Sample records for species composition total

  1. Composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species.

    PubMed

    Mikulic-Petkovsek, Maja; Schmitzer, Valentina; Slatnar, Ana; Stampar, Franci; Veberic, Robert

    2012-10-01

    Sugars, organic acids, and total phenolic content in fruit of 25 wild and cultivated berry species were identified and quantified with high-performance liquid chromatograph. The composition of sugars, organic acids, and total phenolic compounds in various species of Vaccinium, Rubus, Ribes, and Fragaria genus was evaluated. Additonally, total phenolics of less known berry species of the Morus, Amelanchier, Sorbus, Sambucus, Rosa, Lycium, Actinidia, and Aronia genus were determined in wild growing as well as in cultivated fruits. Significant differences in the concentration of sugars and organic acids were detected among the berry species. Glucose and fructose were the most abundant sugars in berry fruits and the major organic acids were malic and citric acid. However, in kiwi fruit, sucrose represented as much as 71.9% of total sugars. Sorbitol has been detected and quantified in chokeberry, rowanberry, and eastern shadbush fruit. The highest content of total analyzed sugars was determined in rowanberry fruit, followed by dog rose, eastern shadbush, hardy kiwifruit, American cranberry, chokeberry, and jostaberry fruit. Rowanberry stands out as the fruit with the highest content of total analyzed organic acids, followed by jostaberry, lingonberry, red gooseberry, hardy kiwifruit, and black currant. The berries of white gooseberry, black currant, red currant, and white currant had the lowest sugar/organic acid ratio and were thus perceptively the sourest species analyzed. On the other hand, the species with highest sugar/organic acid ratio were goji berry, eastern shadbush, black mulberry, and wild grown blackberry. The highest amounts of total phenols were quantified in chokeberry fruit. Wild strawberry, raspberry, and blackberry had 2- to 5-fold more total phenolics compared to cultivated plants. The fruit of analyzed berry species contained different levels of sugars, organic acids, and total phenolics. Moreover, it has been demonstrated that wild grown species

  2. Species Composition (SC)

    Treesearch

    John F. Caratti

    2006-01-01

    The FIREMON Species Composition (SC) method is used to provide ocular estimates of cover and height measurements for plant species on a macroplot. The SC method provides plant species composition and coverage estimates to describe a stand or plant community and can be used to document changes over time. It is suited for a wide variety of vegetation types and is...

  3. Species richness effects on ecosystem multifunctionality depend on evenness, composition and spatial pattern

    USGS Publications Warehouse

    Maestre, F.T.; Castillo-Monroy, A. P.; Bowker, M.A.; Ochoa-Hueso, R.

    2012-01-01

    1. Recent studies have suggested that the simultaneous maintenance of multiple ecosystem functions (multifunctionality) is positively supported by species richness. However, little is known regarding the relative importance of other community attributes (e.g. spatial pattern, species evenness) as drivers of multifunctionality. 2. We conducted two microcosm experiments using model biological soil crust communities dominated by lichens to: (i) evaluate the joint effects and relative importance of changes in species composition, spatial pattern (clumped and random distribution of lichens), evenness (maximal and low evenness) and richness (from two to eight species) on soil functions related to nutrient cycling (β-glucosidase, urease and acid phosphatase enzymes, in situ N availability, total N, organic C, and N fixation), and (ii) assess how these community attributes affect multifunctionality. 3. Species richness, composition and spatial pattern affected multiple ecosystem functions (e.g. organic C, total N, N availability, β-glucosidase activity), albeit the magnitude and direction of their effects varied with the particular function, experiment and soil depth considered. Changes in species composition had effects on organic C, total N and the activity of β-glucosidase. Significant species richness × evenness and spatial pattern × evenness interactions were found when analysing functions such as organic C, total N and the activity of phosphatase. 4. The probability of sustaining multiple ecosystem functions increased with species richness, but this effect was largely modulated by attributes such as species evenness, composition and spatial pattern. Overall, we found that model communities with high species richness, random spatial pattern and low evenness increased multifunctionality. 5. Synthesis. Our results illustrate how different community attributes have a diverse impact on ecosystem functions related to nutrient cycling, and provide new

  4. Phytoplankton species composition of four ecological provinces in Yellow Sea, China

    NASA Astrophysics Data System (ADS)

    Li, Xiaoqian; Feng, Yuanyuan; Leng, Xiaoyun; Liu, Haijiao; Sun, Jun

    2017-12-01

    The ecological province based on phytoplankton species composition is important to understanding the interplay between environmental parameters and phytoplankton species composition. The aim of this study was to establish phytoplankton species composition ecological pattern thus elucidate the relationship between environmental factors and the phytoplankton species composition in the ecological provinces. Phytoplankton samples were collected from 31 stations in Yellow Sea (121.00°-125.00°E, 32.00°-39.22°N) in November 2014. The samples were enumerated and identified with the Utermöhl method under an optical inverted microscope-AE2000 with magnifications of 200 × or 400 ×. In the present study, a total of 141 taxa belonging to 60 genera of 4 phyla of phytoplankton were identified, among them 101 species of 45 genera were Bacillariophyta, 36 species of 11 genera were Dinophyta, 3 species of 3 genera were Chrysophyta and 1 species of 1 genera was Chlorophyta. The study area was divided into 4 ecological provinces according to an unsupervised cluster algorithm applied to the phytoplankton biomass. A T-S (Temperature-Salinity) scatter diagram depicted with data of water temperature and salinity defined by environmental provinces matched well with the ecological provinces. The results of Canonical Correspondence Analysis (CCA) indicated that the phytoplankton species composition was mainly correlated with temperature, salinity and silicate concentration in the studied area. A method of establishing ecological provinces is useful to further understanding the environmental effects on the marine phytoplankton species composition and the consequent marine biogeochemistry.

  5. [Distribution and changes in species composition and abundance of ichthyoplankton in the Yangtze estuary].

    PubMed

    Zhang, Heng; Yang, Sheng-Long; Meng, Hai-Xing

    2012-06-01

    Based on four surveys of eggs and larvae in the Yangtze estuary in 2005 (April and November) and 2006 (April and September), combined with the historical data of the wetland in 1990 (September) and 1991 (March), we analyzed seasonal changes in fish species composition and quantity of ichthyoplankton. Thirty-six species of egg and larvae were collected and marine fish species were the highest represented ecological guild. Average fish species and average abundance in spring were lower than in autumn for every survey. The total number of eggs in brackish water was higher than in fresh water, but the total number of larvae and juveniles in brackish water was lower. The abundance of eggs and larvae during from 2005 to 2006 in both spring and autumn was higher compared to those from 1990 to 1991. Obvious differences in species composition in September between 1990 and 2006 were found, especially for Erythroculter ilishaeformis and Neosalanx taihuensis. Fish species composition and quantity within the ichthyoplankton community has obviously changed in the Yangtze estuary over the last 20 years.

  6. COMMUNITY SCALE STREAM TAXA SENSITIVITIES TO DIFFERENT COMPOSITIONS OF EXCESS TOTAL DISSOLVED SOLIDS

    EPA Science Inventory

    Model stream chronic dosing studies (42 d) were conducted with three total dissolved solids (TDS) recipes. The recipes differed in composition of major ions. Community scale emergence was compared with single-species responses conducted simultaneously using the whole effluent tox...

  7. [Species composition and spatial structure of plants in urban parks of Beijing].

    PubMed

    Zhao, Juan-Juan; Ouyang, Zhi-Yun; Zheng, Hua; Xu, Wei-Hua; Wang, Xiao-Ke

    2009-02-01

    By the method of stratified random sampling, the species composition and spatial structure of the plants in 53 parks in Beijing urban area were investigated, aimed to provide basic information for the protection of plant diversity in the parks and the management of the parks. A total of 492 plant species belong to 96 families and 283 genera were recorded. Based on the data of 21 investigation items about the trees, shrubs, and grasses in the study area and related statistical analyses, the plant structural patterns commonly seen in the green space of the parks of Beijing urban area were introduced. Among the plants in the parks, native species occupied 53.86% of the total. The chorological composition of the genera embraced broad kinds of geographical elements in China, and the predominance of dominant plants was remarkable. In most green patches of the parks, herbaceous species were more abundant and had higher coverage, shrubs had relatively low coverage and were less beneath tree canopy, and trees and shrubs had lower species richness and density. The canopy breadth and the diameter of breast height of trees as well as the breadth of shrubs and the heights of trees and shrubs were basically at the second grade, but the canopy structure of the trees were better, with good conditions of sunlight and growth. The crown missing of the shrubs was relatively low. It was suggested from correlation analyses and document survey of Beijing parks construction history that park landscape design, alien species introduction; and cultivation management were the main factors affecting the species composition and spatial structure of the plants in Beijing urban parks.

  8. Landbird species composition and relative abundance during migration along the Rio Grande

    Treesearch

    Wang Yong; Deborah M. Finch

    1996-01-01

    In this paper, we report species composition and relative abundances of stopover migrants during spring and fall migration along the middle Rio Grande in 1994. We recorded 157 landbird species using mist-netting and survey methods at two sites on the Rio Grande, the Bosque del Apache and the Rio Grande Nature Center. A total of 6,509 birds was captured during spring...

  9. A Preliminary Survey of Species Composition of Termites (Insecta: Isoptera) in Samunsam Wildlife Sanctuary, Sarawak

    PubMed Central

    Jamil, Norsyarizan; Ismail, Wan Nurainie Wan; Abidin, Siti Shamimi; Amaran, Mazdan Ali; Hazali, Ratnawati

    2017-01-01

    A survey on termite species composition was conducted in Samunsam Wildlife Sanctuary, Sarawak in February 2015. Overall 19 species of termite belonging to 13 genera and 8 subfamilies was found in the sanctuary. It was recorded the subfamily of Termitinae had the highest number of species (6 species, equal to 31.58% of total species), followed by Nasutermitinae (3 species, 15.79%), Macrotermitinae, Amitermitinae, Rhinotermitinae, Coptotermitinae, (2 species, 10.53% respectively), and Heterotermitinae, Termitogetoninae (1 species, 5.26% respectively). Since this rapid survey is the first termite assemblage representation in Samunsam Wildlife Sanctuary, the preliminary result may serve as the baseline data for termite composition in the area. Therefore, a whole coverage for the area within this sanctuary would definitely increase the number of termite species found in the sanctuary. PMID:28890771

  10. A Preliminary Survey of Species Composition of Termites (Insecta: Isoptera) in Samunsam Wildlife Sanctuary, Sarawak.

    PubMed

    Jamil, Norsyarizan; Ismail, Wan Nurainie Wan; Abidin, Siti Shamimi; Amaran, Mazdan Ali; Hazali, Ratnawati

    2017-07-01

    A survey on termite species composition was conducted in Samunsam Wildlife Sanctuary, Sarawak in February 2015. Overall 19 species of termite belonging to 13 genera and 8 subfamilies was found in the sanctuary. It was recorded the subfamily of Termitinae had the highest number of species (6 species, equal to 31.58% of total species), followed by Nasutermitinae (3 species, 15.79%), Macrotermitinae, Amitermitinae, Rhinotermitinae, Coptotermitinae, (2 species, 10.53% respectively), and Heterotermitinae, Termitogetoninae (1 species, 5.26% respectively). Since this rapid survey is the first termite assemblage representation in Samunsam Wildlife Sanctuary, the preliminary result may serve as the baseline data for termite composition in the area. Therefore, a whole coverage for the area within this sanctuary would definitely increase the number of termite species found in the sanctuary.

  11. Species composition and structure of regenerated and remnant forest patches within an urban landscape

    Treesearch

    Wayne C. Zipperer

    2002-01-01

    Regenerated and remnant forest patches were inventoried in Syracuse, New York, USA to determine differences in structure, species composition, human disturbances, and landscape context. Patches had similar mean stem diameter, total stem density, and total basal areas, but differed with respect to diameter distribution, disturbance regime, landscape context, and...

  12. Estimation of species extinction: what are the consequences when total species number is unknown?

    PubMed

    Chen, Youhua

    2014-12-01

    The species-area relationship (SAR) is known to overestimate species extinction but the underlying mechanisms remain unclear to a great extent. Here, I show that when total species number in an area is unknown, the SAR model exaggerates the estimation of species extinction. It is proposed that to accurately estimate species extinction caused by habitat destruction, one of the principal prerequisites is to accurately total the species numbers presented in the whole study area. One can better evaluate and compare alternative theoretical SAR models on the accurate estimation of species loss only when the exact total species number for the whole area is clear. This presents an opportunity for ecologists to simulate more research on accurately estimating Whittaker's gamma diversity for the purpose of better predicting species loss.

  13. Canopy tree species determine herb layer biomass and species composition on a reclaimed mine spoil heap.

    PubMed

    Rawlik, Mateusz; Kasprowicz, Marek; Jagodziński, Andrzej M; Kaźmierowski, Cezary; Łukowiak, Remigiusz; Grzebisz, Witold

    2018-09-01

    According facilitative models of succession, trees are great forest ecosystem engineers. The strength of tree stand influences on habitat were tested in rather homogenous conditions where heterogeneity of site condition was not an important influence. We hypothesized that canopy composition affects total aboveground vascular herb layer biomass (THB) and species composition of herb layer plant biomass (SCHB) more significantly than primary soil fertility or slope exposure. The study was conducted in 227 randomly selected research plots in seven types of forest stands: pure with Alnus glutinosa, Betula pendula, Pinus sylvestris, Quercus petraea and Robinia pseudoacacia, and mixed with dominance of Acer pseudoplatanus or Betula pendula located on hilltop and northern, eastern, western, and southern slopes on a reclaimed, afforested post-mining spoil heap of the Bełchatów Brown Coal Mine (Poland). Generalized linear models (GLZ) showed that tree stand species were the best predictors of THB. Non-parametric variance tests showed significantly higher (nearly four times) THB under canopies of A. glutinosa, R. pseudoacacia, B. pendula and Q. petraea, compared to the lowest THB found under canopies of P. sylvestris and mixed with A. pseudoplatanus. Redundancy Analysis (RDA) showed that SCHB was significantly differentiated along gradients of light-nutrient herb layer species requirements. RDA and non-parametric variance tests showed that SCHB under canopies of A. glutinosa, R. pseudoacacia and mixed with A. pseudoplatanus had large shares of nitrophilous ruderal species (32%, 31% and 11%, respectively), whereas SCHB under B. pendula, Q. petraea, mixed with B. pendula and P. sylvestris were dominated by light-demanding meadow (49%, 51%, 51% and 36%, respectively) and Poaceae species. The results indicated the dominant role of tree stand composition in habitat-forming processes, and although primary site properties had minor importance, they were also modified by tree stand

  14. Species composition and biomasses of fishes in tropical seagrasses at Groote Eylandt, northern Australia

    NASA Astrophysics Data System (ADS)

    Blaber, S. J. M.; Brewer, D. T.; Salini, J. P.; Kerr, J. D.; Conacher, C.

    1992-12-01

    The species composition and biomasses of fishes in the tropical seagrasses of Groote Eylandt, northern Australia, were studied in 1989 and 1990. A total of 156 species was recorded. Tall dense seagrass, short seagrass and control (no seagrass) sites in different depths were compared. Shallow (<1 m) sites were dominated by small resident species and juveniles of non-resident species, while deeper waters (to 7 m) were dominated by larger species. Species composition was not significantly different between sites, but species diversity ( H) and evenness ( E) were higher in non-vegetated areas. In slightly deeper water (<2 m) species composition was different between habitats and species diversity was highest in tall seagrass and least in open areas. Most species were more abundant in tall seagrass and least abundant in open areas. Most of the larger fishes, including 11 species of sharks, are piscivores, and most move into shallow sea-grass areas at night, irrespective of tide height. Only five species showed abundance patterns related to tide height and there were no significant seasonal patterns of abundance in any of the communities. The biomasses for all sites and sampling methods were mostly from 1 to 2 g m -2, which is low relative to other inshore tropical areas. The possible causes—the characteristics of adjacent habitats (coral reefs and mangroves) and the role of seagrasses in the life cycle of fishes are discussed. It is suggested that habitat structure is a major determinant of the species composition of fish in tropical seagrass areas, primarily because it affects food availability, both for small residents and juveniles, and for visiting predators.

  15. Influence of Tree Species Composition and Community Structure on Carbon Density in a Subtropical Forest

    PubMed Central

    Hu, Yanqiu; Su, Zhiyao; Li, Wenbin; Li, Jingpeng; Ke, Xiandong

    2015-01-01

    We assessed the impact of species composition and stand structure on the spatial variation of forest carbon density using data collected from a 4-ha plot in a subtropical forest in southern China. We found that 1) forest biomass carbon density significantly differed among communities, reflecting a significant effect of community structure and species composition on carbon accumulation; 2) soil organic carbon density increased whereas stand biomass carbon density decreased across communities, indicating that different mechanisms might account for the accumulation of stand biomass carbon and soil organic carbon in the subtropical forest; and 3) a small number of tree individuals of the medium- and large-diameter class contributed predominantly to biomass carbon accumulation in the community, whereas a large number of seedlings and saplings were responsible for a small proportion of the total forest carbon stock. These findings demonstrate that both biomass carbon and soil carbon density in the subtropical forest are sensitive to species composition and community structure, and that heterogeneity in species composition and stand structure should be taken into account to ensure accurate forest carbon accounting. PMID:26317523

  16. Influence of Tree Species Composition and Community Structure on Carbon Density in a Subtropical Forest.

    PubMed

    Hu, Yanqiu; Su, Zhiyao; Li, Wenbin; Li, Jingpeng; Ke, Xiandong

    2015-01-01

    We assessed the impact of species composition and stand structure on the spatial variation of forest carbon density using data collected from a 4-ha plot in a subtropical forest in southern China. We found that 1) forest biomass carbon density significantly differed among communities, reflecting a significant effect of community structure and species composition on carbon accumulation; 2) soil organic carbon density increased whereas stand biomass carbon density decreased across communities, indicating that different mechanisms might account for the accumulation of stand biomass carbon and soil organic carbon in the subtropical forest; and 3) a small number of tree individuals of the medium- and large-diameter class contributed predominantly to biomass carbon accumulation in the community, whereas a large number of seedlings and saplings were responsible for a small proportion of the total forest carbon stock. These findings demonstrate that both biomass carbon and soil carbon density in the subtropical forest are sensitive to species composition and community structure, and that heterogeneity in species composition and stand structure should be taken into account to ensure accurate forest carbon accounting.

  17. Composition and diversity of fish species in seagrass bed ecosystem at Muara Binuangeun, Lebak, Banten

    NASA Astrophysics Data System (ADS)

    Kholis, N.; Patria, M. P.; Soedjiarti, T.

    2017-07-01

    Research of composition and diversity of fish species in seagrass bed ecosystem at Muara Binuangeun, Lebak, Banten, had been conducted in May and November 2015. Catch per Unit of Effort (CPUE) was used as a method with push net and boat net as fishing gear. Fishing was conducted during low tide. Collected samples were preserved with 10 % Formalin Solution and then being identified in the laboratory. In total, 286 fishes were collected from 17 families and 38 species. Moolgarda sp. was the most relative abundant species (17,13 %) and Istiblennius edentulus was a fish species with the highest relative frequency. Diversity index value of seagrass bed ecosystem was 2,973. Different sampling time showed the different composition of fish, in an example of Arothron immaculatus.

  18. Factors associated with long-term species composition in dry tropical forests of Central India

    NASA Astrophysics Data System (ADS)

    Agarwala, M.; DeFries, R. S.; Qureshi, Q.; Jhala, Y. V.

    2016-10-01

    The long-term future of species composition in forests depends on regeneration. Many factors can affect regeneration, including human use, environmental conditions, and species’ traits. This study examines the influence of these factors in a tropical deciduous forest of Central India, which is heavily used by local, forest-dependent residents for livestock grazing, fuel-wood extraction, construction and other livelihood needs. We measure size-class proportions (the ratio of abundance of a species at a site in a higher size class to total abundance in both lower and higher size classes) for 39 tree species across 20 transects at different intensities of human use. The size-class proportions for medium to large trees and for small to medium-sized trees were negatively associated with species that are used for local construction, while size class proportions for saplings to small trees were positively associated with those species that are fire resistant and negatively associated with livestock density. Results indicate that grazing and fire prevent non-fire resistant species from reaching reproductive age, which can alter the long term composition and future availability of species that are important for local use and ecosystem services. Management efforts to reduce fire and forest grazing could reverse these impacts on long-term forest composition.

  19. [Species composition and geographical distribution of threatened fishes in Yunnan Province of Southwest China].

    PubMed

    Zhang, Qian; Zhong, Jin-Xin

    2013-05-01

    Based on the related published papers, and by using Geographic Information System (ArcGIS 9.3), this paper analyzed the species composition and geographical distribution of threatened fishes in Yunnan Province of Southwest China. There were 83 threatened species living in the Province, belonging to 5 orders, 13 families, and 47 genera. Cypriniformes was absolutely dominant, with 64 species, followed by Siluriformes, with 16 species. Cyprinidae fishes had 51 species, accounting for 79.7% of Cypriniformes. The most species of Cyprinid fishes were of Barbinae (14 species), Cyprininae (10 species), and Cultrinae (10 species). The threatened fishes could be divided into two zoogeographical regions, i. e., Tibetan Plateau region and Oriental region, and their species composition and geographical distribution were resulted from the historical evolution adapted to the related environments. Whatever in rivers and in lakes, the Cyprinid fishes were both absolutely dominant, occupying 36.1% and 31.3% of the total, respectively. The Cyprinid fishes in rivers were mostly of endangered species, while those in lakes were mostly of vulnerable species. The factors affecting the threatened fishes in the Province were discussed from the two aspects of geodynamic evolution and present situation.

  20. Changes in the fatty acid composition in bitter Lupinus species depend on the debittering process.

    PubMed

    Curti, Carolina A; Curti, Ramiro N; Bonini, Norberto; Ramón, Adriana N

    2018-10-15

    The evaluation of changes in the fatty acid composition in Lupinus species after the debittering process is crucial to determine their nutritional implications. The aim of this study was to evaluate changes in the fatty acid composition in Lupinus albus and L. mutabilis after the debittering process. Lupinus species showed different fatty acid compositions which changed depending on the debittering process applied. The debittering process changed the monounsaturated and polyunsaturated fatty acids in L. albus, whereas in L. mutabilis it changed the w-6/w-3 ratio. However, the total saturated fatty acid content remained stable in both species after the debittering process. The changes in L. albus were associated with the fatty acid desaturation and a conversion into unsaturated fatty acids, whereas in L. mutabilis with the lipid peroxidation by decreasing the linoleic acid content. Nutritional implications of these changes in the fatty acid composition are discussed. Copyright © 2018. Published by Elsevier Ltd.

  1. Species composition of regeneration after clearcutting Southern Appalachian hardwoods

    Treesearch

    David L. Loftis

    1989-01-01

    Regeneration after clearcutting of Southern Appalachian hardwood stands varies substantially in species composition not only among sites of different quality and previous-stand composition, but also among sites of similar quality and similar previous-stand composition. Severe competition from less desirable species for available growing space is cOllDlon in regenerated...

  2. Total phenolic content, radical scavenging properties, and essential oil composition of Origanum species from different populations.

    PubMed

    Dambolena, José S; Zunino, María P; Lucini, Enrique I; Olmedo, Rubén; Banchio, Erika; Bima, Paula J; Zygadlo, Julio A

    2010-01-27

    The aim of this work was to compare the antiradical activity, total phenol content (TPC), and essential oil composition of Origanum vulgare spp. virens, Origanum x applii, Origanum x majoricum, and O. vulgare spp. vulgare cultivated in Argentina in different localities. The experiment was conducted in the research station of La Consulta (INTA-Mendoza), the research station of Santa Lucia (INTA-San Juan), and Agronomy Faculty of National University of La Pampa, from 2007 to 2008. The composition of the essential oils of oregano populations was independent of cultivation conditions. In total, 39 compounds were identified in essential oils of oregano from Argentina by means of GC-MS. Thymol and trans-sabinene hydrate were the most prominent compounds, followed by gamma-terpinene, terpinen-4-ol, and alpha-terpinene. O. vulgare vulgare is the only Origanum studied which is rich in gamma-terpinene. Among tested oregano, O. x majoricum showed the highest essential oil content, 3.9 mg g(-1) dry matter. The plant extract of O. x majoricum had greater total phenol content values, 19.36 mg/g dry weight, than the rest of oregano studied. To find relationships among TPC, free radical scavenging activity (FRSA), and climate variables, canonical correlations were calculated. The results obtained allow us to conclude that 70% of the TPC and FRSA variability can be explained by the climate variables (R(2) = 0.70; p = 8.3 x 10(-6)), the temperature being the most important climatic variable.

  3. Species richness and composition of epiphytic bryophytes in flooded forests of Caxiuanã National Forest, Eastern Amazon, Brazil.

    PubMed

    Cerqueira, Gabriela R; Ilkiu-Borges, Anna Luiza; Ferreira, Leandro V

    2017-01-01

    This study aimed to compare the richness and composition of the epiphytic bryoflora between várzea and igapó forests in Caxiuanã National Forest, Brazilian Amazon. Bryophytes were collected on 502 phorophytes of Virola surinamensis. Average richness per phorophyte and composition between forests and between dry and rainy periods was tested by two-way analysis and by cluster analysis, respectively. In total, 54 species of 13 families were identified. Richness was greater in igapó forest (44 species) compared to várzea forest (38 species). There was no significant difference in the number of species between the studied periods. Cluster analysis showed the bryoflora composition was different between várzea and igapó, but not between dry and rainy periods. Results did not corroborate the hypothesis that várzea forests harbor higher species richness than igapó forests.

  4. The ghosts of trees past: savanna trees create enduring legacies in plant species composition.

    PubMed

    Stahlheber, Karen A; Crispin, Kimberly L; Anton, Cassidy; D'Antonio, Carla M

    2015-09-01

    Isolated trees in savannas worldwide are known to modify their local environment and interact directly with neighboring plants. Less is known about how related tree species differ in their impacts on surrounding communities, how the effects of trees vary between years, and how composition might change following loss of the tree. To address these knowledge gaps, we explored the following questions: How do savanna trees influence the surrounding composition of herbaceous plants? Is the influence of trees consistent across different species and years? How does this change following the death of the tree? We surveyed herbaceous species composition and environmental attributes surrounding living and dead evergreen and deciduous Quercus trees in California (USA) savannas across several years that differed in their total precipitation. Oak trees of all species created distinct, homogenous understory communities dominated by exotic grasses across several sites. The composition of the low-diversity understory communities showed less interannual variation than open grassland, despite a two-fold difference in precipitation between the driest and wettest year. Vegetation composition was correlated with variation in soil properties, which were strongly affected by trees. Oaks also influenced the communities beyond the edge of the crown, but this depended on site and oak species. Low-diversity understory communities persisted up to 43 years following the death of the tree. A gradual decline in the effect of trees on the physical, environment following death did not result in vegetation becoming more similar to open grassland over time. The presence of long-lasting legacies of past tree crowns highlights the difficulty of assigning control of the current distribution of herbaceous species in grassland to their contemporary environment.

  5. Compositional changes in (iso)flavonoids and estrogenic activity of three edible Lupinus species by germination and Rhizopus-elicitation.

    PubMed

    Aisyah, Siti; Vincken, Jean-Paul; Andini, Silvia; Mardiah, Zahara; Gruppen, Harry

    2016-02-01

    The effects of germination and elicitation on (iso)flavonoid composition of extracts from three edible lupine species (Lupinus luteus, Lupinus albus, Lupinus angustifolius) were determined by RP-UHPLC-MS(n). The total (iso)flavonoid content of lupine increased over 10-fold upon germination, with the total content and composition of isoflavonoids more affected than those of flavonoids. Glycosylated isoflavones were the most predominant compounds found in lupine seedlings. Lesser amounts of isoflavone aglycones, including prenylated ones, were also accumulated. Elicitation with Rhizopus oryzae, in addition to germination, raised the content of isoflavonoids further: the total content of 2'-hydroxygenistein derivatives was increased considerably, without increasing that of genistein derivatives. Elicitation by fungus triggered prenylation of isoflavonoids, especially of the 2'-hydroxygenistein derivatives. The preferred positions of prenylation differed among the three lupine species. The change in isoflavone composition increased the agonistic activity of the extracts towards the human estrogen receptors, whereas no antagonistic activity was observed. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Floral scent composition predicts bee pollination system in five butterfly bush (Buddleja, Scrophulariaceae) species.

    PubMed

    Gong, W-C; Chen, G; Vereecken, N J; Dunn, B L; Ma, Y-P; Sun, W-B

    2015-01-01

    Traditionally, plant-pollinator interactions have been interpreted as pollination syndrome. However, the validity of pollination syndrome has been widely doubted in modern studies of pollination ecology. The pollination ecology of five Asian Buddleja species, B. asiatica, B. crispa, B. forrestii, B. macrostachya and B. myriantha, in the Sino-Himalayan region in Asia, flowering in different local seasons, with scented inflorescences were investigated during 2011 and 2012. These five species exhibited diverse floral traits, with narrow and long corolla tubes and concealed nectar. According to their floral morphology, larger bees and Lepidoptera were expected to be the major pollinators. However, field observations showed that only larger bees (honeybee/bumblebee) were the primary pollinators, ranging from 77.95% to 97.90% of total visits. In this study, floral scents of each species were also analysed using coupled gas chromatography and mass spectrometry (GC-MS). Although the five Buddleja species emitted differentiated floral scent compositions, our results showed that floral scents of the five species are dominated by substances that can serve as attractive signals to bees, including species-specific scent compounds and principal compounds with larger relative amounts. This suggests that floral scent compositions are closely associated with the principal pollinator assemblages in these five species. Therefore, we conclude that floral scent compositions rather than floral morphology traits should be used to interpret plant-pollinator interactions in these Asian Buddleja species. © 2014 German Botanical Society and The Royal Botanical Society of the Netherlands.

  7. Comparison of Free Total Amino Acid Compositions and Their Functional Classifications in 13 Wild Edible Mushrooms.

    PubMed

    Sun, Liping; Liu, Qiuming; Bao, Changjun; Fan, Jian

    2017-02-24

    Thirteen popular wild edible mushroom species in Yunnan Province, Boletus bicolor , Boletus speciosus , Boletus sinicus , Boletus craspedius , Boletus griseus , Boletus ornatipes , Xerocomus , Suillus placidus , Boletinus pinetorus , Tricholoma terreum , Tricholomopsis lividipileata , Termitomyces microcarpus , and Amanita hemibapha , were analyzed for their free amino acid compositions by online pre-column derivazation reversed phase high-performance liquid chromatography (RP-HPLC) analysis. Twenty free amino acids, aspartic acid, glutamic acid, serine, glycine, alanine, praline, cysteine, valine, methionine, phenylalanine, isoleucine, leucine, lysine, histidine, threonine, asparagines, glutamine, arginine, tyrosine, and tryptophan, were determined. The total free amino acid (TAA) contents ranged from 1462.6 mg/100 g in B. craspedius to 13,106.2 mg/100 g in T. microcarpus . The different species showed distinct free amino acid profiles. The ratio of total essential amino acids (EAA) to TAA was 0.13-0.41. All of the analyzed species showed high contents of hydrophobic amino acids, at 33%-54% of TAA. Alanine, cysteine, glutamine, and glutamic acid were among the most abundant amino acids present in all species. The results showed that the analyzed mushrooms possessed significant free amino acid contents, which may be important compounds contributing to the typical mushroom taste, nutritional value, and potent antioxidant properties of these wild edible mushrooms. Furthermore, the principal component analysis (PCA) showed that the accumulative variance contribution rate of the first four principal components reached 94.39%. Cluster analysis revealed EAA composition and content might be an important parameter to separate the mushroom species, and T. microcarpus and A. hemibapha showed remarkable EAA content among the 13 species.

  8. Total leaf crude protein, amino acid composition and elemental content in the USDA-ARS bamboo germplasm collection

    USDA-ARS?s Scientific Manuscript database

    Bamboo shoots and leaves are valuable food sources for both humans and livestock. The USDA-ARS National Plant Germplasm System (NPGS) collections hold 93 bamboo species in 20 genera. Total leaf protein, amino acid composition and elemental content for these important genetic resources had never bee...

  9. Contributions of Dryland Forest (Caatinga) to Species Composition, Richness and Diversity of Drosophilidae.

    PubMed

    Oliveira, G F; Rohde, C; Garcia, A C L; Montes, M A; Valente, V L S

    2016-10-01

    In this study, semi-arid environments were tested to see if they support insect diversity. This was evaluated through the structure of the composition of assemblies of drosophilids in three conservation units placed in three different ecoregions in the dryland forests, Caatinga. This is a unique biome in northeast Brazil, comprising approximately 10% of the country. Species richness was investigated over 2 years during a prolonged drought, considered the worst affliction the Caatinga ecosystem had experienced in the last 50 years. Alpha diversity indices and the ecological similarity between the samples were calculated to determine how the environments drive the composition of Drosophilidae in such semi-arid places. A total of 7352 specimens were sampled. They were classified into 20 species belonging to four genera: Drosophila, Rhinoleucophenga, Scaptodrosophila, and Zaprionus. Drosophila nebulosa Sturtevant (44.5%) and Drosophila cardini Sturtevant (12.5%) were the most abundant species. The occurrences and abundances of all the species differed greatly between sites. These results and other ecological analyses indicate that although placed in the same biome, there are great variability in the drosophilid species and abundance among the three protected and conserved dryland environments.

  10. [Species and size composition of fishes in Barra de Navidad lagoon, Mexican central Pacific].

    PubMed

    González-Sansón, Gaspar; Aguilar-Betancourt, Consuelo; Kosonoy-Aceves, Daniel; Lucano-Ramírez, Gabriela; Ruiz-Ramírez, Salvador; Flores-Ortega, Juan Ramón; Hinojosa-Larios, Angel; de Asís Silva-Bátiz, Francisco

    2014-03-01

    Coastal lagoons are considered important nursery areas for many coastal fishes. Barra de Navidad coastal lagoon (3.76km2) is important for local economy as it supports tourism development and artisanal fisheries. However, the role of this lagoon in the dynamics of coastal fish populations is scarcely known. Thus, the objectives of this research were: to characterize the water of the lagoon and related weather conditions, to develop a systematic list of the ichthyofauna, and to estimate the proportion of juveniles in the total number of individuals captured of most abundant species. Water and fish samples were collected between March 2011 and February 2012. Physical and chemical variables were measured in rainy and dry seasons. Several fishing gears were used including a cast net, beach purse seine and gillnets of four different mesh sizes. Our results showed that the lagoon is most of the time euhaline (salinity 30-40ups), although it can be mixopolyhaline (salinity 18-30ups) during short periods. Chlorophyll and nutrients concentrations suggested eutrophication in the lagoon. Mean water temperature changed seasonally from 24.9 degrees C (April, high tide) to 31.4 degrees C (October, low tide). Considering ichthyofauna species, a total of 36 448 individuals of 92 species were collected, 31 of them adding up to 95% of the total of individuals caught. Dominant species were Anchoa spp. (44.6%), Diapterus peruvianus (10.5%), Eucinostomus currani (8.1%), Cetengraulis mysticetus (7.8%), Mugil curema (5.2%) and Opisthonema libertate (4.5%). The lagoon is an important juvenile habitat for 22 of the 31 most abundant species. These included several species of commercial importance such as snappers (Lutjanus argentiventris, L. colorado and L. novemfasciatus), snook (Centropomus nigrescens) and white mullet (Mugil curema). Other four species seem to use the lagoon mainly as adults. This paper is the first contribution on the composition of estuarine ichthyofauna in Jalisco

  11. Reef flattening effects on total richness and species responses in the Caribbean.

    PubMed

    Newman, Steven P; Meesters, Erik H; Dryden, Charlie S; Williams, Stacey M; Sanchez, Cristina; Mumby, Peter J; Polunin, Nicholas V C

    2015-11-01

    There has been ongoing flattening of Caribbean coral reefs with the loss of habitat having severe implications for these systems. Complexity and its structural components are important to fish species richness and community composition, but little is known about its role for other taxa or species-specific responses. This study reveals the importance of reef habitat complexity and structural components to different taxa of macrofauna, total species richness, and individual coral and fish species in the Caribbean. Species presence and richness of different taxa were visually quantified in one hundred 25-m(2) plots in three marine reserves in the Caribbean. Sampling was evenly distributed across five levels of visually estimated reef complexity, with five structural components also recorded: the number of corals, number of large corals, slope angle, maximum sponge and maximum octocoral height. Taking advantage of natural heterogeneity in structural complexity within a particular coral reef habitat (Orbicella reefs) and discrete environmental envelope, thus minimizing other sources of variability, the relative importance of reef complexity and structural components was quantified for different taxa and individual fish and coral species on Caribbean coral reefs using boosted regression trees (BRTs). Boosted regression tree models performed very well when explaining variability in total (82·3%), coral (80·6%) and fish species richness (77·3%), for which the greatest declines in richness occurred below intermediate reef complexity levels. Complexity accounted for very little of the variability in octocorals, sponges, arthropods, annelids or anemones. BRTs revealed species-specific variability and importance for reef complexity and structural components. Coral and fish species occupancy generally declined at low complexity levels, with the exception of two coral species (Pseudodiploria strigosa and Porites divaricata) and four fish species (Halichoeres bivittatus, H

  12. Closely related intertidal and deep-sea Halomonhystera species have distinct fatty acid compositions

    NASA Astrophysics Data System (ADS)

    Van Campenhout, Jelle; Vanreusel, Ann

    2017-01-01

    The deep-sea free-living nematode Halomonhystera hermesi, dominant in the sulphidic sediments of the Håkon Mosby mud volcano (1280 m, Barent sea slope), is part of the mainly estuarine Halomonhystera disjuncta species complex consisting of five cryptic species (GD1-GD5). Cryptic species have a very similar morphology raising questions on their specific environmental differences. This study analyzed total fatty acid (FA) compositions of H. hermesi and GD1, one of H. hermesi's closest relatives. Additionally, we experimentally investigated the effect of a temperature reduction, salinity increase and sulphide concentrations on GD1's FA composition. Because nematodes are expected to have low amounts of storage FA, total FA compositions most likely reflect FA contents of cellular membranes. The deep-sea nematode H. hermesi had significantly lower saturation levels and increased highly unsaturated fatty acid (HUFAs) proportions due to the presence of docosahexanoic acid (DHA—22:6ω3) and higher eicosapentaenoic acid (EPA—20:5ω3) proportions. HUFAs were absent in H. hermesi's food source indicating the ability and need for this nematode to synthesize HUFAs in a deep-sea environment. Our experimental data revealed that only a decrease in temperature resulted in lower saturated fatty acids proportions, indicating that the FA content of H. hermesi is most likely a response to temperature but not to sulphide concentrations or salinity differences. In experimental nematodes, EPA proportions were low and DHA was absent indicating that other factors than temperature, salinity and sulphides mediate the presence of these HUFAs in H. hermesi.

  13. Estimating Forest Species Composition Using a Multi-Sensor Approach

    NASA Astrophysics Data System (ADS)

    Wolter, P. T.

    2009-12-01

    The magnitude, duration, and frequency of forest disturbance caused by the spruce budworm and forest tent caterpillar has increased over the last century due to a shift in forest species composition linked to historical fire suppression, forest management, and pesticide application that has fostered the increase in dominance of host tree species. Modeling approaches are currently being used to understand and forecast potential management effects in changing insect disturbance trends. However, detailed forest composition data needed for these efforts is often lacking. Here, we used partial least squares (PLS) regression to integrate satellite sensor data from Landsat, Radarsat-1, and PALSAR, as well as pixel-wise forest structure information derived from SPOT-5 sensor data (Wolter et al. 2009), to estimate species-level forest composition of 12 species required for modeling efforts. C-band Radarsat-1 data and L-band PALSAR data were frequently among the strongest predictors of forest composition. Pixel-level forest structure data were more important for estimating conifer rather than hardwood forest composition. The coefficients of determination for species relative basal area (RBA) ranged from 0.57 (white cedar) to 0.94 (maple) with RMSE of 8.88 to 6.44 % RBA, respectively. Receiver operating characteristic (ROC) curves were used to determine the effective lower limits of usefulness of species RBA estimates which ranged from 5.94 % (jack pine) to 39.41 % (black ash). These estimates were then used to produce a dominant forest species map for the study region with an overall accuracy of 78 %. Most notably, this approach facilitated discrimination of aspen from birch as well as spruce and fir from other conifer species which is crucial for the study of forest tent caterpillar and spruce budworm dynamics, respectively, in the Upper Midwest. Thus, use of PLS regression as a data fusion strategy has proven to be an effective tool for regional characterization of forest

  14. [Species composition, distribution and phenological characters of pollen-allergenic plants in Beijing urban area].

    PubMed

    Ouyang, Zhi-Yun; Xin, Jia-Nan; Zheng, Hua; Meng, Xue-Song; Wang, Xiao-Ke

    2007-09-01

    In order to know the species composition, distribution pattern, and phenological characteristics of pollen-allergenic plants in Beijing urban area, an investigation was made, combined with literature survey and experts interviews. The results showed that within the fifth ring of Beijing, there were 99 pollen-allergenic plant species belonging to 32 genera and 19 families, among which, 52 species were native plants, accounting for 52.5% of the total, 26 species were introduced from other regions of China, occupying 26.3% of the total, and 21 species were introduced from foreign countries, being 21.2% of the total. The 32 genera of pollen-allergenic plants in Beijing urban area were mainly North Temperate elements, occupying 40.6% , followed by Cosmopolitan and Pantropic elements. In all functional sections of Beijing urban area, the pollen-allergenic plants were most diversified in urban parks, and had the highest proportion in street tree species. The coverage of herbs with strong pollen allergy was in the order of waste lands > gym centers and institution yards > greenbelts > parks > residential areas > squares. The blooming period of pollen-allergenic arbors in Beijing urban area was concentrated in March and April, while that of pollen-allergenic herbs was from July to September.

  15. The influences of canopy species and topographic variables on understory species diversity and composition in coniferous forests.

    PubMed

    Huo, Hong; Feng, Qi; Su, Yong-hong

    2014-01-01

    Understanding the factors that influence the distribution of understory vegetation is important for biological conservation and forest management. We compared understory species composition by multi-response permutation procedure and indicator species analysis between plots dominated by Qinghai spruce (Picea crassifolia Kom.) and Qilian juniper (Sabina przewalskii Kom.) in coniferous forests of the Qilian Mountains, northwestern China. Understory species composition differed markedly between the forest types. Many heliophilous species were significantly associated with juniper forest, while only one species was indicative of spruce forest. Using constrained ordination and the variation partitioning model, we quantitatively assessed the relative effects of two sets of explanatory variables on understory species composition. The results showed that topographic variables had higher explanatory power than did site conditions for understory plant distributions. However, a large amount of the variation in understory species composition remained unexplained. Forward selection revealed that understory species distributions were primarily affected by elevation and aspect. Juniper forest had higher species richness and α-diversity and lower β-diversity in the herb layer of the understory plant community than spruce forest, suggesting that the former may be more important in maintaining understory biodiversity and community stability in alpine coniferous forest ecosystems.

  16. Community structure, diversity and total biomass of tree species at Kapur dominated forests in Peninsular Malaysia

    NASA Astrophysics Data System (ADS)

    Norafida, N. A. Nik; Nizam, M. S.; Juliana, W. A. Wan

    2013-11-01

    A study was conducted to determine the species composition, diversity and biomass of Kapur (Dryobalanops aromatica Gaertn.f.) dominated forests in Peninsular Malaysia. Three forests were selected in different geographical zones, namely Bukit Bauk Virgin Jungle Reserve (BBVJR), Terengganu, Lesong Forest Reserve (LFR), Pahang and Gunung Belumut Recreational Forest (GBRF), Johor. Thirty plots of 0.1 ha (50 m × 20 m) were established with a total sampling area of 1.0 ha at each forest site. All trees with ≥5 cm diameter at breast height (dbh) were tagged, measured and voucher specimens were collected. Floristic composition in the study plot at BBVJR recorded 55 families, 147 genera and 336 species. In LFR, there were 52 families, 138 genera and 288 species, whereas in GBRF there were 52 families, 132 genera and 271 species. D. aromatica was the most important species in all study plots with the Importance Value Index (IVi) of 17.81%, 23.01% and 16.25% in BBVJR, LFR and GBRF, respectively. Similar trend at family level showed the Dipterocarpaceae was the most important family in each of the areas with the family Importance Value Index (FIVi) of 27.95% (BBVJR), 26.09% (LFR) and 27.16% (GBRF). Shannon diversity index (H'f) and Shannon evenness index (J'f) of trees at BBVJR was 5.02 and 0.86; LFR was 4.63 and 0.82; and GBRF was 4.82 and 0.86, respectively. Sorensen's community similarity coefficient (CCs) showed that tree communities between BBVJR, LFR and GBRF had low similarities with values of 0.3 to 0.4. The highest total biomass estimated was in LFR with a value of 739.44 t/ha, followed by BBVJR at 701.34 t/ha and GBRF at 606.29 t/ha.

  17. Equilibrium distribution of dissolved sulphur species in water at 25°C and 1 atm total pressure

    USGS Publications Warehouse

    Garrels, R.M.; Naeser, C.R.

    1958-01-01

    The Eh-pH diagrams for the equilibrium concentrations in aqueous solution at 25°C of native sulphur and all the various sulphur-containing ions and acids from which the ions are produced have been constructed for systems having a total sulphur concentration of 0.1 molar. The composite of these diagrams indicates that elemental sulphur, H2S, HS− HSO4− and SO4 are the species that predominate in the environments that might be found in nature. This indication is in agreement with the composition of all sulphur-containing minerals.

  18. Nestedness of desert bat assemblages: species composition patterns in insular and terrestrial landscapes.

    PubMed

    Frick, Winifred F; Hayes, John P; Heady, Paul A

    2009-01-01

    Nested patterns of community composition exist when species at depauperate sites are subsets of those occurring at sites with more species. Nested subset analysis provides a framework for analyzing species occurrences to determine non-random patterns in community composition and potentially identify mechanisms that may shape faunal assemblages. We examined nested subset structure of desert bat assemblages on 20 islands in the southern Gulf of California and at 27 sites along the Baja California peninsula coast, the presumable source pool for the insular faunas. Nested structure was analyzed using a conservative null model that accounts for expected variation in species richness and species incidence across sites (fixed row and column totals). Associations of nestedness and island traits, such as size and isolation, as well as species traits related to mobility, were assessed to determine the potential role of differential extinction and immigration abilities as mechanisms of nestedness. Bat faunas were significantly nested in both the insular and terrestrial landscape and island size was significantly correlated with nested structure, such that species on smaller islands tended to be subsets of species on larger islands, suggesting that differential extinction vulnerabilities may be important in shaping insular bat faunas. The role of species mobility and immigration abilities is less clearly associated with nestedness in this system. Nestedness in the terrestrial landscape is likely due to stochastic processes related to random placement of individuals and this may also influence nested patterns on islands, but additional data on abundances will be necessary to distinguish among these potential mechanisms.

  19. Temporal Decay in Timber Species Composition and Value in Amazonian Logging Concessions.

    PubMed

    Richardson, Vanessa A; Peres, Carlos A

    2016-01-01

    Throughout human history, slow-renewal biological resource populations have been predictably overexploited, often to the point of economic extinction. We assess whether and how this has occurred with timber resources in the Brazilian Amazon. The asynchronous advance of industrial-scale logging frontiers has left regional-scale forest landscapes with varying histories of logging. Initial harvests in unlogged forests can be highly selective, targeting slow-growing, high-grade, shade-tolerant hardwood species, while later harvests tend to focus on fast-growing, light-wooded, long-lived pioneer trees. Brazil accounts for 85% of all native neotropical forest roundlog production, and the State of Pará for almost half of all timber production in Brazilian Amazonia, the largest old-growth tropical timber reserve controlled by any country. Yet the degree to which timber harvests beyond the first-cut can be financially profitable or demographically sustainable remains poorly understood. Here, we use data on legally planned logging of ~17.3 million cubic meters of timber across 314 species extracted from 824 authorized harvest areas in private and community-owned forests, 446 of which reported volumetric composition data by timber species. We document patterns of timber extraction by volume, species composition, and monetary value along aging eastern Amazonian logging frontiers, which are then explained on the basis of historical and environmental variables. Generalized linear models indicate that relatively recent logging operations farthest from heavy-traffic roads are the most selective, concentrating gross revenues on few high-value species. We find no evidence that the post-logging timber species composition and total value of forest stands recovers beyond the first-cut, suggesting that the commercially most valuable timber species become predictably rare or economically extinct in old logging frontiers. In avoiding even more destructive land-use patterns, managing

  20. Temporal Decay in Timber Species Composition and Value in Amazonian Logging Concessions

    PubMed Central

    Peres, Carlos A.

    2016-01-01

    Throughout human history, slow-renewal biological resource populations have been predictably overexploited, often to the point of economic extinction. We assess whether and how this has occurred with timber resources in the Brazilian Amazon. The asynchronous advance of industrial-scale logging frontiers has left regional-scale forest landscapes with varying histories of logging. Initial harvests in unlogged forests can be highly selective, targeting slow-growing, high-grade, shade-tolerant hardwood species, while later harvests tend to focus on fast-growing, light-wooded, long-lived pioneer trees. Brazil accounts for 85% of all native neotropical forest roundlog production, and the State of Pará for almost half of all timber production in Brazilian Amazonia, the largest old-growth tropical timber reserve controlled by any country. Yet the degree to which timber harvests beyond the first-cut can be financially profitable or demographically sustainable remains poorly understood. Here, we use data on legally planned logging of ~17.3 million cubic meters of timber across 314 species extracted from 824 authorized harvest areas in private and community-owned forests, 446 of which reported volumetric composition data by timber species. We document patterns of timber extraction by volume, species composition, and monetary value along aging eastern Amazonian logging frontiers, which are then explained on the basis of historical and environmental variables. Generalized linear models indicate that relatively recent logging operations farthest from heavy-traffic roads are the most selective, concentrating gross revenues on few high-value species. We find no evidence that the post-logging timber species composition and total value of forest stands recovers beyond the first-cut, suggesting that the commercially most valuable timber species become predictably rare or economically extinct in old logging frontiers. In avoiding even more destructive land-use patterns, managing

  1. Inference methods for spatial variation in species richness and community composition when not all species are detected

    USGS Publications Warehouse

    Nichols, J.D.; Boulinier, T.; Hines, J.E.; Pollock, K.H.; Sauer, J.R.

    1998-01-01

    Inferences about spatial variation in species richness and community composition are important both to ecological hypotheses about the structure and function of communities and to community-level conservation and management. Few sampling programs for animal communities provide censuses, and usually some species present. We present estimators useful for drawing inferences about comparative species richness and composition between different sampling locations when not all species are detected in sampling efforts. Based on capture-recapture models using the robust design, our methods estimate relative species richness, proportion of species in one location that are also found in another, and number of species found in one location but not in another. The methods use data on the presence or absence of each species at different sampling occasions (or locations) to estimate the number of species not detected at any occasions (or locations). This approach permits estimation of the number of species in the sampled community and in subsets of the community useful for estimating the fraction of species shared by two communities. We provide an illustration of our estimation methods by comparing bird species richness and composition in two locations sampled by routes of the North American Breeding Bird Survey. In this example analysis, the two locations (an associated bird communities) represented different levels of urbanization. Estimates of relative richness, proportion of shared species, and number of species present on one route but not the other indicated that the route with the smaller fraction of urban area had greater richness and a larer number of species that were not found on the more urban route than vice versa. We developed a software package, COMDYN, for computing estimates based on the methods. Because these estimation methods explicitly deal with sampling in which not all species are detected, we recommend their use for addressing questions about species

  2. Lipid Molecular Species Composition in Developing Soybean Cotyledons 1

    PubMed Central

    Wilson, Richard F.; Rinne, Robert W.

    1978-01-01

    The fatty acid composition of triglyceride and phospholipids in developing soybean cotyledons (Glycine max L., var. “Harosoy 63”) was analyzed at several stages of growth between 30 and 70 days after flowering. Changes observed in fatty acid composition within each lipid class were related to the levels of lipid molecular species present in the oil. Thirteen molecular species of triglyceride were identified in developing cotyledons, however three of these groups: trilinolenic, dilinolenic-monolinoleic, and linolenic-linoleic-oleic triglycerides, were not found in the mature seed. In immature cotyledons, trioleic and trilinoleic triglycerides accounted for 50% of the structures found; the level of these molecules decreased to 24.9% in the mature seed. The dilinoleic-monolinolenic triglycerides increased from 0.4 to 23.4% during cotyledon development. Changes in triglyceride composition were compared to the levels of molecular species for each phospholipid class. Dilinoleic and monosaturated monolinoleic phospholipid species were dominant in all phospholipid classes throughout development. PMID:16660395

  3. [Species composition and distribution of medical mollusca in Shanghai City].

    PubMed

    Guo, Yun-hai; Lv, Shan; Gu, Wen-biao; Liu, He-xiang; Wu, Ying; Zhang, Yi

    2015-02-01

    To investigate the species diversity and distribution of medical mollusca in Shanghai City. From August 2012 to October 2013, all kinds of habitats in 8 districts and counties in Shanghai City, namely Jiading, Qingpu, Baoshan, Minhang, Songjiang, Jinshan, Chongming, Pudong, were selected for the field survey according to the distribution characteristics of the river system, and all the specimens of medical mollusca in the investigation sites were collected and classified by morphological identification. Meanwhile, the species composition, habitats as well as the fauna of the medical mollusca collected were analyzed. A total of 5,211 specimens were collected, which belonged to 2 classes, 14 families, 18 genera and 25 species, including Oncomelania hupensis hupensis, Pomacea canaliculata, Parafossarulus striatulus, Alocinma longicornis, Physa acuta, Galba pervia, Hippeutis cantori, etc. The species numbers of medical mollusca in Chongming, Jinshan, Pudong new area and Qingpu districts (counties) were 22, 22, 21 and 20, respectively, which were more than those of other areas. The habitat analysis suggested that the species numbers in the river and wetland were the most, both of which were 14 species. The main faunas of the medical mollusca in Shanghai were the cosmopolitan and oriental species. The freshwater gastropod species are paucity in Shanghai City, but almost of them can be served as the intermediate hosts of certain parasites to transmit snail-related parasitic diseases, so the surveillance of medical mollusca should be strengthened.

  4. Species composition of Bromeliaceae and their distribution at the Massambaba restinga in Arraial do Cabo, Rio de Janeiro, Brazil.

    PubMed

    Rocha-Pessôa, T C; Nunes-Freitas, A F; Cogliatti-Carvalho, L; Rocha, C F D

    2008-05-01

    We studied some ecological parameters such as richness, abundance, density, biomass and variation in species composition in four vegetation zones and in a zone with anthropic disturbance in the Massambaba Restinga in Arraial do Cabo, Rio de Janeiro State. We sampled 100 plots of 100 m(2) (10 x 10 m) recording the bromeliad species and their abundance. We found a total of seven bromeliad species, with Vriesea neoglutinosa (5647 ramets) and Tillandsia stricta (1277 ramets) being the most abundant. The vegetation zone called Clusia shrubs had the highest richness (S = 5) and density (6360 ramets.ha(-1)) of bromeliads. The differences found in abundance and variation in species composition among vegetation zones seems to be related to the vegetation structure of each zone.

  5. Positional-Species Composition of Diacylglycerol Acetates from Mature Euonymus Seeds.

    PubMed

    Sidorov, Roman A; Pchelkin, Vasily P; Zhukov, Anatoly V; Tsydendambaev, Vladimir D

    2016-06-01

    The positional-species composition (PSC) of 3-acetyl-1,2-diacyl-sn-glycerols (AcDAGs) from the seeds of mature fruits of 14 species of the genus Euonymus L. was established. The residues of six major fatty acids (FAs), palmitic (P), stearic (St), hexadecenoic (H), octadecenoic (O), linoleic (L), and linolenic (Ln), were present in the AcDAGs. Here, we demonstrated that the profile of PSC of AcDAGs could serve as chemotaxonomic factor to divide euonymus species studied here into groups which completely correlate with the present day systematic of the genus. In particular, the Euonymus section greatly exceeded other sections of the Euonymus subgenus as well as the Kalonymus one in the total levels of AcDAGs positional species having one and two O residues and was characterized by significantly lesser concentrations of species with one and two L residues. Moreover, in seed, AcDAGs of almost all Euonymus species EFL values were slightly higher than EFO ones, but all EFL and EFO values were higher than 1.0, and therefore, it can be concluded that both FAs mainly esterified sn-2-position of the glycerol moiety and saturated FAs residues were always virtually absent in the sn-2 position of Euonymus seed AcDAGs, as it is also the case in nearly all TAGs molecules of plant origin. © 2016 Verlag Helvetica Chimica Acta AG, Zürich.

  6. [Effects of tree species transition on soil microbial community composition and functions in subtropical China].

    PubMed

    Ding, Guo Chang; Wang, Xiao Hua; Yang, Qi Fan; Lin, Qun Xing; Huang, Zhi Qun

    2017-11-01

    We employed a comparative study to examine the effects of tree species transition on soil microbial biomass, community composition and enzymes activities under Cunninghamia lanceolata (Lamb.) Hook, Eucalyptus grandis and a N-fixing species, Acacia melanoxylon in subtropical China. Results showed that the effect of tree species on soil microbial community and enzymes activities was significant only in the 0-10 cm soil layer. Reforestation with N-fixing species A. melanoxylon on the C. lanceolata harvest site significantly increased the total phospholipid fatty acid (PLFA), fungal PLFAs, Gram-positive bacterial PLFAs, Gram-negative bacterial PLFAs and actinomycetes biomasses in the 0-10 cm soil layer. The principal component analysis (PCA) showed that the soil microbial community composition in A. melanoxylon soil differed significantly from that in C. lanceolata and E. grandis soils. N-fixing species (A. melanoxylon) significantly enhanced the percent abundance of Gram-positive bacteria, Gram-negative bacteria and actinomycetes. Activities of cellobiohydrolase, N-acetyl-β-d-glucosaminidase and acid phosphatase were significantly higher under A. melanoxylon than under C. lanceolata and E. grandis plantations. Our results suggested that reforestation with N-fixing species, A. melanoxylon on C. lanceolata harvest site could increase soil microbial biomass, enzyme activities and soil organic matter content.

  7. Environmental gradients explain species richness and community composition of coastal breeding birds in the Baltic Sea.

    PubMed

    Nord, Maria; Forslund, Pär

    2015-01-01

    Scientifically-based systematic conservation planning for reserve design requires knowledge of species richness patterns and how these are related to environmental gradients. In this study, we explore a large inventory of coastal breeding birds, in total 48 species, sampled in 4646 1 km2 squares which covered a large archipelago in the Baltic Sea on the east coast of Sweden. We analysed how species richness (α diversity) and community composition (β diversity) of two groups of coastal breeding birds (specialists, i.e. obligate coastal breeders; generalists, i.e. facultative coastal breeders) were affected by distance to open sea, land area, shoreline length and archipelago width. The total number of species per square increased with increasing shoreline length, but increasing land area counteracted this effect in specialists. The number of specialist bird species per square increased with decreasing distance to open sea, while the opposite was true for the generalists. Differences in community composition between squares were associated with differences in land area and distance to open sea, both when considering all species pooled and each group separately. Fourteen species were nationally red-listed, and showed similar relationships to the environmental gradients as did all species, specialists and generalists. We suggest that availability of suitable breeding habitats, and probably also proximity to feeding areas, explain much of the observed spatial distributions of coastal birds in this study. Our findings have important implications for systematic conservation planning of coastal breeding birds. In particular, we provide information on where coastal breeding birds occur and which environments they seem to prefer. Small land areas with long shorelines are highly valuable both in general and for red-listed species. Thus, such areas should be prioritized for protection against human disturbance and used by management in reserve selection.

  8. Environmental Gradients Explain Species Richness and Community Composition of Coastal Breeding Birds in the Baltic Sea

    PubMed Central

    Nord, Maria; Forslund, Pär

    2015-01-01

    Scientifically-based systematic conservation planning for reserve design requires knowledge of species richness patterns and how these are related to environmental gradients. In this study, we explore a large inventory of coastal breeding birds, in total 48 species, sampled in 4646 1 km2 squares which covered a large archipelago in the Baltic Sea on the east coast of Sweden. We analysed how species richness (α diversity) and community composition (β diversity) of two groups of coastal breeding birds (specialists, i.e. obligate coastal breeders; generalists, i.e. facultative coastal breeders) were affected by distance to open sea, land area, shoreline length and archipelago width. The total number of species per square increased with increasing shoreline length, but increasing land area counteracted this effect in specialists. The number of specialist bird species per square increased with decreasing distance to open sea, while the opposite was true for the generalists. Differences in community composition between squares were associated with differences in land area and distance to open sea, both when considering all species pooled and each group separately. Fourteen species were nationally red-listed, and showed similar relationships to the environmental gradients as did all species, specialists and generalists. We suggest that availability of suitable breeding habitats, and probably also proximity to feeding areas, explain much of the observed spatial distributions of coastal birds in this study. Our findings have important implications for systematic conservation planning of coastal breeding birds. In particular, we provide information on where coastal breeding birds occur and which environments they seem to prefer. Small land areas with long shorelines are highly valuable both in general and for red-listed species. Thus, such areas should be prioritized for protection against human disturbance and used by management in reserve selection. PMID:25714432

  9. Historical factors shaped species diversity and composition of Salix in eastern Asia.

    PubMed

    Wang, Qinggang; Su, Xiangyan; Shrestha, Nawal; Liu, Yunpeng; Wang, Siyang; Xu, Xiaoting; Wang, Zhiheng

    2017-02-08

    Ambient energy, niche conservatism, historical climate stability and habitat heterogeneity hypothesis have been proposed to explain the broad-scale species diversity patterns and species compositions, while their relative importance have been controversial. Here, we assessed the relative contributions of contemporary climate, historical climate changes and habitat heterogeneity in shaping Salix species diversity and species composition in whole eastern Asia as well as mountains and lowlands using linear regressions and distance-based redundancy analyses, respectively. Salix diversity was negatively related with mean annual temperature. Habitat heterogeneity was more important than contemporary climate in shaping Salix diversity patterns, and their relative contributions were different in mountains and lowlands. In contrast, the species composition was strongly influenced by contemporary climate and historical climate change than habitat heterogeneity, and their relative contributions were nearly the same both in mountains and lowlands. Our findings supported niche conservatism and habitat heterogeneity hypotheses, but did not support ambient energy and historical climate stability hypotheses. The diversity pattern and species composition of Salix could not be well-explained by any single hypothesis tested, suggesting that other factors such as disturbance history and diversification rate may be also important in shaping the diversity pattern and composition of Salix species.

  10. Historical factors shaped species diversity and composition of Salix in eastern Asia

    PubMed Central

    Wang, Qinggang; Su, Xiangyan; Shrestha, Nawal; Liu, Yunpeng; Wang, Siyang; Xu, Xiaoting; Wang, Zhiheng

    2017-01-01

    Ambient energy, niche conservatism, historical climate stability and habitat heterogeneity hypothesis have been proposed to explain the broad-scale species diversity patterns and species compositions, while their relative importance have been controversial. Here, we assessed the relative contributions of contemporary climate, historical climate changes and habitat heterogeneity in shaping Salix species diversity and species composition in whole eastern Asia as well as mountains and lowlands using linear regressions and distance-based redundancy analyses, respectively. Salix diversity was negatively related with mean annual temperature. Habitat heterogeneity was more important than contemporary climate in shaping Salix diversity patterns, and their relative contributions were different in mountains and lowlands. In contrast, the species composition was strongly influenced by contemporary climate and historical climate change than habitat heterogeneity, and their relative contributions were nearly the same both in mountains and lowlands. Our findings supported niche conservatism and habitat heterogeneity hypotheses, but did not support ambient energy and historical climate stability hypotheses. The diversity pattern and species composition of Salix could not be well-explained by any single hypothesis tested, suggesting that other factors such as disturbance history and diversification rate may be also important in shaping the diversity pattern and composition of Salix species. PMID:28176816

  11. Study of Culex tritaeniorhynchus and species composition of mosquitoes in a rice field in Greece.

    PubMed

    Lytra, Ioanna; Emmanouel, Nikolaos

    2014-06-01

    Mosquito species composition and seasonal abundance were studied in a rice field in western Greece over a three-year period (2009-2011). A total of 11,716 larvae and pupae of mosquitoes were recorded, representing seven species, namely Aedes caspius (Pallas), Anopheles hyrcanus (Pallas), Anopheles sacharovi Favre, Culex theileri Theobald, Culex tritaeniorhynchus Giles, Culex pipiens Linnaeus, Uranotaenia unguiculata Edwards and belonging to four genera. Cx. tritaeniorhynchus constituted the most abundant species. It is the second recorded occurrence of this species in Greece, but the first time that a high population of this mosquito species is recorded in the country. In all three years, the total population density of mosquitoes was found to be higher in early August. The number of immatures of all species was found higher in 2009 and 2010 than in 2011, as well was that of the Cx. tritaeniorhynchus adults derived from the rearing of the collected immatures. This regularity is probably due to the lack of water in the rice field in early August 2011. Cx. tritaeniorhynchus was found to be the most abundant species after the rearing of immatures representing 85.1%, 93.5% and 96.1% of the total number of the mosquito adults in 2009, 2010 and 2011, respectively. The rice culturing practices may have affected the seasonal occurrence of mosquito immatures in all of the study years. Copyright © 2014 Elsevier B.V. All rights reserved.

  12. Effects of Re-vegetation on Herbaceous Species Composition and Biological Soil Crusts Development in a Coal Mine Dumping Site

    NASA Astrophysics Data System (ADS)

    Zhao, Yang; Zhang, Peng; Hu, Yigang; Huang, Lei

    2016-02-01

    Despite the critical roles of plant species' diversity and biological soil crusts (BSCs) in arid and semi-arid ecosystems, the restoration of the diversity of herbaceous species and BSCs are rarely discussed during the process of vegetation restoration of anthropogenically damaged areas in these regions. In this study, the herbaceous plant species composition, along with the BSCs coverage and thicknesses, was investigated at six different re-vegetation type sites, and the natural vegetation site of the Heidaigou open pit coal mine in China's Inner Mongolia Autonomous Region was used as a reference. The highest total species richness (16), as well as the species richness (4.4), occurred in the Tree and Herbaceous vegetation type site. The species composition similarities between the restored sites and the reference site were shown to be very low, and ranged from 0.09 to 0.42. Also, among the restored sites, the similarities of the species were fairly high and similar, and ranged from 0.45 to 0.93. The density and height of the re-vegetated woody plants were significantly correlated with the indexes of the diversity of the species. The Shrub vegetation type site showed the greatest total coverage (80 %) of BSCs and algae crust coverage (48 %). The Shrub and Herbaceous type had the greatest thicknesses of BSCs, with as much as 3.06 mm observed, which was followed by 2.64 mm for the Shrub type. There was a significant correlation observed between the coverage of the total BSCs, and the total vegetation and herbaceous vegetation coverage, as well as between the algae crust coverage and the herbaceous vegetation coverage. It has been suggested that the re-vegetated dwarf woody plant species (such as shrubs and semi-shrubs) should be chosen for the optimal methods of the restoration of herbaceous species diversity at dumping sites, and these should be planted with low density. Furthermore, the effects of vegetation coverage on the colonization and development the BSCs

  13. Mosquito species composition and phenology (Diptera, Culicidae) in two German zoological gardens imply different risks of mosquito-borne pathogen transmission.

    PubMed

    Heym, Eva C; Kampen, Helge; Walther, Doreen

    2018-06-01

    Due to their large diversity of potential blood hosts, breeding habitats, and resting sites, zoological gardens represent highly interesting places to study mosquito ecology. In order to better assess the risk of mosquito-borne disease-agent transmission in zoos, potential vector species must be known, as well as the communities in which they occur. For this reason, species composition and dynamics were examined in 2016 in two zoological gardens in Germany. Using different methods for mosquito sampling, a total of 2,257 specimens belonging to 20 taxa were collected. Species spectra depended on the collection method but generally differed between the two zoos, while species compositions and relative abundances varied seasonally in both of them. As both sampled zoos were located in the same climatic region and potential breeding sites within the zoos were similar, the differences in mosquito compositions are attributed to immigration of specimens from surrounding landscapes, although the different sizes of the zoos and the different blood host populations available probably also have an impact. Based on the differences in species composition and the various biological characteristics of the species, the risk of certain pathogens to be transmitted must also be expected to differ between the zoos. © 2018 The Society for Vector Ecology.

  14. Effects of processing on proximate and fatty acid compositions of six commercial sea cucumber species of Sri Lanka.

    PubMed

    Nishanthan, G; Kumara, P A D A; de Croos, M D S T; Prasada, D V P; Dissanayake, D C T

    2018-05-01

    Processing and its impacts on proximate composition and fatty acid profile of six sea cucumber species; Bohadschia marmorata, Stichopus chloronotus, Holothuria spinifera, Thelenota anax, Holothuria scabra and Bohadschia sp. 1 collected from the northwest coast of Sri Lanka were analyzed. Sea cucumbers are processed into bêche - de - mer by both domestic and industrial level processors following the similar steps of cleaning, evisceration, first boiling, salting, second boiling and drying. However, domestically processed bêche - de - mer always reported a higher percentage of moisture, crude ash, crude fat and lower percentage of crude protein than industrially processed products. Although processing resulted in a significant reduction of total SFA and MUFA in fresh individuals of most of these species, total PUFA increased significantly in processed individuals excluding Bohadschia species. Palmitic acid was found to be the most dominant fatty acid in all these species followed by eicosapentaenoic acid, which showed a significant increase in processed products, except Bohadschia sp. 1. Total MUFA were higher than total SFA in all sea cucumber species having exceptions in Bohadchia sp.1 and fresh S. chloronotus. These findings will make a significant contribution to fill the gaps in existing information as no any previous information is available for species like H. spinifera and S. chloronotus .

  15. DNA barcoding of shark meats identify species composition and CITES-listed species from the markets in Taiwan.

    PubMed

    Liu, Shang-Yin Vanson; Chan, Chia-Ling Carynn; Lin, Oceana; Hu, Chieh-Shen; Chen, Chaolun Allen

    2013-01-01

    An increasing awareness of the vulnerability of sharks to exploitation by shark finning has contributed to a growing concern about an unsustainable shark fishery. Taiwan's fleet has the 4th largest shark catch in the world, accounting for almost 6% of the global figures. Revealing the diversity of sharks consumed by Taiwanese is important in designing conservation plans. However, fins make up less than 5% of the total body weight of a shark, and their bodies are sold as filets in the market, making it difficult or impossible to identify species using morphological traits. In the present study, we adopted a DNA barcoding technique using a 391-bp fragment of the mitochondrial cytochrome oxidase I (COI) gene to examine the diversity of shark filets and fins collected from markets and restaurants island-wide in Taiwan. Amongst the 548 tissue samples collected and sequenced, 20 major clusters were apparent by phylogenetic analyses, each of them containing individuals belonging to the same species (most with more than 95% bootstrap values), corresponding to 20 species of sharks. Additionally, Alopias pelagicus, Carcharhinus falciformis, Isurus oxyrinchus, and Prionace glauca consisted of 80% of the samples we collected, indicating that these species might be heavily consumed in Taiwan. Approximately 5% of the tissue samples used in this study were identified as species listed in CITES Appendix II, including two species of Sphyrna, C. longimanus and Carcharodon carcharias. DNA barcoding provides an alternative method for understanding shark species composition when species-specific data is unavailable. Considering the global population decline, stock assessments of Appendix II species and highly consumed species are needed to accomplish the ultimate goal of shark conservation.

  16. Seed Carotenoid and Tocochromanol Composition of Wild Fabaceae Species Is Shaped by Phylogeny and Ecological Factors

    PubMed Central

    Fernández-Marín, Beatriz; Míguez, Fátima; Méndez-Fernández, Leire; Agut, Agustí; Becerril, José M.; García-Plazaola, José I.; Kranner, Ilse; Colville, Louise

    2017-01-01

    Carotenoids distribution and function in seeds have been very scarcely studied, notwithstanding their pivotal roles in plants that include photosynthesis and phytohormone synthesis, pigmentation, membrane stabilization and antioxidant activity. Their relationship with tocochromanols, whose critical role in maintaining seed viability has already been evidenced, and with chlorophylls, whose retention in mature seed is thought to have negative effects on storability, remain also unexplored. Here, we aimed at elucidating seed carotenoids relationship with tocochromanols and chlorophylls with regard to phylogenetic and ecological traits and at understanding their changes during germination. The composition and distribution of carotenoids were investigated in seeds of a wide range of wild species across the Fabaceae (the second-most economically important family after the Poaceae). Photosynthetic pigments and tocochromanols were analyzed by HPLC in mature dry seeds of 50 species representative of 5 subfamilies within the Fabaceae (including taxa that represent all continents, biomes and life forms within the family) and at key timepoints during seedling establishment in three species representative of distinct clades. Total-carotenoids content positively correlated with tocopherols in the basal subfamilies Detarioideae, Cercidoideae, and Dialioideae, and with chlorophylls in the Papilionoideae. Papilionoideae lacked tocotrienols and had the highest total-carotenoids, chlorophyll and γ-tocopherol contents. Interestingly, lutein epoxide was present in 72% of the species including several herbs from different subfamilies. Overall, species original from temperate biomes presented higher carotenoids and lower tocochromanols levels than those from tropical biomes. Also shrub species showed higher carotenoids content than herbs and trees. During germination, total content of photosynthetic pigments increased in parallel to changes in relative abundance of carotenoids

  17. Long-term changes of tree species composition and distribution in Korean mountain forests

    NASA Astrophysics Data System (ADS)

    Lee, Boknam; Lee, Hoontaek; Cho, Sunhee; Yoon, Jongguk; Park, Jongyoung; Kim, Hyun Seok

    2017-04-01

    Long-term changes in the abundance and distribution of tree species in the temperate forests of South Korea remain poorly understood. We investigated how tree species composition and stand distribution change across temperate mountainous forests using the species composition and DBH size collected over the past 15 years (1998-2012) across 130 permanent forest plots of 0.1 ha in Jiri and Baegun mountains in South Korea. The overall net change of tree communities over the years showed positive in terms of stand density, richness, diversity, and evenness. At the species level, the change of relative species composition has been led by intermediate and shade-tolerant species, such as Quercus mongolica, Carpinus laxiflora, Quercus serrate, Quercus variabilis, Styrax japonicus, Lindera erythrocarpa, and Pinus densiflora and was categorized into five species communities, representing gradual increase or decrease, establishment, extinction, fluctuation of species population. At the community level, the change in species composition appeared to have consistent and directional patterns of increase in the annual rate of change in the mean species traits including species density, pole growth rate, adult growth rate, and adult stature. Based on the additive models, the distribution of species diversity was significantly related to topographical variables including elevation, latitude, longitude, slope, topographic wetness index, and curvature where elevation was the most significant driver, followed by latitude and longitude. However, the change in distribution of species diversity was only significantly influenced by latitude and longitude. This is the first study to reveal the long-term dynamics of change in tree species composition and distribution, which are important to broaden our understanding of temperate mountainous forest ecosystem in South Korea.

  18. Healthy n-6/n-3 fatty acid composition from five European game meat species remains after cooking.

    PubMed

    Valencak, Teresa G; Gamsjäger, Lisa; Ohrnberger, Sarah; Culbert, Nicole J; Ruf, Thomas

    2015-06-27

    Intensive farming of livestock along with recent food scandals and consumer deception have increased awareness about risks for human nutrition. In parallel, the demand for meat obtained under more natural conditions from animals that can freely forage has largely increased. Interestingly, the consumption of game meat has not become more common despite its excellent quality and content of polyunsaturated fatty acids (PUFAs). We addressed the question if game meat fatty acid composition is modified through kitchen preparation. By analysing muscle fatty acid (FA) composition (polar and total lipids) of five European game species in a raw and a processed state, we aimed to quantify the proportion of PUFA that are oxidised and hydrogenated during processing. All game meat species originated from local hunters and free-living individuals. To mimic a realistic situation a professional chef prepared the meat samples with gentle use of heat in a standardised way. Expectedly, the overall content of polyunsaturated fatty acids declined during the cooking process but the decrease size was <5% and the nutritiously most important n-3/n-6 ratio was not affected by processing (F1,54 = 0.46; p = 0.5). Generally, our samples contained species-specific high PUFA and n-3 FA contents but we point out that differentiating between species is necessary. Game meat thus provides a healthy meat source, as cooking does not substantially alter its favourable fatty acid composition. Further research is needed to elucidate species-specific differences and the role of habitat quality and locomotion for tissue composition.

  19. Total free radical species and oxidation equivalent in polluted air.

    PubMed

    Wang, Guoying; Jia, Shiming; Niu, Xiuli; Tian, Haoqi; Liu, Yanrong; Chen, Xuefu; Li, Lan; Zhang, Yuanhang; Shi, Gaofeng

    2017-12-31

    Free radicals are the most important chemical intermediate or agent of the atmosphere and influenced by thousands of reactants. The free radicals determine the oxidizing power of the polluted air. Various gases present in smog or haze are oxidants and induce organ and cellular damage via generation of free radical species. At present, however, the high variability of total free radicals in polluted air has prevented the detection of possible trends or distributions in the concentration of those species. The total free radicals are a kind of contaminants with colorless, tasteless characteristics, and almost imperceptible by human body. Here we present total free radical detection and distribution characteristics, and analyze the effects of total free radicals in polluted air on human health. We find that the total free radical values can be described by not only a linear dependence on ozone at higher temperature period, but also a linear delay dependence on particulate matter at lower temperature period throughout the measurement period. The total free radical species distribution is decrease from west to east in Lanzhou, which closely related to the distribution of the air pollutants. The total free radical oxidation capacity in polluted air roughly matches the effects of tobacco smoke produced by the incomplete combustion of a controlled amount of tobacco in a smoke chamber. A relatively unsophisticated chromatographic fingerprint similarity is used for indicating preliminarily the effect of total free radicals in polluted air on human health. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Modern tree species composition reflects ancient Maya "forest gardens" in northwest Belize.

    PubMed

    Ross, Nanci J

    2011-01-01

    Ecology and ethnobotany were integrated to assess the impact of ancient Maya tree-dominated home gardens (i.e., "forest gardens"), which contained a diversity of tree species used for daily household needs, on the modern tree species composition of a Mesoamerican forest. Researchers have argued that the ubiquity of these ancient gardens throughout Mesoamerica led to the dominance of species useful to Maya in the contemporary forest, but this pattern may be localized depending on ancient land use. The tested hypothesis was that species composition would be significantly different between areas of dense ancient residential structures (high density) and areas of little or no ancient settlement (low density). Sixty-three 400-m2 plots (31 high density and 32 low density) were censused around the El Pilar Archaeological Reserve in northwestern Belize. Species composition was significantly different, with higher abundances of commonly utilized "forest garden" species still persisting in high-density forest areas despite centuries of abandonment. Subsequent edaphic analyses only explained 5% of the species composition differences. This research provides data on the long-term impacts of Maya forests gardens for use in development of future conservation models. For Mesoamerican conservation programs to work, we must understand the complex ecological and social interactions within an ecosystem that developed in intimate association with humans.

  1. Survey of the total fatty acid and triacylglycerol composition and content of 30 duckweed species and cloning of a Δ6-desaturase responsible for the production of γ-linolenic and stearidonic acids in Lemna gibba

    PubMed Central

    2013-01-01

    Background Duckweeds, i.e., members of the Lemnoideae family, are amongst the smallest aquatic flowering plants. Their high growth rate, aquatic habit and suitability for bio-remediation make them strong candidates for biomass production. Duckweeds have been studied for their potential as feedstocks for bioethanol production; however, less is known about their ability to accumulate reduced carbon as fatty acids (FA) and oil. Results Total FA profiles of thirty duckweed species were analysed to assess the natural diversity within the Lemnoideae. Total FA content varied between 4.6% and 14.2% of dry weight whereas triacylglycerol (TAG) levels varied between 0.02% and 0.15% of dry weight. Three FA, 16:0 (palmitic), 18:2Δ9,12 (Linoleic acid, or LN) and 18:3Δ9,12,15 (α-linolenic acid, or ALA) comprise more than 80% of total duckweed FA. Seven Lemna and two Wolffiela species also accumulate polyunsaturated FA containing Δ6-double bonds, i.e., GLA and SDA. Relative to total FA, TAG is enriched in saturated FA and deficient in polyunsaturated FA, and only five Lemna species accumulate Δ6-FA in their TAG. A putative Δ6-desaturase designated LgDes, with homology to a family of front-end Δ6-FA and Δ8-spingolipid desaturases, was identified in the assembled DNA sequence of Lemna gibba. Expression of a synthetic LgDes gene in Nicotiana benthamiana resulted in the accumulation of GLA and SDA, confirming it specifies a Δ6-desaturase. Conclusions Total accumulation of FA varies three-fold across the 30 species of Lemnoideae surveyed. Nine species contain GLA and SDA which are synthesized by a Δ6 front-end desaturase, but FA composition is otherwise similar. TAG accumulates up to 0.15% of total dry weight, comparable to levels found in the leaves of terrestrial plants. Polyunsaturated FA is underrepresented in TAG, and the Δ6-FA GLA and SDA are found in the TAG of only five of the nine Lemna species that produce them. When present, GLA is enriched and SDA diminished

  2. Chemical composition, mineral content and amino acid and lipid profiles in bones from various fish species.

    PubMed

    Toppe, Jogeir; Albrektsen, Sissel; Hope, Britt; Aksnes, Anders

    2007-03-01

    The chemical composition, content of minerals and the profiles of amino acids and fatty acids were analyzed in fish bones from eight different species of fish. Fish bones varied significantly in chemical composition. The main difference was lipid content ranging from 23 g/kg in cod (Gadus morhua) to 509 g/kg in mackerel (Scomber scombrus). In general fatty fish species showed higher lipid levels in the bones compared to lean fish species. Similarly, lower levels of protein and ash were observed in bones from fatty fish species. Protein levels differed from 363 g/kg lipid free dry matter (dm) to 568 g/kg lipid free dm with a concomitant inverse difference in ash content. Ash to protein ratio differed from 0.78 to 1.71 with the lowest level in fish that naturally have highest swimming and physical activity. Saithe (Pollachius virens) and salmon (Salmo salar) were found to be significantly different in the levels of lipid, protein and ash, and ash/protein ratio in the bones. Only small differences were observed in the level of amino acids although species specific differences were observed. The levels of Ca and P in lipid free fish bones were about the same in all species analyzed. Fatty acid profile differed in relation to total lipid levels in the fish bones, but some minor differences between fish species were observed.

  3. Usefulness of Fatty Acid Composition for Differentiation of Legionella Species

    PubMed Central

    Diogo, Alexandra; Veríssimo, António; Nobre, M. Fernanda; da Costa, Milton S.

    1999-01-01

    Numerical analysis of fatty acid methyl ester (FAME) profiles of 199 isolates and 76 reference strains, belonging to all validly described species of the genus Legionella that can be cultured in laboratory media, was used to differentiate between the species of this genus. With the exception of the strains that autofluoresced red, it was possible to differentiate all the other Legionella species. The strains of the species L. bozemanii, L. dumoffii, L. feeleii, L. gormanii, L. maceachernii, L. micdadei, and L. quinlivanii did not form single clusters, showing some degree of variability in the fatty acid compositions. The strains of the blue-white autofluorescent species had very similar fatty acid compositions and were difficult to distinguish from each other. Nine isolates had fatty acid profiles unlike those of any of the validly described species and may represent different FAME groups of known species or undescribed Legionella species. The method used in this study was useful for screening and discriminating large number of isolates of Legionella species. Moreover, the results obtained can be included in a database of fatty acid profiles, leading to a more accurate automatic identification of Legionella isolates. PMID:10364593

  4. DNA Barcoding of Shark Meats Identify Species Composition and CITES-Listed Species from the Markets in Taiwan

    PubMed Central

    Liu, Shang-Yin Vanson; Chan, Chia-Ling Carynn; Lin, Oceana; Hu, Chieh-Shen; Chen, Chaolun Allen

    2013-01-01

    Background An increasing awareness of the vulnerability of sharks to exploitation by shark finning has contributed to a growing concern about an unsustainable shark fishery. Taiwan’s fleet has the 4th largest shark catch in the world, accounting for almost 6% of the global figures. Revealing the diversity of sharks consumed by Taiwanese is important in designing conservation plans. However, fins make up less than 5% of the total body weight of a shark, and their bodies are sold as filets in the market, making it difficult or impossible to identify species using morphological traits. Methods In the present study, we adopted a DNA barcoding technique using a 391-bp fragment of the mitochondrial cytochrome oxidase I (COI) gene to examine the diversity of shark filets and fins collected from markets and restaurants island-wide in Taiwan. Results Amongst the 548 tissue samples collected and sequenced, 20 major clusters were apparent by phylogenetic analyses, each of them containing individuals belonging to the same species (most with more than 95% bootstrap values), corresponding to 20 species of sharks. Additionally, Alopias pelagicus, Carcharhinus falciformis, Isurus oxyrinchus, and Prionace glauca consisted of 80% of the samples we collected, indicating that these species might be heavily consumed in Taiwan. Approximately 5% of the tissue samples used in this study were identified as species listed in CITES Appendix II, including two species of Sphyrna, C. longimanus and Carcharodon carcharias. Conclusion DNA barcoding provides an alternative method for understanding shark species composition when species-specific data is unavailable. Considering the global population decline, stock assessments of Appendix II species and highly consumed species are needed to accomplish the ultimate goal of shark conservation. PMID:24260209

  5. Changes in Species Richness and Composition of Tiger Moths (Lepidoptera: Erebidae: Arctiinae) among Three Neotropical Ecoregions.

    PubMed

    Beccacece, Hernán Mario; Zeballos, Sebastián Rodolfo; Zapata, Adriana Inés

    Paraná, Yungas and Chaco Serrano ecoregions are among the most species-rich terrestrial habitats at higher latitude. However, the information for tiger moths, one of the most speciose groups of moths, is unknown in these ecoregions. In this study, we assess their species richness and composition in all three of these ecoregions. Also we investigated whether the species composition of tiger moths is influenced by climatic factors and altitude. Tiger moth species were obtained with samples from 71 sites using standardized protocols (21 sites were in Yungas, 19 in Paraná and 31 in Chaco Serrano). Rarefaction-extrapolation curves, non-parametric estimators for incidence and sample coverage indices were performed to assess species richness in the ecoregions studied. Non metric multidimensional scaling and adonis tests were performed to compare the species composition of tiger moths among ecoregions. Permutest analysis and Pearson correlation were used to evaluate the relationship among species composition and annual mean temperature, annual temperature range, annual precipitation, precipitation seasonality and altitude. Among ecoregions Paraná was the richest with 125 species, followed by Yungas with 63 species and Chaco Serrano with 24 species. Species composition differed among these ecoregions, although Yungas and Chaco Serrano were more similar than Paraná. Species composition was significantly influenced by climatic factors and altitude. This study showed that species richness and species composition of tiger moths differed among the three ecoregions assessed. Furthermore, not only climatic factors and altitude influence the species composition of tiger moths among ecoregions, but also climatic seasonality at higher latitude in Neotropical South America becomes an important factor.

