Science.gov

Sample records for species file nos

  1. 78 FR 32622 - Endangered Species; File Nos. 17557 and 17273

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ... National Oceanic and Atmospheric Administration RIN 0648-XC703 Endangered Species; File Nos. 17557 and... National Ocean Service Marine Forensic Lab (NOS Lab) , 219 Fort Johnson Road, Charleston, SC 29412 (File No... Colligan], 1 Blackburn Drive, Gloucester, MA 01930 (File No. 17273), have applied in due form for...

  2. 77 FR 67631 - Endangered Species; File Nos. 17367 and 17364

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-13

    ... species recovery in four facilities in the Southeast Region of the USFWS. Research would include nutrition... National Oceanic and Atmospheric Administration RIN 0648-XC348 Endangered Species; File Nos. 17367 and... of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the...

  3. 76 FR 15300 - Endangered Species; File Nos. 16266 and 16291

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-21

    ... from the NMFS recovery plan outline for this species. The permits would not authorize any takes from... National Oceanic and Atmospheric Administration RIN 0648-XA306 Endangered Species; File Nos. 16266 and... requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et...

  4. 78 FR 50395 - Endangered Species; File Nos. 17557 and 17273

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... INFORMATION: On May 31, 2013, notice was published in the Federal Register (78 FR 32622) that requests for a... Service Marine Forensic Lab (NOS Lab) , 219 Fort Johnson Road, Charleston, SC 29412 (File No. 17557),...

  5. 76 FR 80890 - Endangered Species; File Nos. 13599 and 1614

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-27

    ...-01, issued on September 20, 2010 (73 FR 78724), authorizes the permit holder to receive, import...-01, (73 FR 25668) issued on April 30, 2008 authorizes the permit holder to collect, receive and... permits: the National Ocean Service Marine Forensic Lab (NOS Lab) , 219 Fort Johnson Road, Charleston,...

  6. 77 FR 21753 - Endangered Species; File Nos. 13599 and 1614

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    .... SUPPLEMENTARY INFORMATION: On December 27, 2011, notice was published in the Federal Register (76 FR 80890) that... and threatened species (50 CFR 222-226). Permit No. 13599, issued December 16, 2008 (73 FR 78724... mammal and endangered species parts. Permit No. 1614, issued February 28, 2008 (73 FR 11873),...

  7. 76 FR 37065 - Endangered Species; File Nos. 16266 and 16291

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    ... was published in the Federal Register (76 FR 15300) that a request for enhancement permits to take... Aquarium at Norwalk, 10 North Water Street, South Norwalk, CT 06854 have been issued permits to take... species (50 CFR Parts 222-226). The Virginia Living Museum and the Maritime Aquarium at Norwalk have...

  8. 77 FR 1061 - Endangered Species; File Nos. 16229 and 16548

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-09

    ... the Federal Register (76 FR 51945 and 76 FR 40699) that requests for enhancement permits to take... Zoo, 4401 Zoo ] Parkway, Asheboro, NC 27203 , and the Springfield Science Museum, 21 Edwards Street... species (50 CFR parts 222-226). The North Carolina Zoo and the Springfield Science Museum have been...

  9. 75 FR 16428 - Endangered Species; File Nos. 15112 and 13307-02

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... for purposes of scientific research. Kristen Hart, Ph.D., USGS, Davie, FL has applied for a modification to scientific research Permit No. 13307-01 to take green sea turtles. DATES: Written, telefaxed... species (50 CFR 222-226). File No. 15112: The purpose of the research is to determine the size...

  10. 75 FR 76704 - Endangered Species; File Nos. 13307, 13544, and 14586

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-09

    ... published in the Federal Register (75 FR 9580) that a request for a scientific research permit to take sea... for purposes of scientific research. ADDRESSES: The permit and related documents are available for..., FL (File No. 14586). On March 3, 2010, notice was published in the Federal Register (75 FR 9580)...

  11. 76 FR 18725 - Marine Mammals; File Nos. 14330 and 14335

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    ...Notice is hereby given that the following applicants have been issued major amendments to permits for research on marine mammals in Alaska: (File No. 14330) the Aleut Community of St. Paul Island, Tribal Government, Ecosystem Conservation Office, St. Paul Island, AK; and (File No. 14335) the Alaska SeaLife Center, Seward,...

  12. 75 FR 42689 - Marine Mammals; File Nos. 15498 and 15500

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ...: On May 3, 2010, notice was published in the Federal Register (75 FR 23242) that requests for public display permits to import bottlenose dolphins had been submitted by the above-named organizations. File No..., NW., Atlanta, GA 30313 have been issued permits to import Atlantic bottlenose dolphins...

  13. 76 FR 51001 - Marine Mammals; File Nos. 16109 and 15575

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-17

    ... 44 species to be targeted for research are listed as threatened or endangered: blue whale (Balaenoptera musculus), fin whale (B. physalus), humpback whale (Megaptera novaeangliae), North Atlantic right whale (Eubalaena glacialis), sei whale (B. borealis), sperm whale (Physeter macrocephalus), green...

  14. 77 FR 27719 - Marine Mammals; File Nos. 16109 and 15575

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... was published in the Federal Register (76 FR 51001) that requests for permits to conduct research on... targeted for research are listed as threatened or endangered: Blue whale (Balaenoptera musculus), fin whale... species to be targeted for research are listed as threatened or endangered: Blue whale, fin...

  15. 77 FR 67341 - Endangered Species; File No. 15809

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XC325 Endangered Species; File No. 15809 AGENCY... Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No..., Permits and Conservation Division By email to NMFS.Pr1Comments@noaa.gov (include the File No. in...

  16. 76 FR 51945 - Endangered Species; File No. 16548

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XA648 Endangered Species; File No. 16548 AGENCY... Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 16548 from the list of...- 0376, or by email to NMFS.Pr1Comments@noaa.gov . Please include the File No. 16548 in the subject...

  17. 78 FR 13642 - Endangered Species; File No. 17506

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XC518 Endangered Species; File No. 17506 AGENCY... Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 17506 from the list of...: By email to NMFS.Pr1Comments@noaa.gov (include the File No. in the subject line of the email),...

  18. 76 FR 76950 - Endangered Species; File No. 16134

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XA848 Endangered Species; File No. 16134 AGENCY... Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 16134 from the list of... Conservation Division. By email to NMFS.Pr1Comments@noaa.gov (include the File No. in the subject line of...

  19. 76 FR 51980 - Atlantic Shipping Company, Inc. v. Di Nos Shipping, Inc.; Notice of Filing of Complaint and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... From the Federal Register Online via the Government Publishing Office ] FEDERAL MARITIME COMMISSION Atlantic Shipping Company, Inc. v. Di Nos Shipping, Inc.; Notice of Filing of Complaint and Assignment Notice is given that a complaint has been filed with the Federal Maritime Commission (Commission) by Atlantic Shipping Company, Inc.,...

  20. 76 FR 37327 - Endangered Species; File No. 16253

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-27

    ... National Oceanic and Atmospheric Administration RIN 0648-XA518 Endangered Species; File No. 16253 AGENCY... requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 16253 from...

  1. 76 FR 48806 - Endangered Species; File No. 16194

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XA625 Endangered Species; File No. 16194 AGENCY... the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the... for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No....

  2. 75 FR 11862 - Endangered Species; File No. 14759

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XV04 Endangered Species; File No. 14759 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.), and the regulations... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov/ , and then selecting File No. 14759 from...

  3. 76 FR 27306 - Endangered Species; File No. 15661

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XA425 Endangered Species; File No. 15661 AGENCY... requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File...

  4. 75 FR 67682 - Endangered Species; File No. 15566

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-03

    ... National Oceanic and Atmospheric Administration RIN 0648-XA013 Endangered Species; File No. 15566 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15566 from...

  5. 77 FR 39220 - Endangered Species; File No. 17183

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-02

    ... National Oceanic and Atmospheric Administration RIN 0648-XC078 Endangered Species; File No. 17183 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No....

  6. 75 FR 9868 - Endangered Species; File No. 14622

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-04

    ... National Oceanic and Atmospheric Administration RIN 0648-XU82 Endangered Species; File No. 14622 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 14622 from...

  7. 78 FR 29114 - Endangered Species; File No. 17304

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-17

    ... National Oceanic and Atmospheric Administration RIN 0648-XC667 Endangered Species; File No. 17304 AGENCY... INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of 1973, as... Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 17304 from the list...

  8. 76 FR 35842 - Endangered Species; File No. 15685

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... National Oceanic and Atmospheric Administration RIN 0648-XA502 Endangered Species; File No. 15685 AGENCY... (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15685 from the list of..., Conservation and Education Division By e-mail to NMFS.Pr1Comments@noaa.gov (include the File No. in the...

  9. 77 FR 13096 - Endangered Species; File No. 16598

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... National Oceanic and Atmospheric Administration RIN 0648-XB054 Endangered Species; File No. 16598 AGENCY... page, https://apps.nmfs.noaa.gov , and then selecting File No. 16598 from the list of available... Division: By email to NMFS.Pr1Comments@noaa.gov (include the File No. in the subject line of the email),...

  10. 75 FR 13488 - Endangered Species; File No. 14949

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-22

    ... National Oceanic and Atmospheric Administration RIN 0648-XV33 Endangered Species; File No. 14949 AGENCY...://apps.nmfs.noaa.gov/ , and then selecting File No. 14949 from the list of available applications. These... the subject line of the e-mail comment the following document identifier: File No. 14949. FOR...

  11. 77 FR 58812 - Endangered Species; File No. 16733

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... National Oceanic and Atmospheric Administration RIN 0648-XC253 Endangered Species; File No. 16733 AGENCY....gov , and then selecting File No. 16733 from the list of available applications. These documents are...@noaa.gov (include the File No. in the subject line of the email), By facsimile to (301)713-0376, or...

  12. 77 FR 58812 - Endangered Species; File No. 17316

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... National Oceanic and Atmospheric Administration RIN 0648-XC252 Endangered Species; File No. 17316 AGENCY... page, https://apps.nmfs.noaa.gov , and then selecting File No. 17316 from the list of available... By email to NMFS.Pr1Comments@noaa.gov (include the File No. in the subject line of the email),...

  13. 76 FR 58471 - Endangered Species; File No. 15634

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ... National Oceanic and Atmospheric Administration RIN 0648-XA714 Endangered Species; File No. 15634 AGENCY....gov , and then selecting File No. 15634 from the list of available applications. These documents are..., Conservation and Education Division By e-mail to NMFS.Pr1Comments@noaa.gov (include the File No. in the...

  14. 76 FR 58471 - Endangered Species; File No. 16306

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ... National Oceanic and Atmospheric Administration RIN 0648-XA712 Endangered Species; File No. 16306 AGENCY... then selecting File No. 16306 from the list of available applications. These documents are also... the File No. in the subject line of the e-mail), By facsimile to (301) 713-0376, or At the...

  15. 75 FR 33578 - Endangered Species; File Nos. 14508 and 14655

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-14

    ... September 29, 2009, notice was published in the Federal Register (74 FR 38169 and 74 FR 49851) that requests...), Commerce. ACTION: Issuance of permits. SUMMARY: Notice is hereby given that Inwater Research Group, Inc... annually. Turtles may be flipper and passive integrated transponder tagged, blood and tissue...

  16. 77 FR 21750 - Endangered Species; File Nos. 16549 and 17095

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... permit for takes of shortnose sturgeon in the wild and captivity. The applicant proposes to determine up... animal research in laboratory tests of up- and downstream fish passage studies, swimming...

  17. 78 FR 17640 - Endangered Species; File Nos. 17367 and 17364

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-22

    ... FR 67631) that requests for scientific research permits to take shortnose sturgeon and/or Atlantic.... Research would include, but is not limited to, nutrition, physiology, propagation, contaminants, genetics... research animals for studies related to tagging, tracking, behavior, physiology, genetics,...

  18. 78 FR 38013 - Endangered Species; File No. 15661

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-25

    ... modification to Permit No. 15661, issued on January 24, 2012 (77 FR 13097) is requested under the authority of... National Oceanic and Atmospheric Administration RIN 0648-XA425 Endangered Species; File No. 15661 AGENCY... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov/ , and then selecting File No. 15661 from...

  19. 75 FR 13255 - Endangered Species; File No. 15338

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XU83 Endangered Species; File No. 15338 AGENCY... the Endangered Species Act of 1973, as amended (ESA). The Permit application is for the incidental... permits for threatened and endangered species are promulgated at 50 CFR 222.307. Species Covered in...

  20. 78 FR 41034 - Endangered Species; File No. 18102

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-09

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XC711 Endangered Species; File No. 18102 AGENCY...). Attachments to electronic comments will be accepted in Microsoft Word, Excel, or Adobe PDF file formats...

  1. 76 FR 77781 - Endangered Species; File No. 15802

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-14

    ... (50 CFR 222-226). On July 28, 2011 (76 FR 45230), notice was published that a permit had been... National Oceanic and Atmospheric Administration RIN 0648-XA603 Endangered Species; File No. 15802 AGENCY... change to the application for a permit (File No. 15802). DATES: Written, telefaxed, or email...

  2. 76 FR 23305 - Endangered Species; File No. 15672

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-26

    ... National Oceanic and Atmospheric Administration RIN 0648-XA385 Endangered Species; File No. 15672 AGENCY... subject permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16... the Features box on the Applications and Permits for Protected Species (APPS) home page,...

  3. 75 FR 11863 - Endangered Species; File No. 15135

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XV11 Endangered Species; File No. 15135 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the... Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and...

  4. 76 FR 44306 - Endangered Species; File No. 16146

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ... National Oceanic and Atmospheric Administration RIN 0648-XA583 Endangered Species; File No. 16146 AGENCY... under the ] authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and...

  5. 77 FR 37877 - Endangered Species; File No. 17022

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-25

    ... National Oceanic and Atmospheric Administration RIN 0648-XC059 Endangered Species; File No. 17022 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... Public Comment'' from the Features box on the Applications and Permits for Protected Species (APPS)...

  6. 75 FR 61424 - Endangered Species; File No. 15596

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... National Oceanic and Atmospheric Administration RIN 0648-XZ37 Endangered Species; File No. 15596 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226)....

  7. 76 FR 48146 - Endangered Species; File No. 1551

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-08

    ... National Oceanic and Atmospheric Administration RIN 0648-XA620 Endangered Species; File No. 1551 AGENCY.... 1551- 02 is requested under the authority of the Endangered Species Act of 1973, as amended (16 U.S.C... threatened species (50 CFR 222-226). Permit No. 1551, issued on July 24, 2008 (73 FR 44225),...

  8. 77 FR 31586 - Endangered Species; File No. 16556

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... National Oceanic and Atmospheric Administration RIN 0648-XC037 Endangered Species; File No. 16556 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... Public Comment'' from the Features box on the Applications and Permits for Protected Species (APPS)...

  9. 77 FR 21751 - Endangered Species; File No. 16645

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XB152 Endangered Species; File No. 16645 AGENCY... Natural Resources (GA DNR) has applied in due form for a permit pursuant to the Endangered Species Act of... following office: Endangered Species Division, Office of Protected Resources, NMFS, 1315 East-West...

  10. 76 FR 18725 - Endangered Species; File No. 16174

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    ... National Oceanic and Atmospheric Administration RIN 0648-XA348 Endangered Species; File No. 16174 AGENCY... subject permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16... box on the Applications and Permits for Protected Species (APPS) home page,...

  11. 75 FR 13256 - Endangered Species; File No. 14176

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XV28 Endangered Species; File No. 14176 AGENCY... permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C... Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov/ , and...

  12. 76 FR 45230 - Endangered Species; File No. 15802

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XA603 Endangered Species; File No. 15802 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... to take smalltooth sawfish and listed sea turtle species for purposes of scientific research....

  13. 76 FR 40699 - Endangered Species; File No. 16229

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XA516 Endangered Species; File No. 16229 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations... Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and...

  14. 75 FR 22106 - Endangered Species; File No. 14510

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-27

    ... National Oceanic and Atmospheric Administration RIN 0648-XW06 Endangered Species; File No. 14510 AGENCY... been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531...: On November 18, 2009, notice was published in the Federal Register (74 FR 59525) that a request for...

  15. 76 FR 32144 - Endangered Species; File No. 15677

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-03

    ... National Oceanic and Atmospheric Administration RIN 0648-XA467 Endangered Species; File No. 15677 AGENCY... permit has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S... 30, 2010, notice was published in the Federal Register (75 FR 74003) that a scientific...

  16. 78 FR 38952 - Endangered Species; File No. 17506

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XC518 Endangered Species; File No. 17506 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the..., notice was published in the Federal Register (78 FR 13642) that a request for a scientific...

  17. 75 FR 7443 - Endangered Species; File No. 14381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XU26 Endangered Species; File No. 14381 AGENCY... issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq...: On May 22, 2009, notice was published in the Federal Register (74 FR 23995) that a request for...

  18. 78 FR 3882 - Endangered Species; File No. 13543

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-17

    ... National Oceanic and Atmospheric Administration RIN 0648-XJ40 Endangered Species; File No. 13543 AGENCY.... SUPPLEMENTARY INFORMATION: The subject modification to Permit No. 13543, issued on May 6, 2009 (74 FR 20926) is requested under the authority of the Endangered Species Act of 1973, as amended (16 U.S.C. 1531 et seq.)...

  19. 78 FR 51712 - Endangered Species; File No. 16733

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-21

    ... National Oceanic and Atmospheric Administration RIN 0648-XC253 Endangered Species; File No. 16733 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the... published in the Federal Register (77 FR 58812) that a request for a scientific research permit to...

  20. 75 FR 21601 - Endangered Species; File No. 14604

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-26

    ... National Oceanic and Atmospheric Administration RIN 0648-XV94 Endangered Species; File No. 14604 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the... Register (74 FR 59961) that a request for a scientific research permit to take shortnose sturgeon had...

  1. 77 FR 10724 - Endangered Species; File No. 16253

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-23

    ... National Oceanic and Atmospheric Administration RIN 0648-XB027 Endangered Species; File No. 16253 AGENCY... permit has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.... SUPPLEMENTARY INFORMATION: On June 27, 2011, notice was published in the Federal Register (76 FR 37327) that...

  2. 75 FR 61133 - Endangered Species; File No. 14176

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-04

    ... National Oceanic and Atmospheric Administration RIN 0648-XZ31 Endangered Species; File No. 14176 AGENCY... issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... Register (75 FR 13256) that a request for a scientific research permit to take shortnose sturgeon had...

  3. 77 FR 1671 - Endangered Species; File No. 16194

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XA925 Endangered Species; File No. 16194 AGENCY... been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531..., 2011, notice was published in the ] Federal Register (76 FR 48806) that a request for a...

  4. 77 FR 57559 - Endangered Species; File No. 13330

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-18

    ... National Oceanic and Atmospheric Administration RIN 0648-XB144 Endangered Species; File No. 13330 AGENCY... modification has been granted under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U..., notice was published in the Federal Register (77 FR 19648) that a modification of Permit No....

  5. 75 FR 26715 - Endangered Species; File No. 1596

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XW36 Endangered Species; File No. 1596 AGENCY... modification to Permit No. 1596- 02, issued on July 29, 2009 (74 FR 38585), is requested under the authority of the Endangered Species Act of 1973, as amended (16 U.S.C. 1531 et seq.) and the regulations...

  6. 78 FR 5779 - Endangered Species; File No. 16248

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XC322 Endangered Species; File No. 16248 AGENCY... organization. The requested permit has been issued under the authority of the Endangered Species Act of 1973... INFORMATION: On October 30, 2012, notice was published in the Federal Register (77 FR 65673) that a...

  7. 76 FR 66042 - Endangered Species; File No. 1551

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ... National Oceanic and Atmospheric Administration RIN 0648-XA785 Endangered Species; File No. 1551 AGENCY... of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations... Register (76 FR 48146) that a modification of Permit No. 1551, issued July 24, 2008 (73 FR 44225) had...

  8. 78 FR 22517 - Endangered Species; File No. 16549

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-16

    ... National Oceanic and Atmospheric Administration RIN 0648-XB155 Endangered Species; File No. 16549 AGENCY... applicant. The requested permit has been issued under the authority of the Endangered Species Act of 1973.... SUPPLEMENTARY INFORMATION: On April 11, 2012, notice was published in the Federal Register (77 FR 21750) that...

  9. 78 FR 22239 - Endangered Species; File No. 16556

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-15

    .... SUPPLEMENTARY INFORMATION: On May 29, 2012, notice was published in the Federal Register (77 FR 31586) that a... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XC037 Endangered Species; File No. 16556...

  10. 78 FR 59657 - Endangered Species; File No. 17304

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ... the Federal Register (78 FR 29114) that a request for a scientific research permit to take loggerhead... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XC667 Endangered Species; File No. 17304...

  11. 76 FR 22677 - Endangered Species; File No. 14949

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-22

    ... Federal Register (75 FR 13488) that a request for a scientific research permit to take green and hawksbill... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XA382 Endangered Species; File No. 14949...

  12. 77 FR 24686 - Endangered Species; File No. 15634

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-25

    ... Register (76 FR 58471) that a request for a scientific research permit to take leatherback sea turtles had... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XA714 Endangered Species; File No. 15634...

  13. 76 FR 22877 - Endangered Species; File No. 15566

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-25

    .... SUPPLEMENTARY INFORMATION: On November 3, 2010, notice was published in the Federal Register (75 FR 67682) that... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XA387 Endangered Species; File No. 15566...

  14. 76 FR 45781 - Endangered Species; File No. 15552

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... INFORMATION: On January 26, 2011, notice was published in the Federal Register (76 FR 4636) that a request for... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XA601 Endangered Species; File No. 15552...

  15. 78 FR 31519 - Endangered Species; File No. 13543

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ... was published in the Federal Register (78 FR 3882) that a modification to Permit No. 13543, issued April 30, 2009 (74 FR 20926), had been requested by the above-named organization. The requested... National Oceanic and Atmospheric Administration RIN 0648-XJ40 Endangered Species; File No. 13543...

  16. 78 FR 2659 - Endangered Species; File No. 16645

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-14

    ... INFORMATION: On April 11, 2012, notice was published in the Federal Register (77 FR 21751) that a request for... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XB152 Endangered Species; File No. 16645...

  17. 75 FR 78227 - Endangered Species; File No. 14400

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-15

    ... INFORMATION: On August 27, 2009, notice was published in the Federal Register (74 FR 43679) that a request for... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XA085 Endangered Species; File No. 14400...

  18. 77 FR 40588 - Endangered Species; File No. 16598

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-10

    .... SUPPLEMENTARY INFORMATION: On March 5, 2012, notice was published in the Federal Register (77 FR 13096) that a... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XB054 Endangered Species; File No. 16598...

  19. 78 FR 50396 - Endangered Species; File No. 17405

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... Register (78 FR 14078) that a request for a scientific research permit to take black abalone had been... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XC519 Endangered Species; File No. 17405...

  20. 75 FR 53278 - Endangered Species; File No. 14759

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    .... SUPPLEMENTARY INFORMATION: On March 12, 2010, notice was published in the Federal Register (75 FR 11862) that a... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XY58 Endangered Species; File No. 14759...

  1. 78 FR 26323 - Endangered Species; File No. 17183

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-06

    ...-8401. SUPPLEMENTARY INFORMATION: On July 2, 2012, notice was published in the Federal Register (77 FR... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XC078 Endangered Species; File No. 17183...

  2. 77 FR 55194 - Endangered Species; File No. 17095

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-07

    ... Federal Register (77 FR 21750) that a request for a scientific research permit to take shortnose sturgeon... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XB155 Endangered Species; File No. 17095...

  3. 78 FR 57132 - Endangered Species; File No. 16230

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-17

    ... and a request for public comments on October 5, 2011 (76 FR 61670). Based on comments received from... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XC289 Endangered Species; File No. 16230...

  4. 77 FR 61745 - Endangered Species; File No. 16803

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-11

    ... Register (77 FR 34349) that a request for a scientific research permit to take loggerhead, green, and olive... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XC058 Endangered Species; File No. 16803...

  5. 76 FR 77780 - Endangered Species; File No. 10022

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-14

    ... FR 78974) that a modification of Permit No. 10022-01, issued May 12, 2010 (75 FR 26715), had been... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XA867 Endangered Species; File No. 10022...

  6. 76 FR 19052 - Endangered Species; File No. 14344

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ... National Oceanic and Atmospheric Administration RIN 0648-XA340 Endangered Species; File No. 14344 AGENCY... above-named organization. The requested permit has been issued under the authority of the Endangered.... SUPPLEMENTARY INFORMATION: On August 27, 2009, notice was published in the Federal Register (74 FR 43679) that...

  7. 76 FR 68161 - Marine Mammals; File Nos. 16163, 16160, and 15569

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-03

    ... are listed as endangered or have a stock listed as endangered: blue whale (Balaenoptera musculus), fin... species that are listed as endangered include the blue whale, fin whale, sei whale, humpback whale, and...], 2725 Montlake Blvd. East, Seattle, WA 98112-2097; The Whale Museum (Jenny Atkinson, Responsible...

  8. 78 FR 2955 - Marine Mammals; File Nos. 14451, 14353, and 13846

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... species (50 CFR 222-226). Permit No. 14451, issued on July 14, 2010 (75 FR 43151), authorizes the permit..., issued on July 14, 2010 (75 FR 43151), authorizes the permit holder, Ann Zoidis, Cetos Research... the Northwestern Hawaiian Islands (NWHI) chain. Permit No. 13846, issued on July 14, 2010 (75 FR...

  9. 75 FR 60734 - Endangered Species; File No. 13599-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-01

    .... SUPPLEMENTARY INFORMATION: On May 25, 2010, notice was published in the Federal Register (75 FR 29316) that a modification of Permit No. 13599, ] issued December 16, 2008 (73 FR 78724), had been requested by the above... Service Marine Forensic Lab (NOS Lab, Julie Carter, Principal Investigator), 219 Fort Johnson...

  10. 76 FR 23571 - Marine Mammals and Endangered Species; File Nos. 15415 and 14622

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-27

    ... December 10, 2010, notice was published in the Federal Register (75 FR 76956) that a request for a permit... D. Kraus, Ph.D. . On March 4, 2010, notice was published in the Federal Register (75 FR 9868) that a... given that NMFS has issued two permits to conduct research on marine mammals or sea turtles....

  11. 78 FR 23225 - Endangered Species; File Nos. 14759-01 and 16375-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-18

    ... issued August 20, 2010 (75 FR 53278), and Permit No. 16375 was issued on April 6, 2012 (77 FR 21754) to... is now requesting authorization to modify both permits to allow use of artificial substrates for... artificial substrates be used for collecting up to 50 shortnose sturgeon and 50 Atlantic sturgeon early...

  12. 76 FR 58469 - Endangered Species; File Nos. 16526, 16323, 16436, 16422, 16438, 16431, 16507, 16547, 16375...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ... below. For specific take numbers of each research project, please refer to the associated application..., movement, habitat preferences and biology in the Delaware River and Bay. The applicant would...

  13. 77 FR 13097 - Endangered Species; File Nos. 15661, 10027, and 15685

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... Federal Register (76 FR 27306) that a request for a scientific research permit to take green and hawksbill... Federal Register (76 FR 34967) that a request for a scientific research permit modification to take green... in the Federal Register (76 FR 35842) that a request for a scientific research permit to take...

  14. 77 FR 21754 - Endangered Species; File Nos. 16526, 16323, 16436, 16422, 16438, 16431, 16507, 16547, 16375...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... 21, 2011, notice was published in the Federal Register (76 FR 58469) that 12 requests for scientific... Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) for purposes of scientific research. See SUPPLEMENTARY... five-year permit to conduct scientific research on Atlantic sturgeon in the rivers and estuaries...

  15. 75 FR 9580 - Marine Mammals and Endangered Species; File Nos. 13544 and 14586

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-03

    ..., Naples, FL 34102, has requested a modification to scientific research Permit No. 13544 and Jeanette Wyneken, Florida Atlantic University, Boca Raton, FL 33431, has applied in due form to conduct scientific... Permit No. 13544, issued on April 17, 2009 (74 FR 18354) is requested under the authority of...

  16. 76 FR 34967 - Endangered Species; File No. 10027

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    .... 10027, issued on July 30, 2008 (73 FR 44224), is requested under the authority of the Endangered Species...: Eleanor Sterling, PhD), Central Park West at 79th Street, New York, New York 10024, has requested...

  17. 76 FR 6118 - Endangered Species; File No. 14726

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ... threatened species (50 CFR 222-226). Permit No. 14726, issued on September 3, 2010 (75 FR 61133), authorizes... Blair Witherington, PhD, Florida Fish and Wildlife Conservation Commission, 9700 South A1A,...

  18. 78 FR 66901 - Endangered Species; File No. 16482-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-07

    ...) and shortnose sturgeon (Acipenser brevirostrum) for purposes of scientific research. DATES: Written... threatened species (50 CFR parts 222-226). Permit No. 16482 was issued April 6, 2012 (77 FR 21754) to...

  19. 75 FR 61133 - Marine Mammals and Endangered Species; File Nos. 808-1735, 14233, 14506, 14603, and 14726

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-04

    .... SUPPLEMENTARY INFORMATION: On March 11, 2010, notice was published in the Federal Register (75 FR 11516) that a... submitted by Andrew Read, Ph.D. . On September 10, 2009, notice was published in the Federal Register (74 FR... Federal Register (74 FR 66618) that a request for a permit to conduct research on green (Chelonia...

  20. 76 FR 30309 - Marine Mammals and Endangered Species; File Nos. 14245, 1596-03, and 14726-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ..., 2010 and December 29, 2010, notices were published in the Federal Register (75 FR 26715 and 81970.... On May 12, 2010, notice was published in the Federal Register (75 FR 26715) that a modification of... Beach, FL, 32951 was issued a modification to Permit No. 14726, issued September 3, 2010 (75 FR...

  1. Reactive oxygen species- and nitric oxide-mediated lung inflammation and mitochondrial dysfunction in wild-type and iNOS-deficient mice exposed to diesel exhaust particles.

    PubMed

    Zhao, Hongwen; Ma, Joseph K; Barger, Mark W; Mercer, Robert R; Millecchia, Lyndell; Schwegler-Berry, Diane; Castranova, Vince; Ma, Jane Y

    2009-01-01

    Pulmonary responses to diesel exhaust particles (DEP) exposure are mediated through enhanced production of reactive oxygen species (ROS) and nitric oxide (NO) by alveolar macrophages (AM). The current study examined the differential roles of ROS and NO in DEP-induced lung injury using C57B/6J wild-type (WT) and inducible NO synthase knockout (iNOS KO) mice. Mice exposed by pharyngeal aspiration to DEP or carbon black particles (CB) (35 mg/kg) showed an inflammatory profile that included neutrophil infiltration, increased lactate dehydrogenase (LDH) activity, and elevated albumin content in bronchoalveolar lavage fluid (BALF) at 1, 3, and 7 d postexposure. The organic extract of DEP (DEPE) did not induce an inflammatory response. Comparing WT to iNOS KO mice, the results show that NO enhanced DEP-induced neutrophils infiltration and plasma albumin content in BALF and upregulated the production of the pro-inflammatory cytokine interleukin 12 (IL-12) by AM. DEP-exposed AM from iNOS KO mice displayed diminished production of IL-12 and, in response to ex vivo lipopolysaccharide (LPS) challenge, decreased production of IL-12 but increased production of IL-10 when compared to cells from WT mice. DEP, CB, but not DEPE, induced DNA damage and mitochondria dysfunction in AM, however, that is independent of cellular production of NO. These results demonstrate that DEP-induced immune/inflammatory responses in mice are regulated by both ROS- and NO-mediated pathways. NO did not affect ROS-mediated mitochondrial dysfunction and DNA damage but upregulated IL-12 and provided a counterbalance to the ROS-mediated adaptive stress response that downregulates IL-12 and upregulates IL-10. PMID:19267316

  2. Permanent-File-Validation Utility Computer Program

    NASA Technical Reports Server (NTRS)

    Derry, Stephen D.

    1988-01-01

    Errors in files detected and corrected during operation. Permanent File Validation (PFVAL) utility computer program provides CDC CYBER NOS sites with mechanism to verify integrity of permanent file base. Locates and identifies permanent file errors in Mass Storage Table (MST) and Track Reservation Table (TRT), in permanent file catalog entries (PFC's) in permit sectors, and in disk sector linkage. All detected errors written to listing file and system and job day files. Program operates by reading system tables , catalog track, permit sectors, and disk linkage bytes to vaidate expected and actual file linkages. Used extensively to identify and locate errors in permanent files and enable online correction, reducing computer-system downtime.

  3. 76 FR 39910 - Nine Mile Point Nuclear Station, LLC; Nine Mile Point Nuclear Station, Unit Nos. 1 and 2; Notice...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ... accordance with the NRC E-Filing rule (72 FR 49139, August 28, 2007). The E-Filing process requires... COMMISSION Nine Mile Point Nuclear Station, LLC; Nine Mile Point Nuclear Station, Unit Nos. 1 and 2; Notice... Nos. DPR-63 and NPF-69 for the Nine Mile Point Nuclear Station, Unit Nos. 1 and 2 (NMP),...

  4. 76 FR 64937 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-19

    ... Company. Description: Notices of Termination of CalPeak Vaca Dixon and Panoche Service Agreement Nos. 39...: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866)...

  5. 76 FR 51447 - Self-Regulatory Organizations; Chicago Stock Exchange, Inc.; Notice of Filing of Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... Exchange Act Release Nos. 62252 (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014...-047); 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010- 025). \\7\\ See e.g.,Securities Exchange Act Release Nos. 62884 (September 10, 2010), 75 FR 56618 (September 16, 2010) (File Nos....

  6. 76 FR 39908 - Calvert Cliffs Nuclear Power Plant, LLC; Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and 2...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ... participating under 10 CFR 2.315(c), must be filed in accordance with the NRC E-Filing rule (72 FR 49139, August... COMMISSION Calvert Cliffs Nuclear Power Plant, LLC; Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and 2.... DPR-53 and DPR-69, for the Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and 2 (CCNPP),...

  7. mNos2 Deletion and Human NOS2 Replacement in Alzheimer Disease Models

    PubMed Central

    Colton, Carol A.; Wilson, Joan G.; Everhart, Angela; Wilcock, Donna M.; Puoliväli, Jukka; Heikkinen, Taneli; Oksman, Juho; Jääskeläinen, Olli; Lehtimäki, Kimmo; Laitinen, Teemu; Vartiainen, Nina; Vitek, Michael P.

    2014-01-01

    Abstract Understanding the pathophysiologic mechanisms underlying Alzheimer disease relies on knowledge of disease onset and the sequence of development of brain pathologies. We present a comprehensive analysis of early and progressive changes in a mouse model that demonstrates a full spectrum of characteristic Alzheimer disease–like pathologies. This model demonstrates an altered immune redox state reminiscent of the human disease and capitalizes on data indicating critical differences between human and mouse immune responses, particularly in nitric oxide levels produced by immune activation of the NOS2 gene. Using the APPSwDI+/+/mNos2−/− (CVN-AD) mouse strain, we show a sequence of pathologic events leading to neurodegeneration,which include pathologically hyperphosphorylated tau in the perforant pathway at 6 weeks of age progressing to insoluble tau, early appearance of β-amyloid peptides in perivascular deposits around blood vessels in brain regions known to be vulnerable to Alzheimer disease, and progression to damage and overt loss in select vulnerable neuronal populations in these regions. The role of species differences between hNOS2 and mNos2 was supported by generating mice in which the human NOS2 gene replaced mNos2. When crossed with CVN-AD mice, pathologic characteristics of this new strain (APPSwDI+/−/HuNOS2tg+/+/mNos2−/−) mimicked the pathologic phenotypes found in the CVN-AD strain. PMID:25003233

  8. mNos2 deletion and human NOS2 replacement in Alzheimer disease models.

