discussion of expectations for the fast interconnect.

  20. Bioaccumulation of total and methyl mercury in three earthworm species (Drawida sp., Allolobophora sp., and Limnodrilus sp.).

    PubMed

    Zhang, Zhong Sheng; Zheng, Dong Mei; Wang, Qi Chao; Lv, Xian Guo

    2009-12-01

    We determined total and methyl mercury contents in soil, three earthworm species and their vomitus to study the species-specific differences of mercury bioconcentration in Huludao City, a heavily polluted region by chlor-alkali and nonferrous metal smelting industry in Liaoning Province, northeast China. Total and methyl mercury contents were 7.20 mg/kg and 6.94 ng/g in soil, 1.43 mg/kg and 43.03 ng/g in Drawida sp., 2.80 mg/kg and 336.52 ng/g in Alolobophora sp., respectively. Total mercury contents were 0.966 mg/kg in Drawida sp. vomitus and 4.979 mg/kg in Alolobophora sp. vomitus, respectively. Total mercury contents in earthworms and their vomitus were significantly species-specific different and were both in decreasing with earthworms body lengths, which might due to the growth dilution. Among the soil, earthworms and their vomitus, total mercury contents were in the order of soil > earthworms > earthworm vomitus. Methyl mercury was about 3.01% of total mercury in Drawida sp., 12.02% of total mercury in Alolobophora sp., respectively. It suggested that mercury was mostly in inorganic forms in earthworms. Bioaccumulation factors of methyl mercury from soil to earthworms were much higher than those of total mercury, which suggested that methyl mercury might be more easily absorbed by and accumulated in earthworms because of its lipid solubility. PMID:19779655

  1. Relationship between the Unicellular Red Alga Porphyridium sp. and Its Predator, the Dinoflagellate Gymnodinium sp

    PubMed Central

    Ucko, Michal; Cohen, Ephraim; Gordin, Hillel; Arad, Shoshana (Malis)

    1989-01-01

    Contamination of algae cultivated outdoors by various microorganisms, such as bacteria, fungi, algae, and protozoa, can affect growth and product quality, sometimes causing fast collapse of the cultures. The main contaminant of Porphyridium cultures grown outdoors in Israel is a Gymnodinium sp., a dinoflagellate that feeds on the alga. Comparison of the effects of various environmental conditions, i.e., pH, salinity, and temperature, on Gymnodinium and Porphyridium species revealed that the Gymnodinium sp. has sharp optimum curves, whereas the Porphyridium sp. has a wider range of optimum conditions and is also more resistant to extreme environmental variables. The mode of preying on the alga was observed, and the specificity of the Gymnodinium sp. for the Porphyridium sp. was shown. In addition, Gymnodinium extract was shown to contain enzymatic degrading activity specific to the Porphyridium sp. cell wall polysaccharide. PMID:16348059

  2. Launch vehicle integration requirements for SP-100

    SciTech Connect

    Shaw, L.T. Jr.; Womack, J.R.

    1984-01-31

    SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS) Space Shuttle System into a near-earth orbit, deployed, and through upper stage propulsion burn(s) be inserted/transferred to its mission orbit. The nature of the advanced design SP-100 gives rise to a set of issues that require special attention to assure that payloads using this power plant are physically and functionally compatible with the NSTS and meet the safety requirements thereof. The purpose of this document is to define and present the requirements and interface provisions that, when satisfied, will ensure technical compability between SP-100 systems and the NSTS.

  3. Launch vehicle integration requirements for SP-100

    NASA Technical Reports Server (NTRS)

    Shaw, L. T., Jr.; Womack, J. R.

    1984-01-01

    SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS) Space Shuttle System into a near-earth orbit, deployed, and through upper stage propulsion burn(s) be inserted/transferred to its mission orbit. The nature of the advanced design SP-100 gives rise to a set of issues that require special attention to assure that payloads using this power plant are physically and functionally compatible with the NSTS and meet the safety requirements thereof. The purpose of this document is to define and present the requirements and interface provisions that, when satisfied, will ensure technical compatibility between SP-100 systems and the NSTS.

  4. Silencing the SpMPK1, SpMPK2, and SpMPK3 genes in tomato reduces abscisic acid-mediated drought tolerance.

    PubMed

    Li, Cui; Yan, Jian-Min; Li, Yun-Zhou; Zhang, Zhen-Cai; Wang, Qiao-Li; Liang, Yan

    2013-01-01

    Drought is a major threat to agriculture production worldwide. Mitogen-activated protein kinases (MAPKs) play a pivotal role in sensing and converting stress signals into appropriate responses so that plants can adapt and survive. To examine the function of MAPKs in the drought tolerance of tomato plants, we silenced the SpMPK1, SpMPK2, and SpMPK3 genes in wild-type plants using the virus-induced gene silencing (VIGS) method. The results indicate that silencing the individual genes or co-silencing SpMPK1, SpMPK2, and SpMPK3 reduced the drought tolerance of tomato plants by varying degrees. Co-silencing SpMPK1 and SpMPK2 impaired abscisic acid (ABA)-induced and hydrogen peroxide (H2O2)-induced stomatal closure and enhanced ABA-induced H2O2 production. Similar results were observed when silencing SpMPK3 alone, but not when SpMPK1 and SpMPK2 were individually silenced. These data suggest that the functions of SpMPK1 and SpMPK2 are redundant, and they overlap with that of SpMPK3 in drought stress signaling pathways. In addition, we found that SpMPK3 may regulate H2O2 levels by mediating the expression of CAT1. Hence, SpMPK1, SpMPK2, and SpMPK3 may play crucial roles in enhancing tomato plants' drought tolerance by influencing stomatal activity and H2O2 production via the ABA-H2O2 pathway. PMID:24201128

  5. Expression of surfactant proteins SP-A and SP-D in murine decidua and immunomodulatory effects on decidual macrophages.

    PubMed

    Madhukaran, Shanmuga Priyaa; Koippallil Gopalakrishnan, Aghila Rani; Pandit, Hrishikesh; Marri, Eswari Dodagatta-; Kouser, Lubna; Jamil, Kaiser; Alhamlan, Fatimah S; Kishore, Uday; Madan, Taruna

    2016-02-01

    Surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules that belong to the C-type lectin family. In lungs, they play an important role in the clearance of pathogens and control of inflammation. SP-A and SP-D are also expressed in the female reproductive tract where they play an important role in pregnancy and parturition. However, the role of SP-A and SP-D expressed at the feto-maternal interface (decidua) remains unclear. Here, we have examined the expression of SP-A and SP-D in the murine decidua at 17.5 (pre-parturition) and 19.5dpc (near parturition) and their effect on lipopolysaccharide (LPS)-treated decidual macrophages. SP-A and SP-D were localized to stromal cells in the murine decidua at 17.5 and 19.5dpc in addition to cells lining the maternal spiral artery. Purified pre-parturition decidual cells were challenged with LPS with and without SP-A or SP-D, and expression of F4/80 and TNF-? were measured by flow cytometry. On their own, SP-A or SP-D did not affect the percentage of F4/80 positive cells while they suppressed the percentage of TNF-? positive cells. However, simultaneous addition of SP-A or SP-D, together with LPS, reduced TNF-? secreting F4/80 positive cells. It is likely that exogenous administration of SP-A and SP-D in decidua can potentially control infection and inflammation mediators during spontaneous term labor and infection-induced preterm labor. Thus, the presence of SP-A and SP-D in the murine decidua is likely to play a protective role against intrauterine infection during pregnancy. PMID:26421960

  6. Sp1 cooperates with Sp3 to upregulate MALAT1 expression in human hepatocellular carcinoma.

    PubMed

    Huang, Ziling; Huang, Lanshan; Shen, Siqiao; Li, Jia; Lu, Huiping; Mo, Weijia; Dang, Yiwu; Luo, Dianzhong; Chen, Gang; Feng, Zhenbo

    2015-11-01

    Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), also known as nuclear-enriched transcript 2 (NEAT2), is highly conserved among mammals and highly expressed in the nucleus. It was first identified in lung cancer as a prognostic marker for metastasis but is also associated with several other solid tumors. In hepatocellular carcinoma (HCC), MALAT1 is a novel biomarker for predicting tumor recurrence after liver transplantation. The mechanism of overexpression in tumor progression remains unclear. In the present study, we investigated the role of specificity protein 1/3 (Sp1/3) in regulation of MALAT1 transcription in HCC cells. The results showed a high expression of Sp1, Sp3 and MALAT1 in HCC vs. paired non-tumor liver tissues, which was associated with the AFP level (Sp1, r=7.44, P=0.0064; MALAT1, r=12.37, P=0.0004). Co-silencing of Sp1 and Sp3 synergistically repressed MALAT1 expression. Sp1 binding inhibitor, mithramycin A (MIT), also inhibited MALAT1 expression in HCC cells. In conclusion, the upstream of MALAT1 contains five Sp1/3 binding sites, which may be responsible for MALAT1 transcription. Inhibitors, such as MIT, provide a potential therapeutic strategy for HCC patients with MALAT1 overexpression. PMID:26352013

  7. Artificial surfactants based on analogues of SP-B and SP-C.

    PubMed

    Johansson, J; Curstedt, T; Robertson, B

    2001-01-01

    The hydrophobic proteins SP-B and SP-C are important components of natural surfactant preparations currently used in clinical practice, and physiologically active surfactants can be made from isolated SP-B and/or SP-C reconstituted with synthetic lipids. Efforts have been made to produce these polypeptides, or analogues with similarfunction, by organic synthesis or expression in heterologous systems. It is important to obtain proper folding of the synthetic peptides, as required for optimal interaction with the surfactant lipids. Another issue is to avoid loss of SP-C activity due to alpha-helix to beta-sheet transition. This latter problem can be circumvented by replacing the polyvaline stretch of SP-C with a polyleucine stretch containing a few lysines. Palmitoylation of cysteines or serines at positions 5 and 6 also seems important for the properties of SP-C. SP-B, which is too big a molecule to be easily produced by organic synthesis. apparently can be replaced in an artificial surfactant by a peptide capable of cross-linking phospholipid bilayers. The development of synthetic analogues of the surfacant proteins might make it possible to tailor artificial surfactants for specific therapeutic missions, for instance by enhancing resistance to inactivation by meconium, plasma proteins, or oxygen radicals or maximizing bacteriostatic effects. PMID:11699576

  8. Transformation of Diamond (sp 3 ) to Graphite (sp 2 ) bonds by ionimpact.

    E-print Network

    Adler, Joan

    Transformation of Diamond (sp 3 ) to Graphite (sp 2 ) bonds by ion­impact. David Saada \\Lambda Solid State Institute Technion, Haifa, Israel December 4, 1997 The formation of point defects in diamond experimentally found in radiation­damaged diamond. Keywords: Molecular Dynamics, Structural Defects, Carbon 1 #12

  9. Surfactant Proteins SP-A and SP-D Modulate Uterine Contractile Events in ULTR Myometrial Cell Line

    PubMed Central

    Sotiriadis, Georgios; Dodagatta-Marri, Eswari; Kouser, Lubna; Alhamlan, Fatimah S.; Kishore, Uday; Karteris, Emmanouil

    2015-01-01

    Pulmonary surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules. However, there is extrapulmonary existence, especially in the amniotic fluid and at the feto-maternal interface. There is sufficient evidence to suggest that SP-A and SP-D are involved in the initiation of labour. This is of great importance given that preterm birth is associated with increased mortality and morbidity. In this study, we investigated the effects of recombinant forms of SP-A and SP-D (rhSP-A and rhSP-D, the comprising of trimeric lectin domain) on contractile events in vitro, using a human myometrial cell line (ULTR) as an experimental model. Treatment with rhSP-A or rhSP-D increased the cell velocity, distance travelled and displacement by ULTR cells. rhSP-A and rhSP-D also affected the contractile response of ULTRs when grown on collagen matrices showing reduced surface area. We investigated this effect further by measuring contractility-associated protein (CAP) genes. Treatment with rhSP-A and rhSP-D induced expression of oxytocin receptor (OXTR) and connexin 43 (CX43). In addition, rhSP-A and rhSP-D were able to induce secretion of GRO? and IL-8. rhSP-D also induced the expression of IL-6 and IL-6 Ra. We provide evidence that SP-A and SP-D play a key role in modulating events prior to labour by reconditioning the human myometrium and in inducing CAP genes and pro-inflammatory cytokines thus shifting the uterus from a quiescent state to a contractile one. PMID:26641881

  10. SP-100 flight qualification testing assessment

    NASA Technical Reports Server (NTRS)

    Jeanmougin, Nanette M.; Moore, Roger M.; Wait, David L.; Jacox, Michael G.

    1988-01-01

    The SP-100 is a compact space power system driven by a nuclear reactor that provides 100 kWe to the user at 200 VDC. The thermal energy generated by the nuclear reactor is converted into electrical energy by passive thermoelectric devices. Various options for tailoring the MIL-STD-1540B guidelines to the SP-100 nuclear power system are discussed. This study aids in selecting the appropriate qualification test program based on the cost, schedule, and test effectiveness of the various options.

  11. Developmental expression of Sp1 in the mouse.

    PubMed Central

    Saffer, J D; Jackson, S P; Annarella, M B

    1991-01-01

    The expression of the trans-acting transcription factor Sp1 in mice was defined by a combination of RNA analysis and immunohistochemical localization of the Sp1 protein. Although ubiquitously expressed, there was an unexpected difference of at least 100-fold in the amount of Sp1 message in different cell types. Sp1 protein levels showed corresponding marked differences. Substantial variations in Sp1 expression were also found in some cell types at different stages of development. Sp1 levels appeared to be highest in developing hematopoietic cells, fetal cells, and spermatids, suggesting that an elevated Sp1 level is associated with the differentiation process. These results indicate that Sp1 has a regulatory function in addition to its general role in the transcription of housekeeping genes. Images PMID:2005904

  12. Fungal Diversity Pseudocercospora flavomarginata sp. nov., from Eucalyptus

    E-print Network

    Fungal Diversity Pseudocercospora flavomarginata sp. nov., from Eucalyptus leaves in Thailand Gavin). Pseudocercospora flavomarginata sp. nov. from Eucalyptus leaves in Thailand. Fungal Diversity 22: 71 60 Mycosphaerella species have been linked to leaf diseases on Eucalyptus species, collectively known

  13. The retinoblastoma gene product RB stimulates Sp1-mediated transcription by liberating Sp1 from a negative regulator.

    PubMed Central

    Chen, L I; Nishinaka, T; Kwan, K; Kitabayashi, I; Yokoyama, K; Fu, Y H; Grünwald, S; Chiu, R

    1994-01-01

    Studies have demonstrated that the retinoblastoma susceptibility gene product, RB, can either positively or negatively regulate expression of several genes through cis-acting elements in a cell-type-dependent manner. The nucleotide sequence of the retinoblastoma control element (RCE) motif, GCCACC or CCACCC, and the Sp1 consensus binding sequence, CCGCCC, can confer equal responsiveness to RB. Here, we report that RB activates transcription of the c-jun gene through the Sp1-binding site within the c-jun promoter. Preincubation of crude nuclear extracts with monoclonal antibodies to RB results in reduction of Sp1 complexes in a mobility shift assay, while addition of recombinant RB in mobility shift assay mixtures with CCL64 cell extracts leads to an enhancement of DNA-binding activity of SP1. These results suggest that RB is directly or indirectly involved in Sp1-DNA binding activity. A mechanism by which RB regulates transactivation is indicated by our detection of a heat-labile and protease-sensitive Sp1 negative regulator(s) (Sp1-I) that specifically inhibits Sp1 binding to a c-jun Sp1 site. This inhibition is reversed by addition of recombinant RB proteins, suggesting that RB stimulates Sp1-mediated transactivation by liberating Sp1 from Sp1-I. Additional evidence for Sp1-I involvement in Sp1-mediated transactivation was demonstrated by cotransfection of RB, GAL4-Sp1, and a GAL4-responsive template into CV-1 cells. Finally, we have identified Sp1-I, a approximately 20-kDa protein(s) that inhibits the Sp1 complexes from binding to DNA and that is also an RB-associated protein. These findings provide evidence for a functional link between two distinct classes of oncoproteins, RB and c-Jun, that are involved in the control of cell growth, and also define a novel mechanism for the regulation of c-jun expression. Images PMID:8007947

  14. New anamorphic yeast species: Candida infanticola sp. nov., Candida polysorbophila sp. nov., Candida transvaalensis sp. nov., and Trigonopsis californica sp. nov.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three new species of Candida and a new species of Trigonopsis are described based on their recognition from phylogenetic analysis of gene sequences from large subunit ribosomal RNA, ITS1/ITS2 rRNA, mitochondrial small subunit rRNA and cytochrome oxidase II. Candida infanticola sp. nov. (type strain...

  15. Cochliopodium arabianum n. sp. (Amorphea, Amoebozoa).

    PubMed

    Tekle, Yonas I; Gorfu, Lydia A; Anderson, O Roger

    2015-01-01

    A new species of Cochliopodium isolated from freshwater at Arabia Lake in Lithonia, GA, USA is described based on light microscopic morphology, fine structure, and molecular genetic evidence. Cochliopodium arabianum n. sp., previously labeled as "isolate Con1" in prior publications, has been shown to group within the genus Cochliopodium in our molecular phylogenetic analysis. Light microscopy and fine structure evidence indicates the new isolate not only shares characters of the genus but also unique distinctive features. Cochliopodium arabianum n. sp. is typically round when stationary; or oval to sometimes broadly flabellate or triangular in shape during locomotion, with average length of 35 ?m and breadth of 51 ?m. Fine structure evidence indicates C. arabianum n. sp. has tower-like scales, lacking a terminal spine, sharing high similarity with its closest relative C. actinophorum. However, the scales of C. arabianum n. sp. are unique in height and the breadth of the base plate. Both morphological and molecular data, including SSU-rDNA and COI, indicate that this new species falls in a clade sufficiently different from other species to suggest that it is a valid new species. PMID:25851131

  16. Biogenesis of Lysobacter sp. XL1 vesicles.

    PubMed

    Kudryakova, Irina V; Suzina, Natalia E; Vasilyeva, Natalia V

    2015-09-01

    The Gram-negative bacterium Lysobacter sp. XL1 forms vesicles and, using them, secretes an extracellular protein, bacteriolytic endopeptidase L5. Fractionation of a Lysobacter sp. XL1 vesicle preparation in a sucrose density gradient yielded four vesicle fractions of 30%, 35%, 40% and 45% sucrose. The size of most vesicles concentrated in 30% and 35% sucrose fractions were 40-65 and 65-100 nm, respectively. Electrophoresis and immunoblotting showed vesicles of the 30% fraction differed from those in the other fractions not only in density but also in protein content. Protein L5 was found to be secreted into the extracellular medium only by means of vesicles of the 30% sucrose fraction. Electron microscopic immunocytochemistry of Lysobacter sp. XL1 cells showed protein L5 to be distributed unevenly along the periplasmic space and to be concentrated in certain periplasmic loci adjacent to the outer membrane. It was in those loci where vesiculation occurred. A model of the formation of Lysobacter sp. XL1 vesicles is proposed based on the data obtained. PMID:26296706

  17. _q .. SP-6102 -" IN SYSTEMS ENGINEERING

    E-print Network

    Rhoads, James

    Management Issues in Systems Engineering Spacecraft Systems Engineering: An Introduction to the Process of GSFC SE&I and Management for Manned Space Programs The Systems Engineering Role in Top_¢q .. SP-6102 -" READINGS IN SYSTEMS ENGINEERING Edited by Francis T. Hoban and William M

  18. ORIGINAL PAPER Phaeobacter marinintestinus sp. nov., isolated

    E-print Network

    Bae, Jin-Woo

    ORIGINAL PAPER Phaeobacter marinintestinus sp. nov., isolated from the intestine of a sea cucumber of a sea cucumber (Apostichopus japonicus) collected from Pohang in South Korea. Strain UB-M7T displayed-014-0318-x #12;Keywords Novel species Á Phaeobacter marinintestinus Á Polyphasic taxonomy Á Sea cucumber

  19. Power transmission studies for tethered SP-100

    NASA Technical Reports Server (NTRS)

    Bents, David J.

    1988-01-01

    The tether and/or transmission line connecting the SP-100 to Space Station presents some unorthodox challenges in high voltage engineering, power transmission, and distribution. The line, which doubles as a structural element of this unusual spacecraft, will convey HVDC from SP-100 to the platform in low Earth orbit, and environment where the local plasma is sufficient to cause breakdown of exposed conductors at potentials of only a few hundred volts. Its anticipated several years operation, and continuously accumulating exposure to meteoroids and debris, raises an increasing likelihood that mechanical damage, including perforation, will be sustained in service. The present concept employs an array of gas insulated solid wall aluminum coaxial tubes; a conceptual design which showed basic feasibility of the SP-100 powered Space Station. Practical considerations of launch, deployment and assembly have led to investigation of reel deployable, dielectric insulated coaxial cables. To be competitive, the dielectric would have to operate reliably in a radiation environment under electrical stresses exceeding 50 kV/cm. The SP-100 transmission line high voltage interfaces are also considered.

  20. Power transmission studies for tethered SP-100

    NASA Technical Reports Server (NTRS)

    Bents, David J.

    1988-01-01

    The tether and/or transmission line connecting the SP-100 to space station presents some unorthodox challenges in high voltage engineering, power transmission, and distribution. The line, which doubles as a structural element of this unusual spacecraft, will convey HVDC from SP-100 to the platform in low Earth orbit, and environment where the local plasma is sufficient to cause breakdown of exposed conductors at potentials of only a few hundred volts. Its anticipated several years operation, and continuously accumulating exposure to meteoroids and debris, raises an increasing likelihood that mechanical damage, including perforation, will be sustained in service. The present concept employs an array of gas insulated solid wall aluminum coaxial tubes; a conceptual design which showed basic feasibility of the SP-100 powered space station. Practical considerations of launch, deployment and assembly have lead to investigation of reel deployable, dielectric insulated coaxial cables. To be competitive, the dielectric would have to operate reliably in a radiation environment under electrical stresses exceeding 50 kV/cm. The SP-100 transmission line high voltage interfaces are also considered.

  1. Scaling study for SP-100 reactor technology

    SciTech Connect

    Marshall, A.C.; McKissock, B.; National Aeronautics and Space Administration, Cleveland, OH . Lewis Research Center)

    1989-01-01

    In this study, we explored several ways of extending SP-100 reactor technology to higher power levels. One approach was to use the reference SP-100 pin design and increase the fuel pin length and the number of fuel pins as needed to provide higher capability. The impact on scaling of a modified and advanced SP-100 reactor technology was also explored. Finally, the effect of using alternative power conversion subsystems, with SP-100 reactor technology was investigated. One of the principal concerns for any space-based system is mass; consequently, this study focused on estimating reactor, shield, and total system mass. The RSMASS code (Marshall 1986) was used to estimate reactor and shield mass. Simple algorithms developed at NASA Lewis Research Center were used to estimate the balance of system mass. Power ranges from 100 kWe to 10 MWe were explored assuming both one year and seven years of operation. Thermoelectric, Stirling, Rankine, and Brayton power conversion systems were investigated. The impact on safety, reliability, and other system attributes, caused by extending the technology to higher power levels, was also investigated. 6 refs., 4 figs., 3 tabs.

  2. Remote sensing data of SP mountain and SP lava flow in north-central Arizona

    NASA Technical Reports Server (NTRS)

    Schaber, G. G.; Elachi, C.; Farr, T. G.

    1980-01-01

    Multifrequency airborne radar image data of SP Mountain and SP flow in north-central Arizona were obtained in diverse viewing directions and direct and cross-polarization and compared with surface and aerial photography, Landsat multispectral scanner data, airborne thermal infrared imagery, surface geology, and surface roughness statistics. The extremely blocky, basaltic andesite of SP flow is brighter on direct-polarization K-band images than on cross-polarized images taken simultaneously. This effect is explained by multiple scattering and the strong wavelength dependence of polarization effects caused by the rectilinear basaltic andesite scatters. Two distinct types of surface relief on SP flow, one extremely blocky, the other subdued, are clearly discriminated on the visible and thermal wavelength images but are separable only on the longer wavelength L-band radar image data.

  3. Resistivitet-och IP-mtningar vid sp Hard

    E-print Network

    1 Resistivitet- och IP- mätningar vid �spö Hard Rock Laboratory Erik Fennvik Examensarbeten i universitet 2015 #12;2 #12;3 Resistivitet- och IP-mätningar vid �spö Hard Rock Laboratory Kandidatarbete Erik-mätningar vid �spö Hard Rock Laboratory ERIK FENNVIK Fennvik, E., 2015: Resistivitet­ och IP-mätningar vid �spö

  4. Targeting Sp1 transcription factors in prostate cancer therapy.

    PubMed

    Sankpal, Umesh T; Goodison, Steven; Abdelrahim, Maen; Basha, Riyaz

    2011-09-01

    Transcription factors are proteins that regulate gene expression by binding to specific DNA sequences within gene promoter regions. Specificity protein (Sp) family transcription factors play a critical role in various cellular processes and have been shown to be associated with tumorigenesis. The Sp family consists of several members that contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus and serine/threonine- and glutamine-rich transactivation domains at the N-terminal. Sp1 is elevated in several cancers including prostate and is associated with the prognosis of patients. Sp1, Sp3, and Sp4 regulate a variety of cancer associated genes that are involved in cell cycle, proliferation, cell differentiation, and apoptosis. Studies have shown that in prostate cancer, Sp1 regulates important genes like androgen receptor, TGF-?, c-Met, fatty acid synthase, matrix metalloprotein (MT1-MMP), PSA, and ?-integrin. These results highlight the importance of Sp1 in prostate cancer and emphasize the potential therapeutic value of targeting Sp1. Several strategies, including the use of natural and synthetic compounds, have been used to inhibit Sp1 in prostate cancer. These include polyphenol quercetin, betulinic acid, acetyl-11-keto-beta-boswellic acid, tea phenols, isothiocyanates, thiazolidinediones, arsenic trioxide, and selenium. This review will describe the association of Sp proteins in prostate cancer with a special emphasis on some of the agents tested to target Sp proteins for the treatment of this malignancy. PMID:22022994

  5. 76 FR 22694 - SP 49 Pipeline LLC; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-22

    ... Energy Regulatory Commission SP 49 Pipeline LLC; Notice of Filing Take notice that on April 12, 2011, SP 49 Pipeline LLC (``SP 49'') submitted a request for waiver of the requirement to file the FERC Form... Pipe Line Company's (``Chevron'') pipeline system and associated equipment, specifically the South...

  6. Polycyclic aromatic hydrocarbons in water, mussels ( Brachidontes sp., Tagelus sp.) and fish ( Odontesthes sp.) from Bahía Blanca Estuary, Argentina

    NASA Astrophysics Data System (ADS)

    Arias, Andres H.; Spetter, Carla V.; Freije, Rubén H.; Marcovecchio, Jorge E.

    2009-10-01

    Polycyclic aromatic hydrocarbons (PAHs), one of the major groups of anthropogenic environmental pollutants, were firstly identified and measured in coastal waters, native mussels and fish of an industrialized South American estuary. The aim of this study was to measure the PAHs concentrations and evaluate distribution and sources in surface seawater, native mussels ( Brachidontes sp. and Tagelus sp.) and fish ( Odontesthes sp.) from various coastal sites of the Bahía Blanca Estuary, in order to put the estuary in a global context and assess it ecotoxicological risk. For this, 40 samples of surface water were collected between August 2004 and February 2006 and analyzed by GC/MS for 17 PAHs, including the 16 EPA priority PAHs. In addition, main physicochemical parameters, nutrients and pigments were simultaneously obtained as key parameters to evaluate possible relationships. Finally, several native mussel communities and fish specimens were also analyzed for PAHs contents. The area of study is located at the Argentinean coast and presents an intensive human and industrial activity at the north shoreline. Total PAHs (?17 PAHs) in whole surface waters ranged from undetected to more than 4 ?g/l. Seasonal PAHs levels relationship with master parameters suggested a relationship between the phytoplankton microbiological cycling and the dissolved/suspended PAHs occurrence in water. In addition, Cluster Analysis was performed to identify the homogeneous groups of individual PAHs in water and Principal Component Analysis to extract underlying common factors. The result of PCA was similar to that of the cluster analysis; both could differentiate two subsets of pyrogenic sources and a petrogenic origin. PAHs levels in indigenous mussels ( Brachidontes sp. and Tagelus sp.) ranged from 348 to 1597 ng/g (dry weight). Fish specimens ( Odontesthes sp.) showed a mean concentration of 1095 ng/g d.w. in whole tissues. Mussel source ratios and PCA results were in good agreement with the dominant sources assessed for water samples and further enabled the linking of PAHs origins according to the sample location. The Bahía Blanca Estuary wide mean of the PAHs burden in mussels showed that the average PAH contamination level was within the "moderate" category. Levels appeared as ecotoxicologically risk safe for the inner stations; however, they were exceeding various safety criterions at the outer. Results indicate the needing of a systematic monitoring program for the area of study, in order to provide accurately assessment and management of risks for the regional population.

  7. Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1

    SciTech Connect

    Ito, Tatsuo; Kitamura, Haruka; Uwatoko, Chisana; Azumano, Makiko; Itoh, Kohji; Kuwahara, Jun

    2010-12-10

    Research highlights: {yields} Sp1 zinc fingers themselves interact with importin {alpha}. {yields} Sp1 zinc finger domains play an essential role as a nuclear localization signal. {yields} Sp1 can be transported into the nucleus in an importin-dependent manner. -- Abstract: Transcription factor Sp1 is localized in the nucleus and regulates the expression of many cellular genes, but the nuclear transport mechanism of Sp1 is not well understood. In this study, we revealed that GST-fused Sp1 protein bound to endogenous importin {alpha} in HeLa cells via the Sp1 zinc finger domains, which comprise the DNA binding domain of Sp1. It was found that the Sp1 zinc finger domains directly interacted with a wide range of importin {alpha} including the armadillo (arm) repeat domain and the C-terminal acidic domain. Furthermore, it turned out that all three zinc fingers of Sp1 are essential for binding to importin {alpha}. Taken together, these results suggest that the Sp1 zinc finger domains play an essential role as a NLS and Sp1 can be transported into the nucleus in an importin-dependent manner even though it possesses no classical NLSs.

  8. Sp6 and Sp8 Transcription Factors Control AER Formation and Dorsal-Ventral Patterning in Limb Development

    PubMed Central

    Haro, Endika; Delgado, Irene; Junco, Marisa; Yamada, Yoshihiko; Mansouri, Ahmed; Oberg, Kerby C.; Ros, Marian A.

    2014-01-01

    The formation and maintenance of the apical ectodermal ridge (AER) is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6?/?;Sp8+/?) exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/?catenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning. PMID:25166858

  9. Sp6 and Sp8 transcription factors control AER formation and dorsal-ventral patterning in limb development.

    PubMed

    Haro, Endika; Delgado, Irene; Junco, Marisa; Yamada, Yoshihiko; Mansouri, Ahmed; Oberg, Kerby C; Ros, Marian A

    2014-08-01

    The formation and maintenance of the apical ectodermal ridge (AER) is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6-/-;Sp8+/-) exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/?catenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning. PMID:25166858

  10. SP-100 inert gas act activation

    SciTech Connect

    Wilcox, A.D.

    1991-09-01

    As part of the SP-100 test program at the US Department of Energy Field Office, Richland, there are plans to test the SP-100 space reactor in a vacuum in the test facility shown in Figure 1. The vacuum vessel will be in an inert gas atmosphere in the reactor experiment (RX) cell. The upper assembly (UA)/pump cells will also be inerted. The objective is to determine whether the radioactivity levels in the facility exhaust are within permissible levels. This radioactivity comes from leakage of activation products from the inert gas cells into the facility ventilation exhaust stream. The specific activities were calculated for the activation products from the combinations of inert gases that were considered for this facility, for a range of leakage rates, and for leakage from the UA/pump cells into the RX cell, and results are detailed in this report.

  11. Carbon sp chains in graphene nanoholes

    NASA Astrophysics Data System (ADS)

    Castelli, Ivano E.; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

    2012-03-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment.

  12. SP-100 design, safety, and testing

    NASA Technical Reports Server (NTRS)

    Cox, Carl. M.; Mahaffey, Michael M.; Smith, Gary L.

    1991-01-01

    The SP-100 Program is developing a nuclear reactor power system that can enhance and/or enable future civilian and military space missions. The program is directed to develop space reactor technology to provide electrical power in the range of tens to hundreds of kilowatts. The major nuclear assembly test is to be conducted at the Hanford Site near Richland, Washington, and is designed to validate the performance of the 2.4-MWt nuclear and heat transport assembly.

  13. Syrian hamsters (Mesocricetus auratus) with simultaneous intestinal Giardia sp., Spironucleus sp., and trichomonad infections.

    PubMed

    Sheppard, Barbara J; Stockdale Walden, Heather D; Kondo, Hirotaka

    2013-11-01

    A commercial facility producing hamsters with a history of infection by dwarf tapeworm (Hymenolepis nana) submitted 15 animals for necropsy and postmortem parasitological and microscopic examination. No tapeworms were detected grossly or microscopically. Fecal examination including gastrointestinal mucosal smears demonstrated mixed intestinal bacteria and low numbers of Giardia sp. Histologic examination of small intestine demonstrated filling of the small intestinal crypts by large numbers of 7-9 µm × 3 µm, rod to crescent or teardrop-shaped flagellates consistent with Spironucleus sp. These organisms had two 1-µm, basophilic, oval nuclei and multiple superficial flagella-like structures. Much larger 10-15 µm × 8-10 µm, oval to pear-shaped organisms were also present in lower numbers and usually located with the crypts. These larger flagellates had multiple flagella and a basophilic rod-shaped nucleus. The larger flagellates included Giardia sp., which had an intimate interface with the surface of the mucosal epithelium, bilaterally symmetry, and binucleation. Lower numbers of trichomonads were also present and were distinguished by an undulating surface membrane and a single nucleus. The mucosa was hyperplastic and moderately inflamed. Although the tapeworm infection was resolved, diagnosis of multiple intestinal flagellates by fecal examination is complicated by the varying sensitivity and diagnostic accuracy of different types of fecal analysis for different flagellate types. Key differences in the morphology and location of the different types of flagellates as observed by histology of intestinal tissues provide important additional diagnostic information to distinguish trichomonads, Spironucleus sp., and Giardia sp. PMID:24081933

  14. Differential effects of human SP-A1 and SP-A2 variants on phospholipid monolayers containing surfactant protein B

    PubMed Central

    Wang, Guirong; Taneva, Svetla; Keough, Kevin M.W.; Floros, Joanna

    2010-01-01

    Summary Surfactant protein A (SP-A), the most abundant protein in the lung alveolar surface, has multiple activities, including surfactant-related functions. SP-A is required for the formation of tubular myelin and the lung surface film. The human SP-A locus consists of two functional SP-A genes, SP-A1 and SP-A2, with a number of alleles characterized for each gene. We have found that the human in vitro expressed variants, SP-A1 (6A2) and SP-A2 (1A0), and the coexpressed SP-A1/SP-A2 (6A2/1A0) protein have a differential influence on the organization of phospholipid monolayers containing surfactant protein B (SP-B). Lipid films containing SP-B and SP-A2 (1A0) showed surface features similar to those observed in lipid films with SP-B and native human SP-A. Fluorescence images revealed the presence of characteristic fluorescent probe-excluding clusters coexisting with the traditional lipid liquid-expanded and liquid-condensed phase. Images of the films containing SP-B and SP-A1 (6A2) showed different distribution of the proteins. The morphology of lipid films containing SP-B and the coexpressed SP-A1/SP-A2 (6A2/1A0) combined features of the individual films containing the SP-A1 or SP-A2 variant. The results indicate that human SP-A1 and SP-A2 variants exhibit differential effects on characteristics of phospholipid monolayers containing SP-B. This may differentially impact surface film activity. PMID:17678872

  15. SpAHA1 and SpSOS1 Coordinate in Transgenic Yeast to Improve Salt Tolerance

    PubMed Central

    Duan, Ruijun; Hao, Gangping; Guo, Jianchun; Jiang, Xingyu

    2015-01-01

    In plant cells, the plasma membrane Na+/H+ antiporter SOS1 (salt overly sensitive 1) mediates Na+ extrusion using the proton gradient generated by plasma membrane H+-ATPases, and these two proteins are key plant halotolerance factors. In the present study, two genes from Sesuvium portulacastrum, encoding plasma membrane Na+/H+ antiporter (SpSOS1) and H+-ATPase (SpAHA1), were cloned. Localization of each protein was studied in tobacco cells, and their functions were analyzed in yeast cells. Both SpSOS1 and SpAHA1 are plasma membrane-bound proteins. Real-time polymerase chain reaction (PCR) analyses showed that SpSOS1 and SpAHA1 were induced by salinity, and their expression patterns in roots under salinity were similar. Compared with untransformed yeast cells, SpSOS1 increased the salt tolerance of transgenic yeast by decreasing the Na+ content. The Na+/H+ exchange activity at plasma membrane vesicles was higher in SpSOS1-transgenic yeast than in the untransformed strain. No change was observed in the salt tolerance of yeast cells expressing SpAHA1 alone; however, in yeast transformed with both SpSOS1 and SpAHA1, SpAHA1 generated an increased proton gradient that stimulated the Na+/H+ exchange activity of SpSOS1. In this scenario, more Na+ ions were transported out of cells, and the yeast cells co-expressing SpSOS1 and SpAHA1 grew better than the cells transformed with only SpSOS1 or SpAHA1. These findings demonstrate that the plasma membrane Na+/H+ antiporter SpSOS1 and H+-ATPase SpAHA1 can function in coordination. These results provide a reference for developing more salt-tolerant crops via co-transformation with the plasma membrane Na+/H+ antiporter and H+-ATPase. PMID:26340746

  16. Direct transesterification of Oedogonium sp. oil be using immobilized isolated novel Bacillus sp. lipase.

    PubMed

    Sivaramakrishnan, Ramachandran; Muthukumar, Karuppan

    2014-01-01

    This work emphasizes the potential of the isolated Bacillus sp. lipase for the production of fatty acid methyl ester by the direct transesterification of Oedogonium sp. of macroalgae. Dimethyl carbonate was used as the extraction solvent and also as the reactant. The effect of solvent/algae ratio, water addition, catalyst, temperature, stirring and time on the direct transesterification was studied. The highest fatty acid methyl ester yield obtained under optimum conditions (5 g Oedogonium sp. powder, 7.5 ml of solvent (dimethyl carbonate)/g of algae, 8% catalyst (%wt/wt of oil), distilled water 1% (wt/wt of algae), 36 h, 55°C and 180 rpm) was 82%. Final product was subjected to thermogravimetric analysis and (1)H NMR analysis. The results showed that the isolated enzyme has good potential in catalyzing the direct transesterification of algae, and the dimethyl carbonate did not affect the activity of the isolated lipase. PMID:23890544

  17. Salt stress tolerance of methylotrophic bacteria Methylophilus sp. and Methylobacterium sp. isolated from coal mine spoils.

    PubMed

    Giri, Deen Dayal; Kumar, Ajay; Shukla, Prabhu Nath; Singh, Ritu; Singh, P K; Pandey, Kapil Deo

    2013-01-01

    Two methylotrophic strains of Bina coalmine spoil BNV7b and BRV25 were identified based on physiological traits and 16S rDNA sequence as Methylophilus and Methylobacterium species.' The strains exhibited similar carbon utilization but differed in N utilization and their response to the metabolic inhibitors. Methylophilus sp. was less tolerant to salt stress and it viability declined to one tenth within 4 h of incubation in 2M NaCI due to membrane damage and leakage of the intracellular electrolytes as evident from malondiaaldehyde (MDA) assay. In 200 mM NaCI, they exhibited increased superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity while in 500 mM NaCI, enzyme activities declined in Methylophilus sp. and increased in Methylobacterium sp. Among exogenously applied osmoprotectants proline was most efficient; however, polyols (mannitol, sorbitol and glycerol) also supported growth under lethal NaCI concentration. PMID:24459832

  18. Sp1 and Sp3 Are important regulators of AP-2gamma gene transcription.

    PubMed

    Li, Mei; Kellems, Rodney E

    2003-10-01

    AP-2gamma is a member of the AP-2 transcription factor family, is highly enriched in the trophoblast cell lineage, and is essential for placental development. In an effort to identify factors regulating AP-2gamma gene expression, we isolated and characterized the promoter and 5'-flanking region of the mouse and human AP-2gamma genes. The transcription start site of the mouse AP-2gamma gene was mapped by primer extension and 5' rapid amplification of cDNA ends. Deletion analysis of the 5'-flanking region revealed a 704-base pair (bp) sequence located approximately 6 kilobases (kb) upstream of the transcription start site that is required for enhanced expression in trophoblast cells. Additional gene transfer studies showed that basal promoter activity resides within a highly conserved, approximately 200-bp DNA sequence located immediately upstream of the transcription start site. The conserved region is highly GC-rich and lacks typical TATA or CCAAT boxes. Multiple potential Sp- and AP-2-binding sites are clustered within this region. Electrophoretic mobility shift assays demonstrated that Sp1 and Sp3 bind to three sites in the promoter region of the mouse AP-2gamma gene. Combined mutation of the three putative Sp sites reduced promoter activity by 80% in trophoblast and nontrophoblast cells, demonstrating the functional importance of these sites in regulating AP-2gamma gene expression. In summary, we have identified a potential trophoblast cell-specific regulatory element located approximately 6 kb upstream of the murine AP-2gamma gene transcription start site, and we have shown that Sp1 and Sp3 bind to cis-regulatory elements located in the promoter proximal region and contribute to basal promoter activity. PMID:12801994

  19. Sulfitobacter geojensis sp. nov., Sulfitobacter noctilucae sp. nov., and Sulfitobacter noctilucicola sp. nov., isolated from coastal seawater.

    PubMed

    Kwak, Min-Jung; Lee, Jung-Sook; Lee, Keun Chul; Kim, Kwang Kyu; Eom, Mi Kyung; Kim, Byung Kwon; Kim, Jihyun F

    2014-11-01

    Four Gram-stain-negative, aerobic, rod-shaped bacterial strains, MM-124, MM-126, NB-68 and NB-77, were isolated from the coastal seawater or a region with a bloom of sea sparkle around Geoje island in Korea. The sequence similarity values of the 16S rRNA gene between the isolates and Sulfitobacter mediterraneus DSM 12244(T) ranged from 97.7 to 98.2%, and phylogenetic relationships suggested that they belong to a phylogenetic branch that includes the genera Sulfitobacter and Roseobacter. The isoprenoid quinone of all three novel strains was ubiquinone-10 and the major fatty acid was cis-vaccenic acid, as in other species of the genus Sulfitobacter. However, there were several differences in the morphological, physiological and biochemical characteristics among the four strains and the reference species of the genus Sulfitobacter. Moreover, the average nucleotide identity values between the three sequenced isolates and the reference strains were below 76.33, indicating that genomic variation exists between the isolates and reference strains. Chemotaxonomic characteristics together with phylogenetic affiliations and genomic distances illustrate that strains MM-124, NB-68 and NB-77 represent novel species of the genus Sulfitobacter, for which the names Sulfitobacter geojensis sp. nov. (type strain MM-124(T)?=KCTC 32124(T)?=JCM 18835(T)), Sulfitobacter noctilucae sp. nov. (type strain NB-68(T)?=KCTC 32122(T)?=JCM 18833(T)) and Sulfitobacter noctilucicola sp. nov. (type strain NB-77(T)?=KCTC 32123(T)?=JCM 18834(T)) are proposed. PMID:25122614

  20. Isolation of C11 Cyclopentenones from Two Didemnid Species, Lissoclinum sp. and Diplosoma sp.

    PubMed Central

    Ogi, Takayuki; Margiastuti, Palupi; Teruya, Toshiaki; Taira, Junsei; Suenaga, Kiyotake; Ueda, Katsuhiro

    2009-01-01

    A series of new C11 cyclopentenones 1–7 was isolated, together with four known metabolites 9/10, 12 and 13, from the extract of the didemnid ascidian Lissoclinum sp. The other didemnid ascidian Diplosoma sp. contained didemnenones 1, 2 and 5, and five known metabolites 8–12. The structures of 1–7 were elucidated by spectroscopic analyses. Cytotoxicity of the isolated compounds was evaluated against three human cancer cell lines (HCT116, A431 and A549). PMID:20098612

  1. O-linked N-acetylglucosaminylation of Sp1 interferes with Sp1 activation of glycolytic genes.

    PubMed

    Lim, Kihong; Yoon, Bo Hyun; Ha, Chang Hoon

    2015-12-01

    Glycolysis, the primary pathway metabolizing glucose for energy production, is connected to the hexosamine biosynthetic pathway (HBP) which produces UDP-N-acetylglucosamine (UDP-GlcNAc), a GlcNAc donor for O-linked GlcNAc modification (O-GlcNAc), as well as for traditional elongated glycosylation. Thus, glycolysis and O-GlcNAc are intimately associated. The present study reports the transcriptional activation of glycolytic genes by the transcription factor Sp1 and the O-GlcNAc-mediated suppression of Sp1-dependent activation of glycolytic genes. O-GlcNAc-deficient mutant Sp1 stimulated the transcription of nine glycolytic genes and cellular production of pyruvate, the final product of glycolysis, to a greater extent than wild-type Sp1. Consistently, this mutant Sp1 increased the protein levels of the two key glycolytic enzymes, phosphofructokinase (PFK) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), to a greater extent than wild-type Sp1. Finally, the mutant Sp1 occupied GC-rich elements on PFK and GAPDH promoters more efficiently than wild-type Sp1. These results suggest that O-GlcNAcylation of Sp1 suppresses Sp1-mediated activation of glycolytic gene transcription. PMID:26499076

  2. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource

    PubMed Central

    Tiwari, Ratnakar; Srivastava, Vikas

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for anticancer potential using human colon adenocarcinoma (HT29) and human kidney adenocarcinoma (A498) cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC) eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer) and MCF-10A (normal human epithelial) exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of anticancer drug. Identification of biomolecules from these extracts are in progress. PMID:26325186

  3. SP-100, a project manager's view

    NASA Technical Reports Server (NTRS)

    Truscello, Vincent C.

    1983-01-01

    Born to meet the special needs of America's space effort, the SP-100 Program testifies to the cooperation among government agencies. The Department of Energy (DOE), the National Aeronautics and Space Administration (NASA), and the Defense Advanced Research Projects Agency (DARPA) are working together to produce a 100-kW power system for use in outer space. At this point in the effort, it is appropriate to review: The approach to meet program goals; the status of activities of the Project Office, managed by the Jet Propulsion Laboratory (JPL); and, because this is a meeting on materials, answers beings developed by the Project Office to vital questions on refractory alloy technology.

  4. Clinical problems of sloths (Bradypus sp. and Choloepus sp.) in captivity.

    PubMed

    Diniz, L S; Oliveira, P M

    1999-03-01

    A 20-yr retrospective study of disease prevalence was carried out for 51 sloths (34 Bradypus sp. and 17 Choloepus sp.) at the São Paulo Zoo. A total of 81 clinical disorders were detected, including nutritional (45.7%), digestive (12.3%), and respiratory (12.3%) problems and injuries (6.1%). A definitive diagnosis was not possible in 8.6% of the cases. The incidence of disease varied according to seasonal climate (winter, 32.5%; spring, 24%; summer, 22.9%; autumn, 20.5%), time in captivity (96.4% of diseases occurred within the first 6 mo and 3.6% occurred thereafter), and type of enclosure (quarantine cage, 96.4%; exhibition enclosure, 3.6%). Both young animals (86.7%) and adults (3.2%) were affected. Parasites were identified by fecal examination in 45.4% of animals with clinical illness (Ascaris sp., 80%; Coccidia sp., 20%). Bacteria such as Salmonella enteritidis, Escherichia coli, and Citrobacter freundii were isolated from feces and/or organs. The first 6 mo in captivity are critical for these animals. Proper management and early identification of medical conditions in captivity have implications for sloth population in the wild. PMID:10367647

  5. Longidorus grandis n. sp. and L. paralongicaudatus n. sp. (Nematoda: Longidoridae), Two Parthenogenetic Species from Arkansas

    PubMed Central

    Ye, Weimin; Robbins, R. T.

    2003-01-01

    Two new parthenogenetic species of Longidorus were found in Arkansas. Longidorus grandis n. sp. is characterized by its body (5.80-8.24 mm), slightly offset head, head width 20-27 µm, odontostyle 86-100 µm, guide ring 26-35 µm posterior to the anterior end, short conoid to mammiliform tail. Longidorus grandis n. sp. is similar to L. vineacola Sturhan &Weischer, 1964; L. lusitanicus Macara, 1985; L. edmundsi Hunt &Siddiqi, 1977; L. kuiperi Brinkman, Loof &Barbez, 1987; L. balticus Brzeski, Peneva &Brown, 2000; L. closelongatus Stoyanov, 1964; and L. seinhorsti Peneva, Loof &Brown, 1998. Longidorus paralongicaudatus n. sp. is characterized by its body length (2.60-5.00 µm), anteriorly flattened and offset head region 13-18 µm wide, odontostyle length 92-127 µm, guide ring 21-30 µm posterior to the anterior end, tail elongate-conical, and c' = 1.2-2.6. Longidorus paralongicaudatus n. sp. most closely resembles L. longicaudatus Siddiqi, 1962; L. socialis Singh &Khan, 1996; L. juvenilis Dalmasso, 1969; and L. curvatus Khan, 1986. PMID:19262768

  6. Calcium Carbonate Formation by Synechococcus sp. Strain PCC 8806 and Synechococcus sp. Strain PCC 8807

    SciTech Connect

    Lee, Brady D.; William A. Apel; Michelle R. Walton

    2006-12-01

    Precipitation of CaCO3 catalyzed by the growth and physiology of cyanobacteria in the Genus Synechococcus represents a potential mechanism for sequestration of CO2 produced during the burning of coal for power generation. Microcosm experiments were performed in which Synechococcus sp. strain PCC 8806 and Synechococcus sp. strain PCC 8807 were tested for their ability to calcify when exposed to a fixed calcium concentration of 3.4 mM and bicarbonate concentrations of 0.5, 1.25 and 2.5 mM. Disappearance of soluble calcium was used as an indicator of CaCO3 formation; results from metabolically active microcosms were compared to controls with no cells or no carbonate added. Synechococcus sp. strain PCC 8806 removed calcium continuously over the duration of the experiment with approximately 18.6 mg of calcium in the solid phase. Calcium removal occurred over a two-day time period when Synechococcus sp. strain PCC 8807 was tested and only 8.9 mg of calcium was removed in the solid phase. The ability of the cyanobacteria to create an alkaline growth environment appeared to be the primary factor responsible for CaCO3 precipitation in these experiments. Removal of inorganic carbon by fixation into biomass was insignificant compared to the mass of inorganic carbon removed by incorporation into the growing CaCO3 solid.

  7. Tubulideres seminoli gen. et sp. nov. and Zelinkaderes brightae sp. nov. (Kinorhyncha, Cyclorhagida) from Florida

    NASA Astrophysics Data System (ADS)

    Sørensen, Martin V.; Heiner, Iben; Ziemer, Ole; Neuhaus, Birger

    2007-12-01

    One new kinorhynch genus and species and one new species from the genus Zelinkaderes are described from sandy sediment off Fort Pierce, Florida. The new genus and species, Tubulideres seminoli gen. et sp. nov. is characterized by the presence of the first trunk segment consisting of a closed ring, the second segment of a bent tergal plate with a midventral articulation and the following nine segments consisting of a tergal and two sternal plates. Cuspidate spines are not present, but flexible tubules are located on several segments, and in particular concentrated on the ventral side of the second segment. Middorsal spines are present on all trunk segments and are alternatingly offset to a position slightly lateral to the middorsal line. Zelinkaderes brightae nov. sp. is characterized by its spine formula in having middorsal spines on trunk segments 4, 6 and 8-11, lateroventral acicular spines on segment 2, lateral accessory cuspidate spines on segments 2 and 8, ventrolateral cuspidate spines on segments 4-6 and 9, lateroventral acicular spines present on segments 8 and 9, and midterminal, lateral terminal and lateral terminal accessory spines on segment 11. The spine formula of Z. brightae nov. sp. places it in a position in between Z. submersus and a clade consisting of Z. klepali and Z. floridensis. The new findings on Z. brightae nov. sp. have led us to propose an emended diagnosis for the genus.

  8. Indirect Manganese Removal by Stenotrophomonas sp. and Lysinibacillus sp. Isolated from Brazilian Mine Water

    PubMed Central

    Barboza, Natália Rocha; Amorim, Soraya Sander; Santos, Pricila Almeida; Reis, Flávia Donária; Cordeiro, Mônica Mendes; Guerra-Sá, Renata; Leão, Versiane Albis

    2015-01-01

    Manganese is a contaminant in the wastewaters produced by Brazilian mining operations, and the removal of the metal is notoriously difficult because of the high stability of the Mn(II) ion in aqueous solutions. To explore a biological approach for removing excessive amounts of aqueous Mn(II), we investigated the potential of Mn(II) oxidation by both consortium and bacterial isolates from a Brazilian manganese mine. A bacterial consortium was able to remove 99.7% of the Mn(II). A phylogenetic analysis of isolates demonstrated that the predominant microorganisms were members of Stenotrophomonas, Bacillus, and Lysinibacillus genera. Mn(II) removal rates between 58.5% and 70.9% were observed for Bacillus sp. and Stenotrophomonas sp. while the Lysinibacillus isolate 13P removes 82.7%. The catalytic oxidation of Mn(II) mediated by multicopper oxidase was not properly detected; however, in all of the experiments, a significant increase in the pH of the culture medium was detected. No aggregates inside the cells grown for a week were found by electronic microscopy. Nevertheless, an energy-dispersive X-ray spectroscopy of the isolates revealed the presence of manganese in Stenotrophomonas sp. and Lysinibacillus sp. grown in K medium. These results suggest that members of Stenotrophomonas and Lysinibacillus genera were able to remove Mn(II) by a nonenzymatic pathway. PMID:26697496

  9. Environmental interactions and the SP-100 power system

    NASA Technical Reports Server (NTRS)

    Ferguson, Dale C.

    1993-01-01

    Interactions of the SP-100 power system with its expected ambient environments are defined. SP-100 payloads will float 100 V negative of the low Earth orbit (LEO) plasma. Choice of proper geometries and materials will prevent arcing at conductor-insulator junctions in LEO. Care in selecting surface coatings will prevent dielectric breakdown. Sputtering is a concern for long-duration LEO missions. Atomic oxygen durability of SP-100 materials will be tested in ground and flight tests. Evaluation of SP-100 in lunar and planetary environments has begun. The report of a recent workshop on Chemical and Electrical Interactions on Mars identified many of the primary interactions.

  10. IBM SP high-performance networking with a GRF.

    SciTech Connect

    Navarro, J.P.

    1999-05-27

    Increasing use of highly distributed applications, demand for faster data exchange, and highly parallel applications can push the limits of conventional external networking for IBM SP sites. In technical computing applications we have observed a growing use of a pipeline of hosts and networks collaborating to collect, process, and visualize large amounts of realtime data. The GRF, a high-performance IP switch from Ascend and IBM, is the first backbone network switch to offer a media card that can directly connect to an SP Switch. This enables switch attached hosts in an SP complex to communicate at near SP Switch speeds with other GRF attached hosts and networks.

  11. Auxiliary-assisted palladium-catalyzed arylation and alkylation of sp2 and sp3 carbon-hydrogen bonds.

    PubMed

    Shabashov, Dmitry; Daugulis, Olafs

    2010-03-24

    We have developed a method for auxiliary-directed, palladium-catalyzed beta-arylation and alkylation of sp(3) and sp(2) C-H bonds in carboxylic acid derivatives. The method employs a carboxylic acid 2-methylthioaniline- or 8-aminoquinoline amide substrate, aryl or alkyl iodide coupling partner, palladium acetate catalyst, and an inorganic base. By employing 2-methylthioaniline auxiliary, selective monoarylation of primary sp(3) C-H bonds can be achieved. If arylation of secondary sp(3) C-H bonds is desired, 8-aminoquinoline auxiliary may be used. For alkylation of sp(3) and sp(2) C-H bonds, 8-aminoquinoline auxiliary affords the best results. Some functional group tolerance is observed and amino- and hydroxy-acid derivatives can be functionalized. Preliminary mechanistic studies have been performed. A palladacycle intermediate has been isolated, characterized by X-ray crystallography, and its reactions have been studied. PMID:20175511

  12. Lung Surfactant Protein A (SP-A) Interactions with Model Lung Surfactant Lipids and an SP-B Fragment

    PubMed Central

    2011-01-01

    Surfactant protein A (SP-A) is the most abundant protein component of lung surfactant, a complex mixture of proteins and lipids. SP-A performs host defense activities and modulates the biophysical properties of surfactant in concerted action with surfactant protein B (SP-B). Current models of lung surfactant mechanism generally assume SP-A functions in its octadecameric form. However, one of the findings of this study is that when SP-A is bound to detergent and lipid micelles that mimic lung surfactant phospholipids, it exists predominantly as smaller oligomers, in sharp contrast to the much larger forms observed when alone in water. These investigations were carried out in sodium dodecyl sulfate (SDS), dodecylphosphocholine (DPC), lysomyristoylphosphatidylcholine (LMPC), lysomyristoylphosphatidylglycerol (LMPG), and mixed LMPC + LMPG micelles, using solution and diffusion nuclear magnetic resonance (NMR) spectroscopy. We have also probed SP-A’s interaction with Mini-B, a biologically active synthetic fragment of SP-B, in the presence of micelles. Despite variations in Mini-B’s own interactions with micelles of different compositions, SP-A is found to interact with Mini-B in all micelle systems and perhaps to undergo a further structural rearrangement upon interacting with Mini-B. The degree of SP-A–Mini-B interaction appears to be dependent on the type of lipid headgroup and is likely mediated through the micelles, rather than direct binding. PMID:21553841

  13. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...Navigable Waters 2 2011-07-01 2011-07-01 false Sailing Plan (SP). 161.19 Section 161.19 Navigation and...MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15...

  14. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...Navigable Waters 2 2012-07-01 2012-07-01 false Sailing Plan (SP). 161.19 Section 161.19 Navigation and...MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15...

  15. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...Navigable Waters 2 2014-07-01 2014-07-01 false Sailing Plan (SP). 161.19 Section 161.19 Navigation and...MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15...

  16. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...Navigable Waters 2 2013-07-01 2013-07-01 false Sailing Plan (SP). 161.19 Section 161.19 Navigation and...MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15...

  17. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...Navigable Waters 2 2010-07-01 2010-07-01 false Sailing Plan (SP). 161.19 Section 161.19 Navigation and...MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15...

  18. LOCALIZATION OF SP22 ON HUMAN SPERM OF DIFFERING QUALITY

    EPA Science Inventory

    LOCALIZATION OF SP22 ON HUMAN SPERM OF DIFFERING QUALITY. AE Lavers*1, GR Klinefelter2, DW Hamilton1, KP Roberts1, 1University of Minnesota, Minneapolis, MN and 2US EPA, Research Triangle Park, NC.
    SP22 is a sperm membrane protein that has been implicated in sperm function d...

  19. Field of Study Biological Sciences SOKENDAI ID SP99999

    E-print Network

    Kinosita Jr., Kazuhiko

    Field of Study Biological Sciences SOKENDAI ID SP99999 An Exploration of Invasive Species Ecology #12;Field of Study Biological Sciences SOKENDAI ID SP99999 An Exploration of Invasive Species Ecology As humans continue to alter environmental conditions throughout the world, exotic species invasions

  20. Solar Physics & Space Plasma Research Center (SP2RC)

    E-print Network

    Solar Physics & Space Plasma Research Center (SP2RC) University of SheffieldSTFC SSP Intro Summer & Statistics, The University of Sheffield (UK) http://robertus.staff.shef.ac.uk #12;Solar Physics & Space]solitons, applications) ·Conclusions #12;Solar Physics & Space Plasma Research Center (SP2RC) http

  1. Fungal Diversity Chrysoporthe doradensis sp. nov. pathogenic to Eucalyptus in

    E-print Network

    Fungal Diversity Chrysoporthe doradensis sp. nov. pathogenic to Eucalyptus in Ecuador Marieka. and Wingfield, M.J. (2005). Chrysoporthe doradensis sp. nov. pathogenic to Eucalyptus in Ecuador. Fungal Eucalyptus in various South American countries. This disease has not previously been recorded from Ecuador

  2. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Sailing Plan (SP). 161.19 Section 161.19 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY VESSEL TRAFFIC MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least...

  3. Draft Genome Sequence of Shewanella sp. Strain CP20.

    PubMed

    Lutz, Carla; Martin Tay, Qi Xiang; Sun, Shuyang; McDougald, Diane

    2015-01-01

    Shewanella sp. CP20 is a marine bacterium that survives ingestion by Tetrahymena pyriformis and is expelled from the protozoan within membrane-bound vacuoles, where the bacterial cells show long-term survival. Here, we report the draft genome sequence of Shewanella sp. CP20 and discuss the potential mechanisms facilitating intraprotozoan survival. PMID:25858840

  4. Lignin Degradation by Fusarium solani f. sp. glycines

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sudden death syndrome (SDS), caused by the soilborne fungal pathogen Fusarium solani f. sp. glycines, is one of the most important diseases of soybean. Lignin degradation may play a role in the infection, colonization, and survival of the fungus in root tissue . Lignin degradation by F. solani f. sp...

  5. Glycosylceramides from marine green microalga Tetraselmis sp.

    PubMed

    Arakaki, Atsushi; Iwama, Daisuke; Liang, Yue; Murakami, Nagisa; Ishikura, Masaharu; Tanaka, Tsuyoshi; Matsunaga, Tadashi

    2013-01-01

    Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-?3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown. PMID:23089133

  6. Meroterpenoids from a Tropical Dysidea sp. Sponge.

    PubMed

    Kim, Chang-Kwon; Woo, Jung-Kyun; Kim, Seong-Hwan; Cho, Eunji; Lee, Yeon-Ju; Lee, Hyi-Seung; Sim, Chung J; Oh, Dong-Chan; Oh, Ki-Bong; Shin, Jongheon

    2015-11-25

    Six new meroterpenoids (1-6), along with arenarol (7), a known rearranged drimane sesquiterpene hydroquinone, were isolated from a Dysidea sp. sponge collected from the Federated States of Micronesia. On the basis of the results of combined spectroscopic analysis, compound 1 was determined to be the cyclic ether derivative of 7, whereas 2 and 3 were assigned as the corresponding sesquiterpene quinones containing taurine-derived substituents. Compounds 4-6 possess a novel tetracyclic skeleton formed by a direct linkage between the quinone and sesquiterpene moieties. The configurations of these new compounds were assigned on the basis of combined NOESY and ECD analysis. These compounds exhibited cytotoxic and antimicrobial activities and weak inhibition against Na(+)/K(+)-ATPase. PMID:26551342

  7. Chitinase Production by Streptomyces sp. ANU 6277

    PubMed Central

    Narayana, Kolla J.P.; Vijayalakshmi, Muvva

    2009-01-01

    Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35°C. Culture medium amended with 1% chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield. Chitinase was purified from crude enzyme extract by single step gel filtration by Sephadex G-100. Purified chitinase of the strain exhibited a distinct protein band near 45 kDa by means of SDS-PAGE. PMID:24031419

  8. Electron microscope study of Sarcocystis sp

    USGS Publications Warehouse

    Zeve, V.H.; Price, D.L.; Herman, C.M.

    1966-01-01

    Sarcocystis sp. obtained from wild populations of grackles, Quiscalus quiscula (Linn.), were examined to clarify the effect of the parasite on the host. Electron micrographs are presented to show areas of muscle destruction adjacent to the parasite which appear to be mechanically produced by the parasite. The microtubules within the villus-like projections of the cyst suggest that their possible function is absorptive and/or conductive with regard to the production of a toxin or the conveyance of nutritive material to the developing cells. The proposed function of submembranous filaments and their relation to the conoid is discussed. Similarities in the ultrastructure to Toxoplasma and other protozoa tend to negate the relegation of Sarcocystis to the fungi and further emphasize its protozoan nature.

  9. The Behavior of Heterolepidoderma sp. (Gastrotricha).

    PubMed

    Banchetti, R; Nicola, R

    1998-01-01

    The behavior of Heterolepidoderma sp. was studied with the same approach as those already used for many species of ciliates. The ethogram we drew comprehends both helicoidal swimming (n = 20, r = 52.5 +/-12.2 mum, pitch = 512 +/- 101 mum, v--> = 215 +/- 43 mum/sec), periodically interrupted by irregular patterns changing the direction of the swimming of random angles and creeping on the substrate. The latter behavioral state, very common for the species we studied, occurs along tracks formed by successive elements (circular, C, vs linear segments, S) joined to each other by two kinds of reactions, which change their trajectory. The surprising similarities and the unexpected differences between the behavior of this gastrotrich and those of the ciliates already studied from this point of view are discussed, on the basis of the dimensional ranges and ecological niches shared by these two, definitely unrelated groups of organisms. PMID:18429665

  10. Analysis of SP-100 critical experiments

    SciTech Connect

    Sapir, J.L.; Brandon, D.I.; Collins, P.J.; Cowan, C.L.; Porter, C.A.; Andre, S.V.