  6. Chemical composition and antioxidant activities of essential oils and methanol extracts of three wild Lavandula L. species.

    PubMed

    Messaoud, C; Chograni, H; Boussaid, M

    2012-11-01

    A comparative study of essential oil composition, polyphenol content and antioxidant activities of Lavandula coronopifolia, Lavandula multifida and Lavandula stoechas subsp. stoechas were reported. Qualitative and quantitative variations in the composition of oils according to species were shown. Lavandula coronopifolia's oil was characterised by high proportions of trans-β-ocimene (26.9%), carvacrol (18.5%), β-bisabolene (13.1%) and myrcene (7.5%). The main components of L. multifida oil are carvacrol (65.1%) and β-bisabolene (24.7%). Lavandula stoechas oil is rich in fenchone (34.3%) and comphor (27.4%). The total phenolic and flavonoid contents also significantly varied among species. Lavandula coronopifolia exhibits the highest phenolic and flavonoid contents (31.3 mg GAE g(-1) and 16.3 mg RE g(-1), respectively), followed by L. multifida (30.8 mg GAE g(-1) and 12.3 mg RE g(-1)). Methanolic extracts and essential oils displayed significant antioxidant activities. The level of antioxidant capacity varied according to extracts and species.

  7. Total mercury levels in commercial fish species from Italian fishery and aquaculture.

    PubMed

    Di Lena, Gabriella; Casini, Irene; Caproni, Roberto; Fusari, Andrea; Orban, Elena

    2017-06-01

    Total mercury levels were measured in 42 commercial fish species caught off the Central Adriatic and Tyrrhenian coasts of Italy and in 6 aquaculture species. The study on wild fish covered species differing in living habitat and trophic level. The study on farmed fish covered marine and freshwater species from intensive and extensive aquaculture and their feed. Mercury levels were analysed by thermal decomposition-amalgamation-atomic absorption spectrophotometry. Total mercury concentrations in the muscle of wild fish showed a high variability among species (0.025-2.20 mg kg -1 wet weight). The lowest levels were detected in low trophic-level demersal and pelagic-neritic fish and in young individuals of high trophic-level species. Levels exceeding the European Commission limits were found in large-size specimens of high trophic-level pelagic and demersal species. Fish from intensive farming showed low levels of total mercury (0.008-0.251 mg kg -1 ). Fish from extensive rearing showed variable contamination levels, depending on the area of provenience. An estimation of the human intake of mercury associated to the consumption of the studied fish and its comparison with the tolerable weekly intake is provided.

  8. [Distribution and species composition of hyporheic macroinvertebrates in a mountain stream].

    PubMed

    Zhang, Yue-wei; Yuan, Xing-zhong; Liu, Hong; Ren, Hai-qing; Deng, Wei; Wang, Xiao-feng

    2015-09-01

    Hyporheic macroinvertebrates are an important component of stream ecosystem. The composition and distribution of the hyporheic macroinvertebrates were investigated using artificial substrates in the upper reaches of Heishuitan River in August, December 2013 and April 2014. The results indicated that a total of 27 microinvertbrate species were identified in all three seasons. In summer, 22 species were identified, accounting for 81.8% of aquatic insects. 16 species were identified both in winter and spring, accounting for 75.0% and 62.5% of aquatic insects, respectively. The density of macroinvertebrate assemblage was significantly lower in summer than in winter and spring, and was the highest in spring. The biomass of macroinvertebrate assemblage was significantly higher in winter than in summer and spring, and was the lowest in summer. Species richness, Shannon index and Pielou index all had no significant difference among the three seasons. The density and richness of macroinvertebrates decreased with bed depth, and the maximum invertebrate density was found within the top 20 cm of the stream bed. Collector-filterer and collector-gatherer were the dominant functional feeding group in all three seasons. The community structure and temporal-spatial distribution of macroinvertebrates were determined by interactions and life history strategy of macroinvertebrates, and physical-chemical factors of hyporheic zone.

  9. Remnant trees affect species composition but not structure of tropical second-growth forest.

    PubMed

    Sandor, Manette E; Chazdon, Robin L

    2014-01-01

    Remnant trees, spared from cutting when tropical forests are cleared for agriculture or grazing, act as nuclei of forest regeneration following field abandonment. Previous studies on remnant trees were primarily conducted in active pasture or old fields abandoned in the previous 2-3 years, and focused on structure and species richness of regenerating forest, but not species composition. Our study is among the first to investigate the effects of remnant trees on neighborhood forest structure, biodiversity, and species composition 20 years post-abandonment. We compared the woody vegetation around individual remnant trees to nearby plots without remnant trees in the same second-growth forests ("control plots"). Forest structure beneath remnant trees did not differ significantly from control plots. Species richness and species diversity were significantly higher around remnant trees. The species composition around remnant trees differed significantly from control plots and more closely resembled the species composition of nearby old-growth forest. The proportion of old-growth specialists and generalists around remnant trees was significantly greater than in control plots. Although previous studies show that remnant trees may initially accelerate secondary forest growth, we found no evidence that they locally affect stem density, basal area, and seedling density at later stages of regrowth. Remnant trees do, however, have a clear effect on the species diversity, composition, and ecological groups of the surrounding woody vegetation, even after 20 years of forest regeneration. To accelerate the return of diversity and old-growth forest species into regrowing forest on abandoned land, landowners should be encouraged to retain remnant trees in agricultural or pastoral fields.

  10. Changes in Species Richness and Composition of Tiger Moths (Lepidoptera: Erebidae: Arctiinae) among Three Neotropical Ecoregions

    PubMed Central

    Beccacece, Hernán Mario; Zeballos, Sebastián Rodolfo; Zapata, Adriana Inés

    2016-01-01

    Paraná, Yungas and Chaco Serrano ecoregions are among the most species-rich terrestrial habitats at higher latitude. However, the information for tiger moths, one of the most speciose groups of moths, is unknown in these ecoregions. In this study, we assess their species richness and composition in all three of these ecoregions. Also we investigated whether the species composition of tiger moths is influenced by climatic factors and altitude. Tiger moth species were obtained with samples from 71 sites using standardized protocols (21 sites were in Yungas, 19 in Paraná and 31 in Chaco Serrano). Rarefaction-extrapolation curves, non-parametric estimators for incidence and sample coverage indices were performed to assess species richness in the ecoregions studied. Non metric multidimensional scaling and adonis tests were performed to compare the species composition of tiger moths among ecoregions. Permutest analysis and Pearson correlation were used to evaluate the relationship among species composition and annual mean temperature, annual temperature range, annual precipitation, precipitation seasonality and altitude. Among ecoregions Paraná was the richest with 125 species, followed by Yungas with 63 species and Chaco Serrano with 24 species. Species composition differed among these ecoregions, although Yungas and Chaco Serrano were more similar than Paraná. Species composition was significantly influenced by climatic factors and altitude. This study showed that species richness and species composition of tiger moths differed among the three ecoregions assessed. Furthermore, not only climatic factors and altitude influence the species composition of tiger moths among ecoregions, but also climatic seasonality at higher latitude in Neotropical South America becomes an important factor. PMID:27681478

  11. Herbivore species identity and composition affect soil enzymatic activity through altered plant composition in a coastal tallgrass prairie

    USDA-ARS?s Scientific Manuscript database

    Although single species of herbivores are known to affect soil microbial communities, the effects of herbivore species identity and functional composition on soil microbes is unknown. We tested the effects of single species of orthopterans and multiple species combinations on soil enzymatic activity...

  12. Methodology for estimation of total body composition in laboratory mammals

    NASA Technical Reports Server (NTRS)

    Pace, N.; Rahlmann, D. F.; Smith, A. H.

    1979-01-01

    A standardized dissection and chemical analysis procedure was developed for individual animals of several species in the size range mouse to monkey (15 g to 15 kg). The standardized procedure permits rigorous comparisons to be made both interspecifically and intraspecifically of organ weights and gross chemical composition in mammalian species series, and was applied successfully to laboratory mice, hamsters, rats, guinea pigs, and rabbits, as well as to macaque monkeys. The procedure is described in detail.

  13. Estimating forest species composition using a multi-sensor approach

    Treesearch

    P.T. Wolter

    2009-01-01

    The magnitude, duration, and frequency of forest disturbance caused by the spruce budworm and forest tent caterpillar has increased over the last century due to a shift in forest species composition linked to historical fire suppression, forest management, and pesticide application that has fostered the increase in dominance of host tree species. Modeling approaches...

  14. Remnant Trees Affect Species Composition but Not Structure of Tropical Second-Growth Forest

    PubMed Central

    Sandor, Manette E.; Chazdon, Robin L.

    2014-01-01

    Remnant trees, spared from cutting when tropical forests are cleared for agriculture or grazing, act as nuclei of forest regeneration following field abandonment. Previous studies on remnant trees were primarily conducted in active pasture or old fields abandoned in the previous 2–3 years, and focused on structure and species richness of regenerating forest, but not species composition. Our study is among the first to investigate the effects of remnant trees on neighborhood forest structure, biodiversity, and species composition 20 years post-abandonment. We compared the woody vegetation around individual remnant trees to nearby plots without remnant trees in the same second-growth forests (“control plots”). Forest structure beneath remnant trees did not differ significantly from control plots. Species richness and species diversity were significantly higher around remnant trees. The species composition around remnant trees differed significantly from control plots and more closely resembled the species composition of nearby old-growth forest. The proportion of old-growth specialists and generalists around remnant trees was significantly greater than in control plots. Although previous studies show that remnant trees may initially accelerate secondary forest growth, we found no evidence that they locally affect stem density, basal area, and seedling density at later stages of regrowth. Remnant trees do, however, have a clear effect on the species diversity, composition, and ecological groups of the surrounding woody vegetation, even after 20 years of forest regeneration. To accelerate the return of diversity and old-growth forest species into regrowing forest on abandoned land, landowners should be encouraged to retain remnant trees in agricultural or pastoral fields. PMID:24454700

  15. Bryophyte Species Richness and Composition along an Altitudinal Gradient in Gongga Mountain, China

    PubMed Central

    Sun, Shou-Qin; Wu, Yan-Hong; Wang, Gen-Xu; Zhou, Jun; Yu, Dong; Bing, Hai-Jian; Luo, Ji

    2013-01-01

    An investigation of terrestrial bryophyte species diversity and community structure along an altitudinal gradient from 2,001 to 4,221 m a.s.l. in Gongga Mountain in Sichuan, China was carried out in June 2010. Factors which might affect bryophyte species composition and diversity, including climate, elevation, slope, depth of litter, vegetation type, soil pH and soil Eh, were examined to understand the altitudinal feature of bryophyte distribution. A total of 14 representative elevations were chosen along an altitudinal gradient, with study sites at each elevation chosen according to habitat type (forests, grasslands) and accessibility. At each elevation, three 100 m × 2 m transects that are 50 m apart were set along the contour line, and three 50 cm × 50 cm quadrats were set along each transect at an interval of 30 m. Species diversity, cover, biomass, and thickness of terrestrial bryophytes were examined. A total of 165 species, including 42 liverworts and 123 mosses, are recorded in Gongga mountain. Ground bryophyte species richness does not show any clear elevation trend. The terrestrial bryophyte cover increases with elevation. The terrestrial bryophyte biomass and thickness display a clear humped relationship with the elevation, with the maximum around 3,758 m. At this altitude, biomass is 700.3 g m−2 and the maximum thickness is 8 cm. Bryophyte distribution is primarily associated with the depth of litter, the air temperature and the precipitation. Further studies are necessary to include other epiphytes types and vascular vegetation in a larger altitudinal range. PMID:23472146

  16. Grasshopper species composition shifts following a severe rangeland grasshopper outbreak

    USDA-ARS?s Scientific Manuscript database

    Little is known about how grasshopper species abundances shift during and following severe outbreaks, as sampling efforts usually end when outbreaks subside. Grasshopper densities, species composition and vegetation have infrequently been sampled during and after a severe outbreak in the western U.S...

  17. Species loss on spatial patterns and composition of zoonotic parasites

    PubMed Central

    Harris, Nyeema C.; Dunn, Robert R.

    2013-01-01

    Species loss can result in the subsequent loss of affiliate species. Though largely ignored to date, these coextinctions can pose threats to human health by altering the composition, quantity and distribution of zoonotic parasites. We simulated host extinctions from more than 1300 host–parasite associations for 29 North American carnivores to investigate changes in parasite composition and species richness. We also explored the geography of zoonotic parasite richness under three carnivore composition scenarios and examined corresponding levels of human exposure. We found that changes in parasite assemblages differed among parasite groups. Because viruses tend to be generalists, the proportion of parasites that are viruses increased as more carnivores went extinct. Coextinction of carnivore parasites is unlikely to be common, given that few specialist parasites exploit hosts of conservation concern. However, local extirpations of widespread carnivore hosts can reduce overall zoonotic richness and shift distributions of parasite-rich areas. How biodiversity influences disease risks remains the subject of debate. Our results make clear that hosts vary in their contribution to human health risks. As a consequence, so too does the loss (or gain) of particular hosts. Anticipating changes in host composition in future environments may help inform parasite conservation and disease mitigation efforts. PMID:24068356

  18. Endurance exercise training and high-fat diet differentially affect composition of diacylglycerol molecular species in rat skeletal muscle.

    PubMed

    Kawanishi, Noriaki; Takagi, Kana; Lee, Hyeon-Cheol; Nakano, Daiki; Okuno, Toshiaki; Yokomizo, Takehiko; Machida, Shuichi

    2018-06-01

    Insulin resistance of peripheral muscle is implicated in the etiology of metabolic syndrome in obesity. Although accumulation of glycerolipids, such as triacylglycerol and diacylglycerol (DAG), in muscle contributes to insulin resistance in obese individuals, endurance-trained athletes also have higher glycerolipid levels but normal insulin sensitivity. We hypothesized that the difference in insulin sensitivity of skeletal muscle between athletes and obese individuals stems from changes in fatty acid composition of accumulated lipids. Here, we evaluated the effects of intense endurance exercise and high-fat diet (HFD) on the accumulation and composition of lipid molecular species in rat skeletal muscle using a lipidomic approach. Sprague-Dawley female rats were randomly assigned to three groups and received either normal diet (ND) in sedentary conditions, ND plus endurance exercise training, or HFD in sedentary conditions. Rats were fed ND or HFD between 4 and 12 wk of age. Rats in the exercise group ran on a treadmill for 120 min/day, 5 days/wk, for 8 wk. Soleus muscle lipidomic profiles were obtained using liquid chromatography/tandem mass spectrometry. Total DAG levels, particularly those of palmitoleate-containing species, were increased in muscle by exercise training. However, whereas the total DAG level in the muscle was also increased by HFD, the levels of DAG molecular species containing palmitoleate were decreased by HFD. The concentration of phosphatidylethanolamine molecular species containing palmitoleate was increased by exercise but decreased by HFD. Our results indicate that although DAG accumulation was similar levels in trained and sedentary obese rats, specific changes in molecular species containing palmitoleate were opposite.

  19. Compositional shifts in Costa Rican forests due to climate-driven species migrations.

    PubMed

    Feeley, Kenneth J; Hurtado, Johanna; Saatchi, Sassan; Silman, Miles R; Clark, David B

    2013-11-01

    Species are predicted to shift their distributions upslope or poleward in response to global warming. This prediction is supported by a growing number of studies documenting species migrations in temperate systems but remains poorly tested for tropical species, and especially for tropical plant species. We analyzed changes in tree species composition in a network of 10 annually censused 1-ha plots spanning an altitudinal gradient of 70-2800 m elevation in Costa Rica. Specifically, we combined plot data with herbarium records (accessed through GBIF) to test if the plots' community temperature scores (CTS, average thermal mean of constituent species weighted by basal area) have increased over the past decade as is predicted by climate-driven species migrations. In addition, we quantified the contributions of stem growth, recruitment, and mortality to the observed patterns. Supporting our a priori hypothesis of upward species migrations, we found that there have been consistent directional shifts in the composition of the plots, such that the relative abundance of lowland species, and hence CTS, increased in 90% of plots. The rate of the observed compositional shifts corresponds to a mean thermal migration rate (TMR) of 0.0065 °C yr(-1) (95% CI = 0.0005-0.0132 °C yr(-1) ). While the overall TMR is slower than predicted based on concurrent regional warming of 0.0167 °C yr(-1) , migrations were on pace with warming in 4 of the 10 plots. The observed shifts in composition were driven primarily by mortality events (i.e., the disproportionate death of highland vs. lowland species), suggesting that individuals of many tropical tree species will not be able to tolerate future warming and thus their persistence in the face of climate change will depend on successful migrations. Unfortunately, in Costa Rica and elsewhere, land area inevitably decreases at higher elevations; hence, even species that are able to migrate successfully will face heightened risks of

  20. Chemical composition of various Ephedra species

    PubMed Central

    Ibragic, Saida; Sofić, Emin

    2015-01-01

    The medicinal significance of Ephedra is based on the sympathomimetic properties of ephedrine (E) alkaloids. Pharmacological effects depend on the phytocomposition of individual Ephedra species. The aim of this study was to measure the total alkaloids content (TAC), total phenolics content (TPC), and total flavonoids content (TFC) and determine their relationship in dry herb of Ephedra major, Ephedra distachya subsp. helvetica, Ephedra monosperma, Ephedra fragilis, Ephedra foeminea, Ephedra alata, Ephedra altissima and Ephedra foliata. Nowadays, medicinal use of Ephedrae herba is limited, but the abuse of its psychostimulants is rising. In this study, TAC, TPC and TFC were determined using spectrophotometric methods. For the first time, ultra-performance liquid chromatography with ultraviolet detection (UPLC-UV) was used for separation and quantification of E-type alkaloids of various Ephedra species. The highest TPC and TFC were found in E. alata (53.3 ± 0.1 mg Gallic acid equivalents/g dry weight, 2.8 mg quercetin equivalents/g dry weight, respectively). The total content of E and pseudoephedrine determined by UPLC-UV varied between 20.8 mg/g dry weight (E. distachya subsp. helvetica) and 34.7 mg/g dry weight (E. monosperma). The variable content and ratio between secondary metabolites determined in different Ephedra species reflects their metabolic activities. Utilization of UPLC-UV unveiled that this technique is sensitive, selective, and useful for separation and quantification of different alkaloids in complex biological matrixes. The limit of detection was 5 ng. Application of UPLC-UV can be recommended in quick analyses of E-type alkaloids in forensic medicine and quality control of pharmaceutical preparations. PMID:26295290

  1. [Seasonal changes of fish species composition and diversity in mudflat wetlands of Hangzhou Bay].

    PubMed

    Jia, Xing-huan; Zhang, Heng; Jiang, Ke-yi; Wu, Ming

    2010-12-01

    In order to understand the spatiotemporal variation of fish species composition and biodiversity in the mudflat wetlands of Hangzhou Bay, thirty six surveys were conducted in the mudflat area, inning area, and aquaculture area in the south bank of the Bay in. March (early spring), May (spring), July (summer), and October (autumn), 2009. A total of 41 species belonging to 9 orders and 16 families were observed, among which, Cyprinid had the largest species number (14 species, 33.3% of the total), followed by Gobiidae (8 species, 19.1%). According to the lifestyle of fish, these 41 species could be divided into five ecological types, i.e., freshwater type (21 species), brackish-water type (16 species), inshore type (2 species), anadromous type (Coilia ectenes), and catadromios type (Anguilla japonica). The fish abundance was the highest (54. 5 fish per net) in summer, followed by in spring and autumn, and the lowest (17.7 fish per net) in early spring. In the three habitats, mudflat area and inning area had the similar seasonal change of fish abundance, i.e., the lowest in early spring, the highest in summer, and then decreased in autumn. Only two or three species were the dominant species in different seasons. In mudflat area, the dominant species were Mugil cephalus and Liza carinatus; while in inning and aquaculture areas, the dominant species were Carassius auratus, Hemiculter leucisculus, and Pseudorasbora parva. The values of Margalef's richness index (D), Pielou's evenness index (J), and Shannon index (H) were lower in March than in other months, but had no significant differences among May, July, and October (P > 0.05). The H value ranged in 0. 27-2. 13, being the lowest in March and higher in May and October (1.66 and 1.63, respectively). Overall, the fish abundance and biodiversity in the mudflat wetlands of Hangzhou Bay had apparent seasonal changes.

  2. Near infra-red spectroscopy quantitative modelling of bivalve protein, lipid and glycogen composition using single-species versus multi-species calibration and validation sets

    NASA Astrophysics Data System (ADS)

    Bartlett, Jill K.; Maher, William A.; Purss, Matthew B. J.

    2018-03-01

    Near infrared spectroscopy (NIRS) quantitative modelling was used to measure the protein, lipid and glycogen composition of five marine bivalve species (Saccostrea glomerata, Ostrea angasi, Crassostrea gigas, Mytilus galloprovincialis and Anadara trapezia) from multiple locations and seasons. Predictive models were produced for each component using individual species and aggregated sample populations for the three oyster species (S. glomerata, O. angasi and C. gigas) and for all five bivalve species. Whole animal tissues were freeze dried, ground to > 20 μm and scanned by NIRS. Protein, lipid and glycogen composition were determined by traditional chemical analyses and calibration models developed to allow rapid NIRS-measurement of these components in the five bivalve species. Calibration modelling was performed using wavelet selection, genetic algorithms and partial least squares analysis. Model quality was assessed using RPIQ and RMESP. For protein composition, single species model results had RPIQ values between 2.4 and 3.5 and RMSEP between 8.6 and 18%, the three oyster model had an RPIQ of 2.6 and an RMSEP of 10.8% and the five bivalve species had an RPIQ of 3.6 and RMSEP of 8.7% respectively. For lipid composition, single species models achieved RPIQ values between 2.9 and 5.3 with RMSEP between 9.1 and 11.2%, the oyster model had an RPIQ of 3.6 and RMSEP of 6.8 and the five bivalve model had an RPIQ of 5.2 and RMSEP of 6.8% respectively. For glycogen composition, the single species models had RPIQs between 3.8 and 18.9 with RMSEP between 3.5 and 9.2%, the oyster model had an RPIQ of 5.5 and RMSEP of 7.1% and the five bivalve model had an RPIQ of 4 and RMSEP of 7.6% respectively. Comparison between individual species models and aggregated models for three oyster species and five bivalve species for each component indicate that aggregating data from like species produces high quality models with robust and reliable quantitative application. The benefit of

  3. Chemical Composition of Selected Commercial Herbal Remedies in Relation to Geographical Origin and Inter-Species Diversity.

    PubMed

    Konieczynski, Pawel; Viapiana, Agnieszka; Lysiuk, Roman; Wesolowski, Marek

    2018-03-01

    Infusions prepared from medicinal herbs that are rich in flavonoids are very popular herbal remedies in societies of Eastern Europe. Therefore, the content of essential elements together with total flavonoids was analyzed in 65 commercially available samples of herbal drugs originating from Ukraine, Romania, and Belarus. The results showed that metallic elements (in mg kg -1 d.w.) have occurred in the following order: Fe > Mn > Zn > Cu, both for total and water-extractable species. Total flavonoids were determined in the range from 10.0 to 191.8 mg g -1 d.w. Several significant correlations have been found between the analytes, especially among water-extractable Fe with other metals, and total flavonoids and Fe, Zn, and Mn. Analysis of variance has revealed significant differences among studied samples due to their origin from different countries, especially between Belarussian samples and others. Differences owing to belonging to various plant species were also found, as it was noticed in the case of Polygoni aviculare herba in comparison with other botanical plant species. Moreover, multivariate statistical techniques, such as cluster analysis (CA) and principal component analysis (PCA) were used to gather herbal drugs based on similarity of chemical composition. CA grouped the samples into clusters with similar level of elements and total flavonoid contents, and PCA has indicated Hyperici herba, Tiliae flores, and Crataegi fructus as herbal remedies with close concentration of studied elements and flavonoids.

  4. Structure and species composition of novel forests dominated by an introduced species in northcentral Puerto Rico

    Treesearch

    Oscar J. Abelleira Martinez; Mariela A. Rodríguez; Ivonne Rosario; Nataly Soto; Armando López; Ariel E. Lugo

    2010-01-01

    The African tulip tree, Spathodea campanulata Beauv., is an introduced species forming novel forest types in Puerto Rico. These forests develop naturally after deforestation, agricultural use and land abandonment, and there are many questions as to their ecological characteristics. We sampled structure and species composition of large, small, and juvenile trees (C10,...

  5. Changes in plant biomass and species composition of alpine Kobresia meadows along altitudinal gradient on the Qinghai-Tibetan Plateau.

    PubMed

    Wang, ChangTing; Cao, GuangMin; Wang, QiLan; Jing, ZengChun; Ding, LuMing; Long, RuiJun

    2008-01-01

    Alpine Kobresia meadows are major vegetation types on the Qinghai-Tibetan Plateau. There is growing concern over their relationships among biodiversity, productivity and environments. Despite the importance of species composition, species richness, the type of different growth forms, and plant biomass structure for Kobresia meadow ecosystems, few studies have been focused on the relationship between biomass and environmental gradient in the Kobresia meadow plant communities, particularly in relation to soil moisture and edaphic gradients. We measured the plant species composition, herbaceous litter, aboveground and belowground biomass in three Kobresia meadow plant communities in Haibei Alpine Meadow Ecosystem Research Station from 2001 to 2004. Community differences in plant species composition were reflected in biomass distribution. The total biomass showed a decrease from 13196.96+/-719.69 g/m(2) in the sedge-dominated K. tibetica swamp to 2869.58+/-147.52 g/m(2) in the forb and sedge dominated K. pygmaea meadow, and to 2153.08+/-141.95 g/m(2) in the forbs and grasses dominated K. humilis along with the increase of altitude. The vertical distribution of belowground biomass is distinct in the three meadow communities, and the belowground biomass at the depth of 0-10 cm in K. tibetica swamp meadow was significantly higher than that in K. humilis and K. pygmaea meadows (P<0.01). The herbaceous litter in K. tibetica swamp was significantly higher than those in K. pygnaeca and K. humilis meadows. The effects of plant litter are enhanced when ground water and soil moisture levels are raised. The relative importance of litter and vegetation may vary with soil water availability. In the K. tibetica swamp, total biomass was negatively correlated to species richness (P<0.05); aboveground biomass was positively correlated to soil organic matter, soil moisture, and plant cover (P<0.05); belowground biomass was positively correlated with soil moisture (P<0.05). However, in

  6. Consideration of species community composition in statistical ...

    EPA Pesticide Factsheets

    Diseases are increasing in marine ecosystems, and these increases have been attributed to a number of environmental factors including climate change, pollution, and overfishing. However, many studies pool disease prevalence into taxonomic groups, disregarding host species composition when comparing sites or assessing environmental impacts on patterns of disease presence. We used simulated data under a known environmental effect to assess the ability of standard statistical methods (binomial and linear regression, ANOVA) to detect a significant environmental effect on pooled disease prevalence with varying species abundance distributions and relative susceptibilities to disease. When one species was more susceptible to a disease and both species only partially overlapped in their distributions, models tended to produce a greater number of false positives (Type I error). Differences in disease risk between regions or along an environmental gradient tended to be underestimated, or even in the wrong direction, when highly susceptible taxa had reduced abundances in impacted sites, a situation likely to be common in nature. Including relative abundance as an additional variable in regressions improved model accuracy, but tended to be conservative, producing more false negatives (Type II error) when species abundance was strongly correlated with the environmental effect. Investigators should be cautious of underlying assumptions of species similarity in susceptib

  7. Proximate composition and mineral content of two edible species of Cnidoscolus (tree spinach).

    PubMed

    Kuti, J O; Kuti, H O

    1999-01-01

    Proximate composition and mineral content of raw and cooked leaves of two edible tree spinach species (Cnidoscolus chayamansa and C. aconitifolius), known locally as 'chaya', were determined and compared with that of a traditional green vegetable, spinach (Spinicia oleraceae). Results of the study indicated that the edible leafy parts of the two chaya species contained significantly (p<0.05) greater amounts of crude protein, crude fiber, Ca, K, Fe, ascorbic acid and beta-carotene than the spinach leaf. However, no significant (p>0.05) differences were found in nutritional composition and mineral content between the chaya species, except minor differences in the relative composition of fatty acids, protein and amino acids. Cooking of chaya leaves slightly reduced nutritional composition of both chaya species. Cooking is essential prior to consumption to inactivate the toxic hydrocyanic glycosides present in chaya leaves. Based on the results of this study, the edible chaya leaves may be good dietary sources of minerals (Ca, K and Fe) and vitamins (ascorbic acid and beta-carotene).

  8. Spatial variability in plant species composition and peatland carbon exchange

    NASA Astrophysics Data System (ADS)

    Goud, E.; Moore, T. R.; Roulet, N. T.

    2015-12-01

    Plant species shifts in response to global change will have significant impacts on ecosystem carbon (C) exchange and storage arising from changes in hydrology. Spatial variation in peatland C fluxes have largely been attributed to the spatial distribution of microhabitats that arise from variation in surface topography and water table depth, but little is known about how plant species composition impacts peatland C cycling or how these impacts will be influenced by changing environmental conditions. We quantified the effect of species composition and environmental variables on carbon dioxide (CO2) and methane (CH4) fluxes over 2 years in a temperate peatland for four plant communities situated along a water table gradient from ombrotrophic bog to beaver pond. We hypothesized that (i) spatial heterogeneity in species composition would drive predictable spatial heterogeneity in C fluxes due to variation in plant traits and ecological tolerances, and (ii) increases in peat temperature would increase C fluxes. Species had different effects on C fluxes primarily due to differences in leaf traits. Differences in ecological tolerances among communities resulted in different rates of CO2 exchange in response to changes in water table depth. There was an overall reduction in ecosystem respiration (ER), gross primary productivity (GPP) and CH4 flux in response to colder peat temperatures in the second year, and the additive effects of a deeper water table in the bog margin and pond sites further reduced flux rates in these areas. These results demonstrate that different plant species can increase or decrease the flux of C into and out of peatlands based on differences in leaf traits and ecological tolerances, and that CO2 and CH4 fluxes are sensitive to changes in soil temperature, especially when coupled with changes in moisture availability.

  9. Nutritional composition and total collagen content of three commercially important edible jellyfish.

    PubMed

    Khong, Nicholas M H; Yusoff, Fatimah Md; Jamilah, B; Basri, Mahiran; Maznah, I; Chan, Kim Wei; Nishikawa, Jun

    2016-04-01

    The study aimed to evaluate nutraceutical potential of three commercially significant edible jellyfish species (Acromitus hardenbergi, Rhopilema hispidum and Rhopilema esculentum). The bell and oral arms of these jellyfishes were analyzed for their proximate composition, calorific value, collagen content, amino acid profile, chemical score and elemental constituent. In general, all jellyfish possessed low calorific values (1.0-4.9 kcal/g D.W.) and negligible fat contents (0.4-1.8 g/100 g D.W.), while protein (20.0-53.9 g/100 g D.W.) and minerals (15.9-57.2g/100g D.W.) were found to be the richest components. Total collagen content of edible jellyfish varied from 122.64 to 693.92 mg/g D.W., accounting for approximately half its total protein content. The dominant amino acids in both bell and oral arms of all jellyfish studied includes glycine, glutamate, threonine, proline, aspartate and arginine, while the major elements were sodium, potassium, chlorine, magnesium, sulfur, zinc and silicon. Among the jellyfish, A. hardenbergi exhibited significantly higher total amino acids, chemical scores and collagen content (p<0.05) compared to R. hispidum and R. esculentum. Having good protein quality and low calories, edible jellyfish is an appealing source of nutritive ingredients for the development of oral formulations, nutricosmetics and functional food. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Near infra-red spectroscopy quantitative modelling of bivalve protein, lipid and glycogen composition using single-species versus multi-species calibration and validation sets.

    PubMed

    Bartlett, Jill K; Maher, William A; Purss, Matthew B J

    2018-03-15

    Near infrared spectroscopy (NIRS) quantitative modelling was used to measure the protein, lipid and glycogen composition of five marine bivalve species (Saccostrea glomerata, Ostrea angasi, Crassostrea gigas, Mytilus galloprovincialis and Anadara trapezia) from multiple locations and seasons. Predictive models were produced for each component using individual species and aggregated sample populations for the three oyster species (S. glomerata, O. angasi and C. gigas) and for all five bivalve species. Whole animal tissues were freeze dried, ground to >20μm and scanned by NIRS. Protein, lipid and glycogen composition were determined by traditional chemical analyses and calibration models developed to allow rapid NIRS-measurement of these components in the five bivalve species. Calibration modelling was performed using wavelet selection, genetic algorithms and partial least squares analysis. Model quality was assessed using RPIQ and RMESP. For protein composition, single species model results had RPIQ values between 2.4 and 3.5 and RMSEP between 8.6 and 18%, the three oyster model had an RPIQ of 2.6 and an RMSEP of 10.8% and the five bivalve species had an RPIQ of 3.6 and RMSEP of 8.7% respectively. For lipid composition, single species models achieved RPIQ values between 2.9 and 5.3 with RMSEP between 9.1 and 11.2%, the oyster model had an RPIQ of 3.6 and RMSEP of 6.8 and the five bivalve model had an RPIQ of 5.2 and RMSEP of 6.8% respectively. For glycogen composition, the single species models had RPIQs between 3.8 and 18.9 with RMSEP between 3.5 and 9.2%, the oyster model had an RPIQ of 5.5 and RMSEP of 7.1% and the five bivalve model had an RPIQ of 4 and RMSEP of 7.6% respectively. Comparison between individual species models and aggregated models for three oyster species and five bivalve species for each component indicate that aggregating data from like species produces high quality models with robust and reliable quantitative application. The benefit of

  11. Landscape composition and habitat area affects butterfly species richness in semi-natural grasslands.

    PubMed

    Ockinger, Erik; Smith, Henrik G

    2006-09-01

    During the last 50 years, the distribution and abundance of many European butterfly species associated with semi-natural grasslands have declined. This may be the result of deteriorating habitat quality, but habitat loss, resulting in decreasing area and increasing isolation of remaining habitat, is also predicted to result in reduced species richness. To investigate the effects of habitat loss on species richness, we surveyed butterflies in semi-natural grasslands of similar quality and structure, but situated in landscapes of different habitat composition. Using spatially explicit habitat data, we selected one large (6-10 ha) and one small (0.5-2 ha) grassland site (pasture) in each of 24 non-overlapping 28.2 km(2) landscapes belonging to three categories differing in the proportion of the area that consisted of semi-natural grasslands. After controlling for local habitat quality, species richness was higher in grassland sites situated in landscapes consisting of a high proportion of grasslands. Species richness was also higher in larger grassland sites, and this effect was more pronounced for sedentary than for mobile species. However, the number of species for a given area did not differ between large and small grasslands. There was also a significant relationship between butterfly species richness and habitat quality in the form of vegetation height and abundance of flowers. In contrast, butterfly density was not related to landscape composition or grassland size. When species respond differently to habitat area or landscape composition this leads to effects on community structure, and nestedness analysis showed that depauperate communities were subsets of richer ones. Both grassland area and landscape composition may have contributed to this pattern, implying that small habitat fragments and landscapes with low proportions of habitat are both likely to mainly contain common generalist species. Based on these results, conservation efforts should aim at

  12. The Ecuadorian Artisanal Fishery for Large Pelagics: Species Composition and Spatio-Temporal Dynamics

    PubMed Central

    Martínez-Ortiz, Jimmy; Aires-da-Silva, Alexandre M.; Lennert-Cody, Cleridy E.; Maunder, Mark N.

    2015-01-01

    The artisanal fisheries of Ecuador operate within one of the most dynamic and productive marine ecosystems of the world. This study investigates the catch composition of the Ecuadorian artisanal fishery for large pelagic fishes, including aspects of its spatio-temporal dynamics. The analyses of this study are based on the most extensive dataset available to date for this fishery: a total of 106,963 trip-landing inspection records collected at its five principal ports during 2008 ‒ 2012. Ecuadorian artisanal fisheries remove a substantial amount of biomass from the upper trophic-level predatory fish community of the eastern tropical Pacific Ocean. It is estimated that at least 135 thousand metric tons (mt) (about 15.5 million fish) were landed in the five principal ports during the study period. The great novelty of Ecuadorian artisanal fisheries is the “oceanic-artisanal” fleet component, which consists of mother-ship (nodriza) boats with their towed fiber-glass skiffs (fibras) operating with pelagic longlines. This fleet has fully expanded into oceanic waters as far offshore as 100°W, west of the Galapagos Archipelago. It is estimated that nodriza operations produce as much as 80% of the total catches of the artisanal fishery. The remainder is produced by independent fibras operating in inshore waters with pelagic longlines and/or surface gillnets. A multivariate regression tree analysis was used to investigate spatio-environmental effects on the nodriza fleet (n = 6,821 trips). The catch species composition of the nodriza fleet is strongly influenced by the northwesterly circulation of the Humboldt Current along the coast of Peru and its associated cold waters masses. The target species and longline gear-type used by nodrizas change seasonally with the incursion of cool waters (< 25°C) from the south and offshore. During this season, dolphinfish (Coryphaena hippurus) dominates the catches. However, in warmer waters, the fishery changes to tuna

  13. The Ecuadorian Artisanal Fishery for Large Pelagics: Species Composition and Spatio-Temporal Dynamics.

    PubMed

    Martínez-Ortiz, Jimmy; Aires-da-Silva, Alexandre M; Lennert-Cody, Cleridy E; Maunder, Mark N

    2015-01-01

    The artisanal fisheries of Ecuador operate within one of the most dynamic and productive marine ecosystems of the world. This study investigates the catch composition of the Ecuadorian artisanal fishery for large pelagic fishes, including aspects of its spatio-temporal dynamics. The analyses of this study are based on the most extensive dataset available to date for this fishery: a total of 106,963 trip-landing inspection records collected at its five principal ports during 2008 ‒ 2012. Ecuadorian artisanal fisheries remove a substantial amount of biomass from the upper trophic-level predatory fish community of the eastern tropical Pacific Ocean. It is estimated that at least 135 thousand metric tons (mt) (about 15.5 million fish) were landed in the five principal ports during the study period. The great novelty of Ecuadorian artisanal fisheries is the "oceanic-artisanal" fleet component, which consists of mother-ship (nodriza) boats with their towed fiber-glass skiffs (fibras) operating with pelagic longlines. This fleet has fully expanded into oceanic waters as far offshore as 100°W, west of the Galapagos Archipelago. It is estimated that nodriza operations produce as much as 80% of the total catches of the artisanal fishery. The remainder is produced by independent fibras operating in inshore waters with pelagic longlines and/or surface gillnets. A multivariate regression tree analysis was used to investigate spatio-environmental effects on the nodriza fleet (n = 6,821 trips). The catch species composition of the nodriza fleet is strongly influenced by the northwesterly circulation of the Humboldt Current along the coast of Peru and its associated cold waters masses. The target species and longline gear-type used by nodrizas change seasonally with the incursion of cool waters (< 25°C) from the south and offshore. During this season, dolphinfish (Coryphaena hippurus) dominates the catches. However, in warmer waters, the fishery changes to tuna

  14. [Effects of highway on the vegetation species composition along a distance gradient from road edge in southeastern margin of Tengeer Desert].

    PubMed

    Feng, Li; Li, Xin-Rong; Guo, Qun; Zhang, Jing-Guang; Zhang, Zhi-Shan

    2011-05-01

    Aimed to examine the effects of highway on the vegetation species composition in arid desert area, forty-eight transects perpendicular to the provincial highway 201 from Shapotou to Jing-tai in the southeastern margin of Tengger Desert were installed, with the vegetation species distribution along a distance gradient from the road edge investigated. The results showed that with increasing distance from the road edge, the species number, coverage, biomass, and alpha-diversity of herbaceous plants declined, but had no significant differences with the control beyond 5 m. Within 0-6 m to the road edge, the herbaceous plant height was greater than that of the control, but their density had less change. Within 0-2 m to the road edge, the species turnover rate of herbaceous plants was lower; at 2-5m, this rate was the highest; while beyond 10 m, the species composition of herbaceous plants was similar to that of the control. The herbaceous plant community at the road edge was dominated by gramineous plants, with the disturbance-tolerant species Pennisetum centrasiaticum, Chloris virgata, and Agropyron cristatum accounting for 68.6% of the total. C. virgata beyond 1 m to the road edge had a rapid decrease in its individual number and presence frequency, P. centrasiaticum and A. cristatum beyond 2 m also showed a similar trend, while the composite plants Artemisia capillaris and A. frigida beyond 2 m from the road edge had a rapid increase in its individual number, accounting for 70% of the herbaceous plants. At the road edge, the coverage and density of shrubs were significantly lower than those of the control, but the species composition had no significant difference.

  15. Shifting plant species composition in response to climate change stabilizes grassland primary production

    PubMed Central

    Liu, Huiying; Mi, Zhaorong; Lin, Li; Wang, Yonghui; Zhang, Zhenhua; Zhang, Fawei; Wang, Hao; Liu, Lingli; Zhu, Biao; Cao, Guangmin; Zhao, Xinquan; Sanders, Nathan J.; Reich, Peter B.

    2018-01-01

    The structure and function of alpine grassland ecosystems, including their extensive soil carbon stocks, are largely shaped by temperature. The Tibetan Plateau in particular has experienced significant warming over the past 50 y, and this warming trend is projected to intensify in the future. Such climate change will likely alter plant species composition and net primary production (NPP). Here we combined 32 y of observations and monitoring with a manipulative experiment of temperature and precipitation to explore the effects of changing climate on plant community structure and ecosystem function. First, long-term climate warming from 1983 to 2014, which occurred without systematic changes in precipitation, led to higher grass abundance and lower sedge abundance, but did not affect aboveground NPP. Second, an experimental warming experiment conducted over 4 y had no effects on any aspect of NPP, whereas drought manipulation (reducing precipitation by 50%), shifted NPP allocation belowground without affecting total NPP. Third, both experimental warming and drought treatments, supported by a meta-analysis at nine sites across the plateau, increased grass abundance at the expense of biomass of sedges and forbs. This shift in functional group composition led to deeper root systems, which may have enabled plant communities to acquire more water and thus stabilize ecosystem primary production even with a changing climate. Overall, our study demonstrates that shifting plant species composition in response to climate change may have stabilized primary production in this high-elevation ecosystem, but it also caused a shift from aboveground to belowground productivity. PMID:29666319

  16. Shifting plant species composition in response to climate change stabilizes grassland primary production.

    PubMed

    Liu, Huiying; Mi, Zhaorong; Lin, Li; Wang, Yonghui; Zhang, Zhenhua; Zhang, Fawei; Wang, Hao; Liu, Lingli; Zhu, Biao; Cao, Guangmin; Zhao, Xinquan; Sanders, Nathan J; Classen, Aimée T; Reich, Peter B; He, Jin-Sheng

    2018-04-17

    The structure and function of alpine grassland ecosystems, including their extensive soil carbon stocks, are largely shaped by temperature. The Tibetan Plateau in particular has experienced significant warming over the past 50 y, and this warming trend is projected to intensify in the future. Such climate change will likely alter plant species composition and net primary production (NPP). Here we combined 32 y of observations and monitoring with a manipulative experiment of temperature and precipitation to explore the effects of changing climate on plant community structure and ecosystem function. First, long-term climate warming from 1983 to 2014, which occurred without systematic changes in precipitation, led to higher grass abundance and lower sedge abundance, but did not affect aboveground NPP. Second, an experimental warming experiment conducted over 4 y had no effects on any aspect of NPP, whereas drought manipulation (reducing precipitation by 50%), shifted NPP allocation belowground without affecting total NPP. Third, both experimental warming and drought treatments, supported by a meta-analysis at nine sites across the plateau, increased grass abundance at the expense of biomass of sedges and forbs. This shift in functional group composition led to deeper root systems, which may have enabled plant communities to acquire more water and thus stabilize ecosystem primary production even with a changing climate. Overall, our study demonstrates that shifting plant species composition in response to climate change may have stabilized primary production in this high-elevation ecosystem, but it also caused a shift from aboveground to belowground productivity.

  17. Tribological characteristics of a composite total-surface hip replacement

    NASA Technical Reports Server (NTRS)

    Jones, W. R., Jr.; Roberts, J. C.; Ling, F. F.

    1982-01-01

    Continuous fiber, woven E glass composite femoral shells having the same elastic properties as bone were fabricated. The shells were then encrusted with filled epoxy wear resistant coatings and run dry against ultrahigh molecular weight polyethylene acetabular cups in 42,000 and 250,000 cycle wear tests on a total hip simulator. The tribological characteristics of these continuous fiber particulate composite femoral shells articulating with ultrahigh molecular weight polyethylene acetabular cups were comparable to those of a vitallium ball articulating with an ultrahigh molecular weight polyethylene acetabular cup.

  18. Dinucleotide Composition in Animal RNA Viruses Is Shaped More by Virus Family than by Host Species

    PubMed Central

    Di Giallonardo, Francesca; Schlub, Timothy E.; Shi, Mang

    2017-01-01

    ABSTRACT Viruses use the cellular machinery of their hosts for replication. It has therefore been proposed that the nucleotide and dinucleotide compositions of viruses should match those of their host species. If this is upheld, it may then be possible to use dinucleotide composition to predict the true host species of viruses sampled in metagenomic surveys. However, it is also clear that different taxonomic groups of viruses tend to have distinctive patterns of dinucleotide composition that may be independent of host species. To determine the relative strength of the effect of host versus virus family in shaping dinucleotide composition, we performed a comparative analysis of 20 RNA virus families from 15 host groupings, spanning two animal phyla and more than 900 virus species. In particular, we determined the odds ratios for the 16 possible dinucleotides and performed a discriminant analysis to evaluate the capability of virus dinucleotide composition to predict the correct virus family or host taxon from which it was isolated. Notably, while 81% of the data analyzed here were predicted to the correct virus family, only 62% of these data were predicted to their correct subphylum/class host and a mere 32% to their correct mammalian order. Similarly, dinucleotide composition has a weak predictive power for different hosts within individual virus families. We therefore conclude that dinucleotide composition is generally uniform within a virus family but less well reflects that of its host species. This has obvious implications for attempts to accurately predict host species from virus genome sequences alone. IMPORTANCE Determining the processes that shape virus genomes is central to understanding virus evolution and emergence. One question of particular importance is why nucleotide and dinucleotide frequencies differ so markedly between viruses. In particular, it is currently unclear whether host species or virus family has the biggest impact on dinucleotide

  19. Dinucleotide Composition in Animal RNA Viruses Is Shaped More by Virus Family than by Host Species.

    PubMed

    Di Giallonardo, Francesca; Schlub, Timothy E; Shi, Mang; Holmes, Edward C

    2017-04-15

    Viruses use the cellular machinery of their hosts for replication. It has therefore been proposed that the nucleotide and dinucleotide compositions of viruses should match those of their host species. If this is upheld, it may then be possible to use dinucleotide composition to predict the true host species of viruses sampled in metagenomic surveys. However, it is also clear that different taxonomic groups of viruses tend to have distinctive patterns of dinucleotide composition that may be independent of host species. To determine the relative strength of the effect of host versus virus family in shaping dinucleotide composition, we performed a comparative analysis of 20 RNA virus families from 15 host groupings, spanning two animal phyla and more than 900 virus species. In particular, we determined the odds ratios for the 16 possible dinucleotides and performed a discriminant analysis to evaluate the capability of virus dinucleotide composition to predict the correct virus family or host taxon from which it was isolated. Notably, while 81% of the data analyzed here were predicted to the correct virus family, only 62% of these data were predicted to their correct subphylum/class host and a mere 32% to their correct mammalian order. Similarly, dinucleotide composition has a weak predictive power for different hosts within individual virus families. We therefore conclude that dinucleotide composition is generally uniform within a virus family but less well reflects that of its host species. This has obvious implications for attempts to accurately predict host species from virus genome sequences alone. IMPORTANCE Determining the processes that shape virus genomes is central to understanding virus evolution and emergence. One question of particular importance is why nucleotide and dinucleotide frequencies differ so markedly between viruses. In particular, it is currently unclear whether host species or virus family has the biggest impact on dinucleotide frequencies and

  20. Comparative fatty acid composition of four Sargassum species (Fucales, Phaeophyta)

    NASA Astrophysics Data System (ADS)

    Wu, Xiang-Chun; Lu, Bao-Ren; Tseng, C. K.

    1995-12-01

    Fatty acid composition of four Sargassum species from Qingdao and Shidao, Shandong Province was investigated. 16:0 (palmitic acid) was the major saturated fatty acid. C18 and C20 were the main polyunsaturated fatty acids (PUFAs). Arachidonic acid and eicosapentaenoic acid predominated among polyenoic acids in all the algal species examined, except for Sargassum sp. which had low concentration of eicosapentaenoic acid.

  1. Species effects on ecosystem processes are modified by faunal responses to habitat composition.

    PubMed

    Bulling, Mark T; Solan, Martin; Dyson, Kirstie E; Hernandez-Milian, Gema; Luque, Patricia; Pierce, Graham J; Raffaelli, Dave; Paterson, David M; White, Piran C L

    2008-12-01

    Heterogeneity is a well-recognized feature of natural environments, and the spatial distribution and movement of individual species is primarily driven by resource requirements. In laboratory experiments designed to explore how different species drive ecosystem processes, such as nutrient release, habitat heterogeneity is often seen as something which must be rigorously controlled for. Most small experimental systems are therefore spatially homogeneous, and the link between environmental heterogeneity and its effects on the redistribution of individuals and species, and on ecosystem processes, has not been fully explored. In this paper, we used a mesocosm system to investigate the relationship between habitat composition, species movement and sediment nutrient release for each of four functionally contrasting species of marine benthic invertebrate macrofauna. For each species, various habitat configurations were generated by selectively enriching patches of sediment with macroalgae, a natural source of spatial variability in intertidal mudflats. We found that the direction and extent of faunal movement between patches differs with species identity, density and habitat composition. Combinations of these factors lead to concomitant changes in nutrient release, such that habitat composition effects are modified by species identity (in the case of NH4-N) and by species density (in the case of PO4-P). It is clear that failure to accommodate natural patterns of spatial heterogeneity in such studies may result in an incomplete understanding of system behaviour. This will be particularly important for future experiments designed to explore the effects of species richness on ecosystem processes, where the complex interactions reported here for single species may be compounded when species are brought together in multi-species combinations.

  2. Species composition and morphology of protostrongylids (Nematoda: Protostrongylidae) in ruminants from Bulgaria.

    PubMed

    Panayotova-Pencheva, Mariana Stancheva

    2011-10-01

    Lungs of 52 ruminants from different regions of Bulgaria, 16 from goats (Capra aegagrus f. domestica L.), 15 from sheep (Ovis ammon f. domestica L.), 11 from mouflons (Ovis musimon L.), and 10 from chamois (Rupicapra rupicapra L.), were investigated. The aim of the study was to determine the species composition of small lungworms in these hosts. The obtained results are summarized with those of previous studies, and a picture of the present status of the species composition of protostrongylids in ruminants from Bulgaria is forwarded. Morphometric data about the species Muellerius capillaris, Cystocaulus ocreatus, Neostrongylus linearis, Protostrongylus brevispiculum, and Protostrongylus rufescens are presented. The data on the morphology of these five species are supplied for the first time both for Bulgaria and the south-east part of the European continent.

  3. BOREAS TGB-3 Plant Species Composition Data over the NSA Fen

    NASA Technical Reports Server (NTRS)

    Bubier, Jill L.; Hall, Forrest G. (Editor); Conrad, Sara K. (Editor)

    2000-01-01

    The BOReal Ecosystem-Atmosphere Study Trace Gas Biogeochemistry (BOREAS TGB-3) team collected several data sets that contributed to understanding the measured trace gas fluxes over sites in the Northern Study Area (NSA). This data set contains information about the composition of plant species that were within the collars used to measure Net Ecosystem Exchange of CO2 (NEE). The species composition was identified to understand the differences in NEE among the various plant communities in the NSA fen. The data were collected in July of 1994 and 1996. The data are contained in comma-delimited, ASCII files.

  4. Species Composition at the Sub-Meter Level in Discontinuous Permafrost in Subarctic Sweden

    NASA Astrophysics Data System (ADS)

    Anderson, S. M.; Palace, M. W.; Layne, M.; Varner, R. K.; Crill, P. M.

    2013-12-01

    Northern latitudes are experiencing rapid warming. Wetlands underlain by permafrost are particularly vulnerable to warming which results in changes in vegetative cover. Specific species have been associated with greenhouse gas emissions therefore knowledge of species compositional shift allows for the systematic change and quantification of emissions and changes in such emissions. Species composition varies on the sub-meter scale based on topography and other microsite environmental parameters. This complexity and the need to scale vegetation to the landscape level proves vital in our estimation of carbon dioxide (CO2) and methane (CH4) emissions and dynamics. Stordalen Mire (68°21'N, 18°49'E) in Abisko and is located at the edge of discontinuous permafrost zone. This provides a unique opportunity to analyze multiple vegetation communities in a close proximity. To do this, we randomly selected 25 1x1 meter plots that were representative of five major cover types: Semi-wet, wet, hummock, tall graminoid, and tall shrub. We used a quadrat with 64 sub plots and measured areal percent cover for 24 species. We collected ground based remote sensing (RS) at each plot to determine species composition using an ADC-lite (near infrared, red, green) and GoPro (red, blue, green). We normalized each image based on a Teflon white chip placed in each image. Textural analysis was conducted on each image for entropy, angular second momentum, and lacunarity. A logistic regression was developed to examine vegetation cover types and remote sensing parameters. We used a multiple linear regression using forwards stepwise variable selection. We found statistical difference in species composition and diversity indices between vegetation cover types. In addition, we were able to build regression model to significantly estimate vegetation cover type as well as percent cover for specific key vegetative species. This ground-based remote sensing allows for quick quantification of vegetation

  5. Species Composition of Down Dead and Standing Live Trees: Implications for Forest Inventory Analysis

    Treesearch

    Christopher W. Woodall; Linda Nagel

    2005-01-01

    The assessment of species composition in most forest inventory analysis relies solely on standing live tree information characterized by current forest type. With the implementation of the third phase of the U.S. Department of Agriculture Forest Service's Forest Inventory and Analysis program, the species composition of down dead trees, otherwise termed coarse...

  6. Wood Chemical Composition in Species of Cactaceae: The Relationship between Lignification and Stem Morphology

    PubMed Central

    Canché-Escamilla, Gonzalo; Soto-Hernández, Marcos

    2015-01-01

    In Cactaceae, wood anatomy is related to stem morphology in terms of the conferred support. In species of cacti with dimorphic wood, a unique process occurs in which the cambium stops producing wide-band tracheids (WBTs) and produces fibers; this is associated with the aging of individuals and increases in size. Stem support and lignification have only been studied in fibrous tree-like species, and studies in species with WBTs or dimorphic wood are lacking. In this study, we approach this process with a chemical focus, emphasizing the role of wood lignification. We hypothesized that the degree of wood lignification in Cactaceae increases with height of the species and that its chemical composition varies with wood anatomy. To test this, we studied the chemical composition (cellulose, hemicellulose, and lignin content) in 13 species (2 WBTs wood, 3 dimorphic, and 8 fibrous) with contrasting growth forms. We also analyzed lignification in dimorphic and fibrous species to determine the chemical features of WBTs and fibers and their relationship with stem support. The lignin contents were characterized by Fourier transform infrared spectroscopy and high performance liquid chromatography. We found that 11 species have a higher percentage (>35%) of lignin in their wood than other angiosperms or gymnosperms. The lignin chemical composition in fibrous species is similar to that of other dicots, but it is markedly heterogeneous in non-fibrous species where WBTs are abundant. The lignification in WBTs is associated with the resistance to high water pressure within cells rather than the contribution to mechanical support. Dimorphic wood species are usually richer in syringyl lignin, and tree-like species with lignified rays have more guaiacyl lignin. The results suggest that wood anatomy and lignin distribution play an important role in the chemical composition of wood, and further research is needed at the cellular level. PMID:25880223

  7. Wood chemical composition in species of Cactaceae: the relationship between lignification and stem morphology.

    PubMed

    Reyes-Rivera, Jorge; Canché-Escamilla, Gonzalo; Soto-Hernández, Marcos; Terrazas, Teresa

    2015-01-01

    In Cactaceae, wood anatomy is related to stem morphology in terms of the conferred support. In species of cacti with dimorphic wood, a unique process occurs in which the cambium stops producing wide-band tracheids (WBTs) and produces fibers; this is associated with the aging of individuals and increases in size. Stem support and lignification have only been studied in fibrous tree-like species, and studies in species with WBTs or dimorphic wood are lacking. In this study, we approach this process with a chemical focus, emphasizing the role of wood lignification. We hypothesized that the degree of wood lignification in Cactaceae increases with height of the species and that its chemical composition varies with wood anatomy. To test this, we studied the chemical composition (cellulose, hemicellulose, and lignin content) in 13 species (2 WBTs wood, 3 dimorphic, and 8 fibrous) with contrasting growth forms. We also analyzed lignification in dimorphic and fibrous species to determine the chemical features of WBTs and fibers and their relationship with stem support. The lignin contents were characterized by Fourier transform infrared spectroscopy and high performance liquid chromatography. We found that 11 species have a higher percentage (>35%) of lignin in their wood than other angiosperms or gymnosperms. The lignin chemical composition in fibrous species is similar to that of other dicots, but it is markedly heterogeneous in non-fibrous species where WBTs are abundant. The lignification in WBTs is associated with the resistance to high water pressure within cells rather than the contribution to mechanical support. Dimorphic wood species are usually richer in syringyl lignin, and tree-like species with lignified rays have more guaiacyl lignin. The results suggest that wood anatomy and lignin distribution play an important role in the chemical composition of wood, and further research is needed at the cellular level.

  8. Species composition and seasonal abundance of sandflies (Diptera: Psychodidae: Phlebotominae) in coffee agroecosystems

    PubMed Central

    Pérez, Jeanneth; Virgen, Armando; Rojas, Julio Cesar; Rebollar-Téllez, Eduardo Alfonso; Alfredo, Castillo; Infante, Francisco; Mikery, Oscar; Marina, Carlos Felix; Ibáñez-Bernal, Sergio

    2013-01-01

    The composition and seasonal occurrence of sandflies were investigated in coffee agroecosystems in the Soconusco region of Chiapas, Mexico. Insect sampling was performed on three plantations located at different altitudes: Finca Guadalupe Zajú [1,000 m above sea level (a.s.l.)], Finca Argovia (613 m a.s.l.) and Teotihuacán del Valle (429 m a.s.l.). Sandflies were sampled monthly from August 2007-July 2008 using three sampling methods: Shannon traps, CDC miniature light traps and Disney traps. Sampling was conducted for 3 h during three consecutive nights, beginning at sunset. A total of 4,387 sandflies were collected during the course of the study: 2,718 individuals in Finca Guadalupe Zajú, 605 in Finca Argovia and 1,064 in Teotihuacán del Valle. The Shannon traps captured 94.3% of the total sandflies, while the CDC light traps and Disney traps captured 4.9% and 0.8%, respectively. More females than males were collected at all sites. While the number of sandflies captured was positively correlated with temperature and relative humidity, a negative correlation was observed between sandfly numbers and rainfall. Five species of sandflies were captured: Lutzomyia cruciata , Lutzomyia texana , Lutzomyia ovallesi , Lutzomyia cratifer / undulata and Brumptomyia sp. Lu. cruciata , constituting 98.8% of the total, was the most abundant species. None of the captured sandflies was infected with Leishmania spp. PMID:24271002

  9. Eimeria Oocyst Concentrations and Species Composition in Litter from Commercial Broiler Farms During Anticoccidial Drug or Live Eimeria Oocyst Vaccine Control Programs.

    PubMed

    Jenkins, Mark C; Parker, Carolyn; Ritter, Donald

    2017-06-01

    The purpose of this study was to determine if Eimeria oocyst concentrations and species composition in commercial broiler house litter changed during different cycles of anticoccidial drug (ACD) or live Eimeria oocyst vaccine (VAC) control programs and if there was a correlation between Eimeria oocyst levels and broiler performance. Litter samples were collected from a total of 15 different broiler farms encompassing a total of 45 individual houses during at least one complete grow-out cycle over a 21-mo period. Of these 15 broiler farms, three were followed for the entire 21-mo period spanning three ACD and four VAC cycles. Samples were collected at 2, 4, and 7-8 wk of grow-out corresponding to starter, grower, and withdraw periods of the ACD cycle. On a number of occasions, litter samples were obtained just prior to chick placement. Eimeria oocysts were isolated from all samples, counted by microscopy, and extracted for DNA to identify Eimeria species by ITS1 PCR. In general, Eimeria oocyst concentration in litter reached peak levels at 2-4 wk of grow-out regardless of coccidiosis control measure being used. However, peak oocyst numbers were sometimes delayed until 7-8 wk, indicating some level of Eimeria spp. drug resistance or incomplete vaccine coverage. Eimeria maxima , Eimeria acervulina , Eimeria praecox, and Eimeria tenella were generally present in all samples, and no difference in the species composition was noted between houses on a particular farm. While Eimeria species composition was similar among houses, Eimeria spp. oocyst levels exhibited sporadic peaks in one house of a given location's houses. Of particular interest was the observed correlation between E. maxima oocyst abundance and chick mortality. However, no correlation was observed in E. maxima oocyst levels, and the performance parameters adjusted feed conversion ratio and average daily weight gain. This study showed that understanding the dynamics of Eimeria spp. oocyst levels and species

  10. Post-fire salvage logging alters species composition and reduces cover, richness, and diversity in Mediterranean plant communities.

    PubMed

    Leverkus, Alexandro B; Lorite, Juan; Navarro, Francisco B; Sánchez-Cañete, Enrique P; Castro, Jorge

    2014-01-15

    An intense debate exists on the effects of post-fire salvage logging on plant community regeneration, but scant data are available derived from experimental studies. We analyzed the effects of salvage logging on plant community regeneration in terms of species richness, diversity, cover, and composition by experimentally managing a burnt forest on a Mediterranean mountain (Sierra Nevada, S Spain). In each of three plots located at different elevations, three replicates of three treatments were implemented seven months after the fire, differing in the degree of intervention: "Non-Intervention" (all trees left standing), "Partial Cut plus Lopping" (felling 90% of the trees, cutting the main branches, and leaving all the biomass in situ), and "Salvage Logging" (felling and piling the logs, and masticating the woody debris). Plant composition in each treatment was monitored two years after the fire in linear point transects. Post-fire salvage logging was associated with reduced species richness, Shannon diversity, and total plant cover. Moreover, salvaged sites hosted different species assemblages and 25% lower cover of seeder species (but equal cover of resprouters) compared to the other treatments. Cover of trees and shrubs was also lowest in Salvage Logging, which could suggest a potential slow-down of forest regeneration. Most of these results were consistent among the three plots despite plots hosting different plant communities. Concluding, our study suggests that salvage logging may reduce species richness and diversity, as well as the recruitment of woody species, which could delay the natural regeneration of the ecosystem. Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. Interactive effects between plant functional types and soil factors on tundra species diversity and community composition.

    PubMed

    Iturrate-Garcia, Maitane; O'Brien, Michael J; Khitun, Olga; Abiven, Samuel; Niklaus, Pascal A; Schaepman-Strub, Gabriela

    2016-11-01

    Plant communities are coupled with abiotic factors, as species diversity and community composition both respond to and influence climate and soil characteristics. Interactions between vegetation and abiotic factors depend on plant functional types (PFT) as different growth forms will have differential responses to and effects on site characteristics. However, despite the importance of different PFT for community assembly and ecosystem functioning, research has mainly focused on vascular plants. Here, we established a set of observational plots in two contrasting habitats in northeastern Siberia in order to assess the relationship between species diversity and community composition with soil variables, as well as the relationship between vegetation cover and species diversity for two PFT (nonvascular and vascular). We found that nonvascular species diversity decreased with soil acidity and moisture and, to a lesser extent, with soil temperature and active layer thickness. In contrast, no such correlation was found for vascular species diversity. Differences in community composition were found mainly along soil acidity and moisture gradients. However, the proportion of variation in composition explained by the measured soil variables was much lower for nonvascular than for vascular species when considering the PFT separately. We also found different relationships between vegetation cover and species diversity according the PFT and habitat. In support of niche differentiation theory, species diversity and community composition were related to edaphic factors. The distinct relationships found for nonvascular and vascular species suggest the importance of considering multiple PFT when assessing species diversity and composition and their interaction with edaphic factors. Synthesis : Identifying vegetation responses to edaphic factors is a first step toward a better understanding of vegetation-soil feedbacks under climate change. Our results suggest that incorporating

  12. Are cattle surrogate wildlife? Savanna plant community composition explained by total herbivory more than herbivore type.

    PubMed

    Veblen, Kari E; Porensky, Lauren M; Riginos, Corinna; Young, Truman P

    2016-09-01

    The widespread replacement of wild ungulate herbivores by domestic livestock in African savannas is composed of two interrelated phenomena: (1) loss or reduction in numbers of individual wildlife species or guilds and (2) addition of livestock to the system. Each can have important implications for plant community dynamics. Yet very few studies have experimentally addressed the individual, combined, and potentially interactive effects of wild vs. domestic herbivore species on herbaceous plant communities within a single system. Additionally, there is little information about whether, and in which contexts, livestock might functionally replace native herbivore wildlife or, alternatively, have fundamentally different effects on plant species composition. The Kenya Long-term Exclosure Experiment, which has been running since 1995, is composed of six treatment combinations of mega-herbivores, meso-herbivore ungulate wildlife, and cattle. We sampled herbaceous vegetation 25 times between 1999 and 2013. We used partial redundancy analysis and linear mixed models to assess effects of herbivore treatments on overall plant community composition and key plant species. Plant communities in the six different herbivore treatments shifted directionally over time and diverged from each other substantially by 2013. Plant community composition was strongly related (R 2  = 0.92) to residual plant biomass, a measure of herbivore utilization. Addition of any single herbivore type (cattle, wildlife, or mega-herbivores) caused a shift in plant community composition that was proportional to its removal of plant biomass. These results suggest that overall herbivory pressure, rather than herbivore type or complex interactions among different herbivore types, was the main driver of changes in plant community composition. Individual plant species, however, did respond most strongly to either wild ungulates or cattle. Although these results suggest considerable functional similarity between

  13. Dispersal rates affect species composition in metacommunities of Sarracenia purpurea inquilines.

    PubMed

    Kneitel, Jamie M; Miller, Thomas E

    2003-08-01

    Dispersal among local communities can have a variety of effects on species composition and diversity at local and regional scales. Local conditions (e.g., resource and predator densities) can have independent effects, as well as interact with dispersal, to alter these patterns. Based on metacommunity models, we predicted that local diversity would show a unimodal relationship with dispersal frequency. We manipulated dispersal frequencies, resource levels, and the presence of predators (mosquito larvae) among communities found in the water-filled leaves of the pitcher plant Sarracenia purpurea. Diversity and abundance of species of the middle trophic level, protozoa and rotifers, were measured. Increased dispersal frequencies significantly increased regional species richness and protozoan abundance while decreasing the variance among local communities. Dispersal frequency interacted with predation at the local community scale to produce patterns of diversity consistent with the model. When predators were absent, we found a unimodal relationship between dispersal frequency and diversity, and when predators were present, there was a flat relationship. Intermediate dispersal frequencies maintained some species in the inquiline communities by offsetting extinction rates. Local community composition and the degree of connectivity between communities are both important for understanding species diversity patterns at local and regional scales.

  14. Comparison of the Proximate Composition, Total Carotenoids and Total Polyphenol Content of Nine Orange-Fleshed Sweet Potato Varieties Grown in Bangladesh.

    PubMed

    Alam, Mohammad Khairul; Rana, Ziaul Hasan; Islam, Sheikh Nazrul

    2016-09-14

    In an attempt to develop the food composition table for Bangladesh, the nutritional composition of nine varieties of orange-fleshed sweet potato was analyzed together with total carotenoids (TCC) and total polyphenol content (TPC). Each variety showed significant variation in different nutrient contents. The quantification of the TCC and TPC was done by spectrophotometric measurement, and the proximate composition was done by the AOAC method. The obtained results showed that total polyphenol content varied from 94.63 to 136.05 mg gallic acid equivalent (GAE)/100 g fresh weight. Among the selected sweet potatoes, Bangladesh Agricultural Research Institute (BARI) Sweet Potato 7 (SP7) contained the highest, whereas BARI SP6 contained the lowest amount of total polyphenol content. The obtained results also revealed that total carotenoids content ranged from 0.38 to 7.24 mg/100 g fresh weight. BARI SP8 showed the highest total carotenoids content, whereas BARI SP6 showed the lowest. Total carotenoids content was found to be higher in dark orange-colored flesh varieties than their light-colored counterparts. The results of the study indicated that selected sweet potato varieties are rich in protein and carbohydrate, low in fat, high in polyphenol and carotenoids and, thus, could be a good source of dietary antioxidants to prevent free radical damage, which leads to chronic diseases, and also to prevent vitamin A malnutrition.

  15. Comparison of the Proximate Composition, Total Carotenoids and Total Polyphenol Content of Nine Orange-Fleshed Sweet Potato Varieties Grown in Bangladesh

    PubMed Central

    Alam, Mohammad Khairul; Rana, Ziaul Hasan; Islam, Sheikh Nazrul

    2016-01-01

    In an attempt to develop the food composition table for Bangladesh, the nutritional composition of nine varieties of orange-fleshed sweet potato was analyzed together with total carotenoids (TCC) and total polyphenol content (TPC). Each variety showed significant variation in different nutrient contents. The quantification of the TCC and TPC was done by spectrophotometric measurement, and the proximate composition was done by the AOAC method. The obtained results showed that total polyphenol content varied from 94.63 to 136.05 mg gallic acid equivalent (GAE)/100 g fresh weight. Among the selected sweet potatoes, Bangladesh Agricultural Research Institute (BARI) Sweet Potato 7 (SP7) contained the highest, whereas BARI SP6 contained the lowest amount of total polyphenol content. The obtained results also revealed that total carotenoids content ranged from 0.38 to 7.24 mg/100 g fresh weight. BARI SP8 showed the highest total carotenoids content, whereas BARI SP6 showed the lowest. Total carotenoids content was found to be higher in dark orange-colored flesh varieties than their light-colored counterparts. The results of the study indicated that selected sweet potato varieties are rich in protein and carbohydrate, low in fat, high in polyphenol and carotenoids and, thus, could be a good source of dietary antioxidants to prevent free radical damage, which leads to chronic diseases, and also to prevent vitamin A malnutrition. PMID:28231159

  16. Species composition of developing Central Appalachian hardwood stands following clearcutting

    Treesearch

    Lance A. Vickers; Thomas Fox

    2015-01-01

    This study examined the species composition of 47 paired stands on submesic sites on the Appalachian Plateau of West Virginia. Paired stands consisted of a mature stand adjacent to a young clearcut that was

  17. Greenhouse gas emissions from dung pats vary with dung beetle species and with assemblage composition

    PubMed Central

    Arnieri, Fabrizio; Caprio, Enrico; Nervo, Beatrice; Pelissetti, Simone; Palestrini, Claudia; Roslin, Tomas; Rolando, Antonio

    2017-01-01

    Cattle farming is a major source of greenhouse gases (GHGs). Recent research suggests that GHG fluxes from dung pats could be affected by biotic interactions involving dung beetles. Whether and how these effects vary among beetle species and with assemblage composition is yet to be established. To examine the link between GHGs and different dung beetle species assemblages, we used a closed chamber system to measure fluxes of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) from cattle dung pats. Targeting a total of four dung beetle species (a pat-dwelling species, a roller of dung balls, a large and a small tunnelling species), we ran six experimental treatments (four monospecific and two mixed) and two controls (one with dung but without beetles, and one with neither dung nor beetles). In this setting, the overall presence of beetles significantly affected the gas fluxes, but different species contributed unequally to GHG emissions. When compared to the control with dung, we detected an overall reduction in the total cumulative CO2 flux from all treatments with beetles and a reduction in N2O flux from the treatments with the three most abundant dung beetle species. These reductions can be seen as beneficial ecosystem services. Nonetheless, we also observed a disservice provided by the large tunneler, Copris lunaris, which significantly increased the CH4 flux–an effect potentially traceable to the species’ nesting strategy involving the construction of large brood balls. When fluxes were summed into CO2-equivalents across individual GHG compounds, dung with beetles proved to emit less GHGs than did beetle-free dung, with the mix of the three most abundant species providing the highest reduction (-32%). As the mix of multiple species proved the most effective in reducing CO2-equivalents, the conservation of diverse assemblages of dung beetles emerges as a priority in agro-pastoral ecosystems. PMID:28700590

  18. Frog species richness, composition and beta-diversity in coastal Brazilian restinga habitats.

    PubMed

    Rocha, C F D; Hatano, F H; Vrcibradic, D; Van Sluys, M

    2008-02-01

    We studied the species richness and composition of frogs in 10 restinga habitats (sand dune environments dominated by herbaceous and shrubby vegetation) along approximately 1500 km of coastal areas of three Brazilian States: Rio de Janeiro (Grumari, Maricá, Massambaba, Jurubatiba and Grussaí), Espírito Santo (Praia das Neves and Setiba) and Bahia (Prado and Trancoso). We estimated beta-diversity and similarity among areas and related these parameters to geographic distance between areas. All areas were surveyed with a similar sampling procedure. We found 28 frog species belonging to the families Hylidae, Microhylidae, Leptodactylidae and Bufonidae. Frogs in restingas were in general nocturnal with no strictly diurnal species. The richest restinga was Praia das Neves (13 species), followed by Grussaí and Trancoso (eight species in each). The commonest species in the restingas was Scinax alter (found in eight restingas), followed by Aparasphenodon brunoi (seven areas). Our data shows that richness and composition of frog communities vary consistently along the eastern Brazilian coast and, in part, the rate of species turnover is affected by the distance among areas. Geographic distance explained approximately 12% of species turnover in restingas and about 9.5% of similarity among frog assemblages. Although geographic distance somewhat affects frog assemblages, other factors (e.g. historical factors, disturbances) seem to be also involved in explaining present frog assemblage composition in each area and species turnover among areas. The frog fauna along restinga habitats was significantly nested (matrix community temperature = 26.13 degrees; p = 0.007). Our data also showed that the most hospitable restinga was Praia das Neves and indicated that this area should be protected as a conservation unit. Frog assemblage of each area seems to partially represent a nested subset of the original assemblage, although we should not ignore the importance of historical

  19. Phylogeography above the species level for perennial species in a composite genus

    PubMed Central

    Tremetsberger, Karin; Ortiz, María Ángeles; Terrab, Anass; Balao, Francisco; Casimiro-Soriguer, Ramón; Talavera, María; Talavera, Salvador

    2016-01-01

    In phylogeography, DNA sequence and fingerprint data at the population level are used to infer evolutionary histories of species. Phylogeography above the species level is concerned with the genealogical aspects of divergent lineages. Here, we present a phylogeographic study to examine the evolutionary history of a western Mediterranean composite, focusing on the perennial species of Helminthotheca (Asteraceae, Cichorieae). We used molecular markers (amplified fragment length polymorphism (AFLP), internal transcribed spacer and plastid DNA sequences) to infer relationships among populations throughout the distributional range of the group. Interpretation is aided by biogeographic and molecular clock analyses. Four coherent entities are revealed by Bayesian mixture clustering of AFLP data, which correspond to taxa previously recognized at the rank of subspecies. The origin of the group was in western North Africa, from where it expanded across the Strait of Gibraltar to the Iberian Peninsula and across the Strait of Sicily to Sicily. Pleistocene lineage divergence is inferred within western North Africa as well as within the western Iberian region. The existence of the four entities as discrete evolutionary lineages suggests that they should be elevated to the rank of species, yielding H. aculeata, H. comosa, H. maroccana and H. spinosa, whereby the latter two necessitate new combinations. PMID:26644340

  20. Molecular species composition of plant cardiolipin determined by liquid chromatography mass spectrometry

    PubMed Central

    Zhou, Yonghong; Peisker, Helga

    2016-01-01

    Cardiolipin (CL), an anionic phospholipid of the inner mitochondrial membrane, provides essential functions for stabilizing respiratory complexes and is involved in mitochondrial morphogenesis and programmed cell death in animals. The role of CL and its metabolism in plants are less well understood. The measurement of CL in plants, including its molecular species composition, is hampered by the fact that CL is of extremely low abundance, and that plants contain large amounts of interfering compounds including galactolipids, neutral lipids, and pigments. We used solid phase extraction by anion exchange chromatography to purify CL from crude plant lipid extracts. LC/MS was used to determine the content and molecular species composition of CL. Thus, up to 23 different molecular species of CL were detected in different plant species, including Arabidopsis, mung bean, spinach, barley, and tobacco. Similar to animals, plant CL is dominated by highly unsaturated species, mostly containing linoleic and linolenic acid. During phosphate deprivation or exposure to an extended dark period, the amount of CL decreased in Arabidopsis, accompanied with an increased degree in unsaturation. The mechanism of CL remodeling during stress, and the function of highly unsaturated CL molecular species, remains to be defined. PMID:27179363

  1. Bumble Bees (Hymenoptera: Apidae: Bombus spp.) of Interior Alaska: Species Composition, Distribution, Seasonal Biology, and Parasites

    PubMed Central

    Pampell, Rehanon; Pantoja, Alberto; Holloway, Patricia; Knight, Charles; Ranft, Richard

    2015-01-01

    Abstract Background Despite the ecological and agricultural significance of bumble bees in Alaska, very little is known and published about this important group at the regional level. The objectives of this study were to provide baseline data on species composition, distribution, seasonal biology, and parasites of the genus Bombus at three major agricultural locations within Alaska: Fairbanks, Delta Junction, and Palmer, to lay the groundwork for future research on bumble bee pollination in Alaska. New information A total of 8,250 bumble bees representing 18 species was collected from agricultural settings near Delta Junction, Fairbanks, and Palmer, Alaska in 2009 and 2010. Of the 8,250 specimens, 51% were queens, 32.7% were workers, and 16.2% were males. The species composition and relative abundances varied among sites and years. Delta Junction had the highest relative abundance of bumble bees, representing 51.6% of the specimens collected; the other two locations, Fairbanks and Palmer represented 26.5% and 21.8% of the overall catch respectively. The species collected were: Bombus bohemicus Seidl 1837 (= B. ashtoni (Cresson 1864)), B. balteatus Dahlbom 1832, B. bifarius Cresson 1878, B. centralis Cresson 1864, B. cryptarum (Fabricius 1775) (=B. moderatus Cresson 1863), B. distinguendus Morawitz 1869, B. flavidus Eversmann 1852 (=B. fernaldae Franklin 1911), B. flavifrons Cresson 1863, B. frigidus Smith 1854, B. insularis (Smith 1861), B. jonellus (Kirby 1802), B. melanopygus Nylander 1848, B. mixtus Cresson 1878, B. neoboreus Sladen 1919, B. occidentalis Greene 1858, B. perplexus Cresson 1863, B. rufocinctus Cresson 1863, and B. sylvicola Kirby 1837. Overall, the most common bumble bees near agricultural lands were B. centralis, B. frigidus, B. jonellus, B. melanopygus, B. mixtus, and B. occidentalis. Species' relative population densities and local diversity were highly variable from year to year. Bombus occidentalis, believed to be in decline in the Pacific

  2. Physiographic position, disturbance and species composition in North Carolina coastal plain forests

    Treesearch

    James G. Wyant; Ralph J. Alig; William A. Bechtold

    1991-01-01

    Relations among physiographic heterogeneity, disturbance and temporal change in forest composition were analyzed on 765 forest stands in the southern coastal plain of North Carolina. Physiographic position strongly restricted the species composition of forest stands, though broad overlap of some physiographic classes was noted. Forest stands in different physiographic...

  3. Improved fatty acid analysis of conjugated linoleic acid rich egg yolk triacylglycerols and phospholipid species.

    PubMed

    Shinn, Sara; Liyanage, Rohana; Lay, Jack; Proctor, Andrew

    2014-07-16

    Reports from chicken conjugated linoleic acid (CLA) feeding trials are limited to yolk total fatty acid composition, which consistently described increased saturated fatty acids and decreased monounsaturated fatty acids. However, information on CLA triacylglycerol (TAG) and phospholipid (PL) species is limited. This study determined the fatty acid composition of total lipids in CLA-rich egg yolk produced with CLA-rich soy oil, relative to control yolks using gas chromatography with flame ionization detection (GC-FID), determined TAG and PL fatty acid compositions by thin-layer chromatography-GC-FID (TLC-GC-FID), identified intact PL and TAG species by TLC-matrix-assisted laser desorption/ionization mass spectrometry (TLC-MALDI-MS), and determined the composition of TAG and PL species in CLA and control yolks by direct flow infusion electrospray ionization MS (DFI ESI-MS). In total, 2 lyso-phosphatidyl choline (LPC) species, 1 sphingomyelin species, 17 phosphatidyl choline species, 19 TAG species, and 9 phosphatidyl ethanolamine species were identified. Fifty percent of CLA was found in TAG, occurring predominantly in C52:5 and C52:4 TAG species. CLA-rich yolks contained significantly more LPC than did control eggs. Comprehensive lipid profiling may provide insight on relationships between lipid composition and the functional properties of CLA-rich eggs.

  4. Fatty Acid Compositions of Six Wild Edible Mushroom Species

    PubMed Central

    Günç Ergönül, Pelin; Akata, Ilgaz; Kalyoncu, Fatih; Ergönül, Bülent

    2013-01-01

    The fatty acids of six wild edible mushroom species (Boletus reticulatus, Flammulina velutipes var. velutipes, Lactarius salmonicolor, Pleurotus ostreatus, Polyporus squamosus, and Russula anthracina) collected from different regions from Anatolia were determined. The fatty acids were identified and quantified by gas chromatography and studied using fruit bodies. Fatty acid composition varied among species. The dominant fatty acid in fruit bodies of all mushrooms was cis-linoleic acid (18 : 2). Percentage of cis-linoleic acid in species varied from 22.39% to 65.29%. The other major fatty acids were, respectively, cis-oleic, palmitic, and stearic acids. Fatty acids analysis of the mushrooms showed that the unsaturated fatty acids were at higher concentrations than saturated fatty acids. PMID:23844377

  5. Species composition and natural infectivity of anthropophilic Anopheles (Diptera: Culicidae) in Córdoba and Antioquia states in northwestern Colombia

    PubMed Central

    Gutiérrez, Lina A; González, John J; Gómez, Giovan F; Castro, Martha I; Rosero, Doris A; Luckhart, Shirley; Conn, Jan E; Correa, Margarita M

    2011-01-01

    Malaria is a serious health problem in Córdoba and Antioquia states in northwestern Colombia, where 64.4% of the total Colombian cases were reported in 2007. Because little entomological information is available in this region, the aim of this work was to identify the Anopheles species composition and natural infectivity of mosquitoes distributed in seven localities with the highest malaria transmission. A total of 1,768 Anopheles mosquitoes were collected using human landing catches from March 2007 to July 2008. Ten species were identified; overall, An. nuneztovari s.l. was the most widespread (62%) and showed the highest average human biting rates. There were six other species of the Nyssorhynchus subgenus: An. albimanus (11.6%), An. darlingi (9.8%), An. braziliensis (6.6%), An. triannulatus s.l. (3.5%), An. albitarsis s.l. and An. oswaldoi s.l. at <1%; and three of the Anopheles subgenus: An. punctimacula, An. pseudopunctipennis s.l. and An. neomaculipalpus at <1% each. Two species from Córdoba, An. nuneztovari and An. darlingi, were detected naturally infected by Plasmodium vivax VK247 using ELISA and confirmed by nested PCR. All species were active indoors and outdoors. These results provide basic information for targeted vector control strategies in these localities. PMID:20140372

  6. Do temperate tree species diversity and identity influence soil microbial community function and composition?

    PubMed

    Khlifa, Rim; Paquette, Alain; Messier, Christian; Reich, Peter B; Munson, Alison D

    2017-10-01

    Studies of biodiversity-ecosystem function in treed ecosystems have generally focused on aboveground functions. This study investigates intertrophic links between tree diversity and soil microbial community function and composition. We examined how microbial communities in surface mineral soil responded to experimental gradients of tree species richness (SR), functional diversity (FD), community-weighted mean trait value (CWM), and tree identity. The site was a 4-year-old common garden experiment near Montreal, Canada, consisting of deciduous and evergreen tree species mixtures. Microbial community composition, community-level physiological profiles, and respiration were evaluated using phospholipid fatty acid (PLFA) analysis and the MicroResp ™ system, respectively. The relationship between tree species richness and glucose-induced respiration (GIR), basal respiration (BR), metabolic quotient (qCO 2 ) followed a positive but saturating shape. Microbial communities associated with species mixtures were more active (basal respiration [BR]), with higher biomass (glucose-induced respiration [GIR]), and used a greater number of carbon sources than monocultures. Communities associated with deciduous tree species used a greater number of carbon sources than those associated with evergreen species, suggesting a greater soil carbon storage capacity. There were no differences in microbial composition (PLFA) between monocultures and SR mixtures. The FD and the CWM of several functional traits affected both BR and GIR. In general, the CWM of traits had stronger effects than did FD, suggesting that certain traits of dominant species have more effect on ecosystem processes than does FD. Both the functions of GIR and BR were positively related to aboveground tree community productivity. Both tree diversity (SR) and identity (species and functional identity-leaf habit) affected soil microbial community respiration, biomass, and composition. For the first time, we identified

  7. Drivers of metacommunity structure diverge for common and rare Amazonian tree species.

    PubMed

    Bispo, Polyanna da Conceição; Balzter, Heiko; Malhi, Yadvinder; Slik, J W Ferry; Dos Santos, João Roberto; Rennó, Camilo Daleles; Espírito-Santo, Fernando D; Aragão, Luiz E O C; Ximenes, Arimatéa C; Bispo, Pitágoras da Conceição

    2017-01-01

    We analysed the flora of 46 forest inventory plots (25 m x 100 m) in old growth forests from the Amazonian region to identify the role of environmental (topographic) and spatial variables (obtained using PCNM, Principal Coordinates of Neighbourhood Matrix analysis) for common and rare species. For the analyses, we used multiple partial regression to partition the specific effects of the topographic and spatial variables on the univariate data (standardised richness, total abundance and total biomass) and partial RDA (Redundancy Analysis) to partition these effects on composition (multivariate data) based on incidence, abundance and biomass. The different attributes (richness, abundance, biomass and composition based on incidence, abundance and biomass) used to study this metacommunity responded differently to environmental and spatial processes. Considering standardised richness, total abundance (univariate) and composition based on biomass, the results for common species differed from those obtained for all species. On the other hand, for total biomass (univariate) and for compositions based on incidence and abundance, there was a correspondence between the data obtained for the total community and for common species. Our data also show that in general, environmental and/or spatial components are important to explain the variability in tree communities for total and common species. However, with the exception of the total abundance, the environmental and spatial variables measured were insufficient to explain the attributes of the communities of rare species. These results indicate that predicting the attributes of rare tree species communities based on environmental and spatial variables is a substantial challenge. As the spatial component was relevant for several community attributes, our results demonstrate the importance of using a metacommunities approach when attempting to understand the main ecological processes underlying the diversity of tropical forest

  8. Consistency of effects of tropical-forest disturbance on species composition and richness relative to use of indicator taxa.

