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1

COMPARACIÓN DE LA COMPOSICIÓN QUÍMICA DE LOS METABOLITOS SECUNDARIOS VOLÁTILES DE CUATRO PLANTAS DE LA FAMILIA PIPERACEAE OBTENIDOS POR DESTILACIÓN-EXTRACCIÓN SIMULTÁNEA  

Microsoft Academic Search

Volatile fractions were isolated by simultaneous distillation- solvent extraction (SDE) from leaves of four Piperaceae species: Piper marginatum Jacq, P. aduncum L., P. hispidum Sw. and Pothomorphe peltata L. Components were separated and identified by gas chromatography coupled to mass spectrometry (GC - MS).

EDWIN J. BOTTIA; Estudiante de Química; OLGA L. DÍAZ; DEYNY L. MENDIVELSO; JAIRO RENÉ MARTÍNEZ; ELENA E. STASHENKO

2007-01-01

2

Plasmids in Frankia sp.  

PubMed Central

A method to achieve cell lysis and isolate Frankia sp. plasmid DNA was developed. A screening of Frankia sp. strains belonging to different host compatibility groups (Alnus sp., Elaeagnus sp., Ceanothus sp.) showed that, of 39 strains tested, 4 (strains Cp11, ARgN22d, ArI3, and EUN1f) possessed plasmids ranging in size from 7.1 to 32.2 kilobase pairs as estimated from agarose gel electrophoresis and electron microscopy. A total of 11 plasmids were detected. Images

Normand, P; Simonet, P; Butour, J L; Rosenberg, C; Moiroud, A; Lalonde, M

1983-01-01

3

Sp2 ZPP NP  

Microsoft Academic Search

We show that the class Sp2 is contained in ZPP NP . The proof uses universal hashing, approximate counting and witness sampling. As a consequence, a collapse rst noticed by Samik Sengupta that the assumption NP has small circuits collapses PH to Sp2 becomes the strongest version to date of the Karp-Lipton Theorem. We also discuss the problem of nding

Jin-Yi Cai

4

Pseudomonas kuykendallii sp. nov.  

Technology Transfer Automated Retrieval System (TEKTRAN)

This is a submission to the list of microorganisms with standing in nomenclature maintained by the International Journal of Systematic and Evolutionary Microbiology. We wish to have Pseudomonas kuykendallii sp. nov. added to the list as a valid species belonging to the genus Pseudomonas. Three str...

5

The Sp-family of transcription factors  

Microsoft Academic Search

GC-boxes and related motifs are frequently occurring DNA-elements present in many promoters and enhancers. In contrast to other elements it was generally thought that the transcription factor Sp1 is the only factor acting through these motifs. The cloning of paralogous genes of the Sp1 factor uncovered the existence of a small protein family consisting of Sp1, Sp2, Sp3 and Sp4.

G. Suske

1999-01-01

6

Micromonospora mirobrigensis sp. nov.  

PubMed

An actinomycete strain was recovered from a pond where radon is known to be dissolved. A polyphasic study was undertaken to identify the new isolate. The 16S rRNA gene sequence of strain WA201(T) showed closest similarity to the type strains of Micromonospora carbonacea (98.5 %) and Micromonospora matsumotoense (98.1 %). The chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Micromonospora. DNA-DNA relatedness values supported the classification of this isolate as a novel species. A number of physiological and biochemical tests were able to distinguish strain WA201(T) from its closest phylogenetic neighbours. Therefore, it is proposed that isolate WA201(T) (=DSM 44830(T)=LMG 22229(T)) be considered the type strain representing a novel species, Micromonospora mirobrigensis sp. nov. PMID:15774678

Trujillo, Martha E; Fernández-Molinero, Carmen; Velázquez, Encarna; Kroppenstedt, Reiner M; Schumann, Peter; Mateos, Pedro F; Martínez-Molina, Eustoquio

2005-03-01

7

Streptomyces tateyamensis sp. nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the marine sponge Haliclona sp.  

PubMed

Three Gram-positive, NaCl-requiring actinobacteria were isolated from a marine sponge, Haliclona sp., collected from the coast of Tateyama City, Japan. Comparison of 16S rRNA gene sequences indicated that these strains represent novel members of the genus Streptomyces, exhibiting low 16S rRNA gene sequence similarities of 98.3-97.4?% with recognized members of the genus. The cell hydrolysates contained the LL-isomer of diaminopimelic acid and the predominant quinones were MK-9 (H(6) and/or H(8)). The DNA G+C contents were in the range 72-75mol%. A polyphasic study of the strains and comparison of the characters with related species of the genus show that these strains represent three novel species of the genus Streptomyces. Therefore, the names Streptomyces tateyamensis sp. nov., Streptomyces haliclonae sp. nov. and Streptomyces marinus sp. nov. are proposed for strains Sp080513SC-30(T) (=NBRC 105048(T) =DSM 41969(T)), Sp080513SC-31(T) (=NBRC 105049(T) =DSM 41970(T)) and Sp080513GE-26(T) (=NBRC 105047(T) =DSM 41968(T)), respectively. PMID:20061489

Khan, Shams Tabrez; Tamura, Tomohiko; Takagi, Motoki; Shin-Ya, Kazuo

2010-01-08

8

SP-100 space reactor safety  

SciTech Connect

The SP-100 space reactor power system is being developed to meet the large electrical power requirements of civilian and military missions planned for the 1990's and beyond. It will remove the restrictions on electrical power generation that have tended to limit missions and will enable the fuller exploration and utilization of space. This booklet describes the SP-100 space reactor power system and its development. Particular emphasis is given to safety. The design aand operational features as well as the design and safety review process that will assure that the SP-100 can be launched nd operated safely are described.

Not Available

1987-05-01

9

Ovos de Toxocara sp. e larvas de Ancylostoma sp. em praça pública de Lavras, MG Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil  

Microsoft Academic Search

Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both

Antônio Marcos Guimarães; Endrigo Gabellini; Leonel Alves; Glycia Ferreira de Rezende; Marcelo Costa Rodrigues

10

The Sp(1)-Kepler problems  

NASA Astrophysics Data System (ADS)

Let n>=2 be a positive integer. To each irreducible representation ? of Sp(1), an Sp(1)-Kepler problem in dimension (4n-3) is constructed and analyzed. This system is superintegrable, and when n=2 it is equivalent to a generalized MICZ-Kepler problem in dimension of 5. The dynamical symmetry group of this system is O~*(4n) with the Hilbert space of bound states (?) being the unitary highest weight representation of O*~(4n) with highest weight, (-1,...,-1,-(1+?)), which occurs at the rightmost nontrivial reduction point in the Enright-Howe-Wallach classification diagram for the unitary highest weight modules. Here ? is the highest weight of ?. Furthermore, it is shown that the correspondence ?<-->(?) is the theta-correspondence for dual pair (Sp(1),O*(4n))?Sp(8n,R).

Meng, Guowu

2009-07-01

11

Actinoplanes couchii sp. nov.  

PubMed

A Gram-positive bacterium, strain GW8-1761(T), was isolated from soil close to the Marmore waterfalls, Terni, Italy. 16S rRNA gene sequence similarity studies showed that strain GW8-1761(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes italicus JCM 3165(T) (98.9 %), A. rectilineatus IFO 13941(T) (98.5 %), A. palleronii JCM 7626(T) (97.8 %), A. utahensis IFO 13244(T) (97.6 %) and A. cyaneus DSM 46137(T) (97.6 %). Strain GW8-1761(T) could be distinguished from any other Actinoplanes species with validly published names by 16S rRNA gene sequence similarity values of less than 97.5 %. Chemotaxonomic data [major menaquinone MK-9(H(4)); major polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol, with phosphatidylcholine and aminoglycolipids absent; major fatty acids C(15 : 0), C(16 : 0), C(16 : 0) iso, C(17 : 1)omega8c and summed feature 3 (C(16 : 1)omega7c and/or C(15 : 0) iso 2-OH)] supported the affiliation of strain GW8-1761(T) to the genus Actinoplanes. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW8-1761(T) from the most closely related species. Strain GW8-1761(T) therefore merits species status, and we propose the name Actinoplanes couchii sp. nov., with the type strain GW8-1761(T) (=DSM 45050(T)=CIP 109316(T)). PMID:17392194

Kämpfer, Peter; Huber, Birgit; Thummes, Kathrin; Grün-Wollny, Iris; Busse, Hans-Jürgen

2007-04-01

12

Interplay of posttranslational modifications in Sp1 mediates Sp1 stability during cell cycle progression.  

PubMed

Although Sp1 is known to undergo posttranslational modifications such as phosphorylation, glycosylation, acetylation, sumoylation, and ubiquitination, little is known about the possible interplay between the different forms of Sp1 that may affect its overall levels. It is also unknown whether changes in the levels of Sp1 influence any biological cell processes. Here, we identified RNF4 as the ubiquitin E3 ligase of Sp1. From in vitro and in vivo experiments, we found that sumoylated Sp1 can recruit RNF4 as a ubiquitin E3 ligase that subjects sumoylated Sp1 to proteasomal degradation. Sp1 mapping revealed two ubiquitination-related domains: a small ubiquitin-like modifier in the N-terminus of Sp1(Lys16) and the C-terminus of Sp1 that directly interacts with RNF4. Interestingly, when Sp1 was phosphorylated at Thr739 by c-Jun NH(2)-terminal kinase 1 during mitosis, this phosphorylated form of Sp1 abolished the Sp1-RNF4 interaction. Our results show that, while sumoylated Sp1 subjects to proteasomal degradation, the phosphorylation that occurs during the cell cycle can protect Sp1 from degradation by repressing the Sp1-RNF4 interaction. Thus, we propose that the interplay between posttranslational modifications of Sp1 plays an important role in cell cycle progression and keeps Sp1 at a critical level for mitosis. PMID:21983342

Wang, Yi-Ting; Yang, Wen-Bin; Chang, Wen-Chang; Hung, Jan-Jong

2011-09-28

13

SP-100 reactor cell activation  

SciTech Connect

There are plans to test the SP-100 space reactor for 2 yr in the test facility shown in Figure 1. The vacuum vessel will be in the reactor experiment (RX) cell surrounded by an inert gas atmosphere. It is proposed that the reactor test cell could contain removable-water- shielding tanks to reduce the residual activation dose rates in the test cell after the tests are completed. This reduction will allow the facility to be considered for other uses after the SP-100 tests are completed. The radiation dose rates in the test cell were calculated for several configurations of water-shielding tanks to help evaluate this concept.

Wilcox, A.D.

1991-09-01

14

Clearance of SP-C and recombinant SP-C in vivo and in vitro.  

PubMed

Surfactant protein (SP) C metabolism was evaluated in vivo by measurements of the clearance of bovine native SP-C (nSP-C) and a recombinant SP-C (rSP-C) in rabbits and mice and in vitro by the uptake into MLE-12 cells. rSP-C is the 34-amino acid human sequence with phenylalanine instead of cysteine in positions 4 and 5 and isoleucine instead of methionine in position 32. Alveolar clearances of iodinated SP-C and rSP-C after tracheal instillation were similar and slower than those for dipalmitoyl phosphatidylcholine (DPC) in the rabbit. nSP-C and rSP-C were cleared from rabbit lungs similarly to DPC, each with a half-life (t1/2) of approximately 11 h. In mice, the clearance of rSP-C from the lungs was slower (t1/2 28 h) than the clearance of DPC (t1/2 12 h). Liposome-associated dinitrophenyl-labeled rSP-C was taken up by MLE-12 cells, and the uptake was inhibited by excess nSP-C. The pattern of inhibition of dinitrophenyl-rSP-C uptake by SP-B, but not by SP-A, was similar to that previously reported for nSP-C. Clearance kinetics of nSP-C were similar to previous measurements of pulmonary clearance of SP-B in rabbits and mice. rSP-C has clearance kinetics and uptake by cells similar to those of nSP-C. PMID:9609732

Ikegami, M; Horowitz, A D; Whitsett, J A; Jobe, A H

1998-06-01

15

Selective C(sp2)-C(sp) bond cleavage: the nitrogenation of alkynes to amides.  

PubMed

Breakthrough: A novel catalyzed direct highly selective C(sp2)-C(sp) bond functionalization of alkynes to amides has been developed. Nitrogenation is achieved by the highly selective C(sp2)-C(sp) bond cleavage of aryl-substituted alkynes. The oxidant-free and mild conditions and wide substrate scope make this method very practical. PMID:23804537

Qin, Chong; Feng, Peng; Ou, Yang; Shen, Tao; Wang, Teng; Jiao, Ning

2013-06-26

16

The Sp(1)-Kepler problems  

SciTech Connect

Let n{>=}2 be a positive integer. To each irreducible representation {sigma} of Sp(1), an Sp(1)-Kepler problem in dimension (4n-3) is constructed and analyzed. This system is superintegrable, and when n=2 it is equivalent to a generalized MICZ-Kepler problem in dimension of 5. The dynamical symmetry group of this system is O-tilde*(4n) with the Hilbert space of bound states H({sigma}) being the unitary highest weight representation of O*-tilde(4n) with highest weight, (-1,{center_dot}{center_dot}{center_dot},-1,-(1+{sigma})), which occurs at the rightmost nontrivial reduction point in the Enright-Howe-Wallach classification diagram for the unitary highest weight modules. Here {sigma} is the highest weight of {sigma}. Furthermore, it is shown that the correspondence {sigma}{r_reversible}H({sigma}) is the theta-correspondence for dual pair (Sp(1),O*(4n))subset Sp(8n,R)

Meng Guowu [Department of Mathematics, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon (Hong Kong)

2009-07-15

17

SP-100 control system modeling  

NASA Astrophysics Data System (ADS)

SP-100 Control Systems modeling was done using a thermal hydraulic transient analysis model called ARIES-S. The ARIES-S Computer Simulation provides a basis for design, integration and analysis of the reactor including the control and protection systems. It is a modular digital computer simulation written in FORTRAN that operates interactively in real time on a VAX minicomputer.

Meyer, R. A.; Halfen, F. J.; Alley, A. D.

18

Transcription factor Sp3 antagonizes activation of the ornithine decarboxylase promoter by Sp1.  

PubMed Central

Ornithine decarboxylase (ODC) expression is important for proliferation and is elevated in many tumor cells. We previously showed that Sp1 is a major positive regulator of ODC transcription. In this paper we have investigated transcriptional regulation of rat ODC by the closely related factor Sp3. While over-expression of Sp1 caused a dramatic activation of the ODC promoter, over-expression of Sp3 caused little or no activation in either Drosophila SL2 cells (lacking endogenous Sp1 or Sp3) or in H35 rat hepatoma cells. Furthermore, co-transfection studies demonstrated that Sp3 abolished trans -activation of the ODC promoter by Sp1. DNase I footprint studies and electrophoretic mobility shift assays demonstrated that both recombinant Sp1 and Sp3 bind specifically to several sites within the ODC promoter also protected by nuclear extracts, including overlapping GC and CT motifs located between -116 and -104. This CT element is a site of negative ODC regulation. Mutation of either element reduced binding, but mutation of both sites was required to eliminate binding of either Sp1 or Sp3. These results demonstrate that ODC is positively regulated by Sp1 and negatively regulated by Sp3, suggesting that the ratio of these transcription factors may be an important determinant of ODC expression during development or transformation.

Kumar, A P; Butler, A P

1997-01-01

19

Description of Actinomycetospora chibensis sp. nov., Actinomycetospora chlora sp. nov., Actinomycetospora cinnamomea sp. nov., Actinomycetospora corticicola sp. nov., Actinomycetospora lutea sp. nov., Actinomycetospora straminea sp. nov. and Actinomycetospora succinea sp. nov. and emended description of the genus Actinomycetospora.  

PubMed

Eight actinomycete strains that form bud-like spore chains were isolated from various samples collected in Japan. Phylogenetically, the isolates formed a single clade with the type strain of Actinomycetospora chiangmaiensis according to 16S rRNA gene sequence analysis. The isolates contained meso-diaminopimelic acid, d-glutamic acid and d- and l- alanine in the cell-wall peptidoglycan, arabinose and galactose as characteristic sugars, phosphatidylcholine and phosphatidylethanolamine as diagnostic phospholipids, MK-8(H(4)) as the predominant isoprenoid quinone, iso-C(16 : 0) as the major cellular fatty acid and DNA G+C contents of 72-74 mol%. Actinomycetospora chiangmaiensis, the type species of the genus Actinomycetospora, was also found to contain MK-8(H(4)) predominantly in our study, although it was earlier reported to contain MK-9(H(4)) as the predominant isoprenoid quinone. On the basis of the morphological, physiological, chemotaxonomic, phylogenetic and DNA-DNA hybridization data, we concluded that the isolates can be accommodated in the genus Actinomycetospora with emendation of the description of the genus and are assigned to the following seven novel species: Actinomycetospora chibensis sp. nov. (type strain TT04-21(T) ?= NBRC 103694(T) ?= KACC 14256(T)), Actinomycetospora chlora sp. nov. (type strain TT07I-57(T) ?= NBRC 105900(T) ?= KACC 14252(T)), Actinomycetospora cinnamomea sp. nov. (type strain IY07-53(T) ?= NBRC 105527(T) ?= KACC 14250(T)), Actinomycetospora corticicola sp. nov. (type strain 014-5(T) ?=?NBRC 103689(T) ?= KACC 14253(T)), Actinomycetospora lutea sp. nov. (type strain TT00-04(T) ?= NBRC 103690(T) ?=?KACC 14254(T)), Actinomycetospora straminea sp. nov. (type strain IY07-55(T) ?= NBRC 105528(T) ?= KACC 14251(T)) and Actinomycetospora succinea sp. nov. (type strain TT00-49(T) ?= NBRC 103691(T) ?=?KACC 14255(T)). PMID:20622052

Tamura, Tomohiko; Ishida, Yuumi; Hamada, Moriyuki; Otoguro, Misa; Yamamura, Hideki; Hayakawa, Masayuki; Suzuki, Ken-Ichiro

2010-07-09

20

An ultrastructural study of the spermatozoa of Eulalia sp. (Phyllodocidae), Lepidonotus sp. (Polynoidae), Lumbrineris sp. (Lumbrineridae) and Owenia fusiformis (Oweniidae)  

NASA Astrophysics Data System (ADS)

The ultrastructure of the mature spermatozoa of four polychaetes is described: Eulalia sp. (Phyllodocidae), Lepidonotus sp. (Polynoidae), Lumbrineris sp. (Lumbrineridae) and Owenia fusiformis (Oweniidae). All the sperm show features typical of externally fertilizing sperm in having a rounded nucleus, a short unmodified midpiece, and a simple flagellum with a 9+2 axoneme. Owenia fusiformis and Lepidonotus sp. have a nuclear cone extending into the subacrosomal space that may act to present the inner acrosomal membrane to the egg during fertilization. The acrosome of Lumbrineris sp. is flattened and crenulated. The sperm of Eulalia sp. is unusual in having the four mitochondria of the midpiece ensheathed by a membrane. Comparisons are made with other polychaete sperm, and the use of sperm ultrastructure as a taxonomic tool within the Polychaeta is discussed.

Rouse, G. W.

1988-03-01

21

Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov. Isolated from Clinical Specimens of Human Origin?  

PubMed Central

Three groups of previously unknown gram-positive, anaerobic, coccus-shaped bacteria were characterized using phenotypic and molecular taxonomic methods. Phenotypic and genotypic data demonstrate that these organisms are distinct, and each group represents a previously unknown subline within Clostridium cluster XIII. Two groups are most closely related to Peptoniphilus harei in the genus Peptoniphilus, and the other group is most closely related to Anaerococcus lactolyticus in the genus Anaerococcus. Based on the findings, three novel species, Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov., are proposed. The type strains of Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov. are WAL 10418T (= CCUG 53341T = ATCC BAA-1383T), WAL 12922T (= CCUG 53342T = ATCC BAA-1384T), and WAL 17230T (= CCUG 53340T = ATCC BAA-1385T), respectively.

Song, Yuli; Liu, Chengxu; Finegold, Sydney M.

2007-01-01

22

Pantoea rodasii sp. nov., Pantoea rwandensis sp. nov. and Pantoea wallisii sp. nov., isolated from Eucalyptus.  

PubMed

Several Gram-negative-staining, facultatively anaerobic bacterial isolates were obtained from Eucalyptus seedlings showing symptoms of bacterial blight and dieback in Colombia, Rwanda and South Africa. Partial 16S rRNA gene sequencing, together with partial gyrB sequencing, placed the isolates in the genus Pantoea and indicated that they constituted three novel species. Multilocus sequence analysis (MLSA) based on partial sequences of gyrB, rpoB, infB and atpD revealed Pantoea dispersa, Pantoea eucrina and Pantoea cypripedii as their closest phylogenetic relatives. DNA-DNA hybridization studies confirmed the classification of the new isolates as three novel species and phenotypic tests allowed them to be differentiated from their closest phylogenetic neighbours. The names Pantoea rodasii sp. nov. [type strain LMG 26273(T)=BD 943(T) (deposited with the Plant Pathogenic and Plant Protecting Bacteria Collection, South Africa)=BCC 581(T) (deposited with the Bacterial Culture Collection, Forestry and Agricultural Institute, South Africa)], Pantoea rwandensis sp. nov. (type strain LMG 26275(T)=BD 944(T)=BCC 571(T)) and Pantoea wallisii sp. nov. (type strain LMG 26277(T)=BD 946(T)=BCC 682(T)) are proposed. PMID:21841003

Brady, Carrie L; Cleenwerck, Ilse; van der Westhuizen, Lorinda; Venter, Stephanus N; Coutinho, Teresa A; De Vos, Paul

2011-08-12

23

Transcription factors Sp1 and Sp4 regulate TRPV1 gene expression in rat sensory neurons  

PubMed Central

Background The capsaicin receptor, transient receptor potential vanilloid type -1 (TRPV1) directs complex roles in signal transduction including the detection of noxious stimuli arising from cellular injury and inflammation. Under pathophysiologic conditions, TRPV1 mRNA and receptor protein expression are elevated in dorsal root ganglion (DRG) neurons for weeks to months and is associated with hyperalgesia. Building on our previous isolation of a promoter system for the rat TRPV1 gene, we investigated the proximal TRPV1 P2-promoter by first identifying candidate Sp1-like transcription factors bound in vivo to the P2-promoter using chromatin immunoprecipitation (ChIP) assay. We then performed deletion analysis of GC-box binding sites, and quantified promoter activity under conditions of Sp1 / Sp4 over-expression versus inhibition/knockdown. mRNA encoding Sp1, Sp4 and TRPV1 were quantified by qRT-PCR under conditions of Sp1/Sp4 over-expression or siRNA mediated knockdown in cultured DRG neurons. Results Using ChIP analysis of DRG tissue, we demonstrated that Sp1 and Sp4 are bound to the candidate GC-box site region within the endogenous TRPV1 P2-promoter. Deletion of GC-box "a" or "a + b" within the P2- promoter resulted in a complete loss of transcriptional activity indicating that GC-box "a" was the critical site for promoter activation. Co-transfection of Sp1 increased P2-promoter activity in cultured DRG neurons whereas mithramycin-a, an inhibitor of Sp1-like function, dose dependently blocked NGF and Sp1-dependent promoter activity in PC12 cells. Co-transfection of siRNA directed against Sp1 or Sp4 decreased promoter activity in DRG neurons and NGF treated PC12 cells. Finally, electroporation of Sp1 or Sp4 cDNA into cultures of DRG neurons directed an increase in Sp1/Sp4 mRNA and importantly an increase in TRPV1 mRNA. Conversely, combined si-RNA directed knockdown of Sp1/Sp4 resulted in a decrease in TRPV1 mRNA. Conclusion Based on these studies, we now propose a model of TRPV1 expression that is dependent on Sp1-like transcription factors with Sp4 playing a predominant role in activating TRPV1 RNA transcription in DRG neurons. Given that increases of TRPV1 expression have been implicated in a wide range of pathophysiologic states including persistent painful conditions, blockade of Sp1-like transcription factors represents a novel direction in therapeutic strategies.

2011-01-01

24

Thalassospira xiamenensis sp. nov. and Thalassospira profundimaris sp. nov.  

PubMed

Two bacterial strains, M-5T and WP0211T, were isolated from the surface water of a waste-oil pool in a coastal dock and from a deep-sea sediment sample from the West Pacific Ocean, respectively. Analysis of 16S rRNA gene sequences indicated that both strains belonged to the class Alphaproteobacteria and were closely related to Thalassospira lucentensis (96.1 and 96.2 %, gene sequence similarity, respectively). Based on the results of physiological and biochemical tests, as well as DNA-DNA hybridization experiments, it is suggested that these isolates represent two novel species of the genus Thalassospira. Various traits allow both novel strains to be differentiated from Thalassospira lucentensis, including oxygen requirement, nitrate reduction and denitrification abilities and major fatty acid profiles, as well as their ability to utilize six different carbon sources. Furthermore, the novel strains may be readily distinguished from each other by differences in their motility, flagellation, growth at 4 degrees C and 40 degrees C, their ability to hydrolyse Tween 40 and Tween 80, their utilization of 19 different carbon sources and by quantitative differences in their fatty acid contents. It is proposed that the isolates represent two novel species for which the names Thalassospira xiamenensis sp. nov. (type strain, M-5T=DSM 17429T=CGMCC 1.3998T) and Thalassospira profundimaris sp. nov. (type strain, WP0211T=DSM 17430T=CGMCC 1.3997T) are proposed. PMID:17267971

Liu, Chenli; Wu, Yehui; Li, Li; Ma, Yingfei; Shao, Zongze

2007-02-01

25

Lactobacillus pasteurii sp. nov. and Lactobacillus hominis sp. nov.  

PubMed

Strains 1517(T) and 61D(T) were characterized by phenotypic and molecular taxonomic methods. These Gram-positive lactic acid bacteria were homo-fermentative, facultatively anaerobic short rods. They were phylogenetically related to the genus Lactobacillus according to 16S rRNA gene sequence analysis, with 99 % similarity between strain 1517(T) and the type strain of Lactobacillus gigeriorum, and 98.6, 98.5 and 98.4 % between strain 61D(T) and Lactobacillus gasseri, Lactobacillus taiwanensis and Lactobacillus johnsonii, respectively. Multilocus sequence analysis and metabolic analysis of both strains showed variation between the two strains and their close relatives, with variation in the position of the pheS and rpoA genes. The DNA-DNA relatedness of 43.5 % between strain 1517(T) and L. gigeriorum, and 38.6, 29.9 and 39.7 % between strain 61D(T) and L. johnsonii, L. taiwanensis and L. gasseri, respectively, confirmed their status as novel species. Based on phenotypic and genotypic characteristics, two novel species of Lactobacillus are proposed: Lactobacillus pasteurii sp. nov., with 1517(T) ( = CRBIP 24.76(T) = DSM 23907(T)) as the type strain, and Lactobacillus hominis sp. nov., with 61D(T) (=CRBIP 24.179(T) = DSM 23910(T)) as the type strain. PMID:22328611

Cousin, Sylvie; Motreff, Laurence; Gulat-Okalla, Marie-Laure; Gouyette, Catherine; Spröer, Cathrin; Schumann, Peter; Begaud, Evelyne; Bouchier, Christiane; Clermont, Dominique; Bizet, Chantal

2012-02-10

26

Heterotrophic Nitrification by Arthrobacter sp  

PubMed Central

Arthrobacter sp. isolated from sewage oxidized ammonium to hydroxylamine, a bound hydroxylamine compound, a hydroxamic acid, a substance presumed to be a primary nitro compound, nitrite, and nitrate. The concentration of free hydroxylamine-nitrogen reached 15 ?g/ml. The identification of hydroxylamine was verified by mass spectrometric analysis of its benzophenone oxime derivative. The bound hydroxylamine was tentatively identified as 1-nitrosoethanol on the basis of its mass spectrum, chemical reactions, and infrared and ultraviolet spectra. Hydroxylamine formation by growing cells was relatively independent of pH, but the accumulation of nitrite was strongly favored in alkaline solutions. The formation of hydroxylamine but not nitrite was regulated by the carbon to nitrogen ratio of the medium. The hydroxamic acid was the dominant product of nitrification in iron-deficient media, but hydroxylamine, nitrite, and 1-nitrosoethanol formation was favored in iron-rich solutions. Heterotrophic nitrification by Arthrobacter sp. was not inhibited by several compounds at concentrations which totally inhibited autotrophic nitrification.

Verstraete, W.; Alexander, M.

1972-01-01

27

Keratitis due to Chaetomium sp.  

PubMed Central

Aim. To describe keratitis due to Chaetomium sp. occurring in a 65-year-old woman who presented with a corneal ulcer with hypopyon of the right eye with a history of trauma by vegetable matter. Method. Multiple scrapings were obtained from the ulcer. A lactophenol cotton blue wet mount and a Gram-stained smear of the scrapings were made. Scrapings were also inoculated onto various culture media. Results. Direct microscopy of corneal scrapings revealed moderate numbers of septate fungal hyphae. Greenish-yellow-coloured fungal colonies with aerial mycelium were observed in culture of the corneal scrapes. On the basis of colony characteristics and conidial structure, the fungal isolate was identified as Chaetomium sp. The patient was treated with topical natamycin (5%) hourly and cyclopentolate 1% drops 3 times a day. After 4 weeks of therapy, the hypopyon had disappeared, the epithelial defect had healed, and the stromal infiltration had almost completely resolved; the visual acuity of the eye improved from hand movements to (1/2)/60. Conclusion. Fungi of the genus Chaetomium, which are rare causes of human disease (systemic mycosis, endocarditis, subcutaneous lesions), may also cause ocular lesions.

Kaliamurthy, Jayaraman; Kalavathy, Catti Munuswamy; Nelson Jesudasan, Christadoss Arul; Thomas, Philip A.

2011-01-01

28

SP100 start-up control strategy  

Microsoft Academic Search

A control analysis was performed to evaluate the reference and two alternative reactor start-up control strategies for the SP-100, using a detailed nonlinear model of the reactor. The analysis results show that the reference control strategy for the SP-100 adequately meets the current requirements. The two alternative control strategies provide tighter control than the reference strategy. Use of the measured

Raymond A. Meyer; Sang K. Rhow; Kwok K. Wong; Frank J. Halfen

1991-01-01

29

Catechol degradation by immobilized Rhizobium sp.  

PubMed

Entrapped cells of Rhizobium sp. isolated from Lablab purpureus in calcium alginate degraded catechol (1,2-dihydroxybenzene) compared with the free cells. Agitation enhanced its rapid degradation. The versatility of Rhizobium sp. to degrade phenolic substances can be exploited in biotechnological application. PMID:2042401

Gajendiran, N; Mahadevan, A

1991-01-01

30

Five novel Wickerhamomyces- and Metschnikowia-related yeast species, Wickerhamomyces chaumierensis sp. nov., Candida pseudoflosculorum sp. nov., Candida danieliae sp. nov., Candida robnettiae sp. nov. and Candida eppingiae sp. nov., isolated from plants.  

PubMed

On the basis of nucleotide divergences in the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacers (ITS) domain of the rRNA gene, five novel yeast species, Wickerhamomyces chaumierensis sp. nov. (CBS 8565(T) ?= JCM 17246(T)), Candida pseudoflosculorum sp. nov. (CBS 8584(T) ?= JCM 17242(T)), Candida danieliae sp. nov. (CBS 8533(T) ?= JCM 17247(T)), Candida robnettiae sp. nov. (CBS 8580(T) ?= JCM 17243(T)) and Candida eppingiae sp. nov. (CBS 8586(T) ?= JCM 17241(T)), isolated from plants in Thailand and Guyana, are proposed in this study. PMID:20833877

Groenewald, Marizeth; Robert, Vincent; Smith, Maudy Th

2010-09-10

31

Ligands and receptors of lung surfactant proteins SP-A and SP-D.  

PubMed

Surfactant Protein A (SP-A) and D (SP-D) are calcium-dependent collagen-containing lectins, also called collectins, which play a significant role in surfactant homeostasis and pulmonary immunity. The role of SP-A and SP-D in immune defence is well- established. They are known to bind to a range of microbial pathogens that invade the lungs and target them for phagocytic clearance by resident alveolar macrophages. They are also involved in the clearance of apoptotic and necrotic cells and subsequent resolution of pulmonary inflammation. To date, the molecular mechanisms by which SP-A and SP-D interact with various immune cells are poorly understood. In spite of overall structural similarity, SP-A and SP-D show a number of functional differences in their interaction with surface molecules of microorganisms and host cells. The aim of this review is to provide an overview of the current knowledge of ligands and receptors that are known to interact with SP-A and SP-D. PMID:23747872

Jakel, Anne; Qaseem, Asif S; Kishore, Uday; Sim, Robert B

2013-06-01

32

Blastobotrys americana sp. nov., Blastobotrys illinoisensis sp. nov., Blastobotrys malaysiensis sp. nov., Blastobotrys muscicola sp. nov., Blastobotrys peoriensis sp. nov. and Blastobotrys raffinosifermentans sp. nov., novel anamorphic yeast species.  

PubMed

The genus Blastobotrys, which now includes species previously assigned to the synonymous genera Arxula and Sympodiomyces, represents the anamorph of the ascosporogenous genus Trichomonascus. Six novel species are proposed for assignment to Blastobotrys. They were detected from their unique nucleotide sequences in large-subunit rDNA, ITS1-5.8S-ITS2 rDNA, mitochondrial small-subunit rDNA and the cytochrome oxidase II gene. The proposed novel species are Blastobotrys americana sp. nov. (type strain NRRL Y-6844(T)=CBS 10337(T); substrate unknown; Kansas, USA), Blastobotrys illinoisensis sp. nov. (type strain NRRL YB-1343(T)=CBS 10339(T); from forest debris; Illinois, USA), Blastobotrys malaysiensis sp. nov. (type strain NRRL Y-6417(T)=CBS 10336(T); from soil; Malaysia), Blastobotrys muscicola sp. nov. (type strain NRRL Y-7993(T)=CBS 10338(T); from moss; Louisiana, USA), Blastobotrys peoriensis sp. nov. (type strain NRRL YB-2290(T)=CBS 10340(T); from a fungus; Peoria, IL, USA) and Blastobotrys raffinosifermentans sp. nov. (type strain NRRL Y-27150(T)=CBS 6800(T); substrate unknown). PMID:17473275

Kurtzman, Cletus P

2007-05-01

33

Glutamate receptor activation evokes calpain-mediated degradation of Sp3 and Sp4, the prominent Sp-family transcription factors in neurons  

PubMed Central

Sp-family transcription factors (Sp1, Sp3 and Sp4) contain a zinc-finger domain that binds to DNA sequences rich in G-C/T. As assayed by RT-PCR analysis of mRNA, western blot analysis, immunofluorescence, and antibody-dependent “supershift” of DNA-binding assays, the prominent Sp-family factors in cerebral neurons were identified as Sp3 and Sp4. By contrast, glial cells were found to express Sp1 and Sp3. We previously showed that the pattern of G-C/T binding activity of Sp-family factors is rapidly and specifically altered by the calcium influx accompanying activation of glutamate receptors. Here, we demonstrate that Sp-factor activity is also lost after a cerebral ischemia/reperfusion injury in vivo. Consistent with its calcium-dependent nature, we found that glutamate’s effect on Sp-family factors could be blocked by inhibitors of calpains, neutral cysteine proteases activated by calcium. Purified calpain I cleaved Sp3 and Sp4 into products that retained G-C/T-binding activity, consistent with species observed in glutamate-treated neurons. These data provide details of an impact of glutamate-receptor activation on molecular events connected to gene expression.

Mao, Xianrong; Yang, Shao-Hua; Simpkins, James W.; Barger, Steven W.

2007-01-01

34

Relationship between the Unicellular Red Alga Porphyridium sp. and Its Predator, the Dinoflagellate Gymnodinium sp  

PubMed Central

Contamination of algae cultivated outdoors by various microorganisms, such as bacteria, fungi, algae, and protozoa, can affect growth and product quality, sometimes causing fast collapse of the cultures. The main contaminant of Porphyridium cultures grown outdoors in Israel is a Gymnodinium sp., a dinoflagellate that feeds on the alga. Comparison of the effects of various environmental conditions, i.e., pH, salinity, and temperature, on Gymnodinium and Porphyridium species revealed that the Gymnodinium sp. has sharp optimum curves, whereas the Porphyridium sp. has a wider range of optimum conditions and is also more resistant to extreme environmental variables. The mode of preying on the alga was observed, and the specificity of the Gymnodinium sp. for the Porphyridium sp. was shown. In addition, Gymnodinium extract was shown to contain enzymatic degrading activity specific to the Porphyridium sp. cell wall polysaccharide.

Ucko, Michal; Cohen, Ephraim; Gordin, Hillel; Arad, Shoshana (Malis)

1989-01-01

35

Bioaccumulation of total and methyl mercury in three earthworm species (Drawida sp., Allolobophora sp., and Limnodrilus sp.).  

PubMed

We determined total and methyl mercury contents in soil, three earthworm species and their vomitus to study the species-specific differences of mercury bioconcentration in Huludao City, a heavily polluted region by chlor-alkali and nonferrous metal smelting industry in Liaoning Province, northeast China. Total and methyl mercury contents were 7.20 mg/kg and 6.94 ng/g in soil, 1.43 mg/kg and 43.03 ng/g in Drawida sp., 2.80 mg/kg and 336.52 ng/g in Alolobophora sp., respectively. Total mercury contents were 0.966 mg/kg in Drawida sp. vomitus and 4.979 mg/kg in Alolobophora sp. vomitus, respectively. Total mercury contents in earthworms and their vomitus were significantly species-specific different and were both in decreasing with earthworms body lengths, which might due to the growth dilution. Among the soil, earthworms and their vomitus, total mercury contents were in the order of soil > earthworms > earthworm vomitus. Methyl mercury was about 3.01% of total mercury in Drawida sp., 12.02% of total mercury in Alolobophora sp., respectively. It suggested that mercury was mostly in inorganic forms in earthworms. Bioaccumulation factors of methyl mercury from soil to earthworms were much higher than those of total mercury, which suggested that methyl mercury might be more easily absorbed by and accumulated in earthworms because of its lipid solubility. PMID:19779655

Zhang, Zhong Sheng; Zheng, Dong Mei; Wang, Qi Chao; Lv, Xian Guo

2009-09-25

36

Interplay of Posttranslational Modifications in Sp1 Mediates Sp1 Stability during Cell Cycle Progression  

Microsoft Academic Search

Although Sp1 is known to undergo posttranslational modifications such as phosphorylation, glycosylation, acetylation, sumoylation, and ubiquitination, little is known about the possible interplay between the different forms of Sp1 that may affect its overall levels. It is also unknown whether changes in the levels of Sp1 influence any biological cell processes. Here, we identified RNF4 as the ubiquitin E3 ligase

Yi-Ting Wang; Wen-Bin Yang; Wen-Chang Chang; Jan-Jong Hung

2011-01-01

37

Tetracenoquinocin and 5-iminoaranciamycin from a sponge-derived Streptomyces sp. Sp080513GE-26.  

PubMed

Two new anthracyclines, tetracenoquinocin (1) and 5-iminoaranciamycin (2), together with the known compounds aranciamycin (3) and antibiotic SM 173B were isolated from the culture of Streptomyces sp. Sp080513GE-26 associated with a marine sponge, Haliclona sp. The structures of 1 and 2 were established on the basis of extensive NMR and MS analyses along with (13)C-labeling experiments. The compounds 1-3 were evaluated for cytotoxicity against two cancer cell lines. PMID:20192240

Motohashi, Keiichiro; Takagi, Motoki; Shin-Ya, Kazuo

2010-04-23

38

REVIEWS OF TOPICAL PROBLEMS: Magnetic effects induced by sp impurities and defects in nonmagnetic sp materials  

NASA Astrophysics Data System (ADS)

This paper is a review of the state-of-the-art research concerning the first-principle simulation, synthesis, properties, and application prospects of a new class of magnetic materials obtained by doping nonmagnetic sp compounds (oxides, chalcogenides, and borides of nontransition sp elements) or nanostructures (carbon, BN, AIN, and MgO nanotubes) with nonmagnetic sp impurities or nanostructures involving structural vacancies or topological defects.

Ivanovskii, A. L.

2007-10-01

39

Extraordinary Conservation of Cysteines Among Homologous Chironomus Silk Proteins sp185 and sp220  

Microsoft Academic Search

.   Aquatic larvae of the midge, Chironomus tentans, synthesize a 185-kDa silk protein (sp185) with the cysteine-containing motif Cys-X-Cys-X-Cys (where X is any residue) every\\u000a 20–28 residues. We report here the cloning and full-length sequence of cDNAs encoding homologous silk proteins from Chironomus pallidivittatus (sp185) and Chironomus thummi (sp220). Deduced amino acid sequences reveal proteins of nearly identical mass composed

Steven T. Case; Carol Cox; Walter C. Bell; Rosemary T. Hoffman; Jon Martin; Robert Hamilton

1997-01-01

40

Electron transfer kinetics on composite diamond (sp 3)–graphite (sp 2) electrodes  

Microsoft Academic Search

The concept of non-diamond sp2 impurity states as charge transfer mediators on boron-doped diamond (BDD) surface was suggested as an explanation for the electrochemical behavior of synthetic diamond based electrodes. In order to verify this concept, graphite particles (sp2) were deposited on diamond electrodes (sp3) by mechanical abrasion. The behavior of the so prepared diamond–graphite composite electrodes were compared with

I Duo; A Fujishima; Ch Comninellis

2003-01-01

41

Nucleosides from the marine sponge Haliclona sp.  

PubMed

Three known nucleosides were isolated from the sponge Haliclona sp. The structures were established on the basis of NMR data and comparison with those reported, and chemotaxonomic relationships of the sponge nucleosides were discussed. PMID:19323280

Wang, Bin; Dong, Junde; Zhou, Xuefeng; Lee, Kyung Jin; Huang, Riming; Zhang, Si; Liu, Yonghong

42

Sarcocystic Sp. from Swine in Indonesia.  

National Technical Information Service (NTIS)

During routine histological examination of swine diaphragm muscle tissue obtained from a slaughterhouse in Djakarta, Indonesia, Sarcocystis sp. was found encysted in 2 or 23 diaphragms. In both cases the cysts were firmly embedded in the muscle tissue wit...

1972-01-01

43

Composition of lipophylic extracts from two Tinicates, Styela sp. and Phallusia sp. from the Eastern Mediterranean.  

PubMed

Sterols, volatiles and lipids were isolated and identified from lipophylic extracts from two tunicates, Styela sp. and Phallusia sp., occurring in the Eastern Mediterranean. Seventeen sterols were identified. The sterol composition of the two organisms appeared to be similar except for the concentrations of 5alpha-stanols. Both tunicates were characterized by the presence of sterols with a (22Z)-double bond. In the volatiles significant amounts of chlorinated compounds were found (phenols in Styela sp. and hydrocarbons in Phallusia sp.). The fatty acid composition of triacylglycerols and phospholipids of the two tunicates showed significant differences. PMID:12132698

Slantchev, Krasimir; Yalçin, Funda; Ersöz, Tayfun; Nechev, Jordan; Cali?, Ihsan; Stefanov, Kamen; Popov, Simeon

44

Sp(3) structures on 14-dimensional manifolds  

NASA Astrophysics Data System (ADS)

The present article investigates Sp(3) structures on 14-dimensional Riemannian manifolds, a continuation of the recent study of manifolds modeled on rank two symmetric spaces (here: SU(6)/Sp(3)). We derive topological criteria for the existence of such a structure and construct large families of homogeneous examples. As a by-product, we prove a general uniqueness criterion for characteristic connections of G structures and that the notions of biinvariant, canonical, and characteristic connections coincide on Lie groups with biinvariant metric.

Agricola, Ilka; Friedrich, Thomas; Höll, Jos

2013-07-01

45

SP-100 scaleup to 40 MWe. [UN  

SciTech Connect

Human space exploration and utilization will require megawatts of electric power for propulsion and surface applications. SP-100 nuclear technology being developed under the DOE/NASA/DOD Ground Engineering System contact is directly applicable to these missions. Designs of three scaleup systems delivering 5, 10 and 40 MWe using SP-100 reactor technology in conjunction with both static and dynamic power conversion technologies provide attractive mass characteristics.

Newkirk, D.W.; Salamah, S.A.; Stewart, S.L.; Pluta, P.R. (General Electric Company, P.O. Box 530954, San Jose, California (USA))

1991-01-10

46

SP-100 scaleup to 40 MWe  

NASA Astrophysics Data System (ADS)

Human space exploration and utilization will require megawatts of electric power for propulsion and surface applications. SP-100 nuclear technology being developed under the DOE/NASA/DOD Ground Engineering System contact is directly applicable to these missions. Designs of three scaleup systems delivering 5, 10 and 40 MWe using SP-100 reactor technology in conjunction with both static and dynamic power conversion technologies provide attractive mass characteristics.

Newkirk, Douglas W.; Salamah, Samir A.; Stewart, Samuel L.; Pluta, Philip R.

1991-01-01

47

S&P 100 Index Option Volatility  

Microsoft Academic Search

Using transaction data on the S&P 100 index options, the authors study the effect of valuation simplifications that are commonplace in previous research on the time-series properties of implied market volatility. Using an American-style algorithm that accounts for the discrete nature of the dividends on the S&P 100 index, they find that spurious negative serial correlation in implied volatility changes

Campbell R Harvey; Robert E Whaley

1991-01-01

48

Heptaketides with antiviral activity from three endolichenic fungal strains Nigrospora sp., Alternaria sp. and Phialophora sp.  

PubMed

Two new heptaketides, (+)-(2S,3S,4aS)-altenuene (1a) and (-)-(2S,3S,4aR)-isoaltenuene (2a), together with six known compounds, (-)-(2R,3R,4aR)-altenuene (1b), (+)-(2R,3R,4aS)-isoaltenuene (2b), 5'-methoxy-6-methyl-biphenyl-3,4,3'-triol (3), alternariol (4), alternariol-9-methyl ether (5), and 4-hydroxyalternariol-9-methyl ether (6) were isolated from the EtOAc extract of an endolichenic fungal strain Nigrospora sphaerica (No.83-1-1-2). Compounds 1a and 1b were separated from enantiomers 1 by chiral HPLC, and so were 2a and 2b from enantiomers 2. Interestingly, 1-6 were also obtained from other two endolichenic fungal strains Alternaria alternata (No.58-8-4-1) and Phialophora sp. (No.96-1-8-1). The structures of 1-6 were elucidated by means of MS, HR-MS, NMR, and X-ray diffraction. Furthermore, the absolute configurations of 1a-2b were determined by CD experiments and CD calculation. Of these compounds, 4 and 5 showed antiviral activity against herpes simplex virus (HSV) in vitro, with IC(50) values of 13.5 and 21.3 ?M, and with selective index (SI) values of 26.5 and 17.1, respectively. PMID:22613072

He, Jun-Wei; Chen, Guo-Dong; Gao, Hao; Yang, Fan; Li, Xiao-Xia; Peng, Tao; Guo, Liang-Dong; Yao, Xin-Sheng

2012-05-14

49

Specificity of transcriptional regulation by the zinc finger transcription factors Sp1, Sp3, and Egr-1.  

PubMed

The transcription factors Sp1, Sp3, and Egr-1 bind with their zinc finger DNA-binding domains to GC-rich sequences in the regulatory regions of their target genes. The similarity of the DNA-binding sites of Sp1, Sp3, and Egr-1 has triggered the hypothesis that they compete for the same DNA-binding site. We have investigated the specificity of transcriptional regulation by Sp1, Sp3, and Egr-1 using dominant-negative mutants that block the DNA-binding site of Sp1, Sp3, or Egr-1, respectively. The results show that constitutive transcription of Sp1 regulated reporter genes, containing Sp1 sites derived from the aldolase C and p21WAF1/Cip1 genes, or the long terminal repeat of HIV-1, was impaired by dominant-negative mutants of Sp1 and Sp3, but not by a dominant-negative Egr-1. Transcription mediated by Egr-1 was induced by transfection of expression vectors encoding wild-type or mutated Egr-1 or by stimulation of the extracellular signal-regulated protein kinase pathway via an inducible B-Raf-estrogen receptor fusion protein. In all cases transcription of Egr-1-regulated reporter genes, containing Egr-1 binding sites derived from the Egr-1 or the synapsin I gene was impaired by a dominant-negative Egr-1, but not by dominant-negative Sp1 or Sp3 mutants. These results show that there are genuine Sp1/Sp3 or Egr-1 controlled genes showing no cross-regulation of Sp1/Sp3 and Egr-1 through the same DNA-binding site. This does not exclude the existence of composite Sp1/Sp3/Egr-1 binding sites, where competition for a common DNA-binding site occurs. PMID:15523672

Al-Sarraj, Alia; Day, Regina M; Thiel, Gerald

2005-01-01

50

Reduced expression of SP1 and SP4 transcription factors in peripheral blood mononuclear cells in first-episode psychosis.  

PubMed

Alterations of transcription factor specificity protein 4 (SP4) and 1 (SP1) have been linked to different neuropsychiatric diseases. Reduced SP4 and SP1 protein levels in the prefrontal cortex have been associated with bipolar disorder and schizophrenia, respectively, suggesting that both factors could be involved in the pathogenesis of disorders with psychotic features. The aim of this study was to investigate whether the reduction of SP1, SP4 and SP3 protein and mRNA expression in peripheral blood mononuclear cells in the early stages of psychosis may act as a potential biomarker of these disorders. A cross-sectional study of first-episode psychosis patients (n = 14) compared to gender- and age-matched healthy controls (n = 14) was designed. Patients were recruited through the adult mental health services of Parc Sanitari Sant Joan de Déu. Protein and gene expression levels of SP1, SP4 and SP3 were assessed in peripheral blood mononuclear cells of patients with first-episode psychosis and healthy control subjects. We report that protein levels of SP1 and SP4, but not SP3, are significantly reduced in patients compared to controls. In contrast, we did not observe any differences in expression levels for SP1, SP4 or SP3 genes between patient and control groups. In patients, SP4 protein levels were significantly associated with SP1 protein levels. No association was found, however, between protein and gene expression levels for each factor. Our study shows reduced SP1 and SP4 protein levels in first-episode psychosis in lymphocytes, suggesting that these transcription factors are potential peripheral biomarkers of psychotic spectrum disorders in the early stages. PMID:23941741

Fusté, Montserrat; Pinacho, Raquel; Meléndez-Pérez, Iria; Villalmanzo, Núria; Villalta-Gil, Victoria; Haro, Josep Maria; Ramos, Belén

2013-08-12

51

O glycosylation of an Sp1-derived peptide blocks known Sp1 protein interactions.  

PubMed Central

The O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins is dynamic and abundant in the nucleus and cytosol. Several transcription factors, including Sp1, have been shown to contain this modification; however, the functional role of O-GlcNAc in these proteins has not been determined. In this paper we describe the use of the previously characterized glutamine-rich transactivation domain of Sp1 (B-c) as a model to investigate the role of O-GlcNAc in Sp1's transcriptionally relevant protein-to-protein interactions with the TATA-binding-protein-associated factor (TAF110) and holo-Sp1. When the model Sp1 peptide was overexpressed in primate cells, this 97-amino-acid domain of Sp1 was found to contain a dominant O-GlcNAc residue at high stoichiometry, which allowed the mapping and mutagenesis of this glycosylation site. In vitro interaction studies between this segment of Sp1 and Drosophila TAF110 or holo-Sp1 indicate that the O-GlcNAc modification functions to inhibit the largely hydrophobic interactions between these proteins. In HeLa cells, the mutation at the mapped glycosylation site was permissive for transcriptional activation. We propose the hypothesis that the removal of O-GlcNAc from an interaction domain can be a signal for protein association. O-GlcNAc may thereby prevent untimely and ectopic interactions.

Roos, M D; Su, K; Baker, J R; Kudlow, J E

1997-01-01

52

Infection of Glaucocystis nostochinearum (Glaucophyta) by Lagenidium sp. (Oomycota) and its hyperparasite Pythiella sp. (Oomycota)  

Microsoft Academic Search

The glaucophyte Glaucocystis nostochinearum has to our knowledge been observed to be infected by a parasite for the first time. It was found in samples taken from the northernmost freshwater pond in Germany (on the island of Sylt). The fungal parasite was identified as the oomycete Lagenidium sp. which itself was parasitised by another oomycete, Pythiella sp.

Stefanie F. Kühn; Eberhard Schnepf

2002-01-01

53

Genome Sequence of Sphingomonas sp. Strain PAMC 26605, Isolated from Arctic Lichen (Ochrolechia sp.)  

PubMed Central

The endosymbiotic bacterium Sphingomonas sp. strain PAMC 26605 was isolated from Arctic lichens (Ochrolechia sp.) on the Svalbard Islands. Here we report the draft genome sequence of this strain, which could provide further insights into the symbiotic mechanism of lichens in extreme environments.

Shin, Seung Chul; Ahn, Do Hwan; Lee, Jong Kyu; Kim, Su Jin; Hong, Soon Gyu; Kim, Eun Hye

2012-01-01

54

Regulation of neurotrophin-3 gene transcription by Sp3 and Sp4 in neurons.  

PubMed

Neurotrophin-3 (NT-3), a neurotrophin member, plays crucial roles in neuronal development, function and plasticity. Previous studies have demonstrated that NT-3 gene transcription is driven by alternative promoters A and B, located upstream of exons 1A (EIA) and 1B (EIB), respectively. However, the transcription factors and DNA elements that drive NT-3 gene transcription remain to be identified. Here, we analysed the promoter region of the NT-3 gene and found that an NT-3 transcript containing EIB is predominantly expressed in cortical neurons which preferentially utilize promoter B, and two tandemly repeated GC-boxes, located between -100 and -60 base pairs within promoter B, are required for the transcription. Electrophoretic mobility shift and chromatin immunoprecipitation assays revealed that both specificity protein (Sp)3 and Sp4 were able to bind to the Sp1 binding sequences within the GC boxes. Expression of dominant-negative Sp3 and Sp4 small interfering RNA in cortical neurons reduced the activity of the NT-3 gene promoter. Over-expression of Sp1 family members, especially Sp4, resulted in an increase of the NT-3 gene promoter. These findings indicate that the NT-3 gene is a target gene for Sp4 that is abundantly expressed in the brain. PMID:17059557

Ishimaru, Naoki; Tabuchi, Akiko; Hara, Daichi; Hayashi, Hiroyuki; Sugimoto, Takayuki; Yasuhara, Masahiro; Shiota, Jun; Tsuda, Masaaki

2006-10-24

55

Aromatic hydrocarbons removal by immobilized bacteria (Pseudomonas sp., Staphylococcus sp.) in fluidized bed bioreactor  

Microsoft Academic Search

Pseudomonas sp. and Staphylococcus sp. isolated from hydrocarbons- con- taminated river in Morocco appeared to be able to use benzene, toluene, and naphtha- lene as sole source of carbon and energy. These bacterial strains were immobilized in vermiculite and used for removing hydrocarbons from synthetic water polluted by ben- zene, toluene or naphthalene by using a fluidized bed bioreactor. Immobilized

J. TAOUFIK; Y. ZEROUAL; A. MOUTAOUAKKIL; S. MOUSSAID; F. Z. DZAIRI; M. TALBI; A. HAMMOUMI; K. BELGHMI; K. LEE; M. LOUTFI; M. BLAGHEN

2004-01-01

56

IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3.  

PubMed

IL-10 is an 18-kDa cytokine with a key role in homeostatic control of inflammatory and immune responses. We have investigated how transcription of the IL-10 gene is regulated, so as to be able to understand the circumstances of IL-10 expression in both health and disease. In the mouse, IL-10 gene expression is regulated by a TATA-type promoter with a critical cis-acting element containing GGA repeats located at -89 to -77. Its complementary sequence is similar to the cis-acting elements (TCC repeats) in the promoters of genes encoding epidermal growth factor receptor and CD58. All these elements comprise a common CCTCCT sequence with less conserved C + T-rich sequences. Eliminating this CCTCCT sequence results in a marked reduction in promoter activity, suggesting a necessary role in IL-10 gene expression. Despite its dissimilarity to the G + C-rich Sp1 consensus sequence (GC box), Sp1 and Sp3 transcription factors could be shown to bind to this motif. The requirement for Sp1 and Sp3 in transcription of IL-10 was confirmed using Drosophila SL2 cells, which lack endogenous Sp factors. These results suggest that the transcription of IL-10 is positively regulated by both Sp1 and Sp3. PMID:10861063

Tone, M; Powell, M J; Tone, Y; Thompson, S A; Waldmann, H

2000-07-01

57

Nodulation in black locust by the Gammaproteobacteria Pseudomonas sp. and the Betaproteobacteria Burkholderia sp.  