    1988-01-01

    In support of the SP-100 space nuclear power source program, preliminary critical benchmark experiments were performed at the ZPPR facility at ANL-W. These configurations are representative of small, fast-spectrum, BeO-reflected, liquid metal-cooled space reactor designs at a 300-kWe power level. Analyses were performed using MCNP (Monte Carlo) and TWODANT (discrete ordinates) transport codes to calculate system criticality, control worth, and power distribution. Both methods calculated eigenvalues within 0.5% of the experimental results. Internal-poison-rod worth was underpredicted and radial reflector worth was overpredicted by both codes by up to 20%. MCNP-calculated control drum worths were underestimated by approximately 8%. Good agreement with experimental values was observed for /sup 235/U fission and for /sup 238/U fission and capture rates with the best agreement occurring in the fuel region and slightly poorer predictions apparent near BeO moderator. 7 refs., 12 figs.

  11. Cryptococcus socialis sp. nov. and Cryptococcus consortionis sp. nov., Antarctic basidioblastomycetes

    NASA Technical Reports Server (NTRS)

    Vishniac, H. S.

    1985-01-01

    New yeasts from the Ross Desert (dry valley area) of Antarctica include Cryptococcus socialis sp. nov. and Cryptococcus consortionis sp. nov. Cryptococcus socialis MYSW A801-3aY1 (= ATCC 56685) requires no vitamins, assimilates L-arabinose, cellobiose, D-glucuronate, maltose, melezitose, raffinose, soluble starch, sucrose, and trehalose, and may be distinguished from all other basidioblastomycetes by the combination of amylose production, cellobiose assimilation, and failure to utilize nitrate, D-galactose, myo-inositol, and mannitol. Its guanine-plus-cytosine content is 56 mol%. Cryptococcus consortionis MYSW A801-3aY92 (= ATCC 56686) requires thiamine, assimilates L-arabinose, D-glucuronate, 2-ketogluconate, salicin, succinate, sucrose, trehalose, and D-xylose, and may be distinguished from all other basidioblastomycetes by the combination of amylose production and failure to utilize nitrate, cellobiose, D-galactose, myo-inositol, and mannitol. Its guanine-plus-cytosine content is 56 mol%.

  12. Decidual expression and localization of human surfactant protein SP-A and SP-D, and complement protein C1q.

    PubMed

    Madhukaran, Shanmuga Priyaa; Kishore, Uday; Jamil, Kaiser; Choolani, Mahesh; Lu, Jinhua

    2015-08-01

    Surfactant proteins SP-A and SP-D, and complement protein C1q are soluble innate immune pattern recognizing molecules. SP-A, SP-D and C1q have an overall similar structure composed of an N-terminal triple-helical collagen region that is followed by a trimeric globular domain. While SP-A and SP-D belong to the collectin family (collagen containing lectin), C1q is the first recognition subcomponent of the classical pathway of the complement system. Recently, SP-A, SP-D and C1q have been considered to play important roles in early and late pregnancy. However, their expression in early human decidua has not been examined. Here, we investigated whether SP-A, SP-D and C1q are expressed within first trimester decidua in humans and their expression is associated with trophoblasts and decidual stromal cells. Decidual samples from women undergoing elective vaginal termination of pregnancy during first trimester were obtained from 25 subjects. Immunohistochemical studies using anti-human SP-A, anti-human SP-D and anti-human C1q antibodies were performed on decidual tissue sections along with anti-vimentin and cytokeratin-7 antibodies to identify stromal cells and trophoblasts. The expression was also examined by immunostaining and PCR using decidual and stromal cells. C1q expression was significantly higher when compared to SP-A and SP-D in the first trimester human decidua. Double immunostaining revealed that all stromal cells and trophoblasts expressed SP-A, SP-D and C1q, while only few invasive trophoblasts expressed C1q. Thus, expression of SP-A, SP-D and C1q in human decidua during first trimester suggests potential role of SP-A, SP-D and C1q during the early stages of pregnancy including implantation, trophoblast invasion and placental development. PMID:25829244

  13. Identification of a novel clip domain serine proteinase (Sp-cSP) and its roles in innate immune system of mud crab Scylla paramamosain.

    PubMed

    Sun, Wanwei; Li, Zhongzhen; Wang, Shasha; Wan, Weisong; Wang, Shuqi; Wen, Xiaobo; Zheng, Huaiping; Zhang, Yueling; Li, Shengkang

    2015-11-01

    Clip domain serine proteinases and their homologs are involved in the innate immunity of invertebrates. To identify the frontline defense molecules against pathogenic infection, we isolated a novel clip domain serine proteinase (Sp-cSP) from the hemocytes of mud crab Scylla paramamosain. The full-length 1362 bp Sp-cSP contains a 1155 bp open reading frame (ORF) encoding 384 amino acids. Multiple alignment analysis showed that the putative amino acid sequence of Sp-cSP has about 52% and 51% identity with Pt-cSP2 (AFA42360) and Pt-cSP3 (AFA42361) from Portunus trituberculatus, respectively, while the similarity with other cSP sequences was lower than 30%. However, all cSP sequences possess a conserved clip domain at the N-terminal and a Tryp-SPc domain at the C-terminal. The genomic organization of Sp-cSP consists of nine exons and eight introns, with some introns containing one or more tandem repeats. RT-PCR results indicated that Sp-cSP transcripts were predominantly expressed in the subcuticular epidermis, muscle and mid-intestine, but barely detectable in the brain and heart. Further, Sp-cSP transcripts were significantly up-regulated after challenge with lipopolysaccharides (LPS), Vibrio parahaemolyticus, polyinosinic polycytidylic acid (PolyI:C) or white spot syndrome virus (WSSV). Moreover, in vitro, the recombinant Sp-cSP revealed a strong antimicrobial activity against a Gram-positive (Staphylococcus aureus) and four Gram-negative (V. parahaemolyticus, Vibrio alginolyticus, Escherichia coli, Aeromonas hydrophila) bacteria in a dose-dependent manner. Taken together, the acute-phase response to immune challenges and the antimicrobial activity assay indicate that Sp-cSP is a potent immune protector and plays an important role in host defense against pathogen invasion in S. paramamosain. PMID:26272638

  14. Biocontrol of Salmonella Enteritidis in spiked chicken cuts by lytic bacteriophages ?SP-1 and ?SP-3.

    PubMed

    Augustine, Jeena; Bhat, Sarita G

    2015-04-01

    The ability of host specific bacteriophages ?SP-1 and ?SP-3 to lyse Salmonella in artificially contaminated cuts of pressure cooked chicken meat was evaluated at different temperatures -4?°C, room temperature (28?±?0.5 °C) and 37?°C applying low and high multiplicity of infection (MOI). Bacteriophages were able to significantly reduce the bacterial counts at all the temperatures studied. At 4?°C, individual application of ? SP-1 and ? SP-3 resulted in significant drop in bacterial counts (log10 2.46 and 2.1?CFU/ml, respectively) at high MOI and (log10 0.98 and 0.52?CFU/ml, respectively) at low MOI, when compared to the untreated control on day 3. Similarly at room temperature the drop was log10 3.99 and 3.46?CFU/ml at high MOI and log10 2.51 and 2.3?CFU/ml at low MOI. At 37?°C the drop was log10 1.98 and 2.38 at high MOI and at low MOI it was log10 1.52 and 1.98?CFU/ml. Increased efficiency was observed when phages where applied as cocktail at high MOI as the bacterial counts at the end of day 3 dropped by log10 3.52?CFU/ml at 37?°C and to beyond detectable level at 4?°C and room temperature. The average reduction of bacterial load in the same group was -4?°C (79%), room temperature (92%) and 37?°C (78%). PMID:25588852

  15. Haloalkylphosphorus Hydrolases Purified from Sphingomonas sp. Strain TDK1 and Sphingobium sp. Strain TCM1

    PubMed Central

    Yoshida, Satoshi; Suzuki, Yuto; Mori, Junichi; Doi, Yuka; Takahashi, Shouji; Kera, Yoshio

    2014-01-01

    Phosphotriesterases catalyze the first step of organophosphorus triester degradation. The bacterial phosphotriesterases purified and characterized to date hydrolyze mainly aryl dialkyl phosphates, such as parathion, paraoxon, and chlorpyrifos. In this study, we purified and cloned two novel phosphotriesterases from Sphingomonas sp. strain TDK1 and Sphingobium sp. strain TCM1 that hydrolyze tri(haloalkyl)phosphates, and we named these enzymes haloalkylphosphorus hydrolases (TDK-HAD and TCM-HAD, respectively). Both HADs are monomeric proteins with molecular masses of 59.6 (TDK-HAD) and 58.4 kDa (TCM-HAD). The enzyme activities were affected by the addition of divalent cations, and inductively coupled plasma mass spectrometry analysis suggested that zinc is a native cofactor for HADs. These enzymes hydrolyzed not only chlorinated organophosphates but also a brominated organophosphate [tris(2,3-dibromopropyl) phosphate], as well as triaryl phosphates (tricresyl and triphenyl phosphates). Paraoxon-methyl and paraoxon were efficiently degraded by TCM-HAD, whereas TDK-HAD showed weak activity toward these substrates. Dichlorvos was degraded only by TCM-HAD. The enzymes displayed weak or no activity against trialkyl phosphates and organophosphorothioates. The TCM-HAD and TDK-HAD genes were cloned and found to encode proteins of 583 and 574 amino acid residues, respectively. The primary structures of TCM-HAD and TDK-HAD were very similar, and the enzymes also shared sequence similarity with fenitrothion hydrolase (FedA) of Burkholderia sp. strain NF100 and organophosphorus hydrolase (OphB) of Burkholderia sp. strain JBA3. However, the substrate specificities and quaternary structures of the HADs were largely different from those of FedA and OphB. These results show that HADs from sphingomonads are novel members of the bacterial phosphotriesterase family. PMID:25038092

  16. Elevated expression and potential roles of human Sp5, a member of Sp transcription factor family, in human cancers

    SciTech Connect

    Chen Yongxin; Guo Yingqiu; Ge Xijin; Itoh, Hirotaka; Watanabe, Akira; Fujiwara, Takeshi; Kodama, Tatsuhiko; Aburatani, Hiroyuki . E-mail: haburata-tky@umin.ac.jp

    2006-02-17

    In this report, we describe the expression and function of human Sp5, a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Our experiments employing Gal4-Sp5 fusion proteins reveal multiple transcriptional domains, including a N-terminal activity domain, an intrinsic repressive element, and a C-terminal synergistic domain. Elevated expression of Sp5 was noted in several human tumors including hepatocellular carcinoma, gastric cancer, and colon cancer. To study the effects of the Sp5 protein on growth properties of human cancer cells and facilitate the identification of its downstream genes, we combined an inducible gene expression system with microarray analysis to screen for its transcriptional targets. Transfer of Sp5 into MCF-7 cells that expressed no detectable endogenous Sp5 protein elicited significant growth promotion activity. Several of the constitutively deregulated genes have been associated with tumorigenesis (CDC25C, CEACAM6, TMPRSS2, XBP1, MYBL1, ABHD2, and CXCL12) and Wnt/{beta}-Catenin signaling pathways (BAMBI, SIX1, IGFBP5, AES, and p21{sup WAF1}). This information could be utilized for further mechanistic research and for devising optimized therapeutic strategies against human cancers.

  17. Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used for perchlorate reduction by Azospira sp.

    E-print Network

    Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used was shown to be intrinsically different from the enzyme responsible for chlorate and perchlorate [(per)chlo- rate] reduction produced by Azospira sp. KJ based on subunit composition and other enzyme properties

  18. Fusarium euwallaceae sp. nov.—a symbiotic fungus of Euwallacea sp., an invasive ambrosia beetle in Israel and California

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The invasive Asian ambrosia beetle Euwallacea sp. (Coleoptera, Scolytinae, Xyleborini) and a novel Fusarium sp. that it farms in its galleries as a source of nutrition seriously damage over 20 species of live trees and pose a serious threat to avocado production (Persea americana) in Israel and Cali...

  19. Komagataella populi sp. nov. and Komagataella ulmi sp. nov., two new methanol assimilating yeasts from exudates of deciduous trees.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two new species of the methanol assimilating ascosporic yeast genus Komagataella are described. Komagataella populi sp. nov. (NRRL YB-455, CBS 12362, type strain) was isolated from an exudate on a cottonwood tree (Populus deltoides), Peoria, Illinois, USA, and Komagataella ulmi sp. nov. (NRRL YB-407...

  20. Competition between sp³-C-N vs sp³-C-F reductive elimination from Pd(IV) complexes.

    PubMed

    Pérez-Temprano, Mónica H; Racowski, Joy M; Kampf, Jeff W; Sanford, Melanie S

    2014-03-19

    This communication describes the design of a model system that allows direct investigation of competing sp(3)-C-N and sp(3)-C-F bond-forming reductive elimination from a Pd(IV) fluoro sulfonamide complex. The reductive elimination selectivity varies dramatically as a function of reaction additives. A mechanism is proposed that provides a rationale for these effects. PMID:24579653

  1. Survey of Anisakis sp. and Hysterothylacium sp. in sardines and anchovies from the North Adriatic Sea.

    PubMed

    Cavallero, S; Magnabosco, C; Civettini, M; Boffo, L; Mingarelli, G; Buratti, P; Giovanardi, O; Fortuna, C M; Arcangeli, G

    2015-05-01

    The occurrence of larval Anisakidae and Raphidascarididae in anchovies and sardines from the North Adriatic Sea has been estimated. Anisakis pegreffii and Hysterothylacium aduncum were reported, with low prevalence values. In brief, a total amount of 7650 fish specimens collected between September 2011 and 2012 were analysed using three different inspection analyses: a visual inspection of the coelomic cavity, an examination of the viscera exploiting the positive hydro-tropism of the larvae (modified Baermann technique) and enzymatic digestion of muscular tissue pools. Low level of infestation was reported for Anisakis sp. in both in anchovies and sardines, while higher values were reported for Hysterothylacium sp. Subsamples of nematodes collected were characterized at species level using the molecular diagnostic key based on ITS nuclear ribosomal region, and A. pegreffii and H. aduncum were identified. The low prevalence of Anisakis sp. in sardines and anchovies from the North Adriatic Sea could be related to the peculiar distribution of cetaceans and carnivorous zooplankton in the investigated region and could be used as a potential tag to define oily fishes from this specific fishing area as at low-risk for anisakiasis. PMID:25662709

  2. All-carbon sp-sp2 hybrid structures: Geometrical properties, current rectification, and current amplification

    PubMed Central

    Zhang, Zhenhua; Zhang, Junjun; Kwong, Gordon; Li, Ji; Fan, Zhiqiang; Deng, Xiaoqing; Tang, Guiping

    2013-01-01

    All-carbon sp-sp2 hybrid structures comprised of a zigzag-edged trigonal graphene (ZTG)and carbon chains are proposed and constructed as nanojunctions. It has been found that such simple hybrid structures possess very intriguing propertiesapp:addword:intriguing. The high-performance rectifying behaviors similar to macroscopic p-n junction diodes, such as a nearly linear positive-bias I-V curve (metallic behavior), a very small leakage current under negative bias (insulating behavior), a rather low threshold voltage, and a large bias region contributed to a rectification, can be predicted. And also, a transistor can be built by such a hybrid structure, which can show an extremely high current amplification. This is because a sp-hybrid carbon chain has a special electronic structure which can limit the electronic resonant tunneling of the ZTG to a unique and favorable situation. These results suggest that these hybrid structures might promise importantly potential applications for developing nano-scale integrated circuits. PMID:23999318

  3. New records of the Cryphonectriaceae from southern Africa including Latruncellus aurorae gen. sp. nov.

    E-print Network

    New records of the Cryphonectriaceae from southern Africa including Latruncellus aurorae gen. sp, Latruncellus aurorae gen. sp. nov., is described from Galpinia transvaalica (Lythraceae, Myrtales) in Swazi

  4. Scalability of Parallel Spatial Direct Numerical Simulations on Intel Hypercube and IBM SP1 and SP2

    NASA Technical Reports Server (NTRS)

    Joslin, Ronald D.; Hanebutte, Ulf R.; Zubair, Mohammad

    1995-01-01

    The implementation and performance of a parallel spatial direct numerical simulation (PSDNS) approach on the Intel iPSC/860 hypercube and IBM SP1 and SP2 parallel computers is documented. Spatially evolving disturbances associated with the laminar-to-turbulent transition in boundary-layer flows are computed with the PSDNS code. The feasibility of using the PSDNS to perform transition studies on these computers is examined. The results indicate that PSDNS approach can effectively be parallelized on a distributed-memory parallel machine by remapping the distributed data structure during the course of the calculation. Scalability information is provided to estimate computational costs to match the actual costs relative to changes in the number of grid points. By increasing the number of processors, slower than linear speedups are achieved with optimized (machine-dependent library) routines. This slower than linear speedup results because the computational cost is dominated by FFT routine, which yields less than ideal speedups. By using appropriate compile options and optimized library routines on the SP1, the serial code achieves 52-56 M ops on a single node of the SP1 (45 percent of theoretical peak performance). The actual performance of the PSDNS code on the SP1 is evaluated with a "real world" simulation that consists of 1.7 million grid points. One time step of this simulation is calculated on eight nodes of the SP1 in the same time as required by a Cray Y/MP supercomputer. For the same simulation, 32-nodes of the SP1 and SP2 are required to reach the performance of a Cray C-90. A 32 node SP1 (SP2) configuration is 2.9 (4.6) times faster than a Cray Y/MP for this simulation, while the hypercube is roughly 2 times slower than the Y/MP for this application. KEY WORDS: Spatial direct numerical simulations; incompressible viscous flows; spectral methods; finite differences; parallel computing.

  5. Humanized SFTPA1 and SFTPA2 Transgenic Mice Reveal Functional Divergence of SP-A1 and SP-A2

    PubMed Central

    Wang, Guirong; Guo, Xiaoxuan; DiAngelo, Susan; Thomas, Neal J.; Floros, Joanna

    2010-01-01

    Surfactant protein A (SP-A) plays a role in lung innate immunity and surfactant-related functions. Two functional genes, SP-A1 (SFTPA1) and SP-A2 (SFTPA2), are present in humans and primates (rodents have one gene). Single gene SP-A1 or SP-A2 proteins expressed in vitro are functional. To study their role in vivo, we generated humanized transgenic (hTG) C57BL/6 mice, SP-A1(6A4) and SP-A2(1A3). The SP-A cDNA in experimental constructs was driven by the 3.7-kb SP-C promoter. Positive hTG mice were bred with SP-A knock-out mice to generate F8 offspring for study. Epithelial alveolar type II cells were SP-A-positive, and Clara cells were negative by immunohistochemistry in hTG mice. The levels of SP-A in lungs of two hTG lines used were comparable with those in human lungs. Southern blot analysis indicated that two cDNA copies of either SP-A1(6A4) or SP-A2(1A3) were integrated as a concatemer into the genome of each of the two hTG lines. Electron microscopy analysis revealed that hTG mice with a single SP-A1(6A4) or SP-A2(1A3) gene product lacked tubular myelin (TM), but hTG mice carrying both had TM. Furthermore, TM was observed in human bronchoalveolar lavage fluid only if both SP-A1 and SP-A2 gene products were present and not in those containing primarily (>99.7%) either SP-A1 or SP-A2 gene products. In vivo rescue study confirmed that TM can only be restored after administering exogenous SP-A containing both SP-A1 and SP-A2 into the lungs of SP-A knock-out mice. These observations indicate that SP-A1 and SP-A2 diverged functionally at least in terms of TM formation. PMID:20048345

  6. SpIES: The Spitzer IRAC Equatorial Survey

    NASA Astrophysics Data System (ADS)

    Timlin, John; Ross, Nicholas; Richards, Gordon T.; Lacy, Mark; Bauer, Franz E.; Brandt, W. Niel; Fan, Xiaohui; Haggard, Daryl; Makler, Martin; Myers, Adam D.; Schneider, Donald P.; Strauss, Michael A.; Urry, C. Megan; Zakamska, Nadia L.; SpIES Team

    2016-01-01

    We describe the first data release from the Spitzer-IRAC Equatorial Survey (SpIES); a large-area survey of the Equatorial SDSS Stripe 82 field using Warm Spitzer. SpIES was designed to probe enough volume to perform measurements of the z>3 quasar clustering and luminosity function in order to test various "AGN feedback'' models. Additionally, the wide range of multi-wavelength, multi-epoch ancillary data makes SpIES a prime location to identify both high-redshift (z>6) quasars as well as obscured quasars missed by optical surveys. SpIES maps ~115deg2 of Stripe 82 to depths of 6.3 uJy (21.9 AB Magnitudes) and 5.75 uJy (22.0 AB Magnitudes) at [3.6] and [4.5] microns respectively; depths significantly greater than WISE. Here we define the SpIES survey parameters and describe the image processing, source extraction, and catalog production methods used to analyze the SpIES data. Amongst our preliminary science results, we show high significance detections of spectroscopically confirmed, z~5 quasars in the SpIES data. This work is based [in part] on observations made with the Spitzer Space Telescope, which is operated by the Jet Propulsion Laboratory, California Institute of Technology under a contract with NASA. Support for this work was provided by NASA through an award issued by JPL/Caltech.

  7. Mycobacterium heraklionense sp. nov.: A case series

    PubMed Central

    NEONAKIS, IOANNIS K.; SPANDIDOS, DEMETRIOS A.; GITTI, ZOE

    2015-01-01

    Mycobacterium heraklionense sp. nov. (M. heraklionense) is a novel non-tuberculous mycobacterium belonging to the Mycobacterium terrae complex that has recently been described. It has a world-wide distribution. Recently, a case of tenosynovitis in an immunocompetent individual caused by M. heraklionense was reported, indicating that it has the ability to cause diseases. In the present study, in order to provide a more detailed profile of this mycobacterium and to obtain a more complete overall picture of its clinical significance, we report all available data regarding the initial 12 cases of its isolation. Of the 12 patients, 5 (42%) eventually died within a period of 3 months following the isolation of the mycobacterium. However, any connection between the presence of M. heraklionense and these deaths could not be documented. These 5 patients were all males with a mean age of 74.6 years suffering from serious underlying diseases, which most probably were the cause of death. Additional data from possible new cases of M. heraklionense isolation are anticipated. PMID:26622497

  8. Flavisolibacter swuensis sp. nov. isolated from soil.

    PubMed

    Joo, Eun Sun; Cha, Seho; Kim, Myung Kyum; Jheong, Weonhwa; Seo, Taegun; Srinivasan, Sathiyaraj

    2015-07-01

    A Gram-staining-negative, non-motile, non-spore-forming, and rod-shaped bacterium designated as strain SR2-4-2(T) was isolated from soil in South Korea. Phylogenetic analysis based on 16S rRNA gene sequence of strain SR2-4-2(T) revealed that it belonged to the genus of Flavisolibacter, family of Chitinophagaceae, and class of Sphingobacteriia. It shared sequence similarities with Flavisolibacter ginsengisoli Gsoil 643(T) (96.4%), Flavisolibacter ginsengiterrae Gsoil 492(T) (96.3%), and Flavisolibacter rigui 02SUJ3(T) (93.0%). Chemotaxonomic data revealed that its predominant fatty acids were iso-C15:0 (26.4%) and iso-C17:0 3OH (10.7%). Its major polar lipid was phosphatidylethanolamine (PE) and its predominant respiratory quinone was MK-7. The G+C content of genomic DNA of the strain SR2-4-2(T) DNA was 45.0%. Based on the phylogenetic, chemotaxonomic, and phenotypic data, the strain SR2-4-2(T) (=JCM 19974(T) =KEMB 9004-156(T)) is classified as a type strain of a novel species for which the name of Flavisolibacter swuensis sp. nov. is proposed. PMID:26115992

  9. Pseudomonas taiwanensis sp. nov., isolated from soil.

    PubMed

    Wang, Li-Ting; Tai, Chun-Ju; Wu, Yen-Chi; Chen, Ying-Bei; Lee, Fwu-Ling; Wang, San-Lang

    2010-09-01

    A novel Gram-negative, rod-shaped, motile, non-spore-forming bacterial strain, CMS(T), isolated from soil was characterized using phenotypic and molecular taxonomic methods. 16S rRNA gene sequence analysis revealed that the organism belongs phylogenetically to the genus Pseudomonas. Pseudomonas monteilii, P. plecoglossicida and P. mosselii were the most closely related species, with 16S rRNA gene sequence similarities to the respective type strains of 99.79, 99.73 and 99.59 %. Relatively low gyrB gene sequence similarities (<90 %) and DNA-DNA reassociation values (<51 %) were obtained between the strain and its phylogenetically closest neighbours. The G+C content of strain CMS(T) was 62.7 mol%. The major cellular fatty acids were C(18 : 1) ? 7c, summed feature 3 (C(16 : 1) ? 7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(10 : 0) 3-OH. Based on the phenotypic and genetic evidence, the strain is suggested to represent a novel species, for which the name Pseudomonas taiwanensis sp. nov. is proposed. The type strain is CMS(T) (=BCRC 17751(T) =DSM 21245(T)). PMID:19854877

  10. Mineralization of phenanthrene by a Mycobacterium sp.

    PubMed Central

    Guerin, W F; Jones, G E

    1988-01-01

    A Mycobacterium sp., designated strain BG1, able to utilize the polycyclic aromatic hydrocarbon phenanthrene as the sole carbon and energy source was isolated from estuarine sediment following enrichment with the hydrocarbon. Unlike other phenanthrene degraders, this bacterium degraded phenanthrene via 1-hydroxy-2-naphthoic acid without accumulating this or other aromatic intermediates, as shown by high-performance liquid chromatography. Degradation proceeded via meta cleavage of protocatechuic acid. Different nonionic surfactants (Tween compounds) solubilized the phenanthrene to different degrees and enhanced phenanthrene utilization. The order of enhancement, however, did not correlate perfectly with increased solubility, suggesting physiological as well as physicochemical effects of the surfactants. Plasmids of approximately 21, 58, and 77 megadaltons were detected in cells grown with phenanthrene but not in those which, after growth on nutrient media, lost the phenanthrene-degrading phenotype. Given that plasmid-mediated degradations of aromatic hydrocarbons generally occur via meta cleavages, it is of interest that the addition of pyruvate, a product of meta cleavage, supported rapid mineralization of phenanthrene in broth culture; succinate, a product of ortho cleavage, supported growth but completely repressed the utilization of phenanthrene. The involvement of plasmids may have given rise to the unusual degradation pattern that was observed. Images PMID:3377503

  11. Sphingomonas humi sp. nov., isolated from soil.

    PubMed

    Yi, Tae Hoo; Han, Chang-Kyun; Srinivasan, Sathiyaraj; Lee, Kang Jin; Kim, Myung Kyum

    2010-04-01

    A gram-negative, non-motile, non-spore-forming, small, orange, rod-shaped bacterium was isolated from soil in South Korea and characterized to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequence examination revealed that strain PB323(T) belongs to the family Sphingomonadaceae. The highest degree of sequence similarity was found with Sphingomonas kaistensis PB56(T) (98.9%), followed by Sphingomonas astaxanthinifaciens TDMA-17(T) (98.3%). Chemotaxonomic characteristics (the G+C content of the genomic DNA 69.0 mol%, Q-10 quinone system, C(18:1) omega7c/omega9t/omega12t, C(16:1) omega7c/C(15:0) iso 2OH, C(17:1) omega6c, and C16:0 as the major fatty acids) corroborated assignment of strain PB323(T) to the genus Sphingomonas. Results of physiological and biochemical tests clearly demonstrate that strain PB323(T) represents a distinct species and support its affiliation with the genus Sphingomonas. Based on these data, PB323(T) (=KCTC 12341(T) =JCM 16603T =KEMB 9004-003(T)) should be classified as a type strain of a novel species, for which the name Sphingomonas humi sp. nov. is proposed. PMID:20437147

  12. Phytomonospora cypria sp. nov., isolated from soil.

    PubMed

    Sahin, Nevzat; Veyisoglu, Aysel; Tatar, Demet; Saygin, Hayrettin; Cetin, Demet; Guven, Kiymet; Klenk, Hans-Peter; Goodfellow, Michael

    2015-12-01

    A Gram-stain positive actinobacterial strain, designated KT1403(T), was isolated from a soil sample, collected from Karpaz, Magusa, Northern Cyprus, and characterised using a polyphasic approach. Morphological characteristics and chemotaxonomic data indicated that the strain belongs to the genus Phytomonospora. The cell wall of the novel strain contained meso-diaminopimelic acid and galactose, glucose and mannose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and three unidentified glycolipids. The predominant menaquinones were MK-10(H6) and MK-10(H4). The major fatty acids were found to be iso C15:0 , anteiso C15:0 and anteiso C17:0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KT1403(T) belongs to the genus Phytomonospora with a sequence similarity of 99.73 % with Phytomonospora endophytica, the type species of the genus. DNA-DNA hybridization further differentiated strain KT1403(T) from its near phylogenetic neighbour, P. endophytica DSM 45386(T) (29.0 ± 2.2 % DNA relatedness). Therefore, it is proposed that strain KT1403(T) represents a novel species of the genus Phytomonospora, for which the name Phytomonospora cypria sp. nov. is proposed. The type strain is KT1403(T) (=KCTC 29479(T) = DSM 46767(T)). PMID:26427856

  13. Lysobacter dokdonensis sp. nov., isolated from soil.

    PubMed

    Oh, Ki-Hoon; Kang, So-Jung; Jung, Yong-Taek; Oh, Tae-Kwang; Yoon, Jung-Hoon

    2011-05-01

    A Gram-negative, non-motile, rod-shaped bacterial strain, DS-58(T), was isolated from a soil sample from Dokdo, an island of Korea, and its taxonomic position was investigated. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-58(T) fell within the family Xanthomonadaceae. The isolate showed 96.9 % 16S rRNA gene sequence similarity with its closest phylogenetic neighbour, Lysobacter niastensis GH41-7(T), and 93.4-95.7 % 16S rRNA gene sequence similarity with other members of the genus Lysobacter. Strain DS-58(T) contained Q-8 as the predominant ubiquinone and iso-C(16 : 0), iso-C(15 : 0) and iso-C(17 : 1)?9c as the major fatty acids. The DNA G+C content was 68.1 mol%. Strain DS-58(T) could be distinguished phenotypically from type strains of closely related species of the genus Lysobacter and phylogenetically from all members of the genus Lysobacter. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DS-58(T) is considered to represent a novel species of the genus Lysobacter, for which the name Lysobacter dokdonensis sp. nov. is proposed. The type strain is DS-58(T) (?=?KCTC 12822(T) ?=?DSM 17958(T)). PMID:20525815

  14. Puniceibacterium sediminis sp. nov., from intertidal sediment.

    PubMed

    Zhang, De-Chao; Neuner, Kathrin; Wu, Jiang; Yao, Jianting; Margesin, Rosa

    2015-05-01

    The Gram-stain-negative, rod-shaped bacterial strain designated RU-1-R-18(T) was isolated from intertidal sediment on Sakhalin Island in Russia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RU-1-R-18(T) was related to the genus Puniceibacterium and shared highest sequence similarities with the type strain Puniceibacterium antarcticum KACC 16875(T) (97.9%). The predominant cellular fatty acid was C(18:1)?7c. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified aminophospholipid and seven unidentified polar lipids. The genomic DNA G+C content of strain RU-1-R-18(T) was 59.1 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain RU-1-R-18(T) represents a novel species of the genus Puniceibacterium , for which the name Puniceibacterium sediminis sp. nov. is proposed (type strain RU-1-R-18(T)?=LMG 28384(T)?=DSM 29052(T)). PMID:25687347

  15. Systematic Enumeration of sp(3) Nanothreads.

    PubMed

    Xu, En-shi; Lammert, Paul E; Crespi, Vincent H

    2015-08-12

    Slow decompression of crystalline benzene in large-volume high-pressure cells has recently achieved synthesis of a novel one-dimensional allotrope of sp(3) carbon in which stacked columns of benzene molecules rehybridize into an ordered crystal of nanothreads. The progenitor benzene molecules function as six-valent one-dimensional superatoms with multiple binding sites. Here we enumerate their hexavalent bonding geometries, recognizing that the repeat unit of interatomic connectivity ("topological unit cell") need not coincide with the crystallographic unit cell, and identify the most energetically favorable cases. A topological unit cell of one or two benzene rings with at least two bonds interconnecting each adjacent pair of rings, accommodates 50 topologically distinct nanothreads, 15 of which are within 80 meV/carbon atom of the most stable member. Optimization of aperiodic helicity reveals the most stable structures to be chiral. We generalize Euler's rules for ring counting to cover this new form of very thin one-dimensional carbon, calculated their physical properties, and propose a naming convention that can be generalized to handle nanothreads formed from other progenitor molecules. PMID:26207926

  16. Preliminary SP-100/Stirling heat exchanger designs

    NASA Technical Reports Server (NTRS)

    Schmitz, Paul; Tower, Leonard; Dawson, Ronald; Blue, Brian; Dunn, Pat

    1993-01-01

    Analytic modeling of several heat exchanger concepts to couple the SP-100 nuclear reactor primary lithium loop and the Space Stirling Power Convertor (SSPC) was performed. Four 25 kWe SSPC's are used to produce the required 100 kW of electrical power. This design work focused on the interface between a single SSPC and the primary lithium loop. Manifolding to separate and collect the four channel flow was not modeled. This work modeled two separate types of heat exchanger interfaces (conductive coupling and radiative coupling) to explore their relative advantages and disadvantages. The minimum mass design of the conductively coupled concepts was 18 kg or 0.73 kg/kWe for a single 25 kWe convertor. The minimum mass radiatively coupled concept was 41 kg or 1.64 kg/kWe. The direct conduction heat exchanger provides a lighter weight system because of its ability to operate the Stirling convertor evaporator at higher heat fluxes than those attainable by the radiatively coupled systems. Additionally the conductively coupled concepts had relatively small volumes and provide potentially simpler assembly. Their disadvantages were the tight tolerances and material joining problems associated with this refractory to superalloy interface. The advantages of the radiatively coupled designs were the minimal material interface problems.

  17. Floating assembly of diatom Coscinodiscus sp. microshells.

    PubMed

    Wang, Yu; Pan, Junfeng; Cai, Jun; Zhang, Deyuan

    2012-03-30

    Diatoms have silica frustules with transparent and delicate micro/nano scale structures, two dimensional pore arrays, and large surface areas. Although, the diatom cells of Coscinodiscus sp. live underwater, we found that their valves can float on water and assemble together. Experiments show that the convex shape and the 40 nm sieve pores of the valves allow them to float on water, and that the buoyancy and the micro-range attractive forces cause the valves to assemble together at the highest point of water. As measured by AFM calibrated glass needles fixed in manipulator, the buoyancy force on a single floating valve may reach up to 10 ?N in water. Turning the valves over, enlarging the sieve pores, reducing the surface tension of water, or vacuum pumping may cause the floating valves to sink. After the water has evaporated, the floating valves remained in their assembled state and formed a monolayer film. The bonded diatom monolayer may be valuable in studies on diatom based optical devices, biosensors, solar cells, and batteries, to better use the optical and adsorption properties of frustules. The floating assembly phenomenon can also be used as a self-assembly method for fabricating monolayer of circular plates. PMID:22387476

  18. Gryllotalpicola soli sp. nov., isolated from soil.

    PubMed

    Moon, Ji-Young; Kim, Soo-Jin; Hamada, Moriyuki; Ahn, Jae-Hyung; Weon, Hang-Yeon; Suzuki, Ken-ichiro; Yoon, Jung-Hoon; Kwon, Soon-Wo

    2014-12-01

    A novel Gram-stain-positive, short rod-shaped, non-flagellated and mesophilic strain, KIS12-7(T), isolated from a soil sample collected from Daecheong-Island in Ongjin County, Republic of Korea, was studied using a polyphasic approach. Phylogenetic trees based on the 16S rRNA gene sequence revealed that the novel strain was a member of the genus Gryllotalpicola, showing more than 97.0?% sequence similarity with Gryllotalpicola daejeonensis RU-04(T) (98.0?%), Gryllotalpicola koreensis RU-16(T) (97.7?%) and Gryllotalpicola kribbensis PU-02(T) (97.3?%). However, DNA-DNA relatedness values demonstrated that strain KIS12-7(T) could be clearly distinguished from closely related species of the genus Gryllotalpicola. The cell-wall peptidoglycan of strain KIS12-7(T) was of the type B2 and the acyl type was acetyl. The predominant menaquinones were MK-11 and MK-10. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown phosphoglycolipid, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The G+C content of the genomic DNA was 72.1 mol%. On the basis of the evidence presented, strain KIS12-7(T) is a representative of a novel species of the genus Gryllotalpicola, and the name Gryllotalpicola soli sp. nov. is proposed; the type strain is KIS12-7(T) (?=?DSM 27182(T)?=?KACC 17302(T)?=?NBRC 109659(T)). PMID:25240021

  19. An Acetylenic Alkaloid from the Calcareous Sponge Leucetta sp.

    PubMed Central

    Hermawan, Idam; de Voogd, Nicole J.; Tanaka, Junichi

    2011-01-01

    A new acetylenic alkaloid was isolated from the sponge Leucetta sp. The structure was established by analyzing spectroscopic data. The alkaloid showed cytotoxicity IC50 2.5 ?g/mL against NBT-T2 cells. PMID:21556167

  20. Users guide for the ANL IBM SP1

    SciTech Connect

    Gropp, W.; Lusk, E.; Pieper, S.C.

    1994-10-01

    This guide presents the features of the IBM SP1 installed in the Mathematics and Computer Science Division at Argonne National Laboratory. The guide describes the available hardware and software, access policies, and hints for using the system productively.

  1. The complete mitochondrial genome of Odontesthes sp. (Atheriniforms, Atherinopsidae).

    PubMed

    Wei, Tao; Shi, Ge; Wang, Rixin

    2015-12-01

    In this study, the complete mitochondrial genome of Odontesthes sp. has been studied first. The genome is 16,528?bp in length and contains 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes and 1 putative control region. The mitochondrial genome of Odontesthes sp. had common features about gene arrangement and tRNA structures compared with those of other teleost fishes. The overall base composition of Odontesthes sp. is T 27.3%, C 29.4%, A 26.0% and G 17.3%, with a slight A?+?T bias of 53.3%. Meanwhile, the conserved motif 5'-GCCGG-3' was determined in the origin of light-strand replication of Odontesthes sp. The mitochondrial genome of this species would play an important role in the phylogenetics of Atherinopsidae. PMID:24438260

  2. Launch vehicle integration requirements for SP-100. Technical information report

    SciTech Connect

    Shaw, L.T. Jr.; Womack, J.R.

    1984-03-01

    SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS) Space Shuttle System into a near-earth orbit, deployed, and through upper stage propulsion burn(s) be inserted/transferred to its mission orbit. The nature of the advanced design SP-100 payloads using this power plant are physically and functionally compatible with the NSTS and meet the safety requirements thereof. The purpose of this document is to define and present the requirements and interface provisions that, when satisfied, will ensure technical compatibility between SP-100 systems and the NSTS.

  3. 'PROCTOECES' SP. (TREMATODA: DIGENEA) IN THE AMERICAN OYSTER, 'CRASSOSTREA VIRGINICA'

    EPA Science Inventory

    Histological examination of over 6,000 oysters, Crassostrea virginica, inhabiting northern Gulf Coast estuaries revealed unencysted junvenile and possible adult stages of digenetic trematode, Proctoeces sp., inhabiting the gonadal ducts of the mollusc. The morphology of the worm ...

  4. Environmental interactions and the SP-100 power system

    SciTech Connect

    Ferguson, D.C.

    1993-01-01

    The Civil Space Technology Initiative High-Capacity-Power Environmental Interactions Program has made great progress in defining and evaluating the interactions of the SP-100 power system with its expected ambient environments. The NASCAP/LEO and POLAR computer codes demonstrated that local electric fields at the user interface module are high. Therefore, particular attention must be paid to geometries and materials in this region to prevent arcing at conductor-insulator junctions in low Earth orbit. NASCAP/LEO and EPSAT computer models revealed that SP-100 payloads float about 100 V negative of the LEO plasma. In addition, ground tests and modeling done for the Space Station Freedom Electrical Grounding Tiger Team found that dielectric coatings often break down at such voltages in a plasma. Thus, surface coatings for SP-100 payloads should be carefully selected. Sputtering may also be a concern for long-duration missions in LEO at these voltages. Much work has been done on a sputtering model to evaluate surface material loss rates on SP-100 payloads. In ground plasma chamber tests of cables and cable insulators at SP-100 voltages, parasitic power losses due to the plasma current collected from possible pinholes or coating defects were quantified and shown to be small. Modeling revealed that the power loss from currents to other surfaces is also small. The atomic oxygen durability of SP-100 materials and coatings continues to be investigated in ground tests. In the upcoming Evaluation of Oxygen Interaction with Materials (EOIM-3) Shuttle flight experiment, a host of SP-100 materials will be evaluated for atomic oxygen durability in LEO. Finally, an evaluation of the interactions of the SP-100 power system with lunar and planetary environments has started. At a workshop on chemical and electrical interactions on Mars recently held at the NASA Lewis Research Center, many of primary interactions were identified.

  5. Genome sequencing and annotation of Serratia sp. strain TEL.

    PubMed

    Lephoto, Tiisetso E; Gray, Vincent M

    2015-12-01

    We present the annotation of the draft genome sequence of Serratia sp. strain TEL (GenBank accession number KP711410). This organism was isolated from entomopathogenic nematode Oscheius sp. strain TEL (GenBank accession number KM492926) collected from grassland soil and has a genome size of 5,000,541 bp and 542 subsystems. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession number LDEG00000000. PMID:26697332

  6. Vanadium-Catalyzed C(sp3 )H Fluorination Reactions

    E-print Network

    Chen, Chuo

    S1 Vanadium-Catalyzed C(sp3 )­H Fluorination Reactions Ji-Bao Xia, Yuyong Ma, and Chuo Chen. Vanadium(III) Oxide (95%) was purchased from Strem Chemicals Inc. Fluorobenzene (99.5%) was purchased from procedure for the V2O3-catalyzed C(sp3 )­H fluorination reaction. To a 4 mL clear vial charged with vanadium

  7. Genome sequencing and annotation of Serratia sp. strain TEL

    PubMed Central

    Lephoto, Tiisetso E.; Gray, Vincent M.

    2015-01-01

    We present the annotation of the draft genome sequence of Serratia sp. strain TEL (GenBank accession number KP711410). This organism was isolated from entomopathogenic nematode Oscheius sp. strain TEL (GenBank accession number KM492926) collected from grassland soil and has a genome size of 5,000,541 bp and 542 subsystems. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession number LDEG00000000. PMID:26697332

  8. Eustrongylides sp. epizootic in young common egrets (Casmerodius albus)

    USGS Publications Warehouse

    Roffe, Thomas J.

    1988-01-01

    In May 1985, epizootic mortality was reported in common egrets (Casmerodius albus) on Avery Island, Louisiana. Subsequent investigation revealed that more than 400 birds died. Severe peritoneal nematodiasis (Eustrongylides sp.) was found on postmortem examination. A nearby breeding rookery on the same island was apparently unaffected. Reasons for this selective mortality are presented. Three other reports of epizootic mortality due to Eustrongylides sp. have been reported. This is the first report of this type of epizootic in gulf-coast birds.

  9. Sp2 DNA Binding Activity and trans-Activation Are Negatively Regulated in Mammalian Cells*

    E-print Network

    Horowitz, Jonathan M.

    Sp2 DNA Binding Activity and trans-Activation Are Negatively Regulated in Mammalian Cells* Received of Sp2-binding sites within the DHFR promoter increased Sp2-mediated trans-activation in transient co-transfection experiments but also revealed Sp2 to be a relatively weak trans-activator with little or no capacity

  10. Chapter 1. Introduction 1 CRiSP Harvest Chapter 1. Introduction

    E-print Network

    Washington at Seattle, University of

    Chapter 1. Introduction 1 CRiSP Harvest Chapter 1. Introduction 1 1 1 Table of Contents 1............................................................................................3 1.3 - Brief History of the PSC Chinook and CRiSP Harvest Models .....................7 1.4 - CRiSP #12;Chapter 1. Introduction 2 CRiSP Harvest 1.1 - Welcome Welcome to the Columbia River Salmon Passage

  11. Total Dissolved Gas submodel parameter calibration for use with CRiSP

    E-print Network

    Washington at Seattle, University of

    Total Dissolved Gas submodel parameter calibration for use with CRiSP 7/10/2003 3:08 PM W. Nicholas in CriSP at mid season 2003. New methods are described in italics like this. Beginning in 1999, CRiSP into spill water. At that time, various parameters were calibrated and made available for use in the CRiSP

  12. Dissolved Gas in the Snake and Columbia Rivers Modeled by CRiSP

    E-print Network

    Washington at Seattle, University of

    Dissolved Gas in the Snake and Columbia Rivers 1969-1984 Modeled by CRiSP Pamela Shaw Columbia created using CRiSP and historic spill and flow data. In CRiSP the gas going into the tailwater. The gas production equations in CRiSP were fit to gas data from 1995-1998 and so should only be used

  13. Three new Scandinavian species of Culicoides (Culicoides): Culicoides boyi sp. nov., Culicoides selandicus sp. nov. and Culicoides kalix sp. nov. (Diptera: Ceratopogonidae)

    PubMed Central

    Kristensen, Michael; Pape, Thomas

    2015-01-01

    Abstract Background In the context of a major monitoring program of Culicoides in Denmark and Sweden due to the appearance of bluetongue disease in 2007–2008, a large number of specimens were collected by light traps and sorted morphologically, with COI barcodes generated for selected specimens. New information Three species are described as new to science based on both morphological and molecular data: Culicoides (Culicoides) boyi sp. nov. (Denmark: Jutland), C. (C.) selandicus sp. nov. (Denmark: Zealand) and C. (C.) kalix sp. nov. (Sweden: Norrbotten). All are diagnosed morphologically as well as by molecular barcoding. A key to slide-mounted females of all Scandinavian species of Culicoides (Culicoides) is presented. PMID:26696760

  14. An antibody against the surfactant protein A (SP-A)-binding domain of the SP-A receptor inhibits T cell-mediated immune responses to Mycobacterium tuberculosis

    PubMed Central

    Samten, Buka; Townsend, James C.; Sever-Chroneos, Zvjezdana; Pasquinelli, Virginia; Barnes, Peter F.; Chroneos, Zissis C.

    2008-01-01

    Surfactant protein A (SP-A) suppresses lymphocyte proliferation and IL-2 secretion, in part, by binding to its receptor, SP-R210. However, the mechanisms underlying this effect are not well understood. Here, we studied the effect of antibodies against the SP-A-binding (neck) domain (?-SP-R210n) or nonbinding C-terminal domain (?-SP-R210ct) of SP-R210 on human peripheral blood T cell immune responses against Mycobacterium tuberculosis. We demonstrated that both antibodies bind to more than 90% of monocytes and 5–10% of CD3+ T cells in freshly isolated PBMC. Stimulation of PBMC from healthy tuberculin reactors [purified protein derivative-positive (PPD+)] with heat-killed M. tuberculosis induced increased antibody binding to CD3+ cells. Increased antibody binding suggested enhanced expression of SP-R210, and this was confirmed by Western blotting. The antibodies (?-SP-R210n) cross-linking the SP-R210 through the SP-A-binding domain markedly inhibited cell proliferation and IFN-? secretion by PBMC from PPD+ donors in response to heat-killed M. tuberculosis, whereas preimmune IgG and antibodies (?-SP-R210ct) cross-linking SP-R210 through the non-SP-A-binding, C-terminal domain had no effect. Anti-SP-R210n also decreased M. tuberculosis-induced production of TNF-? but increased production of IL-10. Inhibition of IFN-? production by ?-SP-R210n was abrogated by the combination of neutralizing antibodies to IL-10 and TGF-?1. Together, these findings support the hypothesis that SP-A, via SP-R210, suppresses cell-mediated immunity against M. tuberculosis via a mechanism that up-regulates secretion of IL-10 and TGF-?1. PMID:18443188

  15. Co-infection of the Siberian hamster (Phodopus sungorus) with a novel Helicobacter sp. and Campylobacter sp.

    PubMed

    Nagamine, Claude M; Shen, Zeli; Luong, Richard H; McKeon, Gabriel P; Ruby, Norman F; Fox, James G

    2015-05-01

    We report the isolation of a novel helicobacter isolated from the caecum of the Siberian hamster (Phodopus sungorus). Sequence analysis showed 97% sequence similarity to Helicobacter ganmani. In addition, we report the co-infection of these Siberian hamsters with a Campylobacter sp. and a second Helicobacter sp. with 99% sequence similarity to Helicobacter sp. flexispira taxon 8 (Helicobacter bilis), a species isolated previously from patients with bacteraemia. Gross necropsy and histopathology did not reveal any overt pathological lesions of the liver and gastrointestinal tract that could be attributed to the Helicobacter or Campylobacter spp. infections. This is the first helicobacter to be identified in the Siberian hamster and the first report of co-infection of Helicobacter spp. and Campylobacter sp. in asymptomatic Siberian hamsters. PMID:25752854

  16. Co-infection of the Siberian hamster (Phodopus sungorus) with a novel Helicobacter sp. and Campylobacter sp.

    PubMed Central

    Shen, Zeli; Luong, Richard H.; McKeon, Gabriel P.; Ruby, Norman F.; Fox, James G.

    2015-01-01

    We report the isolation of a novel helicobacter isolated from the caecum of the Siberian hamster (Phodopus sungorus). Sequence analysis showed 97?% sequence similarity to Helicobacter ganmani. In addition, we report the co-infection of these Siberian hamsters with a Campylobacter sp. and a second Helicobacter sp. with 99?% sequence similarity to Helicobacter sp. flexispira taxon 8 (Helicobacter bilis), a species isolated previously from patients with bacteraemia. Gross necropsy and histopathology did not reveal any overt pathological lesions of the liver and gastrointestinal tract that could be attributed to the Helicobacter or Campylobacter spp. infections. This is the first helicobacter to be identified in the Siberian hamster and the first report of co-infection of Helicobacter spp. and Campylobacter sp. in asymptomatic Siberian hamsters. PMID:25752854

  17. sp(2)-sp(3) diboranes: astounding structural variability and mild sources of nucleophilic boron for organic synthesis.

    PubMed

    Dewhurst, Rian D; Neeve, Emily C; Braunschweig, Holger; Marder, Todd B

    2015-06-14

    Despite the widespread use of organoborane reagents in organic synthesis and catalysis, a major challenge still remains: very few boron-centered nucleophiles exist for the direct construction of B-C bonds. Perhaps the most promising emerging solution to this problem is the use of sp(2)-sp(3) diboranes, in which one boron atom of a conventional diborane(4) is quaternised by either a neutral or anionic nucleophile. These compounds, either isolated or generated in situ, serve as relatively mild and convenient sources of the boryl anion [BR2](-) for use in organic synthesis and have already proven their efficacy in metal-free as well as metal-catalysed borylation reactions. This Feature article documents the history of sp(2)-sp(3) diborane synthesis, their properties and surprising structural variability, and their burgeoning utility in organic synthesis. PMID:25912808

  18. Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan.

    PubMed

    Alperi, Anabel; Martínez-Murcia, Antonio J; Ko, Wen-Chien; Monera, Arturo; Saavedra, Maria J; Figueras, Maria J

    2010-09-01

    Two clinical Aeromonas strains (A2-50(T) and A2-67(T)) recovered from the wounds of two patients in Taiwan could not be assigned to any known species of this genus based on their 16S rRNA gene sequences, which showed similarities of 99.6-99.8 % to those of the type strains of Aeromonas caviae, A. trota and A. aquariorum. The rpoD phylogenetic tree allocated these strains to two novel and independent phylogenetic lines, the neighbouring species being A. caviae, the type strain of which showed 93.2 % similarity (56 bp differences) to strain A2-50(T) and 92.2 % (63 bp differences) to strain A2-67(T). A multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) confirmed that the two strains formed independent phylogenetic lineages within the genus. These data, together with phenotypic characterization and DNA-DNA reassociation results, revealed that these strains represent novel Aeromonas species, for which the names Aeromonas taiwanensis sp. nov. (type strain A2-50(T) =CECT 7403(T) =LMG 24683(T)) and Aeromonas sanarellii sp. nov. (type strain A2-67(T) =CECT 7402(T) =LMG 24682(T)) are proposed. PMID:19819994

  19. Characterization of a Bioflocculant Produced by a Consortium of Halomonas sp. Okoh and Micrococcus sp. Leo

    PubMed Central

    Okaiyeto, Kunle; Nwodo, Uchechukwu U.; Mabinya, Leonard V.; Okoh, Anthony I.

    2013-01-01

    The physicochemical and flocculating properties of a bioflocculant produced by a bacterial consortium composed of Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The purified bioflocculant was cation and pH dependent, and optimally flocculated kaolin clay suspension at a dosage of 0.1 mg/mL. The flocculating activity of the bioflocculant was stimulated in the presence of Ca2+, Mn2+, Al3+ and had a wide pH range of 2–10, with the highest flocculating activity of 86% at pH 8. The bioflocculant was thermostable and retained more than 70% of its flocculating activity after being heated at 80 °C for 30 min. Thermogravimetric analyses revealed a partial thermal decomposition of the biofloculant at 400 °C. The infrared spectrum showed the presence of hydroxyl, carboxyl and amino moieties as functional groups. The bioflocculant produced by the bacterial consortium appears to hold promising alternative to inorganic and synthetic organic flocculants that are widely used in wastewater treatment. PMID:24135818

  20. Enteractinococcus lamae sp. nov. and Enteractinococcus viverrae sp. nov., isolated from animal faeces.

    PubMed

    Chen, Xiu; Li, Gui-Ding; Li, Qin-Yuan; Hu, Cai-Juan; Qiu, Shu-Mei; Jiang, Yi; Jiang, Cheng-Lin; Han, Li; Huang, Xue-Shi

    2015-12-01

    Two novel actinobacteria, designated strains YIM 101617(T) and YIM 101632(T), were isolated from Lama pacos (alpaca) and Viverra zibetha (civet) faeces in Yunnan Wild Animal Park in Yunnan province, southwestern China. Both strains should be placed in genus Enteractinococcus based on phylogenetic analysis. Based on 16S rRNA gene sequence analysis, strain YIM 101617(T) exhibits high similarity to Enteractinococcus fodinae DSM 22966(T) (97.70 %) and Enteractinococcus coprophilus YIM 100590(T) (97.45 %), whilst YIM 101632(T) exhibits high similarity to Enteractinococcus coprophilus YIM 100590(T) (97.25 %), and the similarity between YIM 101617(T) and YIM 101632(T) is 95.90 %. However, DNA-DNA hybridization values of the two strains with the type strains in the genus Enteractinococcus were low (<70 %). Most morphological and chemotaxonomic characteristics of the two strains were found to be similar to those of species in the genus Enteractinococcus but also some differences were observed. The DNA G+C contents of strains YIM 101617(T) and YIM 101632(T) were determined to be 55.9 and 56.4 mol%, respectively. Based on these data, the two strains are concluded to represent two different novel species in the genus Enteractinococcus. The names Enteractinococcus lamae sp. nov. (type strain YIM 101617(T)=DSM 27612(T)=CCTCC AB 2013230(T)) and Enteractinococcus viverrae sp. nov. (type strain YIM 101632(T)=KCTC 39552(T)=CCTCC AB 2013280(T)) are proposed, respectively. PMID:26423082

  1. Aminobacter ciceronei sp. nov. and Aminobacter lissarensis sp. nov., isolated from various terrestrial environments

    USGS Publications Warehouse

    McDonald, I.R.; Kampfer, P.; Topp, E.; Warner, K.L.; Cox, M.J.; Connell, Hancock T.L.; Miller, L.G.; Larkin, M.J.; Ducrocq, V.; Coulter, C.; Harper, D.B.; Murrell, J.C.; Oremland, R.S.

    2005-01-01

    The bacterial strains IMB-1T and CC495T, which are capable of growth on methyl chloride (CH3Cl, chloromethane) and methyl bromide (CH3Br, bromomethane), were isolated from agricultural soil in California fumigated with CH3Br, and woodland soil in Northern Ireland, respectively. Two pesticide- /herbicide-degrading bacteria, strains ER2 and C147, were isolated from agricultural soil in Canada. Strain ER2 degrades N-methyl carbamate insecticides, and strain C147 degrades triazine herbicides widely used in agriculture. On the basis of their morphological, physiological and genotypic characteristics, these four strains are considered to represent two novel species of the genus Aminobacter, for which the names Aminobacter ciceronei sp. nov. (type strain IMB-1T=ATCC 202197T=CIP 108660T=CCUG 50580T; strains ER2 and C147) and Aminobacter lissarensis sp. nov. (type strain CC495T=NCIMB 13798T=CIP 108661T=CCUG 50579T) are proposed. ?? 2005 IUMS.

  2. Microsphaerol and seimatorone: two new compounds isolated from the endophytic fungi, Microsphaeropsis sp. and Seimatosporium sp.

    PubMed

    Hussain, Hidayat; Root, Natalia; Jabeen, Farah; Al-Harrasi, Ahmed; Ahmad, Manzoor; Mabood, Fazal; Hassan, Zahid; Shah, Afzal; Green, Ivan R; Schulz, Barbara; Krohn, Karsten

    2015-02-01

    A new polychlorinated triphenyl diether named microsphaerol (1), has been isolated from the endophtic fungus Microsphaeropsis sp. An intensive phytochemical investigation of the endophytic fungus Seimatosporium sp., led to the isolation of a new naphthalene derivative named seimatorone (2) and eight known compounds, i.e., 1-(2,6-dihydroxyphenyl)-3-hydroxybutan-1-one (3), 1-(2,6-dihydroxyphenyl)butan-1-one (4), 1-(2-hydroxy-6-methoxyphenyl)butan-1-one (5), 5-hydroxy-2-methyl-4H-chromen-4-one (6), 2,3-dihydro-5-hydroxy-2-methyl-4H-chromen-4-one (7), 8-methoxynaphthalen-1-ol (8), nodulisporins A and B (9 and 10, resp.), and daldinol (11). The structures of 1 and 2 were elucidated by detailed spectroscopic analysis including (1) H- and (13) C-NMR, COSY, HMQC, HMBC, and HR-EI-MS, while the structures of the known compounds were deduced from comparison of their spectral data with those in the literature. Preliminary studies revealed that microsphaerol (1) showed good antibacterial activities against B. Megaterium and E. coli, and good antilagal and antifungal activities against C. fusca, M. violaceum, respectively. On the other hand, seimatorone (2) exhibited moderate antibacterial, antialgal, and antifungal activities. PMID:25676510

  3. [Purification and physico-chemical properties of Streptomyces sp. 1349 collagenase and Streptomyces sp. 1382 keratinase].

    PubMed

    Ivanko, O V; Varbanets', L D

    2004-01-01

    The schemes of isolation and purification of collagenolytic enzymes of Streptomyces sp. 1349 and keratinolyte enzymes of Streptomyces sp. 1382, which include fractionation by ammonium sulphate separation on TSK-gels: ion-exchange chromatography on Toyopearl DEAE-650(M) and gel-filtration on Toyopearl HW-50, as well as highly efficient liquid chromatography. The purified enzyme preparations proved to be proteases of serine type (collagenase 2 and keratinases) as well as metalloproteases (collagenases 1 and 3). It has seen established that collagenases are enzymes of broad specificity, which are active in respect of proteins of both globular and fibrillar nature. And vice versa, keratinases are proteolytic enzymes of narrow specificity which hydrolyze native keratin. Molecular masses of purified enzyme preparations, from the data of SDS-PAAG are approximately 30-40 kDa (collagenases 1-3) and about 15-20 kDa (keratinases 1 and 2). It is shown that the charged aminoacid residues (about 85%) prevail in enzyme molecules. The enzymes are distinguished by pH- and thermooptima. PMID:15208850

  4. Vibrio neonatus sp. nov. and Vibrio ezurae sp. nov. isolated from the gut of Japanese abalones.

    PubMed

    Sawabe, Tomoo; Hayashi, Karin; Moriwaki, Jun; Fukui, Youhei; Thompson, Fabiano L; Swings, Jean; Christen, Richard

    2004-09-01

    Five alginolytic, facultative anaerobic, non-motile bacteria were isolated from the gut of Japanese abalones (Haliotis discus discus, H. diversicolor diversicolor and H. diversicolor aquatilis). Phylogenetic analyses based on 16S rRNA gene and gap gene sequences indicated that these strains are closely related to V. halioticoli. DNA-DNA hybridizations, FAFLP fingerprintings, and phylogenies of gap and 16S rRNA gene sequences showed that the five strains represent two species different from all currently described vibrios. The names Vibrio neonatus sp. nov. (IAM 15060T = LMG 19973T = HDD3-1T; mol% G+C of DNA is 42.1-43.9), and Vibrio ezurae sp. nov. (IAM 15061T = LMG 19970T = HDS1-1T; mol% G+C of DNA is 43.6-44.8) are proposed to encompass these new taxa. The two new species can be differentiated from V. halioticoli on the basis of several features, including beta-galactosidase activity, assimilation of glycerol, D-mannose and D-gluconate. PMID:15490553

  5. Listeria booriae sp. nov. and Listeria newyorkensis sp. nov., from food processing environments in the USA.

    PubMed

    Weller, Daniel; Andrus, Alexis; Wiedmann, Martin; den Bakker, Henk C

    2015-01-01

    Sampling of seafood and dairy processing facilities in the north-eastern USA produced 18 isolates of Listeria spp. that could not be identified at the species-level using traditional phenotypic and genotypic identification methods. Results of phenotypic and genotypic analyses suggested that the isolates represent two novel species with an average nucleotide blast identity of less than 92% with previously described species of the genus Listeria. Phylogenetic analyses based on whole genome sequences, 16S rRNA gene and sigB gene sequences confirmed that the isolates represented by type strain FSL M6-0635(T) and FSL A5-0209 cluster phylogenetically with Listeria cornellensis. Phylogenetic analyses also showed that the isolates represented by type strain FSL A5-0281(T) cluster phylogenetically with Listeria riparia. The name Listeria booriae sp. nov. is proposed for the species represented by type strain FSL A5-0281(T) (?=DSM 28860(T)?=LMG 28311(T)), and the name Listeria newyorkensis sp. nov. is proposed for the species represented by type strain FSL M6-0635(T) (?=DSM 28861(T)?=LMG 28310(T)). Phenotypic and genotypic analyses suggest that neither species is pathogenic. PMID:25342111

  6. Streptococcus loxodontisalivarius sp. nov. and Streptococcus saliviloxodontae sp. nov., isolated from oral cavities of elephants.