    PubMed

    Stork, N E; Srivastava, D S; Eggleton, P; Hodda, M; Lawson, G; Leakey, R R B; Watt, A D

    2017-08-01

    Lawton et al. (1998) found, in a highly cited study, that the species richness of 8 taxa each responds differently to anthropogenic disturbance in Cameroon forests. Recent developments in conservation science suggest that net number of species is an insensitive measure of change and that understanding which species are affected by disturbance is more important. It is also recognized that all disturbance types are not equal in their effect on species and that grouping species according to function rather than taxonomy is more informative of responses of biodiversity to change. In a reanalysis of most of the original Cameroon data set (canopy and ground ants, termites, canopy beetles, nematodes, and butterflies), we focused on changes in species and functional composition rather than richness and used a more inclusive measure of forest disturbance based on 4 component drivers of change: years since disturbance, tree cover, soil compaction, and degree of tree removal. Effects of disturbance on compositional change were largely concordant between taxa. Contrary to Lawton et al.'s findings, species richness for most groups did not decline with disturbance level, providing support for the view that trends in species richness at local scales do not reflect the resilience of ecosystems to disturbance. Disturbance affected species composition more strongly than species richness for butterflies, canopy beetles, and litter ants. For these groups, disturbance caused species replacements rather than just species loss. Only termites showed effects of disturbance on species richness but not composition, indicating species loss without replacement. Although disturbance generally caused changes in composition, the strength of this relationship depended on the disturbance driver. Butterflies, litter ants, and nematodes were correlated with amount of tree cover, canopy beetles were most strongly correlated with time since disturbance, and termites were most strongly correlated with

  9. Hydrological and environmental variables outperform spatial factors in structuring species, trait composition, and beta diversity of pelagic algae.

    PubMed

    Wu, Naicheng; Qu, Yueming; Guse, Björn; Makarevičiūtė, Kristė; To, Szewing; Riis, Tenna; Fohrer, Nicola

    2018-03-01

    There has been increasing interest in algae-based bioassessment, particularly, trait-based approaches are increasingly suggested. However, the main drivers, especially the contribution of hydrological variables, of species composition, trait composition, and beta diversity of algae communities are less studied. To link species and trait composition to multiple factors (i.e., hydrological variables, local environmental variables, and spatial factors) that potentially control species occurrence/abundance and to determine their relative roles in shaping species composition, trait composition, and beta diversities of pelagic algae communities, samples were collected from a German lowland catchment, where a well-proven ecohydrological modeling enabled to predict long-term discharges at each sampling site. Both trait and species composition showed significant correlations with hydrological, environmental, and spatial variables, and variation partitioning revealed that the hydrological and local environmental variables outperformed spatial variables. A higher variation of trait composition (57.0%) than species composition (37.5%) could be explained by abiotic factors. Mantel tests showed that both species and trait-based beta diversities were mostly related to hydrological and environmental heterogeneity with hydrological contributing more than environmental variables, while purely spatial impact was less important. Our findings revealed the relative importance of hydrological variables in shaping pelagic algae community and their spatial patterns of beta diversities, emphasizing the need to include hydrological variables in long-term biomonitoring campaigns and biodiversity conservation or restoration. A key implication for biodiversity conservation was that maintaining the instream flow regime and keeping various habitats among rivers are of vital importance. However, further investigations at multispatial and temporal scales are greatly needed.

  10. Understanding how seasonality and shifts in species composition impact emission estimates in semi-arid ecosystems

    NASA Astrophysics Data System (ADS)

    Sparks, A. M.; Yokelson, R. J.; Smith, A. M.; Marshall, J. D.; Tinkham, W.

    2013-12-01

    The importance of wildland fire as a source of trace gas emissions to the atmosphere has been demonstrated in the scientific literature and through numerous NASA funded campaigns to further understand the drivers and impacts of these emissions (e.g., SAFARI 1992, SAFARI 2000, TRACE A, etc). Most studies quantify emissions using remotely sensed data through multiplying the area burned, the quantity of fuel combusted, and the emission factors of a given gas species (EFX, grams of gas, X, emitted per kilogram of fuel consumed). The latter is known to exhibit considerable uncertainty and indeed a prior study as part of NASA's SAFARI 2000 campaign highlighted a seasonal dependence of carbonaceous gas species emissions. In this study, rangeland grass and shrub species were collected periodically from northern Great Basin shrub-steppe ecosystems during the typical burn season and burned in a small-scale laboratory setup where major carbonaceous and nitrogenous emission species were monitored and measured. Preliminary results indicate that emission factors of several major gas species, including carbon monoxide and nitrogen oxides, vary considerably over the course of a season. Large differences in emission apportionment between the rangeland species also suggests that shifting vegetation composition (via replacement of native with invasive species) can have a significant influence on emissions from semi-arid ecosystems. Further development of this data could lead to an enhanced understanding of how emission factors vary seasonally and how total emissions change with major vegetation shifts in other ecosystems.

  11. Soil Disturbance as a Grassland Restoration Measure—Effects on Plant Species Composition and Plant Functional Traits

    PubMed Central

    Schnoor, Tim; Bruun, Hans Henrik; Olsson, Pål Axel

    2015-01-01

    Soil disturbance is recognized as an important driver of biodiversity in dry grasslands, and can therefore be implemented as a restoration measure. However, because community re-assembly following disturbance includes stochastic processes, a focus only on species richness or establishment success of particular species will not inform on how plant communities respond ecologically to disturbance. We therefore evaluated vegetation development following disturbance by quantifying species richness, species composition and functional trait composition. Degraded calcareous sandy grassland was subjected to experimental disturbance treatments (ploughing or rotavation), and the vegetation was surveyed during four subsequent years of succession. Treated plots were compared with control plots representing untreated grassland, as well as nearby plots characterized by plant communities representing the restoration target. Species richness and functional diversity both increased in response to soil disturbance, and rotavation, but not ploughing, had a persistent positive effect on the occurrence of specialist species of calcareous sandy grassland. However, no type of soil disturbance caused the plant species composition to develop towards the target vegetation. The disturbance had an immediate and large impact on the vegetation, but the vegetation developed rapidly back towards the control sites. Plant functional composition analysis indicated that the treatments created habitats different both from control sites and target sites. Community-weighted mean Ellenberg indicator values suggested that the observed plant community response was at least partially due to an increase in nitrogen and water availability following disturbance. This study shows that a mild type of disturbance, such as rotavation, may be most successful in promoting specialist species in calcareous sandy grassland, but that further treatments are needed to reduce nutrient availability. We conclude that a

  12. Soil disturbance as a grassland restoration measure-effects on plant species composition and plant functional traits.

    PubMed

    Schnoor, Tim; Bruun, Hans Henrik; Olsson, Pål Axel

    2015-01-01

    Soil disturbance is recognized as an important driver of biodiversity in dry grasslands, and can therefore be implemented as a restoration measure. However, because community re-assembly following disturbance includes stochastic processes, a focus only on species richness or establishment success of particular species will not inform on how plant communities respond ecologically to disturbance. We therefore evaluated vegetation development following disturbance by quantifying species richness, species composition and functional trait composition. Degraded calcareous sandy grassland was subjected to experimental disturbance treatments (ploughing or rotavation), and the vegetation was surveyed during four subsequent years of succession. Treated plots were compared with control plots representing untreated grassland, as well as nearby plots characterized by plant communities representing the restoration target. Species richness and functional diversity both increased in response to soil disturbance, and rotavation, but not ploughing, had a persistent positive effect on the occurrence of specialist species of calcareous sandy grassland. However, no type of soil disturbance caused the plant species composition to develop towards the target vegetation. The disturbance had an immediate and large impact on the vegetation, but the vegetation developed rapidly back towards the control sites. Plant functional composition analysis indicated that the treatments created habitats different both from control sites and target sites. Community-weighted mean Ellenberg indicator values suggested that the observed plant community response was at least partially due to an increase in nitrogen and water availability following disturbance. This study shows that a mild type of disturbance, such as rotavation, may be most successful in promoting specialist species in calcareous sandy grassland, but that further treatments are needed to reduce nutrient availability. We conclude that a

  13. Biochemical composition of three algal species proposed as food for captive freshwater mussels

    USGS Publications Warehouse

    Gatenby, C.M.; Orcutt, D.M.; Kreeger, D.A.; Parker, B.C.; Jones, V.A.; Neves, R.J.

    2003-01-01

    To identify potential diets for rearing captive freshwater mussels, the protein, carbohydrate (CHO), and lipid contents of two green algae, Neochloris oleoabundans, Bracteacoccus grandis, and one diatom, Phaeodactylum tricornutum, were compared at different growth stages. The fatty acid and sterol composition were also identified. Protein was greatest (55-70%) for all species at late log growth stage (LL), and declined in late stationary (LS) growth. CHO was greatest at LS stage for all species (33.9-56.4% dry wt). No significant change in lipid levels occurred with growth stage, but tended to increase in N. oleoabundans. Mean lipid content differed significantly in the order: N. oleoabundans > P. tricornutum > B. grandis. Total fatty acids (TFA) were higher at LS stage compared to other stages in the two green algae, and stationary stage in the diatom. Mean unsaturated fatty acids (UFA) as %TFA was significantly higher in N. oleoabundans than the other species. The green algae contained high percentages of C-18 polyunsaturated fatty acids (PUFAs), while the diatom was abundant in C-16 saturated and mono-unsaturated fatty acids and C-20 PUFA fatty acids. Growth stage had no effect on sterol concentration of any species. B. grandis showed significantly higher sterol levels than the other species except P. tricornutum at S stage. B. grandis was characterized by predominantly ??5, C-29 sterols, while N. oleoabundans synthesized ??5,7, ??5,7,22, and ??7, C-28 sterols. P. tricornutum produced primarily a ??5,22, C-28 sterol, and a small amount of a ??7,22, C-28 sterol.

  14. Regional variation in Caribbean dry forest tree species composition

    Treesearch

    Janet Franklin; Julie Ripplinger; Ethan H. Freid; Humfredo Marcano-Vega; David W. Steadman

    2015-01-01

    How does tree species composition vary in relation to geographical and environmental gradients in a globally rare tropical/subtropical broadleaf dry forest community in the Caribbean? We analyzed data from 153 Forest Inventory and Analysis (FIA) plots from Puerto Rico and the U.S. Virgin Islands (USVI), along with 42 plots that we sampled in the Bahamian Archipelago (...

  15. SPECIES COMPOSITION, DISTRIBUTION, LIFE FORMS AND FOLK NOMENCLATURE OF FOREST AND COMMON LAND PLANTS OF WESTERN CHITWAN, NEPAL

    PubMed Central

    Dangol, D. R.

    2012-01-01

    This paper enumerates 349 plant species belonging to 77 families of vascular plants collected in the winter seasons of 1996 and 2000 by the flora teams of the Population and Ecology Research Laboratory, Nepal. Of the total species, 249 species belong to dicotyledons, 87 species to monocotyledons and 13 species to pteridophytes. Among the families, dicotyledons contributed the highest number of families (55 in number) followed by monocotyledons and pteridophytes. In the study areas, species composition varies with the type of habitats in the study plots. Some species are unique in distribution. The highest unique species are contributed by common lands (87 spp.), followed by the Chitwan National Park forest (36 spp.) and Tikauli forest (32 spp.). Ageratum houstonianum Mill., Cynodon dactylon (L.) Pers., Imperata cylindrica (L.) Beauv., Rungia parviflora (Retz.) Nees, Saccharum spontaneum L. and Thelypteris auriculata (J. Sm.) K. Iwats are the most common species across all the research blocks. Of the listed plants, many plants have local names either in Nepalese or other tribal languages. Plants are named in different ways on the basis of habit, habitat, smell, taste, and morphological characters of the plants, which are also the basis of nomenclature in plant taxonomy. PMID:22962539

  16. Logging impacts on avian species richness and composition differ across latitudes and foraging and breeding habitat preferences.

    PubMed

    LaManna, Joseph A; Martin, Thomas E

    2017-08-01

    Understanding the causes underlying changes in species diversity is a fundamental pursuit of ecology. Animal species richness and composition often change with decreased forest structural complexity associated with logging. Yet differences in latitude and forest type may strongly influence how species diversity responds to logging. We performed a meta-analysis of logging effects on local species richness and composition of birds across the world and assessed responses by different guilds (nesting strata, foraging strata, diet, and body size). This approach allowed identification of species attributes that might underlie responses to this anthropogenic disturbance. We only examined studies that allowed forests to regrow naturally following logging, and accounted for logging intensity, spatial extent, successional regrowth after logging, and the change in species composition expected due to random assembly from regional species pools. Selective logging in the tropics and clearcut logging in temperate latitudes caused loss of species from nearly all forest strata (ground to canopy), leading to substantial declines in species richness (up to 27% of species). Few species were lost or gained following any intensity of logging in lower-latitude temperate forests, but the relative abundances of these species changed substantially. Selective logging at higher-temperate latitudes generally replaced late-successional specialists with early-successional specialists, leading to no net changes in species richness but large changes in species composition. Removing less basal area during logging mitigated the loss of avian species from all forests and, in some cases, increased diversity in temperate forests. This meta-analysis provides insights into the important role of habitat specialization in determining differential responses of animal communities to logging across tropical and temperate latitudes. © 2016 Cambridge Philosophical Society.

  17. Composition and species diversity of pine-wiregrass savannas of the Green Swamp, North Carolina

    Treesearch

    Joan Walker; Robert K. Peet

    1983-01-01

    Fire-maintained, species-rich pines wiregrass savannas in the Green Swamp, North Carolina were sampled over their natural range of environmental conditions and fire frequencies. Species composition, species richness, diversity (Exp H', I/ C), and aboveground production were documented and fertilization experiments conducted to assess possible mechanisms for the...

  18. Variation in contents of total phenolics and flavonoids and antioxidant activities in the leaves of 11 Eriobotrya species.

    PubMed

    Hong, Yanping; Lin, Shunquan; Jiang, Yueming; Ashraf, Muhammad

    2008-12-01

    Eriobotrya plants are known to have significant amounts of phenolics and flavonoids, and exhibit a strong antioxidant activity. Experiments were conducted to examine variation in the contents of total phenolics and flavonoids, and antioxidant activities in the leaves of 11 Eriobotrya species (Tibet loquat, Daduhe loquat, Hengchun loquat, Taiwan loquat, Oak leaf loquat, Bengal loquat, Fragrant loquat, Guangxi loquat, Obovate loquat, Big flower loquat, and common loquat, the last species include two materials, one is a cultivar 'Zaozhong 6', another is a wild tree). In these species, 'Zaozhong 6' loquat is a cultivar. The leaf extracts of 'Tibet', 'Obovate', 'Taiwan', 'Bengal' and 'Hengchun' loquats exhibited significantly higher contents of total flavonoids and total phenolics, compared with those of other species. Of these 11 species, the highest contents of total phenolics and total flavonoids were observed in 'Tibet' and 'Obovatae' loquats, respectively. The significantly stronger antioxidant abilities assessed by the DPPH radical scavenging activity and reducing power were obtained in the leaf extracts of 'Taiwan', 'Tibet', 'Bengal', 'Oak leaf', 'Hengchun' and 'Obovate' loquats, compared with the other species. In addition, significant correlations were found between the contents of total phenolics or flavonoids and DPPH radical scavenging activity/reducing power. This work indicates that the leaf extracts of the wild Eriobotrya species, 'Tibet', 'Obovatae', 'Taiwan', 'Bengal', 'Oak leaf' and 'Hengchun' loquats, exhibited significantly higher levels of total phenolics and flavonoids, and significantly stronger antioxidant activities, compared with the cultivated species, 'Zaozhong 6' loquat, which suggests that these wild species have a better utilization value.

  19. Plant Species Rather Than Climate Greatly Alters the Temporal Pattern of Litter Chemical Composition During Long-Term Decomposition

    NASA Astrophysics Data System (ADS)

    Li, Yongfu; Chen, Na; Harmon, Mark E.; Li, Yuan; Cao, Xiaoyan; Chappell, Mark A.; Mao, Jingdong

    2015-10-01

    A feedback between decomposition and litter chemical composition occurs with decomposition altering composition that in turn influences the decomposition rate. Elucidating the temporal pattern of chemical composition is vital to understand this feedback, but the effects of plant species and climate on chemical changes remain poorly understood, especially over multiple years. In a 10-year decomposition experiment with litter of four species (Acer saccharum, Drypetes glauca, Pinus resinosa, and Thuja plicata) from four sites that range from the arctic to tropics, we determined the abundance of 11 litter chemical constituents that were grouped into waxes, carbohydrates, lignin/tannins, and proteins/peptides using advanced 13C solid-state NMR techniques. Decomposition generally led to an enrichment of waxes and a depletion of carbohydrates, whereas the changes of other chemical constituents were inconsistent. Inconsistent convergence in chemical compositions during decomposition was observed among different litter species across a range of site conditions, whereas one litter species converged under different climate conditions. Our data clearly demonstrate that plant species rather than climate greatly alters the temporal pattern of litter chemical composition, suggesting the decomposition-chemistry feedback varies among different plant species.

  20. Plant Species Rather Than Climate Greatly Alters the Temporal Pattern of Litter Chemical Composition During Long-Term Decomposition

    PubMed Central

    Li, Yongfu; Chen, Na; Harmon, Mark E.; Li, Yuan; Cao, Xiaoyan; Chappell, Mark A.; Mao, Jingdong

    2015-01-01

    A feedback between decomposition and litter chemical composition occurs with decomposition altering composition that in turn influences the decomposition rate. Elucidating the temporal pattern of chemical composition is vital to understand this feedback, but the effects of plant species and climate on chemical changes remain poorly understood, especially over multiple years. In a 10-year decomposition experiment with litter of four species (Acer saccharum, Drypetes glauca, Pinus resinosa, and Thuja plicata) from four sites that range from the arctic to tropics, we determined the abundance of 11 litter chemical constituents that were grouped into waxes, carbohydrates, lignin/tannins, and proteins/peptides using advanced 13C solid-state NMR techniques. Decomposition generally led to an enrichment of waxes and a depletion of carbohydrates, whereas the changes of other chemical constituents were inconsistent. Inconsistent convergence in chemical compositions during decomposition was observed among different litter species across a range of site conditions, whereas one litter species converged under different climate conditions. Our data clearly demonstrate that plant species rather than climate greatly alters the temporal pattern of litter chemical composition, suggesting the decomposition-chemistry feedback varies among different plant species. PMID:26515033

  1. [Species composition and diversity of soil mesofauna in the 'Holy Hills' fragmentary tropical rain forest of Xishuangbanna, China].

    PubMed

    Yang, X; Sha, L

    2001-04-01

    The species composition and diversity of soil mesofauna were examined in fragmented dry tropical seasonal rainforest of tow 'Holy Hills' of Dai nationality, compared with the continuous moist tropical seasonal rain forest of Nature Reserve in Xishuangbanna area. 5 sample quadrats were selected along the diagonal of 20 m x 20 m sampling plot, and the samples of litterfall and 0-3 cm soil were collected from each 50 cm x 10 cm sample quadrat. Animals in soil sample were collected by using dry-funnel(Tullgren's), were identified to their groups according to the order. The H' index, D.G index and the pattern of relative abundance of species were used to compare the diversity of soil mesofauna. The results showed that the disturbance of vegetation and soil resulted by tropical rainforest fragmentation was the major factor affecting the diversity of soil mesofauna. Because the fragmented forest was intruded by some pioneer tree species and the "dry and warm" effect operated, this forest had more litterfall on the floor and more humus in the soil than the continuous moist rain forest. The soil condition with more soil organic matter, total N and P, higher pH value and lower soil bulk density became more favorable to the soil mesofauna. Therefore, the species richness, abundance and diversity of soil mesofauna in fragmented forests were higher than those in continuous forest, but the similarity of species composition in fragmented forest to the continuous forest was minimal. Soil mesofauna diversity in fragmented forests did not change with decreasing fragmented area, indicating that there was no species-area effect operation in this forest. The pattern of relative abundance of species in these forest soils was logarithmic series distribution.

  2. A decade of predatory control of zooplankton species composition of Lake Michigan

    USGS Publications Warehouse

    Makarewicz, Joseph C.; Bertram, Paul; Lewis, Theodore; Brown, Edward H.

    1995-01-01

    From 1983 to 1992, 71 species representing 38 genera from the Calanoida, Cladocera, Cyclopoida, Mysidacea, Rotifera, Mollusca and Harpacticoida comprised the offshore zooplankton community of Lake Michigan. Our data demonstrate that the composition and abundance of the calanoid community after 1983 is not unlike that of 1960s and that species diversity of the calanoid community is more diverse than the cladoceran community in the 1990s as compared to the early 1980s. Even though the relative biomass of the cladocerans has remained similar over the 1983-1993 period, the species diversity and evenness of the Cladocera community in the early 1990s is unlike anything that has been previously reported for Lake Michigan. Cladocera dominance is centered in one species, Daphnia galeata mendotae, and only three species of Cladocera were observed in the pelagic region of the lake in 1991 and 1992. Nutrient levels, phytoplankton biomass, and the abundance of planktivorous alewife and bloater chub and Bythotrephes are examined as possible causes of these changes in zooplankton species composition. The increase in Rotifera biomass, but not Crustacea, was correlated with an increase in relative biomass of unicellular algae. Food web models suggest Bythotrephes will cause Lake Michigan's plankton to return to a community similar to that of the 1970s; that is Diaptomus dominated. Such a change has occurred. However, correlational analysis suggest that alewife and bloater chubs (especially juveniles) are affecting size and biomass of larger species of zooplankton as well as Bythotrephes.

  3. Equisetum species show uniform epicuticular wax structures but diverse composition patterns

    PubMed Central

    Brune, Thomas; Haas, Klaus

    2011-01-01

    Background and aims Only few data on the epicuticular waxes (EWs) of horsetails are available. This contribution therefore focuses on the wax micromorphology and chemical composition of Equisetum species of the subgenera Equisetum and Hippochaete. Methodology Distribution patterns and structural details of EW on the shoots were studied by scanning electron microscopy. After extraction with chloroform, the chemical composition of wax isolates was analysed by gas chromatography. Principal results Epicuticular wax crystals were non-oriented platelets or membraneous platelets. They were usually located on subsidiary cells of stomata and adjacent cells. Other parts of the shoots were covered mainly with a smooth wax film or small granules only. The chemical constituents found were alkanes, esters, aldehydes, primary alcohols and free fatty acids in a range of C20–C36 (in esters C36–C56). All species of the subgenus Hippochaete showed a similar pattern of fractions with high percentages of alkanes and aldehydes, whereas the subgenus Equisetum species had distinctly different wax compositions. Extracts from the internodes—surfaces without well-developed EW crystals and only few stomata—showed the lowest contents of aldehydes. Conclusions The covering with EW crystals will provide unhindered gas exchange and, combined with intracuticular wax, may prevent excess water loss during winter in the evergreen shoots of the subgenus Hippochaete. The results indicate that the Equisetum wax micromorphology and biosynthesis are comparable to EW of other pteridophyte classes and mosses. PMID:22476480

  4. Tree cover and species composition effects on academic performance of primary school students.

    PubMed

    Sivarajah, Sivajanani; Smith, Sandy M; Thomas, Sean C

    2018-01-01

    Human exposure to green space and vegetation is widely recognized to result in physical and mental health benefits; however, to date, the specific effects of tree cover, diversity, and species composition on student academic performance have not been investigated. We compiled standardized performance scores in Grades 3 and 6 for the collective student body in 387 schools across the Toronto District School Board (TDSB), and examined variation in relation to tree cover, tree diversity, and tree species composition based on comprehensive inventories of trees on school properties combined with aerial-photo-based assessments of tree cover. Analyses accounted for variation due to socioeconomic factors using the learning opportunity index (LOI), a regional composite index of external challenges to learning that incorporates income and other factors, such as students with English as a second language. As expected, LOI had the greatest influence on student academic performance; however, the proportion of tree cover, as distinct from other types of "green space" such as grass, was found to be a significant positive predictor of student performance, accounting for 13% of the variance explained in a statistical model predicting mean student performance assessments. The effects of tree cover and species composition were most pronounced in schools that showed the highest level of external challenges, suggesting the importance of urban forestry investments in these schools.

  5. Tree cover and species composition effects on academic performance of primary school students

    PubMed Central

    Smith, Sandy M.; Thomas, Sean C.

    2018-01-01

    Human exposure to green space and vegetation is widely recognized to result in physical and mental health benefits; however, to date, the specific effects of tree cover, diversity, and species composition on student academic performance have not been investigated. We compiled standardized performance scores in Grades 3 and 6 for the collective student body in 387 schools across the Toronto District School Board (TDSB), and examined variation in relation to tree cover, tree diversity, and tree species composition based on comprehensive inventories of trees on school properties combined with aerial-photo-based assessments of tree cover. Analyses accounted for variation due to socioeconomic factors using the learning opportunity index (LOI), a regional composite index of external challenges to learning that incorporates income and other factors, such as students with English as a second language. As expected, LOI had the greatest influence on student academic performance; however, the proportion of tree cover, as distinct from other types of “green space” such as grass, was found to be a significant positive predictor of student performance, accounting for 13% of the variance explained in a statistical model predicting mean student performance assessments. The effects of tree cover and species composition were most pronounced in schools that showed the highest level of external challenges, suggesting the importance of urban forestry investments in these schools. PMID:29474503

  6. Nectar production dynamics and sugar composition in two Mucuna species (Leguminosae, Faboideae) with different specialized pollinators

    NASA Astrophysics Data System (ADS)

    Agostini, Kayna; Sazima, Marlies; Galetto, Leonardo

    2011-11-01

    Nectar is secreted in particular rhythms throughout the lifespan of a flower, which allows determining the nectar production dynamics. This paper compares nectar features in Mucuna japira and Mucuna urens describing: dynamics of nectar production, floral response to nectar removal, resorption, nectar sugar composition, and variation in nectar sugar composition. M. japira inflorescence bears 12-21 yellow flowers, which are in anthesis for 7 days, whereas M. urens inflorescence bears 36-54 greenish flowers, but only 1-3 flowers are in anthesis simultaneously that last one night. Nectar volume and sugar concentration were measured, and the amount of sugar was estimated. Qualitative and quantitative nectar sugar composition was determined. Both species had a constant nectar sugar concentration (ca. 10% for M. japira and ca. 16% for M. urens) and secreted high volumes of nectar (ca. 340 μl per flower for M. japira and 310 μl per flower for M. urens), during 5 days for M. japira and 6 h for M. urens, but after the first removal, i.e., when flower opening mechanism is triggered, nectar production stops immediately. Nectar resorption occurred in both species. Nectar sugar composition showed some similarities between the species. Variation in nectar sugar composition occurred in both species. The Mucuna species are dependent on their pollinators to produce fruits and seeds, and they have different strategies to promote the necessary interaction with birds or bats, especially related to nectar and flower characteristics.

  7. Nectar production dynamics and sugar composition in two Mucuna species (Leguminosae, Faboideae) with different specialized pollinators.

    PubMed

    Agostini, Kayna; Sazima, Marlies; Galetto, Leonardo

    2011-11-01

    Nectar is secreted in particular rhythms throughout the lifespan of a flower, which allows determining the nectar production dynamics. This paper compares nectar features in Mucuna japira and Mucuna urens describing: dynamics of nectar production, floral response to nectar removal, resorption, nectar sugar composition, and variation in nectar sugar composition. M. japira inflorescence bears 12-21 yellow flowers, which are in anthesis for 7 days, whereas M. urens inflorescence bears 36-54 greenish flowers, but only 1-3 flowers are in anthesis simultaneously that last one night. Nectar volume and sugar concentration were measured, and the amount of sugar was estimated. Qualitative and quantitative nectar sugar composition was determined. Both species had a constant nectar sugar concentration (ca. 10% for M. japira and ca. 16% for M. urens) and secreted high volumes of nectar (ca. 340 μl per flower for M. japira and 310 μl per flower for M. urens), during 5 days for M. japira and 6 h for M. urens, but after the first removal, i.e., when flower opening mechanism is triggered, nectar production stops immediately. Nectar resorption occurred in both species. Nectar sugar composition showed some similarities between the species. Variation in nectar sugar composition occurred in both species. The Mucuna species are dependent on their pollinators to produce fruits and seeds, and they have different strategies to promote the necessary interaction with birds or bats, especially related to nectar and flower characteristics.

  8. Comparison of the chemical compositions and nutritive values of various pumpkin (Cucurbitaceae) species and parts

    PubMed Central

    Kim, Mi Young; Kim, Eun Jin; Kim, Young-Nam; Choi, Changsun

    2012-01-01

    Pumpkins have considerable variation in nutrient contents depending on the cultivation environment, species, or part. In this study, the general chemical compositions and some bioactive components, such as tocopherols, carotenoids, and β-sitosterol, were analyzed in three major species of pumpkin (Cucurbitaceae pepo, C. moschata, and C. maxima) grown in Korea and also in three parts (peel, flesh, and seed) of each pumpkin species. C. maxima had significantly more carbohydrate, protein, fat, and fiber than C. pepo or C. moschata (P < 0.05). The moisture content as well as the amino acid and arginine contents in all parts of the pumpkin was highest in C. pepo. The major fatty acids in the seeds were palmitic, stearic, oleic, and linoleic acids. C. pepo and C. moschata seeds had significantly more γ-tocopherol than C. maxima, whose seeds had the highest β-carotene content. C. pepo seeds had significantly more β-sitosterol than the others. Nutrient compositions differed considerably among the pumpkin species and parts. These results will be useful in updating the nutrient compositions of pumpkin in the Korean food composition database. Additional analyses of various pumpkins grown in different years and in different areas of Korea are needed. PMID:22413037

  9. Relationships between pigment composition variation and reflectance for plant species from a coastal savannah in California

    NASA Technical Reports Server (NTRS)

    Ustin, Susan L.; Sanderson, Eric W.; Grossman, Yaffa; Hart, Quinn J.

    1993-01-01

    Advances in imaging spectroscopy have indicated that remotely sensed reflectance measurements of the plant canopy may be used to identify and qualify some classes of canopy biochemicals; however, the manner in which differences in biochemical compositions translate into differences is not well understood. Most frequently, multiple linear regression routines have been used to correlate narrow band reflectance values with measured biochemical concentrations. Although some success has been achieved with such methods for given data sets, the bands selected by multiple regression are not consistent between data sets, nor is it always clear what physical or biological basis underlies the correlation. To examine the relationship between biochemical concentration and leaf reflectance signal we chose to focus on the visible spectrum where the primary biochemical absorbances are due to photosynthetic pigments. Pigments provide a range of absorbance features, occur over a range of concentrations in natural samples, and are ecophysiologically important. Concentrations of chlorophyll, for example, have been strongly correlated to foliar nitrogen levels within a species and to photosynthetic capacity across many species. In addition pigments effectively absorb most of the photosynthetically active radiation between 400-700 nm, a spectral region for which silicon detectors have good signal/noise characteristics. Our strategy has been to sample a variety of naturally occurring species to measure leaf reflectance and pigment compositions. We hope to extend our understanding of pigment reflectance effects to interpret small overlapping absorbances of other biochemicals in the infrared region. For this reason, selected samples were also tested to determine total nitrogen, crude protein, cellulose, and lignin levels. Leaf reflectance spectra measured with AVIRIS bandwidths and wavelengths were compared between species and within species and for differences between seasons, for changes

  10. Successional change in species composition alters climate sensitivity of grassland productivity.

    PubMed

    Shi, Zheng; Lin, Yang; Wilcox, Kevin R; Souza, Lara; Jiang, Lifen; Jiang, Jiang; Jung, Chang Gyo; Xu, Xia; Yuan, Mengting; Guo, Xue; Wu, Liyou; Zhou, Jizhong; Luo, Yiqi

    2018-05-31

    Succession theory predicts altered sensitivity of ecosystem functions to disturbance (i.e., climate change) due to the temporal shift in plant community composition. However, empirical evidence in global change experiments is lacking to support this prediction. Here, we present findings from an 8-year long-term global change experiment with warming and altered precipitation manipulation (double and halved amount). First, we observed a temporal shift in species composition over 8 years, resulting in a transition from an annual C 3 -dominant plant community to a perennial C 4 -dominant plant community. This successional transition was independent of any experimental treatments. During the successional transition, the response of aboveground net primary productivity (ANPP) to precipitation addition magnified from neutral to +45.3%, while the response to halved precipitation attenuated substantially from -17.6% to neutral. However, warming did not affect ANPP in either state. The findings further reveal that the time-dependent climate sensitivity may be regulated by successional change in species composition, highlighting the importance of vegetation dynamics in regulating the response of ecosystem productivity to precipitation change. © 2018 John Wiley & Sons Ltd.

  11. Logging impacts on avian species richness and composition differ across latitudes relative to foraging and breeding habitat preferences

    USGS Publications Warehouse

    LaManna, Joseph A.; Martin, Thomas E.

    2017-01-01

    Understanding the causes underlying changes in species diversity is a fundamental pursuit of ecology. Animal species richness and composition often change with decreased forest structural complexity associated with logging. Yet differences in latitude and forest type may strongly influence how species diversity responds to logging. We performed a meta-analysis of logging effects on local species richness and composition of birds across the world and assessed responses by different guilds (nesting strata, foraging strata, diet, and body size). This approach allowed identification of species attributes that might underlie responses to this anthropogenic disturbance. We only examined studies that allowed forests to regrow naturally following logging, and accounted for logging intensity, spatial extent, successional regrowth after logging, and the change in species composition expected due to random assembly from regional species pools. Selective logging in the tropics and clearcut logging in temperate latitudes caused loss of species from nearly all forest strata (ground to canopy), leading to substantial declines in species richness (up to 27% of species). Few species were lost or gained following any intensity of logging in lower-latitude temperate forests, but the relative abundances of these species changed substantially. Selective logging at higher-temperate latitudes generally replaced late-successional specialists with early-successional specialists, leading to no net changes in species richness but large changes in species composition. Removing less basal area during logging mitigated the loss of avian species from all forests and, in some cases, increased diversity in temperate forests. This meta-analysis provides insights into the important role of habitat specialization in determining differential responses of animal communities to logging across tropical and temperate latitudes.

  12. Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization.

    PubMed

    Kwiatkowski, M; Wurlitzer, M; Krutilin, A; Kiani, P; Nimer, R; Omidi, M; Mannaa, A; Bussmann, T; Bartkowiak, K; Kruber, S; Uschold, S; Steffen, P; Lübberstedt, J; Küpker, N; Petersen, H; Knecht, R; Hansen, N O; Zarrine-Afsar, A; Robertson, W D; Miller, R J D; Schlüter, H

    2016-02-16

    Posttranslational modifications and proteolytic processing regulate almost all physiological processes. Dysregulation can potentially result in pathologic protein species causing diseases. Thus, tissue species proteomes of diseased individuals provide diagnostic information. Since the composition of tissue proteomes can rapidly change during tissue homogenization by the action of enzymes released from their compartments, disease specific protein species patterns can vanish. Recently, we described a novel, ultrafast and soft method for cold vaporization of tissue via desorption by impulsive vibrational excitation (DIVE) using a picosecond-infrared-laser (PIRL). Given that DIVE extraction may provide improved access to the original composition of protein species in tissues, we compared the proteome composition of tissue protein homogenates after DIVE homogenization with conventional homogenizations. A higher number of intact protein species was observed in DIVE homogenates. Due to the ultrafast transfer of proteins from tissues via gas phase into frozen condensates of the aerosols, intact protein species were exposed to a lesser extent to enzymatic degradation reactions compared with conventional protein extraction. In addition, total yield of the number of proteins is higher in DIVE homogenates, because they are very homogenous and contain almost no insoluble particles, allowing direct analysis with subsequent analytical methods without the necessity of centrifugation. Enzymatic protein modifications during tissue homogenization are responsible for changes of the in-vivo protein species composition. Cold vaporization of tissues by PIRL-DIVE is comparable with taking a snapshot at the time of the laser irradiation of the dynamic changes that occur continuously under in-vivo conditions. At that time point all biomolecules are transferred into an aerosol, which is immediately frozen. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  13. Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization

    PubMed Central

    Kwiatkowski, M.; Wurlitzer, M.; Krutilin, A.; Kiani, P.; Nimer, R.; Omidi, M.; Mannaa, A.; Bussmann, T.; Bartkowiak, K.; Kruber, S.; Uschold, S.; Steffen, P.; Lübberstedt, J.; Küpker, N.; Petersen, H.; Knecht, R.; Hansen, N.O.; Zarrine-Afsar, A.; Robertson, W.D.; Miller, R.J.D.; Schlüter, H.

    2016-01-01

    Posttranslational modifications and proteolytic processing regulate almost all physiological processes. Dysregulation can potentially result in pathologic protein species causing diseases. Thus, tissue species proteomes of diseased individuals provide diagnostic information. Since the composition of tissue proteomes can rapidly change during tissue homogenization by the action of enzymes released from their compartments, disease specific protein species patterns can vanish. Recently, we described a novel, ultrafast and soft method for cold vaporization of tissue via desorption by impulsive vibrational excitation (DIVE) using a picosecond-infrared-laser (PIRL). Given that DIVE extraction may provide improved access to the original composition of protein species in tissues, we compared the proteome composition of tissue protein homogenates after DIVE homogenization with conventional homogenizations. A higher number of intact protein species was observed in DIVE homogenates. Due to the ultrafast transfer of proteins from tissues via gas phase into frozen condensates of the aerosols, intact protein species were exposed to a lesser extent to enzymatic degradation reactions compared with conventional protein extraction. In addition, total yield of the number of proteins is higher in DIVE homogenates, because they are very homogenous and contain almost no insoluble particles, allowing direct analysis with subsequent analytical methods without the necessity of centrifugation. Biological significance Enzymatic protein modifications during tissue homogenization are responsible for changes of the in-vivo protein species composition. Cold vaporization of tissues by PIRL-DIVE is comparable with taking a snapshot at the time of the laser irradiation of the dynamic changes that occur continuously under in-vivo conditions. At that time point all biomolecules are transferred into an aerosol, which is immediately frozen. PMID:26778141

  14. Assessment of oil content and fatty acid composition variability in two economically important Hibiscus species.

    PubMed

    Wang, Ming Li; Morris, Brad; Tonnis, Brandon; Davis, Jerry; Pederson, Gary A

    2012-07-04

    The Hibiscus genus encompasses more than 300 species, but kenaf (Hibiscus cannabinus L.) and roselle (Hibiscus sabdariffa L.) are the two most economically important species within the genus. Seeds from these two Hibiscus species contain a relatively high amount of oil with two unusual fatty acids: dihydrosterculic and vernolic acids. The fatty acid composition in the oil can directly affect oil quality and its utilization. However, the variability in oil content and fatty acid composition for these two species is unclear. For these two species, 329 available accessions were acquired from the USDA germplasm collection. Their oil content and fatty acid composition were determined by nuclear magnetic resonance (NMR) and gas chromatography (GC), respectively. Using NMR and GC analyses, we found that Hibiscus seeds on average contained 18% oil and seed oil was composed of six major fatty acids (each >1%) and seven minor fatty acids (each <1%). Hibiscus cannabinus seeds contained significantly higher amounts of oil (18.14%), palmitic (20.75%), oleic (28.91%), vernolic acids (VA, 4.16%), and significantly lower amounts of stearic (3.96%), linoleic (39.49%), and dihydrosterculic acids (DHSA, 1.08%) than H. sabdariffa seeds (17.35%, 18.52%, 25.16%, 3.52%, 4.31%, 44.72%, and 1.57%, respectively). For edible oils, a higher oleic/linoleic (O/L) ratio and lower level of DHSA are preferred, and for industrial oils a high level of VA is preferred. Our results indicate that seeds from H. cannabinus may be of higher quality than H. sabdariffa seeds for these reasons. Significant variability in oil content and major fatty acids was also detected within both species. The variability in oil content and fatty acid composition revealed from this study will be useful for exploring seed utilization and developing new cultivars in these Hibiscus species.

  15. Assessment of total arsenic and arsenic species stability in alga samples and their aqueous extracts.

    PubMed

    García Salgado, S; Quijano Nieto, M A; Bonilla Simón, M M

    2008-05-30

    In order to achieve reliable information on speciation analysis, it is necessary to assess previously the species stability in the sample to analyse. Furthermore, in those cases where the sample treatment for species extraction is time-consuming, an assessment of the species integrity in the extracts is of paramount importance. Thus, the present paper reports total arsenic and arsenic species stability in alga samples (Sargassum fulvellum and Hizikia fusiformis), as well as in their aqueous extracts, which were stored in amber glass and polystyrene containers at different temperatures. Total arsenic determination was carried out by inductively coupled plasma atomic emission spectroscopy (ICP-AES), after sample acid digestion in a microwave oven, while arsenic speciation was conducted by anion exchange high performance liquid chromatography on-line coupled to ICP-AES, with and without sample introduction by hydride generation (HPLC-ICP-AES and HPLC-HG-ICP-AES), after aqueous microwave-assisted extraction. The results obtained for solid alga samples showed that total arsenic (for Hijiki alga) and arsenic species present (As(V) for Hijiki and NIES No. 9 Sargasso) are stable for at least 12 months when samples are stored in polystyrene containers at +20 degrees C. On the other hand, a different behaviour was observed in the stability of total arsenic and As(V) species in aqueous extracts for both samples, being the best storage conditions for Sargasso extracts a temperature of -18 degrees C and polystyrene containers, under which they are stable for at least 15 days, while Hijiki extracts must be stored in polystyrene containers at +4 degrees C in order to ensure the stability for 10 days.

  16. Species composition and abundance of Brevipalpus spp. on different citrus species in Mexican orchards.

    PubMed

    Salinas-Vargas, D; Santillán-Galicia, M T; Valdez-Carrasco, J; Mora-Aguilera, G; Atanacio-Serrano, Y; Romero-Pescador, P

    2013-08-01

    We studied the abundance of Brevipalpus spp. in citrus orchards in the Mexican states of Yucatan, Quintana Roo and Campeche. Mites were collected from 100 trees containing a mixture of citrus species where sweet orange was always the main species. Eight collections were made at each location from February 2010 to February 2011. Mites from the genus Brevipalpus were separated from other mites surveyed and their abundance and relationships with the different citrus species were quantified throughout the collection period. A subsample of 25% of the total Brevipalpus mites collected were identified to species level and the interaction of mite species and citrus species were described. Brevipalpus spp. were present on all collection dates and their relative abundance was similar on all citrus species studies. The smallest number of mites collected was during the rainy season. Brevipalpus phoenicis (Geijskes) and Brevipalpus californicus (Banks) were the only two species present and they were found in all locations except Campeche, where only B. phoenicis was present. Yucatan and Campeche are at greater risk of leprosis virus transmission than Quintana Roo because the main vector, B. phoenicis, was more abundant than B. californicus. The implications of our results for the design of more accurate sampling and control methods for Brevipalpus spp. are discussed.

  17. Hydro-edaphic conditions defining richness and species composition in savanna areas of the northern Brazilian Amazonia.

    PubMed

    Araújo, Maria Aparecida de Moura; da Rocha, Antônio Elielson Sousa; Miranda, Izildinha de Souza; Barbosa, Reinaldo Imbrozio

    2017-01-01

    Studies on plant communities in the Amazon have reported that different hydro-edaphic conditions can affect the richness and the species composition of different ecosystems. However, this aspect is poorly known in the different savanna habitats. Understanding how populations and plant communities are distributed in these open vegetation areas is important to improve the knowledge about which environmental variables influence the occurrence and diversity of plants in this type of regional ecosystem. Thus, this study investigated the richness and composition of plant species in two savanna areas of the northern Brazilian Amazonia, using the coverage (%) of the different life forms observed under different hydro-edaphic conditions as a structural reference. We report 128 plant species classified in 34 botanical families distributed in three savanna habitats with different levels of hydro-edaphic restrictions. In this study, the habitats are conceptually presented and they integrate environmental information (edaphic factors and drainage type), which determines differences between floristic composition, species richness and coverage (%) of plant life forms.

  18. Development of an Integrated Moisture Index for predicting species composition

    Treesearch

    Louis R. Iverson; Charles T. Scott; Martin E. Dale; Anantha Prasad

    1996-01-01

    A geographic information system (GIS) approach was used to develop an Integrated Moisture Index (IMI), which was used to predict species composition for Ohio forests. Several landscape features (a slope-aspect shading index, cumulative flow of water downslope, curvature of the landscape, and the water-holding capacity of the soil) were derived from elevation and soils...

  19. Long-Term Changes in Species Composition and Relative Abundances of Sharks at a Provisioning Site

    PubMed Central

    Brunnschweiler, Juerg M.; Abrantes, Kátya G.; Barnett, Adam

    2014-01-01

    Diving with sharks, often in combination with food baiting/provisioning, has become an important product of today’s recreational dive industry. Whereas the effects baiting/provisioning has on the behaviour and abundance of individual shark species are starting to become known, there is an almost complete lack of equivalent data from multi-species shark diving sites. In this study, changes in species composition and relative abundances were determined at the Shark Reef Marine Reserve, a multi-species shark feeding site in Fiji. Using direct observation sampling methods, eight species of sharks (bull shark Carcharhinus leucas, grey reef shark Carcharhinus amblyrhynchos, whitetip reef shark Triaenodon obesus, blacktip reef shark Carcharhinus melanopterus, tawny nurse shark Nebrius ferrugineus, silvertip shark Carcharhinus albimarginatus, sicklefin lemon shark Negaprion acutidens, and tiger shark Galeocerdo cuvier) displayed inter-annual site fidelity between 2003 and 2012. Encounter rates and/or relative abundances of some species changed over time, overall resulting in more individuals (mostly C. leucas) of fewer species being encountered on average on shark feeding dives at the end of the study period. Differences in shark community composition between the years 2004–2006 and 2007–2012 were evident, mostly because N. ferrugineus, C. albimarginatus and N. acutidens were much more abundant in 2004–2006 and very rare in the period of 2007–2012. Two explanations are offered for the observed changes in relative abundances over time, namely inter-specific interactions and operator-specific feeding protocols. Both, possibly in combination, are suggested to be important determinants of species composition and encounter rates, and relative abundances at this shark provisioning site in Fiji. This study, which includes the most species from a spatially confined shark provisioning site to date, suggests that long-term provisioning may result in competitive exclusion

  20. Long-term changes in species composition and relative abundances of sharks at a provisioning site.

    PubMed

    Brunnschweiler, Juerg M; Abrantes, Kátya G; Barnett, Adam

    2014-01-01

    Diving with sharks, often in combination with food baiting/provisioning, has become an important product of today's recreational dive industry. Whereas the effects baiting/provisioning has on the behaviour and abundance of individual shark species are starting to become known, there is an almost complete lack of equivalent data from multi-species shark diving sites. In this study, changes in species composition and relative abundances were determined at the Shark Reef Marine Reserve, a multi-species shark feeding site in Fiji. Using direct observation sampling methods, eight species of sharks (bull shark Carcharhinus leucas, grey reef shark Carcharhinus amblyrhynchos, whitetip reef shark Triaenodon obesus, blacktip reef shark Carcharhinus melanopterus, tawny nurse shark Nebrius ferrugineus, silvertip shark Carcharhinus albimarginatus, sicklefin lemon shark Negaprion acutidens, and tiger shark Galeocerdo cuvier) displayed inter-annual site fidelity between 2003 and 2012. Encounter rates and/or relative abundances of some species changed over time, overall resulting in more individuals (mostly C. leucas) of fewer species being encountered on average on shark feeding dives at the end of the study period. Differences in shark community composition between the years 2004-2006 and 2007-2012 were evident, mostly because N. ferrugineus, C. albimarginatus and N. acutidens were much more abundant in 2004-2006 and very rare in the period of 2007-2012. Two explanations are offered for the observed changes in relative abundances over time, namely inter-specific interactions and operator-specific feeding protocols. Both, possibly in combination, are suggested to be important determinants of species composition and encounter rates, and relative abundances at this shark provisioning site in Fiji. This study, which includes the most species from a spatially confined shark provisioning site to date, suggests that long-term provisioning may result in competitive exclusion among shark

  1. Plant species composition alters the sign and strength of an emergent multi-predator effect by modifying predator foraging behaviour.

    PubMed

    Wilby, Andrew; Anglin, Linda Anderson; Nesbit, Christopher M

    2013-01-01

    The prediction of pest-control functioning by multi-predator communities is hindered by the non-additive nature of species functioning. Such non-additivity, commonly termed an emergent multi-predator effect, is known to be affected by elements of the ecological context, such as the structure and composition of vegetation, in addition to the traits of the predators themselves. Here we report mesocosm experiments designed to test the influence of plant density and species composition (wheat monoculture or wheat and faba bean polyculture) on the emergence of multi-predator effects between Adalia bipunctata and Chrysoperla carnea, in their suppression of populations of the aphid Metopolophium dirhodum. The mesocosm experiments were followed by a series of behavioural observations designed to identify how interactions among predators are modified by plant species composition and whether these effects are consistent with the observed influence of plant species composition on aphid population suppression. Although plant density was shown to have no influence on the multi-predator effect on aphid population growth, plant composition had a marked effect. In wheat monoculture, Adalia and Chrysoperla mixed treatments caused greater suppression of M. dirhodum populations than expected. However this positive emergent effect was reversed to a negative multi-predator effect in wheat and faba bean polyculture. The behavioural observations revealed that although dominant individuals did not respond to the presence of faba bean plants, the behaviour of sub-dominants was affected markedly, consistent with their foraging for extra-floral nectar produced by the faba bean. This interaction between plant composition and predator community composition on the foraging behaviour of sub-dominants is thought to underlie the observed effect of plant composition on the multi-predator effect. Thus, the emergence of multi-predator effects is shown to be strongly influenced by plant species

  2. Intra-plant Variation in Nectar Sugar Composition in Two Aquilegia Species (Ranunculaceae): Contrasting Patterns under Field and Glasshouse Conditions

    PubMed Central

    Canto, Azucena; Pérez, Ricardo; Medrano, Mónica; Castellanos, María Clara; Herrera, Carlos M.

    2007-01-01

    Background and Aims Intra-specific variation in nectar chemistry under natural conditions has been only rarely explored, yet it is an essential aspect of our understanding of how pollinator-mediated selection might act on nectar traits. This paper examines intra-specific variation in nectar sugar composition in field and glasshouse plants of the bumblebee-pollinated perennial herbs Aquilegia vulgaris subsp. vulgaris and Aquilegia pyrenaica subsp. cazorlensis (Ranunculaceae). The aims of the study are to assess the generality of extreme intra-plant variation in nectar sugar composition recently reported for other species in the field, and gaining insight on the possible mechanisms involved. Methods The proportions of glucose, fructose and sucrose in single-nectary nectar samples collected from field and glasshouse plants were determined using high performance liquid chromatography. A hierarchical variance partition was used to dissect total variance into components due to variation among plants, flowers within plants, and nectaries within flowers. Key Results Nectar of the two species was mostly sucrose-dominated, but composition varied widely in the field, ranging from sucrose-only to fructose-dominated. Most intra-specific variance was due to differences among nectaries of the same flower, and flowers of the same plant. The high intra-plant variation in sugar composition exhibited by field plants vanished in the glasshouse, where nectar composition emerged as a remarkably constant feature across plants, flowers and nectaries. Conclusions In addition to corroborating the results of previous studies documenting extreme intra-plant variation in nectar sugar composition in the field, this study suggests that such variation may ultimately be caused by biotic factors operating on the nectar in the field but not in the glasshouse. Pollinator visitation and pollinator-borne yeasts are suggested as likely causal agents. PMID:17259227

  3. Chemical composition of Achillea schischkinii Sosn., an endemic species from Turkey

    USDA-ARS?s Scientific Manuscript database

    In this study, we investigated the chemical composition of an endemic species of Achillea schischkinii Sosn. (Asteraceae) collected from an eastern part of Turkey (Van). Air-dried aerial parts were subjected to water-distillation using a Clevenger-type system. The essential oil was analyzed by GC-MS...

  4. A merchantable and total height model for tree species in Maine

    Treesearch

    James A. Westfall; Kenneth M. Laustsen

    2006-01-01

    A model for predicting merchantable and total tree height for 18 species groups in Maine is presented. Only tree-level predictor variables are used, so stand-level attributes, such as age and site quality, are not required. A mixed-effects modeling approach accounts for the correlated within-tree measurements. Data-collection protocols encompass situations in which...

  5. [Species composition, diversity and density of small fishes in two different habitats in Niushan Lake].

    PubMed

    Ye, Shao-Wen; Li, Zhong-Jie; Cao, Wen-Xuan

    2007-07-01

    This paper studied the spatial distribution of small fishes in a shallow macrophytic lake, Niushan Lake in spring 2003, and its relations with habitat heterogeneity. Based on the macrophyte cover condition, distance from lake shore and water depth, two representative habitat types in the lake were selected. Habitat A was near the shore with dense submersed macrophyte, while habitat B was far from the shore with sparse submersed macrophyte. Small fishes were sampled quantitatively by block net (180 m2), and their densities within the net area were estimated by multiple mark-recapture or Zippin's removal method. The results showed that there were some differences in species composition, biodiversity measurement, and estimated density of small fishes between the two habitats: 1) the catches in habitat A consisted of 14 small fish species from 5 families, among which, benthopelagic species Rhodeus ocellatus, Paracheilognathus imberbis and Pseudorasbora parva were considered as dominant species, while those in habitat B consisted of 9 small fish species from 3 families, among which, bottom species Rhinogobius giurinus and Micropercops swinhonis were dominant; 2) the Bray-Curtis index between the two small fish communities was 0.222, reflecting their low structure similarity, and no significant difference was observed between their rank/ abundance distributions, both of which belonged to log series distribution; 3) the total density of 9 major species in habitat A was 8.71 ind x m(-2), while that of 5 major species in habitat B was only 3.54 ind x m(-2). The fact that the spatial distribution of the small fishes differed with habitats might be related to their habitat need for escaping predators, feeding, and breeding, and thus, aquatic macrophyte habitat should be of significance in the rational exploitation of small fish resources as well as the conservation of fish resource diversity.

  6. Essential Oil Composition and Volatile Profile of Seven Helichrysum Species Grown in Italy.

    PubMed

    Giovanelli, Silvia; De Leo, Marinella; Cervelli, Claudio; Ruffoni, Barbara; Ciccarelli, Daniela; Pistelli, Luisa

    2018-03-06

    Helichrysum genus consists of about 600 species widespread throughout the world, especially in South Africa and in the Mediterranean area. In this study the aroma profile (HS-SPME) and the EO compositions of seven Helichrysum species (H. cymosum, H. odoratissimum, H. petiolare, H. fontanesii, H. saxatile, H. sanguineum, and H. tenax) were evaluated. All the plants were grown in Italy under the same growth conditions. The volatile constituents, particularly monoterpenes, depended by the plant's genotype and ecological adaptation. This study represents the first headspace evaluation on the selected plants and the results evidenced that monoterpenes represented the main class of constituents in five of the seven species analysed (from 59.2% to 95.0%). The higher content in sesquiterpene hydrocarbons was observed in the Mediterranean species of H. sanguineum (68.0%). Only H. saxatile showed relative similar abundance of monoterpenes and sesquiterpene hydrocarbons. The essential oil composition of the majority of examined species are characterised by high percentage of sesquiterpenes (especially β-caryophyllene and δ-cadinene) ranging from 51.3% to 92.0%, except for H. cymosum, H. tenax, and H. sanguineum leaves where monoterpenes predominated (from 51.7% to 74.7%). © 2018 Wiley-VHCA AG, Zurich, Switzerland.

  7. Main sugar composition of floral nectar in three species groups of Scrophularia (Scrophulariaceae) with different principal pollinators.

    PubMed

    Rodríguez-Riaño, T; Ortega-Olivencia, A; López, J; Pérez-Bote, J L; Navarro-Pérez, M L

    2014-11-01

    In some angiosperm groups, a parallelism between nectar traits and pollination syndromes has been demonstrated, whereas in others there is not such relationship and it has been explained as due to phylogenetic constraints. However, nectar trait information remains scarce for many plant groups. This paper focuses on three groups of Scrophularia species, with different flower sizes and principal pollinators, to find out whether nectar sugar composition is determined by pollinator type or reflects taxonomic affinities. Since the species we examined have protogynous flowers, and gender bias in nectar sugar composition has been noted in few plant groups, we also investigated whether sexual phase influenced Scrophularia nectar composition. The sugar composition was found to be similar in all species, having high-sucrose nectar, except for the Macaronesian Scrophularia calliantha, which was the only species with balanced nectar; this last kind of nectar could be associated with the high interaction rates observed between S. calliantha and passerine birds. The nectar sugar composition (high in sucrose) was unrelated to the principal pollinator group, and could instead be considered a conservative taxonomic trait. No gender bias was observed between functionally female and male flowers for nectar volume or concentration. However, sexual phase significantly affected sucrose percentage in the largest-flowered species, where the female phase flowers had higher sucrose percentages than the male phase flowers. © 2014 German Botanical Society and The Royal Botanical Society of the Netherlands.

  8. Retention of Anionic Species on Granite: Influence of Granite Composition - 12129

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Videnska, Katerina; Havlova, Vaclava

    Technetium (Tc-99, T{sub 1/2} = 2.1.10{sup 5} yrs) and selenium (Se-79, T{sub 1/2} = 6.5.10{sup 4} yrs) belong among fission products, being produced by fission of nuclear fuel. Both elements can significantly contribute to risk due to their complicated chemistry, long life times, high mobility and prevailing anionic character. Therefore, knowledge of migration behaviour under different conditions can significantly improve input into performance and safety assessment models. Granite is considered as a potential host rock for deep geological disposal of radioactive waste in many countries. Granitic rocks consist usually of quartz, feldspar, plagioclase (main components), mica, chlorite, kaolinite (minor components).more » The main feature of the rock is advection governed transport in fractures, complemented with diffusion process from fracture towards undisturbed rock matrix. The presented work is focused on interaction of anionic species (TcO{sub 4}{sup -}, SeO{sub 4}{sup 2-}, SeO{sub 3}{sup 2-}) with granitic rock. Furthermore, the importance of mineral composition on sorption of anionic species was also studied. The batch sorption experiments were conducted on the crushed granite from Bohemian Massive. Five fractions with defined grain size were used for static batch method. Mineral composition of each granitic fraction was evaluated using X-ray diffraction. The results showed differences in composition of granitic fractions, even though originating from one homogenized material. Sorption experiments showed influence of granite composition on adsorption of both TcO4{sup -} and SeO3{sup 2-} on granitic rock. Generally, Se(IV) showed higher retention than Tc(VII). Se(VI) was not almost sorbed at all. Fe containing minerals are pronounced as a selective Se and Tc sorbent, being reduced on their surface. As micas in granite are usually enriched in Fe, increased sorption of anionic species onto mica enriched fractions can be explained by this reason. On the other

  9. Species Richness Responses to Structural or Compositional Habitat Diversity between and within Grassland Patches: A Multi-Taxon Approach

    PubMed Central

    Lengyel, Szabolcs; Déri, Eszter; Magura, Tibor

    2016-01-01

    Habitat diversity (spatial heterogeneity within and between habitat patches in a landscape, HD) is often invoked as a driver of species diversity at small spatial scales. However, the effect of HD on species richness (SR) of multiple taxa is not well understood. We quantified HD and SR in a wet-dry gradient of open grassland habitats in Hortobágy National Park (E-Hungary) and tested the effect of compositional and structural factors of HD on SR of flowering plants, orthopterans, true bugs, spiders, ground beetles and birds. Our dataset on 434 grassland species (170 plants, 264 animals) showed that the wet-dry gradient (compositional HD at the between-patch scale) was primarily related to SR in orthopterans, ground-dwelling arthropods, and all animals combined. The patchiness, or plant association richness, of the vegetation (compositional HD at the within-patch scale) was related to SR of vegetation-dwelling arthropods, whereas vegetation height (structural HD at the within-patch scale) was related to SR of ground-dwelling arthropods and birds. Patch area was related to SR only in birds, whereas management (grazing, mowing, none) was related to SR of plants and true bugs. All relationships between HD and SR were positive, indicating increasing SR with increasing HD. However, total SR was not related to HD because different taxa showed similar positive responses to different HD variables. Our findings, therefore, show that even though HD positively influences SR in a wide range of grassland taxa, each taxon responds to different compositional or structural measures of HD, resulting in the lack of a consistent relationship between HD and SR when taxon responses are pooled. The idiosyncratic responses shown here exemplify the difficulties in detecting general HD-SR relationships over multiple taxa. Our results also suggest that management and restoration aimed specifically to sustain or increase the diversity of habitats are required to conserve biodiversity in

  10. Chemical composition and antioxidant activity of certain Morus species

    PubMed Central

    Imran, Mohammad; Khan, Hamayun; Shah, Mohibullah; Khan, Rasool; Khan, Faridullah

    2010-01-01

    In the present work, the fruits of four Morus species, namely Morus alba (white mulberry), Morus nigra (black mulberry), Morus laevigata (large white fruit), and Morus laevigata (large black fruit), were analyzed for proximate composition, essential minerals, and antioxidant potentials. For this purpose, the ripe fruits were collected from the northern regions of Pakistan. The major nutritional components (moisture, ash, lipids, proteins, fibres, carbohydrates, and total sugar) were found to be in the suitable range along with good computed energy. Total dry weight, pH, and titratable acidity (percent citric acid) were (17.60±1.94)–(21.97±2.34) mg/100 g, (3.20±0.07)–(4.78±0.15), and (0.84±0.40)%–(2.00±0.08)%, respectively. Low riboflavin (vitamin B2) and niacin (vitamin B3) contents were recorded in all the fruits, while ascorbic acid (vitamin C) was in the range from (15.20±1.25) to (17.03±1.71) mg/100 g fresh weight (FW). The mulberry fruits were rich with regard to the total phenol and alkaloid contents, having values of (880±7.20)–(1650±12.25) mg/100 g FW and (390±.22)–(660±5.25) mg/100 g FW, respectively. Sufficient quantities of essential macro-(K, Ca, Mg, and Na) and micro-(Fe, Zn, and Ni) elements were found in all the fruits. K was the predominant element with concentration ranging from (1270±9.36) to (1731±11.50) mg/100 g, while Ca, Na, and Mg contents were (440±3.21)–(576±7.37), (260±3.86)–(280±3.50), and (24±3.51)–(360±4.20) mg/100 g, respectivly. The decreasing order of micro-minerals was Fe>Zn>Ni. The radical scavenging activity of methanolic extract of fruits was concentration-dependent and showed a correlation with total phenolic constituents of the respective fruits. Based on the results obtained, mulberry fruits were found to serve as a potential source of food diet and natural antioxidants. PMID:21121077

  11. Sandflies species composition, activity, and natural infection with Leishmania, parasite identity in lesion isolates of cutaneous leishmaniasis, central Iran.

    PubMed

    Gholamian-Shahabad, Mahmood Reza; Azizi, Kourosh; Asgari, Qasem; Kalantari, Mohsen; Moemenbellah-Fard, Mohammad Djaefar

    2018-06-01

    Zoonotic cutaneous leishmaniosis (ZCL) is a crucial public health challenge in Iran. Sandflies feed on reservoir rodents' blood infected with Leishmania parasite and transmit it to other hosts. This study was conducted to find out the composition and monthly activity of sandflies as well as to identify the protozoan pathogens ( Leishmania / Crithidia ) by polymerase chain reaction (PCR) in an emerging ZCL focus of Abarkooh, Yazd province, Iran, in 2016. A cross-sectional study was done in rural areas of Abarkooh. From April to November 2016, sticky traps were used indoor and outdoor to capture sandflies once every fortnight. Their composition and monthly activity were recorded. Following identification of sandflies and DNA extraction from them, PCR was used to identify their parasite and match it against samples taken from ZCL confirmed and suspected patients' lesions. After collection, a total of 2045 sandflies (779 indoor, 1266 outdoor) were identified to species level. Sandfly activity started early April in this area with two active peaks (one late May and the other late August) terminated about mid-November. Seven Phlebotomus species and three Sergentomyia species were identified. The most and the least abundant species were P. papatasi (40.1%) and P. alexandri (0.09%), respectively. Using PCR, only 6% (12:200) of P. papatasi sandflies were infected with Leishmania parasite. No Crithidia was detected in either sandflies or human lesions (176 specimen). Based on the highest abundance both indoor and outdoor of P. papatasi , this sandfly was considered the main vector of ZCL in this area. The capture of P. caucasicus , P. mongolensis , and P. ansarii from rodent burrows showed these species were likely involved in pathogen transmission in reservoir rodents' burrows.

  12. Tree species composition affects the abundance of rowan (Sorbus aucuparia L.) in urban forests in Finland.

    PubMed

    Hamberg, Leena; Lehvävirta, Susanna; Kotze, D Johan; Heikkinen, Juha

    2015-03-15

    Recent studies have shown a considerable increase in the abundance of rowan (Sorbus aucuparia) saplings in urban forests in Finland, yet the reasons for this increase are not well understood. Here we investigated whether canopy cover or tree species composition, i.e., the basal areas of different tree species in Norway spruce dominated urban forests, affects the abundances of rowan seedlings, saplings and trees. Altogether 24 urban forest patches were investigated. We sampled the number of rowan and other saplings, and calculated the basal areas of trees. We showed that rowan abundance was affected by tree species composition. The basal area of rowan trees (≥ 5 cm in diameter at breast height, dbh) decreased with increasing basal area of Norway spruce, while the cover of rowan seedlings increased with an increase in Norway spruce basal area. However, a decrease in the abundance of birch (Betula pendula) and an increase in the broad-leaved tree group (Acer platanoides, Alnus glutinosa, Alnus incana, Amelanchier spicata, Prunus padus, Quercus robur, Rhamnus frangula and Salix caprea) coincided with a decreasing number of rowans. Furthermore, rowan saplings were scarce in the vicinity of mature rowan trees. Although it seems that tree species composition has an effect on rowan, the relationship between rowan saplings and mature trees is complex, and therefore we conclude that regulating tree species composition is not an easy way to keep rowan thickets under control in urban forests in Finland. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Impact of competitor species composition on predicting diameter growth and survival rates of Douglas-fir trees in southwestern Oregon

    USGS Publications Warehouse

    Bravo, Felipe; Hann, D.W.; Maguire, Douglas A.

    2001-01-01

    Mixed conifer and hardwood stands in southwestern Oregon were studied to explore the hypothesis that competition effects on individual-tree growth and survival will differ according to the species comprising the competition measure. Likewise, it was hypothesized that competition measures should extrapolate best if crown-based surrogates are given preference over diameter-based (basal area based) surrogates. Diameter growth and probability of survival were modeled for individual Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) trees growing in pure stands. Alternative models expressing one-sided and two-sided competition as a function of either basal area or crown structure were then applied to other plots in which Douglas-fir was mixed with other conifers and (or) hardwood species. Crown-based variables outperformed basal area based variables as surrogates for one-sided competition in both diameter growth and survival probability, regardless of species composition. In contrast, two-sided competition was best represented by total basal area of competing trees. Surrogates reflecting differences in crown morphology among species relate more closely to the mechanics of competition for light and, hence, facilitate extrapolation to species combinations for which no observations are available.

  14. A Review of the Composition of the Essential Oils and Biological Activities of Angelica Species.

    PubMed

    Sowndhararajan, Kandasamy; Deepa, Ponnuvel; Kim, Minju; Park, Se Jin; Kim, Songmun

    2017-09-20

    A number of Angelica species have been used in traditional systems of medicine to treat many ailments. Especially, essential oils (EOs) from the Angelica species have been used for the treatment of various health problems, including malaria, gynecological diseases, fever, anemia, and arthritis. EOs are complex mixtures of low molecular weight compounds, especially terpenoids and their oxygenated compounds. These components deliver specific fragrance and biological properties to essential oils. In this review, we summarized the chemical composition and biological activities of EOs from different species of Angelica . For this purpose, a literature search was carried out to obtain information about the EOs of Angelica species and their bioactivities from electronic databases such as PubMed, Science Direct, Wiley, Springer, ACS, Google, and other journal publications. There has been a lot of variation in the EO composition among different Angelica species. EOs from Angelica species were reported for different kinds of biological activities, such as antioxidant, anti-inflammatory, antimicrobial, immunotoxic, and insecticidal activities. The present review is an attempt to consolidate the available data for different Angelica species on the basis of major constituents in the EOs and their biological activities.

  15. Bud Composition, Branching Patterns and Leaf Phenology in Cerrado Woody Species

    PubMed Central

    DAMASCOS, M. A.; PRADO, C. H. B. A.; RONQUIM, C. C.

    2005-01-01

    • Background and Aims Plants have complex mechanisms of aerial biomass exposition, which depend on bud composition, the period of the year in which shoot extension occurs, branching pattern, foliage persistence, herbivory and environmental conditions. • Methods The influence of water availability and temperature on shoot growth, the bud composition, the leaf phenology, and the relationship between partial leaf fall and branching were evaluated over 3 years in Cerrado woody species Bauhinia rufa (BR), Leandra lacunosa (LL) and Miconia albicans (MA). • Key Results Deciduous BR preformed organs in buds and leaves flush synchronously at the transition from the dry to the wet season. The expansion time of leaves is <1 month. Main shoots (first-order axis, A1 shoots) extended over 30 d and they did not branch. BR budding and foliage unfolds were brought about independently of inter-annual rainfall variations. By contrast, in LL and MA evergreen species, the shoot extension rate and the neoformation of aerial organs depended on rainfall. Leaf emergence was continuous for 2–6 months and lamina expansion took place over 1–4 months. The leaf life span was 5–20 months and the main A1 shoot extension happened over 122–177 d. Both evergreen species allocated biomass to shoots, leaves or flowers continuously during the year, branching in the middle of the wet season to form second-order (A2 shoots) and third-order (A3 shoots) axis in LL and A2 shoots in MA. Partial shed of A1 shoot leaves would facilitate a higher branching intensity A2 shoot production in LL than in MA. MA presented a longer leaf life span, produced a lower percentage of A2 shoots but had a higher meristem persistence on A1 and A2 shoots than LL. • Conclusions It was possible to identify different patterns of aerial growth in Cerrado woody species defined by shoot-linked traits such as branching pattern, bud composition, meristem persistence and leaf phenology. These related traits must be

  16. Divergent composition but similar function of soil food webs of individual plants: plant species and community effects.

    PubMed

    Bezemer, T M; Fountain, M T; Barea, J M; Christensen, S; Dekker, S C; Duyts, H; van Hal, R; Harvey, J A; Hedlund, K; Maraun, M; Mikola, J; Mladenov, A G; Robin, C; de Ruiter, P C; Scheu, S; Setälä, H; Smilauer, P; van der Putten, W H

    2010-10-01

    Soils are extremely rich in biodiversity, and soil organisms play pivotal roles in supporting terrestrial life, but the role that individual plants and plant communities play in influencing the diversity and functioning of soil food webs remains highly debated. Plants, as primary producers and providers of resources to the soil food web, are of vital importance for the composition, structure, and functioning of soil communities. However, whether natural soil food webs that are completely open to immigration and emigration differ underneath individual plants remains unknown. In a biodiversity restoration experiment we first compared the soil nematode communities of 228 individual plants belonging to eight herbaceous species. We included grass, leguminous, and non-leguminous species. Each individual plant grew intermingled with other species, but all plant species had a different nematode community. Moreover, nematode communities were more similar when plant individuals were growing in the same as compared to different plant communities, and these effects were most apparent for the groups of bacterivorous, carnivorous, and omnivorous nematodes. Subsequently, we analyzed the composition, structure, and functioning of the complete soil food webs of 58 individual plants, belonging to two of the plant species, Lotus corniculatus (Fabaceae) and Plantago lanceolata (Plantaginaceae). We isolated and identified more than 150 taxa/groups of soil organisms. The soil community composition and structure of the entire food webs were influenced both by the species identity of the plant individual and the surrounding plant community. Unexpectedly, plant identity had the strongest effects on decomposing soil organisms, widely believed to be generalist feeders. In contrast, quantitative food web modeling showed that the composition of the plant community influenced nitrogen mineralization under individual plants, but that plant species identity did not affect nitrogen or carbon

  17. Seasonal variations in biomass and species composition of seaweeds along the northern coasts of Persian Gulf (Bushehr Province)

    NASA Astrophysics Data System (ADS)

    Dadolahi-Sohrab, A.; Garavand-Karimi, M.; Riahi, H.; Pashazanoosi, H.

    2012-02-01

    This study was carried out to evaluate the seasonal variations of seaweed biomass and species composition at six different sites along the coastal areas in Bushehr Province. Sampling depths varied among sites, from 0.3 to 2.0 m below mean sea level. A total of 37 (i.e., 10 Chlorophyta, 12 Phaeophyta and 15 Rhodophyta) seaweed species were collected. Studies were conducted for quantifying the seaweeds during four seasons from October 2008 until July 2009. During present research, Ulva intestinalis and Cladophora nitellopsis of green, Polycladia myrica, Sirophysalia trinodis and Sargassum angustifolium of brown and Gracilaria canaliculata and Hypnea cervicornis of red seaweeds showed highest biomass in coastal areas of Bushehr Province. The Cheney`s ratio of 2.1 indicated a temperate algal flora to this area. All sites exhibited more than 50% similarity of algal species, indicating a relatively homogenous algal distribution. Total biomass showed the highest value of 3280.7 ± 537.8 g dry wt m - 2 during summer and lowest value of 856.9 ± 92.0 g dry wt m - 2 during winter. During this study, the highest and lowest seaweed biomass were recorded on the site 2 (2473.7 ± 311.0 g dry wt m - 2) and site 5 (856.7 ± 96.8 g dry wt m - 2), respectively.

  18. Hydro-edaphic conditions defining richness and species composition in savanna areas of the northern Brazilian Amazonia

    PubMed Central

    Araújo, Maria Aparecida de Moura; da Rocha, Antônio Elielson Sousa; Miranda, Izildinha de Souza

    2017-01-01

    Abstract Background Studies on plant communities in the Amazon have reported that different hydro-edaphic conditions can affect the richness and the species composition of different ecosystems. However, this aspect is poorly known in the different savanna habitats. Understanding how populations and plant communities are distributed in these open vegetation areas is important to improve the knowledge about which environmental variables influence the occurrence and diversity of plants in this type of regional ecosystem. Thus, this study investigated the richness and composition of plant species in two savanna areas of the northern Brazilian Amazonia, using the coverage (%) of the different life forms observed under different hydro-edaphic conditions as a structural reference. New information We report 128 plant species classified in 34 botanical families distributed in three savanna habitats with different levels of hydro-edaphic restrictions. In this study, the habitats are conceptually presented and they integrate environmental information (edaphic factors and drainage type), which determines differences between floristic composition, species richness and coverage (%) of plant life forms. PMID:28848372

  19. Zooplankton Distribution and Species Composition Along an Oxygen Gradient in Puget Sound, WA

    NASA Astrophysics Data System (ADS)

    Keister, J. E.; Essington, T.; Li, L.; Horne, J. K.; Sato, M.; Parker-Stetter, S. L.; Moriarty, P.

    2016-02-01

    Low dissolved oxygen (hypoxia) is one of the most pronounced, pervasive, and significant disturbances in marine ecosystems, yet our understanding of its effects is incomplete, particularly with respect to impacts on lower trophic levels. As part of a study of how hypoxia affects predator-prey relationships and energy flow through marine food webs, we are studying relationships between ocean chemistry and zooplankton in Puget Sound, Washington—a deep, seasonally hypoxic fjord in the Pacific Northwest that supports a productive and diverse pelagic community. From summer through fall in two years that differed in the timing and intensity of hypoxia, we conducted multi-frequency bioacoustic surveys, CTD casts, and depth-stratified zooplankton sampling to examine changes in distribution and species composition of animals in relation to oxygen concentrations. We exploited a natural gradient in oxygen along the axis of the fjord by sampling at moderately hypoxic and normoxic sites with otherwise similar hydrography and species composition to disentangle the effects of oxygen from changes in other environmental factors. Our results support the hypothesis that zooplankton species composition and vertical distributions are altered by hypoxia, but only when examined at the species and life-stage level. Relatively few taxa showed clear responses to hypoxia, and bioacoustic backscatter data (which was dominated by adult euphausiids and amphipods) indicated that those taxa were not affected by the levels of hypoxia we observed. Examination of net tow data revealed more subtle changes, including behavioral avoidance of low oxygen by some copepods and young euphausiid life stages. Overall, the high species diversity and relatively low susceptibility of many zooplankton to hypoxia in Puget Sound may confer ecosystem resilience to near-future projected changes in this region.

  20. Grazing effects on species composition in different vegetation types (La Palma, Canary Islands)

    NASA Astrophysics Data System (ADS)

    Arévalo, J. R.; de Nascimento, L.; Fernández-Lugo, S.; Mata, J.; Bermejo, L.

    2011-05-01

    Grazing management is probably one of the most extensive land uses, but its effects on plant communities have in many cases been revealed to be contradictory. Some authors have related these contradictions to the stochastic character of grazing systems. Because of that, it is necessary to implement specific analyses of grazing effects on each community, especially in natural protected areas, in order to provide the best information to managers. We studied the effects of grazing on the species composition of the main vegetation types where it takes place (grasslands, shrublands and pine forests) on the island of La Palma, Canary Islands. We used the point-quadrat intersect method to study the species composition of grazed and ungrazed areas, which also were characterized by their altitude, distance to farms, distance to settlements, year of sampling, herbaceous aboveground biomass and soil organic matter. The variables organic matter, productivity and species richness were not significantly affected by grazing. The species composition of the analyzed plant communities was affected more by variables such as altitude or distance to farms than by extensive grazing that has been traditionally carried out on the island of La Palma involving certain practices such as continuous monitoring of animals by goat keepers, medium stocking rates adjusted to the availability of natural pastures, supplementation during the dry season using local forage shrubs or mown pastures and rotating animals within grazing areas Although some studies have shown a negative effect of grazing on endangered plant species, these results cannot be freely extrapolated to the traditional grazing systems that exert a low pressure on plant communities (as has been found in this study). We consider extensive grazing as a viable way of ensuring sustainable management of the studied ecosystems.

  1. Vegetation in Bangalore's Slums: Composition, Species Distribution, Density, Diversity, and History

    NASA Astrophysics Data System (ADS)

    Gopal, Divya; Nagendra, Harini; Manthey, Michael

    2015-06-01

    There is widespread acknowledgement of the need for biodiversity and greening to be part of urban sustainability efforts. Yet we know little about greenery in the context of urban poverty, particularly in slums, which constitute a significant challenge for inclusive development in many rapidly growing cities. We assessed the composition, density, diversity, and species distribution of vegetation in 44 slums of Bangalore, India, comparing these to published studies on vegetation diversity in other land-use categories. Most trees were native to the region, as compared to other land-use categories such as parks and streets which are dominated by introduced species. Of the most frequently encountered tree species, Moringa oleifera and Cocos nucifera are important for food, while Ficus religiosa plays a critical cultural and religious role. Tree density and diversity were much lower in slums compared to richer residential neighborhoods. There are also differences in species preferences, with most plant (herb, shrub and vines) species in slums having economic, food, medicinal, or cultural use, while the species planted in richer residential areas are largely ornamental. Historic development has had an impact on species distribution, with older slums having larger sized tree species, while recent slums were dominated by smaller sized tree species with greater economic and food use. Extensive focus on planting trees and plant species with utility value is required in these congested neighborhoods, to provide livelihood support.

  2. Assemblage composition of fungal wood-decay species has a major influence on how climate and wood quality modify decomposition.

    PubMed

    Venugopal, Parvathy; Junninen, Kaisa; Edman, Mattias; Kouki, Jari

    2017-03-01

    The interactions among saprotrophic fungal species, as well as their interactions with environmental factors, may have a major influence on wood decay and carbon release in ecosystems. We studied the effect that decomposer diversity (species richness and assemblage composition) has on wood decomposition when the climatic variables and substrate quality vary simultaneously. We used two temperatures (16 and 21°C) and two humidity levels (70% and 90%) with two wood qualities (wood from managed and old-growth forests) of Pinus sylvestris. In a 9-month experiment, the effects of fungal diversity were tested using four wood-decaying fungi (Antrodia xantha, Dichomitus squalens, Fomitopsis pinicola and Gloeophyllum protractum) at assemblage levels of one, two and four species. Wood quality and assemblage composition affected the influence of climatic factors on decomposition rates. Fungal assemblage composition was found to be more important than fungal species richness, indicating that species-specific fungal traits are of paramount importance in driving decomposition. We conclude that models containing fungal wood-decay species (and wood-based carbon) need to take into account species-specific and assemblage composition-specific properties to improve predictive capacity in regard to decomposition-related carbon dynamics. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  3. Changes in Seagrass Species Composition in Northwestern Gulf of Mexico Estuaries: Effects on Associated Seagrass Fauna

    PubMed Central

    Ray, Brandon R.; Johnson, Matthew W.; Cammarata, Kirk; Smee, Delbert L.

    2014-01-01

    The objective of this study was to measure the communities associated with different seagrass species to predict how shifts in seagrass species composition may affect associated fauna. In the northwestern Gulf of Mexico, coverage of the historically dominant shoal grass (Halodule wrightii) is decreasing, while coverage of manatee grass (Syringodium filiforme) and turtle grass (Thalassia testudinum) is increasing. We conducted a survey of fishes, crabs, and shrimp in monospecific beds of shoal, manatee, and turtle grass habitats of South Texas, USA to assess how changes in sea grass species composition would affect associated fauna. We measured seagrass parameters including shoot density, above ground biomass, epiphyte type, and epiphyte abundance to investigate relationships between faunal abundance and these seagrass parameters. We observed significant differences in communities among three seagrass species, even though these organisms are highly motile and could easily travel among the different seagrasses. Results showed species specific relationships among several different characteristics of the seagrass community and individual species abundance. More work is needed to discern the drivers of the complex relationships between individual seagrass species and their associated fauna. PMID:25229897

  4. New way of body composition analysis using total body electrical conductivity method

    NASA Astrophysics Data System (ADS)

    Piasecki, Wojciech; Koteja, Pawel; Weiner, January; Froncisz, Wojciech

    1995-04-01

    Traditional methods of measuring total body water and fat content of animals that require sacrificing specimens are generally unacceptable when endangered species, or large animal sizes, or humans are involved. These methods are also unsuitable for following changes of fat and water content in individuals. An alternative method, based on the nonresonant absorption of a rf electromagnetic field has been used for constructing a new body composition analyzer. As the electrical conductivity of lipids is approximately 20 times lower than that of lean tissues, the rf power absorbed by the animal provides information which enables one to calculate the lean body mass and total body water. The new instrument measures rf power absorbed by an animal by measuring the quality factor (Q) of the resonant circuit with an animal placed inside the coil. Numerical calculations of the rf power absorbed by a cylindrical object containing 0.9% NaCl aqueous solution have also been performed. Experimental values confirmed the calculated dependence of the absorbed power on the cylinder radius. The device built has been calibrated on 9 males and 11 females of laboratory mice. The amount of lipids was then measured by ether extraction. The relation between instrument reading, which is proportional to the power absorption, and lean body mass (LBM) or water mass (WM) was linear and highly significant: the simple regression coefficients of determination were 0.983 for LBM, and 0.990 for WM (p<0.001). It has been found that for an individual animal with a body mass ranging from 15.9 to 40.7 g, the accuracy of measurement was ±1.6 g for LBM and ±1 g for WM.

  5. Site and plant species are important determinants of the Methylobacterium community composition in the plant phyllosphere.

    PubMed

    Knief, Claudia; Ramette, Alban; Frances, Lisa; Alonso-Blanco, Carlos; Vorholt, Julia A

    2010-06-01

    The plant phyllosphere constitutes a habitat for numerous microorganisms; among them are members of the genus Methylobacterium. Owing to the ubiquitous occurrence of methylobacteria on plant leaves, they represent a suitable target for studying plant colonization patterns. The influence of the factor site, host plant species, time and the presence of other phyllosphere bacteria on Methylobacterium community composition and population size were evaluated in this study. Leaf samples were collected from Arabidopsis thaliana or Medicago truncatula plants and from the surrounding plant species at several sites. The abundance of cultivable Methylobacterium clearly correlated with the abundance of other phyllosphere bacteria, suggesting that methylobacteria constitute a considerable and rather stable fraction of the phyllosphere microbiota under varying environmental conditions. Automated ribosomal intergenic spacer analysis (ARISA) was applied to characterize the Methylobacterium community composition and showed the presence of similar communities on A. thaliana plants at most sites in 2 consecutive years of sampling. A substantial part of the observed variation in the community composition was explained by site and plant species, especially in the case of the plants collected at the Arabidopsis sites (50%). The dominating ARISA peaks that were detected on A. thaliana plants were found on other plant species grown at the same site, whereas some different peaks were detected on A. thaliana plants from other sites. This indicates that site-specific factors had a stronger impact on the Methylobacterium community composition than did plant-specific factors and that the Methylobacterium-plant association is not highly host plant species specific.

  6. Tree species related functional properties of dissolved and total organic matter in throughfall, stemflow and forest floor solutions

    NASA Astrophysics Data System (ADS)

    Michalzik, Beate; Bischoff, Sebastian; Schwarz, Martin; Siemens, Jan; Thieme, Lisa; Wilcke, Wolfgang

    2016-04-01

    The amount and chemical nature of water-bound organic matter is a prerequisite for advancing our understanding of the C and nutrient cycling and associated ecosystem processes. While many investigations have addressed the nature and dynamics of DOM in terrestrial ecosystems, only a few have investigated the dynamics and composition of water-bound total OM (TOM) including the particulate organic matter fraction (POM; 0.45 μm < POM < 500 μm). Since water-bound element and nutrient concentrations are conventionally measured after 0.45 μm-filtration, the exclusion of the POM fraction results in misleading inferences and budgeting gaps of nutrient and energy fluxes in terrestrial ecosystems. Furthermore, tree species differ in leaf composition (e.g. nutrient, polyphenols content) and leaf litter quality, which in turn affect a variety of ecosystem processes. Nevertheless, the composition and amount of DOM and TOM derived from living plant material via throughfall (TF), stemflow (SF) and its compositional fate traversing the forest floor (FF) are insufficiently understood. In particular we asked: How do tree species and forest types affect the amount of dissolved and particulate C and N in TF and FF solutions and thus the input into the mineral soil? Do functional properties (e.g. aromaticity) of DOM and TOM differ in TF, SF and FF solutions collected in beech and spruce stands and among different beech stands across Germany? To monitor (mineral) soil input fluxes of DOM and POM in different spruce and beech forests, we fortnightly sampled TF and FF solution over three years (2010-2012) in the "Hainich-Dün-Exploratory", Thuringia, Central Germany, which forms part of the DFG SPP 1374 "Exploratories for Large-scale and Long-term Functional Biodiversity Research". To characterize chemical properties of DOM and TOM, we applied solid-state 13C NMR spectroscopy to TF, SF and FF solutions from three European beech regions across Germany and from Norway spruce sites of the

  7. pCO2 effects on species composition and growth of an ...