PubMed

Nodulation abilities of bacteria in the subclasses Gammaproteobacteria and Betaproteobacteria on black locust (Robinia pseudoacacia) were tested. Pseudomonas sp., Burkholderia sp., Klebsiella sp., and Paenibacillus sp. were isolated from surface-sterilized black locust nodules, but their nodulation ability is unknown. The aims of this study were to determine if these bacteria are symbiotic. The species and genera of the strains were determined by RFLP analysis and DNA sequencing of 16S rRNA gene. Inoculation tests and histological studies revealed that Pseudomonas sp. and Burkholderia sp. formed nodules on black locust and also developed differentiated nodule tissue. Furthermore, a phylogenetic analysis of nodA and a BLASTN analysis of the nodC, nifH, and nifHD genes revealed that these symbiotic genes of Pseudomonas sp. and Burkholderia sp. have high similarities with those of rhizobial species, indicating that the strains acquired the symbiotic genes from rhizobial species in the soil. Therefore, in an actual rhizosphere, bacterial diversity of nodulating legumes may be broader than expected in the Alpha-, Beta-, and Gammaproteobacteria subclasses. The results indicate the importance of horizontal gene transfer for establishing symbiotic interactions in the rhizosphere. PMID:20542651

Shiraishi, Ayami; Matsushita, Norihisa; Hougetsu, Taizo

2010-06-09

58

Microfungi on the Pandanaceae: Linocarpon lammiae sp. nov., L. siamensis sp. nov. and L. suthepensis sp. nov. are described with a key to Linocarpon species from the Pandanaceae  

Microsoft Academic Search

Linocarpon species are reported from Pan- danaceae in Australia, Brunei, Hong Kong, Nepal, New Zealand, Philippines, Seychelles, Thailand and Vanuatu. Linocarpon lammiae sp. nov. were collected on decaying leaves of Pandanus tectorius in Hong Kong. Linocarpon siamensis sp. nov. and L. suthepensis sp. nov. were collected from decaying leaves of P. pe- netrans in Thailand. These taxa are described, illus-

S. Thongkantha; S. Lumyong; P. Lumyong; S. R. Whitton; K. D. Hyde

59

Flow cytometry of the side population (SP).  

PubMed

The side population (SP) has become an important hallmark for the definition of the stem-cell compartment, especially for the detection of stem cells and for their physical isolation by fluorescence-activated cell sorting (FACS). SP cells are CD34(-) and were discovered using ultraviolet excitation based on the efflux of Hoechst 33342 (Ho342). Although the method works as originally described, the protocol is difficult for most investigators to perform: first, because the ability to discriminate SP cells is based on the differential retention of Ho342 during a functional assay; second, because of the difficulties in setting the right experimental and acquisition conditions; and third, because analysis of the acquired data requires extensive expertise in flow cytometry to accurately detect the SP events. More recently, a new assay based on the efflux of Vybrant DyeCycle Violet stain (DCV) has been documented to discriminate SP cells. This unit contains many helpful pointers to aid the user in obtaining the best possible results with these assays. PMID:23546779

Petriz, Jordi

2013-01-01

60

Zebrafish Sp1-like protein is structurally and functionally comparable to human Sp1.  

PubMed

The transcription factor Sp1 is a regulator of TATA-less genes. It belongs to the Cys?-His? zinc finger domain-containing family. A zebrafish cDNA encoding a peptide homologous to mammalian Sp1 was cloned and inserted into a pET43.1a vector and expressed in Escherichia coli Rosetta (DE3) cells as a Nus-His-tag fusion protein. After induction with isopropyl thiogalactoside, the protein was purified with a Ni-Sepharose column, and approximately 5-8 mg of pure protein was obtained per liter of culture. The primary sequence and the predicted partial tertiary structure of the potential recombinant zebrafish Sp1 protein are similar to those of human Sp1. The DNA affinity precipitation assay and dual-luciferase promoter activity assay further confirm the nature of the recombinant zebrafish Sp1 protein as a transcription factor. Our results show that zebrafish Sp1-like protein is structurally and functionally comparable to human Sp1. PMID:21040790

Lin, Cha-Jang; Hsiao, Tsun-Hsien; Chung, Yi-Shao; Chang, Wen-Ni; Yeh, Trai-Ming; Chen, Bing-Hung; Fu, Tzu-Fun

2010-10-30

61

The structural nif genes of the cyanobacteria Gloeothece sp. and Calothrix sp. share homology with those of Anabaena sp., but the Gloeothece genes have a different arrangement.  

PubMed

Probes carrying the Anabaena sp. strain PCC 7120 nitrogenase reductase (nifH) and nitrogenase (nifK and nifD) genes were hybridized to Southern blots of DNA from the unicellular, aerobic nitrogen-fixing cyanobacterium Gloeothece sp. strain PCC 6909 and from the filamentous cyanobacterium Calothrix sp. strain PCC 7601. These data suggest that the Gloeothece sp. nif structural proteins must be similar to those of other diazotrophs and that the ability for aerobic nitrogen fixation does not reside in the nif protein complex. We also found that the nif structural genes of Gloeothece sp. are clustered, whereas those of Calothrix sp. are arranged more like those of Anabaena sp. PMID:6305921

Kallas, T; Rebière, M C; Rippka, R; Tandeau de Marsac, N

1983-07-01

62

Asymmetric C(sp(2) )?H Activation.  

PubMed

A "niche" topic in the past decade, the asymmetric C?H bond activation has been attracting growing interest over the last few years. Particularly significant advances have been achieved in the field of direct, stereoselective transformations of C(sp(2) )?H bonds. This Concept article intends to showcase different types of asymmetric C(sp(2) )?H bond activation reactions, emphasising both the nature of the stereo-discriminating step and the variability of valuable scaffolds that could be rapidly constructed by means of such strategies. PMID:24108589

Wencel-Delord, Joanna; Colobert, Françoise

2013-09-17

63

Bartonella jaculi sp. nov., Bartonella callosciuri sp. nov., Bartonella pachyuromydis sp. nov. and Bartonella acomydis sp. nov., isolated from wild Rodentia.  

PubMed

Four novel strains of members of the genus Bartonella, OY2-1(T), BR11-1(T), FN15-2(T) and KS2-1(T), were isolated from the blood of wild-captured greater Egyptian jerboa (Jaculus orientalis), plantain squirrel (Callosciurus notatus), fat-tailed gerbil (Pachyuromys duprasi) and golden spiny mouse (Acomys russatus). All the animals were imported to Japan as pets from Egypt, Thailand and the Netherlands. The phenotypic characterization (growth conditions, incubation periods, biochemical properties and cell morphologies), DNA G+C contents (37.4 mol% for strain OY2-1(T), 35.5 mol% for strain BR11-1(T), 35.7 mol% for strain FN15-2(T) and 37.2 mol% for strain KS2-1(T)), and sequence analyses of the 16S rRNA genes indicated that those strains belong to the genus Bartonella. Sequence comparisons of gltA and rpoB genes suggested that all of the strains should be classified as novel species of the genus Bartonella. In phylogenetic trees based on the concatenated sequences of five loci, including the 16S rRNA, ftsZ, gltA and rpoB genes and the ITS region, and on the concatenated deduced amino acid sequences of three housekeeping genes (ftsZ, gltA and rpoB), all strains formed distinct clades and had unique mammalian hosts that could be discriminated from other known species of the genus Bartonella. These data strongly support the hypothesis that strains OY2-1(T), BR11-1(T), FN15-2(T) and KS2-1(T) should be classified as representing novel species of the genus Bartonella. The names Bartonella jaculi sp. nov., Bartonella callosciuri sp. nov., Bartonella pachyuromydis sp. nov. and Bartonella acomydis sp. nov. are proposed for these novel species. Type strains of Bartonella jaculi sp. nov., Bartonella callosciuri sp. nov., Bartonella pachyuromydis sp. nov. and Bartonella acomydis sp. nov. are OY2-1(T) (?=?JCM 17712(T)?=?KCTC 23655(T)), BR11-1(T) (?=?JCM 17709(T)?=?KCTC 23909(T)), FN15-2(T) (?=?JCM 17714(T)?=?KCTC 23657(T)) and KS2-1(T) (?=?JCM 17706(T)?=?KCTC 23907(T)), respectively. PMID:22941296

Sato, Shingo; Kabeya, Hidenori; Fujinaga, Yuta; Inoue, Kai; Une, Yumi; Yoshikawa, Yasuhiro; Maruyama, Soichi

2012-08-31

64

Human Glycolipid Transfer Protein Gene (GLTP) Expression Is Regulated by Sp1 and Sp3  

PubMed Central

Glycolipid transfer protein (GLTP) accelerates glycolipid intermembrane transfer via a unique lipid transfer/binding fold (GLTP fold) that defines the GLTP superfamily and is the prototype for functional GLTP-like domains in larger proteins, i.e. FAPP2. Human GLTP is encoded by the single-copy GLTP gene on chromosome 12 (12q24.11 locus), but regulation of GLTP gene expression remains completely unexplored. Herein, the ability of glycosphingolipids (and their sphingolipid metabolites) to regulate the transcriptional expression of GLTP via its promoter has been evaluated. Using luciferase and GFP reporters in concert with deletion mutants, the constitutive and basal (225 bp; ?78% G+C) human GLTP promoters have been defined along with adjacent regulatory elements. Despite high G+C content, translational regulation was not evident by the mammalian target of rapamycin pathway. Four GC-boxes were shown to be functional Sp1/Sp3 transcription factor binding sites. Mutation of one GC-box was particularly detrimental to GLTP transcriptional activity. Sp1/Sp3 RNA silencing and mithramycin A treatment significantly inhibited GLTP promoter activity. Among tested sphingolipid analogs of glucosylceramide, sulfatide, ganglioside GM1, ceramide 1-phosphate, sphingosine 1-phosphate, dihydroceramide, sphingosine, only ceramide, a nonglycosylated precursor metabolite unable to bind to GLTP protein, induced GLTP promoter activity and raised transcript levels in vivo. Ceramide treatment partially blocked promoter activity decreases induced by Sp1/Sp3 knockdown. Ceramide treatment also altered the in vivo binding affinity of Sp1 and Sp3 for the GLTP promoter and decreased Sp3 acetylation. This study represents the first characterization of any Gltp gene promoter and links human GLTP expression to sphingolipid homeostasis through ceramide.

Zou, Xianqiong; Gao, Yongguang; Ruvolo, Vivian R.; Gardner, Tawnya L.; Ruvolo, Peter P.; Brown, Rhoderick E.

2011-01-01

65

Cyanobactericidal effect of Rhodococcus sp. isolated from eutrophic lake on Microcystis sp.  

PubMed

A bacterium, which was observed in all cultivations of Microcystis sp., was isolated and designated as Rhodococcus sp. KWR2. The growth of bloom-forming cyanobacteria, including four strains of Microcystis aeruginosa and Anabaena variabilis, was suppressed by up to 75-88% by 2% (v/v) culture broth of KWR2 after 5 days. But KWR2 did not inhibit eukaryotic algae, Chlorella vulgaris and Scenedesmus sp. An extracellular algicidal substance produced by KWR2 showed a cyanobactericidal activity of 94% and was water-soluble with a molecular weight of lower than 8 kDa. PMID:20640876

Lee, Young-Ki; Ahn, Chi-Yong; Kim, Hee-Sik; Oh, Hee-Mock

2010-07-18

66

SP40,40 is a constituent of Alzheimer's amyloid  

Microsoft Academic Search

Cerebrospinal fluid (CSF), serum and seminal plasma contain a small amount of SP-40,40, a modulatory protein of the human complement system. The SP-40,40 in each body fluid was different in molecular size on SDS-PAGE, and glioblastoma cells, hepatoma cells and testicular tumor cells produced SP-40,40, while neuroblastoma cells did not. Therefore, it was estimated that CSF SP-40,40 originated in glia

N.-H. Choi-Miura; Y. Ihara; K. Fukuchi; M. Takeda; Y. Nakano; T. Tobe; M. Tomita

1992-01-01

67

SP Monitoring of Intermittent Flow Through Covered-Karst Sinkholes  

Microsoft Academic Search

A year of continuous SP (self-potential) monitoring was combined with high-resolution 3-D GPR surveys and intermittent water table monitoring over two small covered-karst conduits in Tampa, Florida. SP readings were logged over ~30 electrodes at 2-minute intervals. Positive and negative SP anomalies episodically manifested over conduits, suggesting that conduit flow is dynamic, not static. Three distinct SP flow regimes in

P. B. Bumpus; S. E. Kruse

2010-01-01

68

Access to C(sp3)-C(sp2) and C(sp2)-C(sp2) bond formation via sequential intermolecular carbopalladation of multiple carbon-carbon bonds.  

PubMed

A synthetic strategy of 4-benzyl-substituted 1,3-butadiene derivatives through Pd-catalyzed three-component coupling reaction of benzyl chlorides, alkynes, and monosubstituted alkenes is described. This tandem coupling reaction forms a C(sp(3))-C(sp(2)) bond and a C(sp(2))-C(sp(2)) bond sequentially in a single-step operation. PMID:22647071

Wen, Yanmei; Huang, Liangbin; Jiang, Huanfeng

2012-06-05

69

Biopolymer flocculant produced by an Enterobacter sp  

Microsoft Academic Search

A new biopolymer flocculant was produced by Enterobacter sp. BY-29. Flocculating activity increased in the presence of Al , Fe or Fe . The flocculant had flocculating activity not only in inorganic suspensions of kaolin and active carbon but also in organic suspensions of cellulose and yeast. The flocculant was an acidic polysaccharide consisting of glucose, galactose, xylose and galacturonic

Haruhiko Yokoi; Takashi Yoshida; Shingo Mori; Jun Hirose; Sachio Hayashi; Yoshiyuki Takasaki

1997-01-01

70

Achromobacter animicus sp. nov., Achromobacter mucicolens sp. nov., Achromobacter pulmonis sp. nov. and Achromobacter spiritinus sp. nov., from human clinical samples.  

PubMed

The phenotypic and genotypic characteristics of fourteen human clinical Achromobacter strains representing four genogroups which were delineated by sequence analysis of nusA, eno, rpoB, gltB, lepA, nuoL and nrdA loci, demonstrated that they represent four novel Achromobacter species. The present study also characterized and provided two additional reference strains for Achromobacter ruhlandii and Achromobacter marplatensis, species for which, thus far, only single strains are publicly available, and further validated the use of 2.1% concatenated nusA, eno, rpoB, gltB, lepA, nuoL and nrdA sequence divergence as a threshold value for species delineation in this genus. Finally, although most Achromobacter species can be distinguished by biochemical characteristics, the present study also highlighted considerable phenotypic intraspecies variability and demonstrated that the type strains may be phenotypically poor representatives of the species. We propose to classify the fourteen human clinical strains as Achromobacter mucicolens sp. nov. (with strain LMG 26685(T) [=CCUG 61961(T)] as the type strain), Achromobacter animicus sp. nov. (with strain LMG 26690(T) [=CCUG 61966(T)] as the type strain), Achromobacter spiritinus sp. nov. (with strain LMG 26692(T) [=CCUG 61968(T)] as the type strain), and Achromobacter pulmonis sp. nov. (with strain LMG 26696(T) [=CCUG 61972(T)] as the type strain). PMID:23219252

Vandamme, Peter; Moore, Edward R B; Cnockaert, Margo; De Brandt, Evie; Svensson-Stadler, Liselott; Houf, Kurt; Spilker, Theodore; Lipuma, John J

2012-12-06

71

Classification of Achromobacter genogroups 2, 5, 7 and 14 as Achromobacter insuavis sp. nov., Achromobacter aegrifaciens sp. nov., Achromobacter anxifer sp. nov. and Achromobacter dolens sp. nov., respectively.  

PubMed

The phenotypic and genotypic characteristics of seventeen Achromobacter strains representing MLST genogroups 2, 5, 7 and 14 were examined. Although genogroup 2 and 14 strains shared a DNA-DNA hybridization level of about 70%, the type strains of both genogroups differed in numerous biochemical characteristics and all genogroup 2 and 14 strains could by distinguished by nitrite reduction, denitrification and growth on acetamide. Given the MLST sequence divergence which identified genogroups 2 and 14 as clearly distinct populations, the availability of nrdA sequence analysis as a single locus identification tool for all Achromobacter species and genogroups, and the differential phenotypic characteristics, we propose to formally classify Achromobacter genogroups 2, 5, 7 and 14 as four novel Achromobacter species for which we propose the names Achromobacter insuavis sp. nov. (with strain LMG 26845(T) [=CCUG 62426(T)] as the type strain), Achromobacter aegrifaciens sp. nov. (with strain LMG 26852(T) [=CCUG 62438(T)] as the type strain), Achromobacter anxifer sp. nov. (with strain LMG 26857(T) [=CCUG 62444(T)] as the type strain), and Achromobacter dolens sp. nov. (with strain LMG 26840(T) [=CCUG 62421(T)] as the type strain). PMID:23891345

Vandamme, Peter; Moore, Edward R B; Cnockaert, Margo; Peeters, Charlotte; Svensson-Stadler, Liselott; Houf, Kurt; Spilker, Theodore; Lipuma, John J

2013-07-24

72

Uranium accumulation by Pseudomonas sp. EPS5028  

Microsoft Academic Search

Pseudomonas sp. EPS-5028 was examined for the ability to accumulate uranium from solutions. The uptake of uranium by this microorganism is very rapid and is affected by pH but not by temperature, metabolic inhibitors, culture time and the presence of various cations and anions. The amount of uranium absorbed by the cells increased as the uranium concentration of the solution

Ana M. Marqués; Xavier Roca; M. Dolores Simon-Pujol; M. Carmen Fuste; Francisco Congregado

1991-01-01

73

Nitrogen fixation in Asaia sp. (family Acetobacteraceae).  

PubMed

The genus Asaia (family Acetobacteraceae) was first introduced with a single species-Asaia bogorensis and later six more species were described namely A. siamensis, A. krungthepensis, A. lannaensis, A. platycodi, A. prunellae, and A. astilbes. Acetobacteraceae family has been divided into ten genera but, only three of them include nitrogen fixing species: Gluconacetobacter, Acetobacter, and Swaminathania. This article originated from our study primarily aimed to isolate new endosymbiotic nitrogen fixer among Acetobacteraceae during which we have isolated, for the first time in India, four different strains of Asaia sp. from three different sources: Michalia champaca flower, Anopheles mosquito, and ant Tetraponera rufonigra. All the endosymbiotic strains isolated possess the ability to fix nitrogen. Evidence for both nitrogenase activity and the presence of nifH gene in isolated Asaia sp. is presented. Asaia bogorensis (MTCC 4041(T)) and A. siamensis (MTCC 4042(T)), two of the validated type strains available from the repository, were tested positive for the presence of functional nitrogenase. The nifH gene sequences from these type strains were also confirmed and compared with other nitrogen fixing members of the family Acetobacteraceae. Our result corroborate with the previous reports that Asaia sp. are indeed widely distributed in nature but this is the first time demonstration of their functional nitrogenase activity. This study shows Asaia sp. as fourth genera of nitrogen fixing bacteria in the family Acetobacteraceae. PMID:21681635

Samaddar, Neeloy; Paul, Arundhati; Chakravorty, Somnath; Chakraborty, Writachit; Mukherjee, Joydeep; Chowdhuri, Debarati; Gachhui, Ratan

2011-06-18

74

Detection of Plasmodium sp. in capybara  

Microsoft Academic Search

In the present study, we have microscopically and molecularly surveyed blood samples from 11 captive capybaras (Hydrochaeris hydrochaeris) from the Sanctuary Zoo for Plasmodium sp. infection. One animal presented positive on blood smear by light microscopy. Polymerase chain reaction was carried out accordingly using a nested genus-specific protocol, which uses oligonucleotides from conserved sequences flanking a variable sequence region in

Leonilda Correia dos Santos; Sandra Mara Rotter Curotto; Wanderlei de Moraes; Zalmir Silvino Cubas; Maria de Jesus Costa-Nascimento; Ivan Roque de Barros Filho; Alexander Welker Biondo; Karin Kirchgatter

2009-01-01

75

Major Surface Glycoprotein Genes from Pneumocystis carinii f. sp. ratti  

Microsoft Academic Search

Pneumocystis carinii occurs in a variety of mammals, each of which harbors one or more genetically distinct “special forms” of the microbe. Laboratory rats can be infected by two special forms, P. carinii f. sp. ratti and P. carinii f. sp. carinii. P. carinii f. sp. carinii has a variable antigen, the major surface glycoprotein (MSG), the expression of which

Joshua K Schaffzin; Thomas R Garbe; James R Stringer

1999-01-01

76

In vitro toxin production by Fusarium solani f. sp. piperis  

Microsoft Academic Search

Fusarium solani f. sp. piperis (teleomorph: Nectria haematococca f. sp. piperis), causal agent of root rot and stem blight on black pepper (Piper nigrum), produces secondary metabolites with toxigenic properties, capable of inducing vein discoloration in detached leaves and wilting in transpiring microcuttings. Production of F. solani f. sp. piperis (Fsp) toxic metabolites reached a peak after 25 days of

Maria de Lourdes R. Duarte; Simon A. Archer

2003-01-01

77

A physics/circuit-based switching model for carbon-based resistive memory with sp2/sp3 cluster conversion.  

PubMed

The switching mechanism in carbon-based resistive-switching random access memory is modelled using a percolation approach built on the low-temperature transition between phases sp(3) (diamond-like and high-resistive state) and sp(2) (graphite-like and low-resistive state) for a matrix of carbon clusters in a diamond-like carbon film. The switching process is described using a random circuit breaker network with each breaker controlled by the resistance of clusters sp(2)/sp(3). The key feature of the proposed model is the thermal stress-induced transition from sp(2) to sp(3) phase and the electric field-induced transition from sp(3) to sp(2) phase. Compared with experiments on the switching biasing scheme, a good agreement between simulation and measured data validated the accuracy of the proposed model. PMID:22990172

Qin, Shengjun; Zhang, Jinyu; Fu, Di; Xie, Dan; Wang, Yan; Qian, He; Liu, Litian; Yu, Zhiping

2012-10-21

78

A cascading N=1 Sp(2 N+2 M)×Sp(2 N) gauge theory  

Microsoft Academic Search

We study the N=1 Sp(2N+2M)×Sp(2N) cascading gauge theory on a stack of N physical and M fractional (half) D3-branes at the singularity of an orientifolded conifold. In addition to the D3-branes and an O7-plane, the background contains eight D7-branes, which give rise to matter in the fundamental representation of the gauge group. The moduli space of the gauge theory is

Stephen G. Naculich; Howard J. Schnitzer; Niclas Wyllard

2002-01-01

79

Agfa morandi sp. n. (Rhabditida, Agfidae) a parasite of Limax sp. (Gastropoda, Limacidae).  

PubMed

Agfa morandi sp. n. (Rhabditida, Agfidae), a parasite of Limax sp. (Gastropoda, Limacidae) from Py (Pyrenean mountains, France), is described and illustrated. The present species can be separated from the other two members of the same genus, A. flexilis (Rudolphi, 1819) Morand, 1990 and A. tauricus Korol and Spiridonov, 1991, by size measurements, number and disposition of the male's genital papillae, shape of the spicule and number of eggs in the female. PMID:12122432

Ribas, A; Casanova, J C

2002-04-30

80

Longidorus biformis n. sp. and L. glycines n. sp. (Nematoda: Longidoridae): Two Amphimictic Species from Arkansas.  

PubMed

Two new amphimictic species of Longidorus were found in Arkansas. Longidorus biformis n. sp., found in the rhizosphere of hardwood trees along streams in sandy soil in 14 Arkansas locations, is characterized by its long body (5.42-9.50 mm), wide expanded flattened head end, head width 20.0 to 26.0 microm, odontostyle 96 to 125 microm, guide ring 29 to 38 microm posterior to the anterior end, elongate conoid tail, and c' = 0.9-2.1. Females with 2 to 11 vetromedian supplement-like structures were found in 2 of 14 populations of this new species. Longidorus biformis n. sp. is closest to L. seinhorsti Peneva, Loof &Brown, 1998 and L. closelongatus Stoyanov, 1964. Among North American species it is closest to L. glycines n. sp. A distinguishing feature of L. biformis n. sp. is the presence of supplement-like organs in some females. Longidorus glycines n. sp., found in soybean microplots at the Main Research Station, Fayetteville, Arkansas, is characterized by its long body (6.14-8.31 mm), wide offset flattened head end, head width 20.3 to 23.3 microm, odontostyle 87.3 to 99.5 microm, guide ring 22.3 to 26.4 microm posterior to the anterior end, short conoid tail with rounded terminus, and c' = 0.9-1.4. Longidorus glycines n. sp. is closest to L. lusitanicus Macara, 1985. Among North American species it is close to L. biformis n. sp., L. breviannulatus Norton and Hoffman, 1975, and L. crassus Thorne, 1964. Both new species are believed to have four juvenile stages; the first stage was not found for L. biformis n. sp. PMID:19262782

Ye, Weimin; Robbins, R T

2004-03-01

81

Highly selective insertion of arynes into a C(sp)-O(sp3) ? bond.  

PubMed

Arynes react with ethoxyacetylene to afford 2-ethoxyethynylaryl derivatives through a highly chemo- and regioselective formal insertion of the aryne into the C(sp)-O(sp(3)) bond of the alkyne. Computational studies suggest that the reaction does not proceed through a mechanism initiated by the nucleophilic addition of the oxygen atom to the aryne as previously proposed but by the addition of the triple bond of the alkyne to the aryne. PMID:21291202

Laczkowski, Krzysztof Z; García, Diego; Peña, Diego; Cobas, Agustín; Pérez, Dolores; Guitián, Enrique

2011-02-03

82

Coccidioides posadasii infection alters the expression of pulmonary surfactant proteins (SP)-A and SP-D  

PubMed Central

Background Coccidioidomycosis or Valley Fever is caused by Coccidioides in Southwest US and Central America. Primary pulmonary infection is initiated by inhalation of air-borne arthroconidia. Since, lung is the first organ that encounters arthroconidia, different components of the pulmonary innate immune system may be involved in the regulation of host defense. Pulmonary surfactant proteins (SP)-A and SP-D have been recognized to play an important role in binding and phagocytosis of various microorganisms, but their roles in Coccidioides infection are not known. Methods In this study, we studied the changes in amounts of pulmonary SP-A, SP-D and phospholipid in murine model of Coccidioides posadasii infection, and binding of SP-A and SP-D to Coccidioidal antigens. Mice were challenged intranasally with a lethal dose of C. posadasii (n = 30 arthroconidia) and bronchoalveolar lavage fluid (BALF) samples were collected on day 10, post infection. In another group of animals, mice were immunized with protective formalin killed spherule (FKS) vaccine prior to infection. The concentrations of BALF SP-A, SP-D, total phospholipid were measured using enzyme linked immunosorbent assay and biochemical assays. Results We found that in lavage fluid samples of C. posadasii infected mice, the concentrations of total phospholipid, SP-A and SP-D were 17 % (SEM 3.5, p < 0.001), 38 % (SEM 5.8, p < 0.001) and 4 % (SEM 1.3, p < 0.001) of those in lavage fluid samples of non-infected control mice, respectively. However, the concentrations of SP-A and SP-D remained unchanged in BALF samples of C. posadasii protected mice after immunization with FKS vaccine. Also, we found that both SP-A and SP-D bind to Coccidiodal antigens. Conclusion Our results suggest that the C. posadasii infection perturbs the pulmonary SP-A, SP-D, and phospholipids, potentially enabling the disease progression and promoting fungal dissemination.

Awasthi, Shanjana; Magee, D Mitchell; Coalson, Jacqueline J

2004-01-01

83

Genome sequence of Enterobacter sp. strain SP1, an endophytic nitrogen-fixing bacterium isolated from sugarcane.  

PubMed

Enterobacter sp. strain SP1 is an endophytic nitrogen-fixing bacterium isolated from a sugarcane stem and can promote plant growth. The draft genome sequence of strain SP1 presented here will promote comparative genomic studies to determine the genetic background of interactions between endophytic enterobacteria and plants. PMID:23209221

Zhu, Bo; Chen, Mingyue; Lin, Li; Yang, Litao; Li, Yangrui; An, Qianli

2012-12-01

84

Ogataea kolombanensis sp. nov., Ogataea histrianica sp. nov. and Ogataea deakii sp. nov., three novel yeast species from plant sources.  

PubMed

Nine methanol-assimilating yeast strains isolated from olive oil sediments in Slovenia, extra virgin olive oil from Italy and rotten wood collected in Hungary were found to form three genetically separated groups, distinct from the currently recognized yeast species. Sequence analysis from genes of the small subunit (SSU) rRNA, internal transcribed spacer region/5.8S rRNA, large subunit (LSU) rRNA D1/D2 domains and translational elongation factor-1? (EF-1?) revealed that the three closely related groups represent three different undescribed yeast species. Sequence analysis of the LSU rRNA gene D1/D2 domains placed the novel species in the Ogataea clade. The three novel species are designated as Ogataea kolombanensis sp. nov. (type strain: ZIM 2322(T) = CBS 12778(T) = NRRL Y-63657(T)), Ogataea histrianica sp. nov. (type strain: ZIM 2463(T) = CBS 12779(T) = NRRL Y-63658(T)) and Ogataea deakii sp. nov. (type strain: NCAIM Y.01896(T) = CBS 12735(T) = NRRL Y-63656(T)). PMID:23749284

?adež, Neža; Dlauchy, Dénes; Raspor, Peter; Péter, Gábor

2013-06-07

85

Agromyces italicus sp. nov., Agromyces humatus sp. nov. and Agromyces lapidis sp. nov., isolated from Roman catacombs.  

PubMed

A polyphasic study was carried out to clarify the taxonomic positions of three Gram-positive isolates from the Catacombs of Domitilla, Rome (Italy). 16S rRNA gene sequence comparisons placed these strains within the genus Agromyces. The morphological and chemotaxonomic characteristics of these isolates were consistent with the description of the genus Agromyces. The three isolates could be readily distinguished from one another and from representatives of all Agromyces species with validly published names by a broad range of phenotypic characteristics and DNA-DNA relatedness studies. Therefore, these isolates are proposed to represent three novel species of the genus Agromyces, Agromyces italicus sp. nov. (type strain CD1(T)=HKI 0325(T)=DSM 16388(T)=NCIMB 14011(T)), Agromyces humatus sp. nov. (type strain CD5(T)=HKI 0327(T)=DSM 16389(T)=NCIMB 14012(T)) and Agromyces lapidis sp. nov. (type strain CD55(T)=HKI 0324(T)=DSM 16390(T)=NCIMB 14013(T)). PMID:15774677

Jurado, Valme; Groth, Ingrid; Gonzalez, Juan M; Laiz, Leonila; Schuetze, Barbara; Saiz-Jimenez, Cesareo

2005-03-01

86

SP-100 space subsystems development progress  

NASA Astrophysics Data System (ADS)

The space technology effort related to SP-100 subsystems is described in terms of the areas of primary focus and most significant progress. The SP-100 is briefly compared to the Voyager, and detailed descriptions of the converter subsystem, the heat-transport system, and the heat-rejection subsystem. Progress on the converter subsystem includes a high-voltage insulator composed of a single sapphire crystal, a compliant pad of coated niobium, an SiGe thermoelectric (TE) module, and a TE cell assembly. The test of the Nb1Zr piping related to the heat-transport subsystem is described, and the development is reported for the TEM pump and the gas separator. It is concluded that the critical technical issues related to the technologies have been addressed although further efforts are required. Future testing is described for the three major components of the space subsystems including the converter, pump, and the radiator.

Mondt, Jack F.

1991-09-01

87

SP-100 inert gas act activation  

SciTech Connect

As part of the SP-100 test program at the US Department of Energy Field Office, Richland, there are plans to test the SP-100 space reactor in a vacuum in the test facility shown in Figure 1. The vacuum vessel will be in an inert gas atmosphere in the reactor experiment (RX) cell. The upper assembly (UA)/pump cells will also be inerted. The objective is to determine whether the radioactivity levels in the facility exhaust are within permissible levels. This radioactivity comes from leakage of activation products from the inert gas cells into the facility ventilation exhaust stream. The specific activities were calculated for the activation products from the combinations of inert gases that were considered for this facility, for a range of leakage rates, and for leakage from the UA/pump cells into the RX cell, and results are detailed in this report.

Wilcox, A.D.

1991-09-01

88

Carbon sp chains in graphene nanoholes  

NASA Astrophysics Data System (ADS)

Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment.

Castelli, Ivano E.; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

2012-03-01

89

Microbiology of Naturally Fermented Fish (Sardinella sp.)  

Microsoft Academic Search

Microorganisms involved in the natural fermentation of Sardinella sp. were enumerated and characterized. Total heterotrophic bacterial counts obtained after six days of fermentation were 6.23 × 10 cfu\\/g and 5.65 × 10 cfu\\/g for fish treated with 10 and 15% (w\\/v) sodium chloride, respectively. Counts on MacConkey agar increased steadily as fermentation progressed and reached a peak at the sixth

S. C. Achinewhu; E. N. Amadi; I. S. Barimalaa; J. Eke

2004-01-01

90

Benhamycin, novel alkaloid from terrestrial Streptomyces sp  

Microsoft Academic Search

During our screening for bioactive natural compounds from microorganisms, a novel alkaloid has been isolated from a terrestrial Streptomyces sp. isolate NR12, and named as benhamycin (1). This was along with the known metabolites, uracil, thymine, p-hydroxybenzoic acid, 2?-deoxyuridin, tryptophol, indolyl-3-carboxylic acid, and indolyl-3-carbaldehyde. Chemical structure of the novel compound was determined by detailed analysis of its spectroscopic data (extensive

Mohamed Shaaban; Mohamed S. Abdel-Aziz

2007-01-01

91

Pectin lyase from Aspergillus sp. CHY1043  

Microsoft Academic Search

Aspergillus sp. CH-Y-1043 synthesizes pectin lyase when grown on citrus pectin at 37° C. Production is favoured by increased esterification degree of the pectin used as carbon source. This enzyme displays higher activity at pH values of 8.5–8.8 and temperatures of 40–45° C. The optimal substrate for the enzyme was highly esterified pectin and no enzymatic activity was registered on

Luis Delgado; Blanca A. Trejo; Carlos Huitrón; Guillermo Aguilar

1993-01-01

92

SP100 Heat Source Heat Exchanger Design  

Microsoft Academic Search

A conceptual design for a liquid metal to gas counterflow heat exchanger has been developed for use in the SP-100 Closed Brayton Cycle (CBC) Space Reactor Power System (SRPS). The Heat Source Heat Exchanger (HSHX) is required to transfer 80 kWt from the 1350 K lithium reactor coolant to the He\\/Xe working fluid of the Brayton Rotating Unit (BRU). Trade

T. Ted Fallas; Andrew W. Desepte; Robert J. Hill; Georgi B. Manjarrez; Enrique R. Solorzano; Samir A. Salamah; Raphael Yahalom

1994-01-01

93

A cascading /N=1 /Sp(2N+2M)×Sp(2N) gauge theory  

NASA Astrophysics Data System (ADS)

We study the /N=1 /Sp(2N+2M)×Sp(2N) cascading gauge theory on a stack of /N physical and /M fractional (half) D3-branes at the singularity of an orientifolded conifold. In addition to the D3-branes and an O7-plane, the background contains eight D7-branes, which give rise to matter in the fundamental representation of the gauge group. The moduli space of the gauge theory is analyzed and its structure is related to the brane configurations in the dual type IIB theory and in type IIA/M-theory.

Naculich, Stephen G.; Schnitzer, Howard J.; Wyllard, Niclas

2002-08-01

94

PHARMACOLOGIC DOSES OF ASCORBIC ACID REPRESS SPECIFICITY PROTEIN (Sp) TRANSCRIPTION FACTORS AND Sp-REGULATED GENES IN COLON CANCER CELLS  

PubMed Central

Ascorbic acid (vitamin C) inhibits cancer cell growth and there is a controversy regarding the cancer chemoprotective effects of pharmacologic doses of this compound which exhibits pro-oxidant activity. We hypothesized that the anticancer activity of pharmacologic doses of ascorbic acid (< 5 mM) is due, in part, to reactive oxygen species (ROS)-dependent downregulation of specificity protein (Sp) transcription factors Sp1, Sp3 and Sp4 and Sp-regulated genes. In this study, ascorbic acid (1 – 3 mM) decreased RKO and SW480 colon cancer cell proliferation and induced apoptosis and necrosis and this was accompanied by downregulation of Sp1, Sp3 and Sp4 proteins. In addition, ascorbic acid decreased expression of several Sp-regulated genes that are involved in cancer proliferation [hepatocyte growth factor receptor (c-Met), epidermal growth factor receptor (EGFR) and cyclin D1], survival (survivin and bcl-2), and angiogenesis [vascular endothelial growth factor (VEGF) and its receptors (VEGFR1 and VEGFR2)]. Other pro-oxidants such as hydrogen peroxide exhibited similar activities in colon cancer cells and cotreatment with glutathione inhibited these responses. This study demonstrates for the first time that the anticancer activities of ascorbic acid are due, in part, to ROS-dependent repression of Sp transcription factors.

Pathi, Satya S.; Lei, Ping; Sreevalsan, Sandeep; Chadalapaka, Gayathri; Jutooru, Indira; Safe, Stephen

2012-01-01

95

Serodiagnostic antibody responses to Psoroptes sp. infestations in bighorn sheep.  

PubMed

The antibody responses of bighorn sheep (Ovis canadensis) infected with Psoroptes sp. mites were investigated by enzyme linked immunosorbent assay on western blots of P. cuniculi antigens. Serum from 20 Psoroptes sp.-infested bighorn sheep (O. canadensis mexicana, O. canadensis nelsoni, O. canadensis canadensis) from New Mexico, Nevada, California, and Idaho reacted strongly with mite antigens ranging from 12 to 34 kd. Serum from 35 Psoroptes sp.-free bighorn sheep of unknown tick infestation status and from three Psoroptes sp.-free bighorn sheep infested with Dermacentor hunteri ticks did not react with these antigens. Psoroptes sp.-specific antibody responses were present throughout a 16 mo period in one infected bighorn sheep, but were not detectable 8 mo following successful treatment. These results demonstrate that specific serodiagnosis of Psoroptes sp. infestation is feasible in bighorn sheep and suggest that antibody responses are indicative of current or recent infestation. PMID:2023305

Boyce, W M; Jessup, D A; Clark, R K

1991-01-01

96

Calculating carbon biomass of Phaeocystis sp. from microscopic observations  

Microsoft Academic Search

Conversion factors for calculating carbon biomass ofPhaeocystis sp. colonies and free-living cells were determined from microscopic observations and chemical analysis conducted on cultured and naturalPhaeocystis sp. populations originating from the Southern Bight of the North Sea in 1986 and 1987. They allow calculation, in terms of carbon biomass, of the different forms ofPhaeocystis sp. that succeed each other when the

V. Rousseau; S. Mathot; C. Lancelot

1990-01-01

97

Removal of lead (Pb 2+) by the Cyanobacterium Gloeocapsa sp  

Microsoft Academic Search

Pb2+ removal ability of the viable-freshwater cyanobacterium Gloeocapsa sp. was studied in batch experiments. Gloeocapsa sp. was cultured in the Medium 18 with pH adjusted to 3, 4, 5, 6 and 7. Growth was subsequently determined based on the increase of chlorophyll-a content. Gloeocapsa sp. was able to grow at all pH levels tested, except at pH 3. Removal of

Suneerat Raungsomboon; Amnat Chidthaisong; Boosya Bunnag; Duangrat Inthorn; Narumon W. Harvey

2008-01-01

98

IBM SP high-performance networking with a GRF.  

SciTech Connect

Increasing use of highly distributed applications, demand for faster data exchange, and highly parallel applications can push the limits of conventional external networking for IBM SP sites. In technical computing applications we have observed a growing use of a pipeline of hosts and networks collaborating to collect, process, and visualize large amounts of realtime data. The GRF, a high-performance IP switch from Ascend and IBM, is the first backbone network switch to offer a media card that can directly connect to an SP Switch. This enables switch attached hosts in an SP complex to communicate at near SP Switch speeds with other GRF attached hosts and networks.

Navarro, J.P.

1999-05-27

99

Production of cellulases by Thermomonospora sp  

SciTech Connect

Glucose, cellobiose, Avicel, and Solka Floc were utilized as substrates for growth of Thermomonospora sp. in order to study the induction-repression characteristics of its associated cellulase system. While glucose proved to be an effective repressor of the cellulase enzymes, the other three substrates induced relatively high levels of enzyme activity as measured by the filter paper assay. On a unit cell mass basis the highest values of cellulase activity were obtained when Avicel was utilized as the carbon and energy source. The nature of the cellulosic material and its initial concentration were identified as two very important parameters of the induction process.

Moreira, A.R.; Phillips, J.A.; Humphrey, A.E.

1981-06-01

100

SP-100 design, safety, and testing  

SciTech Connect

The SP-100 Program is developing a nuclear reactor power system that can enhance and/or enable future civilian and military space missions. The program is directed to develop space reactor technology to provide electrical power in the range of tens to hundreds of kilowatts. The major nuclear assembly test is to be conducted at the Hanford Site near Richland, Washington, and is designed to validate the performance of the 2.4-MWt nuclear and heat transport assembly. 10 refs., 5 figs.

Smith, G.L.; Cox, C.M.; Mahaffey, M.K.

1990-07-01

101

Cytotoxic Sesterterpenoids from a Sponge Hippospongia sp.  

PubMed Central

One new pentacyclic sesterterpene, hippospongide A (1), and one new scalarane sesterterpenoid, hippospongide B (2), along with six previously reported known scalarane–type sesterterpenes (3–8), were isolated from a sponge Hippospongia sp. The structures of these compounds were elucidated on the basis of their spectroscopic data and comparison of the NMR data with those of known analogues. These metabolites are the first pentacyclic sesterterpene and scalarane-type sesterterpenes to be reported from this genus. Compounds 3–5 exhibited significant cytotoxicity against DLD-1, HCT-116, T-47D and K562 cancer cell lines.

Chang, Yu-Chia; Tseng, Shang-Wei; Liu, Li-Lian; Chou, Yalan; Ho, Yuan-Shing; Lu, Mei-Chin; Su, Jui-Hsin

2012-01-01

102

Wickerhamiella slavikovae sp. nov. and Wickerhamiella goesii sp. nov., two yeast species isolated from natural substrates.  

PubMed

Two novel yeast species were isolated during three independent studies of yeasts associated with natural substrates in Brazil and Taiwan. Analysis of the sequences of the D1/D2 domains of the large subunit rRNA gene showed that these novel species belong to the Wickerhamiella clade. The first was isolated from freshwater and a leaf of sugar cane (Saccharum officinarum) in Brazil and from leaves of Wedelia biflora in Taiwan. Described here as Wickerhamiella slavikovae sp. nov., it differs by 56 nucleotide substitutions and 19 gaps in the D1/D2 region of the large subunit rRNA gene from Candida sorbophila, the least divergent species. The second species, named Wickerhamiella goesii sp. nov., was isolated from leaves and the rhizosphere of sugar cane collected in Rio de Janeiro, Brazil. The species differs by 54 nucleotide substitutions and nine gaps in the D1/D2 domains from Candida drosophilae, its least divergent relative. The type strains are Wickerhamiella slavikovae sp. nov. IMUFRJ 52096(T) (= CBS 12417(T) = DBVPG 8032(T)) and Wickerhamiella goesii sp. nov. IMUFRJ 52102(T) (= CBS 12419(T) = DBVPG 8034(T)). PMID:23710055

Hagler, Allen N; Ribeiro, José R A; Pinotti, T; Brandão, Luciana R; Pimenta, Raphael S; Lins, U; Lee, Ching-Fu; Hsieh, Chin-Wen; Lachance, Marc-André; Rosa, Carlos A

2013-05-24

103

SP-B and SP-C Alter Diffusion in Bilayers of Pulmonary Surfactant  

PubMed Central

The hydrophobic proteins SP-B and SP-C promote rapid adsorption of pulmonary surfactant to an air/water interface by an unknown mechanism. We tested the hypothesis that these proteins accelerate adsorption by disrupting the structure of the lipid bilayer, either by a generalized increase in fluidity or by a focal induction of interfacial boundaries within the bilayer. We used fluorescence recovery after photobleaching to measure diffusion of nitrobenzoxadiazolyl-dimyristoyl-phosphatidylethanolamine between 11 and 54°C in multilayers containing the complete set of lipids and proteins in calf lung surfactant extract (CLSE), or the complete set of neutral and phospholipids without the proteins. Above 35°C, Arrhenius plots of diffusion were parallel for CLSE and neutral and phospholipids, but shifted to lower values for CLSE, suggesting that the proteins rigidify the lipid bilayer rather than producing the proposed increase in membrane fluidity. The slopes of the Arrhenius plots for CLSE were steeper below 35°C, suggesting that the proteins induce phase separation at that temperature. The mobile fraction fell below 27°C, consistent with a percolation threshold of coexisting gel and liquid-crystal phases. The induction of lateral phase separation in CLSE, however, does not correlate with apparent changes in adsorption kinetics at this temperature. Our results suggest that SP-B and SP-C accelerate adsorption through a mechanism other than the disruption of surfactant bilayers, possibly by stabilizing a high-energy, highly curved adsorption intermediate.

Schram, Vincent; Hall, Stephen B.

2004-01-01

104

Silicate deficiency and lipid synthesis of marine diatoms. [Chaetoceros gracilis; Hantzschia sp. ; Cyclotella sp  

SciTech Connect

Lipid synthesis of three marine diatoms was studied with a /sup 14/CO/sub 2/ incorporation technique in silicate limited batch cultures. Growth rates were independent of the silicate concentration but the cellular yields were proportional to the initial amount of silicate. At the beginning of the stationary growth phase, lipid synthesis rates per unit culture volume increased by 1.7 times for Chaetoceros gracilis, 3.1 times for Hantzschia sp., and 2.8 times for Cyclotella sp., respectively compared to those during the exponential growth phase. Lipid carbon accounted for as much as 57% of the carbon in C. gracilis, 71% in Hantzschia sp., and 65% in Cyclotella sp., respectively. Additional enrichment with silicate during stationary growth phase allowed the cultures to grow further. Lipid synthesis rates were reduced during the subsequent growth phase, and the growth rates themselves were dependent on the level of biomass achieved during the previous stationary phase. However, the cellular yields were similar and probably controlled by light.

Taguchi, S.; Hirata, J.A.; Laws, E.A.

1987-06-01

105

Complete Genome Sequences of Methylophaga sp. Strain JAM1 and Methylophaga sp. Strain JAM7  

PubMed Central

Methylophaga sp. strains JAM1 and JAM7 have been isolated from a denitrification system. Strain JAM1 was the first Methylophaga strain reported to be able to grow under denitrifying conditions. Here, we report the complete genome sequences of the two strains, which allowed prediction of gene clusters involved in denitrification in strain JAM1.

Villeneuve, Celine; Martineau, Christine; Mauffrey, Florian

2012-01-01

106

Tubulideres seminoli gen. et sp. nov. and Zelinkaderes brightae sp. nov. (Kinorhyncha, Cyclorhagida) from Florida  

NASA Astrophysics Data System (ADS)

One new kinorhynch genus and species and one new species from the genus Zelinkaderes are described from sandy sediment off Fort Pierce, Florida. The new genus and species, Tubulideres seminoli gen. et sp. nov. is characterized by the presence of the first trunk segment consisting of a closed ring, the second segment of a bent tergal plate with a midventral articulation and the following nine segments consisting of a tergal and two sternal plates. Cuspidate spines are not present, but flexible tubules are located on several segments, and in particular concentrated on the ventral side of the second segment. Middorsal spines are present on all trunk segments and are alternatingly offset to a position slightly lateral to the middorsal line. Zelinkaderes brightae nov. sp. is characterized by its spine formula in having middorsal spines on trunk segments 4, 6 and 8-11, lateroventral acicular spines on segment 2, lateral accessory cuspidate spines on segments 2 and 8, ventrolateral cuspidate spines on segments 4-6 and 9, lateroventral acicular spines present on segments 8 and 9, and midterminal, lateral terminal and lateral terminal accessory spines on segment 11. The spine formula of Z. brightae nov. sp. places it in a position in between Z. submersus and a clade consisting of Z. klepali and Z. floridensis. The new findings on Z. brightae nov. sp. have led us to propose an emended diagnosis for the genus.

Sørensen, Martin V.; Heiner, Iben; Ziemer, Ole; Neuhaus, Birger

2007-12-01

107

Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor  

PubMed Central

Background Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. Results 16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized. Nucleotide sequences showed 97% and 96% similarity to Methylobacterium sp. AKB-2008-KU9 and Methylobacterium sp. strain 440. The second isolate, likewise, showed 98% similarity to Actinobacterium OR-221. The sequences were deposited in GenBank as Methylobacterium sp. Obi [accession number HQ537130] and Actinobacterium sp. Mayor [accession number JF799090]. Flocculating activity of 95% was obtained in the presence of Ca2+ and heat-stability was exhibited with retention of above 70% activity at 100°C in 30 min. In addition, bioflocculant yield was about 8.203 g/l. A dose of 1 mg/ml of purified bioflocculant was optimal for the clarification of Kaolin suspension (100 ml) following Jar test. FTIR spectrum revealed the presence of carboxyl and hydroxyl functional groups amongst others. Conclusions The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

2013-01-01

108

Pharmacologic Doses of Ascorbic Acid Repress Specificity Protein (Sp) Transcription Factors and Sp-Regulated Genes in Colon Cancer Cells  

Microsoft Academic Search

Ascorbic acid (vitamin C) inhibits cancer cell growth, and there is a controversy regarding the cancer chemoprotective effects of pharmacologic doses of this compound that exhibits prooxidant activity. We hypothesized that the anticancer activity of pharmacologic doses of ascorbic acid (<5 mM) is due, in part, to reactive oxygen species-dependent downregulation of specificity protein (Sp) transcription factors Sp1, Sp3, and

Satya S. Pathi; Ping Lei; Sandeep Sreevalsan; Gayathri Chadalapaka; Indira Jutooru; Stephen Safe

2011-01-01

109

Genetic complexity of the human surfactant-associated proteins SP-A1 and SP-A2.  

PubMed

Pulmonary surfactant protein A (SP-A) plays a key role in innate lung host defense, in surfactant-related functions, and in parturition. In the course of evolution, the genetic complexity of SP-A has increased, particularly in the regulatory regions (i.e. promoter, untranslated regions). Although most species have a single SP-A gene, two genes encode SP-A in humans and primates (SFTPA1 and SFTPA2). This may account for the multiple functions attributed to human SP-A, as well as the regulatory complexity of its expression by a relatively diverse set of protein and non-protein cellular factors. The interplay between enhancer cis-acting DNA sequences and trans-acting proteins that recognize these DNA elements is essential for gene regulation, primarily at the transcription initiation level. Furthermore, regulation at the mRNA level is essential to ensure proper physiological levels of SP-A under different conditions. To date, numerous studies have shown significant complexity of the regulation of SP-A expression at different levels, including transcription, splicing, mRNA decay, and translation. A number of trans-acting factors have also been described to play a role in the control of SP-A expression. The aim of this report is to describe the genetic complexity of the SFTPA1 and SFTPA2 genes, as well as to review regulatory mechanisms that control SP-A expression in humans and other animal species. PMID:23069847

Silveyra, Patricia; Floros, Joanna

2012-10-12

110

[Effects of different years of planting Pennisetum sp. on the plant- and insect diversity in Pennisetum sp. communities].  

PubMed

This paper studied the effects of 1-, 2- and 3 years of planting Pennisetum sp. on the plant- and insect diversity in the Pennisetum sp. communities, taking the barren mountain land without planting Pennisetum sp. as the control (CK). Compared with CK, the plant species richness in Pennisetum sp. communities with different years of planting was lower, but the coverage was higher. The coverage in the Pennisetum sp. community having been planted for 3 years was the highest, up to 91.6%, and 75.8% higher than the CK. The insect species richness in the Pennisetum sp. communities having been planted for 1, 2 and 3 years was 3.6, 5.3 and 5.6 times of the CK, respectively. The plant- and insect diversity indices, including Simpson index, Shannon index, evenness, Brillouin index, and McIntosh index for the Pennisetum sp. communities with different years of planting were significantly higher than the CK, which indicated that the growth of Pennisetum sp. could affect the plant- and insect diversity. With the increasing year of planting, the plant- and insect diversity in Pennisetum sp. communities tended to be stable. PMID:23359949

Lin, Xing-Sheng; Lin, Zhan-Xi; Lin, Dong-Mei; Lin, Hui; Luo, Hai-Ling; Hu, Ying-Ping; Lin, Chun-Mei; Zhu, Chao-Zhi

2012-10-01

111

The ANL/IBM SP scheduling system  

SciTech Connect

Approximately five years ago scientists discovered that modern UNLX workstations connected with ethernet and fiber networks could provide enough computational performance to compete with the supercomputers. As this concept became increasingly popular, the need for distributed queuing and scheduler systems became apparent. Systems such as DQS from Florida State University were developed and worked very well. Today however, supercomputers such as Argonne National Laboratory`s IBM SP system can provide more CPU and networking speed than can be obtained from these networks of workstations. Nevertheless, because modern super computers look like clusters of workstations developers felt that the scheduling systems previously used on clusters of workstations should still apply. After trying to apply some of these scheduling systems to Argonne`s SP environment it became obvious that these two computer environments have very different scheduling needs. Recognizing this need, and realizing that no one has addressed it, we at Argonne developed a new scheduling system. The approach taken in creating this system was unique in that user input and interaction were encouraged throughout the development process. Thus a scheduler was built that actually works the way the users want it to.

Lifka, D.

1995-02-01

112

Arsenite Oxidase from Ralstonia sp. 22  

PubMed Central

We characterized the aro arsenite oxidation system in the novel strain Ralstonia sp. 22, a ?-proteobacterium isolated from soil samples of the Salsigne mine in southern France. The inducible aro system consists of a heterodimeric membrane-associated enzyme reacting with a dedicated soluble cytochrome c554. Our biochemical results suggest that the weak association of the enzyme to the membrane probably arises from a still unknown interaction partner. Analysis of the phylogeny of the aro gene cluster revealed that it results from a lateral gene transfer from a species closely related to Achromobacter sp. SY8. This constitutes the first clear cut case of such a transfer in the Aro phylogeny. The biochemical study of the enzyme demonstrates that it can accommodate in vitro various cytochromes, two of which, c552 and c554, are from the parent species. Cytochrome c552 belongs to the sox and not the aro system. Kinetic studies furthermore established that sulfite and sulfide, substrates of the sox system, are both inhibitors of Aro activity. These results reinforce the idea that sulfur and arsenic metabolism are linked.

Lieutaud, Aurelie; van Lis, Robert; Duval, Simon; Capowiez, Line; Muller, Daniel; Lebrun, Regine; Lignon, Sabrina; Fardeau, Marie-Laure; Lett, Marie-Claire; Nitschke, Wolfgang; Schoepp-Cothenet, Barbara

2010-01-01

113

Microfungi on the Pandanaceae: Linocarpon lammiae sp. nov., L. siamensis sp. nov. and L. suthepensis sp. nov. are described with a key to Linocarpon species from the Pandanaceae.  

PubMed

Linocarpon species are reported from Pandanaceae in Australia, Brunei, Hong Kong, Nepal, New Zealand, Philippines, Seychelles, Thailand and Vanuatu. Linocarpon lammiae sp. nov. were collected on decaying leaves of Pandanus tectorius in Hong Kong. Linocarpon siamensis sp. nov. and L. suthepensis sp. nov. were collected from decaying leaves of P. penetrans in Thailand. These taxa are described, illustrated and compared with Linocarpon species with similar ascospore morphology and dimensions. Included are a synoptic table, which compares the new species to similar known species, and a dichotomous key to species of Linocarpon known from members of the Pandanaceae. PMID:21156623

Thongkantha, S; Lumyong, S; Lumyong, P; Whitton, S R; McKenzie, E H C; Hyde, K D

114

Contracaecum gibsoni n. sp. and C. overstreeti n. sp. (Nematoda: Anisakidae) from the Dalmatian pelican Pelecanus crispus (L.) in Greek waters: genetic and morphological evidence  

Microsoft Academic Search

Two new species of Contracaecum Railliet & Henry, 1912, previously referred to as C. multipapillatum sp. A and C. multipapillatum sp. B by Nascetti et al. (1990) from the Dalmatian pelican Pelecanus crispus (L.) in the Ambracian Gulf off Greece, are described as C. gibsoni n. sp. and C. overstreeti n. sp., respectively. Morphological analysis and the differential diagnosis of

Simonetta Mattiucci; Michela Paoletti; Adolfo Consuegra Solorzano; Giuseppe Nascetti

2010-01-01

115

Ecology and impact of Allorhogas sp. (Hymenoptera: Braconidae) and Apion sp. (Coleoptera: Curculionoidea) on fruits of Miconia calvescens DC (Melastomataceae) in Brazil  

Microsoft Academic Search

Two fruit-feeding insects, a gall wasp, Allorhogas sp. (Hymenoptera: Braconidae), and a beetle, Apion sp. (Coleoptera: Curculionoidea), were evaluated in their native habitat in Brazil as potential biological control agents of Miconia calvescens DC (Melastomataceae). Allorhogas sp. occurred at two out of three field sites with native populations of M. calvescens, and Apion sp. occurred at all three sites. Both

Francisco R. Badenes-Perez; M. Tracy Johnson

2007-01-01

116

Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses.  