    PubMed

    Saito, Masanori; Shinozaki-Kuwahara, Noriko; Hirasawa, Masatomo; Takada, Kazuko

    2014-09-01

    Four Gram-stain-positive, catalase-negative, coccoid-shaped organisms were isolated from elephant oral cavities. The isolates were tentatively identified as streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene sequencing studies confirmed the organisms to be members of the genus Streptococcus. Two isolates (NUM 6304(T) and NUM 6312) were related most closely to Streptococcus salivarius with 96.8?% and 93.1?% similarity based on the 16S rRNA gene and the RNA polymerase ? subunit encoding gene (rpoB), respectively, and to Streptococcus vestibularis with 83.7?% similarity based on the 60 kDa heat-shock protein gene (groEL). The other two isolates (NUM 6306(T) and NUM 6318) were related most closely to S. vestibularis with 97.0?% and 82.9?% similarity based on the 16S rRNA and groEL genes, respectively, and to S. salivarius with 93.5?% similarity based on the rpoB gene. Based on phylogenetic and phenotypic evidence, these isolates are suggested to represent novel species of the genus Streptococcus, for which the names Streptococcus loxodontisalivarius sp. nov. (type strain NUM 6304(T)?=?JCM 19287(T)?=?DSM 27382(T)) and Streptococcus saliviloxodontae sp. nov. (type strain NUM 6306(T)?=?JCM 19288(T)?=?DSM 27513(T)) are proposed. PMID:24994777

  7. Novosphingobium marinum sp. nov., isolated from seawater.

    PubMed

    Huo, Ying-Yi; You, Hong; Li, Zheng-Yang; Wang, Chun-Sheng; Xu, Xue-Wei

    2015-02-01

    A Gram-stain-negative, aerobic, short rod-shaped bacterium, strain LA53(T), was isolated from a deep-sea water sample collected from the eastern Pacific Ocean. Strain LA53(T) grew in the presence of 0-7.0 % (w/v) NaCl and at 15-37 °C; optimum growth was observed with 1.0-2.0 % (w/v) NaCl and at 35 °C. Chemotaxonomic analysis showed ubiquinone-10 as the predominant respiratory quinone, C18 : 1?7c and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1?7c) as major fatty acids, and diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid as major polar lipids. The genomic DNA G+C content was 57.7 mol%. Phylogenetic analyses revealed that strain LA53(T) belongs to the genus Novosphingobium. 16S rRNA gene sequence similarities between strain LA53(T) and the type strains of species of the genus Novosphingobium with validly published names ranged from 93.1 to 96.3 %. In addition, strain LA53(T) could be differentiated from Novosphingobium pentaromativorans DSM 17173(T) and Novosphingobium indicum DSM 23608(T) as well as the type strain of the type species of the genus, Novosphingobium capsulatum DSM 30196(T), by some phenotypic characteristics, including hydrolysis of substrates, utilization of carbon sources and susceptibility to antibiotics. On the basis of phenotypic and genotypic data, strain LA53(T) represents a novel species within the genus Novosphingobium, for which the name Novosphingobium marinum sp. nov. is proposed. The type strain is LA53(T) (?= CGMCC 1.12918(T)?= JCM 30307(T)). PMID:25424486

  8. Jatrophihabitans soli sp. nov., isolated from soil.

    PubMed

    Kim, Soo-Jin; Moon, Ji-Young; Lim, Jun-Muk; Hamada, Moriyuki; Ahn, Jae-Hyung; Weon, Hang-Yeon; Suzuki, Ken-Ichiro; Kwon, Soon-Wo

    2015-06-01

    One bacterial strain, designated KIS75-12T, isolated from a soil sample collected from Wonsando island located in Boryeong city, Republic of Korea, was characterized as aerobic, Gram-stain-positive, non-flagellated and a short rod. It grew between temperatures of 15-37 °C, pH 4-9 and 0-3.0 % (w/v) NaCl. The 16S rRNA gene analysis showed the strain was moderately related to Jatrophihabitans endophyticus S9-650T (97.7 %) and revealed low sequence similarity (?94.7 %) with all the other species with validly published names. Its major fatty acid was iso-C16 : 0. The predominant menaquinone of strain KIS75-12T was MK-9(H4). The polar lipids consisted of diphosphatidylglycerol and several small amounts of phosphatidylinositol, aminolipids and glycolipid. The peptidoglycan contained meso-A2pm as diagnostic diamino acid and the peptidoglycan type is A4?. The genomic DNA G+C content of the type strain was 72.1 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain KIS75-12T could be clearly distinguished from the only member of the genus Jatrophihabitans,J. endophyticus. Therefore, the results of this study indicate the existence of a representative of a novel species of the genus Jatrophihabitans, for which we propose the name Jatrophihabitans soli sp. nov., with strain KIS75-12T (?= KACC 17298T = DSM 45908T ?= NBRC 109658T) as the type strain. An emended description of the genus Jatrophihabitans is also given. PMID:25744581

  9. Niabella ginsenosidivorans sp. nov., isolated from compost.

    PubMed

    Yi, Kwon-Jung; Im, Wan-Taek; Kim, Dong-Woon; Liu, Qing Mei; Kim, Soo-Ki

    2015-11-01

    A Gram-reaction negative, strictly aerobic, non-motile, orange colored, and rod-shaped bacterium (designated BS26(T)) isolated from compost, was characterized by a polyphasic approach to clarify its taxonomic position. Strain BS26(T) was observed to grow optimally at 25-30°C and at pH 7.0 on R2A and nutrient media. Strain BS26(T) showed ß-glucosidase activity that was responsible for its ability to transform ginsenoside Rb1 (one of the active components of ginseng) to ginsenoside compound-K (C-K). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BS26(T) belongs to the genus Niabella of family Chitinophagaceae and was most closely related to Niabella soli DSM 19437(T) (94.5% similarity), N. yanshanensis CCBAU 05354(T) (94.3%), and N. aurantiaca DSM 17617(T) (93.8%). The G+C content of genomic DNA was 47.3 mol%. Chemotaxonomic data [predominant isoprenoid quinone-MK-7, major fatty acids-iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH, and summed feature 3 (comprising C16:1 ?7c and/or C16:1 ?6c)] supported the affiliation of strain BS26(T) to the genus Niabella. However, strain BS26(T) could be differentiated genotypically and phenotypically from the recognized species of the genus Niabella. The novel isolate therefore represents a novel species, for which the name Niabella ginsenosidivorans sp. nov. is proposed, with the type strain BS26(T) (=KACC 16620(T) =JCM 18199(T)). PMID:26502960

  10. Muriicola marianensis sp. nov., isolated from seawater.

    PubMed

    Hu, Jing; Zhang, Wei-Yan; Zhang, Xin-Qi; Hong-Cheng; Zhu, Xu-Fen; Wu, Min

    2015-02-01

    A Gram-stain-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated strain A6B8(T), was isolated from seawater of the Mariana Trench. The isolate grew at 4-50 °C (optimum 30-35 °C), at pH 6.5-8.0 (optimum pH 7.5) and with 0.5-4.0 % (w/v) NaCl (optimum 1.0-2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8(T) was related most closely to the genus Muriicola and shared highest sequence similarity of 97.7 % with Muriicola jejuensis EM44(T). Chemotaxonomic analysis showed menaquinone 6 (MK-6) was the predominant isoprenoid and iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH were the major cellular fatty acids. The polar lipid profile of strain A6B8(T) included phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA-DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8(T) and M. jejuensis EM44(T) were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, A6B8(T) (?= CGMCC 1.12606(T)?= KCTC 32436(T)) is considered to be the type strain of a novel species of the genus Muriicola, for which the name Muriicola marianensis sp. nov. is proposed. PMID:25376851

  11. Chryseobacterium shandongense sp. nov., isolated from soil.

    PubMed

    Yang, Fan; Liu, Hong-ming; Zhang, Rong; Chen, Ding-bin; Wang, Xiang; Li, Shun-peng; Hong, Qing

    2015-06-01

    YF-3T is a Gram-stain-negative, non-motile, non-spore-forming, yellow-orange, rod-shaped bacterium. Optimal growth conditions were at 30 °C, pH 7.0 and 1 % (w/v) NaCl. Phylogenetic analysis, on the basis of the 16S rRNA gene sequence, showed that strain YF-3T was closely related to the strains Chryseobacterium hispalense AG13T and Chryseobacterium taiwanense Soil-3-27T with 98.71 % and 96.93 % sequence similarity, respectively. Strain YF-3T contained MK-6 as the main menaquinone and had a polyamine pattern with sym-homospermidine as the major component. Its major polar lipid was phosphatidylethanolamine. The dominant fatty acids of strain YF-3T were iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 9 (comprising iso-C17 : 1?9c and/or C16 : 0 10-methyl) and summed feature 3 (comprising C16 : 1?7c and/or C16 : 1?6c). The DNA G+C content of strain YF-3T was 37 mol%. The DNA-DNA relatedness levels between strain YF-3T and the most closely related strains, C. hispalense AG13T and C. taiwanense Soil-3-27T, were 31.7 ± 2.1 % and 28.4 ± 5.4 %, respectively. Based on these results, a novel species named Chryseobacterium shandongense sp. nov. is proposed. The type strain is YF-3T (?= CCTCC AB 2014060T = JCM 30154T). PMID:25762725

  12. Flavobacterium buctense sp. nov., isolated from freshwater.

    PubMed

    Feng, Xiao-Min; Tan, Xu; Jia, Li; Long, Ping-Ping; Han, Lu; Lv, Jie

    2015-11-01

    A gram-negative, non-gliding motile, aerobic bacterium, designated as strain T7(T), was isolated from freshwater of Chishui River flowing through Maotai town, Guizhou Province, southwest of China. Based on the 16S rRNA gene sequence analysis, the isolate was identified as a member of the genus Flavobacterium and that shared less than 97 % sequence similarities with recognized Flavobacterium species. Its closest phylogenetic relative was Flavobacterium dankookense (96.9 %), followed by Flavobacterium cheonhonense (96.8 %) and Flavobacterium macrobrachii (96.7 %). The strain formed smooth yellow colonies on R2A plates, and cells were observed to be short rods. Strain T7(T) was found to be able to grow at 15-30 °C (optimum 25 °C), at NaCl concentration of 0-0.5 % (optimum 0 %) and at pH 6.5-9.5 (optimum pH 7.5). Catalase and oxidase tests were positive. Polar lipids of strain T7(T) included phosphatidylethanolamine, four unidentified polar lipids, one unidentified phospholipid and one unidentified aminolipid. Chemotaxonomic analysis revealed menaquinone-6 as the dominant respiratory quinone and C15:0, iso-C15:0 and iso-C15:1 as the major fatty acids. The DNA G+C content of strain T7(T) was determined to be 38.2 mol%. On the basis of phylogenetic, phenotypic and genetic data obtained in this study, strain T7(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium buctense sp. nov. is proposed. The type strain is T7(T) (=JCM 30750=CGMCC 1.15216). PMID:26374244

  13. Solimonas terrae sp. nov., isolated from soil.

    PubMed

    Kim, Soo-Jin; Moon, Ji-Young; Weon, Hang-Yeon; Ahn, Jae-Hyung; Chen, Wen-Ming; Kwon, Soon-Wo

    2014-04-01

    A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated strain KIS83-12(T), was isolated from soil of Gaui island in the Taean region of South Korea. The strain grew at 15-33 °C (optimum, 28 °C), at pH 5.0-8.0 (optimum, pH 7.0). Growth did not occur in the presence of NaCl. The strain was catalase-negative and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that KIS83-12(T) was most closely related to Solimonas soli DCY12(T) (96.9?%), Solimonas variicoloris MN28(T) (96.5?%), Solimonas flava CW-KD 4(T) (96.5?%) and Solimonas aquatica NAA16(T) (96.0?%), and formed a robust phyletic lineage with members of the genus Solimonas. The main isoprenoid quinone was Q-8. Major polar lipids included phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acids present in large and moderate amounts (>5.0?%) were summed feature 8 (C18?:?1?7c and/or C18?:?1?6c), C16?:?0, C16?:?1?5c, summed feature 2 (iso-C16?:?1 I and/or C14?:?0 3-OH) and C12?:?0. The DNA G+C content was 67.9 mol%. On the basis of the taxonomic data obtained in this study, KIS83-12(T) represents a novel species of the genus Solimonas, for which the name Solimonas terrae sp. nov. is proposed, with KIS83-12(T) (?=?KACC 16967(T)?=?DSM 27281(T)) as the type strain. PMID:24421276

  14. Degradation of car engine base oil by Rhodococcus sp. NDKK48 and Gordonia sp. NDKY76A.

    PubMed

    Koma, Daisuke; Sakashita, Yuichi; Kubota, Kenzo; Fujii, Yoshihide; Hasumi, Fumihiko; Chung, Seon-Yong; Kubo, Motoki

    2003-07-01

    Two microorganisms (NDKK48 and NDKY76A) that degrade long-chain cyclic alkanes (c-alkanes) were isolated from soil samples. Strains NDKK48 and NDKY76A were identified as Rhodococcus sp. and Gordonia sp., respectively. Both strains used not only normal alkane (n-alkane) but also c-alkane as a sole carbon and energy source, and the strains degraded more than 27% of car engine base oil (1% addition). PMID:12913308

  15. Effects of cell growth and a mobile genetic element on propagation of the phages SP16 and SP-beta in Bacillus subtilis

    E-print Network

    England, Eleina M. (Eleina Helen Maria)

    2014-01-01

    Two studies were performed on Bacillus subtilis phages SP16 and SP-beta to characterize the effects of growth and an integrative conjugative element, ICEBs1, respectively. I found that B. subtilis strains are significantly ...

  16. Skryjelites auritus gen. et sp. nov. and Quasimolites quasimodo gen. et sp. nov.-two new middle Cambrian hyolithids (?Mollusca) from the Czech Republic.

    PubMed

    Valent, Martin; Fatka, Old?ich; Szabad, Michal; Micka, Václav; Marek, Ladislav

    2015-01-01

    Two new endemic genera and species of extinct group of Hyolitha, Skryjelites auritus gen. et sp. nov. and Quasimolites quasimodo gen. et sp. nov. are described and illustrated from the Buchava Formation of the Barrandian area (Czech Republic). PMID:26623820

  17. In Vitro Antimicrobial Potential of the Lichen Parmotrema sp. Extracts against Various Pathogens

    PubMed Central

    Chauhan, Ritika; Abraham, Jayanthi

    2013-01-01

    Objective(s): The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. The perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. Lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. This study was aimed to explore in vitro antimicrobial activity of lichen Parmotrema sp. Material and Methods: The methanol and aqueous extracts of lichen Parmotrema sp. was extracted using Soxhlet extractor. Antibiotic assessment of methanol and aqueous extracts was done against eight bacterial (Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Shigella sp., Enterococci faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae,) clinical pathogens and five plant pathogenic fungal strains (Aspergillus terreus strain JAS1, Scedosporium sp. JAS1, Ganoderma sp. JAS4, Candida tropicalis and Fusarium sp.) by Kirby-Bauer method. Results: The methanol lichen Parmotrema sp. extract inhibited all the test organisms. The highest antibacterial activity was found against Pseudomonas aeruginosa and Staphylococcus aureus. The weakest activity was manifested in Salmonella sp. and Scedosporium sp. JAS1. Strong antifungal effect was found against Ganoderma sp. JAS4 and Fusarium sp. The aqueous lichen Parmotrema sp. extract revealed neither antibacterial nor antifungal activity. Conclusion: The present study shows that tested lichen Parmotrema sp. extracts demonstrated a strong antimicrobial effect. That suggests the active components from methanol extracts of the investigated lichen Parmotrema sp. can be used as natural antimicrobial agent against pathogens. PMID:23997920

  18. Gas Generation Equations for CRiSP 1.6 April 21, 1998 1 Gas Generation Equations for CRiSP 1.6

    E-print Network

    Washington at Seattle, University of

    Gas Generation Equations for CRiSP 1.6 April 21, 1998 1 Gas Generation Equations for CRiSP 1.6 Theory For CRiSP.1.6 new equations have been implemented for gas production from spill. As a part, and a bounded exponential function of the spill on a per spillbay basis. Work was then done, using CRiSP, to fit

  19. On the fourth Diadema species (Diadema-sp) from Japan.

    PubMed

    Chow, Seinen; Kajigaya, Yoshikazu; Kurogi, Hiroaki; Niwa, Kentaro; Shibuno, Takuro; Nanami, Atsushi; Kiyomoto, Setuo

    2014-01-01

    Four long-spined sea urchin species in the genus Diadema are known to occur around the Japanese Archipelago. Three species (D. savignyi, D. setosum, and D. paucispinum) are widely distributed in the Indo-Pacific Ocean. The fourth species was detected by DNA analysis among samples originally collected as D. savignyi or D. setosum in Japan and the Marshall Islands and tentatively designated as Diadema-sp, remaining an undescribed species. We analyzed nucleotide sequences of the cytochrome oxidase I (COI) gene in the "D. savignyi-like" samples, and found all 17 individuals collected in the mainland of Japan (Sagami Bay and Kyushu) to be Diadema-sp, but all nine in the Ryukyu Archipelago (Okinawa and Ishigaki Islands) to be D. savignyi, with large nucleotide sequence difference between them (11.0%±1.7 SE). Diadema-sp and D. savignyi shared Y-shaped blue lines of iridophores along the interambulacrals, but individuals of Diadema-sp typically exhibited a conspicuous white streak at the fork of the Y-shaped blue iridophore lines, while this feature was absent in D. savignyi. Also, the central axis of the Y-shaped blue lines of iridophores was approximately twice as long as the V-component in D. savignyi whereas it was of similar length in Diadema-sp. Two parallel lines were observed to constitute the central axis of the Y-shaped blue lines in both species, but these were considerably narrower in Diadema-sp. Despite marked morphological and genetic differences, it appears that Diadema-sp has been mis-identified as D. savignyi for more than half a century. PMID:25054386

  20. On the Fourth Diadema Species (Diadema-sp) from Japan

    PubMed Central

    Chow, Seinen; Kajigaya, Yoshikazu; Kurogi, Hiroaki; Niwa, Kentaro; Shibuno, Takuro; Nanami, Atsushi; Kiyomoto, Setuo

    2014-01-01

    Four long-spined sea urchin species in the genus Diadema are known to occur around the Japanese Archipelago. Three species (D. savignyi, D. setosum, and D. paucispinum) are widely distributed in the Indo-Pacific Ocean. The fourth species was detected by DNA analysis among samples originally collected as D. savignyi or D. setosum in Japan and the Marshall Islands and tentatively designated as Diadema -sp, remaining an undescribed species. We analyzed nucleotide sequences of the cytochrome oxidase I (COI) gene in the “D. savignyi-like” samples, and found all 17 individuals collected in the mainland of Japan (Sagami Bay and Kyushu) to be Diadema-sp, but all nine in the Ryukyu Archipelago (Okinawa and Ishigaki Islands) to be D. savignyi, with large nucleotide sequence difference between them (11.0%±1.7 SE). Diadema-sp and D. savignyi shared Y-shaped blue lines of iridophores along the interambulacrals, but individuals of Diadema-sp typically exhibited a conspicuous white streak at the fork of the Y-shaped blue iridophore lines, while this feature was absent in D. savignyi. Also, the central axis of the Y-shaped blue lines of iridophores was approximately twice as long as the V-component in D. savignyi whereas it was of similar length in Diadema-sp. Two parallel lines were observed to constitute the central axis of the Y-shaped blue lines in both species, but these were considerably narrower in Diadema-sp. Despite marked morphological and genetic differences, it appears that Diadema-sp has been mis-identified as D. savignyi for more than half a century. PMID:25054386

  1. Sp1 regulates the transcription of BMPR1A

    PubMed Central

    Dahdaleh, Fadi S.; Carr, Jennifer C.; Calva, Daniel; Howe, James R.; Howe, James R.

    2011-01-01

    Background BMPR1A is a cell surface receptor in the Bone Morphogenetic Protein (BMP) pathway. Mutations in BMPR1A predispose to Juvenile Polyposis (JP). Sp1 and related proteins are widely expressed regulators of gene transcription, including members of the BMP pathway. We set out to identify important transcription factor binding sites (TFBS) in the recently identified BMPR1A promoter and to assess for the role of Sp1 and associated proteins in its regulation. Materials and Methods The BMPR1A promoter was cloned into a Luciferase reporter vector. Deletion fragments of this promoter insert were then constructed, of varying lengths and opposing directions and were used to transfect HEK-293 and CRL-1459 cells. In silico analysis was performed to screen for relevant TFBS. Site-Directed Mutagenesis (SDM) was then employed to individually disrupt these TFBS in the wild-type (WT) vector. SDM constructs were then assessed for activity. Results Light activity from the deletion constructs ranged between 3 and 129% of the WT promoter. ModelInspector identified 8 potential binding sites for Sp1 and Sp1-associated proteins that mapped to areas of marked loss or gain of activity from the deletion constructs. SDM of these TFBS led to a drop in activity in five mutants, which included 3 Sp1 sites, an ETSF site and NFKB site. Conclusions By combining in silico analysis and experimental data, Sp1 was found to be a candidate factor that likely plays a role in the transcriptional regulation of BMPR1A. This study potentially provides further insight toward the molecular basis of JP, and suggests that Sp1 plays a role in BMP signaling. PMID:21872883

  2. 290I-SP13-Syllabus.doc; rev 4, 2/3/13 Sustainable Manufacturing, E290I, SP 2013

    E-print Network

    Frenklach, Michael

    290I-SP13-Syllabus.doc; rev 4, 2/3/13 Sustainable Manufacturing, E290I, SP 2013 Tu/Th 9:30AM-11 and specializes in assessing social sustainability and sustainable manufacturing. #12;290I-SP13-Syllabus.doc; rev

  3. Draft Genome Sequence of Methylobacterium sp. ME121, Isolated from Soil as a Mixed Single Colony with Kaistia sp. 32K

    PubMed Central

    Fujinami, Shun; Takeda-Yano, Kiyoko; Onodera, Takefumi; Satoh, Katsuya; Shimizu, Tetsu; Wakabayashi, Yuu; Narumi, Issay; Nakamura, Akira

    2015-01-01

    Methylobacterium sp. ME121 was isolated from soil as a mixed single colony with Kaistia sp. 32K, and its growth was enhanced by coculture. Here, we report the draft genome sequence of Methylobacterium sp. ME121, which may contribute to the study of the molecular mechanisms underlying this phenomenon. PMID:26337893

  4. 75 FR 42380 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Contract and SP-15 Financial Day-Ahead...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ...COMMISSION Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Contract and SP-15 Financial Day-Ahead LMP Off-Peak Contract Offered for...determination whether the SP-15 \\2\\ Financial Day-Ahead LMP Peak (``SPM'') contract...

  5. Lutibacter crassostreae sp. nov., isolated from oyster.

    PubMed

    Park, Sooyeon; Park, Ji-Min; Won, Sung-Min; Park, Doo-Sang; Yoon, Jung-Hoon

    2015-08-01

    A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and ovoid or rod-shaped bacterial strain, designated TYO-8T, was isolated from an oyster collected from the South Sea in South Korea. Strain TYO-8T grew optimally at 25?°C, at pH?7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain TYO-8T fell within the clade comprising the type strains of species of the genus Lutibacter, clustering coherently with the type strain of Lutibacter litoralis with a sequence similarity of 99.3%. Strain TYO-8T exhibited 16S rRNA gene sequence similarity values of 95.3-97.5% to the type strains of other species of the genus Lutibacter and of less than 92.9% to the type strains of other species with validly published names. Strain TYO-8T contained MK-6 as the predominant menaquinone and iso-C15?:?0, iso-C15?:?0 3-OH and iso-C15?:?1 G as the major fatty acids. The major polar lipids of strain TYO-8T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain TYO-8T was 33.8?mol% and its DNA-DNA relatedness values with the type strains of L. litoralis, Lutibacter aestuarii and Lutibacter flavus were 13-27%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain TYO-8T is distinct from other species of the genus Lutibacter. On the basis of the data presented, strain TYO-8T is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter crassostreae sp. nov. is proposed. The type strain is TYO-8T (?=?KCTC 42461T?=?NBRC 110923T). PMID:25969474

  6. Enantiospecific sp2-sp3 coupling of secondary and tertiary boronic esters

    NASA Astrophysics Data System (ADS)

    Bonet, Amadeu; Odachowski, Marcin; Leonori, Daniele; Essafi, Stephanie; Aggarwal, Varinder K.

    2014-07-01

    The cross-coupling of boronic acids and related derivatives with sp2 electrophiles (the Suzuki-Miyaura reaction) is one of the most powerful C-C bond formation reactions in synthesis, with applications that span pharmaceuticals, agrochemicals and high-tech materials. Despite the breadth of its utility, the scope of this Nobel prize-winning reaction is rather limited when applied to aliphatic boronic esters. Primary organoboron reagents work well, but secondary and tertiary boronic esters do not (apart from a few specific and isolated examples). Through an alternative strategy, which does not involve using transition metals, we have discovered that enantioenriched secondary and tertiary boronic esters can be coupled to electron-rich aromatics with essentially complete enantiospecificity. As the enantioenriched boronic esters are easily accessible, this reaction should find considerable application, particularly in the pharmaceutical industry where there is growing awareness of the importance of, and greater clinical success in, creating biomolecules with three-dimensional architectures.

  7. Methanosalsum natronophilum sp. nov., and Methanocalculus alkaliphilus sp. nov., haloalkaliphilic methanogens from hypersaline soda lakes.

    PubMed

    Sorokin, Dimitry Y; Abbas, Ben; Merkel, Alexander Y; Rijpstra, W Irene C; Damsté, Jaap S Sinninghe; Sukhacheva, Marina V; van Loosdrecht, Mark C M

    2015-10-01

    Two groups of haloalkaliphilic methanogenic archaea were dominating in enrichments from hypersaline soda lake sediments at pH?10. At moderate salt concentrations with formate or H2 as electron donor, methanogens belonging to the genus Methanocalculus were enriched, while at high salt concentrations with methylated substrates, a group related to Methanosalsum zhilinae was dominating. For both groups, several pure cultures were obtained including the type strains AMF2T for the Methanocalculus group and AME2T for the Methanosalsum group. The Methanocalculus group is characterized by lithoheterotrophic growth with either formate (preferable substrate) or H2 at moderate salinity up to 1.5-2?M total Na+ and obligate alkaliphilic growth with an optimum at pH?9.5. According to phylogenetic analysis, the group also includes closely related strains isolated previously from the low-salt alkaline Lonar Lake. The novel Methanosalsum group is characterized by high salt tolerance (up to 3.5?M total Na+) and obligate alkaliphilic growth with an optimum at pH?9.5. It has a typical methylotrophic substrate profile, utilizing methanol, methylamines and dimethyl sulfide (at low concentrations) as methanogenic substrates. On the basis of physiological and phylogenetic data, it is proposed that the two groups of soda lake methanogenic isolates are assigned into two novel species, Methanocalculus alkaliphilus sp. nov. (type strain AMF2T?=?DSM 24457T?=?UNIQEM U859T) and Methanosalsum natronophilum sp. nov. (type strain AME2T?=?DSM 24634T?=?NBRC 110091T). PMID:26228570

  8. Humibacter ginsengiterrae sp. nov., and Humibacter ginsengisoli sp. nov., isolated from soil of a ginseng field.

    PubMed

    Kim, Eul-Kon; Hoang, Van-An; Kim, Yeon-Ju; Nguyen, Ngoc-Lan; Sukweenadhi, Johan; Kang, Jong-Pyo; Yang, Deok-Chun

    2015-08-01

    Two novel Gram-staining-positive bacteria, designated DCY60T and DCY90T, were isolated from soil of a ginseng field in the Republic of Korea. 16S rRNA gene sequence comparisons showed the two novel strains were closely related to members of the genus Humibacter with greatest similarity to Humibacter antri KCTC 33009T (98.8 and 98.4% for DCY60T and DCY90T, respectively). The predominant menaquinones present were MK-11 and MK-12. The major fatty acids were anteiso-C17?:?0 and summed feature 8 containing C18?:?1?7c and/or C18?:?1?6c. The DNA G+C contents of strains DCY60T and DCY90T were 62.8 and 66.8?mol%, respectively. The peptidoglycan of both strains contained the amino acids ornithine, 2,4-diaminobutyric acid, alanine, glutamic acid and glycine. The cell-wall sugars of strain DCY60T comprised glucose, galactose, rhamnose and xylose, while strain DCY90T contained glucose, galactose, rhamnose and ribose. The major polar lipids of both strains were phosphatidylglycerol, an unidentified glycolipid, and an unknown phospholipid. On the basis of the phenotypic analysis strains DCY60T and DCY90T represent novel species of the genus Humibacter, for which names Humibacter ginsengiterrae sp. nov. (type strain DCY60T?=?KCTC 33520T?=?JCM 30079T) and Humibacter ginsengisoli sp. nov. (type strain DCY90T?=?KCTC 33521T?=?JCM 30080T) are proposed. PMID:25977283

  9. Nocardioides szechwanensis sp. nov. and Nocardioides psychrotolerans sp. nov., isolated from a glacier.

    PubMed

    Liu, Qing; Xin, Yu-hua; Liu, Hong-can; Zhou, Yu-guang; Wen, Ying

    2013-01-01

    Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT(1)-17(T) and RHLT(2)-1(T)) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Nocardioides and shared 97.8?% sequence similarity with each other and 97.6 and 98.4 % sequence similarity, respectively, with Nocardioides kribbensis KSL-2(T). Strain RHLT(1)-17(T) grew at 0-35 °C and strain RHLT(2)-1(T) grew at 0-25 °C. The major cellular fatty acids of strain RHLT(1)-17(T) were C(17 : 1)?8c (32.69 %) and iso-C(16 : 0) (21.74 %). The major cellular fatty acids of strain RHLT(2)-1(T) were C(18 : 1)?9c (28.72 %), summed feature 3 (17.14 %; comprising C(16 : 1)?7c and/or C(16 : 1)?6c), iso-C(16 : 0) (14.35 %), C(16 : 0) (9.96 %) and iso-C(14 : 0) (8.34 %). Both strains contained ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H(4)) as the predominant menaquinone. On the basis of data obtained using a polyphasic approach, two novel species, Nocardioides szechwanensis sp. nov. (type strain RHLT(1)-17(T) = CGMCC 1.11147(T) = NBRC 108562(T)) and Nocardioides psychrotolerans sp. nov. (type strain RHLT(2)-1(T) =CGMCC 1.11156(T) = NBRC 108563(T)), are proposed. PMID:22345140

  10. Glycomyces fuscus sp. nov. and Glycomyces albus sp. nov., actinomycetes isolated from a hypersaline habitat.

    PubMed

    Han, Xiao-Xue; Luo, Xiao-Xia; Zhang, Li-Li

    2014-07-01

    Two actinomycete strains, designated TRM 49117(T) and TRM 49136(T), were isolated from a hypersaline habitat in Xinjiang Province, north-west China and were characterized taxonomically by using a polyphasic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain TRM 49117(T) had 93.93% similarity with the type strain Glycomyces halotolerans TRM 40137(T) (GenBank accession no. HQ651156) and TRM 49136(T) had 94.32% similarity with G. halotolerans TRM 40137(T). The 16S rRNA gene sequence similarity between the two new isolates was 93%. The isolates contained meso-diaminopimelic acid as the diagnostic diamino acid and anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 as major cellular fatty acids. The predominant menaquinones of the isolates were MK-9(H4) and MK-9(H6). The whole-cell sugar patterns of these strains contained xylose and ribose, and strain TRM 49136(T) also contained arabinose. The polar lipid pattern of strain TRM 49117(T) comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylcholine, phosphatidylinositol and three additional unknown phospholipids. The polar lipid pattern of strain TRM 49136(T) comprised phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, glycolipids and two phosphoglycolipids of unknown composition. Genotypic and phenotypic data confirmed that strains TRM 49117(T) and TRM 49136(T) represent two novel species, clearly different from related species of the genus Glycomyces, for which the names Glycomyces fuscus sp. nov. (type strain TRM 49117(T)?= CCTCC AA 2013003(T)?= NRRL B-59998(T)?= KACC 17682(T)) and Glycomyces albus sp. nov. (type strain TRM 49136(T)?= CCTCC AA 2013004(T)?= NRRL B-24927(T)?= KACC 17681(T)) are proposed. PMID:24776532

  11. Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov., isolated from soil.

    PubMed

    Zhang, Lei; Bai, Juan; Wang, Yang; Wu, Gao-Lin; Dai, Jun; Fang, Cheng-Xiang

    2011-09-01

    Two Gram-reaction-negative, rod-shaped, gliding, yellow-pigmented bacterial strains, designated ZLD-17(T) and ZLD-29(T), were isolated from arid soil samples collected from Xinjiang Province, north-west China, and subjected to analysis using a polyphasic taxonomic approach. Both novel strains required 1.0-2.0?% (w/v) sea salts for optimal growth. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genus Lysobacter within the class Gammaproteobacteria. Strain ZLD-17(T) showed highest 16S rRNA gene sequence similarities to Lysobacter capsici KCTC 22007(T) (96.9?%), Lysobacter spongiicola DSM 21749(T) (96.8?%) and Lysobacter koreensis KCTC 12204(T) (96.8?%), whereas strain ZLD-29(T) showed highest sequence similarities to Lysobacter niastensis DSM 18481(T) (96.0?%) and Lysobacter enzymogenes DSM 2043(T) (95.9?%). 16S rRNA gene sequence similarity between ZLD-17(T) and ZLD-29(T) was 96.1?%. The DNA G+C contents of strains ZLD-17(T) and ZLD-29(T) were 67.9 and 68.2 mol%, respectively. The major cellular fatty acids of both strains were summed feature 3 (iso-C??:? 2-OH and/or C??:??7c), iso-C??:??9c, iso-C??:?, C??:? and iso-C??:? 3-OH; their predominant isoprenoid quinone was Q-8 and their major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on their phenotypic characteristics, phylogenetic position as determined by 16S rRNA gene sequence analysis and chemotaxonomic data, strains ZLD-17(T) (?=?CCTCC AB 207174(T) ?=?KCTC 23076(T)) and ZLD-29(T) (?=?CCTCC AB 207175(T)?=?KCTC 23077(T)) represent two novel species of the genus Lysobacter, for which the names Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov. are proposed, respectively. PMID:20952549

  12. Bradyrhizobium pachyrhizi sp. nov. and Bradyrhizobium jicamae sp. nov., isolated from effective nodules of Pachyrhizus erosus.

    PubMed

    Ramírez-Bahena, Martha Helena; Peix, Alvaro; Rivas, Raúl; Camacho, María; Rodríguez-Navarro, Dulce N; Mateos, Pedro F; Martínez-Molina, Eustoquio; Willems, Anne; Velázquez, Encarna

    2009-08-01

    Several strains isolated from the legume Pachyrhizus erosus were characterized on the basis of diverse genetic, phenotypic and symbiotic approaches. These novel strains formed two groups closely related to Bradyrhizobium elkanii according to their 16S rRNA gene sequences. Strains PAC48T and PAC68T, designated as the type strains of these two groups, presented 99.8 and 99.1% similarity, respectively, in their 16S rRNA gene sequences with respect to B. elkanii USDA 76T. In spite of these high similarity values, the analysis of additional phylogenetic markers such as atpD and glnII genes and the 16S-23S intergenic spacer (ITS) showed that strains PAC48T and PAC68T represented two separate novel species of the genus Bradyrhizobium with B. elkanii as their closest relative. Phenotypic differences among the novel strains isolated from Pachyrhizus and B. elkanii were found regarding the assimilation of carbon sources and antibiotic resistance. All these differences were congruent with DNA-DNA hybridization analysis which revealed 21% genetic relatedness between strains PAC48T and PAC68T and 46% and 25%, respectively, between these strains and B. elkanii LMG 6134T. The nodD and nifH genes of strains PAC48T and PAC68T were phylogenetically divergent from those of bradyrhizobia species that nodulate soybean. Soybean was not nodulated by the novel Pachyrhizus isolates. Based on the genotypic and phenotypic data obtained in this study, the new strains represent two novel species for which the names Bradyrhizobium pachyrhizi sp. nov. (type strain PAC48T=LMG 24246T=CECT 7396T) and Bradyrhizobium jicamae sp. nov. (type strain PAC68T=LMG 24556T=CECT 7395T) are proposed. PMID:19567584

  13. Hymenobacter koreensis sp. nov. and Hymenobacter saemangeumensis sp. nov., isolated from estuarine water.

    PubMed

    Kang, Ji Young; Chun, Jeesun; Choi, Ahyoung; Moon, Sung Hyun; Cho, Jang-Cheon; Jahng, Kwang Yeop

    2013-12-01

    Two Gram-reaction-negative, rod-shaped, non-motile and red-pink-pigmented bacterial strains, designated GYR3077(T) and GSR0100(T), were isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do, South Korea, and were characterized using a polyphasic approach. 16S rRNA genes of strains GYR3077(T) and GSR0100(T) exhibited sequence similarities of 95.9?% to Hymenobacter deserti ZLB-3(T) and 96.6?% to Hymenobacter soli PB17(T), respectively, and indicated that these isolates belonged to the phylum Bacteroidetes. The major cellular fatty acids present in the two isolates were iso-C15?:?0, C16?:?1?5c, summed feature 4 (iso-C17?:?1 I and/or anteiso-C17?:?1 B) and summed feature 3 (C16?:?1?7c and/or C16?:?1?6c). The major respiratory quinone and polyamine patterns were menaquinone-7 and sym-homospermidine, characteristic of the genus Hymenobacter. Flexirubin-type pigments were absent in both strains. The DNA G+C contents of strains GYR3077(T) and GSR0100(T) were 60.2 mol% and 61.9 mol%, respectively. The major polar lipid of strains GYR3077(T) and GSR0100(T) was phosphatidylethanolamine. Based on the morphological and physiological properties, strains GYR3077(T) and GSR0100(T) were considered to represent two novel species of the genus Hymenobacter, for which the names Hymenobacter koreensis sp. nov. (type strain GYR3077(T)?=?KACC 16451(T)?=?JCM 17924(T)) and Hymenobacter saemangeumensis sp. nov. (type strain GSR0100(T)?=?KACC 16452(T)?=?JCM 17923(T)) are proposed. PMID:23907229

  14. Pichia dushanensis sp. nov. and Hyphopichia paragotoi sp. nov., two sexual yeast species associated with insects and rotten wood.

    PubMed

    Ren, Yong-Cheng; Liu, Si-Tong; Li, Ying; Hui, Feng-Li

    2015-09-01

    Seven yeast strains were isolated from the gut of insect larvae and decayed wood, which were collected from three localities near Nanyang, Henan Province, China. These strains were identified as two novel species through comparison of sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and other taxonomic characteristics. Pichia dushanensis sp. nov. was closely related to species in the Pichia clade and produced one to four spheroid ascospores in a deliquescent ascus. The D1/D2 sequence of P. dushanensis sp. nov. differed from its closest relative, Issatchenkia (Pichia) sp. NRRL Y-12824, by 3.6% sequence divergence (16 substitutions and 4 gaps). The species also differed from its four closest known species, Candida rugopelliculosa, Pichia occidentalis, Pichia exigua and Candida phayaonensis, by 4.1-4.4% sequence divergence (22-24 substitutions and 0-2 gaps) in the D1/D2 sequences. Hyphopichia paragotoi sp. nov. belonged to the Hyphopichia clade, and its nearest phylogenetic neighbours were Candida gotoi, Candida pseudorhagii, Candida rhagii and Hyphopichia heimii with 3.2-4.2% sequence divergence (16-21 substitutions and 1 gap) in the D1/D2 sequences. In comparison with previously established species, H. paragotoi sp. nov. formed one hat-shaped ascospore in a persistent ascus. The type strain of P. dushanensis sp. nov. is NYNU 14658(T) (?= CICC 33049(T) = CBS 13912(T)), and the type strain of H. paragotoi sp. nov. is NYNU 14666(T) (?= CICC 33048(T) = CBS 13913(T)). PMID:25999593

  15. Bioremediation of crude oil contamination with Acinetobacter sp. A3.

    PubMed

    Hanson, K G; Nigam, A; Kapadia, M; Desai, A J

    1997-09-01

    Acinetobacter sp. A3 is able to extensively degrade Bombay High Crude Oil (BHCO) and utilize it as the sole source of carbon. A total degradation of 70% BHCO was noted by the end of 120 h of growth of Acinetobacter sp. A3 under shake flask condition, 60% of which was due to biodegradation. In crude oil-contaminated soil (5%) amended with Acinetobacter sp. A3, there was both an increase in colony-forming units (CFU) and crude oil degradation. This is in contrast to a decrease in CFU of the indigenous microorganisms and lower degradation in unamended soil within the same 30-day period. Also, Acinetobacter sp. A3-treated soil permitted better germination of Mung beans (Phaseolus aureus) and growth as evidenced by better length and weight of the plants and chlorophyll content of its leaves, which was attributed to the reduction in phytotoxicity of the crude oil owing to its degradation. This crude oil degradative capability of Acinetobacter sp. A3 could be exploited for bioremediation purposes. PMID:9236304

  16. Oil productivity of the tropical marine diatom Thalassiosira sp.

    PubMed

    Nurachman, Zeily; Hartati; Anita, Syahfitri; Anward, Etsuroyya Ewidyasari; Novirani, Gestria; Mangindaan, Bill; Gandasasmita, Suryo; Syah, Yana Maolana; Panggabean, Lily Maria Goretty; Suantika, Gede

    2012-03-01

    To understand the potential of cultivating tropical marine diatom Thalassiosira sp. to produce biofuel, biodiesel product properties and growth characteristics of Thalassiosira sp. in three different media were investigated. After medium evaluation, significant Thalassiosira sp. cell growth was observed in both Walne and enriched seawater media, but not in plain seawater medium. The microalgae grew well in alkaline condition (pH range of 8.0-8.8). The average biomass density cultured in Walne and enriched seawater media on the 6th day was 4.36 and 2.50 g L(-1), respectively. Based on ESI-IT-MS spectra, the TAGs of algal oil were identified as POP, POO, and SOO, and the FAMEs as oleic acid methyl ester. The oil productivity of Thalassiosira sp. cultured in Walne and enriched seawater media were 150 and 290 ?L L(-1) d(-1), respectively. The density and kinematic viscosity of Thalassiosira sp. biodiesel were 0.857 g mL(-1) and 1.151 mm(2) s(-1). PMID:22264432

  17. Evolution of space food in Nostoc sp. HK-01

    NASA Astrophysics Data System (ADS)

    Tomita-Yokotani, Kaori; Yamashita, Masamichi; Hashimoto, Hirofumi; Sato, Seigo; Kimura, Yasuko; Katoh, Hiroshi; Arai, Mayumi

    2012-07-01

    Habitation in outer space is one of our challenges. We have been studying future space agriculture to provide food and oxygen for the habitation area in the space environment, on Mars. A cyanobacteria, Nostoc sp. HK-01, has high several outer space environmental tolerance. We have already confirmed that Nostoc sp.HK-01 had an ability to grow for over several years on the Martian regolith simulant in a laboratory experiment. Nostoc sp HK-01 would have high contribution to change the atmosphere in Mars as a photosynthetic creature. In outer environment, all of materials have to circulate for all of creature living in artificial eco-systems on Mars. This material has several functions as the utilization in space agriculture. Here, we are proposing using them as a food after its growing on Mars. We are trying to determine the best conditions and evolution for space food using Nostoc sp.HK-01 and studying the proposal of utilization of cyanobacteria, Nostoc sp HK-01, for the variation of meal as space agriculture.

  18. Effects of Oligomerization and Secondary Structure on the Surface Behavior of Pulmonary Surfactant Proteins SP-B and SP-C

    PubMed Central

    Wüstneck, N.; Wüstneck, R.; Perez-Gil, J.; Pison, U.

    2003-01-01

    The relationship among protein oligomerization, secondary structure at the interface, and the interfacial behavior was investigated for spread layers of native pulmonary surfactant associated proteins B and C. SP-B and SP-C were isolated either from butanol or chloroform/methanol lipid extracts that were obtained from sheep lung washings. The proteins were separated from other components by gel exclusion chromatography or by high performance liquid chromatography. SDS gel electrophoresis data indicate that the SP-B samples obtained using different solvents showed different oligomerization states of the protein. The CD and FTIR spectra of SP-B isolated from all extracts were consistent with a secondary structure dominated by ?-helix. The CD and FTIR spectra of the first SP-C corresponded to an ?-helical secondary structure and the spectra of the second SP-C corresponded to a mixture of ?-helical and ?-sheet conformation. In contrast, the spectra of the third SP-C corresponded to antiparallel ?-sheets. The interfacial behavior was characterized by surface pressure/area (?-A) isotherms. Differences in the oligomerization state of SP-B as well as in the secondary structure of SP-C all produce significant differences in the surface pressure/area isotherms. The molecular cross sections determined from the ?-A isotherms and from dynamic cycling experiments were 6 nm2/dimer molecule for SP-B and 1.15 nm2/molecule for SP-C in ?-helical conformation and 1.05 nm2/molecule for SP-C in ?-sheet conformation. Both the oligomer ratio of SP-B and the secondary structure of SP-C strongly influence organization and behavior of these proteins in monolayer assemblies. In addition, ?-helix ? ?-sheet conversion of SP-C occurs simply by an increase of the summary protein/lipid concentration in solution. PMID:12609896

  19. SP-100 program users handbook basic configurational tradeoffs

    SciTech Connect

    Manvi, R.; Fujita, T.

    1986-03-15

    This document addresses basic configurational tradeoffs associated with the SP-100 class of nuclear space power systems. In the SP-100 project, the three reference designs, fallback, baseline, and advanced, employ thermoelectric power conversion with different thermoelectric materials. This issue of the document presents results generated for the thermoelectric design as of the end of 1985. Emphasis is placed on showing general trends and the range of possibilities that could result from selection of a particular design. This document, which reflects efforts on 100-kWe reference designs as of the end of 1985, will be extended and updated to reflect progress in the design studies of the follow-on ground engineering phase for which a 300-kWe size has been selected. THere is a considerable flexibility to the SP-100 power system so that it will generally be most advantageous to tailor it to meet user requirements.

  20. Biochemical changes induced by fungicides in nitrogen fixing Nostoc sp.

    PubMed

    Deviram, G V N S; Pant, Gaurav; Prasuna, R Gyana

    2013-01-01

    The present study indicates the effect of fungicides (approved by WHO) and their behavior on nitrogen fixer of rice eco system Nostoc sp. Application of plant protecting chemicals at recommended levels braced up the growth of blue green algae thereby enhancing heterocyst formation and nitrogenase activity. Nostoc sp demoed varying degrees of sensitivity to fungicides. Biomass yield, protein, carbohydrate content reduced after 3pg/mL concentration. Heterocyst damage was observed from 4?g/mL, Proline content increased with increase in fungicide concentration, utmost yellowing of the culture started from 4?g/mL. The decreasing order of the toxicity to Nostoc sp with fungicides was Mancozeb> Ediphenphos> Carbendazim> Hexaconazole. PMID:25508341

  1. Biochemical changes induced by fungicides in nitrogen fixing Nostoc sp.

    PubMed

    Deviram, G V N S; Pant, Gaurav; Prasuna, R Gyana

    2013-01-01

    The present study indicates the effect of fungicides (approved by WHO) and their behavior on nitrogen fixer of rice eco system Nostoc sp. Application of plant protecting chemicals at recommended levels braced up the growth of blue green algae thereby enhancing heterocyst formation and nitrogenase activity. Nostoc sp demoed varying degrees of sensitivity to fungicides. Biomass yield, protein, carbohydrate content reduced after 3pg/mL concentration. Heterocyst damage was observed from 4?g/mL, Proline content increased with increase in fungicide concentration, utmost yellowing of the culture started from 4?g/mL. The decreasing order of the toxicity to Nostoc sp with fungicides was Mancozeb> Ediphenphos> Carbendazim> Hexaconazole. PMID:25464710

  2. Interrelationships between Bacillus sp. CHEP5 and Bradyrhizobium sp. SEMIA6144 in the induced systemic resistance against Sclerotium rolfsii and symbiosis on peanut plants.

    PubMed

    Figueredo, Maria Soledad; Tonelli, Maria Laura; Taurian, Tania; Angelini, Jorge; Ibanez, Fernando; Valetti, Lucio; Munoz, Vanina; Anzuay, Maria Soledad; Luduena, Liliana; Fabra, Adriana

    2014-12-01

    Plant-growth-promoting bacteria are often used to enhance crop yield and for biological control of phytopathogens. Bacillus sp. CHEP5 is a biocontrol agent that induces systemic resistance (ISR) in Arachis hypogaea L. (peanut) against Sclerotium rolfsii, the causal agent of root and stem wilt. In this work, the effect of the co-inoculation of Bacillus sp. CHEP5 and the peanut nodulating strain Bradyrhizobium sp. SEMIA 6144 was studied on induction of both systemic resistance and nodulation processes. Bradyrhizobium sp. SEMIA 6144 did not affect the ability of Bacillus sp. CHEP5 to protect peanut plants from S. rolfsii by ISR and the priming in challenged-plants, as evidenced by an increment in phenylalanine ammonia-lyase enzyme activity. Additionally, the capacity of Bradyrhizobium sp. SEMIA 6144 to induce nodule formation in pathogen-challenged plants was improved by the presence of Bacillus sp. CHEP5. PMID:25431416

  3. On the trajectories of Sp(1) -Kepler problems

    NASA Astrophysics Data System (ADS)

    Meng, Guowu

    2015-10-01

    The classical Sp(1) -Kepler problems are formulated with the help of an idea from S. Sternberg. The trajectories of these models are determined via an idea originated from Levi-Civita. It is found that, for a non-colliding trajectory, its shadow-its projection to the external configuration space-is an ellipse, a parabola or a branch of hyperbola according as the total energy is negative, zero or positive. Moreover, it is shown that, for the Sp(1) -Kepler problems at level n ? 2, the group SL(n, H) ×R+ acts transitively on both the set of elliptic shadow trajectories and the set of parabolic shadow trajectories.

  4. SpF: Enabling Petascale Performance for Pseudospectral Dynamo Models

    NASA Astrophysics Data System (ADS)

    Jiang, W.; Clune, T.; Vriesema, J.; Gutmann, G.

    2013-12-01

    Pseudospectral (PS) methods possess a number of characteristics (e.g., efficiency, accuracy, natural boundary conditions) that are extremely desirable for dynamo models. Unfortunately, dynamo models based upon PS methods face a number of daunting challenges, which include exposing additional parallelism, leveraging hardware accelerators, exploiting hybrid parallelism, and improving the scalability of global memory transposes. Although these issues are a concern for most models, solutions for PS methods tend to require far more pervasive changes to underlying data and control structures. Further, improvements in performance in one model are difficult to transfer to other models, resulting in significant duplication of effort across the research community. We have developed an extensible software framework for pseudospectral methods called SpF that is intended to enable extreme scalability and optimal performance. High-level abstractions provided by SpF unburden applications of the responsibility of managing domain decomposition and load balance while reducing the changes in code required to adapt to new computing architectures. The key design concept in SpF is that each phase of the numerical calculation is partitioned into disjoint numerical 'kernels' that can be performed entirely in-processor. The granularity of domain-decomposition provided by SpF is only constrained by the data-locality requirements of these kernels. SpF builds on top of optimized vendor libraries for common numerical operations such as transforms, matrix solvers, etc., but can also be configured to use open source alternatives for portability. SpF includes several alternative schemes for global data redistribution and is expected to serve as an ideal testbed for further research into optimal approaches for different network architectures. In this presentation, we will describe the basic architecture of SpF as well as preliminary performance data and experience with adapting legacy dynamo codes. We will conclude with a discussion of planned extensions to SpF that will provide pseudospectral applications with additional flexibility with regard to time integration, linear solvers, and discretization in the radial direction.

  5. Fuels irradiation testing for the SP-100 program

    NASA Technical Reports Server (NTRS)

    Makenas, Bruce J.; Hales, Janell W.; Ward, Alva L.

    1991-01-01

    An SP-100 fuel pin irradiation testing program is well on the way to providing data for performance correlations and demonstrating the lifetime and safety of the fuel system of the compact lithium-cooled reactor. Key SP-100 fuel performance issues addressed are the need for low fuel swelling and low fission gas release to minimize cladding strain, and the need for barrier integrity to prevent fuel/cladding chemical interaction. This paper provides a description of the irradiation test program that addresses these key issues and summarizes recent results of posttest examinations including data obtained at 6 atom percent goal burnup.

  6. SP-100 advanced radiator designs for thermoelectric and Stirling applications

    NASA Technical Reports Server (NTRS)

    Moriarty, M. P.; Determan, W. R.

    1989-01-01

    Advanced radiator designs employing carbon-carbon liquid metal heat pipe technology, which significantly reduce the mass of the heat rejection subsystem for high temperature space technology systems such as the SP-100 are discussed. This technology is being developed to address the need for lightweight heat transfer components and structures for space applications. Heat pipe and subsystem designs were optimized for thermoelectric- and Stirling-engine-based SP-100 system designs. A multiple, deployed-petal radiator concept was selected for the heat rejection subsystem design as it provided minimum mass. Radiator stowage in the space transportation system cargo bay and deployment schemes were investigated for each of the optimized designs.

  7. Natural infection by Giardia sp. and Cryptosporidium sp. in dairy goats, associated with possible risk factors of the studied properties.

    PubMed

    Bomfim, T C B; Huber, F; Gomes, R S; Alves, L L

    2005-11-25

    Visits were made to six farms raising dairy goats located in the mountain region of the state of Rio de Janeiro, Brazil, seeking to identify parasitism by Giardia sp. and Cryptosporidium sp. During the visits, fecal samples were collected from approximately 10% of the stock of each property. A questionnaire was given to the keepers on each property to obtain data for epidemiological analysis. A total of 105 fecal samples was collected, 56 from adult animals (over 12 months of age) and 49 from juvenile animals (less than 12 months). The fecal material was processed and subjected to the centrifuge-flotation technique and to staining with safranine-methylene blue. Protozoans of the genus Cryptosporidium were found at two properties, where the hygiene conditions of the installations were considered average and the stalls were made of wood slats raised from the ground. A total of five (4.8%) of the samples was found to be positive for the presence of this protozoan, all from juvenile animals. Cysts of the genus Giardia were found at two properties. Of the 105 samples analyzed, the protozoan was detected in 15 (14.3%), all in juvenile animals, and animals in the age range of 1-3 months had significantly more infections. Age, sanitary condition of the stalls and stalls made of wood slats and raised from the ground, can be pointed out as possible risk factors for infection by Giardia sp. and Cryptosporidium sp. The study reports for the first time the occurrence of Cryptosporidium sp. and Giardia sp. in goats in the state of Rio de Janeiro. PMID:16105719

  8. Fusarium euwallaceae sp. nov.--a symbiotic fungus of Euwallacea sp., an invasive ambrosia beetle in Israel and California.

    PubMed

    Freeman, S; Sharon, M; Maymon, M; Mendel, Z; Protasov, A; Aoki, T; Eskalen, A; O'Donnell, K

    2013-01-01

    The invasive Asian ambrosia beetle Euwallacea sp. (Coleoptera, Scolytinae, Xyleborini) and a novel Fusarium sp. that it farms in its galleries as a source of nutrition causes serious damage to more than 20 species of live trees and pose a serious threat to avocado production (Persea americana) in Israel and California. Adult female beetles are equipped with mandibular mycangia in which its fungal symbiont is transported within and from the natal galleries. Damage caused to the xylem is associated with disease symptoms that include sugar or gum exudates, dieback, wilt and ultimately host tree mortality. In 2012 the beetle was recorded on more than 200 and 20 different urban landscape species in southern California and Israel respectively. Euwallacea sp. and its symbiont are closely related to the tea shot-hole borer (E. fornicatus) and its obligate symbiont, F. ambrosium occurring in Sri Lanka and India. To distinguish these beetles, hereafter the unnamed xyleborine in Israel and California will be referred to as Euwallacea sp. IS/CA. Both fusaria exhibit distinctive ecologies and produce clavate macroconidia, which we think might represent an adaption to the species-specific beetle partner. Both fusaria comprise a genealogically exclusive lineage within Clade 3 of the Fusarium solani species complex (FSSC) that can be differentiated with arbitrarily primed PCR. Currently these fusaria can be distinguished only phenotypically by the abundant production of blue to brownish macroconidia in the symbiont of Euwallacea sp. IS/CA and their rarity or absence in F. ambrosium. We speculate that obligate symbiosis of Euwallacea and Fusarium, might have driven ecological speciation in these mutualists. Thus, the purpose of this paper is to describe and illustrate the novel, economically destructive avocado pathogen as Fusarium euwallaceae sp. nov. S. Freeman et al. PMID:23928415

  9. Rhodovulum salis sp. nov. and Rhodovulum viride sp. nov., phototrophic Alphaproteobacteria isolated from marine habitats.

    PubMed

    Srinivas, A; Vinay Kumar, B; Divya Sree, B; Tushar, L; Sasikala, Ch; Ramana, Ch V

    2014-03-01

    Two strains (JA746(T) and JA756(T)) having yellowish brown-to-green pigment were isolated from a solar saltern and a pink pond, respectively. While both strains were non-motile and shared the presence of bacteriochlorophyll-a, major cellular fatty acids (C18?:?1?7c, C16?:?0, C18?:?0), quinone (Q-10), polar lipids and hopanoids, they differed from each other in their carotenoid composition. The G+C content of genomic DNA of strains JA746(T) and 756(T) was 62.4 and 63.3 mol%, respectively. The 16S rRNA gene-based EzTaxon-e blast search analysis of strains JA746(T) and 756(T) indicated highest sequence similarity with members of the genus Rhodovulum in the family Rhodobacteraceae of the class Alphaproteobacteria. Strain JA746(T) has high sequence similarities with Rhodovulum visakhapatnamense JA181(T) (97.3?%), Rhodovulum steppense A-20s(T) (97.3?%), Rhodovulum phaeolacus JA580(T) (97?%), Rhodovulum strictum MB-G2(T) (97?%) and other members of the genus Rhodovulum (<97?%). Strain JA756(T) has high sequence similarities with Rhodovulum visakhapatnamense JA181(T) (99.8?%), Rhodovulum sulfidophilum Hansen W4(T) (99.1?%), Rhodovulum kholense JA297(T) (97.9?%) and other members of the genus Rhodovulum (<97?%). The sequence similarity between strains JA746(T) and JA756(T) was 97.5?%. However, these strains are not closely related to each other or to their phylogenetic neighbours since the DNA-DNA reassociation values were less than 56?%. The genomic information was also supported by phenotypic and chemotaxonomic results, leading us to classify strains JA746(T) (?=?NBRC 108898(T)?=?KCTC 15180(T)) and JA756(T) (?=?NBRC 109122(T)?=?KCTC 15223(T)) as the type strains of two novel species of the genus Rhodovulum, for which the names Rhodovulum salis sp. nov. and Rhodovulum viride sp. nov. are proposed, respectively. PMID:24425825

  10. Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant.

    PubMed

    Singh, Hina; Du, Juan; Ngo, Hien T T; Won, KyungHwa; Yang, Jung-Eun; Kim, Ki-Young; Yi, Tae-Hoo

    2015-06-01

    Two bacterial strains, designated THG-DN8.7(T) and THG-DN8.3(T), were isolated from the rhizosphere of a strawberry plant in Gyeryong Mountain, South Korea. Cells of both isolates were observed to be Gram-negative, yellow-coloured and rod-shaped. Comparative 16S rRNA gene sequence analysis showed that strain THG-DN8.7(T) had highest sequence similarities to Lysobacter yangpyeongensis KACC 11407(T) (97.2 %), Lysobacter niabensis KACC 11587(T) (97.0 %) and Lysobacter oryzae KCTC 22249(T) (96.9 %), while strain THG-DN8.3(T) had closely similarity with L. niabensis KACC 11587(T) (98.1 %), L. oryzae KCTC 22249(T) (97.1 %) and L. yangpyeongensis KACC 11407(T) (96.1 %). DNA-DNA relatedness values between strains THG-DN8.7(T) and THG-DN8.3(T) and their closest phylogenetically neighbours were below 30.0 %, which indicates that strains THG-DN8.7(T) and THG-DN8.3(T) represent distinct species within the genus Lysobacter. Both strains were found to contain iso-C15:0, iso-C16:0 and iso-C17:1 ?9c as predominant fatty acids and ubiquinone-8 as major isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strains THG-DN8.7(T) and THG-DN8.3(T) were determined to be 66.9 and 67.8 mol%, respectively. These data are consistent with the affiliation of the two new species represented by THG-DN8.7(T) and THG-DN8.3(T) to the genus Lysobacter. The names Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. are proposed for these species with the type strains THG-DN8.7(T) (=KCTC 42236(T) = JCM 30322(T)) and THG-DN8.3(T) (=KCTC 42237(T) = JCM 30321(T)), respectively. PMID:25828767

  11. Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., Isolated from Sea Water

    PubMed Central

    Ng, Hooi Jun; López-Pérez, Mario; Webb, Hayden K.; Gomez, Daniela; Sawabe, Tomoo; Ryan, Jason; Vyssotski, Mikhail; Bizet, Chantal; Malherbe, François; Mikhailov, Valery V.; Crawford, Russell J.; Ivanova, Elena P.

    2014-01-01

    Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1T and A3d10T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4°C and 40°C, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C16:0, C16:1?7c, C18:1?9c and C18:1?7c. The G+C content of the DNA for strains R9SW1T and A3d10T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved protein-coding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1T clusters with M. algicola DG893T sharing 99.40%, and A3d10T clusters with M. sediminum R65T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1T and A3d10T represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1T (?=? LMG 27497T ?=? JCM 19399T ?=? CIP 110588T ?=? KMM 7502T) and Marinobacter similis sp. nov., with the type strain A3d10T (?=? JCM 19398T ?=? CIP 110589T ?=? KMM 7501T), are proposed. PMID:25198502

  12. Sphingomonas aerophila sp. nov. and Sphingomonas naasensis sp. nov., isolated from air and soil, respectively.

    PubMed

    Kim, Soo-Jin; Moon, Ji-Young; Lim, Jun-Muk; Ahn, Jae-Hyung; Weon, Hang-Yeon; Ahn, Tae-Young; Kwon, Soon-Wo

    2014-03-01

    Two strains, designated 5413J-26(T) and KIS18-15(T), were isolated from the air and forest soil, respectively, in South Korea. Cells of the two strains were Gram-stain-negative, aerobic, polar-flagellated and rod-shaped. According to the phylogenetic tree, strains 5413J-26(T) and KIS18-15(T) fell into the cluster of Sphingomonas sensu stricto. Strain 5413J-26(T) showed the highest sequence similarities with Sphingomonas trueperi LMG 2142(T) (96.6%), Sphingomonas molluscorum KMM 3882(T) (96.5%), Sphingomonas azotifigens NBRC 15497(T) (96.3?%) and Sphingomonas pituitosa EDIV(T) (96.1?%), while strain KIS18-15(T) had the highest sequence similarity with Sphingomonas soli T5-04(T) (96.8%), Sphingomonas pituitosa EDIV(T) (96.6%), Sphingomonas leidyi ATCC 15260(T) (96.6?%), Sphingomonas asaccharolytica NBRC 15499(T) (96.6?%) and Sphingomonas koreensis JSS26(T) (96.6?%). The 16S rRNA gene sequence similarity between strains 5413J-26(T) and KIS18-15(T) was 95.4?%. Ubiquinone 10 was the predominant respiratory quinone and homospermidine was the major polyamine. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and several unidentified phospholipids and lipids. The main cellular fatty acids (>10% of the total fatty acids) of strain 5413J-26(T) were summed feature 8 (C18?:?1?6c and/or C18?:?1?7c), summed feature 3 (C16?:?1?7c and/or iso-C15?:?0 2-OH) and C14?:?0 2-OH, and those of strain KIS18-15(T) were summed feature 8 and C16?:?0. Based on the results of 16S rRNA gene sequence analysis, and physiological and biochemical characterization, two novel species with the suggested names Sphingomonas aerophila sp. nov. (type strain 5413J-26(T)?=?KACC 16533(T)?=?NBRC 108942(T)) and Sphingomonas naasensis sp. nov. (type strain KIS18-15(T)?=?KACC 16534(T)?=?NBRC 108943(T)) are proposed. PMID:24425812

  13. Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., isolated from sea water.

    PubMed

    Ng, Hooi Jun; López-Pérez, Mario; Webb, Hayden K; Gomez, Daniela; Sawabe, Tomoo; Ryan, Jason; Vyssotski, Mikhail; Bizet, Chantal; Malherbe, François; Mikhailov, Valery V; Crawford, Russell J; Ivanova, Elena P

    2014-01-01

    Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1T and A3d10T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4 °C and 40 °C, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C16:0, C16:1?7c, C18:1?9c and C18:1?7c. The G+C content of the DNA for strains R9SW1T and A3d10T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved protein-coding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1T clusters with M. algicola DG893T sharing 99.40%, and A3d10T clusters with M. sediminum R65T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1T and A3d10(T) represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1(T) (?=? LMG 27497(T) ?=? JCM 19399(T) ?=? CIP 110588(T) ?=? KMM 7502(T)) and Marinobacter similis sp. nov., with the type strain A3d10(T) (?=? JCM 19398(T) ?=? CIP 110589(T) ?=? KMM 7501T), are proposed. PMID:25198502

  14. 40 CFR 180.1075 - Colletotrichum gloeosporioides f. sp. aeschynomene; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...2010-07-01 2010-07-01 false Colletotrichum gloeosporioides f. sp. aeschynomene...Exemptions From Tolerances § 180.1075 Colletotrichum gloeosporioides f. sp. aeschynomene...established for residues of the mycoherbicide Colletotrichum gloeosporioides f. sp....

  15. Descriptions of Chitwoodius brasiliensis n.sp., Chitwoodius rusticulus n.sp., and Vancterlindia venata n.sp. (Dorylaimida: Tylencholaimidae: Vanderlindiinae)

    PubMed Central

    Siddiqi, Mohammad Rafiq

    1983-01-01

    Chitwoodius brasiliensis n.sp. is described from soil around plant roots in Vicosa, MG State, Brazil. It has a body 1.9 mm (1.6-2.5) long, odontostyle and odontophore 33 ?m (31-37) and 34 ?m (31-36) long, respectively, and males with spicules 59-61 ?m long. Chitwoodius rusticulus n.sp. from Colombian rain forest differs from other species of the genus in having a weakly muscular anterior part of the oesophagus, a pore-like vulva, and an unsclerotized vagina. Vanderlindia venata n.sp. from lucerne soil in South Kilimanjaro, Tanzania, differs from the type and only other known species of its genus in having a smaller and less slender body (L = 3.34 mm [3.15-3.71], a = 50 [46-53]), odontostyle 2.3-2.5 times lip region width long, and stylet guiding ring located at 1.2-1.4 times lip region width from anterior end. PMID:19295790

  16. Pedobacter nyackensis sp. nov., Pedobacter alluvionis sp. nov. and Pedobacter borealis sp. nov., isolated from Montana flood-plain sediment and forest soil.