    EPA Pesticide Factsheets

    The effects of ongoing changes in ocean carbonate chemistry on plankton ecology have important implications for food webs and biogeochemical cycling. However, conflicting results have emerged regarding species-specific responses to pCO2 enrichment and thus community responses have been difficult to predict. To assess community level effects (e.g., production) of altered carbonate chemistry, studies are needed that capitalize on the benefits of controlled experiments but also retain features of intact ecosystems that may exacerbate or ameliorate the effects observed in single-species or single cohort experiments. We performed incubations of natural plankton communities from Narragansett Bay, RI, USA in winter at ambient bay temperatures (5–13 °C), light and nutrient concentrations under three levels of controlled and constant CO2 concentrations, simulating past, present and future conditions at mean pCO2 levels of 224, 361, and 724 μatm respectively. Samples for carbonate analysis, chlorophyll a, plankton size-abundance, and plankton species composition were collected daily and phytoplankton growth rates in three different size fractions (20 μm) were measured at the end of the 7-day incubation period. Community composition changed during the incubation period with major increases in relative diatom abundance, which were similar across pCO2 treatments. At the end of the experiment, 24-hr growth responses to pCO2 levels varied as a function of cell size. The s

  8. Species composition, diversity and structure of novel forests of Castilla elastica in Puerto Rico

    Treesearch

    J. Fonseca da Silva; NO-VALUE

    2014-01-01

    Attributes of novel forests (secondary forests containing introduced species) were compared with those of native secondary forests of similar age. The study area was the biological reserve called El Tallonal, in Puerto Rico. Species composition, tree density, basal area and soil bulk density were characterized; Importance value index (IVI), Shannon’s diversity index (H...

  9. Contrasting outcomes of species- and community-level analyses of the temporal consistency of functional composition.

    PubMed

    Katabuchi, Masatoshi; Wright, S Joseph; Swenson, Nathan G; Feeley, Kenneth J; Condit, Richard; Hubbell, Stephen P; Davies, Stuart J

    2017-09-01

    Multiple anthropogenic drivers affect every natural community, and there is broad interest in using functional traits to understand and predict the consequences for future biodiversity. There is, however, no consensus regarding the choice of analytical methods. We contrast species- and community-level analyses of change in the functional composition for four traits related to drought tolerance using three decades of repeat censuses of trees in the 50-ha Forest Dynamics Plot on Barro Colorado Island, Panama. Community trait distributions shifted significantly through time, which may indicate a shift toward more drought tolerant species. However, at the species level, changes in abundance were unrelated to trait values. To reconcile these seemingly contrasting results, we evaluated species-specific contributions to the directional shifts observed at the community level. Abundance changes of just one to six of 312 species were responsible for the community-level shifts observed for each trait. Our results demonstrate that directional changes in community-level functional composition can result from idiosyncratic change in a few species rather than widespread community-wide changes associated with functional traits. Future analyses of directional change in natural communities should combine community-, species-, and possibly individual-level analyses to uncover relationships with function that can improve understanding and enable prediction. © 2017 by the Ecological Society of America.

  10. Total lipid and fatty acid composition of eight strains of marine diatoms

    NASA Astrophysics Data System (ADS)

    Liang, Ying; Mai, Kang-Sen; Sun, Shi-Chun

    2000-12-01

    Fatty acid composition and total lipid content of 8 strains of marine diatoms ( Nitzschia frustrula, Nitzschia closterium, Nitzschia incerta, Navicula pelliculosa, Phaeodactylum tricornutum, Synedra fragilaroides) were examined. The microalgae were grown under defined conditions and harvested at the late exponential phase. The major fatty acids in most strains were 14∶0 (1.0% 6.3%), 16∶0 (13.5 26.4%), 16∶1n-7 (21.1% 46.3%) and 20∶5n-3 (6.5% 19.5%). The polyunsaturated fatty acids 16∶2n-4, 16∶3n-4, 16∶4n-1 and 20∶4n-6 also comprised a significant proportion of the total fatty acids in some strains. The characteristic fatty acid composition of diatoms is readily distinguishable from those of other microalgal groups. Significant concentration of the polyunsaturated fatty acid 20∶5n-3 (eicosapentaenoic acid) was present in each strain, with the highest proportion in B222 (19.5%).

  11. Predicting relative species composition within mixed conifer forest pixels using zero‐inflated models and Landsat imagery

    Treesearch

    Shannon L. Savage; Rick L. Lawrence; John R. Squires

    2015-01-01

    Ecological and land management applications would often benefit from maps of relative canopy cover of each species present within a pixel, instead of traditional remote-sensing based maps of either dominant species or percent canopy cover without regard to species composition. Widely used statistical models for remote sensing, such as randomForest (RF),...

  12. [Dietary composition and food competition of six main fish species in rocky reef habitat off Gouqi Island].

    PubMed

    Wang, Kai; Zhang, Shou-Yu; Wang, Zhen-Hua; Zhao, Jing; Xu, Min; Lin, Jun

    2012-02-01

    Based on the monthly investigation data of fish resources in the rocky reef habitat off Gouqi Island from March 2009 to February 2010, this paper studied the dietary composition of three native fish species (Sebasticus marmoratus, Hexagrammos otakii and Hexagrammos agrammus) and three non-native fish species (Lateolabrax japonica, Nibea albiflora and Larimichthys polyactis). The analysis of gut content indicated that the main prey items of these six dominant fish species were Caprellidae, Gammaridea, juvenile S. marmoratus, Engraulis japonicas and Acetes chinensis and the dietary composition of each of the 6 fish species had obvious seasonal variation. There was an intense food competition between native species H. otakii and H. agrammus in autumn, between non-native species N. albiflora and L. polyactis in summer, between non-native species N. albiflora and native species S. marmoratus in autumn, and between non-native species N. albiflora and native species H. otakii in winter. It was suggested the non-native species N. albiflora was the key species in the food competition among the six dominant fish species in this rocky reef habitat, and thus the feeding behaviors of these six fish species could have definite effects on the resource capacity of juvenile S. marmoratus.

  13. Exotic species enhance response diversity to land-use change but modify functional composition.

    PubMed

    Stavert, Jamie R; Pattemore, David E; Gaskett, Anne C; Beggs, Jacqueline R; Bartomeus, Ignasi

    2017-08-16

    Two main mechanisms may buffer ecosystem functions despite biodiversity loss. First, multiple species could share similar ecological roles, thus providing functional redundancy. Second, species may respond differently to environmental change (response diversity). However, ecosystem function would be best protected when functionally redundant species also show response diversity. This linkage has not been studied directly, so we investigated whether native and exotic pollinator species with similar traits (functional redundancy) differed in abundance (response diversity) across an agricultural intensification gradient. Exotic pollinator species contributed most positive responses, which partially stabilized overall abundance of the pollinator community. However, although some functionally redundant species exhibited response diversity, this was not consistent across functional groups and aggregate abundances within each functional group were rarely stabilized. This shows functional redundancy and response diversity do not always operate in concert. Hence, despite exotic species becoming increasingly dominant in human-modified systems, they cannot replace the functional composition of native species. © 2017 The Author(s).

  14. Cryptic Species Identification and Composition of Bemisia tabaci (Hemiptera: Aleyrodidae) Complex in Henan Province, China

    PubMed Central

    Hu, Jian; Wang, Lun-Ji; Dong, Jun-Feng; Song, Yue-Qin; Sun, Hui-Zhong

    2017-01-01

    Abstract Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex, causing significant crop losses in China during the last decade. Although knowledge of cryptic species composition and dynamics within B. tabaci complex is critical for developing sustainable pest management strategies, limited information is available on this pest in the Henan province of China. A systematic survey of the cryptic species composition and distribution of B. tabaci complex in different locations of Henan province was conducted in 2012. The results of RAPD-PCR and the gene for the mitochondrial cytochrome oxidase subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian method indicated there were four known cryptic species MEAM1, MED, Asia II 3, Asia II 9 and a new cryptic species named China 6 in Henan province. In the survey, the invasive cryptic species MED and MEAM1 were found to be predominant with wide spread distribution across the surveyed regions. On the contrary, the indigenous B. tabaci cryptic species including Asia II 3, Asia II 9 and China 6 remained with low prevalence in some surveyed regions. Cryptic species MEAM1 and MED have not completely displaced the native B. tabaci in Henan province. This current study for the first time unifies our knowledge of the diversity and distribution of B. tabaci across Henan province of China. PMID:28973577

  15. The relationship between total cholinesterase activity and mortality in four butterfly species

    USGS Publications Warehouse

    Bargar, Timothy A.

    2012-01-01

    The relationship between total cholinesterase activity (TChE) and mortality in four butterfly species (great southern white [Ascia monuste], common buckeye [Junonia coenia], painted lady [Vanessa cardui], and julia butterflies [Dryas julia]) was investigated. Acute contact toxicity studies were conducted to evaluate the response (median lethal dose [LD50] and TChE) of the four species following exposure to the organophosphate insecticide naled. The LD50 for these butterflies ranged from 2.3 to 7.6 μg/g. The average level of TChE inhibition associated with significant mortality ranged from 26 to 67%, depending on the species. The lower bounds of normal TChE activity (2 standard deviations less than the average TChE for reference butterflies) ranged from 8.4 to 12.3 μM/min/g. As a percentage of the average reference TChE activity for the respective species, the lower bounds were similar to the inhibition levels associated with significant mortality, indicating there was little difference between the dose resulting in significant TChE inhibition and that resulting in mortality.

  16. Nutrient and mineral composition during shoot growth in seven species of Phyllostachys and Pseudosasa bamboo consumed by giant panda.

    PubMed

    Christian, A L; Knott, K K; Vance, C K; Falcone, J F; Bauer, L L; Fahey, G C; Willard, S; Kouba, A J

    2015-12-01

    During the annual period of bamboo shoot growth in spring, free-ranging giant pandas feed almost exclusively on the shoots while ignoring the leaves and full- height culm. Little is known about the nutritional changes that occur during bamboo shoot growth, if nutritional changes differ among species, or how these changes might influence forage selection. Our objective was to examine the nutrient and mineral composition during three phases of shoot growth (<60, 90-150 and >180 cm) for seven species of bamboo (Phyllostachys (P.) aurea, P. aureosulcata, P. bissetii, P. glauca, P. nuda, P. rubromarginata, Pseudosasa japonica) fed to captive giant pandas at the Memphis Zoo. Total dietary fiber content of bamboo shoots increased (p < 0.0001) from an overall species average of 61% dry matter (DM) at < 60 cm to 75% DM at shoot heights > 180 cm, while crude protein, fat and ash exhibited significant declines (p < 0.05). Phyllostachys nuda had the overall greatest (p = 0.007) crude protein (21% DM) and fat (4% DM) content, and lowest overall total fibre (61% DM) content compared to the other species examined. In contrast, Pseudosasa japonica had the overall lowest crude protein and fat, and relatively higher fibre content (9%, 3% and 74% respectively). Concentrations of Zn and Fe were highest in shoots <60 cm (10-50 μg/g DM) and decreased (p < 0.05) during growth in all species examined. Concentrations of Ca, Cu, Mn, Na and K varied among species and were largely unaffected by growth stage. Due to their higher concentrations of nutrients and lower fibre content in comparison to culm and leaf, bamboo shoots should be a major component of captive giant panda diets when available. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  17. Differential effects of conifer and broadleaf litter inputs on soil organic carbon chemical composition through altered soil microbial community composition

    PubMed Central

    Wang, Hui; Liu, Shi-Rong; Wang, Jing-Xin; Shi, Zuo-Min; Xu, Jia; Hong, Pi-Zheng; Ming, An-Gang; Yu, Hao-Long; Chen, Lin; Lu, Li-Hua; Cai, Dao-Xiong

    2016-01-01

    A strategic selection of tree species will shift the type and quality of litter input, and subsequently magnitude and composition of the soil organic carbon (SOC) through soil microbial community. We conducted a manipulative experiment in randomized block design with leaf litter inputs of four native subtropical tree species in a Pinus massoniana plantation in southern China and found that the chemical composition of SOC did not differ significantly among treatments until after 28 months of the experiment. Contrasting leaf litter inputs had significant impacts on the amounts of total microbial, Gram-positive bacterial, and actinomycic PLFAs, but not on the amounts of total bacterial, Gram-negative bacterial, and fungal PLFAs. There were significant differences in alkyl/O-alkyl C in soils among the leaf litter input treatments, but no apparent differences in the proportions of chemical compositions (alkyl, O-alkyl, aromatic, and carbonyl C) in SOC. Soil alkyl/O-alkyl C was significantly related to the amounts of total microbial, and Gram-positive bacterial PLFAs, but not to the chemical compositions of leaf litter. Our findings suggest that changes in forest leaf litter inputs could result in changes in chemical stability of SOC through the altered microbial community composition. PMID:27256545

  18. Differential effects of conifer and broadleaf litter inputs on soil organic carbon chemical composition through altered soil microbial community composition.

    PubMed

    Wang, Hui; Liu, Shi-Rong; Wang, Jing-Xin; Shi, Zuo-Min; Xu, Jia; Hong, Pi-Zheng; Ming, An-Gang; Yu, Hao-Long; Chen, Lin; Lu, Li-Hua; Cai, Dao-Xiong

    2016-06-03

    A strategic selection of tree species will shift the type and quality of litter input, and subsequently magnitude and composition of the soil organic carbon (SOC) through soil microbial community. We conducted a manipulative experiment in randomized block design with leaf litter inputs of four native subtropical tree species in a Pinus massoniana plantation in southern China and found that the chemical composition of SOC did not differ significantly among treatments until after 28 months of the experiment. Contrasting leaf litter inputs had significant impacts on the amounts of total microbial, Gram-positive bacterial, and actinomycic PLFAs, but not on the amounts of total bacterial, Gram-negative bacterial, and fungal PLFAs. There were significant differences in alkyl/O-alkyl C in soils among the leaf litter input treatments, but no apparent differences in the proportions of chemical compositions (alkyl, O-alkyl, aromatic, and carbonyl C) in SOC. Soil alkyl/O-alkyl C was significantly related to the amounts of total microbial, and Gram-positive bacterial PLFAs, but not to the chemical compositions of leaf litter. Our findings suggest that changes in forest leaf litter inputs could result in changes in chemical stability of SOC through the altered microbial community composition.

  19. Differential effects of conifer and broadleaf litter inputs on soil organic carbon chemical composition through altered soil microbial community composition

    NASA Astrophysics Data System (ADS)

    Wang, Hui; Liu, Shi-Rong; Wang, Jing-Xin; Shi, Zuo-Min; Xu, Jia; Hong, Pi-Zheng; Ming, An-Gang; Yu, Hao-Long; Chen, Lin; Lu, Li-Hua; Cai, Dao-Xiong

    2016-06-01

    A strategic selection of tree species will shift the type and quality of litter input, and subsequently magnitude and composition of the soil organic carbon (SOC) through soil microbial community. We conducted a manipulative experiment in randomized block design with leaf litter inputs of four native subtropical tree species in a Pinus massoniana plantation in southern China and found that the chemical composition of SOC did not differ significantly among treatments until after 28 months of the experiment. Contrasting leaf litter inputs had significant impacts on the amounts of total microbial, Gram-positive bacterial, and actinomycic PLFAs, but not on the amounts of total bacterial, Gram-negative bacterial, and fungal PLFAs. There were significant differences in alkyl/O-alkyl C in soils among the leaf litter input treatments, but no apparent differences in the proportions of chemical compositions (alkyl, O-alkyl, aromatic, and carbonyl C) in SOC. Soil alkyl/O-alkyl C was significantly related to the amounts of total microbial, and Gram-positive bacterial PLFAs, but not to the chemical compositions of leaf litter. Our findings suggest that changes in forest leaf litter inputs could result in changes in chemical stability of SOC through the altered microbial community composition.

  20. The shark assemblage at French Frigate Shoals atoll, Hawai'i: species composition, abundance and habitat use.

    PubMed

    Dale, Jonathan J; Stankus, Austin M; Burns, Michael S; Meyer, Carl G

    2011-02-10

    Empirical data on the abundance and habitat preferences of coral reef top predators are needed to evaluate their ecological impacts and guide management decisions. We used longline surveys to quantify the shark assemblage at French Frigate Shoals (FFS) atoll from May to August 2009. Fishing effort consisted of 189 longline sets totaling 6,862 hook hours of soak time. A total of 221 sharks from 7 species were captured, among which Galapagos (Carcharhinus galapagensis, 36.2%), gray reef (Carcharhinus amblyrhynchos, 25.8%) and tiger (Galeocerdo cuvier, 20.4%) sharks were numerically dominant. A lack of blacktip reef sharks (Carcharhinus melanopterus) distinguished the FFS shark assemblage from those at many other atolls in the Indo-Pacific. Compared to prior underwater visual survey estimates, longline methods more accurately represented species abundance and composition for the majority of shark species. Sharks were significantly less abundant in the shallow lagoon than adjacent habitats. Recaptures of Galapagos sharks provided the first empirical estimate of population size for any Galapagos shark population. The overall recapture rate was 5.4%. Multiple closed population models were evaluated, with Chao M(h) ranking best in model performance and yielding a population estimate of 668 sharks with 95% confidence intervals ranging from 289-1720. Low shark abundance in the shallow lagoon habitats suggests removal of a small number of sharks from the immediate vicinity of lagoonal islets may reduce short-term predation on endangered monk seal (Monachus schauinslandi) pups, but considerable fishing effort would be required to catch even a small number of sharks. Additional data on long-term movements and habitat use of sharks at FFS are required to better assess the likely ecological impacts of shark culling.

  1. Seasonal variation in species composition and abundance of demersal fish and invertebrates in a Seagrass Natural Reserve on the eastern coast of the Shandong Peninsula, China

    NASA Astrophysics Data System (ADS)

    Xu, Qiang; Guo, Dong; Zhang, Peidong; Zhang, Xiumei; Li, Wentao; Wu, Zhongxin

    2016-03-01

    Seagrass habitats are structurally complex ecosystems, which support high productivity and biodiversity. In temperate systems the density of seagrass may change seasonally, and this may influence the associated fish and invertebrate community. Little is known about the role of seagrass beds as possible nursery areas for fish and invertebrates in China. To study the functioning of a seagrass habitat in northern China, demersal fish and invertebrates were collected monthly using traps, from February 2009 to January 2010. The density, leaf length and biomass of the dominant seagrass Zostera marina and water temperature were also measured. The study was conducted in a Seagrass Natural Reserve (SNR) on the eastern coast of the Shandong Peninsula, China. A total of 22 fish species and five invertebrate species were recorded over the year. The dominant fish species were Synechogobius ommaturus, Sebastes schlegelii, Pholis fangi, Pagrus major and Hexagrammos otakii and these species accounted for 87% of the total number of fish. The dominant invertebrate species were Charybdis japonica and Octopus variabilis and these accounted for 98% of the total abundance of invertebrates. There was high temporal variation in species composition and abundance. The peak number of fish species occurred in August-October 2009, while the number of individual fish and biomass was highest during November 2009. Invertebrate numbers and biomass was highest in March, April, July and September 2009. Temporal changes in species abundance of fishes and invertebrates corresponded with changes in the shoot density and leaf length of the seagrass, Zostera marina.

  2. Nutrient addition shifts plant community composition towards earlier flowering species in some prairie ecoregions in the U.S. Central Plains.

    PubMed

    Biederman, Lori; Mortensen, Brent; Fay, Philip; Hagenah, Nicole; Knops, Johannes; La Pierre, Kimberly; Laungani, Ramesh; Lind, Eric; McCulley, Rebecca; Power, Sally; Seabloom, Eric; Tognetti, Pedro

    2017-01-01

    The distribution of flowering across the growing season is governed by each species' evolutionary history and climatic variability. However, global change factors, such as eutrophication and invasion, can alter plant community composition and thus change the distribution of flowering across the growing season. We examined three ecoregions (tall-, mixed, and short-grass prairie) across the U.S. Central Plains to determine how nutrient (nitrogen (N), phosphorus, and potassium (+micronutrient)) addition alters the temporal patterns of plant flowering traits. We calculated total community flowering potential (FP) by distributing peak-season plant cover values across the growing season, allocating each species' cover to only those months in which it typically flowers. We also generated separate FP profiles for exotic and native species and functional group. We compared the ability of the added nutrients to shift the distribution of these FP profiles (total and sub-groups) across the growing season. In all ecoregions, N increased the relative cover of both exotic species and C3 graminoids that flower in May through August. The cover of C4 graminoids decreased with added N, but the response varied by ecoregion and month. However, these functional changes only aggregated to shift the entire community's FP profile in the tall-grass prairie, where the relative cover of plants expected to flower in May and June increased and those that flower in September and October decreased with added N. The relatively low native cover in May and June may leave this ecoregion vulnerable to disturbance-induced invasion by exotic species that occupy this temporal niche. There was no change in the FP profile of the mixed and short-grass prairies with N addition as increased abundance of exotic species and C3 graminoids replaced other species that flower at the same time. In these communities a disturbance other than nutrient addition may be required to disrupt phenological patterns.

  3. Phytotoxic Activity and Chemical Composition of Aqueous Volatile Fractions from Eucalyptus Species

    PubMed Central

    Zhang, Jinbiao; An, Min; Wu, Hanwen; Liu, De Li; Stanton, Rex

    2014-01-01

    The essential oils from four Eucalyptus species (E. spathulata, E. salubris, E. brockwayii and E. dundasii) have been previously confirmed to have stronger inhibitory effects on germination and seedling growth of silverleaf nightshade (Solanum elaeagnifolium Cav.). The aqueous volatile fractions (AVFs) were the water soluble volatile fractions produced together with the essential oils (water insoluble fractions) during the steam distillation process. The aim of this study was to further assess the phytotoxicity of AVFs from the four Eucalyptus species and their chemical composition. The fresh leaves of the four Eucalyptus species were used for the extraction of AVFs. The AVFs were tested for their phytotoxic effects on the perennial weed, silverleaf nightshade under laboratory conditions. The chemical compositions of the AVFs were determined by gas chromatograph–mass spectrometry (GC-MS). Our results showed that the AVFs had strong inhibition on the germination and seedling growth of silverleaf nightshade. The inhibition index increased with the increasing concentrations of AVFs. The inhibitory effects of the AVFs varied between different Eucalyptus species. The AVF from E. salubris demonstrated the highest inhibitory activity on the weed tested, with complete inhibition on germination and seedling growth at a concentration of 75%. The GC-MS analysis revealed that 1,8-cineole, isopentyl isovalerate, isomenthol, pinocarvone, trans-pinocarveol, alpha-terpineol and globulol were the main compounds in the AVFs. These results indicated that all AVFs tested had differential inhibition on the germination and seedling growth of silverleaf nightshade, which could be due to the joint effects of compounds present in the AVFs as these compounds were present in different quantities and ratio between Eucalyptus species. PMID:24681490

  4. Species composition of the international shark fin trade assessed through a retail-market survey in Hong Kong.

    PubMed

    Fields, Andrew T; Fischer, Gunter A; Shea, Stanley K H; Zhang, Huarong; Abercrombie, Debra L; Feldheim, Kevin A; Babcock, Elizabeth A; Chapman, Demian D

    2018-04-01

    The shark fin trade is a major driver of shark exploitation in fisheries all over the world, most of which are not managed on a species-specific basis. Species-specific trade information highlights taxa of particular concern and can be used to assess the efficacy of management measures and anticipate emerging threats. The species composition of the Hong Kong Special Administrative Region of China, one of the world's largest fin trading hubs, was partially assessed in 1999-2001. We randomly selected and genetically identified fin trimmings (n = 4800), produced during fin processing, from the retail market of Hong Kong in 2014-2015 to assess contemporary species composition of the fin trade. We used nonparametric species estimators to determine that at least 76 species of sharks, batoids, and chimaeras supplied the fin trade and a Bayesian model to determine their relative proportion in the market. The diversity of traded species suggests species substitution could mask depletion of vulnerable species; one-third of identified species are threatened with extinction. The Bayesian model suggested that 8 species each comprised >1% of the fin trimmings (34.1-64.2% for blue [Prionace glauca], 0.2-1.2% for bull [Carcharhinus leucas] and shortfin mako [Isurus oxyrinchus]); thus, trade was skewed to a few globally distributed species. Several other coastal sharks, batoids, and chimaeras are in the trade but poorly managed. Fewer than 10 of the species we modeled have sustainably managed fisheries anywhere in their range, and the most common species in trade, the blue shark, was not among them. Our study and approach serve as a baseline to track changes in composition of species in the fin trade over time to better understand patterns of exploitation and assess the effects of emerging management actions for these animals. © 2017 The Authors. Conservation Biology published by Wiley Periodicals, Inc. on behalf of Society for Conservation Biology.

  5. Tree hole mosquito species composition and relative abundances differ between urban and adjacent forest habitats in northwestern Argentina.

    PubMed

    Mangudo, C; Aparicio, J P; Rossi, G C; Gleiser, R M

    2018-04-01

    Water-holding tree holes are main larval habitats for many pathogen vectors, especially mosquitoes (Diptera: Culicidae). Along 3 years, the diversity and composition of mosquito species in tree holes of two neighbouring but completely different environments, a city and its adjacent forest, were compared using generalized linear mixed models, PERMANOVA, SIMPER and species association indexes. The city area (Northwest Argentina) is highly relevant epidemiologically due to the presence of Aedes aegypti L. (main dengue vector) and occurrence of dengue outbreaks; the Yungas rainforests are highly biologically diverse. In total seven mosquito species were recorded, in descending order of abundance: Ae. aegypti, Haemagogus spegazzinii Brèthes, Sabethes purpureus (Theobald), Toxorhynchites guadeloupensis Dyar and Knab, Aedes terrens Walker, Haemagogus leucocelaenus Dyar & Shannon and Sabethes petrocchiae (Shannon and Del Ponte). The seven mosquito species were recorded in both city sites and forested areas; however, their mosquito communities significantly diverged because of marked differences in the frequency and relative abundance of some species: Tx. guadeloupensis and Ae. aegypti were significantly more abundant in forest and urban areas, respectively. Positive significant associations were detected between Ae. aegypti, Hg. spegazzinii and Hg. leucocelaenus. The combined presence of Ae. aegypti, Haemagogus and Sabethes in the area also highlight a potential risk of yellow fever epidemics. Overall results show an impoverished tree hole mosquito fauna in urban environments, reflecting negative effects of urbanization on mosquito diversity.

  6. Evolution of Active Sites in Pt-Based Nanoalloy Catalysts for the Oxidation of Carbonaceous Species by Combined in Situ Infrared Spectroscopy and Total X-ray Scattering.

    PubMed

    Petkov, Valeri; Maswadeh, Yazan; Lu, Aolin; Shan, Shiyao; Kareem, Haval; Zhao, Yinguang; Luo, Jin; Zhong, Chuan-Jian; Beyer, Kevin; Chapman, Karena

    2018-04-04

    We present results from combined in situ infrared spectroscopy and total X-ray scattering studies on the evolution of catalytically active sites in exemplary binary and ternary Pt-based nanoalloys during a sequence of CO oxidation-reactivation-CO oxidation reactions. We find that when within a particular compositional range, the fresh nanoalloys may exhibit high catalytic activity for low-temperature CO oxidation. Using surface-specific atomic pair distribution functions (PDFs) extracted from the in situ total X-ray scattering data, we find that, regardless of their chemical composition and initial catalytic activity, the fresh nanoalloys suffer a significant surface structural disorder during CO oxidation. Upon reactivation in oxygen atmosphere, the surface of used nanoalloy catalysts both partially oxidizes and orders. Remarkably, it largely retains its structural state when the nanoalloys are reused as CO oxidation catalysts. The seemingly inverse structural changes of studied nanoalloy catalysts occurring under CO oxidation and reactivation conditions affect the active sites on their surface significantly. In particular, through different mechanisms, both appear to reduce the CO binding strength to the nanoalloy's surface and thus increase the catalytic stability of the nanoalloys. The findings provide clues for further optimization of nanoalloy catalysts for the oxidation of carbonaceous species through optimizing their composition, activation, and reactivation. Besides, the findings demonstrate the usefulness of combined in situ infrared spectroscopy and total X-ray scattering coupled to surface-specific atomic PDF analysis to the ongoing effort to produce advanced catalysts for environmentally and technologically important applications.

  7. [Species composition and distribution patterns of ichthyoplankton within and outside artificial reefs in Qingshan Bay, Qingdao, China].

    PubMed

    Guo, Shu Xin; Gao, Dong Kui; Zhang, Xiu Mei; Li, Wen Tao; Zhang, Pei Dong

    2017-06-18

    To assess the fish attraction and shelter effects of the artificial reefs in Qingshan Bay of Qingdao, the species composition and distribution patterns of ichthyoplankton in artificial reefs were investigated using vertical and horizontal tows in 2014 and 2015. In total, 7306 fish eggs and 52 fish larvae, belonging to 4 orders, 9 families, 11 genera and 12 species, were collected during 7 cruises in spring, summer and autumn of 2014. In 2015, 10373 eggs and 159 fish larvae, belonging to 6 orders, 11 families, 14 genera and 15 species, were collected in the same period as in 2014. Perciformes were the majority for both fish eggs and larvae collected during the two surveys, followed by Pleuronectiformes. Among fish eggs, Sillago japonica and Cynoglossus joyneri were the most dominant species. Among fish larvae, S. japonica exhibited the highest dominance but was not the dominant species. The high dominant species in both fish eggs and larvae appeared alternately in diffe-rent seasons. The Margalef richness index (R), Shannon diversity index (H) and Pielou evenness index (J) of ichthyoplankton community were low during the spring, summer, and autumn, showing an instable community structure of ichthyoplankton. The average trophic level of adult fish corresponding to the ichthyoplankton collected in the two years were 3.71 and 3.78, respectively, and both belonged to low carnivorous fish of third trophic level. All the species were either warm-tempe-rate species or warm-water species, which was coincident with the warm-temperate zone characteristic. Comprehensive analysis showed that the biodiversity of the ichthyoplankton community within the artificial reef areas was higher than that outside the artificial reef areas, which might be linked to flow velocity, flow field characteristics, or spatial heterogeneity of artificial reef areas.

  8. [Responses of plant community structure and species composition to warming and N addition in an alpine meadow, northern Tibetan Plateau, China].

    PubMed

    Zong, Ning; Chai, Xi; Shi, Pei Li; Jiang, Jing; Niu, Ben; Zhang, Xian Zhou; He, Yong Tao

    2016-12-01

    Global climate warming and increasing nitrogen (N) deposition, as controversial global environmental issues, may distinctly affect the functions and processes of terrestrial ecosystems. It has been reported that the Qinghai-Tibet Plateau has been experiencing significant warming in recent decades, especially in winter. Previous studies have mainly focused on the effects of warming all the year round; however, few studies have tested the effects of winter warming. To investigate the effects of winter warming and N addition on plant community structure and species composition of alpine meadow, long-term N addition and simulated warming experiment was conducted in alpine meadow from 2010 in Damxung, northern Tibet. The experiment consisted of three warming patterns: Year-round warming (YW), winter warming (WW) and control (NW), crossed respectively with five N gradients: 0, 10, 20, 40, 80 kg N·hm -2 ·a -1 . From 2012 to 2014, both warming and N addition significantly affected the total coverage of plant community. Specifically, YW significantly decreased the total coverage of plant community. Without N addition, WW remarkably reduced the vegetation coverage. However, with N addition, the total vegetation coverage gradually increased with the increase of N level. Warming and N addition had different effects on plants from different functional groups. Warming significantly reduced the plant coverage of grasses and sedges, while N addition significantly enhanced the plant coverage of grasses. Regression analyses showed that the total coverage of plant community was positively related to soil water content in vigorous growth stages, indicating that the decrease in soil water content resulted from warming during dry seasons might be the main reason for the decline of total community coverage. As soil moisture in semi-arid alpine meadow is mainly regulated by rainfalls, our results indicated that changes in spatial and temporal patterns of rainfalls under the future

  9. Changes in plant species composition of coastal dune habitats over a 20-year period

    PubMed Central

    Del Vecchio, Silvia; Prisco, Irene; Acosta, Alicia T. R.; Stanisci, Angela

    2015-01-01

    Coastal sandy ecosystems are increasingly being threatened by human pressure, causing loss of biodiversity, habitat degradation and landscape modifications. However, there are still very few detailed studies focussing on compositional changes in coastal dune plant communities over time. In this work, we investigated how coastal dune European Union (EU) habitats (from pioneer annual beach communities to Mediterranean scrubs on the landward fixed dunes) have changed during the last 20 years. Using phytosociological relevés conducted in 1989–90 and in 2010–12, we investigated changes in floristic composition over time. We then compared plant cover and the proportion of ruderal, alien and habitat diagnostic species (‘focal species’) in the two periods. Finally, we used Ellenberg indicator values to define the ‘preferences’ of the plant species for temperature and moisture. We found that only fore dune habitats showed significant differences in species cover between the two time periods, with higher plant cover in the more recent relevés and a significant increase in thermophilic species. Although previous studies have demonstrated consistent habitat loss in this area, we observed that all coastal dune plant communities remain well represented, after a 20-year period. However, fore dunes have been experiencing significant compositional changes. Although we cannot confirm whether the observed changes are strictly related to climatic changes, to human pressure or to both, we hypothesize that a moderate increment in average yearly temperature may have promoted the increase in plant cover and the spread of thermophilic species. Thus, even though human activities are major driving forces of change in coastal dune vegetation, at the community scale climatic factors may also play important roles. Our study draws on re-visitation studies which appear to constitute a powerful tool for the assessment of the conservation status of EU habitats. PMID:25750408

  10. Factors affecting plant species composition of hedgerows: relative importance and hierarchy

    NASA Astrophysics Data System (ADS)

    Deckers, Bart; Hermy, Martin; Muys, Bart

    2004-07-01

    Although there has been a clear quantitative and qualitative decline in traditional hedgerow network landscapes during last century, hedgerows are crucial for the conservation of rural biodiversity, functioning as an important habitat, refuge and corridor for numerous species. To safeguard this conservation function, insight in the basic organizing principles of hedgerow plant communities is needed. The vegetation composition of 511 individual hedgerows situated within an ancient hedgerow network landscape in Flanders, Belgium was recorded, in combination with a wide range of explanatory variables, including a selection of spatial variables. Non-parametric statistics in combination with multivariate data analysis techniques were used to study the effect of individual explanatory variables. Next, variables were grouped in five distinct subsets and the relative importance of these variable groups was assessed by two related variation partitioning techniques, partial regression and partial canonical correspondence analysis, taking into account explicitly the existence of intercorrelations between variables of different factor groups. Most explanatory variables affected significantly hedgerow species richness and composition. Multivariate analysis showed that, besides adjacent land use, hedgerow management, soil conditions, hedgerow type and origin, the role of other factors such as hedge dimensions, intactness, etc., could certainly not be neglected. Furthermore, both methods revealed the same overall ranking of the five distinct factor groups. Besides a predominant impact of abiotic environmental conditions, it was found that management variables and structural aspects have a relatively larger influence on the distribution of plant species in hedgerows than their historical background or spatial configuration.

  11. Cryptic Species Identification and Composition of Bemisia tabaci (Hemiptera: Aleyrodidae) Complex in Henan Province, China.

    PubMed

    Jiu, Min; Hu, Jian; Wang, Lun-Ji; Dong, Jun-Feng; Song, Yue-Qin; Sun, Hui-Zhong

    2017-05-01

    Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex, causing significant crop losses in China during the last decade. Although knowledge of cryptic species composition and dynamics within B. tabaci complex is critical for developing sustainable pest management strategies, limited information is available on this pest in the Henan province of China. A systematic survey of the cryptic species composition and distribution of B. tabaci complex in different locations of Henan province was conducted in 2012. The results of RAPD-PCR and the gene for the mitochondrial cytochrome oxidase subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian method indicated there were four known cryptic species MEAM1, MED, Asia II 3, Asia II 9 and a new cryptic species named China 6 in Henan province. In the survey, the invasive cryptic species MED and MEAM1 were found to be predominant with wide spread distribution across the surveyed regions. On the contrary, the indigenous B. tabaci cryptic species including Asia II 3, Asia II 9 and China 6 remained with low prevalence in some surveyed regions. Cryptic species MEAM1 and MED have not completely displaced the native B. tabaci in Henan province. This current study for the first time unifies our knowledge of the diversity and distribution of B. tabaci across Henan province of China. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America.

  12. Spatial heterogeneity in species composition constrains plant community responses to herbivory and fertilization

    USDA-ARS?s Scientific Manuscript database

    Changing environmental conditions result in substantial shifts in the composition of communities. The associated immigration and extinction events are likely constrained by the spatial distribution of species. Still, most studies on environmental change quantify the biotic responses at single spat...

  13. Influences of Species Interactions With Aggressive Ants and Habitat Filtering on Nest Colonization and Community Composition of Arboreal Twig-Nesting Ants.

    PubMed

    Philpott, Stacy M; Serber, Zachary; De la Mora, Aldo

    2018-04-05

    Ant community assembly is driven by many factors including species interactions (e.g., competition, predation, parasitism), habitat filtering (e.g., vegetation differences, microclimate, food and nesting resources), and dispersal. Canopy ant communities, including dominant and twig-nesting ants, are structured by all these different factors, but we know less about the impacts of species interactions and habitat filters acting at the colonization or recruitment stage. We examined occupation of artificial twig nests placed in shade trees in coffee agroecosystems. We asked whether species interactions-aggression from the dominant canopy ant, Azteca sericeasur Longino (Hymenoptera: Formicidae)-or habitat filtering-species of tree where nests were placed or surrounding vegetation-influence colonization, species richness, and community composition of twig-nesting ants. We found 20 species of ants occupying artificial nests. Nest occupation was lower on trees with A. sericeasur, but did not differ depending on tree species or surrounding vegetation. Yet, there were species-specific differences in occupation depending on A. sericeasur presence and tree species. Ant species richness did not vary with A. sericeasur presence or tree species. Community composition varied with A. sericeasur presence and surrounding vegetation. Our results suggest that species interactions with dominant ants are important determinants of colonization and community composition of twig-nesting ants. Habitat filtering at the level of tree species did not have strong effects on twig-nesting ants, but changes in coffee management may contribute to differences in community composition with important implications for ant conservation in agricultural landscapes, as well as biological control of coffee pests.

  14. Tocochromanol content and composition in different species of the parasitic flowering plant genus Cuscuta.

    PubMed

    van der Kooij, Thomas A W; Krupinska, Karin; Krause, Kirsten

    2005-07-01

    The holoparasitic plant genus Cuscuta is comprised of species with various degrees of plastid functionality and significant differences in photosynthetic capacity, ranging from moderate to no photosynthetic carbon fixation. In the present study, several Cuscuta species were analyzed with respect to the overall contents of tocochromanols and plastoquinone and the levels of the individual tocochromanols. No correlations among photosynthetic capacity, the amount of carotenoids, of plastoquinone and of tocochromanols were observed. On the contrary, wide variation in the composition of the tocochromanol fraction was observed among different species, as well as in stems of the same species in response to starvation conditions. The implications of these findings are discussed.

  15. Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Coleman-Derr, Devin; Desgarennes, Damaris; Fonseca-Garcia, Citlali

    Desert plants are hypothesized to survive the environmental stress inherent to these regions in part thanks to symbioses with microorganisms, and yet these microbial species, the communities they form, and the forces that influence them are poorly understood. Here we report the first comprehensive investigation of the microbial communities associated with species of Agave, which are native to semiarid and arid regions of Central and North America and are emerging as biofuel feedstocks. We examined prokaryotic and fungal communities in the rhizosphere, phyllosphere, leaf and root endosphere, as well as proximal and distal soil samples from cultivated and native agaves,more » through Illumina amplicon sequencing. Phylogenetic profiling revealed that the composition of prokaryotic communities was primarily determined by the plant compartment, whereas the composition of fungal communities was mainly influenced by the biogeography of the host species. Cultivated A. tequilana exhibited lower levels of prokaryotic diversity compared with native agaves, although no differences in microbial diversity were found in the endosphere. Agaves shared core prokaryotic and fungal taxa known to promote plant growth and confer tolerance to abiotic stress, which suggests common principles underpinning Agave-microbe interactions.« less

  16. Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species

    DOE PAGES

    Coleman-Derr, Devin; Desgarennes, Damaris; Fonseca-Garcia, Citlali; ...

    2015-10-15

    Desert plants are hypothesized to survive the environmental stress inherent to these regions in part thanks to symbioses with microorganisms, and yet these microbial species, the communities they form, and the forces that influence them are poorly understood. Here we report the first comprehensive investigation of the microbial communities associated with species of Agave, which are native to semiarid and arid regions of Central and North America and are emerging as biofuel feedstocks. We examined prokaryotic and fungal communities in the rhizosphere, phyllosphere, leaf and root endosphere, as well as proximal and distal soil samples from cultivated and native agaves,more » through Illumina amplicon sequencing. Phylogenetic profiling revealed that the composition of prokaryotic communities was primarily determined by the plant compartment, whereas the composition of fungal communities was mainly influenced by the biogeography of the host species. Cultivated A. tequilana exhibited lower levels of prokaryotic diversity compared with native agaves, although no differences in microbial diversity were found in the endosphere. Agaves shared core prokaryotic and fungal taxa known to promote plant growth and confer tolerance to abiotic stress, which suggests common principles underpinning Agave-microbe interactions.« less

  17. Amino acid and proximate composition of fish bone gelatin from different warm-water species: A comparative study

    NASA Astrophysics Data System (ADS)

    Atma, Y.

    2017-03-01

    Research on fish bone gelatin has been increased in the last decade. The quality of gelatin depends on its physicochemical properties. Fish bone gelatin from warm-water fishes has a superior amino acid composition than cold-water fishes. The composition of amino acid can determine the strength and stability of gelatin. Thus, it is important to analyze the composition of amino acid as well as proximate composition for potential gelatin material. The warm water fish species used in this study were Grass carp, Pangasius catfish, Catfish, Lizard fish, Tiger-toothed croaker, Pink perch, Red snapper, Brown spotted grouper, and King weakfish. There werre five dominant amino acid in fish bone gelatin including glycine (21.2-36.7%), proline (8.7-11.7%), hydroxyproline (5.3-9.6%), alanine (8.48-12.9%), and glutamic acid (7.23-10.15%). Different warm-water species has some differences in amino acid composition. The proximate composition showed that fishbone gelatin from Pangasius catfish has the highest protein content. The water composition of all fishbone gelatin was well suited to the standard. Meanwhile, based on ash content, only gelatin from gelatin Pangasius catfish met the standard for food industries.

  18. The mercury species and their association with carbonaceous compositions, bromine and iodine in PM2.5 in Shanghai.

    PubMed

    Duan, Lian; Xiu, Guangli; Feng, Ling; Cheng, Na; Wang, Chenggang

    2016-03-01

    PM2.5 samples were collected in south Shanghai from November 2013 to October 2014. The species of particulate bounded mercury (PBM), including hydrochloric soluble particle-phase mercury (HPM), element soluble particle-phase mercury (EPM) and residual soluble particle-phase mercury (RPM), were determined in PM2.5. The chemical composition of PM2.5 including organic carbon (OC) and elemental carbon (EC), total bromine and iodine were also analyzed. The results showed that the annual average concentration of PBM was 0.30 ± 0.31 ng m(-3) and 0.34 ± 0.32 ng m(-3) in winter, 0.31 ± 0.19 ng m(-3) in spring, 0.30 ± 0.45 ng m(-3) in fall and 0.28 ± 0.17 ng m(-3) in summer. HPM took the highest fraction 51.2% in PBM, followed by RPM 27.7% and EPM 21.1%. EC positively correlated to particle mercury, especially in winter (r = 0.70), the same for OC in winter (r = 0.72), which indicated that the carbonaceous composition may affect the transformation of Hg in the atmosphere. Mercury species showed different correlations with bromine and iodine in the four seasons. The strongest correlation between bromine, iodine and mercury was found in spring and fall, respectively. Bromine showed the stronger correlation with total mercury and speciated particle mercury than iodine. In addition, the days were classified into haze and non-haze days based on the visibility and relative humidity, while the ratio of HPM in haze days was much higher than that in non-haze days. EC strongly correlated with PBM during haze and non-haze days while OC only positively correlated with PBM in non-haze days, this may indicate that the different carbonaceous part may affect PBM differently. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. Species composition and relative abundance of sand flies of the genus Lutzomyia (Diptera: Psychodidae) at an endemic focus of visceral leishmaniasis in Colombia.

    PubMed

    Ferro, C; Morrison, A C; Torres, M; Pardo, R; Wilson, M L; Tesh, R B

    1995-07-01

    Ecological studies on the sand fly Lutzomyia longipalpis (Lutz & Neiva) were conducted during 1990-1993 at a small rural community in Colombia where American visceral leishmaniasis is endemic. Weekly sand fly collections were made from pigpens, houses, and natural resting sites, using hand-held aspirators, sticky (oiled) paper traps, and opossum-baited Disney traps. In total, 263,094 sand flies were collected; L. longipalpis predominated (86.1%), followed by L. trinidadensis (11.0%), L. cayennensis (2.7%), and 8 other Lutzomyia species. The species composition and sex ratio of these sand flies varied among sites and by collection method. L. longipalpis were captured most efficiently by direct aspiration from animal bait. Conversely, sticky paper traps, especially inside houses and at rock resting sites, collected a greater diversity of species, but a lower relative abundance of L. longipalpis.

  20. Chemical composition of some wild peanut species (Arachis L.) seeds.

    PubMed

    Grosso, N R; Nepote, V; Guzmán, C A

    2000-03-01

    Oil, protein, ash, and carbohydrate contents, iodine value, and fatty acid and sterol compositions were studied in seeds of Arachis trinitensis, A. chiquitana, A. kempff-mercadoi, A. diogoi, A. benensis, A. appressipila, A. valida, A. kretschmeri, A. helodes, A. kuhlmannii, A. williamsii, A. sylvestris, A. matiensis, A. pintoi, A. hoehnei, A. villosa, and A. stenosperma. Oil content was greatest in A.stenosperma (mean value = 51.8%). The protein level was higher in A. sylvestris (30.1%) and A. villosa (29.5%). Mean value of oleic acid varied between 30.6% (A. matiensis) and 46.8% (Arachis villosa), and linoleic acid oscillated between 34.1% (A. villosa) and 47.4% (A. appressipila). The better oleic-to-linoleic (O/L) ratio was exhibited by A. villosa (1.38). Some species showed higher concentration of behenic acid. The greatest level of this fatty acid was found in A. matiensis (6.2%). Iodine value was lower in A. valida (99.2). The sterol composition in the different peanut species showed higher concentration of beta-sitosterol (mean values oscillated between 55.7 and 60.2%) followed by campesterol (12.4-16. 5%), stigmasterol (9.7-13.3%), and Delta(5)-avenasterol (9.7-13.4%). The chemical quality and stability of oils (iodine value and O/L ratio) from wild peanut studied in this work are not better than those of cultivated peanut.

  1. Species richness and trait composition of butterfly assemblages change along an altitudinal gradient.

    PubMed

    Leingärtner, Annette; Krauss, Jochen; Steffan-Dewenter, Ingolf

    2014-06-01

    Species richness patterns along altitudinal gradients are well-documented ecological phenomena, yet very little data are available on how environmental filtering processes influence the composition and traits of butterfly assemblages at high altitudes. We have studied the diversity patterns of butterfly species at 34 sites along an altitudinal gradient ranging from 600 to 2,000 m a.s.l. in the National Park Berchtesgaden (Germany) and analysed traits of butterfly assemblages associated with dispersal capacity, reproductive strategies and developmental time from lowlands to highlands, including phylogenetic analyses. We found a linear decline in butterfly species richness along the altitudinal gradient, but the phylogenetic relatedness of the butterfly assemblages did not increase with altitude. Compared to butterfly assemblages at lower altitudes, those at higher altitudes were composed of species with larger wings (on average 9%) which laid an average of 68% more eggs. In contrast, egg maturation time in butterfly assemblages decreased by about 22% along the altitudinal gradient. Further, butterfly assemblages at higher altitudes were increasingly dominated by less widespread species. Based on our abundance data, but not on data in the literature, population density increased with altitude, suggesting a reversed density-distribution relationship, with higher population densities of habitat specialists in harsh environments. In conclusion, our data provide evidence for significant shifts in the composition of butterfly assemblages and for the dominance of different traits along the altitudinal gradient. In our study, these changes were mainly driven by environmental factors, whereas phylogenetic filtering played a minor role along the studied altitudinal range.

  2. Species composition and forest structure explain the temperature sensitivity patterns of productivity in temperate forests

    NASA Astrophysics Data System (ADS)

    Bohn, Friedrich J.; May, Felix; Huth, Andreas

    2018-03-01

    Rising temperatures due to climate change influence the wood production of forests. Observations show that some temperate forests increase their productivity, whereas others reduce their productivity. This study focuses on how species composition and forest structure properties influence the temperature sensitivity of aboveground wood production (AWP). It further investigates which forests will increase their productivity the most with rising temperatures. We described forest structure by leaf area index, forest height and tree height heterogeneity. Species composition was described by a functional diversity index (Rao's Q) and a species distribution index (ΩAWP). ΩAWP quantified how well species are distributed over the different forest layers with regard to AWP. We analysed 370 170 forest stands generated with a forest gap model. These forest stands covered a wide range of possible forest types. For each stand, we estimated annual aboveground wood production and performed a climate sensitivity analysis based on 320 different climate time series (of 1-year length). The scenarios differed in mean annual temperature and annual temperature amplitude. Temperature sensitivity of wood production was quantified as the relative change in productivity resulting from a 1 °C rise in mean annual temperature or annual temperature amplitude. Increasing ΩAWP positively influenced both temperature sensitivity indices of forest, whereas forest height showed a bell-shaped relationship with both indices. Further, we found forests in each successional stage that are positively affected by temperature rise. For such forests, large ΩAWP values were important. In the case of young forests, low functional diversity and small tree height heterogeneity were associated with a positive effect of temperature on wood production. During later successional stages, higher species diversity and larger tree height heterogeneity were an advantage. To achieve such a development, one could plant

  3. Contrasting patterns of fine-scale herb layer species composition in temperate forests

    NASA Astrophysics Data System (ADS)

    Chudomelová, Markéta; Zelený, David; Li, Ching-Feng

    2017-04-01

    Although being well described at the landscape level, patterns in species composition of forest herb layer are rarely studied at smaller scales. Here, we examined fine-scale environmental determinants and spatial structures of herb layer communities in thermophilous oak- and hornbeam dominated forests of the south-eastern part of the Czech Republic. Species composition of herb layer vegetation and environmental variables were recorded within a fixed grid of 2 × 2 m subplots regularly distributed within 1-ha quadrate plots in three forest stands. For each site, environmental models best explaining species composition were constructed using constrained ordination analysis. Spatial eigenvector mapping was used to model and account for spatial structures in community variation. Mean Ellenberg indicator values calculated for each subplot were used for ecological interpretation of spatially structured residual variation. The amount of variation explained by environmental and spatial models as well as the selection of variables with the best explanatory power differed among sites. As an important environmental factor, relative elevation was common to all three sites, while pH and canopy openness were shared by two sites. Both environmental and community variation was mostly coarse-scaled, as was the spatially structured portion of residual variation. When corrected for bias due to spatial autocorrelation, those environmental factors with already weak explanatory power lost their significance. Only a weak evidence of possibly omitted environmental predictor was found for autocorrelated residuals of site models using mean Ellenberg indicator values. Community structure was determined by different factors at different sites. The relative importance of environmental filtering vs. spatial processes was also site specific, implying that results of fine-scale studies tend to be shaped by local conditions. Contrary to expectations based on other studies, overall dominance of

  4. Species composition, larval habitats, seasonal occurrence and distribution of potential malaria vectors and associated species of Anopheles (Diptera: Culicidae) from the Republic of Korea

    PubMed Central

    2010-01-01

    Background Larval mosquito habitats of potential malaria vectors and related species of Anopheles from three provinces (Gyeonggi, Gyeongsangbuk, Chungcheongbuk Provinces) of the Republic of Korea were surveyed in 2007. This study aimed to determine the species composition, seasonal occurrence and distributions of Anopheles mosquitoes. Satellite derived normalized difference vegetation index data (NDVI) was also used to study the seasonal abundance patterns of Anopheles mosquitoes. Methods Mosquito larvae from various habitats were collected using a standard larval dipper or a white plastic larval tray, placed in plastic bags, and were preserved in 100% ethyl alcohol for species identification by PCR and DNA sequencing. The habitats in the monthly larval surveys included artificial containers, ground depressions, irrigation ditches, drainage ditches, ground pools, ponds, rice paddies, stream margins, inlets and pools, swamps, and uncultivated fields. All field-collected specimens were identified to species, and relationships among habitats and locations based on species composition were determined using cluster statistical analysis. Results In about 10,000 specimens collected, eight species of Anopheles belonging to three groups were identified: Hyrcanus Group - Anopheles sinensis, Anopheles kleini, Anopheles belenrae, Anopheles pullus, Anopheles lesteri, Anopheles sineroides; Barbirostris Group - Anopheles koreicus; and Lindesayi Group - Anopheles lindesayi japonicus. Only An. sinensis was collected from all habitats groups, while An. kleini, An. pullus and An. sineroides were sampled from all, except artificial containers. The highest number of Anopheles larvae was found in the rice paddies (34.8%), followed by irrigation ditches (23.4%), ponds (17.0%), and stream margins, inlets and pools (12.0%). Anopheles sinensis was the dominant species, followed by An. kleini, An. pullus and An. sineroides. The monthly abundance data of the Anopheles species from three

  5. Total centromere size and genome size are strongly correlated in ten grass species.

    PubMed

    Zhang, Han; Dawe, R Kelly

    2012-05-01

    It has been known for decades that centromere size varies across species, but the factors involved in setting centromere boundaries are unknown. As a means to address this question, we estimated centromere sizes in ten species of the grass family including rice, maize, and wheat, which diverged 60~80 million years ago and vary by 40-fold in genome size. Measurements were made using a broadly reactive antibody to rice centromeric histone H3 (CENH3). In species-wide comparisons, we found a clear linear relationship between total centromere size and genome size. Species with large genomes and few chromosomes tend to have the largest centromeres (e.g., rye) while species with small genomes and many chromosomes have the smallest centromeres (e.g., rice). However, within a species, centromere size is surprisingly uniform. We present evidence from three oat-maize addition lines that support this claim, indicating that each of three maize centromeres propagated in oat are not measurably different from each other. In the context of previously published data, our results suggest that the apparent correlation between chromosome and centromere size is incidental to a larger trend that reflects genome size. Centromere size may be determined by a limiting component mechanism similar to that described for Caenorhabditis elegans centrosomes.

  6. A preliminary test of estimating forest site quality using species composition in a southern Appalachian watershed

    Treesearch

    W. Henry McNab; David L. Loftis

    2013-01-01

    Characteristic arborescent communities of mesophytic or xerophytic species have long been recognized as indicative of forest site quality in the Southern Appalachians, where soil moisture availability is the primary environmental variable affecting productivity. But, a workable quantitative system of site classification based on species composition is not available. We...

  7. Fatty acid composition of wild mushroom species of order Agaricales--examination by gas chromatography-mass spectrometry and chemometrics.

    PubMed

    Marekov, Ilko; Momchilova, Svetlana; Grung, Bjørn; Nikolova-Damyanova, Boryana

    2012-12-01

    Applying gas chromatography-mass spectrometry of 4,4-dimethyloxazoline fatty acid derivatives, the fatty acid composition of 15 mushroom species belonging to 9 genera and 5 families of order Agaricales growing in Bulgaria is determined. The structure of 31 fatty acids (not all present in each species) is unambiguously elucidated, with linoleic, oleic and palmitic acids being the main components (ranging between 70.9% (Marasmius oreades) and 91.2% (Endoptychum agaricoides)). A group of three hexadecenoic positionally isomeric fatty acids, 6-, 9- and 11-16:1, appeared to be characteristic components of the examined species. By applying chemometrics it was possible to show that the fatty acid composition closely reflects the classification of the species. Copyright © 2012 Elsevier B.V. All rights reserved.

  8. Evolutionary Divergences in Root Exudate Composition among Ecologically-Contrasting Helianthus Species

    PubMed Central

    Bowsher, Alan W.; Ali, Rifhat; Harding, Scott A.; Tsai, Chung-Jui; Donovan, Lisa A.

    2016-01-01

    Plant roots exude numerous metabolites into the soil that influence nutrient availability. Although root exudate composition is hypothesized to be under selection in low fertility soils, few studies have tested this hypothesis in a phylogenetic framework. In this study, we examined root exudates of three pairs of Helianthus species chosen as phylogenetically-independent contrasts with respect to native soil nutrient availability. Under controlled environmental conditions, seedlings were grown to the three-leaf-pair stage, then transferred to either high or low nutrient treatments. After five days of nutrient treatments, we used gas chromatography-mass spectrometry for analysis of root exudates, and detected 37 metabolites across species. When compared in the high nutrient treatment, species native to low nutrient soils exhibited overall higher exudation than their sister species native to high nutrient soils in all three species pairs, providing support for repeated evolutionary shifts in response to native soil fertility. Species native to low nutrient soils and those native to high nutrient soils responded similarly to low nutrient treatments with increased exudation of organic acids (fumaric, citric, malic acids) and glucose, potentially as a mechanism to enhance nutrition acquisition. However, species native to low nutrient soils also responded to low nutrient treatments with a larger decrease in exudation of amino acids than species native to high nutrient soils in all three species pairs. This indicates that species native to low nutrient soils have evolved a unique sensitivity to changes in nutrient availability for some, but not all, root exudates. Overall, these repeated evolutionary divergences between species native to low nutrient soils and those native to high nutrient soils provide evidence for the adaptive value of root exudation, and its plasticity, in contrasting soil environments. PMID:26824236

  9. Evolutionary Divergences in Root Exudate Composition among Ecologically-Contrasting Helianthus Species.

    PubMed

    Bowsher, Alan W; Ali, Rifhat; Harding, Scott A; Tsai, Chung-Jui; Donovan, Lisa A

    2016-01-01

    Plant roots exude numerous metabolites into the soil that influence nutrient availability. Although root exudate composition is hypothesized to be under selection in low fertility soils, few studies have tested this hypothesis in a phylogenetic framework. In this study, we examined root exudates of three pairs of Helianthus species chosen as phylogenetically-independent contrasts with respect to native soil nutrient availability. Under controlled environmental conditions, seedlings were grown to the three-leaf-pair stage, then transferred to either high or low nutrient treatments. After five days of nutrient treatments, we used gas chromatography-mass spectrometry for analysis of root exudates, and detected 37 metabolites across species. When compared in the high nutrient treatment, species native to low nutrient soils exhibited overall higher exudation than their sister species native to high nutrient soils in all three species pairs, providing support for repeated evolutionary shifts in response to native soil fertility. Species native to low nutrient soils and those native to high nutrient soils responded similarly to low nutrient treatments with increased exudation of organic acids (fumaric, citric, malic acids) and glucose, potentially as a mechanism to enhance nutrition acquisition. However, species native to low nutrient soils also responded to low nutrient treatments with a larger decrease in exudation of amino acids than species native to high nutrient soils in all three species pairs. This indicates that species native to low nutrient soils have evolved a unique sensitivity to changes in nutrient availability for some, but not all, root exudates. Overall, these repeated evolutionary divergences between species native to low nutrient soils and those native to high nutrient soils provide evidence for the adaptive value of root exudation, and its plasticity, in contrasting soil environments.

  10. The Shark Assemblage at French Frigate Shoals Atoll, Hawai‘i: Species Composition, Abundance and Habitat Use

    PubMed Central

    Dale, Jonathan J.; Stankus, Austin M.; Burns, Michael S.; Meyer, Carl G.

    2011-01-01

    Empirical data on the abundance and habitat preferences of coral reef top predators are needed to evaluate their ecological impacts and guide management decisions. We used longline surveys to quantify the shark assemblage at French Frigate Shoals (FFS) atoll from May to August 2009. Fishing effort consisted of 189 longline sets totaling 6,862 hook hours of soak time. A total of 221 sharks from 7 species were captured, among which Galapagos (Carcharhinus galapagensis, 36.2%), gray reef (Carcharhinus amblyrhynchos, 25.8%) and tiger (Galeocerdo cuvier, 20.4%) sharks were numerically dominant. A lack of blacktip reef sharks (Carcharhinus melanopterus) distinguished the FFS shark assemblage from those at many other atolls in the Indo-Pacific. Compared to prior underwater visual survey estimates, longline methods more accurately represented species abundance and composition for the majority of shark species. Sharks were significantly less abundant in the shallow lagoon than adjacent habitats. Recaptures of Galapagos sharks provided the first empirical estimate of population size for any Galapagos shark population. The overall recapture rate was 5.4%. Multiple closed population models were evaluated, with Chao Mh ranking best in model performance and yielding a population estimate of 668 sharks with 95% confidence intervals ranging from 289–1720. Low shark abundance in the shallow lagoon habitats suggests removal of a small number of sharks from the immediate vicinity of lagoonal islets may reduce short-term predation on endangered monk seal (Monachus schauinslandi) pups, but considerable fishing effort would be required to catch even a small number of sharks. Additional data on long-term movements and habitat use of sharks at FFS are required to better assess the likely ecological impacts of shark culling. PMID:21347321

  11. Stimulation of soil nitrification and denitrification by grazing in grasslands: do changes in plant species composition matter?

    PubMed

    Le Roux, X; Bardy, M; Loiseau, P; Louault, F

    2003-11-01

    Stimulation of nitrification and denitrification by long term (from years to decades) grazing has commonly been reported in different grassland ecosystems. However, grazing generally induces important changes in plant species composition, and whether changes in nitrification and denitrification are primarily due to changes in vegetation composition has never been tested. We compared soil nitrification- and denitrification-enzyme activities (NEA and DEA, respectively) between semi-natural grassland sites experiencing intensive (IG) and light (LG) grazing/mowing regimes for 13 years. Mean NEA and DEA (i.e. observed from random soil sampling) were higher in IG than LG sites. The NEA/DEA ratio was higher in IG than LG sites, indicating a higher stimulation of nitrification. Marked changes in plant species composition were observed in response to the grazing/mowing regime. In particular, the specific phytomass volume of Elymus repens was lower in IG than LG sites, whereas the specific volume of Lolium perenne was higher in IG than LG sites. In contrast, the specific volume of Holcus lanatus, Poa trivialis and Arrhenatherum elatius were not significantly different between treatments. Soils sampled beneath grass tussocks of the last three species exhibited higher DEA, NEA and NEA/DEA ratio in IG than LG sites. For a given grazing regime, plant species did not affect significantly soil DEA, NEA and NEA/DEA ratio. The modification of plant species composition is thus not the primary factor driving changes in nitrification and denitrification in semi-natural grassland ecosystems experiencing long term intensive grazing. Factors such as trampling, N returned in animal excreta, and/or modification of N uptake and C exudation by frequently defoliated plants could be responsible for the enhanced microbial activities.

  12. Use of DNA barcoding to reveal species composition of convenience seafood.

    PubMed

    Huxley-Jones, Elizabeth; Shaw, Jennifer L A; Fletcher, Carly; Parnell, Juliette; Watts, Phillip C

    2012-04-01

    Increased education of consumers can be an effective tool for conservation of commercially harvested marine species when product labeling is accurate and allows an informed choice. However, generic labeling (e.g., as white fish or surimi) and mislabeling of seafood prevents this and may erode consumer confidence in seafood product labels in general. We used DNA barcoding to identify the species composition of two types of convenience seafood (i.e., products processed for ease of consumption): fish fingers (long pieces of fish covered with bread crumbs or batter, n = 241) and seafood sticks (long pieces of cooked fish, n = 30). In products labeled as either white fish or surimi, four teleost species were present. Less than 1.5% of fish fingers with species-specific information were mislabeled. Results of other studies show substantially more mislabeling (e.g., >25%) of teleost products, which likely reflects the lower economic gains associated with mislabeling of convenience seafood compared with whole fillets. In addition to species identification, seafood product labels should be required to contain information about, for example, harvesting practices, and our data indicate that consumers can have reasonable confidence in the accuracy of the labels of convenience seafood and thus select brands on the basis of information about current fisheries practice. ©2012 Society for Conservation Biology.

  13. Tree species composition and structure in an old bottomland hardwood forest in south-central Arkansas

    Treesearch

    Brian Roy Lockhart; James M. Guldin; Thomas Foti

    2010-01-01

    Tree species composition and structure was determined for an old bottomland hardwood forest located in the Moro Creek Bottoms Natural Area in south-central Arkansas. Diversity for this forest was high with species richness ranging from 33 for the overstory and sapling strata to 26 for the seedling stratum and Shannon-Weiner values of 2.54 to 1.02 for the overstory and...

  14. [Contents of total anthocyanins and total saponins as well as composition of saponin monomers of Purple and Green Notoginseng Radix et Rhizoma].

    PubMed

    Zhao, Chang-ling; Yang, Sheng-chao; Chen, Zhong-jian; Shen, Yong; Wei, Fu-gang; Wang, Wu; Long, Ting-ju

    2014-10-01

    The contents of total anthocyanins and total saponins as well as the composition of saponin monomers of Purple and Green Notoginseng Radix et Rhizoma were studied to compare the medicinal quality and commercial values. Three-year-old Notoginseng Radix et Rhizoma was selected as the research materials. The contents of total anthocyanins and total saponins were determined by spectrophotometry. The compositions of saponin monomers were monitored by HPLC. The significance of content differences was determined by variance analysis. The contents of total anthocyanins and total saponins of Purple Notoginseng Radix et Rhizomawere about 204.85% and 33.86% higher than those of Green Notoginseng Radix et Rhizoma respectively. The Purple and Green Notoginseng Radix et Rhizoma both contained five saponin monomers whose contents were as follows: ginsenoside Rg1 > ginsenoside Rb1 > notoginsenoside R1 > ginsenoside Rd > ginsenoside Re. The contents of notoginsenoside R1, ginsenoside Rd and ginsenoside Re of Purple Notoginseng Radix et Rhizoma were about 16.03%, 10.83% and 5.39% higher than those of Green Notoginseng Radix et Rhizoma respectively. However, the contents of ginsenoside Rg1 and ginsenoside Rb1 of Green Notoginseng Radix et Rhizoma were about 0.93% and 3.33% higher than those of Purple Notoginseng Radix et Rhizoma respectively. With respect to Green Notoginseng Radix et Rhizoma, the increase of the total anthocyanins in Purple Notoginseng Radix et Rhizoma reached a significant level, but the increases of total saponins, notoginsenoside R1, ginsenoside Rd and ginsenoside Re and the decreases of ginsenoside Rg1 and ginsenoside Rb1 did not. The total anthocyanins accumulation in Notoginseng Radix et Rhizoma implies the content increases of the total saponins, notoginsenoside R1, ginsenoside Rd and ginsenoside Re, and the slight decreases of ginsenoside Rg1 and ginsenoside Rb1 contents; but the type and relative contents of saponin monomers remain unchanged. The medicinal

  15. Species composition and habitat characterization of mosquito (Diptera: Culicidae) larvae in semi-urban areas of Dhaka, Bangladesh.

    PubMed

    Bashar, Kabirul; Rahman, Md Sayfur; Nodi, Ila Jahan; Howlader, Abdul Jabber

    2016-03-01

    Mosquito larvae are purely aquatic and develop in water bodies, the type of which is more or less specific to each species. Therefore, a study was carried out to identify the habitat characters of different mosquito species along with their species composition in semi-urban area of Dhaka in Bangladesh during the month of May and June 2012. A total of 6088 mosquito larvae belonging to 12 species (Aedes aegypti, Aedes albopictus, Anopheles barbirostris, Anopheles peditaeniatus, Anopheles vagus, Culex gelidus, Culex hutchinsoni, Culex quinquefasciatus, Culex tritaeniorhynchus, Mansonia annulifera, Mansonia uniformis, and Toxorhynchites splendens) under 5 genera were collected from 14 different types of habitats. Culex quinquefsciatus was the dominant (21.7/500 ml) species followed by Cx. tritaeniorhynchus (10.53/500 ml). Dissolved oxygen and chlorophyll a were the preeminent predictors for the abundance of all collected mosquito larvae except Ae. aegypti. Water temperature was positively associated with the breeding of An. vagus (r = 0.421, p = <0.001), An. barbirostris (r = 0.489, p = <0.001) and An. peditaeniatus (r = 0.375, p = <0.001). Water depth, distance from nearest house, emergent plant coverage, and alkalinity were found as the basis of larval abundance. Every Culex species and Tx. splendens (r = 0.359, p = 0.001) were found positively associated with chemical oxygen demand, while Mn. annulifera showed negative association (r = -0.115, p = 0.0297). This study also highlighted that various physicochemical factors affect the presence or abundance of mosquito larvae.

  16. Species composition and habitat characterization of mosquito (Diptera: Culicidae) larvae in semi-urban areas of Dhaka, Bangladesh

    PubMed Central

    Bashar, Kabirul; Rahman, Md. Sayfur; Nodi, Ila Jahan; Howlader, Abdul Jabber

    2016-01-01

    Mosquito larvae are purely aquatic and develop in water bodies, the type of which is more or less specific to each species. Therefore, a study was carried out to identify the habitat characters of different mosquito species along with their species composition in semi-urban area of Dhaka in Bangladesh during the month of May and June 2012. A total of 6088 mosquito larvae belonging to 12 species (Aedes aegypti, Aedes albopictus, Anopheles barbirostris, Anopheles peditaeniatus, Anopheles vagus, Culex gelidus, Culex hutchinsoni, Culex quinquefasciatus, Culex tritaeniorhynchus, Mansonia annulifera, Mansonia uniformis, and Toxorhynchites splendens) under 5 genera were collected from 14 different types of habitats. Culex quinquefsciatus was the dominant (21.7/500 ml) species followed by Cx. tritaeniorhynchus (10.53/500 ml). Dissolved oxygen and chlorophyll a were the preeminent predictors for the abundance of all collected mosquito larvae except Ae. aegypti. Water temperature was positively associated with the breeding of An. vagus (r = 0.421, p = <0.001), An. barbirostris (r = 0.489, p = <0.001) and An. peditaeniatus (r = 0.375, p = <0.001). Water depth, distance from nearest house, emergent plant coverage, and alkalinity were found as the basis of larval abundance. Every Culex species and Tx. splendens (r = 0.359, p = 0.001) were found positively associated with chemical oxygen demand, while Mn. annulifera showed negative association (r = −0.115, p = 0.0297). This study also highlighted that various physicochemical factors affect the presence or abundance of mosquito larvae. PMID:27241953

  17. Functional implications of changes in seagrass species composition in two shallow coastal lagoons

    EPA Science Inventory

    While the consequences of losing seagrass meadows are well known, there is less information on the functional implications of changes in seagrass species composition. In this study, we use data from a long-term monitoring project in shallow lagoons on the Florida Gulf Coast to as...

  18. Coccolithophore responses to environmental variability in the South China Sea: species composition and calcite content

    NASA Astrophysics Data System (ADS)

    Jin, Xiaobo; Liu, Chuanlian; Poulton, Alex J.; Dai, Minhan; Guo, Xianghui

    2016-08-01

    Coccolithophore contributions to the global marine carbon cycle are regulated by the calcite content of their scales (coccoliths) and the relative cellular levels of photosynthesis and calcification rates. All three of these factors vary between coccolithophore species and with response to the growth environment. Here, water samples were collected in the northern basin of the South China Sea (SCS) during summer 2014 in order to examine how environmental variability influenced species composition and cellular levels of calcite content. Average coccolithophore abundance and their calcite concentration in the water column were 11.82 cells mL-1 and 1508.3 pg C mL-1, respectively, during the cruise. Water samples can be divided into three floral groups according to their distinct coccolithophore communities. The vertical structure of the coccolithophore community in the water column was controlled by the trophic conditions, which were regulated by mesoscale eddies across the SCS basin. The evaluation of coccolithophore-based calcite in the surface ocean also showed that three key species in the SCS (Emiliania huxleyi, Gephyrocapsa oceanica, Florisphaera profunda) and other larger, numerically rare species made almost equal contributions to total coccolith-based calcite in the water column. For Emiliania huxleyi biometry measurements, coccolith size positively correlated with nutrients (nitrate, phosphate), and it is suggested that coccolith length is influenced by light and nutrients through the regulation of growth rates. Larger-sized coccoliths were also linked statistically to low pH and calcite saturation states; however, it is not a simple cause and effect relationship, as carbonate chemistry was strongly co-correlated with the other key environmental factors (nutrients, light).

  19. Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.

    PubMed

    Ohba, Seigo; Sumita, Yoshinori; Umebayashi, Mayumi; Yoshimura, Hitoshi; Yoshida, Hisato; Matsuda, Shinpei; Kimura, Hideki; Asahina, Izumi; Sano, Kazuo

    2016-01-01

    The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma. The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone. The mice were sacrificed and the specimens were harvested four weeks after surgery. The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software. The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline. Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline. However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma. The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Ecological and physiological controls of species composition in green macroalgal blooms.

    PubMed

    Nelson, Timothy A; Haberlin, Karalon; Nelson, Amorah V; Ribarich, Heather; Hotchkiss, Ruth; Van Alstyne, Kathryn L; Buckingham, Lee; Simunds, Dejah J; Fredrickson, Kerri

    2008-05-01

    Green macroalgal blooms have substantially altered marine community structure and function, specifically by smothering seagrasses and other primary producers that are critical to commercial fisheries and by creating anoxic conditions in enclosed embayments. Bottom-up factors are viewed as the primary drivers of these blooms, but increasing attention has been paid to biotic controls of species composition. In Washington State, USA, blooms are often dominated by Ulva spp. intertidally and Ulvaria obscura subtidally. Factors that could cause this spatial difference were examined, including competition, grazer preferences, salinity, photoacclimation, nutrient requirements, and responses to nutrient enrichment. Ulva specimens grew faster than Ulvaria in intertidal chambers but not significantly faster in subtidal chambers. Ulva was better able to acclimate to a high-light environment and was more tolerant of low salinity than Ulvaria. Ulvaria had higher tissue N content, chlorophyll, chlorophyll b: chlorophyll a, and protein content than Ulva. These differences suggest that nitrogen availability could affect species composition. A suite of five grazers preferred Ulva to Ulvaria in choice experiments. Thus, bottom-up factors allow Ulva to dominate the intertidal zone while resistance to grazers appears to allow Ulvaria to dominate the subtidal zone. While ulvoid algae are in the same functional-form group, they are not functionally redundant.

  1. Phytoplankton community composition in nearshore coastal waters of Louisiana

    EPA Science Inventory

    Phytoplankton community compositions within near-shore coastal and estuarine waters of Louisiana were characterized by relative abundance, biovolume, and taxonomic identification to genus and species when possible. The range of total nitrogen was 0.5 to 1.3 mg L-1 and total phos...

  2. Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species.

    PubMed

    Coleman-Derr, Devin; Desgarennes, Damaris; Fonseca-Garcia, Citlali; Gross, Stephen; Clingenpeel, Scott; Woyke, Tanja; North, Gretchen; Visel, Axel; Partida-Martinez, Laila P; Tringe, Susannah G

    2016-01-01

    Desert plants are hypothesized to survive the environmental stress inherent to these regions in part thanks to symbioses with microorganisms, and yet these microbial species, the communities they form, and the forces that influence them are poorly understood. Here we report the first comprehensive investigation of the microbial communities associated with species of Agave, which are native to semiarid and arid regions of Central and North America and are emerging as biofuel feedstocks. We examined prokaryotic and fungal communities in the rhizosphere, phyllosphere, leaf and root endosphere, as well as proximal and distal soil samples from cultivated and native agaves, through Illumina amplicon sequencing. Phylogenetic profiling revealed that the composition of prokaryotic communities was primarily determined by the plant compartment, whereas the composition of fungal communities was mainly influenced by the biogeography of the host species. Cultivated A. tequilana exhibited lower levels of prokaryotic diversity compared with native agaves, although no differences in microbial diversity were found in the endosphere. Agaves shared core prokaryotic and fungal taxa known to promote plant growth and confer tolerance to abiotic stress, which suggests common principles underpinning Agave-microbe interactions. No claim to US Government works. New Phytologist © 2015 New Phytologist Trust.

  3. Species-specific responses to climate change and community composition determine future calcification rates of Florida Keys reefs.

    PubMed

    Okazaki, Remy R; Towle, Erica K; van Hooidonk, Ruben; Mor, Carolina; Winter, Rivah N; Piggot, Alan M; Cunning, Ross; Baker, Andrew C; Klaus, James S; Swart, Peter K; Langdon, Chris

    2017-03-01

    Anthropogenic climate change compromises reef growth as a result of increasing temperatures and ocean acidification. Scleractinian corals vary in their sensitivity to these variables, suggesting species composition will influence how reef communities respond to future climate change. Because data are lacking for many species, most studies that model future reef growth rely on uniform scleractinian calcification sensitivities to temperature and ocean acidification. To address this knowledge gap, calcification of twelve common and understudied Caribbean coral species was measured for two months under crossed temperatures (27, 30.3 °C) and CO 2 partial pressures (pCO 2 ) (400, 900, 1300 μatm). Mixed-effects models of calcification for each species were then used to project community-level scleractinian calcification using Florida Keys reef composition data and IPCC AR5 ensemble climate model data. Three of the four most abundant species, Orbicella faveolata, Montastraea cavernosa, and Porites astreoides, had negative calcification responses to both elevated temperature and pCO 2 . In the business-as-usual CO 2 emissions scenario, reefs with high abundances of these species had projected end-of-century declines in scleractinian calcification of >50% relative to present-day rates. Siderastrea siderea, the other most common species, was insensitive to both temperature and pCO 2 within the levels tested here. Reefs dominated by this species had the most stable end-of-century growth. Under more optimistic scenarios of reduced CO 2 emissions, calcification rates throughout the Florida Keys declined <20% by 2100. Under the most extreme emissions scenario, projected declines were highly variable among reefs, ranging 10-100%. Without considering bleaching, reef growth will likely decline on most reefs, especially where resistant species like S. siderea are not already dominant. This study demonstrates how species composition influences reef community responses to climate

  4. Cosmic ray interactions with lunar materials - Nature and composition of species formed

    NASA Technical Reports Server (NTRS)

    Mukherjee, N. R.

    1976-01-01

    The paper discusses the effect of cosmic-ray proton interactions with lunar material, the nature and composition of the species resulting from these interactions, and the contribution of these species to the lunar atmosphere. It is shown that hydrogen atoms resulting from cosmic-ray proton neutralization escape into the atmosphere mostly as H2, that only a small fraction of the very small amount of OH and H2O produced by cosmic-ray protons escapes into the atmosphere, and that cosmic-ray protons play a very minor role, as compared with solar-wind protons, in producing lunar atmospheric hydrogen and hydrogenated species. It is concluded that the atmospheric contributions of H2, H, OH, and H2O produced by cosmic-ray protons are about three orders of magnitude less than those due to solar-wind protons.

  5. An imputed forest composition map for New England screened by species range boundaries

    Treesearch

    Matthew J. Duveneck; Jonathan R. Thompson; B. Tyler Wilson

    2015-01-01

    Initializing forest landscape models (FLMs) to simulate changes in tree species composition requires accurate fine-scale forest attribute information mapped continuously over large areas. Nearest-neighbor imputation maps, maps developed from multivariate imputation of field plots, have high potential for use as the initial condition within FLMs, but the tendency for...

  6. Climate and soil texture influence patterns of forb species richness and composition in big sagebrush plant communities across their spatial extent in the western US

    USGS Publications Warehouse

    Pennington, Victoria E.; Palmquist, Kyle A.; Bradford, John B.; Lauenroth, William K.

    2017-01-01

    Article for outlet: Plant Ecology. Abstract: Big sagebrush (Artemisia tridentata Nutt.) plant communities are widespread non-forested drylands in western North American and similar to all shrub steppe ecosystems world-wide are composed of a shrub overstory layer and a forb and graminoid understory layer. Forbs account for the majority of plant species diversity in big sagebrush plant communities and are important for ecosystem function. Few studies have explored the geographic patterns of forb species richness and composition and their relationships with environmental variables in these communities. Our objectives were to examine the small and large-scale spatial patterns in forb species richness and composition and the influence of environmental variables. We sampled forb species richness and composition along transects at 15 field sites in Colorado, Idaho, Montana, Nevada, Oregon, Utah, and Wyoming, built species-area relationships to quantify differences in forb species richness at sites, and used Principal Components Analysis and nonmetric multidimensional scaling to identify relationships among environmental variables and forb species richness and composition. We found that species richness was most strongly correlated with soil texture, while species composition was most related to climate. The combination of climate and soil texture influences water availability, with important consequences for forb species richness and composition, which suggests climate-change induced modification of soil water availability may have important implications for plant species diversity in the future. Our paper is the first to our knowledge to examine forb biodiversity patterns in big sagebrush ecosystems in relation to environmental factors across the big sagebrush region.

  7. Comparative study of lipids in mature seeds of six Cordia species (family Boraginaceae) collected in different regions of Brazil.

    PubMed

    Carvalho, Patricía de O; Arrebola, Melissa B; Sawaya, Alexandra C H F; Cunha, Ildenize B S; Bastos, Deborah H M; Eberlin, Marcos N

    2006-08-01

    The oil content, FA, and lipid class composition of the mature seeds of six Cordia species were analyzed. Mature seeds of each species were collected in their natural habitat from 2002 to 2004. The total lipid content varied from 1.9% to 13.2%, there being significant differences between the results found in different years for each species and between the species analyzed. The contents of FFA varied from 2.0% to 7.9% of total lipids. Neutral lipids (NL) were the largest class, making up between 89.6% and 96.4% of the total lipids; the phospholipids (PL) were the second largest class (3.0% to 8.9% of the total lipids), and the glycolipids (GL) were the smallest class (0.6 to 3.4%). The presence of GLA was determined in each class of lipids; it is predominant in the NL. Levels of GLA ranged from 1.2% to 6.8% of total seed FA. This is, to our knowledge the first study of lipid composition in seeds of species of Cordia from Brazil.

  8. Evaluation of morphological indices and total body electrical conductivity to assess body composition in big brown bats

    USGS Publications Warehouse

    Pearce, R.D.; O'Shea, T.J.; Wunder, B.A.

    2008-01-01

    Bat researchers have used both morphological indices and total body electric conductivity (TOBEC) as proxies for body condition in a variety of studies, but have typically not validated these indices against direct measurement of body composition. We quantified body composition (total carcass lipids) to determine if morphological indices were useful predictors of body condition in big brown bats (Eptesicus fuscus). We also evaluated body composition indirectly by TOBEC using EM-SCAN?? technology. The most important predictors of body composition in multiple regression analysis were body mass-to-forearm ratio (partial r2 = 0.82, P < 0.001) followed by TOBEC measurement (partial r2 = 0.08, P < 0.001) and to a minor extent head length (partial r2 = 0.02, P < 0.05). Morphological condition indices alone may be adequate for some studies because of lower cost and effort. Marking bats with passive integrated transponder (PIT) tags affected TOBEC measurements. ?? Museum and Institute of Zoology PAS.

  9. Metagenomic systems biology and metabolic modeling of the human microbiome: from species composition to community assembly rules.

    PubMed

    Levy, Roie; Borenstein, Elhanan

    2014-01-01

    The human microbiome is a key contributor to health and development. Yet little is known about the ecological forces that are at play in defining the composition of such host-associated communities. Metagenomics-based studies have uncovered clear patterns of community structure but are often incapable of distinguishing alternative structuring paradigms. In a recent study, we integrated metagenomic analysis with a systems biology approach, using a reverse ecology framework to model numerous human microbiota species and to infer metabolic interactions between species. Comparing predicted interactions with species composition data revealed that the assembly of the human microbiome is dominated at the community level by habitat filtering. Furthermore, we demonstrated that this habitat filtering cannot be accounted for by known host phenotypes or by the metabolic versatility of the various species. Here we provide a summary of our findings and offer a brief perspective on related studies and on future approaches utilizing this metagenomic systems biology framework.

  10. Species Composition and Distribution of Adult Anopheles (Diptera: Culicidae) in Panama

    PubMed Central

    LOAIZA, J. R.; BERMINGHAM, E.; SCOTT, M. E.; ROVIRA, J. R.; CONN, J. E.

    2010-01-01

    Anopheles (Diptera: Culicidae) species composition and distribution were studied using human landing catch data over a 35-yr period in Panama. Mosquitoes were collected from 77 sites during 228 field trips carried out by members of the National Malaria Eradication Service. Fourteen Anopheles species were identified. The highest average human biting rates were recorded from Anopheles (Nyssorhynchus) albimanus (Wiedemann) (9.8 bites/person/night) and Anopheles (Anopheles) punctimacula (Dyar and Knab) (6.2 bites/person/night). These two species were also the most common, present in 99.1 and 74.9%, respectively, of the sites. Anopheles (Nyssorhynchus) aquasalis (Curry) was encountered mostly in the indigenous Kuna Yala Comarca along the eastern Atlantic coast, where malaria case history and average human biting rate (9.3 bites/person/night) suggest a local role in malaria transmission. An. albimanus, An. punctimacula, and Anopheles (Anopheles) vestitipennis (Dyar and Knab) were more abundant during the rainy season (May–December), whereas An. aquasalis was more abundant in the dry season (January–April). Other vector species collected in this study were Anopheles (Kerteszia) neivai (Howard, Dyar, and Knab) and Anopheles (Anopheles) pseudopunctipennis s.l. (Theobald). High diversity of Anopheles species and six confirmed malaria vectors in endemic areas of Panama emphasize the need for more detailed studies to better understand malaria transmission dynamics. PMID:18826025

  11. Plant biomass and species composition along an environmental gradient in montane riparian meadows

    Treesearch

    Kathleen A. Dwire; J. Boone Kauffman; E. N. Jack Brookshire; John E. Baham

    2004-01-01

    In riparian meadows, narrow zonation of the dominant vegetation frequently occurs along the elevational gradient from the stream edge to the floodplain terrace. We measured plant species composition and above- and belowground biomass in three riparian plant communities - a priori defined as wet, moist, and dry meadow - along short streamside topographic gradients in...

  12. [Evaluation of the total biological activity and allergenic composition of allergenic extracts].

    PubMed

    Lombardero, M; González, R; Duffort, O; Juan, F; Ayuso, R; Ventas, P; Cortés, C; Carreira, J

    1986-01-01

    In the present study, a complete procedure is presented in order to standardize allergenic extracts, the meaning of which is the measurement of the total allergenic activity and the determination of the allergenic composition. The measurement of the biological activity comprises 2 steps: Preparation of Reference Extracts and determination of their "in vivo" activity. Evaluation of the total allergenic activity of extracts for clinical use. Reference extracts were prepared from the main allergens and their "in vivo" biological activity was determined by a quantitative skin prick test in a sample of at least 30 allergic patients. By definition, the protein concentration of Reference Extract that produces, in the allergic population, a geometric mean wheal of 75 mm.2 has an activity of 100 biological units (BUs). The determination of the biological activity of a problem extract is made by RAST inhibition. The sample is compared with the corresponding Reference Extract by this technique and, from this comparison, it is possible to quantify the activity of the problem extract in biologic units (BUs) with clinical significance. Likewise, different techniques have been used to determine the allergenic composition of extracts. These techniques comprise 2 steps: Separation of the components of the extract. Identification of the components that bind specific human IgE. The separation of the components of the extract has been carried out by isoelectric focusing (IEF) and electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE). In order to identify the allergenic components, an immunoblotting technique has been employed. The separated components in the IEF gel or SDS-PAGE gel are transferred to a nitrocellulose sheet and later on, this membrane is overlaid with a serum pool from allergic patients and a mouse monoclonal anti-human IgE, labelled with 125I. Finally, the autoradiography of the nitrocellulose membrane is obtained. In this way it is possible to compare

  13. Fatty acid composition of bacteria associated with the toxic dinoflagellate Ostreopsis lenticularis and with Caribbean Palythoa species.