    PubMed

    Colton, Carol A; Wilson, Joan G; Everhart, Angela; Wilcock, Donna M; Puoliväli, Jukka; Heikkinen, Taneli; Oksman, Juho; Jääskeläinen, Olli; Lehtimäki, Kimmo; Laitinen, Teemu; Vartiainen, Nina; Vitek, Michael P

    2014-08-01

    Understanding the pathophysiologic mechanisms underlying Alzheimer disease relies on knowledge of disease onset and the sequence of development of brain pathologies. We present a comprehensive analysis of early and progressive changes in a mouse model that demonstrates a full spectrum of characteristic Alzheimer disease-like pathologies. This model demonstrates an altered immune redox state reminiscent of the human disease and capitalizes on data indicating critical differences between human and mouse immune responses, particularly in nitric oxide levels produced by immune activation of the NOS2 gene. Using the APPSwDI(+)/(+)mNos2(-/-) (CVN-AD) mouse strain, we show a sequence of pathologic events leading to neurodegeneration,which include pathologically hyperphosphorylated tau in the perforant pathway at 6 weeks of age progressing to insoluble tau, early appearance of β-amyloid peptides in perivascular deposits around blood vessels in brain regions known to be vulnerable to Alzheimer disease, and progression to damage and overt loss in select vulnerable neuronal populations in these regions. The role of species differences between hNOS2 and mNos2 was supported by generating mice in which the human NOS2 gene replaced mNos2. When crossed with CVN-AD mice, pathologic characteristics of this new strain (APPSwDI(+)/(-)/HuNOS2(tg+)/(+)/mNos2(-/-)) mimicked the pathologic phenotypes found in the CVN-AD strain. PMID:25003233

  9. 77 FR 17470 - Notice of Compliance Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Compliance Filing Docket Nos. OREG 1, Inc EL11-22-000 OREG 2, Inc...., OREG 2, Inc., OREG 3, Inc., and OREG 4, Inc filed compliance refund reports, pursuant to the...

  10. 15. Dry Dock No. 4. Longitudinal Section. Subdivision Nos. I ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Dry Dock No. 4. Longitudinal Section. Subdivision Nos. I and II (Frederic R. Harris, Inc., January 10, 1941). In Files of Cushman & Wakefield, Building no. 501, Philadelphia Naval Business Center. - Naval Base Philadelphia-Philadelphia Naval Shipyard, Dry Dock No. 4, Broad Street south of Government Avenue, Philadelphia, Philadelphia County, PA

  11. Service building. Cross section thru dry dock nos. 4 & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Service building. Cross section thru dry dock nos. 4 & 5 showing service bldg & 20-75-150 ton cranes (dry dock associates, May 23, 1941). In files of Cushman & Wakefield, building no. 501, Philadelphia Naval Business Center. - Naval Base Philadelphia-Philadelphia Naval Shipyard, Service Building, Dry Docks No. 4 & 5, League Island, Philadelphia, Philadelphia County, PA

  12. Langley VEDIT for NOS/VE usage manual

    NASA Technical Reports Server (NTRS)

    Heaney, Margaret A.

    1987-01-01

    The use of the VEDIT editor on the CDC Network Operating System/Virtual Environment (NOS/VE) is discussed. The VEDIT, a utility, allows a user to edit files line by line (line mode). How to access and the use of VEDIT are explained. The parameters and the format of the individual commands are defined. Examples are included.

  13. 16. Dry Dock No. 4. Longitudinal Section. Subdivision Nos. III ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Dry Dock No. 4. Longitudinal Section. Subdivision Nos. III and IV (Frederic R. Harris, Inc., January 10, 1941). In Files of Cushman & Wakefield, Building no. 501, Philadelphia Naval Business Center. - Naval Base Philadelphia-Philadelphia Naval Shipyard, Dry Dock No. 4, Broad Street south of Government Avenue, Philadelphia, Philadelphia County, PA

  14. The NOS Challenge

    ERIC Educational Resources Information Center

    Quigley, Cassie; Buck, Gayle; Akerson, Valarie

    2011-01-01

    "The picture of a scientist is me!" exclaims first grader Kendra during a nature of science (NOS) lesson. She drew a picture of a scientist and explained that she was going to be a scientist when she grew up because she "loved to observe like a scientist." Kendra's experience was a part of a 30-day unit designed specifically for first graders.…

  15. 78 FR 52598 - Self-Regulatory Organizations; Fixed Income Clearing Corporation; Notice of Filing of Proposed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-23

    ... COMMISSION Self-Regulatory Organizations; Fixed Income Clearing Corporation; Notice of Filing of Proposed..., the Fixed Income Clearing Corporation (``FICC'') filed with the Securities and Exchange Commission...) the counterparty submits corresponding NOS to match the initiator's submission, (b) the...

  16. 76 FR 51089 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing of Proposed Rule Change To...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-17

    ... FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014; SR-EDGA- 2010-01; SR-EDGX-2010-01; SR-BX-2010... 10, 2010), 75 FR 56618 (September 16, 2010) (File Nos. SR-BATS-2010-018; SR-BX-2010-044; SR-CBOE-2010... (June 23, 2011), 76 FR 38243 (June 29, 2011) (File Nos. SR-BATS-2011-016; SR-BYX- 2011-011;...

  17. 75 FR 3458 - Combined Notice of Filings # 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-21

    ..., January 28, 2010. Docket Numbers: OA10-5-000. Applicants: ConocoPhillips Company. Description: Request for Waiver of Order Nos. 888, 889, and 890 and Part 358 of ConocoPhillips Company. Filed Date:...

  18. 76 FR 74086 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-30

    ... Exchange Act Release No. 62252 (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS- 2010-014...) and Securities Exchange Act Release No. 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA... Exchange Act Release No. 62884 (September 10, 2010), 75 FR 56618 (September 16, 2010) (File Nos....

  19. 76 FR 2174 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-12

    ... Securities Exchange Act Release No. 35993 (July 19, 1995), 60 FR 38073 (July 25, 1995) (approving File Nos..., 1998), 63 FR 64297 (November 19, 1998) (approving File Nos. SR-Amex-98-21, SR-CBOE-98-29, SR-PCX-98- 31... Price Program. See Securities Exchange Act Release No. 52960 (December 15, 2005), 70 FR 76090...

  20. XML Files

    MedlinePlus

    ... nlm.nih.gov/medlineplus/xml.html MedlinePlus XML Files To use the sharing features on this page, ... information on all English and Spanish topic groups. Files generated on July 09, 2016 MedlinePlus Health Topic ...

  1. Contribution of the nos-pdt operon to virulence phenotypes in methicillin-sensitive Staphylococcus aureus.

    PubMed

    Sapp, April M; Mogen, Austin B; Almand, Erin A; Rivera, Frances E; Shaw, Lindsey N; Richardson, Anthony R; Rice, Kelly C

    2014-01-01

    Nitric oxide (NO) is emerging as an important regulator of bacterial stress resistance, biofilm development, and virulence. One potential source of endogenous NO production in the pathogen Staphylococcus aureus is its NO-synthase (saNOS) enzyme, encoded by the nos gene. Although a role for saNOS in oxidative stress resistance, antibiotic resistance, and virulence has been recently-described, insights into the regulation of nos expression and saNOS enzyme activity remain elusive. To this end, transcriptional analysis of the nos gene in S. aureus strain UAMS-1 was performed, which revealed that nos expression increases during low-oxygen growth and is growth-phase dependent. Furthermore, nos is co-transcribed with a downstream gene, designated pdt, which encodes a prephenate dehydratase (PDT) enzyme involved in phenylalanine biosynthesis. Deletion of pdt significantly impaired the ability of UAMS-1 to grow in chemically-defined media lacking phenylalanine, confirming the function of this enzyme. Bioinformatics analysis revealed that the operon organization of nos-pdt appears to be unique to the staphylococci. As described for other S. aureus nos mutants, inactivation of nos in UAMS-1 conferred sensitivity to oxidative stress, while deletion of pdt did not affect this phenotype. The nos mutant also displayed reduced virulence in a murine sepsis infection model, and increased carotenoid pigmentation when cultured on agar plates, both previously-undescribed nos mutant phenotypes. Utilizing the fluorescent stain 4-Amino-5-Methylamino-2',7'-Difluorofluorescein (DAF-FM) diacetate, decreased levels of intracellular NO/reactive nitrogen species (RNS) were detected in the nos mutant on agar plates. These results reinforce the important role of saNOS in S. aureus physiology and virulence, and have identified an in vitro growth condition under which saNOS activity appears to be upregulated. However, the significance of the operon organization of nos-pdt and potential

  2. Contribution of the nos-pdt Operon to Virulence Phenotypes in Methicillin-Sensitive Staphylococcus aureus

    PubMed Central

    Almand, Erin A.; Rivera, Frances E.; Shaw, Lindsey N.; Richardson, Anthony R.; Rice, Kelly C.

    2014-01-01

    Nitric oxide (NO) is emerging as an important regulator of bacterial stress resistance, biofilm development, and virulence. One potential source of endogenous NO production in the pathogen Staphylococcus aureus is its NO-synthase (saNOS) enzyme, encoded by the nos gene. Although a role for saNOS in oxidative stress resistance, antibiotic resistance, and virulence has been recently-described, insights into the regulation of nos expression and saNOS enzyme activity remain elusive. To this end, transcriptional analysis of the nos gene in S. aureus strain UAMS-1 was performed, which revealed that nos expression increases during low-oxygen growth and is growth-phase dependent. Furthermore, nos is co-transcribed with a downstream gene, designated pdt, which encodes a prephenate dehydratase (PDT) enzyme involved in phenylalanine biosynthesis. Deletion of pdt significantly impaired the ability of UAMS-1 to grow in chemically-defined media lacking phenylalanine, confirming the function of this enzyme. Bioinformatics analysis revealed that the operon organization of nos-pdt appears to be unique to the staphylococci. As described for other S. aureus nos mutants, inactivation of nos in UAMS-1 conferred sensitivity to oxidative stress, while deletion of pdt did not affect this phenotype. The nos mutant also displayed reduced virulence in a murine sepsis infection model, and increased carotenoid pigmentation when cultured on agar plates, both previously-undescribed nos mutant phenotypes. Utilizing the fluorescent stain 4-Amino-5-Methylamino-2',7'-Difluorofluorescein (DAF-FM) diacetate, decreased levels of intracellular NO/reactive nitrogen species (RNS) were detected in the nos mutant on agar plates. These results reinforce the important role of saNOS in S. aureus physiology and virulence, and have identified an in vitro growth condition under which saNOS activity appears to be upregulated. However, the significance of the operon organization of nos-pdt and potential

  3. 76 FR 10344 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    .... 310 between APS and Perrin Ranch Wind, LLC. to be effective 1/13/2011. Filed Date: 02/14/2011... above list. They are also available for review in the Commission's Public Reference Room in Washington... tariff filing per 35.13(a)(2)(iii): Amendments to Solar Partners LGIAs SA Nos. 73, 78 and 85 to...

  4. Therapeutic effect of enhancing endothelial nitric oxide synthase (eNOS) expression and preventing eNOS uncoupling

    PubMed Central

    Förstermann, Ulrich; Li, Huige

    2011-01-01

    Nitric oxide (NO) produced by the endothelium is an important protective molecule in the vasculature. It is generated by the enzyme endothelial NO synthase (eNOS). Similar to all NOS isoforms, functional eNOS transfers electrons from nicotinamide adenine dinucleotide phosphate (NADPH), via the flavins flavin adenine dinucleotide and flavin mononucleotide in the carboxy-terminal reductase domain, to the heme in the amino-terminal oxygenase domain. Here, the substrate L-arginine is oxidized to L-citrulline and NO. Cardiovascular risk factors such as diabetes mellitus, hypertension, hypercholesterolaemia or cigarette smoking reduce bioactive NO. These risk factors lead to an enhanced production of reactive oxygen species (ROS) in the vessel wall. NADPH oxidases represent major sources of this ROS and have been found upregulated in the presence of cardiovascular risk factors. NADPH-oxidase-derived superoxide avidly reacts with eNOS-derived NO to form peroxynitrite (ONOO-). The essential NOS cofactor (6R-)5,6,7,8-tetrahydrobiopterin (BH4) is highly sensitive to oxidation by this ONOO-. In BH4 deficiency, oxygen reduction uncouples from NO synthesis, thereby converting NOS to a superoxide-producing enzyme. Among conventional drugs, compounds interfering with the renin-angiotensin-aldosterone system and statins can reduce vascular oxidative stress and increase bioactive NO. In recent years, we have identified a number of small molecules that have the potential to prevent eNOS uncoupling and, at the same time, enhance eNOS expression. These include the protein kinase C inhibitor midostaurin, the pentacyclic triterpenoids ursolic acid and betulinic acid, the eNOS enhancing compounds AVE9488 and AVE3085, and the polyphenolic phytoalexin trans-resveratrol. Such compounds enhance NO production from eNOS also under pathophysiological conditions and may thus have therapeutic potential. PMID:21198553

  5. 76 FR 17411 - Notice of Intent To File License Application, Filing of Pre-Application Document, and Approving...

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    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Intent To File License Application, Filing of Pre- Application Document, and Approving Use of the Traditional Licensing Process Project Nos. Free Flow Power Corporation P-13441-001 P-13456-001 P-13455-001...

  6. 75 FR 23242 - Marine Mammals; File Nos. 15498 and 15500

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    ..., Atlanta, GA 30313, have each applied in due form for a permit to import Atlantic bottlenose dolphins... female captive born bottlenose dolphin from Dolphin Quest Bermuda, Hamilton, Bermuda, to the Brookfield... dolphins from Dolphin Experience, Ltd., Freeport, Grand Bahama Island, The Bahamas, and three...

  7. 78 FR 21112 - Marine Mammals; File Nos. 16992 and 14535

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    ... amendment to Permit No. 14535-01 (75 FR 58352) to allow the addition of TTS studies to the currently..., Ph.D., Hawaii Institute of Marine Biology, University of Hawaii, P.O. Box 1106, Kailua, HI 96734, has... of Marine Biology in Kaneohe, HI. Researchers would conduct hearing measurements using suction...

  8. 78 FR 60852 - Marine Mammals; File Nos. 16239 and 17312

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  9. 75 FR 37389 - Marine Mammals; File Nos. 14628 and 15471

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    ... purposes:National Museum of Natural History, Smithsonian Institution (Charles W. Potter, Responsible Party... Museum of Natural History (NMNH) is requesting authorization to collect, import, export, possess,...

  10. 77 FR 64959 - Marine Mammals; File Nos. 15777 and 17670

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-24

    ...) was made available for public comment on May 4, 2012 (77 FR 26513). In response to public comments..., hair, swab samples, vibrissae), and attachment of telemetry devices. Up to 200 harp seals, 50...

  11. 76 FR 9384 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

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    ...), 75 FR 69489 (November 12, 2010)(NASDAQ-2010-141) (notice of filing and immediate effectiveness)(the ``ISP Filing''). See also Securities Exchange Act Release Nos. 63414 (December 2, 2010), 75 FR 76505..., 2011), 76 FR 1201 (January 7, 2011)(NASDAQ-2010-154) (notice of filing and immediate effectiveness)....

  12. 76 FR 74103 - Self-Regulatory Organizations; National Stock Exchange, Inc.; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

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    ... No. 62252 (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS- 2010-014; SR-EDGA-2010-01... Exchange Act Release No. 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010-025). \\4\\ The... (September 10, 2010), 75 FR 56618 (September 16, 2010) (File Nos. SR-BATS-2010-018; SR-BX-2010-044;...

  13. Compress Your Files

    ERIC Educational Resources Information Center

    Branzburg, Jeffrey

    2005-01-01

    File compression enables data to be squeezed together, greatly reducing file size. Why would someone want to do this? Reducing file size enables the sending and receiving of files over the Internet more quickly, the ability to store more files on the hard drive, and the ability pack many related files into one archive (for example, all files…

  14. Hyperglycemia induces iNOS gene expression and consequent nitrosative stress via JNK activation

    PubMed Central

    YANG, Peixin; CAO, Yuanning; LI, Hua

    2010-01-01

    Objective Maternal diabetes adversely impacts embryonic development. We test the hypothesis that hyperglycemia-induced JNK1/2 activation mediates iNOS induction. Study Design Levels of iNOS mRNA and nitrosylated protein were determined in cultured C57BL/6J conceptuses exposed to hyperglycemia (300 mg/dl glucose) and C57BL/6J embryos exposed to streptozotocin-induced diabetes. iNOS-luciferase activity and endogenous reactive nitrogen species were determined in transfected PYS-2 (mouse teratocarcinoma) cells exposed to hyperglycemia (450 mg/dl glucose). Results Hyperglycemia increased iNOS mRNA and SP600125, a potent JNK1/2 inhibitor, abolished this effect. Hyperglycemia increased iNOS-luciferase activities and SP600125 blocked this effect. Diabetes increased iNOS mRNA and jnk2 gene deletion abrogated this effect. Correlated with iNOS gene induction, both hyperglycemia in vitro and diabetes in vivo enhanced the production of reactive nitrogen species and increased protein nitrosylation. jnk2 gene deletion blocked diabetes-induced protein nitrosylation. Conclusion JNK1/2 activation mediates hyperglycemia-induced iNOS gene expression and consequent nitrosative stress in diabetic embryopathy. PMID:20541731

  15. 76 FR 70418 - Marine Mammals; File No. 16124

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... National Oceanic and Atmospheric Administration RIN 0648-XA817 Marine Mammals; File No. 16124 AGENCY... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 16124 from the...@noaa.gov . Please include File No. 16124 in the subject line of the email comment. Those...

  16. 76 FR 18533 - Marine Mammals; File No. 15324

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-04

    ... National Oceanic and Atmospheric Administration RIN 0648-XA341 Marine Mammals; File No. 15324 AGENCY... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15324 from the... to (301) 713- 0376, or by e-mail to NMFS.Pr1Comments@noaa.gov . Please include the File No. in...

  17. 75 FR 39915 - Marine Mammals; File No. 15483

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-13

    ... National Oceanic and Atmospheric Administration RIN 0648-XX23 Marine Mammals; File No. 15483 AGENCY... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15483 from the... include the File No. in the subject line of the email comment. Those individuals requesting a...

  18. 76 FR 18533 - Marine Mammals; File No. 15682

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-04

    ... National Oceanic and Atmospheric Administration RIN 0648-XA342 Marine Mammals; File No. 15682 AGENCY... Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No... include the File No. in the subject line of the e-mail comment. Those individuals requesting a...

  19. Rapid NOS-1-derived nitric oxide and peroxynitrite formation act as signaling agents for inducible NOS-2 expression in vascular smooth muscle cells.

    PubMed

    Scheschowitsch, Karin; de Moraes, João Alfredo; Sordi, Regina; Barja-Fidalgo, Christina; Assreuy, Jamil

    2015-10-01

    Septic vascular dysfunction is characterized by hypotension and hyporeactivity to vasoconstrictors and nitric oxide (NO), reactive oxygen species and peroxynitrite have a prominent role in this condition. However, the mechanism whereby the vascular dysfunction is initiated is poorly understood. Based on previous studies of our group and the literature,we hypothesize that constitutive nitric oxide synthases (c-NOS) and peroxynitrite may play a role in the development of septic vascular dysfunction. Bacterial lipopolysaccharide (LPS) and interferon-γ (IFN) were used to stimulate rat aorta smooth muscle cells (A7r5) and rat aorta slices. This stimulation led to a rapid (within minutes) production of NO and superoxide anion, which led to peroxynitrite formation. When this rapid initial burst was reduced, through the inhibition of c-NOS and NADPH oxidases (NOX) or the scavenging of NO and superoxide the NF-κB activation, NOS-2 expression and nitrite production were significantly attenuated. Although vascular smooth muscle cells express both c-NOS isoforms, gene knockdown revealed that only NOS-1-dependent NO and peroxynitrite formation are important for the later NOS-2 expression. Similar findings were obtained by knockdown NOX-1 gene, one source of superoxide for peroxynitrite formation. Taking together, we show that smooth muscle cell activation by LPS/IFN leads to a rapid formation of NOS-1-derived NO and NOX-1-derived superoxide, forming peroxynitrite; and that this species act as a trigger for NOS-2 expression through NF-κB activation. Therefore, our findings suggest a critical role for NOS-1 and NOX-1 in the initiation of the vascular dysfunction associated with sepsis and septic shock. PMID:26253183

  20. 78 FR 57191 - Self-Regulatory Organizations; BOX Options Exchange LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-17

    ... restrictions. \\5\\ See Securities Exchange Act Release Nos. 66278 (January 30, 2012), 77 FR 5590 (February 3... on a pilot basis (SR-BX-2011-046), 66979 (May 14, 2012), 77 FR 29740 (May 18, 2012) (Notice of Filing...) (SR-BOX-2012-002), and 69054 (March 7, 2013), 78 FR 16025(March 13, 2013) (Notice of Filing...

  1. 78 FR 17741 - Self-Regulatory Organizations; BOX Options Exchange LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-22

    ... Release No. 68771 (January 30, 2013), 78 FR 8208 (February 5, 2013) (Notice of Filing and Immediate... Exchange Act Release Nos. 66871 (April 27, 2012) 77 FR 26323 (May 3, 2012) (File No.10- 206, In the Matter... Findings, Opinion, and Order of the Commission), and 67328 (June 29, 2012) 77 FR 40123 (July 6, 2012)...

  2. 78 FR 38416 - Self-Regulatory Organizations; Miami International Securities Exchange LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-26

    ... Release Nos. 67754 (August 29, 2012), 77 FR 54629 (September 5, 2012) (Order approving SR-ISE-2012- 33) (``ISE filing''); 69633 (May 23, 2012), 78 FR 32498 (May 30, 2013) (SR-Phlx-2013-55) (``PHLX filing''); 68074 (October 19, 2012), 77 FR 65241 (October 25, 2012) (SR-CBOE-2012-92); and 68193 (November 8,...

  3. 78 FR 75644 - Self-Regulatory Organizations; BOX Options Exchange LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-12

    ... Exchange Act Release Nos. 70472 (September 23, 2013), 78 FR 59738 (September 27, 2013)(Notice of Filing and Immediate Effectiveness of SR-PHLX-2013-93); 70470 (September 23, 2013) 78 FR 59740 (September 27, 2013)(Notice of Filing and Immediate Effectiveness of SR-NASDAQ-2013-117); and 70657 (October 10, 2013), 78...

  4. Reversal of SIN-1-induced eNOS dysfunction by the spin trap, DMPO, in bovine aortic endothelial cells via eNOS phosphorylation

    PubMed Central

    Das, Amlan; Gopalakrishnan, Bhavani; Druhan, Lawrence J; Wang, Tse-Yao; De Pascali, Francesco; Rockenbauer, Antal; Racoma, Ira; Varadharaj, Saradhadevi; Zweier, Jay L; Cardounel, Arturo J; Villamena, Frederick A

    2014-01-01

    Background and Purpose Nitric oxide (NO) derived from eNOS is mostly responsible for the maintenance of vascular homeostasis and its decreased bioavailability is characteristic of reactive oxygen species (ROS)-induced endothelial dysfunction (ED). Because 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), a commonly used spin trap, can control intracellular nitroso-redox balance by scavenging ROS and donating NO, it was employed as a cardioprotective agent against ED but the mechanism of its protection is still not clear. This study elucidated the mechanism of protection by DMPO against SIN-1-induced oxidative injury to bovine aortic endothelial cells (BAEC). Experimental Approach BAEC were treated with SIN-1, as a source of peroxynitrite anion (ONOO−), and then incubated with DMPO. Cytotoxicity following SIN-1 alone and cytoprotection by adding DMPO was assessed by MTT assay. Levels of ROS and NO generation from HEK293 cells transfected with wild-type and mutant eNOS cDNAs, tetrahydrobiopterin bioavailability, eNOS activity, eNOS and Akt kinase phosphorylation were measured. Key Results Post-treatment of cells with DMPO attenuated SIN-1-mediated cytotoxicity and ROS generation, restoration of NO levels via increased in eNOS activity and phospho-eNOS levels. Treatment with DMPO alone significantly increased NO levels and induced phosphorylation of eNOS Ser1179 via Akt kinase. Transfection studies with wild-type and mutant human eNOS confirmed the dual role of eNOS as a producer of superoxide anion (O2−) with SIN-1 treatment, and a producer of NO in the presence of DMPO. Conclusion and Implications Post-treatment with DMPO of oxidatively challenged cells reversed eNOS dysfunction and could have pharmacological implications in the treatment of cardiovascular diseases. PMID:24405159

  5. The return of the Scarlet Pimpernel: cobalamin in inflammation II - cobalamins can both selectively promote all three nitric oxide synthases (NOS), particularly iNOS and eNOS, and, as needed, selectively inhibit iNOS and nNOS.

    PubMed

    Wheatley, Carmen

    2007-09-01

    The up-regulation of transcobalamins [hitherto posited as indicating a central need for cobalamin (Cbl) in inflammation], whose expression, like inducible nitric oxide synthase (iNOS), is Sp1- and interferondependent, together with increased intracellular formation of glutathionylcobalamin (GSCbl), adenosylcobalamin (AdoCbl), methylcobalamin (MeCbl), may be essential for the timely promotion and later selective inhibition of iNOS and concordant regulation of endothelial and neuronal NOS (eNOS/nNOS.) Cbl may ensure controlled high output of nitric oxide (NO) and its safe deployment, because: (1) Cbl is ultimately responsible for the synthesis or availability of the NOS substrates and cofactors heme, arginine, BH(4) flavin adenine dinucleotide/flavin mononucleotide (FAD/FMN) and NADPH, via the far-reaching effects of the two Cbl coenzymes, methionine synthase (MS) and methylmalonyl CoA mutase (MCoAM) in, or on, the folate, glutathione, tricarboxylic acid (TCA) and urea cycles, oxidative phosphorylation, glycolysis and the pentose phosphate pathway. Deficiency of any of theNOS substrates and cofactors results in 'uncoupled' NOS reactions, decreasedNO production and increased or excessive O(2) (-), H(2)O(2), ONOO(-) and other reactive oxygen species (ROS), reactive nitric oxide species (RNIS) leading to pathology. (2) Cbl is also the overlooked ultimate determinant of positive glutathione status, which favours the formation of more benign NO species, s-nitrosothiols, the predominant form in which NO is safely deployed. Cbl status may consequently act as a 'back-up disc' that ensures the active status of antioxidant systems, as well as reversing and modulating the effects of nitrosylation in cell signal transduction.New evidence shows that GSCbl can significantly promote iNOS/ eNOS NO synthesis in the early stages of inflammation, thus lowering high levels of tumour necrosis factor-a that normally result in pathology, while existing evidence shows that in extreme

  6. The return of the Scarlet Pimpernel: cobalamin in inflammation II — cobalamins can both selectively promote all three nitric oxide synthases (NOS), particularly iNOS and eNOS, and, as needed, selectively inhibit iNOS and nNOS

    PubMed Central

    Wheatley, Carmen

    2007-01-01

    The up-regulation of transcobalamins [hitherto posited as indicating a central need for cobalamin (Cbl) in inflammation], whose expression, like inducible nitric oxide synthase (iNOS), is Sp1- and interferondependent, together with increased intracellular formation of glutathionylcobalamin (GSCbl), adenosylcobalamin (AdoCbl), methylcobalamin (MeCbl), may be essential for the timely promotion and later selective inhibition of iNOS and concordant regulation of endothelial and neuronal NOS (eNOS/nNOS.) Cbl may ensure controlled high output of nitric oxide (NO) and its safe deployment, because: (1) Cbl is ultimately responsible for the synthesis or availability of the NOS substrates and cofactors heme, arginine, BH4 flavin adenine dinucleotide/flavin mononucleotide (FAD/FMN) and NADPH, via the far-reaching effects of the two Cbl coenzymes, methionine synthase (MS) and methylmalonyl CoA mutase (MCoAM) in, or on, the folate, glutathione, tricarboxylic acid (TCA) and urea cycles, oxidative phosphorylation, glycolysis and the pentose phosphate pathway. Deficiency of any of theNOS substrates and cofactors results in ‘uncoupled’ NOS reactions, decreasedNO production and increased or excessive O2−, H2O2, ONOO− and other reactive oxygen species (ROS), reactive nitric oxide species (RNIS) leading to pathology. (2) Cbl is also the overlooked ultimate determinant of positive glutathione status, which favours the formation of more benign NO species, s-nitrosothiols, the predominant form in which NO is safely deployed. Cbl status may consequently act as a ‘back-up disc’ that ensures the active status of antioxidant systems, as well as reversing and modulating the effects of nitrosylation in cell signal transduction.New evidence shows that GSCbl can significantly promote iNOS/ eNOS NO synthesis in the early stages of inflammation, thus lowering high levels of tumour necrosis factor-a that normally result in pathology, while existing evidence shows that in extreme

  7. Nox2-dependent glutathionylation of endothelial NOS leads to uncoupled superoxide production and endothelial barrier dysfunction in acute lung injury

    PubMed Central

    Wu, Feng; Szczepaniak, William S.; Shiva, Sruti; Liu, Huanbo; Wang, Yinna; Wang, Ling; Wang, Ying; Kelley, Eric E.; Chen, Alex F.; Gladwin, Mark T.

    2014-01-01

    Microvascular barrier integrity is dependent on bioavailable nitric oxide (NO) produced locally by endothelial NO synthase (eNOS). Under conditions of limited substrate or cofactor availability or by enzymatic modification, eNOS may become uncoupled, producing superoxide in lieu of NO. This study was designed to investigate how eNOS-dependent superoxide production contributes to endothelial barrier dysfunction in inflammatory lung injury and its regulation. C57BL/6J mice were challenged with intratracheal LPS. Bronchoalveolar lavage fluid was analyzed for protein accumulation, and lung tissue homogenate was assayed for endothelial NOS content and function. Human lung microvascular endothelial cell (HLMVEC) monolayers were exposed to LPS in vitro, and barrier integrity and superoxide production were measured. Biopterin species were quantified, and coimmunoprecipitation (Co-IP) assays were performed to identify protein interactions with eNOS that putatively drive uncoupling. Mice exposed to LPS demonstrated eNOS-dependent increased alveolar permeability without evidence for altered canonical NO signaling. LPS-induced superoxide production and permeability in HLMVEC were inhibited by the NOS inhibitor nitro-l-arginine methyl ester, eNOS-targeted siRNA, the eNOS cofactor tetrahydrobiopterin, and superoxide dismutase. Co-IP indicated that LPS stimulated the association of eNOS with NADPH oxidase 2 (Nox2), which correlated with augmented eNOS S-glutathionylation both in vitro and in vivo. In vitro, Nox2-specific inhibition prevented LPS-induced eNOS modification and increases in both superoxide production and permeability. These data indicate that eNOS uncoupling contributes to superoxide production and barrier dysfunction in the lung microvasculature after exposure to LPS. Furthermore, the results implicate Nox2-mediated eNOS-S-glutathionylation as a mechanism underlying LPS-induced eNOS uncoupling in the lung microvasculature. PMID:25326583

  8. 75 FR 27335 - Combined Notice of Filings # 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-14

    ...: Portland General Electric Company. Description: Portland General Electric Company submits updated market... Corporation and BE KJ LLC's Supplement to Updated Market Power Analysis and Order Nos. 697 and 697-A... filing of qualification in ] the Forward Capacity Market for the 2013-2014 Capacity Commitment...

  9. 75 FR 70229 - Combined Notice of Filings No. 2

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ..., November 17, 2010. Docket Numbers: RP10-1037-001. Applicants: Granite State Gas Transmission, Inc. Description: Granite State Gas Transmission, Inc. Filing Addressing Compliance Obligations in Docket Nos. RP10..., 2010. Docket Numbers: RP10-963-002. Applicants: Granite State Gas Transmission, Inc....

  10. Case File: The Spazzoids

    MedlinePlus

    ... classroom activities. More Related Links Healthy Schools Case file: The Spazzzoids Recommend on Facebook Tweet Share Compartir ... your classroom activities. More Related Links Healthy Schools File Formats Help: How do I view different file ...

  11. 76 FR 51092 - Self-Regulatory Organizations; BATS Y-Exchange, Inc.; Notice of Filing of Proposed Rule Change To...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-17

    ... FR 34186 (June 16, 2010)(File Nos. SR-BATS-2010-014; SR-EDGA- 2010-01; SR-EDGX-2010-01; SR-BX-2010...- 061; SR-CHX-2010-10; SR-NSX-2010-05; and SR-CBOE-2010-047); 62251 (June 10, 2010), 75 FR 34183 (June... 10, 2010), 75 FR 56618 (September 16, 2010) (File Nos. SR-BATS-2010-018; SR-BX-2010-044;...

  12. 76 FR 27684 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing of a Proposed Rule Change To...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-12

    ... (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014; SR-EDGA-2010-01; SR-EDGX-2010... Release No. 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010-025). \\4\\ The Commission... (September 10, 2010), 75 FR 56618 (September 16, 2010) (File Nos. SR-BATS-2010-018; SR-BX-2010-044;...

  13. 76 FR 74099 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-30

    ...), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS- 2010-014; SR-EDGA-2010-01; SR-EDGX-2010-01; SR-BX.... 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010-025). \\5\\ The Commission approved the...), 75 FR 56618 (September 16, 2010) (File Nos. SR-BATS-2010-018; SR-BX-2010-044; SR-CBOE-2010-065;...

  14. 76 FR 74080 - Self-Regulatory Organizations; New York Stock Exchange LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-30

    ...), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS- 2010-014; SR-EDGA-2010-01; SR-EDGX-2010-01; SR-BX.... 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010-025). \\5\\ The Commission approved the...), 75 FR 56618 (September 16, 2010) (File Nos. SR-BATS-2010-018; SR-BX-2010-044; SR-CBOE-2010-065;...

  15. 76 FR 51105 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

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    2011-08-17

    ..., 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014; SR- EDGA-2010-01; SR-EDGX-2010-01; SR...- 2010-061; SR-CHX-2010-10; SR-NSX-2010-05; and SR-CBOE-2010-047) and 62251 (June 10, 2010), 75 FR 34183... (September 10, 2010), 75 FR 56618 (September 16, 2010) (File Nos. SR-BATS-2010-018; SR-BX-2010-044;...

  16. 43 CFR 4.1352 - Who may file; where to file; when to file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file; where to file; when to file... Indian Lands) § 4.1352 Who may file; where to file; when to file. (a) The applicant or operator may file... to file a timely request constitutes a waiver of the opportunity for a hearing before OSM makes...