    PubMed

    Gordon, Nathan S; Valenzuela, Alejandra; Adams, Sandra M; Ramsey, Philip W; Pollock, Jarrod L; Holben, William E; Gannon, James E

    2009-07-01

    Three Gram-negative, rod-shaped, non-spore-forming eubacterial strains were isolated in western Montana, USA, and subjected to taxonomic studies. Strains NWG-II14(T) and NWER-II11(T) were isolated from hyporheic sediments of a large alluvial flood plain, whereas strain G-1(T) was isolated from a conifer forest soil. On the basis of 16S rRNA gene sequence similarity, strains NWG-II14(T), NWER-II11(T) and G-1(T) were shown to belong to the family Sphingobacteriaceae and are most closely related to various species of the genus Pedobacter. The results of molecular, physiological and biochemical tests allowed genotypic and phenotypic differentiation of these three strains from 23 Pedobacter species with validly published names. The three isolates therefore represent novel species, for which the names Pedobacter nyackensis sp. nov. (type strain NWG-II14(T) =DSM 19625(T) =LMG 24260(T)), Pedobacter alluvionis sp. nov. (type strain NWER-II11(T) =DSM 19624(T) =LMG 24258(T)) and Pedobacter borealis sp. nov. (type strain G-1(T) =DSM 19626(T) =LMG 24259(T)) are proposed. PMID:19542109

  17. Supporting Information Geobacter sp. SD-1 with enhanced electrochemical activity in high salt

    E-print Network

    1 Supporting Information Geobacter sp. SD-1 with enhanced electrochemical activity in high salt title: Geobacter sp. SD-1 in high salt solutions #12;2 Fig. S1. Current generation as a function of time

  18. An Insight into the Diverse Roles of Surfactant Proteins, SP-A and SP-D in Innate and Adaptive Immunity

    PubMed Central

    Nayak, Annapurna; Dodagatta-Marri, Eswari; Tsolaki, Anthony George; Kishore, Uday

    2012-01-01

    Surfactant proteins SP-A and SP-D are hydrophilic, collagen-containing calcium-dependent lectins, which appear to have a range of innate immune functions at pulmonary as well as extrapulmonary sites. These proteins bind to target ligands on pathogens, allergens, and apoptotic cells, via C-terminal homotrimeric carbohydrate recognition domains, while the collagen region brings about the effector functions via its interaction with cell surface receptors. SP-A and SP-D deal with various pathogens, using a range of innate immune mechanisms such as agglutination/aggregation, enhancement of phagocytosis, and killing mechanisms by phagocytic cells and direct growth inhibition. SP-A and SP-D have also been shown to be involved in the control of pulmonary inflammation including allergy and asthma. Emerging evidence suggest that SP-A and SP-D are capable of linking innate immunity with adaptive immunity that includes modulation of dendritic cell function and helper T cell polarization. This review enumerates immunological properties of SP-A and SP-D inside and outside lungs and discusses their importance in human health and disease. PMID:22701116

  19. Expression of lung surfactant proteins SP-B and SP-C and their modulating factors in fetal lung of FGR rats.

    PubMed

    Deng, Fei-tao; Ouyang, Wei-xiang; Ge, Liang-fang; Zhang, Li; Chai, Xin-qun

    2015-02-01

    This study investigated the expression of lung surfactant proteins SP-B and SP-C, and their modulating factors TTF-1 and PLAGL2 in the fetal lung of rats with fetal growth restriction (FGR). The rat FGR model was established by prenatal hypoxia in the first stage of pregnancy, 180 rats for experiment served as hypoxia group, and 197 healthy rats served as normal control group. The FGR incidence in hypoxia was compared with that in normal control group. The histological changes in the fetal lung were observed under the light microscope and electronic microscope in two groups. The SP-B, SP-C, TTF-1 and PLAGL2 proteins were determined in the fetal lung of two groups immunohistochemically. The expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and mRNA in the fetal lung of two groups were detected by using Western blotting and RT-PCR respectively. The FGR rat model was successfully established by using hypoxia. Pathologically the fetal lung developed slowly, and the expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and mRNA in the fetal lung were significantly reduced in hypoxia group as compared with those in normal control group. It was suggested that maternal hypoxia in the first stage of pregnancy could induce FGR, and reduce the expression of SP-B and SP-C, resulting in the disorder of fetal lung development and maturation. PMID:25673205

  20. Enantiospecific sp(2)-sp(3) coupling of secondary and tertiary boronic esters.

    PubMed

    Bonet, Amadeu; Odachowski, Marcin; Leonori, Daniele; Essafi, Stephanie; Aggarwal, Varinder K

    2014-07-01

    The cross-coupling of boronic acids and related derivatives with sp(2) electrophiles (the Suzuki-Miyaura reaction) is one of the most powerful C-C bond formation reactions in synthesis, with applications that span pharmaceuticals, agrochemicals and high-tech materials. Despite the breadth of its utility, the scope of this Nobel prize-winning reaction is rather limited when applied to aliphatic boronic esters. Primary organoboron reagents work well, but secondary and tertiary boronic esters do not (apart from a few specific and isolated examples). Through an alternative strategy, which does not involve using transition metals, we have discovered that enantioenriched secondary and tertiary boronic esters can be coupled to electron-rich aromatics with essentially complete enantiospecificity. As the enantioenriched boronic esters are easily accessible, this reaction should find considerable application, particularly in the pharmaceutical industry where there is growing awareness of the importance of, and greater clinical success in, creating biomolecules with three-dimensional architectures. PMID:24950327

  1. Mycetophyllia sp. Coral Abundance in the Virgin Islands

    USGS Multimedia Gallery

    This coral (Mycetophyllia sp.) is not abundant in coral reefs of the Virgin Islands. It most commonly grows in water deeper than 40 ft., and it is surprising to find it growing in the shade of the mangroves in 3 ft of water....

  2. NASA/SP-2007-6105 Systems Engineering

    E-print Network

    Rhoads, James

    its founding, the National Aeronautics and Space Administration (NASA) has been dedicated to the ad 21076-1320 #12;NASA/SP-2007-6105 Rev1 Systems Engineering Handbook National Aeronautics and Space- vancement of aeronautics and space science. The NASA Scientific and Technical Information (STI) program

  3. Draft Genome of the Arthrobacter sp. Strain Edens01

    PubMed Central

    Couger, M. B.; Hanafy, Radwa A.; Edens, Curtis; Budd, Connie; French, Donald P.; Hoff, Wouter D.; Elshahed, Mostafa S.

    2015-01-01

    We report the draft genome sequence of Arthrobacter sp. strain Edens01, isolated from a leaf surface of a Rosa hybrid plant as part of the Howard Hughes Medical Institute-funded Student Initiated Microbial Discovery (SIMD) project. The genome has a total size of 3,639,179 bp and contig N50 of 454,897 bp. PMID:26679586

  4. Carbon films with an sp2 network structure

    E-print Network

    Powles, Rebecca

    as leading to the formation of soft films. However, the discovery of the C60 fullerene molecule3,4 and carbon, which exploit the extremely strong6 in-planar bonds of graphite stronger than diamond in a new class of hard thin-film materials. The reports of the formation of hard sp2 -bonded materials by anisotropically

  5. A thermostable cyclodextrin glycosyltransferase from Thermoanaerobacter sp. 5K

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cyclodextrin glycosyltransferase (CGTase) from the thermophilic anaerobe Thermoanaerobacter sp. 5K was purified and characterized. The enzyme was purified with ammonium sulfate precipitation followed by a-CD-bound, epoxy-activated Sepharose 6B affinity chromatography. Molecular weight of the purifie...

  6. Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2

    PubMed Central

    Xu, Hui; Han, Dongmei; Xu, Zhaohui

    2015-01-01

    The ability of Thermotoga spp. to degrade cellulose is limited due to a lack of exoglucanases. To address this deficiency, cellulase genes Csac_1076 (celA) and Csac_1078 (celB) from Caldicellulosiruptor saccharolyticus were cloned into T. sp. strain RQ2 for heterologous overexpression. Coding regions of Csac_1076 and Csac_1078 were fused to the signal peptide of TM1840 (amyA) and TM0070 (xynB), resulting in three chimeric enzymes, namely, TM1840-Csac_1078, TM0070-Csac_1078, and TM0070-Csac_1076, which were carried by Thermotoga-E. coli shuttle vectors pHX02, pHX04, and pHX07, respectively. All three recombinant enzymes were successfully expressed in E. coli DH5? and T. sp. strain RQ2, rendering the hosts with increased endo- and/or exoglucanase activities. In E. coli, the recombinant enzymes were mainly bound to the bacterial cells, whereas in T. sp. strain RQ2, about half of the enzyme activities were observed in the culture supernatants. However, the cellulase activities were lost in T. sp. strain RQ2 after three consecutive transfers. Nevertheless, this is the first time heterologous genes bigger than 1?kb (up to 5.3?kb in this study) have ever been expressed in Thermotoga, demonstrating the feasibility of using engineered Thermotoga spp. for efficient cellulose utilization. PMID:26273605

  7. Polydactyly in the central pacific gecko, Lepidodactylus sp. (Squamata: Gekkonidae)

    USGS Publications Warehouse

    Bauer, A.M.; Hathaway, S.A.; Fisher, R.N.

    2009-01-01

    We report the first known case of naturally occurring polydactyly in a gekkotan lizard. A single individual from Palmyra Atoll exhibited a triplication of digit III of the m hand. No obvious teratogenic sources are present on the atoll and the causal factors of polydactyly in Lepidodactylus sp. remain unknown.

  8. Sphaeromyxa cannolii n.sp. (Myxosporea: Spheromyxidae) a

    E-print Network

    Kane, Andrew S.

    · Measured with ImageJ · TEM 5 TEM Results: Sphaeromyxa sp. 6 #12;4 Results · Spores ­ 17.56 ± 0.20 µm long by sex: · Females: 11/28 = 39.3% · Males: 0/16 fishindex.blogspot.comfishindex.blogspot.com 9 Remarks. Holloway, and J. Miller: photography, dissections ­K. Kelley: TEM images · Florida Aquarium ­P. Perkins

  9. Description of Nemophora acaciae sp. nov. (Lepidoptera: Adelidae) from Kenya.

    PubMed

    Agassiz, David J L; Kozlov, Mikhail V

    2015-01-01

    Nemophora acaciae sp. nov. is described from Kenya on the basis of a large series bred from flowers of Acacia seyal and A. lahai. The new species differs from all Afrotropical Nemophora species by its dark brown forewing fascia with white medial stripe near the costal margin of forewing. The key to the Afrotropical Nemophora species is provided. PMID:26701526

  10. Blastopirellula cremea sp. nov., isolated from a dead ark clam

    E-print Network

    Bae, Jin-Woo

    Blastopirellula cremea sp. nov., isolated from a dead ark clam Hae-Won Lee,1, 2 3 Seong Woon Roh,1 a dead ark clam (Scapharca broughtonii) from the south coast of Korea. Strain LHWP2T grew optimally at 30 from a dead ark clam (Scapharca broughtonii) collected from a cage-cultured ark clam farm in Gangjin

  11. Draft Genome Sequence of Linfuranone Producer Microbispora sp. GMKU 363.

    PubMed

    Komaki, Hisayuki; Ichikawa, Natsuko; Hosoyama, Akira; Fujita, Nobuyuki; Thamchaipenet, Arinthip; Igarashi, Yasuhiro

    2015-01-01

    Here, we report the draft genome sequence of Microbispora sp. GMKU 363, a plant-derived actinomycete that produces linfuranone A, a linear polyketide modified with a furanone ring possessing adipocyte differentiation inducing activity. The biosynthetic gene cluster for linfuranone was identified by analyzing polyketide synthase genes in the genome. PMID:26659694

  12. Pseudomonas sabulinigri sp. nov., isolated from black beach sand

    E-print Network

    Bae, Jin-Woo

    Pseudomonas sabulinigri sp. nov., isolated from black beach sand Kyoung-Ho Kim,1 Seong Woon Roh,1 , was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Cells grew at 4­37 6C, at pH 5 beach sand, a bacterium was isolated and subjected to taxonomic characterization. On the basis

  13. Soil treatments against Fusarium oxysporum f. sp. vasinfectum race 4

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Few economically feasible disease management options are available for California cotton producers with fields infested with race 4 of Fusarium oxysporum f. sp. vasinfectum. For treating soil to reduce inoculum levels, past studies indicate that solarization and fumigation with metam-sodium may be a...

  14. Agromyces atrinae sp. nov., isolated from fermented seafood

    E-print Network

    Bae, Jin-Woo

    Agromyces atrinae sp. nov., isolated from fermented seafood Eun-Jin Park,1 Min-Soo Kim,1,2 Mi, designated P27T , was isolated from a traditional fermented seafood. The isolate grew optimally with 0 a traditional fermented seafood that is generally made with plenty of salt. Strain P27T was isolated using

  15. Kocuria salsicia sp. nov., isolated from salt-fermented seafood

    E-print Network

    Bae, Jin-Woo

    Kocuria salsicia sp. nov., isolated from salt- fermented seafood Ji-Hyun Yun,1 Seong Woon Roh,1 salt-fermented seafood in Korea. It was a Gram- positive, non-motile, coccus-shaped bacterium sediment (Kim et al., 2004), saline desert soil (Li et al., 2006), fermented food (Park et al., 2010a, b

  16. Brachybacterium squillarum sp. nov., isolated from salt-fermented seafood

    E-print Network

    Bae, Jin-Woo

    Brachybacterium squillarum sp. nov., isolated from salt-fermented seafood Seong-Kyu Park, Min salt-fermented seafood in Korea. The organism grew in 0­10 % (w/v) NaCl and at 25­37 6C, with optimal et al., 2007). While carrying out a study examining the microbial diversity in salt-fermented food

  17. Lactobacillus kimchiensis sp. nov., isolated from a fermented food

    E-print Network

    Bae, Jin-Woo

    Lactobacillus kimchiensis sp. nov., isolated from a fermented food Jandi Kim,3 Joon Yong Kim,3 Min bacterium was isolated from a traditional fermented food, kimchi. The morphology, physiology, biochemical ). Kimchi is a traditional Korean fermented food made of various vegetables and spices (red pepper, garlic

  18. Kocuria atrinae sp. nov., isolated from traditional Korean fermented seafood

    E-print Network

    Bae, Jin-Woo

    Kocuria atrinae sp. nov., isolated from traditional Korean fermented seafood Eun-Jin Park, Min fermented seafood. Cells were aerobic, Gram-positive, non-motile and coccoid. Optimal growth occurred at 30 of narrow- leaved cattail, a cold desert soil of the Indian Himalayas and fermented seafood (Stackebrandt et

  19. Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter

    E-print Network

    Bae, Jin-Woo

    Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter Na-Ri Shin, Mi , was isolated from a Korean alcohol fermentation starter. Optimal growth occurred at 37 6C, at pH 8 and in 1 was isolated from nuruk, which is an alcohol fermentation starter used as an amylolytic substance

  20. Leucobacter celer sp. nov., isolated from Korean fermented seafood

    E-print Network

    Bae, Jin-Woo

    Leucobacter celer sp. nov., isolated from Korean fermented seafood Na-Ri Shin, Min-Soo Kim, Mi, designated NAL101T , was isolated from gajami-sikhae, a traditional Korean fermented seafood made of flatfish ). Sikhae, a kind of traditional Korean food, also known as jeotgal, consists of fermented fish without

  1. Leucobacter salsicius sp. nov., from a salt-fermented food

    E-print Network

    Bae, Jin-Woo

    Leucobacter salsicius sp. nov., from a salt- fermented food Ji-Hyun Yun,1 Seong Woon Roh,1,2 Min, Daejeon 305-806, Republic of Korea Strain M1-8T was isolated from jeotgal, a Korean salt-fermented food salt-fermented seafood. Shrimp jeotgal is made by mixing fresh tiny shrimps with rock salt, followed

  2. CriSP a Tool for Bump Hunting Jaroslaw Harezlak

    E-print Network

    Heckman, Nancy E.

    CriSP ­ a Tool for Bump Hunting Jaroslaw Harezlak Indiana University School of Medicine (1971). In the 1980's nonparametric methods in bump­hunting started gaining popularity due to greater) and Heckman (1992) discussed the issues involved with bump hunting in regression but without giving

  3. NATURAL BESNOITIA SP. INFECTION IN DOMESTIC RABBITS FROM ARGENTINA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Besnoitia spp. are apicomplexan coccidian parasites affecting several species of mammals and cold-blooded animals in several countries. Besnoitia sp. tissue cysts were seen in several tissues of five rabbits from a rabbit breeder in La Plata, Argentina. Bradyzoites released from macroscopic tissue...

  4. Draft Genome Sequence of Linfuranone Producer Microbispora sp. GMKU 363

    PubMed Central

    Ichikawa, Natsuko; Hosoyama, Akira; Fujita, Nobuyuki; Thamchaipenet, Arinthip; Igarashi, Yasuhiro

    2015-01-01

    Here, we report the draft genome sequence of Microbispora sp. GMKU 363, a plant-derived actinomycete that produces linfuranone A, a linear polyketide modified with a furanone ring possessing adipocyte differentiation inducing activity. The biosynthetic gene cluster for linfuranone was identified by analyzing polyketide synthase genes in the genome. PMID:26659694

  5. Sphingomonas aestuarii sp. nov., isolated from tidal flat sediment

    E-print Network

    Bae, Jin-Woo

    Sphingomonas aestuarii sp. nov., isolated from tidal flat sediment Seong Woon Roh,1 Kyoung-Ho Kim,1. A novel bacterium (strain K4T ) belonging to the genus Sphingomonas was isolated from tidal flat sediment with the genus Sphingomonas. Within the phylogenetic tree, this novel strain shares a branching point

  6. THE SAGA OF A MALE FERTILITY PROTEIN (SP22)

    EPA Science Inventory

    Toxicologic studies designed to identify chemical-induced alterations in the structure and function of the epididymis, particularly the acquisition of fertility by proximal cauda epididymal sperm, have lead to the discovery of a novel sperm protein (SP22) that is well correlated ...

  7. [Cellulase activity of Ceratocystis sp. of different trophic groups].

    PubMed

    Kurchenko, I M; Sokolova, O V; Iur'ieva, O M; Zhdanova, N M

    2009-01-01

    A comparative analysis of cellulase activity of 36 fungal strains of phytopathogenic and endophytic Ceratocystis sp. was conducted. The rate of their linear growth on the media with carboxymethylcellulose was studied. It was shown that the rate of linear growth of phytopathogenic strains on the media with carboxymethylcellulose was lower than that in endophytic ones. There was no correlation between the levels of cellulase activity of studied strains and rates of their linear growth. The majority of Ceratocystis sp. strains had middle but not high cellulase activity, cellulase activity varied in the group of endophytic strains more than in the phytopathogenic one. The differences in cellulase activity were observed on the strain level. No distinct dependence of cellulase activity on different growth terms of fungi was demonstrated. The distinct dependence of cellulase activity level of endophytes on the species and organs of host plant was not observed. The cellulase activity level of endophytic strains Ceratocystis sp. was lower than in endophytic Fusarium poae strains and similar to it in phytopathogenic Fusarium strains. Consequently the investigated endophytic Ceratocystis sp. strains can be classified as latent pathogens, which were able to cause the diseases of host plants in favorable for them conditions. The paper is presented in Ukrainian. PMID:20455430

  8. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... AND WATERWAYS SAFETY VESSEL TRAFFIC MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15 minutes before navigating a VTS area, a vessel must report the: (a) Vessel name and type; (b) Position; (c) Destination and ETA; (d) Intended route; (e) Time...

  9. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... AND WATERWAYS SAFETY VESSEL TRAFFIC MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15 minutes before navigating a VTS area, a vessel must report the: (a) Vessel name and type; (b) Position; (c) Destination and ETA; (d) Intended route; (e) Time...

  10. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... AND WATERWAYS SAFETY VESSEL TRAFFIC MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15 minutes before navigating a VTS area, a vessel must report the: (a) Vessel name and type; (b) Position; (c) Destination and ETA; (d) Intended route; (e) Time...

  11. 33 CFR 161.19 - Sailing Plan (SP).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AND WATERWAYS SAFETY VESSEL TRAFFIC MANAGEMENT Vessel Movement Reporting System § 161.19 Sailing Plan (SP). Unless otherwise stated, at least 15 minutes before navigating a VTS area, a vessel must report the: (a) Vessel name and type; (b) Position; (c) Destination and ETA; (d) Intended route; (e) Time...

  12. ORIGINAL PAPER Candida gelsemii sp. nov., a yeast

    E-print Network

    Thomson, James D.

    ORIGINAL PAPER Candida gelsemii sp. nov., a yeast of the Metschnikowiaceae clade isolated from+Business Media B.V. 2006 Abstract A new yeast species, Candida gelsemii, is described to accommodate three Metschnikowiaceae Á Gelsemium sempervirens Á Nectar alkaloids Á Gelsemine Á New yeast species Introduction Floral

  13. Civilian and military missions SP-100 preliminary user requirements

    SciTech Connect

    Not Available

    1987-06-29

    This document defines the top level requirements of potential users of a space based nuclear electric power supply. This provides the SP-100 Project and information required to design the modular (10-1000 KWe) space power systems to meet the needs of most potential users.

  14. FOOD FISH FACTS Sp4nlSh Mackerel

    E-print Network

    46 FOOD FISH FACTS Sp4nlSh Mackerel (Scomberomorus m~cul.1lw) Spanish mackerel are members of a large family of fish that include the tunas and other mackerels. All of these fish, although vary- ing of this family live in the open sea and are more or less migratory. Spanish mackerel are known

  15. Oil from the tropical marine benthic-diatom Navicula sp.

    PubMed

    Nurachman, Zeily; Brataningtyas, Dewi Susan; Hartati; Panggabean, Lily Maria Goretty

    2012-11-01

    The potential of the tropical marine benthic-diatom Navicula sp. for biodiesel feedstock was investigated. Growth profiles were analyzed by changing nutrient compositions in three different media (Walne, plain seawater, and modified seawater) and irradiance intensities. Navicula sp. cells showed significant growth in Walne and modified seawater medium but not in plain seawater medium. The microalgae grew well in a pH range of 7.8-8.4, and the cells were very sensitive to the intensity of direct sunlight exposure. The average cell concentration obtained from the cultures in plain seawater, Walne, and modified seawater media at the beginning of the stationary phase was 0.70, 2.17, and 2.54 g/L, respectively. Electron spray ionization-ion trap-mass spectrometry showed that the triacylglycerols of the algae oil were identified as POP (palmitic-oleic-palmitic), POO (palmitic-oleic-oleic), and OOLn (oleic-oleic-linoleic). The oil productivity of Navicula sp. cultivated in Walne and modified seawater media was 90 and 124 ?L L(-1) culture d(-1). The Navicula sp. biodiesel exhibited a kinematic viscosity of 1.299 mm(2)/s, density of 0.8347 g/mL, and internal energy of 0.90 kJ/mL. PMID:22926527

  16. Sequence of ornithine decarboxylase from Lactobacillus sp. strain 30a.

    PubMed Central

    Hackert, M L; Carroll, D W; Davidson, L; Kim, S O; Momany, C; Vaaler, G L; Zhang, L

    1994-01-01

    A gene encoding biodegradative ornithine decarboxylase from Lactobacillus sp. strain 30a was isolated from a genomic DNA library and sequenced. Primer extension analysis revealed two transcription initiation sites. The deduced amino acid sequence is compared with the amino acid sequences of five previously reported bacterial decarboxylases, and conserved pyridoxal phosphate motif residues are identified. PMID:7961515

  17. SOLID SUBSTRATE PRODUCTION OF ALTERNARIA ALTERNATA F. SP. SPHENOCLEAE.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sphenoclea zeyanica (gooseweed), a major weed of paddy rice in Southeast Asia, is one of the targets in a biological weed control research program in the Philippines. Afungal pathogen, Alternaria alternata f. sp. sphenocleae, is being evaluated as a biological control agent for this weed. The feas...

  18. Cp*Rh(III) -Catalyzed Arylation of C(sp(3) )-H Bonds.

    PubMed

    Wang, Xiaoming; Yu, Da-Gang; Glorius, Frank

    2015-08-24

    The first Cp*Rh(III) -catalyzed arylation of unactivated C(sp(3) )-H bonds is presented. The unactivated primary C(sp(3) )-H bond of 2-alkylpyridines can be activated by Rh(III) and further reacts with triarylboroxines to efficiently build new C(sp(3) )-aryl bonds. The methodology also provides a facile and efficient synthesis of unsymmetrical triarylmethanes by Rh(III) -catalyzed C(sp(3) )-H arylation of diarylmethanes. PMID:26095347

  19. Resistance to Puccinia graminis f. sp. avenae in Barley Is Associated with the Rpg5 Locus.

    PubMed

    Dracatos, Peter; Singh, Davinder; Fetch, Tom; Park, Robert

    2015-04-01

    In barley, gene Rpg5 was first identified for providing resistance to the rye stem rust pathogen (Puccinia graminis f. sp. secalis). A subsequent study determined that Rpg5 is required for rpg4-mediated resistance to the wheat stem rust pathogen (P. graminis f. sp. tritici) including pathotype TTKSK ("Ug99"), which poses a major threat to global wheat and barley production. Based on the effectiveness of Rpg5 against P. graminis f. sp. tritici and P. graminis f. sp. secalis, we assessed whether it also conferred resistance to the oat stem rust pathogen (P. graminis f. sp. avenae). A barley F8 recombinant inbred line (RIL) population was produced by crossing 'Q21861' (Rpg1 and Rpg5) with '73-G1' (Rpg1), which is susceptible to P. graminis f. sp. avenae, P. graminis f. sp. secalis, and some pathotypes of P. graminis f. sp. tritici. Seedling tests were performed on the F8 RIL population using Australian pathotypes of P. graminis f. sp. tritici, P. graminis f. sp. secalis, P. graminis f. sp. avenae, and a putative somatic hybrid between P. graminis f. sp. tritici and P. graminis f. sp. secalis known as the 'Scabrum' rust. Segregation in the responses to all rust isolates for the RILs was identical (50 resistant: 52 susceptible), and fitted a 1:1 ratio (X2=0.039, P=0.843), indicating that resistance to all isolates was monogenetically inherited. Screening of the RILs and the parental lines with perfect markers for the functional Rpg1 and Rpg5 resistance alleles indicated that Rpg1 was fixed, while Rpg5 was positive in all resistant lines and negative in all susceptible lines. This suggests that different formae speciales of P. graminis may share common effectors, and that the Rpg5 locus confers resistance to both P. graminis f. sp. tritici and P. graminis f. sp. secalis and the heterologous formae speciales of P. graminis, P. graminis f. sp. avenae. PMID:25870923

  20. Degradation of Phycobilisomes in Synechocystis sp. PCC6803

    PubMed Central

    Baier, Antje; Winkler, Wiebke; Korte, Thomas; Lockau, Wolfgang; Karradt, Anne

    2014-01-01

    When cyanobacteria acclimate to nitrogen deficiency, they degrade their large (3–5-MDa), light-harvesting complexes, the phycobilisomes. This massive, yet specific, intracellular degradation of the pigmented phycobiliproteins causes a color change of cyanobacterial cultures from blue-green to yellow-green, a process referred to as chlorosis or bleaching. Phycobilisome degradation is induced by expression of the nblA gene, which encodes a protein of ?7 kDa. NblA most likely acts as an adaptor protein that guides a Clp protease to the phycobiliproteins, thereby initiating the degradation process. Most cyanobacteria and red algae possess just one nblA-homologous gene. As an exception, the widely used “model organism” Synechocystis sp. PCC6803 expresses two such genes, nblA16803 and nblA26803, both of whose products are required for phycobilisome degradation. Here, we demonstrate that the two NblA proteins heterodimerize in vitro and in vivo using pull-down assays and a Förster energy-transfer approach, respectively. We further show that the NblA proteins form a ternary complex with ClpC (the HSP100 chaperone partner of Clp proteases) and phycobiliproteins in vitro. This complex is susceptible to ATP-dependent degradation by a Clp protease, a finding that supports a proposed mechanism of the degradation process. Expression of the single nblA gene encoded by the genome of the N2-fixing, filamentous cyanobacterium Nostoc sp. PCC7120 in the nblA1/nblA2 mutant of Synechocystis sp. PCC6803 induced phycobilisome degradation, suggesting that the function of the NblA heterodimer of Synechocystis sp. PCC6803 is combined in the homodimeric protein of Nostoc sp. PCC7120. PMID:24610785

  1. Synthesis of marine polyacetylenes callyberynes A-C by transition-metal-catalyzed cross-coupling reactions to sp centers.

    PubMed

    López, Susana; Fernandez-Trillo, Francisco; Midón, Pilar; Castedo, Luis; Saa, Carlos

    2006-03-31

    Efficient total syntheses of the sponge-derived hydrocarbon polyacetylenes callyberynes A-C have been achieved using metal-catalyzed cross-coupling reactions of highly unsaturated 1,3-diyne fragments as the key steps, namely: Cadiot-Chodkiewicz reaction under Alami's optimized conditions (sp-sp), sequential Sonogashira reaction of a cis,cis-divinyl dihalide (sp2-sp), and Kumada-Corriu reaction of an unactivated alkyl iodide (sp3-sp). This last approach constitutes the first application of a metal-catalyzed sp3-sp Kumada-Corriu cross-coupling reaction to the synthesis of a natural product. PMID:16555835

  2. Draft Genome Sequence of Rhodovulum sp. Strain NI22, a Naphthalene-Degrading Marine Bacterium

    PubMed Central

    Brown, Lisa M.; Gunasekera, Thusitha S.; Bowen, Loryn L.

    2015-01-01

    Rhodovulum sp. strain NI22 is a hydrocarbon-degrading member of the genus Rhodovulum. The draft genome of Rhodovulum sp. NI22 is 3.8 Mb in size, with 3,756 coding sequences and 64.4% G+C content. The catechol and gentisate pathways for naphthalene degradation are predicted to be present in Rhodovulum sp. NI22. PMID:25614575

  3. IDENTIFICATION AND MOLECULAR CLONING OF XENOPUS LAEVIS SP22, A PROTEIN ASSOCIATED WITH FERTILIZATION IN MAMMALS

    EPA Science Inventory

    ABSTRACT

    SP22 is a protein that has been characterized in rats where it has been related with fertility. SP22 homologues have been studied in mouse and man and a definitive role for the protein has not been assigned yet. By means of a polyclonal IgG to recombinant rat SP22...

  4. CU Recurring Pay for (SP) Summer Pay (SS) Summer School March 1, 2011 Page 1

    E-print Network

    Stuart, Steven J.

    CU Recurring Pay for (SP) Summer Pay (SS) Summer School March 1, 2011 Page 1 Log into People Pay · Type EmplID of the employee you are to enter Summer Pay (SP) or Summer School (SS) recurring. #12;CU Recurring Pay for (SP) Summer Pay (SS) Summer School March 1, 2011 Page 2 · Search

  5. 76 FR 72505 - Proposed Collection; Comment Request for Form 2290/SP/FR

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-23

    ...(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 2290/SP/FR Heavy Highway...: Heavy Highway Vehicle Use Tax Return. OMB Number: 1545-0143. Abstract: Form 2290/SP/FR is used to... Internal Revenue Service Proposed Collection; Comment Request for Form 2290/SP/FR AGENCY: Internal...

  6. An air itinerary choice model based on a mixed RP/SP dataset

    E-print Network

    Bierlaire, Michel

    An air itinerary choice model based on a mixed RP/SP dataset Bilge Atasoy Michel Bierlaire April/SP dataset. The aim of the combination of the two datasets is to exploit the variability of the SP data is modeled as a latent class. In this study we develop an itinerary choice model based on a real dataset

  7. Video streaming with SP and SI frames Eric Setton and Bernd Girod

    E-print Network

    Girod, Bernd

    Video streaming with SP and SI frames Eric Setton and Bernd Girod Information Systems Laboratory and delay on the congestion-rate-distortion performance of streaming with SI and SP frames. Our results help identify scenarios for which SI and SP frames provide an attractive alternative to streaming with I frames

  8. Whole-Genome Sequence of Enterobacter sp. Strain SST3, an Endophyte Isolated from Jamaican Sugarcane (Saccharum sp.) Stalk Tissue

    PubMed Central

    Gan, Han Ming; McGroty, Sean E.; Chew, Teong Han; Chan, Kok Gan; Buckley, Larry J.; Savka, Michael A.

    2012-01-01

    Enterobacter sp. strain SST3 is an endophytic bacterium isolated from Saccharum spp. Here we present its annotated draft genome that may shed light on its role as a bacterial endophyte of sugarcane. To our knowledge, this is the first genome announcement of a sugarcane-associated bacterium from the genus Enterobacter. PMID:23045495

  9. Gas Saturation and Sensitivity Analysis Using CRiSP 1 Gas Saturation and Sensitivity Analysis Using CRiSP

    E-print Network

    Washington at Seattle, University of

    or several of the dams would benefit fish survival. A gas sensitivity analysis was done using CRiSP to see of reducing gas at the region's top gas producing dams. In this analysis, steelhead and yearling chinook were released above Lower Granite pool and followed through the tailrace of Bonneville Dam. The runs were done

  10. Characterization and host range of the symbiotic fungus Fusarium euwallaceae sp. nov., vectored by the invasive ambrosia beetle Euwallacea sp.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A novel symbiotic Fusarium euwallaceae fungus that serves as a specific nutritional source for the invasive Asian ambrosia beetle Euwallacea sp. (Coleoptera, Scolytinae, Xyleborini) is farmed in the galleries of host plants. This beetle-fungus complex, which has invaded Israel and California, is clo...

  11. Mixibius parvus sp. nov. and Diphascon (Diphascon) ziliense sp. nov., two new species of Eutardigrada from Sicily.

    PubMed

    Lisi, Oscar; Sabella, Giorgio; Pilato, Giovanni

    2014-01-01

    Two new species, Mixibius parvus sp. nov. and Diphascon (Diphascon) ziliense sp. nov. are described from Sicily. Mixibius parvus sp. nov. has three macroplacoids and a microplacoid and differs from M. tibetanus, the only other known species of the genus with those characteristics, in having a wrinkled cuticle without true small tubercles, a shorter microplacoid, smaller claw pt index values, and in lacking a cuticular bar on the first three pairs of the legs.Diphascon (Diphascon) ziliense sp. nov. lacks eye spots, has a pharyngeal bulb with two macroplacoids and a septulum, and possesses lunules and cuticular bars on the legs. It is similar to D. (D.) ramazzottii and D. (D.) procerum but differs from them in characters of the cuticular ornamentation and, in addition, from D. (D.) ramazzottii in having lunules and slightly longer claws in proportion to the body size and to the buccal tube. The new species differs from D. (D.) procerum in having stouter claws with a wider common portion and with the main branches shorter in proportion to the total length of the respective claws. PMID:24871024

  12. Portal do Governo Cidado.SP Investimentos.SP Destaques: OK Secretaria do Ensino Superior do Estado

    E-print Network

    Barbosa, Marcia C. B.

    ://www.ensinosuperior.sp.gov.br/sis/lenoticia.ph... 1 de 4 24-07-2008 18:23 #12;Wrana Pazzini: distribuição desigual de sexo nas áreas de conhecimento. A coordenadora da mesa, Wrana Pazzini, lembrou ainda que a distribuição de sexo nas áreas de conhecimento são

  13. Role of Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 as Bioindicators and Immobilizers of Chromium in a Contaminated Natural Environment

    PubMed Central

    Millach, Laia; Solé, Antoni; Esteve, Isabel

    2015-01-01

    The aim of this work was to study the potential of the two phototrophic microorganisms, both isolated from Ebro Delta microbial mats, to be used as bioindicators and immobilizers of chromium. The results obtained indicated that (i) the Minimum Metal Concentration (MMC) significantly affecting Chlorophyll a intensity in Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 was 0.25?µM and 0.75?µM, respectively, these values being lower than those established by current legislation, and (ii) Scenedesmus sp. DE2009 was able to immobilize chromium externally in extracellular polymeric substances (EPS) and intracellularly in polyphosphate (PP) inclusions. Additionally, this microorganism maintained high viability, including at 500?µM. Based on these results, we postulate that Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 are good chromium-indicators of cytotoxicity and, further, that Scenedesmus sp. DE2009 plays an important role in immobilizing this metal in a contaminated natural environment. PMID:26167488

  14. Halobellus limi sp. nov. and Halobellus salinus sp. nov., isolated from two marine solar salterns.

    PubMed

    Cui, Heng-Lin; Yang, Xin; Zhou, Yu-Guang; Liu, Hong-Can; Zhou, Pei-Jin; Dyall-Smith, Mike L

    2012-06-01

    Two halophilic archaea, strains TBN53(T) and CSW2.24.4(T), were characterized to elucidate their taxonomic status. Strain TBN53(T) was isolated from the Taibei marine solar saltern near Lianyungang city, Jiangsu province, China, whereas strain CSW2.24.4(T) was isolated from a saltern crystallizer in Victoria, Australia. Cells of the two strains were pleomorphic, stained Gram-negative and produced red-pigmented colonies. Strain TBN53(T) was able to grow at 25-55 °C (optimum 45 °C), with 1.4-5.1 M NaCl (optimum 2.6-3.9 M NaCl), with 0-1.0 M MgCl(2) (optimum 0-0.1 M MgCl(2)) and at pH 5.5-9.5 (optimum pH 7.0), whereas strain CSW2.24.4(T) was able to grow at 25-45 °C (optimum 37 °C), with 2.6-5.1 M NaCl (optimum 3.4 M NaCl), with 0.01-0.7 M MgCl(2) (optimum 0.05 M MgCl(2)) and at pH 5.5-9.5 (optimum pH 7.0-7.5). Cells of the two isolates lysed in distilled water. The minimum NaCl concentrations that prevented cell lysis were 8 % (w/v) for strain TBN53(T) and 12 % (w/v) for strain CSW2.24.4(T). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate, with two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. Trace amounts of other unidentified lipids were also detected. On the basis of 16S rRNA gene sequence analysis, strains TBN53(T) and CSW2.24.4(T) showed 94.1 % similarity to each other and were closely related to Halobellus clavatus TNN18(T) (95.0 and 94.7 % similarity, respectively). Levels of rpoB' gene sequence similarity between strains TBN53(T) and CSW2.24.4(T), and between these strains and Halobellus clavatus TNN18(T) were 88.5, 88.5 and 88.1 %, respectively. The DNA G+C contents of strains TBN53(T) and CSW2.24.4(T) were 69.2 and 67.0 mol%, respectively. The level of DNA-DNA relatedness between strain TBN53(T) and strain CSW2.24.4(T) was 25 %, and these two strains showed low levels of DNA-DNA relatedness with Halobellus clavatus TNN18(T) (30 and 29 % relatedness, respectively). Based on these phenotypic, chemotaxonomic and phylogenetic properties, two novel species of the genus Halobellus are proposed to accommodate these two strains, Halobellus limi sp. nov. (type strain TBN53(T) = CGMCC 1.10331(T) = JCM 16811(T)) and Halobellus salinus sp. nov. (type strain CSW2.24.4(T) = DSM 18730(T) = CGMCC 1.10710(T) = JCM 14359(T)). PMID:22661071

  15. Draft Whole-Genome Sequence of Serratia sp. Strain TEL, Associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae) Isolated from a Grassland in South Africa

    PubMed Central

    Featherston, Jonathan; Gray, Vincent M.

    2015-01-01

    Here, we report on the draft genome sequence of Serratia sp. strain TEL, associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae, KM492926) isolated from a grassland in Suikerbosrand Nature Reserve near Johannesburg in South Africa. Serratia sp. strain TEL has a genome size of 5,000,541 bp with 4,647 genes and a G+C content of 59.1%. PMID:26159531

  16. Genetic Complexity of the Human Innate Host Defense Molecules, Surfactant Protein A1 (SP-A1) and SP-A2—Impact on Function

    PubMed Central

    Floros, Joanna; Wang, Guirong; Mikerov, Anatoly N.

    2010-01-01

    Innate immunity mechanisms play a critical role in the primary response to invading pathogenic microorganisms and other insulting agents. The innate lung immune system includes lung surfactant, a lipoprotein complex that carries out a function essential for life, that is, reduction of the surface tension at the air–liquid interphase of the alveolar space. By means of this function, pulmonary surfactant prevents lung collapse, therefore ensuring normal lung function and lung health. Pulmonary surfactant contains a number of host-defense molecules that are involved in the elimination of pathogens, viruses, particles, allergens, and other insults, as well as in the control of inflammation. This review is concerned with one of the surfactant proteins, the human (h) surfactant protein A (hSP-A), which, in addition to its role in surfactant-related functions, plays an important role in the modulation of lung host defense. The hSP-A locus has been identified with extensive complexity that may have an impact on its function, structure, and regulation. In humans, two genes—SP-A1 (SFTPA1) and SP-A2 (SFTPA2)—encode SP-A, with SP-A2 gene products being more biologically active than SP-A1 in most of the in vitro assays investigated. Although the two hSP-A genes share a high level of sequence similarity, differences in the structure and function between SP-A1 and SP-A2 have been observed in recent studies. In this review, we discuss the human SP-A complexity and how this may affect SP-A function. PMID:19392648

  17. 75 FR 42411 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Daily Contract; SP-15 Financial Day...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ...On October 6, 2009, the Commodity Futures Trading Commission (``CFTC'' or ``Commission'') published for comment in the Federal Register\\1\\ a notice of its intent to undertake a determination whether the SP-15\\2\\ Financial Day-Ahead LMP Peak Daily (``SDP'') contract; SP- 15 Financial Day-Ahead LMP Off-Peak Daily (``SQP'') contract; SP-15 Financial Swap Real Time LMP-Peak Daily (``SRP'')......

  18. Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules.

    PubMed

    Rashid, M Harun-or; Young, J Peter W; Everall, Isobel; Clercx, Pia; Willems, Anne; Santhosh Braun, Markus; Wink, Michael

    2015-09-01

    Rhizobial strains isolated from effective root nodules of field-grown lentil (Lens culinaris) from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (recA, atpD and glnII) and three nodulation genes (nodA, nodC and nodD), DNA fingerprinting and phenotypic characterization. Analysis of housekeeping gene sequences and DNA fingerprints indicated that the strains belonged to three novel clades in the genus Rhizobium. In present study, a representative strain from each clade was further characterized by determination of cellular fatty acid compositions, carbon substrate utilization patterns and DNA-DNA hybridization and average nucleotide identity (ANI) analyses from whole-genome sequences. DNA-DNA hybridization showed 50-62% relatedness to their closest relatives (the type strains of Rhizobium etli and Rhizobium phaseoli) and 50-60% relatedness to each other. These results were further supported by ANI values, based on genome sequencing, which were 87-92% with their close relatives and 88-89% with each other. On the basis of these results, three novel species, Rhizobium lentis sp. nov. (type strain BLR27(T) = LMG 28441(T) = DSM 29286(T)), Rhizobium bangladeshense sp. nov. (type strain BLR175(T) = LMG 28442(T) = DSM 29287(T)) and Rhizobium binae sp. nov. (type strain BLR195(T) = LMG 28443(T) = DSM 29288(T)), are proposed. These species share common nodulation genes (nodA, nodC and nodD) that are similar to those of the symbiovar viciae. PMID:26060217

  19. Gluconacetobacter tumulisoli sp. nov., Gluconacetobacter takamatsuzukensis sp. nov. and Gluconacetobacter aggeris sp. nov., isolated from Takamatsuzuka Tumulus samples before and during the dismantling work in 2007.

    PubMed

    Nishijima, Miyuki; Tazato, Nozomi; Handa, Yutaka; Tomita, Junko; Kigawa, Rika; Sano, Chie; Sugiyama, Junta

    2013-11-01

    Ten strains of Gram-stain-negative, rod-shaped, non-spore-forming bacteria were isolated from the burial mound soil collected before the dismantling and samples collected during the dismantling work on the Takamatsuzuka Tumulus in Asuka village, Nara Prefecture, Japan in 2007. On the basis of the 16S rRNA gene sequence analysis of the isolates, they were accommodated in the genus Gluconacetobacter (class Alphaproteobacteria) and can be separated into four groups within the cluster containing the genus Gluconacetobacter. One of the groups demonstrated a phylogenetic position identical to that of Gluconacetobacter asukensis, which was isolated from small holes on plaster walls of the stone chamber interior of Kitora Tumulus in Asuka village, Nara Prefecture, Japan. The remaining three groups consisted of novel lineages within the genus Gluconacetobacter. A total of four isolates were selected from each group and carefully identified using a polyphasic approach. The isolates were characterized on the basis of their possessing Q-10 as the major ubiquinone system and C18?:?1?7c (58.5-65.2?%) as the predominant fatty acid. A DNA-DNA hybridization test was used to determine that the three lineages represented novel species, for which the names Gluconacetobacter tumulisoli sp. nov., Gluconacetobacter takamatsuzukensis sp. nov. and Gluconacetobacter aggeris sp. nov. are proposed. The type strains are T611xx-1-4a(T) (?=?JCM 19097(T)?=?NCIMB 14861(T)), T61213-20-1a(T) (?=?JCM 19094(T)?=?NCIMB 14859(T)) and T6203-4-1a(T) (?=?JCM 19092(T)?=?NCIMB 14860(T)), respectively. PMID:23793855

  20. Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica.

    PubMed

    Hirsch, Peter; Gallikowski, Claudia A; Siebert, Jörg; Peissl, Klaus; Kroppenstedt, Reiner; Schumann, Peter; Stackebrandt, Erko; Anderson, Robert

    2004-11-01

    Six Gram-positive, non-motile, UV- and draught-tolerant bacteria were isolated from antarctic soil and rock samples. The pink to orange cocci grew well on oligotrophic medium PYGV (pH 7.5) at 9-18 degrees C. They tolerated 0-10% NaCl, were aerobic to facultatively anaerobic and contained ornithine in their cell wall (type A3beta, Orn-Gly2). The lipid profiles of four strains were found to be typical for those of D. radiodurans. Major fatty acids were 16:1cis9, 15:1cis9, 17:1cis9 and i17:1cis9, the respiratory quinone of three strains was MK-8. Comparative 16S rDNA gene sequencing revealed phylogenetic relationships to the Deinococcus clade, especially to D. radiopugnans. The levels of 16S rRNA gene sequence similarity and DNA-DNA hybridisation data showed the six isolates represented new taxa. Phenotypic properties supported the description of three new species which were different from the eight known Deinococcus species and particularly from D. radiopugnans. Soil isolate AA-692T (DSM 12807T) is the type strain of Deinococcus frigens sp. nov., with AA-752 (DSM 15993) and AA-829 (DSM 15994) as additional strains from soil. The endolithic isolate AA-1444T, Deinococcus saxicola sp. nov., (DSM 15974T) came from antarctic sandstone, and Deinococcus marmoris sp. nov. (isolate AA-63T [DSM 12784T]) as well as AA-69 (DSM 15951) were isolated from antarctic marble. PMID:15612620

  1. Super-parameterization in GRAPES: The construction of SP-GRAPES and associated preliminary results

    NASA Astrophysics Data System (ADS)

    Zhu, Feng; Xu, Guoqiang; Zheng, Xiaohui; Wang, Yuhong

    2015-04-01

    Super-parameterization (SP) aims to explicitly represent deep convection within a coarse resolution global model by embedding a cloud resolving model (CRM) in each column of the mother model. For the first time, we implemented the SP in a mesoscale regional weather model, the Global/Regional Assimilation and PrEdiction System (GRAPES). The constructed SP-GRAPES uses a two-dimensional (2D) CRM in each grid column. A control and two SP simulations are conducted for the Beijing "7.21" heavy rainfall event to evaluate improvements in GRAPES using SP. The SP-run-I is a basic SP run delivering microphysics feedback only, whereas the SP-run-II delivers both microphysical and cloud fraction feedbacks. A comparison of the runs indicates that the SP-run-I has a slightly positive impact on the precipitation forecast than the control run. However, the inclusion of cloud fraction feedback leads to an evident overall improvement, particularly in terms of cloud fraction and 24-h cumulative precipitation. Although this is only a preliminary study using SP-GRAPES, we believe that it will provide considerable guidance for follow-up studies using SP in China.

  2. A hydrogen-evolving enzyme is present in Frankia sp. R43.

    PubMed

    Mohapatra, Anasuya; Leul, Melakeselam; Mattsson, Ulrika; Sellstedt, Anita

    2004-07-15

    The ability to evolve hydrogen using methyl viologen as an electron donor was assayed in the nitrogen-fixing actinomycetes Frankia sp. R43 and Frankia sp. KB5. To further examine the nature of hydrogen-evolving enzymes that may be present in these organisms immunological studies were performed. Under anaerobic conditions (both nitrogen-limiting and nitrogen-containing) Frankia sp. R43 but not Frankia sp. KB5 evolved hydrogen,which was not linked to NAD-reducing activity. Immunological analysis of total protein from Frankia sp. R43 and Frankia sp. KB5 using an antiserum raised against Ralstonia eutropha HoxF, recognized an antigen in Frankia sp. R43 but not in Frankia sp. KB5. Immunogold labeling using antibodies raised against the R. eutropha HoxH recognized sites in both hyphae and vesicles of Frankia sp. R43, but not in Frankia sp. KB5. Based on these physiological and immunological findings, we conclude that Frankia sp. R43 has a hydrogen-evolving hydrogenase. PMID:15251202

  3. Role of zinc finger structure in nuclear localization of transcription factor Sp1

    SciTech Connect

    Ito, Tatsuo; Azumano, Makiko; Uwatoko, Chisana; Itoh, Kohji Kuwahara, Jun

    2009-02-27

    Transcription factor Sp1 is localized in the nucleus and regulates gene expression. Our previous study demonstrated that the carboxyl terminal region of Sp1 containing 3-zinc finger region as DNA binding domain can also serve as nuclear localization signal (NLS). However, the nuclear transport mechanism of Sp1 has not been well understood. In this study, we performed a gene expression study on mutant Sp1 genes causing a set of amino acid substitutions in zinc finger domains to elucidate nuclear import activity. Nuclear localization of the GFP-fused mutant Sp1 proteins bearing concomitant substitutions in the first and third zinc fingers was highly inhibited. These mutant Sp1 proteins had also lost the binding ability as to the GC box sequence. The results suggest that the overall tertiary structure formed by the three zinc fingers is essential for nuclear localization of Sp1 as well as dispersed basic amino acids within the zinc fingers region.

  4. Nitrate levels modulate the abundance of Paracoccus sp. in a biofilm community.

    PubMed

    Singh, Shantanu; Nerurkar, Anuradha S; Srinandan, C S

    2015-06-01

    Conditions required to enhance a particular species efficient in degradative capabilities is very useful in wastewater treatment processes. Paracoccus sp. is known to efficiently reduce nitrogen oxides (NOx) due to the branched denitrification pathway. Individual-based simulations showed that the relative fitness of Paracoccus sp. to Pseudomonas sp. increased significantly with nitrate levels above 5 mM. Spatial structure of the biofilm showed substantially less nitrite levels in the areas of Paracoccus sp. dominance. The simulation was validated in a laboratory reactor harboring biofilm community by fluorescent in situ hybridization, which showed that increasing nitrate levels enhanced the abundance of Paracoccus sp. Different levels of NOx did not display any significant effect on biofilm formation of Paracoccus sp., unlike several other bacteria. This study shows that the attribute of Paracoccus sp. to tolerate and efficiently reduce NOx is conferring a fitness payoff to the organism at high concentrations of nitrate in a multispecies biofilm community. PMID:25838197

  5. Induced parasexual processes in Claviceps sp. strain SD58.

    PubMed Central

    Brauer, K L; Robbers, J E

    1987-01-01

    A homokaryotic, clavine alkaloid-producing strain of ergot, Claviceps sp. strain SD 58, was used in an attempt to demonstrate parasexuality. Genetically marked auxotrophic strains were produced by mutation with N-methyl-N'-nitro-N-nitrosoguanidine. Protoplast fusion of pairs of unlike doubly auxotrophic strains and isolation of stable prototrophic fusion products were carried out. By growth of the fusion products on complete medium, selective pressure for prototrophy was removed and auxotrophic segregants were allowed to form. Analysis of these and recovery of segregants with nonleaky, non-parent-type combinations of auxotrophic characteristics has provided strong evidence that a parasexual cycle can function in Claviceps sp. strain SD 58. Preliminary work suggests that the genetics of ergot might be studied by mitotic analysis and that protoplast fusion and selection procedures might be useful for the enhancement of favorable characteristics in Claviceps strains. PMID:3827250

  6. PWC-11 fuel pin development for SP-100

    NASA Astrophysics Data System (ADS)

    Sayre, Edwin D.; Butler, Robert E.; Kangilaski, Mike

    1992-01-01

    GE has developed a high temperature fuel pin for the SP-100 Space Reactor. The Nb-1% Zr alloy PWC-11 was selected over the conventional Nb-1% Ar alloy for the fuel pin cladding because the higher creep strength enables a significant reduction in system mass. PWC-11 has 0.1% carbon to provide the higher creep and tensile strength at the operating temperature. PWC-11 was developed by Pratt and Whitney in the 1960's and it has also been investigated by the Russians. Six small 35 pound ingots were purchased from Teledyne Wah Chang, five by Nasa Lewis Research Center, and one by GE for the SP-100. Since no work had been done during the last two decades, processes had to be developed based on the work done earlier. The tube making process experience at GE is discussed.

  7. NSS5/SP-STM2 Joint International Conference

    SciTech Connect

    Saw-Wai Hla

    2009-05-03

    The NSS5/SP-STM2 conference was held in Athens, Ohio July 15-19, 2008. The conference brought together a prestigious group of scientists from all over the globe to focus for 3 ½ days on a variety of nanoscience topics, particularly on nanoscale spectroscopy and spin-polarized scanning tunneling microscopy. The conference was attended by many young scientists as well as senior scientists. Attendees to the conference were drawn from more than 10 countries and included 28 invited speakers, who are the leading scientists in their respective research areas. Included among the invited speakers were 4 plenary speakers - eminent scientists in their fields. The conference was divided into two parallel sessions – the NSS5 session and the SP-STM2 session.

  8. Die Aufnahme partikulärer Nahrung bei Reniera sp. (Porifera)

    NASA Astrophysics Data System (ADS)

    Langenbruch, Paul-Friedrich

    1985-09-01

    The choanocyte chambers of the marine sponge Reniera sp. protrude with their curved outer surface free into the incurrent canals. The water is sucked into the chambers by cavities between the choanocytes. Particles up to 1 µm in diameter may enter the chambers with the water current. These particles are trapped on the outer surface of the choanocyte collars and are ingested by the choanocytes and processes of the pinacocyte epithelium of the incurrent canal system, which project into the chambers. Bigger particles are retained in the incurrent canals mainly on the outer surface of the choanocyte chambers. They are ingested by pinacocytes of the canal wall and transported to cells of the mesenchyme. The present investigation shows the great importance of the pinacocyte epithelium of the incurrent canal system for suspension feeding in Reniera sp.

  9. Helicobacter sp. Flexispira Bacteremia in an Immunocompetent Young Adult

    PubMed Central

    Iten, Anne; Graf, Susanne; Egger, Martin; Täuber, Martin; Graf, Joerg

    2001-01-01

    A young immunocompetent patient was admitted for a febrile illness with malaise, arthralgias, painful leg swelling, and polyserositis. Shortly prior to becoming ill, the patient had traveled to the Northern African desert. The symptoms disappeared during treatment with antibiotics (doxycycline and ceftriaxone) but recurred twice after stopping therapy. A motile gram-negative fusiform rod was isolated from a blood culture taken on the first admission. Analysis of the 16S rRNA gene of the blood culture isolate revealed close similarity with Helicobacter sp. flexispira taxon 8 (99.9% identity), a species that was previously reported as “Flexispira rappini.” This is the first reported case of a recurrent Helicobacter sp. flexispira bacteremia in an adult, immunocompetent patient. PMID:11325980

  10. Effect of temperature on ammonium removal in Scenedesmus sp.

    PubMed

    Ruiz-Martínez, A; Serralta, J; Seco, A; Ferrer, J

    2015-09-01

    The effect of temperature on microalgal ammonium uptake was investigated by carrying out four batch experiments in which a mixed culture of microalgae, composed mainly of Scenedesmus sp., was cultivated under different temperatures within the usual temperature working range in Mediterranean climate (15-34 °C). Ammonium removal rates increased with temperature up to 26 °C and stabilized thereafter. Ratkowsky and Cardinal temperatures models successfully reproduced the experimental data. Optimum (31.3 °C), minimum (8.8 °C) and maximum (46.1 °C) temperatures for ammonium removal by Scenedesmus sp. under the studied conditions were obtained as model parameters. These temperature-related parameters constitute very useful information for designing and operating wastewater treatment systems using these microalgae. PMID:26027902

  11. Genome sequence and description of Corynebacterium ihumii sp. nov.

    PubMed Central

    Padmanabhan, Roshan; Dubourg, Grégory; Lagier, Jean-Christophe; Couderc, Carine; Michelle, Caroline; Raoult, Didier; Fournier, Pierre-Edouard

    2014-01-01

    Corynebacterium ihumii strain GD7T sp. nov. is proposed as the type strain of a new species, which belongs to the family Corynebacteriaceae of the class Actinobacteria. This strain was isolated from the fecal flora of a 62 year-old male patient, as a part of the culturomics study. Corynebacterium ihumii is a Gram positive, facultativly anaerobic, nonsporulating bacillus. Here, we describe the features of this organism, together with the high quality draft genome sequence, annotation and the comparison with other member of the genus Corynebacteria. C. ihumii genome is 2,232,265 bp long (one chromosome but no plasmid) containing 2,125 protein-coding and 53 RNA genes, including 4 rRNA genes. The whole-genome shotgun sequence of Corynebacterium ihumii strain GD7T sp. nov has been deposited in EMBL under accession number GCA_000403725. PMID:25197488

  12. Complete genome sequence of Paenibacillus sp. strain JDR-2

    SciTech Connect

    Chow, Virginia; Nong, Guang; St. John, Franz J.; Dickstein, Ellen; Chertkov, Olga; Bruce, David; Detter, J. Chris; Brettin, Thomas S; Han, James; Woyke, Tanja; Pitluck, Sam; Nolan, Matt; Pati, Amrita; Martin, Joel; Copeland, A; Land, Miriam L; Goodwin, Lynne A.; Jones, Jeffrey B.; Ingram, Lonnie O.; Shanmugam, Keelnathan T.; Preston, James F.

    2012-01-01

    Paenibacillus sp. strain JDR-2, an aggressively xylanolytic bacterium isolated from sweetgum (Liquidambar styraciflua) wood, is able to efficiently depolymerize, assimilate and metabolize 4-O-methylglucuronoxylan, the predominant structural component of hardwood hemicelluloses. A basis for this capability was first supported by the identification of genes and characterization of encoded enzymes and has been further defined by the sequencing and annotation of the complete genome, which we describe. In addition to genes implicated in the utilization of -1,4-xylan, genes have also been identified for the utilization of other hemicellulosic polysaccharides. The genome of Paenibacillus sp. JDR-2 contains 7,184,930 bp in a single replicon with 6,288 protein-coding and 122 RNA genes. Uniquely prominent are 874 genes encoding proteins involved in carbohydrate transport and metabolism. The prevalence and organization of these genes support a metabolic potential for bioprocessing of hemicellulose fractions derived from lignocellulosic resources.

  13. Tongue adhesion in the horned frog Ceratophrys sp.

    NASA Astrophysics Data System (ADS)

    Kleinteich, Thomas; Gorb, Stanislav N.

    2014-06-01

    Frogs are well-known to capture elusive prey with their protrusible and adhesive tongues. However, the adhesive performance of frog tongues and the mechanism of the contact formation with the prey item remain unknown. Here we measured for the first time adhesive forces and tongue contact areas in living individuals of a horned frog (Ceratophrys sp.) against glass. We found that Ceratophrys sp. generates adhesive forces well beyond its own body weight. Surprisingly, we found that the tongues adhered stronger in feeding trials in which the coverage of the tongue contact area with mucus was relatively low. Thus, besides the presence of mucus, other features of the frog tongue (surface profile, material properties) are important to generate sufficient adhesive forces. Overall, the experimental data shows that frog tongues can be best compared to pressure sensitive adhesives (PSAs) that are of common technical use as adhesive tapes or labels.

  14. Novel cyclohexane monooxygenase from Acidovorax sp. CHX100.

    PubMed

    Salamanca, Diego; Karande, Rohan; Schmid, Andreas; Dobslaw, Daniel

    2015-08-01

    Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5-C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5-C8) to their respective cyclic alcohols. PMID:25935342

  15. Siphateles (Gila) sp. and Catostomus sp. from the Pleistocene OIS-6 Lake Gale, Panamint Valley, Owens River system, California

    NASA Astrophysics Data System (ADS)

    Jayko, A. S.; Forester, R. M.; Smith, G. R.

    2014-12-01

    Panamint Valley lies within the Owens River system which linked southeastern Sierra Nevada basins between Mono Lake and Death Valley during glacial-pluvial times. Previous work indicates that late Pleistocene glacial-pluvial Lake Gale, Panamint Valley was an open system during OIS-6, a closed ground water supported shallow lake during OIS-4, and the terminal lake basin for the Owens River system during OIS-2. We here report the first occurrence of fossil fish from the Plio-Pleistocene Panamint basin. Fish remains are present in late Pleistocene OIS-6 nearshore deposits associated with a highstand that was spillway limited at Wingate Wash. The deposits contain small minnow-sized remains from both Siphateles or Gila sp. (chubs) and Catostomus sp. (suckers) from at least four locations widely dispersed in the basin. Siphateles or Gila sp. and Catostomus are indigenous to the Pleistocene and modern Owens River system, in particular to the historic Owens Lake area. Cyprinodon (pupfish) and Rhinichthys (dace) are known from the modern Amargosa River and from Plio-Pleistocene deposits in Death Valley to the east. The late Pleistocene OIS-6 to OIS-2 lacustrine and paleohydrologic record in Panamint basin is interpreted from ostracod assemblages, relative abundance of Artemia sp. pellets, shallow water indicators including tufa fragments, ruppia sp. fragments and the relative abundance of charophyte gyrogonites obtained from archived core, as well as faunal assemblages from paleoshoreline and nearshore deposits. The OIS-4 groundwater supported shallow saline lake had sufficiently low ratios of alkalinity to calcium (alk/Ca) to support the occurrence of exotic Elphidium sp. (?) foraminfera which are not observed in either OIS-2 or OIS-6 lacustrine deposits. The arrival of Owens River surface water into Panamint Basin during OIS-2 is recorded by the first appearance of the ostracod Limnocythere sappaensis at ~27 m depth in an ~100 m archived core (Smith and Pratt, 1957) which extends between OIS-5 and post OIS-2 based on based on proxy correlation with the marine oxygen isotope record.

  16. The transputer based GA. SP data acquisition system

    SciTech Connect

    Colombo, D.; Avano, B.; DePoli, M.; Maron, G. ); Negro, A.; Parlati, G. )

    1992-04-01

    In this paper, the new data acquisition for the GA.SP detector is presented. It is a distributed system based on a network of 40 T800 and T222 transputers linked to a VME system used for histogram storage. A 100 MBit/s FDDI ring connects the system to UNIX workstations used for the experiment control, histogram display and second level data analysis.