    PubMed

    Carballeira, N M; Emiliano, A; Sostre, A; Restituyo, J A; González, I M; Colón, G M; Tosteson, C G; Tosteson, T R

    1998-06-01

    The fatty acid composition of a Pseudomonas sp. (Alteromonas) and its host, the dinoflagellate Ostreopsis lenticularis, vectors in ciguatera fish poisoning, has been studied. The major fatty acids in O. lenticularis were 16:0, 20:5n-3, and 22:6n-3, but 18:2n-6, 18:3n-3, and 18:n-3 were also identified. In contrast to other dinoflagellates, 1 8:5n-3 was not detected in O. lenticularis. Even-chain fatty acids such as 9-16:1, 11-18:1, and 13-20:1 predominated in the Pseudomonas sp. from O. lenticularis, but 1 6-20% of (E)-11-methyl-12-octadecenoic acid was also identified. The chirality of the latter was confirmed by total synthesis (28% overall yield) starting from oxacyclotridecan-2-one. The fatty acid compositions of two other Pseudomonas species, from the palytoxin-producing zoanthids Palythoa mamillosa and P. caribdea, were also studied and were similar to that of the Pseudomonas sp. from O. lenticularis. The possibility of using some of these fatty acids as chemotaxonomic lipids in identifying marine animals that consume toxic dinoflagellates or zoanthids is discussed.

  14. [Impacts of human disturbance on the species composition of higher plants in the wetlands around Dianchi Lake, Yunnan Province of Southwest China].

    PubMed

    Xiang, Xi-Xi; Wu, Zhao-Lu; Luo, Kang; Ding, Hong-Bo; Zhang, Hai-Yan

    2013-09-01

    Introducing higher plants to build semi-natural wetland ecosystem is one of the key approaches to restore the wetlands and lakes that suffered from serious pollution and destruction. Based on the investigation data from 128 quadrats at 26 sampling sites in the wetlands around Dianchi Lake in December 2011-October 2012, and in combining with the references published in the 1960s, this paper discussed the impacts of human activities on the species composition of higher plants in the wetlands around the Lake. In 2012, there were 299 species of 88 families in the wetlands, of which, 181 species were native species, and 118 species were alien ones (including 32 invasive species). Of the 42 species of hydrophytes in the total species, 13 species were alien ones (including 2 invasive species). In comparing with the species data recorded in the 1960s, 232 plants were newly recorded and 43 species disappeared in 2012. Aquatic plants changed obviously. The decreased species were 2 submerged plants, 2 floating plants, and 5 floating leaved plants, and the increased species were 8 emergent plants. Fourteen community types were identified by cluster analysis, of which, the main communities were those dominated by alien species including Pistia stratiotes and Alternanthera philoxeroides. As compared with the data in the 1960s, the plant communities dominated by native species such as Ottelia acuminate and Vallisneria natans were not found presently. Therefore, in the practice of introducing higher plants to restore the degraded wetlands and lakes, it would be necessary to scientifically and appropriately select and blend plant species to avoid the wetland degradation by human activities.

  15. Total selenium and selenium species in irrigation drain inflows to the Salton Sea, California, April and July 2007

    USGS Publications Warehouse

    May, Thomas W.; Walther, Michael J.; Saiki, Michael K.; Brumbaugh, William G.

    2007-01-01

    This report presents the results for two sampling periods during a 4-year monitoring survey to provide a characterization of selenium concentrations in selected irrigation drains flowing into the Salton Sea, California. Total selenium, selenium species, and total suspended solids were determined in water samples, and total selenium was determined in sediment, detritus, and biota that included algae, plankton, midge larvae (family, Chironomidae), and two fish species-western mosquitofish (Gambusia affinis), and sailfin molly (Poecilia latipinna). In addition, sediments were analyzed for percent total organic carbon and particle size. Total selenium concentrations in water for both sampling periods ranged from 1.43 to 47.1 micrograms per liter, predominately as selenate, which is typical of waters leached out of selenium-contaminated marine shales under alkaline and oxidizing conditions. Total selenium concentrations ranged from 0.88 to 20.2 micrograms per gram in biota, and from 0.15 to 28.9 micrograms per gram in detritus and sediment.

  16. Size of clearcut opening affects species composition, growth rate, and stand characteristics.

    Treesearch

    Martin E. Dale; H. Clay Smith; Jeffrey N. Pearcy

    1995-01-01

    In the late 1950's and early 1960's, a series of studies was installed in the central hardwood forest to determine if size of clearcut opening affects the growth rate and species composition of new stands. In 1991, about 30 years after cutting, stand data were collected in 89 openings ranging in size from 0.04 to 1.61 acres. The number of stems per acre...

  17. Annual cycle of zooplankton abundance and species composition in Izmit Bay (the northeastern Marmara Sea)

    NASA Astrophysics Data System (ADS)

    Isinibilir, Melek; Kideys, Ahmet E.; Tarkan, Ahmet N.; Yilmaz, I. Noyan

    2008-07-01

    The monthly abundance, biomass and taxonomic composition of zooplankton of Izmit Bay (the northeastern Marmara Sea) were studied from October 2001 to September 2002. Most species within the zooplankton community displayed a clear pattern of succession throughout the year. Generally copepods and cladocerans were the most abundant groups, while the contribution of meroplankton increased at inner-most stations and dominated the zooplankton. Both species number ( S) and diversity ( H') were positively influenced by the increase in salinity of upper layers ( r = 0.30 and r = 0.31, p < 0.001, respectively), while chlorophyll a was negatively affected ( r = -0.36, p < 0.001). Even though Noctiluca scintillans had a significant seasonality ( F11,120 = 8.45, p < 0.001, ANOVA), abundance was not related to fluctuations in temperature and only chlorophyll a was adversely correlated ( r = -0.35, p < 0.001). In general, there are some minor differences in zooplankton assemblages of upper and lower layers. A comparison of the species composition and abundance of Izmit Bay with other Black Sea bays reveals a high similarity between them.

  18. Species composition, abundance, and seasonal dynamics of stink bugs (Hemiptera: Pentatomidae) in Minnesota soybean fields.

    PubMed

    Koch, Robert L; Pahs, Tiffany

    2014-08-01

    Stink bugs (Hemiptera: Pentatomidae) have historically not been pests of soybean in Minnesota. In response to the invasion of Halyomorpha halys (Stål) and reports of increasing abundance of species native to North America, a state-wide survey of soybean was conducted over 3 yr in Minnesota to determine species composition, abundance, and seasonal dynamics of Pentatomidae associated with soybean. Fourteen species of Pentatomidae (12 herbivorous and two predatory) were collected from soybean. H. halys was not detected in this survey. Among the herbivorous species found, adults of Euschistus variolarius (Palisot de Beauvois) had the greatest relative abundance (60.51%) and frequency of detection (18.44%), followed by Euschistus servus euschistoides (Say) (19.37 and 3.04%, respectively) and Chinavia hilaris (Say) (5.50 and 1.69%, respectively). Abundance of herbivorous nymphs and adults exceeded an economic threshold (20 nymphs and adults per 100 sweeps) in 0.82% of fields in 2012 but not in 2011 or 2013. The frequency of detection of herbivorous species and ratio of nymphs to adults increased with increasing reproductive growth stage of soybean. In two of three years, herbivorous adults were more abundant in the edge compared with interior of fields. Two predatory Pentatomidae, Podisus maculiventris (Say) and Podisus placidus Uhler, comprised 5.95 and 1.62% of the pentatomid adults. Though the species composition of Pentatomidae in Minnesota soybean differs from that in eastern and southern states, the spatial (i.e., greater abundance near field edge) and seasonal dynamics (i.e., increasing abundance and reproduction with increasing reproductive maturity of soybean) in soybean appear similar.

  19. Substantial equivalence analysis in fruits from three Theobroma species through chemical composition and protein profiling.

    PubMed

    Pérez-Mora, Walter; Jorrin-Novo, Jesús V; Melgarejo, Luz Marina

    2018-02-01

    Substantial equivalence studies were performed in three Theobroma spp., cacao, bicolor and grandiflorum through chemical composition analysis and protein profiling of fruit (pulp juice and seeds). Principal component analysis of sugar, organic acid, and phenol content in pulp juice revealed equivalence among the three species, with differences in some of the compounds that may result in different organoleptic properties. Proteins were extracted from seeds and pulp juice, resolved by two dimensional electrophoresis and major spots subjected to mass spectrometry analysis and identification. The protein profile, as revealed by principal component analysis, was variable among the three species in both seed and pulp, with qualitative and quantitative differences in some of protein species. The functional grouping of the identified proteins correlated with the biological role of each organ. Some of the identified proteins are of interest, being minimally discussed, including vicilin, a protease inhibitor, and a flavonol synthase/flavanone 3-hydroxylase. Theobroma grandiflorum and Theobroma bicolor are endemic Amazonian plants that are poorly traded at the local level. As close relatives of Theobroma cacao, they may provide a good alternative for human consumption and industrial purposes. In this regard, we performed equivalence studies by conducting a comparative biochemical and proteomics analysis of the fruit, pulp juice and seeds of these three species. The results indicated equivalent chemical compositions and variable protein profiles with some differences in the content of the specific compounds or protein species that may result in variable organoleptic properties between the species and can be exploited for traceability purposes. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Species Composition and Distribution of Zooplankton from Northeastern Sakhalin Shelf (Sea of Okhotsk)

    NASA Astrophysics Data System (ADS)

    Kasyan, V. V.

    2018-03-01

    The species composition, density, biomass, and distribution of zooplankton of the northeastern Sakhalin shelf, Sea of Okhotsk (Chaivo, Pil'tunskii, and Morskoi regions) were studied in October 2014. Zooplankton was represented by 15 taxonomic groups, which were dominated by Copepoda (13 species). The average density and biomass was highest in the Chaivo region (14112 ± 4322 ind./m3, 395 ± 107 mg/m3) and in the Pil'tunskii region (16692 ± 10707 ind./m3, 346 ± 233 mg/m3); the abundance of detected taxonomic groups was minimal (8-12). The average density and biomass of zooplankton was up to 4304 ± 2441 ind./m3, 133 ± 77 mg/m3 in the Morskoi region and increased with depth; the abundance of taxa was maximum (15). Four species of copepods made up the majority of the density and biomass of zooplankton: Acartia hudsonica, Eurytemora herdmani, Pseudocalanus newmani, and Oithona similis. In the Chaivo region, species of the genera Acartia, Eurytemora, and Oithona dominated and subdominated; in Pil'tunskii region, species of the genera Acartia and Oithona dominated and subdominated; and in the Morskoi region, species of the genera Oithona, Pseudocalanus, and Acartia dominated and subdominated.

  1. Intercropping With Fruit Trees Increases Population Abundance and Alters Species Composition of Spider Mites on Cotton.

    PubMed

    Li, Haiqiang; Pan, Hongsheng; Wang, Dongmei; Liu, Bing; Liu, Jian; Zhang, Jianping; Lu, Yanhui

    2018-05-05

    With the recent increase in planting of fruit trees in southern Xinjiang, the intercropping of fruit trees and cotton has been widely adopted. From 2014 to 2016, a large-scale study was conducted in Aksu, an important agricultural area in southern Xinjiang, to compare the abundance and species composition of spider mites in cotton fields under jujube-cotton, apple-cotton, and cotton monocrop systems. The abundance of spider mites in cotton fields under both intercropping systems was generally higher than in the cotton monocrop. The species composition of spider mites also differed greatly between cotton intercropped with apple or jujube compared to the cotton monocrop. The relative proportion of Tetranychus truncates Ehara (Acari: Tetranychidae) in the species complex generally increased while that of another spider mite, Tetranychus dunhuangensis Wang (Acari: Tetranychidae), decreased under fruit tree-cotton systems. More attention should be paid to the monitoring and management of spider mites, especially T. truncates in this important region of China.

  2. The Influence of Exotic Lady Beetle (Coleoptera: Coccinellidae) Establishment on the Species Composition of the Native Lady Beetle Community in Missouri.

    PubMed

    Diepenbrock, Lauren M; Fothergill, Kent; Tindall, Kelly V; Losey, John E; Smyth, Rebecca R; Finke, Deborah L

    2016-08-01

    The diversity and abundance of native lady beetles (Coccinellidae) in North America has declined in recent decades. This decline is often correlated with the introduction and establishment of exotic lady beetle species, including Coccinella septempunctata L. and Harmonia axyridis Pallas, suggesting that exotic species precipitated the decline of native lady beetles. We examined species records of native coccinellids in Missouri over 118 yr and asked whether the species composition of the community experienced a shift following the establishment of the exotic species. We found that the contemporary native coccinellid community is different from the community that was present nearly a century ago. However, there was no evidence for a recent abrupt shift in composition triggered by the establishment of exotic species. Instead, our data suggest that the native lady beetle community has been undergoing consistent and gradual change over time, with some species decreasing in abundance and others increasing. While not excluding exotic species as a factor contributing to the decline of native lady beetle species, our findings suggest that other continuous factors, like land use change, may have played a more influential role in determining the composition of the native coccinellid communities within our region. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  3. Species composition and seasonal abundance of stink bugs (Hemiptera: Pentatomidae) in Louisiana soybean.

    PubMed

    Temple, J H; Davis, J A; Micinski, S; Hardke, J T; Price, P; Leonard, B R

    2013-08-01

    In Louisiana during the last decade, the redbanded stink bug, Piezodorus guildinii (Westwood), has become a significant and yield-limiting pest of soybean. The redbanded stink bug was previously reported in the United States in 1892, but was never considered an economically important pest until recently. Soybeans representing four maturity groups (MG) III, IV, V, and VI were sampled weekly from beginning bloom (R1) to physiological maturity (R8) during 2008-2010 at five locations across Louisiana to determine the Pentatomidae composition. In total, 13,146 stink bugs were captured and subsequently identified to species. The predominant species included the redbanded stink bug (54.2%); southern green stink bug (27.1%), Nezara viridula L.; brown stink bug (6.6%), Euschistus servus (Say); and green stink bug (5.5%), Acrosternum hilare (Say). Redbanded stink bug comprised the largest percentage of the complex collected at four of the five survey sites. Numbers exceeding action thresholds of this stink bug complex were only detected during R4 to R7 growth stages. Redbanded stink bug accounted for the largest percentage of the stink bug complex in early maturing soybean varieties (MG III [86%] and IV [60%]) and declined in later maturing soybeans (MG V [54%] and VI [50%]). The redbanded stink bug was initially identified in southern Louisiana during 2000 and had been reported in all soybean producing regions in Louisiana by 2006. This survey is the first to report the redbanded stink bug as a predominant pest of soybeans from locations within the United States.

  4. Assessment of the yeast species composition of cocoa bean fermentations in different cocoa-producing regions using denaturing gradient gel electrophoresis.

    PubMed

    Papalexandratou, Zoi; De Vuyst, Luc

    2011-11-01

    The yeast species composition of 12 cocoa bean fermentations carried out in Brazil, Ecuador, Ivory Coast and Malaysia was investigated culture-independently. Denaturing gradient gel electrophoresis of 26S rRNA gene fragments, obtained through polymerase chain reaction with universal eukaryotic primers, was carried out with two different commercial apparatus (the DCode and CBS systems). In general, this molecular method allowed a rapid monitoring of the yeast species prevailing during fermentation. Under similar and optimal denaturing gradient gel electrophoresis conditions, the CBS system allowed a better separated band pattern than the DCode system and an unambiguous detection of the prevailing species present in the fermentation samples. The most frequent yeast species were Hanseniaspora sp., followed by Pichia kudriavzevii and Saccharomyces cerevisiae, independent of the origin of the cocoa. This indicates a restricted yeast species composition of the cocoa bean fermentation process. Exceptionally, the Ivorian cocoa bean box fermentation samples showed a wider yeast species composition, with Hyphopichia burtonii and Meyerozyma caribbica among the main representatives. Yeasts were not detected in the samples when the temperature inside the fermenting cocoa pulp-bean mass reached values higher than 45 °C or under early acetic acid production conditions. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  5. Fish composition and species richness in eastern South American coastal lagoons: additional support for the freshwater ecoregions of the world.

    PubMed

    Petry, A C; Guimarães, T F R; Vasconcellos, F M; Hartz, S M; Becker, F G; Rosa, R S; Goyenola, G; Caramaschi, E P; Díaz de Astarloa, J M; Sarmento-Soares, L M; Vieira, J P; Garcia, A M; Teixeira de Mello, F; de Melo, F A G; Meerhoff, M; Attayde, J L; Menezes, R F; Mazzeo, N; Di Dario, F

    2016-07-01

    The relationships between fish composition, connectivity and morphometry of 103 lagoons in nine freshwater ecoregions (FEOW) between 2·83° S and 37·64° S were evaluated in order to detect possible congruence between the gradient of species richness and similarities of assemblage composition. Most lagoons included in the study were <2 km(2) , with a maximum of 3975 km(2) in surface area. Combined surface area of all lagoons included in the study was 5411 km(2) . Number of species varied locally from one to 76. A multiple regression revealed that latitude, attributes of morphometry and connectivity, and sampling effort explained a large amount of variability in species richness. Lagoon area was a good predictor of species richness except in low latitude ecoregions, where lagoons are typically small-sized and not affected by marine immigrants, and where non-native fish species accounted for a significant portion of species richness. Relationships between species and area in small-sized lagoons (<2 km(2) ) is highly similar to the expected number in each ecoregion, with systems located between 18·27° S and 30·15° S attaining higher levels of species richness. Similarities in species composition within the primary, secondary and peripheral or marine divisions revealed strong continental biogeographic patterns only for species less tolerant or intolerant to salinity. Further support for the FEOW scheme in the eastern border of South America is therefore provided, and now includes ecotonal systems inhabited simultaneously by freshwater and marine species of fishes. © 2016 The Fisheries Society of the British Isles.

  6. Impact of cultivation on characterisation of species composition of soil bacterial communities.

    PubMed

    McCaig, A E.; Grayston, S J.; Prosser, J I.; Glover, L A.

    2001-03-01

    The species composition of culturable bacteria in Scottish grassland soils was investigated using a combination of Biolog and 16S rDNA analysis for characterisation of isolates. The inclusion of a molecular approach allowed direct comparison of sequences from culturable bacteria with sequences obtained during analysis of DNA extracted directly from the same soil samples. Bacterial strains were isolated on Pseudomonas isolation agar (PIA), a selective medium, and on tryptone soya agar (TSA), a general laboratory medium. In total, 12 and 21 morphologically different bacterial cultures were isolated on PIA and TSA, respectively. Biolog and sequencing placed PIA isolates in the same taxonomic groups, the majority of cultures belonging to the Pseudomonas (sensu stricto) group. However, analysis of 16S rDNA sequences proved more efficient than Biolog for characterising TSA isolates due to limitations of the Microlog database for identifying environmental bacteria. In general, 16S rDNA sequences from TSA isolates showed high similarities to cultured species represented in sequence databases, although TSA-8 showed only 92.5% similarity to the nearest relative, Bacillus insolitus. In general, there was very little overlap between the culturable and uncultured bacterial communities, although two sequences, PIA-2 and TSA-13, showed >99% similarity to soil clones. A cloning step was included prior to sequence analysis of two isolates, TSA-5 and TSA-14, and analysis of several clones confirmed that these cultures comprised at least four and three sequence types, respectively. All isolate clones were most closely related to uncultured bacteria, with clone TSA-5.1 showing 99.8% similarity to a sequence amplified directly from the same soil sample. Interestingly, one clone, TSA-5.4, clustered within a novel group comprising only uncultured sequences. This group, which is associated with the novel, deep-branching Acidobacterium capsulatum lineage, also included clones isolated

  7. Species Composition and Seasonal Distribution of Mosquito Larvae (Diptera: Culicidae) in Southern New Jersey, Burlington County.

    PubMed

    Verna, Thomas N

    2015-09-01

    A total of 36,495 larvae consisting of 45 species from 11 genera were collected from 7,189 sites from southern New Jersey, Burlington County between the months of March and October, 2001-2014. Density and seasonal distribution were determined among natural and artificial habitat. The most dominant species collected from natural habitat was Aedes vexans (Meigen) followed by Ochlerotatus canadensis canadensis (Theobald), Culex restuans Theobald, Culex pipiens L., and Culex territans Walker. The most dominant species collected from artificial habitat was Aedes albopictus (Skuse) followed by Ochlerotatus japonicus japonicus (Theobald), Cx. restuans, Cx. pipiens, and Ochlerotatus triseriatus (Say). Cx. restuans and Cx. pipiens were the only species categorized as dominant among both natural and artificial habitat and comprised greater than half the total density. Sympatry was common among dominant species from artificial habitat where a significant percentage of the total collection contained multiple species. The most common types of natural habitats were forested depressions and stream flood plains whereas rimless vehicle tires and various plastic containers were the most common artificial habitats. The pitcher plant Sarracenia purpurea L. was the only habitat exclusive to one species. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  8. The costs of evaluating species densities and composition of snakes to assess development impacts in amazonia.

    PubMed

    Fraga, Rafael de; Stow, Adam J; Magnusson, William E; Lima, Albertina P

    2014-01-01

    Studies leading to decision-making for environmental licensing often fail to provide accurate estimates of diversity. Measures of snake diversity are regularly obtained to assess development impacts in the rainforests of the Amazon Basin, but this taxonomic group may be subject to poor detection probabilities. Recently, the Brazilian government tried to standardize sampling designs by the implementation of a system (RAPELD) to quantify biological diversity using spatially-standardized sampling units. Consistency in sampling design allows the detection probabilities to be compared among taxa, and sampling effort and associated cost to be evaluated. The cost effectiveness of detecting snakes has received no attention in Amazonia. Here we tested the effects of reducing sampling effort on estimates of species densities and assemblage composition. We identified snakes in seven plot systems, each standardised with 14 plots. The 250 m long centre line of each plot followed an altitudinal contour. Surveys were repeated four times in each plot and detection probabilities were estimated for the 41 species encountered. Reducing the number of observations, or the size of the sampling modules, caused significant loss of information on species densities and local patterns of variation in assemblage composition. We estimated the cost to find a snake as $ 120 U.S., but general linear models indicated the possibility of identifying differences in assemblage composition for half the overall survey costs. Decisions to reduce sampling effort depend on the importance of lost information to target-issues, and may not be the preferred option if there is the potential for identifying individual snake species requiring specific conservation actions. However, in most studies of human disturbance on species assemblages, it is likely to be more cost-effective to focus on other groups of organisms with higher detection probabilities.

  9. The Costs of Evaluating Species Densities and Composition of Snakes to Assess Development Impacts in Amazonia

    PubMed Central

    de Fraga, Rafael; Stow, Adam J.; Magnusson, William E.; Lima, Albertina P.

    2014-01-01

    Studies leading to decision-making for environmental licensing often fail to provide accurate estimates of diversity. Measures of snake diversity are regularly obtained to assess development impacts in the rainforests of the Amazon Basin, but this taxonomic group may be subject to poor detection probabilities. Recently, the Brazilian government tried to standardize sampling designs by the implementation of a system (RAPELD) to quantify biological diversity using spatially-standardized sampling units. Consistency in sampling design allows the detection probabilities to be compared among taxa, and sampling effort and associated cost to be evaluated. The cost effectiveness of detecting snakes has received no attention in Amazonia. Here we tested the effects of reducing sampling effort on estimates of species densities and assemblage composition. We identified snakes in seven plot systems, each standardised with 14 plots. The 250 m long centre line of each plot followed an altitudinal contour. Surveys were repeated four times in each plot and detection probabilities were estimated for the 41 species encountered. Reducing the number of observations, or the size of the sampling modules, caused significant loss of information on species densities and local patterns of variation in assemblage composition. We estimated the cost to find a snake as $ 120 U.S., but general linear models indicated the possibility of identifying differences in assemblage composition for half the overall survey costs. Decisions to reduce sampling effort depend on the importance of lost information to target-issues, and may not be the preferred option if there is the potential for identifying individual snake species requiring specific conservation actions. However, in most studies of human disturbance on species assemblages, it is likely to be more cost-effective to focus on other groups of organisms with higher detection probabilities. PMID:25147930

  10. Evaluation of Chemical Composition and Antileishmanial and Antituberculosis Activities of Essential Oils of Piper Species.

    PubMed

    Bernuci, Karine Zanoli; Iwanaga, Camila Cristina; Fernadez-Andrade, Carla Maria Mariano; Lorenzetti, Fabiana Brusco; Torres-Santos, Eduardo Caio; Faiões, Viviane Dos Santos; Gonçalves, José Eduardo; do Amaral, Wanderlei; Deschamps, Cícero; Scodro, Regiane Bertin de Lima; Cardoso, Rosilene Fressatti; Baldin, Vanessa Pietrowski; Cortez, Diógenes Aparício Garcia

    2016-12-12

    Essential oils from fresh Piperaceae leaves were obtained by hydrodistillation and analyzed by gas chromatography mass spectrometry (GC-MS), and a total of 68 components were identified. Principal components analysis results showed a chemical variability between species, with sesquiterpene compounds predominating in the majority of species analyzed. The composition of the essential oil of Piper mosenii was described for the first time. The cytotoxicity of the essential oils was evaluated in peritoneal macrophages and the oils of P. rivinoides , P. arboretum , and P. aduncum exhibited the highest values, with cytotoxic concentration at 50% (CC 50 ) > 200 µg/mL. Both P. diospyrifolium and P. aduncum displayed activity against Leishmania amazonensis , and were more selective for the parasite than for the macrophages, with a selectivity index (SI) of 2.35 and >5.52, respectively. These SI values were greater than the 1 for the standard drug pentamidine. The antileishmanial activity of the essential oils of P. diospyrifolium and P. aduncum was described for the first time. P. rivinoides, P. cernuum , and P. diospyrifolium displayed moderate activity against the Mycobacterium tuberculosis H 37 Rv bacillus, with a minimum inhibitory concentration (MIC) of 125 µg/mL. These results are relevant and suggests their potential for therapeutic purposes. Nevertheless, further studies are required to explain the exact mechanism of action of these essential oils.

  11. Effect of fabricated density and bamboo species on physical-mechanical properties of bamboo fiber bundle reinforced composites

    Treesearch

    Jiulong Xie; Jinqiu Qi; Tingxing Hu; Cornelis F. De Hoop; Chung Yun Hse; Todd F. Shupe

    2016-01-01

    Bamboo stems were subjected to a mechanical treatment process for the extraction of bamboo fiber bundles. The fiber bundles were used as reinforcement for the fabrication of high-performance composites with phenolic resins as matrix. The influence of fabricated density and bamboo species on physical–mechanical properties of bamboo fiber bundle reinforced composites (...

  12. Comparative Analysis of Leaf Trichome Structure and Composition of Epicuticular Flavonoids in Finnish Birch Species

    PubMed Central

    VALKAMA, ELENA; SALMINEN, JUHA‐PEKKA; KORICHEVA, JULIA; PIHLAJA, KALEVI

    2003-01-01

    The morphology, ultrastructure, density and distribution of trichomes on leaves of Betula pendula, B. pubescens ssp. pubescens, B. pubescens ssp. czerepanovii and B. nana were examined by means of light, scanning and transmission electron microscopy. The composition of flavonoids in ethanolic leaf surface extracts was analysed by high pressure liquid chromatography. All taxa examined contained both glandular and non‐glandular trichomes (short and/or long hairs) but differed from each other in trichome ultrastructure, density and location on the leaf. Leaves of B. pubescens were more hairy than those of B. pendula, but the latter species had a higher density of glandular trichomes. Of the two subspecies of B. pubescens, leaves of ssp. pubescens had more short hairs on the leaf surface and four times the density of glandular trichomes of leaves of ssp. czerepanovii, whereas, in the latter subspecies, short hairs occurred largely on leaf veins, as in B. nana. The glandular trichomes were peltate glands, consisting of medullar and cortical cells, which differed structurally. Cortical cells possessed numerous small, poorly developed plastids and small vacuoles, whereas medullar cells had several large plastids with well‐developed thylakoid systems and fewer vacuoles. In B. pubescens subspecies, vacuoles of the glandular cells contained osmiophilic deposits, which were probably phenolic, whereas in B. pendula, vacuoles of glandular trichomes were characterized by the presence of numerous myelin‐like membranes. The composition of epicuticular flavonoids also differed among species. The two subspecies of B. pubescens and B. nana shared the same 12 compounds, but five of these occurred only in trace amounts in B. nana. Leaf surface extracts of B. pendula contained just six flavonoids, three of which occurred only in this species. In summary, the structure, density and distribution of leaf trichomes and the composition of epicuticular flavonoids represent good

  13. The influence of tree species composition on the storage and mobility of semivolatile organic compounds in forest soils.

    PubMed

    Komprdová, Klára; Komprda, Jiří; Menšík, Ladislav; Vaňková, Lenka; Kulhavý, Jiří; Nizzetto, Luca

    2016-05-15

    Soil contamination with PCBs and PAHs in adjacent forest plots, characterized by a distinct composition in tree species (spruce only, mixed and beech only), was analyzed to investigate the influence of ecosystem type on contaminant mobility in soil under very similar climate and exposure conditions. Physical-chemical properties and contaminant concentrations in litter (L), organic (F, H) and mineral (A, B) soil horizons were analyzed. Contaminant distribution in the soil core varied both in relation to forest type and contaminant group/properties. Contaminant mobility in soil was assessed by examining the ratios of total organic carbon (TOC)-standardized concentrations across soil horizons (Enrichment factors, EFTOC) and the relationship between EFTOC and the octanol-water equilibrium partitioning coefficient (KOW). Contaminant distribution appeared to be highly unsteady, with pedogenic/biogeochemical drivers controlling contaminant mobility in organic layers and leaching controlling accumulation in mineral layers. Lighter PCBs displayed higher mobility in all forest types primarily controlled by leaching and, to a minor extent, diffusion. Pedogenic processes controlling the formation of soil horizons were found to be crucial drivers of PAHs and heavier PCBs distribution. All contaminants appeared to be more mobile in the soil of the broadleaved plot, followed by mixed canopy and spruce forest. Increasing proportion of deciduous broadleaf species in the forest can thus lead to faster degradation or the faster leaching of PAHs and PCBs. The composition of humic substances was found to be a better descriptor of contaminant concentration than TOC. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Photocatalyzed Hydrogen Evolution from Water by a Composite Catalyst of NH2 -MIL-125(Ti) and Surface Nickel(II) Species.

    PubMed

    Meyer, Kim; Bashir, Shahid; Llorca, Jordi; Idriss, Hicham; Ranocchiari, Marco; van Bokhoven, Jeroen A

    2016-09-19

    A composite of the metal-organic framework (MOF) NH 2 -MIL-125(Ti) and molecular and ionic nickel(II) species, catalyzed hydrogen evolution from water under UV light. In 95 v/v % aqueous conditions the composite produced hydrogen in quantities two orders of magnitude higher than that of the virgin framework and an order of magnitude greater than that of the molecular catalyst. In a 2 v/v % water and acetonitrile mixture, the composite demonstrated a TOF of 28 mol H 2  g(Ni) -1  h -1 and remained active for up to 50 h, sustaining catalysis for three times longer and yielding 20-fold the amount of hydrogen. Appraisal of physical mixtures of the MOF and each of the nickel species under identical photocatalytic conditions suggest that similar surface localized light sensitization and proton reduction processes operate in the composite catalyst. Both nickel species contribute to catalytic conversion, although different activation behaviors are observed. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Soil-occupancy effects of invasive and native grassland plant species on composition and diversity of mycorrhizal associations

    USGS Publications Warehouse

    Jordan, Nicholas R.; Aldrich-Wolfe, Laura; Huerd, Sheri C.; Larson, Diane L.; Muehlbauer, Gary

    2012-01-01

    Diversified grasslands that contain native plant species can produce biofuels, support sustainable grazing systems, and produce other ecosystem services. However, ecosystem service production can be disrupted by invasion of exotic perennial plants, and these plants can have soil-microbial “legacies” that may interfere with establishment and maintenance of diversified grasslands even after effective management of the invasive species. The nature of such legacies is not well understood, but may involve suppression of mutualisms between native species and soil microbes. In this study, we tested the hypotheses that legacy effects of invasive species change colonization rates, diversity, and composition of arbuscular-mycorrhizal fungi (AMF) associated with seedlings of co-occurring invasive and native grassland species. In a glasshouse, experimental soils were conditioned by cultivating three invasive grassland perennials, three native grassland perennials, and a native perennial mixture. Each was grown separately through three cycles of growth, after which we used T-RFLP analysis to characterize AMF associations of seedlings of six native perennial and six invasive perennial species grown in these soils. Legacy effects of soil conditioning by invasive species did not affect AMF richness in seedling roots, but did affect AMF colonization rates and the taxonomic composition of mycorrhizal associations in seedling roots. Moreover, native species were more heavily colonized by AMF and roots of native species had greater AMF richness (number of AMF operational taxonomic units per seedling) than did invasive species. The invasive species used to condition soil in this experiment have been shown to have legacy effects on biomass of native seedlings, reducing their growth in this and a previous similar experiment. Therefore, our results suggest that successful plant invaders can have legacies that affect soil-microbial associations of native plants and that these effects

  16. Process for derivatizing carbon nanotubes with diazonium species and compositions thereof

    NASA Technical Reports Server (NTRS)

    Bahr, Jeffrey L. (Inventor); Tour, James M. (Inventor); Yang, Jiping (Inventor)

    2011-01-01

    Methods for the chemical modification of carbon nanotubes involve the derivatization of multi- and single-wall carbon nanotubes, including small diameter (ca. 0.7 nm) single-wall carbon nanotubes, with diazonium species. The method allows the chemical attachment of a variety of organic compounds to the side and ends of carbon nanotubes. These chemically modified nanotubes have applications in polymer composite materials, molecular electronic applications, and sensor devices. The methods of derivatization include electrochemical induced reactions, thermally induced reactions, and photochemically induced reactions. Moreover, when modified with suitable chemical groups, the derivatized nanotubes are chemically compatible with a polymer matrix, allowing transfer of the properties of the nanotubes (such as, mechanical strength or electrical conductivity) to the properties of the composite material as a whole. Furthermore, when modified with suitable chemical groups, the groups can be polymerized to form a polymer that includes carbon nanotubes.

  17. A power analysis for multivariate tests of temporal trend in species composition.

    PubMed

    Irvine, Kathryn M; Dinger, Eric C; Sarr, Daniel

    2011-10-01

    Long-term monitoring programs emphasize power analysis as a tool to determine the sampling effort necessary to effectively document ecologically significant changes in ecosystems. Programs that monitor entire multispecies assemblages require a method for determining the power of multivariate statistical models to detect trend. We provide a method to simulate presence-absence species assemblage data that are consistent with increasing or decreasing directional change in species composition within multiple sites. This step is the foundation for using Monte Carlo methods to approximate the power of any multivariate method for detecting temporal trends. We focus on comparing the power of the Mantel test, permutational multivariate analysis of variance, and constrained analysis of principal coordinates. We find that the power of the various methods we investigate is sensitive to the number of species in the community, univariate species patterns, and the number of sites sampled over time. For increasing directional change scenarios, constrained analysis of principal coordinates was as or more powerful than permutational multivariate analysis of variance, the Mantel test was the least powerful. However, in our investigation of decreasing directional change, the Mantel test was typically as or more powerful than the other models.

  18. Total elemental composition of soils contaminated with wastewater irrigation by combining IBA techniques

    NASA Astrophysics Data System (ADS)

    Huerta, L.; Contreras-Valadez, R.; Palacios-Mayorga, S.; Miranda, J.; Calva-Vasquez, G.

    2002-04-01

    The purpose of this work was to obtain the total elemental composition of agricultural soils irrigated with well water and wastewater. The studied area is located in the Valle del Mezquital in Hidalgo State, Mexico. The studied soils were collected, every two months during one year. Particle induced X-ray emission (PIXE), Rutherford backscattering spectrometry (RBS) and nuclear reaction analysis (NRA) were applied for elemental analysis. PIXE analyses gave elemental contents of major and trace elements (Al, Si, P, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Br, Rb, Sr, Y, Zr, and Pb). Total concentrations of Na, Mg, C, N and O were obtained by RBS and NRA. PIXE analyses were carried out with 2 MeV proton beams, RBS with 2 MeV helium ions, while NRA was applied with a 1.2 MeV deuterium beam. Results indicated that heavy metal total concentrations exceed the critical soil total concentrations according to environmental regulations.

  19. Prey dispersal rate affects prey species composition and trait diversity in response to multiple predators in metacommunities.

    PubMed

    Howeth, Jennifer G; Leibold, Mathew A

    2010-09-01

    1. Recent studies indicate that large-scale spatial processes can alter local community structuring mechanisms to determine local and regional assemblages of predators and their prey. In metacommunities, this may occur when the functional diversity represented in the regional predator species pool interacts with the rate of prey dispersal among local communities to affect prey species diversity and trait composition at multiple scales. 2. Here, we test for effects of prey dispersal rate and spatially and temporally heterogeneous predation from functionally dissimilar predators on prey structure in pond mesocosm metacommunities. An experimental metacommunity consisted of three pond mesocosm communities supporting two differentially size-selective invertebrate predators and their zooplankton prey. In each metacommunity, two communities maintained constant predation and supported either Gyrinus sp. (Coleoptera) or Notonecta ungulata (Hemiptera) predators generating a spatial prey refuge while the third community supported alternating predation from Gyrinus sp. and N. ungulata generating a temporal prey refuge. Mesocosm metacommunities were connected at either low (0.7% day(-1)) or high (10% day(-1)) planktonic prey dispersal. The diversity, composition and body size of zooplankton prey were measured at local and regional (metacommunity) scales. 3. Metacommunities experiencing the low prey dispersal rate supported the greatest regional prey species diversity (H') and evenness (J'). Neither dispersal rate nor predation regime affected local prey diversity or evenness. The spatial prey refuge at low dispersal maintained the largest difference in species composition and body size diversity between communities under Gyrinus and Notonecta predation, suggesting that species sorting was operating at the low dispersal rate. There was no effect of dispersal rate on species diversity or body size distribution in the temporal prey refuge. 4. The frequency distribution, but not

  20. Legacy effects of no-analogue disturbances alter plant community diversity and composition in semi-arid sagebrush steppe

    USGS Publications Warehouse

    Ripplinger, Julie; Franklin, Janet; Edwards, Thomas C.

    2015-01-01

    Questions(i) What role does the type of managed disturbance play in structuring sagebrush steppe plant communities? (ii) How does the composition of post-disturbance plant communities change with time since disturbance? (iii) Does plant community diversity change over time following managed disturbance?LocationField study within the sagebrush steppe ecosystem. Rich County, Utah, USA.MethodsWe developed a chronosequence spanning up to 50 yrs post-treatment to study sagebrush steppe vegetation dynamics. Direct ordination was used to examine plant community composition by managed disturbance type and time since disturbance, and factorial analysis of covariance was used to examine diversity dynamics following disturbance. Indicator species values were calculated in order to identify characteristic species for each disturbance type.ResultsPlant communities experienced a shift toward distinct community composition for each of the three managed disturbance types, and gave no indication of returning to untreated community composition or diversity. Small post-disturbance increases in the number of non-native grass species were observed in the treatments relative to reference, with native forb species making the largest contribution to altered composition. On fire- and chemically-treated sites the proportional native forb species richness increased over time since disturbance, while the proportional contribution of non-native forbs to total species richness decreased. For all three treatment types, native grasses contributed less on average to total richness than on reference sites, while non-native grasses made up a higher proportion of total richness.ConclusionsCommon shrubland management techniques have legacy effects on the composition and diversity of sagebrush steppe plant communities, and no-analogue disturbances, such as chemical or mechanical treatments, have more pronounced legacy effects than treatments similar to natural disturbance regimes (fire). This study

  1. Multi-sensor data fusion for estimating forest species composition and abundance in northern Minnesota

    Treesearch

    Peter P. Wolter; Phillip A. Townsend

    2011-01-01

    The magnitude, duration, and frequency of forest disturbance caused by the spruce budworm and forest tent caterpillar in northern Minnesota and neighboring Ontario, Canada have increased over the last century due to a shift in forest species composition linked to historical fire suppression, forest management, and pesticide application that has fostered increased...

  2. Subalpine Forest Carbon Cycling Short- and Long-Term Influence ofClimate and Species

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kueppers, L.; Harte, J.

    2005-08-23

    Ecosystem carbon cycle feedbacks to climate change comprise one of the largest remaining sources of uncertainty in global model predictions of future climate. Both direct climate effects on carbon cycling and indirect effects via climate-induced shifts in species composition may alter ecosystem carbon balance over the long term. In the short term, climate effects on carbon cycling may be mediated by ecosystem species composition. We used an elevational climate and tree species composition gradient in Rocky Mountain subalpine forest to quantify the sensitivity of all major ecosystem carbon stocks and fluxes to these factors. The climate sensitivities of carbon fluxesmore » were species-specific in the cases of relative above ground productivity and litter decomposition, whereas the climate sensitivity of dead wood decay did not differ between species, and total annual soil CO2 flux showed no strong climate trend. Lodge pole pine relative productivity increased with warmer temperatures and earlier snowmelt, while Engelmann spruce relative productivity was insensitive to climate variables. Engelmann spruce needle decomposition decreased linearly with increasing temperature(decreasing litter moisture), while lodgepole pine and subalpine fir needle decay showed a hump-shaped temperature response. We also found that total ecosystem carbon declined by 50 percent with a 2.88C increase in mean annual temperature and a concurrent 63 percent decrease ingrowing season soil moisture, primarily due to large declines in mineral soil and dead wood carbon. We detected no independent effect of species composition on ecosystem C stocks. Overall, our carbon flux results suggest that, in the short term, any change in subalpine forest net carbon balance will depend on the specific climate scenario and spatial distribution of tree species. Over the long term, our carbon stock results suggest that with regional warming and drying, Rocky Mountain subalpine forest will be a net source of

  3. Quantification of total and particulate dimethylsulfoniopropionate (DMSP) in five Bermudian coral species across a depth gradient

    NASA Astrophysics Data System (ADS)

    Yost, D. M.; Jones, R.; Rowe, C. L.; Mitchelmore, Carys Louise

    2012-06-01

    The symbiotic dinoflagellate microalgae of corals ( Symbiodinium spp.) contain high concentrations of dimethylsulfoniopropionate (DMSP), a multifunctional metabolite commonly found in many species of marine algae and dinoflagellates. A photoprotective antioxidant function for DMSP and its breakdown products has often been inferred in algae, but its role(s) in the coral-algal symbiosis remains elusive. To examine potential correlations between environmental and physiological parameters and DMSP, total DMSP (DMSPt, from the host coral and zooxanthellae), particulate DMSP (DMSPp, from the zooxanthellae only), coral surface area, and total protein, as well as zooxanthellae density, chlorophyll concentration, cell volume and genotype (i.e., clade) were measured in five coral species from the Diploria- Montastraea- Porites species complex in Bermuda along a depth gradient of 4, 12, 18, and 24 m. DMSPt concentrations were consistently greater than DMSPp concentrations in all species suggesting the possible translocation of DMSP from symbiont to host. D. labyrinthiformis was notably different from the other corals examined, showing DMSPp and DMSPt increases (per coral surface area or tissue biomass) with increasing water depth. However, overall, there were no consistent depth-related patterns in DMSPp and DMSPt concentrations. Further research, investigating dimethylsulfide (DMS), dimethylsulfoxide, and acrylate levels and DMSP-lyase activity in correlation with other biomarker endpoints that have been shown to be depth (i.e., temperature and light) responsive are needed to substantiate the significance of these findings.

  4. Evaluation of Total Dissolved Solids and Specific Conductance Water Quality Targets with Paired Single-Species and Mesocosm Community Exposures

    EPA Science Inventory

    Isolated single-species exposures were conducted in parallel with 42 d mesocosm dosing studies that measured in-situ and whole community responses to different recipes of excess total dissolved solids (TDS). The studies were conducted with cultured species and native taxa from mo...

  5. Comparing Single species Toxicity Tests to Mesocosm Community-Level Responses to Total Dissolved Solids Comprised of Different Major Ions

    EPA Science Inventory

    Total Dissolved Solids (TDS) dosing studies representing different sources of ions were conducted from 2011-2015. Emergence responses in stream mesocosms were compared to single-species exposures using a whole effluent testing (WET) format and an ex-situ method (single species te...

  6. Fatty acid composition of Juniperus species (Juniperus section) native to Turkey.

    PubMed

    Güvenç, Aysegül; Küçükboyaci, Nurgün; Gören, Ahmet Ceyhan

    2012-07-01

    Fatty acid compositions of seeds of five taxa of the Juniperus section of the genus Juniperus L. (Cupressaceae), i. e. J. drupacea Lab., J. communis L. var. communis, J. communis var. saxatilis Pall., J. oxycedrus L. subsp. oxycedrus, and J. oxycedrus subsp. macrocarpa (Sibth. & Sm.) Ball, were investigated. Methyl ester derivatized fatty acids of the lipophylic extracts of the five species were comparatively analyzed by capillary gas chromatography-mass spectrometry (GC-MS). Juniperus taxa showed uniform fatty acid patterns, among which linoleic (25.8 - 32.5%), pinolenic (11.9 - 24.1%) and oleic acids (12.4 - 17.2%) were determined to be the main fractions in the seed oils. Juniperonic acid was found to be remarkably high in J. communis var. saxatilis (11.4%), J. oxycedrus subsp. oxycedrus (10.4%), and J. communis var. communis (10.1%). To the best of our knowledge, the present work discloses the first report on the fatty acid compositions of seeds of this Juniperus section grown in Turkey.

  7. pCO2 effects on species composition and growth of an estuarine phytoplankton community

    NASA Astrophysics Data System (ADS)

    Grear, Jason S.; Rynearson, Tatiana A.; Montalbano, Amanda L.; Govenar, Breea; Menden-Deuer, Susanne

    2017-05-01

    The effects of ongoing changes in ocean carbonate chemistry on plankton ecology have important implications for food webs and biogeochemical cycling. However, conflicting results have emerged regarding species-specific responses to pCO2 enrichment and thus community responses have been difficult to predict. To assess community level effects (e.g., production) of altered carbonate chemistry, studies are needed that capitalize on the benefits of controlled experiments but also retain features of intact ecosystems that may exacerbate or ameliorate the effects observed in single-species or single cohort experiments. We performed incubations of natural plankton communities from Narragansett Bay, RI, USA in winter at ambient bay temperatures (5-13 °C), light and nutrient concentrations. Three levels of controlled and constant CO2 concentrations were imposed, simulating past, present and future conditions at mean pCO2 levels of 224, 361, and 724 μatm respectively. Samples for carbonate analysis, chlorophyll a, plankton size-abundance, and plankton species composition were collected daily and phytoplankton growth rates in three different size fractions (<5, 5-20, and >20 μm) were measured at the end of the 7-day incubation period. Community composition changed during the incubation period with major increases in relative diatom abundance, which were similar across pCO2 treatments. At the end of the experiment, 24-hr growth responses to pCO2 levels varied as a function of cell size. The smallest size fraction (<5 μm) grew faster at the elevated pCO2 level. In contrast, the 5-20 μm size fraction grew fastest in the Present treatment and there were no significant differences in growth rate among treatments in the >20 μm size fraction. Cell size distribution shifted toward smaller cells in both the Past and Future treatments but remained unchanged in the Present treatment. Similarity in Past and Future treatments for cell size distribution and growth rate (5-20 μm size

  8. Total selenium and selenium species in irrigation drain inflows to the Salton Sea, California, April and July 2008

    USGS Publications Warehouse

    May, Thomas W.; Walther, Michael J.; Saiki, Michael K.; Brumbaugh, William G.

    2009-01-01

    This report presents the results for two sampling periods (April 2008 and July 2008) during a 4-year monitoring program to characterize selenium concentrations in selected irrigation drains flowing into the Salton Sea, California. Total selenium, selenium species (dissolved selenite, selenate, organoselenium), and total suspended solids were determined in water samples and total selenium was determined in water column particulates and in sediment, detritus, and biota that included algae, plankton, midge larvae (family, Chironomidae), and two fish species - western mosquitofish (Gambusia affinis) and sailfin molly (Poecilia latipinna). In addition, sediments were analyzed for percent total organic carbon and particle size. Mean total selenium concentrations in water for both sampling periods ranged from 1.93 to 44.2 micrograms per liter, predominately as selenate, which is typical of waters where selenium is leached out of selenium-containing marine shales and associated soils under alkaline and oxidizing conditions. Total selenium concentrations (micrograms per gram dry weight) ranged as follows: algae, 0.75 to 3.39; plankton, 0.88 to 4.03; midges, 2.52 to 44.3; fish, 3.37 to 18.9; detritus, 1.11 to 13.6; sediment, 0.11 to 8.93.

  9. Species composition influences management outcomes following mountain pine beetle in lodgepole pine-dominated forests

    Treesearch

    Kristen Pelz; C. C. Rhoades; R. M. Hubbard; M. A. Battaglia; F. W. Smith

    2015-01-01

    Mountain pine beetle outbreaks have killed lodgepole pine on more than one million hectares of Colorado and southern Wyoming forest during the last decade and have prompted harvest operations throughout the region. In northern Colorado, lodgepole pine commonly occurs in mixed stands with subalpine fir, Engelmann spruce, and aspen. Variation in tree species composition...

  10. Nutrient addition shifts plant community composition towards earlier flowering species in some prairie ecoregions in the U.S. Central Plains

    PubMed Central

    Mortensen, Brent; Fay, Philip; Hagenah, Nicole; Knops, Johannes; La Pierre, Kimberly; Laungani, Ramesh; Lind, Eric; McCulley, Rebecca; Power, Sally; Seabloom, Eric; Tognetti, Pedro

    2017-01-01

    The distribution of flowering across the growing season is governed by each species’ evolutionary history and climatic variability. However, global change factors, such as eutrophication and invasion, can alter plant community composition and thus change the distribution of flowering across the growing season. We examined three ecoregions (tall-, mixed, and short-grass prairie) across the U.S. Central Plains to determine how nutrient (nitrogen (N), phosphorus, and potassium (+micronutrient)) addition alters the temporal patterns of plant flowering traits. We calculated total community flowering potential (FP) by distributing peak-season plant cover values across the growing season, allocating each species’ cover to only those months in which it typically flowers. We also generated separate FP profiles for exotic and native species and functional group. We compared the ability of the added nutrients to shift the distribution of these FP profiles (total and sub-groups) across the growing season. In all ecoregions, N increased the relative cover of both exotic species and C3 graminoids that flower in May through August. The cover of C4 graminoids decreased with added N, but the response varied by ecoregion and month. However, these functional changes only aggregated to shift the entire community’s FP profile in the tall-grass prairie, where the relative cover of plants expected to flower in May and June increased and those that flower in September and October decreased with added N. The relatively low native cover in May and June may leave this ecoregion vulnerable to disturbance-induced invasion by exotic species that occupy this temporal niche. There was no change in the FP profile of the mixed and short-grass prairies with N addition as increased abundance of exotic species and C3 graminoids replaced other species that flower at the same time. In these communities a disturbance other than nutrient addition may be required to disrupt phenological patterns

  11. Effect of litter, leaf cover and cover of basal internodes of the dominant species Molinia caerulea on seedling recruitment and established vegetation

    NASA Astrophysics Data System (ADS)

    Janeček, Štěpán; Lepš, Jan

    2005-09-01

    The effects of litter removal, leaf cover of established plants and cover of basal internodes of a dominant species Molinia caerulea on seedling germination and the dynamics of established plants were studied in a field experiment in an oligotrophic wet meadow. Although the negative influence of litter on total seedling number and seedling species composition was non-significant, litter significantly affected the dynamics of the established vegetation and caused inhibition of total leaf cover development. The effects of total leaf cover of established plants on seedling establishment changed during the vegetation season. Whereas the effect of total leaf cover was positive at the start and in the middle of the vegetation season, at the end the total leaf cover negatively affected seedling establishment. Both total leaf cover and cover of basal internodes affected seedling composition. Effects of these two variables were statistically separable suggesting that they are based on different mechanisms. The response of seedling establishment to these factors was species specific and, consequently, our data support the hypothesis that that biotically generated spatial heterogeneity can promote species co-existence through the differentiation of species regeneration niches.

  12. Viral Impacts on Total Abundance and Clonal Composition of the Harmful Bloom-Forming Phytoplankton Heterosigma akashiwo

    PubMed Central

    Tarutani, Kenji; Nagasaki, Keizo; Yamaguchi, Mineo

    2000-01-01

    Recent observations that viruses are very abundant and biologically active components in marine ecosystems suggest that they probably influence various biogeochemical and ecological processes. In this study, the population dynamics of the harmful bloom-forming phytoplankton Heterosigma akashiwo (Raphidophyceae) and the infectious H. akashiwo viruses (HaV) were monitored in Hiroshima Bay, Japan, from May to July 1998. Concurrently, a number of H. akashiwo and HaV clones were isolated, and their virus susceptibilities and host ranges were determined through laboratory cross-reactivity tests. A sudden decrease in cell density of H. akashiwo was accompanied by a drastic increase in the abundance of HaV, suggesting that viruses contributed greatly to the disintegration of the H. akashiwo bloom as mortality agents. Despite the large quantity of infectious HaV, however, a significant proportion of H. akashiwo cells survived after the bloom disintegration. The viral susceptibility of H. akashiwo isolates demonstrated that the majority of these surviving cells were resistant to most of the HaV clones, whereas resistant cells were a minor component during the bloom period. Moreover, these resistant cells were displaced by susceptible cells, presumably due to viral infection. These results demonstrated that the properties of dominant cells within the H. akashiwo population change during the period when a bloom is terminated by viral infection, suggesting that viruses also play an important role in determining the clonal composition and maintaining the clonal diversity of H. akashiwo populations. Therefore, our data indicate that viral infection influences the total abundance and the clonal composition of one host algal species, suggesting that viruses are an important component in quantitatively and qualitatively controlling phytoplankton populations in natural marine environments. PMID:11055943

  13. Nonlinear and Threshold Responses of Grassland Productivity and Species Composition to Increased CO2 Vary with Soil Type

    NASA Astrophysics Data System (ADS)

    Fay, P. A.; Jin, V.; Jackson, R. B.; Gill, R. A.; Way, D.; Polley, W.

    2011-12-01

    Climate change is likely to cause nonlinear responses in ecosystem function and threshold changes in species composition. Here we report aboveground net primary productivity (ANPP) responses to a continuous CO2 concentration gradient (250 to 500 μL L-1,) in experimental grassland communities on three soils differing in water holding capacity and other properties. Communities consisting of four C4 grasses, two C3 forbs, and one legume were established on a lowland clay (vertisol, n=32), an upland clay (mollisol, n=32), and an alluvial sand (alfisol, n=16). The communities were positioned in a stratified random design in the CO2 gradient for five growing seasons, and were irrigated to mimic the average growing season rainfall regime for the study site in Central Texas. ANPP increased with CO2 almost two-fold more on the upland clay and alluvial sand than on the lowland clay (p < 0.0001), because of strong linear responses to CO2 on these soils (R2 = 0.50 to 0.59, p < 0.002) compared to a saturating response to CO2 on the lowland clay (R2 = 0.48, p= 0.01). On the two more responsive soils, the mesic tallgrass Sorghastrum nutans replaced the more drought adapted mid-grass Bouteloua curtipendula at elevated CO2, while B. curtipendula largely replaced S. nutans at low CO2, especially on the upland clay. Evidence for a similar composition change was not found on the lowland clay. Thus, two soils displayed a threshold change in community composition that accounted for up to 57% of variation in ANPP for those soils. Variation in ANPP and species composition with CO2 were accompanied by linear increases in soil water content (SWC, 0 - 20 cm, volumetric), most strongly on the alluvial sand (R2 = 0.39, p < 0.009) and by weak decreases with CO2 in soil N. Structural equation models explained 34 to 52% of the variation in ANPP, and indicated that CO2 effects on ANPP on the upland clay were primarily explained by CO2 effects on species composition, and on the alluvial sand by CO

  14. Temperate grassland songbird species accumulate incrementally along a gradient of primary productivity

    PubMed Central

    Srivastava, Diane S.; McCallum, Cindy; Fraser, Lauchlan H.; Turkington, Roy

    2017-01-01

    Global analyses of bird communities along elevation gradients suggest that bird diversity on arid mountains is primarily limited by water availability, not temperature or altitude. However, the mechanism by which water availability, and subsequently primary productivity, increases bird diversity is still unclear. Here we evaluate two possible mechanisms from species-energy theory. The more individuals hypothesis proposes that a higher availability of resources increases the total number of individuals that can be supported, and therefore the greater number of species that will be sampled. By contrast, the more specialization hypothesis proposes that increasing resource availability will permit specialists to exploit otherwise rare resources, thus increasing total diversity. We used 5 years of surveys of grassland songbird communities along an elevational gradient in British Columbia, Canada, to distinguish between these hypotheses. Vegetation changed markedly in composition along the gradient and contrary to the expectations of the more specialization hypothesis, bird community composition was remarkably constant. However, both total abundance and species richness of birds increased with increasing water availability to plants. When we used rarefaction to correct species richness for differences in total abundance, much of the increase in bird diversity was lost, consistent with the expectations of the more individuals hypothesis. Furthermore, high species richness was associated with reductions in territory size of common bird species, rather than the fine-scale spatial partitioning of the landscape. This suggests that bird diversity increases when greater resource availability allows higher densities rather than greater habitat specialization. These results help explain a pervasive global pattern in bird diversity on arid mountains, and suggest that in such landscapes conservation of grassland birds is strongly linked to climate and hydrology. PMID:29059252

  15. Triacylglyceride composition and fatty acyl saturation profile of a psychrophilic and psychrotolerant fungal species grown at different temperatures.

    PubMed

    Pannkuk, Evan L; Blair, Hannah B; Fischer, Amy E; Gerdes, Cheyenne L; Gilmore, David F; Savary, Brett J; Risch, Thomas S

    2014-01-01

    Pseudogymnoascus destructans is a psychrophilic fungus that infects cutaneous tissues in cave dwelling bats, and it is the causal agent for white nose syndrome (WNS) in North American (NA) bat populations. Geomyces pannorum is a related psychrotolerant keratinolytic species that is rarely a pathogen of mammals. In this study, we grew P. destructans and G. pannorum in static liquid cultures at favourable and suboptimal temperatures to: 1) determine if triacylglyceride profiles are species-specific, and 2) determine if there are differences in fatty acyl (FA) saturation levels with respect to temperature. Total lipids isolated from both fungal spp. were separated by thin-layer chromatography and determined to be primarily sterols (∼15 %), free fatty acids (FFAs) (∼45 %), and triacylglycerides (TAGs) (∼50 %), with minor amounts of mono-/diacylglycerides and sterol esters. TAG compositions were profiled by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF). Total fatty acid methyl esters (FAMEs) and acyl lipid unsaturation levels were determined by gas chromatography-mass spectrometry (GC-MS). Pseudogymnoascus destructans produced higher proportions of unsaturated 18C fatty acids and TAGs than G. pannorum. Pseudogymnoascus destructans and G. pannorum produced up to a two-fold increase in 18:3 fatty acids at 5 °C than at higher temperatures. TAG proportion for P. destructans at upper and lower temperature growth limits was greater than 50 % of total dried mycelia mass. These results indicate fungal spp. alter acyl lipid unsaturation as a strategy to adapt to cold temperatures. Differences between their glycerolipid profiles also provide evidence for a different metabolic strategy to support psychrophilic growth, which may influence P. destructans' pathogenicity to bats. Copyright © 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  16. [The research of biodegradation of a composite material used in reconstructive and reparative surgery of maxillofacial area].

    PubMed

    Malanchuk, V O; Astapenko, O O; Halatenko, N A; Rozhnova, R A

    2013-09-01

    Dates about the research of biodegradation of epoxy-polyurethane composite material used in reconstructive and reparative surgery of maxillofacial area are reflected in the article. Was founded: 1) notable biodegradation of species from epoxy-polyurethane composition in the term of observation up to 6 months was not founded. That testifies their preservation of physical and mechanical properties. 2) founded, that in species from epoxy-polyurethane composition, which contain levamisole, processes of biodegradation are faster then in species from pure epoxy-polyurethane composition and in species from epoxy-polyurethane composition with hydroxyapatite; 3) material from epoxy-polyurethane composition, which contains levamisole and hydroxyapatite, stays in biological environment in small quantity of petty fragments during the incubation in term of 2 years. So, it biodegrades practically totally. Authors suggest on the basis of achieved information, that the use of epoxy-polyurethane constructions that biodegrade, is pertinently in reconstructive maxillofacial surgery.

  17. The influence of habitat structure on bird species composition in lowland malaysian rain forests.

    PubMed

    Mansor, Mohammad Saiful; Sah, Shahrul Anuar Mohd

    2012-05-01

    Bird surveys were conducted in the Bukit Kepala Gajah limestone area in Lenggong, Perak from July 2010 to January 2011. The study area was divided into three zones: forest edge, forest intermediate and forest interior. A point-count distance sampling method was used in the bird surveys. The study recorded 7789 detections, representing 100 bird species belonging to 28 families. Pycnonotidae, Timaliidae and Nectariniidae were the dominant families overall and showed the highest number of observations recorded in the study area whereas Motacillidae showed the fewest observations. The bird species were grouped into three feeding guilds: insectivores, frugivores and others (omnivores, carnivores, nectarivores and granivores). The species richness of insectivorous birds differed significantly among the forest zones sampled (Kruskal-Wallis: α=0.05, H=10.979, d.f.=2, p=0.004), with more insectivorous birds occurring in the forest interior. No significant differences were found among the zones in the species richness of either the frugivore guild or the composite others guild.

  18. The Influence of Habitat Structure on Bird Species Composition in Lowland Malaysian Rain Forests

    PubMed Central

    Mansor, Mohammad Saiful; Sah, Shahrul Anuar Mohd

    2012-01-01

    Bird surveys were conducted in the Bukit Kepala Gajah limestone area in Lenggong, Perak from July 2010 to January 2011. The study area was divided into three zones: forest edge, forest intermediate and forest interior. A point-count distance sampling method was used in the bird surveys. The study recorded 7789 detections, representing 100 bird species belonging to 28 families. Pycnonotidae, Timaliidae and Nectariniidae were the dominant families overall and showed the highest number of observations recorded in the study area whereas Motacillidae showed the fewest observations. The bird species were grouped into three feeding guilds: insectivores, frugivores and others (omnivores, carnivores, nectarivores and granivores). The species richness of insectivorous birds differed significantly among the forest zones sampled (Kruskal-Wallis: α=0.05, H=10.979, d.f.=2, p=0.004), with more insectivorous birds occurring in the forest interior. No significant differences were found among the zones in the species richness of either the frugivore guild or the composite others guild. PMID:24575221

  19. Prospects for quantifying structure, floristic composition and species richness of tropical forests

    USGS Publications Warehouse

    Gillespie, T.W.; Brock, J.; Wright, C.W.

    2004-01-01

    Airborne spectral and light detection and ranging (lidar) sensors have been used to quantify biophysical characteristics of tropical forests. Lidar sensors have provided high-resolution data on forest height, canopy topography, volume, and gap size; and provided estimates on number of strata in a forest, successional status of forests, and above-ground biomass. Spectral sensors have provided data on vegetation types, foliar biochemistry content of forest canopies, tree and canopy phenology, and spectral signatures for selected tree species. A number of advances are theoretically possible with individual and combined spectral and lidar sensors for the study of forest structure, floristic composition and species richness. Delineating individual canopies of over-storey trees with small footprint lidar and discrimination of tree architectural types with waveform distributions is possible and would provide scientists with a new method to study tropical forest structure. Combined spectral and lidar data can be used to identify selected tree species and identify the successional status of tropical forest fragments in order to rank forest patches by levels of species richness. It should be possible in the near future to quantify selected patterns of tropical forests at a higher resolution than can currently be undertaken in the field or from space. ?? 2004 Taylor and Francis Ltd.

  20. Interactions of elevation, aspect, and slope in models of forest species composition and productivity

    Treesearch

    Albert R. Stage; Christian Salas

    2007-01-01

    We present a linear model for the interacting effects of elevation, aspect, and slope for use in predicting forest productivity or species composition. The model formulation we propose integrates interactions of these three factors in a mathematical expression representing their combined effect in terms of a cosine function of aspect with a phase shift and amplitude...

  1. In vitro biological activity and essential oil composition of four indigenous South African Helichrysum species.

    PubMed

    Lourens, A C U; Reddy, D; Başer, K H C; Viljoen, A M; Van Vuuren, S F

    2004-12-01

    Helichrysum species are used widely to treat various medical conditions. In this study, the anti-microbial, anti-oxidant (DPPH assay) and anti-inflammatory activity (5-lipoxygenase assay) of Helichrysum dasyanthum, Helichrysum felinum, Helichrysum excisum and Helichrysum petiolare were investigated. The essential oil compositions of these species were determined. The acetone and methanol extracts as well as the essential oils exhibited activity against Gram-positive bacteria, while both the methanol and acetone extracts of all four species were active in the anti-oxidant assay. The essential oils, on the other hand, displayed activity in the 5-lipoxygenase assay, which was used as an indication of anti-inflammatory activity. Two extracts exhibited promising activity in the anti-microbial assay, the acetone extract of Helichrysum dasyanthum with a MIC value of 15.63 microg/ml and the methanol extract of Helichrysum excisum with a MIC value of 62.5 microg/ml. The acetone extract of Helichrysum dasyanthum was the most active free radical scavenger in the DPPH assay (IC(50) of 9.53 microg/ml) while values for the anti-inflammatory activity of the essential oils ranged between 25 and 32 microg/ml. The essential oil compositions of three species (Helichrysum dasyanthum, Helichrysum excisum and Helichrysum petiolare) were dominated by the presence of monoterpenes such as alpha-pinene, 1,8-cineole and p-cymene. In the oil of Helichrysum felinum, monoterpenes were largely absent. Its profile consisted of a variety of sesquiterpenes in low concentrations with beta-caryophyllene dominating.

  2. HS/GC-MS analyzed chemical composition of the aroma of fruiting bodies of two species of genus Lentinus (Higher Basidiomycetes).

    PubMed

    Mata, Gerardo; Valdez, Karina; Mendoza, Remedios; Trigos, Ángel

    2014-01-01

    The chemical composition of the aroma of fresh fruiting bodies of the cultivated mushroom Lentinus boryanus is described here and compared with medicinal shiitake mushroom L. edodes. Volatile compounds were analyzed through headspace sampling coupled with gas chromatography-mass spectrometry. The mushrooms under study were grown on different substrates based on barley straw, sugarcane bagasse, oak wood sawdust, and beech leaf litter. It was determined that L. boryanus as well as L. edodes contain an abundant amount of a volatile compound identified as 3-octanone with a sweet fruity aroma. On the other hand, only L. boryanus produced 3-octanol a characteristic aroma of cod liver oil. In total, 10 aromatic compounds were identified, some of which were obtained exclusively in one species or substrate.

  3. Mind the gut: genomic insights to population divergence and gut microbial composition of two marine keystone species.

    PubMed

    Fietz, Katharina; Rye Hintze, Christian Olaf; Skovrind, Mikkel; Kjærgaard Nielsen, Tue; Limborg, Morten T; Krag, Marcus A; Palsbøll, Per J; Hestbjerg Hansen, Lars; Rask Møller, Peter; Gilbert, M Thomas P

    2018-05-02

    Deciphering the mechanisms governing population genetic divergence and local adaptation across heterogeneous environments is a central theme in marine ecology and conservation. While population divergence and ecological adaptive potential are classically viewed at the genetic level, it has recently been argued that their microbiomes may also contribute to population genetic divergence. We explored whether this might be plausible along the well-described environmental gradient of the Baltic Sea in two species of sand lance (Ammodytes tobianus and Hyperoplus lanceolatus). Specifically, we assessed both their population genetic and gut microbial composition variation and investigated not only which environmental parameters correlate with the observed variation, but whether host genome also correlates with microbiome variation. We found a clear genetic structure separating the high-salinity North Sea from the low-salinity Baltic Sea sand lances. The observed genetic divergence was not simply a function of isolation by distance, but correlated with environmental parameters, such as salinity, sea surface temperature, and, in the case of A. tobianus, possibly water microbiota. Furthermore, we detected two distinct genetic groups in Baltic A. tobianus that might represent sympatric spawning types. Investigation of possible drivers of gut microbiome composition variation revealed that host species identity was significantly correlated with the microbial community composition of the gut. A potential influence of host genetic factors on gut microbiome composition was further confirmed by the results of a constrained analysis of principal coordinates. The host genetic component was among the parameters that best explain observed variation in gut microbiome composition. Our findings have relevance for the population structure of two commercial species but also provide insights into potentially relevant genomic and microbial factors with regards to sand lance adaptation across

  4. Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey.

    PubMed

    Agar, Osman Tuncay; Dikmen, Miris; Ozturk, Nilgun; Yilmaz, Mustafa Abdullah; Temel, Hamdi; Turkmenoglu, Fatma Pinar

    2015-09-30

    Turkey is one of the most important centers of diversity for the genus Achillea L. in the world. Keeping in mind the immense medicinal importance of phenols, in this study, three species growing in Turkey, A. coarctata Poir. (AC), A. kotschyi Boiss. subsp. kotschyi (AK) and A. lycaonica Boiss. & Heldr. (AL) were evaluated for their phenolic compositions, total phenolic contents (TPC), antioxidant properties, wound healing potencies on NIH-3T3 fibroblasts and cytotoxic effects on MCF-7 human breast cancer cells. Comprehensive LC-MS/MS analysis revealed that AK was distinctively rich in chlorogenic acid, hyperoside, apigenin, hesperidin, rutin, kaempferol and luteolin (2890.6, 987.3, 797.0, 422.5, 188.1, 159.4 and 121.2 µg analyte/g extract, respectively). The findings exhibited a strong correlation between TPC and both free radical scavenging activity and total antioxidant capacity (TAC). Among studied species, the highest TPC (148.00 mg GAE/g extract) and TAC (2.080 UAE), the strongest radical scavenging (EC50 = 32.63 μg/mL), the most prominent wound healing and most abundant cytotoxic activities were observed with AK. The results suggested that AK is a valuable source of flavonoids and chlorogenic acid with important antioxidant, wound healing and cytotoxic activities. These findings warrant further studies to assess the potential of AK as a bioactive source that could be exploited in pharmaceutical, cosmetics and food industries.

  5. Effects of habitat composition and landscape structure on worker foraging distances of five bumble bee species.

    PubMed

    Redhead, John W; Dreier, Stephanie; Bourke, Andrew F G; Heard, Matthew S; Jordan, William C; Sumner, Seirian; Wang, Jinliang; Carvell, Claire

    2016-04-01

    Bumble bees (Bombus spp.) are important pollinators of both crops and wildflowers. Their contribution to this essential ecosystem service has been threatened over recent decades by changes in land use, which have led to declines in their populations. In order to design effective conservation measures, it is important to understand the effects of variation in landscape composition and structure on the foraging activities of worker bumble bees. This is because the viability of individual colonies is likely to be affected by the trade-off between the energetic costs of foraging over greater distances and the potential gains from access to additional resources. We used field surveys, molecular genetics, and fine resolution remote sensing to estimate the locations of wild bumble bee nests and to infer foraging distances across a 20-km² agricultural landscape in southern England, UK. We investigated five species, including the rare B. ruderatus and ecologically similar but widespread B. hortorum. We compared worker foraging distances between species and examined how variation in landscape composition and structure affected foraging distances at the colony level. Mean worker foraging distances differed significantly between species. Bombus terrestris, B. lapidarius, and B. ruderatus exhibited significantly greater mean foraging distances (551, 536, and 501 m, respectively) than B. hortorum and B. pascuorum (336 and 272 m, respectively). There was wide variation in worker foraging distances between colonies of the same species, which was in turn strongly influenced by the amount and spatial configuration of available foraging habitats. Shorter foraging distances were found for colonies where the local landscape had high coverage and low fragmentation of semi-natural vegetation, including managed agri-environmental field margins. The strength of relationships between different landscape variables and foraging distance varied between species, for example the strongest

  6. Phytochemical composition, antioxidant activity and HPLC fingerprinting profiles of three Pyrola species from different regions.

    PubMed

    Wang, Dongmei; He, Fengyuan; Lv, Zhenjiang; Li, Dengwu

    2014-01-01

    The present study was performed to investigate the variation of phytochemical composition, antioxidant activity and High Performance Liquid Chromatography (HPLC) fingerprinting profiles of three Pyrola species. Thirteen samples (eight P. decorata, three P. calliantha and two P. renifolia) were collected from different regions in China. The tannin, hyperoside and quercetin contents of all samples were determined by reverse-phase HPLC and varied within the range 9.77-34.75, 0.34-2.16 and 0.062-0.147 mg/g dry weigh, respectively. Total flavonoid content was evaluated and varied within the range 16.22-37.82 mg/g dry weight. Antioxidant activity was determined by DPPH assay, with IC50 ranging from 7.96 to 50.33 µg/ml, ABTS•+ and FRAP assay, within the range 612.66-1021.05 and 219.64-398.12 µmol equiv. Trolox/g, respectively. These results revealed that there were significant variations in phytochemical profiles and antioxidant activity among all samples. Due to the higher phytochemical content and significant antioxidant activity, P. calliantha was selected as the most valuable species, and the P. calliantha sample from Left banner of Alxa even possessed the strongest antioxidant activity among all the thirteen samples. Futhermore, Emei Mountain was proved to be the most suitable region for producing P. decorata. Moreover, in order to further evaluate the diversities and quality of Pyrola, HPLC fingerprint analysis coupled with hierarchical cluster and discrimination analyses were introduced to establish a simple, rapid and effective method for accurate identification, classification and quality assessment of Pyrola. Thirteen samples were divided into three groups consistent with their morphological classification. Two types of discriminant functions were generated and the ratio of discrimination was 100%. This method can identify different species of Pyrola and the same species from different regions of origin. Also, it can be used to compare and control the

  7. Phytochemical Composition, Antioxidant Activity and HPLC Fingerprinting Profiles of Three Pyrola Species from Different Regions

    PubMed Central

    Wang, Dongmei; He, Fengyuan; Lv, Zhenjiang; Li, Dengwu

    2014-01-01

    The present study was performed to investigate the variation of phytochemical composition, antioxidant activity and High Performance Liquid Chromatography (HPLC) fingerprinting profiles of three Pyrola species. Thirteen samples (eight P. decorata, three P. calliantha and two P. renifolia) were collected from different regions in China. The tannin, hyperoside and quercetin contents of all samples were determined by reverse-phase HPLC and varied within the range 9.77–34.75, 0.34–2.16 and 0.062–0.147 mg/g dry weigh, respectively. Total flavonoid content was evaluated and varied within the range 16.22–37.82 mg/g dry weight. Antioxidant activity was determined by DPPH assay, with IC50 ranging from 7.96 to 50.33 µg/ml, ABTS•+ and FRAP assay, within the range 612.66–1021.05 and 219.64–398.12 µmol equiv. Trolox/g, respectively. These results revealed that there were significant variations in phytochemical profiles and antioxidant activity among all samples. Due to the higher phytochemical content and significant antioxidant activity, P. calliantha was selected as the most valuable species, and the P. calliantha sample from Left banner of Alxa even possessed the strongest antioxidant activity among all the thirteen samples. Futhermore, Emei Mountain was proved to be the most suitable region for producing P. decorata. Moreover, in order to further evaluate the diversities and quality of Pyrola, HPLC fingerprint analysis coupled with hierarchical cluster and discrimination analyses were introduced to establish a simple, rapid and effective method for accurate identification, classification and quality assessment of Pyrola. Thirteen samples were divided into three groups consistent with their morphological classification. Two types of discriminant functions were generated and the ratio of discrimination was 100%. This method can identify different species of Pyrola and the same species from different regions of origin. Also, it can be used to compare and

  8. Allograft-prosthetic composite reverse total shoulder arthroplasty for reconstruction of proximal humerus tumor resections.

    PubMed

    King, Joseph J; Nystrom, Lukas M; Reimer, Nickolas B; Gibbs, C Parker; Scarborough, Mark T; Wright, Thomas W

    2016-01-01

    Proximal humerus reconstructions after resection of tumors are challenging. Early success of the reverse shoulder arthroplasty for reconstructions has recently been reported. The reverse allograft-prosthetic composite offers the advantage of improved glenohumeral stability compared with hemiarthroplasty for proximal humeral reconstructions as it uses the deltoid for stability. This article describes the technique for treating proximal humeral tumors, including preoperative planning, biopsy principles, resection pearls, soft tissue tensioning, and specifics about reconstruction using the reverse allograft-prosthetic composite. Two cases are presented along with the functional outcomes with use of this technique. Biomechanical considerations during reconstruction are reviewed, including techniques to improve the deltoid compression force. Reported instability rates are less with reverse shoulder arthroplasty reconstruction as opposed to hemiarthroplasty or total shoulder arthroplasty reconstructions of tumor resections. Reported functional outcomes are promising for the reverse allograft-prosthetic composite reconstructions, although complications are reported. Reverse allograft-prosthetic composites are a promising option for proximal humeral reconstructions, although nonunion of the allograft-host bone junction continues to be a challenge for this technique. Copyright © 2016 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.

  9. Comparison of species composition and richness of fish assemblages in altered and unaltered littoral habitats

    USGS Publications Warehouse

    Poe, T.P.; Hatcher, C.O.; Brown, C.L.; Schloesser, D.W.

    1986-01-01

    Species composition and richness of fish assemblages in altered and unaltered littoral habitats in Lake St. Clair, Michigan, differed between areas. A percid-cyprinid-cyprinodontid assemblage dominated in the unaltered area, Muscamoot Bay, which has a natural shoreline (with almost no alteration due to dredging or bulkheading), high water quality, and high species richness of aquatic macrophytes. A centrarchid assemblage dominated in the altered area, Belvidere Bay, which has a bulkheaded shoreline, many dredged areas, reduced water quality due to inputs of nutrients from a nearby river, and relatively low species richness of aquatic macrophytes. Habitat factors, species richness and abundance of aquatic macrophytes, had the most influence on fish community structure in both areas. The percid-cyprinid-cyprinodontid assemblage was significantly correlated with six species of macrophytes whereas the centrarchid assemblage was significantly correlated with only four. These patterns suggest that preference for diverse habitats was higher, and tolerance to habitat alteration lower, in percid-cyprinid-cyprinodontid assemblages than in centrarchid assemblages.

  10. Chemical Composition and Acetylcholinesterase Inhibitory Activity of Essential Oils from Piper Species.

    PubMed

    Xiang, Cai-Peng; Han, Jia-Xin; Li, Xing-Cong; Li, Yun-Hui; Zhang, Yi; Chen, Lin; Qu, Yan; Hao, Chao-Yun; Li, Hai-Zhou; Yang, Chong-Ren; Zhao, San-Jun; Xu, Min

    2017-05-10

    The essential oils (EOs) derived from aromatic plants such as Piper species are considered to play a role in alleviating neuronal ailments that are associated with inhibition of acetylcholinesterase (AChE). The chemical compositions of 23 EOs prepared from 16 Piper spp. were analyzed by both gas chromatography with a flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). A total of 76 compounds were identified in the EOs from the leaves and stems of 19 samples, while 30 compounds were detected in the EOs from the fruits of four samples. Sesquiterpenes and phenylpropanoids were found to be rich in these EOs, of which asaricin, caryophyllene, caryophyllene oxide, isospathulenol, (+)-spathulenol, and β-bisabolene are the major constituents. The EOs from the leaves and stems of Piper austrosinense, P. puberulum, P. flaviflorum, P. betle, and P. hispidimervium showed strong AChE inhibitory activity with IC 50 values in the range of 1.51 to 13.9 mg/mL. A thin-layer chromatography (TLC) bioautography assay was employed to identify active compound(s) in the most active EO from P. hispidimervium. The active compound was isolated and identified as asaricin, which gave an IC 50 value of 0.44 ± 0.02 mg/mL against AChE, comparable to galantamine with an IC 50 0.15 ± 0.01 mg/mL.

  11. Seventy-year record of changes in the composition of overstory species by elevation on the Bartlett Experimental Forest

    Treesearch

    William B. Leak; Mariko Yamasaki

    2010-01-01

    Remeasurements over a 70-year period (1931-1932 to 2002-2003) on 404 cruise plots on the Bartlett Experimental Forest in New Hampshire provided a record of landscape-level changes in the composition of overstory species over time by elevation and d.b.h. (diameter at breast height) classes. Typically, early to midsuccessional species declined while late successional...

  12. Composition of essential oils in subterranean organs of three species of Valeriana L.

    PubMed

    Samaneh, Ekhteraei Tousi; Tayebeh, Radjabian; Hassan, Ebrahimzadeh; Vahid, Niknam

    2010-11-01

    Essential oils from the subterranean organs of three species of Valeriana L. from Iran (Valeriana sisymbriifolia Vahl, Valeriana alliariifolia Adams and Valeriana officinalis L.) belonging to Valerianaceae family have been obtained by hydrodistillation and analysed by gas chromatography-mass spectrometry in order to discern the differences and similarities between the volatile chemical compositions of these species. More than 100 components were identified in essential oils of the studied plants (Supplementary Table S1--online only). The principal common constituents of the three species of Valeriana were spathulenol, limonene, γ-terpinene, vulgarone B and p-cymene. The main essential oil ingredients were α-selinene (7.83%) in V. sisymbriifolia, limonene (3.53%) in V. alliariifolia and spathulenol (13.33%), α-campholenal (11.48%), vulgarone B (8.38%) and valerenal (8.32%) in V. officinalis plants. Ageratochromene (precocene II), a chromene substance with antibacterial, antifungal, insecticidal and antijuvenile hormonal activities, was found at high levels (35.59% and 36.58%) in the essential oils of V. sisymbriifolia plants.

  13. Characterisation of larval habitats, species composition and factors associated with the seasonal abundance of mosquito fauna in Gezira, Sudan.

    PubMed

    Mahgoub, Mostafa M; Kweka, Eliningaya J; Himeidan, Yousif E

    2017-02-08

    Larval source management (LSM), which requires an understanding of the ecology and composition of the local mosquito fauna, is an important parameter in successful vector control programmes. The present study was conducted to understand the distribution of larval habitats, species composition and factors associated with the seasonal abundance of mosquito larvae in Gezira irrigation Scheme in Gezira state, central Sudan. Cross-sectional larval surveys were carried out in the communities of Barakat (urban) and El-Kareiba (semi-urban), in Wad Madani, Gezira. A standard dipper was used for sampling larvae in all possible breeding sites and enamel bowls were employed for larvae sorting. Habitats were characterised using physical features and all larvae specimens were identified morphologically. A total of 331 larval habitats were surveyed, out of which 166 were found to be positive breeding sites for Anopheles (56.78%), Culicinae (29.67%) and Aedes (13.55%) species. A total of 5 525 larvae collected were categorised as Culex (2 617, 47.37%), Anopheles (2 600, 47.06%) and Aedes (308, 5.57%). There was a high number of positive habitats during the rainy season, while the lowest proportion was reported during the hot dry season, in both study sites (Barakat [χ 2  = 10.641, P = 0.0090], El-Kareiba [χ 2  = 23.765, P = 0.0001]). The main breeding site for Anopheles larvae was leaking water pipes (51.5%), followed by irrigation channels (34.2%), hoof prints (6.4%), tyre tracks (5.5%) and water tanks (2.4%). A logistic regression analysis showed that the abundance of Anopheles larvae was reduced by the presence of predators (backswimmers, tadpoles) and grass cover. Adult productivity (number of adult females emerged/m 2 ) was not homogeneousfor all habitats; the highest productivity was found in irrigation channels (0.78 females/m 2 ) for Anopheles, and in septic tanks (2.86 females/m 2 ) for Culicinae and (0.86 females/m 2 ) for Aedes. Anopheles arabiensis

  14. Tree Species Composition and Harvest Intensity Affect Herbivore Density and Leaf Damage on Beech, Fagus sylvatica, in Different Landscape Contexts

    PubMed Central

    Mangels, Jule; Blüthgen, Nico; Frank, Kevin; Grassein, Fabrice; Hilpert, Andrea; Mody, Karsten

    2015-01-01

    Most forests are exposed to anthropogenic management activities that affect tree species composition and natural ecosystem processes. Changes in ecosystem processes such as herbivory depend on management intensity, and on regional environmental conditions and species pools. Whereas influences of specific forest management measures have already been addressed for different herbivore taxa on a local scale, studies considering effects of different aspects of forest management across different regions are rare. We assessed the influence of tree species composition and intensity of harvesting activities on arthropod herbivores and herbivore-related damage to beech trees, Fagus sylvatica, in 48 forest plots in three regions of Germany. We found that herbivore abundance and damage to beech trees differed between regions and that – despite the regional differences - density of tree-associated arthropod taxa and herbivore damage were consistently affected by tree species composition and harvest intensity. Specifically, overall herbivore damage to beech trees increased with increasing dominance of beech trees – suggesting the action of associational resistance processes – and decreased with harvest intensity. The density of leaf chewers and mines was positively related to leaf damage, and several arthropod groups responded to beech dominance and harvest intensity. The distribution of damage patterns was consistent with a vertical shift of herbivores to higher crown layers during the season and with higher beech dominance. By linking quantitative data on arthropod herbivore abundance and herbivory with tree species composition and harvesting activity in a wide variety of beech forests, our study helps to better understand the influence of forest management on interactions between a naturally dominant deciduous forest tree and arthropod herbivores. PMID:25938417

  15. Species-specific accumulation of methyl and total mercury in sharks from offshore and coastal waters of Korea.

    PubMed

    Kim, Sang-Jo; Lee, Hyun-Kyung; Badejo, Abimbola C; Lee, Won-Chan; Moon, Hyo-Bang

    2016-01-15

    Limited information is available on mercury (Hg) levels in various shark species consumed in Korea. The methyl-Hg (Me-Hg) and total Hg concentrations in all shark species ranged from 0.08 to 4.5 (mean: 1.2) mg/kg wet weight and from 0.1 to 7.0 (mean: 1.4) mg/kg wet weight, respectively. Inter-species differences in Hg accumulation were found among the species; however, Hg accumulation was homogenous between dorsal and pectoral fins within species. The highest Hg levels were found in aggressive carnivore shark species. Trophic position was important in determining Hg accumulation for aggressive carnivore sharks. Approximately 80% of shark species exceeded the safety limits for Me-Hg established by domestic and international authorities. The mean estimated daily intake of Me-Hg (1.3 μg/kg body weight/day) for Korean populations consuming various sharks was higher than the guidelines proposed by international regulatory authorities, suggesting that excessive shark fin consumption may pose potential health risks for Koreans. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. Total petroleum systems of the Pelagian Province, Tunisia, Libya, Italy, and Malta; the Bou Dabbous, Tertiary and Jurassic-Cretaceous composite

    USGS Publications Warehouse

    Klett, T.R.