PubMed

The goal of this project was to isolate representative Fe(III)-reducing bacteria from kaolin clays that may influence iron mineralogy in kaolin. Two novel dissimilatory Fe(III)-reducing bacteria, strains G12(T) and G13(T), were isolated from sedimentary kaolin strata in Georgia (USA). Cells of strains G12(T) and G13(T) were motile, non-spore-forming regular rods, 1-2 mum long and 0.6 mum in diameter. Cells had one lateral flagellum. Phylogenetic analyses using the 16S rRNA gene sequence of the novel strains demonstrated their affiliation to the genus Geobacter. Strain G12(T) was most closely related to Geobacter pelophilus (94.7 %) and Geobacter chapellei (94.1 %). Strain G13(T) was most closely related to Geobacter grbiciae (95.3 %) and Geobacter metallireducens (95.1 %). Based on phylogenetic analyses and phenotypic differences between the novel isolates and other closely related species of the genus Geobacter, the isolates are proposed as representing two novel species, Geobacter argillaceus sp. nov. (type strain G12(T)=ATCC BAA-1139(T)=JCM 12999(T)) and Geobacter pickeringii sp. nov. (type strain G13(T)=ATCC BAA-1140(T)=DSM 17153(T)=JCM 13000(T)). Another isolate, strain R7(T), was derived from a primary kaolin deposit in Russia. The cells of strain R7(T) were motile, spore-forming, slightly curved rods, 0.6 x 2.0-6.0 microm in size and with up to six peritrichous flagella. Strain R7(T) was capable of reducing Fe(III) only in the presence of a fermentable substrate. 16S rRNA gene sequence analysis demonstrated that this isolate is unique, showing less than 92 % similarity to bacteria of the Sporomusa-Pectinatus-Selenomomas phyletic group, including 'Anaerospora hongkongensis' (90.2 %), Acetonema longum (90.6 %), Dendrosporobacter quercicolus (90.9 %) and Anaerosinus glycerini (91.5 %). On the basis of phylogenetic analysis and physiological tests, strain R7(T) is proposed to represent a novel genus and species, Pelosinus fermentans gen. nov., sp. nov. (type strain R7(T)=DSM 17108(T)=ATCC BAA-1133(T)), in the Sporomusa-Pectinatus-Selenomonas group. PMID:17220454

Shelobolina, Evgenya S; Nevin, Kelly P; Blakeney-Hayward, Jessie D; Johnsen, Claudia V; Plaia, Todd W; Krader, Paul; Woodard, Trevor; Holmes, Dawn E; Vanpraagh, Catherine Gaw; Lovley, Derek R

2007-01-01

117

Effect of fluorides on Milo maize (Sorghum sp. )  

Microsoft Academic Search

The effects of fluorine (F) compounds on the growth and development of Milo maize (Sorghum sp.) in experiments conducted during the period from 1953 to 1961 are summarized. Hydrofluoric acid (HF) or fluosilicic acid (HâSiFâ) was the volatile fluoride used. Snow Princess and Elizabeth the Queen gladiolus (Gladiolus sp.) were used as indicators to compare the known differential response of

A. E. Hitchcock; P. W. Zimmerman; R. R. Coe

2009-01-01

118

Thioglucosidase activity from Sphingobacterium sp. strain OTG1  

Microsoft Academic Search

Screening for novel thioglucoside hydrolase activity resulted in the isolation of Sphingobacterium sp. strain OTG1 from enrichment cultures containing octylthioglucoside (OTG). OTG was hydrolysed into octanethiol and glucose by cell free extracts. Besides thioglucoside hydrolysis, several other glucoside hydrolase activities were detected in the Sphingobacterium sp. strain OTG1 cell free extract. By adding #-glucosidase inhibitors it was possible to discriminate

G. H. Meulenbeld; S. Hartmans

2001-01-01

119

Unusual Bloom of Tetraselmis sp. in the Valparaiso Bay, Chile.  

National Technical Information Service (NTIS)

A bloom of Tetraselmis sp. was observed during the first days of January of 2006 in the Valparaiso Bay (32 57'S; 71 33'W), producing green coloration of sea and exclusion of phytoplankton species. Previous blooms of Tetraselmis sp. have not been observed ...

A. Silva M. Pizarro R. Iturriaga S. Gallegos

2012-01-01

120

Start-up control strategy in the SP100  

Microsoft Academic Search

A control analysis was performed to evaluate the reference and two alternative reactor start-up control strategies for the SP-100, using a detailed nonlinear model of the reactor. The analysis results show that the reference control strategy for the SP-100 adequately meets the current requirements. Two alternative control strategies which are described in this paper provide tighter control than the reference

John E. Savanyo; Sang K. Rhow; Raymond A. Meyer; Brian F. Coleman; Frank J. Halfen

1991-01-01

121

LOCALIZATION OF SP22 ON HUMAN SPERM OF DIFFERING QUALITY  

EPA Science Inventory

LOCALIZATION OF SP22 ON HUMAN SPERM OF DIFFERING QUALITY. AE Lavers*1, GR Klinefelter2, DW Hamilton1, KP Roberts1, 1University of Minnesota, Minneapolis, MN and 2US EPA, Research Triangle Park, NC. SP22 is a sperm membrane protein that has been implicated in sperm function d...

122

Thermogravimetric characteristics and pyrolysis kinetics of Alga Sagarssum sp. biomass.  

PubMed

Alga Sagarssum sp. can be converted to bio-oil, gas, and char through pyrolysis. In this study, the pyrolysis characteristics and kinetics of Sagarssum sp. were investigated using a thermogravimetric analyzer and tubing reactor, respectively. Sagarssum sp. decomposed below 550°C, but the majority of materials decomposed between 200 and 350°C at heating rates of 5-20°C/min. The apparent activation energy increased from 183.53 to 505.57 kJ mol(-1) with increasing pyrolysis conversion. The kinetic parameters of Sagarssum sp. pyrolysis were determined using nonlinear least-squares regression of the experimental data, assuming second-order kinetics. The proposed lumped kinetic model represented the experimental results well and the kinetic rate constants suggested a predominant pyrolysis reaction pathway from Sagarssum sp. to bio-oil, rather than from Sagarssum sp. to gas. The kinetic rate constants indicated that the predominant reaction pathway was A (Sagarssum sp.) to B (bio-oil), rather than A (Sagarssum sp.) to C (gas; C1-C4). PMID:23665684

Kim, Seung-Soo; Ly, Hoang Vu; Kim, Jinsoo; Choi, Jae Hyung; Woo, Hee Chul

2013-04-06

123

Genome Sequence of Pectobacterium sp. Strain SCC3193  

PubMed Central

We report the complete and annotated genome sequence of the plant-pathogenic enterobacterium Pectobacterium sp. strain SCC3193, a model strain isolated from potato in Finland. The Pectobacterium sp. SCC3193 genome consists of a 516,411-bp chromosome, with no plasmids.

Koskinen, J. Patrik; Laine, Pia; Niemi, Outi; Nykyri, Johanna; Harjunpaa, Heidi; Auvinen, Petri; Paulin, Lars; Pirhonen, Minna; Palva, Tapio

2012-01-01

124

Reaction of aroids to Puccinia paullula f. sp. monsterae  

Microsoft Academic Search

Inoculation of various aroids with Puccinia paullula f. sp, monsterae showed that most (59 out of 66) were immune. Monstera deliciosa, M. standleyana cv. Variegata, M. adansonii var. laniata, M. subpinnata, Epipremnum pinnatum (but not E. pinnatum cv. Aureum) and Typhonodorum lindleyanum are confirmed with various degrees of susceptibility, and Stenospennation sp. is newly reported as a moderately resistant host,

D. E. Shaw

1995-01-01

125

Genome Sequence of the Marine Janibacter Sp. Strain HTCC2649 ?  

PubMed Central

Janibacter sp. strain HTCC2649 is a novel marine member of the Actinobacteria, family Intrasporangiaceae, and is closely related to Janibacter melonis CM2104T and Knoellia sinensis HKI 0119T. The organism was isolated from a sample collected at Hydrostation S south of Bermuda by using high-throughput culturing techniques. Here we present the genome sequence of Janibacter sp. strain HTCC2649.

Thrash, J. Cameron; Cho, Jang-Cheon; Bertagnolli, Anthony D.; Ferriera, Steve; Johnson, Justin; Vergin, Kevin L.; Giovannoni, Stephen J.

2011-01-01

126

Biodiesel from indigenous Indonesian marine microalgae, Nanochloropsis sp  

Microsoft Academic Search

An addition the starches of corn were increasing the lipid content in the Nannochloropsis sp. cells and accelerate the growth. The algal cells of Nannochloropsis sp. with 15-30% of lipid content were directly converted into oil for biodiesel purposes by extraction and esterification, trans- esterification processes. The yield was about 15- 33% base on lipid content. The oil obtained at

Dwi Susilaningsih; Apridah Camelia Djohan

2009-01-01

127

Flagellated endosymbiotic bacteria in a marine Frontonia sp. (Oligohymenophorea, Peniculida)  

Microsoft Academic Search

Summary Frontonia sp. was repeatedly found in sea water samples collected from a shore near Leghorn (Ligurian sea). Endosymbiotic rod shaped bacteria 5-6 µm long were found in the cytoplasm of each Frontonia sp. specimen examined at the fluorescent microscope following DAPI staining procedure. These bacteria, as revealed by electron microscopy, are contained in vacuoles (one or two symbionts are

Giovanna Rosati; Letizia Modeo; Giulio Petroni; Susanna Bertolini

128

Lignin Degradation by Fusarium solani f. sp. glycines  

Technology Transfer Automated Retrieval System (TEKTRAN)

Sudden death syndrome (SDS), caused by the soilborne fungal pathogen Fusarium solani f. sp. glycines, is one of the most important diseases of soybean. Lignin degradation may play a role in the infection, colonization, and survival of the fungus in root tissue . Lignin degradation by F. solani f. sp...

129

The filarial endosymbiont Wolbachia sp. is absent from Setaria equina.  

PubMed

Wolbachia sp. was first reported in filarial nematodes over 25 yr ago. Today, much research is focused on the role of these bacteria in filarial worm biology. The filarial symbionts are closely related to arthropod symbionts, which are known to modify host reproduction and biology through various mechanisms. Similarly, it has been suggested that Wolbachia sp. is essential for long-term survival and reproduction of filariae. We report that Wolbachia sp. 16S rDNA was not found in the equine filarial nematode Setaria equina, using either polymerase chain reaction (PCR) or DNA hybridization. In addition, ultrastructural analysis of adult worms did not reveal the presence of Wolbachia sp. in hypodermal cords or reproductive tissues. These data suggest that like Onchocerca flexuosa and Acanthocheilonema vitae, S. equina may not be dependent on Wolbachia sp. for survival. PMID:12537121

Chirgwin, S R; Porthouse, K H; Nowling, J M; Klei, T R

2002-12-01

130

Heat stress protection in Aspen sp1 transgenic Arabidopsis thaliana.  

PubMed

It is known that the stable protein 1 (SP1) detected in aspen plants remains soluble upon boiling and that sp1 expression in transgenic aspen is resistant to salt stress. Presently, we analyzed the effect of expression of SP1 in Arabidopsis thaliana plants and their response to high temperature stress. After 45 degrees C for 16 h, relative to wild type plants, sp1 transgenic plants exhibited stronger growth and were better in several physiological properties including chlorophyll, chlorophyll fluorescence, water content, proline content, and malondialdehyde content. These preliminarily results suggest that the over-expression of SP1 may notably enhance heat-tolerant level of transgenic A. thaliana plants. PMID:18510869

Zhu, Bo; Xiong, Ai-Sheng; Peng, Ri-He; Xu, Jing; Zhou, Jun; Xu, Jin-Tao; Jin, Xiao-Fen; Zhang, Yang; Hou, Xi-Lin; Yao, Quan-Hong

2008-05-31

131

Longidorus ferrisi n. sp. from California Citrus  

PubMed Central

In October 1999, the authors received fixed specimens of a species of Longidorus from Howard Ferris found about the roots of a citrus tree in Oakville, Napa County, CA. After determining it to be new a species, we requested additional specimens. The samples contained roughly equal numbers of males and females. Longidorus ferrisi n. sp. is most similar to L. elongatus, but can be distinguished by a greater c-ratio (111-187 vs 73-141), a lesser c´ (0.7-1.1 vs 1.0-1.3), a more offset head, a more posterior guide ring (35-40 vs 30-33 ?m), the presence of sperm in the uterus in mature females, and the approximate 1:1 ratio of females to males. Other similar species include L. artemisiae, L. crassus, L. glycines, and L. milanis. Longidorus ferrisi n. sp. differs from L. artemisiae by a lesser a-ratio (74-102 vs 109-155), a lesser c´ value (0.7-1.1 vs 1.0-1.6), a more posterior guide ring (35-40 vs 27-34 ?m), a longer odontostyle (91-108 vs 84-98 ?m), a wider lip region (16-19 vs 14-17 ?m), wider mid-body (53-69 vs 41-52 ?m), and longer spicules (57-65 vs 39-49 ?m). The new species differs substantially from L. crassus by its lip shape and the presence of males, and differs from L. glycines by a shorter body (4.33-5.97 vs 6.14-8.31 mm), a lesser c´ value (0.7-1.1 vs 0.9-1.4), a narrower lip region (16-19 vs 20-23 ?m), wider mid-body (53-69 vs 39-57 ?m), longer spicules (53-69 vs 45-53 ?m), and fewer supplements (7-11 vs 11-17). Longidorus ferrisi n. sp. differs from L. milanis by a longer body (4.33-5.97vs 3.00-4.90 mm), a greater c value (111-187 vs 86-130), a wider mid-body (53-69 vs 43-56 ?m), a different head shape, and longer spicules (53-69 vs 41-54 ?m). The nuclear 18S ribosomal DNA sequence of this species revealed that this species is unique with respect to all sequenced Longidorus species.

Ye, Weimin; Pedram, Majid

2009-01-01

132

Elevated expression and potential roles of human Sp5, a member of Sp transcription factor family, in human cancers  

SciTech Connect

In this report, we describe the expression and function of human Sp5, a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Our experiments employing Gal4-Sp5 fusion proteins reveal multiple transcriptional domains, including a N-terminal activity domain, an intrinsic repressive element, and a C-terminal synergistic domain. Elevated expression of Sp5 was noted in several human tumors including hepatocellular carcinoma, gastric cancer, and colon cancer. To study the effects of the Sp5 protein on growth properties of human cancer cells and facilitate the identification of its downstream genes, we combined an inducible gene expression system with microarray analysis to screen for its transcriptional targets. Transfer of Sp5 into MCF-7 cells that expressed no detectable endogenous Sp5 protein elicited significant growth promotion activity. Several of the constitutively deregulated genes have been associated with tumorigenesis (CDC25C, CEACAM6, TMPRSS2, XBP1, MYBL1, ABHD2, and CXCL12) and Wnt/{beta}-Catenin signaling pathways (BAMBI, SIX1, IGFBP5, AES, and p21{sup WAF1}). This information could be utilized for further mechanistic research and for devising optimized therapeutic strategies against human cancers.

Chen Yongxin [Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan); Guo Yingqiu [Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan); Ge Xijin [Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan); Itoh, Hirotaka [Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan); Watanabe, Akira [Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan); Fujiwara, Takeshi [Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan); Kodama, Tatsuhiko [Division of Molecular Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan); Aburatani, Hiroyuki [Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan) and CREST, Japan Science and Technology Agency, 4-1-8 Honchou Kawaguchi, Saitama (Japan)]. E-mail: haburata-tky@umin.ac.jp

2006-02-17

133

Komagataella populi sp. nov. and Komagataella ulmi sp. nov., two new methanol assimilating yeasts from exudates of deciduous trees.  

Technology Transfer Automated Retrieval System (TEKTRAN)

Two new species of the methanol assimilating ascosporic yeast genus Komagataella are described. Komagataella populi sp. nov. (NRRL YB-455, CBS 12362, type strain) was isolated from an exudate on a cottonwood tree (Populus deltoides), Peoria, Illinois, USA, and Komagataella ulmi sp. nov. (NRRL YB-407...

134

Life cycle and secondary production of two mayflies Leptophlebia sp. and Ephemera sp. in Heizhuchong stream, Hubei, China  

NASA Astrophysics Data System (ADS)

From June 2003 to June 2004, an investigation on the life cycle, production and trophic basis of dominant species of macrozoobenthos community in a second-order river of the Hanjiang River basin, Hubei, China was carried out. The results showed that the dominant species Leptophlebia sp. and Ephemera sp. appeared to develop two generations per year, and there was a small overlap in the two generations of both species. The pupation of Leptophlebia sp. mainly occurred in autumn and winter, while pupation of Ephemera sp. mainly took place in summer and winter. The standing stocks of the Leptophlebia sp. population and Ephemera sp. population had two peaks in a year. The cohort production and cohort P/B ratio of the Leptophlebia sp. population estimated by size frequency method were 19.018 1 g/m2.a and 5.7 respectively, while its annual production and P/B ratio were 38.036 2 g/m2.a and 11.4. The cohort production and cohort P/B ratio of the Ephemera sp. population were 38.015 9 g/m2.a and 5.9, while its annual production and P/B ratio were 76.031 8 g/m2.a and 11.8.

Li, Xiaoyu; Yan, Yunjun

2008-02-01

135

Draft Genome Sequence of a Sphingomonas sp., an Endosymbiotic Bacterium Isolated from an Arctic Lichen Umbilicaria sp.  

PubMed Central

Sphingomonas sp. strain PAMC 26617 has been isolated from an Arctic lichen Umbilicaria sp. on the Svalbard Islands. Here we present the draft genome sequence of this strain, which represents a valuable resource for understanding the symbiotic mechanisms between endosymbiotic bacteria and lichens surviving in extreme environments.

Lee, Jungeun; Shin, Seung Chul; Kim, Su Jin; Kim, Bum-Keun; Hong, Soon Gyu; Kim, Eun Hye; Park, Hyun

2012-01-01

136

Genome Sequence of Sphingomonas sp. Strain PAMC 26621, an Arctic-Lichen-Associated Bacterium Isolated from a Cetraria sp.  

PubMed Central

The lichen-associated bacterial strain Sphingomonas sp. PAMC 26621 was isolated from an Arctic lichen Cetraria sp. on Svalbard Islands. Here we report the draft genome sequence of this strain, which could provide novel insights into the molecular principles of lichen-microbe interactions.

Lee, Hyoungseok; Shin, Seung Chul; Lee, Jungeun; Kim, Su Jin; Kim, Bum-Keun; Hong, Soon Gyu; Kim, Eun Hye

2012-01-01

137

CELLULAR ARCHITECTURAL CHANGES IN THE LIVER OF VIPERA RUSSELLI PARASITIZED BY LARVAE OF ANISAKIS SP. AND CONTRACAECUM SP  

Microsoft Academic Search

Pathological changes are described in the liver of Vipera russelli parasitized by larvae of Anisakis sp., and Contracaecum sp. Histological sections of infected liver were prepared by standard procedure for detailed study. These sections were stained with hematoxylin and eosin and mounted permanently in DPX. Photographs were taken by Nikon Optiphot-II. Prominent degenerative changes observed were: thrombosis in dilated portal

N. KHATOON; A. G. RIZWANA; R. SIDRA; F. M. BILQEES

138

Population-based laboratory surveillance for Giardia sp. and Cryptosporidium sp. infections in a large Canadian health region  

Microsoft Academic Search

BACKGROUND: Giardia lamblia (intestinalis) and Cryptosporidium parvum are the two most important intestinal parasites infecting North Americans but there is a paucity of active population-based surveillance data from Canada. This study determined the incidence of and demographic risk factors for developing Giardia sp. and Cryptosporidium sp. infections in a general Canadian population. METHODS: Population-based laboratory surveillance was conducted among all

Kevin B Laupland; Deirdre L Church

2005-01-01

139

Glycosylceramides from marine green microalga Tetraselmis sp.  

PubMed

Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-?3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown. PMID:23089133

Arakaki, Atsushi; Iwama, Daisuke; Liang, Yue; Murakami, Nagisa; Ishikura, Masaharu; Tanaka, Tsuyoshi; Matsunaga, Tadashi

2012-10-19

140

[Cembranoid diterpenes from soft coral Sinularia sp].  

PubMed

A soft coral Sinularia sp., collected from the South China Sea, was selected to investigate the bioactive and chemical constituents. The EtOAc fraction were isolated by repeatedly silica gel and Sephadex LH-20 column chromatography to obtain lobophytolide A (1), 3-dehydroxylpresinularolide B (2), sarcophine (3), 3 beta-acetoxyisolobophytolide (4), Crassocolide D (5), (3E,7E,11E)-6-acetoxy-3,7,11,15(17)-cembratrien-16,14-olide (6). The structures of compounds 1-6 were determined on the basis of spectroscopic data analysis. All compounds were tested against a small panel of human tumor cell lines. And these compounds were obtained for the first time from this coral. PMID:20394288

Lv, Fang; Wang, Xianjie; Dai, Rongji; Deng, Yulin

2010-01-01

141

SP-100 fuel pin performance analysis  

NASA Astrophysics Data System (ADS)

A modified version of the LIFE code was used to evaluate the performance of a wide range of UO2 and UN fuel pin designs for SP-100 reactors. The analyses covered the available irradiation data on refractory alloy-clad fuel pins and fuel pins for thermoelectric, thermionic, and Stirling engine applications. The LIFE code studies showed that vented UO2 fuel pins with W-26 percent Re cladding can operate with cladding temperatures up to the 1800 K for thermionic reactor applications. At cladding temperatures up to 1500 K, W-26 percent Re and ASTAR-811C cladding can be used for vented or nonvented UO2 fuel pins. Nb-1 percent Zr cladding is not recommended for nonvented pin designs at 1500 K. Similarly, for UN fuel, W-26 percent Re and ASTAR-811C cladding can be used up to 1500 K, whereas, T-111 and Nb-1 percent Zr are limited to temperatures below 1400 K.

Boltax, A.; Sundquist, B. E.

142

Predicting fuel performance for SP-100 conditions  

SciTech Connect

This paper reports on methods for analyzing fuel designs proposed for the thermionic and thermoelectric concepts for SP-100 application. The proposed fuel design for the thermionic concept consisted of fully-enriched oxide fuel clad in chemical vapor deposition (CVD) tungsten, which also served as the emitter for the thermionic fuel element (TFE). The fuel density was 95% of theoretical with the linear heat rate flattened radially by removing fuel from the center of the fuel pellet. The fuel inner diameter varied from approx.0.45 in. at the core center to zero at the edge of the core. The as-fabricated gap between fuel and emitter was 10 mils radial. The emitter thickness was 80 mils, and the outer diameter was 1.099 in. The LIFE-4 code was used for evaluation of this concept after extensive review of the code and development of a procedure that corrects certain deficiencies noted in analysis of several tests.

Baars, R.E.

1985-01-01

143

Physicochemical characterization of Acidiphilium sp. biofilms.  

PubMed

The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X-ray photoelectron spectroscopy (XPS), whereas surface-enhanced infrared absorption spectroscopy indicated the presence of redox-active proteins. PMID:23456979

Malki, Moustafa; Casado, Santiago; López, María Francisca; Caillard, Renaud; Palomares, F Javier; Martín Gago, José A; Vaz-Domínguez, Cristina; Cuesta, Angel; Amils, Ricardo; Fernández, Víctor M; Velez, Marisela; De Lacey, Antonio L; Olea, David

2013-03-01

144

Modified Phenazines from an Indonesian Streptomyces sp.†  

PubMed Central

Fractionation of the extract from the Indonesian Streptomyces sp. ICBB8198 as directed by the antibacterial activity delivered the known phenazine antibiotics griseoluteic acid (1a) and griseolutein A (1b), as well as two new phenazine derivatives (2 and 3). In addition, the known compounds spirodionic acid, dihydrosarkomycins, and 6-ethyl-4-hydroxy-3,5-dimethyl-2H-pyran-2-one (4a), along with the new pyrone, 3,6-diethyl-4-hydroxy-5-methyl-2H-pyran-2-one (4b), were isolated. We report here the isolation, structure elucidation, and antibiotic activity of the new metabolites as well as a hypothetical pathway for the formation of the new phenazine derivatives.

Fotso, Serge; Santosa, Dwi Andreas; Saraswati, Rasti; Yang, Jongtae; Mahmud, Taifo; Zabriskie, T. Mark; Proteau, Philip J.

2010-01-01

145

Modified phenazines from an Indonesian Streptomyces sp.  

PubMed

Fractionation of the extract from the Indonesian Streptomyces sp. ICBB8198 as directed by the antibacterial activity delivered the known phenazine antibiotics griseoluteic acid (1a) and griseolutein A (1b), as well as two new phenazine derivatives (2 and 3). In addition, the known compounds spirodionic acid, dihydrosarkomycins, and 6-ethyl-4-hydroxy-3,5-dimethyl-2H-pyran-2-one (4a), along with the new pyrone 3,6-diethyl-4-hydroxy-5-methyl-2H-pyran-2-one (4b), were isolated. We report here the isolation, structure elucidation, and antibiotic activity of the new metabolites as well as a hypothetical pathway for the formation of the new phenazine derivatives. PMID:20092333

Fotso, Serge; Santosa, Dwi Andreas; Saraswati, Rasti; Yang, Jongtae; Mahmud, Taifo; Zabriskie, T Mark; Proteau, Philip J

2010-03-26

146

Biodegradation of Xanthan Gum by Bacillus sp  

PubMed Central

Strains tentatively identified as Bacillus sp. were isolated from sewage sludge and soil and shown to elaborate extracellular enzymes that degrade the extracellular polysaccharide (xanthan gum, polysaccharide B-1459) of Xanthomonas campestris NRRL B-1459. Enzyme production by one strain was greatly enhanced when the strain was incubated in a mixed culture. Products of degradation were identified as d-glucuronic acid, d-mannose, pyruvylated mannose, 6-O-acetyl d-mannose, and a (1?4)-linked glucan. These products correlate with the known structure of the gum. The complexity of the product mixture indicated that the xanthanase was a mixture of carbohydrases. The xanthanase complexes were similar to one another in temperature stability, pH and temperature optima, degree of substrate degradation, and enzymolysis products. Differences in pH stability, salt tolerance, recoverability, and yields of enzyme were observed.

Cadmus, Martin C.; Jackson, Linda K.; Burton, Kermit A.; Plattner, Ronald D.; Slodki, Morey E.

1982-01-01

147

Oxidation of acyclic terpenoids by Corynebacterium sp.  

PubMed Central

Squalene analogs such as lycopersene, geranylfarnesyl, digeranyl, and 2-hydroxy-2,3-dihydrosqualene and terpene alcohol derivatives such as farnesyl benzyl ether, farnesyl pivalate, geranylgeranyl pivalate, geranyl pivalate, and geranyl benzyl ether were oxidized by Corynebacterium sp. strain SY-79, which was isolated from soil by using squalene as a carbon source. Lycopersene and geranylfarnesyl gave no major product. Digeranyl, geranyl benzyl ether, and geranyl pivalate gave terminal oxidation products, and 2-hydroxy-2,3-dihydrosqualene, farnesyl benzyl ether, farnesyl pivalate, and geranylgeranyl pivalate were degraded to give lower molecular carboxylic acids. Strain SY-79 showed promising oxidative activities toward acyclic terpenes, although the metabolites obtained were variable, depending upon the structure of the substrate.

Yamada, Y; Seo, C W; Okada, H

1985-01-01

148

Sp1/Sp3 and PU.1 differentially regulate beta(5) integrin gene expression in macrophages and osteoblasts.  

PubMed

Murine osteoclast precursors and osteoblasts express the integrin alpha(v)beta(5), the appearance of which on the cell surface is controlled by the beta(5), and not the alpha(v), subunit. Here, we show that a 173-base pair proximal region of the beta(5) promoter mediates beta(5) basal transcription in macrophage (osteoclast precursor)-like and osteoblast-like cells. DNase I footprinting reveal four regions (FP1-FP4) within the 173-base pair region, protected by macrophage nuclear extracts. In contrast, osteoblast nuclear extracts protect only FP1, FP2, and FP3. FP1, FP2, and FP3 bind Sp1 and Sp3 from both macrophage and osteoblast nuclear extracts. FP4 does not bind osteoblast proteins but binds PU.1 from macrophages. Transfection studies show that FP1 and FP2 Sp1/Sp3 sites act as enhancers in both MC3T3-E1 (osteoblast-like) and J774 (macrophage-like) cell lines, whereas the FP3 Sp1/Sp3 site serves as a silencer. Mutation of the FP2 Sp1/Sp3 site totally abolishes promoter activity in J774 cells, with only partial reduction in MC3T3-E1 cells. Finally, we demonstrate that PU.1 acts as a beta(5) silencer in J774 cells but plays no role in MC3T3-E1 cells. Thus, three Sp1/Sp3 sites regulate beta(5) gene expression in macrophages and osteoblast-like cells, with each element exhibiting cell-type and/or activation-suppression specificity. PMID:10722663

Feng, X; Teitelbaum, S L; Quiroz, M E; Cheng, S L; Lai, C F; Avioli, L V; Ross, F P

2000-03-24

149

Longidorus kheirii n. sp. (Nematoda: Longidoridae) from Iran.  

PubMed

Longidorus kheirii n. sp., a parthenogenetic species, was found in soil samples collected from the rhizosphere of Rosa sp. growing in a natural mountainous region close to Maragheh city, northwestern Iran. It is characterised by having a long body (6.7-9 mm), a 19.5-23 mum wide head continuous with the body contour, a truncate and slightly concave lip region with convex sides between the anterior end and the guide-ring, an odontostyle 113-130 mum long, an odontophore 69-97.5 mum long, a body width of 90.5-117.5 mum at the mid-body, a long, wide oesophageal bulb (149.5-193.5 x 39.5-48 mum), a tail length of 47-72 mum, a male with 11 ventromedian supplements and spicules of 85 mum in length, and four juvenile stages. The ribosomal 18S rDNA gene of L. kheirii n. sp., L. leptocephalus Hooper, 1961, L. profundorum Hooper, 1966 L. euonymus Mali & Hooper, 1973 and two unidentified species listed as Longidorus sp. 1 and Longidorus sp. 2, all recovered from northwestern Iran in the same survey, and the ITS1 of L. kheirii n. sp. and Longidorus sp. 1 were sequenced in order to investigate the phylogenetic relationships with other previously sequenced Longidorus species. PMID:18815899

Pedram, Majid; Niknam, Gholamreza; Robbins, Robert T; Ye, Weimin; Karegar, Akbar

2008-09-26

150

Halobacterium sp. GRB: a species to work with!?  

PubMed

The properties of the halobacterial isolate Halobacterium sp. GRB are discussed, especially in relation to its use as a laboratory strain. Experimental results on this species are described, including the isolation of point mutants in the bacterioopsin gene leading to single amino acid replacements in bacteriorhodopsin, the application of a selection procedure for the isolation of different types of mutants, the genetic stability of Halobacterium sp. GRB and the possibility of isolating a set of isogenic mutants, the conditions for transformation experiments with this species, and specific features of Halobacterium sp. GRB, such as halocin production and the absence of a restriction system, as well as DNA adenosine methylation. PMID:2720494

Soppa, J; Oesterhelt, D

1989-01-01

151

Down-regulation of human topoisomerase II? expression correlates with relative amounts of specificity factors Sp1 and Sp3 bound at proximal and distal promoter regions  

PubMed Central

Background Topoisomerase II? has been shown to be down-regulated in doxorubicin-resistant cell lines. The specificity proteins Sp1 and Sp3 have been implicated in regulation of topoisomerase II? transcription, although the mechanism by which they regulate expression is not fully understood. Sp1 has been shown to bind specifically to both proximal and distal GC elements of the human topoisomerase II? promoter in vitro, while Sp3 binds only to the distal GC element unless additional flanking sequences are included. While Sp1 is thought to be an activator of human topoisomerase II?, the functional significance of Sp3 binding is not known. Therefore, we sought to determine the functional relationship between Sp1 and Sp3 binding to the topoisomerase II? promoter in vivo. We investigated endogenous levels of Sp1, Sp3 and topoisomerase II? as well as binding of both Sp1 and Sp3 to the GC boxes of the topoisomerase II? promoter in breast cancer cell lines in vivo after short term doxorubicin exposure. Results Functional effects of Sp1 and Sp3 were studied using transient cotransfection assays using a topoisomerase II? promoter reporter construct. The in vivo interactions of Sp1 and Sp3 with the GC elements of the topoisomerase II? promoter were studied in doxorubicin-treated breast cancer cell lines using chromatin immunoprecipitation assays. Relative amounts of endogenous proteins were measured using immunoblotting. In vivo DNA looping mediated by proteins bound at the GC1 and GC2 elements was studied using the chromatin conformation capture assay. Both Sp1 and Sp3 bound to the GC1 and GC2 regions. Sp1 and Sp3 were transcriptional activators and repressors respectively, with Sp3 repression being dominant over Sp1-mediated activation. The GC1 and GC2 elements are linked in vivo to form a loop, thus bringing distal regulatory elements and their cognate transcription factors into close proximity with the transcription start site. Conclusion These observations provide a mechanistic explanation for the modulation of topoisomerase II? and concomitant down-regulation that can be mediated by topoisomerase II poisons. Competition between Sp1 and Sp3 for the same cognate DNA would result in activation or repression depending on absolute amounts of each transcription factor in cells treated with doxorubicin.

Williams, Amram O; Isaacs, Richard J; Stowell, Kathryn M

2007-01-01

152

Removal of Pb (II) by immobilized and free filaments of marine Oscillatoria sp. NTMS01 and Phormidium sp. NTMS02.  

PubMed

Pb(2+) removal ability of the immobilized and free filaments of marine cyanobacteria Oscillatoria sp. NTMS01 and Phormidium sp. NTMS02 was studied using batch experiments. Biosorption of lead by immobilized filaments was studied as a function of pH (2, 4, 6, 8, 10), contact time (5-180 min) and initial lead concentration (1, 3, 5, 7 mg/L) and the removal efficiency of free filaments was studied by culturing in the marine medium with the initial concentration (1, 3, 5, 7 mg/L) at pH 7 and incubated for 10 days. The maximum percentage removal was observed at 25 min for immobilized Oscillatoria sp. NTMS01 and 30 min for immobilized Phormidium sp. NTMS02. At 4th and 6th day of incubation, 89% and 77% removal was observed at 1 mg/L of initial lead concentration by free filaments of Oscillatoria sp. NTMS01 and Phormidium sp. NTMS02 respectively and further the removal was decreased with increasing concentration. Chlorophyll-a content was decreased in a dose dependent manner. About 40 and 50% reduction of chlorophyll-a was observed at higher concentration in Oscillatoria sp. NTMS01 and Phormidium sp. NTMS02 respectively. The adsorption capacity of immobilized Oscillatoria sp. NTMS01 was found to be (Q(max)) 217.39 which is comparatively higher than other sorbents. The Pb(2+) removal efficiency was performed as described in terms of Langmuir and Freundlich isotherms. These organisms is found to fit better by the Langmuir isotherms. PMID:21720797

Kumar, Muthukannan Satheesh; Rajeshwari, Kamaraj; Johnson, Shani; Thajuddin, Nooruddin; Gunasekaran, Muthukumaran

2011-07-01

153

Plant growth promoting properties of Halobacillus sp. and Halomonas sp. in presence of salinity and heavy metals.  

PubMed

Salinity and heavy metal stress are challenging problems in agriculture. Here we report the plant growth promoting ability of three moderate halophiles, Halobacillus sp. ADN1, Halomonas sp. MAN5, and Halobacillus sp. MAN6, in presence of both salinity and heavy metal stress. Halobacillus sp. ADN1, Halomonas sp. MAN5, and Halobacillus sp. MAN6 can tolerate 25, 21, and 29% NaCl, respectively and grow in presence of 1?mM cobalt, cadmium, and nickel and 0.04?mM mercury and 0.03?mM silver. Halobacillus sp. ADN1, Halomonas sp. MAN5, and Halobacillus sp. MAN6 produced 152.5, 95.3, and 167.3?µg/ml indole acetic acid (IAA) and could solubilize 61, 53, and 75 parts per million (ppm) phosphate, respectively in the presence of 15% NaCl. The production of IAA and solubilization of phosphate was well retained in the presence of salinity and heavy metals like 1?mM cadmium, 0.7?mM nickel, 0.04?mM mercury, and 0.03?mM silver. Besides, the strains showed amylase and protease activities and could produce hydrogen cyanide and ammonia in presence of salinity and heavy metals. A mixture of three strains enhanced the root growth of Sesuvium portulacastrum under saline and heavy metal stress, where the root length increased nearly 4.5?±?0.6 times and root dry weight increased 5.4?±?0.5 times as compared to control. These strains can thus be useful in microbial assisted phytoremediation of polluted saline soils. PMID:23775888

Desale, Prithviraj; Patel, Bhargav; Singh, Sukrit; Malhotra, Aakshi; Nawani, Neelu

2013-06-17

154

Haloferula rosea gen. nov., sp. nov., Haloferula harenae sp. nov., Haloferula phyci sp. nov., Haloferula helveola sp. nov. and Haloferula sargassicola sp. nov., five marine representatives of the family Verrucomicrobiaceae within the phylum 'Verrucomicrobia'.  

PubMed

Six Gram-negative, non-motile, rod-shaped, chemoheterotrophic bacteria designated strains YM23-227(T), 06SJR1-1(T), AK18-024(T), 05IJR53-1(T), MN1-1037(T) and MN1-1047 were isolated from various marine environments and subjected to a polyphasic taxonomic study. Preliminary analysis based on 16S rRNA gene sequences revealed that the novel isolates could be affiliated with the family Verrucomicrobiaceae of the phylum 'Verrucomicrobia'. The strains shared approximately 94-100 % sequence similarity with each other and showed less than 94 % similarity with members of the family Verrucomicrobiaceae with validly published names. The DNA-DNA relatedness between strains YM23-227(T) and 06SJR1-1(T) was less than 70 %, a value that is accepted as a phylogenetic definition of a species. The cell wall peptidoglycan of the strains contained muramic acid and meso-diaminopimelic acid. The novel isolates produced carotenoid pigments and squalene. The DNA G+C contents of the six strains were 63-65 mol%. The major menaquinone was MK-9 and iso-C(14 : 0) was the major fatty acid. Based on the evidence from the polyphasic taxonomic study, it was concluded that the six strains should be classified as representing a new genus and five novel species of the family Verrucomicrobiaceae within the phylum 'Verrucomicrobia', for which the names Haloferula rosea gen. nov., sp. nov. (type strain 06SJR1-1(T)=MBIC08340(T)=KCTC 22201(T)), Haloferula harenae sp. nov. (type strain YM23-227(T)=MBIC08299(T)=KCTC 22198(T)), Haloferula phyci sp. nov. (type strain AK18-024(T)=MBIC08341(T)=KCTC 22200(T)), Haloferula helveola sp. nov. (type strain 05IJR53-1(T)=MBIC08342(T)=KCTC 22199(T)) and Haloferula sargassicola sp. nov. (type strain MN1-1037(T)=MBIC08343(T)=KCTC 22202(T)) are proposed. PMID:18984682

Yoon, Jaewoo; Matsuo, Yoshihide; Katsuta, Atsuko; Jang, Jae-Hyuk; Matsuda, Satoru; Adachi, Kyoko; Kasai, Hiroaki; Yokota, Akira

2008-11-01

155

Flowering and branching in Lathyrus odoratus L.: loci sp and b  

Microsoft Academic Search

A second flowering gene, Sp, which influences sensitivity to photoperiod, is identified in the sweet pea, Lathyrus odoratus L. Genes Sp and Dnh act in a complementary manner to confer the summer-flowering phenotype and a near obligate long day requirement for flowering in the unvernalized state. Mutations sp and Dni each diminish the response to photoperiod, and genotypes sp Dnh

J. J. Ross; I. C. Murfet

1988-01-01

156

Occurrence of Diplostomum sp. (Trematoda: Diplostomatidae) in Pond-Reared Walleyes from Michigan  

Microsoft Academic Search

Four hundred ninety walleye fingerlings (Stizostedion vitreum) collected in June and November 1986 and in May and June 1987 from seven rearing ponds in Michigan's Lower Peninsula were examined for parasites. Diplostomum sp., Spiroxys sp., and Camallanus sp. were the only parasites found. Of these helminths, Diplostomum sp. occurred most frequently, infecting walleyes from three ponds. Macroscopic pathological conditions associated

Patrick M. Muzzall; Robert D. Sweet; Craig L. Milewski

1990-01-01

157

Ibm sp high-performance networking with a grf.  

National Technical Information Service (NTIS)

Increasing use of highly distributed applications, demand for faster data exchange, and highly parallel applications can push the limits of conventional external networking for IBM SP sites. In technical computing applications we have observed a growing u...

J. P. Navarro

1999-01-01

158

76 FR 22694 - SP 49 Pipeline LLC; Notice of Filing  

Federal Register 2010, 2011, 2012, 2013

...information to the Commission or to shippers. Effective November 12, 2010, SP 49 acquired a portion of Chevron Pipe Line Company's (``Chevron'') pipeline system and associated equipment, specifically the South Pass Block 49 Pipeline...

2011-04-22

159

Recall of m2000sp Instrument-Abbott Molecular, Inc.  

Center for Biologics Evaluation and Research (CBER)

... Recall of m2000sp Instrument-Abbott Molecular, Inc. DATE RECALL INITIATED: August 25, 2008. PRODUCT / LOT NUMBER: ... More results from www.fda.gov/biologicsbloodvaccines/safetyavailability/recalls

160

Characterization of Pseudomonas sp. BCNU 171 tolerant to organic solvents.  

PubMed

An organic solvent-tolerant bacterium, designated as Pseudomonas sp. BCNU 171, was isolated from industrial wastewater in Korea, on the basis of its ability to survive in the presence of benzene, toluene, propylbenzene and xylenes. Its tolerance limits were 8 mM in phenol, 20 mM in benzene and 60 M in toluene. The log P value of phenol was approximately 1.5, which indicates that Pseudomonas sp. BCNU 171 exhibits the highest tolerance to organic solvents. Pseudomonas sp. BCNU 171, a relative of P. putida, P. mosselii and P. moteillii based on phylogenetic analyses using 16S rRNA sequences, was designated as a new sp. that is tolerant to a wide spectrum of organic solvents, especially xylene isomers. These findings may facilitate the understanding of organic solvent tolerance in bacterial cells. PMID:18792055

Choi, Hye Jung; Kim, Seon-A; Kim, Dong Wan; Moon, Ja Young; Jeong, Yong Kee; Joo, Woo Hong

2008-12-01

161

55 - Food - Animal Products and Poultry Products, Beef Bos sp  

Center for Biologics Evaluation and Research (CBER)

Text VersionPage 1. 55 - Food - Animal Products and Poultry Products, Beef Bos sp Product: PubMed: Beef allergen; Beef allergy; Beef ... More results from www.fda.gov/downloads/biologicsbloodvaccines/allergenics

162

'PROCTOECES' SP. (TREMATODA: DIGENEA) IN THE AMERICAN OYSTER, 'CRASSOSTREA VIRGINICA'  

EPA Science Inventory

Histological examination of over 6,000 oysters, Crassostrea virginica, inhabiting northern Gulf Coast estuaries revealed unencysted junvenile and possible adult stages of digenetic trematode, Proctoeces sp., inhabiting the gonadal ducts of the mollusc. The morphology of the worm ...

163

The growth of Monoraphidium sp. and Scenedesmus sp. cells in the presence of thorium.  

PubMed

Toxicity of thorium by Monoraphidium sp. and Scenedesmus sp. was studied. Microalgal cultures were inoculated in ASM-1 medium in presence and absence of thorium. Its effect was monitored by direct counting on Fuchs-Rosenthal chamber and with software. The toxicity of thorium over the species was observed for concentrations over 50.0 mg/L. After 30 days, Monoraphidium cells decreased their concentration from 4.23 × 10(6) to 4.27 × 10(5) and 8.57 × 10(5) cells/mL, in the presence of 50.0 and 100.0 mg/L of thorium, respectively. Scenedesmus sp. cells were more resistant to thorium: for an initial cell concentration of 7.65 × 10(4) cells/mL it was observed a change to 5.25 × 10(5) and 5.12 × 10(5) cells/mL, in the presence of thorium at 50.0 and 100.0 mg/L, respectively. This is an indication that low concentrations of the radionuclide favored the growth, and that Scenedesmus cells are more resistant to thorium than Monoraphidium cells. The software used for comparison with direct count method proved to be useful for the improvement of accuracy of the results obtained, a decrease in the uncertainty and allowed recording of the data. The presence of thorium suggests that low concentrations have a positive effect on the growth, due to the presence of the nitrate, indicating its potential for ecotoxicological studies. PMID:22649297

de Queiroz, Juliana Cristina; Ferreira, Ana Cristina de Melo; da Costa, Antonio Carlos Augusto

2012-04-29

164

[Causative agent of human diphyllobothriasis--Diphyllobothrium klebanovskii sp. n].  

PubMed

A new cestode species, Diphyllobothrium klebanovskii sp. n., is described on the basis of experimental examination and morphological study of 51 specimens expelled from men in lower part of the Amur river and 18 specimens expelled from golden hamsters. The larval stage of this cestode is plerocercoid of type F, which is localized in muscles of Oncorhynchus keta and O. gorbuscha. D. klebanovskii sp. n. is a marine species, it differs from D. latum and other species of the genus Diphyllobothrium. PMID:3387122

Muratov, I V; Posokhov, P S

165

Microbial synthesis of silver nanoparticles by Bacillus sp  

Microsoft Academic Search

A silver resistant Bacillus sp. was isolated through exposure of an aqueous AgNO3 solution to the atmosphere. Silver nanoparticles were synthesized using these airborne bacteria (Bacillus sp.). Transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) analyses confirmed that silver nanoparticles\\u000a of 5–15 nm in size were deposited in the periplasmic space of the bacterial cells; a preferable cell surface location

Nalenthiran Pugazhenthiran; Sambandam Anandan; Govindarajan Kathiravan; Nyayiru Kannaian Udaya Prakash; Simon Crawford; Muthupandian Ashokkumar

2009-01-01

166

Lethrus (Lethrus) schneideri sp. n. (Coleoptera, Geotrupidae) from Greece  

PubMed Central

Abstract Lethrus (Lethrus) schneideri Král & Hillert, sp. n. from Thrace, Greece, is described. The new species is morphologically most similar and probably closely related to Lethrus (Lethrus) apterus (Laxmann, 1770) and Lethrus (Lethrus) ares Král, Rejsek & Schneider, 2001. Diagnostic characters (shape of mandibles, ventral mandible processes, pronotum and parameres) are illustrated. Character matrix for separation of males of the Lethrus species closely related to Lethrus schneideri Král & Hillert, sp. n. and geographic ranges for all species studied are mapped.

Kral, David; Hillert, Oliver; Drozova, Dana; Sipek, Petr

2013-01-01

167

Idiopathic Granulomatous Mastitis Associated with Corynebacterium Sp. Infection  

PubMed Central

Idiopathic granulomatous mastitis (IGM) is a rare inflammatory condition of the breast. The etiology and treatments options of IGM remain controversial. Previous case reports have suggested that Corynebacterium sp., a gram-positive bacillus endogenous to the skin, may be associated with IGM. In the present report, we describe the first case of IGM with a positive culture for Corynebacterium sp. reported in the United States.

Lee, Yun Sun; Balfour, John

2011-01-01

168

Mycetocola zhadangensis sp. nov., isolated from snow.  

PubMed

A Gram-stain-positive, aerobic, short rod-shaped bacterium, strain ZD1-4(T), was isolated from the Zhadang Glacier snow pit. The 16S rRNA gene sequence of the isolate showed highest similarity (98.8?%) to that of Mycetocola manganoxydans MB1-14(T). The major fatty acids of strain ZD1-4(T) were anteiso-C15?:?0, C16?:?0, C18?:?0 and anteiso-C17?:?0. It possessed diphosphatidylglycerol as one of the major polar lipids, and MK-10 and MK-11 as the predominant isoprenoid quinones. The DNA G+C content of strain ZD1-4(T) was 63.8±0.2 mol% (Tm). A number of phenotypic characteristics distinguished this bacterium from the type strains of other species of the genus Mycetocola. Moreover, the novel isolate showed only approximately 50?% DNA-DNA relatedness with M. manganoxydans MB1-14(T). According to these genotypic and phenotypic data, it is evident that strain ZD1-4(T) represents a novel species of the genus Mycetocola, for which the name Mycetocola zhadangensis sp. nov. is proposed. The type strain is ZD1-4(T) (?=?KACC 16570(T)?=?CGMCC 1.12042(T)). PMID:23524357

Shen, Liang; Liu, Yongqin; Yao, Tandong; Kang, Shichang; Wang, Yanan; Jiao, Nianzhi; Liu, Hongcan; Zhou, Yuguang; Xu, Baiqing; Liu, Xiaobo

2013-03-22

169

Aeromicrobium flavum sp. nov., isolated from air.  

PubMed

A Gram-positive, non-motile, facultatively anaerobic actinobacterium, designated TYLN1 T, was isolated from an air sample from the campus of Wuhan University, China, and subjected to a polyphasic taxonomic study. Strain TYLN1 T grew optimally at pH 7.0 and 30 degrees C. Analysis of 16S rRNA gene sequence similarities revealed that the organism belongs to the genus Aeromicrobium. The G+C content of the strain was 73.3 mol%. The cell-wall diamino acid was ll-2,6-diaminopimelic acid, the predominant menaquinones were MK-9(H4) and MK-8(H4) and the predominant fatty acid was C18:1 omega9c (68.38 %). These chemotaxonomic data also support the affiliation of strain TYLN1 T to the genus Aeromicrobium. The values for DNA-DNA hybridization between strain TYLN1 T and the phylogenetically closest relatives Aeromicrobium tamlense DSM 19087T and Aeromicrobium panaciterrae DSM 17939T were respectively 35 and 11.5 %. On the basis of the data from this study, the isolate represents a novel species, for which the name Aeromicrobium flavum sp. nov. is proposed. The type strain is TYLN1 T (=CCTCC AB 206046T =DSM 19355T). PMID:18676469

Tang, Yali; Zhou, Guoling; Zhang, Lei; Mao, Jing; Luo, Xuesong; Wang, Mengxi; Fang, Chengxiang

2008-08-01

170

Planobispora siamensis sp. nov., isolated from soil.  

PubMed

A novel actinomycete strain, A-T 4600(T), which developed cylindrical sporangia containing a longitudinal pair of motile spores forming singly or in bundles on short ramifications of the aerial mycelium, was isolated from soil collected from an evergreen forest in Thailand. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinones were MK-9(H2). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannoside, diphosphatidylglycerol and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17 : 1, C18 : 1, saturated C16 : 0, and C17 : 0. The G+C content of the DNA was 70.8 mol%. Phenotypic and chemotaxonomic analyses showed that the isolate had the typical characteristics of members of the genus Planobispora. Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belonged to the genus Planobispora but as a putative novel species. DNA-DNA relatedness values that differentiate the isolate from previously described members of the genus Planobispora were significantly below 70 %. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, it is proposed that the isolate represents a novel species, Planobispora siamensis sp. nov.; the type strain is A-T 4600(T) ( = BCC 39469(T) = NBRC 107568(T)). PMID:23291889

Ngaemthao, Wipaporn; Suriyachadkun, Chanwit; Chunhametha, Suwanee; Tamura, Tomohiko; Sanglier, Jean-Jacques

2013-01-04

171

Flavobacterium yanchengense sp. nov., isolated from soil.  

PubMed

A Gram-stain-negative, non-spore-forming, rod-shaped bacterial strain, hg(T), resembling members of the genus Flavobacterium, was isolated from soil, and subjected to a taxonomic study using a polyphasic approach. Strain hg(T) grew optimally at pH 7.0 and 30 °C in the presence of 1 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The DNA G+C content was 34 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain hg(T) belonged to the genus Flavobacterium. Levels of 16S rRNA gene sequence similarity between strain hg(T) and the type strains of species of the genus Flavobacterium were below 94.7 %. Strain hg(T) differed from phylogenetically related species of the genus Flavobacterium in several phenotypic characteristics. On the basis of phenotypic and phylogenetic distinctiveness, strain hg(T) (= CCTCC AB 2012099(T) = KACC 16855(T)) was classified in the genus Flavobacterium as the type strain of a novel species, for which the name Flavobacterium yanchengense sp. nov. is proposed. PMID:23315414

Hu, Gang; Zhang, Jun; Yang, Guiqin; Li, Yang-Yang; Guan, Yi-Ting; Wang, Jun; Li, Shun-Peng; Hong, Qing

2013-01-11

172

Mineralization of phenanthrene by a Mycobacterium sp.  

PubMed Central

A Mycobacterium sp., designated strain BG1, able to utilize the polycyclic aromatic hydrocarbon phenanthrene as the sole carbon and energy source was isolated from estuarine sediment following enrichment with the hydrocarbon. Unlike other phenanthrene degraders, this bacterium degraded phenanthrene via 1-hydroxy-2-naphthoic acid without accumulating this or other aromatic intermediates, as shown by high-performance liquid chromatography. Degradation proceeded via meta cleavage of protocatechuic acid. Different nonionic surfactants (Tween compounds) solubilized the phenanthrene to different degrees and enhanced phenanthrene utilization. The order of enhancement, however, did not correlate perfectly with increased solubility, suggesting physiological as well as physicochemical effects of the surfactants. Plasmids of approximately 21, 58, and 77 megadaltons were detected in cells grown with phenanthrene but not in those which, after growth on nutrient media, lost the phenanthrene-degrading phenotype. Given that plasmid-mediated degradations of aromatic hydrocarbons generally occur via meta cleavages, it is of interest that the addition of pyruvate, a product of meta cleavage, supported rapid mineralization of phenanthrene in broth culture; succinate, a product of ortho cleavage, supported growth but completely repressed the utilization of phenanthrene. The involvement of plasmids may have given rise to the unusual degradation pattern that was observed. Images

Guerin, W F; Jones, G E

1988-01-01

173

Actinoplanes siamensis sp. nov., isolated from soil.  

PubMed

A Gram-positive filamentous bacterial strain that developed large campanulate sporangia at the ends of sporangiophores on substrate mycelium was isolated from bamboo forest soil in Thailand. According to the results of a polyphasic taxonomic study, our isolate had typical characteristics of members of the genus Actinoplanes. The 16S rRNA gene sequence analysis also indicated that strain A-T 6646(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes liguriensis DSM 43865(T) (97.61 %) and Actinoplanes octamycinicus NBRC 14524(T) (97.52 %). The DNA-DNA relatedness values, which differentiate the new strain from the most closely related species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained xylose and arabinose. The predominant menaquinone was MK-9(H4). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and anteiso-C15 : 0. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, the isolate is proposed to represent a novel species to be named Actinoplanes siamensis sp. nov. The type strain is A-T 6646(T) (= BCC 46194(T) = NBRC 109076(T)). PMID:23396720

Suriyachadkun, Chanwit; Ngaemthao, Wipaporn; Chunhametha, Suwanee; Thawai, Chitti; Sanglier, Jean-Jacques

2013-02-08

174

Lysobacter ximonensis sp. nov., isolated from soil.  

PubMed

A bacterial isolate, strain XM415(T), obtained from soil of Tibet in China was characterized using a polyphasic taxonomic approach. Strain XM415(T) was aerobic, Gram-negative, gliding, rod-shaped, oxidase-negative and catalase-positive. Strain XM415(T) showed the highest 16S rRNA gene sequence similarity with Lysobacter niastensis GH41-7(T) (96.0 %). Ubiquinone Q-8 and branched fatty acids, such as iso-C(16 : 0) (24.0 %), iso-C(15 : 0) (22.6 %), iso-C(17 : 1)omega9c (6.7 %), iso-C(14 : 0) (6.1 %) and iso-C(11 : 0) 3-OH (5.2 %), were predominant in strain XM415(T) as well as in all type strains of recognized Lysobacter species. The DNA G+C content of XM415(T) was 63.5 mol%. The genotypic and phenotypic data show that strain XM415(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter ximonensis sp. nov. is proposed. The type strain is XM415(T) (=CCTCC AB 207091(T) =NRRL B-51263(T)). PMID:19329607

Wang, Yang; Dai, Jun; Zhang, Lei; Luo, Xuesong; Li, Yaowei; Chen, Gong; Tang, Yali; Meng, Ya; Fang, Chengxiang

2009-04-01

175

Lysobacter dokdonensis sp. nov., isolated from soil.  