  17. New oxidized zoanthamines from a Canary Islands Zoanthus sp.

    PubMed

    Cen-Pacheco, Francisco; Martín, Manuel Norte; Fernández, José Javier; Hernández Daranas, Antonio

    2014-10-01

    Three new norzoanthamine-type alkaloids, named 2-hydroxy-11-ketonorzoan thamide B (1), norzoanthamide B (2) and 15-hydroxynorzoanthamine (3), were isolated from Zoanthus sp. specimens collected at the Canary Islands. Their structures were determined by interpretation of NMR and HR-ESIMS data. Relative configurations of their chiral centers were proposed on the basis of ROESY spectra and by comparison of their spectroscopic data with those of the well-known compound, norzoanthamine. PMID:25317536

  18. Ultrastructure of Bonamia sp. in Ostrea chilensis in Chile.

    PubMed

    Lohrmann, K B; Hine, P M; Campalans, M

    2009-07-23

    Oyster Ostrea chilensis samples were collected from Quihua Island, Chile, in December 2003 and February 2005, and examined in May 2004, and March, April and July 2005, for an ultrastructural comparison of the Chilean Bonamia sp. with other Bonamia spp. Only uni-nucleate stages were encountered, except in the July sample. The Chilean parasite differs from B. perspora in the apparent lack of a plasmodial stage and of sporulation. It resembles B. ostreae in size, the low number of mitochondrial profiles, and the prevalence and mean number of lipid droplets. It differs from B. ostreae in the greater prevalence of nuclear membrane-bound Golgi (NM-BG), associated haplosporogenesis, and smaller size of haplosporosomes. The Chilean Bonamia sp. resembles B. exitiosa in the number of haplosporosomes, prevalence of lipid droplets, anastomosing endoplasmic reticulum and NM-BG, presence of circles of smooth endoplasmic reticulum (sER), confronting cisternae (CC), and cylindrical CC (CCC). It also appears to have a similar developmental cycle to B. exitiosa with larger forms occurring in winter (August). The circles of sER, CC, and CCC have only been reported from B. exitiosa, and it appears that Chilean Bonamia sp. and B. exitiosa are more closely related than they are to B. perspora or B. ostreae. Similarities in ultrastructure and developmental stages between New Zealand and Chilean parasites suggest that the 2 species are related, and that the Chilean Bonamia sp. is either B. exitiosa, a sub-species of B. exitiosa, or a separate species closely related to B. exitiosa. PMID:19750808

  19. Cytotoxic Sesterterpenoids Isolated from the Marine Sponge Scalarispongia sp.

    PubMed Central

    Lee, Yeon-Ju; Lee, Jeong-Woo; Lee, Dong-Geun; Lee, Hyi-Seung; Kang, Jong Soon; Yun, Jieun

    2014-01-01

    Eight scalarane sesterterpenoids, including four new compounds, were isolated from the marine sponge Scalarispongia sp. The structures of the new compounds were elucidated by 2D-NMR and HRMS analyses. All of the isolated compounds, with the exception of 16-O-deacetyl-12,16-epi-scalarolbutanolide, showed significant in vitro cytotoxicity (GI50 values down to 5.2 ?M) against six human cancer cell lines. PMID:25375188

  20. Five New Diterpenoids from an Okinawan Soft Coral, Cespitularia sp.

    PubMed Central

    Roy, Prodip K.; Maarisit, Wilmar; Roy, Michael C.; Taira, Junsei; Ueda, Katsuhiro

    2012-01-01

    Five new diterpenoids 1–5 were isolated from an Okinawan soft coral, Cespitularia sp., together with the known diterpenoid, alcyonolide (6). New diterpenoid structures were elucidated by spectroscopic methods and by comparison of their NMR data with those of related compounds. Alcyonolide (6) was cytotoxic against HCT 116 cells (IC50 5.85 ?M), while these new diterpenoids 1–5 were much less active (IC50 28.2–91.4 ?M). PMID:23201595

  1. Genome sequencing and annotation of Morganella sp. SA36

    PubMed Central

    Selim, Samy; Hassan, Sherif; Hagagy, Nashwa

    2015-01-01

    We report draft genome sequence of Morganella sp. Strain SA36, isolated from water spring in Aljouf region, Saudi Arabia. The draft genome size is 2,564,439 bp with a G + C content of 51.1% and contains 6 rRNA sequence (single copies of 5S, 16S & 23S rRNA). The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession no. LDNQ00000000.

  2. Complete mitochondrial genome of Platevindex sp. (Gastropoda: Pulmonata: Systellommatophora: Onchidiidae).

    PubMed

    Liu, Chen; Shen, He Ding; Zhou, Na

    2016-03-01

    The complete mitochondrial genome sequence of Platevindex sp. is firstly described in the article. The mitogenome (13,908?bp) contains 22 tRNA genes, 2 ribosomal RNA genes and 13 protein-coding genes, and 1 putative control region (CR). CR is not well characterized due to lack of discrete conserved sequence blocks. This characteristic is similar with CRs of other invertebrate mitochondrial genomes. The characteristic is the typical bivalvia mitochondrial gene composition. PMID:24938092

  3. ARSENIC TRIOXIDE DOWNREGULATES SPECIFICITY PROTEIN (Sp) TRANSCRIPTION FACTORS AND INHIBITS BLADDER CANCER CELL AND TUMOR GROWTH

    PubMed Central

    Jutooru, Indira; Chadalapaka, Gayathri; Sreevalsan, Sandeep; Lei, Ping; Barhoumi, Rola; Burghardt, Robert; Safe, Stephen

    2010-01-01

    Arsenic trioxide exhibits antiproliferative, antiangiogenic and proapoptotic activity in cancer cells, and many genes associated with these responses are regulated by specificity protein (Sp) transcription factors. Treatment of cancer cells derived from urologic (bladder and prostate) and gastrointestinal (pancreas and colon) tumors with arsenic trioxide demonstrated that these cells exhibited differential responsiveness to the antiproliferative effects of this agent and this paralleled their differential repression of Sp1, Sp3 and Sp4 proteins in the same cell lines. Using arsenic trioxide responsive KU7 and non-responsive 253JB-V bladder cancer cells as models, we show that in KU7 cells, ? 5 ?M arsenic trioxide decreased Sp1, Sp3 and Sp4 and several Sp-dependent genes and responses including cyclin D1, epidermal growth factor receptor, bcl-2, survivin and vascular endothelial growth factor, whereas at concentrations up to 15 ?M, minimal effects were observed in 253JB-V cells. Arsenic trioxide also inhibited tumor growth in athymic mice bearing KU7 cells as xenografts, and expression of Sp1, Sp3 and Sp4 was significantly decreased. Inhibitors of oxidative stress such as glutathione or dithiothreitol protected KU7 cells from arsenic trioxide-induced antiproliferative activity and Sp repression, whereas glutathione depletion sensitized 253JB-V cells to arsenic trioxide. Mechanistic studies suggested that arsenic trioxide-dependent downregulation of Sp and Sp-dependent genes was due to decreased mitochondrial membrane potential and induction of reactive oxygen species, and the role of peroxides in mediating these responses was confirmed using hydrogen peroxide. PMID:20435036

  4. Users guide to the Argonne SP scheduling system

    SciTech Connect

    Lifka, D.A. |; Henderson, M.W.; Rayl, K.

    1995-05-01

    During the past five years scientists discovered that modern UNIX workstations connected with ethernet and fiber networks could provide enough computational performance to compete with the supercomputers of the day. As this concept became increasingly popular, the need for distributed queuing and scheduling systems became apparent. Today, supercomputers, such as Argonne National Laboratory`s IBM SP system, can provide more CPU and networking speed than can be obtained from these networks of workstations. These modern supercomputers look like clusters of workstations, however, so developers felt that the scheduling systems that were previously used on clusters of workstations should still apply. After trying to apply some of these scheduling systems to Argonne`s SP environment, it became obvious that these two computer environments have very different scheduling needs. Recognizing this need and realizing that no one has addressed it, we developed a new scheduling system. The approach taken in creating this system was unique in that user input and interaction were encouraged throughout the development process. Thus, a scheduler was built that actually worked the way the users wanted it to work. This document serves a dual purpose. It is both a user`s guide and an administrator`s guide for the ANL SP scheduling system. Look for revisions to this guide that will be appearing.

  5. Purification and characterization of chitinase from Paenibacillus sp. D1.

    PubMed

    Singh, Anil Kumar; Chhatpar, Hari S

    2011-05-01

    A 56.56-kDa extracellular chitinase from Paenibacillus sp. D1 was purified to 52.3-fold by ion exchange chromatography using SP Sepharose. Maximum enzyme activity was recorded at pH 5.0 and 50 °C. MALDI-LC-MS/MS analysis identified the purified enzyme as chitinase with 60% similarity to chitinase Chi55 of Paenibacillus ehimensis. The activation energy (E (a)) for chitin hydrolysis and temperature quotient (Q (10)) at optimum temperature was found to be 19.14 kJ/mol and 1.25, respectively. Determination of kinetic constants k (m), V (max), k (cat), and k (cat)/k (m) and thermodynamic parameters ?H*, ?S*, ?G*, ?G*(E-S), and ?G*(E-T) revealed high affinity of the enzyme for chitin. The enzyme exhibited higher stability in presence of commonly used protectant fungicides Captan, Carbendazim, and Mancozeb compared to control as reflected from the t (1/2) values suggesting its applicability in integrated pest management for control of soil-borne fungal phytopathogens. The order of stability of chitinase in presence of fungicides at 80 °C as revealed from t (1/2) values and thermodynamic parameters E (a(d)) (activation energy for irreversible deactivation), ?H*, ?G*, and ?S* was: Captan > Carbendazim > Mancozeb > control. The present study is the first report on thermodynamic and kinetic characterization of chitinase from Paenibacillus sp. D1. PMID:21049291

  6. Network Analysis of Plasmidomes: The Azospirillum brasilense Sp245 Case

    PubMed Central

    Fondi, Marco

    2014-01-01

    Azospirillum brasilense is a nitrogen-fixing bacterium living in association with plant roots. The genome of the strain Sp245, isolated in Brazil from wheat roots, consists of one chromosome and six plasmids. In this work, the A. brasilense Sp245 plasmids were analyzed in order to shed some light on the evolutionary pathways they followed over time. To this purpose, a similarity network approach was applied in order to identify the evolutionary relationships among all the A. brasilense plasmids encoded proteins; in this context a computational pipeline specifically devoted to the analysis and the visualization of the network-like evolutionary relationships among different plasmids molecules was developed. This information was supplemented with a detailed (in silico) functional characterization of both the connected (i.e., sharing homology with other sequences in the dataset) and the unconnected (i.e., not sharing homology) components of the network. Furthermore, the most likely source organism for each of the genes encoded by A. brasilense plasmids was checked, allowing the identification of possible trends of gene loss/gain in this microorganism. Data obtained provided a detailed description of the evolutionary landscape of the plasmids of A. brasilense Sp245, suggesting some of the molecular mechanisms responsible for the present-day structure of these molecules. PMID:25610702

  7. SP-100 reactor with Brayton conversion for lunar surface applications

    SciTech Connect

    Mason, L.S.; Rodriguez, C.D.; Mckissock, B.I.; Hanlon, J.C.; Mansfield, B.C.

    1992-01-01

    Examined here is the potential for integrating Brayton-cycle power conversion with the SP-100 reactor for lunar surface power system applications. Two designs were characterized and modeled. The first design integrates a 100-kWe SP-100 Brayton power system with a lunar lander. This system is intended to meet early lunar mission power needs while minimizing on-site installation requirements. Man-rated radiation protection is provided by an integral multilayer, cylindrical lithium hydride/tungsten (LiH/W) shield encircling the reactor vessel. Design emphasis is on ease of deployment, safety, and reliability, while utilizing relatively near-term technology. The second design combines Brayton conversion with the SP-100 reactor in a erectable 550-kWe powerplant concept intended to satisfy later-phase lunar base power requirements. This system capitalizes on experience gained from operating the initial 100-kWe module and incorporates some technology improvements. For this system, the reactor is emplaced in a lunar regolith excavation to provide man-rated shielding, and the Brayton engines and radiators are mounted on the lunar surface and extend radially from the central reactor. Design emphasis is on performance, safety, long life, and operational flexibility.

  8. Biodegradation of Para Amino Acetanilide by Halomonas sp. TBZ3

    PubMed Central

    Hajizadeh, Nader; Sefidi Heris, Youssof; Zununi Vahed, Sepideh; Vallipour, Javad; Hejazi, Mohammad Amin; Golabi, Sayyed Mahdi; Asadpour-Zeynali, Karim; Hejazi, Mohammad Saeid

    2015-01-01

    Background: Aromatic compounds are known as a group of highly persistent environmental pollutants. Halomonas sp. TBZ3 was isolated from the highly salty Urmia Lake of Iran. In this study, characterization of a new Halomonas isolate called Halomonas sp. TBZ3 and its employment for biodegradation of para-amino acetanilide (PAA), as an aromatic environmental pollutant, is described. Objectives: This study aimed to characterize the TBZ3 isolate and to elucidate its ability as a biodegradative agent that decomposes PAA. Materials and Methods: Primarily, DNA-DNA hybridization between TBZ3, Halomonas denitrificans DSM18045T and Halomonas saccharevitans LMG 23976T was carried out. Para-amino acetanilide biodegradation was assessed using spectrophotometry and confirmed by gas chromatography-mass spectroscopy (GC-MS). Parameters effective on biodegradation of PAA were optimized by the Response Surface Methodology (RSM). Results: The DNA-DNA hybridization experiments between isolate TBZ3, H. denitrificans and H. saccharevitans revealed relatedness levels of 57% and 65%, respectively. According to GC-MS results, TBZ3 degrades PAA to benzene, hexyl butanoate, 3-methyl-1-heptanol and hexyl hexanoate. Temperature 32.92°C, pH 6.76, and salinity 14% are the optimum conditions for biodegradation with a confidence level of 95% (at level ? = 0.05). Conclusions: According to our results, Halomonas sp. TBZ3 could be considered as a biological agent for bioremediation of PAA and possibly other similar aromatic compounds. PMID:26495103

  9. Hasanuddinia maxomyos n. gen., n. sp. and Heligmonoides musseri n. sp. (Nematoda: Heligmonellidae) collected from endemic murines of Sulawesi, Indonesia.

    PubMed

    Hasegawa, H; Syafruddin

    1994-10-01

    Two new nematodes belonging to the subfamily Nippostrongylinae (Nematoda: Trichostrongyloidea: Heligmonellidae) are described based on specimens from endemic murines of Sulawesi, Indonesia: Hasanuddinia maxomyos n. gen., n. sp. from Maxomys musschenbroekii and Eropeplus canus, and Heligmonoides musseri n. sp. from M. musschenbroekii, E. canus, and Margaretamys elegans. Hasanuddinia is closest to Rattusstrongylus of Malaysian rats in lacking a carene and in having a lateromedian gradient in the size of the synlophe ridges in the ventral side of the midbody but is distinguished in that the dorsal ray is divided in the distal half. Heligmonoides musseri most resembles H. bulbosus of Maxomys whiteheadi of Malaysia in having a carene supported by slender ridges of which the proximal portion is not thickened but is distinguished in having longer externodorsal rays and longer spicules. The ancestors of the present Sulawesi nematodes seem to have originated on the southeast Asian continent, were introduced to Sulawesi with the dispersal of some murines, and subsequently speciated. PMID:7931913

  10. Bensingtonia rectispora sp. nov. and Bensingtonia bomiensis sp. nov., ballistoconidium-forming yeast species from Tibetan plant leaves.

    PubMed

    Wang, Qi-Ming; Boekhout, Teun; Bai, Feng-Yan

    2012-08-01

    Five yeast strains isolated from plant leaves collected in south-east Tibet formed cream to brownish colonies and produced asymmetrical ballistoconidia and CoQ-9 as the major ubiquinone. Sequence analysis of the 26S rRNA D1/D2 domain and the internal transcribed spacer region indicated that these strains represented two novel species of the genus Bensingtonia. The names Bensingtonia rectispora sp. nov. (type strain XZ 4C5(T)?=?CGMCC 2.02635(T)?=?CBS 10710(T)) and Bensingtonia bomiensis sp. nov. (type strain XZ 33D1(T)?=?CGMCC 2.02670(T)?=?CBS 10713(T)) are proposed for the two novel species, which are phylogenetically closely related to Bensingtonia naganoensis, Bensingtonia pseudonaganoensis and the type species of the genus, Bensingtonia ciliata. PMID:22081720

  11. Sarcocystis dubeyella n. sp. and Sarcocystis phacochoeri n. sp. (Protozoa: Sarcocystidae) from the warthog (Phacochoerus aethiopicus) in South Africa.

    PubMed

    Stolte, M; Odening, K; Quandt, S; Bengis, R G; Bockhardt, I

    1998-01-01

    Sarcocystis dubeyella n. sp. and S. phacochoeri n. sp. from muscle fibers of the skeletal musculature of two warthogs in South Africa are described by light and and electron microscopy. Sarcocystis dubeyella sarcocysts are macroscopic (up to 12 mm long and 1 mm wide), with a parasite-induced encapsulation of the host muscle fiber in which the plasma membrane of the latter remained unaltered. The sarcocyst wall is characterized by evenly arranged, irregularly semicircular or rectangular villar protrusions (5.0 x 2.8-11.0 microns) with indented margins and no specific content. Sarcocystis phacochoeri formed filiform microcysts (up to 4 mm long and 0.13 mm wide). Its cyst wall is provided with tightly packed, molarlike villar protrusions (1.6-3.3 x 1.7-3.3 microns), with smooth margins, hollow on one side, and with longitudinal condensations of the fine granular matrix at various locations in the interior. PMID:9495038

  12. Sesquiterpenes and other constituents of Xylaria sp. NC1214, a fungal endophyte of the moss Hypnum sp.

    PubMed

    Wei, Han; Xu, Ya-Ming; Espinosa-Artiles, Patricia; Liu, Manping X; Luo, Jiang-Guang; U'Ren, Jana M; Elizabeth Arnold, A; Leslie Gunatilaka, A A

    2015-10-01

    Oxygenated guaiane-type sesquiterpenes, xylaguaianols A-D (1-4), an iso-cadinane-type sesquiterpene isocadinanol A (5), and an ?-pyrone 9-hydroxyxylarone (6), together with five known sesquiterpenes (7-11), and four known cytochalasins (12-15) were isolated from a culture broth of Xylaria sp. NC1214, a fungal endophyte of the moss Hypnum sp. The structures of all compounds were elucidated by the analysis of their spectroscopic data and relative configurations of 1-5 were determined with the help of NMR NOESY experiments. Cytochalasins C (12), D (13), and Q (14) were investigated for their cytotoxic activity against five tumor cell lines. Cytochalasin D showed significant cytotoxicity against all five cell lines, with IC50s ranging from 0.22 to 1.44?M, whereas cytochalasins C and Q exhibited moderate, but selective cytotoxicity. PMID:26324074

  13. Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)

    PubMed Central

    Olmstead, Ian L. D.; Bergamin, Amanda; Shears, Melanie J.; Dias, Daniel A.; Kentish, Sandra E.; Scales, Peter J.; Botté, Cyrille Y.; Callahan, Damien L.

    2014-01-01

    Many species of microalgae produce greatly enhanced amounts of triacylglycerides (TAGs), the key product for biodiesel production, in response to specific environmental stresses. Improvement of TAG production by microalgae through optimization of growth regimes is of great interest. This relies on understanding microalgal lipid metabolism in relation to stress response in particular the deprivation of nutrients that can induce enhanced TAG synthesis. In this study, a detailed investigation of changes in lipid composition in Chlorella sp. and Nannochloropsis sp. in response to nitrogen deprivation (N-deprivation) was performed to provide novel mechanistic insights into the lipidome during stress. As expected, an increase in TAGs and an overall decrease in polar lipids were observed. However, while most membrane lipid classes (phosphoglycerolipids and glycolipids) were found to decrease, the non-nitrogen containing phosphatidylglycerol levels increased considerably in both algae from initially low levels. Of particular significance, it was observed that the acyl composition of TAGs in Nannochloropsis sp. remain relatively constant, whereas Chlorella sp. showed greater variability following N-deprivation. In both algae the overall fatty acid profiles of the polar lipid classes were largely unaffected by N-deprivation, suggesting a specific FA profile for each compartment is maintained to enable continued function despite considerable reductions in the amount of these lipids. The changes observed in the overall fatty acid profile were due primarily to the decrease in proportion of polar lipids to TAGs. This study provides the most detailed lipidomic information on two different microalgae with utility in biodiesel production and nutraceutical industries and proposes the mechanisms for this rearrangement. This research also highlights the usefulness of the latest MS-based approaches for microalgae lipid research. PMID:25171084

  14. Gap junctional communication modulates gene transcription by altering the recruitment of Sp1 and Sp3 to connexin-response elements in osteoblast promoters

    NASA Technical Reports Server (NTRS)

    Stains, Joseph P.; Lecanda, Fernando; Screen, Joanne; Towler, Dwight A.; Civitelli, Roberto

    2003-01-01

    Loss-of-function mutations of gap junction proteins, connexins, represent a mechanism of disease in a variety of tissues. We have shown that recessive (gene deletion) or dominant (connexin45 overexpression) disruption of connexin43 function results in osteoblast dysfunction and abnormal expression of osteoblast genes, including down-regulation of osteocalcin transcription. To elucidate the molecular mechanisms of gap junction-sensitive transcriptional regulation, we systematically analyzed the rat osteocalcin promoter for sensitivity to gap junctional intercellular communication. We identified an Sp1/Sp3 containing complex that assembles on a minimal element in the -70 to -57 region of the osteocalcin promoter in a gap junction-dependent manner. This CT-rich connexin-response element is necessary and sufficient to confer gap junction sensitivity to the osteocalcin proximal promoter. Repression of osteocalcin transcription occurs as a result of displacement of the stimulatory Sp1 by the inhibitory Sp3 on the promoter when gap junctional communication is perturbed. Modulation of Sp1/Sp3 recruitment also occurs on the collagen Ialpha1 promoter and translates into gap junction-sensitive transcriptional control of collagen Ialpha1 gene expression. Thus, regulation of Sp1/Sp3 recruitment to the promoter may represent a potential general mechanism for transcriptional control of target genes by signals passing through gap junctions.

  15. Draft Genome Sequences of Facultative Methylotrophs, Gemmobacter sp. Strain LW1 and Mesorhizobium sp. Strain 1M-11, Isolated from Movile Cave, Romania

    PubMed Central

    Wischer, Daniela; Hillebrand-Voiculescu, Alexandra M.

    2015-01-01

    Facultative methylotrophs belonging to the genera Gemmobacter and Mesorhizobium were isolated from microbial mat and cave water samples obtained from the Movile Cave ecosystem. Both bacteria can utilize methylated amines as their sole carbon and nitrogen source. Here, we report the draft genome sequences of Gemmobacter sp. strain LW1 and Mesorhizobium sp. strain IM1. PMID:26586870

  16. The family Carabodidae (Acari, Oribatida) VIII. The genus Machadocepheus (first part) Machadocepheus leoneae sp. n. and Machadocepheus rachii sp. n. from Gabon

    PubMed Central

    Fernandez, Nestor; Theron, Pieter; Rollard, Christine; Leiva, Sergio

    2014-01-01

    Abstract The genus Machadocepheus, being one of the more complex genera of the Carabodidae family, is briefly outlined to demonstrate this complexity. Descriptions of two new species from Gabon, Machadocepheus leoneae sp. n. and Machadocepheus rachii sp. n. are given. PMID:25561826

  17. 75 FR 42411 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Daily Contract; SP-15 Financial Day...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ...Contract; SP-15 Financial Swap Real Time LMP-Peak Daily Contract; NP-15 Financial...contract; SP-15 Financial Swap Real Time LMP-Peak Daily (``SRP'') contract...an ECM with respect to a SPDC; at that time such an ECM becomes subject to all...

  18. Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction.

    PubMed

    Ghio, Silvina; Lorenzo, Gonzalo Sabarís Di; Lia, Verónica; Talia, Paola; Cataldi, Angel; Grasso, Daniel; Campos, Eleonora

    2012-01-01

    Prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases and hemicellulases that could be useful in second-generation bioethanol production. In this work, cellulolytic bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. There was a predominance of Gram positive isolates that belonged to the phyla Proteobacteria and Firmicutes. Many primary isolates with cellulolytic activity were not pure cultures. From these consortia, isolation of pure constituents was attempted in order to test the hypothesis whether microbial consortia are needed for full degradation of complex substrates. Two isolates, CB1-2-A-5 and VG-4-A-2, were obtained as the pure constituents of CB1-2 and VG-4 consortia, respectively. Based on 16S RNA sequence, they could be classified as Variovorax paradoxus and Paenibacillus alvei. Noteworthy, only VG-4 consortium showed measurable xylan degrading capacity and signs of filter paper degradation. However, no xylan or filter paper degrading capacities were observed for the pure cultures isolated from it, suggesting that other members of this consortium were necessary for these hydrolyzing activities. Our results indicated that Paenibacillus sp. and Variovorax sp. as well as VG-4 consortium, might be a useful source of hydrolytic enzymes. Moreover, although Variovorax sp. had been previously identified in metagenomic studies of cellulolytic communities, this is the first report on the isolation and characterization of this microorganism as a cellulolytic genus. PMID:23301200

  19. Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction

    PubMed Central

    Ghio, Silvina; Lorenzo, Gonzalo Sabarís Di; Lia, Verónica; Talia, Paola; Cataldi, Angel; Grasso, Daniel; Campos, Eleonora

    2012-01-01

    Prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases and hemicellulases that could be useful in second-generation bioethanol production. In this work, cellulolytic bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. There was a predominance of Gram positive isolates that belonged to the phyla Proteobacteria and Firmicutes. Many primary isolates with cellulolytic activity were not pure cultures. From these consortia, isolation of pure constituents was attempted in order to test the hypothesis whether microbial consortia are needed for full degradation of complex substrates. Two isolates, CB1-2-A-5 and VG-4-A-2, were obtained as the pure constituents of CB1-2 and VG-4 consortia, respectively. Based on 16S RNA sequence, they could be classified as Variovorax paradoxus and Paenibacillus alvei. Noteworthy, only VG-4 consortium showed measurable xylan degrading capacity and signs of filter paper degradation. However, no xylan or filter paper degrading capacities were observed for the pure cultures isolated from it, suggesting that other members of this consortium were necessary for these hydrolyzing activities. Our results indicated that Paenibacillus sp. and Variovorax sp. as well as VG-4 consortium, might be a useful source of hydrolytic enzymes. Moreover, although Variovorax sp. had been previously identified in metagenomic studies of cellulolytic communities, this is the first report on the isolation and characterization of this microorganism as a cellulolytic genus. PMID:23301200

  20. Maribacter spongiicola sp. nov. and Maribacter vaceletii sp. nov., isolated from marine sponges, and emended description of the genus Maribacter.

    PubMed

    Jackson, Stephen A; Kennedy, Jonathan; Morrissey, John P; O'Gara, Fergal; Dobson, Alan D W

    2015-07-01

    Two Gram-stain-negative, rod-shaped, orange, catalase- and oxidase-positive, non-motile bacteria, designated W13M1A(T) and W15M10(T), were isolated from the marine sponges Suberites carnosus and Leucosolenia sp., respectively, which were sampled from Lough Hyne, Co. Cork, Ireland. Analysis of the 16S rRNA gene sequences of these isolates revealed that they are members of the genus Maribacter, in the family Flavobacteriaceae of the phylum Bacteroidetes. The type strain most closely related to strain W13M1A(T) is Maribacter forsetii DSM 18668(T) with a gene sequence similarity of 96.5%. The closest related type strain to strain W15M10(T) is Maribacter orientalis DSM 16471(T) with a gene sequence similarity of 98.3%. Phylogenetic inference and phenotypic data combined indicate that the isolates represent two novel species of the genus Maribacter, for which the names Maribacter spongiicola sp. nov. with type strain W15M10(T) (?= NCIMB 14725(T) = DSM 25233(T)) and Maribacter vaceletii sp. nov. with type strain W13M1A(T) (?= NCIMB 14724(T) = DSM 25230(T)), are proposed. PMID:25833155

  1. Histone Deacetylase 1 Can Repress Transcription by Binding to Sp1

    PubMed Central

    Doetzlhofer, Angelika; Rotheneder, Hans; Lagger, Gerda; Koranda, Manfred; Kurtev, Vladislav; Brosch, Gerald; Wintersberger, Erhard; Seiser, Christian

    1999-01-01

    The members of the Sp1 transcription factor family can act as both negative and positive regulators of gene expression. Here we show that Sp1 can be a target for histone deacetylase 1 (HDAC1)-mediated transcriptional repression. The histone deacetylase inhibitor trichostatin A activates the chromosomally integrated murine thymidine kinase promoter in an Sp1-dependent manner. Coimmunoprecipitation experiments with Swiss 3T3 fibroblasts and 293 cells demonstrate that Sp1 and HDAC1 can be part of the same complex. The interaction between Sp1 and HDAC1 is direct and requires the carboxy-terminal domain of Sp1. Previously we have shown that the C terminus of Sp1 is necessary for the interaction with the transcription factor E2F1 (J. Karlseder, H. Rotheneder, and E. Wintersberger, Mol. Cell. Biol. 16:1659–1667, 1996). Coexpression of E2F1 interferes with HDAC1 binding to Sp1 and abolishes Sp1-mediated transcriptional repression. Our results indicate that one component of Sp1-dependent gene regulation involves competition between the transcriptional repressor HDAC1 and the transactivating factor E2F1. PMID:10409740

  2. Binding of the ubiquitous cellular transcription factors Sp1 and Sp3 to the ZI domains in the Epstein-Barr virus lytic switch BZLF1 gene promoter.

    PubMed

    Liu, S; Borras, A M; Liu, P; Suske, G; Speck, S H

    1997-02-01

    Induction of the Epstein-Barr virus lytic cycle in latently infected B cells requires the expression of the immediate-early lytic gene BZLF1. We have previously identified several cis-elements within the BZLF1 promoter that are required for induction by known inducers of the lytic cycle [E. Flemington and S. H. Speck (1990)J. Virol. 64, 1217-1226]. These include four elements termed the ZI domains (ZIA, ZIB, ZIC, and ZID) that share extensive homology and that have recently been shown to bind several cellular transcription factors [A. M. Borras, J. L. Strominger, and S. H. Speck (1996) J. Virol. 70, 3894-3901]. Here Sp1 and Sp3 are identified as the cellular factors present in crude B cell nuclear extract preparations that bind to the ZIC domain. In addition, three of the four complexes observed in electrophoretic mobility shift analyses employing probes containing either the ZIA or the ZID domains also represent Sp1 or Sp3 binding. Binding of Sp1 and Sp3 to the ZI domains was shown to be significantly weaker than binding of these factors to a consensus Sp1 site. A heterologous promoter construct containing three repeats of a consensus Sp1 site, cloned upstream of a single copy of the ZII (CREB/ AP1) element from the BZLF1 promoter linked to the beta-globin TATA box, exhibited phorbol ester inducibility. The latter observation was consistent with the functional behavior exhibited by a heterologous promoter construct containing multiple copies of the ZIC domain liked to the ZII element. However, the basal activity of the heterologous promoter construct driven by the consensus Sp1 sites was ca. 10-fold higher than that of the heterologous reporter construct containing multimerized ZIC sites. Thus, the low affinity of Sp1 binding to the ZI domains may contribute to the low-level basal activity of the BZLF1 promoter. PMID:9024805

  3. Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp. nov., isolated from industrial and haemodialysis water.

    PubMed

    Gomila, Margarita; Bowien, Botho; Falsen, Enevold; Moore, Edward R B; Lalucat, Jorge

    2007-11-01

    Three Gram-negative, rod-shaped, non-spore-forming bacteria (strains CCUG 52769T, CCUG 52770 and CCUG 52771) isolated from haemodialysis water were characterized taxonomically, together with five strains isolated from industrial waters (CCUG 52428, CCUG 52507, CCUG 52575T, CCUG 52590 and CCUG 52631). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the class Betaproteobacteria and were related to the genus Pelomonas, with 16S rRNA gene sequence similarities higher than 99% with the only species of the genus, Pelomonas saccharophila and to Pseudomonas sp. DSM 2583. The type strains of Mitsuaria chitosanitabida and Roseateles depolymerans were their closest neighbours (97.9 and 97.3% 16S rRNA gene sequence similarity, respectively). Phylogenetic analysis was also performed for the internally transcribed spacer region and for three genes [hoxG (hydrogenase), cbbL/cbbM (Rubisco) and nifH (nitrogenase)] relevant for the metabolism of the genus Pelomonas. DNA-DNA hybridization, major fatty acid composition and phenotypical analyses were carried out, which included the type strain of Pelomonas saccharophila obtained from different culture collections (ATCC 15946T, CCUG 32988T, DSM 654T, IAM 14368T and LMG 2256T), as well as M. chitosanitabida IAM 14711T and R. depolymerans CCUG 52219T. Results of DNA-DNA hybridization, physiological and biochemical tests supported the conclusion that strains CCUG 52769, CCUG 52770 and CCUG 52771 represent a homogeneous phylogenetic and genomic group, including strain DSM 2583, clearly differentiated from the industrial water isolates and from the Pelomonas saccharophila type strain. On the basis of phenotypic and genotypic characteristics, these strains belong to two novel species within the genus Pelomonas, for which the names Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are proposed. The type strains of Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are CCUG 52769T (=CECT 7234T) and CCUG 52575T (=CECT 7233T), respectively. PMID:17978231

  4. SP-100 nuclear space power systems with application to space commercialization

    NASA Technical Reports Server (NTRS)

    Smith, J. M.

    1990-01-01

    The technology of the SP-100 space nuclear power system program is compared to that of more familiar solar-power systems. The SP-100 program develops, validates, and demonstrates the technology for space nuclear power systems in the range of 10 to 1000 kilowatts electric for use in future military and civilian space missions. Mission applications, including earth orbiting platforms and lunar/Mars surface power, are enhanced or made possible by SP-100 technology. Attention is given to the SP-100 reference flight system design, the SP-100 nuclear reactor and nuclear-reactor shield, the platform-mounted, tethered, and free-flying reactors, and installation, operation, and disposal options, as well as lunar-Mars surface applications. The SP-100 is presented as one of the nuclear energy sources needed for long-life, compact, lightweight, continuous high power independent of solar orientation, specific orbits, or missions.

  5. Effect of salt stress on the physiology of Frankia sp strain CcI6.

    PubMed

    Oshone, Rediet; Mansour, Samira R; Tisa, Louis S

    2013-11-01

    Actinorhizal plants are able to overcome saline soils and reclaim land. Frankia sp strain CcI6 was isolated from nodules of Casuarina cunninghamiana found in Egypt. Phylogenetic analysis of Frankia sp. strain CcI6 revealed that the strain is closely related to Frankia sp. strain CcI3. The strain displays an elevated level of NaCl tolerance. Vesicle production and nitrogenase activity were also influenced by NaCl. PMID:24287648

  6. A 3-D time-dependent unstructured tetrahedral-mesh SP{sub N} method

    SciTech Connect

    Morel, J.E.; McGhee, J.M.; Larsen, E.W.

    1994-10-01

    We have developed a 3-D time-dependent multigroup SP{sub n} method for unstructured tetrahedral meshes. The SP{sub n} equations are expressed in a canonical form which allows them to be solved using standard diffusion solution techniques in conjunction with source iteration, diffusion-synthetic acceleration, and fission-source acceleration. A computational comparison of our SP{sub n} method with an even-parity S{sub n} method is given.

  7. Sphyrna gilberti sp. nov., a new hammerhead shark (Carcharhiniformes, Sphyrnidae) from the western Atlantic Ocean.

    PubMed

    Quattro, Joseph M; Driggers, William B; Grady, James M; Ulrich, Glenn F; Roberts, Mark A

    2013-01-01

    Sphyrna gilberti sp. nov. is described based on 54 specimens collected in the coastal waters of South Carolina, U.S.A. Morphologically, S. gilberti sp. nov. is separable from S. lewini (Griffith & Smith 1834) only in the number of precaudal vertebrae. Due to rarity of specimens and the highly migratory behavior of most sphyrnids, the range of S. gilberti sp. nov. is unknown. PMID:26146715

  8. A new cytosporone derivative from the endophytic fungus Cytospora sp.

    PubMed

    Takano, Tomoya; Koseki, Takuya; Koyama, Hiromasa; Shiono, Yoshihito

    2014-07-01

    Japanese oak wilt (JOW) is a tree disease caused by the fungus Raffaelea quercivora, which is vectored by the ambrosia beetle, Platypus quercivorus. In a screening study of the inhibitory active compounds from fungi, a new cytosporone analogue, compound 1, was isolated from the endophytic fungus Cytospora sp. TT-10 isolated from Japanese oak, together with the known compounds, integracin A (2), cytosporones N (3) and A (4). Their structures were determined by extensive 1D- and 2D-NMR spectroscopic and mass spectral analyses. Compound 1 was identified as 4,5-dihydroxy-3-heptylphthalide and named cytosporone E. Compounds 2 and 3 showed antimicrobial activity against Raffaelea quercivora. PMID:25230507

  9. Purification and characterization of biosurfactants from Nocardia sp. L-417.

    PubMed

    Kim, S H; Lim, E J; Lee, S O; Lee, J D; Lee, T H

    2000-06-01

    The Nocardia sp. L-417 strain grown with n-hexadecane as a carbon source produced two types of biosurfactant that have different characteristics. These biosurfactants were purified by procedures that included ammonium sulphate fractionation, chilled acetone and hexane treatments, silica-gel column chromatography and Sephadex LH-20 gel filtration. The purified biosurfactants were very stable over a broad range of pHs (2-12) and temperatures (100 degrees C, 3 h). The biosurfactant type I had strong properties as an emulsifying agent and as an emulsion-stabilizing agent, whereas type II had a strong ability to reduce surface tension. PMID:10814597

  10. Identification of trichostatin derivatives from Streptomyces sp. CPCC 203909.

    PubMed

    Chen, Minghua; Wu, Yexiang; He, Yi; Xu, Yanni; Li, Yongzhen; Li, Dongsheng; Feng, Tingting; Yu, Liyan; Hong, Bin; Jiang, Wei; Si, Shuyi

    2015-02-01

    Four new trichostatin analogues (1-4) and six known analogues have been isolated from the rice fermentation of the Streptomyces sp. CPCC 203909. The structures and absolute configurations of these compounds were determined by extensive spectroscopic analysis including 2D NMR and electronic circular dichroism (ECD) calculations based on the quantum-mechanical time-dependent density functional theory (TDDFT). Compounds 2, 5-7, 9, and 10 up-regulated the transcriptional activity of human high density lipoprotein receptor (CLA-1) with EC50 values of 0.38-78.83?M. PMID:25556102

  11. Amino Acid-Derived Metabolites from the Ascidian Aplidium sp.

    PubMed Central

    Won, Tae Hyung; Kim, Chang-Kwon; Lee, So-Hyoung; Rho, Boon Jo; Lee, Sang Kook; Oh, Dong-Chan; Oh, Ki-Bong; Shin, Jongheon

    2015-01-01

    Four new iodobenzene-containing dipeptides (1–4), a related bromotryptophan-containing dipeptide (5), and an iodophenethylamine (6) were isolated from the ascidian Aplidium sp. collected off the coast of Chuja-do, Korea. The structures of these novel compounds, designated as apliamides A–E (1–5) and apliamine A (6) were determined via combined spectroscopic analyses. The absolute configuration of the amino acid residue in 1 was determined by advanced Marfey’s analysis. Several of these compounds exhibited moderate cytotoxicity and significant inhibition against Na+/K+-ATPase (4). PMID:26087023

  12. Novel Adociaquinone Derivatives from the Indonesian Sponge Xestospongia sp.

    PubMed Central

    He, Fei; Mai, Linh H.; Longeon, Arlette; Copp, Brent R.; Loaëc, Nadège; Bescond, Amandine; Meijer, Laurent; Bourguet-Kondracki, Marie-Lise

    2015-01-01

    Seven new adociaquinone derivatives, xestoadociaquinones A (1a), B (1b), 14-carboxy-xestoquinol sulfate (2) and xestoadociaminals A–D (3a, 3c, 4a, 4c), together with seven known compounds (5–11) were isolated from an Indonesian marine sponge Xestospongia sp. Their structures were elucidated by extensive 1D and 2D NMR and mass spectrometric data. All the compounds were evaluated for their potential inhibitory activity against eight different protein kinases involved in cell proliferation, cancer, diabetes and neurodegenerative disorders as well as for their antioxidant and antibacterial activities. PMID:25927661

  13. Bispuupehenone from the South Chinese Sea sponge Dysidea sp.

    PubMed Central

    Qin, Song; Shi, Lei; Li, Jia; Guo, Yue-Wei

    2008-01-01

    Bispuupehenone, C42H54O6, formally results from dimerization of puupehenone, which is constructed of sesquiterpene and benzene units. Bispuupehenone was isolated from the South China Sea sponge Dysidea sp. and the single-crystal X-ray diffraction analysis confirmed the previously reported structure. The mol­ecule is located on a twofold axis and the dimerization forms two fused dibenzopyran systems related by symmetry. In the asymmetric unit, the two cyclohexane rings adopt chair conformations, while the two pyran rings adopt half-chair conformations. The relative stereochemistry and configurations for the ring junctions are in agreement with the structure reported previously. PMID:21202427

  14. Computational complexity of multiple sequence alignment with SP-score.

    PubMed

    Just, W

    2001-01-01

    It is shown that the multiple alignment problem with SP-score is NP-hard for each scoring matrix in a broad class M that includes most scoring matrices actually used in biological applications. The problem remains NP-hard even if sequences can only be shifted relative to each other and no internal gaps are allowed. It is also shown that there is a scoring matrix M(0) such that the multiple alignment problem for M(0) is MAX-SNP-hard, regardless of whether or not internal gaps are allowed. PMID:11747615

  15. Enzymatic transformation of stevioside using a ?-galactosidase from Sulfolobus sp.

    PubMed

    Wan, Hui-da; Xia, Yong-Mei

    2015-10-01

    Enzymatic hydrolysis and transgalactosylation of stevioside (St) were investigated using a ?-galactosidase from Sulfolobus sp. The hydrolysis yielded steviol as the main final product. Under the optimal transgalactosylation conditions, the highest conversion of stevioside was 87.3% with lactose as a donor, several galactosylated products (St-Gals) were obtained. Metal ions such as Na(+), K(2+), Ca(2+), Ba(2+), Mn(2+) and Mg(2+) (2 mM) did not affect the transgalactosylation activity, while Fe(2+), Fe(3+) and Cu(2+) reduced the transgalactosylation activity of ?-galactosidase to 64%, 33% and 18%, respectively. PMID:26242384

  16. Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803

    PubMed Central

    Selstam, Eva; Norling, Birgitta

    2015-01-01

    The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that “light” plasma membranes have a high carotenoid/protein ratio, when compared to “heavier” plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of ?-carotene precursor molecules and echinenone. PMID:26083372

  17. Polyketides from a marine-derived fungus Xylariaceae sp.

    PubMed

    Nong, Xu-Hua; Zheng, Zhi-Hui; Zhang, Xiao-Yong; Lu, Xin-Hua; Qi, Shu-Hua

    2013-05-01

    Eighteen polyketides (1-18) including six citrinin derivatives, two phenol derivatives, one cyclopentenone, two naphthol derivatives, and seven tetralone derivatives were isolated from the culture broth of a marine-derived fungal strain Xylariaceae sp. SCSGAF0086. Five of these compounds (1, 2, 8, 9, and 10) were new, and their structures were determined by spectroscopic methods. Compounds 4, 6, 7, and 17 showed enzyme-inhibitory activities towards several tested enzymes, and 6 and 7 showed strong antifouling activity against Bugula neritina larvae settlement. This is the first time that the antifouling and enzyme-inhibitory activities of these compounds has been reported. PMID:23697953

  18. A new ascochlorin derivative from Cylindrocarpon sp. FKI-4602.

    PubMed

    Kawaguchi, Mio; Fukuda, Takashi; Uchida, Ryuji; Nonaka, Kenichi; Masuma, Rokuro; Tomoda, Hiroshi

    2013-01-01

    Cylindrol A?, a new ascochlorin congener, was isolated along with 14 known compounds from the culture broth of Cylindrocarpon sp. FKI-4602 by solvent extraction, octadecylsilane column chromatography and HPLC. The structure of cylindrol A? was elucidated by spectral analyses, including NMR. The compound has an ascochlorin skeleton consisting of a resorcin aldehyde and a cyclohexanone moieties. Cylindrol A? showed moderate antimicrobial activity against Bacillus subtilis, Kocuria rhizophila, Mycobacterium smegmatis and Acholeplasma laidlawii. The biosynthetic pathway to cylindrol A? was deduced from the 14 isolated metabolites of the fungal strain. PMID:23168404

  19. Metabolism of 4-Chloronitrobenzene by the Yeast Rhodosporidium sp

    PubMed Central

    Corbett, Michael D.; Corbett, Bernadette R.

    1981-01-01

    The yeast Rhodosporidium sp. metabolized 4-chloronitrobenzene by a reductive pathway to give 4-chloroacetanilide and 4-chloro-2-hydroxyacetanilide as the major final metabolites. The intermediate production of 4-chloronitrosobenzene, 4-chlorophenylhydroxylamine, and 4-chloroaniline was demonstrated by high-pressure liquid chromatography. Additional studies with selected metabolites established that the metabolite 4-chloro-2-hydroxyacetanilide was produced by an initial Bamberger rearrangement of the hydroxylamine metabolite, followed by acetylation. Direct C hydroxylation of the aromatic ring was not observed in this species. No hydroxamic acid production was detected, even though significant concentrations of the nitroso and hydroxylamine precursors to this functional group were observed. PMID:16345757

  20. Arrhopalites potapovi sp. nov. (Collembola, Symphypleona) from Russia.

    PubMed

    Vargovitsh, Robert S

    2015-01-01

    A new springtail species of the family Arrhopalitidae, Arrhopalites potapovi sp. nov., is described. It belongs to the caecus species group characterized by 3,2,1,1,1 anterior setae on dens and separates from Arrhopalites caecus (Tullberg) and other congeners by strongly differentiated cuticular spines on sixth abdominal segment, shape of female subanal appendages and foot complex. It was found under the loose bark of trees from two localities along Lake Baikal and it is the second representative of the genus Arrhopalites Börner sensu stricto in Russia. PMID:25947839

  1. Genome sequence and description of Mannheimia massilioguelmaensis sp. nov.

    PubMed Central

    Hadjadj, L.; Bentorki, A.A.; Michelle, C.; Amoura, K.; Djahoudi, A.; Rolain, J.-M.

    2015-01-01

    Strain MG13T sp. nov. is the type strain of Mannheimia massilioguelmaensis, a new species within the genus Mannheimia. This strain was isolated from the exudate of a skin lesion of an Algerian man. Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae. Here we describe this organism, together with the complete genome sequence and annotation. The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes. PMID:26693284

  2. Protospirura siamensis n. sp. (Nematoda: Spiruridae) from rodents in Thailand.

    PubMed

    Ribas, Alexis; Veciana, Marina; Chaisiri, Kittipong; Morand, Serge

    2012-05-01

    A large number (828) of rodents belonging to nine genera (Bandicota, Berylmys, Chiropodomys, Hapalomys, Leopoldamys, Maxomys, Mus, Niviventer and Rattus) were trapped in four Thai provinces between 2008 and 2010. A new species of Protospirura (Nematoda: Spiruridae) was identified and described. Protospirura siamensis n. sp. was found in 10 rodent species from the four Thai provinces surveyed. The new species can be distinguished from known species of the genus by the arrangement of the papillae on the male and the morphology and length of the spicules. This is the first species of Protospirura to be described from South-East Asia. PMID:22488429

  3. Isospin symmetry breaking in an algebraic pairing Sp(4) model

    E-print Network

    K. D. Sviratcheva; A. I. Georgieva; J. P. Draayer

    2007-03-22

    An exactly solvable sp(4) algebraic approach extends beyond the traditional isospin conserving nuclear interaction to bring forward effects of isospin symmetry breaking and isospin mixing resulting from a two-body nuclear interaction that includes proton-neutron (pn) and like-particle isovector pairing correlations plus significant isoscalar pn interactions. The model yields an estimate for the extent to which isobaric analog 0+ states in light and medium mass nuclei may mix with one another and reveals possible, but still extremely weak, non-analog beta-decay transitions.

  4. Polysaccharide extraction from Spirulina sp. and its antioxidant capacity.

    PubMed

    Chaiklahan, Ratana; Chirasuwan, Nattayaporn; Triratana, Panya; Loha, Veara; Tia, Suvit; Bunnag, Boosya

    2013-07-01

    To optimize polysaccharide extraction from Spirulina sp., the effect of solid-to-liquid ratio, extraction temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that extraction temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, temperature of 90°C, and time of 120 min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45 mg GAE/g sample. PMID:23541559

  5. Cdc20 mediates D-box-dependent degradation of Sp100

    SciTech Connect

    Wang, Ran; Li, Ke-min; Zhou, Cai-hong; Xue, Jing-lun; Ji, Chao-neng; Chen, Jin-zhong

    2011-12-02

    Highlights: Black-Right-Pointing-Pointer Cdc20 is a co-activator of APC/C complex. Black-Right-Pointing-Pointer Cdc20 recruits Sp100 and mediates its degradation. Black-Right-Pointing-Pointer The D-box of Sp100 is required for Cdc20-mediated degradation. Black-Right-Pointing-Pointer Sp100 expresses consistently at both the mRNA and protein levels in cell cycle. -- Abstract: Cdc20 is a co-activator of the anaphase-promoting complex/cyclosome (APC/C complex), which recruits substrates at particular phases of the cell cycle and mediates their degradation. Sp100 is a PML-NB scaffold protein, which localizes to nuclear particles during interphase and disperses from them during mitosis, participates in viral resistance, transcriptional regulation, and apoptosis. However, its metabolism during the cell cycle has not yet been fully characterized. We found a putative D-box in Sp100 using the Eukaryotic Linear Motif (ELM) predictor database. The putative D-box of Sp100 was verified by mutational analysis. Overexpression of Cdc20 resulted in decreased levels of both endogenous Sp100 protein and overexpressed Sp100 mRNA in HEK 293 cells. Only an overexpressed D-box deletion mutant of Sp100 accumulated in HEK293 cells that also overexpressed Cdc20. Cdc20 knockdown by cdc20 specific siRNA resulted in increased Sp100 protein levels in cells. Furthermore, we discovered that the Cdc20 mediated degradation of Sp100 is diminished by the proteasome inhibitor MG132, which suggests that the ubiquitination pathway is involved in this process. However, unlike the other Cdc20 substrates, which display oscillating protein levels, the level of Sp100 protein remains constant throughout the cell cycle. Additionally, both overexpression and knockdown of endogenous Sp100 had no effect on the cell cycle. Our results suggested that sp100 is a novel substrate of Cdc20 and it is degraded by the ubiquitination pathway. The intact D-box of Sp100 was necessary for this process. These findings expand our knowledge of both Sp100 and Cdc20 as well as their role in ubiquitination.

  6. Aspirin Inhibits Colon Cancer Cell and Tumor Growth and Downregulates Specificity Protein (Sp) Transcription Factors

    PubMed Central

    Pathi, Satya; Jutooru, Indira; Chadalapaka, Gayathri; Nair, Vijayalekshmi; Lee, Syng-Ook; Safe, Stephen

    2012-01-01

    Acetylsalicylic acid (aspirin) is highly effective for treating colon cancer patients postdiagnosis; however, the mechanisms of action of aspirin in colon cancer are not well defined. Aspirin and its major metabolite sodium salicylate induced apoptosis and decreased colon cancer cell growth and the sodium salt of aspirin also inhibited tumor growth in an athymic nude mouse xenograft model. Colon cancer cell growth inhibition was accompanied by downregulation of Sp1, Sp3 and Sp4 proteins and decreased expression of Sp-regulated gene products including bcl-2, survivin, VEGF, VEGFR1, cyclin D1, c-MET and p65 (NF?B). Moreover, we also showed by RNA interference that ?-catenin, an important target of aspirin in some studies, is an Sp-regulated gene. Aspirin induced nuclear caspase-dependent cleavage of Sp1, Sp3 and Sp4 proteins and this response was related to sequestration of zinc ions since addition of zinc sulfate blocked aspirin-mediated apoptosis and repression of Sp proteins. The results demonstrate an important underlying mechanism of action of aspirin as an anticancer agent and, based on the rapid metabolism of aspirin to salicylate in humans and the high salicylate/aspirin ratios in serum, it is likely that the anticancer activity of aspirin is also due to the salicylate metabolite. PMID:23110215

  7. NIST SP 1029 Structure of a folded catalytic RNA (ribozyme) of the

    E-print Network

    in Thin Sheet Metal. . . . . . . . . . . . . . . . . . . . 20 Competing Magnetic Phases in the "KagoméNIST SP 1029 #12;Structure of a folded catalytic RNA (ribozyme) of the Azoarcus bacterium obtained

  8. Wheat TaSP gene improves salt tolerance in transgenic Arabidopsis thaliana.

    PubMed

    Ma, Xiaoli; Cui, Weina; Liang, Wenji; Huang, Zhanjing

    2015-12-01

    A novel salt-induced gene with unknown functions was cloned through analysis of gene expression profile of a salt-tolerant wheat mutant RH8706-49 under salt stress. The gene was named Triticum aestivum salt-related protein (TaSP) and deposited in GenBank (Accession No. KF307326). Quantitative polymerase chain reaction (qPCR) results showed that TaSP expression was induced under salt, abscisic acid (ABA), and polyethylene glycol (PEG) stresses. Subcellular localization revealed that TaSP was mainly localized in cell membrane. Overexpression of TaSP in Arabidopsis could improve salt tolerance of 35S::TaSP transgenic Arabidopsis. 35S::TaSP transgenic Arabidopsis lines after salt stress presented better physiological indexes than the control group. In the non-invasive micro-test (NMT), an evident Na(+) excretion was observed at the root tip of salt-stressed 35S::TaSP transgenic Arabidopsis. TaSP promoter was cloned, and its beta-glucuronidase (GUS) activities before and after ABA, salt, cold, heat, and salicylic acid (SA) stresses were determined. Full-length TaSP promoter contained ABA and salt response elements. PMID:26476792

  9. Protective Effect of Sundakai (Solanum torvum) Seed Protein (SP) Against Oxidative Membrane Damage in Human Erythrocytes.

    PubMed

    Sivapriya, M; Gowda, S S Thammanna; Srinivas, Leela

    2015-12-01

    Lipid peroxidation by ROS at the membrane level disturbs the inherit integrity of components activating subsequent alterations in the function. In this study, the protective effect of purified Sundakai (Solanum torvum) seed protein (SP) was tested against oxidative membrane damage in erythrocyte membrane. SP prevented oxidative RBC lysis induced by pro-oxidants; Fe:As (2:20 ?mol), periodate (0.4 mM), and t-BOOH (1 mM) up to 86, 81, and 86 %, respectively. Further, SP prevented the Fe:As-induced K(+) leakage up to the tune of 95 %. The inhibition offered by SP on K(+) leakage was comparable to inhibition offered by quinine sulfate, a known K(+) channel blocker. SP dose dependently restored Na(+)K(+) ATPase and Ca(2+)Mg(2+) ATPase activities in erythrocyte membrane. The restoration of ATPase activity by SP was two times more than standard antioxidants BHA and ?-tocopherol. Besides, SP at 1.6 ?mol restored the membrane proteins over Fe:As induction when analyzed by SDS-PAGE, which was comparable to protection offered by BHA. In conclusion, SP is an effective antioxidant in preventing oxidative membrane damage and associated functions mediated by ROS. As SP is non-toxic, it can be used as an effective bioprotective antioxidant agent to cellular components. PMID:26374653

  10. UNIPASS for AvSP? A Broader View

    NASA Technical Reports Server (NTRS)

    Wu, N. Eva

    2001-01-01

    UNIPASS is a general-purpose probabilistic computer program consisting of three major modules, including preprocessor, solver and postprocessor. UNIPASS contains a user-friendly Graphical User Interface (GUI), numerous state-of-the-art probabilistic analysis techniques, a large library of statistical distributions and a function module with a large library of support functions that can easily define any complex limit-state function in a scripting FORTRAN-like syntax format. Its inverse probability analysis and sensitivities analysis capabilities make it a powerful design aid in any product cycle. Its precise numerical analysis engine is accurate enough to push the failure probabilities of a design to well below 10 (exp -50). UNIPASS is equipped with advanced artificial intelligence that is designed to handle systems with an essentially unlimited number of random variables with ease and efficiency. Its modular arrangement allows you to tailor an analysis to the desired level of accuracy and efficiency. The depth and comprehensiveness of UNIPASS are built upon the decades of experience and expertise of industry leaders including Boeing Aircraft, NASA and the DoD. Its rich content also makes UNIPASS a valuable instructional tool for random processes and probabilistic mechanics. The topics include: 1) Reliability in AvSP; 2) Role of UNIPASS in AvSP; and 3) Examples. This paper is in viewgraph form.

  11. TRPV1 and SP: key elements for sepsis outcome?

    PubMed Central

    Bodkin, Jennifer Victoria; Fernandes, Elizabeth Soares

    2013-01-01

    Sensory neurons play important roles in many disorders, including inflammatory diseases, such as sepsis. Sepsis is a potentially lethal systemic inflammatory reaction to a local bacterial infection, affecting thousands of patients annually. Although associated with a high mortality rate, sepsis outcome depends on the severity of systemic inflammation, which can be directly influenced by several factors, including the immune response of the patient. Currently, there is a lack of effective drugs to treat sepsis, and thus there is a need to develop new drugs to improve sepsis outcome. Several mediators involved in the formation of sepsis have now been identified, but the mechanisms underlying the pathology remain poorly understood. The transient receptor potential vanilloid 1 (TRPV1) receptor and the neuropeptide substance P (SP) have recently been demonstrated as important targets for sepsis and are located on sensory neurones and non-neuronal cells. Herein, we highlight and review the importance of sensory neurones for the modulation of sepsis, with specific focus on recent findings relating to TRPV1 and SP, with their distinct abilities to alter the transition from local to systemic inflammation and also modify the overall sepsis outcome. We also emphasize the protective role of TRPV1 in this context. LINKED ARTICLES This article is part of a themed section on Neuropeptides. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2013.170.issue-7 PMID:23145480

  12. Lubrication by the red microalgae Porphyridium sp. polysaccharide

    NASA Astrophysics Data System (ADS)

    Gourdon, Delphine; Lin, Qi; Golan, Yuval

    2004-03-01

    Using a surface forces apparatus (SFA) we have investigated the effects of normal load (compression) and shear on the tribological properties of mica surfaces bearing Porphyridium sp. (a common naturally-occurring polysaccharide of the red microalgae, which molecular weight has been estimated to be 2-7 x 106 daltons) adsorbed from aqueous solution. The friction forces were monitored as a function of time, shearing distance and driving velocity, at different loads and for different polysaccharide concentrations. The friction results indicate a very low coefficient of friction (? <0.01) at low compressions, increasing only to ? =0.015 at pressures of 10 MPa. Its low friction, its ability to resist to high pressure, as well as its weak (logarithmic) dependency of friction on sliding velocity make the Porphyridium sp. a good candidate for biolubrication (e.g. human joint lubrication). Atomic force microscopy measurements were also performed on each surface before and after a shearing experiment and showed ordering of the biopolymer chain molecules into layers over length-scales of several microns.

  13. SpIES:The Spitzer IRAC Equatorial Survey

    NASA Astrophysics Data System (ADS)

    Timlin, John; Ross, Nicholas; Richards, Gordon T.; Lacy, Mark; Bauer, Franz E.; Brandt, W. Niel; Fan, Xiaohui; Haggard, Daryl; Makler, Martin; Myers, Adam D.; Strauss, Michael A.; Urry, C. Megan; SpIES Team

    2015-01-01

    The Spitzer-IRAC Equatorial Survey, SpIES, is an Exploration Science program using Warm Spitzer to map over 100deg^2 of the SDSS Stripe 82 field, and is the largest extragalactic area surveyed by Spitzer. The primary science drivers are: the measurement of z>3 quasar clustering and the luminosity function in order to test different "AGN feedback'' models; to identify obscured AGN (and take advantage of the wide range of multi-wavelength, multi-epoch ancillary data on the Stripe 82 field); to identify z>6 quasars, and to support other wide-field ancillary science. With our observations very recently completed, we present the first preliminary science results from SpIES. This work is based [in part] on observations made with the Spitzer Space Telescope, which is operated by the Jet Propulsion Laboratory, California Institute of Technology under a contract with NASA. Support for this work was provided by NASA through an award issued by JPL/Caltech.

  14. SP-100 operational life model. Fiscal Year 1990 annual report

    SciTech Connect

    Ewell, R.; Awaya, H.

    1990-12-14

    This report covers the initial year`s effort in the development of an Operational Life Model (OLM) for the SP-100 Space Reactor Power System. The initial step undertaken in developing the OLM was to review all available documentation from GE on their plans for the OLM and on the degradation and failure mechanisms envisioned for the SP-100. In addition, the DEGRA code developed at JPL, which modelled the degradation of the General Purpose Heat Source based Radioisotope Thermoelectric Generator (GPHS-RTG), was reviewed. Based on the review of the degradation and failure mechanisms, a list of the most pertinent degradation effects along with their key degradation mechanisms was compiled. This was done as a way of separating the mechanisms from the effects and allowing all of the effects to be incorporated into the OLM. The emphasis was on parameters which will tend to change performance as a function of time and not on those that are simply failures without any prior degradation.

  15. Degradation of a Sodium Acrylate Oligomer by an Arthrobacter sp

    PubMed Central

    Hayashi, Takaya; Mukouyama, Masaharu; Sakano, Kouichi; Tani, Yoshiki

    1993-01-01

    Arthrobacter sp. strain NO-18 was first isolated from soil as a bacterium which could degrade the sodium acrylate oligomer and utilize it as the sole source of carbon. When 0.2% (wt/wt) oligomer was added to the culture medium, the acrylate oligomer was found to be degraded by 70 to 80% in 2 weeks, using gel permeation chromatography. To determine the maximum molecular weight for biodegradation, the degradation test was done with the hexamer, heptamer, and octamer, which were separated from the oligomer mixture by fractional gel permeation chromatography. The hexamer and heptamer were consumed to the extents of 58 and 36%, respectively, in 2 weeks, but the octamer was not degraded. Oligomers with three different terminal groups were synthesized to examine the effect of the different terminal groups on biodegradation, but few differences were found. Arthrobacter sp. NO-18 assimilated acrylic acid, propionic acid, glutaric acid, 2-methylglutaric acid, and 1,3,5-pentanetricarboxylic acid. Degradation of the acrylic unit structure by this strain is discussed. PMID:8517751

  16. Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5

    PubMed Central

    Vigneswari, S.; Lee, T. S.; Bhubalan, Kesaven; Amirul, A. A.

    2015-01-01

    Bacteria capable of degrading polyhydroxyalkanoates (PHA) by secreting extracellular depolymerase enzymes were isolated from water and soil samples collected from various environments in Malaysia. A total of 8 potential degraders exhibited clear zones on poly(3-hydroxybutyrate) [P(3HB)] based agar, indicating the presence of extracellular PHA depolymerase. Among the isolates, DP5 exhibited the largest clearing zone with a degradation index of 6.0. The highest degradation activity of P(3HB) was also observed with depolymerase enzyme of DP5 in mineral salt medium containing P(3HB). Based on biochemical characterization and 16S rRNA cloning and sequencing, isolate DP5 was found to belong to the genus Acidovorax and subsequently named as Acidovorax sp. DP5. The highest extracellular depolymerase enzyme activity was achieved when 0.25%?(w/v) of P(3HB) and 1?g/L of urea were used as carbon and nitrogen source, respectively, in the culture media. The most suitable assay condition of the depolymerase enzyme in response to pH and temperature was tested. The depolymerase produced by strain Acidovorax sp. DP5 showed high percentage of degradation with P(3HB) films in an alkaline condition with pH 9 and at a temperature of 40°C. PMID:26664741

  17. SP-100 power system conceptual design for lunar base applications

    NASA Technical Reports Server (NTRS)

    Mason, Lee S.; Bloomfield, Harvey S.; Hainley, Donald C.

    1989-01-01

    A conceptual design is presented for a nuclear power system utilizing an SP-100 reactor and multiple Stirling cycle engines for operation on the lunar surface. Based on the results of this study, it was concluded that this power plant could be a viable option for an evolutionary lunar base. The design concept consists of a 2500 kWt (kilowatt thermal) SP-100 reactor coupled to eight free-piston Stirling engines. Two of the engines are held in reserve to provide conversion system redundancy. The remaining engines operate at 91.7 percent of their rated capacity of 150 kWe. The design power level for this system is 825 kWe. Each engine has a pumped heat-rejection loop connected to a heat pipe radiator. Power system performance, sizing, layout configurations, shielding options, and transmission line characteristics are described. System components and integration options are compared for safety, high performance, low mass, and ease of assembly. The power plant was integrated with a proposed human lunar base concept to ensure mission compatibility. This study should be considered a preliminary investigation; further studies are planned to investigate the effect of different technologies on this baseline design.

  18. Carbyne with finite length: The one-dimensional sp carbon

    PubMed Central

    Pan, Bitao; Xiao, Jun; Li, Jiling; Liu, Pu; Wang, Chengxin; Yang, Guowei

    2015-01-01

    Carbyne is the one-dimensional allotrope of carbon composed of sp-hybridized carbon atoms. Definitive evidence for carbyne has remained elusive despite its synthesis and preparation in the laboratory. Given the remarkable technological breakthroughs offered by other allotropes of carbon, including diamond, graphite, fullerenes, carbon nanotubes, and graphene, interest in carbyne and its unusual potential properties remains intense. We report the first synthesis of carbyne with finite length, which is clearly composed of alternating single bonds and triple bonds, using a novel process involving laser ablation in liquid. Spectroscopic analyses confirm that the product is the structure of sp hybridization with alternating carbon-carbon single bonds and triple bonds and capped by hydrogen. We observe purple-blue fluorescence emissions from the gap between the highest occupied molecular orbital and the lowest unoccupied molecular orbital of carbyne. Condensed-phase carbyne crystals have a hexagonal lattice and resemble the white crystalline powder produced by drying a carbyne solution. We also establish that the combination of gold and alcohol is crucial to carbyne formation because carbon-hydrogen bonds can be cleaved with the help of gold catalysts under the favorable thermodynamic environment provided by laser ablation in liquid and because the unique configuration of two carbon atoms in an alcohol molecule matches the elementary entity of carbyne. This laboratory synthesis of carbyne will enable the exploration of its properties and applications. PMID:26601318

  19. Polyhydroxybutyrate particles in Synechocystis sp PCC 6803: facts and fiction

    SciTech Connect

    Tsang, TK; Roberson, RW; Vermaas, WFJ

    2013-09-20

    Transmission electron microscopy has been used to identify poly-3-hydroxybutyrate (PHB) granules in cyanobacteria for over 40 years. Spherical inclusions inside the cell that are electron-transparent and/or slightly electron-dense and that are found in transmission electron micrographs of cyanobacteria are generally assumed to be PHB granules. The aim of this study was to test this assumption in different strains of the cyanobacterium Synechocystis sp. PCC 6803. Inclusions that resemble PHB granules were present in strains lacking a pair of genes essential for PHB synthesis and in wild-type cells under conditions that no PHB granules could be detected by fluorescence staining of PHB. Indeed, in these cells PHB could not be demonstrated chemically by GC/MS either. Based on the results gathered, it is concluded that not all the slightly electron-dense spherical inclusions are PHB granules in Synechocystis sp. PCC 6803. This result is potentially applicable to other cyanobacteria. Alternate assignments for these inclusions are discussed.