    2001-01-01

    Undiscovered conventional oil and gas resources were assessed within total petroleum systems of the Pelagian Province (2048) as part of the U.S. Geological Survey World Petroleum Assessment 2000. The Pelagian Province is located mainly in eastern Tunisia and northwestern Libya. Small portions of the province extend into Malta and offshore Italy. Although several petroleum systems may exist, only two ?composite? total petroleum systems were identified. Each total petroleum system comprises a single assessment unit. These total petroleum systems are called the Bou Dabbous?Tertiary and Jurassic-Cretaceous Composite, named after the source-rock intervals and reservoir-rock ages. The main source rocks include mudstone of the Eocene Bou Dabbous Formation; Cretaceous Bahloul, Lower Fahdene, and M?Cherga Formations; and Jurassic Nara Formation. Known reservoirs are in carbonate rocks and sandstone intervals throughout the Upper Jurassic, Cretaceous, and Tertiary sections. Traps for known accumulations include fault blocks, low-amplitude anticlines, high-amplitude anticlines associated with reverse faults, wrench fault structures, and stratigraphic traps. The estimated means of the undiscovered conventional petroleum volumes in total petroleum systems of the Pelagian Province are as follows: [MMBO, million barrels of oil; BCFG, billion cubic feet of gas; MMBNGL, million barrels of natural gas liquids] Total Petroleum System MMBO BCFG MMBNGL Bou Dabbous?Tertiary 667 2,746 64 Jurassic-Cretaceous Composite 403 2,280 27

  17. Detecting the role of individual species for overyielding in experimental grassland communities composed of potentially dominant species.

    PubMed

    Roscher, Christiane; Schumacher, Jens; Weisser, Wolfgang W; Schmid, Bernhard; Schulze, Ernst-Detlef

    2007-12-01

    Several studies have shown that the contribution of individual species to the positive relationship between species richness and community biomass production cannot be easily predicted from species monocultures. Here, we used a biodiversity experiment with a pool of nine potentially dominant grassland species to relate the species richness-productivity relationship to responses in density, size and aboveground allocation patterns of individual species. Aboveground community biomass increased strongly with the transition from monocultures to two-species mixtures but only slightly with the transition from two- to nine-species mixtures. Tripartite partitioning showed that the strong increase shown by the former was due to trait-independent complementarity effects, while the slight increase shown by the latter was due to dominance effects. Trait-dependent complementarity effects depended on species composition. Relative yield total (RYT) was greater than 1 (RYT>1) in mixtures but did not increase with species richness, which is consistent with the constant complementarity effect. The relative yield (RY) of only one species, Arrhenatherum elatius, continually increased with species richness, while those of the other species studied decreased with species richness or varied among different species compositions within richness levels. High observed/expected RYs (RYo/RYe>1) of individual species were mainly due to increased module densities, whereas low observed/expected RYs (RYo/RYe<1) were due to more pronounced decreases in module density (species with stoloniferous or creeping growth) or module size (species with clearly-defined plant individuals). The trade-off between module density and size, typical for plant populations under the law of constant final yield, was compensated among species. The positive trait-independent complementarity effect could be explained by an increase in community module density, which reached a maximum at low species richness. In contrast

  18. Rapid determination of Swiss cheese composition by Fourier transform infrared/attenuated total reflectance spectroscopy.

    PubMed

    Rodriguez-Saona, L E; Koca, N; Harper, W J; Alvarez, V B

    2006-05-01

    There is a need for rapid and simple techniques that can be used to predict the quality of cheese. The aim of this research was to develop a simple and rapid screening tool for monitoring Swiss cheese composition by using Fourier transform infrared spectroscopy. Twenty Swiss cheese samples from different manufacturers and degree of maturity were evaluated. Direct measurements of Swiss cheese slices (approximately 0.5 g) were made using a MIRacle 3-reflection diamond attenuated total reflectance (ATR) accessory. Reference methods for moisture (vacuum oven), protein content (Kjeldahl), and fat (Babcock) were used. Calibration models were developed based on a cross-validated (leave-one-out approach) partial least squares regression. The information-rich infrared spectral range for Swiss cheese samples was from 3,000 to 2,800 cm(-1) and 1,800 to 900 cm(-1). The performance statistics for cross-validated models gave estimates for standard error of cross-validation of 0.45, 0.25, and 0.21% for moisture, protein, and fat respectively, and correlation coefficients r > 0.96. Furthermore, the ATR infrared protocol allowed for the classification of cheeses according to manufacturer and aging based on unique spectral information, especially of carbonyl groups, probably due to their distinctive lipid composition. Attenuated total reflectance infrared spectroscopy allowed for the rapid (approximately 3-min analysis time) and accurate analysis of the composition of Swiss cheese. This technique could contribute to the development of simple and rapid protocols for monitoring complex biochemical changes, and predicting the final quality of the cheese.

  19. Flow regime in a restored wetland determines trophic links and species composition in the aquatic macroinvertebrate community.

    PubMed

    González-Ortegón, E; Walton, M E M; Moghaddam, B; Vilas, C; Prieto, A; Kennedy, H A; Pedro Cañavate, J; Le Vay, L

    2015-01-15

    In a restored wetland (South of Spain), where different flow regimes control water exchange with the adjacent Guadalquivir estuary, the native Palaemon varians coexists with an exotic counterpart species Palaemon macrodactylus. This controlled m\\acrocosm offers an excellent opportunity to investigate how the effects of water management, through different flow regimes, and the presence of a non-native species affect the aquatic community and the trophic niche (by gut contents and C-N isotopic composition) of the native shrimp Palaemon varians. We found that increased water exchange rate (5% day(-1) in mixed ponds vs. 0.1% day(-1) in extensive ponds) modified the aquatic community of this wetland; while extensive ponds are dominated by isopods and amphipods with low presence of P. macrodactylus, mixed ponds presented high biomass of mysids, corixids, copepods and both shrimp species. An estuarine origin of nutrients and primary production might explain seasonal and spatial differences found among ponds of this wetland. A combined analysis of gut contents and isotopic composition of the native and the exotic species showed that: (1) native P. varians is mainly omnivorous (2) while the non-native P. macrodactylus is more zooplanktivorous and (3) a dietary overlap occurred when both species coexist at mixed ponds where a higher water exchange and high abundance of mysids and copepods diversifies the native species' diet. Thus differences in the trophic ecology of both species are clearly explained by water management. This experimental study is a valuable tool for integrated management between river basin and wetlands since it allows quantification of wetland community changes in response to the flow regime. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Total Bee Dependence on One Flower Species Despite Available Congeners of Similar Floral Shape

    PubMed Central

    González-Varo, Juan P.; Ortiz-Sánchez, F. Javier; Vilà, Montserrat

    2016-01-01

    Extreme specialization is a common phenomenon in antagonistic biotic interactions but it is quite rare in mutualistic ones. Indeed, bee specialization on a single flower species (monolecty) is a questioned fact. Here, we provide multiple lines of evidence on true monolecty in a solitary bee (Flavipanurgus venustus, Andrenidae), which is consistent across space (18 sites in SW Iberian Peninsula) and time (three years) despite the presence of closely related congeneric plant species whose flowers are morphologically similar. The host flower (Cistus crispus, Cistaceae) is in turn a supergeneralist, visited by at least 85 insect species. We uncover ultraviolet light reflectance as a distinctive visual cue of the host flower, which can be a key mechanism because bee specialization has an innate basis to recognize specific signals. Moreover, we hypothesized that a total dependence on an ephemeral resource (i.e. one flower species) must lead to spatiotemporal matching with it. Accordingly, we prove that the bee’s flight phenology is synchronized with the blooming period of the host flower, and that the densities of bee populations mirror the local densities of the host flower. This case supports the ‘predictable plethora’ hypothesis, that is, that host-specialization in bees is fostered by plant species providing predictably abundant floral resources. Our findings, along with available phylogenetic information on the genus Cistus, suggest the importance of historical processes and cognitive constraints as drivers of specialization in bee-plant interactions. PMID:27658205

  1. Species composition, co-occurrence, association and affinity indices of mosquito larvae (Diptera: Culicidae) in Mazandaran Province, northern Iran.

    PubMed

    Nikookar, Seyed Hassan; Azari-Hamidian, Shahyad; Fazeli-Dinan, Mahmoud; Nasab, Seyed Nouraddin Mousavi; Aarabi, Mohsen; Ziapour, Seyyed Payman; Enayati, Ahmadali

    2016-05-01

    Although considerable progress has been made in the past years in management of mosquito borne diseases such as malaria, dengue, yellow fever and West Nile fever through research in biology and ecology of the vectors, these diseases are still major threats to human health. Therefore, more research is required for better management of the diseases. This investigation provides information on the composition, co-occurrence, association and affinity indices of mosquito larvae in Mazandaran Province, northern Iran. In a large scale field study, mosquito larvae were collected from 120 sentinel sites in 16 counties in Mazandaran Province, using standard 350 ml dipper. Sampling took place monthly from May to December 2014. Collected larvae were mounted on glass slides using de Faure's medium and were diagnosed using morphological characters. Totally, 19,840 larvae were collected including three genera and 16 species from 120 larval habitats, as follows: Anopheles claviger, Anopheles hyrcanus, Anopheles maculipennis s.l., Anopheles marteri, Anopheles plumbeus, Anopheles pseudopictus, Culex pipiens, Culex tritaeniorhynchus, Culex torrentium, Culex perexiguus, Culex territans, Culex mimeticus, Culex hortensis, Culiseta annulata, Culiseta longiareolata, and Culiseta morsitans. Predominant species were Cx. pipiens and An. maculipennis s.l. which show the highest co-occurrence. The pair of species An. hyrcanus/An. pseudopictus showed significant affinity and association. High co-occurrence of the predominant species Cx. pipiens and An. maculipennis s.l. in the study area is of considerable importance in terms of vector ecology. It was also revealed that An. pseudopictus/An. hyrcanus often occur sympatrically indicating their common habitat requirements. The information may be equally important when vector control measures are considered. Copyright © 2016 Elsevier B.V. All rights reserved.

  2. Species distribution modelling for plant communities: Stacked single species or multivariate modelling approaches?

    Treesearch

    Emilie B. Henderson; Janet L. Ohmann; Matthew J. Gregory; Heather M. Roberts; Harold S.J. Zald

    2014-01-01

    Landscape management and conservation planning require maps of vegetation composition and structure over large regions. Species distribution models (SDMs) are often used for individual species, but projects mapping multiple species are rarer. We compare maps of plant community composition assembled by stacking results from many SDMs with multivariate maps constructed...

  3. Essential oil composition from two species of Piperaceae family grown in Colombia.

    PubMed

    Pino Benitez, Nayive; Meléndez León, Erika M; Stashenko, Elena E

    2009-10-01

    Essential oil compositions of aerial parts from two species in the Piper (Piperaceae family) genera: Piper lanceaefolium Kunth and Piper hispidum Sw., frequently called deflated (for the anti-inflammatory activity) or cord. Piperaceae leaves were collected in different regions of the Chocó department in northwestern Colombia and identified by botanists from Colombian National Herbarium, where a voucher of each specimen were deposited (No- COL 519993 and No- COL 519969, respectively). The essential oils were obtained by microwave-assisted hydrodistillation (MWHD) and analyzed by gas chromatography-mass spectrometry (GC-MS). The P. lanceaefolium essential oil was sesquiterpenoid type (71.7%). This composition was represented by sesquiterpenes hydrocarbons (58.5%) and by their oxygenated derivates (13.2%); the main compounds were, trans-beta-caryophyllene (11.6%) and germacrene D (10.7%) followed by alpha-selinene (7.8%), beta-pinene (5.4%), beta-selinene (4.8%), and alpha-cubebene (4.3%). The Piper hispidum essential oil also was sesquiterpene type (74.4%) and oxygenated sesquiterpenes (46.4%) followed by sesquiterpenes hydrocarbons (28.0%). The main compounds were trans-nerolidol (23.6%) and caryophyllene oxide (5.4%) followed by beta-elemene (5.1%), trans-beta-caryophyllene (5.1%), curzerene (4.9%), and germacrene B (4.5%). Trans-beta-caryophyllene presents the higher percentage of the common compounds in the two species' essential oil (11.6% and 5.1% in P. lanceaefolium and P. hispidum, respectively).

  4. Bumble bees (Hymenoptera: Apidae: Bombus spp.) of interior Alaska: Species composition, distribution, seasonal biology, and parasites

    USDA-ARS?s Scientific Manuscript database

    Despite the ecological and agricultural significance of bumble bees in Alaska, very little is known and published about this important group at the regional level. The objectives of this study were to provide baseline data on species composition, distribution, seasonal biology, and parasites of the ...

  5. Total selenium and selenium species in irrigation drain inflows to the Salton Sea, California, October 2007 and January 2008

    USGS Publications Warehouse

    May, Thomas W.; Walther, Michael J.; Saiki, Michael K.; Brumbaugh, William G.

    2008-01-01

    This report presents the results for two sampling periods (October 2007 and January 2008) during a 4-year monitoring program to characterize selenium concentrations in selected irrigation drains flowing into the Salton Sea, California. Total selenium, selenium species (selenite, selenate, organoselenium), and total suspended solids were determined in water samples, and total selenium was determined in sediment, detritus, and biota that included algae, plankton, midge larvae (family, Chironomidae), and two fish species?western mosquitofish (Gambusia affinis) and sailfin molly (Poecilia latipinna). In addition, sediments were analyzed for percent total organic carbon and particle size. Mean total selenium concentrations in water for both sampling periods ranged from 0.97 to 64.5 micrograms per liter, predominately as selenate, which is typical of waters where selenium is leached out of selenium-containing marine shales and associated soils under alkaline and oxidizing conditions. Total selenium concentrations (micrograms per gram dry weight) ranged as follows: algae, 0.95 to 5.99; plankton, 0.15 to 19.3; midges, 1.39 to 15.4; fish, 3.71 to 25.1; detritus, 0.85 to 21.7; sediment, 0.32 to 7.28.

  6. Total selenium and selenium species in irrigation drain inflows to the Salton Sea, California, October 2008 and January 2009

    USGS Publications Warehouse

    May, Thomas W.; Walther, Michael J.; Saiki, Michael K.; Brumbaugh, William G.

    2009-01-01

    This report presents the results for two sampling periods (October 2008 and January 2009) during a 4-year monitoring program to characterize selenium concentrations in selected irrigation drains flowing into the Salton Sea, California. Total selenium, selenium species (dissolved selenite, selenate, organoselenium), and total suspended solids were determined in water samples. Total selenium also was determined in water column particulates and in sediment, detritus, and biota that included algae, plankton, midge larvae (family, Chironomidae), and two fish species (western mosquitofish, Gambusia affinis, and sailfin molly, Poecilia latipinna). In addition, sediments were analyzed for percent total organic carbon and particle size. Mean total selenium concentrations in water for both sampling periods ranged from 1.00 to 33.6 micrograms per liter, predominately as selenate, which is typical of waters where selenium is leached out of selenium-containing marine shales and associated soils under alkaline and oxidizing conditions. Total selenium concentrations (micrograms per gram dry weight) ranged as follows: algae, 1.52 to 8.26; plankton, 0.79 to 3.66; midges, 2.68 to 50.6; fish, 3.09 to 30.4; detritus, 1.78 to 58.0; and sediment, 0.42 to 10.0.

  7. Antioxidant and anticholinesterase activities of five wild mushroom species with total bioactive contents.

    PubMed

    Tel, Gulsen; Ozturk, Mehmet; Duru, Mehmet E; Turkoglu, Aziz

    2015-06-01

    Recently, mushrooms are interesting natural products to be investigated due to exhibiting various bioactivities. This study determines the antioxidant and anticholinesterase activities of various extracts of five wild mushroom species. In addition, the total bioactive contents, namely, ascorbic acid, β-carotene, and lycopene along with phenolic and flavonoid contents were also determined spectrophotometrically. Antioxidant activity was tested by using five complementary tests; namely, β-carotene-linoleic acid, DPPH(•) scavenging, ABTS(•+) scavenging, cupric-reducing antioxidant capacity (CUPRAC), and metal chelating assays. The in vitro anticholinesterase activity was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes using the Ellman method. The spectrophotometric methods were used to determine the total phenolic, flavonoid, ascorbic acid, β-carotene, and lycopene contents. The current study has shown that ethyl acetate extracts of Ganoderma lucidum (Curtis) P. Karst (IC50: 1.55 ± 0.05 µg/mL) and Funalia trogii (Berk.) Bondartsev & Singer (IC50: 4.31 ± 0.18 µg/mL) exhibited good lipid peroxidation inhibitory activity. The DPPH, ABTS, and CUPRAC assays supported this activity. The ethyl acetate and methanol extracts of Funalia trogii and Ganoderma lucidum indicated good anticholinesterase activity. Ganoderma lucidum had rich phenolic and flavonoid contents, indicating 98.67 ± 0.32 mg PEs/g extract and 160.38 ± 1.25 mg QEs/g extract, respectively. The results demonstrate that some of the mushroom species tested herein could be used in food and pharmaceutical industries as natural antioxidants.

  8. MECHANICAL PROPERTIES OF TOTALLY PERMEABLE TITANIUM COMPOSITE PYLON FOR DIRECT SKELETAL ATTACHMENT

    PubMed Central

    Pitkin, M.; Pilling, J.; Raykhtsaum, G.

    2012-01-01

    Composite pylons containing a solid titanium core with drilled holes surrounded by a porous sintered titanium shell have been fabricated and tested in bending along with the raw cores and pylons composed of the porous titanium alone. The new pylons were designed with the concept of enhanced ingrowth of bone and skin cells and are intended for direct skeletal attachment of limb prostheses considering requirements for long-lasting anchorage to the residuum bone and a need for a safe skin-implant seal. Load-displacement thresholds were determined after which the integrity of the porous component may be compromised. The composite pylons have a flexural strength and stiffness substantially greater than that of pylons composed of the porous titanium alone. The drilled holes in the solid insert have been shown to have virtually no effect on the flexural strength of the pylon, while meeting a requirement for total permeability of the device for unrestricted cell ingrowth. The predicted strength of the pylons and associated failure modes are in close agreement with those measured. PMID:22287509

  9. Species Composition and Abundance of Stink Bugs (Hemiptera: Heteroptera: Pentatomidae) in Minnesota Field Corn.

    PubMed

    Koch, Robert L; Pahs, Tiffany

    2015-04-01

    In response to concerns of increasing significance of stink bugs (Hemiptera: Heteroptera: Pentatomidae) in northern states, a survey was conducted over 2 yr in Minnesota to characterize the Pentatomidae associated with field corn, Zea mays L. Halyomorpha halys (Stål), an exotic species, was not detected in this survey, despite continued detection of this species as an invader of human-made structures in Minnesota. Five species of Pentatomidae (four herbivorous; one predatory) were collected from corn. Across years, Euschistus variolarius (Palisot de Beauvois) and Euschistus servus euschistoides (Vollenhoven) had the greatest relative abundances and frequencies of detection. In 2012, the abundance of herbivorous species exceeded 25 nymphs and adults per 100 plants (i.e., an economic threshold) in 0.48% of fields. However, the abundance of herbivorous species did not reach economic levels in any fields sampled in 2013. The frequency of detection of herbivorous species and ratio of nymphs to adults was highest during reproductive growth stages of corn. The predator species, Podisus maculiventris (Say), was detected in 0 to 0.32% of fields. These results provide baseline information on the species composition and abundance of Pentatomidae in Minnesota field corn, which will be necessary for documentation of changes to this fauna as a result of the invasion of H. halys and to determine if some native species continue to increase in abundance in field crops. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  10. Patterns of plant diversity loss and species turnover resulting from land abandonment and intensification in semi-natural grasslands.

    PubMed

    Uchida, Kei; Koyanagi, Tomoyo F; Matsumura, Toshikazu; Koyama, Asuka

    2018-07-15

    Land-use changes cause biodiversity loss in semi-natural ecosystems worldwide. Biotic homogenization has led to biodiversity loss, mainly through declines in species composition turnover. Elucidating patterns of turnover in species composition could enhance our understanding of how anthropogenic activities affect community assembly. Here, we focused on whether the decreasing patterns in plant diversity and turnover of species composition resulting from land-use change vary in two regions. We estimated the species diversity and composition of semi-natural grasslands surrounding paddy fields in satoyama landscapes. We examined the differences in species diversity and composition across three land-use types (abandoned, traditional, and intensified) in two regions (Hyogo and Niigata Prefectures, Japan), which were characterized by different climatic conditions. We then assessed alpha-, beta-, and gamma-diversity to compare the patterns of diversity losses in the two regions as a result of land-use changes. In each region, gamma-diversity was consistently higher in the traditional sites compared to abandoned or intensified sites. The analyses revealed that most of the beta-diversity in traditional sites differed significantly from those of abandoned and intensified sites in both regions. However, the beta-diversity of total and perennial species did not differ between traditional and abandoned sites in the Hyogo region. We noted that the beta-diversity of total and perennial species in intensified sites was much lower than that in the traditional sites of the Niigata region. Overall, the patterns of alpha- and gamma-diversity loss were similar in both study regions. Although the biotic homogenization was caused by intensified land-use in the Niigata region, this hypothesis did not completely explain the loss of biodiversity in the abandoned sites in the Hyogo region. The present study contributes to the growing body of work investigating changes in biodiversity as a

  11. Comparison of lipid content and Fatty Acid composition in the edible meat of wild and cultured freshwater and marine fish and shrimps from china.

    PubMed

    Li, Guipu; Sinclair, Andrew J; Li, Duo

    2011-03-09

    The lipid content and fatty acid composition in the edible meat of twenty-nine species of wild and cultured freshwater and marine fish and shrimps were investigated. Both the lipid content and fatty acid composition of the species were specified due to their unique food habits and trophic levels. Most of the marine fish demonstrated higher lipid content than the freshwater fish, whereas shrimps had the lowest lipid content. All the marine fish and shrimps had much higher total n-3 PUFA than n-6 PUFA, while most of the freshwater fish and shrimps demonstrated much lower total n-3 PUFA than n-6 PUFA. This may be the biggest difference in fatty acid composition between marine and freshwater species. The cultured freshwater fish demonstrated higher percentages of total PUFA, total n-3 PUFA, and EPA + DHA than the wild freshwater fish. Two freshwater fish, including bighead carp and silver carp, are comparable to the marine fish as sources of n-3 PUFA.

  12. Comparison of species composition and fumonisin production in Aspergillus section Nigri populations in maize kernels from USA and Italy.

    PubMed

    Susca, Antonia; Moretti, Antonio; Stea, Gaetano; Villani, Alessandra; Haidukowski, Miriam; Logrieco, Antonio; Munkvold, Gary

    2014-10-01

    Fumonisin contamination of maize is considered a serious problem in most maize-growing regions of the world, due to the widespread occurrence of these mycotoxins and their association with toxicosis in livestock and humans. Fumonisins are produced primarily by species of Fusarium that are common in maize grain, but also by some species of Aspergillus sect. Nigri, which can also occur on maize kernels as opportunistic pathogens. Understanding the origin of fumonisin contamination in maize is a key component in developing effective management strategies. Although some fungi in Aspergillus sect. Nigri are known to produce fumonisins, little is known about the species which are common in maize and whether they make a measurable contribution to fumonisin contamination of maize grain. In this work, we evaluated populations of Aspergillus sect. Nigri isolated from maize in USA and Italy, focusing on analysis of housekeeping genes, the fum8 gene and in vitro capability of producing fumonisins. DNA sequencing was used to identify Aspergillus strains belonging to sect. Nigri, in order to compare species composition between the two populations, which might influence specific mycotoxicological risks. Combined beta-tubulin/calmodulin sequences were used to genetically characterize 300 strains (199 from Italy and 101 from USA) which grouped into 4 clades: Aspergillus welwitschiae (syn. Aspergillus awamori, 14.7%), Aspergillus tubingensis (37.0%) and Aspergillus niger group 1 (6.7%) and group 2 (41.3%). Only one strain was identified as Aspergillus carbonarius. Species composition differed between the two populations; A. niger predominated among the USA isolates (69%), but comprised a smaller percentage (38%) of Italian isolates. Conversely, A. tubingensis and A. welwitschiae occurred at higher frequencies in the Italian population (42% and 20%, respectively) than in the USA population (27% and 5%). The evaluation of FB2 production on CY20S agar revealed 118 FB2 producing and 84

  13. Seed oil and fatty acid composition in Capsicum spp

    USDA-ARS?s Scientific Manuscript database

    The oil content and fatty acid composition of seed of 233 genebank accessions (total) of nine Capsicum species, and a single accession of Tubocapsicum anomalum, were determined. The physicochemical characteristics of oil extracted from seed of C. annuum and C. baccatum were also examined. Significan...

  14. Helminth communities of four commercially important fish species from Chetumal Bay, Mexico.

    PubMed

    Aguirre-Macedo, M L; Vidal-Martínez, V M; González-Solís, D; Caballero, P I

    2007-03-01

    The relative importance of ecology and evolution as factors determining species richness and composition of the helminth communities of fish is a matter of current debate. Theoretical studies use host-parasite lists, but these do not include studies on a temporal or spatial scale. Local environmental conditions and host biological characteristics are shown to influence helminth species richness and composition in four fish species (Eugerres plumieri, Hexanematichthys assimilis, Oligoplites saurus, and Scomberomorus maculatus) in Chetumal Bay, Mexico. With the exception of H. assimilis, the helminth communities had not been previously studied and possible associations between environmental and host biological characteristics as factors determining helminth species richness and composition using redundancy analysis (RDA) are described. Thirty-four helminth species are identified, with the highest number of species (19 total (mean = 6.3 +/- 2.1)) and the lowest (9 (4.0 +/- 1.0)) occurring in H. assimilis and S. maculatus, respectively. The larval nematodes Contracaecum sp. and Pseudoterranova sp. were not only the helminth species shared by all four host species but also were the most prevalent and abundant. Statistical associations between helminth community parameters and local ecological variables such as host habitat use, feeding habits, mobility, and time of residence in coastal lagoons are identified. Phylogeny is important because it clearly separates all four host species by their specialist parasites, although specific habitat and feeding habits also significantly influence the differentiation between the four fish species.

  15. Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.

    PubMed

    Peng, Xiaowei; Li, Chao; Liu, Jing; Yi, Zili; Han, Yejun

    2017-07-01

    The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season. For all the four species, there was only a slight change in the compositional content. Meanwhile there was a huge change in the BMP values. At the growth time of 60days the BMPs ranged from 247.1 to 266.5mlg -1 VS. As growth time was prolonged, the BMPs decreased by 11-35%. For each species, the BMP was positively correlated to the cellulose degradability with the correlation coefficients (R 2 ) ranging from 0.8055 to 0.9925. This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species.

    PubMed

    Gursoy, Nevcihan; Sarikurkcu, Cengiz; Cengiz, Mustafa; Solak, M Halil

    2009-09-01

    Seven Morchella species were analyzed for their antioxidant activities in different test systems namely beta-carotene/linoleic acid, DPPH, reducing power, chelating effect and scavenging effect (%) on the stable ABTS*(+), in addition to their heavy metals, total phenolic and flavonoid contents. In beta-carotene/linoleic acid system, the most active mushrooms were M. esculenta var. umbrina and M.angusticeps. In the case of DPPH, methanol extract of M. conica showed high antioxidant activity. The reducing power of the methanol extracts of mushrooms increased with concentration. Chelating capacity of the extracts was also increased with the concentration. On the other hand, in 40 microg ml(-1) concentration, methanol extract of M. conica, exhibited the highest radical scavenging activity (78.66+/-2.07%) when reacted with the ABTS*(+) radical. Amounts of seven elements (Cu, Mn, Co, Zn, Fe, Ca, and Mg) and five heavy metals (Ni, Pb, Cd, Cr, and Al) were also determined in all species. M. conica was found to have the highest phenolic content among the samples. Flavonoid content of M. rotunda was also found superior (0.59+/-0.01 microg QEs/mg extract).

  17. Analysis of Total Food Intake and Composition of Individual's ...

    EPA Pesticide Factsheets

    EPA released the final report, Analysis of Total Food Intake and Composition of Individual’s Diet Based on USDA’s 1994-1996, 98 Continuing Survey of Food Intakes by Individuals (CSFII). The consumption of food by the general population is a significant route of potential exposure to hazardous substances that are present in the environment. For this reason, a thorough analysis of the dietary habits of the American public would aid in the identification of potential exposure pathways. To that end, the EPA developed per capita food intake rates for various food items and food categories using databases developed by the United States Department of Agriculture (USDA). These intake rates were incorporated into EPA's 1997 Exposure Factors Handbook. Since that time, EPA has recommended that the food intake study be updated and expanded to include a more comprehensive analysis of food intake. That analysis is presented in this document. The purpose of this study is to characterize the consumption of food by the people of the United States.

  18. Species diversity of edaphic mites (Acari: Oribatida) and effects of topography, soil properties and litter gradients on their qualitative and quantitative composition in 64 km² of forest in Amazonia.

    PubMed

    de Moraes, Jamile; Franklin, Elizabeth; de Morais, José Wellington; de Souza, Jorge Luiz Pereira

    2011-09-01

    Small-scale spatial distribution of oribatid mites has been investigated in Amazonia. In addition, medium- and large-scale studies are needed to establish the utility of these mites in detecting natural environmental variability, and to distinguish this variability from anthropogenic impacts. We are expanding the knowledge about oribatid mites in a wet upland forest reserve, and investigate whether a standardized and integrated protocol is an efficient way to assess the effects of environmental variables on their qualitative and quantitative composition on a large spatial scale inside an ecological reserve in Central Amazonia, Brazil. Samples for Berlese-Tullgren extraction were taken in 72 plots of 250 × 6 m distributed over 64 km(2). In total 3,182 adult individuals, from 82 species and 79 morphospecies were recorded, expanding the number of species known in the reserve from 149 to 254. Galumna, Rostrozetes and Scheloribates were the most speciose genera, and 57 species were rare. Rostrozetes ovulum, Pergalumna passimpuctata and Archegozetes longisetosus were the most abundant species, and the first two were the most frequent. Species number and abundance were not correlated with clay content, slope, pH and litter quantity. However, Principal Coordinate Analysis indicated that as the percentage of clay content, litter quantity and pH changed, the oribatid mite qualitative and quantitative composition also changed. The standardized protocol effectively captured the diversity, as we collected one of the largest registers of oribatid mites' species for Amazonia. Moreover, biological and ecological data were integrated to capture the effects of environmental variables accounting for their diversity and abundance.

  19. Changes in ground beetle diversity and community composition in age structured forests (Coleoptera, Carabidae).

    PubMed

    Riley, Kathryn N; Browne, Robert A

    2011-01-01

    We examined diversity, community composition, and wing-state of Carabidae as a function of forest age in Piedmont North Carolina. Carabidae were collected monthly from 396 pitfall traps (12×33 sites) from March 2009 through February 2010, representing 5 forest age classes approximately 0, 10, 50, 85, and 150 years old. A total of 2,568 individuals, representing 30 genera and 63 species, were collected. Carabid species diversity, as estimated by six diversity indices, was significantly different between the oldest and youngest forest age classes for four of the six indices. Most carabid species were habitat generalists, occurring in all or most of the forest age classes. Carabid species composition varied across forest age classes. Seventeen carabid species were identified as potential candidates for ecological indicators of forest age. Non-metric multidimensional scaling (NMDS) showed separation among forest age classes in terms of carabid beetle community composition. The proportion of individuals capable of flight decreased significantly with forest age.

  20. Changes in ground beetle diversity and community composition in age structured forests (Coleoptera, Carabidae)

    PubMed Central

    Riley, Kathryn N.; Browne, Robert A.

    2011-01-01

    Abstract We examined diversity, community composition, and wing-state of Carabidae as a function of forest age in Piedmont North Carolina. Carabidae were collected monthly from 396 pitfall traps (12×33 sites) from March 2009 through February 2010, representing 5 forest age classes approximately 0, 10, 50, 85, and 150 years old. A total of 2,568 individuals, representing 30 genera and 63 species, were collected. Carabid species diversity, as estimated by six diversity indices, was significantly different between the oldest and youngest forest age classes for four of the six indices. Most carabid species were habitat generalists, occurring in all or most of the forest age classes. Carabid species composition varied across forest age classes. Seventeen carabid species were identified as potential candidates for ecological indicators of forest age. Non-metric multidimensional scaling (NMDS) showed separation among forest age classes in terms of carabid beetle community composition. The proportion of individuals capable of flight decreased significantly with forest age. PMID:22371677

  1. Correlates of elemental-isotopic composition of stream fishes: the importance of land-use, species identity and body size.

    PubMed

    Montaña, C G; Schalk, C M

    2018-04-01

    The isotopic (δ 13 C and δ 15 N) and stoichiometric (C:N:P) compositions of four fish species (Family Centrarchidae: Lepomis auritus, Lepomis cyanellus; Family Cyprinidae: Nocomis leptocephalus, Semotilus atromaculatus) were examined across four North Carolina Piedmont streams arrayed along an urbanization gradient. Both isotopic and stoichiometric composition of fishes appeared to track changes occurring in basal resource availability. Values of δ 13 C of basal resources and consumers were more enriched at the most urbanized streams. Similarly, basal resources and consumers were δ 15 N-enriched at more urbanized streams. Basal resource stoichiometry varied across streams, with periphyton being the most variable. Primary consumers stoichiometry also differed across streams. Intraspecific variation in fish stoichiometry correlated with the degree of urbanization, as the two cyprinids had higher N content and L. cyanellus had higher P content in more urbanized streams, probably due to enrichment of basal resources. Intrinsic factors, specifically species identity and body size also affected stoichiometric variation. Phosphorus (P) content increased significantly with body size in centrarchids, but not in cyprinids. These results suggest that although species identity and body size are important predictors of elemental stoichiometry, the complex nature of altered urban streams may yield imbalances in the elemental composition of consumers via their food resources. © 2018 The Fisheries Society of the British Isles.

  2. [Vegetation diversity, composition and structure in a cattle agro-landscape of Matiguás, Nicaragua].

    PubMed

    Merlos, Dalia Sánchez; Harvey, Celia A; Grijalva, Alfredo; Medina, Arnulfo; Vílchez, Sergio; Hernández, Blas

    2005-01-01

    The diversity, composition and structure of vegetation in a cattle landscape in Matiguás, Nicaragua was characterized, and the floristic and structural differences of six types of habitats (secondary forests, riparian forests, charrales, live fences and pastures with high and low tree cover) were compared. A total of 3 949 trees of 180 species and 52 families were recorded. Forty six percent of the total trees reported for the landscape were represented by Guazuma ulmifolia (18.5%), Bursera simaruha (13.2%), Tabebuia rosea (6.3%), Enterolobium cyclocarpum (4.2%) and Albizia saman (3.4%). Many of the dominant species in the landscape were typical of open and disturbed areas. There were significant differences between the different habitats in the patterns of tree species richness, abundance, diversity, structure and floristic composition. The riparian forests had greater tree richness (p=0.0001) and diversity (p=0.0009) than other habitats. The floristic composition varied across habitats. with pairs of habitats sharing between 18.4 and 51.6% of the same tree species, and with clear differences in composition between the forested (riparian and secondary forests) and agricultural habitats. Of the habitats studied, the riparian forests and secondary forests seem to have greatest value for the conservation of the flora in the agropaisaje because they have the greatest species richness, and maintain small populations of endangered species. On the basis of the study, we recommend including agricultural landscapes in strategies to conserve tree diversity and suggest measures to ensure the maintenance of tree diversity in the Matiguas landscape.

  3. Multiscale change in reef coral species diversity and composition in the Tropical Eastern Pacific

    NASA Astrophysics Data System (ADS)

    Gomez, Catalina G.; Gonzalez, Andrew; Guzman, Hector M.

    2018-03-01

    Both natural and anthropogenic factors are changing coral-reef structure and function worldwide. Long-term monitoring has revealed declines in the local composition and species diversity of reefs. Here we report changes in coral-reef community structure over 12 yr (2000-2012) at 17 sites and three spatial scales (reef, gulf and country) in the Tropical Eastern Pacific (Panama). We found a significant 4% annual decline in species population sizes at the country and gulf scales, with significant declines ranging from 3 to 32% at all but one reef. No significant temporal change in expected richness was found at the country scale or in the Gulf of Chiriquí, but a 7% annual decline in expected species richness was found in the Gulf of Panama. There was a 2% increase in community evenness in the Gulf of Chiriquí, but no change in the Gulf of Panama. Significant temporal turnover was found at the country and gulf scales and at 29% of the reefs, a finding mostly explained by changes in species abundance, and losses and gains of rare species. Temporal trends in alpha and beta diversity metrics were explained by water temperature maxima, anomalies and variation that occurred even in the absence of a strong El Niño warming event.

  4. Pericarpial nectary-visiting ants do not provide fruit protection against pre-dispersal seed predators regardless of ant species composition and resource availability

    PubMed Central

    Ré Jorge, Leonardo; Benitez-Vieyra, Santiago; Amorim, Felipe W.

    2017-01-01

    Extrafloral nectaries can occur in both vegetative and reproductive plant structures. In many Rubiaceae species in the Brazilian Cerrado, after corolla abscission, the floral nectary continues to secret nectar throughout fruit development originating post-floral pericarpial nectaries which commonly attract many ant species. The occurrence of such nectar secreting structures might be strategic for fruit protection against seed predators, as plants are expected to invest higher on more valuable and vulnerable parts. Here, we performed ant exclusion experiments to investigate whether the interaction with ants mediated by the pericarpial nectaries of Tocoyena formosa affects plant reproductive success by reducing the number of pre-dispersal seed predators. We also assessed whether ant protection was dependent on ant species composition and resource availability. Although most of the plants were visited by large and aggressive ant species, such as Ectatomma tuberculatum and species of the genus Camponotus, ants did not protect fruits against seed predators. Furthermore, the result of the interaction was neither related to ant species composition nor to the availability of resources. We suggest that these results may be related to the nature and behavior of the most important seed predators, like Hemicolpus abdominalis weevil which the exoskeleton toughness prevent it from being predated by most ant species. On the other hand, not explored factors, such as reward quality, local ant abundance, ant colony characteristics and/or the presence of alternative energetic sources could also account for variations in ant frequency, composition, and finally ant protective effects, highlighting the conditionality of facultative plant-ant mutualisms. PMID:29211790

  5. Weed species composition and distribution pattern in the maize crop under the influence of edaphic factors and farming practices: A case study from Mardan, Pakistan.

    PubMed

    Ahmad, Zeeshan; Khan, Shujaul Mulk; Abd Allah, Elsayed Fathi; Alqarawi, Abdulaziz Abdullah; Hashem, Abeer

    2016-11-01

    Weeds are unwanted plant species growing in ordinary environment. In nature there are a total of 8000 weed species out of which 250 are important for agriculture world. The present study was carried out on weed species composition and distribution pattern with special reference to edaphic factor and farming practices in maize crop of District Mardan during the months of August and September, 2014. Quadrates methods were used to assess weed species distribution in relation to edaphic factor and farming practices. Phytosociological attributes such as frequency, relative frequency, density, relative density and Importance Values were measured by placing 9 quadrates (1 × 1 m 2 ) randomly in each field. Initial results showed that the study area has 29 diverse weed species belonging to 27 genera and 15 families distributed in 585 quadrats. Presence and absence data sheet of 29 weed species and 65 fields were analyzed through PC-ORD version 5. Cluster and Two Way Cluster Analyses initiated four different weed communities with significant indicator species and with respect to underlying environmental variables using data attribute plots. Canonical Correspondence Analyses (CCA) of CANOCO software version 4.5 was used to assess the environmental gradients of weed species. It is concluded that among all the edaphic factors the strongest variables were higher concentration of potassium, organic matter and sandy nature of soil. CCA plots of both weed species and sampled fields based on questionnaire data concluded the farming practices such as application of fertilizers, irrigation and chemical spray were the main factors in determination of weed communities.

  6. Chaetognatha in the Bahía Magdalena lagoon complex, Baja California Sur, México: species composition and assemblages.

    PubMed

    Cota Meza, M S

    2011-07-01

    The chaetognaths from 187 zooplankton samples collected from the Bahia Magdalena lagoon complex, Baja California Sur, Mexico during March, June, July, August, September, November, and December 1982 were studied. Twelve species belonging to two genera were identified. Sagitta euneritica and S. enflata were the most abundant and most frequent species with maximum abundance in July (40,000 org/100 m(3) and 6100 org/100 m(3) respectively). Sagitta pacifica, S. regularis and S. pseudoserratodentata were stenothermic (21 to 25 degrees C), whereas the rest of the species were eurythermic (15.5 to 29.5 degrees C). Sagitta euneritica contributed considerably to the zooplanktonic biomass, increasing the density in particular in BahíaAlmejas. The analysis of the species assemblages (Morisita index) showed that S. pacifica and S. regularis interact more frequently in August when there is a change of the water masses that converge in this zone during summer, when the California Countercurrent predominates. The composition of taxa during winter is characterized by the dominance of S. euneritica. Entering the warm period, an abrupt change occurs in taxa composition of the three zones studied: channels, Bahía Magdalena, and BahíaAlmejas. The amplitude and distribution of S. peruviana was influenced possibility by the oceanographic conditions of ENSO 1982.

  7. Earthworms are associated with subpopulations of Gammaproteobacteria irrespective of the total soil microbiota composition and stability.

    PubMed

    Fjøsne, Trine; Myromslien, Frøydis D; Wilson, Robert C; Rudi, Knut

    2018-05-01

    Soil represents one of the most complex microbial ecosystems on earth. It is well-known that invertebrates such as earthworms have a major impact on transformations of organic material in soil, while their effect on the soil microbiota remains largely unknown. The aim of our work was therefore to investigate the association of earthworms with temporal stability, composition and diversity in two soil microbiota experimental series. We found that earthworms were consistently associated with an increase in subgroups of Gammaproteobacteria, despite major differences in microbiota composition and temporal stability across the experimental series. Our results therefore suggest that earthworms can affect subpopulation dynamics in the soil microbiota, irrespective of the total microbiota composition. If the soil microbiota is comprised of independent microbiota components, this can contribute to our general understanding of the complexity of the soil microbiota.

  8. Characterization of culturable yeast species associating with whole crop corn and total mixed ration silage

    PubMed Central

    Wang, Huili; Hao, Wei; Ning, Tingting; Zheng, Mingli; Xu, Chuncheng

    2018-01-01

    Objective This study investigated the association of yeast species with improved aerobic stability of total mixed ration (TMR) silages with prolonged ensiling, and clarified the characteristics of yeast species and their role during aerobic deterioration. Methods Whole crop corn (WCC) silages and TMR silages formulated with WCC were ensiled for 7, 14, 28, and 56 d and used for an aerobic stability test. Predominant yeast species were isolated from different periods and identified by sequencing analyses of the 26S rRNA gene D1/D2 domain. Characteristics (assimilation and tolerance) of the yeast species and their role during aerobic deterioration were investigated. Results In addition to species of Candida glabrata and Pichia kudriavzevii (P. kudriavzevii) previously isolated in WCC and TMR, Pichia manshurica (P. manshurica), Candida ethanolica (C. ethanolica), and Zygosaccharomyces bailii (Z. bailii) isolated at great frequency during deterioration, were capable of assimilating lactic or acetic acid and tolerant to acetic acid and might function more in deteriorating TMR silages at early fermentation (7 d and 14 d). With ensiling prolonged to 28 d, silages became more (p<0.01) stable when exposed to air, coinciding with the inhibition of yeast to below the detection limit. Species of P. manshurica that were predominant in deteriorating WCC silages were not detectable in TMR silages. In addition, the predominant yeast species of Z. bailii in deteriorating TMR silages at later fermentation (28 d and 56 d) were not observed in both WCC and WCC silages. Conclusion The inhibition of yeasts, particularly P. kudriavzevii, probably account for the improved aerobic stability of TMR silages at later fermentation. Fewer species seemed to be involved in aerobic deterioration of silages at later fermentation and Z. bailii was most likely to initiate the aerobic deterioration of TMR silages at later fermentation. The use of WCC in TMR might not influence the predominant yeast

  9. Characterization of culturable yeast species associating with whole crop corn and total mixed ration silage.

    PubMed

    Wang, Huili; Hao, Wei; Ning, Tingting; Zheng, Mingli; Xu, Chuncheng

    2018-02-01

    This study investigated the association of yeast species with improved aerobic stability of total mixed ration (TMR) silages with prolonged ensiling, and clarified the characteristics of yeast species and their role during aerobic deterioration. Whole crop corn (WCC) silages and TMR silages formulated with WCC were ensiled for 7, 14, 28, and 56 d and used for an aerobic stability test. Predominant yeast species were isolated from different periods and identified by sequencing analyses of the 26S rRNA gene D1/D2 domain. Characteristics (assimilation and tolerance) of the yeast species and their role during aerobic deterioration were investigated. In addition to species of Candida glabrata and Pichia kudriavzevii ( P. kudriavzevii ) previously isolated in WCC and TMR, Pichia manshurica ( P. manshurica ), Candida ethanolica ( C. ethanolica ), and Zygosaccharomyces bailii ( Z. bailii ) isolated at great frequency during deterioration, were capable of assimilating lactic or acetic acid and tolerant to acetic acid and might function more in deteriorating TMR silages at early fermentation (7 d and 14 d). With ensiling prolonged to 28 d, silages became more (p<0.01) stable when exposed to air, coinciding with the inhibition of yeast to below the detection limit. Species of P. manshurica that were predominant in deteriorating WCC silages were not detectable in TMR silages. In addition, the predominant yeast species of Z. bailii in deteriorating TMR silages at later fermentation (28 d and 56 d) were not observed in both WCC and WCC silages. The inhibition of yeasts, particularly P. kudriavzevii , probably account for the improved aerobic stability of TMR silages at later fermentation. Fewer species seemed to be involved in aerobic deterioration of silages at later fermentation and Z. bailii was most likely to initiate the aerobic deterioration of TMR silages at later fermentation. The use of WCC in TMR might not influence the predominant yeast species during aerobic

  10. Grana Padano cheese whey starters: microbial composition and strain distribution.

    PubMed

    Rossetti, Lia; Fornasari, Maria Emanuela; Gatti, Monica; Lazzi, Camilla; Neviani, Erasmo; Giraffa, Giorgio

    2008-09-30

    The aim of this work was to evaluate the species composition and the genotypic strain heterogeneity of dominant lactic acid bacteria (LAB) isolated from whey starter cultures used to manufacture Grana Padano cheese. Twenty-four Grana Padano cheese whey starters collected from dairies located over a wide geographic production area in the north of Italy were analyzed. Total thermophilic LAB streptococci and lactobacilli were quantified by agar plate counting. Population structure of the dominant and metabolically active LAB species present in the starters was profiled by reverse transcriptase, length heterogeneity-PCR (RT-LH-PCR), a culture-independent technique successfully applied to study whey starter ecosystems. The dominant bacterial species were Lactobacillus helveticus, Lactobacillus delbrueckii subsp. lactis, Streptococcus thermophilus, and Lactobacillus fermentum. Diversity in the species composition allowed the whey cultures to be grouped into four main typologies, the one containing L. helveticus, L. delbrueckii subsp. lactis, and S. thermophilus being the most frequent one (45% of the cultures analyzed), followed by that containing only the two lactobacilli (40%). Only a minor fraction of the cultures contained L. helveticus alone (4%) or all the four LAB species (11%). Five hundred and twelve strains were isolated from the 24 cultures and identified by M13-PCR fingerprinting coupled with 16S rRNA gene sequencing. Most of the strains were L. helveticus (190 strains; 37% of the total), L delbrueckii subsp. lactis (90 strains; 18%) and S. thermophilus (215 strains; 42%). This result was in good agreement with the qualitative whey starter composition observed by RT-LH-PCR. M13-PCR fingerprinting indicated a markedly low infra-species diversity, i.e. the same biotypes were often found in more than one culture. The distribution of the biotypes into the different cultures was mainly dairy plant-specific rather than correlated with the different production areas.

  11. Landscape effects on structure and species composition of tabonuco forests in Puerto Rico: implications for conservation

    Treesearch

    Migdalia Alvarez Ruiz; Ariel E. Lugo

    2012-01-01

    We studied the structure and species composition of nine residual forest stands of Dacryodes excelsa (tabonuco), a dominant vegetation type in the moist and wet lower montane forests of the Caribbean. The stands were scattered over three different landscapes with different degrees of anthropogenic disturbance: forested, shade coffee, and tobacco. We compared our...

  12. Earthworm abundance and species composition in abandoned tropical croplands: comparisons of tree plantations and secondary forests.

    Treesearch

    G. Gonzalez; X. Zou; S. Borges

    1996-01-01

    We compared patterns of earthworms abundance and species composition in tree plantation and secondary forest of Puerto Rico. Tree plantations included pine (Pinus caribea Morelet) and mahogany (Swietenia macrophylla King) established in the 1930's; 1960's; and 1970's; secondary forests were naturally regenerated in areas adjacent to these plantations. We...

  13. Ciliate protozoa in the forestomach of the dromedary camel, (Camelus dromedarius), in Egypt, with description of a new species.

    PubMed

    Selim, H M; Imai, S; Yamato, O; Miyagawa, E; Maede, Y

    1996-09-01

    The composition of ciliates obtained from the forestomachs of eleven dromedary (one-humped) camels in Egypt was examined. As a result, eight genera containing 24 species with 11 forms were identified. Of them, one species was concluded to be new, then described as Dasytricha kabanii n. sp. This new species was clearly distinguished from D. ruminantium, the other species of the genus, by its lack of somatic cilia on the posterior one-fifth of the body surface. Entodinium nanellum and Epidinium ecaudatum f. caudatum were found in all camels examined. Although the percentage composition of respective species varied with the individual camel, the rate of Entodinium spp. was high in general. Total ciliate density in forestomach fluid was 1.9 x 10(5)/ml on average. Ciliate composition in Egyptian camels was similar to that in Bactrian camels, Camelus bactrianus, in China reported previously. However, more Entodinium species were detected from Egyptian camels than from Bactrian camels.

  14. Antimicrobial Activity and Chemical Composition of Essential Oils from Verbenaceae Species Growing in South America.

    PubMed

    Pérez Zamora, Cristina M; Torres, Carola A; Nuñez, María B

    2018-03-01

    The Verbenaceae family includes 2600 species grouped into 100 genera with a pantropical distribution. Many of them are important elements of the floras of warm-temperature and tropical regions of America. This family is known in folk medicine, and its species are used as digestive, carminative, antipyretic, antitussive, antiseptic, and healing agents. This review aims to collect information about the essential oils from the most reported species of the Verbenaceae family growing in South America, focusing on their chemical composition, antimicrobial activity, and synergism with commercial antimicrobials. The information gathered comprises the last twenty years of research within the South American region and is summarized taking into consideration the most representative species in terms of their essential oils. These species belong to Aloysia , Lantana , Lippia , Phyla , and Stachytarpheta genera, and the main essential oils they contain are monoterpenes and sesquiterpenes, such as β-caryophyllene, thymol, citral, 1,8-cineole, carvone, and limonene. These compounds have been found to possess antimicrobial activities. The synergism of these essential oils with antibiotics is being studied by several research groups. It constitutes a resource of interest for the potential use of combinations of essential oils and antibiotics in infection treatments.

  15. Effect of growth phase on the fatty acid compositions of four species of marine diatoms

    NASA Astrophysics Data System (ADS)

    Liang, Ying; Mai, Kangsen

    2005-04-01

    The fatty acid compositions of four species of marine diatoms ( Chaetoceros gracilis MACC/B13, Cylindrotheca fusiformis MACC/B211, Phaeodactylum tricornutum MACC/B221 and Nitzschia closterium MACC/B222), cultivated at 22°C±1°C with the salinity of 28 in f/2 medium and harvested in the exponential growth phase, the early stationary phase and the late stationary phase, were determined. The results showed that growth phase has significant effect on most fatty acid contents in the four species of marine diatoms. The proportions of 16:0 and 16:1n-7 fatty acids increased while those of 16:3n-4 and eicosapentaenoic acid (EPA) decreased with increasing culture age in all species studied. The subtotal of saturated fatty acids (SFA) increased with the increasing culture age in all species with the exception of B13. The subtotal of monounsaturated fatty acids (MUFA) increased while that of polyunsaturated fatty acids (PUFA) decreased with culture age in the four species of marine diatoms. MUFA reached their lowest value in the exponential growth phase, whereas PUFA reached their highest value in the same phase.

  16. Species diversity and composition in old growth and second growth rich coves of the southern Appalachian Mountains

    Treesearch

    Clay Jackson; Dan Pitillo; Lee Allen; Thomas R Wnetworth; Bronson P Bullock; David L. Loftis

    2009-01-01

    Because of ongoing debate over the long term impacts of logging, we conducteda study to assess if second growth (70 6 10 years) rich coves differ from old growth rich coves(. 125 years) in species diversity or composition. We sampled twenty-six 0.1 ha...

  17. Fabrication and wear test of a continuous fiber/particulate composite total surface hip replacement

    NASA Technical Reports Server (NTRS)

    Roberts, J. C.; Ling, F. F.; Jones, W. R., Jr.

    1981-01-01

    Continuous fiber woven E-glass composite femoral shells having the ame elastic properties as bone were fabricated. The shells were then encrusted with filled epoxy wear resistant coatings and run dry against ultrahigh molecular weight polyethylene acetabular cups in 42,000 and 250,000 cycle were tests on a total hip simulator. The tribological characteristics of these shells atriculating with the acetabular cups are comparable to a vitallium bal articulating with an ultrahigh molecular weight polyethylene cup.

  18. Bark anatomy, chemical composition and ethanol-water extract composition of Anadenanthera peregrina and Anadenanthera colubrina

    PubMed Central

    Sartori, Caroline J.; Miranda, Isabel; Quilhó, Teresa; Mori, Fábio Akira; Pereira, Helena

    2017-01-01

    The bark of Anadenanthera peregrina (L.) Speg and Anadenanthera colubrina (Vell.) Brenan were characterized in relation to anatomical and chemical features. The barks were similar and included a thin conducting phloem, a largely dilated and sclerified non-conducting phloem, and a rhyridome with periderms with thin phellem interspersed by cortical tissues. Only small differences between species were observed that cannot be used alone for taxonomic purposes. The summative chemical composition of A. peregrina and A. colubrina was respectively: 8.2% and 7.7% ash; 28.8% and 29.3% extractives; 2.4% and 2.6% suberin; and 18.9% lignin. The monosaccharide composition showed the predominance of glucose (on average 82% of total neutral sugars) and of xylose (9%). The ethanol-water extracts of A. peregrina and A. colubrina barks included a high content of phenolics, respectively: total phenolics 583 and 682 mg GAE/g extract; 148 and 445 mg CE/g extract; tannins 587 and 98 mg CE/g extract. The antioxidant activity was 238 and 269 mg Trolox/g extract. The barks of the Anadenanthera species are a potential source of polar extractives that will represent an important valorization and therefore contribute to improve the overall economic potential and sustainability of A. peregrina and A. colubrina PMID:29281656

  19. Seaweed composition from Bintulu coast of Sarawak, Malaysia.

    PubMed

    Zawawi, Mohd Hafizbillah; Idris, Mohd Hanafi; Kamal, Abu Hena Mustafa; King, Wong Sing

    2014-08-01

    Species composition of seaweed and distribution were investigated in the coastal waters of Bintulu, Sarawak. The seaweed samples were collected during low tide between May 2011 and May 2012 from the six different stations. In total 54 species of seaweeds were identified from study areas of Bintulu coastal waters. Among them, 23 species were from Rhodophyta with 11 families, 15 species were from Phaeophyta with 2 families and 16 species were from Chlorophyta with 10 families: Seventeen species of seaweeds were recorded from the Tanjung Batu, while 23 species from Pantai Telekom, 14 species from Golden Beach, 26 species from Kuala Similajau, 12 species from Kuala Nyalau and 21 species from Batu Mandi. Seaweeds abundance was high in rocky substrate and Rhodophyta (11 families and 23 species) was the common and highest group of seaweeds in this coastal areas. Present study recorded high diversified seaweed species at the rocky shore area compare to reef area.

  20. Comparison of proximate composition and sensory attributes of Clariid catfish species of Clarias gariepinus, Heterobranchus bidorsalis, and their hybrids.

    PubMed

    Olaniyi, Wasiu A; Makinde, Olukayode A; Omitogun, Ofelia G

    2017-03-01

    Clariid catfish are favorite food fish especially in African and Asian continents. Recently there has been preference for particular species or hybrids of these species based on quality assurance and value addition. Consequently, this study aimed to evaluate the possible effect of different catfish species and their hybrids on proximate composition and sensory attributes. Catfish species, Clarias gariepinus (CC), Heterobranchus bidorsalis (HH), with their hybrid (CH), and reciprocal hybrid (HC) were evaluated for sensory variables - cognitive (sweet, salty, sour, bitter, and recent characteristic taste 'umami' ) and qualitative (texture, aroma, flavor, and color) tests; and nutritional variables - proximate composition (moisture, protein, ether/fat, and ash). A 5-point hedonic scale from 'neutral/neither like nor dislike' to 'excellent/like extremely' was employed in sensory testing. The results showed similar ( P  > 0.05) high moisture contents (>70%) in all species and high but different ( P  < 0.05) ash contents (11-14%) that suggested good sources of mineral elements. The parent species CC and HH had higher ash contents than CH or HC. The crude protein contents were high and similar ( P  > 0.05) across species (>57%). Fat or ether extract was different ( P  < 0.05) and tended to be higher for species with Clarias as the female parent than Heterobranchus . Sensory analysis showed the parent species, CC and HH, more favorably rated for sweet and umami than the hybrids, CH and HC. However, CH was less sour and bitter than all other species and HC better than CH for salty but similar to CC and HH. All fish species were very well liked for texture, but the parent species were superior in flavor than the hybrids. All species were very well liked for aroma, color, and overall acceptability except HC, which was moderately liked. HC rated inferior to the other species overall in sensory attributes. All the fish species did not rate 'excellent/like extremely' for

  1. Impact of soil scarification on the composition of regeneration and species diversity in an oak shelterwood

    Treesearch

    James J. Zaczek; Joseph Harding; James Welfley

    1997-01-01

    This study was conducted in a fenced 1-yr-old 70-acre mixed-oak shelterwood to determine the impact of soil scarification on species composition and the production of oak regeneration from abundant northern red oak (Quercus rubra L.) acorns. In October 1993, seven replicates were established and randomly divided into control and scarified plots....

  2. Coral Symbiodinium Community Composition Across the Belize Mesoamerican Barrier Reef System is Influenced by Host Species and Thermal Variability.

    PubMed

    Baumann, J H; Davies, S W; Aichelman, H E; Castillo, K D

    2018-05-01

    Reef-building corals maintain a symbiotic relationship with dinoflagellate algae of the genus Symbiodinium, and this symbiosis is vital for the survival of the coral holobiont. Symbiodinium community composition within the coral host has been shown to influence a coral's ability to resist and recover from stress. A multitude of stressors including ocean warming, ocean acidification, and eutrophication have been linked to global scale decline in coral health and cover in recent decades. Three distinct thermal regimes (high TP , mod TP , and low TP ) following an inshore-offshore gradient of declining average temperatures and thermal variation were identified on the Belize Mesoamerican Barrier Reef System (MBRS). Quantitative metabarcoding of the ITS-2 locus was employed to investigate differences and similarities in Symbiodinium genetic diversity of the Caribbean corals Siderastrea siderea, S. radians, and Pseudodiploria strigosa between the three thermal regimes. A total of ten Symbiodinium lineages were identified across the three coral host species. S. siderea was associated with distinct Symbiodinium communities; however, Symbiodinium communities of its congener, S. radians and P. strigosa, were more similar to one another. Thermal regime played a role in defining Symbiodinium communities in S. siderea but not S. radians or P. strigosa. Against expectations, Symbiodinium trenchii, a symbiont known to confer thermal tolerance, was dominant only in S. siderea at one sampled offshore site and was rare inshore, suggesting that coral thermal tolerance in more thermally variable inshore habitats is achieved through alternative mechanisms. Overall, thermal parameters alone were likely not the only primary drivers of Symbiodinium community composition, suggesting that environmental variables unrelated to temperature (i.e., light availability or nutrients) may play key roles in structuring coral-algal communities in Belize and that the relative importance of these

  3. Exploring the Gastrointestinal "Nemabiome": Deep Amplicon Sequencing to Quantify the Species Composition of Parasitic Nematode Communities.

    PubMed

    Avramenko, Russell W; Redman, Elizabeth M; Lewis, Roy; Yazwinski, Thomas A; Wasmuth, James D; Gilleard, John S

    2015-01-01

    Parasitic helminth infections have a considerable impact on global human health as well as animal welfare and production. Although co-infection with multiple parasite species within a host is common, there is a dearth of tools with which to study the composition of these complex parasite communities. Helminth species vary in their pathogenicity, epidemiology and drug sensitivity and the interactions that occur between co-infecting species and their hosts are poorly understood. We describe the first application of deep amplicon sequencing to study parasitic nematode communities as well as introduce the concept of the gastro-intestinal "nemabiome". The approach is analogous to 16S rDNA deep sequencing used to explore microbial communities, but utilizes the nematode ITS-2 rDNA locus instead. Gastro-intestinal parasites of cattle were used to develop the concept, as this host has many well-defined gastro-intestinal nematode species that commonly occur as complex co-infections. Further, the availability of pure mono-parasite populations from experimentally infected cattle allowed us to prepare mock parasite communities to determine, and correct for, species representation biases in the sequence data. We demonstrate that, once these biases have been corrected, accurate relative quantitation of gastro-intestinal parasitic nematode communities in cattle fecal samples can be achieved. We have validated the accuracy of the method applied to field-samples by comparing the results of detailed morphological examination of L3 larvae populations with those of the sequencing assay. The results illustrate the insights that can be gained into the species composition of parasite communities, using grazing cattle in the mid-west USA as an example. However, both the technical approach and the concept of the 'nemabiome' have a wide range of potential applications in human and veterinary medicine. These include investigations of host-parasite and parasite-parasite interactions during co

  4. Onchocerciasis in the Amazonian focus of southern Venezuela: altitude and blackfly species composition as predictors of endemicity to select communities for ivermectin control programmes.

    PubMed

    Vivas-Martinez, S; Basáñez, M G; Grillet, M E; Weiss, H; Botto, C; García, M; Villamizar, N J; Chavasse, D C

    1998-01-01

    In preparation for an ivermectin distribution programme, the prevalence and intensity of infection due to Onchocerca volvulus as well as the species composition and abundance of Simulium vectors were investigated in 22 Yanomami communities situated along 2 altitudinal transects in the southern Venezuelan onchocerciasis focus. These transects corresponded to the Ocamo-Putaco and Orinoco-Orinoquito river systems, covering a range of elevation between 50 m and 740 m above sea level (asl). A total of 831 people underwent parasitological examination in this survey and an additional 196 patients from a previous study, at an altitude of 950 m, were included in the analysis. A total of 92,659 man-biting blackflies were collected and identified to morphospecies. S. oyapockense s.l. was the predominant simuliid up to 150 m asl, whereas S. guianense s.l. and S. incrustatum s.l. prevailed above 150 m. Communities located below 150 m were found to range from hypo- to mesoendemic; all villages above 150 m proved to be hyperendemic (> 60% microfilarial prevalence) and mass ivermectin treatment should be implemented. Age above 10-14 years, altitude of the village and biting rate of S. guianense s.l. up to 200 m asl were found to be statistically significant independent predictors of infection by multivariate logistic regression using a spline model. There were no differences in infection status according to sex. Above 200 m, microfilarial rate and density remained approximately constant, prevalence averaging 79% regardless of blackfly abundance. For the implementation of ivermectin-based onchocerciasis control programmes in the Amazonian focus, altitude and species composition of the blackfly population might be adopted as useful indicators aiding selection of the most affected communities. However, below 200 m additional parasitological indicators may also be necessary. As a direct result of this study, regular mass-ivermectin delivery to meso- and hyperendemic communities is now

  5. Changes in the biomass and species composition of macroalgae in a eutrophic estuary

    NASA Astrophysics Data System (ADS)

    Lavery, Paul S.; Lukatelich, R. J.; McComb, A. J.

    1991-07-01

    More than 20 years of data are presented on the macroalgal biomass, species composition and water quality of Peel-Harvey estuary in south-western Australia. The occurrence of macroalgal blooms was a sudden event in the late 1960s, and appears to have resulted from nutrient availability surpassing a threshold of some kind. Cladophora dominated the system until 1979 and appears to have had a competitive advantage in deep-water areas because of its morphology. A catastrophic event compounded by a series of unfavourable conditions resulted in the loss of Cladophora from the deep areas and its estuary-wide replacement by Chaetomorpha, which was more competitive in the shallows. Since 1979, changes in water quality have been reflected in changes in biomass and species composition in the system. Average annual biomass is linearly related to average light attenuation over the summer growth period. Periods of high nutrient concentrations favour Ulva and Enteromorpha, while Chaetomorpha resumes dominance during periods of lower mean nutrient concentrations. Nutrient concentrations appear to be more influential on an inter-annual than seasonal scale, except in the case of Ulva which, on the basis of tissue N and P concentrations, is seasonally nitrogen-limited. Light attenuation appears to have seasonal and long-term effects. The data support the hypothesis of other workers that inter-annual differences in hydrographic events and phytoplankton dynamics influence macroalgal dynamics. The concept is examined further in light of this extensive database.

  6. Factors affecting Archaeal Lipid Compositions of the Sulfolobus Species

    NASA Astrophysics Data System (ADS)

    He, L.; Han, J.; Wei, Y.; Lin, L.; Wei, Y.; Zhang, C.

    2010-12-01

    Temperature is the best known variable affecting the distribution of the archaeal glycerol dibiphytanyl glycerol tetraethers (GDGTs) in marine and freshwater systems. Other variables such as pH, ionic strength, or bicarbonate concentration may also affect archaeal GDGTs in terrestrial systems. Studies of pure cultures can help us pinpoint the specific effects these variables may have on archaeal lipid distribution in natural environments. In this study, three Sulfolobus species (HG4, HB5-2, HB9-6) isolated from Tengchong hot springs (pH 2-3, temperature 73-90°C) in China were used to investigate the effects of temperature, pH, substrate, and type of strain on the composition of GDGTs. Results showed that increase in temperature had negative effects on the relative contents of GDGT-0 (no cyclopentyl rings), GDGT-1 (one cyclopentyl ring), GDGT-2 and GDGT-3 but positive effects on GDGT-4, GDGT-4', GDGT-5 and GDGT-5'. Increase in pH, on the other hand, had negative effects on GDGT-0, GDGT-1, GDGT-4', GDGT-5 and GDGT-5', and positive effects on GDGT-3 and GDGT-4. GDGT-2 remained relatively constant with changing pH. When the HG4 was grown on different substrates, GDGT-5 was five time more abundant in sucrose-grown cultures than in yeast extract- or sulfur- grown cultures, suggesting that carbohydrates may stimulate the production of GDGT-5. For all three species, the ring index (average number of rings) of GDGTs correlated positively with incubation temperature. In HG4, ring index was much lower at optimal pH (3.5) than at other pH values. Ring index of HB5-2 or HB9-6 is higher than that of HG4, suggesting that speciation may affect the degree of cyclization of GDGT of the Sulfolobus. These results indicate that individual archaeal lipids respond differently to changes in environmental variables, which may be also species specific.

  7. Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India.

    PubMed

    Subramanya, M D; Pai, Sandeep R; Upadhya, Vinayak; Ankad, Gireesh M; Bhagwat, Shalini S; Hegde, Harsha V

    2015-01-01

    Sida L., is a medicinally important genus, the species of which are widely used in traditional systems of medicine in India. Pharmacologically, roots are known for anti-tumor, anti-HIV, hepatoprotective, and many other properties. Phenolic antioxidants help in reducing oxidative stress occurring during treatment of such diseases. The study aimed to evaluate and compare polyphenol contents and antioxidant properties of eight selected species of Sida from Western Ghats, India. Methanolic root extracts (10% w/v) of Sida species, viz., S. acuta, S. cordata, S. cordifolia, S. indica, S. mysorensis, S. retusa, S. rhombifolia, and S. spinosa were analyzed. Sida cordifolia possessed highest total phenolic content (TPC: 1.92 ± 0.10 mg Caffeic Acid Equivalent/g and 2.13 ± 0.11 mg Tannic Acid Equivalant/g), total flavonoid content (TF: 2.60 ± 0.13 mg Quercetin Equivalent/g) and also possessed highest antioxidant activities in 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging (51.31 ± 2.57% Radical Scavenging Activity, (RSA); Trolox Equivalent Antioxidant Capacity: 566.25 ± 28.31μM; Ascorbic acid Equivalent Antioxidant Capacity: 477.80 ± 23.89 μM) and Ferric Reducing Antioxidant Power assays (TEAC: 590.67 ± 29.53 μM; AEAC: 600.67 ± 30.03 μM). Unlike DPPH and Ferric Reducing Antioxidant Power (FRAP) activity, 2, 2'-Azinobis (3-ethyl Benzo Thiazoline-6-Sulfonic acid) ABTS(+) antioxidant activity was highest in S. indica (TEAC: 878.44 ± 43.92 μM; AEAC 968.44 ± 48.42 μM). It was significant to note that values of AEAC (μM) for all the antioxidant activities analyzed were higher than that of TEAC. The high contents of phenolic compounds in the root extracts of selected Sida species have direct correlation with their antioxidant properties. Conclusively, roots of S. cordifolia can be considered as the potential source of polyphenols and antioxidants.

  8. Proximate composition, total phenolic content, and antioxidant activity of seagrape (Caulerpa lentillifera).

    PubMed

    Nguyen, Van Tang; Ueng, Jinn-Pyng; Tsai, Guo-Jane

    2011-09-01

    The proximate composition of seagrape (Caulerpa lentillifera) from culture ponds in Penghu, Taiwan was analyzed. The phenolic content and the antioxidant activities including the 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activity, ferric ion-reducing activity, hydrogen peroxide scavenging activity, and ferrous ion chelating (FIC) activity of the ethanolic extracts of dry seagrape samples using 2 drying methods of freeze drying and thermal drying were compared with the ethanolic extract of Oolong tea as a reference. The contents (dry weight basis) of carbohydrate, crude protein, crude lipid, crude fiber, and ash of seagrape obtained from culture ponds in Taiwan were 64.00%, 9.26%, 1.57%, 2.97%, and 22.20%, respectively. The total phenolic content (1.30 mg gallic acid equivalent [GAE]/g dry weight) of the ethanolic extract of thermally dried seagrape was significantly lower (P < 0.05) than that (2.04 mg GAE/g dry weight) of freeze-dried seagrape, and both were significantly lower than that (13.58 mg GAE/g dry weight) of Oolong tea. At the same phenolic content, the antioxidant activities of freeze-dried seagrape were significantly higher (P < 0.05) than those of thermally dried seagrape. Compared with Oolong tea, seagrape, irrespective of drying method used, generally had strong hydrogen peroxide scavenging activity; but it was weak in DPPH radical scavenging activity, ferric ion-reducing activity, and FIC activity. The antioxidant activity of seagrape and Oolong tea was significantly influenced by their phenolic contents. The proximate composition, total phenolic content, and antioxidant activity of seagrape (Caulerpa lentillifera) in Taiwan were determined in this research to indicate nutritionally of this edible seaweed to human health, and compared these results to previous studies. © 2011 Institute of Food Technologists®

  9. A Pine Is a Pine and a Spruce Is a Spruce--The Effect of Tree Species and Stand Age on Epiphytic Lichen Communities.

    PubMed

    Bäcklund, Sofia; Jönsson, Mari; Strengbom, Joachim; Frisch, Andreas; Thor, Göran

    2016-01-01

    With an increasing demand for forest-based products, there is a growing interest in introducing fast-growing non-native tree species in forest management. Such introductions often have unknown consequences for native forest biodiversity. In this study, we examine epiphytic lichen species richness and species composition on the trunks of non-native Pinus contorta and compare these to the native Pinus sylvestris and Picea abies in managed boreal forests in northern Sweden across a chronosequence of age classes. Overall, we recorded a total of 66,209 lichen occurrences belonging to 57 species in the 96 studied forest stands. We found no difference in species richness of lichens between stands of P. contorta and P. sylvestris, but stands of P. abies had higher total species richness. However, species richness of lichens in stands of P. abies decreased with increasing stand age, while no such age effect was detected for P. contorta and P. sylvestris. Lichen species composition progressively diverged with increasing stand age, and in 30-year-old stands all three tree species showed species-specific assemblages. Epiphytic lichen assemblages in stands of 30-year-old P. contorta were influenced by greater basal area, canopy closure, and average diameter at breast height, P. abies stands by higher branch density and canopy closure, and stands of P. sylvestris by greater bark crevice depth. Differences in lichen species richness and composition were mainly explained by canopy closure and habitat availability, and the greater canopy closure in mature P. abies stands promoted the colonization and growth of calicioid lichen species. Our results indicate that the non-native P. contorta have similar species richness as the native P. sylvestris. The main difference in lichen species richness and composition is between P. abies and Pinus spp. in managed forests of boreal Sweden.

  10. Patterns of vegetation and grasshopper community composition.

    PubMed

    Kemp, W P; Harvey, S J; O'Neill, K M

    1990-06-01

    A study was conducted to evaluate differences in rangeland grasshopper communities over environmental gradients in Gallatin Valley, Montana, USA. The concept of habitat type (Daubenmire 1966) was used as a basis for discriminating between groupings of patches based on vegetation. A total of 39 patches were selected that represented five recognized grassland habitat types (Mueggler and Stewart 1980), as well as two disturbed types (replanting within a known habitat type). Repeated sampling in 1988 of both the insect and plant communities yielded a total of 40 grasshopper (19 664 individuals) and 97 plant species. Detrended Correspondence Analysis (DCA) indicated that patch classifications based on presence and percent cover of plants were appropriate and showed good between-group (habitat type) separation for patches along gradients of precipitation/elevation and plant community complexity. Results from undisturbed habitats showed that plant and grasshopper species composition changed over observed environmental gradients and suggested that habitat type influenced not only species presence, but also relative abundance. Discussion is presented that relates results with patch-use and core and satellite species paradigms.

  11. Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves.

    PubMed

    Sebei, Khaled; Sakouhi, Fawzi; Herchi, Wahid; Khouja, Mohamed Larbi; Boukhchina, Sadok

    2015-01-19

    In this paper, we have studied the essential oils chemical composition of the leaves of seven Eucalyptus species developed in Tunisia. Eucalyptus leaves were picked from trees growing in different arboretums in Tunisia. Choucha and Mrifeg arboretums located in Sedjnene, region of Bizerte (Choucha: E. maideni, E. astrengens et E. cinerea; Mrifeg : E. leucoxylon), Korbous arboretums located in the region of Nabeul, North East Tunisia with sub-humid bioclimate, (E. lehmani), Souiniet-Ain Drahem arboretum located in region of Jendouba (E. sideroxylon, E. bicostata). Essential oils were individually tested against a large panel of microorganisms including Staphylococcus aureus (ATCC 6539), Escherichia coli (ATCC 25922), Enterococcus faecalis (ATCC29212), Listeria ivanovii (RBL 30), Bacillus cereus (ATCC11778). The yield of essential oils ranged from 1.2% to 3% (w/w) for the different Eucalyptus species. All essential oils contain α-pinene, 1,8-cineol and pinocarveol-trans for all Eucalyptus species studied. The 1,8-cineol was the major compound in all species (49.07 to 83.59%). Diameter of inhibition zone of essential oils of Eucalyptus species varied from 10 to 29 mm. The largest zone of inhibition was obtained for Bacillus cereus (E. astrengens) and the lowest for Staphylococcus aureus (E. cinerea). The essential oils from E. maideni, E. astrengens, E. cinerea (arboretum of Bizerte), E. bicostata (arboretum of Aindraham) showed the highest antibacterial activity against Listeria ivanovii and Bacillus cereus. The major constituents of Eucalyptus leaves essential oils are 1,8-cineol (49.07 to 83.59%) and α-pinene (1.27 to 26.35%). The essential oils from E. maideni, E. astrengens, E. cinerea, E. bicostata showed the highest antibacterial activity against Listeria ivanovii and Bacillus cereus, they may have potential applications in food and pharmaceutical products.

  12. Transylvanian Composite Total Petroleum System of the Transylvanian Basin Province, Romania, Eastern Europe

    USGS Publications Warehouse

    Pawlewicz, Mark

    2005-01-01

    The Transylvanian Composite Total Petroleum System and the Transylvanian Neogene Suprasalt Gas Assessment Unit were identified in the Transylvanian Basin Province (4057), which lies entirely within the country of Romania. The assessment unit is composed of middle Miocene (Badenian) to Pliocene strata. Gas from the assessment unit is biogenic and originated from bacterial activity on dispersed organic matter in shales and siltstones. Gas migration is believed to be minimal, both vertically and laterally, with gases trapped in local stratigraphic traps and in structural traps (most likely domes and anticlinal folds created by salt diapirism) in the central part of the basin. For this reason, the gas fields also are concentrated in the central part of the basin. Reservoirs are isolated and composed of vertically stacked sandstones and siltstones sealed by shales and possibly by evaporite layers. Traps result from stratigraphic and facies changes within the entire assessment unit stratigraphic section. Some gas fields contain as much or more than a trillion cubic feet, but in most, the estimated resources are between 6 billion and 96 billion cubic feet. Petroleum exploration in the province is in the mature stage, which, combined with the geologic complexity of the region, is considered to limit future discoveries to a relatively few small fields. The undiscovered resources for the Transylvanian Basin Neogene Suprasalt Composite AU in the Transylvanian Hybrid Total Petroleum System (4057) are, at the mean, 2.083 trillion cubic feet of gas. No oil is produced in the basin. Rocks underlying the salt layers were not assessed for hydrocarbon potential.

  13. Lipid composition analysis of milk fats from different mammalian species: potential for use as human milk fat substitutes.

    PubMed

    Zou, Xiaoqiang; Huang, Jianhua; Jin, Qingzhe; Guo, Zheng; Liu, Yuanfa; Cheong, Lingzhi; Xu, Xuebing; Wang, Xingguo

    2013-07-24

    The lipid compositions of commercial milks from cow, buffalo, donkey, sheep, and camel were compared with that of human milk fat (HMF) based on total and sn-2 fatty acid, triacylglycerol (TAG), phospholipid, and phospholipid fatty acid compositions and melting and crystallization profiles, and their degrees of similarity were digitized and differentiated by an evaluation model. The results showed that these milk fats had high degrees of similarity to HMF in total fatty acid composition. However, the degrees of similarity in other chemical aspects were low, indicating that these milk fats did not meet the requirements of human milk fat substitutes (HMFSs). However, an economically feasible solution to make these milks useful as raw materials for infant formula production could be to modify these fats, and a possible method is blending of polyunsaturated fatty acids (PUFA) and 1,3-dioleoyl-2-palmitoylglycerol (OPO) enriched fats and minor lipids based on the corresponding chemical compositions of HMF.

  14. Hawaiian sandalwood: oil composition of Santalum paniculatum and comparison with other sandal species.

    PubMed

    Braun, Norbert A; Sim, Sherina; Kohlenberg, Birgit; Lawrence, Brian M

    2014-09-01

    Four commercial qualities of Hawaiian sandalwood oil produced from wood of Santalum paniculatum originating from the island of Hawaii ("The Big Island") were analyzed using GC and GC-MS. Main constituents of the oils were (Z)-α-santalol (34.5-40.4%) and (Z)-β-santalol (11.0-16.2%). An odor evaluation of the oils was carried out against East Indian sandalwood oil. In addition, the chemical composition of Hawaiian sandalwood oil was compared with four different Santalum species originating from East India, New Caledonia, Eastern Polynesia and Australia, respectively.

  15. Climate Extreme Effects on the Chemical Composition of Temperate Grassland Species under Ambient and Elevated CO2: A Comparison of Fructan and Non-Fructan Accumulators

    PubMed Central

    Zinta, Gaurav; Van den Ende, Wim; Janssens, Ivan A.; Asard, Han

    2014-01-01

    Elevated CO2 concentrations and extreme climate events, are two increasing components of the ongoing global climatic change factors, may alter plant chemical composition and thereby their economic and ecological characteristics, e.g. nutritional quality and decomposition rates. To investigate the impact of climate extremes on tissue quality, four temperate grassland species: the fructan accumulating grasses Lolium perenne, Poa pratensis, and the nitrogen (N) fixing legumes Medicago lupulina and Lotus corniculatus were subjected to water deficit at elevated temperature (+3°C), under ambient CO2 (392 ppm) and elevated CO2 (620 ppm). As a general observation, the effects of the climate extreme were larger and more ubiquitous in combination with elevated CO2. The imposed climate extreme increased non-structural carbohydrate and phenolics in all species, whereas it increased lignin in legumes and decreased tannins in grasses. However, there was no significant effect of climate extreme on structural carbohydrates, proteins, lipids and mineral contents and stoichiometric ratios. In combination with elevated CO2, climate extreme elicited larger increases in fructan and sucrose content in the grasses without affecting the total carbohydrate content, while it significantly increased total carbohydrates in legumes. The accumulation of carbohydrates in legumes was accompanied by higher activity of sucrose phosphate synthase, sucrose synthase and ADP-Glc pyrophosphorylase. In the legumes, elevated CO2 in combination with climate extreme reduced protein, phosphorus (P) and magnesium (Mg) contents and the total element:N ratio and it increased phenol, lignin, tannin, carbon (C), nitrogen (N) contents and C:N, C:P and N:P ratios. On the other hand, the tissue composition of the fructan accumulating grasses was not affected at this level, in line with recent views that fructans contribute to cellular homeostasis under stress. It is speculated that quality losses will be less

  16. Climate extreme effects on the chemical composition of temperate grassland species under ambient and elevated CO2: a comparison of fructan and non-fructan accumulators.

    PubMed

    AbdElgawad, Hamada; Peshev, Darin; Zinta, Gaurav; Van den Ende, Wim; Janssens, Ivan A; Asard, Han

    2014-01-01

    Elevated CO2 concentrations and extreme climate events, are two increasing components of the ongoing global climatic change factors, may alter plant chemical composition and thereby their economic and ecological characteristics, e.g. nutritional quality and decomposition rates. To investigate the impact of climate extremes on tissue quality, four temperate grassland species: the fructan accumulating grasses Lolium perenne, Poa pratensis, and the nitrogen (N) fixing legumes Medicago lupulina and Lotus corniculatus were subjected to water deficit at elevated temperature (+3°C), under ambient CO2 (392 ppm) and elevated CO2 (620 ppm). As a general observation, the effects of the climate extreme were larger and more ubiquitous in combination with elevated CO2. The imposed climate extreme increased non-structural carbohydrate and phenolics in all species, whereas it increased lignin in legumes and decreased tannins in grasses. However, there was no significant effect of climate extreme on structural carbohydrates, proteins, lipids and mineral contents and stoichiometric ratios. In combination with elevated CO2, climate extreme elicited larger increases in fructan and sucrose content in the grasses without affecting the total carbohydrate content, while it significantly increased total carbohydrates in legumes. The accumulation of carbohydrates in legumes was accompanied by higher activity of sucrose phosphate synthase, sucrose synthase and ADP-Glc pyrophosphorylase. In the legumes, elevated CO2 in combination with climate extreme reduced protein, phosphorus (P) and magnesium (Mg) contents and the total element:N ratio and it increased phenol, lignin, tannin, carbon (C), nitrogen (N) contents and C:N, C:P and N:P ratios. On the other hand, the tissue composition of the fructan accumulating grasses was not affected at this level, in line with recent views that fructans contribute to cellular homeostasis under stress. It is speculated that quality losses will be less

  17. Dynamics of change in total and regional body composition after gastric bypass in obese patients.

    PubMed

    Ciangura, Cecile; Bouillot, Jean-Luc; Lloret-Linares, Celia; Poitou, Christine; Veyrie, Nicolas; Basdevant, Arnaud; Oppert, Jean-Michel

    2010-04-01

    Little is known on patterns of change over time in body composition, especially lean body mass (LBM), during massive weight loss after Roux-en-Y gastric bypass (RYGB) in obese patients. We performed sequential measurements of total and regional body composition in patients after RYGB, and we compared a subsample of patients after surgery to a nonsurgical control group of similar age and body fatness. We used dual-energy X-ray absorptiometry (DXA) before and at 3, 6, and 12 months after RYGB in 42 obese women (before surgery: age 39.5 +/- 11.6 years; BMI 44.6 +/- 6.1 kg/m(2); mean +/- s.d.) and in 48 control obese women referred for nonsurgical weight management, before weight loss. During 1-year follow-up after RYGB, there was a continuous decrease in body weight (-36.0 +/- 12.5 kg at 1 year), total fat mass (FM) (-26.0 +/- 9.1 kg), as well as in trunk and appendicular FM. In contrast, the decrease in total LBM (-9.8 +/- 4.8 kg at 1 year), as well as in trunk and appendicular LBM, plateaued after 3-6 months. Rates of loss in weight, FM, and LBM were highest during the first 3-month period after RYGB (6.4 +/- 1.8, 4.1 +/- 1.7, and 2.3 +/- 1.2 kg/month, respectively), then decreased continuously for FM but plateaued for LBM. There was no evidence of a decrease in total, trunk, or appendicular LBM in weight-reduced subjects compared to the control group. In conclusion, follow-up of these obese women revealed a differential pattern of change in FM and LBM after RYGB. Despite an important loss in LBM, especially during the 3-6 months of initial period, LBM appears to be spared thereafter.

  18. Characterisation of various grape seed oils by volatile compounds, triacylglycerol composition, total phenols and antioxidant capacity.

    PubMed

    Bail, Stefanie; Stuebiger, Gerald; Krist, Sabine; Unterweger, Heidrun; Buchbauer, Gerhard

    2008-06-01

    Grape seed oil (Oleum vitis viniferae) representing a promising plant fat, mainly used for culinary and pharmaceutical purposes as well as for various technical applications, was subject of the present investigation. HS-SPME-GC-MS was applied to study volatile compounds in several seed oil samples from different grape oils. The triacylglycerol (TAG) composition of these oils was analyzed by MALDI-TOF-MS/MS. In addition the total phenol content and the antioxidant capacity (using TEAC) of these oils were determined. The headspace of virgin grape oils from white and red grapes was dominated by ethyl octanoate (up to 27.5% related to the total level of volatiles), ethylacetate (up to 25.0%), ethanol (up to 22.7%), acetic acid (up to 17.2%), ethyl hexanoate (up to 17.4%) and 3-methylbutanol (up to 11.0%). Triacylglycerol composition was found to be dominated by LLL (up to 41.8%), LLP (up to 24.3%), LLO (up to 16.3%) and LOO (up to 11.7%), followed by LOP (up to 9.3%) and LOS/OOO (up to 4.3%). Total phenol content ranged between 59μg/g and 115.5μg/g GAE. Antioxidant capacity (TEAC) was analyzed to range between 0.09μg/g and 1.16μg/g. Copyright © 2007 Elsevier Ltd. All rights reserved.