  17. Common file formats.

    PubMed

    Leonard, Shonda A; Littlejohn, Timothy G; Baxevanis, Andreas D

    2007-01-01

    This appendix discusses a few of the file formats frequently encountered in bioinformatics. Specifically, it reviews the rules for generating FASTA files and provides guidance for interpreting NCBI descriptor lines, commonly found in FASTA files. In addition, it reviews the construction of GenBank, Phylip, MSF and Nexus files. PMID:18428774

  18. 77 FR 40126 - Self-Regulatory Organizations; NASDAQ OMX Phlx, LLC; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-06

    ... Release Nos. 55153 (January 23, 2007), 72 FR 4553 (January 31, 2007) (SR-Phlx-2006-74) (notice of filing and approval order establishing Penny Pilot); 60873 (October 23, 2009), 74 FR 56675 (November 2, 2009...); 60966 (November 9, 2009), 74 FR 59331 (November 17, 2009) (SR-Phlx-2009-94) (notice of filing...

  19. 75 FR 41560 - Self-Regulatory Organizations; New York Stock Exchange LLC and NYSE Amex LLC; Notice of Filing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION [Release No. 34-64277; File Nos. SR-NYSE-2010-48 and SR-NYSEAMEX-2010- 61] Self-Regulatory Organizations; New York Stock Exchange LLC and NYSE Amex LLC; Notice of Filing and Immediate Effectiveness of Proposed Rule Change Relating to...

  20. 76 FR 57776 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-16

    ..., 2011), 76 FR 1201 (January 7, 2011) (NASDAQ-2010-154) (notice of filing and immediate effectiveness). 2... the Investor Support Program, see Securities Exchange Act Release No. 63270 (November 8, 2010), 75 FR... Filing''). See also Securities Exchange Act Release Nos. 63414 (December 2, 2010), 75 FR 76505...

  1. 76 FR 74082 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-30

    ... Securities Exchange Act Release No. 62252 (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS...- 2010-047) and Securities Exchange Act Release No. 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010... Securities Exchange Act Release No. 62884 (September 10, 2010), 75 FR 56618 (September 16, 2010) (File...

  2. 76 FR 70784 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... as originally implemented, see Securities Exchange Act Release No. 63270 (November 8, 2010), 75 FR... Filing''). See also Securities Exchange Act Release Nos. 63414 (December 2, 2010), 75 FR 76505 (December...), 76 FR 1201 (January 7, 2011) (NASDAQ-2010-154) (notice of filing and immediate effectiveness);...

  3. 78 FR 17985 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ...), 78 FR 6382 (January 30, 2013) (SR-NASDAQ-2013-011) (notice of filing and immediate effectiveness of.... See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008)(SR-NASDAQ... 21, 2012), 78 FR 136 (January 2, 2013) (SR-NASDAQ-2012-143) (notice of filing and...

  4. 78 FR 16898 - Self-Regulatory Organizations; the NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-19

    ... COMMISSION Self-Regulatory Organizations; the NASDAQ Stock Market LLC; Notice of Filing and Immediate.... \\1\\ 15 U.S.C. 78s(b)(1). \\2\\ 17 CFR 240.19b-4. I. Self-Regulatory Organization's Statement of the... Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008) (SR-NASDAQ-2008-026) (notice of filing...

  5. 77 FR 39781 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing of Proposed Rule Change To...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... Market LLC (``Nasdaq'').\\8\\ \\8\\ See Securities Exchange Act Release Nos. 65709 (November 8, 2011), 76 FR 70795 (November 15, 2011) (File No. SR-NYSE-2011-38); 65710 (November 8, 2011), 76 FR 70790 (November 15, 2011) (File No. SR-NYSEAmex-2011-55); 65708 (November 8, 2011), 76 FR 70799 (November 15, 2011)...

  6. 75 FR 1442 - Self-Regulatory Organizations; Notice of Filing and Immediate Effectiveness of a Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-11

    ... 5, 2008. See Securities Exchange Act Release Nos. 48013 (June 11, 2003), 68 FR 35933 (June 17, 2003) (SR-Phlx-2002-55) (notice of filing and order approving); 49801 (June 3, 2004), 69 FR 32652 (June 10, 2004) (SR- Phlx-2004-38) (notice of filing and immediate effectiveness); 51768 (May 31, 2005), 70...

  7. Inducible nitric oxide synthase (iNOS) regulatory region variation in non-human primates

    PubMed Central

    Roodgar, Morteza; Ross, Cody T.; Kenyon, Nicholas J.; Marcelino, Gretchen; Smith, David Glenn

    2015-01-01

    Inducible nitric oxide synthase (iNOS) is an enzyme that plays a key role in intracellular immune response against respiratory infections. Since various species of nonhuman primates exhibit different levels of susceptibility to infectious respiratory diseases, and since variation in regulatory regions of genes is thought to play a key role in expression levels of genes, two candidate regulatory regions of iNOS were mapped, sequenced, and compared across five species of nonhuman primates: African green monkeys (chlorocebus sabeus), pig-tailed macaques (Macaca mulatta), cynomolgus macaques (Macaca fascicularis), Indian rhesus macaques (Macaca mulatta), and Chinese rhesus macaques (M. mulatta). In addition, we conducted an in silico analysis of the transcription factor binding sites associated with genetic variation in these two candidate regulatory regions across species. We found that only one of the two candidate regions showed strong evidence of involvement in iNOS regulation. Specifically, we found evidence of 13 conserved binding site candidates linked to iNOS regulation: AP-1, C/EBPB, CREB, GATA-1, GATA-3, NF-AT, NF-AT5, NF-κB, KLF4, Oct-1, PEA3, SMAD3, and TCF11. Additionally, we found evidence of interspecies variation in binding sites for several regulatory elements linked to iNOS (GATA-3, GATA-4, KLF6, SRF, STAT-1, STAT-3, OLF-1 and HIF-1) across species, especially in African green monkeys relative to other species. Given the key role of iNOS in respiratory immune response, the findings of this study might help guide the direction of future studies aimed to uncover the molecular mechanisms underlying the increased susceptibility of African green monkeys to several viral and bacterial respiratory infections. PMID:25675838

  8. Nitric Oxide Synthase Enzymes in the Airways of Mice Exposed to Ovalbumin: NOS2 Expression Is NOS3 Dependent

    PubMed Central

    Bratt, Jennifer M.; Williams, Keisha; Rabowsky, Michelle F.; Last, Michael S.; Franzi, Lisa M.; Last, Jerold A.; Kenyon, Nicholas J.

    2010-01-01

    Objectives and Design. The function of the airway nitric oxide synthase (NOS) isoforms and the lung cell types responsible for its production are not fully understood. We hypothesized that NO homeostasis in the airway is important to control inflammation, which requires upregulation, of NOS2 protein expression by an NOS3-dependent mechanism. Materials or Subjects. Mice from a C57BL/6 wild-type, NOS1−/−, NOS2−/−, and NOS3−/− genotypes were used. All mice strains were systemically sensitized and exposed to filtered air or ovalbumin (OVA) aerosol for two weeks to create a subchronic model of allergen-induced airway inflammation. Methods. We measured lung function, lung lavage inflammatory and airway epithelial goblet cell count, exhaled NO, nitrate and nitrite concentration, and airway NOS1, NOS2, and NOS3 protein content. Results. Deletion of NOS1 or NOS3 increases NOS2 protein present in the airway epithelium and smooth muscle of air-exposed animals. Exposure to allergen significantly reduced the expression of NOS2 protein in the airway epithelium and smooth muscle of the NOS3−/− strain only. This reduction in NOS2 expression was not due to the replacement of epithelial cells with goblet cells as remaining epithelial cells did not express NOS2. NOS1−/− animals had significantly reduced goblet cell metaplasia compared to C57Bl/6 wt, NOS2−/−, and NOS3−/− allergen-exposed mice. Conclusion. The airway epithelial and smooth muscle cells maintain a stable airway NO concentration under noninflammatory conditions. This “homeostatic” mechanism is unable to distinguish between NOS derived from the different constitutive NOS isoforms. NOS3 is essential for the expression of NOS2 under inflammatory conditions, while NOS1 expression contributes to allergen-induced goblet cell metaplasia. PMID:20953358

  9. WOLF: FITS file processor

    NASA Astrophysics Data System (ADS)

    Shamir, Lior

    2012-12-01

    WOLF processes FITS files and generates photometry files, annotated JPGs, opacity maps, background, transient detection and luminance changes detection. This software was used to process data for the Night Sky Live project.

  10. On the selectivity of neuronal NOS inhibitors

    PubMed Central

    Pigott, B; Bartus, K; Garthwaite, J

    2013-01-01

    Background and Purpose Isoform-selective inhibitors of NOS enzymes are desirable as research tools and for potential therapeutic purposes. Vinyl-l-N-5-(1-imino-3-butenyl)-l-ornithine (l-VNIO) and Nω-propyl-l-arginine (NPA) purportedly have good selectivity for neuronal over endothelial NOS under cell-free conditions, as does N-[(3-aminomethyl)benzyl]acetamidine (1400W), which is primarily an inducible NOS inhibitor. Although used in numerous investigations in vitro and in vivo, there have been surprisingly few tests of the potency and selectivity of these compounds in cells. This study addresses this deficiency and evaluates the activity of new and potentially better pyrrolidine-based compounds. Experimental Approach The inhibitors were evaluated by measuring their effect on NMDA-evoked cGMP accumulation in rodent hippocampal slices, a response dependent on neuronal NOS, and ACh-evoked cGMP synthesis in aortic rings of the same animals, an endothelial NOS-dependent phenomenon. Key Results l-VNIO, NPA and 1400W inhibited responses in both tissues but all showed less than fivefold higher potency in the hippocampus than in the aorta, implying useless selectivity for neuronal over endothelial NOS at the tissue level. In addition, the inhibitors had a 25-fold lower potency in the hippocampus than reported previously, the IC50 values being approximately 1 μM for l-VNIO and NPA, and 150 μM for 1400W. Pyrrolidine-based inhibitors were similarly weak and nonselective. Conclusion and Implications The results suggest that l-VNIO, NPA and 1400W, as well as the newer pyrrolidine-type inhibitors, cannot be used as neuronal NOS inhibitors in cells without stringent verification. The identification of inhibitors with useable selectivity in cells and tissues remains an important goal. PMID:23072468

  11. Standard interface file handbook

    SciTech Connect

    Shapiro, A.; Huria, H.C. )

    1992-10-01

    This handbook documents many of the standard interface file formats that have been adopted by the US Department of Energy to facilitate communications between and portability of, various large reactor physics and radiation transport software packages. The emphasis is on those files needed for use of the VENTURE/PC diffusion-depletion code system. File structures, contents and some practical advice on use of the various files are provided.

  12. Text File Display Program

    NASA Technical Reports Server (NTRS)

    Vavrus, J. L.

    1986-01-01

    LOOK program permits user to examine text file in pseudorandom access manner. Program provides user with way of rapidly examining contents of ASCII text file. LOOK opens text file for input only and accesses it in blockwise fashion. Handles text formatting and displays text lines on screen. User moves forward or backward in file by any number of lines or blocks. Provides ability to "scroll" text at various speeds in forward or backward directions.

  13. Disrupted NOS signaling in lymphatic endothelial cells exposed to chronically increased pulmonary lymph flow.

    PubMed

    Datar, Sanjeev A; Gong, Wenhui; He, Youping; Johengen, Michael; Kameny, Rebecca J; Raff, Gary W; Maltepe, Emin; Oishi, Peter E; Fineman, Jeffrey R

    2016-07-01

    Associated abnormalities of the lymphatic circulation are well described in congenital heart disease. However, their mechanisms remain poorly elucidated. Using a clinically relevant ovine model of a congenital cardiac defect with chronically increased pulmonary blood flow (shunt), we previously demonstrated that exposure to chronically elevated pulmonary lymph flow is associated with: 1) decreased bioavailable nitric oxide (NO) in pulmonary lymph; and 2) attenuated endothelium-dependent relaxation of thoracic duct rings, suggesting disrupted lymphatic endothelial NO signaling in shunt lambs. To further elucidate the mechanisms responsible for this altered NO signaling, primary lymphatic endothelial cells (LECs) were isolated from the efferent lymphatic of the caudal mediastinal node in 4-wk-old control and shunt lambs. We found that shunt LECs (n = 3) had decreased bioavailable NO and decreased endothelial nitric oxide synthase (eNOS) mRNA and protein expression compared with control LECs (n = 3). eNOS activity was also low in shunt LECs, but, interestingly, inducible nitric oxide synthase (iNOS) expression and activity were increased in shunt LECs, as were total cellular nitration, including eNOS-specific nitration, and accumulation of reactive oxygen species (ROS). Pharmacological inhibition of iNOS reduced ROS in shunt LECs to levels measured in control LECs. These data support the conclusion that NOS signaling is disrupted in the lymphatic endothelium of lambs exposed to chronically increased pulmonary blood and lymph flow and may contribute to decreased pulmonary lymphatic bioavailable NO. PMID:27199125

  14. The Complex Role of iNOS in Acutely-Rejecting Cardiac Transplants

    PubMed Central

    Pieper, Galen M.; Roza, Allan M.

    2008-01-01

    This review summarizes the evidence for a detrimental role of nitric oxide (NO) derived from inducible NO synthase (iNOS) and/or reactive nitrogen species such as peroxynitrite in acutely-rejecting cardiac transplants. In chronic cardiac transplant rejection, iNOS may have an opposing beneficial component. The purpose of this review is primarily to address issues related to acute rejection which is a recognized risk factor for chronic rejection. The evidence for a detrimental role is based upon strategies involving non-selective NOS inhibitors, NO neutralizers, selective iNOS inhibitors and iNOS gene deletion in rodent models of cardiac rejection. The review is discussed in the context of the impact on various components including graft survival, histological rejection and cardiac function which may contribute in toto to the process of graft rejection. Possible limitations of each strategy are discussed in order to understand better the variance in published findings including issues related to the potential importance of cell localization of iNOS expression. Finally, the concept of a dual role of NO and its down-stream product, peroxynitrite, in rejection vs. immune regulation is discussed. PMID:18291116

  15. Text File Comparator

    NASA Technical Reports Server (NTRS)

    Kotler, R. S.

    1983-01-01

    File Comparator program IFCOMP, is text file comparator for IBM OS/VScompatable systems. IFCOMP accepts as input two text files and produces listing of differences in pseudo-update form. IFCOMP is very useful in monitoring changes made to software at the source code level.

  16. Multifaceted NOS Instruction: Contextualizing Nature of Science with Documentary Films

    ERIC Educational Resources Information Center

    Bloom, Mark; Binns, Ian C.; Koehler, Catherine

    2015-01-01

    This research focuses on inservice science teachers' conceptions of nature of science (NOS) before and after a two-week intensive summer professional development (PD). The PD combined traditional explicit NOS instruction, numerous interactive interventions that highlighted NOS aspects, along with documentary films that portrayed NOS in context of…

  17. 78 FR 15933 - Marine Mammals; File No. 17952

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-13

    ... National Oceanic and Atmospheric Administration RIN 0648-XC554 Marine Mammals; File No. 17952 AGENCY... Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 17952 from the list of... submitted by facsimile to (301) 713-0376, or by email to NMFS.Pr1Comments@noaa.gov . Please include the...

  18. 77 FR 36999 - Marine Mammals; File No. 16160

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-20

    ... (77 FR 35657), authorizes takes of eight species of cetaceans in the inland waters of Washington State... National Oceanic and Atmospheric Administration RIN 0648-XA626 Marine Mammals; File No. 16160 AGENCY....nmfs.noaa.gov , and then selecting File No. 16160 from the list of available applications....

  19. Carbon monoxide increases inducible NOS expression that mediates CO-induced myocardial damage during ischemia-reperfusion.

    PubMed

    Meyer, Grégory; André, Lucas; Kleindienst, Adrien; Singh, François; Tanguy, Stéphane; Richard, Sylvain; Obert, Philippe; Boucher, François; Jover, Bernard; Cazorla, Olivier; Reboul, Cyril

    2015-04-01

    We investigated the role of inducible nitric oxide (NO) synthase (iNOS) on ischemic myocardial damage in rats exposed to daily low nontoxic levels of carbon monoxide (CO). CO is a ubiquitous environmental pollutant that impacts on mortality and morbidity from cardiovascular diseases. We have previously shown that CO exposure aggravates myocardial ischemia-reperfusion (I/R) injury partly because of increased oxidative stress. Nevertheless, cellular mechanisms underlying cardiac CO toxicity remain hypothetical. Wistar rats were exposed to simulated urban CO pollution for 4 wk. First, the effects of CO exposure on NO production and NO synthase (NOS) expression were evaluated. Myocardial I/R was performed on isolated perfused hearts in the presence or absence of S-methyl-isothiourea (1 μM), a NOS inhibitor highly specific for iNOS. Finally, Ca(2+) handling was evaluated in isolated myocytes before and after an anoxia-reoxygenation performed with or without S-methyl-isothiourea or N-acetylcystein (20 μM), a nonspecific antioxidant. Our main results revealed that 1) CO exposure altered the pattern of NOS expression, which is characterized by increased neuronal NOS and iNOS expression; 2) cardiac NO production increased in CO rats because of its overexpression of iNOS; and 3) the use of a specific inhibitor of iNOS reduced myocardial hypersensitivity to I/R (infarct size, 29 vs. 51% of risk zone) in CO rat hearts. These last results are explained by the deleterious effects of NO and reactive oxygen species overproduction by iNOS on diastolic Ca(2+) overload and myofilaments Ca(2+) sensitivity. In conclusion, this study highlights the involvement of iNOS overexpression in the pathogenesis of simulated urban CO air pollution exposure. PMID:25595132

  20. 43 CFR 4.1381 - Who may file; when to file; where to file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... may file; when to file; where to file. (a) Any person who receives a written decision issued by OSM under 30 CFR 773.28 on a challenge to an ownership or control listing or finding may file a request for... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file; when to file; where to...

  1. Nitrous oxide reductase (nosZ) gene fragments differ between native and cultivated Michigan soils.

    PubMed

    Stres, Blaz; Mahne, Ivan; Avgustin, Gorazd; Tiedje, James M

    2004-01-01

    The effect of standard agricultural management on the genetic heterogeneity of nitrous oxide reductase (nosZ) fragments from denitrifying prokaryotes in native and cultivated soil was explored. Thirty-six soil cores were composited from each of the two soil management conditions. nosZ gene fragments were amplified from triplicate samples, and PCR products were cloned and screened by restriction fragment length polymorphism (RFLP). The total nosZ RFLP profiles increased in similarity with soil sample size until triplicate 3-g samples produced visually identical RFLP profiles for each treatment. Large differences in total nosZ profiles were observed between the native and cultivated soils. The fragments representing major groups of clones encountered at least twice and four randomly selected clones with unique RFLP patterns were sequenced to verify nosZ identity. The sequence diversity of nosZ clones from the cultivated field was higher, and only eight patterns were found in clone libraries from both soils among the 182 distinct nosZ RFLP patterns identified from the two soils. A group of clones that comprised 32% of all clones dominated the gene library of native soil, whereas many minor groups were observed in the gene library of cultivated soil. The 95% confidence intervals of the Chao1 nonparametric richness estimator for nosZ RFLP data did not overlap, indicating that the levels of species richness are significantly different in the two soils, the cultivated soil having higher diversity. Phylogenetic analysis of deduced amino acid sequences grouped the majority of nosZ clones into an interleaved Michigan soil cluster whose cultured members are alpha-Proteobacteria. Only four nosZ sequences from cultivated soil and one from the native soil were related to sequences found in gamma-Proteobacteria. Sequences from the native field formed a distinct, closely related cluster (D(mean) = 0.16) containing 91.6% of the native clones. Clones from the cultivated field were

  2. 21 CFR 516.163 - Change in ownership of an index file.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 6 2011-04-01 2011-04-01 false Change in ownership of an index file. 516.163... (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS NEW ANIMAL DRUGS FOR MINOR USE AND MINOR SPECIES Index... index file. (a) A holder may transfer ownership of a drug's index file to another person. (1) The...

  3. 21 CFR 516.163 - Change in ownership of an index file.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 6 2014-04-01 2014-04-01 false Change in ownership of an index file. 516.163... (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS NEW ANIMAL DRUGS FOR MINOR USE AND MINOR SPECIES Index... index file. (a) A holder may transfer ownership of a drug's index file to another person. (1) The...

  4. 21 CFR 516.163 - Change in ownership of an index file.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 6 2012-04-01 2012-04-01 false Change in ownership of an index file. 516.163... (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS NEW ANIMAL DRUGS FOR MINOR USE AND MINOR SPECIES Index... index file. (a) A holder may transfer ownership of a drug's index file to another person. (1) The...

  5. 21 CFR 516.163 - Change in ownership of an index file.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Change in ownership of an index file. 516.163... (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS NEW ANIMAL DRUGS FOR MINOR USE AND MINOR SPECIES Index... index file. (a) A holder may transfer ownership of a drug's index file to another person. (1) The...

  6. Catalytic Promiscuity of the Radical S-adenosyl-L-methionine Enzyme NosL

    PubMed Central

    Ding, Wei; Ji, Xinjian; Li, Yongzhen; Zhang, Qi

    2016-01-01

    Catalytic promiscuity plays a key role in enzyme evolution and the acquisition of novel biological functions. Because of the high reactivity of radical species, in our view enzymes involving radical-mediated mechanisms could intrinsically be more prone to catalytic promiscuity. This mini-review summarizes the recent advances in the study of NosL, a radical S-adenosyl-L-methionine (SAM)-dependent L-tryptophan (L-Trp) lyase. We demonstrate here the interesting chemistry and remarkable catalytic promiscuity of NosL, and attempt to highlight the high evolvability of radical SAM enzymes and the potential to engineer these enzymes for novel and improved activities. PMID:27446906

  7. EPA FACILITY POINT LOCATION FILES

    EPA Science Inventory

    Data includes locations of facilities from which pollutants are discharged. The epapoints.tar.gz file is a gzipped tar file of 14 Arc/Info export files and text documents. The .txt files define the attributes located in the INFO point coverage files. Projections are defined in...

  8. Medical image file formats.

    PubMed

    Larobina, Michele; Murino, Loredana

    2014-04-01

    Image file format is often a confusing aspect for someone wishing to process medical images. This article presents a demystifying overview of the major file formats currently used in medical imaging: Analyze, Neuroimaging Informatics Technology Initiative (Nifti), Minc, and Digital Imaging and Communications in Medicine (Dicom). Concepts common to all file formats, such as pixel depth, photometric interpretation, metadata, and pixel data, are first presented. Then, the characteristics and strengths of the various formats are discussed. The review concludes with some predictive considerations about the future trends in medical image file formats. PMID:24338090

  9. An evolutionarily ancient NO synthase (NOS) in shrimp.

    PubMed

    Wu, Chun-Hung; Siva, Vinu S; Song, Yen-Ling

    2013-11-01

    Nitric oxide (NO) is a well known essential molecule that is involved in multiple functions such as neuron transduction, cardiac disease, immune responses, etc.; nitric oxide synthase (NOS) is a critical enzyme that catalyzes the synthesis of it. A very few crustacean NOS molecules were biochemically characterized so far. In the present study, we cloned and characterized a NOS cDNA from haemocytes of tiger shrimp (Penaeus monodon) (PmNOS). The full-length of PmNOS cDNA contained 3997 bp, including a 5'UTR of 249 bp, ORF of 3582 bp and a 3'UTR of 166 bp. The putative peptide was 1193 amino acid residues in length, with an estimated molecular weight of 134.7 kDa and pI 6.7. Structurally, PmNOS contained oxygenase and reductase domains at N-terminal and C-terminal, respectively, and connected with a calmodulin binding motif. The deduced amino acid sequence of PmNOS shared 98% identical to the Chinese shrimp (Fenneropenaeus chinensis) NOS. Phylogenetically, PmNOS clustered with invertebrate NOS, but not clustered with iNOS, eNOS or nNOS found in vertebrates. PmNOS mRNA was expressed in many tissues or organs including thoracic and ventral nerves, midgut, gill, eyestalk, haemocytes, subcuticular epithelium and heart, but not found in hepatopancreas, muscle and lymphoid organ. But there was no significant difference in PmNOS mRNA expression after stimulation with LPS either by different concentration or time course or against CpG-ODN 2006. The enzyme activities of rPmNOS or crude homogenates from different tissues were detected, and were shown its highest activity in thoracic and ventral nerves, moderate in midgut and haemocytes but the lowest activity were seen in muscle. The addition of NOS antibody against NADPH binding domain leads to less activity which suggested that NADPH was an essential cofactor for PmNOS catalytic activity. The calcium dependency of PmNOS was ascertained using calmodulin inhibitor, Trifluroperazine. To confirm the population of haemocyte which

  10. 75 FR 65692 - Self-Regulatory Organizations; Notice of Filing and Immediate Effectiveness of Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-26

    ... Release Nos. 60694 (September 18, 2009), 74 FR 49048 (September 25, 2009) (SR-Phlx-2009-65) (order approving); and 61630 (March 2, 2010), 75 FR 11211 (March 10, 2010) (SR-Phlx-2010-26) (notice of filing and... Exchange added $5 to the listed strike prices of $2, $3 and $4 to account for the overlap with the...

  11. 77 FR 19400 - Self-Regulatory Organizations; EDGX Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ... Release No. 65565 (October 14, 2011), 76 FR 65255 (October 20, 2011) (SR-EDGX-2011-33). \\5\\ See Securities Exchange Act Release No. 66171 (January 17, 2012), 77 FR 3297 (January 23, 2012) (File Nos. SR-EDGA-2011-34... NYSE Euronext and Deutsche B rse AG Will Not Become Effective March 26, 2012 Pursuant to Section...

  12. 77 FR 19391 - Self-Regulatory Organizations; EDGA Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ... Release No. 65564 (October 14, 2011), 76 FR 65264 (October 20, 2011) (SR-EDGA-2011-34). \\5\\ See Securities Exchange Act Release No. 66171 (January 17, 2012), 77 FR 3297 (January 23, 2012) (File Nos. SR-EDGA-2011-34... NYSE Euronext and Deutsche B rse AG Will Not Become Effective March 26, 2012. Pursuant to Section...

  13. 77 FR 15417 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-15

    .... 56955 (December 13, 2007); 72 FR 71979 (December 19, 2007) (SR-ISE-2007-101). On December 23, 2008, the... (December 22, 2008); 73 FR 79954 (December 30, 2008) (SR-ISE-2008-85). On May 7, 2009, Direct Edge's direct... Release No. 60651 (September 11, 2009); 74 FR 47827 (September 17, 2009) (File Nos. 10-193 and 10-194)....

  14. 76 FR 44388 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ...), 75 FR 47335 (August 5, 2010) (SR-BATS-2010-020) (notice of filing and immediate effectiveness...), 76 FR 8793 (February 15, 2011) (SR-Phlx-2010-183) (order granting approval of expansion of short term... Exchange Act Release Nos. 64009 (March 2, 2011), 76 FR 12771 (March 8, 2011) (SR-BX-2011-014) (notice...

  15. 76 FR 1208 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-07

    ... Release Nos. 62663 (August 9, 2010), 75 FR 49543 (August 13, 2010) (SR-NASDAQ-2010-077) (order approving... (August 10, 2010), 75 FR 50020 (August 16, 2010) (SR-EDGA-2010-06) (order approving fees for both 1G and... COMMISSION Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing and Immediate...

  16. 76 FR 39463 - Self-Regulatory Organizations; National Securities Clearing Corporation; Notice of Filing of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... information on the Obligation Warehouse service, see Exchange Act Release 63588 (December 21, 2010), 75 FR.... SR-NSCC-2010-01], 75 FR 10542 (March 8, 2010). \\6\\ In order to distinguish securities transfers from... NSCC, see Exchange Act Release No. 40179 (July 8, 1998), 63 FR 38221 (July 15, 1998) (File Nos....

  17. 78 FR 76691 - Self-Regulatory Organizations; BOX Options Exchange LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-18

    ... Securities Exchange Findings, Opinion, and Order of the Commission), 67328 (June 29, 2012) 77 FR 40123 (July...-021) and 69789 (June 18, 2013) 78 FR 37854 (June 24, 2013) (SR-BOX-2013-31). The extension of the.... See Securities Exchange Act Release Nos. 66871 (April 27, 2012) 77 FR 26323 (May 3, 2012) (File...

  18. 75 FR 36458 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-25

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and Immediate Effectiveness of Proposed Rule Change, as Modified by Amendment Nos. 1 and 2, Related to the Price Improvement Mechanism June 17,...

  19. 78 FR 54294 - Investment Company Act Release No. 30679; File No. 812-14167

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-03

    ... COMMISSION Investment Company Act Release No. 30679; File No. 812-14167 Franklin Templeton International... (``Commission''). ACTION: Notice of an application under section 6(c) of the Investment Company Act of 1940..., Investment Company Act Release Nos. 30105 (Jun. 18, 2012) (notice) and 30138 (Jul. 17, 2012)...

  20. 78 FR 28692 - Self-Regulatory Organizations; BOX Options Exchange LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-15

    ... Securities Exchange Act Release No. 69511 (May 03, 2013), 78 FR 27271 (May 9, 2013) (Order Approving SR-BOX-2013-06). \\5\\ Id. \\6\\ Id. \\7\\ See Securities Exchange Act Release Nos. 69154 (March 15, 2013), 78 FR..., 2013), 78 FR 19562 (April 1, 2013) (Notice of Filing and Immediate Effectiveness of SR-BOX-2013-18)...

  1. 75 FR 65693 - Self-Regulatory Organizations; Notice of Filing and Immediate Effectiveness of Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-26

    ... Act Release Nos. 59277 (November 6, 2009), 74 FR 59277 (November 17, 2009 (SR-NASDAQ-2009-099) (order approving); and 61736 (March 18, 2010), 75 FR 14229 (March 24, 2010) (SR-NASDAQ-2010-038) (notice of filing... Change by the NASDAQ Stock Market, LLC To Expand the $0.50 Strike Price Program Date: October 20,...

  2. 77 FR 61036 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-05

    ... COMMISSION Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and... intermarket linkage rules.\\8\\ \\6\\ See Securities Exchange Act Release Nos. 57812 (May 12, 2008), 73 FR 28846 (May 19, 2008) (SR-ISE-2008-50); 58038 (June 26, 2008), 73 FR 38261 (June July 3, 2008)...

  3. 76 FR 22425 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-21

    ... COMMISSION Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and... Exchange's Schedule of Fees. See Securities Exchange Act Release Nos. 61869 (April 7, 2010), 75 FR 19449 (April 14, 2010) (SR-ISE-2010-25), 62048 (May 6, 2010), 75 FR 26830 (May 12, 2010)...

  4. 77 FR 35723 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... COMMISSION Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and... and proposes to eliminate it. \\3\\ See Exchange Act Release Nos. 34-54751 (November 14, 2006), 71 FR 67667 (November 22, 2006) (SR-ISE-2006-56); 55247 (February 6, 2007), 72 FR 7099 (February 14, 2007)...

  5. 76 FR 50805 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-16

    ... COMMISSION Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and... Fees. See Securities Exchange Act Release Nos. 61869 (April 7, 2010), 75 FR 19449 (April 14, 2010) (SR-ISE-2010-25), 62048 (May 6, 2010), 75 FR 26830 (May 12, 2010) (SR-ISE-2010-43), 62282 (June 11,...

  6. 76 FR 19160 - Self-Regulatory Organizations; Notice of Filing and Immediate Effectiveness of Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    .... \\3\\ See Securities Exchange Act Release No. 35993 (July 19, 1995), 60 FR 38073 (July 25, 1995... also Exchange Act Release No. 52893 (December 5, 2005) 70 FR 73488 (December 12, 2005) (a rule change... Exchange Act Release No. 40662 (November 12, 1998), 63 FR 64297 (November 19, 1998) (approving File Nos....

  7. 76 FR 19165 - Self-Regulatory Organizations; Notice of Filing and Immediate Effectiveness of Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    .... \\3\\ See Securities Exchange Act Release No. 35993 (July 19, 1995), 60 FR 38073 (July 25, 1995... Exchange Act Release No. 52986 (December 20, 2005) 70 FR 76897 (December 28, 2005) (a rule change to allow... Exchange Act Release No. 40662 (November 12, 1998), 63 FR 64297 (November 19, 1998) (approving File Nos....

  8. 76 FR 20764 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... option classes. See Securities Exchange Act Release No. 35993 (July 19, 1995), 60 FR 38073 (July 25, 1995... (November 12, 1998), 63 FR 64297 (November 19, 1998) (approving File Nos. SR-Amex- 98-21, SR-CBOE-98-29, SR... Strike Price Program. See Securities Exchange Act Release No. 52960 (December 15, 2005), 70 FR...

  9. 75 FR 52790 - Self-Regulatory Organizations; NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-27

    ... through December 31, 2010. See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587... Pilot); 60874 (October 23, 2009), 74 FR 56682 (November 2, 2009) (SR-NASDAQ-2009-091) (notice of filing and immediate effectiveness expanding and extending Penny Pilot); 60965 (November 9, 2009), 74...

  10. 75 FR 71475 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-23

    ....\\8\\ \\8\\ Securities Exchange Act Release No. 51808 (June 9, 2005), 70 FR 37496 (June 29, 2005). By... Securities Exchange Act Release Nos. 63084 (October 13, 2010), 75 FR 64379 (October 19, 2010) (Notice of... (September 10, 2010), 75 FR 57092 (September 17, 2010) (Notice of Filing and Immediate Effectiveness...

  11. 76 FR 76472 - Self-Regulatory Organizations; NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-07

    ... Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008) (SR-NASDAQ-2008-026) (notice of filing and immediate effectiveness establishing Penny Pilot); 60874 (October 23, 2009), 74 FR... and extending Penny Pilot); 60965 (November 9, 2009), 74 FR 59292 (November 17, 2009)...

  12. 77 FR 2335 - Self-Regulatory Organizations; NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-17

    ... Release No. 61358 (January 14, 2010), 75 FR 3594 (January 21, 2010) (Concept Release on Equity Market... December 31, 2011. See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4... (October 23, 2009), 74 FR 56682 (November 2, 2009)(SR-NASDAQ-2009-091)(notice of filing and...

  13. 77 FR 65241 - Self-Regulatory Organizations; Chicago Board Options Exchange, Incorporated; Notice of Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-25

    ... non-Weekly options expire. \\6\\ See Securities Exchange Act Release Nos. 67754 (August 29, 2012), 77 FR...) 77 FR 54635 (September 5, 2012) (order approving SR-Phlx-2012-78) (``Phlx filing''). ISE and Phlx... Exchange is making a distinction between initiating series and cloning series. The Exchange and...

  14. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... file electronically under 11 CFR 104.18, may file 24-hour reports using the Commission's website's on... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or... required by 11 CFR part 105, by the close of business on the prescribed filing date. (b) Timely filed....

  15. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... file electronically under 11 CFR 104.18, may file 24-hour reports using the Commission's website's on... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or... required by 11 CFR part 105, by the close of business on the prescribed filing date. (b) Timely filed....