  20. Description of Hemicycliophora biosphaera n. sp. from Arizona (Nemata: Criconematidae)

    PubMed Central

    Chitambar, J. J.; Mahato, T. R.; McClure, M. A.; Marino, B. D. V.

    1997-01-01

    Hemicycliophora biosphaera n. sp. (Nemata: Criconematidae) was found in soil from a fallow field plot within the Biosphere 2 Center, Oracle, Arizona. The nematode species is characterized by continuous and irregular breaks in transverse striae in the lateral field, smooth annules, a rounded-truncate lip region with rounded anterior margins, three lip annules, first labial annule elevated and widened laterally, dome-shaped and elevated labial disc, stylet length (76-97 (?m), VA%T value (30-59), 234-273 body annules, and tail with a terminus offset, cylindrical to slightly conoid digit. Hemicycliophora biosphaera n. sp. most closely resembles H. armandae but differs from it in body width (30-39 vs. 38-54 ?m), stylet length (76-97 vs. 95-119 ?m), greater number of annules between the excretory pore and esophagus base (4-16 vs. 2), length of the tail terminal spike (16-28 vs. 32 ?m), lower Rvan value (9-15 vs. 16), and indistinct spetanatheca vs. distinct spermatheca. PMID:19274166

  1. High-quality permanent draft genome sequence of Bradyrhizobium sp. strain WSM1743 - an effective microsymbiont of an Indigofera sp. growing in Australia.

    PubMed

    Eshraghi, Leila; De Meyer, Sofie E; Tian, Rui; Seshadri, Rekha; Ivanova, Natalia; Pati, Amrita; Markowitz, Victor; Woyke, Tanja; Kyrpides, Nikos C; Tiwari, Ravi; Yates, Ron; Howieson, John; Reeve, Wayne

    2015-01-01

    Bradyrhizobium sp. strain WSM1743 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of an Indigofera sp. WSM1743 was isolated from a nodule recovered from the roots of an Indigofera sp. growing 20 km north of Carnarvon in Australia. It is slow growing, tolerates up to 1 % NaCl and is capable of growth at 37 °C. Here we describe the features of Bradyrhizobium sp. strain WSM1743, together with genome sequence information and its annotation. The 8,341,956 bp high-quality permanent draft genome is arranged into 163 scaffolds and 167 contigs, contains 7908 protein-coding genes and 75 RNA-only encoding genes and was sequenced as part of the Root Nodule Bacteria chapter of the Genomic Encyclopedia of Bacteria and Archaea project. PMID:26512312

  2. Intermolecular amidation of unactivated sp2 and sp2 C-H bonds via palladium-catalyzed cascade C-H activation/nitrene insertion.

    PubMed

    Thu, Hung-Yat; Yu, Wing-Yiu; Che, Chi-Ming

    2006-07-19

    This communication describes the Pd(OAc)2-catalyzed intermolecular amidation reactions of unactivated sp2 and sp3 C-H bonds using primary amides and potassium persulfate. The substrates containing a pendent oxime or pyridine group were amidated with excellent chemo- and regioselectivities. It is noteworthy that reactive C-X bonds were well-tolerated and a variety of primary amides can be effective nucleophiles for the Pd-catalyzed C-H amidation reactions. For the reaction of unactivated sp3 C-H bonds, beta-amidation of 1 degrees sp3 C-H bonds versus 2 degrees C-H bonds is preferred. The catalytic reaction is initiated by chelation-assisted cyclopalladation involving C-H bond activation. Preliminary mechanistic study suggested that the persulfate oxidation of primary amides should generate reactive nitrene species, which then reacted with the cyclopalladated complex. PMID:16834374

  3. Domain analysis for estrogen receptor/Sp1-mediated transactivation and detection of estrogen receptor/Sp1 protein interactions in living cells 

    E-print Network

    Kim, KyoungHyun

    2005-11-01

    Estrogen Receptor ? (ER?)/Sp1 activation of GC-rich gene promoters in breast cancer cells is dependent, in part, on the activation function 1 (AF1) of ER?. This study investigates contributions of the DNA binding domain ...

  4. Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit.

    PubMed

    Cotta, Michael A; Whitehead, Terence R; Falsen, Enevold; Moore, Edward; Lawson, Paul A

    2013-01-01

    A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular genetic methods was performed on six strains of unknown Gram-positive, nonspore-forming, facultative anaerobic coccus-shaped bacteria isolated from a swine-manure storage pit. On the basis of the 16S rRNA, RNA polymerase ?-subunit (rpoA) and 60 kDa chaperonin (cpn60) gene sequence analyses, it was shown that all the isolates were enterococci but formed two separate lines of descent. Pairwise 16S rRNA gene sequence comparisons demonstrated that the two novel organisms were most closely related to each other (97.9 %) and to Enterococcus aquimarinus (97.8 %). Both organisms contained major amounts of C(16:0), C(16:1) ?7c, C(16:1) ?7c, and C(18:1) ?7c/12t/9t as the major cellular fatty acids. Based on biochemical, chemotaxonomic and phylogenetic evidence, the names Enterococcus lemanii sp. nov. (type strain PC32(T) = CCUG 61260(T) = NRRL B-59661(T)) PPC27A = CCUG 61369; PPC38 = CCUG 61261 [corrected] and Enterococcus eurekensis sp. nov. (type strain PC4B(T) = CCUG 61259(T) = NRRL B-59662(T)) PPC15 = CCUG 61368; PPC107 = CCUG 61372 [corrected] are proposed for these hitherto undescribed species. PMID:22872431

  5. Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica

    USGS Publications Warehouse

    Rodriguez, Russell J.; Connell, L.; Redman, R.; Barrett, A.; Iszard, M.; Fonseca, A.

    2010-01-01

    During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales (Tremellomycetes, Agaricomycotina), but did not correspond to any of the hitherto recognized species. Two novel species, Dioszegia antarctica sp. nov. (type strain ANT-03-116T =CBS 10920T =PYCC 5970T) and Dioszegia cryoxerica sp. nov. (type strain ANT-03-071T =CBS 10919T =PYCC 5967T), are described to accommodate ten and three of these strains, respectively. Analysis of ITS sequences demonstrated intrastrain sequence heterogeneity in D. cryoxerica. The latter species is also notable for producing true hyphae with clamp connections and haustoria. However, no sexual structures were observed. The two novel species can be considered obligate psychrophiles, since they failed to grow above 20?°C and grew best between 10 and 15?°C.

  6. Sphingomonas oryziterrae sp. nov. and Sphingomonas jinjuensis sp. nov. isolated from rhizosphere soil of rice (Oryza sativa L.).

    PubMed

    Chung, Eu Jin; Jo, Eun Ju; Yoon, Hwan Sik; Song, Geun Cheol; Jeon, Che Ok; Chung, Young Ryun

    2011-10-01

    Two Gram-reaction-negative, yellow-orange-pigmented, rod-shaped bacterial strains, designated YC6722(T) and YC6723(T), were isolated from rhizosphere soil samples collected from rice fields in Jinju, Korea. Strains YC6722(T) and YC6723(T) grew optimally at 25-30 °C and at pH 7.0-8.5. Phylogenetic analyses of 16S rRNA gene sequences showed that strain YC6722(T) was most closely related to Sphingomonas jaspsi TDMA-16(T) (96.6?% sequence similarity) and strain YC6723(T) was related most closely to Sphingomonas aquatilis JSS7(T) (96.9?%). The two strains contained ubiquinone-10 (Q-10) as the major respiratory quinone system and sym-homospermidine as the major polyamine. The G+C contents of the genomic DNA of strains YC6722(T) and YC6723(T) were 63.3 and 61.0 mol%, respectively. The major fatty acid was C(18?:?1)?7c. The polar lipids detected in the two strains were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid, phosphatidyldimethylethanolamine and other unknown lipids. On the basis of their phylogenetic positions, and their biochemical and phenotypic characteristics, strains YC6722(T) and YC6723(T) represent two novel species of the genus Sphingomonas, for which the names Sphingomonas oryziterrae sp. nov. (?=?KCTC 22476(T) ?=?DSM 21455(T)) and Sphingomonas jinjuensis sp. nov. (KCTC 22477(T) ?=?DSM 21457(T)) are proposed. PMID:21057052

  7. Ctenascarophis lesteri n. sp. and Prospinitectus exiguus n. sp. (Nematoda: Cystidicolidae) from the skipjack tuna, Katsuwonus pelamis.

    PubMed

    Crites, J L; Overstreet, R M; Maung, M

    1993-12-01

    Two cystidicolid nematodes, Ctenascarophis lesteri n. sp. and Prospinitectus exiguus n. sp., are described from the skipjack tuna, Katsuwonus pelamis, from Fiji, New Caledonia, Marquesas Islands, New Zealand, Papua New Guinea, and Puerto Rico. The former species differs from Ctenascarophis gastricus, the only other member of the genus, by having comb rows extending farther posterior than two-thirds the body length, to the anus in the female; it also has differently distributed spines on each comb, with a maximum of 32 rather than 8. The male has 7 rows of precloacal longitudinal crests; 3 pairs of precloacal, 1 pair adanal, and 6 pairs postcloacal papillae; and a spicule ratio of 1:3.4-1:5.0. The latter species differs from Prospinitectus mollis, the only other species in the genus, by being less than one-half as long, 3.5-5.9 mm long; with a proportionally longer esophagus; fewer spines per spine ring, a maximum of 49 compared with 100; deirids that lack spinules; males with an unornamented precloacal cuticle as well as a different number and distribution of caudal papillae, 2 precloacal and 7 postcloacal; and a smaller spicule ratio, 1:4 rather than 1:5-1:7. PMID:8277376

  8. Indigoids Biosynthesis from Indole by Two Phenol-Degrading Strains, Pseudomonas sp. PI1 and Acinetobacter sp. PI2.

    PubMed

    Wang, Jing; Zhang, Xuwang; Fan, Jiangli; Zhang, Zhaojing; Ma, Qiao; Peng, Xiaojun

    2015-07-01

    In this study, two phenol-degrading bacterial strains, designated as PI1 and PI2, were isolated from activated sludge for the production of indigoids from indole. According to the 16S ribosomal RNA (rRNA) gene sequence analysis, strains PI1 and PI2 were identified as Pseudomonas sp. and Acinetobacter sp., respectively. Liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) was applied to analyze the metabolites during the biotransformation of indole by the phenol-degrading strains. The results indicated that both strains could catalyze the formation of four indigoids with the same prominent molecular ion (M-H)(-) peak at m/z 261.067 and molecular formula of C16H10N2O2, including indigo and a purple product, 2-(7-oxo-1H-indol-6(7H)-ylidene) indolin-3-one. Isatin and 7-hydroxyindole were detected as the intermediates. Thus, the possible pathways for the production of indigoids from indole were proposed. Subsequently, the optimal conditions for the production of indigo from indole were determined using response surface methodology, and 11.82?±?0.30 and 17.19?±?0.49 mg/L indigo were produced by strains PI1 and PI2, respectively. The present study should provide potential candidates for microbial production of indigoids. PMID:25926013

  9. Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov., thermophilic anaerobic representatives of the phylum Planctomycetes.

    PubMed

    Slobodkina, Galina B; Kovaleva, Olga L; Miroshnichenko, Margarita L; Slobodkin, Alexander I; Kolganova, Tatyana V; Novikov, Andrei A; van Heerden, Esta; Bonch-Osmolovskaya, Elizaveta A

    2015-03-01

    Two novel strains of thermophilic planctomycetes were recovered from terrestrial and subterranean habitats. Strain R1(T) was isolated from a hot spring (Kunashir Island, Russia) and strain SBP2(T) was isolated from a deep gold mine (South Africa). Both isolates grew in the temperature range 30-60 °C and pH range 5.0-8.0. Strain R1(T) grew optimally at 60 °C and pH 6.0-6.5; for SBP2(T) optimal conditions were at 52 °C and pH 7.5-8.0. Both strains were capable of anaerobic respiration with nitrate and nitrite as electron acceptors as well as of microaerobic growth. They also could grow by fermentation of mono-, di- and polysaccharides. Based on their phylogenetic position and phenotypic features we suggest that the new isolates represent two novel species belonging to a new genus in the order Planctomycetales, for which the names Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov. are proposed. The type strain of Thermogutta terrifontis, the type species of the genus, is R1(T) (?=?DSM 26237(T)?=?VKM B-2805(T)), and the type strain of Thermogutta hypogea is SBP2(T) (?=?JCM 19991(T)?=?VKM B-2782(T)). PMID:25479950

  10. Isolation of Arthrobacter spp. from clinical specimens and description of Arthrobacter cumminsii sp. nov. and Arthrobacter woluwensis sp. nov.

    PubMed Central

    Funke, G; Hutson, R A; Bernard, K A; Pfyffer, G E; Wauters, G; Collins, M D

    1996-01-01

    Arthrobacter spp. are very widely distributed in the environment (e.g., soil) but have not been described as causing disease in humans. Over a 6-year period, two reference laboratories isolated or received 11 strains which were eventually identified as belonging to the genus Arthrobacter. These strains had been initially identified as Centers for Disease Control and Prevention coryneform group B-1 and B-3 bacteria (whitishgrayish colonies of 2 mm or greater in diameter after 24 h of incubation, respiratory metabolism, absent or weak acid production from sugars, and hydrolysis of gelatin). However, chemotaxonomic investigations revealed lysine as the diamino acid of the cell wall and the presence of branched cellular fatty acids (with anteiso-pentadecanoic acid predominating) which was compatible with an assignment of the 11 isolates to the genus Arthrobacter only. Peptidoglycan and 16S rRNA gene sequence analyses demonstrated that three of the strains studied were representatives of a new Arthrobacter species for which the name Arthrobacter cumminsii sp. nov. is proposed and that one other strain represented a second new Arthrobacter species for which the name Arthrobacter woluwensis sp. nov. is proposed. This report is the first on the isolation of Arthrobacter spp. from clinical specimens. PMID:8880479

  11. Impacts of varying light regimes on phycobiliproteins of Nostoc sp. HKAR-2 and Nostoc sp. HKAR-11 isolated from diverse habitats.

    PubMed

    Kannaujiya, Vinod K; Sinha, Rajeshwar P

    2015-11-01

    The adaptability of cyanobacteria in diverse habitats is an important factor to withstand harsh conditions. In the present investigation, the impacts of photosynthetically active radiation (PAR; 400-700 nm), ultraviolet-B (UV-B; 280-315 nm), and PAR?+?UV-B radiations on two cyanobacteria viz., Nostoc sp. HKAR-2 and Nostoc sp. HKAR-11 inhabiting diverse habitats such as hot springs and rice fields, respectively, were studied. Cell viability was about 14 % in Nostoc sp. HKAR-2 and <10 % in Nostoc sp. HKAR-11 after 48 h of UV-B exposure. PAR had negligible negative impact on the survival of both cyanobacteria. The continuous exposure of UV-B and PAR?+?UV-B showed rapid uncoupling, bleaching, fragmentation, and degradation in both phycocyanin (C-PC) and phycoerythrin (C-PE) subunits of phycobiliproteins (PBPs). Remarkable bleaching effect of C-PE and C-PC was not only observed with UV-B or PAR?+?UV-B radiation, but longer period (24-48 h) of exposure with PAR alone also showed noticeable negative impact. The C-PE and C-PC subunits of the rice field isolate Nostoc sp. HKAR-11 were severely damaged in comparison to the hot spring isolate Nostoc sp. HKAR-2 with rapid wavelength shifting toward shorter wavelengths denoting the bleaching of both the accessory light harvesting pigments. The results indicate that PBPs of the hot spring isolate Nostoc sp. HKAR-2 were more stable under various light regimes in comparison to the rice field isolate Nostoc sp. HKAR-11 that could serve as a good source of valuable pigments to be used in various biomedical and biotechnological applications. PMID:25772678

  12. Base-catalyzed stereoselective vinylation of ketones with arylacetylenes: a new C(sp3)-C(sp2) bond-forming reaction.

    PubMed

    Trofimov, Boris A; Schmidt, Elena Yu; Ushakov, Igor' A; Zorina, Nadezhda V; Skital'tseva, Elena V; Protsuk, Nadezhda I; Mikhaleva, Al'bina I

    2010-07-26

    Alkylaryl- and alkylheteroarylketones, including those with condensed aromatic moieties, are readily vinylated with arylacetylenes (KOH/DMSO, 100 degrees C, 1 h) to give regio- and stereoselectively the (E)-beta-gamma-ethylenic ketones ((E)-3-buten-1-ones) in 61-84% yields and with approximately 100% stereoselectivity. This vinylation represents a new C(sp(3))-C(sp(2)) bond-forming reaction of high synthetic potential. PMID:20564291

  13. Binding and Agglutination of Streptococcus pneumoniae by Human Surfactant Protein D (SP-D) Vary between Strains, but SP-D Fails To Enhance Killing by Neutrophils

    PubMed Central

    Jounblat, Rania; Kadioglu, Aras; Iannelli, Francesco; Pozzi, Gianni; Eggleton, Paul; Andrew, Peter W.

    2004-01-01

    Recombinant human surfactant protein D (SP-D) expressed in Escherichia coli, consisting of the head and neck regions of the native molecule, bound to all strains of Streptococcus pneumoniae that were tested, but the extent of binding varied between strains of differing capsular serotypes. The recombinant protein expressed in the yeast Pichia pastoris did not bind. Full-length native SP-D aggregated pneumococci in a calcium-dependent manner that was inhibited by maltose acting as a competitive sugar. The ability of SP-D to modulate the uptake and killing of pneumococci by human neutrophils was also addressed. Neither recombinant truncated SP-D nor native full-length SP-D enhanced the killing of pneumococci by human neutrophils. Aggregation of pneumococci varied not only between strains of the same multilocus sequence type and different serotypes but also between strains of the same serotype. However, use of recombinant strains in which the serotype had been changed showed that the degree of aggregation was influenced by the capsular type. Indeed, a 19F serotype strain which was not aggregated by SP-D did exhibit aggregation when the original isogenic strain was capsule switched to capsular serotype 3. However, although our results show that SP-D is capable of aggregating most pneumococci, no correlation between the degree of aggregation and the capsule or multilocus sequence type of the pneumococcus was clearly apparent. Therefore, although the capsule serotype is not the only determinant of aggregation by SP-D, the data presented here indicate that it does have a role to play. PMID:14742512

  14. Cognitive-Behavioral Therapy for Suicide Prevention (CBT-SP): Treatment Model, Feasibility, and Acceptability

    ERIC Educational Resources Information Center

    Stanley, Barbara; Brown, Gregory; Brent, David A.; Wells, Karen; Poling, Kim; Curry, John; Kennard, Betsy D.; Wagner, Ann; Cwik, Mary F.; Klomek, Anat Brunstein; Goldstein, Tina; Vitiello, Benedetto; Barnett, Shannon; Daniel, Stephanie; Hughes, Jennifer

    2009-01-01

    Objective: To describe the elements of a manual-based cognitive-behavioral therapy for suicide prevention (CBT-SP) and to report its feasibility in preventing the recurrence of suicidal behavior in adolescents who have recently attempted suicide. Method: The CBT-SP was developed using a risk reduction and relapse prevention approach and…

  15. Lead biotransformation potential of allochthonous Bacillus sp. SKK11 with sesame oil cake in mine soil

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was aimed at assessing the potential of allochthonous Bacillus sp. SKK11 and sesame oil cake extract for transformation of Pb in mine soil. The bacteria were isolated from a brackish environment and identified as Bacillus sp. based on partial 16S rDNA sequences. The isolate SKK11 exhibite...

  16. Graphene-on-Diamond Devices with Increased Current-Carrying Capacity: Carbon sp2

    E-print Network

    Graphene-on-Diamond Devices with Increased Current-Carrying Capacity: Carbon sp2 -on-sp3 Technology with synthetic diamond, one can substantially increase the current-carrying capacity of graphene to as high as 18 on the single-crystal diamond substrates but also on an inexpensive ultrananocrystalline diamond, which can

  17. Parasitic isopods (Gnathia sp.) reduce haematocrit in captive blackeye thicklip (Labridae) on the Great

    E-print Network

    Grutter, Alexandra "Lexa"

    isopods are one of the most common ectoparasitic crustaceans on the Great Barrier Reef (GBR) (Grutter of juvenile Gnathia sp. have on teleost haematocrit, individual ectoparasite-free H. melapterus were exposed to one of two treatments: exposure to infection by Gnathia sp. or no exposure (control). The study took

  18. Metschnikowia proteae sp. nov., a nectarivorous insect-associated yeast species from Africa

    E-print Network

    Herrera, Carlos M.

    isolated from Heterochelus sp. represent two novel undescribed species (Candida sp. 1 EBDM2Y3 and Candida; Lachance et al., 2006; Rosa et al., 2007). The number of Candida and Metschnikowia species in the Metschnikowia clade has almost quadrupled in the last ten years with 39 Metsch- nikowia species and 26 Candida

  19. Genome sequence of Pantoea sp. strain Sc 1 an opportunistic cotton pathogen

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pantoea is comprised of a broad spectrum of species including plant pathogens. Here, we provide an annotated genome sequence of Pantoea sp. strain Sc 1, which was isolated from a diseased cotton boll. This research provides the first genome sequence of a bona fide Pantoea sp. insect vectored cotton...

  20. Draft Genome Sequence of Pedobacter sp. Strain Hv1, an Isolate from Medicinal Leech Mucosal Castings

    PubMed Central

    Ott, Brittany M.; Beka, Lidia; Graf, Joerg

    2015-01-01

    The Pedobacter sp. Hv1 strain was isolated from the medicinal leech, Hirudo verbana, mucosal castings. These mucosal sheds have been demonstrated to play a role in horizontal symbiont transmission. Here, we report the draft 4.9 Mbp genome sequence of Pedobacter sp. strain Hv1. PMID:26679583

  1. Disease development and genotypic diversity of Puccinia graminis f. sp. avenae in Swedish oat fields

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The disease development and population structure of Puccinia graminis f. sp. avenae, which causes stem rust on oat, were studied to investigate if sexual reproduction plays an important role in the epidemiology of the disease. The genetic population structure of P. graminis f. sp. avenae in Sweden w...

  2. Genome Sequence of the Plant Growth-Promoting Rhizobacterium Bacillus sp. Strain 916

    PubMed Central

    Luo, Chuping

    2012-01-01

    Bacillus sp. strain 916, isolated from the soil, showed strong activity against Rhizoctonia solani. Here, we present the high-quality draft genome sequence of Bacillus sp. strain 916. Its 3.9-Mb genome reveals a number of genes whose products are possibly involved in promotion of plant growth or antibiosis. PMID:22965091

  3. Draft Genome Sequence of the Streptothricin-Producing Strain Streptomyces sp. fd2-tb

    PubMed Central

    Tang, Biao; Yu, Yucong; Cen, Xufeng; Zhu, Yongqiang; Dai, Ruixue; Wang, Xianwei

    2015-01-01

    Streptomyces sp. fd2-tb can produce streptothricin class antibiotics with broad antimicrobial spectra. To better understand the mechanism of streptothricin biosynthesis and to assess the capacity of this strain in secondary metabolism, we report the draft genome sequence of Streptomyces sp. strain fd2-tb. PMID:26514767

  4. SP3X : a six-degree of freedom device for natural model creation

    E-print Network

    Whitney, Richard Henry, III

    2007-01-01

    This thesis presents a novel input device, called SP3X, for the creation of digital models in a semi-immersive environment. The goal of SP3X is to enable novice users to construct geometrically complex three-dimensional ...

  5. Fungal Diversity Ceratocystis manginecans sp. nov., causal agent of a destructive

    E-print Network

    Fungal Diversity 213 Ceratocystis manginecans sp. nov., causal agent of a destructive mango wilt). Ceratocystis manginecans sp. nov., causal agent of a destructive mango wilt disease in Oman and Pakistan. Fungal Diversity 27: 213-230. A serious wilt disease of mango trees (Mangifera indica) has recently

  6. Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus)

    PubMed Central

    Tate, Cynthia M.; Howerth, Elizabeth W.; Mead, Daniel G.; Dugan, Vivien G.; Luttrell, M. Page; Sahora, Alexandra I.; Munderloh, Ulrike G.; Davidson, William R.; Yabsley, Michael J.

    2014-01-01

    Recently, an undescribed Anaplasma sp. (also called Ehrlichia-like sp. or WTD agent) was isolated in ISE6 tick cells from captive white-tailed deer. The goal of the current study was to characterize this organism using a combination of experimental infection, morphologic, serologic, and molecular studies. Each of 6 experimentally inoculated white-tailed deer fawns (Odocoileus virginianus) became chronically infected (100+ days) with the Anaplasma sp. by inoculation of either infected whole blood or culture. None of the deer showed evidence of clinical disease, but 3 of the 6 deer evaluated had multiple episodes of transient thrombocytopenia. Light microscopy of Giemsa-stained, thin blood smears revealed tiny, dark, spherical structures in platelets of acutely infected deer. Anaplasma sp. was detected in platelets of inoculated deer by polymerase chain reaction, transmission electron microscopy, immunohistochemistry, and in situ hybridization. Five of 6 deer developed antibodies reactive to Anaplasma sp. antigen, as detected by indirect fluorescent antibody testing. Phylogenetic analyses of 16S rRNA, groESL, and gltA sequences confirmed the Anaplasma sp. is related to A. platys. Two attempts to transmit the Anaplasma sp. between deer by feeding Amblyomma americanum, a suspected tick vector, were unsuccessful. Based on its biologic, antigenic, and genetic characteristics, this organism is considered a novel species of Anaplasma, and the name Anaplasma odocoilei sp. nov. is proposed with UMUM76T (=CSUR-A1) as the type strain. PMID:23276749

  7. Sp1-CD147 positive feedback loop promotes the invasion ability of ovarian cancer.

    PubMed

    Zhao, Jing; Ye, Wei; Wu, Juan; Liu, Lijuan; Yang, Lina; Gao, Lu; Chen, Biliang; Zhang, Fanglin; Yang, Hong; Li, Yu

    2015-07-01

    CD147 is a novel cancer biomarker that has been confirmed to be overexpressed in ovarian carcinoma, which is significantly associated with poor prognosis. Although the Sp1 protein regulates the expression level of CD147, it remains unclear whether Sp1 phosphorylation plays a role in this regulation. A dual-luciferase assay revealed that T453 and T739 mutations decreased the activity of Sp1 binding to the promoter of CD147, followed by a decrease in CD147 mRNA and protein expression. Western blot analysis showed that CD147 promoted Sp1 phosphorylation at T453 and T739 through the PI3K/AKT and MAPK/ERK pathways. In addition, blocking the Sp1-CD147 positive feedback loop reduced the invasion ability of HO-8910pm cells. Immunohistochemical staining showed that the components of the feedback loop were overexpressed in ovarian cancer tissues. The correlation analysis revealed a significant correlation between phospho-Sp1 (T453), phospho-Sp1 (T739) and CD147 expression levels, with correlation coefficients of r=0.477 and r=0.461, respectively. Collectively, our results suggest that a Sp1-CD147 positive feedback loop plays a critical role in the invasion ability of ovarian cancer cells. PMID:25998266

  8. Locally semisimple and maximal subalgebras of the finitary Lie algebras gl(), sl(), so(), and sp()

    E-print Network

    Penkov, Ivan

    Locally semisimple and maximal subalgebras of the finitary Lie algebras gl(), sl(), so(), and sp. In the absence of Weyl's semisimplicity results for locally finite infinite­dimensional Lie algebras subalgebras of the finitary Lie algebras gl(), sl(), so(), and sp(). For simple finite­dimensional Lie

  9. 1 Estimating aquifer hydraulic properties from the inversion of surface 2 Streaming Potential (SP) anomalies

    E-print Network

    Sailhac, Pascal

    1 Estimating aquifer hydraulic properties from the inversion of surface 2 Streaming Potential (SP with the geometry of the water table. It follows that 11 SP measurements can be used to estimate aquifer hydraulic and found that we 14 are able to estimate the hydraulic conductivity and the depth 15 and the thickness

  10. LOVE, M. S., AND M. MOSER. 1976. Dauisia reginae sp. n. (Protozoa: Myxosporida) from

    E-print Network

    LOVE, M. S., AND M. MOSER. 1976. Dauisia reginae sp. n. (Protozoa: Myxosporida) from four, and Washington marine and estuarine fishes. U.s. Dep. Commer., NOAA Tech. Rep. NMFS SSRF-777, 576 p. LOVE, M. S. Soc. Spec. Pub!. 5. MOSER, M., AND M. S. LOVE. 1975. Henneguya sebasta sp. n. (Protozoa, Myxosporida

  11. Synergistic activation of a human promoter in vivo by transcription factor Sp1

    SciTech Connect

    Anderson, G.M. ); Freytag, S.O. )

    1991-04-01

    Many eucaryotic promoters contain multiple binding sites for sequence-specific DNA-binding proteins. In some cases, these proteins have been shown to interact synergistically to activate transcription. In this study, the authors address the possibility that the transcription factor Sp1 can synergistically activate a native human promoter in a cellular context that closely resembles that of a single-copy gene. Using DNase I footprinting with affinity-purified Sp1, they show that the human argininosuccinate synthetase (AS) promoter contains three sites that bind Sp1 with different affinities. These binding sites were mutated to abolish Sp1 binding, individually and in all possible combinations, to generate a series of AS promoter-CAT constructs was then measured in stably transfected human RPMI 2650 cells lines. The results show that each of the three Sp1-binding sites contributes to full activation of the human AS promoter and the relative contribution of each site correlates well with its in vitro affinity for Sp1. They provide direct evidence that Sp1-binding sites in their native context in a human promoter can interact synergistically in vivo to activate transcription. The ability to activate transcription synergistically may be the reason that many cellular promoters have multiple Sp1-binding sites arranged in tandem and in close proximity.

  12. LOCALIZATION OF THE SPERM PROTEIN SP22 AND INHIBITION OF FERTILITY IN VIVO AND IN VITRO

    EPA Science Inventory

    We previously established that the levels sperm membrane protein SP22 are highly correlated with the fertility of sperm from the cauda epididymidis of rats exposed to both epididymal and testicular toxicants, and that a testis-specific SP22 transcript is expressed in post-meiotic...

  13. Sp1 transcriptionally regulates BRK1 expression in non-small cell lung cancer cells.

    PubMed

    Li, Meng; Ling, Bing; Xiao, Ting; Tan, Jinjing; An, Ning; Han, Naijun; Guo, Suping; Cheng, Shujun; Zhang, Kaitai

    2014-06-01

    Following a previous study reporting that BRK1 is upregulated in non-small cell lung cancer (NSCLC), the present study sought to clarify the role of specificity protein 1 (Sp1) in the transcriptional regulation of the BRK1 gene. Therefore, a construct, named F8, consisting of the -1341 to -1 nt sequence upstream of the start codon of the BRK1 gene inserted into pGL4.26 was made. A series of truncated fragments was then constructed based on F8. Segment S831, which contained the -84 to -1 nt region, displayed the highest transcriptional activity in the A549, H1299 and H520 NSCLC cell lines. Bioinformatic analysis showed a potential Sp1-binding element at -73 to -64 nt, and a mutation in this region suppressed the transcriptional activity of S831. Then the RNAi assays of Sp1 and its coworkers Sp3 and Sp4 were performed, and suppression of Sp1 by siRNA inhibited the mRNA expression of BRK1. Both an electrophoretic mobility shift assay (EMSA) and a chromatin immunoprecipitation (ChIP) assay demonstrated that Sp1 bound to the promoter area of the BRK1 gene. Our data identified a functional and positive Sp1 regulatory element from -73 to -64 nt in the BRK1 promoter, which may likely explain the overexpression of BRK1 in NSCLC. PMID:24680773

  14. Alishewanella agri sp. nov., isolated from landfill soil Min-Soo Kim,1,2

    E-print Network

    Bae, Jin-Woo

    Alishewanella agri sp. nov., isolated from landfill soil Min-Soo Kim,1,2 3 Seon Kyung Jo,3 3 Seong, named Alishewanella agri sp. nov. The genus Alishewanella was first proposed by Vogel et al. (2000, Alishewanella jeotgali MS1T was isolated from a traditional fermented food in Korea by Kim et al. (2009

  15. First identification of Trichinella sp. in golden jackal (Canis aureus) in Romania.

    PubMed

    Blaga, R; Gherman, C; Seucom, D; Cozma, V; Boireau, P

    2008-04-01

    Larvae of Trichinella sp. were identified in a golden jackal (Canis aureus) from Romania by both trichinelloscopy and artificial digestion. The larvae were identified as Trichinella britovi using a multiplex polymerase chain reaction biotyping method. This is the first report of Trichinella sp. in a jackal in Romania. PMID:18436679

  16. Effect of Cys85 on biochemical properties and biological function of human SP-A variants

    PubMed Central

    Wang, Guirong; Myers, Catherine; Mikerov, Anatoly; Floros, Joanna

    2008-01-01

    Four “core” amino acid differences within the collagen-like domain distinguish the human surfactant proteins A1 (SP-A1) variants from the SP-A2 variants. One of these, cysteine 85 that could form intermolecular disulfide bonds, is present in SP-A1 (Cys85) and absent in SP-A2 (Arg85). We hypothesized that residue85 affects both structure and function of SP-A1 and SP-A2 variants. To test this, wild type (WT) variants, 6A2 of SP-A1 and 1A0 of SP-A2, and their mutants (6A2(C85R) and 1A0(R85C)), were generated and studied. We found: 1) Residue85 affected the binding ability to mannose and the oligomerization pattern of SP-As. The 1A0(R85C) and 6A2(C85R) patterns were similar and/or resembled those of WT 6A2 and 1A0, respectively. 2) Both SP-A WT and mutants differentially induced rough LPS and Pseudomonas aeruginosa aggregation in the following order: 1A0 > 6A2 > 6A2(C85R) > 1A0(R85C) for Re-LPS aggregation, and 1A0 > 6A2 = 6A2(C85R) = 1A0(R85C) for bacterial aggregation. 3) SP-A WT and mutants enhanced phagocytosis of P. aeruginosa by rat alveolar macrophages. Their phagocytic index order was: 6A2(C85R) > 1A0 > 6A2 = 1A0(R85C). The activity of mutant 1A0(C85R) was significantly lower from WT 1A0 but similar to 6A2. Compared to WT 6A2, the 6A2(C85R) mutant exhibited a significantly higher activity. These results indicate that SP-A variant/mutant with Arg85 exhibits higher ability to enhance bacterial phagocytosis than that with Cys85. Residue85 plays a important role in the structure and function of SP-A, and is a major factor for the differences between SP-A1 and SPA2 variants. PMID:17580966

  17. Correlation of the Genetic Map and the Endonuclease Site Map of Bacillus subtilis Bacteriophage SP02

    PubMed Central

    Graham, S.; Sutton, S.; Yoneda, Y.; Young, F. E.

    1982-01-01

    By marker rescue of bacteriophage SP02 sus mutants with purified bacteriophage SP02 DNA fragments, 11 of the 17 known bacteriophage SP02 sus loci were assigned to discrete DNA fragments. The left-most genetic locus, susA, was found to reside near one bacteriophage SP02 terminus (EcoRI-C1 fragment), whereas the right-most genetic locus, susP, was found to reside near the other bacteriophage SP02 terminus (EcoRI-C2 fragment). The physical locations of the intervening genetic loci were found to be consistent with the previously determined genetic order. Evidence was also obtained which suggested that at least one end of a transforming DNA fragment is degraded during DNA uptake by the competent bacterium. PMID:16789220

  18. Process optimization for the extraction of polyphenol from Malaysian brown seaweed, Sargassum sp.

    NASA Astrophysics Data System (ADS)

    Ismail, Norra; Abdullah, Aminah

    2015-09-01

    The optimum extraction parameters of Malaysian brown seaweed, Sargassum sp. were determined by using Response Surface Methodology (RSM). Central Composite Face-centered Design (CCFD) was used to study the influence of extraction temperature (40-60°C), extraction time (60-180 minute) and liquid to solid ratio (20-40 mL/g) on total phenolic content (TPC) of Sargassum sp. dried extracts. The statistical analysis of the experiment indicated that extraction temperatue and time had significant effect on Sargassum sp. total phenolic contents (TPC). The CCFD showed that quadratic regression models were in good agreement with the experimental results with the coefficients of determination (R2) of 0.8066 for Sargassum sp. TPC. The extraction temperature of 40°C, extraction time of 180 minute and liquid-solid ratio of 40 mL/g were the most efficient for the extraction of polyphenols from Sargassum sp. dried powder.

  19. Aeromonas fluvialis sp. nov., isolated from a Spanish river.

    PubMed

    Alperi, Anabel; Martínez-Murcia, Antonio J; Monera, Arturo; Saavedra, Maria J; Figueras, Maria J

    2010-01-01

    A Gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(T), was isolated from a water sample collected from the Muga river, Girona, north-east Spain. Preliminary analysis of the 16S rRNA gene sequence showed that this strain belonged to the genus Aeromonas, the nearest species being Aeromonas veronii (99.5 % similarity, with seven different nucleotides). A polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) showed isolate 717(T) to be an independent phylogenetic line, with Aeromonas sobria, Aeromonas veronii and Aeromonas allosaccharophila as the closest neighbour species. DNA-DNA reassociation experiments and phenotypic analysis identified that strain 717(T) represents a novel species, for which the name Aeromonas fluvialis sp. nov. is proposed, with type strain 717(T) (=CECT 7401(T) =LMG 24681(T)). PMID:19648345

  20. Complete genome sequence of Arthrobacter sp. strain FB24

    SciTech Connect

    Nakatsu, C. H.; Barabote, Ravi; Thompson, Sue; Bruce, David; Detter, Chris; Brettin, T.; Han, Cliff F.; Beasley, Federico; Chen, Weimin; Konopka, Allan; Xie, Gary

    2013-09-30

    Arthrobacter sp. strain FB24 is a species in the genus Arthrobacter Conn and Dimmick 1947, in the family Micrococcaceae and class Actinobacteria. A number of Arthrobacter genome sequences have been completed because of their important role in soil, especially bioremediation. This isolate is of special interest because it is tolerant to multiple metals and it is extremely resistant to elevated concentrations of chromate. The genome consists of a 4,698,945 bp circular chromosome and three plasmids (96,488, 115,507, and 159,536 bp, a total of 5,070,478 bp), coding 4,536 proteins of which 1,257 are without known function. This genome was sequenced as part of the DOE Joint Genome Institute Program.

  1. JSD: Parallel Job Accounting on the IBM SP2

    NASA Technical Reports Server (NTRS)

    Saphir, William; Jones, James Patton; Walter, Howard (Technical Monitor)

    1995-01-01

    The IBM SP2 is one of the most promising parallel computers for scientific supercomputing - it is fast and usually reliable. One of its biggest problems is a lack of robust and comprehensive system software. Among other things, this software allows a collection of Unix processes to be treated as a single parallel application. It does not, however, provide accounting for parallel jobs other than what is provided by AIX for the individual process components. Without parallel job accounting, it is not possible to monitor system use, measure the effectiveness of system administration strategies, or identify system bottlenecks. To address this problem, we have written jsd, a daemon that collects accounting data for parallel jobs. jsd records information in a format that is easily machine- and human-readable, allowing us to extract the most important accounting information with very little effort. jsd also notifies system administrators in certain cases of system failure.

  2. Novel sp3-hybridized framework structure of group 14 elements

    NASA Astrophysics Data System (ADS)

    Nguyen, Manh Cuong; Zhao, Xin; Wang, Cai-Zhuang; Ho, Kai-Ming

    2014-03-01

    Using genetic algorithm atomic structure prediction method and first-principles calculations, we discovered a novel low-energy metastable structure of group 14 elements in P42/mnm symmetry. The P42/mnm structure is a cage-like distorted sp3-hybridized framework structure with the cage's volume ~ 4% larger than the average cage's volume of the clathrate type-I structure, indicating P42/mnm structure a good gases or metal atoms encapsulation structure. The band structure calculations show that P42/mnm Si and Ge are semiconducting with energy band gaps close to the optimal values for optoelectronic or photovoltaic applications. The metal atom encapsulation P42/mnm structure of group 14 elements could also be a candidate for rattling-mediated superconducting or ``a phonon glass and an electrical crystal'' thermoelectric materials.

  3. Coniosporium epidermidis sp. nov., a new species from human skin

    PubMed Central

    Li, D. M.; de Hoog, G.S.; Saunte, D.M. Lindhardt; van den Ende, A.H.G. Gerrits; Chen, X. R.

    2008-01-01

    Coniosporium epidermidis sp. nov. is described from a superficial skin lesion with blackish discolouration in an 80-yr-old Chinese patient. The species produces dark, thick-walled, inflated, reluctantly liberating arthroconidia without longitudinal septa. Sequences of the ribosomal operon, as well as of the translation elongation factor 1-? support its novelty. The species is found in a lineage basal to the order Chaetothyriales, amidst relatives from rock, but also species repeatedly isolated from human skin and nails and eventually causing mild cutaneous infections. Coniosporium epidermidis is consistently found on humans, either asymptomatic or symptomatic. The species indicates a change of life style towards human pathogenicity, which is a recurrent type of ecology in derived Chaetothyriales. Superficial and cutaneous infection by melanized fungi is a new category in dermatology. PMID:19287535

  4. Release of polysaccharide by sonication of cells ( Porphyridium sp.)

    NASA Astrophysics Data System (ADS)

    Faerman, V.; Mukmenev, I.; Shreiber, I.

    2009-03-01

    The paper deals with experimental data concerning the interaction of acoustical waves with microbial cell (Porphyridium sp.). The aim of the present paper was to increase the amount of biopolymer released from the microorganisms biomass with the aid ultrasound irradiation without scission or a decrease in the molecular weight. The results indicated that the amount of polysaccharide (for example) released from the cell pellet could be enhanced by ultrasound, depending on the frequency and energy of the ultrasound. The sugar composition remain the same, but the apparent viscosity of polysaccharide aqueous solutions decreased, indicated that some changes in the molecular shape and size occurred. When ultrasound irradiation was applied in the presence of either CO2 or CO2 + H2, the apparent viscosity of polysaccharide aqueous solutions increased (versus usual ultrasound treatment).

  5. Study of the resistance of Acinetobacter sp. to mercuric chloride

    SciTech Connect

    Lomovskaya, O.L.; Mindlin, S.Z.; Khesin, R.B.

    1986-06-01

    In addition to large plasmids (approx 60 kb) a small plasmid (almost 7.5 kb), plasmid PKL1, has been found in HgCl/sub 2/-resistant strains of Acinetobacter sp. isolated from soil in the vicinity of the Khaidarkan mercury deposit. With the aid of conjugation and transformation studies it was established that plasmid pKL1 is a mobilized plasmid with a broad host range and that this plasmid carries the Hg/sup r/-determinant. A restriction map of plasmid pKL1 was constructed, and the site of the Hg/sup r/-determinant and the regions essential for replication were localized. By comparing the results of the present study and previously-obtained data it was proposed that in a given microbiocoenosis the Hg/sup r/-determinants may occur in plasmids which differ markedly in structure and properties.

  6. Power plant system assessment. Final report. SP-100 Program

    SciTech Connect

    Anderson, R.V.; Atkins, D.F.; Bost, D.S.; Berman, B.; Clinger, D.A.; Determan, W.R.; Drucker, G.S.; Glasgow, L.E.; Hartung, J.A.; Harty, R.B.

    1983-10-31

    The purpose of this assessment was to provide system-level insights into 100-kWe-class space reactor electric systems. Using these insights, Rockwell was to select and perform conceptual design studies on a ''most attractive'' system that met the preliminary design goals and requirements of the SP-100 Program. About 4 of the 6 months were used in the selection process. The remaining 2 months were used for the system conceptual design studies. Rockwell completed these studies at the end of FY 1983. This report summarizes the results of the power plant system assessment and describes our choice for the most attractive system - the Rockwell SR-100G System (Space Reactor, 100 kWe, Growth) - a lithium-cooled UN-fueled fast reactor/Brayton turboelectric converter system.

  7. [A novel trichostatin analogue culture of Streptomyces sp. CPCC 203909].

    PubMed

    Chen, Ming-hua; Wu, Ye-xiang; Xu, Yan-ni; Yu, Li-yan; Hong, Bin; Jiang, Wei; Si, Shu-yi

    2015-05-01

    By using a cell-based high throughput screening model for the CLA-1 up-regulator, Streptomyces 203909 was found to produce up-regulator of CLA-1. A novel trichostatin analogue was isolated from the rice fermentation of Streptomyces sp. CPCC 203909by a combination of various chromatographic techniques including column chromatography (CC) over silica gel, flash C18 CC, and reversed-phase HPLC. Its structure was identified as (-)-(R,2E,4Z)-7-[(4'-dimethylamino) phenyl]-4,6-dimethyl-7-oxohepta-2,4-dienoyl-L-glutamine (1) by the spectroscopic and chemical methods, and combination with the CD spectroscopy and Marfey's method. In the prelimi- nary assays, Compound 1 showed cytotoxicity against human embryonic kidney 293 cell line with IC50 value 35.3 [µmol · L(-1). PMID:26323142

  8. Saccharothrix violaceirubra sp. nov., isolated from soil and plant litter.

    PubMed

    Otoguro, Misa; Tamura, Tomohiko; Suzuki, Ken-Ichiro; Hayakawa, Masayuki

    2009-06-01

    Two strains of nocardioform actinomycetes, isolated from soil and plant litter in Yamanashi prefecture, Japan, showed substrate mycelium with purple to dark-red colours. 16S rRNA gene sequence analysis indicated that the organisms belonged to the family Actinosynnemataceae and were related closely to Saccharothrix strains (96.7-98.0 % sequence similarity). The isolates contained MK-9(H(4)) as the predominant menaquinone, meso-diaminopimelic acid, galactose as the diagnostic whole-cell sugar and phosphatidylethanolamine as the predominant polar lipid. Based on a combination of cultural, physiological and chemotaxonomic properties, in addition to the phylogenetic analysis and DNA-DNA hybridization data, we propose a novel species, Saccharothrix violaceirubra sp. nov., for these strains, with the type strain YU 692-1(T) (=NBRC 102064(T) =KCTC 19326(T)). PMID:19502343

  9. Diverse sp3 C-H functionalization through alcohol ?-sulfonyloxylation

    NASA Astrophysics Data System (ADS)

    Xu, Yan; Yan, Guobing; Ren, Zhi; Dong, Guangbin

    2015-10-01

    Site-selective C-H functionalization has emerged as an attractive tool for derivatizing complex synthetic intermediates, but its use for late-stage diversification is limited by the functional groups that can be introduced, especially at unactivated sp3-hybridized positions. To overcome this, we introduce a strategy that directly installs a sulfonyloxy group at a ?-C-H bond of a masked alcohol and subsequently employs nucleophilic substitution reactions to prepare various derivatives. Hydroxyl groups are widely found in bioactive molecules and are thus readily available as synthetic handles. A directing group is easily added (and subsequently removed) from the alcohols such that a formal site-selective ?-C-H sulfonyloxylation of these alcohols is achieved. Substitution reactions with carbon, nitrogen, oxygen and other nucleophiles then lead to diverse functionalizations that may help to streamline the synthesis of complex analogues for drug discovery.

  10. Life Cycle Reversal in Aurelia sp.1 (Cnidaria, Scyphozoa)

    PubMed Central

    He, Jinru; Zheng, Lianming; Zhang, Wenjing; Lin, Yuanshao

    2015-01-01

    The genus Aurelia is one of the major contributors to jellyfish blooms in coastal waters, possibly due in part to hydroclimatic and anthropogenic causes, as well as their highly adaptive reproductive traits. Despite the wide plasticity of cnidarian life cycles, especially those recognized in certain Hydroza species, the known modifications of Aurelia life history were mostly restricted to its polyp stage. In this study, we document the formation of polyps directly from the ectoderm of degenerating juvenile medusae, cell masses from medusa tissue fragments, and subumbrella of living medusae. This is the first evidence for back-transformation of sexually mature medusae into polyps in Aurelia sp.1. The resulting reconstruction of the schematic life cycle of Aurelia reveals the underestimated potential of life cycle reversal in scyphozoan medusae, with possible implications for biological and ecological studies. PMID:26690755

  11. Study on bioactive compounds from Streptomyces sp. ANU 6277.

    PubMed

    Narayana, Kolla J P; Prabhakar, Peddikotla; Vijayalakshmi, Muvva; Venkateswarlu, Yenamandra; Krishna, Palakodety S J

    2008-01-01

    An attempt was made to study the bioactive compounds from a terrestrial Streptomyces sp. ANU 6277 isolated from laterite soil. Four active fractions were recovered from the solvent extracts obtained from the culture broth of five day-old strain. Three bioactive compounds were purified and identified as 3-phenylpropionic acid, anthracene-9,10-quinone and 8-hydroxyquinoline. The components of the partially purified fourth active fraction were analyzed by gas chromatography-mass spectrometry and identified as benzyl alcohol, phenylethyl alcohol and 2H-1, 4-benzoxazin-3 (4H)-one. Four active fractions were screened for antimicrobial activity against Gram-positive and Gram-negative bacteria, and fungi including phytopathogenic, toxigenic and dermatophytic genera. Among these metabolites, 8-hydroxyquinoline exhibited strong antibacterial and antifungal activity as compared to 3-phenylpropionic acid and anthracene-9,10-quinone. PMID:18610654

  12. Failure Analysis of Nonvolatile Residue (NVR) Analyzer Model SP-1000

    NASA Technical Reports Server (NTRS)

    Potter, Joseph C.

    2011-01-01

    National Aeronautics and Space Administration (NASA) subcontractor Wiltech contacted the NASA Electrical Lab (NE-L) and requested a failure analysis of a Solvent Purity Meter; model SP-IOOO produced by the VerTis Instrument Company. The meter, used to measure the contaminate in a solvent to determine the relative contamination on spacecraft flight hardware and ground servicing equipment, had been inoperable and in storage for an unknown amount of time. NE-L was asked to troubleshoot the unit and make a determination on what may be required to make the unit operational. Through the use of general troubleshooting processes and the review of a unit in service at the time of analysis, the unit was found to be repairable but would need the replacement of multiple components.

  13. Identification of Oxaphenalenone Ketals from the Ascomycete Fungus Neonectria sp.

    PubMed

    Ren, Jinwei; Niu, Shubing; Li, Li; Geng, Zhufeng; Liu, Xingzhong; Che, Yongsheng

    2015-06-26

    Neonectrolides B-E (4-7), four new oxaphenalenone ketals incorporating the new furo[2,3-b]isochromeno[3,4,5-def]chromen-11(6aH)-one skeleton, were isolated from the fermentation extract of the ascomycete fungus Neonectria sp. in an in-depth investigation guided by HPLC fingerprint and a cytotoxicity assay. The previously identified oxaphenalenone spiroketal neonectrolide A (1) and its putative biosynthetic precursors (2 and 3) were also reisolated in the current work. The structures of 4-7 were primarily elucidated by interpretation of NMR spectroscopic data, and the absolute configurations were deduced by electronic circular dichroism calculations. Compound 6 showed cytotoxic effects against four of the six human tumor cell lines tested. Biosynthetically, compounds 4-7 could be derived via the Diels-Alder reaction cascades starting from derivatives of the co-isolated metabolites 2 and 3. PMID:25978132

  14. Purification and characterization of an alkaline keratinase from Streptomyces sp.

    PubMed

    Tatineni, Radhika; Doddapaneni, Kiran Kumar; Potumarthi, Ravi Chandra; Vellanki, Ravi Nagaraj; Kandathil, Manjusha Thomas; Kolli, Nilima; Mangamoori, Lakshmi Narasu

    2008-04-01

    A protease producing bacterial culture ('S7') was isolated from slaughterhouse waste samples, Hyderabad, India. It was related to Streptomyces sp. on the basis of biochemical properties and 16S rRNA gene sequencing. Purification of the protease present in the culture medium supernatant on sephacryl S-100 indicated that it contains a keratinase with 67% recovery, 2.5-fold purification and an estimated molecular mass of approximately 44,000 Da. Keratinase showed an optimal activity at 45 degrees C and pH 11. Keratinase activity increased substantially in presence of Ca(2+) and was inhibited in presence of PMSF and EDTA identifying it as a serine metalloprotease. Stability in the presence of detergents, surfactants and solvents make this keratinase extremely useful for biotechnological process involving keratin hydrolysis or in the leather industry. PMID:17804219

  15. Six Cases of Verminous Pneumonia (Muellerius Sp.) in Goats

    PubMed Central

    Nimmo, Judith S.

    1979-01-01

    Six cases of verminous pneumonia in goats due to Muellerius sp. were reviewed. In only one case was the pneumonia diagnosed antemortem. The gross necropsy findings and histopathology revealed a widespread interstitial pneumonia in all the cases. There was variability in the local reaction around the parasites from almost none in the mildest cases to larger focal accumulations of macrophages and mononuclear inflammatory cells. Only in the severest cases were eosinophils seen and even then were scattered and few in number. No nodular lesions were seen associated with the parasites. The pathology of the pulmonary lesions in goats appears to be more commonly of the diffuse type and therefore significantly different from the nodular lesion usually observed in sheep. ImagesFIGURE 1.FIGURE 2.FIGURE 3.FIGURE 4. PMID:436107

  16. Preparation of immobilized microorganism (B. SP 3012) by ?-irradiation

    NASA Astrophysics Data System (ADS)

    Tung-Fu, Lin

    Gel entrapment using ?-ray irradiation provides an efficient method to prepare immobilized fumarase-producing microorganism (B.SP 3012). In the use of such immobilized microorganism for producing L-malic acid from fumaric acid, a high value of the activity of fumarase (13000 ?m/h/g) has been obtained. The enzymatic reaction has been carried out at pH = 6.5 and 37°C. In the case of the column method optimum reaction condition for conversion of fumaric acid to L-malic acid can be easily obtained by adjusting the space velocity of the substrate solution. Due to the sufficient durability of enzymatic activity, the immobilized microorganism can be used repeatedly.

  17. Spacecraft and mission design for the SP-100 flight experiment

    NASA Technical Reports Server (NTRS)

    Deininger, William D.; Vondra, Robert J.

    1988-01-01

    The design and performance of a spacecraft employing arcjet nuclear electric propulsion, suitable for use in the SP-100 Space Reactor Power System (SRPS) Flight Experiment, are outlined. The vehicle design is based on a 93 kW(e) ammonia arcjet system operating at an experimentally measured specific impulse of 1031 s and an efficiency of 42.3 percent. The arcjet/gimbal assemblies, power conditioning subsystem, propellant feed system, propulsion system thermal control, spacecraft diagnostic instrumentation, and the telemetry requirements are described. A 100 kW(e) SRPS is assumed. The spacecraft mass is baselined at 5675 kg excluding the propellant and propellant feed system. Four mission scenarios are described which are capable of demonstrating the full capability of the SRPS. The missions considered include spacecraft deployment to possible surveillance platform orbits, a spacecraft storage mission, and an orbit raising round trip corresponding to possible orbit transfer vehicle (OTV) missions.

  18. Alkaloids from an algicolous strain of Talaromyces sp.

    NASA Astrophysics Data System (ADS)

    Yang, Haibin; Li, Fang; Ji, Naiyun

    2015-09-01

    Compounds isolated and identified in a culture of the alga-endophytic fungus Talaromyces sp. cf-16 included two naturally occurring alkaloids, 2-[(S)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (1a) and 2-[(R)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (1b), that were identified for the first time. In addition, seven known compounds (2-8) were obtained from the culture. Following chiral column chromatography, compounds 1a and 1b were identified as enantiomers by spectroscopic analyses and quantum chemical calculations. Bioassay results showed that 5 was more toxic to brine shrimp than the other compounds, and that 3-6 could inhibit Staphylococcus aureus.

  19. Complete genome sequence of Arthrobacter sp. strain FB24

    PubMed Central

    Nakatsu, Cindy H.; Barabote, Ravi; Thompson, Sue; Bruce, David; Detter, Chris; Brettin, Thomas; Han, Cliff; Beasley, Federico; Chen, Weimin; Konopka, Allan; Xie, Gary

    2013-01-01

    Arthrobacter sp. strain FB24 is a species in the genus Arthrobacter Conn and Dimmick 1947, in the family Micrococcaceae and class Actinobacteria. A number of Arthrobacter genome sequences have been completed because of their important role in soil, especially bioremediation. This isolate is of special interest because it is tolerant to multiple metals and it is extremely resistant to elevated concentrations of chromate. The genome consists of a 4,698,945 bp circular chromosome and three plasmids (96,488, 115,507, and 159,536 bp, a total of 5,070,478 bp), coding 4,536 proteins of which 1,257 are without known function. This genome was sequenced as part of the DOE Joint Genome Institute Program. PMID:24501649

  20. Desulfurization of dibenzothiophene by Corynebacterium sp. strain SY1

    SciTech Connect

    Omori, Toshio; Monna, L.; Saiki, Yuko; Kodama, Tohru )

    1992-03-01

    Strain SY1, identified as a Corynebacterium sp., was isolated on the basis of the ability to utilize dibenzothiophene (DBT) as a sole source of sulfur. Strain SY1 could utilize a wide range of organic and inorganic sulfur compounds, such as DBT sulfone, dimethyl sulfide, dimethyl sulfoxide, dimethyl sulfone, CS{sub 2}, FeS{sub 2}, and even elemental sulfur. Strain SY1 metabolized DBT to dibenzothiophene-5-oxide, DBT sulfone, and 2-hydroxybiphenyl, which was subsequently nitrated to produce at least two different hydroxynitrobiphenyls during cultivation. These metabolites were separated by silica gel column chromatography and identified by nuclear magnetic resonance, UV, and mass spectral techniques. Resting cells of SY1 desulfurized toluenesulfonic acid and released sulfite anion. On the basis of these results, a new DBT degradation pathway is proposed.

  1. Antagonistic Effect of Streptomyces sp. BS062 against Botrytis Diseases.

    PubMed

    Kim, Young-Sook; Lee, In-Kyoung; Yun, Bong-Sik

    2015-09-01

    The use of microorganisms and their secreted molecules to prevent plant diseases is considered an attractive alternative and way to supplement synthetic fungicides for the management of plant diseases. Strain BS062 was selected based on its ability to inhibit the mycelial growth of Botrytis cinerea, a major causal fungus of postharvest root rot of ginseng and strawberry gray mold disease. Strain BS062 was found to be closely related to Streptomyces hygroscopicus (99% similarity) on the basis of 16S ribosomal DNA sequence analysis. Postharvest root rot of ginseng and strawberry gray mold disease caused by B. cinerea were controlled up to 73.9% and 58%, respectively, upon treatment with culture broth of Streptomyces sp. BS062. These results suggest that strain BS062 may be a potential agent for controlling ginseng postharvest root rot and strawberry gray mold disease. PMID:26539052

  2. Lipopeptides from the Tropical Marine Cyanobacterium Symploca sp.

    PubMed Central

    2015-01-01

    A collection of the tropical marine cyanobacterium Symploca sp., collected near Kimbe Bay, Papua New Guinea, previously yielded several new metabolites including kimbeamides A–C, kimbelactone A, and tasihalide C. Investigations into a more polar cytotoxic fraction yielded three new lipopeptides, tasiamides C–E (1–3). The planar structures were deduced by 2D NMR spectroscopy and tandem mass spectrometry, and their absolute configurations were determined by a combination of Marfey’s and chiral-phase GC-MS analysis. These new metabolites are similar to several previously isolated compounds, including tasiamide (4), grassystatins (5, 6), and symplocin A, all of which were isolated from similar filamentous marine cyanobacteria. PMID:24588245

  3. Induction of Gametogenesis in the Cnidarian Endosymbiosis Model Aiptasia sp.

    PubMed Central

    Grawunder, Désirée; Hambleton, Elizabeth A.; Bucher, Madeline; Wolfowicz, Iliona; Bechtoldt, Natascha; Guse, Annika

    2015-01-01

    Endosymbiosis is widespread among cnidarians and is of high ecological relevance. The tropical sea anemone Aiptasia sp. is a laboratory model system for endosymbiosis between reef-building corals and photosynthetic dinoflagellate algae of the genus Symbiodinium. Here we identify the key environmental cues to induce reproducible spawning in Aiptasia under controlled laboratory conditions. We find that simulating a lunar cycle with blue-wavelength light is necessary to promote abundant gamete production and synchronous release in well-fed animals. Sexual reproduction rates are genetically determined and differ among clonal lines under similar conditions. We also find the inverse difference in rates of asexual reproduction. This study provides the requisite basis for further development of the Aiptasia model system, allowing analysis of basic cellular and molecular mechanisms in the laboratory as well as investigations of broad questions of ecological and evolutionary relevance. PMID:26498008

  4. Antagonistic Effect of Streptomyces sp. BS062 against Botrytis Diseases

    PubMed Central

    Kim, Young-Sook; Lee, In-Kyoung

    2015-01-01

    The use of microorganisms and their secreted molecules to prevent plant diseases is considered an attractive alternative and way to supplement synthetic fungicides for the management of plant diseases. Strain BS062 was selected based on its ability to inhibit the mycelial growth of Botrytis cinerea, a major causal fungus of postharvest root rot of ginseng and strawberry gray mold disease. Strain BS062 was found to be closely related to Streptomyces hygroscopicus (99% similarity) on the basis of 16S ribosomal DNA sequence analysis. Postharvest root rot of ginseng and strawberry gray mold disease caused by B. cinerea were controlled up to 73.9% and 58%, respectively, upon treatment with culture broth of Streptomyces sp. BS062. These results suggest that strain BS062 may be a potential agent for controlling ginseng postharvest root rot and strawberry gray mold disease. PMID:26539052

  5. Bioactive isocoumarins from a terrestrial Streptomyces sp. ANK302.

    PubMed

    Zinad, Dhafer Saber; Shaaban, Khaled A; Abdalla, Muna Ali; Islam, Md Tofazzal; Schüffler, Anja; Laatsch, Hartmut

    2011-01-01

    Four isocoumarins have been isolated from the terrestrial Streptomyces sp. ANK302, namely 6,8-dimethoxy-3-methylisocoumarin (1), 6,8-dihydroxy-3-methylisocoumarin (2), 6,8-dihydroxy-7-methoxy-3-methylisocoumarin (3), and 6,7,8-trimethoxy-3-methylisocoumarin (4). Compound 1 is a new naturally-occurring isocoumarin, and 2 was isolated as a new bacterial product. The structures 1-4 were deduced from high resolution mass, 1D and 2D NMR spectra and by comparison with related compounds from the literature. Compound 2 showed a strong zoosporicidal activity at a concentration of 5 microg/mL against a phytopathogenic oomycete, Plasmopara viticola, and 1 was active against PMID:21366043

  6. Bioactive Compounds from a Gorgonian Coral Echinomuricea sp. (Plexauridae)

    PubMed Central

    Chung, Hsu-Ming; Hong, Pei-Han; Su, Jui-Hsin; Hwang, Tsong-Long; Lu, Mei-Chin; Fang, Lee-Shing; Wu, Yang-Chang; Li, Jan-Jung; Chen, Jih-Jung; Wang, Wei-Hsien; Sung, Ping-Jyun

    2012-01-01

    A new labdane-type diterpenoid, echinolabdane A (1), and a new sterol, 6-epi-yonarasterol B (2), were isolated from a gorgonian coral identified as Echinomuricea sp. The structures of metabolites 1 and 2 were elucidated by spectroscopic methods. Echinolabdane A (1) possesses a novel tetracyclic skeleton with an oxepane ring jointed to an ?,?-unsaturated-?-lactone ring by a hemiketal moiety, and this compound is the first labdane-type diterpenoid to be obtained from marine organisms belonging to the phylum Cnidaria. 6-epi-Yonarasterol B (2) is the first steroid derivative to be isolated from gorgonian coral belonging to the genus Echinomuricea, and this compound displayed significant inhibitory effects on the generation of superoxide anions and the release of elastase by human neutrophils. PMID:22822364

  7. PAH bioconcentration in Mytilus sp from Sinclair Inlet, WA

    SciTech Connect

    Frazier, J.; Young, D.; Ozretich, R.; Echols, S.