  19. [Epiphytic communities of arboreal formations in Southern Vietnam: an analysis of species composition and synusias structure in dependence on the extent of anthropogenic impact].

    PubMed

    Es'kov, A K

    2013-01-01

    Species composition of epiphytic communities within different formations of Phú Quôc Island (Southern Vietnam) is studied. The dependence of species composition and structural complexity of epiphytic communities on formation quality is demonstrated. Representatives of different families differ notably in their sensitivity to disturbances. Most vulnerable are Orchidaceae which represent the dominant group in epiphytic community of rain forest and which drop out almost completely under anthropogenic impacts. In less disturbed forests, epiphyte species diversity increases mainly at the expense of "lower" synusias and directly depends on the formation layering. Diminishing of layering numbers leads to dropping out of species belonging to "lower" synusias. Among epiphytes, the indicators of disturbed communities can be detected, namely species of ruderal strategy (explerents). In primal rain forest, they are absent or barely noticeable. An index is proposed for estimation of epiphytic communitiy complexity.

  20. Impact of dietary fiber energy on the calculation of food total energy value in the Brazilian Food Composition Database.

    PubMed

    Menezes, Elizabete Wenzel de; Grande, Fernanda; Giuntini, Eliana Bistriche; Lopes, Tássia do Vale Cardoso; Dan, Milana Cara Tanasov; Prado, Samira Bernardino Ramos do; Franco, Bernadette Dora Gombossy de Melo; Charrondière, U Ruth; Lajolo, Franco Maria

    2016-02-15

    Dietary fiber (DF) contributes to the energy value of foods and including it in the calculation of total food energy has been recommended for food composition databases. The present study aimed to investigate the impact of including energy provided by the DF fermentation in the calculation of food energy. Total energy values of 1753 foods from the Brazilian Food Composition Database were calculated with or without the inclusion of DF energy. The energy values were compared, through the use of percentage difference (D%), in individual foods and in daily menus. Appreciable energy D% (⩾10) was observed in 321 foods, mainly in the group of vegetables, legumes and fruits. However, in the Brazilian typical menus containing foods from all groups, only D%<3 was observed. In mixed diets, the DF energy may cause slight variations in total energy; on the other hand, there is appreciable energy D% for certain foods, when individually considered. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Monitoring of Water Quality and Microalgae Species Composition of Penaeus monodon Ponds in Pulau Pinang, Malaysia

    PubMed Central

    Shaari, Asma Liyana; Surif, Misni; Latiff, Faazaz Abd.; Omar, Wan Maznah Wan; Ahmad, Mohd Noor

    2011-01-01

    Many reports have revealed that the abundance of microalgae in shrimp ponds vary with changes in environmental factors such as light, temperature, pH, salinity and nutrient level throughout a shrimp culture period. In this study, shrimp cultivation period was divided into three stages (initial = week 0–5, mid = week 6–10 and final = week 11–15). Physical and chemical parameters throughout the cultivation period were studied and species composition of microalgae was monitored. Physical parameters were found to fluctuate widely with light intensity ranging between 182.23–1278 μmol photon m−2s−1, temperature between 29.56°C −31.59°C, dissolved oxygen (DO) between 4.56–8.21 mg/l, pH between 7.65–8.49 and salinity between 20‰–30‰. Ammonium (NH4+-N), nitrite (NO2−-N), nitrate (NO3−-N), and orthophosphate (PO43−-P) concentrations in the pond at all cultivation stages ranged from 0.017 to 0.38 mg/l, 0.24 to 2.12 mg/l, 0.06 to 0.98 mg/l and 0.16 to 1.93 mg/l respectively. Statistical test (ANOVA) showed that there were no significant difference (p<0.05) in nutrients concentrations among the cultivation stages. All nutrients concentrations however were still in the tolerable level and safe for shrimp culture. The chlorophyll a contents were found to range from 5.03±2.17 to 32.61±0.35 μg/l throughout the cultivation period. A total of 19 microalgae species were found in the shrimp pond, with diatoms contributing up to 72% of the species followed by Chlorophyta (11%) and Cyanophyta (11%). However, weekly species abundance varied through the study period. At the initial stage, when there were no shrimps in the pond, Anabaena spp. and Oscillatoria spp. (Cyanophyta) were the dominant species, followed by Chlorella sp. and Dunaliella sp. (Chlorophyta). When shrimps were introduced into the pond, Amphora sp., Navicula sp. Gyrosigma sp. and Nitzschia sp. (diatoms) started to exist. At the middle and towards the final stage of the shrimp culture

  2. Chemical composition of particles from traditional burning of Pakistani wood species

    NASA Astrophysics Data System (ADS)

    Shahid, Imran; Kistler, Magdalena; Mukhtar, Azam; Ramirez-Santa Cruz, Carlos; Bauer, Heidi; Puxbaum, Hans

    2015-11-01

    Total particulate matter (TPM) emitted during burning of three types of Pakistani wood (eucalyptus camaldulensis, local name Safeeda; acacia nilotica, local name Kikar, Babul; dalbergia sissoo, Shisham, Tali) in a traditional brick stove were collected and analyzed for anhydrosugars, sugar alcohols, trace metals, soluble ions and carbonaceous species. This is a first study reporting anhydrosugars in wood smoke particles emitted during traditional burning of common wood types in Pakistan. Carbonaceous species showed the highest contribution to the particulate matter. Although the total carbon (TC) contribution was similar for all burnings (64.8-70.2%), the EC/OC ratio varied significantly, from 0.2 to 0.3 for Accacia and Dalbergia to 0.7-0.8 for Eucalyptus and Wood-mix. Among inorganic constituents potassium chloride and silicon were found at levels higher than 1%. The levoglucosan concentrations ranged from 3.0 to 6.6% (average 5.6%) with the highest value for Accacia and lowest value for the wood-mix. The high levoglucosan/mannosan ratios of 20-28 were typical for hardwood. The ratio between levoglucosan and galactosan varied stronger and was found to be around 13-20 for Accacia, Eucalyptus and Wood mix, and 43 for Dalbergia. The determined levoglucosan concentrations allowed assessing the conversion factor for calculation of biomass smoke contribution to ambient particulate matter levels in Pakistan.

  3. The biological activities and chemical composition of Pereskia species (Cactaceae)--a review.

    PubMed

    Pinto, Nícolas de Castro Campos; Scio, Elita

    2014-09-01

    The exploration of nature as a source of sustainable, novel bioactive substances continues to grow as natural products play a significant role in the search for new therapeutic and agricultural agents. In this context, plants of the genus Pereskia (Cactaceae) have been studied for their biological activities, and are evolving as an interesting subject in the search for new, bioactive compounds. These species are commonly used as human foodstuffs and in traditional medicine to treat a variety of diseases. This review focuses on the bioactivity and chemical composition of the genus Pereskia, and aims to stimulate further studies on the chemistry and biological potential of the genus.

  4. Edible Neotropical Blueberries: Antioxidant and Compositional Fingerprint Analysis

    PubMed Central

    DASTMALCHI, KEYVAN; FLORES, GEMA; PETROVA, VANYA; PEDRAZA-PEÑALOSA, PAOLA; KENNELLY, EDWARD J.

    2012-01-01

    Edible blueberry species are well recognized for their potential health benefits. Ericaceae fruits including the North American highbush blueberry (Vaccinium corymbosum L.) and five less common edible blueberry relatives from the New World tropics, Anthopterus wardii Ball, Cavendishia grandifolia Hoerld, Macleania coccoloboides A. C. Sm., Sphyrospermum buxifolium Poepp. & Endl., and Sphyrospermum cordifolium Benth, were investigated for their antioxidant properties and phenolic profiles. The Neotropical berries C. grandifolia and A. wardii exhibited significantly higher DPPH• and ABTS•+ scavenging and iron chelation activities than V. corymbosum. Total phenolic content and HPLC-PDA compositional fingerprint analyses were also carried out. Significant correlations were observed among total phenolic contents, DPPH• and ABTS•+ scavenging, and iron chelation activities. Using HPLC-PDA, the phenolic constituents in the berries were identified as chlorogenic acid, p-coumaric acid, hyperoside, quercetin-3-O-glucoside, isoorientin, isovitexin, orientin and vitexin. Principal components analysis reduced the dimensions of antioxidant and total phenolic data to two components, which accounted for 95% of total variation among the six fruits. Each fruit species formed its own cluster, and therefore the antioxidant profile of each species was shown to be distinct. PMID:21391608

  5. Effect of 6 weeks of whole body vibration training on total and segmental body composition in healthy young adults.

    PubMed

    Rubio-Arias, J A; Esteban, P; Martínez, F; Ramos-Campo, D J; Mendizábal, S; Berdejo-Del-Fresno, D; Jiménez-Díaz, J F

    2015-12-01

    The applied use of new technologies to enhance performance and improve health has been increasing. Initially, whole body vibration training (WBVT) was used as system to improve elite athlete performance. However, this is also used to improve body composition, especially there is a great attention on the effectiveness of WBVT to reduce fat and body weight, with a potential increase in muscle tissue. The aim of this study was to investigate the effects of a 6-week vibration-training program on total and segmental body composition in a group of physically healthy participants. The final study sample included 64 healthy young adults. Subjects were randomly allocated into the control group (CG: n = 26; 16 males and 10 females) and the experimental group (EGWBVT: n = 38; 19 males and 19 females). The program lasted six weeks with a frequency of three sessions per week and each session varied in intensity. There were not found statistically significant differences in any of the body composition variables analysed. This study suggests that a six-week vibration-training program with an increasing intensity (7.2 g-32.6 g) in healthy young adults that are not overweight did not alter total and segmental body composition.

  6. Species composition of by-catch from milkfish (Chanos chanos) fry fishery in selected sites in the Philippines as determined by DNA barcodes.

    PubMed

    Asis, Angelli Marie Jacynth M; Destura, Irma; Santos, Mudjekeewis D

    2016-05-01

    Milkfish fry fishery, an important industry in the Philippines, uses non-selective fishing gears and push nets in coastal areas which lead to the capture of other non-targeted juvenile aquatic species. Unfortunately, information on the amount and the identity of by-catch species is lacking thus the extent of impact of the fry fishery is not known. In this study, the species composition of milkfish fry fishery by-catch sampled from selected coastal areas that are known to be fry collection sites in the country were identified and assessed through the use of DNA barcoding. Analyses revealed that by-catch fish species of the milkfish fry industry included Black Tiger shrimp (Peneaus monodon), Tarpon (Megalops cyprinoides), Glass perchlets (Ambasis gymnocephalus and Ambasis buruensis), Ladyfish (Elops hawaiensis), Snapper (Lutjanus fulviflamma), Cardinal fishes (Apogon hyalosoma and Sphaeramia orbicularis), Whipfin siver biddy (Gerres filamentosus), Mullet (Liza sp.), Anchovy (Encrasicholina heteroloba), and Tiger perch (Terapon jarbua), almost all of which are potential marketable food fish and culture species. The results of the study provide preliminary information, as well as awareness, on the species composition of milkfish fry by-catch.

  7. Carotenoid composition of the flowers of Mimulus lewisii and related species: Implications regarding the prevalence and origin of two unique, allenic pigments.

    PubMed

    LaFountain, Amy M; Frank, Harry A; Yuan, Yao-Wu

    2015-05-01

    The genus Mimulus has been used as a model system in a wide range of ecological and evolutionary studies and contains many species with carotenoid pigmented flowers. However, the detailed carotenoid composition of these flowers has never been reported. In this paper the floral carotenoid composition of 11 Mimulus species are characterized using high-performance liquid chromatography, mass spectrometry and chemical methods with a particular focus on the genetic model species, Mimulus lewisii. M. lewisii flowers have five major carotenoids: antheraxanthin, violaxanthin, neoxanthin, and the unique allenic carotenoids, deepoxyneoxanthin and mimulaxanthin. This carotenoid profile is consistent with the expression levels of putative carotenoid biosynthetic genes in the M. lewisii flower. The other 10 species possess the same five carotenoids or a subset of these. Comparison of the carotenoid profiles among species in a phylogenetic context provides new insights into the biosynthesis and evolution of deepoxyneoxanthin and mimulaxanthin. This work also lays the foundation for future studies regarding transcriptional control of the carotenoid biosynthesis pathway in Mimulus flowers. Copyright © 2015 Elsevier Inc. All rights reserved.

  8. Walker Branch Throughfall Displacement Experiment Data Report: Site Characterization, System Performance, Weather, Species Composition, and Growth (NDP-078 and 078A)

    DOE Data Explorer

    Cushman, Robert M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States), Carbon Dioxide Information Analysis Center, Environmental Sciences Division; Hanson, Paul J. [Oak Ridge National Laboratory, Oak Ridge, TN (USA), Environmental Sciences Division; Todd, Donald E. [Oak Ridge National Laboratory, Oak Ridge, TN (USA), Environmental Sciences Division; Riggs, Jeffery S. [Oak Ridge National Laboratory, Oak Ridge, TN (USA), Instrumentation and Controls Division; Wolfe, Mark E. [Tennessee Valley Authority, Norris, TN (USA); O'Neill, Elizabeth G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States), Environmental Sciences Division

    2001-07-01

    This numeric data package provides data sets, and accompanying documentation, on site characterization, system performance, weather, species composition, and growth for the Throughfall Displacement Experiment, which was established in the Walker Branch Watershed of East Tennessee to provide data on the responses of forests to altered precipitation regimes. The specific data sets include soil water content and potential, coarse fraction of the soil profile, litter layer temperature, soil temperature, monthly weather, daily weather, hourly weather, species composition of trees and saplings, mature tree and sapling annual growth, and relative leaf area index. Fortran and SAS(TM) access codes are provided to read the ASCII data files.

  9. Floral nectary, nectar production dynamics and chemical composition in five nocturnal Oenothera species (Onagraceae) in relation to floral visitors.

    PubMed

    Antoń, Sebastian; Komoń-Janczara, Elwira; Denisow, Bożena

    2017-12-01

    Main conclusion The floral nectars were sucrose-dominant; however, nectar protein and amino acid contents differed, indicating that composition of nitrogenous compounds may vary considerably even between closely related plant species, irrespectively of nectary structure. Numerous zoophilous plants attract their pollinators by offering floral nectar; an aqueous solution produced by specialized secretory tissues, known as floral nectaries. Although many papers on nectaries and nectar already exist, there has been a little research into the structure of nectaries and/or nectar production and composition in species belonging to the same genus. To redress this imbalance, we sought, in the present paper, to describe the floral nectary, nectar production, and nectar composition in five nocturnal Oenothera species with respect to their floral visitors. The structure of nectaries was similar for all the species investigated, and comprised the epidermis (with nectarostomata), numerous layers of nectary parenchyma, and subsecretory parenchyma. Anthesis for a single flower was short (ca. 10-12 h), and flowers lasted only one night. The release of floral nectar commenced at the bud stage (approx. 4 h before anthesis) and nectar was available to pollinators until petal closure. Nectar concentration was relatively low (ca. 27%) and the nectar was sucrose-dominant, and composed mainly of sucrose, glucose and fructose. The protein content of the nectar was also relatively low (on average, 0.31 µg ml -1 ). Nevertheless, a great variety of amino acids, including both protein and non-protein types, was detected in the nectar profile of the investigated taxa. We noted both diurnal and nocturnal generalist, opportunistic floral insect visitors.

  10. A Pine Is a Pine and a Spruce Is a Spruce – The Effect of Tree Species and Stand Age on Epiphytic Lichen Communities

    PubMed Central

    Bäcklund, Sofia; Jönsson, Mari; Strengbom, Joachim; Frisch, Andreas; Thor, Göran

    2016-01-01

    With an increasing demand for forest-based products, there is a growing interest in introducing fast-growing non-native tree species in forest management. Such introductions often have unknown consequences for native forest biodiversity. In this study, we examine epiphytic lichen species richness and species composition on the trunks of non-native Pinus contorta and compare these to the native Pinus sylvestris and Picea abies in managed boreal forests in northern Sweden across a chronosequence of age classes. Overall, we recorded a total of 66,209 lichen occurrences belonging to 57 species in the 96 studied forest stands. We found no difference in species richness of lichens between stands of P. contorta and P. sylvestris, but stands of P. abies had higher total species richness. However, species richness of lichens in stands of P. abies decreased with increasing stand age, while no such age effect was detected for P. contorta and P. sylvestris. Lichen species composition progressively diverged with increasing stand age, and in 30-year-old stands all three tree species showed species-specific assemblages. Epiphytic lichen assemblages in stands of 30-year-old P. contorta were influenced by greater basal area, canopy closure, and average diameter at breast height, P. abies stands by higher branch density and canopy closure, and stands of P. sylvestris by greater bark crevice depth. Differences in lichen species richness and composition were mainly explained by canopy closure and habitat availability, and the greater canopy closure in mature P. abies stands promoted the colonization and growth of calicioid lichen species. Our results indicate that the non-native P. contorta have similar species richness as the native P. sylvestris. The main difference in lichen species richness and composition is between P. abies and Pinus spp. in managed forests of boreal Sweden. PMID:26799558

  11. Attenuated total reflectance spectroscopy of plant leaves: A tool for ecological and botanical studies

    USGS Publications Warehouse

    Ribeiro da Luz, B.

    2006-01-01

    ??? Attenuated total reflectance (ATR) spectra of plant leaves display complex absorption features related to organic constituents of leaf surfaces. The spectra can be recorded rapidly, both in the field and in the laboratory, without special sample preparation. ??? This paper explores sources of ATR spectral variation in leaves, including compositional, positional and temporal variations. Interspecific variations are also examined, including the use of ATR spectra as a tool for species identification. ??? Positional spectral variations generally reflected the abundance of cutin and the epicuticular wax thickness and composition. For example, leaves exposed to full sunlight commonly showed more prominent cutin- and wax-related absorption features compared with shaded leaves. Adaxial vs. abaxial leaf surfaces displayed spectral variations reflecting differences in trichome abundance and wax composition. Mature vs. young leaves showed changes in absorption band position and intensity related to cutin, polysaccharide, and possibly amorphous silica development on and near the leaf surfaces. ??? Provided that similar samples are compared (e.g. adaxial surfaces of mature, sun-exposed leaves) same-species individuals display practically identical ATR spectra. Using spectral matching procedures to analyze an ATR database containing 117 individuals, including 32 different tree species, 83% of the individuals were correctly identified. ?? The Authors (2006).

  12. Bat community species richness and composition in a restinga protected area in Southeastern Brazil.

    PubMed

    Oprea, M; Esbérard, C E L; Vieira, T B; Mendes, P; Pimenta, V T; Brito, D; Ditchfield, A D

    2009-11-01

    In Brazil, restingas are under severe human-induced impacts resulting in habitat degradation and loss and remain one of the less frequently studied ecosystems. The main objectives of the present study are to describe the bat community in a restinga in Paulo Cesar Vinha State Park, Guarapari municipality, state of Espírito Santo, southeastern Brazil. Fieldwork was conducted twice a month from August 2004 to September 2005. A total sampling effort of 40,300 m(2)/h, represents the largest sampling effort for sampling bats in restingas to date. Bats were sampled in five different vegetational types in the area. Captured bats were processed recording information on species, sex, age, forearm length and weight. Shannon Diversity and Jaccard indexes were used to analyse diversity and similarity among habitats in the Park. A total of 554 captures belonging to 14 species and two families were obtained. Noctilio leporinus was recorded through direct observation and an ultra-sound detector also registered the presence of individuals from the family Molossidae, without being possible to distinguish it at specific level. Frugivores were the most representative guild. Richness was higher in Clusia shrubs (11 species) and Caraís lagoon (10 species). Shannon diversity index was estimated at H' = 1.43 for the overall sample, with Caraís lagoon representing the most diverse habitat (H' = 1.60). The greater similarity (J = 0.714) was observed for the two areas under high human influence.

  13. Validity of total and segmental impedance measurements for prediction of body composition across ethnic population groups.

    PubMed

    Deurenberg, P; Deurenberg-Yap, M; Schouten, F J M

    2002-03-01

    To test the impact of body build factors on the validity of impedance-based body composition predictions across (ethnic) population groups and to study the suitability of segmental impedance measurements. Cross-sectional observational study. Ministry of Health and School of Physical Education, Nanyang Technological University, Singapore. A total of 291 female and male Chinese, Malays and Indian Singaporeans, aged 18-69, body mass index (BMI) 16.0-40.2 kg/ m2. Anthropometric parameters were measured in addition to impedance (100 kHz) of the total body, arms and legs. Impedance indexes were calculated as height2/impedance. Arm length (span) and leg length (sitting height), wrist and knee width were measured from which body build indices were calculated. Total body water (TBW) was measured using deuterium oxide dilution. Extra cellular water (ECW) was measured using bromide dilution. Body fat percentage was determined using a chemical four-compartment model. The bias of TBW predicted from total body impedance index (bias: measured minus predicted TBW) was different among the three ethnic groups, TBW being significantly underestimated in Indians compared to Chinese and Malays. This bias was found to be dependent on body water distribution (ECW/TBW) and parameters of body build, mainly relative (to height) arm length. After correcting for differences in body water distribution and body build parameters the differences in bias across the ethnic groups disappeared. The impedance index using total body impedance was better correlated with TBW than the impedance index of arm or leg impedance, even after corrections for body build parameters. The study shows that ethnic-specific bias of impedance-based prediction formulas for body composition is due mainly to differences in body build among the ethnic groups. This means that the use of 'general' prediction equations across different (ethnic) population groups without prior testing of their validity should be avoided. Total

  14. Influence of Lipid Composition in Amplifying or Ameliorating Toxicant Effects on Phytoplankton.

    DTIC Science & Technology

    1992-04-30

    since they often have a high lipid content and high concentrations of eicosapentaenoic acid (Sicko-Goad et al. 1988; Volkman et al. 1989; Ahlgren et al...in percent composition of total saturated and unsaturated fatty acids with respect to sampling period in the light/dark cycle .................. A2-3...diatom species ................ A1-4 A2 1 Fatty acid identification and percent composition with standard errors of all Cyclotella meneghiniana

  15. Composite depth dose measurement for total skin electron (TSE) treatments using radiochromic film

    NASA Astrophysics Data System (ADS)

    Gamble, Lisa M.; Farrell, Thomas J.; Jones, Glenn W.; Hayward, Joseph E.

    2003-04-01

    Total skin electron (TSE) radiotherapy is routinely used to treat cutaneous T-cell lymphomas and can be implemented using a modified Stanford technique. In our centre, the composite depth dose for this technique is achieved by a combination of two patient positions per day over a three-day cycle, and two gantry angles per patient position. Due to patient morphology, underdosed regions typically occur and have historically been measured using multiple thermoluminescent dosimeters (TLDs). We show that radiochromic film can be used as a two-dimensional relative dosimeter to measure the percent depth dose in TSE radiotherapy. Composite depth dose curves were measured in a cylindrical, polystyrene phantom and compared with TLD data. Both multiple films (1 film per day) and a single film were used in order to reproduce a realistic clinical scenario. First, three individual films were used to measure the depth dose, one per treatment day, and then compared with TLD data; this comparison showed a reasonable agreement. Secondly, a single film was used to measure the dose delivered over three daily treatments and then compared with TLD data; this comparison showed good agreement throughout the depth dose, which includes doses well below 1 Gy. It will be shown that one piece of radiochromic film is sufficient to measure the composite percent depth dose for a TSE beam, hence making radiochromic film a suitable candidate for monitoring underdosed patient regions.

  16. Composite depth dose measurement for total skin electron (TSE) treatments using radiochromic film.

    PubMed

    Gamble, Lisa M; Farrell, Thomas J; Jones, Glenn W; Hayward, Joseph E

    2003-04-07

    Total skin electron (TSE) radiotherapy is routinely used to treat cutaneous T-cell lymphomas and can be implemented using a modified Stanford technique. In our centre, the composite depth dose for this technique is achieved by a combination of two patient positions per day over a three-day cycle, and two gantry angles per patient position. Due to patient morphology, underdosed regions typically occur and have historically been measured using multiple thermoluminescent dosimeters (TLDs). We show that radiochromic film can be used as a two-dimensional relative dosimeter to measure the percent depth dose in TSE radiotherapy. Composite depth dose curves were measured in a cylindrical, polystyrene phantom and compared with TLD data. Both multiple films (1 film per day) and a single film were used in order to reproduce a realistic clinical scenario. First, three individual films were used to measure the depth dose, one per treatment day, and then compared with TLD data; this comparison showed a reasonable agreement. Secondly, a single film was used to measure the dose delivered over three daily treatments and then compared with TLD data; this comparison showed good agreement throughout the depth dose, which includes doses well below 1 Gy. It will be shown that one piece of radiochromic film is sufficient to measure the composite percent depth dose for a TSE beam, hence making radiochromic film a suitable candidate for monitoring underdosed patient regions.

  17. Assessing Historical Fish Community Composition Using Surveys, Historical Collection Data, and Species Distribution Models

    PubMed Central

    Labay, Ben; Cohen, Adam E.; Sissel, Blake; Hendrickson, Dean A.; Martin, F. Douglas; Sarkar, Sahotra

    2011-01-01

    Accurate establishment of baseline conditions is critical to successful management and habitat restoration. We demonstrate the ability to robustly estimate historical fish community composition and assess the current status of the urbanized Barton Creek watershed in central Texas, U.S.A. Fish species were surveyed in 2008 and the resulting data compared to three sources of fish occurrence information: (i) historical records from a museum specimen database and literature searches; (ii) a nearly identical survey conducted 15 years earlier; and (iii) a modeled historical community constructed with species distribution models (SDMs). This holistic approach, and especially the application of SDMs, allowed us to discover that the fish community in Barton Creek was more diverse than the historical data and survey methods alone indicated. Sixteen native species with high modeled probability of occurrence within the watershed were not found in the 2008 survey, seven of these were not found in either survey or in any of the historical collection records. Our approach allowed us to more rigorously establish the true baseline for the pre-development fish fauna and then to more accurately assess trends and develop hypotheses regarding factors driving current fish community composition to better inform management decisions and future restoration efforts. Smaller, urbanized freshwater systems, like Barton Creek, typically have a relatively poor historical biodiversity inventory coupled with long histories of alteration, and thus there is a propensity for land managers and researchers to apply inaccurate baseline standards. Our methods provide a way around that limitation by using SDMs derived from larger and richer biodiversity databases of a broader geographic scope. Broadly applied, we propose that this technique has potential to overcome limitations of popular bioassessment metrics (e.g., IBI) to become a versatile and robust management tool for determining status of

  18. Assessing historical fish community composition using surveys, historical collection data, and species distribution models.

    PubMed

    Labay, Ben; Cohen, Adam E; Sissel, Blake; Hendrickson, Dean A; Martin, F Douglas; Sarkar, Sahotra

    2011-01-01

    Accurate establishment of baseline conditions is critical to successful management and habitat restoration. We demonstrate the ability to robustly estimate historical fish community composition and assess the current status of the urbanized Barton Creek watershed in central Texas, U.S.A. Fish species were surveyed in 2008 and the resulting data compared to three sources of fish occurrence information: (i) historical records from a museum specimen database and literature searches; (ii) a nearly identical survey conducted 15 years earlier; and (iii) a modeled historical community constructed with species distribution models (SDMs). This holistic approach, and especially the application of SDMs, allowed us to discover that the fish community in Barton Creek was more diverse than the historical data and survey methods alone indicated. Sixteen native species with high modeled probability of occurrence within the watershed were not found in the 2008 survey, seven of these were not found in either survey or in any of the historical collection records. Our approach allowed us to more rigorously establish the true baseline for the pre-development fish fauna and then to more accurately assess trends and develop hypotheses regarding factors driving current fish community composition to better inform management decisions and future restoration efforts. Smaller, urbanized freshwater systems, like Barton Creek, typically have a relatively poor historical biodiversity inventory coupled with long histories of alteration, and thus there is a propensity for land managers and researchers to apply inaccurate baseline standards. Our methods provide a way around that limitation by using SDMs derived from larger and richer biodiversity databases of a broader geographic scope. Broadly applied, we propose that this technique has potential to overcome limitations of popular bioassessment metrics (e.g., IBI) to become a versatile and robust management tool for determining status of

  19. [Seasonal variation of proximate composition in three commercially important species in the Gulf of Nicoya, Puntarenas, Costa Rica].

    PubMed

    Rodríguez, Cristian Fonseca; Solera, Fabián Chavarriá; Mejía-Arana, Fernando

    2013-03-01

    Nutritional value of seafood for human consumption is worldwide recognized. Some information have been generated in other countries, nevertheless, there is limited information describing the chemical composition of some fishery important species caught in the Gulf of Nicoya. For this reason, we studied the levels of proximal components of the edible parts (fresh) of three commercially important species. The meat samples of snook Centropomus unionesis, the shrimp Trachypenaeus byrdi and the bivalve Polymesoda radiata, were collected from the Puntarenas local fish market during the fishing season of February 2009 to January 2010. Proximate composition analysis was determined according to AOAC methodology, and evaluated the moisture content, and protein and lipid composition of shellfish meats. The results indicated that the moisture content ranged from 74.6-80.6g/100g for snook 76.9-80.0g/100g for shrimp and 77.9-89.5g/100g for green mussel. After the moisture, the protein was the most abundant chemical fraction (6.8 to 21g/100g) showing the highest values in February for the shrimp and green mussel, and December for snook. The largest fluctuations in the lipid content were found in the snook, ranging from 0.7g/100g to 5.6g/100g; the highest values in this fraction were found in shrimp, green mussel and snook, for July, February and April samples respectively. Considering these results, we concluded that fish and shrimp species studied are a good alternative for human consumption as a source of protein and low lipid content.

  20. Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals.

    PubMed

    Torres-Pérez, Juan L; Guild, Liane S; Armstrong, Roy A; Corredor, Jorge; Zuluaga-Montero, Anabella; Polanco, Ramón

    2015-01-01

    Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral's symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5-98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health.

  1. Effects of past burning frequency on plant species structure and composition in dry dipterocarp forest

    NASA Astrophysics Data System (ADS)

    Wanthongchai, Dr.; Bauhus, Prof.; Goldammer, Prof.

    2009-04-01

    Anthropogenic burning in dry dipterocarp forests (DDF) has become a common phenomenon throughout Thailand. It is feared that too frequent fires may affect vegetation structure and composition and thus impact on ecosystem productivity. The aim of this study was to quantify the effects of prescribed fires on sites with different past burning regimes on vegetation structure and composition in the Huay Kha Khaeng Wildlife Sanctuary (HKK), Thailand. Fire frequency was determined from satellite images and ranged from frequent, infrequent, rare and unburned with fire occurrences of 7, 2, 1 and 0 out of the past 10 years, respectively. The pre-burn fuel loads, the overstorey and understorey vegetation structure and composition were determined to investigate the effects of the contrasting past burning regimes. The burning experiment was carried out, applying a three-strip head-fire burning technique. The vegetation structure and composition were sampled again one year after the fire to assess the fire impacts. Aboveground fine fuel loads increased with the length of fire-free interval. The woody plant structures of the frequently burned stand differed from those of the other less frequently burned stands. The species composition of the overstorey on the frequently burned site, in particular that of small sized trees (4.5-10 cm dbh), also differed significantly from that of the other sites. Whilst the ground vegetation including shrubs and herbs did not differ between the past burning regimes, frequent burning obviously promoted the proliferation of graminoid vegetation. There was no clear evidence showing that the prescribed fires affected the mortality of trees (dbh> 4.5 cm) on the sites of the different past burning regimes. The effects of prescribed burning on the understorey vegetation structures varied between the past burning regimes and the understorey vegetation type. Therefore, it is recommended that the DDF at HKK should be subjected to a prescribed fire frequency

  2. Designing mixed species tree plantations for the tropics: balancing ecological attributes of species with landholder preferences in the Philippines.

    PubMed

    Nguyen, Huong; Lamb, David; Herbohn, John; Firn, Jennifer

    2014-01-01

    A mixed species reforestation program known as the Rainforestation Farming system was undertaken in the Philippines to develop forms of farm forestry more suitable for smallholders than the simple monocultural plantations commonly used then. In this study, we describe the subsequent changes in stand structure and floristic composition of these plantations in order to learn from the experience and develop improved prescriptions for reforestation systems likely to be attractive to smallholders. We investigated stands aged from 6 to 11 years old on three successive occasions over a 6 year period. We found the number of species originally present in the plots as trees >5 cm dbh decreased from an initial total of 76 species to 65 species at the end of study period. But, at the same time, some new species reached the size class threshold and were recruited into the canopy layer. There was a substantial decline in tree density from an estimated stocking of about 5000 trees per ha at the time of planting to 1380 trees per ha at the time of the first measurement; the density declined by a further 4.9% per year. Changes in composition and stand structure were indicated by a marked shift in the Importance Value Index of species. Over six years, shade-intolerant species became less important and the native shade-tolerant species (often Dipterocarps) increased in importance. Based on how the Rainforestation Farming plantations developed in these early years, we suggest that mixed-species plantations elsewhere in the humid tropics should be around 1000 trees per ha or less, that the proportion of fast growing (and hence early maturing) trees should be about 30-40% of this initial density and that any fruit tree component should only be planted on the plantation margin where more light and space are available for crowns to develop.

  3. Designing Mixed Species Tree Plantations for the Tropics: Balancing Ecological Attributes of Species with Landholder Preferences in the Philippines

    PubMed Central

    Nguyen, Huong; Lamb, David; Herbohn, John; Firn, Jennifer

    2014-01-01

    A mixed species reforestation program known as the Rainforestation Farming system was undertaken in the Philippines to develop forms of farm forestry more suitable for smallholders than the simple monocultural plantations commonly used then. In this study, we describe the subsequent changes in stand structure and floristic composition of these plantations in order to learn from the experience and develop improved prescriptions for reforestation systems likely to be attractive to smallholders. We investigated stands aged from 6 to 11 years old on three successive occasions over a 6 year period. We found the number of species originally present in the plots as trees >5 cm dbh decreased from an initial total of 76 species to 65 species at the end of study period. But, at the same time, some new species reached the size class threshold and were recruited into the canopy layer. There was a substantial decline in tree density from an estimated stocking of about 5000 trees per ha at the time of planting to 1380 trees per ha at the time of the first measurement; the density declined by a further 4.9% per year. Changes in composition and stand structure were indicated by a marked shift in the Importance Value Index of species. Over six years, shade-intolerant species became less important and the native shade-tolerant species (often Dipterocarps) increased in importance. Based on how the Rainforestation Farming plantations developed in these early years, we suggest that mixed-species plantations elsewhere in the humid tropics should be around 1000 trees per ha or less, that the proportion of fast growing (and hence early maturing) trees should be about 30–40% of this initial density and that any fruit tree component should only be planted on the plantation margin where more light and space are available for crowns to develop. PMID:24751720

  4. Composition and toxigenic potential of the Fusarium graminearum species complex from maize ears, stalks and stubble in Brazil

    USDA-ARS?s Scientific Manuscript database

    Detailed knowledge of the composition and toxigenic potential of the Fusarium graminearum species complex affecting maize crops in Brazil is lacking. A multilocus genotype approach was used to identify 539 isolates from three sub-collections: 1) maize kernels (n= 110) from five states spanning sout...

  5. Herbaceous species composition and richness of mesophytic cove forests in the southern Appalachians: synthesis and knowledge gaps

    Treesearch

    Katherine J. Elliott; James M. Vose; Duke Rankin

    2014-01-01

    We synthesized the current information on mesophytic cove forests in the southern Appalachians, assessed the range of variation in herb species composition and diversity in stands with different disturbance histories and environmental conditions, identified key knowledge gaps, and suggested approaches to fill these knowledge gaps. The purpose of this synthesis was to...

  6. Linking environmental gradients, species composition, and vegetation indicators of sugar maple health in the northeastern United States

    Treesearch

    Stephen B. Horsley; Scott W. Bailey; Todd E. Ristau; Robert P. Long; Richard A. Hallett

    2008-01-01

    Sugar maple (Acer saccharum Marsh.) decline has occurred throughout its range over the past 50 years, although decline symptoms are minimal where nutritional thresholds of Ca, Mg, and Mn are met. Here, we show that availability of these elements also controls vascular plant species composition in northern hardwood stands and we identify indicator...

  7. Species composition, size structure, and disturbance: History of an old growth bottomland hardwood loblolly pine (Pinus taeda) forest in Arkansas, USA

    Treesearch

    Eric Heitzman; Michael G. Shelton; Adrian Grell

    2004-01-01

    The Lost Forty is a 16-ha old-growth bottomland hardwood-lobtolly pine (Pinus taeda L.) forest iocated in south-central Arkansas that has had little human disturbance. We established plots in the Lost Forty and collected data on species composition, tree size, age structure, and radial stem growth patterns. The overstory was dominated by species that...

  8. Fine-scale distribution of zooplankton is linked to phytoplankton species composition and abundance in a North Norwegian fjord system

    NASA Astrophysics Data System (ADS)

    Norrbin, F.; Priou, P. D.; Varela, A. P.

    2016-02-01

    We studied the influence of dense layers of phytoplankton and aggregates on shaping the vertical distribution of zooplankton in a North Norwegian fjord using a Video Plankton Recorder (VPR). This instrument provided fine-scale vertical distribution (cm-m scale) of planktonic organisms as well as aggregates of marine snow in relation to environmental conditions. At the height - later stage of the spring phytoplankton bloom in May, the outer part of the fjord was dominated by Phaeocystis pouchetii, while diatoms (Chaetoceros spp.) were dominating in the innermost basin. Small copepods species like Pseudocalanus spp., Microsetella norvegica, and Oithona spp. prevailed over larger copepod species in the inner part of the fjord whereas the outer part was dominated by large copepods like Calanus finmarchicus. While the zooplankton where spread out over the water column during the early stage of the bloom, in May they were linked to the phytoplankton vertical distribution and in the winter situation they were found in deeper waters. Herbivorous zooplankton species were affected by phytoplankton species composition; C. finmarchicus and Pseudocalanus spp. avoided the dense layer of P. pouchetii while herbivorous zooplankton matched the distribution of the diatom-dominated bloom. Small, omnivorous copepod species like Microsetella sp., Oithona sp. and Pseudocalanus sp. were often associated with dense layers of snow aggregates. This distribution may provide a shelter from predators as well as a food source. Natural or anthropogenic-induced changes in phytoplankton composition and aggregate distribution may thus influence food-web interactions.

  9. Proximate composition, amino acid and fatty acid composition of fish maws.

    PubMed

    Wen, Jing; Zeng, Ling; Xu, Youhou; Sun, Yulin; Chen, Ziming; Fan, Sigang

    2016-01-01

    Fish maws are commonly recommended and consumed in Asia over many centuries because it is believed to have some traditional medical properties. This study highlights and provides new information on the proximate composition, amino acid and fatty acid composition of fish maws of Cynoscion acoupa, Congresox talabonoides and Sciades proops. The results indicated that fish maws were excellent protein sources and low in fat content. The proteins in fish maws were rich in functional amino acids (FAAs) and the ratio of FAAs and total amino acids in fish maws ranged from 0.68 to 0.69. Among species, croaker C. acoupa contained the most polyunsaturated fatty acids, arachidonic acid, docosahexaenoic acid and eicosapntemacnioc acid, showing the lowest value of index of atherogenicity and index of thrombogenicity, showing the highest value of hypocholesterolemic/hypercholesterolemic ratio, which is the most desirable.

  10. Total and Segmental Body Composition Examination in Collegiate Football Players Using Multifrequency Bioelectrical Impedance Analysis and Dual X-ray Absorptiometry.

    PubMed

    Raymond, Christiana J; Dengel, Donald R; Bosch, Tyler A

    2018-03-01

    Raymond, CJ, Dengel, DR, and Bosch, TA. Total and segmental body composition examination in collegiate football players using multifrequency bioelectrical impedance analysis and dual X-ray absorptiometry. J Strength Cond Res 32(3): 772-782, 2018-The current study examined the influence of player position on the agreement between multifrequency bioelectrical impedance analysis (MfBIA) and dual X-ray absorptiometry (DXA) when assessing total and segmental percent body fat (BF%), fat mass (FM), and fat-free mass (FFM) in National Collegiate Athletic Association Division I collegiate football athletes. Forty-four male collegiate athletes (age = 19 ± 1 year; height = 1.9 ± 1.0 m; and body mass = 106.4 ± 18.8 kg) participated. Player positions included: offensive linemen (OL; n = 7), tight ends (TE; n = 4), wide receivers (WR; n = 9), defensive linemen (DL; n = 6), defensive backs (DB; n = 8), linebackers (LB; n = 6), and running backs (RB; n = 4). Total and segmental body composition measured using MfBIA were compared with values obtained using DXA. Compared with DXA, MfBIA underestimated BF% (3.0 ± 3.8%), total FM (2.5 ± 4.3 kg), arm FM (0.4 ± 0.8 kg), arm FFM (1.4 ± 0.9 kg), leg FM (2.8 ± 2.0 kg), and leg FFM (5.4 ± 2.4 kg) (all p < 0.001; arm FM p = 0.002) and overestimated total FFM (-2.4 ± 4.5 kg) (p < 0.001). Limits of agreement (LOAs) were: ±7.39% (BF%), ±8.50 kg (total FM), ±1.50 kg (arm FM), ±1.83 kg (arm FFM), ±3.83 kg (leg FM), ±4.62 kg (leg FFM), and ±8.83 kg (total FFM). No significant differences were observed between devices for trunk FM (-0.3 ± 3.0 kg; p = 0.565) and trunk FFM (0.4 ± 2.4 kg; p = 0.278), with LOAs of ±5.92 and ±4.69 kg, respectively. Player position significantly affected all between-device mean body composition measurement differences (adjusted p ≤ 0.05), with OL demonstrating the greatest effect on each variable. Therefore, MfBIA does not seem accurate in examining between-player body composition in college

  11. Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms

    NASA Astrophysics Data System (ADS)

    Liang, Ying; Mai, Kang-Sen; Sun, Shi-Chun; Yu, Dao-Zhan

    2001-09-01

    The effect of light intensity (1500 lx and 5000 lx) on the total lipid and fatty acid composition of six strains of marine diatoms Cylindrotheca fusiformis (B211), Phaeodactylum tricornutum (B114, B118 and B221) Nitzschia closterium (B222) and Chaetoceros gracilis (B13) was investigated. The total lipids of B13, B114, and B211 grown at 5000 lx were lower than those grown at 1500 lx. No evident changes were observed in B118, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14∶0, 16∶0, 16∶1 (n-7) and 20∶5(n-3). Cylindrotheca fusiformis had high percentage of 20∶4n 6(9.2 10.9%). The total polyunsaturated fatty acid in all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains except Chaetoceros gracilis.

  12. Managed forest landscape structure and avian species richness in the southeastern US

    Treesearch

    Craig Loehle; T. Bently Wigley; Scott Rutzmoser; John A. Gerwin; Patrick D. Keyser; Richard A. Lancia; Christopher J. Reynolds; Ronald E. Thill; Robert Weih; Don White; Petra Bohall Wood

    2005-01-01

    Forest structural features at the stand scale (e.g., snags, stem density, species composition) and habitat attributes at larger spatial scales (e.g., landscape pattern, road density) can influence biological diversity and have been proposed as indicators in sustainable forestry programs. This study investigated relationships between such factors and total richness of...

  13. High density polyethylene/graphite nano-composites for total hip joint replacements: processing and in vitro characterization.

    PubMed

    Fouad, H; Elleithy, Rabeh

    2011-10-01

    The main objective of the present study is to investigate how the thermal, rheological, mechanical and cytotoxicity behavior of High Density Polyethylene (HDPE) can be changed by the addition of graphite nano particles (GNPs) at different contents. The HDPE/GNPs composites were prepared using melt blending in a co-rotating intermeshing twin screw extruder. The in vitro tests results showed that the original material (HDPE) and all HDPE/GNPs composites do not exhibit any cytotoxicity to the WISH cell line. The microscopic examination of the nano-composite tensile-fractured surface found a good distribution of GNPs in the HDPE matrix. The Differential Scanning Calorimetry (DSC) results indicated that the crystallization percentage increased by adding GNPs to HDPE up to 4%. The XRD patterns of the HDPE/GNPs composites showed an increase in peak intensity compared to neat HDPE. This increase echoed the crystallinity results obtained from DSC. The rheological tests showed that the complex viscosity of the HDPE increased as the percentage of GNPs increased due to the restriction of the molecular mobility. The tensile test results showed that with increasing the GNPs content, Young's modulus and the yield strength of the HDPE/GNPs composite increased while the strain at fracture decreased. Finally, the preliminary results of the abrasion test indicated that the abrasion rate decreased by increasing the GNPs ratio up to 4% content. The prepared HDPE/GNPs composites appear to have fairly good comprehensive properties that make them a good candidate as a bearing material for the total joint replacement. Copyright © 2011 Elsevier Ltd. All rights reserved.

  14. Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India

    PubMed Central

    Subramanya, M. D.; Pai, Sandeep R.; Upadhya, Vinayak; Ankad, Gireesh M.; Bhagwat, Shalini S.; Hegde, Harsha V.

    2015-01-01

    Background: Sida L., is a medicinally important genus, the species of which are widely used in traditional systems of medicine in India. Pharmacologically, roots are known for anti-tumor, anti-HIV, hepatoprotective, and many other properties. Phenolic antioxidants help in reducing oxidative stress occurring during treatment of such diseases. Objective: The study aimed to evaluate and compare polyphenol contents and antioxidant properties of eight selected species of Sida from Western Ghats, India. Materials and Methods: Methanolic root extracts (10% w/v) of Sida species, viz., S. acuta, S. cordata, S. cordifolia, S. indica, S. mysorensis, S. retusa, S. rhombifolia, and S. spinosa were analyzed. Results: Sida cordifolia possessed highest total phenolic content (TPC: 1.92 ± 0.10 mg Caffeic Acid Equivalent/g and 2.13 ± 0.11 mg Tannic Acid Equivalant/g), total flavonoid content (TF: 2.60 ± 0.13 mg Quercetin Equivalent/g) and also possessed highest antioxidant activities in 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging (51.31 ± 2.57% Radical Scavenging Activity, (RSA); Trolox Equivalent Antioxidant Capacity: 566.25 ± 28.31μM; Ascorbic acid Equivalent Antioxidant Capacity: 477.80 ± 23.89 μM) and Ferric Reducing Antioxidant Power assays (TEAC: 590.67 ± 29.53 μM; AEAC: 600.67 ± 30.03 μM). Unlike DPPH and Ferric Reducing Antioxidant Power (FRAP) activity, 2, 2′-Azinobis (3-ethyl Benzo Thiazoline-6-Sulfonic acid) ABTS+ antioxidant activity was highest in S. indica (TEAC: 878.44 ± 43.92 μM; AEAC 968.44 ± 48.42 μM). It was significant to note that values of AEAC (μM) for all the antioxidant activities analyzed were higher than that of TEAC. Conclusion: The high contents of phenolic compounds in the root extracts of selected Sida species have direct correlation with their antioxidant properties. Conclusively, roots of S. cordifolia can be considered as the potential source of polyphenols and antioxidants. PMID:25878460

  15. Effects of species and season on chemical composition and ruminal crude protein and organic matter degradability of some multi-purpose tree species by West African dwarf rams.

    PubMed

    Arigbede, O M; Anele, U Y; Südekum, K-H; Hummel, J; Oni, A O; Olanite, J A; Isah, A O

    2012-04-01

    Seasonal chemical composition and ruminal organic matter (OM) and crude protein (CP) degradabilities were determined in four tropical multi-purpose tree species (MPTS) namely; Pterocarpus santalinoides, Grewia pubescens, Enterolobium cyclocarpum and Leucaena leucocephala. Three West African dwarf (WAD) rams fitted with permanent rumen cannula were used for the degradability trials. Foliage samples were collected four times to represent seasonal variations as follows: January--mid dry; April--late dry; July--mid rainy and October--late rainy seasons. Leaf samples were randomly collected from the trees for estimation of dry matter (DM) and chemical composition. Ruminal in sacco OM and CP degradabilities were estimated from residues in nylon bags. All samples had high CP (161-259 g/kg DM) and moderate fibre concentrations [neutral detergent fibre (without residual ash], 300-501 g/kg DM; acid detergent fibre (without residual ash), 225-409 g/kg DM and acid detergent lignin, 87-179 g/kg DM across seasons. Interaction effects of species and season on chemical composition were highly significant (p = 0.001) except for trypsin inhibitor (p = 0.614). The MPTS recorded more than 60% OM and CP degradability at 24 h, which implied that they were all highly degradable in the rumen. Their incorporation into ruminant feeding systems as dry season forage supplements is therefore recommended. © 2011 Blackwell Verlag GmbH.

  16. Fillet proximate composition, lipid quality, yields, and organoleptic quality of Mediterranean-farmed marine fish: A review with emphasis on new species.

    PubMed

    Grigorakis, Kriton

    2017-09-22

    Species diversification in Mediterranean mariculture involves various important fish that contribute to the diet of many human populations. These include meagres (Sciaenidae), flatfishes, mullets, and various sparids. Their quality aspects (yields, fillet proximate composition, and lipid quality) are discussed in this review. Their filleting yield is mostly 40-45%. The viscerosomatic index ranges from 1.5% to 14%, depending on species. Low muscle fat contents of flatfishes and meagres differentiate them from the rest of the farmed species. Farmed fish contain high n-3 polyunsaturates fatty acids (PUFA; 12.3-36.3% vs. 5.48-37.2% in the wild) and have higher muscle fat and n-6 PUFA contents (mainly 18:2 n-6) than their wild counterparts. The aquaculture management, diet, and season can affect fillet composition and fatty acids, while season (i.e. food availability and maturation) largely affects lipid quality in wild fish. Data on the sensory quality of Mediterranean-farmed species are mainly limited to whether specific management differentiates the sensory quality; thus, further development of tools for sensory analysis is required. Observations on the quality features in farmed Mediterranean fish indicate that species diversification can also provide product diversification based on different commercial weights and fillet quality specifications.

  17. Survey of duckweed diversity in Lake Chao and total fatty acid, triacylglycerol, profiles of representative strains.

    PubMed

    Tang, J; Li, Y; Ma, J; Cheng, J J

    2015-09-01

    Lemnaceae (duckweeds) are widely distributed aquatic flowering plants. Their high growth rate, starch content and suitability for bioremediation make them potential feedstock for biofuels. However, few natural duckweed resources have been investigated in China, and there is no information about total fatty acid (TFA) and triacylglycerol (TAG) composition of duckweeds from China. Here, the genetic diversity of a natural duckweed population collected from Lake Chao, China, was investigated using multilocus sequence typing (MLST). The 54 strains were categorised into four species in four genera, representing 12 distinct sequence types. Strains representing Lemna aequinoctialis and Spirodela polyrhiza were predominant. Interestingly, a surprisingly high degree of genetic diversification within L. aequinoctialis was observed. The four duckweed species revealed a uniform fatty acid composition, with three fatty acids, palmitic acid, linoleic acid and linolenic acid, accounting for more than 80% of the TFA. The TFA in biomass varied among species, ranging from 1.05% (of dry weight, DW) for L. punctata and S. polyrhiza to 1.62% for Wolffia globosa. The four duckweed species contained similar TAG contents, 0.02% mg · DW(-1). The fatty acid profiles of TAG were different from those of TFA, and also varied among the four species. The survey investigated the genetic diversity of duckweeds from Lake Chao, and provides an initial insight into TFA and TAG of four duckweed species, indicating that intraspecific and interspecific variations exist in the content and composition of both TFA and TAG in comparison with other studies. © 2015 German Botanical Society and The Royal Botanical Society of the Netherlands.

  18. A regional composite-face effect for species-specific recognition: Upper and lower halves play different roles in holistic processing of monkey faces.

    PubMed

    Wang, Zhe; Quinn, Paul C; Jin, Haiyang; Sun, Yu-Hao P; Tanaka, James W; Pascalis, Olivier; Lee, Kang

    2018-04-25

    Using a composite-face paradigm, we examined the holistic processing induced by Asian faces, Caucasian faces, and monkey faces with human Asian participants in two experiments. In Experiment 1, participants were asked to judge whether the upper halves of two faces successively presented were the same or different. A composite-face effect was found for Asian faces and Caucasian faces, but not for monkey faces. In Experiment 2, participants were asked to judge whether the lower halves of the two faces successively presented were the same or different. A composite-face effect was found for monkey faces as well as for Asian faces and Caucasian faces. Collectively, these results reveal that own-species (i.e., own-race and other-race) faces engage holistic processing in both upper and lower halves of the face, but other-species (i.e., monkey) faces engage holistic processing only when participants are asked to match the lower halves of the face. The findings are discussed in the context of a region-based holistic processing account for the species-specific effect in face recognition. Copyright © 2018 Elsevier Ltd. All rights reserved.

  19. Depth related trends in proximate composition of demersal fishes in the eastern North Pacific

    NASA Astrophysics Data System (ADS)

    Drazen, J. C.

    2007-02-01

    The proximate chemistry of the white muscle and liver of 18 species of demersal fish from the eastern North Pacific was studied to determine trends with depth, locomotory mode and buoyancy mechanism, foraging strategy and to elucidate energetic strategies. Data for 24 species from shallow water were taken from the literature and included for analysis of muscle water content. Benthopelagic species, primarily gadiforms, have significantly larger lipid-rich livers than benthic species. The benthopelagic species may use this lipid to add buoyancy, but it is also used as energy storage. Buoyancy mechanism was directly related to proximate composition. Fishes using gasbladders had normal muscle composition. The two species of benthopelagic fishes without gasbladders have either very high muscle lipid content ( Anoplopoma fimbria) or gelatinous muscle ( Alepocephalus tenobrosus) to aid in achieving neutral buoyancy. The macrourid, Albatrossia pectoralis, has a very small gasbladder and also has gelatinous muscle. Both of these benthopelagic fishes with gelatinous muscle feed on pelagic organisms. Gelatinous muscle was also found in two flatfishes that inhabit the oxygen minimum zone. For these fishes, high water content may serve to lower metabolic costs while maintaining large body size. Scavengers such as Coryphaenoides armatus and Coryphaenoides acrolepis have lipid rich livers and others such as A. fimbria and Pachycara sp. have high and variable muscle lipid content. Thus foraging mode also acts to influence proximate composition. Several depth-related trends in proximate composition were found. White muscle water content increased significantly with depth, and all four gelatinous species occurred at bathyal depths. This adds evidence in support of the hypothesis that decreasing light levels shorten reactive distances and relax the selective pressure for high locomotory capacity. In addition significant declines in liver protein content were observed, suggesting that

  20. Stink bug species composition and relative abundance of the redbanded stink bug (Hemiptera: Pentatomidae) in soybean in the upper gulf coast Texas.

    PubMed

    Vyavhare, Suhas S; Way, Michael O; Medina, Raul F

    2014-12-01

    Stink bugs are the primary arthropod soybean pests in the southern United States. Historically, important stink bug species damaging soybeans in the southern United States included the southern green stink bug Nezara viridula (L.), the green stink bug Chinavia hilaris (Say), and the brown stink bug Euschistus servus (Say) (Hemiptera: Pentatomidae). The redbanded stink bug, Piezodorus guildinii (Westwood), has recently become an economic pest of soybean in the southern region of the United States, especially in Louisiana and Texas. Little is known about current stink bug species composition and relative abundance in Texan soybean agro-ecosystems. To fill this gap, commercial soybean fields in the Upper Gulf Coast of Texas were sampled weekly during the growing season using a sweep net throughout R2 (full flowering) to R7 (beginning maturity) from 2011 to 2013. Adults and nymphs (third, fourth, and fifth instars) of redbanded stink bug, southern green stink bug, green stink bug, and brown stink bug were counted per 25 sweeps. The relative abundance of redbanded stink bug was significantly higher than any other stink bug species throughout 2011-2013. Over 65% of the total population of major stink bugs collected during this period were redbanded stink bugs and ≍19% were southern green stink bugs. The highest redbanded stink bug densities and the highest ratio of redbanded stink bug nymphs to adults were recorded at R7. Results from this study show that redbanded stink bug has become the predominant stink bug species in soybean in the Upper Gulf Coast of Texas.

  1. Compositional stability and diversity of vascular plant communities following logging disturbance in Appalachian forests.

    PubMed

    Belote, R Travis; Jones, Robert H; Wieboldt, Thomas F

    2012-03-01

    Human-caused changes in disturbance regimes and introductions of nonnative species have the potential to result in widespread, directional changes in forest community structure. The degree that plant community composition persists or changes following disturbances depends on the balance between local extirpation and colonization by new species, including nonnatives. In this study, we examined species losses and gains, and entry of native vs. exotic species to determine how oak forests in the Appalachian Mountains might shift in species composition following a gradient of pulse disturbances (timber harvesting). We asked (1) how compositional stability of the plant community (resistance and resilience) was influenced by disturbance intensity, (2) whether community responses were driven by extirpation or colonization of species, and (3) how disturbance intensity influenced total and functional group diversity, including the nonnative proportion of the flora through time. We collected data at three spatial scales and three times, including just before, one year post-disturbance, and 10 years post-disturbance. Resistance was estimated using community distance measures between pre- and one year post-disturbance, and resilience using community distance between pre- and 10-year post-disturbance conditions. The number of colonizing and extirpated species between sampling times was analyzed for all species combined and for six functional groups. Resistance and resilience decreased with increasing timber-harvesting disturbance; compositional stability was lower in the most disturbed plots, which was driven by colonization, but not extirpation, of species. Colonization of species also led to increases in diversity after disturbance that was typically maintained after 10 years following disturbance. Most of the community-level responses were driven by post-disturbance colonization of native forbs and graminoids. The nonnative proportion of plant species tended to increase

  2. Seed reserve composition in 19 tree species of a tropical deciduous forest in Mexico and its relationship to seed germination and seedling growth

    PubMed Central

    Soriano, Diana; Orozco-Segovia, Alma; Márquez-Guzmán, Judith; Kitajima, Kaoru; Gamboa-de Buen, Alicia; Huante, Pilar

    2011-01-01

    Background and Aims The size and composition of seed reserves may reflect the ecological strategy and evolutionary history of a species and also temporal variation in resource availability. The seed mass and composition of seed reserves of 19 co-existing tree species were studied, and we examined how they varied among species in relation to germination and seedling growth rates, as well as between two years with contrasting precipitation (652 and 384 mm). Methods Seeds were collected from a tropical deciduous forest in the northwest of Mexico (Chamela Biological Station). The seed dry mass, with and without the seed coat, and the concentrations of lipids, nitrogen and non-structural carbohydrates for the seed minus seed coat were determined. The anatomical localization of these reserves was examined using histochemical analysis. The germination capacity, rate and lag time were determined. The correlations among these variables, and their relationship to previously reported seedling relative growth rates, were evaluated with and without phylogenetic consideration. Key Results There were interannual differences in seed mass and reserve composition. Seed was significantly heavier after the drier year in five species. Nitrogen concentration was positively correlated with seed coat fraction, and was significantly higher after the drier year in 12 species. The rate and lag time of germination were negatively correlated with each other. These trait correlations were also supported for phylogenetic independent contrasts. Principal component analysis supported these correlations, and indicated a negative association of seedling relative growth rate with seed size, and a positive association of germination rate with nitrogen and lipid concentrations. Conclusions Nitrogen concentration tended to be higher after the drier year and, while interannual variations in seed size and reserve composition were not sufficient to affect interspecific correlations among seed and seedling

  3. Bioengineer effects on understory species richness, diversity, and composition change along an environmental stress gradient: Experimental and mensurative evidence

    NASA Astrophysics Data System (ADS)

    Watt, Cortney A.; Scrosati, Ricardo A.

    2013-05-01

    Canopy-forming bioengineer species are commonly assumed to increase local species richness and diversity. We tested this notion by investigating the effects of fucoid seaweed canopies on understory communities along rocky intertidal elevation gradients in Atlantic Canada. Such gradients exhibit increasing thermal extremes and variation from low to high elevations, and are broadly used in stress gradient studies. A manipulative experiment created canopy and no-canopy treatments at the low, middle, and high intertidal zones, eliminating all species (except fucoid canopies) from replicate quadrats. After recolonization, overall richness and diversity (considering all primary producers and consumers) were higher under canopies than uncovered by canopies at the high and middle zones, but no effects occurred at the low zone. Similarly, species composition was affected by canopies at the high and middle zones, but not at the low zone. A mensurative study that surveyed the full range of canopy cover (0-100%) using nearly five times more quadrats from pristine areas yielded the same results: richness and diversity increased with canopy cover at the high and middle zones (approaching stabilization toward high cover values), but no effects occurred at the low zone. Lack of canopy effects at low elevations is related to mild habitat conditions, which canopies are unable to modify, while positive effects at higher elevations relate to the capacity of canopies to ameliorate harsh conditions. This is the first time that a combined experimental and mensurative approach shows that the same bioengineer species affect overall species richness, diversity, and composition differently along a stress gradient. Overall, protecting canopy-forming bioengineers to preserve local biodiversity should be most effective in stressful environments.

  4. Predicting the digestible energy of corn determined with growing swine from nutrient composition and cross-species measurements.

    PubMed

    Smith, B; Hassen, A; Hinds, M; Rice, D; Jones, D; Sauber, T; Iiams, C; Sevenich, D; Allen, R; Owens, F; McNaughton, J; Parsons, C

    2015-03-01

    The DE values of corn grain for pigs will differ among corn sources. More accurate prediction of DE may improve diet formulation and reduce diet cost. Corn grain sources ( = 83) were assayed with growing swine (20 kg) in DE experiments with total collection of feces, with 3-wk-old broiler chick in nitrogen-corrected apparent ME (AME) trials and with cecectomized adult roosters in nitrogen-corrected true ME (TME) studies. Additional AME data for the corn grain source set was generated based on an existing near-infrared transmittance prediction model (near-infrared transmittance-predicted AME [NIT-AME]). Corn source nutrient composition was determined by wet chemistry methods. These data were then used to 1) test the accuracy of predicting swine DE of individual corn sources based on available literature equations and nutrient composition and 2) develop models for predicting DE of sources from nutrient composition and the cross-species information gathered above (AME, NIT-AME, and TME). The overall measured DE, AME, NIT-AME, and TME values were 4,105 ± 11, 4,006 ± 10, 4,004 ± 10, and 4,086 ± 12 kcal/kg DM, respectively. Prediction models were developed using 80% of the corn grain sources; the remaining 20% was reserved for validation of the developed prediction equation. Literature equations based on nutrient composition proved imprecise for predicting corn DE; the root mean square error of prediction ranged from 105 to 331 kcal/kg, an equivalent of 2.6 to 8.8% error. Yet among the corn composition traits, 4-variable models developed in the current study provided adequate prediction of DE (model ranging from 0.76 to 0.79 and root mean square error [RMSE] of 50 kcal/kg). When prediction equations were tested using the validation set, these models had a 1 to 1.2% error of prediction. Simple linear equations from AME, NIT-AME, or TME provided an accurate prediction of DE for individual sources ( ranged from 0.65 to 0.73 and RMSE ranged from 50 to 61 kcal

  5. Proximate composition and mineral contents in the body wall of two species of sea cucumber from Oman Sea.

    PubMed

    Barzkar, Noora; Attaran Fariman, Gilan; Taheri, Ali

    2017-08-01

    The proximate composition and mineral contents of Stichopus horrens and Holothuria arenicola from Chabahar Bay were analyzed and investigated. During the present study, we aimed to demonstrate the nutritive value. The approximate percent composition of moisture, protein, fat, and ash were 92.8, 3.47, 0.4, and 3.33% in S. horrens and 93, 4.4, 0.6, and 2% in H. arenicola, respectively. Atomic absorption spectrophotometry of the ashes indicated the body wall of two species of sea cucumbers contained higher amounts of both macro minerals (92.5 mg/100 g Mg in S. horrens and 115 mg/100 g Mg in H. arenicola; 106.25 mg/100 g Ca in S. horrens and 83.25 mg/100 g Ca in H. arenicola) and trace elements (521.781 mg/100 g Fe in S. horrens; 60.354 mg/100 g Fe in H. arenicola, and 0.096 mg/100 g Zn in S. horrens; 0.04 mg/100 g Zn in H. arenicola). For both species, there were high content of protein and essential mineral. Also, they have low content of fat in the body wall of two species in the experiment.

  6. Sylhet-Kopili/Barail-Tipam Composite Total Petroleum System, Assam Geologic Province, India

    USGS Publications Warehouse

    Wandrey, Craig J.

    2004-01-01

    The Sylhet-Kopili/Barail-Tipam Composite total petroleum system (TPS) (803401) is located in the Assam Province in northeasternmost India and includes the Assam Shelf south of the Brahmaputra River. The area is primarily a southeast-dipping shelf overthrust by the Naga Hills on the southeast and the Himalaya Mountain range to the north. The rocks that compose this TPS are those of the Sylhet-Kopili/Barail-Tipam composite petroleum system. These rocks are those of the Eocene-Oligocene Jaintia Group Sylhet and Kopili Formations, the Oligocene Barail Group, the Oligocene-Miocene Surma and Tipam Groups. These groups include platform carbonates, shallow marine shales and sandstones, and the sandstones, siltstones, shales, and coals of deltaic and lagoonal facies. Source rocks include the Sylhet and Kopili Formation shales, Barail Group coals and shales, and in the south the Surma Group shales. Total organic content is generally low, averaging from 0.5 to 1.8 percent; it is as high as 9 percent in the Barail Coal Shales. Maturities are generally low, from Ro 0.45 to 0.7 percent where sampled. Maturity increases to the southeast near the Naga thrust fault and can be expected to be higher in the subthrust. Generation began in early Pliocene. Migration is primarily updip to the northwest (< 5 to 15 kilometers) along the northeast-trending slope of the Assam Shelf, and vertical migration occurs through reactivated basement-rooted faults associated with the plate collisions. Reservoir rocks are carbonates of the Sylhet Formation, interbedded sandstones of the Kopili Formation and sandstones of the Barail, Surma, and Tipam Groups. Permeability ranges from less than 8 mD (millidarcies) to as high as 800 mD in the Tipam Group. Porosity ranges from less than 7 percent to 30 percent. Traps are primarily anticlines and faulted anticlines with a few subtle stratigraphic traps. There is also a likelihood of anticlinal traps in the subthrust. Seals include interbedded Oligocene and

  7. Gas composition of sludge residue profiles in a sludge treatment reed bed between loadings.

    PubMed

    Larsen, Julie D; Nielsen, Steen M; Scheutz, Charlotte

    2017-11-01

    Treatment of sludge in sludge treatment reed bed systems includes dewatering and mineralization. The mineralization process, which is driven by microorganisms, produces different gas species as by-products. The pore space composition of the gas species provides useful information on the biological processes occurring in the sludge residue. In this study, we measured the change in composition of gas species in the pore space at different depth levels in vertical sludge residue profiles during a resting period of 32 days. The gas composition of the pore space in the sludge residue changed during the resting period. As the resting period proceeded, atmospheric air re-entered the pore space at all depth levels. The methane (CH 4 ) concentration was at its highest during the first part of the resting period, and then declined as the sludge residue became more dewatered and thereby aerated. In the pore space, the concentration of CH 4 often exceeded the concentration of carbon dioxide (CO 2 ). However, the total emission of CO 2 from the surface of the sludge residue exceeded the total emission of CH 4 , suggesting that CO 2 was mainly produced in the layer of newly applied sludge and/or that CO 2 was emitted from the sludge residue more readily compared to CH 4 .

  8. Quantifying assemblage turnover and species contributions at ecologic boundaries.

    PubMed

    Hayek, Lee-Ann C; Wilson, Brent

    2013-01-01

    Not all boundaries, whether stratigraphical or geographical, are marked by species-level changes in community composition. For example, paleodata for some sites do not show readily discernible glacial-interglacial contrasts. Rather, the proportional abundances of species can vary subtly between glacials and interglacials. This paper presents a simple quantitative measure of assemblage turnover (assemblage turnover index, ATI) that uses changes in species' proportional abundances to identify intervals of community change. A second, functionally-related index (conditioned-on-boundary index, CoBI) identifies species contributions to the total assemblage turnover. With these measures we examine benthonic foraminiferal assemblages to assess glacial/interglacial contrasts at abyssal depths. Our results indicate that these measures, ATI and CoBI, have potential as sequence stratigraphic tools in abyssal depth deposits. Many peaks in the set of values of ATI coincide with terminations at the end of glaciations and delineate peak-bounded ATI intervals (PATIs) separated by boundaries that approximate to glacial terminations and to transgressions at neritic depths. These measures, however, can be used to evaluate the assemblage turnover and composition at any defined ecological or paleoecological boundary. The section used is from Ocean Drilling Program (OPD) Hole 994C, drilled on the Blake Ridge, offshore SE USA.

  9. Quantifying Assemblage Turnover and Species Contributions at Ecologic Boundaries

    PubMed Central

    Hayek, Lee-Ann C.; Wilson, Brent

    2013-01-01

    Not all boundaries, whether stratigraphical or geographical, are marked by species-level changes in community composition. For example, paleodata for some sites do not show readily discernible glacial-interglacial contrasts. Rather, the proportional abundances of species can vary subtly between glacials and interglacials. This paper presents a simple quantitative measure of assemblage turnover (assemblage turnover index, ATI) that uses changes in species' proportional abundances to identify intervals of community change. A second, functionally-related index (conditioned-on-boundary index, CoBI) identifies species contributions to the total assemblage turnover. With these measures we examine benthonic foraminiferal assemblages to assess glacial/interglacial contrasts at abyssal depths. Our results indicate that these measures, ATI and CoBI, have potential as sequence stratigraphic tools in abyssal depth deposits. Many peaks in the set of values of ATI coincide with terminations at the end of glaciations and delineate peak-bounded ATI intervals (PATIs) separated by boundaries that approximate to glacial terminations and to transgressions at neritic depths. These measures, however, can be used to evaluate the assemblage turnover and composition at any defined ecological or paleoecological boundary. The section used is from Ocean Drilling Program (OPD) Hole 994C, drilled on the Blake Ridge, offshore SE USA. PMID:24130679

  10. Species undersampling in tropical bat surveys: effects on emerging biodiversity patterns.

    PubMed

    Meyer, Christoph F J; Aguiar, Ludmilla M S; Aguirre, Luis F; Baumgarten, Julio; Clarke, Frank M; Cosson, Jean-François; Estrada Villegas, Sergio; Fahr, Jakob; Faria, Deborah; Furey, Neil; Henry, Mickaël; Jenkins, Richard K B; Kunz, Thomas H; Cristina MacSwiney González, M; Moya, Isabel; Pons, Jean-Marc; Racey, Paul A; Rex, Katja; Sampaio, Erica M; Stoner, Kathryn E; Voigt, Christian C; von Staden, Dietrich; Weise, Christa D; Kalko, Elisabeth K V

    2015-01-01

    Undersampling is commonplace in biodiversity surveys of species-rich tropical assemblages in which rare taxa abound, with possible repercussions for our ability to implement surveys and monitoring programmes in a cost-effective way. We investigated the consequences of information loss due to species undersampling (missing subsets of species from the full species pool) in tropical bat surveys for the emerging patterns of species richness (SR) and compositional variation across sites. For 27 bat assemblage data sets from across the tropics, we used correlations between original data sets and subsets with different numbers of species deleted either at random, or according to their rarity in the assemblage, to assess to what extent patterns in SR and composition in data subsets are congruent with those in the initial data set. We then examined to what degree high sample representativeness (r ≥ 0·8) was influenced by biogeographic region, sampling method, sampling effort or structural assemblage characteristics. For SR, correlations between random subsets and original data sets were strong (r ≥ 0·8) with moderate (ca. 20%) species loss. Bias associated with information loss was greater for species composition; on average ca. 90% of species in random subsets had to be retained to adequately capture among-site variation. For nonrandom subsets, removing only the rarest species (on average c. 10% of the full data set) yielded strong correlations (r > 0·95) for both SR and composition. Eliminating greater proportions of rare species resulted in weaker correlations and large variation in the magnitude of observed correlations among data sets. Species subsets that comprised ca. 85% of the original set can be considered reliable surrogates, capable of adequately revealing patterns of SR and temporal or spatial turnover in many tropical bat assemblages. Our analyses thus demonstrate the potential as well as limitations for reducing survey effort and streamlining

  11. Study on the species composition and ecology of anophelines in Addis Zemen, South Gondar, Ethiopia.

    PubMed

    Kindu, Mizan; Aklilu, Esayas; Balkew, Meshesha; Gebre-Michael, Teshome

    2018-03-27

    Malaria is a public health problem in Ethiopia and its transmission is generally unstable and seasonal. For the selection of the most appropriate vector control measures, knowledge on the ecology of the vector is necessary at a local level. Therefore, the objectives of this study were to document the species composition, breeding habitat characteristics and occurrence of anopheline larva in Sheni stream and the vectorial role of the prevailing Anopheles in relation to malaria transmission in Addis Zemen, Ethiopia. Immature anophelines were sampled from breeding habitats and characteristics, such as water temperature, turbidity, water current, water pH and other variables, of the habitats were measured from October 2011 to February 2012. Adult anophelines were sampled inside human dwellings using space spray and Center for Disease Control light traps. Artificial pit shelters and clay pots were also used for outdoor adult collections. Anophelines collected were identified using morphological key. The enzyme-linked immunosorbent assay was applied to detect circumsporozoite proteins of Plasmodium and source of blood meals. A total of 6258 Anopheles larvae were collected and identified morphologically. Five anopheline species were found: An. gambiae (s.l.), An. cinereus, An. demeilloni, An. christi and An. pretoriensis. Anopheles gambiae (s.l.) existed in most of the habitats investigated. Only the former three species were captured in the adult collections. Sun-lit Sheni stream, rain pools, hoof prints, drainage and irrigation canals were found to be habitats of larvae. Anopheles gambiae (s.l.) larvae were most abundantly sampled from sand mining and natural sand pools of Sheni stream. Multiple regression analysis showed that clear, permanent and temporary habitats devoid of mats of algae were the best predictors of An. gambiae (s.l.) larval abundance. It is also the responsible malaria vector in the study area and exhibits anthropophilic and endophagic behaviour. The

  12. Aerobiological Stabilities of Different Species of Gram-Negative Bacteria, Including Well-Known Biothreat Simulants, in Single-Cell Particles and Cell Clusters of Different Compositions

    PubMed Central

    Skogan, Gunnar

    2017-01-01

    ABSTRACT The ability to perform controlled experiments with bioaerosols is a fundamental enabler of many bioaerosol research disciplines. A practical alternative to using hazardous biothreat agents, e.g., for detection equipment development and testing, involves using appropriate model organisms (simulants). Several species of Gram-negative bacteria have been used or proposed as biothreat simulants. However, the appropriateness of different bacterial genera, species, and strains as simulants is still debated. Here, we report aerobiological stability characteristics of four species of Gram-negative bacteria (Pantoea agglomerans, Serratia marcescens, Escherichia coli, and Xanthomonas arboricola) in single-cell particles and cell clusters produced using four spray liquids (H2O, phosphate-buffered saline[PBS], spent culture medium[SCM], and a SCM-PBS mixture). E. coli showed higher stability in cell clusters from all spray liquids than the other species, but it showed similar or lower stability in single-cell particles. The overall stability was higher in cell clusters than in single-cell particles. The highest overall stability was observed for bioaerosols produced using SCM-containing spray liquids. A key finding was the observation that stability differences caused by particle size or compositional changes frequently followed species-specific patterns. The results highlight how even moderate changes to one experimental parameter, e.g., bacterial species, spray liquid, or particle size, can strongly affect the aerobiological stability of Gram-negative bacteria. Taken together, the results highlight the importance of careful and informed selection of Gram-negative bacterial biothreat simulants and also the accompanying particle size and composition. The outcome of this work contributes to improved selection of simulants, spray liquids, and particle size for use in bioaerosol research. IMPORTANCE The outcome of this work contributes to improved selection of simulants

  13. Properties of dissolved and total organic matter in throughfall, stemflow and forest floor leachate of Central European forests

    NASA Astrophysics Data System (ADS)

    Bischoff, S.; Schwarz, M. T.; Siemens, J.; Thieme, L.; Wilcke, W.; Michalzik, B.

    2014-10-01

    For the first time, we investigated the composition of dissolved organic matter (DOM) compared to total OM (TOM, consisting of DOM and particulate OM, POM) in throughfall, stemflow and forest floor leachate of beech and spruce forests using solid state 13C nuclear magnetic resonance spectroscopy. We hypothesized that the composition and properties of OM in forest ecosystem water samples differed between DOM and TOM and between the two tree species. Under beech, a contribution of phyllosphere-derived fresh POM was echoed in structural differences. Compared with DOM, TOM exhibited higher relative intensities for the alkyl C region, representing aliphatic C from less decomposed organic material, and lower relative intensities for lignin-derived and aromatic C of the aryl C region, resulting in lower aromaticity indices and reduced humification intensities. Since differences in the structural composition of DOM and TOM were less pronounced under spruce than under beech, we suspect a~tree species-related effect on the origin of OM composition and resulting properties (e.g. recalcitrance, allelopathic potential).

  14. Larvae of uncommon caridean decapods in the German Bight: Species composition, distribution and abundance

    NASA Astrophysics Data System (ADS)

    Wehrtmann, I. S.; Greve, W.

    1995-03-01

    Typically, the most abundant group of shrimp larvae in the German Bight is formed by representatives of the family Crangonidae. Larvae of the remaining species have been largely ignored, and only scarce information concerning their ecology is available. Thus, the purpose of the present study was to determine the species composition, distribution and abundance of noncrangonid shrimp larvae in the German Bight in July 1990, after the mildest winter of the century. The material is based upon plankton samples collected at 77 stations, covering the entire German Bight. Eight species were identified, as well as larvae of Palaemonidae and Processa-juveniles. Processa nouveli holthuisi (53.0%) and P. modica (31.3%) were predominant in the collection. The distribution of the two species was clearly separated: the main concentration of P. nouveli holthuisi (peak concentration of 1.94 larvae per m3) was confined to the northwest corner of the German Bight, while a majority of P. modica larvae (peak concentration of 0.54 larvae per m3) occurred at the southwesterly stations. The spatial distribution of Caridion steveni and Eualus occultus around Helgoland indicates the presence of an adult population at the only rocky island in the study area. Other taxa, such as larvae of Palaemonidae and juvenile Pandalina brevirostris were collected exclusively in estuarine habitats. Based upon both the results of the present study and comparable data, we conclude that developmental stages of ten non-crangonid species, as well as representatives of Palaemonidae, can be expected to occur in the plankton of the German Bight. The extremely mild temperatures of the preceding winter may have been, in part, responsible for the relatively high densities of some taxa encountered during our plankton survey. We assume that warm winter temperatures favour the immigration, reproduction and survival of cold-sensitive species.

  15. [Fatty acid composition of edible marine fish in Zhoushan, Zhejiang province].

    PubMed

    Gao, Yi-xiong; Yue, Bing; Yu, Xin-wei; He, Jia-lu; Shang, Xiao-hong; Li, Xiao-wei; Wu, Yong-ning

    2013-06-01

    To analyze the main fatty acids in edible marine fish from Zhoushan, Zhejiang province. From September to October 2011, a total of 186 edible marine fish (31 species,6 individual fishes/species) were collected in local markets. Total lipids of edible part were extracted by Folch's method and fatty acids were separated and quantified by gas chromatographic after the homogenization of edible part. The differences of composition of n-6 polyunsaturated fatty acid (n-6 PUFA), n-3 polyunsaturated fatty acid (n-3 PUFA),saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) among fishes were analyzed. Among the 31 fishes, total lipids were highest in Auxis thazard ((13.2 ± 1.2)g/100 g edible part) and lowest in Thamnaconus modestus ((0.6 ± 0.1)g/100 g edible part). Total n-6 PUFA were highest in Mugil cephalus ((875.7 ± 506.4)mg/100 g edible part) and lowest in Seriola quinqueradiata((2.1 ± 1.9)mg/100 g edible part). Total n-3 PUFA were highest in Auxis thazard ((2623.8 ± 426.1)mg/100 g edible part) and lowest in Scoliodon sorrakowah ((82.0 ± 13.9)mg/100 g edible part). SFA were highest in Trachinotus ovatus((3014.9 ± 379.0)mg/100 g edible part) and lowest in Seriola quinqueradiata ((89.7 ± 5.8)mg/100 g edible part). MUFA were highest in Coilia nasus ((3335.7 ± 383.5)mg/100 g edible part) and lowest in Thamnaconus modestus ((32.1 ± 16.9)mg/100 g edible part). There were significant differences of composition of total lipids and of fatty acids among 31 edible marine fish species from Zhoushan.

  16. Relative Pigment Composition and Remote Sensing Reflectance of Caribbean Shallow-Water Corals

    PubMed Central

    Torres-Pérez, Juan L.; Guild, Liane S.; Armstrong, Roy A.; Corredor, Jorge; Zuluaga-Montero, Anabella; Polanco, Ramón

    2015-01-01

    Reef corals typically contain a number of pigments, mostly due to their symbiotic relationship with photosynthetic dinoflagellates. These pigments usually vary in presence and concentration and influence the spectral characteristics of corals. We studied the variations in pigment composition among seven Caribbean shallow-water Scleractinian corals by means of High Performance Liquid Chromatography (HPLC) analysis to further resolve the discrimination of corals. We found a total of 27 different pigments among the coral species, including some alteration products of the main pigments. Additionally, pigments typically found in endolithic algae were also identified. A Principal Components Analysis and a Hierarchical Cluster Analysis showed the separation of coral species based on pigment composition. All the corals were collected under the same physical environmental conditions. This suggests that pigment in the coral’s symbionts might be more genetically-determined than influenced by prevailing physical conditions of the reef. We further investigated the use of remote sensing reflectance (Rrs) as a tool for estimating the total pigment concentration of reef corals. Depending on the coral species, the Rrs and the total symbiont pigment concentration per coral tissue area correlation showed 79.5–98.5% confidence levels demonstrating its use as a non-invasive robust technique to estimate pigment concentration in studies of coral reef biodiversity and health. PMID:26619210

  17. Composition of human faecal microbiota in resistance to Campylobacter infection.

    PubMed

    Kampmann, C; Dicksved, J; Engstrand, L; Rautelin, H

    2016-01-01

    In mice, specific species composition of gut microbiota enhances susceptibility to Campylobacter jejuni but little is known about the specific composition of the human gut microbiota in providing protection from infections caused by enteropathogens. Healthy adult individuals, who travelled in groups from Sweden to destinations with an estimated high risk for acquisition of Campylobacter infection, were enrolled. Faecal samples, collected before travelling and after returning home, were cultured for bacterial enteropathogens, and analysed for Campylobacter by PCR and for the species composition of the microbiota by 16S amplicon massive parallel sequencing. The microbiota compositions were compared between persons who became infected during their travel and those who did not. A total of 63 participants completed the study; 14 became infected with Campylobacter, two with Salmonella and 47 remained negative for the enteropathogens tested. After exclusion of samples taken after antimicrobial treatment, 49 individuals were included in the final analyses. Intra-individual stability of the microbiota was demonstrated for samples taken before travelling. The original diversity of the faecal microbiota was significantly lower among individuals who later became infected compared with those who remained uninfected. The relative abundances of bacteria belonging to the family Lachnospiraceae, and more specifically its two genera Dorea and Coprococcus, were significantly higher among those who remained uninfected. The travel-related infection did not significantly modify the faecal microbiota composition. Species composition of human gut microbiota is important for colonization resistance to Campylobacter infection. Especially individuals with a lower diversity are more susceptible to Campylobacter infection. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  18. Diversity is maintained by seasonal variation in species abundance

    PubMed Central

    2013-01-01

    Background Some of the most marked temporal fluctuations in species abundances are linked to seasons. In theory, multispecies assemblages can persist if species use shared resources at different times, thereby minimizing interspecific competition. However, there is scant empirical evidence supporting these predictions and, to the best of our knowledge, seasonal variation has never been explored in the context of fluctuation-mediated coexistence. Results Using an exceptionally well-documented estuarine fish assemblage, sampled monthly for over 30 years, we show that temporal shifts in species abundances underpin species coexistence. Species fall into distinct seasonal groups, within which spatial resource use is more heterogeneous than would be expected by chance at those times when competition for food is most intense. We also detect seasonal variation in the richness and evenness of the community, again linked to shifts in resource availability. Conclusions These results reveal that spatiotemporal shifts in community composition minimize competitive interactions and help stabilize total abundance. PMID:24007204

  19. Integrating effects of species composition and soil properties to predict shifts in montane forest carbon-water relations.

    PubMed

    Maxwell, Toby M; Silva, Lucas C R; Horwath, William R

    2018-05-01

    This study was designed to address a major source of uncertainty pertaining to coupled carbon-water cycles in montane forest ecosystems. The Sierra Nevada of California was used as a model system to investigate connections between the physiological performance of trees and landscape patterns of forest carbon and water use. The intrinsic water-use efficiency (iWUE)-an index of CO 2 fixed per unit of potential water lost via transpiration-of nine dominant species was determined in replicated transects along an ∼1,500-m elevation gradient, spanning a broad range of climatic conditions and soils derived from three different parent materials. Stable isotope ratios of carbon and oxygen measured at the leaf level were combined with field-based and remotely sensed metrics of stand productivity, revealing that variation in iWUE depends primarily on leaf traits (∼24% of the variability), followed by stand productivity (∼16% of the variability), climatic regime (∼13% of the variability), and soil development (∼12% of the variability). Significant interactions between species composition and soil properties proved useful to predict changes in forest carbon-water relations. On the basis of observed shifts in tree species composition, ongoing since the 1950s and intensified in recent years, an increase in water loss through transpiration (ranging from 10 to 60% depending on parent material) is now expected in mixed conifer forests throughout the region. Copyright © 2018 the Author(s). Published by PNAS.

  20. Feed Conversion, Survival and Development, and Composition of Four Insect Species on Diets Composed of Food By-Products.

    PubMed

    Oonincx, Dennis G A B; van Broekhoven, Sarah; van Huis, Arnold; van Loon, Joop J A

    2015-01-01

    A large part of the environmental impact of animal production systems is due to the production of feed. Insects are suggested to efficiently convert feed to body mass and might therefore form a more sustainable food and/or feed source. Four diets were composed from by-products of food manufacturing and formulated such as to vary in protein and fat content. These were offered to newly hatched Argentinean cockroaches, black soldier flies, yellow mealworms, and house crickets. The first two species are potentially interesting as a feed ingredient, while the latter two are considered edible for humans. Feed conversion efficiency, survival, development time, as well as chemical composition (nitrogen, phosphorus, and fatty acids), were determined. The Argentinean cockroaches and the black soldier flies converted feed more efficiently than yellow mealworms, and house crickets. The first two were also more efficient than conventional production animals. On three of the four diets yellow mealworms and house crickets had a feed conversion efficiency similar to pigs. Furthermore, on the most suitable diet, they converted their feed as efficiently as poultry, when corrected for edible portion. All four species had a higher nitrogen-efficiency than conventional production animals, when corrected for edible portion. Offering carrots to yellow mealworms increased dry matter- and nitrogen-efficiency and decreased development time. Diet affected survival in all species but black soldier flies, and development time was strongly influenced in all four species. The chemical composition of Argentinean cockroaches was highly variable between diets, for black soldier flies it remained similar. The investigated species can be considered efficient production animals when suitable diets are provided. Hence, they could form a sustainable alternative to conventional production animals as a source of feed or food.