PubMed

A Gram-negative, non-motile, rod-shaped bacterial strain, DS-58(T), was isolated from a soil sample from Dokdo, an island of Korea, and its taxonomic position was investigated. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-58(T) fell within the family Xanthomonadaceae. The isolate showed 96.9 % 16S rRNA gene sequence similarity with its closest phylogenetic neighbour, Lysobacter niastensis GH41-7(T), and 93.4-95.7 % 16S rRNA gene sequence similarity with other members of the genus Lysobacter. Strain DS-58(T) contained Q-8 as the predominant ubiquinone and iso-C(16 : 0), iso-C(15 : 0) and iso-C(17 : 1)?9c as the major fatty acids. The DNA G+C content was 68.1 mol%. Strain DS-58(T) could be distinguished phenotypically from type strains of closely related species of the genus Lysobacter and phylogenetically from all members of the genus Lysobacter. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DS-58(T) is considered to represent a novel species of the genus Lysobacter, for which the name Lysobacter dokdonensis sp. nov. is proposed. The type strain is DS-58(T) (?=?KCTC 12822(T) ?=?DSM 17958(T)). PMID:20525815

Oh, Ki-Hoon; Kang, So-Jung; Jung, Yong-Taek; Oh, Tae-Kwang; Yoon, Jung-Hoon

2010-06-04

176

Flavobacterium fontis sp. nov., isolated from freshwater.  

PubMed

A taxonomic study was carried out on a novel bacterial strain, designated MIC3010(T), which was isolated from a freshwater pond in Jeonju, Republic of Korea. Cells of the isolate were Gram-stain-negative, rod-shaped and non-motile. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the family Flavobacteriaceae, with Flavobacterium haoranii LQY-7(T) as its closest relative, with a similarity of 94.2?%. The predominant fatty acids of strain MIC3010(T) were iso-C15?:?1 G, iso-C15?:?0 and iso-C17?:?0 3-OH. The polar lipid profile of strain MIC3010(T) revealed the presence of phosphatidylethanolamine (PE) and one unidentified lipid (L1) as major components. In addition, two aminolipids (AL1, AL2) and one glycolipid were present in small amounts. The DNA G+C content of the strain was 41.0 mol%. The strain contained MK-6 as the major quinone and sym-homospermidine as the predominant polyamine. On the basis of the evidence presented, it is concluded that strain MIC3010(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium fontis sp. nov. is proposed. The type strain is MIC3010(T) (?=?KACC 16593(T)?=?JCM 18212(T)). PMID:22904217

Chun, Jeesun; Kang, Ji Young; Jahng, Kwang Yeop

2012-08-17

177

Detection of Plasmodium sp. in capybara.  

PubMed

In the present study, we have microscopically and molecularly surveyed blood samples from 11 captive capybaras (Hydrochaeris hydrochaeris) from the Sanctuary Zoo for Plasmodium sp. infection. One animal presented positive on blood smear by light microscopy. Polymerase chain reaction was carried out accordingly using a nested genus-specific protocol, which uses oligonucleotides from conserved sequences flanking a variable sequence region in the small subunit ribosomal RNA (ssrRNA) of all Plasmodium organisms. This revealed three positive animals. Products from two samples were purified and sequenced. The results showed less than 1% divergence between the two capybara sequences. When compared with GenBank sequences, a 55% similarity was obtained to Toxoplasma gondii and a higher similarity (73-77.2%) was found to ssrRNAs from Plasmodium species that infect reptile, avian, rodents, and human beings. The most similar Plasmodium sequence was from Plasmodium mexicanum that infects lizards of North America, where around 78% identity was found. This work is the first report of Plasmodium in capybaras, and due to the low similarity with other Plasmodium species, we suggest it is a new species, which, in the future could be denominated "Plasmodium hydrochaeri". PMID:19411142

dos Santos, Leonilda Correia; Curotto, Sandra Mara Rotter; de Moraes, Wanderlei; Cubas, Zalmir Silvino; Costa-Nascimento, Maria de Jesus; de Barros Filho, Ivan Roque; Biondo, Alexander Welker; Kirchgatter, Karin

2009-04-05

178

Candida chanthaburiensis sp. nov., Candida kungkrabaensis sp. nov. and Candida suratensis sp. nov., three novel yeast species from decaying plant materials submerged in water of mangrove forests.  

PubMed

In a taxonomic study of yeasts isolated from decaying plant materials submerged in water of mangrove forests in Thailand, three strains isolated from tree bark (EM33(T)), a fallen leaf (EM40(T)) and a detached branch (SM56(T)) were found to represent three novel yeast species. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene, and the phylogenetic analysis, the three strains were assigned as three novel Candida species. They were named as Candida chanthaburiensis sp. nov. (type strain EM33(T) = BCC 23057(T) = NBRC 102176(T) = CBS 10926(T)), Candida kungkrabaensis sp. nov. (type strain EM40(T) = BCC 23060(T) = NBRC 102179(T) = CBS 10927(T)), and Candida suratensis sp. nov. (type strain SM56(T) = BCC 25961(T) = NBRC 103858(T) = CBS 10928(T)). PMID:20467812

Limtong, Savitree; Yongmanitchai, Wichien

2010-05-14

179

Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere.  

PubMed

Three novel bacterial strains, PVAS-1(T), B3W22(T) and B8W22(T), were isolated from cryotubes used to collect air samples at altitudes of between 27 and 41 km. Based on phenotypic characteristics, chemotaxonomic features, DNA-DNA hybridization with the nearest phylogenetic neighbours and phylogenetic analysis based on partial 16S rRNA gene sequences (PVAS-1(T), 1196 nt; B3W22(T), 1541 nt; B8W22(T), 1533 nt), the three strains were identified as representing novel species, and the names proposed are Janibacter hoylei sp. nov. (type strain PVAS-1(T) =MTCC 8307(T) =DSM 21601(T) =CCUG 56714(T)), Bacillus isronensis sp. nov. (type strain B3W22(T) =MTCC 7902(T) =JCM 13838(T)) and Bacillus aryabhattai sp. nov. (type strain B8W22(T) =MTCC 7755(T) =JCM 13839(T)). PMID:19643890

Shivaji, S; Chaturvedi, Preeti; Begum, Zareena; Pindi, Pavan Kumar; Manorama, R; Padmanaban, D Ananth; Shouche, Yogesh S; Pawar, Shrikant; Vaishampayan, Parag; Dutt, C B S; Datta, G N; Manchanda, R K; Rao, U R; Bhargava, P M; Narlikar, J V

2009-07-30

180

Molecular and functional properties of lung SP cells.  

PubMed

Previous analysis of lung injury and repair has provided evidence for region-specific stem cells that maintain proximal and distal epithelial compartments. However, redundant expression of lineage markers by cells at several levels of the stem cell hierarchy has complicated phenotypic and functional characterization of clonogenic airway cells. Based on the demonstration that rapid efflux of the DNA dye Hoechst 33342 can be used to prospectively purify long-term repopulating hematopoietic stem cells, we hypothesized that lung cells with similar biochemical properties would be enriched for clonogenic progenitors. We demonstrate that Hoechst-dim side population (SP) cells isolated from proximal and distal compartments of the mouse lung were relatively small and agranular, exhibited low red and green autofluorescence, and that the SP fraction was highly enriched in clonogenic cells. Quantitative RT-PCR indicated that vimentin mRNA was enriched and that epithelial markers were depleted in these preparations of SP cells. Bleomycin exposure was associated with decreased clonogenicity among alveolar SP and suggested that SP cell function was compromised under profibrotic conditions. We conclude that the SP phenotype is common to clonogenic cells at multiple airway locations and suggest that Hoechst efflux is a property of cells expressing a wound-repair phenotype. PMID:17142352

Reynolds, Susan D; Shen, Hongmei; Reynolds, Paul R; Betsuyaku, Tomoko; Pilewski, Joseph M; Gambelli, Federica; Di Giuseppe, Michelangelo; DeGuiseppe, Michelangelo; Ortiz, Luis A; Stripp, Barry R

2006-12-01

181

Pd(II)-catalyzed cross-coupling of sp3 C-H Bonds with sp2 and sp3 boronic acids using air as the oxidant.  

PubMed

O-Methyl hydroxamic acids, readily available from carboxylic acids, are found to be extremely reactive for beta-C-H activation by Pd(OAc)2. This reactivity is exploited to develop the first example of cross-coupling sp3 C-H bonds with sp3 boronic acids. Air was shown to be a suitable stoichiometric oxidant for the catalytic oxidative coupling reaction. A biologically active natural product is readily converted to its novel analogues through this coupling reaction. PMID:18479089

Wang, Dong-Hui; Wasa, Masayuki; Giri, Ramesh; Yu, Jin-Quan

2008-05-14

182

Biodegradation of phenol by using free and immobilized cells of Acinetobacter sp. XA05 and Sphingomonas sp. FG03  

Microsoft Academic Search

Strain XA05 and FG03 with high biodegradation activity of phenol were isolated from the activated sludge and phenol-contaminated soils in Northwest of China, respectively. DNA sequencing and homologous analysis of 16s rRNA gene identified that XA05 belonged to an Acinetobacter sp. and FG03 was closely related to the Sphingomonas sp. Strain XA05 and FG03 were mixed at the ratio of

Y. J. Liu; A. N. Zhang; X. C. Wang

2009-01-01

183

In Vitro Antimicrobial Potential of the Lichen Parmotrema sp. Extracts against Various Pathogens  

PubMed Central

Objective(s): The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. The perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. Lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. This study was aimed to explore in vitro antimicrobial activity of lichen Parmotrema sp. Material and Methods: The methanol and aqueous extracts of lichen Parmotrema sp. was extracted using Soxhlet extractor. Antibiotic assessment of methanol and aqueous extracts was done against eight bacterial (Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Shigella sp., Enterococci faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae,) clinical pathogens and five plant pathogenic fungal strains (Aspergillus terreus strain JAS1, Scedosporium sp. JAS1, Ganoderma sp. JAS4, Candida tropicalis and Fusarium sp.) by Kirby-Bauer method. Results: The methanol lichen Parmotrema sp. extract inhibited all the test organisms. The highest antibacterial activity was found against Pseudomonas aeruginosa and Staphylococcus aureus. The weakest activity was manifested in Salmonella sp. and Scedosporium sp. JAS1. Strong antifungal effect was found against Ganoderma sp. JAS4 and Fusarium sp. The aqueous lichen Parmotrema sp. extract revealed neither antibacterial nor antifungal activity. Conclusion: The present study shows that tested lichen Parmotrema sp. extracts demonstrated a strong antimicrobial effect. That suggests the active components from methanol extracts of the investigated lichen Parmotrema sp. can be used as natural antimicrobial agent against pathogens.

Chauhan, Ritika; Abraham, Jayanthi

2013-01-01

184

In Vitro Antimicrobial Potential of the Lichen Parmotrema sp. Extracts against Various Pathogens.  

PubMed

Objective(s): The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. The perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. Lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. This study was aimed to explore in vitro antimicrobial activity of lichen Parmotrema sp. Material and Methods: The methanol and aqueous extracts of lichen Parmotrema sp. was extracted using Soxhlet extractor. Antibiotic assessment of methanol and aqueous extracts was done against eight bacterial (Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Shigella sp., Enterococci faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae,) clinical pathogens and five plant pathogenic fungal strains (Aspergillus terreus strain JAS1, Scedosporium sp. JAS1, Ganoderma sp. JAS4, Candida tropicalis and Fusarium sp.) by Kirby-Bauer method. Results: The methanol lichen Parmotrema sp. extract inhibited all the test organisms. The highest antibacterial activity was found against Pseudomonas aeruginosa and Staphylococcus aureus. The weakest activity was manifested in Salmonella sp. and Scedosporium sp. JAS1. Strong antifungal effect was found against Ganoderma sp. JAS4 and Fusarium sp. The aqueous lichen Parmotrema sp. extract revealed neither antibacterial nor antifungal activity. Conclusion: The present study shows that tested lichen Parmotrema sp. extracts demonstrated a strong antimicrobial effect. That suggests the active components from methanol extracts of the investigated lichen Parmotrema sp. can be used as natural antimicrobial agent against pathogens. PMID:23997920

Chauhan, Ritika; Abraham, Jayanthi

2013-07-01

185

Aminobacter ciceronei sp. nov. and Aminobacter lissarensis sp. nov., isolated from various terrestrial environments.  

PubMed

The bacterial strains IMB-1(T) and CC495(T), which are capable of growth on methyl chloride (CH(3)Cl, chloromethane) and methyl bromide (CH(3)Br, bromomethane), were isolated from agricultural soil in California fumigated with CH(3)Br, and woodland soil in Northern Ireland, respectively. Two pesticide-/herbicide-degrading bacteria, strains ER2 and C147, were isolated from agricultural soil in Canada. Strain ER2 degrades N-methyl carbamate insecticides, and strain C147 degrades triazine herbicides widely used in agriculture. On the basis of their morphological, physiological and genotypic characteristics, these four strains are considered to represent two novel species of the genus Aminobacter, for which the names Aminobacter ciceronei sp. nov. (type strain IMB-1(T)=ATCC 202197(T)=CIP 108660(T)=CCUG 50580(T); strains ER2 and C147) and Aminobacter lissarensis sp. nov. (type strain CC495(T)=NCIMB 13798(T)=CIP 108661(T)=CCUG 50579(T)) are proposed. PMID:16166673

McDonald, Ian R; Kämpfer, Peter; Topp, Ed; Warner, Karen L; Cox, Michael J; Hancock, Tracy L Connell; Miller, Laurence G; Larkin, Michael J; Ducrocq, Veronique; Coulter, Catherine; Harper, David B; Murrell, J Colin; Oremland, Ronald S

2005-09-01

186

Trichuris sp. and Strongyloides sp. Infections in a Free-Ranging Baboon Colony  

PubMed Central

We conducted cross-sectional surveys of parasites infecting a large free-living colony of baboons at the Southwest National Primate Research Center in San Antonio in October 2003 and April 2004, immediately before and 6 mo after treatment with ivermectin. Trichuris sp. was the predominant species present infecting 79 and 69% of individual animals in the 2 surveys, with fecal egg counts (FEC) of up to 60,200 eggs per g (epg) (mean = 1,235 in Oct 2003 and 1,256 in Apr 2004). Prevalence remained fairly stable across age groups, while intensity was highest in animals <1- or >15-yr-old, in contrast to patterns observed in humans where school age children show the heaviest infections. Strongyloides sp. was also identified, but the species remains uncertain. Small subunit ribosomal DNA sequences differed from published sequences of S. fuelleborni at multiple sites but resided in a monophyletic group with other Strongyloides species with 92% bootstrap support. This may reflect a recent acquisition from a local host, or that the published sequence of S. fuelleborni is incorrect. Widespread infections with 2 nematode genera in a free-ranging baboon colony that are an important source of morbidity in human populations provides a useful model system for work on the epidemiology, control, pathology, and genetics of these parasites in a host species that is physiologically, immunologically and genetically similar to humans.

Anderson, J.; Upadhayay, R.; Sudimack, D.; Nair, S.; Leland, M.; Williams, J. T.; Anderson, T. J. C.

2013-01-01

187

Cohnella thermotolerans gen. nov., sp. nov., and classification of 'Paenibacillus hongkongensis' as Cohnella hongkongensis sp. nov.  

PubMed

A Gram-positive, rod-shaped, endospore-forming organism, strain CCUG 47242T, was isolated from a sample of industrial starch production in Sweden. 16S rRNA gene sequence analysis demonstrated that this isolate was moderately related to species of the genus Paenibacillus, with <94.4 % sequence similarity to all other hitherto described Paenibacillus species. Strain CCUG 47242T showed the greatest sequence similarity (96.5 %) to 'Paenibacillus hongkongensis' HKU3, a strain with a name that has not yet been validly published. Chemotaxonomic data [major menaquinone, MK-7 (98 %); major polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, two unknown phospholipids, four unknown aminophospholipids; major fatty acids, iso-C16 : 0 and anteiso-C15 : 0] showed some significant differences when compared with the type species of the genus Paenibacillus, Paenibacillus polymyxa. Physiological and biochemical tests allowed clear phenotypic differentiation of strain CCUG 47242T from strain HKU3. On the basis of 16S rRNA gene sequence analysis, in combination with chemotaxonomic data, strains CCUG 47242T and HKU3 represent two novel species of a new genus of endospore-forming bacteria for which we propose the names Cohnella thermotolerans gen. nov., sp. nov. (type strain CCUG 47242T = CIP 108492T = DSM 17683T) and Cohnella hongkongensis sp. nov. (type strain HKU3T = CCUG 49571T = CIP 107898T = DSM 17642T). PMID:16585694

Kämpfer, Peter; Rosselló-Mora, Ramon; Falsen, Enevold; Busse, Hans-Jürgen; Tindall, Brian J

2006-04-01

188

Nocardia goodfellowii sp. nov. and Nocardia thraciensis sp. nov., isolated from soil.  

PubMed

The taxonomic position of two soil actinomycetes, strains A2012(T) and A2019(T), isolated from Turkish soils, was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that the strains belonged to the family Nocardiaceae. Strains A2012(T) and A2019(T) were most closely related to Nocardia caishijiensis DSM 44831(T) (98.9 %) and Nocardia mexicana CIP 108295(T) (98.6 %), respectively; similarity to other type strains of the genus Nocardia ranged from 96.9 to 97.9 %. However, DNA-DNA relatedness and phenotypic data demonstrated that strains A2012(T) and A2019(T) could be clearly distinguished from members of the most closely related Nocardia species. It is evident from the genotypic and phenotypic data that the two isolates represent two novel species of the genus Nocardia. It is proposed, therefore, that strains A2012(T) and A2019(T) be classified in the genus Nocardia as representatives of Nocardia goodfellowii sp. nov. (type strain A2012(T) = DSM 45516(T) = NRRL B-24833(T) = KCTC 19986(T)) and Nocardia thraciensis sp. nov. (type strain A2019(T) = DSM 45517(T) = NRRL B-24834(T) = KCTC 19985(T)), respectively. PMID:21742817

Sazak, Anil; Sahin, Nevzat; Camas, Mustafa

2011-07-08

189

Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces.  

PubMed

Six strains (CB7(T), CB18, CB23, CB26, CB28 and CB35(T)) were isolated from human faeces. Based on phylogenetic analysis, phenotypic characteristics, cellular fatty acid profiles and menaquinone profiles, these strains could be included within the genus Prevotella and made up two clusters. 16S rRNA gene sequence analysis indicated that five strains were most closely related to Prevotella veroralis, sharing about 92 % sequence similarity; the remaining strain was most closely related to Prevotella shahii, sharing about 90 % sequence similarity. All six strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. The cellular fatty acid compositions of the six strains differed significantly from those of other Prevotella species. Five strains (CB7(T), CB18, CB23, CB26 and CB28) contained dimethyl acetals and the major menaquinones of these strains were MK-11, MK-12 and MK-13. The major menaquinones of CB35(T) were MK-12 and MK-13. Based on phenotypic and phylogenetic findings, two novel species, Prevotella copri sp. nov. and Prevotella stercorea sp. nov., are proposed, representing the two different strain clusters. The DNA G+C contents of strains CB7(T) and CB35(T) were 45.3 and 48.2 mol%, respectively. The type strains of P. copri and P. stercorea are CB7(T) (=JCM 13464(T)=DSM 18205(T)) and CB35(T) (=JCM 13469(T)=DSM 18206(T)), respectively. PMID:17473237

Hayashi, Hidenori; Shibata, Kensaku; Sakamoto, Mitsuo; Tomita, Shinichi; Benno, Yoshimi

2007-05-01

190

Cellulomonas soli sp. nov. and Cellulomonas oligotrophica sp. nov., isolated from soil.  

PubMed

Two novel bacterial strains, designated Kc1(T) and Kc5(T), were isolated from soil in Japan. Cells of the novel strains were Gram-reaction-positive, aerobic or facultatively anaerobic, motile rods. Phylogenetic analyses based on 16S rRNA gene sequences indicated that both strains belonged to the genus Cellulomonas. The 16S rRNA gene sequences of strains Kc1(T) and Kc5(T) showed closest similarity to that of Cellulomonas terrae DB5(T) (98.1 % and 98.4 % similarity, respectively), and the 16S rRNA gene similarity between the two novel strains was 97.8 %. In both strains, the major menaquinone was MK-9(H(4)), the predominant polar lipids were diphosphatidylglycerol and phosphatidylinositol mannosides, and the peptidoglycan contained ornithine and glutamic acid. Cell-wall sugars were identified as rhamnose, galactose and mannose in strain Kc1(T) and rhamnose and glucose in strain Kc5(T). The DNA G+C contents of strains Kc1(T) and Kc5(T) were 73.6 mol% and 75.8 mol%, respectively. Based on the chemotaxonomic and physiological data and the results of DNA-DNA hybridizations, the two strains represent two novel species within the genus Cellulomonas, for which the names Cellulomonas soli sp. nov. (type strain Kc1(T) =DSM 24484(T) =JCM 17535(T)) and Cellulomonas oligotrophica sp. nov. (type strain Kc5(T) =DSM 24482(T) =JCM 17534(T)) are proposed. PMID:22328604

Hatayama, Kouta; Esaki, Kouji; Ide, Teruhiko

2012-02-10

191

Treponema berlinense sp. nov. and Treponema porcinum sp. nov., novel spirochaetes isolated from porcine faeces.  

PubMed

Limit-dilution procedures were used to isolate seven, helically coiled bacterial strains from faeces of swine that constituted two unidentified taxa. Comparative 16S rRNA gene sequence analysis showed highest similarity values with species of the genus Treponema indicating that the isolates are members of this genus. Strain 7CPL208(T), as well as five further isolates, and 14V28(T) displayed the highest 16S rRNA gene sequence similarities with Treponema pectinovorum ATCC 33768(T) (92.3%) and Treponema parvum OMZ 833(T) (89.9%), respectively. Polar lipid profiles distinguished 7CPL208(T) and 14V28(T) from each other as well as from related species. Based on their phenotypic and genotypic distinctiveness, strains 7CPL208(T) and 14V28(T) are suggested to represent two novel species of the genus Treponema, for which the names Treponema berlinense sp. nov. and Treponema porcinum sp. nov. are proposed. The type strain for Treponema berlinense is 7CPL208(T) (=ATCC BAA-909(T)=CIP 108244(T)=JCM 12341(T)) and for Treponema porcinum 14V28(T) (=ATCC BAA-908(T)=CIP 108245(T)=JCM 12342(T)). PMID:16014500

Nordhoff, Marcel; Taras, David; Macha, Moritz; Tedin, Karsten; Busse, Hans-Jürgen; Wieler, Lothar H

2005-07-01

192

Glycomyces scopariae sp. nov. and Glycomyces mayteni sp. nov., isolated from medicinal plants in China.  

PubMed

Two actinomycete strains, designated YIM 56256(T) and YIM 61331(T), were isolated from the roots of Scoparia dulcis and Maytenus austroyunnanensis, two Chinese medicinal plants, and their taxonomic status was established based on a polyphasic investigation. The organisms were found to have chemical and morphological markers typical of members of the genus Glycomyces. 16S rRNA gene sequence analysis showed that they were closely related to each other and to Glycomyces sambucus E71(T). A battery of physiological characteristics and levels of DNA-DNA relatedness indicated that strains YIM 56256(T) and YIM 61331(T) represent two novel species, clearly different from the related known Glycomyces species. On the basis of the data presented, it is evident that each of these strains represents a novel species of the genus Glycomyces, for which the names Glycomyces scopariae sp. nov. (type strain YIM 56256(T) =KCTC 19158(T) =DSM 44968(T)) and Glycomyces mayteni sp. nov. (type strain YIM 61331(T) =KCTC 19527(T) =CCTCC AA 208004(T)) are proposed. PMID:19406786

Qin, Sheng; Chen, Hua-Hong; Klenk, Hans-Peter; Zhao, Guo-Zhen; Li, Jie; Xu, Li-Hua; Li, Wen-Jun

2009-05-01

193

Aminobacter ciceronei sp. nov. and Aminobacter lissarensis sp. nov., isolated from various terrestrial environments  

USGS Publications Warehouse

The bacterial strains IMB-1T and CC495T, which are capable of growth on methyl chloride (CH3Cl, chloromethane) and methyl bromide (CH3Br, bromomethane), were isolated from agricultural soil in California fumigated with CH3Br, and woodland soil in Northern Ireland, respectively. Two pesticide- /herbicide-degrading bacteria, strains ER2 and C147, were isolated from agricultural soil in Canada. Strain ER2 degrades N-methyl carbamate insecticides, and strain C147 degrades triazine herbicides widely used in agriculture. On the basis of their morphological, physiological and genotypic characteristics, these four strains are considered to represent two novel species of the genus Aminobacter, for which the names Aminobacter ciceronei sp. nov. (type strain IMB-1T=ATCC 202197T=CIP 108660T=CCUG 50580T; strains ER2 and C147) and Aminobacter lissarensis sp. nov. (type strain CC495T=NCIMB 13798T=CIP 108661T=CCUG 50579T) are proposed. ?? 2005 IUMS.

McDonald, I. R.; Kampfer, P.; Topp, E.; Warner, K. L.; Cox, M. J.; Connell, Hancock, T. L.; Miller, L. G.; Larkin, M. J.; Ducrocq, V.; Coulter, C.; Harper, D. B.; Murrell, J. C.; Oremland, R. S.

2005-01-01

194

Streptomyces chlorus sp. nov. and Streptomyces viridis sp. nov., isolated from soil.  

PubMed

Two actinomycete strains, BK125(T) and BK199(T), isolated from a hay meadow soil sample were investigated to determine their taxonomic position using a polyphasic approach. The isolates produced greenish-yellow and light green aerial mycelium on oatmeal agar, respectively. They contained anteiso-C15?:?0, iso-C15?:?0 and C16?:?0 as the major fatty acids, and MK-9 (H6) and MK-9 (H8) as the predominant isoprenoid quinones. Phylogenetic analysis of the 16S rRNA gene sequences showed that the isolates formed distinct phyletic lines towards the periphery of the Streptomyces prasinus subclade. Analysis of DNA-DNA relatedness between the two isolates showed that they belonged to different genomic species. The organisms were also distinguished from one another and from type strains of species classified in the S. prasinus subclade using a combination of genotypic and phenotypic properties. On the basis of these data, it is proposed that the isolates be assigned to the genus Streptomyces as Streptomyces chlorus sp. nov. and Streptomyces viridis sp. nov. with isolates BK125(T) (?=?KACC 20902(T)?=?CGMCC 4.5798(T)) and BK199(T) (?=?KACC 21003(T)?=?CGMCC 4.6824(T)) as the respective type strains. PMID:22922536

Kim, Byung-Yong; Rong, Xiaoying; Zucchi, Tiago D; Huang, Ying; Goodfellow, Michael

2012-08-24

195

Ecotoxicity study of titania (TiO?) NPs on two microalgae species: Scenedesmus sp. and Chlorella sp.  

PubMed

In view of their increasing commercial applications metal oxide NPs like titania have elevated chances of entry to the environment. The ecotoxicity analyses are required to assess their environmental risks. The present work aims to demonstrate the effect of titania NPs on microalgae isolated from freshwater environment (Scenedesmus sp. and Chlorella sp.). The growth inhibitory effect of titania NPs was observed for both the species (72 h EC?? value, 16.12 mg/L for Chlorella sp.; 21.2 mg/L for Scenedesmus sp.). Bulk micron-sized titania also showed toxicity though to a lesser extent (72 h EC?? value, 35.50mg/L for Chlorella sp.; 44.40 mg/L for Scenedesmus sp.). A concentration dependent decrease in chlorophyll content was observed in the treated cells compared to the untreated ones, more effect being notable in case of NPs. Preliminary results based on FT-IR studies and microscopic images suggest interaction of the NPs with the cell surface. PMID:21481931

Sadiq, I Mohammed; Dalai, Swayamprava; Chandrasekaran, N; Mukherjee, A

2011-04-08

196

Aeromicrobium ponti sp. nov., isolated from seawater.  

PubMed

A novel actinomycete strain, designated HSW-1T, was isolated from seawater collected from Hwasun Beach on the coast of Jeju Island, Republic of Korea. The cells were aerobic, Gram-positive, oxidase-negative, catalase-positive, non-motile rods. The colonies were circular, smooth, convex and yellow in colour. The cell-wall peptidoglycan of this organism contained ll-diaminopimelic acid as the diagnostic diamino acid. The polar lipids included phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and three unknown phospholipids. The predominant menaquinone was MK-9(H4). The major fatty acids were C18 : 1 omega 9c, C16 : 0, C16 : 0 2-OH and 10-methyl C18 : 0. The DNA G+C content was 74.0 mol%. A neighbour-joining tree based on 16S rRNA gene sequences showed that this organism falls within the radiation of the genus Aeromicrobium. The closest phylogenetic neighbours were the type strains of Aeromicrobium erythreum (98.2 % 16S rRNA gene sequence similarity), Aeromicrobium alkaliterrae (97.9 %), Aeromicrobium marinum (97.3 %) and Aeromicrobium fastidiosum (97.0 %). The DNA-DNA relatedness values between the novel isolate and its closest relative, A. erythreum DSM 8599T, ranged between 32 and 36 %. On the basis of the phenotypic and DNA-DNA hybridization data, strain HSW-1T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium ponti sp. nov. is proposed. The type strain is HSW-1T (=DSM 19178T=KACC 20565T). PMID:18398207

Lee, Dong Wan; Lee, Soon Dong

2008-04-01

197

Genetically confirmed Fasciola hepatogigantica n.sp.  

PubMed

Identification of liver fluke species cannot be achieved by clinical, pathological, coprological or immunological methods. However, the differential diagnosis between F. hepatica and F. gigantica infection is very important because of their different pathological manifestations. Moreover, in countries where the two species co-exist, morphologically intermediate forms were reported. The present study aimed to identify these forms by the use of molecular characterization of DNA sequence. Based on morphometric criteria, adults of Fasciola hepatica, F. gigantica and intermediate forms were collected from naturally infected sheep and cattle from various regions of Sohag Governorate. A simple and rapid new method (QIAamp DNA Mini Kit) was used to isolate DNA from the worms and their RELP patterns were obtained after digestion of the PCR products with AvalI restriction enzymes. The result of a regular PCR experiment for the amplification of the selected 28S rDNA fragment with the designed primer set yielded identical 618- bp-long PCR products for the three types of Fasciola where the RFLP profile obtained from F. hepatica revealed four fragments of 628, 575, 165 and 95 bp, and F. gigantica generated three fragments corresponding to 628, 358 and 300 bp fragments whereas the intermediate forms revealed four fragments of 628, 541, 358 and 300 bp, which were similar to those ofF. gigantica but with a distinctive fragment of 541. These results confirmed that three species are present in our locality: F. hepatica, F. gigantica and an intermediate form which was named F. hepatogigantica n.sp. on basis of having few morphometric characters from F. hepatica (length and pattern of uterine coils) but genetically they were more related to F. gigantica. PMID:23697013

Khalifa, Refaat M A; El-Hady, Hanaa A; Omran, Eman K; Ahmed, Noha S

2013-04-01

198

Pseudomonas punonensis sp. nov., isolated from straw.  

PubMed

During a study of the 'tunta' (frozen-dry potato) production process in Peru, a bacterial strain, LMT03(T), was isolated from the straw grass in which the potatoes are dried. This strain was classified into the genus Pseudomonas on the basis of the 16S rRNA gene sequence analysis, and is most closely related to Pseudomonas argentinensis CH01(T) with 99.3?% identity in this gene and 96?%, 92?% and 86?% identities in rpoB, rpoD and gyrB genes, respectively. Strain LMT03(T) has a single polar flagellum, like other related yellow-pigment-producing pseudomonads. The major quinone is Q-9. The major fatty acids are C18?:?1?7c in summed feature 8 (40.82?%), C16?:?1?6c/C16?:?1?6c in summed feature 3 (23.72?%) and C16?:?0 (15.20?%). The strain produces oxidase but it does not produce gelatinase, indole, urease, arginine dihydrolase or ?-galactosidase. Catalase production was very weak after 28 and 48 h incubation on nutrient agar medium. Nitrate reduction is negative. It does not hydrolyse aesculin. The DNA G+C content is 57.8 mol%. DNA-DNA hybridization results showed lower than 52?% relatedness with respect to the type strain of P. argentinensis, CH01(T). These results, together with other phenotypic characteristics, support the definition of a novel species within the genus Pseudomonas, for which the name Pseudomonas punonensis sp. nov. is proposed. The type strain is LMT03(T) (?=?LMG 26839(T)?=?CECT 8089(T)). PMID:23002045

Ramos, Elena; Ramírez-Bahena, Martha-Helena; Valverde, Angel; Velázquez, Encarna; Zúñiga, Doris; Velezmoro, Carmen; Peix, Alvaro

2012-09-21

199

Pedobacter luteus sp. nov., isolated from soil.  

PubMed

Two strains of Gram-staining-negative, rod-shaped bacteria that were motile by gliding, N7d-4(T) and B4a-b5, were isolated during a study of culturable bacteria in soil cultivated with potatoes. These isolates grew at 15-37 °C and at pH 6.5-7.0. The major cellular fatty acids were summed feature 3 (iso-C15?:?0 2-OH and/or C16?:?1?7c), anteiso-C15?:?0, iso-C15?:?0, iso-C17?:?0 3-OH and iso-C17?:?1?9c. The major polar lipids were phosphatidyl-N-methylethanolamine and phosphatidylethanolamine. The strains contained d-18?:?0 and d-19?:?0 sphingosines. The DNA G+C contents of strains N7d-4(T) and B4a-b5 were 48.5 and 46.9 mol% (HPLC), respectively. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains N7d-4(T) and B4a-b5 were affiliated with Pedobacter species in the family Sphingobacteriaceae. Strains N7d-4(T) and B4a-b5 shared 99.9?% sequence similarity, and the most closely related Pedobacter type strains were Pedobacter composti TR6-06(T) (96.5 and 96.7?% sequence similarity, respectively), P. oryzae N7(T) (95.4 and 95.6?%) and P. caeni LMG 22862(T) (94.0 and 94.4?%). Phenotypic data and phylogenetic inference clearly distinguished the two isolates from other Pedobacter species. Based on these data, the isolates are considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter luteus sp. nov. is proposed. The type strain is N7d-4(T) (?=?KCTC 22699(T) ?=?DSM 22385(T)). PMID:22798651

Oh, Hyun-Woo; Kim, Byung-Chun; Park, Doo-Sang; Jeong, Won-Jin; Kim, Hyangmi; Lee, Kang Hyun; Kim, Sung Uk

2012-07-13

200

Paenibacillus tianmuensis sp. nov., isolated from soil.  

PubMed

Two closely related, Gram-stain-negative, rod-shaped, spore-forming strains, B27(T) and F6-B70, were isolated from soil samples of Tianmu Mountain National Natural Reserve in Zhejiang, China. Phylogenetic analysis based on 16S rRNA gene and rpoB sequences indicated that the isolates were members of the genus Paenibacillus. Both isolates were closely related to Paenibacillus ehimensis IFO 15659(T), Paenibacillus elgii SD17(T) and Paenibacillus koreensis YC300(T) (? 95.2 % 16S rRNA gene sequence similarity). DNA-DNA relatedness between strain B27(T) and P. ehimensis DSM 11029(T), P. elgii NBRC 100335(T) and P. koreensis KCTC 2393(T) was 21.2, 28.6 and 16.8 %, respectively. The major cellular fatty acids of strains B27(T) and F6-B70 were anteiso-C(15 : 0) and iso-C(15 : 0). The cell wall contained meso-diaminopimelic acid. The two isolates differed from their closest neighbours in terms of phenotypic characteristics and cellular fatty acid profiles (such as variable for oxidase, negative for methyl red test, unable to produce acid from d-fructose and glycogen and relatively higher amounts of iso-C(15 : 0) and lower amounts of C(16 : 0) and iso-C(16 : 0)). Strains B27(T) and F6-B70 represent a novel species of the genus Paenibacillus, for which the name Paenibacillus tianmuensis sp. nov. is proposed. The type strain is B27(T) (?=?DSM 22342(T) ?=?CGMCC 1.8946(T)). PMID:20543152

Wu, Xuechang; Fang, Haihuan; Qian, Chaodong; Wen, Yanping; Shen, Xiaobo; Li, Ou; Gao, Haichun

2010-06-11

201

A mosquito predator survey in Townsville, Australia, and an assessment of Diplonychus sp. and Anisops sp. predatorial capacity against Culex annulirostris mosquito immatures.  

PubMed

A twelve-month survey for mosquito predators was conducted in Townsville, Queensland, Australia, which is located in the arid tropics. The survey revealed the presence of five predaceous insects but only Anisops sp. (backswimmers) and Diplonychus sp. were common. Predatorial capacity and factors influencing this capacity were then assessed for adult Anisops sp. and adult and nymph stages of Diplonychus sp. against Culex annulirostris mosquito immatures under laboratory conditions. Predatorial capacity bioassays showed that adult Diplonychus sp. preyed upon both larval and pupal stages of Cx. annulirostris quite successfully. Nymphs of Diplonychus sp. proved to be more successful with smaller prey immatures, and Anisops sp adults did not prey successfully on any prey pupae. Increasing the foraging area and introducing aquatic vegetation significantly reduced the predatorial capacity of Diplonychus sp. nymphs, while only vegetation and not foraging area had a significant effect on adult Diplonychus sp. predation capacity. Overall, adult Diplonychus sp. proved to be a more efficient predator than Anisops sp., and field trials are now recommended to further assess the potential of Diplonychus sp. as a biocontrol agent. PMID:17633421

Shaalan, Essam Abdel-Salam; Canyon, Deon Vahid; Muller, Reinhold; Younes, Mohamed Wagdy Faried; Abdel-Wahab, Hoda; Mansour, Abdel-Hamid

2007-06-01

202

75 FR 42380 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Contract and SP-15 Financial Day-Ahead...  

Federal Register 2010, 2011, 2012, 2013

...COMMISSION Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Contract and SP-15 Financial Day-Ahead LMP Off-Peak Contract Offered for...determination whether the SP-15 \\2\\ Financial Day-Ahead LMP Peak (``SPM'') contract...

2010-07-21

203

75 FR 42411 - Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Daily Contract; SP-15 Financial Day...  

Federal Register 2010, 2011, 2012, 2013

...COMMISSION Orders Finding That the SP-15 Financial Day-Ahead LMP Peak Daily Contract; SP-15 Financial Day-Ahead LMP Off-Peak Daily Contract; SP-15...LMP-Peak Daily Contract; NP-15 Financial Day- Ahead LMP Peak Daily Contract and...

2010-07-21

204

Pulmonary surfactant proteins SP-B and SP-C in spread monolayers at the air-water interface: I. Monolayers of pulmonary surfactant protein SP-B and phospholipids.  

PubMed Central

The effects of pulmonary surfactant protein SP-B on the properties of monolayers of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG), and a mixture of DPPC:DPPG (7:3, mol:mol) were studied using spread films at the air-water interface. The addition of SP-B to the phospholipid monolayers gave positive deviations from additivity of the mean areas in the films. At low protein concentrations (less than 45% amino acid residues which corresponds to 0.5 mol% or 10 weight% SP-B) monolayers of SP-B/DPPC, SP-B/DPPG and SP-B/(DPPC:DPPG) collapsed at surface pressures of about 70 mN.m-1, comparable to those of the lipids alone. At higher concentrations of SP-B in the protein-lipid monolayers, kink points appeared in the isotherms at about 40-45 mN.m-1, implying possible exclusion of material from the films, hence, changes in the original monolayer compositions. Calculated analyses of the monolayer compositions as a function of surface pressure indicated that nearly pure SP-B, associated with small amounts of phospholipid (2-3 lipid molecules per SP-B dimer), was lost from SP-B/DPPC, SP-B/DPPG, and SP-B/(DPPC:DPPG) films at surface pressures higher than 40-45 mN.m-1. The results are consistent with a low effectiveness of SP-B in removing saturated phospholipids, DPPC or DPPG, from the spread SP-B/phospholipid films.

Taneva, S; Keough, K M

1994-01-01

205

Oil productivity of the tropical marine diatom Thalassiosira sp.  

PubMed

To understand the potential of cultivating tropical marine diatom Thalassiosira sp. to produce biofuel, biodiesel product properties and growth characteristics of Thalassiosira sp. in three different media were investigated. After medium evaluation, significant Thalassiosira sp. cell growth was observed in both Walne and enriched seawater media, but not in plain seawater medium. The microalgae grew well in alkaline condition (pH range of 8.0-8.8). The average biomass density cultured in Walne and enriched seawater media on the 6th day was 4.36 and 2.50 g L(-1), respectively. Based on ESI-IT-MS spectra, the TAGs of algal oil were identified as POP, POO, and SOO, and the FAMEs as oleic acid methyl ester. The oil productivity of Thalassiosira sp. cultured in Walne and enriched seawater media were 150 and 290 ?L L(-1) d(-1), respectively. The density and kinematic viscosity of Thalassiosira sp. biodiesel were 0.857 g mL(-1) and 1.151 mm(2) s(-1). PMID:22264432

Nurachman, Zeily; Hartati; Anita, Syahfitri; Anward, Etsuroyya Ewidyasari; Novirani, Gestria; Mangindaan, Bill; Gandasasmita, Suryo; Syah, Yana Maolana; Panggabean, Lily Maria Goretty; Suantika, Gede

2011-12-23

206

Sp1 modifies leg-to-wing transdetermination in Drosophila.  

PubMed

During Drosophila development, the transcription factor Sp1 is necessary for proper leg growth and also to repress wing development. Here we test the role of Sp1 during imaginal disc regeneration. Ubiquitous expression of wg induces a regeneration blastema in the dorsal aspect of the leg disc. Within this outgrowth, the wing selector gene vg is activated in some cells, changing their fate to wing identity in a process known as transdetermination. In this report we demonstrate that reducing the gene copy number of Sp1 significantly increases both the frequency and the area of transdetermination in regenerating leg discs. By examining the expression of known Sp1 target genes, we also show that the proximo-distal patterning gene dachshund is downregulated dorsally, leading to a break in its normal ring-shaped expression pattern. We further report that transdetermination, as evidenced by Vg expression, is only observed when there is a broken ring of Dachshund expression. Combined, these studies establish a role for Sp1 in leg-to-wing transdetermination. PMID:23165292

Ing, Thomas; Tseng, Alexander; Sustar, Anne; Schubiger, Gerold

2012-11-17

207

Bioremediation of crude oil contamination with Acinetobacter sp. A3.  

PubMed

Acinetobacter sp. A3 is able to extensively degrade Bombay High Crude Oil (BHCO) and utilize it as the sole source of carbon. A total degradation of 70% BHCO was noted by the end of 120 h of growth of Acinetobacter sp. A3 under shake flask condition, 60% of which was due to biodegradation. In crude oil-contaminated soil (5%) amended with Acinetobacter sp. A3, there was both an increase in colony-forming units (CFU) and crude oil degradation. This is in contrast to a decrease in CFU of the indigenous microorganisms and lower degradation in unamended soil within the same 30-day period. Also, Acinetobacter sp. A3-treated soil permitted better germination of Mung beans (Phaseolus aureus) and growth as evidenced by better length and weight of the plants and chlorophyll content of its leaves, which was attributed to the reduction in phytotoxicity of the crude oil owing to its degradation. This crude oil degradative capability of Acinetobacter sp. A3 could be exploited for bioremediation purposes. PMID:9236304

Hanson, K G; Nigam, A; Kapadia, M; Desai, A J

1997-09-01

208

Chromobacterium piscinae sp. nov. and Chromobacterium pseudoviolaceum sp. nov., from environmental samples.  

PubMed

Two strains (LMG 3947 and LMG 3953) of motile, Gram-negative, violet-pigmented bacteria, respectively isolated in 1972 and before 1953 from environmental samples and previously assigned to Chromobacterium violaceum, were studied again in detail for their taxonomic position. 16S rRNA gene sequence analysis showed that both strains clustered together with the type strain of Chromobacterium violaceum (99.8 % sequence similarity for strain LMG 3953(T) and 98.9 % sequence similarity for LMG 3947(T)). recA sequence similarities were significantly lower (95-96 %), and fatty acid profiles were also different from those reported for C. violaceum and other Chromobacterium species. Both strains exhibit polar-lipid profiles consisting of the major compound phosphatidylethanolamine and moderate amounts of phosphatidylglycerol in addition to some minor lipids. The quinone systems consist of the major compound ubiquinone Q-8 and moderate amounts of Q-7. Polyamine patterns are composed of the major compounds putrescine and 2-hydroxyputrescine, moderate amounts of 1,3-diaminopropane and variable amounts of cadaverine and spermidine. The results of DNA-DNA hybridizations and physiological as well as biochemical tests allowed both genotypic and phenotypic differentiation of the two strains from described Chromobacterium species. It is evident from the genotypic and phenotypic data that both strains represent novel species in the genus Chromobacterium, for which we propose the names Chromobacterium piscinae sp. nov. (type strain LMG 3947(T) =CCM 3329(T)) and Chromobacterium pseudoviolaceum sp. nov. (type strain LMG 3953(T) =CCM 2076(T) =NCIMB [corrected] 8182(T)). PMID:19622653

Kämpfer, Peter; Busse, Hans-Jürgen; Scholz, Holger C

2009-07-21

209

Mongoliicoccus alkaliphilus sp. nov. and Litoribacter alkaliphilus sp. nov., isolated from salt pans.  

PubMed

Four bacterial strains (JC165(T), JC166(T), JC169 and JC170) were isolated from salt pan soils from a coastal region of Tamilnadu, India. They were obligately aerobic, pink- to red-pigmented, mesophilic, haloalkaliphiles having chemoorganoheterotrophic growth on various carbon sources and were catalase- and oxidase-positive. Phototrophic growth and bacteriochlorophyll a were absent in all four strains. Major carotenoids present were ?-carotene and rhodoxanthin. The main fatty acid in all strains was iso-C(15:0). The main polar lipids were phosphatidylethanolamine (PE) and phosphatidylcholine (PC) as well as a few unidentified lipids. Bacterial hopane derivatives and diplopterol (DPL) were detected in all four strains. Based on the 16S rRNA gene sequences, all four strains belong to the family Cyclobacteriaceae in the phylum Bacteroidetes. Strains JC165(T) and JC169 had a sequence similarity of 97.2% with Mongoliicoccus roseus MIM28(T), while strains JC166(T) and JC170 had a sequence similarity of 99.5% with Litoribacter ruber YIM CH208(T). Strains JC165(T)/JC169 and JC166(T)/JC170 had genomic DNA reassociation values (based on DNA-DNA hybridization) of 21 ± 2% and 23 ± 1% with M. roseus KCTC 19808(T) (?=?MIM28(T)) and L. ruber KCTC 22899(T) (?=?YIM CH208(T)), respectively, suggesting that they represented novel species. The reassociation values of >85% between strains JC165(T) and JC169, and JC166(T) and JC170 suggested they were strains of the same species. The genomic information was supported by phenotypic observations leading to the proposal of two novel species, Mongoliicoccus alkaliphilus sp. nov. (type strain, JC165(T)?=?KCTC 32210(T)?=LMG 27255(T)) and Litoribacter alkaliphilus sp. nov. (type strain, JC166(T)?=?KCTC 32217(T)?=LMG 27256(T)). PMID:23543498

Subhash, Y; Tushar, L; Sasikala, Ch; Ramana, Ch V

2013-03-29

210

Actinophytocola timorensis sp. nov. and Actinophytocola corallina sp. nov., isolated from soil.  

PubMed

Two actinomycete strains, ID05-A0653(T) and ID06-A0464(T), were isolated from soils of West Timor and Lombok island, respectively, in Indonesia. 16S rRNA gene sequence analysis clearly demonstrated that the isolates belonged to the family Pseudonocardiaceae and were closely related to the genus Actinophytocola. Strains ID05-A0653(T) and ID06-A0464(T) exhibited 98.1 and 98.2?% 16S rRNA gene sequence similarity, respectively, with Actinophytocola oryzae GMKU 367(T). The isolates grew well on ISP media and produced white aerial mycelium. Short spore chains were formed directly on the substrate mycelium. The isolates contained meso-diaminopimelic acid, arabinose and galactose as cell-wall components, MK-9(H(4)) as the sole isoprenoid quinone, iso-C(16?:?0) as the major cellular fatty acid and phosphatidylethanolamine as the diagnostic polar lipid. The DNA G+C contents of strains ID05-A0653(T) and ID06-A0464(T) were 69.7 and 71.2 mol%, respectively. On the basis of phenotypic characteristics, DNA-DNA relatedness and 16S rRNA gene sequence comparisons, strains ID05-A0653(T) and ID06-A0464(T) each represent a novel species of the genus Actinophytocola, for which the names Actinophytocola timorensis sp. nov. (type strain ID05-A0653(T) ?=?BTCC B-673(T) ?=?NBRC 105524(T)) and Actinophytocola corallina sp. nov. (type strain ID06-A0464(T) ?=?BTCC B-674(T) ?=?NBRC 105525(T)) are proposed. PMID:20495034

Otoguro, Misa; Yamamura, Hideki; Tamura, Tomohiko; Irzaldi, Rohmatussolihat; Ratnakomala, Shanti; Ridwan, Roni; Kartina, Gina; Triana, Evi; Nurkanto, Arif; Lestari, Yulin; Lisdiyanti, Puspita; Widyastuti, Yantyati; Ando, Katsuhiko

2010-05-21

211

Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea.  

PubMed

Two bacterial strains, designated GH34-4(T) and GH41-7(T), were isolated from greenhouse soil cultivated with cucumber. The bacteria were strictly aerobic, Gram-negative, rod-shaped and oxidase- and catalase-positive. 16S rRNA gene sequence analysis indicated that these strains belong to the genus Lysobacter within the Gammaproteobacteria. Strain GH34-4(T) showed highest sequence similarity to Lysobacter yangpyeongensis GH19-3(T) (97.5 %) and Lysobacter koreensis Dae16(T) (96.4 %), and strain GH41-7(T) showed highest sequence similarity to Lysobacter antibioticus DSM 2044(T) (97.5 %), Lysobacter enzymogenes DSM 2043(T) (97.5 %) and Lysobacter gummosus ATCC 29489(T) (97.4 %). Levels of DNA-DNA relatedness indicated that strains GH34-4(T) and GH41-7(T) represented species clearly different from L. yangpyeongensis, L. antibioticus, L. enzymogenes and L. gummosus. The major cellular fatty acids of strains GH34-4(T) and GH41-7(T) were iso-C(16 : 0), iso-C(15 : 0) and iso-C(17 : 1)omega9c, and the major isoprenoid quinone was Q-8. The DNA G+C contents of GH34-4(T) and GH41-7(T) were 62.5 and 66.6 mol%, respectively. On the basis of the polyphasic taxonomic data presented, it is evident that each of these strains represents a novel species of the genus Lysobacter, for which the names Lysobacter niabensis sp. nov. (type strain GH34-4(T)=KACC 11587(T)=DSM 18244(T)) and Lysobacter niastensis sp. nov. (type strain GH41-7(T)=KACC 11588(T)=DSM 18481(T)) are proposed. PMID:17329782

Weon, Hang-Yeon; Kim, Byung-Yong; Kim, Min-Kyeong; Yoo, Seung-Hee; Kwon, Soon-Wo; Go, Seung-Joo; Stackebrandt, Erko

2007-03-01

212

Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov., isolated from soil.  

PubMed

Two Gram-reaction-negative, rod-shaped, gliding, yellow-pigmented bacterial strains, designated ZLD-17(T) and ZLD-29(T), were isolated from arid soil samples collected from Xinjiang Province, north-west China, and subjected to analysis using a polyphasic taxonomic approach. Both novel strains required 1.0-2.0?% (w/v) sea salts for optimal growth. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genus Lysobacter within the class Gammaproteobacteria. Strain ZLD-17(T) showed highest 16S rRNA gene sequence similarities to Lysobacter capsici KCTC 22007(T) (96.9?%), Lysobacter spongiicola DSM 21749(T) (96.8?%) and Lysobacter koreensis KCTC 12204(T) (96.8?%), whereas strain ZLD-29(T) showed highest sequence similarities to Lysobacter niastensis DSM 18481(T) (96.0?%) and Lysobacter enzymogenes DSM 2043(T) (95.9?%). 16S rRNA gene sequence similarity between ZLD-17(T) and ZLD-29(T) was 96.1?%. The DNA G+C contents of strains ZLD-17(T) and ZLD-29(T) were 67.9 and 68.2 mol%, respectively. The major cellular fatty acids of both strains were summed feature 3 (iso-C??:? 2-OH and/or C??:??7c), iso-C??:??9c, iso-C??:?, C??:? and iso-C??:? 3-OH; their predominant isoprenoid quinone was Q-8 and their major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on their phenotypic characteristics, phylogenetic position as determined by 16S rRNA gene sequence analysis and chemotaxonomic data, strains ZLD-17(T) (?=?CCTCC AB 207174(T) ?=?KCTC 23076(T)) and ZLD-29(T) (?=?CCTCC AB 207175(T)?=?KCTC 23077(T)) represent two novel species of the genus Lysobacter, for which the names Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov. are proposed, respectively. PMID:20952549

Zhang, Lei; Bai, Juan; Wang, Yang; Wu, Gao-Lin; Dai, Jun; Fang, Cheng-Xiang

2010-10-15

213

Description of Leifsonia kafniensis sp. nov. and Leifsonia antarctica sp. nov.  

PubMed

Strains KFC-22(T) and SPC-20(T) are yellow-pigmented, Gram-positive, aerobic, non-motile, rod-shaped bacteria that were isolated from a soil sample near the Kafni glacier in the Himalayan mountain ranges in India, and from a spade core sediment sample from the Antarctic Ocean at Larsemann Hill, respectively. In both cases, the cell-wall peptidoglycan contained 2,4-diaminobutyric acid as the diamino acid, anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) were the predominant fatty acids and MK-11 was the major isoprenoid quinone in the cell membrane. On the basis of the above-mentioned characteristics, both strains can be assigned to the genus Leifsonia. The strains share 16S rRNA gene sequence similarity of 97.7 % and DNA relatedness of only 10 %, indicating that they represent different species. A blast analysis indicated that Leifsonia pindariensis PON10(T) was the closest phylogenetic neighbour of strains SPC-20(T) and KFC-22(T), showing 16S rRNA gene sequence similarities of 97.3 and 97.7 %, respectively. However, at the whole-genome level, strains KFC-22(T) and SPC-20(T) shared 42 and 11 % DNA-DNA relatedness, respectively, with L. pindariensis PON10(T). In addition, both strains exhibited several phenotypic differences with respect to L. pindariensis PON10(T). Thus, on the basis of the differences that the two strains exhibited with respect to L. pindariensis, both were identified as representing novel species of the genus Leifsonia, for which the names Leifsonia kafniensis sp. nov. (type strain KFC-22(T) =NCCB 100216(T) =LMG 24362(T)) and Leifsonia antarctica sp. nov. (type strain SPC-20(T) =NCCB 100227(T) =LMG 24541(T)) are proposed. PMID:19502314

Pindi, Pavan Kumar; Kishore, K Hara; Reddy, G S N; Shivaji, S

2009-06-01

214

Streptomyces siamensis sp. nov., and Streptomyces similanensis sp. nov., isolated from Thai soils.  