  16. 75 FR 30017 - Electronic Tariff Filings; Notice of Posting Regarding Filing Procedures for Electronically Filed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-28

    ... Energy Regulatory Commission Electronic Tariff Filings; Notice of Posting Regarding Filing Procedures for Electronically Filed Tariffs May 21, 2010. Take Notice that the attached document ``Filing Procedures For Electronically Filed Tariffs, Rate Schedules And Jurisdictional Agreements'' has been posted on the eTariff...

  17. 15 CFR Supplement Nos. 3-4 to Part... - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Nos. Supplement Nos. 3-4 to Part 742 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF... CONTROLS Supplement Nos. 3-4 to Part 742...

  18. Hopper File Management Tool

    SciTech Connect

    Long, J W; O'Neill, N J; Smith, N G; Springmeyer, R R; Remmele, S; Richards, D A; Southon, J

    2004-11-15

    Hopper is a powerful interactive tool that allows users to transfer and manipulate files and directories by means of a graphical user interface. Users can connect to and manage resources using the major file transfer protocols. Implemented in Java, Hopper can be run almost anywhere: from an individual's desktop machine to large production machines. In a high-performance computing environment, managing files can become a difficult and time-consuming task that distracts from scientific work. Users must deal with multiple file transfer protocols, transferring enormous amounts of files between computer platforms, repeated authentication, organizing massive amounts of data, and other detailed but necessary tasks. This is often accomplished with a set of several different tools, each with its own interface and idiosyncrasies. Our goal is to develop tools for a more automated approach to file management that substantially improves users' ability to transfer, organize, search, and operate on collections of files. This paper describes the Hopper tool for advanced file management, including the software architecture, the functionality, and the user interface.

  19. NASA Uniform Files Index

    NASA Technical Reports Server (NTRS)

    1987-01-01

    This handbook is a guide for the use of all personnel engaged in handling NASA files. It is issued in accordance with the regulations of the National Archives and Records Administration, in the Code of Federal Regulations Title 36, Part 1224, Files Management; and the Federal Information Resources Management Regulation, Subpart 201-45.108, Files Management. It is intended to provide a standardized classification and filing scheme to achieve maximum uniformity and ease in maintaining and using agency records. It is a framework for consistent organization of information in an arrangement that will be useful to current and future researchers. The NASA Uniform Files Index coding structure is composed of the subject classification table used for NASA management directives and the subject groups in the NASA scientific and technical information system. It is designed to correlate files throughout NASA and it is anticipated that it may be useful with automated filing systems. It is expected that in the conversion of current files to this arrangement it will be necessary to add tertiary subjects and make further subdivisions under the existing categories. Established primary and secondary subject categories may not be changed arbitrarily. Proposals for additional subject categories of NASA-wide applicability, and suggestions for improvement in this handbook, should be addressed to the Records Program Manager at the pertinent installation who will forward it to the NASA Records Management Office, Code NTR, for approval. This handbook is issued in loose-leaf form and will be revised by page changes.

  20. JENDL Dosimetry File 99.

    Energy Science and Technology Software Center (ESTSC)

    2001-01-22

    Version 00 JENDL/D-99 contains information for 47 nuclides and 67 reactions in the SAND-II group structure (although it was observed by RSICC that not all of the processed files are in the SAND-II group structure) and as 0K preprocessed pointwise files.

  1. 21 CFR 516.147 - Refuse to file a request for addition to the index.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 6 2014-04-01 2014-04-01 false Refuse to file a request for addition to the index... SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.147 Refuse to file a request for addition to the index. (a) If a request for addition to the index contains all of...

  2. Register file soft error recovery

    DOEpatents

    Fleischer, Bruce M.; Fox, Thomas W.; Wait, Charles D.; Muff, Adam J.; Watson, III, Alfred T.

    2013-10-15

    Register file soft error recovery including a system that includes a first register file and a second register file that mirrors the first register file. The system also includes an arithmetic pipeline for receiving data read from the first register file, and error detection circuitry to detect whether the data read from the first register file includes corrupted data. The system further includes error recovery circuitry to insert an error recovery instruction into the arithmetic pipeline in response to detecting the corrupted data. The inserted error recovery instruction replaces the corrupted data in the first register file with a copy of the data from the second register file.

  3. NOS knockout or inhibition but not disrupting PSD-95-NOS interaction protect against ischemic brain damage.

    PubMed

    Kleinschnitz, Christoph; Mencl, Stine; Kleikers, Pamela Wm; Schuhmann, Michael K; G López, Manuela; Casas, Ana I; Sürün, Bilge; Reif, Andreas; Schmidt, Harald Hhw

    2016-09-01

    Promising results have been reported in preclinical stroke target validation for pharmacological principles that disrupt the N-methyl-D-aspartate receptor-post-synaptic density protein-95-neuronal nitric oxide synthase complex. However, post-synaptic density protein-95 is also coupled to potentially neuroprotective mechanisms. As post-synaptic density protein-95 inhibitors may interfere with potentially neuroprotective mechanisms and sufficient validation has often been an issue in translating basic stroke research, we wanted to close that gap by comparing post-synaptic density protein-95 inhibitors with NOS1(-/-) mice and a NOS inhibitor. We confirm the deleterious role of NOS1 in stroke both in vivo and in vitro, but find three pharmacological post-synaptic density protein-95 inhibitors to be therapeutically ineffective. PMID:27354091

  4. Registered File Support for Critical Operations Files at SIRTF

    NASA Astrophysics Data System (ADS)

    Turek, G.; Handley, T.; Jacobson, J.; Rector, J.

    The SIRTF Science Center's (SSC) Science Operations System (SOS) has to contend with nearly one hundred critical operations files via comprehensive file management services. The management is accomplished via the registered file system (otherwise known as TFS) which manages these files in a registered file repository composed of a virtual file system accessible via a TFS server and a file registration database. The TFS server provides controlled, reliable, and secure file transfer and storage by registering all file transactions and meta-data in the file registration database. An API is provided for application programs to communicate with TFS servers and the repository. A command line client implementing this API has been developed as a client tool. This paper describes the architecture, current implementation, and more importantly, the evolution of these services based on evolving community use cases and emerging information system technology.

  5. SS focused technology: Gateways and NOS's

    NASA Technical Reports Server (NTRS)

    Hartenstein, R.

    1985-01-01

    The extensions and enhancements of the fiber optic data bus technology supported by the Space Station Focused Technology Program are discussed. This includes the operating software for the network called the Network Operating System (NOS); gateways and bridges for multiple network topologies; and very large scale topology implimentations to shrink the size and power of the Bus Interface Unit (BIU) down to more manageable dimensions. CMOS is being investigated for the lower speed (parallel) logic. Gallium arsendide is being studied for the high speed (serial) logic.

  6. MAWST file generator (MFG)

    SciTech Connect

    Henriksen, P.W.; Hurdle, S.; Hafer, J.F.

    1993-12-01

    The software program MAWST was developed as a tool to deal with common materials accounting problems. The key to successful usage of this program is in the generation of input files for measurement values, measurement errors, and measurement methods. The program MFG was developed as an aid to creating input files for MAWST. MFG contains three commonly used measurements -- nondestructive assay, (G-T)*C, and V*C -- and a GENERIC measurement as models for data entry for the measurement value file. Sufficient data is collected from the user to produce the measurement error and measurement method files. This report is written as a tutorial presenting and explaining all the options available in MFG by giving examples of execution and the resulting screens that MFG produces.

  7. Expression of the nos gene and firefly flashing: a test of the nitric-oxide-mediated flash control model.

    PubMed

    Ohtsuki, Hajime; Yokoyama, Jun; Ohba, Nobuyoshi; Ohmiya, Yoshihiro; Kawata, Masakado

    2014-01-01

    Fireflies (Coleoptera: Lampyridae) emit various types of light that differ among species and populations of the same species. Their lights are assumed to be biological properties that play important ecological and evolutionary roles. Some species in the Lampyridae emit periodic luminescence, the patterns of which are characterized by species-specific intervals. In previous work, it was predicted that the nitric oxide (NO) regulates the oxygen supply required for the bioluminescence reaction of fireflies. Here, the expression of the NO synthase (NOS) mRNA in some fireflies was examined to verify the predictive model of nitric-oxide-mediated flash control in these insects. The expression of the nos gene in the lantern organ was observed not only in nocturnal flashing species but also in diurnal non-flashing species. It was shown that the expression levels of nos were higher in the lantern of Luciola cruciata (Motschulsky) larvae, which that emits continuous light, than in other body parts, although expression in the lantern of the adults, who flash periodically, was not high. Furthermore, there was no significant difference in expression levels among adults of Luciola cruciata characterized by different flashing intervals. The data do not support the model of an NO-mediated flash control mechanism, during which oxygen becomes available for the luciferin-luciferase reaction through NO-mediated inhibition of mitochondrial respiration. It is also indicated that flash patterns do not co-vary with NOS production. However, high nos expression in the larval lantern suggests that NO may play a role in producing continuous light by functioning as a neurotransmitter signal for bioluminescence. PMID:25373203

  8. The dual role of iNOS in cancer☆

    PubMed Central

    Vanini, Frederica; Kashfi, Khosrow; Nath, Niharika

    2015-01-01

    Nitric oxide (NO) is one of the 10 smallest molecules found in nature. It is a simple gaseous free radical whose predominant functions is that of a messenger through cGMP. In mammals, NO is synthesized by the enzyme nitric oxide synthase (NOS) of which there are three isoforms. Neuronal (nNOS, NOS1) and endothelial (eNOS, NOS3) are constitutive calcium-dependent forms of the enzyme that regulate neural and vascular function respectively. The third isoform (iNOS, NOS2), is calcium-independent and is inducible. In many tumors, iNOS expression is high, however, the role of iNOS during tumor development is very complex and quite perplexing, with both promoting and inhibiting actions having been described. This review will aim to summarize the dual actions of iNOS-derived NO showing that the microenvironment of the tumor is a contributing factor to these observations and ultimately to cellular outcomes. PMID:26335399

  9. Ivabradine Prevents Low Shear Stress Induced Endothelial Inflammation and Oxidative Stress via mTOR/eNOS Pathway

    PubMed Central

    Li, Bing; Zhang, Junxia; Wang, Zhimei; Chen, Shaoliang

    2016-01-01

    Ivabradine not only reduces heart rate but has other cardiac and vascular protective effects including anti-inflammation and anti-oxidation. Since endothelial nitric oxide synthase (eNOS) is a crucial enzyme in maintaining endothelial activity, we aimed to investigate the impact of ivabradine in low shear stress (LSS) induced inflammation and endothelial injury and the role of eNOS played in it. Endothelial cells (ECs) were subjected to LSS at 2dyne/cm2, with 1 hour of ivabradine (0.04μM) or LY294002 (10μM) pre-treatment. The mRNA expression of IL-6, VCAM-1 along with eNOS were measured by QPCR. Reactive oxygen species (ROS) was detected by dihydroethidium (DHE) and DCF, and protein phosphorylation was detected by western blot. It demonstrated that ivabradine decreased LSS induced inflammation and oxidative stress in endothelial cells. Western blot showed reduced rictor and Akt-Ser473 as well as increased eNOS-Thr495 phosphorylation. However, mTORC1 pathway was only increased when LSS applied within 30 minutes. These effects were reversed by ivabradine. It would appear that ivabradine diminish ROS generation by provoking mTORC2/Akt phosphorylation and repressing mTORC1 induced eNOS-Thr495 activation. These results together suggest that LSS induced endothelial inflammation and oxidative stress are suppressed by ivabradine via mTORC2/Akt activation and mTORC1/eNOS reduction. PMID:26890696

  10. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... file electronically under 11 CFR 104.18, may file 24-hour reports using the Commission's website's on... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or...., Washington, DC 20510 as required by 11 CFR part 105, by the close of business on the prescribed filing...

  11. Specifying PDD-NOS: A Comparison of PDD-NOS, Asperger Syndrome, and Autism

    ERIC Educational Resources Information Center

    Walker, Darlene R.; Thompson, Ann; Zwaigenbaum, Lonnie; Goldberg, Jeremy; Bryson, Susan E.; Mahoney, William J.; Strawbridge, Christina P.; Szatmari, Peter

    2004-01-01

    Objective: To describe the clinical characteristics of children given a diagnosis of pervasive developmental disorder-not otherwise specified (PDD-NOS) by expert clinicians and to compare these to the clinical characteristics of children given a diagnosis of autism and Asperger syndrome (AS). Method: Two hundred sixteen children with autism, 33…

  12. 75 FR 39206 - Marine Mammals; File Nos. 87-1851 and 555-1870

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-08

    ... No. 87-1851- 02, issued December 28, 2009 (75 FR 106) and Permit No. 555-1870-01, issued February 24, 2010 (75 FR 11132) is requested under the authority of the Marine Mammal Protection Act of 1972, as... hereby given that Daniel P. Costa, PhD, University of California at Santa Cruz, Long Marine...

  13. 77 FR 12010 - Marine Mammals; File Nos. 1076-1789 and 14502

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... (72 FR 13092), authorized the receipt, import and export of marine mammal specimens (cetaceans and... June 17, 2011 (72 FR 13092), authorized the importation of samples from Risso's (Grampus griseus..., Colorado 80234 (Kristi West, Ph.D., Principal Investigator) and Russell Fielding, Ph.D., Department...

  14. 78 FR 30872 - Marine Mammals; File Nos. 14451, 14353, and 13846

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ..., 2013, notice was published in the Federal Register (78 FR 2955) that the subject amendment requests had..., issued on July 14, 2010 (75 FR 43151), authorizes the permit holder, Joseph Mobley, Jr., to study... Insular Hawaiian false killer whales under the ESA. Permit No. 14353, issued on July 14, 2010 (75 FR...

  15. 75 FR 43150 - Marine Mammals; File Nos. 10018, 13846, 14451, 14585, 14599, 14122, 14296, and 14353

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... INFORMATION: On November 12, 2009, notice was published in the Federal Register (74 FR 58243) that requests... amendment to Permit No. 10018, originally issued on June 18, 2008 (73 FR 36042). Permit No. 10018 authorized Dr. Cartwright to conduct humpback whale (Megaptera novaeangliae) research, consisting of...

  16. 77 FR 35657 - Marine Mammals; File Nos. 16163, 16160, and 15569

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... FR 213) that a request for permits to conduct research on marine mammals had been submitted by the... suction- cup tagged. Ultrasound sampling will be directed at killer whales including the Southern Resident stock. Active acoustic playback studies will be directed at Southern Resident killer whales. Import...

  17. 76 FR 62798 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... filing per 154.402: GSS LSS SS-2 S-2 2011 TGPL ACA Tracker Filing to be effective 10/1/2011. Filed Date... Tracker Filing to be effective 11/1/ 2011. Filed Date: 09/30/2011. Accession Number: 20110930-5048.... Description: Texas Gas Transmission, LLC submits tariff filing per 154.403(d)(2): 2011 Fuel Tracker Filing...

  18. Converting CSV Files to RKSML Files

    NASA Technical Reports Server (NTRS)

    Trebi-Ollennu, Ashitey; Liebersbach, Robert

    2009-01-01

    A computer program converts, into a format suitable for processing on Earth, files of downlinked telemetric data pertaining to the operation of the Instrument Deployment Device (IDD), which is a robot arm on either of the Mars Explorer Rovers (MERs). The raw downlinked data files are in comma-separated- value (CSV) format. The present program converts the files into Rover Kinematics State Markup Language (RKSML), which is an Extensible Markup Language (XML) format that facilitates representation of operations of the IDD and enables analysis of the operations by means of the Rover Sequencing Validation Program (RSVP), which is used to build sequences of commanded operations for the MERs. After conversion by means of the present program, the downlinked data can be processed by RSVP, enabling the MER downlink operations team to play back the actual IDD activity represented by the telemetric data against the planned IDD activity. Thus, the present program enhances the diagnosis of anomalies that manifest themselves as differences between actual and planned IDD activities.

  19. Uric acid enhances PKC-dependent eNOS phosphorylation and mediates cellular ER stress: A mechanism for uric acid-induced endothelial dysfunction

    PubMed Central

    LI, PENG; ZHANG, LINA; ZHANG, MEI; ZHOU, CHANGYONG; LIN, NAN

    2016-01-01

    The mechanism by which hyperuricemia induced-endothelial dysfunction contributes to cardiovascular diseases (CVDs) is not yet fully understood. In the present study, we used uric acid (UA) to trigger endothelial dysfunction in cultured endothelial cells, and investigated the effects of induced reactive oxygen species (ROS) generation, endoplasmic reticulum (ER) stress induction, and the protein kinase C (PKC)-dependent endothelial nitric oxide synthase (eNOS) signaling pathway. Human umbilical vein endothelial cells (HUVECs) were incubated with 6, 9 or 12 mg/dl UA, ROS scavenger polyethylene glycol-superoxide dismutase (PEG-SOD), ER stress inhibitor 4-phenylbutyric acid (4-PBA), and PKC inhibitor polymyxin B for 6–48 h. Nitric oxide (NO) production, eNOS activity, intracellular ROS, ER stress levels, and the interaction between eNOS and calmodulin (CaM) and cytosolic calcium levels were assessed using fluorescence microscopy and western blot analysis. Apoptosis was assessed by annexin V staining. UA increased HUVEC apoptosis and reduced eNOS activity and NO production in a dose- and time-dependent manner. Intracellular ROS was elevated after 3 h, while ER stress level increased after 6 h. UA did not alter intracellular Ca2+, CaM, or eNOS concentration, or eNOS Ser1177 phosphorylation. However, PKC-dependent eNOS phosphorylation at Thr495 was greatly enhanced, and consequently interaction between eNOS and CaM was reduced. Cellular ROS depletion, ER stress inhibition and PKC activity reduction inhibited the effect of UA on eNOS activity, NO release and apoptosis in HUVECs. Thus, we concluded that UA induced HUVEC apoptosis and endothelial dysfunction by triggering oxidative and ER stress through PKC/eNOS-mediated eNOS activity and NO production. PMID:26935704

  20. A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense

    PubMed Central

    Jiang, Qiufen; Zhou, Zhi; Wang, Leilei; Wang, Lingling; Yue, Feng; Wang, Jingjing; Song, Linsheng

    2013-01-01

    Background Nitric oxide synthase (NOS) is responsible for synthesizing nitric oxide (NO) from L-arginine, and involved in multiple physiological functions. However, its immunological role in mollusc was seldom reported. Methodology In the present study, an NOS (CfNOS) gene was identified from the scallop Chlamys farreri encoding a polypeptide of 1486 amino acids. Its amino acid sequence shared 50.0~54.7, 40.7~47.0 and 42.5~44.5% similarities with vertebrate neuronal (n), endothelial (e) and inducible (i) NOSs, respectively. CfNOS contained PDZ, oxygenase and reductase domains, which resembled those in nNOS. The CfNOS mRNA transcripts expressed in all embryos and larvae after the 2-cell embryo stage, and were detectable in all tested tissues with the highest level in the gonad, and with the immune tissues hepatopancreas and haemocytes included. Moreover, the immunoreactive area of CfNOS distributed over the haemocyte cytoplasm and cell membrane. After LPS, β-glucan and PGN stimulation, the expression level of CfNOS mRNA in haemocytes increased significantly at 3 h (4.0-, 4.8- and 2.7-fold, respectively, P < 0.01), and reached the peak at 12 h (15.3- and 27.6-fold for LPS and β-glucan respectively, P < 0.01) and 24 h (17.3-fold for PGN, P < 0.01). In addition, TNF-α also induced the expression of CfNOS, which started to increase at 1 h (5.2-fold, P < 0.05) and peaked at 6 h (19.9-fold, P < 0.01). The catalytic activity of the native CfNOS protein was 30.3 ± 0.3 U mgprot-1, and it decreased significantly after the addition of the selective inhibitors of nNOS and iNOS (26.9 ± 0.4 and 29.3 ± 0.1 U mgprot-1, respectively, P < 0.01). Conclusions These results suggested that CfNOS, with identical structure with nNOS and similar enzymatic characteristics to nNOS and iNOS, played the immunological role of iNOS to be involved in the scallop immune defense against PAMPs and TNF-α. PMID:23922688

  1. 77 FR 20090 - Self-Regulatory Organizations; NASDAQ OMX PHLX LLC; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-03

    ... extended through June 30, 2012. See Securities Exchange Act Release Nos. 55153 (January 23, 2007), 72 FR...); 60873 (October 23, 2009), 74 FR 56675 (November 2, 2009) (SR-Phlx-2009-91) (notice of filing and immediate effectiveness expanding and extending Penny Pilot); 60966 (November 9, 2009), 74 FR...

  2. 78 FR 71690 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-29

    ... Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008) (SR-NASDAQ-2008-026) (notice of filing and immediate effectiveness establishing Penny Pilot); 60874 (October 23, 2009), 74 FR... and extending Penny Pilot); 60965 (November 9, 2009), 74 FR 59292 (November 17, 2009)...

  3. 77 FR 40127 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-06

    .... See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008) (SR... 23, 2009), 74 FR 56682 (November 2, 2009) (SR-NASDAQ-2009-091) (notice of filing and immediate effectiveness expanding and extending Penny Pilot); 60965 (November 9, 2009), 74 FR 59292 (November 17,...

  4. 78 FR 37863 - Self-Regulatory Organizations; NASDAQ OMX PHLX LLC; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-24

    ... Securities Exchange Act Release Nos. 55153 (January 23, 2007), 72 FR 4553 (January 31, 2007) (SR-Phlx- 2006... FR 77174 (December 31, 2012) (SR-Phlx-2012-143) (notice of filing and immediate effectiveness... of Penny Pilot Issues That Have Been Delisted June 18, 2013. Pursuant to Section 19(b)(1) of...

  5. 78 FR 77509 - Self-Regulatory Organizations; NASDAQ OMX PHLX LLC; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-23

    ... Act Release Nos. 55153 (January 23, 2007), 72 FR 4553 (January 31, 2007)(SR-Phlx- 2006-74) (notice of filing and approval order establishing Penny Pilot); and 69786 (June 18, 2013), 78 FR 37863 (June 24... Exchange Act Release No. 61061 (November 24, 2009), 74 FR 62857 (December 1, 2009)...

  6. 76 FR 60103 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-28

    ... December 31, 2011. See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4...); 60874 (October 23, 2009), 74 FR 56682 (November 2, 2009) (SR-NASDAQ-2009-091) (notice of filing and immediate effectiveness expanding and extending Penny Pilot); 60965 (November 9, 2009), 74 FR...

  7. 77 FR 63384 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-16

    ... 31, 2012. See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008... (October 23, 2009), 74 FR 56682 (November 2, 2009) (SR-NASDAQ-2009-091) (notice of filing and immediate effectiveness expanding and extending Penny Pilot); 60965 (November 9, 2009), 74 FR 59292 (November 17,...

  8. 77 FR 19396 - Self-Regulatory Organizations; New York Stock Exchange LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ... Reference Room. \\4\\ See Securities Exchange Act Release No. 65562 (October 14, 2011), 76 FR 65288 (October... FR 3297 (January 23, 2012) (File Nos. SR-EDGA-2011-34; SR- EDGX-2011-33; SR-ISE-2011-69; SR-NYSE-2011... Between NYSE Euronext and Deutsche B rse AG Will Not Become Effective March 26, 2012. Pursuant to...

  9. 77 FR 22042 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing of Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-12

    ... market maker. See Securities Exchange Act Release Nos. 60534 (August 19, 2009), 74 FR 44410 (August 28... into the Consolidated FINRA Rulebook as Rule 5250); and 38812 (July 3, 1997), 62 FR 37105 (July 10... Securities Exchange Act Release No. 53128 (January 13, 2006), 71 FR 3550 (January 23, 2006) (File No....

  10. 75 FR 3513 - Self-Regulatory Organizations; Notice of Filing and Immediate Effectiveness of a Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-21

    ... Securities Exchange Act Release No. 58630 (September 24, 2008), 73 FR 57166 (October 1, 2008) (File No. 4-443... Securities Exchange Act Release No. 57478 (March 12, 2008), 73 FR 14521 (March 18, 2008) (SR-NASDAQ- 2007-004.... See Securities Exchange Act Release Nos. 58093 (July 3, 2008), 73 FR 39756 (July 10,...

  11. 77 FR 15153 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing of Proposed Rule Change by...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-14

    ... (October 7, 2011), 76 FR 63977 (October 14, 2011) (SR-BATS-2011-040) (Notice of Filing and Immediate...). \\3\\ See Securities Exchange Act Release No. 62716 (August 13, 2010), 75 FR 51295 (August 19, 2010...). \\4\\ See Securities Exchange Act Release Nos. 62901 (September 13, 2010), 75 FR 57097 (September...

  12. 77 FR 29730 - Self-Regulatory Organizations; NASDAQ OMX BX, Inc.; Notice of Filing of Proposed Rule Change, as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... (January 26, 2012), 77 FR 4841 (January 31, 2012) (BOX Options Exchange LLC; Notice of Filing of... Act Release No. 58135 (July 10, 2008), 73 FR 40898 (July 16, 2008) (SR-NASDAQ-2008-061) (Permitting... NOM and PHLX. See Securities Exchange Act Release Nos. 59995 (May 28, 2009), 74 FR 26750 (June 3,...

  13. 75 FR 34196 - Self-Regulatory Organizations; Notice of Filing of Proposed Rule Change by EDGX Exchange, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... Organization's Statement of the Terms of Substance of the Proposed Rule Change EDGX proposes to make changes to...-Regulatory Organization's Statement of the Purpose of, and Statutory Basis for, the Proposed Rule Change 1... Exchange Release No. 61698 (March 12, 2010), 75 FR 13151 (March 18, 2010) (approving File Nos. 10-194...

  14. 75 FR 34189 - Self-Regulatory Organizations; Notice of Filing of Proposed Rule Change by EDGA Exchange, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... Organization's Statement of the Terms of Substance of the Proposed Rule Change EDGA proposes to make changes to...-Regulatory Organization's Statement of the Purpose of, and Statutory Basis for, the Proposed Rule Change 1... Exchange Release No. 61698 (March 12, 2010), 75 FR 13151 (March 18, 2010) (approving File Nos. 10-194...

  15. 76 FR 51114 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-17

    ...). \\12\\ See Securities Exchange Act Release Nos. 61483 (February 3, 2010), 75 FR 6753 (February 10, 2010..., 2010), 75 FR 40007 (July 13, 2010) (SR-BX-2010-045) (rule filings to enable the listing and trading of... Effectiveness of Proposed Rule Change Relating to Options on ETFS Gold Trust August 11, 2011. Pursuant...

  16. 77 FR 45706 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing of Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-01

    ... Release No. 53128 (January 13, 2006), 71 FR 3550 (January 23, 2006) (File No. 10-131). The rule was amended in Securities Exchange Act Release Nos. 54155 (July 14, 2006), 71 FR 41291 (July 20, 2006) (SR-NASDAQ-2006-001); 60794 (October 6, 2009), 74 FR 52522 (October 13, 2009) (SR-NASDAQ-2009-084); and...

  17. 77 FR 42541 - Self-Regulatory Organizations; NASDAQ OMX PHLX LLC; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-19

    ... Securities Exchange Act Release Nos. 55153 (January 23, 2007), 72 FR 4553 (January 31, 2007)(SR-Phlx-2006-74)(notice of filing and approval order establishing Penny Pilot); 60873 (October 23, 2009), 74 FR 56675... Penny Pilot); 60966 (November 9, 2009), 74 FR 59331 (November 17, 2009)(SR-Phlx-2009- 94)(notice...

  18. 77 FR 36310 - Self-Regulatory Organizations; NASDAQ OMX PHLX LLC; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ... Exchange Act Release Nos. 55153 (January 23, 2007), 72 FR 4553 (January 31, 2007) (SR-Phlx-2006-74) (notice of filing and approval order establishing Penny Pilot); 60873 (October 23, 2009), 74 FR 56675... Penny Pilot); 60966 (November 9, 2009), 74 FR 59331 (November 17, 2009) (SR-Phlx-2009-94) (notice...

  19. 78 FR 7822 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-04

    ... Market Volatility. \\3\\ See Securities Exchange Act Release No. 67565 (August 1, 2012), 77 FR 47144... (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014; SR-EDGA-2010-01; SR-EDGX- 2010... Release No. 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010-025). See also...

  20. 78 FR 50465 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... effectiveness adding seventy-five classes to Penny Pilot); 62029 (May 4, 2010), 75 FR 25895 (May 10, 2010) (SR... Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008) (SR-NASDAQ-2008-026) (notice of filing and immediate effectiveness establishing Penny Pilot); 60874 (October 23, 2009), 74...

  1. 78 FR 75949 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-13

    ... seventy-five classes to Penny Pilot); 65969 (December 15, 2011), 76 FR 79268 (December 21, 2011) (SR... 31, 2013. See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008... (October 23, 2009), 74 FR 56682 (November 2, 2009) (SR-NASDAQ-2009-091) (notice of filing and...

  2. 76 FR 27680 - Self-Regulatory Organizations; BATS Y-Exchange, Inc.; Notice of Filing of a Proposed Rule Change...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-12

    ... all equities exchanges and FINRA. See Securities Exchange Act Release No. 62252 (June 10, 2010), 75 FR..., 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010- 025). \\4\\ The Commission approved the addition to...), 75 FR 56618 (September 16, 2010) (File Nos. SR-BATS-2010-018; SR-BX-2010-044; SR-CBOE-2010-065;...

  3. 78 FR 11711 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-19

    ... COMMISSION Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate... thereunder,\\2\\ notice is hereby given that on February 1, 2013, The NASDAQ Stock Market LLC (``NASDAQ'' or... 31, 2012. See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4,...

  4. 78 FR 61418 - Self-Regulatory Organizations; NASDAQ OMX BX, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-03

    ... Release Nos. 67256 (June 26, 2012), 77 FR 39277 (July 2, 2012) (SR-BX-2012-030) (order approving BX Options rules and establishing Penny Pilot); 67342 (July 3, 2012), 77 FR 40666 (July 10, 2012) (SR-BX-2012... (December 21, 2012), 77 FR 77152 (December 31, 2012) (SR-BX-2012- 076) (notice of filing and...

  5. 76 FR 19162 - Self-Regulatory Organizations; NASDAQ OMX BX, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ...\\ Additionally, BOX proposes to specify that it may select up to sixty (60) option classes on individual stocks... Release Nos. 49068 (January 13, 2004) 69 FR 2775 (January 20, 2004) (Order Approving Establishment of BOX Rules) (BSE-2002-15) and 56655 (October 12, 2007) 72 FR 59126 (October 18, 2007) (Notice of Filing...

  6. 77 FR 77141 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing of a Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ..., 2012), 77 FR 22042 (April 12, 2012)(SR-NASDAQ-2012- 043)(notice of filing); and 68378 (December 6, 2012), 77 FR 74042 (December 12, 2012)(notice of withdrawal). Attached hereto is Exhibit 4 that reflects the... Exchange Act Release Nos. 60534 (August 19, 2009), 74 FR 44410 (August 28,...

  7. 78 FR 3060 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC Notice; of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... COMMISSION Self-Regulatory Organizations; The NASDAQ Stock Market LLC Notice; of Filing and Immediate.... 78s(b)(1). \\2\\ 17 CFR 240.19b-4. I. Self-Regulatory Organization's Statement of the Terms of Substance... Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008)...

  8. 77 FR 22015 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-12

    ... COMMISSION Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...,\\2\\ notice is hereby given that on April 2, 2012, The NASDAQ Stock Market LLC (``NASDAQ'' or... extended through June 30, 2012. See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73...

  9. 78 FR 28912 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    .... See Securities Exchange Act Release Nos. 57579 (March 28, 2008), 73 FR 18587 (April 4, 2008) (SR... 23, 2009), 74 FR 56682 (November 2, 2009)(SR-NASDAQ-2009-091) (notice of filing and immediate effectiveness expanding and extending Penny Pilot); 60965 (November 9, 2009), 74 FR 59292 (November 17,...

  10. 78 FR 64569 - Self-Regulatory Organizations; NASDAQ OMX PHLX LLC; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-29

    ... NASDAQ OMX Group.\\3\\ \\3\\ See Securities Exchange Act Release Nos. 70269 (August 27, 2013), 78 FR 54336...), 78 FR 18378 (March 26, 2013) (SR-Phlx- 2013-28) (notice of filing and immediate effectiveness of... Securities Exchange Act Release No. 69041 (March 5, 2013), 78 FR 15791 (March 12, 2013)...

  11. 76 FR 27683 - Self-Regulatory Organizations; Chicago Stock Exchange, Inc.; Notice of Filing of Proposed Change...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-12

    ... Exchange Act Release No. 63498 (December 9, 2010), 75 FR 78310 (December 15, 2010) (SR-CHX-2010-24). Then.... 64203 (April 6, 2011), 76 FR 20393 (April 12, 2011) (SR-CHX-2011-05). The Exchange has continued to... Release No. 62252 (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014;...

  12. 77 FR 15163 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-14

    ... implemented, see Securities Exchange Act Release No. 63270 (November 8, 2010), 75 FR 69489 (November 12, 2010... Securities Exchange Act Release Nos. 63414 (December 2, 2010), 75 FR 76505 (December 8, 2010) (NASDAQ-2010-153) (notice of filing and immediate effectiveness); 63628 (January 3, 2011), 76 FR 1201 (January...

  13. 75 FR 76759 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-09

    ... permanent or January 31, 2011. See Securities Exchange Act Release No. 62857 (September 7, 2010), 75 FR... FR 41264 (July 15, 2010) (SR-NYSEAmex-2010-31) (Notice of Filing of Amendment Nos. 2 and 3, and Order... Securities Exchange Act Release No. 58863 (October 27, 2008), 73 FR 65417 (November 3, 2008) (Notice...

  14. 76 FR 28836 - Self-Regulatory Organizations; Notice of Filing of Proposed Rule Change by NASDAQ OMX PHLX LLC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-18

    ... Securities Exchange Act Release No. 57478 (March 12, 2008), 73 FR 14521 (March 18, 2008) (SR-NASDAQ-2007-004... (January 11, 2011), 76 FR 2931 (January 18, 2011) (SR-Phlx-2011-04) (notice of filing and immediate... Exchange Act Release Nos. 62296 (June 15, 2010), 75 FR 35115 (June 21, 2010) (SR-Phlx-2010-84) (notice...

  15. 78 FR 14141 - Self-Regulatory Organizations; NASDAQ OMX BX, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-04

    ..., membership fees at away markets, Options Regulatory Fees (``ORFs'') and technical costs associated with... and technical costs associated with operating NOS, membership fees at away markets, ORFs ] and... No. 68025 (October 10, 2012), 77 FR 63398 (October 16, 2012) (SR-OCC-2012-18). C2 recently filed...

  16. 77 FR 16283 - Self-Regulatory Organizations; NASDAQ OMX PHLX LLC; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ... Act Release Nos. 55153 (January 23, 2007), 72 FR 4553 (January 31, 2007) (SR-Phlx-2006-74) (notice of filing and approval order establishing Penny Pilot); 60873 (October 23, 2009), 74 FR 56675 (November 2... Pilot); 60966 (November 9, 2009), 74 FR 59331 (November 17, 2009) (SR-Phlx- 2009-94) (notice of...