    1995-12-31

    Approximately 20 polynuclear aromatic hydrocarbons (PAH) were measured by GC/MS in seawater and whole soft tissues of the intertidal mussel Mytilus sp. collected in July 1991 within and around Puget Sound`s Sinclair Inlet. Low variability was observed in the water concentrations collected over three days at control sites, yielding reliable values for the exposure levels experienced by this bioindicator mollusk. Mean water concentrations of acenaphthene, phenanthrene, and fluoranthene in the control region were 2.7 {+-} 0.8, 2.8 {+-} 0.8, and 3.1 {+-} 0.7 ng/liter, respectively. Levels measured near sites of vessel activity were higher but much more variable; this reduced the reliability of the tissue/water bioconcentration factors (BCF) obtained from these samples. An empirical model relating values of Log BCF and Log Kow for the control zone samples supports the utility of this estuarine bioindicator for monitoring general levels of PAH in nearshore surface waters.

  8. The explicit representation for the angular flux solution in the simplified P{sub N} (SP{sub N}) theory

    SciTech Connect

    Chao, Y. A.; Yamamoto, A.

    2012-07-01

    The current SP{sub n} theory formulation, via either the asymptotic method or the variational method, does not provide an explicit and calculable representation for the corresponding angular flux solution. It is therefore not possible to reconstruct from the SP{sub n} solution the corresponding angular flux solution, or to extract from a reference transport solution the corresponding SP{sub n} solution. This makes it impossible to calculate the necessary surface discontinuity factors to force consistency between the SP{sub n} solution and the higher level transport solution. Without discontinuity factors, the superiority of SP{sub n} over diffusion could be significantly degraded in practical applications. In this paper we present a different SP{sub n} formulation that provides the explicit angular flux solution such that the physical picture for the SP{sub n} approximation is transparent and the SP{sub n} discontinuity factors can be calculated. (authors)

  9. Endothelin-1 stimulates cyclin D1 expression in rat cultured astrocytes via activation of Sp1.

    PubMed

    Michinaga, Shotaro; Ishida, Ayaka; Takeuchi, Risa; Koyama, Yutaka

    2013-07-01

    Endothelins (ETs), a family of vasoconstrictor peptides, are up-regulated in several pathological conditions in the brain, and induce astrocytic proliferation. We previously observed that ET-1 increased the expression of cyclin D1 protein. Thus, we confirmed the intracellular up-regulation of cyclin D1 by ET-1 in rat cultured astrocytes. Real-time PCR analysis indicated that ET-1 (100 nM) and Ala(1,3,11,15)-ET-1 (100 nM), a selective agonist of the ETB receptor, induced a time-dependent and transient increase in cyclin D1 mRNA. The effect of ET-1 was diminished by an ETB antagonist (1 ?M BQ788) or inhibitors of Sp1 (500 nM mithramycin), ERK (50 ?M PD98059), p38 (20 ?M SB203580) and JNK (1 ?M SP600125), but not inhibitors of NF-?B (10 ?M SN50 and 100 ?M pyrrolidine dithiocarbamate). The binding assay for Sp1 indicated that ET-1 increased the binding activity of Sp1 to consensus sequences, and two oligonucleotides of the cyclin D1 promoter including the Sp1-binding sites diminished the effect of ET-1. Western blot analysis showed that ET-1 induced time-dependent and transient phosphorylation of Sp1 on Thr453 and Thr739 via the ETB receptor. ET-1-induced phosphorylation of Sp1 was attenuated by PD98059 and SP600125. Additionally, ET-1 increased the incorporation of bromodeoxyuridine (BrdU) in cultured astrocytes and the number of BrdU-positive cells decreased in the presence of PD98059, SP600125 and mithramycin. These results suggest that ET-1 increases the expression of cyclin D1 via activation of Sp1 and induces astrocytic proliferation. PMID:23619396

  10. Generation of Enterobacter sp. YSU auxotrophs using transposon mutagenesis.

    PubMed

    Caguiat, Jonathan James

    2014-01-01

    Prototrophic bacteria grow on M-9 minimal salts medium supplemented with glucose (M-9 medium), which is used as a carbon and energy source. Auxotrophs can be generated using a transposome. The commercially available, Tn5-derived transposome used in this protocol consists of a linear segment of DNA containing an R6K? replication origin, a gene for kanamycin resistance and two mosaic sequence ends, which serve as transposase binding sites. The transposome, provided as a DNA/transposase protein complex, is introduced by electroporation into the prototrophic strain, Enterobacter sp. YSU, and randomly incorporates itself into this host's genome. Transformants are replica plated onto Luria-Bertani agar plates containing kanamycin, (LB-kan) and onto M-9 medium agar plates containing kanamycin (M-9-kan). The transformants that grow on LB-kan plates but not on M-9-kan plates are considered to be auxotrophs. Purified genomic DNA from an auxotroph is partially digested, ligated and transformed into a pir+ Escherichia coli (E. coli) strain. The R6K? replication origin allows the plasmid to replicate in pir+ E. coli strains, and the kanamycin resistance marker allows for plasmid selection. Each transformant possesses a new plasmid containing the transposon flanked by the interrupted chromosomal region. Sanger sequencing and the Basic Local Alignment Search Tool (BLAST) suggest a putative identity of the interrupted gene. There are three advantages to using this transposome mutagenesis strategy. First, it does not rely on the expression of a transposase gene by the host. Second, the transposome is introduced into the target host by electroporation, rather than by conjugation or by transduction and therefore is more efficient. Third, the R6K? replication origin makes it easy to identify the mutated gene which is partially recovered in a recombinant plasmid. This technique can be used to investigate the genes involved in other characteristics of Enterobacter sp. YSU or of a wider variety of bacterial strains. PMID:25408097

  11. Degradation of 3-phenylbutyric acid by Pseudomonas sp.

    PubMed Central

    Sariaslani, F S; Sudmeier, J L; Focht, D D

    1982-01-01

    Pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-PB) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. During early exponential growth, a catechol substance identified as 3-(2,3-dihydroxyphenyl)butyrate (2,3-DHPB) and its meta-cleavage product 2-hydroxy-7-methyl-6-oxononadioic-2,4-dienoic acid were produced. These products disappeared during late exponential growth, and considerable amounts of 2,3-DHPB reacted to form brownish polymeric substances. The catechol intermediate 2,3-DHPB could not be isolated, but cell-free extracts were able only to oxidize 3-(2,3-dihydroxyphenyl)propionate of all dihydroxy aromatic acids tested. Moreover, a reaction product caused by dehydration of 2,3-DHPB on silica gel was isolated and identified by spectral analysis as (--)-8-hydroxy-4-methyl-3,4-dihydrocoumarin. 3-Phenylpropionate and a hydroxycinnamate were found in supernatants of cultures grown on 3-PB; phenylacetate and benzoate were found in supernatants of cultures grown on 3-phenylpropionate; and phenylacetate was found in cultures grown on cinnamate. Cells grown on 3-PB rapidly oxidized 3-phenylpropionate, cinnamate, catechol, and 3-(2,3-dihydroxyphenyl)propionate, whereas 2-phenylpropionate, 2,3-dihydroxycinnamate, benzoate, phenylacetate, and salicylate were oxidized at much slower rates. Phenylsuccinate was not utilized for growth nor was it oxidized by washed cell suspensions grown on 3-PB. However, dual axenic cultures of Pseudomonas acidovorans and Klebsiella pneumoniae, which could not grow on phenylsuccinate alone, could grow syntrophically and produced the same metabolites found during catabolism of 3-PB by Pseudomonas sp. Washed cell suspensions of dual axenic cultures also immediately oxidized phenylsuccinate, 3-phenylpropionate, cinnamate, phenylacetate, and benzoate. PMID:7118830

  12. Sp100 Isoform-Specific Regulation of Human Adenovirus 5 Gene Expression

    PubMed Central

    Berscheminski, Julia; Wimmer, Peter; Brun, Juliane; Ip, Wing Hang; Groitl, Peter; Horlacher, Tim; Jaffray, Ellis; Hay, Ron T.; Schreiner, Sabrina

    2014-01-01

    ABSTRACT Promyelocytic leukemia nuclear bodies (PML-NBs) are nuclear structures that accumulate intrinsic host factors to restrict viral infections. To ensure viral replication, these must be limited by expression of viral early regulatory proteins that functionally inhibit PML-NB-associated antiviral effects. To benefit from the activating capabilities of Sp100A and simultaneously limit repression by Sp100B, -C, and -HMG, adenoviruses (Ads) employ several features to selectively and individually target these isoforms. Ads induce relocalization of Sp100B, -C, and -HMG from PML-NBs prior to association with viral replication centers. In contrast, Sp100A is kept at the PML tracks that surround the newly formed viral replication centers as designated sites of active transcription. We concluded that the host restriction factors Sp100B, -C, and -HMG are potentially inactivated by active displacement from these sites, whereas Sp100A is retained to amplify Ad gene expression. Ad-dependent loss of Sp100 SUMOylation is another crucial part of the virus repertoire to counteract intrinsic immunity by circumventing Sp100 association with HP1, therefore limiting chromatin condensation. We provide evidence that Ad selectively counteracts antiviral responses and, at the same time, benefits from PML-NB-associated components which support viral gene expression by actively recruiting them to PML track-like structures. Our findings provide insights into novel strategies for manipulating transcriptional regulation to either inactivate or amplify viral gene expression. IMPORTANCE We describe an adenoviral evasion strategy that involves isoform-specific and active manipulation of the PML-associated restriction factor Sp100. Recently, we reported that the adenoviral transactivator E1A targets PML-II to efficiently activate viral transcription. In contrast, the PML-associated proteins Daxx and ATRX are inhibited by early viral factors. We show that this concept is more intricate and significant than originally believed, since adenoviruses apparently take advantage of specific PML-NB-associated proteins and simultaneously inhibit antiviral measures to maintain the viral infectious program. Specifically, we observed Ad-induced relocalization of the Sp100 isoforms B, C, and HMG from PML-NBs juxtaposed with viral replication centers. In contrast, Sp100A is retained at Ad-induced PML tracks that surround the newly formed viral replication centers, acting as designated sites of active transcription. The host restriction factors Sp100B, -C, and -HMG are potentially inactivated by active displacement from these sites, whereas Sp100A is retained to amplify Ad gene expression. PMID:24623443

  13. Transcription Factors ETF, E2F, and SP-1 Are Involved in Cytokine-Independent Proliferation of Murine

    E-print Network

    Timmer, Jens

    Transcription Factors ETF, E2F, and SP-1 Are Involved in Cytokine-Independent Proliferation-regulated. The latter genes showed an overrepresen- tation of transcription factor binding sites (TFBS) for ETF (TEA domain family member 2), E2F1 (E2F transcription factor 1), and SP-1 (Sp1 transcription factor) (P

  14. DNA sequence comparisons of Ophiostoma spp., including Ophiostoma aurorae sp. nov., associated with pine bark beetles in

    E-print Network

    269 DNA sequence comparisons of Ophiostoma spp., including Ophiostoma aurorae sp. nov., associated for the first time and the new species, O. aurorae sp. nov., is described from pine-infesting bark beetles in South Africa. Taxonomic novelty: Ophiostoma aurorae X.D. Zhou & M.J. Wingf. sp. nov. Key words: Bark

  15. VEMBU STOREGRID SP4.2.1 SETUP (WINDOWS) Page 1 of 22 VEMBU STOREGRID CLIENT SETUP (WINDOWS PCs)

    E-print Network

    Zhou, Chongwu

    ) StoreGrid Password Download the StoreGrid client installation file for your Windows operating system on downloaded file (VembuStoreGrid_4_2_1_SP_CO.exe) and click on Run. #12;VEMBU STOREGRID SP4.2.1 SETUP (WINDOWSVEMBU STOREGRID SP4.2.1 SETUP (WINDOWS) Page 1 of 22 VEMBU STOREGRID CLIENT SETUP (WINDOWS PCs

  16. Pantoea gaviniae sp. nov. and Pantoea calida sp. nov., isolated from infant formula and an infant formula production environment.

    PubMed

    Popp, Alexandra; Cleenwerck, Ilse; Iversen, Carol; De Vos, Paul; Stephan, Roger

    2010-12-01

    Five Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped bacterial isolates were obtained from infant formula and an infant formula production environment and were investigated by use of a polyphasic taxonomic study. Biochemical tests and partial rpoB gene sequence analysis of the five isolates revealed that they formed two distinct groups in the family Enterobacteriaceae, closely related to several species of the genera Pantoea and Erwinia, which indicated a phylogenetic position within the genus Pantoea or the genus Erwinia. Multilocus sequence analysis of concatenated partial atpD, gyrB, infB and rpoB gene sequences of two of the isolates suggested that they represented two novel species of the genus Pantoea, phylogenetically related most closely to Pantoea septica. The five isolates had general characteristics consistent with those of the genus Pantoea, and DNA-DNA hybridizations between two representatives and the type strains of their phylogenetically closest relatives based on comparative 16S rRNA gene sequence analysis showed that the isolates represented two novel genospecies. These two genospecies could be differentiated from each other based on fermentation of galacturonate, sorbitol and potassium 5-ketogluconate. They could be differentiated from phylogenetically related Pantoea species based on their ability to ferment lactose and to utilize ?-gentiobiose and raffinose, their inability to ferment or utilize d-arabitol, and their inability to produce indole. On the basis of the results obtained, the five isolates are considered to represent two novel species of the genus Pantoea, for which the names Pantoea gaviniae sp. nov. (type strain A18/07(T) =LMG 25382(T) =DSM 22758(T)) and Pantoea calida sp. nov. (type strain 1400/07(T) =LMG 25383(T) =DSM 22759(T)) are proposed. PMID:20061487

  17. Deinococcus metallilatus sp. nov. and Deinococcus carri sp. nov., isolated from a car air-conditioning system.

    PubMed

    Kim, Dong-Uk; Lee, Hyosun; Lee, Ji-Hyeong; Ahn, Jae-Hyung; Lim, Sangyong; Jeong, Sunwook; Park, So Yoon; Seong, Chi Nam; Ka, Jong-Ok

    2015-09-01

    Two bacterial strains, designated MA1002T and MA1003T, were isolated from the air-conditioning system of a car. Cells of both strains were Gram-reaction-positive, non-motile, non-spore-forming coccoids, catalase- and oxidase-positive and UV-radiation resistant. The major fatty acids of strain MA1002T were iso-C17?:?0 and iso-C15?:?0 and those of strain MA1003T were iso-C16?:?0 and iso-C16?:?1 H. The polar lipid profile of MA1002T contained phosphatidylethanolamine, two unidentified phosphoglycolipids, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid and an unidentified lipid. MA1003T had three unidentified phosphoglycolipids, six unidentified phospholipids, two unidentified glycolipids and two unidentified polar lipids as the polar lipids. The G+C contents of the genomic DNA of MA1002T and MA1003T were 70.5 and 76.0?mol%, respectively. MK-8 was the predominant respiratory quinone for both strains. 16S rRNA gene sequence analysis showed that strain MA1002T was phylogenetically related to Deinococcus apachensis DSM 19763T, D. geothermalis DSM 11300T, D. aerius TR0125T and D. aetherius ST0316T (92.9, 92.6, 92.0 and 91.9?% sequence similarity, respectively), and MA1003T showed the highest sequence similarity to Deinococcus hopiensis KR-140T (92.9?%) and D. xinjiangensis X-82T (91.4?%). The results of genotypic and phenotypic characterizations showed that both strains could be distinguished from phylogenetically related species, and that the strains represented novel species within the genus Deinococcus, for which we propose the names Deinococcus metallilatus sp. nov. (type strain MA1002T?=?KACC 17964T?=?NBRC 110141T) and Deinococcus carri sp. nov. (type strain is MA1003T?=?KACC 17965T?=?NBRC 110142T). PMID:26297510

  18. New Species of Boletellus Section Boletellus (Boletaceae, Boletales) from Japan, B. aurocontextus sp. nov. and B. areolatus sp. nov.

    PubMed Central

    Sato, Hirotoshi; Hattori, Tsutomu

    2015-01-01

    We describe and illustrate two new species of Boletellus section Boletellus, B. aurocontextus sp. nov. and B. areolatus sp. nov., which are generally assumed to be B. emodensis. In this study, we reconstructed separate molecular phylogenetic trees of section Boletellus using the nucleotide sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA, the largest subunit (RPB1) and the second-largest subunit (RPB2) of nuclear RNA polymerase II gene and mitochondrial cytochrome oxidase subunit 3 (cox3) gene. We also examined the morphologies of B. emodensis sensu lato (s.l.) and other related species for comparison. The molecular phylogenetic tree inferred from the sequences of nuclear DNA (ITS, and combined dataset of RPB1 and RPB2) indicated that three genetically and phylogenetically well-separated lineages were present within B. emodensis s.l. These three lineages were also distinguished on the basis of the molecular phylogenetic tree constructed using the sequences of mitochondrial DNA (cox3), suggesting distinct cytonuclear disequilibria (i.e., evidence of reproductive isolation) among these lineages. Therefore, these three lineages can be treated as independent species: B. aurocontextus, B. areolatus, and B. emodensis. Boletellus aurocontextus and B. areolatus are also distinct from B. emodensis by the macro- and microscopic morphologies. Boletellus aurocontextus is characterized by a pileus with bright yellow to lemon yellow context, which can be observed through a gap in the scales, and basidiospores with relatively large length (mean spore length, 21.4 ?m; quotient of spore length and width, 2.51). In contrast, B. areolatus is characterized by a pileus with floccose to appressed thin scaly patches, a stipe with pallid or pale cream color at the upper half, and basidiospores with relatively small length (mean spore length, 16.5 ?m; quotient of spore length and width, 1.80). PMID:26083243

  19. Sphingomonas rosea sp. nov. and Sphingomonas swuensis sp. nov., rosy colored ?-glucosidase-producing bacteria isolated from soil.

    PubMed

    Srinivasan, Sathiyaraj; Lee, Jae-Jin; Kim, Myung Kyum

    2011-08-01

    Two strains PB196(T) and PB62(T) of Gram-negative, non-motile, and non-spore-forming bacteria, were isolated from soil in South Korea and characterized to determine their taxonomic positions. 16S rRNA gene sequence analysis showed that the two strains belonged to the genus Sphingomonas. The highest degree of sequence similarity of strain PB196(T) was found with PB62(T) (98.9%), Sphingomonas humi PB323(T) (98.9%), Sphingomonas kaistensis PB56(T) (98.2%), and Sphingomonas astaxanthinifaciens TDMA-17(T) (98.0%). The highest degree of sequence similarity of strain PB62(T) was found with Sphingomonas humi PB323(T) (98.8%), Sphingomonas astaxanthinifaciens TDMA-17(T) (98.2%), and Sphingomonas kaistensis PB56(T) (98.1%). Chemotaxonomic data revealed that they possessed ubiquinone-10 (Q-10) as common in the genus Sphingomonas, that the predominant fatty acids were summed feature 7 (C(18:1) ?7c/?9t/?12t), summed feature 4 (C(16:1) ?7c/C(15:0) iso 2OH), C(16:0), and C(17:1) ?6c, and that they contained sphingoglycolipid, phosphatidylglycerol (PG), and phosphatidyle-thanolamine (PE) in common but they showed difference for diphosphatidylglycerol (DPG). Based on these data, PB196(T) (=KCTC 12339(T) =JCM 16604(T)) and PB62(T) (=KCTC 12336(T) =JCM 16605(T) =KEMB 9004-005(T)) should be classified as type strains of two novel species, for which the names Sphingomonas rosea sp. nov. and Sphingomonas swuensis sp. nov. are proposed, respectively. PMID:21887644

  20. Calditerricola satsumensis gen. nov., sp. nov. and Calditerricola yamamurae sp. nov., extreme thermophiles isolated from a high-temperature compost.

    PubMed

    Moriya, Toshiyuki; Hikota, Tomohisa; Yumoto, Isao; Ito, Takashi; Terui, Yusuke; Yamagishi, Akihiko; Oshima, Tairo

    2011-03-01

    Two novel thermophilic micro-organisms, designated YMO81(T) and YMO722(T), were isolated from a high-temperature compost (internal temperature > 95 °C). The isolates were able to grow at 80 °C in a nutrient broth and in a synthetic medium. Cells were aerobic, Gram-negative rods (0.3 x 4.0 ?m). Spore formation was not observed. Strain YMO81(T) grew at 83 °C and pH 6.9-8.9 and grew optimally at 78 °C and pH 7.5 with 2 ?% NaCl. For growth in a synthetic minimal medium at 70 °C, the vitamins biotin, folic acid and thiamine and the amino acids glutamine and methionine were essential for growth of both strains; at 80 °C, strain YMO81(T) also required histidine, isoleucine, leucine, lysine, phenylalanine, serine, tryptophan and valine. Cellular fatty acids of the isolates comprised mainly iso-C??:? and anteiso-C??:?. The DNA G+C contents of strains YMO81(T) and YMO722(T) were 70 and 64 mol%, respectively. When the 16S rRNA gene sequences of the isolates were compared with those of other bacteria, highest similarity was observed with Planifilum yunnanense LA5(T) (90?% 16S rRNA gene sequence similarity). DNA-DNA relatedness between strain YMO722(T) and strain YMO81(T) was 55? %. N?-Aminopropylspermine was identified as a major polyamine, which suggested that the isolates were distinct from other related taxa. On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, we propose a new genus, Calditerricola gen. nov., and two novel species, the type species Calditerricola satsumensis sp. nov., with type strain YMO81(T) (=ATCC BAA-1462(T) =JCM 14719(T) =DSM 45223(T)), and Calditerricola yamamurae sp. nov., with type strain YMO722(T) (=ATCC BAA-1461(T) =JCM 14720(T) =DSM 45224(T)). PMID:20400666

  1. Natribaculum breve gen. nov., sp. nov. and Natribaculum longum sp. nov., halophilic archaea isolated from saline soil.

    PubMed

    Liu, Qin; Ren, Min; Zhang, Li-Li

    2015-02-01

    Two halophilic archaeal strains, TRM20010(T) and TRM20345(T), were isolated from saline soil of the Lop Nur region in Xinjiang, north-west China. Cells from the two strains were pleomorphic rods, stained Gram-negative and produced red-pigmented colonies. Strains TRM20010(T) and TRM20345(T) were able to grow at 30-62 °C (optimum 37 °C), 0.9-5.1 M NaCl (optimum 2.6 and 3.4 M, respectively) and pH 6.0-10.0 (optimum pH 7.0-7.5) and neither strain required Mg(2+) for growth. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), two glycolipids chromatographically identical to galactosyl mannosyl glucosyl diether (TGD-1) and disulfated mannosyl glucosyl diether (S2-DGD). Phylogenetic analysis based on 16S rRNA and rpoB' genes revealed that strains TRM20010(T) and TRM20345(T) clustered together and formed a distinct clade separated from the related genera Halovivax, Haloterrigena, Halostagnicola, Natronolimnobius and Natrinema. The DNA G+C contents of strains TRM20010(T) and TRM20345(T) were 63.9 and 63.8 mol%, respectively. The DNA-DNA hybridization value between strain TRM20010(T) and strain TRM20345(T) was 42.8 %. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strains TRM20010(T) and TRM20345(T) represent two novel species in a new genus within the family Halobacteriaceae, for which the names Natribaculum breve gen. nov., sp. nov. (type strain TRM20010(T)?= CCTCC AB2013112(T)?= NRRL B-59996(T)) and Natribaculum longum sp. nov. (type strain TRM20345(T)?= CCTCC AB2013113(T)?= NRRL B-59997(T)) are proposed. PMID:25406237

  2. Halomonas qijiaojingensis sp. nov. and Halomonas flava sp. nov., two moderately halophilic bacteria isolated from a salt lake.

    PubMed

    Chen, Chao; Shi, Rong; Liu, Bing-Bing; Zhang, Yun-Jiao; Sun, Hong-Zhuan; Li, Chang-Tian; Tang, Shu-Kun; Zhang, Li-Li; Li, Wen-Jun

    2011-10-01

    Two moderately halophilic, Gram-negative, rod-shaped bacteria, designated YIM 93003(T) and YIM 94343(T), were isolated from a salt lake in Xinjiang province, north-west China. The two strains YIM 93003(T) and YIM 94343(T) grew at 20-40°C, pH 6-9, 0.5-24% (w/v) NaCl and at 20-40°C, pH 6-9, 0.5-23% (w/v) NaCl, respectively. No growth occurred in absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains YIM 93003(T) and YIM 94343(T) were phylogenetically affiliated to the genus Halomonas and exhibited sequence similarity of 97.5% and 97.4% to the type strain Halomonas anticariensis DSM 16096(T), respectively. The strains possessed chemotaxonomic markers that were consistent with their classification in the genus Halomonas (Q-9 as predominant respiratory quinine; C18:1?7c, C16:0 and C16:1 ?7c/iso-C15:02-OH as the major fatty acids). The DNA-DNA hybridization values for strains YIM 93003(T) and YIM 94343(T), YIM 93003(T) and DSM 16096(T), YIM 94343(T) and DSM 16096(T) were 38.1 ± 3.0, 18.3 ± 4.7, and 20.8 ± 4.6%, respectively. The G+C contents of the strains YIM 93003(T) and YIM 94343(T) were 63.4 and 64.0 mol%, respectively. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA hybridization results, two novel species, Halomonas qijiaojingensis sp. nov., and Halomonas flava sp. nov., are proposed. The type strains are YIM 93003(T) (=CCTCC AB 208133(T) =KCTC 22228(T)) and YIM 94343(T) (=CCTCC AB 2010382(T) =KCTC 23356(T)), respectively. PMID:21656193

  3. Mycobacterium sediminis sp. nov. and Mycobacterium arabiense sp. nov., two rapidly growing members of the genus Mycobacterium.

    PubMed

    Zhang, Dao-Feng; Chen, Xiu; Zhang, Xiao-Mei; Zhi, Xiao-Yang; Yao, Ji-Cheng; Jiang, Yi; Xiong, Zhi; Li, Wen-jun

    2013-11-01

    Two novel isolates of rapidly growing, Gram-stain-positive, non-chromogenic species of the genus Mycobacterium, strain YIM M13028(T) from a sediment sample collected from the South China Sea (19° 30.261' N 111° 0.247' E) at a depth of 42 m and strain YIM 121001(T) from a coastal zone sand sample collected in Dubai, United Arab Emirates, were obtained in our laboratory. Their taxonomic positions were determined by a polyphasic approach. Good growth of the two strains was observed at 28 °C and pH 7.0 with 0-2?% NaCl on tryptic soy agar medium. Both strains formed round orange-red colonies, strain YIM M13028(T) had a rough surface, while YIM 121001(T) was smooth. Cellular fatty acids, whole-cell protein profiles and TLC analysis of their mycolic acids show significant differences from reference stains. Phenotypic characteristics and multilocus sequence analysis (MLSA) of 16S rRNA gene, hsp65, rpoB and 16S-23S internal transcribed spacer (ITS) sequences indicated that both strains YIM M13028(T) and YIM 121001(T) belong to the genus Mycobacterium. DNA-DNA hybridization values revealed a low relatedness (<70?%) of the two isolates with the type strains Mycobacterium neoaurum DSM 44074(T) and Mycobacterium hodleri DSM 44183(T). The low DNA-DNA hybridization values (40.4±3.5?%) between strains YIM M13028(T) and YIM 121001(T) and phenotypic distinctiveness indicated that the two strains were representatives of different novel species of the genus Mycobacterium. The names proposed for these novel species are Mycobacterium sediminis sp. nov. and Mycobacterium arabiense sp. nov., and the type strains are YIM M13028(T) (?=?DSM 45643(T)?=?KCTC 19999(T)) and YIM 121001(T) (?=?DSM 45768(T)?=?JCM 18538(T)), respectively. PMID:23728378

  4. Neptunomonas phycophila sp. nov. isolated from a culture of Symbiodinium sp., a dinoflagellate symbiont of the sea anemone Aiptasia tagetes.

    PubMed

    Frommlet, Jörg; Guimarães, Bárbara; Sousa, Lígia; Serôdio, João; Alves, Artur

    2015-03-01

    A Gram-stain-negative, facultatively anaerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain SYM1(T), was isolated from a culture of Symbiodinium sp., an algal symbiont of the sea anemone Aiptasia tagetes collected in Puerto Rico. Growth was observed at 4-40 °C (optimum 30 °C), at pH 5.0-11.0 (optimum pH 8.0) and with 0.5-8?% (optimum 2?%) (w/v) NaCl. Phylogenetic analyses of 16S rRNA gene sequences showed that strain SYM1(T) was a member of the genus Neptunomonas with the type strain of Neptunomonas naphthovorans as the closest phylogenetic relative with a pairwise sequence similarity of 98.15?%. However, DNA-DNA relatedness between strain SYM1(T) and N. naphthovorans CIP 106451(T) was 24?%. Moreover, strain SYM1(T) could be distinguished from its closest relative by several phenotypic characteristics such as NaCl, pH and temperature tolerance, nitrate reduction and utilization of carbon substrates. The major cellular fatty acids were C16?:?0, C18?:?1?7c and summed feature 3 (comprising C16?:?1?7c and/or iso-C15?:?0 2-OH). The genomic DNA G+C content of strain SYM1(T) was 45 mol%. Ubiquinone-8 (Q-8) was the only respiratory quinone detected. Based on a polyphasic taxonomic characterization, strain SYM1(T) represents a novel species of the genus Neptunomonas, for which the name Neptunomonas phycophila sp. nov. is proposed. The type strain is SYM1(T) (?=?LMG 28329(T)?=?CECT 8716(T)). PMID:25563909

  5. The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

    PubMed Central

    2013-01-01

    Background Six independent Gram-negative, facultatively anaerobic, non-spore-forming, nitrogen-fixing rod-shaped isolates were obtained from the root endosphere of rice grown at the International Rice Research Institute (IRRI) and investigated in a polyphasic taxonomic study. Results The strains produced fatty acid patterns typical for members of the family Enterobacteriaceae. Comparative sequence analyses of the 16S rRNA as well as rpoB genes allocated the strains to two well-defined groups within the genus Enterobacter, family Enterobacteriaceae. The analyses indicated Enterobacter radicincitans, Enterobacter arachidis and Enterobacter oryzae to be the closest related species. An RpoB (translated) protein comparison supported the placement in the genus Enterobacter and the relatedness of our isolates to the aforementioned species. Genomic DNA:DNA hybridization analyses and biochemical analyses provided further evidence that the novel strains belong to two new species within the genus Enterobacter. The two species can be differentiated from each other and from existing enteric species by acid production from L-rhamnose and D-melibiose, decarboxylation of ornithine and utilization of D-alanine, D-raffinose L-proline and L-aspartic acid, among other characteristics. Members of both species revealed capacities to colonise rice roots, including plant-growth-promoting capabilities such as an active supply of fixed nitrogen to the plant and solubilisation of inorganic phosphorus, next to traits allowing adaptation to the plant. Conclusions Two novel proposed enterobacterial species, denominated Enterobacter oryziphilus sp. nov. (type strain REICA_142T=LMG 26429T=NCCB 100393T) and Enterobacter oryzendophyticus sp. nov. (type strain REICA_082T=LMG 26432T =NCCB 100390T) were isolated from rice roots. Both species are capable of promoting rice growth by supplying nitrogen and phosphorus. PMID:23865888

  6. Negative Regulation of DsbA-L Gene Expression by the Transcription Factor Sp1

    PubMed Central

    Fang, Qichen; Yang, Wenjing; Li, Huating; Hu, Wenxiu; Chen, Lihui; Jiang, Shan; Dong, Kun; Song, Qianqian; Wang, Chen; Chen, Shuo; Liu, Feng

    2014-01-01

    Disulfide-bond A oxidoreductase-like protein (DsbA-L) possesses beneficial effects such as promoting adiponectin multimerization and stability, increasing insulin sensitivity, and enhancing energy metabolism. The expression level of DsbA-L is negatively correlated with obesity in mice and humans, but the underlying mechanisms remain unknown. To address this question, we generated reporter gene constructs containing the promoter sequence of the mouse DsbA-L gene. Deletion analysis showed that the proximal promoter of mouse DsbA-L is located between ?186 and ?34 bp relative to the transcription start site. In silico analysis identified a putative Sp1 transcription factor binding site in the first intron of the DsbA-L gene. Electrophoretic mobility shift assay and chromatin immunoprecipitation analysis indicated that Sp1 bound to this intron region in vitro and in intact cells. Overexpression of Sp1 or suppressing Sp1 expression by siRNA reduced or increased DsbA-L promoter activity, respectively. The binding activity of Sp1 was gradually decreased during 3T3-L1 cell differentiation and was significantly increased in adipose tissues of obese mice. Our results identify Sp1 as an inhibitor of DsbA-L gene transcription, and the Sp1-mediated inhibition of DsbA-L gene expression may provide a mechanism underlying obesity-induced adiponectin downregulation and insulin resistance. PMID:25024375

  7. Glutamine protects against apoptosis via downregulation of Sp3 in intestinal epithelial cells.

    PubMed

    Ban, Kechen; Kozar, Rosemary A

    2010-12-01

    Glutamine plays a key role in intestinal growth and maintenance of gut function, and as we have shown protects the postischemic gut (Kozar RA, Scultz SG, Bick RJ, Poindexter BJ, Desoigne R, Weisbrodt NW, Haber MM, Moore FA. Shock 21: 433-437, 2004). However, the precise mechanisms of the gut protective effects of glutamine have not been well elucidated. In the present study, RNA microarray was performed to obtain differentially expressed genes in intestinal epithelial IEC-6 cells following either 2 mM or 10 mM glutamine. The result demonstrated that specificity protein 3 (Sp3) mRNA expression was downregulated 3.1-fold. PCR and Western blot confirmed that Sp3 expression was decreased by glutamine in a time- and dose-dependent fashion. To investigate the role of Sp3, Sp3 gene siRNA silencing was performed and apoptosis was assessed. Silencing of Sp3 demonstrated a significant increase in Bcl-2 and decrease in Bax protein expression, as well as a decrease in caspase-3, -8, and -9 protein expression and activity. The protein expression of apoptosis-related proteins after hypoxia/reoxygenation was similar to that of normoxia and correlated with a decrease in DNA fragmentation. Importantly, the addition of glutamine to Sp3-silenced cells did not further lessen apoptosis, suggesting that Sp3 plays a major role in the inhibitory effect of glutamine on apoptosis. This novel finding may explain in part the gut-protective effects of glutamine. PMID:20884886

  8. The Effect of Desulfovibrio sp. Biofilms on Corrosion Behavior of Copper in Sulfide-Containing Solutions

    NASA Astrophysics Data System (ADS)

    Güngör, Nihal Do?ruöz; Çotuk, Ay??n; D??p?nar, Derya

    2015-03-01

    This study aims to detect the effect of Desulfovibrio sp. on copper in terms of biofilm formation and corrosion in 722 h. In that way, appropriate strategies to inhibit microbiological corrosion in copper systems with Desulfovibrio sp. can be evaluated. For this purpose, experiments were performed in 1 L glass model system containing 28 copper coupons and pure culture of the sulfate-reducing bacteria (SRB) strain Desulfovibrio sp. in Postgate's medium C. Also, a control system with copper coupons but without Desulfovibrio sp. containing sterile Postgate's medium was studied concurrently with the test system. The test coupons were collected from systems at certain time intervals, namely 24, 168, 360, and 720 h. The samples were then subjected to several characterization analyses such as measurement of Desulfovibrio sp. numbers, corrosion resistance, EPS extraction, carbohydrate analysis, SEM, and EDS. During the experiments, the maximum Desulfovibrio sp. count in biofilm samples was found at 360 h. Carbohydrate and copper concentrations in biofilm were increased over time. EDS analysis revealed Cu, S, C, O, and Cl peaks on the surface of the samples. For the control coupons, only Cu peaks were observed. The results obtained from this study showed that copper was corroded by Desulfovibrio sp. in the model system under laboratory conditions.

  9. Hesperidin Induces Apoptosis by Inhibiting Sp1 and Its Regulatory Protein in MSTO-211H Cells

    PubMed Central

    Lee, Kyung-Ae; Lee, Sang-Han; Lee, Yong-Jin; Baeg, Seung Mi; Shim, Jung-Hyun

    2012-01-01

    Hesperidin, a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-tumor activity and apoptotic effects in several cancers, but there is no report about the apoptotic effect of hesperidin in human malignant pleural mesothelioma through the specificity protein 1 (Sp1) protein. We investigated whether hesperidin inhibited cell growth and regulated Sp1 target proteins by suppressing the levels of Sp1 protein in MSTO-211H cells. The IC50 value of hesperidin was determined to be 152.3 ?M in MSTO-211H cells for 48 h. Our results suggested that hesperidin (0-160 ?M) decreased cell viability, and induced apoptotic cell death. Hesperidin increased Sub-G1 population in MSTO-211H cells. Hesperidin significantly suppressed mRNA/protein level of Sp1 and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in mesothelioma cells. Also, hesperidin induced apoptotic signaling including: cleavages of Bid, caspase-3, and PARP, upregulation of Bax, and down-regulation of Bcl-xl in mesothelioma cells. These results show that hesperidin suppressed mesothelioma cell growth through inhibition of Sp1. In this study, we demonstrated that Sp1 acts as a novel molecular target of hesperidin in human malignant pleural mesothelioma. PMID:24130923

  10. SP-D, KL-6, and HTI-56 levels in children with mycoplasma pneumoniae pneumonia

    PubMed Central

    Shu, Lin-Hua; Lu, Quan; Han, Li-Ying; Dong, Guang-Hui

    2015-01-01

    The study was aimed to evaluate the potential biomarkers from pulmonary surfactant protein D (SP-D), Krebs von den Lungen-6 (KL-6), and 56-kD a human type I protein (HTI-56) in serum and bronchoalveolar lavage fluid samples of children with Mycoplasma pneumoniae pneumonia. This retrospective study, self-controlled study enrolled 34 Chinese children with M. pneumoniae pneumonia. The levels of SP-D, KL-6, and HTI-56 in bronchoalveolar lavage fluid samples were assessed and compared between patients with unilateral lung infection and contralateral lungs without any abnormal findings. Significant differences in the levels of SP-D, KL-6, and HTI-56 were observed in infected bronchoalveolar lavage fluid samples compared with uninfected samples (all P<0.05); however, there was no correlation between the serum level of SP-D, KL-6, and HTI-56 and their levels in infected and uninfected bronchoalveolar lavage fluid samples (P>0.05). Conclusion: The high levels of SP-D, KL-6, and HTI-56 in infected bronchoalveolar lavage fluid samples may reflect the injury of alveolar epithelium caused by M. pneumoniae. Instead of SP-D in uninfected bronchoalveolar lavage fluid samples obtained by invasive bronchoscopy, serum SP-D may serve as a convenient medium to distinguish lung infection caused by M. pneumoniae.

  11. KLF/SP Transcription Factor Family Evolution: Expansion, Diversification, and Innovation in Eukaryotes.

    PubMed

    Presnell, Jason S; Schnitzler, Christine E; Browne, William E

    2015-08-01

    The Krüppel-like factor and specificity protein (KLF/SP) genes play key roles in critical biological processes including stem cell maintenance, cell proliferation, embryonic development, tissue differentiation, and metabolism and their dysregulation has been implicated in a number of human diseases and cancers. Although many KLF/SP genes have been characterized in a handful of bilaterian lineages, little is known about the KLF/SP gene family in nonbilaterians and virtually nothing is known outside the metazoans. Here, we analyze and discuss the origins and evolutionary history of the KLF/SP transcription factor family and associated transactivation/repression domains. We have identified and characterized the complete KLF/SP gene complement from the genomes of 48 species spanning the Eukarya. We have also examined the phylogenetic distribution of transactivation/repression domains associated with this gene family. We report that the origin of the KLF/SP gene family predates the divergence of the Metazoa. Furthermore, the expansion of the KLF/SP gene family is paralleled by diversification of transactivation domains via both acquisitions of pre-existing ancient domains as well as by the appearance of novel domains exclusive to this gene family and is strongly associated with the expansion of cell type complexity. PMID:26232396

  12. Cryptosporidium huwi n. sp. (Apicomplexa: Eimeriidae) from the guppy (Poecilia reticulata).

    PubMed

    Ryan, Una; Paparini, Andrea; Tong, Kaising; Yang, Rongchang; Gibson-Kueh, Susan; O'Hara, Amanda; Lymbery, Alan; Xiao, Lihua

    2015-03-01

    The morphological, biological, and molecular characteristics of Cryptosporidium piscine genotype 1 from the guppy (Poecilia reticulata) are described, and the species name Cryptosporidium huwi n. sp. is proposed to reflect its genetic and biological differences from gastric and intestinal Cryptosporidium species. Oocysts of C.huwi n. sp. over-lap in size with Cryptosporidium molnari, measuring approximately 4.4-4.9?µm (mean 4.6) by 4.0-4.8?µm (mean 4.4?µm) with a length to width ratio of 1.04 (0.92-1.35) (n?=?50). Similar to C.molnari, C.huwi n. sp. was identified in the stomach only and clusters of oogonial and sporogonial stages were identified deep within the epithelium. However, phylogenetic analysis of 18S rRNA sequences indicated that C. huwi n. sp. exhibited 8.5-9.2% and 3.5% genetic distance from C.molnari isolates and piscine genotype 7 respectively. At the actin locus, the genetic distance between C.huwi n. sp. and C.molnari was 16.6%. The genetic distance between C.huwi n. sp. and other Cryptosporidium species at the 18S locus was 13.2%-17% and at the actin locus was 18.9%-26.3%. Therefore C. huwi n. sp. is genetically distinct from previously described Cryptosporidium species. PMID:25637783

  13. KLF/SP Transcription Factor Family Evolution: Expansion, Diversification, and Innovation in Eukaryotes

    PubMed Central

    Presnell, Jason S.; Schnitzler, Christine E.; Browne, William E

    2015-01-01

    The Krüppel-like factor and specificity protein (KLF/SP) genes play key roles in critical biological processes including stem cell maintenance, cell proliferation, embryonic development, tissue differentiation, and metabolism and their dysregulation has been implicated in a number of human diseases and cancers. Although many KLF/SP genes have been characterized in a handful of bilaterian lineages, little is known about the KLF/SP gene family in nonbilaterians and virtually nothing is known outside the metazoans. Here, we analyze and discuss the origins and evolutionary history of the KLF/SP transcription factor family and associated transactivation/repression domains. We have identified and characterized the complete KLF/SP gene complement from the genomes of 48 species spanning the Eukarya. We have also examined the phylogenetic distribution of transactivation/repression domains associated with this gene family. We report that the origin of the KLF/SP gene family predates the divergence of the Metazoa. Furthermore, the expansion of the KLF/SP gene family is paralleled by diversification of transactivation domains via both acquisitions of pre-existing ancient domains as well as by the appearance of novel domains exclusive to this gene family and is strongly associated with the expansion of cell type complexity. PMID:26232396

  14. Genetic variation in SP-A2 leads to differential binding to Mycoplasma pneumoniae membranes and regulation of host responses.

    PubMed

    Ledford, Julie G; Voelker, Dennis R; Addison, Kenneth J; Wang, Ying; Nikam, Vinayak S; Degan, Simone; Kandasamy, Pitachaimani; Tanyaratsrisakul, Sasipa; Fischer, Bernard M; Kraft, Monica; Hollingsworth, John W

    2015-06-15

    Mycoplasma pneumoniae is an extracellular pathogen that colonizes mucosal surfaces of the respiratory tract and is associated with asthma exacerbations. Previous reports demonstrate that surfactant protein-A (SP-A) binds live M. pneumoniae and mycoplasma membrane fractions (MMF) with high affinity. Humans express a repertoire of single-amino acid genetic variants of SP-A that may be associated with lung disease, and our findings demonstrate that allelic differences in SP-A2 (Gln223Lys) affect the binding to MMF. We show that SP-A(-/-) mice are more susceptible to MMF exposure and have significant increases in mucin production and neutrophil recruitment. Novel humanized SP-A2-transgenic mice harboring the hSP-A2 223K allele exhibit reduced neutrophil influx and mucin production in the lungs when challenged with MMF compared with SP-A(-/-) mice. Conversely, mice expressing hSP-A2 223Q have increased neutrophil influx and mucin production that are similar to SP-A(-/-) mice. Using tracheal epithelial cell cultures, we show that enhanced mucin production to MMF occurs in the absence of SP-A and is not dependent upon neutrophil recruitment. Increased phosphorylation of the epidermal growth factor receptor (EGFR) was evident in the lungs of MMF-challenged mice when SP-A was absent. Pharmacologic inhibition of EGFR prior to MMF challenge dramatically reduced mucin production in SP-A(-/-) mice. These findings suggest a protective role for SP-A in limiting MMF-stimulated mucin production that occurs through interference with EGFR-mediated signaling. SP-A interaction with the EGFR signaling pathway appears to occur in an allele-specific manner that may have important implications for SP-A polymorphisms in human diseases. PMID:25957169

  15. Poster — Thur Eve — 47: Monte Carlo Simulation of Scp, Sc and Sp

    SciTech Connect

    Zhan, Lixin; Jiang, Runqing; Osei, Ernest K.

    2014-08-15

    The in-water output ratio (Scp), in-air output ratio (Sc), and phantom scattering factor (Sp) are important parameters for radiotherapy dose calculation. Experimentally, Scp is obtained by measuring the dose rate ratio in water phantom, and Sc the water Kerma rate ratio in air. There is no method that allows direct measurement of Sp. Monte Carlo (MC) method has been used to simulate Scp and Sc in literatures, similar to experimental setup, but no MC direct simulation of Sp available yet to the best of our knowledge. We propose in this report a method of performing direct MC simulation of Sp. Starting from the definition, we derived that Sp of a clinical photon beam can be approximated by the ratio of the dose rates contributed from the primary beam for a given field size to the reference field size. Since only the primary beam is used, any Linac head scattering should be excluded from the simulation, which can be realized by using the incident electron as a scoring parameter for MU. We performed MC simulations for Scp, Sc and Sp. Scp matches well with golden beam data. Sp obtained by the proposed method agrees well with what is obtained using the traditional method, Sp=Scp/Sc. Since the smaller the field size, the more the primary beam dominates, our Sp simulation method is accurate for small field. By analyzing the calculated data, we found that this method can be used with no problem for large fields. The difference it introduced is clinically insignificant.

  16. P63 (CKAP4) as an SP-A Receptor: Implications for Surfactant Turnover

    PubMed Central

    Bates, Sandra R.

    2009-01-01

    Surfactant protein-A (SP-A) plays an important role in the clearance of surfactant from the lung alveolar space and in the regulation of surfactant secretion and uptake by type II pneumocytes in culture. Two pathways are important for the endocytosis of surfactant by type II cells and the intact lung, a receptor-mediated clathrin-dependent pathway and a non-clathrin, actin-mediated pathway. The critical role of the clathrin/receptor-mediated pathway in normal mice is supported by the finding that SP-A gene-targeted mice use the actin-dependent pathway to maintain normal clearance of surfactant. Addition of SP-A to the surfactant of the SP-A null mice “rescued” the phenotype, further emphasizing the essential role of the SP-A/receptor-mediated process in surfactant turnover. This review presents an overview of the structure of SP-A and its function in surfactant turnover. The evidence that the interaction of SP-A with type II cells is a receptor-mediated process is presented. A newly identified receptor for SP-A, P63/CKAP4, is described in detail, with elucidation of the specific structural features of this 63 kDa, nonglycosylated, highly coiled, transmembrane protein. The compelling evidence that P63 functions as a receptor for SP-A on type II cells is summarized. Regulation of P63 receptor density on the surface of pneumocytes may be a novel approach for the regulation of surfactant homeostasis by the lung. PMID:20054143

  17. Serine proteases SP1 and SP13 mediate the melanization response of Asian corn borer, Ostrinia furnacalis, against entomopathogenic fungus Beauveria bassiana.

    PubMed

    Chu, Yuan; Liu, Yang; Shen, Dongxu; Hong, Fang; Wang, Guirong; An, Chunju

    2015-06-01

    Exposure to entomopathogenic fungi is one approach for insect pest control. Little is known about the immune interactions between fungus and its insect host. Melanization is a prominent immune response in insects in defending against pathogens such as bacteria and fungi. Clip domain serine proteases in insect plasma have been implicated in the activation of prophenoloxidase, a key enzyme in the melanization. The relationship between host melanization and the infection by a fungus needs to be established. We report here that the injection of entomopathogenic fungus Beauveria bassiana induced both melanin synthesis and phenoloxidase activity in its host insect, the Asian corn borer, Ostrinia furnacalis (Guenée). qRT-PCR analysis showed several distinct patterns of expression of 13 clip-domain serine proteases in response to the challenge of fungi, with seven increased, two decreased, and four unchanged. Of special interest among these clip-domain serine protease genes are SP1 and SP13, the orthologs of Manduca sexta HP6 and PAP1 which are involved in the prophenoloxidase activation pathway. Recombinant O. furnacalis SP1 was found to activate proSP13 and induce the phenoloxidase activity in corn borer plasma. Additionally, SP13 was determined to directly cleave prophenoloxidase and therefore act as the prophenoloxidase activating protease. Our work thus reveals a biochemical mechanism in the melanization in corn borer associated with the challenge by B. bassiana injection. These insights could provide valuable information for better understanding the immune responses of Asian corn borer against B. bassiana. PMID:25900291

  18. IAA-producing Penicillium sp. NICS01 triggers plant growth and suppresses Fusarium sp.-induced oxidative stress in sesame (Sesamum indicum L.).

    PubMed

    Radhakrishnan, Ramalingam; Shim, Kang-Bo; Lee, Byeong-Won; Hwang, Chung-Dong; Pae, Suk-Bok; Park, Chang-Hwan; Kim, Sung-Up; Lee, Choon-Ki; Baek, In-Youl

    2013-06-28

    Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growthpromoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation. PMID:23676921

  19. Systematics and species-specific response to pH of Oxytricha acidotolerans sp. nov. and Urosomoida sp. (Ciliophora, Hypotricha) from acid mining lakes

    PubMed Central

    Weisse, Thomas; Moser, Michael; Scheffel, Ulrike; Stadler, Peter; Berendonk, Thomas; Weithoff, Guntram; Berger, Helmut

    2013-01-01

    We investigated the morphology, phylogeny of the 18S rDNA, and pH response of Oxytricha acidotolerans sp. nov. and Urosomoida sp. (Ciliophora, Hypotricha) isolated from two chemically similar acid mining lakes (pH ? 2.6) located at Langau, Austria, and in Lusatia, Germany. Oxytricha acidotolerans sp. nov. from Langau has 18 frontal-ventral-transverse cirri but a very indistinct kinety 3 fragmentation so that the assignment to Oxytricha is uncertain. The somewhat smaller species from Lusatia has a highly variable cirral pattern and the dorsal kineties arranged in the Urosomoida pattern and is, therefore, preliminary designated as Urosomoida sp. The pH response was measured as ciliate growth rates in laboratory experiments at pH ranging from 2.5 to 7.0. Our hypothesis was that the shape of the pH reaction norm would not differ between these closely related (3% difference in their SSU rDNA) species. Results revealed a broad pH niche for O. acidotolerans, with growth rates peaking at moderately acidic conditions (pH 5.2). Cyst formation was positively and linearly related to pH. Urosomoida sp. was more sensitive to pH and did not survive at circumneutral pH. Accordingly, we reject our hypothesis that similar habitats would harbour ciliate species with virtually identical pH reaction norm. PMID:23021638

  20. Involvement of the GC-rich sequence and specific proteins (Sp1/Sp3) in the basal transcription activity of neurogranin gene

    SciTech Connect

    Gui Jingang; Song Yan; Han, N.-L.R.; Zhou Shufeng; Sheu, F.-S. . E-mail: dbssfs@nus.edu.sg

    2006-06-23

    Neurogranin (Ng), a neuronal protein implicated in learning and memory, contains a TATA-less promoter. Analysis of 5'-deletion mutations and site-directed mutations of the mouse Ng promoter revealed that a 258 bp 5'-flanking sequence (+3 to +260) conferred the basal transcription activity, and that the GC-rich sequence (+22 to +33) served as an important determinant of the promoter activity. Transient transfection of the Sp1 expression plasmid transactivated the reporter activity in neuroblastoma N2A cells while knocking down of endogenous Sp1 expression resulted in a 2.5-fold reduction of the reporter activity in HEK 293 cells. Exogenous expression of Sp3 in HEK 293 cells, however, repressed the reporter activity by 50%. Nevertheless, by gel shift assays, Sp1 and Sp3 were not found to be responsible for the protein-DNA complexes formed by the GC-rich sequence. Moreover, a nuclear factor from the mouse brain tissues was discovered to bind to multiple AT-rich regions in Ng promoter.

  1. Hybrid nanocarbon as a catalyst for direct dehydrogenation of propane: formation of an active and selective core-shell sp2/sp3 nanocomposite structure.

    PubMed

    Wang, Rui; Sun, Xiaoyan; Zhang, Bingsen; Sun, Xiaoying; Su, Dangsheng

    2014-05-19

    Hybrid nanocarbon, comprised of a diamond core and a graphitic shell with a variable sp(2)-/sp(3)-carbon ratio, is controllably obtained through sequential annealing treatment (550-1300?°C) of nanodiamond. The formation of sp(2) carbon increases with annealing temperature and the nanodiamond surface is reconstructed from amorphous into a well-ordered, onion-like carbon structure via an intermediate composite structure--a diamond core covered by a defective, curved graphene outer shell. Direct dehydrogenation of propane shows that the sp(2)-/sp(3)-nanocomposite exhibits superior catalytic performance to that of individual nanodiamond and graphitic nanocarbon. The optimum catalytic activity of the diamond/graphene composite depends on the maximum structural defectiveness and high chemical reactivity of the ketone groups. Ketone-type functional groups anchored on the defects/vacancies are active for propene formation; nevertheless, once the oxygen functional groups are desorbed, the defects/vacancies alone might be active sites responsible for the C-H bond activation of propane. PMID:24740731

  2. Coordinating activation strategy for C(sp3)–H/C(sp3)–H cross-coupling to access ?-aromatic ?-amino acids

    PubMed Central

    Li, Kaizhi; Wu, Qian; Lan, Jingbo; You, Jingsong

    2015-01-01

    The past decade has witnessed significant advances in C–H bond functionalizations with the discovery of new mechanisms. Non-precious transition-metal-catalysed radical oxidative coupling for C(sp3)–H bond transformations is an appealing strategy for C–C bond formations. The radical oxidative C(sp3)–H/C(sp3)–H cross-coupling reactions of ?-C(sp3)–H bonds of amines with free radicals represent a conceptual and practical challenge. We herein develop the coordinating activation strategy to illustrate the nickel-catalysed radical oxidative cross-coupling between C(sp3)–H bonds and (hetero)arylmethyl free radicals. The protocol can tolerate a rich variety of ?-amino acids and (hetero)arylmethanes as well as arylmethylenes and arylmethines, affording a large library of ?-tertiary and ?-quaternary ?-aromatic ?-amino acids. This process also features low-cost metal catalyst, readily handled and easily removable coordinating group, synthetic simplicity and gram-scale production, which would enable the potential for economical production at commercial scale in the future. PMID:26415985

  3. O-GlcNAc inhibits interaction between Sp1 and Elf-1 transcription factors

    SciTech Connect

    Lim, Kihong; Chang, Hyo-Ihl

    2009-03-13

    The novel protein modification, O-linked N-acetylglucosamine (O-GlcNAc), plays an important role in various aspects of cell regulation. Although most of nuclear transcription regulatory factors are modified by O-GlcNAc, O-GlcNAc effects on transcription remain largely undefined yet. In this study, we show that O-GlcNAc inhibits a physical interaction between Sp1 and Elf-1 transcription factors, and negatively regulates transcription of placenta and embryonic expression oncofetal protein gene (Pem). These findings suggest that O-GlcNAc inhibits Sp1-mediated gene transcription possibly by interrupting Sp1 interaction with its cooperative factor.

  4. Rhodium(III)-Catalyzed Amidation of Unactivated C(sp(3) )?H Bonds.

    PubMed

    Wang, He; Tang, Guodong; Li, Xingwei

    2015-10-26

    Nitrogenation by direct functionalization of C?H bonds represents an important strategy for constructing C?N bonds. Rhodium(III)-catalyzed direct amidation of unactivated C(sp(3) )?H bonds is rare, especially under mild reaction conditions. Herein, a broad scope of C(sp(3) )?H bonds are amidated under rhodium catalysis in high efficiency using 3-substituted 1,4,2-dioxazol-5-ones as the amide source. The protocol broadens the scope of rhodium(III)-catalyzed C(sp(3) )?H activation chemistry, and is applicable to the late-stage functionalization of natural products. PMID:26480337

  5. Ni(II)/BINOL-catalyzed alkenylation of unactivated C(sp(3))-H bonds.

    PubMed

    Liu, Yue-Jin; Zhang, Zhuo-Zhuo; Yan, Sheng-Yi; Liu, Yan-Hua; Shi, Bing-Feng

    2015-05-01

    The first nickel-catalyzed alkenylation of unactivated C(sp(3))-H bonds with vinyl iodides is described. The catalytic system comprises an inexpensive and air-stable Ni(acac)2 as the catalyst and BINOL as the ligand, which is highly efficient for the alkenylation of ?-methyl C(sp(3))-H bonds of a broad range of aliphatic carboxamides. The resulting olefins can serve as versatile handles for further preparation. Additionally, we also demonstrated the synthesis of functionalized carboxamides bearing ?-quaternary carbon centers from simple pivalamide via nickel-catalyzed sequential C(sp(3))-H bond functionalization. PMID:25857332

  6. Bonding and hardness in nonhydrogenated carbon films with moderate sp{sup 3} content

    SciTech Connect

    Gago, R.; Jimenez, I.; Albella, J. M.; Climent-Font, A.; Caceres, D.; Vergara, I.; Banks, J. C.; Doyle, B. L.; Terminello, L. J.

    2000-06-01

    Amorphous carbon films with an sp{sup 3} content up to 25% and a negligible amount of hydrogen have been grown by evaporation of graphite with concurrent Ar{sup +} ion bombardment. The sp{sup 3} content is maximized for Ar{sup +} energies between 200 and 300 eV following a subplantation mechanism. Higher ion energies deteriorate the film due to sputtering and heating processes. The hardness of the films increases in the optimal assisting range from 8 to 18 GPa, and is explained by crosslinking of graphitic planes through sp{sup 3} connecting sites. (c) 2000 American Institute of Physics.

  7. Mechanism of Nickel(II)-Catalyzed Oxidative C(sp(2) )-H/C(sp(3) )-H Coupling of Benzamides and Toluene Derivatives.

    PubMed

    Xu, Zheng-Yang; Jiang, Yuan-Ye; Yu, Hai-Zhu; Fu, Yao

    2015-11-01

    The Ni-catalyzed C(sp(2) )-H/C(sp(3) )-H coupling of benzamides with toluene derivatives was recently successfully achieved with mild oxidant iC3 F7 I. Herein, we employ density functional theory (DFT) methods to resolve the mechanistic controversies. Two previously proposed mechanisms are excluded, and our proposed mechanism involving iodine-atom transfer (IAT) between iC3 F7 I and the Ni(II) intermediate was found to be more feasible. With this mechanism, the presence of a carbon radical is consistent with the experimental observation that (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) completely quenches the reaction. Meanwhile, the hydrogen-atom abstraction of toluene is irreversible and the activation of the C(sp(2) )-H bond of benzamides is reversible. Both of these conclusions are in good agreement with Chatani's deuterium-labeling experiments. PMID:26307522

  8. Antennal Sensilla in the Parasitoid Sclerodermus sp. (Hymenoptera: Bethylidae)

    PubMed Central

    Zhou, Chang-Xiang; Sun, Xiao; Mi, Feng; Chen, Jingyuan; Wang, Man-Qun

    2015-01-01

    Parasitoid wasps of the genus Sclerodermus (Hymenoptera: Bethylidae) are an important natural enemy of the Japanese pine sawyer beetle Monochamus alternatus Hope (Coleoptera: Cerambycidae). In this study, we used scanning electron microscopy to examine the external morphology of the antennal sensilla of Sclerodermus sp. Antennae of females and males comprised the scape, pedicel, and 11 flagellomere segments. Based on the morphology of the sensilla in each sex, seven types of sensillum were identified: sensilla trichodea (Tr.1, Tr.2 and Tr.3), sensilla basiconica (Ba.1, Ba.2, and Ba.3), sensilla styloconica (St.1 and St.2), sensilla placodea, sensilla coeloconica, sensilla squamiforma, and Bohm’s bristles. Tr.2, Ba.1, and St.1 were only found in females, whereas Ba.2, Ba.3, and St.2 were only observed in males. Sensilla placodea were the most common, given that they occur on the antennae of many parasitoid Hymenoptera, whereas sensilla Tr were the most abundant, being distributed over the entire antennal surface. These sensilla are likely to have roles in the host locating and habitat searching behavior of adult Sclerodermus wasps. Therefore, our findings provide a basis for further studies of the host location behavior of this and other species of parasitic wasp. PMID:25843589

  9. Floral ecology and insect visitation in riparian Tamarix sp. (saltcedar)

    USGS Publications Warehouse

    Andersen, D.C.; Nelson, S.M.

    2013-01-01

    Climate change projections for semiarid and arid North America include reductions in stream discharge that could adversely affect riparian plant species dependent on stream-derived ground water. In order to better understand this potential impact, we used a space-for-time substitution to test the hypotheses that increasing depth-to-groundwater (DGW) is inversely related to Tamarix sp. (saltcedar) flower abundance (F) and nectar production per flower (N). We also assessed whether DGW affected the richness or abundance of insects visiting flowers. We examined Tamarix floral attributes and insect visitation patterns during 2010 and 2011 at three locations along a deep DWG gradient (3.2–4.1 m) on a floodplain terrace adjacent to Las Vegas Wash, an effluent-dominated Mojave Desert stream. Flower abundance and insect visitation patterns differed between years, but no effect from DGW on either F or N was detected. An eruption of a novel non-native herbivore, the splendid tamarisk weevil (Coniatus splendidulus), likely reduced flower production in 2011.

  10. Multiphase complete exchange on Paragon, SP2 and CS-2

    NASA Technical Reports Server (NTRS)

    Bokhari, Shahid H.

    1995-01-01

    The overhead of interprocessor communication is a major factor in limiting the performance of parallel computer systems. The complete exchange is the severest communication pattern in that it requires each processor to send a distinct message to every other processor. This pattern is at the heart of many important parallel applications. On hypercubes, multiphase complete exchange has been developed and shown to provide optimal performance over varying message sizes. Most commercial multicomputer systems do not have a hypercube interconnect. However, they use special purpose hardware and dedicated communication processors to achieve very high performance communication and can be made to emulate the hypercube quite well. Multiphase complete exchange has been implemented on three contemporary parallel architectures: the Intel Paragon, IBM SP2 and Meiko CS-2. The essential features of these machines are described and their basic interprocessor communication overheads are discussed. The performance of multiphase complete exchange is evaluated on each machine. It is shown that the theoretical ideas developed for hypercubes are also applicable in practice to these machines and that multiphase complete exchange can lead to major savings in execution time over traditional solutions.