PubMed

Three actinomycete strains, KC-038(T), KC-031 and KC-106(T), were isolated from soil samples collected in the southern Thailand. The morphological and chemotaxonomic properties of strains KC-038(T), KC-031 and KC-106(T) were consistent with the characteristics of members of the genus Streptomyces, that is, the formation of aerial mycelia bearing spiral spore chains; the presence of LL-diaminopimelic acid in the cell wall, MK-9 (H6), MK-9 (H4) and MK-9 (H8) as the predominant menaquinones; and C16:0, iso-C16:0 and anteiso-C15:0 as the major cellular fatty acids. 16S rRNA gene sequence analyses indicated that strains KC-038(T) and KC-031 were highly similar (99.9%), and they were closely related to S. olivochromogenes NBRC 3178(T) (98.1%) and S. psammoticus NBRC 13971(T) (98.1%). Strain KC-106(T) was closely related to S. seoulensis NBRC 16668(T) (98.9%), S. recifensis NBRC 12813(T) (98.9%), S. chartreusis NBRC 12753(T) (98.7%) and S. griseoluteus NBRC 13375(T) (98.4%). The values of DNA-DNA relatedness between the isolates and the type strains of the related species were below 70%. On the basis of the polyphasic evidence, the isolates should be classified as two novel species, namely Streptomyces siamensis sp. nov. (type strain, KC-038(T)=NBRC 108799(T)=PCU 328(T)=TISTR 2107(T)) and Streptomyces similanensis sp. nov. (type strain, KC-106(T)=NBRC 108798(T)=PCU 329(T)=TISTR 2104(T)).The Journal of Antibiotics advance online publication, 12 June 2013; doi:10.1038/ja.2013.60. PMID:23756683

Sripreechasak, Paranee; Matsumoto, Atsuko; Suwanborirux, Khanit; Inahashi, Yuki; Shiomi, Kazuro; Tanasupawat, Somboon; Takahashi, Yoko

2013-06-12

215

Haloarcula salaria sp. nov. and Haloarcula tradensis sp. nov., isolated from salt in Thai fish sauce.  

PubMed

Two red-pigmented, strictly aerobic, pleomorphic rod-shaped and extremely halophilic archaea, designated strains HST01-2R(T) and HST03(T), were isolated from salt in a fish sauce sample from Thailand. The novel strains grew optimally at 37 °C, pH 7.0, and in the presence of 20-25?% (w/v) NaCl. The DNA G+C contents of the isolates were 61.6-62.2 mol%. Phylogenetic analysis based on a comparison of 16S rRNA gene sequences revealed that strains HST01-2R(T) and HST03(T) were placed in the radiation of species of the genus Haloarcula. The chemotaxonomic properties of the two strains, i.e. the presence of MK-8 and MK-8(H(2)) as the major menaquinone components and C(20)C(20) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a triglycosyl diether as major polar lipids, supported the assignment of the two strains to the genus Haloarcula. Nevertheless, several phenotypic features and the low DNA-DNA relatedness between the two strains and related species of the genus Haloarcula (13.4-46.9?%) enabled the strains to be distinguished from each other and from recognized species. Therefore, strains HST01-2R(T) and HST03(T) represent two novel species in the genus Haloarcula, for which the names Haloarcula salaria sp. nov. and Haloarcula tradensis sp. nov. are proposed, respectively. The type strains are HST01-2R(T) (=BCC 40029(T)=JCM 15759(T)=PCU 313(T)) and HST03(T) (=BCC 40030(T)=JCM 15760(T)=PCU 314(T)). PMID:20207809

Namwong, Sirilak; Tanasupawat, Somboon; Kudo, Takuji; Itoh, Takashi

2010-03-05

216

Bradyrhizobium pachyrhizi sp. nov. and Bradyrhizobium jicamae sp. nov., isolated from effective nodules of Pachyrhizus erosus.  

PubMed

Several strains isolated from the legume Pachyrhizus erosus were characterized on the basis of diverse genetic, phenotypic and symbiotic approaches. These novel strains formed two groups closely related to Bradyrhizobium elkanii according to their 16S rRNA gene sequences. Strains PAC48T and PAC68T, designated as the type strains of these two groups, presented 99.8 and 99.1% similarity, respectively, in their 16S rRNA gene sequences with respect to B. elkanii USDA 76T. In spite of these high similarity values, the analysis of additional phylogenetic markers such as atpD and glnII genes and the 16S-23S intergenic spacer (ITS) showed that strains PAC48T and PAC68T represented two separate novel species of the genus Bradyrhizobium with B. elkanii as their closest relative. Phenotypic differences among the novel strains isolated from Pachyrhizus and B. elkanii were found regarding the assimilation of carbon sources and antibiotic resistance. All these differences were congruent with DNA-DNA hybridization analysis which revealed 21% genetic relatedness between strains PAC48T and PAC68T and 46% and 25%, respectively, between these strains and B. elkanii LMG 6134T. The nodD and nifH genes of strains PAC48T and PAC68T were phylogenetically divergent from those of bradyrhizobia species that nodulate soybean. Soybean was not nodulated by the novel Pachyrhizus isolates. Based on the genotypic and phenotypic data obtained in this study, the new strains represent two novel species for which the names Bradyrhizobium pachyrhizi sp. nov. (type strain PAC48T=LMG 24246T=CECT 7396T) and Bradyrhizobium jicamae sp. nov. (type strain PAC68T=LMG 24556T=CECT 7395T) are proposed. PMID:19567584

Ramírez-Bahena, Martha Helena; Peix, Alvaro; Rivas, Raúl; Camacho, María; Rodríguez-Navarro, Dulce N; Mateos, Pedro F; Martínez-Molina, Eustoquio; Willems, Anne; Velázquez, Encarna

2009-06-30

217

Bifunctional properties of peroxisome proliferator-activated receptor gamma1 in KDR gene regulation mediated via interaction with both Sp1 and Sp3.  

PubMed

Vascular endothelial growth factor receptor 2 (KDR) plays a critical role in mediating a variety of vasculogenic and angiogenic processes, including diabetic retinopathy. We previously demonstrated that the promoter activity of the KDR gene in retinal capillary endothelial cells (RCECs) was regulated in part by the relative concentration of positive/negative transcription factors Sp1/Sp3. We also reported that the peroxisome proliferator-activated receptor (PPAR)gamma ligand could inhibit intraocular angiogenesis. In the present study, the role of PPARgamma1 in KDR gene regulation in RCECs was examined. PPARgamma1 protein physically interacted with both Sp1 and Sp3. Transactivation and electrophoretic mobility shift assays clearly demonstrated novel findings that PPARgamma1 increased KDR promoter activity by enhancing the interaction between Sp1, but not Sp3, and KDR promoter region without its ligand in RCECs. The ligand-binding site but not the DNA binding site of PPARgamma1 enhanced the interaction between Sp1 and KDR promoter region. Conversely, PPARgamma1 ligand 15-deoxy Delta (12,14)-prostaglandin J2 dose-dependently suppressed the binding of KDR promoter region with both Sp1 and Sp3, resulting an inhibition of KDR gene expression. In conclusion, PPARgamma1 has bifunctional properties in the regulation of KDR gene expression mediated via interaction with both Sp1 and Sp3. PMID:15111490

Sassa, Yukio; Hata, Yasuaki; Aiello, Lloyd Paul; Taniguchi, Yukio; Kohno, Kimitoshi; Ishibashi, Tatsuro

2004-05-01

218

The transcription factors Sp1, Sp3, and AP-2 are required for constitutive matrix metalloproteinase-2 gene expression in astroglioma cells.  

PubMed

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that contribute to pathological conditions associated with angiogenesis and tumor invasion. MMP-2 is highly expressed in human astroglioma cells, and contributes to the invasiveness of these cells. The human MMP-2 promoter contains potential cis-acting regulatory elements including cAMP response element-binding protein, AP-1, AP-2, PEA3, C/EBP, and Sp1. Deletion and site-directed mutagenesis analysis of the MMP-2 promoter demonstrates that the Sp1 site at -91 to -84 base pairs and the AP-2 site at -61 to -53 base pairs are critical for constitutive activity of this gene in invasive astroglioma cell lines. Electrophoretic gel shift analysis demonstrates binding of specific DNA-protein complexes to the Sp1 and AP-2 sites: Sp1 and Sp3 bind to the Sp1 site, while the AP-2 transcription factor binds the AP-2 element. Co-transfection expression experiments in Drosophilia SL2 cells lacking endogenous Sp factors demonstrate that Sp1 and Sp3 function as activators of the MMP-2 promoter and synergize for enhanced MMP-2 activation. Overexpression of AP-2 in AP-2-deficient HepG2 cells enhances MMP-2 promoter activation. These findings document the functional importance of Sp1, Sp3, and AP-2 in regulating constitutive expression of MMP-2. Delineation of MMP-2 regulation may have implications for development of new therapeutic strategies to arrest glioma invasion. PMID:10506168

Qin, H; Sun, Y; Benveniste, E N

1999-10-01

219

Purification of soyasaponin -?-galactosidase from Aspergillus sp.39  

NASA Astrophysics Data System (ADS)

In order to increase physiological activity of soyasaponin, enzyme hydrolysis of soyasaponin was studied. The enzyme which hydrolyzes soyasaponin to lower sugar soyasaponin was obtained from Aspergillus sp.39s. And it was purified by the method of biologic chromatography system. The method of SDS-polyacrylamide gel electrophoresis was used to determine the molecular weight of the enzyme produced by Aspergillus sp.39s. The molecular weight was about 50 kDa. The optimum pH and temperature of soyasaponin-?-galactosidase produced from sp.39s was 5.0 and 40°C respectively. Soyasaponin-?-galactosidase was comparatively stable in the pH range from 3.0 to 7.0 and in the temperature range from 20°C to 60°C.

Tian, Jing; Zhao, Ping; Xu, Longquan; Fei, Xu; Wang, Yi

220

Aureobasidium thailandense sp. nov. isolated from leaves and wooden surfaces.  

PubMed

Aureobasidium thailandense sp. nov. is described from cultures of material collected on leaves and wooden surfaces in Thailand and the type isolate is NRRL 58539(T). Phylogenetically it is distinct from other species of the genus Aureobasidium. Phenotypically it is distinguished by its cardinal growth temperatures, salt tolerance and production of reddish brown hyphal pigmentation in PDA cultures, but micro-morphologically it is not clearly distinguishable from Aureobasidium pullulans. Unlike A. pullulans, A. thailandense sp. nov. produces a non-pullulan extracellular polysaccharide whose characteristics are unknown. The two known isolates of A. thailandense sp. nov. possess an approx. 500 bp type I intron in the 18S rRNA gene that is present in ITS amplifications using primers ITS4 and ITS5. A. pullulans isolates uniformly lack this intron. PMID:23178722

Peterson, Stephen W; Manitchotpisit, Pennapa; Leathers, Timothy D

2012-11-23

221

Low SP1 Expression Differentially Affects Intestinal-Type Compared with Diffuse-Type Gastric Adenocarcinoma  

PubMed Central

Specificity protein 1 (SP1) is an essential transcription factor that regulates multiple cancer-related genes. Because aberrant expression of SP1 is related to cancer development and progression, we focused on SP1 expression in gastric carcinoma and its correlation with disease outcomes. Although patient survival decreased as SP1 expression increased (P<0.05) in diffuse-type gastric cancer, the lack of SP1 expression in intestinal-type gastric cancer was significantly correlated with poor survival (P<0.05). The knockdown of SP1 in a high SP1-expressing intestinal-type gastric cell line, MKN28, increased migration and invasion but decreased proliferation. Microarray data in SP1 siRNA-transfected MKN28 revealed that the genes inhibiting migration were downregulated, whereas the genes negatively facilitating proliferation were increased. However, both migration and invasion were decreased by forced SP1 expression in a low SP1-expressing intestinal-type gastric cell line, AGS. Unlike the intestinal-type, in a high SP1-expressing diffuse-type gastric cell line, SNU484, migration and invasion were decreased by SP1 siRNA. In contrast to previous studies that did not identify differences between the 2 histological types, our results reveal that low expression of SP1 is involved in cancer progression and metastasis and differentially affects intestinal-type compared with diffuse-type gastric adenocarcinoma.

Oh, Ensel; Erkin, Ozgur Cem; Jung, Hun Soon; Cho, Mi-Hyun; Kwon, Mi Jeong; Chae, Seoung Wan; Kim, Seok-Hyung; Wang, Li-Hui; Park, Min-Jeong; Lee, Su-Yeon; Yang, Ho Bin; Jia, Lina; Choi, Yoon-La; Shin, Young Kee

2013-01-01

222

Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization  

PubMed Central

Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development. The molecular mechanisms of ameloblast differentiation (amelogenesis) are currently not well understood. SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner. Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis. However, it remains unclear how Sp6 controls amelogenesis. In this study, we used SP6 high producer cells to identify SP6 target genes. Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway. We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis. Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis.

Utami, Trianna W.; Miyoshi, Keiko; Hagita, Hiroko; Yanuaryska, Ryna Dwi; Horiguchi, Taigo; Noma, Takafumi

2011-01-01

223

Transcription factor Sp3 is silenced through SUMO modification by PIAS1  

PubMed Central

Sp3 is a ubiquitous transcription factor closely related to Sp1. Here we show that Sp3 is a target for SUMO modification in vivo and in vitro. SUMO modification of Sp3 occurs at a single lysine located between the second glutamine-rich activation domain and the DNA-binding domain. Mutational analyses identified the sequence IKXE as essential for SUMO conjugation to Sp3. We identified the protein inhibitor of activated STAT1 (PIAS1) as an interaction partner of Sp3 and Ubc9. Moreover, PIAS1 strongly stimulated SUMO conjugation to Sp3, thus acting as an E3 ligase for SUMO conjugation to Sp3. All mutations that prevented SUMO modification in vitro strongly enhanced the transcriptional activity of Sp3, showing that SUMO modification silences Sp3 activity. SUMO-modified Sp3 bound to DNA with similar specificity and affinity as unmodified Sp3. However, DNA-bound Sp3 did not act as a substrate for SUMO modification.

Sapetschnig, Alexandra; Rischitor, Grigore; Braun, Harald; Doll, Andreas; Schergaut, Marion; Melchior, Frauke; Suske, Guntram

2002-01-01

224

Sp100A promotes chromatin decondensation at a cytomegalovirus-promoter-regulated transcription site.  

PubMed

Promyelocytic leukemia nuclear bodies (PML-NBs)/nuclear domain 10s (ND10s) are nuclear structures that contain many transcriptional and chromatin regulatory factors. One of these, Sp100, is expressed from a single-copy gene and spliced into four isoforms (A, B, C, and HMG), which differentially regulate transcription. Here we evaluate Sp100 function in single cells using an inducible cytomegalovirus-promoter-regulated transgene, visualized as a chromatinized transcription site. Sp100A is the isoform most strongly recruited to the transgene array, and it significantly increases chromatin decondensation. However, Sp100A cannot overcome Daxx- and ?-thalassemia mental retardation, X-linked (ATRX)-mediated transcriptional repression, which indicates that PML-NB/ND10 factors function within a regulatory hierarchy. Sp100A increases and Sp100B, which contains a SAND domain, decreases acetyl-lysine regulatory factor levels at activated sites, suggesting that Sp100 isoforms differentially regulate transcription by modulating lysine acetylation. In contrast to Daxx, ATRX, and PML, Sp100 is recruited to activated arrays in cells expressing the herpes simplex virus type 1 E3 ubiquitin ligase, ICP0, which degrades all Sp100 isoforms except unsumoylated Sp100A. The recruitment Sp100A(K297R), which cannot be sumoylated, further suggests that sumoylation plays an important role in regulating Sp100 isoform levels at transcription sites. This study provides insight into the ways in which viruses may modulate Sp100 to promote their replication cycles. PMID:23485562

Newhart, Alyshia; Negorev, Dmitri G; Rafalska-Metcalf, Ilona U; Yang, Tian; Maul, Gerd G; Janicki, Susan M

2013-03-13

225

Genome sequence and description of Aeromicrobium massiliense sp. nov.  

PubMed

Aeromicrobium massiliense strain JC14(T)sp. nov. is the type strain of Aeromicrobium massiliense sp. nov., a new species within the genus Aeromicrobium. This strain, whose genome is described here, was isolated from the fecal microbiota of an asymptomatic patient. Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes. PMID:23408663

Ramasamy, Dhamodharan; Kokcha, Sahare; Lagier, Jean-Christophe; Nguyen, Thi-Thien; Raoult, Didier; Fournier, Pierre-Edouard

2012-11-15

226

Fuels irradiation testing for the SP-100 program  

NASA Astrophysics Data System (ADS)

An SP-100 fuel pin irradiation testing program is well on the way to providing data for performance correlations and demonstrating the lifetime and safety of the fuel system of the compact lithium-cooled reactor. Key SP-100 fuel performance issues addressed are the need for low fuel swelling and low fission gas release to minimize cladding strain, and the need for barrier integrity to prevent fuel/cladding chemical interaction. This paper provides a description of the irradiation test program that addresses these key issues and summarizes recent results of posttest examinations including data obtained at 6 atom percent goal burnup.

Makenas, Bruce J.; Hales, Janell W.; Ward, Alva L.

227

Naphthomycins L-N, ansamycin antibiotics from Streptomyces sp. CS.  

PubMed

Previous analyses of the naphthomycin biosynthetic gene cluster and a comparison with known naphthomycin-type products from Streptomyces sp. CS have suggested that new products can be found from this strain. In this study, screening by LC-MS of Streptomyces sp. CS products formed under different culture conditions revealed several unknown peaks in the product spectra of extracts derived from oatmeal medium cultures. Three new naphthomycins, naphthomycins L (1), M (2), and N (3), and the known naphthomycins A (4), E (5), and D (6) were obtained. The structures were elucidated using spectroscopic data from 1D and 2D NMR and HRESIMS experiments. PMID:22742732

Yang, Yin-He; Fu, Xiao-Li; Li, Liang-Qun; Zeng, Ying; Li, Cheng-Yun; He, Yi-Neng; Zhao, Pei-Ji

2012-06-28

228

Rhodium-catalyzed cascade oxidative annulation leading to substituted naphtho[1,8-bc]pyrans by sequential cleavage of C(sp2)-H/C(sp3)-H and C(sp2)-H/O-H bonds.  

PubMed

The cascade oxidative annulation reactions of benzoylacetonitrile with internal alkynes proceed efficiently in the presence of a rhodium catalyst and a copper oxidant to give substituted naphtho[1,8-bc]pyrans by sequential cleavage of C(sp(2))-H/C(sp(3))-H and C(sp(2))-H/O-H bonds. These cascade reactions are highly regioselective with unsymmetrical alkynes. Experiments reveal that the first-step reaction proceeds by sequential cleavage of C(sp(2))-H/C(sp(3))-H bonds and annulation with alkynes, leading to 1-naphthols as the intermediate products. Subsequently, 1-naphthols react with alkynes by cleavage of C(sp(2))-H/O-H bonds, affording the 1:2 coupling products. Moreover, some of the naphtho[1,8-bc]pyran products exhibit intense fluorescence in the solid state. PMID:22989331

Tan, Xing; Liu, Bingxian; Li, Xiangyu; Li, Bin; Xu, Shansheng; Song, Haibin; Wang, Baiquan

2012-09-25

229

76 FR 73483 - Airworthiness Directives; Gulfstream Aerospace Corporation Model GV and GV-SP Airplanes  

Federal Register 2010, 2011, 2012, 2013

...Airworthiness Directives; Gulfstream Aerospace Corporation Model GV and GV-SP Airplanes...directive (AD) for certain Gulfstream Aerospace Corporation Model GV and GV-SP airplanes...identified in this AD, contact Gulfstream Aerospace Corporation, Technical...

2011-11-29

230

SP7 Inhibits Osteoblast Differentiation at a Late Stage in Mice  

PubMed Central

RUNX2 and SP7 are essential transcription factors for osteoblast differentiation at an early stage. Although RUNX2 inhibits osteoblast differentiation at a late stage, the function of SP7 at the late stage of osteoblast differentiation is not fully elucidated. Thus, we pursued the function of SP7 in osteoblast differentiation. RUNX2 induced Sp7 expression in Runx2?/? calvarial cells. Adenoviral transfer of sh-Sp7 into primary osteoblasts reduced the expression of Alpl, Col1a1, and Bglap2 and mineralization, whereas that of Sp7 reduced Bglap2 expression and mineralization at a late stage of osteoblast differentiation. Sp7 transgenic mice under the control of 2.3 kb Col1a1 promoter showed osteopenia and woven-bone like structure in the cortical bone, which was thin and less mineralized, in a dose-dependent manner. Further, the number of processes in the osteoblasts and osteocytes was reduced. Although the osteoblast density was increased, the bone formation was reduced. The frequency of BrdU incorporation was increased in the osteoblastic cells, while the expression of Col1a1, Spp1, Ibsp, and Bglap2 was reduced. Further, the osteopenia in Sp7 or Runx2 transgenic mice was worsened in Sp7/Runx2 double transgenic mice and the expression of Col1a1 and Bglap2 was reduced. The expression of Sp7 and Runx2 was not increased in Runx2 and Sp7 transgenic mice, respectively. The expression of endogenous Sp7 was increased in Sp7 transgenic mice and Sp7-transduced cells; the introduction of Sp7 activated and sh-Sp7 inhibited Sp7 promoter; and ChIP assay showed the binding of endogenous SP7 in the proximal region of Sp7 promoter. These findings suggest that SP7 and RUNX2 inhibit osteoblast differentiation at a late stage in a manner independent of RUNX2 and SP7, respectively, and SP7 positively regulates its own promoter.

Maruyama, Zenjiro; Miyazaki, Toshihiro; Kawasaki, Keishi; Furuichi, Tatsuya; Fukuyama, Ryo; Mori, Masako; Yamana, Kei; Nakamura, Kouhei; Liu, Wenguang; Toyosawa, Satoru; Moriishi, Takeshi; Kawaguchi, Hiroshi; Takada, Kenji; Komori, Toshihisa

2012-01-01

231

Mice deficient in surfactant protein A (SP-A) and SP-D or in TLR2 manifest delayed parturition and decreased expression of inflammatory and contractile genes.  

PubMed

Previously we obtained compelling evidence that the fetus provides a critical signal for the initiation of term labor through developmental induction of surfactant protein (SP)-A expression by the fetal lung and secretion into amniotic fluid (AF). We proposed that interactions of AF macrophage (M?) Toll-like receptors (TLRs) with SP-A, at term, or bacterial components, at preterm, result in their activation and migration to the pregnant uterus. Herein the timing of labor in wild-type (WT) C57BL/6 mice was compared with mice homozygous null for TLR2, SP-A, SP-D, or doubly deficient in SP-A and SP-D. Interestingly, TLR2(-/-) females manifested a significant (P < 0.001) delay in timing of labor compared with WT as well as reduced expression of the myometrial contraction-associated protein (CAP) gene, connexin-43, and M? marker, F4/80, at 18.5 d postcoitum (dpc). Whereas in first pregnancies, SP-A(-/-), SP-D(-/-), and SP-A/D(-/-) females delivered at term (?19.5 dpc), in second pregnancies, parturition was delayed by approximately 12 h in SP-A(-/-) (P = 0.07) and in SP-A/D(-/-) (P <0.001) females. Myometrium of SP-A/D(-/-) females expressed significantly lower levels of IL-1?, IL-6, and CAP genes, connexin-43, and oxytocin receptor at 18.5 dpc compared with WT. F4/80(+) AF M?s from TLR2(-/-) and SP-A/D(-/-) mice expressed significantly lower levels of both proinflammatory and antiinflammatory activation markers (e.g. IL-1?, IL-6, ARG1, YM1) compared with gestation-matched WT AF M?s. These novel findings suggest that the pulmonary collectins acting via TLR2 serve a modulatory role in the timing of labor; their relative impact may be dependent on parity. PMID:23183169

Montalbano, Alina P; Hawgood, Samuel; Mendelson, Carole R

2012-11-26

232

Sphaerochaeta globosa gen. nov., sp. nov. and Sphaerochaeta pleomorpha sp. nov., free-living, spherical spirochaetes.  

PubMed

Free-living bacteria with spherical cells 0.5-2.5 µm in diameter were isolated from freshwater sediment. 16S rRNA gene sequence analysis placed the new isolates within the phylum Spirochaetes ('spirochaetes'). The isolates never displayed a helical morphology or motility. Growth occurred in the presence of 100 mg ampicillin l(-1) in complex and defined mineral salts medium amended with vitamins, yeast extract and monosaccharides, disaccharides or soluble starch as fermentable substrates. Two distinct isolates, designated Buddy(T) and Grapes(T), exhibited doubling times of 21±2 and 15±1 h in glucose-amended medium and grew at 15-37 and 15-30 °C. Optimum growth was observed between 25 and 30 °C and pH 6.5-7.5, with no growth below pH 5 or above pH 10. Hexose and pentose fermentation yielded ethanol, acetate and formate as major end products. Growth was strictly fermentative and anaerobic, but the isolates tolerated brief oxygen exposure. Nitrate, sulfate, thiosulfate and carbon dioxide were not used as electron acceptors, but soluble Fe(III) was reduced to Fe(II) in glucose-amended medium. The DNA G+C base contents of isolates Buddy(T) and Grapes(T) were 45.5-46.4 and 47.0-49.2 mol%, respectively. Phospholipid fatty acid (PLFA) profiles contained large proportions of C(14:0) and C(16:0) straight-chain saturated fatty acids; C(16:1)?7c and C(16:1)?9c dominated the mono-unsaturated PLFAs in isolate Grapes(T), whereas isolate Buddy(T) also possessed C(18:1)?5c, C(18:1)?7c and C(18:1)?9c fatty acids. Branched monoenoic acids accounted for up to 12.4 and 30% of the total PLFA in isolates Grapes(T) and Buddy(T), respectively. Based on their unique morphological features and the phylogenetic distance from their closest relatives, we propose the new genus, Sphaerochaeta gen. nov., to accommodate the new isolates within the novel species Sphaerochaeta globosa sp. nov. (type strain Buddy(T) =DSM 22777(T) =ATCC BAA-1886(T)) and Sphaerochaeta pleomorpha sp. nov. (type strain Grapes(T) =DSM 22778(T) =ATCC BAA-1885(T)). Sphaerochaeta globosa is the type species of the genus. PMID:21398503

Ritalahti, Kirsti M; Justicia-Leon, Shandra D; Cusick, Kathleen D; Ramos-Hernandez, Natalia; Rubin, Michael; Dornbush, Jessica; Löffler, Frank E

2011-03-11

233

Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae).  

PubMed

Two Gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains FFA1(T) and FFA3(T), and belonging to the class Gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (Diptera: Sarcophagidae). Phylogenetic analysis of 16S rRNA gene sequence data placed these two strains within the genus Ignatzschineria with similarities of 98.6 % (FFA1(T)) and 99.35 % (FFA3(T)) to Ignatzschineria larvae L1/68(T). The level of gene sequence similarity between strains FFA1(T) and FFA3(T) was 99 %, 97.15 % and 78.1 % based on the 16S rRNA, 23S rRNA and gyrB gene sequences, respectively. Strains FFA1(T) and FFA3(T) shared 24 % DNA-DNA relatedness. DNA-DNA hybridization revealed a very low level of relatedness between the novel strains (22?% for strain FFA1(T) and 44 % for strain FFA3(T)) and I. larvae L1/68(T) genomic DNA. The respiratory quinone was Q-8 in both novel strains. The DNA G+C contents were 41.1 mol% and 40.1 mol% for strains FFA1(T) and FFA3(T), respectively. The cell membrane of both strains consisted of phosphatidylglycerol, phosphatidylethanolamine, phospholipids and aminophospholipid. The major fatty acids for both strains were C(16 : 0), summed feature 8 (C(18 : 1)?7c and/or C(18 : 1)?6c), CyC(19 : 0)?8c and C(14 : 0). The results of DNA-DNA hybridization between the two new strains and I. larvae L1/68(T), in combination with phylogenetic, chemotaxonomic, biochemical and electron microscopic data, demonstrated that strains FFA1(T) and FFA3(T) represented two novel species of the genus Ignatzschineria for which the names Ignatzschineria indica sp. nov. (type strain FFA1(T)?=?DSM 22309(T)?=?KCTC 22643(T)?=?NCIM 5325(T)) and Ignatzschineria ureiclastica sp. nov. (type strain FFA3(T)?=?DSM 22310(T)?=?KCTC 22644(T)?=?NCIM 5326(T)) are proposed. PMID:20584814

Gupta, Arvind Kumar; Dharne, Mahesh Shantappa; Rangrez, Ashraf Yusuf; Verma, Pankaj; Ghate, Hemant V; Rohde, Manfred; Patole, Milind Shivaji; Shouche, Yogesh Shreepad

2010-06-28

234

Sphingobacterium cladoniae sp. nov., isolated from lichen, Cladonia sp., and emended description of Sphingobacterium siyangense.  

PubMed

A strictly aerobic, Gram-stain-negative bacterium, designated strain No.6(T), was isolated from a lichen (Cladonia sp.) collected in Geogeum Island, Korea, and its taxonomic status was established by a polyphasic study. Cells of strain No.6(T) were non-motile, catalase- and oxidase-positive, non-spore-forming rods. Growth was observed at 15-35 °C (optimum, 25-30 °C), at pH 5.0-10.0 (optimum, pH 6.0-8.0) and with 0-3?% NaCl (optimum, 0-2?%). The predominant cellular fatty acids were summed feature 3 (comprising iso-C(15?:?0) 2-OH and/or C(16?:?1)?7c, 41.5?%), iso-C(15?:?0) (26.7?%) and C(16?:?0) (9.6?%), and menaquinone MK-7 was the only respiratory quinone. The G+C content of the genomic DNA of strain No.6(T) was 36.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain No.6(T) fell within the evolutionary group encompassed by the genus Sphingobacterium. Levels of 16S rRNA gene sequence similarity between the novel strain and the type strains of recognized Sphingobacterium species ranged from 92.1 to 99.1?%, the highest values being with Sphingobacterium siyangense SY1(T) (99.1?%) and Sphingobacterium multivorum IAM 14316(T) (98.5?%). DNA-DNA relatedness between strain No.6(T) and these two type strains were 32.0 and 5.7?%, respectively. The polar lipids found in strain No.6(T) were phosphatidylethanolamine, two unidentified phospholipids, three unidentified aminophospholipids, one glycolipid and four unidentified lipids. One unidentified sphingolipid was also found. On the basis of phenotypic and genotypic data, strain No.6(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium cladoniae sp. nov. is proposed. The type strain is No.6(T) (?=?KCTC 22613(T)?=?JCM 16113(T)). An emended description of Sphingobacterium siyangense is also proposed. PMID:22561594

Lee, Dong-Heon; Hur, Jae Seoun; Kahng, Hyung-Yeel

2012-05-04

235

Effect of SP-B Peptides on the Uptake of Liposomes by Alveolar Cells  

Microsoft Academic Search

Background: Exogenous surfactant has been accepted worldwide as a therapy of RDS in premature and term infants. Exogenous surfactant is usually derived from lung extracts containing phospholipids and the surfactant proteins SP-B and SP-C. Synthetic peptides of SP-B and SP-C are being tested with the aim to develop a completely synthetic surfactant preparation. Nevertheless, the effects of these peptides on

D. L. Poelma; F. J. Walther; A. J. Waring; J. J. Haitsma; L. J. Zimmermann; B. Lachmann; J. F. van Iwaarden

2007-01-01

236

The lithosphere-asthenosphere boundary beneath Australia imaged by Sp phases  

Microsoft Academic Search

The goal of this study was to image the lithosphere-asthenosphere boundary (LAB) beneath Australia. Sp and Ps receiver functions for nineteen stations in Australia were calculated using waveforms from pre-critical paths for events at epicentral distances of 35°-80° for Ps and 55°-75° for Sp, with depths of less than 300 km for Sp. Mantle discontinuities were interpreted from Sp receiver

H. A. Ford; K. M. Fischer; D. L. Abt; L. T. Elkins-Tanton; C. A. Rychert

2009-01-01

237

Factors affecting ?-linolenic acid content in fermented glutinous rice brewed by Rhizopus sp  

Microsoft Academic Search

Fermented glutinous rice is a popular oriental food brewed by Rhizopus sp. in China. Although Rhizopus sp. Q303 can produce ?-linolenic acid (GLA), GLA content in fermented glutinous rice brewed by Xiaoqu of Rhizopus sp. Q303 greatly varied in terms of brewing temperature, from zero to 1.4±1.1% in total fatty acids, and less than that in mycelia of Rhizopus sp.

Liu Gui-you; S. Yuan Sheng; Dai Chuan-chao

2004-01-01

238

Research in codec optimization and the application of SP-frame in H.264\\/AVC  

Microsoft Academic Search

The new SP-frame, which can replace intra-frame, is used for bitstream switching. In this paper, first, the SP-frame's main applications are introduced; second, by analyzing the original SP-frame coding process, the new scheme is proposed, which allows drift-free bitstream switching; finally, a lot of simulation results are used to compare SP-frame coding efficiency with other frames. The experimental results indicate,

Yifeng Liu; Chuangbai Xiao; Ligang Zhen

2009-01-01

239

Metschnikowia chrysoperlae sp. nov., Candida picachoensis sp. nov. and Candida pimensis sp. nov., isolated from the green lacewings Chrysoperla comanche and Chrysoperla carnea (Neuroptera: Chrysopidae).  

PubMed

Fourteen yeast isolates comprising three taxa were cultured from digestive tracts of adult Chrysoperla species (Neuroptera: Chrysopidae) and their eggs. The yeast taxa were distinguished based on an estimated molecular phylogeny, DNA sequences and traditional taxonomic criteria. The new yeasts are closely related to Metschnikowia pulcherrima but are sufficiently distinguished by sequence comparison of rRNA gene sequences to consider them as novel species. Here, three novel species are described and their relationships with other taxa in the Saccharomycetes are discussed. Metschnikowia chrysoperlae sp. nov. (type strain, NRRL Y-27615T = CBS 9803T) produced needle-shaped ascospores and was the only teleomorph found. Large numbers of chlamydospores similar to those observed in M. pulcherrima were also produced. The other two novel species are asexual yeasts, Candida picachoensis sp. nov. (type strain, NRRL Y-27607T = CBS 9804T) and Candida pimensis sp. nov. (type strain, NRRL Y-27619T = CBS 9805T), sister taxa of M. chrysoperlae and M. pulcherrima. A specialized relationship between yeasts and lacewing hosts may exist, because the yeasts were isolated consistently from lacewings only. Although M. chrysoperlae was isolated from eggs and adult lacewings, suggesting the possibility of vertical transmission, no yeast was isolated from larvae. PMID:15388758

Suh, Sung-Oui; Gibson, Cara M; Blackwell, Meredith

2004-09-01

240

Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov.  

PubMed

Strains of fluorescent pseudomonads, isolated from the phyllosphere of grasses, were analysed by a polyphasic approach in order to clarify their interspecific position. Classification on the basis of ribotyping revealed six genotypes; four of these, which could be differentiated clearly from each other and from Pseudomonas species with validly published names on the basis of phenotypic features, were chosen for detailed phylogenetic analysis. DNA-DNA hybridization studies among representative strains of the four genotypes and closely related Pseudomonas species, determined by comparison of 16S rDNA sequences, showed that three of the studied ribotypes represented novel species. Two of them were related to mainly saprophytic fluorescent pseudomonads and could be easily distinguished by a negative arginine dihydrolase reaction. One ribotype, also characterized by a negative arginine dihydrolase reaction, was closely related to potentially plant-pathogenic fluorescent pseudomonads and differed in certain phenotypic features from its phylogenetic neighbours. As a consequence of the phenotypic and phylogenetic analyses, Pseudomonas trivialis sp. nov. (type strain: P 513/19(T)=DSM 14937(T)=LMG 21464(T)), Pseudomonas poae sp. nov. (type strain: P 527/13(T)=DSM 14936(T)=LMG 21465(T)) and Pseudomonas congelans sp. nov. (type strain: P 538/23(T)=DSM 14939(T)=LMG 21466(T)) are proposed. PMID:13130034

Behrendt, Undine; Ulrich, Andreas; Schumann, Peter

2003-09-01

241

Increased degradation of straw by Pleurotus ostreatus sp. ‘florida’  

Microsoft Academic Search

The growth of Pleurotus ostreatus sp. ‘florida’ on cotton and wheat straw supplemented with cotton straw water extract was examined. It was found that degradation of cotton straw was superior to that of wheat straw and that addition of water extract of cotton straw increased the degradation of wheat straw. The growth enhancing effect of this water extract could be

M. W. Platt; Y. Hadar; Y. Henis; I. Chet

1983-01-01

242

Recall of m2000sp Instrument-Abbott Molecular  

Center for Biologics Evaluation and Research (CBER)

... Solid Waste Chute (DiTi Slide) for use with the Abbott m2000sp (List 9K14-01 and 9K14-02) Automated Sample Preparation System. ... More results from www.fda.gov/biologicsbloodvaccines/safetyavailability/recalls

243

Coprosma talbrockiei sp. nov. and allied creeping species (Rubiaceae)  

Microsoft Academic Search

Coprosma talbrockiei Moore et Mason sp. nov. from north-west Nelson, New Zealand, is placed in the same Section as the Tasmanian C. moorei Rodway. In several respects these two species resemble C. atropurpurea (Ckn. et Allan) L. B. Moore stat. nov. which is shown to be specifically distinct from C. petriei Cheesem. A dichotomous key includes also the fourth New

L. B. Moore; R. Mason

1974-01-01

244

No associations of human pulmonary tuberculosis with Sp110 variants  

PubMed Central

Background After a recent report on the role of the Ipr1 gene in mediating innate immunity in a mouse model of Mycobacterium tuberculosis infection, the human Ipr1 homologue, Sp110, was considered a promising candidate for an association study in human tuberculosis. Methods In a sample of >1000 sputum positive, HIV negative West African patients with pulmonary tuberculosis and >1000 exposed, apparently healthy controls, we have genotyped 21 Sp110 gene variants that were either available from public databases, including HapMap data, or identified by DNA re?sequencing. Results No significant differences in the frequencies of any of the 21 variants were observed between patients and controls. This applied also for HapMap tagging variants and the corresponding haplotypes, when including sliding window analyses with three adjacent variants, and when stratifying controls for positivity and negativity according to the results of intradermal tuberculin (purified protein derivative, PPD) skin tests. DNA re?sequencing revealed 13 novel Sp110 variants in the 5??UTR, exons, and adjacent intronic regions. Conclusions Based on the results obtained in this case?control study, the hypothesis that Sp110 variants and haplotypes might be associated with distinct phenotypes of human M tuberculosis infection is doubtful.

Thye, T; Browne, E N; Chinbuah, M A; Gyapong, J; Osei, I; Owusu-Dabo, E; Niemann, S; Rusch-Gerdes, S; Horstmann, R D; Meyer, C G

2006-01-01

245

Steroid biotransformation by different strains of Micrococcus sp.  

PubMed

A strain of Micrococcus sp. was isolated for its capability of side chain degradation of cholesterol. This strain was characterized and identified as Micrococcus roseus. It was found to be the best strain for the production of androsta-1,4-diene-3,17-dione and androst-4-ene-3,17-dione compared with other Micrococcus strains. PMID:11501468

Dogra, N; Qazi, G N

2001-01-01

246

'Brugia timori' Sp. N. (Nematoda: Filarioidea) from Flores Island, Indonesia.  

National Technical Information Service (NTIS)

Brugia timori sp. n. from experimentally-infected Mongolian jirds (Meriones unguiculatus) is described. The adult male differs from other Brugia species, except Brugia malayi, in having a spicular ratio of 3 : 1; it differs from B. malayi in having greate...

F. Partono Purnomo D. T. Dennis S. Atmosoedjono Oemijati

1976-01-01

247

Feather degradation by Bacillus sp. FK 46 in submerged cultivation  

Microsoft Academic Search

Cultivation conditions affecting feather degradation by Bacillus sp. FK 46 were investigated. The results showed that feather was almost completely degraded under the following conditions: 1% whole chicken feather as a substrate at the initial medium pH of 9 with 5% bacterial inoculum, at a temperature of 37 °C and a shaking speed of 250 rev\\/min. Glucose, methanol, Tween 80

W Suntornsuk; L Suntornsuk

2003-01-01

248

Hexaprenoid hydroquinones from the sponge Haliclona (aka Adocia) sp.  

PubMed

Three new hexaprenoid hydroquinones, adociaquinol (1), adociasulfate 11 (2), and adociasulfate 12 (3), together with the known adociasulfates 2, 4, and 6, were isolated from the marine sponge Haliclona (aka Adocia) sp. The structures of these compounds were elucidated by interpretation of spectroscopic data. PMID:16872132

West, Lyndon M; Faulkner, D John

2006-07-01

249

3-Alkylpyridinium alkaloids from the Pacific sponge Haliclona sp.  

PubMed

The analysis of the polar extracts of the Pacific sponge Haliclona sp. yielded new dimeric (1), trimeric (2), and polymeric 3-alkylpyridinium alkaloids. Their isolation and structural elucidation, based on NMR and MS data, are discussed in detail, along with their cytotoxic activity. PMID:19133758

Casapullo, Agostino; Pinto, Oscar Cobar; Marzocco, Stefania; Autore, Giuseppina; Riccio, Raffaele

2009-02-27

250

A new antifouling hexapeptide from a Palauan sponge, Haliclona sp.  

PubMed

Bioassay-guided fractionation of an extract of the sponge Haliclona sp. provided a known hexapeptide, waiakeamide (1), and a new sulfone derivative (2). The structures of hexapeptides 1 and 2 were elucidated by extensive NMR analyses and the advanced Marfey's method with LC/MS. These compounds showed repellent activity against the blue mussel, Mytilus edulis galloprovincialis. PMID:12762818

Sera, Yutaka; Adachi, Kyoko; Fujii, Kiyonaga; Shizuri, Yoshikazu

2003-05-01

251

Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium.  

PubMed

Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various chain lengths and degrees of saturation at the C-3 position. We have isolated a bacterium, RB-44, from a site which was formally a landfill dumping ground. Using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis, this isolate was identified as a Pandoraea sp.which was then screened for AHL production using biosensors which indicated its quorum sensing properties. To identify the AHL profile of Pandoraea sp. RB-44, we used high resolution tandem mass spectrometry confirming that this isolate produced N-octanoylhomoserine lactone (C8-HSL). To the best of our knowledge, this is the first report that showed quorum sensing activity exhibited by Pandoraea sp. Our data add Pandoraea sp. to the growing number of bacteria that possess QS systems. PMID:24145919

Han-Jen, Robson Ee; Wai-Fong, Yin; Kok-Gan, Chan

2013-10-18

252

[Comparison of different transformation methods for Monascus sp].  

PubMed

In order to facilitate the producer of polyketide pathway, four different transformation methods were tested and compared in an attempt to develop the genetic transformation system of Monascus sp. Using vector pBC-Hygro, the fungus was transformed to be hygromycin B-resistant, by conventional transformation as well as electroporation based on protoplast, electroporation based on germinated conidia, and restriction enzyme-mediated integration (REMI). Electroporation based on germinated conidia was found to be inappropriate for transforming Monascus sp. due to a low transformation frequency. The conventional transformation and electroporation technique based on protoplasts were thought not to be fit for transforming Monascus sp., due to a low stability of transformants though they yielded up to 135 transformants and 125 transformants per microgrammol/Lol/Le DNA, respectively. Transformant number was increased by 20-fold by REMI (2,500 transformants per microgrammol/Lol/Le DNA) and 70%-75% of them were stable. REMI technique would be very beneficial to the establishment of the genetic transformation system of Monascus sp. PMID:16606603

Zhou, Li-Hong; Wang, Zheng-Xiang; Zhuge, Jian

2006-04-01

253

A new cembranoid from the Hainan soft coral Sinularia sp.  

PubMed

A new cembrane-type diterpene, diepoxycembrene A (1), has been isolated from the Hainan soft coral Sinularia sp. Its structure was elucidated on the basis of detailed analysis of its spectroscopic data, and by comparison of its NMR spectral data with those of the related model compounds. PMID:19031249

Qin, Song; Huang, Hui; Guo, Yue-Wei

254

Fusarium oxysporum f. sp. vasinfectum race 4 in California  

Technology Transfer Automated Retrieval System (TEKTRAN)

A brief review of research on Fusarium oxysporum Schlechtend.:Fr. f. sp. vasinfectum (Atk.) W.C. Snyder & H.N. Hans. race 4 in California is presented. Fusarium wilt has recently emerged as the dominant disease concern for cotton (Gossypium hirsutum L., G. barbadense L.) growers in California. An es...

255

MAPPING FUSARIUM SOLANI F. SP. GLYCINES RESISTANT LOCI IN SOYBEAN  

Technology Transfer Automated Retrieval System (TEKTRAN)

Sudden death syndrome (SDS) of soybean (Glycine max L. Merr.) is an important soybean disease caused by the soil-borne fungus Fusarium solani f. sp. glycines. Yield loss from SDS in severely affected areas can range from slight to nearly 100 percent. The most viable option for SDS disease management...

256

Nutritional evaluation of Cyanobacterium (Nostoc sp.) extract in Rhizobium cultures  

Microsoft Academic Search

Extracts from Nostoc sp. biomass (CE) were compared with yeast extract (YE) in the formulation of media for cultivation of Rhizobium etli and Bradyrhizobium japonicum. Growth depended on the rhizobium strain and also on the type and concentration of the extract. The growth kinetics of R. etli were substantially influenced by the addition of CE an YE at concentrations between

P. G. Silva; D. M. Gonza´lez; E. G. Aguilar; H. J. Silva

1998-01-01

257

Spathaspora brasiliensis sp. nov., Spathaspora suhii sp. nov., Spathaspora roraimanensis sp. nov. and Spathaspora xylofermentans sp. nov., four novel (D)-xylose-fermenting yeast species from Brazilian Amazonian forest.  

PubMed

Four new D-xylose fermenting yeast species of the clade Spathaspora were recovered from rotting-wood samples in a region of Amazonian forest, Northern Brazil. Three species produced unconjugated asci with a single elongated ascospore with curved ends. These species are described as Spathaspora brasiliensis, Spathaspora suhii and Spathaspora roraimanensis. Two isolates of an asexually reproducing species belonging to the Spathaspora clade were also obtained and they are described as Spathaspora xylofermentans. All these species are able to ferment D-xylose during aerobic batch growth in rich YP (1 % yeast extract, 2 % peptone and 2 % D-xylose) medium, albeit with differing efficiencies. The type strains are Spathaspora brasiliensis sp. nov UFMG-HMD19.3 (=CBMAI 1425=CBS 12679), Spathaspora suhii sp. nov. UFMG-XMD16.2 (=CBMAI 1426=CBS 12680), Spathaspora roraimanensis sp. nov. UFMG-XMD23.2 (CBMAI 1427=CBS 12681) and Spathaspora xylofermentans sp. nov. UFMG-HMD23.3 (=CBMAI 1428=CBS 12682). PMID:23053696

Cadete, Raquel M; Melo, Monaliza A; Zilli, Jerri E; Vital, Marcos J S; Mouro, Adriane; Prompt, Alice H; Gomes, Fátima C O; Stambuk, Boris U; Lachance, Marc-André; Rosa, Carlos A

2012-10-05

258

Uranium accumulation by immobilized cells of a Citrobacter sp  

Microsoft Academic Search

Uranium was removed from challenge flows presented to immobilized cells of aCitrobacter sp. In excess of 90% of the presented metal was recovered, giving high yields of accumulated metal which could be subsequently released from the immobilized cellsin situ.

Lynne E. Macaskie; A. C. R. Dean

1985-01-01

259

Staphylococcus piscifermentans sp. nov., from fermented fish in Thailand.  

PubMed

New coagulase-negative staphylococci were isolated from fermented fish in Thailand. These organisms were differentiated from other Staphylococcus species on the basis of DNA relatedness and biochemical characteristics. Staphylococcus piscifermentans sp. nov. is described, and the type strain is strain SK03 (= NRIC 1817 = JCM 6057 = TISTR 824). PMID:1390108

Tanasupawat, S; Hashimoto, Y; Ezaki, T; Kozaki, M; Komagata, K

1992-10-01

260

Preliminary site design for the SP100 ground engineering test  

Microsoft Academic Search

In November, 1985, Hanford was selected by the Department of Energy (DOE) as the preferred site for a full-scale test of the integrated nuclear subsystem for SP-100. The Hanford Engineering Development Laboratory, operated by Westinghouse Hanford Company, was assigned as the lead contractor for the Test Site. The nuclear subsystem, which includes the reactor and its primary heat transport system,

C. M. Cox; W. C. Miller; M. K. Mahaffey

1986-01-01

261

SP100 early demonstration flight system design and testing  

Microsoft Academic Search

A Space Nuclear Power System design for an early flight application has been developed focused on SP-100 technology currently available. This 10 kWe class space power plant uses a fast spectrum Li cooled reactor coupled to the highly successful thermoelectric converters which have flown on the Galileo and Ulysses missions. The concept is characterized by low mass, high reliability, and

Neil W. Brown; Hwang Choe; Stanley M. Davies; Aaron S. Kirpich

1993-01-01

262

Even extreme heat cannot affect the SP-ablator  

NASA Astrophysics Data System (ADS)

A heat-protection system that was successfully tested in a plasma wind tunnel is described. The system is capable of withstanding extreme short-term heat loads when reentering the atmosphere. The surface-protected (SP) ablator exhibits significant increase in the efficiency of the cooling effect and dynamic pressure resistance due to a ceramic matrix composite layer that protects its surface.

Stoclfleth, Holger; Knabe, Helmut; Wahl, Juergen; Haug, Tilman

263

Production of extracellular water-insoluble polysaccharide from Pseudomonas sp.  

PubMed

Curdlan is a microbial polysaccharide composed exclusively of ?-(1,3)-linked glucose residues. Until now only bacteria belonging to the Alcaligenes and Agrobacterium species have been reported to produce Curdlan. In this study, a bacterium capable of producing extracellular Curdlan, identified as Pseudomonas sp. on the basis of 16S rDNA gene sequencing, was isolated from soil samples. From the HPLC, permethylation linkage analysis, (13)C NMR, and FT-IR analytical data, the polysaccharide consisted exclusively of glucose; the most prominent sugar was 1,3-linked glucose, and most glycosidic bonds joining these sugar residues were of the ?-type. This also supported that the exopolysaccharide produced by Pseudomonas sp. was actually Curdlan. In addition, the Pseudomonas sp. was studied for the production of Curdlan by conventional "one-factor-at-a-time technique" and response surface methodology (RSM). It was observed that glucose and yeast extract were the most suitable carbon source and nitrogen source for Curdlan production, respectively. By using RSM, Curdlan production was increased significantly by 188%, from 1.25 to 2.35 g/L, when the strain was cultivated in the optimal condition developed by RSM, and the highest Curdlan production rate of 0.81 g/(L h) was obtained. To the best of the authors' knowledge, this is the first report on Curdlan production by Pseudomonas sp. PMID:22533491

Cui, Jian-Dong; Qiu, Ji Qing

2012-05-04

264

Evaluation of integral measurements for the SP-100 space reactor  

NASA Astrophysics Data System (ADS)

Experiments in support of the SP-100 space reactor were done in the Zero Power Physics Reactor-20 (ZPPR-20). Biases in calculations were found for criticality predictions and control worths. Although the biases were on the conservative side for SP-100, the more general question of the reasons for the calculational errors is important for design optimization and for other fast space reactor designs. A data sensitivity analysis was made for the ZPPR-20 results using the GMADJ code. Results for ZPPR-20 are used in comparison with a wide database of fast reactor parameters. It is shown that calculations of criticality for the reference core, for a core simulating water immersion, and for the control rod worths are consistent with all other benchmark data and that differences in prediction are within uncertainties in nuclear data. The results from ZPPR-20 can be used with confidence for prediction of calculational bias factors and uncertainties in SP-100 and related designs. The most effective use of the ZPPR-20 data would be to create a sensitivity file for SP-100 and use the GMADJ system to produce biases and uncertainties.

Collins, P. J.; Grasseschi, G. L.; Aumeier, S. E.

265

Genome Sequence of Alcaligenes sp. Strain HPC1271  

PubMed Central

We report a draft genome sequence of Alcaligenes sp. strain HPC1271, which demonstrates antimicrobial activity against multidrug-resistant bacteria. Antibiotic production by Alcaligenes has not been frequently reported, and hence, the availability of the genome sequence should enable us to explore new antibiotic-producing gene clusters.

Sagarkar, Sneha; Tanksale, Himgouri; Sharma, Nandita; Qureshi, Asifa; Khardenavis, Anshuman; Purohit, Hemant J.

2013-01-01

266

Users guide to the Argonne SP scheduling system  

Microsoft Academic Search

During the past five years scientists discovered that modern UNIX workstations connected with ethernet and fiber networks could provide enough computational performance to compete with the supercomputers of the day. As this concept became increasingly popular, the need for distributed queuing and scheduling systems became apparent. Today, supercomputers, such as Argonne National Laboratory`s IBM SP system, can provide more CPU

David A. Lifka; Mark W. Henderson; Karen Rayl

1995-01-01

267

Resistance to Fusarium oxysporum f.sp. lilii in Lilium  

Microsoft Academic Search

The soil-born fungus, Fusarium oxysporum f. sp. lilii, causes bulb rot in lilies, which endangers its bulb production worldwide. The resistance of 44 species against Fusarium was scored under standardized test conditions. The Asiatic cultivars 'Orlito' and 'Connecticut King' were highly resistant, while 'Pirate' was highly susceptible. The resistance level of the Oriental hybrids such as 'Acapulco' and 'Stargazer' and

J. H. Lim; H. K. Rhee; Y. J. Kim; K. B. Lim; J. M. van Tuyl

268

Characterization of phenanthrene degradation by strain polyporus sp. S133.  

PubMed

Polyporus sp. S133, a fungus collected from contaminated soil, was used to degrade phenanthrene, a polycyclic aromatic hydrocarbon, in a mineral salt broth liquid culture. A maximal degradation rate (92%) was obtained when Polyporus sp. S133 was cultured for 30 days with agitation at 120 r/min, as compared to 44% degradation in non-agitated cultures. Furthermore, the degradation was affected by the addition of surfactants. Tween 80 was the most suitable surfactant for the degradation of phenanthrene by Polyporus sp. S133. The degradation rate increased as the amount of Tween 80 added increased. The rate in agitated cultures was about 2 times that in non-agitated cultures. The mechanism of degradation was determined through the identification of metabolites; 9,10-phenanthrenequinone, 2,2'-diphenic acid, phthalic acid, and protocatechuic acid. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Polyporus sp. S133 were detected during the incubation. The highest level of activity was shown by 1,2-dioxygenase (187.4 U/L) after 20 days of culture. PMID:20397398

Hadibarata, Tony; Tachibana, Sanro

2010-01-01

269

Sputtering studies with the Monte Carlo Program TRIM.SP  

Microsoft Academic Search

The Monte Carlo Program TRIM.SP (sputtering version of TRIM) was used to determine sputtering yields and energy and angular distributions of sputtered particles in physical (collisional) sputtering processes. The output is set up to distinguish between the contributions of primary and secondary knock-on atoms as caused by in- and outgoing incident ions, in order to get a better understanding of

J. P. Biersack; W. Eckstein

1984-01-01

270

Die Aufnahme partikulärer Nahrung bei Reniera sp. (Porifera)  

Microsoft Academic Search

The choanocyte chambers of the marine sponge Reniera sp. protrude with their curved outer surface free into the incurrent canals. The water is sucked into the chambers by cavities between the choanocytes. Particles up to 1 µm in diameter may enter the chambers with the water current. These particles are trapped on the outer surface of the choanocyte collars and

Paul-Friedrich Langenbruch

1985-01-01

271

Die Aufnahme partikulärer Nahrung bei Reniera sp. (Porifera)  

Microsoft Academic Search

The choanocyte chambers of the marine spongeReniera sp. protrude with their curved outer surface free into the incurrent canals. The water is sucked into the chambers by cavities between the choanocytes. Particles up to 1 µm in diameter may enter the chambers with the water current. These particles are trapped on the outer surface of the choanocyte collars and are

Paul-Friedrich Langenbruch

1985-01-01

272

Complete Genome Sequence of Antarctic Bacterium Psychrobacter sp. Strain G.  

PubMed

Here, we report the complete genome sequence of Psychrobacter sp. strain G, isolated from King George Island, Antarctica, which can produce lipolytic enzymes at low temperatures. The genomics information of this strain will facilitate the study of the physiology, cold adaptation properties, and evolution of this genus. PMID:24051316

Che, Shuai; Song, Lai; Song, Weizhi; Yang, Meng; Liu, Guiming; Lin, Xuezheng

2013-09-19

273

cDNAs from Nylanderia sp nr pubens (Hymenoptera: Formicidae)  

Technology Transfer Automated Retrieval System (TEKTRAN)

7 new gene sequences were identified from workers of Rasberry crazy ant, Nylanderia sp.nr. pubens, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are HQ636472-HQ636478. This information will provide scientists with genetic tools to study the pop...

274

Genome Sequence of the Edible Cyanobacterium Arthrospira sp. PCC 8005?  

PubMed Central

We determined the genome sequence of Arthrospira sp. PCC 8005, a cyanobacterial strain of great interest to the European Space Agency for its nutritive value and oxygenic properties in the Micro-Ecological Life Support System Alternative (MELiSSA) biological life support system for long-term manned missions into space.