  17. 75 FR 27603 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-17

    ... September, 2008. See Securities Exchange Act Release No. 58516 (September 11, 2008), 73 FR 54184 (September... Securities Exchange Act Release Nos. 58571 (September 17, 2008), 73 FR 55188 (September 24, 2008) (SR-Phlx-2008-60) (notice of filing and immediate effectiveness); 59923 (May 14, 2009), 74 FR 23902 (May...

  18. 75 FR 27608 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-17

    ... established in July 2008. See Securities Exchange Act Release No. 58203 (July 22, 2008), 73 FR 43812 (July 28... Exchange Act Release Nos. 58571 (September 17, 2008), 73 FR 55188 (September 24, 2008) (SR-Phlx- 2008-60) (notice of filing and immediate effectiveness); 59923 (May 14, 2009), 74 FR 23902 (May 21, 2009)...

  19. Viscum album aqueous extract induces NOS-2 and NOS-3 overexpression in Guinea pig hearts.

    PubMed

    Tenorio-Lopez, Fermin Alejandro; Valle Mondragon, Leonardo Del; Olvera, Gabriela Zarco; Torres Narvaez, Juan Carlos; Pastelin, Gustavo

    2006-11-01

    Viscum album L. aqueous extract, on the Langendorff isolated and perfused heart model, decreases coronary vascular resistance, when compared to control group (36.00 +/- 2.00 vs. 15.80 +/- 1.96 dyn s cm-5). Our data support the fact that this mechanism involves NOS-2 and NOS-3 overexpression (4.65 and 7.89 times over control, respectively), which is correlated with increases in NO (6.24 +/- 2.49 vs. 147.95 +/- 2.79 pmol) and cGMP production (43.94 +/- 2.00 vs. 74.81 +/- 1.96 pmol mg-1 of tissue), compared to control values. Such an effect is antagonized by gadolinium(III) chloride, L-NAME and ODQ. Therefore, coronary vasodilator effect elicited by V. album L. aqueous extract is mediated by the NO/sGC pathway. PMID:17127505

  20. Advancing the perceptions of the nature of science (NOS): integrating teaching the NOS in a science content course

    NASA Astrophysics Data System (ADS)

    Aflalo, Ester

    2014-09-01

    Background:Understanding the nature of science (NOS) has been a key objective in teaching sciences for many years. Despite the importance of this goal it is, until this day, a complex challenge that we are far from achieving. Purpose:The study was conducted in order to further the understanding of the NOS amongst preservice teachers. It explores the effects of another approach to teaching that combines teaching the NOS in a course of scientific content. Sample and Programmed description:109 preservice teachers studied the course on 'Cell Biology' or the course, 'Introduction to Life Sciences', whose teaching model differs. In addition to the usual subject matter the courses included activities to understand some aspects of the NOS, reflective discussions, as well as historical descriptions of the scientific discoveries and developments pertaining to the course matter. Design and methods:The study has characteristics of action research The perceptions of the NOS amongst preservice teachers were examined prior to, and following, the course. The perceptions were examined through 35 closed questions and one open question. Results:The findings show that following the course, some of the concepts of NOS changed. Naïve and conservative acuity developed into more current perceptions towards the NOS. Similarly, there was greater internalization of the meaning of the NOS and the significance of its teaching. Conclusions:The findings of this research discuss evidence of the importance of combining the teaching of the NOS in scientific courses in order to advance scientific literacy.

  1. File Management In Space

    NASA Technical Reports Server (NTRS)

    Critchfield, Anna R.; Zepp, Robert H.

    2000-01-01

    We propose that the user interact with the spacecraft as if the spacecraft were a file server, so that the user can select and receive data as files in standard formats (e.g., tables or images, such as jpeg) via the Internet. Internet technology will be used end-to-end from the spacecraft to authorized users, such as the flight operation team, and project scientists. The proposed solution includes a ground system and spacecraft architecture, mission operations scenarios, and an implementation roadmap showing migration from current practice to the future, where distributed users request and receive files of spacecraft data from archives or spacecraft with equal ease. This solution will provide ground support personnel and scientists easy, direct, secure access to their authorized data without cumbersome processing, and can be extended to support autonomous communications with the spacecraft.

  2. Upregulated iNOS and oxidative damage to the cochlear stria vascularis due to noise stress.

    PubMed

    Shi, Xiaorui; Nuttall, Alfred L

    2003-03-28

    Our previous work has revealed increased nitric oxide (NO) production in the cochlear perilymph following noise stress. However, it is not clear if the increase of NO is related to iNOS and whether NO-related oxidative stress can cause vascular tissue damage. In this study, iNOS immunoreactivity, NO production, and reactive oxygen species (ROS) in the lateral wall were examined in normal mice and compared with similar animals exposed to 120 dBA broadband noise, 3 h/day, for 2 consecutive days. In the normal animals, iNOS expression was not observed in the vascular endothelium of the stria vascularis and only weak iNOS immunoactivity was detected in the marginal cells. However, expression of iNOS in the wall of the blood vessels of stria vascularis and marginal cells was observed after loud sound stress (LSS). Relatively low levels of NO production and low ROS activity were detected in the stria vascularis in the unstimulated condition. In contrast, NO production was increased and ROS activity was elevated in the stria vascularis after LSS. These changes were attenuated by the iNOS inhibitor, GW 274150. To explore whether noise induces apoptotic processes in the stria vascularis, we examined morphological changes in endothelial- and marginal-cells. In vitro, annexin-V phosphatidylserine (PS) (to label and detect early evidence of apoptosis) was combined with propidium iodide (PI) (to probe plasma membrane integrity). PI alone was used in fixed tissues to detect later stage apoptotic cells by morphology of the nuclei. Following LSS, PS was expressed on cell surfaces of endothelial cells of blood vessels and marginal cells of the stria vascularis. Later stage apoptosis, characterized by irregular nuclei and condensation of nuclei, was also observed in these cells. The data indicate that increased iNOS expression and production of both NO and ROS following noise stress may lead to marginal cell pathology, and the dysfunction of cochlear microcirculation by inducing

  3. 76 FR 68436 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-04

    ... tariff filing per 154.204: 2011 SoCal Nonconforming Agreement Vintage to be effective 11/1/2011. Filed...://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676...

  4. NASA Thesaurus Data File

    NASA Technical Reports Server (NTRS)

    2012-01-01

    The NASA Thesaurus contains the authorized NASA subject terms used to index and retrieve materials in the NASA Aeronautics and Space Database (NA&SD) and NASA Technical Reports Server (NTRS). The scope of this controlled vocabulary includes not only aerospace engineering, but all supporting areas of engineering and physics, the natural space sciences (astronomy, astrophysics, planetary science), Earth sciences, and the biological sciences. The NASA Thesaurus Data File contains all valid terms and hierarchical relationships, USE references, and related terms in machine-readable form. The Data File is available in the following formats: RDF/SKOS, RDF/OWL, ZThes-1.0, and CSV/TXT.

  5. XML Files: MedlinePlus

    MedlinePlus

    ... page: https://medlineplus.gov/xml.html MedlinePlus XML Files To use the sharing features on this page, ... information on all English and Spanish topic groups. Files generated on September 15, 2016 MedlinePlus Health Topic ...

  6. Face and Emotion Recognition in MCDD versus PDD-NOS

    ERIC Educational Resources Information Center

    Herba, Catherine M.; de Bruin, Esther; Althaus, Monika; Verheij, Fop; Ferdinand, Robert F.

    2008-01-01

    Previous studies indicate that Multiple Complex Developmental Disorder (MCDD) children differ from PDD-NOS and autistic children on a symptom level and on psychophysiological functioning. Children with MCDD (n = 21) and PDD-NOS (n = 62) were compared on two facets of social-cognitive functioning: identification of neutral faces and facial…

  7. Multiple Complex Developmental Disorder Delineated from PDD-NOS

    ERIC Educational Resources Information Center

    de Bruin, Esther I.; de Nijs, Pieter F. A.; Verheij, Fop; Hartman, Catharina A.; Ferdinand, Robert F.

    2007-01-01

    The objective of this study was to identify behavioral differences between children with multiple complex developmental disorder (MCDD) and those with pervasive developmental disorder-not otherwise specified (PDD-NOS). Twenty-five children (6-12 years) with MCDD and 86 children with PDD-NOS were compared with respect to psychiatric co-morbidity,…

  8. 15 CFR Supplement Nos. 2-3 to Part... - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Nos. Supplement Nos. 2-3 to Part 716 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS INITIAL AND...

  9. Magnetic circular dichroism spectroscopic characterization of the NOS-like protein from Geobacillus stearothermophilus (gsNOS).

    PubMed

    Kinloch, Ryan D; Sono, Masanori; Sudhamsu, Jawahar; Crane, Brian R; Dawson, John H

    2010-03-01

    Nitric oxide synthase (NOS) catalyzes the NADPH- and O(2)-dependent oxidation of l-arginine (l-Arg) to nitric oxide (NO) and citrulline via an N(G)-hydroxy-l-arginine (NHA) intermediate. Mammalian NOSs have been studied quite extensively; other eukaryotes and some prokaryotes appear to express NOS-like proteins comparable to the oxygenase domain of mammalian NOSs. In this study, a recombinant NOS-like protein from the thermostable bacterium Geobacillus stearothermophilus (gsNOS) has been characterized using magnetic circular dichroism (MCD) and UV-Vis absorption spectroscopic techniques. Spectral comparisons of ligand complexes (with O(2), NO and CO) of substrate-bound (l-Arg or NHA) gsNOS, including the key oxyferrous complex studied at -50 degrees C in cryogenic mixed solvents, with analogous mammalian NOS complexes indicate overall spectroscopic similarities between gsNOS and mammalian NOSs. However, more detailed spectral comparisons reflect subtle structural differences between gsNOS and mammalian NOSs. This may be due to an incomplete tetrahydrobiopterin (BH(4))-binding site and low BH(4)-binding affinity, which may become even lower in the presence of cryosolvent in gsNOS. Although BH(4)-binding may be altered, gsNOS appears to require the pterin for NO production since formation of the stable ferric-NO product complex was only observed when excess BH(4) (>150muM) over gsNOS was present upon single turnover reaction in which O(2) was bubbled into dithionite-reduced NHA-bound protein solution at -35 degrees C or -50 degrees C. PMID:20110129

  10. 76 FR 348 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-04

    ... Transmission, LLC submits tariff filing per 154.204: AGT Modifications for Bobcat's Link Implementation Filing... Transmission, L.L.C. submits tariff filing per 154.204: Modifications for Bobcat's LINK Implementation Filing... Transmission, LP submits tariff filing per 154.204: TETLP Filing for Bobcat's Link Implementation to...

  11. 76 FR 50210 - Combined Notice of Filings #

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-12

    ... 35.13(a)(2)(iii: Filing of Notice of Succession to be effective 10/5/2011. Filed Date: 08/05/2011....13(a)(2)(iii: Filing of Notice of Succession to be effective 10/5/2011. Filed Date: 08/05/2011....13(a)(2)(iii: Filing of Notice of Succession to be effective 10/5/2011. Filed Date:...

  12. Endothelial function does not improve with high-intensity continuous exercise training in SHR: implications of eNOS uncoupling.

    PubMed

    Battault, Sylvain; Singh, François; Gayrard, Sandrine; Zoll, Joffrey; Reboul, Cyril; Meyer, Grégory

    2016-02-01

    Exercise training is a well-recognized way to improve vascular endothelial function by increasing nitric oxide (NO) bioavailability. However, in hypertensive subjects, unlike low- and moderate-intensity exercise training, the beneficial effects of continuous high-intensity exercise on endothelial function are not clear, and the underlying mechanisms remain unknown. The aim of this study was to investigate the impact of high-intensity exercise on vascular function, especially on the NO pathway, in spontaneous hypertensive rats (SHR). These effects were studied on WKY, sedentary SHR and SHR that exercised at moderate (SHR-MOD) and high intensity (SHR-HI) on a treadmill (1 h per day; 5 days per week for 6 weeks at 55% and 80% of their maximal aerobic velocity, respectively). Endothelial function and specific NO contributions to acetylcholine-mediated relaxation were evaluated by measuring the aortic ring isometric forces. Endothelial nitric oxide synthase (eNOS) expression and phosphorylation (ser1177) were evaluated by western blotting. The total aortic and eNOS-dependent reactive oxygen species (ROS) production was assessed using electron paramagnetic resonance in aortic tissue. Although the aortas of SHR-HI had increased eNOS levels without alteration of eNOS phosphorylation, high-intensity exercise had no beneficial effect on endothelium-dependent vasorelaxation, unlike moderate exercise. This result was associated with increased eNOS-dependent ROS production in the aortas of SHR-HI. Notably, the use of the recoupling agent BH4 or a thiol-reducing agent blunted eNOS-dependent ROS production in the aortas of SHR-HI. In conclusion, the lack of a positive effect of high-intensity exercise on endothelial function in SHR was mainly explained by redox-dependent eNOS uncoupling, resulting in a switch from NO to O2(-) generation. PMID:26537830

  13. Illegal File Sharing 101

    ERIC Educational Resources Information Center

    Wada, Kent

    2008-01-01

    Much of higher education's unease arises from the cost of dealing with illegal file sharing. Illinois State University, for example, calculated a cost of $76 to process a first claim of copyright infringement and $146 for a second. Responses range from simply passing along claims to elaborate programs architected with specific goals in mind.…

  14. MPI File Tree Walk

    Energy Science and Technology Software Center (ESTSC)

    2007-04-30

    MPI-FTW is a scalable MPI based software application that navigates a directory tree by dynamically allocating processes to navigate sub-directories found. Upon completion, MPI-FTW provides statistics on the number of directories found, files found, and time to complete. Inaddition, commands can be executed at each directory level.

  15. The New Resource File

    ERIC Educational Resources Information Center

    Luck, Donald D.

    2011-01-01

    The development of the resource file is a common experience in teacher preparation programs. The author examines strategies for transforming what has been a project composed of physical resources to one emphasizing digital resources. Methods for finding, tagging, storing and retrieving resources are explored.

  16. Career Education Product File.

    ERIC Educational Resources Information Center

    Michigan State Dept. of Education, Lansing. Office of Career Education.

    Designed for school staffs and others looking for effective career education programs, this resource file contains descriptions of 150 career education programs and products. It provides an overview of the types of research and development-based career education programs currently available to school districts across the United States. The file…

  17. The Global File System

    NASA Technical Reports Server (NTRS)

    Soltis, Steven R.; Ruwart, Thomas M.; OKeefe, Matthew T.

    1996-01-01

    The global file system (GFS) is a prototype design for a distributed file system in which cluster nodes physically share storage devices connected via a network-like fiber channel. Networks and network-attached storage devices have advanced to a level of performance and extensibility so that the previous disadvantages of shared disk architectures are no longer valid. This shared storage architecture attempts to exploit the sophistication of storage device technologies whereas a server architecture diminishes a device's role to that of a simple component. GFS distributes the file system responsibilities across processing nodes, storage across the devices, and file system resources across the entire storage pool. GFS caches data on the storage devices instead of the main memories of the machines. Consistency is established by using a locking mechanism maintained by the storage devices to facilitate atomic read-modify-write operations. The locking mechanism is being prototyped in the Silicon Graphics IRIX operating system and is accessed using standard Unix commands and modules.

  18. "The Bean Files."

    ERIC Educational Resources Information Center

    Haq, Krystyna; Longnecker, Nancy; Hickey, Ruth

    1999-01-01

    Describes classroom use and effectiveness of "The Bean Files," an internet package that uses humorous stories to introduce students to life on a wheat-sheep farm in the Mediterranean climate areas of Australia. The focus of the program is on the role of legume-cereal rotations in the farming system and the science underpinning this agricultural…

  19. Vascular nitric oxide: Beyond eNOS.

    PubMed

    Zhao, Yingzi; Vanhoutte, Paul M; Leung, Susan W S

    2015-10-01

    As the first discovered gaseous signaling molecule, nitric oxide (NO) affects a number of cellular processes, including those involving vascular cells. This brief review summarizes the contribution of NO to the regulation of vascular tone and its sources in the blood vessel wall. NO regulates the degree of contraction of vascular smooth muscle cells mainly by stimulating soluble guanylyl cyclase (sGC) to produce cyclic guanosine monophosphate (cGMP), although cGMP-independent signaling [S-nitrosylation of target proteins, activation of sarco/endoplasmic reticulum calcium ATPase (SERCA) or production of cyclic inosine monophosphate (cIMP)] also can be involved. In the blood vessel wall, NO is produced mainly from l-arginine by the enzyme endothelial nitric oxide synthase (eNOS) but it can also be released non-enzymatically from S-nitrosothiols or from nitrate/nitrite. Dysfunction in the production and/or the bioavailability of NO characterizes endothelial dysfunction, which is associated with cardiovascular diseases such as hypertension and atherosclerosis. PMID:26499181

  20. Accessing files in an internet - The Jade file system

    NASA Technical Reports Server (NTRS)

    Rao, Herman C.; Peterson, Larry L.

    1993-01-01

    Jade is a new distribution file system that provides a uniform way to name and access files in an internet environment. It makes two important contributions. First, Jade is a logical system that integrates a heterogeneous collection of existing file systems, where heterogeneous means that the underlying file systems support different file access protocols. Jade is designed under the restriction that the underlying file system may not be modified. Second, rather than providing a global name space, Jade permits each user to define a private name space. These private name spaces support two novel features: they allow multiple file systems to be mounted under one directory, and they allow one logical name space to mount other logical name spaces. A prototype of the Jade File System was implemented on Sun Workstations running Unix. It consists of interfaces to the Unix file system, the Sun Network File System, the Andrew File System, and FTP. This paper motivates Jade's design, highlights several aspects of its implementation, and illustrates applications that can take advantage of its features.

  1. Accessing files in an Internet: The Jade file system

    NASA Technical Reports Server (NTRS)

    Peterson, Larry L.; Rao, Herman C.

    1991-01-01

    Jade is a new distribution file system that provides a uniform way to name and access files in an internet environment. It makes two important contributions. First, Jade is a logical system that integrates a heterogeneous collection of existing file systems, where heterogeneous means that the underlying file systems support different file access protocols. Jade is designed under the restriction that the underlying file system may not be modified. Second, rather than providing a global name space, Jade permits each user to define a private name space. These private name spaces support two novel features: they allow multiple file systems to be mounted under one directory, and they allow one logical name space to mount other logical name spaces. A prototype of the Jade File System was implemented on Sun Workstations running Unix. It consists of interfaces to the Unix file system, the Sun Network File System, the Andrew File System, and FTP. This paper motivates Jade's design, highlights several aspects of its implementation, and illustrates applications that can take advantage of its features.

  2. Protein Inhibitor of NOS1 Plays a Central Role in the Regulation of NOS1 Activity in Human Dilated Hearts

    PubMed Central

    Roselló-Lletí, Esther; Tarazón, Estefanía; Ortega, Ana; Gil-Cayuela, Carolina; Carnicer, Ricardo; Lago, Francisca; González-Juanatey, Jose Ramón; Portolés, Manuel; Rivera, Miguel

    2016-01-01

    An essential factor for the production of nitric oxide by nitric oxide synthase 1 (NOS1), major modulator of cardiac function, is the cofactor tetrahydrobiopterin (BH4). BH4 is regulated by GTP cyclohydrolase 1, the rate-limiting enzyme in BH4 biosynthesis which catalyses the formation of dihydroneopterin 3′triphosfate from GTP, producing BH4 after two further steps catalyzed by 6-pyruvoyltetrahydropterin synthase and sepiapterin reductase. However, there are other essential factors involved in the regulation of NOS1 activity, such as protein inhibitor of NOS1 (PIN), calmodulin, heat shock protein 90, and NOS interacting protein. All these molecules have never been analysed in human non-ischemic dilated hearts (DCM). In this study we demonstrated that the upregulation of cardiac NOS1 is not accompanied by increased NOS1 activity in DCM, partly due to the elevated PIN levels and not because of alterations in biopterin biosynthesis. Notably, the PIN concentration was significantly associated with impaired ventricular function, highlighting the importance of this NOS1 activity inhibitor in Ca2+ homeostasis. These results take a central role in the current list of targets for future studies focused on the complex cardiac dysfunction processes through more efficient harnessing of NOS1 signalling. PMID:27481317

  3. Advancing the Perceptions of the Nature of Science (NOS): Integrating Teaching the NOS in a Science Content Course

    ERIC Educational Resources Information Center

    Aflalo, Ester

    2014-01-01

    Background: Understanding the nature of science (NOS) has been a key objective in teaching sciences for many years. Despite the importance of this goal it is, until this day, a complex challenge that we are far from achieving. Purpose: The study was conducted in order to further the understanding of the NOS amongst preservice teachers. It explores…

  4. Endothelial NOS-dependent activation of c-Jun NH(2)- terminal kinase by oxidized low-density lipoprotein

    NASA Technical Reports Server (NTRS)

    Go, Y. M.; Levonen, A. L.; Moellering, D.; Ramachandran, A.; Patel, R. P.; Jo, H.; Darley-Usmar, V. M.

    2001-01-01

    Oxidized low-density lipoprotein (oxLDL) is known to activate a number of signal transduction pathways in endothelial cells. Among these are the c-Jun NH(2)-terminal kinase (JNK), also known as stress-activated protein kinase, and extracellular signal-regulated kinase (ERK). These mitogen-activated protein kinases (MAP kinase) determine cell survival in response to environmental stress. Interestingly, JNK signaling involves redox-sensitive mechanisms and is activated by reactive oxygen and nitrogen species derived from both NADPH oxidases, nitric oxide synthases (NOS), peroxides, and oxidized low-density lipoprotein (oxLDL). The role of endothelial NOS (eNOS) in the activation of JNK in response to oxLDL has not been examined. Herein, we show that on exposure of endothelial cells to oxLDL, both ERK and JNK are activated through independent signal transduction pathways. A key role of eNOS activation through a phosphatidylinositol-3-kinase-dependent mechanism leading to phosphorylation of eNOS is demonstrated for oxLDL-dependent activation of JNK. Moreover, we show that activation of ERK by oxLDL is critical in protection against the cytotoxicity of oxLDL.

  5. 43 CFR 4.1371 - Who may file, where to file, when to file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of proposed suspension or rescission under 30 CFR 773.22 or a notice of suspension or rescission under 30 CFR 773.23 may file a request for review with the Hearings Division, Office of Hearings and... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file, where to file, when to...

  6. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or... required by 11 CFR part 105, by the close of business on the prescribed filing date. (b) Timely filed. (1... and in 11 CFR 104.5, (i) Overnight delivery service means a private delivery service business...

  7. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or... required by 11 CFR part 105, by the close of business on the prescribed filing date. (b) Timely filed. (1... and in 11 CFR 104.5, (i) Overnight delivery service means a private delivery service business...

  8. Using a Professional Development Program for Enhancing Chilean Biology Teachers' Understanding of Nature of Science (NOS) and Their Perceptions About Using History of Science to Teach NOS

    NASA Astrophysics Data System (ADS)

    Pavez, José M.; Vergara, Claudia A.; Santibañez, David; Cofré, Hernán

    2016-05-01

    A number of authors have recognized the importance of understanding the nature of science (NOS) for scientific literacy. Different instructional strategies such as decontextualized, hands-on inquiry, and history of science (HOS) activities have been proposed for teaching NOS. This article seeks to understand the contribution of HOS in enhancing biology teachers' understanding of NOS, and their perceptions about using HOS to teach NOS. These teachers ( N = 8), enrolled in a professional development program in Chile are, according to the national curriculum, expected to teach NOS, but have no specific NOS and HOS training. Teachers' views of NOS were assessed using the VNOS-D+ questionnaire at the beginning and at the end of two modules about science instruction and NOS. Both the pre- and the post-test were accompanied by interviews, and in the second session we collected information about teachers' perceptions of which interventions had been more significant in changing their views on NOS. Finally, the teachers also had to prepare a lesson plan for teaching NOS that included HOS. Some of the most important study results were: significant improvements were observed in teachers' understanding of NOS, although they assigned different levels of importance to HOS in these improvements; and although the teachers improved their understanding of NOS, most had difficulties in planning lessons about NOS and articulating historical episodes that incorporated NOS. The relationship between teachers' improved understanding of NOS and their instructional NOS skills is also discussed.

  9. Ursolic acid from the Chinese herb danshen (Salvia miltiorrhiza L.) upregulates eNOS and downregulates Nox4 expression in human endothelial cells.

    PubMed

    Steinkamp-Fenske, Katja; Bollinger, Larissa; Völler, Natalie; Xu, Hui; Yao, Ying; Bauer, Rudolf; Förstermann, Ulrich; Li, Huige

    2007-11-01

    Danshen, the dried root of Salvia miltiorrhiza Bunge (Lamiaceae), is one of the most commonly used traditional Chinese medicines for cardiovascular indications. In EA.hy 926 cells, a cell line derived from human umbilical vein endothelial cells (HUVEC), an aqueous extract of danshen, and also a methanol extract of the plant, increased eNOS promoter activity, eNOS mRNA and protein expression, as well as endothelial NO production. A dichloromethane extract, in contrast, did not change eNOS gene expression. Thus, the active danshen constituent(s) responsible for eNOS upregulation is (are) hydrophilic and/or alcohol-soluble. One such compound is ursolic acid that significantly increased eNOS expression in EA.hy 926 cells and native HUVEC, and enhanced bioactive NO production measured in terms of its cGMP increasing activity. Other tested hydrophilic and alcohol-soluble compounds isolated from danshen had no effect on eNOS expression. Interestingly, ursolic acid also reduced the expression of the NADPH oxidase subunit Nox4 and suppressed the production of reactive oxygen species in human endothelial cells. Upregulation of eNOS and a parallel downregulation of Nox4 lead to an increase in bioactive NO. This in turn could mediate some of the beneficial effects of danshen. Ursolic acid is a prototypical compound responsible for this effect of the plant. PMID:17481637

  10. 78 FR 52190 - United States Department of Energy, Bonneville Power Administration: Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket Nos. EF13-7-000, EF13-8-000, EF13-9-000, EF13-10-000, EF13-11- 000, EF13-12-000, EF13-13-000, EF13-14-000] United States Department of Energy, Bonneville Power Administration: Notice of Filing Take notice that...

  11. 78 FR 10653 - Self-Regulatory Organizations; EDGA Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-14

    ... Release No. 67501 (July 25, 2012), 77 FR 45396 (July 31, 2012) (SR-EDGA-2012-31). EDGA Rule 11.14 requires..., 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014; SR-EDGA-2010-01; SR-EDGX- 2010-01; SR.... 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010-025). See also Securities Exchange...

  12. 78 FR 10682 - Self-Regulatory Organizations; EDGX Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-14

    ... Release No. 67502 (July 25, 2012), 77 FR 45394 (July 31, 2012) (SR-EDGX-2012-28). EDGX Rule 11.14 requires..., 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014; SR-EDGA-2010-01; SR-EDGX- 2010-01; SR.... 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010-025). See also Securities Exchange...

  13. NOS-2 Inhibition in Phosgene-Induced Acute Lung Injury

    PubMed Central

    Filipczak, Piotr T.; Senft, Albert P.; Seagrave, JeanClare; Weber, Waylon; Kuehl, Philip J.; Fredenburgh, Laura E.; McDonald, Jacob D.; Baron, Rebecca M.

    2015-01-01

    Phosgene exposure via an industrial or warfare release produces severe acute lung injury (ALI) with high mortality, characterized by massive pulmonary edema, disruption of epithelial tight junctions, surfactant dysfunction, and oxidative stress. There are no targeted treatments for phosgene-induced ALI. Previous studies demonstrated that nitric oxide synthase 2 (NOS-2) is upregulated in the lungs after phosgene exposure; however, the role of NOS-2 in the pathogenesis of phosgene-induced ALI remains unknown. We previously demonstrated that NOS-2 expression in lung epithelium exacerbates inhaled endotoxin-induced ALI in mice, mediated partially through downregulation of surfactant protein B (SP-B) expression. Therefore, we hypothesized that a selective NOS-2 inhibitor delivered to the lung epithelium by inhalation would mitigate phosgene-induced ALI. Inhaled phosgene produced increases in bronchoalveolar lavage fluid protein, histologic lung injury, and lung NOS-2 expression at 24 h. Administration of the selective NOS-2 inhibitor 1400 W via inhalation, but not via systemic delivery, significantly attenuated phosgene-induced ALI and preserved epithelial barrier integrity. Furthermore, aerosolized 1400 W augmented expression of SP-B and prevented downregulation of tight junction protein zonula occludens 1 (ZO-1), both critical for maintenance of normal lung physiology and barrier integrity. We also demonstrate for the first time that NOS-2-derived nitric oxide downregulates the ZO-1 expression at the transcriptional level in human lung epithelial cells, providing a novel target for ameliorating vascular leak in ALI. Our data demonstrate that lung NOS-2 plays a critical role in the development of phosgene-induced ALI and suggest that aerosolized NOS-2 inhibitors offer a novel therapeutic strategy for its treatment. PMID:25870319

  14. Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle.

    PubMed

    Delgado, F; Estrada-Chávez, C; Romano, M; Paolicchi, F; Blanco-Viera, F; Capellino, F; Chavez-Gris, G; Pereira-Suárez, A L

    2010-09-01

    Paratuberculosis (PTB) is a chronic disease caused by M. avium subsp. paratuberculosis (MAP) that affects several animal species, and some studies have suggested that there may be a relationship between Crohn's disease and PTB. Significant aspects of PTB pathogenesis are not yet completely understood, such as the role of macrophages. Natural resistance-associated macrophage protein 1 (NRAMP1) and the inducible nitric oxide synthase (iNOS) molecules have shown nonspecific effects against several intracellular pathogens residing within macrophages. However, these molecules have been scarcely studied during natural infection with MAP. In this work, changes in NRAMP1 and iNOS expression were surveyed by immunohistochemistry in tissue samples from MAP-infected cattle and healthy controls. Our findings show strong specific immunolabeling against both NRAMP1 and iNOS molecules, throughout granulomatous PTB-compatible lesions in ileum and ileocaecal lymph nodes from paratuberculous cattle compared with uninfected controls, suggesting a relationship between the expression of these molecules and the pathogenesis of PTB disease. PMID:19345998

  15. JENDL Photonuclear Data File

    SciTech Connect

    Kishida, Norio; Murata, Toru; Asami, Tetsuo; Kosako, Kazuaki; Maki, Kouichi; Harada, Hideo; Lee, Young-Ouk; Chang, Jonghwa; Chiba, Satoshi; Fukahori, Tokio

    2005-05-24

    JENDL Photonuclear Data File 2004 was released in March 2004 and contains the photonuclear data for 68 nuclides from 2H to 237Np. We were proceeding on the evaluation work with the help of theoretical calculations based on statistical nuclear reaction models. The photonuclear cross sections that are to be contained in the file are as follows: photoabsorption cross sections, yield cross sections, and double-differential cross sections for photoneutrons, photoprotons, photodeuterons, phototritons, photo-3He-particles and photoalpha-particles, and isotope production cross sections. For the actinide nuclides, physical quantities related to photofission reactions are also included. The maximum energy of incident photons is 140 MeV, which is the energy at which the pion production channel opens.

  16. 77 FR 5493 - Marine Mammals; File No. 15802

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ... endangered and threatened species (50 CFR 222-226). On July 28, 2011 (76 FR 45230), notice was published that..., photographed, and released. On December 14, 2011 (76 FR 77781), notice was published clarifying the number of... National Oceanic and Atmospheric Administration RIN 0648-XA603 Marine Mammals; File No. 15802...

  17. 78 FR 42756 - Marine Mammals; File No. 14330

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-17

    ... endangered and threatened species (50 CFR 222-226). Permit No. 14330, issued on August 17, 2009 (74 FR 44822... (76 FR 329), to include authorization for harassment of additional Steller sea lions and harbor seals... National Oceanic and Atmospheric Administration RIN 0648-XA122 Marine Mammals; File No. 14330...

  18. 77 FR 26517 - Marine Mammals; File No. 14118

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-04

    ... Register (75 FR 13730) that a request for a permit to conduct research on the species identified above had... National Oceanic and Atmospheric Administration RIN 0648-XV40 Marine Mammals; File No. 14118 AGENCY... of the Marine Mammal Protection Act of 1972, as amended (16 U.S.C. 1361 et seq.), the...

  19. 75 FR 36064 - Marine Mammals; File No. 14186

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-24

    ... Register (73 FR 77630) that a request for a permit to conduct enhancement on the species identified above... National Oceanic and Atmospheric Administration RIN 0648-XM26 Marine Mammals; File No. 14186 AGENCY... Mammal Protection Act of 1972 as amended (MMPA; 16 U.S.C. 1361 et seq.). Permit No. 14186 authorizes...

  20. 77 FR 31585 - Marine Mammals; File No. 16388

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... National Oceanic and Atmospheric Administration RIN 0648-XB154 Marine Mammals; File No. 16388 AGENCY... Mammal Protection Act of 1972, as amended (MMPA; 16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as...

  1. 78 FR 21113 - Marine Mammals; File No. 17845

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XC599 Marine Mammals; File No. 17845 AGENCY... Mammal Protection Act of 1972, as amended (MMPA; 16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as...

  2. 77 FR 20793 - Marine Mammals; File No. 16599

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-06

    ... the Federal Register (77 FR 2512) that a request for a permit to conduct research on all species of... National Oceanic and Atmospheric Administration RIN 0648-XA905 Marine Mammals; File No. 16599 AGENCY... Dorian Houser, Ph.D., National Marine Mammal Foundation, 2240 Shelter Island Drive, 200, San Diego,...

  3. 76 FR 329 - Marine Mammals; File No. 14330

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-04

    ... endangered and threatened species (50 CFR parts 222-226). Permit No. 14330, issued on August 17, 2009 (74 FR... National Oceanic and Atmospheric Administration RIN 0648-XA122 Marine Mammals; File No. 14330 AGENCY... Marine Mammal Protection Act of 1972, as amended (16 U.S.C. 1361 et seq.), the regulations governing...

  4. 75 FR 76956 - Marine Mammals; File No. 15415

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-10

    ... National Oceanic and Atmospheric Administration RIN 0648-XA076 Marine Mammals; File No. 15415 AGENCY... Marine Mammal Protection Act of 1972, as amended (MMPA; 16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of...

  5. MERRA-2: File Specification

    NASA Technical Reports Server (NTRS)

    Bosilovich, M. G.; Lucchesi, R.; Suarez, M.

    2015-01-01

    The second Modern-Era Retrospective analysis for Research and Applications (MERRA-2) is a NASA atmospheric reanalysis that begins in 1980. It replaces the original MERRA reanalysis (Rienecker et al., 2011) using an upgraded version of the Goddard Earth Observing System Model, Version 5 (GEOS-5) data assimilation system. The file collections for MERRA-2 are described in detail in this document, including some important changes from those of the MERRA dataset (Lucchesi, 2012).

  6. The different potential of sponge bacterial symbionts in N₂ release indicated by the phylogenetic diversity and abundance analyses of denitrification genes, nirK and nosZ.