  11. Streptomyces ziwulingensis sp. nov., isolated from grassland soil.

    PubMed

    Lin, Yan Bing; Wang, Xin Ye; Wang, Ting Ting; An, Shao Shan; Shi, Peng; Wei, Ge Hong

    2013-04-01

    A novel actinobacterium, designated strain F22(T), was isolated from grassland soil collected from the Ziwuling area on the Loess Plateau, China. The novel strain was found to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F22(T) belonged to the genus Streptomyces, being most closely related to Streptomyces resistomycificus NBRC 12814(T) (98.28?% sequence similarity), Streptomyces ciscaucasicus NBRC 12872(T) (98.14?%), Streptomyces chartreusis NBRC 12753(T) (98.14?%) and Streptomyces canus NRRL B-1989(T) (98.14?%). In DNA-DNA hybridizations and comparisons of morphological and phenotypic data, strain F22(T) could be distinguished from all of its closest phylogenetic relatives. Strain F22(T) exhibited antibacterial and antifungal activity, especially against Staphylococcus aureus, Bacillus subtilis and Cylindrocarpon destructans. Based on the DNA-DNA hybridization data and morphological, phenotypic and phylogenetic evidence, strain F22(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces ziwulingensis sp. nov. is proposed. The type strain is F22(T) (?=?CCNWFX 0001(T)?=?JCM 18081(T)?=?ACCC41875(T)). PMID:22888189

  12. Halorubrum rutilum sp. nov. isolated from a marine solar saltern.

    PubMed

    Yin, Shuai; Wang, Zhao; Xu, Jia-Qi; Xu, Wen-Mei; Yuan, Pan-Pan; Cui, Heng-Lin

    2015-12-01

    A halophilic archaeal strain, YJ-18-S1(T), was isolated from Yangjiang marine solar saltern, Guangxi Province, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-18-S1(T) was able to grow at 20-55 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 2.6 M NaCl), at 0.005-1.0 M MgCl2 (optimum 0.3 MgCl2) and at pH 5.5-8.5 (optimum pH 7.0). The cells were lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The 16S rRNA gene and rpoB' gene of strain YJ-18-S1(T) were phylogenetically related to the corresponding genes of Halorubrum members (94.3-98.0 and 86.7-96.1 % similarities, respectively). The DNA G+C content of strain YJ-18-S1(T) was 66.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-18-S1(T) (=CGMCC 1.12554(T) = JCM 30030(T)) represents a new species of Halorubrum, for which the name Halorubrum rutilum sp. nov. is proposed. PMID:26438378

  13. Micromonospora vulcania sp. nov., isolated from volcanic sediment.

    PubMed

    Jia, Feiyu; Liu, Chongxi; Zhou, Shuyu; Li, Jiansong; Shen, Yue; Guan, Xuejiao; Guo, Siyu; Gao, Meiyue; Wang, Xiangjing; Xiang, Wensheng

    2015-12-01

    A novel actinobacterial strain, designated strain NEAU-JM2(T), was isolated from volcanic sediment collected from Longwan, Jilin province, north China and characterized using a polyphasic approach. The strain was found to have morphological and chemotaxonomic characteristics typical of the members of the genus Micromonospora. Phylogenetic analysis of the16S rRNA gene sequence also indicated that strain NEAU-JM2(T) should be classified in the genus Micromonospora and showed that close relatives are Micromonospora maoerensis NEAU-MES19(T) (99.5 %) and Micromonospora matsumotoense JCM 9104(T) (98.8 %). However, phylogenetic analysis based on the gyrB gene sequence showed that the isolate forms a separate subclade away from the close relatives in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. The low level of DNA-DNA relatedness allowed the isolate to be differentiated from M. maoerensis NEAU-MES19(T) and M. matsumotoense JCM 9104(T). Furthermore, strain NEAU-JM2(T) could also be distinguished from its close phylogenetic relatives by cultural and physiological characteristics. Therefore, it is proposed that strain NEAU-JM2(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora vulcania sp. nov. is proposed. The type strain is NEAU-JM2(T) (=CGMCC 4.7144(T) = DSM 46711(T)). PMID:26404428

  14. Sphingomonas arantia sp. nov., isolated from Hoh Xil basin, China.

    PubMed

    Jia, Li; Zheng, Zhong; Feng, Xiaomin; Nogi, Yuichi; Yang, Aichen; Zhang, Yali; Han, Lu; Lu, Zhenquan; Lv, Jie

    2015-12-01

    A Gram-negative, rod-shaped, non-motile, non-spore forming, aerobic, orange-pigmented bacterium, designated strain 6P(T), was isolated from a soil sample collected from the Hoh Xil basin, China. Strain 6P(T) grew optimally at 25 °C, pH 7.0-7.5 and NaCl concentration of 0-1 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 6P(T) belongs to the genus Sphingomonas, with high sequence similarity (97.1 %) to Sphingomonas fennica. The DNA-DNA hybridization homology with S. fennica DSM 13665(T) was 45.3 %. The DNA G+C content of the novel strain is 65.3 mol%. The isolate contained Q-10 as the only respiratory quinone. The major polar lipids are diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC) and sphingoglycolipid (SGL). C18:1 ?7c and C16:1 ?7c are the major fatty acids. On the basis of the polyphasic evidence presented, strain 6P(T) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas arantia sp. nov. is proposed. The type strain is 6P(T) (=CGMCC 1.12702(T) = JCM 19855(T)). PMID:26363912

  15. Biodegradation of pyrazosulfuron-ethyl by Acinetobacter sp. CW17.

    PubMed

    Wang, Yanhui; Du, Liangwei; Chen, Yingxi; Liu, Xiaoliang; Zhou, Xiaomao; Tan, Huihua; Bai, Lianyang; Zeng, Dongqiang

    2012-03-01

    The pyrazosulfuron-ethyl-degrading bacterium, designated as CW17, was isolated from contaminated soil near the warehouse of the factory producing pyrazosulfuron-ethyl in Changsha city, China. The strain CW17 was identified as Acinetobacter sp. based on analyses of 94 carbon source utilization or chemical sensitivity in Biolog microplates, conventional phenotypic characteristics, and 16S rRNA gene sequencing. When pyrazosulfuron-ethyl was provided as the sole carbon source, the effects of pyrazosulfuron-ethyl concentration, pH, and temperature on biodegradation were examined. The degradation rates of pyrazosulfuron-ethyl at initial concentrations of 5.0, 20.0, and 50.0 mg/L were 48.0%, 77.0%, and 32.6%, respectively, after inoculation for 7 days. The growth of the strain was inhibited at low pH buffers. The chemical degradation occurs much faster at low pH than at neutral and basic pH conditions. The degradation rate of pyrazosulfuron-ethyl at 30°C was faster than those at 20 and 37°C by CW17 strains. Two metabolites of degradation were analyzed by liquid chromatography-mass spectroscopy (LC/MS). Based on the identified products, strain CW17 seemed to be able to degrade pyrazosulfuron-ethyl by cleavage of the sulfonylurea bridge. PMID:22388979

  16. Mechanism of algal aggregation by Bacillus sp. strain RP1137.

    PubMed

    Powell, Ryan J; Hill, Russell T

    2014-07-01

    Alga-derived biofuels are one of the best alternatives for economically replacing liquid fossil fuels with a fungible renewable energy source. Production of fuel from algae is technically feasible but not yet economically viable. Harvest of dilute algal biomass from the surrounding water remains one of the largest barriers to economic production of algal biofuel. We identified Bacillus sp. strain RP1137 in a previous study and showed that this strain can rapidly aggregate several biofuel-producing algae in a pH- and divalent-cation-dependent manner. In this study, we further characterized the mechanism of algal aggregation by RP1137. We show that aggregation of both algae and bacteria is optimal in the exponential phase of growth and that the density of ionizable residues on the RP1137 cell surface changes with growth stage. Aggregation likely occurs via charge neutralization with calcium ions at the cell surface of both algae and bacteria. We show that charge neutralization occurs at least in part through binding of calcium to negatively charged teichoic acid residues. The addition of calcium also renders both algae and bacteria more able to bind to hydrophobic beads, suggesting that aggregation may occur through hydrophobic interactions. Knowledge of the aggregation mechanism may enable engineering of RP1137 to obtain more efficient algal harvesting. PMID:24771029

  17. Kennel Disinfectants for Microsporum canis and Trichophyton sp.

    PubMed Central

    Moriello, Karen A.

    2015-01-01

    The antifungal efficacy of commonly used kennel disinfectants for large surfaces was tested using naturally infective material from untreated animals (M. canis and Trichophyton sp.) soaked and macerated but unfiltered leaving visible fluorescing hairs and/or scales in the test inoculum to create a robust challenge. Disinfectants included sodium hypochlorite (1?:?32 and 1?:?100), enilconazole (1?:?100), accelerated hydrogen peroxide (1?:?16), potassium peroxymonosulfate (1% and 2%), and calcium hypochlorite “dry bleach.” Disinfectants were tested at a 1?:?10, 1?:?5, and 1?:?1 dilution of test inoculum to disinfectant with a 10?min contact time. Good efficacy was defined as a disinfectant resulting in no growth. Control plates grew >300 colonies of each pathogen per plate. Enilconazole, sodium hypochlorite (all dilutions), accelerated hydrogen peroxide, and 2% potassium peroxymonosulfate (but not 1%) inhibited all growth of both pathogens at 1?:?10, 1?:?5, and 1?:?1 dilutions. Calcium hypochlorite showed no antifungal efficacy (>300 colonies per plate). Enilconazole (1?:?100), sodium hypochlorite (1?:?32 or 1?:?100), accelerated hydrogen peroxide (1?:?16), and 2% potassium peroxymonosulfate are recommended for decontamination of kennels exposed to dermatophyte pathogens. PMID:25763290

  18. The complete mitochondrial genome of Dixella sp. (Diptera: Nematocera, Dixidae).

    PubMed

    Kang, Zehui; Li, Xuankun; Yang, Ding

    2016-03-01

    In the present paper, the first complete mitochondrial genome of the family Dixidae is reported. The complete mitochondrial genome of Dixella sp. is a circular molecule of 15,574?bp in length, containing all 37 genes including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (srRNA and lrRNA), and a long control region. Its gene arrangement is conserved with the ancestral gene order of Drosophila yakuba, which is considered to exhibit the ground pattern of Hexapoda mitochondrial genome. Most PCGs start with standard ATN codons, while COI uses CCG, ND1 uses TTG and ND5 uses GTG as start codons. All PCGs terminate in the common stop codons TAA, except for COII and ND5 which end with a single thymine stop codon. There is a 703?bp of the control region, located between srRNA and tRNA(lle)-tRNA(Gln)-tRNA(Met) (IQM) cluster, without conserved blocks or long tandem repeats. PMID:25187169

  19. Bacillus huizhouensis sp. nov., isolated from a paddy field soil.

    PubMed

    Li, Jibing; Yang, Guiqin; Wu, Min; Zhao, Yong; Zhou, Shungui

    2014-08-01

    A Gram-stain positive, facultative aerobic bacterium, designated as strain GSS03(T), was isolated from a paddy field soil. The cells were observed to be endospore forming, rod-shaped and motile with flagella. The organism was found to grow optimally at 35 °C at pH 7.0 and in the presence of 1 % NaCl. The strain was classified as a novel taxon within the genus Bacillus on the basis of phenotypic and phylogenetic analyses. The closest phylogenetic relatives were identified as Bacillus psychrosaccharolyticus DSM 6(T) (97.61 %), Bacillus muralis DSM 16288(T) (97.55 %), Bacillus asahii JCM 12112(T) (97.48 %), Bacillus simplex DSM 1321(T) (97.48 %) and "Bacillus frigoritolerans" DSM 8801(T) (97.38 %). The menaquinone was identified as MK-7, the major cellular fatty acid was identified as anteiso-C15:0 and the major cellular polar lipids as phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and three unknown polar lipids. The DNA G+C content was determined to be 40.2 mol%. The DNA-DNA relatedness with the closest relatives was below 48 %. Therefore, on the basis of all the results, strain GSS03(T) is considered to represent a novel species within the genus Bacillus, for which the name Bacillus huizhouensis sp. nov. is proposed. The type strain is GSS03(T) (=KCTC 33172(T) =CCTCC AB 2013237(T)). PMID:24903955

  20. Anthranilate degradation by a cold-adapted Pseudomonas sp.

    PubMed

    Kim, Dockyu; Yoo, Miyoun; Kim, Eungbin; Hong, Soon Gyu

    2015-03-01

    An alpine soil bacterium Pseudomonas sp. strain PAMC 25931 was characterized as eurypsychrophilic (both psychrophilic and mesotolerant) with a broad temperature range of 5-30?°C both for anthranilate (2-aminobenzoate) degradation and concomitant cell growth. Two degradative gene clusters (antABC and catBCA) were detected from a fosmid clone in the PAMC 25931 genomic library; each cluster was confirmed to be specifically induced by anthranilate. When expressed in Escherichia coli, the recombinant AntABC (anthranilate 1,2-dioxygenase, AntDO) converted anthranilate into catechol, exhibiting strict specificity toward anthranilate. Recombinant CatA (catechol 1,2-dioxygenase, C12O) from the organism was active over a broad temperature range (5-37?°C). However, CatA rapidly lost the enzyme activity when incubated at above 25?°C. For example, 1?h-preincubation at 37?°C resulted in 100% loss of enzyme activity, while a counterpart from mesophilic Pseudomonas putida mt-2 did not show any negative effect on the initial enzyme activity. These results suggest that CatA is a new cold-adapted thermolabile enzyme, which might be a product through the adaptation process of PAMC 25931 to naturally cold environments and contribute to its ability to grow on anthranilate there. PMID:23720227

  1. Phylogeny of Rosellinia capetribulensis sp. nov. and its allies (Xylariaceae).

    PubMed

    Bahl, J; Jeewon, R; Hyde, K D

    2005-01-01

    A new Rosellinia species, R. capetribulensis isolated from Calamus sp. in Australia is described. R. capetribulensis is characterized by perithecia immersed within a carbonaceous stroma surrounded by subiculum-like hyphae, asci with large, barrel-shaped amyloid apical apparatus and large dark brown spores. Morphologically, R. capetribulensis appears to be similar to R. bunodes, R. markhamiae and R. megalospora. To gain further insights into the phylogeny of this new taxon we analyzed the ITS-5.8S rDNA using maximum parsimony and likelihood methods. In addition, a morphological dataset also was analyzed phylogenetically to investigate possible affinities. ITS rDNA based phylogenies reveal that R. capetribulensis is closely related to other Rosellinia species showing closest affinity to R. arcuata, RL necatrix and R. pepo. However, analysis of R. capetribulensis forms an unsupported branch sister to these taxa. Results from the morphological matrix indicate a close morphological affinity to members of Rosellinia subgenus Rosellinia. Despite that ITS rDNA and morphological analyses present difficulties in constructing a proper phylogenetic framework among Rosellinia and allied genera, there is sufficient evidence to support the establishment of the new taxon in the genus Rosellinia. The morphological similarities and differences between R. capetribulensis and allied genera such as Astrocystis and Entoleuca are also briefly discussed. PMID:16596960

  2. Styrene oxide isomerase of Sphingopyxis sp. Kp5.2.

    PubMed

    Oelschlägel, Michel; Zimmerling, Juliane; Schlömann, Michael; Tischler, Dirk

    2014-11-01

    Styrene oxide isomerase (SOI) catalyses the isomerization of styrene oxide to phenylacetaldehyde. The enzyme is involved in the aerobic styrene catabolism via side-chain oxidation and allows the biotechnological production of flavours. Here, we reported the isolation of new styrene-degrading bacteria that allowed us to identify novel SOIs. Out of an initial pool of 87 strains potentially utilizing styrene as the sole carbon source, just 14 were found to possess SOI activity. Selected strains were classified phylogenetically based on 16S rRNA genes, screened for SOI genes and styrene-catabolic gene clusters, as well as assayed for SOI production and activity. Genome sequencing allowed bioinformatic analysis of several SOI gene clusters. The isolate Sphingopyxis sp. Kp5.2 was most interesting in that regard because to our knowledge this is the first time it was shown that a member of the family Sphingomonadaceae utilized styrene as the sole carbon source by side-chain oxidation. The corresponding SOI showed a considerable activity of 3.1 U (mg protein)(-1). Most importantly, a higher resistance toward product inhibition in comparison with other SOIs was determined. A phylogenetic analysis of SOIs allowed classification of these biocatalysts from various bacteria and showed the exceptional position of SOI from strain Kp5.2. PMID:25187627

  3. Algibacter amylolyticus sp. nov., isolated from intertidal sediment.

    PubMed

    Zhang, De-Chao; Wu, Jiang; Neuner, Kathrin; Yao, Jianting; Margesin, Rosa

    2015-05-01

    A Gram-reaction-negative, rod-shaped, yellow-pigmented, motile by gliding bacterial strain, designated RU-4-M-4(T), was isolated from intertidal sediment of Sakhalin Island in Russia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RU-4-M-4(T) was related to the genus Algibacter and had highest 16S rRNA gene sequence similarity with Algibacter pectinivorans KACC 14153(T) (97.2%). The major cellular fatty acids were iso-C15 : 0 3-OH, C15: 0 and iso-C15 : 1 G. The predominant menaquinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. The genomic DNA G+C content of strain RU-4-M-4(T) was 36.4 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain RU-4-M-4(T) is a representative of a novel species of the genus Algibacter , for which we propose the name Algibacter amylolyticus sp. nov. (type strain RU-4-M-4(T)?=LMG 28383(T)?=DSM 29199(T)). PMID:25713044

  4. Microbacterium proteolyticum sp. nov. isolated from roots of Halimione portulacoides.

    PubMed

    Alves, Artur; Riesco, Raúl; Correia, António; Trujillo, Martha E

    2015-06-01

    An endophytic actinobacterial strain RZ36T, isolated from roots of the salt-marsh plant Halimione portulacoides, was subjected to a polyphasic taxonomic characterization. 16S rRNA gene sequence analysis revealed that this strain belonged to the genus Microbacterium. The closest phylogenetic relative was Microbacterium hominis DSM 12509T, with a pairwise 16S rRNA gene sequence similarity of 98.8 %. The DNA-DNA hybridization value between strain RZ36T and M. hominis DSM 12509T was 16 %. The affiliation to the genus Microbacterium was corroborated by phenotypic and chemotaxonomic characteristics. The cell-wall peptidoglycan type was B2? and the diagnostic diamino acid was ornithine. Whole-cell sugars detected were galactose, glucose, rhamnose, ribose and xylose. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0 and the major menaquinone was MK-11 (64 %). Main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The genomic DNA G+C content was 69.7 mol%. Thus, on the basis of phenotypic, genotypic and chemotaxonomic data, strain RZ36T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium proteolyticum sp. nov. (type strain RZ36T = DSM 27100T = CECT 8356T) is proposed. PMID:25744585

  5. Nesterenkonia populi sp. nov., an actinobacterium isolated from Populus euphratica.

    PubMed

    Liu, Jia-Meng; Tuo, Li; Habden, Xugela; Guo, Lin; Jiang, Zhong-Ke; Liu, Xian-Fu; Chen, Li; Zhang, Yu-Qin; Sun, Cheng-Hang

    2015-05-01

    An alkaliphilic and moderately halophilic actinobacterium, designated strain GP10-3(T), was isolated from Populus euphratica collected from the southern edge of Taklimakan desert, Xinjiang, China. Cells of this strain were Gram-stain-positive, non-motile and non-spore-forming short rods. Strain GP10-3(T) grew optimally at 37 °C on LB agar media in the presence of 5-10% (w/v) NaCl at pH 9.0. The menaquinones were MK-7, MK-8 and MK-9. The major fatty acids (>10%) were anteiso-C17 : 0, anteiso-C15 : 0 and iso-C16 : 0. The peptidoglycan type was variation A4?, L-Lys-L-Glu. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylglycolipid, phosphatidylcholine, phosphatidylinositol, glycolipid and an unidentified phospholipid. The DNA G+C content was 67.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain GP10-3(T) belonged to the genus Nesterenkonia , sharing 94.6-96.9% sequence similarity with the type strains of species within this genus with validly published names. Based on the evidence of the polyphasic taxonomic study, strain GP10-3(T) represents a novel species of the genus Nesterenkonia , for which the name Nesterenkonia populi sp. nov. is proposed. The type strain is GP10-3(T) (?= DSM 27959(T)?= KCTC 29119(T)). PMID:25701851

  6. Pullulanibacillus pueri sp. nov., isolated from Pu'er tea.

    PubMed

    Niu, Lili; Tang, Tianyi; Song, Lei; Xiong, Mengjie; Tian, Jianqing; Zhang, Kegui; Hu, Xing; Zhu, Daochen

    2015-07-01

    A novel Gram-stain-positive, aerobic, endospore-forming, rod-shaped bacterial strain YN3(T) was isolated from ripened Pu'er tea. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the family Sporolactobacillaceae and was closely related to Pullulanibacillus naganoensis DSM 10191(T) (95.8% 16S rRNA gene sequence similarity) and Pullulanibacillus uraniitolerans DSM 19429(T) (95.4%). Growth of the strain was observed at 20-50 °C (optimum 30-37 °C), at pH 4.0-8.0 (optimum pH 5.0-6.0). The strain had a cell-wall type A1? peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was menaquinone-7 (MK-7). The major fatty acids were anteiso-C15:0, anteiso-C17:0 and C18:1?7c. The DNA G+C content of strain YN3(T) was 38.7 mol%. Strain YN3(T) could be differentiated from recognized species of the genus Pullulanibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of polyphasic evidence from this study, Pullulanibacilluspueri sp. nov., is proposed, with strain YN3(T) (?= CGMCC 1.12777(T ) = JCM 30075(T)) as the type strain. PMID:25858244

  7. Tumebacillus algifaecis sp. nov., isolated from decomposing algal scum.

    PubMed

    Wu, Yu-Fan; Zhang, Bo; Xing, Peng; Wu, Qing-Long; Liu, Shuang-Jiang

    2015-07-01

    Bacterial strain THMBR28(T) was isolated from decomposing algal scum that was collected during an algal bloom in Taihu lake, China. Cells of strain THMBR28(T) were Gram-staining-positive, facultatively anaerobic and rod-shaped. Growth was observed at 20-45 °C (optimum, 30 °C), at pH 5.0-9.5 (optimum, pH 6.5-7.5), and in the presence of 0-1.0% (w/v) NaCl (optimum, 0.5%). Strain THMBR28(T) contained MK-7 as the major menaquinone and iso-C15 : 0 as the major cellular fatty acid. The polar lipid profile contained phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and six unidentified polar lipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The DNA G+C content was 57.6 mol% (Tm). Phylogenetic analysis of 16S rRNA gene sequences showed that strain THMBR28(T) belonged to the genus Tumebacillus, most closely related to Tumebacillus ginsengisoli DSM 18389(T) (95.0%) and Tumebacillus permanentifrigoris Eur1 9.5(T) (93.4%). Based on phylogenetic and phenotypic characterization, it is concluded that strain THMBR28(T) represents a novel species of the genus Tumebacillus, for which the name Tumebacillus algifaecis sp. nov. is proposed, with THMBR28(T) (?= CGMCC 1.10949(T) = NBRC 108765(T)) as the type strain. PMID:25858243

  8. Marinomonas mangrovi sp. nov., isolated from mangrove sediment.

    PubMed

    Zhang, De-Chao; Margesin, Rosa

    2015-05-01

    A Gram-stain-negative, Na(+)-requiring bacterial strain, designated B20-1(T), was isolated from soil of the root system of mangrove forest. Cells were curved rods and motile by means of a polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B20-1(T) belonged to the genus Marinomonas , sharing highest sequence similarities with Marinomonas rhizomae IVIA-Po-145(T) (97.6%), Marinomonas dokdonensis DSW10-10(T) (97.0%) and Marinomonas foliarum IVIA-Po-155(T) (96.9%). The predominant cellular fatty acids of strain B20-1(T) were C10 : 0 3-OH, C18 : 1?7c, summed feature 3 (C16 : 1?7c and/or iso-C15 : 0 2-OH) and C16 : 0. Phosphatidylethanolamine and phosphatidylglycerol were identified as the predominant phospholipids. The predominant ubiquinone was Q-8. The genomic DNA G+C content of strain B20-1(T) was 46.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Marinomonas mangrovi sp. nov., is proposed with B20-1(T) (?=DSM 28136(T)?=LMG 28077(T)) as the type strain. PMID:25713041

  9. Hymenobacter wooponensis sp. nov., isolated from wetland freshwater.

    PubMed

    Kang, Joo Won; Lee, Ji Hee; Baik, Keun Sik; Lee, Sang Suk; Seong, Chi Nam

    2015-06-01

    A non-motile and rod-shaped bacterium, designated strain WM78T, was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15:0, anteiso-C15:0, C16:1?7c and/or C16:1?6c and iso-C17:1 I and/or anteiso-C17:1 B. The strain contained MK-7 as the major isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine. The genomic DNA G+C content was 62 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WM78T forms evolutionary lineage within the radiation comprising members of the genus Hymenobacter with Hymenobacter gelipurpurascens Txg1T (97.7 % sequence similarity) as its nearest neighbour. A number of phenotypic characteristics distinguished strain WM78T from related members of the genus Hymenobacter. The evidence presented in this study support the designation of strain WM78T as a representative of a novel species in the genus Hymenobacter for which the name Hymenbactor wooponensis sp. nov. is proposed. The type strain is WM78T (?= KCTC 32528T = JCM 19491T). PMID:25766109

  10. Discovery of new hazimycin congeners from Kitasatospora sp. P07101

    PubMed Central

    Koyama, Nobuhiro; Sato, Hirofumi; Tomoda, Hiroshi

    2015-01-01

    In an analytical study of microbial broths, the actinomycete strain Kitasatospora sp. P07101 was found to produce three new congeners, which were designated hazimycins B (1), C (2), and D (3), together with the previously reported hazimycin (renamed hazimycin A (4)). The structures of these hazimycins were examined using various spectroscopic methods including nuclear magnetic resonance (NMR), and the results revealed that 1–3 were analogues of hazimycin with the replacement of one of the two isonitrile groups in 4 by an NH-formyl group in 1, the two isonitrile groups and an amide group by two NH-formyl groups and a nitrile group in 2, and the two isonitrile groups and two amide groups by two NH-formyl groups and two nitrile groups in 3. Only hazimycin A exhibited moderate antimicrobial activities against Gram-positive bacteria and Candida albicans. These results indicated that the presence of two isonitrile groups in the hazimycin structure is essential for antimicrobial activity. PMID:26713271

  11. Rhodococcus aerolatus sp. nov., isolated from subarctic rainwater.

    PubMed

    Hwang, C Y; Lee, I; Cho, Y; Lee, Y M; Baek, K; Jung, Y-J; Yang, Y Y; Lee, T; Rhee, T S; Lee, H K

    2015-02-01

    A Gram-stain-positive, rod-shaped and non-motile strain, designated PAMC 27367(T), was isolated from rainwater collected on the Bering Sea. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Rhodococcus. Phylogenetic analyses revealed that strain PAMC 27367(T) formed a robust clade with the type strains of Rhodococcus rhodnii, Rhodococcus aetherivorans and Rhodococcus ruber with 16S rRNA gene sequence similarities of 96.3 %, 95.8 % and 95.5 %, respectively. Cells of the strain grew optimally at 25 °C and at pH 6.5-7.0 in the presence of 0-2 % (w/v) sea salts. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and three unknown phospholipids. The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1?8c and 10-methyl C17 : 0. Cell wall analysis showed that strain PAMC 27367(T) contained meso-diaminopimelic acid. The genomic DNA G+C content was 77.1 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the name Rhodococcus aerolatus sp. nov., with PAMC 27367(T) (?= KCTC 29240(T)?= JCM 19485(T)) as the type strain. PMID:25385992

  12. Streptopyridines, volatile pyridine alkaloids produced by Streptomyces sp. FORM5

    PubMed Central

    Groenhagen, Ulrike; Maczka, Michael; Dickschat, Jeroen S

    2014-01-01

    Summary Streptomyces sp. FORM5 is a bacterium that is known to produce the antibiotic streptazolin and related compounds. We investigated the strain for the production of volatiles using the CLSA (closed-loop stripping analysis) method. Liquid and agar plate cultures revealed the formation of new 2-alkylpyridines (streptopyridines), structurally closely related to the already known 2-pentadienylpiperidines. The structures of the streptopyridines A to E were confirmed by total synthesis. The analysis of the liquid phase by solvent extraction or extraction with an Oasis adsorbent showed that streptazolin and 2-pentadienylpiperidine are the major compounds, while the streptopyridines are only minor components. In the gas phase, only the streptopyridines could be detected. Therefore, an orthogonal set of analysis is needed to assess the metabolic profile of bacteria, because volatile compounds are obviously overlooked by traditional analytical methods. The streptopyridines are strain specific volatiles that are accompanied by a broad range of headspace constituents that occur in many actinomycetes. Volatiles might be of ecological importance for the producing organism, and, as biosynthetic intermediates or shunt products, they can be useful as indicators of antibiotic production in a bacterium. PMID:24991297

  13. Naphthalene removal from aqueous systems by Sagittarius sp..

    PubMed

    Maillacheruvu, Krishnanand; Safaai, Sadaf

    2002-01-01

    Naphthalene is a polyaromatic hydrocarbon (PAH) present in many sediment-water systems. The uptake of naphthalene by an aquatic plant, arrowhead (Sagittaria sp.), was studied in a series of batch systems and continuous flow systems. Batch experiments were conducted in a fed-batch mode, and naphthalene was administered in consecutive spike doses. Results from batch experiments indicated that the rate of naphthalene removal by arrowhead in the presence of light was 0.114+/-0.061 h(-1), at a 90% confidence interval. Naphthalene removal by arrowhead occurred even in the absence of light, although this rate was about 25% of that in the presence of light. Results indicated that repeated exposures to naphthalene for a single arrowhead plant may decrease its capacity for naphthalene uptake, particularly after the first exposure. Naphthalene tended to deposit out in CSTRs where no arrowhead was present, indicating that while the naphthalene may not have been present in the water column, it was not removed from the system. Results from CSTR experiments indicated that under well-mixed quasi-steady state conditions, naphthalene removal by arrowhead in the presence of light was about 40%. Photolytic degradation of naphthalene in the absence of arrowhead was about 15%, when additional mixing was provided to simulate scouring. PMID:12049120

  14. Free amino acids in metamorphosing bonefish (Albula sp.) leptocephali.

    PubMed

    Pfeiler, E

    1987-07-01

    Metamorphosing leptocephalous larvae of the bonefish (Albula sp.) were analyzed for total ninhydrinpositive substances (NPS) and free amino acids. Total NPS content showed little change during metamorphosis. The average NPS value (±S.E.) for 16 larvae was 1.8 (±0.1) mg×larva(-1), which represents approximately 4% and 8% of the total dry weight of early and advanced larvae, respectively. Taurine was the most abundant free amino acid in whole-larva extracts, accounting for 36% and 59% of the total by weight in early and advanced larvae, respectively. The essential amino acids, leucine, isoleucine, phenylalanine, histidine, valine, methionine, lysine and arginine, accounted for about half (47%) of the total in early larvae but were reduced to about 23% of the total in advanced larvae. All of the component essential amino acids decreased during metamorphosis, but the greatest effect was seen with the first five. The remaining non-essential amino acids comprised less than 20% of the total in early larvae and, although the overall value changed little during metamorphosis, certain components such as glycine and glutamic acid showed large increases whereas others such as tyrosine and serine were reduced. Increases in amino acid content after acid hydrolysis of whole-larva extracts indicated that trichloroacetic acid-soluble, low molecular weight peptides were present in both early and advanced leptocephali. PMID:24226035

  15. Efficient Production of Lumichrome by Microbacterium sp. Strain TPU 3598.

    PubMed

    Yamamoto, Kazunori; Asano, Yasuhisa

    2015-11-01

    Lumichrome is a photodegradation product of riboflavin and is available as a photosensitizer and fluorescent dye. To develop new efficient methods of lumichrome production, we isolated bacterial strains with high lumichrome productivity from soil. The strain with highest productivity was identified as Microbacterium sp. strain TPU 3598. Since this strain inductively produced lumichrome when cultivated with riboflavin, we developed two different methods, a cultivation method and a resting cell method, for the production of large amounts of lumichrome using the strain. In the cultivation method, 2.4 g (9.9 mmol) of lumichrome was produced from 3.8 g (10.1 mmol) of riboflavin at the 500-ml scale (98% yield). The strain also produced 4.7 g (19.4 mmol) of lumichrome from 7.6 g (20.2 mmol) of riboflavin (96% yield) by addition of riboflavin during cultivation at the 500-ml scale. In the resting cell method, 20 g of cells (wet weight) in 100 ml of potassium phosphate buffer, pH 7.0, produced 2.4 g of lumichrome from 3.8 g of riboflavin (98% yield). Since the lumichrome production by these methods was carried out in suspension, the resulting lumichrome was easily purified from the cultivation medium or reaction mixture by centrifugation and crystallization. Thus, the biochemical methods we describe here are a significant improvement in terms of simplicity and yield over the existing chemical, photolytic, and other biochemical methods of lumichrome production. PMID:26253661

  16. Efficient Production of Lumichrome by Microbacterium sp. Strain TPU 3598

    PubMed Central

    Yamamoto, Kazunori

    2015-01-01

    Lumichrome is a photodegradation product of riboflavin and is available as a photosensitizer and fluorescent dye. To develop new efficient methods of lumichrome production, we isolated bacterial strains with high lumichrome productivity from soil. The strain with highest productivity was identified as Microbacterium sp. strain TPU 3598. Since this strain inductively produced lumichrome when cultivated with riboflavin, we developed two different methods, a cultivation method and a resting cell method, for the production of large amounts of lumichrome using the strain. In the cultivation method, 2.4 g (9.9 mmol) of lumichrome was produced from 3.8 g (10.1 mmol) of riboflavin at the 500-ml scale (98% yield). The strain also produced 4.7 g (19.4 mmol) of lumichrome from 7.6 g (20.2 mmol) of riboflavin (96% yield) by addition of riboflavin during cultivation at the 500-ml scale. In the resting cell method, 20 g of cells (wet weight) in 100 ml of potassium phosphate buffer, pH 7.0, produced 2.4 g of lumichrome from 3.8 g of riboflavin (98% yield). Since the lumichrome production by these methods was carried out in suspension, the resulting lumichrome was easily purified from the cultivation medium or reaction mixture by centrifugation and crystallization. Thus, the biochemical methods we describe here are a significant improvement in terms of simplicity and yield over the existing chemical, photolytic, and other biochemical methods of lumichrome production. PMID:26253661

  17. Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil.

    PubMed

    Ghosh, A; Bhardwaj, M; Satyanarayana, T; Khurana, M; Mayilraj, S; Jain, R K

    2007-02-01

    A Gram-positive, endospore-forming, alkalitolerant bacterial strain, designated MLB2T, was isolated from soil from Leh, India, and was subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Growth was observed at pH 7.0-11.0, but not at pH 6.0. The DNA G+C content was 41.4 mol%. The highest level of 16S rRNA gene sequence similarity was with Bacillus oshimensis JCM 12663T (98.8 %). However, DNA-DNA hybridization experiments indicated low levels of genomic relatedness with the type strains of B. oshimensis (62 %), Bacillus patagoniensis (55 %), Bacillus clausii (51 %) and Bacillus gibsonii (34 %), the species with which strain MLB2T formed a coherent cluster (based on the results of the phylogenetic analysis). On the basis of the phenotypic characteristics and genotypic distinctiveness of strain MLB2T, it should be classified within a novel species of Bacillus, for which the name Bacillus lehensis sp. nov. is proposed. The type strain is MLB2T (=MTCC 7633T=JCM 13820T). PMID:17267957

  18. The complete mitochondrial genome of Ircinia sp. (Dictyoceratida: Irciniidae).

    PubMed

    Ma, Junye; Li, Chunxiang; Gai, Yonghua; Yang, Qun

    2015-04-01

    The mitochondrial genome of Ircinia sp. (Dictyoceratida: Irciniidae) is a circular molecule of 16,037 bp in length, containing 14 protein-coding genes, 2 ribosomal RNA genes, 2 transfer RNA genes (trnW and trnM) and 13 non-coding segments. All genes are distributed in the same strand (H-strand). The overall base composition of the H-strand is as follows: T (37.84%), C (11.22%), A (24.81%), G (26.13%), with GC- and AT-skew of 0.399 and -0.208, respectively, reflecting unbalanced base composition between the two strands. The non-coding regions are 1190 bp in total length, with high AT content (76.31%). The current mitochondrial genome is identical to that of sibling species I. strobilina in gene order and contents, but differs from the latter in the presence of two kinds of repetitive sequences in the non-coding regions, of which one could form repetitive hairpin-forming elements. PMID:24041447

  19. Ascocotyle (A.) nunezae n. sp. (Digenea: Heterophyidae) from Yucatan, Mexico.

    PubMed

    Scholz, T; Vargas-Vázquez, J; Vidal-Martínez, V M; Aguirre-Macedo, L

    1997-02-01

    A new heterophyid species, Ascocotyle (Ascocotyle) nunezae n. sp., is described from adults found in the intestine of naturally infected heron, Casmerodius albus (type host), from the coastal lagoon of Celestún. Yucatan, Mexico, and a domestic chick (Gallus gallus), experimentally infected with metacercariae from Cichlasoma octofasciatum. The new species is characterized mainly by the number (32-37) and arrangement of circumoral spines, which form I complete row of 25-27 circumoral spines and 6-10 accessory spines on the dorsal side, and by the morphology of the ventrogenital sac with a large gonotyl, consisting of 2 indistinctly separated lobes of vesicular tissue. Ascocotyle (A.) nunezae is placed into the nominotypical subgenus Ascocotyle because of the presence of uterine loops at the pharyngeal region and position of vitelline follicles. However, it differs distinctly from other members of this subgenus by the presence of long intestinal ceca reaching posterior to the ventral sucker. Cichlids of the genus Cichlasoma from cenotes, lakes, and the river Río Hondo in the Yucatan Peninsula were natural second intermediate hosts of A. (A.) nunezae, with metacercariae encysted on their gills. Cichlasoma meeki (Brind) was the most heavily infected fish host (total prevalence 75%; mean intensity 11 +/- 9). PMID:9057711

  20. Biosorption of Nickel from Industrial Wastewater using Zygnema sp.

    NASA Astrophysics Data System (ADS)

    Sivaprakash, Kanchana; Blessi T. L., Adlin; Madhavan, Jeyanthi

    2015-10-01

    Contamination of water sources with heavy metals is a very important pollution problem in the current scenario. Biosorption is an effective method for the removal of heavy metal ions from wastewaters. In this study, the removal of Nickel(II) ions from electroplating industrial wastewater using biosorbent prepared from fresh water algal biomass Zygnema was investigated under batch mode. The sorption efficiency of nickel on Zygnema sp. was evaluated as a function of time, pH and sorbent dosage. The Nickel(II) uptake was dependent on initial pH with pH 3 being the optimum value. For 100 mg/L initial Nickel(II) concentration, sorption equilibrium was attained at a contact time of 100 min. The sorbent dosage affected the biosorption efficiency and maximum removal of 76.4 % was obtained at a dosage of 7.5 g/L. From the performance studies, algal biosorbent Zygnema is found to be a valuable material for the removal of Nickel from industrial wastewater and a better substitute for the conventional adsorbents.

  1. Kennel Disinfectants for Microsporum canis and Trichophyton sp.

    PubMed

    Moriello, Karen A

    2015-01-01

    The antifungal efficacy of commonly used kennel disinfectants for large surfaces was tested using naturally infective material from untreated animals (M. canis and Trichophyton sp.) soaked and macerated but unfiltered leaving visible fluorescing hairs and/or scales in the test inoculum to create a robust challenge. Disinfectants included sodium hypochlorite (1?:?32 and 1?:?100), enilconazole (1?:?100), accelerated hydrogen peroxide (1?:?16), potassium peroxymonosulfate (1% and 2%), and calcium hypochlorite "dry bleach." Disinfectants were tested at a 1?:?10, 1?:?5, and 1?:?1 dilution of test inoculum to disinfectant with a 10?min contact time. Good efficacy was defined as a disinfectant resulting in no growth. Control plates grew >300 colonies of each pathogen per plate. Enilconazole, sodium hypochlorite (all dilutions), accelerated hydrogen peroxide, and 2% potassium peroxymonosulfate (but not 1%) inhibited all growth of both pathogens at 1?:?10, 1?:?5, and 1?:?1 dilutions. Calcium hypochlorite showed no antifungal efficacy (>300 colonies per plate). Enilconazole (1?:?100), sodium hypochlorite (1?:?32 or 1?:?100), accelerated hydrogen peroxide (1?:?16), and 2% potassium peroxymonosulfate are recommended for decontamination of kennels exposed to dermatophyte pathogens. PMID:25763290

  2. Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium.

    PubMed

    Yuan, Menglong; Zhang, Wei; Dai, Shiming; Wu, Jing; Wang, Yingdian; Tao, Tianshen; Chen, Ming; Lin, Min

    2009-06-01

    A Gram-positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain I-0(T), was isolated from a sand sample of the Gobi desert in Xinjiang Autonomous Region, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this isolate represents a novel member of the genus Deinococcus, with low sequence similarities (<94 %) to recognized Deinococcus species. The major cellular fatty acids were C(16 : 1)omega7c and C(16 : 0). Its polar lipid profile contained several unidentified glycolipids, phosphoglycolipids, phospholipids, pigments and an aminophospholipid. The peptidoglycan type was Orn-Gly(2) (A3beta) and the predominant respiratory quinone was MK-8. The DNA G+C content was 65.4 mol%. DNA-DNA relatedness between strain I-0(T) and Deinococcus radiodurans ACCC 10492(T) was 37 %. The strain was shown to be extremely resistant to gamma radiation (>15 kGy) and UV light (>600 J m(-2)). On the basis of the phylogenetic, chemotaxonomic and phenotypic data presented, strain I-0(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus gobiensis sp. nov. is proposed. The type strain is I-0(T) (=DSM 21396(T) =CGMCC 1.7299(T)). PMID:19502345

  3. Comamonas composti sp. nov., isolated from food waste compost.

    PubMed

    Young, Chiu-Chung; Chou, Jui-Hsing; Arun, A B; Yen, Wen-Shao; Sheu, Shih-Yi; Shen, Fo-Ting; Lai, Wai-An; Rekha, P D; Chen, Wen-Ming

    2008-01-01

    A bacterial strain designated YY287(T), isolated from food waste compost, was investigated by polyphasic taxonomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analyses using the 16S rRNA gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Comamonas; its closest neighbours were the type strains Comamonas testosteroni DSM 50244(T) (96.5%), Comamonas terrigena DSM 7099(T) (95.4%), Comamonas odontotermitis Dant 3-8(T) (95.2%) and Comamonas koreensis KCTC 12005(T) (94.6%). Strain YY287(T) was clearly distinguished from all of these strains using phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics. The major fatty acids were 16:0 (33%), 18:1omega7c (13%) and summed feature 3 (16:1omega7c and/or 15:0 iso 2-OH; 41%). The DNA G+C content of the genomic DNA was 62.8 mol%. It is evident from the genotypic and phenotypic data that strain YY287(T) represents a novel species in the genus Comamonas, for which the name Comamonas composti sp. nov. is proposed. The type strain is YY287(T) (=BCRC 17659(T)=LMG 24008(T)). PMID:18175717

  4. Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil.

    PubMed

    Luo, Xuesong; Zhang, Lei; Dai, Jun; Liu, Ming; Zhang, Kundi; An, Hongli; Fang, Chengxiang

    2009-06-01

    Strain XM-003(T) was isolated from a soil sample that originated from the Ximo region in Tibet. Cells of strain XM-003(T) were Gram-negative-staining, non-motile, irregular-shaped rods and the strain grew optimally at 28 degrees C and grew at pH 5-8. It contained MK-7 as the major isoprenoid quinone and iso-C(15 : 0) and C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH as the major fatty acids. Flexirubin-type pigments were absent. The DNA G+C content was 43.4 mol%. The 16S rRNA gene sequence of this strain showed the highest sequence similarity of 96.0 % to Mucilaginibacter kameinonensis SCK(T). A number of physiological biochemical tests and chemotaxonomic markers indicate that strain XM-003(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter ximonensis sp. nov. is proposed. The type strain is XM-003(T) (=CCTCC AB 207094(T) =KCTC 22437(T)). PMID:19502332

  5. Structure of a tetrameric galectin from Cinachyrella sp. (ball sponge)

    SciTech Connect

    Freymann, Douglas M.; Nakamura, Yuka; Focia, Pamela J.; Sakai, Ryuichi; Swanson, Geoffrey T.

    2012-09-01

    The structure of a tetrameric sponge galectin suggests a basis for glutamate receptor potentiation. The galectins are a family of proteins that bind with highest affinity to N-acetyllactosamine disaccharides, which are common constituents of asparagine-linked complex glycans. They play important and diverse physiological roles, particularly in the immune system, and are thought to be critical metastatic agents for many types of cancer cells, including gliomas. A recent bioactivity-based screen of marine sponge (Cinachyrella sp.) extract identified an ancestral member of the galectin family based on its unexpected ability to positively modulate mammalian ionotropic glutamate receptor function. To gain insight into the mechanistic basis of this activity, the 2.1 Å resolution X-ray structure of one member of the family, galectin CchG-1, is reported. While the protomer exhibited structural similarity to mammalian prototype galectin, CchG-1 adopts a novel tetrameric arrangement in which a rigid toroidal-shaped ‘donut’ is stabilized in part by the packing of pairs of vicinal disulfide bonds. Twofold symmetry between binding-site pairs provides a basis for a model for interaction with ionotropic glutamate receptors.

  6. Brevundimonas viscosa sp. nov., isolated from saline soil.

    PubMed

    Wang, Jiewei; Zhang, Jianli; Ding, Kai; Xin, Yuhua; Pang, Huancheng

    2012-10-01

    A Gram-negative, rod-shaped bacterial strain, designated F3(T), was isolated from a saline soil sample in China and studied by using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F3(T) was affiliated with the genus Brevundimonas, with Brevundimonas kwangchunensis KSL-102(T) (98.4?% similarity) and Brevundimonas alba DSM 4736(T) (98.2?%) as its closest relatives. Strain F3(T) contained ubiquinone-10 (Q-10) as the predominant ubiquinone and C(18?:?1)?7c, C(17?:?1)?8c and C(16?:?0) as the major fatty acids. The DNA G+C content of strain F3(T) was 66.7 mol%. Levels of DNA-DNA relatedness between strain F3(T) and the type strains of closely related Brevundimonas species were below 22?%. On the basis of phenotypic characteristics and genotypic distinctiveness, strain F3(T) should be classified as representing a novel species of the genus Brevundimonas, for which the name Brevundimonas viscosa sp. nov. is proposed. The type strain is F3(T) (?=?CGMCC 1.10683(T)?=?JCM 17426(T)). PMID:22140155

  7. Thermal hydraulics analysis of LIBRA-SP target chamber

    SciTech Connect

    Mogahed, E.A.

    1996-12-31

    LIBRA-SP is a conceptual design study of an inertially confined 1000 MWe fusion power reactor utilizing self-pinched light ion beams. There are 24 ion beams which are arranged around the reactor cavity. The reaction chamber is an upright cylinder with an inverted conical roof resembling a mushroom, and a pool floor. The vertical sides of the cylinder are occupied by a blanket zone consisting of many perforated rigid HT-9 ferritic steel tubes called PERITs (PEr-forated RIgid Tube). The breeding/cooling material, liquid lead-lithium, flows through the PERITs, providing protection to the reflector/vacuum chamber so as to make it a lifetime component. The neutronics analysis and cavity hydrodynamics calculations are performed to account for the neutron heating and also to determine the effects of vaporization/condensation processes on the surface heat flux. The steady state nuclear heating distribution at the midplane is used for thermal hydraulics calculations. The maximum surface temperature of the HT-9 is chosen to not exceed 625{degree}C to avoid drastic deterioration of the metal`s mechanical properties. This choice restricts the thermal hydraulics performance of the reaction cavity. The inlet first surface coolant bulk temperature is 370{degree}C, and the heat exchanger inlet coolant bulk temperature is 502{degree}C. 4 refs., 6 figs., 2 tabs.

  8. Batrachochytrium salamandrivorans sp. nov. causes lethal chytridiomycosis in amphibians.

    PubMed

    Martel, An; Spitzen-van der Sluijs, Annemarieke; Blooi, Mark; Bert, Wim; Ducatelle, Richard; Fisher, Matthew C; Woeltjes, Antonius; Bosman, Wilbert; Chiers, Koen; Bossuyt, Franky; Pasmans, Frank

    2013-09-17

    The current biodiversity crisis encompasses a sixth mass extinction event affecting the entire class of amphibians. The infectious disease chytridiomycosis is considered one of the major drivers of global amphibian population decline and extinction and is thought to be caused by a single species of aquatic fungus, Batrachochytrium dendrobatidis. However, several amphibian population declines remain unexplained, among them a steep decrease in fire salamander populations (Salamandra salamandra) that has brought this species to the edge of local extinction. Here we isolated and characterized a unique chytrid fungus, Batrachochytrium salamandrivorans sp. nov., from this salamander population. This chytrid causes erosive skin disease and rapid mortality in experimentally infected fire salamanders and was present in skin lesions of salamanders found dead during the decline event. Together with the closely related B. dendrobatidis, this taxon forms a well-supported chytridiomycete clade, adapted to vertebrate hosts and highly pathogenic to amphibians. However, the lower thermal growth preference of B. salamandrivorans, compared with B. dendrobatidis, and resistance of midwife toads (Alytes obstetricans) to experimental infection with B. salamandrivorans suggest differential niche occupation of the two chytrid fungi. PMID:24003137

  9. Bioactive spirobisnaphthalenes from the endophytic fungus Berkleasmium sp.

    PubMed

    Shan, Tijiang; Tian, Jin; Wang, Xiaohan; Mou, Yan; Mao, Ziling; Lai, Daowan; Dai, Jungui; Peng, Youliang; Zhou, Ligang; Wang, Mingan

    2014-10-24

    Nine new spirobisnaphthalenes, palmarumycins B1-B9 (1-9), along with 13 known compounds (10-22), were isolated from cultures of the fungus Berkleasmium sp., an endophyte isolated from the medicinal plant Dioscorea zingiberensis C. H. Wright. The structures of the new compounds were elucidated by analysis of the 1D and 2D NMR and HRESIMS spectra and by comparison with known compounds. Compounds 7-9 contain an uncommon 2,3-dihydro-1H-inden-1-one unit. All isolated compounds were evaluated for their antibacterial activities against Bacillus subtilis, Staphylococcus hemolyticus, Agrobacterium tumefaciens, Pseudomonas lachrymans, Ralstonia solanacearum, and Xanthomonas vesicatoria and for their antifungal effects against the spore germination of Magnaporthe oryzae. Palmarumycin C8 (22) exhibited the best antibacterial and antifungal effects. In addition, diepoxin ? (11) and palmarumycin C8 (22) showed pronounced cytotoxic activities against five human cancer cell lines (HCT-8, Bel-7402, BGC-823, A 549, A 2780) with IC50 values of 1.28-5.83 ?M. PMID:25237727

  10. Subcutaneous Phaeohyphomycotic Nodule Due to Phialemoniopsis hongkongensis sp. nov.

    PubMed Central

    Tsang, Chi-Ching; Chan, Jasper F. W.; Ip, Philip P. C.; Ngan, Antonio H. Y.; Chen, Jonathan H. K.

    2014-01-01

    Phialemoniopsis species are ubiquitous dematiaceous molds associated with a wide variety of superficial and systemic infections in human. In this study, we isolated a mold from the forearm nodule biopsy specimen from a patient with underlying liver cirrhosis, ankylosing spondylosis, and tuberculosis. He was treated with itraconazole, but unfortunately, he succumbed as a result of disseminated tuberculosis with multiorgan failure. The histology results of the skin biopsy showed necrotizing granulomas in which numerous fungal elements were found. On Sabouraud dextrose agar, the fungal isolate grew as white-to-cream and smooth-to-velvety colonies. Microscopically, oval-to-cylindrical conidia were observed from abundant adelophialides, which possessed barely visible parallel collarettes but no basal septa. The azole drugs voriconazole, itraconazole, and posaconazole, as well as amphotericin B, showed high activities against this fungus. Internal transcribed spacer, 28S nuclear ribosomal DNA (nrDNA), and ?-actin and ?-tubulin gene sequencing showed that this fungus is most closely related to but distinct from Phialemonium curvata. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and hierarchical cluster analysis showed that the MALDI-TOF MS spectrum of this fungus is most closely related to that of Phialemonium pluriloculosa. We propose a new species, Phialemoniopsis hongkongensis sp. nov., to describe this fungus. PMID:24966363

  11. Greensporones: Resorcylic Acid Lactones from an Aquatic Halenospora sp.

    PubMed Central

    2015-01-01

    Fourteen new resorcylic acid lactones (1–14) were isolated from an organic extract of a culture of a freshwater aquatic fungus Halenospora sp. originating from a stream in North Carolina. The structures were elucidated using a set of spectroscopic and spectrometric techniques. The absolute configuration of one representative member of the compounds (7) was assigned using X-ray crystallography of an analogue that incorporated a heavy atom, whereas for compounds 8–11, a modified Mosher’s ester method was utilized. The relative configurations of compounds 12–14 were determined on the basis of NOE data. Compounds 12–14 were proposed as artifacts produced by intramolecular cycloetherification of the ?-hydroxy-?,?-unsaturated ketone moieties of the parent compounds during the purification processes. The isolated compounds, except for 8 and 12, were tested against the MDA-MB-435 (melanoma) and HT-29 (colon) cancer cell lines. Compound 5 was the most potent, with IC50 values of 2.9 and 7.5 ?M, respectively. The compounds were evaluated as TAK1–TAB1 inhibitors but were found to be inactive. PMID:25093280

  12. Streptomyces mangrovi sp. nov., isolated from mangrove forest sediment.

    PubMed

    Yousif, Ghada; Busarakam, Kanungnid; Kim, Byung-Yong; Goodfellow, Michael

    2015-09-01

    A Streptomyces strain isolated from a mangrove sediment was classified using a polyphasic approach. The organism, isolate GY1(T), was found to have chemical and morphological properties typical of members of the genus Streptomyces. The isolate was shown to form a distinct phyletic line within the Streptomyces radiopugnans 16S rRNA gene subclade and to be closely related to the type strain of Streptomyces fenhuangensis (98.7 % similarity). It is also closely related to the type strain of Streptomyces bakulensis which was also closely related to members of the Streptomyces glaucosporus 16S rRNA gene subclade. Isolate GY1(T) was distinguished readily from the S. barkulensis type strain and from species classified in the S. radiopugnans clade using a combination of morphological and physiological properties, including a requirement for seawater for growth. Based on the genotypic and phenotypic data, it is proposed that isolate GY1(T) (=NCIMB 14980(T), NRRL B-69296(T)) be classified in the genus Streptomyces as Streptomyces mangrovi sp. nov. PMID:26187116

  13. Cryptosporidium erinacei n. sp. (Apicomplexa: Cryptosporidiidae) in hedgehogs.

    PubMed

    Kvá?, Martin; Hofmannová, Lada; Hlásková, Lenka; Kv?to?ová, Dana; Vítovec, Ji?í; McEvoy, John; Sak, Bohumil

    2014-03-17

    The morphological, biological, and molecular characteristics of Cryptosporidium hedgehog genotype are described, and the species name Cryptosporidium erinacei n. sp. is proposed to reflect its specificity for hedgehogs under natural and experimental conditions. Oocysts of C. erinacei are morphologically indistinguishable from Cryptosporidium parvum, measuring 4.5-5.8 ?m (mean=4.9 ?m) × 4.0-4.8 ?m (mean=4.4 ?m) with a length to width ratio of 1.13 (1.02-1.35) (n=100). Oocysts of C. erinacei obtained from a naturally infected European hedgehog (Erinaceus europaeus) were infectious for naïve 8-week-old four-toed hedgehogs (Atelerix albiventris); the prepatent period was 4-5 days post infection (DPI) and the patent period was longer than 20 days. C. erinacei was not infectious for 8-week-old SCID and BALB/c mice (Mus musculus), Mongolian gerbils (Meriones unguiculatus), or golden hamsters (Mesocricetus auratus). Phylogenetic analyses based on small subunit rRNA, 60 kDa glycoprotein, actin, Cryptosporidium oocyst wall protein, thrombospondin-related adhesive protein of Cryptosporidium-1, and heat shock protein 70 gene sequences revealed that C. erinacei is genetically distinct from previously described Cryptosporidium species. PMID:24529828

  14. An ammonium sulfate sensitive chitinase from Streptomyces sp. CS501.

    PubMed

    Rahman, Md Arifur; Choi, Yun Hee; Pradeep, G C; Yoo, Jin Cheol

    2014-12-01

    A chitinase from Streptomyces sp. CS501 was isolated from the Korean soil sample, purified by single-step chromatography, and biochemically characterized. The extracellular chitinase (Ch501) was purified to 4.60 fold with yield of 28.74 % using Sepharose Cl-6B column. The molecular mass of Ch501 was approximately 43 kDa as estimated by SDS-PAGE and zymography. The enzyme (Ch501) was found to be stable over a broad pH range (5.0-10.0) and temperature (up to 50 °C), and have an optimum temperature of 60 °C. N-terminal sequence of Ch501 was AAYDDAAAAA. Intriguingly, Ch501 was highly sensitive to ammonium sulfate but it's completely suppressed activity was recovered after desalting out. TLC analysis of Ch501 showed the production of N-acetyl D-glucosamine (GlcNAc) and Diacetylchitobiose (GlcNAc)2, as a principal hydrolyzed product. Ch501 shows antifungal activity against Fusarium solani and Aspergillus brasiliensis, which can be used for the biological control of fungus. As has been simple in purification, stable in a broad range of pH, ability to produce oligosaccharides, and antifungal activity showed that Ch501 has potential applications in industries as for chitooligosaccharides production used as prebiotics and/or for the biological control of plant pathogens in agriculture. PMID:25359199

  15. Arcanobacterium phocisimile sp. nov., isolated from harbour seals.

    PubMed

    Hijazin, Muaz; Sammra, Osama; Ulbegi-Mohyla, Hivda; Nagib, Samy; Alber, Jörg; Lämmler, Christoph; Kämpfer, Peter; Glaeser, Stefanie P; Busse, Hans-Jürgen; Kassmannhuber, Johannes; Prenger-Berninghoff, Ellen; Weiss, Reinhard; Siebert, Ursula; Hassan, Abdulwahed Ahmed; Abdulmawjood, Amir; Zschöck, Michael

    2013-06-01

    A polyphasic taxonomic study was performed on two previously unidentified Arcanobacterium-like Gram-positive strains isolated from harbour seals. Comparative 16S rRNA gene sequencing showed that both bacteria belonged to the genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum CIP 103370(T) (98.4% 16S rRNA gene sequence similarity), A. canis P6775(T) (97.4%), A. phocae DSM 10002(T) (97.4%), A. pluranimalium M430/94/2(T) (95.7%) and A. hippocoleae CCUG 44697(T) (95.5%). The presence of the major menaquinone MK-9(H4) supported the affiliation of the isolates with the genus Arcanobacterium. The polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and two unidentified glycolipids. The major fatty acids were C16:0, C18:0, C18:1?9c and summed feature 5 (comprising C18:2?6,9c and/or anteiso-C18:0). Physiological and biochemical tests clearly distinguished the isolates from other members of the genus Arcanobacterium. Based on the common origin and various physiological properties comparable to those of A. phocae, it is proposed that the isolates are classified as members of a novel species with the name Arcanobacterium phocisimile sp. nov. The type strain is 2698(T) (=LMG 27073(T) =CCM 8430(T)). PMID:23041640

  16. Mechanism of Algal Aggregation by Bacillus sp. Strain RP1137

    PubMed Central

    Powell, Ryan J.

    2014-01-01

    Alga-derived biofuels are one of the best alternatives for economically replacing liquid fossil fuels with a fungible renewable energy source. Production of fuel from algae is technically feasible but not yet economically viable. Harvest of dilute algal biomass from the surrounding water remains one of the largest barriers to economic production of algal biofuel. We identified Bacillus sp. strain RP1137 in a previous study and showed that this strain can rapidly aggregate several biofuel-producing algae in a pH- and divalent-cation-dependent manner. In this study, we further characterized the mechanism of algal aggregation by RP1137. We show that aggregation of both algae and bacteria is optimal in the exponential phase of growth and that the density of ionizable residues on the RP1137 cell surface changes with growth stage. Aggregation likely occurs via charge neutralization with calcium ions at the cell surface of both algae and bacteria. We show that charge neutralization occurs at least in part through binding of calcium to negatively charged teichoic acid residues. The addition of calcium also renders both algae and bacteria more able to bind to hydrophobic beads, suggesting that aggregation may occur through hydrophobic interactions. Knowledge of the aggregation mechanism may enable engineering of RP1137 to obtain more efficient algal harvesting. PMID:24771029

  17. SP-100 attitude control pathfinder study. Technical information report

    SciTech Connect

    Eke, F.O.; Graff, S.H.; Laskin, R.A.; Swan, P.A.

    1984-03-01

    This report delineates the scope of Jet Propulsion Laboratory`s FY`83 effort in the attitude control area in support of the SP-100 program. Dynamic modeling of the baseline beam configuration has been conducted and is presented herein. As a first cut, the beam is treated as rigid. Its inherent flexibility is then integrated via the hybrid coordinates method. Using the resulting dynamical equations, a preliminary look at attitude control is taken. Only one axis of rotational one flexible mode are included. An alternative to the beam configuration is one that envisions connecting basebody to user via a long, lightweight, flexible tether. A literature search has been conducted in this area and the resulting bibliography is presented. The tether option is not considered viable near term. However, it offers several potentially significant advantages and thus deserves serious consideration for the next generation space power system. This report also treats attitude control constraints imposed by the high temperature and radiation environment and addresses the issue of hardware requirements and availability. Recommendations for FY`84 tasks include assembling and exercising a simulation program for the beam configuration dynamic model and conducting a technology assessment in the area of tether dynamics and control.

  18. Thiohalocapsa marina sp. nov., from an Indian marine aquaculture pond.

    PubMed

    Anil Kumar, P; Srinivas, T N R; Thiel, V; Tank, M; Sasikala, Ch; Ramana, Ch V; Imhoff, J F

    2009-09-01

    A spherical-shaped, phototrophic, purple sulfur bacterium was isolated in pure culture from anoxic sediment in a marine aquaculture pond near Bheemli (India). Strain JA142T is Gram-negative and non-motile. It has a requirement for NaCl (optimum of 2% and maximum of 6% w/v NaCl). Intracellular photosynthetic membranes are of the vesicular type. In vivo absorption spectra indicate the presence of bacteriochlorophyll a and carotenoids of the okenone series as photosynthetic pigments. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA142T is related to halophilic purple sulfur bacteria of the genera Thiohalocapsa and Halochromatium, with the highest sequence similarity to Thiohalocapsa halophila DSM 6210T (97.5%). Morphological and physiological characteristics differentiate strain JA142T from other species of the genera Halochromatium and Thiohalocapsa. Strain JA142T is sufficiently different from Thiohalocapsa halophila based on 16S rRNA gene sequence analysis and morphological and physiological characteristics to allow the proposal of a novel species, Thiohalocapsa marina sp. nov., with the type strain JA142T (=JCM 14780T=DSM 19078T). PMID:19620368

  19. Lysobacter terrae sp. nov. isolated from Aglaia odorata rhizosphere soil.

    PubMed

    Ngo, Hien T T; Won, KyungHwa; Du, Juan; Son, Heung-Min; Park, YongJin; Kook, MooChang; Kim, Ki-Young; Jin, Feng-Xie; Yi, Tae-Hoo

    2015-02-01

    A Gram-stain negative, facultatively anaerobic, non-motile, rod-shaped bacterium, designated strain THG-A13(T), was isolated from Aglaia odorata rhizosphere soil in Gyeonggi-do, Republic of Korea. Based on 16S rRNA gene sequence comparisons, strain THG-A13(T) had close similarity with Lysobacter niabensis GH34-4(T) (98.5 %), Lysobacter oryzae YC6269(T) (97.9 %) and Lysobacter yangpyeongensis GH19-3(T) (97.3 %). Chemotaxonomic data revealed that strain THG-A13(T) possesses ubiquinone-8 (Q8) as the predominant isoprenoid quinone and iso-C15 : 0, iso-C16 : 0 and iso-C17 : 1?9c as the major fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol) and diphosphatidylglycerol. The G+C content was 66.3 mol%. The DNA-DNA relatedness values between strain THG-A13(T) and its closest phylogenetic neighbours were below 18.0 %. These data corroborated the affiliation of strain THG-A13(T) to the genus Lysobacter. These data suggest that the isolate represents a novel species for which the name Lysobacter terrae sp. nov. is proposed, with THG-A13(T) as the type strain (?=?KACC 17646(T)?= JCM 19613(T)). PMID:25406233

  20. Uruburuella testudinis sp. nov., isolated from tortoise (Testudo).

    PubMed

    Kuhnert, Peter; Thomann, Andreas; Brodard, Isabelle; Haefeli, Willi; Korczak, Bo?ena M

    2015-04-01

    A polyphasic taxonomic analysis was carried out on 11 uncommon Gram-stain-negative, non-motile, catalase- and oxidase-positive, but indole-negative, bacterial strains isolated from tortoises. Phenotypically and genetically they represented a homogeneous group of organisms most closely related to, but distinct from, Uruburuella suis. In a reconstructed 16S rRNA gene tree they clustered on a monophyletic branch next to U. suis with gene similarities between strains of 99.5-100%, and of up to 98.2% with U. suis . DNA-DNA hybridization indicated the organisms represented a novel species with only 40% DNA-DNA similarity with U. suis . Partial sequencing of rpoB resulted in two subclusters confirming the 16S rRNA gene phylogeny; both genes allowed clear separation and identification of the novel species. Furthermore, they could be unambiguously identified by matrix-assisted laser desorption ionization time-of-flight MS, where, again, they formed a highly homogeneous cluster separate from U. suis and other members of the family Neisseriaceae . The major fatty acids were C(16?:?0) and summed feature C(16?:?1)?7c/iso-C(15?:?0) 2-OH. The DNA G+C content was 54.4 mol%. Based on phenotypic and genetic data we propose classifying these organisms as representatives of a novel species named Uruburuella testudinis sp. nov. The type strain is 07_OD624(T) (?=?DSM 26510(T)?=?CCUG 63373(T)). PMID:25634951