Janssen, P. J.; Morin, N.; Mergeay, M.; Leroy, B.; Wattiez, R.; Vallaeys, T.; Waleron, K.; Waleron, M.; Wilmotte, A.; Quillardet, P.; de Marsac, N. Tandeau; Talla, E.; Zhang, C.-C.; Leys, N.

2010-01-01

275

Genome sequence of the edible cyanobacterium Arthrospira sp. PCC 8005.  

PubMed

We determined the genome sequence of Arthrospira sp. PCC 8005, a cyanobacterial strain of great interest to the European Space Agency for its nutritive value and oxygenic properties in the Micro-Ecological Life Support System Alternative (MELiSSA) biological life support system for long-term manned missions into space. PMID:20233937

Janssen, P J; Morin, N; Mergeay, M; Leroy, B; Wattiez, R; Vallaeys, T; Waleron, K; Waleron, M; Wilmotte, A; Quillardet, P; de Marsac, N Tandeau; Talla, E; Zhang, C-C; Leys, N

2010-03-16

276

Germination of Achira Seed (Canna sp.) Approximately 550 Years Old  

Microsoft Academic Search

DURING the excavation of a tomb at the archaeological site of Santa Rosa de Tastil, Argentina (24° 25' S., 65° 50' W.), we found a necklace made out of nuts of Juglans australis. Inside each nut was a seed of Canna sp., making a rattle. Samples from bones of cameloids in the upper strata of garbage from the site were

E. Sivori; F. Nakayama; E. Cigliano

1968-01-01

277

Production of Thermostable ? Amylase and Cellulase from Cellulomonas sp  

Microsoft Academic Search

A bacterium, isolated from rabbit's waste and identified as Cellulomonas sp., had cellulase and thermostable ? -amylase activity when grown on wheat bran. Maximum activity of thermostable ? -amylase was obtained by adding 3% soluble starch. However, soybean oil (1 ml l - 1 ) could increase the production of ? -amylase and cellulase in wheat bran. The ? -amylase

G. EMTIAZI; I. NAHVI

2004-01-01

278

Association of Bursaphelenchus sp. (Nematoda : Aphefenchoididae) with nitidulid beetles (Coleoptera : Nitidulidae)  

Microsoft Academic Search

SUMMARY Dauer juveniles (JIII) of an undescribed Bursaphelenchus sp. were recovered from the median oviduct and ovipositor sac of adult females and the interna1 sac of adult males of the pineapple beetle, Urophorus humeralis. This nematode was reared on cultures of the fungi Moniliniafructicola or Penicillium sp. In experimental studies, dauers (dispersal stage) of Bursaphelenchus sp. could be recovered intemally

Robin M. GIBLIN

279

Subterminal oxidation of n-alkanes in achlorophyllous alga Prototheca sp.  

PubMed

Some Prototheca sp. are known to be involved in n-hexadecane degradation. Two derivatives derived from n-hexadecane in such Prototheca sp. were identified as 5-hexadecanone and 5-hexadecanol. n-Hexadecane was assumed to be converted to 5-hexadecanol and then to 5-hexadecanone through a unique subterminal oxidation pathway in such Prototheca sp. PMID:23651808

Sakuradani, Eiji; Natsume, Yusuke; Takimura, Yasushi; Ogawa, Jun; Shimizu, Sakayu

2013-05-04

280

Endophytic actinomycetes from Pinus thunbergii and their antifungal activity against Cylindrocladium sp  

Microsoft Academic Search

The inside of Pinus thunbergii could be a reliable screening source for a useful agent in controlling plant disease. Isolation of endophytic actinomycetes from P. thunbergii and their potential as biocontrol agents against the plant pathogen Cylindrocladium sp. were investigated. Two endophytic actinomycetes, Streptomyces sp. and Microbispora sp., were isolated from surface-sterilised root tissues of P. thunbergii seedlings. The recovery

Ryota Kataoka; Kazuyoshi Futai

2011-01-01

281

Direct fermentation of cellulose to ethanol by a cellulolytic filamentous fungus, Monilia sp  

Microsoft Academic Search

A saprophytic filamentous fungus, Monilia sp., isolated from bagasse compost was found to utilize many polysaccharides (including cellulose) and to produce cellulases and hemicellulases. Monilla sp. also fermented glucose, xylose and cellulosic materials to ethanol. Over 60% of the solid cellulose substrate added to Monilia sp. cultures was converted to ethanol as the major fermentation product. These results indicate that

Cheng-shung Gong; Christine M. Maun; George T. Tsao

1981-01-01

282

IDENTIFICATION AND MOLECULAR CLONING OF XENOPUS LAEVIS SP22, A PROTEIN ASSOCIATED WITH FERTILIZATION IN MAMMALS  

EPA Science Inventory

ABSTRACT SP22 is a protein that has been characterized in rats where it has been related with fertility. SP22 homologues have been studied in mouse and man and a definitive role for the protein has not been assigned yet. By means of a polyclonal IgG to recombinant rat SP22...

283

Sp1/Sp3 and DNA-methylation contribute to basal transcriptional activation of human podoplanin in MG63 versus Saos-2 osteoblastic cells  

PubMed Central

Background Podoplanin is a membrane mucin that, among a series of tissues, is expressed on late osteoblasts and osteocytes. Since recent findings have focussed on podoplanin's potential role as a tumour progression factor, we aimed at identifying regulatory elements conferring PDPN promoter activity. Here, we characterized the molecular mechanism controlling basal PDPN transcription in human osteoblast-like MG63 versus Saos-2 cells. Results We cloned and sequenced 2056 nucleotides from the 5'-flanking region of the PDPN gene and a computational search revealed that the TATA and CAAT box-lacking promoter possesses features of a growth-related gene, such as a GC-rich 5' region and the presence of multiple putative Sp1, AP-4 and NF-1 sites. Reporter gene assays demonstrated a functional promoter in MG63 cells exhibiting 30-fold more activity than in Saos-2 cells. In vitro DNase I footprinting revealed eight protected regions flanked by DNaseI hypersensitive sites within the region bp -728 to -39 present in MG63, but not in Saos-2 cells. Among these regions, mutation and supershift electrophoretic mobility shift assays (EMSA) identified four Sp1/Sp3 binding sites and two binding sites for yet unknown transcription factors. Deletion studies demonstrated the functional importance of two Sp1/Sp3 sites for PDPN promoter activity. Overexpression of Sp1 and Sp3 independently increased the stimulatory effect of the promoter and podoplanin mRNA levels in MG63 and Saos-2 cells. In SL2 cells, Sp3 functioned as a repressor, while Sp1 and Sp3 acted positively synergistic. Weak PDPN promoter activity of Saos-2 cells correlated with low Sp1/Sp3 nuclear levels, which was confirmed by Sp1/Sp3 chromatin immunoprecipitations in vivo. Moreover, methylation-sensitive Southern blot analyses and bisulfite sequencing detected strong methylation of CpG sites upstream of bp -464 in MG63 cells, but hypomethylation of these sites in Saos-2 cells. Concomitantly, treatment with the DNA methyltransferase inhibitor 5-azaCdR in combination with trichostatin A (TSA) downregulated podoplanin mRNA levels in MG63 cells, and region-specific in vitro methylation of the distal promoter suggested that DNA methylation rather enhanced than hindered PDPN transcription in both cell types. Conclusion These data establish that in human osteoblast-like MG63 cells, Sp1 and Sp3 stimulate basal PDPN transcription in a concerted, yet independent manner, whereas Saos-2 cells lack sufficient nuclear Sp protein amounts for transcriptional activation. Moreover, a highly methylated chromatin conformation of the distal promoter region confers cell-type specific podoplanin upregulation versus Saos-2 cells.

Hantusch, Brigitte; Kalt, Romana; Krieger, Sigurd; Puri, Christina; Kerjaschki, Dontscho

2007-01-01

284

Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations  

Microsoft Academic Search

Two strains of Acetobacter sp., LTH 2460T and LTH 2458T, have been isolated from running red wine and cider vinegar fermentations, respectively. Taxonomic characteristics of the isolates were investigated. Comparative analysis of the 165 rRNA sequences revealed > 99% similarity between strain LTH 2460T and the type strains of the related species Acetobacter europaeus and Acetobacter xylinus and between strain

Stephan J. Sokollek; C. Hertel; Walter P. Hammes

1998-01-01

285

Hydrolysis of surimi wastewater for production of transglutaminase by Enterobacter sp. C2361 and Providencia sp. C1112.  

PubMed

Surimi wastewater (SWW) is an industrial wastewater, released during the washing step of surimi preparation from minced fish, that causes environmental problem. In this study, SWW produced from ornate threadfin bream (Nemipterus hexodon) was hydrolysed and used to cultivate Enterobacter sp. C2361 and Providencia sp. C1112 for the production of microbial transglutaminase (MTGase, EC 2.3.2.13). The SWW was repeatedly used to wash the fish mince that gained a final protein content of 3.20% (w/v). The commercial protease, Delvolase was the most appropriate protease used to produce fish protein hydrolysate (FPH) from SWW. The FPH at 40% degree of hydrolysis was used instead of a peptone portion in the SPY medium (3.0% starch, 2.0% peptone, 0.2% yeast extract, 0.2% MgSO(4), 0.2% K(2)HPO(4) and 0.2% KH(2)HPO(4), pH 7.0) to cultivate the tested strains at 37°C, shaking speed at 150rpm. Providencia sp. C1112 produced higher MTGase activity (1.78±0.05U/ml) than Streptoverticillium mobaraense (1.61±0.02U/ml) at 18h of cultivation in FPH medium. On the other hand, the Enterobacter sp. C2361 produced lower MTGase activity (1.18±0.03U/ml). PMID:22953841

H-Kittikun, Aran; Bourneow, Chaiwut; Benjakul, Soottawat

2012-05-22

286

Vibrio sp. as a potentially important member of the Black Band Disease (BBD) consortium in Favia sp. corals.  

PubMed

Black Band Disease (BBD) is a well-described disease plaguing corals worldwide. It has been established that ecological and environmental stress factors contribute to the appearance and progression of the disease, believed to be caused by a diverse microbial consortium. We have identified and characterized Vibrio sp. associated with BBD in Eilat reef corals using both culture-dependent and -independent methods. Direct sampling using 16S rRNA gene clone libraries showed seasonal dynamics in the diversity of BBD-associated Vibrios. In the two sampling periods, BBD-associated Vibrio clones showed similarities to different groups: October samples were similar to known pathogens, while December samples were similar to general aquatic Vibrio sp. Cultured bacterial isolates of Vibrio sp. were highly homologous (>or=99%) to previously documented BBD-associated bacteria from the Caribbean, Bahamas and Red Seas, and were similar to several known coral pathogens, such as Vibrio coralliilyticus. The proteolytic activity of Vibrio sp., as measured using casein- and azocasein-based assays, directly correlated with temperature elevation and peaked at 26-28 degrees C, with the microorganisms producing more proteases per bacterial cell or increasing the rate of proteolytic activity of the same proteases (potentially metalloproteases). This activity may promote coral tissue necrosis and aid in ensuing progression of the coral BBD. PMID:19780825

Arotsker, Luba; Siboni, Nachshon; Ben-Dov, Eitan; Kramarsky-Winter, Esti; Loya, Yossi; Kushmaro, Ariel

2009-08-24

287

The ‘inverted ladder’ orb web of Scoloderus sp. and the intermediate orb of Eustala (?) sp. Araneae: Araneidae  

Microsoft Academic Search

The South American spider Scoloderus sp. spins a remarkably elongated ‘ladder’ orb web similar to that of an as yet unidentified New Guinean spider except that it is inverted 180°, with the long part above rather than below the hub. The two webs differ in a number of details, some of which may represent alternate solutions to the special problems

William G. Eberhard

1975-01-01

288

Whole-genome sequence of Enterobacter sp. strain SST3, an endophyte isolated from Jamaican sugarcane (Saccharum sp.) stalk tissue.  

PubMed

Enterobacter sp. strain SST3 is an endophytic bacterium isolated from Saccharum spp. Here we present its annotated draft genome that may shed light on its role as a bacterial endophyte of sugarcane. To our knowledge, this is the first genome announcement of a sugarcane-associated bacterium from the genus Enterobacter. PMID:23045495

Gan, Han Ming; McGroty, Sean E; Chew, Teong Han; Chan, Kok Gan; Buckley, Larry J; Savka, Michael A; Hudson, André O

2012-11-01

289

Acrobeloides ishraqi sp. n. and Acrobeloides mushtaqi sp. n. (Nematoda: Rhabditida) from chickpea rhizosphere, Uttar Pradesh, India  

Microsoft Academic Search

Two new species of nematode in the genus Acrobeloides were found from rhizosphere of chickpea from Jhansi and Faizabad districts of Uttar Pradesh, India. Acrobeloides ishraqi sp. n. and A. mushtaqi were identified and described on the basis of morphological observations. Measurement and morphological observation of different stages of A. ishraqi were taken and compared with known species of the

Rashid Pervez

2011-01-01

290

Neotobrilus nicsmolae n. sp. (Tobrilidae: Nematoda) and Chronogaster carolinensis n. sp. (Chronogasteridae: Nematoda) from Lake Phelps, North Carolina.  

PubMed

Two new species, Neotobrilus nicsmolae n. sp. and Chronogaster carolinensis n. sp. are described from a small, acidic, temperate, natural lake in North Carolina. N. nicsmolae n. sp. comes close to three members of the genus reported from North America, N. filipjevi, N. longus, and N. hopei. However, N. nicsmolae is unique with in the genus in having a combination of characters: size smaller than 1,700 ?m, shorter outer labial and cephalic setae, tail shorter than 250 ?m, last ventromedian supplement close (about 5 ?m) to cloacal opening, spicule length of 61 to 85 ?m, flagelloid sperm, and possession of subterminal setae. Assessment of relationships among clades within the Triplonchida using DNA sequences of the D2D3 expansion segment of the LSU rDNA showed that the family Trichodoridae and the genus Tripyla were recovered as monophyletic. The genus Tobrilus was recovered as monophyletic in the neighbor-joining and maximum likelihood trees, but that was not so in the maximum-parsimony tree. The separation among genera of the Trichodoridae, i.e., Trichodorus and Paratrichodorus, was not clear-cut in all phylograms. Chronogaster carolinensis n. sp. in having one ventral mucro with no spine and vacuolated lateral glandular bodies comes close to C. typica and C. ethiopica but differs from all hitherto known species in a combination of characteristics: in having long cephalic setae, long stoma, crystalloid bodies, vacuolated lateral glandular bodies, and a tail terminus with blunt ventral mucro, and its lack of lateral line. PMID:23589662

Abebe, Eyualem; Ferebee, Briana; Taylor, Tarreyca; Mundo-Ocampo, Manuel; Mekete, Tesfamariam; De Ley, Paul

2013-03-01

291

Phylogenetic analysis of methanogenic enrichment cultures obtained from Lonar Lake in India: isolation of Methanocalculus sp. and Methanoculleus sp.  

PubMed

The diversity of methanogenic archaea in enrichment cultures established from the sediments of Lonar Lake (India), a soda lake having pH approximately 10, was investigated using 16S rDNA molecular phylogenetic approach. Methanogenic enrichment cultures were developed in a medium that simulated conditions of soda lake with three different substrates viz., H(2):CO(2), sodium acetate, and trimethylamine (TMA), at alkaline pH. Archaeal 16S rRNA clone libraries were generated from enrichment cultures and 13 RFLP groups were obtained. Representative sequence analysis of each RFLP group indicated that the majority of the 16S rRNA gene sequences were phylogenetically affiliated with uncultured Archaea. Some of the groups may belong to new archaeal genera or families. Three RFLP groups were related to Methanoculleus sp, while two related to Methanocalculus sp. 16S rRNA gene sequences found in Lonar Lake were different from sequences reported from other soda lakes and more similar to those of oil reservoirs, palm oil waste treatment digesters, and paddy fields. In culture-based studies, three isolates were obtained. Two of these were related to Methanoculleus sp. IIE1 and one to Methanocalculus sp. 01F97C. These results clearly show that the Lonar Lake ecosystem harbors unexplored methanogens. PMID:17483868

Surakasi, Venkata Prasad; Wani, Aijaz Ahmad; Shouche, Yogesh S; Ranade, Dilip R

2007-05-05

292

Identification and Characterization of Spontaneous Deletions within the Sp11-Sp12 Prophage Region of Escherichia coli O157:H7 Sakai  

PubMed Central

Prophages make up 12% of the enterohemorrhagic Escherichia coli genome and play prominent roles in the evolution and virulence of this food-borne pathogen. Acquisition and loss of and rearrangements within prophage regions are the primary causes of differences in pulsed-field gel electrophoresis (PFGE) patterns among strains of E. coli O157:H7. Sp11 and Sp12 are two tandemly integrated and putatively defective prophages carried by E. coli O157:H7 strain Sakai. In this study, we identified 3 classes of deletions that occur within the Sp11-Sp12 region, at a frequency of ca. 7.74 × 10?4. One deletion resulted in a precise excision of Sp11, and the other two spanned the junction of Sp11 and Sp12. All deletions resulted in shifts in the XbaI fragment pattern observed by PFGE. We sequenced the inducible prophage pool of Sakai but did not identify any mature phage particles corresponding to either Sp11 or Sp12. Deletions containing pchB and psrC, which are Sp11-carried genes encoding proteins known or suspected to regulate type III secretion, did not affect the secretion levels of the EspA or EspB effector. Alignment of the Sp11-Sp12 DNA sequence with its corresponding regions in other E. coli O157:H7 and O55:H7 strains suggested that homologous recombination rather than integrase-mediated excision is the mechanism behind these deletions. Therefore, this study provides a mechanism behind the previously observed genetic instability of this genomic region of E. coli O157:H7.

Chen, Chun; Lewis, Carrie R.; Goswami, Kakolie; Roberts, Elisabeth L.; DebRoy, Chitrita

2013-01-01

293

Membrane fatty acids adaptive profile in the simultaneous presence of arsenic and toluene in Bacillus sp. ORAs2 and Pseudomonas sp. ORAs5 strains  

Microsoft Academic Search

Bacillus sp. ORAs2 and Pseudomonas sp. ORAs5, two arsenic-resistant bacterial strains previously isolated from sediments of the Orbetello Lagoon, Italy, were\\u000a tested for their adaptation to mixed contaminants on the level of membrane fatty acid composition. The two bacterial strains\\u000a were characterized by high levels of arsenic resistance, and Pseudomonas sp. ORAs5 was also shown to be solvent-tolerant. The bacterial

Milva Pepi; Hermann J. Heipieper; Janett Fischer; Marcella Ruta; Margherita Volterrani; Silvano E. Focardi

2008-01-01

294

Cisplatin in 5% Ethanol Eradicates Cisplatin-Resistant Lung Tumor by Killing Lung Cancer Side Population (SP) Cells and Non-SP Cells.  

PubMed

Cancer side population (SP) cells with cancer stem cell-like properties are thought to be responsible for lung cancer chemotherapy resistance and currently no drug can efficiently target them. Breast cancer resistance protein (BRCP/ABCG2) is a major drug transporter in protecting lung cancer SP cells from cytotoxic agents. We showed that a low concentration of ethanol, which inhibits many membrane proteins, inhibits ABCG2 in lung cancer SP cells. Furthermore, cytotoxic cisplatin (DDP) in 5% (vol/vol) ethanol kills SP plus non-SP cancer cells better than either treatment alone in eradicating chemoresistant lung tumors. We found that 5% ethanol did not reduce ABCG2 protein levels, but significantly reduced ABCG2 protein function by a Hoechst 33342 extrusion assay, an ATPase activity assay, and transmission electron microscopy. Further, DDP in 5% ethanol (5% ethanol-DDP) induced apoptosis of the SP plus non-SP cancer cells both in vitro and in vivo. In DDP-resistant A549/DDP lung tumor-bearing Balb/C nude mice, intratumoral injection of 5% ethanol-DDP regressed tumors and significantly improved survivals compared with 5% ethanol, DDP alone, or control. Intratumoral injection of 5% ethanol-DDP helped eradicate tumors in 30% (3/10) of the mice after 4 weeks treatment. By killing SP and non-SP cancer cells, 5% ethanol-DDP could eradicate DDP-resistant lung tumor and extend survival, providing a novel way to improve chemoresistant lung cancer survival for clinic. PMID:24009622

Niu, Qi; Wang, Wei; Li, Yong; Ruden, Douglas M; Li, Qian; Wang, Fenghua

2013-08-29

295

Cobryketone derived from vitamin B12 via palladium-catalyzed cleavage of the sp3-sp3 carbon-carbon bond.  

PubMed

Heptamethyl cobyrinate was transformed into hexamethyl 8-nor-cobyrinate. The crucial step involved the synthesis of new, vitamin B12 derived cobryketone via palladium-catalyzed cleavage of the sp(3)-sp(3) carbon-carbon bond with the liberation of the ketone. The replacement of sp(3) carbon atom with sp(2) (C?O) at the 8-position produces a bathochromic shift of all absorption bands and makes ? and ? bands equal as a consequence of the expansion of the existing conjugated system of double bonds. PMID:23544362

Kurco?, Sylwester; Proinsias, Keith ó; Gryko, Dorota

2013-04-10

296

Halobellus limi sp. nov. and Halobellus salinus sp. nov., isolated from two marine solar salterns.  

PubMed

Two halophilic archaea, strains TBN53(T) and CSW2.24.4(T), were characterized to elucidate their taxonomic status. Strain TBN53(T) was isolated from the Taibei marine solar saltern near Lianyungang city, Jiangsu province, China, whereas strain CSW2.24.4(T) was isolated from a saltern crystallizer in Victoria, Australia. Cells of the two strains were pleomorphic, stained Gram-negative and produced red-pigmented colonies. Strain TBN53(T) was able to grow at 25-55 °C (optimum 45 °C), with 1.4-5.1 M NaCl (optimum 2.6-3.9 M NaCl), with 0-1.0 M MgCl(2) (optimum 0-0.1 M MgCl(2)) and at pH 5.5-9.5 (optimum pH 7.0), whereas strain CSW2.24.4(T) was able to grow at 25-45 °C (optimum 37 °C), with 2.6-5.1 M NaCl (optimum 3.4 M NaCl), with 0.01-0.7 M MgCl(2) (optimum 0.05 M MgCl(2)) and at pH 5.5-9.5 (optimum pH 7.0-7.5). Cells of the two isolates lysed in distilled water. The minimum NaCl concentrations that prevented cell lysis were 8 % (w/v) for strain TBN53(T) and 12 % (w/v) for strain CSW2.24.4(T). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate, with two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. Trace amounts of other unidentified lipids were also detected. On the basis of 16S rRNA gene sequence analysis, strains TBN53(T) and CSW2.24.4(T) showed 94.1 % similarity to each other and were closely related to Halobellus clavatus TNN18(T) (95.0 and 94.7 % similarity, respectively). Levels of rpoB' gene sequence similarity between strains TBN53(T) and CSW2.24.4(T), and between these strains and Halobellus clavatus TNN18(T) were 88.5, 88.5 and 88.1 %, respectively. The DNA G+C contents of strains TBN53(T) and CSW2.24.4(T) were 69.2 and 67.0 mol%, respectively. The level of DNA-DNA relatedness between strain TBN53(T) and strain CSW2.24.4(T) was 25 %, and these two strains showed low levels of DNA-DNA relatedness with Halobellus clavatus TNN18(T) (30 and 29 % relatedness, respectively). Based on these phenotypic, chemotaxonomic and phylogenetic properties, two novel species of the genus Halobellus are proposed to accommodate these two strains, Halobellus limi sp. nov. (type strain TBN53(T) = CGMCC 1.10331(T) = JCM 16811(T)) and Halobellus salinus sp. nov. (type strain CSW2.24.4(T) = DSM 18730(T) = CGMCC 1.10710(T) = JCM 14359(T)). PMID:22661071

Cui, Heng-Lin; Yang, Xin; Zhou, Yu-Guang; Liu, Hong-Can; Zhou, Pei-Jin; Dyall-Smith, Mike L

2012-06-01

297

Characterization of a Streptococcus sp.-Veillonella sp. Community Micromanipulated from Dental Plaque?  

PubMed Central

Streptococci and veillonellae occur in mixed-species colonies during formation of early dental plaque. One factor hypothesized to be important in assembly of these initial communities is coaggregation (cell-cell recognition by genetically distinct bacteria). Intrageneric coaggregation of streptococci occurs when a lectin-like adhesin on one streptococcal species recognizes a receptor polysaccharide (RPS) on the partner species. Veillonellae also coaggregate with streptococci. These genera interact metabolically; lactic acid produced by streptococci is a carbon source for veillonellae. To transpose these interactions from undisturbed dental plaque to an experimentally tractable in vitro biofilm model, a community consisting of RPS-bearing streptococci juxtaposed with veillonellae was targeted by quantum dot-based immunofluorescence and then micromanipulated off the enamel surface and cultured. Besides the expected antibody-reactive cell types, a non-antibody-reactive streptococcus invisible during micromanipulation was obtained. The streptococci were identified as Streptococcus oralis (RPS bearing) and Streptococcus gordonii (adhesin bearing). The veillonellae could not be cultivated; however, a veillonella 16S rRNA gene sequence was amplified from the original isolation mixture, and this sequence was identical to the sequence of the previously studied organism Veillonella sp. strain PK1910, an oral isolate in our culture collection. S. oralis coaggregated with S. gordonii by an RPS-dependent mechanism, and both streptococci coaggregated with PK1910, which was used as a surrogate during in vitro community reconstruction. The streptococci and strain PK1910 formed interdigitated three-species clusters when grown as a biofilm using saliva as the nutritional source. PK1910 grew only when streptococci were present. This study confirms that RPS-mediated intrageneric coaggregation occurs in the earliest stages of plaque formation by bringing bacteria together to create a functional community.

Chalmers, Natalia I.; Palmer, Robert J.; Cisar, John O.; Kolenbrander, Paul E.

2008-01-01

298

Interaction of Pulmonary Surfactant Protein SP-A with DPPC/Egg-PG Bilayers  

PubMed Central

In the mixture of lipids and proteins which comprise pulmonary surfactant, the dominant protein by mass is surfactant protein A (SP-A), a hydrophilic glycoprotein. SP-A forms octadecamers that interact with phospholipid bilayer surfaces in the presence of calcium. Deuterium NMR was used to characterize the perturbation by SP-A, in the presence of 5 mM Ca2+, of dipalmitoyl phosphatidylcholine (DPPC) properties in DPPC/egg-PG (7:3) bilayers. Effects of SP-A were uniformly distributed over the observed DPPC population. SP-A reduced DPPC chain orientational order significantly in the gel phase but only slightly in the liquid-crystalline phase. Quadrupole echo decay times for DPPC chain deuterons were sensitive to SP-A in the liquid-crystalline mixture but not in the gel phase. SP-A reduced quadrupole splittings of DPPC choline ?-deuterons but had little effect on choline ?-deuteron splittings. The observed effects of SP-A on DPPC/egg-PG bilayer properties differ from those of the hydrophobic surfactant proteins SP-B and SP-C. This is consistent with the expectation that SP-A interacts primarily at bilayer surfaces.

Morrow, Michael R.; Abu-Libdeh, Nidal; Stewart, June; Keough, Kevin M. W.

2003-01-01

299

Role of zinc finger structure in nuclear localization of transcription factor Sp1  

SciTech Connect

Transcription factor Sp1 is localized in the nucleus and regulates gene expression. Our previous study demonstrated that the carboxyl terminal region of Sp1 containing 3-zinc finger region as DNA binding domain can also serve as nuclear localization signal (NLS). However, the nuclear transport mechanism of Sp1 has not been well understood. In this study, we performed a gene expression study on mutant Sp1 genes causing a set of amino acid substitutions in zinc finger domains to elucidate nuclear import activity. Nuclear localization of the GFP-fused mutant Sp1 proteins bearing concomitant substitutions in the first and third zinc fingers was highly inhibited. These mutant Sp1 proteins had also lost the binding ability as to the GC box sequence. The results suggest that the overall tertiary structure formed by the three zinc fingers is essential for nuclear localization of Sp1 as well as dispersed basic amino acids within the zinc fingers region.

Ito, Tatsuo; Azumano, Makiko [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Uwatoko, Chisana [Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, 610-0395 (Japan); Itoh, Kohji [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan)], E-mail: kitoh@ph.tokushima-u.ac.jp; Kuwahara, Jun [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo, Kyotanabe City, 610-0395 (Japan)], E-mail: jkuwahar@dwc.doshisha.ac.jp

2009-02-27

300

Sub-lethal and chronic salinity tolerances of three freshwater insects: Cloeon sp. and Centroptilum sp. (Ephemeroptera: Baetidae) and Chironomus sp. (Diptera: Chironomidae).  

PubMed

Increased salinity in rivers and streams is a serious environmental concern, and in Australia there is growing information about the acute tolerances to salinity for freshwater macroinvertebrates, but much less information about chronic and sub-lethal tolerances. The effects of increased salinity on the growth and survival of two mayflies, Cloeon sp. and Centroptilum sp. and one midge Chironomus sp. are reported. In both mayfly species survival was variable. Complete mortality was observed in salinities with electrical conductivity of 10 mS cm(-1) and higher. Salinities causing chronic mortality in mayflies were measured as 21-day LC50, and ranged from 0.90 to 2.7 mS cm(-1). Growth rates were not significantly different between treatments. In Chironomus, salinity affected the mean number emerging as flying adults as well as the time to emergence. An inverted 'U' shape response was observed for percentage emergence, with the greatest numbers emerging at intermediate salinities (0.65-5.0 mS cm(-1)). No emergence occurred at salinities of 20 mS cm(-1) and higher. Time to emergence was delayed by 15-88% with increased salinity, however the size of emerged adults was the same for all treatments. Growth rates were reduced with increased salinity, showing a slow, steady reduction up to 10 mS cm(-1) then a steep decline between 10 and 15 mS cm(-1). The implications of altered growth rates and changes in developmental times are discussed. This study illustrates the variability in responses to increased salinity, and highlights the need to continue studying sub-lethal and chronic exposures in a range of freshwater invertebrates, in order to predict impacts of salinisation on freshwater biodiversity. PMID:17023596

Hassell, Kathryn L; Kefford, Ben J; Nugegoda, Dayanthi

2006-10-01

301

Identification of novel Sp1 targets involved in proliferation and cancer by functional genomics.  

PubMed

Sp1 is a transcription factor regulating many genes through its DNA binding domain, containing three zinc fingers. We were interested in identifying target genes regulated by Sp1, particularly those involved in proliferation and cancer. Our approach was to treat HeLa cells with a siRNA directed against Sp1 mRNA to decrease the expression of Sp1 and, in turn, the genes activated by this transcription factor. Sp1-siRNA treatment led to a great number of differentially expressed genes as determined by whole genome cDNA microarray analysis. Underexpressed genes were selected since they represent putative genes activated by Sp1 and classified in six Gene Onthology categories, namely proliferation and cancer, mRNA processing, lipid metabolism, glucidic metabolism, transcription and translation. Putative Sp1 binding sites were found in the promoters of the selected genes using the Match™ software. After literature mining, 11 genes were selected for further validation. Underexpression by qRT-PCR was confirmed for the 11 genes plus Sp1 in HeLa cells after Sp1-siRNA treatment. EMSA and ChIP assays were performed to test for binding of Sp1 to the promoters of these genes. We observed binding of Sp1 to the promoters of RAB20, FGF21, IHPK2, ARHGAP18, NPM3, SRSF7, CALM3, PGD and Sp1 itself. Furthermore, the mRNA levels of RAB20, FGF21 and IHPK2 and luciferase activity for these three genes related to proliferation and cancer, were determined after overexpression of Sp1 in HeLa cells, to confirm their regulation by Sp1. Involvement of these three genes in proliferation was validated by gene silencing using polypurine reverse hoogsteen hairpins. PMID:23018034

Oleaga, Carlota; Welten, Sabine; Belloc, Audrey; Solé, Anna; Rodriguez, Laura; Mencia, Núria; Selga, Elisabet; Tapias, Alicia; Noé, Veronique; Ciudad, Carlos J

2012-09-25

302

ARSENIC TRIOXIDE DOWNREGULATES SPECIFICITY PROTEIN (Sp) TRANSCRIPTION FACTORS AND INHIBITS BLADDER CANCER CELL AND TUMOR GROWTH  

PubMed Central

Arsenic trioxide exhibits antiproliferative, antiangiogenic and proapoptotic activity in cancer cells, and many genes associated with these responses are regulated by specificity protein (Sp) transcription factors. Treatment of cancer cells derived from urologic (bladder and prostate) and gastrointestinal (pancreas and colon) tumors with arsenic trioxide demonstrated that these cells exhibited differential responsiveness to the antiproliferative effects of this agent and this paralleled their differential repression of Sp1, Sp3 and Sp4 proteins in the same cell lines. Using arsenic trioxide responsive KU7 and non-responsive 253JB-V bladder cancer cells as models, we show that in KU7 cells, ? 5 ?M arsenic trioxide decreased Sp1, Sp3 and Sp4 and several Sp-dependent genes and responses including cyclin D1, epidermal growth factor receptor, bcl-2, survivin and vascular endothelial growth factor, whereas at concentrations up to 15 ?M, minimal effects were observed in 253JB-V cells. Arsenic trioxide also inhibited tumor growth in athymic mice bearing KU7 cells as xenografts, and expression of Sp1, Sp3 and Sp4 was significantly decreased. Inhibitors of oxidative stress such as glutathione or dithiothreitol protected KU7 cells from arsenic trioxide-induced antiproliferative activity and Sp repression, whereas glutathione depletion sensitized 253JB-V cells to arsenic trioxide. Mechanistic studies suggested that arsenic trioxide-dependent downregulation of Sp and Sp-dependent genes was due to decreased mitochondrial membrane potential and induction of reactive oxygen species, and the role of peroxides in mediating these responses was confirmed using hydrogen peroxide.

Jutooru, Indira; Chadalapaka, Gayathri; Sreevalsan, Sandeep; Lei, Ping; Barhoumi, Rola; Burghardt, Robert; Safe, Stephen

2010-01-01

303

Survey of 150 strains belonging to the Mycobacterium terrae complex and description of Mycobacterium engbaekii sp. nov., Mycobacterium heraklionense sp. nov. and Mycobacterium longobardum sp. nov.  

PubMed

A thorough phenotypic and genotypic analysis of 150 strains belonging to the Mycobacterium terrae complex resulted in the identification of a number of previously unreported sequevars (sqvs) within the species known to belong to the complex. For the species Mycobacterium arupense, three sqvs were detected in the 16S rRNA gene, six sqvs in the hsp65 gene and 15 sqvs in the rpoB gene; in Mycobacterium senuense two sqvs were present in each of the three genetic regions; in Mycobacterium kumamotonense four, two and nine sqvs were found, respectively, and in M. terrae three, four and six sqvs were found, respectively. The inappropriate inclusion of Mycobacterium triviale within the M. terrae complex was confirmed. The limited utility of biochemical tests and of mycolic acid analyses for the differentiation of the members of M. terrae complex was also confirmed. The survey allowed the recognition of three previously undescribed species that were characterized by unique sequences in the 16S rRNA, hsp65 and rpoB genes. Mycobacterium engbaekii sp. nov. (proposed previously 40 years ago but never validly published) was characterized by pink photochromogenic pigmentation and rapid growth; phylogenetically it was related to Mycobacterium hiberniae. The type strain of this species, of which eight strains were investigated, is ATCC 27353(T) (?=?DSM 45694(T)). A cluster of 24 strains was the basis for the description of Mycobacterium heraklionense sp. nov., which has an intermediate growth rate and is unpigmented; nitrate reductase activity is typically strong. Closely related to M. arupense with respect to the 16S rRNA gene, M. heraklionense sp. nov. could be clearly differentiated from the latter species in the other genetic regions investigated. The type strain is NCTC 13432(T) (?=?LMG 24735(T)?=?CECT 7509(T)). Mycobacterium longobardum sp. nov., represented in the study by seven strains, was characterized by a unique phylogenetic location within the M. terrae complex, clearly divergent from any other species. The type strain is DSM 45394(T) (?=?CCUG 58460(T)). PMID:22447702

Tortoli, Enrico; Gitti, Zoe; Klenk, Hans-Peter; Lauria, Stefania; Mannino, Roberta; Mantegani, Paola; Mariottini, Alessandro; Neonakis, Ioannis

2012-03-23

304

Effect of hydrophobic surfactant proteins SP-B and SP-C on phospholipid monolayers. Protein structure studied using 2D IR and beta correlation analysis.  

PubMed Central

We have applied two-dimensional infrared (2D IR) and betanu correlation spectroscopy to in-situ IR spectroscopy of pulmonary surfactant proteins SP-B and SP-C in lipid-protein monolayers at the air-water interface. For both SP-B and SP-C, a statistical windowed autocorrelation method identified two separate surface pressure regions that contained maximum amide I intensity changes: 4-25 mN/m and 25-40 mN/m. For SP-C, 2D IR and betanu correlation analyses of these regions indicated that SP-C adopts a variety of secondary structure conformations, including alpha-helix, beta-sheet, and an intermolecular aggregation of extended beta-sheet structure. The main alpha-helix band split into two peaks at high surface pressures, indicative of two different helix conformations. At low surface pressures, all conformations of the SP-C molecule reacted identically to increasing surface pressure and reoriented in phase with each other. Above 25 mN/m, however, the increasing surface pressure selectively affected the coexisting protein conformations, leading to an independent reorientation of the protein conformations. The asynchronous 2D IR spectrum of SP-B showed the presence of two alpha-helix components, consistent with two separate populations of alpha-helix in SP-B-a hydrophobic fraction associated with the lipid chains and a hydrophilic fraction parallel to the membrane surface. The distribution of correlation intensity between the two alpha-helix cross peaks indicated that the more hydrophobic helix fraction predominates at low surface pressures whereas the more hydrophilic helix fraction predominates at high surface pressures. The different SP-B secondary structures reacted identically to increasing surface pressure, leading to a reorientation of all SP-B subunits in phase with one another.

Shanmukh, Saratchandra; Howell, Phillip; Baatz, John E; Dluhy, Richard A

2002-01-01

305

SP-100 Ground Engineering Systems: Test facility control systems  

SciTech Connect

One of the major objectives of the current phase of the SP-100 program is to demonstrate the performance of a full-scale, prototypic nuclear subsystem of a 100 kWe space nuclear power supply. Such a test is planned at the SP-100 Test Site located at US Department of Energy facilities near Richland, Washington. Considerations involved in selecting a design for the central, integrated facility control system which will control and monitor the performance of the nuclear subsystem and supporting facility systems are discussed. These considerations have been translated into the design of a modern, commerically available, microprocessor based control and monitoring system, using color graphics techniques for the operator interface.

Carlson, W.F.; Henshall, J.B.; Hampsten, K.L.

1988-01-01

306

SP-100 system thaw and start-up strategies  

NASA Astrophysics Data System (ADS)

The authors review several strategies that have been considered during calendar year 1990 for SP-100 system start-up and thaw, and provide results of screening analyses. Screening studies have identified three concepts which are capable of thawing the SP-100 system: (1) a heat pipe approach in which reactor-heated heat pipes are routed to equipment enclosed within a thaw cavity; (2) a bleed-tube approach in which perforated tubes routed through ducting and components achieve thaw progressively as the result of successively thawing bleed-holes; and (3) a NaK traceline approach in which reactor-heated NaK is routed along ducting and components and achieves lithium thaw by both radiative and conductive coupling methods. Key issues have been identified in connection with pump start-up, reactor and power converter transient behavior, and hydraulic circuit stability. Pump start-up stability is accomplished by a linked-pump hydraulic arrangement.

Kirpich, A.; Choe, H.

307

Coagulation-flocculation of marine Chlorella sp. for biodiesel production.  

PubMed

Harvesting of marine Chlorella sp. by autoflocculation and flocculation by addition of coagulant with pH adjustment was investigated in this study. Autoflocculation provided low efficiency. Response surface methodology was employed to optimize the coagulant dosage and pH for flocculation. Aluminium sulfate and ferric chloride were investigated coagulants. The empirical models from RSM are in a good agreement with the experimental results. The optimum flocculation was achieved at ferric chloride dosage 143mg/L, pH 8.1 and settling time 40min. Biomass concentration also presented the significant effect on harvesting efficiency. Lipid extracted from marine Chlorella sp. cultivated in urea fertilizer medium with hexane as a solvent is suitable to produce biodiesel according to it contains high proportion of saturated fatty acids. The crude lipid should be purified to remove some impurities before making biodiesel. As the free fatty acid content was higher than 1% a two-step biodiesel production is recommended. PMID:24012844

Sanyano, Naruetsawan; Chetpattananondh, Pakamas; Chongkhong, Sininart

2013-08-22

308

Parabacteroides chinchillae sp. nov., isolated from chinchilla (Chincilla lanigera) faeces.  

PubMed

Strains of Gram-stain-negative, anaerobic, rod-shaped bacteria were isolated from chinchilla (Chinchilla lanigera) faeces, and strain ST166(T) was investigated taxonomically. Phylogenetic analyses of 16S rRNA gene sequences revealed that strain ST166(T) belonged to the genus Parabacteroides. Strain ST166(T) formed a distinct line of descent, and the highest sequence similarity to ST166(T) was found with Parabacteroides merdae JCM 9497(T) (95.6%) and Parabacteroides johnsonii JCM 13406(T) (95.0%). Analysis of hsp60 gene sequences also supported these relationships. Based on the phenotypic and phylogenetic characteristics, the novel species Parabacteroides chinchillae sp. nov. is proposed. The type strain of P. chinchillae sp. nov. is ST166(T) (?=?JCM 17104(T)?=CCUG 62154(T)). PMID:23563230

Kitahara, Maki; Sakamoto, Mitsuo; Tsuchida, Sayaka; Kawasumi, Koh; Amao, Hiromi; Benno, Yoshimi; Ohkuma, Moriya

2013-04-05

309

Nickel-inducible lysis system in Synechocystis sp. PCC 6803.  

PubMed

We designed and constructed a controllable inducing lysis system in Synechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (i) directly using the phage lysis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii) combining lysis genes from different phages. Significant autolysis was induced in the Synechocystis sp. PCC 6803 cells with this system by the addition of NiSO(4). Our inducible cyanobacterial lysing system eliminates the need for mechanical or chemical cell breakage and could facilitate recovery of biofuel from cyanobacteria. PMID:19995962

Liu, Xinyao; Curtiss, Roy

2009-12-07

310

Complete genome sequence of Paenibacillus sp. strain JDR-2  

PubMed Central

Paenibacillus sp. strain JDR-2, an aggressively xylanolytic bacterium isolated from sweetgum (Liquidambar styraciflua) wood, is able to efficiently depolymerize, assimilate and metabolize 4-O-methylglucuronoxylan, the predominant structural component of hardwood hemicelluloses. A basis for this capability was first supported by the identification of genes and characterization of encoded enzymes and has been further defined by the sequencing and annotation of the complete genome, which we describe. In addition to genes implicated in the utilization of ?-1,4-xylan, genes have also been identified for the utilization of other hemicellulosic polysaccharides. The genome of Paenibacillus sp. JDR-2 contains 7,184,930 bp in a single replicon with 6,288 protein-coding and 122 RNA genes. Uniquely prominent are 874 genes encoding proteins involved in carbohydrate transport and metabolism. The prevalence and organization of these genes support a metabolic potential for bioprocessing of hemicellulose fractions derived from lignocellulosic resources.

Chow, Virginia; Nong, Guang; St. John, Franz J.; Rice, John D.; Dickstein, Ellen; Chertkov, Olga; Bruce, David; Detter, Chris; Brettin, Thomas; Han, James; Woyke, Tanja; Pitluck, Sam; Nolan, Matt; Pati, Amrita; Martin, Joel; Copeland, Alex; Land, Miriam L.; Goodwin, Lynne; Jones, Jeffrey B.; Ingram, Lonnie O.; Shanmugam, Keelnathan T.; Preston, James F.

2012-01-01

311

[Chinius barbazani n. sp. from Thailand (Diptera: Psychodidae)].  

PubMed

A new species of sandfly is described from limestone caves in Thailand. The inclusion of this species in the genus Chinius, which up until now was monospecific, is discussed. It is justified on the basis of characteristics of the head (eyes, pharynx, cibarium, complete interocular suture and length of the mouth pieces), thorax (rounded wings), abdomen (presence of trumpet glands on the tergites 4 and 5 of the male) and genitalia (morphology of the male genitalia and of the spermathecae in the female). Detailed descriptions and drawings are given. The wing of C. barbazani n. sp. lacks of vein R2 in both sexes. This anomaly, regarding to Phlebotominae, is discussed and considered as a probable autapomorphic regression. The differential diagnosis with Chinius junlianensis Leng, 1987, rests on a number of characteristics of the wing venation, antennal formula and the length of the male and female genital ducts, which are five times shorter in C. barbazani n. sp. PMID:16800124

Depaquit, J; Léger, N; Beales, P

2006-06-01

312

Nickel-inducible lysis system in Synechocystis sp. PCC 6803  

PubMed Central

We designed and constructed a controllable inducing lysis system in Synechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (i) directly using the phage lysis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii) combining lysis genes from different phages. Significant autolysis was induced in the Synechocystis sp. PCC 6803 cells with this system by the addition of NiSO4. Our inducible cyanobacterial lysing system eliminates the need for mechanical or chemical cell breakage and could facilitate recovery of biofuel from cyanobacteria.

Liu, Xinyao; Curtiss, Roy

2009-01-01

313

SP-100 Ground Engineering Systems: Test facility control systems  

NASA Astrophysics Data System (ADS)

One of the major objectives of the current phase of the SP-100 program is to demonstrate the performance of a full-scale, prototypic nuclear subsystem of a 100 kWe space nuclear power supply. Such a test is planned at the SP-100 Test Site located at U.S. Department of Energy facilities near Richland, Washington. Considerations involved in selecting a design for the central, integrated facility control system which will control and monitor the performance of the nuclear subsystem and supporting facility systems are discussed. These considerations have been translated into the design of a modern, commercially available, microprocessor based control and monitoring system, using color graphics techniques for the operator interface.

Carlson, W. F.; Henshall, J. B.; Hampsten, K. L.

1988-01-01

314

Complete genome sequence of Paenibacillus sp. strain JDR-2  

SciTech Connect

Paenibacillus sp. strain JDR-2, an aggressively xylanolytic bacterium isolated from sweetgum (Liquidambar styraciflua) wood, is able to efficiently depolymerize, assimilate and metabolize 4-O-methylglucuronoxylan, the predominant structural component of hardwood hemicelluloses. A basis for this capability was first supported by the identification of genes and characterization of encoded enzymes and has been further defined by the sequencing and annotation of the complete genome, which we describe. In addition to genes implicated in the utilization of -1,4-xylan, genes have also been identified for the utilization of other hemicellulosic polysaccharides. The genome of Paenibacillus sp. JDR-2 contains 7,184,930 bp in a single replicon with 6,288 protein-coding and 122 RNA genes. Uniquely prominent are 874 genes encoding proteins involved in carbohydrate transport and metabolism. The prevalence and organization of these genes support a metabolic potential for bioprocessing of hemicellulose fractions derived from lignocellulosic resources.

Chow, Virginia [University of Florida; Nong, Guang [University of Florida; St. John, Franz J. [US Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA; Dickstein, Ellen [University of Florida; Chertkov, Olga [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Brettin, Thomas S [ORNL; Han, James [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Martin, Joel [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Jones, Jeffrey B. [University of Florida; Ingram, Lonnie O. [University of Florida; Shanmugam, Keelnathan T. [University of Florida; Preston, James F. [University of Florida

2012-01-01

315

Complete genome sequence of Thioalkalivibrio sp. K90mix  

Microsoft Academic Search

Thioalkalivibrio sp. K90mix is an obligately chemolithoautotrophic, natronophilic sulfur-oxidizing bacterium (SOxB) belonging to the family Ectothiorhodospiraceae within the Gammaproteobacteria. The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based of its extreme potassium carbonate tolerance as an enrichment method. Here we report the complete genome sequence of

G. Muyzer; D. Y. Sorokin; K. Mavromatis; A. Lapidus; B. Foster; H. Sun; N. Ivanova; A. Pati; P. D’Haeseleer; T. Woyke; N. C. Kyrpides

2011-01-01

316

Seladonia (Pachyceble) henanensis sp. n. (Hymenoptera, Halictidae) from China  

PubMed Central

Abstract Seladonia (Pachyceble) henanensis sp. n., is described from Henan Province, the eastern-central part of China. This species is separated from its allied species by a combination of the following morphological characters: head broad in female, inner hind tibial spur of female with 7–8 slender teeth, T1 basolaterally with appressed hair tuft in both sexes, and genitalia with long and large lower gonostylus in male. Important taxonomic characters are illustrated with photographs, scanning electron micrographs, and line drawings.

Murao, Ryuki; Tadauchi, Osamu; Huan-li, Xu

2013-01-01

317

Characterization of isofenphos hydrolases from Arthrobacter sp. strain B5  

Microsoft Academic Search

Two organophosphorus compound hydrolases constitutively expressed in Arthrobacter sp. strain B-5 that was capable of degrading various organophosphorus insecticides were purified from the cells' cytosol. The enzymes were similarly composed of a single subunit with a molecular weight of 45,000 Da, but had different isoelectric points of 3.6 and 3.9. In addition, the hydrolases were found to possess identical N-terminal

Kazufumi Ohshiro; Tsuyoshi Ono; Tsutomu Hoshino; Takeo Uchiyama

1997-01-01

318

Biological control of alligatorweed ( Alternanthera philoxeroides ) with a Fusarium sp  

Microsoft Academic Search

Alligatorweed (Alternanthera philoxeroidesG.) has become a serious weed in different crops in China. A fungal pathogen was found in Chongqing and Sichuan Provinces and was identified as a species in the Fusarium genus. The fungus produced macroconidia and chlamydospores abundantly on potato sucrose agar (PSA) plates. The bestconidial production and germination and colonygrowth of Fusarium sp. were at 23–31°C and

W. Z. Tan; Q. J. Li; L. Qing

2002-01-01

319

Mercury bioaccumulation and simultaneous nanoparticle synthesis by Enterobacter sp. cells  

Microsoft Academic Search

A mercury resistant strain of Enterobacter sp. is reported. The strain exhibited a novel property of mercury bioaccumulation with simultaneous synthesis of mercury nanoparticles. The culture conditions viz. pH 8.0 and lower concentration of mercury promotes synthesis of uniform sized 2–5nm, spherical and monodispersed intracellular mercury nanoparticles. The remediated mercury trapped in the form of nanoparticles is unable to vaporize

Arvind Sinha; Sunil K. Khare

2011-01-01

320

Screening and productivity of penicillin antibiotic from Penicillium sp.  

PubMed

This paper highlights the antagonism effect of Penicillium isolates, which were screened against the test organisms such as Staphylococcus aureus, E. coli and Penicillium sp. Penicillium notatum and Penicillium chrysogenum isolates were used for penicillin biosynthesis. The antibacterial activities of fermented crude penicillin extract were assayed by disc diffusion method. Maximum antibacterial activity was observed in Gram positive organisms (Staphylococcus aureus) when compared with Gram negative organisms. The isolated Penicillium chrysogenum can be used for large-scale penicillin antibiotic production. PMID:21117415

Sivakumari, V; Dhinakaran, J; Rajendran, A

2009-10-01

321

STREPTOMYCES NODOSUS SP. N., THE AMPHOTERICIN-PRODUCING ORGANISM  

PubMed Central

Trejo, William (Squibb Institute for Medical Research, New Brunswick, N.J.) and Ralph E. Bennett. Streptomyces nodosus sp. n., the amphotericin-producing organism. J. Bacteriol. 85:436–439. 1963.—Streptomyces nodosus, the amphotericin-producing organism, is described as a new species in conformity with the rules of nomenclature as applied to streptomycetes. The relationship between S. nodosus and S. rutgersensis is discussed, and the basis for separation of the species is presented. Images

Trejo, William H.; Bennett, R. E.

1963-01-01

322

Steroid biotransformation by different strains of Micrococcus sp  

Microsoft Academic Search

A strain ofMicrococcus sp. was isolated for its capability of side chain degradation of cholesterol. This strain was characterized and identified\\u000a asMicrococcus roseus. It was found to be the best strain for the production of androsta-1,4-diene-3, 17-dione and androst-4-ene-3, 17-dione compared\\u000a with otherMicrococcus strains.

N. Dogra; G. N. Qazi

2001-01-01

323

Notched tensile strength of SP700 laser welds  

Microsoft Academic Search

Notched tensile tests were performed to evaluate the influence of post-weld heat treatments (PWHTs) on the notched tensile strength (NTS) of two ?+? titanium alloy welds. The results indicated that SP-700 laser welds were notch brittle unless a high PWHT temperature, e.g. 760 °C, was applied. The lowest NTS was associated with the peak-aged weld, which was aged at 482 °C for

L. W. Tsay; Y. S. Ding; W. C. Chung; C. Chen

2008-01-01

324

Anthracnose of Sansevieria trifasciata caused by Colletotrichum sansevieriae sp. nov  

Microsoft Academic Search

A Colletotrichum sp. was isolated from water-soaked lesions on sansevieria (Sansevieria trifasciata Prain cv. Laurentii) in Japan. Classifying the species only from the morphology of the fungus was difficult; therefore, host\\u000a range was tested and the ribosomal DNA ITS2 region was phylogenetically analyzed. The fungus was pathogenic only on sansevieria\\u000a among 20 test plants belonging to 11 families. In a

Masayuki Nakamura; Masahiro Ohzono; Hisashi Iwai; Kei Arai

2006-01-01

325

Homalomena propinqua (Araceae) is Pollinated by Parastasia sp. (Scarabaeidae)  

NSDL National Science Digital Library

Beetles are important pollinators in a lowland dipterocarp forest in Southeast Asia. Most of canopy and emergent dipterocarps are pollinated by beetles feeding on floral tissues. Some understory trees and forest floor herbs are pollinated by different types of beetles. Homalomena propinqua (Araceae) is pollinated by two specias of beetles. One of them, Parastasia sp. (Scarabaeidae), stays inside the spathe chamber and feeds on staminodes.

2004-03-09

326

Physical properties of the DNA of bacteriophage SP50  

Microsoft Academic Search

The following properties of the DNA of B. subtilis phage SP50 were established: Molecular weight (in Daltons) 102×106 (sedimentation velocity) 97×106 (viscosity) 97×106 (contour lengths of electron micrographs) Base Composition (in % GC) 41.7 (chemical analysis) 44 (melting point) 44 (buoyant density) No unusual bases were observed. The complementary strands of the DNA can be separated. The phage DNA has

N. Biswal; A. K. Kleinschmidt; H. C. Spatz; T. A. Trautner

1967-01-01

327

Cytotoxic petrosiacetylenes from the marine sponge Petrosia sp.  

PubMed

A novel petrosiacetylene analog (petrosiacetylene E) has been isolated from the Korean marine sponge Petrosia sp., along with petrosiacetylene A, B and C. Their structures were elucidated on the basis of spectroscopic methods and the stereochemistry of the new compound was determined by using the modified Mosher's method. Petrosiacetylene E showed higher cytotoxicity against five human cancer cell lines than petrosiacetylene A and B, presumably due to the additional hydroxy group located at C-16. PMID:23077003

Lee, Yeon-Ju; Yoo, Su-Jung; Kang, Jong Soon; Yun, Jieun; Shin, Hee Jae; Lee, Jong Seok; Lee, Hyi-Seung

2012-10-18

328

Phyllosticta ophiopogonis sp. nov. from Ophiopogon japonicus (Liliaceae)  

PubMed Central

A leaf spotting disease of an ornamental variety of Ophiopogon japonicus was discovered at several locations in northern Thailand. In all cases a species of Phyllosticta was associated with the lesions. Phyllosticta ophiopogonis sp. nov. is distinguished from Phyllosticta species from Liliaceae in conidia size, mucilaginous sheath and appendage thus the species is introduced as new in this paper. The new species which causes unsightly lesions on this ornamental plant is described, illustrated and compared with other similar Phyllosticta species.

Wikee, S.; Wulandari, N.F.; McKenzie, E.H.C.; Hyde, K.D.

2011-01-01

329

Sesterterpenoids Isolated from a Northeastern Pacific Phorbas sp.  