    PubMed

    Zhang, Xia; He, Liming; Zhang, Fengli; Sun, Wei; Li, Zhiyong

    2013-01-01

    Nitrogen cycle is a critical biogeochemical process of the oceans. The nitrogen fixation by sponge cyanobacteria was early observed. Until recently, sponges were found to be able to release nitrogen gas. However the gene-level evidence for the role of bacterial symbionts from different species sponges in nitrogen gas release is limited. And meanwhile, the quanitative analysis of nitrogen cycle-related genes of sponge microbial symbionts is relatively lacking. The nirK gene encoding nitrite reductase which catalyzes soluble nitrite into gas NO and nosZ gene encoding nitrous oxide reductase which catalyzes N₂O into N₂ are two key functional genes in the complete denitrification pathway. In this study, using nirK and nosZ genes as markers, the potential of bacterial symbionts in six species of sponges in the release of N2 was investigated by phylogenetic analysis and real-time qPCR. As a result, totally, 2 OTUs of nirK and 5 OTUs of nosZ genes were detected by gene library-based saturated sequencing. Difference phylogenetic diversity of nirK and nosZ genes were observed at OTU level in sponges. Meanwhile, real-time qPCR analysis showed that Xestospongia testudinaria had the highest abundance of nosZ gene, while Cinachyrella sp. had the greatest abundance of nirK gene. Phylogenetic analysis showed that the nirK and nosZ genes were probably of Alpha-, Beta-, and Gammaproteobacteria origin. The results from this study suggest that the denitrification potential of bacteria varies among sponges because of the different phylogenetic diversity and relative abundance of nosZ and nirK genes in sponges. Totally, both the qualitative and quantitative analyses of nirK and nosZ genes indicated the different potential of sponge bacterial symbionts in the release of nitrogen gas. PMID:23762300

  7. The effect of high protein diet and exercise on irisin, eNOS, and iNOS expressions in kidney.

    PubMed

    Tastekin, Ebru; Palabiyik, Orkide; Ulucam, Enis; Uzgur, Selda; Karaca, Aziz; Vardar, Selma Arzu; Yilmaz, Ali; Aydogdu, Nurettin

    2016-08-01

    Long-term effects of high protein diets (HPDs) on kidneys are still not sufficiently studied. Irisin which increases oxygen consumption and thermogenesis in white fat cells was shown in skeletal muscles and many tissues. Nitric oxide synthases (NOS) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. We aimed to investigate the effects of HPD, irisin and NO expression in kidney and relation of them with exercise and among themselves. Animals were grouped as control, exercise, HPD and exercise combined with HPD (exercise-HPD). Rats were kept on a HPD for 5 weeks and an exercise program was given them as 5 exercise and 2 rest days per week exercising on a treadmill with increasing speed and angle. In our study, while HPD group had similar total antioxidant capacity (TAC) levels with control group, exercise and exercise-HPD groups had lower levels (p < 0.05). Kidneys of exercising rats had no change in irisin or eNOS expression but their iNOS expression had increased (p < 0.001). HPD-E group has not been observed to cause kidney damage and not have a significant effect on rat kidney irisin, eNOS, or iNOS expression. Localization of irisin, eNOS, and iNOS staining in kidney is highly selective and quite clear in this study. Effects of exercise and HPD on kidney should be evaluated with different exercise protocols and contents of the diet. İrisin, eNOS, and iNOS staining localizations should be supported with various research studies. PMID:27277302

  8. Differential roles of iNOS and nNOS at rostral ventrolateral medulla during experimental endotoxemia in the rat.

    PubMed

    Chan, J Y; Wang, S H; Chan, S H

    2001-01-01

    We investigated the differential contribution of inducible and neuronal nitric oxide synthase (iNOS and nNOS) at the rostral ventrolateral medulla (RVLM) to endotoxemia induced by E. coli lipopolysaccharide (LPS). In Sprague-Dawley rats maintained under propofol anesthesia, i.v. administration of LPS (15, 30, or 45 mg/kg) induced a reduction (phase I), followed by an augmentation (phase II) and a secondary decrease (phase III) in the power density of the vasomotor components (0-0.8 Hz) in systemic arterial pressure (SAP) signals. LPS also induced an immediate hypotension, followed by a rebound increase and a secondary decrease in SAP. In addition, the level of iNOS mRNA exhibited a significant surge that began with phase I endotoxemia, reaching progressively its peak at phase III. Discernible down-regulation of nNOS mRNA was not detected until the last phase of endotoxemia. Pretreatment with microinjection of the selective iNOS inhibitor, aminoguanidine (250 pmol), into the bilateral RVLM significantly prolonged phases II and III endotoxemia, blunted the initial and secondary hypotension, and antagonized the upregulation of iNOS mRNA. Similar pretreatment with the selective nNOS inhibitor, 7-nitroindazole (1 pmol), on the other hand, discernibly shortened phase II and prolonged phase III endotoxemia, and induced progressive hypotension by antagonizing the rebound increase in SAP. We conclude that the relative prevalence of functional expression and molecular synthesis of iNOS over nNOS in the RVLM may be a crucial determinant for the reduction or loss in power density of the vasomotor components of SAP signals during experimental endotoxemia. PMID:11198360

  9. Ageing single file motion

    NASA Astrophysics Data System (ADS)

    Metzler, R.; Sanders, L.; Lomholt, M. A.; Lizana, L.; Fogelmark, K.; Ambjörnsson, Tobias

    2014-12-01

    The mean squared displacement of a tracer particle in a single file of identical particles with excluded volume interactions shows the famed Harris scaling ≃ K1/2t1/2 as function of time. Here we study what happens to this law when each particle of the single file interacts with the environment such that it is transiently immobilised for times τ with a power-law distribution ψ(τ) ≃ (τ★)α, and different ranges of the exponent α are considered. We find a dramatic slow-down of the motion of a tracer particle from Harris' law to an ultraslow, logarithmic time evolution ≃ K0 log 1/2(t) when 0 < α < 1. In the intermediate case 1 < α < 2, we observe a power-law form for the mean squared displacement, with a modified scaling exponent as compared to Harris' law. Once α is larger than two, the Brownian single file behaviour and thus Harris' law are restored. We also point out that this process is weakly non-ergodic in the sense that the time and ensemble averaged mean squared displacements are disparate.

  10. FRONT ELEVATIONS, NOS. 2122 AND 2124 ON THE WEST SIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FRONT ELEVATIONS, NOS. 2122 AND 2124 ON THE WEST SIDE OF UBER STREET, LOOKING WEST. - 2100 Block North Uber Street (Houses), East & west sides between Diamond Street & Susquehanna Avenue, Philadelphia, Philadelphia County, PA

  11. FRONT ELEVATIONS, NOS. 2123 AND 2125 ON THE EAST SIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FRONT ELEVATIONS, NOS. 2123 AND 2125 ON THE EAST SIDE OF UBER STREET, LOOKING EAST. - 2100 Block North Uber Street (Houses), East & west sides between Diamond Street & Susquehanna Avenue, Philadelphia, Philadelphia County, PA

  12. Los Angeles County Poor Farm, Patient Ward Nos. 210 & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Los Angeles County Poor Farm, Patient Ward Nos. 210 & 211 - Type B Plan, 7601 Imperial Highway; bounded by Esperanza Street, Laurel Street, Flores Street, and Descanso Street, Downey, Los Angeles County, CA

  13. Vault Area (original section), east corridor, looking north (Vault Nos. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Vault Area (original section), east corridor, looking north (Vault Nos. 1-9 - Fort McNair, Film Store House, Fort Lesley J. McNair, P Street between Third & Fourth Streets, Southwest, Washington, District of Columbia, DC

  14. Interior of Left Powerhouse showing generator Nos. 14. This view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior of Left Powerhouse showing generator Nos. 1-4. This view is from the catwalk at the level of the overhead crane, looking west. - Columbia Basin Project, Grand Coulee Dam Powerplant Complex, Grand Coulee, Grant County, WA

  15. 37. ISLAND PLANT: Nos. 1 AND 2 TWENTYSIX INCH SPECIAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    37. ISLAND PLANT: Nos. 1 AND 2 TWENTY-SIX INCH SPECIAL HORIZONTAL SAMSON TURBINE (RIVITED CASE) - American Falls Water, Power & Light Company, Island Power Plant, Snake River, below American Falls Dam, American Falls, Power County, ID

  16. 36. ISLAND PLANT: Nos. 1 AND 2 TWENTYSIX INCH HORIZONTAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. ISLAND PLANT: Nos. 1 AND 2 TWENTY-SIX INCH HORIZONTAL SAMSON TURBINES - American Falls Water, Power & Light Company, Island Power Plant, Snake River, below American Falls Dam, American Falls, Power County, ID

  17. 76 FR 76152 - City of Raleigh; Notice of Intent To File License Application, Filing of Pre-Application Document...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-06

    ... Marine Fisheries Service (NMFS) under section 7 of the Endangered Species Act and the joint agency... Conservation and Management Act and implementing regulations at 50 CFR 600.920; and (c) the North Carolina... Federal Energy Regulatory Commission City of Raleigh; Notice of Intent To File License Application,...

  18. Species Conservation and Management: Case Studies

    USGS Publications Warehouse

    Akcakaya, H.R.; Burgman, M.A.; Kindvall, O.; Wood, C.C.; Sjogren-Gulve, P.; Hatfield, J.S.; McCarthy, M.A.

    2004-01-01

    This edited volume is a collection of population and metapopulation models for a wide variety of species, including plants, invertebrates, fishes, amphibians, reptiles, birds, and mammals. Each chapter of the book describes the application of RAMAS GIS 4.0 to one species, with the aim of demonstrating how various life history characteristics of the species are incorporated into the model, and how the results of the model has been or can be used in conservation and management of the species. The book comes with a CD that includes a demo version of the program, and the data files for each species.

  19. 75 FR 74003 - Endangered Species; Permit No. 15677

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-30

    ... National Oceanic and Atmospheric Administration RIN 0648-XA060 Endangered Species; Permit No. 15677 AGENCY... under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and... Permits for Protected Species (APPS) home page, https:// ] apps.nmfs.noaa.gov/, and then selecting File...

  20. Investigating the Role of TNF-α and IFN-γ Activation on the Dynamics of iNOS Gene Expression in LPS Stimulated Macrophages

    PubMed Central

    Salim, Taha; Sershen, Cheryl L.; May, Elebeoba E.

    2016-01-01

    Macrophage produced inducible nitric oxide synthase (iNOS) is known to play a critical role in the proinflammatory response against intracellular pathogens by promoting the generation of bactericidal reactive nitrogen species. Robust and timely production of nitric oxide (NO) by iNOS and analogous production of reactive oxygen species are critical components of an effective immune response. In addition to pathogen associated lipopolysaccharides (LPS), iNOS gene expression is dependent on numerous proinflammatory cytokines in the cellular microenvironment of the macrophage, two of which include interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α). To understand the synergistic effect of IFN-γ and TNF-α activation, and LPS stimulation on iNOS expression dynamics and NO production, we developed a systems biology based mathematical model. Using our model, we investigated the impact of pre-infection cytokine exposure, or priming, on the system. We explored the essentiality of IFN-γ priming to the robustness of initial proinflammatory response with respect to the ability of macrophages to produce reactive species needed for pathogen clearance. Results from our theoretical studies indicated that IFN-γ and subsequent activation of IRF1 are essential in consequential production of iNOS upon LPS stimulation. We showed that IFN-γ priming at low concentrations greatly increases the effector response of macrophages against intracellular pathogens. Ultimately the model demonstrated that although TNF-α contributed towards a more rapid response time, measured as time to reach maximum iNOS production, IFN-γ stimulation was significantly more significant in terms of the maximum expression of iNOS and the concentration of NO produced. PMID:27276061

  1. eNOS-uncoupling in age-related erectile dysfunction

    PubMed Central

    Johnson, JM; Bivalacqua, TJ; Lagoda, GA; Burnett, AL; Musicki, B

    2011-01-01

    Aging is associated with ED. Although age-related ED is attributed largely to increased oxidative stress and endothelial dysfunction in the penis, the molecular mechanisms underlying this effect are not fully defined. We evaluated whether endothelial nitric oxide synthase (eNOS) uncoupling in the aged rat penis is a contributing mechanism. Correlatively, we evaluated the effect of replacement with eNOS cofactor tetrahydrobiopterin (BH4) on erectile function in the aged rats. Male Fischer 344 ‘young’ (4-month-old) and ‘aged’ (19-month-old) rats were treated with a BH4 precursor sepiapterin (10 mg/kg intraperitoneally) or vehicle for 4 days. After 1-day washout, erectile function was assessed in response to electrical stimulation of the cavernous nerve. Endothelial dysfunction (eNOS uncoupling) and oxidative stress (thiobarbituric acid reactive substances, TBARS) were measured by conducting western blot in penes samples. Erectile response was significantly reduced in aged rats, whereas eNOS uncoupling and TBARS production were significantly increased in the aged rat penis compared with young rats. Sepiapterin significantly improved erectile response in aged rats and prevented increase in TBARS production, but did not affect eNOS uncoupling in the penis of aged rats. These findings suggest that aging induces eNOS uncoupling in the penis, resulting in increased oxidative stress and ED. PMID:21289638

  2. The Goddard VLBI SINEX Files

    NASA Technical Reports Server (NTRS)

    Ma, Chopo; Petrov, Leonid

    2002-01-01

    The Goddard VLBI group generates SINEX files for individual 24-hr sessions and multiyear TRF solutions. Each 24-hr file includes the reduced normal matrix for the station positions, EOP, and EOP rates. A global Terrestrial Reference Frame (TRF) SINEX file has the reduced normal matrix for station positions and velocities but no EOP parameters. Both types of normal matrix are free from any datum constraints and are designed for combination solutions that will later apply the necessary datum constraints. The SINEX files also contain blocks that provide the constraints and covariance of well-defined 24-hr or global TRF solutions. These blocks are not yet part of the SINEX 2.0 specification. For the TRF solutions the Goddard VLBI group also generates the corresponding EOP time series. SINEX files and EOP time series are distributed through the IVS. The algorithms for generating the various blocks and the proper use of such SINEX files will be discussed.

  3. The Galley Parallel File System

    NASA Technical Reports Server (NTRS)

    Nieuwejaar, Nils; Kotz, David

    1996-01-01

    As the I/O needs of parallel scientific applications increase, file systems for multiprocessors are being designed to provide applications with parallel access to multiple disks. Many parallel file systems present applications with a conventional Unix-like interface that allows the application to access multiple disks transparently. The interface conceals the parallelism within the file system, which increases the ease of programmability, but makes it difficult or impossible for sophisticated programmers and libraries to use knowledge about their I/O needs to exploit that parallelism. Furthermore, most current parallel file systems are optimized for a different workload than they are being asked to support. We introduce Galley, a new parallel file system that is intended to efficiently support realistic parallel workloads. We discuss Galley's file structure and application interface, as well as an application that has been implemented using that interface.

  4. The Galley Parallel File System

    NASA Technical Reports Server (NTRS)

    Nieuwejaar, Nils; Kotz, David

    1996-01-01

    Most current multiprocessor file systems are designed to use multiple disks in parallel, using the high aggregate bandwidth to meet the growing I/0 requirements of parallel scientific applications. Many multiprocessor file systems provide applications with a conventional Unix-like interface, allowing the application to access multiple disks transparently. This interface conceals the parallelism within the file system, increasing the ease of programmability, but making it difficult or impossible for sophisticated programmers and libraries to use knowledge about their I/O needs to exploit that parallelism. In addition to providing an insufficient interface, most current multiprocessor file systems are optimized for a different workload than they are being asked to support. We introduce Galley, a new parallel file system that is intended to efficiently support realistic scientific multiprocessor workloads. We discuss Galley's file structure and application interface, as well as the performance advantages offered by that interface.

  5. 12 CFR 5.4 - Filing required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CORPORATE ACTIVITIES Rules of General Applicability § 5.4 Filing required. (a) Filing. A depository institution shall file an application or notice with the OCC to engage in corporate activities and... advise an applicant through a pre-filing communication to send the filing or submission directly to...

  6. 76 FR 61685 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... Supply Corporation submits tariff filing per 154.204: Rate Schedule PTR to be effective 10/27/2011. Filed... Negotiated Rate Agreement Filing to be effective 10/1/2011. Filed Date: 09/28/2011. Accession Number... effective 10/1/2011. Filed Date: 09/28/2011. Accession Number: 20110928-5034. Comment Date: 5 p.m....

  7. 78 FR 16491 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    .... Description: Housekeeping tariff filing to be effective 4/4/2013. Filed Date: 3/5/13. Accession Number... Numbers: RP13-673-000. Applicants: B-R Pipeline Company. Description: Housekeeping tariff filing to be... Housekeeping Filing to be effective 4/8/2013. Filed Date: 3/6/13. Accession Number: 20130306-5067. Comments...

  8. 75 FR 61730 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ... Energy Regulatory Commission Combined Notice of Filings September 21, 2010. Take notice that the... filing per 154.203: Cotton Valley--Baseline eTariff Filing to be effective 9/21/2010. Filed Date: 09/21... . To facilitate electronic service, persons with Internet access who will eFile a document and/or...

  9. 29 CFR 2200.8 - Filing.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... document shall be filed. (e) Filing date. (1) Except for the documents listed in paragraph (e)(2) of this... 29 Labor 9 2010-07-01 2010-07-01 false Filing. 2200.8 Section 2200.8 Labor Regulations Relating to... § 2200.8 Filing. (a) What to file. All papers required to be served on a party or intervenor, except...

  10. 43 CFR 4.1362 - Where to file; when to file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Where to file; when to file. 4.1362... file; when to file. (a) The request for review shall be filed with the Hearings Division, Office of... bear the expense for this service. (b) Failure to file a request for review within the time...

  11. Converting Inhouse Subject Card Files to Electronic Keyword Files.

    ERIC Educational Resources Information Center

    Culmer, Carita M.

    The library at Phoenix College developed the Controversial Issues Files (CIF), a "home made" card file containing references pertinent to specific ongoing assignments. Although the CIF had proven itself to be an excellent resource tool for beginning researchers, it was cumbersome to maintain in the card format, and was limited to very general…

  12. The DNA Files

    SciTech Connect

    1998-06-09

    The DNA Files is a radio documentary which disseminates genetics information over public radio. The documentaries explore subjects which include the following: How genetics affects society. How human life began and how it evolved. Could new prenatal genetic tests hold the key to disease prevention later in life? Would a national genetic data base sacrifice individual privacy? and Should genes that may lead to the cure for cancer be privately owned? This report serves as a project update for the second quarter of 1998. It includes the spring/summer 1998 newsletter, the winter 1998 newsletter, the program clock, and the latest flyer.

  13. Muscular nitric oxide synthase (muNOS) and utrophin.

    PubMed

    Chaubourt, Emmanuel; Voisin, Vincent; Fossier, Philippe; Baux, Gérard; Israël, Maurice; De La Porte, Sabine

    2002-01-01

    Duchenne muscular dystrophy (DMD), the severe X-linked recessive disorder which results in progressive muscle degeneration, is due to a lack of dystrophin, a membrane cytoskeletal protein. Three types of treatment are envisaged: pharmacological (glucocorticoid), myoblast transplantation, and gene therapy. An alternative to the pharmacological approach is to compensate for dystrophin loss by the upregulation of another cytoskeletal protein, utrophin. Utrophin and dystrophin are part of a complex of proteins and glycoproteins, which links the basal lamina to the cytoskeleton, thus ensuring the stability of the muscle membrane. One protein of the complex, syntrophin, is associated with a muscular isoform of the neuronal nitric oxide synthase (nNOS). We have demonstrated an overexpression of utrophin, visualised by immunofluorescence and quantified by Western blotting, in normal myotubes and in mdx (the animal model of DMD) myotubes, as in normal (C57) and mdx mice, both treated with nitric oxide (NO) donor or L-arginine, the NOS substrate. There is evidence that utrophin may be capable of performing the same cellular functions as dystrophin and may functionally compensate for its lack. Thus, we propose to use NO donors, as palliative treatment of Duchenne and Becker muscular dystrophies, pending, or in combination with, gene and/or cellular therapy. Discussion has focussed on the various isoforms of NOS that could be implicated in the regeneration process. Dystrophic and healthy muscles respond to treatment, suggesting that although NOS is delocalised in the cytoplasm in the case of DMD, it conserves substantial activity. eNOS present in mitochondria and iNOS present in cytoplasm and the neuromuscular junction could also be activated. Lastly, production of NO by endothelial NOS of the capillaries would also be beneficial through increased supply of metabolites and oxygen to the muscles. PMID:11755782

  14. 78 FR 69408 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-19

    ... Pipeline LLC. Description: Northwest Pipeline LLC submits tariff filing per 154.204: NWP 2013 Housekeeping...: National Grid LNG, LLC submits tariff filing per 154.204: Housekeeping Filing to be effective...

  15. 78 FR 76293 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-17

    ...: RP14-274-000. Applicants: NGO Transmission, Inc. Description: NGO Transmission, Inc. submits tariff filing per 154.204: NGO Transmission, Inc.--Negotiated Rate Filing to be effective 1/1/2014. Filed...

  16. 76 FR 39863 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ..., July 11, 2011. Docket Numbers: RP11-2219-000. Applicants: Gulf South Pipeline Company, LP. Description... Negotiated Rate Agreement Filing to be effective 7/1/2011 under RP11-2219 Filing Type: 570 Filed Date:...

  17. 75 FR 67357 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-02

    .... Applicants: ETC Tiger Pipeline, LLC. Description: ETC Tiger Pipeline, LLC submits tariff filing per 154.203: Tiger FTSA Non-Conforming Agreement Filing to be effective 12/ 1/2010. Filed Date: 10/25/2010....

  18. 78 FR 65636 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-01

    ...: Cheniere Creole Trail Pipeline, L.P. Description: Compliance Filing to be effective 11/1/2013. Filed Date... Supply Corporation. Description: Amendment-Appalachian Pooling Filing (RP13-1348) to be effective...

  19. Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus).

    PubMed

    Yadav, Vijay Pratap; Dangi, Satyaveer Singh; Chouhan, Vikrant Singh; Gupta, Mahesh; Dangi, Saroj K; Singh, Gyanendra; Maurya, Vijay Prakash; Kumar, Puneet; Sarkar, Mihir

    2016-03-01

    Approximately 50 genes other than heat shock protein (HSP) expression changes during thermal stress. These genes like nitric oxide synthase (NOS) need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals. So, the present study was undertaken to demonstrate the expressions of inducible form type II NOS (iNOS), endothelial type III NOS (eNOS), constitutively expressed enzyme NOS (cNOS), HSP70, and HSP90 in peripheral blood mononuclear cells (PBMCs) during different seasons in Barbari goats. Real-time polymerase chain reaction, western blot, and immunocytochemistry were applied to investigate messenger RNA (mRNA) expression, protein expression, and immunolocalization of examined factors. The mRNA and protein expressions of iNOS, eNOS, cNOS, HSP70, and HSP90 were significantly higher (P < 0.05) during peak summer, and iNOS and eNOS expressions were also observed to be significantly higher (P < 0.05) during peak winter season as compared with moderate season. The iNOS, eNOS, cNOS, HSP70, and HSP90 were mainly localized in plasma membrane and cytoplasm of PBMCs. To conclude, data generated in the present study indicate the possible involvement of the NOS family genes in amelioration of thermal stress so as to maintain cellular integrity and homeostasis in goats. PMID:26205811

  20. Pedagogical Reflections by Secondary Science Teachers at Different NOS Implementation Levels

    NASA Astrophysics Data System (ADS)

    Herman, Benjamin C.; Clough, Michael P.; Olson, Joanne K.

    2015-10-01

    This study investigated what 13 secondary science teachers at various nature of science (NOS) instruction implementation levels talked about when they reflected on their teaching. We then determined if differences exist in the quality of those reflections between high, medium, and low NOS implementers. This study sought to answer the following questions: (1) What do teachers talk about when asked general questions about their pedagogy and NOS pedagogy and (2) what qualitative differences, if any, exist within variables across teachers of varying NOS implementation levels? Evidence derived from these teachers' reflections indicated that self-efficacy and perceptions of general importance for NOS instruction were poor indicators of NOS implementation. However, several factors were associated with the extent that these teachers implemented NOS instruction, including the utility value they hold for NOS teaching, considerations of how people learn, understanding of NOS pedagogy, and their ability to accurately and deeply self-reflect about teaching. Notably, those teachers who effectively implemented the NOS at higher levels value NOS instruction for reasons that transcend immediate instructional objectives. That is, they value teaching NOS for achieving compelling ends realized long after formal schooling (e.g., lifelong socioscientific decision-making for civic reasons), and they deeply reflect about how to teach NOS by drawing from research about how people learn. Low NOS implementers' simplistic notions and reflections about teaching and learning appeared to be impeding factors to accurate and consistent NOS implementation. This study has implications for science teacher education efforts that promote NOS instruction.

  1. Expression analysis of NOS family and HSP genes during thermal stress in goat ( Capra hircus)

    NASA Astrophysics Data System (ADS)

    Yadav, Vijay Pratap; Dangi, Satyaveer Singh; Chouhan, Vikrant Singh; Gupta, Mahesh; Dangi, Saroj K.; Singh, Gyanendra; Maurya, Vijay Prakash; Kumar, Puneet; Sarkar, Mihir

    2016-03-01

    Approximately 50 genes other than heat shock protein (HSP) expression changes during thermal stress. These genes like nitric oxide synthase (NOS) need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals. So, the present study was undertaken to demonstrate the expressions of inducible form type II NOS (iNOS), endothelial type III NOS (eNOS), constitutively expressed enzyme NOS (cNOS), HSP70, and HSP90 in peripheral blood mononuclear cells (PBMCs) during different seasons in Barbari goats. Real-time polymerase chain reaction, western blot, and immunocytochemistry were applied to investigate messenger RNA (mRNA) expression, protein expression, and immunolocalization of examined factors. The mRNA and protein expressions of iNOS, eNOS, cNOS, HSP70, and HSP90 were significantly higher ( P < 0.05) during peak summer, and iNOS and eNOS expressions were also observed to be significantly higher ( P < 0.05) during peak winter season as compared with moderate season. The iNOS, eNOS, cNOS, HSP70, and HSP90 were mainly localized in plasma membrane and cytoplasm of PBMCs. To conclude, data generated in the present study indicate the possible involvement of the NOS family genes in amelioration of thermal stress so as to maintain cellular integrity and homeostasis in goats.

  2. TIGER/LINE FILES, 1998

    EPA Science Inventory

    TIGER, TIGER/Line, and Census TIGER are registered trademarks of the Bureau of the Census. The 1998 TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER data base. The geographic coverage for a single TIGER/Line file is a cou...

  3. Portable File Format (PFF) specifications.

    SciTech Connect

    Dolan, Daniel H.,

    2015-02-01

    Created at Sandia National Laboratories, the Portable File Format (PFF) allows binary data transfer across computer platforms. Although this capability is supported by many other formats, PFF files are still in use at Sandia, particularly in pulsed power research. This report provides detailed PFF specifications for accessing data without relying on legacy code.

  4. Aerospell Supplemental Spell Check File

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Aerospell is a supplemental spell check file that can be used as a resource for researchers, writers, editors, students, and others who compose scientific and technical texts. The file extends the general spell check dictionaries of word processors by adding more than 13,000 words used in a broad range of aerospace and related disciplines.

  5. Viewing Files | Smokefree.gov

    Cancer.gov

    In addition to standard HTML Web pages, our Web site contains files in other formats. You may need additional software or browser plug-ins to view some of these files. The following list shows each format along with links to the corresponding freely available plug-ins or viewers.

  6. Melanoma NOS1 expression promotes dysfunctional IFN signaling

    PubMed Central

    Liu, Qiuzhen; Tomei, Sara; Ascierto, Maria Libera; De Giorgi, Valeria; Bedognetti, Davide; Dai, Cuilian; Uccellini, Lorenzo; Spivey, Tara; Pos, Zoltan; Thomas, Jaime; Reinboth, Jennifer; Murtas, Daniela; Zhang, Qianbing; Chouchane, Lotfi; Weiss, Geoffrey R.; Slingluff, Craig L.; Lee, Peter P.; Rosenberg, Steven A.; Alter, Harvey; Yao, Kaitai; Wang, Ena; Marincola, Francesco M.

    2014-01-01

    In multiple forms of cancer, constitutive activation of type I IFN signaling is a critical consequence of immune surveillance against cancer; however, PBMCs isolated from cancer patients exhibit depressed STAT1 phosphorylation in response to IFN-α, suggesting IFN signaling dysfunction. Here, we demonstrated in a coculture system that melanoma cells differentially impairs the IFN-α response in PBMCs and that the inhibitory potential of a particular melanoma cell correlates with NOS1 expression. Comparison of gene transcription and array comparative genomic hybridization (aCGH) between melanoma cells from different patients indicated that suppression of IFN-α signaling correlates with an amplification of the NOS1 locus within segment 12q22-24. Evaluation of NOS1 levels in melanomas and IFN responsiveness of purified PBMCs from patients indicated a negative correlation between NOS1 expression in melanomas and the responsiveness of PBMCs to IFN-α. Furthermore, in an explorative study, NOS1 expression in melanoma metastases was negatively associated with patient response to adoptive T cell therapy. This study provides a link between cancer cell phenotype and IFN signal dysfunction in circulating immune cells. PMID:24691438

  7. Modulating DDAH/NOS Pathway to Discover Vasoprotective Insulin Sensitizers

    PubMed Central

    Lai, Li; Ghebremariam, Yohannes T.

    2016-01-01

    Insulin resistance syndrome (IRS) is a configuration of cardiovascular risk factors involved in the development of metabolic disorders including type 2 diabetes mellitus. In addition to diet, age, socioeconomic, and environmental factors, genetic factors that impair insulin signaling are centrally involved in the development and exacerbation of IRS. Genetic and pharmacological studies have demonstrated that the nitric oxide (NO) synthase (NOS) genes are critically involved in the regulation of insulin-mediated glucose disposal. The generation of NO by the NOS enzymes is known to contribute to vascular homeostasis including insulin-mediated skeletal muscle vasodilation and insulin sensitivity. By contrast, excessive inhibition of NOS enzymes by exogenous or endogenous factors is associated with insulin resistance (IR). Asymmetric dimethylarginine (ADMA) is an endogenous molecule that competitively inhibits all the NOS enzymes and contributes to metabolic perturbations including IR. The concentration of ADMA in plasma and tissue is enzymatically regulated by dimethylarginine dimethylaminohydrolase (DDAH), a widely expressed enzyme in the cardiovascular system. In preclinical studies, overexpression of DDAH has been shown to reduce ADMA levels, improve vascular compliance, and increase insulin sensitivity. This review discusses the feasibility of the NOS/DDAH pathway as a novel target to develop vasoprotective insulin sensitizers. PMID:26770984

  8. Gamma-decanolactone inhibits iNOS and TNF-alpha production by lipopolysaccharide-activated microglia in N9 cells.

    PubMed

    Pflüger, Pricila; Viau, Cassiana Macagnan; Coelho, Vanessa Rodrigues; Berwig, Natália Alice; Staub, Renata Bartolomeu; Pereira, Patrícia; Saffi, Jenifer

    2016-06-01

    Activated microglia that produce reactive nitrogen species (RNS), inflammatory factors, reactive oxygen species (ROS), and other neurovirulent factors may lead to the development of neurodegenerative diseases. Certain compounds can inhibit the activation of microglia. However, these mechanisms remain unclear. In the present study, we investigated the inhibitory effect of Gamma-decanolactone (GD) on the production of reactive oxygen species and inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS) - stimulated N9 murine microglial cells through the p38 MAPK signaling pathway. The results showed that GD attenuated the activation of N9 cells and inhibited intracellular reactive oxygen species and the expression of iNOS and TNF-α induced by LPS in the cells. In addition, GD blocked the phosphorylation of p38 and inhibited cleaved caspase-9 and DNA damage. These data indicate that GD has therapeutic potential for the treatment of neurodegenerative diseases, and that it exerts its effects by inhibiting inflammation. PMID:27012990

  9. Roles of ROS and PKC-βII in ionizing radiation-induced eNOS activation in human vascular endothelial cells.

    PubMed

    Sakata, Kimimasa; Kondo, Takashi; Mizuno, Natsumi; Shoji, Miki; Yasui, Hironobu; Yamamori, Tohru; Inanami, Osamu; Yokoo, Hiroki; Yoshimura, Naoki; Hattori, Yuichi

    2015-07-01

    Vascular endothelial cells can absorb higher radiation doses than any other tissue in the body, and post-radiation impaired endothelial nitric oxide synthase (eNOS) function may be developed as a potential contributor to the pathogenesis of vascular injury. In this study, we investigated early alterations of eNOS signaling in human umbilical venous endothelial cells (HUVECs) exposed to X-ray radiation. We found that ionizing radiation increased eNOS phosphorylation at Ser-1177 and dephosphorylation at Thr-495 in HUVECs in a dose-dependent (≤ 20 Gy) and time-dependent (6-72 h) manner. The total expression levels of eNOS were unchanged by radiation. Although a transient but significant increase in extracellular signal-regulated protein kinase 1/2 (ERK1/2) phosphorylation and a biphasic decline in Akt phosphorylation were observed after irradiation, these inhibitors were without effect on the radiation-induced changes in eNOS phosphorylation. There was an increase in protein kinase C-βII (PKC-βII) expression and the ablation of PKC-βII by small interfering RNA (siRNA) negated the radiation effect on the two eNOS phosphorylation events. Furthermore, when the radiation-induced increase in reactive oxygen species (ROS) generation was prevented by the anti-oxidant N-acetyl-L-cysteine, eNOS Ser-1177 phosphorylation and Thr-495 dephosphorylation in irradiated HUVECs were significantly reduced. However, transfection of PKC-β siRNA did not alter ROS production after irradiation, and NAC failed to block the radiation-induced increase in PKC-βII expression. Taken together, our results suggest that ionizing radiation-induced eNOS activation in human vascular endothelial cells is attributed to both the up-regulation of PKC-βII and the increase in ROS generation which were independent of each other. PMID:25869503

  10. Adenoviral gene transfer of endothelial nitric-oxide synthase (eNOS) partially restores normal pulmonary arterial pressure in eNOS-deficient mice

    PubMed Central

    Champion, Hunter C.; Bivalacqua, Trinity J.; Greenberg, Stanley S.; Giles, Thomas D.; Hyman, Albert L.; Kadowitz, Philip J.