  1. Feed Conversion, Survival and Development, and Composition of Four Insect Species on Diets Composed of Food By-Products

    PubMed Central

    Oonincx, Dennis G. A. B.; van Broekhoven, Sarah; van Huis, Arnold; van Loon, Joop J. A.

    2015-01-01

    A large part of the environmental impact of animal production systems is due to the production of feed. Insects are suggested to efficiently convert feed to body mass and might therefore form a more sustainable food and/or feed source. Four diets were composed from by-products of food manufacturing and formulated such as to vary in protein and fat content. These were offered to newly hatched Argentinean cockroaches, black soldier flies, yellow mealworms, and house crickets. The first two species are potentially interesting as a feed ingredient, while the latter two are considered edible for humans. Feed conversion efficiency, survival, development time, as well as chemical composition (nitrogen, phosphorus, and fatty acids), were determined. The Argentinean cockroaches and the black soldier flies converted feed more efficiently than yellow mealworms, and house crickets. The first two were also more efficient than conventional production animals. On three of the four diets yellow mealworms and house crickets had a feed conversion efficiency similar to pigs. Furthermore, on the most suitable diet, they converted their feed as efficiently as poultry, when corrected for edible portion. All four species had a higher nitrogen-efficiency than conventional production animals, when corrected for edible portion. Offering carrots to yellow mealworms increased dry matter- and nitrogen-efficiency and decreased development time. Diet affected survival in all species but black soldier flies, and development time was strongly influenced in all four species. The chemical composition of Argentinean cockroaches was highly variable between diets, for black soldier flies it remained similar. The investigated species can be considered efficient production animals when suitable diets are provided. Hence, they could form a sustainable alternative to conventional production animals as a source of feed or food. PMID:26699129

  2. Development of Selection Criteria and Their Application in Evaluation of CELSS Candidate Species

    NASA Technical Reports Server (NTRS)

    Hoff, J. E.; Howe, J. M.; Mitchell, C. A.

    1982-01-01

    A total of 21 criteria were considered; nine of them fall into the realm of human nutrition and convenience (the "use' criteria), and the remaining 12 are predominantly cultural considerations. Five criteria were considered to be of great importance in the selection of plant species and were given double eight relative to the remaining criteria. "Use' criteria include the following: energy concentration, nutritional composition, palatability, serving size and frequency, processing requirements, use flexibility, toxicity, and human experience. "Cultural' criteria include the following: proportion of edible biomass, yield of edible plant biomass, continuous vs. determinate harvestability, growth habit and morphology, environmental tolerance, photoperiodic and temperature requirements, symbiotic requirements and restrictions, carbon dioxide-light intensity response, suitability for soilless culture, disease resistance, familarity with species, and pollination and propagation. A total of 115 species were evaluated and scored according to suitability for a CELSS.

  3. Compositional variations for alpha-galactosides in different species of leguminosae, brassicaceae, and barley: a chemotaxonomic study based on chemometrics and high-performance capillary electrophoresis.

    PubMed

    Andersen, Keld Ejdrup; Bjergegaard, Charlotte; Møller, Peter; Sørensen, Jens Christian; Sørensen, Hilmer

    2005-07-13

    The contents of raffinose family oligosaccharides (RFO) and sucrose in Brassica, Lupinus, Pisum, and Hordeum species were investigated by chemometric principal component analysis (PCA). Hordeum samples contained sucrose and raffinose, and Brassica samples all contained sucrose, raffinose, and stachyose. In addition to these, the Pisum samples contained verbascose and the Lupinus samples also contained ajugose. High stachyose and low ajugose contents were found in Lupinus albus in contrast to Lupinus angustifolius, having low stachyose and high ajugose contents. Lupinus luteus had average stachyose and ajugose contents, whereas large amounts of verbascose were accumulated in these seeds. Lupinus mutabilis had high stachyose and low ajugose contents, similar to the composition in L. albus but showing higher raffinose content. The Brassica samples also showed compositional RFO variations within the species, and subgroup formations were discovered within the investigated Brassica napus varieties. PCA results indicated compositional variations between the investigated genera and within the various species of value as chemotaxonomic defined parameters and as tools in evaluations of authenticity/falsifications when RFO-containing plants are used as, for example, feed and food additives.

  4. Development of particle induced gamma-ray emission methods for nondestructive determination of isotopic composition of boron and its total concentration in natural and enriched samples.

    PubMed

    Chhillar, Sumit; Acharya, Raghunath; Sodaye, Suparna; Pujari, Pradeep K

    2014-11-18

    We report simple particle induced gamma-ray emission (PIGE) methods using a 4 MeV proton beam for simultaneous and nondestructive determination of the isotopic composition of boron ((10)B/(11)B atom ratio) and total boron concentrations in various solid samples with natural isotopic composition and enriched with (10)B. It involves measurement of prompt gamma-rays at 429, 718, and 2125 keV from (10)B(p,αγ)(7)Be, (10)B(p, p'γ)(10)B, and (11)B(p, p'γ)(11)B reactions, respectively. The isotopic composition of boron in natural and enriched samples was determined by comparing peak area ratios corresponding to (10)B and (11)B of samples to natural boric acid standard. An in situ current normalized PIGE method, using F or Al, was standardized for total B concentration determination. The methods were validated by analyzing stoichiometric boron compounds and applied to samples such as boron carbide, boric acid, carborane, and borosilicate glass. Isotopic compositions of boron in the range of 0.247-2.0 corresponding to (10)B in the range of 19.8-67.0 atom % and total B concentrations in the range of 5-78 wt % were determined. It has been demonstrated that PIGE offers a simple and alternate method for total boron as well as isotopic composition determination in boron based solid samples, including neutron absorbers that are important in nuclear technology.

  5. Chemical and biochemical composition of caviar from different sturgeon species and origins.

    PubMed

    Wirth, M; Kirschbaum, F; Gessner, J; Krüger, A; Patriche, N; Billard, R

    2000-08-01

    The chemical and biochemical composition of caviar in 22 specimens of wild caught and of 2 farmed animals were measured. The results include grain size, protein and fat content, fatty acid composition of triglycerides and phospholipids, as well as the concentrations of relevant heavy metals and chlorinated hydrocarbons. The average protein content varied between 26.2 and 31.1% (wet weight) and fat from 10.9 to 19.4% (wet weight) with lowest values for caviar from farmed sturgeon. The triglycerides and phospholipids contained more n-3 fatty acids, especially eicosapentaenoic and docosahexaenoic acid than n-6 fatty acids. The copper and zinc concentrations varied between 1.20 and 1.69 and 10.3 and 12.4 mg/kg (wet weight), respectively. These values reflect the elevated requirement of sturgeons for these components. Lead content varied between 0.06 and 0.15 mg/kg (wet weight). The cadmium concentrations were less than 5 micrograms/kg (wet weight) leading to the conclusion that no accumulation took place in the eggs. The concentrations of sigma DDT and sigma PCB were extremely high in caviar from Huso huso compared to the samples of the other species thus reflecting the different food habits leading to increased bio-accumulation.

  6. Total Composite Flap Facelift and the Deep-Plane Transition Zone: A Critical Consideration in SMAS-Release Midface Lifting

    PubMed Central

    Mani, Marc

    2016-01-01

    Background Recent anatomic studies suggest the superficial musculoaponeurotic system (SMAS) layer attenuates in the midface. This led the author to switch from a bilamellar high SMAS dissection to a “total composite flap” technique, preserving skin and SMAS/platysma as one layer in a critical “deep-plane transition zone” (DTZ) lateral to the zygomaticus major muscle. This allows traction on the SMAS to translate to the malar fat pad via a “cantilever bridge” effect, which is lost when skin is undermined in the DTZ. Objectives This paper attempts to answer the question of whether the composite flap or bilamellar technique better lifts the midface, comparing groups where the DTZ was undermined: (1) only at a sub-SMAS level; or (2) at both subcutaneous and sub-SMAS levels. Methods Thirty-five patients underwent bilamellar facelifts with skin and SMAS separated in the DTZ. Midfacial elevation was measured using size-matched preoperative and 18-month (average) postoperative photographs for the 70 hemi-midfaces. The same analysis was done for 35 patients undergoing total composite flap facelift, maintaining skin and SMAS as one layer in the DTZ. The two groups were compared. Results In the bilamellar group, the mean percentage of midfacial elevation at 18 months postoperative was 5.5% (range, 0.0%-17.8%). In the composite flap group, the percentage was 11.7% (range, 0.1%-32.3%). The difference was statistically significant. Conclusions Maintaining skin-SMAS attachments in the DTZ improves midface elevation during SMAS facelifting, exploiting a “cantilever bridge” effect of the skin transferring traction on the SMAS to the malar fat pad. Level of Evidence: 4 Therapeutic PMID:26931306

  7. Species composition of mosquito and public perception about Dengue vector of hemorrhagic fever in Bareng Tenes Malang

    NASA Astrophysics Data System (ADS)

    Gama, Zulfaidah Penata; Pratiwi, Jenvia Rista

    2017-11-01

    Dengue Hemorrhagic Fever (DHF) is a mosquito-borne tropical disease caused by the dengue virus. Aedes aegypti and Aedes albopictus are the mosquito vectors of DHF. Malang city was an endemic region of dengue disease in East Java. One of the villages that had a high number of DHF cases was Bareng Tenes. The Case Fatality Rate (CFR) in Malang city totaled 5 patients out of 879 cases (Health Department of Malang city, 2010). Bareng Tenes RW 02 was one of the densely populated regions of Malang city. The objectives of this research were to identify mosquito composition and to analyze the public perception about the DHF vectors in Bareng Tenes RW 02 Malang. This research used two kinds of survey methods of mosquitoes. The first method for collecting larvae was used by direct capture using pipettes from artificial containers and the second method was collecting egg of mosquitoes by using an ovitrap. Public perception was calculated using the questionnaire technique. The accidental sampling technique in this research was Likert scale. The composition of mosquitoes found in Bareng Tenes RW 02 was Aedes aegypti, Aedes albopictus and Culex quinquefasciatus. The mosquito survey showed that Aedes aegypti was the dominant species and the IVI value for the ovitrap survey was 118.06% while the value of IVI for the larval survey was 103.51%. Based on the public perception data, it showed that the community has a very good understanding of DHF knowledge, DHF vectors and ways of DHF prevention, but the undertaken activities by the community have not yet appeared to control the mosquito population especially for their larvae.

  8. Relationships Between Bird Communities and Forest Age, Structure, Species Composition and Fragmentation in the West Gulf Coastal Plain

    Treesearch

    Richard N. Conner; James G. Dickson

    1997-01-01

    Bird communities of the West Gulf Coastal Plain are strongly influenced by the stage of forest succession, species composition of understory and overstory vegetation, and forest structure. Alteration of plant communities through forest management and natural disturbances typically does not eliminate birds as a fauna1 group from the area affected, but will replace some...

  9. Community and ecosystem effects of intraspecific genetic diversity in grassland microcosms of varying species diversity.

    PubMed

    Fridley, Jason D; Grime, J Philip

    2010-08-01

    Studies of whether plant community structure and ecosystem properties depend on the genetic diversity of component populations have been largely restricted to species monocultures and have involved levels of genetic differentiation that do not necessarily correspond to that exhibited by neighboring mature individuals in the field. We established experimental communities of varying intraspecific genetic diversity, using genotypes of eight species propagated from clonal material of individuals derived from a small (100-m2) limestone grassland community, and tested whether genetic diversity (one, four, and eight genotypes per species) influenced community composition and annual aboveground productivity across communities of one, four, and eight species. Eight-species communities were represented by common grass, sedge, and forb species, and four- and one-species communities were represented by four graminoids and the dominant grass Festuca ovina, respectively. After three years of community development, there was a marginal increase of species diversity with increased genetic diversity in four- and eight-species communities, and genetic diversity altered the performance of genotypes in monospecific communities of F. ovina. However, shifts in composition from genetic diversity were not sufficient to alter patterns of community productivity. Neighborhood models describing pairwise interactions between species indicated that genetic diversity decreased the intensity of competition between species in four-species mixtures, thereby promoting competitive equivalency and enhancing species equitability. In F. ovina monocultures, neighborhood models revealed both synergistic and antagonistic interactions between genotypes that were reduced in intensity on more stressful shallow soils. Although the dependence of F. ovina genotype performance on neighborhood genetic composition did not influence total productivity, such dependence was sufficient to uncouple genotype performance

  10. Pyridine-type alkaloid composition affects bacterial community composition of floral nectar

    PubMed Central

    Aizenberg-Gershtein, Yana; Izhaki, Ido; Santhanam, Rakesh; Kumar, Pavan; Baldwin, Ian T.; Halpern, Malka

    2015-01-01

    Pyridine-type alkaloids are most common in Nicotiana species. To study the effect of alkaloid composition on bacterial community composition in floral nectar, we compared the nicotine-rich wild type (WT) N. attenuata, the nicotine biosynthesis-silenced N. attenuata that was rich in anatabine and the anabasine-rich WT N. glauca plants. We found that the composition of these secondary metabolites in the floral nectar drastically affected the bacterial community richness, diversity and composition. Significant differences were found between the bacterial community compositions in the nectar of the three plants with a much greater species richness and diversity in the nectar from the transgenic plant. The highest community composition similarity index was detected between the two wild type plants. The different microbiome composition and diversity, caused by the different pyridine-type alkaloid composition, could modify the nutritional content of the nectar and consequently, may contribute to the change in the nectar consumption and visitation. These may indirectly have an effect on plant fitness. PMID:26122961

  11. Pyridine-type alkaloid composition affects bacterial community composition of floral nectar.

    PubMed

    Aizenberg-Gershtein, Yana; Izhaki, Ido; Santhanam, Rakesh; Kumar, Pavan; Baldwin, Ian T; Halpern, Malka

    2015-06-30

    Pyridine-type alkaloids are most common in Nicotiana species. To study the effect of alkaloid composition on bacterial community composition in floral nectar, we compared the nicotine-rich wild type (WT) N. attenuata, the nicotine biosynthesis-silenced N. attenuata that was rich in anatabine and the anabasine-rich WT N. glauca plants. We found that the composition of these secondary metabolites in the floral nectar drastically affected the bacterial community richness, diversity and composition. Significant differences were found between the bacterial community compositions in the nectar of the three plants with a much greater species richness and diversity in the nectar from the transgenic plant. The highest community composition similarity index was detected between the two wild type plants. The different microbiome composition and diversity, caused by the different pyridine-type alkaloid composition, could modify the nutritional content of the nectar and consequently, may contribute to the change in the nectar consumption and visitation. These may indirectly have an effect on plant fitness.

  12. Nutrient addition shifts plant community composition towards earlier flowering species in some prairie ecoregions in the U.S. Central Plains

    USDA-ARS?s Scientific Manuscript database

    The distribution of flowering across the growing season is governed by each species’ evolutionary history and climatic variability. However, global change factors, such as eutrophication and exotic species invasion, can alter plant community composition and thus change flowering distribution. We ex...

  13. Effects of different tree species on soil organic matter composition, decomposition rates and temperature sensitivities in boreal forest

    NASA Astrophysics Data System (ADS)

    Segura, Javier; Nilsson, Mats B.; Erhagen, Björn; Sparrman, Tobias; Ilstedt, Ulrik; Schleucher, Jürgen; Öquist, Mats

    2017-04-01

    High-latitude ecosystems store a large proportion of the global soil organic matter (SOM) and its mineralization constitutes a major carbon flux to the atmosphere. It has been suggested that different tree species can significantly influence organo-chemical composition of SOM, and rate and temperature sensitivity of SOM decomposition. In this study we used surface soil samples (top 5 cm) from a field experiment where five different tree species (Pinus silvestrys L, Picea abies (L.) H. Karst., Larix decidua Mill., Betula pendula Roth, and Pinus contorta Douglas) were planted on a grass meadow in a randomized block design (n=3) ca. 40 years ago. The samples were incubated at 4, 9, 14, and 19 °C at a soil water potential of -25 kPa (previously determined as optimal water content for decomposition). CO2 production rates were measured hourly for 13 days. CO2 production rates were consequently lowest in the control plots and increased in the order Meadow< Contorta < Betula < Larix < Pinus < Picea. The values ranged between 0.03-0.1, 0.06-0.154, 0.1-0.24 and 0.13-0.36 mg CO2 g-1 OM (dw) h-1 at 4, 9, 14 and 19°C respectively. The temperature response of CO2 production corresponded to Q10s of 2.22 (±0.11), 2.22(±0.15), 2.66 (±0.18), 2.09 (±0.33), 2.38 (±0.31) and 2.31 (±0.09) for meadow, contorta, betula, larix, pinus and picea respectively. Only betula resulted in significantly higher Q10s as compared to the control plots, picea, contorta and larix treatments. These differences in tree species effects on SOM decomposition and its temperature sensitivity will be further discussed in relation to the organo-chemical composition of SOM as determined by pyrolysis gas chromatography-mass spectrometry (Py-GC-MS) and nuclear magnetic resonance spectroscopy (NMR) techniques. We conclude that the temperature response of SOM decomposition rates is likely coupled to tree species composition and may have important implications for soil C dynamics. This finding can have

  14. Shifts in malaria vector species composition and transmission dynamics along the Kenyan coast over the past 20 years.

    PubMed

    Mwangangi, Joseph M; Mbogo, Charles M; Orindi, Benedict O; Muturi, Ephantus J; Midega, Janet T; Nzovu, Joseph; Gatakaa, Hellen; Githure, John; Borgemeister, Christian; Keating, Joseph; Beier, John C

    2013-01-08

    Over the past 20 years, numerous studies have investigated the ecology and behaviour of malaria vectors and Plasmodium falciparum malaria transmission on the coast of Kenya. Substantial progress has been made to control vector populations and reduce high malaria prevalence and severe disease. The goal of this paper was to examine trends over the past 20 years in Anopheles species composition, density, blood-feeding behaviour, and P. falciparum sporozoite transmission along the coast of Kenya. Using data collected from 1990 to 2010, vector density, species composition, blood-feeding patterns, and malaria transmission intensity was examined along the Kenyan coast. Mosquitoes were identified to species, based on morphological characteristics and DNA extracted from Anopheles gambiae for amplification. Using negative binomial generalized estimating equations, mosquito abundance over the period were modelled while adjusting for season. A multiple logistic regression model was used to analyse the sporozoite rates. Results show that in some areas along the Kenyan coast, Anopheles arabiensis and Anopheles merus have replaced An. gambiae sensu stricto (s.s.) and Anopheles funestus as the major mosquito species. Further, there has been a shift from human to animal feeding for both An. gambiae sensu lato (s.l.) (99% to 16%) and An. funestus (100% to 3%), and P. falciparum sporozoite rates have significantly declined over the last 20 years, with the lowest sporozoite rates being observed in 2007 (0.19%) and 2008 (0.34%). There has been, on average, a significant reduction in the abundance of An. gambiae s.l. over the years (IRR = 0.94, 95% CI 0.90-0.98), with the density standing at low levels of an average 0.006 mosquitoes/house in the year 2010. Reductions in the densities of the major malaria vectors and a shift from human to animal feeding have contributed to the decreased burden of malaria along the Kenyan coast. Vector species composition remains heterogeneous but in

  15. Proximate and fatty acid composition of some commercially important fish species from the Sinop region of the Black Sea.

    PubMed

    Kocatepe, Demet; Turan, Hülya

    2012-06-01

    The proximate and fatty acid compositions of the commercially important fish species (Engraulis encrasicolus, Alosa alosa, Belone belone, Scorpaena porcus, Pomatomus saltatrix, Mullus barbatus) from the Sinop region of the Black Sea were examined. The fat contents ranged from 1.26% (for scorpion fish) to 18.12% (for shad). The protein contents were min 14.54% (for red mullet) and maximum 20.26% (for belone). The fatty acid compositions of the fish ranged from 27.83 to 35.91% for saturated fatty acids, 19.50-33.80% for monounsaturated fatty acids and 15.25-40.02% for polyunsaturated fatty acids. Among the saturated fatty acids, palmitic acid (16:0) (17.75-22.20%) was the dominant fatty acid for all the fish species. As a second saturated fatty acid, myristic acid (14:0) was observed in four of the fish species and its content ranged from 4.72 to 7.31%. Whereas, for the other two fish species, the second saturated fatty acid was stearic acid (18:0) ranging between 4.54 and 10.64%. Among the monounsaturated fatty acids, those occurring in the highest proportions were oleic acid (18:1n-9c) (11.67-22.45%) and palmitoleic acid (16:1) (4.50-9.40%). Docosahexaenoic acid (22:6n-3) (5.41-28.52%), eicosapentaenoic acid (20:5n-3) (4.68-11.06) and linoleic acid (18:2n-6) (1.38-3.49%) were dominant polyunsaturated fatty acids, respectively. All the species, in particular the belone, the anchovy and the shad had high levels of the n-3 series.

  16. Species composition, richness and nestedness of lizard assemblages from Restinga habitats along the brazilian coast.

    PubMed

    Rocha, C F D; Vrcibradic, D; Kiefer, M C; Menezes, V A; Fontes, A F; Hatano, F H; Galdino, C A B; Bergallo, H G; Van Sluys, M

    2014-05-01

    Habitat fragmentation is well known to adversely affect species living in the remaining, relatively isolated, habitat patches, especially for those having small range size and low density. This negative effect has been critical in coastal resting habitats. We analysed the lizard composition and richness of restinga habitats in 16 restinga habitats encompassing three Brazilian states (Rio de Janeiro, Espírito Santo and Bahia) and more than 1500km of the Brazilian coast in order to evaluate if the loss of lizard species following habitat reduction occur in a nested pattern or at random, using the "Nestedness Temperature Calculator" to analyse the distribution pattern of lizard species among the restingas studied. We also estimated the potential capacity that each restinga has to maintain lizard species. Eleven lizard species were recorded in the restingas, although not all species occurred in all areas. The restinga with the richest lizard fauna was Guriri (eight species) whereas the restinga with the lowest richness was Praia do Sul (located at Ilha Grande, a large coastal island). Among the restingas analysed, Jurubatiba, Guriri, Maricá and Praia das Neves, were the most hospitable for lizards. The matrix community temperature of the lizard assemblages was 20.49° (= P <0.00001; 5000 randomisations; randomisation temperature = 51.45° ± 7.18° SD), indicating that lizard assemblages in the coastal restingas exhibited a considerable nested structure. The degree in which an area is hospitable for different assemblages could be used to suggest those with greater value of conservation. We concluded that lizard assemblages in coastal restingas occur at a considerable level of ordination in restinga habitats and that some restinga areas such as Jurubatiba, Guriri, Maricá and Praia das Neves are quite important to preserve lizard diversity of restinga environments.

  17. Occurrence of neoxanthin and lutein epoxide cycle in parasitic Cuscuta species.

    PubMed

    Kruk, Jerzy; Szymańska, Renata

    2008-01-01

    In the present study, xanthophyll composition of eight parasitic Cuscuta species under different light conditions was investigated. Neoxanthin was not detected in four of the eight species examined, while in others it occurred at the level of several percent of total xanthophylls. In C. gronovii and C. lupuliformis it was additionally found that the neoxanthin content was considerably stimulated by strong light. In dark-adapted plants, lutein epoxide level amounted to 10-22% of total xanthophylls in only three species, the highest being for C. lupuliformis, while in others it was below 3%, indicating that the lutein epoxide cycle is limited to only certain Cuscuta species. The obtained data also indicate that the presence of the lutein epoxide cycle and of neoxanthin is independent and variable among the Cuscuta species. The xanthophyll cycle carotenoids violaxanthin, antheraxanthin and zeaxanthin were identified in all the examined species and occurred at the level found in other higher plants. The xanthophyll and lutein epoxide cycle pigments showed typical response to high light stress. The obtained results also suggest that the ability of higher plants to synthesize lutein epoxide probably does not depend on the substrate specificity of zeaxanthin epoxidase but on the availability of lutein for the enzyme.

  18. Helminths of three species of opossums (Mammalia, Didelphidae) from Mexico

    PubMed Central

    Acosta-Virgen, Karla; López-Caballero, Jorge; García-Prieto, Luis; Mata-López, Rosario

    2015-01-01

    Abstract From August 2011 to November 2013, 68 opossums (8 Didelphis sp., 40 Didelphis virginiana, 15 Didelphis marsupialis, and 5 Philander opossum) were collected in 18 localities from 12 Mexican states. A total of 12,188 helminths representing 21 taxa were identified (6 trematodes, 2 cestodes, 3 acanthocephalans and 10 nematodes). Sixty-six new locality records, 9 new host records, and one species, the trematode Brachylaima didelphus, is added to the composition of the helminth fauna of the opossums in Mexico. These data, in conjunction with previous records, bring the number of taxa parasitizing the Mexican terrestrial marsupials to 41. Among these species, we recognized a group of helminths typical of didelphids in other parts of the Americas. This group is constituted by the trematode Rhopalias coronatus, the acanthocephalan Oligacanthorhynchus microcephalus and the nematodes Cruzia tentaculata, Gnathostoma turgidum, and Turgida turgida. In general, the helminth fauna of each didelphid species showed a stable taxonomic composition with respect to previously sampled sites. This situation suggests that the rate of accumulation of helminth species in the inventory of these 3 species of terrestrial marsupials in the Neotropical portion of Mexico is decreasing; however, new samplings in the Nearctic portion of this country will probably increase the richness of the helminthological inventory of this group of mammals. PMID:26257556

  19. Temporal Patterns in the Abundance and Species Composition of Spiders on Host Plants of the Invasive Moth Epiphyas postvittana (Lepidoptera: Tortricidae).

    PubMed

    Hogg, Brian N; Mills, Nicholas J; Daane, Kent M

    2017-06-01

    Generalist predators such as spiders may help mitigate the spread and impact of exotic herbivores. The lack of prey specificity and long generation times of spiders may allow them to persist when pests are scarce, and to limit the growth of pest populations before they reach damaging levels. We examined whether resident spiders are likely to play a role in maintaining populations of the invasive light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), below outbreak levels in California. We surveyed the spider community on two E. postvittana host plants, the ornamental Australian tea tree, Leptospermum laevigatum, and the weed French broom, Genista monspessulana, to characterize spider and larval E. postvittana abundance and spider species composition throughout the year. Spider densities and species composition showed slight seasonal changes. Spiders were present during periods of high and low E. postvittana abundance. Anyphaenid hunting spiders, Anyphaena aperta Banks in Australian tea tree and Anyphaena pacifica Banks in French broom, dominated spider species composition at four of five sampled sites, and underwent only slight seasonal variation in abundance. Adult A. aperta were rare at all times of the year, suggesting that high mortality among juvenile A. aperta limits the potential of this species as a predator of E. postvittana. Nevertheless, the continued presence of spiders throughout the year indicates that the resident spider community is likely to play a key role in reducing E. postvittana populations in California. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.

  20. Spatial complexity of carcass location influences vertebrate scavenger efficiency and species composition

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Smith, Joshua B.; Laatsch, Lauren J.; Beasley, James C.

    Scavenging plays an important role in shaping communities through inter- and intra-specific interactions. Although vertebrate scavenger efficiency and species composition is likely influenced by the spatial complexity of environments, heterogeneity in carrion distribution has largely been disregarded in scavenging studies. We tested this hypothesis by experimentally placing juvenile bird carcasses on the ground and in nests in trees to simulate scenarios of nestling bird carrion availability. We used cameras to record scavengers removing carcasses and elapsed time to removal. Carrion placed on the ground was scavenged by a greater diversity of vertebrates and at > 2 times the rate ofmore » arboreal carcasses, suggesting arboreal carrion may represent an important resource to invertebrate scavengers, particularly in landscapes with efficient vertebrate scavenging communities. Nonetheless, six vertebrate species scavenged arboreal carcasses. Rat snakes (Elaphe obsolete), which exclusively scavenged from trees, and turkey vultures (Cathartes aura) were the primary scavengers of arboreal carrion, suggesting such resources are potentially an important pathway of nutrient acquisition for some volant and scansorial vertebrates. Our results highlight the intricacy of carrion-derived food web linkages, and how consideration of spatial complexity in carcass distribution (i.e., arboreal) may reveal important pathways of nutrient acquisition by invertebrate and vertebrate scavenging guilds.« less

  1. Spatial complexity of carcass location influences vertebrate scavenger efficiency and species composition.

    PubMed

    Smith, Joshua B; Laatsch, Lauren J; Beasley, James C

    2017-08-31

    Scavenging plays an important role in shaping communities through inter- and intra-specific interactions. Although vertebrate scavenger efficiency and species composition is likely influenced by the spatial complexity of environments, heterogeneity in carrion distribution has largely been disregarded in scavenging studies. We tested this hypothesis by experimentally placing juvenile bird carcasses on the ground and in nests in trees to simulate scenarios of nestling bird carrion availability. We used cameras to record scavengers removing carcasses and elapsed time to removal. Carrion placed on the ground was scavenged by a greater diversity of vertebrates and at > 2 times the rate of arboreal carcasses, suggesting arboreal carrion may represent an important resource to invertebrate scavengers, particularly in landscapes with efficient vertebrate scavenging communities. Nonetheless, six vertebrate species scavenged arboreal carcasses. Rat snakes (Elaphe obsolete), which exclusively scavenged from trees, and turkey vultures (Cathartes aura) were the primary scavengers of arboreal carrion, suggesting such resources are potentially an important pathway of nutrient acquisition for some volant and scansorial vertebrates. Our results highlight the intricacy of carrion-derived food web linkages, and how consideration of spatial complexity in carcass distribution (i.e., arboreal) may reveal important pathways of nutrient acquisition by invertebrate and vertebrate scavenging guilds.

  2. Spatial complexity of carcass location influences vertebrate scavenger efficiency and species composition

    DOE PAGES

    Smith, Joshua B.; Laatsch, Lauren J.; Beasley, James C.

    2017-08-31

    Scavenging plays an important role in shaping communities through inter- and intra-specific interactions. Although vertebrate scavenger efficiency and species composition is likely influenced by the spatial complexity of environments, heterogeneity in carrion distribution has largely been disregarded in scavenging studies. We tested this hypothesis by experimentally placing juvenile bird carcasses on the ground and in nests in trees to simulate scenarios of nestling bird carrion availability. We used cameras to record scavengers removing carcasses and elapsed time to removal. Carrion placed on the ground was scavenged by a greater diversity of vertebrates and at > 2 times the rate ofmore » arboreal carcasses, suggesting arboreal carrion may represent an important resource to invertebrate scavengers, particularly in landscapes with efficient vertebrate scavenging communities. Nonetheless, six vertebrate species scavenged arboreal carcasses. Rat snakes (Elaphe obsolete), which exclusively scavenged from trees, and turkey vultures (Cathartes aura) were the primary scavengers of arboreal carrion, suggesting such resources are potentially an important pathway of nutrient acquisition for some volant and scansorial vertebrates. Our results highlight the intricacy of carrion-derived food web linkages, and how consideration of spatial complexity in carcass distribution (i.e., arboreal) may reveal important pathways of nutrient acquisition by invertebrate and vertebrate scavenging guilds.« less

  3. Multi-species laser absorption sensors for in situ monitoring of syngas composition

    NASA Astrophysics Data System (ADS)

    Sur, Ritobrata; Sun, Kai; Jeffries, Jay B.; Hanson, Ronald K.

    2014-04-01

    Tunable diode laser absorption spectroscopy sensors for detection of CO, CO2, CH4 and H2O at elevated pressures in mixtures of synthesis gas (syngas: products of coal and/or biomass gasification) were developed and tested. Wavelength modulation spectroscopy (WMS) with 1f-normalized 2f detection was employed. Fiber-coupled DFB diode lasers operating at 2325, 2017, 2290 and 1352 nm were used for simultaneously measuring CO, CO2, CH4 and H2O, respectively. Criteria for the selection of transitions were developed, and transitions were selected to optimize the signal and minimize interference from other species. For quantitative WMS measurements, the collision-broadening coefficients of the selected transitions were determined for collisions with possible syngas components, namely CO, CO2, CH4, H2O, N2 and H2. Sample measurements were performed for each species in gas cells at a temperature of 25 °C up to pressures of 20 atm. To validate the sensor performance, the composition of synthetic syngas was determined by the absorption sensor and compared with the known values. A method of estimating the lower heating value and Wobbe index of the syngas mixture from these measurements was also demonstrated.

  4. Total body composition estimated by standing-posture 8-electrode bioelectrical impedance analysis in male wrestlers.

    PubMed

    Cheng, M-F; Chen, Y-Y; Jang, T-R; Lin, W-L; Chen, J; Hsieh, K-C

    2016-12-01

    Standing-posture 8-electrode bioelectrical impedance analysis is a fast and practical method for evaluating body composition in clinical settings, which can be used to estimate percentage body fat (BF%) and skeletal muscle mass in a subject's total body and body segments. In this study, dual-energy X-ray absorptiometry (DXA) was used as a reference method for validating the standing 8-electrode bioelectrical impedance analysis device BC-418 (BIA 8 , Tanita Corp., Tokyo, Japan). Forty-eight Taiwanese male wrestlers aged from 17.9 to 22.3 years volunteered to participate in this study. The lean soft tissue (LST) and BF% in the total body and body segments were measured in each subject by the BIA 8 and DXA. The correlation coefficients between total body, arm, leg segments impedance index (BI, ht 2 /Z) and lean soft tissue mass measured from DXA were r = 0.902, 0.453, 0.885, respectively (p < 0.01). In addition, the total body and segmental LST estimated by the BIA 8 were highly correlated with the DXA data (r = 0.936, 0.466, 0.886, p < 0.01). The estimation of total body and segmental BF% measured by BIA 8 and DXA also showed a significant correlation (r > 0.820, p < 0.01). The estimated LST and BF% from BIA 8 in the total body and body segments were highly correlated with the DXA results, which indicated that the standing-posture 8-electrode bioelectrical impedance analysis may be used to derive reference measures of LST and BF% in Taiwanese male wrestlers.

  5. Total body composition estimated by standing-posture 8-electrode bioelectrical impedance analysis in male wrestlers

    PubMed Central

    Cheng, M-F; Chen, Y-Y; Jang, T-R; Lin, W-L; Chen, J

    2015-01-01

    Standing-posture 8-electrode bioelectrical impedance analysis is a fast and practical method for evaluating body composition in clinical settings, which can be used to estimate percentage body fat (BF%) and skeletal muscle mass in a subject’s total body and body segments. In this study, dual-energy X-ray absorptiometry (DXA) was used as a reference method for validating the standing 8-electrode bioelectrical impedance analysis device BC-418 (BIA8, Tanita Corp., Tokyo, Japan). Forty-eight Taiwanese male wrestlers aged from 17.9 to 22.3 years volunteered to participate in this study. The lean soft tissue (LST) and BF% in the total body and body segments were measured in each subject by the BIA8 and DXA. The correlation coefficients between total body, arm, leg segments impedance index (BI, ht2/Z) and lean soft tissue mass measured from DXA were r = 0.902, 0.453, 0.885, respectively (p < 0.01). In addition, the total body and segmental LST estimated by the BIA8 were highly correlated with the DXA data (r = 0.936, 0.466, 0.886, p < 0.01). The estimation of total body and segmental BF% measured by BIA8 and DXA also showed a significant correlation (r > 0.820, p < 0.01). The estimated LST and BF% from BIA8 in the total body and body segments were highly correlated with the DXA results, which indicated that the standing-posture 8-electrode bioelectrical impedance analysis may be used to derive reference measures of LST and BF% in Taiwanese male wrestlers. PMID:28090145

  6. Understanding how Seasonality and Shifts in Species Composition Impact Emission Estimates in Semi-Arid Ecosystems

    NASA Astrophysics Data System (ADS)

    Sparks, A.

    2012-12-01

    The importance of wildland fire as a source of trace gas emissions to the atmosphere has been demonstrated in the scientific literature and through numerous NASA funded campaigns to further understand the drivers and impacts of these emissions (e.g., SAFARI 1992, SAFARI 2000, TRACE A, etc). Most studies quantify the emissions using remotely sensed data through multiplying the area burned, the quantity of fuel combusted, and the emission factors of a given gas species (EFX, grams of gas, X, emitted per kilogram of fuel consumed). The latter is known to exhibit considerable uncertainty and indeed a prior study as part of NASA's SAFARI 2000 campaign highlighted a seasonal dependence of carbonaceous gas species emissions. Building off these past studies, the focus of the proposed research is to assess the influence of both seasonality and shifting vegetation composition (via replacement of native with invasive species), on the emissions of trace gases in semi-arid ecosystems. Emissions data will help lower emission factor uncertainties in sagebrush-steppe ecosystems as well as inform management decisions about the best burning times in a season (in terms of air quality and greenhouse gas production).

  7. Genetic origin and composition of a natural hybrid poplar Populus × jrtyschensis from two distantly related species.

    PubMed

    Jiang, Dechun; Feng, Jianju; Dong, Miao; Wu, Guili; Mao, Kangshan; Liu, Jianquan

    2016-04-18

    The factors that contribute to and maintain hybrid zones between distinct species are highly variable, depending on hybrid origins, frequencies and fitness. In this study, we aimed to examine genetic origins, compositions and possible maintenance of Populus × jrtyschensis, an assumed natural hybrid between two distantly related species. This hybrid poplar occurs mainly on the floodplains along the river valleys between the overlapping distributions of the two putative parents. We collected 566 individuals from 45 typical populations of P. × jrtyschensis, P. nigra and P. laurifolia. We genotyped them based on the sequence variations of one maternally inherited chloroplast DNA (cpDNA) fragment and genetic polymorphisms at 20 SSR loci. We further sequenced eight nuclear genes for 168 individuals from 31 populations. Two groups of cpDNA haplotypes characteristic of P. nigra and P. laurifolia respectively were both recovered for P. × jrtyschensis. Genetic structures and coalescent tests of two sets of nuclear population genetic data suggested that P. × jrtyschensis originated from hybridizations between the two assumed parental species. All examined populations of P. × jrtyschensis comprise mainly F1 hybrids from interspecific hybridizations between P. nigra and P. laurifolia. In the habitats of P. × jrtyschensis, there are lower concentrations of soil nitrogen than in the habitats occupied by the other two species. Our extensive examination of the genetic composition of P. × jrtyschensis suggested that it is typical of F1-dominated hybrid zones. This finding plus the low concentration of soil nitrogen in the floodplain soils support the F1-dominated bounded hybrid superiority hypothesis of hybrid zone maintenance for this particular hybrid poplar.

  8. Determination of total arsenic and arsenic species in drinking water, surface water, wastewater, and snow from Wielkopolska, Kujawy-Pomerania, and Lower Silesia provinces, Poland.

    PubMed

    Komorowicz, Izabela; Barałkiewicz, Danuta

    2016-09-01

    Arsenic is a ubiquitous element which may be found in surface water, groundwater, and drinking water. In higher concentrations, this element is considered genotoxic and carcinogenic; thus, its level must be strictly controlled. We investigated the concentration of total arsenic and arsenic species: As(III), As(V), MMA, DMA, and AsB in drinking water, surface water, wastewater, and snow collected from the provinces of Wielkopolska, Kujawy-Pomerania, and Lower Silesia (Poland). The total arsenic was analyzed by inductively coupled plasma mass spectrometry (ICP-MS), and arsenic species were analyzed with use of high-performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC/ICP-MS). Obtained results revealed that maximum total arsenic concentration determined in drinking water samples was equal to 1.01 μg L(-1). The highest concentration of total arsenic in surface water, equal to 3778 μg L(-1) was determined in Trująca Stream situated in the area affected by geogenic arsenic contamination. Total arsenic concentration in wastewater samples was comparable to those determined in drinking water samples. However, significantly higher arsenic concentration, equal to 83.1 ± 5.9 μg L(-1), was found in a snow sample collected in Legnica. As(V) was present in all of the investigated samples, and in most of them, it was the sole species observed. However, in snow sample collected in Legnica, more than 97 % of the determined concentration, amounting to 81 ± 11 μg L(-1), was in the form of As(III), the most toxic arsenic species.

  9. Localized surface plasmon resonance modulation of totally encapsulated VO2/Au/VO2 composite structure

    NASA Astrophysics Data System (ADS)

    Liang, Jiran; Guo, Jinbang; Zhao, Yirui; Zhang, Ying; Su, Tianyu

    2018-07-01

    We design and fabricate a totally encapsulated VO2/Au/VO2 composite structure which is aimed to improve the tunability of the localized surface plasmon resonance (LSPR) peak. In this work, the structure will ensure all the Au NPs’ resonant electric field area is filled with VO2. The modulation range of the totally encapsulated structure is larger than that of the semi-coated structure. To further improve the modulation range, we also explore the VO2 thickness dependence of the structure’s LSPR modulation. With the increase of the top layer VO2 thin film thickness, the modulation range becomes larger. When the thickness is about 80 nm, the absorption peak achieves a largest shift of 112 nm. FDTD solution and equivalent model of series capacitor are used to explain the phenomenon. These results will contribute to the area of metamaterial electromagnetic wave absorber and other fields.

  10. Localized surface plasmon resonance modulation of totally encapsulated VO2/Au/VO2 composite structure.

    PubMed

    Liang, Jiran; Guo, Jinbang; Zhao, Yirui; Zhang, Ying; Su, Tianyu

    2018-07-06

    We design and fabricate a totally encapsulated VO 2 /Au/VO 2 composite structure which is aimed to improve the tunability of the localized surface plasmon resonance (LSPR) peak. In this work, the structure will ensure all the Au NPs' resonant electric field area is filled with VO 2 . The modulation range of the totally encapsulated structure is larger than that of the semi-coated structure. To further improve the modulation range, we also explore the VO 2 thickness dependence of the structure's LSPR modulation. With the increase of the top layer VO 2 thin film thickness, the modulation range becomes larger. When the thickness is about 80 nm, the absorption peak achieves a largest shift of 112 nm. FDTD solution and equivalent model of series capacitor are used to explain the phenomenon. These results will contribute to the area of metamaterial electromagnetic wave absorber and other fields.

  11. Resistance Level of Mosquito Species (Diptera: Culicidae) from Shandong Province, China.

    PubMed

    Liu, Hong-Mei; Yang, Pei-Pei; Cheng, Peng; Wang, Hai-Fang; Liu, Li-Juan; Huang, Xiaodan; Zhao, Yu-Qiang; Wang, Huai-Wei; Zhang, Chong-Xing; Gong, Mao-Qing

    2015-01-01

    This study describes the aquatic habitats, species composition, and the insecticide resistance level of the mosquito Culex pipiens pallens in Shandong Province, China. A cross-sectional survey of mosquito larval habitats was conducted from May to November 2014 to determine the species composition and larval abundance. Larvae were collected using the standard dipping technique, and a total of four habitat types were sampled. The fourth instar larvae of Cx. pipiens pallens collected in each habitat type were tested for resistance to five insecticides according to a WHO bioassay. A total of 7,281 mosquito larvae were collected, of which 399 (5.48%) were categorized as Anopheles mosquito larvae (An. sinensis), 6636 (91.14%) as culicine larvae (Cx. pipiens pallens, Cx. tritaeniorhynchus, Cx. halifaxii, and Cx. bitaeniorhynchus), 213 (2.93%) as Armigeres larvae, and 33 (0.45%) as Aedes larvae (Aedes albopictus). In addition, a total of 1,149 mosquito pupae were collected. Culex larvae were distributed in all habitats investigated. Tukeys HSD analysis showed that roadside drainages were the most productive habitat type for Culex larvae. Armigeres species were found only in drains, Aedes only in water tanks, and Anopheles in water that was comparatively clear and rich in emergent plants. Bioassay showed that the maximum resistance level of Cx. pipiens pallens was to deltamethrin, while it was lowest to plifenate. The productivity of various mosquitoes in different habitat types is very heterogeneous. It is particularly important to modify human activity and the environment to achieve effective mosquito vector control. For effective larval control, the type of habitat should be considered, and the most productive habitat type should be given priority in mosquito abatement programs.

  12. Structure of bird communities in eucalyptus plantations: nestedness as a pattern of species distribution.

    PubMed

    Jacoboski, L I; Mendonça-Lima, A de; Hartz, S M

    2016-04-19

    Replacement of native habitats by tree plantations has increased dramatically in Brazil, resulting in loss of structural components for birds, such as appropriate substrates for foraging and nesting. Tree plantations can also reduce faunal richness and change the composition of bird species. This study evaluated the structure of avian communities in eucalyptus plantations of different ages and in a native forest. We classified species as habitat specialists or generalists, and assessed if the species found in eucalyptus plantations are a subset of the species that occur in the native forest. Forty-one sampling sites were evaluated, with three point counts each, in a native forest and in eucalyptus plantations of four different ages. A total of 71 bird species were identified. Species richness and abundance were higher in the native forest, reflecting the greater heterogeneity of the habitat. The composition of bird species also differed between the native forest and plantations. The species recorded in the plantations represented a subset of the species of the native forest, with a predominance of generalist species. These species are more tolerant of habitat changes and are able to use the plantations. The commercial plantations studied here can serve as a main or occasional habitat for these generalists, especially for those that are semi-dependent on edge and forest. The bird species most affected by silviculture are those that are typical of open grasslands, and those that are highly dependent on well-preserved forests.

  13. Relationships among Egg Size, Composition, and Energy: A Comparative Study of Geminate Sea Urchins

    PubMed Central

    McAlister, Justin S.; Moran, Amy L.

    2012-01-01

    Egg size is one of the fundamental parameters in the life histories of marine organisms. However, few studies have examined the relationships among egg size, composition, and energetic content in a phylogenetically controlled context. We investigated the associations among egg size, composition, and energy using a comparative system, geminate species formed by the closure of the Central American Seaway. We examined western Atlantic (WA) and eastern Pacific (EP) species in three echinoid genera, Echinometra, Eucidaris, and Diadema. In the genus with the largest difference in egg size between geminates (Echinometra), the eggs of WA species were larger, lipid rich and protein poor compared to the smaller eggs of their EP geminate. In addition, the larger WA eggs had significantly greater total egg energy and summed biochemical constituents yet significantly lower egg energy density (energy-per-unit-volume). However, the genera with smaller (Eucidaris) or no (Diadema) differences in egg size were not significantly different in summed biochemical constituents, total egg energy, or energy density. Theoretical models generally assume a strong tradeoff between egg size and fecundity that limits energetic investment and constrains life history evolution. We show that even among closely-related taxa, large eggs cannot be assumed to be scaled-up small eggs either in terms of energy or composition. Although our data comes exclusively from echinoid echinoderms, this pattern may be generalizable to other marine invertebrate taxa. Because egg composition and egg size do not necessarily evolve in lockstep, selective factors such as sperm limitation could act on egg volume without necessarily affecting maternal or larval energetics. PMID:22911821

  14. Acceleration and novelty: community restoration speeds recovery and transforms species composition in Andean cloud forest.

    PubMed

    Wilson, Sarah Jane; Rhemtulla, Jeanine M

    2016-01-01

    Community-based tropical forest restoration projects, often promoted as a win-win solution for local communities and the environment, have increased dramatically in number in the past decade. Many such projects are underway in Andean cloud forests, which, given their extremely high biodiversity and history of extensive clearing, are understudied. This study investigates the efficacy of community-based tree-planting projects to accelerate cloud forest recovery, as compared to unassisted natural regeneration. This study takes place in northwest Andean Ecuador, where the majority of the original, highly diverse cloud forests have been cleared, in five communities that initiated tree-planting projects to restore forests in 2003. In 2011, we identified tree species along transects in planted forests (n = 5), naturally regenerating forests (n = 5), and primary forests (n = 5). We also surveyed 120 households about their restoration methods, tree preferences, and forest uses. We found that tree diversity was higher in planted than in unplanted secondary forest, but both were less diverse than primary forests. Ordination analysis showed that all three forests had distinct species compositions, although planted forests shared more species with primary forests than did unplanted forests. Planted forests also contained more animal-dispersed species in both the planted canopy and in the unplanted, regenerating understory than unplanted forests, and contained the highest proportion of species with use value for local people. While restoring forest increased biodiversity and accelerated forest recovery, restored forests may also represent novel ecosystems that are distinct from the region's previous ecosystems and, given their usefulness to people, are likely to be more common in the future.

  15. Fetal subcutaneous tissue measurements in pregnancy as a predictor of neonatal total body composition.

    PubMed

    O'Connor, Clare; Doolan, Anne; O'Higgins, Amy; Segurado, Ricardo; Sheridan-Pereiraet, Margaret; Turner, Michael J; Stuart, Bernard; Kennelly, Máireád M

    2014-10-01

    The purpose of this study was to examine the relationship between prenatal measures of subcutaneous tissue as surrogate markers of fetal nutritional status and correlate them with neonatal total body composition. This prospective longitudinal study of 62 singleton pregnancies obtained serial biometry and subcutaneous tissue measurements at 28, 33 and 38 weeks gestation. These measurements were then correlated with neonatal body composition, which was analysed using the PEAPOD™ Infant Body Composition System (Cosmed USA, Concord, CA, USA). At 38 weeks gestation, fetal abdominal subcutaneous tissue (FAST) in millimetres was significantly associated with infant fat mass at delivery (+64 g per mm of FAST, p < 0.001). Thigh fat (TF) at 28 weeks gestation was associated with infant fat mass at delivery (+79 g/mm TF, p = 0.023). TF at 38 weeks gestation was associated with infant fat mass (+63/mm TF, p = 0.004). TF and FAST at 38 weeks were also predictive of both birth weight and increased abdominal circumference (AC) (p = 0.001) with FAST measurement predicting an additional 5.7 mm in AC per millimetre of FAST (p = 0.002) and TF predicting an additional 6.9 mm per mm of TF (p = 0.002). We believe that this study further validates the use of prenatal measures of subcutaneous tissue and may help to highlight fetuses at risk of newborn adiposity and metabolic syndrome. © 2014 John Wiley & Sons, Ltd.

  16. Variation in acoustic behavior of delphinids in the Pacific Ocean based on school size and species composition

    NASA Astrophysics Data System (ADS)

    Rankin, Shannon; Barlow, Jay

    2005-04-01

    Variation in acoustic behavior based on school size and species composition was examined for surveys in the eastern tropical Pacific (2000), along the U.S. West Coast (2001), and in the U.S. EEZ surrounding Hawaii (2002). Sounds were monitored using a towed hydrophone array, and vocal schools were defined as those producing any combination of whistles, burst pulses, and/or echolocation clicks. Delphinid schools containing mixed species were consistently more vocal than single species schools. Vocal schools of Stenella attenuata, S. longirostris, Delphinus delphis, and Lissodelphis borealis were significantly larger than non-vocal schools. Vocal schools of Tursiops truncatus and Grampus griseus were somewhat larger than non-vocal schools, although this relationship was not significant. There was no relationship between group size and vocal activity for S. coeruleoalba, Steno bredanensis, and Globicephala spp. For species without a strong group size effect, all but T. truncatus were more vocal in the Hawaiian waters. The ability to use acoustic techniques in dolphin population estimation depends on their effectiveness in consistently detecting dolphin schools. This study suggests that small single-species schools of S. attenuata, S. longirostris, D. delphis, and L. borealis are more likely to be missed during acoustic monitoring in these regions.

  17. Natural Chemical Composition of Commercial Fish Species: Characterisation of Pangasius, Wild and Farmed Turbot and Barramundi

    PubMed Central

    Manthey-Karl, Monika; Lehmann, Ines; Ostermeyer, Ute; Schröder, Ute

    2016-01-01

    To comply with the relevant legal requirements and correct labelling, it is necessary for business operators and inspection authorities to know the natural characteristics of the raw material. This study gives a comprehensive overview of muscle flesh composition of farmed and wild Atlantic turbot (Scophthalmus maximus) and barramundi (Lates calcarifer) and of farmed pangasius (Pangasianodon hypophthalmus). The proximate composition, di- and triphosphates and citric acid values are presented in order to evaluate possible indicators for a hidden treatment during processing to fillets. All moisture contents were ≤80%. Even for pangasius, protein values for deep skinned fillets of ≥18% were determined. Only small quantities of naturally occurring citric acid (up to 0.03 g·kg−1) were detectable. The lipid content was the most varying main component within the different species, ranging between 1.2% to 2.0% and 0.3% to 3.0% for farmed turbot and barramundi, respectively. Pangasius flesh had a mean lipid content of 7.8%. Trimming and separation of the red layer reduced the lipid content of the commercially sold white-flesh fillets to 2.7% to 3.5%. Fatty acids profiles, free amino acids, and minerals were analysed to show the nutritional quality of the aquaculture fish species and compared to wild turbot and barramundi. Despite some natural variation, these components can be considered as comparable. PMID:28231154

  18. Species composition of the genus Saprolegnia in fin fish aquaculture environments, as determined by nucleotide sequence analysis of the nuclear rDNA ITS regions.

    PubMed

    de la Bastide, Paul Y; Leung, Wai Lam; Hintz, William E

    2015-01-01

    The ITS region of the rDNA gene was compared for Saprolegnia spp. in order to improve our understanding of nucleotide sequence variability within and between species of this genus, determine species composition in Canadian fin fish aquaculture facilities, and to assess the utility of ITS sequence variability in genetic marker development. From a collection of more than 400 field isolates, ITS region nucleotide sequences were studied and it was determined that there was sufficient consistent inter-specific variation to support the designation of species identity based on ITS sequence data. This non-subjective approach to species identification does not rely upon transient morphological features. Phylogenetic analyses comparing our ITS sequences and species designations with data from previous studies generally supported the clade scheme of Diéguez-Uribeondo et al. (2007) and found agreement with the molecular taxonomic cluster system of Sandoval-Sierra et al. (2014). Our Canadian ITS sequence collection will thus contribute to the public database and assist the clarification of Saprolegnia spp. taxonomy. The analysis of ITS region sequence variability facilitated genus- and species-level identification of unknown samples from aquaculture facilities and provided useful information on species composition. A unique ITS-RFLP for the identification of S. parasitica was also described. Copyright © 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  19. Abundance of common species, not species richness, drives delivery of a real-world ecosystem service.

    PubMed

    Winfree, Rachael; Fox, Jeremy W; Williams, Neal M; Reilly, James R; Cariveau, Daniel P

    2015-07-01

    Biodiversity-ecosystem functioning experiments have established that species richness and composition are both important determinants of ecosystem function in an experimental context. Determining whether this result holds for real-world ecosystem services has remained elusive, however, largely due to the lack of analytical methods appropriate for large-scale, associational data. Here, we use a novel analytical approach, the Price equation, to partition the contribution to ecosystem services made by species richness, composition and abundance in four large-scale data sets on crop pollination by native bees. We found that abundance fluctuations of dominant species drove ecosystem service delivery, whereas richness changes were relatively unimportant because they primarily involved rare species that contributed little to function. Thus, the mechanism behind our results was the skewed species-abundance distribution. Our finding that a few common species, not species richness, drive ecosystem service delivery could have broad generality given the ubiquity of skewed species-abundance distributions in nature. © 2015 John Wiley & Sons Ltd/CNRS.

  20. Community composition and diversity of ground beetles (Coleoptera: Carabidae) in Yaoluoping National Nature Reserve

    PubMed Central

    Li, Wen-Bo; Liu, Nai-Yi; Wu, Yun-He; Zhang, Yu-Cai; Xu, Qin; Chu, Jun; Wang, Shu-Yan

    2017-01-01

    Abstract This study used pitfall trapping to examine community composition and diversity of ground beetles in five different habitats (coniferous, deciduous, mixed coniferous, farmland, and settlements) within Anhui Yaoluoping National Nature Reserve from May to September 2014. In total, 1,352 ground beetles were collected, belonging to 16 genera and 44 species. Of these, four dominant species Dolichus halensis, Harpalus pastor, Carabus casaleianus, and Pheropsophus jessoensis were identified, respectively, comprising 370, 177, 131, and 123 individuals. The deciduous forest showed greater diversity (3.78 according to Shannon–Weiner index), equitability (0.80 according to Pielou’s index), and dominance (9.52 according to Simpson’s index) when compared with farmland, but species richness in the deciduous forest (27) was lower than that in farmland (35). One-way analysis of variance showed that ground beetle species composition and abundance among different habitats varied significantly. Cluster analysis and principal coordinate analysis showed that farmland shared low community similarity with other habitat types, and coniferous and mixed coniferous forests shared similar community types. Our results indicate that species composition, abundance, and diversity of ground beetles are affected by different habitat types, with deciduous forest types being critical in maintaining the diversity of rare species. We recommend reducing cultivated farmland area and increasing the area of carefully planned deciduous forest in order to better protect ground beetle diversity in the region.

  1. Essential oil composition of Pimpinella cypria and its insecticidal, cytotoxic, and antimicrobial activity

    USDA-ARS?s Scientific Manuscript database

    A water-distilled essential oil from the aerial parts of Pimpinella cypria Boiss. (Apiaceae), an endemic species in northern Cyprus, was analyzed by GC and GC-MS. Forty-five compounds were identified in the oil and these comprised 81.7% of the total composition. The compound classes in the oil were ...

  2. Inter-habitat variation in density and size composition of reef fishes from the Cuban Northwestern shelf.

    PubMed

    Aguilar, Consuelo; González-Sansón, Gaspar; Cabrera, Yureidy; Ruiz, Alexei; Curry, R Allen

    2014-06-01

    Movement and exchange of individuals among habitats is critical for the dynamics and success of reef fish populations. Size segregation among habitats could be taken as evidence for habitat connectivity, and this would be a first step to formulate hypotheses about ontogenetic inter-habitat migrations. The primary goal of our research was to find evidence of inter-habitat differences in size distributions and density of reef fish species that can be classified a priori as habitat-shifters in an extensive (-600km2) Caribbean shelf area in NW Cuba. We sampled the fish assemblage of selected species using visual census (stationary and transect methods) in 20 stations (sites) located in mangrove roots, patch reefs, inner zone of the crest and fore reef (12-16m depth). In each site, we performed ten censuses for every habitat type in June and September 2009. A total of 11 507 individuals of 34 species were counted in a total of 400 censuses. We found significant differences in densities and size compositions among reef and mangrove habitats, supporting the species-specific use of coastal habitats. Adults were found in all habitats. Reef habitats, mainly patch reefs, seem to be most important for juvenile fish of most species. Mangroves were especially important for two species of snappers (Lutjanus apodus and L. griseus), providing habitat for juveniles. These species also displayed well defined gradients in length composition across the shelf.

  3. Tree specie effects on soil microbial community composition and greenhouse gases emissions in a Mediterranean ecotone forest

    NASA Astrophysics Data System (ADS)

    Fernandez, Maria Jose; Ortiz, Carlos; Kitzler, Barbara; Curiel, Jorge; Rubio, Agustin

    2016-04-01

    Over recent decades in the Iberian Peninsula, altitudinal shifts from Pinus sylvestris L. to Quercus pyrenaica Willd species has been observed as a consequence of Global Change, meaning changes in temperature, precipitation, land use and forestry. The forest conversion from pine to oak can alter the litter quality and quantity provided to the soil and thereby the soil microbial community composition and functioning. Since soil microbiota plays an important role in organic matter decomposition, and this in turn is key in biogeochemical cycles and forest ecosystems productivity, the rate in which forests produce and consume greenhouse gases can be also affected by changes in forest composition. In other words, changes in litter decomposition will ultimately affect downstream carbon and nitrogen dynamics although this impact is uncertain. In order to predict changes in carbon and nitrogen stocks in Global Change scenarios, it is necessary to deepen the impact of vegetation changes on soil microbial communities, litter decomposition dynamics (priming effect) and the underlying interactions between these factors. To test this, we conducted a full-factorial transplant microcosms experiment mixing both fresh soils and litter from Pyrenean oak, Scots pine and mixed stands collected inside their transitional area in Central Spain. The microcosms consisted in soil cylinders inside Kilner jars used as chambers inside an incubator. In this experiment, we investigated how and to what extent the addition of litter with different quality (needles, oak leaves and mixed needles-leaves) to soil inoculums with contrasting soil microbiota impact on (i) soil CO2, NO, N2O and CH4 efflux rates, (ii) total organic carbon and nitrogen and (iii) dissolved organic carbon and nitrogen. Furthermore, we assessed if these responses were controlled by changes in the microbial community structure using the PLFA analyses prior and after the incubation period of 54 days.

  4. Silvicultural options for early-successional stands in Maine: 6-year results of the Silvicultural Intensity and Species Composition Experiment

    Treesearch

    Andrew S. Nelson; Robert G. Wagner; Mike R. Saunders

    2014-01-01

    The Silvicultural Intensity and Species Composition (SIComp) experiment was installed in 2003 on a recently clearcut mixedwood site within the Penobscot Experimental Forest in east-central Maine. This study was initiated because the response of early-successional stands to various intensities of silviculture was poorly understood in the region. The goal of SIComp is to...

  5. Evaluation of NOy Species Composition and PM2.5 Mass Balance Closure during SOAS at Centreville, AL

    NASA Astrophysics Data System (ADS)

    Baumann, K.; Edgerton, E.; Jansen, J. J.; Shaw, S. L.; Knipping, E. M.

    2013-12-01

    The SEARCH network site near Centreville, AL served as the comprehensive scientific ground measurement site during the June 1 to July 15, 2013 Southern Oxidant and Aerosol Study (SOAS). Besides basic meteorological parameters, the suite of aerosol parameters routinely measured in SEARCH includes individual reactive nitrogen species (NO, NO2, HNO3, alkyl-nitrates, per-nitrates, particulate nitrate) and PM2.5 species sulfate, nitrate and ammonium ions, organic and elemental carbon (OC and EC). Ambient measurements of these species made during the 6-week SOAS campaign are being evaluated and compared with their respective total NOy and PM2.5 mass, measured via reduction on 350°C Mo to NO with subsequent CLD and TEOM, respectively. Thanks to this separate and independent determination of total NOy and PM2.5 mass, the level of closure with the sum of individual species concentrations serves as independent quality assurance and plausibility check. More importantly, though, the lack of closure allows investigation into the reasons why. Especially in the case of PM2.5, the lack of mass closure is predominantly caused by other organic components, whose mass remains unknown due to the specific OC measurement. In order to report organic mass (OM) the factor OM/OC is commonly used and can vary between 1.4 and 2.5 (e.g. Turpin and Lim, 2001; Aiken et al., 2008), largely due to the oxygen content in OM. Measurements made during SOAS indicate that the OM/OC factor can vary significantly from hour to hour depending on time of day and air mass transported to the site. Dependencies for varying OM/OC in PM2.5 as well as for varying lack of NOy closure are investigated and presented. Overall, NO and particulate nitrate were always the smallest contributors to NOy, both showing highest values early mornings before and around sunrise. Organic and per-nitrates showed broad peaks late mornings, while HNO3 peaked mid to late afternoon. Nighttime levels of NO2 were systematically higher

  6. Effect of somatic cell count and mastitis pathogens on milk composition in Gyr cows

    PubMed Central

    2013-01-01

    Background Gyr cows are well adapted to tropical conditions, resistant to some tropical diseases and have satisfactory milk production. However, Gyr dairy herds have a high prevalence of subclinical mastitis, which negatively affects their milk yield and composition. The objectives of this study were (i) to evaluate the effects of seasonality, mammary quarter location (rear x front), mastitis-causing pathogen species, and somatic cell count (SCC) on milk composition in Gyr cows with mammary quarters as the experimental units and (ii) to evaluate the effects of seasonality and somatic cell count (SCC) on milk composition in Gyr cows with cows as the experimental units. A total of 221 lactating Gyr cows from three commercial dairy farms were selected for this study. Individual foremilk quarter samples and composite milk samples were collected once a month over one year from all lactating cows for analysis of SCC, milk composition, and bacteriological culture. Results Subclinical mastitis reduced lactose, nonfat solids and total solids content, but no difference was found in the protein and fat content between infected and uninfected quarters. Seasonality influenced milk composition both in mammary quarters and composite milk samples. Nevertheless, there was no effect of mammary quarter position on milk composition. Mastitis-causing pathogens affected protein, lactose, nonfat solids, and total solids content, but not milk fat content. Somatic cell count levels affected milk composition in both mammary quarters and composite samples of milk. Conclusions Intramammary infections in Gyr cows alter milk composition; however, the degree of change depends on the mastitis-causing pathogen. Somatic cell count is negatively associated with reduced lactose and nonfat solids content in milk. Seasonality significantly affects milk composition, in which the concentration of lactose, fat, protein, nonfat solids and total solids differs between dry and wet seasons in Gyr cows. PMID

  7. Seasonal and interannual variability in the taxonomic composition and production dynamics of phytoplankton assemblages in Crater Lake, Oregon

    USGS Publications Warehouse

    C. David, McIntire; Larson, Gary L.; Truitt, Robert E.

    2007-01-01

    Taxonomic composition and production dynamics of phytoplankton assemblages in Crater Lake, Oregon, were examined during time periods between 1984 and 2000. The objectives of the study were (1) to investigate spatial and temporal patterns in species composition, chlorophyll concentration, and primary productivity relative to seasonal patterns of water circulation; (2) to explore relationships between water column chemistry and the taxonomic composition of the phytoplankton; and (3) to determine effects of primary and secondary consumers on the phytoplankton assemblage. An analysis of 690 samples obtained on 50 sampling dates from 14 depths in the water column found a total of 163 phytoplankton taxa, 134 of which were identified to genus and 101 were identified to the species or variety level of classification. Dominant species by density or biovolume included Nitzschia gracilis, Stephanodiscus hantzschii, Ankistrodesmus spiralis, Mougeotia parvula, Dinobryon sertularia, Tribonema affine, Aphanocapsa delicatissima, Synechocystis sp., Gymnodinium inversum, and Peridinium inconspicuum. When the lake was thermally stratified in late summer, some of these species exhibited a stratified vertical distribution in the water column. A cluster analysis of these data also revealed a vertical stratification of the flora from the middle of the summer through the early fall. Multivariate test statistics indicated that there was a significant relationship between the species composition of the phytoplankton and a corresponding set of chemical variables measured for samples from the water column. In this case, concentrations of total phosphorus, ammonia, total Kjeldahl nitrogen, and alkalinity were associated with interannual changes in the flora; whereas pH and concentrations of dissolved oxygen, orthophosphate, nitrate, and silicon were more closely related to spatial variation and thermal stratification. The maximum chlorophyll concentration when the lake was thermally stratified

  8. Species composition, richness, and distribution of marine bivalve molluscs in Bahía de Mazatlán, México

    PubMed Central

    Esqueda-González, María del Carmen; Ríos-Jara, Eduardo; Galván-Villa, Cristian Moises; Rodríguez-Zaragoza, Fabian Alejandro

    2014-01-01

    Abstract We describe the composition and distribution of bivalve molluscs from the sandy and rocky intertidal and the shallow subtidal environments of Bahía de Mazatlán, México. The bivalve fauna of the bay is represented by 89 living species in 28 families, including 37 new records and four range extensions: Lithophaga hastasia, Adula soleniformis, Mactrellona subalata, and Strigilla ervilia. The number of species increases from the upper (44) and lower intertidal (53) to the shallow subtidal (76), but only 11 (17%) have a wide distribution in the bay (i.e., found in all sampling sites and environments). The bivalve assemblages are composed of four main life forms: 27 epifaunal species, 26 infaunal, 16 semi-infaunal, and 20 endolithic. A taxonomic distinctness analysis identified the sampling sites and environments that contribute the most to the taxonomic diversity (species to suborder categories) of the bay. The present work increased significantly (31%) to 132 species previous inventories of bivalves of Bahía de Mazatlán. These species represent 34% of the bivalve diversity of the southern Golfo de California and approximately 15% of the Eastern Tropical Pacific region. PMID:24843252

  9. Essential oil composition and antimicrobial activity of wild and cultivated Moroccan Achillea ageratum L.: a rare and threatened medicinal species.

    PubMed

    El Bouzidi, Laila; Abbad, Abdelaziz; Hassani, Lahcen; Fattarsi, Karine; Leach, David; Markouk, Mohammed; Legendre, Laurent; Bekkouche, Khalid

    2012-03-01

    The essential oils of leaves and flowers of the wild and cultivated Moroccan Achillea ageratum L., a rare and threatened medicinal species, were examined by GC/MS, and their chemical compositions were compared. At least nine components were identified in both wild and cultivated A. ageratum oils, representing more than 95% of the oils. Artemisyl acetate (62.34-78.79%), yomogi alcohol (4.89-12.40%), santolina alcohol (4.86-11.77%), and artemisia alcohol (3.36-7.04%) were the major compounds. Terpene-alcohol proportion was higher in wild A. ageratum than in cultivated A. ageratum. The antibacterial analysis showed that both oils presented high activity against all the studied Gram-positive strains in a range of MIC values from 2.55 to 7.02 mg/ml, but they appeared not effective against the tested Gram-negative ones (MIC values 20.40-41.10 mg/ml). They also exhibited remarkable antifungal activities against Candida species with MIC values ranging from 5.83 to 8.42 mg/ml. From these results, it was concluded that domestication of this threatened medicinal species using clonal propagation did not significantly affect its chemical composition and consequently its antimicrobial properties. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich.

  10. Extracellular DNA in single- and multiple-species unsaturated biofilms.

    PubMed

    Steinberger, R E; Holden, P A

    2005-09-01

    The extracellular polymeric substances (EPS) of bacterial biofilms form a hydrated barrier between cells and their external environment. Better characterization of EPS could be useful in understanding biofilm physiology. The EPS are chemically complex, changing with both bacterial strain and culture conditions. Previously, we reported that Pseudomonas aeruginosa unsaturated biofilm EPS contains large amounts of extracellular DNA (eDNA) (R. E. Steinberger, A. R. Allen, H. G. Hansma, and P. A. Holden, Microb. Ecol. 43:416-423, 2002). Here, we investigated the compositional similarity of eDNA to cellular DNA, the relative quantity of eDNA, and the terminal restriction fragment length polymorphism (TRFLP) community profile of eDNA in multiple-species biofilms. By randomly amplified polymorphic DNA analysis, cellular DNA and eDNA appear identical for P. aeruginosa biofilms. Significantly more eDNA was produced in P. aeruginosa and Pseudomonas putida biofilms than in Rhodococcus erythropolis or Variovorax paradoxus biofilms. While the amount of eDNA in dual-species biofilms was of the same order of magnitude as that of of single-species biofilms, the amounts were not predictable from single-strain measurements. By the Shannon diversity index and principle components analysis of TRFLP profiles generated from 16S rRNA genes, eDNA of four-species biofilms differed significantly from either cellular or total DNA of the same biofilm. However, total DNA- and cellular DNA-based TRFLP analyses of this biofilm community yielded identical results. We conclude that extracellular DNA production in unsaturated biofilms is species dependent and that the phylogenetic information contained in this DNA pool is quantifiable and distinct from either total or cellular DNA.

  11. Arbuscular mycorrhizal fungi alter above- and below-ground chemical defense expression differentially among Asclepias species.

    PubMed

    Vannette, Rachel L; Hunter, Mark D; Rasmann, Sergio

    2013-01-01

    Below-ground (BG) symbionts of plants can have substantial influence on plant growth and nutrition. Recent work demonstrates that mycorrhizal fungi can affect plant resistance to herbivory and the performance of above- (AG) and BG herbivores. Although these examples emerge from diverse systems, it is unclear if plant species that express similar defensive traits respond similarly to fungal colonization, but comparative work may inform this question. To examine the effects of arbuscular mycorrhizal fungi (AMF) on the expression of chemical resistance, we inoculated 8 species of Asclepias (milkweed)-which all produce toxic cardenolides-with a community of AMF. We quantified plant biomass, foliar and root cardenolide concentration and composition, and assessed evidence for a growth-defense tradeoff in the presence and absence of AMF. As expected, total foliar and root cardenolide concentration varied among milkweed species. Importantly, the effect of mycorrhizal fungi on total foliar cardenolide concentration also varied among milkweed species, with foliar cardenolides increasing or decreasing, depending on the plant species. We detected a phylogenetic signal to this variation; AMF fungi reduced foliar cardenolide concentrations to a greater extent in the clade including A. curassavica than in the clade including A. syriaca. Moreover, AMF inoculation shifted the composition of cardenolides in AG and BG plant tissues in a species-specific fashion. Mycorrhizal inoculation changed the relative distribution of cardenolides between root and shoot tissue in a species-specific fashion, but did not affect cardenolide diversity or polarity. Finally, a tradeoff between plant growth and defense in non-mycorrhizal plants was mitigated completely by AMF inoculation. Overall, we conclude that the effects of AMF inoculation on the expression of chemical resistance can vary among congeneric plant species, and ameliorate tradeoffs between growth and defense.

  12. Arbuscular mycorrhizal fungi alter above- and below-ground chemical defense expression differentially among Asclepias species

    PubMed Central

    Vannette, Rachel L.; Hunter, Mark D.; Rasmann, Sergio

    2013-01-01

    Below-ground (BG) symbionts of plants can have substantial influence on plant growth and nutrition. Recent work demonstrates that mycorrhizal fungi can affect plant resistance to herbivory and the performance of above- (AG) and BG herbivores. Although these examples emerge from diverse systems, it is unclear if plant species that express similar defensive traits respond similarly to fungal colonization, but comparative work may inform this question. To examine the effects of arbuscular mycorrhizal fungi (AMF) on the expression of chemical resistance, we inoculated 8 species of Asclepias (milkweed)—which all produce toxic cardenolides—with a community of AMF. We quantified plant biomass, foliar and root cardenolide concentration and composition, and assessed evidence for a growth-defense tradeoff in the presence and absence of AMF. As expected, total foliar and root cardenolide concentration varied among milkweed species. Importantly, the effect of mycorrhizal fungi on total foliar cardenolide concentration also varied among milkweed species, with foliar cardenolides increasing or decreasing, depending on the plant species. We detected a phylogenetic signal to this variation; AMF fungi reduced foliar cardenolide concentrations to a greater extent in the clade including A. curassavica than in the clade including A. syriaca. Moreover, AMF inoculation shifted the composition of cardenolides in AG and BG plant tissues in a species-specific fashion. Mycorrhizal inoculation changed the relative distribution of cardenolides between root and shoot tissue in a species-specific fashion, but did not affect cardenolide diversity or polarity. Finally, a tradeoff between plant growth and defense in non-mycorrhizal plants was mitigated completely by AMF inoculation. Overall, we conclude that the effects of AMF inoculation on the expression of chemical resistance can vary among congeneric plant species, and ameliorate tradeoffs between growth and defense. PMID:24065971

  13. Evaluation of plant species composition after thirteen years post coal mining rehabilitation in East Kutai District of East Kalimantan, Indonesia

    NASA Astrophysics Data System (ADS)

    Komara, L. L.; Murtinah, V.; Arbain

    2018-04-01

    Coal mining leaving large overburden which needs to be rehabilitated by conducting reclamation and re-vegetation. This study objective was to evaluate plant species composition after thirteen years of re-vegetation activities in a coal mining rehabilitation site in KutaiTimur District, East Kalimantan, with coordinate 00º33’23”-00º38’17” NL and 117º23’55”-117º23’20” EL. This study compared rehabilitation site with the natural forest conditions sites. There were found 28 plant species in the rehabilitation site, consisting of 19 wood species (Cassia siamea and Ficus uncinata) and 9 non-wood species (Nephrolepis biserata and Miscanthus javanica).In comparison, 36 species were found in the natural forest condition, consisting of 25 woody species (Nephelium eriopetalum and Macaranga hypoleuca)and 11 non-wood species (Fordia splendidisima and Saurauia umbellata). Woody species diversity indices in the rehabilitation site after 13 years post mining (i.e., 2,21) was lower than in the natural forest sites (3,01); while the diversity indices for non-wood species were relatively similar (1,48 and 1,96 in the rehabilitation and natural forest sites respectively). Species richness of non-wood species was low, but it has a high coverage per species in this site.To restore rehabilitation site woody species to its assumed natural conditions, 22woody species should be planted.

  14. Chapter 4: The Cretaceous-Lower Tertiary Composite Total Petroleum System, Wind River Basin, Wyoming

    USGS Publications Warehouse

    Johnson, R.C.; Finn, Thomas M.; Kirschbaum, Mark A.; Roberts, Stephen B.; Roberts, Laura N.R.; Cook, Troy; Taylor, David J.

    2007-01-01

    The Cretaceous-Lower Tertiary Composite Total Petroleum System (TPS) of the Wind River Basin Province includes all strata from the base of the Lower Cretaceous Cloverly Formation to the base of the Waltman Shale Member of the Paleocene age Fort Union Formation and, where the Waltman is absent, includes strata as young as the Eocene Wind River Formation. Locally, Cretaceous-sourced gas migrated into strata as old as the Mississippian Madison Limestone, and in these areas the TPS extends stratigraphically downward to include these reservoirs. The extensive vertical migration of gases in highly fractured areas of the Wind River Basin led to the commingling of gases from several Upper Cretaceous and lower Tertiary sources, thus only two petroleum systems are recognized in these rocks, the Cretaceous-Lower Tertiary Composite TPS, the subject of this report, and the Waltman Shale TPS described by Roberts and others (Chapter 5, this CD-ROM). The Cretaceous-lower Tertiary Composite TPS was subdivided into (1) seven continuous gas assessment units (AU): (a) Frontier-Muddy Continuous Gas AU, (b) Cody Sandstone Continuous Gas AU, (c) Mesaverde--Meeteetse Sandstone Gas AU, (d) Lance-Fort Union Sandstone Gas AU, (e) Mesaverde Coalbed Gas AU, (f) Meeteetse Coalbed Gas AU, and (g) Fort Union Coalbed Gas AU; (2) one continuous oil assessement unit--- Cody Fractured Shale Continuous Oil AU; and (3) one conventional assessment Unit--- Cretaceous-Tertiary Conventional Oil and Gas AU. Estimates of undiscovered resources having the potential for additions to reserves were made for all but the Cody Fractured Shale Continuous Oil AU, which is considered hypothetical and was not quantitively assessed. The mean estimate of the total oil is 41.99 million barrels, mean estimate of gas is 2.39 trillion cubic feet, and mean estimate of natural gas liquids is 20.55 million barrels. For gas, 480.66 billion cubic feet (BCFG) is estimated for the Frontier-Muddy Continuous Gas AU, 115.34 BCFG for

  15. Disentangling the Long-term Effects of Climate Change and Forest Structure and Species Composition on Streamflow Across the Eastern US

    NASA Astrophysics Data System (ADS)

    Caldwell, P.; Elliott, K.; Hartsell, A.; Miniat, C.

    2016-12-01

    Climate change and disturbances are threatening the ability of forested watersheds to provide the clean, reliable, and abundant fresh water necessary to support aquatic ecosystems and a growing human population. Forested watersheds in the eastern US have undergone significant change over the 20th century due to natural and introduced disturbances and a legacy of land use. We hypothesize that changes in forest age and species composition (i.e., forest change) associated with these disturbances may have altered forest water use and thus streamflow (Q) due to inherent differences in transpiration among species and forest ages. To test this hypothesis, we quantified changes in Q from 1960 to 2012 in 202 US Geological Survey forested reference watersheds across the eastern US, and separated the effect of changes in climate from forest change using Auto-Regressive Integrated Moving Average (ARIMA) time series modeling. We linked changes in Q to forest disturbance, forest ages and species composition using the Landsat-based North American Forest Dynamics dataset and plot-level USDA Forest Service Forest Inventory and Analysis (FIA) data. We found that 172 of the 202 sites (85%) exhibited changes in Q not accounted for by climate that we attributed to forest change and/or land use change. Among these, 76 (44%) had declining Q due to forest change (mostly in the southeastern US) while 96 (56%) had increasing Q (mostly in the mid-Atlantic and northeastern US). Across the 172 sites with forest-related changes in Q, 34% had at least 10% of the watershed area disturbed at least once from 1986-2010. In a case study of three watersheds, FIA data indicated that changes in forest structure and species composition explained observed changes in Q beyond climate effects. Our results suggest that forest-related changes in Q may have significant implications for water supply in the region and may inform forest management strategies to mitigate climate change impacts on water resources.

  16. Low melting high lithia glass compositions and methods

    DOEpatents

    Jantzen, Carol M.; Pickett, John B.; Cicero-Herman, Connie A.; Marra, James C.

    2003-09-23

    The invention relates to methods of vitrifying waste and for lowering the melting point of glass forming systems by including lithia formers in the glass forming composition in significant amounts, typically from about 0.16 wt % to about 11 wt %, based on the total glass forming oxides. The lithia is typically included as a replacement for alkali oxide glass formers that would normally be present in a particular glass forming system. Replacement can occur on a mole percent or weight percent basis, and typically results in a composition wherein lithia forms about 10 wt % to about 100 wt % of the alkali oxide glass formers present in the composition. The present invention also relates to the high lithia glass compositions formed by these methods. The invention is useful for stabilization of numerous types of waste materials, including aqueous waste uranium oxides The decrease in melting point achieved by the present invention desirably prevents volatilization of hazardous or radioactive species during vitrification.

  17. Plant Trait Variation along an Altitudinal Gradient in Mediterranean High Mountain Grasslands: Controlling the Species Turnover Effect

    PubMed Central

    Pescador, David S.; de Bello, Francesco; Valladares, Fernando; Escudero, Adrián

    2015-01-01

    Assessing changes in plant functional traits along gradients is useful for understanding the assembly of communities and their response to global and local environmental drivers. However, these changes may reflect the effects of species composition (i.e. composition turnover), species abundance (i.e. species interaction), and intra-specific trait variability (i.e. species plasticity). In order to determine the relevance of the latter, trait variation can be assessed under minimal effects of composition turnover. Nine sampling sites were established along an altitudinal gradient in a Mediterranean high mountain grassland community with low composition turnover (Madrid, Spain; 1940 m–2419 m). Nine functional traits were also measured for ten individuals of around ten plant species at each site, for a total of eleven species across all sites. The relative importance of different sources of variability (within/between site and intra-/inter-specific functional diversity) and trait variation at species and community level along the considered gradients were explored. We found a weak individual species response to altitude and other environmental variables although in some cases, individuals were smaller and leaves were thicker at higher elevations. This lack of species response was most likely due to greater within- than between-site species variation. At the community level, inter-specific functional diversity was generally greater than the intra-specific component except for traits linked to leaf element content (leaf carbon content, leaf nitrogen content, δ13C and δ15N). Inter-specific functional diversity decreased with lower altitude for four leaf traits (specific leaf area, leaf dry matter content, δ13C and δ15N), suggesting trait convergence between species at lower elevations, where water shortage may have a stronger environmental filtering effect than colder temperatures at higher altitudes. Our results suggest that, within a vegetation type encompassing

  18. Plant trait variation along an altitudinal gradient in mediterranean high mountain grasslands: controlling the species turnover effect.

    PubMed

    Pescador, David S; de Bello, Francesco; Valladares, Fernando; Escudero, Adrián

    2015-01-01

    Assessing changes in plant functional traits along gradients is useful for understanding the assembly of communities and their response to global and local environmental drivers. However, these changes may reflect the effects of species composition (i.e. composition turnover), species abundance (i.e. species interaction), and intra-specific trait variability (i.e. species plasticity). In order to determine the relevance of the latter, trait variation can be assessed under minimal effects of composition turnover. Nine sampling sites were established along an altitudinal gradient in a Mediterranean high mountain grassland community with low composition turnover (Madrid, Spain; 1940 m-2419 m). Nine functional traits were also measured for ten individuals of around ten plant species at each site, for a total of eleven species across all sites. The relative importance of different sources of variability (within/between site and intra-/inter-specific functional diversity) and trait variation at species and community level along the considered gradients were explored. We found a weak individual species response to altitude and other environmental variables although in some cases, individuals were smaller and leaves were thicker at higher elevations. This lack of species response was most likely due to greater within- than between-site species variation. At the community level, inter-specific functional diversity was generally greater than the intra-specific component except for traits linked to leaf element content (leaf carbon content, leaf nitrogen content, δ13C and δ15N). Inter-specific functional diversity decreased with lower altitude for four leaf traits (specific leaf area, leaf dry matter content, δ13C and δ15N), suggesting trait convergence between species at lower elevations, where water shortage may have a stronger environmental filtering effect than colder temperatures at higher altitudes. Our results suggest that, within a vegetation type encompassing

  19. Potential use of ionic species for identifying source land-uses of stormwater runoff.

    PubMed

    Lee, Dong Hoon; Kim, Jin Hwi; Mendoza, Joseph A; Lee, Chang-Hee; Kang, Joo-Hyon

    2017-02-01

    Identifying critical land-uses or source areas is important to prioritize resources for cost-effective stormwater management. This study investigated the use of information on ionic composition as a fingerprint to identify the source land-use of stormwater runoff. We used 12 ionic species in stormwater runoff monitored for a total of 20 storm events at five sites with different land-use compositions during the 2012-2014 wet seasons. A stepwise forward discriminant function analysis (DFA) with the jack-knifed cross validation approach was used to select ionic species that better discriminate the land-use of its source. Of the 12 ionic species, 9 species (K + , Mg 2+ , Na + , NH 4 + , Br - , Cl - , F - , NO 2 - , and SO 4 2- ) were selected for better performance of the DFA. The DFA successfully differentiated stormwater samples from urban, rural, and construction sites using concentrations of the ionic species (70%, 95%, and 91% of correct classification, respectively). Over 80% of the new data cases were correctly classified by the trained DFA model. When applied to data cases from a mixed land-use catchment and downstream, the DFA model showed the greater impact of urban areas and rural areas respectively in the earlier and later parts of a storm event.

  20. Chemical diversity in the genus Alpinia (Zingiberaceae): comparative composition of four Alpinia species grown in Northern India.

    PubMed

    Padalia, Rajendra C; Verma, Ram S; Sundaresan, Velusamy; Chanotiya, Chandan S

    2010-08-01

    The essential-oil compositions of leaves, flowers, and rhizomes of Alpinia galanga (L.) Willd., Alpinia calcarata Rosc., Alpinia speciosa K. Schum., and Alpinia allughas Rosc. were examined and compared by capillary GC and GC/MS. Monoterpenoids were the major oil constituents identified. 1,8-Cineole, alpha-terpineol, (E)-methyl cinnamate, camphor, terpinen-4-ol, and alpha- and beta-pinenes were the major constituents commonly distributed in leaf and flower essential oils. The presence of endo-fenchyl acetate, exo-fenchyl acetate, and endo-fenchol was the unique feature of rhizome essential oils of A. galanga, A. calcarata, and A. speciosa. On contrary, the rhizome oil of A. allughas was dominated by beta-pinene. Significant qualitative and quantitative variations were observed in essential-oil compositions of different parts of Alpinia species growing in subtemperate and subtropical regions of Northern India. Cluster analysis was performed to find similarities and differences in essential-oil compositions based on representative molecular skeletons. Monoterpenoids, viz., 1,8-cineole, terpinen-4-ol, camphor, pinenes, (E)-methyl cinnamate, and fenchyl derivatives, were used as chemotaxonomic markers.