PubMed

Four new sesterterpenoids, ansellone B (4), phorbadione (5), secoepoxyansellone A (6), and alotaketal C (7), have been isolated from specimens of the sponge Phorbas sp. collected in British Columbia. Ansellone B (4) has an unprecedented heterocyclic skeleton featuring an oxocane ring, and secoepoxyansellone A (6) is the first example of the degraded "secoansellane" sesterterpenoid carbon skeleton. Alotaketal C (7) is an activator of cAMP signaling in HEK cells. PMID:23941043

Daoust, Julie; Chen, Min; Wang, Meng; Williams, David E; Chavez, Miguel Angel Garcia; Wang, Yan Alexander; Merchant, Catherine E; Fontana, Angelo; Kieffer, Timothy J; Andersen, Raymond J

2013-08-20

330

Characterization of cadmium removal by Rhodotorula sp. Y11  

Microsoft Academic Search

Experiments were conducted studying the removal of Cd2+ from water via biosorption using Rhodotorula sp. Y11. The effects of temperature and initial pH of the solution on biosorption were studied. Caustic and heat treatments showed different influences on the biosorption capacity, and the highest metal uptake value (19.38 mg g?1) was obtained by boiling treated yeast cells. The presence of competing

Zhijian Li; Hongli Yuan

2006-01-01

331

Comparison of 128-SP-QAM with PM-16-QAM.  

PubMed

In this paper we investigate an interesting modulation format for fiber optic communications, set-partitioning 128 polarization-multiplexed 16-QAM (128-SP-QAM), which consists of the symbols with even parity from the symbol alphabet of polarization-multiplexed 16-QAM (PM-16-QAM). We compare 128-SP-QAM and PM-16-QAM using numerical simulations in long-haul transmission scenarios at bit rates of 112 Gbit/s and 224 Gbit/s, and at the same symbol rates (14 and 28 Gbaud). The transmission link is made up of standard single-mode fiber with 60, 80 or 100 km amplifier spacing and both single channel and WDM transmission (25- and 50 GHz-spaced) is investigated. The results show that 128-SP-QAM achieves more than 40% increase in transmission reach compared to PM-16-QAM at the same data rate for all cases studied for a bit error rate of 10?³. In addition, we find that in single channel transmission there is, as expected, an advantage in terms of transmission distance when using a data rate of 112 Gbit/s as compared to 224 Gbit/s. However, when comparing the two different WDM systems with the same aggregate data rates, the reach is similar due to the smaller impact of nonlinear crosstalk between the WDM channels in the systems with 50 GHz spacing. We also discuss decoding and phase estimation of 128-SP-QAM and implement differential coding, which avoids error bursts due to cycle slips in the phase estimation. Simulations including laser phase noise show that the phase noise tolerance is similar for the two formats, with 0.5 dB OSNR penalty compared to the case with zero phase noise for a laser linewidth to symbol rate ratio of 10??. PMID:22513547

Sjödin, Martin; Johannisson, Pontus; Li, Jianqiang; Agrell, Erik; Andrekson, Peter A; Karlsson, Magnus

2012-04-01

332

Culturable actinobacteria isolated from marine sponge Iotrochota sp  

Microsoft Academic Search

The study describes the diversity of actinobacteria isolated from the marine sponge Iotrochota sp. collected in the South China Sea. Species and natural product diversity of isolates were analyzed, including screening\\u000a for genes encoding polyketide synthases (PKS) and nonribosomal peptide synthetase (NRPS), and 16S rRNA gene restriction fragment\\u000a length polymorphism (RFLP). PKS and NRPS sequences were detected in more than

Shumei Jiang; Xiang Li; Long Zhang; Wei Sun; Shikun Dai; Lianwu Xie; Yonghong Liu; Kyung Jin Lee

2008-01-01

333

Simultaneous nitrification and denitrification by diverse Diaphorobacter sp  

Microsoft Academic Search

Eight bacterial isolates closely related to Diaphorobacter sp. were isolated from activated biomass surviving on wastewater laden with dyes and nitro-substituted chemicals and were\\u000a identified by 16S rDNA sequence analysis. The isolates showed sequence similarity of 99–100% to other Diaphorobacter strains such as ZY 2006b, F2, NA5, PCA039, D. nitroreducens KSP4, and KSP3 and 98–99% sequence homology to D. nitroreducens

Anshuman A. Khardenavis; Atya Kapley; Hemant J. Purohit

2007-01-01

334

Hypoxia and sulphide influence gamete production in Ulva sp  

Microsoft Academic Search

Gamete production after exposure to hypoxia or sulphide was studied in the marine macroalga Ulva sp. collected in the Sacca di Goro, Italy. Experiments were carried out on discs (12mm diameter) of thalli cultured in artificial sea water in laboratory at 20±1°C, 152?molm?2s?1, 16h photoperiod and 30‰ salinity. Dehydration of thallus was used as inducer of gametogenesis and growth and

Maria Grazia Corradi; Gessica Gorbi; Corrado Zanni

2006-01-01

335

A New Actinomycete Species, Nocardiopsis lucentensis sp. nov  

Microsoft Academic Search

A new species of the genus Nocardiopsis, for which we propose the name Nocardiopsis lucentensis sp. nov. (type strain, strain DSM 44048), was isolated from a salt marsh soil sample near Alicante, Spain. Whole-cell hydrolysates contain the meso isomer of diaminopimelic acid and no characteristic sugar; thus, the cell wall composition is type 111. Menaquinone MK-lO(H,) is the major menaquinone,

A. F. YASSIN; E. A. GALINSKI; A. WOHLFARTH; K.-D. JAHNKE; K. P. SCHAAL; H. G. TRUPER

336

Oil and fat hydrolysis with lipase from Aspergillus sp  

Microsoft Academic Search

Hydrolysis of olive oil, soybean oil, mink fat, lard, palm oil, coconut oil, and a hydrogenated, hardened oil with lipase\\u000a from anAspergillus sp. has been studied. The lipase had high specific activity (60,000 U\\/g) and did not show any positional specificity. The\\u000a lipase proved to be a more effective catalyst than Lipolase fromA. oryzae, with an optimal activity at 37°C

X. Fu; X. Zhu; K. Gao; J. Duan

1995-01-01

337

Differential Equations for Singular Vectors of sp(2n)  

Microsoft Academic Search

Given a weight ? of sp(2n), we derive a system of variable-coefficient second-order linear partial differential equations that determines the singular vectors in the corresponding Verma module. Moreover, we find a family of exact solutions of the system in a certain space of power series. The polynomial solutions correspond to the singular vectors in the Verma module. In particular, we

Xiaoping Xu

2005-01-01

338

Degradation pathways of cyclic alkanes in Rhodococcus sp. NDKK48  

Microsoft Academic Search

The degradation pathways for cyclic alkanes ( c-alkanes) in Rhodococcus sp. NDKK48 were investigated. Strain NDKK48 used dodecylcyclohexane as a sole carbon and energy source, and five metabolites in the dodecylcyclohexane degradation pathway were detected by gas-chromatography\\/mass spectra. The metabolites were identified as cyclohexanecarboxylic acid, cyclohexylacetic acid, 1-cyclohexene-1-acetic acid, 4-dodecylcyclohexanol, and 4-dodecylcyclohexanone. The strain degrades dodecylcyclohexane via a ring oxidation

D. Koma; Y. Sakashita; K. Kubota; Y. Fujii; F. Hasumi; S. Y. Chung; M. Kubo

2004-01-01

339

The first protein map of Synechococcus sp. strain PCC 7942  

Microsoft Academic Search

The first protein map was developed of Synechococcus sp. strain PCC 7942, a model organism for studies of photosynthesis, prokaryotic circadian rhythms, cell division, carbon-concentrating\\u000a mechanisms, and adaptive responses to a variety of stresses. The proteome was analyzed by two-dimensional gel electrophoresis\\u000a with subsequent MALDI-TOF mass spectroscopy and database analysis. Of the 140 analyzed protein spots, 110 were successfully\\u000a identified

O. A. Koksharova; Johan Klint; Ulla Rasmussen

2006-01-01

340

Metabolic Engineering of Indene Bioconversion in Rhodococcus sp  

Microsoft Academic Search

We have applied the methodology of metabolic engineering in the investigation of the enzymatic bioreaction network in Rhodococcus sp. that catalyzes the bioconversion of indene to (2R)-indandiol suitable for the synthesis of cis-1-amino-2-indanol, a precursor of the HIV protease inhibitor, Crixivan. A chemostat with a novel indene air delivery system\\u000a was developed to facilitate the study of steady state physiology

Daniel E. Stafford; Kurt S. Yanagimachi; Gregory Stephanopoulos

341

XPS and XANES studies of uranium reduction by Clostridium sp  

Microsoft Academic Search

Speciation of uranium in cultures of Clostridium sp. by X-ray absorption near-edge spectroscopy (XANES) at the National Synchrotron Light Source and by X-ray photoelectron spectroscopy (XPS) showed that U(VI) was reduced to U(IV). In addition to U(IV), a lower oxidation state of uranium, most probably U(III), was detected by XANES in the bacterial cultures. Reduction of uranium occurred only in

Arokiasamy J. Francis; Cleveland J. Dodge; Fulong Lu; Gary P. Halada; Clive R. Clayton

1994-01-01

342

BEHAVIORAL DEVELOPMENT IN WILD BOTTLENOSE DOLPHIN NEWBORNS (TURSIOPS SP.)  

Microsoft Academic Search

Summary Newborn characteristics, patterns of motoric and social behavioural development, and mother-infant relationships in free-ranging and semi-provisioned bottlenose dolphins ( Tur- siops sp.) are examined. Nine newborns were observed for 189 hours over the é rst 10 weeks of life. Newborn infants breathe more often than their mothers, and synchronize their breath- ing and swimming with her soon after birth,

JANET MANN; BARBARA SMUTS

343

Growth of a manganese oxidizing Pseudomonas sp. in continuous culture  

Microsoft Academic Search

Strain S-36, a marine Pseudomonas sp., was grown under manganese limitation in continuous culture. At dilution rates below a maximal growth rate of 0.066 h-1, the rate at which the organism fixed CO2 into macromolecules was equal to the cell carbon production rate. In addition, the total amount of cell carbon or CO2 fixed at steady-state was in proportion to

P. E. Kepkay; K. H. Nealson

1987-01-01

344

Five New Diterpenoids from an Okinawan Soft Coral, Cespitularia sp.  

PubMed Central

Five new diterpenoids 1–5 were isolated from an Okinawan soft coral, Cespitularia sp., together with the known diterpenoid, alcyonolide (6). New diterpenoid structures were elucidated by spectroscopic methods and by comparison of their NMR data with those of related compounds. Alcyonolide (6) was cytotoxic against HCT 116 cells (IC50 5.85 ?M), while these new diterpenoids 1–5 were much less active (IC50 28.2–91.4 ?M).

Roy, Prodip K.; Maarisit, Wilmar; Roy, Michael C.; Taira, Junsei; Ueda, Katsuhiro

2012-01-01

345

Elicitation of Taxus sp. cell cultures for production of taxol  

Microsoft Academic Search

The addition of cell extracts and cultures filtrate of Pencillium minioluteum, Botrytis cinerea, Verticillium dahliae, and Gilocladium deliqucescens on the tenth day after transferring Taxus sp. (RO1-M28) cell suspensions into an induction medium, further improved the production of TaxolTaxol is a registered trademark of Bristol Myers Squibb for paclitaxel. and total taxanes. Arachidonic acid (1mg\\/L) addition at the time of

Veeresham Ciddi; Venkatesh Srinivasan; M. L. Shuler

1995-01-01

346

Promoter recognition by phage SP01-modified RNA polymerase.  

PubMed Central

A modified form of Bacillus subtilis RNA polymerase containing a phage SP01-coded regulatory protein (the gene 28 product) selectively transcribes "middle" genes of the phage genome in vitro. In this paper, we identify a subset of restriction endonuclease fragments of SP01 DNA that promote specific transcription by the phage-modified polymerase. In the absence of nucleoside triphosphates, RNA polymerase containing the gene 28 protein selectively binds to these DNA fragments thereby forming stable binary complexes that can be isolated on nitrocellulose filters. In contrast, unmodified RNA polymerase containing sigma factor selectively binds to and transcribes a subset of phage DNA fragments that contain "early" sequences and that are in large part distinct from the fragments recognized by the phage-modified transcriptase. Our results strongly suggest that phage "early" and "middle" genes are transcribed from distinct promoters and that the RNA polymerase containing the gene 28 protein binds to sites that are located at or near promoters for SP01 "middle" genes. Images

Talkington, C; Pero, J

1978-01-01

347

Description of Rotylenchus graecus n. sp. from Greece (Nematoda: Hoplolaimidae).  

PubMed

Rotylenchus graecus n. sp. is described and illustrated from a bisexual population found in Filippias, Epirus, northwestern Greece, in the rhizosphere of ivy (Hedera helix L.) and giant reed (Arundo donax L.) grown on the edge of irrigation canals. Rotylenchus graecus n. sp. is characterized by a lip region with five annules, stylet length of 29-32 mum, vulva position at 53-65%, female tail pointed-conoid with 15-23 annules, and bursa with regularly annulated margins enveloping tail. The new species differs from all other Rotylenchus species with couoid tails in having a greater number of tail annules. Rotylenchus graecus n. sp. appears closely related to R. acuspicaudatus Van den Berg &Heyns, 1974, but differs from it primarily by the greater number of female tail annules (15-23 vs. 13-16), stylet length (29--32 vs. 25.7-28.3 mum), and the less ventrally protruding bursa, which has smooth posterior margins rather than the finger-like appearance of the posterior bursal annules in R. acuspicaudatus. PMID:19277350

Vovlas, N; Troccoli, A

1996-03-01

348

SP-100 multimegawatt scaleup to meet electric propulsion mission requirements  

NASA Astrophysics Data System (ADS)

The SP-100 nuclear heat source technology, utilizing uranium nitride fuel clad in PWC-11 in a fast reactor with lithium coolant circulated by an electromagnetic pump, is shown to be directly extrapolatable to thermal power levels that meet NASA nuclear electric propulsion requirements using different power conversion techniques. The SP-100 nuclear technology can be applied to missions with NEP (nuclear electric propulsion) requirements as low as tens of kWe to tens of MWe. It is pointed out that the SP-100 heat source has a great advantage of very long lifetime capability, since it utilizes very rugged refractory metal fuel pins and is independent of the power conversion scheme chosen for a given mission. The only moving parts in the nuclear subsystems are the control rods moved to compensate for fuel enrichment degradation due to fission and for power shutdown. Lowest alpha values in the range of interest for potential NASA missions are predicted for the dynamic Rankine and static HYTEC conversion systems.

Newkirk, D. W.; Salamah, S. A.; Stewart, S. L.; Pluta, P. R.

349

Purification and characterization of chitinase from Paenibacillus sp. D1.  

PubMed

A 56.56-kDa extracellular chitinase from Paenibacillus sp. D1 was purified to 52.3-fold by ion exchange chromatography using SP Sepharose. Maximum enzyme activity was recorded at pH 5.0 and 50 °C. MALDI-LC-MS/MS analysis identified the purified enzyme as chitinase with 60% similarity to chitinase Chi55 of Paenibacillus ehimensis. The activation energy (E (a)) for chitin hydrolysis and temperature quotient (Q (10)) at optimum temperature was found to be 19.14 kJ/mol and 1.25, respectively. Determination of kinetic constants k (m), V (max), k (cat), and k (cat)/k (m) and thermodynamic parameters ?H*, ?S*, ?G*, ?G*(E-S), and ?G*(E-T) revealed high affinity of the enzyme for chitin. The enzyme exhibited higher stability in presence of commonly used protectant fungicides Captan, Carbendazim, and Mancozeb compared to control as reflected from the t (1/2) values suggesting its applicability in integrated pest management for control of soil-borne fungal phytopathogens. The order of stability of chitinase in presence of fungicides at 80 °C as revealed from t (1/2) values and thermodynamic parameters E (a(d)) (activation energy for irreversible deactivation), ?H*, ?G*, and ?S* was: Captan > Carbendazim > Mancozeb > control. The present study is the first report on thermodynamic and kinetic characterization of chitinase from Paenibacillus sp. D1. PMID:21049291

Singh, Anil Kumar; Chhatpar, Hari S

2010-10-29

350

Molasses as fermentation substrate for levan production by Halomonas sp.  

PubMed

Levan is a homopolymer of fructose with many outstanding properties like high solubility in oil and water, strong adhesiveness, good biocompatibility, and film-forming ability. However, its industrial use has long been hampered by costly production processes which rely on mesophilic bacteria and plants. Recently, Halomonas sp. AAD6 halophilic bacteria were found to be the only extremophilic species producing levan at high titers in semi-chemical medium containing sucrose, and in this study, pretreated sugar beet molasses and starch molasses were both found to be feasible substitutes for sucrose. Five different pretreatment methods and their combinations were applied to both molasses types. Biomass and levan concentrations reached by the Halomonas sp. AAD6 cells cultivated on 30 g/L of pretreated beet molasses were 6.09 g dry cells/L and 12.4 g/L, respectively. When compared with literature, Halomonas sp. was found to stand out with its exceptionally high levan production yields on available fructose. Molecular characterization and monosaccharide composition studies confirmed levan-type fructan structure of the biopolymers. Rheological properties under different conditions pointed to the typical characteristics of low viscosity and pseudoplastic behaviors of the levan polymers. Moreover, levan polymer produced from molasses showed high biocompatibility and affinity with both cancerous and non-cancerous cell lines. PMID:21161209

Küçüka?ik, Faruk; Kazak, Hande; Güney, Dilvin; Finore, Ilaria; Poli, Annarita; Yenigün, Orhan; Nicolaus, Barbara; Oner, Ebru Toksoy

2010-12-16

351

Two New Species of Pristionchus (Rhabditida: Diplogastridae): P. fissidentatus n. sp. from Nepal and La Réunion Island and P. elegans n. sp. from Japan.  

PubMed

Pristionchus fissidentatus n. sp., isolated from soil in Nepal, and P. elegans n. sp., isolated from Phelotrupes auratus (Coleoptera: Scarabaeidae) in Japan, are described. The two new species are recognized as basal within the genus and thus occupy an important position for macroevolutionary studies that center on the model P. pacificus. Pristionchus fissidentatus n. sp. is distinguished by its unique stegostomatal morphology: in the stenostomatous form, the right subventral ridge has three prominent cusps and the left subventral sector has, in addition to a plate with two cusps, a prominent denticle slightly left of ventral; in the eurystomatous form, the right subventral stegostomatal sector shows both a tooth and a ridge with several cusps. Diagnostic of P. elegans n. sp. is the structure of the stenostomatous cheilostom, which bulges medially and is underlain by a large vacuolated ring. No eurystomatous form has been observed in P. elegans n. sp. Reproductive modes of P. fissidentatus n. sp. and P. elegans n. sp. are hermaphroditic and gonochoristic, respectively. The additional isolation of P. fissidentatus n. sp. from soil and two species of scarab beetle on La Réunion Island in the Indian Ocean suggests a broad geographic range for this species. PMID:23483847

Kanzaki, Natsumi; Ragsdale, Erik J; Herrmann, Matthias; Sommer, Ralf J

2012-03-01

352

Oxidative 1,2-difunctionalization of activated alkenes with benzylic C(sp(3))-H bonds and aryl C(sp(2))-H bonds.  

PubMed

DTBP (di-tert-butyl peroxide) is utilized to mediate oxidative 1,2-difunctionalization of activated alkenes with an aryl C(sp(2))-H bond and a benzylic C(sp(3))-H bond for the synthesis of functionalized oxindoles. This reaction is a new organomediated strategy for alkene difunctionalization facilitated by Lewis acids. PMID:24113153

Zhou, Ming-Bo; Wang, Cheng-Yong; Song, Ren-Jie; Liu, Yu; Wei, Wen-Ting; Li, Jin-Heng

2013-10-24

353

Heat Curing of a Sym Plasmid in a Fast-Growing Rhizobium sp. That Is Able to Nodulate Legumes and the Nonlegume Parasponia sp  

PubMed Central

Genes involved in nodulation of both legumes and the nonlegume Parasponia sp., as well as nitrogenase genes, reside on a large plasmid in a fast-growing Rhizobium sp. from Lablab purpureus. This plasmid can be cured by incubation at elevated temperatures and can be mobilized by the P1 group plasmid RP1::Tn501. Images

Morrison, Nigel A.; Hau, Cen Y.; Trinick, Michael J.; Shine, John; Rolfe, Barry G.

1983-01-01

354

Heat curing of a sym plasmid in a fast-growing Rhizobium sp. that is able to nodulate legumes and the nonlegume Parasponia sp.  

PubMed

Genes involved in nodulation of both legumes and the nonlegume Parasponia sp., as well as nitrogenase genes, reside on a large plasmid in a fast-growing Rhizobium sp. from Lablab purpureus. This plasmid can be cured by incubation at elevated temperatures and can be mobilized by the P1 group plasmid RP1::Tn501. PMID:6571729

Morrison, N A; Hau, C Y; Trinick, M J; Shine, J; Rolfe, B G

1983-01-01

355

Efficacy of Artesunate + Sulphadoxine-Pyrimethamine (AS + SP) and Amodiaquine + Sulphadoxine-Pyrimethamine (AQ + SP) for Uncomplicated falciparum Malaria in Equatorial Guinea (Central Africa)  

PubMed Central

Objectives. The objectives of the study were (i) to evaluate the efficacy of combination drugs, such as artesunate + sulphadoxine-pyrimethamine (AS?+?SP) and amodiaquine + sulphadoxine-pyripethamine (AQ?+?SP) in treatment of uncomplicated falciparum malaria (ii) to differentiate recrudescence from reinfection by analysing msp-1 and msp-2 genes of Plasmodium falciparum in treatment failure cases. Methods. We carried out an in vivo study in the year 2005 in 206 children between 6 to 59 months age groups. Of the 206, 120 received AQ?+?SP, and 86 received AS?+?SP. A clinical and parasitological followup during 14 days was undertaken. Finger-prick blood sample from each patient was taken on Whatman filter paper (no. 3) on days 0, 7, 14 and also the day when the parasite and symptoms reappeared for PCR analysis. Results. Late treatment failure was observed in 3.5% (4/114) with AQ?+?SP, and 2.5% (2/79) with AS?+?SP. The success rate was 96.5% with AQ?+?SP and 97.5% with AS?+?SP. No deaths and severe reactions were recorded. Out of the 6 treatment failure cases, one was reinfection as observed by PCR analysis of msp-1 and msp-2 genes on day 14. Discussion. Both the combinations found to be efficacious and safe and could be used as a first-line treatment for uncomplicated falciparum malaria in Equatorial Guinea.

Charle, Pilar; Berzosa, Pedro; Descalzo, Miguel Angel; de Lucio, Aida; Raso, Jose; Obono, Jacqueline; Lwanga, Magdalena; Nlang, Natividad; Nchama, Araceli; Mangue, Catalina; Micha, Anastasio; Nsee, Natividad; Mesie, Rosario; Benito, Agustin; Roche, Jesus

2009-01-01

356

A functional comparison of the venom of three Australian jellyfish— Chironex fleckeri, Chiropsalmus sp., and Carybdea xaymacana—on cytosolic Ca 2+, haemolysis and Artemia sp. lethality  

Microsoft Academic Search

Cnidarian venoms produce a wide spectrum of envenoming syndromes in humans ranging from minor local irritation to death. Here, the effects of Chironex fleckeri, Chiropsalmus sp., and Carybdea xaymacana venoms on ventricular myocyte cytosolic Ca2+, haemolysis and Artemia sp. lethality are compared for the first time. All three venoms caused a large, irreversible elevation of cytosolic Ca2+ in myocytes as

Paul M. Bailey; Anthony J. Bakker; Jamie E. Seymour; Jacqueline A. Wilce

2005-01-01

357

Cryptosporidium agni sp.n. from lambs, and Cryptosporidium bovis sp.n. from a calf, with observations on the oocyst  

Microsoft Academic Search

Cryptosporidium agni sp. n. is described from the small intestine, particularly ileum, of lambs, Ovis aries, and C. bovis sp. n. is described from a similar location in a calf Bos taurus. Ultrastructural observations on trophozoites, schizonts and macrogametes indicated close similarity between the morphology of these species and that of C. wrairi. Oocysts undergoing sporogony, enveloped by an oocyst

I. K. Barker; P. L. Carbonell

1974-01-01

358

Babesia bicornis sp. nov. and Theileria bicornis sp. nov.: tick-borne parasites associated with mortality in the black rhinoceros (Diceros bicornis).  

PubMed

A novel Babesia species, designated Babesia bicornis sp. nov., was identified in three black rhinoceroses (Diceros bicornis) that died in wildlife areas in Tanzania and South Africa. Screening of black rhinoceroses in South Africa revealed, in addition to B. bicornis, a second parasite, designated Theileria bicornis sp. nov. PMID:12734294

Nijhof, Ard M; Penzhorn, Banie L; Lynen, Godelieve; Mollel, Johnson O; Morkel, Pete; Bekker, Cornelis P J; Jongejan, Frans

2003-05-01

359

Babesia bicornis sp. nov. and Theileria bicornis sp. nov.: Tick-Borne Parasites Associated with Mortality in the Black Rhinoceros (Diceros bicornis)  

PubMed Central

A novel Babesia species, designated Babesia bicornis sp. nov., was identified in three black rhinoceroses (Diceros bicornis) that died in wildlife areas in Tanzania and South Africa. Screening of black rhinoceroses in South Africa revealed, in addition to B. bicornis, a second parasite, designated Theileria bicornis sp. nov.

Nijhof, Ard M.; Penzhorn, Banie L.; Lynen, Godelieve; Mollel, Johnson O.; Morkel, Pete; Bekker, Cornelis P. J.; Jongejan, Frans

2003-01-01

360

Two New Species of Pristionchus (Rhabditida: Diplogastridae): P. fissidentatus n. sp. from Nepal and La R?union Island and P. elegans n. sp. from Japan  

PubMed Central

Pristionchus fissidentatus n. sp., isolated from soil in Nepal, and P. elegans n. sp., isolated from Phelotrupes auratus (Coleoptera: Scarabaeidae) in Japan, are described. The two new species are recognized as basal within the genus and thus occupy an important position for macroevolutionary studies that center on the model P. pacificus. Pristionchus fissidentatus n. sp. is distinguished by its unique stegostomatal morphology: in the stenostomatous form, the right subventral ridge has three prominent cusps and the left subventral sector has, in addition to a plate with two cusps, a prominent denticle slightly left of ventral; in the eurystomatous form, the right subventral stegostomatal sector shows both a tooth and a ridge with several cusps. Diagnostic of P. elegans n. sp. is the structure of the stenostomatous cheilostom, which bulges medially and is underlain by a large vacuolated ring. No eurystomatous form has been observed in P. elegans n. sp. Reproductive modes of P. fissidentatus n. sp. and P. elegans n. sp. are hermaphroditic and gonochoristic, respectively. The additional isolation of P. fissidentatus n. sp. from soil and two species of scarab beetle on La Réunion Island in the Indian Ocean suggests a broad geographic range for this species.

Kanzaki, Natsumi; Ragsdale, Erik J.; Herrmann, Matthias; Sommer, Ralf J.

2012-01-01

361

Description of Thermococcus kodakaraensis sp. nov., a well studied hyperthermophilic archaeon previously reported as Pyrococcus sp. KOD1  

PubMed Central

A hyperthermophilic archaeal strain, KOD1, isolated from a solfatara on Kodakara Island, Japan, has previously been reported as Pyrococcus sp. KOD1. However, a detailed phylogenetic tree, made possible by the recent accumulation of 16S rRNA sequences of various species in the order Thermococcales, indicated that strain KOD1 is a member of the genus Thermococcus. We performed DNA–DNA hybridization tests against species that displayed high similarity in terms of 16S ribosomal DNA sequences, including Thermococcus peptonophilus and Thermococcus stetteri. Hybridization results and differences in growth characteristics and substrate utilization differentiated strain KOD1 from T. peptonophilus and T. stetteri at the species level. Our results indicate that strain KOD1 represents a new species of Thermococcus, which we designate as Thermococcus kodakaraensis KOD1 sp. nov.

Atomi, Haruyuki; Fukui, Toshiaki; Kanai, Tamotsu; Morikawa, Masaaki; Imanaka, Tadayuki

2004-01-01

362

Komagataella populi sp. nov. and Komagataella ulmi sp. nov., two new methanol assimilating yeasts from exudates of deciduous trees.  

PubMed

Two new species of the methanol assimilating ascosporic yeast genus Komagataella are described. Komagataella populi sp. nov. (NRRL YB-455, CBS 12362, type strain, MycoBank accession number = 564110) was isolated from an exudate on a cottonwood tree (Populus deltoides), Peoria, Illinois, USA, and Komagataella ulmi sp. nov. (NRRL YB-407, CBS 12361, type strain, MycoBank accession number = 564111) was isolated from the exudate on an elm tree (Ulmus americana), also growing in Peoria, Illinois. The species were resolved from divergence in gene sequences for domains D1/D2 LSU rRNA, ITS1-5.8S-ITS2, mitochondrial small subunit rRNA, RNA polymerase subunit 1 and translation elongation factor-1?. Species of Komagataella assimilate few carbon compounds and are unlikely to be resolved from differences in standard growth and fermentation tests. For this reason, separation of species is dependent on gene sequence analysis. PMID:22302468

Kurtzman, Cletus P

2012-02-01

363

Two novel ascomycetous yeast species, Wickerhamomyces scolytoplatypi sp. nov. and Cyberlindnera xylebori sp. nov., isolated from ambrosia beetle galleries.  

PubMed

Thirteen strains of yeasts were isolated from ambrosia beetle galleries at several sites in Japan. Based on the morphological and biochemical characteristics and phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene of the yeasts, 10 strains were shown to represent a novel species of the genus Wickerhamomyces, described as Wickerhamomyces scolytoplatypi sp. nov. (type strain NBRC 11029(T) = CBS 12186(T)), and were closely related to Wickerhamomyces hampshirensis. The three other strains represented a novel species of the genus Cyberlindnera, described as Cyberlindnera xylebori sp. nov. (type strain NBRC 11048(T) = CBS 12187(T)), and were closely related to Cyberlindnera euphorbiiphila. It is suggested that these species are associated with ambrosia beetles and we consider ambrosia beetle galleries as good sources of novel yeasts. PMID:23667144

Ninomiya, Shinya; Mikata, Kozaburo; Kajimura, Hisashi; Kawasaki, Hiroko

2013-05-10

364

Identification of Arthrobacter oxydans, Arthrobacter luteolus sp. nov., and Arthrobacter albus sp. nov., Isolated from Human Clinical Specimens  

PubMed Central

Five Arthrobacter isolates from clinical specimens were studied by phenotypic, chemotaxonomic, and genetic characterization. Two strains had characteristics consistent with those of Arthrobacter oxydans. One strain was related to A. citreus; however, DNA-DNA hybridization and phenotypic characteristics indicated that this strain belongs to a new species, for which the name Arthrobacter luteolus sp. nov. is proposed. Two strains were closely related to A. cumminsii by 16S rRNA gene sequencing, but DNA-DNA hybridization, peptidoglycan type, and some phenotypic features indicated that they should be assigned to a new species, for which the name Arthrobacter albus sp. nov. is proposed. The type strain of A. luteolus is CF25 (DSM 13067). The type strain of A. albus is CF43 (DSM 13068).

Wauters, Georges; Charlier, Jacqueline; Janssens, Michele; Delmee, Michel

2000-01-01

365

Enterococcus ureilyticus sp. nov. and Enterococcus rotai sp. nov., two urease-producing enterococci from the environment.  

PubMed

A set of 25 urease-producing, yellow-pigmented enterococci was isolated from environmental sources. Phenotypic classification divided the isolates into two phena. Both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all members of the genus Enterococcus with validly published names and placed them in the Enterococcus faecalis species group. DNA-DNA hybridization experiments, pheS and rpoA sequencing and whole-cell protein electrophoresis provided conclusive evidence for the classification of each phenon as a novel species of the genus Enterococcus, for which the names Enterococcus ureilyticus sp. nov. (type strain CCM 4629(T) ?=?LMG 26676(T) ?=?CCUG 48799(T)), inhabiting water and plants, and Enterococcus rotai sp. nov. (type strain CCM 4630(T) ?=?LMG 26678(T) ?=?CCUG 61593(T)), inhabiting water, insects (mosquitoes) and plants, are proposed. PMID:22523160

Sedlá?ek, Ivo; Holochová, Pavla; Mašla?ová, Ivana; Kosina, Marcel; Spröer, Cathrin; Bryndová, Hana; Vandamme, Peter; Rudolf, Ivo; Hubálek, Zdenek; Švec, Pavel

2012-04-20

366

Síndrome antifosfolípido secundario a lupus eritematoso sistémico en un hombre  

Microsoft Academic Search

Resumen Paciente de sexo masculino de 23 años con sín- tomas de una semana de evolución de cambios en el comportamiento, y seis meses de pérdida de peso, eritema malar y palpebral, úlceras orales, se le detecta anticuerpos antinucleares (ANAS) positi- vos, planteándose como diagnóstico, lupus erite- matoso sistémico (LES). El paciente permanece controlado durante tres años; al cabo de

Franco J. Vallejo; Luis Fernando Medina; Claudia Juliana Díaz

367

Two viable three-dimensional carbon semiconductors with an entirely sp2 configuration.  

PubMed

Using the first-principles method, we investigate the energetic stability, dynamic stability and electronic properties of two viable three-dimensional (3D) carbon semiconductors with an entirely sp(2) configuration, sp(2)-diamond and cubic-graphite. Both allotropes are more stable than the previously proposed K4-carbon and T-carbon and were confirmed to be dynamically stable. Interestingly, sp(2)-diamond and cubic-graphite behave as semiconductors, which is contrary to previously proposed all-sp(2) metallic carbons. sp(2)-Diamond is a semiconductor with a direct band gap of 1.66 eV and cubic-graphite is an indirect semiconductor with a band gap of 2.89 eV. Further studies show that both sp(2)-diamond and cubic-graphite possess structural all-sp(2) configurations but not electronic sp(2) hybridizations. The very low densities and entirely sp(2) configurations of sp(2)-diamond and cubic-graphite can be potentially applied in hydrogen-storage, photocatalysts and molecular sieves. PMID:23187896

He, Chaoyu; Sun, Lizhong; Zhang, Chunxiao; Zhong, Jianxin

2012-11-28

368

Transcriptional Activation of REST by Sp1 in Huntington's Disease Models  

PubMed Central

In Huntington's disease (HD), mutant huntingtin (mHtt) disrupts the normal transcriptional program of disease neurons by altering the function of several gene expression regulators such as Sp1. REST (Repressor Element-1 Silencing Transcription Factor), a key regulator of neuronal differentiation, is also aberrantly activated in HD by a mechanism that remains unclear. Here, we show that the level of REST mRNA is increased in HD mice and in NG108 cells differentiated into neuronal-like cells and expressing a toxic mHtt fragment. Using luciferase reporter gene assay, we delimited the REST promoter regions essential for mHtt-mediated REST upregulation and found that they contain Sp factor binding sites. We provide evidence that Sp1 and Sp3 bind REST promoter and interplay to fine-tune REST transcription. In undifferentiated NG108 cells, Sp1 and Sp3 have antagonistic effect, Sp1 acting as an activator and Sp3 as a repressor. Upon neuronal differentiation, we show that the amount and ratio of Sp1/Sp3 proteins decline, as does REST expression, and that the transcriptional role of Sp3 shifts toward a weak activator. Therefore, our results provide new molecular information to the transcriptional regulation of REST during neuronal differentiation. Importantly, specific knockdown of Sp1 abolishes REST upregulation in NG108 neuronal-like cells expressing mHtt. Our data together with earlier reports suggest that mHtt triggers a pathogenic cascade involving Sp1 activation, which leads to REST upregulation and repression of neuronal genes.

Ravache, Myriam; Weber, Chantal; Merienne, Karine; Trottier, Yvon

2010-01-01

369

Transcriptional activation of REST by Sp1 in Huntington's disease models.  

PubMed

In Huntington's disease (HD), mutant huntingtin (mHtt) disrupts the normal transcriptional program of disease neurons by altering the function of several gene expression regulators such as Sp1. REST (Repressor Element-1 Silencing Transcription Factor), a key regulator of neuronal differentiation, is also aberrantly activated in HD by a mechanism that remains unclear. Here, we show that the level of REST mRNA is increased in HD mice and in NG108 cells differentiated into neuronal-like cells and expressing a toxic mHtt fragment. Using luciferase reporter gene assay, we delimited the REST promoter regions essential for mHtt-mediated REST upregulation and found that they contain Sp factor binding sites. We provide evidence that Sp1 and Sp3 bind REST promoter and interplay to fine-tune REST transcription. In undifferentiated NG108 cells, Sp1 and Sp3 have antagonistic effect, Sp1 acting as an activator and Sp3 as a repressor. Upon neuronal differentiation, we show that the amount and ratio of Sp1/Sp3 proteins decline, as does REST expression, and that the transcriptional role of Sp3 shifts toward a weak activator. Therefore, our results provide new molecular information to the transcriptional regulation of REST during neuronal differentiation. Importantly, specific knockdown of Sp1 abolishes REST upregulation in NG108 neuronal-like cells expressing mHtt. Our data together with earlier reports suggest that mHtt triggers a pathogenic cascade involving Sp1 activation, which leads to REST upregulation and repression of neuronal genes. PMID:21179468

Ravache, Myriam; Weber, Chantal; Mérienne, Karine; Trottier, Yvon

2010-12-14

370

Lanceimermis palustris n. sp. and Telomermis palustris n. sp. (Nematoda: Mermithidae) parasites of chironomid (Insecta: Diptera) adults from Lake Alice Bog in northern Minnesota.  

PubMed

In the summer of 2003, chironomid imagoes eclosing from Lake Alice Bog in Minnesota yielded mermithid nematodes of 2 new species. Lanceimermis palustris n. sp. is distinguished from the other 14 species of the genus by the subventral mouth position, thinness of the hook-shaped spicule, body index, and structure of the male tail muscles. Telomermis palustris n. sp. is distinguished from the only other species of the genus by the structure of the amphids, body length, maximum body width, length of the esophagus, and the presence of a minute larval terminal horn. Lanceimermis palustris n. sp. hosts were Chironomus maturus Johannsen and T. palustris n. sp. hosts were Tanytarsus mendax Kieffer, Paratanytarsus nr. dissimilis n. sp., and Micropsectra polita (Malloch). In both mermithid species, the percent of mermithid males per host increased with the intensity of the infection. PMID:15715243

Johnson, Arthur A; Kleve, Maurice G

2004-12-01

371

Integrated Bacillus sp. immobilized cell reactor and Synechocystis sp. algal reactor for the treatment of tannery wastewater.  

PubMed

The wastewater discharged from leather industries lack biodegradability due to the presence of xenobiotic compounds. The primary clarification and aerobic treatment in Bacillus sp. immobilized Chemo Autotrophic Activated Carbon Oxidation (CAACO) reactor removed considerable amount of pollution parameters. The residual untreated organics in the wastewater was further treated in algal batch reactor inoculated with Synechocystis sp. Sodium nitrate, K(2)HPO(4), MgSO(4).7H(2)O, NH(4)Cl, CaCl(2)·2H(2)O, FeCl(3) (anhydrous), and thiamine hydrochloride, rice husk based activated carbon (RHAC), immobilization of Bacillus sp. in mesoporous activated carbon, sand filter of dimensions diameter, 6 cm and height, 30 cm; and the CAACO reactor of dimensions diameter, 5.5 cm and height, 30 cm with total volume 720 ml, and working volume of 356 ml. In the present investigation, the CAACO treated tannery wastewater was applied to Synechocystis sp. inoculated algal batch reactor of hydraulic residence time 24 h. The BOD(5), COD, and TOC of treated wastewater from algal batch reactor were 20 ± 7, 167 ± 29, and 78 ± 16 mg/l respectively. The integrated CAACO system and Algal batch reactor was operated for 30 days and they accomplished a cumulative removal of BOD(5),COD, TOC, VFA and sulphide as 98 %, 95 %, 93 %, 86 %, and 100 %, respectively. The biokinetic constants for the growth of algae in the batch reactor were specific growth rate, 0.095(day(-1)) and yield coefficient, 3.15 mg of algal biomass/mg of COD destructed. The degradation of xenobiotic compounds in the algal batch reactor was confirmed through HPLC and FT-IR techniques. The integrated CAACO-Algal reactor system established a credible reduction in pollution parameters in the tannery wastewater. The removal mechanism is mainly due to co-metabolism between algae and bacterial species and the organics were completely metabolized rather than by adsorption. PMID:22528997

Sekaran, G; Karthikeyan, S; Nagalakshmi, C; Mandal, A B

2012-04-16

372

Sulfurospirillum barnesii sp. nov. and Sulfurospirillum arsenophilum sp. nov., new members of the Sulfurospirillum clade of the ?-Proteobacteria  

USGS Publications Warehouse

Two strains of dissimilatory arsenate-reducing vibrio-shaped bacteria are assigned to the genus Sulfurospirillum. These two new species, Sulfurospirillum barnesii strain SES-3(T) and Sulfurospirillum arsenophilum strain MIT-13(T), in addition to Sulfurospirillum sp. SM-5, two strains of Sulfurospirillum deleyianum, and Sulfurospirillum arcachonense, form a distinct clade within the ?? subclass of the Proteobacteria based on 16S rRNA analysis.

Stolz, J. F.; Ellis, D. J.; Blum, J. S.; Ahmann, D.; Lovley, D. R.; Oremland, R. S.

1999-01-01

373

Spermatozoal ultrastructure in four genera of Homolidae (crustacea, decapoda): Exemplified by Homologenus sp., Latreillopsis sp., Homolomannia sibogae and Paromolosis boasi  

NASA Astrophysics Data System (ADS)

The spermatozoa of Homologenus sp., Latreillopsis sp., Homolomannia sibogae and Paromolopsis boasi confirm characteristics of a distinctive homolid spermatozoon previously established for Homola sp., Paromola sp. and Paromola petterdi. Homolid features are (1) moderate anteroposterior depression of the acrosome (ratio of length: width 0.4 0.6) as in lyreidine raninids (0.5), depression being greater in dromiids and dynomenids (both 0.3); (2) the capitate form of the perforatorium, shared with dromiids, dynomenids and lyreidine raninids; (3)the autapomorphic spiked-wheel form of the anterior expansion of the perforatorium; (4) horizontal zonation of the acrosome is possibly a unique synapomorphy of homolids with dromiids and dynomenids, and therefore an autapomorphy of the dromioid-homolid assemblage. In dromiids the posterior zone is proportionately the larger, while in homolids the anterior zone is the larger. The anterior zone is complexly subdivided in dynomenids; (5) the autapomorphic presence of numerous radial arranged extension of the acrosomal operculum into the perforatorium; (6) presence of nuclear arms, a symplesiomorphy of all investigated crabs, but small or questionably sometimes absent in Dromiidae; (7) absence of microtubules from the nuclear arms, as in dromiids, raninids, higher heterotremes and thoracotremes; (8) transient presence of a posterior median process of the nucleus. The process is not seen in dromiids but occurs in anomurans and lower heterotremes; (9) apical perforation of the operculum, also seen, apparently symplesiomorphically, in dromiids, raninids, and lower heterotreme families; (10) absence of an acrosome ray zone, probably homoplasic with absence in raninids; (11) location of most of the cytoplasm, including tortuous membranes and degenerating mitochondria, below the acrosome, also seen in Lyreidus; (12) presence, in at least some species, of centrioles, unknown in dromiids and raninids and variable in occurrence in heterotremes.

Jamieson, B. G. M.; Guinot, D.; Forges, B. Richer De

1993-10-01

374

[Prevalence of Cyclospora sp., Cryptosporidium sp, microsporidia and fecal coliform determination in fresh fruit and vegetables consumed in Costa Rica].  

PubMed

The presence of Cyclospora sp., Cryptosporidium sp. and microsporidia and the levels of fecal coliforms were determined in lettuce, parsley, cilantro, strawberries and blackberries acquired in local agricultural markets of the Central Valley of Costa Rica, in order to establish the possible transmission risk of these microorganisms and other pathogens from the consumption of these raw products. During the second semester of 2001 and the first of 2002, 50 different samples of each product, 25 taken in the dry season and 25 in the rainy season and coming from five different local agricultural markets were evaluated. The fecal coliforms count was done according to the technique recommended by Vanderzant & Splittstoesser. The parasite determination was done using Zielh Nielsen and Weber staining techniques from a sediment obtained through the rinse of the mentioned products, using sterile peptonated water 0.1% and centrifuging at 900 G for 15 min. One hundred per cent of vegetable samples had fecal coliforms and the greatest prevalence was obtained during the rainy season. Although all vegetables presented fecal coliforms in high concentrations, lettuce and cilantro presented statistical difference between rainy and dry season, being greater during the rainy season. Fecal coliforms were not detected in strawberries and blackberries probablydue to its low pH. All products evaluated presented, at least once, Cryptosporidium sp., Cyclospora sp. and microsporidia, showing the risk they represent to Public Health. Cryptosporidium was present in all products but strawberries. Microsporidia was present in all products except blackberries and Cyclospora was only isolated from lettuce during the dry season. These results show the importance of introducing in the country Good Agricultural Practices, especially due to the resistance of Cryptosporidium and Cyclospora to disinfecting agents. PMID:15969268

Calvo, Melvin; Carazo, Melissa; Arias, Maria Laura; Chaves, Carolina; Monge, Rafael; Chinchilla, Misael

2004-12-01

375

Potential inhibitors against Sclerotinia sclerotiorum , produced by the fungus Myrothecium sp. associated with the marine sponge Axinella sp  

Microsoft Academic Search

Sclerotinia sclerotiorum is a worldwide ascomycete fungal plant pathogen, which causes enormous yield losses on major economic crops such as crucifers,\\u000a grain legumes and several other plant families. The objective of this research was to isolate and characterise some bioactive\\u000a products from cultures of fungi associated with the marine sponge Axinella sp. In total, nine fungal isolates were obtained from

Lian Wu Xie; Shu Mei Jiang; Hong Hui Zhu; Wei Sun; Yong Chang Ouyang; Shi Kun Dai; Xiang Li

2008-01-01

376

In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement  

PubMed Central

Background Mice lacking surfactant protein-A (SP-A-/-; knockout; KO) exhibit increased vulnerability to infection and injury. Although many bronchoalveolar lavage (BAL) protein differences between KO and wild-type (WT) are rapidly reversed in KO after infection, their clinical course is still compromised. We studied the impact of SP-A on the alveolar macrophage (AM) proteome under basal conditions. Male SP-A KO mice were SP-A-treated (5 micrograms/mouse) and sacrificed in 6 or 18 hr. The AM proteomes of KO, SP-A-treated KO, and WT mice were studied by 2D-DIGE coupled with MALDI-ToF/ToF and AM actin distribution was examined by phalloidon staining. Results We observed: a) significant differences from KO in WT or exogenous SP-A-treated in 45 of 76 identified proteins (both increases and decreases). These included actin-related/cytoskeletal proteins (involved in motility, phagocytosis, endocytosis), proteins of intracellular signaling, cell differentiation/regulation, regulation of inflammation, protease/chaperone function, and proteins related to Nrf2-mediated oxidative stress response pathway; b) SP-A-induced changes causing the AM proteome of the KO to resemble that of WT; and c) that SP-A treatment altered cell size and F-actin distribution. Conclusions These differences are likely to enhance AM function. The observations show for the first time that acute in vivo SP-A treatment of KO mice, under basal or unstimulated conditions, affects the expression of multiple AM proteins, alters F-actin distribution, and can restore much of the WT phenotype. We postulate that the SP-A-mediated expression profile of the AM places it in a state of "readiness" to successfully conduct its innate immune functions and ensure lung health.

2011-01-01

377

Exposure of Immunocompetent Adult Mice to Pneumocystis carinii f. sp. muris by Cohousing: Growth of P. carinii f. sp. muris and Host Immune Response  

PubMed Central

There has been emerging evidence that immunocompetent hosts can harbor Pneumocystis in their lungs. The purpose of this study was to determine the kinetics of Pneumocystis carinii f. sp. muris infection in adult immunocompetent mice and the host immune response to the organisms. To accomplish this, we exposed adult immunocompetent mice to SCID mice infected with P. carinii f. sp. muris by cohousing. We found that P. carinii f. sp. muris was detectable in the lungs of cohoused immunocompetent mice by PCR by 3 weeks after the beginning of cohousing. At about 4 weeks of cohousing, P. carinii f. sp. muris was readily detectable in the lungs of mice by microscopic techniques. Also at this time, P. carinii f. sp. muris-specific immunoglobulin G was found in the sera of the mice, and CD62low CD4- and CD8-positve T cells accumulated in the lungs. Shortly after this immune response, the P. carinii f. sp. muris organisms were cleared from the lungs. Adult mice cohoused for only 1 week also contained P. carinii f. sp. muris cysts detectable by silver staining at 5 and 6 weeks after the beginning of cohousing. We also found that the P. carinii f. sp. muris organisms grew to greater numbers in the lungs of BALB/c mice than in those of C57BL6 mice. This indicates that immunocompetent hosts develop a mild infection with P. carinii f. sp. muris which resolves in 5 to 6 weeks when there is a detectable immune response to the organism. Once an acquired immune response was initiated, the P. carinii f. sp. muris organisms were quickly eliminated without clinical signs of disease.

An, Chun Li; Gigliotti, Francis; Harmsen, Allen G.

2003-01-01

378

Exposure of immunocompetent adult mice to Pneumocystis carinii f. sp. muris by cohousing: growth of P. carinii f. sp. muris and host immune response.  

PubMed

There has been emerging evidence that immunocompetent hosts can harbor Pneumocystis in their lungs. The purpose of this study was to determine the kinetics of Pneumocystis carinii f. sp. muris infection in adult immunocompetent mice and the host immune response to the organisms. To accomplish this, we exposed adult immunocompetent mice to SCID mice infected with P. carinii f. sp. muris by cohousing. We found that P. carinii f. sp. muris was detectable in the lungs of cohoused immunocompetent mice by PCR by 3 weeks after the beginning of cohousing. At about 4 weeks of cohousing, P. carinii f. sp. muris was readily detectable in the lungs of mice by microscopic techniques. Also at this time, P. carinii f. sp. muris-specific immunoglobulin G was found in the sera of the mice, and CD62(low) CD4- and CD8-positve T cells accumulated in the lungs. Shortly after this immune response, the P. carinii f. sp. muris organisms were cleared from the lungs. Adult mice cohoused for only 1 week also contained P. carinii f. sp. muris cysts detectable by silver staining at 5 and 6 weeks after the beginning of cohousing. We also found that the P. carinii f. sp. muris organisms grew to greater numbers in the lungs of BALB/c mice than in those of C57BL6 mice. This indicates that immunocompetent hosts develop a mild infection with P. carinii f. sp. muris which resolves in 5 to 6 weeks when there is a detectable immune response to the organism. Once an acquired immune response was initiated, the P. carinii f. sp. muris organisms were quickly eliminated without clinical signs of disease. PMID:12654827

An, Chun Li; Gigliotti, Francis; Harmsen, Allen G

2003-04-01

379

Coral overgrowth by an encrusting red alga ( Ramicrusta sp.): a threat to Caribbean reefs?  

NASA Astrophysics Data System (ADS)

An encrusting red alga ( Ramicrusta sp., Peyssonneliaceae) present in Lac Bay, Bonaire, overgrows and kills corals and other sessile organisms. Living coral tissue comprises 7.2 % of the benthic composition of the shallow reef, while Ramicrusta sp. covers 18.7 % of the substratum. Of 1374 coral colonies surveyed, 45.8 % were partially overgrown by Ramicrusta sp., with P. porites, P. astreoides and M. complanata being the most susceptible to overgrowth. Mean Ramicrusta sp. maximum overgrowth rates ± SD were 0.08 ± 0.05 mm d-1, 0.07 ± 0.03 mm d-1 and 0.06 ± 0.02 mm d-1 for M. complanata, P. porites and P. astreoides, respectively. None of the 71 coral recruits surveyed were growing on Ramicrusta sp. Ramicrusta sp. is an immediate threat to corals, reduces the area of suitable substratum for coral settlement and may have the ability to influence coral species composition.

Eckrich, Caren E.; Engel, M. Sabine

2013-03-01

380

Identification and molecular cloning of Xenopus laevis SP22, a protein associated with fertilization in mammals  

Microsoft Academic Search

SP22 is a novel sperm protein that has been shown to be highly correlated with fertility in rats. SP22 homologues have been studied in mouse and man but a definitive role for the protein has not yet been established. Using a polyclonal IgG to recombinant rat SP22, we detected the presence of this protein in Xenopus laevis tissues. Moreover, a

Claudio Monetti; Davide Vigetti; Rosalba Gornati; Mariangela Prati; Gary R Klinefelter; Giovanni Bernardini

2002-01-01

381

Direct evaluation of the sp 3 content in diamond-like-carbon films by XPS  

Microsoft Academic Search

The analysis of the X-ray photoelectron spectra (XPS) of the C 1s core level of pulsed laser deposited diamond-like carbon thin films, obtained at different laser intensities is presented. These spectra are deconvoluted into two different contributions, at 284.4 eV and 285.2 eV, which are respectively attributed to sp2 and sp3 hybridized carbon atoms. From the deconvoluted spectra, the sp3

P. Mérel; M Tabbal; M Chaker; S Moisa; J Margot

1998-01-01

382

THE FISCHER-CLIFFORD MATRICES OF THE GROUP 2: SP6(2)  

Microsoft Academic Search

The smallest Fischer sporadic simple group F22 has 14 maximal subgroups up to conjugacy as listed in [2] and [15]. The group 2: SP6(2) is a maximal subgroup of F22 of index 694980. We use the technique of the Fischer-Clifford matrices to constuct the character table of the group 2: SP6(2). The Fischer-Clifford matrices of 2: SP6(2) are used, together

Jamshid Moori; Zwelethemba Mpono

1999-01-01

383

Perturbation of DPPC bilayers by high concentrations of pulmonary surfactant protein SP-B  

Microsoft Academic Search

Deuterium ( 2H) NMR has been used to observe perturbation of dipalmitoylphosphatidylcholine (DPPC) bilayers by the pulmonary surfactant protein B (SP-B) at concentrations up to 17% (w\\/w). Previous 2H NMR studies of DPPC\\/dipalmitoylphosphatidylglycerol (DPPG) (7:3) bilayers containing up to 11% (w\\/w) SP-B and DPPC bilayers containing up to 11% (w\\/w) synthetic SP-B indicated a slight effect on bilayer chain order

Michael R. Morrow; June Stewart; Svetla Taneva; Awel Dico; Kevin M. W. Keough

2004-01-01

384

Transcriptional regulation of the hepatocyte growth factor (HGF) gene by the Sp family of transcription factors.  

PubMed

In this study, we have localized an enhancer element in the 5'-flanking region of the HGF gene promoter and have identified its functional transcriptional factors. Through transient transfection of NIH3T3 fibroblast cells and gel mobility shift assays, the functional binding site was localized to a short region (-318 to -303 bp from the transcription start site) which has a CTCCC sequence. This motif is also conserved in the human HGF promoter and acts as a binding site for the Sp family of transcription factors. In the presence of NIH3T3 nuclear protein extracts, this enhancer region formed specific DNA-protein complexes which were totally abrogated by excess amounts of consensus Sp1 oligonucleotide. In gel mobility supershift assays, anti-Sp1 and anti-Sp3 antibodies specifically recognized these complexes as was evident by their slower migration. Site-specific mutation of this binding motif resulted in total loss of Sp1 and Sp3 binding and a concomitant loss of enhancer function within the context of the HGF promoter. Furthermore, in transient cotransfection assays, overexpression of Sp1 and/or Sp3 stimulated HGF promoter activity independently and additively through binding to the Sp1 binding site in the HGF gene promoter region. DNaseI hypersensitive analysis of freshly isolated nuclei from NIH3T3 cells revealed that five hypersensitive sites (HSS) are present within the 2 kb region immediately upstream of the HGF promoter. One of these sites was mapped to position -0.3 kb from the transcription start site. These data show that both Sp1 and Sp3 transcription factors upregulate HGF promoter activity by binding to the Sp1 binding site at position -318 to -303 bp in the HGF gene promoter. PMID:9223667

Jiang, J G; Chen, Q; Bell, A; Zarnegar, R

1997-06-26

385

A 3-D time-dependent unstructured tetrahedral-mesh SP{sub N} method  

SciTech Connect

We have developed a 3-D time-dependent multigroup SP{sub n} method for unstructured tetrahedral meshes. The SP{sub n} equations are expressed in a canonical form which allows them to be solved using standard diffusion solution techniques in conjunction with source iteration, diffusion-synthetic acceleration, and fission-source acceleration. A computational comparison of our SP{sub n} method with an even-parity S{sub n} method is given.