    2002-01-01

    It has been shown that mice deficient in the gene coding for endothelial nitric-oxide synthase (eNOS) have increased pulmonary arterial pressure and pulmonary vascular resistance. In the present study, the effect of transfer to the lung of an adenoviral vector encoding the eNOS gene (AdCMVeNOS) on pulmonary arterial pressure and pulmonary vascular resistance was investigated in eNOS-deficient mice. One day after intratracheal administration of AdCMVeNOS to eNOS−/− mice, there was an increase in eNOS protein, cGMP levels, and calcium-dependent conversion of l-arginine to l-citrulline in the lung. The increase in eNOS protein and activity in eNOS−/− mice was associated with a reduction in mean pulmonary arterial pressure and pulmonary vascular resistance when compared with values in eNOS-deficient mice treated with vehicle or a control adenoviral vector coding for β-galactosidase, AdCMVβgal. These data suggest that in vivo gene transfer of eNOS to the lung in eNOS−/− mice can increase eNOS staining, eNOS protein, calcium-dependent NOS activity, and cGMP levels and partially restore pulmonary arterial pressure and pulmonary vascular resistance to near levels measured in eNOS+/+ mice. Thus, the major finding in this study is that in vivo gene transfer of eNOS to the lung in large part corrects a genetic deficiency resulting from eNOS deletion and may be a useful therapeutic intervention for the treatment of pulmonary hypertensive disorders in which eNOS activity is reduced. PMID:12237402

  11. Storing files in a parallel computing system using list-based index to identify replica files

    DOEpatents

    Faibish, Sorin; Bent, John M.; Tzelnic, Percy; Zhang, Zhenhua; Grider, Gary

    2015-07-21

    Improved techniques are provided for storing files in a parallel computing system using a list-based index to identify file replicas. A file and at least one replica of the file are stored in one or more storage nodes of the parallel computing system. An index for the file comprises at least one list comprising a pointer to a storage location of the file and a storage location of the at least one replica of the file. The file comprises one or more of a complete file and one or more sub-files. The index may also comprise a checksum value for one or more of the file and the replica(s) of the file. The checksum value can be evaluated to validate the file and/or the file replica(s). A query can be processed using the list.

  12. Particle Physics Masterclass as a Context for Learning about NOS

    NASA Astrophysics Data System (ADS)

    Wadness, Michael

    2011-04-01

    This research addresses the question: Do secondary school science students attending the U.S. Particle Physics Masterclass change their view of the nature of science (NOS)? The U.S. Particle Physics Masterclass is a national physics outreach program run by QuarkNet, in which high school physics students gather at a local research institution for one day to learn about particle physics and the scientific enterprise. Student activities include introductory lectures in particle physics, laboratory tours, analysis of actual data from CERN, and the discussion of their findings in a conference-like atmosphere. Although there are a number of outreach programs involving scientists in K-12 education, very few of them have been formally evaluated to determine if they provide adequate learning of NOS. Therefore, the significance of this study is that it investigates the claim that science outreach programs may be designed to address science literacy, specifically as a context for explicit NOS instruction.

  13. 78 FR 17394 - Filing via the Internet; Electronic Tariff Filings; Revisions to Electric Quarterly Report Filing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-21

    .... 770, 77 FR 71288 (Nov. 30, 2012), FERC Stats. & Regs. ] 31,338 (cross-referenced at 141 FERC ] 61,120... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Filing via the Internet; Electronic Tariff Filings; Revisions to...

  14. 76 FR 20324 - Endangered Species; File No. 15606

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... INFORMATION: On November 24, 2010, notice was published in the Federal Register (75 FR 71670) that a request... University at Galveston, Department of Marine Biology, 5007 Avenue U, Galveston, TX 77553, has been issued...

  15. 78 FR 57353 - Endangered Species; File No. 14726

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-18

    ...Notice is hereby given that Blair Witherington, Ph.D., Florida Fish and Wildlife Conservation Commission, 9700 South A1A, Melbourne Beach, FL, 32951, has requested a modification to scientific research Permit No....

  16. 78 FR 44096 - Endangered Species; File No. 17381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    ..., notice was published in the Federal Register (77 FR 72326) that a request for a scientific research.... ACTION: Issuance of permit. SUMMARY: Notice is hereby given that Kristen Hart, Ph.D., United...

  17. 77 FR 34349 - Endangered Species; File No. 16803

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ...Notice is hereby given that NMFS Southwest Fisheries Science Center (SWFSC), 3333 N. Torrey Pines Ct., La Jolla, CA 92037, [Responsible Party: Lisa Ballance, Ph.D.], has applied in due form for a permit to take green (Chelonia mydas), loggerhead (Caretta caretta), and olive ridley (Lepidochelys olivacea) sea turtles for scientific...

  18. 78 FR 16255 - Endangered Species; File No. 17022

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-14

    ... (77 FR 37877) that a request for a scientific research permit to take green and hawksbill sea turtles... Science Center (PIFSC; Samuel Pooley, Ph.D., Responsible Party), has been issued a permit to take...

  19. 77 FR 30261 - Endangered Species; File No. 16306

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-22

    ..., 2011, notice was published in the Federal Register (76 FR 58471) that a request for a scientific..., (Gail Wippelhauser, Ph.D., Responsible Party), 21 State House Station, Augusta, ME 04333, has...

  20. 77 FR 1062 - Endangered Species; File No. 16146

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-09

    ... Federal Register (76 FR 44306) that a request for a scientific research permit to take loggerhead, green.... ACTION: Issuance of permit. SUMMARY: Notice is hereby given that Kristen Hart, Ph.D., U.S....

  1. 78 FR 15706 - Endangered Species; File No. 17316

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... INFORMATION: On September 24, 2012, notice was published in the Federal Register (77 FR 58812) that a request.... ACTION: Issuance of permit. SUMMARY: Notice is hereby given that George Burgess, Ph.D., Florida Museum...

  2. 76 FR 71515 - Endangered Species; File No. 16174

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ..., 2011, notice was published in the Federal Register (76 FR 18725) that a request for a scientific.... ACTION: Issuance of permit. SUMMARY: Notice is hereby given that Michael Salmon, Ph.D., Florida...

  3. 78 FR 17359 - Endangered Species; File No. 17095-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-21

    ...: On January 29, 2013, notice was published in the Federal Register (78 FR 6072) that a request for a... integrated transponder and dart tags, photograph, tissue sample, and release up to 82 shortnose sturgeon...

  4. 75 FR 6184 - Endangered Species; File No. 14754

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ...Notice is hereby given that Isaac Wirgin, PhD, New York University School of Medicine, Department of Environmental Medicine, Tuxedo, NY 10987, has applied in due form for a permit to import and take shortnose sturgeon (Acipenser brevirostrum) early life stages (ELS) for purposes of scientific...

  5. 75 FR 19363 - Endangered Species; File No. 14754

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ...Notice is hereby given that Isaac Wirgin, PhD, New York University School of Medicine, Department of Environmental Medicine, Tuxedo, NY 10987, has been issued a permit to take shortnose sturgeon (Acipenser brevirostrum) for purposes of scientific...

  6. 76 FR 4635 - Endangered Species; File No. 15552

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ...), loggerhead (Caretta caretta), Kemp's ridley (Lepidochelys kempii), olive ridley (Lepidochelys olivacea..., loggerhead, hawksbill, leatherback, Kemp's ridley, olive ridley, and unidentified hardshell sea turtles by... 120 olive ridley/ unknown hardshell sea turtles would be handled, identified, photographed,...

  7. 77 FR 15997 - Endangered Species; File No. 15672

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-19

    ..., notice was published in the Federal Register (76 FR 23305) that a request for a scientific research... have been disentangled from fishing gear by a stranding network or captured with a breakaway...

  8. 77 FR 73024 - Endangered Species; File No. 16547-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-07

    ... Permit No. 16547, issued on April 11, 2012 (77 FR 21754), is requested under the authority of the... parts per thousand salinity. Researchers are currently authorized to capture adult, juvenile and early..., sub-adult and juvenile) and the salinity level where captured; or ] also externally satellite...

  9. 78 FR 19217 - Endangered Species; File No. 16547-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    .... SUPPLEMENTARY INFORMATION: On December 7, 2012, notice was published in the Federal Register (77 FR 73024) that... above and below 22 parts per thousand salinity. Researchers are currently authorized to capture adult... telemetry tags dependent on the life stage (adult, sub-adult and juvenile) and the salinity level...

  10. 78 FR 14078 - Endangered Species; File No. 17405

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-04

    ...Notice is hereby given that David Lapota, Ph.D., Department of the Navy, SPAWAR Systems Center, Pacific, Environmental Sciences Division, 53475 Strothe Road, San Diego, CA 92152 has applied in due form for a permit to take black abalone (Haliotis cracherodii) for purposes of scientific...

  11. 78 FR 32623 - Endangered Species; File No. 17452

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ...Notice is hereby given that Caleb Slater, Massachusetts Division of Fish and Wildlife, 1 Rabbit Hill Road, Westborough, MA, 01581, has applied in due form for a permit to take shortnose (Acipenser brevirostrum) and Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) for purposes of scientific...

  12. 76 FR 78890 - Endangered Species; File No. 15566

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    ... to Permit No. 15566, issued on April 8, 2011 (76 FR 22877), is requested under the authority of the... in order to assess temporal change in catch rates, size distributions, sex and genetic ratios,...

  13. 77 FR 27718 - Endangered Species; File No. 15566

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... published in the Federal Register (76 FR 78890) that a modification of Permit No. 15566 had been requested... No. 15566, issued April 8, 2011 (76 FR 22877), authorizes capture by trawl of 345 loggerhead (Caretta..., size distributions, sex and genetic ratios, and health of sea turtles. Captures occur annually...

  14. 77 FR 54566 - Endangered Species; File No. 16134

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    .... SUPPLEMENTARY INFORMATION: On December 9, 2011, notice was published in the Federal Register (76 FR 76950) that... distribution, sex ratio, health parameters and genetic diversity of loggerhead and Kemp's ridley sea turtles...

  15. 75 FR 71670 - Endangered Species; File No. 15606

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-24

    ...&M University at Galveston, Department of Marine Biology, 5007 Avenue U, Galveston, TX 77553, has... satellite transmitters to and release sea turtles. In project 1, the applicant proposes to take up to 150... to release. In project 2, the applicant proposes to take up to 15 green, 135 Kemp's ridley, and...

  16. 75 FR 26715 - Endangered Species; File No. 10022

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... INFORMATION: On September 30, 2009, notice was published in the Federal Register (74 FR 50172) that a modification of Permit No. 10022, issued April 23, 2008 (73 FR 23195), had been requested by the above-named..., Department of Wildlife Ecology and Conservation, University of Florida, P.O. Box 110485, Gainesville,...

  17. 78 FR 39258 - Endangered Species; File No. 18069

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-01

    ...Notice is hereby given that Jeffrey Schmid, Ph.D., Conservancy of Southwest Florida, 1450 Merrihue Drive, Naples, FL 34102, has applied in due form for a permit to take Kemp's ridley (Lepidochelys kempii), loggerhead (Caretta caretta), green (Chelonia mydas), and hawksbill (Eretmochelys imbricata) sea turtles for purposes of scientific...

  18. 77 FR 65673 - Endangered Species; File No. 16248

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ...Notice is hereby given that the Riverbanks Zoo and Garden, P.O. Box 1060, Columbia, South Carolina 29202 [Jennifer Rawlings, Responsible Party], has applied in due form for a permit to hold shortnose sturgeon (Acipenser brevirostrum) for the purposes of...

  19. 77 FR 72326 - Endangered Species; File No. 17381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ...Notice is hereby given that Kristen Hart, Ph.D., United States Geological Survey, Southeast Ecological Science Center, 3205 College Avenue, Davie, FL 33314, has applied in due form for a permit to take green (Chelonia mydas), loggerhead (Caretta caretta), hawksbill (Eretmochelys imbricata), and Kemp's ridley (Lepidochelys kempii) sea turtles for the purposes of scientific...

  20. 78 FR 17355 - Endangered Species; File No. 17787

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-21

    ...Notice is hereby given that the NMFS Southeast Fisheries Center (SEFSC); 75 Virginia Beach Drive, Miami, FL 33149 [Responsible Party: Bonnie Ponwith, Ph.D.], has applied in due form for a permit to take smalltooth sawfish (Pristis pectinata) for purposes of scientific...

  1. 76 FR 45517 - Endangered Species; File No. 13330-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-29

    ... in the Federal Register (76 FR 14650) that a scientific research permit modification to take... replace plastic rototags used to secure VEMCO acoustic transmitters with neoprene clasp tags; and...

  2. 75 FR 4043 - Endangered Species; File No. 14396

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-26

    ...Notice is hereby given the Delaware Department of Natural Resources and Environmental Control-Division of Fish and Wildlife, Dover, DE, has been issued a permit to take shortnose sturgeon (Acipenser brevirostrum) for purposes of scientific...

  3. 78 FR 6072 - Endangered Species; File No. 17095-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ...Notice is hereby given that Entergy Nuclear Operations Inc., 450 Broadway, Suite 3, Buchanan, NY 10511, has requested a modification to scientific research Permit No. 17095 authorizing scientific research on endangered shortnose sturgeon (Acipenser brevirostrum) and Atlantic sturgeon (Acipenser oxyrinchus...

  4. 76 FR 31597 - Endangered Species; File No. 15614

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    ...Notice is hereby given that Tom Savoy, Connecticut Department of Environmental Protection, Marine Fisheries, PO Box 719, Old Lyme, CT 06731, has been issued a permit to take shortnose sturgeon for purposes of scientific...

  5. 76 FR 33245 - Endangered Species; File No. 15135

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... Register (75 FR 11863) that a request for a scientific research permit to take sea turtles had been... scientific research. ADDRESSES: The permit and related documents are available for review upon written... (Paralichthys lethostigma) in shallow waters of Core Sound, North Carolina. The objective of the research is...

  6. Efficient algorithms for multi-file caching

    SciTech Connect

    Otoo, Ekow J.; Rotem, Doron; Seshadri, Sridhar

    2004-03-15

    Multi-File Caching issues arise in applications where a set of jobs are processed and each job requests one or more input files. A given job can only be started if all its input files are preloaded into a disk cache. Examples of applications where Multi-File caching may be required are scientific data mining, bit-sliced indexes, and analysis of sets of vertically partitioned files. The difference between this type of caching and traditional file caching systems is that in this environment, caching and replacement decisions are made based on ''combinations of files (file bundles),'' rather than single files. In this work we propose new algorithms for Multi-File caching and analyze their performance. Extensive simulations are presented to establish the effectiveness of the Multi-File caching algorithm in terms of job response time and job queue length.

  7. Exploring Elementary Science Methods Course Contexts to Improve Preservice Teachers' NOS of Science Conceptions and Understandings of NOS Teaching Strategies

    ERIC Educational Resources Information Center

    Akerson, Valarie L.; Weiland, Ingrid; Rogers, Meredith Park; Pongsanon, Khemmawaddee; Bilican, Kader

    2014-01-01

    We explored adaptations to an elementary science methods course to determine how varied contexts could improve elementary preservice teachers' conceptions of NOS as well as their ideas for teaching NOS to elementary students. The contexts were (a) NOS Theme in which the course focused on the teaching of science through the consistent teaching…

  8. Safety evaluation report related to the operation of Watts Bar Nuclear Plant, Units 1 and 2 (Docket Nos. 50-390 and 50-391). Supplement No. 14

    SciTech Connect

    Tam, P.S.

    1994-12-01

    Supplement No. 14 to the Safety Evaluation Report for the application filed by the Tennessee Valley Authority for license to operate Watts Bar Nuclear Plant, Units 1 and 2, Docket Nos. 50-390 and 50-391, located in Rhea County, Tennessee, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation with additional information submitted by the applicant since Supplement No. 13 was issued, and matters that the staff had under review when Supplement No. 13 was issued.

  9. Safety evaluation report related to the operation of Watts Bar Nuclear Plant, Units 1 and 2 (Docket Nos. 50-390 and 50-391): Supplement No. 19

    SciTech Connect

    1995-11-01

    Supplement No. 19 to the Safety Evaluation Report for the application filed by the Tennessee Valley Authority for license to operate Watts Bar Nuclear Plant, Units 1 and 2, Docket Nos. 50-390 and 50-391, located in Rhea County Tennessee, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation with (1) additional information submitted by the applicant since Supplement No. 18 was issued, and (2) matters that the staff had under review when Supplement No. 18 was issued.

  10. Safety Evaluation Report related to the operation of Watts Bar Nuclear Plant, Units 1 and 2 (Docket Nos. 50-390 and 50-391). Supplement No. 12

    SciTech Connect

    Tam, P.S.

    1993-10-01

    Supplement No. 12 to the Safety Evaluation Report for the application filed by the Tennessee Valley Authority for license to operate Watts Bar Nuclear Plant, Units 1 and 2, Docket Nos. 50-390 and 50-391, located in Rhea County, Tennessee, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation of (1) additional information submitted by the applicant since Supplement No. 11 was issued, and (2) matters that the staff had under review when Supplement No. 11 was issued.

  11. Hazardous Material Data File (HMDF), quarterly update. Data file

    SciTech Connect

    Not Available

    1993-03-01

    The Hazardous Materiel Data File (HMDF) purpose is to provide storage and transportation information on chemical, radioactive and ammunition items in the Army inventory. Data is taken from the Army Central Logistics Data Bank (ACLDB).

  12. Hazardous Materiel Data File (HMDF), quarterly update. Data file

    SciTech Connect

    Not Available

    1990-05-01

    The Hazardous Materiel Data File (HMDF) purpose is to provide storage and transportation information on chemical, radioactive and ammunition items in the Army inventory. Data are taken from the Army Central Logistics Data Bank (ACLDB).

  13. 78 FR 10649 - Self-Regulatory Organizations; BATS Y-Exchange, Inc.; Notice of Filing and Immediate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-14

    .... 63097 (October 13, 2010), 75 FR 64767 (October 20, 2010) (SR-BYX-2010-002). \\6\\ Securities Exchange Act Release No. 64433 (May 6, 2011), 76 FR 27680 (May 12, 2011) (SR-BYX-2011-011). \\7\\ Securities Exchange Act Release No. 64735 (June 23, 2011), 76 FR 38243 (June 29, 2011) (File Nos. SR-BATS-2011-016; SR-BYX-...

  14. 77 FR 30566 - Self-Regulatory Organizations; NASDAQ OMX BX, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-23

    ... (January 13, 2004), 69 FR 2773 (January 20, 2004) (SR-BSE-2003-17); 49065 (January 13, 2004), 69 FR 2768 (January 20, 2004) (SR-BSE- 2003-04) (``BOXR Order''); and 49068 (January 13, 2004), 69 FR 2775 (January 20, 2004) (SR-BSE-2002-15). See also Release No. 34-58324; 73 FR 46936 (August 7, 2008) (File Nos....

  15. 76 FR 27675 - Self-Regulatory Organizations; New York Stock Exchange LLC; Notice of Filing of Proposed Rule...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-12

    ... Release No. 62252 (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos. SR-BATS-2010-014; SR-EDGA-2010... Securities Exchange Act Release No. 62251 (June 10, 2010), 75 FR 34183 (June 16, 2010) (SR-FINRA-2010-025..., 2010), 75 FR 34498 (June 17, 2010) (SR-NYSE-2010-45). \\4\\ The Commission approved the addition to...

  16. Tropospheric Emission Spectrometer Product File Readers

    NASA Technical Reports Server (NTRS)

    Fisher, Brendan M.

    2010-01-01

    TES Product File Reader software extracts data from publicly available Tropospheric Emission Spectrometer (TES) HDF (Hierarchical Data Format) product data files using publicly available format specifications for scientific analysis in IDL (interactive data language). In this innovation, the software returns data fields as simple arrays for a given file. A file name is provided, and the contents are returned as simple IDL variables.

  17. 77 FR 71412 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-30

    ... Company LLC submits Information Requested in Compliance with the October 31, 2012 Commission Order. Filed...: Order No. 587-V Revised Compliance Filing to be effective 12/1/2012. Filed Date: 11/21/12. Accession... 12/1/2012. Filed Date: 11/21/12. Accession Number: 20121121-5112. Comments Due: 5 p.m. ET...

  18. 40 CFR 78.4 - Filings.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 17 2014-07-01 2014-07-01 false Filings. 78.4 Section 78.4 Protection... § 78.4 Filings. (a)(1) All original filings made under this part shall be signed by the person making... requirements: (i) Any filings on behalf of owners and operators of a affected unit or affected source, TR...

  19. 40 CFR 78.4 - Filings.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 17 2012-07-01 2012-07-01 false Filings. 78.4 Section 78.4 Protection... § 78.4 Filings. (a)(1) All original filings made under this part shall be signed by the person making... requirements: (i) Any filings on behalf of owners and operators of a affected unit or affected source, TR...

  20. 47 CFR 1.1704 - Station files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Station files. 1.1704 Section 1.1704... System (COALS) § 1.1704 Station files. Applications, notifications, correspondence, electronic filings.... These files constitute the official records for these stations and supersede any other records,...

  1. 48 CFR 204.802 - Contract files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contract files. 204.802... OF DEFENSE GENERAL ADMINISTRATIVE MATTERS Contract Files 204.802 Contract files. Official contract files shall consist of— (1) Only original, authenticated or conformed copies of contractual...

  2. 10 CFR 603.1020 - File documents.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false File documents. 603.1020 Section 603.1020 Energy... Reporting Information About the Award § 603.1020 File documents. The award file should include an analysis..., the file must document the analysis required (see § 603.560) to set the fixed level of Federal...

  3. 48 CFR 1404.802 - Contract files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract files. 1404.802 Section 1404.802 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL ADMINISTRATIVE MATTERS Contract Files 1404.802 Contract files. In addition to the requirements in FAR 4.802, files...

  4. 18 CFR 1308.27 - Appeal files.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Appeal files. 1308.27... Board of Contract Appeals § 1308.27 Appeal files. (a) Notices of appeal shall be filed as provided in... an appeal file consisting of: (1) The Contracting Officer's decision, if any, from which the...

  5. 50 CFR 20.155 - Public file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Public file. 20.155 Section 20.155... § 20.155 Public file. (a) Establishment. A public file will be established for each rulemaking to which.... 552, a public file established pursuant to paragraph (a) of this section will contain: (1) The...

  6. 12 CFR 16.33 - Filing fees.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 1 2014-01-01 2014-01-01 false Filing fees. 16.33 Section 16.33 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY SECURITIES OFFERING DISCLOSURE RULES § 16.33 Filing fees. (a) Filing fees must accompany certain filings made under the provisions of this...

  7. 12 CFR 16.33 - Filing fees.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 1 2013-01-01 2013-01-01 false Filing fees. 16.33 Section 16.33 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY SECURITIES OFFERING DISCLOSURE RULES § 16.33 Filing fees. (a) Filing fees must accompany certain filings made under the provisions of this...

  8. 12 CFR 16.33 - Filing fees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Filing fees. 16.33 Section 16.33 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY SECURITIES OFFERING DISCLOSURE RULES § 16.33 Filing fees. (a) Filing fees must accompany certain filings made under the provisions of this...

  9. 12 CFR 16.33 - Filing fees.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 1 2011-01-01 2011-01-01 false Filing fees. 16.33 Section 16.33 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY SECURITIES OFFERING DISCLOSURE RULES § 16.33 Filing fees. (a) Filing fees must accompany certain filings made under the provisions of this...

  10. 48 CFR 4.802 - Contract files.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Contract files. 4.802 Section 4.802 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL ADMINISTRATIVE MATTERS Government Contract Files 4.802 Contract files. (a) A contract file should generally consist of— (1) The contracting office contract...

  11. 48 CFR 4.802 - Contract files.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Contract files. 4.802 Section 4.802 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL ADMINISTRATIVE MATTERS Government Contract Files 4.802 Contract files. (a) A contract file should generally consist of— (1) The contracting office contract...

  12. 77 FR 67355 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-09

    ... Energy Regulatory Commission Combined Notice of Filings Take notice that the Commission has received the.... The filings are accessible in the Commission's eLibrary system by clicking on the links or querying the docket number. eFiling is encouraged. More detailed information relating to filing...

  13. 78 FR 70033 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-22

    ... Energy Regulatory Commission Combined Notice of Filings Take notice that the Commission has received the... filings are accessible in the Commission's eLibrary system by clicking on the links or querying the docket number. eFiling is encouraged. More detailed information relating to filing requirements,...

  14. 78 FR 41049 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-09

    ... Energy Regulatory Commission Combined Notice of Filings Take notice that the Commission has received the.... The filings are accessible in the Commission's eLibrary system by clicking on the links or querying the docket number. eFiling is encouraged. More detailed information relating to filing...

  15. 78 FR 42515 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-16

    ... Energy Regulatory Commission Combined Notice of Filings Take notice that the Commission has received the.... The filings are accessible in the Commission's eLibrary system by clicking on the links or querying the docket number. eFiling is encouraged. More detailed information relating to filing...

  16. 20 CFR 217.16 - Filing date.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Filing date. 217.16 Section 217.16 Employees... LUMP SUM Filing An Application § 217.16 Filing date. An application filed in a manner and form... received will result in the loss or reduction of benefits. (e) On the date the Social...

  17. 12 CFR 303.121 - Filing procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Filing procedures. 303.121 Section 303.121 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES OF PRACTICE FILING PROCEDURES Activities of Insured State Banks § 303.121 Filing procedures. (a) Where to file. A notice...

  18. 5 CFR 1203.13 - Filing pleadings.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... delivery, by facsimile, or by e-filing in accordance with § 1201.14 of this chapter. If the document was... submitted by e-filing, it is considered to have been filed on the date of electronic submission. (e... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Filing pleadings. 1203.13 Section...

  19. 48 CFR 4.802 - Contract files.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Contract files. 4.802 Section 4.802 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL ADMINISTRATIVE MATTERS Government Contract Files 4.802 Contract files. (a) A contract file should generally consist of— (1) The contracting office contract...

  20. 5 CFR 2422.5 - Filing petitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Filing petitions. 2422.5 Section 2422.5... FEDERAL LABOR RELATIONS AUTHORITY REPRESENTATION PROCEEDINGS § 2422.5 Filing petitions. (a) Where to file. Petitions must be filed with the Regional Director for the region in which the unit or employee(s)...

  1. 50 CFR 20.155 - Public file.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 8 2011-10-01 2011-10-01 false Public file. 20.155 Section 20.155... § 20.155 Public file. (a) Establishment. A public file will be established for each rulemaking to which.... 552, a public file established pursuant to paragraph (a) of this section will contain: (1) The...

  2. 50 CFR 20.155 - Public file.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 9 2012-10-01 2012-10-01 false Public file. 20.155 Section 20.155... § 20.155 Public file. (a) Establishment. A public file will be established for each rulemaking to which.... 552, a public file established pursuant to paragraph (a) of this section will contain: (1) The...

  3. 48 CFR 4.802 - Contract files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Contract files. 4.802 Section 4.802 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL ADMINISTRATIVE MATTERS Government Contract Files 4.802 Contract files. (a) A contract file should generally consist of— (1) The contracting office contract...

  4. 77 FR 68760 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-16

    ... following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket...-001. ] Applicants: El Paso Natural Gas Company, L.L.C. Description: Non-Conforming Agreement Amendment.... Description: NWP NAESB 2.0 Compliance Filing--Second Substitute Sheet to be effective 12/1/2012. Filed...

  5. Probing the Hydrogen Bonding of the Ferrous–NO Heme Center of nNOS by Pulsed Electron Paramagnetic Resonance

    PubMed Central

    Astashkin, Andrei V.; Chen, Li; Elmore, Bradley O.; Kunwar, Deepak; Miao, Yubin; Li, Huiying; Poulos, Thomas L.; Roman, Linda J.; Feng, Changjian

    2015-01-01

    Oxidation of L-arginine (L-Arg) to nitric oxide (NO) by NO synthase (NOS) takes place at the heme active site. It is of current interest to study structures of the heme species that activates O2 and transforms the substrate. The NOS ferrous–NO complex is a close mimic of the obligatory ferric (hydro)peroxo intermediate in NOS catalysis. In this work, pulsed electron–nuclear double resonance (ENDOR) spectroscopy was used to probe the hydrogen bonding of the NO ligand in the ferrous–NO heme center of neuronal NOS (nNOS) without a substrate and with L-Arg or N-hydroxy-L-arginine (NOHA) substrates. Unexpectedly, no H-bonding interaction connecting the NO ligand to the active site water molecule or the Arg substrate was detected, in contrast to the results obtained by X-ray crystallography for the Arg-bound nNOS heme domain [Li et al. J. Biol. Inorg. Chem. 2006, 11, 753–768]. The nearby exchangeable proton in both the no-substrate and Arg-containing nNOS samples is located outside the H-bonding range and, on the basis of the obtained structural constraints, can belong to the active site water (or OH). On the contrary, in the NOHA-bound sample, the nearby exchangeable hydrogen forms an H-bond with the NO ligand (on the basis of its distance from the NO ligand and a nonzero isotropic hfi constant), but it does not belong to the active site water molecule because the water oxygen atom (detected by 17O ENDOR) is too far. This hydrogen should therefore come from the NOHA substrate, which is in agreement with the X-ray crystallography work [Li et al. Biochemistry 2009, 48, 10246–10254]. The nearby nonexchangeable hydrogen atom assigned as Hɛ of Phe584 was detected in all three samples. This hydrogen atom may have a stabilizing effect on the NO ligand and probably determines its position. PMID:26035438

  6. 4. Southwest fronts, dock nos. 491 and 492. Southeast end, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Southwest fronts, dock nos. 491 and 492. Southeast end, dock no. 492. View to north. - Offutt Air Force Base, Looking Glass Airborne Command Post, Nose Docks, On either side of Hangar Access Apron at Northwest end of Project Looking Glass Historic District, Bellevue, Sarpy County, NE

  7. Airfield setting of Facility Nos. 175 and 176, taken from ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Airfield setting of Facility Nos. 175 and 176, taken from north end of Ford Island Runway, with landplane hangars on the right - U.S. Naval Base, Pearl Harbor, Landplane Hangar Type, Wasp Boulevard and Gambier Bay Street, Pearl City, Honolulu County, HI

  8. Building Nos. 92, 381, and 392, view into common courtyard ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Building Nos. 92, 381, and 392, view into common courtyard between 92 (right), 391 (center deep), and 392 (left), view facing west-northwest - U.S. Naval Base, Pearl Harbor, Marine Railway No. 1 Accessories House & Apprentice Welding School, Additions, Intersection of Avenue B & Sixth Street, Pearl City, Honolulu County, HI

  9. 21. INTERIOR VIEW OF REFINING MILL, SHOWING LOCATIONS OF NOS. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. INTERIOR VIEW OF REFINING MILL, SHOWING LOCATIONS OF NOS. 1, 2, AND 3 MILLS, LOOKING SOUTH. SOME OF THE MACHINERY IN THIS SECTION HAS BEEN REMOVED. - Clay Spur Bentonite Plant & Camp, Refining Mill, Clay Spur Siding on Burlington Northern Railroad, Osage, Weston County, WY

  10. Las Rocas Nos Cuentan (Rocks Tell Their Stories)

    ERIC Educational Resources Information Center

    Llerandi-Roman, Pablo A.

    2012-01-01

    Many Earth science lessons today still focus on memorizing the names of rocks and minerals. This led the author to develop a lesson that reveals the fascinating stories told by rocks through the study of their physical properties. He first designed the lesson for Puerto Rican teachers, hence its Spanish title: "Las Rocas Nos Cuentan Su Historia."…

  11. AIRMEN'S BARRACKS (FACILITY Nos. 422, 442, AND 420) IN MIDDLE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AIRMEN'S BARRACKS (FACILITY Nos. 422, 442, AND 420) IN MIDDLE DISTANCE, ALSO SHOWING ESCOLTA AVENUE AT RIGHT, LOOKING SOUTHEAST FROM RESERVOIR HILL. (Part 2 of a 3 view panorama; see also CA-2398-4 and CA-2398-6.) - Hamilton Field, East of Nave Drive, Novato, Marin County, CA

  12. Tool nos. 277 and 2201, details for bending machine, Johnson ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Tool nos. 277 and 2201, details for bending machine, Johnson Company, Johnstown, Pa. Scale 3 inches - 1 ft, Feb 13th 1893, drawing number 15098. (Photograph of drawing held at the Johnstown Corporation General Office, Johnstown, Pennsylvania) - Johnson Steel Street Rail Company, 525 Central Avenue, Johnstown, Cambria County, PA

  13. Context view, Building Nos. 2729, with Building No. 28 in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Context view, Building Nos. 27-29, with Building No. 28 in the center, looking west at front of buildings, from a spot south of Building No. 29 - U.S. Veterans Hospital, Jefferson Barracks, Medical Officer in Charge Residence, VA Medical Center, Jefferson Barracks Division 1 Jefferson Barracks Drive, Saint Louis, Independent City, MO

  14. Context view, Building Nos. 2728, looking north from a spot ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Context view, Building Nos. 27-28, looking north from a spot south of Building No. 28 - U.S. Veterans Hospital, Jefferson Barracks, Medical Officer in Charge Residence, VA Medical Center, Jefferson Barracks Division 1 Jefferson Barracks Drive, Saint Louis, Independent City, MO

  15. Input data for inferring species distributions in Kyphosidae world-wide.

    PubMed

    Knudsen, Steen Wilhelm; Clements, Kendall D

    2016-09-01

    Input data files for inferring the relationship among the family Kyphosidae, as presented in (Knudsen and Clements, 2016) [1], is here provided together with resulting topologies, to allow the reader to explore the topologies in detail. The input data files comprise seven nexus-files with sequence alignments of mtDNA and nDNA markers for performing Bayesian analysis. A matrix of recoded character states inferred from the morphology examined in museum specimens representing Dichistiidae, Girellidae, Kyphosidae, Microcanthidae and Scorpididae, is also provided, and can be used for performing a parsimonious analysis to infer the relationship among these perciform families. The nucleotide input data files comprise both multiple and single representatives of the various species to allow for inference of the relationship among the species in Kyphosidae and between the families closely related to Kyphosidae. The '.xml'-files with various constrained relationships among the families potentially closely related to Kyphosidae are also provided to allow the reader to rerun and explore the results from the stepping-stone analysis. The resulting topologies are supplied in newick-file formats together with input data files for Bayesian analysis, together with '.xml'-files. Re-running the input data files in the appropriate software, will enable the reader to examine log-files and tree-files themselves. PMID:27508256

  16. VizieR Online Data Catalog: Namelists of variable stars Nos.67-73 (Khopolov+, 1985-97)

    NASA Astrophysics Data System (ADS)

    Kholopov, P. N.; Samus, N. N.; Kazarovets, E. V.; Perova, N. B.

    1997-09-01

    We present a computer-readable version of six Name-Lists of Variable Stars (Nos.67-72) published in the system introduced during the preparation of the 4th GCVS edition. For printed versions of these Name-Lists see IBVS No.2681,1985; No.3058,1987; No.3323,1989; No.3530,1990; No.3840,1993; No.4140,1995. The main body of the 4th GCVS edition is already available in computer-readable form (see , , ). There are 2735 stars in the Name-Lists Nos.67-72 (file "nl.dat"). The variables are arranged in the order of their names inside constellations. One star of the 67th Name-List (V1810 Cyg=No.67247) turned out to be non-existing, it has been excluded from the table. The identification table (file "ident.dat") and the list of references (file "ref.dat") follow. The total number of designated variable stars has now reached 31193. In the new computer-readable version 1.1 of the GCVS, vol. IV (catalog ), we now present identifications also for name-list stars. We make no special attempt to present the material in a completely homogeneous form. There happened minor style evolution from the 67th to the 70th Name-List: particularly, the 67th Name-List normally refers in the identification table only to the catalogues cited in the papers mentioned in the references in the files "nl.dat" and "ident.dat", and to the 70th Name-List it became a common practice to give more catalogue identifications. From the Name-List No.70, there is no completeness in identifications with the old catalogues of suspected variables (CSV), in many cases we consider the NSV identification sufficient. We also do not try to update the information of the older Name-Lists, we only put the information together and correct known mistakes. The corrections to the Name-Lists have been taken into account. It is necessary to reproduce here two remarks from forewords of the printed Name-Lists: Name-List No.69. In the case of the variable stars in the open cluster NGC 3766 (V843-V849 Cen

  17. 76 FR 39757 - Filing Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... contain all necessary metadata about a filing. (8) ``Electronic receipt'' means that an electronic... cross references, web links and image alternates. (e) Document security setting must have a PDF...