Morel, J.E.; McGhee, J.M. [Los Alamos National Lab., NM (United States); Larsen, E.W. [Michigan Univ., Ann Arbor, MI (United States). Dept. of Nuclear Engineering

1994-10-01

386

Bcl2 overexpression attenuates SP600125-induced apoptosis in human leukemia U937 cells  

Microsoft Academic Search

SP600125 is a specific inhibitor of c-Jun N-terminal kinase (JNK) that is known to strongly induce apoptosis and block cell cycle progression in G2\\/M phase. In this study, we demonstrated that treatment of U937 cells with SP600125 resulted in significant G2\\/M cell cycle arrest that was due to decreased cyclin B1 and cdc25c protein levels. Moreover, SP600125 promoted LDH release

Dong-Oh Moon; Mun-Ock Kim; Yung Hyun Choi; Nam Deuk Kim; Jeong-Hyun Chang; Gi-Young Kim

2008-01-01

387

Effect of hydrophobic surfactant peptides SP-B and SP-C on binary phospholipid monolayers. I. Fluorescence and dark-field microscopy.  

PubMed Central

The influence of the hydrophobic proteins SP-B and SP-C, isolated from pulmonary surfactant, on the morphology of binary monomolecular lipid films containing phosphocholine and phosphoglycerol (DPPC and DPPG) at the air-water interface has been studied using epifluorescence and dark-field microscopy. In contrast to previously published studies, the monolayer experiments used the entire hydrophobic surfactant protein fraction (containing both the SP-B and SP-C peptides) at physiologically relevant concentrations (approximately 1 wt %). Even at such low levels, the SP-B/C peptides induce the formation of a new phase in the surface monolayer that is of lower intrinsic order than the liquid condensed (LC) phase that forms in the pure lipid mixture. This presumably leads to a higher structural flexibility of the surface monolayer at high lateral pressure. Variation of the subphase pH indicates that electrostatic interaction dominates the association of the SP-B/C peptides with the lipid monolayer. As evidenced from dark-field microscopy, monolayer material is excluded from the DPPC/DPPG surface film on compression and forms three-dimensional, surface-associated structures of micron dimensions. Such exclusion bodies formed only with SP-B/C peptides. This observation provides the first direct optical evidence for the squeeze-out of pulmonary surfactant material in situ at the air-water interface upon increasing monolayer surface pressures.

Kruger, P; Schalke, M; Wang, Z; Notter, R H; Dluhy, R A; Losche, M

1999-01-01

388

Combined and Independent Action of Proteins SP-B and SP-C in the Surface Behavior and Mechanical Stability of Pulmonary Surfactant Films  

PubMed Central

The hydrophobic proteins SP-B and SP-C are essential for pulmonary surfactant function, even though they are a relatively minor component (<2% of surfactant dry mass). Despite countless studies, their specific differential action and their possible concerted role to optimize the surface properties of surfactant films have not been completely elucidated. Under conditions kept as physiologically relevant as possible, we tested the surface activity and mechanical stability of several surfactant films of varying protein composition in vitro using a captive bubble surfactometer and a novel (to our knowledge) stability test. We found that in the naturally derived surfactant lipid mixtures, surfactant protein SP-B promoted film formation and reextension to lower surface tensions than SP-C, and in particular played a vital role in sustaining film stability at the most compressed states, whereas SP-C produced no stabilization. Preparations containing both proteins together revealed a slight combined effect in enhancing film formation. These results provide a qualitative and quantitative framework for the development of future synthetic therapeutic surfactants, and illustrate the crucial need to include SP-B or an efficient SP-B analog for optimal function.

Schurch, David; Ospina, Olga L.; Cruz, Antonio; Perez-Gil, Jesus

2010-01-01

389

Changes in gene expression induced by Sp1 knockdown differ from those caused by challenging Sp1 binding to gene promoters.  

PubMed

C/G-rich DNA regions, which include those recognized by the Sp1 transcription factor in several gene promoters, also encompass potential binding sites for the DNA-intercalating anthracyclines doxorubicin and WP631. We explored the differences between changes in gene expression caused by the ability of these drugs to compete with Sp1 for binding to DNA and those produced by Sp1 knockdown. By quantitative RT-PCR of around 100 genes, most of them involved in control of cell cycle progression, we found that the treatment of human MDA-MB231 breast carcinoma cells with bis-anthracycline WP631 for 24 h produced a profile of gene down-regulation markedly different from the profile caused by doxorubicin treatment or by stable Sp1 knockdown. These observations are rationalized by considering a near-specific effect of WP631 on Sp1 interaction with several gene promoters, thus representing potential therapeutic targets for WP631, in contrast to a less specific effect of reducing the availability of Sp1 through RNA interference. Genes down-regulated upon each treatment were mapped to their molecular and biological functions, which documented the down-regulation, among other things, of genes involved in mRNA transcription regulation, granting us insights into the effects of challenging the transactivation of gene expression by Sp1. PMID:21684359

Mansilla, Sylvia; Priebe, Waldemar; Portugal, José

2011-06-13

390

Sp1 and Sp3 regulate transcription of the cyclin-dependent kinase 5 regulatory subunit 2 (p39) promoter in neuronal cells  

PubMed Central

Cyclin dependent kinase 5 (cdk5) activity is critical for development and function of the nervous system. Cdk5 activity is dependent on association with the regulators p35 and p39 whose expression is highly regulated in the developing nervous system. We have identified a small 200bp fragment of the p39 promoter that is sufficient for cell type-specific expression in neuronal cells. Mutational analysis revealed that a cluster of predicted binding sites for Sp1, AP-1/CREB/ATF and E box-binding transcription factors is essential for full activity of the p39 promoter. Electrophoretic mobility shift assays revealed that Sp1 and Sp3 bound to sequences required for p39 promoter function and chromatin immunoprecipitation assays confirmed binding of these proteins to the endogenous p39 promoter. Furthermore, depletion of either Sp1 or Sp3 by siRNA reduced expression from the p39 promoter. Our data suggest that the ubiquitously expressed transcription factors Sp1 and Sp3 regulate transcription of the cdk5 regulator p39 in neuronal cells, possibly in cooperation with tissue-specific transcription factors.

Valin, Alvaro; Cook, Julie D.; Ross, Sarah; Saklad, Christi L.; Gill, Grace

2009-01-01

391

Aspirin Inhibits Colon Cancer Cell and Tumor Growth and Downregulates Specificity Protein (Sp) Transcription Factors  

PubMed Central

Acetylsalicylic acid (aspirin) is highly effective for treating colon cancer patients postdiagnosis; however, the mechanisms of action of aspirin in colon cancer are not well defined. Aspirin and its major metabolite sodium salicylate induced apoptosis and decreased colon cancer cell growth and the sodium salt of aspirin also inhibited tumor growth in an athymic nude mouse xenograft model. Colon cancer cell growth inhibition was accompanied by downregulation of Sp1, Sp3 and Sp4 proteins and decreased expression of Sp-regulated gene products including bcl-2, survivin, VEGF, VEGFR1, cyclin D1, c-MET and p65 (NF?B). Moreover, we also showed by RNA interference that ?-catenin, an important target of aspirin in some studies, is an Sp-regulated gene. Aspirin induced nuclear caspase-dependent cleavage of Sp1, Sp3 and Sp4 proteins and this response was related to sequestration of zinc ions since addition of zinc sulfate blocked aspirin-mediated apoptosis and repression of Sp proteins. The results demonstrate an important underlying mechanism of action of aspirin as an anticancer agent and, based on the rapid metabolism of aspirin to salicylate in humans and the high salicylate/aspirin ratios in serum, it is likely that the anticancer activity of aspirin is also due to the salicylate metabolite.

Pathi, Satya; Jutooru, Indira; Chadalapaka, Gayathri; Nair, Vijayalekshmi; Lee, Syng-Ook; Safe, Stephen

2012-01-01

392

[Significance of fetal arterial oxygen saturation (SpO2) monitoring].  

PubMed

The significance of fetal arterial oxygen saturation (SpO2) monitoring and its relationship with FHR patterns were studied. Continuous fetal SpO2 was obtained by infrared pulse oximetry (Nellcor N-400) in 76 cases. After amniotomy, a reflectance fetal oximetry sensor (Nellcor FS-10) was placed onto the fetal cheek. The relationship between SpO2 and FHR pattern was then studied and the following results were obtained. 1. Mean recording time was 218 min. and satisfactory records were obtained during 75% of that time. 2. Whenever the FHR was reassuring, the range of SpO2 was stable within 40-80%. 3. Variations in SpO2 values were noted during decelerative patterns. When the FHR was not reassuring, the SpO2 value tended to be lower than 40%. A more acidotic tendency was found in cases where SpO2 was below 40% prior to delivery (umbilical arterial pH 7.135 +/- 0.100 vs 7.282 +/- 0.047, p < 0.005). 4. In cases of fetal arrhythmia (congenital complete heart block 3, supra ventricular tachycardia 1), SpO2 monitoring seemed to be an effective tool for fetal surveillance during labor. Fetal SpO2 measurement seemed to be an alternative to scalp blood sampling and is especially effective in cases with a nonreassuring or uninterpretable FHR pattern such as fetal arrhythmia. PMID:8718544

Amano, K; Hirano, S; Nishijima, M

1996-02-01

393

Vibrational properties of sp carbon atomic wires in cluster-assembled carbon films  

NASA Astrophysics Data System (ADS)

Linear chains made by a single row of sp-hybridized carbon are predicted to display fascinating mechano-electronic properties connected with their termination and stabilization inside realistic carbon structures. The present work describes how the computed vibrational properties of cumulenic and polyynic carbon chains allow one to interpret the carbynic features observed in Raman spectra of cluster-assembled sp-sp2 films. The overall picture is consistent with the measured decay of the sp components induced by air or oxygen exposure.

Onida, Giovanni; Manini, Nicola; Ravagnan, Luca; Cinquanta, Eugenio; Sangalli, Davide; Milani, Paolo

2010-06-01

394

Stramenopile microorganisms associated with the massive coral Favia sp.  

PubMed

The surfaces of massive corals of the genus Favia from Eilat, Red Sea, and from Heron Island, Great Barrier Reef, are covered by a layer of eukaryotic microorganisms. These microorganisms are embedded in the coral mucus and tissue. In the Gulf of Eilat, the prevalence of corals covered by patches of eukaryotic microorganisms was positively correlated with a decrease in water temperatures (from 25-28 degrees C in the summer to 20-23 degrees C in winter). Comparisons carried out using transmission and scanning electron microscopy showed morphological similarities between the microorganisms from the two geographically distant reefs. The microorganisms found on and in the tissues were approximately 5-15 microm in diameter, surrounded by scales in their cell wall, contained a nucleus, and included unique auto-florescent coccoid bodies of approximately 1 mum. Such morphological characters suggested that these microorganisms are stramenopile protists and in particular thraustochytrids. Molecular analysis, carried out using specific primers for stramenopile 18S rRNA genes, revealed that 90% (111/123) of the clones in the gene libraries were from the Thraustochytriidae. The dominant genera in this family were Aplanochytrium sp., Thraustochytrium sp., and Labyrinthuloides sp. Ten stramenopile strains were isolated and cultured from the corals. Some strains showed > or =97% similarity to clones derived from libraries of mucus-associated microorganisms retrieved directly from these corals. Fatty acid characterization of one of the prevalent strains revealed a high percentage of polyunsaturated fatty acids, including omega-3. The possible association of these stramenopiles in the coral holobiont appeared to be a positive one. PMID:20236189

Siboni, Nachshon; Rasoulouniriana, Diana; Ben-Dov, Eitan; Kramarsky-Winter, Esti; Sivan, Alex; Loya, Yossi; Hoegh-Guldberg, Ove; Kushmaro, Ariel

2010-03-03

395

Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.  

PubMed

This study describes actinobacteria isolated from the marine sponge Haliclona sp. collected in shallow water of the South China Sea. A total of 54 actinobacteria were isolated using media selective for actinobacteria. Species diversity and natural product diversity of isolates from marine sponge Haliclona sp. were analysed. Twenty-four isolates were selected on the basis of their morphology on different media and assigned to the phylum Actinobacteria by a combination of 16S rRNA gene based restriction enzymes digestion and 16S rRNA gene sequence analysis. The 16S rRNA genes of 24 isolates were digested by restriction enzymes TaqI and MspI and assigned to different groups according to their restriction enzyme pattern. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belonged to the genera Streptomyces, Nocardiopsis, Micromonospora and Verrucosispora; one other isolate was recovered that does not belong to known genera based on its unique 16S rRNA gene sequence. To our knowledge, this is the first report of a bacterium classified as Verrucosispora sp. that has been isolated from a marine sponge. The majority of the strains tested belong to the genus Streptomyces and three isolates may be new species. All of the 24 isolates were screened for genes encoding polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). PKS and NRPS sequences were detected in more than half of the isolates and the different "PKS-I-PKS-II-NRPS" combinations in different isolates belonging to the same species are indicators of their potential natural product diversity and divergent genetic evolution. PMID:17566868

Jiang, Shumei; Sun, Wei; Chen, Minjie; Dai, Shikun; Zhang, Long; Liu, Yonghong; Lee, Kyung Jin; Li, Xiang

2007-06-14

396

Human glycolipid transfer protein gene (GLTP) expression is regulated by Sp1 and Sp3: involvement of the bioactive sphingolipid ceramide.  

PubMed

Glycolipid transfer protein (GLTP) accelerates glycolipid intermembrane transfer via a unique lipid transfer/binding fold (GLTP fold) that defines the GLTP superfamily and is the prototype for functional GLTP-like domains in larger proteins, i.e. FAPP2. Human GLTP is encoded by the single-copy GLTP gene on chromosome 12 (12q24.11 locus), but regulation of GLTP gene expression remains completely unexplored. Herein, the ability of glycosphingolipids (and their sphingolipid metabolites) to regulate the transcriptional expression of GLTP via its promoter has been evaluated. Using luciferase and GFP reporters in concert with deletion mutants, the constitutive and basal (225 bp; ?78% G+C) human GLTP promoters have been defined along with adjacent regulatory elements. Despite high G+C content, translational regulation was not evident by the mammalian target of rapamycin pathway. Four GC-boxes were shown to be functional Sp1/Sp3 transcription factor binding sites. Mutation of one GC-box was particularly detrimental to GLTP transcriptional activity. Sp1/Sp3 RNA silencing and mithramycin A treatment significantly inhibited GLTP promoter activity. Among tested sphingolipid analogs of glucosylceramide, sulfatide, ganglioside GM1, ceramide 1-phosphate, sphingosine 1-phosphate, dihydroceramide, sphingosine, only ceramide, a nonglycosylated precursor metabolite unable to bind to GLTP protein, induced GLTP promoter activity and raised transcript levels in vivo. Ceramide treatment partially blocked promoter activity decreases induced by Sp1/Sp3 knockdown. Ceramide treatment also altered the in vivo binding affinity of Sp1 and Sp3 for the GLTP promoter and decreased Sp3 acetylation. This study represents the first characterization of any Gltp gene promoter and links human GLTP expression to sphingolipid homeostasis through ceramide. PMID:20974858

Zou, Xianqiong; Gao, Yongguang; Ruvolo, Vivian R; Gardner, Tawnya L; Ruvolo, Peter P; Brown, Rhoderick E

2010-10-25

397

Benzomalvins, new substance P inhibitors from a Penicillium sp.  

PubMed

In the course of screening microbial broths for neurokinin receptor antagonists, a series of new benzodiazepines, benzomalvins A (1), B (2) and C (3), has been isolated from the culture broth of a fungus identified as a Penicillium sp. Benzomalvin A (1) showed inhibitory activity against substance P with Ki values of 12, 42 and 43 microM at the guinea pig, rat and human neurokinin NK1 receptors, respectively. Benzomalvins B (2) and C (3) were only weakly active. The structures of these compounds were determined by spectroscopic methods including MS measurements and NMR analysis. PMID:7518818

Sun, H H; Barrow, C J; Sedlock, D M; Gillum, A M; Cooper, R

1994-05-01

398

[A new sesquiterpenoid from fungus Colletotrichum sp. and its cytotoxicity].  

PubMed

A novel sesquiterpenoid (1) and three known compounds identified as isoaltenuene (2), altenuene (3), and alternariol 4, 10-O-dimethyl ether (4), were isolated in our investigation of the cytotoxic constituents from solid cultures of the endophytic fungus Colletotrichum sp. The structures of these compounds were elucidated through spectroscopic data analysis. All compounds exhibited cytotoxic activity against lung cancer cell line A549, breast cancer cell line MDA-MB-231 and pancreatic cancer cell line PANC-1. Compound 4 could induce the PANC-1 cells inflation or death, but couldn't induce apoptosis at the IC50 of 60.2 microg x mL(-1). PMID:23984524

Yang, Zhi-Jun; Yang, Tian; Luo, Min-Yu; Xia, Xing; Chen, Dai-Jie; Qian, Xiu-Ping

2013-06-01

399

Synthesis of Highly Crystalline sp(2)-Bonded Boron Nitride Aerogels.  

PubMed

sp(2)-Bonded boron nitride aerogels are synthesized from graphene aerogels via carbothermal reduction of boron oxide and simultaneous nitridation. The color and chemical composition of the original gel change dramatically, while structural features down to the nanometer scale are maintained, suggesting a direct conversion of the carbon lattice to boron nitride. Scanning and transmission electron microscopies reveal a foliated architecture of wrinkled sheets, a unique morphology among low-density, porous BN materials. The converted gels display a high degree of chemical purity (>95%) and crystalline order and exhibit unique cross-linking structures. PMID:24011289

Rousseas, Michael; Goldstein, Anna P; Mickelson, William; Worsley, Marcus A; Woo, Leta; Zettl, Alex

2013-09-12

400

Chromomycin SA analogs from a marine-derived Streptomyces sp.  

PubMed Central

Two chromomycin SA analogs, chromomycin SA3 and chromomycin SA2, along with deacetylchromomycin A3 and five previously reported chromomycin analogs were isolated from a marine-derived Streptomyces sp. The structures of the new compounds were determined by spectroscopic methods including 1D and 2D NMR techniques, HRMS and chemical methods. Chromomycin SA3 and chromomycin SA2 are the first naturally occuring chromomycin analogs with truncated side-chains. Biological evaluation of chromomycin analogs for cytotoxicity against two non-small cell lung cancer (NSCLC) cell-lines, A549 and HCC44, demonstrated a decrease in cytotoxicity for the truncated sides chain chromomycin analogs.

Hu, Youcai; Espindola, Ana Paula D. M; Stewart, Nathan A.; Wei, Shuguang; Posner, Bruce A.; MacMillan, John B.

2011-01-01

401

[Biological activity of Penicillium sp. 10-51 exometabolites].  

PubMed

Silica gel column chromatography (silica gel "L" II kind of activity 100/160 mkm) of the chloroform extract from the cultural filtrate of Penicillium sp. 10-51 gave two fractions (chloroform and chloroform-acetone, 5:1) having biological activity. Recrystallization yielded two compounds. On the basis of physico-chemical and spectral data these compounds were identified as curvularin and hydroxycurvularin, which have a large spectrum of biological action as to bacteria, yeast, blue-green algae and phytopathogenic micromycetes. PMID:23088100

Savchuk, Ia I; Za?chenko, A M; Tsyganenko, E S

402

Halogenated Cyclic Peptides Isolated From the Sponge Corticium sp  

PubMed Central

Fractionation of two Fijian specimens of the sponge Corticium sp., led to the isolation of the known active alkaloid steroid plakinamine A and two new halogenated cyclic peptides, corticiamide A (1) and cyclocinamide B (2). Structural elucidation of 1 and 2 was achieved by an extensive combination of high field NMR and HRFT MS/MS experiments, and the absolute stereochemistry of 2 was determined by acid hydrolysis and Marfey’s analysis. Corticiamide A (1) and cyclocinamide B (2) represent the first peptides to be described from the genus Corticium.

Laird, Damian W.; LaBarbera, Daniel V.; Feng, Xidong; Bugni, Tim S.; Harper, Mary Kay; Ireland, Chris M.

2008-01-01

403

Complete genome sequence of Thioalkalivibrio sp. K90mix.  

PubMed

Thioalkalivibrio sp. K90mix is an obligately chemolithoautotrophic, natronophilic sulfur-oxidizing bacterium (SOxB) belonging to the family Ectothiorhodospiraceae within the Gammaproteobacteria. The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method. Here we report the complete genome sequence of strain K90mix and its annotation. The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams. PMID:22675584

Muyzer, Gerard; Sorokin, Dimitry Y; Mavromatis, Konstantinos; Lapidus, Alla; Foster, Brian; Sun, Hui; Ivanova, Natalia; Pati, Amrita; D'haeseleer, Patrik; Woyke, Tanja; Kyrpides, Nikos C

2011-12-23

404

New Cembrane Diterpenoids from a Hainan Soft Coral Sinularia sp  

PubMed Central

Five new cembrane diterpenoids, named sinuflexibilins A–E (1–5), along with nine other known diterpenoids (6–14), have been isolated from the organic extract of a Hainan soft coral Sinularia sp. Their structures were determined on the basis of extensive spectroscopic analyses and by comparison of their spectral data with those of related metabolites. Compound 13, flexibilide, exhibited significant inhibitory activity of NF-?B activation using the cell-based HEK293 NF-?B luciferase reporter gene assay.

Yang, Bin; Zhou, Xuefeng; Huang, Hui; Yang, Xian-Wen; Liu, Juan; Lin, Xiuping; Li, Xiubao; Peng, Yan; Liu, Yonghong

2012-01-01

405

Oligoneuria macabaiba sp. nov. (Insecta: Ephemeroptera) from Brazil.  

PubMed

The former monotypic genus Oligoneuria Pictet was known solely by a female subimago of the type-species Oligoneuria anomala Pictet. A new species of the genus Oligoneuria from the Atlantic rainforest of Macaé, Rio de Janeiro is described. The description was based in males and females imagos of Oligoneuria macabaiba sp. nov. caught with light traps. This species presents similar wing venation, abdominal posterolateral spines, as well as a membranous extension on anterior portion of the head as seen in the genus. Based on features of the new species, the genus is herein redefined. The species represents the first record of the genus from Southeastern Brazil. PMID:22208073

Gonçalves, Inês C; Da-Silva, Elidiomar R; Nessimian, Jorge L

2011-12-01

406

Halioxepine, a new meroditerpene from an Indonesian sponge Haliclona sp.  

PubMed

Chemical investigations on a sponge Haliclona sp. found a meroditerpene 1 having a new carbon skeleton. By analyzing spectroscopic data, the structure was elucidated to comprise a substituted hydroquinone, a tetrahydrooxepine, and a cyclohexene, and these components were united with C1 and C2 units. Compound 1 showed moderate cytotoxicity against NBT-T2 cells with IC50 4.8 µg/ml and also antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 3.2 µg/ml. PMID:21963646

Trianto, Agus; Hermawan, Idam; de Voogd, Nicole Joy; Tanaka, Junichi

2011-01-01

407

Renierosides, cerebrosides from a marine sponge Haliclona (Reniera) sp.  

PubMed

Guided by the brine shrimp lethality assay, eight new cerebrosides (1-8) have been isolated from an extract of the marine sponge Haliclona (Reniera) sp. A novel feature of these cerebrosides was the presence of unprecedented amide-linked long-chain fatty acid moieties. The planar structures of the cerebrosides (1-8) were established by 1D and 2D NMR spectroscopic techniques, mass spectrometric analyses, and chemical degradation methods. The isolated compounds did not display cytotoxicity to a panel of five human solid tumor cell lines. PMID:17848089

Mansoor, Tayyab A; Shinde, Pramod B; Luo, Xuan; Hong, Jongki; Lee, Chong-O; Sim, Chung Ja; Son, Byeng Wha; Jung, Jee H

2007-09-12

408

Polyketides from a Marine-Derived Fungus Xylariaceae sp.  

PubMed Central

Eighteen polyketides (1–18) including six citrinin derivatives, two phenol derivatives, one cyclopentenone, two naphthol derivatives, and seven tetralone derivatives were isolated from the culture broth of a marine-derived fungal strain Xylariaceae sp. SCSGAF0086. Five of these compounds (1, 2, 8, 9, and 10) were new, and their structures were determined by spectroscopic methods. Compounds 4, 6, 7, and 17 showed enzyme-inhibitory activities towards several tested enzymes, and 6 and 7 showed strong antifouling activity against Bugula neritina larvae settlement. This is the first time that the antifouling and enzyme-inhibitory activities of these compounds has been reported.

Nong, Xu-Hua; Zheng, Zhi-Hui; Zhang, Xiao-Yong; Lu, Xin-Hua; Qi, Shu-Hua

2013-01-01

409

Streptothricin derivatives from Streptomyces sp. I08A 1776.  

PubMed

Five new streptothricin derivatives with a carbamoyl group substituted at C-12 (1-5) and three known analogues have been isolated from the culture broth of Streptomyces sp. I08A 1776 by ion exchange and hydrophilic interaction chromatographic techniques. Their structures were determined by spectroscopic and chemical methods. Compound 3 was a streptothricin derivative possessing a cis-streptolidine moiety. Its absolute configuration was defined by comparison of quantum chemical TDDFT calculated and experimental ECD spectra. Compound 5 and streptothricin E (6) displayed antibacterial and antifungal activity with MIC values in the range 1-64 ?g/mL. PMID:21510638

Gan, Maoluo; Zheng, Xudong; Gan, Lishe; Guan, Yan; Hao, Xueqin; Liu, Yishuang; Si, Shuyi; Zhang, Yuqin; Yu, Liyan; Xiao, Chunling

2011-04-21

410

Discovery of New Eunicellins from an Indonesian Octocoral Cladiella sp.  

PubMed Central

Two new 11-hydroxyeunicellin diterpenoids, cladieunicellin F (1) and (–)-solenopodin C (2), were isolated from an Indonesian octocoral Cladiella sp. The structures of eunicellins 1 and 2 were established by spectroscopic methods, and eunicellin 2 was found to be an enantiomer of the known eunicellin solenopodin C (3). Eunicellin 2 displayed inhibitory effects on the generation of superoxide anion and the release of elastase by human neutrophils. The previously reported structures of two eunicellin-based compounds, cladielloides A and B, are corrected in this study.

Chen, Yung-Husan; Tai, Chia-Ying; Su, Yin-Di; Chang, Yu-Chia; Lu, Mei-Chin; Weng, Ching-Feng; Su, Jui-Hsin; Hwang, Tsong-Long; Wu, Yang-Chang; Sung, Ping-Jyun

2011-01-01

411

Chemical components from the Vietnamese soft coral Lobophytum sp.  

PubMed

Chromatographic separation resulted in the identification of one new squalene derivative, named lobophytene (1), three cembranoid diterpenes (2-4), and two sterols (5 and 6) from the Vietnamese marine soft coral Lobophytum sp. Their structures were identified on the basis of extensive spectroscopic data and comparison of those with reported data. Compounds 1 and 2 showed significant cytotoxic activities against lung (A549) and colon (HT-29) cell lines with IC(50) values of 8.2 and 5.6 microM for 1; 5.1 and 1.8 microM for 2, respectively. PMID:20422357

Nguyen, Huu Tung; Chau, Van Minh; Phan, Van Kiem; Hoang, Thanh Huong; Nguyen, Hoai Nam; Nguyen, Xuan Cuong; Tran, Hong Quang; Nguyen, Xuan Nhiem; Hyun, Jae-Hee; Kang, Hee-Kyoung; Kim, Young Ho

2010-04-27

412

Polyketides from a marine-derived fungus Xylariaceae sp.  

PubMed

Eighteen polyketides (1-18) including six citrinin derivatives, two phenol derivatives, one cyclopentenone, two naphthol derivatives, and seven tetralone derivatives were isolated from the culture broth of a marine-derived fungal strain Xylariaceae sp. SCSGAF0086. Five of these compounds (1, 2, 8, 9, and 10) were new, and their structures were determined by spectroscopic methods. Compounds 4, 6, 7, and 17 showed enzyme-inhibitory activities towards several tested enzymes, and 6 and 7 showed strong antifouling activity against Bugula neritina larvae settlement. This is the first time that the antifouling and enzyme-inhibitory activities of these compounds has been reported. PMID:23697953

Nong, Xu-Hua; Zheng, Zhi-Hui; Zhang, Xiao-Yong; Lu, Xin-Hua; Qi, Shu-Hua

2013-05-21

413

Secondary metabolites from fungus Alternaria sp. CIB 108  

Microsoft Academic Search

A new compound, 3-butyryl-6-[rel-(1S, 2S)-1,2-dihydroxypropyl]-4-hydroxy-2H-pyran-2-one (1), was isolated from the solid fermented rice culture of Alternaria sp. CIB 108, along with three known ones, deoxyradicinin (2), 3-epiradicinol (3), and 3-epideoxyradicinol (4). Their structures were elucidated on the basis of spectroscopic data. The single crystal X-ray diffraction was applied to further clarify the structure of 1. Furthermore, compounds 1–4 possessed no

Qian Feng Chen; Min Zhou; Tao Yang; Xiao Zhen Chen; Chun Wang; Guo Lin Zhang; Guo You Li

2011-01-01

414

Complete genome sequence of Thioalkalivibrio sp. K90mix  

PubMed Central

Thioalkalivibrio sp. K90mix is an obligately chemolithoautotrophic, natronophilic sulfur-oxidizing bacterium (SOxB) belonging to the family Ectothiorhodospiraceae within the Gammaproteobacteria. The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method. Here we report the complete genome sequence of strain K90mix and its annotation. The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.

Muyzer, Gerard; Sorokin, Dimitry Y.; Mavromatis, Konstantinos; Lapidus, Alla; Foster, Brian; Sun, Hui; Ivanova, Natalia; Pati, Amrita; D'haeseleer, Patrik; Woyke, Tanja; Kyrpides, Nikos C.

2011-01-01

415

Polysaccharide extraction from Spirulina sp. and its antioxidant capacity.  

PubMed

To optimize polysaccharide extraction from Spirulina sp., the effect of solid-to-liquid ratio, extraction temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that extraction temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, temperature of 90°C, and time of 120 min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45 mg GAE/g sample. PMID:23541559

Chaiklahan, Ratana; Chirasuwan, Nattayaporn; Triratana, Panya; Loha, Veara; Tia, Suvit; Bunnag, Boosya

2013-03-26

416

Antifouling eunicellin-type diterpenoids from the gorgonian Astrogorgia sp.  

PubMed

Twelve new eunicellin-based diterpenoids, astrogorgins B-M (1-12), were isolated from a Chinese gorgonian Astrogorgia sp., together with ophirin, muricellin, astrogorgin, calicophirins A and B, and 14-deacetoxycalicophirin B. The structures of the new compounds were elucidated by extensive spectroscopic analysis and by comparison with data reported in the literature. Significant antifouling activity was observed for 14-deacetoxycalicophirin B against the larval settlement of the barnacle Balanus amphitrite at nontoxic concentrations with an EC(50) = 0.59 ?g/mL, while the other analogues were effective within an EC(50) range of 5.14-17.8 ?g/mL. PMID:22905736

Lai, Daowan; Liu, Dong; Deng, Zhiwei; van Ofwegen, Leen; Proksch, Peter; Lin, Wenhan

2012-08-20

417

Singlet-assisted supersymmetry breaking for Sp(2N) theories  

NASA Astrophysics Data System (ADS)

We investigate local supersymmetry-breaking vacua in s-confining theories with gauge group Sp(2N). By adapting the general recipe developed by Shadmi and Shirman, we construct a realistic model based on dynamics of SQCD coupled with singlets which allows a spontaneously broken supersymmetry. Since the chiral superfields in model have R-charges R = 0 and R = 2 only, the tedious computations of Coleman-Weinberg potential can be greatly alleviated from the lesson of David Shih. We observe that the pseudomoduli fields are stabilized at the origin of moduli space at one-loop order with calculability being preserved.

Zhu, Bin; Meng, Kun; Ding, Ran; Li, Qiyi

2013-07-01

418

Trypanosoma kansasensis sp. n. from Neotoma floridana in Kansas.  

PubMed

Trypanosoma kansasensis sp. n. (Sarcomastigophora: Kinetoplastida) is described from three of 23 (13%) eastern woodrats (Neotoma floridana) collected from Pottawatomie County, Kansas (USA). All flagellates found in the blood of woodrats were trypomastigotes and are larger than T. neotomae in overall dimensions, especially flagellar length and the distance between the posterior end of the organism and kinetoplast. Liver infusion-tryptose (LIT) cultures of infected whole blood resulted in the transformation of some parasites into epimastigotes; however, there was no apparent increase in parasite numbers. PMID:2668570

Upton, S J; Fridell, R A; Tilley, M

1989-07-01

419

Bioconversion of compactin into pravastatin by Streptomyces sp.  

PubMed

Streptomyces sp. Y-110, isolated from soil, modified compactin to pravastatin, a therapeutic agent for hypercholesterolemia. In a batch culture, the highest production of pravastatin was 340 mg l(-1) from 750 mg compactin l(-1) in 24 h. By intermittent feeding of compactin into the culture medium, both the compactin concentration and its conversion increased to 2000 mg l(-1) and 1000 mg pravastatin l(-1), respectively, with the conversion rate of 10 mg l(-1) h(-1). Continuous feeding of compactin increased production of pravastatin to 15 mg l(-1) h(-1). PMID:14677706

Park, Joo-Woong; Lee, Joo-Kyung; Kwon, Tae-Jong; Yi, Dong-Hee; Kim, Young-Jun; Moon, Seong-Hoon; Suh, Hyun-Hyo; Kang, Sang-Mo; Park, Yong-Il

2003-11-01

420

Two new antimicrobial metabolites from the endophytic fungus, Seimatosporium sp.  

PubMed

Two new acaranoic acids, named seimatoporic acid A and B (1, and 2), together with six known compounds, R-(-)-mellein (3), cis-4-hydroxymellein (4), trans-4-hydroxymellein (5), 4R-hydroxy-5-methylmellein (6), (-)-5-hydroxymethylmellein (7), and ergosterol (8) were isolated from an endophytic fungus, Seimatosporium sp, by a bioassay-guided procedure. The structures of the new compounds have been assigned from analysis of the 1H and 13C NMR spectra, DEPT, and by 2D COSY, HMQC, HMBC and NOESY experiments. A mixture of compounds 1 and 2 showed strong antifungal activity against Botrytis cinerea, Septoria tritici, and Pyricularia oryzae. PMID:22545398

Hussain, Hidayat; Krohn, Karsten; Schulz, Barbara; Draeger, Siegfried; Nazir, Mamona; Saleem, Muhammad

2012-03-01

421

Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64  

Microsoft Academic Search

Background, aim, and scope  Indiscriminate use of insecticides leads to environmental problems and poses a great threat to beneficial microorganisms.\\u000a The aim of the present work was to study chlorpyrifos degradation by a rice field cyanobacterium Synechocystis sp. strain PUPCCC 64 so that the organism is able to reduce insecticide pollution in situ.\\u000a \\u000a \\u000a \\u000a \\u000a Material and methods  The unicellular cyanobacterium isolated and purified

D. P. Singh; J. I. S. Khattar; J. Nadda; Y. Singh; A. Garg; N. Kaur; A. Gulati

422

Genome sequence and description of Aeromicrobium massiliense sp. nov.  

PubMed Central

Aeromicrobium massiliense strain JC14Tsp. nov. is the type strain of Aeromicrobium massiliense sp. nov., a new species within the genus Aeromicrobium. This strain, whose genome is described here, was isolated from the fecal microbiota of an asymptomatic patient. Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

Ramasamy, Dhamodharan; Kokcha, Sahare; Lagier, Jean-Christophe; Nguyen, Thi-Thien; Raoult, Didier

2012-01-01

423

Seladonia (Pachyceble) henanensis sp. n. (Hymenoptera, Halictidae) from China.  

PubMed

Seladonia (Pachyceble) henanensis sp. n., is described from Henan Province, the eastern-central part of China. This species is separated from its allied species by a combination of the following morphological characters: head broad in female, inner hind tibial spur of female with 7-8 slender teeth, T1 basolaterally with appressed hair tuft in both sexes, and genitalia with long and large lower gonostylus in male. Important taxonomic characters are illustrated with photographs, scanning electron micrographs, and line drawings. PMID:23794911

Murao, Ryuki; Tadauchi, Osamu; Huan-Li, Xu

2013-05-28

424

Multiplexer/amplifier test results for SP-100  

SciTech Connect

Multiplexer and amplifier systems must be designed with transistors that can perform satisfactorily over ten years to a total gamma dose of 120E6 rads and a total neutron fluence of 1.6E15 nvt for the SP-100 reactor system. Series of gamma and neutron tests have been completed to measure transistor degradation as a function of total dose, fluence, and temperature. Test results indicate that modest increases in temperature result in substantial improvement of transistor performance at a neutron flux of 8E8 n/cm{sup 2}/s. 2 refs., 3 figs.

King, D.B.; Luker, S.M. (Sandia National Labs., Albuquerque, NM (USA)); Ryan, R. (General Electric Co., San Jose, CA (USA))

1990-01-01

425

Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov., leaf-associated bacteria isolated from Jatropha curcas L.  

PubMed

Four orange-pigmented isolates, L7-456, L7-484(T), L9-479 and L9-753(T), originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all four isolates belong to the genus Aureimonas. In these analyses, strain L7-484(T) appeared to be most closely related to Aureimonas ureilytica 5715S-12(T) (95.7?% sequence identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753(T) were found to be identical and also shared the highest similarity with A. ureilytica 5715S-12(T) (97.5?%). Both L7-484(T) and L9-753(T) contained Q-10 and Q-9 as predominant ubiquinones and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sulfoquinovosyldiacylglycerol and an aminophospholipid as the major polar lipids. C18?:?1?7c and C16?:?0 were the major fatty acids. Similar to other species in the genus Aureimonas, hydroxylated fatty acids (e.g. C18?:?1 2-OH) and cyclic fatty acids (C19?:?0 cyclo ?8c) were also present. The DNA G+C contents of L7-484(T) and L9-753(T) were 66.1 and 69.4 mol%, respectively. Strains L7-484(T) and L9-753(T) exhibited less than 40?% DNA-DNA hybridization both between themselves and to A. ureilytica KACC 11607(T). Our results support the proposal that strain L7-484(T) represents a novel species within the genus Aureimonas, for which the name Aureimonas jatrophae sp. nov. is proposed, and that strains L9-753(T), L7-456 (?=?KACC 16229 ?=?DSM 25023) and L9-479 (?=?KACC 16228 ?=?DSM 25024) represent a second novel species within the genus, for which the name Aureimonas phyllosphaerae sp. nov. is proposed. The type strains of Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov. are respectively L7-484(T) (?=?KACC 16230(T) ?=?DSM 25025(T)) and L9-753(T) (?=?KACC 16231(T) ?=?DSM 25026(T)). PMID:22922534

Madhaiyan, M; Hu, C J; Jegan Roy, J; Kim, S-J; Weon, H-Y; Kwon, S-W; Ji, L

2012-08-24

426

Occurrence of a Thiothrix sp. Attached to Mayfly Larvae and Presence of Parasitic Bacteria in the Thiothrix sp  

PubMed Central

Larvae of the mayfly (Drunella grandis [Eaton]) from Diamond Fork Creek, Utah, were covered with a heavy growth of the sulfide-oxidizing bacterium Thiothrix. The bacterium did not seem to harm the mayfly, but the Thiothrix trichomes were parasitized by three morphologically distinct bacteria, two of which were cytoplasmic and one of which was probably periplasmic. At least two of the parasites destroyed the cytoplasmic contents of the Thiothrix sp., thus killing the host cell. Attempts to obtain the parasites in pure culture were unsuccessful. Images

Larkin, John M.; Henk, Margaret C.; Burton, Sheril D.

1990-01-01

427

Spiroplasma atrichopogonis sp. nov., from a ceratopogonid biting midge.  

PubMed

Spiroplasma sp. strain GNAT3597T was isolated from the biting midge genus Atrichopogon (Diptera: Ceratopogonidae). It was serologically distinct from other Spiroplasma species, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a cell membrane (i.e. lacking a cell wall). Growth of strain GNAT3597T occurred in M1D medium at 30 degrees C. Strain GNAT3597T catabolized both glucose and arginine, but did not hydrolyse urea. The DNA G+C content of strain GNAT3597T was 29+/-1 mol%. Only one strain, SMCAT (Spiroplasma mirum), is serologically related to strain GNAT3597T, although the relationship is weak (positive reaction to only a 1 : 80 dilution). It is therefore proposed that strain GNAT3597T (=ATCC BAA-520T=NBRC 100390T) represents a novel species, Spiroplasma atrichopogonis sp. nov. (class Mollicutes: order Entomoplasmatales: family Spiroplasmataceae). PMID:15653889

Koerber, R Thomas; Gasparich, Gail E; Frana, Mark F; Grogan, William L

2005-01-01

428

sp(3)-Bonded silicon allotropes based on the Kelvin problem.  

PubMed

The Kelvin problem, how to partition three-dimensional space into cells of equal volume with minimal area, is a fascinating one. Aggregations of bubbles are naturally physical illustrations of the Kelvin problem. And the superconductor Na8Si46 as an inspiration leads to an amazing discovery of the Weaire-Phelan (WP) structure of foam - the optimal solution to the Kelvin problem to date. Here based on the structural similarity between sp(3)-bonded silicon allotropes and the solutions to the Kelvin problem, a series of new sp(3)-hybridization silicon allotropes, named "Kelvin Silicons", are presented. Furthermore, the structural stability and electronic properties of these new silicon allotropes are investigated using density-functional theory (DFT) calculations. The results show that Kelvin Silicons are structurally stable semiconductors with indirect bandgaps in the range of 0.17-1.40 eV, and their bulk moduli are about 75.9-88.5% that of the diamond phase. The simulated X-ray diffraction spectra of the new silicon crystalline structures would provide more information for possible experimental observations and synthesis. PMID:24036687

Zhao, Hui-Yan; Wang, Jing; Ma, Qing-Min; Liu, Ying

2013-09-25

429

Safety assessment of DHA-rich microalgae from Schizochytrium sp.  

PubMed

The purpose of this series of studies was to assess the genotoxic potential of docosahexaenoic acid-rich microalgae from Schizochytrium sp. (DRM). DRM contains oil rich in highly unsaturated fatty acids (PUFAs). Docosahexaenoic acid (DHA n-3) is the most abundant PUFA component of the oil ( approximately 29% w/w of total fatty acid content). DHA-rich extracted oil from Schizochytrium sp. is intended for use as a nutritional ingredient in foods. All in vitro assays were conducted with and without mammalian metabolic activation. DRM was not mutagenic in the Ames reverse mutation assay using five different Salmonella histidine auxotroph tester strains. Mouse lymphoma suspension assay methodology was found to be inappropriate for this test material because precipitating test material could not be removed by washing after the intended exposure period and the precipitate interfered with cell counting. The AS52/XPRT assay methodology was not subject to these problems and DRM was tested and found not to be mutagenic in the CHO AS52/XPRT gene mutation assay. DRM was not clastogenic to human peripheral blood lymphocytes in culture. Additionally, DRM did not induce micronucleus formation in mouse bone marrow in vivo further supporting its lack of any chromosomal effects. Overall, the results of this series of mutagenicity assays support the conclusion that DRM does not have any genotoxic potential. PMID:12052009

Hammond, Bruce G; Mayhew, Dale A; Kier, Larry D; Mast, Richard W; Sander, Wayne J

2002-04-01

430

Phlebotomus (Euphlebotomus) barguesae n. sp. from Thailand (Diptera - Psychodidae)  

PubMed Central

Background A few studies have been carried out on the Phlebotomine sandflies from Thailand. Within the Phlebotomine sandflies, the genus Phlebotomus Rondani & Berté, 1840 contains the vectors of leishmaniases in Europe, Africa and Asia. It includes several subgenera. Among them the subgenus Euphlebotomus Theodor, 1948 contains at the present time 12 taxa. The type-species of this subgenus is P. argentipes Annandale & Brunetti, 1908, the vector of Leishmania donovani (Laveran & Mesnil, 1903) in India. Results A new species of sandfly, P. barguesae n. sp. is described from limestone caves in Thailand. The male-female gathering in the same species is based on ecological, morphological and molecular criteria (homology of mtDNA cytochrome c oxidase I sequences). The inclusion of P. barguesae n. sp. in the subgenus Euphlebotomus is justified on the basis of characters of the male genitalia (five spines on the style, bifurcated paramere, and no basal lobe on the coxite) and of female pharyngeal armature (two kinds of teeth). It well differenciated from another sympatric species: P. mascomai. Conclusion The new species described in the present study has smooth spermathecae. This original morphology opens a discussion on the heterogeneity of this subgenus.

Depaquit, Jerome; Muller, Frederique; Leger, Nicole

2009-01-01

431

Initial reactions of xanthone biodegradation by an Arthrobacter sp.  

PubMed Central

This study examined the catabolism of xanthone by an Arthrobacter sp. (strain GFB100) capable of growth on xanthone as its main source of carbon and energy. An early catabolic intermediate was 3,4-dihydroxyxanthone. This compound was isolated from the growth medium of a mutant strain of the Arthrobacter sp. which lacked the xanthone-inducible dihydroxyxanthone ring-fission dioxygenase of the wild-type strain. Cell extracts from wild-type xanthone-grown cells oxidized 3,4-dihydroxyxanthone to a yellow ring-fission metabolite. The same yellow compound accumulated in xanthone-grown cultures of a spontaneous mutant which lacked an active, xanthone-inducible, NADPH-linked ring-fission metabolite reductase. The yellow ring-fission metabolite appears to be 4-hydroxy-3-(2'-oxo-3-trans-butenoate)-coumarin, based on its nuclear magnetic resonance spectrum and mass spectral fragmentation pattern, indicating that ring cleavage of 3,4-dihydroxyxanthone was by an extra-diol (meta-fission) mechanism. Enzymatic analyses indicated that growth on xanthone induced a complete gentisate pathway: dioxygenase-catalyzed cleavage of gentisate to maleylpyruvate, isomerization of maleylpyruvate to fumarylpyruvate, and hydrolysis of fumarylpyruvate to fumarate and pyruvate. 4-Hydroxycoumarin was thought to be a likely pathway intermediate linking the early xanthone catabolic steps to the gentisate pathway, since 2-hydroxyacetophenone, a byproduct of 4-hydroxycoumarin hydrolysis, was formed when wild-type cells were cultured with xanthone. Chlorinated 2-hydroxyacetophenones were also obtained from specific chloro-substituted xanthones.

Tomasek, P H; Crawford, R L

1986-01-01

432

Transesterification of triglycerides by dried biomass of Aspergillus sp.  

PubMed

Fungus isolate, Aspergillus sp. (RBD01), which was isolated from biocontaminated clarified butter was evaluated for its potential to transesterify used edible and non-edible oils for generation of alkyl esters, when used as biocatalyst as dry biomass. The work aimed at determining the potential of dry biomass of Aspergillus sp. (RBD01) to transesterify used cottonseed oil and non-edible oils viz., jatropha and karanj under various culture conditions. A conversion of oil (cotton seed) to ethyl ester to the extent of 84% was obtained at reaction temperature of 35°C, with 20% biomass and step-wise addition of ethanol at 1:5 molar ratio (oil to ethanol), within total reaction time of 36 h. Under similar conditions, transesterification of Jatropha and Karanj oils resulted in only 75 and 78.2% ethyl ester. Further, with reference to the effect of frying on transesterification, increase in frying time decreased the extent of transesterification from 84% to 30%. PMID:23648404

Aulakh, Satnam Singh; Prakash, N Tejo; Prakash, Ranjana

2013-01-01

433

Micrococcus lactis sp. nov., isolated from dairy industry waste.  

PubMed

A Gram-positive, yellow-pigmented, actinobacterial strain, DW152(T), was isolated from a dairy industry effluent treatment plant. 16S rRNA gene sequence analysis indicated that strain DW152(T) exhibited low similarity with many species with validly published names belonging to the genera Micrococcus and Arthrobacter. However, phenotypic properties including chemotaxonomic markers affiliated strain DW152(T) to the genus Micrococcus. Strain DW152(T) had ai-C(15:0) and i-C(15:0) as major cellular fatty acids, and MK-8(H(2)) as the major menaquinone. The cell-wall peptidoglycan of strain DW152(T) had l-lysine as the diagnostic amino acid and the type was A4?. The DNA G+C content of strain DW152(T) was 68.0 mol%. In 16S rRNA gene sequence analysis, strain DW152(T) exhibited significant similarity with Micrococcus terreus NBRC 104258(T), but the mean value of DNA-DNA relatedness between these strains was only 42.3%. Moreover, strain DW152(T) differed in biochemical and chemotaxonomic characteristics from M. terreus and other species of the genus Micrococcus. Based on the above differences, we conclude that strain DW152(T) should be treated as a novel species of the genus Micrococcus, for which the name Micrococcus lactis sp. nov. is proposed. The type strain of Micrococcus lactis sp. nov. is DW152(T) (=MTCC10523(T) =DSM 23694(T)). PMID:21239567

Chittpurna; Singh, Pradip K; Verma, Dipti; Pinnaka, Anil Kumar; Mayilraj, Shanmugam; Korpole, Suresh

2011-01-14

434

Methanosaeta sp., the major archaeal endosymbiont of Metopus es.  

PubMed

Epifluorescence microscopy and whole cell in situ hybridization analysis revealed the presence of Methanosaeta sp. as endosymbionts in Metopus es. Direct microscopic observation under epifluorescent microscope showed the presence of long slender rods with an average length of 3.4 microm. The number of methanogenic rods varied from 792 +/- 12 in a single M. es cell with a biovolume of 3.4 x 10(5) microm(3). At the exponential growth stage, a single symbiotic methanogen in M. es produced about 1 fmol methane/h leading to a methane production rate of 0.85 pmol/ciliate/h. The presence of endosymbiotic methanogens in the domain archaea and Methanosaeta sp. was confirmed by FISH with ARCH 915 and MX 825 oligonucleotide probes specific to domain archaea and Methanosaeta respectively. The homogenized cells of M. es also showed bright fluorescing rods with MX 825 hybridization. The culture obtained on inoculation of the released endosymbiotic organisms on Methanosaeta-specific medium lent support to the growth of long slender rods having the same range of mean length (3.6 microm) as that of the endosymbiotic methanogens observed. Both intra- and extracellular production of acetate was detected in M. es culture. PMID:19695326

Narayanan, Nimi; Krishnakumar, Bhaskaran; Anupama, Vijayakumari Nadaraja; Manilal, Vattakkatt Balakrishnan

2009-08-18

435

Pullulanases of alkaline and broad pH range from a newly isolated alkalophilic Bacillus sp. S-1 and a Micrococcus sp. Y-1  

Microsoft Academic Search

Summary Two highly alkalophilic bacteria, and potent producers of alkaline pullulanase, were isolated from Korean soils. The two isolates, identified asBacillus sp. S-1 andMicrococcus sp. Y-1, grow on starch under alkaline conditions and effectively secrete extracellular pullulanases. The two isolates were extremely alkalophilic since bacterial growth and enzyme production occurred at pH values ranging from pH 6.0 to 12.0 forMicrococcus

Cheorl-Ho Kim; Ho-Il Choi; Dae-Sil Lee

1993-01-01

436

Biosorption of lead(II) from aqueous solutions by non-living algal biomass Oedogonium sp. and Nostoc sp.--a comparative study.  

PubMed

Industrial wastewaters containing heavy metals pose a major environmental problem that needs to be remedied. The present study reports the ability of two non-living (dried) fresh water algae, Oedogonium sp. and Nostoc sp. to remove lead(II) from aqueous solutions in batch system under varying range of pH (2.99-7.04), contact time (5-300 min), biosorbent dose (0.1-0.8 g/L), and initial metal ion concentrations (100 and 200mg/L). The optimum conditions for lead biosorption are almost same for the two algal biomass Oedogonium sp. and Nostoc sp. (pH 5.0, contact time 90 and 70 min, biosorbent dose 0.5 g/L and initial Pb(II) concentration 200mg/L) however, the biomass of Oedogonium sp. was found to be more suitable than Nostoc sp. for the development of an efficient biosorbent for the removal of lead(II) from aqueous solutions, as it showed higher values of q(e) adsorption capacity (145.0mg/g for Oedogonium sp. and 93.5mg/g for Nostoc sp.). The equilibrium data fitted well in the Langmuir isotherms than the Freundlich isotherm, thus proving monolayer adsorption of lead on both the algal biomass. Analysis of data shows that the process involves second-order kinetics and thermodynamic treatment of equilibrium data shows endothermic nature of the adsorption process. The spectrum of FTIR confirms that the amino and carboxyl groups on the surface of algal biomass were the main adsorption sites for lead removal. Both the biosorbents could be regenerated using 0.1 mol/L HCl solution, with upto 90% recovery. The biosorbents were reused in five biosorption-desorption cycles without a significant loss in biosorption capacity. Thus, this study demonstrated that both the algal biomass could be used as an efficient biosorbents for the treatment of lead(II) bearing wastewater streams. PMID:18321684

Gupta, Vinod K; Rastogi, Arshi

2008-02-02

437

Lactobacillus uli sp. nov. and Lactobacillus rimae sp. nov. from the Human Gingival Crevice and Emended Descriptions of Lactobacillus minutus and Streptococcus parvulus  

Microsoft Academic Search

Lactobacillus uli sp. nov. and Lactobacillus rimae sp. nov. are described. These organisms are short, gram-positive, strictly anaerobic, rod-shaped bacteria that have DNA G+C contents of 53 and 45 mol%, respectively, produce major amounts of lactic acid, and have been isolated from human gingival crevices and periodontal pockets. The major cellular fatty acid derivatives for both species are Clsz1 cis-9

INGAR OLSEN; JOHN L. JOHNSON; L. V. H. MOORE; W. E. C. MOORE

438

[Thelohanellus njinei sp nov and T lagdoensis sp nov, Myxosporidia (Myxozoa: Myxosporea) parasites of Schilbeidae and Citharinidae fishes in Cameroon (central Africa)].  

PubMed

The study of Myxosporidia infecting Schilbe mystus (Linné, 1758) (Schilbeidae) and Citharinus citharus (Geoffroy Saint-Hilaire, 1809) (Citharinidae) from the Sanaga and Bénoué basins revealed the presence of two new species of the genus Thelohanellus kudo, 1993. These species are Thelohanellus njinei sp. nov. and Thelohanellus lagdoensis sp. nov., found in the gut of Schilbe mystus and Citharinus citharus respectively. New data are also given on the diagnosis of Thelohanellus citharini, a species previously described in Chad. PMID:17645182

Fomena, A; Farikou-Oumarou; Tang, C; Bouix, G

2007-06-01

439

Comparison between natural infection by Cryptosporidium sp., Giardia sp. in dogs in two living situations in the West Zone of the municipality of Rio de Janeiro  

Microsoft Academic Search

In order to compare natural infection by Cyptosporidium sp. and Giardia sp. in clinically healthy dogs living in two situations (animal shelter and household pets), we conducted 166 faecal exams using the technique of centrifugation–flotation in a sugar-saturated solution. Of the faecal samples, 94 came from shelter dogs and 72 from house pets. Eighty-two (49.4%) contained parasite eggs, cysts or

F. Huber; T. C. B. Bomfim; R. S. Gomes

2005-01-01

440

Synthesis of Poly(3-Hydroxybutyrate) from Different Carbon Sources by Haloarcula sp. IRU1  

Microsoft Academic Search

In this study, the ability of the halophilic archaeon Haloarcula sp. IRU1 to utilize 6 different carbon sources (glucose, fructose, sucrose, starch, palmitic acid and sodium acetate) was investigated. A marked increase was observed in the cell concentration and PHB concentration of Haloarcula sp. IRU1 grown in medium containing glucose when compared to other carbon sources, whereas the minimum cell

Mojtaba Taran

2011-01-01

441

Identification and bioinformatic analysis of the membrane proteins of synechocystis sp. PCC 6803  

Microsoft Academic Search

BACKGROUND: The membranes of Synechocystis sp. PCC 6803 play a central role in photosynthesis, respiration and other important metabolic pathways. Comprehensive identification of the membrane proteins is of importance for a better understanding of the diverse functions of its unique membrane structures. Up to date, approximately 900 known or predicted membrane proteins, consisting 24.5% of Synechocystis sp. PCC 6803 proteome,

Yingchun Wang; Wu Xu; Parag R Chitnis

2009-01-01

442

A new species of Thoosa (Demospongiae, Hadromerida) excavating precious coral Corallium sp. from Midway  

Microsoft Academic Search

A new species of Thoosa, T. midwayi n. sp., excavating the scleraxis of Corallium sp. and collected in the Midway Islands, is described on the basis of its spicular complement characterised by two types of amphiasters. The new species differs from the closest species, T. amphiasterina Topsent, in the shape and size of the amphiasters. Although sti