  18. File servers, networking, and supercomputers

    NASA Technical Reports Server (NTRS)

    Moore, Reagan W.

    1992-01-01

    One of the major tasks of a supercomputer center is managing the massive amount of data generated by application codes. A data flow analysis of the San Diego Supercomputer Center is presented that illustrates the hierarchical data buffering/caching capacity requirements and the associated I/O throughput requirements needed to sustain file service and archival storage. Usage paradigms are examined for both tightly-coupled and loosely-coupled file servers linked to the supercomputer by high-speed networks.

  19. File servers, networking, and supercomputers

    NASA Technical Reports Server (NTRS)

    Moore, Reagan W.

    1991-01-01

    One of the major tasks of a supercomputer center is managing the massive amount of data generated by application codes. A data flow analysis of the San Diego Supercomputer Center is presented that illustrates the hierarchical data buffering/caching capacity requirements and the associated I/O throughput requirements needed to sustain file service and archival storage. Usage paradigms are examined for both tightly-coupled and loosely-coupled file servers linked to the supercomputer by high-speed networks.

  20. Directory of astronomical data files

    NASA Technical Reports Server (NTRS)

    1978-01-01

    This Directory of Astronomical Data Files was prepared by the Data Task Force of the Interagency Coordination Committee for Astronomy (ICCA) in cooperation with the National Space Science Data Center (NSSDC). The purpose of the Directory is to provide a listing which will enable a user to locate stellar and extragalactic data sources keyed along with sufficient descriptive information to permit him to assess the value of the files for his use as well as the status and availability of the compilations.

  1. Sildenafil Ameliorates Gentamicin-Induced Nephrotoxicity in Rats: Role of iNOS and eNOS

    PubMed Central

    Morsy, Mohamed A.; Ibrahim, Salwa A.; Amin, Entesar F.; Kamel, Maha Y.; Rifaai, Rehab A.; Hassan, Magdy K.

    2014-01-01

    Gentamicin, an aminoglycoside antibiotic, is used for the treatment of serious Gram-negative infections. However, its usefulness is limited by its nephrotoxicity. Sildenafil, a selective phosphodiesterase-5 inhibitor, was reported to prevent or decrease tissue injury. The aim of this study is to evaluate the potential protective effects of sildenafil on gentamicin-induced nephrotoxicity in rats. Male Wistar rats were injected with gentamicin (100 mg/kg/day, i.p.) for 6 days with and without sildenafil. Sildenafil administration resulted in nephroprotective effect in gentamicin-intoxicated rats as it significantly decreased serum creatinine and urea, urinary albumin, and renal malondialdehyde and nitrite/nitrate levels, with a concomitant increase in renal catalase and superoxide dismutase activities compared to gentamicin-treated rats. Moreover, immunohistochemical examination revealed that sildenafil treatment markedly reduced inducible nitric oxide synthase (iNOS) expression, while expression of endothelial nitric oxide synthase (eNOS) was markedly enhanced. The protective effects of sildenafil were verified histopathologically. In conclusion, sildenafil protects rats against gentamicin-induced nephrotoxicity possibly, in part, through its antioxidant activity, inhibition of iNOS expression, and induction of eNOS production. PMID:25120567

  2. 77 FR 22566 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-16

    ... System Operator, Inc. submits tariff filing per 35: NYISO Compliance filing--Verification of MP Risk Mgmt.... submits tariff filing per 35.13(a)(2)(iii: Brazos NITSA NOA Filing to be effective 6/1/2012. Filed Date: 4... tariff filing per 35.13(a)(2)(iii: Filing of an Amended Interconnection Agreement to be effective...

  3. 75 FR 42435 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    .... Applicants: Entergy Arkansas, Inc. Description: Entergy Arkansas, Inc. submits tariff filing per 35.12: MBR... filing per 35.12: MBR and CBR Baseline Filing to be effective 7/14/ 2010. Filed Date: 07/14/2010... filing per 35.12: MBR and CBR Baseline Filing to be effective 7/14/2010. Filed Date:...

  4. 76 FR 16402 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-23

    ... of MBR Tariff to be effective 3/15/2011. Filed Date: 03/15/2011. Accession Number: 20110315-5001... tariff filing per 35: MBR Compliance Filing to be effective 3/2/2011. Filed Date: 03/15/2011. Accession... filing per 35: MBR Compliance Filing to be effective 3/2/2011. Filed Date: 03/15/2011. Accession...

  5. DNA evolution and successive file editions

    NASA Astrophysics Data System (ADS)

    Zebende, Gilney F.; Penna, Thadeu J. P.; Oliveira, Paulo Murilo C. de

    Sequences of nucleotides along DNA chains are known to present long range correlations. These correlations are small for simple species (algae) and increase for more complex ones. Scanning DNA chains one finds pieces called exons which are known to code some protein sequence, and others called introns whose usefulness is debatable and do not code protein sequences. By reading only exons (skipping introns), one always gets no correlation at all, in spite of observing a large correlation by reading the whole DNA sequence. The proposed explanation is that introns are fossil DNA parts no longer in use after evolutional replacement by new, better material (current exons). Sucessive editions of the files stored in a diskette follow the same dynamic mechanism proposed for DNA evolution. Current versions of the files play the role of exons, whereas introns correspond to old versions no longer in use (but still partially stored on the disk). We find that correlations indeed increase as more and more editions are performed. This artificial system has the advantage, over real DNA data, of allowing experiments.

  6. Effects of cyclooxygenase inhibitor pretreatment on nitric oxide production, nNOS and iNOS expression in rat cerebellum.

    PubMed

    Di Girolamo, G; Farina, M; Riberio, M L; Ogando, D; Aisemberg, J; de los Santos, A R; Martí, M L; Franchi, A M

    2003-07-01

    1. The therapeutic effect of nonsteroidal anti-inflammatory drugs (NSAIDs) is thought to be due mainly to its inhibition of cyclooxygenase (COX) enzymes, but there is a growing body of research that now demonstrates a variety of NSAIDs effects on cellular signal transduction pathways other than those involving prostaglandins. 2. Nitric oxide (NO) as a free radical and an agent that gives rise to highly toxic oxidants (peroxynitrile, nitric dioxide, nitron ion), becomes a cause of neuronal damage and death in some brain lesions such as Parkinson and Alzheimer disease, and Huntington's chorea. 3. In the present study, the in vivo effect of three NSAIDs (lysine clonixinate (LC), indomethacine (INDO) and meloxicam (MELO)) on NO production and nitric oxide synthase expression in rat cerebellar slices was analysed. Rats were treated with (a) saline, (b) lipopolysaccharide (LPS) (5 mg kg(-1), i.p.), (c) saline in combination with different doses of NSAIDs and (d) LPS in combination with different doses of NSAIDs and then killed 6 h after treatment. 4. NO synthesis, evaluated by Bred and Snyder technique, was increased by LPS. This augmentation was inhibited by coadministration of the three NSAIDs assayed. None of the NSAIDs tested was able to modify control NO synthesis. 5. Expression of iNOS and neural NOS (nNOS) was detected by Western blotting in control and LPS-treated rats. LC and INDO, but not MELO, were able to inhibit the expression of these enzymes. 6. Therefore, reduction of iNOS and nNOS levels in cerebellum may explain, in part, the anti-inflammatory effect of these NSAIDs and may also have importance in the prevention of NO-mediated neuronal injury. PMID:12871835

  7. Effects of cyclooxygenase inhibitor pretreatment on nitric oxide production, nNOS and iNOS expression in rat cerebellum

    PubMed Central

    DiGirolamo, G; Farina, M; Riberio, M L; Ogando, D; Aisemberg, J; de los Santos, A R; Martí, M L; Franchi, A M

    2003-01-01

    The therapeutic effect of nonsteroidal anti-inflammatory drugs (NSAIDs) is thought to be due mainly to its inhibition of cyclooxygenase (COX) enzymes, but there is a growing body of research that now demonstrates a variety of NSAIDs effects on cellular signal transduction pathways other than those involving prostaglandins. Nitric oxide (NO) as a free radical and an agent that gives rise to highly toxic oxidants (peroxynitrile, nitric dioxide, nitron ion), becomes a cause of neuronal damage and death in some brain lesions such as Parkinson and Alzheimer disease, and Huntington's chorea. In the present study, the in vivo effect of three NSAIDs (lysine clonixinate (LC), indomethacine (INDO) and meloxicam (MELO)) on NO production and nitric oxide synthase expression in rat cerebellar slices was analysed. Rats were treated with (a) saline, (b) lipopolysaccharide (LPS) (5 mg kg−1, i.p.), (c) saline in combination with different doses of NSAIDs and (d) LPS in combination with different doses of NSAIDs and then killed 6 h after treatment. NO synthesis, evaluated by Bred and Snyder technique, was increased by LPS. This augmentation was inhibited by coadministration of the three NSAIDs assayed. None of the NSAIDs tested was able to modify control NO synthesis. Expression of iNOS and neural NOS (nNOS) was detected by Western blotting in control and LPS-treated rats. LC and INDO, but not MELO, were able to inhibit the expression of these enzymes. Therefore, reduction of iNOS and nNOS levels in cerebellum may explain, in part, the anti-inflammatory effect of these NSAIDs and may also have importance in the prevention of NO-mediated neuronal injury. PMID:12871835

  8. Association of NOS2 and NOS3 gene polymorphisms with susceptibility to type 2 diabetes mellitus and diabetic nephropathy in the Chinese Han population.

    PubMed

    Chen, Feng; Li, Yu-Mei; Yang, Lin-Qing; Zhong, Cai-Gao; Zhuang, Zhi-Xiong

    2016-07-01

    Inducible nitric oxide synthase (NOS2) and endothelial nitric oxide synthase (NOS3) gene play important roles in the susceptibility to type 2 diabetes mellitus (T2DM). The present study aims to detect the potential association of NOS2 and NOS3 gene polymorphisms with the susceptibility toT2DM and diabetic nephropathy (DN) in the Chinese Han population. Four hundred and ninety T2DM patients and 485 healthy controls were enrolled in this case-control study. The genotypes of NOS2 and NOS3 gene polymorphisms were analyzed by the polymerase chain reaction (PCR)-ligase detection reaction (LDR) method. Our data demonstrated that the NOS2 rs2779248 and NOS2 rs1137933 genetic polymorphisms were significantly associated with the increased susceptibility to T2DM in the heterozygote comparison, dominant model, and allele contrast; and NOS3 rs3918188 genetic polymorphism was significantly associated with the increased susceptibility to T2DM in the homozygote comparison and recessive model. The allele-C and genotype-TC of NOS2 rs2779248, allele-A and genotype-GA of NOS2 rs1137933 and genotype-AA of NOS3 rs3918188 genetic polymorphisms might be the risk factors for increasing the susceptibility to T2DM. And a significant haplotype effect of NOS2 rs10459953/C- rs1137933/G- rs2779248/T was found between T2DM cases and controls. Moreover, NOS3 rs1800783 polymorphism was significantly associated with the increased susceptibility to DN in the heterozygote comparison, recessive model and allele contrast. At last, a positive correlation of family history of diabetes with NOS3 rs11771443 polymorphism was found in DN. These preliminary findings indicate that the NOS2 rs2779248, NOS2 rs1137933, and NOS3 rs3918188 genetic polymorphisms are potentially related to the susceptibility to T2DM, and the rs1800783 polymorphism might be considered as genetic risk factors for diabetic nephropathy, and family history of diabetes was closely associated with rs11771443 polymorphism in DN, and the

  9. The secret stridulatory file under the right tegmen in katydids (Orthoptera, Ensifera, Tettigonioidea).

    PubMed

    Chamorro-Rengifo, Juliana; Braun, Holger; Lopes-Andrade, Cristiano

    2014-01-01

    Males of most species of crickets and katydids produce species-specific calling songs to attract conspecific females. The typical stridulatory apparatus of the Ensifera consists of a file-and-scraper system in the basal dorsal region of the forewings (tegmina): the file on the underside of the cubital vein of one tegmen is composed of a series of lamelliform teeth and is run against the sclerotized scraper at the edge of the other tegmen. The region directly distal of the cubital vein is often thin and glassy and serves to amplify and spread the sound. In stridulating crickets the tegmina are quite symmetrical with both the left and the right one containing a file, which is considered the ancestral condition (Béthoux 2012). Most of these crickets adopted a right-over-left wing overlap and use only the right file. The few extant species of the ancient group Hagloidea have bilaterally symmetrical tegmina, both with functional files, and individual males can change the overlap (Morris & Gwynne 1978). Katydids are distinguished by a left-over-right wing overlap, with a stridulatory file on the underside of the left tegmen, and a scraper on the right one, which usually is also equipped with a mirror as resonating structure. PMID:24989770

  10. Zebra: A striped network file system

    NASA Technical Reports Server (NTRS)

    Hartman, John H.; Ousterhout, John K.

    1992-01-01

    The design of Zebra, a striped network file system, is presented. Zebra applies ideas from log-structured file system (LFS) and RAID research to network file systems, resulting in a network file system that has scalable performance, uses its servers efficiently even when its applications are using small files, and provides high availability. Zebra stripes file data across multiple servers, so that the file transfer rate is not limited by the performance of a single server. High availability is achieved by maintaining parity information for the file system. If a server fails its contents can be reconstructed using the contents of the remaining servers and the parity information. Zebra differs from existing striped file systems in the way it stripes file data: Zebra does not stripe on a per-file basis; instead it stripes the stream of bytes written by each client. Clients write to the servers in units called stripe fragments, which are analogous to segments in an LFS. Stripe fragments contain file blocks that were written recently, without regard to which file they belong. This method of striping has numerous advantages over per-file striping, including increased server efficiency, efficient parity computation, and elimination of parity update.

  11. Oxidative Stress Induced Ventricular Arrhythmia and Impairment of Cardiac Function in Nos1ap Deleted Mice.

    PubMed

    Sugiyama, Koji; Sasano, Tetsuo; Kurokawa, Junko; Takahashi, Kentaro; Okamura, Tadashi; Kato, Norihiro; Isobe, Mitsuaki; Furukawa, Tetsushi

    2016-05-25

    Genome-wide association study has identified that the genetic variations at NOS1AP (neuronal nitric oxide synthase-1 adaptor protein) were associated with QT interval and sudden cardiac death (SCD). However, the mechanism linking a genetic variant of NOS1AP and SCD is poorly understood. We used Nos1ap knockout mice (Nos1ap(-/-)) to determine the involvement of Nos1ap in SCD, paying special attention to oxidative stress.At baseline, a surface electrocardiogram (ECG) and ultrasound echocardiography (UCG) showed no difference between Nos1ap(-/-) and wild-type (WT) mice. Oxidative stress was induced by a single injection of doxorubicin (Dox, 25 mg/kg). After Dox injection, Nos1ap(-/-) showed significantly higher mortality than WT (93.3 versus 16.0% at day 14, P < 0.01). ECG showed significantly longer QTc in Nos1ap(-/-) than WT, and UCG revealed significant reduction of fractional shortening (%FS) only in Nos1ap(-/-) after Dox injection. Spontaneous ventricular tachyarrhythmias were documented by telemetry recording after Dox injection only in Nos1ap(-/-). Ex vivo optical mapping revealed that the action potential duration (APD)90 was prolonged at baseline in Nos1ap(-/-), and administration of Dox lengthened APD90 more in Nos1ap(-/-) than in WT. The expression of Bnp and the H2O2 level were higher in Nos1ap(-/-) after Dox injection. Nos1ap(-/-) showed a reduced amplitude of calcium transient in isolated cardiomyocytes after Dox injection. Administration of the antioxidant N-acetyl-L-cysteine significantly reduced mortality of Nos1ap(-/-) by Dox injection, accompanied by prevention of QT prolongation and a reduction in %FS.Although Nos1ap(-/-) mice have apparently normal hearts, oxidative stress evokes ventricular tachyarrhythmia and heart failure, which may cause sudden cardiac death. PMID:27170476

  12. 38. View of DRS 1, 2, and 3 (structure nos. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. View of DRS 1, 2, and 3 (structure nos. 735, 736, and 737) console fault locator for beam power status, radio frequency (RF) and intermediate frequency (IF) fault conditions, RF switches status and TR status. - Clear Air Force Station, Ballistic Missile Early Warning System Site II, One mile west of mile marker 293.5 on Parks Highway, 5 miles southwest of Anderson, Anderson, Denali Borough, AK

  13. Growth Hormone Effects in Immune Stress: AKT/eNOS Signaling Module in the Cellular Response

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The activation of the constitutive endothelial nitric-oxide synthase (eNOS) and expression of inducible NOS (iNOS) with subsequent nitric oxide production are among the early cellular responses that follow in a systemic exposure of animals to lipopolysaccharide (LPS). Growth hormone (GH) has been sh...

  14. 49 CFR 173.187 - Pyrophoric solids, metals or alloys, n.o.s.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Pyrophoric solids, metals or alloys, n.o.s. 173... Class 1 and Class 7 § 173.187 Pyrophoric solids, metals or alloys, n.o.s. Packagings for pyrophoric solids, metals, or alloys, n.o.s. must conform to the requirements of part 178 of this subchapter at...

  15. Expression, localization, and regulation of NOS in human mast cell lines: effects on leukotriene production.

    PubMed

    Gilchrist, Mark; McCauley, Scott D; Befus, A Dean

    2004-07-15

    Nitric oxide (NO) is a potent radical produced by nitric oxide synthase (NOS) and has pleiotrophic activities in health and disease. As mast cells (MCs) play a central role in both homeostasis and pathology, we investigated NOS expression and NO production in human MC populations. Endothelial NOS (eNOS) was ubiquitously expressed in both human MC lines and skin-derived MCs, while neuronal NOS (nNOS) was variably expressed in the MC populations studied. The inducible (iNOS) isoform was not detected in human MCs. Both growth factor-independent (HMC-1) and -dependent (LAD 2) MC lines showed predominant nuclear eNOS protein localization, with weaker cytoplasmic expression. nNOS showed exclusive cytoplasmic localization in HMC-1. Activation with Ca(2+) ionophore (A23187) or IgE-anti-IgE induced eNOS phosphorylation and translocation to the nucleus and nuclear and cytoplasmic NO formation. eNOS colocalizes with the leukotriene (LT)-initiating enzyme 5-lipoxygenase (5-LO) in the MC nucleus. The NO donor, S-nitrosoglutathione (SNOG), inhibited, whereas the NOS inhibitor, N(G)-nitro-l-arginine methyl ester (L-NAME), potentiated LT release in a dose-dependent manner. Thus, human MC lines produce NO in both cytoplasmic and nuclear compartments, and endogenously produced NO can regulate LT production by MCs. PMID:15044250

  16. 15 CFR Supplement Nos.1-3 to Part 746 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Nos.1 Supplement Nos.1-3 to Part 746 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF... CONTROLS Supplement Nos.1-3 to Part 746...

  17. High Rates of Psychiatric Co-Morbidity in PDD-NOS

    ERIC Educational Resources Information Center

    de Bruin, Esther I.; Ferdinand, Robert F.; Meester, Sjifra; de Nijs, Pieter F. A.; Verheij, Fop

    2007-01-01

    Rates of co-morbid psychiatric conditions in children with Pervasive Developmental Disorder-Not Otherwise Specified (PDD-NOS) are hardly available, although these conditions are often considered as more responsive to treatment than the core symptoms of PDD-NOS. Ninety-four children with PDD-NOS, aged 6-12 years were included. The DISC-IV-P was…

  18. Relationship between soil type and N₂O reductase genotype (nosZ) of indigenous soybean bradyrhizobia: nosZ-minus populations are dominant in Andosols.

    PubMed

    Shiina, Yoko; Itakura, Manabu; Choi, Hyunseok; Saeki, Yuichi; Hayatsu, Masahito; Minamisawa, Kiwamu

    2014-01-01

    Bradyrhizobium japonicum strains that have the nosZ gene, which encodes N2O reductase, are able to mitigate N2O emissions from soils (15). To examine the distribution of nosZ genotypes among Japanese indigenous soybean bradyrhizobia, we isolated bradyrhizobia from the root nodules of soybean plants inoculated with 32 different soils and analyzed their nosZ and nodC genotypes. The 1556 resultant isolates were classified into the nosZ+/nodC+ genotype (855 isolates) and nosZ-/nodC+ genotype (701 isolates). The 11 soil samples in which nosZ- isolates significantly dominated (P < 0.05; the χ(2) test) were all Andosols (a volcanic ash soil prevalent in agricultural fields in Japan), whereas the 17 soil samples in which nosZ+ isolates significantly dominated were mainly alluvial soils (non-volcanic ash soils). This result was supported by a principal component analysis of environmental factors: the dominance of the nosZ- genotype was positively correlated with total N, total C, and the phosphate absorption coefficient in the soils, which are soil properties typical of Andosols. Internal transcribed spacer sequencing of representative isolates showed that the nosZ+ and nosZ- isolates of B. japonicum fell mainly into the USDA110 (BJ1) and USDA6 (BJ2) groups, respectively. These results demonstrated that the group lacking nosZ was dominant in Andosols, which can be a target soil type for an N2O mitigation strategy in soybean fields. We herein discussed how the nosZ genotypes of soybean bradyrhizobia depended on soil types in terms of N2O respiration selection and genomic determinants for soil adaptation. PMID:25476067

  19. Exercise does not activate the β3 adrenergic receptor-eNOS pathway, but reduces inducible NOS expression to protect the heart of obese diabetic mice.

    PubMed

    Kleindienst, Adrien; Battault, Sylvain; Belaidi, Elise; Tanguy, Stephane; Rosselin, Marie; Boulghobra, Doria; Meyer, Gregory; Gayrard, Sandrine; Walther, Guillaume; Geny, Bernard; Durand, Gregory; Cazorla, Olivier; Reboul, Cyril

    2016-07-01

    Obesity and diabetes are associated with higher cardiac vulnerability to ischemia-reperfusion (IR). The cardioprotective effect of regular exercise has been attributed to β3-adrenergic receptor (β3AR) stimulation and increased endothelial nitric oxide synthase (eNOS) activation. Here, we evaluated the role of the β3AR-eNOS pathway and NOS isoforms in exercise-induced cardioprotection of C57Bl6 mice fed with high fat and sucrose diet (HFS) for 12 weeks and subjected or not to exercise training during the last 4 weeks (HFS-Ex). HFS animals were more sensitive to in vivo and ex vivo IR injuries than control (normal diet) and HFS-Ex mice. Cardioprotection in HFS-Ex mice was not associated with increased myocardial eNOS activation and NO metabolites storage, possibly due to the β3AR-eNOS pathway functional loss in their heart. Indeed, a selective β3AR agonist (BRL37344) increased eNOS activation and had a protective effect against IR in control, but not in HFS hearts. Moreover, iNOS expression, nitro-oxidative stress (protein s-nitrosylation and nitrotyrosination) and ROS production during early reperfusion were increased in HFS, but not in control mice. Exercise normalized iNOS level and reduced protein s-nitrosylation, nitrotyrosination and ROS production in HFS-Ex hearts during early reperfusion. The iNOS inhibitor 1400 W reduced in vivo infarct size in HFS mice to control levels, supporting the potential role of iNOS normalization in the cardioprotective effects of exercise training in HFS-Ex mice. Although the β3AR-eNOS pathway is defective in the heart of HFS mice, regular exercise can protect their heart against IR by reducing iNOS expression and nitro-oxidative stress. PMID:27164904

  20. Relationship Between Soil Type and N2O Reductase Genotype (nosZ) of Indigenous Soybean Bradyrhizobia: nosZ-minus Populations are Dominant in Andosols

    PubMed Central

    Shiina, Yoko; Itakura, Manabu; Choi, Hyunseok; Saeki, Yuichi; Hayatsu, Masahito; Minamisawa, Kiwamu

    2014-01-01

    Bradyrhizobium japonicum strains that have the nosZ gene, which encodes N2O reductase, are able to mitigate N2O emissions from soils (15). To examine the distribution of nosZ genotypes among Japanese indigenous soybean bradyrhizobia, we isolated bradyrhizobia from the root nodules of soybean plants inoculated with 32 different soils and analyzed their nosZ and nodC genotypes. The 1556 resultant isolates were classified into the nosZ+/nodC+ genotype (855 isolates) and nosZ−/nodC+ genotype (701 isolates). The 11 soil samples in which nosZ− isolates significantly dominated (P < 0.05; the χ2 test) were all Andosols (a volcanic ash soil prevalent in agricultural fields in Japan), whereas the 17 soil samples in which nosZ+ isolates significantly dominated were mainly alluvial soils (non-volcanic ash soils). This result was supported by a principal component analysis of environmental factors: the dominance of the nosZ− genotype was positively correlated with total N, total C, and the phosphate absorption coefficient in the soils, which are soil properties typical of Andosols. Internal transcribed spacer sequencing of representative isolates showed that the nosZ+ and nosZ− isolates of B. japonicum fell mainly into the USDA110 (BJ1) and USDA6 (BJ2) groups, respectively. These results demonstrated that the group lacking nosZ was dominant in Andosols, which can be a target soil type for an N2O mitigation strategy in soybean fields. We herein discussed how the nosZ genotypes of soybean bradyrhizobia depended on soil types in terms of N2O respiration selection and genomic determinants for soil adaptation. PMID:25476067

  1. Usage analysis of user files in UNIX

    NASA Technical Reports Server (NTRS)

    Devarakonda, Murthy V.; Iyer, Ravishankar K.

    1987-01-01

    Presented is a user-oriented analysis of short term file usage in a 4.2 BSD UNIX environment. The key aspect of this analysis is a characterization of users and files, which is a departure from the traditional approach of analyzing file references. Two characterization measures are employed: accesses-per-byte (combining fraction of a file referenced and number of references) and file size. This new approach is shown to distinguish differences in files as well as users, which cam be used in efficient file system design, and in creating realistic test workloads for simulations. A multi-stage gamma distribution is shown to closely model the file usage measures. Even though overall file sharing is small, some files belonging to a bulletin board system are accessed by many users, simultaneously and otherwise. Over 50% of users referenced files owned by other users, and over 80% of all files were involved in such references. Based on the differences in files and users, suggestions to improve the system performance were also made.

  2. The Different Potential of Sponge Bacterial Symbionts in N2 Release Indicated by the Phylogenetic Diversity and Abundance Analyses of Denitrification Genes, nirK and nosZ

    PubMed Central

    Zhang, Xia; He, Liming; Zhang, Fengli; Sun, Wei; Li, Zhiyong

    2013-01-01

    Nitrogen cycle is a critical biogeochemical process of the oceans. The nitrogen fixation by sponge cyanobacteria was early observed. Until recently, sponges were found to be able to release nitrogen gas. However the gene-level evidence for the role of bacterial symbionts from different species sponges in nitrogen gas release is limited. And meanwhile, the quanitative analysis of nitrogen cycle-related genes of sponge microbial symbionts is relatively lacking. The nirK gene encoding nitrite reductase which catalyzes soluble nitrite into gas NO and nosZ gene encoding nitrous oxide reductase which catalyzes N2O into N2 are two key functional genes in the complete denitrification pathway. In this study, using nirK and nosZ genes as markers, the potential of bacterial symbionts in six species of sponges in the release of N2 was investigated by phylogenetic analysis and real-time qPCR. As a result, totally, 2 OTUs of nirK and 5 OTUs of nosZ genes were detected by gene library-based saturated sequencing. Difference phylogenetic diversity of nirK and nosZ genes were observed at OTU level in sponges. Meanwhile, real-time qPCR analysis showed that Xestospongia testudinaria had the highest abundance of nosZ gene, while Cinachyrella sp. had the greatest abundance of nirK gene. Phylogenetic analysis showed that the nirK and nosZ genes were probably of Alpha-, Beta-, and Gammaproteobacteria origin. The results from this study suggest that the denitrification potential of bacteria varies among sponges because of the different phylogenetic diversity and relative abundance of nosZ and nirK genes in sponges. Totally, both the qualitative and quantitative analyses of nirK and nosZ genes indicated the different potential of sponge bacterial symbionts in the release of nitrogen gas. PMID:23762300

  3. 78 FR 78437 - Self-Regulatory Organizations; BOX Options Exchange LLC; Notice of Filing of Amendment Nos. 1 and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-26

    ... (September 17, 2013), 78 FR 58364 (``Notice''). \\4\\ See Securities Exchange Act Release No. 70799 (November 1, 2013), 78 FR 66980 (November 7, 2013). \\5\\ In Amendment No. 1, BOX added further information relating... Securities Exchange Act Release No. 70395 (September 15, 2013), 78 FR 57911 (September 20, 2013)) that...

  4. 75 FR 3263 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing of Amendment Nos. 2...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-20

    ... adequate procedures and controls that permit it to effectively monitor and control the Sponsored Access or... Direct Market Access or Sponsored Access to establish effective procedures and controls to systemically... establish a clear understanding of the regulatory and financial controls required in all access...

  5. 78 FR 4494 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing of Amendment Nos. 2 and 3...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ...\\ See Securities Exchange Act Release No. 68022 (October 9, 2012), 77 FR 62572 (``Notice''). \\5\\ See Securities Exchange Act Release No. 68313 (November 28, 2012), 77 FR 71853 (December 4, 2012). \\6\\ The..., Securities Exchange Act Release No. 64149 (March 30, 2011), 76 FR 18966 (April 6, 2011) (``Rule...

  6. 78 FR 4554 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Notice of Filing of Amendment Nos. 1...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ...\\ See Securities Exchange Act Release No. 68013 (October 9, 2012), 77 FR 62563 (``Notice''). \\4\\ See Securities Exchange Act Release No. 68313 (November 28, 2012), 77 FR 71853 (December 4, 2012). \\5\\ See..., Securities Exchange Act Release No. 64149 (March 30, 2011), 76 FR 18966 (April 6, 2011) (``Rule...

  7. 75 FR 41264 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing of Amendment Nos. 2 and 3, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ...., 75 FR at 20405. D. Integration of NYSE Amex-Listed Securities and Nasdaq Securities at Posts on the... almost exactly as the Exchange's listed securities.\\10\\ \\10\\ See Notice, supra note 3, 75 FR at 20405-06... Securities Exchange Act Release No. 61890 (April 12, 2010), 75 FR 20401 (``Notice''). II. Description of...

  8. Evaluated nuclear structure data file

    NASA Astrophysics Data System (ADS)

    Tuli, J. K.

    1996-02-01

    The Evaluated Nuclear Structure Data File (ENSDF) contains the evaluated nuclear properties of all known nuclides, as derived both from nuclear reaction and radioactive decay measurements. All experimental data are evaluated to create the adopted properties for each nuclide. ENSDF, together with other numeric and bibliographic files, can be accessed on-line through the INTERNET or modem, and some of the databases are also available on the World Wide Web. The structure and the scope of ENSDF are presented along with the on-line access system of the National Nuclear Data Center at Brookhaven National Laboratory.

  9. Evaluated nuclear structure data file

    NASA Astrophysics Data System (ADS)

    Tuli, J. K.

    The Evaluated Nuclear Structure Data File (ENSDF) contains the evaluated nuclear properties of all known nuclides. These properties are derived both from nuclear reaction and radioactive decay measurements. All experimental data are evaluated to create the adopted properties for each nuclide. ENSDF, together with other numeric and biographic files, can be accessed on-line through the INTERNET or modem. Some of the databases are also available on the World Wide Web. The structure and the scope of ENSDF are presented along with the on-line access system of the National Nuclear Data Center at Brookhaven National Laboratory.

  10. 78 FR 77446 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-23

    ... Agreements-- PNG & EGC to be effective 12/31/9998. Filed Date: 12/12/13. Accession Number: 20131212-5060...: Withdrawal Non-conforming & Negotiated Rate Service Agreements--PNG & EGC to be effective N/A. Filed Date:...

  11. Improving File System Performance by Striping

    NASA Technical Reports Server (NTRS)

    Lam, Terance L.; Kutler, Paul (Technical Monitor)

    1998-01-01

    This document discusses the performance and advantages of striped file systems on the SGI AD workstations. Performance of several striped file system configurations are compared and guidelines for optimal striping are recommended.

  12. 75 FR 31438 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... Lines Joint Service Agreement. Filing Party: Tara L. Leiter, Esq.; Blank Rome, LLP; Watergate; 600 New... Service Agreement. Filing Party: Tara L. Leiter, Esq.; Blank Rome, LLP; Watergate; 600 New...

  13. 78 FR 46935 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-02

    ...: 20130725-5032. Comments Due: 5 p.m. ET 8/6/13. Docket Numbers: RP13-1098-000. Applicants: Bison Pipeline LLC. Description: Bison Pipeline LLC submits tariff filing per 154.203: ACA Filing 2013 to...

  14. 78 FR 11633 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-19

    ... Numbers: RP13-553-000. Applicants: TC Offshore LLC. Description: Service Agmt--Housekeeping to be...: Northwest Pipeline GP. Description: Housekeeping Filing--CSOFO to be effective 4/1/2013. Filed Date:...

  15. 27 CFR 26.275 - Filing.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...' invoices, delivery receipts, bills or lading, etc., or exact copies of the same, may be filed in accordance... shall be filed not later than one business day following the date of transaction. (c) If an...

  16. 27 CFR 26.275 - Filing.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...' invoices, delivery receipts, bills or lading, etc., or exact copies of the same, may be filed in accordance... shall be filed not later than one business day following the date of transaction. (c) If an...

  17. 78 FR 64490 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-29

    .... Description: Eastman Chemical 410456 Negotiated Rate to be effective 11/1/2013. Filed Date: 10/16/13..., but intervention is necessary to become a party to the proceeding. eFiling is encouraged....

  18. Fail-over file transfer process

    NASA Technical Reports Server (NTRS)

    Semancik, Susan K. (Inventor); Conger, Annette M. (Inventor)

    2005-01-01

    The present invention provides a fail-over file transfer process to handle data file transfer when the transfer is unsuccessful in order to avoid unnecessary network congestion and enhance reliability in an automated data file transfer system. If a file cannot be delivered after attempting to send the file to a receiver up to a preset number of times, and the receiver has indicated the availability of other backup receiving locations, then the file delivery is automatically attempted to one of the backup receiving locations up to the preset number of times. Failure of the file transfer to one of the backup receiving locations results in a failure notification being sent to the receiver, and the receiver may retrieve the file from the location indicated in the failure notification when ready.

  19. 75 FR 57754 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-22

    ... 22, 2010. Docket Numbers: RP10-1289-000. Applicants: Bobcat Gas Storage. ] Description: Bobcat Gas Storage submits tariff filing per 154.203: Bobcat Baseline Tariff Filing to be effective 9/10/2010....

  20. 75 FR 79362 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-20

    ...-000. Applicants: Bobcat Gas Storage. Description: Bobcat Gas Storage submits tariff filing per 154.204...-000. Applicants: Bobcat Gas Storage. Description: Bobcat Gas Storage submits tariff filing per...